LOCUS       DATDAA010000001         4287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_139761,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000001 DATDAA010000000
VERSION     DATDAA010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139761"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VIS73_00005"
     CDS             complement(<1..929)
                     /locus_tag="VIS73_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5761568.1"
                     /translation="MTQLNTPYPSTAYLTGFLRSRAGELGVAAAQEDLALALVLRLFS
                     VDGLGWMHAGALALAAAGRTPGIDNFLAQFDRYRACIGPVVAFLQGRDPSLAQRIAGG
                     GFLPEGPRFAALDEYVDPDGGDPLAWAFGALGVQDRARHFATLFLDDLADVLRDAVDS
                     RFEFVRYAESLAMSQASFDPLAAALAAEPTAVDRLLDDLAVAALTRHQPALVLLSVPF
                     PGSVYGAFRIAQAIRRERPDVVTVLGGGYVNTELRELSEARVFDHFDYVTLDDGERPL
                     LALIDHLRGGRGRERLQRTFFREESGVVRYVGA"
     gene            1154..1678
                     /locus_tag="VIS73_00010"
     CDS             1154..1678
                     /locus_tag="VIS73_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761569.1"
                     /translation="MQAIHDIDALLLLATLLAAKRRPAELTELLAAAELINGAMPPTA
                     KLADAFARLGSNGLIQAAEGSDAGVAAVALTTQAQSLLTGRPPKRGEAAEQLATIKAQ
                     LGDYTPGGTHPPVQYSEAQLAAAIQSHQLAASAPGKNLLMPKPKPVETTAPRPGQRQR
                     KPLPSHRRKPGRDR"
     gene            1675..3099
                     /gene="dbpA"
                     /locus_tag="VIS73_00015"
     CDS             1675..3099
                     /gene="dbpA"
                     /locus_tag="VIS73_00015"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HEY5761570.1"
                     /translation="MSKPTAPAPAESTSFDSLPLAPAQQEILRQLEYRTMTPIQAASL
                     PLALAGRDLIAQAKTGSGKTAAFALPLLARLNQRHFAVQALVLCPTRELAEQVSQEIR
                     RLARFASNIKVLTLCGGSTMRPQMSSLEHGAHVVVGTPGRIMDHLERGSLRLDDLNTL
                     VLDEADRMLDMGFHDDIVYVAKRCPAQRQTLLFSATYPERIAELAARFLRQPETIKLT
                     EQHASTKIRQRFYEVSHNDRLQAVATLLRHYRPVSTLAFCNTRQQCRDLLEVLRAQGI
                     DALALHGELEQRERDQVLIQFANRSCSVLVATDVAARGLDIDRLEAVINVDMTPDPEI
                     HIHRIGRTGRADEDGWALSLCSPADRRRVARFGEQLGGEPEWHTLGSLQGGDAMPLVP
                     PMATLLILGGRKEKIRPADVLGALTGDGGNTRPLTREQVGKITVTDLNTYVAVSRELA
                     GDAVKKLTKGKLKGKTVKVRSLSD"
     gene            3273..4124
                     /gene="rhlP"
                     /locus_tag="VIS73_00020"
     CDS             3273..4124
                     /gene="rhlP"
                     /locus_tag="VIS73_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04179.1"
                     /note="RhlP (RHombo-target LipoProtein) is a family of
                     predicted lipoproteins that, in general, co-occurs with a
                     form of rhombosortase, and that has an apparent cleavage
                     site for that enzyme, a GlyGly motif, near the C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhombotarget lipoprotein"
                     /protein_id="HEY5761571.1"
                     /translation="MIPRTPRTGTLLAVTLSIALTACAGLMGQQKNQQNASVMDYLYP
                     KKSQAAAMAPAATVDLKLPLRVGIAFVPGGYGGDTALPENERQKLLERVKAAFLDQQF
                     ISAIEVVPTAYLRPQGGFENLEQASRMFNVDVVALLSYDQVQFNDANRLSLLYWTIIG
                     AYMVNGDQYDTHTLIDASVFDVRSRKLLMRAPGTSQVKGSAALVNFSEKSRDARIAGY
                     GQAVDALIPNLRSELEKFRERVKTDQTIRVTHSPGYSGGGDLGLAGLLLALAAVAVLA
                     VRRRGDA"
BASE COUNT          804 a         1516 c         1352 g          615 t
ORIGIN      
        1 ctggcgccga cgtagcgcac gacgcccgac tcctcgcgga agaaggttcg ctgcaggcgc
       61 tcgcggccgc gtccgccgcg cagatgatcg atcagcgcta gcagcggccg ctcgccgtcg
      121 tcgagcgtga cgtagtcgaa gtgatcgaag acgcgcgctt ccgacagctc gcgcagttcg
      181 gtattgacgt agccaccgcc gagcacggtg acgacatcgg ggcgttcgcg ccggatcgcc
      241 tgcgcaatgc ggaaggcccc gtagaccgag cccggaaacg gcaccgagag cagcaccagc
      301 gccggctggt ggcgtgtcaa cgcggcaacc gccaaatcat cgagcaggcg gtcgaccgcc
      361 gtcggctcgg cagcaagcgc cgccgccagt ggatcgaaac tcgcttggct catcgccaac
      421 gactcggcgt agcggacgaa ctcgaaacgc gaatcgacgg catcgcgcag cacgtcggcg
      481 agatcgtcga ggaacagcgt agcgaaatgg cgggcccggt cctggacgcc gagcgcaccg
      541 aaagcccagg ccagcggatc gccgccatcg gggtcgacgt actcgtcgag cgcggcgaag
      601 cgcggtccct cgggcaggaa gccaccaccg gcgatgcgct gcgccagcga cggatcgcgg
      661 ccctggagga aggcgacgac cggcccgatg caggcgcgat agcggtcgaa ttgcgcgagg
      721 aaattatcga ttccaggggt ccgcccggcg gccgccaagg ccagcgcgcc cgcgtgcatc
      781 cagcccagcc cgtcgaccga aaacagccgc aggaccagcg ccagcgccag atcctcctgc
      841 gccgccgcga cgcccaattc gccggcccgc gaacgcagaa atccggtcag gtacgccgtt
      901 gacgggtacg gcgtattgag ttgcgtcatc ggcggaatca gcgacaggat tcgcagcggc
      961 cggggagcgg aaaaggacac gggcggggct ttgggcgacg gcaagagctt gggcctgcga
     1021 tgatacggca gagcgccggc cccgacggca cccggcgaac tggtacgatc cgcccccgcc
     1081 cccaaaacca cccaccgccc gcacgccatt ggccgattcg cccgaagcgc cgcgccggcc
     1141 ccacaggaat ccgatgcaag ccatccacga tatagacgcc ctgctcctgc tcgccacgct
     1201 gctggctgcg aagcggcgcc ccgccgaact gaccgagctg ctggcagccg ccgagctgat
     1261 caacggcgcg atgccgccta ccgcaaaact cgccgacgcc ttcgcccgcc ttggcagcaa
     1321 cggcctgatc caggccgccg aaggcagcga tgccggggtc gccgccgttg cactgacgac
     1381 gcaggctcag tcgctgctga ccgggcggcc gccgaaacgc ggcgaagcgg ccgagcagct
     1441 ggcgacgatc aaggcccagc tcggcgacta cacgcccggc ggcacgcatc cgcccgtgca
     1501 gtacagcgaa gcgcaactcg ccgcagcgat tcagtcgcat cagctcgccg ccagcgcccc
     1561 cggcaagaac ctgttgatgc caaagccgaa gcccgtcgaa accacggccc cgcgccccgg
     1621 ccagcgccag cgcaagccgc tgcctagcca tcgccgcaaa cccgggcgag accggtgagc
     1681 aaacccacgg cgccggcgcc tgcggaatcg acgtccttcg actccctgcc gctcgcgccg
     1741 gcgcaacaag agatcctgcg ccagcttgaa taccggacga tgacaccgat ccaggccgcc
     1801 agcctgccgc tggcgctcgc cggaagagac ctgatcgcac aggcgaagac cggcagcggc
     1861 aagacggcgg cgttcgcgct gccgctgctg gcccgcctca atcaacggca cttcgccgta
     1921 caggcattgg tgctctgccc gacgcgcgaa ctcgccgaac aggtcagcca ggaaatccgc
     1981 cgtctggcgc gcttcgccag caacatcaag gtgctcacgc tgtgcggcgg ctcgacaatg
     2041 cgaccgcaga tgtccagcct cgaacacggc gcgcacgtcg tcgtcggcac gccgggacgc
     2101 atcatggatc acctcgaacg cggcagcctg cggctcgacg acctgaacac gctggtgctc
     2161 gacgaggccg atcgcatgct cgacatgggg ttccacgacg acatcgtcta cgtcgccaag
     2221 cgttgcccgg cgcaacggca gacactgctg ttctcggcga cctaccccga gcgcatcgcc
     2281 gaactcgccg cgcgcttcct gcgccagccg gaaaccatca agctcaccga gcagcacgcc
     2341 agcaccaaga tccgccagcg cttctacgag gtctcgcaca acgatcgcct gcaggcggtc
     2401 gccacgctgc tcaggcatta ccggccggta agcacgctgg ccttctgcaa cacccgccag
     2461 caatgccgcg acctgctcga agtactgcgc gcgcagggta tcgacgcgct ggcgctgcac
     2521 ggcgaactcg aacagcgaga gcgcgatcag gtgctgatcc agttcgccaa tcgcagctgc
     2581 tcggtgctcg tcgccaccga cgtcgccgcc cgcggactcg acatcgaccg gctcgaagcg
     2641 gtcatcaatg tcgacatgac ccccgatccc gaaatccata tccaccgcat cggccgcacc
     2701 ggccgcgccg acgaggatgg ctgggcgcta tcgctgtgca gtccggccga tcgccgccgc
     2761 gtcgcgcgct tcggcgagca gttgggcggc gagcccgaat ggcacacgct gggttcgctg
     2821 cagggtggtg acgccatgcc tctggtgccg ccgatggcga cgctgctgat cctcggcgga
     2881 cgcaaggaaa agatccgccc tgccgacgtg ctcggcgcgc taaccggcga cggcggcaac
     2941 acccgcccgc tgacgcgcga acaggtcgga aagattaccg tcaccgacct caatacctac
     3001 gtcgccgttt cccgcgaact agctggcgac gccgtcaaga aactcaccaa gggaaagctc
     3061 aagggcaaga ccgtcaaggt gcggtcactc agcgactgag ccaaccgggg cggcattgac
     3121 tgcgacgctc acgacgcgtc gctgccggat ctggcgatta ggcaggtcaa ctttttcgta
     3181 gatcgagctt gaccttgtcc ttcagtatgc tcagattact ttgacgagct tcggtgtttg
     3241 ctgctgggcg accatctacg aaaggacact cgatgattcc ccgtacacca aggaccggga
     3301 cgctgctggc cgtgacattg tcgatcgcgc ttaccgcttg tgcaggcctg atggggcagc
     3361 aaaagaacca gcagaacgcc agcgtgatgg actacctcta tcccaagaaa agtcaggcgg
     3421 cggcgatggc tccagcggcg accgtcgatc tcaagctgcc ccttcgcgtc ggcatcgcat
     3481 tcgttcccgg cggatatggc ggcgacaccg cactgcccga gaacgaacgc cagaaactcc
     3541 tcgaacgggt caaggccgcc ttccttgatc agcagttcat ttcggcgatc gaggtcgtac
     3601 cgacggctta tttgcggccg cagggtggct tcgagaacct cgaacaggcg tcgcggatgt
     3661 tcaacgtcga cgtcgttgcg ctgctttcgt acgaccaggt ccagttcaat gacgccaatc
     3721 gcctgtcgct gctttactgg accatcatcg gtgcctacat ggtcaatggc gaccagtacg
     3781 acacacacac gctgatcgac gcgtcggtat tcgacgtcag aagccgcaaa ttgctgatgc
     3841 gcgcgccggg aaccagccag gtcaagggca gcgcggcact cgtcaatttc agcgagaagt
     3901 cacgcgacgc ccgcatcgct ggctacggcc aggccgtcga cgcgctgatc ccgaatctgc
     3961 gcagcgagtt ggagaagttc cgggagcgcg tcaagaccga ccagacgatc cgcgtcaccc
     4021 actcgcctgg ctattcgggc ggcggcgatc ttggcctcgc tggcctgcta ctggcgcttg
     4081 ctgcggtcgc cgtgctcgcg gtgcgccgcc gcggcgatgc ctgacattac gcaccccgcc
     4141 tgatgccgcg ctggcttgtg gtgctaccgg gcctcgcctg cctgatgctg gccgccttgc
     4201 ccgagggcgc ccaagtctgg ctcgcttggc agcgcgacgc catcacgcaa ggcgagatct
     4261 ggcgcctgtg gaccgcgcat ctgcttc
//
LOCUS       DATDAA010000002         3893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_314460,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000002 DATDAA010000000
VERSION     DATDAA010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314460"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VIS73_00025"
     CDS             <1..433
                     /locus_tag="VIS73_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEY5761572.1"
                     /translation="NTGNVIRLCANAGCELHLIEPLGFAWDDRQLKRAGLDYHEFTPV
                     ARHRDLAACQAALGERRWFAMTTKGACRIGQDAPAFADDDVFLFGPESRGLPADVLAQ
                     FPAERRLRLPMRAGQRSLNLSNAVAVTVFEAWRQHGYAGAV"
     gene            complement(447..1433)
                     /locus_tag="VIS73_00030"
     CDS             complement(447..1433)
                     /locus_tag="VIS73_00030"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY5761573.1"
                     /translation="MTIAVLGAGAWGTALAIAWAADRPVRLWSREQDHVAAMLAAGEN
                     AAFLPGYRLPDALIPTGDFTAAVSGADIAVIATPVAGLRETAAALCERRFAGRLVWVC
                     KGFETGSGLLPHAVVAEAFPGAAGGALSGPSFAQEVADGRPTAVTLAAPDLADAEALA
                     RELHTGSFRVYAHDDLPGTEIGGAIKNVLAIATGICDGLGLGYNARAALITRGLAEIA
                     RLGAALGGRRETFMGLAGVGDLILTCTGDLSRNRRVGLELAQGKTLDEILVSLGHVAE
                     GVNTAREVARLATARQIDMPIARAVAAVLDGKLAAKAAVGELLARAPTVEFR"
     gene            complement(1486..1965)
                     /gene="secB"
                     /locus_tag="VIS73_00035"
     CDS             complement(1486..1965)
                     /gene="secB"
                     /locus_tag="VIS73_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEY5761574.1"
                     /translation="MNDAPVFAIEKLYVKDFSVEVPNAPQIFLDRESPEIEVQLRSQA
                     RGVDEGMFEVLLTVTVTAKVKDKVAFLVEVGQAGIFQVKNVPDESVEPLLGIACPNIL
                     FPYAREAVSEATVRAGFQSVVLQPVNFESLFMSQKEAQQGGDKQDGDKQDAPQIVLP"
     gene            complement(2026..2283)
                     /gene="grxC"
                     /locus_tag="VIS73_00040"
     CDS             complement(2026..2283)
                     /gene="grxC"
                     /locus_tag="VIS73_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEY5761575.1"
                     /translation="MAKILMYSTAVCPYCIRAEQLLKSKGVAEIEKVRIDLDPVRQQE
                     MIERTGRRTVPQIYIGETHVGGCDDLYALDRAGKLDPLLAA"
     gene            complement(2324..2749)
                     /locus_tag="VIS73_00045"
     CDS             complement(2324..2749)
                     /locus_tag="VIS73_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY5761576.1"
                     /translation="MPIDFLKQNILLIALVATSGGMLLWPLIFRSGAKAVSAAEATML
                     INREDAVVLDVRSAAEFADGHIPGAINVPVERVADKLAELESYRARPVIVACQSGVRT
                     GGACGKLKKGGFERLYELTGGVGSWKQAGLPIKKGKSGR"
     gene            complement(2847..3167)
                     /locus_tag="VIS73_00050"
     CDS             complement(2847..3167)
                     /locus_tag="VIS73_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5761577.1"
                     /translation="MDKPTFNLIEKQEHIDQAARALKAIAHPLRLKILCVVGDQEACV
                     QEIVDAVGTSQSNISQHLAILREKDVLLTRKEANRVYYRVGDERTLQLIGMMREVFCG
                     VDAK"
     gene            3342..>3893
                     /locus_tag="VIS73_00055"
     CDS             3342..>3893
                     /locus_tag="VIS73_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761578.1"
                     /translation="MIRVTLVLLAWAALLAPPAASAASDPNDGKTPSAAELSARRDDL
                     QSLRSRISRLQRDIAGTESTRREAADELRQSEREISRLRREQRRLSRERQVVESELRR
                     LEAESRSASRRLDGQTGQIGALLRRQLRYGDLDPLRLALSGLPPAEAARNRLLLARIA
                     ATRAKIASDTRSDLQQTETLAAQT"
BASE COUNT          620 a         1305 c         1332 g          636 t
ORIGIN      
        1 gaataccggc aacgtcattc gcctgtgtgc caatgccggt tgcgagttgc acctgatcga
       61 gccgctgggc tttgcttggg atgaccggca gctcaagcgc gccggccttg actaccacga
      121 gttcacgccg gttgcacggc atcgcgatct cgctgcctgt caggcggcgc tgggcgagcg
      181 gcgctggttc gcgatgacga ccaagggcgc ctgccgcatc ggccaagacg ccccggcctt
      241 cgccgacgac gacgtgttcc tgttcggccc cgagtcacgc ggcctgccag ccgacgtact
      301 ggcgcagttt ccggccgaac gccgcctgcg actgccgatg cgagccgggc aacgcagtct
      361 caatctttcc aatgcggtgg cggtgacggt cttcgaggcc tggcggcagc acggctacgc
      421 cggcgccgtc tgagccgggg aagccctcag cgaaactcga cggtgggcgc gcgggcgagc
      481 agttcgccga ccgccgcctt cgccgcgagc ttgccgtcga gcaccgcggc gacggcgcgg
      541 gcaatcggca tgtcgatctg ccgtgccgtg gccagccgcg ccacttcacg cgccgtgttg
      601 acgccttcgg cgacgtggcc gagcgaaact agtatctcgt cgagcgtctt gccctgcgcc
      661 agttcgaggc caacgcggcg gttgcgcgag aggtcgccgg tgcaggtgag gatgaggtcg
      721 ccgacgccgg ccaatcccat gaaggtttcg cggcgaccgc cgagcgcggc gccgagtcgg
      781 gcgatctcgg cgagtccgcg cgtgatcagg gcggcgcggg cgttgtagcc cagaccgagg
      841 ccatcgcaaa tgccggtggc gatggccagc acgttcttga tcgcgccgcc gatctcggtg
      901 cccggcaagt cgtcgtgcgc gtagacgcgg aagctgccgg tgtgcagctc gcgcgccagc
      961 gcctcggcgt cggccaggtc gggggcggcc agcgtgaccg ccgtgggccg gccgtcggcg
     1021 acttcctggg caaagctcgg tccgctcagc gcgccgcccg cggcaccggg aaaggcctcg
     1081 gcgacgacgg cgtgcggcaa caatccgctg ccggtctcga accccttgca gacccagacc
     1141 aggcgcccgg caaagcggcg ctcgcacaag gccgccgccg tctcgcgcag accggcgacc
     1201 ggcgtggcga tgacggcaat gtcggcgccc gacaccgctg cggtgaaatc gccggtggga
     1261 atcaaggcat caggcagccg atagccgggc aggaaggccg cgttctcgcc ggccgccagc
     1321 atcgccgcga cgtgatcctg ctcgcgcgac cagagtcgga ccggccggtc ggcggcccag
     1381 gcgatcgcca atgcggtacc ccaggcgccg gcgccgagga cggcgatggt cacgcggagt
     1441 ccccggtgcg attcgaacgg cggcgctgac cggctggcat tgcgatcagg gaaggacgat
     1501 ctgcggtgcg tcctgcttgt cgccgtcctg cttgtcaccg ccctgctgcg cttccttctg
     1561 cgacatgaac agcgactcga agttgaccgg ctgcagaacg accgactgga atccggcacg
     1621 caccgtcgcc tcggacaccg cttcgcgggc gtaggggaag aggatgttcg ggcaggcgat
     1681 gccgagcagc ggctcgacgc tctcgtcggg gacgttcttg acctggaaga tgccggcctg
     1741 cccgacctcg acgaggaagg cgaccttgtc cttgaccttg gccgtcacgg tgacggtcag
     1801 cagcacctcg aacatgccct cgtcgacgcc gcgcgcctgc gaacgcagct ggacctcgat
     1861 ctccgggctt tcgcggtcga gaaagatttg cggtgcgttg ggcacctcga ccgagaaatc
     1921 cttgacgtag agcttctcga tggcaaatac gggggcgtcg ttcatggcag tttccaatga
     1981 cagtggtgaa acgggaggct gccgcgcgtc agcgacagca gcggatcagg cagcgagcag
     2041 cggatcgagc ttgccggcgc ggtcgagcgc gtagagatcg tcgcagccgc caacgtgggt
     2101 ctcgccgatg tagatctgcg gcaccgtacg gcggccggtc cgttcgatca tttcctgctg
     2161 gcggacggga tcgaggtcga tgcggacctt ctcgatctcg gcgacgccct tgctcttgag
     2221 cagttgttcg gcgcggatgc agtaggggca gaccgcggtg ctgtacatga ggattttcgc
     2281 catcgacgct tccttggtat ttcgttgtgt tcggcgagac ggctcagcgc ccgctcttgc
     2341 ccttcttgat cggaaggccg gcctgtttcc aggagccgac gccgcccgtg agttcgtaga
     2401 ggcgctcgaa gccgcccttc ttcagcttgc cgcaggcgcc gccggtacgc acacctgact
     2461 ggcaggcgac gatcacgggc cgggcgcgat agctttcgag ttcggccagc ttgtcggcga
     2521 cgcgctcgac gggaacattg atcgctcccg gaatgtgtcc atcggcaaat tcggcggccg
     2581 agcggacgtc gagcacgacc gcgtcctcgc ggttgatcag catcgtcgcc tcggcagccg
     2641 agaccgcctt ggcgccggag cggaagatca gtggccagag cagcatgccg ccactggttg
     2701 ccaccaaggc gatgagcagg atgttttgtt tgagaaaatc gatgggcatg aaaataagtc
     2761 ctggggaatt cggcgcctgg cgccacgacg ggtgaaattc gggaatacgg ggctgacgat
     2821 tcgcaggcag aaaggcgctg ctgctgctat ttggcgtcga cgccgcagaa aacctcgcgc
     2881 atcatgccga tcagctgcag ggtccgctcg tcgcccaccc ggtaatagac gcgattggct
     2941 tccttgcgcg tgagcagaac gtccttctcg cggaggatgg ccagatgctg cgagatattg
     3001 ctctgcgaag tccccacggc atcgacgatc tcctgcacgc aggcctcctg gtcgccgacg
     3061 acgcagagga tcttcagccg cagcggatgg gctatcgcct tgagcgcccg cgcggcctga
     3121 tcgatgtgtt cctgcttctc gatcaggttg aaagtcggct tgtccaaggg gcgcgctccg
     3181 cagcaggaat tggcggcaac ggccgcgaac agggcggatt ataaaatacg cggcaccatc
     3241 ctagtgagct ttcccccgat gcttccgccc tcccccgccc gattgcccat ttcgagtacc
     3301 ggcttttcgc cctcgttgca gcctgtcggc ggcaagccgc agtgattcgt gtgactctgg
     3361 tgctgctggc gtgggcggcc ctgctggcgc cgcctgctgc ctcggcagcg agcgacccga
     3421 acgacggcaa gacgccctcg gcggccgaac tgagcgcccg ccgcgacgat ttgcagtcgc
     3481 tgcgctcgcg catcagccgc ctgcaacgcg acattgccgg caccgaatcg acacggcgcg
     3541 aggccgccga tgagttgcgg caatccgagc gcgagatctc gcgcctgcgc cgcgagcagc
     3601 gccgcctgtc ccgagagcgg caggtcgtgg agagcgagtt gcgccgcctc gaggccgaga
     3661 gccgcagcgc cagccggcgc ctcgacgggc agaccggaca gatcggcgca ctgctgcgcc
     3721 gccagctgcg ctacggtgac cttgatccgc tgcggctcgc actgtcgggc ctgccgcccg
     3781 ccgaagcggc gcgcaaccgc ttgttgctcg cccgcatcgc cgccacgcgc gccaagatcg
     3841 ccagcgacac gcgctccgac ctgcagcaga ccgaaaccct cgccgcacag acg
//
LOCUS       DATDAA010000003        20651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_419311,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000003 DATDAA010000000
VERSION     DATDAA010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 20651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20651
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419311"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VIS73_00060"
     CDS             complement(<1..347)
                     /locus_tag="VIS73_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HEY5761579.1"
                     /translation="MAIPREGNLEAPTRHVIDWKSPDFHNEESLHQEMERVFEICHGC
                     RRCVSLCQSFPTLFDLIDESESMEIDGVAKADYEKVVDHCYLCDLCYMTKCPYTPPHE
                     WNLDFPHLMLRAKA"
     gene            complement(418..834)
                     /locus_tag="VIS73_00065"
     CDS             complement(418..834)
                     /locus_tag="VIS73_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEY5761580.1"
                     /translation="MQLKGSKTEQNLKDAFAGESQANRRYLYFASKADVEGYNDVAAL
                     FRSTAEGETGHAHGHLEYLEKTGDPATGMPFGPTADNLRAAVAGETHEYTDMYPGMAK
                     TARDEGFEEIADWFETLAKAERSHANRYQKALNELG"
     gene            1291..2364
                     /locus_tag="VIS73_00070"
     CDS             1291..2364
                     /locus_tag="VIS73_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HEY5761581.1"
                     /translation="MTATQTTLLPAPRVEALKTPYATRRVPREAFATLLTDAKPAVGD
                     LVLARVKALGQHKHLELASGRRAKLFPGDEIVLAYGNRYAPDQFEAEIPEDLGPCHMV
                     AGGGIASRMIDKHARMSGATEIEPVGLIGDRSGRVINLADYALPKLQPAKFRPFTVAV
                     VGTSMNAGKTDTVTHTIRGLREAGYRVGAAKVTGTGSGGDAWSMVDAGAFEVLDFSDA
                     GLPSTYLASIEQCENVTTTLVAELTKNGAQTIVFEVADGLFQRETAQLLESPVFRSQV
                     DAVIFAAGDAMGAAAGVSWLRARNLPVVAASGLMTASPLAAREAADAAGIAILTREDL
                     ASATLADRLGVGESFRAAISAAG"
     gene            2367..4055
                     /locus_tag="VIS73_00075"
     CDS             2367..4055
                     /locus_tag="VIS73_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5761582.1"
                     /translation="MASTAALPPILAPGRRGLLARLVANGILQAATMWGTAKLTHLAV
                     ESMALSGDANAMRWLALAAGLLASALVLLGLRAVERTDGERMGQRYTAELRMVLFDHL
                     ARVSSRELQRRRQGSLLLRFVGDLKAMRLWVSLGFSRVIVASLSIAGVTLGLFIAAPR
                     LAIASCIALLLCAAIAQLLAVRTRIAMRDARYRQSMLAANVSEKVTSMAVLQLFGQVA
                     RERARIVRQAKRLESAATAQAQYSALLRALGDTAAMLASGAVLLTGGWLAAAGSLDAG
                     AAIAAMVLAGLQAPALRDLGLALGYRASADVSREKFRQFLARHPQLIETPNSTAIVPT
                     EGRLELRGVSLGNVFDDLSAVFEPGRIHLIRGANGSGKSTLLALAARLMQPERGEVLL
                     DGQTLAAHRLDSVHRSIGMAGPDLPLLRGTLDANLRYRWRRAPAAELQRIRQLCGIDE
                     LAATLPDGLQTRIDEGGRNLSPGQRQRVMLARALLGNPAVLLLDEVDSNLDPQAESIL
                     DDVLRNYAGTVLMVSHRQPRLPASQVVWTIANKKLVKETETNHDHRPERLHRVA"
     gene            4018..5130
                     /locus_tag="VIS73_00080"
     CDS             4018..5130
                     /locus_tag="VIS73_00080"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEY5761583.1"
                     /translation="MITAPNASIALPEATADALLRPLDALFDSLEGFFARDTSRVARR
                     APRVGVVEEMRLASDPAQRYFLFIPRGDSARRRIFVAIHGISRNADEHAAEFSRLAER
                     HGLVIVAPLFDETRFPDYQRLGRSGKGERADLMLDRIVAEVAQLSGTDGTRFHLFGYS
                     GGGQFAHRYALAHPERVVAYAIGAAGWYTLPDAAQKYPLGIRSTERLPDLAFDPRRFL
                     AIPAAVFVGDRDVHDGTALRQSERLRAEQGENRHERGRTWIDAMTHAARSLAMETEFH
                     FESLPRSPHCFLKSMRRGGLGERVVAWMFDRPQEVRPPLCGAVTLSLVPAFTAERIAP
                     KAILSCKANGDPATGATMRASPAGASRIRTCAAGPP"
     gene            complement(5102..6157)
                     /locus_tag="VIS73_00085"
     CDS             complement(5102..6157)
                     /locus_tag="VIS73_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY5761584.1"
                     /translation="MRKVMGMAGIALAFLAVSAPVPASAFEKSIEFYGVVDVSLGVEK
                     AGAVKRIGFEGGELDGSRIGVKGKAAFSDDFTGVFVLEAGFDPGTGQSEQDDRLFGRQ
                     AYAGVEGAFGRVTGGRQYSPAFVALDPLEATGGAARSVGILHRTSDSVRRGYNVRFDD
                     MVKYRTPSWGGFFIDAGYWVGDENASAESQVRKEGSGSGVAAVFDGEILSVAAVTQRR
                     FVNDTGGLATTRGGGVALDFGPVKLFAQATRDRESGSAGTGEARTASVGVRFRILGKN
                     TLALSVARRDESGEPAATDVSGASLYYLRALDRETTMYLAYSRLANKVGAKYRWNFDP
                     QPGEAVAAVMVGLRHKF"
     gene            complement(6253..8247)
                     /locus_tag="VIS73_00090"
     CDS             complement(6253..8247)
                     /locus_tag="VIS73_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY5761585.1"
                     /translation="MQGLEILPRAKAVACLLLGLLAPAAAYAERIEASYFVDLPIVLT
                     PSRLPQPQNEAPAAVTVIDREMIRATGYEDIPRLLRLVPGMQVGQERGGKHWVTYHGM
                     GNDYPSWMQVLVDGRSVFSPANFDGVDWSSLPVTVDDIERIEVVRGTNSTAYGSNAVL
                     GVVNIITRHSGDLDGSAASVSAGNQGRRGGAAQWRGAGERGSVRLNASFRGDDGFDDL
                     HDSHRTSIVSLRGDYRPGNRDELTALLGASRGQQQLGYADSLFNNNGEREADFSSTHV
                     LLQWRHTPRSIEEWSFSYYHNNDEVTEAWQAAAPPLVTGVPLDRNRESQRDSVEFQHR
                     LSWSDAVRTVWGGELRRDAITAPFLYAGQPDQHQHLGRLFGQIENRLTPSVTTTASGL
                     VERFDDHEKKFSPRFFVNWQASRHDAWRAGYARAWRGPFIFERRGDVQVTQPGFGLLA
                     QPYAPNIALQPSRMDSIEIGYLGEIPDWDARVDVRLFRERIDGYIARAALPSAGLLGL
                     PTARYENLATPVELRGVEYQIDHRPWAGGQFLFSHALIDRRSDDTLVRRLTAAHTASL
                     SYFQNHRRWSGALSILRMGSIAGGSGFVPTTEYASPGYHTVDFRLAYALEPTVEIALA
                     GINIGPRHQEIADRSEQALHGTTPVNLTSPMVWLTISVRE"
     gene            complement(8354..9064)
                     /locus_tag="VIS73_00095"
     CDS             complement(8354..9064)
                     /locus_tag="VIS73_00095"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEY5761586.1"
                     /translation="MISLDRLLQREGFGSRRDCRRLVNAGLVAVEGAACDNPDAPFAS
                     DGVAVRVDGVDWVCREHAVLMLNKPTGFECSRSPTHHPSVLTLLPPQLARRGLQPVGR
                     LDADTAGLLIVTDDGALNHRLTSPRHGAAKRYAVTLKHDADADLGERLMAGVVLRDSP
                     AAVAASEVAIESPRRLLLTIQEGRYHQVKRMIAAAGNRVEALERLAVGGLSLPGDLPR
                     GQWRWLTEGEMAQLTAPA"
     gene            complement(9061..10008)
                     /locus_tag="VIS73_00100"
     CDS             complement(9061..10008)
                     /locus_tag="VIS73_00100"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5761587.1"
                     /translation="MPEKRPAQPPQPAARTSFLPSPRQLRYLVAVADCLSFTHAAEAC
                     CVSQPSLSAGIKELENGLGVKLFERDRSRVLLTAIGESLVARARRLLAETEDLVALAT
                     TAARPMTGVLHFGAIPTIAPFVLPGLVASLRESAPELRLMLREDTSARLADQLRDGSL
                     DFAVLALPFPLDDLHVEQLYDDPLVVVSAVGAPMPRPPIAPESLDAGSLILLEEGHCL
                     REHTLVACSAGQAATQRRGIEASSLITLVQMVAGGLGIALMPQMAIGSGLIESAGLVV
                     RKLKPPPVRGIALVARPTSTKSAEFAAIGKTLKTLAKAS"
     gene            10206..10706
                     /gene="napF"
                     /locus_tag="VIS73_00105"
     CDS             10206..10706
                     /gene="napF"
                     /locus_tag="VIS73_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HEY5761588.1"
                     /translation="MLTRRQLLRGDFLAKAYSLAPPWAVRHFTSACSRCDACVTACPT
                     GLLIRGDGGFPRTDYSRSECTFCGDCATACKDDALRRGDGQAPWSRRILIDDSCLTRI
                     GVVCRSCGEHCDAQAIRFVAQAGGVANPSLDAAACTGCGACVAVCPNRAIRVVANTAE
                     FSRRAA"
     gene            10703..10975
                     /locus_tag="VIS73_00110"
     CDS             10703..10975
                     /locus_tag="VIS73_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HEY5761589.1"
                     /translation="MNISSVVLRAQPARIPEIRDEIARRPGHEIHGEHEAGRLALTIE
                     DSDTRSAADAYVALHDIPGVIAVSLIYQYSDDETATAEPAAETIAN"
     gene            10992..13526
                     /gene="napA"
                     /locus_tag="VIS73_00115"
     CDS             10992..13526
                     /gene="napA"
                     /locus_tag="VIS73_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HEY5761590.1"
                     /translation="MKLTRRDFIKTQAAAAAVAAAGLPATSVVAADKADDIRWDKAPC
                     RFCGTGCSVLVGTKDGRVVATQGDPDAPVNRGLNCIKGYFLSKIQYGADRLTSPMLRK
                     KNGKYDKNGEFTPVSWDEAFDIMAEKWKATLKAKGPTGVAMFGSGQWTVWEGYAAAKL
                     FKAGFRSNNIDPNARHCMASAVAGFMRTFGIDEPMGCYDDLENADAFVLWGSNMAEMH
                     PILWSRLTDRKLSAKHVKVHVLSTFEHRSFELADNAMIFKPQTDLAILNYICNHIIKS
                     GKVNKDFVAKHVNFKKGETDIGFGLRPNHALEKVATNNGYPGEDSKPKGNPGKADPIT
                     FEEFAKFVSEYTVEKASKISGVPVENLKRLAELYADPKAKITSYWTMGFNQHTRGTWV
                     NNMIYNVHLLTGKISEPGNGPFSLTGQPSACGTAREVGTFAHRLPADMVVMNPEHRAK
                     SEKIWQLPEGTIPDKPGTHAVLMARALKDKKVNVYWQMCNNNMQAGPNINDELFPGWR
                     DPENFIVVSDPYPTVSAMAADLVLPTAMWVEKEGAYGNAERRTQFWRQQVKGPGQSQS
                     DLWQVVEFSKRFKMEEVWPAELLAKAPQYKGKTLYDVLFRNGVVNKFPVANCTQVNGN
                     VIANYVNDESKHFGYYLQKGLFEEYAAFGRGKAHDLADFDTYHHARGLRWPVVDGKET
                     LWRFREGYDPYVAKGEDVKFYGHKDGKAVIFALPYQDPPEMPDKDFDLWLCTGRVLEH
                     WHTGTMTRRVPELHRSFPEAVLFMHPDDAKERGLQRGMVAKIASRRGEIELYVETRGR
                     NKPPKGLVFVPFFDAGRLINKLTLDATCPISKETDFKKCAVKVVKA"
     gene            13553..14350
                     /gene="napG"
                     /locus_tag="VIS73_00120"
     CDS             13553..14350
                     /gene="napG"
                     /locus_tag="VIS73_00120"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HEY5761591.1"
                     /translation="MVSETRRPASPDRRQFIAGSLGALGGATLFGLGVGLVAREAGAL
                     PADAIRPPGALSEDRFSSACVRCGLCVRDCPYDTLKLGVIGDGVGPGTPFFNARAIPC
                     EMCEDIPCVKACPTGALDPALKDIDNSRMGLAVLADQETCLNFLGLRCDVCYRVCPLI
                     DTAITLETRRNPRSDRHALLIPTVHSDACTGCGKCEHACVLEEAAIKIFPIALAKGKL
                     GSHYRKGWEEKAKAGGSLLEDMPAPEARRPDAPRVDPQLETLGGGLR"
     gene            14347..15219
                     /gene="napH"
                     /locus_tag="VIS73_00125"
     CDS             14347..15219
                     /gene="napH"
                     /locus_tag="VIS73_00125"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HEY5761592.1"
                     /translation="MSFIVAATDMPGRTASAARGWLAANRYLLMRRASQLAVLALFLL
                     GPWAGIWLIKGNLSASLLLDTVPMTDPLLMAQALAARHWPEATAWIGAGVVVGFYLLV
                     GGRSFCAWVCPLNIVTDTSAWLRRRLGLKGGTAPSRRLRHWLLLALLIAAFASGRMVW
                     EWVNPVSMTQRALIFGGAGGLAVALGIFLYDLLVAPRGWCGHVCPTGAAYALIGRASL
                     TRVAAARRERCDDCLDCFAVCPEPQVIRPALKGTGSPLILDSDCTNCGRCIDVCAPSV
                     FRLTHRFDTRRDAS"
     gene            15216..15662
                     /locus_tag="VIS73_00130"
     CDS             15216..15662
                     /locus_tag="VIS73_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HEY5761593.1"
                     /translation="MKTLINRKATILFAACLAFAMPISAQEKLVSERGDVPINAESKP
                     EPYKIERDRDPIPRDYVQQPPLIPHSTKGYSITQDFNKCLDCHSWTRYKEFNATKVSL
                     THFKDRDGKELANVSPRRYFCLQCHVVQFDAKPLVENQFRPVAGVR"
     gene            15687..16304
                     /locus_tag="VIS73_00135"
     CDS             15687..16304
                     /locus_tag="VIS73_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEY5761594.1"
                     /translation="MIKEILNKVKALGWPLAAGLFTAGIIFWGGFNWALEATNTEQFC
                     ISCHEMRENVFKEYKNTVHYTNRTGVRATCPDCHVPKEWVYKIKRKIQASNEVWHKLL
                     GSIDTPEKFHAKRAQLAENEWRRMRATDSRECRNCHDFEFMDYTEQGRRAGKAHPEGF
                     AEGKTCIDCHKGIAHALPPIDQDIGDRNIGATKISHGETAKDKGY"
     gene            complement(16690..17355)
                     /locus_tag="VIS73_00140"
     CDS             complement(16690..17355)
                     /locus_tag="VIS73_00140"
                     /inference="COORDINATES: protein motif:HMM:NF022620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HEY5761595.1"
                     /translation="MTTRRGVIGRTALVLGLLALAGCQTAYYGAMEKLGYEKRDLLVG
                     NVEDAREAQEAAKKQFASALEQFVAVTGFDGGDLEKQYNTLKDEYEDSEARANDVRKE
                     IAEVERVATDLFAEWEKELSEYQSAELRLASQKQLQDTKGRYRQLIGAMRAAEKKLDP
                     VLAAFKDRVLFLKHNLNARAIASLRGQRATVEADIGALITDMNKAIAEADRFIASMRS
                     PGS"
     gene            complement(17486..18187)
                     /locus_tag="VIS73_00145"
     CDS             complement(17486..18187)
                     /locus_tag="VIS73_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY5761596.1"
                     /translation="MRTIGLIGGMSWESTLPYYRIINETVRDRLGGLHSAKIVLVSID
                     FHEVEALQRAGEWEAAGRLLVEAGQTLVTAGADAVVLCTNTMHKLADPLAAALRVPLL
                     HIADPTAATIAEHGLRTVGLLGTRFTMEEDFYRARLQQRHGLGVVVPDAPDRELVHRV
                     IYDELCQGRLLDNSRAAYRAVIARLVGRGAQGIILGCTEIALLVGADDSPVPMFDTAA
                     IHARSAAEWAIGDER"
     gene            complement(18335..18511)
                     /locus_tag="VIS73_00150"
     CDS             complement(18335..18511)
                     /locus_tag="VIS73_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761597.1"
                     /translation="MSMADSCKREFKKKNADHTGRIPIPVVTCSTSTATQGESFAANW
                     KSVTADCAADRAAG"
     gene            18504..19397
                     /locus_tag="VIS73_00155"
     CDS             18504..19397
                     /locus_tag="VIS73_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEY5761598.1"
                     /translation="MLKPLSLSVLALIVGLGFAPSDAEAKRLGGARSFGMQRQATPPA
                     KAPAAAPAPTQAPAGAPAAQPKRSWMGPIAGLAAGLGLAALASHLGLGEELASMLLIG
                     LLIVGGIIVFRLLTRRASPAPRPAMAGASPYAAHEPPAAPTERAPLAGSHGQAMPGSV
                     ADSFAKPDSAAPRIPADFDVVAFSRNAKVNFVRLQAANDAGDLDDLRAFTTPEMFAEL
                     KMDIAERGSAPQHTDVVEIDAEVIEAVEEGSRYVVSVRFRGQLREEEDGPLVTIDELW
                     HLVKPREGKAGWLLAGIQQTQ"
     gene            19499..20647
                     /locus_tag="VIS73_00160"
     CDS             19499..20647
                     /locus_tag="VIS73_00160"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY5761599.1"
                     /translation="MPFGRLSWWPCLFVLTAMALAACGGRDDYGSSSNPPPAGSAPGT
                     ITLATTGLATGSSGALAITGPGGFVANIPTTQTSVAVPGPGDYTITAAEVLNGSRVLA
                     PQAATQTITVRSGTTAAATVAYRDDRALALSLQQVVTSLVSPLYLTSPTGDARLFVVE
                     QAGTIRIISGGVLLPTPFLSIPNPSRLLAGGERGLLSMAFDPQYAANGFFYVYLTDQN
                     GDIAIERYQVSASNANSADPTPLRLLTISHRDFANHNGGLLAFGPDGMLYAATGDGGG
                     GGDPLGSGQNLGSLLGKLLRIDVRNASSGTLYVVPPDNPFVAQAGARPETWAYGLRNP
                     WRFAFDPPAGRLYIADVGQNRIEEINAVAAATAGLNYGWNLTSSPPSA"
BASE COUNT         3592 a         7131 c         6438 g         3490 t
ORIGIN      
        1 gccttggccc gcagcatcag gtgcggaaaa tcgagattcc actcgtgcgg cggcgtgtac
       61 ggacacttgg tcatgtagca gagatcgcaa aggtagcagt ggtcgacgac cttctcgtag
      121 tcggccttgg cgacgccgtc aatctccatg ctttccgact cgtcgatgag atcgaacaag
      181 gtcgggaacg actggcacag gctgacgcaa cggcggcagc cgtgacagat ctcgaagaca
      241 cgttccatct cctggtgcag cgattcctcg ttgtgaaaat cggggctttt ccagtcgatt
      301 acgtgacgcg tcggtgcctc cagattgcct tcgcgcggaa tggccatggt gctctccctg
      361 tttcgccagc gatgccattg cgcgcccggc ctcccgggcc ggacgcgtac ggcgtgatca
      421 gccgagttcg ttcagtgcct tctggtagcg gttggcgtgc gagcgttcgg ccttggccag
      481 cgtctcgaac cagtcggcga tttcctcgaa gccttcgtcg cgcgcggtct tggccatccc
      541 cgggtacatg tcggtgtact cgtgggtctc gccggcgaca gcggccctaa ggttgtcggc
      601 ggtcggaccg aagggcattc cagtggccgg atcgccggtt ttctcgaggt actcgaggtg
      661 tccgtgcgcg tgcccggtct cgccctcggc ggtggagcgg aacagcgccg cgacgtcgtt
      721 gtagccttca acgtcggcct tcgatgcgaa gtacaggtag cgacggttgg cctgcgattc
      781 gccggcgaag gcgtctttaa ggttctgttc ggttttcgat cccttgagct gcatggctgt
      841 ctcctgaatg ggtttcacga tgcaggacag ctgcgtcctg actcactcat cataggcagg
      901 acgaccttcg ctgtgaaatc gattgtggct gtgctgttta taggcttgcg ctatcgcaaa
      961 caacggtgct ttcgcccttg aaagcgcgcc cggggacaca ggttttaggc attgacagag
     1021 ttaccgctgc cgcaagccgg gccaccgctc tcaactgggt gaagtggggc aaatagcgca
     1081 acctctcggg tagccaggcc cgtttccccc aattccaata gagcggagcg cttccagacg
     1141 acattccaga aatcatggca accatatgtt tttccacggt aataggtaat ttccactcga
     1201 tctcaacgtc attggcacgc cgcgtgctac ttgttttccg tgggattttt ttccacaccc
     1261 gaaaaatctg accgcccacg gagaataagc atgaccgcca cccagaccac cctgctcccc
     1321 gccccgcgcg tagaagcgct caagactccc tacgcaaccc gccgcgtgcc acgcgaagcc
     1381 tttgcgacgc tgctaaccga cgccaagccg gcggtcggcg atctcgttct cgcacgggtc
     1441 aaggcactcg gccagcacaa gcatctggaa ctggcctccg gccgccgcgc caagctattt
     1501 ccgggcgacg agatcgtgct tgcctatggc aaccgctacg cgccagacca gttcgaggcg
     1561 gaaatccccg aggacctcgg tccctgtcac atggtcgccg gcggcggtat cgcctcgcgc
     1621 atgatcgaca agcacgcgcg catgtcgggc gcaacggaaa tcgagccggt cggcctgatc
     1681 ggcgatcgca gcggccgcgt gatcaatctc gccgattacg cactgccgaa gttgcagccc
     1741 gcaaaattcc gtcccttcac cgtcgccgtg gtcggcacct cgatgaacgc cgggaagacc
     1801 gatacggtga cccacaccat ccgcgggttg cgcgaggcgg gttaccgtgt cggcgcggcc
     1861 aaggtaaccg gcaccggttc cggtggcgat gcctggtcaa tggtcgatgc cggcgccttc
     1921 gaggtcctcg acttcagcga tgccggtctc ccctcgacct acctcgcatc aatcgagcaa
     1981 tgcgaaaacg tgaccacgac gctggtcgcc gagctaacca agaacggcgc gcaaactatc
     2041 gtttttgaag tcgcagacgg tctctttcag cgcgagaccg cccaactgct cgaatcgccg
     2101 gtgttccgct cgcaagtcga tgccgtgatc ttcgccgccg gcgacgcgat gggggcagcc
     2161 gccggcgtca gctggctgcg cgcgcgcaac ctgccggtcg tcgccgccag cggcttgatg
     2221 accgcctcgc cacttgccgc ccgcgaggct gccgacgcgg ctggcatcgc gattctgacg
     2281 cgcgaagatc tggcatcggc aacgctcgcc gaccggctcg gcgtcggcga atccttccgc
     2341 gccgctatct cggccgcagg ctgatcgtgg cttccaccgc cgccctcccg ccgattctcg
     2401 cgccaggccg ccgggggttg ctcgcccggc tggtggccaa cggcattctg caggcggcga
     2461 cgatgtgggg caccgcgaag ctgacccatc tcgctgttga atcgatggca ctgtcaggcg
     2521 acgccaatgc aatgcgctgg ctggccctgg ccgccggcct gttggcgagc gccctcgtgt
     2581 tgctcggctt acgtgcggtc gagcgcacgg acggcgagcg gatgggccag cgctacaccg
     2641 ccgaactgcg gatggtcctc ttcgaccatc tggcgcgcgt ctcctcgcgt gaactgcaac
     2701 ggcgccggca gggcagtttg ctgctgcgct tcgtcggcga cctcaaggcc atgcgcctgt
     2761 gggtcagcct tggtttctcc cgcgtcatcg tcgccagcct tagcatcgcc ggcgtgacac
     2821 tcggcctttt catcgccgcg ccacggctgg ctatcgcctc gtgcatcgcg ttgctgcttt
     2881 gcgctgcgat cgcccagtta ctcgcagtgc ggactcgaat cgccatgcgc gacgcccgat
     2941 accggcagtc aatgctcgcc gcgaacgtca gcgagaaggt cacctcaatg gccgttctgc
     3001 aactcttcgg acaagtcgcc cgcgagcgcg cccggatcgt ccggcaggcg aagcgcctcg
     3061 aatccgctgc caccgcccaa gcgcagtaca gcgccctgct ccgcgcgctc ggcgataccg
     3121 ccgcgatgct cgcatccggc gcggtactgc tgaccggcgg ctggctcgcc gccgcaggtt
     3181 cgctcgacgc cggcgcggct atcgccgcaa tggtgctcgc cggcctccag gcgccggcct
     3241 tgcgcgatct gggcctggca ctgggttatc gcgctagcgc cgacgtctcg cgcgagaagt
     3301 tccgccagtt cctggcccgc catccgcagc tgattgaaac gcccaattcc accgcaatcg
     3361 tccccacgga ggggcgcctc gaattgcgcg gcgtatcgct cggcaatgtc ttcgacgatc
     3421 tttcggccgt gtttgagccc ggacgaattc atctcattcg cggcgccaac ggctcgggca
     3481 agtccacctt gctcgcgctt gccgcgcggc tgatgcaacc ggagcgcggc gaggtcctgc
     3541 tcgacggcca gacgctcgct gcacaccgcc tcgactcagt gcatcgctcc atcggcatgg
     3601 ccggccccga tctcccgctg ctgcgcggaa cgctcgacgc caacctgcgc taccgctggc
     3661 ggcgggcacc cgcggcagag ctgcaacgca tccggcaact gtgcgggatc gacgaactgg
     3721 cggcaacgct ccccgacggc ctgcagacac gcatcgacga gggtgggcgc aatctgtcgc
     3781 caggccagcg gcagcgcgtc atgttggcgc gtgcgctgct cggcaatccg gcggtcctgc
     3841 tcctggacga ggtcgactca aatctcgatc cacaggccga aagcattctc gatgacgtcc
     3901 tccgcaacta tgcaggcacc gtgctgatgg tctcgcatcg ccaaccgcgc ctgcccgcat
     3961 cccaagtcgt ctggacaatc gccaacaaga agctcgtcaa agaaacagag accaatcatg
     4021 atcaccgccc cgaacgcctc catcgcgttg cctgaagcga ccgccgacgc tctgctgcgg
     4081 ccgctcgacg cgctcttcga cagccttgag ggctttttcg cgcgcgatac cagccgcgtc
     4141 gcgcggcgcg cgccacgggt tggcgtcgtc gaggaaatgc gcctggccag cgacccggcg
     4201 cagcgttatt tcctgttcat tccccgcggc gattccgcgc gacggcggat tttcgtcgcc
     4261 atccacggca tcagccgcaa cgccgatgaa cacgccgccg aattttctcg gctggccgaa
     4321 cggcacggcc tcgtgatcgt cgcgccgctg ttcgacgaaa cgcgctttcc cgactaccaa
     4381 cgcctaggcc gcagcgggaa gggcgagcgt gccgacctga tgcttgatcg catcgttgcc
     4441 gaggtggccc aactcagcgg cacagacggg acgcgctttc atctgttcgg ctattcgggt
     4501 ggcggccagt tcgcgcaccg ctacgcactc gcccatccgg agcgcgtagt cgcctacgcc
     4561 attggtgccg ccggctggta cacgctgccc gacgcggcgc aaaaatatcc gctggggatt
     4621 cgctcgaccg aacgcctacc tgatctggct ttcgatccgc ggcgcttcct cgctattccg
     4681 gccgcggtat tcgtcggcga tcgcgacgtc catgacggaa cggcattgcg tcaaagcgag
     4741 cgtctgcgtg ccgagcaggg agaaaaccga cacgaacgtg ggcggacatg gatcgacgcg
     4801 atgacgcacg ccgcccgctc gctggcgatg gagaccgagt tccatttcga gtcgctgccg
     4861 cgcagccccc actgctttct caagagcatg cgtcgcggcg ggctcggcga gcgcgtggtg
     4921 gcatggatgt tcgatcgtcc acaggaggtt cgcccaccgt tgtgtggcgc cgtcaccctg
     4981 tcgttggtac cggcgttcac ggccgaacgc atcgcaccca aggcaattct ttcgtgcaaa
     5041 gcaaacggcg acccggctac aggcgccacg atgcgcgcct ctcctgcggg cgcttcccga
     5101 atcagaactt gtgccgcagg cccaccatga ctgccgcgac ggcctcgccc ggttgcggat
     5161 cgaagttcca ccgatatttg gcgccgacct tgttggctaa gcgtgaatac gctaggtaca
     5221 tcgttgtctc gcggtcgagt gcgcgcaagt aatacagcga agccccactg acgtcggtcg
     5281 ccgccggctc gccgctttcg tcgcgacgcg ccacgctcaa tgcgagcgtg ttcttgccga
     5341 ggatgcgaaa ccgaacgccg accgaggcag tacgcgcctc gccggttccg gcgctacccg
     5401 attcgcggtc gcgcgtagct tgggcaaaca gcttcaccgg cccgaaatcg agcgccaccc
     5461 ccccgccccg cgtggttgcc aggccacccg tgtcgttgac gaaacgtcgc tgcgtgaccg
     5521 ccgcgacact caggatctca ccatcgaaga ctgctgcgac acccgaaccc gaaccctcct
     5581 tgcgcacctg gctttcagcg ctggcgttct cgtcgccgac ccagtaccca gcatcaataa
     5641 agaagccgcc ccacgacggc gtacggtact tgaccatgtc gtcgaaacgc acgttgtaac
     5701 cgcgccgcac tgagtcactc gtccggtgca ggatgcctac gctgcgcgcg gcacctcctg
     5761 ttgcctcgag cggatcgagc gcaacgaaag ccggcgagta ttgacggccg ccggtgacgc
     5821 gtccaaacgc cccctccacc cccgcgtagg cttgccggcc gaacaggcga tcgtcctgtt
     5881 cggattggcc ggtacccgga tcgaatccgg cctcgagaac aaacacgccg gtgaagtcat
     5941 cactgaacgc ggccttccct ttgacgccaa tgcgcgagcc gtcgagttcg ccgccttcga
     6001 aaccgatccg cttgaccgca ccggcctttt cgacgccgag actgacatcg accacgccat
     6061 agaactcgat tgacttttcg aaagccgacg cgggcacggg cgccgacacc gccagaaaag
     6121 cgagcgcaat acccgccatt cccatcactt tccgcatcgt cttctccctt gtatgctggt
     6181 cgattccgtt ggatctgtcg gccgggcgaa cgcgcgccgg caaccggccc ctagaaggtc
     6241 gcggccggct aatcattcgc ggacgcttat cgtcagccag accatcggcg acgtcaggtt
     6301 gaccggcgtc gtgccgtgta gcgcctgctc ggagcggtcg gcgatctcct gatggcgagg
     6361 accgatgttg attccggcaa gggcgatctc gaccgtcggc tccagcgcgt aggcgaggcg
     6421 aaagtcgacc gtgtggtaac ccggcgatgc gtactcggtc gtcggcacga agcccgaccc
     6481 cccggcgatc gagcccatgc gcaggatgct cagcgcgccc gaccagcgtc ggtggttctg
     6541 gaagtaactg aggcttgccg tatgcgcggc ggtcagacga cggaccagcg tgtcatcgct
     6601 gcggcggtcg atcagcgcgt ggctgaagag gaattgcccg cccgcccacg ggcggtggtc
     6661 gatctggtac tcgacaccgc gcagctcgac cggcgttgcg agattctcgt agcgcgccgt
     6721 cggcagaccg agcaggccgg cgctgggcag cgcggcgcgg gcgatgtaac cgtcgattcg
     6781 ctcgcggaag aggcggacgt cgacgcgggc atcccaatcc gggatctcgc cgaggtagcc
     6841 gatttcgatg ctgtccattc gcgatggctg cagcgcgatg ttcggcgcgt agggttgcgc
     6901 cagcagacca aacccgggtt gggtcacctg cacgtcaccg cgccgctcaa agatgaacgg
     6961 gccgcgccag gcgcgcgcgt agccggcccg ccaggcatcg tgccggctgg cctgccagtt
     7021 cacgaaaaat cgcggcgaaa acttcttttc gtggtcgtcg aaacgctcga ccaggcctga
     7081 cgccgtggtc gtcaccgatg gcgtcagccg gttttcgatc tgcccgaaca ggcggcccag
     7141 atgctggtgc tggtcgggct ggccggcgta gagaaagggc gcggtgatcg cgtcacggcg
     7201 caactcgccg ccccagaccg tccgcacggc gtccgaccag gacagccgat gctggaactc
     7261 gacgctgtcg cgctggcttt cacgattgcg gtcgagcgga acgccggtca ccagcggcgg
     7321 cgctgcggcc tgccaggcct cggtcacttc gtcgttgttg tggtagtaac tgaaactcca
     7381 ttcctcgatc gaccgcggcg tgtgtcgcca ctgcagcagg acatgggtgc tcgagaaatc
     7441 ggcctcgcgc tcgccgttgt tgttgaacag cgagtcggcg tagccgagct gctgctggcc
     7501 gcgactggca ccgagcagtg cggtcagctc atcgcggttg cccggccgat agtcgccgcg
     7561 cagcgagacg atgctggtgc ggtgactgtc gtgcaggtca tcgaagccgt cgtcgccgcg
     7621 gaagctggca ttcaggcgca cgctgccgcg ttcgccggcg ccacgccact gcgccgcccc
     7681 accgcgccgg ccctgattac cggcgctcac cgaggcagcc gaaccgtcaa gatcaccact
     7741 gtgccgggtg atgatgttga ccacgccgag cacggcattc gagccgtacg ccgtcgaatt
     7801 ggtcccgcgc acgacctcga tgcgctcgat atcgtcgacc gtcaccggca acgaactcca
     7861 gtcaacgccg tcgaagttcg ccggcgagaa aaccgagcgc ccgtcgacga gcacctgcat
     7921 ccacgacgga tagtcgttgc ccatcccgtg gtaggtcacc cagtgcttgc caccgcgctc
     7981 ctgcccgacc tgcatgccgg gaacgagtcg cagcagtcgc ggaatgtcct catagccggt
     8041 tgcgcgaatc atctcgcggt cgatgacggt caccgcggcc ggcgcttcat tctgcggctg
     8101 cggcagacgg gaaggcgtga ggacgatggg caggtcgacg aagtagctgg cttcgatccg
     8161 ttcagcgtac gccgcggcag gggccagcaa gccaaggagc aggcaggcga cggccttcgc
     8221 cctcggcaat atttcaagtc cttgcatccc aacgccccgg attattcacg tcatttttgt
     8281 tggcggcagg ataaccgaag ccggcctaaa ccgcgggcga gacacgcagc aggcggacac
     8341 caggcgcccg gcgctacgct ggcgccgtga gttgagccat ttcgccctca gtcaaccatc
     8401 gccattgccc tctcggcagg tcgccgggca aggacagccc gccgacggcg aggcgttcaa
     8461 gggcttcaac ccggttgccg gcggcggcga tcatgcgctt gacctggtgg taacgccctt
     8521 cctgaatcgt cagcaacagc cgtcgtggcg attcgatggc gacctcgctg gccgccacgg
     8581 cagcgggcga atcacgaagg acgacaccgg ccatcagccg ttcgccgaga tccgcatcgg
     8641 cgtcgtgctt gagcgtcact gcatagcgct tggcggcgcc gtgccggggc gaggtcagcc
     8701 gatggttgag cgcgccgtcg tcagtgacga ttagcaggcc ggcggtgtcg gcatcgagcc
     8761 ggccgaccgg ctgcaggccg cggcgcgcca gttgcggcgg cagcagggtc agcaccgaag
     8821 ggtggtgggt cggcgagcgc gaacactcga aaccggtcgg cttgttcagc atcagcacgg
     8881 catgctcgcg gcacacccaa tcgacgccat cgacgcgcac cgccacgcca tcgctggcga
     8941 acggcgcgtc ggggttgtcg caggcagcgc cctcgacggc caccagaccg gcattcacca
     9001 gtcgccggca atcgcgccgg ctgccgaagc cttcgcgctg caacaacctg tcgagcgaaa
     9061 tcatgaggct ttcgccagcg tcttcagcgt cttgccgatc gccgcgaatt cggcgctctt
     9121 ggtcgacgtc ggcctggcaa ccagcgcgat accgcgcacc ggcggcggct tgagcttgcg
     9181 tacgacgagt cccgccgact cgattagacc gctgccgatc gccatctgcg gcatcagcgc
     9241 gataccgagt ccgcccgcca ccatctgcac cagcgtgatc aggctcgacg cctcgattcc
     9301 cctgcgctgg gtcgccgcct gaccggccga gcaagccacc agcgtgtgct cgcgcaggca
     9361 gtgaccctcc tccagcagga tcaggctgcc ggcatcgagc gattccggag cgatcggcgg
     9421 ccgcggcatc ggcgcgccga cggcgctgac cacgaccagc ggatcgtcgt agagctgctc
     9481 gacatgcagg tcgtcgagcg gaaacggcag cgcgagcaca gcgaaatcga gcgagccatc
     9541 gcgcagctgg tcggcgagcc gggcgctggt gtcctcgcgc agcatcagcc gcaactcggg
     9601 cgccgactcg cgcaggctcg caacaaggcc gggcagcacg aagggcgcaa tggtcggaat
     9661 cgcgccgaaa tgcagcacgc cagtcatcgg cctggcggcg gtggttgcca acgccacgag
     9721 atcctcggtt tcggcaagca ggcggcgggc ccgcgccacc aaggactcgc cgatcgctgt
     9781 cagaagcacc cgcgagcggt cgcgctcgaa aagcttgacg ccgaggccgt tctccagctc
     9841 cttgatgccg gccgacaacg agggctgcga gacacagcag gcttcggcgg cgtgggtgaa
     9901 gctgagacag tcggcgaccg caacgaggta gcgcaactgc cgtggcgacg gcagaaaact
     9961 ggttcgtgcg gcaggctgcg gcggctgggc tgggcgcttc tctggcattg gctttctcaa
    10021 tcaggtttgc cgcaattata ggagttcttg ctccccgatg accgtgtcga ggttcgagcg
    10081 gggcgaatca gcccgtccgg agaaaccatt tttgcagtgc gccaattatt tgacgcgaat
    10141 caaggttcgg caccaatccg gggcgtagcg tggaatcgtt cctccgaacc agcggaattt
    10201 cgcccatgct gacccgtcgt caactgctac gcggtgattt cttggccaag gcctacagcc
    10261 tcgcgccgcc gtgggcagtg cggcatttca ccagcgcctg cagccgctgc gacgcctgcg
    10321 tcacagcctg cccgactggc ctgctaatcc gcggcgacgg cggctttccg aggaccgact
    10381 acagccgcag cgaatgcacc ttctgtggcg attgcgccac cgcctgcaag gacgacgcgc
    10441 tgcggcgcgg cgacggccag gcgccgtggt cgcgccgcat cctaatcgac gacagctgcc
    10501 tgacgcgcat cggcgtcgtc tgccgcagtt gcggcgagca ctgcgacgcg caggccatcc
    10561 gcttcgtggc gcaggccggc ggcgtcgcca atccctcact cgatgccgca gcctgcacgg
    10621 gctgcggcgc ctgcgtcgcg gtctgtccga accgggcaat ccgcgtcgtc gccaacactg
    10681 ccgaattttc caggagagct gcatgaatat ttccagcgtc gtcctgcgcg ctcagccggc
    10741 gcgcattccc gaaatccgcg acgagattgc ccgccgcccg ggacatgaga tccacggcga
    10801 gcacgaagcg gggcgcctgg cgctgaccat cgaggacagc gatacccgct cggcggccga
    10861 tgcctacgtc gccctgcacg acattcccgg cgtcatcgcc gtatcgctga tctaccagta
    10921 ctccgacgac gagacggcaa ctgccgaacc cgccgccgaa accattgcga attgaaagag
    10981 gaggcaagac catgaaactc acccgtcgcg atttcatcaa gacccaggcc gccgccgccg
    11041 ccgttgctgc cgccggcctg cctgccacct cggtcgtcgc cgccgacaag gccgacgaca
    11101 tccgctggga caaggcgccc tgccgcttct gcggcaccgg ctgctcggtg ctggtcggaa
    11161 ccaaggacgg ccgcgtcgtc gccacgcaag gcgaccctga cgcgccggtc aaccggggcc
    11221 tcaactgcat caagggctac ttcctatcga agatccagta cggcgccgac cgcctgacca
    11281 gcccgatgct gcgcaaaaag aacggcaagt acgacaaaaa cggcgaattc acgccggtgt
    11341 cgtgggatga ggccttcgac atcatggctg aaaagtggaa ggcaacgctg aaggccaagg
    11401 gcccgaccgg cgtcgccatg ttcggttcgg gccaatggac ggtctgggag ggatacgccg
    11461 ccgccaagct gttcaaggcc ggcttccgct ccaacaacat cgatcccaac gcccgccact
    11521 gcatggccag cgctgttgcc ggcttcatgc gcaccttcgg catcgacgag ccgatgggct
    11581 gttacgacga cctcgagaac gccgatgcgt ttgttctgtg gggctcgaac atggccgaga
    11641 tgcacccgat cctctggtcg cgcctgaccg accgcaagct ctccgccaag cacgtcaagg
    11701 tacatgtgct gtcgaccttc gagcaccgca gcttcgaact ggccgacaac gcgatgatct
    11761 tcaagccgca gaccgacctg gcgatcctca actacatctg caaccacatc atcaagagcg
    11821 gcaaggtcaa caaggacttc gttgccaagc acgtgaactt caagaagggc gaaaccgaca
    11881 tcggattcgg cctgcgtccg aaccacgcgc tcgagaaggt cgccaccaac aacggctacc
    11941 cgggcgagga cagcaagccc aagggcaatc cgggcaaggc cgatccgatc accttcgagg
    12001 aattcgccaa gttcgtctct gagtacacgg tcgagaaggc ctcgaagatc tccggcgtgc
    12061 cggtcgagaa tctcaagcgt ctggccgagc tctacgccga cccgaaggcg aagatcacct
    12121 cgtactggac gatgggcttc aaccagcaca cgcgcggcac ctgggtgaac aacatgatct
    12181 acaacgttca cctgctgacc ggcaagatct ccgagcccgg caacggcccg ttctcgctga
    12241 ccggccagcc atcggcctgc ggcaccgccc gcgaagtcgg caccttcgcc caccggctgc
    12301 ccgccgacat ggttgtgatg aatcccgagc atcgcgccaa atcggaaaaa atctggcagc
    12361 tgcccgaagg cacgatcccc gacaagcccg gcacgcacgc cgtactgatg gcgcgcgcgc
    12421 tcaaggacaa gaaggtcaac gtctactggc agatgtgcaa caacaacatg caggccggtc
    12481 cgaacatcaa tgacgaactg ttcccgggct ggcgtgatcc ggagaacttc atcgtcgttt
    12541 ccgatccgta tccgacggtc tcggcgatgg ctgccgacct cgtcctgccg accgcgatgt
    12601 gggtcgagaa ggaaggcgcc tacggcaacg ccgaacggcg aacgcagttc tggcgccagc
    12661 aggtgaaggg gccgggccag tcgcagtccg atctgtggca ggtcgtcgaa ttctcgaaac
    12721 gcttcaagat ggaagaggtc tggcccgccg aactgctcgc caaggcgccg cagtacaagg
    12781 gcaagacgct ctacgacgtg ctcttcagga acggcgtggt gaacaagttc ccggttgcaa
    12841 actgcacgca ggtcaacggc aacgtgatcg ccaattacgt caacgacgag tcaaagcact
    12901 tcggctatta cctgcagaag gggctgttcg aggagtacgc cgctttcggt cgcggcaagg
    12961 cgcacgacct tgccgacttc gacacctacc accatgcgcg cggcctgcgc tggccggtgg
    13021 tcgacggcaa ggaaacgctg tggcgcttcc gcgaaggcta cgacccctac gtggccaagg
    13081 gcgaggacgt gaagttctac ggccacaagg acggcaaggc ggtgatcttc gcgctgccct
    13141 atcaggaccc gccggagatg ccggacaagg acttcgacct ctggctgtgc accggccgcg
    13201 tgctcgagca ctggcacacc ggcacgatga cgcggcgcgt ccccgaactg caccgctcct
    13261 tccccgaagc cgtgctgttc atgcaccccg acgacgccaa ggaacgcggc ctgcagcgcg
    13321 gcatggtggc gaagatcgcc tcgcgccgcg gcgagatcga gctctacgtc gagacgcgcg
    13381 gtcgcaacaa gccaccgaag ggactggtct tcgtgccctt cttcgacgcc gggcgcctga
    13441 tcaacaaact gacgctcgac gcgacctgcc cgatctcgaa agagaccgac ttcaagaagt
    13501 gcgcggtcaa ggtcgtcaag gcctgagcgg atagccagca cccgagcaca tcatggtcag
    13561 cgaaacccgc cgccccgcct cgcccgaccg ccggcagttc atcgccggca gtctcggcgc
    13621 gcttggcggc gcgacgctgt tcgggctcgg ggttggcctg gttgcacgcg aagcgggggc
    13681 actgcctgcc gacgccatcc gcccgccggg cgcgttgtcc gaggaccgtt tttcgtcagc
    13741 ctgtgtacgt tgtggcctgt gcgttcgcga ctgtccctac gacacgctga agctcggcgt
    13801 gatcggcgac ggcgtcgggc cgggtacgcc cttcttcaac gcgcgggcga ttccctgcga
    13861 gatgtgcgag gacatcccct gcgtcaaggc ctgccccacc ggcgcgctcg atccggcgct
    13921 gaaggacatc gacaattcgc gcatgggcct cgcggtgctc gccgatcagg aaacctgtct
    13981 caattttctc gggctgcgct gcgacgtctg ctaccgcgtc tgtccgttga tcgacacggc
    14041 aatcaccctg gaaacccggc gcaatccgcg ctccgatcgc cacgcgctgc tgatcccgac
    14101 cgtgcattcg gatgcctgca ccggctgcgg caaatgcgag cacgcctgcg tgctcgaaga
    14161 agcggcgatc aagatcttcc cgatcgcctt ggccaagggc aagctcggca gccactaccg
    14221 caagggctgg gaggaaaagg ccaaggccgg cggctcgctg ctcgaggaca tgccggcgcc
    14281 ggaagcccgc cgccccgacg cgccgcgcgt cgatccgcaa cttgaaacgc tcggtggagg
    14341 cctgcgatga gtttcatcgt tgccgccacc gacatgccgg gccgcacggc gagcgccgcg
    14401 cgcggctggc tcgccgccaa ccgctacctg ctgatgcgcc gtgcctcgca gctcgcggtg
    14461 ttggcgctgt tcctgctcgg tccctgggcc ggcatctggc tgatcaaggg caacctctcg
    14521 gcgagcctgc tactcgacac ggtgccgatg accgacccgc tgctgatggc gcaggcgctc
    14581 gccgcccggc actggcccga agcaacggcc tggatcggcg ccggtgtcgt cgtcggcttc
    14641 tacctgctgg tcggcggccg cagcttctgc gcctgggtct gcccgctcaa catcgtcacc
    14701 gacacctcgg cctggctgcg ccggcgcctg ggcctcaagg gcggcaccgc accctcgcgc
    14761 cggctgcgcc actggctgct gctggcgctg ctgatcgccg ccttcgcctc gggccgcatg
    14821 gtctgggagt gggtgaatcc ggtatcgatg acccagcgcg cgctgatctt cggcggcgcc
    14881 ggcggtctcg cggtggcact cggcatcttc ctctacgacc tgctcgtcgc gccgcgcggc
    14941 tggtgcggcc acgtatgccc gaccggcgcc gcctacgcgc tgatcggccg cgcctctctg
    15001 acccgtgttg ccgcagcgcg ccgcgagcgc tgcgacgact gcctcgactg ctttgccgtc
    15061 tgccccgaac cgcaggtgat tcgcccggcc ctcaagggca ccggcagccc gctgattctt
    15121 gacagcgatt gcaccaattg tggccgctgc atcgacgtct gtgcgccgtc ggtgtttcgg
    15181 ctgacccatc gattcgatac ccggagggac gcatcatgaa aacactgatc aaccgtaagg
    15241 ccaccatcct cttcgccgcc tgccttgcgt tcgcgatgcc gatctcggcg caggagaagc
    15301 tcgtgtccga gcgcggcgac gtgccaatca acgcggaatc caagcccgag ccgtacaaga
    15361 tcgaacgcga ccgcgacccg attccgcgcg actacgtgca gcagccgccg ctgatcccgc
    15421 actcgaccaa gggctactcg atcacgcagg acttcaacaa gtgcctcgat tgccactcgt
    15481 ggacgcgcta caaggaattc aacgcgacca aggtcagcct gacccacttc aaggaccgtg
    15541 acggcaaaga gctcgccaac gtctcgccgc ggcgctactt ctgcctgcag tgccacgtcg
    15601 tccagttcga cgccaagccg ctcgtcgaaa accagttccg gcccgtcgcc ggcgtgcgct
    15661 gagcggcgcg gaccggggag aacgcgatga tcaaagaaat tcttaataag gtaaaggcgc
    15721 tcggctggcc gctcgccgcc ggcctcttca ccgccggcat catcttctgg ggcggcttca
    15781 actgggcgct cgaggcgacc aacaccgagc agttctgcat ctcctgccac gagatgcggg
    15841 agaacgtgtt taaggaatac aagaacaccg tgcactacac caaccgcact ggcgtccgcg
    15901 ccacctgccc cgattgccac gtaccgaagg aatgggtcta taagatcaag cgcaagatcc
    15961 aggcgtccaa cgaggtctgg cacaaactgc tcggctcgat cgacacgccc gagaaattcc
    16021 acgccaagcg cgcacagcta gccgaaaacg aatggcggcg gatgcgcgcc accgactcgc
    16081 gcgagtgccg caactgccac gacttcgaat tcatggacta cacggagcaa ggacgccgcg
    16141 ctggcaaggc gcacccagaa ggcttcgccg aaggcaagac ctgcatcgac tgccacaagg
    16201 gcattgctca cgcgctgccg ccgatcgacc aggacatcgg tgaccgcaac atcggcgcca
    16261 ccaagatctc gcacggcgaa acggcgaaag acaagggcta ctgaaaaacc cacgaaggcg
    16321 aacagcaaga cgccggctga tgccggcgtt ttcttctaca cccagtgcgg agcacctggt
    16381 actcaatggc caattcaaag ctctgtcaat aaagtgacgc cgactccgaa aacggctccg
    16441 agcggcggcg cccaatgccg ccgcaacctg gattgaggcg caatctcttg aaacattaga
    16501 tacacaattc caccagctaa accggccgga caccatgcaa gctggactga agctgcattg
    16561 cggcgatgcc tcgggcatct tgccgccgcg agaccgattg ctggatacaa atagtgaggt
    16621 ggccgtcgga atgtcttggg tgaaggtcca ctactggccg acccctgcca ccaaatacat
    16681 ccaccccact caggaacccg gactgcgcat cgaggcgata aagcggtcgg cttcggcgat
    16741 agccttgttc atgtcggtga tcagtgcgcc gatgtcagct tcgacggtgg cgcgctggcc
    16801 gcgcagcgac gcgatcgcac gggcgttaag gttgtgcttg agaaaaagca cgcgatcctt
    16861 gaaggcggcg agtactgggt cgagcttctt ctcggcggcg cgcatggcgc caatcagctg
    16921 ccggtaacga cccttggtgt cctgcagctg cttctggctc gccagccgca attcggcgct
    16981 ctgatattcg ctaagttcct tctcccactc ggcgaagagg tcggtcgcca cgcgctcgac
    17041 ctcggcaatt tccttacgca cgtcgttggc gcgcgcttcg ctgtcttcgt attcgtcctt
    17101 cagcgtgttg tactgctttt cgagatcgcc gccgtcgaaa ccggtaacgg cgacgaactg
    17161 ctcgagcgcc gaggcaaact gttttttcgc tgcttcctgc gcctcgcgtg catcctcgac
    17221 gttgcccacc aacaggtcgc gcttttcgta gccgagcttt tccatcgcgc cgtaataggc
    17281 ggtctggcaa ccggccagtg ccagcaggcc gagtaccagc gctgtacggc cgatgactcc
    17341 tcgtcttgtt gtcatgttca tctccggtgg attctcgtcg cagccatggc ttgtggatgg
    17401 catatcgtgc cgccaagaat acctgctgtc cccgcgcctg caagccggga ttgccgacgg
    17461 gcggcgaatt cgcgccggtg gtgaatcagc gttcgtcgcc gatcgcccac tcggcggcac
    17521 tgcgcgcgtg gatcgccgcg gtgtcgaaca tcggcaccgg cgaatcatcg gcgccgacaa
    17581 gcagcgcgat ctcggtgcag ccgagaatga tcccctgcgc accacgcccg accagccgcg
    17641 cgatcaccgc ccggtaggcg gcgcgcgagt tgtcgagcag tcggccctgg catagctcgt
    17701 cgtagatgac ccggtgcacc agctcccggt cgggggcatc ggggacgacg acgccgaggc
    17761 cgtgccgctg ttgcaggcgc gcccggtaaa aatcctcttc catcgtgaaa cgcgtgccaa
    17821 gcaggccaac ggttcgcagg ccgtgctcgg caatggtcgc tgcggtgggg tcggcaatgt
    17881 gcagcagggg tacgcgcagc gctgcggcga gcggatcggc gagcttgtgc atggtattgg
    17941 tgcagagcac gacggcgtcc gcgccggctg tcaccagcgt ctgcccggcc tctacgagca
    18001 ggcgcccggc ggcctcccac tccccggcgc gctggagtgc ttcgacctcg tggaaatcga
    18061 tgctgaccag gacgatcttc gccgagtgca gtccgccaag gcgatcccgc accgtctcgt
    18121 taatgatccg gtaatacggc agcgtcgact cccaactcat gccgccgatc agtccgatgg
    18181 tcctcatgga agcggctccc gttccgtcgt gtcgccagtt tgccggaagc gcggccttcc
    18241 ggtgcgcgcc cgttctccta aatacagagc gctccggaat taggcggctg tgtgctacgc
    18301 ccggacggcg gcggtggatt aaccccgcca gcgatcagcc ggcggcgcga tccgcggcac
    18361 agtccgcggt gaccgacttc cagttggcgg cgaacgattc gccctgcgtt gcggtcgaag
    18421 tgctgcacgt gacgaccgga atcggaatcc ggccggtatg atccgcgttc ttctttttga
    18481 actcccgctt acaggaatcc gccatgctca agcccttatc gctgtctgtc cttgccctga
    18541 tcgtcggcct aggcttcgcc ccgagcgacg ccgaagccaa gcgcctcggc ggcgcgcgtt
    18601 cgttcggcat gcagcggcag gcgacacccc cggccaaggc ccccgcggct gcgccggccc
    18661 cgacgcaggc cccggctggt gccccggccg cccaaccgaa gcgctcgtgg atgggtccga
    18721 ttgccggcct cgccgccggc cttggtcttg ccgcgctggc ctcgcaccta ggcctcggcg
    18781 aggagctggc gtcgatgctg ctgatcggcc tgctgatcgt cggcggcatc atcgtattcc
    18841 gcctgctcac gcgccgcgct tcaccggcac cgcgtccggc gatggccggc gcctcgccgt
    18901 atgcggcaca tgagccgccg gctgcgccca ccgagcgtgc tccgctggcc ggcagccatg
    18961 gtcaggcgat gcccggttcg gttgccgact cgtttgccaa acccgacagt gccgcgccac
    19021 ggattccggc cgactttgac gtcgtcgcct tctcgcgcaa cgccaaggtg aatttcgtcc
    19081 gcctgcaggc cgccaacgat gccggcgacc tcgacgatct gcgcgccttt accacgcccg
    19141 agatgttcgc cgaactgaag atggacatcg ccgagcgagg cagcgccccg cagcacaccg
    19201 acgtggtcga gatcgacgcc gaagtgatcg aggcagtcga agaaggttcg cgctacgtgg
    19261 taagcgttcg cttccgcgga cagctccgcg aagaggagga tggcccgcta gtcacaatcg
    19321 acgagctgtg gcatctggtg aagccgcgcg aaggcaaggc cggctggctg ctcgcgggaa
    19381 tccagcagac gcagtaagcg gcaccgaccc ggccgccccg ccggccgccg ggaacgttcc
    19441 cgtccgctgg gcgtcgcacc ggaacccggc cggccttgcc ggactcggga gatcgcggat
    19501 gcccttcggc cgattgagtt ggtggccatg cctgtttgtt ctaacggcca tggcgctggc
    19561 cgcctgcggt gggcgcgacg actacggatc gagcagcaac ccgccgccgg ccggcagcgc
    19621 accgggcacg atcacgctgg cgaccaccgg actggccacc ggcagcagcg gcgcgctggc
    19681 catcaccggc cccggcggat tcgtcgcaaa catcccgacc acgcagacca gcgttgccgt
    19741 gcccggcccc ggcgactaca cgatcaccgc tgccgaggta ctgaacggca gccgcgtgct
    19801 ggctccccag gccgcgacac agacaatcac ggtgcgctcg ggcacgacgg ctgccgccac
    19861 cgtcgcctat cgcgacgacc gagcgctggc gctctcgctg cagcaagtgg tcaccagcct
    19921 cgtctcgccg ctctacctca cgtcaccgac aggcgacgca cgactgttcg tcgtcgagca
    19981 ggccggaacg atccgcatca tcagcggcgg cgtgctgctg ccgacgccct tcctcagcat
    20041 ccccaatccc agtcgcctgc tcgccggcgg cgagcgcggc ctgctgtcga tggccttcga
    20101 cccgcagtac gcagccaacg gcttcttcta cgtctacctc accgatcaaa acggcgacat
    20161 cgcgatcgag cgctatcagg tctccgccag caacgccaac agcgccgacc cgacgccgct
    20221 gcgcctgctc accatttccc accgggactt tgccaaccac aacggcggcc tgctcgcctt
    20281 cgggcccgac ggcatgctct acgccgctac cggtgacggc ggtggtggcg gtgacccgct
    20341 gggcagcggc cagaacctcg gttcgctgct cggaaagctg ctgcgcatcg acgtgcgcaa
    20401 tgccagttcg ggcacgctct acgtcgttcc gcccgacaac cccttcgtcg cgcaagcggg
    20461 cgcccgcccg gaaacctggg cctacggcct gcgcaatccc tggcgcttcg ctttcgatcc
    20521 gcctgccgga cgcctctaca tcgccgacgt cgggcagaac cgcatcgagg aaatcaacgc
    20581 cgtagccgcc gcgacggcgg gcctcaacta cggctggaac ttgacatcct ccccgccctc
    20641 ggcctgacgg c
//
LOCUS       DATDAA010000004         6990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_70002,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000004 DATDAA010000000
VERSION     DATDAA010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70002"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="VIS73_00165"
     CDS             <1..1240
                     /locus_tag="VIS73_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY5761600.1"
                     /translation="THGLFGALPSSARRGGELAAIAGQPPEPGAWPSGCRFAPRCPHR
                     QARCDAEAPGWTEVAPGHAVRCHFAGSLAGAGMAAASTATAPEPVTEPAREVLAVADL
                     AVHFPIRRGLLQRTVGHVRAVDGVSFSLSAGRTLALVGESGCGKTTVGKAVMRLIRAT
                     GGTICLNGEAVPAEGRVALQPLRRTAQMVFQDPFASLNPKRSVGEIIGEGIEALAIAP
                     AESRARLIAEVLDSVGLPRDTATRYPHEFSGGQRQRIAIARALAVRPQVLICDEPTSA
                     LDVSVQAQVLNLLRRLQREQGLAYLFITHNFGVVEFLADQVAVMYLGRIVEQGDTPTL
                     LKAAAHPYTRALLDAVPSVDGAAVPPRLAGEMPSPAKPPSGCHFHPRCPHAADICRQQ
                     YPDERQLASGQRVRCHFPLA"
     gene            complement(1301..2818)
                     /locus_tag="VIS73_00170"
     CDS             complement(1301..2818)
                     /locus_tag="VIS73_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY5761601.1"
                     /translation="MTTTTQEFPHSAAAPKAQRLAFAIAASVLLGGCASLKLDSTASA
                     EDRPPSAAIVSKPAPVAPAPPLAAKAPETPQATTPAARDPQQISEVATDPGPPIQATP
                     PVAVNPEPVIEPPADLWARIESGFAIADIEHPLVDRHMKWYLDRPDYMRRMIERSQLY
                     LYFVVEELNRRNLPTELALLPMIESAYNPMAHSRARAAGMWQFMPATGKHYGLEQNWW
                     ADERRDVVSSTRAALDYLERIYVMHGDWHLSLASYNWGEGAVKRAVEKNRRKGLPTTY
                     THIKMPAETRGYVPKLQAIKNILGNKALREKLALPSIPDEPYFVAIERPEEMDVRLAA
                     EFAEISLEEFVALNPAHNRPILRTDVPLLLPRDHVDRFLARVARHDEPFISWQKYVFQ
                     KKDRIDKVAKRFGLTEQQLRLANGLTGKRRNVKPGQQLMVPLPDKATAETLSVVAALP
                     PFSITDPPPKKKRRYYRKRAKLAALVPAPGEVKPVKKKKAAARRPLKKKTGNTKG"
     gene            complement(2924..3000)
                     /locus_tag="VIS73_00175"
     tRNA            complement(2924..3000)
                     /locus_tag="VIS73_00175"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2964..2966),aa:Asp,seq:gtc)
     gene            complement(3048..3123)
                     /locus_tag="VIS73_00180"
     tRNA            complement(3048..3123)
                     /locus_tag="VIS73_00180"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3087..3089),aa:Glu,seq:ttc)
     gene            complement(3149..3224)
                     /locus_tag="VIS73_00185"
     tRNA            complement(3149..3224)
                     /locus_tag="VIS73_00185"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3189..3191),aa:Ala,seq:ggc)
     gene            complement(3317..4720)
                     /gene="gltX"
                     /locus_tag="VIS73_00190"
     CDS             complement(3317..4720)
                     /gene="gltX"
                     /locus_tag="VIS73_00190"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY5761602.1"
                     /translation="MVRTRFAPSPTGYLHIGGARTALFCWAYARRHGGTFILRVEDTD
                     VARSTPEAVQAILDGMAWLGLDADEGPFYQMQRMDRYREVIAQMLAAGTAYHCYMSAA
                     ELDALREAQKARGEKPRYDGTWRPEPGKTLPQPPAGVSPVVRFRNPEGGAVSWDDQVK
                     GRIEFANAELDDLVIARADGTPTYNFCVCVDDWDMRITHVIRGDDHVNNTPRQINILR
                     ALGAEVPAYAHLSMILGDDGQKLSKRHGAVSVMQYSDDGFLPEAVINYLARLGWSHGD
                     DEIFTREQLVSWFDLDHITPSAAQFNTEKLLWLNQHYLKQASADSLVAPVGARLRAAG
                     LDGAAGPSLVAAIALYQDRCNTLNLLAESVSVFYRDVVADDETLAPYRTPECRAALGA
                     FVDAVAGTAWQREALSATIKAVVKAQGVKMPVLAMPLRIALTGQAQAPSVDALLELSG
                     EQLATTRVKALLARLST"
     gene            4828..4902
                     /locus_tag="VIS73_00195"
     tRNA            4828..4902
                     /locus_tag="VIS73_00195"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4860..4862,aa:Val,seq:cac)
     gene            5003..5824
                     /locus_tag="VIS73_00200"
     CDS             5003..5824
                     /locus_tag="VIS73_00200"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5761603.1"
                     /translation="MTCFRLARALARQVVLVASCVLPGVLWAADGSEDWLRNPSISNY
                     KAYAEFKMANYAEAKAIWQVLAERDNSEALFNLAILAEDGLGEAQDMSRALSLYQRSA
                     LAGGSKAQYRLGLLYSAGGPVPRDLGQARRFLSMAADGGDDDARRRLDALGEAGPATG
                     FARAETLSGQGRHAEAAAIYRELAGQGDAKARTRLAWLHEAGRGVPRDLAEAARLFRL
                     SAEAGEAEAQYALAVMLDTGRGQVQDRAESRRWLERSAQAGFSDAQQALRERSLR"
     gene            complement(5894..>6990)
                     /locus_tag="VIS73_00205"
     CDS             complement(5894..>6990)
                     /locus_tag="VIS73_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY5761604.1"
                     /translation="TLNVNGTVNAFYVNSNAKTTDGAGVTTSNKQANVQNGLLPGWIH
                     FAATTQQDGYDIKAHFGFAPGINSSSSIVGLPDGAGNAYSQTDNRNVYFQFGNASMGT
                     LKFGRDIGLFMQNPILNDMTLLGVGGTTRAAEPFNTSFGMIGHGYMYTGFQPQITYSM
                     PAAGGFNASFGLFNPSQYAGNQRKSPGIQGLASYEWKGGVNGKVWGSFVNQKTESDNP
                     ACVGNQQCGFKASGYEGGVKLGMGAFEVLVAGFSGKGIGISTVGAQFLGGLNANGDPM
                     KSDGMFVQGTVMAAEKLKLGVNYGKNTDKDLAAVGTESFNEAVSLGAYYNLTKSVTLV
                     GEVVNEEAGVDGAVAKTKTSTVSLGAILFF"
BASE COUNT         1095 a         2178 c         2429 g         1288 t
ORIGIN      
        1 tacccatggc ttgtttggcg ccttgccgag ttcggcaagg cgcggtggcg aacttgcagc
       61 gattgccggg cagcctcccg agccgggtgc ctggccgagc ggctgtcgct ttgcaccgcg
      121 ctgtccgcat cggcaggcac gctgcgatgc cgaggcgccg ggctggaccg aggtcgcgcc
      181 cgggcacgcc gtgcgttgcc acttcgccgg cagtctcgcc ggtgccggca tggccgctgc
      241 gtcgaccgcg acggcgccgg agccggtcac cgagccggcc cgcgaagtgc tggccgtcgc
      301 cgatctggcc gtgcatttcc cgatccgccg cggcctgctg cagcgaaccg tcggccacgt
      361 gcgcgccgtc gacggcgtgt ctttcagctt gtcggccggg cgcacgctgg cgctggtcgg
      421 cgaatcgggc tgtggcaaga ccacggtcgg caaggcggtg atgcgcctga tccgggcgac
      481 cggcggcacg atctgcctca acggcgaggc ggtgccggcc gaggggcgcg tcgcgctgca
      541 gcccctgcgg cgcacggcgc agatggtctt ccaggatccg ttcgcctcgc tcaatccgaa
      601 gcgctcggtg ggcgagatca ttggcgaggg aatcgaggcg ctggcgattg cgccggcgga
      661 atcccgagcg cgcctgatcg ccgaagtgct cgactcggtc ggcctgccgc gcgacacggc
      721 gacgcgctac ccgcatgaat tttccggcgg ccagaggcag cgcatcgcta tcgcgcgcgc
      781 gctcgcggtg cgcccacagg tgctgatctg cgacgaaccg acctcggcgc tcgacgtctc
      841 ggtacaggcg caggtactca acctgctgcg caggctgcag cgcgaacagg ggctggccta
      901 cctgttcatc acgcacaact tcggtgtcgt cgagtttctc gccgatcagg tggcggtgat
      961 gtatctcgga cgaatcgtcg agcagggcga cacgccgacg ctgctgaagg ccgccgcgca
     1021 tccctacacg cgggcgctgc tcgatgcggt acccagcgtt gacggcgctg ccgtaccacc
     1081 gcgcctcgcc ggcgaaatgc cgtcaccggc gaagccgcct tcgggttgcc atttccaccc
     1141 gcgctgccca cacgcggccg atatctgccg gcagcagtat cccgacgagc gccaactggc
     1201 gtcgggccag cgcgtgcgct gccattttcc gctcgcctga gggatctgct gcttgggaat
     1261 ggatgcccgg acgcgccgga cggcctggcg ctgaacgcgc tcagcccttg gtgttgccgg
     1321 tcttcttctt cagcgggcgg cgggcggcgg ccttcttctt cttgaccggc ttgacctcgc
     1381 cgggcgccgg caccagcgcc gccagcttgg cgcgcttgcg gtaatagcga cgcttcttct
     1441 tcggcggtgg gtcggtgatc gagaagggcg gcagcgcggc gacgaccgac agcgtttcgg
     1501 cagtcgcctt gtcgggcagc ggcaccatca gctgctgtcc gggcttgacg ttgcggcgct
     1561 taccggtcag gccgttggcc agccgcagct gctgctcggt gagaccgaag cgtttggcga
     1621 ccttgtcgat gcggtccttc ttctggaaga cgtatttctg ccagctgatg aagggctcgt
     1681 cgtgtctggc gacgcgcgcg aggaagcggt cgacatggtc gcgcggcagc agcagcggca
     1741 cgtcggtgcg caggatcggg cggttgtgtg cgggattgag cgcaacgaat tcctcaagcg
     1801 agatctcggc gaattcggcg gccagccgga cgtccatttc ttctgggcgt tcgatcgcca
     1861 cgaagtaggg ttcgtcgggt atcgagggca gcgccagctt ttcgcgcaaa gccttgttgc
     1921 cgaggatgtt cttgatcgcc tgcagcttcg gcacgtagcc gcgcgtctcg gcgggcatct
     1981 tgatgtgcgt gtatgtggtc ggcaggccct tgcggcggtt cttctcgacc gcgcgcttga
     2041 ccgcgccttc gccccagttg tacgaggcga gcgacaggtg ccagtcgccg tgcatgacgt
     2101 agatgcgctc aaggtagtcg agcgcggcgc gcgtcgagga gacaacatcg cggcgttcgt
     2161 cagcccacca gttctgttcg agaccgtagt gcttgccggt cgctggcatg aactgccaca
     2221 tgccggccgc gcgggcgcgc gaatgtgcca tcgggttgta ggcactctct atcatcggca
     2281 gcagcgccag ttcggtcggc aggttgcgcc gattcagttc ttcgacgacg aaatacaggt
     2341 agagctggct gcgctcgatc atgcggcgca tgtagtcagg gcgatcgagg taccacttca
     2401 tgtggcgatc gacgagcggg tgctcgatgt cggcgattgc gaatcccgat tcgattcgcg
     2461 cccagaggtc ggctggcggc tcgatcacgg gctcgggatt caccgcgacc ggcggcgtcg
     2521 cctggatcgg tgggcccgga tcggtcgcga cctcgctgat ctgctgcgga tcacgggcgg
     2581 cgggcgtggt ggcttgcgga gtctcgggcg ccttggccgc aagtggcgga gcgggtgcca
     2641 ccggtgccgg tttgctgacg atggctgcgg acggcggcct gtcttcggca gaagcggtcg
     2701 agtcgagctt cagcgaagca cagccaccga gcaggacgct ggccgcgatg gcgaaggcaa
     2761 gtcgttgggc tttcggcgca gcggccgagt gcggaaattc ctgggtggtc gtcgtcatca
     2821 gatggctacg gcggtcccga ggtgttggcg gccgaactaa atcggttcgc gcatttccaa
     2881 gagccgccgt gacgtgatgc aatgctcggc gacagagaag aactggcgga gtggacggga
     2941 ctcgaacccg cgacccccgg cgtgacaggc cggtattcta accaactgaa ctaccactcc
     3001 acacttgaat cgttggccgt agaccttgcg gttttcggcc agaaagctgg cgtccccacg
     3061 gggattcgaa ccccggttac cgccgtgaaa gggcgatgtc ctaggcctct agacgatggg
     3121 gacttcgtca catctctcgc agcttgactg gtggaggtaa gcgggatcga accgctgacc
     3181 tcttgcatgc catgcaagcg ctctcccagc tgagctatac ccccgccgcg aaagagcgcg
     3241 cattataggg atcgactttt cctttgtaaa gcgcgacagc gcgggaattt cgcagcgccg
     3301 ctttcgcgcc ggcgagctag gtcgaaagcc ttgccagcag ggcctttacc cgtgtcgtcg
     3361 cgagttgctc gccagacaat tcgagcaggg cgtcgaccga cggtgcctgt gcctggccgg
     3421 tcagcgcgat gcgcagcggc atcgccagaa ccggcatctt cacaccctgt gccttcacga
     3481 cggccttgat cgttgccgac aaggcctcgc gttgccacgc cgtgccggcg acggcgtcga
     3541 cgaaggcgcc aagtgcggcg cggcattcag gcgtgcgata aggcgcgagg gtctcgtcgt
     3601 cggcgacgac atcgcgatag aagacgctga ccgactcggc gagcaggttc agcgtgttgc
     3661 agcgatcctg gtagagcgcg atcgccgcga ccagcgaagg gcccgcggcg ccgtcaaggc
     3721 ctgcggcacg cagtcgcgcg ccaaccggcg cgaccagcga atcggcgctg gcctgcttga
     3781 ggtagtgctg gttaagccac agcagtttct cggtgttgaa ctgcgccgcc gagggcgtta
     3841 tgtggtcgag atcgaaccac gagacgagtt gctcccgcgt gaatatctcg tcgtcgccgt
     3901 gcgaccagcc gaggcgcgcg aggtagttga tcactgcctc gggcaggaag ccgtcgtcgg
     3961 aatactgcat aaccgatacc gcgccgtggc gcttggacaa cttctgaccg tcgtcgccga
     4021 ggatcatcga caggtgcgcg tacgcgggca cctcggcccc gagcgcacgc aggatgttga
     4081 tctgccgcgg cgtgttgttg acgtggtcat cgccgcggat cacatgggtg atccgcatgt
     4141 cccagtcgtc gacgcagacg cagaaattgt aggtcggcgt gccgtcggcg cgggcaatca
     4201 ccagatcgtc gagttcggcg ttggcgaact cgatgcggcc cttgacctgg tcgtcccagg
     4261 acaccgcgcc gccctcgggg ttgcggaagc gcaccacagg gctgacacct gctggaggct
     4321 gtggcagcgt cttgcccggc tcgggacgcc aggttccgtc gtaacgcggc ttctcgccac
     4381 gcgccttttg cgcctcgcgc aaggcgtcga gttcggctgc gctcatgtaa cagtggtatg
     4441 ccgttcccgc ggccagcatc tgcgcgatca cctcgcgata gcggtccatg cgctgcatct
     4501 ggtagaacgg cccttcgtcg gcgtcgaggc cgagccaggc catgccgtcg aggatcgcct
     4561 gcacagcctc gggcgtcgaa cgggcgacgt cggtgtcctc gacgcggagg atgaaggtgc
     4621 cgccgtggcg ccgggcgtag gcccagcaga aaagcgcggt gcgggcgccg ccgatatgca
     4681 ggtaaccggt cgggctggga gcgaagcggg tgcgaaccat ggaaagagcc gtgcgcaaaa
     4741 gctcggcatt ctaccgtggc ctccgctcgc atcctgacgc gaaattgacc gccgctcggc
     4801 gccaccgcta aaatgcgcgc ctctatcggg cggttagctc agcggtagag cactgccttc
     4861 acacggcagg ggtcactggt tcgatcccag taccgcccac caaatgcaag aaaatggcct
     4921 gcgtgagaac actcaggcca ttttcttgtc tgctcccttt gtgatcggtg tgattgcaga
     4981 ccgtttgtcg agaggagttc agatgacttg tttcagactg gcgcgcgccc ttgcgcgcca
     5041 agtcgttctt gttgcttcgt gcgtactgcc gggtgtgttg tgggctgccg atggttccga
     5101 ggattggctg cggaatccat cgatcagcaa ctacaaggcc tacgccgagt tcaagatggc
     5161 gaactacgcg gaggccaaag cgatctggca ggtgctggcc gagcgcgaca acagcgaggc
     5221 cctgttcaat ctcgcgatac ttgccgaaga cggccttggc gaagcgcagg acatgagccg
     5281 tgcattgtcg ctctaccaac gatcggcgct ggccggtggc agcaaggccc agtaccgcct
     5341 gggtctgctt tactcggccg gcggcccggt gcctcgagac ctggggcagg cgcgacgttt
     5401 tctctcgatg gcggccgacg gcggcgacga cgatgcgcgc cgtcgcctcg atgcgctcgg
     5461 cgaggcgggt ccggcgactg gtttcgcccg cgccgaaacg ctgagcggac agggccggca
     5521 tgccgaggcg gcagcgattt atcgcgagct ggcggggcag ggcgatgcca aggcgcggac
     5581 gcggctggcc tggctgcatg aggcgggccg cggcgtgccg cgcgatctgg ccgaagcggc
     5641 gcggctgttc cgcctgtcgg ccgaagccgg cgaagcagag gcgcagtacg cactggcggt
     5701 gatgctcgat accgggcggg gacaggtgca ggatcgggcc gaatcgcggc gctggctgga
     5761 gcgctcggcg caggctggat ttagcgacgc gcagcaggcg ttacgcgagc gttcgttgcg
     5821 ctaacgcgcg atcaaaaaag aaccgctcgg gacgccaagc gtccgcgagc ggttcaaacg
     5881 aggagactct tttctagaag aacaggatcg cgccgagcga gacggtgctg gtcttggtct
     5941 tggcgacggc gccatcgaca ccggcttctt cgttgacgac ctcgccgacc agcgtcacgc
     6001 tcttggtcag gttgtagtag gcgccgagcg aaacggcttc gttgaagctt tccgtgccca
     6061 ctgcggcaag atccttgtcg gtgttcttgc cgtagttgac gccgagcttg agtttctcgg
     6121 cggccatcac ggtgccctgc acgaacatgc cgtcgctctt catcgggtcg ccgttggcgt
     6181 tcaggccgcc gaggaactgt gcgccgaccg tcgagatgcc gatgcccttg cccgagaaac
     6241 cggccacgag aacctcgaag gcgcccatgc cgagcttgac gccaccctcg tagccgctgg
     6301 ccttgaagcc gcactgttgg ttgccgacgc aggccgggtt atcgctctcg gtcttctggt
     6361 tgacgaagga gccccagact ttgccgttga cgccaccctt ccactcgtag ctggccagac
     6421 cctggatgcc gggcgacttg cgctggttgc cggcgtactg gctcgggttg aagaggccga
     6481 aggaggcgtt gaagccgccg gcggccggca tgctgtaggt gatctgcggc tggaagccgg
     6541 tgtacatgta gccgtggccg atcatgccga agctggtgtt gaacggctcg gccgcgcgag
     6601 tcgtgccgcc gacgccgagc agcgtcatgt cgttgagaat cgggttttgc atgaacaggc
     6661 cgatgtcgcg gccgaacttc agcgtgccca tcgaggcgtt gccgaactgg aaatagacgt
     6721 tccggttgtc agtctgggaa taggcgttac ctgcgccgtc gggaaggccg acgatgctgc
     6781 tactgctgtt gatgcccggc gcaaagccga agtgcgcctt gatgtcgtag ccgtcctgtt
     6841 gcgtggtcgc ggcaaagtgg atccagcccg gcagcaggcc gttctgcacg tttgcctgtt
     6901 tgttcgacgt tgtgacaccg gcgccatcgg tggtcttggc gttgctgttg acgtagaaag
     6961 cgttgacggt gccgttgacg ttcagcgtcc
//
LOCUS       DATDAA010000005         4150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_244664,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000005 DATDAA010000000
VERSION     DATDAA010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244664"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VIS73_00210"
     CDS             complement(<1..773)
                     /locus_tag="VIS73_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5761605.1"
                     /translation="MTRRMLICVFTGFASGLPLYLLINLLPAWLRSEGVDLRMIGFFA
                     LVQFPYTWKFFWAPLLDRFSPLRLGRRRGWMLASQVGLFLCIGALGGFDPAGEIGLVM
                     GVATLLAFLSATQDIALDAYRREILAEAELGLGNAVHVNAYRIAGLIPGSLSLILADL
                     LPWSQVFWITALFVLPGFFMTLLVREPTIAAGTPKTLREACVEPFREFVGRVSWRGVF
                     LILGFMVFYKLGDSLATALSTPFYLDMGYSKTDIGLIAK"
     gene            931..1437
                     /locus_tag="VIS73_00215"
     CDS             931..1437
                     /locus_tag="VIS73_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY5761606.1"
                     /translation="MTTPIKYPTPSHRVATLFVLAGLALALGLSACSSAPSRAGAVAG
                     LSDRVAATAADTAAGLTGRPYRYGGASPSGFDCSGLVHYSYARAGQTVARSTDTLWRT
                     SAPISLREIRRGDLLFFDQEGKKSSHLGIWLGDGRFVHAPSSGGKVRVDRLESDYWQR
                     HFVAARRL"
     gene            1553..2098
                     /locus_tag="VIS73_00220"
     CDS             1553..2098
                     /locus_tag="VIS73_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEY5761607.1"
                     /translation="MSHQTLIPRQPVPSLDVALLGGGRYVLGSGAGDRFDLIVFYRGL
                     HCPICAKYLIELNRLEAEFVQRGVKVVAVSSDGEDRAREMSTKTGGGLRFGYELRLAK
                     AREWGLYISTSRGKTSIGIEEPERFSEPGVFLVRPDGTLYYGAVQTMPFARPGFQDLL
                     GAIDFAIAKDYPARGEYTGAV"
     gene            2282..4045
                     /gene="argS"
                     /locus_tag="VIS73_00225"
     CDS             2282..4045
                     /gene="argS"
                     /locus_tag="VIS73_00225"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY5761608.1"
                     /translation="MSHDIRSQLEALVKTALASVVPDSQVPALNFERPKQAAHGDFSC
                     NVAMQIAKSLRRNPRELATLLCAEIPASPLIRKVEVAGGGFINFHLFPAARLAAVSQA
                     LASGATFGRSDAGSGTPVQVEFVSANPTGPLHVGHGRGAAYGASLSSLLAFAGFDVTR
                     EYYVNDAGRQMDILAVSTWLRYLDQLGESVPFPPNGYQGDYVRDMARKFAVMNGERCL
                     RQAGEVLAGTPGLPERERADDEAKQQREKHLDALIAAGKRQLGNDWAFLHDYVLNEQM
                     ADLRDDLAGFGVEFDVWFSEQSLYDTGLVERAVKKLEAAGHIYAQNNAKWFKSTVFGD
                     EKDRCVQRENGLYTYFASDIAYHLNKFERGFRLIIDVWGADHHGYIPRVKGAVTALGL
                     DPVLLNVALVQFAVLYRNGQKASMSTRSGEFVTLRELREEVGNDACRFFYVLRKSDQH
                     LDFDLDLAKSQSNENPVYYIQYAHARVCSMLTQWGGDEMRLVDADLAPLESEREFALA
                     SRLGSFPDVIAAAAADLAPHTVAFYLRDLAADFHTWYNAERVLVDDEALKLARLALAA
                     ATRQVIRNGLALLGVSCPESM"
BASE COUNT          760 a         1443 c         1315 g          632 t
ORIGIN      
        1 tgtttggcga tcaggccgat gtcggtcttc gagtaaccca tgtcgaggta aaacggcgtc
       61 gacagcgccg tcgccagcga atcgccaagc ttgtagaaaa ccatgaagcc gaggatcagg
      121 aagacgccgc gccagctgac ccgcccgacg aactcgcgga agggctcgac gcaggcctca
      181 cgcagcgtct tcggcgtgcc ggctgcaatc gtcggctcgc gcaccagcag cgtcatgaag
      241 aaacccggca gcacgaacag tgcggtgatc cagaaaacct ggctccaggg cagcaggtcg
      301 gcgaggatca gcgacagcga accggggatg agaccggcga tgcgataggc attgacatgc
      361 acggcgttgc cgaggcccag ctcggcctcg gcgagtatct cgcggcggta ggcatcgagc
      421 gcgatgtcct gcgtcgccga aagaaaggcc agcagcgtgg cgacccccat caccagacca
      481 atttcgccgg ccggatcaaa gccgccgagg gcgccgatgc agaggaagag gccgacctgc
      541 gaggcgagca tccaaccccg ccggcgaccg aggcgcagtg gcgaaaagcg atcgagcagc
      601 ggcgcccaga agaacttcca ggtgtacgga aactgcacca gcgcgaagaa gccgatcatg
      661 cgcagatcga caccctcgct gcgcagccag gccggcagca ggttgatcag cagataaagc
      721 ggcagacccg aggcaaaacc cgtaaacaca cagatcagca tccggcgggt catcagcggg
      781 gaaagccagg cgggcatcgg ggagtggagc agcaggccgc gaaatcgggg accgcgattg
      841 tacccgccgc ctgccgggca gcctcggcgc ggacgacgct cagcgtctgg ccggtggtaa
      901 attcggcgcg attcctgccg caaacgacag atgacaaccc caatcaaata cccaacacca
      961 tcgcaccgcg tcgccactct gttcgtgctg gccggactgg ccctagccct gggcctgagc
     1021 gcctgcagca gcgcgccgtc gcgcgccggt gccgtcgccg gcctcagcga tcgcgtcgct
     1081 gcgacggcgg ccgataccgc tgccggattg accgggcgac cgtaccgtta cggtggtgcg
     1141 tcaccgagcg gattcgactg cagcggtctc gttcactaca gctacgcacg cgccggccag
     1201 accgtcgccc gcagcaccga caccctctgg cgcacgagcg caccgatcag cctgcgcgag
     1261 atccgccgcg gcgacctgct gttcttcgac caggagggca agaagtcctc gcaccttggc
     1321 atctggctgg gcgatgggcg cttcgtccac gcgccgtcga gcggcggcaa ggtccgcgtc
     1381 gaccggctcg aaagtgatta ctggcaacga catttcgtcg ccgcacgcag gctgtaggcc
     1441 gacgacagcg cgacggcggc tgccgagacg aaacgcaccg ggaaccgccc tcgccaagcg
     1501 gcctccaacc tgttcgcgcg gctcgacctg cgccccaccc taggagaagc gcgtgagcca
     1561 ccagaccctg attccccgcc agcccgttcc ctcgctcgac gtcgcgctgc tcggcggcgg
     1621 ccgctacgtg ctcggctctg gcgccggcga tcgcttcgac ctgatcgtct tctaccgcgg
     1681 actgcattgc ccgatctgcg ccaaatacct gatcgaactc aatcgcctcg aagcggagtt
     1741 cgtccagcgc ggtgtcaagg tggtcgcggt cagcagcgac ggcgaggacc gcgcccgcga
     1801 aatgtcgacc aagaccggcg gcggcctgcg tttcggttac gagctgaggc tcgccaaggc
     1861 gcgcgaatgg ggcctctaca tcagtacgtc gcgcggcaag acctcgatcg gcatcgagga
     1921 gcccgagcgt ttcagcgaac ccggcgtctt cctggtccgc cccgacggca cgctctacta
     1981 tggcgccgtg cagaccatgc ccttcgcccg cccgggtttt caggatctgc tcggcgcgat
     2041 cgacttcgcc atcgccaagg actacccggc gcgcggcgag tacacgggcg cggtctaggg
     2101 caacgcggac ccgccggacc cggtcgtcac cggagccgca ccccaatccg ctcctgcgtc
     2161 gcctgcgccg cagtccgctt gcagcggccg cggctataat ggcgcgcttc aaggccccga
     2221 cgggcctagc gcagccggcg ccccttgcag ccggcgccaa cggttcactt ctctgcccaa
     2281 catgtctcac gatattcgca gccagctcga agcgctcgtc aagacagcgc tcgccagcgt
     2341 cgtacccgat tcgcaggtgc cggcactcaa tttcgaacgg ccgaaacagg ctgcccacgg
     2401 tgatttctcg tgcaacgtcg caatgcagat tgccaagtcg ctgcgccgca atccgcgcga
     2461 actggcaacg ctgctctgcg ccgagatccc ggcctcgccg ctgatccgca aggtcgaggt
     2521 cgccggcggc ggcttcatca acttccatct gttcccggcc gcccgactcg cagcggtcag
     2581 ccaggcgctc gccagcggcg ccacctttgg ccgcagcgat gccggcagcg gcacgccggt
     2641 gcaggtcgag ttcgtctcgg ccaacccaac cgggccgcta catgtcggcc acggacgcgg
     2701 cgcggcctac ggcgccagcc tgtccagcct gttggctttc gccggcttcg acgtgacccg
     2761 cgagtactac gtcaacgacg ccggccggca gatggacatt cttgcagtct cgacctggct
     2821 gcgctatctc gaccagctcg gcgaatcggt tccgttcccg cccaatggct accagggcga
     2881 ctacgtccgc gacatggcgc gcaagttcgc ggtcatgaat ggcgaacggt gcctgcgcca
     2941 ggccggcgaa gtgctcgccg gcactccggg cctgcccgag cgcgagcgcg ccgacgacga
     3001 ggcgaagcaa cagcgcgaga agcatctcga cgcactgatc gccgccggca aacggcagct
     3061 cggcaacgac tgggccttcc tccacgacta tgtactcaac gagcagatgg ccgacctgcg
     3121 cgacgacctc gcgggtttcg gcgtcgaatt cgacgtctgg ttctcagaac agtcgctcta
     3181 cgacaccggc ctcgtcgagc gcgccgtgaa gaagctcgag gcggccggac acatttacgc
     3241 gcagaacaac gccaagtggt tcaagtccac cgtcttcggc gacgaaaagg accgctgcgt
     3301 gcagcgcgaa aacgggctct acacctactt cgcctccgat atcgcctacc acctcaacaa
     3361 gttcgaacgg ggcttccggc tgatcatcga cgtctggggc gccgaccacc acggttacat
     3421 cccgcgcgtg aagggcgcgg tcaccgccct tggtctggac cccgtgctgc tcaacgtcgc
     3481 gctcgtgcag ttcgccgtgc tttaccgcaa cgggcagaag gcgtcgatga gtacgcgctc
     3541 gggcgagttc gtgacgctaa gggagctgcg cgaggaagtc ggcaacgatg cctgtcgctt
     3601 cttctatgta ctgcgcaagt cggaccagca cctcgatttc gacctcgacc tggcgaaaag
     3661 ccagagcaac gagaacccgg tgtattacat ccagtacgcg cacgcgcggg tctgctcgat
     3721 gcttacgcag tggggcggag acgaaatgcg cttggtcgac gccgacctgg cgccgctcga
     3781 gagcgaacgc gagttcgcgc tggccagccg gctcggcagc tttcccgacg tgatcgccgc
     3841 ggcggcagcc gatctggcac cgcacacggt cgccttctat ctgcgcgatc tcgctgccga
     3901 tttccacacc tggtacaacg ccgagcgcgt gctggtcgac gacgaggcgc tgaagctggc
     3961 gcggctcgcg ctggccgcgg cgacccggca agtcattcgc aatggcctcg cgctgcttgg
     4021 cgtgagttgc ccggaatcga tgtgaggaac gcatgaaacc tgcttcatcc cttgccggca
     4081 aggccaaggc accgcgcagt tcggcgagcc gctcgcgcgg taacacgctg tttggcatct
     4141 tcgtcggcat
//
LOCUS       DATDAA010000006         6218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_209816,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000006 DATDAA010000000
VERSION     DATDAA010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209816"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VIS73_00230"
     CDS             complement(<1..245)
                     /locus_tag="VIS73_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY5761609.1"
                     /translation="MADYKNTLNLIDTPFPMRGDLAKREPQWVADWQANDLYGRIRKA
                     AAGRPRFVLHDGPPYANGDIHIGHAVNKILKDIIVRSR"
     gene            complement(268..1191)
                     /locus_tag="VIS73_00235"
     CDS             complement(268..1191)
                     /locus_tag="VIS73_00235"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY5761610.1"
                     /translation="MQVLRGYHAAASHETALTIGNFDGVHLGHRALLDRLQVRARQKR
                     LLPAVLTFEPHPREFFDPDKAPARLTILREKFELLREYGVGLTMVARFSASFAALSPE
                     AFVRDVLVGGLRTRHLIVGDDFRFGARRAGDFATLRALGASAGFEVEAMGSVICDDER
                     VSSSAVRRALAAGEMTRAAQLLGRPYAIDGRVVHGDKRGRELGFPTANIRIRHNPLPM
                     TGVFAVAVADYCDAPRYGVANLGVRPTVNGVRPLLEVHLFDFARDIYGAHLNVRFLHK
                     LRDERRFPDLDALRQQIAADVIAARRFFETN"
     gene            1409..3010
                     /locus_tag="VIS73_00240"
     CDS             1409..3010
                     /locus_tag="VIS73_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY5761611.1"
                     /translation="MSVKSRLFLLAAVSIGVLVLLIGIAMSSMSQLASQQDDVFQKSQ
                     AQRVAAEASWLGAQAYQIIADAIINRDLAQSRKDWADLLAEANGDLDRLAALADTDQE
                     RKDVTTGRESIKALDSLFQRLMPMLSTQNQIAEELRSLDGQIDREAATIRELLARVAE
                     SMANEAKEADAAFDATRESTIRTITIIAVLTGAALAAFSLWLIGTIIRPLAHAREVAG
                     RIADGDLTGTIRIERDDELGQTLHACDRMQQSLREIARSLQDGANDIASTSSQLAAST
                     NQIAKATEQQSQAASSMAASVEEMSVSISHMSDHASEVRSAASQSDQAAITGGAVIET
                     MVDDSQHAAGSVEAAAAEIRQLEALSERISSIVMVIRDVADQTNLLALNAAIEAARAG
                     EAGRGFAVVADEVRKLAERTGKSTQDIAGVIEEVQRATRSVVTRMEQTVAEVRKGATT
                     SQQAGEAITAIRQDASRVVVAVEEIGNALREQSTASTDIAKRVEQIAQMGEENSAAVE
                     ETAVVASSLENLAGRLQTVAGRFRI"
     gene            complement(3103..4407)
                     /locus_tag="VIS73_00245"
     CDS             complement(3103..4407)
                     /locus_tag="VIS73_00245"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY5761612.1"
                     /translation="MIPGATKPSASPSALRRRLLGSAGITALLLVVSPLAHAARRSKA
                     PTVVAVRIWPADEYTRVTLEHDAPIRFSQFTVRNPDRLVVDLEGVQLNKVLRDIAGGV
                     SASDPYIETLRVGEPRPGVVRLVMQLKAEVAPQVFTLPPFGSYRHRLVLDVYPAEPKD
                     ALLGLLEDLENRSPGDKPLDAVPVDKLPVPDSKPAVARFATVVLDPGHGGEDPGAIGR
                     RGTREKDVTLAVARRLKAKLDADPEMRGVLTRDADFFVPLGKRVQKARRVQADLFVSI
                     HADAWVKPSAHGSSVFVLSERGASSAAARWLANKENESDLIGGVNMDVKDRYLARTLL
                     DLSQTATNSDSRKLAQAVLAELGQVNRLHKPAVEHASFAVLKAPDIPSILVETAFISN
                     PEEERRLRDSAYQDKMAEAILGGIRRFFAANPPLAKSRLARL"
     gene            complement(4404..4868)
                     /gene="tsaE"
                     /locus_tag="VIS73_00250"
     CDS             complement(4404..4868)
                     /gene="tsaE"
                     /locus_tag="VIS73_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY5761613.1"
                     /translation="MSLTLPDAAATEACGAALAAVLVPGLVIWLEGDLGAGKTTLVRA
                     ALRALDFAGPVKSPTYSLVELYNLSRLCLYHFDFYRLRAPEEFEDAGLADYFDGKAAC
                     FIEWPDKAGDFAPAADLRLAFAFSGSGRLLDISAGSARGEHCLATLTVSAPR"
     gene            4875..5987
                     /gene="queG"
                     /locus_tag="VIS73_00255"
     CDS             4875..5987
                     /gene="queG"
                     /locus_tag="VIS73_00255"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEY5761614.1"
                     /translation="MIGSMHDAISPQVTRTGARLDCTRLWPEIAQWATELGFSAAGVA
                     ALDMDAPSRHLERWLALGRQGEMDYMARHAALRAQPSQLLPSAQCAISVRHDYWPAAA
                     DPWATLADGKRAYVSRYALGRDYHKTLRRKLQTLATRLGERIGPYGYRVCVDSAPVFE
                     SELAARSGVGWRGKHTLTLTRTGSWHFLGEILTDLPLPPTAAIEDHCGSCTRCLDLCP
                     TGAIVAPYEVDARRCISYLTIEHRDSIPLELRAAMGNRIYGCDDCQLVCPWNRFARRS
                     GDPDFAVRNHLDAAALVELFAWSEHDFNERLAGSPIRRIGWERWLRNIAVALGNALRA
                     KPSVELHDALGSRIDHPSAVVREHARWAMAADDDHH"
     gene            complement(6009..>6218)
                     /locus_tag="VIS73_00260"
     CDS             complement(6009..>6218)
                     /locus_tag="VIS73_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761615.1"
                     /translation="DLLVTYQDVWRWDLKTYLFSLRIDLFDAPTGSLLTTGTWKNSAF
                     HGFQRGKDETRELLSDMLPRIRRGQ"
BASE COUNT         1169 a         2048 c         2036 g          965 t
ORIGIN      
        1 cgagagcgga cgatgatgtc cttgaggatc ttgttcaccg catggccaat gtggatgtcg
       61 ccgttggcgt agggcgggcc gtcgtgaagg acgaaacgcg gccggccggc ggccgccttg
      121 cggatgcgcc cgtagagatc gtttgcctgc cagtcggcga cccactgtgg ctcgcgcttg
      181 gcgagatcgc cgcgcatcgg aaagggcgtg tcgatcagat tgagggtgtt cttgtaatcg
      241 gccatggtgt cgggctcgta ataagcgtca gttcgtttcg aaaaagcgac gcgcggcgat
      301 gacatcggcg gcgatctgct ggcgcaacgc gtcgagatcg ggaaagcgcc gctcatcgcg
      361 cagcttgtgc aggaagcgta cgttcaggtg cgcgccgtag atgtcgcggg cgaagtcaaa
      421 caggtgtacc tcgagcagcg gacgcacgcc gttgacggtg gggcgaacgc cgaggttggc
      481 aacgccatag cgtggcgcat cgcagtaatc ggcgaccgca accgcaaaaa ccccggtcat
      541 tggcaggggg ttgtggcgta tgcggatgtt cgcggtcggg aaacccagct cgcggccccg
      601 cttgtcgccg tggacgactc ggccgtcgat cgcgtagggc cgtccgagca gctgcgcagc
      661 gcgggtcatc tccccggcgg cgagggcacg acgaaccgca gagctcgaga cgcgttcgtc
      721 gtcgcagatg acactgccca tcgcctcgac ctcgaaacca gccgacgcgc cgagcgccct
      781 cagcgtcgcg aagtcgccgg cgcgcctggc gccgaagcgg aaatcatcac cgacgatcag
      841 gtggcgcgta cgcaagccgc cgaccagcac atcgcggacg aaggcttcgg gtgagagcgc
      901 cgcaaagctc gccgagaagc gcgcgaccat cgtcagcccg acgccgtact cacgcagcag
      961 ctcgaacttc tcgcgcagga tcgtcagccg cgccggcgcc ttgtccggat cgaaaaactc
     1021 tcgcgggtgc ggctcgaagg tcagcaccgc cggcagcagc cgtttctgcc gggcgcgtac
     1081 ctgcagacgg tcgagcagcg cgcggtgccc gaggtggacc ccgtcgaagt tgccgatcgt
     1141 gagcgccgtc tcatgcgacg cagcagcgtg atagccgcgg agaacttgca tttggcccga
     1201 tgttggctaa ccgaacggta agccgctgaa aagcggccga ttatagcggt tccacgagca
     1261 gaaggccggc ggcgcccacc gtaccgggcc acgacgctga cgcctactca aatgaggtat
     1321 gactttagtc tgattgtgcg attgctgcgc cgcatctaag atgccccgcg ctggtctgac
     1381 caaaaaatat cattgcacgg agccttccat gagcgtcaag tcgcgccttt ttcttctcgc
     1441 cgccgtttca attggcgtct tggtcctgct gatcggcatc gccatgtcgt cgatgtcgca
     1501 gctggccagc cagcaagacg acgtattcca gaaatcgcag gcccagcgcg ttgccgccga
     1561 ggcctcttgg ctcggtgcgc aggcctacca gatcattgcc gacgcaatca tcaatcgcga
     1621 tctcgcgcaa tcgcgcaagg actgggccga cctgctggcc gaggccaatg gcgatctcga
     1681 ccggctggcg gcgctggccg acaccgacca ggaacgcaag gacgtcacta ccggacgcga
     1741 atcgatcaag gcgctcgaca gcctattcca gcgcctgatg ccgatgctct caacgcagaa
     1801 tcagattgcc gaggaactcc gctccctcga cgggcaaatc gatcgcgagg ccgcgacgat
     1861 ccgcgaactg ctggcccggg tcgccgaatc gatggcgaac gaagcaaagg aggccgatgc
     1921 cgcgttcgat gccacccgcg agagcacaat ccgcacaatc accatcatcg ccgtgctgac
     1981 cggcgccgca ctcgctgcgt tttcgctatg gctgatcggc accatcatcc ggccgctcgc
     2041 ccacgcgcga gaagttgccg ggcgaatcgc cgacggcgac ctgaccggca cgatccgaat
     2101 cgaacgcgat gacgagctgg gacagaccct gcacgcctgc gaccgcatgc agcagagcct
     2161 gcgcgaaatc gcccgctcgc tgcaggatgg cgccaacgac atcgccagca ccagcagcca
     2221 gcttgccgct tcgaccaacc agatcgccaa ggcgaccgag cagcagtcgc aggccgcctc
     2281 gtcgatggcg gcctcggtcg aggaaatgtc ggtcagcatc agccacatgt cggaccacgc
     2341 cagcgaagtg cgcagcgccg cctcgcaatc cgatcaggcg gcgatcaccg gcggcgccgt
     2401 gatcgagacg atggtcgacg actctcagca cgccgcgggg tcggtggaag ccgccgccgc
     2461 ggagattcgc cagctcgaag cgctgtcgga gcgaatctcg tcgatcgtca tggtgattcg
     2521 cgatgtcgcc gatcagacca acctgctcgc cctcaacgcc gcgatcgagg cggcgcgcgc
     2581 cggcgaggcc ggccgcggct tcgcggtcgt cgccgacgaa gtacgcaagc tcgccgagcg
     2641 caccggcaag tcgacccagg acatcgccgg ggtcatcgag gaagtgcagc gcgcgacgcg
     2701 ctcagtcgtc acgcgcatgg agcaaaccgt tgccgaagtg cgcaaggggg cgacgacctc
     2761 gcagcaggcc ggcgaggcaa tcacggcgat ccgccaggat gcctcgcggg tcgtggtcgc
     2821 ggtcgaggaa atcggcaatg cgctgcgcga gcagtcgacg gccagcaccg acatcgccaa
     2881 acgggtcgaa cagatcgcgc agatgggcga agagaacagc gcggccgtcg aggaaacggc
     2941 ggttgtcgcg agcagcctgg agaacctcgc cggtcgactg cagaccgttg ccggtcgttt
     3001 ccgaatctga gcaaccatag ggccgggctt cggccagccc gacccccttt ggcagtaaag
     3061 gaaactcggg gaaccccttc gttttcttgg ggtgtatccg ccctacaggc gtgcgagccg
     3121 ggacttggcg agcggcgggt tggcggcgaa aaagcggcgg atgccgccga gaatcgcctc
     3181 ggccatcttg tcctgatagg ccgaatcgcg cagccggcgt tcttcttctg gattgctgat
     3241 gaaggcggtc tcaacgagga tagagggaat gtccggcgcc ttgagcacgg cgaaactggc
     3301 gtgctcgacg gccggcttgt gcaggcgatt gacctgcccg agttcggcaa gtacggcctg
     3361 cgccagcttg cggctgtcgc tgttggtcgc agtctgcgag aggtcgagca gcgtgcgcgc
     3421 cagataacga tccttgacgt ccatgttcac gccgccgatc aggtcggact cgttctcctt
     3481 gttcgccagc cagcgggccg ccgcgctcga tgcgccacgc tcggaaagga cgaagaccga
     3541 agagccgtga gcgctcggct tcacccaggc atcggcgtga atcgacacga acagatcggc
     3601 ctgcacgcgg cgcgccttct gcacccgttt accaagcggc acgaagaaat ccgcgtcgcg
     3661 cgtcagcaca ccgcgcattt ccggatcggc atcgagcttg gccttgagcc gacgggccac
     3721 ggccagtgtc acgtctttct cgcgcgtgcc gcgccggccg atcgcaccgg ggtcttcacc
     3781 gccgtgccca ggatcgagaa cgacggtggc gaaacgggcg accgccggct ttgaatccgg
     3841 caccggcagc ttgtcgactg gcaccgcgtc gagcggcttg tcgcccggcg agcggttctc
     3901 gaggtcttcg agcaggccga gcagcgcgtc cttgggctcg gccggataga cgtcgagaac
     3961 caggcgatgc cggtacgagc cgaagggcgg cagcgtaaac acctgcggcg caacttcagc
     4021 cttcaactgc atgaccagcc gcacgacgcc cggacgcggc tcgccgacgc gcagcgtctc
     4081 gatgtagggg tcgctggcgc tgacaccgcc ggcgatgtcg cgcagcacct tgttgagttg
     4141 aaccccctcg aggtcgacga cgagccgatc gggattgcgc accgtgaact gcgagaagcg
     4201 gattggcgca tcgtgctcca gcgtgacccg cgtgtactcg tcggcgggcc agatgcgaac
     4261 ggcaaccacg gttggcgcct tgctccggcg ggcagcgtgc gccagcggcg aaaccaccag
     4321 cagcagcgcg gtaatcccgg ccgatccgag caggcgacgg cgcagggcgc tgggcgaagc
     4381 ggagggcttg gtggcgcccg gaatcagcgg ggcgcggaaa ccgtcagcgt ggcgagacag
     4441 tgctcacccc gcgcgctgcc tgcggagatg tcgagaagcc tgccgctacc gctgaatgcg
     4501 aacgccagac gcagatcggc cgccggcgcg aaatcgccag ccttgtcagg ccactcgatg
     4561 aaacaagcgg ccttgccgtc gaagtaatcc gcgaggcctg catcttcaaa ttcttccggc
     4621 gctcttagcc tatagaaatc aaagtgatat aagcacaatc tcgaaagatt gtaaagttca
     4681 accaggctgt aggtcgggct ttttaccggc ccggcgaaat ccagcgcgcg cagcgcggca
     4741 cggaccagcg tagtcttgcc tgcgcctaga tcaccttcga gccagatcac cagcccaggc
     4801 accagcacgg ccgccagcgc cgcgccgcag gcttcggtgg cagcggcatc gggcagggtc
     4861 agggacaagg acttatgatc ggcagcatgc acgacgcgat ttctccgcaa gtgactcgca
     4921 ccggcgcccg cctggactgc acccggctct ggccggagat cgcgcaatgg gcgaccgaac
     4981 tggggttctc ggcggcaggc gtcgccgcgc tcgacatgga cgcgccctca cggcatctgg
     5041 aacgctggct ggcgctgggc cggcagggcg agatggatta tatggcacgg cacgcagcac
     5101 tccgcgcgca gcccagccaa ctgctgccga gcgcccaatg cgcgatcagc gtccgccacg
     5161 actactggcc tgctgccgcg gacccatggg cgacgctggc cgacggaaaa cgcgcctacg
     5221 tgtcgcgcta tgcgctgggt cgcgactacc acaagacact gcggcgcaag ctgcagacac
     5281 tggcaacgcg cctcggcgaa aggatcggcc cgtacggcta ccgcgtctgt gtcgattcgg
     5341 caccggtctt cgaaagcgaa ctagccgctc gctcgggcgt cggctggcgc ggcaagcaca
     5401 ccctgacgct gacccgcaca ggctcctggc atttcctcgg cgagatcctc accgatctgc
     5461 cgctaccacc gacagcagcg atcgaagatc actgcggcag ctgcacgcgc tgcctcgacc
     5521 tctgcccgac gggagcgatc gtcgcgccct acgaggtcga cgcgcgccgc tgcatttcct
     5581 acctgacgat cgagcacagg gacagcattc cgcttgaact acgcgcagcg atgggcaacc
     5641 gcatctacgg ctgcgacgac tgccagctgg tctgcccgtg gaatcgcttt gcgcggcgga
     5701 gcggggaccc cgacttcgcc gtccgcaacc accttgacgc ggcagcgctg gtcgagttgt
     5761 tcgcctggag cgaacacgat ttcaacgagc gcctcgccgg ctcgccgatc cgccgcatcg
     5821 gttgggaacg ctggctgcgc aacatcgcag tcgcgctcgg caacgcgtta cgggcgaaac
     5881 cgtccgttga gctgcatgac gcgttaggga gccgcataga ccacccttcg gcagtcgtac
     5941 gtgagcatgc ccgctgggcc atggcggccg acgacgacca ccattagcgc tatccttgca
     6001 ggggcagact actgaccgcg gcgaatcctc ggcagcatgt cactcagcaa ctcgcgcgtt
     6061 tcatccttgc cgcgctgaaa gccatgaaag gcggagttct tccaagtgcc agtcgtaagc
     6121 agcgaaccgg tcggcgcatc gaacaagtcg atgcgcaatg agaacaggta ggtcttcagg
     6181 tcccagcgcc agacgtcctg ataggtcacc agcagatc
//
LOCUS       DATDAA010000007         7621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_314601,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000007 DATDAA010000000
VERSION     DATDAA010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314601"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VIS73_00265"
     CDS             <1..181
                     /locus_tag="VIS73_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylglutamate synthase"
                     /protein_id="HEY5761616.1"
                     /translation="RSAQRARSSGVRELFVLTTQTTHWFIERGFVAVGVERLPDEKQL
                     LYNYQRRSKVLILPL"
     gene            211..483
                     /locus_tag="VIS73_00270"
     CDS             211..483
                     /locus_tag="VIS73_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEY5761617.1"
                     /translation="MTRMVNCIKLGREAEGLERPPYPGALGQRIFEQVSKDAWNQWIR
                     MQTMIINENRLNLVDAAHRKYLREQVEKHFFGEGADQVQGYVPPKS"
     gene            complement(511..1068)
                     /locus_tag="VIS73_00275"
     CDS             complement(511..1068)
                     /locus_tag="VIS73_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761618.1"
                     /translation="MAVFSKPFSIATLSAALSCTGCAGILFDSSRHSEIDRPVPRMAD
                     KGFFRERWACMPPDESSARATREDFLRHWGEPKSRQLDGRKETWRYGEDGRWCGIWLI
                     AVVPIPLLVPICNTYDMVEFEDGIALRSASRRFHVTGIFATIAPYPGAAILREGAVTE
                     DRPNRPVGSSPEPCAWPLPEGDAAR"
     gene            complement(1155..1772)
                     /locus_tag="VIS73_00280"
     CDS             complement(1155..1772)
                     /locus_tag="VIS73_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761619.1"
                     /translation="MSSFGIPAATAMLAAAASCAGCVGVSLDMPRTAELSHPAPLVAA
                     GAMPTAYKSRWACQSPEGVFPPLGPGDFLSAWGEPASRRTTAGGEIWTYAERGRWCGA
                     WIFAIVPLPLLLPICDTYDEVTFEDGRAVRARSRRFHLTGIGVGFAPQAFFFPVPYRV
                     RAGTTAEANPDMLELFPMVPEARSICTWSPGDASRPVKTSAESSG"
     gene            complement(1865..2524)
                     /gene="rpiA"
                     /locus_tag="VIS73_00285"
     CDS             complement(1865..2524)
                     /gene="rpiA"
                     /locus_tag="VIS73_00285"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEY5761620.1"
                     /translation="MNQDELKKAVANAALNYVEAGKIIGIGTGSTANHFIDALAARRA
                     EIPGAVASSEATRQRLEGHGIRVLDLNTVSEMYLYVDGADEINESMHMIKGGGGALTR
                     EKIVAEVAERFVCIADESKLVATLGKFPLPVEVIPMAQALVERRLRALGGEPKLRAGF
                     TTDNGNLILDLHGLSITDPITLETALNQIPGVVTNGLFARRPADVLLLGGLQGVRRLS
                     A"
     gene            complement(2541..3185)
                     /locus_tag="VIS73_00290"
     CDS             complement(2541..3185)
                     /locus_tag="VIS73_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY5761621.1"
                     /translation="MKPVSALAAAALALCAFDTASALAAPDEPPAMLLVASPKIDDPR
                     FRESVVLVLRHGRGGPLGVIVNKGSDVTLATVFKKPEEAKLAAKPLHFGGPIEPQRLI
                     ALYRDPQPQPRDSLQIADGLWMSQSQARIERVLERPPESFKIMAGFSGWARGQLEHEI
                     ARGDWYLLPLDQSVLFGADDATLWSTLLQRASQRSAALPSRPPTRTAEPHPLPA"
     gene            complement(3182..4897)
                     /locus_tag="VIS73_00295"
     CDS             complement(3182..4897)
                     /locus_tag="VIS73_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761622.1"
                     /translation="MLNWITGKFRQDATDNPDHPLGTEAAIQEFLADLAPSRHENNLL
                     EVVAWLSEPERISADLSTDGYLRAIMRLDEFAQGQALALWTHVLAERRIDHLVEQKLR
                     TLDSFYNARHQANLAALKVLRDKPEVGGPNAATLRGLIAQRAMAGLAGKLRVMRVRYR
                     SPDAHWWEAVTVILRISQAMGVINLRQRSYPDDASPSSCWVELLIVLFFEVAPVGNCT
                     PQQMELLYRVLRWLEPHFMVQDTYSPQAQWMVRLDSASAPVKSIGAVAPDPALVFFGP
                     GLAYSRLIGFRTTLATAKETPDWLASTYCAKDAALATVDSLIMHWSERPPQRRTKRTQ
                     NDSHLRVASGFSNIRRVIAFSEFARSGRKIGYKSHLEMLKFERRGFADSVQVSKDDEE
                     KWQNASPLQTLEILETAGDKQMMDDWISQDVSETGIGATAPFLRPWMSIGVYAGYRQH
                     GEVDWRIGIVRRIHRTEHGHPSVGIETFSEVPSCAQVRRIVLVKPDANPWHEATREGG
                     TFEDAIVLSMMKSLILLPAGMFMGGAHMVLMLSGQRFPIKLLELVHRNPDCDCIRFEM
                     CDQDE"
     gene            complement(4989..6779)
                     /gene="lpdA"
                     /locus_tag="VIS73_00300"
     CDS             complement(4989..6779)
                     /gene="lpdA"
                     /locus_tag="VIS73_00300"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY5761623.1"
                     /translation="MSNLVEIKVPDIGDFSDVPVIDVFIKPGDTVAVEDALVTLESDK
                     ATMDVPATAGGVVKEVRVKLGDKVSEGTVVAVVESAAASAGTPAAKAAPAPAAAAAPA
                     ASPTASAPAAASHGGSADIECDMLVLGAGPGGYSAAFRAADLGLKTVIVERYATLGGV
                     CLNVGCIPSKALLHVAAVMDEAEHMAAAGISFAKPKVDLKALRAHKDKVVGKLTGGLA
                     GMAKGRKVEIVRGYGHFLDPNHLEVEETKGDGQDKTGAKKVVRFKNCIIAAGSAAVHL
                     PFIPRDERVVDSTGALELRQVPKKMLVIGGGIIGLEMATVYSTLGTRVDVVEMLDGLM
                     QGPDRDAVKVWEKQNAQRFDNVFLKTKTTAVEAKKDGLHVSFEGEKAPKGPQRYDMIL
                     QSAGRRPNGHKIGADKAGVIVDERGFIPVDPQMRTNVPHIFAIGDIVGNPMLAHKAVH
                     EAHVAAEVAAGQKSAFDATVIPGVAYTHPEVAWVGYTEDQAKQEGRKVETAKFPWAAS
                     GRAIANGADYGFTKLIFDAETHRVIGGAIVGPSAGDMIGEVCLAIEMGADAVDIGKTI
                     HPHPTLGETVGMAAEVAHGSCTDLPPVKKK"
     gene            complement(6797..>7621)
                     /locus_tag="VIS73_00305"
     CDS             complement(6797..>7621)
                     /locus_tag="VIS73_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEY5761624.1"
                     /translation="EDVTAFVKGVMSGGGAAAAAGGSGAITGGGTLNLLPWPKVDFAK
                     FGEIEVKELSRIKKLSAANLARNWVMIPAVTYHEDADITDLEAFRVQVNKENEKSGKK
                     LTMLAFLMKASVRAMQKFPEFNASLDGDNLVLKKYFNIGFAADTPNGLVVPVVKHCDK
                     KSVFEIAAETGELAKQARDGKLKPADMQGACFTISSLGGIGGTYFAPIVNAPEVAILG
                     VNKSAIKPVWDGKTFQPKLILPLSLTADHRVIDGALATRFNVFLAELLADMRRVLL"
BASE COUNT         1256 a         2378 c         2556 g         1431 t
ORIGIN      
        1 aagaagtgct cagcgggcgc gcagcagcgg agttcgcgag ctgttcgtgc tgacgacgca
       61 gaccacgcac tggttcatcg agcgcggctt cgtggctgtc ggcgtcgaac gcctgcccga
      121 cgagaaacaa ttgctctaca actaccagcg ccggtccaag gtgctgatcc tgccgctcta
      181 ggcggcagtc aacgataagg agaggtatgc atgacgcgca tggttaactg catcaagctc
      241 ggtcgcgaag ccgaggggct ggaacgacca ccgtatccgg gcgcactcgg ccagcggatc
      301 ttcgagcagg tgtcgaagga tgcctggaac cagtggattc gcatgcagac gatgatcatc
      361 aacgagaacc gcctcaatct cgtcgatgcc gcgcaccgca agtacctgcg cgaacaggtc
      421 gagaagcact tctttggcga gggtgccgat caggtgcagg gttacgtgcc tccgaagtcc
      481 tgatcgacag cggtggtggc cgcagcgcgt ctagcgcgcg gcatcgcctt caggtaacgg
      541 ccatgcacac ggttcggggc tggagccgac cgggcggttc gggcggtctt cggtcaccgc
      601 gccttccctg aggattgcgg cacctgggta gggggcgatc gtggcaaaga taccggtcac
      661 gtggaatcgg cggctggccg agcggagcgc gattccgtcc tcgaactcga ccatatcgta
      721 cgtattgcag atcggcacca gcagcgggat cggcacgact gcgatcagcc agatgccgca
      781 ccagcgtccg tcctcaccgt aacgccaggt ttcctttcgg ccgtccaact gacggctctt
      841 cggctcgccc cagtgccgca ggaagtcctc gcgggtcgcg cgggccgacg actcgtcggg
      901 cggcatgcac gcccatcgtt ccctgaaaaa gcccttgtcc gccatccgcg ggaccggccg
      961 atcgatttcc gaatggcgcg agctgtcgaa gaggattccg gcgcaacccg tgcaggatag
     1021 tgctgccgaa agcgttgcga ttgagaacgg tttgctgaag acggccatag aggaactccc
     1081 gtcagttgtt cgtgtgctac ggccgggcca ggccgttacg atcgaacgtg ccgggtcgtg
     1141 agacgcgggc tgtcctaacc cgacgattcc gcgctggtct tcaccggacg cgaagcgtct
     1201 cccggcgacc aggtgcagat gctgcgcgct tcgggaacca tcgggaacag ttcgagcatg
     1261 tcgggattcg cctcggcggt cgttccggcg cgcacgcgat aggggaccgg gaaaaaaaag
     1321 gcctggggcg cgaagcccac gccgatcccg gtcaggtgaa agcgccggct ccgcgcacgc
     1381 accgcgcggc catcctcgaa agtcacctcg tcgtaggtgt cgcagatcgg cagcaacaac
     1441 ggcagcggaa cgattgcgaa gatccatgcg ccacaccacc ggccacgctc ggcgtaggtc
     1501 cagatttcgc ccccggcggt cgtccggcgg ctggcgggtt cgccccaggc ggagagaaag
     1561 tcgcccggtc cgagcggcgg gaagacacct tcgggcgact ggcaggccca gcgtgacttg
     1621 taggcggttg gcatcgcccc cgccgccacc agcggtgccg gatggctgag ttcggcggtg
     1681 cgcggcatat cgagagagac accgacgcag cccgcacagc ttgccgccgc cgccaacatc
     1741 gccgttgccg ccggaatgcc gaagctgctc atccgatgtg cctcgtcctg ccggctcttg
     1801 ccggcgaagg gccatgctag tcgcttgtgg tccggtttgc cagtgcgccg gcaacgcggt
     1861 gcggctaggc gctgagccgg cgcacgccct ggaggccgcc aagcagcagc acatcggccg
     1921 ggcggcgggc gaacaggcca ttggtgacga cgcccggaat ctggttcagc gcggtttcca
     1981 gcgtgattgg atcggtgatt gaaagaccgt gcaggtcaag gatcaggttg ccgttgtccg
     2041 tggtgaagcc ggcgcgcagt ttcggctcgc cgcccagtgc gcgcagccgg cgttcgacca
     2101 gcgcctgcgc catcggaatt acctcgacgg gcagcggaaa cttccccagc gtcgcgacca
     2161 gtttcgattc gtcggcgatg cagacgaacc gctccgcgac ctcggcgacg atcttttcgc
     2221 gcgtcagtgc gccgccaccg cccttgatca tgtgcatgga ttcattgatc tcgtcggcgc
     2281 cgtcgacgta gagatacatc tcgctcaccg tgttgagatc gagcacgcgg atgccgtgtc
     2341 cttcgagtcg ctggcgcgtg gcttccgagc tggcgacggc accggggatc tctgcacggc
     2401 gggccgcgag tgcgtcgatg aagtgattgg ccgtcgagcc ggtgccgatg ccgataatct
     2461 tgccggcttc gacgtagttg agcgcggcgt tggcgacggc ttttttcagt tcgtcctgat
     2521 tcatgggctc ctcgggggta ctaggcgggc agcgggtgtg gttccgcggt gcgtgtcggt
     2581 gggcggctgg gcagcgctgc gctgcgctgg ctcgcgcgtt gcagcagcgt cgaccagagt
     2641 gtggcgtcgt cggcgccgaa cagcacggac tggtcgagcg gaaggaggta ccagtcaccg
     2701 cgcgcgatct cgtgttctag ctgcccgcgc gcccagccgg agaagccggc catgatcttg
     2761 aagctttccg gcgggcgctc gagcacgcgt tcgatcctcg cctgcgactg gctcatccaa
     2821 aggccatcgg cgatctgcag cgaatcgcgc ggttgtggtt gcggatcgcg gtagagcgcg
     2881 atcaggcgct gcggttcgat cggcccgccg aagtgaagcg gcttcgcggc gagcttcgct
     2941 tcctcgggtt tcttaaacac agttgccagc gtgacgtcgg agcccttgtt gacgatcacg
     3001 ccgagcgggc ctccgcgacc gtggcggagc accagtacga cgctttcgcg gaaacgcggg
     3061 tcgtcgatct tgggactggc aaccagcagc atcgctggcg gttcatcggg tgctgccagc
     3121 gccgacgcgg tgtcgaaggc gcagagcgcg agcgctgcgg cggcgagcgc gctgacgggt
     3181 ttcactcgtc ctggtcgcac atctcgaagc ggatgcagtc gcagtccgga ttgcgatgca
     3241 ccagttcgag cagcttgatc gggaaacgct ggccggacag catgagcacc atgtgcgcgc
     3301 cgcccatgaa catgccggca ggcaacaaga tcaggctctt catcatcgac aggacgattg
     3361 cgtcctcgaa ggtgccgcct tcgcgcgttg cttcgtgcca tgggttggcg tcgggcttga
     3421 ccagcacgat gcgccgcacc tgcgcgcaac tgggtacttc ggaaaaggtt tcgatgccga
     3481 ccgagggatg gccgtgttcg gtgcggtgga tgcggcggac gatgccgatc cgccagtcga
     3541 cctcgccgtg ctgacggtag ccggcgtaga cgccgatgct catccacgga cgcagaaagg
     3601 gcgccgtggc gccgattccg gtctcgctga cgtcctgcga gatccagtcg tccatcatct
     3661 gcttgtcgcc ggcggtttcg aggatttcca gcgtttgcaa tggcgaagcg ttctgccact
     3721 tctcctcgtc gtccttgctg acctgcaccg aatcggcaaa gccgcgacgc tcgaacttga
     3781 gcatctcaag gtggctctta tagccgatct tgcggcccga gcgagcgaac tcggagaagg
     3841 caatgacgcg acgaatgttg gagaaaccac tggcgacgcg caggtgcgaa tcgttctgtg
     3901 tgcgcttggt ccgcctctgc ggcgggcgct ctgaccagtg catgatcagg ctatcgacgg
     3961 ttgccagtgc ggcatctttg gcgcaatagg tactcgccag ccagtcgggg gtctccttgg
     4021 ccgtcgccag cgtcgtgcgg aagccgatca gccggctgta ggcgaggcct gggccgaaga
     4081 acaccagtgc cggatcgggc gcgacggcac cgatgctctt gaccggcgcc gacgcgctgt
     4141 cgaggcggac catccattgc gcctgcggcg aataggtgtc ctggaccatg aagtgcggct
     4201 ccagccagcg cagcacgcga taaagcagtt ccatctgctg tggcgtgcag ttgccgaccg
     4261 gggcgacctc gaagaacagc acgatcagca gttcgaccca gcacgatgac ggcgaggcgt
     4321 catcggggta gctgcgctga cgcaggttga tgacacccat cgcctgtgaa atcctgagga
     4381 ttaccgtgac ggcttcccac cagtgggcgt cgggcgaacg gtagcgcacg cgcatcacgc
     4441 gcagcttgcc ggcgaggccg gccatggccc gctgggcgat gaggccgcgc agggtggcgg
     4501 cgttcgggcc gccgacctcg ggcttgtcgc gcaggacctt cagcgccgcc aaattcgcct
     4561 ggtggcgggc gttgtagaag ctgtccaggg tacgcagctt ctgctcgacc agatgatcga
     4621 tgcggcgttc ggcgaggacg tgcgtccaca gcgccagcgc ctgtccctga gcgaattcgt
     4681 cgaggcgcat gattgcgcgc aggtagccgt cggtgctgag gtcggccgaa atccgctcgg
     4741 gctcggagag ccaggcgacc acttcgagca ggttgttttc gtggcggctc ggcgcgaggt
     4801 cagcgaggaa ttcctggatc gccgcctcgg tgccaagtgg gtgatcgggg ttgtcggtag
     4861 catcctgtct gaatttgccg gtaatccagt taagcatgcc gaatccctca cgatcagtgt
     4921 ttataggtgg tcatgagacc ttaatattgc cgagaactgt agcataatca ttgggttatt
     4981 tctcgatctt atttcttttt caccggtggc aggtcggtgc aggaaccgtg tgccacctcg
     5041 gcggccatgc caaccgtttc gccaagtgtc ggatgcggat ggatggtctt gccgatatca
     5101 accgcgtcag cacccatttc gatcgccagg cagacttcgc cgatcatgtc accggcggag
     5161 gggccgacaa tggcgccgcc gatgacacga tgcgtttcgg catcgaaaat cagcttggtg
     5221 aagccgtagt cggcaccatt ggcgatggcg cggcccgagg ccgcccaagg gaatttggcg
     5281 gtctcgacct tgcggccttc ctgcttcgcc tgatcctcgg tgtagccgac ccaggcgact
     5341 tccgggtgcg tgtaggcgac gcctgggatc accgtcgcat cgaaggccga cttctgcccg
     5401 gccgcgactt cggcggcaac atgcgcttcg tgcacggcct tgtgggcgag catcgggttg
     5461 ccgacgatgt cgccgatcgc aaagatgtgc ggcacgttgg tgcgcatctg cgggtcgacc
     5521 ggaatgaagc cgcgttcgtc gacgatcacc ccggccttgt cggcgccgat cttgtgcccg
     5581 ttggggcggc ggccggccga ctgcaggatc atgtcgtagc gctgcgggcc tttcggggcc
     5641 ttctcgcctt cgaagctgac gtggaggcca tccttcttgg cttcgaccgc cgtggtcttc
     5701 gtcttcagga aaacgttgtc gaagcgctgg gcgttctgct tttcccaaac cttgaccgcg
     5761 tcgcggtcgg gaccctgcat caggccgtcc agcatctcga cgacgtcgac gcgggtgccg
     5821 agcgtcgagt acacggtggc catttccagg ccgatgatgc cgccgccgat gaccagcatc
     5881 ttctttggca cctggcgcag ttcgagcgca ccagtcgagt cgacgacgcg ctcgtcgcgc
     5941 gggatgaagg gcaggtgcac ggcggccgaa ccggcggcaa tgatgcagtt cttgaagcgc
     6001 acgaccttct tggcgccggt cttgtcctgg ccgtcgccct ttgtttcctc gacctccaga
     6061 tggttcgggt cgaggaagtg gccgtagccg cgaacgatct cgacctttcg ccccttggcc
     6121 atgccggcga ggccaccggt gagcttgccg acgaccttgt ccttgtgcgc acgcagcgcc
     6181 ttgaggtcaa ccttgggctt ggcgaagctg atgccggcag cggccatgtg ttcggcctcg
     6241 tccatcaccg cggcgacatg cagcagcgcc ttcgacggaa tgcacccgac gttgaggcag
     6301 accccgccga gcgtcgcata gcgctcgacg atcaccgtct tcagcccgag gtcggcggcg
     6361 cggaaggcag ccgaatagcc gccgggaccg gcaccgagta cgagcatgtc gcattcgatg
     6421 tcggcactgc caccgtggct ggccgccgcg ggagcgcttg ccgtgggcga agcggccggt
     6481 gccgcggcgg ccgctggggc aggcgcggcc ttggcagccg gtgtccccgc gcttgcagcc
     6541 gccgattcga ccacggcgac gacagtaccc tcgctgacct tgtcgccgag cttaacccgt
     6601 acttccttga ccacgccgcc ggcggtggcc ggtacgtcca tcgtggcctt gtcggactcg
     6661 agcgtgacca gcgcgtcttc caccgcaacg gtgtcgccgg gcttgatgaa gacgtcgatg
     6721 accggaacgt cgctgaagtc cccgatgtcc gggaccttga tctctaccag attgctcatc
     6781 gttctctcct tcgggcttag agcagaacgc gccgcatgtc ggcgagcagt tcggcaagga
     6841 agacgttgaa gcgtgtcgcc agcgcgccat ctatgacgcg gtggtcggcg gtgagcgaca
     6901 gcggcaggat cagtttcggc tggaaggtct tgccgtccca gaccggcttg attgcactct
     6961 tgttgacacc gaggatcgcg acttccggcg cattgacgat gggcgcgaag taggtaccgc
     7021 cgatgccgcc gagcgaactg atcgtgaagc aggcgccctg catgtccgcc ggcttaagct
     7081 tgccgtcgcg cgcctgtttg gccagttcgc cggtttccgc agcgatctcg aacacgctct
     7141 ttttgtcgca gtgtttcact accggaacga ccaggccgtt cggtgtgtcg gcggcgaagc
     7201 caatgttgaa gtacttcttg agaaccagat tgtcgccgtc gagcgaggca ttgaactcgg
     7261 ggaatttctg catcgcgcgg acgctggcct tcatcaggaa ggcgagcatg gtgagcttct
     7321 tgccgctctt ttcgttctcc ttgttcacct gcacgcggaa tgcttcgaga tcggtgatgt
     7381 cggcgtcctc gtggtaggtg acggccggga tcatcaccca gttgcgcgcg aggttggcgg
     7441 ccgagagctt cttgatccgg gacagctcct tcacctcgat ttcgccgaat ttcgcgaagt
     7501 cgacctttgg ccagggcagc aggttgagcg tgccgccacc cgtgatcgcg ccagagccgc
     7561 cggctgcagc ggcggcccca ccgccgctca tcacgccctt gacgaaggcg gtgacgtctt
     7621 c
//
LOCUS       DATDAA010000008         4770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_209940,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000008 DATDAA010000000
VERSION     DATDAA010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209940"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VIS73_00310"
     CDS             <1..352
                     /locus_tag="VIS73_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761625.1"
                     /translation="CGVDAIFIEALRDAAQMEAACKRFAARVPLLANMVEGGQTPIRS
                     ATELGALGFRIVIFPGGTARAAAHALQGYYASLAATGSTTAWRERMLDFDGLNALIGT
                     PAMLAAGKRYDAGE"
     gene            404..790
                     /locus_tag="VIS73_00315"
     CDS             404..790
                     /locus_tag="VIS73_00315"
                     /inference="COORDINATES: protein motif:HMM:NF040437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6481 family protein"
                     /protein_id="HEY5761626.1"
                     /translation="MDDMPSKEPGLAERLQTATEAKKAQLLKRHATALAAGAQSAERQ
                     AARVETAQARKTRMAERKDSSRARNEQKAAQRAAQQALQAQAAAQEQVRKEAERVAKA
                     QADAALKKEQKAARDSKYAARKARQK"
     gene            complement(1383..2090)
                     /locus_tag="VIS73_00320"
     CDS             complement(1383..2090)
                     /locus_tag="VIS73_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY5761627.1"
                     /translation="MSRTKPPPAVNRLLAALPRVDARRFQANSSPVVLTVGDVLSDIG
                     QPIRHVYFPTSCLISQETSVAGRSSLEVGLIGDEGMLGISLALGIGVSPLGAVVRSTG
                     SSLRMDNASFRRELERSKALRRVLNRYVHVLMGQFAQAAACTRFHVLEARLARLLLMT
                     NDRAHSDTFHVTHEVLAHMLGVRRVGVTKAATSLQERDLIRYRRGNIEILDRPGLEAA
                     SCACYATDTASYAQMFN"
     gene            2336..3199
                     /locus_tag="VIS73_00325"
     CDS             2336..3199
                     /locus_tag="VIS73_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY5761628.1"
                     /translation="MIAHQIDSPCRIGSLTQIHAGTRRNTVEPAYSEVDSRSKNETGT
                     TMKTSLIKKQLGLAISCALTLGFGSGGAFAQEDPMHRGFVQDPRGSIVKSGSGLCWHT
                     GAGPAPRSTPECDPNYAPQPVAKVERPMPPVVAAAPAPAPTPPPPPIALVQPAAEVVR
                     QRVTFDADALFDFDKSSLRPAGQLALDGFVGKLRDIEPQTITTVGHADRLGSSAYNQR
                     LSEQRVAAVKTYLVGKGVDPQRIRTEGRGESQPITKSGECVGAQSASVIACLQPDRRV
                     EIEVVGSRVVR"
     gene            3274..3495
                     /locus_tag="VIS73_00330"
     CDS             3274..3495
                     /locus_tag="VIS73_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEY5761629.1"
                     /translation="MNTMNRFAVGTVAAAMLLGLGACAGMSRQDQNTVTGAGIGAIGG
                     AILTGGSAVGTVGGAAVGGIIGHEVDKKR"
     gene            3586..3900
                     /locus_tag="VIS73_00335"
     CDS             3586..3900
                     /locus_tag="VIS73_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY5761630.1"
                     /translation="MTKSGKYLSALILALTLTSVVGCASTTRHEGTGEYLDDTVLTTK
                     VKAAVFNDPTLKSAEVNVETFKGVVQLSGFVSSQMAANKAVELARGVSGVKSVKNDMR
                     VK"
     gene            3937..>4770
                     /locus_tag="VIS73_00340"
     CDS             3937..>4770
                     /locus_tag="VIS73_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY5761631.1"
                     /translation="MTTPAPGLAAAATGSDHPVGDQTAAEAKPGASIPTVRLQVPRRG
                     LAVIVLATVAVVFALEWAQSLVISLLLGILFAYTLNPLVAWLERIRIPRVVGSSAVMA
                     GVVCVFALGAYSLQGQMQTILDRLPEAVSKLSASLASPRGGQPSTMQKVQTAATEIEK
                     ATSQAPETDPKPTKKATRVVVEPPSFKLGNYLWTNSLGVAALLGQTAMVLFLTLFLLI
                     SGDTFKRKLVRLTGPSLTHRKITVHVLDDINESIQRYMLMLLTTNVLVALLTWIVLRL
                     IG"
BASE COUNT          927 a         1546 c         1554 g          743 t
ORIGIN      
        1 gtgcggcgtc gacgcgatct tcatcgaggc gctgcgcgac gccgcgcaaa tggaggctgc
       61 ctgcaagcgt ttcgctgccc gggtgccgct gctggccaac atggtcgagg gcgggcaaac
      121 gccgatcagg tcggcgaccg agctcggggc gctcggcttt cgcatcgtga tctttcctgg
      181 cggcaccgcg cgcgccgccg cgcacgcgct gcagggctac tacgccagcc tggcggccac
      241 cggcagcacc accgcctggc gcgaacgcat gctcgacttc gacggcctga acgcgctcat
      301 tggcacaccc gcgatgctgg ccgccggaaa gcgctacgac gccggcgaat gaacccgcgc
      361 tgtcgcctgc cggtgcaggc agcgaagcgg ccgttcagat aagatggacg acatgccgag
      421 caaggaacca ggtctggcgg aacgcctgca aacagccacc gaggcgaaga aagcccagtt
      481 gctcaagagg cacgcgaccg cgctggcggc tggcgcccag tccgccgagc gacaggcggc
      541 acgggtggaa acggcccagg cgcgcaagac ccgcatggca gaacgcaagg actccagccg
      601 ggcgcgcaac gaacagaaag cggcgcaacg cgcggcccag caagcgctgc aagcccaagc
      661 cgcggcgcaa gaacaagtgc gcaaggaagc cgaacgcgtg gccaaggcgc aggccgatgc
      721 cgctctcaag aaggagcaaa aggccgcacg cgactcgaag tacgccgcgc gcaaagcgcg
      781 gcagaaatag ccccggagag ccccagggca ccaagccgcg ccacaacgca cccccgggcg
      841 gcaacgcgat tcagcgcagt gggccgaccc gcttcaggaa cgaaaacggt caggaaacgc
      901 gctattttgc gggaccggca cgcttcttct tcgcgtcttc cctgccaagc gcctccttgg
      961 tcggcttgac cttcttcacc accggcccgc caacgcctcc ttcgccagcc gcttggcgtt
     1021 cttggccttg agagcgagag tcttcttgtt cttctggtgc tgccgcctgg ctttgagcat
     1081 tgacgcgtga ctcatcttca ctccagtgga gatacgatcg aatttggcct cagtctgatc
     1141 gggttttgca cgccaaggaa cagaatgtct tctcagcgag cacttgccgt ttcccgcgca
     1201 ttgcagcctt cccgatgaag tcacgcgggc aagcgcaaga agaccgccga agcgggccgt
     1261 gcgacgcgag gtcgtcctgt ggtgcgctgt gccgccttgc tacgccgagg ccagcgaggc
     1321 cggtcgtcag aggtgcgagg gtcgcgaggg cttgcgcctt cagacccttc agacgaaagc
     1381 cgtcaattga acatctgggc gtatgacgcg gtatcggtcg cgtagcaggc gcacgacgcg
     1441 gcctcgagcc cggggcgatc gagaatctcg atgttgccgc ggcgataccg gatcaggtcg
     1501 cgctcctgca gcgacgtagc ggccttcgtg actccgacgc ggcgcacacc cagcatgtgc
     1561 gccagaacct cgtgcgtgac gtgaaatgtg tccgaatgag cgcggtcatt ggtcatcaga
     1621 agcagccgcg cgagtcgtgc ctcgagcacg tggaaccggg tgcatgcggc ggcctgcgcg
     1681 aactgtccca tcagcacgtg tacgtagcga ttgagcacgc gccgcagagc cttgctgcgc
     1741 tcgagttccc tgcgaaacga tgcgttgtcc atgcgcagcg acgagccggt ggaccggacc
     1801 acggcgccca gcggcgagac gccgatcccg agggcgagtg aaatgccgag catgccttcg
     1861 tcgccgatca acccgacctc gagactggat cttccggcga ccgacgtctc ctgcgagatc
     1921 aggcagctcg ttggaaagta gacgtggcgg atcggttgtc cgatgtcgga cagcacatcg
     1981 ccaacagtga gcaccaccgg cgaggaattc gcctgaaacc tccgggcatc cacgcggggc
     2041 aaggcagcca gaaggcggtt gacggcggga ggcggtttgg tgcgcgacac gcgaactcca
     2101 gttgctcgag caatttccgc acgcactgcg gaatattgat aacgatccgc cgatccagcg
     2161 gattccccgc ccggaatgcc cccggactcg acaccggcac tgtgaaccgt agcgcgacgc
     2221 ctgtctgggc gctggcgcac ataaggcaca gcgcgagttc cggcgtctcg ccgggatcgt
     2281 ccgaccgcac atcgttatgt gcgcagccgt acagacgcgc ggacgattca tcgcgatgat
     2341 tgcccatcag atcgattccc cgtgccgaat cggcagcttg actcaaattc atgcaggcac
     2401 gcgtcgaaac actgtcgaac ccgcgtattc ggaggtcgac agccgaagca aaaacgaaac
     2461 tggaacaacg atgaaaacgt cactaatcaa gaaacaactc ggactcgcga tctcgtgcgc
     2521 cttgacgctg ggcttcggat cgggcggtgc attcgcgcaa gaagatccca tgcaccgtgg
     2581 ctttgtccag gacccgcgcg gctcgatcgt caagagcggt tccgggctct gctggcacac
     2641 cggcgccggc cccgcgccgc ggtcgacgcc ggagtgcgac ccgaactacg cgccgcaacc
     2701 ggtggccaag gtcgagcggc ccatgccgcc cgtcgtcgcc gcagcgccgg cgccggcgcc
     2761 gaccccaccg ccaccgccga tcgcactggt gcagcccgct gcagaagtcg tcaggcagcg
     2821 cgtgaccttc gacgccgatg cgctgttcga cttcgacaag tcgtccctgc gcccggctgg
     2881 acagctcgca ctggacgggt tcgtcggcaa gctccgtgac atcgagccgc agacgatcac
     2941 gacggtcggc catgcggatc gcctgggctc ttccgcctac aaccaacgcc tgtccgaaca
     3001 gcgggtggcc gcggtcaaga cctatctggt cggcaaaggt gtcgatccgc agcggatccg
     3061 aaccgagggc aggggcgagt cgcagccaat caccaagtcc ggcgaatgcg tcggtgcgca
     3121 aagcgccagc gtgatcgcat gcctgcagcc cgaccggcgt gtcgagatcg aagtggtggg
     3181 cagccgggtc gtcagatagc agatagacaa gacccgcccg cactgcgcgc caatgaagta
     3241 cctgaagcaa aacgcaaacc aagaggcaga tccatgaaca cgatgaacag attcgcggtc
     3301 ggtaccgtcg cggcagcgat gctgctcgga ctcggcgcct gcgccgggat gtccaggcaa
     3361 gaccaaaaca ccgtgaccgg cgcaggcatc ggcgcgatcg ggggcgccat cctcaccggc
     3421 ggcagcgcgg tcggcaccgt cggtggcgcg gcagtcggcg gaatcatcgg ccacgaggtc
     3481 gacaagaagc gatgacggta aatcctggct ccgcgacggt gcgcgcgcgc acggccggtg
     3541 ccaggcagaa atgaaaaagt cccaccacat actggagatg caatcatgac gaaatctggc
     3601 aaataccttt ccgcacttat tctggctttg accctgacgt ccgtcgtggg gtgtgcgtcc
     3661 acgacacggc acgagggaac gggcgaatac ctggacgaca cagtcctgac gacgaaggtc
     3721 aaggcggccg tgttcaacga cccgacgctg aaatcggcag aggtcaatgt cgagaccttc
     3781 aagggcgtgg ttcagctgag cggcttcgtg agttcgcaga tggcggccaa caaggcggtc
     3841 gagctggcgc gcggcgtgag cggcgtgaag tcggtcaaga acgacatgcg ggtcaagtaa
     3901 gcgtaggacg ggaggctggc ccacaggttt ttcgaggtga cgacgcccgc tcccggcctc
     3961 gcagccgcgg caaccgggtc cgaccatccg gttggcgatc agaccgctgc ggaggcaaag
     4021 ccgggcgcct cgatcccgac ggtgcggcta caggttccga ggcgcggtct ggcggtcatc
     4081 gtcctcgcga cggtggccgt cgtctttgcg ttggagtggg cgcagagtct cgtcatctcg
     4141 ctgctgttgg ggatcctctt cgcctacacg ctcaacccgc tcgtggcctg gctcgagcgg
     4201 atcaggatcc cgcgagtcgt ggggtccagc gcggtgatgg cgggtgtcgt ctgcgtgttc
     4261 gcgctgggcg cctattcgct gcaggggcag atgcagacga ttctcgatcg cttgccggag
     4321 gcggtgagca agttgtccgc gagtctcgcc agcccccgcg gtggccagcc cagcacgatg
     4381 cagaaggtgc agaccgcggc gaccgaaatc gaaaaggcga ccagccaggc gccggaaacg
     4441 gatcccaagc ccacgaagaa ggcgacgcgc gtcgtcgtcg agccgcctag cttcaagctc
     4501 gggaactatc tctggaccaa ttcgctcggc gtcgcagctc tgctcggcca gaccgcgatg
     4561 gtgctgttcc tgaccctctt tctgctgata tcgggtgaca ccttcaagcg aaagctggtc
     4621 aggctgaccg ggccctcgct aacgcatcgc aagatcactg ttcacgtact cgacgacatc
     4681 aacgaatcga tacagcgcta catgctgatg ttgttgacca ccaacgtgct ggtggcgctg
     4741 ctcacctgga tcgtgctgcg cctgatcggc
//
LOCUS       DATDAA010000009         8363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_489315,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000009 DATDAA010000000
VERSION     DATDAA010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489315"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /gene="guaA"
                     /locus_tag="VIS73_00345"
     CDS             complement(<1..815)
                     /gene="guaA"
                     /locus_tag="VIS73_00345"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY5761632.1"
                     /translation="MSHQKILILDFGSQLTQLIARRVREQQVYCELHPFDVSETFIRE
                     FAPKGIILSGGPNSVYEATDWKAPPVVFELGVPVLGICYGMQTMAEQLGGKVESSGKR
                     EFGYAEIRARGHSPLFKGIQDRSNADGHGLLDVWMSHGDKVVELPTGFRVIASNEATP
                     IAAMADEARGYYGVQFHPEVTHTLKGREIISRFVHEVCGCGQDWNMPDYVNEAVEKVR
                     AQVGSEEVILGLSGGVDSSVVAALLHRAIGDQLTCVFVDNGLLRLNEAQQVMET"
     gene            complement(881..2341)
                     /gene="guaB"
                     /locus_tag="VIS73_00350"
     CDS             complement(881..2341)
                     /gene="guaB"
                     /locus_tag="VIS73_00350"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY5761633.1"
                     /translation="MQVIKKALTFDDILLVPAHSNVLPRDVSLKSRLTRNISLNMPLL
                     SAAMDTVTEARLAIAVAQEGGIGILHKNLSPKAQAAEVVKVKRFESGVVKDPITIPPG
                     MSVRDALTLTRSNRISGLPVVDGKKVVGIVTNRDLRFETNLDQPIQSIMTPRDRLVTV
                     KEGASVDEAKALIHRHRLERVLVIDDDYELKGLITVKDILKSTEHPNACKDESGRLRV
                     GAAIGVGVGTDERATLLIEAGVDVLIVDTAHGHSEGVLQRVRWVKKHFPHVEVIGGNI
                     ATGEAARALMDCGADGVKVGIGPGSICTTRIVAGVGVPQITAIDNVATALAGTGIPLI
                     ADGGIRYSGDIAKAIAAGANSVMLGGLFAGTEEAPGEVELYQGRSYKSYRGMGSMGAM
                     AAGSSDRYFQDNNANADKFVPEGIEGRVPYKGPVTAVIHQLMGGLRSSMGYLGCESID
                     AMHERARFVEITSAGVRESHVHDVQITKEAPNYRMD"
     gene            2413..2910
                     /locus_tag="VIS73_00355"
     CDS             2413..2910
                     /locus_tag="VIS73_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY5761634.1"
                     /translation="MNFPLRKAWISSVALVIAGTVFAPNADAQVYKYRDPATGKTIMT
                     DNPPAGAARQRPSATRNNDETGEAEAPGDGTAPQKTSGIDPRLEERKREEEAKARAEQ
                     EAANAKLEEQKRQYCADLDRNIATLESGQRIAQMNAAGEREFMTDDKRSAEISRMRAE
                     QAKCE"
     gene            complement(2965..3291)
                     /locus_tag="VIS73_00360"
     CDS             complement(2965..3291)
                     /locus_tag="VIS73_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEY5761635.1"
                     /translation="MSEQLSVEVIYALPEKQVLIPLRMPEGSTVRDAVAASGLDLEYP
                     EVDFSALKVGVWAKLVKPDTPLRDRDRVEIYRPLIADPKEVRKQRAAAGKVMKKGAAE
                     AGEGEA"
     gene            complement(3284..3727)
                     /locus_tag="VIS73_00365"
     CDS             complement(3284..3727)
                     /locus_tag="VIS73_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEY5761636.1"
                     /translation="MASVEKTVLIERSPAQMFELVDNVERYPEFLPWCNRTELKARSD
                     IETVATLHINFHAVKSHFTTRNGKTYPSEMIIRLVDGPFRRLEGKWHFKALGESACKI
                     EFSLHYEFSSRLFEKVIGPVFTHIAGTFVDAFVKRAEQIYGAQNV"
     gene            3787..4236
                     /gene="smpB"
                     /locus_tag="VIS73_00370"
     CDS             3787..4236
                     /gene="smpB"
                     /locus_tag="VIS73_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY5761637.1"
                     /translation="MTISQNKKASHDYFIEERFEAGIVLEGWEVKAIRSGRAQINEAH
                     VIVKGEEIWIIGMHITPLISASTHVKPDATRTRKLLLHRNQISRLIGKVERAGFTLVP
                     LDLHFMRGRIKVAIGLAKGKKQFDKRADLKDREWKREQGRLLKSKAR"
     gene            4263..4907
                     /locus_tag="VIS73_00375"
     CDS             4263..4907
                     /locus_tag="VIS73_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEY5761638.1"
                     /translation="MIITADLFLYWVGLAAVAAMAAAGVLEAERNRTDLVGAIFIAMA
                     SALAGGTVRDVLLDRQVFWVADQTYLLTAAGAGVLIFFVIRERPVPARLFLYPDAVGL
                     ALFTVFGTQAALQWNTPWFVATLMGVTTAVFGGIARDVLCNRMPLVMQRGELYATAAW
                     LGSLVVVGISEFGGDRITAGWCGMAVVLVVRLAAMHFALRLPVIEKDKPNPGSE"
     gene            complement(4924..7296)
                     /gene="ppsA"
                     /locus_tag="VIS73_00380"
     CDS             complement(4924..7296)
                     /gene="ppsA"
                     /locus_tag="VIS73_00380"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEY5761639.1"
                     /translation="MNRYVIDFRELRMTDVEQVGGKNASLGEMISQLSKTAVRVPGGF
                     ATTAEAYREFLAFGGLHDRIHQTLDQLNIEDVDALARAGAEIRAWIIDQPFPEEFKAA
                     IAECYAQLTADTGSEASFAVRSSATAEDLPDASFAGQQETFLNISGIDNILHAIKEVF
                     ASLYNDRAIAYREHKGFRHAEVALSAGVQRMVRSDKGSAGVMFTLDTESGFRDAVFVT
                     ASYGLGETVVQGAVNPDEFYVHKPMLEAGKKAIVRRTLGSKLIKMEFARENTAGKSTH
                     TIDVPEVDRHRFSINDADVEELARYAMIIERHYQRPMDIEWGKDGIDGKLYILQARPE
                     TVKSQEGHERLQKFRLKSFSKVLASGRAIGQKIGVGPVRIVADPTEMNQVRPGDILVA
                     DMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAIVGCGDATDKLNEGDLVTV
                     SCAEGDTGHVYRGKLDFEVSTREVGTMPEVPVKIMMNVGNPELAFEFSQLPNAGVGLA
                     RVEFVINNVIGIHPKAILEIERLPASKREEILRRARGYASPVEFFVDKLAEGVSTIAA
                     AFWPKPVIVRMSDFKSNEYRKLLGGELYEPEEENPMLGFRGASRYVAQDFRDCFALEC
                     RALKKVREEQGLTNVELMIPFVRTVDEGKRVVELLAEHGLRKGENGLKVIMMCEIPSN
                     ALLAEQFLEVFDGFSIGSNDLTQLTLGLDRDSGLVANLFDERDPAVKALLALAIRGAR
                     AKGKYVGICGQGPSDHPDLAEWLMDEGISSISLNPDTVVDTWTRLGSHSR"
     gene            complement(7360..8121)
                     /locus_tag="VIS73_00385"
     CDS             complement(7360..8121)
                     /locus_tag="VIS73_00385"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY5761640.1"
                     /translation="MVTKSELSIAVTRVPDAVARELERRILDGEFGPGDRLPPERQLA
                     EALGVSRASLREAIQKLTARGWLRSRQGEGTFVTEQLQASFADPWQQLIEQHPSVRED
                     LLEFRHMLEAKAAALAAQRASEEDIETIRGAYLRMENAYAADDLQSLVQADVAFHQAL
                     VQAAHNAVFSQLSSSLFRVMREHIELNLEILMRVPSAREQLGEQHRAVWHAVLARDSR
                     AASAAMESHIDYVRQSLSENLRKQRSRGRLSPQDA"
BASE COUNT         1601 a         2570 c         2553 g         1639 t
ORIGIN      
        1 gtctccatca cctgctgcgc ttcgttaagg cgcagcaggc cgttgtcgac gaacacgcag
       61 gtgagttgat cgccgatcgc ccgatgcagc aaggccgcga ccaccgatga atcgacgccg
      121 cccgagaggc cgaggatgac ctcctcgctg ccaacctgag cacggacctt ctcgaccgcc
      181 tcgttgacgt agtcgggcat gttccagtct tgcccgcagc cgcagacctc gtggacgaag
      241 cgcgagatga tctcgcgacc cttaagtgtg tgcgtgactt cggggtggaa ctgcacgccg
      301 taatagccgc gcgcctcgtc ggccattgca gcgatcggcg tagcttcatt gctggcgatg
      361 acgcggaaac cggtgggcaa ttcgacgacc ttgtcgccat gactcatcca gacgtcgagc
      421 aatccatgtc cgtcggcatt gctgcggtct tggatcccct taaacagcgg cgaatgcccg
      481 cgcgcgcgga tctcggcata accgaactcg cgcttacccg agctttcgac cttgccacca
      541 agctgctcgg ccattgtctg catgccgtaa cagatgccaa ggaccggtac gcccagctcg
      601 aaaacaaccg gcggtgcctt ccagtcggtc gcttcgtaga ccgagttagg tccccccgaa
      661 aggatgattc ccttcggcgc gaactcgcgg atgaacgtct cgctcacatc gaacggatga
      721 agctcgcaat agacctgctg ttcgcgtacg cggcgtgcaa tcagttgcgt cagctgcgat
      781 ccgaaatcga ggatgaggat tttctggtga ctcacggcgg ctccctgtct tcaacgacaa
      841 agggcggccg ggccgccctt cccctgcgcc aaacgcgctc ttaatccatc cggtagttcg
      901 gcgcttcctt ggtaatctgc acgtcgtgaa cgtgcgattc ccgcacgccc gccgaggtga
      961 tctcgacgaa tcttgcccgc tcgtgcatcg cgtcgatcga ctcgcaaccc agatatccca
     1021 tcgacgaacg tagcccaccc atcaactggt gaatgacggc ggtaaccggt cccttgtacg
     1081 gcacacggcc ttcaatgcct tcgggaacga acttgtcggc gttggcgttg ttgtcctgga
     1141 aataacggtc agagctgccg gctgccatcg cccccatcga gcccatcccg cgataagact
     1201 tgtaggaacg cccctggtag agctcgacct cgcccggtgc ctcctcggtt ccggcgaaca
     1261 gcccgccgag catcaccgag tttgcgcccg cggcaattgc cttggcgata tcgcccgagt
     1321 agcgaatccc gccatcggca atcaggggga taccggtgcc agcgagtgcc gtcgcaacat
     1381 tgtcgatcgc ggtgatctgc ggcacaccaa cgccggcgac gattcgcgtc gtgcagatcg
     1441 agccaggtcc gatgccgacc ttgacgccat ctgcgccgca atccatcaat gcgcgagcag
     1501 cctcgccggt ggcgatattg ccgccgatga cttcaacgtg cgggaagtgt ttcttgaccc
     1561 agcggacgcg ttggagaaca ccttcggagt ggccgtgggc ggtatcgacg atcagcacat
     1621 cgacgcctgc ttcgattagc agcgtggcgc gctcgtcggt gccgacgccg acaccgatcg
     1681 ccgcgccaac gcgaagccga cccgactcgt ccttgcaggc attgggatgt tcggtcgact
     1741 taagaatgtc cttgacggtg attagtccct tgagttcgta atcgtcgtcg attacaagca
     1801 cgcgctctag gcggtgccga tgtatcagcg ccttggcctc gtcgacgctg gccccctcct
     1861 tgaccgtgac cagacgatcg cgcggcgtca tgatcgactg gatcggctgg tcgagattgg
     1921 tttcgaatcg caggtctcgg ttggtgacga tgccgacgac cttcttgcca tcgacgaccg
     1981 gtaatcccga gatgcggttg gagcgcgtca gcgtcagcgc atcccgcacc gacatgccgg
     2041 gcggaatcgt aatcggatcc ttcaccacgc ctgattcgaa acgcttgacc ttgaccacct
     2101 cagcagcctg cgccttcggg ctcaggttct tgtgcagaat gccgattccg ccttcctgcg
     2161 cgaccgcaat ggccagacgc gcttcggtca ccgtatccat ggcagccgag agcagtggca
     2221 tgttgagcga aatgttgcgg gtgagtctgg acttgaggct gacgtcgcgc ggaaggacgt
     2281 ttgaatgggc tggaacgaga agaatgtcgt cgaaggtcag cgccttctta atgacttgca
     2341 tggcctttta ccctaggtca caaaaacgta ttatacgggc ttcgccgaat cccggaaaag
     2401 gcacgcgcca ccatgaattt cccgctacgc aaagcctgga tcagcagcgt cgcactggtc
     2461 attgccggaa ccgtattcgc gcccaatgcc gacgcgcagg tctacaaata ccgagatccg
     2521 gcgaccggca agaccatcat gaccgacaat ccgcctgccg gcgcggcgcg gcaacgacca
     2581 tccgctacgc gcaataacga cgaaacaggt gaagccgaag ccccgggcga tggcacggcg
     2641 ccgcagaaga catcgggtat cgatccccgc cttgaagagc gaaaacgcga ggaagaggcg
     2701 aaggcgcgtg cggaacaaga ggcagcgaac gcgaagctcg aagaacagaa acgccagtat
     2761 tgcgccgacc tcgatcggaa catcgcaacg ctcgaatcgg gccagcggat cgcgcagatg
     2821 aacgctgccg gcgagcgcga attcatgacc gacgacaagc gcagcgccga gatcagccgg
     2881 atgcgcgccg agcaggccaa atgcgagtag caggcgtgta gtccctagcg gtcgaatcgc
     2941 gccgagctga agccagcgcg tgtattacgc ctcgccctcg cccgcttccg ctgctccctt
     3001 cttcattacc ttgcctgccg ccgcgcgttg cttgcgcact tctttggggt cggcaatcag
     3061 cggtcggtag atttccacgc gatcgcggtc gcgcagcggc gtatcgggct tgacgagctt
     3121 ggcccagacg ccgactttca gggcgctgaa atcgacctca gggtattcca gatccagacc
     3181 cgaggcagca acggcatctc gaaccgttga cccttcaggc atgcgaagcg gaatcaggac
     3241 ctgcttttcc ggcagcgcgt agattacttc gacgctgagt tgctcagaca ttctgcgctc
     3301 catagatctg ctccgcgcgc ttgacgaagg cgtcgacaaa ggtacctgca atatgcgtga
     3361 agactggtcc gatgaccttc tcgaacagac ggctggaaaa ctcgtaatgc aggctgaact
     3421 cgatcttgca ggccgattcg ccaagtgcct tgaaatgcca tttcccttcc agtcggcgaa
     3481 aaggaccatc gacgagacga atgatcatct cgctaggata ggtcttgccg ttccgggtcg
     3541 tgaagtgcga cttgaccgca tggaaattga tgtgcagggt cgccacggtt tcgatgtcgc
     3601 tgcgggcctt caactcggtc cgattgcacc aaggaaggaa ctcgggatag cgctcgacgt
     3661 tgtcgacgag ttcgaacatc tgtgcgggac tccgctcaat caggacagtt ttttctacgg
     3721 atgccatcag gcgtcgtcgt gcggagtcgc tagaatccgc attctatcga aagcacagct
     3781 tttccaatga cgatttccca gaacaagaag gccagccacg actatttcat cgaagaacgc
     3841 ttcgaagccg gaatcgtgct ggaaggctgg gaggtcaagg cgattcgctc tggtcgggcg
     3901 cagatcaatg aagcgcacgt gatcgtcaag ggcgaagaaa tttggatcat cggcatgcac
     3961 atcacgccgc tgatttcggc ttcaacgcac gtcaagcccg acgcgacgag gacccgcaag
     4021 ctgttgttgc accggaacca gatcagccga ctgatcggca aggtcgagcg cgcgggattt
     4081 acgctggtgc cgctcgacct gcatttcatg cgcggccgga tcaaggtggc gatcggactg
     4141 gctaaaggca agaagcagtt cgacaagcgt gccgacctga aggatcgcga atggaaacgt
     4201 gagcagggtc gcctgctcaa gtccaaggcg cgttgatcgc ggcctgagcc gcggcgtttg
     4261 cgatgatcat tactgccgat ctttttcttt attgggtagg ccttgccgcc gttgcggcga
     4321 tggccgcggc tggcgtgctt gaagccgagc gcaatcgcac cgacctggtc ggtgcgatct
     4381 ttatcgcaat ggccagcgcc ctcgcgggtg gcaccgtccg cgacgtgctg ctcgaccggc
     4441 aggtcttctg ggttgctgac cagacctacc tactgacggc tgccggcgct ggcgttctga
     4501 tcttcttcgt gatccgcgag cggccggtgc cggcacggct gtttctgtat ccggatgcgg
     4561 ttggccttgc actgtttacc gtattcggca ctcaggcggc actgcagtgg aatacgccgt
     4621 ggttcgtcgc gacgctgatg ggcgtaacta ccgcggtgtt cggcggcatc gcacgcgatg
     4681 tgctgtgcaa ccggatgccg ctggtcatgc agcgcggcga gctctacgca acggctgcct
     4741 ggctgggttc gctggtggtc gtgggcatat ccgaattcgg cggcgatcga atcactgccg
     4801 gctggtgcgg catggcggtc gtgctcgtgg ttcgcctggc ggcaatgcat ttcgcgctgc
     4861 gcctgccggt catcgagaag gataagccga accccggcag cgaataaaca ccgccggaaa
     4921 aagtcagcgg ctatggcttc ccagccgcgt ccaggtgtcg acgacggtgt cggggttaag
     4981 cgaaatgctg ctgatgccct cgtccattag ccactcggcg agatcggggt gatcagacgg
     5041 gccctgcccg cagatgccga catacttgcc tttggcgcgc gcgccgcgga tcgccaacgc
     5101 caacagcgcc ttgaccgcgg ggtcgcgttc atcgaacaga ttggcgacca ggccggaatc
     5161 gcgatcgagg cccagcgtca gctgagtcaa atcgttcgag ccgatcgaga aaccatcgaa
     5221 cacctcgagg aactgctcgg ccagcagcgc attggacgga atctcgcaca tcatgatgac
     5281 cttcagcccg ttctcgccct tgcgcagccc atgttcagcg aggagttcga cgacccgctt
     5341 gccttcatcg acggtgcgaa cgaagggaat catcagttcg acgttggtca ggccctgctc
     5401 ctcacggacc ttcttcagcg ctcggcattc gagcgcgaag cagtcgcgga agtcctgcgc
     5461 cacgtaacgt gaagcgccgc gaaatccgag catcgggttc tcttcttcgg gttcgtaaag
     5521 ctcgccgccg agaagcttgc ggtattcgtt cgacttgaaa tccgacatgc ggacgatcac
     5581 cggcttcggc cagaaggctg cggcgatcgt cgacacgcct tcggcgagct tgtcgacgaa
     5641 gaactcgacc gggctcgcgt aaccgcgggc gcgacgcagg atttcctcgc gcttcgaggc
     5701 cggcaggcgc tcgatctcga ggatcgcctt cgggtgaatg ccgatgacgt tgttgatgac
     5761 gaattcgacg cgcgccaggc cgacgccggc gttgggaagc tgagagaact cgaatgcgag
     5821 ttcggggttg ccgacgttca tcatgatctt gaccggcact tccggcatcg ttccgacctc
     5881 acgcgtgctg acctcgaagt cgagcttgcc gcgatatacg tggccggtat cgccctcggc
     5941 acaagagacc gttaccaggt cgccctcgtt aagcttatcg gtcgcgtcgc cgcaaccgac
     6001 gatagccgga atgccgagtt cgcgggcgat gatcgcggcg tggcaggtcc ggccaccccg
     6061 attggtcacg atcgccgagg cgcgcttcat caccggctcc cagttggggt cggtcatgtc
     6121 ggcgacgagg atgtcgcccg ggcggacctg attcatctcg gttgggtcgg caacgatgcg
     6181 gaccggcccg acgccgatct tctggccgat cgcgcggccc gaggcgagga ccttcgagaa
     6241 gctcttgagg cggaatttct gcaggcgctc gtggccctcc tgcgacttca ccgtctcggg
     6301 gcgcgcctga aggatgtaga gcttgccatc aatgccgtcc ttgccccatt cgatgtccat
     6361 cggtcgctgg tagtggcgct cgatgatcat cgcgtagcgc gccagctcct cgacatcggc
     6421 atcgttgatc gagaatcgat ggcgatcgac ctcaggcacg tcaatcgtgt gggtcgattt
     6481 gccggccgta ttctcgcgtg cgaattccat cttgatcagc ttcgaaccga gcgtgcggcg
     6541 aacgatggcc ttcttgcccg cttcgagcat gggcttgtgc acgtagaact cgtcgggatt
     6601 gactgcaccc tggacgactg tctcgcccag accgtatgag gccgtgacga agaccgcgtc
     6661 acggaatccg gattcggtat cgagcgtaaa catcacgccg gccgaaccct tgtccgaacg
     6721 aaccatccgc tgcacgcctg ccgacagcgc gacttcggcg tggcgaaaac ccttgtgctc
     6781 gcggtaggcg attgcacgat cgttgtagag cgaagcaaag acctccttga ttgcatggag
     6841 gatgttgtcg atgccgctga tattgaggaa ggtctcctgt tgcccggcaa aggaggcatc
     6901 gggcaggtcc tcagccgttg ccgacgagcg gaccgcgaag gatgcttcgc taccggtgtc
     6961 ggccgtcagc tgtgcatagc attcggcgat tgccgccttg aattcctcgg gaaaaggctg
     7021 gtcgatgatc cacgcgcgaa tctccgcccc ggctctggcc aaggcatcga catcttcgat
     7081 attcaactgg tcaagcgtct ggtggattcg atcatgcagg ccaccgaagg ccaggaactc
     7141 gcgataggcc tcggccgttg tcgcaaatcc gccaggtacg cgcactgcgg tcttggacag
     7201 ctggctgatc atttcgccca gagaggcgtt cttgccgccg acctgctcca catcggtcat
     7261 gcggagctca cggaaatcaa tgacgtagcg attcatggca acctgcgctg tcggaaaaag
     7321 gcgcgaggat agcatcgcac cgcgctgcct aagcgggctt catgcgtcct gcggtgacaa
     7381 tcttcctcgc gaacgctgtt tgcgcaggtt ctcgctgaga ctctggcgca cgtagtcgat
     7441 gtgcgactcc atcgccgcgc tcgccgcgcg tgagtcacgc gccagcaccg catgccagac
     7501 ggcacggtgc tgttcgccga gttgctcgcg ggcgctgggt acccgcatga ggatctcgag
     7561 gttcaattcg atgtgctcgc gcatgacgcg aaataggctg gaacttaact ggctgaacac
     7621 ggcgttgtgc gccgcctgta ccagggcctg atggaaggcc acgtcggcct ggacgagcga
     7681 ctgcaggtcg tcggcggcgt aggcgttttc catgcgcaga taggcgccgc ggatcgtctc
     7741 gatatcctct tccgaggcgc gctgcgcggc aagggctgcc gccttggctt ccagcatgtg
     7801 ccgaaactcc agcaaatcct cgcgaaccga cgggtgctgc tcgatcagct gttgccaggg
     7861 atcggcaaag cttgcctgga gctgctcggt aacaaaggtg ccctcgccct gccgcgaccg
     7921 cagccagccg cgcgcggtca gcttctggat ggcctcgcgc agcgaagcgc gggaaacacc
     7981 gagcgcctcg gcaagctgcc gctcgggcgg cagccggtcg ccgggaccga attcaccatc
     8041 gagaatccgc cgctccagtt cgcgcgccac ggcatccgga acccgcgtca cggcgatcga
     8101 gagctcgctc ttggtaacca tgtggcttct ccgtaggtaa ggcgtaggta aggtggcgcc
     8161 gggcttgctg gattaaatgg ccgaaataat tggtcggacc aatgatatat gccgaatcct
     8221 gtgacagcaa ccccttggtg gccccgctcc tggaattggc tccctacgag gaaaggccaa
     8281 tcgatgtttg atgttttgcg cggtgcagca agacatctcg gtggataacc cctattgaca
     8341 atcgattccc taccgcgtaa att
//
LOCUS       DATDAA010000010         2339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_210062,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000010 DATDAA010000000
VERSION     DATDAA010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210062"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..>2339)
                     /locus_tag="VIS73_00390"
     CDS             complement(177..>2339)
                     /locus_tag="VIS73_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEY5761641.1"
                     /translation="DWASPRNIGFSCVISSGIAADVDFGEILDYLVIDPTTQAIMLYV
                     EGIHEPRRFMSALRAASRAKPVIVMKAGRFAQGSRAAVSHTGALVGADDVFDAALNRA
                     GVVRVHDYSDFFAVAETLHTGVRTAGPRLAVVTNGGGPGVMAADFLIDRGLVLADLSK
                     STCRELDEQLADCWSHDNPVDVLGDASDDQYAAAVETCLGDDGVDCVLAIVVPQAMTD
                     PTTIAERIVGLKESAKKPVLTCWMGDESVQASRNLFREHGLPTYATPEAAVEAFAAAA
                     AYRANQELLLQVPQALGKRAEPDREGAQLIIQNALAQKRRVLDLVESKALLAAFGIPV
                     VRSIPAHDPSEALAIAEEFGFPVVMKIHSPDISHKTEVDGVRLGINGGRDVQTAFRQL
                     VETAEERRPDAKIRGVLIEPMWQAHAGRELMLGVINDEVFGPVISVGLGGTMVEVLRD
                     RAIGLPPLNRFLAKSMIAGTRASRFLDEFRGRPAANRRALENVILRLSDRVCELPWLE
                     ELDINPLVVDTEKAMALDARIVVRPVSPAAREYSHMAIHPYPSKLVDEHRLLDGTYIT
                     IRPIRPEDAETEREFVDGLSERSRYLRFMYSLKNITLQMVSRFTQIDYDREMALIALV
                     DADDGERQIAVGRYVMNPDGRSCEFAIVVADDWHGKGIATELLRRLIDIARDRRLEEM
                     VGIVLRENKGMIALAKELGFDQQRSPDDPGVVIMTLKL"
BASE COUNT          414 a          774 c          730 g          421 t
ORIGIN      
        1 ctcgagatgt acaaggcggc catcgatggg gatcgtgccg ctgcggtcgc ggcctaccag
       61 ggcatggtcg ccgcctgttt cgactgccat gcggcctacc gggagcgcgt agccgccgcc
      121 ctggtgacgg tcgacgagta agagacgctt tcagcctgct gcagcgaatc tcgaactcag
      181 agtttcagcg tcatgatcac gacgcccggg tcgtccggcg agcgctgttg gtcgaagccg
      241 agttccttag cgagcgcgat catgcccttg ttctcacgta gcacgatgcc gaccatctcc
      301 tcgaggcggc ggtctctcgc gatgtcgatg agccggcgca gcagttcggt cgcgatgccc
      361 ttgccgtgcc aatcgtcggc gacgacgata gcgaactcgc agctgcggcc atcggggttc
      421 atcacgtagc ggccgacagc aatctgccgc tcaccgtcgt cagcatccac cagcgcgatc
      481 aacgccatct cccggtcgta gtcgatctgt gtaaagcgcg acaccatctg cagggtgatg
      541 ttcttcaggg agtacatgaa acgcaggtag cgggagcgtt cggacagccc gtccacgaat
      601 tcacgctcgg tctcggcgtc ctccggccga atcgggcgta tcgtgatgta ggtaccgtcg
      661 agcagccggt gctcgtcgac gagcttgctc gggtaagggt gaatcgccat gtgcgagtat
      721 tcccgcgctg ccgggctcac gggcctgacc acgatgcgcg catcgagcgc catggccttc
      781 tcggtgtcga cgacgagcgg gttgatgtcg agttcctcga gccagggcaa ctcgcagacc
      841 ctgtcggaca gtcgcaagat gacgttttcc agtgcacggc ggttcgcagc cggccgaccg
      901 cggaattcgt ccaggaaacg cgatgcccgg gtgccagcga tcatgctctt cgcaagaaac
      961 cggttcaatg gcggcaggcc gatggcacga tcccgcaata cctcgaccat ggtgccgccg
     1021 aggccgacgc tgataacggg cccgaacact tcgtcgttga tcacgccgag catcagctca
     1081 cggcccgcgt gcgcctgcca catgggctcg ataagcacgc cgcgtatctt cgcatcgggc
     1141 cgccgctcct cggcggtttc cacgagttgc ctgaacgcag tctgcacgtc gcggccgccg
     1201 ttgatgccaa ggcgcacgcc gtcgacttcc gtcttgtgcg agatatccgg cgagtgaatc
     1261 ttcatcacca cggggaaacc gaactcctcg gcgatggcga gcgcctcgct gggatcgtgc
     1321 gcggggatac tgcgaaccac cgggatgccg aacgcggcca acagcgcctt cgactcgacc
     1381 aggtcgagca cgcggcgctt ttgcgccagc gcgttctgga tgatgagctg cgcaccttca
     1441 cgatccggct cggcacgctt gccgagcgct tgcggaacct gcagcagcag ttcctggttg
     1501 gcccggtacg cggctgcggc tgcaaacgcc tcgaccgccg cttccggcgt ggcgtaggtc
     1561 ggcaatccgt gctcgcgaaa caggtttcgg cttgcctgca cggactcgtc gcccatccag
     1621 cacgtcagga ccggtttctt cgcgctttcc ttcagcccga cgatgcgctc cgcgatcgtt
     1681 gtcgggtcgg tcatggcctg cggtaccacg atcgccagta cgcaatccac accgtcatcg
     1741 cccaggcagg tttccaccgc cgctgcatac tggtcgtcgc tcgcatcgcc gaggacatcg
     1801 acgggattat cgtgcgacca gcaatccgcc agctgctcgt cgagctcccg gcacgtcgac
     1861 ttggacaggt ccgcgagcac gaggccacgg tcgatgagaa agtcggccgc catcactccc
     1921 ggaccgccgc cattggtgac gacggcaagc ctcggcccgg ccgtccgcac gccggtatgc
     1981 agggtctctg ccaccgcgaa gaaatcggaa tagtcgtgga cgcgcacgac accggcacgg
     2041 ttcagtgctg catcgaagac atcgtcggca ccaacgagtg caccggtatg agacacggcc
     2101 gcgcggctgc cctgcgcgaa gcgccccgct ttcatgacga taacgggctt ggcgcgcgat
     2161 gcggcccgca aggcactcat gaaccggcgc ggttcgtgaa taccctcgac gtacagcatg
     2221 atcgcctgtg tcgtcggatc gatcacgagg taatcgagga tctcgccgaa gtcgacgtcg
     2281 gcagcaatgc cgcttgagat aacacaggaa aaccctatat tccgcgggct tgcccagtc
//
LOCUS       DATDAA010000011         7174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_175143,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000011 DATDAA010000000
VERSION     DATDAA010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175143"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VIS73_00395"
     CDS             complement(<1..777)
                     /locus_tag="VIS73_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY5761642.1"
                     /translation="MSEWLMHPGLWLIAAAFVLPLLSGHARAVLILAAPLVSLGLTWQ
                     LGDGVHWQLSWLGLELQPLVADKLTRLFATIFALMAFGGGLFALGQKSRIEVPAAFVY
                     AGSAIGVAFAGDLVTVFIFWELMAVGSTLVLWSAATEGAFRASKRYLAIHLFGGVVLF
                     AGITGHIVATGSTAFGVMQADSAANVLILIGFLINAGAPMISAWLPDAYPEASWSGTV
                     FLSAFTTKTAVYVLLRGFPGTEILIWLGIFMIFYGIVYALL"
     gene            complement(770..1048)
                     /locus_tag="VIS73_00400"
     CDS             complement(770..1048)
                     /locus_tag="VIS73_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761643.1"
                     /translation="MQTPEKTHWLDHPENVRKLWWGFLGVLALTVVAELVVALHPHFE
                     VESWFGFHAIFGFLACAAMIVGAKGLGLLLKRPDTYYDETDGGPARDE"
     gene            complement(1048..2511)
                     /locus_tag="VIS73_00405"
     CDS             complement(1048..2511)
                     /locus_tag="VIS73_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY5761644.1"
                     /translation="MSATLALAGGIVIPVAGALGIVACHRAPNLRETVTLVTAVLLFA
                     TVASLLPGVLAGDRPELSIVEILPGLALSFRLEPLGMLFALIASGLWIINSIYSIGYL
                     RGNDEKHQTRFYVCFALAIAAAMGIAFAGNAFTLFVFYEMLTLVTYPLVTHHGTDHAK
                     RGARVYLGVLLSTSILFLLPAIVFTWYLAGTTEFTPGGILPEGAGSLALGGLLALYMF
                     GIGKAALMPFHRWLPSAMVAPTPVSALLHAVAVVKAGVFSVVKVMIYVFGVDQLQLAG
                     TDWLVAVSGFTIVAASITALNADNLKRRLAYSTVSQLSYVVLAAALLAPLSMVAAILH
                     IAAHALGKITLFFAAGSIYTAAHKTEVSQLDGIGRRMPWTMAAFAIGALSMIGLPPAA
                     GFVSKWFLVSGAIAQAHWMALTVIAASTLLNAAYFMPIIYRAFFRPLSANDEAHPHGE
                     SPLPMVVALTLTALGTIALFFFPDVPLALAGAALGGI"
     gene            complement(2508..3971)
                     /locus_tag="VIS73_00410"
     CDS             complement(2508..3971)
                     /locus_tag="VIS73_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D family
                     protein"
                     /protein_id="HEY5761645.1"
                     /translation="MPALQVALPLLAATLAVLGRRAGFAFIVVLAASWAALVIAVLLW
                     LQVADGSVISYAMGNWRPPWGIEYRVDRLSAFILVLVSGMAAITLPYCRASVAREVRP
                     EQHYLFYAMMALCFAGLLGITITGDAFNVFVFLEVSSLSTYVLIALGRDRRALLASFQ
                     YLIMGTIGATFIVIGVGLLYLKTGTLNLADMSVRLQEMTSHRPVLAALAMLTVGISLK
                     LALFPLHQWLPNAYTYAPSAVSAFLAGTATKVSVYLLIRFYYSVFGEHEVFFHMPIPE
                     IMLGLSLAAMFVASFVAALQDNLKKLLAYSSVAQIGYITLGLSFDSVHGLTASLTHLF
                     NHAVTKGALFMLVGGAVAMLGNASFSRLAGLARTMPLTSFGIVIGGLGLIGVPGTAGF
                     VGKWYLILAALEKGQWLVVFAILASSLLAVVYVWRFVEQAYFREPAADTPKGEAPLAM
                     LVPSLLLLAATVWLGFDTSLTVGSASEAARMLLGVGQ"
     gene            complement(3989..4363)
                     /locus_tag="VIS73_00415"
     CDS             complement(3989..4363)
                     /locus_tag="VIS73_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HEY5761646.1"
                     /translation="MSFDSHFSYAATVFLMIAGLFIVIARGNLIKKLVGLGIFQTSVY
                     LLYVAPGKLIGGTAPILDSDFKIYSNPLPHVLILTAIVVGVATLALGLALVVRIRETY
                     GSIEEDEILLKEEAQEDSGWLQ"
     gene            complement(4360..4785)
                     /locus_tag="VIS73_00420"
     CDS             complement(4360..4785)
                     /locus_tag="VIS73_00420"
                     /inference="COORDINATES: protein motif:HMM:NF009162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit B"
                     /protein_id="HEY5761647.1"
                     /translation="MTGDLILRVVAKLLIPFVILFALYVQFHGDFGPGGGFQAGVIAA
                     AAVIFYALIYGLKAARRVVPDWLVETMLASGVLIYLFVGFAGIVLGGNFLDYSVLGST
                     TVAGQHRGIFWVEIGVLVTVCGVMLKIFYMFAGRGRKDS"
     gene            complement(4782..5294)
                     /locus_tag="VIS73_00425"
     CDS             complement(4782..5294)
                     /locus_tag="VIS73_00425"
                     /inference="COORDINATES: protein motif:HMM:NF009159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HEY5761648.1"
                     /translation="MMIVTVVAIIRQRNLFAVVVFSGIYSFLMATVLVALDAVDVAMT
                     EAAVGAGVSTVLFLGALYLGRSEEAKPTHGPWLPLLISLAAGFLVVYGTLDLPAFSDP
                     TAPIHTHVAPQYLKELARETDVPNVVTAILASFRGFDTLGETVVVFTAGAGVIALLRR
                     RRRDDQERDT"
     gene            complement(5315..5647)
                     /gene="mnhG"
                     /locus_tag="VIS73_00430"
     CDS             complement(5315..5647)
                     /gene="mnhG"
                     /locus_tag="VIS73_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HEY5761649.1"
                     /translation="MSLAIDGLSWVLLGLGSLFCLIGGVGVLRMPDFYTRMHAASVTD
                     TLGAGFILAGLLLQAGVTLIAAKLLMVGLLIFMTSPAAAHALAKAALARGLQPKLATP
                     EEDESSTR"
     gene            complement(5644..5922)
                     /locus_tag="VIS73_00435"
     CDS             complement(5644..5922)
                     /locus_tag="VIS73_00435"
                     /inference="COORDINATES: protein motif:HMM:NF015993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HEY5761650.1"
                     /translation="MFAAATIALLVSLGLALARAWLGPTVFDRLQAANSIGTIAMLLL
                     AVLGFLAGRPEFLDLALVYGLLNVVGTVAVLKYFRTGDLGRADEGGHQ"
     gene            complement(5922..6401)
                     /locus_tag="VIS73_00440"
     CDS             complement(5922..6401)
                     /locus_tag="VIS73_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEY5761651.1"
                     /translation="MSLGISAFIALLAFWLLLSGLFTPFLIAAGVGCALAVVLFSRRM
                     AVIDHEGLPTHLRFHEIALYWIWLVGEIIKSGLTVSRIILDPKLPISPTLTSFKPLQK
                     TSVGLVIHANSITLTPGTLTVRVAPDRILVHALTRSGAADAAGGSEMDRRVAALEKH"
     gene            complement(6548..>7174)
                     /locus_tag="VIS73_00445"
     CDS             complement(6548..>7174)
                     /locus_tag="VIS73_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEY5761652.1"
                     /translation="PSIFRRLYDRVFPKSADFFGMLAEQAQLVSRTASLLVEYMESGD
                     PEIGKLIKRDEHEADTVKVRNIHTLNEAFSTPIDREDIYRAIMDLDEIINYAKSTVNE
                     MDALGVKPGKFDLEIAMHIKIGVDALVNGFGRLKTEPALAAGDADLARKAERKVEKAY
                     RRALADLFQGDDYISMFKHREIYRHLSNSADRMAHCANTLHDIVVKMC"
BASE COUNT         1489 a         2172 c         2443 g         1070 t
ORIGIN      
        1 gagcagcgcg tagacgatgc cgtagaagat catgaagatg cccagccaga tcaggatttc
       61 ggtgccggga aagccgcgca gcaggacgta gaccgccgtt ttagtagtga acgccgagag
      121 gaataccgtg ccgctccacg aggcctccgg ataggcgtcg ggcagccagg cagagatcat
      181 cggcgcgccg gcgttgataa ggaagccgat caggatcagt acgttggccg ccgagtccgc
      241 ttgcatcacg ccaaacgccg tcgatccggt cgcgacgata tggccggtga tgccggcaaa
      301 aagcacgacc ccgccaaaca ggtggatcgc cagataacgc ttgctggccc ggaaggcgcc
      361 ttcggtcgct gcgctccaca gcaccagcgt cgagccgacc gccatcaatt cccagaagat
      421 gaagaccgtg accagatcgc cggcgaaggc gacgccgatc gcggagccgg cgtaaacgaa
      481 ggcagccggc acttcgatcc ggctcttctg cccgagcgcg aacaggccac cgccgaaagc
      541 catcagcgcg aagatcgtcg cgaacaggcg ggtcagcttg tcggcgacca gcggctgcag
      601 ctcgaggccg agccagctca gctgccaatg gactccgtcg ccaagctgcc aggtcagccc
      661 cagcgagacg agcggtgccg caaggatgag cacggcgcgg gcatgcccgg acagcaaggg
      721 gagaacaaag gcggccgcga tcagccagag ccccggatgc atcagccact cactcatcgc
      781 gggccggccc cccgtccgtc tcgtcgtaat aggtatcggg ccgtttgagc agcaagccca
      841 gccccttggc gccgacaatc atcgcggcac aggccaggaa gccgaaaatc gcgtggaagc
      901 caaaccagga ttcgacctcg aaatgcgggt gcagggcgac gaccagttcg gcgaccacgg
      961 tcagggccag tacgccgaga aagccccacc acaacttgcg gacgttttcg ggatgatcga
     1021 gccaatgcgt cttttccggg gtctgcatca gatgcctccg agtgccgcgc cggcaagcgc
     1081 gagcggtacg tcggggaaga agaacagcgc gatcgtcccc agtgcggtca gcgtcagcgc
     1141 aacgaccatc ggcagcggcg attcgccgtg cggatgcgct tcgtcgtttg cggaaagcgg
     1201 gcggaagaag gcacggtaga tgatcggcat gaaatacgcg gcgttcagca gcgtgctcgc
     1261 ggcgatgacc gtcagggcca tccagtgcgc ctgtgcgatc gcgccggaaa ccaggaacca
     1321 cttgctcacg aagccggcgg ctggcggcag accaatcatc gacagcgcgc cgatggcgaa
     1381 tgccgccatc gtccacggca tgcgccggcc gattccatcg agttggctga cctcggtctt
     1441 gtgtgccgcg gtgtagatcg atccggcggc gaagaacagt gtgatcttgc ccagcgcatg
     1501 cgcggcgata tggagaattg cggcgaccat cgacaagggt gccagcagcg ccgcggccag
     1561 gacgacatac gaaagctggc tgactgtcga ataggcaagg cggcgtttca ggttgtcggc
     1621 gttaagcgcc gtgatcgagg cggcgacgat ggtgaatccg gaaaccgcga cgagccagtc
     1681 ggtccccgcc agttgcagct ggtcgacgcc gaagacgtag atcatcacct tgacgacact
     1741 gaagacgccg gccttgacga cggcaaccgc gtgcagcaag gccgacaccg gcgtcggcgc
     1801 gaccatcgcc gagggcagcc aacggtggaa gggcatcagc gctgccttgc cgatgccgaa
     1861 catgtacagc gcgaggaggc cgcccagcgc cagcgagccg gcaccctcgg gcaggatgcc
     1921 gccgggcgtg aactcggtgg tgccggcgag gtaccaggtg aagacgatcg ccggcaacag
     1981 aaagaggatc gaggtcgaca gcagcacgcc gagatagacc cgcgcgccgc gcttggcgtg
     2041 gtcggttccg tggtgcgtga ccagcgggta ggtcaccagc gtcagcatct cgtagaagac
     2101 gaacagcgtg aacgcgttgc cggcgaaggc gatgcccatc gccgcggcga tcgccagggc
     2161 aaagcagacg tagaagcgcg tctggtgctt ctcgtcgttg ccgcgcaggt agccgatcga
     2221 ataaatcgaa ttgatgatcc acaagcctga ggcaatcagc gcgaaaagca tgccgagcgg
     2281 ctcgagccgg aaggacagcg ccaggcccgg cagtatctcg acgatggaaa gctcgggccg
     2341 atcgcccgcc agtacaccgg gaagcaggct ggcgaccgtt gcgaagagca gcacggcagt
     2401 caccagcgtc accgtttcgc gcaggttcgg tgcacgatgg caggcaacga tgccgagcgc
     2461 gcctgcgact gggatgacga tgccgccggc cagcgcgagg gtggcactca ttggccgact
     2521 cccagcagca tgcgcgccgc ctcgcttgcc gaaccgacgg tcagcgaagt atcgaagccc
     2581 agccagaccg ttgcggcgag cagcagcagt gacggaacca gcatcgccag cggcgcctcg
     2641 cccttcggtg tgtcggcggc gggctcgcgg aagtacgctt gctcaacgaa gcgccagacg
     2701 tagacgactg ccagcaggga actggcgagg atcgcgaaga cgaccagcca ctgccccttc
     2761 tccagggccg ccaggatcag gtaccacttg ccgacgaagc ctgcggtgcc gggaaccccg
     2821 atcaggccga ggccgccgat gacgatgccg aacgaggtca gcggcatcgt tcgcgccaat
     2881 ccggcgaggc gggagaacga cgcgttgccg agcattgcca ccgcgccgcc aaccaacatg
     2941 aacagcgcgc ccttggtcac ggcatgattg aacaggtgcg tcagcgaggc ggtgaggcca
     3001 tgcaccgagt cgaaggagag gccgagcgtg atgtagccga tctgcgcgac gctggaatag
     3061 gcgaggagct tcttcaagtt gtcctgcagg gcggctacga aggaggcaac gaacatcgcc
     3121 gcgagcgaca gcccgagcat gatctcgggg atcggcatat ggaagaacac ctcgtgctcg
     3181 ccaaacaccg agtaatagaa gcggatcagc agatagactg aaaccttggt tgccgtgccg
     3241 gcgaggaatg ccgaaaccgc cgacggcgcg taggtgtaag cgttgggcag ccactgatgc
     3301 agcggaaaga gcgccagctt gagggaaatg ccgaccgtca gcatcgccag cgctgccagc
     3361 accggccggt ggctggtcat ttcctggagg cgcacgctca tgtcggcgag attcagcgtg
     3421 ccggtcttca ggtagagaag gccgacgccg atgacgatga aggtggcgcc tatcgtgccc
     3481 atgatcaggt actggaacga ggccagcagc gcccgtcgat cgcggccaag cgcgatcagc
     3541 acataggttg acagcgagga gacctcgagg aacacaaaga cgttgaacgc gtcgccggtg
     3601 atcgtgatgc cgaggaggcc ggcgaagcac aaggccatca tcgcgtagaa caggtaatgc
     3661 tgctccgggc ggacctcgcg ggcgacgctc gcgcggcagt acgggagcgt gatcgcggcc
     3721 atgccggaga ccagcaccag gatgaaggca gacagccggt cgacgcggta ctcgataccc
     3781 cagggcgggc gccagttgcc catcgcgtag gaaatcaccg agccatcggc gacttgcagc
     3841 cagagcagga cggcgatgac gagtgccgcc cacgaggcgg cgagcaccac aatgaaggcg
     3901 aatccggcgc ggcggccgag gactgcaagc gtggcggcca gcagcggcaa ggcaacctgc
     3961 agggcaggca aatgggtttc cagattcatc actgcagcca gcccgaatct tcctgtgctt
     4021 cttcctttaa caggatttcg tcttcttcaa tgctgccgta ggtttcccgg atacgtacga
     4081 ccagcgccag gccgagcgcg agcgtggcga cgccgacgac gatggctgtc aggatcagca
     4141 cgtgcggaag cgggttcgag taaatcttga agtcgctgtc aaggatgggc gcggtaccgc
     4201 cgatcagctt tcccggggca acgtagagca gatagacgga ggtctggaag atgccgaggc
     4261 caaccagttt cttgatcagg ttgccgcggg caatgacgat aaacagcccg gcgatcatca
     4321 ggaacaccgt ggccgcgtag ctgaagtggc tgtcgaagct caagagtcct tccttccccg
     4381 gccggcgaac atgtagaaga ttttcagcat cacgccgcag acggtgacca gaacgccgat
     4441 ctcgacccag aagatgccgc ggtgctggcc ggcaaccgtc gttgaaccca gcaccgagta
     4501 gtcgaggaaa ttgccgccga gcacgatgcc ggcgaagccg acgaacaggt agatcagcac
     4561 gccgctggct agcatcgttt cgaccagcca gtccggaacc acccgccggg ccgccttgag
     4621 accgtaaatc agcgcataga agatgactgc ggcggcggcg atgacgcccg cctggaagcc
     4681 gccgccgggg ccgaagtcgc cgtgaaactg cacgtagagc gcgaacagaa tcacaaaggg
     4741 gatcagcagc ttggcgacga cgcgcaggat caggtcgccg gtcatgtgtc gcgctcctgg
     4801 tcatcgcgcc ggcggcgccg caggagggcg atgacaccgg cgccggcggt gaataccacg
     4861 acggtctcgc cgagcgtgtc gaagccgcga aagctcgcca ggatcgccgt gacgacgttg
     4921 ggcacgtcgg tttcgcgcgc tagttccttg aggtactgcg gcgcgacatg ggtgtggatg
     4981 ggtgcggtcg gatccgagaa tgccggaaga tccagcgtgc cgtagacgac gaggaagccc
     5041 gcggctaggg agatcagcag tggcagccag gggccgtggg tcggctttgc ttcttcgctt
     5101 cgcccgaggt agagcgctcc gaggaagagc acggtcgaaa cgccggcacc aacggcggct
     5161 tcggtcatcg ccacgtccac ggcgtcgagg gcgaccagca cggtcgccat caggaagctg
     5221 tagatgccgc tgaagacgac aaccgcgaaa agatttcgct gtcggatgat cgcgaccaca
     5281 gtcacgatca tcatcaccag cagcgtgtcg atgatcagcg agtcgatgat tcgtcctcct
     5341 cgggagtcgc caacttgggc tgaaggccgc gggccagcgc agccttggcc agcgcatgcg
     5401 ccgctgccgg actggtcatg aaaatcagca ggccgaccat caggagtttc gcggcaatca
     5461 gcgtcacacc ggcctgcagc aggagcccgg caaggatgaa cccggcgccg agcgtgtcgg
     5521 tgacgctggc ggcgtgcatg cgcgtgtaga agtccggcat gcgcagcacg ccgacgccac
     5581 cgatcaggca gaacaggctg ccgaggccga gcagcaccca tgacagtccg tcgatcgcca
     5641 ggctcattga tgcccgcctt cgtcggcgcg gccgaggtcg ccggtccgaa agtacttgag
     5701 gaccgcgacg gtgccgacga cattgagaag cccgtagacg agggcgaggt cgaggaactc
     5761 cgggcgaccg gcgaggaacc cgagcacggc aagcagcaac atcgcgatcg ttccgatcga
     5821 attggcggcc tgcagccggt cgaacaccgt cggtccgagc caggcgcgcg ccagcgcgag
     5881 gccgagcgaa acgagcagtg cgatcgtggc ggcggcgaac atcagtgctt ctccagtgct
     5941 gcaacccgac ggtccatttc gctgccgcct gcggcatcgg ccgcgccgct tcgcgtcaag
     6001 gcatggacga ggatgcgatc gggggcaacc cgaaccgtca gtgtgcccgg ggtcagcgtg
     6061 attgaattcg catggatgac cagaccgacg ctggtcttct gcaagggctt gaacgaggtg
     6121 agcgtcgggc tgatcggcag cttggggtcg aggatgatgc ggctgaccgt gaggccggac
     6181 ttgatgatct cgccgaccag ccagatccag tacagggcta tctcatggaa gcggaggtgg
     6241 gtgggcagtc cttcatggtc gataacggcc atgcgccgcg agaacaggac gacggcgagc
     6301 gcgcagccga cgccggcggc aatcaggaag ggggtgaaca gtcccgaaag gagcagccag
     6361 aatgccagca gggcgatgaa ggcgctgatt ccaaggctca aggtattgcc tcgatcggtg
     6421 ggggcgtcgt tagccgatgt gtcgggggaa gcgctctgtg atctcggtca caggaggcgg
     6481 cggagtttaa catgggtcga ccgtggcgca aggccgcaag tggcttcacg tgcggcgcgc
     6541 agaccgatca gcacatcttg accacgatgt cgtgcagggt gttcgcgcaa tgcgccatcc
     6601 ggtcggccga gttcgagaga tgccgataga tttcacggtg cttgaacatg ctgatgtaat
     6661 cgtcgccctg gaagaggtcg gcgagtgccc gccggtaggc tttctcgacc ttgcgctcgg
     6721 ccttgcgggc gaggtctgcg tcgccggccg ccagcgccgg ttcggtcttt agccgcccga
     6781 agccattgac gagcgcatcg acgccgatct taatgtgcat cgcgatctcg aggtcgaact
     6841 tgccgggctt gacgcccagc gcgtccattt cgtttacggt gctcttggcg tagttgatga
     6901 tctcgtcgag atccatgatc gcccggtaga tgtcctcgcg atcgatcggc gtcgagaagg
     6961 cttcgttcag cgtgtggatg ttgcgcacct tgaccgtgtc cgcttcgtgc tcgtcgcgct
     7021 tgatcagctt gccgatctcg gggtcgcctg actccatgta ctcaaccagc agactggccg
     7081 tgcgcgacac gagctgcgct tgctcggcga gcatgccgaa gaagtcggcc gatttcggaa
     7141 agacccggtc gtagaggcgg cggaagatcg aggg
//
LOCUS       DATDAA010000012         3697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_384831,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000012 DATDAA010000000
VERSION     DATDAA010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384831"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VIS73_00450"
     CDS             <1..893
                     /locus_tag="VIS73_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY5761653.1"
                     /translation="RRRGIGVELIDAEAGYFRLSFGGRQITCRESLTELTTAVAMSRC
                     DDKRITHRVLGTAGLRVPEQIEPRDPIETEAFIARHQRVVVKPARGEQGYGVRVDLRS
                     PMTVHAAVAALRDGGDAPLIEQMVAGEDVRIIVIDYQFVAAATRRPAEVVGDGRHTLA
                     ELIEKQSRRRQAATGGESRIPLDAETRACIELQGVTLDDVLPEGRTMRVRKTANLHTG
                     GTIHDVTERIRPELREVAVRAARALGIPVVGFDFMVPDLDGTDYVIIEANERPGLANH
                     EPQPTAQRFIDLLFPQTRTQ"
     gene            902..2035
                     /locus_tag="VIS73_00455"
     CDS             902..2035
                     /locus_tag="VIS73_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant NAGGN system M42 family
                     peptidase"
                     /protein_id="HEY5761654.1"
                     /translation="MTTIQTPDIDTNYLRTVLQRLLDVPSPSGMTDAVTHVVCEELAK
                     LKIPFSLTRRGAIRADLEGGKHSPDRAIVSHLDTLGAMVKQFKDSGRLEIVPIGSWSA
                     RFAEGARVTIYCDGGKQFRGTILPCKASGHTFNEEVDTQPSAWHNLELRIDERFNVAA
                     DPAAAGINIGDHISIDPQPEFLDNGFINSRHLDDKAGVAAILAMAEAVRRQALVLPVD
                     CHLLFTISEEVGVGASHVLHGDVAELISVDNGTIAAGQNTSEFGVTVSMQDSSGPFDW
                     HLTRHLLDLCAAHRIESSRDVFRFYRSDAAAAVEAGNDIRTALVCFALDGSHGYERTH
                     IDSLTALARLLGCYIQSRPLFTRDRAALGPLEDFPAADNQLHC"
     gene            2383..3693
                     /gene="aceA"
                     /locus_tag="VIS73_00460"
     CDS             2383..3693
                     /gene="aceA"
                     /locus_tag="VIS73_00460"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEY5761655.1"
                     /translation="MTDRKQQIADLEKDWASNPRWKNVKRGYAAEDVVRLRGSMPIEH
                     TLAKRGAEKLWAKVNGGAKKGYVNAFGAITAGQAMQQAKAGLEAVYLSGWQVAADGNT
                     SETMYPDQSLYAYDSVPTMVRRINNTFKRADEIQWGRGINPGDKEFVDYFLPIVADAE
                     AGFGGVLNAFELMKNMITAGAAGVHFEDQLAAVKKCGHMGGKVLVPTQEACEKLIAAR
                     FAADVMGVPTLILARTDAEAANLITSDHDANDKPFLTGERTQEGFYRVKNGLEQAISR
                     GVAYAPYADLVWCETGTPDLGFAREFAQAVQKACPGKLLSYNCSPSFNWKKNLDDKTI
                     AKFQDELSALGYKYQFITLAGIHINWYNTFQFAHAYARGEGMKHYTEMVQEPEFAARD
                     KGYTFVSHQQEVGAGYFDDVTTVIQGGSSSVKALTGSTEEEQFH"
BASE COUNT          724 a         1293 c         1094 g          586 t
ORIGIN      
        1 cgcgccggcg cggcatcggc gtcgagctga tcgacgccga ggccgggtac ttccggcttt
       61 ccttcggcgg ccggcagatc acctgccgtg aatcgctgac cgagctgacc acggcagtgg
      121 cgatgagccg ctgcgacgac aagcgcatca cccatcgcgt actcggtact gccggcctgc
      181 gcgtccccga gcagatcgag ccgcgcgatc ctatcgaaac cgaggccttc attgcccgcc
      241 accagcgggt cgtggtcaag ccggcgcgtg gcgaacaggg ttacggcgtg cgcgtcgacc
      301 tgcgctcgcc gatgaccgta cacgcggcag tcgccgcgct gcgggacggc ggcgacgcgc
      361 cgctgatcga gcagatggtc gccggcgagg acgtacgtat catcgtcatc gactaccagt
      421 tcgttgccgc ggcgacgcgc cgcccggccg aagtcgtcgg cgatggccgg cacacgcttg
      481 ccgaactgat cgaaaagcag agtcgccgcc ggcaggcggc aaccggcggc gagagccgta
      541 taccgctcga tgccgagacg cgcgcctgca tcgagctgca gggcgtcacg ctcgatgacg
      601 tcttgcccga aggccgaacg atgcgtgtgc gcaagaccgc caatctgcac accggcggca
      661 ccatccatga tgtcaccgag cgcattcggc ccgaactgcg cgaggtggcg gtacgcgctg
      721 cacgcgcgct cggcatcccg gtggtcggtt tcgacttcat ggtcccagac ctcgatggca
      781 ccgattacgt gattatcgaa gccaacgagc gcccgggact tgccaaccac gagccgcagc
      841 cgacggcgca acgtttcatc gatctgctgt ttccgcagac cagaacccaa taaggaaaac
      901 catgaccacg atccagacgc ccgacatcga caccaactat ctgcgcaccg tgttgcagcg
      961 gctgctcgac gtgcccagcc cgtcgggcat gaccgacgcg gtgacgcacg tggtctgcga
     1021 agaactggcg aaactgaaga tcccgttcag cctgacccgt cgcggcgcga tccgcgccga
     1081 cctcgaaggc ggaaagcaca gccccgaccg cgcgatcgtc tcccacctcg atacgctggg
     1141 cgcgatggtc aagcagttca aggacagcgg gcgtctcgaa attgtcccga tcggctcctg
     1201 gtcggcacgt ttcgccgaag gcgcccgcgt caccatctac tgcgatggcg gcaagcagtt
     1261 tcgcggcacc atcctgccct gcaaggcctc gggccatacc ttcaacgagg aggtcgacac
     1321 ccagccctcg gcctggcaca acctcgaact gcgcatcgac gagcgcttca atgtcgccgc
     1381 cgatcccgct gccgccggca taaacatcgg cgaccacatt tctatcgacc cgcaacccga
     1441 gttcctcgac aacggcttta tcaactcgcg tcacctcgac gacaaggccg gcgtcgcggc
     1501 gatcctggcg atggccgagg ccgtgcgccg tcaagcgctg gtgctgccgg tcgactgtca
     1561 tctgctgttc acgatttcgg aggaagtcgg cgtcggcgcc tcgcacgtgc tgcacggcga
     1621 cgttgccgag ctgatctcgg tcgacaacgg cacaatcgcc gccgggcaga acacctcgga
     1681 attcggcgtt acggtgtcga tgcaggattc gagcggcccc ttcgactggc acctgacgcg
     1741 ccacctgctc gacctctgcg ccgcgcatcg catcgaatcc tcgcgcgacg tcttccgttt
     1801 ctaccgcagc gacgccgctg cggcggtcga ggccggcaac gacatccgca ccgcgctggt
     1861 ctgcttcgca ctcgacggct cccacggcta cgagcgcacc cacatcgact cgctgaccgc
     1921 gctggcgcgc ctgctcggct gctacatcca gagccgtccg ctgtttaccc gcgaccgtgc
     1981 ggcgctcgga ccgctcgagg atttcccggc cgccgacaac cagctgcact gctgacccgg
     2041 cgcgtgcccg acggctgcgg gcgccgatgg tgcggcggcc gcagccgtcg cgcagaggtc
     2101 tgaccatttg acgacaagag ggccgcccgc gcaggattcg ccgggcggct atttcatttt
     2161 ggcgcagccc gttccacatt gcgggatgga gcatgcaaca cactgttttt cattggaaac
     2221 aaaatatcag caaatgccac tttaggcctt gttcgcagca gagcgcctat ctatactctg
     2281 acccacaaac ttatgtctta tataagacat aacagttgac tggttcgggg ccaaccccgg
     2341 cgaccaacga attccgcaat caacgacaac gaggcaatca acatgaccga ccgcaaacag
     2401 caaatcgccg acctggaaaa agactgggcc tccaaccccc gctggaagaa cgtcaagcgc
     2461 ggctacgccg ccgaggacgt cgtccgcctg cgcggctcaa tgccgattga gcacacgctc
     2521 gccaagcgcg gcgccgaaaa gctctgggcc aaggtcaacg gcggcgccaa gaagggctac
     2581 gtcaatgcct ttggcgcgat caccgccggc caggccatgc agcaggccaa ggccggccta
     2641 gaagccgtct atctgtcggg ctggcaggtc gccgccgacg gcaatacctc cgaaacgatg
     2701 taccccgacc agtcgctata cgcttacgac tcggtgccga cgatggttcg ccgcatcaat
     2761 aacaccttca agcgcgccga tgaaatccag tggggccgtg gcatcaatcc cggcgacaag
     2821 gaattcgtcg actacttcct gccgatcgtc gccgacgccg aggccggctt tggcggtgtg
     2881 ctcaacgcct tcgaactgat gaagaacatg atcaccgccg gcgcagccgg tgtgcacttc
     2941 gaagaccagc tcgccgccgt caagaagtgc ggccacatgg gtggcaaggt tctggtgccg
     3001 acccaggaag cctgcgagaa gctgatcgcc gcgcgcttcg ccgccgacgt catgggcgtg
     3061 ccgacgctga tccttgcccg caccgacgcc gaagccgcca acctgatcac ctccgatcac
     3121 gacgccaacg acaagccctt cctcaccggc gagcgcacgc aggaaggctt ctaccgcgtc
     3181 aagaacggcc tcgagcaggc gatcagccgc ggcgtcgcct acgcgcccta cgccgaccta
     3241 gtgtggtgcg agaccggcac gcccgacctc gggttcgccc gcgaattcgc gcaggcggtg
     3301 cagaaggcct gtccgggcaa gctgctctcg tacaactgct cgccctcgtt caactggaag
     3361 aagaatctcg acgacaagac aatcgccaag ttccaggacg aactctcggc cctcggctac
     3421 aagtaccagt tcatcacgct ggccggcatc cacatcaact ggtacaacac cttccagttc
     3481 gcccacgcat atgcccgcgg cgaaggcatg aagcactaca ccgaaatggt gcaggagccg
     3541 gaattcgccg cccgcgacaa aggctacacc tttgtctcgc accagcagga agtcggcgcc
     3601 ggctacttcg acgacgtcac caccgtcatc cagggcggct cgtcctcggt caaggccctt
     3661 accggctcga ccgaagaaga gcagttccac taagaac
//
LOCUS       DATDAA010000013         2070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_70425,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000013 DATDAA010000000
VERSION     DATDAA010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70425"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VIS73_00465"
     CDS             <1..267
                     /locus_tag="VIS73_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEY5761656.1"
                     /translation="PTYVVHDSVHSCVTNMPGAVPRTSTFALTNATLPFVKNLANLGW
                     RDALRRDPHLANGLNVHAGHVNHEAVAHDLGYEYLSADDALNAA"
     gene            complement(340..1107)
                     /locus_tag="VIS73_00470"
     CDS             complement(340..1107)
                     /locus_tag="VIS73_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY5761657.1"
                     /translation="MSEFELSIIFPAFIACLLVLSTHVPLGVEVLRRGIIFIDLAIAQ
                     VAGLGVIAADTMGWEARGWMVQFAAVSAALVAAGILHWTDKRWPEIQEAIIGASFILA
                     ATAGILILAGNPHGGEHLKELLVGQVLWVNIDQLLPVAVISVLVVAAWFWKREHLRGL
                     GFYALFAFAVTASVQLVGVYLVFASLIIPALATRHIEHRWWRVSSGYGVGVVGCLAGL
                     VLSVGADLPAGAIVVWSLAITALVFAWLVWPLFRQSK"
     gene            complement(1104..1997)
                     /locus_tag="VIS73_00475"
     CDS             complement(1104..1997)
                     /locus_tag="VIS73_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEY5761658.1"
                     /translation="MIRVITVLIALWLPVSANALNVFSCEPEWAALVEELAGDEADVT
                     VATTAFQDPHMLQARPSLIAGARNADLLICTGADLEIGWLPLLLRRAGNPAIQPGSPG
                     HFMAADHVRRIEIPASVDRSQGDVHPQGNPHIHLHPRNIERVAEALADRLVQLDPANA
                     ESYRASAEDFLQRWYDARDRWEERAAPLAGMRLVSHHRSFSYLADWLGLEIVATLEPK
                     PGIPASGAHLSRLLEQLTPAPPAGIIRAPFENEKPAHWLSDRLNIPMIEMAFTVGGSD
                     DVTDLFALYEQTIAALEEYRE"
BASE COUNT          387 a          691 c          655 g          337 t
ORIGIN      
        1 ccgacttacg tcgttcacga tagcgtgcac tcctgcgtaa ccaacatgcc gggtgcggtg
       61 ccgcgcacga gcaccttcgc gttaaccaac gccacgctgc cgttcgtgaa gaacctggcg
      121 aatctcggct ggcgcgacgc cttgcgccgc gacccgcacc tcgcgaacgg actgaacgtt
      181 catgcagggc atgtcaatca cgaagcggtt gcccacgatc tcggttacga gtatctctcc
      241 gccgacgatg ccctcaacgc tgcctgaccc cagagctttg agctctattc cccctgcagg
      301 ttaggcacat cccaggccgg gcctcgtgcc cggcctgttt tatttcgatt gccggaacag
      361 gggccagacc agccaggcga agacgagcgc cgtgatcgcc aggctccaca ccacgatcgc
      421 gccggcaggc aggtcggcac ccaccgacaa cacgagccct gcgaggcaac cgacgacgcc
      481 gacgccgtag ccgctcgata cccgccacca gcgatgttcg atgtgacgcg tcgccagcgc
      541 cgggatgatg agcgaagcga acacgaggta aacgccgacg agctgcaccg aggcggtaac
      601 ggcaaacgcg aacagcgcat agaacccgag gccgcgcagg tgctctcgct tccagaacca
      661 ggcggcgacc accagcacgc tgatcacggc gaccggtaac aactggtcga tatttaccca
      721 cagcacctgg ccaacgagga gttccttcag gtgttcgccg ccgtgcggat tgccggccag
      781 gatcaggatg ccggccgttg ccgcgagtat gaacgacgcc ccgatgatcg cttcctgaat
      841 ttccggccag cgcttgtccg tccagtgcag gatgccggca gcgaccagtg cggccgacac
      901 tgcggcgaac tgcaccatcc agccgcgtgc ttcccagccc atcgtgtccg cggcaatgac
      961 gccgagaccc gcgacctggg cgatcgcgag atcgatgaag atgatgccgc ggcgcaagac
     1021 ctctacgccg agcgggacgt gggtgctgag caccagcagg catgcgatga acgcgggaaa
     1081 gatgatgctc agctcgaact cgctcactcg cgatattcct ccagcgccgc gatggtctgc
     1141 tcgtagagcg cgaacaggtc cgttacgtcg tcgctaccgc caacggtaaa cgccatctcg
     1201 atcatcggaa tattaagcct gtccgacaac cagtgcgcgg gtttctcgtt ttcgaagggc
     1261 gcgcggatga tgccggcagg cggcgcaggt gtgagctgtt ccagaaggcg cgacaggtgt
     1321 gcaccgctcg cggggatgcc cggcttgggc tcgagcgtgg caacaatctc gaggcccagc
     1381 cagtctgcga ggtagctgaa gctgcgatgg tgcgaaacga gacgcatgcc ggcgagcggt
     1441 gccgctcgtt cttcccagcg gtctcgagcg tcataccagc gctgcagaaa gtcctcggcg
     1501 ctcgcacgat agctttcagc gttggccgga tcgagctgca ccagccggtc ggctaacgcc
     1561 tcggcaacgc gctcgatgtt gcgcgggtgc aggtgaatat gcggattgcc ctgcgggtgc
     1621 acgtcgccct gcgaacggtc cacgctcgcc gggatctcga tgcggcgcac gtgatccgcc
     1681 gccatgaaat ggccggggct gccgggctgt atcgccgggt tgcccgcacg cctgagcagc
     1741 agcggcaacc agccgatctc gaggtcggcc ccggtgcaga tcagcagatc cgcgttgcgg
     1801 gcacccgcga tcaggctcgg tcgcgcctgc agcatgtgcg ggtcctggaa cgctgtcgtc
     1861 gccaccgtga cgtcggcctc gtcgccggcg agttcctcga cgagcgcggc ccactcgggc
     1921 tcgcagctga acacgttgag cgcgtttgcc gacaccggca gccaaagcgc gattagtacg
     1981 gtaataaccc gaatcatggc cggcccccta gaactggtgc gcgccgtgcg cgccgatgct
     2041 catgatgtac tgcaggtacc agacgctgtc
//
LOCUS       DATDAA010000014        18004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_350206,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000014 DATDAA010000000
VERSION     DATDAA010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 18004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18004
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350206"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VIS73_00480"
     CDS             <1..454
                     /locus_tag="VIS73_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761659.1"
                     /translation="IPDAEAALTMLREIDATQPELPRLESLLRLLQINAAMREAYDSA
                     AQAAAWESLETLAVEGPLADLRPEGDFALSELRSSQLVRAGGALAADRLGECREALLQ
                     ARVLARVLGDVAAPPEENRFVDRLQRAADAAAARRSRPSSARLPRSAA"
     gene            complement(830..1588)
                     /gene="zapD"
                     /locus_tag="VIS73_00485"
     CDS             complement(830..1588)
                     /gene="zapD"
                     /locus_tag="VIS73_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEY5761660.1"
                     /translation="MIVFEYPLNERIRTMLRLEDLFEKIAHFERGEGPREHHVALTTL
                     FEVLEVAWRGDLKVDLIQELDRQRQSLLAFRNNPQISEEALSGALYEIEQASTALLGL
                     AGKVGQGIRDNEWLMSIKGRAGIPGGVCEFDLPSYHLWLHRPVAVRREQFSGWLKPFV
                     PIRDGLAIVLRLLRSSGRSEEKLAAKGVFQMTPSGIAVQMIRVETEDDEGCVPEVSAN
                     KYALNIRFTCPDAGGARAKSCERDVRFRMTLCNL"
     gene            complement(1689..2291)
                     /gene="coaE"
                     /locus_tag="VIS73_00490"
     CDS             complement(1689..2291)
                     /gene="coaE"
                     /locus_tag="VIS73_00490"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289434.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY5761661.1"
                     /translation="MSVPVIGLTGGIGSGKSTVADLFAARGAGVVDTDRIAHALTSSC
                     GAAMPAIREAFGAALLADDGSLDRPAMRRLVYGEPAQRQRLEAILHPLIREQARAECA
                     VAAGDYILLVVPLLAETGALRPLMRRVLVVDCDPAVQRERVMRRSGLAAAEADAIIAA
                     QASREARLALADDVIVNDRARSELVPQVDRLHALYCRVDA"
     gene            complement(2334..3071)
                     /locus_tag="VIS73_00495"
     CDS             complement(2334..3071)
                     /locus_tag="VIS73_00495"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY5761662.1"
                     /translation="MRTVVLLLVIANLVLWGASRLGVGGSPAGEPERLAQQIGPERLK
                     IVARGEPPPATAVPAMATKPAEKPAVELACLSFGGLADDLADALVPIVTRRAPELMVA
                     RDRDQIHINGHRVQIDQLGSRAQAERKARELRDLGVNDYTIVGNGGIWVVSLGVFSNE
                     AAARSRLSALRKRGVRSAEVQAITYPDSRARVTVSGAAEAVARAGEAVRQSQPELTPE
                     TCVAPPAPDGEDESTDEARPANGKNPA"
     gene            complement(3081..4448)
                     /locus_tag="VIS73_00500"
     CDS             complement(3081..4448)
                     /locus_tag="VIS73_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEY5761663.1"
                     /translation="MSYTRIYLPAGDMPTHWYNIIADMPNPPAPPLGPDGQPVTPEKL
                     GEIFPGAILEQEMSGERWIPIPDAVREAYGLWRPAPLCRAVRLEQALGTPAKIFYKYE
                     GVSPAGSHKPNTAVPQAYYNKQAGITRITTETGAGQWGSSMAFAGQMFGLEVRVYMVK
                     VSYGQKPFRRSMMQTWGAEVLASPTNLTQAGRGILERDPENQGSLGIAISEAVEEAAS
                     RADTNYALGSVLNHVLLHQTVVGLEAKKQCEIAGVYPDVIFGPCGGGSSFGGLAFPFL
                     VDKLNGDKRAAKLRCVAVEPTSCPTLTKGAYAYDFGDASGYTPLMKMYTLGHDFMPPG
                     IHAGGLRYHGDSPLVSQLVHEKLIEALAVPQLATFEAGVAFSRAEGIIPAPESCHAIR
                     AAIDEALRCKQSGEAKTILFNLTGHGHFDMAAFDRYFAGELEDFDYPEQAVADSLAQL
                     PKVAA"
     gene            complement(4633..6051)
                     /locus_tag="VIS73_00505"
     CDS             complement(4633..6051)
                     /locus_tag="VIS73_00505"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5761664.1"
                     /translation="MHEYPRHAAFTGRVLIVGFGSIGQGFLPLLLRHVDMPAERVAVI
                     TADADGADIAARYGVSFTQCAITPENYRETLAPRIGAGDFLLNLAVNVSSLALMEFCQ
                     ARGAHYLDTCIEPWAGGYIDCGQSMSARSNYALREDALEIGRRFPGGPTAVLTHGMNP
                     GVVSHFAKQALLNLATDAGISIELPATREQWAALARRLSIKAIHIAERDTQIGPRRRS
                     RGEFVNTWSADAFLGESLQPAELGWGTHERLLPADAARHAHGSDAAIYLKRPGLLTRV
                     RSWTPLEGPYHGFLVTHAESISLADYLTVRDGARVLYRPTVHYAYHPCDDALLSLHES
                     AGHGYAPPDCSRLLMDDITDGMDELGVLLMGHARGAYWYGSRLTIGEARALSPHNNAT
                     SLQVTAGVLAGMVWAIRNPAQGIIEPEAMDHRETMEILLPYMGEMAAVYSDWTPLHGR
                     DELFPESLYTDDPWQFSNIRVS"
     gene            complement(6280..7026)
                     /locus_tag="VIS73_00510"
     CDS             complement(6280..7026)
                     /locus_tag="VIS73_00510"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY5761665.1"
                     /translation="MDSPLHAVCDLGNSRLKWGVGRLDGDTPEWVAHGALDYAALDQL
                     PGRLVAAGAAGEVLAACVAAPEREQALSAALAATGFAVRYFSAAADCCGVRNGYARPT
                     QLGVDRWAALIGSWRRLRAPGLIVCAGTATTIDALDVVGGEACFRGGVIVPGLALMTT
                     ALADGTARLPHAQGRYAVLPDNTDDAIATGIIEAQIGAIERLRRRLPPSTRCLVTGGH
                     AATLMPHIAAPAEVVDTLVLEGLLAAASVN"
     gene            complement(7029..7847)
                     /locus_tag="VIS73_00515"
     CDS             complement(7029..7847)
                     /locus_tag="VIS73_00515"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY5761666.1"
                     /translation="MTIPAEPALIDTERLVARLGPLAAHLSLSALPICDSSNAILLAS
                     AAKGAASGSVVVCDRQTSGRGRRGRSWMASPQSSLTFSLLWRFPQSARLSGLSLAIGV
                     AITRALATCGVPQARLKWPNDILLPVDGGWGKVGGVLIELSGGAAGVAAVIGVGLNLE
                     VPDGLEGLDFPAAGLADIALPPERHQLLAALLREMVAVLDRFAEAGFPVYAEAWSAQN
                     AFADQLVQLRQDDGTKRDGICRGVDGEGALWLETAVGRERVLAGDVSLRPKAPR"
     gene            complement(7902..9323)
                     /gene="ntrC"
                     /locus_tag="VIS73_00520"
     CDS             complement(7902..9323)
                     /gene="ntrC"
                     /locus_tag="VIS73_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEY5761667.1"
                     /translation="MKPVWIIDDDRAIRWVLEKALAREQIPFVSFASGTEALAQLDRG
                     DDVPQVVMSDVRMPGQSGLELLKTLKQRQPALPIIIMTAYSDLDSAVAAFQGGAFEYL
                     PKPFDVDQAIDLIRRAIDEGAHQNGAQDEVGETPEILGQAPAMQEVFRAIGRLAQSHA
                     TVLITGESGSGKELVARALHRHSPRKEAPFIAINTAAIPRDLLESELFGHERGAFTGA
                     QAQRRGRFEEAGGGTLFLDEIGDMPTELQTRLLRVLSDGHFYRVGGHQPIKANVRIIA
                     ATHQNLEGRVKDGLFREDLLHRLNVIRVRLPSLRERREDIPLLARHFMTRSAQTLGVE
                     SKRLSEPALRHLATLDFPGNVRQLENICHWLTVMAPGQTIEVADLPADLRDGEGGAVS
                     ASGSWVAALAAEVDRLLAEAGGHVHEQLIREFEKTLITRALASTGGRRIEAAQLLGIG
                     RNTLTRKTHELGLDGEPVRSEEA"
     gene            complement(9385..10470)
                     /gene="glnL"
                     /locus_tag="VIS73_00525"
     CDS             complement(9385..10470)
                     /gene="glnL"
                     /locus_tag="VIS73_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEY5761668.1"
                     /translation="MSPPLTPPADAFRGLDLLSSAVVVLDDELAVRYANAASENLLQT
                     SLAQLRGRPLNYLFGYTASLAAALDNARHNNWSYTGHNIEWRKGDAPSIHLDCTVTPI
                     SSAAGSLVLELRPIDQQIRASREERMLEQQQTNRELIRNLAHEIKNPLGGIRGAAQLL
                     EHELQSEALREYTQVIIDEADRLQNLMQRLLSPHRAMHPAEINVHEVLERVRSLLLAE
                     FTEGLVITRDYDTSLPDLIADREQLIQVFLNIARNAAQAVRGHGEIVLRTRAVRQVTL
                     AKRRYRLAVAIEVADNGPGIPPEIRDSIFYPLVTGRDDGTGLGLTLAQSFVQQHHGSI
                     ECQSEPGDTVFRVILPVDRRPNAARKA"
     gene            complement(10513..11040)
                     /locus_tag="VIS73_00530"
     CDS             complement(10513..11040)
                     /locus_tag="VIS73_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY5761669.1"
                     /translation="MKTVMPILFASLAALPAAAFADMYKCVDADGHVTYSNSSSKGCR
                     KIVIQPAVSAPAPVARPAPAAREGFPRVDEATQRARDDMRRGILEDELEGEARALAEA
                     REKLAEQETVIEPDERNVTQRCVPAASGGMNCSAVPGGINAGKREERLRPFRDKVAQH
                     ERNIESLNREISRLP"
     gene            complement(11156..12571)
                     /gene="glnA"
                     /locus_tag="VIS73_00535"
     CDS             complement(11156..12571)
                     /gene="glnA"
                     /locus_tag="VIS73_00535"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY5761670.1"
                     /translation="MAASVQDVMKMVKENDVKFVDFRFTDTRGKEQHVSVPVSAFDED
                     KFESGHAFDGSSIAGWKGIQASDMLLMPDPVTAYIDPFMDETTVVVSCDVVDPTDGKG
                     YDRDPRSIAKRAEAYLKSSGVGDTAYFGPEPEFFIFDSVEWSVDMSGSYCKVFSSEAA
                     WATAEKIDGGNTGHRPTVKGGYFPVPPVDSLHDIRSAMVMALEGVGVPVEVHHHEVAT
                     AGQCEIGTVFNTLVRRADWTQILKYVVHNVAHQYGKTATFMPKPIVGDNGSGMHVHQS
                     IWKGGQNLFAGNGYAGLSETALFYIGGIIKHARALNAITNPLTNSYKRLVPHYEAPVK
                     LAYSAKNRSASIRVPHVASAKARRIETRFPDPGANPYLCFAALLMAGLDGIQNKIHPG
                     DPADKNLYDLPPEEDAKIPTVCHALDQALDCLDQDREFLTRGGVFSNDMIDAYIALKM
                     DEVNKIRMTTHPVEYDMYYSC"
     gene            complement(12776..13987)
                     /locus_tag="VIS73_00540"
     CDS             complement(12776..13987)
                     /locus_tag="VIS73_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY5761671.1"
                     /translation="MVNDGGADEAGSSDSGLAAPDRMRVAARATWISAAVNAALTLGQ
                     LLVGWLAHSQSLIAHGLHSASDLLSDFLVLYANRHGHQPADADHPYGHARVETAATLL
                     LGISLFAVGIGILWDAVGKLRVVDTAVPVGQLAFWVAVITVIAKEGLYRYLIAVADRL
                     RSKMLAANALHTRADAASALVVVAGVGGSLLGWTFLDLLAAILMGGMILKLGVELAWS
                     AMTELIDTGLDTEEVAKIRAVLRETPGVRGVHELKTRRMAHRALVEAHLQVDSRISVS
                     EGHHIAESARARVLRTQPQVLDVLVHIDPEDDTEPPSGRPPARHQVRAALDELLGGMP
                     APVRVVVHYLQAGIEAEVFFAADAVSSEQLAEWQTVLGKRLEAQRLLVSVCLHQANAP
                     IRCNNGASQHQ"
     gene            14077..14529
                     /locus_tag="VIS73_00545"
     CDS             14077..14529
                     /locus_tag="VIS73_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY5761672.1"
                     /translation="MSSLQEILTTAQNRARELKLPYEGAVTPREAFELMQRAPGTHLV
                     DVRSKAELDWVGRIPGAVEIEWVSYPGMTRNPLFLDHLRRQVDSEGLVLFICRSGQRS
                     HFAAAVATEAGFADCYNVLEGFEGEKDTSAHRNTVGGWRVAGLPWIQG"
     gene            14705..15823
                     /gene="nadA"
                     /locus_tag="VIS73_00550"
     CDS             14705..15823
                     /gene="nadA"
                     /locus_tag="VIS73_00550"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY5761673.1"
                     /translation="MTPPTTTETAPLPFAPYNAMDDAACEVRIREARAKLGKRAVILC
                     HHYQRADIYRHADITGDSLKLSRLASQSDAEFIVFCGVHFMAEVADILSKPQQQAILP
                     DLAAGCSMADMANLAKVTRCWRELGEALGNPDEAITPVTYINSAADLKAFCGEHGGIV
                     CTSSNARTIAEWSFGKRPKILFFPDQHLGRWTGHQLGLPLEDMRVWDPDLPMGGLSVD
                     EIKAARILLWKGHCSVHQMFQLKHVQAFRAKYPDGQVIAHPECNFEVCAAADHVGSTE
                     YIVKTIKASAPGTRWLVGTELNLVSRLAEEEAPRGVTVQFMATTVCMCSTMQRIDPQH
                     LAWCLDNLVAGHVVNAISVPDHEAKLAQLALDRMLAIS"
     gene            15820..16245
                     /locus_tag="VIS73_00555"
     CDS             15820..16245
                     /locus_tag="VIS73_00555"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HEY5761674.1"
                     /translation="MIPDADEPSHFLNCLDDFRLGHEVLDREHEGLCLLINQLAEAVD
                     DQDDWEKAVALATELVHETSRHFDHEEALMKQAAYPEVDRHHEQHTELLSQIESLRDR
                     LATDHRLARPHQTLNFLGDWFTLHIDNSDRRFVAFLSES"
     gene            16397..17155
                     /locus_tag="VIS73_00560"
     CDS             16397..17155
                     /locus_tag="VIS73_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY5761675.1"
                     /translation="MTDTGLHVATYNIHKGFSQFNRRMVVHDLRDRLRTLSPDLLFLQ
                     EVQGEHHHHAARHADWPTTAQHEFIAEDVWANSAYGRNVVYDHGHHGNAILSRHPLAD
                     SHNQDVTDLAFERRGLLHTQMHLPGSAQPIHCVCVHLSLLGRSRRRQLELLAERLDHW
                     APGDAPLIVAGDFNDWRNRADPLFARKLGLSEAFGGESGRPVKSFPSAMPVFRLDRIY
                     VRGFSVESARVHSGRPWSRISDHAALSAVLRPVA"
     gene            17187..>18004
                     /locus_tag="VIS73_00565"
     CDS             17187..>18004
                     /locus_tag="VIS73_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY5761676.1"
                     /translation="MKAHFVRGNRLTLLAGGAAFFRELRAAIADAQHEVHLETYIYAD
                     DETGQAVTAELVAAAARGVLVRLLVDGFGSKPFAQAHMARLRDAGVQMMVYRPDISPY
                     ALRRRRLRRLHRKLAMIDRCTAFIGGINIVDEVPLGMAAPRLDFAVKIEGPLLAPIHH
                     SAHRMWEIVSWANFRRRYRLAANEVGCSAPSEPIGKATAALVLRDNVRRRHDIEDAYL
                     EAIEAARHDILIANAYFLPGRRFRRALLDASARGVTVRLLLQGHAEYRLQHLAT"
BASE COUNT         3021 a         6074 c         5984 g         2925 t
ORIGIN      
        1 cattcccgat gccgaggcag cgctgacgat gctgcgcgag atcgacgcga cgcagcccga
       61 gttgccacga ctcgaatcgc tcttgcgcct gctgcagatc aacgccgcca tgcgcgaagc
      121 ctacgattcg gcggcgcagg cggcggcctg ggaatcactc gaaacgctgg ccgtcgaggg
      181 gccgctcgcc gatttgcgcc ccgaaggcga ttttgcgctg agcgaattgc gctcttcgca
      241 gctggtccga gccggtggcg cactcgctgc cgatcgcctc ggcgagtgcc gcgaagccct
      301 gttgcaggca cgggtgctcg ctcgggtgct cggcgatgtc gccgcgccgc ccgaagagaa
      361 ccgcttcgtc gaccggctgc agcgcgccgc cgatgccgcc gccgctcggc gatcgcggcc
      421 ctcgtccgcg cgtcttcctc ggtcagcagc gtgacggcgc gggcgcggcc gaccgggctc
      481 cagcgtgccg gtgcctctac cagctcggac accagcgcga aggtcgacaa gggcaccaac
      541 ccttccatcg gtgccggcag tcgcgcggca tcgaggcgcg cgcgcagcgc cgcgaggtcg
      601 cgccgaacca gccgttcgac gaccgcgcca agctcgaccc cgtcgtcgcc ggcgagccgg
      661 gcgaggccgc tcttcggtgc gcgcagttcg gcgtagatcg cttcgagccg gccggccgcc
      721 tcggcctcgt ccagtgcgct cggcgtatag cgcggctcgc tggcgcagcc ggcgagcagg
      781 atcagcgaca cggcgaccag cagccggccg gcggtaatcg acttgatcat cacaagttgc
      841 aaagggtcat ccggaagcgc acgtcgcgct cgcaggactt ggcccgggcg ccaccggcgt
      901 ccgggcaggt aaagcggatg ttgagcgcgt acttgttagc gctgacctcg ggtacgcagc
      961 cctcgtcatc ctccgtctcg acgcggatca tctgcacggc gattcccgac ggcgtcatct
     1021 ggaacacgcc cttggccgcc agcttctcct cggagcgacc gctcgatcgc agcaagcgga
     1081 ggacgatcgc caatccgtcg cggatcggta cgaagggctt gagccagcca ctgaactgct
     1141 cgcggcgaac ggcaacgggc cggtgcagcc acaggtggta cgagggcaga tcgaactcgc
     1201 agacgccgcc gggaataccg gcgcggccct tgatgctcat cagccattca ttgtcgcgga
     1261 tcccctgacc aaccttgccg gccaatccga gcagcgcggt agaggcctgc tcgatctcgt
     1321 agagcgcgcc ggagagcgct tcttcggaaa tctgcgggtt gttgcggaag gccagcagcg
     1381 actgacgctg gcggtcaagt tcctgaatca ggtcgacctt gaggtcgccg cgccaggcga
     1441 cctcgagcac ttcgaacagc gtcgtcagtg ccacgtgatg ttcgcgcggc ccctcgccgc
     1501 gttcgaaatg cgcgatcttc tcgaacagat cctcgagccg cagcatcgtg cggatccgtt
     1561 cgttgagcgg gtattcgaag acgatcacgc cggcgccagt ctgtggtgcg aatagcgcgg
     1621 attctgagcg ctgaccgcaa aaacatcaac cggccgaagt gccggcaagc ccagccaggc
     1681 gggcgccatc acgcatcgac ccggcaatac agcgcgtgca ggcgatcgac ctgcggcacg
     1741 agttcggacc gtgctctatc attgacgatg acgtcgtcgg caagcgcgag gcgtgcttcg
     1801 cggctcgcct gtgcggcgat gatcgcgtcg gcttcggccg cggctaagcc gctgcgccgc
     1861 attacccgct cgcgctgcac ggccgggtcg caatcaacga ccagcacacg gcgcatcagc
     1921 ggccgcagcg ctccggtctc ggcgagcagc ggcacgacca gcaggatgta gtcaccggcc
     1981 gccaccgcgc attcggcacg cgcctgttcg cggatcagtg gatggaggat cgcctcgagg
     2041 cgttgccgct gcgccggttc gccatagacc agccggcgca ttgccggccg gtccagcgaa
     2101 ccgtcgtcgg caagcagtgc ggcgccgaag gcctcgcgga tggccggcat cgcggcaccg
     2161 caggaagaag tcagcgcatg ggcgatgcgg tcggtatcga caacgccggc gccgcgcgcg
     2221 gcgaacaggt cggcgaccgt ggacttgccg ctgccgatgc cgccggtcag gccgatcacc
     2281 ggcacgctca tgtcgccgct gccccaacag tcaacggcgg caccaagacg cactcacgcc
     2341 ggattcttgc cgttggccgg tctggcctcg tcggtcgatt cgtcctcgcc atcgggcgcc
     2401 ggtggcgcaa cgcaggtctc gggcgtcaac tcgggctggc tttgccgcac ggcctcgccg
     2461 gcacgcgcca ccgcctcagc ggcgccgctg acggtgaccc gcgcgcgcga atcgggatag
     2521 gtgatcgcct gtacttcggc agaacgaacg ccacgcttac gcagggccga cagccggctg
     2581 cgcgccgccg cctcgtttga aaacaccccg agtgacacga cccagatgcc cccgttgccg
     2641 acgatcgtgt aatcgttgac accgagatca cgcaactcgc gcgccttgcg ctcggcctgt
     2701 gcgcgcgaac cgagctggtc gatctggacc cggtgcccat tgatgtgaat ttggtcgcga
     2761 tcacgcgcaa ccatcagctc gggcgcccgg cgcgtgacga tcggcacgag cgcatcggca
     2821 aggtcgtcgg cgaggccgcc aaagctgagg caggcgagtt cgaccgctgg cttctcggcg
     2881 ggcttcgtcg ccatcgccgg caccgccgtt gccggcggtg gctcgccgcg ggcaactatc
     2941 ttcaggcgtt cgggaccgat ctgctgtgcc agccgctcgg gctcgccggc cggcgagccg
     3001 ccgacaccca gtcgtgatgc gccccacaac accaggttgg cgatgacgag gagcaggacg
     3061 acggtgcgca cgacagcagg tcaggccgcg actttgggca gctgcgcgag cgagtcggca
     3121 acggcctgtt ctgggtagtc aaagtcctcg agctcgccgg cgaagtagcg gtcgaaggcg
     3181 gccatgtcga aatgcccgtg accggtgagg ttgaagagaa tggtcttcgc ctcgccgctc
     3241 tgcttgcagc gcagcgcctc atcgatcgcc gcgcggatcg cgtggcagga ctccggcgcc
     3301 gggatgatgc cctcggcgcg cgaaaaggcg acgccggcct cgaaggtggc gagctgcggc
     3361 acggccagcg cctcgatcag cttctcgtgc acgagctgcg agaccagcgg cgaatcgccg
     3421 tggtagcgca gaccgccggc gtggatgcca ggcggcatga agtcgtggcc gagcgtgtac
     3481 atcttcatca gcggcgtgta gcccgaggcg tcgccgaagt cgtaggcgta ggctcccttt
     3541 gtcagcgtcg ggcacgaggt cggctcgacc gcgacgcagc gtagcttcgc cgcccgcttg
     3601 tcgccgttca acttgtcgac gaggaagggg aaggcaaggc cgccgaagct cgatccgccg
     3661 ccgcagggcc cgaagatcac gtcgggatac acgccggcga tttcgcactg tttcttcgcc
     3721 tcgagtccga ccacggtctg gtgaagcagc acgtggttga gcaccgagcc gagcgcatag
     3781 ttggtgtcgg cgcgcgaggc cgcctcttcg accgcttcgg aaatggcgat gccgagcgag
     3841 ccctggtttt cggggtcgcg ttcaaggatg ccgcggccgg cctgcgtcag gttggtcggg
     3901 ctggccagca cctcggcgcc ccaagtttgc atcattgagc ggcggaacgg cttctgtccg
     3961 tagctgacct tgaccatgta gacgcggacc tcaaggccga acatctggcc ggcgaaggcc
     4021 atcgacgagc cccactggcc ggcaccggtc tcggtggtga tgcgggtgat gccggcctgc
     4081 ttgttgtagt aggcctgcgg cactgcggtg ttcggcttgt gcgaaccggc cggcgaaact
     4141 ccttcgtact tgtagaagat cttcgccggg gtgccgagcg cctgctcgag ccggacggcg
     4201 cgacaaagcg gtgccggccg ccagaggccg taggcctcgc gcacggcgtc aggaatcgga
     4261 atccagcgtt cgccgctcat ctcctgttcg agaatggcgc cgggaaagat ctcgccgagc
     4321 ttttccggcg tcaccggctg tccatccgga cccagtggcg gtgccggcgg attgggcatg
     4381 tcggcaatga tgttgtacca gtgcgtgggc atgtcgcccg ctggcaaata gatacgcgtg
     4441 taactcatgt ctcccccgtg gttgcggcgc caggatcgct ccgcgggtag tctcccgggt
     4501 agcggcgccg attcgtggcg tgccttggaa aaccgcgatg gcgcggcgag gcccgtttca
     4561 gcgaggcgcg agtataccgc ctcgcgactc ctggccgctg tgctcccggc aacccgggcg
     4621 catgcgaccc gctcaggaca cgcggatatt cgagaactgc cagggatcgt cggtgtagag
     4681 gctctcgggg aacagctcgt cgcggccgtg tagcggcgtc cagtcggagt aaacagcggc
     4741 catttcgccc atgtagggga gcaggatttc catcgtctca cgatgatcca tcgcctcggg
     4801 ctcgatgatg ccctgcgccg ggttgcggat tgcccagacc atccctgcga gaacgccggc
     4861 ggtcacctgc agactggtcg cgttgttgtg cggggagagt gcccgcgcct cgcctatcgt
     4921 cagccgcgaa ccgtaccagt aggcgccgcg ggcgtgcccc atgagcagca cgccaagctc
     4981 gtccattccg tcggtaatgt cgtccatcaa cagacgggaa caatcgggcg gcgcatagcc
     5041 gtggccggcc gattcatgca gtgacagcaa cgcatcgtcg caggggtggt aggcgtagtg
     5101 cacggtcggc cggtatagca cacgcgctcc gtccctaacg gtcagatagt cggcgagcga
     5161 gatcgattcg gcgtgggtca ccagaaagcc gtggtacggg ccttcgagcg gggtccagct
     5221 gcgcacgcgg gtgagcaggc ccggccgctt gagatagatc gccgcgtcgc tgccgtgcgc
     5281 atgtcgcgcc gcgtcggccg gcagcaagcg ctcgtgcgtc ccccagccca actcggccgg
     5341 ctgcagcgac tcgcctagaa aggcgtcggc cgaccaggta ttgacgaact cgccgcggct
     5401 gcgccggcgc ggcccgatct gcgtatcgcg ctcggcaatg tggatggcct tgatcgacag
     5461 ccgccgggcc aacgccgccc actgctcgcg cgtcgccggc agctcgatgg aaatgcccgc
     5521 atcggtggcc aggttgagca gcgcctgctt ggcgaaatgc gagacgacgc cggggttcat
     5581 gccgtgcgtc aaaacggccg tcggcccacc ggggaaacgg cgaccgatct caagggcgtc
     5641 ctcgcgcagc gcgtagttcg agcgcgcgga catcgactgg ccgcagtcga tgtagccgcc
     5701 ggcccagggt tcaatgcagg tgtcgaggta gtgcgcgccg cgcgcttggc aaaactccat
     5761 cagcgccaga ctggagacgt tgaccgcaag attaagcagg aagtcgccgg cgccgatgcg
     5821 cggcgcgagc gtctcgcggt agttctccgg ggtaatcgcg cactgcgtga acgagacacc
     5881 gtagcgtgcc gcaatgtcag caccgtcggc gtcggcagta atcaccgcaa cgcgttctgc
     5941 gggcatgtcg acgtggcgca gcagcagtgg cagaaagccc tggccgatac taccgaaacc
     6001 gacgatcaac acgcgcccgg taaaggccgc gtgacgcggg tactcgtgca tggtgcacgc
     6061 ctcctctgcg ggttccgacg gcgcaatgac cgccgggcct gagccggcat ggattctctg
     6121 ggcgttctcg ccgtttccgg gaaaagcctc gggctctcgt cccgtatcct ctcggctcgc
     6181 gtacgaaaac ctgtgccatc gtcgagacgc ccgctggtag cagccggcag acgacacgcg
     6241 aggcgcgcat gctaccacca agcgcggcgc gcggcctggt cagttgacgc ttgctgcggc
     6301 gagcaggcct tcgagcacaa gggtatcgac gacctcggcc ggcgccgcaa tatggggcat
     6361 cagggttgca gcgtgcccgc cggtgaccag gcagcgcgtg cttgggggca gccggcgacg
     6421 caagcgttcg atggcaccga tttgggcctc gatgatgccg gtggcgatcg cgtcgtcagt
     6481 gttgtcaggc agcacggcat agcggccctg cgcgtgtggc aggcgagcgg tgccgtcggc
     6541 cagcgctgtg gtcatcagcg ccagcccggg aacgataacg ccgccgcgaa agcatgcctc
     6601 gccgccgacg acatcgagcg catcgatcgt cgtcgccgta ccggcgcaga cgatcagccc
     6661 cggcgcgcgc aggcggcgcc agctgccgat cagcgctgcc cagcggtcga cgccaagctg
     6721 cgtcggccgc gcgtagccgt tgcggacgcc gcagcaatcg gcggcagcgc tgaagtaacg
     6781 gactgcaaaa ccggtggcag cgagtgcggc gctgagtgcc tgctcgcgtt cgggcgcagc
     6841 aacgcaggcg gcgaggactt cgccggcggc accagcggcg acgagccggc ccggcagctg
     6901 atcgagcgcc gcgtagtcga gcgcgccgtg cgctacccac tccggcgtat cgccatcgag
     6961 gcggccgacg ccccacttga gccgactgtt gccgagatcg cagaccgcgt gcagcgggga
     7021 atccacggtc aacgcggcgc ctttggccgc agcgatacgt cgccagcgag cacgcgttcg
     7081 cggccgactg cggtctcaag ccacagcgca ccttcgccgt cgacgccgcg acagatgccg
     7141 tcgcgcttcg taccgtcgtc ctgccgcagt tgcacgagct ggtcggcgaa ggcgttctgt
     7201 gccgaccagg cttcggcgta gaccgggaag cctgcctcgg cgaaacgatc gaggaccgcc
     7261 accatttcgc gcagcagcgc ggcaagcagc tggtggcgct cgggcggcag cgcgatgtcg
     7321 gcgagacccg cggcgggaaa atcgaggcct tcgagaccgt cgggcacttc aaggttgagg
     7381 ccgacgccga tcaccgcggc aacgccggca gcgcctcccg atagctcgat gagcacgccg
     7441 ccgaccttgc cccagccgcc gtcgaccggc agcaggatgt cgttcggcca tttcagccgc
     7501 gcctgcggca cgccgcaggt ggctaatgcc cgcgtgatcg ccacgccgat tgccagcgac
     7561 aggccggaga ggcgcgccga ctgcggaaag cgccagagca gcgaaaaggt caggctggac
     7621 tgcggactgg ccatccagct gcggccgcgc cggccgcggc ccgaggtctg ccggtcacag
     7681 acgacaacgc tgccgctggc cgcgcccttt gcggcgcttg ccagcaggat ggcgttgctc
     7741 gaatcgcaga tcggcagagc gctaagtgag agatgcgccg ccaaggggcc gagccgggcg
     7801 acgagccgct cggtatcgat cagcgcgggt tcggcgggta tggtcatggc ggcgattgtg
     7861 ccatcgcccg gcacgcagcg acgcaccccg gcggcaatcg ctcaggcttc ttcgctacgt
     7921 accggctcgc cgtcgaggcc gagttcgtgg gtcttgcgcg tcagtgtgtt gcgcccaatg
     7981 ccgagcagct gcgcggcctc gatgcggcgg ccgccggtgc tggcgagcgc gcgcgtgatc
     8041 agcgtcttct cgaactcgcg gatcaactgc tcgtgcacgt gaccgcccgc ttcggcgagc
     8101 aggcggtcga cttcggcggc gagcgcggcg acccagctgc cggacgcgct gaccgcgccg
     8161 ccctcgccat cgcgcaggtc ggccggcagg tcggcgacct cgatcgtctg gccgggtgcc
     8221 atcacggtca gccagtggca tatgttctcg agttggcgca cgttgccggg gaagtcgagg
     8281 gtagccaggt ggcgcagcgc cggctcagag aggcgcttcg attcaacgcc cagtgtctgc
     8341 gcgctgcgcg tcatgaagtg ccgggccagc agcgggatgt cctcgcgacg ctcgcgcagg
     8401 ctgggcaggc gcacgcgaat gacgttgagc cgatgcagca ggtcctcgcg gaacaagccg
     8461 tccttgaccc gcccctcaag attctgatgg gtcgcggcga tgatgcggac gttggccttg
     8521 atcggctgat gaccgccgac gcgatagaag tggccgtcgg aaagtacgcg cagcaggcgc
     8581 gtctgcaact cggtcggcat gtcgccgatt tcgtcgagaa acagcgtgcc gccgccggcc
     8641 tcctcgaagc gcccgcgccg ctgcgcctgc gcgccggtga aggcaccgcg ctcgtggccg
     8701 aacagctcgg actcgagcag gtcgcgcgga atcgccgcgg tgttgatggc gatgaacggc
     8761 gcctccttgc gcggactgtg gcgatgcagc gcgcgggcaa cgagttcctt gccgctgccc
     8821 gactcgccgg tgatcaacac ggtggcgtgc gactgggcga ggcggccgat ggcgcggaag
     8881 acctcctgca tggccggcgc ctggccaagg atttccggtg tctcgccgac ctcgtcttgc
     8941 gcaccattct ggtgcgcacc ctcgtcgatc gcgcgccgga tcaggtcgat cgcctggtcg
     9001 acgtcgaagg gcttgggcag atactcgaag gcgccgccct gaaaagcggc aaccgcgctg
     9061 tcgaggtctg agtaggcggt catgatgatg atcgggagcg ccggttggcg ctgtttgagc
     9121 gtcttcagaa gctcgagtcc cgactggccc ggcatgcgca cgtcggacat caccacctgc
     9181 ggtacgtcat caccgcgatc gagctgcgcc agcgcctcgg ttcccgaggc gaagctgacg
     9241 aagggaatct gctcgcgcgc cagcgccttt tcgagcaccc agcggattgc ccggtcgtcg
     9301 tcaatgatcc aaacgggttt catcgtcttt cctgtcatgc gccaacccgt ctcaatgcaa
     9361 gatctcggcc agaagcacta ctgctcacgc cttgcgcgca gcattcggcc gccggtcgac
     9421 cggcaggata acgcgaaaga ccgtatcgcc gggctcgctc tggcattcga tcgaaccgtg
     9481 gtgctgttgg acgaagcttt gcgccaaggt cagcccgagg ccggtgccgt cgtcccggcc
     9541 ggtcaccagc ggatagaaga tgctgtcacg aatttccggc gggattcccg gcccgttgtc
     9601 ggcaacctcg atggcaactg ccaagcgata ccgccgcttg gccagcgtca cctgccgcac
     9661 cgcgcgggtg cgcagcacga tctcgccgtg gccgcggacc gcctgcgcgg cattgcgggc
     9721 aatgttgagg aagacctgga tcagctgttc gcggtcggcg atcaggtcgg gcaggctggt
     9781 gtcgtagtcg cgcgtgatca ccaggccctc ggtgaactcg gcgagcagca ggctgcgcac
     9841 gcgttcgagc acctcgtgca cgttgatctc ggccggatgc atcgcgcggt gtggcgaaag
     9901 caggcgctgc atgaggttct gcaggcggtc ggcctcgtcg atgatcacct gcgtgtactc
     9961 gcgcagcgcc tcgctctgca actcatgctc gagcagttgc gcggcaccgc ggatgccgcc
    10021 cagcggattc ttgatttcgt gcgcaaggtt acggatcagc tcgcggttgg tttgctgctg
    10081 ctcgagcatg cgctcctcgc gactcgcccg gatctgctgg tcgatcggtc tcagctcgag
    10141 aaccagactg ccggcggcgc tgctgatcgg ggtcacggtg caatcgaggt gaatcgaggg
    10201 cgcatcgccc ttgcgccact caatgttgtg gccggtatag ctccagttgt tgtgacgcgc
    10261 gttgtcgagc gcggctgcga ggctggccgt gtagccgaac aagtagttga gtggccgccc
    10321 gcgcagttgc gcgaggctgg tctgcagcag gttctcggag gcagcattgg cgtagcgaac
    10381 ggcgagttcg tcgtcgagga cgacaacggc cgacgagagc agatctagcc cgcgaaaggc
    10441 gtcggcgggc ggcgtgagtg gcggcgacat ggtcactagg cgagcaagaa gcagaccgcc
    10501 ccggcggccg gctcagggca gccgggaaat ctcgcggttc aaggactcga tgttgcgctc
    10561 gtgctgggcg accttgtcgc ggaaggggcg gagtcgctcc tcgcgtttgc cggcattgat
    10621 gccaccgggc accgctgagc agttcatgcc gccgctggca gcgggcacgc agcgctgcgt
    10681 gacattgcgc tcgtcaggct cgatcaccgt ttcctgctcg gcgagctttt cccgtgcctc
    10741 ggccagcgcg cgcgcctcgc cttcgagttc gtcctcgagg atgccgcggc gcatgtcgtc
    10801 gcgggcccgt tgggtcgcct cgtcaacacg cgggaaaccc tcgcgcgccg ccggcgccgg
    10861 acgcgcgacc ggcgccggcg ccgagaccgc cggctggatg acgatcttgc ggcagccctt
    10921 gctcgaggaa ttggagtagg tgacgtgacc atccgcatcg acgcatttgt acatgtccgc
    10981 gaacgccgct gcgggcagcg cggcgaggct ggcaaagagg atcggcatga cagttttcat
    11041 gacacagcag attccacgaa aagtcctgaa agcatacaca caaggcccgg atttacaaag
    11101 aaaaaagggc ggccaggccg ccctttccca cgccttgcgg cgactctgcc aagactcagc
    11161 agctgtagta catgtcgtac tcgaccgggt gcgtcgtcat gcggatcttg ttaacctcgt
    11221 ccatcttcag cgcgatgtag gcatcgatca tgtcgttgga gaagacgccg ccacgggtca
    11281 ggaactcgcg gtcctggtcg aggcagtcca acgcctggtc gagcgcgtgg cagacggtcg
    11341 ggatcttcgc atcctcttcg ggcggcaggt cgtagaggtt cttgtcggcc ggatcgcccg
    11401 ggtggatctt gttctggatg ccatcaaggc ccgccatcag cagcgcggcg aagcacaggt
    11461 aggggttggc acccggatcc gggaagcggg tctcgatgcg gcgcgccttg gccgacgcga
    11521 cgtgcggaac gcggatcgaa gccgagcggt tcttcgccga gtaggccagc ttgaccgggg
    11581 cctcgtagtg cggaacgagg cgcttgtacg agttggtcag cgggttggtg atcgcattca
    11641 gcgcacgggc gtgcttgatg atgccgccga tgtagaacag cgcggtttcc gacaggccgg
    11701 cgtaaccgtt gccggcgaac aggttctgcc cgcccttcca gatcgactgg tgcacgtgca
    11761 tgcccgagcc gttgtcgccg acgatcggct tgggcatgaa ggtcgcggtc ttgccgtact
    11821 ggtgcgcaac gttgtggacg acgtacttga ggatctgcgt ccagtcggca cgacggacca
    11881 gcgtgttaaa aacggtcccg atttcgcact gaccggcggt cgccacctcg tggtgatgca
    11941 cttcgacggg tacgccgacg ccctcgagcg ccatgaccat cgccgaccga atgtcgtgca
    12001 gcgaatcaac aggcgggacg gggaagtaac cacccttgac ggtcggacgg tgaccggtat
    12061 tgccgccgtc gatcttctcg gcagtcgccc aggcggcctc cgaggagaac accttgcagt
    12121 aggaacccga catgtcgacc gaccactcga cgctgtcgaa aatgaagaac tcgggctcgg
    12181 ggccgaagta ggcggtatca ccgacgccgg aggacttcag ataggcctcg gcacgtttgg
    12241 cgatcgaacg cggatcgcgg tcgtagccct tgccgtcggt cgggtcgacc acgtcgcagg
    12301 agacgacgac cgtcgtctcg tccatgaagg gatcgatgta ggcggtcacc gggtccggca
    12361 tcagcagcat gtcggaagcc tgaatgcctt tccagccggc gatcgacgag ccgtcgaagg
    12421 cgtggccgct ctcgaacttg tcctcgtcga acgcgctgac cggcacgctg acgtgctgct
    12481 ccttgccgcg ggtgtcggta aaccggaaat cgacgaactt gacgtcgttt tccttgacca
    12541 tcttcatcac gtcctgaacg ctagctgcca tacgaatctc ctctttcatc gaatcggctg
    12601 ccgggtgctt cccgggtcgc ctggcaaatg gggccgtcaa attgggccat gacccgcagc
    12661 agaaaacatg ccacgccatc cgacgggagg cgcattgtgc cacgcgggcc gctgtgcagg
    12721 cgctcgcgcg atcaccggga cacctcccgg catcatgccg ccgcctggcc gcacttcatt
    12781 ggtgctgact ggcgccgtta ttgcaccgta ttggtgcgtt cgcctgatgg aggcatacgc
    12841 tcaccagcag gcgctgggcc tcgagacgct tgcccagcac tgtctgccat tccgccagct
    12901 gctcgctgct gacggcgtcg gccgcaaaga acacctcggc ctcgatcccc gcctgcagat
    12961 agtgaacaac cacccgcacc ggcgcgggca tgccgcccag cagttcgtcg agcgccgccc
    13021 gcacctgatg acgcgccggc ggccggccgc tcggcggctc ggtgtcatcc tcgggatcga
    13081 tgtgcaccag cacgtcgagc acctgcggct gtgtcctcag cacgcgggca cgtgccgact
    13141 cggcgatgtg atgcccttcg gaaacgctga tccgcgaatc gacctgcaga tgcgcctcga
    13201 ccagcgcgcg gtgcgccatc cgccgggtct tcagctcgtg cacgccgcgc acgccgggcg
    13261 tctcgcgcag cacggcgcgg atcttcgcca cttcctcggt gtcgagtccg gtgtcgatca
    13321 gctcggtcat cgccgaccag gccagttcga cgccgagctt aaggatcatg ccgcccatca
    13381 ggatcgccgc aagcagatcg aggaaggtcc acccgagcag gctgccgcca acgccggcga
    13441 cgacgaccag cgccgacgcg gcgtcggcgc gcgtgtgcag cgcattggcg gcaagcatct
    13501 tcgagcgcag ccggtcggca acggcgatca gataacggta cagcccttcc ttggcgatca
    13561 cggtaatgac cgcgacccag aacgccagct ggccgaccgg gaccgccgtg tcgaccacgc
    13621 gcagcttgcc gaccgcatcc cagaggatgc cgatgccgac ggcgaacaga gaaatgccaa
    13681 gcagcagcgt cgccgccgtt tcgacgcgcg cgtgcccgta gggatgatcg gcgtcggcgg
    13741 gctgatgacc gtggcggttg gcgtagagca ctaggaagtc ggaaagcagg tcggaggccg
    13801 agtgcaggcc gtgcgcgatc agcgattgcg aatgcgcgag ccagccgacc agcagctggc
    13861 cgagggtcag cgccgcgttg acggcagcgc tgatccaggt cgcccttgcg gcaacccgca
    13921 tgcgatcagg cgccgccagc ccactgtccg acgaaccggc ctcgtcggcc ccgccatcgt
    13981 tcaccatcaa ccgcctccat tgccggcgcg caggatcgcg cgccgttttc taagctttat
    14041 actgcaaggc cgactccctg accgaagaaa tccacgatga gctcgctgca agaaatcctg
    14101 accacggcgc agaaccgcgc gcgcgaactg aagctcccct acgagggcgc cgtcacgcca
    14161 cgcgaggcct tcgagctgat gcagcgcgcg cccggcacgc atctcgtcga tgtccgttcg
    14221 aaggccgaac tcgactgggt cgggcggatt cccggggcgg tcgaaattga atgggtaagc
    14281 tatccgggaa tgacgcgcaa cccgctcttc ctcgatcacc tccggcgtca ggtcgacagc
    14341 gaaggactcg tactcttcat ctgccgcagc gggcagcgct cgcacttcgc cgcggcggtc
    14401 gccaccgagg cgggtttcgc cgactgctac aacgttctcg aaggcttcga gggcgagaag
    14461 gacaccagcg cccaccgcaa cacggtcggc ggctggcggg tcgccggtct gccctggata
    14521 caggggtaag ccgaccggcg ttgagaaccc cgcgccacag gctggggtcg aacactgtgc
    14581 attagctgac aggcgggttg acacgctgcc cgccattact accttagtaa tagtcaccgt
    14641 ggtaaattcc gtcgccttgc cgagatgggt cagaccggaa ttcgccatta ccggaactga
    14701 agacatgacg ccgccgacca ctaccgaaac cgccccactg cctttcgcgc cctacaacgc
    14761 gatggacgac gctgcctgcg aggtacgcat ccgcgaagcc cgcgccaaac tcggcaagcg
    14821 cgcggtgatc ctctgccacc actaccagcg cgccgacatc taccggcacg ccgacatcac
    14881 cggcgactcg ctcaagctct cccggctcgc ctcgcagtcc gatgccgagt tcatcgtctt
    14941 ctgcggcgtg cacttcatgg ccgaagtcgc cgacatcctc tccaagccgc agcagcaggc
    15001 gattctgccc gacctggctg ccggctgttc gatggccgac atggccaacc tggccaaggt
    15061 aacgcgctgc tggcgcgaac ttggcgaagc gctggggaac cccgacgagg cgatcacgcc
    15121 ggtcacctac atcaactcgg ctgccgacct caaagccttc tgtggcgagc acggcggcat
    15181 cgtctgcact tcatcgaacg cccgcacgat cgccgagtgg tccttcggca agcggccgaa
    15241 gatactcttc tttcccgacc agcacctcgg ccgctggacc ggccaccagc tcggcctgcc
    15301 tctcgaggac atgcgcgtct gggacccgga cctaccgatg ggcgggctta gcgttgacga
    15361 gatcaaggcc gcgcgcatcc tgctctggaa ggggcactgc tcggtgcacc agatgttcca
    15421 gctgaagcac gtgcaggcgt tccgcgccaa gtatcccgac ggacaggtga tcgcccaccc
    15481 cgaatgcaac ttcgaggtct gcgccgccgc cgaccacgtc ggctcgaccg aatacattgt
    15541 caagacgatc aaggcgtcgg cgccgggcac gcgctggctg gtcggaaccg agctcaatct
    15601 ggtcagccgg ctggccgaag aggaggcgcc gcgcggcgtc accgtgcagt tcatggcgac
    15661 cacggtctgc atgtgctcga cgatgcagcg catcgacccg cagcatctcg cctggtgcct
    15721 cgacaacctg gtcgccggcc acgtcgtcaa cgccatttcg gttcccgatc atgaggccaa
    15781 gctcgcgcag ctggcgctcg accggatgct ggcgatttca tgatccccga cgcggacgag
    15841 ccctctcact tccttaattg cctcgacgac ttccggctcg gccacgaagt cctcgaccgc
    15901 gagcacgagg ggctgtgtct gctgatcaac cagcttgccg aagcggtcga cgatcaggac
    15961 gactgggaga aagcggtcgc gctcgccacc gaactcgttc acgaaaccag ccgccatttc
    16021 gaccacgaag aggcgctgat gaagcaagcg gcctatcccg aggtcgatcg gcaccacgag
    16081 cagcacaccg aactcctgtc gcagattgaa tcgctgcgcg accgcctcgc cacagaccac
    16141 cgcctcgctc gaccgcacca gacgcttaac ttcctcggcg actggttcac gctgcacatc
    16201 gacaactccg atcgccgctt cgtcgccttt ctaagcgaat cctgaaccgt gcggactcgc
    16261 aggacgtgcc tgcgcgttgc cggccgacgc ccgcatcggc gagaatgacg gcaccattgc
    16321 caccgatggc tgaggccaga gcgccctacc ggcgcaggcg gccatttcga acctgacggg
    16381 acaaagcacg cgcgcgatga ccgacaccgg actgcacgtc gccacctaca acatccacaa
    16441 gggcttctcg cagttcaacc ggcgcatggt cgtgcacgac ctgcgcgaca ggctgcggac
    16501 gctgtccccc gacctgctgt ttctgcagga agtccaaggc gagcaccatc accatgccgc
    16561 gcgccacgcc gactggccca cgacggcgca gcatgagttc atcgcggagg acgtctgggc
    16621 caatagcgcc tacggccgca acgtcgtcta cgaccacggc caccacggca acgccattct
    16681 ctcccggcac ccgctcgccg acagccacaa ccaggacgtc accgacctcg ccttcgaacg
    16741 tcgtggtctg ctgcatacgc agatgcatct cccaggatcg gcgcagccga ttcattgcgt
    16801 ctgcgtgcac ctgtcgcttc tgggccgttc tcgtcgtcgc cagctggaac tgctcgccga
    16861 gcggctcgat cactgggcgc ccggcgatgc gccactgatc gtcgccggcg atttcaacga
    16921 ctggcgcaac cgtgccgacc cgctgttcgc acgcaagctg gggctttcag aagccttcgg
    16981 cggtgagagt ggtcgtccgg tgaaaagctt cccatcggca atgccggtct ttcgtctgga
    17041 ccgcatctac gtccgcggct tctcggttga atcggcccgc gtgcatagcg gacgaccgtg
    17101 gtcgcggata tcggatcatg cggcactatc ggctgtcctg aggccagtgg catgaggcgc
    17161 gctgcagcca ccaccgtcgc gtcacgatga aggcgcactt cgtccgcggc aaccgcctga
    17221 cgctgctggc cggcggcgcg gccttctttc gtgaactgcg cgcagcaatc gccgacgcgc
    17281 agcacgaagt gcacctagag acctacatct acgccgacga cgaaacaggc caggcggtca
    17341 ctgctgaact cgtggcagcc gccgcgcgtg gcgtactggt ccgcctgctg gtcgacggct
    17401 tcggcagcaa acccttcgcg caggcgcaca tggcacggct gcgcgacgcc ggcgtgcaga
    17461 tgatggtcta ccgccccgat atttcgccct acgcgctgcg gcgccggcgc ctgaggcgtc
    17521 tgcaccgcaa gctggcgatg atcgaccgct gcacggcctt catcggcggc atcaacatcg
    17581 tcgacgaagt gccgctcggc atggcggcgc cacgcctcga cttcgccgtg aagatcgaag
    17641 ggccgctgct tgcgccgatt caccacagtg cgcaccgcat gtgggagatc gtcagctggg
    17701 ccaattttcg ccggcgatac cggctcgccg ccaacgaagt cggctgcagc gccccgagcg
    17761 aaccgatcgg caaagcgact gcggcgctcg tgctgcgcga caacgtccgc cggcgccacg
    17821 acatcgagga tgcctatctc gaggcgatcg aggcggcacg ccacgacatc ctgattgcca
    17881 atgcctactt cctgcccggc cggcgctttc gccgcgccct gctcgacgcg agcgcccgcg
    17941 gcgtcaccgt ccgcctgctg ctgcaggggc acgccgaata ccgcctgcaa cacctggcca
    18001 cgca
//
LOCUS       DATDAA010000015         2645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_245510,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000015 DATDAA010000000
VERSION     DATDAA010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245510"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VIS73_00570"
     CDS             complement(<1..636)
                     /locus_tag="VIS73_00570"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5761677.1"
                     /translation="MDNLSLLIQAPVLNVQLLIDGLCIGATFALAAYGLAMVWGVMNV
                     KNLCQGDMVILGGFLAVTLANNGVHPIFGLPLAIVVLFVYGWVVYRLIICRVIERDLF
                     TSLLATFGLAIVMAQSMNLIFGPEVVTADSGFESRQLFGGLVTIAEIKLISLGLAAIL
                     AAIVIVFMKRSRMGQAIRATAQDARAARVMGIDTDRVYQFTYALNAAICGAA"
     gene            complement(714..1949)
                     /locus_tag="VIS73_00575"
     CDS             complement(714..1949)
                     /locus_tag="VIS73_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5761678.1"
                     /translation="MKQAFYFKRISCAAATAVALAFASSAVAKVEGDEIILGAAISLT
                     GKYSSNGVNAKNGYDIAVKRINEMGGVKVGGKSYKMKVVYYDDESTPARGAQLAERLI
                     QQDGVKFMLGPYSSGLTKAIAPVTEKYKIPMVEAEGASRSLFTQGYRYMFAVLSTSEQ
                     YLGSAIDLKAEVAKKAGKNPKDVKVAMVFENDPFSLDVRAGCVEDIKRHGMKIVIDDK
                     MPRDLNDISPTLTKIKALKPDLILISGHEKGAATAARQMKEMQVKAPMVGITHCESGK
                     LISQFGKSVEGYLCPTQWDETLSYKDNYFGSAADFDKHVKATYSQYKVVPYQLAQASA
                     AVLVWKDAFERANSFDTEKLRDAIAATEMETFYGNIKFSKAGNNVAKPMVLRQIQGGE
                     LKVVAPTRWAAVPLVYPLK"
     gene            complement(2083..>2645)
                     /locus_tag="VIS73_00580"
     CDS             complement(2083..>2645)
                     /locus_tag="VIS73_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761679.1"
                     /translation="QFISKLSLIASGLTMLGMFVFRRFIADHSIAYIVVFLTVIGTVL
                     SLPNIGMYYGLHEWTAAHTGGVVDARFIAVIDTALESPLGQVAMIPMLAWIANSAPAR
                     LKATFFAVMASFTNLALSASQLGTKYLNRLFSVTREVRDPVTQAVVVAEDYGELGWLL
                     MAAALIGFTLPMLAILAVRVFRWRTA"
BASE COUNT          497 a          833 c          818 g          497 t
ORIGIN      
        1 tgcggcgccg cagatcgccg cattcagcgc gtaggtaaac tggtagacgc ggtcggtatc
       61 gatgcccatc acgcgcgcgg cgcgcgcgtc ctgggcagtg gcgcggatcg cctggcccat
      121 gcgactgcgt ttcatgaaca caatgacaat cgcagcaaga atggccgcaa ggcccaggga
      181 tatcagcttg atttcggcga tcgtcaccaa tccgccaaac agctgacgcg actcgaaccc
      241 ggagtcggcg gtcaccactt cgggcccgaa gatcaggttc atgctctgtg ccatgacgat
      301 cgccagcccg aaggtcgcga gcagcgacgt gaaaagatca cgctcaatga cccggcagat
      361 gatcagccgg tagaccaccc agccgtagac gaacaatacg acgatcgcca gcggcaggcc
      421 gaagatcggg tgaacgccgt tgttcgccag cgtcacggcg aggaaacctc cgaggatcac
      481 catgtcgccc tggcagaggt tcttgacgtt cattacgccc cagaccatcg ccagcccgta
      541 ggccgcaagc gcgaacgtcg cccctatgca cagcccgtcg atcagcaact gcacgttgag
      601 gacgggtgct tgtatgagca agctcaggtt gtccatgcgg tccccttcgg atgagcgagt
      661 ccctgccgcg gcggcgcgcg gcagggctcg tcacaagctt atggcgcaat accctacttg
      721 agcgggtaga ccagcggcac cgccgcccag cgggtcggtg ccaccacctt cagctcgccg
      781 ccctggatct ggcgcagcac catcggcttg gcgacattgt tgcccgcctt ggagaacttg
      841 atgttgccgt aaaaggtctc catctcggtc gcggcgatcg cgtcgcgcag cttctcggtg
      901 tcgaacgagt tggcccgctc gaaggcatcc ttccacacca gcacggccgc cgaggcttgc
      961 gcgagctgat agggaacaac cttgtactgc gagtaggtcg ccttgacgtg cttgtcgaaa
     1021 tcggccgcgc tgccaaagta gttgtctttg tagctcaggg tctcgtccca ttgcgtcggg
     1081 cagaggtaac cttcgaccga tttgccgaac tgcgagatca gcttgccgga ctcgcagtgc
     1141 gtgatgccga ccatgggcgc cttgacctgc atctccttca tctggcgcgc ggcagtggcc
     1201 gcgccctttt cgtggccgga gatcaggatc aggtcgggct tgagcgcctt tatcttggtc
     1261 agcgtcggcg agatgtcgtt caggtcgcgc ggcatcttgt cgtcgatgac gattttcatg
     1321 ccatggcgct taatgtcctc cacgcagccc gcccgtacgt ccagagagaa cgggtcgttc
     1381 tcgaagacca tcgccacctt cacgtccttc gggttcttgc ccgccttctt cgccacctcg
     1441 gctttcaggt cgatcgcgct gcccaagtac tgttcggagg tggagagcac ggcgaacatg
     1501 taccggtagc cctgcgtaaa gagcgagcgc gaggcgccct cggcctcgac catcgggatc
     1561 ttgtatttct cggtcaccgg ggcgatggcc ttcgtcagac ccgagctgta ggggccgagc
     1621 atgaacttga caccatcctg ctgaatcagg cgctcggcga gctgcgcgcc gcgtgcgggc
     1681 gtcgactcgt cgtcgtaata gacgaccttc atcttgtagg acttgccacc gactttgaca
     1741 ccgcccatct cgttgatgcg cttgacggcg atgtcgtagc cgttcttggc gttgacgccg
     1801 ttggacgagt acttgccggt gagcgagatc gccgcgccaa ggatgatctc atcgccttcg
     1861 actttggcga ccgccgacga cgcgaaggcg agcgcgaccg ccgttgctgc ggcgcagctg
     1921 atgcgcttga aatagaacgc ttgtttcatt acctgtcctc ctccaagtag gtatcgcggt
     1981 catgggatcg cgagggcgaa acattagcac agggcggcgg cgtgggaaac tgcatccgct
     2041 gctcgtggac ggctcatgca tgagccactc ggcgcggcgt gcttatgccg tgcgccagcg
     2101 aaagacccgc actgcaagga ttgccagcat gggcagagtg aacccgatga gcgcggccgc
     2161 catgagcagc caaccgagct cgccgtagtc ctcggcaacc acgaccgcct gcgtgaccgg
     2221 gtcgcgcact tcgcgcgtta cgctgaacag ccgattgagg tatttcgtcc ccagctgcga
     2281 cgccgagagc gcgaggttgg tgaacgacgc catcaccgcg aagaacgtcg ccttgaggcg
     2341 cgcgggagcc gagttggcga tccacgcgag catcgggatc atcgccacct gtccgagcgg
     2401 agactcgagg gccgtgtcga tcaccgcgat gaagcgcgcg tcgaccaccc ctcccgtgtg
     2461 tgccgctgtc cactcgtgca gcccatagta catgccgatg ttcgggaggc tgaggaccgt
     2521 gccgataacg gtcaggaaca cgacgatgta ggcgatcgaa tgatccgcaa tgaagcgccg
     2581 gaagacgaac atccccagca tggttaggcc gctcgcaatc agcgacaact tcgagatgaa
     2641 ctgct
//
LOCUS       DATDAA010000016         2281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_106088,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000016 DATDAA010000000
VERSION     DATDAA010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106088"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VIS73_00585"
     CDS             complement(<1..366)
                     /locus_tag="VIS73_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEY5761680.1"
                     /translation="MCGRFAFYSPSEAVARLFGASGARQLKPRYNIAPTQYVAAIRNA
                     GDDGRDLVELRWGLVPFWAKDPSIGNRMINARAETVAEKPSFRNAYRKRRCLVLADGF
                     YEWHREGEVKVPYFISLASG"
     gene            complement(372..1145)
                     /locus_tag="VIS73_00590"
     CDS             complement(372..1145)
                     /locus_tag="VIS73_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5761681.1"
                     /translation="MNWRVNRIAFQTLVRKEIVRVVRIWVQTIVPPAITMTLYFIIFG
                     NLIGRRIGVMDGFDYMQYIAPGLIMMSVITNSYGNVVSSFFGAKFGKHIEEMLVAPIS
                     NATIIIGHIAGGVIRGLMVGSLVTVVALFFTRLHVEHPFVTLSMVLLSSTVFALLGMV
                     NAIFAKKFDDISIIPTFVLTPLTYLGGVFYSISLLPDFWQKVSLGNPILYMVNAFRYG
                     ILGVSDIDIGWAYLIVGVFVAGLFTLNLVLLNRGVGIRE"
     gene            complement(1142..2077)
                     /locus_tag="VIS73_00595"
     CDS             complement(1142..2077)
                     /locus_tag="VIS73_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5761682.1"
                     /translation="MRALAIKNLRKTYSNGVEALKGIDFNVESGDFYALLGPNGAGKT
                     TAIGIITSLVNKTAGKVEVFGHDLDSDLEAAKACIGLVPQELNMNQFDSVGNIVLNQA
                     GYYGIDRRTAVERAEKYLSELRLWDRRKEPARNLSGGMKRRLMIARALIHEPKLLILD
                     EPTAGVDIEIRRSMWNFLRRINRDGTTIILTTHYLEEAESLCRHVAIIDEGRIIEDAR
                     MSDVLRKLQREVFVLSLGEPLARAPELPGFEAELNDEYELEVALYAKDDLNTLFRCLD
                     ERGIRVTSLRNKANRLEELFLGLVEGKEVQGGSRG"
BASE COUNT          437 a          704 c          715 g          425 t
ORIGIN      
        1 gccgctggcc agggatatga agtaaggcac cttgacctcg ccttcccggt gccactcgta
       61 gaaaccgtcg gccaggacaa ggcagcgtcg tttccggtaa gcgttacgga aagagggctt
      121 ttcggccacg gtttcagccc gggcgttgat catgcgattg ccgatcgacg ggtccttcgc
      181 ccagaacggc acgagccccc agcgcaactc gacgaggtcg cggccgtcgt cgccggcgtt
      241 ccggatggcc gccacgtact gggtcggggc gatattgtag cggggcttca gctgtctcgc
      301 gccgctcgcg ccaaacagcc gggcgactgc ttcgctcggc gaatagaagg caaatcgccc
      361 gcacatgtcg cctactcccg gatcccgacg ccgcgattca gcagcacgag attgagcgtg
      421 aacaggccgg ccacgaagac gccgacgatc aggtaagccc agccgatgtc gatgtcggac
      481 accccgagaa tgccgtagcg aaaggcattg accatgtaca ggatcggatt gcccagcgac
      541 accttttgcc agaaatccgg cagcagggag atcgagtaga aaacgccgcc gagataggtg
      601 agcggcgtga ggacgaaggt cgggatgatc gaaatgtcgt cgaacttctt ggcgaagatc
      661 gcgttcacca tcccgagaag ggcgaagacc gtcgacgaca gcaggaccat cgacagggtg
      721 acgaaaggat gctcgacgtg aagccgcgtg aagaacagcg caacgaccgt gaccagcgaa
      781 ccgaccatga ggccgcgaat cacgccgccc gcgatgtggc cgatgatgat ggtggcgttg
      841 gaaatcggcg cgacgagcat ttcctcgatg tgcttgccga acttggcgcc gaagaacgag
      901 gagacgacgt tcccgtacga gttggtgatg accgacatca tgatgaggcc cggcgcgatg
      961 tactgcatgt agtcgaatcc gtccatgacg ccgatgcgcc ggccgatcag gttgccgaag
     1021 atgatgaagt agagcgtcat cgtgatggcg ggcgggacga tggtctgcac ccagatacgc
     1081 acgacgcgga cgatctcctt tctgaccagc gtctggaacg cgatacgatt gacgcgccag
     1141 ttcatccccg gctcccgccc tgcacttcct tgccctcgac gaggcccagg aatagttctt
     1201 cgagacggtt cgccttgttg cgcaggctcg tcacccggat gccgcgctcg tccaggcatc
     1261 tgaacagcgt attgaggtcg tctttcgcgt acagggccac ctccagctcg tattcatcgt
     1321 tcagttccgc ctcgaagccc ggcagctccg gcgctcgcgc cagcggttcc ccgaggctca
     1381 gtacgaacac ttcgcgctgc agcttgcgca gcacgtcgct catgcgcgcg tcctctatga
     1441 tgcgaccctc gtcgatgatc gcgacgtgcc gacagaggct ctcggcttcc tcgagataat
     1501 gggtcgtcag gatgatggtc gtgccgtccc tgttgatccg gcggaggaaa ttccacatgg
     1561 agcgccggat ctcgatgtcg acgccggcgg tcggctcatc gagaatcagg agtttaggct
     1621 cgtgaatcag ggcccgggcg atcatcagcc gccgtttcat gccgccggac aggtttcgcg
     1681 ccggctcctt gcgccgatcc cacaggcgga gttcggagag gtatttttcg gcgcgctcca
     1741 cggccgtccg ccgatcgatg ccgtagtagc cggcctggtt caggacgatg ttcccgaccg
     1801 agtcgaactg gttcatgttc agctcctggg gaacgaggcc tatacaagcc tttgccgctt
     1861 cgagatcgct gtcgagatcg tgaccgaaaa cctcgacctt gccggcggtc ttgttgacca
     1921 gcgacgtgat gatgccgatc gccgtggtct tgccggcgcc attgggcccg agcaaggcat
     1981 aaaagtcgcc cgactcgacg ttgaagtcga tgcccttcaa ggcctccacc ccgttcgagt
     2041 aggtcttcct gagattcttg atcgccagcg ctcgcattgc gccggtattg tatacatctt
     2101 gactaaaact gtgggtcgtt ttgatcggcg acccgcagcc cgggcgcgtg caatcgactc
     2161 gcggcccgca ggcgcacgct gcgttcgacc ggcgccgaca gaatcgaatg cagcgcggtt
     2221 aactgcgccg cctggcgcac gtcgggtctc cggttgatgc cgccgccgag cagcaggcgc
     2281 c
//
LOCUS       DATDAA010000017         7390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_350646,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000017 DATDAA010000000
VERSION     DATDAA010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350646"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..520
                     /locus_tag="VIS73_00600"
     CDS             113..520
                     /locus_tag="VIS73_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761683.1"
                     /translation="MSKPERLISRYAEQPGLSGALLFALVAERLAQFYDHGQWPNDGR
                     MRQQALDWLQRAKVKIDSPWLRELVSTSGDMASEISASLSREAGLQTAHEMAESLDPN
                     HPSPIGQMMMAQCVAWIDTMNRAEVATDEDPVR"
     gene            501..890
                     /locus_tag="VIS73_00605"
     CDS             501..890
                     /locus_tag="VIS73_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5761684.1"
                     /translation="MKTRYAEVFPYLTRDGSAIRELMHPAVHGGRNQSLAEAVIPAGD
                     RTQLHFHRLTEEIYHVTAGSGVMRLGDTQFVIAPGDTILIKPGTAHCVVATGEVPLHI
                     LCSCAPAYSHEDTILVDDDPDSTAKPR"
     gene            887..1750
                     /locus_tag="VIS73_00610"
     CDS             887..1750
                     /locus_tag="VIS73_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5761685.1"
                     /translation="MNFQSWIGRVEEATDVVNATPMAALAATLDRPADRPPIGTPLPP
                     LWHWLYFLPLHRQSDLGPDGHAQRGGFLPPVPLPRRMWAGSRFEFHKPLSVGDTLTRR
                     STIDNVTEKQGRSGPLVFVTVRHDIGHTGENDVALTEFHDIVYRDSPKSGDPAPTRQA
                     ADASPTWERQWVPDDVLLFRYSALTFNGHRIHYDRRYVTEVEGYPGLVVHGPLIATLL
                     LDLLREMMPASDVLRYEFRAVRPLFDTQPFFVCGKPQAGGESVRLWARDSDGWLAMEA
                     MVELRPSAKRV"
     gene            1789..2115
                     /locus_tag="VIS73_00615"
     CDS             1789..2115
                     /locus_tag="VIS73_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761686.1"
                     /translation="MDCRCPICSHDLGRRKLGHAIVARMEIECAFCKNYIRLNVHPAE
                     HITVLLGFCGFLVLAAIAYLLQSQALAVIAFGAAMLGAMSLPVLERTYLRNWPRYARL
                     EPKTQP"
     gene            2433..2903
                     /locus_tag="VIS73_00620"
     CDS             2433..2903
                     /locus_tag="VIS73_00620"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEY5761687.1"
                     /translation="MRKYSIDSEAATSRILAAALLADGALNKSELDLIDRHYLIDQLG
                     ISAETLDRAIHEFCDDMNQYARRDDCGDLDLGRQAIDGILDEIRDRNRQKALLSAIIE
                     IVCADRQVSSGEAALASQAMLRWSISPQDIGYELHPFSPRWPKQFDRASMGVTL"
     gene            complement(2962..3912)
                     /gene="nhaR"
                     /locus_tag="VIS73_00625"
     CDS             complement(2962..3912)
                     /gene="nhaR"
                     /locus_tag="VIS73_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HEY5761688.1"
                     /translation="MRQLNYKHLRYFWAVAKAGSVARASERLHLAPHSISGQLRELEE
                     NLGVPLFQRVGRGLELTDMGRRILSYAEEIFALGDELLEVARDQSARQSIPFAVGIAD
                     SVPKTIAYRLVEPALQVEEPIRLICREGRLNALLADLAVHKLDMVIADRAMPSRLNVR
                     GYSHLLGESEVSVCARPELADRFPGAFPARLDGAPFLLPGEDSAIRTGIERWFETNDI
                     HPRIVGEFDDSALLKAFAKAGAGFFATPKAILDVVAAQYGVNDLGLIDGVREELYAIT
                     TERRLTHPAIVAISEGAKLDLFAAPSGRKVAKPSGKTPRA"
     gene            4037..4438
                     /locus_tag="VIS73_00630"
     CDS             4037..4438
                     /locus_tag="VIS73_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEY5761689.1"
                     /translation="MQFDIQSRGFDLTHALRDHVRRRLLLELSWATDHVGKVTVRLSD
                     LNGPRGGPDKRCRIQISALRSSVVVEDVEEDLYIAIGRAADRAGRTLSRRIARHREHR
                     AVRMSRPIEPQSAPATSHLMPPGEPAGATQH"
     gene            4461..5144
                     /locus_tag="VIS73_00635"
     CDS             4461..5144
                     /locus_tag="VIS73_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEY5761690.1"
                     /translation="MANLKTISGHYAGSATTDSVLSTNKVVRNTYLLLSMTLLFAAAT
                     AGASLALNLPHPGLIITLVGYFGLLFLTSYLKDSAWGIASVFALTGFMGYTLGPIVNH
                     YLGLTNGGQTVMMAMAATATIFIGLSAYALTTRKDFNYLGGFLMVGIIVAFVAGLAAI
                     FFEIPALSLTVSAAFVLLMAGLILYQTSSIINGGETNYIMATVTLFVSIFNLFTSLLH
                     LLGFMNSND"
     gene            complement(5141..5365)
                     /locus_tag="VIS73_00640"
     CDS             complement(5141..5365)
                     /locus_tag="VIS73_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761691.1"
                     /translation="MVLRLPDRYSGIGRGTRSGSPQHGATNSPRWLATAARALVDAAG
                     DAGVVVEVNDPGVVCDIDRPRDLFAATPHL"
     gene            5776..6774
                     /locus_tag="VIS73_00645"
     CDS             5776..6774
                     /locus_tag="VIS73_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5761692.1"
                     /translation="MNLNKLSLAVAALLAGVSAAHLAHAADPIVTIDGSSTVYPITEA
                     VAEEFQISKRNKVRVTVGISGTGGGFKKLCRGEVDISGASRPIKKSEMEACKAAGISY
                     YEMPVAFDALTVMIHPSNKFAETMTMAELKKAWAPEAKGTVTTWKQINPAWPDTPMKL
                     YGPGTDSGTFDYFTEAINGKGGASRPDYTASEDDNVLVQGISQDKGALGYFGFSYYWE
                     NRNKVKAVKVDGGKGPVAPSPESVENGAYAPLSRPVFIYVSAKALEKPEVKEFVEFYM
                     ANATTLVKEVKYVPLPAAIYKSNLDHVAKKKVGTVFGGESDVGTRIEDLVKREAKL"
     gene            complement(6829..>7390)
                     /locus_tag="VIS73_00650"
     CDS             complement(6829..>7390)
                     /locus_tag="VIS73_00650"
                     /inference="COORDINATES: protein motif:HMM:NF016843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HEY5761693.1"
                     /translation="LSGRWAVVGYWRKWRGTTAGGPPRVSNSEIGWSWIGAFLGVGAV
                     AWVSSGLLDTIGTSLMIGSFGASAVLLYGAPRSPLAQPRNLVGGHVISALIGVACWKL
                     AGEMPGLAQALAVATAIAVMHLTRTLHPPGGATALIAALGTSDIEQLGFFFAAIPVAL
                     GASILLLVALVVNNLPKSRRYPEIWW"
BASE COUNT         1469 a         2484 c         2143 g         1294 t
ORIGIN      
        1 attcaccggc cagcgatcgc gtatcgctgg tgacaagaag gctaacccgc tcgacgactt
       61 gttcgggcca acggccgatt agtcgactcg atcgaccaag caccgttatc cgatgagcaa
      121 acccgaacgc ctgatcagcc gctacgccga gcaacccggg ctctcgggcg ccctgctgtt
      181 cgcgctggtc gccgaacggc ttgcgcagtt ctacgaccat ggccagtggc ctaacgacgg
      241 ccgcatgcgc cagcaggcgc tcgactggct gcagcgcgcc aaggtgaaaa tcgactcgcc
      301 ctggctgcgc gaactggtct cgacgagcgg cgacatggca agcgagatca gcgcgtcgct
      361 gagtcgcgag gccggcctgc agaccgcgca cgagatggcc gagagcctgg acccgaatca
      421 cccgtcaccg attgggcaga tgatgatggc gcaatgcgtt gcctggatcg acacgatgaa
      481 tcgcgcggaa gttgccaccg atgaagaccc ggtacgctga ggtttttccc tatctcaccc
      541 gcgatggctc ggcgatccgc gagctaatgc accccgcagt ccacggcggc cgcaatcaga
      601 gcctagccga ggcggtcatc ccggccggcg accgcacaca gctgcatttc caccggctga
      661 ccgaagagat ctaccacgtc accgccggca gcggcgtaat gagactgggc gacacgcagt
      721 tcgtgatcgc ccccggtgac acgattctga tcaaacccgg taccgcgcac tgcgtcgtcg
      781 ccactggcga ggtgccgctg cacatcctct gcagttgcgc gccggcgtat tcgcacgagg
      841 acaccatcct cgtcgatgac gatccggatt cgacggcgaa accgcggtga acttccagag
      901 ctggatcggc cgcgtcgagg aggcgaccga cgtcgtcaac gcaacgccga tggccgcgct
      961 cgctgcgacc cttgatcgcc ccgccgacag gccgcccatc ggcacaccgc tgccgccact
     1021 ctggcactgg ctctacttcc tgcccttgca ccggcaatcg gatctcgggc ccgacggcca
     1081 tgcccaacgc ggcggattcc ttccaccggt accattgcca agacgcatgt gggccggcag
     1141 ccggttcgaa tttcacaagc cactgagcgt cggcgacacg ctgacccgcc gctcgacgat
     1201 cgacaacgtc accgagaaac aggggcgcag tgggccactg gtgttcgtca ccgtgcgcca
     1261 cgacattggc cacacgggcg agaacgatgt ggcgctgacc gagttccacg acatcgtcta
     1321 ccgcgactcg ccaaagtccg gtgatccggc gccaacgcgg caggccgccg atgcctcccc
     1381 gacatgggag cggcagtggg tacccgacga tgtcctgctc ttccgctact cggctctgac
     1441 cttcaacggc caccgcatcc actatgatcg tcgctacgtc accgaggtcg aaggctatcc
     1501 cggcctcgtc gtccacgggc cgctgatcgc gacgctgctg ctcgacttgc tgcgcgaaat
     1561 gatgcccgct tccgatgtgc tgcgctatga gttccgggcc gtccgccccc tcttcgacac
     1621 gcagccgttt ttcgtctgcg gcaagccgca ggcgggcggc gaatctgtcc gtctgtgggc
     1681 gcgcgattcc gacggatggc tggcgatgga agccatggtc gaactgcgcc cgtcggccaa
     1741 acgcgtctaa ccgctcaaat agccctaacc gagacctgag gccagaacat ggattgtcgt
     1801 tgtcccatct gcagtcacga tctcggccgg cgaaagctcg gccatgcgat cgtggcgcgc
     1861 atggagatcg agtgcgcttt ctgcaagaac tacattcgac tcaatgtgca tcccgccgag
     1921 cacatcaccg tcctgctggg tttttgcggt ttcctcgtgc tagcggcaat cgcctatctg
     1981 ctgcaaagcc aggcgctggc tgtcatcgcc tttggcgcgg cgatgctcgg cgccatgtcc
     2041 ctgcccgtgc ttgaacgcac ctatctccgc aactggccgc gctacgcgcg cctcgagccg
     2101 aaaacccagc cctagcgctc gcggcgcaac cctcaaccaa cgcggcgccg acgatcgctt
     2161 gactcagacc ggctcacatt gacgcggaac agcgcgactg accgatccga tcgcgccgtg
     2221 ggcgccacgc catcatcagc tgcttcgcca gcgttccaag atgctccgat gttcggcgaa
     2281 gcccgccatg aacggcggca cgacaataca tcgtcttttt cgtattgttt tgcattttta
     2341 ttcgacttgt tcgacttgtc atggccgtct agattgccac tgcaccgcaa atttgcggaa
     2401 cgcgtcagcc aattgcgaac aaaggagatt tcatgcgcaa gtactcgatc gatagtgaag
     2461 ccgcgaccag tcgaatcctc gctgcagcac ttctggccga cggggccctc aacaaatccg
     2521 aactcgacct gatcgatcgc cattacctga tcgatcagct tggcatttct gccgagacgc
     2581 tcgaccgcgc catccatgaa ttctgcgatg acatgaacca gtacgctcga cgtgatgact
     2641 gcggtgatct cgatcttggt cggcaagcga tcgacggaat acttgatgaa atccgtgatc
     2701 gcaatcgaca aaaggcgcta ttgagcgcga tcatcgagat cgtctgtgcc gaccgccagg
     2761 tcagttcagg cgaagcggcg ctggcatcac aggccatgct gcgctggtcg attagcccgc
     2821 aggacatcgg ttacgaattg caccccttct cgccgcgctg gccgaagcaa ttcgatcgcg
     2881 ccagcatggg cgttacgctt tagggaagca gagttccggc tcgcagccgt gcggccggcc
     2941 gggaggctgg gcgatccgct gctaggccct gggcgtcttg ccgcttggtt tcgccacctt
     3001 tctgcccgat ggcgccgcga acaagtccag cttggcacct tcactgatcg caacgatggc
     3061 cggatgggtg agtcggcgtt ccgtcgtgat cgcgtatagc tcctcgcgga caccgtcgat
     3121 caggcctagg tcgttcacgc catactgcgc ggcaaccacg tctaagatcg ccttcggcgt
     3181 tgcgaagaag ccggcgcccg ccttggcaaa cgccttcaac agcgcgctgt cgtcgaactc
     3241 gccaacgatc cgcgggtgaa tatcgttggt ctcgaaccag cgctcgattc cggtgcggat
     3301 ggccgaatcc tcgcccggca acaggaacgg agcaccatcg aggcgagccg gaaaggcacc
     3361 gggaaaacga tcggcaagct cagggcgcgc gcagaccgac acctcgctct cgccgagcag
     3421 atggctgtaa ccacgcacgt tgaggcggct cggcatcgca cggtcggcga taaccatatc
     3481 gagcttgtgc accgcgagat cggcaagcag tgcgttcaac cgtccctccc ggcagatcaa
     3541 tcgaatcggc tcttccacct gcagagccgg ctcgaccagg cggtacgcga tcgtcttcgg
     3601 aaccgagtcg gcaatgccga cggcgaaagg aatgctctgc cgcgccgact ggtcccgcgc
     3661 gacctcaagc aactcgtcac cgagcgcgaa gatctcttcc gcatagctca ggatccgtcg
     3721 gcccatatcg gtcagttcca ggcctctccc gacccgctgg aacaggggaa cacccaggtt
     3781 ttcctcaagc tcgcgcaatt ggccgctgat cgaatgcggc gccagatgca gacgttcgct
     3841 cgcccgtgcc acgctaccag ccttcgcgac ggcccagaaa taccgcagat gcttgtagtt
     3901 caattgccgc atgcgcaccg ctccgatggg ttcaaccaat cgagtttttc gatttgtcgt
     3961 tcaaatatat tcgatttgtt ggagtcgtat caatccgcta gagtgaaatg cctgtaacct
     4021 caaccaagga gtcacgatgc aattcgatat ccagagtaga ggcttcgacc tgacacacgc
     4081 cttgcgcgac cacgtgcggc ggcgcctgct gctggaattg agctgggcaa ccgaccatgt
     4141 cggcaaggtg acggttcgcc tgtccgacct gaacggcccg cgcggcggac cagacaagcg
     4201 ttgccggatc cagatttcag cgcttcgttc tagcgtcgtg gtagaggacg tcgaagaaga
     4261 cctctacatc gccatcgggc gcgctgcgga ccgagccgga cgaacgctgt cgcgccgcat
     4321 cgcccggcat cgcgaacacc gcgcggtacg gatgtcccgg cccatcgaac cgcaatccgc
     4381 tcccgctact tcacatctca tgccgccggg cgaaccggct ggcgcaacgc aacattgatt
     4441 accaaggaaa ggcaaacacc atggcaaacc tgaaaacgat atccggccac tacgccggct
     4501 cggcaaccac cgactcggtc ctgtccacca acaaggtagt ccgcaacacc tatctgctgc
     4561 tgtcgatgac gctgctcttc gcagcggcga cggccggcgc atcgcttgcg ctcaatctcc
     4621 cgcatccggg cctgatcatt acgctggtgg gttacttcgg cctgctcttc ctcacctcgt
     4681 atctcaagga cagtgcatgg ggcatcgcct cggtctttgc gctcaccggc ttcatgggct
     4741 acacgctggg acccatcgtc aatcactacc tcggcctcac caacggtggg caaacggtga
     4801 tgatggcaat ggcggcgacc gcgacgatct tcatcgggct atcggcctac gccctgacga
     4861 cccgcaagga cttcaactac cttggcggat tcctgatggt cggcatcatc gtcgccttcg
     4921 tcgccggcct cgccgcgatc ttcttcgaga tcccggccct gtcgctgacg gtatccgccg
     4981 ccttcgtgct gctgatggcc ggtctgatcc tctatcagac ctcgagcatc atcaacggcg
     5041 gtgaaacgaa ctacatcatg gcgacggtca cgctgttcgt ctcgatcttc aacctgttca
     5101 cgagcctgct gcacctgctg ggattcatga actctaacga ctaaagatgg ggcgtcgcgg
     5161 caaagaggtc gcgtggccga tcgatgtcgc agacgacacc ggggtcgttg acctcgacga
     5221 cgaccccggc atctccggcc gcgtcgacca gcgcgcgcgc cgcagtcgcc agccagcgcg
     5281 gcgagttggt cgccccatgc tgcggagaac ccgaccgggt gccccgcccg atcccggaat
     5341 atcggtcggg caaccgaagc accattcgcg agggcgctca aaacaaggcg gtaactcgca
     5401 ggcgcgatga atggcatgtc tgccagggcg atcagccagc cgctattcga gcgcgtcgcg
     5461 cgcacggcaa gcgccagtga gtcgccgata ccggcggccg aattgccatt caacaggact
     5521 tcgcatcctg cttcctcgca cgccaaggca agctcatcat caccattgcg caggacgacg
     5581 atgactcgat cgcaggcggc gcgcaaagca gcagccgagc gcaccgccat cggccggccg
     5641 tctgcgagcg gctgccagtc gcggcgttcc cgcaccgtaa aggccagccg gcccttcgcc
     5701 gccaacccgt cacaaagccg taacaagggc ctcccatact ccgcctcgtt ccgatcacac
     5761 cacgggagaa cccaagtgaa tctcaataaa ctgtctctgg ccgtcgccgc gctactcgcc
     5821 ggcgtttccg ctgcccacct cgcacatgcc gccgacccca tcgtcaccat cgacggttcg
     5881 tcgaccgttt atccgatcac cgaggcggtt gctgaggaat tccagatctc gaaacggaac
     5941 aaggtccgcg tcaccgtcgg catttccggc accggcggcg gattcaaaaa actctgccgt
     6001 ggcgaggtcg acatctccgg cgcctcgcgt ccgatcaaga agtccgaaat ggaagcctgt
     6061 aaggctgccg gcattagcta ctacgaaatg ccggtcgcct tcgacgcgct gaccgtaatg
     6121 atccacccgt cgaacaagtt cgccgagacg atgaccatgg cggaactcaa gaaggcctgg
     6181 gcaccagaag ccaagggaac cgtcaccacc tggaaacaga tcaatccggc gtggcccgac
     6241 acccccatga agctctacgg ccccggtacc gattcgggca cctttgacta ctttaccgag
     6301 gcgatcaacg gcaagggcgg cgccagccgg ccggactaca cggcatcgga agacgacaac
     6361 gtgctggttc agggtatctc gcaggacaag ggcgcgctcg gttacttcgg cttctcgtac
     6421 tactgggaga accgtaacaa ggtgaaggcg gtcaaggtcg acggcggcaa gggcccggtc
     6481 gcgccgagcc ccgagtcagt ggagaacggc gcgtacgccc cgctgtcgcg cccggtcttc
     6541 atctacgtga gcgcgaaggc cctcgagaag cctgaagtca aggagttcgt cgagttctac
     6601 atggccaacg cgacgacgct ggtcaaggag gtcaagtacg tgccgttgcc ggcggcgatc
     6661 tacaagtcga acctcgacca cgtcgcaaag aagaaggttg gcaccgtctt cggtggcgag
     6721 tccgacgttg ggaccaggat cgaggacctc gtcaagcgcg aagcaaagct ctgaccacga
     6781 cctgcgcggt gacatggccc ggcactcgtc gggccatttt tttggccact accaccagat
     6841 ttccggatag cgccttgact tcggcaggtt gttcacgacc aacgcaacca gcagcagaat
     6901 tgacgccccg agcgcgactg ggatcgccgc gaagaaaaag ccaagttgct cgatatcgga
     6961 tgttccgagc gccgctatca acgcagtggc accacccggc ggatgcagcg tccgcgtcaa
     7021 atgcatgacc gcgatggccg tggcgaccgc caacgcctgg gccaacccag gcatctcgcc
     7081 cgccagcttc cagcacgcga caccgatcag ggccgaaatc acatggccgc cgaccaggtt
     7141 gcgcggctgc gccaagggac tgcgaggtgc cccatagagc agcactgccg aggcaccgaa
     7201 ggatccgatc atcaaagacg tcccgatcgt atcgagcaac ccgctagata cccaggcgac
     7261 ggcgccgaca ccgaggaagg caccgatcca ggaccaacca atctccgagt tgctgacgcg
     7321 gggcggtcca cctgcggtcg ttcctcgcca cttgcgccag tagccaacga cggcccaacg
     7381 gccactcaac
//
LOCUS       DATDAA010000018         4845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_315743,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000018 DATDAA010000000
VERSION     DATDAA010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315743"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /gene="ppk1"
                     /locus_tag="VIS73_00655"
     CDS             complement(<1..1560)
                     /gene="ppk1"
                     /locus_tag="VIS73_00655"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY5761694.1"
                     /translation="MNDVRSVPPAPTASTPLKAEDRYFDRELSLLAFNRRVLAQAQNT
                     ETPLLERLRFLCIVSSNLDEFFEIRVAGLKEQIRAGTSAKTLFGFSATGVFERVAREA
                     HDIVATQYRLLNDDILPQLETHGIQFLRRVNWTDAQTEWIRDYFRREVMPVVTPIGLD
                     PSHPFPRVLNKSLNFAIELEGKDAFGRNSGAAIVQAPRVLPRVIRLPSDIAGCDYGFV
                     FLSSIVHAFVGELFSGMAVRGVYQFRATRNSDLFVDEEEVKNLRAKLQGELPQRHFGD
                     AVRLEVADNCSPEMASFLLNQFGLAENDLYRVDGPVNLVRLMQIPDAVDLPALKFSPF
                     IPGLPKALDKPSRTIFDLIRKGDILLHHPYQSFAPVINLLRSTVEDPQVVAIKMTVYR
                     TGTDSVLMESLIRAAQNGKEVTVIVELMARFDEEANIGWATKLENVGAHVVYGVVGYK
                     THAKALMIVRREEGPNGQSILRRYIHLGTGNYHPRTARLYTDYGLLTANEEIGADVNE
                     VFKQLTGLGRAQ"
     gene            1803..3329
                     /gene="ppx"
                     /locus_tag="VIS73_00660"
     CDS             1803..3329
                     /gene="ppx"
                     /locus_tag="VIS73_00660"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEY5761695.1"
                     /translation="MKYEQVAAVDLGSNSFRLQVGRVVDGQVYPLDSVKETVRLASGL
                     QADKTLDQASIERALAALSRFGERLRGFSPEGVRAVATNTLRVAKNAPSFLRQAEAVL
                     GFPVEVIAGREEARLIFLGAAHSLPAAAHKRLVVDIGGGSTEFIIGKRFEPQLLESLY
                     MGCVSFSNRFFPGGAIDKRSFREAEIEAAAEIEQIVGHYRAHGWKEAVGSSGSAAAIS
                     ELLEQNGFNPGGVAGITGAGLLRLKTQLIKAGSIDALTLAGLRADRAPVLPGGLAIML
                     AVFDRFGLERMTYADGALRLGVLYDLIGRFHHEDRREATIAEFQRRYQVDIAQADRVE
                     SLALALYRQLRQEPGVEHEHDAQFLSWAGRLHELGISVAHSAHHKHGAYILTYADMPG
                     FSKTDQSRLALLVLGHRGKLDKVAVLAGIDANWHLLFCLRLAVLFHRSRSEYEVPRLQ
                     IASTGNGFILEVAAAWLKDNPLTAAAIDDECAQWARVGIPLTLKESREPRARIAARAG
                     "
     gene            3326..4435
                     /locus_tag="VIS73_00665"
     CDS             3326..4435
                     /locus_tag="VIS73_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5761696.1"
                     /translation="MTPAALRIDQDGATAVLSGEWTLAGLPRPLSGFDAEIARTARVA
                     MAWNLTEVVRLDSAGALLLWRQWGGRLPERIEYGEHRRTIDRIATLPAERVEEPTAQK
                     WAVLAALGHGVLWLTHNFVGVLALTGQLVIDLQHVLRRPSMMPWREFSATLYRAGAQA
                     LPVTALVGLLIGVVLSYLSALQLRNFGADAFIVDILGIGIIREFAPVLVAILVAGRSG
                     SAITAQLGAMRVTEEIDALAALGISRSLRLVLPRVLALGVAVPLLVVWTSIAGLIGGM
                     VSAQLQIGLDMGFFVETLARVVPAANLWLAGAKGIVFGLSIALVACHFGRQVRPNTNS
                     LSINTTASVVVSITTVIVLDALFAIFARGIGNPIG"
     gene            4436..>4845
                     /locus_tag="VIS73_00670"
     CDS             4436..>4845
                     /locus_tag="VIS73_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5761697.1"
                     /translation="MTATPPVIAIRELWNRLGGRDIHSGVTLDVGRGEALALTGSSGS
                     GKTTLLRSIIGLQVPHRGDIEIFGESLLAATGDARRLLHQRMGMLFQQGALFSALSVF
                     DNVAFPLRELRELDESLIRDLVLLKLHQVGLTAA"
BASE COUNT          794 a         1517 c         1637 g          897 t
ORIGIN      
        1 ctgcgcgcgt ccgagtccgg tgagttgctt gaagacctca ttgacgtcgg cgccgatctc
       61 ttcgttggcg gtgagcaggc cgtagtcggt gtacagccgc gccgtgcgcg gatggtagtt
      121 gccggtgcca agatgaatat agcgacgcag gatgctctgg ccgttcgggc cttcctcgcg
      181 gcggacgatc atcagcgcct tggcgtgggt cttgtagcca accacgccgt agacgacatg
      241 ggcgccgacg ttctccagct tggtggccca gccgatgttg gcctcctcgt cgaagcgtgc
      301 catcagctcg acgatgaccg tgacttcctt gccgttctgc gcggcgcgga tcagcgactc
      361 catcagcacc gagtcggttc cggtgcggta gacggtcatt ttgattgcaa ccacctgcgg
      421 gtcctcgacc gtcgagcgca gcaggttgat caccggcgcg aaggactggt aggggtggtg
      481 gagcaggatg tcgcccttcc ggatcaggtc gaagatggtc cggctcggtt tgtcgagggc
      541 cttgggcagc cccggaatga aggggctgaa cttgagtgcc ggcaggtcga cggcgtcggg
      601 gatctgcatc aggcggacga ggttgaccgg gccgtcgact cggtagagat cgttctcggc
      661 gaggccaaat tggttgagca ggaagctcgc catctccggc gagcagttgt cggcgacctc
      721 gaggcggacc gcgtcgccaa agtggcgctg tggcagttca ccctgcagct tggcgcggag
      781 gttcttgact tcttcctcgt cgacgaacag atcgctgttg cgggtggcgc gaaactggta
      841 gacgccgcgc acggccatgc ccgagaacaa ctcgccgacg aaggcgtgga cgatcgacga
      901 gaggaagaca aagccgtagt cgcagccggc gatgtcggat ggcagccgga tcacgcgcgg
      961 tagcacgcgt ggcgcctgga cgatcgccgc gcccgagttg cggccgaagg cgtccttgcc
     1021 ttcgagttcg atcgcgaagt tcagactctt gttgagcacg cgcggaaacg gatgcgaggg
     1081 gtcgagcccg atcggcgtca ccaccggcat cacctcgcgg cggaagtaat cgcgaatcca
     1141 ctcggtctgc gcgtcggtcc agttcacgcg ccggaggaac tggatgccgt gcgtttcgag
     1201 ctgcggcagg atgtcgtcgt tgagcagtcg gtactgggtg gcgacgatgt cgtgtgcttc
     1261 acgcgcaacg cgctcgaaga cgccggtggc gctgaagccg aacagcgtct tcgccgaggt
     1321 gccggcgcgg atttgctcct tgaggccggc gacgcggatt tcaaaaaatt cgtcaaggtt
     1381 gctcgagacg atgcagagga agcgcaggcg ctcgagcagc ggcgtctcgg tgttctgggc
     1441 ctgtgcgagg actcggcggt taaaggcgag cagcgacagc tcgcgatcga aatagcgatc
     1501 ctcggccttg agaggcgtgc tggcggtagg ggccggcggc acgctgcgga catcgttcat
     1561 gggactcact ccagccattg ggctttcacg acccgatcat acgccgaacc gacatcaata
     1621 acacgctgca aggtcatgat cgaaaaggaa attgattcgc ttttccagtg tcggctgcct
     1681 ggcgcggcgc aacaaacggc gaaatcgcgg gtgctagaat cgccgcggtc cagcattccc
     1741 ctacagcgcc cccgcgttcc caccgtgatg acgacaattt cgcaccggaa agccgcacac
     1801 gcatgaagta cgaacaggtc gctgccgtcg atctcgggtc caacagcttc cgcctgcagg
     1861 tcgggcgcgt tgtcgacggg caggtctatc cgctcgattc ggtcaaggaa accgtccggc
     1921 tcgcgtccgg gctgcaggcc gacaagaccc tcgatcaggc ttcgatcgag cgtgcattgg
     1981 cggcgctgtc gcgtttcggc gagcggctgc gcggcttcag ccccgagggc gttcgcgcgg
     2041 ttgccaccaa cacgctacgc gtggccaaga acgcgcccag cttcctgcgt caggccgaag
     2101 cggtgctggg ttttccggtc gaggtcatcg ccggccgcga ggaagcgcgc ctgatctttc
     2161 tgggtgccgc gcactcgctg ccagccgctg cccacaaacg tctggttgtc gacatcggcg
     2221 gtggatcgac cgaattcatc atcggcaagc gcttcgagcc gcaattgctc gaatcgctgt
     2281 acatgggctg cgtcagcttc tcgaaccgct tctttccggg tggcgccatc gacaagcgct
     2341 cgttccgcga agccgagatc gaggccgccg ccgaaatcga gcagatcgtc ggccactacc
     2401 gggcacatgg ctggaaggaa gcggtcggtt cctcgggcag cgccgcggca atcagcgaac
     2461 tgcttgagca gaacggcttc aatccgggcg gggtcgcggg cattaccggc gccggactgc
     2521 tccgcttgaa gacccagctg atcaaggccg gcagcatcga cgcgctgaca cttgccggcc
     2581 tgcgcgccga tcgcgcgccg gtgctgccgg gcggcttggc gatcatgctc gcggtgttcg
     2641 atcgtttcgg cctcgaacgg atgacctacg ccgatggcgc cttgcgcctc ggcgtgctct
     2701 acgatctgat cgggcgtttc caccatgaag atcggcgcga agcgacgatc gccgaattcc
     2761 agcgccgcta tcaggtagac attgcccaag cagatcgagt cgaatcgctg gcgctcgcgc
     2821 tctatcgcca gttacgccag gaacccggcg tcgagcacga gcacgacgcg cagtttctga
     2881 gctgggcggg acgcctgcat gaactgggga tttcggtggc tcacagcgcg caccacaagc
     2941 acggcgccta catcctcacc tacgccgaca tgcccggatt ctcgaagacc gaccagagcc
     3001 ggctggcact gctcgtgctc gggcatcgcg gcaagctcga caaggtcgcc gtgctggccg
     3061 gcatcgacgc caactggcac ctgctgttct gcctgcggct cgccgtgctc tttcatcgtt
     3121 cgcgcagcga atacgaagtg ccgcgcctgc agatcgccag taccggaaac ggcttcatcc
     3181 tcgaagtggc tgcagcgtgg ctcaaggaca acccactgac tgcggctgcc atcgacgatg
     3241 aatgcgcgca gtgggcgcgc gttggcattc cgttgacgct caaggaatcg cgcgagccgc
     3301 gcgcgcgaat cgccgcccgc gccggatgac accggcggcg ctgcgcatcg atcaggacgg
     3361 tgcgacggcc gtcctctcgg gcgaatggac actggccgga ttgccgcggc ccctgagcgg
     3421 cttcgatgcc gagatcgcgc gcacggcgcg agtcgccatg gcctggaacc tcactgaagt
     3481 tgtccgtctc gatagcgccg gcgcactgct gctctggcgg cagtggggcg gccgtctgcc
     3541 cgagcggatc gagtacggcg aacaccggcg gaccattgat cggattgcca cgctgcccgc
     3601 ggagcgcgtc gaggaaccga cagcgcagaa gtgggcggtg ctggctgcgc tggggcacgg
     3661 cgtgctctgg ctgacgcata actttgtcgg cgtactggcg ctgaccgggc agttggtaat
     3721 cgacctgcag cacgtgctgc ggcggccatc gatgatgccc tggcgggagt tctcggcaac
     3781 gctctaccgc gccggcgcgc aggctctgcc ggtaacggcg ctggtcggcc tgctgatcgg
     3841 tgtcgtgttg agctatctgt cggccttgca gctgcgcaat ttcggcgccg acgccttcat
     3901 cgtcgacatc ctcggcatcg gcatcatccg cgagttcgcg ccggtgctcg tggcgatact
     3961 cgtcgccggc cgctcgggtt cggcgatcac cgcccagctg ggggcgatgc gtgtcactga
     4021 ggagatcgat gcgctggcgg cgctgggcat ctcgcgcagc ctgcggctgg tcctgccgcg
     4081 cgtgctggcg ctcggtgttg ccgtgccgct gctagttgtg tggacctcga tcgccggcct
     4141 gatcggcgga atggtttcgg cgcagttgca gatcgggctg gatatgggct ttttcgtcga
     4201 aacgctggcg cgtgtcgttc ccgcggctaa cctctggcta gccggagcca agggcatcgt
     4261 cttcggcctg tcgattgcgc tggttgcctg ccacttcggc cggcaggtac ggccgaatac
     4321 caacagcctg tcgatcaaca ccacagcctc ggtggtcgtg tctatcacca cggtcatcgt
     4381 gctcgacgcg ctgttcgcga tcttcgcgcg cggcatcggc aatccgattg gctagatgac
     4441 agcgacaccg ccagtcatcg ccattcgtga actgtggaac cggctgggcg gccgcgacat
     4501 ccacagcggc gtcacgctcg atgtcgggcg cggcgaggcg ctggcgttga ccggcagttc
     4561 gggcagcggc aagaccacgc tgctgcgctc gatcatcggc ctgcaggtgc cgcaccgcgg
     4621 tgacatagag atattcggtg aatcgctgct ggcagcgaca ggcgacgcgc ggcggctatt
     4681 gcaccagcga atgggcatgc tgttccagca gggcgcgctg ttttcggcgc tgtcggtttt
     4741 cgacaacgtc gccttcccac tgcgtgagct gcgcgaactc gatgagtcgc tgattcgcga
     4801 tctggtcttg ctcaagctcc atcaggtcgg tctgacggcc gccga
//
LOCUS       DATDAA010000019        11387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_280868,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000019 DATDAA010000000
VERSION     DATDAA010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11387
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280868"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VIS73_00675"
     CDS             <1..1263
                     /locus_tag="VIS73_00675"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HEY5761698.1"
                     /translation="VGVANQRETTVLWERASGRPLAPAIVWQDRRTATICAGLREAGH
                     EAEVRRRTGLLIDPYFSATKIAWLLDAIPGARARAEAGELAFGTIDCWLAWQLTGGAL
                     HVTDASNASRTMLFNLEGGDWDADLCRTLGVPHAVLPRIVDSSGVLGEISAIPELAGV
                     PLAALVGDQQGALAGQACFAPGLAKNTYGTGCFLLVHAGEHAPQAPDGLLTTVAERRG
                     GRLNYTVEGSVFVAGALVQWLRDQLGIIDRAADIEALAASVPDTGGVALVPAFAGLGA
                     PWWDADARGLICGLTRGTTRAHIARAALEAIALQVVDLIDSMSAAGLAIDELRVDGGA
                     AANNLLLQMQADLLQRPVLRPQCLETTALGAAYLAGLAVGVWGSTDDIAQHWTLDRRF
                     EPQGSPAQAETMRARWRDAVARTRSRHK"
     gene            complement(1276..2391)
                     /gene="zapE"
                     /locus_tag="VIS73_00680"
     CDS             complement(1276..2391)
                     /gene="zapE"
                     /locus_tag="VIS73_00680"
                     /inference="COORDINATES: protein motif:HMM:NF015902.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEY5761699.1"
                     /translation="MQRNARISDQAVITGFRSALARRHVVPDEQQLVIAARLQRLFDE
                     LLAFKRARRSQLRKIFIRPPLPRGLYLYGGVGRGKSMLMNCFFDTLPFQRKRRVHFHA
                     FMREVHERLNALRSENDPLLRVADEIAAQTRLLCFDEFHVSDIADAMILGRMLDALFE
                     RGVVIVLTTNYEPDGLYPNGLQRQNFLPTIEQIKRKLDVLELDSGVDYRLRALEQRPV
                     FLVPANAEADRTLADRFVELAGGEGEMTPLVVFDRPIPVRRRALGVIWFDFDALCGGP
                     RSQNDYLEIAQNHHSVLLSGIPAMTPAQASEARRLTWLVDIFYDHRVKLIATAAAEPD
                     DLYTQGTQAGEFSRTASRLIEMRSHEYLAAQHGALAI"
     gene            complement(2397..4946)
                     /locus_tag="VIS73_00685"
     CDS             complement(2397..4946)
                     /locus_tag="VIS73_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5761700.1"
                     /translation="MKPKTIRGLLMTAAVVFSLALFTLIVLLVSVLHERAVRDEAARN
                     SDVLAQVTFNAMFQLMSTGWNRAQLESFTQAISKAVADSPTTITIYRGKPIEALFGAI
                     PGKQADATVEAVLAGAGVTRSEVGDEVRSVFPVVAKEVCQRCHYNVQVGTVMGAIDVH
                     QQVSGAIGEARRNLIIATLPVVPLALVATLLMAAYINRRITGSIDLLAEDIQAVTRVA
                     DLRRLGARSGDFGFAEFGAISHEIRQLTEKLRSIAVDKSMLEFEIRLLEKFVITSEVV
                     RDWREYVNHLLIEINDVMEAYALFSIFKVDDELFDLEVFWYRQPAESVRVRFEAALHD
                     ALSTNPYFGDGYAVNIVHNVADRSGRIDELPEESLKVQTKSLMVAAPKIGGIVGIGVQ
                     AQSLDDASRELVLESILSTLLNVVGSVKAIHKYTKELEYYATRDPLTNLYNQRVFWEL
                     LDNELARSERHGSRFALVVIDLDNFKSVNDTFGHAVGDIFLQQVSAVMREALRGDDLL
                     ARYGGDEFVAILPEADLGNASEVAARINRNVEAMTVDTADGAHVRGSVSIGLAVYPDH
                     ARETRDLFLFADSMMYRAKADGKNRVAIPTEQDVVEVFRSIGEKSQYILQAVEDRRVE
                     PYFQPIVEMGSGRIAAVEVLGRLRVGDDRLLVGDDFVPLAEKMGVMHRLDYILMEKAL
                     AAVAEAGFAGDIFLNMSPRALVLNDFIAQVGRIASDSGIAPERIVFEITERETIKNMA
                     VLEKFIASLKQDGFKLAIDDFGSGFSSFHYVKRFPIDILKIEGDFVLNMVNSDKDRAL
                     VRSIVALAKELGIRTVAEYVETEAVFDAVQELGIDLGQGHHIGRPRAAVGV"
     gene            complement(5090..6511)
                     /gene="lpdA"
                     /locus_tag="VIS73_00690"
     CDS             complement(5090..6511)
                     /gene="lpdA"
                     /locus_tag="VIS73_00690"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY5761701.1"
                     /translation="MPKQFDVLVIGGGPGGYVAAIRAAQLGFVTACCESAPYADPKGE
                     PRLGGTCLNVGCIPSKALLRSSELFEEAGHSFDMHGISVGTPKLDVAKMVGRKNTIVN
                     QLTQGIKGLFKKNKVTFLAGHGKFVGQGQGWQIDVGGEIVEAKHVIIATGSKPRHLPG
                     IPVDNKIVCDNVGALDFDAVPKKLGVIGAGVIGLEMGSVWRRLGSEVTVLEGMPDFLA
                     FADHDIAKEALKLFTKQGLDIRMGVKIGTTTVGKKGVALEYTDKDGKAQKLECDRLIV
                     SVGRVPNTDGLDADKVGLKINDRGQIDVDDHCQTNLPNVWAIGDVVRGPMLAHKASEE
                     GIMVAELIAGQAGHCNMDTIPGVIYTHPEIAWVGKTEKQLKDAGIAYKVGKVPFMASG
                     RALGHGDASGFVKMLACAQTDRILGVHVIGVNASELISEGVVAMEFHGASEDLARICH
                     AHPTLSEIMHEAALACDKRAIHF"
     gene            complement(6604..7800)
                     /gene="odhB"
                     /locus_tag="VIS73_00695"
     CDS             complement(6604..7800)
                     /gene="odhB"
                     /locus_tag="VIS73_00695"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005400412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEY5761702.1"
                     /translation="MIIDVKVPQLSESVAEATLVAWHKREGQAVSRDENLIDIETDKV
                     VLELPAPDGGVLVKIIKGDGETVVAGEVIAQIDTAATATASAPAAAAAPAAAPAAAAA
                     PAAKSAPAGAASPAASKILAEKGVAAGDVAGSGRGGRVTKEDAVKAGAPAVRAAAPTV
                     SLPQGDRIEERVPMSRLRARIAERLLQSQAQNAILTTFNEVNMAPVMALRKQYGEKFE
                     KEHGVRLGFMGFFVKAACAALKKFPIINASVDGSDIVYHGYIDIGIAVGSPRGLVVPI
                     IRDADQLSIAEIEKKIAEFGQKAKDGKLGIEELTGGTFSISNGGIFGSMMSTPIINPP
                     QSAILGIHATKDRAVVENGQVVVRPMNYLAMSYDHRIIDGREAVLGLVTMKEALEDPA
                     RLLLGV"
     gene            complement(7819..10635)
                     /locus_tag="VIS73_00700"
     CDS             complement(7819..10635)
                     /locus_tag="VIS73_00700"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY5761703.1"
                     /translation="MMQQHSSNSLLFGGNAPFVEELYEAWLDNPQSVPEEWRAYFDKL
                     AQLPGNAARDVPHAPVIAAFADMAKQSVRPIVVASEHKKQVAVLQIINAYRFLGTRVA
                     DLDPLKRTERPAIAELDPAFYGFTDADMNARYNVGSYGAVGAEEATLKQVLDSLKETY
                     CGSIGVEYMYMSNINEKRWLQGKFEPIRGKGTYSADDRKRFLERLTAAETLERYLHTK
                     YVGQKRFSLEGGEALILSMDELIRVSGGLGVQELVIGMAHRGRLNVLVNTLGKQPSML
                     FAEFEGKKAQDLSAGDVKYHMGYSSDVGTPGGPVHLTLAFNPSHLEIVNPVVEGSVYA
                     RQIRRGENGKAEVLPVLIHGDSAVAGQGVNQEMLNFSQTRGYGTGGTVHIVVNNQIGF
                     TTSDPRDYRSSLYCTDIFKMVEAPIFHVNGDDPEAVALATKIAMEFRQEFRKDVVIDI
                     ICFRKLGHNEQDEPMVTQPLMYRKVSAHPGTRKLYADRLVAQNVLTAEGPDQMIKDYR
                     AALDEGRHLVDPVLTDYQSKYAIDWTPYIGHPYTEVCDTTVPIKELQRLATRLTTTPE
                     GFALHSRVKKIIDDRAAMGEGKIPVDWGMAENLAYATLLAAGYGVRISGEDVGRGTFF
                     HRHAALHDQNRERWDEGTYHPLANLQPDQGRFQCYDSVLSEEAVLAFDYGYATATPNE
                     LVVWEGQFGDFANGAQVVIDQFLASGESKWGRGCGLVMLLPHGYEGQGPEHSSARLER
                     YLQLSAEFNWEVIMPSNAAQIYHALRRQMLRKHRKPLIVMSPKSLLRHKDAGCSLEDL
                     AKGTFHTVVGETEKLDAKKVTRVVACAGKIYHELAAARAERKITNVAIVRMAQLYPFD
                     DRRLGEELAKYPNLKDLVWCQEEPENQGAWYAKHHRLAALVKKGQTLSCVSRPASASP
                     AVGYAAKHAVQQKDVIDRALGDAK"
     gene            complement(10733..>11387)
                     /locus_tag="VIS73_00705"
     CDS             complement(10733..>11387)
                     /locus_tag="VIS73_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY5761704.1"
                     /translation="NPVLVRALDIIFTLHADHEQNASTSTVRLAGSSGANPFACISAG
                     IACLWGPAHGGANEACLQMLEEIGDVSRVGEYIKRAKDKSDSFKLMGFGHRVYKNFDP
                     RAKLMGKVCADVLAELGLENDRLFKLAKALEKIALEDEYFVEKKLYPNVDFYSGIVQK
                     ALGIPTSMFTCIFALARSVGWMTQWEEMITDPEYKIGRPRQLYTGAAKRDVVPLAKR"
BASE COUNT         1997 a         3596 c         3669 g         2125 t
ORIGIN      
        1 gttggcgttg ccaaccagcg cgagacgacg gtgctctggg agcgcgccag cgggcggccc
       61 ctggcgccgg cgatcgtctg gcaggaccga cgcacggcga cgatctgtgc cggtttgcgc
      121 gaggcaggcc acgaagccga ggtccggcga cgcacgggcc tgctgatcga cccctacttt
      181 tcggcgacca agatcgcctg gctgctcgac gcgattcccg gcgcacgggc ccgtgctgaa
      241 gccggcgagc tggccttcgg caccatcgac tgctggctcg cctggcaact gactggcggc
      301 gcgcttcacg tcactgatgc gagcaatgcg tcgcggacaa tgctgttcaa tctcgaaggc
      361 ggcgactggg atgccgatct ctgccggacg ctcggcgtgc cacacgccgt gctgccgcgc
      421 atcgtcgatt cgtcgggggt gctcggcgaa atcagcgcga taccggaact ggccggcgtg
      481 ccgctggccg cactggtcgg cgaccaacag ggtgcgctgg ccggacaggc ctgctttgct
      541 ccgggcctag cgaaaaatac ctatggcacg ggttgcttcc tgctcgtgca tgccggagag
      601 cacgcaccac aggcacccga cgggctgctg accaccgttg ccgagcgccg tggcgggcga
      661 ttgaactaca ccgtcgaggg cagcgtgttc gttgccggtg cattggtgca gtggctgcgc
      721 gaccagctcg gcatcatcga ccgcgccgcc gacatcgagg cgctcgctgc ctcggttccc
      781 gataccggcg gcgtggcgct ggtgccggcc ttcgccggcc ttggcgcgcc ctggtgggac
      841 gccgacgcgc gcgggctgat ctgcggcctg acccgcggca cgacccgcgc ccacatcgcc
      901 cgtgcggcgc tggaagcgat tgcgctgcag gtggtcgacc tcatcgacag catgagcgcc
      961 gccgggctgg cgatcgacga gctgcgtgtc gatggcggcg cggcggccaa caacctgctg
     1021 ctgcagatgc aggccgacct gctgcagcga ccggtgctgc gtccgcagtg cctggagacg
     1081 acggcgctcg gtgccgccta tctggcgggt ctagcggtcg gtgtctgggg ctcgaccgac
     1141 gacatcgccc agcactggac gctggacagg cggttcgagc cgcagggttc gccggcgcag
     1201 gccgagacga tgcgcgcgcg ctggcgcgac gccgtggcgc gcacgcgcag ccgacacaag
     1261 tagcagagac ctggcttaga tcgccagcgc gccgtgctgg gcagctaggt attcgtgcga
     1321 gcgcatctcg atcaagcggc tcgccgtgcg tgagaactcg ccagcctgcg ttccctgagt
     1381 gtagaggtcg tccggctcgg cggctgccgt ggcgatcagt ttgacgcggt ggtcgtagaa
     1441 gatgtcgacc agccaggtca accgtcgcgc ctcggaggcc tgcgccggcg tcatcgcggg
     1501 aattcccgag agcaacaccg agtggtggtt ctgcgcgatc tcaaggtagt cgttctgcga
     1561 ccgcggcccg ccgcagaggg catcgaaatc gaaccagata acgccgagcg cgcggcggcg
     1621 caccggaatt gggcggtcga agaccaccag cggcgtcatc tcgccttcgc caccggccag
     1681 ctcgacgaag cggtcggcta acgtccggtc ggcctcggcg ttggccggga cgaggaagac
     1741 cgggcgctgt tcaagcgccc gcagccggta atcgacaccg ctgtcgagtt caagcacgtc
     1801 aagcttgcgc ttgatctgct cgatggtcgg caggaagttc tggcgttgca ggccgtttgg
     1861 gtaaaggccg tctggttcgt agttggtggt aaggacgatc accacgccgc gctcgaacag
     1921 cgcatcgagc atgcggccaa ggatcatcgc gtcggcgatg tccgagacat ggaactcgtc
     1981 gaagcacagc agccgcgtct gcgcggcgat ttcgtcggcg acccgcagca gcggatcgtt
     2041 ctcgctccgg agtgcgttga ggcgctcgtg cacctcgcgc atgaaggcgt ggaaatgcac
     2101 gcggcgcttg cgctggaacg gcaacgtgtc gaagaagcag ttcatcagca tgctcttgcc
     2161 gcggccgaca ccgccgtaga ggtagagccc gcgcggcagc ggcggccgga tgaagatctt
     2221 gcgcagctgc gagcggcgcg cacgcttaaa ggcaagcagt tcatcgaaca gtcgctgcag
     2281 gcgcgcggca atcaccagct gctgctcgtc tggaaccaca tggcggcgcg caagcgccga
     2341 gcggaagccg gtgatgaccg cctgatcgga aatccttgcg tttcgctgca tcagactcag
     2401 accccgacgg cagcgcgcgg ccggccgatg tggtgaccct ggccgagatc gatgccgagt
     2461 tcctgcacgg cgtcgaagac ggcttcggtc tcgacgtact cggcaaccgt acggattccc
     2521 agttccttag ccagcgcgac gatgctgcga accagcgccc gatccttgtc actgttgacc
     2581 atgttgagga cgaagtcgcc ttcgatcttg aggatgtcaa tggggaagcg cttgacgtag
     2641 tggaacgatg aaaaacccga accgaagtcg tcgatcgcca gcttgaagcc gtcctgcttg
     2701 aggctggcga tgaatttctc gaggaccgcc atgttcttga tcgtttcgcg ctcggtgatc
     2761 tcgaagacga tgcgctcggg tgcgattccg ctgtcgctag cgatgcgccc cacctgcgcg
     2821 atgaaatcgt tgagtaccag cgcccgcggc gacatgttga ggaagatgtc accggcaaag
     2881 ccggcctcgg cgaccgccgc cagcgccttc tccatcagga tgtagtcgag ccggtgcatg
     2941 acgcccatct tctcggcgag cggcacgaag tcgtcgccga ccagcagtcg gtcgtcgccg
     3001 actcgcagcc ggccgagcac ctcgaccgcc gcgatccggc cgcttcccat ctcgacgatg
     3061 ggctgaaaat acggctcaac acgcctgtcc tcgaccgcct gcaggatgta ctggctcttc
     3121 tcgccaatgc tgcggaagac ctccacgaca tcctgttcgg taggaatcgc gacgcggttt
     3181 ttgccgtcgg ccttggcgcg atacatcatg ctgtcggcga agaggaacag gtcgcgagtc
     3241 tcgcgcgcgt ggtccgggta gacggcaagg ccgatcgaga cgctaccgcg cacatgggcc
     3301 ccgtcggcgg tatcgaccgt catcgcctcg acgttgcggt tgattcgcgc cgccacctcg
     3361 ctagcattgc ccaggtcggc ctcgggcagg atggcgacga attcgtctcc gccgtagcgc
     3421 gccagcagat cgtcgccacg caacgcttcg cgcatgacag ccgaaacctg ctgcaggaaa
     3481 atatcgccga cggcatggcc aaaggtgtca ttgacgctct tgaagttgtc gaggtcgatc
     3541 accaccagcg caaaacggct gccatgccgc tccgaacgcg ccagttcgtt gtcgagcagc
     3601 tcccagaaga cgcgctgatt gtagaggttg gtgagcgggt cgcgggtcgc gtagtactcg
     3661 agttccttgg tgtacttgtg aatcgccttg actgagccga cgacgttgag cagcgtcgag
     3721 agaatgctct cgagcaccag ctcgcgcgag gcatcgtcga ggctttgcgc ctgcacaccg
     3781 atccctacga tgccgccgat cttcggcgct gcgaccatca atgacttggt ctggaccttg
     3841 agcgattctt cgggcagctc gtcgatccgc cccgagcgat cggcaacgtt gtgcacgatg
     3901 ttcaccgcgt aaccgtcgcc gaaataggga ttcgtcgaca gcgcatcgtg caaagcggct
     3961 tcgaagcgga cgcgcacgga ttcggcaggc tggcggtacc agaagacctc gaggtcgaac
     4021 agctcgtcgt cgaccttgaa gatcgaaaaa agcgcgtagg cctccatcac gtcgttgatt
     4081 tcgatcagca ggtgattgac gtactcgcgc cagtcgcgaa ccacctcgga ggtgatgacg
     4141 aatttctcga gcaggcggat ttcgaactcg agcatgctct tgtcgacggc gatgctacgc
     4201 agcttctcgg ttagctggcg gatttcgtgg ctgatggcgc cgaattcggc gaatccgaaa
     4261 tcccccgagc gcgccccgag gcggcgcagg tcggcgacgc gcgtcaccgc ctgaatgtcc
     4321 tcggccagca ggtcgatcga cccggtaatg cgccggttga tataggcggc catcagcaag
     4381 gtcgcaacca gcgctagcgg caccaccggc agcgtggcga taatcaggtt gcggcgcgcc
     4441 tcgccgatcg ccccggaaac ctgctgatgg acgtcgatcg cccccatcac cgtgccgacc
     4501 tgcacgttgt aatggcagcg ctggcaaacc tccttggcga cgacgggaaa gacgctgcgc
     4561 acttcgtcgc cgacttcgct gcgtgtcacg ccagcaccgg cgaggaccgc ctccaccgtc
     4621 gcatcggcct gcttgccggg aatggcccca aacagcgcct cgatcggctt gccacggtag
     4681 atcgtgatcg tcgttggcga gtcggcgacg gccttgctaa tcgcctgcgt gaagctctca
     4741 agctgggcgc ggttccagcc cgtgctcatc agctggaaca tcgcgttaaa ggtgacctgt
     4801 gccagcacgt cgctgttgcg agccgcctcg tcgcgcaccg cgcgctcgtg gagcacggag
     4861 acgaggagca cgatcagcgt gaacagggcc agcgagaaaa ccactgccgc cgtcatcagc
     4921 aatccgcgga tggtcttcgg tttcatgagt acatcttcgt cggagttcgc cgggaggcga
     4981 ttatggcggc aatgctacgc ccgggcgcaa gacaaaagaa agggcgcatt gcgccctctc
     5041 tcacactttg ccgaaaagcg ttggctcatc aatcgacgag cccggcaact cagaagtgaa
     5101 tcgcccgctt gtcgcaggcc agcgccgcct cgtgcatgat ttccgacagc gtcgggtgcg
     5161 cgtggcaaat gcgcgcaaga tcttcggagg ctccgtggaa ctccatcgcc accacacctt
     5221 cggagattag ctccgacgca ttgacgccga tgacgtgcac gccgaggatg cggtcggtct
     5281 gcgcgcaggc cagcatcttc acgaagcccg aggcgtcgcc gtggcccagc gcccggccag
     5341 aggccatgaa cggcaccttg ccgaccttgt aggcgattcc ggcgtccttg agctgcttct
     5401 cagtcttgcc gacccaggcg atttccgggt gcgtgtagat gacgcccggg atcgtatcca
     5461 tgttgcagtg cccggcctga ccggcaatca gctctgcaac catgattcct tcttcgctgg
     5521 ccttgtgcgc cagcatcggg ccgcgaacga cgtcgccgat cgcccagacg ttgggcagat
     5581 tggtctggca gtggtcatcg acatcgatct gaccacggtc gttgatctta aggccgacct
     5641 tgtcggcgtc gaggccatcg gtattcggca cgcggccgac cgagacgatc agccggtcgc
     5701 attcgagctt ctgcgccttg ccgtccttgt cggtgtactc gagggcaacg cccttcttgc
     5761 cgacagttgt ggtgccaatc ttgaccccca tgcggatatc gaggccctgc ttggtgaaga
     5821 gcttcagcgc ttccttggcg atgtcgtgat cggcgaaggc gaggaaatcc ggcatgcctt
     5881 cgagtaccgt aacttcggag ccgaggcgac gccagaccga acccatttcg aggccgataa
     5941 cgccggcgcc gatgacgccg agcttcttcg gcacggcatc gaaatcgagc gcgccgacgt
     6001 tgtcgcagac gatcttgttg tcgaccggga tgcccggcaa gtggcgcggc ttcgagccgg
     6061 tggcgatgat gacgtgcttg gcttcgacga tctcgccgcc gacgtcgatc tgccagccct
     6121 gcccctgacc gacgaacttg ccgtggccag cgaggaaggt gaccttgttc ttcttgaaca
     6181 ggcccttgat gccctgcgtc agctggttga cgatggtgtt tttgcggccg accatcttcg
     6241 ccacgtcgag cttcggcgtg ccgaccgaga tgccgtgcat gtcgaaggaa tgccccgcct
     6301 cttcgaacag ctcggacgaa cgcaagagcg ccttcgaggg aatgcagcca acgttaagac
     6361 aggtaccgcc aaggcgcggc tcgcccttgg gatccgcgta gggcgccgac tcgcaacaag
     6421 cggtgacgaa accgagttgg gcagcacgaa ttgccgcgac gtaaccgccg ggaccgccac
     6481 cgatgaccag aacatcaaat tgcttgggca tgttcatttc cttttcttga ccacaaagac
     6541 gcagagacac ggagggccac agaggatttt ccctgtgact ccgtgcctct gtggtgaatg
     6601 gctttagacg ccgagcagaa ggcgcgccgg atcttcgagc gcttccttca tcgtcaccag
     6661 accgagcact gcctcgcgac catcgataat ccggtggtca taggacatcg ccagataatt
     6721 catcgggcga acgacgacct gtccgttctc gaccacggcg cggtccttgg tggcgtgaat
     6781 gccgaggatc gccgactgcg gcgggttgat gatcggcgtc gacatcatcg agccgaagat
     6841 gccgccgttg gagatcgaga aggtgccgcc ggtcagctcc tcgatgccga gcttgccgtc
     6901 cttggccttc tggccgaact cggcaatctt cttttcgatc tcggcgatcg acagttgatc
     6961 ggcatcgcgg atgatcggca cgaccaggcc gcgtgggctg ccgacggcaa tgccgatgtc
     7021 gatgtagccg tgatagacga tgtcactgcc gtcgaccgag gcgttgatga tcgggaattt
     7081 cttcagtgcc gcacaggcgg ccttgacgaa gaagcccatg aagcccaggc ggacgccgtg
     7141 ttccttctcg aacttctcgc cgtactgctt gcgcagcgcc atcaccggcg ccatgttcac
     7201 ttcgttgaag gtggtcagga tcgcgttctg tgcctgcgac tgcagcagac gctcggcaat
     7261 gcgggcgcgc aggcgcgaca tcggcacacg ctcctcgatg cggtcgccct gcgggaggct
     7321 gactgtcggc gccgcggcgc gcacagctgg cgcaccggcc ttgaccgcgt cttccttggt
     7381 cacacggcca ccgcgaccgc taccggcgac gtcaccagcg gcaacgccct tctcggcgag
     7441 gatcttgctg gcagccgggc tggcagcacc ggccggggcg gatttggcgg ccggcgcagc
     7501 agccgccgcg ggtgcggcag cgggcgccgc tgcggcggcc ggggcgcttg ccgtggcagt
     7561 cgccgccgtg tcgatctggg cgatcacttc accggcaacg acggtctcgc cgtcaccttt
     7621 gatgatcttg accagcacgc cgccgtcggg cgccggcagt tcgaggacga ccttgtcggt
     7681 ctcgatgtcg atcaggtttt cgtcgcgcga gacggcctgt ccctcgcgtt tgtgccaggc
     7741 caccagcgtg gcctcggcga cggactcgga aagctgcgga actttgacgt cgatgatcat
     7801 gatgggtttc ctcttctgtt atttggcatc gccgagcgcg cggtcgatga catccttttg
     7861 ctgcacggca tgcttcgcgg cgtagccgac ggcgggcgag gccgacgccg ggcgcgacac
     7921 gcaggaaagc gtctggccct tcttgaccag cgctgccagg cggtgatgct tggcgtacca
     7981 ggcgccctgg ttctccggtt cctcctgaca ccagacgaga tccttgaggt tcggatactt
     8041 ggcgagttcc tcaccgagac gacggtcatc gaacggatag agctgcgcca tgcgcacgat
     8101 cgcgacgttg gtgatcttgc gctcggcgcg cgccgcagcg agttcgtgat aaatcttgcc
     8161 ggcgcaggcg acgacgcgcg tgaccttctt cgcatcgagc ttctcggttt cgccaaccac
     8221 cgtatggaag gttcccttgg caagatcttc gagcgaacag ccggcgtcct tgtgccgcag
     8281 cagcgacttg ggcgacatca cgatcagcgg cttgcgatgt ttgcgcaaca tctgccgacg
     8341 cagcgcgtgg taaatctgcg cggcgttcga cggcatgatc acttcccagt tgaactcggc
     8401 cgagagctgc aggtagcgtt cgaggcgcgc cgagctgtgc tcggggccct ggccctcata
     8461 gccatgcggc agcagcatca ccaggccgca accacgaccc cacttcgact cacccgaggc
     8521 gaggaactgg tcgatgacga cctgcgcgcc attggcgaag tcgccgaact ggccctccca
     8581 cactaccagt tcgttcggcg tcgccgtcgc gtaaccgtaa tcgaatgcca gcaccgcctc
     8641 ttcggagagc accgagtcgt agcactggaa acggccctga tcgggctgca gattggccag
     8701 cgggtggtag gttccctcgt cccagcgctc gcgattctgg tcgtgcaggg cagcgtggcg
     8761 gtggaagaag gtaccgcgac cgacgtcttc gccggagatc cgcaccccgt agccggcggc
     8821 gagcagagtc gcgtaggcca ggttctcggc catgccccag tcgaccggga tcttgccctc
     8881 gcccatcgcc gcgcggtcgt cgatgatctt cttgacgcgc gaatgcagcg cgaagccctc
     8941 gggcgtagtg gtcagccgcg ttgctaggcg ctgcagttcc ttaatcggca ccgtggtgtc
     9001 gcaaacctcg gtgtacggat gaccaatgta tggggtccaa tcgatcgcgt atttgctctg
     9061 atagtcggta agcaccggat cgaccagatg gcgaccttcg tcgagcgccg cccggtagtc
     9121 cttgatcatc tggtccggac cctcagcggt cagcacgttc tgcgccacca gcctgtcagc
     9181 gtagagcttg cgcgtacccg gatgcgccga aaccttgcgg tacatcagcg gctgcgtgac
     9241 catcggttcg tcctgctcgt tgtggcctag cttgcggaaa cagatgatgt cgatcacgac
     9301 atcctttctg aactcctggc ggaactccat ggcgatcttg gtcgccaacg cgacggcttc
     9361 gggatcgtcg ccattgacgt ggaagatcgg cgcctcaacc attttgaaga tgtcggtgca
     9421 gtagagcgac gagcggtagt cacgcgggtc ggaggtggtg aagccgatct ggttgttgac
     9481 gacgatgtgc accgtgccgc cggtgccgta accgcgcgtc tgagagaagt tcagcatctc
     9541 ctggttcaca ccctgaccgg ctaccgccga gtcaccgtgg atcagcaccg gcaatacctc
     9601 tgccttgccg ttctcgccgc gacggatctg ccgcgcgtag acggagccct cgaccaccgg
     9661 gttgacgatc tccaggtgcg acggattgaa ggccagcgtc agatgcaccg gcccgcccgg
     9721 cgtgcctaca tccgacgaat aacccatgtg gtacttgacg tcgcccgccg agagatcctg
     9781 cgccttcttg ccttcgaact cggcgaaaag catcgacggc tgcttgccca gggtattgac
     9841 caggacgttg aggcgaccgc ggtgcgccat gccgatcacc agttcctgga cacccaggcc
     9901 accggagacg cgaatcagtt cgtccatcga caggatcagc gcttcgccgc cttcgagcga
     9961 gaaacgcttc tggccaacgt acttggtgtg caggtagcgt tcgagcgttt cggcggcagt
    10021 caggcgctcg aggaagcgct tgcggtcgtc ggccgaatag gtgcccttgc cacggatcgg
    10081 ctcgaatttg ccctgcagcc agcgcttctc gttgatgttg ctcatgtaca tgtactcaac
    10141 gccgatcgac ccgcaatagg tctccttcag cgaatcgagc acctgcttga gcgttgcttc
    10201 ttcggcaccg actgcaccgt aggaaccaac gttatagcgc gcgttcatgt cggcgtcggt
    10261 gaagccgtag aaggccggat caagttcggc gatcgccgga cgctcggtgc gcttgagcgg
    10321 atcgagatcg gcaacgcggg taccgaggaa gcggtaggcg ttgatgatct gcagcactgc
    10381 cacctgcttc ttgtgttcgc tggcaacgac gatcgggcgg acgctctgct tcgccatgtc
    10441 ggcgaaggcg gctatcaccg gcgcatgcgg gacgtcgcgc gcggcgttgc caggaagctg
    10501 cgccagcttg tcgaaatagg cacgccattc ctccgggacg gactggggat tgtcgagcca
    10561 ggcttcgtaa agttcttcga cgaaaggcgc gttgccgccg aagagaagcg agttggaaga
    10621 gtgttgctgc atcatctgtt ggtcacctga tgtgagttcg gtctctgctg aattggggtc
    10681 tgccagggaa tgacaaaaag ggcggtcacg ccgcccctct tgcatcgcct ccttagcgct
    10741 tggcgagcgg aacgacgtcg cgcttggcgg cgcctgtgta gagctgacgc ggacgcccga
    10801 tcttgtactc gggatcagtg atcatctcct cccactgggt catccagcca accgaacggg
    10861 ccagcgcaaa gatgcaggta aacatcgagg tcgggatgcc gagtgccttc tgcacgatgc
    10921 ccgagtagaa gtcgacgttc gggtagagct tcttctcgac gaagtactcg tcttcgagcg
    10981 cgatcttttc aagtgccttg gccagcttga acaggcggtc gttttccaga cccagttcgg
    11041 caagcacgtc ggcgcagacc ttgcccatca acttggcacg cgggtcgaag ttcttgtaga
    11101 cgcgatggcc gaagcccatc agcttgaacg aatcgctctt atccttggcg cgcttgatgt
    11161 actcaccgac gcgcgatacg tcgccgatct cttcgagcat ctgcaggcag gcctcgttgg
    11221 cgccgccgtg tgccggaccc cagaggcagg cgatgccagc cgagatgcag gcaaacgggt
    11281 tggcacccga tgagcctgcg agacgaaccg tcgaggttga ggcgttctgc tcgtgatcgg
    11341 catgcagcgt gaaaatgatg tcgagcgcac ggaccagaac ggggttc
//
LOCUS       DATDAA010000020         2507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_176039,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000020 DATDAA010000000
VERSION     DATDAA010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176039"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VIS73_00710"
     CDS             complement(<1..305)
                     /locus_tag="VIS73_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY5761705.1"
                     /translation="MKAARIPALLLCALAWLLCACRPPAEPAAAEPPEIRVVTSGGFT
                     AAYNILAPQFEEKTGVRLVTEYGASSGGAPDSIPERLARGERFDLVILSRSSLDRLA"
     gene            complement(557..2470)
                     /locus_tag="VIS73_00715"
     CDS             complement(557..2470)
                     /locus_tag="VIS73_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY5761706.1"
                     /translation="MKHCTARAGRRPALTLSVSGMASVLLTMCLVSPSFADDFVVERN
                     VMIPMRDGVLLAMDVYRPAVDDSAVAEPLPLVLTRTPYGKDRESTVATAEHFARHGYV
                     AVIQDMRGRYESQGEFSKYSVLEPADGYDTVEWLAKQAYSNGRIGMWGTSYGAHTQAD
                     AAKLDPASLRAMLLNQGGMANAWDHAVRHGGAFELGRELTWAFRQIPEESDDPLVRAL
                     FEKEEITAWYSALPIRPGLSPLSVAPEYEAYILNEYTRSDYGDFWRRISLNWSEHYED
                     TADAAMLHVGGWYDIFLRGTIRNYVELSRLKNSPVELMIGPWTHSGNDEAFAGDVDFG
                     RAAAIADFDTDFQRRWFDRSLKNPAAAPPENPVRLFVMGTGDGHRTEEGRLYHGGYWT
                     EAPAWPLPDAETAPFYFHADGSLTREPPNARRSSTTYTFDPSHPVPTLGGNVSARVKD
                     GAFDQRERPDFHGSRPPFLPLRSRSDVLVFQTAPLEDDVVVIGPIEVVLFASSTAVDT
                     DFTAKLVDVYPPGEDFPGGFDMNVSDALVRASYRDDRHTRDLIEPGRVYRLIIRPFAT
                     ANVFKKGHRIRVDISSSNFPRFDVNPNTGEPLGQHRRTVSADNTIYHERENASHILLP
                     ILPAPPRRSGFNE"
BASE COUNT          409 a          821 c          868 g          409 t
ORIGIN      
        1 tccgccaggc gatcgagcga cgacctcgac aggatgacca ggtcgaagcg ctcgccgcgc
       61 gcgaggcgct ccggtatcga atccggcgcg ccgccggagg atgcgccgta ttcggtgacg
      121 agccggacgc cggtcttctc ctcgaactgc ggagcgagaa tgttgtaagc cgcggtgaag
      181 ccgccggagg tgacgacgcg aatttccggc ggttctgcgg ccgcgggctc cgccggcggc
      241 cggcaggcgc aaaggagcca tgcgagcgcg cagagcagta aggccgggat tcgggcagcc
      301 ttcacccgga atccccggac tcttcgagaa atcgccggcc tgcctcggtc gccgaaacga
      361 gcctgccacg gcccgacggg ccatatccga cgctcacgag accggcgtcc accgcgtctt
      421 cccacaccgt gaggcggggg caggaagtgc gccaggcctc catcacctcg tcgtaagcgc
      481 gcggctgttc gcagagccag cgcacgaggt cgcggacgag tgacgcgttg tcgagccttg
      541 cttccgccaa cgggcgctac tcgttgaacc cgcttcggcg cggcggcgcc ggcaggatcg
      601 gcagcaggat gtgcgacgcg ttttcgcgct cgtggtagat ggtgttgtcg gcgctgaccg
      661 tgcgccggtg ctgccccagc ggttcgccgg tattggggtt gacgtcgaaa cggggaaaat
      721 tgctgctcga gatgtcgacg cgaatgcggt ggcccttctt gaatacgttc gcggtcgcaa
      781 acggccggat gatcagccgg tagacccggc cgggctcgat caggtcgcgc gtgtgcctgt
      841 cgtcacggta gctcgcgcga acgagcgcgt ccgagacgtt catgtcgaaa ccgccgggga
      901 agtcctcgcc gggcgggtag acgtctacga gcttggccgt gaagtccgtg tcgacggcgg
      961 tggacgacgc aaacagcacg acctcgatcg ggccgatcac gaccacgtcg tcttcgagcg
     1021 gcgcggtctg gaacaccagc acgtcgctcc tggacctgag cggcaggaac ggcggccggg
     1081 acccgtgaaa gtccggccgc tcgcgctggt cgaaggcgcc gtccttgacg cgcgccgaga
     1141 cgttgccgcc gagcgtcggc accgggtgcg acggatcgaa cgtgtacgtc gtcgacgaac
     1201 gcctggcgtt gggcggctcg cgagtcagcg aaccgtcggc gtggaagtag aagggcgcgg
     1261 tctcggcgtc gggcagcggc cacgccggcg cctcggtcca gtagccgccg tgataaagac
     1321 gaccttcttc ggttcgatga ccgtcgccgg tgcccattac gaacaggcgc accgggttct
     1381 ccgggggagc cgcggccggg ttcttgagcg accggtcgaa ccagcggcgc tggaagtcgg
     1441 tgtcgaagtc ggcgatcgcc gccgcccgcc cgaagtcgac gtcgccggca aaggcctcgt
     1501 cgttccccga gtgcgtccag gggccgatca tcagctcgac cggcgagttc ttgagccggc
     1561 tcagctcgac gtagttccgg atcgtgccgc gcaggaagat gtcgtaccag ccgccgacgt
     1621 ggagcatcgc cgcatcggcc gtgtcttcgt agtgctcgga ccagttgagt gaaatgcgcc
     1681 gccagaagtc gccgtagtcg gagcgcgtgt actcattgag aatgtaggct tcgtactcgg
     1741 gcgcgaccga cagcgggctc aggcccgggc gaatcggcag cgcgctgtac caggcggtga
     1801 tctcctcttt ctcgaacagg gcgcgcacga gcgggtcgtc cgattcctcg gggatctgtc
     1861 tgaacgccca ggtcagctcc cgcccgagtt cgaacgcgcc gccgtggcgg accgcgtggt
     1921 cccaggcgtt cgccatgccg ccctggttca ggagcatcgc ccggagcgac gccggatcga
     1981 gcttggcggc atccgcctgc gtatgcgcgc cgtaggacgt gccccacatg ccgatgcggc
     2041 cgttcgagta agcctgcttc gcgagccatt ccaccgtgtc gtaaccgtcc gcgggttcca
     2101 gcaccgagta tttcgagaac tcgccctgcg attcgtagcg gccgcgcatg tcctggatca
     2161 ccgcgacgta accgtgacgg gcgaaatgtt cggccgttgc aaccgtgctt tcccggtcct
     2221 ttccgtaagg cgtgcgcgtc aggaccagcg gcaaaggttc cgcgacggcg ctgtcgtcca
     2281 cggccgggcg gtaaacgtcc atcgccagca gcacgccgtc tcgcatcggg atcatgacgt
     2341 tccgttcgac gacgaaatcg tcggcgaagg acggcgacac gaggcacatc gtcagcaaca
     2401 ccgacgccat ccccgaaacc gatagcgtca atgccggcct gcgcccggcg cgggctgtgc
     2461 agtgtttcat cggctctact ccggaagagg atccaggtac cgacgtt
//
LOCUS       DATDAA010000021         6862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_455733,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000021 DATDAA010000000
VERSION     DATDAA010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455733"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1808
                     /locus_tag="VIS73_00720"
     CDS             <1..1808
                     /locus_tag="VIS73_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY5761707.1"
                     /translation="RAHTLYNRDQQYVVQNNEIIIVDEFTGRLMPGRRWSEGLHQAVE
                     AKEGVRIQSENQTLASITFQNYFRMYGKLSGMTGTADTEAYEFQQIYGLETVVIPTHR
                     PMIRIDNNDQVFRTGREKFEAMLGDIRERHANGQPILVGTTSIENSELLSQLLTKEKL
                     EHQVLNAKQHAREAEIVVQAGRPGVITIATNMAGRGTDIVLGGNISKLVEAIRKDESL
                     DQASREQKVAALQQEWEKAHTQVIEAGGLHIIGSERHESRRIDNQLRGRSGRQGDPGS
                     SRFYLSLEDSLLRIFAGDRLKAIMNRLNLPDGEAIEHPMVTRSLESAQRKVEARNFDI
                     RKQLLEYDDVANDQRRVIYQQRNELLITDDISETIAGMRHGVLFELFRSFVPADSVEE
                     QWDVAGLEQSLAGDYRIQAPVSEWMKAEPTLNDEAIFERIHQMAVAAYDAKLAMVDRD
                     AFRQFERNLMLQSLDSHWREHLAALDHLRQGIHLRGYAQKNPKQEYKREAFELFESLL
                     NQVRAEVTRVLLTLEIQSPDAVAEVEQPQVENVQYQHPDFAGDMAAAESEAAMAEAAV
                     GQRQTPVRATEKVGRNEPCPCGSGKKYKHCHGKLV"
     gene            1907..3148
                     /gene="argJ"
                     /locus_tag="VIS73_00725"
     CDS             1907..3148
                     /gene="argJ"
                     /locus_tag="VIS73_00725"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEY5761708.1"
                     /translation="MPVNYQTPAAETLKPVAGVALGIAEAGIRKANRRDLTLIALDPG
                     ATVAGVFTENRFCAAPVHLCRAHLAAGNAIRALVINTGIANAGTGEAGMRAANATCAA
                     VAEQLGLSGASQVLPFSTGVILEPLPVDRLIAGLPAARADLAPANWHAAAHAIMTTDT
                     VAKAASRQITVAGKTITVTGISKGAGMIRPNMATMLGFVATDAGIAQPLLDRLVREAA
                     DASFNGITVDGDTSTNDSFIVIATGASGVSVAGDADPAYAAIRTVVIDVARELAQAIV
                     RDGEGATKFISVVVEGGGDRDECRKVAYAIAHSPLVKTAFFASDPNLGRILAAIGYAG
                     IADLDVAKIRVWLAGGGEDVLVAEEGGRATSYREELGAAVMKDAEITIRVDLGRGTAQ
                     ATVWTCDFSYDYVKINADYRS"
     gene            complement(3136..5610)
                     /locus_tag="VIS73_00730"
     CDS             complement(3136..5610)
                     /locus_tag="VIS73_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5761709.1"
                     /translation="MQLDLSRRLNDIRIWIRLVIATVGSVAIIWAGFLAWATYQQKEQ
                     AVAQARDFAVSLHQTTMAGLTAMMINGTIGTRVLFVEQIKQSNHVEDLRVYRSEAVIR
                     QYGKGREGEVPGDPAELRVLADGKPYFEVIEQEPHGEGDHRARSHRHLKAIIPALALE
                     NYLGKNCLGCHEVPVGTVLGAVSMEISLDSAEQAVREFTVQSILTALALLVPLGLFIW
                     YFIARIVTRPLAKLTYGLNRIAEGDIERASRLPRRGADEIGQAAEAFNRVMGKANDLI
                     KQQRLSRIVFDNALEGITVTDDKARIQMVNRAFVETTGYSAQEAIGQTPALLRSGRHD
                     EAFYQHFWHSITDKGEWRGEIWNRRKNGSLYAEWLNVSAVRNPRGEIEHFIAIFSDIT
                     ERKQREEMITWQAYHDALTSLPNRMLFRDRLDQALAMAKRAKNRCPAVMFLDLDRFKQ
                     INDTLGHDAGDELLKEVAARLKRCVRDSDTVARLGGDEFTVLLPEVADKDSVRIVADK
                     ILESMHCPVVLAGQAVSVTTSIGIALYPQDGSDADTLMKHADAAMYQVKGAGRAGARF
                     FTAEMIGLPSRRLEMESQLRTTLDAKSFVIHYQPLISLATNTAYGAEALLRLPDASGE
                     LLLPENFLSIAEETGLIVPIGDWVIEQACAQAAAWRAAGRDLSVAINLSSRQLHRMEI
                     VETLRSALERHALPADRLQVELSETVAMRDGEFSARALAAIAHLGVRIVVDDFGLGYS
                     NLQLLQRISVHGVKIDRSFIRSCETSAEDRNVVRGVAAMAHAIGLHVIAEGVETPAQR
                     QLVDELGCDEAQGFHFAHPLPADQLR"
     gene            complement(5689..6075)
                     /gene="apaG"
                     /locus_tag="VIS73_00735"
     CDS             complement(5689..6075)
                     /gene="apaG"
                     /locus_tag="VIS73_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEY5761710.1"
                     /translation="MSTNDERYRIHVEALPQFLADESDPETDRYVFAYTITVENTGEV
                     PAQLISRHWIITDADLRVQEVRGQGVVGEQPVLKPGERFQYTSGCALNTPVGSMRGSY
                     HMRAEDGTEFDAAIPEFSLAGPRTLH"
     gene            6306..>6862
                     /gene="rpe"
                     /locus_tag="VIS73_00740"
     CDS             6306..>6862
                     /gene="rpe"
                     /locus_tag="VIS73_00740"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY5761711.1"
                     /translation="MAIIAPSLLSADFARLGEEVRNVVAAGADWIHFDVMDNHYVPNL
                     TIGPLVCQAIRPHTSAPIDVHLMVKPVDRIIPDFAKAGANIITFHPEASEHIDRSLAL
                     IRDSGCQAGLVFNPATPLDYMDYVMDKLDVVLLMSVNPGFGGQSFIAGTLPKLKAARA
                     KIDAYAKQSGRQIRLEVDGGVKVDN"
BASE COUNT         1247 a         2169 c         2209 g         1237 t
ORIGIN      
        1 tgcgcgccca cacgctgtac aaccgcgacc agcagtacgt ggtgcagaac aacgagatca
       61 tcatcgtcga cgagttcacc gggcgcctga tgcccggccg ccgctggtcg gagggcctgc
      121 atcaggctgt cgaggcgaag gaaggcgtgc gcatccagtc ggagaaccag acgctcgcct
      181 cgatcacctt ccagaactac ttccgcatgt acggcaagct ctcgggcatg accggaaccg
      241 ccgataccga agcctacgag ttccagcaga tctacggcct ggaaacggtg gtcattccga
      301 cgcaccggcc gatgatccgg atcgacaaca acgatcaggt cttccgaacc ggacgcgaga
      361 agttcgaggc gatgctcggc gacatccgcg agcgccatgc caacggccag ccgattctgg
      421 ttggcacgac ctcgatcgag aactccgaac tgctgtcgca gctactgacc aaggagaagc
      481 ttgagcacca ggtgctcaac gccaagcagc acgcgcgcga agccgagatc gtcgttcagg
      541 ccgggcggcc cggcgtcata accatcgcca ccaatatggc cggtcgcggc accgacatcg
      601 tcctcggcgg caacatcagc aagctggtag aggccatccg caaggacgag agcctcgatc
      661 aggcgtcgcg cgagcagaag gtcgcagcgc tgcagcagga gtgggagaag gcgcacacac
      721 aggtcatcga agcaggcggc ctgcacatca tcggttccga acgtcacgaa tcgcggcgga
      781 tcgacaacca gttgcgtggc cgctcgggtc gccagggcga cccaggcagc tctcgcttct
      841 atctgtcgct tgaagactcg ctgctgcgga ttttcgccgg cgatcggcta aaggcgatca
      901 tgaatcgcct aaatctgcct gacggtgagg cgatcgagca tccgatggtg acgcgctcgc
      961 ttgagtcggc gcagcgcaag gtcgaggcgc ggaacttcga tatccgcaag cagctgcttg
     1021 agtacgacga cgttgccaat gaccagcggc gcgttatcta ccaacagcgc aacgagctgc
     1081 tcatcaccga cgatatttcc gaaacgatcg ccggcatgcg tcatggcgtt ctgtttgagc
     1141 tgttccgcag tttcgtaccc gctgacagcg tcgaagagca gtgggacgtt gccggccttg
     1201 agcagtcgct tgccggcgat tacaggattc aggcgccggt cagcgagtgg atgaaggccg
     1261 agccgacgct gaacgacgag gcgatcttcg agcgcatcca ccagatggca gttgctgcct
     1321 acgacgccaa gctggcgatg gttgaccgcg acgctttccg gcaattcgag cgcaacctta
     1381 tgcttcagag cctcgacagc cactggcgcg agcacctcgc ggcgctcgac cacctgcgcc
     1441 agggaatcca cctgcgtggc tatgcgcaaa aaaatccgaa acaggagtac aagcgcgaag
     1501 ccttcgagct attcgagtcg ctgctaaatc aggtccgcgc cgaggtcacg cgcgtgctgt
     1561 tgacgctgga gatccagtcg ccggatgccg tggccgaggt cgagcagccg caggtcgaga
     1621 acgtccagta ccagcacccc gactttgccg gcgatatggc agcagccgaa tccgaagcgg
     1681 cgatggccga ggcggccgtc ggacaacgcc agacaccggt tagggcgacc gagaaggttg
     1741 gccgtaacga gccatgcccc tgcggcagcg gcaagaaata caagcactgc cacggcaagc
     1801 tcgtctgagc ggccgttgaa atctgccgca cgccagttgc attggcgtac ggcggcatgg
     1861 tgcgccgatt ccggttgcgc ctgttccgac ccaattggag acatcgatgc cggtgaatta
     1921 ccagaccccc gccgccgaaa ccctgaaacc cgtcgccggc gtcgccttgg gcatcgctga
     1981 ggcgggaatc cgcaaggcca atcggcgcga tctgaccctc atcgcactcg accccggcgc
     2041 caccgttgcc ggtgtattca ccgagaaccg tttctgtgcc gcgccggtcc acctttgccg
     2101 tgcgcatctc gctgctggca acgcgattcg ggcgctggtg attaacaccg ggatcgccaa
     2161 tgccggtacc ggcgaagctg ggatgcgcgc cgccaatgcc acctgtgcag cggtcgccga
     2221 gcaactcggc ctctccggcg cgagccaggt gctgcctttc tcgaccggcg tgattctcga
     2281 gccgctgccc gtcgatcggc tgatcgccgg ccttccggcg gcgcgcgccg atctggcgcc
     2341 agccaactgg catgccgccg cgcacgcgat catgaccacc gatacggtgg ctaaggccgc
     2401 ttcgcggcag atcacggtcg ccggcaagac gataaccgtc accggcatca gcaagggcgc
     2461 cgggatgatc cggccgaaca tggcgaccat gctcggcttc gtcgccaccg acgccggcat
     2521 tgcccagccg ctgctcgacc gattggtgcg cgaggccgca gacgcctcct tcaatggcat
     2581 caccgtcgat ggcgatacct cgaccaacga ctctttcatc gtcatcgcaa ccggtgcgtc
     2641 gggtgtgtcg gttgccggcg atgccgaccc ggcctacgcc gcgatccgta cggtggtcat
     2701 cgacgtggcc cgggagctgg cgcaggcgat cgtccgtgac ggcgagggtg cgaccaagtt
     2761 catcagcgtc gtggtcgaag gcggcggcga ccgtgacgag tgccgcaagg ttgcgtatgc
     2821 gatcgctcac tcgcctctgg tgaagacggc gttcttcgcg tccgacccca acctcgggcg
     2881 cattctggcc gcgattggtt acgctggaat tgccgatctt gatgtcgcga agatccgcgt
     2941 atggctggcc ggtggcgggg aggacgtgct ggtcgccgag gagggcggcc gcgccacaag
     3001 ctatcgcgag gagcttggcg cggcggtcat gaaggacgcc gagatcacca tccgcgtcga
     3061 ccttggacga ggcaccgcgc aggcaaccgt ctggacctgc gacttttcct acgactacgt
     3121 caagataaac gccgactacc gcagctgatc ggctggcagc gggtgcgcga agtggaagcc
     3181 ctgtgcctca tcgcagccga gttcgtcaac cagttggcgc tgcgccggcg tttcgacgcc
     3241 ttcggcgata acatgcaggc cgatcgcgtg ggccattgcc gcgacgccgc ggacgacatt
     3301 gcggtcctcg gctgaggttt cgcagctgcg aatgaagctg cggtcgatct tcacgccgtg
     3361 caccgagatc cgctgtagca gctgcagatt agaatagcca agaccgaagt catcgacgac
     3421 gatgcgcact ccgaggtggg cgattgcggc cagcgctcgt gccgagaatt cgccgtcgcg
     3481 catcgccacc gtttccgaga gctccacctg cagccggtcg gccggcagcg catggcgttc
     3541 gagcgcgctg cgcaaggttt cgacgatttc catccgatgc agctgccgcg acgacaggtt
     3601 gatcgcgacg ctgaggtctc ggccggcagc gcgccaggcc gctgcctgcg cgcaggcctg
     3661 ctcgatcacc cagtcgccga tggggacgat cagcccggtt tcctcggcga tcgacaggaa
     3721 gttttcgggc aagagcaact cgcccgaggc gtcgggcagg cgcagcaggg cttcggcgcc
     3781 ataggcggta ttggtcgcca gcgagatcag cggctggtag tggatcacga agcttttggc
     3841 atcgagtgtg gtgcggagct gcgattccat ctccagacga cgcgagggca ggccgatcat
     3901 ctcggctgtg aagaagcgcg cgccggcgcg tccggcgccc ttgacctggt acatcgcggc
     3961 gtcggcgtgc ttcatcagcg tatcggcgtc gctgccgtcc tgcggataaa gggctatgcc
     4021 gatgctcgtg gtgaccgaca ccgcctggcc cgcaagcacg accggacagt gcatcgactc
     4081 aaggatctta tcggcgacga tgcgcacgct gtccttgtcg gccacttcgg gtagcagcac
     4141 cgtgaactcg tcaccgccga gccgggcgac cgtatccgaa tcgcggacgc aacgcttgag
     4201 acgggcggca acctccttga gcaactcgtc gccggcgtcg tgaccgagcg tgtcgttgat
     4261 ctgcttgaag cggtcgaggt cgaggaacat taccgccggg cagcgattct tggcgcgttt
     4321 ggccatcgcc aaggcctgat cgaggcgatc gcggaacagc atgcggttgg gcaggctggt
     4381 gagggcgtcg tggtaggcct gccaggtaat catttcctcg cgctgcttgc gctcggtgat
     4441 gtcgctaaag atcgcgatga agtgttcgat ttcgccgcgc gggttacgga cggccgagac
     4501 attgagccac tcggcgtaga gcgaaccgtt cttgcggcga ttccagatct cgccgcgcca
     4561 ctctcccttg tcagtgatcg aatgccagaa atgctgatag aaggcttcgt cgtgacgccc
     4621 cgaacgcagc agcgccggcg tctggccgat cgcctcctgc gccgagtatc cggtggtctc
     4681 gacgaaggcg cgattgacca tctggatgcg ggccttgtcg tcggtgaccg tgatgccctc
     4741 aagcgcgttg tcgaagacga tgcgcgacag gcgctgctgt ttgatcaaat cgttggcttt
     4801 gcccattacg cggttgaagg cctcggccgc ctgaccgatt tcgtcggcgc cacggcgcgg
     4861 cagccggctg gcgcgttcga tgtcgccttc ggcaatgcga ttgagaccgt aggtcagctt
     4921 ggcaagcggg cgggtgacga tgcgggcaat gaagtaccag atgaacagcc ccagcggcac
     4981 gagaagcgcg agcgccgtga ggatcgactg caccgtgaac tcccggaccg cctgctcggc
     5041 gctgtcgagc gaaatttcca tgctgaccgc gccgagaacc gtgccgaccg gcacttcatg
     5101 gcacccgagg caattcttgc ctaggtagtt ttccagcgcc agcgccggaa tgatcgcttt
     5161 caggtgccgg tgggagcggg cgcgatggtc cccttcgcca tggggctcct gctcaatgac
     5221 ttcgaagtag ggcttgccat cggccagtac gcgcaattcg gcggggtcgc ccgggacttc
     5281 gccctcgcgg cccttgccgt attgccggat cacggcttcc gagcggtaga cccgcaggtc
     5341 ctcgacgtgg ttggactgtt tgatctgttc tacgaagagc acgcgtgtgc cgatcgtgcc
     5401 gttgatcatc atcgccgtga ggccggccat ggtcgtctgg tgaaggctta cggcgaaatc
     5461 gcgtgcctgc gcaaccgcct gctctttctg ctggtaggtg gcccaagcca ggaaccctgc
     5521 ccagatgatc gcaaccgaac cgaccgtcgc gataaccaag cgaatccaga ttcttatgtc
     5581 gttgagccga cgcgataggt cgagttgcat cagcgtcggt ttccccagtt ccgccctgtc
     5641 ttggtgctag gcgcggattg tagccgaagc cctctaccgg gggtgctctc agtgcagtgt
     5701 tcgcggcccc gccagcgaga attcagggat cgctgcatca aactcggtac cgtcttcggc
     5761 gcgcatgtgg taactgccgc gcatgctgcc gaccggcgtg ttgagtgcgc aacccgaggt
     5821 gtactgaaac cgttcgccgg gcttgaggac cggttgttca ccgaccacgc cctgcccgcg
     5881 gacttcctgt acgcgcagat cggcgtcagt gatgatccag tggcgcgaga tcagctgggc
     5941 cggaacctcg ccggtattct cgaccgtgat cgtataggcg aatacgtagc ggtcggtctc
     6001 ggggtcggat tcgtcggcga gaaattgcgg cagcgcctca acgtgaattc ggtatcgctc
     6061 atcgtttgtg ctcatcgagt gtccgggcct ttctaattga attgtcgctt cgacaggggt
     6121 ttgtcgcgaa tcgttccggc cgagggctca aggcccttac gccgtatggg ttacccgcgc
     6181 ggaggatcgg gcgcagacag ggcgggcggc tagaatggcg gtcgtctggc tgggcggttg
     6241 gtgttgaccg aacccggccg ccgcgcggca gagatcgtag ccaccgaatt cgtgaggaga
     6301 agcccatggc gatcatcgcc cccagtctct tgtctgccga cttcgcccgt ctcggcgagg
     6361 aagtccgtaa cgtcgttgca gccggagccg actggattca cttcgacgtc atggacaacc
     6421 attacgtccc caacctgacg attggcccgc tggtctgcca ggcgatcagg ccgcacacca
     6481 gtgcgccaat cgacgtgcac ctgatggtca agcccgtcga tcggatcatt cctgatttcg
     6541 ccaaggccgg cgccaacatc atcaccttcc acccggaggc ctccgagcac atcgatcgca
     6601 gcctcgcgct gatccgtgac tcgggctgcc aggccggctt ggtcttcaat ccggcgacgc
     6661 cgctcgacta tatggactac gtcatggaca agctcgatgt cgtgctgctg atgagcgtga
     6721 accccggctt cggcgggcag agtttcatcg ccggcacgct gcccaagctc aaggcggcgc
     6781 gcgcgaagat cgatgcctac gccaagcaga gtggccggca gatccggctc gaagtcgacg
     6841 gcggcgtcaa ggtcgacaac at
//
LOCUS       DATDAA010000022        10518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_385797,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000022 DATDAA010000000
VERSION     DATDAA010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10518
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385797"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VIS73_00745"
     CDS             <1..251
                     /locus_tag="VIS73_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761712.1"
                     /translation="RYDEAVRVLGAINKPDWANRARWLAASYAAAGHIEEARAQIVAL
                     LKIDPDESLSRLRKTLPWKNPQDLERELTHLKLAGLTE"
     gene            467..1015
                     /locus_tag="VIS73_00750"
     CDS             467..1015
                     /locus_tag="VIS73_00750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY5761713.1"
                     /translation="MSHFLFLNASTREPGQVGNTETLARRAATALTPETIQTWHALAS
                     LDLPPFVDRRHTTGIYAMPEGDARMLLDATLAASDLVLVSPVYWYSVPSPLKHYLDQW
                     SAWMRVPGLDFKARMAGKRLWVASTSGDRAKAQPMIDSYRLCAEFLSMKWQGVLWGKG
                     GAPGAVLDDAEALAAADTFFGG"
     gene            1023..1523
                     /locus_tag="VIS73_00755"
     CDS             1023..1523
                     /locus_tag="VIS73_00755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5761714.1"
                     /translation="MAPRLATPADFDSVLALNTESERFLSPLDAARLAQLDAEAERHW
                     VIEAAGRVVAFVLVFREGADYDSVNYRWFVARCPKFLYVDRIVVSPTYRGRGIAAQLY
                     RQVFEHAAASGVPWVAAEYDIEPPNPGSAHFHAALGFREVGRQAVADGRKSVTLQVTL
                     ADDSAC"
     gene            1523..2272
                     /locus_tag="VIS73_00760"
     CDS             1523..2272
                     /locus_tag="VIS73_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY5761715.1"
                     /translation="MVSSESLVLIAAVFLLAGWVKGIVGMGLPTVVMGALALAMPPAE
                     AAALLIVPSLATNLRQFLAGPSPWKLARRMASLLLFICIGTALGIPLLTASATHWPAL
                     ALGAVLAVYALIGLFARPFVLPPDAARRWAPAVGGLTGVLTGATGVFVVPAVPYLSAL
                     GLSRDELVQALGISFTVSTIALGVALGVRTSYSPTLLALSVGAVLPALLGMALGQATR
                     SRIAQAVFRRWFFTAMLAVGLTMLARGLLAM"
     gene            2381..3319
                     /gene="hemC"
                     /locus_tag="VIS73_00765"
     CDS             2381..3319
                     /gene="hemC"
                     /locus_tag="VIS73_00765"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY5761716.1"
                     /translation="MTKPARIIIASRESRLAMWQAEHVRDRLAAAHPGAAVSILGMTT
                     KGDQILDRPLAEIGGKGLFIKELEVAMAEGRADLAVHSMKDVPMDMPEGFVLAAISAR
                     ENPRDAFVSGKYERLSDLPAGAVVGTSSLRREATLRANHPQLQIRSLRGNLDTRLRKL
                     DEGQYDAIILAAAGLIRLGLGDRVRSVLSPEESLPAPGQGALGIEIREGRDDMMAAVA
                     TLNDAHSAACVVAERAFSRALGGSCQLPLGGYGEIKNGQIWLRGFVATADGQKFVAGE
                     TWGAPADAASLGQSLADELRGRGADAILAELAAAKH"
     gene            3342..4169
                     /locus_tag="VIS73_00770"
     CDS             3342..4169
                     /locus_tag="VIS73_00770"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY5761717.1"
                     /translation="MAVESQRATEPALLAGRAILITRPLAQARPLAAAIESAGGHARI
                     FPLLRIEPIGESAARDEAIAGLRDAAFAAFVSPNAVAASVPRILERGPWPAGTQAVAV
                     GAGTAKALAALGVGPVIVPESRFDSEALLELPALAAAQVAEQRVLIFRGDGGRELLAD
                     ELRARGARVDCISCYRRLPPADFAPLVDWIDAGALDALSVTSSEALRHLEAGLDTARR
                     DRLHALPFFTSHERIAGNAAEAGFRIIVKTDAGDAALIAGLCAYNWPSIGGPPASAS"
     gene            4254..5405
                     /locus_tag="VIS73_00775"
     CDS             4254..5405
                     /locus_tag="VIS73_00775"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY5761718.1"
                     /translation="MTDNQEQTPAGADSAAAKSDLPLPAPAETKEARGAKAAKAEETR
                     DRPRRNFAGLWVLLAIAALALAAWQWAETRGRLTVTQQELAARLAEQDRELGEARVAA
                     KEARETGESAKARLAVIEAKLAEFQAQYAALDAMYQDVARGRDESVISDAEQLVGLAA
                     QQLQLAGNASGAVLALTQADLRLAKIDRAQLANIRRLLARDIERLRSLPEVDLVSVSV
                     KIESVVESVDALSPASQMRPSGALPPPEPDATAAEEANIVERLRRFARGIWEELRSLV
                     RIQRFDRLEPVLLPPTQEYFLRENLKLRLLNARLALLSRDGNTFRHEILQAKAWIERH
                     FDTRQRPAAQAIATLSQLGALDIVVRLPRLDETLAAIRAVKLDHSRSGR"
     gene            5402..6583
                     /locus_tag="VIS73_00780"
     CDS             5402..6583
                     /locus_tag="VIS73_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HEY5761719.1"
                     /translation="MKWLFWLLVIFAVAALAAVGAQVNTGYALIVWAPYRVEVSLNLV
                     VVVLLVAFLLFYWALRSLASVFGLPRQVAAYRARRRQEKGQQVFHDAFRLLFEGRFGQ
                     SLKKAGESFEAGIDRGLSALIAARAAQRMRRNDDQQLWLERAGAADRETQPARLMLEA
                     EMCLDGRDFKGAIKALDALKKISGRNLAAQRLELRARQGVGDWPGVLELARHLEKKDV
                     LIPEVSGELKLRAHRENILARKGDARAIQAYLRDIPSAEMSSRLTKTAATALIEDGAC
                     EEAAKVIEAQLTRGWDAPLAAIYGQCGGERITARIAEAEKWLRQHPDDAGLLLALGRL
                     CLQQQLWGKAQSYLEASLSIEESRDAHLELARLADRLGQKAEADRRYRLASKPELCHC
                     N"
     gene            complement(6641..7537)
                     /gene="hemF"
                     /locus_tag="VIS73_00785"
     CDS             complement(6641..7537)
                     /gene="hemF"
                     /locus_tag="VIS73_00785"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEY5761720.1"
                     /translation="MDNTAVRDWLAGLQQSIAGQAGALDGTAFRSDAWDRPEGGGGRT
                     MIVEGGQLFERAGVAFSHVMGKAMPASATQHRPELAGRAFEAMGVSLVMHPLNPYCPT
                     AHMNVRCFVATKPDAEPVWWFGGGMDLTPYYPFTEDVVHFHRSCRDTLAPFGDGVHAR
                     YKRWCDEYFFLKHRQEPRGVGGIFFDDLNEGGFERCFSLVRAVGESFAGAYFPLLERR
                     RDTAYGERERDFQAYRRGRYVEFNLVWDRGTLFGLQSGGRTESILLSMPPVVTWRYDW
                     KPEAGSPEAALYADFLPPRDWV"
     gene            complement(7537..7989)
                     /locus_tag="VIS73_00790"
     CDS             complement(7537..7989)
                     /locus_tag="VIS73_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY5761721.1"
                     /translation="MAFERYLDDFTPGERFTTPGVTFTEAEIIDFALKYDPQAFHLDA
                     SAAADSLYGGLIASGYQTLALAFRMFIQSGMLAACSMGSPGIDELRWLAPVRPGDTLH
                     TEAEVLEVKPSTKKPDRGILRLKYVATNQHGDAVLSFIVNHFLKRRPA"
     gene            complement(8071..8601)
                     /locus_tag="VIS73_00795"
     CDS             complement(8071..8601)
                     /locus_tag="VIS73_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761722.1"
                     /translation="MHLLLRLLTGTDRRSIGRSDLAAQRVLRQPRLLSVLFDGVRPDD
                     PVLRMRCADAIEKATRTRPSLLKPYRAQLLGRYAAIEQPEVRWHVAAMLGRLQLSARY
                     EACAVKPLLAFTTDQSRIVRTLAMQSLADLALSNPAIQSQVLPRLKELTTTGTPAMRA
                     RGRRLLRILEPTAASG"
     gene            complement(9223..9600)
                     /locus_tag="VIS73_00800"
     CDS             complement(9223..9600)
                     /locus_tag="VIS73_00800"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEY5761723.1"
                     /translation="MKIRRFIALSVGALVVGGLAGCGAKEVSYAASVRPVLEKHCVEC
                     HKPGGEGFEKSGQDMTSYSALMKGTKFGATVKANDSFTSALVMLIEGRADPSIKMPHN
                     REPIPADDIKAIKTWIDQGAKDN"
     gene            complement(9821..10216)
                     /locus_tag="VIS73_00805"
     CDS             complement(9821..10216)
                     /locus_tag="VIS73_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEY5761724.1"
                     /translation="MQAIVRKTLGLAADKTLYERLGGNPELEAVVGELWAITSADARI
                     NHYFAKTKPEVFAGLMVDFLCQAAGGPCQYKGKDMAGAHTGMKRSEADFNALAENAAK
                     ALDKFKVPEKEHAEVMSMLASLKSTVINH"
     gene            complement(10292..10438)
                     /locus_tag="VIS73_00810"
     CDS             complement(10292..10438)
                     /locus_tag="VIS73_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761725.1"
                     /translation="MQNYPKKLLVIIAEAALEQVLSTARRAAPASTSMRARRDGAERA
                     IPSA"
BASE COUNT         1663 a         3407 c         3554 g         1894 t
ORIGIN      
        1 ggagatacga tgaggcggtg cgagttctcg gcgccatcaa caagccggac tgggccaatc
       61 gagcgcgctg gcttgccgcg agctacgcgg cggccggtca catcgaggaa gctcgcgcgc
      121 agatcgtcgc cttgctcaaa atcgatcccg acgaatcgct gtcgcgcctg cgcaagacgc
      181 taccgtggaa gaacccacag gatctggagc gcgagctgac acatctcaag cttgcagggc
      241 tgacggagta agccgcatgc ggaggaaata tacgtttggg cgctcggacc agtcaccggc
      301 cgtagaacct cagataccgc gtaggaccgc tgagggtcgg attgcgacgc tcggtgagca
      361 actgagagca acggcgcgtg gtcgtgtgtc gcgcaatgcc acatggctcg gggcgggcat
      421 cgctttcagg aggtattctc ttctgccccg atagtcctcc aaagccatgt cccattttct
      481 cttcctcaat gccagcaccc gcgagccggg gcaggttggc aataccgaaa ctctggcgcg
      541 gcgtgcggcg acggcgctga cgccggaaac aatccagacc tggcacgcac tggcatcgct
      601 tgacttgccg cccttcgtcg atcgtcggca tacaaccggc atctacgcga tgcccgaggg
      661 cgatgcccgg atgctgctcg acgcgacgct ggccgccagc gatctggtgc tggtgtcgcc
      721 ggtctattgg tactcggtgc catcgccgct caagcattac ctcgatcagt ggagtgcctg
      781 gatgcgggtg cccggtctcg acttcaaggc gcgcatggcc ggcaagcggc tgtgggtggc
      841 aagcaccagt ggcgaccgcg cgaaggcgca gccgatgatc gacagctatc ggctgtgtgc
      901 cgaatttctg tcaatgaaat ggcagggcgt gctctggggc aagggcggtg cgccgggtgc
      961 ggtgctcgac gatgccgagg cgctggccgc tgccgatacg ttcttcggtg gatagtcacg
     1021 agttggcgcc gcggttagcg acgccggcgg attttgactc ggtactcgcg ctcaacaccg
     1081 agtccgagcg ctttctcagc ccgctcgatg cggcgcggct ggcgcagctc gatgccgagg
     1141 ccgagcggca ctgggtgatc gaggcggcgg gccgcgtcgt cgccttcgtg ctggtctttc
     1201 gtgaaggagc cgactacgac agcgtgaact accgctggtt tgtggcgcgt tgcccgaagt
     1261 ttctctacgt cgatcgcatc gtcgtgtcgc cgacctatcg cgggcgcggg attgccgcgc
     1321 agctgtatcg gcaggtcttc gagcacgcgg cggcaagcgg cgtgccctgg gttgccgccg
     1381 agtacgacat cgagccgccg aatccgggct cagcacactt ccacgccgcg ctcggcttcc
     1441 gcgaggtcgg gcggcaagcg gtcgccgatg gccgcaagtc ggtgacgctg caggtcacgc
     1501 tggcggacga cagcgcttgc tgatggtctc gtccgagtcg ctcgtgctga ttgctgcggt
     1561 atttctgctc gccggctggg tcaagggcat cgtcggcatg ggcctgccga cagtggtgat
     1621 gggggcgctg gcgctagcga tgccaccggc cgaagcggcg gcgctgctta tcgtgccgtc
     1681 gctggccacc aatctcaggc agtttctcgc cgggccctcg ccgtggaaac tagcgcggcg
     1741 gatggcaagt ctgttgctgt tcatctgtat cggaacggcg ctcggcattc cgctgctcac
     1801 tgccagtgcg acgcattggc cggcgctcgc gctcggcgcg gtgctggcgg tttacgcgct
     1861 gatcggcctc tttgcaaggc ccttcgtgct gccgcccgat gcggcgcgac gctgggcccc
     1921 ggcagtcggc ggcctgaccg gcgtgctcac cggcgccacc ggcgtcttcg tggttccggc
     1981 ggttccctac ctcagtgcgc tcggtctgag tcgcgatgaa ctggtgcagg cactcgggat
     2041 ttccttcacg gtctcgacga ttgcgctcgg tgtcgcgctc ggcgtgcgca cgagttattc
     2101 gccgacgctg ctcgcgctct cggtcggcgc cgtgctgccg gcgctgctag gcatggcgct
     2161 cggacaggcg acgcgcagcc ggatcgcgca agcggttttt cgtcgctggt tcttcacggc
     2221 gatgctcgcg gtcggcctca ccatgctggc gcgggggctg ctcgcaatgt aggcgcgtgc
     2281 ggtcggttcg ccacgttgcg ctcgaccggg agacgacttt gcccctgcta caatcgcgcg
     2341 cttctccggc agccctgccg agccctccgc gatcgccgcc atgaccaaac ccgcacgcat
     2401 catcatcgcc tcgcgcgaat cccgtcttgc catgtggcag gccgaacacg tccgcgaccg
     2461 tctcgccgcc gcgcaccccg gcgccgccgt gtcgattctc ggcatgacca ccaagggcga
     2521 ccagatcctc gatcggccac ttgccgagat cggcggcaag gggctgttca tcaaggaact
     2581 cgaagtggcg atggccgagg gccgcgctga cctcgccgtg cattcgatga aggacgtgcc
     2641 gatggacatg cccgagggtt tcgtcctcgc ggcgatctcg gcgcgcgaga atccgcgcga
     2701 cgccttcgtt tcgggcaagt acgaaaggct ttccgacctg ccggccggcg ccgtcgtcgg
     2761 cacatcgagc ctgcgacgcg aggcgacgct gcgcgccaac catccgcagt tgcagatccg
     2821 cagcctgcgc ggcaacctcg acacgcggtt gcgtaagctc gacgaaggcc agtacgacgc
     2881 gatcatcctc gccgctgccg ggctgatccg cctcggactc ggcgaccgcg tgcgctcggt
     2941 gctctcgccc gaggagtcgc tgccggcgcc gggtcagggc gcgctcggca ttgaaatccg
     3001 cgaagggcgc gacgacatga tggccgccgt tgccaccctt aacgatgcgc attcggctgc
     3061 ctgcgtggtc gccgagcgcg ccttctcgcg tgccctgggc ggcagctgcc agttgccgct
     3121 gggtggctac ggggagatca agaacggcca gatctggctt cgcggctttg tcgccaccgc
     3181 cgacggtcag aagttcgtcg ccggcgaaac ctggggggcg ccggccgatg ccgcatcgct
     3241 cggtcaatca ctcgctgacg aattgcgcgg ccggggcgcc gacgcgatcc tcgccgaact
     3301 cgccgccgcg aaacactgag cgccaaggcg tcggagccga catggcggtc gagtcgcagc
     3361 gcgcaaccga gcctgcgctg ctcgccggcc gcgcgatact gatcacccgg ccgttggcgc
     3421 aggcgcgtcc attggccgcg gcaatcgaat ctgccggcgg ccacgcccga atctttccgc
     3481 tgctgcggat cgagccgatc ggcgaatcgg cggcgcgtga cgaggccatc gccggattgc
     3541 gcgatgccgc cttcgccgcc ttcgtcagtc ccaatgccgt tgccgccagc gtgccgcgga
     3601 ttctcgaacg cgggccgtgg ccggcgggca cgcaggcagt tgccgtcggt gccggtacgg
     3661 cgaaggcgct cgccgcgctc ggcgtcgggc cggtgatcgt tcccgagtcg cgcttcgaca
     3721 gcgaggcgct gctcgagttg ccggcgctgg ccgcggcgca ggtggccgag caacgcgtgc
     3781 tgatcttccg cggcgacggc ggtcgcgaat tgctggccga cgaactgcgc gcacgcgggg
     3841 cgcgagtcga ttgcatcagc tgctaccggc gcctgccacc ggccgacttc gctccgctgg
     3901 ttgactggat cgatgccggc gcgctcgatg cgctgtcggt caccagcagc gaggcactgc
     3961 ggcatcttga ggccgggctt gacaccgccc gacgtgaccg tctgcacgcg ctacccttct
     4021 tcacctcaca cgaacgcatc gccggaaacg cggccgaggc cggatttcgg atcatagtca
     4081 agactgatgc cggcgacgcg gccttgatcg ctgggctctg tgcttataat tggccgtcca
     4141 ttggcgggcc gcctgccagc gcgagctgaa acaggacgcg tgcgccggca acgccaaact
     4201 ccgcgccccc gcagcacggg aacgcaagcc gatccaccga ttccagaaat gcaatgaccg
     4261 ataaccaaga acagactccg gccggcgccg attccgccgc cgccaagagc gaccttccgc
     4321 tgccggctcc ggccgaaacc aaggaagcta ggggcgccaa ggcggccaag gccgaggaga
     4381 cgagggatcg tcctcgccgc aatttcgccg gcctctgggt cctgctggcc attgccgcgc
     4441 tggcgctcgc cgcctggcag tgggcagaga cgcgcggacg gctaaccgtt acgcagcagg
     4501 aacttgccgc acgcctcgcc gagcaggacc gcgaactcgg tgaagcccgt gtcgccgcca
     4561 aggaggcacg cgaaaccggt gagtcggcca aggcgcggct ggcggtgatc gaggccaagc
     4621 tcgccgagtt ccaggcgcag tacgcggcgc tcgacgcgat gtatcaggat gttgcccgcg
     4681 gtcgtgacga atcggtcatt tcagatgccg agcaactggt cggacttgcc gctcaacagt
     4741 tgcaactcgc cggcaatgcc tcgggtgccg tgctggcgct gactcaggcc gacctgcggc
     4801 tggccaagat cgatcgcgcc cagctggcca atatccgccg cctgctggcg cgtgatatcg
     4861 aacggctgcg cagtctgccc gaggtcgatc tggtttcggt cagcgtcaag atcgagtccg
     4921 tcgtcgagtc cgtcgacgcg ctgtcgccgg cgagccagat gcgcccttcg ggcgctctgc
     4981 cgccacccga acccgatgcg acagcggccg aggaagcgaa tatcgtcgag cgcctgcgcc
     5041 gctttgcgcg cggcatctgg gaagaactgc gcagcctcgt ccgtatccag cgattcgatc
     5101 gtctcgagcc ggtgctgctg ccgccgacgc aggaatactt cctgcgcgag aacctcaagc
     5161 tgcgcctgct caatgcgcgg ttggcgctgc tctcgcgtga cggcaacacc ttccgccacg
     5221 aaatcctgca ggcaaaggcg tggatcgagc gccacttcga tacgcgccaa cgaccggcgg
     5281 cgcaggcaat cgccacgctc tcgcagctcg gcgcgctcga catcgttgtc cgcctgccgc
     5341 gcctcgacga gacgctggcg gcgattcgcg ccgttaagct cgatcactcg cggagcggcc
     5401 gatgaagtgg cttttctggc tactggtcat tttcgcggtc gcggcgctgg ccgcggtcgg
     5461 tgcgcaggtc aataccggtt acgcgttgat cgtctgggcg ccctatcgcg tcgaggtgtc
     5521 gctcaatctg gtcgtcgtcg tgctgctcgt cgccttcctg ctgttctact gggcgctgcg
     5581 ctcgctcgcc tcggtgttcg gcctgccgcg gcaggttgcc gcgtaccgcg cgcggcgccg
     5641 gcaggagaag gggcagcagg tcttccatga tgcctttcgc ctgcttttcg aaggccgctt
     5701 cggccaatcg ctgaagaagg ccggcgagtc cttcgaggcc ggcatcgacc gcggcctctc
     5761 ggcgctgatc gccgcccgtg ccgcgcagcg gatgcgtcgc aacgatgacc agcagctgtg
     5821 gctcgagcgt gccggcgcgg ccgatcgcga gacgcagccc gcgcggctaa tgctcgaggc
     5881 cgaaatgtgt ctcgacggcc gcgacttcaa gggcgcaatt aaggcactcg atgcgctgaa
     5941 gaaaatctcc ggtcgcaacc tcgccgcgca acgcctcgaa ctgcgagcgc ggcagggcgt
     6001 gggcgactgg cccggcgtgc tcgaactcgc gcggcacctg gagaagaagg atgtgctgat
     6061 ccccgaggtt tccggcgagc tgaagctgcg cgcgcaccgc gagaacatcc tcgcgcgcaa
     6121 gggcgacgcg cgtgccatcc aggcttacct gcgcgacatc ccgtccgccg agatgtcgtc
     6181 gcggctgacc aagacggcgg cgacggcgct gatcgaggac ggtgcctgcg aggaagcggc
     6241 aaaggtcatc gaggcgcagc tgacacgcgg ctgggacgca ccgctggcgg ctatctacgg
     6301 ccagtgcggc ggcgaacgca tcaccgcgcg catcgccgag gccgagaagt ggctgcgcca
     6361 gcatcccgac gatgccggcc tgctactggc gctgggccgc ctgtgcctgc agcagcaact
     6421 ctggggcaag gcgcagagct acctcgaggc ctcgctgtcg atcgaggaat cacgcgatgc
     6481 gcatctcgaa ctggcgcgtc tcgcagaccg actcggacag aaagccgaag ccgatcggcg
     6541 ctaccggctc gcctccaagc ccgaactctg ccactgcaat tgagagtact ttgattggat
     6601 tcggccggca gccgccgacc gaatcgccgc gtggacttgc tcagacccag tcgcgcggcg
     6661 gcaggaagtc ggcatacagc gccgcttccg ggcttcccgc ctcgggcttc cagtcgtagc
     6721 gccaggtcac caccggcggc atcgacaaca ggatcgattc ggtccgtccg cccgattgca
     6781 ggccgaacag tgtgccgcgg tcccagacca ggttgaactc gacgtaacgc ccgcgccggt
     6841 aggcctggaa gtcgcgctcg cgttcgccgt aggccgtgtc gcgccggcgc tcgagcagcg
     6901 ggaagtaggc cccggcgaat gattcaccga ccgcgcgaac cagcgagaag cagcgctcga
     6961 aaccgccctc gttgagatcg tcgaagaaga tgccgccgac gccgcgcggc tcctgccggt
     7021 gcttgaggaa gaaatactcg tcgcaccagc gcttgtagcg cgcgtgcacg ccgtcgccga
     7081 agggcgccag cgtgtcgcgg cagctgcggt ggaaatgcac gacgtcctcg gtaaacggat
     7141 agtaaggcgt caggtccatg ccgccgccga accaccagac cggctcggca tcgggcttgg
     7201 tcgcgacgaa gcagcgcacg ttcatgtgcg cggtcgggca gtaggggttt agcggatgca
     7261 tcaccaacga cacacccatc gcctcgaagg cgcggcccgc cagttccggc cggtgctgcg
     7321 tcgccgaggc cggcatcgcc ttgcccatca cgtgcgagaa ggcgacgccg gcgcgttcga
     7381 agagctggcc gccctcgacg atcatcgttc gaccgccgcc gccctcgggt cggtcccagg
     7441 catcgctgcg aaaggccgtg ccgtcgagcg cgccggcctg gccggcaatc gactgctgca
     7501 gcccggcgag ccagtcgcgt accgctgtgt tgtcgatcac gccggccgcc gcttgaggaa
     7561 atggttgacg atgaaggaga gcaccgcgtc gccgtgctgg ttggtcgcca cgtacttgag
     7621 ccgcaggatg ccgcggtcgg gctttttcgt cgagggcttt acctcgagca cctcggcctc
     7681 ggtatgcagc gtgtcgccgg gccgcaccgg tgccagccag cgcagttcat cgatacccgg
     7741 cgagcccatg ctgcaggccg cgagcatgcc gctctggatg aacatgcgga aggcgagcgc
     7801 cagtgtctgg tagccactgg cgattagccc gccgtacagc gaatcggccg ccgccgaggc
     7861 atcgagatga aaggcctgcg ggtcgtactt gagtgcgaaa tcgatgatct cggcttcggt
     7921 aaaggtgacg cccggcgtcg tgaatcgctc gccgggggtg aagtcgtcca gataacgttc
     7981 gaaggccatg ggtgatttct cattattgac aaggcgtgga gcgtcggccc cgccgtttga
     8041 agtgtcaagc ttcccggggt tcccgctgcc ttagcctgat gcggcagtgg gttcgagaat
     8101 acgaagtagt cggcgtcccc gggcgcgcat cgccggtgtt ccggtggtcg ttagttcctt
     8161 cagtcgcggt aagacctgag attgaatcgc cggattgctg agggcaaggt cggcaagaga
     8221 ctgcatcgcc agtgttcgga cgatacggct ttggtcggtc gtgaaggcaa gcagaggttt
     8281 caccgcgcac gcctcgtatc tcgcggatag ctgcaggcgc ccgagcattg ccgcgacgtg
     8341 ccagcgcact tcgggttgtt cgatcgctgc gtagcgtccc agcagctgtg cccgatacgg
     8401 tttgagcagg ctggggcggg ttcgcgtggc tttttcaatg gcgtcggcgc aacgcatccg
     8461 caggaccgga tcgtctggcc ggacgccgtc aaacaagacc gacaagagac gaggttgccg
     8521 cagcacccgt tgcgcggcga gatccgagcg cccaatcgac cggcgatcgg tgccggtgag
     8581 cagtctcagc aatagatgca ctgcgctccc cctcattggc gacggaccgt gcagggcata
     8641 cgcgtcgaat cgtgggcaga agatggatac ggtacggcgc cttgggttcc gtaccgtcgt
     8701 gctgcaagcc cgcggtttgg ccgcgattcg tggttgatga gtgcgatctt caggctaccg
     8761 agcatggcca tcacttcgcc gtgctcttac gccgggacct tgaacttgtt gcgggccttg
     8821 gcggttttct cggtgagggc ctcgaagtct gcttctgaaa gcttcatgcc ggtatgtgcg
     8881 ccgaacaggg aaacctcggg gtttgccccc tttgttctct gccgacaaac cggtgtcagc
     8941 ttgctgagtg gaggcagtcc gctagtggaa tcaccgcgat gaggtcttcg cgtgccgatc
     9001 tcgttcggct aagcgcgaaa agcggcgacc ttcgccgtgg tgcagtcggc tttctgagcc
     9061 ttccgctgcg ggtgtgacgc agcgcgcagc agcccgacct ttagggggcg cgcgtacgag
     9121 gtccagcaaa ccacgctcta cgccgcctga catgcgctgg cgctgcagcg gcgttggctg
     9181 gtcgaagtcg gtatcgacta gaaatgtgcc gccgtcgcgg ggtcagttgt ctttggcgcc
     9241 ttggtcgatc caggtcttga tggccttgat gtcgtcagcc gggatcggct ccctgttgtg
     9301 cggcatcttg atcgacgggt cggcacgacc ttcgatcagc atgacgagcg cgctggtgaa
     9361 gctgtcgttc gctttcacgg ttgcgccgaa cttcgttccc ttcatcagtg cgctgtagct
     9421 ggtcatatcc tgaccgctct tttcgaagcc ctcgccgccc ggcttgtggc actcgacgca
     9481 atgcttctca agcacgggcc gtacactggc cgcgtaactg acttccttgg cgccacatcc
     9541 ggcgagaccg ccgactacaa gcgcgccgac actgagcgcg atgaatcttc ttattttcat
     9601 gatgcccccc tttgttgctg gttagcgagt ccaaaggctc gctttcatta taagcaagtg
     9661 ctgaaaaagt tcagcccgtt ttagggggcg ccgggcgctt ttcgtcgtgc cacgggaatg
     9721 gataggtttg accgcaactg ccaaacgaga acggcacgcg gcccttgcgg gcgcgtgccg
     9781 tggtgggcga tggcggccga gcggccgcgc ctgcctgggc ttagtgattg atgacggtgc
     9841 tcttcaggct ggcgagcatg ctcatcactt cggcgtgctc tttctccgga accttgaact
     9901 tgtcgagggc cttggccgcg ttttcggcga gtgcgttgaa gtccgcttcg gagcgcttca
     9961 tgccggtgtg tgcgccggcc atgtccttgc ccttgtactg gcaggggccg ccggccgcct
    10021 gacagaggaa atcaaccatc aggccggcaa agacttcggg cttggtcttg gcgaagtagt
    10081 ggttgatgcg cgcgtcagcc gaggtgatag cccacagttc gccaaccacg gcttcgagct
    10141 ccgggttgcc accgaggcgc tcgtagagag tcttgtcggc ggcgaggccg agggtcttgc
    10201 ggacgatggc ttgcatgagg ttttccgttt ggtcgtgggg tgaggcggga gcgatgctag
    10261 gggccgcccg aaccggagtc ggtgatctac gttacgcaga tggaatggcg cgttccgcgc
    10321 cgtcgcggcg ggcgcgcatc gaagtgctcg cgggtgcggc ccggcgagcc gtgctcagaa
    10381 cttgctcgag cgctgcctcg gcgatgatga ccaagagctt tttcggatag ttctgcatgc
    10441 gctagctccg cagccgttca gcaatcgcga tgtagagcgg gatgccgacc atgatgttga
    10501 aggggaaggt gattccca
//
LOCUS       DATDAA010000023         6693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_71580,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000023 DATDAA010000000
VERSION     DATDAA010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71580"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VIS73_00815"
     CDS             <1..266
                     /locus_tag="VIS73_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761726.1"
                     /translation="VVVAPEVEPPPPPPKAPPLPFKHLGRIVEAGRSLPMHYLMDGDR
                     MLIVSEGDLVGGKYRFVGQIGNQLQFIYLPLNERQNLPMSGDQ"
     gene            416..2548
                     /locus_tag="VIS73_00820"
     CDS             416..2548
                     /locus_tag="VIS73_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEY5761727.1"
                     /translation="MNDNPDDPELRIQYHRRRERTLHRVLDGADAELAGGRPAVAEQI
                     YRDVLAVDSTHARALAGLHSIEMAGRHDALLAEARAALEKDDTATAAQNVKTILTENL
                     NHVEAKALDRELREREQRRSMTPPVLKSRLQNAVNLDFRDAPLKLVIEAISRSSGINF
                     VFDRDVRPDLRTTIVARDVRVENAIDMILAQNQLEKKVISENAVLVYPSTPQKLRENQ
                     ELVMRVFHLGNADVKQTVNLLRTMIKTKDIFVDERMNMLVIRDTPDAIRIAEKLIGMH
                     DMVDPEVVLEIEILEVSRTKVRELGITYPNTFSGPGGTLAQIGDLTRSTVGANSGFAV
                     TALRTDGDTKTLANPRVRVRNKEKARVHVGDRVPVISSTLVGAGSGGAPVSSEQIQYL
                     DVGIKVEAEPVVHPDNTVAVKINLDVSSLGAQTRTTLGTVAYEVGTRNVNTVLRLRDG
                     ETQALMGLIRDDDVQTRSGIPFLGELPILDIIFGNSKTDLRSRELVLLITPHIIRGNT
                     PPDATLAEFWSGTEARMRAEGPFSSDKPVAASPGVKVDTVPVLGNAPAETLRAPEGSD
                     KPASMVLSWSTPEGVQAEKEFAVSLNALGVGNLKAVTVSMRYRPNEVELVGIEDGGFF
                     KRGGGESRFTPRSEPGLGMIVTALGAGASDGSVPGGEGTLLTLKLKPIGKVPKVRIEF
                     ISVVGLDALNRQIPITGMAPLDVPVQQP"
     gene            2526..3026
                     /locus_tag="VIS73_00825"
     CDS             2526..3026
                     /locus_tag="VIS73_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY5761728.1"
                     /translation="MFQYSSRKPRGGARGFTLLELLVTVAIVGTLATVALPLAEVTVK
                     RNREAALMSALREIRSAIDAYKLASDQARIFKVVGETGYPPTLSMLVDGVEDARDPKG
                     ARIYFLRRLPRDPLHPDKTLPPAETWGLRSYDSPPDAPMPGQDVYDVYSLATGVGLNG
                     IPYRQW"
     gene            3035..3409
                     /locus_tag="VIS73_00830"
     CDS             3035..3409
                     /locus_tag="VIS73_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY5761729.1"
                     /translation="MRSARGFTLIELLVVMAIIATLLTIATPRYFDSIRKSEEATLRQ
                     NLSLTRESIDKYFADSGRYPDALEDLVSKRYLRALPFDPIAGNADSWSIVPPPDADLG
                     RVYDLHSSAEGNGRDGTPYASW"
     gene            3403..3984
                     /locus_tag="VIS73_00835"
     CDS             3403..3984
                     /locus_tag="VIS73_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY5761730.1"
                     /translation="MVVTAVAARRQRGAAYLTLMISVAVISAILATTAEIVSHAQQRA
                     REQHLLWVGDQIRRALVSYARFPAGIDTYPKELEDLVNDRRRPLVQNHLRRLYFDPMT
                     GSTDWGVVRNPQQRIVGVFSKSTLTPIKTAGFPTHYNEFEKATSYADWQFVVAAAAQP
                     AAPVAPTPAGQGKPAPILQPLPGSSFAPAPIGR"
     gene            complement(4004..4609)
                     /locus_tag="VIS73_00840"
     CDS             complement(4004..4609)
                     /locus_tag="VIS73_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5761731.1"
                     /translation="MSETADPVRQQHVLLIDDDEVFVSAMARALRGRGFEVTVCTSAA
                     KAKSLIAGIAPDLALVDLRLPDGSGLCLVNELRRYSPDTAVVVLTGYGSIASAVEAMK
                     LGAVNYLCKPVDADTVVAAFGRDDGESVDGAVDKADEGITEAEGDEAVPDQPLTVMRL
                     EWEYINRVLASNDGNISATARALNMHRRTLQRKLLKRPVPR"
     gene            complement(4612..5955)
                     /locus_tag="VIS73_00845"
     CDS             complement(4612..5955)
                     /locus_tag="VIS73_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5761732.1"
                     /translation="MDSSKLTGDSTIHDPARGVVAGAPLLGGADVRLLLRLRAVAIAG
                     QSLALLVSGLLIDIELPFLALSCGVAVLAGLNVYTWLRLRNGARRDELPLHWLDERAF
                     FYQVLVDIAVLTYLLYFAGGPENPFHGMFLLPLTVAAAALPWRYVWRVVLASVTGYTF
                     VALKFVPFTIPPALGGPHEVMMFGQWVNHVLLALLVTYFIVRITQGLRARDRLLEDAH
                     IRELRNSCAVAVGSVAAGVAHELGTPLSTIQVVLREMRRTHADNPAIATDLDVVSDQL
                     GECRSILGGIRDYVQALRNGDKPVPVDTLVDDVAERFLDIRPGVNLSKSLDGPLPAPL
                     IVPDLALQQAMINLLNNAADVSPHAVTVEARWTTSQLVIRVLDRGPGISPALAERLGK
                     VLVSTKAPDAGNGLGLFLTNVTMSRLGGRLKLFNHAEGGACAELVLPVGSAAKPE"
BASE COUNT         1236 a         2177 c         2172 g         1108 t
ORIGIN      
        1 cggtggtcgt cgcgcccgag gtcgagcccc caccgccgcc gccaaaggcg ccgccgttgc
       61 cgttcaagca cctcggccgc atcgttgagg ccggccgctc gctgcccatg cactacctga
      121 tggacggcga tcgcatgctc atcgtttccg aaggcgatct ggtcggcggt aagtaccgct
      181 tcgtcggcca gatcggcaat caactccagt tcatctatct gccgctcaac gagcgtcaga
      241 acttgcccat gtcaggtgac caatgaccca cggacaacgt agaccccttc gccgcctcgc
      301 gttccttatt ctggcagccg ttcttaccgc ctgcgccagc ccggtaatcg aggagagctc
      361 cgagctgatt gacgcgggcc gctggcagga gggtctggcc cggcttgagc gcgggatgaa
      421 tgacaatccc gacgaccccg agctgcgcat ccagtatcac cgccgacgcg agcgcacgct
      481 gcaccgcgtc ctcgatggcg ccgatgctga actcgccggc ggccggccgg cggtcgccga
      541 gcagatctac cgcgacgtgc tggcggttga ctcaacgcac gcgcgggcgc ttgccgggct
      601 gcactcgatc gagatggccg ggcgccacga cgcgttgctc gccgaagcac gcgcggcgct
      661 ggagaaagac gacacggcaa cagcggcgca aaacgtcaag accatcctta ccgagaacct
      721 caaccacgta gaggcgaagg cgctcgaccg cgaactgcgc gaacgagagc agcgccgctc
      781 gatgacgccg ccggtactca agagccgact gcagaacgcc gtcaacctcg acttccgcga
      841 tgcgccgctg aagctggtga tcgaggcgat ttcgcgttca tcgggcatca actttgtttt
      901 tgaccgcgat gtgcgtcccg acctgcggac cacgatcgtc gcccgcgacg tgcgcgtcga
      961 gaacgcgatc gacatgatcc tcgcgcagaa ccaactcgag aaaaaggtga tttcggagaa
     1021 cgccgtgctg gtctacccgt cgacaccgca gaagctgcgc gagaaccagg agctggtgat
     1081 gcgcgtcttc catctcggca acgccgacgt caagcagacg gtcaacttgc tgcggacgat
     1141 gatcaagacc aaggacatct tcgtcgacga gcggatgaac atgctggtca tccgcgatac
     1201 gccggacgcg atccgcatcg ccgagaagct gatcggcatg cacgacatgg tcgaccccga
     1261 agttgtgctg gaaatcgaga ttctcgaagt gtcgcggacc aaggtgcgcg aactgggaat
     1321 tacctatccg aacaccttct ccgggcctgg tggcacgctg gctcagatcg gagacctgac
     1381 gcgcagcacc gtcggtgcca actcgggatt tgccgttacc gcgctgcgca ccgacggtga
     1441 taccaagacg ttggccaacc cgcgcgtgcg agtgcgcaac aaggaaaagg ctcgcgtcca
     1501 cgtcggcgat cgcgtgccgg tgatttcgag cacgttggtc ggcgctggca gcggcggggc
     1561 gccggtttcc tctgagcaga ttcagtatct cgacgtcggc atcaaggtcg aggccgagcc
     1621 tgtcgtccat cctgacaaca cggtggcggt gaagatcaat ctcgacgttt cctcgctcgg
     1681 tgcgcagact cggaccacgc tgggtaccgt ggcctacgag gtcggcacgc gcaacgtcaa
     1741 caccgtgctg cgcctgcgcg atggcgaaac gcaggcgctg atgggcctga ttcgtgacga
     1801 cgacgtgcag acccgctcgg ggattccctt cctcggcgag ctgccaattc tcgacatcat
     1861 cttcggcaat tcgaagaccg acctgcgcag ccgcgaactc gtcctgctga tcaccccgca
     1921 catcatccgc ggcaacacgc cgcccgatgc gacgctggcc gagttctggt cgggaaccga
     1981 agcgcggatg cgcgccgaag ggccgttctc gagcgacaag ccggttgccg cgagccccgg
     2041 cgtcaaggtc gataccgttc ccgtgctggg caacgcacca gccgagacgc tgcgcgcacc
     2101 cgaaggctcg gacaagccgg cgtcgatggt gctgagctgg agcacgcccg agggcgtgca
     2161 ggccgagaag gaattcgcgg tctcgctcaa tgcgctgggc gtcggcaacc tcaaggcggt
     2221 cacggtctcg atgcgttacc gtccgaacga ggttgagctg gtcggcatcg aggatggcgg
     2281 cttcttcaag cgcggcggcg gtgaaagccg cttcacgccg cgcagcgaac cggggttggg
     2341 gatgatcgtc accgcgctcg gcgcgggcgc cagcgacggt tccgtgccgg gcggcgaggg
     2401 taccctgctg acgctcaaac tgaagccaat cggcaaggtc ccgaaggtgc gaatcgagtt
     2461 catctcggtg gtcggactcg acgccttgaa ccggcagatt ccgatcaccg gcatggcgcc
     2521 gctggatgtt ccagtacagc agccgtaagc cacgaggcgg cgcgcgcggc ttcacgctgc
     2581 tcgagctgct ggtgacggtg gccatcgtcg gcacgctggc gacggtggcg ctgccgcttg
     2641 ccgaggtcac cgtcaagcgc aaccgtgagg cggcgctaat gtcggcgctg cgcgagatcc
     2701 gtagcgcgat cgatgcctac aagctggcga gcgaccaggc gcgcatcttc aaggtggtcg
     2761 gcgagaccgg ctacccgccg acgctgtcga tgctggtcga cggtgtggag gacgcgcgtg
     2821 acccgaaggg cgcgcgcatc tacttcctgc gccgcctgcc gcgcgatccg ctgcatccgg
     2881 acaagaccct gccgcccgca gaaacctggg ggctgcgcag ttacgacagt ccacccgacg
     2941 cgccgatgcc cggccaggac gtctacgatg tctattctct ggcgaccggg gtcggcctca
     3001 acggtatccc ataccgccaa tggtgaggac ctccatgcgc agcgcccgcg gctttacgct
     3061 gatcgaactg ctggtggtga tggcaatcat cgccacgctg ctgacgatcg ccacgcctcg
     3121 ctacttcgac agcatccgca agagcgagga ggcgacgctg cggcagaacc tctcgctgac
     3181 ccgggaatcg atcgacaagt acttcgcgga cagtggccgc tatcccgacg cgcttgagga
     3241 tctcgtcagc aagcgctacc tgcgcgcgct gcccttcgat ccgatcgcgg gcaacgccga
     3301 ttcctggagc atcgtgccgc cgccggacgc cgacctcggg cgggtctacg acctgcacag
     3361 cagcgccgag ggcaacggcc gcgatggaac gccttacgcg tcatggtagt cacggccgtc
     3421 gccgcaaggc gccagcgcgg cgccgcctac ctgacactga tgatttccgt cgccgtgatc
     3481 tcggcgatac tcgcaaccac ggccgaaatc gtcagccacg cgcagcagcg tgcgcgcgag
     3541 cagcacctgc tctgggtcgg tgaccagatt cggcgcgcgc tggtttccta tgcgcgcttt
     3601 ccggccggta tcgacaccta tcccaaggaa ctcgaggatc tcgtcaacga ccgtcggcgt
     3661 ccgctggtcc agaaccacct gcgccggctc tacttcgatc cgatgaccgg cagcaccgac
     3721 tggggcgtgg ttcgcaatcc gcagcagcgg atcgtcggcg tcttcagcaa gtcaacgctc
     3781 acaccgatca agaccgcggg tttcccaacg cactacaacg agtttgaaaa ggcaacgagc
     3841 tacgccgact ggcagttcgt cgtcgcggct gcggcgcaac cggcggcccc cgtggcgccg
     3901 acaccggcgg ggcagggaaa accggcgccg atactgcaac cgcttcccgg ctcgtccttc
     3961 gcgccggcgc cgatcggccg ataggccgcg acgctcgccg cggttagcgc ggtaccgggc
     4021 gcttcagcag ttttcgctgc agcgtgcgcc ggtgcatatt gagcgcgcgt gcggtcgctg
     4081 agatgttgcc gtcgttggag gcaagaacgc ggttgatgta ttcccactcg agtcgcatca
     4141 cggtcagagg ctgatcggga accgcctcat cgccctcggc ctcggtgatg ccctcgtcgg
     4201 ccttgtctac cgccccatcg accgactcgc catcgtctct gccgaaggcg gcaaccacgg
     4261 tatcggcatc gaccggcttg caaaggtagt tgacggcgcc cagtttcatc gcctcgaccg
     4321 ccgaagcgat gctgccgtag ccggtcagca cgacgacggc ggtatcgggg ctgtaccggc
     4381 gcaactcgtt gaccagacag aggcccgaac catcgggcag gcgcaggtcg accagcgcga
     4441 gatcgggggc gatgccggca atcagggact tggccttcgc cgccgaggtg cacaccgtga
     4501 cctcgaagcc gcggccacgc agcgcgcgcg ccatcgcact gacaaacact tcgtcgtcgt
     4561 cgatcagcag cacgtgctgt tgtctcacgg gatcggcagt ctcgctcatg gctactccgg
     4621 cttcgcggcg ctaccaacgg gcagcaccaa ctcggcgcag gcgcctccct cggcatggtt
     4681 gaaaagtttg agtcggcccc cgagccgact catcgttacg ttagtcagaa aaagtccgag
     4741 accgttcccg gcgtcgggcg ccttggtgct aacgaggacc ttgcccagcc tctcagcaag
     4801 cgccggcgag atgccagggc cgcgatcaag aacgcggatc accagctggc tcgtcgtcca
     4861 ccgggcctcg acggtcaccg cgtgtggcga gacatcggcg gcgttgttga gcagattgat
     4921 catcgcctgt tgcaacgcca ggtcgggaac gatcaatggg gccggcagcg gaccgtcaag
     4981 cgacttcgac aggttgacgc cggggcggat gtcgaggaag cgctcggcga catcgtctac
     5041 cagagtgtcg accgggactg gcttgtcgcc gttgcgcaac gcttgcacgt agtcgcggat
     5101 accgccgagg atgctccggc attcgccgag ctgatcggag acgacgtcga ggtcggtggc
     5161 gattgccggg ttgtcggcgt gggttcggcg catctcgcga agcacgacct ggatcgtcga
     5221 cagcggagtc cccagttcgt gcgcaacacc ggccgccacc gagccgacgg cgacggcgca
     5281 gctgttgcgc agttcgcgga tgtgggcgtc ctccagcagc cggtcgcggg cgcgcagccc
     5341 ctgcgtgatt cggacgatga agtaggtcac cagcagcgcc agcagcacat ggttcaccca
     5401 ctggccgaac atcatcactt catgaggtcc gccgagcgcc ggtggaatcg tgaagggaac
     5461 gaacttcaat gcgacaaagg tgtacccggt gacgctggcg agcaccactc gccagacgta
     5521 gcgccagggc agcgccgccg cggcgaccgt cagcggcagc aggaacatgc cgtggaaggg
     5581 attctccggc ccgccggcaa aatagagcag gtaggtgagt acggcaatgt cgacgaggac
     5641 ttggtagaaa aaggcgcgct cgtcgagcca gtgcagcggc agttcgtcgc gccgcgcacc
     5701 atttcgcagg cgaagccacg tatagacgtt gaggccggcg agcacggcca cgccgcagct
     5761 cagcgcgagg aagggcagct cgatgtcgat cagcagcccg ctgacgagca gcgccaacga
     5821 ctgcccggcg atcgccactg cgcgcaggcg caacagcagg cgtacatcgg cgccgccgag
     5881 cagcggcgcg ccggcaacga caccgcgcgc cgggtcgtga atcgtgctgt caccagtcag
     5941 cttagaggaa tccaaagcgt ctcctgtcgg gcaattaaaa gggccgcgtt acgcccgggg
     6001 gcgggggcat ggcggctgct tcgagtggtc cgggtttttg cgggcacgaa ttcttctggg
     6061 ttggtttcag tgcaggcggc gatgcatccg gcacagatcg cacagcagca ggcatgccac
     6121 ttccgtcgaa gcacctgcca atcgcggcgc aagaccgctg gcatggccgg tgccggcagg
     6181 tcgcgatccg aggatcagtg gatcgtgcgg tcattccgat tccgggatgt gccaactagt
     6241 gcacgtgcat gtgccgaaag aaggcgcaac tccaaacggt ctgttcagtt tgcatggtgc
     6301 catcagccgt tttggagtgg ggaaaatcta aaaaatcccc actcgaattc cccaatcagt
     6361 gggtaaaccg gcggtgaatc ttcttcgccg cgaaccgatc cgcttctccg atgcgccgca
     6421 ctgagcggcg atttcccgtg ggttggggaa gttggcccgg atgctgcaac tacagccggc
     6481 agggagtcgt tttcggaggg gtgcgacaag ttgtcgcgcg actctgtgac acattcccag
     6541 agcataaggc agtccgtagc atggcttcgt cggtaagtgt cccaagtagc gcagtgcggt
     6601 tcgcggggtc tcctaccggt tgtcttttaa ccgcagtcac ttctctcaat gggaggcaaa
     6661 ccatgaagaa atggagacca cccggttccg gcg
//
LOCUS       DATDAA010000024        10583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_106831,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000024 DATDAA010000000
VERSION     DATDAA010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10583
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106831"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..1065)
                     /locus_tag="VIS73_00850"
     CDS             complement(295..1065)
                     /locus_tag="VIS73_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY5761733.1"
                     /translation="MSTLAWIVASCFAGGLLSVIGAALLGRTARDGAVHILVSFAIGA
                     MLGAVFLEVLPHAIENSKDIHALAGTVLAGILVFFVLEKLVLWRHCHAHHCEAHEALP
                     KDHDHGRSGLLVLVGDTVHNFVDGILIAAAFMQDTQLGIVTAFAIVVHEIPQELGDFM
                     VLLHSGYTRRRALIFNALSGLATVVGGVLAYYALSSLQEWIPSLLAFAAASMIYVAVA
                     DLIPGLHKRTALGETLQQVILIALGIFAVWGVGELLGH"
     gene            complement(1112..1855)
                     /gene="pgeF"
                     /locus_tag="VIS73_00855"
     CDS             complement(1112..1855)
                     /gene="pgeF"
                     /locus_tag="VIS73_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY5761734.1"
                     /translation="MATDPGFIRPDWPAPAGVEALSTTRAGGVSSGVYASLNLGDHVG
                     DDPSAVIANRRLVASVVPGTPVWLEQIHGCEVAEIDRAGTIPPIDAAVCRTPGKACVI
                     MTADCLPVLFCDRFGTVVGAAHAGWRGLLAGVLENTVTAMGVPPAHILAWFGPAIGPS
                     AFEVGEEVRAAFVSRNAAAASAFVANERGRWLADLYLLARQRLAACGVVDVSGGDRCT
                     FSEPDCFFSYRRDGRTGRMATMVWLTDKR"
     gene            complement(1845..2855)
                     /gene="rluD"
                     /locus_tag="VIS73_00860"
     CDS             complement(1845..2855)
                     /gene="rluD"
                     /locus_tag="VIS73_00860"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEY5761735.1"
                     /translation="MMKIQTKEAADYSGAPDPAVDGDDDDWSGEAAGKAAVPERELVT
                     LPLAAGGERLDLALAAVLGQYSRSRLQAWIRAGRVTVNDKACTDVRQIVRGGETVGVA
                     IEADPATQAALAQPVAFPIVYEDESLIVINKPAGLVVHPGSGNWDGTLLNGLLHHDSQ
                     LAMLPRAGIVHRLDKDTTGLMVVARTLLAQTDLVRQLQARGVKRDYLAVVKGELARSG
                     SVDAPIGRHPTQRVRMAVVATGKPAVTHYRPLEALRGATLVGCSLETGRTHQIRVHLA
                     HLGHPLLGDPLYGGPRNAFPRQALHATRLGLIHPASGREMKWEAALPEDIVALLDTLR
                     GD"
     gene            2854..3684
                     /locus_tag="VIS73_00865"
     CDS             2854..3684
                     /locus_tag="VIS73_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY5761736.1"
                     /translation="MRSLPALNGGQPARRALRALALALVTTLVVACSTKGPFDETARW
                     TAEELYEEAKTAQEDGIFDKAIKYFERLEARFPFGRYAQQAQIEIAYTHYKAGDPGDA
                     VAACDRFLRLFPDHEHADYVYYLKGLASFSGNLGLFADLGGQDPTERDPKGMRDAFDA
                     YKTLLQRFPQSRYAADAQQRSAWLVNALASHEVHVARYYLSRTAYVAAVNRAQDVVRS
                     YPQTPAIENALAVMVAAYDRLGMTDLRDDALAVMRLNFPNSDYYVLPEEYTGPWWARL
                     "
     gene            3681..3920
                     /locus_tag="VIS73_00870"
     CDS             3681..3920
                     /locus_tag="VIS73_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761737.1"
                     /translation="MSEARGVLGWIDRLPWLPLLVIAVLLGLAPFSPEPHLVEKLRML
                     VSGTLSRPLDIFDLLMHASPLAVVAVKLARRRLAR"
     gene            complement(3917..4474)
                     /locus_tag="VIS73_00875"
     CDS             complement(3917..4474)
                     /locus_tag="VIS73_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY5761738.1"
                     /translation="MRVPIREEHEYPFWLRPFFASQRRHYGKPLTSGQVWARVPRLFA
                     AIALLYGALDRKRSPVAPVLRSLIMVRVSQINWCEFCVDLNGATLAKRAGSFEKVEAL
                     EGWRDSPLFDARERAVLDYAEAVTYSDRQVSEEMMIELRRYFDDDGIVELTGLIAFQN
                     LSSKFNAALDIPAQGFCKLPTRPKA"
     gene            complement(4501..6438)
                     /locus_tag="VIS73_00880"
     CDS             complement(4501..6438)
                     /locus_tag="VIS73_00880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY5761739.1"
                     /translation="MSDSSLILAIDAAFAEGGPLSEAVSGYRARAQQVEMAQAIAEAI
                     AGNSQLVVEAGTGTGKTFAYLVPALLSGGKVILSTGTKNLQDQLFTKDLPAVREALRL
                     PVSVALLKGRANYVCHHHLERAVTDGRFASKEEVVQMRRLQDFARHSDSGDRGECIDV
                     PESSPVWARVTSSRENCLGQECAFVKDCFVLAARRAALAADLVVVNHHLFFADVLLRD
                     EGVAELLPACNTVIFDEAHQLPETARLFFGESLSSAQAIDLARDARNEGLANASDCRA
                     LQDACRELDKSARDFRLTVARDVGRLPRSELVSATGFTEALQSLRDKLVAIAAVLETQ
                     AERAEALAQCAGRAAELLDRIDGWLDDTSADWVRWGECFSHSLVLHRTPLDVAEIFRR
                     QLQGLPRAWIFTSATLSVHGSFDHYCGELGLGEARTVSWPSPFDYPAQSLLYVPTDMP
                     EPNQPDYSEAVVRAVWPVIRASGGRAFLLCTSLRAMRRIHELVGERIAEARLDMPLLL
                     QGEGSKNDLLDRFRRLGNAVLIGSQSFWEGVDVRGEALSLVVIDKLPFVPPDDPVLAA
                     RIEQLRRAGRNAFMEYQLPHAVISVKQGAGRLIRDEADRGVLVICDPRLIDKPYGRRI
                     WQSLPPMRRTRREDEAVAFFT"
     gene            6587..7639
                     /locus_tag="VIS73_00885"
     CDS             6587..7639
                     /locus_tag="VIS73_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761740.1"
                     /translation="MRAIALIALLAILSEVAHAQAPVASCANTPLPARCLLDLAQRES
                     QRVTAPSARYQALAKLLNAAATAALSLPVTAESAARAIEANFLDDANRLDLLMALQNY
                     RTRFPESGAETRLAALALYLGLAENGVGSARLSLMLTACGVLELPEPLATQWQPMVDH
                     GCTPAFLDETTLPGDTDRLMGILLAPIVFYREDEAEAFEAAVDRAHATVRDMDGQMED
                     KPDATRAAWQQLKAGMYLLHGASFADFGDLLRTRLSAAASYAAVLAAEEIGGIVLAEE
                     RASVAALLMRAEQRSTAEQILDETTQLIDDDPGHQRVPAAGRVGFLTALVELLAGEAE
                     SLFCPEPDAADSQPAV"
     gene            complement(7643..8191)
                     /locus_tag="VIS73_00890"
     CDS             complement(7643..8191)
                     /locus_tag="VIS73_00890"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY5761741.1"
                     /translation="MDDLTEHLTSEEQMAKGRLLDARRATVALPDGTSGTREYVVHPG
                     AVLIIPRLDDGRLLFERQHRFPIRQTILELPAGKIDPGEAIADTATRELLEETGHTAE
                     RWRYLGVIHPCVGYSNERIEIFLAEGLSRVSTPQLDAGEFLEILPMGVEECREAVRDG
                     RITDGKTIAALFWLDGVDRYGW"
     gene            complement(8258..9841)
                     /gene="nuoN"
                     /locus_tag="VIS73_00895"
     CDS             complement(8258..9841)
                     /gene="nuoN"
                     /locus_tag="VIS73_00895"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEY5761742.1"
                     /translation="MDFLMPDLAAASAEIFVLVMACAILVIDVFLKDPKRTLSYVLTQ
                     ATLIGGAIIVLGTSAFALGLAQSEFQPFALIGGLLKFIFNPAESRVLEVAVAFNGTYV
                     DDLMGDLLKIAVLIAVSATLFYSRGYQLDRPALARGEFFVLALFATLGMMVMISANHF
                     LTIYLGLELLSLSLYAMVALNRDSPVSNEAAMKYFVLGAIASGMLLYGMSMIYGATGS
                     LEIGKVAEALYVQSANRSIMVFGVVFLVAGIAFKLGAVPFHMWVPDVYHGAPTPVTML
                     IGGAPKLAAFALIIRLLVNGLITEASPLEGGADWQSMLVILSVLSMAIGNLAAIAQTN
                     LKRMLAYSAISHMGFMLLGMATGVVQGDVRYALNAYSSSMFYMITYVIMSIGAFGMIL
                     LMARAGFESDEIEDFKGLNKRSPWFAAMMMVLMLSMAGIPFFVGFFAKFSVLQAVVAA
                     EMYWLAVVAVIFSLFGAYYYLRIVKLMYFDAPKDETPIEASLDMRVLMSINGLAVIGF
                     GLMPQTLMSLCAFALLRSL"
     gene            complement(9873..>10583)
                     /locus_tag="VIS73_00900"
     CDS             complement(9873..>10583)
                     /locus_tag="VIS73_00900"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY5761743.1"
                     /translation="YGFVRFTLPILPDASRELAWFMIALSLIAVVYIGLVALVQADMK
                     KLIAYSSISHMGFVTLGFFIFNAIGIEGALIQMISHGFVSGAMFLCIGVMYDRMHSRN
                     IADYGGVVNTMPKFAALMMLFAMANAGLPATSGFVGEFMVILGAVQFNFWIAVIAATT
                     MIVGAAYTLWMYKRVIFGAVANPHVAELTDLEGREFFVLMVLAVAVLLMGLYPAPFTD
                     VMHTSVNELLRHVAQSKL"
BASE COUNT         2038 a         3499 c         3349 g         1697 t
ORIGIN      
        1 gctcgaagta gctgtctgcg gaatactcga ccttgggtcc gtcccaagcg actgccgaac
       61 ccgccaaagc cgaagctgcg accactgcaa tcaatgtttt gatatatgcc atatgcttcc
      121 ccttgtcgtt cggcccatgc cttaagcctc taagacgacc aaatagccgt ccatatgccg
      181 tagaaatcta gcctaggcaa tcttttcttt ccagtgaatt tgttggcgcg gtatcgacta
      241 tcgcgtggaa cgaatccgcc gtccgaagct tccagcccat cggacctaac aggactagtg
      301 gccgagcagc tcgccgacac cccacacggc gaagatgccg agggcgatca gaatcacctg
      361 ctgcagcgtc tcgccgagcg ccgtgcgttt gtgcagaccc gggatcaggt cggcgaccgc
      421 gacgtagatc atgctcgcgg ccgcgaaggc tagcagcgag ggaatccact cctgcaggct
      481 cgacagcgcg tagtacgcga gaaccccgcc gaccacggtc gcgaggccgg agagcgcgtt
      541 gaagataagc gcgcgccgcc gcgtatagcc cgaatgcagc aggaccatga agtcgccgag
      601 ctcctgcgga atctcgtgga cgacgatggc gaaggcagtg acgatgccga gctgggtgtc
      661 ctgcatgaag gccgcggcga tcagaatgcc gtcgacgaag ttgtggacag tgtcgccgac
      721 cagcaccaag aggccgcttc gaccgtggtc gtggtccttg ggcagcgcct cgtgggcttc
      781 gcaatggtgc gcgtgacaat ggcgccagag caccagcttc tcaaggacga agaagaccag
      841 gatgcccgcg agcacggtgc cggccagcgc gtggatatct ttcgaattct cgatcgcgtg
      901 cggcagaacc tccagaaaca ccgcgccgag catggcgcca atggcgaagg aaaccaatat
      961 gtggacggca ccgtcacgcg cggtacggcc gagcaaagcc gcgccgatga cgcttaacaa
     1021 gccgccggcg aaacaagaag cgacgatcca ggccaatgta ctcatgggtt cctcgggcag
     1081 gtcgggggcg gattatgccc ctgccggcgc gctagcgctt gtcagtcagc cacaccatcg
     1141 tcgccatgcg gccggtcctg ccgtcgcggc ggtaggaaaa aaagcagtcg ggctcggaga
     1201 aagtgcagcg gtcgccgccg ctgacatcga cgacgccgca cgccgcaagc cgctgccgcg
     1261 cgagcaagta gagatcggcc agccagcgtc cgcgttcatt ggcgacgaac gcggaggctg
     1321 ccgccgcgtt gcgggaaacg aaggccgcgc gcacttcttc gccgacctcg aaggccgacg
     1381 ggccgatcgc cgggccaaac caggcgagaa tgtgcgccgg cggcacgccc attgccgtca
     1441 ccgtattttc aagaaccccg gccagcagtc cgcgccagcc agcgtgggcg gcaccgacga
     1501 cggtgccgaa gcggtcgcag aacagcaccg gcagacagtc ggcggtcatg atcacgcaag
     1561 cctttcccgg cgtgcgacag acggcggcat cgatcggcgg aatcgtcccg gcacggtcga
     1621 tctcggcgac ctcgcagccg tgaatctgct cgagccatac aggcgtgcca ggcaccacgc
     1681 tggccacgag acgccggttc gcgatgacag ctgatggatc gtcaccaacg tgatcgccga
     1741 gattgaggct ggcatagacg ccgctgctga cgccgccggc ccgcgtggtc gacagcgcct
     1801 cgacgccggc cggtgccggc cagtcggggc ggatgaaacc gggatcagtc gccacgcagg
     1861 gtatccagca gcgcgacgat gtcctcggga agcgccgcct cccacttcat ttcgcgaccg
     1921 ctcgccggat ggatcaggcc cagccgcgtc gcgtgcagtg cttggcgcgg gaaggcattg
     1981 cgcggcccgc cgtagagcgg atcaccgaga agtggatgac cgaggtgcgc aaggtgaaca
     2041 cgaatctggt gcgtacggcc ggtttccagc gaacagccca ccagcgtcgc cccgcgcagt
     2101 gcttcgagcg gccgatagtg cgtcactgcc ggcttgccgg tggcgaccac ggccatgcgc
     2161 acgcgctgcg tgggatgacg cccaatcggg gcgtcgacgc tgcccgaccg ggccagctcg
     2221 cccttcacga ccgccaaata gtcacgcttg acgccccgtg cctgcagctg gcggacgagg
     2281 tcggtctgcg cgagcagcgt ccgggcgacg accatgagcc ccgtggtgtc cttgtcgagc
     2341 cggtggacaa tgccggcgcg cggcagcatc gcgagctgcg aatcgtggtg caggagcccg
     2401 ttaagcagcg tgccgtccca gttgccgctg cccggatgga cgaccagacc agccggcttg
     2461 ttgatgacga tcagcgactc gtcttcgtag acgataggaa acgccaccgg ctgcgccagt
     2521 gcagcctgcg tcgcgggatc ggcctcgatg gcaacgccaa cggtttcgcc gccgcggacg
     2581 atctggcgca cgtcggtgca tgccttgtca ttgacggtca cgcgcccggc gcggatccac
     2641 gcctgcaagc ggctgcgcga gtactggcca agcaccgcgg cgagcgctag gtcaagcctt
     2701 tccccgcccg cagccagcgg cagcgtgacg agctcgcgct cgggtacggc ggccttgccc
     2761 gccgcttcgc cactccagtc atcgtcatcg ccatcgacgg cgggatcggg ggctccgcta
     2821 taatcggcgg cctctttggt ctggattttc atcatgcgca gtttaccggc attgaacggt
     2881 ggccagccgg cgcgtcgggc gcttcgcgcc ctcgccctcg ccctcgtcac caccctcgtc
     2941 gtcgcctgtt cgaccaaggg cccattcgac gaaaccgcac gctggaccgc cgaagaactg
     3001 tacgaagaag ccaagaccgc acaggaagac ggcatcttcg acaaggcgat caagtacttc
     3061 gagcgcctcg aagcgcgctt ccccttcggc cgctacgcgc aacaggcgca gatcgagatc
     3121 gcgtacacgc actacaaggc tggcgacccc ggcgacgccg ttgccgcctg cgaccgcttc
     3181 ctgcgcctct tccccgacca cgagcacgcc gactacgtct attacctgaa gggtctggcg
     3241 agcttcagcg gcaacctcgg cctgtttgcc gacctcggcg gccaagatcc gaccgagcgc
     3301 gacccaaagg gaatgcgcga cgccttcgat gcctacaaga cgctgctgca gcgcttcccg
     3361 cagagccgct acgctgccga tgcgcagcag cgctcggctt ggctggtcaa cgcactggct
     3421 tcgcatgaag tccatgtcgc gcgctactac ctctcgcgca ccgcctacgt cgccgccgtc
     3481 aaccgcgccc aggatgtcgt gcgtagctac ccgcaaacgc cggcaatcga aaacgcgctg
     3541 gcggtcatgg tcgccgccta cgaccggctt ggcatgaccg atctgcgcga cgacgccttg
     3601 gcggtgatgc gccttaactt ccccaacagt gattactacg tgctccccga ggagtacacg
     3661 ggtccgtggt gggcacgcct gtgagcgaag cccgcggcgt actcggctgg atcgaccgcc
     3721 tcccttggct gccgctgctg gtgatcgcgg tgctgctggg tctcgcgcct ttttcgcccg
     3781 aaccgcatct ggtcgagaag ctgcgcatgc ttgtcagtgg aaccttgtcg cggccgctcg
     3841 atatcttcga cctgctcatg cacgcctctc cgctggcagt ggtcgccgtc aagctggccc
     3901 gccgccggct ggcacgctag gccttcggcc gcgtcggcag cttgcagaat ccctgcgccg
     3961 ggatgtcgag cgccgcgttg aacttgctcg acagattctg gaaggcgatc agtccggtca
     4021 gctcgacgat gccgtcgtcg tcgaagtatc gccgcaactc gatcatcatt tcctcgctga
     4081 cctgccggtc cgaataagtc accgcctcgg cgtaatcgag aaccgcgcgc tcgcgcgcgt
     4141 cgaacagcgg actgtcgcgc cagccctcca gcgcctcgac tttttcgaat gagccagcgc
     4201 gtttggccag cgtcgcgcca ttaaggtcga cacagaactc gcaccaattg atctgtgaaa
     4261 cacgaaccat gatcaggctg cgcagcaccg gggcgaccgg cgaacgcttg cggtcgagcg
     4321 ccccgtagag cagcgcgatc gccgcaaaca gtcgcggcac gcgcgcccag acctgacctg
     4381 aggtcagcgg cttaccgtag tggcgacgtt ggctggcgaa gaagggtcgc aaccagaacg
     4441 ggtattcgtg ttcctcgcgg atcggtacgc gcatgaaggt ctccgggtga ctttctggaa
     4501 tcaggtaaag aaggcgaccg cctcgtcttc gcgccgcgtg cggcgcatcg gcggcaggct
     4561 ctgccagatg cggcggccgt aaggcttgtc aatcagtcgc gggtcgcaga tcaccagtac
     4621 accgcgatcg gcctcgtcgc ggatcaagcg gccggcgccc tgcttgacgc tgatcaccgc
     4681 gtgcggcaac tgatactcca tgaaggcgtt gcggccggca cggcgcagct gctcgattcg
     4741 cgctgcgaga accggatcgt cgggcggcac gaagggtagc ttgtcgatga ccaccagcga
     4801 cagcgcctcg ccgcgcacgt cgacgccctc ccagaagctc tggctgccga tcagcacggc
     4861 attgccgagc cggcggaagc gatcgagcag gtcgttcttc gagccttcac cttgcaacag
     4921 tagcggcatg tcgaggcgtg cctccgcaat ccgctcgccg accagttcgt gtatgcgacg
     4981 catcgcgcgc agactggtgc agagtaggaa agcccggccg ccactggccc ggatcaccgg
     5041 ccagacggcg cgtaccaccg cttccgaata gtcgggctga tttggctccg gcatgtcggt
     5101 gggcacgtag agcaggctct gcgccgggta gtcgaaggga ctcggccagg acacggtgcg
     5161 cgcctcgccc aaacccagtt cgccgcagta atggtcgaag ctgccatgca ccgagagcgt
     5221 cgccgacgta aatatccaag cgcgcggtaa accctgcagc tggcggcgga agatctcggc
     5281 gacgtccagc ggcgtacggt gcaggaccag agaatggctg aagcattcac cccagcgcac
     5341 ccagtcggcg ctggtgtcgt cgagccagcc atcgatgcgg tcgagcagct cggcggcgcg
     5401 cccggcgcac tgcgccaagg cctcggcgcg ctcggcctgt gtctctagca cggcagcgat
     5461 ggcgacgagt ttgtcacgca agctttgcag cgcctcggtg aatcccgtcg cgctcaccag
     5521 ttcggatcgc ggcaggcggc cgacatcgcg ggccaccgtc aggcggaagt cgcgcgcgga
     5581 cttgtcgagt tcgcgacagg cgtcctgcag cgcacgacag tcgctggcgt tggcgagacc
     5641 ctcgttgcgc gcatctcgcg ccagatctat tgcctgcgcc gaagacaggc tttcgccgaa
     5701 gaacagccgc gcggtttcgg gcaactgatg cgcctcgtcg aagatcaccg tattgcacgc
     5761 cggcagcaat tcggcaacgc cttcgtcgcg cagcagcacg tcggcaaaga acaggtgatg
     5821 gttgactact accaggtcgg cagcgagcgc cgcgcgccgt gccgcgagaa cgaaacagtc
     5881 cttgacgaag gcgcactcct gcccgaggca gttctcgcgc gacgaggtaa cgcgggccca
     5941 gaccggcgag ctttccggca cgtcgatgca ttcgccgcgg tcgccgctat cgctgtgccg
     6001 cgcgaaatcc tgcaggcggc gcatctgcac cacctcctcc tttgacgcga agcggccatc
     6061 ggtcactgcg cgttcgaggt gatggtggca gacgtaattg gcacgcccct tgagcaaggc
     6121 gacgctgacc ggcagccgca gcgcctcgcg taccgcaggc aaatccttgg tgaacagctg
     6181 gtcctggaga ttcttggtgc ccgtcgacag gatcaccttg cccccggaga gcagcgccgg
     6241 cactaggtag gcgaaggtct tgccggtgcc cgttccggcc tcgaccacga gctgggaatt
     6301 accagcgatt gcctcggcga tggcttgcgc catctcgacc tgctgcgcac gcgcgcggta
     6361 gccgctgaca gcctcgctca gcggcccgcc ttcggcaaag gccgcatcga tggcgaggat
     6421 cagggaactg tcggacacag ggggcttggc agcaactcgg atgggcgggc gaacagacgc
     6481 gaccgcaggc cggattctac ctgcgcagcg ctggcgtccc cggctcaatt gcgggcgtca
     6541 cgccgcccca tgccataatc aacgggttcc tacccttccg ccgcccatgc gcgcaattgc
     6601 cctgatcgct ctgctggcga tcctttctga ggtcgcacac gcgcaggcgc cagttgctag
     6661 ctgcgccaac acgccactac ccgcccgttg cctgctcgac ctcgcgcaac gcgagagcca
     6721 gcgtgtcacc gcaccgtcgg cgcgctacca ggcactcgcc aaattgctca atgccgcggc
     6781 aacggcggca ctgagcctcc cggtcacggc cgaatcggct gcccgcgcaa tcgaggcgaa
     6841 cttcctcgac gacgccaatc ggctcgatct cctgatggcg ctgcagaact accggacgcg
     6901 cttccccgaa agcggcgcgg aaacgcggct cgccgcgctc gcgctgtatc tcggcctcgc
     6961 cgaaaacggg gttggctccg cccgcctctc gttgatgctg accgcctgcg gcgtgctcga
     7021 attgcccgaa ccgctggcca cgcaatggca gccgatggtc gatcacgggt gcacgccggc
     7081 cttcctagat gaaaccacgc tccccggcga taccgaccga ctgatgggca tcctgctggc
     7141 gccgatcgtc ttctatcgcg aggacgaggc cgaggccttc gaggcagcgg ttgatcgcgc
     7201 ccacgcaacg gtgcgcgaca tggacgggca aatggaggac aagccggacg cgacgcgcgc
     7261 tgcctggcag cagctcaagg ccgggatgta cctgctgcac ggcgcgagct tcgccgattt
     7321 cggcgacctg cttcggaccc gtctgtcggc cgcggcctcc tacgccgccg tgctggccgc
     7381 cgaggaaatc ggcggaatcg tcctcgccga agagcgtgcc tcggtcgcgg cgctgctgat
     7441 gcgtgccgaa cagcgctcga cggccgaaca gatcctcgac gaaaccacgc agctgatcga
     7501 cgacgatccg ggccaccaac gcgtgccggc cgctggccgc gtcggcttcc tcacggcgct
     7561 ggtcgagctg ctcgccgggg aagccgaatc gctgttctgc ccagagcctg acgccgccga
     7621 ttcgcagccg gcggtctgag tcctaccagc cgtagcggtc gacgccgtcg agccagaaca
     7681 gcgcggcaat ggtcttgccg tcggtgatcc gcccatcgcg taccgcttcc cgacattcct
     7741 cgacgcccat cggcaggatt tcgagaaact cgccggcgtc gagctggggt gtagacacgc
     7801 ggctcagccc ttcggcgaga aatatctcga ttcgctcgtt cgaatagcct acgcaggggt
     7861 ggatcacccc gaggtaacgc caacgttcgg cggtatgccc ggtctcctcc agcagctcgc
     7921 gggtcgccgt atcggcgata gcctcgcccg gatcgatctt gccggccggc aattcgagga
     7981 tcgtctgccg gatcggaaag cgatgctgcc gctcgaacag cagtcgacca tcgtcaaggc
     8041 gcgggatgat cagcacggca ccaggatgaa cgacgtattc gcgcgtgccg ctggtcccgt
     8101 cgggaagcgc gacggtggca cgacgggcgt cgagcagccg acccttggcc atctgctctt
     8161 cgctggtgag gtgttcggtc agatcgtcca cggcggtagt ctccggaaaa gcaaaaggcc
     8221 cgacagcaat gtcgggcctt tcatcagcca attcgcgtca gagcgaacgt agcagtgcga
     8281 atgcgcagag cgacatcagc gtctgcggca tcaggccgaa gccgatcacc gccagaccgt
     8341 tgatcgacat cagcacccgc atgtcgagcg aggcctcgat cggcgtctcg tccttcggcg
     8401 catcgaagta cattagcttg acgatgcgca ggtagtagta cgcgccgaac agggagaaaa
     8461 tcaccgcaac gaccgccaac cagtacatct cagcggcgac aactgcctgc aacaccgaga
     8521 acttggcgaa gaagccgacg aagaacggaa tgccggccat cgacagcatc agaaccatca
     8581 tcatggcggc gaaccagggg ctgcgtttgt tcaggccctt gaagtcctcg atctcgtccg
     8641 attcgaagcc ggcacgcgcc atcagcagga tcatgccgaa ggctccgatc gacatgatca
     8701 cgtaggtgat catgtaaaac attgacgagc tgtaggcatt gagcgcgtaa cgaacgtcac
     8761 cctggacgac gccggtggcc atgccaagca gcatgaagcc catgtgtgag attgccgagt
     8821 aggcaagcat gcgtttgagg ttggtctgcg cgatcgcggc gaggttgccg atcgccatcg
     8881 acagcaccga aagaatcacc agcatgcttt gccagtcggc accaccttcg aggggtgacg
     8941 cctcggtgat caagccgttg accaacagcc ggatgatcag cgcgaaggct gcgagcttgg
     9001 gcgcgccacc gatgagcatg gtcaccgggg tcggggcgcc atgatagacg tccggcaccc
     9061 acatatggaa cggcacggcg ccgagcttga aggcgatgcc ggcgaccagg aagacgacgc
     9121 cgaagaccat gatcgacctg ttcgccgact ggacgtagag ggcctcggca accttgccga
     9181 tctccaggct accggttgcg ccgtagatca tcgacatgcc atagagcagc atgccggagg
     9241 caatcgcacc gaggacgaag tacttcatcg ccgcctcgtt cgacaccggc gaatcgcggt
     9301 ttagcgcaac catggcgtag agcgacagcg agagcagttc gagaccgagg tagatggtca
     9361 ggaagtgatt cgccgagatc atcaccatca tccccagcgt cgcaaacaag gcgaggacga
     9421 agaactcacc gcgcgccagc gccggccggt ctagctgata gccgcgcgaa tagaacaacg
     9481 tcgccgaaac ggcaatcagc accgcgatct tcagcaggtc gcccatcagg tcgtcgacat
     9541 aggtgccatt aaaggcaacc gccacctcga gcacccgcga ctcggcgggg ttgaagatga
     9601 acttgaggag cccaccgatc agcgcgaacg gctggaactc agactgggca aggccaagcg
     9661 caaaggcgct ggtgcccagc acaatgatgg caccgccaat cagggtcgcc tgtgtcagca
     9721 cgtagctcaa ggttcgcttg ggatccttga ggaacacgtc gatcaccaga atcgcgcagg
     9781 ccatcaccag cacgaagatt tcggcggaag ctgccgccag gtcaggcatc agaaaatcca
     9841 tcttggaacc ttccagtgtc cgcgcgcgga cgtcagagct tgctttgcgc gacgtggcgc
     9901 aggagttcgt tgaccgaggt gtgcatcacg tcagtgaagg gcgccggata caggcccatc
     9961 aacaggactg cgacagccag caccatcagc acgaagaatt cgcgcccctc gagatcggtc
    10021 agttcggcga cgtgcgggtt ggctaccgca ccgaaaatca cacgcttgta catccacaac
    10081 gtgtaggcgg cgccgacgat catcgtcgtc gcggcaatga cggcaatcca gaagttgaac
    10141 tgtacggcgc caaggatgac catgaactca ccgacaaagc ccgaggttgc cggcaggccg
    10201 gcgttagcca tcgcaaacag catcatcagt gccgcaaact tgggcatggt gttcacgacg
    10261 cccccgtaat cggcgatgtt gcgcgaatgc atgcggtcgt acatgacgcc gatgcacaag
    10321 aacatcgcac cggagacaaa gccgtgcgaa atcatctgga tcagcgcgcc ctcgataccg
    10381 attgcgttga agatgaagaa gccgagcgtg acgaagccca tgtgcgagat cgacgaatag
    10441 gcgatcagct tcttcatgtc ggcctgcacc agcgcgacga gcccgatgta gaccaccgcg
    10501 atgagcgaga gcgcgatcat gaaccaggcc agctcgcgcg aggcgtcggg caggatcggc
    10561 agcgtgaagc gcacgaagcc gta
//
LOCUS       DATDAA010000025         5652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_2081,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000025 DATDAA010000000
VERSION     DATDAA010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2081"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VIS73_00905"
     CDS             complement(<1..286)
                     /locus_tag="VIS73_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HEY5761744.1"
                     /translation="MRRRASGGVAAAALLLSLAVLSGCATIPEGVGTSPQDPWERYNR
                     HMDAFNDGLDRAVIKPAAQAYDAVTPLPVRVVVNNFLGNIGDLWVGVNSLM"
     gene            complement(286..753)
                     /gene="mlaD"
                     /locus_tag="VIS73_00910"
     CDS             complement(286..753)
                     /gene="mlaD"
                     /locus_tag="VIS73_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEY5761745.1"
                     /translation="MNRKAIDLWVGIFVAIGFASLLFLSLRVGNLTSSDLGTTYRLDA
                     RFDNVGGLKVRGPVRSAGVVVGRVTAVRFDAETFQAVVSLDIDERYRFPRDTFASILT
                     SGLLGEQYVGLDAGGDEAMFKAGDTIIKTQSAMVLEKLVGQFLFDKAADGEKK"
     gene            complement(753..1535)
                     /gene="mlaE"
                     /locus_tag="VIS73_00915"
     CDS             complement(753..1535)
                     /gene="mlaE"
                     /locus_tag="VIS73_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEY5761746.1"
                     /translation="MTALLERLGHGVTRRLERLGYASRFFVALLANSPTSFLRFRLTM
                     REIWFAGVLSLIIIIVSATFVGMVLGLQGYNTLQRYGSSEALGVLVALSLVRELGPVV
                     AALLFASRAGSAITAEIGLMKTTEQLSAMEMMAVEPVARVVAPKFWGGVISMPFLAAL
                     FSTMGVFGGWLVGVVLIGVDDGAFWSQMQAAVDFREDILNGVIKSVVFGVAVAFIAVF
                     EGYDAPPTAEGVSGATTRTVVTSALAILALDFILTAFMFRGL"
     gene            complement(1532..2353)
                     /locus_tag="VIS73_00920"
     CDS             complement(1532..2353)
                     /locus_tag="VIS73_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5761747.1"
                     /translation="MPNQSPSDALVEIDQLDFAYDERKVLDGVSLRVPRRQLVAIMGG
                     SGCGKTTLLRLIGGQLRPSRGSVRIDGETVHKAGYEGLYRLRRRMGMLFQFGALFTDL
                     SVFDNVAFPMREHTDLPESVIRDLVLLKLNAVGLRGAAQQMPASLSGGMARRVALARA
                     IALDPQLMLYDEPFTGLDPISMTIVGQLIRRLNDALGASSVIVTHDVHESLALVDYLY
                     IMSAGKVVAEGTPEEIKASTDPFVHQFVWAEPDGPVPFHVAAPALADAFISGAQA"
     gene            2498..3925
                     /locus_tag="VIS73_00925"
     CDS             2498..3925
                     /locus_tag="VIS73_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5761748.1"
                     /translation="MNTPQRPLLLIVDDDPLITDALGYALGRDFEILTSATRPQAMRL
                     LHQLRAPPAAALVDLGLPPIPHQPDEGFALISELAAQAPDTRIVVLSGQDDEAHARHA
                     RTLGAADFIGKPCNPSELADRLHRLLAVGGRPAAARDDAWALVGTSPAINDLRDKATQ
                     FADSPFPVLIEGESGSGKEVVARGCLHARSRRADRPFIALNCAAIAPTLVEPTLFGHA
                     KGAYTGAQQARSGYFEDAADGTLFLDEIGELPVDAQAKLLRVLETGEYQRVGETQTRH
                     VRARIIAATNRDLRLEVRENRFRSDLYHRLSVLTITVPPLRELGDDRLLLLDHFLARY
                     VQESSQPPFILDTEARRLWRAYDFPGNVRELRNIVIRLLAKYPAQTIDSTLLAGEFEI
                     APSLGGATTALPEDMNTLLPLAIARLRQDEDFDLDHALRLYERALIDAALEVASGNVS
                     KAARLLNISRTTLYSRMEAREKARA"
     gene            3922..5403
                     /locus_tag="VIS73_00930"
     CDS             3922..5403
                     /locus_tag="VIS73_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5761749.1"
                     /translation="MSMYLNHFGLREVPFSITPHTEFFFSGANRGATLEALVYAVTHD
                     EGIVKVTGEVGSGKTMLCRMLLERLPANVATVYLANPSLSRDEILYALSDELAMPAQP
                     QRVHQLLRGLQAHLVDLYAAGRQVVVLIDEAHAMPPESLEEVRLLSNLESKRHKLLKI
                     VLFGQPELDEMLSRSEMRQLRERVTHNFGLEPLTQTDIGGYLMFRMRAAGYHGPDLFT
                     DAAVRLIAEASLGLSRRINIVADKALLAAFVDGEHQIGVKQVKVAIKDAQFGALPGRS
                     RRSNSRPLLLAGAMAALATVVAVSFWSLRASGSAVVQAPPAPSAVAAELERVTPAAAE
                     SPSDNKPATTDQMPASPLGLQNPVATSEAPLPLQASAELSAPIAAHEAGSDPAPISLT
                     TAFSDAYSRWINDAAGKNYFIQLMRTNTTDHQDVERYLANTARLVDPESLRIYQSQAD
                     GQTWLGVVYGDFPSLEAALAAIDALPAPLREAGAYPRRVAMLK"
BASE COUNT         1112 a         1934 c         1684 g          922 t
ORIGIN      
        1 gcatcaggct gttcacgccg acccaaaggt caccgatgtt gccgagaaaa ttgttgacca
       61 cgacacgcac cggcagcggc gtcaccgcgt cgtaggcctg cgccgccggt ttgatcactg
      121 cacggtcgag tccatcgttg aaggcgtcca tgtgacggtt gtagcgttcc cacgggtcct
      181 gcgggctggt cccgacacct tcgggaatcg tcgcgcaacc cgaaagcacc gccagcgaca
      241 gcagcagcgc cgcagcagca acccctcccg acgctcggcg gcgcactact tcttctcgcc
      301 gtcggcagcc ttgtcgaaca ggaactgtcc gaccagcttc tcgagcacca tcgccgactg
      361 cgtctttatg atcgtgtcgc cggccttaaa catcgcctcg tcgccaccgg cgtcgaggcc
      421 gacgtactgc tctccgagca ggccggaggt caagatcgag gcgaaggtat cgcgcggaaa
      481 ccggtaacgc tcatcgatgt cgaggctgac cacggcctgg aaggtctccg catcgaagcg
      541 aactgccgtg acgcggccga ccaccacgcc ggcgctgcga accggcccgc gcaccttgag
      601 tccgccaacg ttgtcgaagc gtgcatccag ccggtaggtg gtgcccagat cgctcgaggt
      661 gagattgccg acccgcagcg acaggaacaa gagagaggcg aagccgatcg caacaaaaat
      721 gccgacccaa aggtcaatgg ctttgcggtt cattacaacc cccggaacat gaaagcagtc
      781 aggatgaaat cgagcgcgag gatggccagc gccgaggtga cgaccgttct cgtggtcgca
      841 cccgagacgc cctcggccgt gggtggcgca tcgtagcctt cgaagacagc gatgaacgcg
      901 accgcaacgc cgaagacgac actcttgatc acgccgttga gaatgtcctc gcgaaaatcc
      961 acggccgcct gcatctgcga ccagaaggca ccatcgtcga ccccgatcaa tacaacgccg
     1021 accagccagc cgccgaaaac gcccatcgtc gaaaacagcg cggcgagaaa aggcatcgag
     1081 atgacgccgc cccagaactt cggtgcaacg acacgggcga ccggttcgac cgccatcatt
     1141 tccatcgccg acaactgctc ggtggtcttc atcaaaccga tttcggccgt gatcgccgag
     1201 ccggcgcggc tggcgaagag cagggcagcc acgaccggcc ccagctcacg gactagcgac
     1261 agtgcaacca gaaccccaag cgcttccgac gaaccgtaac gctgcaaggt gttgtagccc
     1321 tgcagcccga gcaccatgcc gacgaaggtc gccgaaacga tgatgatgat cagtgacaga
     1381 acgccggcaa accagatctc gcgcatggtc agccggaagc gcaggaagga tgtcggcgaa
     1441 ttggcgagca gcgcgacaaa gaagcgcgag gcgtagccca gtcgctcaag acgccgcgtc
     1501 acgccatgcc ccaggcgttc gagcagcgcg gtcatgcctg cgctccactg atgaatgcgt
     1561 cggcaagcgc cggtgcggct acgtggaaag gcactggccc gtcgggttcg gcccagacga
     1621 actgatggac gaagggatcg gtcgaggcct tgatttcctc gggcgttcct tcggcaacga
     1681 ccttgcccgc cgacatgata tataggtaat cgaccagcgc cagcgattcg tggacatcgt
     1741 gagtgacgat caccgaactg gccccgagcg catcgttcag ccgccggatc agttgaccga
     1801 cgatggtcat cgagatcggg tcgaggccgg tgaatggctc gtcatagagc atcagctggg
     1861 ggtccagcgc aatcgccctt gccagcgcca cacgccgcgc catgccgccc gagagcgacg
     1921 caggcatctg ctgcgcagcc ccgcgcaaac cgacggcgtt aagcttgagc agcaccagat
     1981 cgcggatcac gctttccggc agatcggtgt gctcacgcat cgggaaggcc acgttgtcga
     2041 agaccgacaa atcggtaaac aaggcgccga actggaacag cattcccatt cgccgccgca
     2101 ggcggtaaag gccttcgtag ccggccttgt ggacggtctc gccgtcgatg cggacgctgc
     2161 cgcggctcgg tcgcaactgc ccaccaatca gacgcagcag cgtggtcttg ccgcagccgc
     2221 tgccgcccat gatcgccacc agctgccgtc gcgggacgcg caggctgacc ccgtcgagca
     2281 ccttgcgctc gtcataggcg aagtcgagtt ggtcgatttc gaccagtgcg tcggaagggg
     2341 attggttcgg catgcgcacg ccgcgcagaa gggcgaagtc cgggaccgca aatggtagca
     2401 gatgcgactt agtgcccaat gaccgcgcca agcaatcacg gacaaggcta aaaagttgct
     2461 ctagaatcag ggacgttcag ccacttcttg agaagtcatg aacactccgc agcgccccct
     2521 gctgctcatc gtcgacgatg atccgctgat caccgacgcg ctgggctatg cactcggccg
     2581 agacttcgaa atcctgacca gcgccacccg cccacaggcg atgcgcttgc tgcaccaatt
     2641 gcgcgcgcca ccggccgctg cgctggtcga cctcggcctg ccgccgatcc cgcaccagcc
     2701 ggacgaaggc ttcgccttga tttcagagct ggcagcgcag gctcccgata cgcgcatcgt
     2761 cgtgctctcg gggcaagacg acgaagccca tgcccgccac gcccgcacgc tgggcgccgc
     2821 cgatttcatc ggcaagccct gcaatccgtc cgaactggcc gaccggctgc accgcctgct
     2881 tgccgttggc ggtcgcccgg cagctgcccg tgacgacgcc tgggcgctgg tcggcacaag
     2941 cccggccatc aacgaccttc gcgacaaagc aacgcaattt gccgattcac ccttcccggt
     3001 actgatcgag ggcgagtccg gcagcggcaa ggaagtcgtc gcccgcggct gcctgcacgc
     3061 cagaagccgc cgcgcagacc gaccgttcat tgccctcaac tgcgcggcaa tcgcgccgac
     3121 gctggtcgag cctacgcttt tcggtcacgc caagggcgca tacaccggcg cgcagcaggc
     3181 ccgctcgggg tatttcgagg acgccgcgga cggcaccttg ttcctcgacg agatcggcga
     3241 actgccggtc gacgcccagg caaaactgct gcgcgtactc gagaccggtg aataccagcg
     3301 cgtcggcgag acccagacgc ggcatgtgcg cgcgagaatc atcgccgcga ctaaccgcga
     3361 cctgcgcctc gaggttcgcg agaaccgctt ccgcagcgac ctctaccatc gcctcagcgt
     3421 gctgacgatt accgtgccgc cactacgcga gctcggcgat gaccggctgc tgttgctcga
     3481 ccatttcctt gcccgctacg tccaggagtc gtctcaaccg cccttcatcc ttgacaccga
     3541 ggcccggcgc ctgtggcgcg catatgactt cccgggcaac gtccgcgaac tgcggaatat
     3601 cgtcatccgg ctgctggcca agtacccggc gcagaccatc gattccaccc tgcttgccgg
     3661 cgagttcgag atcgccccca gtcttggcgg tgcgacgacg gccctgcccg aggatatgaa
     3721 caccctgttg ccgctggcca tcgcccggct gcggcaggac gaagacttcg acctcgacca
     3781 tgcccttcgc ctgtacgaac gggccctgat cgatgccgcg ctggaggttg ccagcggcaa
     3841 cgtcagcaag gccgcccgcc tcctcaatat cagccgcacc accttgtata gccgcatgga
     3901 agcacgcgag aaagcccgcg catgagtatg tatctcaatc acttcggcct ccgtgaagtg
     3961 ccattcagca tcacgccaca tacggagttc ttcttctccg gcgccaatcg cggcgccacg
     4021 cttgaagccc tcgtctacgc cgtcactcac gacgagggca tcgtcaaggt caccggcgaa
     4081 gtcggcagcg gcaagaccat gctctgccga atgctgctcg agcgtctgcc cgccaatgtc
     4141 gcgaccgtct atctcgccaa tccttcgctg tcgcgcgacg aaatcctcta cgcgctgtct
     4201 gatgaactgg ccatgccagc acagccgcaa cgcgtccacc agctgctgcg cggcctgcag
     4261 gcgcatctgg tagatctcta cgcggcaggc cgacaggtgg tcgtcctgat cgacgaagcg
     4321 cacgcgatgc cacccgagtc gctcgaagaa gtgcgcctgc tgtccaatct cgaatcgaag
     4381 cggcacaagt tgctgaagat cgttctcttc ggccagcccg agctcgacga gatgctctcg
     4441 cgcagcgaga tgcgccagtt gcgcgagcgc gtgacccaca acttcggcct cgagccactg
     4501 acgcagacgg acattggcgg ctacctgatg ttccgcatgc gcgccgccgg ttaccacggc
     4561 cccgacctat tcaccgacgc cgccgtgcgc ctgatcgctg aggcctcatt gggcttaagc
     4621 cggcgcatca atatcgtcgc cgacaaggcg ctgctcgctg ccttcgtcga cggcgaacac
     4681 cagatcggcg tcaagcaggt caaggtggca ataaaggacg cccagtttgg cgccctgccg
     4741 ggccgttcgc ggcggagcaa ttcccggccg cttttgctcg ccggggcgat ggccgctctg
     4801 gcaaccgtcg tcgcggtctc cttctggtcg ctgcgcgcta gcggcagcgc ggtggtgcag
     4861 gcaccaccag ccccgtcagc ggtagcagcc gagctagaac gcgtcacgcc agccgccgcg
     4921 gaaagcccgt cggataacaa gcctgctaca accgatcaga tgccggcatc gccgttgggc
     4981 ctgcagaatc ccgtggccac cagcgaagcg ccgctaccgc tgcaggcatc ggccgaactg
     5041 tcggcgccga ttgccgccca cgaagcaggc tcagatcccg cgccgatttc gctgacaacc
     5101 gccttcagtg acgcttattc gcggtggatc aacgacgcgg cggggaagaa ttacttcatc
     5161 cagctcatgc gaaccaatac gaccgaccac caggatgtcg agcgctacct ggcgaacacg
     5221 gcccgactgg tcgatcccga gtccctccgc atctaccagt cgcaggccga tgggcagacc
     5281 tggcttggcg tcgtctatgg tgactttccg agtcttgaag ccgcgctcgc ggcgattgac
     5341 gccctcccag cgccgctgcg cgaggctggc gcctatcctc gccgcgtagc aatgctgaaa
     5401 tgagtactag tacctgcgcc cgggacttcc gtgtacctgc ccgaagcccg gtcgttctat
     5461 atagtcagaa tcgccctctg tgtgaggatt cagcggcgcg cagccgcatg ttaatgtgct
     5521 aatgttttca agagcccgtc cgccagggaa gaccatatga agaagccgat gaaatccggt
     5581 gctgcgatcg tttcagtgtt cctcgcggcg tgcgcaacac cgaccgcaaa accacctgcg
     5641 gaaatgcacc tg
//
LOCUS       DATDAA010000026         2722 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_107054,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000026 DATDAA010000000
VERSION     DATDAA010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107054"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..988
                     /locus_tag="VIS73_00935"
     CDS             128..988
                     /locus_tag="VIS73_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY5761750.1"
                     /translation="MAPRGNRAPPREGAQPRSRGKRGEARHGESVMDSEPVRLHKILA
                     QVGVGSRRELEDWILAGRISVNGKPAEVGQLVGPSDRIKVNGRLINVNFSQRMPRVLV
                     YNKPEGEIVSRDDPEGRPSVFDALPRINGGRWIAVGRLDFNTSGLLVFTTSGELANRL
                     MHPRYEIIREYAVRILGDLDEEAKHELLDGVMLEDGMARFHLLEEAGGEGANRWYRVI
                     LHEGRNREVRRMFEAVGATISRLMRVRYGPLNLPPNLRRGKVTELDERNIRALLDAVG
                     MAAPKPKFVR"
     gene            1108..1518
                     /gene="rpsF"
                     /locus_tag="VIS73_00940"
     CDS             1108..1518
                     /gene="rpsF"
                     /locus_tag="VIS73_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY5761751.1"
                     /translation="MRHYEICFIVHPDQSEQVPAMVERYRTLVTAGNGSIHRLEDWGR
                     RQMAYPIQKMHKAHYVLMNIECDNAVLAELEHAFKFNDAVLRHLVVKMKHAVTAPSPM
                     MKEEKAKSLIGGEAKPAEEAAAPAAAAATPAAAS"
     gene            1445..1831
                     /gene="priB"
                     /locus_tag="VIS73_00945"
     CDS             1445..1831
                     /gene="priB"
                     /locus_tag="VIS73_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEY5761752.1"
                     /translation="MAKRSRPKKLLRQLLLPRRRLPRPDQPRRANKLNQYCVSGALIE
                     RAATRYTPAGIPVCEAKLAHDEAATEAGGERQVTFELAVLGLGEAAKWLEAAALGTRV
                     RLEGFLAPKSQNSRQLVLHLNRIEFL"
     gene            1841..2122
                     /gene="rpsR"
                     /locus_tag="VIS73_00950"
     CDS             1841..2122
                     /gene="rpsR"
                     /locus_tag="VIS73_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY5761753.1"
                     /translation="MSRPYGKKKTDGKKRGPRGGGLFKRRKYCRFTAEKVELIDYKDV
                     DVLKEFITETGKIIPARLTGTKAGYQRQLADAIKRARFMALLHYTDNHQ"
     gene            2133..2582
                     /gene="rplI"
                     /locus_tag="VIS73_00955"
     CDS             2133..2582
                     /gene="rplI"
                     /locus_tag="VIS73_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY5761754.1"
                     /translation="MQVILMEKVVNLGNLGDIVRVKDGYARNFLIPRGKARRATESAK
                     AEFEARRADLETVANEKLAQAQAFAEKINGLMVQVTRKAGVDGRLFGSVSNADIAEAL
                     GAQGHEVHKQAVRLPSGPIKAVGDYPVELDLHTDVVAHVTVSVLGEH"
BASE COUNT          555 a          900 c          849 g          418 t
ORIGIN      
        1 gcagggcgcc cggccgcacc gccagggcgg tggggacggg gagtcgcgcg gcaatcgcgc
       61 gccgcggcag ggtggcgacg acggcacctc ctacggcaac cggcagcatt tcgacggcgg
      121 cgacgagatc gcgccgcgcg gcaaccgagc gccgccgcgc gaaggcgccc agccgcgcag
      181 ccgcggcaag cgtggcgagg cccgccacgg cgaatcggtg atggacagcg agccggtacg
      241 cctgcacaag atcctcgcgc aggtcggcgt cggttcgcgg cgcgaactcg aggactggat
      301 tctcgccgga cgcatcagcg tcaacggcaa gcctgccgaa gtcgggcagt tggtcggccc
      361 gagcgaccgc atcaaggtca acggccggct gatcaacgtg aacttctcgc agcgcatgcc
      421 gcgcgtgctg gtctacaaca agcctgaagg cgagatcgtc tcgcgcgacg atcccgaagg
      481 ccgtccctcg gtcttcgacg cgctgccgcg aatcaacggc gggcgctgga tcgccgttgg
      541 ccggctcgac ttcaatacca gcggcctgct cgtctttacc acctcgggcg aactcgccaa
      601 ccgactcatg cacccgcgct acgagatcat ccgcgagtac gccgtgcgca tcctcggcga
      661 cctcgacgaa gaggccaagc acgaactgct cgacggagtc atgctcgaag acggcatggc
      721 gcgcttccac ctgctcgaag aagccggcgg cgagggtgcc aaccgctggt accgcgtgat
      781 cctgcacgaa ggccgcaacc gcgaggtgcg gcgcatgttc gaagcggtcg gcgccaccat
      841 cagccggctg atgcgcgtgc gttacggccc gctcaacttg ccgcccaacc tgcggcgcgg
      901 caaggtcacc gagctcgacg agcgcaacat ccgcgccctg ctcgacgccg tcggcatggc
      961 cgcgcccaag cccaagttcg tgcgctgagc ccgaactacc tgccgaaact ggcgctggcg
     1021 gcgaatttcg gctagaatcg cggccctttt gcagccttgc tccaccgcaa acgcatggcg
     1081 gggggcttta acccacaagg agaattcatg cgccattacg aaatttgttt catcgtccat
     1141 ccggaccaga gcgagcaggt cccggcgatg gtcgagcgtt accgtacgct cgtcaccgct
     1201 ggtaacggca gcatccatcg tctcgaagat tggggtcgtc gtcagatggc ctacccgatc
     1261 cagaagatgc acaaggcgca ctatgttctg atgaacatcg agtgcgacaa cgccgttctt
     1321 gccgaactcg agcacgcctt caagttcaac gatgccgttc tccgtcacct cgtcgtcaag
     1381 atgaagcacg ccgtcacggc cccttcaccg atgatgaagg aagagaaagc caagtcgctg
     1441 atcggtggcg aagcgaagcc ggccgaagaa gctgctgcgc cagctgctgc tgccgcgacg
     1501 ccggctgccg cgtcctgatc aaccgagaag agcgaacaag ctgaaccaat actgcgtttc
     1561 gggcgcactg atcgagcgag cggcaacccg ctatacaccg gccggcattc cggtctgcga
     1621 agcgaagctg gcgcacgatg aggcagcaac cgaagccgga ggcgagcgac aggtcacttt
     1681 cgaacttgcg gtactcggct taggcgaggc agccaaatgg ctggaagccg cagcactcgg
     1741 tacccgggtc agactcgaag gcttcctcgc cccgaaatcg cagaattcac gacagcttgt
     1801 attgcacctg aaccgaatcg aatttctgta aggaaacaac atgtcacgac cgtatggaaa
     1861 aaagaagact gacggcaaga agcgcggccc ccgtggtggc ggcctgttca agcgccgcaa
     1921 gtactgccgc ttcaccgccg agaaggttga gctgatcgac tacaaggatg tcgacgtgct
     1981 caaggaattt atcactgaga ccggcaagat catcccggcg cgcttgaccg gcaccaaggc
     2041 cggctaccag cgccaactgg ccgacgcgat caagcgcgcc cgcttcatgg cgctgctgca
     2101 ctacaccgat aaccaccagt aaggagacca gcatgcaagt gatcctgatg gaaaaggtgg
     2161 tcaacctcgg caaccttggc gacatcgttc gcgtcaagga cggctacgcc cgcaacttcc
     2221 tcatcccgcg cggcaaggcc cgtcgtgcca ccgaatcggc caaggccgaa ttcgaagcgc
     2281 gtcgtgccga cctcgaaacc gtcgccaatg aaaagctcgc gcaggcgcag gccttcgccg
     2341 agaagatcaa cggcctgatg gtccaggtca cccgcaaggc cggcgtcgat ggccgcctct
     2401 tcggctcggt cagcaacgcc gacatcgccg aggcgctcgg tgcgcagggc cacgaagtcc
     2461 acaagcaggc cgtgcgcctg cccagcggcc cgatcaaggc cgtcggcgac tacccggtcg
     2521 aactggacct gcacaccgac gtcgttgccc acgtcaccgt ctctgtgctc ggcgagcact
     2581 gatccggtcg gcttcggccg aatcgaaaag ccccgcgtga gcggggcttt ttctttgggt
     2641 agcgggaatt cactgtggat gccagtgacg tagaaaccgc ttgacggttg gctgccttgg
     2701 ccggcagagt tcggccagga gc
//
LOCUS       DATDAA010000027         5424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_281630,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000027 DATDAA010000000
VERSION     DATDAA010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281630"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VIS73_00960"
     CDS             complement(<1..1020)
                     /locus_tag="VIS73_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY5761755.1"
                     /translation="MSQSEFAYSYDQLRDRVSDALVYARRQGADAVEVEASEGFGMSV
                     TVRQQALETIEYNRDKGVSVTVYLGQRKGYASTADFSAKALRDTVNAALDIARFTAPD
                     PCAGLPEASLLASGSADLDLHRPWSIDVEQAAEIARRCESAALGVSDQISNSEGASLS
                     TQAGQFCMGNSLGFLGGYPGSRHYIACSVIAGKGNAMQRDDWYTTGRDPKALAAPEEV
                     GEYAARRALSRLGARKVKTCEVPVIFEAPLACALLGNFVHAASGGSLYRKASFLRDRL
                     GQRVFSAPVSIDERPHLRGAMASAPFDNDGVATDDRTVVADGVLEGYFLSTYSARKLG
                     MTTTAN"
     gene            1107..1637
                     /gene="yjgA"
                     /locus_tag="VIS73_00965"
     CDS             1107..1637
                     /gene="yjgA"
                     /locus_tag="VIS73_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEY5761756.1"
                     /translation="MSRPKTPRQAVPEDQPEAPSKTQRKAAMQALQDLGEQLVALPRD
                     RIAKLPLDETLRSAVAEIGRLSAHEARRRQMQYIGRLMRDADTEAIAEAVAAFAGASR
                     ADTARLHRLEDLRDRLLADDAVLAEIGSEHPGADLQRLGQLRRNAIREREQGAAPRSY
                     RALFQLLKEIAEGKPN"
     gene            1634..2197
                     /gene="mog"
                     /locus_tag="VIS73_00970"
     CDS             1634..2197
                     /gene="mog"
                     /locus_tag="VIS73_00970"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEY5761757.1"
                     /translation="MSDSSEKPLHLGLVSISDRASTGVYEDRGLPELERWFGETLTSP
                     WHASKRLIADEQATIESTLIELADREGCQLILTTGGTGPALRDVTPEATLAVADKVLP
                     GFGEEMRRVSLAYVPTAILSRQTAVVRGSALIINLPGQPKAIRETLDGIFAAVPYCID
                     LIGGPYVTTDEARIKAFRPKSAIRPSP"
     gene            complement(2228..3739)
                     /locus_tag="VIS73_00975"
     CDS             complement(2228..3739)
                     /locus_tag="VIS73_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761758.1"
                     /translation="MIIGLFNRESGHPLEDAREVRRVIAELPTEQSVKAVDEIAGWFE
                     SLASAVDFRGDRLYDVVNLLDQAAQAHVRKLTRDYLAAVRAGHSDERAIWAHGRFYWE
                     QLAGLYERIIEAAGQKDKVGEYLKPNLPVISCRLLSAYVAQIKWGHFRHEQPSRSLWE
                     RIGHVYLDADQAKYATKVVALYSALSGNASVEQEFLRAAILGSSSLEALQPVETELVD
                     RLVTHLLAEFVIARGNVPGMLYWIDAATGEPPSRLARLPQPSPSVRLIAPGNAPAVVA
                     ELSHAAERGSLPPELNLGAEYPARLVLRALRHLATYLSPAPPMRQHKRHPVKTRLSVV
                     SGLIATMEALGGVLPAEADDWWSENVSLGGFGAVIEKAALDSKVGVGTLVCVRADGGN
                     SWVVGLVRRYARVDGGATQVGIQALTRQARIGEFVPRASGTYMAAGSFSGVVIADQEI
                     GDEIRVLLPPTRFDLRENLDLRLGEQRFLLLPAECIESSVDFDFARYRVRPVA"
     gene            complement(3765..5237)
                     /locus_tag="VIS73_00980"
     CDS             complement(3765..5237)
                     /locus_tag="VIS73_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEY5761759.1"
                     /translation="MLANLEFPGKAPRGKASRVMHRLCIIDGDNAVGISAKASVAAVP
                     DEHELLRNVLRRRRKTMADLSTTPIAATDDKGGLIVWIRWDSRASRFERFTALRKAVA
                     ILLGDGAERLSVSLSSGLPESAANETLYVCWINGVTLPSARAEPERKPLAAIVWNGKQ
                     PDHQAVRAIADGNVLARRLTAMAPNVLTPKALRLEAEAIARRSGYTVLTHGIKALQKL
                     GCGAFLSVAQGSDDDEAAIVRLRYQPRRARGRVALVGKGICFDTGGYNVKPARAMAGM
                     HEDMAGAAVVLGLMQAVSALRLPLAIDGWLAISRNRIAPGASVPGDVVRACNGKTIEI
                     VHTDAEGRMVLADTLALAARDKPDLVLDFATLTGSMVVALGCRMAGVLSPDAQWAARA
                     ADAGARAGERLVAFPVPEDYAADIESKVADLKQCTPDGEADHILAASFLRNFVGKSPW
                     LHVDLSAVHCKGGLGAVDSELTGFGVAAGVEFLRDLSCSL"
     gene            5305..>5424
                     /locus_tag="VIS73_00985"
     CDS             5305..>5424
                     /locus_tag="VIS73_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY5761760.1"
                     /translation="MHPYLNLMRHVLENGHRKSDRTGTGTLSVFGWQMRYALDE"
BASE COUNT          944 a         1873 c         1750 g          857 t
ORIGIN      
        1 gttggcggtg gtggtcatgc ccagcttgcg cgccgaatac gtgcttagga agtagccctc
       61 cagtacgcca tcggcgacca ccgtgcgatc gtcggtcgcg accccatcgt tgtcgaaggg
      121 tgccgaggcc atcgcgccgc gcagatgcgg acgctcgtcg atcgagacgg gtgcggaaaa
      181 gacgcgctgt cccagacgat cacgcaagaa gctggctttg cggtagaggc tgccaccgct
      241 cgccgcatgg acaaagttgc cgagcagcgc gcaggccagc ggcgcctcga agatcaccgg
      301 cacctcgcag gtcttgacct ttctcgcgcc gagccgcgac agggcacgcc gcgcggcgta
      361 ttcgccaact tcctcgggtg cagcgagcgc cttcgggtcg cggccggtcg tataccagtc
      421 gtcgcgctgc atcgcgttgc ccttgccggc gatcaccgag caggcgatgt aatgtcgcga
      481 gcccgggtaa ccgccgagga aaccaagcga gttgcccatg cagaactggc cagcctgcgt
      541 cgacaggctc gcaccttcgg agttgctgat ctgatcgctc acgcccagcg ccgccgattc
      601 gcagcgccgg gcgatctcgg cagcctgctc gacgtcgatg gaccacgggc ggtgcaggtc
      661 gaggtcggca ctgccgctcg ccagtagact ggcctcgggc agcccggcgc aggggtcggg
      721 cgcggtgaaa cgggcgatgt cgagcgcggc gttgacagtg tcgcgcaacg ccttcgccga
      781 gaaatcggcg gtactcgcgt aacccttgcg ctggccgagg tagaccgtga cgctgacgcc
      841 cttgtcgcgg ttgtactcga tggtttccag cgcctgctgg cgaaccgtga cagacatccc
      901 gaagccttct gaggcctcga cctcgaccgc gtcggcgcct tgtcgccgcg cgtagacgag
      961 tgcatcggaa acccgatcac gcagctgatc gtaggaatag gcgaactcgc tctggctcat
     1021 cgttgggacc tgtcgacgcg gcgcgtgggc gccgcgtggc ttgcgggggc gctatgatag
     1081 caagcgatca acaagctacg tttcccatgt cccgcccgaa aaccccgcgc caagccgtcc
     1141 ctgaagacca accagaagcc ccgagcaaga cccaacgcaa ggcggccatg caggccctgc
     1201 aggatctcgg cgaacaactc gtcgcgctgc cccgcgaccg cattgccaaa ttgccgctcg
     1261 acgaaaccct gcgcagcgcc gtcgcagaga ttgggcgact gtcagcgcac gaggcgcggc
     1321 gccgacagat gcagtacatc ggtcggctga tgcgcgacgc cgacaccgag gccatcgccg
     1381 aggccgtcgc cgccttcgcc ggcgcttcac gcgccgacac ggcgcgcctg catcgcctcg
     1441 aagacctccg cgatcgcttg ctggccgacg atgccgtgct tgccgaaatc ggcagcgagc
     1501 accctggcgc cgatcttcag cgccttggcc agttgcgtcg caacgccatt cgcgagcgcg
     1561 aacagggggc ggcgccgcgc agctaccgcg cgctgttcca actcctcaag gaaattgccg
     1621 aagggaaacc gaattgagcg attcaagcga aaaaccgttg catttgggcc tggtgtcgat
     1681 ctctgaccgc gcgtcgacag gggtctacga agatcgtgga ctgccggagc ttgagcgctg
     1741 gtttggcgaa acactgacct cgccctggca cgccagcaag cgcctgattg ccgatgagca
     1801 ggcgacaatc gaatcgacgc tgatcgaact ggccgaccgc gaaggctgcc agctgatcct
     1861 caccaccggc ggcaccggac cggcgttgcg cgacgtcacg cccgaggcga ctctggccgt
     1921 ggccgacaag gtgctgccgg gtttcggaga ggagatgcgc cgcgtcagtc tcgcctacgt
     1981 gccgaccgcg atcctctcgc ggcaaaccgc cgtggtgcgc ggcagcgcgc tgatcatcaa
     2041 cctgccgggt caacccaagg cgattcgcga aacgctggac ggcatcttcg ccgcggtgcc
     2101 ctactgcatc gacctgatcg gcggccccta tgtcaccacc gacgaagcgc ggatcaaggc
     2161 gttccgaccc aagtcagcga tccggccctc gccctgaggc tgcggctaac gccgcgcaag
     2221 ccgccgctca ggcgaccggc ctgacgcgat agcgcgcgaa atcgaaatca acgctcgact
     2281 cgatgcactc ggccggcagc agcaggaaac gctgttcgcc gaggcgcaag tcgaggttct
     2341 cgcgcagatc gaagcgggtc ggtggcagca gcacgcggat ttcgtccccg atttcttggt
     2401 cggcgatgac gacgccggaa aacgatccgg ccgccatgta ggttcccgac gcccgcggca
     2461 cgaactcgcc gatgcgcgcc tgtcgcgtca gcgcctgaat accgacctgc gtcgcgccgc
     2521 cgtcgactcg cgcgtatcgc ctgaccagac caaccaccca gctgttgccg ccatcggcac
     2581 gcacgcagac taaggtaccg acgccgacct tgctgtcgag cgcggccttc tcgatgaccg
     2641 ccccgaaacc accgaggctg acgttctccg accaccagtc gtcagcctcc gcgggcagca
     2701 cgccacccag cgcctccatg gttgcgatca gaccgctgac gacagacagc cgcgtcttca
     2761 ccgggtgccg cttgtgctgg cgcatcggtg gcgccggtga aagataggtg gcgaggtggc
     2821 gcaaggcccg gagcaccagc cgcgccgggt actcggcacc gagattcagc tcgggcggga
     2881 ggctgccgcg ctcggccgca tggctcagtt cggcaacgac tgccggcgcg ttgcccggcg
     2941 cgatcaggcg caccgacggc gatggctgtg gcaagcgtgc cagacggctc ggcggctcgc
     3001 cggtggcggc atcgatccag tagagcatcc ccggcacatt gccgcgggcg atcacgaact
     3061 ccgcaagcag atgtgtcact agccgatcga ccagttcggt ctcgacgggc tgcagtgcct
     3121 cgagcgacga actgcctaat atcgccgccc gcagaaactc ctgttcgaca ctcgcgttac
     3181 ccgacagcgc ggagtagagc gccaccacct tggtcgcgta cttggcttgg tcagcgtcga
     3241 gatagacatg cccgatccgt tcccagagcg aacgggacgg ctgctcgtgg cggaaatgac
     3301 cccacttgat ctgcgctacg taggccgaga gaagccggca gctgatgacc ggcaggttcg
     3361 gcttgagata ctcgccgacc ttgtccttct gccccgccgc ctcgatgatc cgctcgtaga
     3421 ggccagcaag ctgctcccag tagaatcgcc catgcgccca gatcgcgcgt tcgtcgctgt
     3481 gccccgcgcg caccgccgcc agatagtcgc gcgtcagctt gcgcacgtgc gcctgcgctg
     3541 cctgatcgag caggttcacc acatcgtaga ggcgatcgcc acggaaatcg acggccgagg
     3601 ccagcgattc gaaccagccg gcgatctcat cgacggcctt caccgactgc tcggtcggca
     3661 attccgcaat gacgcgccgg acctcgcggg catcttcgag cggatggccg gattcgcggt
     3721 tgaacaggcc gatgatcaat gcttcccctt atgcggacga cgcctcacag gctgcaggag
     3781 aggtcgcgca gaaattcgac gccggcggcg acgccgaaac cggtcaattc gctgtcgacc
     3841 gccccgagcc cgcccttgca gtgcactgcc gacagatcaa catgcagcca gggggacttg
     3901 ccgacgaagt ttcgcaagaa agacgcggcg aggatatggt cggcctcgcc atccggcgtg
     3961 cattgcttga ggtcggcaac cttgctctcg atgtccgccg cgtagtcctc gggcaccggg
     4021 aaggcgacca atcgctcgcc ggcacgcgcg ccggcgtcgg ccgcccgcgc cgcccactgg
     4081 gcatcgggcg aaagcacccc ggccatgcgg cagccgagcg cgacgaccat gctgccggtc
     4141 agcgtcgcga agtcgaggac gaggtcgggt ttgtcgcgtg ctgccagtgc cagcgtgtcg
     4201 gcgagcacca ttcggccctc ggcatcggtg tgtacgatct cgatcgtctt gccgttgcag
     4261 gcacgaacaa cgtcaccggg aacactggcg ccgggcgcga tgcggttgcg cgaaatggcg
     4321 agccagccgt cgatcgccag cggtaagcgg agggcgctga cggcctgcat caggccgagg
     4381 accaccgcgg cgccggccat gtcttcgtgc atgccagcca tcgcccgcgc cggcttgacg
     4441 ttgtaaccgc cggtgtcgaa acagattccc ttgccgacca gcgccacccg cccgcgcgcg
     4501 cggcgcggtt gatagcgcag gcggacgatc gccgcctcgt cgtcgtcgct gccctgcgca
     4561 accgatagga atgcgccgca gccgagcttc tgcagtgcct tgatgccgtg ggtaagtaca
     4621 gtgtagccgc tgcgcctagc gatcgcctcg gcctccagtc gcagcgcctt cggcgtcagc
     4681 acgttgggcg ccattgccgt cagccgtcgc gccagcacgt tgccgtcggc gattgcgcgc
     4741 accgcctggt gatcgggctg cttaccattc cagacgatcg ccgccagcgg cttgcgctcg
     4801 ggctcggcgc gcgcgctcgg cagcgtcacg ccgttgatcc agcagacgta gagcgtctcg
     4861 ttggcggcgc tctcgggcag gccggacgaa agcgacacgc tgaggcgctc ggctccgtcg
     4921 ccgagcagga tcgccacggc cttgcgcaag gccgtgaaac gctcgaagcg cgaggcgcgc
     4981 gaatcccagc gaatccagac gatcaatcca cccttgtcgt cggtcgccgc gatcggcgtc
     5041 gtcgacaaat cggccatcgt cttgcgtcgc cgccgcagca cattgcgcag caactcatgc
     5101 tcatcgggga cggccgcgac ggaagccttt gcggaaattc cgacggcatt gtcgccgtcg
     5161 ataatgcaga gccggtgcat gacccgcgac gccttaccgc gcggggcctt tccggggaat
     5221 tcgaggttcg ccagcatgct atagtctgcg gcctgtttag gccggtccgt cgccacgttc
     5281 cgccgattat agccaccgca agccatgcat ccctacctca acctgatgcg ccacgtgctg
     5341 gagaacggcc accgcaagag tgaccgcacg ggcaccggca ccctttcggt attcggctgg
     5401 cagatgcgct acgcgctcga cgaa
//
LOCUS       DATDAA010000028         5129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_491307,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000028 DATDAA010000000
VERSION     DATDAA010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491307"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VIS73_00990"
     CDS             complement(<1..427)
                     /locus_tag="VIS73_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEY5761761.1"
                     /translation="MAHPTAQLVLGSSSPFRRELLARLGIPFAVLSPDIDETARASEA
                     PRELALRLAGEKARAVARSHPAGLIIGCDQVAHIGTRVFGKPGTHQNAARQLAELSGQ
                     TVVFESALCLLDASTGRMQIDSIPTEITFRTLSAPEIENY"
     gene            501..956
                     /locus_tag="VIS73_00995"
     CDS             501..956
                     /locus_tag="VIS73_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEY5761762.1"
                     /translation="MEFAKSGRQLDGRLGLAELTRAGDLLVAADLEYRLSGSVGRRGG
                     FQLRLRLAGSVGLRCERCLETVMLPIEQDSLFELRRSEGDIRPDDLLDDERDFLVFDR
                     AMVVTDLVEDELILCIPVAPKHTDCEPAGSMVKDEGRRQPFGVLASLKK"
     gene            1002..1181
                     /gene="rpmF"
                     /locus_tag="VIS73_01000"
     CDS             1002..1181
                     /gene="rpmF"
                     /locus_tag="VIS73_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY5761763.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDFLTNPPLAVEPTTGEVHLRHHVSP
                     SGVYRGKKVIKAKGE"
     gene            1247..2269
                     /gene="plsX"
                     /locus_tag="VIS73_01005"
     CDS             1247..2269
                     /gene="plsX"
                     /locus_tag="VIS73_01005"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY5761764.1"
                     /translation="MSTTIAVDCMGGDHGPSVTVPAALDFLESHPDAEVILVGREDAI
                     RPHLPVTAKRWSIRHASEVVEMDDPPAIAMRTKKDSSMRVAINLVKEGAAGASVSAGN
                     TGALMAISRFVLKTLPGIDRPAICTMLPTYKGHTHVLDLGANVDCEPEHLLQFGIMGA
                     GLVSAVDGIERPTVGLLNIGEEEIKGNETVKAAAELLRQSGLNFIGNVEGDGIYFHGG
                     ADVVVCDGFVGNVALKVSEGLAQMLSTGLREEFSRSWMAKLGALAAMPVLKRFKQRID
                     HRRYNGASLLGLRGVVVKSHGSADRFAYRHALARAYDAASHDVLQRIEKQLSVPAASA
                     SVMEDA"
     gene            2269..3231
                     /locus_tag="VIS73_01010"
     CDS             2269..3231
                     /locus_tag="VIS73_01010"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY5761765.1"
                     /translation="MSFARLTGVGSQLPDQCVSNDDLVARGVDTNDEWVVSRTGIRTR
                     YLAAEGQATSDLAADAARKALNNAGVAADAVDLIIVATSTPDQVFPSTACVLQRKLGN
                     RSATAFDIQAVCSGFVYALSIAEKFIQSGTHRCALVIGAEVFSRILDWNDRATCVLFG
                     DGAGAVVLQADNKPGILASALHADGSYSGILSVPGQVQAGQVVGDPFLRMDGQAVFKL
                     AVRVLAEVAQEVCAAAGRSTDSIRWLIPHQANVRILDATAKKLGVDPSRVVVTVDRHA
                     NTSAASVPLALDVAVRDGRIQRGDSVLLEGVGGGFTWGAVLLDF"
     gene            3244..4173
                     /gene="fabD"
                     /locus_tag="VIS73_01015"
     CDS             3244..4173
                     /gene="fabD"
                     /locus_tag="VIS73_01015"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY5761766.1"
                     /translation="MSFAFVFPGQGSQSVGMMTAYGDAPVVRQTFEEASAALGEDLWQ
                     MVVEGPAERLAQTVNTQPVMLAAGVAVWRLWQAKGGRAPAVMAGHSLGEYAALVAAGA
                     LAFTDAVPLVRLRAQAMQEAVPVGTGAMAAVLGLDDEQIAAACVEAANGEVVEAVNFN
                     AVGQTVIAGHKAAVERAIEACKARGAKRAISLPVSAPFHSSLMRPAAERLGERLKAIE
                     IRPPTIPVINNVDVAVESDPARIKDALVRQAFSPVRWVEIVRRMSAMGVTTTAECGPG
                     KVLAGVAKRCADGVSGLALADEAALVAATSNLE"
     gene            4178..4918
                     /gene="fabG"
                     /locus_tag="VIS73_01020"
     CDS             4178..4918
                     /gene="fabG"
                     /locus_tag="VIS73_01020"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY5761767.1"
                     /translation="MLNGQVALVTGATRGIGRAIALKLGSLGATVVGTATSESGAEAI
                     SEALACAGIAGRGMVLNVTDWNAADALIDSVAKEFGAIAILVNNAGITRDNLALRMKD
                     DEWDAVIDTNLKAVFRMSRAVMRGMMKARVGRIINITSVVGHAGNPGQPNYCAAKAGV
                     AGLSRSLARELGSRNITVNCVAPGFIQTDMTDALNEAQRTALLATVPLARLGTPEDVA
                     DAVAFLASPAASYVTGNTIHVNGGMYME"
     gene            4999..>5129
                     /locus_tag="VIS73_01025"
     CDS             4999..>5129
                     /locus_tag="VIS73_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY5761768.1"
                     /translation="MENIEQRVKKIVAEQLGVNEADIKIESSFVDDLGADSLDTVELV
                     "
BASE COUNT          909 a         1510 c         1735 g          975 t
ORIGIN      
        1 ggtagttctc gatttcgggc gcgctcaggg tgcggaaggt gatttcggtc gggatcgagt
       61 cgatctgcat ccgtcctgtg ctcgcgtcga gcaggcacag cgctgattcg aacacgacgg
      121 tctggcccga cagttcagcg agctggcggg cggcgttctg gtgggtgccg ggcttcccga
      181 atacccgggt accgatgtgg gcgacctggt cgcaaccgat gatcaggccg gccggatggc
      241 ttcgggcgac ggcccgcgcc ttttctccgg ccaggcgtaa cgcaagctcg cggggtgctt
      301 cgctggcccg tgcggtctcg tcgatatcgg gtgagaggac cgcgaagggg atgcccaggc
      361 gggcaagcag ttcacggcga aacggagagc ttgatccgag gacaagctgg gcggtcggat
      421 gtgccacggc attctttgac aggggaagcc ggaaatatta ccatcgcgcc cctatgtcaa
      481 agggcggtgc catcgataac gtggaattcg cgaaatcggg gcgccagttg gacggtcgtc
      541 tgggtctcgc ggagttgacg cgcgcagggg atctgctggt tgccgcggat cttgaatacc
      601 gcctttcggg gtcggtcggt cgccgcggcg ggtttcagct gaggttgcgc ttggcaggca
      661 gcgtcggctt gcgctgcgag cgttgtctgg aaacggtgat gctaccaatc gaacaggaca
      721 gcctgttcga attacgtcgc agtgaaggcg atatccgccc cgatgacctg cttgacgatg
      781 aacgcgattt cctggtgttt gaccgagcca tggtggtcac tgatctggtc gaggacgaat
      841 tgattctttg cattccggtg gcgccgaaac acacggattg tgaacctgcc ggcagcatgg
      901 tgaaggacga gggccgccgg caacccttcg gtgtcttggc ctcgttgaaa aaatgaggcg
      961 cacgggatct gcccggcgcc agtacttgat aggagcatca aatggctgtt cagcaaaaca
     1021 agaagtcgcc gtcgaagcgc ggcatgcacc ggtcgcatga ctttctgaca aatccgccgc
     1081 tcgcggtcga gccgaccacc ggcgaagtgc acctccgcca ccacgtgagc ccgtcgggcg
     1141 tctatcgtgg taagaaggtc atcaaggcta agggcgagta attctccgga cccgcaaggc
     1201 aggcaggcat taacgctgcc tgcctttttc tttggctgaa ctgaacatgt cgacaacgat
     1261 agccgtggac tgcatggggg gcgaccatgg cccctcggtc accgttccgg cggcgctgga
     1321 ttttctggaa agccatcccg acgcggaagt gatactcgtt ggccgcgagg atgccattcg
     1381 tccgcatctg ccagtgacag cgaaacgctg gtcgattcgc catgccagcg aggtcgtcga
     1441 gatggacgac ccgccggcga ttgccatgcg cacgaagaag gactcgtcga tgcgcgtcgc
     1501 gattaatctg gtcaaggaag gcgcggctgg tgccagcgtt tcggctggca ataccggcgc
     1561 gctgatggcg atttcccgct tcgtgctgaa gaccctgccc ggcatcgacc ggccggcgat
     1621 ctgcacgatg ctgccgacct acaaggggca tactcatgtc ctcgatcttg gcgccaacgt
     1681 cgattgcgaa cccgagcacc tgctgcagtt cgggatcatg ggtgccggtc tggtgtcggc
     1741 ggttgacggc atcgagcggc caacggtcgg actcctcaac atcggcgaag aggaaatcaa
     1801 gggcaacgaa acggtcaagg cggctgccga actgctgcgt caaagtggcc tgaacttcat
     1861 cggcaacgtt gagggcgatg gcatctattt ccatggcggc gctgatgtcg tcgtctgcga
     1921 cggcttcgtc ggcaacgtcg ctttgaaagt gtcggagggt ctggcgcaga tgctgtcgac
     1981 cggactgcgc gaagagtttt cgcgcagctg gatggccaag ctcggtgcgc tcgccgcgat
     2041 gcccgtgctc aagcgtttca agcagcgaat cgaccatcgg cgctacaacg gcgcgagcct
     2101 gcttgggctg cgcggcgtgg tcgtcaagag tcatggctcg gccgatcgct tcgcctatcg
     2161 ccatgcgctg gcgcgcgcct acgacgcggc gagccacgac gtcctgcaac gaatcgagaa
     2221 gcaactgtcg gtaccggcag catccgcctc ggtcatggag gacgcgtgat gagctttgcg
     2281 cgcctgaccg gtgtcggcag ccagttgccg gatcaatgcg tcagcaacga cgatctggtg
     2341 gcgcgtggcg tcgataccaa cgacgaatgg gtggtttccc gtaccggaat ccgaacacgt
     2401 tacctggcgg ccgaggggca ggcgaccagc gatctggcgg ctgacgcggc gcgcaaggcc
     2461 ctcaataatg ccggcgttgc agccgacgcg gtggacctga tcatcgtcgc gacgtcgacg
     2521 cccgatcagg tcttcccgag tactgcctgc gtccttcagc gcaagctggg taaccgctcg
     2581 gctacggcct tcgacatcca ggcagtctgc tcgggcttcg tctacgcgct gtcgattgcc
     2641 gagaaattca tccagtcggg tacgcaccgc tgcgcgctgg tcatcggcgc cgaagtgttc
     2701 tcgcggatcc tcgactggaa cgatcgagcc acctgtgtac tgttcggcga cggcgcgggc
     2761 gccgtggttc tccaggccga taacaagccc ggcattctcg cttcggcgct acatgccgac
     2821 ggcagttact cgggaatcct gtcggtcccc ggtcaggtgc aggcggggca ggtcgtcggc
     2881 gatccctttc tgcgaatgga tggccaggcc gtattcaagc tcgctgttcg cgtactcgcc
     2941 gaagtcgcac aggaagtctg cgctgccgcc ggacgatcga ccgactcgat acgctggttg
     3001 attccgcatc aggccaacgt gcgcatcctt gacgcaaccg ctaagaagct cggcgtcgat
     3061 ccatcgcggg tggttgtcac ggtggatcga catgccaata cctcggcggc atcggtgccg
     3121 ctcgcacttg atgtcgcggt tcgcgacggc cggatccaac gtggtgacag cgtgctgctc
     3181 gaaggggttg gcggtggctt tacctggggc gcggttcttc tggatttctg atcggagtcg
     3241 atgatgagtt ttgctttcgt attccccggg caggggtcgc aatcggtggg catgatgacc
     3301 gcctatggtg atgcacccgt ggtgcgccag acctttgaag aggcatcggc cgcgcttggt
     3361 gaagacctct ggcagatggt tgtcgaaggg ccggccgagc gacttgcgca gaccgtcaac
     3421 acgcaacccg tgatgcttgc ggcgggcgtt gctgtctggc gcctctggca ggcaaagggt
     3481 ggccgggcgc cagcggtgat ggctggccat tcgctcggcg aatacgccgc gctggttgcc
     3541 gccggtgcgc tggcctttac cgacgcggtt ccgctggttc gcctgcgtgc gcaagcgatg
     3601 caggaagccg tgccggtcgg taccggcgcg atggcagcgg ttctcggact cgacgacgag
     3661 cagatcgcgg ctgcctgtgt cgaggcggcg aatggcgagg tggttgaagc ggtcaacttc
     3721 aacgcagtgg gacagacggt catcgccggt cacaaggcgg cggtcgaaag ggcgatcgag
     3781 gcgtgcaagg cgcgtggcgc caagcgtgca atttcgctgc cggtctcggc accattccac
     3841 tcctcgctga tgcggcccgc tgccgagcgg ctcggcgagc gactaaaggc aatcgagatc
     3901 cggccgccga cgattccggt catcaacaac gttgacgtgg ccgtcgagag cgaccccgcg
     3961 cgcatcaagg atgcgctggt ccggcaggca ttctcgcccg tccgttgggt cgagatcgtc
     4021 cgtcggatgt cggcgatggg tgtgacgaca accgccgagt gtgggccggg caaggtactg
     4081 gcaggtgtcg ccaagcgctg cgccgatggc gtcagcgggc tggcgctggc cgacgaagcg
     4141 gcgctggttg ccgcaacaag caacctggag taatcaaatg ctgaatggac aagtggcgct
     4201 ggttaccggc gcgacccgcg ggatcggtcg ggcaatcgca ctgaaactcg gttcgctcgg
     4261 cgcgacggtg gtaggcacgg cgaccagcga gagcggggcc gaggcgatca gcgaagcgct
     4321 ggcctgcgcc ggcattgcgg gtcgcggcat ggttctcaac gtaaccgact ggaatgcggc
     4381 cgatgccctg atcgattcag tagcaaagga gttcggggcg atcgcgattc tggtcaacaa
     4441 cgccggcatc acgcgcgaca acctggcgct gcggatgaag gacgacgagt gggacgcagt
     4501 catcgacacc aacctgaagg cggtatttcg catgtcgcgg gctgtcatgc gcggcatgat
     4561 gaaggcgcgg gtcggccgca tcatcaatat cacctcagtc gtcggccatg cggggaatcc
     4621 cggccagccg aactactgcg cggccaaggc cggcgtcgcc gggctgtctc gttcgctcgc
     4681 ccgcgaactg ggcagccgca atatcaccgt caactgcgtc gcgcccggct tcatccagac
     4741 cgacatgacc gacgcgctga acgaggcgca gcgcaccgcg ctgcttgcaa ccgtgccgct
     4801 ggcgcgcctc ggtacgcccg aggatgtggc cgacgcggtt gccttcctcg cgtcgcctgc
     4861 ggcctcctac gtaacgggca acacgattca tgtcaatggc gggatgtaca tggagtaatt
     4921 ccggggattg ccgtgccttg taaggccctt tccttctgat agaatcccgc cttctttttg
     4981 tgccacagga gtttttgaat ggaaaacatc gaacagcgtg tcaagaaaat cgtcgccgag
     5041 cagttgggag tcaatgaggc ggacatcaag attgagtcct cctttgtcga cgacctcggc
     5101 gcggactcgc tggataccgt cgaattggt
//
LOCUS       DATDAA010000029        12449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_386609,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000029 DATDAA010000000
VERSION     DATDAA010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12449
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386609"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VIS73_01030"
     CDS             complement(<1..846)
                     /locus_tag="VIS73_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5761769.1"
                     /translation="MEALSVATSAADTRRLARERPPPLVVDGLIKRYGSFTAVDGVSF
                     RATSSRCFGLLGPNGAGKTTTLRACLGLTEPDAGEISLLGRPVPARAREARQYTGIVP
                     QFDSLDPDFTVAENLRVYGRYFGLSDRAVRAQIPDLLEFAGLAGKADAPLKNLSGGMK
                     RRLTLARALVNDPEIVFLDEPTTGLDPQARHLIWDRLRKLTASGKTLVLTTHFMDEAE
                     RLCDRVVIMAEGRAMCDGAPAELITRYLRREAVELDCPEDAEAALLDGLGHGVVTLRA
                     GDRLMV"
     gene            complement(834..1472)
                     /locus_tag="VIS73_01035"
     CDS             complement(834..1472)
                     /locus_tag="VIS73_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5761770.1"
                     /translation="MRLETTQTLIDGPAGAIDTIVETPHTPKGIAIVAHPHPLFGGAN
                     NNKVAQTLARTLGNLGYVAFRPNFRGVGKTEGAHDEGRGETEDLLAVLDHARAAFGEL
                     PVVLAGFSFGAYVQARVAHRLRDAGTPPQRLILVGTAAGHVEGTRSYDTEAVPSDTIV
                     IHGSIDETVPLANVLAWAEPLDLPVVVVPGADHFFHRRLHVIRDIITRAWKH"
     gene            complement(1552..1860)
                     /locus_tag="VIS73_01040"
     CDS             complement(1552..1860)
                     /locus_tag="VIS73_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEY5761771.1"
                     /translation="MSHFKHHVFFCCNQREAGDSCCNNHGATTMQAYAKDRIGALKLK
                     GKGKVRINKAGCLDRCDDGPVLVIYPEAVWYTYVDQADIDEIIDEHLVNGRVVERLQI
                     "
     gene            complement(1886..2971)
                     /locus_tag="VIS73_01045"
     CDS             complement(1886..2971)
                     /locus_tag="VIS73_01045"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY5761772.1"
                     /translation="MPDSLAPRPTRLAGGLALSAAALIAAGSLYPFSGWQSNGLPPWA
                     FLFSGWPRYWTGNDLAVNVLAYLPLGTLVGIGLSRRLRSLPAALAAWLSCVLLSLVVE
                     MLQHYLPSRIPSQFDLICNAAGAALGVGLATWRARPLLAIEDRLATVLARVPHPAYGL
                     ALLALWLLTQLSADAVFGTTGDLRRIFGAPAAGALSLALPLEALAVACHLVLVGLLAN
                     RLFGSNRPALPSIVLCLVSAAACATAARAIWLEPAHAFDWLSPSTISGLLAGSVALLL
                     VLLLPATAMRVAATVALGLGVAAINLLPVHPYQTALAPWQLGAYLNINGLTRWLGVVW
                     PLFALVWLIAGPWQRSRWHETVFRRSL"
     gene            complement(2995..4461)
                     /locus_tag="VIS73_01050"
     CDS             complement(2995..4461)
                     /locus_tag="VIS73_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEY5761773.1"
                     /translation="MLLWLVIAYLVVSIGIGLYAATRVHNARDYITAGRNLPMVVVIA
                     MVFATWFGAETVLGISATFLDEGFRGLISDPLGASVCLILFGLVFARPLYRMNLLTLG
                     DFFRVRYNRSTELILSTCIVLSYLGWVAAQITALGLVFNVLSQDAVPMNQGMLIGAGV
                     VLVYTLFGGMWSVAMTTFVQMIVIVLGLLYVTFLAGDMAGGFGTVVGKAAAEGKLSFL
                     PTLDALDMIAWIAAFATMALGSIPQQDVFQRVNSSKNETIAVWGTTLGGASYFFFAAV
                     PLFLAYTATIIDPAMVSALMEQDSQLILPTLVLQYMPFAAQVIFFGALLSVIMSTASG
                     TLLAPSVTFSENVLRGFLPNMSDSQFLWATRATVVVFTIMVVTYATSTESTIHQMVEN
                     AYRITLAGAFVPLAAGLFWKRANNLGAALAIVLGLFTWLMLELFVPEGDVEPQLIGLL
                     ASAIGMLAGGYLGTPNSEPQARPGKSHQAAAMTHHVPH"
     gene            complement(4620..5420)
                     /locus_tag="VIS73_01055"
     CDS             complement(4620..5420)
                     /locus_tag="VIS73_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761774.1"
                     /translation="MNLPTTVRSAAWLAAFAAMPAMAGLSAGTGFDYTSGDYGTDTTT
                     TILTIPLIVKYDEGPLVLKATLPYVRAEGKTSGALEFDTRRSGTEVEVRQLPATAVRQ
                     SGFGDLTASAFLNVWNSQAGHGLDLGIKAKIATTASSRDFLITSGENDYSIQADYFMP
                     LRGVANSSLFFTFGHTLKGDPPGLDYRDPNYFSAGFSQRVSETGTWGVAYDFREKLLA
                     GRDPVSEGSLFYSYKYSKDWKVQTYGVVGIASDSSPDFGLGATITHAY"
     gene            complement(5504..6721)
                     /locus_tag="VIS73_01060"
     CDS             complement(5504..6721)
                     /locus_tag="VIS73_01060"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEY5761775.1"
                     /translation="MRHILPFFLAAATALSATPVLAAAACAEGPAGGIGGTSAPARGG
                     IGGTGSPAELALGGIGGTGQVVLPIAAGETGAVVGTITGFASICVNGLEVHYEDSTPV
                     TANGAGSSTHSLAIGQVVAIEATPSARGLEAKRVAIVHAVVGPVTRVDGKNVEVMGQR
                     VVRSDAADALAIGDTVRVSGLRDADGAIVASRIDRAESSEIEHSVIGRVRDGRIEGLR
                     VEAVELPEGAEVVVRGRFGDGRLQASRVERSPAREYTERARAVSIEARITERSGRRLS
                     AGGRTVELGDDTRVVGGRDDEADRNRLIRITGRVDKDGKVRAERIEIRDRREERPRRE
                     GSSGLDDSPDGREDHRGGDGRSANSGRDRSGRSESPEREDRSGQGSGERYERAERAER
                     VERPERIERPDHD"
     gene            complement(6732..7589)
                     /locus_tag="VIS73_01065"
     CDS             complement(6732..7589)
                     /locus_tag="VIS73_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HEY5761776.1"
                     /translation="MTSPTANATPDLLLKALRRALRPLVRLMLSKGVTYLQLTELLKG
                     LFVEVADRDFPVPGKAQTDSRISLLTGIHRKDVKRLRAEILPEAPMPETISLGMRLAA
                     AWGEPPFADEEGNPLPLPRLSSQSQSVSFESLVASVSKDIRARAVLDEWLRLGVVVLD
                     DEDRVCLKAGAFVPQRGFEEKAFYLGLNLHDHAAAIADNLEIEEGASPWLERCVHYDE
                     VHADDLAQLRKDAENGTMRLLRNINRKAADSEARAAAGDGPRKRLTLGVYFYADDAER
                     RDHNDGERR"
     gene            7710..8450
                     /locus_tag="VIS73_01070"
     CDS             7710..8450
                     /locus_tag="VIS73_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761777.1"
                     /translation="MRQKTGGATNQKEGRTARSGPTMKEKQMKTKQMIPALLATMFLA
                     VGAVQAQSTSDDIVGGVKLRGDGTVDNTQPGAVGGGNDIVDGVKRRGDGSVDDSQPGA
                     PSGNDDIVNGVKRRGDGSVDDSQPGMASGSDDIVNGVKRRGDGSIDDNSIPGSETGDD
                     RRSDRAKEVLSRDAKERRSERAKSRIEDNRERHETRVKSREVVDGVRVRTETRERTEV
                     RDRGERAERAERTERVERAEREERSGRD"
     gene            8521..9939
                     /locus_tag="VIS73_01075"
     CDS             8521..9939
                     /locus_tag="VIS73_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5761778.1"
                     /translation="MAKNEATAATKPRRRTDGRSQRFDLVRWFSWVALVAGTAVTLSA
                     AFSLSAFLTSRMVAQEASLTADFVRSVIAADDAYRVFAQPESATAEDRIRAVDVFTHL
                     AEMPDVIRTNVYAADGTVLWSSDAEMIGRRFAENDELKEALRAEVVVHSGVVDRETLT
                     KPEHRNLDLRHERFVESYTPIFAKNSREVIGVVELYRIPEQLFGAIHDGQVLIWSGAV
                     ITAIFLFVAFSGIVAKAQQVIERQHEQLIEQEGLAVVGEMGSAVAHGLRNPLASIRSS
                     AEVALGSDIPADARECANDIISQVDRLEGWIRRLLTYAKPSTAALGPVTIDRVVNECV
                     GDYRRELEKRDIQLLVGVDAGLPPVRGEAGILGQLINSLIVNAAEAIQGPGKIQVSAR
                     RHAEGVMVEVLDDGPGLNDPDIEKVFTPFYTTKQKGLGLGLPLVKRVIERFGGTVAIT
                     NTQPRGVSVRLFLQTHPQEVQS"
     gene            9936..11348
                     /locus_tag="VIS73_01080"
     CDS             9936..11348
                     /locus_tag="VIS73_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY5761779.1"
                     /translation="MSDAILIVEDEAILAKNIRIFFERAGYDARVAQSAEDALMELDD
                     FRPDLVLLDFQLPGINGLEMLELLREREPQLPVVMLTGQGTVELAVRAMRSGAVDFLS
                     KPVALQHLRVVVDRLLDERRNDTASQYYRARSATGVAALIGESQPMRQLKETIRTILE
                     AEATMTDAAPPTILVTGETGTGKELVARALHCDGPRSDGPFIELNCATVPAPLLEAEL
                     FGYERGAFTDAKERKIGLVEAATGGTLFLDEIGDMDPALQAKVLKLLEEKVVRRLGAI
                     RDQKVDVRIIAATHQPLDQLVAQGKFRADLYFRLRMVQIELPPLRERGDDILLLAKEF
                     LAQQSLRYRKQGLSFSQDAQTALRNHAWIGNVRELRNLIEQTVLLASDPIVAAKQLAF
                     YLPSKVVPTDLEQPEKGRRASDLVVGDEIRPLSEVERGLLVEALHKTSWNVTRAARLL
                     GISRDTLRYRIEKHNLSRYS"
     gene            complement(11352..>12449)
                     /locus_tag="VIS73_01085"
     CDS             complement(11352..>12449)
                     /locus_tag="VIS73_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEY5761780.1"
                     /translation="REVAVKVLRPGMLAAIENDLALLDTFAWLAETLWSDGKRLKPRE
                     VVAEFAKHLHDELDLMRESANCSQLRRNFAESTLLRVPEVYWDWCSSSVMTMERMHGT
                     PISQIDRLRSQGIDLHRLGSAGVEIFFTQVFRDGFFHADMHPGNIFVSPEGQYIALDF
                     GIMGTLTDVDKNYLAQNFLAFFKRDYKRVALAHIEAGWAPRETRPDEFEAAIRAVCEP
                     IFDRPLREISFGRVLLRLFQTSRRFNVEVQPQLVLLQKTLLNIEGLGRQLDPELDLWK
                     TAKPYLEQWMSEQVGWRALIAALRNESPNWAKSLPQLPRLAHQALSRPPDSAQRETLE
                     RIAAAQQVQNRLLAILIIVIALLAATPWLLR"
BASE COUNT         2286 a         3910 c         4180 g         2073 t
ORIGIN      
        1 caccatcagc cggtcgccgg cgcgcagcgt gacgacgccg tggccgaggc cgtcgagcag
       61 cgccgcctcc gcgtcctccg ggcagtcgag ctcgaccgcc tcgcgcctga gatagcgggt
      121 gatcagctcg gccggcgcgc cgtcgcacat ggcgcgtccc tcggccatga tcacgacgcg
      181 gtcgcacagc cgctcggcct cgtccatgaa atgcgtggtc agcaccagcg tcttgcccga
      241 ggccgtcagc ttgcgcaggc ggtcccagat caggtggcgc gcctgcggat cgagcccagt
      301 ggtcggttcg tcgaggaaga cgatctccgg gtcattgacc aaggcgcgag ccagcgtcag
      361 ccggcgcttc atgccgcccg aaaggttctt cagcggcgcg tcggccttgc cggcgaggcc
      421 ggcgaactcg agcaagtcgg gaatctgcgc gcgcaccgcg cggtcgctga ggccaaagta
      481 acggccgtag acccgcaggt tttcggcgac cgtgaagtcg gggtcgagac tgtcgaattg
      541 ggggacgatt ccggtgtact gcctggcttc gcgggcccgt gccggcaccg gccggccgag
      601 cagcgagatc tcgcctgcat cgggctcggt cagaccgagg caggcgcgca gcgtcgtggt
      661 cttgcccgcg ccgttggggc cgagcaggcc gaaacagcgg ctcgaggtag cgcggaaact
      721 gacgccgtcg accgcggtga agctgccata gcgcttgatc agaccatcga ccaccaacgg
      781 cggcggccgc tcgcgggcta gtcgtcgggt gtcggccgcg ctcgtcgcga cgcttagtgc
      841 ttccatgcgc gggtgatgat gtcgcggatc acgtgcaggc ggcggtgaaa gaaatgatcg
      901 gcgccgggaa ccacgaccac gggcaggtcg agcggctcgg cccaggccag cacatttgcc
      961 agcggtacgg tctcgtcgat cgagccgtgg atgacgatgg tgtccgacgg cacggcttcg
     1021 gtgtcgtagc tgcgcgtgcc ttcgacgtga ccggcggcgg tgccaaccag aatcaggcgt
     1081 tgcggcggcg tgccggcgtc gcgcaagcga tgcgcgacgc gtgcctgcac ataggcgccg
     1141 aaagaaaacc cggccagcac cacgggcagc tcgccgaagg cggcacgcgc atggtcgagt
     1201 acggcgagca ggtcctcggt ctcgccgcgc ccctcgtcat gcgctccctc ggtcttgccg
     1261 acgccgcgga agtttggccg gaaggcgacg tagccgaggt tgccgagcgt ccgcgccaag
     1321 gtctgcgcga ccttgttgtt gttggcgccg ccgaacagcg ggtgcgggtg ggcgacgatg
     1381 gcgatgccct tcggcgtgtg cggcgtttcg acgatcgtat cgatcgcgcc ggctgggccg
     1441 tcaattagcg tttgtgtggt ttcgagtcgc atggtccatc agacaggcaa gggtggtgac
     1501 ggcgggcggg atggccgaac cgccggctcc ccgcccgatt gatcgacagc gtcagatctg
     1561 cagtcgttcg acgacgcggc cattgacgag gtgctcgtca atgatttcgt cgatgtccgc
     1621 ctggtcgacg taggtgtacc agaccgcctc gggatagatc accagaaccg ggccgtcgtc
     1681 acagcggtca agacagccag ccttgttgat acggaccttg cccttgccct taagcttgag
     1741 cgcgccgatc cggtccttgg cataggcctg catcgtcgtc gcgccgtggt tgttgcagca
     1801 gctgtcgcca gcctcgcgct ggttgcagca aaagaagacg tggtgcttga aatggctcat
     1861 ggcgggacct cggtggggtg ggcgattata acgagcggcg aaaaaccgtc tcatgccagc
     1921 ggcttcgctg ccagggtccg gcgatcagcc agaccagcgc gaacagtggc cagaccacgc
     1981 cgagccagcg cgtcaggccg ttgatattga ggtaggcgcc gagttgccag ggtgccaagg
     2041 ccgtctgata ggggtggacc ggcaacaggt tgatcgccgc aacccccagt cccagcgcga
     2101 ccgtcgcggc gacgcgcatg gccgtcgctg gcagtagcaa gaccaggagc agggcaacgc
     2161 tgccggccag cagtccgctg atcgttgatg gtgagagcca gtcaaaggca tgcgccggtt
     2221 ccagccagat tgcgcgcgcc gccgtggcgc aggccgccgc ggaaacgagg cagagcacga
     2281 tcgacggcag cgccggtcgg ttgctgccga acaggcggtt ggcgagcagg ccgacgagga
     2341 ccaaatggca ggcaacggca agcgcctcca gtggcaatgc aagcgacagc gcgccggccg
     2401 ccggtgcgcc aaaaatccgc ctcaggtcgc cggtcgtgcc gaacacggca tcggccgaca
     2461 attgggtcag caaccacagc gccagcagcg ccagcccgta ggccgggtgc ggcacccgtg
     2521 cgagcaccgt cgccagtctg tcctcgatgg ccagcaaggg gcgggcgcgc caggtcgcga
     2581 gccccacacc gagtgcggcg ccggccgcat tgcagatcag gtcgaactgc gagggaatcc
     2641 ggcttggcag gtaatgctgg agcatttcga cgaccaggct aagcaggacg caggacagcc
     2701 atgccgccag cgccgccggc agactgcgca agcgccggga gaggccgatg ccgaccaggg
     2761 ttcccagcgg caggtaggcg aggacgttga cggcgagatc gttgccggtc cagtagcgcg
     2821 gccagccaga aaagaggaag gcccagggtg gcaagccgtt gctttgccag ccgctgaacg
     2881 ggtagaggct gccggcggca atcagcgcgg ccgccgacaa cgcgagcccg ccggccagtc
     2941 tggtcggtcg gggcgcgagc gaatcgggca atgtgtgggc gcctgactcg tggctcagtg
     3001 cgggacgtga tgagtcatcg ccgccgcctg gtggctcttc cctgggcgcg cctgcggttc
     3061 ggaattcggc gtgccgaggt agccgccggc gagcatgccg atcgcgctgg cgaggaggcc
     3121 gatcagctgc ggttcaacgt cgccttccgg cacgaacagt tcaagcatca gccaggtgaa
     3181 cagcccgagc acgatcgcca gcgccgcacc gaggttgttg gcccgcttcc agaagaggcc
     3241 tgccgcgagc gggacgaagg cgcctgccag agtgattcgg tacgcattct cgaccatctg
     3301 gtggatcgtc gattcggtgc tcgttgcata ggtgacgacc atgatcgtga atacgaccac
     3361 cgttgcccgc gttgcccaga ggaattgcga atcgctcatg tttggcagga agccacggag
     3421 tacgttctcg gaaaaggtca ccgaaggggc gagcagggtg cccgaggccg tgctcatgat
     3481 caccgagagc agcgcgccga aaaagatcac ctgcgcagca aagggcatgt actggagaac
     3541 gagggtgggc aggatcaatt gcgaatcctg ctccatcagc gcggatacca tcgccgggtc
     3601 gatgatggtc gcggtatagg cgaggaagag cggcacggcg gcgaagaaga agtagctggc
     3661 gccgcccagc gttgtgcccc agaccgcgat cgtttcgttc ttcgacgagt tgactcgctg
     3721 gaagacgtcc tgctgcggaa tcgagccaag cgccatcgtc gcgaaggcgg cgatccaggc
     3781 gatcatgtcc agcgcatcga gcgtcggcag gaacgacagc ttgccctcgg cggcggcctt
     3841 gccgaccacg gtgccgaagc cgccggccat gtcgccggcc agaaaggtca cgtagagcag
     3901 cccgaggacg atgacgatca tctgcacgaa ggtggtcatc gccaccgacc acatgccccc
     3961 gaacagcgtg tagacgagga cgacgccggc gccgatcagc atgccctggt tcatcggcac
     4021 ggcgtcctgc gagagcacat tgaataccag gccgagtgcg gtgatctgcg cggcaaccca
     4081 gccaagatag gaaagcacga tgcaggtcga caggatcagc tccgtgctgc ggttgtagcg
     4141 gacgcggaag aagtcgccga gtgtcagcag gttcatccga tacagcggcc gggcgaagac
     4201 caggccgaac aggatcagac agaccgaagc gcccaacgga tcggaaatca acccgcggaa
     4261 gccctcgtcg agaaaggtcg ccgagatccc cagaaccgtc tcggcgccaa accaggtggc
     4321 gaagaccatg gcgatgacca ccaccatcgg cagattgcgg ccggcggtaa tgtagtcgcg
     4381 ggcgttatgg acgcgggtcg cggcgtagag gccgatgccg atcgatacga cgaggtaggc
     4441 aatgacgagc cagaggagca tggaagcctc cgcaagggaa accccgcggc ggcggggtta
     4501 ttcgctgttg atcgggttgg cgtccgcggt cgaacggggc aggatcgcca gtcgcggcga
     4561 ttatacaaac gaaaagcgcg gccaattccg gccgcgctgt cgtcaattgc tccggcggat
     4621 caataagcgt gggtaatcgt cgcgccgagg ccgaaatcag ggctcgaatc cgaggcgatt
     4681 ccgactacgc cgtaggtttg caccttccag tccttgctgt acttgtacga gtagaacagc
     4741 gatccctccg agaccgggtc gcgtccggcc agcagctttt cgcggaagtc ataggcgacg
     4801 ccccaggtgc cggtctccga gacgcgctgg ctaaagccgg ccgagaagta gttcgggtcg
     4861 cgatagtcga ggcccggcgg atcgcccttt agcgtatggc caaaggtgaa gaacagcgac
     4921 gaattggcca cgccgcgcag cggcatgaag taatcggcct ggatcgaata gtcgttctcg
     4981 cccgaggtga tcaggaagtc gcggctgctt gcggtggtcg cgatcttcgc cttgatgccc
     5041 aagtccaggc cgtgtcctgc ctggctgttc cagacgttga ggaaagcgct ggcggtgagg
     5101 tcgccgaagc cggactgtcg gaccgccgtc gccggcagct ggcggacttc gacttcggtg
     5161 ccgctgcgcc gcgtatcgaa ctccagcgcg ccgctggtct tgccttcggc gcgcacgtag
     5221 gggagcgtcg ccttgagcac cagcggacct tcgtcgtact tgacgatcag cggaatcgtc
     5281 agaatcgtcg ttgtcgtatc ggtgccgtag tcgcccgacg tgtaatcgaa gccggttccg
     5341 gcagacaggc cggccatggc cggcatggcg gcgaaggctg ccagccaggc ggcgctgcga
     5401 acggtggtgg gcaaattcat gcgcatttct ccaaaacggt agataggggt tggcagttga
     5461 aatggggacc ggcgcatttc taccacagac cggtcttcag gcttcagtcg tgatcgggtc
     5521 gttcgattcg ctcgggtcgt tcgacgcgct cggcgcgttc ggcccgctcg tagcgctcgc
     5581 cgctgccttg gcccgagcgg tcttcgcgtt ctggactctc gctgcgtccc gagcggtcac
     5641 gcccggagtt tgccgagcgg ccatctccac cgcggtggtc ttcacggcca tcgggtgagt
     5701 catcgagccc actggatcct tcccggcgtg gacgttcctc gcggcgatcg cgaatttcga
     5761 ttcgttcggc gcggaccttg ccgtccttgt cgacgcggcc ggtaatacgg atcaggcggt
     5821 tgcgatcggc ctcatcgtcg cgcccaccga ccacgcgggt atcgtcgccc agttcgacgg
     5881 tgcgtccgcc ggccgacagg cgccggccgc tgcgctcggt aatacgcgcc tcgatcgaga
     5941 ccgcgcgggc gcgctcggtg tattcccggg ccgggctgcg ctcgactcga ctggcctgca
     6001 ggcgcccgtc gccgaaacgg ccacggacga ccacctcggc accttcgggc aattcgacgg
     6061 cttcgacccg caggccttcg atacggccat ctcgaacgcg gccgatcacg ctgtgttcga
     6121 tttcactgga ttcggcacgg tcgatgcgcg acgcaacgat ggcgccgtcg gcatcgcgga
     6181 ggcccgagac ccgtaccgtg tcgccgatcg ccagtgcgtc ggcggcgtcg gaacggacga
     6241 cccgctgccc catcacttcg acgttcttgc cgtcgacgcg cgtcaccggg ccgactaccg
     6301 cgtggacaat cgcgactcgc ttcgcttcga ggccgcgggc gctcggcgtg gcctcgatcg
     6361 ccacgacctg accgatggcc aggctgtgag tcgatgagcc ggcgccgttg gcggtgaccg
     6421 gcgtcgaatc ctcatagtgc acttcgaggc cattgacgca gatcgatgca aatccggtaa
     6481 ttgtgccgac caccgcgccg gtttcgccag ccgcgatcgg cagaacgact tggccagtgc
     6541 cgccgattcc gcccagtgcc agttcagcgg ggctgccggt gccgccgatc ccgccgcgcg
     6601 ctggcgcgct ggtgccgcca atgccgccgg ccggtccttc ggcgcaggct gcagcggcaa
     6661 ggaccggtgt cgcgctcagt gctgttgccg ccgcgaggaa aaaggggaga atatgacgca
     6721 tgcgagacat cttaacgtct ctccccgtcg ttgtggtcac ggcgctcggc gtcgtcggcg
     6781 taaaagtaaa cgcccagggt cagccgcttg cgcggcccgt cgccggccgc cgcgcgcgct
     6841 tcggagtcgg cggccttgcg gttgatgttg cgcagcaggc gcatggtgcc gttctcggca
     6901 tccttgcgca gctgggcgag gtcgtcggca tgtacttcgt cgtagtggac acagcgttcc
     6961 agccaggggc tggcgccctc ctcgatttcc aggttgtcgg cgatggcggc agcgtggtcg
     7021 tgcaggttca ggccgaggta gaaggccttt tcctcaaagc cacgctgcgg aacgaaggcg
     7081 ccggccttga ggcagacgcg atcctcgtcg tcgagtacga cgacaccgag gcgcagccat
     7141 tcgtcgagta ccgcgcgggc gcggatatcc ttactgacgc tggcaacgag gctttcgaac
     7201 gaaacagatt ggctttggct agacaggcgt ggcagtggca acgggttgcc ctcttcgtcg
     7261 gcaaagggcg gctcgcccca ggccgccgcc aggcgcatgc cgagcgagat ggtctcaggc
     7321 attggtgcct cgggaaggat ttctgcccgc aggcgcttga cgtccttgcg atggatgccg
     7381 gtgagcaggc tgatccggct gtcggtctgc gccttgcccg ggaccgggaa gtcacgatcg
     7441 gcgacttcga cgaacaggcc cttgagcaac tcggtcagtt gcagataggt gacgcccttg
     7501 ctcagcatca gccgcaccag aggccgaagg gcccggcgca gtgccttgag cagcaaatca
     7561 ggtgtcgcgt tcgcagttgg actggtcatg ttctcgcctt ccttggaacc cactatacac
     7621 aagcgtggga aaattttcca caaaaatctt gacgtggaaa aatttcccac ctactatggg
     7681 ttcaacgcag cccgaatccg cactcaggga tgcgccagaa aaccggtggg gcgactaatc
     7741 agaaagaagg gcgcactgcc agatcaggac caaccatgaa ggagaagcaa atgaaaacca
     7801 aacagatgat tcccgccctg ctcgctacga tgttcctcgc cgtcggcgcc gttcaggcgc
     7861 aatcgacttc cgacgacatc gtcggaggcg tcaagctacg cggcgacggt acggtagaca
     7921 acacgcagcc aggggctgtc ggcggcggca acgacatcgt cgatggcgtg aagcgccgcg
     7981 gtgatgggtc ggtggatgat tcgcagcctg gtgccccgtc gggcaacgac gacatcgtca
     8041 atggtgtgaa gcgccggggt gacggttcgg tcgacgattc gcaaccggga atggcgtcgg
     8101 gcagcgacga catcgtcaat ggtgtgaagc gccggggtga cggcagcatt gacgacaaca
     8161 gcatccctgg cagcgagact ggcgacgatc gccgttcgga tcgcgccaag gaggttctgt
     8221 cccgtgacgc gaaggagcgt cgctccgagc gcgccaagag ccgcatcgag gacaatcgcg
     8281 agcgccacga aacgcgggtg aagagtcgtg aagtggtcga cggcgttcga gttcgcaccg
     8341 agacgcggga gcggaccgaa gttcgggatc gcggcgagcg ggctgaacgt gctgagcgca
     8401 ccgagcgtgt cgaacgtgcc gagcgtgaag agcgcagcgg tcgcgactga tctcagcagt
     8461 ctagatccgt agcactgacg gagcgccgca ttgacggcgc tttgtcgtcc cggaggagtc
     8521 atggccaaaa acgaagccac cgcagcaacg aaacccagac gccggaccga tggccggtcg
     8581 cagcgtttcg acctggtgcg gtggttctcg tgggtggcgc tcgttgccgg cactgccgtc
     8641 acgctatcgg ccgcgttctc gctgtcggcc tttctcacca gccgcatggt cgcgcaggaa
     8701 gcgagcctta ctgcggactt cgtccgcagc gtcatcgctg cagatgacgc gtatcgagtc
     8761 ttcgcacagc ccgaaagcgc caccgccgaa gaccgtattc gcgccgtcga cgtcttcaca
     8821 catctggccg aaatgcccga tgtaatccgt accaacgtat acgccgccga cggcaccgtg
     8881 ttgtggtcaa gcgacgccga gatgatcggc cggcggtttg ccgaaaacga cgaactgaag
     8941 gaggcgctgc gtgccgaggt cgtcgtccac agcggcgtgg tcgatcgcga gacgctgacc
     9001 aagcccgagc accgcaatct cgaccttcgg catgagcggt ttgttgaaag ctacacgccg
     9061 atctttgcca agaactccag ggaggtgatc ggggtagtcg agctataccg gattcccgag
     9121 cagctgttcg gtgccattca tgatggccaa gtactgatct ggtccggcgc ggtcataacg
     9181 gcgatcttcc tgttcgtcgc cttttcggga atcgtcgcca aggcccagca ggtgatcgag
     9241 cgccagcacg aacagctgat cgagcaggaa ggactcgccg tggtcggcga aatgggttcc
     9301 gcggtagccc atggcttgcg caatccgctc gcgtcgatcc gctcgtcagc cgaagtggca
     9361 ctcggctcgg atattcccgc ggacgcacgc gagtgcgcca acgacatcat ttctcaagtc
     9421 gaccgtctgg agggctggat acggcgcctg ctcacctatg caaagccgag cacggcggca
     9481 ctcggcccgg tgacgatcga ccgggtcgtc aacgaatgcg ttggcgacta ccggcgggag
     9541 ctcgaaaagc gcgacatcca gcttctcgtc ggtgttgatg ccggcctgcc gccggtgcgc
     9601 ggcgaggcag gcatcctcgg ccagctgatc aacagcctga tcgtcaacgc ggccgaggcg
     9661 atccaaggcc ccggaaagat ccaggtgagc gcaaggcgtc atgccgaagg cgttatggtc
     9721 gaagtgctcg acgacggccc cggtctgaac gacccggaca tcgagaaagt ctttaccccg
     9781 ttctacacca ccaaacaaaa gggccttggt ctcggcctgc cgctggtcaa gcgcgtcatc
     9841 gagcgttttg gtggcacggt cgccatcacc aatacgcaac cgcgcggtgt cagcgttcgg
     9901 ttgttcctgc aaacacaccc gcaagaggtg caatcatgag tgacgcaatt ctgatcgtcg
     9961 aggacgaagc gatcctcgcc aaaaacatcc gcatattctt cgaacgcgcc ggctacgatg
    10021 ctcgcgtcgc gcagagcgcc gaagatgcgc tgatggaact ggacgacttc cgtccggacc
    10081 tcgtgctcct cgatttccag ctgccgggca tcaacgggtt ggaaatgctg gaattgctgc
    10141 gcgagcgtga gccacagctg cccgtggtca tgctcaccgg tcaaggcacc gtcgaacttg
    10201 cagttcgcgc gatgcgcagc ggcgcggtcg attttctgag taaaccagtt gccctccagc
    10261 atctgcgcgt ggtcgtcgac cgcctgctgg acgagcgccg taacgatacc gccagccagt
    10321 attaccgggc gcgcagcgca accggagttg ccgcgttgat cggagaatcg cagcccatgc
    10381 gccagctcaa ggaaacaata cggacgatcc tcgaggcgga agcgacgatg accgatgctg
    10441 cgccgccaac gattctggtg accggcgaaa ccggaaccgg caaggagctg gtcgcgcgcg
    10501 cactgcactg cgacggacca cgcagtgacg ggccattcat cgagctcaac tgcgccacgg
    10561 tgcccgcgcc gctgctcgag gccgagctgt tcggctacga gcgcggcgcc ttcaccgatg
    10621 ccaaggagcg gaagattggc ttggtcgagg cggcgacggg cggcaccctg ttcctcgatg
    10681 aaatcggcga catggatccg gccctgcaag caaaagtgct caagctgctc gaagagaagg
    10741 ttgtccgccg gctcggcgcc atccgcgacc agaaggtcga cgtccgcatc atcgccgcga
    10801 cccatcagcc gctcgaccag ctggtggcgc agggcaagtt ccgggcagac ctctacttcc
    10861 gcctgcgcat ggtgcagatc gaactgccgc ccctgcgcga gcgcggcgac gacatcctgc
    10921 tgctggccaa ggaattcctc gcgcagcagt cgctccgata ccgcaagcag gggctgtcat
    10981 tcagtcaaga cgcgcagacc gcgttgcgca atcacgcctg gatcggcaac gtccgcgaac
    11041 tgcgcaacct gatcgagcag accgtcctgc tcgcaagcga tccgatcgtc gccgccaagc
    11101 agcttgcctt ctacctgccg tcgaaggtcg tgcccaccga ccttgagcag cccgagaaag
    11161 gccggcgcgc cagtgatctc gttgtcggcg acgaaatccg cccgctgtcg gaagtggagc
    11221 gggggctgct ggtcgaggcc ctgcacaaga cgtcctggaa cgtcacgcgc gccgcacgtc
    11281 ttcttggcat ctcgcgcgat acgcttcgct accggatcga gaagcacaac ctctcgcgct
    11341 actcctgagc gtcagcgcag cagccaggga gtcgcggcga gcagcgcgat gacgatgatc
    11401 agaatcgcca gcaggcggtt ctgcacctgc tgtgccgcgg cgattcgttc cagcgtttcg
    11461 cgctgagccg aatcgggtgg gcgagacaag gcctggtgcg cgagccgcgg cagttgcggc
    11521 agcgatttgg cccagttcgg cgattcgttt cgcaaggccg cgatgagcgc acgccagccc
    11581 acctgttcgc tcatccactg ctcgagatag ggcttggcgg tcttccacag gtcgagctcg
    11641 ggatcaagct ggcgcccgag accttcgatg ttgagcagcg tcttctgcag aaggaccagt
    11701 tgcggctgga cctcgacgtt gaagcgacgc gaagtctgaa acagccgcaa caaaacccgg
    11761 ccaaacgaaa tctcgcgcag cggccgatca aagatcggtt cgcagaccgc gcggatcgcc
    11821 gcttcgaact cgtcgggtcg tgtctccctt ggcgcccagc cagcctcgat gtgcgcgagg
    11881 gcgacgcgct tgtagtcgcg cttgaagaag gccaggaagt tctgcgccag gtaattcttg
    11941 tcgacgtcgg tcagcgttcc catgattccg aagtcgagcg cgatgtattg cccttcgggc
    12001 gaaacaaaga tgttgcccgg atgcatgtca gcgtgaaaga agccgtcgcg gaacacctgc
    12061 gtgaagaaga tctcgacgcc ggcgctgccc agacggtgca ggtcgatgcc ttggctgcgc
    12121 agacggtcga tctggctgat cggtgtgccg tgcatgcgct ccatcgtcat caccgatgac
    12181 gagcaccagt cccagtagac ctcgggcacc cgcagcagcg tcgactcggc gaagttgcgt
    12241 cgcagctgcg agcagtttgc cgactcgcgc atgaggtcaa gctcatcgtg caggtgcttg
    12301 gcgaactccg cgacgacctc gcgcggtttc aagcgcttgc catcggacca cagcgtttcg
    12361 gcgagccagg caaaggtgtc gagcagcgcg aggtcgttct cgatggcggc aagcattccc
    12421 gggcgcagca ccttgactgc gacttcccg
//
LOCUS       DATDAA010000030         7885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_456567,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000030 DATDAA010000000
VERSION     DATDAA010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7885
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456567"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VIS73_01090"
     CDS             <1..307
                     /locus_tag="VIS73_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY5761781.1"
                     /translation="HPQHALASRQQKSGGAILGFEVQGGRSEAWRVVDHCRLLSITGN
                     LGDVKTTLTHPATTTHGRISAEARAAAGISESLLRVAVGLESLSDLQDDLERGLRLV"
     gene            complement(304..2172)
                     /locus_tag="VIS73_01095"
     CDS             complement(304..2172)
                     /locus_tag="VIS73_01095"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY5761782.1"
                     /translation="MTIAHRAVASFLVAISLSITSEVAPAAPRKVALVVGNSAYAEAP
                     LRNPANDARAMARTLRELGFEVIERVDATQKDMNRAVTAFGEKLGADSIALFFYAGHG
                     LQVRGKNFLIPVDARITSEASVRSESVDVDIVLEQIASTGSGLNFVILDACRNNPFER
                     RFRATGGGLAQMDAPKGTFIAYATAPGRTASDGDGDNGLYTQELLKAITAPGLKVEDV
                     FKRVRVEVARASNDNQLPWESSSLTGDFYFKPPQAVATKASGPNADALELELWKSAQQ
                     LDAIEAYRAYLDAYPDGRYARLAGIGIKQAERRSRERTKVDAAMQAALAEANARARAA
                     EEAARTAAALARLGAAGGRAAGSSETEAALRAEIEQANARADAAEQAAKAARLGVTEV
                     LASPVAHASPIVDASGSGESVLAQEPGQPREGDEWTYRFIDRWKRQPVADYRIRLESV
                     GADAIKERIDYIDPKAAKPGAEILRARWIDRNSVTSQLDAIPLTAPAHSEFTITEWLP
                     YQPLPASLKKGHVWEGPFTIDGEPCSGSAELGKEESIDVPAGTFKARLIELECQRDAP
                     LVGRSPGDTTRLIRLRIWHANGLYRPLRIEKSIKGNFQEFDRDSIELVRFSRAAFP"
     gene            2274..3413
                     /locus_tag="VIS73_01100"
     CDS             2274..3413
                     /locus_tag="VIS73_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HEY5761783.1"
                     /translation="MTAADNDFDIPPRRDYLRSMRWQKYAGRDVIPLWVADMDFAAPP
                     PVIAAVRAVADDPVYGYAATPDGTAEAVAAYCAQHYGWAVDPSTLLWLPGLVSGLHVA
                     VRTVAGAGGVLVMPPIYPPFLSAPGLQGAQAVTVPLLVDAAGYRFDIDGIDAALRRDP
                     KTRLLMLCHPHNPVGRAWRRDELAALAALAEQHDLVVCSDEVHCDLLLEPGARHIPFA
                     TLPGMAERTITLMAPSKTYNVAGLGAAWAVVGDPALRTRVRTAMNGIVPYIDAFGFAA
                     LNACVGGACEDWRQGLLAYLRQNRDRVAAFAGTAGLAMPLPEASFLAWFDTRHIPDAH
                     RRIEAAGVGLSNGADFGAPGFLRLNFGTQRRLLDEALSRMVGVLG"
     gene            complement(3415..4143)
                     /locus_tag="VIS73_01105"
     CDS             complement(3415..4143)
                     /locus_tag="VIS73_01105"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY5761784.1"
                     /translation="MILFAADIHLSPSVPGIVERFLGFLRGPAIDAESLYLLGDVFEY
                     WAGDDDLDDPFNRRIVAALRELADRGTRLFFVAGNRDFLIGDGFAQAAGITLLADPQV
                     LSVPSWQFVLSHGDRFCTQDTAYMAFRETVRAPAWQAQFLAQPLALRKQQIAAIRSES
                     ERTKSAKANSAPALMDVDPGTVDDFLRDYGYATLIHGHTHRPATHDHLVDGIHCQRWV
                     LSDWHDDRAEIIVWDGETLSRRAV"
     gene            complement(4189..4683)
                     /locus_tag="VIS73_01110"
     CDS             complement(4189..4683)
                     /locus_tag="VIS73_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5761785.1"
                     /translation="MMIKLHTSLGTIEIELDAEKAPATVANFEQYVRDGHYDNTIFHR
                     VIDGFMIQCGGFEPGMNQKPTRKPVDNEAANGLKNKRGTVAMARTNDPHSATAQFFIN
                     IADNDFLDFKSPSGSGWGYCVFGKVSGGMDVVDKIRAVRTVTRGFHQDVPAEDVLITS
                     AEIV"
     gene            complement(4697..5251)
                     /locus_tag="VIS73_01115"
     CDS             complement(4697..5251)
                     /locus_tag="VIS73_01115"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5761786.1"
                     /translation="MKLLIAIAALIPSLALAVNPQVALKTSEGTIVVELAADKAPGST
                     QNFLQYVQDKHYDGTIFHRVIPNFMIQGGGFTADMKQKATRAPVGNEAKNGLRNARGT
                     LAMARTGDPHSATAQFFINHKDNTFLDYPGQDGWGYAVFGKVVSGMEVVDKIAHVSTG
                     RVGPFSDVPQQPVLIESATLISKH"
     gene            complement(5290..6489)
                     /locus_tag="VIS73_01120"
     CDS             complement(5290..6489)
                     /locus_tag="VIS73_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY5761787.1"
                     /translation="MTRRRLRPLALVAALGVALAVPAAGDRDSELRFADAGPEPLLAQ
                     VLHEVSRQRMDTALAAAEKLTAEYPTFRLGHLIVGDLLLARSRPLATFGNATGQDGRV
                     AALREEARMRLAAYTEKPAAEQLPRQILQLSTNQRHAVLVDTSRSRLYLFANDAPVPR
                     LVADYYVSQGRAGADKWREGDLKTPLGVYHVTSFLPPDSLTDFYGSGAFPINYPTHRD
                     RRQGRTGHGIWLHGTPPDAYSRPPRASEGCVVLANPDFEALSASIDSGTTPVVIANGI
                     DWLPAKIWQAERNSLREAIEIWRLDWESRDTDRYLSHYAADFRSDDQSRAAFAQSKRL
                     INGGKAWIKVSLSNLNLLANPGEDDFVVANFEQDYRSNNLNNTMRKAQHWSRRNGRWQ
                     IIYEGSA"
     gene            complement(6516..7625)
                     /locus_tag="VIS73_01125"
     CDS             complement(6516..7625)
                     /locus_tag="VIS73_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5761788.1"
                     /translation="MPHRPAITLRKLLFASFLGACACLVQAQETGKELAEANRLFKQG
                     KHAAALSSVDGHLTQNPTDAQGRFLKGLILTEQNRLEDATAVFVSLTNDFPELPEPYN
                     NLAVLYAQQRHYDKARNALEMAIRTHPAYAIAYENLGDVYAKLASQAYGKALQIDSGN
                     AVAQDKLSLIRELFGTTAKPPKAAATAPRVAAAQPAVVDARASAAAPAPVKLTVAEKP
                     SSITKTSPPSSNSAATSTAKDPAVATPPSSTAAADSAGETLQAVTQTLEAWALAWSSK
                     DFSSYLSHYEPDFSPNTSTSHKDWAVSRALRVGKPGDIDVAISDVRVALDDEGRAVAR
                     FHQRYQSDNLKSNTYKTMVLKRIGNRWLIVEERIG"
BASE COUNT         1301 a         2377 c         2657 g         1550 t
ORIGIN      
        1 ccatccgcag cacgcgctgg cgagccggca gcagaagtcg ggcggcgcga ttctcgggtt
       61 tgaagtgcag ggagggagaa gcgaagcctg gcgcgtcgtc gaccattgcc gcctgctgtc
      121 gatcaccggc aatctcggcg acgtcaagac cacgctgacg cacccggcaa cgacgacgca
      181 cggccgcatc agtgccgagg cgcgcgccgc ggctggaata tcggagagcc tcttgcgtgt
      241 cgcagttggc ctggagtccc tgtccgattt gcaggatgac ctcgagcgcg gcttgcgcct
      301 cgtctagggg aatgccgctc ggctgaatcg gaccagctcg atgctgtccc ggtcgaactc
      361 ctggaaattg cccttgatgg atttttcgat ccgcagtggc cggtagagcc cgttagcgtg
      421 ccagatgcgc agccggatca accgggtggt gtcgccaggg gatcttccga cgagcggggc
      481 gtcgcgctgg cactccagct caattaaacg tgccttgaac gttccggccg gaacgtcgat
      541 gctctcctcc ttgccgagtt cagccgagcc actgcagggt tccccgtcga ttgtgaacgg
      601 gccttcccag acatggcctt tcttcaagga agcgggcaag ggctgatagg gcagccactc
      661 ggtgatcgtg aactcgctgt gcgctggggc ggtcagcggg atcgcatcga gctgggaggt
      721 aactgagttc cggtcgatcc agcgcgcgcg caggatttcg gcgccgggct tggctgcctt
      781 tgggtcgatg tagtcgatcc gttccttgat cgcgtcggcg ccgaccgatt caagccggat
      841 tcggtagtcg gcaactggtt ggcgcttcca gcggtcgatg aatcgatagg tccattcgtc
      901 accctcgcgc ggctggccag gttcctgagc caacacggac tcgcccgacc cggaagcatc
      961 cactatcggt gacgcatgcg cgaccggcga tgccaggact tcggtgacgc ctagtcttgc
     1021 agcctttgcg gcctgttcgg ctgcgtcggc gcgtgcattg gcctgttcga tttccgcgcg
     1081 caatgccgct tccgtctcac tgctgcccgc tgctcggccg ccagcggccc cgaggcgtgc
     1141 cagcgccgcg gcggtacggg ctgcttcctc cgctgccctg gctcgtgcgt tggcttcggc
     1201 aagcgcggcc tgcatcgccg catcgacctt cgtgcgctca cggctgcggc gttcggcctg
     1261 tttgatcccg atgcctgcca gcctcgcgta gcggccgtcg ggataagcgt cgaggtaggc
     1321 tcggtacgct tctatggcat cgagttgctg tgccgatttc cacagctcga gctcgagcgc
     1381 gtcggcgttt ggcccactgg ccttggtcgc gacggcttgg ggcggcttga aatagaagtc
     1441 gccggtgagc gacgacgact cccatggcag ctggttgtcg ttggacgccc gcgcgacttc
     1501 gacacgcacg cgcttgaata cgtcctcgac cttgagccct ggcgcggtga ttgccttgag
     1561 caactcctga gtgtacagcc cgttgtcgcc gtccccgtcg cttgcggtcc gtcccggcgc
     1621 cgtcgcgtag gcgataaacg ttcccttggg cgcgtccatt tgcgccaggc cgccaccggt
     1681 cgcgcggaag cgacgctcaa agggattgtt gcggcaggca tcgagaatga caaagttaag
     1741 gccagagccg gtgctggcga tctgctcaag aacgatatca acgtcgacgc tttccgagcg
     1801 gaccgaggct tccgaggtaa tgcgtgcgtc aacggggatc aggaaattct tgccgcggac
     1861 ctgcaggccg tgcccagcgt agaaaaaaag tgcgatcgaa tccgcaccga gcttctcgcc
     1921 gaacgcggtc actgcgcggt tcatgtcctt ttgcgtcgca tcgacgcgtt cgatgacttc
     1981 gaagcccagc tcgcgcagcg tgcgggccat ggcccgggca tcgttggccg gattccgaag
     2041 tggtgcttcc gcgtaggcgc tgttgcctac caccagtgcc accttgcgtg gcgccgcggg
     2101 cgcgacctcc gacgttatcg acagggaaat ggcgacgaga aacgaggcga cggcgcggtg
     2161 cgcgatggtc atcacaattg gcccaaagtg ctggaacagc cttagagtac tgataacgtc
     2221 aacacggcgc tacacatcag tgccgcgtca cggccaattg gcgagaaacg aacatgactg
     2281 ctgccgataa cgacttcgac attccgccac gacgcgacta cctccgcagc atgcgctggc
     2341 agaagtacgc gggccgcgac gtgattccgc tgtgggtagc cgacatggac ttcgcggcgc
     2401 caccgccggt gatcgccgcc gtacgcgcgg tcgctgatga tccggtctat gggtatgcag
     2461 cgacccccga tgggaccgcc gaggccgttg ccgcgtactg cgcgcagcac tatggctggg
     2521 cggtcgatcc atcgacgcta ctctggctgc cgggtctggt ctcgggtcta cacgtcgccg
     2581 ttcggacagt tgcaggggcc ggtggcgtgc tcgtcatgcc gccgatctat cccccgtttc
     2641 tgtcggcgcc gggcctgcag ggcgcgcagg cggtcacagt gccattgttg gttgacgctg
     2701 ccggttaccg cttcgatatc gacggcatcg acgccgcttt gcggcgcgac ccgaagacac
     2761 gcctgctgat gctctgccat ccccacaacc cggtcggacg cgcgtggcgg cgtgacgagc
     2821 tggcggcgct ggcagcgctc gcggaacaac acgatctggt ggtctgcagc gacgaggtgc
     2881 attgcgacct gctgctcgaa ccgggtgcca ggcatattcc cttcgcgacg ctgccaggaa
     2941 tggccgaacg aacgatcacg ctgatggcgc cgagcaagac ctacaacgtc gccggcctcg
     3001 gtgcggcctg ggcggtcgtc ggcgatccgg cgctgcgcac gcgggttcgc acggcgatga
     3061 acggtatcgt tccttatatc gacgcctttg gttttgccgc gctgaatgcc tgcgtcggtg
     3121 gcgcctgcga ggactggcgg caggggctgc ttgcctacct gcgccagaat cgcgaccggg
     3181 tcgcggcctt tgccggaaca gccgggctgg cgatgccgct accggaggcg agctttctgg
     3241 cgtggtttga cacgcggcat attcccgacg cgcatcggcg gatcgaggcg gccggggtcg
     3301 ggctgtcgaa tggcgcggac ttcggcgcgc ccggatttct ccggctcaat ttcggcacgc
     3361 agcggcgact gctggacgag gcactgtcgc ggatggtcgg cgtactgggc tgattcagac
     3421 ggcgcggcgg gacagcgtct caccatccca gacgatgatt tcggctcggt cgtcgtgcca
     3481 atcggaaaga acccagcgct ggcagtgaat gccgtcgacg aggtgatcgt gggtcgccgg
     3541 ccggtgcgtg tgaccgtgga ttagcgtcgc gtagccgtaa tcgcgtagga agtcatcgac
     3601 ggtgccggga tcgacgtcca tcaacgcggg cgccgagttg gccttcgccg atttcgttcg
     3661 ttcgctttcg ctgcggattg cggcgatctg ctgcttacgc agggccagcg gctgggcgag
     3721 gaactgcgcc tgccatgctg gcgcccgcac ggtttcgcgg aacgccatgt aggccgtgtc
     3781 ctgcgtgcag aatcgatcac cgtggctgag aacgaactgc caggacggca ccgaaagtac
     3841 ctgcgggtcg gcgagcagcg tgatgcccgc ggcttgtgca aagccgtcgc cgatcaggaa
     3901 gtcgcggttg ccggcgacga agaagaggcg cgttccgcgg tcggccagtt cgcgcaatgc
     3961 ggcgacgatc cggcgattga agggatcgtc gagatcatcg tcgccggccc agtactcaaa
     4021 gacgtcgcca agcaggtaga gcgattcggc gtcgatggcg gggccgcgca gaaagcctag
     4081 aaaccgttcg acgattccag ggacagacgg cgacaggtgg atgtcggcag cgaagaggat
     4141 cacgggaatt cgcgtgccgt cagccggccc gcgatcagga aagcggcgct agacgatttc
     4201 ggcgctggtg atcagtacgt cctcggccgg cacgtcctga tgaaagccgc gcgtcacggt
     4261 gcggacagcg cggatcttgt cgaccacgtc catgcccccg ctgaccttgc cgaagacgca
     4321 atagccccag ccgctgcccg acggcgactt gaaatcgagg aagtcgttgt cagcgatatt
     4381 gatgaagaac tgcgcggtcg ccgaatgcgg gtcgttggtc cgcgccatcg ccaccgtgcc
     4441 gcgcttgttc ttgagaccgt tggcggcctc gttgtcgacc ggcttgcgcg tcggtttctg
     4501 attcatgccc ggctcgaacc cgccgcactg gatcatgaat ccatcgatga cgcgatggaa
     4561 gatcgtgttg tcgtaatgcc cgtcgcggac gtactgctcg aagttggcaa cggttgccgg
     4621 tgccttttcg gcgtccagtt cgatttcgat ggtgccgagg ctggtatgca gcttgatcat
     4681 gatgcgggcc tttcgctcag tgcttggaga tgagggttgc cgattcgatg agtaccggct
     4741 gttgcggaac atccgagaac gggccgaccc ggccggtgct tacgtgggcg atcttgtcga
     4801 cgacttccat tcccgaaacg accttgccga acacggcgta gccccagcca tcctggcccg
     4861 ggtagtcgag gaaggtgttg tccttgtggt taatgaagaa ctgcgcggtg gccgaatgcg
     4921 ggtcgccggt ccgcgccatc gcgagcgtgc cgcgggcatt ccgcagtccg tttttcgcct
     4981 cgttgccgac cggcgcgcgc gttgccttct gcttcatgtc ggcggtgaag ccgccgccct
     5041 ggatcatgaa gttggggatg acgcggtgga agatggtgcc gtcgtaatgc ttgtcttgca
     5101 cgtactgcag gaagttctgt gtgctgcccg gagccttgtc tgccgcgagt tcgacgacga
     5161 tcgtgccttc gctggttttc agcgcgactt gcgggttaac ggccaaagcc aggctgggga
     5221 tcagcgcggc gattgcaatc agcagtttca tggagactcc aagttcagtg ggaaggatgg
     5281 aaaaacggat caggcgctgc cttcgtagat gatctgccaa cggccgttgc gccgagacca
     5341 gtgctgtgcc ttgcgcatcg tgttgttgag gttgttgctg cggtaatcct gttcgaagtt
     5401 cgcgaccacg aagtcgtcct cgccgggatt ggcgagcagg tttagattcg acagcgatac
     5461 ctttatccag gctttgccgc cattgatcag ccgtttactt tgcgcgaatg cagcacggct
     5521 ctggtcgtcg ctgcggaaat ccgctgcgta atggctcagg tagcgatcgg tatcgcggga
     5581 ctcccagtca aggcgccaga tctcgattgc ctcgcgcagg ctgttccgct cagcctgcca
     5641 gatcttggcc ggcagccagt cgatgccgtt ggcgatgacc accggcgtgg tcccgctgtc
     5701 gatcgatgcc gaaagagcct cgaagtccgg gttcgcaagt accacgcagc cttcggaggc
     5761 gcgcgggggt cgcgagtagg cgtctggtgg cgttccgtgt agccagatcc cgtgcccggt
     5821 ccggccctgg cggcgatcgc gatgggtggg ataattgatc gggaaggcgc cgctgccgta
     5881 gaagtcggtc aggctgtcgg gcggcaggaa actcgtgacg tgatagacgc caagcggtgt
     5941 cttcaggtct ccctcacgcc atttgtcggc cccagcgcga ccctgcgaga cgtaatagtc
     6001 ggccaccagt cgcggcaccg gagcgtcgtt ggcgaacagg taaagccgcg accggctggt
     6061 atcgaccagt accgcgtggc gctggttcgt cgacaactga agaatctgcc gcggcaactg
     6121 ctcggcggca ggcttttcgg tgtacgccgc gaggcgcatc cttgcttcct cgcgcagcgc
     6181 cgcgactcgc ccatcctggc cggtcgcatt gccgaaggtt gcgagcggac ggctgcgcgc
     6241 gaggagcagg tcgccgacga tcaggtgtcc gaggcggaac gttgggtatt cggcggtcag
     6301 cttttcggcg gcggcgagcg ccgtatccat gcgttgccgg ctgacttcgt ggagaacttg
     6361 ggcgagcagc ggctcgggcc cggcatcggc gaagcgcaat tccgagtcgc gatcgccggc
     6421 ggcgggaacc gcaagggcaa ccccgagcgc tgcgaccagt gcgagcgggc gaaggcgtcg
     6481 tcgagtcacg ctttgcgtcg ccgggcggtt agacatcagc cgatccgctc ttcgacgatt
     6541 agccaacgat tgccgatacg ctttaggacc atcgtcttgt aggtgttcga cttgagattg
     6601 tccgactggt agcgctggtg aaagcgggct accgcccgac cttcatcatc aagcgcgacg
     6661 cggacgtcgc tgatcgccac atcaatgtct ccgggcttgc cgacgcgtag ggcgcgcgat
     6721 accgcccagt ccttgtggct ggtcgatgta ttgggggaga agtcgggttc gtagtgcgac
     6781 aggtagctgc tgaaatcctt gctcgaccag gcaagcgccc aagcttcgag tgtttgcgtc
     6841 accgcctgca gcgtctcgcc ggccgagtcc gctgccgcgg tgctgctcgg cggcgtggcg
     6901 acggccgggt ccttggccgt agaagtggcg gcagagttgg acgagggcgg gctcgtcttg
     6961 gtgatcgacg aaggcttttc agcaacggta agtttgaccg gagcgggcgc tgcagcgctt
     7021 gcacgcgcat cgacaacggc cggctgtgca gcggcgacac gcggcgcagt cgccgctgcc
     7081 ttggggggct tcgccgtggt tccgaacaat tcgcggatta gcgacagctt gtcctgtgcc
     7141 acggcattgc cggaatcaat ctgcagcgct ttgccatagg cctggctggc cagtttggcg
     7201 tagacgtcgc cgaggttttc gtaggcgatt gcgtaggcgg gatgcgtgcg aatcgccatc
     7261 tccagcgcgt tgcgagcctt gtcgtaatgg cgctgctggg cgtagagcac agcgaggttg
     7321 ttgtagggct ccggcagttc cggaaagtcg ttggtcaggc tgacgaatac ggccgtggcg
     7381 tcttcgaggc ggttctgctc ggtcaggatc aggcccttga gaaagcgtcc ctgggcgtcg
     7441 gtcggattct gtgtcagatg cccgtcaacg ctgctcagcg ctgcggcgtg ctttccctgt
     7501 ttgaacagcc ggttggcctc agccagttcc ttgccggttt cctgagcctg aaccaggcat
     7561 gcgcaggcgc ccaggaagga tgcaaagagg agttttcgca gcgtgatcgc gggccggtgc
     7621 ggcacggacc gatcagcgtg cggctgccgc atgcggattg agaagtaggg aagcatggcg
     7681 taatatactg gcgcttagcc taaatggctt aacaaacaaa gaattatatc aacaggctac
     7741 agcggttccc aagcacgcga cggcgactcg cgggtgttgc tgaccaatcc gtcgaattct
     7801 gccgattcta gccgctggcc ttccatcccg acccaatcaa atgctcagaa tctacaacac
     7861 gctgtcacgt gagaaacagg agttc
//
LOCUS       DATDAA010000031         3413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_351826,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000031 DATDAA010000000
VERSION     DATDAA010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351826"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VIS73_01130"
     CDS             complement(<1..1241)
                     /locus_tag="VIS73_01130"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEY5761789.1"
                     /translation="MKVLILGSGVIGTTMAYYIARAGHQVTVVDRQPGPALETSHANA
                     GEVSPGYSAPWAGPGIPLKAIKWMLMHHAPLVIRPMLDPAMWRWGLAMLRNCTAARYR
                     INKSRMVRLAEYSRDCLKALRAETGISYEERMQGTLQLFRTQKQLDGVAKDTQILADY
                     GVDFQVLDREGYLRYEPALALVRDKFIGALRLPGDETGDCYLFTQKIAQMAADLGVEF
                     RFGTTVEGIETAGCQVSGIRTDTGTLIADRYVVALASYSPLLLKPIGIDLPVYPVKGF
                     SITVPITDTRMAPESTIMDETHKVAVTRLGERIRVGGTAQLSGYDLELNEGRRKTLEF
                     VVSDLFPKGGDVSRAEFWTGLRPMTPDGTPIVGRCRYPNLLLSTGHGTLGWTMAAGTG
                     RVMADLISDRAPEIDIDDLGVA"
     gene            complement(1287..1814)
                     /locus_tag="VIS73_01135"
     CDS             complement(1287..1814)
                     /locus_tag="VIS73_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEY5761790.1"
                     /translation="MKPLILPLLLLLAACQGVPDDGRPPVEAVPRVDLQRYAGVWHEI
                     ARLPMPFQKQCVGDTTAEYRLRDDGAVAVLNRCRTASGFDQAEGKATVVEGSQNARLK
                     VSFFWPFRADYWIIGLDADYRWAVVGDPSRKYLWVLARSPQLPPEQLEAALTIARAQG
                     YDLSPLIHSQPRPGN"
     gene            2083..2373
                     /gene="groES"
                     /locus_tag="VIS73_01140"
     CDS             2083..2373
                     /gene="groES"
                     /locus_tag="VIS73_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY5761791.1"
                     /translation="MKIRPLHDRVIIKRLEEERKTASGIVIPDSAAEKPDQGEVISVG
                     NGKVLDDGSVRKLEVKPGDRVLFGKYSGQTVKVDGEELLVMREEDIMGVVEK"
     gene            2414..>3413
                     /gene="groEL"
                     /locus_tag="VIS73_01145"
     CDS             2414..>3413
                     /gene="groEL"
                     /locus_tag="VIS73_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY5761792.1"
                     /translation="MAAKEVRFGDSARERMVAGVNILANAVKVTLGPKGRNVVLERSY
                     GAPTVTKDGVSVAKEIELKDKFENMGAQMLKEVASKTSDIAGDGTTTATVLAQSIVRE
                     GMKYVAAGMNPMDLKRGIDKAVVATVDELKKISKPCSTSKEIAQVGSISANSDDDIGQ
                     IIAKAMDKVGKEGVITVEDGKSLDNELEIVEGMQFDRGYLSPYFINNPDKQLALLDNP
                     YVLLFDKKISNIRDLLPVLEQVAKAGRPLLIIAEDVDGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLEDIAVLTGGVVIAEETGLTLEKATLKDLGQAKRVEIGKENTILI"
BASE COUNT          670 a         1052 c         1088 g          603 t
ORIGIN      
        1 gcgacgccga gatcgtcgat gtcgatctcg ggcgcgcggt cgctgatcag gtcggccatt
       61 acccgtccgg tgccggctgc catcgtccag cccagcgtgc cgtggccggt gctgagcagc
      121 aggttcggat agcggcagcg gccgacgatc ggcgtgccgt ccggcgtcat cggccgcagg
      181 ccggtccaga actcggcgcg cgagacatcg ccgcccttgg gaaacagatc actgacgacg
      241 aactccagcg tcttgcgccg gccctcgttg agttcgaggt cgtaacccga gagctgcgcc
      301 gtgccgccga cgcggatgcg ttcgccgagg cgcgtcaccg ccaccttgtg cgtctcgtcc
      361 atgatcgttg actcgggcgc catccgcgtg tcggtaatcg gcacggtgat cgagaagccc
      421 ttgaccgggt agaccggcag gtcgatgccg atcggcttaa gcagcagcgg cgagtagctg
      481 gccagcgcga ccacgtagcg gtcggcaatc agcgtgccgg tatcggtgcg gatgccgctc
      541 acctggcagc cagccgtttc gatgccctcg acggtcgtgc cgaagcggaa ttcgacgccg
      601 aggtcggcgg ccatctgcgc gatcttctgc gtaaacagat agcagtcgcc ggtttcgtcg
      661 ccaggcaggc gtagcgcgcc aatgaacttg tcgcgtacca gtgccagcgc cggttcatag
      721 cgcaggtagc cctcgcggtc gagcacctgg aagtcgacac cgtagtcggc gaggatctgc
      781 gtgtccttgg cgacgccgtc gagctgcttc tgcgtgcgaa agagctgcag cgtcccctgc
      841 attcgctctt cataggagat cccggtttca gcgcgcagtg ccttcaggca gtcgcggctg
      901 tactcggcga ggcggaccat gcggctcttg ttgatgcgat agcgggccgc cgtgcagttg
      961 cgcagcatgg ccagacccca gcgccacatc gcggggtcga gcatcggccg gattaccagg
     1021 ggcgcgtggt gcatcagcat ccacttgatt gccttgagtg gtatgccagg ccccgcccac
     1081 ggtgccgaat accccggtga tacctcgccg gcattggcat ggctggtttc cagcgccggc
     1141 cccggctggc ggtcgaccac cgtcacctga tgtccggcgc gggcgatgta gtaggccatc
     1201 gtcgtgccga tgacgccgct gccgagaatc aggaccttca tgccatgctc cctgcgggta
     1261 tggtccggct accgggcctt gtgccgtcag ttgccgggtc ttggttgcga atggattagc
     1321 ggcgacaaat cgtaaccctg cgcgcgggcg atcgtgagtg cggcctcgag ctgctcgggc
     1381 ggcaactgcg gcgagcgtgc aagaacccag agatatttcc gactcgggtc gccaaccacg
     1441 gcccagcggt agtcggcgtc gagaccgatg atccagtagt cggcgcgaaa cggccagaaa
     1501 aacgacacct tgagtcgcgc gttctggctg ccctcgacca ccgtcgcctt gccttcggcc
     1561 tgatcgaatc cgctggcggt ccggcagcga ttgaggactg ccaccgcgcc gtcatcgcgc
     1621 aaccggtatt cggcggtcgt atcgccgaca cactgcttct gaaagggcat cggcaagcgg
     1681 gcgatctcgt gccagacgcc ggcgtagcgc tgcagatcga cgcggggcac ggcctctacc
     1741 ggcggcctgc cgtcgtcggg gacgccctgg caggcggcaa gaaggagcag caacggcagg
     1801 atgagcggtt tcatggtcgt ctcaggagca tggaattgtt cgaagggcgt ccaccgattc
     1861 ggggagtcgt cgtagaccta gtgttcccat attaaggcgc ggccgacaat tgttttggcc
     1921 tgcgcacttg aaatgaccga ccgtcacccc tactattagc actcgttagc agcgagtgct
     1981 aatagaaaac cccgtttggt tcgtgtgcgt cggcaggtgc cggcagccga gagttagcaa
     2041 tcactcatct agcgacaaca tcgctcaata aggagaaagt tcatgaaaat ccgtcccttg
     2101 catgatcgcg tgatcatcaa gcgcctcgag gaagagcgga agaccgcgtc cggcatcgtc
     2161 attcccgatt ccgcggccga aaagcccgat cagggcgaag tcatttccgt cggcaacggc
     2221 aaggtcctcg acgacggttc ggttcgcaaa ctcgaagtga agccgggcga ccgcgtgctg
     2281 ttcggcaagt actcgggcca gaccgtcaag gtcgacggcg aggaactgct ggtcatgcgc
     2341 gaagaagaca tcatgggcgt ggtcgagaag taattcggcg acaaccgatt gaattaacaa
     2401 ggagatttaa caaatggcag ctaaagaagt ccgcttcggc gattcggcac gcgagcgcat
     2461 ggtcgctggc gtcaatatcc tggccaacgc ggtcaaagtg acgctcggtc ccaagggacg
     2521 caacgtggtc ctcgagcgct cgtatggcgc cccgaccgtc accaaggacg gcgtctccgt
     2581 cgccaaggaa atcgagctga aggacaagtt cgagaacatg ggcgcgcaaa tgctcaagga
     2641 agtcgcctcg aagacctccg acatcgccgg tgacggcacc accaccgcca ccgtgctggc
     2701 ccagtcgatc gttcgcgaag gtatgaagta cgttgccgcc ggcatgaacc cgatggatct
     2761 gaagcgcggc atcgacaagg cggtcgtcgc cacggttgac gagctgaaga agatctccaa
     2821 gccctgctcg acctcgaaag agatcgcaca ggtcggttcg atctcggcca actccgatga
     2881 cgacatcggc cagatcatcg ccaaggcgat ggacaaggtt ggcaaggaag gcgtgatcac
     2941 cgtcgaagac ggcaagtcgc tcgacaacga actggaaatc gtcgaaggca tgcagttcga
     3001 ccgcggctac ctgtcgccct acttcatcaa caacccggac aagcagctcg cgctgctgga
     3061 caacccgtat gtcctgctct tcgacaagaa gatcagcaac atccgcgatc tgctgccggt
     3121 acttgagcag gttgccaagg ctggccgtcc gctgctgatc attgccgaag acgtcgatgg
     3181 cgaagcgctg gccaccctgg tggtcaacaa catccgtggc atcctcaaga ccgttgccgt
     3241 caaggctccg ggctttggtg atcgtcgcaa ggccatgctc gaagacatcg ccgtgctgac
     3301 cggcggtgtg gtcatcgccg aggagaccgg cctgacgctc gaaaaagcca cgctgaagga
     3361 tctgggccag gccaagcgcg tcgaaatcgg caaggaaaac accatcctca tcg
//
LOCUS       DATDAA010000032        27682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_282007,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000032 DATDAA010000000
VERSION     DATDAA010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 27682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27682
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282007"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VIS73_01150"
     CDS             complement(<1..496)
                     /locus_tag="VIS73_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761793.1"
                     /translation="MKTQRPSPIAARHLATLCLAALLPGTAPAADSIRETVSQGSDRE
                     STAVTVYNDDLALVKEKRKVTLPAGMSRLALRDVSAQMRPETALLRAASGAPITLVEQ
                     NFDFDLLTPAKLLEKYVGKEVTVVRTNAATGEERSETARVLSANNGVVLRFADRIESG
                     VPGRI"
     gene            complement(634..2895)
                     /gene="clpA"
                     /locus_tag="VIS73_01155"
     CDS             complement(634..2895)
                     /gene="clpA"
                     /locus_tag="VIS73_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEY5761794.1"
                     /translation="MIAQELEVSLHMAFVEARQKRHEFITVEHLLLALLDNPSAAEAL
                     RACGGNVETLRKDLTQFIGQHTPTVSGDNDIETQPTLGFQRVIQRAILHVQSSGKKEV
                     NGANVLVAIFGEKDSHAVYYLHKLGITRLDVVNFISHGISKVQQPKAAPEHEEEKESA
                     EGNAAGPLESYTINLNALALQNKIDPLIGRDRELERVVQTLCRRRKNNPLLVGEAGVG
                     KTAIAEGLARRIVESEVPDILSEANVYSLDMGALLAGTKYRGDFEQRLKGVLKQLAGN
                     PNAILFIDEIHTLIGAGAASGGTLDASNLLKPALSQGQLKCIGATTYTEYRGIFEKDH
                     ALSRRFQKIDVVEPSVSETIHILKGLKPRFEAHHGIKYTAAALTAAAELSARYITDRH
                     LPDKAIDVIDEAGAAQRILPKSKQKKTIGKPEIEETVAKIARIPSQHVSSDDKSSLKN
                     LDRDLKAVVFGQDPAIDALAKAVKMARSGLGNPGKPIGSFLFSGPTGVGKTEVARQLA
                     FSLGIELIRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEEVTKKPYAVLLLDEIE
                     KAHPDIFNILLQVMDHGTLTDNNGRKADFRNVMIIMTTNAGQESLQKTTMGFTTEKKA
                     GDEMAEIKRLFTPEFRNRLDATISFRPLDHEVILRVVDKFLMQLEEQLHEKKVEAVFT
                     DALKEHLAKKGFDPLMGARPMTRVIQETIRSALADELLFGKLSHGGKITVDIDADGVV
                     KLSFADDKEAVTV"
     gene            complement(2896..3201)
                     /gene="clpS"
                     /locus_tag="VIS73_01160"
     CDS             complement(2896..3201)
                     /gene="clpS"
                     /locus_tag="VIS73_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY5761795.1"
                     /translation="MAAKTQKSVLEAERTRTALPPMFKVLLLNDDYTPMEFVVLVLQR
                     FFSMDREQATRIMLKVHNEGRGVCGVFPRDIAATKVELVLSFAREHQHPLACVMEES"
     gene            3552..3755
                     /locus_tag="VIS73_01165"
     CDS             3552..3755
                     /locus_tag="VIS73_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY5761796.1"
                     /translation="MAIGTVKWFNDAKGFGFITPDDGSEDVFAHFSAINMNGFKSLKE
                     GEKVSFEVTQGPKGKQATNIQRA"
     gene            complement(3833..4315)
                     /gene="moaC"
                     /locus_tag="VIS73_01170"
     CDS             complement(3833..4315)
                     /gene="moaC"
                     /locus_tag="VIS73_01170"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY5761797.1"
                     /translation="MTDPTLTHFDNAGRAHMVDVAEKAETRRLARAGGRIVMQAATLQ
                     RIVDGRADKGDVLGIARLAGIQAAKRTGDLIPLCHPIGLTHVAVDFEIDTAAHSVNCT
                     ATTECVGRTGVEMEALTAASVALLTIYDMCKAIDRGMVIESIGLIEKSGGKSGTWQRP
                     "
     gene            4422..5867
                     /locus_tag="VIS73_01175"
     CDS             4422..5867
                     /locus_tag="VIS73_01175"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY5761798.1"
                     /translation="MKPRFIAATAASCLLLALGAGFAGADNLPDLGDASALDVPPALE
                     RLVGEAQMNEIRLREPRFLDDAELTFYLQGLANRLLAAGDTAGVEIRLFALDDPSINA
                     FAMFGGYIGVNTGLITAAQSESEVAGVLAHEIAHVSQRHLARQVAAQKQVALGSMLAI
                     AVAIFAARSNAEVANAALATSQAAGIQAQLGFSRDHEREADRIGFEMMQRSGLDSRAM
                     VSFFQRLQRAGRVYENNAPTYLRTHPLTLDRITDLEHRVAQAAPYAQRNGGASEFPLI
                     QAKLLSMRGPTADALEGARDRYDKAADKPVSSYGLAVALTRTLQFDEAARVIADADAR
                     SPLVQRLAAEIAAARGQRDEAEQRYRQALAAHGERPALWYGLADLLLASGQHEQAREL
                     LDRRLRKVNDDPDAHARLARAYEGLGRIASRHRAQAEAYQLRGQTLAAIEQLELAQRA
                     GTSDFVEQSMIDARLRELKRRQSDRAPDARK"
     gene            5864..6106
                     /locus_tag="VIS73_01180"
     CDS             5864..6106
                     /locus_tag="VIS73_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY5761799.1"
                     /translation="MSEGQPTADREIDVRGLHCPYPLLAAKKALATMSAGEHLRVLAT
                     DPNAAKDFRLFAEASGNALVFAGGEAGALTFVFRRR"
     gene            6128..7894
                     /gene="pabB"
                     /locus_tag="VIS73_01185"
     CDS             6128..7894
                     /gene="pabB"
                     /locus_tag="VIS73_01185"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEY5761800.1"
                     /translation="MSAVSIEALEPFVLLETPETLRVFRRQLEAAPVSCADDLERLLG
                     PDTAYRVVAFDYELGHSIEPRSGDCASGAVGTVWRFAECDEIPLAECDAYIERHVAVL
                     PADARAAAVADLAIGLDEAAYVAGVARIQRWIADGDCYQVNYSFPLHLRTIGHPLALY
                     AALRRRQRGLWSACILTAERRILSCSPELFVAKRGDELACRPMKGTRPRSADAARDAG
                     NRAELDAAEKDRAENVMIVDLLRNDLGRVAVPGSVRVDRLFEVESYPTVHQLVSSIRA
                     KAPGRRMPEIVRALFPCGSITGAPKLRAMQIIGALETAPRGIYTGAIGCLMPDDDFTL
                     NVAIRTLDLEAGGRARLGVGSGIVADSVPADEYRECLLKGEFATGLPAPFSLIETLRL
                     ENGALPRLAGHLARLADSARRLGFRYPAYEISAALQARAAENPLGVFRLRLLLAPSGS
                     VSTTVQPFVPLGDDRRITLADGRLDSADWGLRFKTTRRARYDEALRRLPPEVFDTVFL
                     NERDELCEGARSNLFLLIEGRLLTPAVDCGLLPGVLRAELLASGRAAEAVLGLDDLRR
                     AEAIYAGNALRGLVSVTLATDS"
     gene            complement(7919..10750)
                     /locus_tag="VIS73_01190"
     CDS             complement(7919..10750)
                     /locus_tag="VIS73_01190"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY5761801.1"
                     /translation="MELAKAFEPADIESRWYPTWENAGHFAAGLDRSKPAENAFCILL
                     PPPNVTGTLHMGHGFNQTIMDALTRYYRMRGHNTLWQPGTDHAGIATQIVVERQLDAQ
                     GISRHELGREKFLEKVWEWKEYSGNTITRQMRRMGTSPDWTRERFTMDAGLSQTVTET
                     FVRLYNEGLIYRGKRLVNWDPKLGTAVSDLEVLAEEEDGFLWHIRYPLADGSGSLTVA
                     TTRPETMLGDTAVMVHPEDPRYQHLIGKAVKLPLCEREIPIVADAYVDLEFGTGCVKV
                     TPAHDFNDYAVGQRHQLPMISILTLDAKINDNAPAKYRGLDRFDGRKAVVADLDALGL
                     LEKTDKHKLKVPRGDRTGVVIEPMLTDQWFVAMTKPGQDGQSITQKALDVVASGEIKF
                     YPENWVNTYNQWLNNIQDWCISRQLWWGHQIPAWYGTDGRCWVAHDESEAKTLAAKDG
                     YTGALTRDPDVLDTWYSSALWPFSTLDWTPEWPAKSNDAIDLYLPSSVLVTGFDIIFF
                     WVARMVMMTRHLTGKIPFRHVYVHGLIRDAEGQKMSKSKGNVLDPIDLIDGIVIDELV
                     KKRTTGLMNPKDAPKIEKRTRKEFADGIPAYGTDALRFTFASLASPGRDIKFDLARCE
                     GYRNFCNKLWNASRFVLMNCEGEDCGLDDHPAGTCAPGGYLDFSFADRWIVSHLQRVE
                     AEVAQHFAEYRFDLLAQAIYRLVWDEFCDWYLELAKVQIQSGTPEQQRATRRTLIRVL
                     ETILRLAHPLIPFITEELWQTVAPLAGRKTHQSIMLAAYPEAQPEKHDESSEAKLALL
                     KQLASAVRNLRGEMNLSPAQKVPLLVEDKSAALTAFVPYIAALAKLSEVSVVPALPTD
                     AAAPVAVVGETRLMLKIEIDLAAERERLDKEIARLTNEVSKANAKLGNEQFVGRAPAN
                     VVAQEKERLAGFLATLDKLTGQRDQLPKA"
     gene            complement(10840..11268)
                     /locus_tag="VIS73_01195"
     CDS             complement(10840..11268)
                     /locus_tag="VIS73_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761802.1"
                     /translation="MSDDRQSGDDPLVNRIDALMRKRRSFVAADAASARHPEEEDIPE
                     LTEIVDLSQLSDASTEGGTLPIEPIIQALANELAHAIEYRLKTDLPGVLDSAIDKLAL
                     DLRRGVAGVLEAAVLDFLGRRQQLRLPFPDTPPGKPPSGD"
     gene            complement(11265..11702)
                     /locus_tag="VIS73_01200"
     CDS             complement(11265..11702)
                     /locus_tag="VIS73_01200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEY5761803.1"
                     /translation="MTRVDFYHNAGSRLETAARIVRKAYHQGVPALIYAPEPALAREL
                     DQLLWVAPSGSFLPHCGADDPLAGETPVVIAQDLASDRAPPNDKLLINLSAEVPPGFA
                     RFERIIEVVSTDGEAREQGRARFRHYRDRGYEINTHDVAGASA"
     gene            complement(11707..13206)
                     /locus_tag="VIS73_01205"
     CDS             complement(11707..13206)
                     /locus_tag="VIS73_01205"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY5761804.1"
                     /translation="MEFSIKSGSPEKQRTACVVVGVFQPRKLTLAGELLDKAARGYLS
                     ELLRRGDMEGKAGSVLLLHKIPGILAERVLLVGLGKEKEFGEKAYRDSVALAVKTLNE
                     TGSFDGTVFLTEVPLRKRDVAWRVRQAAIVARDVTYRFDQFKSKKEELRRPLRKLTFA
                     VDSRTELGSGERALAEGAAIAEGIALAKTLGNLPANVCTPTYLAERATQMAAEYGLDC
                     QVLEREEMESLGMNTLLSVARGSTQPPKFIILAYRGGKASEKPITLVGKGVTFDTGGI
                     SLKPAPEMDEMKYDMCGAASVLGTMLACAQLKLPINLTVLVPATENMPSGSATRPGDI
                     VTSLSGQTVEILNTDAEGRLILCDALTYAERLDPAVVIDVATLTGACVIALGHVATGL
                     LGNNDGLARELLAAGEDAYDRAWQLPLWEDYQEQLKSNFADMANIGGRAGGTITAACF
                     LARYTKKFDWAHLDIAGTAWKSGNDKGSTGRPVALLSQFLLKRAEKSRG"
     gene            13287..14372
                     /gene="lptF"
                     /locus_tag="VIS73_01210"
     CDS             13287..14372
                     /gene="lptF"
                     /locus_tag="VIS73_01210"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEY5761805.1"
                     /translation="MVFRRAFQRELTQTAVGIFVALFAILVTTQTIRLLNEAAGGKLA
                     PEAVLALLGFSAMHSLPILLSLTLFVATLLVVSRMHRDSEMVVWACSGLPLTGWIVPV
                     LRFALPVVVAVAAISLFVSPWAAAKSAEFRQKINNRESVAQLSPGMFRESSSGERVVF
                     VESHSNVQDRVANVFASSVQHGRLGVMVAREGYREEAPNGDGFLVLDQGRRYELTPGS
                     ADFRLLEFSRYAIRIEPGQVSDTATSPKAIPSIDLFRSDGRVERGELAWRLGLPISAA
                     LTVILALPLGFVNPRGGRSANLIVALLAFLAYNNAMGIVQTMIWTEKLPFSVGVWIVH
                     AGMALVVLAIFVHRATSSTKPWRRLWA"
     gene            14363..15454
                     /gene="lptG"
                     /locus_tag="VIS73_01215"
     CDS             14363..15454
                     /gene="lptG"
                     /locus_tag="VIS73_01215"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEY5761806.1"
                     /translation="MGLIALRYERYLGRELTVATSLVLLAFLGLFAFFDVINEIRFVG
                     QGNYSVGHAMAYVVLRLPGRVYELIPICVLIGALFALANLARHSEINVLRVSGVSTTQ
                     LLGALFKTAGLFALLTLLVGEFVTPLSERFAQQLKVRATSAVVGQEFRSGLWFKDGLS
                     FVNVRHVLPGARLQQVRIYRFDDALRLESVTDAQDGDYLPPDTWRLRSVVVSRLEEGG
                     LQADVSRSSEMLWKSALTPDILSVLSIVPERMALNHLVAYIDHLSANRQKTDRYEIAL
                     WKKLIYPLAALVMVALALPFGYTHNRVAGASVKIFTGVMIGILFHMLNGMFSSLGVIH
                     AWPPLASAAAPSVLFLLAAAAMLWWVERR"
     gene            complement(15451..15870)
                     /locus_tag="VIS73_01220"
     CDS             complement(15451..15870)
                     /locus_tag="VIS73_01220"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY5761807.1"
                     /translation="MSSAGLGRRLLSLVYEFFLLAGVWLSLVLFPQAIAASVFATAAP
                     GWLIWLHGFVIFAAMYCWLWSDGRRTLPMKTWRLRLVTDEGRDLQAVHAAMRYFWAWP
                     SYGLLGIGIAWAVFDAEGKFLHDRLARTRLLAETPPA"
     gene            complement(15867..16271)
                     /locus_tag="VIS73_01225"
     CDS             complement(15867..16271)
                     /locus_tag="VIS73_01225"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEY5761808.1"
                     /translation="MASTRHALLIALALFATGASANPPVTVAATPPQAAWADLSIEHR
                     TILSPLGNDWNELEHYQRKAWLGIAERWPQLNREERNRAWDRMRAWAKLSTDERREIR
                     ERYRRYQELSPAERARLKEQWENVSGRAGGGQ"
     gene            complement(16244..16621)
                     /locus_tag="VIS73_01230"
     CDS             complement(16244..16621)
                     /locus_tag="VIS73_01230"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HEY5761809.1"
                     /translation="MNQDDKFPDLIRKNLDRGLLELPPDTVRRLDDARHRALASRNSA
                     AGQVALAGNLELTLGLWAFRPRVFGFLSVALLVLATLAFLHGQRHIHQIEELDSAILT
                     DDLPLEAYLDKDFDQWLRRGTRY"
     gene            complement(16618..17190)
                     /locus_tag="VIS73_01235"
     CDS             complement(16618..17190)
                     /locus_tag="VIS73_01235"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY5761810.1"
                     /translation="MKALATRQELSEFLAATQRRAFRHAMFATRNEEASLDLVQDAMM
                     RLSDKYGDRPVEELPMLFQRILQNAIRDHYRRTRVRNTWTTLLSSLFPDGDDARDPLE
                     SIDTTGAPGQLQGPEDAFAQSRLMRIIEDEIGRLPARQREAFLLRYWEDLDIAETALT
                     MGCSEGSVKTHCSRASHTLAAALKRQGISP"
     gene            17308..19017
                     /gene="ilvB"
                     /locus_tag="VIS73_01240"
     CDS             17308..19017
                     /gene="ilvB"
                     /locus_tag="VIS73_01240"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEY5761811.1"
                     /translation="MMLTGAEILVRCLQEEKVEVVFGYPGGAVLHIYDELFKQDHTKH
                     VLVRHEQAAVHAADAYSRSSHRVGVALVTSGPGVTNAVTGIATAYMDSVPVVVITGQV
                     PTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLALTIKKAFHIASTGRPGPVVVDV
                     PKDITAHKCRFDGYPQTVQMRSYNPVVKGHLGQIKKAAQYLLDAKRPMLYFGGGVILS
                     NASEQLTKLARRLNIPVTGTLMGLGGYPSTDRQFLGMPGMHGTYEANMAMQHSDVLLA
                     VGARFDDRVIGNPAHFAENPRKIIHIDIDPSSISKRVKVEVPIVGNVPDVLEELLKLL
                     DASPDKQAPAHEDWWKEVEGWRSRKSLNYKQNGNLIMPQYVVQKLYEITKGDAIVTSD
                     VGQHQMWAAQYYQFDKPRRWLNSGGLGTMGVGLPYAIGATFANPDATVACVTGEASVQ
                     MCIQELSTIKQFRLPIKIICLNNRYLGMVRQWQEMFYGNRYSESYLDSLPDFVALAEA
                     YGHVGMRIERPEDVEEALRKAFVDHKDDCVFLDFITDQTANVFPMIAAGKGLTEMILA
                     EDL"
     gene            19031..19522
                     /gene="ilvN"
                     /locus_tag="VIS73_01245"
     CDS             19031..19522
                     /gene="ilvN"
                     /locus_tag="VIS73_01245"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY5761812.1"
                     /translation="MRHIISILIENESGALSRVAGLFSARGYNIETLTVAPTEDPSLS
                     RMTILTAGSDDVLEQITKQLNKLVDIVKVVDLSEAAHIERELMLIKVRATGKDREEMK
                     RMAEIFRGRIIDVTESTYVMELTGPSEKLDAFIGAIDKGLILETVRTGVCGIGRGDRI
                     LKA"
     gene            19545..20576
                     /gene="ilvC"
                     /locus_tag="VIS73_01250"
     CDS             19545..20576
                     /gene="ilvC"
                     /locus_tag="VIS73_01250"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY5761813.1"
                     /translation="MKGNTMKVYYDKDADLSLIKGRKVTIVGYGSQGHAHAQNLKDSG
                     VKVTVGLRKGGASWAKAEKAGLKVKEIADAVKGADVVMILLPDENIPSVYYAEVEPNI
                     KKGAALAFAHGFNVHYNQVVARLDLDVIMVAPKGPGHTVRSEYLKGGGVPSLIAVHQD
                     VSGKARDIALSYAAANGGTKGGVIETNFREETETDLFGEQAVLCGGAVELVKMGFETL
                     TEAGYAPEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGPEVINEQSR
                     EAMRNALKRIQTGEYAKMFILEGRTNYPAMTARRRLMAAHPIEQVGGQLREMMPWIKK
                     NKLVDQSKN"
     gene            20664..21326
                     /locus_tag="VIS73_01255"
     CDS             20664..21326
                     /locus_tag="VIS73_01255"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEY5761814.1"
                     /translation="MNYPHPIIAREGWPFLAIALIAAIVATYAGGWLLASPVWIIALF
                     VLQFFRDPAREIAGGPTSVVAPADGRIVAVETVEDPYLGRQALKVSVFMNVFNVHSNR
                     SPVDGLVVGRWYAAGSFLNAALDKASVENERCALHLRTAAGLDVTCVQIAGLVARRIL
                     NYSREGDSLARGERFGFIRFGSRVDLYLPLQTSVKVAIGEKVYASSTVLAELPAPAAT
                     NA"
     gene            21319..22101
                     /gene="pssA"
                     /locus_tag="VIS73_01260"
     CDS             21319..22101
                     /gene="pssA"
                     /locus_tag="VIS73_01260"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEY5761815.1"
                     /translation="MPEEPNSRKALFNPEVRRKGIYLLPNLLTTGALFSGFFAIVRAM
                     QGDFEAAAMAIFVAMVLDGLDGRVARLTRTQSAFGAEYDSLSDMVSFGAAPALVMYEW
                     ALRDLGRIGWIAAFIYCACAALRLARFNTTLEVTDKRFFQGLPSPAAAALVAGLVWVM
                     IDNGISGSDIPWLACVLTVFAGVTMVSNIRYYSFKDVNLRRSVPFIVVAGVALAFALT
                     ALKPDATLFAVFVTYALSGYVLAIWQRFRPAKDVGSSDSGSA"
     gene            22338..23879
                     /locus_tag="VIS73_01265"
     CDS             22338..23879
                     /locus_tag="VIS73_01265"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY5761816.1"
                     /translation="MKDRLIIFDTTLRDGEQSPGASMTRDEKLRVARQLERMKVDVIE
                     AGFAAASPGDFDAIRAIAEAVKDSTVCSLARANENDIRRAGEAIRPARSKRIHTFIAT
                     SPIHMEKKLRMTPEQVVEAAIRAVRLAGEYTDDIEFSAEDAVRSDMDFLCRVFQAVIE
                     AGATTINVPDTVGYSVPSVWGERMRELIERVPNSGRVVWSTHCHNDLGMAVANSLSAV
                     LAGARQVECTVNGLGERAGNASLEEVVMAVRTRSDIFPVETGVDTSQIVPASKLVSQI
                     TGYPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVGWSQNKLVLGKHSGR
                     NAFKTRLNDLGVVLASDEALNSAFARFKELADRKHEIFDEDLQALVSDERVTPEDEHF
                     KLIYSRVCSETGEMPESNLTMSVGGVEKRATASGGGPVDATFRAIEAVVGSQSELLLY
                     SVNAITTGTDAQGEVTVRLARGGRIVNGQGADTDIVVASAKAYLNALNKLEIHPEKLN
                     PQGDV"
     gene            complement(23876..24295)
                     /locus_tag="VIS73_01270"
     CDS             complement(23876..24295)
                     /locus_tag="VIS73_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEY5761817.1"
                     /translation="MSASAELPSSNPRPSPFRAWATLCLCALIALALLWELWLAPLRP
                     GGSWLVLKALVLALPLAGVIAGRVYTYQWASMLILLFVLEGTVRLMSDTGLTRVLAAI
                     ELLLSLLFFITAVAHIRASRGPRPPRGRRSASDRPET"
     gene            24353..25162
                     /locus_tag="VIS73_01275"
     CDS             24353..25162
                     /locus_tag="VIS73_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEY5761818.1"
                     /translation="MIDYFSRLFRRFREERFAQLAASLSFTTLLSLVPLVAVVIIIGN
                     RLPWIGGLVGYLDNFVIENLLPAQTGDVVARYAYRFSRQANRLTFGGAVLLLVASTAL
                     MFSIERAFNHLWRVRQPRSLAERIFLYTIAIAVFPLLAGGLLGISAFLVNAAMPLAGQ
                     REWARYLAVHGSAGMLLWGLFAVLYASVPACQVRWRHALTGGAVAAIGVTAAQRLFGY
                     AIDQASVYKTLYGAFSVLPIFLVWIYLCWAIVLVGALVAANFAGPGPLRRR"
     gene            complement(25181..26671)
                     /locus_tag="VIS73_01280"
     CDS             complement(25181..26671)
                     /locus_tag="VIS73_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761819.1"
                     /translation="MALPEHRSAQSADANAQPVLAWLATPPSEEVDHDIDRLRSYVRA
                     VAENEFSPALRQRLTTQIQDRADRIVEQAFANLRKTRLPLSVRVRTLVRRLQDALETM
                     AHLYFGEIGSTERLIKGLQRPPEQSLWLALDSLRKHQLLSNLAAAPHGPEIWRIAHDI
                     RAKASSMNLLDAIPPERSASIGTQYARFLLAGCPHPATFTSAEWCFIDRYLADLASTI
                     SLGNQPPSREEGQFWVNLKNDMPPVAFARKPPPIGSDCLYFDVASIGAAINDRLASSD
                     EESRRALALLADDLPMGAALAALQRLRNLLSDTRKRRFSRRRQGYRARICFRLDAIWQ
                     LLRGQRDDDELGEWQVVNESPDGYATMHVTGKTQKLQVGDLAAIRRYHDEDWQICIIR
                     WAQSENPEHFELGLQALAPRAVGGMLALPGPEEISKQPVLVMAPIAGQGNSTLAVPAG
                     LVLDPQTSHVIITEGPNVGIREIRLERRVESTAGTDLYLIASGSAG"
     gene            26828..27082
                     /gene="rpsP"
                     /locus_tag="VIS73_01285"
     CDS             26828..27082
                     /gene="rpsP"
                     /locus_tag="VIS73_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY5761820.1"
                     /translation="MVVIRLSRGGAKKRPFYSMIVTDSRNRRDGRFVERIGFYNPMAA
                     ENELGLNVNVGRLEFWKGQGAQLSPTVARLVKQFKPQQAA"
     gene            27129..>27682
                     /gene="rimM"
                     /locus_tag="VIS73_01290"
     CDS             27129..>27682
                     /gene="rimM"
                     /locus_tag="VIS73_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766625.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY5761821.1"
                     /translation="MSSSEAGLPVGANAGPYVVLGRVSGAYGVRGWLRIQVFGDDPLS
                     WREMDTWWLSRSRDRLTNDPSSDEDWEAYGLGQCRLQGGDLVAQLTGIADRDAAEALK
                     GALLAVPRAHLPDPGKDAYYWGDLLGMAVVGTGNVPLGVVVRLLETGANDVLVVIDAD
                     GHERLLPFVESVIARVDPAVRRIDA"
BASE COUNT         4675 a         8474 c         9072 g         5461 t
ORIGIN      
        1 ctatgcgccc gggaacgccc gattcgatgc gatcggcaaa gcgtagtacg accccgttgt
       61 tagccgagag cacacgcgcc gtttcactgc gctcctcgcc ggtcgcggcg ttggtccgaa
      121 cgacggtgac ttccttgccg acgtacttct cgagaagctt ggccggcgtc aggaggtcga
      181 aatcgaagtt ttgttcgacg agggtaatcg gagcgccgct ggccgcgcgc agcagggcgg
      241 tttcgggtcg catctgcgct gatacatcgc gcagcgccag gcggctcatg ccggcgggca
      301 gtgtcacctt acgtttctcc ttcactagcg cgaggtcgtc gttgtagacg gtaacggctg
      361 tggactcgcg gtcgctgccc tgcgacacgg tttcgcggat cgaatcggcg gccggggcgg
      421 ttcccggcag cagcgcggcc aggcaaagcg ttgccagatg gcgggcggcg atcggcgacg
      481 gccgttgcgt cttcatgcgc ggtctcctca atgggggggc agaatagcat cggcaaattg
      541 gcgcatcggc aggtgcgggg cggctttaag gatgccgtgg ctggtcaaaa agaaacgggc
      601 gctgcggcgc ccgttgccgg cagcaggctg cattcagacg gtgacggctt ccttgtcatc
      661 ggcaaagctg agcttgacga cgccgtcggc gtcgatgtcg acggtgatct tgccgccgtg
      721 cgacagcttg ccgaacagca gttcgtcagc cagtgccgaa cggatggttt cctgaatcac
      781 ccgcgtcatc ggccgcgcac ccatcagcgg gtcgaagccc ttcttcgcaa ggtgttcctt
      841 gagcgcatcg gtaaagaccg cctcaacctt cttctcgtgc aactgctcct cgagttgcat
      901 gaggaactta tcgacgacgc gcaggatgac ttcgtggtca agcggacgga aggaaatggt
      961 cgcgtcgagg cggtttcgga actccggcgt gaacaggcgc ttgatttcgg ccatttcgtc
     1021 gccggccttc ttttcagtgg tgaagcccat cgtcgtcttc tgaagcgatt cctgccccgc
     1081 gttagtggtc atgatgatca tcacgttgcg gaagtcggcc ttgcgcccgt tgttatcggt
     1141 cagcgtgccg tggtccatca cctgcagcag gatattgaag atgtccggat gtgccttttc
     1201 gatctcgtcg agcagtagta ccgcgtacgg cttcttggtc acttcttccg tcagcaggcc
     1261 gccctgctcg aagccgacgt acccgggcgg cgcgccgatc agccgcgaca cggcatggcg
     1321 ttccatgtac tcggacatgt cgaagcggat cagctcgatt cccagcgaga aggcgagctg
     1381 gcgtgcgacc tcggtcttac cgacaccggt gggcccggaa aagaggaagc tgccgattgg
     1441 tttgcccgga ttgccgagcc ccgaacgcgc catcttcacg gccttggcca gcgcgtcgat
     1501 cgccgggtct tggccgaaga cgactgcctt caggtcgcga tcgaggttct tcagcgagga
     1561 cttgtcgtcg cttgagacgt gttgcgacgg aatgcgtgcg atcttggcga cggtttcttc
     1621 gatctcgggt ttgccgatcg tctttttctg cttcgacttg ggcaggatac gttgcgcagc
     1681 accggcctcg tcgatcacgt cgattgcctt gtcgggcaga tgtcggtcgg tgatgtagcg
     1741 cgccgacagc tcggctgccg cggtcagcgc cgccgccgtg tacttgatcc cgtggtgcgc
     1801 ttcgaaacgc ggcttcaggc ccttgagaat gtgtatcgtc tcgctgaccg agggctcgac
     1861 cacgtcgatc ttctggaagc ggcgcgatag cgcgtggtcc ttctcgaaga tgccgcggta
     1921 ctcggtgtag gtggtggcgc cgatgcactt tagctggccc tgcgaaagtg ccggcttgag
     1981 caggttggac gcgtcgagcg tgccgcccga ggcggcgccg gcaccgatca gtgtgtggat
     2041 ctcgtcaatg aagaggatcg cgttgggatt gccggcgagc tgcttgagca cgcccttgag
     2101 gcgttgctcg aagtcgccgc ggtacttggt tccggcaagc agggcgccca tgtccagcga
     2161 atagacattc gcctcggaaa ggatgtcggg aacctcgctc tcgacgatgc gccgggccag
     2221 cccctcggcg atggcggtct tgccgacgcc ggcctcgccg actagcaggg gattattctt
     2281 gcgccgacgg cacagcgtct gcacgacgcg ctcgagttca cggtcgcggc cgatcagcgg
     2341 atcgatcttg ttttgcagtg ccagcgcgtt gaggttgatc gtgtagctct ccagcggccc
     2401 ggcggcgttt ccttcggccg attccttctc ttcctcgtgt tctggcgctg ccttgggctg
     2461 ctgcaccttg ctgatgccgt gcgagatgaa gttcacgacg tcgagccggg tgatgccgag
     2521 cttgtgcagg taatagaccg cgtgcgagtc cttttcgccg aagatcgcca ccagtacgtt
     2581 ggcgccgttg acttccttct tgcccgagga ctgcacgtgc aggatggcgc gctggatgac
     2641 gcgctggaag ccgagggtgg gctgggtctc gatgtcgttg tcgccggaga ccgtcggcgt
     2701 gtgctggccg atgaactggg tcaggtcctt gcgcagggtc tcgacgttgc cgccgcaggc
     2761 gcgcagggcc tcggctgccg acgggttgtc gagcagtgcg agcagcagat gttcgacggt
     2821 gatgaactcg tgccgcttct gccgcgcctc cacgaacgcc atgtgaaggc tgacttccag
     2881 ttcttgcgcg atcattcagc tttcctccat gacgcatgcc agcggatgct ggtgctcccg
     2941 cgcaaacgag agaaccagct cgaccttggt tgcggcgatg tctcggggga agaccccgca
     3001 cacgccacga ccttcgttat ggactttgag catgatacgc gtcgcttgtt cgcggtccat
     3061 tgaaaaaaaa cgctggagca cgaggacgac gaactccata ggtgtgtaat cgtcattcag
     3121 cagcagaacc ttgaacatcg gcggcagcgc ggtccgcgtc cgttccgctt ctagtacgct
     3181 tttttgggtc ttggcagcca tgatccattc tataaacagc gattcggcac cggcaagttt
     3241 ggcaggtgcc ggcgccgggg tttctggtca gtgcctctgc cgagcatcgg cgcctggggc
     3301 gggtaccgta catctccgtc atgccgtcga ttttgcagga ttcgagcggt gcctgaagaa
     3361 agcttgggac tttgcttgac ggccctattg aaacggcgta caaagcggcg tgtttggctt
     3421 caagaagtag ctggtcgtcg taatgcttcg ccctatggac gagaacgtga cgcggtcggg
     3481 tcagatctgg agttaaacat ttacgcgggc aatgcccgga tctacgtttc tcaagaaggg
     3541 aatgcagcaa tatggcaatc ggtacggtca agtggttcaa tgatgcgaag ggctttggtt
     3601 tcattacgcc ggacgacggc agtgaagacg tcttcgctca cttctcggcg atcaacatga
     3661 acggcttcaa gagcctgaag gaaggcgaaa aagtctcctt cgaagtcacg cagggcccga
     3721 agggcaagca ggcgaccaac attcagcgcg cctgatcgcg actcgaagag gtcattgaag
     3781 gcccgccttg gcgggccttt ttcattgtcg ctgcctgggt cttcggcccg cgtcagggcc
     3841 tttgccaagt tcctgacttg ccgcccgatt tctcgatcag gccgatcgat tcaatgacca
     3901 tgccgcggtc gattgccttg cacatgtcgt agatcgtcag cagtgccaca ctcgctgccg
     3961 tcagcgcttc catctcgacg ccggtgcggc cgacgcattc ggtggtggcg gtgcagttga
     4021 ccgaatgggc ggcagtgtcg atctcgaaat cgacggcgac atgagtgagt ccgatcgggt
     4081 gacaaagtgg aatcaggtcg cccgtccgtt tcgcggcctg gatacccgcc agtcgggcta
     4141 tgccaagcac gtcgcccttg tcggcgcgac cgtcgacgat gcgctgcaac gttgcggctt
     4201 gcatgacgat ccgcccgccg gcgcgcgcga gacggcgcgt ctcggccttc tcagcgacgt
     4261 caaccatgtg ggcgcggcca gcgttgtcga aatgggtcag cgtcgggtcg gtcatcaatg
     4321 ctctcggcgg gttggcccgg cgacgcgatc cctatgggcc gcattcacaa aaacggcctt
     4381 ctggtcggcc tttcgggcag gtcggttggt atcatagcac catgaaacca cggttcattg
     4441 cggccactgc ggccagttgc ctgctgctcg cgctcggtgc cggattcgcc ggtgccgaca
     4501 accttcctga tcttggcgat gcgtcggcgc tcgacgttcc gccagcgctc gagcgtctgg
     4561 tcggcgaggc gcagatgaac gagattcgtc tgcgcgagcc gcgcttcctc gacgatgccg
     4621 aactcacttt ctacctgcag gggctggcca accggctgct agcggcgggc gacacggccg
     4681 gcgtcgagat ccggctgttc gcgctcgacg atccatcgat caatgccttc gcaatgttcg
     4741 gtggctatat cggcgtcaat actggcctga tcacggcggc gcaaagtgag agcgaggtcg
     4801 ccggagtgtt ggcgcacgag attgcccacg tctcgcaacg gcatctggca agacaggttg
     4861 cggcgcagaa acaggttgcg ttaggcagca tgctggcgat cgctgtcgcg atctttgcgg
     4921 cacgctcgaa cgccgaggtc gccaatgccg cgctggcgac ctcgcaggcg gccggcatcc
     4981 aggcacagct aggtttctcc cgcgatcacg agcgcgaggc cgatcgcatc gggttcgaga
     5041 tgatgcagcg ctcggggctc gattcgcggg cgatggtcag tttcttccag cgcctgcagc
     5101 gtgctggtcg cgtctacgag aacaacgcgc cgacctatct gcgaacccac ccgctgacgc
     5161 tggatcggat taccgacctc gaacaccgtg tcgcgcaggc tgcgccctat gcacagcgga
     5221 acggaggagc cagcgagttt ccgttgatcc aggccaagct cttgtcgatg cgcgggccga
     5281 cagccgatgc gctcgaaggg gcgcgcgacc gttacgacaa ggctgccgac aagcccgtct
     5341 cgtcttatgg gctggcagta gccctgacgc gcactctgca gttcgacgag gcggcgcgcg
     5401 tcatcgctga tgccgatgcg cggtcgccct tggtgcagcg cctcgcggcc gaaattgccg
     5461 ccgcgcgcgg ccagcgcgac gaggccgagc agcggtaccg gcaggcgctg gcggcgcacg
     5521 gggagcggcc agcgctctgg tacggacttg ccgatctgtt gctggcgtcc ggccaacacg
     5581 agcaggcgcg agaactgctc gaccggcgcc tgcgcaaggt caacgacgac cccgatgcgc
     5641 atgcccggct ggcgcgggcc tatgaaggcc tgggccgcat cgcttcgcgc catcgtgctc
     5701 aggccgaggc ctaccagttg cgcggccaga cgctggcggc gatcgagcag ctggagttgg
     5761 cgcagcgagc cggaaccagc gacttcgtcg agcagtcgat gatcgacgcg cgcctgcgcg
     5821 aactcaagcg gcgacagtcc gaccgggcgc cagacgcacg gaaatgagcg aaggccagcc
     5881 gaccgcagat cgcgagattg atgtccgcgg cctgcattgc ccgtatccct tgctagccgc
     5941 caagaaagcg ctggcaacga tgtctgccgg cgagcatctg cgggtgctgg cgaccgaccc
     6001 gaatgcagca aaggacttcc ggttgtttgc tgaggccagc ggcaacgcgc tggtgttcgc
     6061 gggcggcgag gcgggcgcgc tgaccttcgt ctttcgtcgg cgctagcggc gacgccggcc
     6121 aatcggcatg tccgccgttt ccatcgaagc gctcgaacca ttcgtcctgc tcgaaacgcc
     6181 ggaaacgctg cgcgtattcc ggcggcaact cgaggccgcg ccggtttctt gcgccgacga
     6241 tctggagcgg ctgctgggtc cggataccgc gtatcgggtt gttgcgttcg attacgaact
     6301 ggggcattcg atcgagccgc gcagcggtga ttgcgccagc ggtgcggtcg gcacggtctg
     6361 gcgttttgcc gaatgcgatg agattccgct tgccgagtgc gatgcctaca tcgagcggca
     6421 cgtcgcggtg ctgccggccg acgcgcgggc agcggccgtt gccgacctcg cgatcggtct
     6481 cgacgaggcg gcctacgttg ccggggtcgc gcgaatccag cgctggatcg ctgatggcga
     6541 ctgctatcag gtcaattaca gctttcccct tcacctgcgc acgatcggcc atccgctggc
     6601 gctgtacgcc gcgttgcgca ggcgtcagcg cggtctctgg tcggcctgca tcctgaccgc
     6661 cgagcggcga attctttcgt gttcgcccga actgtttgtc gccaagcgcg gcgacgaact
     6721 ggcgtgccgg ccgatgaaag ggacgcgccc gcgcagcgct gatgctgccc gcgatgccgg
     6781 gaatcgcgcc gaactcgatg cagcggagaa ggaccgcgcc gagaacgtga tgatcgtcga
     6841 cctgctgcgc aacgacctgg gccgtgtcgc ggtgccgggg agcgttcggg tcgatcgact
     6901 gttcgaggtc gaaagctacc ccaccgtgca tcagctggtg tcgtcgattc gcgccaaggc
     6961 gccggggcgg cggatgcccg agatcgtgcg ggcgctcttt ccctgcggct cgattaccgg
     7021 tgcgccgaaa ctgcgcgcca tgcagatcat cggcgcgctc gaaaccgcgc cgcgcggcat
     7081 ctataccgga gccatcggct gcctgatgcc cgatgacgac ttcacgctca acgtcgccat
     7141 tcgcacgctc gatcttgaag ccggcgggcg ggcgcggctc ggtgttggca gcggcatcgt
     7201 cgccgattcg gtcccggccg acgagtatcg cgaatgcctg ctcaagggtg agtttgcgac
     7261 cgggctgccg gcgccgtttt cactgatcga gacgctgcgc ctcgaaaacg gtgcgctgcc
     7321 tcgcctggcc ggccatctgg ctcgcctggc cgactcggcg cgacggctcg gatttcgcta
     7381 tcccgcctac gagatttctg cggccctgca ggcgcgtgcc gccgagaatc cgctgggcgt
     7441 ttttcgtttg cgcttgttgc ttgccccgtc gggcagcgtg tcaactacag tgcagccctt
     7501 cgtgccgctt ggcgatgacc ggcgcatcac gctggccgac gggcgcctcg attcggccga
     7561 ctggggcctg cgcttcaaga ccacgcggcg ggcgcgctac gacgaggcac tgcgtcgtct
     7621 accgccggag gtcttcgata ccgtgtttct caacgagcgt gacgaactgt gcgaaggcgc
     7681 ccgcagcaac ctgttcctgc tgatcgaagg gcgcctgctg acgccggccg tcgactgcgg
     7741 attgctgccc ggtgttctgc gtgccgagct gctggcgagc ggtcgggccg ccgaagccgt
     7801 gctcgggctt gacgacctgc ggcgagccga ggcgatctac gctggcaacg cgctgcgcgg
     7861 cctggtgtcg gtgacgctgg ccaccgattc ctgaagcctg tcggcccgtg cggtccgctc
     7921 aggccttcgg cagctggtcg cgttgtccgg tcagcttgtc gagtgtggcg aggaagccgg
     7981 ccaggcgctc cttttcctgc gcgacgacat tggccggtgc tcggccgacg aattgctcgt
     8041 tgccgagctt ggcgtttgcc ttgctgactt cgttggtcag gcgcgcgatc tccttgtcga
     8101 gtcgctcgcg ttcggccgcc aggtcaattt cgatcttcag catcaggcgc gtttcgccaa
     8161 cgaccgccac cggggcggcc gcatcggtgg gcaatgcggg aaccaccgag acctcggaga
     8221 gcttggccag tgctgcgata tacggcacga aggcggtcag cgcagcgctc ttgtcctcga
     8281 ccaacagcgg caccttctgt gccggtgaca ggttcatctc gccgcgcagg ttgcgcacgg
     8341 cgctagcgag ctgcttgagc aatgcgagct tggcctccga gctctcgtcg tgtttttccg
     8401 gctgcgcctc ggggtaggct gcgagcatga tcgactgatg ggtcttgcgc ccggccagcg
     8461 gcgcgactgt ctgccagagc tcctcggtga tgaaggggat cagcgggtgg gcgagacgga
     8521 ggatggtctc gagcacgcga atcagcgtcc ggcgcgtggc gcgttgctgc tccggcgttc
     8581 ccgactggat ctgcaccttg gccagttcga gataccagtc gcagaattcg tcccagacca
     8641 gccggtagat cgcctgggcg agcaggtcga agcgatattc ggcaaagtgc tgcgcgactt
     8701 cggcttcgac gcgctgcagg tgcgagacga tccagcggtc agcgaaagag aagtctagat
     8761 agccaccggg ggcgcaggtg ccggccggat gatcgtcaag gccgcaatct tcgccctcgc
     8821 aattcatcag cacgaagcgg ctggcgttcc acagcttgtt gcagaagttg cggtagccct
     8881 cgcagcgggc gaggtcgaac ttgatgtcgc gacccggcga ggcgagcgag gcaaaggtga
     8941 agcgcaaggc atcggtaccg taggcgggaa tgccatcggc gaattccttg cgcgtgcgct
     9001 tctcgatctt cggcgcgtcc ttgggattca tcagaccggt ggtgcgcttc ttcaccagtt
     9061 cgtcgatgac gatgccgtcg atcaggtcga tcgggtcgag gacattgccc ttcgatttcg
     9121 acattttctg gccttcggca tcgcggatca ggccatgaac atagacatgc ctgaagggga
     9181 tcttgccggt caggtgccgc gtcatcatga ccatgcgcgc gacccagaag aagatgatgt
     9241 cgaaaccggt gaccagcacc gaggagggca ggtagaggtc aatcgcgtcg ttcgactttg
     9301 ctggccactc gggggtccag tcgagcgtcg aaaacggcca cagcgctgac gagtaccagg
     9361 tgtcgaggac gtccgggtcg cgggtcagcg cccccgtgta accatccttg gcggccagtg
     9421 ttttcgcttc ggattcgtca tgggcaaccc agcagcggcc gtcggtgccg taccaggcgg
     9481 ggatttggtg gccccaccag agctgccgcg agatgcacca gtcctggatg ttgttgagcc
     9541 actggttgta ggtgttgacc cagttttccg ggtagaactt gatctcgccc gaggcgacga
     9601 cgtcgagcgc cttctgcgtg atcgactggc cgtcctggcc cggtttcgtc atggcgacaa
     9661 accactggtc ggtcagcatc ggctcaatga cgacgccggt gcggtcgccg cgcggcacct
     9721 tgagcttgtg cttgtcggtc ttctcgagca ggccgagggc gtcgaggtcg gcaacgacgg
     9781 ccttgcggcc gtcgaagcgg tcgaggccgc gatacttcgc cggcgcgttg tcgttgatct
     9841 tcgcgtcgag cgtgaggatg gagatcatcg gcaactgatg gcgctggccg accgcgtagt
     9901 cgttgaagtc atgtgccggc gtgaccttga cgcaaccggt gccgaattcg agatcgacgt
     9961 aggcgtcggc gacgatcggg atttcgcgct cgcacaaagg tagtttcacc gctttgccga
    10021 tcaggtgctg gtagcgcggg tcttccgggt gaaccatgac cgcggtatcg ccgagcatgg
    10081 tttccggtcg ggtggtcgcc accgtaagcg aacccgaacc gtcagccagc ggatagcgga
    10141 tgtgccagag gaagccgtct tcctcctcgg cgaggacttc aaggtcggaa acggcggtgc
    10201 ccagcttcgg gtcccagttg acgaggcgct tgccgcggta gatcaggcct tcgttgtaga
    10261 gccgcacgaa ggtttcggtg acagtctgcg acagccccgc gtccatcgtg aaacgctccc
    10321 gcgtccagtc ggggctggtg cccatgcggc gcatctgccg tgtgatggtg ttgcccgaat
    10381 attccttcca ctcccagacc ttctcgagga atttttcgcg gccgagttcg tggcgggaga
    10441 tcccctgagc atcaagttgg cgctcgacga cgatctgcgt ggcgatgccg gcgtggtcgg
    10501 tgcccggttg ccagagcgtg ttgtggccgc gcatccggta gtagcgggtc agcgcgtcca
    10561 tgatcgtctg attgaagccg tgccccatgt gcagcgtacc ggtaacgtta ggcggcggca
    10621 gcaggatgca gaaggcgttc tcggcgggtt tcgaccgatc gaggccggcg gcgaaatgac
    10681 cggcgttctc ccaggtcggg taccagcggc tttcgatgtc cgccggttca aaggctttgg
    10741 ctaattccat ggggaagcgc tcactgcgtt gcggcaaaag ccgcggatta tagaggagcc
    10801 ccccgtgccg ccggggccct gcgctgccgg ccatgggcct cagtcgccgc tgggcggctt
    10861 gccgggtggc gtgtcgggga agggtaggcg gagttgctgc cgccggccaa ggaaatcgag
    10921 caccgcggct tccagcacgc cggcgacgcc ccggcgcagg tccagtgcca gcttgtcgat
    10981 tgccgaatcg aggacgccgg gcagatcggt tttcagccgg tactcgatgg cgtgagccag
    11041 ttcgttggcc agtgcctgga tgatcggctc gatcggcagc gtgccgccct cggtcgaggc
    11101 gtcgctgagc tggctgagat cgacgatctc ggtcaactcg gggatgtcct cttcttccgg
    11161 gtgccgtgcc gacgccgcgt cggcggcgac gaagctgcgc cgcttgcgca tcagcgcgtc
    11221 gatgcggttg accagcgggt cgtcgccgct ttgccggtca tcgctcatgc cgaggccccg
    11281 gcgacatcgt gggtgttgat ttcgtagccg cggtcgcgat agtggcgaaa acgcgcgcga
    11341 ccctgttcgc gcgcctcgcc gtcggtcgac acgacttcga tgatccgctc gaagcgggca
    11401 aaccccggcg gaacctcggc gctcaggttg atcagcagct tgtcgttggg cggcgcgcga
    11461 tcgctcgcca gatcctgcgc gatgacgacc ggcgtctccc cggcgagtgg gtcgtcggca
    11521 ccgcaatgcg gcagaaaact gccgctcggt gcgacccaca gcagttgatc gagctcacgc
    11581 gccagcgccg gttcaggcgc ataaatcagc gctggcacgc cctggtggta ggcctttcga
    11641 acgatgcgcg ccgccgtttc aagcctgctt ccggcgttgt gatagaagtc gacgcgagtc
    11701 atccgctcag ccgcgcgatt tctcggcgcg cttcagcagg aactgcgaca gcagcgccac
    11761 cggccggccc gtcgagccct tgtcgttgcc cgatttccac gcggtaccgg cgatgtcgag
    11821 gtgcgcccag tcgaacttct tggtatagcg ggcaaggaag caggccgccg tgattgttcc
    11881 gccggcacgg ccgccaatat tggccatgtc ggcaaagttg ctcttcagct gctcctggta
    11941 gtcctcccac agcggcagct gccaggcgcg gtcgtaggca tcctcgccgg ccgcgagcaa
    12001 ctcgcgcgcc agaccgtcgt tgttgccgag gaggccggtg gcgacgtggc cgagcgcgat
    12061 gacgcaggcg ccggtgaggg tggcaacgtc gatgacgacg gccgggtcga gccgctcggc
    12121 gtaggtcagc gcgtcgcaga gaatcaggcg gccttcggcg tcggtattga ggatctcgac
    12181 ggtctggccc gaaagcgagg tgacgatatc gccgggccgc gtcgcgctgc cgctcggcat
    12241 attctcggta gcagggacca gcacagtgag gttgatcggc agcttgagtt gcgcgcatgc
    12301 gagcatggtc ccgagcaccg acgcggcgcc gcacatgtcg tacttcatct cgtccatttc
    12361 cggcgccggt ttcaggctga tgccaccggt gtcgaaggtg acgcccttgc caaccagcgt
    12421 gatcggcttc tcggaagcct tgccgccgcg ataggcgagg atgatgaact tcggcggctg
    12481 ggtcgagcca cgggccaccg agagcagcgt gttcatgccc agcgactcca tctcctcgcg
    12541 ttcaagcacc tggcagtcga ggccatactc ggcagccatc tgcgtggcgc gctccgccag
    12601 ataggtcggc gtgcacacgt tggccggcag gttgcccagc gtcttggcga gggcgattcc
    12661 ctcggcgatc gccgcgcctt cggccagcgc ccgttcgcca ctgcccagtt cggtccggct
    12721 gtcgacggcg aaggtcagct tgcgcagcgg gcgccgcaat tcctcctttt tcgacttgaa
    12781 ctggtcaaag cgataggtga cgtcgcgggc gacgatggcc gcctggcgga cccgccaggc
    12841 aacgtcgcgc ttgcgcagcg gcacttcggt gaggaacacc gtaccgtcga aggagcccgt
    12901 ctcgttgagg gtcttgacgg ccagtgcgac cgaatcgcgg taggcctttt cgccaaactc
    12961 cttctccttg ccgagaccga ctagcagcac gcgctcggcg agaattccgg gaatcttgtg
    13021 cagtagcagg acgctgccgg ctttgccttc catgtcgccg cgccgcagca gttccgacag
    13081 atagccgcga gccgccttgt cgaggagttc tccggcgagt gtgagcttgc gtggctggaa
    13141 tacgccgacc acgacacagg ccgtgcgctg cttttccggg ctaccgcttt ttatgctaaa
    13201 ttccacgcga atttcctttc tggcgtcaaa tcgggctggt ttcctgcatt tcgattatcg
    13261 ccaataccac cggaattcgt caaagcatgg tttttcgccg cgcgtttcag cgcgaactta
    13321 cgcagaccgc ggtcggaatt ttcgtcgccc tcttcgcgat tctggttaca acgcagacga
    13381 tccgcctgct gaacgaggct gccggcggca aactggctcc ggaagccgtg cttgcgctgc
    13441 tcgggttttc ggcaatgcat tcgcttccaa tccttttgtc gcttaccttg ttcgtggcaa
    13501 cgctgctggt tgtttcgcgt atgcaccggg actcggaaat ggtcgtctgg gcttgctcgg
    13561 gcctgccgct gactggctgg atcgttccgg tgctgagatt tgccttgccg gtcgtggtcg
    13621 cggtggccgc aatttcgctg tttgtttcgc catgggcggc agcgaagtca gcggaatttc
    13681 ggcagaagat caacaatcgc gaaagtgtcg cgcagctttc gccgggcatg tttcgcgagt
    13741 cttcgtccgg ggaacgggtc gtttttgtcg aatctcacag caacgtgcag gaccgtgttg
    13801 ccaacgtctt cgccagctcg gtacagcatg gcaggctcgg cgttatggtc gcccgcgaag
    13861 gttatcgtga ggaagcgccc aacggcgatg gttttctggt actcgatcag gggcgtcggt
    13921 atgagctgac ccccggctcc gccgactttc gcctccttga gttttcacgc tacgcgattc
    13981 ggatcgaacc tgggcaggtc agcgacaccg cgacttcccc gaaggccatt ccgagcatcg
    14041 acttgttcag gtcagacggt cgtgtcgagc ggggagagtt ggcttggcgt ctcggcctgc
    14101 cgatttccgc cgcgctgacg gtgatcctgg cgttgcctct tggcttcgtg aacccgcgag
    14161 gcggtcgttc ggcgaacctg atcgtcgcct tgctggcgtt cttggcctac aacaacgcga
    14221 tgggtatcgt gcagacgatg atctggaccg aaaagctgcc attttcggtt ggcgtttgga
    14281 tcgtccatgc cggtatggcg ttggtggtgc ttgcgatatt tgtccaccgc gcgaccagtt
    14341 ccaccaagcc gtggcgtcgt ctatgggcct aatcgcgctt cgctacgagc gttatctggg
    14401 gcgcgagctg acggttgcaa cctcgctggt gctgctggcc tttctcgggc tgttcgcctt
    14461 ctttgacgtc atcaatgaga tccgcttcgt cgggcagggc aattacagcg taggccatgc
    14521 catggcctat gttgtgctgc gcctcccagg gcgcgtctac gagttgattc cgatctgcgt
    14581 attgatcggg gcgctgttcg ccttggcgaa tctggcgcga cattctgaga tcaatgtgct
    14641 tcgcgtttct ggcgtttcga ccacgcaact cctcggagcg cttttcaaga ccgcggggct
    14701 gttcgcgctg ctgacccttt tggtcggcga attcgtgacg ccgctcagcg agcgatttgc
    14761 ccagcaattg aaggtaaggg caaccagcgc agtggtcggc caggagtttc gcagcggtct
    14821 gtggttcaag gatggtcttt ccttcgtaaa tgtgcgccat gtcttgcccg gcgcccgcct
    14881 acaacaggtc cgcatctatc gcttcgacga cgccttgcgc ctggaaagcg tcaccgatgc
    14941 acaggacggc gactacttgc cgccagatac ttggcgcttg cgttcggtcg ttgtttcacg
    15001 gctggaggag ggtgggctac aagcagatgt ctcgaggagc agtgagatgc tatggaaatc
    15061 cgctctaacg cccgacatcc taagcgtcct ttctattgtt ccagagcgga tggcgcttaa
    15121 ccatctggtc gcctatattg accacctcag cgccaacaga cagaagaccg atcgctacga
    15181 gatcgcgctt tggaagaagc tgatctaccc gctggcggca ctggtgatgg tggcgctggc
    15241 gctaccgttt ggttataccc acaatcgcgt ggcgggcgcc agtgtgaaga tctttaccgg
    15301 cgtgatgatc ggcattctgt ttcacatgct gaatgggatg ttttcaagcc tcggcgtgat
    15361 tcacgcctgg ccgccgctgg ccagcgcggc cgccccttcg gtgctgtttc tgctggcggc
    15421 cgccgccatg ctgtggtggg tcgagcggcg ctaggccggg ggcgtttccg ccagcagacg
    15481 ggttcttgcc aggcgatcgt ggaggaactt gccctcggca tcaaaaaccg cccaggctat
    15541 gccgattccg agcaggccgt agctgggcca ggcccagaaa tagcgcatcg ccgcgtgcac
    15601 tgcctgcaga tccctaccct cgtcggtcac cagccgtagc cgccaggtct tcattggcag
    15661 cgttcgcctg ccgtccgacc acagccagca gtacatggcc gcgaatatga cgaagccgtg
    15721 cagccatatc agccaaccgg gtgcggcggt ggcgaacacc gacgcggcga ttgcctgcgg
    15781 aaacaacacc agcgagagcc agacgccggc gagcagaaag aactcgtaga cgaggctgag
    15841 caggcggcgc ccgagccctg ccgaactcat tggccgccgc cggcgcggcc ggagacgttc
    15901 tcccactgtt ccttcaggcg tgcccgctca gcgggggaga gttcctggta gcgacgataa
    15961 cgttcgcgaa tctcacgccg ttcgtcggtg ctgagcttcg cccacgcgcg catccggtcc
    16021 caggcgcggt ttcgctcttc gcggttcagt tgcggccagc gttcggcgat gcccagccag
    16081 gccttgcgct ggtagtgctc gagttcgttc cagtcgttgc cgagcggcga gaggatggtt
    16141 cgatgctcta tgctgaggtc agcccacgcg gcctgcgggg gtgttgcggc aacggtcacc
    16201 ggcggattgg ccgaggcgcc agtcgcgaag agggccagtg cgatcagtag cgcgtgccgc
    16261 gtcgaagcca ttggtcgaag tccttgtcga gatacgcctc cagcgggagg tcgtcggtga
    16321 ggatcgcgct gtcgagttcc tcgatctggt gaatgtggcg ctgaccgtgc aggaaggcga
    16381 gcgttgcgag cacgagaagg gccaccgaca gaaacccaaa gacgcgcggc ctgaacgccc
    16441 acaggccgag cgtgagttcg aggttgccgg cgagtgcaac ctggcccgcc gccgaattcc
    16501 gactggcgag cgcacggtgg cgcgcgtcgt cgagacggcg cacggtgtct ggcggcaact
    16561 cgagcagccc cctgtccaga ttcttgcgaa tcaggtccgg gaacttgtcg tcttggttca
    16621 tggggatatc ccttggcgtt tgagcgcggc agccagcgtg tgggaggctc gcgagcagtg
    16681 cgtcttgacg ctgccctccg agcaacccat cgtcagtgcc gtctcagcga tatccaaatc
    16741 ctcccagtaa cgcagcagga aggcttctcg ttgacgcgct ggcaggcgac cgatctcgtc
    16801 ttcaatgata cgcatcagcc tgctttgcgc aaaggcgtcc tcggggccct gaagctggcc
    16861 cggcgcaccg gttgtatcga tgctttcgag cgggtcgcga gcgtcgtcgc catcgggaaa
    16921 cagactcgat agcagcgtgg tccaggtgtt gcggacacgg gtccgccggt agtggtcgcg
    16981 gatggcgttc tggaggatcc gctggaacag catcggcagt tcctcgaccg ggcggtcgcc
    17041 gtacttgtcg gagagccgca tcattgcgtc ctgaacgagg tcgagactgg cctcctcgtt
    17101 gcgggtcgca aacatggcgt gccggaacgc gcggcgctgg gtcgcggcga gaaactcgga
    17161 tagttcttgt cgggtagcca gggccttgat ttccttgtgg tcgggccgcc cgtaggtgcg
    17221 acgggcgcgc cttgaccgaa agggggcttc caattaaggt tgcgggtttt ccacgcaaca
    17281 gcaataaata cggggcaagg cgcgcaaatg atgctcaccg gtgcggaaat tctagtcagg
    17341 tgtctccagg aagaaaaggt cgaggtggtt ttcggctatc cgggtggggc agtcctgcac
    17401 atctatgacg aactgttcaa gcaggaccat accaagcatg tcctggttcg tcacgagcag
    17461 gcggccgttc atgcggccga cgcctattcg cgttcgtcgc atcgcgtcgg cgtcgcgctg
    17521 gttacttccg gaccgggtgt gaccaacgcc gtaaccggga ttgcgacggc ttacatggat
    17581 tcggtgccgg ttgtcgtgat taccggtcag gtgccgaccg cggcgatcgg ccaagacgcc
    17641 ttccaagaat gtgacacggt cggcattact cgcccctgcg tcaagcacaa tttcctggtg
    17701 aaggacgttc gtgatctcgc cctgacgatc aagaaggcct tccatattgc cagtaccgga
    17761 aggccggggc ctgtggtcgt cgacgtcccc aaggacatca cggcgcacaa atgccgcttc
    17821 gatggctatc cgcagaccgt gcagatgcgc tcgtacaatc cggtggtcaa gggccatctc
    17881 ggccagatca agaaggctgc gcagtacttg ctcgacgcca agcgtccgat gctctatttc
    17941 ggcggtggcg tcatcctgtc caacgcatcc gagcagctga cgaagctggc gcggcgcctg
    18001 aacattccgg ttaccggtac gttgatgggc cttggcggct atccgtcgac cgaccgccag
    18061 ttcctcggca tgccgggcat gcacggcacc tacgaagcga acatggcgat gcagcactcg
    18121 gacgtgctgc tggccgtcgg tgcgcgtttc gacgatcgcg tgatcggcaa tccggcgcac
    18181 ttcgccgaga acccgcgcaa gatcatccac atcgatatcg atccgtcgtc gatctcgaaa
    18241 cgggtcaagg tcgaggtccc catcgtcggc aacgttcccg atgtgctcga agaactcctc
    18301 aagctgctcg acgcatcgcc ggacaagcaa gcgccggccc atgaggactg gtggaaggaa
    18361 gtcgaagggt ggcgctcgcg caagtcgttg aactacaagc agaacggcaa cttgatcatg
    18421 ccgcagtacg tggtgcagaa gctctacgag atcaccaagg gcgatgcgat cgtcacctcc
    18481 gacgtcggcc agcatcagat gtgggcggcc cagtactacc agtttgacaa gccgcggcgc
    18541 tggctcaact ccggtggcct aggcacgatg ggcgtcggcc tgccgtacgc gatcggcgcg
    18601 acctttgcca atcccgacgc gacggtcgcc tgcgtgaccg gtgaagcctc ggtgcagatg
    18661 tgcatccaag agttatcgac gatcaagcag ttccgcctgc cgatcaagat catctgcctg
    18721 aacaatcgct atctgggcat ggtccgccag tggcaggaaa tgttctacgg caaccgctat
    18781 tcggagtcct atctcgattc gctgcccgac ttcgtcgcgc tagccgaggc atatgggcac
    18841 gtcggcatgc gcatcgaacg tcccgaagac gtcgaggagg cgctgcgcaa agccttcgtt
    18901 gaccacaagg atgattgcgt attcctcgac ttcatcaccg atcagacagc caacgtcttc
    18961 ccgatgatcg cggcaggcaa ggggctgacc gaaatgatcc tggccgaaga cctctgagag
    19021 cgagcacacg atgcgacaca tcatctccat actgattgaa aatgaatcgg gcgcgctctc
    19081 ccgggtggca ggcctcttct cggcacgtgg ctacaacatt gagacgctga ccgttgcgcc
    19141 gaccgaggat ccgtcgctgt cgcggatgac gatcctcacc gccggttccg acgacgtgct
    19201 cgagcagatc accaagcaac tcaacaagct ggtcgatatc gtcaaggttg tggacctttc
    19261 cgaggcggcg cacatcgaac gcgaactcat gctgatcaag gtccgcgcga ccggtaagga
    19321 tcgcgaagaa atgaagcgga tggccgagat attccgcggc cgcatcatcg acgtcaccga
    19381 gtcgacctac gtcatggaat tgaccggtcc gagcgaaaag ctcgatgcct tcatcggggc
    19441 gatcgacaag ggactgattc tggaaacggt gcggaccggt gtctgcggca ttggccgcgg
    19501 cgaccgcatc ctcaaggctt aagcgtcgaa agacggattc tcaattgaaa ggaaacacga
    19561 tgaaggttta ttacgacaag gatgccgatc tttcgctgat caagggacgc aaggtcacga
    19621 tcgtcggcta cggatcgcag ggccacgccc acgcccagaa cctgaaggat tccggcgtca
    19681 aggtcacggt tggcctgcgc aagggcggcg cctcttgggc gaaggccgag aaagccggac
    19741 tgaaggtcaa ggagatcgcc gacgcggtca agggcgccga cgtcgttatg atcctgctgc
    19801 ccgacgagaa catcccgtcg gtctactacg ccgaggtcga gcccaacatc aagaagggcg
    19861 ccgcactcgc cttcgcgcat ggattcaacg tccattacaa ccaggtcgtc gcgcgtctcg
    19921 acctcgacgt gatcatggtt gcaccgaagg gtcccggcca cacggtgcgc tcggaatacc
    19981 tcaagggcgg cggcgttccc tcgctgattg ccgtgcatca ggatgtatcg ggcaaggctc
    20041 gcgacatcgc tctctcctat gcggcggcca acggcggcac caagggcggc gtgatcgaga
    20101 ctaatttccg cgaggaaacc gagaccgatc ttttcggcga gcaggccgtg ctctgcggcg
    20161 gtgccgtcga actggtcaag atgggcttcg aaacgctgac cgaagccggc tacgcgccgg
    20221 agatggccta ctttgaatgc ctgcacgaac tcaagctgat cgtcgacctg atgtacgagg
    20281 gcggtattgc caacatgaac tactcgatct cgaacaatgc cgagtacggt gagtacgtca
    20341 ctggccccga ggtcatcaac gagcagtcac gggaggcgat gcgcaatgcg ctgaaacgca
    20401 tccagaccgg cgagtacgcc aagatgttta tccttgaagg acggacgaat tacccggcga
    20461 tgaccgcccg gcggcgtctc atggccgcgc atccgatcga gcaggtcggc gggcaactgc
    20521 gtgagatgat gccgtggatc aagaagaaca agctggtcga tcagtcgaag aactgaaaga
    20581 cgctgcttga aacggaggcg cgaatccggc ccggtgccga attcgcgctt cagtattgtt
    20641 gcctctgaca agcgagaacc gcgttgaact atccgcatcc catcatcgcc cgcgaaggct
    20701 ggccttttct ggcgatcgcc ctgattgctg ccatcgtggc gacctatgcg ggcggctggc
    20761 tgctcgcttc gccggtctgg atcattgcgc tgttcgtgtt gcagttcttc cgcgacccgg
    20821 ctcgcgaaat cgccggcggt ccgacttcgg tcgtcgctcc cgctgacggc cgaatcgtcg
    20881 ccgtcgagac cgtcgaagat ccctaccttg gccggcaggc gctcaaggtc agcgtgttca
    20941 tgaatgtctt caatgtgcac tcgaatcgca gtcccgttga cggccttgtc gtcggtcgct
    21001 ggtacgcggc cggaagtttt ctcaatgcgg cgctcgacaa ggcctcggtc gagaacgagc
    21061 gctgcgcgct gcacctgcgc actgcggccg gtctcgatgt cacctgtgtg cagatcgccg
    21121 gcctggttgc ccggcgaatc ctcaactatt cacgcgaagg tgactcgctg gcgcgcggcg
    21181 agcgattcgg attcatccgc ttcggttcgc gcgtcgatct gtatctgccg ctgcagactt
    21241 cggtaaaggt ggcaatcggg gagaaggtgt atgcttcttc gaccgttctc gccgagctcc
    21301 ccgcgcctgc cgcaacgaat gcctgaggaa ccgaattccc gcaaggcgct cttcaatccc
    21361 gaggtgcgtc gcaagggaat ctacctgctg cccaacctgc tgacgaccgg agcgctgttc
    21421 tccggttttt ttgccatcgt cagggcgatg cagggtgatt tcgaagctgc cgcaatggcg
    21481 atattcgtcg ccatggtgct cgacggactc gatggccgcg tcgcccgtct gacgcgaacc
    21541 cagagcgcct tcggtgccga gtacgactcg ctttccgata tggtttcctt tggtgcggcg
    21601 ccggcgctgg tcatgtacga atgggccttg cgcgacctcg gacgcatcgg ctggatcgcc
    21661 gccttcatct actgcgcgtg tgccgccctg cgactggcgc gcttcaacac gacgctcgag
    21721 gtaaccgaca agcgtttctt ccagggcctg ccgagtcctg cggcagcagc actggtggcc
    21781 ggattggtct gggtgatgat cgacaacggc atatccggca gcgatattcc gtggctggcc
    21841 tgcgtcctca ccgtctttgc cggcgtgacg atggtgtcca acatccgtta ctacagcttt
    21901 aaggacgtca acctgcgccg cagcgtcccc ttcatcgtcg tcgccggcgt ggcgctggct
    21961 tttgccctga ccgcgctcaa acccgacgct accttgttcg cggtctttgt cacctatgcg
    22021 ttgtccggct acgttctcgc gatttggcag cgattccgtc cggcgaagga cgttggcagc
    22081 agcgactccg ggagcgctta ggcgcagcct gtcgccagga ccgaatcagg ctgttgcgcc
    22141 ggccacgcgc cgacggtata ttgcggccca tgtctcgcgc aatttcatat tcgccccttc
    22201 tccttgcacg gctgctgctt gcacgtccgc tgctgcgctt cgcgcgctaa tccgaaaccg
    22261 acaattcaag cagtatttgt ggtgcgcgcc ggctgaggtg cgcgccgagt cagtacatct
    22321 gaagcctgga gacgaacatg aaagaccgat tgattatttt tgacacgacc ttgcgcgacg
    22381 gcgagcaaag tccgggcgca tcgatgaccc gcgacgagaa gttgcgggtg gcccggcagc
    22441 tcgaacggat gaaggtcgac gtcatcgagg ccggatttgc cgcggcttcc cctggggatt
    22501 tcgatgcgat ccgggcaatc gctgaagccg tcaaggattc gaccgtttgc tcgctggccc
    22561 gcgccaacga gaacgacatc cgccgcgctg gcgaggcgat ccggccggcg cgttcgaagc
    22621 ggattcacac cttcatcgcg accagcccga tccacatgga gaagaagctg cggatgacgc
    22681 ccgaacaggt cgtcgaagcg gcgattcggg cagttcgcct ggccggcgag tacaccgacg
    22741 atatcgaatt ctcagccgag gatgcggttc gctcggacat ggatttcctc tgccgcgtct
    22801 ttcaggcggt gatcgaggct ggcgcgacga cgatcaacgt gcccgatacg gtcggctaca
    22861 gcgtcccctc ggtctggggc gagcggatgc gcgaactgat cgagcgggtg ccgaattccg
    22921 ggagggtggt ctggtcgacc cactgccata acgacctcgg catggcggtt gcgaattcgc
    22981 tctcggcggt tctggccggc gcgcggcagg tcgaatgcac ggtcaacggc ctaggcgaac
    23041 gcgccggcaa cgcctcgctt gaggaggtgg taatggcggt gcgtacccgc agcgacatct
    23101 tcccggtcga gaccggagtc gatacctcgc agatcgtacc ggcgtccaag ctggtttcgc
    23161 agatcaccgg ttatccggta cagccgaaca aggccatcgt cggcgccaac gcctttgcgc
    23221 atgagtcggg aatccatcag gacggcgtgc tcaagcaccg cgagacctac gagatcatgc
    23281 gtgccgaaga cgttggctgg tcacagaaca agctcgttct tggcaagcat tcggggcgca
    23341 acgccttcaa gacgcggctg aatgatctcg gggtcgtcct tgccagcgac gaggcgctga
    23401 attcggcttt cgcccgcttc aaggaactcg ccgaccgaaa gcatgagatc ttcgacgagg
    23461 acctgcaggc gctggtctcg gacgagcgcg tgacgcccga ggacgagcac ttcaagctca
    23521 tctattcgcg agtctgttca gagaccggcg agatgcccga gtcgaatctc accatgtcgg
    23581 tcggcggcgt cgaaaagcgg gcgactgcca gcggcggcgg accggtcgat gcgaccttcc
    23641 gtgcaatcga ggccgtcgtc ggcagccagt ccgaactgct cctgtattcg gttaacgcga
    23701 tcacgacggg caccgatgcg cagggcgagg tgaccgtccg cctggcccgc ggcgggcgca
    23761 tcgtcaacgg ccagggcgcc gataccgaca tcgtcgtggc ctcggccaag gcgtatctca
    23821 acgccttgaa caagctcgaa attcatcccg aaaagctcaa cccgcagggg gatgtctagg
    23881 tttcgggcct gtctgacgcg gatcggcgcc ccctcggggg ccgtggtccg cggctggcgc
    23941 gaatgtgagc gaccgcggtg atgaaaaaga gcaggctcaa cagcagttcg attgccgcga
    24001 gcacccgcgt cagcccggta tcactcatca gccggaccgt gccttccagg acgaagagca
    24061 ggatcagcat cgatgcccac tggtacgtat acacgcgtcc cgcgatgacc cccgccagcg
    24121 gcagggcaag caccagcgct ttcagtacca gccaggagcc gccgggccgc agcggggcga
    24181 gccatagttc ccaaagcagc gcaagcgcga tcagggcgca caggcagagc gttgcccagg
    24241 cgcggaacgg gctcggtctt gggttgctgc tgggcagctc ggcggaagcg gacatgggaa
    24301 ttttgctggc ggacaggcgc attctagccc gcggtatcgc ggagaccggg aagtgatcga
    24361 ctacttctct cgcctgttcc gccgtttccg cgaagagcgt ttcgcccaac tcgctgccag
    24421 tctatcgttc acgaccttgc tgtcgctggt gccgctcgtt gccgtggtca tcatcatcgg
    24481 caaccgcctg ccctggatcg gcggcctggt cggctacctc gacaacttcg tcatcgagaa
    24541 cctgttgccg gcacagaccg gcgatgtcgt tgcgcgctac gcctaccggt tctcgcggca
    24601 ggcgaaccgc ctgactttcg gcggcgcggt gctgctgctg gtggcatcga cggcgctgat
    24661 gttctcgatc gagcgtgcct tcaatcacct atggcgagtc cggcagccgc gatcgctggc
    24721 tgaaagaatc tttctctaca ccatagcaat cgccgttttc ccgctgctcg ccggcgggct
    24781 gttggggatc agcgcctttc tcgtgaacgc ggcgatgcca ttggccggcc agcgtgagtg
    24841 ggcccgctac ctggccgtcc acggcagcgc cggcatgctg ctctggggat tgttcgcggt
    24901 gctctacgcg tcggtaccgg catgccaggt acgctggcgc cacgccttga ccggcggtgc
    24961 cgttgccgcg atcggcgtga cggctgccca gcgcctgttc ggctacgcta tcgaccaagc
    25021 gagcgtctac aagacactct acggcgcgtt ttcggtcctg ccgatctttc tggtctggat
    25081 atacctgtgc tgggcgatcg ttctggtcgg cgcactggtc gccgccaatt tcgccggacc
    25141 tggcccgctc aggcggcgct agctagcgtc agggtgacgc tcagcccgcg ctgccgctgg
    25201 cgatcaggta gaggtcggtg ccggccgtgc tctcgacgcg ccgttcgaga cggatctcgc
    25261 ggatgccgac gttgggccct tcggtgatga tcacgtggct cgtctgggga tcgagtacaa
    25321 ggccggcggg aaccgccagc gtgctgttcc cctggccggc gatcggcgcc ataactagaa
    25381 ccggctgctt gctgatttcc tccgggcccg gcagcgccag catgccgccg acggcacgtg
    25441 gtgcgagagc ctgcagaccg agctcgaagt gctccggatt ctcgctttgc gcccagcgga
    25501 tgatgcagat ctgccagtct tcgtcgtgat aacgccgaat tgccgccaga tccccgacct
    25561 gaagcttctg cgtcttgccg gtgacgtgca tcgttgcgta accgtccggg ctctcgttga
    25621 cgacctgcca ttcgccgagc tcgtcgtcat cccgttggcc tcggagtaat tgccagatgg
    25681 catcgaggcg gaagcagatc cgcgcccggt agccttgccg gcggcgcgaa aaccgtcgtt
    25741 tgcgggtgtc cgacagcaga ttgcgcaggc gctgcagcgc ggccagcgcg gcgcccatcg
    25801 gcaggtcgtc ggcgagcagc gccagcgcac gacgagactc ctcgtcggaa ctggccagcc
    25861 gatcattgat ggcagcgcca atgctggcga catcgaagta aaggcagtcg ctgccgattg
    25921 gcggcggttt gcgcgcgaac gccaccggcg gcatgtcgtt cttcaggttg acccagaact
    25981 gcccttcctc cctactaggc ggctggttgc caagtgaaat cgtcgacgcg aggtcggcca
    26041 gatagcggtc gatgaaacac cattcggcac tggtgaaggt cgccgggtgg gggcagccag
    26101 ccagcaaaaa gcgcgcgtac tgggtgccga tggaagcgct gcgctcgggc gggatcgcgt
    26161 cgaggaggtt catcgagcta gccttggccc ggatgtcgtg agcaatccgc cagatctccg
    26221 gtccatgcgg cgccgccgcg agattggaca ggagctggtg tttgcgcagg ctgtcgagtg
    26281 ccagccagag tgactgctcg ggcgggcgct gcagcccctt gatcagtcgt tcggtcgaac
    26341 cgatttcacc aaagtagaga tgcgccatgg tttccagcgc atcctgaagc cggcgtacca
    26401 gcgttctgac gcgaaccgac agcggcagac gggtcttccg cagattggcg aaagcctgtt
    26461 ctacgatgcg gtcggcgcgg tcctgtatct gcgtcgtgag gcgctgccgc agcgccgggg
    26521 aaaactcgtt ttcggcaacc gcgcgaacgt aggagcgcag gcggtcgata tcgtgatcga
    26581 cctcctcact ggggggcgtc gccagccacg ccaggaccgg ttgggcattg gcgtcggcgg
    26641 attgagcgga cctgtgctct ggcagagcca tcgaaaagat tacaacggga tacggaaccg
    26701 cggaactata gcagacgggc gcgggaggcg gttgccgcgg ccaaatcagg gatagcagcc
    26761 ggccattgcc ggactggtgt taaccggtat aatccgcccc ttttctcgct agcttggata
    26821 cagacaaatg gtagttattc gtctttcgcg cggtggcgcg aagaagcgac ccttctacag
    26881 catgatcgtg accgactcac gcaatcgccg tgatggccgg ttcgtcgagc gcatcggctt
    26941 ctacaatccg atggctgccg aaaacgaact gggtctcaac gtcaacgtcg gtcgcctcga
    27001 gttctggaag ggacagggcg ctcagctgtc gccgaccgtc gctcgcctgg tcaagcagtt
    27061 caaaccccag caggctgcct gagtcagtta gcggcagcag cttcggcgct gctgcgttgg
    27121 cggatgtcgt gagcagcagc gaagcgggtt tgccggtcgg cgccaatgcc ggcccctacg
    27181 tcgtgctggg tcgcgttagt ggcgcctacg gtgtgcgcgg ctggctgcga attcaggttt
    27241 ttggcgacga tccgctttcg tggcgcgaga tggacacttg gtggctgtcg cggtcccgcg
    27301 accgcttaac gaatgatccg tccagcgacg aagactggga ggcctacggg ctcggtcaat
    27361 gtcgcctgca gggtggtgat ctcgttgcac agctgactgg aatcgccgat cgagacgcgg
    27421 cagaggcgct gaagggcgcc ctgctcgctg tgccgcgggc gcatttgccc gatcccggga
    27481 aggacgccta ctactggggc gacctgcttg gcatggcggt ggtcggaacc ggcaacgttc
    27541 cattgggggt cgtcgtccgc ctgctggaaa ccggcgccaa cgacgtgttg gtggtgattg
    27601 acgcggatgg gcatgagcgt ctgctgccct tcgtcgagtc ggtgatcgcg cgggttgacc
    27661 ccgcggtgcg gcggatcgac gc
//
LOCUS       DATDAA010000033         2715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_142143,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000033 DATDAA010000000
VERSION     DATDAA010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142143"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VIS73_01295"
     CDS             complement(<1..339)
                     /locus_tag="VIS73_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY5761822.1"
                     /translation="MLLRLSVVAVLMFGFGFALVPFYEQICAALGVNSLVPIERAAAK
                     NTQIDRARTVSIEFDANSHDLPWRFRPLVRHLQVHPGELATIEYEVVNVRPHAVTGQA
                     VPSYGPSRAGE"
     gene            complement(520..2118)
                     /gene="ctaD"
                     /locus_tag="VIS73_01300"
     CDS             complement(520..2118)
                     /gene="ctaD"
                     /locus_tag="VIS73_01300"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY5761823.1"
                     /translation="MSAVVDSHGHGHEHHHDHHGGYSGIMRWITTTNHKDIGTLYLWF
                     SFIMFMVGGAMAMVIRLELFSPGLQWVDPDFFNQMTTMHGLIMVFGAIMPAFVGFANW
                     LIPMQIGAPDMAFARMNNWSFWILPFAASLLVISFFMPGGAPGVGWTLYAPLTVQMGV
                     GMDLTIFAVHLLGMSSIMGSINIITTILNLRAPGMTLMRMPLFCWTWLITAYLLVAAM
                     PVLAGAVTMTLTDRHFGTTFFNAAGGGDPVLYQHIFWFFGHPEVYIIALPAFGVISQV
                     IPAFSRKPLFGYASMVYATSAIAILSFIVWAHHMYTVGMPITGQLFFMYATTLIAVPT
                     GVKVFNWVATMWKGALSFETPMLFAIGFVFLFTLGGMTGLVLSLVAIDIQLHDTYYVV
                     AHFHYVMAIGAVFAGFAGVYFWLPKWIGRMYDETLGKWHFWLSTIFINVTFFVQHFSG
                     LAGMPRRIPDYPLMFETWNKISSIGAFGFGLSQLLFLYIVLKAIRSGEKAKDKQWEGA
                     DSLEWTHLPTPVPYHSFETQPVLK"
     gene            complement(2149..>2715)
                     /gene="coxB"
                     /locus_tag="VIS73_01305"
     CDS             complement(2149..>2715)
                     /gene="coxB"
                     /locus_tag="VIS73_01305"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY5761824.1"
                     /translation="LIILVIIAWPATKVVIAQKDTSQPDLTIKVTGYQWKWGYDYVKG
                     EGEGIRFVSTLATPRDQIEGRAPKGENYLLEVDNEMVVPVKKKVRVLTTAADVIHAWW
                     IPAFGAKQDAIPGFIRDIHFTAKQTGVFRGQCTELCGKDHGYMPIVVRVVSAEEYTKW
                     VGEQKAKATAAAEAPKKIAAAAAETPRQ"
BASE COUNT          523 a          866 c          852 g          474 t
ORIGIN      
        1 ttcgccggcc cgcgacgggc catagctcgg caccgcctgg cccgttaccg catgcgggcg
       61 tacgttgacc acctcgtact cgatggtggc gagctcgcct ggatgcacct gaaggtgtcg
      121 caccagcggc cggaagcgcc atggcaggtc gtgcgagttg gcgtcgaact cgatggagac
      181 ggtgcgtgcg cgatcgatct gcgtattttt cgccgctgcc cgctcgatcg gcaccagcga
      241 gttcaccccg agcgcagcgc agatctgctc gtagaacggc accagcgcga agccgaagcc
      301 gaacatcagc acggcgacga ccgacaggcg cagcagcatg cgccggttat cacgctggcg
      361 cggatcgacg gttaccggct tcggctcagt ttcgtgcatg acgtgccgcg gatcagctgc
      421 cgcggaggat ccagaacttg acaatgatcc cgatgaacga aatcagcgag atcgtcgcca
      481 gcgccagcgc ggttttcaga ttgcggctca ccctgccgcc tacttcagca ccggctgggt
      541 ttcaaacgag tggtacggta ccggcgtcgg caggtgcgtc cactcaagcg agtcggcccc
      601 ctcccattgc ttgtccttcg ccttctcgcc actgcggatg gccttcagta cgatgtacag
      661 gaacagcagt tgcgacagac cgaagccaaa ggcgccgatc gagctgatct tgttccaggt
      721 ctcgaacatc agcggatagt ccgggatgcg gcgcggcatg ccggccaggc cggagaagtg
      781 ctgcacgaag aaggtcacgt tgatgaagat cgtcgacagc cagaagtgcc acttgccgag
      841 cgtctcgtcg tacatgcgcc cgatccactt cggcagccag aagtagacgc cggcaaaccc
      901 ggcgaacacc gcgccgatcg ccatcacgta gtggaagtgc gcgaccacgt agtaggtgtc
      961 gtgcagctgg atgtcgatcg cgacgagcga gagcacgagc cctgtcatcc cgcccagcgt
     1021 gaacaggaac acgaagccaa tcgcgaacag cattggcgtc tcgaacgaga gcgccccttt
     1081 ccacatcgtc gcgacccagt tgaacacctt gacgcctgtg ggcaccgcga taagcgtggt
     1141 cgcgtacatg aagaacagct gcccggtgat cggcatgccg accgtgtaca tgtggtgcgc
     1201 ccagacgatg aacgacagga tcgcgatcgc cgacgtcgcg tacaccatcg acgcgtagcc
     1261 gaacaggggc ttgcgcgaga acgccgggat cacctgcgag attacgccga aggccggcag
     1321 cgcgatgatg tacacctcgg ggtggccaaa gaaccagaag atgtgctggt agagcaccgg
     1381 gtcgccgccg cccgcggcgt tgaaaaaggt cgtgccgaag tggcggtcag tgagcgtcat
     1441 cgtcaccgcg ccggcgagca ccggcatcgc cgcgaccagc aggtaggcgg tgatcagcca
     1501 tgtccagcag aacagcggca tgcgcatcag cgtcatgccc ggcgcgcgca ggttgaggat
     1561 cgtggtgatg atgttgatcg accccatgat cgagctcatt ccgagcaggt gcacggcgaa
     1621 gatcgtcagg tccatgccga cgcccatctg caccgtgagc ggcgcgtaca gcgtccagcc
     1681 cacgcccggc gcaccgcccg gcatgaagaa cgagatcacc agcagcgacg cggcgaaggg
     1741 caggatccag aacgaccagt tgttcatgcg cgcgaacgcc atgtcgggcg cgccgatttg
     1801 catcgggatc agccagttcg cgaagccgac aaatgcgggc atgatcgcgc cgaaaaccat
     1861 gattaggccg tgcatggtgg tcatctggtt gaagaagtcc ggatcgaccc attgcagccc
     1921 cggcgaaaaa agctcgaggc ggatcaccat cgccatcgcg ccgccgacca taaacatgat
     1981 gaagctgaac cacaggtaca gcgtgccgat gtccttgtgg ttggtcgttg tgatccagcg
     2041 catgatgccg ctgtatccgc cgtgatggtc gtggtggtgc tcgtgcccgt gaccgtggct
     2101 atccacaact gcgctcattc tcgatctcct aaacgctgaa ttcggtgctt actgccgcgg
     2161 cgtctcggcc gccgccgccg caatcttctt cggcgcttcg gctgccgcgg tggccttcgc
     2221 cttttgctca ccgacccact tcgtgtactc ctcggcgctc acgacgcgca cgacgatcgg
     2281 catgtagcca tggtccttgc cgcacagctc ggtgcactgc ccgcggaaca cgccggtctg
     2341 cttcgcggtg aagtgaatgt cgcggataaa ccccggaatc gcgtcctgct tggcgccgaa
     2401 ggcagggatc caccaggcgt ggatcacgtc ggcggcggtc gtgaggacgc gcaccttctt
     2461 tttgaccggc accaccatct cgttgtcgac ctcgagcagg tagttctcgc ccttcggcgc
     2521 gcgtccctcg atctggtcgc gcggcgtcgc cagcgtggaa acgaagcgaa tgccctcgcc
     2581 ctcgcccttc acgtagtcgt agccccactt ccactggtag cccgtgacct tgatcgtgag
     2641 atccggctgc gaggtgtcct tctgcgcgat gacgaccttg gtcgccggcc aggcgatgat
     2701 gaccaggatg atgag
//
LOCUS       DATDAA010000034         2470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_72393,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000034 DATDAA010000000
VERSION     DATDAA010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72393"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VIS73_01310"
     CDS             <1..431
                     /locus_tag="VIS73_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5761825.1"
                     /translation="AMNARAAGIFRHGVYGNTMTTNPRALEVAVSVLDALTPELRENI
                     RRRGAELVERLQALAGELGDAITGVQGTGLLFSCELNERYKCFGADSTEDYLRRQGLG
                     VIHGGARSLRYTPRFAIGADEVDLIIDLTRKALLDGPIHG"
     gene            complement(437..946)
                     /locus_tag="VIS73_01315"
     CDS             complement(437..946)
                     /locus_tag="VIS73_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEY5761826.1"
                     /translation="MANESRAGFLSLLDMRPGTELGASDWYAVTQEDIDAFARLTRDE
                     DPYHTDPGWAREHSPLETTISFGFLTMSMLTHFLHQVFGRLGIESGDDIQVFNFGFNR
                     VRMPEPVPAGAEIRGRFSFVGARLRDNGGAEITVNAVVDIKGNDRPALVAEWLFVAVR
                     GDGQGIDSC"
     gene            1195..1929
                     /locus_tag="VIS73_01320"
     CDS             1195..1929
                     /locus_tag="VIS73_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761827.1"
                     /translation="MIINRLSALIRRQDWSSLTIEMIVLVLGIFAGLQVNNWNEDRLE
                     AKRLDRQLVSLAEEMKENLKRLDAYQDQLTGIIEDMERFRRLFLENPGEVSADELDRM
                     LWRSIPIPTYQAKRFVLDAVLTSENFSARQVGQLAGLLQAWDELQRRLARVDQDALVY
                     RDEHIHGIYTRRIAFSSIERYMEPPAQGLPASPFGNRPEEILADRVIENIATVRLTMT
                     LGNLEYVEDLRDKTAEIVAFIENPPR"
     gene            complement(1948..2397)
                     /locus_tag="VIS73_01325"
     CDS             complement(1948..2397)
                     /locus_tag="VIS73_01325"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY5761828.1"
                     /translation="MNSRSSGGCMCGSVRYETTGEPFAINYCHCQSCRRHNGGPVVAL
                     AGYQAEQVQFSGAERKKYESSPGALRAFCENCGTPLTWEGDGGELGQIVEIHISTFDE
                     PESMVPTSHAFEPERLAWFDVADDLPRFEGFSDMSPLLHHGPAPEGD"
BASE COUNT          471 a          775 c          767 g          457 t
ORIGIN      
        1 tcgcgatgaa cgcgcgggcg gccggcattt tccgtcacgg cgtgtacggc aacaccatga
       61 cgacgaaccc gcgcgcgctc gaggtggccg tctcggtgct ggacgccctg acgccggaac
      121 tgcgggagaa catccggcgg cgcggcgcgg aactggtcga acgattgcag gcgctcgccg
      181 gggaactcgg cgacgcgata accggcgtcc aggggacggg tctcctgttc agctgcgagc
      241 tcaacgagcg ctacaagtgc ttcggcgccg acagcaccga ggattacctg cgccgccagg
      301 ggctcggcgt gatccacggc ggcgcacggt cgctccgtta cacgccccga ttcgcgatcg
      361 gcgcggacga ggtcgatctc atcatcgatt tgacccgcaa agcgctgctc gacggcccga
      421 tccacggctg aatcggtcag caggaatcaa tcccttgtcc gtcgcccctg acggcgacga
      481 acagccattc ggcgacgagc gcggggcggt cgttgccctt gatgtcgacg actgcgttca
      541 cggtgatctc ggcgcctccg ttgtcgcgca gccttgcgcc gacaaaactg aatcgtccgc
      601 ggatctcggc gccggccggg acgggctccg gcatccgcac ccggttgaag ccgaaattga
      661 acacctggat gtcgtcgcca gactcgatgc ccagccgccc gaagacctgg tgcaggaaat
      721 gggtcagcat ggacatggtc aggaagccga aagatatcgt cgtctcgagg ggcgagtgtt
      781 cccgggccca cccggggtcc gtgtgatagg ggtcctcgtc ccgggtgagc ctggcgaagg
      841 catcgatgtc ttcctgggtc accgcgtacc agtccgacgc gcccagttcc gtgcccggcc
      901 gcatgtccag caacgataag aaacctgccc ggctttcgtt cgccattgaa tgcgcccccg
      961 gttcttgcaa tggactcgag cgttgacagt aaataaaggc gcgcccttcg gcaaatcggg
     1021 gacgctggat gaattcctga atgaccggca gcataattgc ctggcagtcg aagcccgggt
     1081 caccggggct gccggctcct ctgccggacc gaccgcaatt tcgctcgaaa ccgcgttccg
     1141 gcaccatggc cggttcaagg gcattcgttg ctggagctga aaaggggata gcggatgatt
     1201 atcaaccgac tctcggcgct gatacgccgg caagactggt catcgttgac catcgaaatg
     1261 atcgtcctgg tgctcggtat tttcgccggc ttgcaggtca acaactggaa tgaggaccgc
     1321 ctcgaggcga agcgactgga tcggcagctt gtctcgctgg cggaggagat gaaagaaaac
     1381 ctcaaacgcc tcgatgccta tcaggaccag ctgaccggga tcatcgaaga catggagaga
     1441 tttcgacgtc tctttttgga aaacccgggt gaagtctccg ccgatgaact cgacaggatg
     1501 ctctggaggt ccataccaat accgacctac caggccaagc gtttcgtcct tgatgccgtg
     1561 ctgacgtcgg agaatttctc ggcaaggcag gtcggccagc ttgccggcct gctgcaagcg
     1621 tgggacgaac tccagaggcg actggcccgg gtcgaccagg atgccctcgt ttaccgggac
     1681 gagcacatcc acggtattta tacccggagg atcgcctttt ccagcatcga gcgttacatg
     1741 gagcccccgg cacagggtct tcccgcatcg cccttcggca atcggccgga ggaaatcctc
     1801 gcggaccggg tcatcgagaa catcgccact gtccgcctga cgatgacttt gggtaatctg
     1861 gagtacgtcg aggatttgcg tgacaagact gcggagatcg ttgcgtttat cgaaaacccg
     1921 ccgcggtgac ccaggcgcag gcgcggctca atcgccctcc ggcgccggcc cgtgatggag
     1981 cagcgggctc atgtccgaaa atccctcgaa acgcggcaga tcgtcggcca cgtcgaacca
     2041 tgccagtcgc tcgggctcga aagcgtgcga tgtcggcacc atgctttccg gctcgtcgaa
     2101 ggtgctgatg tggatctcga cgatctggcc aagctcgccg ccgtcgccct cccacgtaag
     2161 cggcgtcccg cagttttcac aaaaggcccg caacgcgccg ggcgaggatt cgtatttctt
     2221 cctctcggcg ccgctgaact gcacctgctc ggcctggtag cctgcaagcg cgaccaccgg
     2281 accgccattg tgcctgcggc aactctggca gtggcagtaa ttgatcgcaa agggttcgcc
     2341 ggtcgtttcg tatcgaaccg atccacacat gcaaccgccg gatgacctgc tgttcataac
     2401 gcgctctcct gtcgggggtc gatatcaagg ttgaaatcaa agagggcctt gcgtccgacc
     2461 tgactgcctt
//
LOCUS       DATDAA010000035         2015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_456835,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000035 DATDAA010000000
VERSION     DATDAA010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456835"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VIS73_01330"
     CDS             complement(<1..275)
                     /locus_tag="VIS73_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761829.1"
                     /translation="MTHATRKLAVGLAASLALTVTAAMAQDHSHHQHHQTDTTPKAAA
                     ASEVKYADTVLIDQFGQKRKLASEVFADRIVVLDFAYTTCTTICPVLT"
     gene            complement(362..>2015)
                     /locus_tag="VIS73_01335"
     CDS             complement(362..>2015)
                     /locus_tag="VIS73_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HEY5761830.1"
                     /translation="AVQKQCVEKIGLYLKGAVGIRPLVDLNGYYLLVMNKDPSITVLD
                     PMVSMAGRTSTLTTIKLKSSPMDWVNHDAQKLLYVSMPAVGELAVVDTETFKVTREVA
                     VGEAPTRTLLQPDGRYLWIGNNARDPKKSGVTVVDTKGMKVVLQAATGRGHHEIAISS
                     DNRLAFVTNRDDNTVTVFDVGTLKQIKQIKTGELPLSIAYSALSRAAYVANGKDGTIT
                     VIDAGKLEVVKTIKAKPGLGPLRMTPDGRFAMVVNTMEGVVNVIDPGTNEIIQSPQVK
                     EEPFQIAFTRGFAYVRSLGSERVSMINLASLGQGKEAVVQSFAAGSYTPKMGGDLPLA
                     DTVVGGLGEAAAFVVSPADNTTYFYMEGMNAPMSSYPSRGSYARAVTVVDRSLREIEP
                     GVFAGRFRMPVAGHFDVALSLDQPSMVHCFSTDAKADPQLEAARKVVEVEFLPQQRIF
                     RPQETGTVRFRILEGTGVPKTGLRDVLVRSFMVPASPPSDVVAKEVGDGIYEARIALP
                     ETGAYYVYISVPSLKLGYSDGAYLSLMAKGAPAAPASTSEKN"
BASE COUNT          337 a          618 c          672 g          388 t
ORIGIN      
        1 gtcagcaccg ggcagatcgt cgtgcaggtg gtgtaggcga aatcgaggac gacgatgcgg
       61 tcggcaaata cctcgctggc cagcttgcgc ttctggccga attggtcgat cagcaccgta
      121 tccgcgtact tcacttcgga cgcggccgcc gccttcggcg tagtgtcggt ctgatggtgc
      181 tggtggtggg aatgatcctg ggccatcgcg gccgtcaccg tcagggcgag cgacgcggcg
      241 aggccgacgg cgagcttgcg ggtagcgtga gtcatgggtg tccttctgtc atcaatcggt
      301 ggggccaaag cggcgccgtt gcgggcgccg acgacctagc gacgctacac gccgcttcct
      361 cttaattctt ctcgctcgtg ctcgccgggg ctgcaggcgc ccctttggcc atcaggctca
      421 ggtaggcgcc gtcgctgtaa cctagcttga gcgagggtac gctgatgtag acgtagtagg
      481 ccccggtttc aggcagcgcg atcctggctt cgtagatgcc gtcgccgact tccttggcaa
      541 cgacatcgga aggcggcgag gccggcacca tgaatgagcg gactaacacg tcacggaggc
      601 ctgtcttcgg caccccggtg ccctcgagga tgcggaaacg cacggtgccg gtttcctgcg
      661 ggcggaagat ccgctgctgc ggcaggaact cgacctcgac aaccttgcgc gccgcctcga
      721 gctggggatc tgccttggcg tcggtcgaga agcagtgaac catgctcggc tggtcgagtg
      781 acagcgcgac atcgaagtga ccggcgacag gcatgcggaa gcggccggcg aagacgccgg
      841 gctcgatttc gcgcaggctg cgatcgacga cggtcaccgc gcgcgcgtag ctgcctcgac
      901 ttggatagct cgacatcggc gcgttcatgc cttccatgta gaaataggtc gtgttgtcag
      961 cagggctgac aacgaaggcg gcggcctcgc cgaggccgcc aacaacagta tcggcgagcg
     1021 gcagatcgcc acccatcttc ggcgtatagc tgccggcagc gaagctctgc accacggcct
     1081 ccttgccctg accgagcgag gcgaggttga tcatcgacac gcgttcgctg ccgaggctgc
     1141 gcacgtaggc gaagcctcgc gtgaaggcga tctggaaggg ctcttccttg acctgcggcg
     1201 actggatgat ttcgttggtg cccggatcga tgacgttgac cacgccctcc atggtgttga
     1261 cgaccatcgc gaaacgcccg tccggagtca tgcgcaaggg accgaggccg ggcttggcct
     1321 tgatcgtctt gaccacctca agcttgccgg cgtcgataac ggtgatcgtg ccgtccttgc
     1381 cgttggccac ataggcggct cgcgagagcg ccgaataggc gatcgacagc ggcagttcgc
     1441 cggtcttgat ctgcttgatc tgcttcagcg tcccgacgtc gaatacggtg accgtgttgt
     1501 cgtcacggtt ggtaacgaag gcgagccggt tgtccgagga aatggcgatc tcgtggtgcc
     1561 cccgacccgt ggcggcctgc agcaccacct tcatgccctt ggtgtcgacg acggtgacgc
     1621 cgctcttctt cggatcacgc gcgttgttgc caatccacag ataacgaccg tcaggctgca
     1681 gcagggtccg cgtcggcgcc tcgccgacgg cgacctcgcg ggtgaccttg aaggtctcgg
     1741 tatcgacgac ggcgagttcg ccgaccgccg gcatactgac gtagagcagc ttctgcgcgt
     1801 catggttgac ccagtccatc ggcgaggact tgagcttgat ggtggtcagc gtgctggtcc
     1861 gccccgccat cgacaccatc gggtcgagca cggtgatgct cgggtccttg ttcatcacca
     1921 gcaggtagta gccgtttagg tccaccagcg ggcggatacc gacggcgccc ttgagataga
     1981 ggccgatctt ttcgacgcat tgtttctgca cggcg
//
LOCUS       DATDAA010000036         3039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_456851,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000036 DATDAA010000000
VERSION     DATDAA010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456851"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /gene="panD"
                     /locus_tag="VIS73_01340"
     CDS             complement(<1..303)
                     /gene="panD"
                     /locus_tag="VIS73_01340"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004990637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEY5761831.1"
                     /translation="MQRTLLKSKLHRLTATHADLHYEGSCAIDQDLLDAADIQEYEQI
                     DIWNINNGERFTTYAISAERASGIISINGSAARKAAPGDLLIIATFASYDEKEAGAH"
     gene            complement(316..1146)
                     /gene="panC"
                     /locus_tag="VIS73_01345"
     CDS             complement(316..1146)
                     /gene="panC"
                     /locus_tag="VIS73_01345"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY5761832.1"
                     /translation="MDVLDTVAAVRGTLYGKGTIAFVPTMGNLHAGHIALMERARALA
                     DTVVSSVFVNRLQFGPNEDFDRYPRTFAADCEQMAAAGVDFVFAPGEADLYPSPQVFS
                     VALPPIENELEGAVRPGHFRGVATVVLKLFNIVQPQIAVFGQKDYQQLMIIRQMVEQF
                     ALPIEIVVGPTVRADDGLALSSRNGYLSPAERAEAPRLNATLRHVRERLLTGETTDIG
                     ALEREAVATLNRHGWQADYVAVRRRSDLQSAPPDYAGPLVVLGAARIGSTRLIDNIEV
                     "
     gene            complement(1159..1974)
                     /gene="panB"
                     /locus_tag="VIS73_01350"
     CDS             complement(1159..1974)
                     /gene="panB"
                     /locus_tag="VIS73_01350"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY5761833.1"
                     /translation="MSVQPAQARRLTTHDLFRMRREGQRIAMLTAYDATFAALLEAAD
                     VDTILVGDSLGMVLQGHDSTLPVSLDDMAYHVACVTRGCRRPLIIADMPFGTFQVSAK
                     ESFRAAARLMAAGAQMVKIEGGNEMVETVNFLTTRGIPVCAHIGLTPQSVHQLGGYRV
                     QGKDEASAARLRTDAAALEAAGASLVLMEAVPAKLAAEITTALHVPTIGIGAGSATSG
                     QVLVLHDMLDLTAGKKPRFVRNFMAGASSVQDAVAAYVAAVRDGSFPAAEHTY"
     gene            complement(1980..2642)
                     /locus_tag="VIS73_01355"
     CDS             complement(1980..2642)
                     /locus_tag="VIS73_01355"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEY5761834.1"
                     /translation="MTDSRVRNVLAEARHIVVEGPIGAGKTSLAMRLAAHLEAGMLLE
                     QPGANPFLARFYQDPKRYALQTQLFFLFQRVDQLRDLSQPDIFGSRVVADYLLDKDPL
                     FAQLNLADDEYALYRQIYGTLAPQAPAPDLVVYLHARPETLIARIRKRGIESERRIGE
                     GYLALLAERYASFFYQYDEAPVMIVNSENLNFVDDDNDFVLLVERLSQMRGPREYLNR
                     GE"
     gene            complement(2647..>3039)
                     /locus_tag="VIS73_01360"
     CDS             complement(2647..>3039)
                     /locus_tag="VIS73_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HEY5761835.1"
                     /translation="AALKSFTELAKMDHLIYKFEIYKVLTGVSAKRAEEFASHTACRL
                     GKWYYEGEGKQCFADLDGYRAMEKPHGEVHQFGREAVNLHHKGEMIAATAQLARMEAA
                     SAEVLSCLERMSVHGQNSPDILCLDHAA"
BASE COUNT          509 a          911 c         1052 g          567 t
ORIGIN      
        1 gtgtgccccg gcttctttct cgtcgtagct cgcgaaggtg gcgatgatca acagatcccc
       61 tggggcagcc ttacgtgccg ccgaaccgtt gatcgaaatg atgccggagg cgcgctcggc
      121 ggaaatcgcg taggtcgtga agcgctcgcc attgttgatg ttccagatgt cgatctgctc
      181 gtattcctgg atgtcggccg cgtcgagcag gtcctgatca atcgcgcagg aaccttcgta
      241 gtgcaggtcg gcgtgggtcg cggtgaggcg gtgcagcttg gacttgagga gggtgcgttg
      301 cattggggga ctccttcaga cttctatgtt gtcgataagg cgggtgctgc caattcgcgc
      361 ggcgccgagt acgaccagcg gcccggcgta gtcggggggc gctgattgta agtcggatcg
      421 ccggcgcacg gcaacgtagt cggcctgcca gccgtgccgg ttcaaggttg ccacggcctc
      481 gcgctcgagt gccccgatat ccgtagtttc gccggtgagc aagcgctcgc gtacgtgacg
      541 cagcgtcgcg ttgaggcgcg gcgcttcggc gcgctcggcc ggcgacaggt agccattgcg
      601 tgacgacagc gccagtccgt cgtcggcgcg cacggtcggt ccgacgacga tctcgatcgg
      661 caatgcgaac tgctcgacca tctggcggat gatcatcagc tgttgataat ccttctggcc
      721 gaacacggca atctgtggct gcacgatgtt gaagagcttg agcacgaccg tcgccacgcc
      781 gcggaagtgg ccaggacgca ccgccccttc gagttcgttc tcgatcggtg gtaacgctac
      841 agaaaagacc tgcggcgatg gatagaggtc ggcctcgccc ggcgcgaaga cgaaatcgac
      901 gccggcggcg gccatctgct cgcagtcggc ggcaaaggtc ctcggatagc ggtcgaagtc
      961 ctcgttcggg ccgaactgta ggcgattcac gaagacgctg gagactacgg tgtcggccag
     1021 cgctcgtgcc cgttccatca gcgcgatgtg gccggcgtgc agattgccca tggtcggcac
     1081 gaaggcaatc gtgcctttac cgtaaagcgt gccgcgtacc gctgcgacgg tatcgagtac
     1141 gtccatcgtc aattggaatc agtaggtgtg ttcggccgcc gggaacgaac cgtcgcgaac
     1201 ggcggcgaca taagcggcta cggcgtcctg gaccgagctg gcaccggcca tgaagttgcg
     1261 cacgaagcgc ggcttcttgc cggccgtgag gtcgagcatg tcgtggagca cgagaacctg
     1321 gccactggtc gcgctgccgg cgccgatgcc gatcgtcggt acgtgcagcg cggtggtgat
     1381 ttcggcggcc agtttggcgg gaacggcctc catcagcacg aggctggcgc cggcggcttc
     1441 gagcgccgcg gcgtcggtgc gaaggcgggc cgcagaggct tcgtccttgc cctgcacccg
     1501 atagccgccg agctgatgca ccgattgcgg cgtcagtccg atgtgggcgc agaccggaat
     1561 gccgcgggtg gtcaggaagt tgacggtttc gaccatctca ttgccgccct cgatcttaac
     1621 catctgcgcg ccggcggcca tcagccgggc ggcggcgcga aacgattcct tggccgagac
     1681 ctggaaggtg ccgaagggca tgtcggcgat gatcagcggc cggcggcagc cgcgggtgac
     1741 gcaggcgacg tggtaggcca tatcgtcgag gctgaccggc aaggtcgagt cgtgcccctg
     1801 taggaccatg cctagcgagt cgccgacgag aatggtatcg acgtcggcgg cctcgagcag
     1861 cgccgcgaag gtcgcgtcgt aggcggtgag catggcgatg cgctgccctt cccggcgcat
     1921 gcggaacagg tcgtgcgtgg tcagtcgtcg ggcttgcgcc ggttgtacgg acatcgcgtc
     1981 tactctcccc ggttcaggta ttcgcgcggc ccgcgcatct gcgaaagccg ctcaacgagg
     2041 agtacgaaat cgttgtcatc atcgacgaag ttcaggtttt ccgaattgac gatcatcact
     2101 ggcgcctcgt cgtactggta gaagaagctt gcatagcgct cggcaagcag ggccaggtaa
     2161 ccctccccga ttcgccgctc actttcgatg ccgcgcttgc ggattcgcgc gatcagcgtt
     2221 tccggccgcg catgcaggta gaccaccagg tccggcgccg gcgcctgcgg cgcgagggta
     2281 ccgtaaatct gccgatagag ggcgtactcg tcgtcggcca gattgagctg ggcgaacagc
     2341 gggtccttgt cgagcaggta gtcggcaact acgcgcgagc cgaagatatc gggctgcgac
     2401 aggtcgcgca gttggtcgac gcgctggaac agaaagaaca gctgggtctg cagcgcgtag
     2461 cgcttggggt cttggtaaaa gcgcgcgagg aacgggttgg cgccgggctg ctcgagcagc
     2521 atgccggcct caagatgtgc cgccaggcgc atcgccagac tggtcttgcc ggcgccgatc
     2581 ggcccctcga cgacgatgtg gcgggcttcg gcaaggacgt tgcggacccg ggagtcggtc
     2641 atcggctcag gctgcgtgat ccaggcagag aatgtcgggc gagttttgcc cgtgcaccga
     2701 catgcgttcg aggcaggaca gcacctcggc gctggcggcc tccatgcgcg ccaattgcgc
     2761 agttgcggcg atcatctcgc ccttgtggtg caggttcacc gcctcgcggc cgaactgatg
     2821 cacctcgccg tgcggctttt ccatcgcacg gtagccgtcg aggtcggcaa agcattgctt
     2881 gccttcgccc tcgtagtacc acttgccgag acggcaggca gtatggctgg cgaactcctc
     2941 ggcgcgcttg gctgacacgc cggtgagcac cttgtagatc tcgaacttgt agatcaggtg
     3001 gtccatcttg gcgagttcgg taaagctctt tagcgccgc
//
LOCUS       DATDAA010000037         3169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_282285,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000037 DATDAA010000000
VERSION     DATDAA010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282285"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VIS73_01365"
     CDS             complement(<1..1166)
                     /locus_tag="VIS73_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEY5761836.1"
                     /translation="MEDIPIPALGGALVLLLFLSGCFSLTETAMMAANRYRLRHLAEQ
                     GSRGARLALALLARTDRMLGVILLGNNLVNTAAATLTSVIAINMFGEQEWALAVGTVL
                     LTFAILVFSEISPKIIGAYHADRLAVVLGYLLTPLLRIASPVVSFVNMFAMALLTVLR
                     LKPKESSSSMRLSPEELRTLVLESGQALPQQQQAVLLNLFELGRTRVEDVMTPRGAIE
                     AIDLSHSWPDLIRQLTTSHHTRLVVHDGDLDRVAGILHLRHVVGHINDPEWTVEQLRQ
                     ALQDAYYVPGQTPALAQLQLFRENRQRMGLVVDEYGELLGLVTPDDIIEEIVGELNAT
                     TTDLAQPLEWDEGGSVLVEGSRSLRELNRRLGLTLPVDGPITLNGLIIEYFQDIP"
     gene            complement(1171..2031)
                     /gene="ccsA"
                     /locus_tag="VIS73_01370"
     CDS             complement(1171..2031)
                     /gene="ccsA"
                     /locus_tag="VIS73_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY5761837.1"
                     /translation="MPILDQPPAAFALLALASAIYLVLAVHFFRTRWLASPTPASGAD
                     RAPLGLSGPEQGGIAAVLAIHAANLYFSMFGDDGMRFSFALAVSVMMWLAALTYWIEG
                     FRTRIEGMLPLVLGAAAVAVALPLLFGRTHTLEHAAAMAFRLHFLAAMLAYSLFTLAA
                     LHAVLMGIVERRLHRHAPGPTAMRLPPLLTLETLVFRMVWIAFVLLTIAVGSGVLSSE
                     SIYGRPFSLDHKTIFASLSWILVAILLAGRHFRGWRGKTALRWLLAGFIALILAYFGS
                     RFVAEIVLGR"
     gene            2065..>3169
                     /gene="ffh"
                     /locus_tag="VIS73_01375"
     CDS             2065..>3169
                     /gene="ffh"
                     /locus_tag="VIS73_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY5761838.1"
                     /translation="MLENLTQRLSKVMKTLRGEARLTEANIAEALREVRMALIEADVA
                     LPVVKDLIATIKEKALGEEVVGSLTPGQTLVGVVQRELAALMGGVHAGLNFATQPPAI
                     ILMAGLQGAGKTTTVGKLAKLLREQQKKKVLVVSCDVYRPAAIEQLKTVAAQAGADFF
                     PSAVGEKPVDIAAGAIDWAKKHFHDVLLVDTAGRLAIDDAMMAEIKALHAQIKPIETL
                     FVVDAMQGQDAVNTARAFGEALPLTGVVLTKLDGDSRGGAALSVRHVTGVPIKFAGVG
                     EKLSGFEAFHPDRMASRILGMGDVLSLIEEAKRGIDEQKAVDFAKKLKSGKGFDLTDF
                     KEQIGQMRKMGGLSSMMDKLPAQFAQAANQMQAG"
BASE COUNT          631 a          959 c         1062 g          517 t
ORIGIN      
        1 gggatgtcct gaaagtactc gatgatcagg ccattcagcg tgatcgggcc gtcaaccggt
       61 agcgtcagac caagccgccg gtttagttcg cgcagcgaac gtgagccttc gaccaggacg
      121 ctgccgccct catcccactc cagcggttgt gcgaggtcgg tggtcgtggc gttcagttcg
      181 ccgacgatct cctcgatgat gtcatcgggt gtcaccagcc cgagcagttc gccgtactcg
      241 tcgacgacga ggcccatgcg ctgccggttt tcgcgaaaca gctgcagctg cgccagcgcc
      301 ggagtctgcc ccgggacgta gtaggcgtcc tgcagggcct gacgcaactg ctcgactgtc
      361 cattccgggt cgttgatgtg gccgaccacg tgccgcaggt gcaggatgcc ggcgacgcgg
      421 tcgagatccc cgtcgtggac caccaggcgc gtgtggtggc tggtggtcag ttgccggatg
      481 agatcgggcc atgagtgcga caggtcgatc gcctcgatcg caccgcgcgg ggtcatgacg
      541 tcctcgacgc gggtgcggcc cagttcgaat agattgagca ggacggcctg ctgctgctgc
      601 ggcagggcct gcccagactc gagaaccagc gtgcgcagtt cctcggggga cagccgcatg
      661 ctgctgctgg actcctttgg cttgagccgg agcacggtca gcagggccat cgcgaacatg
      721 ttgacgaagg aaacgaccgg cgacgcgatg cgcagcagcg gcgtcagcag gtagccaaga
      781 accaccgcca ggcgatcggc gtggtacgcg ccgatgatct ttggtgaaat ctcggagaag
      841 accagaattg cgaaggtcag cagcacggtt ccgaccgcga gcgcccattc ctgctcaccg
      901 aacatgttaa tggcgattac gctggttagc gtcgcggcgg cagtgttgac gaggttattg
      961 ccaagcagga tcaccccgag catgcggtcg gttcgggcga gcagtgccag cgccagtcgc
     1021 gcgccgcggc tgccctgctc tgcaagatgc cggagccgat agcggttggc cgccatcatt
     1081 gccgtttcgg tcagtgaaaa gcagcccgac aagaacagca ggagcaccag cgcgccaccg
     1141 agggcgggaa tcggtatgtc ttccaaggcg ttatcggccg agcacgattt cggcgacgaa
     1201 acgactgccg aagtaggcaa gtatcagggc aatgaaacca gcgagcagcc agcgcaatgc
     1261 ggtcttccca cgccagcctc ggaagtggcg accggcgagc aggatggcga cgagaatcca
     1321 ggaaagcgac gcgaagatcg tcttgtggtc gagcgagaag ggccggccgt agatcgattc
     1381 cgaggacagc acgccgctgc cgacggcgat cgtcagcaac acgaaggcga tccagaccat
     1441 gcggaagacc agcgtttcca gcgtcagcag cggcggcaga cgcatcgccg tcgggcccgg
     1501 cgcgtggcgg tgcaggcgac gttcgacgat gcccatcagt actgcgtgca aggcagccag
     1561 cgtgaacagg ctgtaggcca gcatcgccgc gagaaaatgc aggcgaaatg ccatcgccgc
     1621 cgcgtgttcc agcgtgtgcg tccggccgaa cagcagcggt agcgccacgg caactgcggc
     1681 tgcgccgagt actagcggca gcataccttc gatgcgcgtg cggaatcctt cgatccagta
     1741 ggtcagcgcc gcgagccaca tcatgaccga tacggcgagg gcgaacgaaa aacgcatgcc
     1801 gtcgtcgcca aacatcgaaa aataaagatt cgccgcgtgg atcgccagta ccgctgcgat
     1861 tccgccctgt tctggtccgc ttagcccaag cggggcgcgg tcggcgccgc tggcgggcgt
     1921 cgggcttgcc agccatcgcg tacgaaaaaa atgcaccgcg agaacaagat aaatcgcgct
     1981 ggcgagggca agcagcgcaa aggcggcggg cggctggtct aaaatcggca tctcctagga
     2041 attctaacgt aagcaatcat ggccatgctc gagaatctga cgcagcgact ttccaaggta
     2101 atgaagacgc tgcgcggcga agcccgtctg accgaagcca atattgccga ggcgctgcgc
     2161 gaggttagga tggcgttgat cgaggccgac gtcgccttgc ccgtcgtcaa ggacctgatc
     2221 gccaccatca aggaaaaggc gctcggcgag gaagtcgtcg gcagcctgac gcctgggcag
     2281 acgttggtcg gcgtcgttca gcgtgagctg gcggcgctga tgggcggtgt gcacgccggc
     2341 ctcaactttg ccacgcaacc gccggcaatc atcctgatgg ccggcctgca gggcgccggc
     2401 aagaccacca cggttggcaa gctggcgaag ctcctgcgcg aacagcaaaa gaaaaaggtc
     2461 ctcgtcgtct cctgtgacgt ttatcgcccg gccgcgatcg agcagttgaa gacggtcgcc
     2521 gcccaggccg gtgccgactt cttcccctcg gcggtcggcg agaagccagt cgacattgcc
     2581 gccggggcca tcgactgggc gaaaaagcac ttccacgatg tcctgctggt cgataccgcc
     2641 ggtcgtctcg ccatcgatga cgcgatgatg gctgagatca aggcgctgca cgcccagatc
     2701 aagccgatcg aaacgctgtt cgtcgtcgac gcgatgcagg gtcaggatgc cgtcaatacc
     2761 gcgcgcgcct ttggcgaggc attgccgctg accggcgtcg tgctgaccaa gctcgacggc
     2821 gactcgcgcg gcggtgcggc gctgtcggtg cgccacgtga ccggcgtgcc gatcaagttc
     2881 gccggcgttg gcgaaaagct ctcgggtttc gaggccttcc accccgaccg gatggcctcg
     2941 cggatcctcg gcatgggcga cgtgctgtcg ctgatcgagg aggccaagcg cggcatcgac
     3001 gagcagaagg ccgtcgactt tgccaagaag ctgaagagcg gcaagggctt cgatctcacc
     3061 gatttcaagg agcagatcgg ccagatgcgc aagatgggtg gtttgtcgtc aatgatggat
     3121 aaactgccgg cacagtttgc gcaggctgcg aaccagatgc aggcagggg
//
LOCUS       DATDAA010000038         5205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_212381,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000038 DATDAA010000000
VERSION     DATDAA010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212381"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VIS73_01380"
     CDS             <1..346
                     /locus_tag="VIS73_01380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY5761839.1"
                     /translation="QQGVREDEKSVATKYALAEGAELFTRGLDRDAERDSDADGVLYA
                     ARAGYDPSACLNFMRRLAALKREAGTLEALYRTHPQAGERVADVRAALRRLQGAEPGT
                     GARPPLPQGDAR"
     gene            371..583
                     /locus_tag="VIS73_01385"
     CDS             371..583
                     /locus_tag="VIS73_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761840.1"
                     /translation="MPSIARLFQPRNPAFWLIIVLNALSALLAWVLRTYELAWWPALI
                     VVVFALGNAGLGLWLAWRLVRQPPPA"
     gene            613..1125
                     /locus_tag="VIS73_01390"
     CDS             613..1125
                     /locus_tag="VIS73_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HEY5761841.1"
                     /translation="MKKSPNTSIRLVTNEQLATLSQQAVASPRRRANHNLHPKLADPV
                     QRFLNAMEPGTYVRPHRHVTPGPKWELFVALSGAIAVLLFDDAGRVTERVVVAASGPV
                     RAIEVPPGIWHTVVVLEAGTVLFEFKQGPYASLSDKDFAHWAPAEGEPGASAMLERFL
                     AAAPGARLGD"
     gene            1187..1396
                     /locus_tag="VIS73_01395"
     CDS             1187..1396
                     /locus_tag="VIS73_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761842.1"
                     /translation="MIGVACFSPNGSTSGVNMPAISLRQIRGTKGIVVTYHLDKKDRL
                     EISGAFPHHWLRRSRHLNPNQENAT"
     gene            1393..1962
                     /gene="ahpC"
                     /locus_tag="VIS73_01400"
     CDS             1393..1962
                     /gene="ahpC"
                     /locus_tag="VIS73_01400"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HEY5761843.1"
                     /translation="MNTTMINTEVLPFKATAFHNGKFVEVSEASLKGKWSVVFFYPAD
                     FTFVCPTELGDLADHYDTFKSLGVEIYAVSTDTHFTHKAWHDSSETIGKIRYPMIGDP
                     TGTISRNFGVMIESAGLAERGTFVIDPNGRIQIIEINAGGIGRDARELLRKVEAAQYV
                     ANHPGEVCPAKWKQGEKTLAPSLDLVGKI"
     gene            2083..3669
                     /gene="ahpF"
                     /locus_tag="VIS73_01405"
     CDS             2083..3669
                     /gene="ahpF"
                     /locus_tag="VIS73_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEY5761844.1"
                     /translation="MLDANIRTQLAAYLEKLQAPIELIASLDDSPPADQMRELLGDIG
                     ELSAKVSVRFDGDDVRRPSFSVGRPGEPARIRFAGLPMGHEFTSLVLALLQTGGHPPK
                     VDPAMIEQVRAIKAPVGGKLRFETFISLSCHNCPEVVQALNLMAVLNPAIESVMIDGA
                     LFEEEVESRKIMAVPVAFLNGEPFGHGRMTIEEIVAKVDTGAATREAEKISGKDPFDV
                     LVVGGGPAGAAAAIYAARKGIRTGLVAERFGGQVLDTLGIENFISVQHTEGPKLVAQL
                     EQHVRQYEVDIINLQRAAELVPGDRFHEIKLAGGASLRAKSVVIATGARWRELEVPGE
                     AEYKGKGVCFCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVGHVTLLEYNDALRA
                     DAVLQAKLKSLSNVEVIVRAQTTEVVGDGEKVTALAYTDRAVGETRRIDLDGVFVQIG
                     LLPNTGWLKGTVALSRYGEIEIDARCETSVPGVFAAGDCTTVPYKQIVIAMGEGSKAS
                     LSAFDHLIRSPTLAETATAT"
     gene            3783..4046
                     /locus_tag="VIS73_01410"
     CDS             3783..4046
                     /locus_tag="VIS73_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761845.1"
                     /translation="MDECRQRDQREQVDPIPPDLYQRLTASQIFAIRRIESFGYELRF
                     VRRQGLPEAIPFVASPSVSTFGVVEPDGNINHKHTHVIRPSGG"
     gene            4118..5128
                     /locus_tag="VIS73_01415"
     CDS             4118..5128
                     /locus_tag="VIS73_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761846.1"
                     /translation="MTNPNRLSFAWPALALPWLLVADAALAGGSNYGVIPGALPAIKG
                     EAREWRVPTPEFARDPAPAPDGSIFIAVMHGNRIARFVPKTAQFTEWDLPKRARPHGL
                     VVDARGIVWYTGNGNGTIGRLDPQTGKVSEYRVPGGGDPHTIVIAEDGTFWFTVQNGQ
                     RVGHLDPVSGRIDEYPLSGNPYGLAIDRNGIVWVCRLRGDSLAWIDPKTGQRGEVDTG
                     SGSAPRRIATAPDGSLWVAYNGNGQLAKLDPAAKRIVKAWPLPSAGGYRAYAVTVDGA
                     GRVWVNGIDTDKVSLFDPASETFRVIELPSKGVGIRKAIIDADGRYWYMGSHNGRLGV
                     VE"
BASE COUNT          928 a         1674 c         1699 g          904 t
ORIGIN      
        1 gcagcagggc gtccgcgaag acgaaaagag cgtcgcgacg aaatacgcgc tggccgaggg
       61 cgccgagttg ttcacccgcg ggctcgaccg cgacgctgag cgcgattccg acgccgacgg
      121 ggtgctctac gcggcgcggg ccggctacga tccgtcggcc tgcctcaact tcatgcggcg
      181 tcttgcggcg ctgaagcgcg aggcgggtac gctcgaggcc ctgtaccgaa cccatccgca
      241 ggccggcgaa cgcgtcgccg atgtcagggc ggcgctgagg cgactgcagg gcgcggagcc
      301 gggaacgggc gctcggccgc cgctgccgca gggcgatgcc cggtagggcg catacgctgc
      361 aagcaacagg atgccgtcga tcgcccgcct ctttcagccg cgcaatcccg ccttctggct
      421 gatcatcgtg ctcaacgcgc tgtcggcgct gctcgcctgg gtgttgcgta cctatgaact
      481 tgcctggtgg ccggcgttga tcgtcgtcgt cttcgcgctc ggcaacgccg ggctcggcct
      541 ctggctggcc tggcgcctgg ttcgccagcc gccgccggcg tgatctgccg tgatgctcgg
      601 cgtcccagtg ccatgaagaa gtcgcccaac acgtcgatcc ggcttgttac caacgagcaa
      661 cttgcgacgc tgtcgcagca agcagtcgcc tctccgcgtc ggcgcgccaa ccacaatctg
      721 catccgaagc tcgccgatcc ggtccagcga ttccttaacg ccatggagcc gggcacctac
      781 gtgcggcccc atcggcacgt gacgccgggc ccaaaatggg agctattcgt cgccttgtcc
      841 ggggcgatcg cagtgctgct tttcgacgat gccggtcgcg ttaccgagcg ggtggtcgtt
      901 gcagcatcag gcccggttcg agcgatcgag gtgccaccgg ggatctggca tacggtggtg
      961 gtgcttgaag ccggcaccgt actcttcgag ttcaagcagg gaccctacgc gtcgctgagc
     1021 gacaaggact tcgcgcactg ggcgccggcc gaaggtgagc ccggcgcgtc ggcaatgctg
     1081 gagcgcttcc tcgctgcagc gccgggcgca cgcctcggcg actgatcacg ctgcggccat
     1141 caggtcgcct tcgctgtgtt acgcgctttt cacgatcaag cgaccgatga tcggcgtcgc
     1201 ttgcttctcg ccaaatggct ccacgagcgg cgtcaacatg ccggcaattt cgttgcggca
     1261 aattcgcggc accaaaggca tagttgttac ctatcacttg gataaaaaag atcgtctgga
     1321 gatatcaggt gcgttccccc atcattggtt gcgtcgcagc agacatctca accccaacca
     1381 ggagaacgca acatgaacac cacgatgatt aataccgaag tcctcccctt caaggcaact
     1441 gccttccata acggcaagtt tgtcgaggtc agcgaggcaa gcctgaaggg caaatggtcg
     1501 gtggtcttct tctaccccgc tgacttcacc ttcgtctgtc cgaccgaatt gggagacttg
     1561 gctgatcact acgacacctt caagtctttg ggcgtcgaga tctacgcggt gtcgaccgac
     1621 acgcacttca cgcacaaggc ctggcacgac agctccgaaa cgatcggcaa gatccgctac
     1681 ccgatgatcg gcgaccccac cggtacgatc agccgcaact tcggcgtgat gatcgagagt
     1741 gccggcctgg cggagcgtgg caccttcgtc atcgacccga acggcaggat ccagatcatc
     1801 gagatcaacg ccggcggcat cggtcgcgac gcccgggagc tgctgcgcaa agtcgaggcc
     1861 gcgcaatacg tcgccaacca cccgggcgag gtctgcccgg cgaaatggaa gcagggcgag
     1921 aagacactgg cgccctcgct cgacctggtt ggcaagatct gatctagctc gccaactcag
     1981 tcaatcctgc cccggatcct ggggcgggat tgcagcgcag agcccggctg gcgacagcgc
     2041 gctctgccct gcaatccgaa acaagccaca cgggagaaca ccatgctcga cgccaacatc
     2101 cgaacccagc ttgccgctta ccttgaaaaa cttcaggcgc cgatcgaact gatcgcttcg
     2161 ctcgacgaca gtccaccggc agaccagatg cgcgaactgc ttggcgatat cggcgagctg
     2221 tcggcgaaag tgagcgtacg cttcgatggc gacgacgtac gtcgcccgtc gttctcggtc
     2281 ggtcggcccg gcgagccggc gcgcatccgc ttcgctggcc tgccgatggg ccacgaattc
     2341 acctcgctgg tgctggcgct gcttcaaacc ggcggccatc cgccgaaggt cgacccggcg
     2401 atgatcgagc aagttcgggc gatcaaggcg ccggtcggcg gcaagctgcg cttcgagacc
     2461 ttcatctcgc tgtcctgcca caactgcccg gaggtcgtgc aggcgcttaa cctgatggcg
     2521 gtactcaacc ccgccatcga gagcgtgatg atcgacggcg ccttgttcga ggaggaagtc
     2581 gagtcgcgca agatcatggc ggtgccggtt gctttcctca acggcgagcc cttcggccac
     2641 ggtcggatga cgatcgaaga gatcgtcgcc aaggtcgaca ccggcgcggc gacgcgcgag
     2701 gctgagaaga tctccggcaa ggatccgttc gacgtcctgg tcgtcggcgg cgggccggcc
     2761 ggcgcggcgg cggcgatcta cgccgcgcgc aagggaatcc gtacgggctt ggtcgccgag
     2821 cgtttcggtg gccaggtgct cgacacgctg ggtatcgaga acttcatctc ggtgcagcac
     2881 accgaggggc cgaagctcgt cgcccagctc gaacagcacg tgcgccagta cgaggtcgac
     2941 atcatcaatc tgcagcgtgc cgccgaactg gttcccggcg atcgtttcca cgaaatcaag
     3001 ctcgctggtg gtgcgtcgtt acgtgccaag agcgtcgtca tcgccaccgg cgcgcgctgg
     3061 cgcgagctcg aggtgcccgg cgaggccgag tacaagggca agggcgtctg cttctgcccg
     3121 cattgcgacg ggccgctgtt caagggcaag cgcgtagcag ttatcggcgg cggaaactcg
     3181 ggtgtcgagg cggcgatcga cctcgccggc atcgttggcc acgtgacgct gctagagtac
     3241 aacgacgcgt tgcgtgctga cgcggtgctg caggcgaagc tgaagagcct ttctaatgtc
     3301 gaagtcatcg tccgcgcgca gaccaccgaa gtggtcggcg acggtgaaaa ggtaacagcg
     3361 cttgcctaca ccgatcgtgc cgtgggtgag acccggcgca tcgacctcga cggcgtcttc
     3421 gtgcagatag gcctgctgcc gaacaccggc tggttgaagg gcacggtcgc actgtcgcgt
     3481 tacggggaga tcgagatcga tgcccgctgc gagacctcgg tgcccggcgt tttcgctgcc
     3541 ggcgattgca cgacggtgcc gtacaagcag attgtcatcg cgatgggcga ggggtcgaag
     3601 gctagcctat cggcctttga ccatttgatc aggagtccta ccttggccga aacggctaca
     3661 gcgacctgaa cgggggggca gcctagccgg aggctattcc cgagggggta tgacgtatgt
     3721 ttggctggac aaaacctgcc gattgccgat aatgcgggaa ataccaccgt ttggagccga
     3781 ctatggatga atgtaggcag cgagaccagc gcgagcaggt cgacccgata ccgcccgatc
     3841 tctatcagcg gcttaccgca agccagatat tcgcgatccg acgcatcgag agcttcggct
     3901 atgaactgcg tttcgtccgc cggcaggggc tgcccgaggc gatccccttc gtcgcgagcc
     3961 cgagcgtgtc gaccttcggg gtcgtcgagc ccgacggtaa catcaatcac aagcacactc
     4021 acgttatccg ccccagcggc ggctaactgc cggcggcgct gaaacgccgc tgcatccgcg
     4081 ctgtcccacg gcttcgattg gcagcagcgg gggcagagtg acaaacccga atcgtctttc
     4141 gttcgcctgg ccggcgttag cgctgccttg gctactcgtc gccgacgcgg cgctggccgg
     4201 cggttccaac tacggcgtaa ttcccggggc cttgccggcg atcaagggcg aggctcgcga
     4261 gtggcgcgtg ccgacgcccg agttcgcgcg cgacccggcg ccggcgcccg acggcagcat
     4321 cttcattgcg gtgatgcacg gcaatcgcat cgcccgcttc gtgccgaaga ccgcgcagtt
     4381 caccgagtgg gacctgccga agcgtgcccg gccgcacggt ctggtcgtcg atgcacgggg
     4441 aattgtctgg tacacgggca acggcaatgg cacgatcggc cgcctcgatc cgcagaccgg
     4501 caaggtcagc gagtaccgcg tacctggtgg tggcgatccg catacgatcg tgatcgccga
     4561 ggatgggacc ttctggttta ccgttcagaa cggccagcgt gtcggtcacc ttgacccagt
     4621 cagcggccgc atcgacgaat atccgctgtc gggcaatccc tacgggctgg caatcgaccg
     4681 caacggcatc gtctgggtct gccgtctgcg tggcgactcg ctggcctgga tcgatccgaa
     4741 gaccgggcag cgcggcgaag tcgataccgg ttctggatcg gcgccgcggc ggatcgcgac
     4801 ggcgcccgac ggcagcctgt gggttgccta caacggcaac ggccagctgg cgaaactcga
     4861 tccggcggcg aaacgcatcg taaaggcttg gccgctgccc agcgccggcg gctatcgcgc
     4921 ctacgcggtc accgtcgatg gtgccgggcg cgtctgggtc aacggcatcg acaccgataa
     4981 agtctcgctc tttgatccgg cgagcgagac attccgcgtc atcgagctgc cctcgaaagg
     5041 cgtcggcatc cgaaaggcaa tcatcgatgc cgatggccgc tactggtaca tgggcagcca
     5101 caacggccgg ctgggggttg tcgagtgatc gcgctgcgta cctgctgggc agcgatcgcg
     5161 ctctgggccg gcctgttgcc gttcgcggca caggcgcagg caacg
//
LOCUS       DATDAA010000039         7383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_456933,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000039 DATDAA010000000
VERSION     DATDAA010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456933"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VIS73_01420"
     CDS             <1..1081
                     /locus_tag="VIS73_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5761847.1"
                     /translation="GAASQSKVLAGVQTLWRPNTPQLYVDLDREKAKALGVRVDDAFN
                     SLAGTLGTLYVNDFNKYGRAWQVLMSADAGFRMKPEDVGRIYVKSDRGAMVPISAIAK
                     IDYSAGPDSLDRYNNLPAVKMLGAAAPGFSSGQAIAEVERLAAQALPPGLSYEWTGAS
                     FQEKRDSGASGIALGAAVLMVFLILAALYEKWSLPLSVLLALPFGTFGALVAVWVAGL
                     TNDVYFQIGLVTLLGLAAKNAILIVEFALMKFNEGWSAGAAALEAARLRFRPIIMTSL
                     AFILGVTPLAFSSGAGAGARHSVGTGVMGGMMAATFLAVTFLPVFFYWLTERRITETR
                     SRKELADEIAARHRAEHVATEEGLA"
     gene            1078..2445
                     /locus_tag="VIS73_01425"
     CDS             1078..2445
                     /locus_tag="VIS73_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY5761848.1"
                     /translation="MRHAAMTRLVSIVAALLLAGCASLAPDYATPAVELPPDMPIAEP
                     KVPWWETGSDAALRGLIEDALAHNADLLIAAARTEEARSALGIARADQWPDAGVQLGA
                     SRERDQTLLPGTRERYSAQLLVSWEVDLWGRYRDASTAAREQLFAAEAGREAFRLSLA
                     SQVAQTWYALQALTQQSALAGQVLATQEKALGLLRQRVDAGIAGEFELRQQEAEVAGT
                     RVIQQQLLAARTQATNALGILLGRSPQALVEGQLPQGGAIAVLGVPPSLPEGLPSTRL
                     LNRPDVQAAEANLKAANAQIGVARSAWFPSLSLTVAGGGASRELSTLFDSASRTFSVA
                     GALLQPLAYSGRISAGIDAARAGRDAAELGYRQTLARAFGEVLDAIAARQSAREIQLA
                     ESTRVAALTETLRIARRRFEAGLTSQFELLGSERALLAAQGNLIDAQRAEAAAAVQLW
                     TALGG"
     gene            2460..3014
                     /locus_tag="VIS73_01430"
     CDS             2460..3014
                     /locus_tag="VIS73_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEY5761849.1"
                     /translation="MDGNLDTPEAVLHFWFKEIEAAKWWRKDAEFDRLIGQRFGALHA
                     RGADGELFGWRSTPGGRLAEVIVLDQFSRNLYRDSMLAFAFDGMALVLAQEAVAAGAD
                     VALPTEQRAFLYMPYMHSESAAIHRVAVELFSAPGLEGSLDFELRHKAIIDRFGRYPH
                     RNAVLGRESTAVELAFLKEPGSSF"
     gene            3188..4579
                     /gene="nifE"
                     /locus_tag="VIS73_01435"
     CDS             3188..4579
                     /gene="nifE"
                     /locus_tag="VIS73_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifE"
                     /protein_id="HEY5761850.1"
                     /translation="MSALKQRIDGLLQEPGCSTNQAKSDKARKAGCKKPLTPGAAAGG
                     CAFDGAKIALQPVVDVAHLVHGPIACEGNSWDSRHVYSSGAQTYRTGFTTDMAEFDVI
                     YGGEKRLYRAIREIIEKVDPPAVFVYQTCLPAMIGDDVEGVCKAAAEKFGKPVIPINA
                     PGFAGSKNLGNKLGGEALLDHVVGTMEPDYTTDCDINIIGEYNVVGEFWQVKPLFDEL
                     GIRVLSTFCGDARYRDIASSHRARAAIMLCSQALINVARKMDERYDIPFFEGSFYGVT
                     DMSDCLRRIAALLVGKGARRDLLDRTEALIAREESRAWARLERYRARLQGKRVLLFTG
                     GVKSWSVVSALREAGMQIVGTSMRKSTQHDRDKVIEIMGTDAHMFDELPPREMYRMLK
                     ESKADVMLSGGRSQFVALKAKTPWVEINQERHHPFAGYQGIVNLVHEIDKALYNPIWE
                     QVRTPAPWEVDGE"
     gene            4614..4769
                     /locus_tag="VIS73_01440"
     CDS             4614..4769
                     /locus_tag="VIS73_01440"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEY5761851.1"
                     /translation="MEGSLNFELRHTAIIDRFGRYPHRNAALGCESTALELPFLRSLV
                     QASRRAA"
     gene            4857..5597
                     /locus_tag="VIS73_01445"
     CDS             4857..5597
                     /locus_tag="VIS73_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY5761852.1"
                     /translation="MRTALLADIHSNLEALRACLAHAEAQGATDFAFLGDIVGYNADP
                     RACLDIVREYAARGAVVVLGNHDAAVLGGLLENMGFVAREAIYWTRAQLGESECAFLS
                     GLPLIVRREDQTFVHSSALAPQSWTYISSLRHARDCLLAANTPLTFAGHVHQQSLHYL
                     NGDTAFVHRPSVAMAIPLPSRWRWLAIAGSVGQARDGHAAAAYAVYDDSRREMRFFRV
                     SYDTRRAAAKVRAAGLPERLALRLLNGN"
     gene            5607..7019
                     /locus_tag="VIS73_01450"
     CDS             5607..7019
                     /locus_tag="VIS73_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY5761853.1"
                     /translation="MTLAQGDLIDGHRVVEVLPQGGMATLYRVQAPDGEQRLLKLPKL
                     AFGSHPACFAGFETERLLLERLTGPHVPRLFASGEVDGQAYLIIEDLGGDTLAKRMAQ
                     GALQIDEAVRLALLLASALAALHRQDVVHHDVKPSHVFFRRDGCAVLIDFGLACHAHL
                     PDFVGDAPQDRLAVLGTPGYVAPEQIAGRRGDPRSDIFSLGVVLYEMVTGALPFVAGG
                     SRLGMSLSRLFDCVPPGIRNPACPDWLQEVILRCIEPQRVQRYGSAALVATDLHHPEQ
                     IVVGERGKRRGRRFGVALQRWWHIVHEADPVERVVRAEVERAPQILVAIDTAHDDPAL
                     TGSMRATLCRLVAGAPQWRVICATVVSPVSEAGMEDVDDLERSRRSEILAALHHWAVP
                     LDCASEQLRFIVLDGSDAATTLVTYARHHHIDQILIGARGSSTVRRLLGSVSARVAAE
                     APCSVTVVRRGQDSHPDADD"
BASE COUNT         1209 a         2336 c         2494 g         1344 t
ORIGIN      
        1 gggcgcggcg agccagagca aggtgctcgc cggcgtgcag acgctgtggc gaccgaacac
       61 accgcagctc tacgtcgacc tcgaccgtga aaaggccaag gcgctcggcg tgcgcgtcga
      121 cgacgctttc aattcgttgg ccggcacgct cggcacgctc tacgtcaacg acttcaataa
      181 gtacggccgc gcctggcagg tgctgatgtc ggccgacgcc ggtttccgaa tgaagcccga
      241 ggacgtcggg cgcatctacg tgaagagcga tcgtggtgcg atggtgccga tctcggcgat
      301 cgcgaagatc gactacagcg ccggacccga cagcctggat cgctacaaca acctgccggc
      361 ggtcaagatg ctcggcgccg cggcgcctgg attcagctcg ggacaggcga ttgccgaggt
      421 agagcggctg gccgcgcagg cgctgccgcc aggcctgagc tacgaatgga ccggggcgtc
      481 gttccaggag aagcgtgact cgggcgcgag cgggatcgcc ctcggtgcgg cggtgctgat
      541 ggtctttttg atcctcgccg cgctctacga aaagtggtcc ttgcccttgt cggtgctgct
      601 cgcgctaccg ttcggcactt tcggcgccct ggtggcggtg tgggtcgccg ggctgaccaa
      661 cgacgtctat tttcagatcg gtctcgtcac gctgctcggg ctcgcggcga agaacgcgat
      721 cctgatcgtc gagttcgcgc tgatgaagtt taacgaaggc tggtcggcgg gtgcggcggc
      781 actggaagcg gcgcgcctgc ggttccgccc gatcatcatg acttcgctcg ccttcatcct
      841 cggcgtcacg ccgctggcct tctccagcgg tgccggcgcc ggtgcgcggc acagcgtcgg
      901 gaccggcgtc atgggcggga tgatggcggc gaccttcctc gccgtaacct tcctgccggt
      961 gttcttctat tggctcaccg agcgacggat caccgagacg cgctcgcgca aggaactcgc
     1021 cgacgagatc gccgcgcggc accgcgccga gcatgtcgcc accgaggagg gcctggcatg
     1081 agacacgctg cgatgacgag gctcgtgagc atcgtggccg cgcttctgct cgccggttgc
     1141 gcaagcctgg cacccgacta cgcgacgccg gcagtcgaac tgccgcccga catgccgatc
     1201 gccgagccga aggtgccgtg gtgggaaacc ggcagcgacg cggcgctgcg tgggctgatc
     1261 gaggatgcct tggcgcataa cgcagacctg ctgatcgccg ccgcccgcac ggaggaggca
     1321 cggtccgcgc tcggcatcgc ccgcgccgac cagtggcccg atgccggcgt tcagctcgga
     1381 gccagccgcg aacgcgacca gaccttgctg ccgggaaccc gggagcgtta cagcgcgcag
     1441 ttgctggtca gctgggaagt cgatctctgg ggacgctatc gcgatgcgag caccgcggcg
     1501 cgcgagcagc tgtttgccgc cgaagcgggc cgcgaggcat ttcgcttgtc gctggccagc
     1561 caggtcgcgc agacctggta cgcgctgcag gcactgacgc aacagtcggc acttgccggg
     1621 caggtcctgg cgacgcagga gaaggcgctg gggctgctgc gccagcgcgt cgacgcgggt
     1681 attgccggtg agttcgagct gcgccagcag gaagccgagg tggccggcac gcgtgtaatt
     1741 cagcaacagc tgctcgcggc gcggacgcag gcaaccaatg cgctgggcat cctgctcggc
     1801 cgttcaccgc aggcgctggt cgagggccag ctgccgcagg gcggggcgat cgcggtgctc
     1861 ggagttccgc cgtcgttgcc cgaagggctg ccctcgacgc ggctgcttaa tcgcccagac
     1921 gtacaggccg ccgaggcaaa cctcaaggct gccaacgccc agatcggcgt tgcccggtcg
     1981 gcctggtttc cgtcgctgtc gctaacggtg gctggcggcg gcgccagccg cgagttgtcg
     2041 acgctgttcg attcggcgtc gcgcaccttt tcggtcgccg gcgcgttgct gcagccgctg
     2101 gcctattcgg gtcgtataag cgccggcatc gacgccgcga gggcgggtcg cgacgctgcc
     2161 gagcttggct accgacagac cctcgcccgc gccttcggcg aagtgctcga cgcgattgcg
     2221 gcgaggcagt cggcgcgcga gatccagctt gccgaatcga cgcgagttgc ggcattgacc
     2281 gaaacgctgc gcatcgcgcg ccgccgcttt gaggccgggc tgaccagcca gttcgaactg
     2341 ctcggcagcg agcgggcgct gctcgcggcg cagggcaacc tgatcgatgc gcagcgcgcc
     2401 gaagccgcgg ctgcagttca actgtggacc gcgctcggcg gctgacggag ggaaaacaaa
     2461 tggacggaaa cctggatacg cccgaagcgg tgctgcactt ctggttcaag gaaatcgaag
     2521 cggcgaaatg gtggcgcaag gatgcagagt tcgatcgcct gattggccag cgtttcggcg
     2581 ccttgcacgc gcgcggcgcc gatggcgagt tgttcggctg gcggtcaacg cccggcggaa
     2641 ggcttgccga ggtgatcgtt ctcgaccagt tctcgcgcaa cctgtatcgc gattcgatgc
     2701 ttgcctttgc cttcgatggc atggcgctgg tgctggcgca ggaggctgtg gcggcgggtg
     2761 ccgatgtggc cctgccaacc gaacagcgtg cctttctcta catgccgtac atgcacagcg
     2821 aatcggcggc aatccatcgg gtcgccgtcg agcttttttc ggcgccgggg ctggaaggca
     2881 gtctcgattt tgagctgcgg cacaaggcga ttatcgaccg ctttggtcgt tatccgcacc
     2941 gcaacgcggt tctcgggcgc gagtcgacgg ccgtggaact ggctttcctc aaggagcccg
     3001 gcagcagttt ctaatctgct gcctcatcgg cgattaccct tgtcgtgccc gcgacggcgg
     3061 gcattgtcgg aaacccgacg cgaggccggc tcttggatgc tatccatctg agccgactgg
     3121 ggtttcgagc gctggcgccg ttcttgcatc ctgggtgtcg aggcgatgga cgccgggaaa
     3181 gttcgacatg agcgcactca agcaacggat cgacggactc ctgcaggagc ccggctgtag
     3241 taccaaccag gcgaaaagcg acaaggcgcg caaggcaggc tgcaagaagc cgctgacgcc
     3301 gggcgcggcg gccggtggct gcgccttcga cggcgccaag atcgcgttgc agccggtggt
     3361 cgatgtcgcg catctggttc acggcccaat tgcctgcgag ggcaattcct gggattcgcg
     3421 ccatgtctat tcgtcgggcg cgcagaccta ccgcacggga tttactaccg acatggccga
     3481 gttcgacgtc atctatggcg gcgagaagcg gctttaccgt gcgatccgcg agatcatcga
     3541 gaaggtcgac ccgccggcgg tcttcgtcta ccagacctgc ctgccggcga tgatcggcga
     3601 cgatgttgaa ggcgtctgca aggcggccgc cgagaagttc ggaaaaccag tgatcccgat
     3661 caacgcgccg ggattcgcgg gcagcaagaa cctcggcaac aagctcggcg gcgaggcgct
     3721 gctcgaccac gtggtcggga cgatggagcc tgactacacc accgactgcg acatcaacat
     3781 catcggcgag tacaacgtcg ttggcgagtt ctggcaggtc aaacccctct tcgacgaact
     3841 cggcattcgc gtgttgtcga ctttctgcgg cgacgcgcgc taccgcgaca tcgccagctc
     3901 gcaccgagcg cgggcggcga tcatgctctg ctcgcaggcg ctgatcaacg tcgcgcgcaa
     3961 gatggacgag cgctacgaca ttcctttctt cgagggcagc ttctacggcg tgaccgacat
     4021 gtccgattgc ctgcgccgga tagccgcgct gctcgttggc aagggtgccc gccgcgacct
     4081 gctcgaccgc accgaggcgt tgattgcccg cgaggagtca cgcgcctggg cacgcctcga
     4141 gcgctaccgg gctcgtctgc agggcaagcg cgttctgctg tttaccggcg gcgtgaaaag
     4201 ctggagtgtg gtctcggcac tgcgcgaggc cggcatgcag atcgtcggta cttcgatgcg
     4261 caagtcgact cagcacgacc gcgacaaggt catcgagatc atggggaccg acgcgcacat
     4321 gttcgatgaa ctgccgccgc gggaaatgta ccggatgctc aaggagtcga aggccgacgt
     4381 gatgctctcc ggcggccgct cgcagttcgt cgcgttgaag gcgaagacgc cctgggttga
     4441 gatcaatcag gagcgccatc atccgttcgc cggctaccaa ggcatcgtca acctcgtgca
     4501 cgagatcgac aaggcgctct acaacccgat ctgggagcag gtcaggacgc cggcgccatg
     4561 ggaagtcgac ggcgaataga cgagtcgccg tcgagctctt ttcggcgccg gggctggaag
     4621 gcagtctcaa ttttgagttg cggcacacgg cgattatcga tcgctttggt cgttatccgc
     4681 accgcaacgc ggctctcggg tgcgagtcga cggccctgga actgcctttc ctaaggagcc
     4741 tggttcaggc ttctaggcgc gccgcctgag cgccggcgcg ctcggggcgt cgccgggccc
     4801 cggtgctgcc cggcggcacg cgattctggt accgttggcg cgggaggtgc ggcaggatga
     4861 gaaccgcgct gctggcagac attcacagca atctcgaggc gctgcgcgcg tgcctagccc
     4921 atgccgaagc gcagggcgcg accgatttcg ctttcctcgg tgacatcgtt ggctacaacg
     4981 ctgacccgcg cgcctgcctc gacatcgttc gcgaatacgc ggcccgtggc gcagtggtcg
     5041 tcctcggcaa ccacgacgcg gcggtccttg gcggcctcct cgagaacatg ggttttgttg
     5101 cccgcgaagc gatctactgg acccgtgcgc agcttggcga gagcgaatgc gccttcctct
     5161 ccggactgcc gctgatcgtc cgtcgcgaag accaaacctt tgttcattcc agtgccctgg
     5221 cgccgcaatc ctggacctat atcagcagcc tgcgccatgc tcgcgactgc ctgctcgcag
     5281 ccaatacgcc gctgaccttc gccggccatg tccatcaaca gtctctgcac tatctgaacg
     5341 gcgacactgc cttcgttcac cgaccttcgg tggcgatggc cattccgctg ccgtcacgct
     5401 ggcgctggct cgccattgcc ggatccgtcg ggcaggcccg cgatggccat gctgcggcgg
     5461 cctatgccgt ctacgatgat tcacggcgcg agatgcggtt tttccgtgtt tcctacgaca
     5521 cgcgccgtgc ggcggcgaaa gtccgcgccg ccggattgcc cgagcggctt gccttgaggc
     5581 tgctcaatgg caactaaggg acggaaatga cactggctca aggtgatctc atcgacgggc
     5641 atcgtgtcgt cgaggtgctg ccccagggcg gcatggcaac gctgtatcgc gttcaggcgc
     5701 ccgatggcga gcaacgcctg ctcaagctgc ccaagttggc cttcggcagc catccggcct
     5761 gctttgcggg attcgagacc gagcgcctgt tgctggagcg gctgaccggc ccccacgtgc
     5821 cacgcttgtt cgcgagcggt gaggtcgatg ggcaggccta cctgatcatc gaggacttgg
     5881 gcggcgacac gctggcgaaa cgcatggcgc agggcgcctt gcagatcgac gaggcggttc
     5941 gtcttgcgct gttgctcgcc tcggcactcg ccgcactgca ccggcaggac gtcgttcacc
     6001 acgatgtaaa gccctcgcac gtattttttc gccgcgacgg gtgcgcggtg ctgatcgact
     6061 ttggcttggc ctgtcacgcg cacctgccgg atttcgttgg cgatgcacca caggatcggc
     6121 ttgccgttct cggcacgccg ggctacgtcg cacccgagca gattgccggc cggcgtggcg
     6181 acccgcgcag cgatatcttc tctttgggcg tcgtgctcta cgagatggtg accggcgccc
     6241 tgccgtttgt ggcaggcggc agccgtctgg ggatgtcctt gagccgcctg ttcgactgcg
     6301 taccgcctgg catacgcaat cccgcctgcc cggattggct gcaggaggtg attctccgtt
     6361 gcatcgaacc gcagcgggtg cagcgttacg gcagcgccgc gctggttgcg accgatctgc
     6421 atcatcctga gcagattgtc gtcggcgagc gcggcaagcg tcgtggtcgg cgattcggcg
     6481 ttgcgctgca gcgatggtgg cacattgttc acgaggccga tccggtcgag cgcgtggtgc
     6541 gcgcggaggt cgagcgggcg ccgcagatct tggtggcgat cgatacggcg cacgacgatc
     6601 cggcattgac cggcagcatg cgcgcgacgc tatgccgact ggtcgccggg gcaccccaat
     6661 ggcgggtcat ctgcgcgacg gtcgtcagcc cggtatcgga ggcgggaatg gaggacgtcg
     6721 atgatctcga gcgcagtcgc cgcagcgaaa tcctcgccgc gctgcatcac tgggccgttc
     6781 cgttggactg cgcatccgag cagctccgct tcatcgtcct cgatggcagc gatgcagcga
     6841 cgacgctcgt gacctacgcg cgtcaccacc atatcgatca gatcctcatc ggagcccgcg
     6901 gcagttcgac cgttcgccgt ctcctcggca gcgtgtcagc gcgagttgcg gcagaagcac
     6961 cgtgctcggt gacggtggtt cgccgtgggc aggacagtca tcctgacgcc gacgactaag
     7021 gcgcgcacga gcatggcgaa cccgtttggc gctgctctta ttgcgatgca gcttgacagc
     7081 ctaaagaggt tacggactat tctgcgacaa cacgatagaa gtcggccaaa ctggaccgga
     7141 ctcgacggag agagagaagt ctaagtagag gaggtgctat gacgcgtccg gttgctgtat
     7201 ttcggggcga ctcatagatg cttgagctcc aaatgaacgg agccgcacgc catgcgcgta
     7261 cggctccgtt ttatttttta attaggagga tgctgtcgtg atctatacaa gaaaatctcc
     7321 agccttgttg attatcggct tggccatact gctgtggggc tggctcaatg catcgggcag
     7381 cgt
//
LOCUS       DATDAA010000040         3639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_72557,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000040 DATDAA010000000
VERSION     DATDAA010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72557"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VIS73_01455"
     CDS             complement(<1..413)
                     /locus_tag="VIS73_01455"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302143.1"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7 phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate, tyrosine
                     sensitive; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY5761854.1"
                     /translation="MTQQNLDNINVAAFDEMPSPQAIHARLPITVDAEATVSNGRDTI
                     RRILDGEDDRLFVVVGPCSIHDPKAALDYARRLKALADDVADTLFIAQLRPEPGRVLL
                     CEQVETCTRNRGHTNEDEQALEQGVEFAREPRIFTT"
     gene            complement(456..>3639)
                     /gene="mfd"
                     /locus_tag="VIS73_01460"
     CDS             complement(456..>3639)
                     /gene="mfd"
                     /locus_tag="VIS73_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY5761855.1"
                     /translation="LATLYALTQGDCDLIIAPITTAVVRLPPASYLAAHAFMIRKGDR
                     LDLTKLRQQCATAGYHAVSQVMSPGEFCVRGGLIDLFPSGSVIPYRIDLFDDEVEQIR
                     CFDVDTQRSLYPVGEIRLLPAREFPLDEDGRSSFRQRFREHFVNDPTKTTIYRDVSSG
                     IATAGIEYYLPLFFEQTATLFDYLPADAAVCLHRDVQGALATFWKETRSRFELLRHDR
                     DRPLLEPALLFLDEESLFGQIRDRARVSIAPPPGAAGDPAGEMPTRPLPELAIDRRAD
                     DPTHRLRAFRADFAGAILIAAESPGRRESIAELLRDQRIAAIETVGAPDATDGDAPGR
                     ITLQAGATQLTVAPLQSGFIWGELAVVAEADLFPGTASRGTRDSRRRMSSENWLRDLT
                     ELKVGDPVVHEAHGIGRYQGLLHLDLGEGDMEFIELHYAGEAKLYVPVAQLAVLSRYT
                     GADPDTAPLHALGSPQWEKAKRKAALQARDVAAELLALYAARNARQGHAFEFSLNDYE
                     AFAEGFGFEETPDQQAAIAAVLADMRSGKPMDRLVCGDVGFGKTEVALRAAFAAVAGG
                     HQVAVLCPTTLLCEQHFRTFSDRFAGWPVNIAELSRFRSAKESTEALAGLAAGKIDIV
                     IGTHKLIQSDVRFAKLGLVVIDEEHRFGVRQKESLRRLRAEVDVLTLTATPIPRTLAM
                     SLEGIRDFSVIATAPQKRLSIKTFVTRLAEGTIREAILRELKRGGQVYFLHNEVATIE
                     NMRERLARIVPEARIAVGHGQMGGVELERVMREFTHGRANVLLCTTIVETGIDIPNAN
                     TILIDRADKFGLAQLHQLRGRVGRSHHQAYAYLLVDDEQGVNKQARQRLEAIQMMEEL
                     GSGFYLAMHDLEIRGAGEVLGDSQSGEMEAVGFALYSEMLNRAVAALKAGNEPDLTLP
                     SAIGTEINLHEPALLPDIYCADVHERLNLYKRLAHCTDDESLRDVQEELIDRFGELPP
                     QARCLIESHRIRIRSGTFGIVKLDAGIDRLLVQFSPKPPLDPMKIIKLVQTRREYQLA
                     GQDKLLLKCHCPTLKEKLAAAGALFDYLAA"
BASE COUNT          591 a         1101 c         1280 g          667 t
ORIGIN      
        1 gtagtgaata tccgaggctc gcgagcgaat tcgacacctt gctcgagagc ctgctcatct
       61 tcgttggtat gaccgcgatt tcgggtgcaa gtttctacct gctcgcacaa gagcactcgg
      121 cctggttctg ggcgcaattg ggcgatgaac agcgtgtcgg cgacgtcgtc ggccagtgcc
      181 ttgagccgcc gcgcgtagtc gagcgcagcc ttcgggtcgt gaatcgagca ggggccgaca
      241 acgacaaaga ggcggtcgtc ctcgccgtcg aggatgcggc ggatggtgtc gcggccattg
      301 ctgacggtcg cttcggcgtc gaccgtgatc ggcagtcggg catggatcgc ctgcggcgag
      361 ggcatttcat cgaaggcggc gacgttgatg ttgtcgaggt tctgctgggt catggtcgtg
      421 gggtcttggt tgcgtcttga aatcgttgtc gccgatcagg cggcgaggta gtcgaacagt
      481 gcgccggcag ccgccagttt ttccttgagc gtcgggcagt ggcacttgag cagaagttta
      541 tcctgtccgg ccagctggta ttcccggcgg gtctgcacca gcttgatgat tttcatcgga
      601 tcgagcggcg gcttgggcga gaactgcacc aacagccggt cgatgccggc gtcgagcttg
      661 acgatgccga aggtgccgct gcggatgcgg atgcgatgcg actcgatcag acagcgtgcc
      721 tgtggcggca gttcgccgaa acggtcgatc aattcctctt ggacgtcgcg cagtgactcg
      781 tcatcggtgc aatgcgcgag gcgcttgtag aggttgaggc gctcgtggac gtcggcgcag
      841 tagatgtcgg gaagcagcgc cggttcgtgc aggttgatct cggtgccgat cgccgagggc
      901 agggtcagat cgggctcgtt gccggccttc agtgcggcga ctgcgcggtt aagcatctcc
      961 gaatagagcg cgaagccgac cgcctccatc tcgcccgact ggctgtcgcc aagcacctcg
     1021 ccggcgccgc ggatttccag gtcgtgcatc gccaggtaaa agccggaacc gagttcctcc
     1081 atcatctgga ttgcctcgag ccgttgccgc gcctgcttgt tcacgccctg ttcgtcatcg
     1141 acgagcaggt aggcataggc ctgatgatgc gaacgcccta cgcggccgcg cagctggtgc
     1201 agctgggcga ggccgaactt gtctgcgcgg tcaatcagaa tggtattggc attggggata
     1261 tctatgccag tctcgacgat ggtcgtgcac aacagcacgt tggcgcggcc gtgcgtgaac
     1321 tcgcgcatga ctcgttcgag ctcgacgccg cccatctgcc cgtggccgac cgcaatacgg
     1381 gcctcgggaa cgatgcgcgc aaggcgttcg cgcatgtttt cgatcgtcgc gacttcgttg
     1441 tgcaggaagt acacttggcc gccgcgcttg agttcgcgca ggatcgcctc gcgaatcgtg
     1501 ccctcggcga gccgcgtgac gaaggtcttg atcgacagcc gtttctgtgg tgccgtggcg
     1561 atcaccgaaa aatcgcgtat gccttctagg ctcatcgcca gcgtgcgcgg gatcggtgtc
     1621 gccgtcagcg tcaggacgtc gacttcggca cgcagcctgc gcagcgactc cttctggcgg
     1681 acgccgaagc gatgctcctc gtcgatgacg accaggccga gcttggcaaa acgcacgtcg
     1741 ctctggatca gcttgtgcgt gccgatgacg atgtcgatct tgcccgcggc gagtccggcg
     1801 agcgcctcgg tgctctcctt ggcactgcgg aaacgcgaca gctcggcgat gttgaccggc
     1861 cagccggcga agcggtccga aaaggtgcgg aaatgctgtt cgcagagcag tgtcgtcggg
     1921 cagagcacgg caacctgatg gccgcctgcg accgcggcaa aggcggcgcg cagcgcgacc
     1981 tcggtcttgc cgaagccgac gtcaccgcaa accagcctgt ccattggctt gcccgagcgc
     2041 atgtcggcaa ggacggcggc gatcgccgcc tgctggtcgg gtgtctcctc gaagccgaag
     2101 ccttcggcga aggcctcgta gtcgttcaga gaaaactcga aggcgtgtcc ctggcgggcg
     2161 ttgcgcgctg catacagcgc cagcagctcg gcagcgacat cgcgcgcctg cagcgccgcc
     2221 ttgcgcttgg ccttctccca ctgtgggctg ccaagcgcgt gcagcggcgc cgtgtcgggg
     2281 tcggctccgg tatagcgcga gagcacggcg agctgcgcga ccggaacgta gagcttggct
     2341 tcgccggcgt agtggagttc gatgaactcc atgtcgcctt cgccaagatc gaggtggagc
     2401 aggccctggt agcggccgat gccgtgcgcc tcgtggacga ccggatcgcc gaccttgagt
     2461 tcggtgagat cgcgcagcca gttctcgctg ctcatccgcc ggcgcgaatc gcgtgtgccg
     2521 cgcgaggcgg tgccggggaa caggtcggct tcggcaacga ccgccaactc gccccagatg
     2581 aagccgctct gcagcggcgc tacggtcagc tgggtcgcgc cggcctgcag cgtgatccgg
     2641 cccggtgcgt cgccatcggt ggcgtcgggg gcgccgacgg tttcgatagc cgctatccgc
     2701 tggtcgcgta gcagctcggc aatcgattcc cggcggcccg gcgattcggc ggcgatcagg
     2761 atcgcgccgg cgaaatcagc gcggaacgca cgcaggcgat gggtcgggtc gtctgcacgg
     2821 cggtcgattg ccagctccgg cagtggccgg gttggcatct cgcccgcggg atcgccagcg
     2881 gccccgggcg gcggcgcaat cgaaacacgg gctcggtcgc ggatttgccc gaacaggctt
     2941 tcctcgtcga gaaagagcag cgccggctcg agcaacggtc gatcgcgatc gtggcgcagc
     3001 agctcgaaac gcgatcgggt ttccttccag aaggtcgcca gggcgccctg gacatcacga
     3061 tgcaggcaaa ccgcggcgtc cgccggcagg tagtcgaaga gtgttgccgt ttgctcgaag
     3121 aacaacggca ggtagtactc gatgccggcg gtggcgatgc cactgctgac atcgcggtag
     3181 atcgtggtct tggtcgggtc gttgacgaag tgctcgcgga atcgctggcg gaaggacgat
     3241 cgcccgtcct cgtcgagcgg aaactcccgc gccggcagaa gccggatttc gcccaccggg
     3301 tagaggctgc gctgggtatc gacgtcgaag cagcggatct gctcgacttc gtcgtcgaac
     3361 aggtcgatgc ggtagggaat caccgagccc gatggaaaca ggtcgattag gccgccgcgg
     3421 acgcagaact cgccggggct catgacttgc gagacggcgt ggtagccggc ggtcgcgcat
     3481 tgctggcgca gtttcgtcag gtcgagccgg tcgcccttgc ggatcatgaa ggcatgggcg
     3541 gccaggtaac tggccggcgg caggcggacg acggccgtgg tgatcggcgc gatgatcagg
     3601 tcgcagtcgc cctgggtcag cgcgtagagc gtcgcgagg
//
LOCUS       DATDAA010000041         4674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_142362,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000041 DATDAA010000000
VERSION     DATDAA010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142362"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /gene="pheT"
                     /locus_tag="VIS73_01465"
     CDS             complement(<1..1577)
                     /gene="pheT"
                     /locus_tag="VIS73_01465"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY5761856.1"
                     /translation="MKFPESWLRDFVESPLSGEALGGALTMAGLEVEEFERAAPPFDQ
                     VVVARVLEVARHPDADRLNVCQVDTGDGTPRRIVCGAPNVAPGMVVPCALPGAVLPGG
                     LAIKVAKVRGIESSGMLCSAKELGIDDDASGLLPLADTLKIGSSVRDALQLDDAIFTL
                     KLTPNRGDCLSIRGVSREVLAVSKGKWLADDFARKVPVTIDDSREVSLGAIEACPLYC
                     GRVIRGVDAKAPTPAWMKERLKRCGLRSISALVDITNYVMLELGQPLHAFDNALLAGT
                     ISARMARAGETLLLLNGQRVDIEPDVLVIADDDKVVAMAGIMGGEDSGISLETRDVFL
                     ESAFFAPKAVAGRARRYGFSSDASHRFERGVDFEGTHRALERASELILEICGGDAGPA
                     IEARADLPERRPVTLRGTRLRKVLGIAVDDTEVGRYFDRLRLPWQLSEGVFTVTPPSY
                     RFDIEIEEDLIEEVARLHGYDNVPSRPPRASLAMLPETERQRTLARIRRGLVERGFQE
                     VINFAFVEAAWERDFCA"
     gene            complement(1617..2663)
                     /gene="pheS"
                     /locus_tag="VIS73_01470"
     CDS             complement(1617..2663)
                     /gene="pheS"
                     /locus_tag="VIS73_01470"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY5761857.1"
                     /translation="MEKLDQWVTQAQDDFAAAANADALEQAKARYLGKTGHITELLKG
                     LGKLSPDERKTAGAAINLAKSRIEAALQQRRDALKLAALEARLGEEALDISLPGRASP
                     TGGLHPVTRTLERIEALFRSIGFDVADGPEIETDFHNFTALNTPEDHPARSMHDTFYL
                     QDEAGKLVDGILLRTHTSPIQVRYMQAHVARHAQREAMPEIRVIAPGRVYRVDSDATH
                     SPMFHQVEGLWIGEEVSFADLKGVIADFLRRFFESDDLQVRFRPSFFPFTEPSAEIDV
                     AFMSGSLKGRWLEIAGCGMVHPNVLRHVGIDPERYIGFAFGMGPDRLTMLRYGVNDLR
                     LFFENDLRFLRQFA"
     gene            complement(2790..3149)
                     /gene="rplT"
                     /locus_tag="VIS73_01475"
     CDS             complement(2790..3149)
                     /gene="rplT"
                     /locus_tag="VIS73_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY5761858.1"
                     /translation="MPRVKRGVTARARHKKILKLAKGYRGRRNNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRTLWIARINAAARELGMTYSVFMNGLKKANIEVDRKVLADLAVFD
                     KAAFAQIATQAKAGLGA"
     gene            complement(3161..3358)
                     /gene="rpmI"
                     /locus_tag="VIS73_01480"
     CDS             complement(3161..3358)
                     /gene="rpmI"
                     /locus_tag="VIS73_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY5761859.1"
                     /translation="MPKMKTKSGAAKRFKIRSSGAIKRSQAFKRHILTKKTTKAKRQL
                     RGMTGVHERDAASVRAMMPYA"
     gene            complement(3473..3991)
                     /gene="infC"
                     /locus_tag="VIS73_01485"
     CDS             complement(3473..3991)
                     /gene="infC"
                     /locus_tag="VIS73_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY5761860.1"
                     /translation="MALQKSHRINGEINVPEIRLIGEAGEPLGVVNLSVARNMAEEAG
                     LDLVEIAPTAQPPVCRILDYGKFRYQEAKKAHEAKLKQKQIQLKEVKLRPGTDENDYQ
                     IKLRNLVRFLGEGDKAKITLRFRGREMSHQEFGLRQLERLKADLEEIGQVEQMPKMEG
                     RQMIMIIAPKKK"
     gene            complement(4018..>4674)
                     /locus_tag="VIS73_01490"
     CDS             complement(4018..>4674)
                     /locus_tag="VIS73_01490"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY5761861.1"
                     /translation="LRPDKRVGSDEVWDKAEVALREALRVSGLEWGELPGEGAFYGPK
                     IEFHIKDAIGRSWQCGTMQVDFSMPQRLGAEYVGEDNARHTPVMLHRAILGSLERFIG
                     ILIEDHAGALPLWLAPVQAVVLNISEKQADYAEEVVETLKKAGIRAEADLRNEKITYK
                     IREHSLNRLPYQLVVGDKEKAAGLVAVRTRGGQDLGQMSVVDLIARLGNEIASRVGTA
                     "
BASE COUNT          824 a         1532 c         1400 g          918 t
ORIGIN      
        1 ttggcgcaaa aatcgcgttc ccaggcggcc tcgacgaaag cgaaattgat gacttcctgg
       61 aacccgcgct ccaccagacc gcggcggatg cgcgctagcg tcctctgcct ttcggtctcc
      121 ggcagcatcg ccagcgacgc acgcggcggg cgcgacggca cgttgtcata cccgtgcagg
      181 cgcgccactt cctcgatgag gtcttcctcg atctcgatgt cgaagcgata cgacggcggg
      241 gtgaccgtga acacgccttc actcagttgc cagggcaagc gcagccgatc gaaatagcgc
      301 cccacctcgg tgtcgtcgac ggcaatgccg aggaccttgc gcagacgcgt gccacgcagc
      361 gtgaccggcc ttcgctcggg cagatcggca cgcgcttcga tcgccggccc ggcgtcgcca
      421 ccgcagattt cgagtatcag ttcgctggcc cgctcaagcg cacgatgcgt gccctcgaaa
      481 tcgaccccac gctcaaaacg atgcgacgcg tcgctgctaa aaccatagcg gcgcgcgcgg
      541 ccggcaaccg ctttgggggc gaagaacgcc gattcgagga agacgtcgcg agtctcaagc
      601 gagatgccgc tgtcctcacc gcccatgatg ccggccatcg ccacgacctt gtcgtcgtcg
      661 gcaatcacca gcacgtccgg ttcgatgtcg acgcgctgac cgttgagcag aagcagcgtt
      721 tcgccggcgc gcgccatgcg ggcgctgatc gttccggcga gcagcgcatt gtcgaaggcg
      781 tgtagcggtt ggccgagttc gagcattacg tagttcgtga tatcgaccaa agccgaaatc
      841 gagcgcaagc cgcaacgctt gagccgctcc ttcatccacg ccggcgtcgg cgccttcgcg
      901 tcgaccccgc ggatgacgcg accgcaatac agcgggcagg cctcgatcgc gccgagactg
      961 acttcgcgcg aatcgtcgat ggtcaccggc accttgcgcg cgaagtcgtc ggcgagccac
     1021 ttgcccttgc tgaccgccag cacttcacgg gagacaccgc gaatgctcag gcagtcgccg
     1081 cgatttggcg tgagcttgag cgtgaagatc gcgtcgtcga gctgcaacgc atcacggacc
     1141 gaactgccga tcttcagcgt atcggccaat ggcagcagac cactcgcatc atcatcgatg
     1201 ccgagttcct tggccgagca gagcatgccc gacgattcga tgccacgaac cttggcgacc
     1261 ttgatcgcca ggccgccggg cagcactgca cccgggagcg cacagggcac gaccatgccc
     1321 ggcgcgacgt taggcgctcc gcagacgatg cggcgtggcg tgccgtcacc ggtatcgacc
     1381 tgacagacat tcaggcgatc ggcatcggga tgcctggcaa cctcaagcac gcgcgcgacg
     1441 acgacctggt cgaaaggcgg tgcagcgcgc tcgaactcct cgacttcaag cccggccatg
     1501 gtcagggcgc caccgagcgc ctcgccggag agtggtgatt cgacgaaatc gcgcagccag
     1561 gattccggaa atttcatctt ctttcctctt cgtcccgcct cgcgggaccg attcgatcag
     1621 gcaaactgcc gcaggaaacg caggtcgttt tcgaagaaca ggcgcaggtc gttgacgcca
     1681 tagcgcaaca tggtcaggcg gtcgggaccc atgccgaagg caaagccgat gtagcgctcg
     1741 ggatcaatac cgacatggcg cagcacgttc ggatgcacca ttccgcaacc ggcgatttcc
     1801 agccagcggc ccttgagcga accgctcatg aaggccacgt cgatctcggc cgagggctcg
     1861 gtaaacggga agaacgaggg gcggaaacgc acctgcagat cgtccgattc gaagaagcgg
     1921 cgcaggaaat ctgcgatcac gcccttaagg tcggcaaagc tcacttcctc gccgatccac
     1981 agcccttcga cctgatggaa cattggcgaa tgcgtcgcgt cggagtcgac gcgatagacg
     2041 cggcccggcg cgatgacgcg gatctcgggc atcgcctcgc gctgcgcgtg ccttgccaca
     2101 tgcgcctgca tgtagcgcac ctggatcggg ctcgtatggg tgcgcagcag gatgccgtcg
     2161 acgagcttgc cggcctcgtc ctgcagatag aaggtgtcgt gcatcgaacg cgccgggtga
     2221 tcctcgggcg tattcaatgc cgtgaagttg tggaagtcgg tttcgatctc gggaccgtcg
     2281 gcgacgtcga aaccgatcga gcggaacagt gcttcgattc gttcgagcgt gcgagtcacc
     2341 ggatgcaggc ctccggttgg actggcgcga cccggcaggc tgatatccag cgcttcctcg
     2401 ccgagacgag cctccagggc tgccagcttc agcgcgtcgc gacgctgctg gagcgcagct
     2461 tcgatgcgac tcttggctag gttgatcgcg gcgccggcgg tcttgcgttc gtcgggcgag
     2521 agcttgccca gccccttgag caactcggtg atatggccgg tcttgcccag ataacgcgcc
     2581 ttggcctgct cgagcgcatc ggcattggcg gccgcagcga agtcatcctg cgcctgcgtc
     2641 acccactggt cgagcttctc catcctgccc cctcagaacc gccgacccgg tcggctttcc
     2701 cgtactgcca ttccttgcac gccgaccacg agcgccggca ctagaaaaaa aaggaggccg
     2761 gggcctcctt ttccgttcgc ggcaatcgct caggcgccga ggccggcttt cgcctgcgtg
     2821 gcgatctgcg caaacgccgc cttgtcgaag acggcgaggt cggcgaggac cttgcgatcg
     2881 acttcgatgt tggccttctt cagaccgttc atgaacacgc tgtaggtcat tcccagttcg
     2941 cgcgcagcgg cattgatacg ggcgatccac agggtgcgga actgacgctt tctctgccgg
     3001 cggtcacgat acgcatattg accggccttc atcaccgcct gtttggcgac gcgatagacg
     3061 ttgttgcggc ggccgcggta acccttggcc agcttgagaa ttttcttgtg gcgcgcgcgc
     3121 gccgttacac cacgtttgac tcgaggcatc gctgttctcc ttacgcgtag ggcatcatgg
     3181 cgcggacgga tgcggcgtcg cgctcatgaa caccggtcat gccgcgcagt tgacgcttgg
     3241 ccttggtggt cttcttggtc aggatgtggc gcttaaacgc ctgcgagcgc ttgatcgcgc
     3301 cgctcgagcg gatcttgaac cgcttggcgg ccccgctctt ggtcttcatt ttcggcatgc
     3361 ttctactccg atgaatgtca tgtcgtcagg tgtcttccca cggaagcgct ttcctacccg
     3421 acgccacttg ttatggccgg ccgtctcgcg acagcctgcc gaattccccg tgctacttct
     3481 tcttcggcgc aatgatcatg atcatctggc gcccttccat cttcggcatc tgctcgacct
     3541 ggccaatctc ttccaagtct gccttcaagc gctccaactg tcgcagcccg aactcctggt
     3601 ggctcatctc gcggccccgg aagcgcagcg tgatcttcgc cttgtcgccc tcgcccagaa
     3661 agcgcaccag attgcgcagc ttgatctggt agtcgttctc gtcggtaccc ggccgcagct
     3721 tcacctcctt gagctgaatc tgcttctgct tgagcttcgc ctcgtgagcc ttcttggctt
     3781 cctggtaacg aaacttgccg taatcgagga ttcggcagac cggcggctgg gccgttggcg
     3841 cgatttcgac cagatcgagt ccggcttctt cggccatgtt gcgggccacg ctgaggttaa
     3901 cgacgccgag tggttctccg gcttctccga tcaggcgaat ctccgggacg ttgatctctc
     3961 cgttgatccg atgcgacttt tgcagtgcga tggttaactc tccagaattg acaaaaacta
     4021 agccgtgcca acacgggacg cgatctcgtt tccgagtcgc gcaatcaggt ccacgacgct
     4081 catctgtccg aggtcctgac cgccacgtgt gcgcacggcc accaagccgg ctgccttttc
     4141 cttatcccct acaaccagct gatagggcag tcggttcaag ctatgttcgc ggattttata
     4201 cgtaattttc tcgtttcgca aatccgcttc ggcccgtatt ccggcctttt taagcgtttc
     4261 gacgacttcc tcggcgtaat cggcctgctt ctccgaaatg ttcaggacaa cggcctgaac
     4321 cggcgccagc cagagcggca gcgcgccagc gtggtcttcg atgaggatgc cgatgaagcg
     4381 ctcgagcgaa ccgaggatcg cgcggtgcag catgactggt gtgtggcggg cgttatcctc
     4441 gcccacgtat tcggcgccga gtcgctgcgg catagaaaaa tcgacctgca tcgtgccgca
     4501 ctgccatgaa cgaccgatcg cgtccttgat gtggaattcg atcttcggcc cgtagaaggc
     4561 gccctctccg ggcagttcac cccactccag gccagatacc cgcagcgcct cccgcagcgc
     4621 tacctccgcc ttgtcccaga cttcgtcgga accgacccgc ttgtccggcc gcag
//
LOCUS       DATDAA010000042         3333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_3119,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000042 DATDAA010000000
VERSION     DATDAA010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3119"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..946)
                     /locus_tag="VIS73_01495"
                     /pseudo
     CDS             complement(1..946)
                     /locus_tag="VIS73_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
     gene            complement(949..1470)
                     /locus_tag="VIS73_01500"
     CDS             complement(949..1470)
                     /locus_tag="VIS73_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761862.1"
                     /translation="MRHYPQNSPEAVSRIVALALVADGRLEATELDMLTRSGAFGRIG
                     VSEERFLQVLKEFCADLLDHRREPSGDECQLGRDDVAQLLGDLDNPERQRAVLRIMLD
                     VIRADGRLHEGESMLVWQALDQWRIRLTDIVRPAAKRVTRYPRIPRGIRRKKPKPQAQ
                     APQFGRGFAGQGA"
     gene            1589..2503
                     /locus_tag="VIS73_01505"
     CDS             1589..2503
                     /locus_tag="VIS73_01505"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5761863.1"
                     /translation="MNRRHITFRQLEIFAAVARQKSFTRAAELLHLTQPAVSLQMQKL
                     AEGIGQPIVEVIGREVVLTPVGRALLETTAGIDDAWDRFAATVEAHGALQHGTLRVAT
                     VTTAKYFLPRMLGRFSHEHPGIDIELEVLNRERIIARLRDGLDDLYVMGVPPEEIKVV
                     SDPFLDNELVVIAPADWKPPRHGRLQLADLAGERFILRETGSGTRRLIDNHLARAGQR
                     LVVKLVLGSNEAIREAVAGGMGLAILSRHALPAGLAGGDIRIVDVEGFPLRGRWYVVH
                     RADRRLSRPAEAFRTALLGEAGARCESV"
     gene            2562..3023
                     /gene="ssb"
                     /locus_tag="VIS73_01510"
     CDS             2562..3023
                     /gene="ssb"
                     /locus_tag="VIS73_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY5761864.1"
                     /translation="MASVNKVILVGNLGADPETRYMTNGDAVCNIRVATTESWKDKGT
                     GEKKELTEWHRVVFYRKLAEIAGQYLKKGSAVYLEGRIRTRKWTDKEGVERYTTEIEA
                     NEMQMLGKREGQGSPAGRDSGGGDFRPSPQGSGGGKPAKADFDNFEDDIPF"
BASE COUNT          575 a         1074 c         1116 g          568 t
ORIGIN      
        1 ccatcaggaa gtggccgagg tggcgcagca gcattacgcc cgagagaatg ccagcggtcg
       61 ggttggccgt gtcccgtccg gcatgtcccg gcgccgagcc gtggactgcc tcgaatctgg
      121 cgatgccttc gccgatgttg gccgagggcg cgaagccgag cccgccgacc agaccggagg
      181 tgaggtcgga aaggatgtcg ccgttcatgt tggtggtgac gattacctcg aactgctcgg
      241 gatagcgcac gagctggtgc gcgcagttgt cgaccagcaa atgcgcggcg gtgatgtcgg
      301 gatagtcgcg cgcgacatcc tcgaaggcgc gcttcatcag gccctcggtg agtttcatga
      361 tgttggcctt ggtcgcgcag tgcaccgtgc gacgtccttc ggcgcgcgcc agctcgaagg
      421 ccagccgcgc gatccgcgcg cagccctgat aggaaaccag cttcaggcac tgggcgacgt
      481 tgggcgtttg ttgatgctcg atgccggcgt agagatcctc gacgttctcg cggacgacga
      541 ccaggtcgat gttacgtccc gcgtacggcc cgccgacgcc cgaatactcg cgcaccggac
      601 gaatgttgcc gaaggtctcg aagagcttgc gcagggtcac attggcgctc ttgccgccct
      661 agccgatggg cgttgccagt ggccccttga ggacgattcg attcttcgcg atcgaatcga
      721 tggtctcgcg cggcacgccg ctggcgatgc cggcggcgaa gaccgcctcg cccgcatcag
      781 cgatttccca gtcgatcggc gcttcgaccg cggcgaatac ccgctgtacg ccgcgcatga
      841 tttcggggcc gatgccgtcg ccgggaatca gcgtcgccgt ggtccgcgtc atcgcctcgg
      901 tccgccgggc gctgcggtgc tcggtcagga gggaggtgtc gagcatgatc acgccccctg
      961 accggcgaag ccgcgaccga actgcggtgc ctgcgcctgc ggctttggct tcttgcggcg
     1021 gatgccgcgc gggatgcgcg ggtagcgggt gacgcgcttc gctgccgggc gcacgatgtc
     1081 ggtcagccgg atgcgccact ggtcgagcgc ctgccagacc agcatcgatt cgccttcgtg
     1141 caggcggccg tcggcacgga tcacgtcgag catgatgcgc agtacggcgc gctggcgctc
     1201 cgggttgtcg aggtcgccca ggagctgcgc gacatcgtcg cggccgagct ggcactcatc
     1261 gccgctcggc tcccgccggt ggtcgagcag gtcggcgcag aactccttaa gcacctgcag
     1321 gaagcgctcc tcgctgacgc cgattcggcc gaatgcgcca cttctggtca gcatgtcgag
     1381 ttccgtcgct tcgagacggc catcggcgac cagtgccagc gcaacgattc gtgaaaccgc
     1441 ttccggactg ttctgtgggt agtgacgcat ggttcggttc tccttggttg gcgcgacgca
     1501 gaacggcggt cgctgcaact tcgtccgttt gtccaactca taaattccat atgaaccatt
     1561 gggctttgtc ataagacaaa gcatatgcat gaaccgccgt cacatcacct ttcgccagct
     1621 cgaaatattt gccgccgtgg cgcggcagaa gagctttacg cgtgccgctg aactgctgca
     1681 tctgacgcag ccggcggtgt ccctgcagat gcagaagctg gccgagggaa tcggccagcc
     1741 gattgtcgag gtgatcggcc gcgaggtcgt gctgacgccg gtaggtcgcg ccctgctcga
     1801 aaccacggcg ggaatcgacg acgcctggga tcgcttcgcc gccacggttg aggcgcacgg
     1861 cgcgttacag cacggcacgc tacgcgtcgc cacggtgacc acagccaagt atttcctgcc
     1921 gcgcatgctc ggccgcttca gccacgagca tccgggaatc gacatcgaac tcgaggtgct
     1981 gaaccgcgag cggatcatcg cccgcctgcg cgacggcctc gacgatctct atgtgatggg
     2041 cgtgccgccc gaggagatca aggtcgtcag cgatcccttt cttgacaacg agctggttgt
     2101 gatcgctccc gccgactgga agccgccacg gcacggtcgc ctgcaattgg ccgacctcgc
     2161 cggcgagcgc ttcatcctgc gcgaaaccgg ttcgggcaca cggcgcctga tcgacaatca
     2221 cctcgcccga gccggccagc ggctggtcgt caagctggtg ctgggcagca acgaggcgat
     2281 ccgcgaggcc gtcgccggcg gcatgggtct ggcgattctt tcgcgccatg ccctaccggc
     2341 cgggctggcg ggcggcgaca tccgcatcgt cgatgtcgag ggcttcccgc tgcgcggccg
     2401 ctggtacgtc gtgcaccgcg ccgaccggcg gctctctcgc ccggccgagg cgtttcgcac
     2461 ggcactgctc ggtgaggcag gcgcgcgatg cgaatccgta taatgcgcgc tcggcggcgg
     2521 gcgtctcccg cgcgccgcga gccaaaaagg gaggagaacg catggcctcg gtaaacaaag
     2581 tcatcctcgt cggcaacctc ggtgccgacc cggaaacccg ctacatgacc aacggcgatg
     2641 ccgtctgcaa catccgggtc gcgaccacgg agtcgtggaa ggacaagggc accggcgaga
     2701 agaaggagct gaccgaatgg caccgcgtgg tcttctatcg caagctcgcc gaaatcgccg
     2761 ggcagtattt gaagaagggg tcggcggtct atctcgaagg ccgcatccgc acgcgcaagt
     2821 ggaccgacaa ggaaggcgtc gagcgctaca ccaccgagat cgaagccaac gaaatgcaga
     2881 tgctcggcaa gcgtgaaggc cagggctcac cggccggccg cgacagtggc ggtggcgact
     2941 tccgtcccag tccgcagggc agtggcggcg gcaagccggc gaaggccgac ttcgacaact
     3001 tcgaggacga cattcccttc tgaattacac ctagtacctg taaagatcca ttcagcctct
     3061 tcggaggctg ttttcttttc tggcgccgga tcgacggcga gccgggcagg tcaattccga
     3121 attccggcgg aactcattgc cgcggcaccg cctaaactga gacggaaact ggaggcgtca
     3181 tgggctggaa aaacacccga gcggagacag gcatcagcaa gttagagcga gcgaccgcac
     3241 atggcctttt cagcgctggc catggcgcgc ggctcgcatc gcccagcgtc accggccagc
     3301 ccggggacgg cgggcaaccg gggcgctgcg cgt
//
LOCUS       DATDAA010000043         2801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_457370,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000043 DATDAA010000000
VERSION     DATDAA010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457370"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VIS73_01515"
     CDS             complement(<1..216)
                     /locus_tag="VIS73_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761865.1"
                     /translation="MSVRFNSLVRPWRRLLLAPVLSVAAVVGHAEVINVDNAEFQRLL
                     EQGVTVVDLRTAPEWKQTGVIDRSHLIT"
     gene            complement(347..2638)
                     /locus_tag="VIS73_01520"
     CDS             complement(347..2638)
                     /locus_tag="VIS73_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEY5761866.1"
                     /translation="MSFRREDALAYHAAGRPGKIEVNPTKPFKAQRDLALAYSPGVAE
                     PCKEIAAHPEDVYRYTARGNLVAVVSNGTAVLGLGNIGALAGKPVMEGKGVLFKTFAD
                     IDVFDLEVDISDAEQLVEIVASLEPTFGGINLEDIKAPECFYIERKLRERMNIPVFHD
                     DQHGTAIVVGAAVLNGLHYLGKDLKKVKVVTSGAGAAALACLDLLVMLGVPVENIWVS
                     DIKGVVYQGRVEDMDEIKGRYAKQTDARTLGEIIADADVFLGLSAGGVLKAEMAAKMA
                     AKPLILALANPTPEILPEEVQRVRDDAIICTGRSDYPNQVNNVLCFPFIFRGALDVGA
                     TTITEEMKLAAVKAIADLARAEQSDIVAAAYGEKVSAFGPEYLIPKPFDPRLIAKIAP
                     AVARAGMDSGVATRPITDWAAYAQSLNEFVYHSGLIMKPVYAAAKAAPLKRIVFCEGE
                     DERVLRAIQVVCDEGIARPVLIGRADEIARKIEAFGLRVQPERDFEVIHHEHSAFFDT
                     HFEALWKTYYDLMQRRGVSIDYAKREVRRRATLFGSLMVRSGLADGMICGLFGRHEAH
                     RSYVQSVLGTAAGASRYYTMNLLMLPERTVFIADTYVNYDPSAEQLTEMTLLAAREIR
                     RFGVQPKVALLSHSTFGSEDTPTSLKMREVLRMLSERAPDLEVEGEMHGDAALDENIR
                     RQVFPNARLKGQANLLIMPTLDAANISFNLLKTAAGDNVTVGPILLGAAQPVHVLTPT
                     ATVRRIVNMTALTVVDAAAGEGR"
BASE COUNT          511 a          904 c          892 g          494 t
ORIGIN      
        1 cgtgatcagg tgactgcggt cgatgacgcc ggtctgcttc cactcgggcg ccgtgcgcag
       61 gtcgacgacc gtgaccccct gttccaacag gcgctggaat tccgcgttgt cgacgttgat
      121 gacttcggcg tggccgacca ccgccgcgac cgagaggaca ggggccagca acaggcgacg
      181 ccatggcctg acaagcgaat tgaatcgaac cgacatgggt gtctccgcga caacggggtg
      241 accaccgagt ctataggtcg ccgacaccgg catcggcgac tgcagtcaca ctccgaacct
      301 cagcaaagac aacagcgctg caccggtgtt cccggcaagc gcgcgttcag cgcccttcac
      361 cagctgccgc gtcgacaacg gtcagcgcgg tcatgttgac gatgcgacga accgtcgccg
      421 ttggcgtcag cacatgcacc ggctgggccg ccccgagcag aatcggaccg acggtaacgt
      481 tgtcgccggc agcggtcttt agcaggttga aggagatgtt ggccgcgtcg agcgtcggca
      541 tgatcaacag atttgcctgc cccttgagcc tggcgttggg gaagacctgg cgccggatat
      601 tttcgtccag cgccgcgtcg ccgtgcatct cgccctcgac ttcgagatcg ggagcccgct
      661 cgctcagcat gcgcagaact tcacgcatct tcagcgacgt cggcgtgtcc tcgctgccga
      721 aggtcgaatg cgacagcaga gcgaccttgg gttgaacgcc gaaccggcgg atctcgcgtg
      781 cggccagcag cgtcatctcg gtgagttgtt cggcggaggg atcgtagttg acgtaggtat
      841 cggcgataaa caccgtgcgc tcggggagca tcaacaggtt catcgtgtag tagcggctgg
      901 caccagcggc agtgccgagc acgctctgga cgtaggagcg atgcgcctcg tggcgtccga
      961 acagcccgca gatcatgccg tcggcgaggc cgctacgcac catcagcgag ccgaacagcg
     1021 ttgcccgccg gcgcacttcg cgcttggcgt agtcaatcga taccccgcgc cgctgcatca
     1081 ggtcgtaata ggttttccag agcgcctcga aatgggtatc gaagaacgcc gaatgctcgt
     1141 ggtgaatgac ctcgaagtct cgctcgggct gcacacgcag gccgaaggcc tctatcttgc
     1201 gggcgatttc atcggcgcgc ccgatcagga ccggacgggc aattccctca tcgcagacca
     1261 cctggatagc gcgcagaacg cgctcgtcct cgccttcgca aaaaacaatt cgcttcaaag
     1321 gcgcagcctt cgctgccgcg tacaccggct tcatgattag gccggagtga tagacgaact
     1381 cattgaggct ctgcgcgtag gcagcccagt cggtgatcgg gcgagtcgcc acccccgagt
     1441 ccataccggc gcgggcaacg gccggcgcaa tcttggcgat cagccttgga tcgaagggct
     1501 tcgggatcag gtactccggg ccaaaggccg agactttctc gccgtaggcc gccgccacga
     1561 tgtccgactg ctcggcgcgc gccagatcgg caatggcctt gaccgcggcc agcttcattt
     1621 cttcggtaat cgtagtcgcc ccgacatcca gcgcgccgcg aaaaatgaac gggaagcaca
     1681 gcacgttgtt gacctggttc gggtaatccg agcggccggt gcagatgatc gcatcatcgc
     1741 ggacgcgctg tacttcctcg ggaagaatct cgggcgtcgg attggccagc gccagaatca
     1801 gcggcttcgc tgccatcttt gcagccatct cggccttgag aacaccgccg gccgacaacc
     1861 cgagaaagac atcggcgtcg gcgatgattt cgccgagcgt ccgcgcgtcg gtctgcttgg
     1921 cgtagcgccc cttgatctcg tccatgtcct cgacgcgccc ctgatagacg acgcccttga
     1981 tgtccgaaac ccagatgttc tcgaccggca caccgagcat tacgagcaga tcgaggcaag
     2041 ccagcgccgc cgcgccggcg cccgaagtga cgaccttgac cttcttcagg tccttgccaa
     2101 gataatgcaa accgttaagg accgccgcgc cgacgacgat cgccgtacca tgctggtcgt
     2161 cgtgaaacac cgggatgttc atccgctcgc gcagcttccg ctcgatgtag aagcactcgg
     2221 gcgccttgat gtcctcgagg ttgatcccgc cgaaggtggg ctcgaggctg gccacgattt
     2281 cgacgagctg ttcggcgtcg gatatatcga cttcgagatc gaagacgtcg atgtcggcga
     2341 acgtcttgaa cagaacaccc ttgccctcca tgaccggctt gcccgccagt gcgccgatat
     2401 tgccaagtcc gagcaccgcg gtgccgttgg acacgacggc aaccaggttg ccccgggcgg
     2461 tgtatcggta cacatcctcg gggtgggccg cgatctcctt gcagggttcg gccacgcccg
     2521 gcgagtacgc gagggccagg tcccgttgcg ccttgaacgg cttggtcggg ttgacttcga
     2581 tcttgcccgg gcgcccggcg gcgtgatacg cgagtgcgtc ttcccggcgg aaggacatac
     2641 gcaagtgcct gaaaatgaga gagtgaggct agcgcgcccc cgcgctggtg tcaagcgaac
     2701 ccccgggccg gggctgaccg ggcggcgggt cgccggcatc gcgcgtctgc tggtggccgc
     2761 ggcgtgcgga gaccccaccg gccccgaggg cgatcgcctc g
//
LOCUS       DATDAA010000044        10209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_108322,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000044 DATDAA010000000
VERSION     DATDAA010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108322"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VIS73_01525"
     CDS             <1..316
                     /locus_tag="VIS73_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY5761867.1"
                     /translation="PLPPGVVVRCRPVGMLGMEDEAGGDAKVLAVPIDKLTTMYRDVD
                     SPRDLPQIVLDQISHFFEHYKDLEPGKFVRIKGWFGPEEAKQEIMDGVKRYNSSKDKP
                     AF"
     gene            358..1971
                     /locus_tag="VIS73_01530"
     CDS             358..1971
                     /locus_tag="VIS73_01530"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY5761868.1"
                     /translation="MTLRVAIAQINATVGDLDGNVRRLIAAAKTARAEGADLLLTPEL
                     AISGYPPEDLLLRQDFYRACGAALEHLARASVGIDIVVGHPFIDGERRYNAATLLRDG
                     AVTNIGYRKARLPNYEVFDEARYFVAGDTACVFELKGRRLGVNICADVWDPGAAEAAH
                     AAGAEILLVLNASPYHVGKEAERLEVLRERIAATGMPIVYANLVGGQDELVFDGASMV
                     LDASGNLRAQLRRFEEATEVFSIDGDVVSGPLYAPFDSPEAEIYQALVTGVCDYLGKN
                     NFPGAIIGLSGGIDSALTLCVAVEALGASRVRAVMMPSPYTADISIDDSQALVKRLGV
                     AYDEIPIGAAMKTYDEMLAPFFAGLAADTTEENVQARVRGNLLMALSNKTGSLVLTTG
                     NKSEMAVGYCTLYGDMAGGFAVIKDIPKTMVYRLSRWINRNGEVIPDRIITRPPSAEL
                     RPGQTDQDSLPPYDVLDAIVEAYMERDESPRQIIAAGYGEADVRRVVRLLKISEYKRR
                     QAPLGVRISQRGFGKDWRYPITNRYADEW"
     gene            2036..2374
                     /locus_tag="VIS73_01535"
     CDS             2036..2374
                     /locus_tag="VIS73_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY5761869.1"
                     /translation="MKKIEAIIKPFKLDEVREALSEIGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKMKIELVVADATVDQAIEAIIKAARTGKIGDGKIFVMPVDQVVRI
                     RTGETDEAAV"
     gene            2616..3326
                     /locus_tag="VIS73_01540"
     CDS             2616..3326
                     /locus_tag="VIS73_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5761870.1"
                     /translation="MDSRSLIRPVLAAIFAGIVCIPTAFAAETESAVARGGRLYDKFW
                     AENKGAKPEADHAAYPHKGGKYGKDASWRCKECHGWDYMGKDGAYSSGGHATGIKGIR
                     AAAGKDVAAIVAVLKDKTHGYTDQHLSAKDFNDVALFVSKGQVDMDKYIDRAGKKSKG
                     DAGKGEVYFNTICAGCHGTDGKKIKDADPLAAAAGNPWELLHKVLNGHPGESMPALRA
                     LDPQIAADLVAHMQTLPK"
     gene            3420..3989
                     /locus_tag="VIS73_01545"
     CDS             3420..3989
                     /locus_tag="VIS73_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEY5761871.1"
                     /translation="MIKRTWAWLRRPAANLSAGVLLVLGGLGGILFWGGFNTAMEYTN
                     RLEFCVSCHEMNQLVHGEYRESVHYSNASGVRAICSDCHVPRPWGAKVWRKIRASGEV
                     WHWLRGTIDTPEKFEAQRLVMAERVWKEMTDNNSRECRNCHDYQAMAFHKQSLRAREK
                     MEEAKDKNKPCIECHKGVAHRKPLVERDD"
     gene            complement(4186..5148)
                     /locus_tag="VIS73_01550"
     CDS             complement(4186..5148)
                     /locus_tag="VIS73_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5761872.1"
                     /translation="MKVASLLGVLALSTLLAQPALAANVKVTPLGGQDGEFCILDRAL
                     VFEDPNGTRVLYDPGRTVAGAADARLGKIDVVLVSHMHGDHVGDKHTKAPNAGACDKP
                     DMSVSALPNSLAVDIALAKNAKIVTGSEMPTFFANKLKAAGGDPKNSVLARFGASQTI
                     GGVTIATVTAIHSNAVDGDLVGGDLGKTLKEAGLGAYVGEATGYVLKFSNGLAVYLSG
                     DTGITGDQEKVVRGHYGAKLAVINIGDTFTTGPNEAAYVINDLVRPASVIVSHANEPA
                     TAGGKVKPGTRTESFIKATKVPVHVPLSGRTMEFDADGKCVSGC"
     gene            complement(5229..5732)
                     /gene="tpx"
                     /locus_tag="VIS73_01555"
     CDS             complement(5229..5732)
                     /gene="tpx"
                     /locus_tag="VIS73_01555"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEY5761873.1"
                     /translation="MASKITHAGNSITVAGDFPKKGQKAPPLSLVAKDLSDATLAHFA
                     GKRKVLNIFPSIDTPTCATSVRKFNEKASALKNTVVLCISADLPFAQGRFCGAEGLAN
                     VVNLSTMRGANFLKDYGVLIDSGLLKGLAARAVVVLDENDTVLDAELVPEIKQEPDYD
                     RALRALG"
     gene            complement(5824..7077)
                     /locus_tag="VIS73_01560"
     CDS             complement(5824..7077)
                     /locus_tag="VIS73_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HEY5761874.1"
                     /translation="MPQLPAHPASFDWTVVAIFAIVYLGMFLGGLPRLKLDRAGIALL
                     GAIAVIGSGAMSPESAAQAVHLPTVLLLFAFMVMSAQMRLGGFYTAVTRAVAALPLAP
                     AAWLAALIAVVAGLSAVFSNDIVCLAVTPVLVRICLQRSLNPLPFLVGLACAANIGSA
                     ATLIGNPQNMLIGAVARLSFVDYIVAALPPVLLSLLALWLWLARAYRPGLLAERQTPV
                     DLAADDAPAFDRWQSAKGLAVAALVMLAFLFTDWPREVVALVAAGVLLLSRRLHSAQV
                     MGFVDWPLLVLFAGLFVVNGAFERTGLAADAVHWLAVHGVVLDAPGPLLVIGVGLSNL
                     VSNVPAVMLLLPHLHSSEAATLLALASTFAGNLLLVGSIANLIVADLARRTGVEIDWK
                     QHARIGIPVTLMSLAVLAGTQALLR"
     gene            complement(7228..8901)
                     /locus_tag="VIS73_01565"
     CDS             complement(7228..8901)
                     /locus_tag="VIS73_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEY5761875.1"
                     /translation="MRRLTQLSTALGLLAMSALAIAGPAVEGAIEKQPTNWHAITMFI
                     VFVALTMGITYWASSRTKSTSDFYTAGGGITGFQNGLAIAGDYMSAATLLGLTAMVFS
                     RGYDGYLYMLAFFAGWPIILFLMAERLRNLGKFTFSDITAYRLDQGKVRTMAAISSLT
                     VVCFYLIAQMVGAGQLIKLLFGLDYTVAIFGVGILMMVYVTFGGMVATTWVQIIKACM
                     LLSGGTLVMVLAMSQFDFSFTTLMEKATSIHSLGDKLMHPGGLLKDPITAISLGLGLM
                     FGTAGLPHILMRFFTVSDAKEARKSVLYASGFVAYFFNVILLMGLAAILIVGQNPEFF
                     EGGDVGGKLVGGGNMVAMHLAKAVGGDMLLGFLAAVAFATILAVVSGLALAGASAISH
                     DLYARVIKKGTASEASEIRVSRIATICLGFVAILLGIAFEKQNVAFMVGLAFGVAAAA
                     NFPVLILSMYWKGLTTRGALAGGYGGLTSAVLLVVLSKSVWVDVFGFDSPIFPYTQPA
                     LFAMPVAFLLAFLVSKLETSARAAQEVAAFEDQYVRAQTGFGAAAAAQH"
     gene            complement(8901..9221)
                     /locus_tag="VIS73_01570"
     CDS             complement(8901..9221)
                     /locus_tag="VIS73_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEY5761876.1"
                     /translation="MSSAMYGRMRANPKFQELVTQRRRFAWTLAAIVLTMFYGFVLTV
                     AFSPATIGKPLAEGSMWTVGVAAELFMFVFFWLLTALYVRRANSHFDKLTDDIVKDAW
                     KENR"
     gene            9525..>10209
                     /locus_tag="VIS73_01575"
     CDS             9525..>10209
                     /locus_tag="VIS73_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5761877.1"
                     /translation="MPADSPATEACLSADRARIGGLARRLAKAQGAEREKVGVQLAKL
                     RASSAAAFARRAESLPRPEFSDELPVNGRRAEIAAAIRDHQVVIVCGETGSGKTTQLP
                     KILLELGIGAAGLIGHTQPRRLAARSVAARLAEELKTSVGRGVGVKIRFHDRTGPDAW
                     VKVMTDGILLAETQGDHQLSAYEAIIIDEAHERSLNIDFLLGYLKTLLARRSDLKLIV
                     TSATIDAERF"
BASE COUNT         1912 a         3190 c         3445 g         1662 t
ORIGIN      
        1 tccgctgccg ccgggcgtgg tcgtgcgctg ccgcccggtc ggcatgctcg ggatggagga
       61 cgaggccggc ggtgacgcca aggtgctcgc cgtgccgatc gacaagctga ccacgatgta
      121 ccgcgatgtc gactcgccgc gcgatctacc gcagatcgtc ctcgaccaga tttcgcactt
      181 cttcgagcac tacaaggacc tcgagccggg caagttcgtc cgaatcaagg gctggttcgg
      241 tcccgaggaa gcgaagcagg aaatcatgga cggtgtgaag cgctacaaca gcagcaagga
      301 caagccggcc ttctgatcga ctgcgcggat tgaaccgatg atgcttgagg cgggcgcgtg
      361 acgctgcgcg ttgcgatcgc gcagatcaac gccacagttg gcgatctcga cggcaacgtc
      421 cgtcgcctga tcgccgctgc caagacggcg cgtgctgaag gcgccgatct gctgctgacg
      481 cccgagttgg caatctccgg ctatccgccc gaggacctgc tgctgcgtca ggacttctac
      541 cgcgcctgcg gtgccgcgct cgaacatttg gcaagggcca gcgtcggcat cgacattgtc
      601 gtcggacatc cgttcatcga cggtgagcgt cgctacaacg ccgcgacgct gctgcgcgac
      661 ggcgcggtca ccaacatcgg ttaccgcaag gcgcgcctgc ccaactacga ggtcttcgac
      721 gaggcacgct atttcgtggc cggcgacact gcctgcgttt tcgagctcaa ggggcgcagg
      781 ctcggcgtga acatctgtgc cgatgtctgg gatccgggcg ccgccgaggc cgcccacgct
      841 gccggcgccg agatcctgct cgtgctcaac gcctcgccct accacgtcgg caaggaagcc
      901 gagcggctcg aagtgctgcg cgagcgtatc gccgcgaccg gaatgcccat cgtctacgcc
      961 aacctcgtcg gcggccagga cgaactggtc ttcgacggcg cgtcgatggt gctcgacgcc
     1021 agcggcaacc tgcgcgccca gctgcgccgc ttcgaggaag cgaccgaggt cttcagtatc
     1081 gacggcgatg tggtctcagg cccgctctac gcgcccttcg attcgcccga agccgagatc
     1141 tatcaggcgc tggtcaccgg cgtctgcgac taccttggca agaacaattt tcccggtgcg
     1201 atcatcggcc tctcgggcgg catcgattcg gcgctgacgc tgtgtgttgc cgtcgaggca
     1261 ctcggtgctt cgcgcgtgcg cgcggtgatg atgccatcgc cctacaccgc cgacatcagc
     1321 atcgacgatt cgcaggcact ggtgaagcgc cttggcgttg cctacgacga gattccgatc
     1381 ggcgcggcga tgaagaccta cgacgaaatg ctcgcgccgt ttttcgccgg gctcgccgcc
     1441 gataccaccg aagaaaacgt ccaggcgcgc gtgcgcggca acctgctgat ggcgctctcg
     1501 aacaagaccg gatcgctggt gctgaccacc ggcaacaagt cggaaatggc cgtcggctac
     1561 tgcacgctct atggcgacat ggccggtggc ttcgcggtga tcaaggacat ccccaaaacg
     1621 atggtgtacc ggctgtctcg ctggatcaac cgcaacggcg aggtgatccc cgaccgcatc
     1681 atcacccgcc cgccctcggc cgagctgcgg cccgggcaga ccgaccagga cagcctgccg
     1741 ccctacgacg tgctcgacgc catcgtcgag gcctacatgg agcgcgacga aagcccgcgg
     1801 cagatcattg ctgccggcta cggcgaggcc gacgtccggc gcgtcgtgcg cctgctgaag
     1861 atatccgaat acaagcgccg tcaggcaccg ctcggcgtgc ggatttccca gcgtggcttc
     1921 ggcaaggact ggcgttaccc gatcaccaac cgctacgctg acgaatggta aggttcgccc
     1981 tgagcggaac cactcattcc agcgccatta gacccatcct taggagacag gcccgatgaa
     2041 aaaaatagaa gcgatcatca aacccttcaa gctcgacgaa gtgcgcgagg cgctttcgga
     2101 aatcggcgtg accggcctga cggtcaccga ggtcaagggc ttcggccggc aaaagggcca
     2161 taccgagctg taccgcggtg ccgagtacgt cgtcgacttt ctgccgaaga tgaagatcga
     2221 actggtcgtc gccgacgcca ccgtcgatca ggcgatcgag gccatcatca aggccgcccg
     2281 caccggcaaa attggcgacg gcaagatttt cgtcatgccg gtcgatcagg tggtgcggat
     2341 tcgcacgggc gagacggacg aagccgcagt ctgacgctgg cagcattgct ccggcgaaag
     2401 gccggctgcg acgaagaacc ggggggcagc gatgccgagc ccggtttttt tgcgcctctt
     2461 tccctgtcac gccctgactc tctccgcatt gcgcgtgcag ccggccgttg gtcggcaaat
     2521 ccactccatt gatttagata ctccataagg gctattcatt gtcgtattaa tgcggcgcag
     2581 catgatccac gtttcataca cagggagaag ataagatgga ctcacgcagc ctgattcgcc
     2641 ccgtgctggc agccattttc gccggcatcg tttgtattcc gaccgcgttt gccgccgaga
     2701 ccgagtcggc cgtcgctcgc ggcggacgcc tttacgacaa gttctgggcc gagaacaagg
     2761 gcgccaagcc cgaggcagat cacgcggcct atccgcacaa gggcggcaag tacggcaagg
     2821 atgcctcctg gcgctgcaag gaatgccacg gctgggacta catgggcaag gacggcgcat
     2881 attcgtccgg cggccacgcc acgggcatca agggaatccg ggctgccgcg ggcaaggacg
     2941 tcgccgcgat cgtcgccgtg ctgaaggaca agactcacgg ctacaccgac cagcacctgt
     3001 cggcaaagga cttcaacgac gtcgcgctgt tcgtttccaa gggacaggtc gacatggaca
     3061 agtacattga tcgcgccggc aagaagtcca aaggtgacgc cggcaaaggc gaggtctact
     3121 tcaacaccat ctgcgccggc tgccatggca ccgacgggaa gaagatcaag gatgcggatc
     3181 cgctcgccgc ggcggcaggc aacccctggg aactgctgca caaggtcctg aacggccatc
     3241 cgggcgaatc gatgccggcg cttcgcgccc tcgacccgca gatcgccgcc gatctggtcg
     3301 cccacatgca gacgcttccg aagtaatgca tcgtcgggtc tgaagccgac ctcgagatac
     3361 gggcgcctgg tgcgcccgtg tcgttttctc caaccgtgat gacgctacgg gaagctggga
     3421 tgatcaaaag aacatgggcc tggctgcgac gaccggccgc caatctgtcg gccggcgtgc
     3481 tgctggtgct tggcggtctt ggtggcatcc tgttctgggg cggtttcaat accgccatgg
     3541 aatacaccaa ccgcctcgag ttttgcgttt cctgccacga gatgaatcag ctcgtgcatg
     3601 gcgaataccg ggaaagcgtc cattacagca acgcctcggg agtccgcgca atctgctcgg
     3661 attgccacgt gcctcgtccg tggggcgcca aggtgtggcg caagatcagg gccagcggcg
     3721 aggtatggca ttggctgcgc ggcaccatcg acacgccgga aaaattcgag gcgcagcgtt
     3781 tggtgatggc cgaacgggtc tggaaggaga tgaccgacaa caactcgcgc gagtgtcgga
     3841 actgccacga ctaccaggcg atggctttcc acaagcaatc gctccgcgca cgcgagaaga
     3901 tggaagaggc aaaggacaag aacaagccct gcatcgaatg ccataagggc gttgcgcacc
     3961 ggaagccgtt ggtcgagcga gacgactgac cgctggaaag gactgaggcg gtcgcggtgc
     4021 cgacgccacg ctggccgccg agcgtggtct ggcgagacat agaagcctcg ccgccggtca
     4081 gggacagaaa cgctcagccg ttcagtctgc ttgttccttc cggcactcgc ccatccgggc
     4141 taaagcccgg cgcctctgcg agcgcgcgcc gggccctccg ccatttcagc aacccgatac
     4201 gcacttcccg tccgcgtcaa actccatcgt cctgcccgac agcggcacat gcaccggcac
     4261 tttcgtcgcc ttgataaagg attcggtgcg cgtgccgggc ttgaccttgc cgccggcggt
     4321 ggctggctcg ttggcgtgcg agacgatgac cgacgccggc ctgaccagat cattgatgac
     4381 gtaggcggcc tcgttcgggc cggtggtgaa ggtatcgccg atgttgatca ccgccagttt
     4441 ggcaccgtaa tgcccgcgca ccaccttctc ctggtcgccg gtaatgcctg tgtcgccgga
     4501 gagatagacc gccaagccat tgctgaactt cagcacgtag ccggtggcct cgccgacgta
     4561 ggcgccgagg ccggcttcct tgagggtctt gccgagatcg ccgccgacca agtcgccgtc
     4621 gacggcattg ctgtggatcg ccgtcacggt ggcgatggtg acgccgccaa tggtttggct
     4681 ggcgccaaag cgtgccagca cagagttttt cgggtcgccg ccggcggctt tcagcttgtt
     4741 ggcgaagaag gtcggcattt cgctgccggt aacgatcttg gcgttcttgg ccagcgcgat
     4801 gtccacggcc aatgagttcg gcaacgccga aacggacata tccggcttgt cacaggcgcc
     4861 ggcgttgggg gccttggtgt gtttgtcgcc gacatggtcg ccgtgcatgt ggctgacgag
     4921 cacgacatcg atcttgccca gccgcgcatc ggcggcaccg gcgaccgtgc ggcccgggtc
     4981 gtagaggact cgtgtgccgt tcgggtcttc gaagaccagg gcgcgatcga ggatgcagaa
     5041 ctcgccgtcc tggccgccga gcggcgtgac cttgacgttt gcggcgaggg ccggttgggc
     5101 gagcagcgtg ctgagagcca gtacgccgag cagggatgcg actttcatgg tgacctccgg
     5161 tttggtgggc tgagcgggga gcatttctgg gccggctacc gatgatgctg aggggccggc
     5221 gatccggcct agcccagcgc gcgcagcgcg cggtcgtagt ccggctcctg cttgatttcg
     5281 gggaccagtt cggcgtcaag aacggtgtcg ttctcgtcga gcacgaccac ggcgcgcgct
     5341 gcgaggccct tgagcaggcc gctgtcgatc agcacgccat agtccttgag gaaattggct
     5401 ccacgcatcg tcgacaggtt gacgacattg gccaggcctt cggcaccaca gaagcggcct
     5461 tgcgcgaagg gcaggtcggc ggagatgcag aggacgacgg tgttcttcag cgccgaggcc
     5521 ttttcgttga acttgcgaac cgaggttgcg caggtcggcg tgtcgatgct gggaaagatg
     5581 ttgaggacct tgcgcttgcc ggcgaagtgc gccagcgtcg cgtcggacag gtccttggcg
     5641 accagcgaca gcggcggggc cttttgcccc ttcttcggga agtcgccggc gacggtaatt
     5701 gaattgccag cgtgggtgat cttgcttgcc atgttttgct cctcagattg aagtcggctg
     5761 agttgtaaag actccccggc aggcaattcg ttcagcgccg gcggcgatcc ggcctggcac
     5821 ggctcagcgt agcaaggctt gcgtgccggc taggacggcg agcgacatga gcgtcaccgg
     5881 gatgccgatt ctggcgtgct gcttccagtc gatctcgacg cccgtgcggc gggcgaggtc
     5941 ggcgacgatc aggttggcta tcgagccgac cagcagcagg ttgccggcaa aggtgctggc
     6001 cagcgccagc agcgttgccg cctcgctcga gtgcaggtgg ggcagcagca gcatgaccgc
     6061 cggcacgttc gacaccagat tcgagagccc gacgccgatg accagcagcg gccccggcgc
     6121 gtcgagcacg acgccgtgca cggccagcca gtgcacggca tcggcggcca ggccggttcg
     6181 ttcgaaagcg ccgttgacga cgaagaggcc ggcgaagagc accagcagcg gccagtcgac
     6241 gaagcccatc acctgcgccg aatgcagccg gcggctgagc agcaacacgc cggcggcgac
     6301 cagcgcgacc acctcgcgcg gccagtcggt gaacaggaag gcgagcatca ccagcgccgc
     6361 gacggcgagg cccttggccg actgccagcg gtcgaaggcc ggcgcgtcgt cggccgccag
     6421 atcgaccggg gtctggcgtt cggccagcag cccgggccga taggcgcggg cgagccacag
     6481 ccagagcgcg agcaggctca acagtaccgg cggcagcgcg gcgacgatgt agtcgacgaa
     6541 ggacagccgc gcaaccgcgc cgatcaacat gttctgcggg ttgccgatca gcgttgccgc
     6601 cgagccgatg ttggcggcgc aggcgaggcc gacgaggaat ggcaggggat tgaggctgcg
     6661 ctgcaggcag atgcgaacca gcaccggcgt taccgcgagg cagacgatgt cgttggaaaa
     6721 caccgccgag agcccggcga cgaccgcgat cagcgccgca agccacgctg ccggtgcgag
     6781 cggcagcgcc gcgacggcgc gggtgacggc ggtgtagaag ccgccgaggc gcatctgtgc
     6841 agacatcacc atgaaggcga agagcagcag cacggtcggc agatgtactg cctgcgcggc
     6901 cgattcgggc gacatcgcgc cgctgccgat gacggcgatg gcgccgagca gcgcgatgcc
     6961 ggcgcgatcg agcttcaggc gcggcaggcc gccgaggaac atgccgaggt agacgatggc
     7021 gaagatcgcg acgacggtcc agtcgaagct ggcagggtgg gcgggaagct ggggcatcgg
     7081 tggtgcgcgg gttgtgctgc cgccatcata tcggggcgat ggccgccggc agcatggcag
     7141 ggtgggggac aaaaaaatcc cgacgggcga acccgtcggg atggcgtgct gcggtgggcc
     7201 cggaggcccg gtatcgatgc ggcgcgctta gtgctgcgcg gcggcggcgg cgccgaagcc
     7261 ggtctgcgct cgcacgtact ggtcttcgaa cgcggcgact tcctgtgcgg cgcgtgccga
     7321 ggtctcgagc ttggagacga ggaaggcgag caggaaggcc accggcatcg cgaacagcgc
     7381 cggctgcgtg taggggaaga tcggcgagtc gaagccgaag acatcgaccc agaccgactt
     7441 cgacagcacg accagcagca cggccgaggt caggccgccg taaccgccgg cgagcgcgcc
     7501 acgcgtggtc aggcccttcc agtacatcga gagaatcagc accgggaagt tggcggctgc
     7561 tgcgaccccg aaggcgaggc cgaccatgaa ggcgacgttc tgcttctcga aggcgatgcc
     7621 gagcaggatg gcgacgaagc cgaggcagat cgtcgcgatc cgcgagacgc ggatctcgga
     7681 ggcctcgctg gccgtgccct tcttgatgac gcgggcgtag aggtcgtgcg agatcgccga
     7741 ggcaccggcg agcgccagac ccgagaccac cgcgagaatt gtcgcaaagg cgaccgcggc
     7801 gaggaagccg aggagcatgt cgccgccgac agccttggcc aggtgcatcg caaccatatt
     7861 gccgccgccg accagcttgc cgccaacgtc gccaccttca aagaactcgg ggttctggcc
     7921 gacgatcagg atcgctgcga ggcccattag caggatgacg ttgaagaaat aggcaacgaa
     7981 acccgaggcg tagagcactg acttgcgcgc ttccttagcg tcgctgacgg tgaagaagcg
     8041 catcaggatg tgcggcaggc cggcggtacc gaacatcaag ccgaggccga gcgaaatcgc
     8101 cgtgatcggg tccttgagca ggccgcccgg gtgcattagc ttgtccccga gcgagtggat
     8161 cgaggttgcc ttctccatca gtgtggtgaa cgagaagtcg aactggctca tcgccagcac
     8221 catcaccaac gtgccgccgg agagcagcat gcatgccttg atgatctgca cccaggtcgt
     8281 tgcgaccatc ccgccgaaag tgacatagac catcatcagg atgccgacgc cgaagatcgc
     8341 cacggtgtag tcgaggccga agagcagctt gatcagctgg ccggcgccga ccatctgcgc
     8401 gatcaggtag aagcagacca cggtcagcga cgagatcgcc gccatcgtcc gaaccttgcc
     8461 ctggtcgagg cggtaggcgg tgatgtcgga gaaggtgaac ttgccgaggt tgcgcaggcg
     8521 ctcggccatc aggaagagga tgatcggcca gccggcgaag aaggccagca tgtagaggta
     8581 gccgtcgtaa ccgcgcgaga agaccatcgc ggtgaggccg agaagcgtgg ccgccgacat
     8641 gtagtcaccg gcgatcgcca agccgttctg gaagcccgtg atgccgccac cggcggtgta
     8701 gaagtccgag gtcgacttgg tgcggctcga cgcccagtag gtgatgccca tggtcagcgc
     8761 gacaaagacg atgaacatcg tgatcgcgtg ccagttggtc ggctgcttct cgatcgcgcc
     8821 ttcgacggcg gggccggcga tggccaaagc gctcatggcg agcaggccga gagccgtgct
     8881 cagttgggtc aggcggcgca tcagcgattc tccttccagg cgtccttgac gatgtcgtca
     8941 gtcagcttgt cgaaatggga gttggcgcga cgcacgtaga gcgcggtcag cagccagaaa
     9001 aagacgaaca tgaacagctc ggcggcgacg ccgacggtcc acatcgagcc ctcggcaagc
     9061 ggcttgccaa tggtcgccgg gctaaaagcg acggtgagga cgaagccgta gaacatcgtc
     9121 aggacgattg cggccagggt ccaggcgaat cgccggcgct gcgtgacgag ttcctggaac
     9181 ttcggattgg cgcgcatgcg cccgtacatc gcactactca tggtcttttc tcccagattc
     9241 gtgaatgttc tgatatgcac cagacatgtg gattttatct tatataagac cggaatggtg
     9301 cgccgccgca aatatttacc ttggtttctc gaaacgcgtt acggaaaggg gaattccgcc
     9361 atatggcatt gcgatgaaag cggggttcgg atggccttcg aggggacgca cggcgggggt
     9421 gcggtgtcgg cagcgggcga cgccgccgca accggcgttc gggggcactt cgaggcgccg
     9481 aggggcgggg tggcacaatg ccgccttttt ccgccacccc gccgatgccc gctgattctc
     9541 ccgccaccga agcctgcctg agtgccgatc gcgcccggat cggcgggctg gcgcgtcggt
     9601 tggcgaaggc gcagggcgcc gagcgagaaa aggtcggcgt gcagctcgcg aaattgcggg
     9661 ccagttcggc cgccgccttc gctcgccgcg ccgaatcgct gccgcgtccc gagttttccg
     9721 acgagttgcc ggtcaacggc aggcgcgccg aaatcgccgc ggcgatccgc gatcatcaag
     9781 tggtcatcgt ctgcggcgaa accggctcgg gcaagaccac gcaattgccg aagatcctgc
     9841 tcgaactcgg catcggcgcc gccgggctga tcggccacac ccaaccgcgg cggctggccg
     9901 cgcgttcggt cgccgcgcgg ctggccgagg agctgaagac ctcggtcggg cggggggtcg
     9961 gcgtcaagat tcgcttccat gaccggaccg gccccgacgc ctgggtcaag gtgatgaccg
    10021 acggcatcct gctcgccgag acgcagggcg accaccagct gtcagcctac gaggcgatca
    10081 tcatcgacga ggcgcacgag cgcagcctca acatcgactt cctgctcggc tacctgaaga
    10141 ccctgctggc gcggcgatcc gacctgaagc tgatcgtgac ctcggcaacg atagacgccg
    10201 agcgtttcg
//
LOCUS       DATDAA010000045        11894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_73108,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000045 DATDAA010000000
VERSION     DATDAA010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11894
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73108"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            406..1428
                     /locus_tag="VIS73_01580"
     CDS             406..1428
                     /locus_tag="VIS73_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5761878.1"
                     /translation="MIKKNAFAIAAVATGLIFAGSAQAGATFDAVKKKGFVQCGVSTG
                     LPGFSAADDKGNWKGIDVDTCRAVAAAMFGDADKIKYSPLNAKERLTALQSGEIDVLS
                     RNTTWTFTRDTSLGLNFAGVNYYDGQGFMVSKKLKINTAKKLDGAAICIQAGTTTELN
                     VADYFRANKMKYKAITYDTSDETVKAFEAGRCDVLTSDASQLYALRIKLANPAGSIVL
                     PEIISKEPLGPVVRQGDDEWFNLVRWSLFAQINAEELGVTSANVDQMKSSSDPNVKRL
                     LGVEGVKGEMLGVKDDWAYQVIKQVGNYGEMFDRNVGAGSPLGIARGQNALWNQGGLQ
                     YAPPIR"
     gene            1527..2717
                     /locus_tag="VIS73_01585"
     CDS             1527..2717
                     /locus_tag="VIS73_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY5761879.1"
                     /translation="MSTEPTERDNPPQPSRLTDPKFRALVFQILATLTVAGIIYFIVT
                     NTLNNMEARGISTGFGFLDNTAGFAVLQSLIEYDETHTYGRTFLVGLLNTLLVSALGI
                     FFATILGFLMGIARLSHNWLINRIAMAYIEIFRNIPLLLQIFFWYFAIAINLPGPRDA
                     MSIGDIGFLSNRGLYLPAPIFEDGFGAVVVAFIAAVIAAVFMARWARRRQEATGQLFP
                     LYRSALALLVGVPAVVFLLAGTPLSWEIPELGGFNFQGGVSVIPELFALLLALSVYTA
                     AFIAETVRSGIQAVSHGQTEAAHALGLKHAWTLRLVIVPQAMRVIIPPLTSQYLNLMK
                     NSSLAAAIGYPDLVAVFAGTTLNQTGQAVEIIAITMSVYLTISLVISVFMNWYNAKMA
                     LRER"
     gene            2782..3831
                     /locus_tag="VIS73_01590"
     CDS             2782..3831
                     /locus_tag="VIS73_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY5761880.1"
                     /translation="MIGWLRKNLFATWPDVLLTLLAGYLLLMAIPPIIEWALIKADWV
                     GSDRTACDSGGACWVFVGARMQTFIYGFYPAEEAWRVNLFYGIALALLIPLFIDKFKY
                     KVWLGVMAILGLPLLSWVLVSGGMFGLEVVETAKWGGLSLTLMLAAVGCIAALPFGIL
                     LALGRQADNMPAVKTMCVVFIEFWRGVPLITVLFMSSVMLPLFLPEGVSFDKLLRALI
                     GITLFQSAYMAEVVRGGLQAIPKGQYEAADAMALTYWQKTGLIILPQALKIVIPGIVN
                     TFIALFKDTTLVIIIGLLEVLGMVQTALNDPNWLGYATEGYVFTALVFWIFCFGMSRY
                     SQNLEARLHTGHKKR"
     gene            3922..4650
                     /locus_tag="VIS73_01595"
     CDS             3922..4650
                     /locus_tag="VIS73_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY5761881.1"
                     /translation="MIEIREMHKWYGAFHVLKNINLQVRKGERIVVCGPSGSGKSTMI
                     RCINRLEEHQKGQIIVDGVELTSDLKQIERIRRDVGMVFQHFNLFPHLSVLDNCTLAP
                     IWVRKMPKKDAEEIAMQYLEKVKIPEQANKYPGQLSGGQQQRVAIARSLCMNPAIMLF
                     DEPTSALDPEMIKEVLDTMISLAESGMTMICVTHEMGFAKTVADRVIFMDKGEIVEEN
                     TPHEFFNNPQNERTQLFLSQILGH"
     gene            4653..6335
                     /locus_tag="VIS73_01600"
     CDS             4653..6335
                     /locus_tag="VIS73_01600"
                     /inference="COORDINATES: protein motif:HMM:NF006052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY5761882.1"
                     /translation="MAVPATRRSGGQILVDQLRLHGVTRAFGVPGESYLAVLDALFDS
                     SIDFVTCRHEGGAAMMAEATGKLTGRPGICFVTRGPGATNASAGVHIAFQDSTPLILF
                     IGQVARDQRDREAFQEIDYRRMFGQMSKWVAEVDDAARLPEYVSRAFHVATAGRPGPV
                     VLALPEDMLSDVFGVVDPAPYQPITVRPATADVDAIAARLARASRPLAIIGGGGWTAQ
                     AAADIARFADAWDLPVATSFRCQDYLDNTHRCYAGHVGIGIDAQLAERVRSADVLLVI
                     GARLGEMTTSGYKLLDIPKPRQALIHVFADASELGRVYQPEIGLVASPPAITAALAET
                     PPPFSVVPWAGAADAAHAGWREFNRPRPCAGDLQMAEIVAALRELTPPETVVANGAGN
                     YAIWVHRFHRWQHYRSQLAPTSGSMGYGVPAAIAAALADRQRPVICFAGDGCFMMSAA
                     ELATAVQLKAPVVFIVVNNGMYGTIRMHQERRYPGRVSGTALHNPDFAALARAFGMPG
                     QRIDRTAAFAPAFAAALAADGPTLLELAINPEALTPVETLSEIRAAAQANAP"
     gene            6403..7488
                     /locus_tag="VIS73_01605"
     CDS             6403..7488
                     /locus_tag="VIS73_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HEY5761883.1"
                     /translation="MNPASSTTNSKGEAQARADDIAVFRRELGRLEAEGVLALDAVQR
                     TAVSAHHGELLARLARQFDIDRDTPARQLSLGMRVASFLGALALAASVYFLFYQFWGN
                     FGEATQVTILIGAALGSFLGTLALQQRDAAGYFTKLAALVAFACFVLNIAMLGQIFNL
                     TPSDKALLPWAAYAFLLAYRCDLRLLLAVGILCASAWIAARVGTWGGLYWLSVGERPE
                     NFFPAAIVFFLLPAFIDHARFTGFAPVYRVFGLLLLLVPVLVLGLWGESSYLPGSASV
                     IEGFYQLAGFALAAGAIWLGARRHWNEVVNTGISFFVIFLYTKFFDWWWEAMPKYLFF
                     LILGLTAIGILVALRRLRARHAGGDPR"
     gene            7485..8396
                     /locus_tag="VIS73_01610"
     CDS             7485..8396
                     /locus_tag="VIS73_01610"
                     /inference="COORDINATES: protein motif:HMM:NF027432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4824 family protein"
                     /protein_id="HEY5761884.1"
                     /translation="MISLSQRQALIAGLALIVFTNAVTLGLVAWNRSGEPEAVLRLAE
                     RELERPWDWRGRHRESSGLTLTLRWRVAPIQTTDAESTIIDDEVEAWAYGGTAHWLDD
                     AKLTELGIAPPSDERRYRSRSHDALLVLEHDGDAYRRSLERARQHLARETALRDANSG
                     QREFADRVERANKRVVEQSEEASRLFVVDAGRDAAALRAKYPDRQRYAIVAGHIRPGW
                     HGPRQKRQRVAYIERIDAAAINVPHALRDAIPPDAGRHRGRPERDASNRFVAEIAFGR
                     RLEPWLLSVTGTPEEAPTAAENSGAAR"
     gene            8393..9247
                     /locus_tag="VIS73_01615"
     CDS             8393..9247
                     /locus_tag="VIS73_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5761885.1"
                     /translation="MNLRIAALTLLTMIAFAGNSLLCRIALRETGIDAASFTTIRLIS
                     GALVLWLIAAWLRRGRSGDGNWVSAAALFAYAAAFSFAYLDLSAATGALILFGAVQAT
                     MIGRGLAGGERFAPLQVVGLVLALAGLVGLLLPGLSAPPLGGSLLMLAAGIAWGVYSL
                     RGRGTGDPTRVTAGNFLRAVPMAAVLSIATLSGANLDLPGVGYAIASGALASGVGYAI
                     WYSVLPALKATHAATVQLSVPVIAAIGGVLLLTETVSLRLVLASIAILGGIALVILEK
                     RPVPAAAR"
     gene            complement(9260..9922)
                     /locus_tag="VIS73_01620"
     CDS             complement(9260..9922)
                     /locus_tag="VIS73_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY5761886.1"
                     /translation="MAESPIVILRKYTRALSTALGMRDDSTLRHSDRVQGLAQQLGTR
                     CGLDEYSLGILEVAASFHDIGKIGVRDSILMKPAKLDEDEWVEMRQHCDMGARIMDAT
                     GLEGATEAARAIRHHHEHYDGSGYPDGLAGEAIPLLSRIISVVDGYDAMAVTRAYHRG
                     RTHDAVIDVLQRETGGKYDPRLMAMFCELIETSEYRVRDSAVPHAATSGAEVMSEALR
                     AD"
     gene            complement(10055..>11894)
                     /locus_tag="VIS73_01625"
     CDS             complement(10055..>11894)
                     /locus_tag="VIS73_01625"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY5761887.1"
                     /translation="SAGLVLYEMLTGKQAVNGQDVYQVMHRLANEDIVLPLEARSEID
                     ERLVDILYKALARNPESRFSSVADFRQSLAEYLAGGGAAGPGSEQSTLDFLLRRMRHK
                     SDFPALSESVSAINKIAHSERDSVAKLSNTILRDVALTNKILRLVNSAFFRQAGAGNI
                     STVSRAVMVLGFDAVRNLAITVLLFEHLQKSPARNQLMEEFLKANFSALLGRDLAKKS
                     GGHDGEQVFICSLFHSLGRMLSQYYFPEETDSVRKLMQQKEISEAVASAQVLGISFDE
                     LGIGIARSWGFPPLIVNSMARLPDTPLRKARTPEESLHHVAGYANALCAVIAGTPVEE
                     RAKAMRRLSAQFGESIGLPDKDVEALVSKGIDDVAQFASVVHVNLKAMIVGKQIRAWA
                     GKPLVGLEAEEVDEETLAGTLQGTLLGARPSGGAGSGTSVGDDDNGLMPVDAQSVLAA
                     GIQDLSNTLVEDFKLNDVLRIVVETMYRALAFQRVIFCVRDARSNSMVGRFGFGPDAS
                     EMLRHVRFSLQGDPDVFLAAVTRGVDILISDTNDEHIKGRIPGWYRKAFTAQTFVLLP
                     LTIKSRPVALIYADREHAGEIVIPEKELNLLRTLRNQALLAIKQAG"
BASE COUNT         2041 a         4066 c         3670 g         2117 t
ORIGIN      
        1 ggcatcctcg aaaccccgca aggcagcaag gcgccaaaga cgatgcaagg caaactctgc
       61 ggcgaatgcg gcaaccacac ggtgatccgc aaggatggct gcgacttctg cagtgcctgc
      121 ggggcggtgg gcgcctgcgg ctagcaaacc actcgaccaa gttccagaaa gaccccgggg
      181 cgtttggccc ggggtttttt gttgttcgtt gccgctactt tcgcacgata aacagcacgg
      241 agcgttgcca gaaaattgct gcactgcgcg atggacacgg tttgcaaatc ggcctgacgg
      301 ttgctttaat acggtcgttc gaacgaaaca tcatcaccga tcggccccca ccagggaact
      361 ccaccgatca ccacacccaa cgtatccctg taggagacaa cccgaatgat caaaaagaac
      421 gcgttcgcaa ttgccgccgt tgccaccggc ctgattttcg ccgggtcggc ccaggccggc
      481 gcaacctttg atgccgtcaa gaagaagggc ttcgtccagt gcggggtcag cactggcctg
      541 ccgggattct cggccgccga cgacaagggc aactggaagg gcatcgacgt cgacacctgc
      601 cgcgccgtcg ccgcggcgat gttcggcgac gccgacaaga tcaagtacag cccgctcaac
      661 gccaaggagc gcctcaccgc gctgcagtcg ggcgaaatcg acgtgctgtc gcgcaatacc
      721 acctggacct tcacgcgtga caccagcctc ggcctcaact tcgccggcgt gaactactac
      781 gacggccagg gcttcatggt cagcaagaag ctgaagatca atactgccaa gaagctcgac
      841 ggcgccgcca tctgcatcca ggccggaacg acgaccgagc tcaacgtcgc cgactacttc
      901 cgcgccaaca agatgaagta caaggcgatc acctacgata cctcggacga gaccgtgaaa
      961 gccttcgaag cgggccgctg cgacgtgctg acctcggatg cctcgcagct ctatgcgctg
     1021 cgcatcaagc tggccaatcc cgccggatcc atcgtcctgc ccgagatcat ctccaaggag
     1081 ccgctcggcc cggtcgtccg ccaaggggac gacgaatggt tcaatctggt ccgctggagc
     1141 ctattcgcgc agatcaacgc cgaagagctg ggcgtaacct cggccaacgt agaccagatg
     1201 aagtcgagtt cagaccccaa cgtcaagcga ctgctcggcg tcgagggcgt caagggcgag
     1261 atgctcggtg tcaaggatga ctgggcctac caagtcatca agcaggtcgg caactacggc
     1321 gagatgttcg atcgcaacgt cggcgcaggc agcccgctcg gcatcgcccg cggccagaac
     1381 gccctgtgga accagggcgg cctgcagtac gccccgccga ttcgctgagg cctgacgcat
     1441 cagcccgatg cgcggcggga gtcaaggctc tcgccgcgtg ttgtttcgac ctgttcctga
     1501 ttcaccttcc atcaccgagg tgaccgatga gcaccgagcc aaccgaacgc gataacccgc
     1561 cgcagccctc gcgcctgacg gacccgaaat tccgcgcgct ggtcttccag attctcgcga
     1621 ctctgacggt cgctggcatc atatatttca tcgtcaccaa caccctgaac aacatggagg
     1681 cccgcggcat ttcgacgggc ttcggcttcc tcgacaacac ggccggcttc gccgtattgc
     1741 agtcgctgat cgaatacgac gagacgcaca cctacggccg gaccttcctc gtcggcctgc
     1801 tcaatacgct gctggtttcc gcgctgggca tattcttcgc gacgatcctg ggttttctca
     1861 tgggcatcgc gcggctctcg cacaactggc tgatcaaccg gatcgcgatg gcctacatcg
     1921 agatcttccg caacatcccg ctgctgctgc agatcttctt ctggtacttc gcgatcgcca
     1981 tcaacctgcc gggcccgcgc gacgcgatgt cgatcggcga catcggcttc ctcagcaacc
     2041 gcggcctcta cctgccggcg ccgatcttcg aggacggctt cggcgccgtc gtcgtcgcct
     2101 ttatcgcggc cgtaatcgcc gccgtcttca tggcgcgctg ggcgcgccgc cgacaggagg
     2161 cgaccgggca gttgttcccg ctctaccgca gcgcgctggc gttgttggtc ggcgtaccgg
     2221 ccgtcgtttt cctgctcgcc ggcacgccgc tgtcgtggga aattcccgaa ctcggcggct
     2281 tcaatttcca gggcggcgta tcggtgatcc ccgagttgtt tgcgctgctg ctggcgctat
     2341 cggtctacac cgccgccttt attgccgaga ccgtgcgcag tggcatccag gccgtttcgc
     2401 atgggcagac cgaggcggcg cacgcgctgg gcctcaagca cgcctggacg ctgcggctgg
     2461 taatcgtgcc gcaggcgatg cgcgtcatca ttccgccgct gaccagccag tacctcaacc
     2521 tgatgaagaa ttcctcgctc gcggcggcga tcggctaccc cgacctcgtt gcggtattcg
     2581 ccggcacgac gctcaaccag accggccagg cggttgagat catcgccatc acgatgagcg
     2641 tgtacctgac gatcagcctg gtgatctcgg ttttcatgaa ctggtacaac gcgaagatgg
     2701 cgttgcggga gcgctgagat ggccgacgcc aaacagtttg ttccgtcccc cagcctaccg
     2761 ccacccgtaa gcgaagtcgg agtgatcggt tggttgcgca agaacctctt cgcgacctgg
     2821 cccgacgtcc tgctgaccct gctcgccggc tacctgctgc tgatggcaat tccgccgatc
     2881 atcgaatggg cgttgatcaa ggccgactgg gttggctccg accggacagc ctgcgactcg
     2941 ggaggcgcct gctgggtgtt cgtcggcgcc cgcatgcaga ccttcatcta cggcttttac
     3001 ccggctgagg aagcttggcg cgtcaacctc ttctacggca ttgcgctggc gctgctgatc
     3061 ccgctgttca tcgacaagtt caagtacaag gtctggctag gcgtaatggc gatcctcggc
     3121 ctgccgctcc tgtcgtgggt tctcgtttcc ggcggcatgt tcggactcga agtcgtcgag
     3181 accgccaaat ggggcggcct cagcctgacg ttgatgctcg ccgcggtcgg ctgcatcgcc
     3241 gcgctgccat tcgggatcct gctggcgctg ggccggcagg ccgacaacat gccggcggtg
     3301 aaaaccatgt gcgtggtctt catcgagttc tggcgcggcg taccgctgat caccgtgctc
     3361 ttcatgtctt cggtgatgct gccgctgttc cttcctgaag gcgtcagctt cgacaaatta
     3421 ctgcgcgcgc tgattggcat cacgctgttc cagtcggcgt acatggccga ggttgttcgc
     3481 ggcggcctgc aggcgattcc caagggccag tacgaagccg ccgatgcgat ggcactaacc
     3541 tactggcaga agacgggcct gatcatcctg ccgcaggcgc tgaagatcgt cattcccggc
     3601 atcgtcaata ccttcatcgc gcttttcaag gacacgacgc tggtcatcat catcggcctg
     3661 ctcgaagtgc tgggcatggt ccagactgcg ctcaacgacc cgaactggct gggctacgca
     3721 accgagggct acgtcttcac ggcgctggtc ttctggatat tctgtttcgg catgtcgcgc
     3781 tacagccaga acctagaagc gcgcctgcat accggccaca agaaacgcta accggctgcc
     3841 cgtccagccc gcgtggagtc aatacccatg aaccagaccg ctgaaaaagc ctcgaccgac
     3901 cgcagcctct ccgatcacgt catgatcgaa atccgcgaga tgcacaagtg gtacggcgcc
     3961 ttccatgtac tgaagaacat caacctgcag gtcaggaaag gcgagcgcat agtcgtctgc
     4021 ggcccctcgg gatcgggcaa gtcgacgatg atccgctgca tcaaccggct tgaggagcac
     4081 cagaagggcc agatcatcgt tgatggcgtc gagctgacgt cggacctcaa gcagatcgag
     4141 cgcattcggc gcgatgtcgg catggtcttc cagcacttca acctgttccc gcacctgtcc
     4201 gtcctcgaca actgcacgct ggcgccgatc tgggtgcgca agatgcccaa gaaggacgcc
     4261 gaggaaatcg cgatgcagta ccttgagaag gtcaagattc ccgagcaggc gaataaatat
     4321 cccggccagc tctccggcgg ccagcagcag cgcgtggcga tcgcccgcag cctgtgcatg
     4381 aacccggcga tcatgctctt cgacgaacca acctcggcgc tcgacccgga gatgatcaaa
     4441 gaagtgctcg acacgatgat cagcctagcg gagtcaggca tgacgatgat ctgcgtgacg
     4501 cacgagatgg gcttcgccaa gaccgtcgcc gaccgcgtga tctttatgga caagggcgaa
     4561 atcgtcgaag aaaacacgcc gcacgaattc ttcaacaacc cgcagaacga acggacccag
     4621 ctcttcctga gccagattct cggccactga gcgtggcggt tccggcaacg cgtcgcagcg
     4681 gcggccagat cctggtcgac cagctgcgac tgcacggcgt cacgcgcgcc ttcggcgttc
     4741 ccggcgaaag ctacctcgcg gtgctcgacg cgctgttcga ttcgagcatc gacttcgtca
     4801 cctgccgcca cgaaggcggc gcggcgatga tggccgaagc gaccggcaag ctcaccggca
     4861 gacccggcat ctgcttcgtc acccgcggcc ccggcgcgac caacgccagc gccggcgtgc
     4921 acatcgcctt ccaggactcg acgccgctga tcctcttcat cggccaggtg gcacgcgacc
     4981 agcgcgaccg cgaggcattc caggaaatcg actaccgccg catgttcggc cagatgtcca
     5041 agtgggtcgc agaggtagac gacgcggcgc gcctgcccga gtacgtgagc cgcgcgttcc
     5101 atgtcgccac cgccggacgt cccggcccgg tcgtgctcgc gctgcccgag gacatgctca
     5161 gcgacgtgtt cggggtcgtt gatccagccc cgtaccagcc gattacggtg cggcccgcga
     5221 ccgccgacgt cgacgcgatc gccgcacggc tggctcgcgc cagccgaccg ctggcgatca
     5281 tcggaggtgg cggctggacg gcgcaggctg ccgccgacat cgcgcggttc gccgacgcct
     5341 gggacctgcc ggtggcgacc tccttccgct gccaggacta cctcgacaac acccatcgat
     5401 gctatgccgg ccacgtcggc atcggcatcg atgcgcaact cgcggagcgg gtccgctcgg
     5461 ccgatgtgct gctggtcatc ggcgcgcggc tgggcgagat gactaccagc ggctacaagc
     5521 tgctcgacat cccgaagccg cggcaggcgc tgatccatgt ctttgctgac gccagcgaac
     5581 tcggtcgcgt ctaccagccc gagatcggcc tcgtcgccag cccgccggct atcaccgctg
     5641 ctctcgccga gacgccaccg ccgttttcgg tggtgccctg ggccggcgcg gccgatgccg
     5701 cacacgccgg ctggcgcgaa ttcaaccggc cacggccctg cgcaggcgac ctgcagatgg
     5761 ccgagatcgt cgccgcgctg cgcgagctga cgccgccgga gaccgtcgtc gccaacggcg
     5821 ccggcaacta cgcaatctgg gtccaccgct ttcaccgttg gcagcactac cgcagccagc
     5881 tcgcgccgac ctcgggttcg atgggctacg gcgtcccggc ggcgatcgcc gcggcgctag
     5941 ccgaccgcca gcggccggtc atctgtttcg ccggcgacgg ctgcttcatg atgagcgccg
     6001 ccgagctggc gaccgccgtg caactaaagg cccccgtcgt cttcatcgtc gtcaacaacg
     6061 gcatgtacgg caccatccgc atgcaccagg aacgccgcta tcccggccgc gtcagcggca
     6121 cagccctgca caatccggat ttcgccgcgc tggcccgcgc cttcggcatg cccggccagc
     6181 gcatcgatcg cacagcagct ttcgcgcccg ccttcgccgc cgcgctggct gccgacgggc
     6241 cgacactgct cgagctggcg ataaaccccg aggcgctgac gccggtggag acgctctcgg
     6301 aaatccgcgc cgcagcgcag gctaacgcac catagcgagc ttggcagggc gcggccagcg
     6361 gccgacaatt cgcccatcca gcgcgcaacg gcaatcccga ccatgaatcc ggcaagctca
     6421 acaacgaaca gcaagggcga ggcgcaggca cgcgccgacg acatcgcggt gttccgccga
     6481 gagctcggtc gcctcgaagc cgaaggcgtg ctggccctcg acgcggtgca gcgcacagcc
     6541 gtatccgcgc atcacggcga gctgctcgca cggctggctc ggcagttcga catcgatcgc
     6601 gatacgccgg cccgtcagct ttcgctgggc atgcgcgtcg cctccttcct tggtgcgcta
     6661 gcgctcgccg cgagcgtcta tttcctcttc tatcagttct ggggaaactt cggcgaagcg
     6721 actcaggtga cgatcctgat cggcgccgca ctcggcagct tcctcggcac actggcgctg
     6781 cagcaacgcg atgccgccgg ctacttcacc aagctcgcgg cgctggtcgc cttcgcctgc
     6841 ttcgtgctca acatcgccat gcttgggcag atcttcaacc tgacgccgag cgacaaggcg
     6901 ctgctgccct gggcggccta cgcgttcctg cttgcctacc gctgcgacct gcgcctgctc
     6961 ctcgccgtcg gcatcctctg cgcaagcgcc tggatcgccg cccgcgtcgg cacctggggc
     7021 gggctctact ggctctcggt cggcgaacgc cccgagaact tctttccggc ggcgatcgtg
     7081 tttttcctgc tgccggcctt catcgaccac gcacgcttca ccggctttgc cccggtctat
     7141 cgcgtcttcg gcctgctcct gctgctagtg ccggtactcg tgctcgggct gtggggcgag
     7201 tcgagctacc tgccgggttc ggcgagcgtc atcgagggct tctaccaact cgccggcttt
     7261 gcgctcgcgg ccggcgcgat ctggctcggc gcacgccgcc actggaatga ggtagtcaat
     7321 accggcatca gcttcttcgt cattttcctt tacaccaagt ttttcgactg gtggtgggag
     7381 gcgatgccga agtacctgtt cttcctgatc ctcggcctca cggcgatcgg catcctcgtc
     7441 gcgctgcgcc gcctgcgcgc gcgacacgcc ggcggagacc cgcgatgatc agcctgtcac
     7501 aacgccaggc cctgattgcc ggactggcgc tgatcgtctt caccaatgcg gtgacgctag
     7561 gactggtcgc ctggaatcgc agcggcgaac ccgaggccgt gctcaggctc gccgaacgtg
     7621 aactcgaacg cccctgggac tggcgcggcc gccaccgcga aagcagcggc ctaacgctga
     7681 ccctgcgctg gcgcgtcgcc cctatccaaa ccaccgacgc cgagagcacc atcatcgacg
     7741 acgaggtcga ggcctgggcc tacggcggca ccgcccactg gctcgacgac gccaagctca
     7801 ccgaactcgg catcgcgccg ccgtccgacg aacggcgcta tcgcagccgc agccacgacg
     7861 cgctgctcgt gctcgaacac gatggcgatg cctaccgccg ttcgctcgaa cgcgcacgcc
     7921 agcatctcgc tagggaaacg gcgctgcggg acgccaattc cggacagagg gaattcgccg
     7981 atcgtgtcga gcgcgcaaac aaacgcgtcg tcgagcaaag cgaggaggcg agccgcctgt
     8041 tcgtcgtcga tgccggacgc gatgcggcgg cgctgcgcgc gaagtacccc gaccgccagc
     8101 gctacgccat tgtcgccggg cacatccggc ccggttggca cggcccccgg cagaagcgcc
     8161 agcgcgtcgc gtacatcgaa cgcatcgacg cggcagccat caacgtgccg cacgcgctgc
     8221 gcgacgccat accacccgat gccggacgac atcgcggccg acccgagcgc gatgccagta
     8281 accgcttcgt cgccgagatc gccttcggcc gccgcctcga accgtggctg ctcagcgtga
     8341 ccggcacgcc ggaagaggca ccgactgcgg cagagaattc aggagcggca cgatgaatct
     8401 tcgtatcgcg gcgctgacgc tgctgaccat gatcgccttc gccggcaact cgctgctttg
     8461 ccgcatcgcg ctgcgcgaga ccggcatcga cgcggccagc ttcaccacca tacgactgat
     8521 ttccggcgcc cttgtactct ggttgatcgc cgcctggctg cgccgcggca ggagcggcga
     8581 cggcaactgg gtttcggcgg cggccctttt cgcctacgct gccgcgttct ccttcgccta
     8641 cctcgacctc tccgccgcga ccggcgctct gatcctcttc ggcgccgtgc aggcgacgat
     8701 gatcggccgc ggactggcag gcggcgaacg ctttgcgcca ctgcaagtgg tcggcctcgt
     8761 gctcgcgctg gccggcctgg tcggcctgct gctgccggga ctcagcgcgc cgccgctggg
     8821 cggctcattg ctgatgctag ccgccggcat cgcttggggc gtttactcgc tgcgcggacg
     8881 cggtaccggc gatccgacac gggtgactgc cggcaacttc ctgcgcgcag taccgatggc
     8941 cgccgttctc agcatcgcca cgctgtccgg cgccaacctc gacctgcctg gcgtcggcta
     9001 cgcgattgcg tcgggggcgc tggcctcggg cgtcggctac gcgatctggt actcggtact
     9061 gccagccttg aaggcgacgc acgcggcgac ggttcagctc tcggttccgg tgatcgcggc
     9121 aatcggcggc gtgctgttac tcacggaaac cgtcagccta cggctggtac tggcgtcgat
     9181 cgccattctc ggcggcattg cactggtgat tctcgagaag cgaccggtgc ccgccgccgc
     9241 gcgctaaccg gcccgctcgt cagtcggcgc gcagcgcctc gctcatcact tcggcaccag
     9301 atgtcgcggc gtgcggcacc gccgaatcgc gtacgcggta ctcgctggtc tcgatcagct
     9361 cgcagaacat cgccatcaag cgcggatcgt acttgccgcc ggtttcgcgc tgcaggacat
     9421 ctatcaccgc gtcgtgcgtg cgaccgcggt ggtaggcgcg agtgacggcc atcgcgtcgt
     9481 agccgtcgac gacgctgatg attcgcgaga gcagcggtat tgcctcgccg gccaagccat
     9541 cgggatagcc gctgccgtcg tagtgctcgt ggtggtggcg tatcgcgcgt gcggcctcgg
     9601 tggcgccttc gagtccggtc gcatccatga tccgcgcgcc catgtcgcag tgctggcgca
     9661 tctcgaccca ttcatcctcg tcgagcttcg ccggcttcat caggatcgag tcgcgcacgc
     9721 cgatcttgcc gatatcgtga aacgaggcgg cgacttcgag aatgccaagg ctgtattcgt
     9781 ctagcccgca gcgcgtgcca agttgctgtg ccaagccctg tactcggtcc gaatggcgca
     9841 atgtcgagtc atcacgcatg cccagcgcgg tcgataaggc tctggtgtac tttcgcaata
     9901 tgacaatcgg cgattcggcc atcgtgtgca cgctccctgg cgttgtcgac ggacagcgcg
     9961 ccggcgcggg actgctccgg gcggcatcga cagtgcttag aagcggctcg gcccgatagg
    10021 tttccgattc gctccaatca tggggaagcg tggttcagcc cgcctgcttg atcgccagca
    10081 gcgcctggtt gcgcagcgtg cgcaacaggt tgagctcttt ctcagggatg acgatttcgc
    10141 cggcatgctc gcggtcagcg tagatcagcg ccaccgggcg actcttgatc gtcagcggca
    10201 gcaggacgaa ggtctgcgcg gtaaaggcct tacggtacca accggggata cggcccttga
    10261 tgtgctcgtc gttggtgtcg ctgatcagga tgtctacgcc acgcgtcacc gcggcaagga
    10321 agacgtcggg gtcaccctgc agcgagaagc gcacgtgccg cagcatctcg ctggcatcag
    10381 ggccgaagcc gaagcggccg accatcgaat tgctgcgtgc gtcgcgcacg cagaagatga
    10441 cgcgctggaa agccagcgcc cggtacatcg tctcgaccac gattcgcagc acgtcgttga
    10501 gcttgaagtc ctcgaccagg gtattgctga ggtcctgtat gcccgccgct agtactgact
    10561 gcgcgtcgac cggcatcagg ccgttgtcgt cgtcgccgac actggtgccg ctgcccgcgc
    10621 caccggaagg ccgtgcgccg agcagggttc cctgcaaagt cccggctagc gtttcctcgt
    10681 cgacttcctc tgcttccaga ccgaccagcg gcttgccggc ccaggcacgg atctgcttgc
    10741 cgacgatcat cgccttgagg ttgacgtgca ccaccgaggc aaactgcgcg acatcgtcga
    10801 tgcccttgct gaccagcgcc tcgacgtcct tatcgggcag gccgatgctc tcgccgaatt
    10861 gcgccgacag gcggcgcatc gccttcgccc gctcctcaac cggcgtgccg gcgatcaccg
    10921 cgcacagcgc gttggcgtag ccggcgacgt ggtgcaggct ttcctcaggc gttctcgcct
    10981 tgcgcagcgg cgtatcgggc aggcgcgcca tgctgttgac gatcagcggc ggaaaccccc
    11041 acgagcgagc gatgccgatg cccagttcgt cgaaggagat gccgagaacc tgcgccgacg
    11101 cgaccgcctc ggatatctcc ttctgctgca tcaatttgcg caccgaatcg gtttcttcgg
    11161 ggaagtagta ctggctcagc atgcgaccga gcgaatgaaa cagcgagcag atgaagacct
    11221 gctcgccgtc gtgcccaccc gatttcttcg ctagatcgcg cccgagcagc gcgctgaagt
    11281 tggctttgag aaactcctcc atcagctggt tgcgcgccgg cgatttctgc aggtgctcga
    11341 agagcagcac ggtaatcgcc agattgcgca cggcgtcgaa gcccagcacc atcaccgccc
    11401 gcgacaccgt gctgatgttg ccagcaccgg cctgacggaa gaaggctgag ttgaccaggc
    11461 gcaggatctt gttggtcagc gcgacgtcac gcaggatcgt gttagaaagc ttggcaacgc
    11521 tgtcgcgctc cgaatgggcg atcttgttga tcgcactgac cgactccgag agcgccggga
    11581 aatcgctctt gtgccgcatc cggcgcaaga ggaagtcgag cgtgctctgc tccgatccgg
    11641 ggccggctgc gccgccgccg gcaaggtact cggcgagcga ttggcggaaa tcggcaacgc
    11701 tggaaaaacg cgactccgga tttctcgcca gcgccttgta gaggatgtcg accagacgct
    11761 cgtcgatctc gctgcgcgcc tcgagcggca gtacgatgtc ctcgttggcc agccggtgca
    11821 tgacctggta gacgtcctgg ccgtttaccg cctgcttgcc ggtgagcatt tcgtagagca
    11881 cgagcccggc cgag
//
LOCUS       DATDAA010000046         2223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_73255,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000046 DATDAA010000000
VERSION     DATDAA010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73255"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1692)
                     /locus_tag="VIS73_01630"
     CDS             complement(<1..1692)
                     /locus_tag="VIS73_01630"
                     /inference="COORDINATES: protein motif:HMM:NF009589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY5761888.1"
                     /translation="MAQTQAAIPLDNYELEDRYRRESGRVFLTGTQALVRIPLMQRTI
                     DHAAGLNTAGFISGYRGSPLGAYDMELWRASRYLEESNIRFLPAVNEELAATAILGTQ
                     QVEGDPQADVDGVFGIWYGKGPGLDRAGDGLKHGNAYGSSPRGGVLVVAGDDHGCVSS
                     SMPHQSDVAFLTWLMPNLNPASVAEYLDFGLYGIALSRFSGMWVGFKAISETVESAMS
                     VELKPYPQFVLPEDFVVPPGGLHIRWPDYPGPQIEERLIAKKAAPLAFAEANPNDRRI
                     VGTPDARSGIVTTGKGHLDLMEALRLLGIDRREAHRLGIDVYKVGMVWPLAHGSALDF
                     VRGKQEVLVIEEKRGLIESQFKEYFYDYPGRKPERMVGKEDELGNKLVPWVGELTPLK
                     LAGILASRLDKALPGLRLSDRANSLVESSAPLIDIAGADRTPYFCSGCPHNTSTRVPD
                     GSHALAGIGCHFMANWMDRDTDGLIQMGGEGVNWITRSMFNGGRHVFQNLGDGTFYHS
                     GSLAIRQAIAAGTNITFKILVNDAVAMTGGQPVDGPITVERIAHSVRAEGVDRVAL"
     gene            1855..>2223
                     /locus_tag="VIS73_01635"
     CDS             1855..>2223
                     /locus_tag="VIS73_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY5761889.1"
                     /translation="MDKKDRLILKELQHDSRLTMQQLAERTGMSTSSCWRRVRQLEDE
                     GVIDRYAAILNPKKAGFALSSMTLVSLARHEQKHVDNFIQEVLRHPEVLECFATSGEA
                     DFHLRVVVEDIDAYNRFLDDF"
BASE COUNT          408 a          690 c          714 g          411 t
ORIGIN      
        1 gagcgcaacg cgatcgacgc cctcggcccg gaccgaatgc gcgatacgtt ccacggtgat
       61 cggtccgtcg accggctgtc cgccggtcat cgccaccgcg tcgttgacca ggatcttgaa
      121 ggttatgttg gtgccggcgg cgatggcctg gcggatcgcc agcgagcccg agtggtagaa
      181 ggtgccgtcg ccgaggttct gaaacacgtg cctgccgccg ttgaacatgg agcgggtgat
      241 ccagttgacg ccctcgccac ccatctggat caggccgtcg gtgtcacggt ccatccagtt
      301 ggccatgaaa tggcagccta tgccggccag cgcatgcgaa ccgtcgggca ccctggtcga
      361 ggtattgtgc gggcaacccg agcaaaaata aggcgtcctg tcggcgcccg cgatgtcgat
      421 gagcggcgcg ctcgattcga ccaggctgtt cgcgcgatcg cttagccgca ggcccgggag
      481 tgccttgtcg agcctcgagg ccaaaatgcc ggcgagtttg agcggcgtga gctcgccgac
      541 ccacggcacg agcttgttgc ccagttcgtc ctccttgccg accatgcgct cgggcttgcg
      601 tcccgggtag tcgtagaaat attccttgaa ctggctctcg ataaggcccc gcttttcctc
      661 gataaccagg acttcctgct tgccgcgcac gaaatcgagc gcagagccgt gcgcgagcgg
      721 ccagaccatg ccgaccttgt aaacgtcgat ccccagccgg tgcgcttccc gccggtcgat
      781 gccgagcagg cgcagcgctt ccatgaggtc gaggtggccc ttgccggtcg tgacgatgcc
      841 ggagcgtgca tccggagtgc cgacgatgcg gcggtcgttc gggttcgcct cggcgaatgc
      901 cagtggtgcc gccttcttgg cgatcaggcg ttcctcgatc tgcggcccgg ggtagtcggg
      961 ccagcgtata tgcagcccgc cgggcgggac gacgaaatcc tcgggcagga cgaactgcgg
     1021 gtagggtttc agttccaccg acatcgccga ctcgaccgtc tccgatatcg ccttgaagcc
     1081 cacccacatg ccggagaagc gcgacagcgc gatgccgtaa agtccgaagt ccaggtactc
     1141 ggcgacgctg gccgggttca ggttcggcat gagccaggtc aggaaggcga cgtcggactg
     1201 gtgcggcatc gacgaggaga cgcagccgtg atcgtcgccg gccacgacca gcacgccgcc
     1261 gcggggcgag ctgccgtagg cgttgccgtg cttcaggccg tccccggccc ggtccagccc
     1321 ggggcccttg ccgtaccaga tgccgaatac gccgtcgacg tcggcttgcg ggtcaccctc
     1381 gacctgctgc gtgccgagaa tggcggtggc cgcgagttcc tcgttgacgg cgggcaggaa
     1441 gcggatgttg ctctcctcga ggtagcgcga ggctcgccac aattccatat cgtaggcgcc
     1501 aaggggcgag ccacggtagc cgctgatgaa tcccgccgta ttcagaccgg ccgcgtggtc
     1561 gatggtgcgt tgcatcagcg ggatgcgcac gagcgcctgg gtgccggtca ggaacacgcg
     1621 gccggattcg cgccggtagc gatcctccag ttcgtagttg tcgagcggaa ttgccgcctg
     1681 tgtctgggcc attgtgaggt ttcctgggga gtgccgggga ccattgtcgc agaccggccg
     1741 cggatgtcga tgccaaaata gcccgaaaaa agcaattgaa aagataaata cttatagata
     1801 atcaaaaatt aatggtaata tttttcaatt atttcaagta ctcggaaaat ttgcttggat
     1861 aagaaagacc gcctgattct aaaggaactg cagcacgaca gccgcctgac catgcagcag
     1921 ctcgctgagc gcaccggcat gtcaacgtcg tcatgctggc gccgcgttcg ccagctcgaa
     1981 gacgaaggcg tcatcgaccg ctacgcggca atcctcaatc cgaaaaaagc cggcttcgcg
     2041 ctctcgtcga tgaccctggt ctcgctcgcc cggcacgagc agaaacacgt cgacaatttc
     2101 atccaggaag tgctgcgcca cccggaggtc ctcgagtgtt tcgcgaccag cggggaggcc
     2161 gattttcacc tgcgggttgt cgtggaagac atcgacgcct acaaccgctt cctcgacgat
     2221 ttc
//
LOCUS       DATDAA010000047        10403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_457783,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000047 DATDAA010000000
VERSION     DATDAA010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10403
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457783"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VIS73_01640"
     CDS             complement(<1..419)
                     /locus_tag="VIS73_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761890.1"
                     /translation="MDLIRFQARLGGCLRGIVALLLLCSATAQAQVFDRIDVVAGERE
                     AEIVVRFSHRIQYLRHGPGGEVREARVFLRLLTSGVTEDQLMQDTVRSPVVAGVPVVK
                     AVFPELRNGMLLTFSQPTRYTVKPGADGRSIVLTVPRL"
     gene            complement(419..4975)
                     /locus_tag="VIS73_01645"
     CDS             complement(419..4975)
                     /locus_tag="VIS73_01645"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY5761891.1"
                     /translation="MRTLPRIAGALARSLVILMALAAVPAAAQNISGSGSFNHATVGY
                     TLDGAHVRERCESCHRQGLFRGTPRDCAGCHRSGGRAPGKTPSHVPTTTACDACHLTS
                     NWSISTFRHQPAQGVSVGSCAICHNGSSAHGKPANHIVTNAACDSCHRSTAWTPAGYT
                     HALATPGSCQSCHNGIRATGRSIRHIPTSTSCEQCHAIGSAFAPTTTSTATLHSAMSG
                     PVAAGNCAMCHNGNYTAQNAQAKPASHIPTSEQCDSCHSSTTTWATASFDHGSLSPPA
                     TGICSSCHGTSALGKSSSHVPTNLQCDLCHVGFTAFKPAQMNHFSSAAQCVGCHGGTY
                     VSAGARTKPVSHIPTTGQCDACHASTTGWTPAAMNHAGTAGKCIGCHSGGFLAQNAQV
                     KHATHVATTAQCDTCHKSTTTWATRIFDHSSATPAVAGRCSSCHNSVNALGKPTTHVP
                     TSAQCDSCHSGFAAFRPARMNHAGTAGQCASCHNGNYAFANADGRPANHIPTSASCDS
                     CHTGGFTAWRPATMNHAGTTGLCSACHSGTYLAMNAQAKPPTHVATTAQCDSCHKSTT
                     TWASATFNHATAVPAVAGRCSSCHGVNALGKPTNHVPTTVQCDSCHGNFTAFAPATMN
                     HAATTGQCASCHNGTYLFAGALAKPGNHVPTTAQCDTCHRSTSSWTGAAFTHGPSAVG
                     TCSTCHNGSTSAPAKSGAHIPTGLQCDNCHTNYTAFVPATMNHAGLAGQCQSCHGGSH
                     TAVGALAKSTSHVPTSVQCDVCHVNGFLSFAPGRMNHAATAAPVAAGNCSTCHGGAYV
                     SQNALAKPATHVATTAQCDSCHQSTTSWATATFNHATASPPVAGRCASCHGVSALGKQ
                     ANHVPTAAQCDSCHTGFVAFAPANMNHGATSAPVAAGNCATCHGGAYVGQGARAKPAT
                     HIVTTQSCDACHTSTAWLPTSFSHTGVAPGACATCHNGTAARGKPAAGHVPTTAACDT
                     CHTNFVGFAPAVMNHAATTGPVAAANCSTCHSGAYLTINAQVKPATHVATTAQCDTCH
                     TSTSSWATATFNHVAASPPVVGRCATCHNSVTALGKPTTHVPTTAQCDICHSGFSAFR
                     PAPMNHAATTGPIAAGNCSACHNGGYTAVNARAKPATHIPTVGQCDSCHSNTSAWAPA
                     TMNHAGTAGQCSSCHSGAYLAQNALAKPPTHIATTAQCDSCHNSTTSWATATFNHGTA
                     SPPVAGRCASCHGISALGKPPTHVPTTAQCDTCHKNFTAFAPATMSHSATTGPLAAGN
                     CASCHGGAYVSAGAVAKTATHLVTTQSCDVCHTTTAWKPTSFAHTGVTPGSCASCHNG
                     TTAVGKPGVHIPTTLACDACHRTSGWLPLITPFAHTGVAAGSCASCHSPSFPNITVKP
                     TSHIPTSAACDACHHSFTAWLPVTYNHVGIAANSCQTCHGGAYANITVKPANHVPTTS
                     PAGSVGNECSLCHTSTTNWLTGRMNHGTMQTGCRLCHLTGAPYQGTMDKKSTTHEQMG
                     TKDCSSSGCHRPLGTRGSPYTRWD"
     gene            complement(5043..6872)
                     /locus_tag="VIS73_01650"
     CDS             complement(5043..6872)
                     /locus_tag="VIS73_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEY5761892.1"
                     /translation="MPAFAQSVEKLVMPGEVISGHVEYEHECGKCHQRFNKAAQNGLC
                     LDCHKDTARDVETRTRFHGRLEGKECRACHTDHKGRDADVAPLNAETFDHDRTEFRVA
                     NAHAAPEVKCEACHKPDKKYREAPRACNACHAKIDQEKGHQGALGKQCEKCHDDRNWT
                     DTTFDHEKTKFRLKGGKHFEVKCSACHSDKRYQKTPLTCIGCHEKDEREKGHKGWYGN
                     KCESCHNDRGWKEIDFDHDSVKRYKLRGKHRKVECDACHVPGKGLPAGKGTIYQQKLQ
                     LTCVGCHREDDRKKGHQGALGDKCDSCHTEREWKTSTFDHDKSDFPLEDKHRDAKCDS
                     CHKGGVTAAGAVARKGKQVRLRDDRFKLDTTCISCHRKDDDEKGHKGRYGKKCETCHD
                     AKDWKRARFDHDRLTKYPLKYKHRQVKCDDCHLPAKGNIYDKRLDDSCHSCHEKDDNE
                     KGHKGQLGKQCESCHSEKRWDDAPYDHDKSRFPLVGGHLGVKCKECHETPAYQDAPEA
                     CQGCHEKDNKHPRRYGEECKTCHYARSWKTWDFDHGQTEYRLDGAHLKIDCQTCHSDS
                     HRDPAKPGRACVACHLKDDIHERRFSLQCQRCHVTADWKEVKP"
     gene            complement(6943..7926)
                     /locus_tag="VIS73_01655"
     CDS             complement(6943..7926)
                     /locus_tag="VIS73_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761893.1"
                     /translation="MAVVTPLKRRASDQSSNNSRQDPQIAELAARRRAQLAASNNERA
                     AAKRQQVLTTRLAVAAAFVCLAVLAVIVATQGIYEPGDDFGYTLGLIGGLLLLSQMVY
                     PLRKRIPLLARLGMMNSWFRYHMLIGVAAPLLILFHSTFKTGSTNGSIAMYTMLLVAA
                     SGLVGRFFYVRVNHGLYGRHRTLGDVTAALQSSLNGVQSVFAIRADLEPRLMAFHRWA
                     FESNLPLWRRAWRFATVRYRGRRLNRRLRKDAKRALRRRRRDDHVDKAELMLSYQLAK
                     QQFDRFIDVVVDASQFRLWERMLSVWHLIHIPFIYLLLFSGLVHVAAVHMY"
     regulatory      complement(7937..8035)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            8329..8628
                     /locus_tag="VIS73_01660"
     CDS             8329..8628
                     /locus_tag="VIS73_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761894.1"
                     /translation="MIELPAGTVLRVKLDQRFDANGNVVRDRVDIRTVQPDGTKIRIR
                     IRAPEIEVNDLEIKNGMVSRQRGRDAQRPEDRNDRVARGDRPERIERRGGNSGEG"
     gene            complement(8734..9081)
                     /locus_tag="VIS73_01665"
     CDS             complement(8734..9081)
                     /locus_tag="VIS73_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEY5761895.1"
                     /translation="MPNITFSSEKMRKDLTVYAAAGDRKTILGVAKTHGVAIDCECEK
                     GECGSCTVEVIHIGNKPPMGVHVTEKEKLTLLTSGKLKKADFEELEILDVAPKHRLAC
                     QYMLLDEDIVVKF"
     gene            complement(9093..>10403)
                     /gene="nifK"
                     /locus_tag="VIS73_01670"
     CDS             complement(9093..>10403)
                     /gene="nifK"
                     /locus_tag="VIS73_01670"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein subunit
                     beta"
                     /protein_id="HEY5761896.1"
                     /translation="AVLCAAGFDGSLPYVHGSQGCVAYFRSHFNRHFKEPTSCVSDSM
                     TEDAAVFGGLNNMVDGLANSYNLYKPKMIGVSTTCMAEVIGDDLDAFIKTAKQKGSIP
                     EDFRVAFAHTPSFVGSHVTGYDNMLKGFLVNFWEGKERVETAAPAININGGFDGYCVG
                     NNRELKRMMKEMGVEATIISDPSDVFDTPTDGEFRMFSGGTTAEEMEAALNAKGTVFM
                     QGVSAVKSAEYVGKAGQKTVSLHCPIGVAGTDALLMAVSELTGKPIPESLELERGRLV
                     DAIADSQAHLHGKKFALFGDPDMLLGLVSFLLELGAEPVHVLCTNATKDFTAKMEELL
                     AASPFGQGAKVWAGKDLWHLRSLLATEPVDFLIGSSHGKYLERDTGTPLIRIGFPIFD
                     RHHHHRSPIWGYQGGMNLLVKILDKIFDVLDAQTADTASFDLVR"
BASE COUNT         1579 a         3226 c         3641 g         1957 t
ORIGIN      
        1 aggcgcggaa ccgtcaatac gatgctccgc ccgtcggcac cgggcttgac cgtgtagcgc
       61 gtcggctgcg agaacgtcag caacataccg ttacgcagct cgggaaacac cgccttcacg
      121 accggcacac cggcaaccac cggcgaacgg acggtgtcct gcatcagttg gtcttcggtg
      181 acgccgctgg tcagcagcct gagaaagacc cgagcctctc gcacctcgcc gcccggaccg
      241 tgacgcaggt actggatgcg gtgcgagaag cggacgacga tctcggcctc gcgttcgccg
      301 gcgacgacgt cgatgcggtc gaagacctgc gcctgcgcgg tcgcactgca caggagcagc
      361 agcgcgacga tgccgcgcag acacccgccg agccgcgctt gaaaacgaat caagtccatc
      421 aatcccacct cgtatagggc gaaccgcgag tgccgagcgg acggtggcag ccgctcgacg
      481 agcagtcctt cgttcccatc tgctcatggg tggtcgactt cttgtccatc gtcccctggt
      541 agggggcgcc ggtcaggtgg cagaggcggc agccggtctg catcgtcccg tggttcatcc
      601 ggccggtcag ccagttggtg gtgctggtgt ggcacagcga gcactcgttg ccgacgctgc
      661 cggccggcga agtggtcggc acgtggttcg ccggcttgac cgtgatgttg gcgtaggcgc
      721 cgccgtggca ggtctggcag gaattggcag cgatcccgac gtggttgtag gtgaccggca
      781 gccaggcggt aaagctatgg tggcaggcgt cgcaggcggc gctggtcgga atgtggctgg
      841 ttggcttgac cgtaatgttc gggaacgacg ggctgtggca gctggcgcag ctgccggcgg
      901 cgacgccggt atgcgcaaac ggcgtgatca gcggcagcca gcccgaagtg cggtggcagg
      961 cgtcgcaggc cagcgttgtc gggatgtgca cgccgggctt gccgacagct gtggtgccgt
     1021 tgtggcagct ggcgcagctg cccggcgtca cgccggtgtg cgcgaaacta gttggcttcc
     1081 acgccgtggt cgtatggcag acgtcgcagg actgggtcgt caccaggtgc gttgcggtct
     1141 tggcgaccgc gcccgccgag acgtaggcgc cgccgtggca gctggcgcag ttgcctgccg
     1201 ccagcggtcc ggtcgtcgca ctgtggctca tcgtcgccgg cgcgaaggcg gtgaagttct
     1261 tgtggcaggt atcgcactga gcggtcgtcg gcacgtgcgt cggcggcttg ccgagcgcgc
     1321 tgatgccatg gcagctcgcg cagcggccgg caaccggcgg gctcgccgtg ccgtgattga
     1381 acgtggccgt tgcccagctg gtcgtggagt tatggcagct gtcgcactgc gccgtggtgg
     1441 cgatgtgcgt tggcggcttg gccagcgcgt tctgcgccag ataggcgccg ctgtggcagc
     1501 tcgaacactg gccggcggtg cctgcgtggt tcatcgtcgc cggtgcccag gcgctggtgt
     1561 tgctgtgaca gctgtcgcac tgcccgacgg tcggaatatg cgtcgccggc ttggcgcgcg
     1621 cgttcaccgc cgtgtagccg ccattgtggc aggccgagca gttgcctgcg gcaatcggcc
     1681 cggtcgtcgc tgcgtggttc atcggtgccg gacggaacgc cgagaaaccg ctatggcaga
     1741 tgtcgcactg ggcggtggtc ggcacgtgcg tggtcggctt gccgagcgcg gtgacgctgt
     1801 tgtggcaggt cgcgcatcgc ccgaccaccg gtggcgaggc ggcgacgtgg ttgaaggtcg
     1861 ccgtcgccca gctgctggtc gaggtgtggc aggtgtcgca ctgcgcggtg gtggcgacgt
     1921 gcgtcgccgg cttgacctgc gcgttgatcg tcaggtaagc gcccgaatgg caggtcgagc
     1981 agttggcggc ggccaccggc ccagtggtcg cggcgtggtt cataaccgcc ggtgcgaagc
     2041 caacgaagtt ggtgtggcag gtgtcgcagg ctgcagtcgt cggaacgtgc ccggccgccg
     2101 gcttgccgcg cgcggccgtg ccattgtggc aggtcgcgca ggcgccgggc gcgacaccgg
     2161 tgtggctgaa gctggtcggc agccaggccg tgctcgtatg gcaggcgtcg caggactgcg
     2221 tggtgacgat gtgcgtagcc ggcttggcac gggcgccctg cccgacgtag gcgccgccgt
     2281 ggcaggtggc gcagttgccg gcggcgaccg gcgcgctggt cgcgccgtgg ttcatgttcg
     2341 ccggcgcgaa ggcaacgaac cccgtgtggc agctgtcgca ctgcgccgcg gttggcacgt
     2401 ggttggcctg cttgccgagc gcactgacgc cgtgacacga agcgcagcgg ccggcaaccg
     2461 gcgggctcgc cgtcgcgtgg ttgaaggtcg ccgtcgccca ggaagtggtc gactgatggc
     2521 agctgtcgca ctgcgccgtg gtggcgacgt gggtcgccgg cttggccagc gcgttctggc
     2581 tgacgtaggc gccgccatgg caggtgctgc agttgccggc ggcgactggc gcggcggtgg
     2641 cggcgtgatt catcctcccc ggcgcgaagg acaggaagcc attcacgtgg cagacgtcgc
     2701 actgaacgct ggtaggcacg tgcgaggtcg acttggccag cgcaccgacc gccgtgtggc
     2761 tgccgccgtg gcaactctga cactgcccgg ccagcccggc gtggttcatc gtcgccggca
     2821 caaaggccgt gtaattggta tggcagttgt cgcactgcag gccagtcggg atgtgcgctc
     2881 ccgacttcgc cggtgcgctg gtgctgccgt tgtggcaggt tgagcaggta ccgacggcgc
     2941 tcgggccgtg ggtgaacgcc gcgcctgtcc agctgctggt cgagcggtgg caggtgtcgc
     3001 actgcgccgt cgttggcacg tggttacccg gtttggcgag cgcaccggcg aagagatacg
     3061 taccgttgtg gcaactggcg cactggcccg tggtggcagc gtggttcatc gtcgccggcg
     3121 cgaaggccgt gaagttcccg tggcagctat cgcactggac ggtggtcggc acatggttgg
     3181 tcggcttgcc gagcgcgttg actccatggc aggacgagca gcgcccggcg accgctggca
     3241 ccgctgtcgc gtggttgaag gtggccgagg cccaggtggt cgtcgacttg tggcagctgt
     3301 cgcactgcgc agtggtggcg acgtgcgtcg gcggcttggc ctgcgcattc atcgccaggt
     3361 aggtaccgct gtggcatgcc gagcagaggc cggtggttcc ggcgtggttc atcgtcgccg
     3421 gccgccaggc ggtgaagccg cccgtgtggc agctgtcaca ggaggctgag gtcgggatgt
     3481 ggttcgccgg ccggccgtcg gcgttggcga atgcgtagtt gccgttgtgg cagctggcgc
     3541 actgccccgc cgtcccggcg tggttcatgc gtgccggacg gaaggcggcg aagccgctgt
     3601 ggcaactgtc gcactgcgca ctggtcggta cgtgcgtggt cggcttgccg agcgcattga
     3661 cgctgttgtg gcagctcgag cagcgaccgg cgaccgccgg cgtcgccgac gaatggtcga
     3721 agatccgcgt cgcccaggtc gtcgtcgact tgtggcaggt atcgcactgg gcggtggtgg
     3781 cgacgtgcgt cgcgtgcttg acctgggcgt tttgcgccag gaatccgccg ctatggcagc
     3841 cgatgcactt gccggcggtg ccggcgtggt tcatcgccgc cggcgtccag ccggtcgtcg
     3901 aggcatggca ggcgtcacat tggccggtgg tgggaatgtg cgagaccggc ttggtgcgcg
     3961 cgccggcgct gacgtaggtg ccgccgtggc agcccacgca ctgcgcagcg cttgaaaaat
     4021 ggttcatctg cgccggtttg aaggcggtga agccgacatg gcaaaggtcg cactgcaggt
     4081 tggtcggtac gtgcgaagac gatttgccga gcgccgaggt gccgtggcag ctcgagcaga
     4141 taccagtcgc cggcggcgac agcgagccgt ggtcgaagct ggcggtcgcc caggtggtag
     4201 tcgaagaatg gcagctgtcg cactgctcgc tggtcgggat gtggctggcc ggcttggcct
     4261 gcgcgttctg cgccgtgtag ttgccgttgt ggcacatcgc gcagttgccc gcggccaccg
     4321 gcccgctcat cgccgaatgc agcgtcgccg tgctggtagt cgtcggcgca aaggctgagc
     4381 cgatcgcgtg gcattgctcg cagctggtcg aggtcggaat gtggcggatc gatcgcccgg
     4441 tggcgcgaat gccgttgtgg caggactggc acgaacccgg tgtcgccaat gcgtgcgtgt
     4501 agccggccgg cgtccacgcc gtcgagcggt ggcaggaatc acaggcggcg ttggtgacga
     4561 tgtgattggc cggcttgccg tgcgccgacg agccgttatg gcagatcgcg caactgccga
     4621 cgctgacgcc ctgagccggc tggtggcgga aggtcgagat cgaccagttg ctggtcagat
     4681 ggcaggcgtc acaggcagtg gtggtcggta cgtgcgaggg cgtcttccct ggcgcccggc
     4741 cacccgagcg gtggcagccg gcgcagtcgc gcggcgtgcc gcggaacaga ccctgccggt
     4801 gacaggattc gcaacgttcg cgcacgtgcg cgccgtcgag cgtgtagccg acggtcgcgt
     4861 ggttgaacga cccgctgccc gagatgttct gcgcggcggc cggcacggcc gccagtgcca
     4921 tcaggatgac caggctgcgg gcgagcgcgc ctgcgattct cggcaaggtt ctcatttcgg
     4981 ctgctccttc cgtactggcg cgaagcaagg cttcgcgccg gggatcttgg tatcgcttcg
     5041 ggtcatggct tgacctcttt ccagtccgcg gtgacgtgac agcgctggca ttgcaggctg
     5101 aagcgtcgtt cgtggatgtc gtctttcagg tgacaggcaa cgcaggcgcg tccgggcttc
     5161 gccggatcgc gatggctgtc gctgtggcag gtctgacagt cgatcttgag gtgggcgccg
     5221 tcgaggcggt attcggtttg cccatggtcg aaatcccatg tcttccacga gcgcgcgtag
     5281 tggcaggtct tgcattcctc gccgtagcgg cgcggatgct tgttgtcctt ttcgtggcaa
     5341 ccctggcaag cctcgggcgc gtcctgatag gccggtgttt cgtggcattc cttgcacttg
     5401 acgccgaggt ggccgccgac cagtggaaag cgcgacttgt cgtggtcgta gggcgcgtcg
     5461 tcccagcgct tctcgctgtg gcagctctcg cactgcttcc cgagctggcc cttgtgcccc
     5521 ttctcgttgt cgtccttctc gtgacagctg tggcagctat cgtcgagacg cttgtcgtag
     5581 atgttgccct tggccggcag gtggcagtcg tcgcacttga cctgccgatg cttgtacttc
     5641 agcgggtact tggtcaggcg gtcgtgatcg aagcgcgcgc gcttccagtc cttcgcgtcg
     5701 tggcaggtct cgcacttctt gccgtagcgg cccttgtgcc ccttctcgtc gtcgtccttg
     5761 cggtggcagc tgatgcaggt cgtgtcgagc ttgaaacggt cgtcgcgcag ccggacctgc
     5821 ttccctttgc gggcaacagc gccagcggcg gtgacgccgc ccttgtggca gctgtcgcac
     5881 ttggcgtcgc ggtgcttgtc ctcgagcggg aagtcgctct tgtcgtggtc gaaggtcgag
     5941 gtcttccact cgcgctccgt gtggcatgaa tcgcacttgt cgccgagcgc gccctggtgg
     6001 cccttcttgc ggtcgtcctc gcggtggcag ccgacacagg tcagctgtag cttctgctgg
     6061 tagatcgttc ccttgcctgc gggcaacccc ttgcccggta cgtggcaggc atcgcactcg
     6121 accttgcggt gcttgccccg cagcttgtag cgcttgaccg aatcgtggtc gaagtcgatt
     6181 tccttccagc cacggtcgtt gtggcaggac tcgcacttgt tgccgtacca gcccttgtgg
     6241 cccttctcgc gctcgtcctt ctcgtggcag ccgatgcagg tcagcggcgt cttctggtag
     6301 cgcttgtcgc tgtggcaggc cgaacacttc acctcgaagt gcttgccacc cttgagacgg
     6361 aacttggtct tctcgtggtc gaaggtggta tcagtccagt tgcggtcgtc gtggcatttc
     6421 tcgcactgct tgccgagtgc cccctgatgg cccttctcct gatcgatctt ggcgtggcaa
     6481 gcgttgcacg cgcgcggcgc ctcgcggtac ttcttgtccg gcttatggca ggcctcgcac
     6541 ttcacctcgg gtgcggcatg cgcattggcg acgcggaact cggtccggtc gtggtcgaag
     6601 gtctcggcgt tgagcggagc gacgtcggca tcacgcccct tgtgatctgt gtggcaggcg
     6661 cggcactcct taccttcaag gcgcccatga aaacgcgtgc gggtctcgac gtcgcgcgca
     6721 gtgtccttgt ggcagtcgag gcacaacccg ttctgcgccg ccttgttgaa gcgctgatgg
     6781 cacttgccgc attcgtgttc gtactcgacg tggccgctga tgacctcgcc gggcatcacc
     6841 agcttctcga ccgactgcgc gaatgccggc acggccgttc cggcgaggcc ggacagcagc
     6901 gagataacag ccagcagccg gagtccggcc ccgactgccc gcttagtaca tatgaacggc
     6961 agcgacatgg accaggccgc tgaacagcag caggtagatg aatggaatgt ggatgaggtg
     7021 ccagaccgaa agcattcgct cccacagccg gaactgcgag gcatcgacca ccacatcgat
     7081 gaagcggtcg aactgctgtt tcgccagctg atagctcagc atcagctcgg ccttgtcgac
     7141 gtggtcgtcg cgccggcggc ggcgcagcgc gcgcttggca tccttgcgca aacggcggtt
     7201 gagccggcgc ccgcgatagc gcaccgtggc gaagcgccag gcgcggcgcc acagtggcag
     7261 gttggattcg aaggcccagc ggtggaacgc catcagccgc ggctcgaggt cggcgcgaat
     7321 cgcgaacacc gactgcacgc cgttgaggct ggactgaagc gccgccgtca cgtcgccgag
     7381 cgtgcgatga cgcccgtaga ggccgtgatt gacgcggacg tagaaaaaac ggccgaccag
     7441 cccgctggcg gcaaccagca gcatcgtgta catcgcgatg ctgccgttgg tcgaaccggt
     7501 cttgaaggtc gaatgaaaca ggatcagcag cggtgccgca accccgatca gcatgtggta
     7561 gcggaaccag ctgttcatca tgcccagccg ggcgagcagc ggaatccgct tgcgcagcgg
     7621 atagaccatc tggcttagca acaagagccc gccgatgagg ccgagcgtgt atccaaaatc
     7681 gtcgccaggc tcgtagatgc cctgcgtcgc gacgatgacc gcaagcacgg ccaggcagac
     7741 gaacgccgcc gcaacggcga ggcgggtcgt cagtacctgc tggcgcttgg ccgcggcgcg
     7801 ctcgttgtta gacgcggcaa gctgcgcgcg acggcgcgcc gcgagctcgg cgatctgcgg
     7861 gtcttgcctg gaattgttgc tggattggtc gcttgcgcga cgctttaacg gtgttacgac
     7921 ggccatggca gctgctctcg gcagtcccgc tgtcatgggg agcccgtgct gcagcaccct
     7981 ttagaccggt gactttgcgc ccccgccctt tcggacggga ttacctttat cggaaccgag
     8041 caagcgatct gccggcggtg gaatcgcacc gccgagtctc gcgcctgctc gcgccgcagg
     8101 ttatgaccct gctttaggtg tgcattattg cctcagccgc atcattggtc aacttttctg
     8161 tgaactaggc ccttgcgttc tcccaccacg tctgctaggt cgcggccatt gccaatcatt
     8221 gcgcgaagcg gcagttatcc gcggggatga cgaatcgcga ccgatcccga acgccagtac
     8281 gggacgcgcc cgccggcgat gaccctgccc gatcgacgta gaggagaaat gatcgagcta
     8341 cccgccggca cggtccttcg cgtcaagctc gaccagcgct tcgacgccaa cggcaacgtt
     8401 gttcgcgatc gcgtcgacat ccgcaccgtc cagcccgacg gtacaaagat ccgcatccgc
     8461 atccgtgcgc ctgagatcga ggtcaacgac ctcgagatca agaacggaat ggtcagccgc
     8521 caacgcggcc gcgacgcgca acgccccgag gaccgcaatg atcgcgtcgc gcgcggcgac
     8581 cggcccgagc gcatcgaacg gcgcggcggc aacagcggcg aaggctgaac gccgtcgagt
     8641 accagcgccg aagcactgcg ttaccgccta ggaaaaaacg gcccgcatcg ctgcgggccg
     8701 aagccattcg acgtcaatcg aacagggagg agcctagaac ttcacgacga tgtcttcgtc
     8761 gagcagcatg tactggcagg ccagccggtg cttgggggcg acgtcgagaa tttcgagttc
     8821 ctcgaaatcg gccttcttga gcttgccgga agtcagcagc gtgagctttt ccttctcggt
     8881 gacgtgcacc cccatcggcg gcttgttgcc gatgtggatc acctcaaccg tgcaggaacc
     8941 gcactcgcct ttttcgcact cgcagtcgat cgcgacgccg tgggtcttgg cgacgccgag
     9001 aatcgtcttg cgatcgccgg cggccgcgta gaccgtcagg tccttgcgca tcttttcgga
     9061 gctgaaggtg atgttgggca tgtcgtttct ccttagcgca cgaggtcgaa actcgccgta
     9121 tcggcggtct gcgcatcgag cacgtcgaag atcttgtcga ggatcttgac cagcaagttc
     9181 atgcccccct ggtagcccca gattggcgac cggtggtggt gatgccggtc gaagatcggg
     9241 aagccgatgc ggatcagcgg cgtgccggtg tcgcgctcga ggtacttgcc gtgagagcta
     9301 ccgatcagga aatcgacggg ctcggtggcg agcagagaac gcaggtgcca caggtccttg
     9361 ccagcccaca ccttggcgcc ctgcccgaat ggacttgccg cgagcaactc ttccatcttc
     9421 gccgtgaagt ccttggtggc gttggtacag aggacgtgca ccggctcggc accgagttcg
     9481 agcaggaagg acaccaggcc cagcagcatg tccggatcgc cgaaaagcgc gaacttcttg
     9541 ccgtgcagat gcgcctgcga gtcggcgatc gcgtcgacca ggcggccgcg ctcgagctcg
     9601 agcgattccg gaataggctt gccggtcagc tctgaaaccg ccatcagcag ggcatcggta
     9661 ccggcgacgc cgatcgggca gtgcagcgaa accgtcttct ggccagcctt cccgacgtac
     9721 tcggccgact tcaccgccga gacgccctgc atgaagacgg tgcccttggc gttgagtgcc
     9781 gcttccatct cttccgccgt agtaccgccc gagaacatgc ggaattcccc gtcggtcggg
     9841 gtatcgaaca cgtcggacgg gtcagagatg atcgtcgcct cgacgcccat ttccttcatc
     9901 atccgcttca gttcacggtt gttgccgacg caatagccgt cgaaaccgcc gttgatattg
     9961 attgccggag ccgccgtctc gacgcgctcc ttgccttccc agaaattgac caggaagccc
    10021 ttaagcatgt tgtcgtagcc ggtgacatgg ctacccacga agctcggcgt gtgcgcgaag
    10081 gcgacccgga agtcctcggg aatggaaccc ttctgcttgg cggtcttgat gaaggcatcg
    10141 aggtcgtcac cgatgacttc cgccatgcag gtggtcgaca cgccgatcat cttcggcttg
    10201 tacaggttgt acgagttcgc aaggccgtcg accatgttgt tgaggccacc gaacaccgcg
    10261 gcatcttcgg tcatcgaatc ggagacacag gaagtcggct ccttgaagtg acggttgaag
    10321 tgcgagcgga agtaggcgac gcaaccctgc gacccgtgga cgtacggcag cgaaccgtcg
    10381 aaaccggcgg cgcagagcac ggc
//
LOCUS       DATDAA010000048         2992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_213213,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000048 DATDAA010000000
VERSION     DATDAA010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213213"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VIS73_01675"
     CDS             complement(<1..1080)
                     /locus_tag="VIS73_01675"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY5761897.1"
                     /translation="MLHDGRSVRVAVVGGSRIPFCRSHSVYRKCTNQDLMTAALQGLV
                     NKLDLRGQTMGDVALGAVIKHSRDWNLARESVIDSGLTLRTPGMDLQRACGTSIEAAI
                     LIANKIALGQIDCGIAGGTDTVSDAPIVFRDEFRHILLDVHAARSAMQRLMPWFRLRP
                     RHFAPEFPGVLEPRTGLSMGESMELTAKQWDVQREHQDQLALASHVKAAAAYDAGWYD
                     DLVVPFMDAEEDNNIRRDTTFEKLQKLKPVFDKSAEGSLTAGNSTPLTDGAAAVLLAN
                     EDWARKKNLPIMAYLTFAKVSAINFINEEGLLMAPAYAVSDMLKQADLRLQDFDFYEI
                     HEAFAAQVIATLKAWQSEEFCREKLG"
     gene            1238..2611
                     /locus_tag="VIS73_01680"
     CDS             1238..2611
                     /locus_tag="VIS73_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY5761898.1"
                     /translation="MQQTVRRFGREASALLGIGWPLIINNLSVAGMQFTDAVMAGRLG
                     AESLAAVAVGGSVWFLGFALVLGVLMAISPIASRHHGAGDEALIGRYTRQGLYLGAFL
                     AVPIILFSWFLAAPALAAIGIDVAFRDMTVGYVKMISLGAPGMFIFLALRFTTEGIGH
                     TRPIMYTSILALACNVFLNWVLMYGNLGAPAMGAVGCGLASGISMWIIAIVLSAYMFS
                     SKVYDPLDLFSRLSPMRPSALKEIVFLGVPIAITITAEAGLFNAVSILMGTLGPHITA
                     AHQIAINFASTMFMVPLALNSAITVRVGYALGKGDLEYARFAGASGVVLCALFMACSA
                     TFLLLFRDAVVSIYTQDAAVKGIAISLLLMAAIFQVADGIQIGTAGALRGYKDTRVPM
                     AINIFAYWVIAFPLAYLAAVTYKLPPNYIWGGFVVGLTVAAIMLTRRYARISRDAIDE
                     LHYLNRG"
     gene            complement(2664..2969)
                     /locus_tag="VIS73_01685"
     CDS             complement(2664..2969)
                     /locus_tag="VIS73_01685"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEY5761899.1"
                     /translation="MQKKDRGLTPIQRAFFYMPLQHAESCKVQAKSVELFSRLAKAVS
                     PTFRETFLTIVQFAELHRDIIEQYGRFPHRNVLLGRENTPEEDEYLAGDAPDFGQSG"
BASE COUNT          487 a          943 c          942 g          620 t
ORIGIN      
        1 gccgagtttc tccctgcaga attcttcgga ctgccaggct ttgagcgtgg caatcacctg
       61 cgccgcgaag gcttcgtgga tctcgtagaa atcgaagtcc tgcagcctca ggtccgcctg
      121 cttcagcatg tccgagaccg cgtacgcggg cgccatcagc aagccttctt cgttgatgaa
      181 attgatggcc gacactttcg cgaacgtgag atatgccatt atcggcaggt tcttcttgcg
      241 cgcccagtcc tcgtttgcga gcaggaccgc agcggcgccg tccgtgagtg gggtgctgtt
      301 gccggcagtc agcgagccct cggcggactt gtcgaatacg ggcttcagct tctgcagctt
      361 ctcgaaggtg gtgtcgcggc gaatgttgtt gtcctcctcc gcatccatga acggcaccac
      421 gagatcgtca taccagccgg cgtcgtaggc ggccgcggcc tttacgtggc tcgcgagcgc
      481 gagctggtcc tggtgttcgc gctgcacgtc ccactgcttg gcggtgagtt ccatgctctc
      541 gcccatggac aggccggtgc gcggctcgag gaccccgggg aattccggcg cgaagtgcct
      601 cgggcgcagg cggaaccacg gcattaaacg ctgcatcgcg gagcgtgcgg cgtgaacatc
      661 gaggaggatg tggcggaact cgtcacgaaa cacgatgggc gcatcgctga cggtatcggt
      721 accgccggcg atgccgcagt cgatctggcc gagtgcgatc ttgttggcga tcaggatcgc
      781 cgcttcgatg ctcgtgccgc aggcgcgctg caggtccatg cccggggtcc gcaatgtcag
      841 cccggagtcg atcacgctct cgcgggcgag attccagtcg cgcgaatgct tgatgaccgc
      901 acccagcgcg acgtcgccca tggtctgccc gcgcaggtcg agcttgttga cgagtccctg
      961 cagggccgcc gtcatcaggt cctggttggt gcacttcctg tagacggaat gcgagcgaca
     1021 gaaaggaata cgggagccgc cgacaacggc gacgcggacg gatcgaccat cgtgcaacat
     1081 attgcctccg aagccagagt ttcacacgag cgcgaagcaa ctttacacta tcgaccgagt
     1141 ggaacgtaga agggtctccc gttttaggtg cggtgccccg gctccgccac ctcaccaatt
     1201 cccggccggg tgattccggc agccacggca gatgcagatg cagcagacag tccgacgttt
     1261 cgggcgcgag gccagcgcgc ttctcggcat cgggtggccg ttgatcatca acaatctctc
     1321 ggtcgcgggc atgcagttca ccgatgcggt gatggccggc cggctcggtg ccgagtcgct
     1381 ggcggccgtg gcggtcggcg gcagcgtctg gtttctcggg tttgcactcg tactgggcgt
     1441 gctgatggcc atatcgccga tcgcctcgcg tcatcatggt gcgggggacg aggccctgat
     1501 cggccgctac acgcgtcagg gtctttatct cggggccttc ctcgccgtac cgatcatcct
     1561 gttttcctgg ttcctcgccg cgccggccct cgccgcgatc ggcatcgacg ttgcgttccg
     1621 cgacatgacc gtcggttacg tgaagatgat ttcgctcggt gcgccgggga tgttcatttt
     1681 tctcgcgcta cgattcacga ccgaaggcat cggccatacc cggccaatca tgtacacatc
     1741 cattcttgcg ctcgcctgca acgtgttcct gaactgggtg ctgatgtacg ggaacctcgg
     1801 cgcgccggcg atgggcgccg tgggctgcgg cctcgcgagc ggcatctcga tgtggatcat
     1861 tgcgatcgtg ctgtcggcct atatgttctc gagcaaggtg tacgacccgc tggatctgtt
     1921 ctcgcggtta tcgccgatgc gcccaagtgc gctgaaggag atcgtcttcc tcggcgtgcc
     1981 gatcgcgatc acgatcaccg ccgaagccgg cctgttcaat gccgtgtcca tcctgatggg
     2041 taccctgggc ccgcacatta ccgccgcgca ccagatcgca atcaacttcg catccacgat
     2101 gttcatggtg ccgctggccc tcaactcggc gattaccgtc cgcgtcggct atgccctggg
     2161 caagggggac ctcgaatacg cacgcttcgc cggggcgtcc ggggtcgtgt tatgcgccct
     2221 gttcatggcc tgttctgcga cgttcctgct gttgttccgc gacgcggtcg tcagtatcta
     2281 cacgcaggac gcggccgtga agggcatcgc gatttccctg ctgctgatgg cggcgatctt
     2341 ccaggtggcg gacggcatcc agatcggcac cgccggcgcg ctgcgaggct acaaggacac
     2401 ccgggtgccg atggcgatca acatcttcgc ctactgggtc atcgcgttcc cgctcgccta
     2461 cctggccgcg gtcacgtaca aactgccgcc gaactacatc tggggcgggt tcgttgtcgg
     2521 cctgaccgtc gcggccatca tgctgacccg gcgctatgcc cgcatctcgc gagacgccat
     2581 cgacgaactc cactacctga acaggggata gggactgaaa gtgccgtgtg cgacgaccgt
     2641 ctggcaccgt caatcactgc ttgttaaccg gattggccga aatccggtgc gtcgccggcg
     2701 aggtattcgt cctcttccgg cgtgttctca cggccgagca gcacgttcct gtgcgggaag
     2761 cgcccgtatt gctcgatgat atcgcggtgc agttccgcaa attgcacgat ggtcaggaag
     2821 gtctccctga aagtaggcga taccgccttg gccaagcggc tgaaaagctc gacggatttt
     2881 gcctgtacct tgcacgattc ggcatgctgc agcggcatgt agaagaacgc ccgctgtatc
     2941 ggcgtgagtc cgcggtcctt cttctgcatc gcgccctcga cacacagctt ca
//
LOCUS       DATDAA010000049         2119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_178033,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000049 DATDAA010000000
VERSION     DATDAA010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178033"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VIS73_01690"
     CDS             complement(<1..635)
                     /locus_tag="VIS73_01690"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761900.1"
                     /translation="MQEPFDIAADLDTPVSAFLKLAPLRPRFLLESVEGGERLARYSF
                     LGFGDALEVRMDANGLSVDGESRPRPATRDEYLGALRDALARAPRPQPEVEGLPFSGG
                     LVGVSGYDVVRLFERLPRDTEPQSSVPDAAFSAPTSFLVFDHLTRRVALLHDGPDAER
                     QALRREVIDRLRGPVPAARTDVQISPATASLSEAEYTDRVNACKEYIAAGD"
     gene            complement(832..>2119)
                     /locus_tag="VIS73_01695"
     CDS             complement(832..>2119)
                     /locus_tag="VIS73_01695"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY5761901.1"
                     /translation="GQHTFSLFEWVLPVSIIGGVAILFLWLVAPRLLPDRTPLMADTT
                     PRIFSGQLHIRKGGFADGSTLSEVLAKADGQMRVDKIQRSESLFLAKLPQVKLQPGDR
                     LFVKDSPENLKHYEQILGATLYNAEDEEHPVDDEFPLKAGGQQLAEVVVTRGSPLHLR
                     TLAAARFSGSYGLMPLALHRARAPSSQITGDLNTIRLRAGDVLLVQGSGEAINHLKDS
                     GSMLVLDGTTDLPQTHHAKRALTIMALVVAAAALGILPIAASALVGLGFMLATGCLSW
                     RDAAGALSIPVIMIIVASLALGKALLGTGMADYIAMGFVSMTSALPPGMILSAFMLLM
                     ALLTNIVSNNAAAAIGTPIAIGIAQQLGVPADPFILATLFGANMSFATPYGYQTNLLI
                     LSAGGYKFTDFLRVGIPLTIIMWIGFSLLLPVLYSL"
BASE COUNT          388 a          693 c          685 g          353 t
ORIGIN      
        1 atgtcgccgg ccgcgatgta ctccttgcag gcgttcaccc ggtctgtgta ctccgcctcg
       61 ctcaggctcg ccgtggccgg tgatatctgc acgtcggtcc tggccgccgg gaccggcccg
      121 cgcaggcggt cgatgacctc gcggcgcagc gcctggcgtt ccgcatccgg cccgtcgtgc
      181 agcagcgcga cgcggcgcgt caggtgatcg aagacgagaa acgaggtcgg cgccgagaaa
      241 gcggcatcgg gaacgctcga ttgcggctcg gtgtcgcgtg gcaatcgctc gaacaggcgc
      301 acgacgtcgt aacccgacac gccaaccagt ccgccggaga acggcagacc ttcgacctcg
      361 ggttgcggac gcggcgcccg tgcgagtgcg tcacgcagtg cgccaaggta ttcgtcgcgc
      421 gtcgccggtc gcggccggct ttcaccgtcg acactgagcc cgttcgcgtc catgcgcact
      481 tcgagcgcgt cgccgaagcc caggaacgaa tagcgcgcga gccgctcacc gccctcgaca
      541 ctctccaaca ggaagcgggg ccgcagcggt gcgagcttca gaaacgccga taccggtgta
      601 tcgaggtcgg cggctatgtc gaatggttcc tgcatggttc atccaaaaaa aaacccatct
      661 ccactctggc ggagacgggc ttctggtttt gctttgctca gctaatagtc ggccagctac
      721 cccctcctag gcgggcagcc accaccagac attgctgtgc cgagcgttca tagcccggag
      781 tattactgtc ccgtcgccct gccgtcaaga acaaggagcc acagcgtcgt tttacaggga
      841 ataaagcacc ggcagcagca gcgagaagcc gatccacatg atgatcgtca gcgggatgcc
      901 cacgcgcagg aagtccgtga acttgtagcc acctgcactc agaatcaaca ggttggtctg
      961 gtagccgtaa ggcgtcgcaa aactcatgtt ggcgccaaac agggtcgcca ggatgaaggg
     1021 atccgccggc acgccgagct gctgtgcgat gccgatggcg ataggcgtgc cgatcgcggc
     1081 cgccgcgtta ttggaaacga tgttagtcag cagcgccatc agcaacatga aggcgctgag
     1141 gatcatcccc ggcggcagcg ccgaggtcat gctgacgaaa cccatggcga tgtagtccgc
     1201 cattccggtc cccagcagcg ccttgccgag cgcgaggctc gcgacgatga tcatgataac
     1261 gggaatcgac agggcgcctg ccgcatcgcg ccagctaagg cagcccgtcg cgagcatgaa
     1321 gccgagtccg accagggcgc tggccgcaat gggcaggatg cccagtgccg ctgccgcgac
     1381 gaccagggcc atgatcgtca gcgcgcgctt ggcgtggtgc gtctgtggca ggtcggtcgt
     1441 gccgtccagc accagcatcg agccgctgtc cttcaggtgg ttgatcgctt cccccgagcc
     1501 ctgcacaagc agcacgtcgc ccgcgcgcag ccggatcgtg ttcaggtcgc cggtaatctg
     1561 tgaactcggc gcacgcgccc ggtgcagtgc gagcggcatg agcccgtagc ttcccgagaa
     1621 ccgcgccgcg gcaagcgtgc gcagatgcag gggcgagccg cgcgtgacca cgacttcagc
     1681 gagttgctgc ccgccggcct tgagcggaaa ctcgtcgtca accgggtgtt cctcgtcctc
     1741 ggcgttgtaa agcgttgcgc cgaggatctg ctcgtagtgc ttgaggttct ccggcgagtc
     1801 cttgacgaac aggcgatcgc ccggctgcag tttcacctgc ggcagcttcg cgaggaacag
     1861 ggactcgctg cgctggatct tgtcgacccg catctgaccg tcagctttcg caagcacctc
     1921 ggacaaggtg ctgccgtcgg cgaatccgcc cttcctgatg tgcagctgcc cgctgaagat
     1981 acgtggcgtc gtatccgcca tgagcggcgt gcggtccggc agcaggcgtg gcgcgacgag
     2041 ccacaggaac aggattgcca cgccgccgat aatcgaaacc ggcagcaccc attcgaacag
     2101 cgagaacgtg tgctgaccc
//
LOCUS       DATDAA010000050         5324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_248179,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000050 DATDAA010000000
VERSION     DATDAA010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248179"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VIS73_01700"
     CDS             complement(<1..291)
                     /locus_tag="VIS73_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761902.1"
                     /translation="MQIVPPKRAAHTYVQTLVTPPDQVFTLLCPVREADWIDGRDPRL
                     MISASGVVEPDCVFVTPSEGTEATWVVTRHEPAAGFVEMLKITPGVTACRLTI"
     gene            complement(407..1216)
                     /locus_tag="VIS73_01705"
     CDS             complement(407..1216)
                     /locus_tag="VIS73_01705"
                     /inference="COORDINATES: protein motif:HMM:NF004794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEY5761903.1"
                     /translation="MKFESIGVQTECAVATIALNRAARANALDATLWRELSAVFDWLD
                     GSEARAAVLCGAGEHFCAGIDLAFLQAVQQQVAALPVAERVPSLEALIRDLQRATGAA
                     ERCRKPVIAAVHGACFGGGVDLIATCDLRFATRDARFSVKEVDLAIVADLGSLQRLPR
                     LVGEGMARELAFTGREFDGEEAVRIGLANEALADREALMEHAQSVARAIAAKSPATVR
                     GIKAAFNYNHSHTVEEGLEHIARHNARALFSSDLYEAMAARQEKRAPKFKD"
     gene            complement(1260..2030)
                     /locus_tag="VIS73_01710"
     CDS             complement(1260..2030)
                     /locus_tag="VIS73_01710"
                     /inference="COORDINATES: protein motif:HMM:NF022317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEY5761904.1"
                     /translation="MADRLRTLDWLRWGLLLPAALVGWYAALVAGMLLLALAERLCPA
                     EAMVSGACVAPWFDSVSTAVFCFSTGLAAALIVLLPTLVAPRARPHVAWVVFGLGSVA
                     AVLMGVTASAYAEMASAVVAGALMAIWLSRRDSAGSEGFFLGGSHVLALHFLFVLFAV
                     FGGLLVLVDLRWACLHLPAVAWSSIVNLAGWTCPLTPLEKELRARDGQAAYDGGFIEH
                     YLGPLVYPLGMPRRMELVAGISIVVWNVIVYGAIWALA"
     gene            complement(2033..2398)
                     /locus_tag="VIS73_01715"
     CDS             complement(2033..2398)
                     /locus_tag="VIS73_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761905.1"
                     /translation="MHCSRLLQRAVDGLAVRRLRRLVGVLSGALLMVSLCLPLTLRAE
                     AVPITEDKARAIALAAAGCDAKRDCVVKDSFTDGQWVFIVSFVHSRDDEGKPRFMPGG
                     FVGITFDAEGKVIDRMPGL"
     gene            complement(2399..2788)
                     /locus_tag="VIS73_01720"
     CDS             complement(2399..2788)
                     /locus_tag="VIS73_01720"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5761906.1"
                     /translation="MTSPEENRRNAIAFYDLMFNQCRPREAIERYTGDTYTQHNPHVA
                     DGKAAFVDYFERMAREYPGKRVSFKRAVAEGDLVVLHCHQVWPTDANRDWAGIDIFRF
                     DEAGKIVEHWDVLQVVPGEASNDNGMF"
     gene            complement(2825..3361)
                     /locus_tag="VIS73_01725"
     CDS             complement(2825..3361)
                     /locus_tag="VIS73_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY5761907.1"
                     /translation="MSLQELARQFSAEGRLEAIVLRPARDAAASQVSEVLAEPGRGLL
                     GDRRATTVRRSAAAQKRDLTLFQAEHLDVLARWCGIDAVDPCRLRRNLVVSGINLIAM
                     RSPFRDFELEWAIGDSVRVVLTGPCDPCSKMEDELGPGGYNALRGHGGMTARIVAGGM
                     LRVGDRIALAAVNPTAAV"
     gene            complement(3467..3931)
                     /locus_tag="VIS73_01730"
     CDS             complement(3467..3931)
                     /locus_tag="VIS73_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761908.1"
                     /translation="MPDSELPLFYQSPWFAVAFIAAAVGIAVLFSVLSGWRSLARRFP
                     ARPDAAGEHFRFASAKMGLVSWFPVSYGGVLAVTVAPEGIAMSICQPFRLLCPAFFLP
                     WSEVLSVKERVAFLSRRIVVSIRDSPVEIALRGPAGKSVAAMFDQVRGAKAE"
     gene            complement(4174..4554)
                     /locus_tag="VIS73_01735"
     CDS             complement(4174..4554)
                     /locus_tag="VIS73_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761909.1"
                     /translation="MPFVHITSLPFDQPFDVPEVVAGISNDFAEATGVGLEHVTATWQ
                     VLLPGYYAVAGITAQCQPEASHPVLVEILAPDFQTPEQIETALLAVAVSIAGRTGIPR
                     QNIFINYRQAHSGMVFDAGELVRW"
     gene            complement(4644..5279)
                     /locus_tag="VIS73_01740"
     CDS             complement(4644..5279)
                     /locus_tag="VIS73_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5761910.1"
                     /translation="MAHCCIDHITITAPTLEAGAESVRQALGVVPQAGGEHPRMATHN
                     LLLRLGDSLFLEVIAPNPASPAPARPRWFALDTLQPGAEPVLSNWVARTADIRASVAA
                     CSEVLGAVEPMSRGALDWLITIPADGSLPLGGIAPALIEWQAAPHPAAKLIDHGLSLV
                     RLELFHPEPERISHLLGSIDFAGPLSVLPSGDRRSARLVAHIDTPQGRRLL"
BASE COUNT          976 a         1840 c         1718 g          790 t
ORIGIN      
        1 gatcgtcaac cggcaggcgg tgacgcccgg cgtgatcttc agcatctcga cgaagcctgc
       61 ggccggctcg tgccgcgtga caacccaggt cgcctcggta ccttcggatg gcgtgacgaa
      121 gacgcagtcg ggttcgacga cacccgacgc cgagatcatc aggcgcgggt cccggccgtc
      181 tatccagtca gcctcgcgca ccgggcacag cagcgtgaac acctggtcgg ggggagtaac
      241 gagtgtctgc acgtaggtgt gtgcggcgcg cttgggcggg acgatctgca ttgggaattc
      301 cttgcgggtt gatcgttgaa accaggatgt tcagtccgca tcacttccgc cgggtttgcg
      361 gttccgttgg tcaccgcggt gtcgccgacg ccgcgttcgc cgcatctcag tccttgaact
      421 tcggcgcccg cttttcctgg cgcgcggcca tcgcttcgta gaggtcggat gaaaacagcg
      481 cgcgcgcatt gtgccgggca atatgctcga ggccttcctc gaccgtatgg ctgtggttgt
      541 agttgaacgc cgccttgatg ccacgcacgg tggccggcga cttggcggcg atggcgcggg
      601 cgactgactg cgcgtgctcc atcagcgctt cgcggtcggc cagcgcctcg ttggcgaggc
      661 cgatgcgcac ggcttcctcg ccgtcgaatt cgcgcccggt aaaggccagt tcgcgggcca
      721 tgccctcgcc taccaagcgt ggcagccgct gcaggctacc aaggtcggcg acgatggcga
      781 ggtcgacctc cttcaccgag aagcgcgcat cgcgcgtcgc gaagcgcagg tcgcaggtgg
      841 cgatcaggtc cacgccgccg ccgaaacagg cgccgtgaac tgccgcgatc accggtttgc
      901 ggcagcgctc ggcggcgccg gtcgctcgct gcagatcacg gatcagcgct tctaggctcg
      961 gtacgcgctc ggcgaccggc agcgccgcca cctgttgctg caccgcctgc aggaacgcga
     1021 ggtcgatgcc ggcgcagaag tgctcgccgg cgccgcagag cacggctgcc cgcgcctcgc
     1081 tgccgtcgag ccagtcgaac accgccgaca gctcgcgcca cagcgtcgcg tcaagtgcgt
     1141 tagcgcgcgc cgcccggttc agcgcgatgg tggcgacggc acactcggtt tggactccga
     1201 tcgattcgaa tttcatgcag actccctacc gcttgcgttg ctgctgcctt tcgatgcgac
     1261 tacgccagtg cccagatcgc accatagacg atgacgttcc acacgacgat ggaaatgccg
     1321 gcgaccagct ccattcgccg gggcatgccc agcggataga ccagcggccc gagatagtgt
     1381 tcgatgaagc cgccgtcgta ggctgcctgg ccgtcgcgcg cccgcagctc cttttccagc
     1441 ggcgtcagcg ggcaggtcca gccggcgagg ttgacgatgg atgaccaggc caccgccggc
     1501 agatgcaagc aggcccagcg aaggtcgacg aggaccagca ggccgccaaa gacggcgaac
     1561 agcacgaaca agaagtgcag cgcgagcacg tggctgccgc caaggaagaa gccctcgctg
     1621 cccgcgctgt cgcgtcgcga cagccagatg gccatcagcg cgccggcgac tacggcgcta
     1681 gccatctccg cgtaggcgct cgcggtcacg cccatcagca cggcggcgac cgagcccaag
     1741 ccgaagacga cccaggccac gtggggccgc gcgcgcggtg cgaccagcgt cggcagcaac
     1801 acgatgagtg ccgccgccag cccggtcgag aaacagaaca cggccgtgga aaccgagtcg
     1861 aaccagggcg ccacgcaagc gccagagacc atcgcttcgg ccgggcacag gcgctcggcc
     1921 agcgccagca gcagcatgcc ggccaccaat gccgcgtacc agccgaccag cgccgccggc
     1981 agcaacagcc cccagcgcag ccaatctagc gttctgaggc gatccgccac gatcagaggc
     2041 ctggcatccg gtcgatgacc ttgccctcgg catcgaacgt gatgccgacg aatcccccgg
     2101 gcatgaagcg cggcttgccc tcgtcgtcgc ggctgtgaac gaaggagacg atgaataccc
     2161 attggccgtc ggtgaaactg tccttgacca cgcagtcgcg tttcgcatcg cagccggctg
     2221 ccgcaagtgc aatcgcccgc gccttgtcct cggtgatcgg caccgcctcg gcgcgcagcg
     2281 tcaacggcag gcagagcgac accatcagca gcgcacccga cagcacaccc accaggcgac
     2341 gcagccggcg caccgcaagc ccatcgactg cacgctgcaa cagacggctg caatgcattc
     2401 agaacatccc gttgtcgttg ctggcttccc ccggcaccac ctgcaacacg tcccagtgct
     2461 cgacgatctt gccggcttcg tcgaagcgga agatatcgat gccggcccag tcgcggttgg
     2521 cgtcggtcgg ccagacctga tggcaatgca gcacgaccag atcgccctcg gcgacggcgc
     2581 gcttgaacga tacccgcttg cccggatact cgcgggccat gcgctcgaag taatcgacga
     2641 aggccgcctt gccgtcggcc acgtgcgggt tgtgttgcgt gtaggtatct ccggtgtaac
     2701 gctcgatcgc ctcgcgtgga cggcactggt tgaacatcag atcgtagaag gcgatggcgt
     2761 tgcgccggtt ctcttctgga ctggtcatgc ggattccctt cgcgctgaac gcggcggcct
     2821 cgcgctaaac cgccgctgtc gggttgactg ccgccagcgc gatacgatcg cctacccgca
     2881 gcatgccgcc ggcgacgatg cgcgcggtca tgccgccgtg cccgcgcagc gcgttgtagc
     2941 cgccgggacc caactcgtct tccatcttcg agcagggatc gcaggggccg gtgagaacga
     3001 cgcgcaccga atcgccaatc gcccattcga gctcaaagtc gcggaagggc gagcgcatcg
     3061 cgatcaggtt gatgcccgag accaccagat tgcggcgcag gcggcacgga tcgacagcat
     3121 cgatcccgca ccagcgggcc agcacgtcca gatgctcggc ctgaaacagc gtgaggtcgc
     3181 gcttctgcgc agcggcgctc cggcgcaccg tcgttgcgcg acgatcgccg agcaggccgc
     3241 ggcctggctc ggccagcacc tcgctgacct gactagccgc cgcatcgcgc gccgggcgca
     3301 gcacgatcgc ttccagccga ccctcggcgg aaaactgccg ggcaagttcc tgcagcgaca
     3361 cgtcgcgttc tccttgcgct gatgccggcg atctcggccg gcgaggccag attgtgctcc
     3421 ggcagacggg ctcgcgaaag cgcggacgaa tcgacgacgc gacggatcac tccgccttgg
     3481 cgccgcgcac ctgatcgaac atcgctgcca cgcttttccc cgctggcccg cgcaacgcga
     3541 tctcgaccgg cgaatcgcga atgctgacca cgatgcgccg gctcagaaag gcaacccgct
     3601 ccttgaccga cagcacctcc gaccacggca gaaagaacgc cgggcagagc aggcgaaacg
     3661 gctgacagat cgacatcgcg atgccttccg gcgccacggt cactgccagc acaccgccgt
     3721 aactgaccgg aaaccacgac actaggccca tctttgccga ggcgaagcga aagtgctcgc
     3781 cggcggcatc cggccgcgcc ggaaaacgac gtgccagcga gcgccagccc gacagcaccg
     3841 aaaacagcac ggctatgccg acggccgcgg cgataaaggc cacggcaaac caaggcgatt
     3901 gatagaacag ggggagctcg gaatcaggca ttgatgggtt ccacagttcg aaagcgggag
     3961 ccgcgcggtg cagatcatcg cgcggcagcg cggatcaaac agcgtggcag cacttcgcat
     4021 gcaactcaac ggcgaagcgc gtcagtctgt cgccttgctc gcgcttcgac aagcagccca
     4081 gcatcacgac tggcacggtg ctcagccagt gccgtgcagc ggcgaccggc gaaatcccga
     4141 ccgcgacaag gtgatgggga attcggcaag cgactaccac cggaccagtt ctccggcgtc
     4201 gaagaccatt ccggaatgcg cctgccggta gttgatgaag atgttctggc gcggaattcc
     4261 ggtgcgcccg gcgatgctga ccgccaccgc cagcagcgcc gtttcgatct gctccggggt
     4321 ctggaaatca ggcgccagaa tctcgacgag taccggatgc gaagcctcgg gctggcactg
     4381 ggcggtgata ccggcgacgg catagtaccc cggcagcagg acctgccagg tcgcggtgac
     4441 gtgctccagc cccaccccgg tggcttcggc gaagtcattt gagatgcccg caacaacctc
     4501 aggaacgtca aacggctggt cgaaaggcag cgatgtgatg tgaacaaacg gcacgtttcc
     4561 tccttcttgc acccaggctt aacgccaccg gcggcgcacg ggcttgtcgc gctggaatcg
     4621 actacccgag cctcgccgac cactcacagc aagcgacgcc cctgcggcgt atcgatgtgc
     4681 gcgaccaggc gtgcgctgcg cctgtcgccc gatggcaata ccgacagcgg ccccgcgaaa
     4741 tcgatcgagc cgagcaggtg cgagatacgt tccggctcgg gatgaaacaa ctcgagtcgg
     4801 acgagcgaca gcccatgatc tatcagcttc gccgccggat gcggcgccgc ctgccattcg
     4861 atcagcgccg gtgcgatgcc gcccagcggc agcgagccgt cggcgggaat ggtgatgagc
     4921 cagtcgagcg cgccgcggct catcggttcg accgctccca gcacctccga acaggcggca
     4981 acgctcgccc gaatatcggc cgtacgcgcc acccagttgg aaagcaccgg ctcggcgccc
     5041 ggctgcagcg tatcgagcgc aaaccagcgt ggccgtgccg gcgccggcga tgccgggttc
     5101 ggggcgatga cttccagaaa cagcgaatcg ccgagccgca gcagtagatt gtgcgttgcc
     5161 atccgcggat gctcgccgcc ggcctgaggc acaacaccga gcgcctggcg cacgctctcg
     5221 gcgccggctt ccagcgttgg cgccgtgatg gtgatgtggt cgatgcagca gtgcgccatt
     5281 caagccaccc gccgtttcgg cagcaacggc cacacgcacg cccc
//
LOCUS       DATDAA010000051         4858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_213413,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000051 DATDAA010000000
VERSION     DATDAA010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213413"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /gene="mnmD"
                     /locus_tag="VIS73_01745"
     CDS             complement(<1..1303)
                     /gene="mnmD"
                     /locus_tag="VIS73_01745"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HEY5761911.1"
                     /translation="MPITPATLAFDANGTPYSETYGDIYHSARGGLEQARHVFLGGNG
                     LPQRWGGRHQFTILETGFGQGLNFLTTWQAWREDPQRSARLHFLSVEKHPFSRSDLAE
                     LHRRHPEIAELSAQLRGQWPELTAGFHRLEFENGELTLTLAFGDAIACVPQFVAAPDA
                     IFLDGFSPAKNPDLWSAPLLREIAWLAAPGATTATWAVAAPVREHLAQAGFRWEKARG
                     YAAKHEMLVATLDDDAAPIPAGKSAKRANTRIAVVGAGLSGSLTAERLAAKGFTVTLF
                     ERHARPAQETSGNLAAAMLPVLSLDDARLSRLNRASFLYANRYLARFGFGTANSPVRG
                     ASSGVLYIARDAAHAEKQREILARNGFPESFAHWVDAEAGSELVGARVAGGGWWFPNG
                     SYANPASLCSAALTGSAVSLRFNTPVAAVSEIDGTWGLSGAD"
     gene            complement(1445..2950)
                     /gene="lysS"
                     /locus_tag="VIS73_01750"
     CDS             complement(1445..2950)
                     /gene="lysS"
                     /locus_tag="VIS73_01750"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY5761912.1"
                     /translation="MSDQLPEQPQQDENHIIAERREKLARWREEGAAYPNDFRRENIA
                     GKLIELYGEKSREDLEAMPVQVKIAGRMMLKRVMGKAAFATLQDLSGRIQIYVARDSV
                     GDDTLDAFKHWDIGDIVGAVGTVFKTKTGELTVHCEKVRLLSKCIRPLPEKFHGLTDI
                     EQKYRHRHLDLITNDQARYTFLARSRMMQSIRNYMIGHGFLEVETPMMHPIPGGASAK
                     PFVTHHNALDMEMFLRIAPELYLKRLVVGGFEKVFEVNRNFRNEGISPRHNPEFTMME
                     FYEAYADYHMLMDFTEGLLRHAAREALGTELFYYQGKPLDLGKPFARLTISEAVRKHH
                     PKYTEENLRDTAWLRAKLKDLKVPVRDGAGLGTLQMQLFEETTEAELWEPTFIIDYPT
                     EVSPLARKNDANPEVTERFELYIVGREIANGFSELNDPEDQANRFHDQAAAKAEGDEE
                     AMFFDADYIHCLEYGLPPTGGCGIGIDRLVMLLTDSPSIRDVILFPQMRRS"
     gene            complement(3005..4109)
                     /gene="prfB"
                     /locus_tag="VIS73_01755"
     CDS             complement(join(3005..4027,4029..4109))
                     /gene="prfB"
                     /locus_tag="VIS73_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237878.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY5761913.1"
                     /translation="MEAEQLNTLEEQLDDLAARSTDLRRYLDYAGKRDRLTEVVAALE
                     QPEVWNDAKRAQELGRERHALESTVSALESIDSGIRDTRELFDMAREEKDYETIGGLV
                     ADITAIEDKVKQLEFRRMFNNPQDPSPCFLEIQAGAGGTEAQDWASMLLRMYLRYGER
                     REFKIDVLEESEGDVAGIKSATLRLEGDYAYGMLRTETGIHRLVRKSPFDSNARRHTS
                     FASVFVYPEVDDSIEIEISPADVRTDTYRASGAGGQHINKTDSAVRLTHVPTGIVVQC
                     QNDRSQHRNRDEAWKMLRARLYEHELRKQQAEQQKLEDAKSDIGWGHQIRSYVLDQSR
                     IKDLRTNYEVGNTQAVLDGDLDDFIEASLKQGV"
     gene            complement(4218..>4858)
                     /locus_tag="VIS73_01760"
     CDS             complement(4218..>4858)
                     /locus_tag="VIS73_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEY5761914.1"
                     /translation="ITVFQPDWHAGVVGILAARIKDRYHRPAFAFARGDAGEVKGSGR
                     SIAGLHLRDALDLISKREPGLLKRFGGHAMAAGVTLAESALPQFERAFAAVSAELVSP
                     TEFTRTLETDGSLESAYFSLDVAQLIEAEVWGSGFPSPLFEDVFEVEQQRLLQDRHLK
                     LRVRKNEVRIDAIRFHFAENVGPRVRLAYRLQVNDFNGVQNPQLLIDHIEAA"
BASE COUNT          767 a         1463 c         1697 g          931 t
ORIGIN      
        1 cgtcggcgcc gctcagcccc caggtgccgt caatctcgct gacggcggcc accggcgtgt
       61 tgaaccgcag cgataccgcg ctgccggtga gcgcggcgct gcacaggctt gccggattgg
      121 cgtagctgcc gttggggaac caccagcctc cgccggcgac gcgggcgccg accagttcgc
      181 ttcccgcctc ggcgtcgacc cagtgggcaa aggattcggg aaagccgttg cgggcgagga
      241 tctcacgctg cttctcggcg tgcgccgcgt cgcgggcgat gtagagcacg ccgctggacg
      301 cgccgcgcac cggtgagttc gcggtgccga agccgaagcg ggcgaggtag cggttggcgt
      361 agagaaagga cgcgcggttc agccgcgaca accgcgcgtc atcgagtgag agcaccggca
      421 gcatcgctgc agccagattg cccgaggttt cctgggcggg ccgtgcgtgg cgctcgaaca
      481 gcgtgacggt gaaacccttg gcggcaagcc gttcggcagt caacgatccc gagagtccag
      541 cgccgacgac ggcgatgcgg gtattggccc gcttggcgga tttcccggcc gggatcggtg
      601 cggcatcgtc gtccagcgtc gcgaccagca tctcgtgctt ggcggcgtag ccgcgggcct
      661 tctcccagcg gaagcccgct tgcgcgaggt gttcgcgaac cggcgccgcc accgcccagg
      721 ttgccgtggt ggcgccgggt gcagcgagcc aggcgatctc acgcagcagt ggcgccgacc
      781 agagatcggg attcttcgcc ggcgaaaaac catcgagaaa gatcgcgtcg ggcgccgcga
      841 cgaactgcgg aacgcaggca atggcgtcgc cgaaggccag cgtcagcgtc agctccccgt
      901 tctcgaattc caggcggtgg aaaccggcgg tgagttcggg ccactgtccg cgcagttgcg
      961 ccgacagttc ggcgatctcc gggtggcgac ggtgcaattc agcgagatcg ctgcgcgaaa
     1021 aggggtgctt ctcaaccgaa agaaagtgca ggcgcgccga ccgctggggg tcctcacgcc
     1081 aagcctgcca ggtggtgagg aagttcagtc cctggccgaa gccggtttca aggatcgtaa
     1141 actggtggcg tcctccccag cgctgcggca gcccattgcc gccgaggaac acatggcgcg
     1201 cctgttcaag cccgccgcgt gcgctgtggt agatgtcgcc gtaggtttcc gaatacggcg
     1261 tgccgttcgc atcgaaggcg agcgtggccg gcgtgatcgg cattgcctta gccgcggggc
     1321 ttgaatgcgc cggccggatt tgaggatcga gcgagcgcag gcgagacgag gcgcccgtgg
     1381 tcgccgcata gcagcgctat gcaagaccgc gggcaacgac gcatggccga gcgcagcctt
     1441 ttcctcagga gcggcgcatc tgggggaaga ggatcacgtc tcttattgac ggtgaatcgg
     1501 tcagcagcat caccagccgg tcgatgccaa tgccgcaacc gccagtgggc ggcagaccat
     1561 actcgaggca atggatgtag tcggcgtcga agaacatcgc ctcttcgtcg ccttcggcct
     1621 tggccgctgc ctgatcgtgg aagcggttgg cctgatcctc ggggtcgttc agctccgaga
     1681 agccgttggc gatctcgcgg ccgacgatgt agagctcgaa gcgctcggtg acctctggat
     1741 tggcgtcgtt cttgcgcgcc agcggactga cttctgtcgg gtagtcgatg atgaaggtcg
     1801 gctcccagag ttcggcctcg gtggtttcct cgaacagctg catctgcagc gtgcccaggc
     1861 cagcaccatc gcggaccgga accttgaggt ccttgagctt ggcgcgtagc caagcggtat
     1921 ctcgcaggtt ctcttcggtg tacttgggat ggtgcttgcg cacggcctcg ctgatggtca
     1981 gccgcgcgaa gggcttgccg agatcgagcg gcttgccctg gtagtagaag agttcggtgc
     2041 cgagcgcctc gcgcgccgcg tgccgaagca ggccctcggt gaagtccatc agcatgtggt
     2101 agtcggcgta ggcctcgtag aactccatca tggtgaactc ggggttgtgc cgcggcgaga
     2161 tgccctcgtt gcggaagttg cggttcacct cgaagacctt ctcgaagccg ccgacaacga
     2221 ggcgcttgag gtagagctcg ggagcgatgc gcaggaacat ctccatgtcc agcgcgttgt
     2281 ggtgggtgac gaagggcttg gccgaggcgc cgccgggtat cgggtgcatc atcggcgttt
     2341 cgacttcgag gaagccgtgg ccgatcatgt agttgcggat cgactgcatc atccggctgc
     2401 gtgccaggaa ggtgtagcgc gcctggtcgt tggtgatcag gtcgaggtgg cgatgacggt
     2461 acttctgctc gatgtcggtg aggccgtgga atttttcggg cagggggcgg atgcacttgg
     2521 agagtaggcg aaccttttcg caatgaacgg tcagctcgcc ggtcttggtc ttgaacacgg
     2581 tgccgacggc gccgacgatg tcgccgatgt cccagtgctt gaaggcgtcc agcgtgtcgt
     2641 cgccgacgct gtcgcgagcg acgtagatct ggatgcggcc cgagaggtcc tgcagcgttg
     2701 cgaaggcagc cttgcccatg acgcgcttga gcatcatccg gccggcgatc ttcacctgga
     2761 ccggcattgc ttcgaggtct tcgcgcgatt tctcgccgta gagctcgatc agcttgccgg
     2821 cgatgttttc gcggcggaaa tcgttgggat aggccgctcc ttcttcacgc cagcgggcca
     2881 gcttttcgcg gcgctcggcg atgatgtggt tctcgtcctg ttgcggttgt tccggaagtt
     2941 gatcgctcat ggtctgggtc ctgtgggcgt cgtcgccccg ctgcggtaag ggtagtgagg
     3001 gtttctagac gccctgcttg aggctggcct cgatgaagtc gtcgaggtcg ccgtcgagga
     3061 cggcctgggt gttgccgacc tcgtaattgg tgcgcaggtc cttgatccgt gactggtcga
     3121 gcacgtagct gcggatctgg tgtccccagc cgatgtccga cttggcgtct tcgagctttt
     3181 gctgttcggc ctgctgcttg cgcaactcgt gctcgtagag gcgggcgcgc agcatcttcc
     3241 aggcctcgtc gcggttgcgg tgctgcgagc ggtcgttctg gcactggacg acgatgccgg
     3301 tcggcacgtg tgtcagacgc acggcggagt cggtcttgtt gatgtgctgg ccgccggcgc
     3361 ccgaggcgcg ataggtgtcg gtgcgcacat cggccggact aatctcgatt tcgatcgagt
     3421 cgtcgacttc ggggtagacg aaaactgaag caaacgaggt gtggcggcgc gcgttggaat
     3481 cgaagggcga cttgcggacc aggcggtgga tgccggtctc ggtgcgcagc atgccgtagg
     3541 cgtagtcgcc ctcgaggcgc agcgtcgcgc tcttgatgcc ggcgacgtcg ccttcggatt
     3601 cctcgagcac gtcgatcttg aattcgcgcc gttcgccgta gcgcaggtac atccgcagca
     3661 gcatcgaggc ccaatcctgc gcctcggtgc caccggcgcc ggcctggatt tccaggaagc
     3721 aggggctggg gtcctgcgga ttgttgaaca tccgccggaa ttcgagctgc ttgaccttgt
     3781 cctcgatggc ggtgatgtcg gcgacgaggc cgccgatcgt ctcgtagtcc ttctcttcgc
     3841 gcgccatgtc gaatagctcg cgggtgtctc ggattcccga atcgattgat tcgagcgcgc
     3901 tgaccgtcga ttcgagcgca tggcgctcgc ggccgagttc ctgggcgcgc ttggcgtcgt
     3961 tccagacttc gggttgctcg agcgcagcga cgacttccgt caggcgatcg cgtttgccag
     4021 cgtagtcaaa gatacctccg gagatctgtt gaacgagccg ccaggtcgtc caactgttct
     4081 tcgagtgtat tgagttgttc tgcttccatg atgtccggtc gttcgccggt cggcgtgatg
     4141 ctgccggcaa tttccaaagc ggcggattat acctgctgca ccgcaagaac ggccttccgc
     4201 gcgcgccggg aaagccttca tgcagcctcg atgtggtcga tcagcagctg cggattctgc
     4261 acgccgttga agtcgttgac ctgcaggcgg taggccaggc gcacccgcgg accgacgttt
     4321 tcggcgaaat ggaagcggat ggcgtcgatg cgaacctcgt tcttgcgcac ccgcagcttc
     4381 aggtgccgat cctgcaagag ccgttgctgc tcgacttcga agacatcctc gaacagcggc
     4441 gagggaaagc ccgagcccca gacttcggcc tcgatcagtt gcgccacgtc gagcgagaag
     4501 tacgccgatt cgagcgatcc atccgtctcg agcgtgcgcg tgaactcggt tggcgacacc
     4561 agttcggccg ataccgcggc aaaggcgcgc tcgaactgcg gcagcgcgct ctcggcgagc
     4621 gtcacgccgg cagccatcgc gtggccgccg aagcgcttga gcaggccagg ctcgcgcttg
     4681 gagatcaggt cgagcgcgtc gcgcaggtgc aggccggcaa tcgaccggcc ggagcccttg
     4741 acctcgccgg cgtcgccgcg ggcgaaggcg aaggcgggcc ggtgatagcg gtccttgatc
     4801 cgcgccgcga ggatgccgac gacgcccgcg tgccagtcgg gctggaagac cgtaattg
//
LOCUS       DATDAA010000052         9560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_283322,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000052 DATDAA010000000
VERSION     DATDAA010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9560
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283322"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..7294)
                     /locus_tag="VIS73_01765"
     CDS             complement(<1..7294)
                     /locus_tag="VIS73_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY5761915.1"
                     /translation="MSLAWRKGKASLKKRWLTRRERITAKRLGQGAAEGPLRAELFST
                     EQMAQHGVRLAAEHVLAPGRIADRLLARLAANEALLIESCRQLQAAIAANHRITPAGE
                     WLLDNFHLIEEQIRTARRHLPKGYSRELPGLASGPSAGLPRVYDIALKTIAHGDGRVD
                     PENLGRFVSAYQSVTPLRLGELWAIPIMLRLALIENLRRVATQISAGRTDRNLAVAWA
                     DKLTDIAHRDPKNLILVIADMARSSPPMSSPFVSELARRLQGQGPALALPLTWIEQRL
                     TESGLDIERLVRAGNQSQAADQVSVSNSIGSLRCLGTMDWRDFVESMSLVEQTLRADP
                     VAAYARMDFASRDRYRHVIEAVAKQSEFSELAVAGNAVELAQAGKAKAGAGDRIAHVG
                     YYLIDRGLDDLAKATRMRRSPAQAVQRWAARFPLTLYLGAIAVISLVASGYLLSEAWA
                     TGSAASWSAWVLLPLAGAALLANSQFAVSLVNSLVSVLASPHPLPRMDFSSGIPSSAR
                     TLVVVPTMLTSPRNVEALVEALEVRFLANRDEHLHFALLTDLCDAHAEVLADDAPLLL
                     LAREQIEALNARYGCGRNDRFFLLHRPRRWNPHDRIWMGYERKRGKLADLNALLRPPA
                     NREGGGGFALVVGTTEVLADVQYVITLDTDTQLPRDAARQFVGAMAHPLNRPVYDEAL
                     GRVSAGYGILQPRVSVSLPGTGQSRYTRLHGGEPGIDPYTRVVSDVYQDAFEEGSFIG
                     KGIYDVDAFEQSLKGRFPENRILSHDLLEGCYARAGLLSDVQLYEEYPSRYGADVNRR
                     YRWIRGDWQLAGWLLPWVPGPAGWQRHNPLSALSRWKLFDNLRRSLAPAVLTLLLLLG
                     WTVLAAPLFWTLAVLGILLIPPLLATVLDVMRKPDDMRPGQHLAAATHAAGQRFLQLG
                     FTVTTLPHEAFYSLDAILRTLGRMLLTRQRLLEWNPSGDQERLSRSDLLASFRAMWFA
                     PTLAIPGAIVVAQANPAALYLAGPFLILWLASPLIAWWVSLPVARREPRLTAEQTRFL
                     GRIARKTWAFFDRFVGPEDHWLPPDNYQEYRAAAVAHRTSPTNMGLALLANLSAYDFG
                     YIPAGRLVERTGHALATMAGLARHRGHFYNWYDTQTLQVLRPAYVSTVDSGNLAGHLL
                     TLRQGLLALPDRPILEDRWLDGLLDTLGILEEALGDIIPATLTPFRQALEAGLASRPA
                     TLTAARRLLDELNRHAAALCATLGSETAGEASWWASALAAQCRTARDALAFLAPGHGP
                     HEAPGREPDAIPTLRSLAADSARARERIAAIERLATQAGVLAEMEYGFLYDKAARLLC
                     IGYNVDERRLDTGYYDLLASEVRLSVFVAIAQGRLPQESWFALGRLLTSAGGKPVLLS
                     WSGSMFEYLMPGLVMPSYEHTLLDQTAQAAVARQIDYGQQAGLPWGMSESGYNTVDAE
                     LNYQYRAFGVPGLGLKRGLAEDMVVAPYASALALMVAPEAACANLRRLADAGAEGQFG
                     FHEAIDYTPARQRRDESHALIRSYMAHHQGMSLLALAHLLLGQPMQKRFVADPLFQST
                     LLLLQERVPKAASFYAHGAGLDGVHAGTVSPQMPLRVLGNPDTPMPEVQLLSNGRYHV
                     MLTHSGGGYSRWNELALTRWREDGTRDSWGTFAYLRDVASGTFWSTAHQPSRRRAERY
                     EAIFSEGRAEFRRRDSGEAQGDAFDFDTHTDIVVSPEDDIELRRMRITNRSPASRSIE
                     VTSYTEVVLATAAADALHPAFSNLFVQTEIVAAQQAILCTRRPRSRDEPVPWMFHLTA
                     VHDADVDAISYETDRARFIGRGRSAAAPAALDADSTQLSGSQGSVLDPVAAIRCQISL
                     EPGQSVTIDQVYGIGTSRDAALGMVEKYRDRRLADRVFELAWTHAQVLLRQINAREVD
                     AQLYGRLANSILYTNPALRADSSVLARNQRGQSGLWGYAISGDLPIVLLRIGDLANIE
                     LVRQLIQAHGYWRLKGLKVDLVIWNEDRAGYRQALQEQILALVAVGLEPQAIDRPGGI
                     FIRLVEQISAEDRSLFQTVARVILSDSRGTLAEQLARPAAPTPRVPLLAPVRTAPAEP
                     PTAFTLPARELILANGIGGFTPDGHEYVITTTREQRTPAPWANVLANPDFGSVISESG
                     QAYTWGENAHEFRLTPWHNDPIVDASGEAFYLRDEDSGQFWSPTPLPRRGATPYVSRH
                     GFGYSVFEHTESGIVSELWVYVALDAAVKFSVLKVRNASGRPRRLSATGYVEWVLGDL
                     RPKTAMHVVCEVDPDGAALYARNPYNTEFPDRVAFFDADGEGPLERSLTCDRIEFIGR
                     NGSLENPAALLRTHLSGKAGSGLDPCAAIQLRFDLADGAEREICFRLGLGGMPGGDDA
                     QRLVQGHRGVDTARRALEAVWQYWNRTLGAVQVETPDQSLNVLANGWLLYQTIACRMW
                     ARSGSYQSG"
     gene            8775..9122
                     /locus_tag="VIS73_01770"
     CDS             8775..9122
                     /locus_tag="VIS73_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761916.1"
                     /translation="MTNVVHHYPHVIPDSEKQHAQDLGISLNGTQFGYREFKYDKLPD
                     AITYAELDALRQGRQKDVSHAPEWLDQTAPCASDQELMRQYEITSEGWRYRYREYRYD
                     RLADAINYAANQN"
     gene            9224..9544
                     /locus_tag="VIS73_01775"
     CDS             9224..9544
                     /locus_tag="VIS73_01775"
                     /inference="COORDINATES: protein motif:HMM:NF020469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 family protein"
                     /protein_id="HEY5761917.1"
                     /translation="MTITFKSEGRASVEMKQKNAHELLKILGKESTDGRGVFAPDQLG
                     SAISVLKRLISDNADRRRDLANAEIDEKAGEVFEVDISLRAMPLLELFEDAQKSQKPV
                     TWGF"
BASE COUNT         1683 a         2972 c         3272 g         1633 t
ORIGIN      
        1 cgcccgactg gtaagagccg ctgcgcgccc acatccggca ggctatcgtc tggtagagca
       61 gccagccgtt ggcgagcacg ttgagtgatt ggtccggcgt ttcgacctga acggcgccga
      121 gggtccggtt ccagtattgc cagaccgcct cgagggcgcg ccgcgccgta tcgacgccgc
      181 gatggccttg caccaggcgc tgggcgtcgt cgccgcccgg catgccgccg agtcccaacc
      241 ggaagcagat ctcgcgctcg gcaccgtcgg ccagatcgaa gcgcaactgg atggccgcgc
      301 agggatcgag gccgctgccc gccttgccgc tcaggtgggt gcgcagcagc gccgccgggt
      361 tctcaaggct accgttgcga ccgatgaact cgatccggtc gcaggtgagg ctgcgctcca
      421 gcgggccctc gccgtcggcg tcgaagaagg cgacccggtc gggaaactcg gtgttgtacg
      481 gatttcgcgc gtagagcgcg gcgccatccg gatcgacttc gcagacgaca tgcatggcgg
      541 ttttcgggcg caggtcgccg agcacccatt ccacatagcc ggtcgcggac agtcggcgcg
      601 gccggcccga ggcgttgcgt accttgagca cggagaattt gactgcggca tcgagcgcca
      661 catagaccca cagctcggag acgattcccg actcggtgtg ctcgaacacg ctgtagccga
      721 agccatggcg gctgacgtag ggcgtcgcgc cgcgccgggg cagcggcgtc ggcgaccaga
      781 actggccgct atcctcgtcg cgcaggtaga aggcctcgcc gctcgcatcg acgatcgggt
      841 cgttgtgcca gggggtgagg cggaattcgt gggcgttctc gccccaggtg taggcctgac
      901 cgctttccga aatgacgctg ccgaaatcag gattggccag tacgttcgcc cacggcgccg
      961 gcgtgcgctg ctcgcgggtg gttgtgatga cgtactcatg accatccggg gtgaagccgc
     1021 cgattccgtt ggcaaggatc aactcgcgag cgggcagagt gaaggcggtc gggggttccg
     1081 cgggcgccgt cctgacgggc gccagcagcg gtacgcgcgg cgtcggcgcg gcgggacggg
     1141 cgagttgttc tgccagggtt ccccggctgt cgctgaggat gactcgggca acggtctgga
     1201 acaggctgcg gtcctcggcc gagatctgtt ccaccaagcg aatgaagatg ccgcccggcc
     1261 ggtcgatcgc ctgcggttcg agacccaccg ctaccagcgc gagaatctgc tcctgaaggg
     1321 cctgccggta gccggcgcgg tcttcgttcc agatcactag gtccaccttc agccccttca
     1381 gccgccagta gccatgggcc tgaatgagct ggcgcaccag ttcgatgttg gcgaggtcgc
     1441 cgatgcgcag caggacgatc ggcaggtcgc cggaaatggc gtagccccag aggccggatt
     1501 gaccgcgctg gttccgcgcg agcacgctgc tgtcggcgcg tagcgcggga ttggtgtaga
     1561 ggatcgagtt ggccaggcgg ccgtagagct gcgcgtctac ctcgcgggcg tttatttggc
     1621 gcagcaagac ctgcgcatgg gtccacgcca gttcgaagac gcggtcggcg aggcgccggt
     1681 cacgatattt ttcgaccatg ccgagggctg cgtcgcggct ggtgccgatc ccatagacct
     1741 gatcgatggt gaccgactgc cccggttcga ggctgatctg gcaacggatg gccgccaccg
     1801 gatcgagcac cgatccttgg ctgccggaaa gctgcgtcga gtcggcatcg agcgccgccg
     1861 gcgcggcggc gctgcggccg cggccgatga agcgcgcgcg gtcggtttcg taggaaatgg
     1921 cgtcgacgtc ggcgtcgtgc acggccgtca gatgaaacat ccagggcacc ggctcgtcgc
     1981 gcgagcgggg acggcgggtg cacaagatcg cctgttgcgc cgcgacgatt tcggtctgca
     2041 cgaaaagatt gctgaacgcc gggtgcagcg cgtcggcggc ggccgtggcc agcacgacct
     2101 cggtgtagct cgtgacctct atgctgcggc tggcggggga tcggttggtt atgcgcatcc
     2161 gccgcagttc gatgtcgtct tccggagaga cgacgatatc ggtgtgggta tcgaaatcga
     2221 aagcgtcacc ctgtgcttcc ccgctgtcgc ggcggcggaa ttcggcacgc ccttccgaga
     2281 agatcgcttc atagcgttcg gcgcgccgcc ggctgggctg gtgcgccgtg gaccagaacg
     2341 tgccgctggc gacgtcgcgc aggtaggcga aggtgcccca ggaatcgcgg gtgccgtctt
     2401 cgcgccagcg tgtcagcgcc agctcgttcc agcggctgta gccgccgccg gagtgggtga
     2461 gcatgacgtg gtagcggccg ttggacagca gttgcacctc cggcatcggc gtgtccggat
     2521 tgcccagcac gcgcagcggc atctgcgggc tgaccgtgcc ggcatggaca ccgtcgagcc
     2581 cggcgccgtg ggcatagaag gaggcggcct tggggacgcg ttcctgcaag agcagcaggg
     2641 tggactgaaa cagcgggtcg gcgacgaagc gcttctgcat tggctgcccc agcagcaggt
     2701 gcgccaaggc cagcaggctc atcccctggt gatgggccat gtacgagcgg atcagggcat
     2761 gggattcgtc gcgccgttgc cgtgccggcg tgtaatcgat ggcctcatga aaaccaaact
     2821 gcccttcggc accggcgtcg gcaagtcgcc tcaggttcgc gcaggccgct tccggtgcaa
     2881 ccatcagcgc cagcgccgaa gcgtagggcg cgacgaccat gtcctcggcc aggccgcgtt
     2941 tcaggcccag accgggtacg ccgaaggcgc ggtactgata gttgagctcg gcgtccaccg
     3001 tgttgtagcc ggattctgac atcccccacg gcaagcccgc ttgctggccg taatcgatct
     3061 gccttgccac cgccgcctgg gccgtctggt caaggagggt gtgctcgtag ctgggcatca
     3121 ccaggcccgg catcaggtac tcgaacatcg agccgctcca cgagagcaga accggcttgc
     3181 cgccggcgct ggtcagcagc cgtccgagcg cgaaccagct ttcctggggc agccgcccct
     3241 gggcaatggc gacgaacacg gacaagcgca cttccgaggc cagcaggtcg taatagccgg
     3301 tatccagccg ccgctcgtcg acgttgtagc cgatgcacaa caggcgcgcc gccttgtcgt
     3361 agaggaagcc gtattccatc tcggcgagta cgccggcctg cgtggcaagc cgctcgatgg
     3421 ccgcaatgcg ttcgcgggcg cgcgcactgt ccgctgccag ggaccggagc gtcgggatgg
     3481 cgtccggttc cctgccgggc gcctcatgcg gaccatgccc cggcgcgaga aaggccagcg
     3541 catcgcgggc cgttcggcat tgcgccgcga gcgcgctggc ccaccagctc gcttcgccgg
     3601 cggtctcgct acccagcgtc gcgcagagcg cggcggcgtg ccggttcaac tcgtcgagca
     3661 gacgccgtgc tgcggtcagc gttgccggcc gggaagcgag gccggcctca agcgcctgcc
     3721 ggaatggtgt cagggtggcg ggaatgatgt cgccgagcgc ttcctcaagg atgcccagcg
     3781 tgtcgagcag cccgtcgagc caccggtctt cgaggatcgg ccggtcgggc agcgcgagca
     3841 gtccttggcg cagggtcagt agatggccgg ccaagttgcc gctgtccact gtcgacacgt
     3901 acgcggggcg caacacctgc agggtctgcg tgtcgtacca attgtagaaa tgcccccggt
     3961 ggcgggcgag tccggccatc gtcgccagag catgcccggt gcgctcgacc agacgcccgg
     4021 cggggatgta gccgaagtca taggcagaca gattcgccag cagtgccagc cccatgttgg
     4081 tcggcgaggt gcgatgcgcc accgcggcgg cgcggtattc ctgatagttg tccgggggca
     4141 gccagtggtc ttcggggccg acaaagcggt cgaagaaggc ccaggtcttg cgcgcgatgc
     4201 ggccgagaaa acgggtctgt tcggccgtca gccggggctc gcgacgagcg acgggcaggc
     4261 tgacccacca ggcaatgagg ggcgatgcta gccacaggat caggaagggc ccagccaggt
     4321 acagcgcggc gggattggcc tgcgcgacga cgatcgcgcc gggaatcgcc agcgtcggcg
     4381 caaaccacat ggcgcggaac gaggcaagca ggtcgctgcg gctcagtcgc tcctgatcgc
     4441 ccgatgggtt ccattcgagc agccgctgcc gggtcaacag catgcgaccg agcgtgcgca
     4501 ggatcgcgtc gaggctgtaa aaagcctcgt gcggcagcgt cgtcacggta aagccaagct
     4561 gcaggaagcg ctggccggcg gcgtgggtgg cggccgcgag atgttgcccg ggccgcatgt
     4621 cgtccggctt gcgcatcaca tcgagcaccg tcgccagcag cggcggaatc agcaggatgc
     4681 cgagcaccgc cagagtccag aagagtggag cggccagcac cgtccagccg agcagcagga
     4741 gcagcgttag taccgccggc gccaggctgc ggcgcaggtt gtcgaacagc ttccagcgcg
     4801 acagcgccga gagcgggttg tggcgctgcc acccggctgg gcccggtacc cagggcagca
     4861 gccagccggc gagttgccaa tcgccgcgga tccagcggta gcggcgattg acgtcggcgc
     4921 cgtagcggga cgggtattcc tcgtacagct gtacgtcgct taacagcccg gcgcgggcgt
     4981 aacagccttc cagcagatcg tggctgagga tgcggttttc gggaaaacgc cccttgagcg
     5041 attgctcgaa ggcatcgacg tcgtagatcc ccttaccgat gaaggagcct tcctcgaacg
     5101 cgtcctggta cacgtcggag accacgcgcg tgtacgggtc gatgcccggt tcgccgccat
     5161 gcaggcgggt atagcgcgac tggccggtgc cgggcaggct gacgctgacg cgcggttgca
     5221 ggatgccgta gccggcgctg acccgcccca gcgcttcgtc atagaccggg cgattgagcg
     5281 ggtgggccat ggcgccgacg aactggcgcg cggcgtcgcg cggcagttgc gtgtcggtgt
     5341 ccagggtaat cacgtattgg acgtcggcga gcacctcggt ggtgccgacc accagcgcga
     5401 agcctccgcc gccttcgcga ttcgcaggcg gtcgaagcag ggcgttgagg tcggccagct
     5461 tgccgcgctt gcgctcgtag cccatccaga tccggtcgtg ggggttccag cggcgcggcc
     5521 gatgcaggag gaagaagcgg tcgttgcggc cgcatccgta gcgcgcgttc agcgcttcga
     5581 tctgttcacg ggccaggagc agcaggggcg catcatcggc cagtacttcc gcgtgcgcgt
     5641 cgcacaggtc ggtcagcagc gcgaagtgaa gatgctcgtc gcggttggcg aggaacctca
     5701 cttccagcgc ttcgaccagc gcctcgacgt tgcgcgggct ggtgagcatg gtcggcacca
     5761 ccaccaatgt acgggccgac gacgggatgc ccgaggagaa atccatgcgc ggcagtggat
     5821 ggggcgacgc cagcaccgag accagtgaat tcaccaatga aacggcgaac tggctgttgg
     5881 ccagcagcgc agcgcctgcc agcggcaaca gcacccatgc gctccagctg gctgccgatc
     5941 cggttgccca cgcctcgctc agcaggtagc cgctggcgac cagcgagatc accgcaatcg
     6001 cacccagata gagcgtcaag gggaagcgcg ccgcccagcg ctgaactgcc tgcgccggcg
     6061 aacgacgcat ccgggttgcc ttggccaggt cgtcgagccc ccggtctatc aggtaatagc
     6121 cgacgtgggc gatccggtcg ccggctccgg ccttggcctt cccggcctgc gccagttcga
     6181 ccgcgttgcc ggccaccgcc agttcggaga actcgctctg cttcgccacc gcctcgatga
     6241 catggcgata acggtcgcgg ctggcgaagt ccattcgggc gtaggccgcg acgggatcgg
     6301 cgcgcagcgt ctgttcgact aggctcatgg actcgacgaa atcgcgccag tccatcgttc
     6361 ccaggcagcg cagactgccg atgctgttgc tgaccgagac ttggtcggcg gcctgcgact
     6421 ggtttcccgc gcgcaccagg cgctcgatgt ccagccccga ctcggtgaga cgctgctcga
     6481 tccaggtcaa tggcagcgcc agggcggggc cctgaccctg cagccggcgc gcgagttcgg
     6541 aaacgaaggg cgaactcatc ggcggactcg aacgcgccat gtcggcgatc accaggatca
     6601 ggttcttcgg atcgcgatga gcgatatcgg tcagcttgtc ggcccaggcg acggccaggt
     6661 tccggtcggt cctgccggcg gagatttgcg tcgcgacgcg gcgcaggttt tcgatcagcg
     6721 ccaggcggag catgatcgga atcgcccaca gctcgcctag gcggagcggc gtgaccgact
     6781 gataggcgga gacgaaacgg ccgaggtttt cggggtcgac ccgcccgtcg ccgtgggcga
     6841 tggtcttcag ggcgatgtcg tagacccgtg gcaggccggc cgagggcccg ctagccagac
     6901 ccggcagctc gcggctatag cctttgggca gatggcgccg ggccgttcgg atctgctctt
     6961 cgatgaggtg gaagttgtcg agcagccact ccccggcggg cgtaatgcgg tgattggccg
     7021 cgatcgccgc ctgcaactgc cggcagctct cgatcagcag cgcttcattg gccgccagcc
     7081 gcgccagcag gcgatcggca atgcgtccgg gcgcgaggac atgctcggca gcgaggcgga
     7141 cgccgtgctg ggccatctgt tcggtgctga acaattcggc gcgcagcggc ccttcggctg
     7201 cgccttgccc aaggcgtttt gccgtgatcc gctcgcgacg ggtcagccaa cgcttcttga
     7261 gacttgcctt gccttttcgc cacgccaagc tcatgcaatc ctctccgctg tccagcgaat
     7321 caaagccgtc gcgccgcatg gcgaaattcg gtggcgacga tgcctgggtt gccgtgggcg
     7381 acccccggcg ttagggaatc cttcgcggaa cagcggcgag gggaatggcg cggcctggtt
     7441 gccgtgacac gcgatggcgg tgaggtccga gtcggcgcgc cgggcagaac gccgccgccg
     7501 acgtcgcggc gctgtagcgg cgatgaagcc agctgtgttg gccgcgcaat gaggcgtttc
     7561 gtgcatcgcc gcgcaggcgg tagggccagc caataccgga agagcccgcg tccttccttc
     7621 gccatgctgg tgtgaaactc acgtccggag cgtgggttgc accgtcgcga tcaaacccgc
     7681 aggtttccgc tctgccatcg gcaacggccc cgatctggtt tgtccggcaa cggaaccagc
     7741 atgcgccgtt taccgggaat cgtccgcata actatttaat taagtagctt gcattttgaa
     7801 gatcccgagc cggccggccg atccgatgaa tgatggactt ccggccgtca tcgtcccaga
     7861 tcagggcttc ggcggcaccc cgctgttgcc atcggtacgg aatcggccac cccctcggcc
     7921 gcgaaaccgc ccgcgtaacg acgtacgtgg agttgcactg gccccggcga gggcaagaga
     7981 ttcagacagg cccttcggag gctgtttttt tcgggtatcc agatttcacc gttcatgcca
     8041 gtgacgaata actcggcgca cggttgcttg ccatgaccga cagagttcgg ccgattctgt
     8101 tgaaaaactc atggtgttgg gtaagtggga aaacgcgacc ttcaaagtcg cgctagacgc
     8161 agcgcaagca tgcaagggaa gggtgaagcc actcctgaaa tctcgctgag gagtttttca
     8221 acagaatcgg tcaggagcga ccggtcgaac tatctccgaa ggcatccctc aagggcctcg
     8281 tcatcgctaa gagcagcctg acgacgaagg cgaactgatc ggtctttgtt gcccgtcgta
     8341 cgggccggct cgaaggacag ctatcgccac gcttcttgac agtcacgacg cctcgtctag
     8401 tttttcttgg gcaattcaac gagaacgtcg cgcaagccgc tggcgtctgt cgaaaaactg
     8461 aggctcgatg tggagaaggc tagcgtcctg aggtttttcg acgctcgagc ggaagcagcg
     8521 atcttcggcg aaatgacccg gtgagacacg ctgcgcttaa cttgatcgga gcacgcggta
     8581 gttattggct cgctccacag caacgccgtt tgagtcactt gagatttatc cgatagatgc
     8641 ccgctcgcgt gctacattga aacggcagtt agtcaacggt tcaccttgaa ccttcagttc
     8701 cctcttgggc gaacggcgct tccgttgttg atcagtttgt cgaacggtgt tcgacatcaa
     8761 cggaggcgct ggtcatgaca aacgtcgtcc atcattaccc ccatgtgatt cccgattcag
     8821 aaaagcagca tgcgcaagat ctcgggatct ctttaaacgg aacccagttc ggttatcggg
     8881 aattcaagta cgacaagctg ccggatgcca tcacctatgc cgagttggat gccctccggc
     8941 aggggcggca gaaggatgtc tctcatgctc ccgaatggct tgaccagact gctccttgcg
     9001 catccgatca agagctgatg aggcaatacg aaatcacctc cgaggggtgg cgctatcgtt
     9061 atcgggaata ccgatacgac cggttggccg acgcgataaa ttacgccgca aatcaaaact
     9121 gagccgtcag gcaatcttcg cgtaacggat gcggtttggc gcgctattcg agcgttgcac
     9181 gagccaaacc aacgtcagaa agtaaccagg aaggcgatca aacatgacaa ttacgttcaa
     9241 atcggaaggt cgtgcgagcg ttgaaatgaa acagaagaac gcccatgaac tactcaaaat
     9301 actcggcaag gagtccaccg atggaagggg cgtcttcgcg cccgaccaac taggaagcgc
     9361 gattagcgtg ttgaagcgcc tgatcagcga caacgcggac cgtcgccgcg atttggctaa
     9421 tgcagagatc gatgaaaagg cgggggaggt ctttgaggtc gatatttcgc tgagggcgat
     9481 gccgctgctg gaactgtttg aagacgcgca gaagagtcag aaacctgtga cttgggggtt
     9541 ctagcgggct agatggattg
//
LOCUS       DATDAA010000053        15963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_388242,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000053 DATDAA010000000
VERSION     DATDAA010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 15963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15963
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388242"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VIS73_01780"
     CDS             <1..479
                     /locus_tag="VIS73_01780"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY5761918.1"
                     /translation="FKKDGSVTAGNASGINDAAAFLVLADAAKASAAGYKPMARLVAY
                     GIGGVPNEIMGEGPIPSSKIALAKAGLKLDQMDVIESNEAFAAQSLAVIKGLGIDPAK
                     TNVNGGAIAIGHPVGATGAVIVTKLLHEMQRTKAKYGMATMCIGGGQGITTIYERL"
     gene            complement(528..1427)
                     /gene="rsgA"
                     /locus_tag="VIS73_01785"
     CDS             complement(528..1427)
                     /gene="rsgA"
                     /locus_tag="VIS73_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY5761919.1"
                     /translation="MIRPINAGHSGEYVGRVISAHGRQYRVLTDDGESINCFPRGKKS
                     ELAVGDRVRIQRQSEDQAVVVACLPRTSLLYRSDPYRQKLIAANATQIVIVVATTPGF
                     SIDLVSRCLIAAESQSLKALVVLNKCDLREHIDPARSLLQPLLKIGTRILEISAKCDA
                     SALLPLLAGEASVLVGQSGMGKSTLVNALVPGAMAATREISVALGSGRHTTTHATYYT
                     LDADTALIDSPGLQEFGLAHLDRQTLSESFVEFRPHLGNCRFRDCCHRHEPNCALLEA
                     KARGEIDPVRMASYYRLCDELPK"
     gene            complement(1424..2686)
                     /locus_tag="VIS73_01790"
     CDS             complement(1424..2686)
                     /locus_tag="VIS73_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY5761920.1"
                     /translation="MGTYPSLETLTATTFAAFWLLALTLRLWLANRQITKLQGSRDQP
                     PAVFAAEVSAQENAKAVDYGVDKARFSRWGTVSDAALVMALTFGGGLDLLSGLSAALT
                     SSAIAGGIILLVLLALVNAIVDLPFAWHRQFVIEARHGFNRMTPALFLADLGKAALLS
                     ALLGIPLAWAALQLMASTGANWWLWVWALWSGFNLLLLWLYPAFIAPLFNSFRPLDDA
                     SLAARIEALLTRCGFRAGGLFVMDGSKRSSHGNAYFTGVGNKRRIVFFDTLLEKLSPA
                     QVEAVLAHELGHFHHRHVLKRVALAILGAGLVLALMATLRESPWFFSGLGVNRVSDAA
                     ALIAFSLFFPLLLFPLQPLFNGLSRRDEFEADRYAVNQTSADSLQSALIQLYRDNAAT
                     LDPDRYHSRFFDSHPPAMERIARIRAAT"
     gene            2726..3295
                     /gene="orn"
                     /locus_tag="VIS73_01795"
     CDS             2726..3295
                     /gene="orn"
                     /locus_tag="VIS73_01795"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEY5761921.1"
                     /translation="MAQDASRLVWLDMEMTGLQPDSDRIIELAIVVTDESLVTVAESP
                     VWVVHQSDVVLDGMDSWNKGAHGRSGLIEKVRASTLDEAAVQTAALAFLKEQVPKRAS
                     PMCGNSICQDRRFMARYMPELEAWFHYRNLDVSTIKELCKRWAPALASGFSKHNRHEA
                     LADIYESIEELRYYRGCFLKVASDEPQSA"
     gene            complement(3292..4917)
                     /locus_tag="VIS73_01800"
     CDS             complement(3292..4917)
                     /locus_tag="VIS73_01800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY5761922.1"
                     /translation="MHDTRQRRYAPPPLTRGIPLVVLLALFLASGILGHDPWKSEDAI
                     HFGTIWRAALDSEWLLFSAAEATGPAPPLYFWVARVCGVLFGSSLGLPDAFRIASLLF
                     AGAACALIYLAATELFGKEAAPAAPLALAGCLGFLVQSHETQPLLAVVAAICALLAGV
                     AALHNRQRYATPMLAAGLAGLVLADGLTLLPAGLLILASPLVAAERSQRLATGVRILI
                     ALLCAAVVALPWYLALARAGSTQLAVFLGAELAQLAGVSDFPRNAFRYLNQLGWFAWP
                     AWPLAVWGLWSRRHAWRTSAIWLGAISFFVLWIAIAWIESARAAPALLLLPPLALLAA
                     QGVPQLRRGAAHAFDWFGRLTFGLLAFLLWFGWFAMQFQLPPKVSHNILRLTPGFEPQ
                     TGWLAAGFALTITGLWLWFVVRERRSPLRSIGHWTAGITLVWCLAMSLWLPWIEHLKS
                     YRPVGTELLAALGPAHSGKSCIEPRAVAPAQLSSIEFAMNRRLNPEARPDCRYLLIQG
                     SLDEFPPEPEWRKVWEGNRPGDRHERLRLYHRG"
     gene            complement(4984..5199)
                     /gene="rpmE"
                     /locus_tag="VIS73_01805"
     CDS             complement(4984..5199)
                     /gene="rpmE"
                     /locus_tag="VIS73_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEY5761923.1"
                     /translation="MKAEIHPNYKDVQVTCSCGVAFTTRSTLGKPLQVEVCSECHPFY
                     TGKQKIVDTAGRVEKFNQKFGSLRKPS"
     gene            complement(5322..6578)
                     /gene="rho"
                     /locus_tag="VIS73_01810"
     CDS             complement(5322..6578)
                     /gene="rho"
                     /locus_tag="VIS73_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY5761924.1"
                     /translation="MQLSEIKNLHVSQLLEMALTSGIEGANRLRKQELLFAILKDRAK
                     KGESIFGEGTLEVLPDGFGFLRSPDTSYLAGTDDIYVSPSQIRRFNLHSGDTIEGEIR
                     TPKEGERYVAMVKLDKINGESPEATKNKILFENLTPLHPTRHLRLERDIRSEENTTSR
                     IIDIIAPIGAGQRGLLVASPKSGKTVMLQHIAHAITTNHPDAALIVLLIDERPEEVTE
                     MSRTVRGEVVASTFDEPASRHVGVAEMVIEKAKRLVEHKRDVIILLDSITRLARAYNT
                     VQPASGKVLTGGVDANALQKPKRFFGAARNVEEGGSLTIIATALIDTGSRMDDVIYEE
                     FKGTGNMEIHLDRRMAEKRVYPAINVNRSGTRREELLLEPAVLQKMWLLRKLLYNMDD
                     LEAMEFLLDKIKATKNNAEFFDAMRR"
     gene            complement(6776..7102)
                     /gene="trxA"
                     /locus_tag="VIS73_01815"
     CDS             complement(6776..7102)
                     /gene="trxA"
                     /locus_tag="VIS73_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEY5761925.1"
                     /translation="MSEHIHYVTDDSFDTEVIKSAQPVLVDYWAEWCGPCKMIAPILD
                     EIASEYSGRLKVAKLNIDDNQNVPAKYGIRGIPTLMLFKNGNVEATKVGALSKSALAA
                     FIDSNL"
     gene            7312..7635
                     /gene="fdxA"
                     /locus_tag="VIS73_01820"
     CDS             7312..7635
                     /gene="fdxA"
                     /locus_tag="VIS73_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEY5761926.1"
                     /translation="MTYVVTENCIKCKYTDCVEVCPVDCFHEGPNFLVIDPEECIDCT
                     LCVAECPAEAIFADADVPESQRSFIDLNAELAKSWPVISARKEPAEDADAWLGVPGKL
                     DQLER"
     gene            complement(7632..8771)
                     /locus_tag="VIS73_01825"
     CDS             complement(7632..8771)
                     /locus_tag="VIS73_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5761927.1"
                     /translation="MPGLLPNVDPDGHLEFSVVYTDRALNHMSKRFQGVMTELSRILK
                     TTYGAQACAVVPGGGTYGMEAIARQFATGRRCLVIRNGWFSYRWTQILDAGKIPSATT
                     VLKARQTSPEPTAPFAPVAVEAVVATILAEKPDLVFAPHVETASGILLPDDYLKQIAQ
                     AVHSYGGLFVLDCIASGAVWVDMKGCGVDILVSAPQKGWSGSPGAAFVMLGETARKAI
                     DATTSSSFACDLKKWLQIMETYENGGHAYHATMPTDALKLSCAAMLETEQMGLAAARD
                     AQEALGRAVRSLLAQRGYPSVAAAGFEAPGVVVSYTSDPEIQSGRKFLGLGLQTAAGV
                     PLMCDEGESFRSFRIGLFGLDKLRDVPATVARLADALDRIRGKAS"
     gene            complement(8926..9639)
                     /locus_tag="VIS73_01830"
     CDS             complement(8926..9639)
                     /locus_tag="VIS73_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761928.1"
                     /translation="MRRHLIAAALSLGMTSAALAEDINDVGSLAQSQFRLFSEDIGAT
                     LSYHGQIPAEPLGITGFDIGIAVTSADLPRFSQYLSAFGAGAEDTLYMPTLRVHKGLP
                     FGIDVGLMYAKVPSSNIKYTGGELKWAIIDGNTVLPAVAVRGSLTKLSGVDQLSLDTQ
                     GFDVSVSKGLAMFTPYAGLGRVRVTSTPIGVPLAEEKFSQTKVFAGVGLNLALVNLNF
                     EYDKTGEVKSYSAKFGLRF"
     gene            complement(9719..10540)
                     /locus_tag="VIS73_01835"
     CDS             complement(9719..10540)
                     /locus_tag="VIS73_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761929.1"
                     /translation="MTDETAIPIDPEDLAALGERILELPDEQATWVMALYQECVRARL
                     AEAEHLASTLDATAQVQEIAQDVAQIVLDAAEWLRTLWEVGYMGGGQLPASPRTRFPQ
                     IEVEDVLKSVLLARIRSGLRPLPFPPPTRNGVPWHEIVEAEREATVDASPIDDAGTTI
                     AFAIDGDTGWDVLAQIDPDRYRVQHRGKGPLYQLDLSNSPALLSREAPTIERTIERHE
                     RAGIQHFTLQWIEADGKERSIPLRAASPDRAEIEAQHWIAREHPERYGQVRFVLN"
     gene            complement(10552..11610)
                     /gene="mnmH"
                     /locus_tag="VIS73_01840"
     CDS             complement(10552..11610)
                     /gene="mnmH"
                     /locus_tag="VIS73_01840"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEY5761930.1"
                     /translation="MKNPAITTVDSLDGFDAIIDTRSPAEFAEDHLPGAINCPVLDDA
                     QRAEVGTLYKQHSPFAARRVGAALVSENIARHLRTAFADQPKQWRPLVYCWRGGQRSG
                     AMTIVLRQVGWDARQLEGGYKAYRRRVVTELAALPLPLDFIVIGGPTGSAKSRVLQAL
                     AERGEQTLDLEALASHKGSVLGLLPGLAQPSQKAFESALWQALGRLDPARPVFVEAES
                     RKIGRLQVPEVLIERMRAARCVAIDAPLEARVEFLIGDYDYMVRDPEHLIGRLDALRP
                     LRGSEPVDGWIELVRAARWSDLVECLLTGHYDPLYRKSQELNYRAHGAPPSVTAQRLD
                     AAGIEAVAAAIIATHNTP"
     gene            complement(11607..12128)
                     /locus_tag="VIS73_01845"
     CDS             complement(11607..12128)
                     /locus_tag="VIS73_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY5761931.1"
                     /translation="MLGDSLSAGYGIDRDQAWPALLQARLGAEYSVVNASISGETTSG
                     GRARLPQLLARHRPHLVIVALGANDGLRGLPLQQMRDNLDAMIRQNVQHQSRTLLVGI
                     RMPPNYGAYADAFARVFDELAQTRKVPLVPFLLEGFADQVAYFQADGLHPTAAAQPLI
                     LDNVVPSIRAALR"
     gene            12238..12987
                     /locus_tag="VIS73_01850"
     CDS             12238..12987
                     /locus_tag="VIS73_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5761932.1"
                     /translation="MSEKGILEVRSLGKRIDTGGKALVILDDVSFSVTVAESVAIVGA
                     SGSGKSTLLGLLAGLDTPSSGEVWVDGENLSALDEDARASLRGRLLGFVFQSFQLLPA
                     LTALDNVMLPLELAGQSKAAETAREWLGRVGLSARAHHLPRELSGGEQQRVALARAFC
                     TNPRLVLADEPTGNLDAATGTQIIDLMFSLNAELKTTLVLVTHDEAIAARCARRLRMV
                     EGRLFDEGEAPRVGLKTGLAIDFATKAVTID"
     gene            13018..13500
                     /locus_tag="VIS73_01855"
     CDS             13018..13500
                     /locus_tag="VIS73_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY5761933.1"
                     /translation="MADNVYVFKATTLDGKACSLSEFEGRVALVVNVASRCGFTPQYE
                     ALEKLWRQYRDRGLVVLGFPCNQFGAQEPEDEAGIAKFCQQNYGVSFPMFAKVKVNGD
                     EAHDLFRYLTKALPGVMGSTAIKWNFTKFLIGRDGVPRRRFAPATTPEEIIPEIERLL
                     "
     gene            complement(13497..14867)
                     /gene="hpnE"
                     /locus_tag="VIS73_01860"
     CDS             complement(13497..14867)
                     /gene="hpnE"
                     /locus_tag="VIS73_01860"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEY5761934.1"
                     /translation="MLDTGRKQPPRVAIVGAGWAGLAAAVTLVERGAVVTLYEAGRVA
                     GGRARGIELDNIRVDNGQHLLIGAYRRTLRLMRRVGVDPDTALRRLPMRVLVPGRFDL
                     RLPRLPAPLHTAVGLLSVRGARWREIGSAVGAMRRLQASAYRLEADLPVSDWLDSTGQ
                     TGAIRRHLWEPLCLAALNTPPGSASAQVFANVLRDTLGGRRRDTDFLLPRHDLGKVLP
                     EPATRWLAARGARLRYGCRVRALEANGCRWRVHSDMPGASAGVSGNDVDHAYDQVVLA
                     VGPQHASALLPPSPRIESLREGLAAISYQPIATVYLRYPGRHRLPHPILLLPGPTGQW
                     VVDRSALDDGSEQNGSLIAHVLSAHGDWEAHSDEELGRRLHAELRAVQPGLPAMQSCR
                     VIREKRATFACTPDLWRPPQLSGLKGLWLAGDYTRSDYPGTLEAAVRSGEAVAEAIAG
                     VQSSSV"
     gene            complement(14868..15701)
                     /gene="hpnD"
                     /locus_tag="VIS73_01865"
     CDS             complement(14868..15701)
                     /gene="hpnD"
                     /locus_tag="VIS73_01865"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEY5761935.1"
                     /translation="MNPDEYCSEKARAAGSSFTYSFRLLPPARRRAITALYAFCREVD
                     DVVDECLDPQVAATKLAWWRHEVDQLFAGKAQHPVTRALAPAIEEFSLPQEHFAEILD
                     GMAMDLGSVRYPDFKSLSLYCYRVASVVGLLSAEIFGVSDRQTLRYAHDLGIALQLTN
                     IIRDVGDDARRGRIYLPLDEMARHAVTPADILNRRYSEGFAALMGVQADRALDYRQRA
                     LAALPAADRKAQRPGLVMAEMYRCLLEEIRLSGYQVLHQRISLPPARKAWIAMRTWVA
                     A"
     gene            complement(15711..>15963)
                     /locus_tag="VIS73_01870"
     CDS             complement(15711..>15963)
                     /locus_tag="VIS73_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761936.1"
                     /translation="DHEIERTRALMLAGAPLLAVLRGRMRWEIRATVNGGLRILDRLQ
                     LIRGDVFARRPTLGWRDWLWIGSQTTIGEPRSMPLAGHG"
BASE COUNT         2867 a         4813 c         5499 g         2784 t
ORIGIN      
        1 cgttcaagaa ggacggttcg gtaaccgccg gcaacgcttc cgggatcaac gacgccgcgg
       61 ccttcctcgt gctcgccgat gcggcgaaag cctcggctgc tggctacaag ccgatggcgc
      121 ggctggttgc ctacggcatc ggcggcgtgc cgaacgagat catgggcgag ggtccgattc
      181 cctcgtcgaa gattgcgctc gccaaggccg gcctgaagct cgaccagatg gacgtgatcg
      241 aatcgaacga agcctttgcg gcgcagtcgc tggccgtcat taagggcctc ggaatcgatc
      301 cggcaaagac caacgtcaat ggcggcgcga ttgccattgg ccacccggtc ggcgccaccg
      361 gtgccgtgat cgtcacgaag ctcctgcacg agatgcagcg gacgaaggcc aagtacggca
      421 tggcgacgat gtgtatcggc ggtggtcagg gcatcacgac gatttacgag cgcctctgat
      481 cgcggctcgg gttcgaaccg aaacggcgag cgcaagctcg ccgtttttca tttcggcagc
      541 tcgtcgcata gtcggtaata gctggccatt cgcaccgggt ctatttcgcc gcgggccttg
      601 gcttcgagca gcgcgcaatt gggttcgtgc cggtgacaac aatcgcgaaa ccgacagttg
      661 ccgagatgtg ggcggaattc gacgaagctt tcgctgagcg tctgccgatc aagatgagcg
      721 aggccgaatt cctggaggcc gggagagtcg atcagcgcgg tgtccgcgtc gagcgtgtag
      781 taggttgcgt gggtcgtcgt atggcggccg ctgccgagcg cgaccgagat ttcccgggtg
      841 gcagccatcg cgccgggcac cagcgcattc accaatgttg atttgcccat ccccgactgg
      901 cccacgagaa cgctggcttc acctgccagc agcggaagca gcgcggacgc gtcgcatttc
      961 gcactgatct cgagtatgcg cgtgccgatt ttcagcagag gttgcagcag gctacgcgcg
     1021 gggtcgatgt gctcgcgcag gtcacacttg ttgaggacga ccagtgcctt gagcgattgg
     1081 ctttcggcgg caatcaggca gcgactgacc agatcgatcg agaaccccgg cgtcgttgcg
     1141 acgacgatga ctatctgggt ggcgttggcg gcaatcagtt tctggcggta cggatcggag
     1201 cgatagagca gactcgttcg cggcaggcac gcgacaacga ctgcttgatc ttccgactgc
     1261 cgttggatgc gaacccgatc gccaaccgcc agctcgcttt tcttgccgcg gggaaaacag
     1321 ttgatcgact cgccgtcatc ggtcagtacg cggtactgcc gcccatgcgc ggagataacc
     1381 cggcctacgt attcaccgct gtgtccggcg tttattggtc gaatcaagtg gccgcccgga
     1441 ttcgcgcgat tcgttccatt gccggcgggt gcgagtcgaa gaagcgtgaa tggtagcggt
     1501 cggggtccaa tgtcgcggcg ttgtcgcggt agagctggat cagcgccgac tggaggctgt
     1561 cagccgacgt ctggttcacc gcgtaacggt cggcctcgaa ctcgtcccgg cgtgagagac
     1621 cgttgaacaa cggttgcagg ggaaacagca gcaagggaaa gaacagcgag aaggcgatga
     1681 gcgctgcggc gtcggagaca cggttgacgc cgaggcccga gaaaaaccag ggtgactcgc
     1741 gcagggtcgc catgagggcg agcacgaggc cggcaccgag gattgccagc gcgacgcgct
     1801 tcaggacatg ccggtggtgg aaatggccaa gctcatgcgc cagtaccgcc tcaacctgcg
     1861 ccggcgaaag tttctccagc aaggtatcaa agaagacgat ccgccgcttg ttgccgacgc
     1921 cggtaaagta ggcgttgccg tggctcgatc gcttcgaacc atccatgacg aagaggccac
     1981 cggcgcggaa gccgcagcgc gtcagcaacg cctcgattcg ggcggcgagg ctggcgtcgt
     2041 caagggggcg gaaactgttg aagagcggtg cgatgaaggc cgggtacagc cagagcaaca
     2101 gcagattgaa gccgctccac agtgcccaga cccaaagcca ccagttggcg ccggtactgg
     2161 ccatcaactg cagtgccgcc caggccagcg ggatgccgag tagggctgag agcaaagcgg
     2221 ccttgcccag gtcagcgaga aacagcgctg gcgtcatgcg gttgaagcca tggcgtgcct
     2281 caatcacgaa ttgccgatgc caggcaaagg gcaggtccac gatcgcgtta acgagggcca
     2341 gcagcaccag caggatgatg ccgccggcga ttgccgagga agtaagggct gctgacaacc
     2401 cggaaagcag atcgagtccg ccgccaaagg taagcgccat taccagcgcc gcatcactta
     2461 ccgttcccca gcgcgagaaa cgggccttgt cgactccgta atccacggct ttggcgtttt
     2521 cctgggcaga cacttcggcc gcgaaaaccg caggcggttg atcgcggctg ccctggagct
     2581 tggtgatctg ccggttggcg agccagagcc gcagcgtcag cgcaagcagc cagaacgcgg
     2641 caaaggtagt tgcggtcagc gtttccaaac tcgggtaggt gcccatgctg tgcgaaaatg
     2701 gcgcgttgtc taggagagaa aaattatggc acaggacgca agccggctcg tctggctgga
     2761 catggaaatg accggattgc agcccgacag cgatcggatc atcgaattgg caatcgtcgt
     2821 gaccgacgag tcactcgtga ccgtcgccga atcgccggtc tgggtcgtgc atcagagtga
     2881 cgtggtgctc gatggcatgg attcgtggaa caagggcgcg catggacgct cgggcctgat
     2941 cgaaaaggtt cgcgcttcga cgctcgacga agccgcggtc caaaccgcgg cgctggcctt
     3001 tctcaaggag caggtgccaa agcgcgccag cccgatgtgc ggcaattcga tctgccagga
     3061 ccgacgcttc atggcgcgct atatgccgga gctggaagcc tggttccatt accgcaacct
     3121 cgacgtcagc acgatcaagg aactctgcaa gcgctgggcg ccggcgctgg catcggggtt
     3181 cagcaagcac aaccgtcacg aggcgcttgc cgacatatac gaatcgatcg aggagcttcg
     3241 ctactatcgc ggctgctttc tcaaggtcgc cagcgacgag ccgcaatcgg cctagccgcg
     3301 gtggtagagg cgcagccgct cgtggcgatc gcccgggcgg ttgccctccc agaccttgcg
     3361 ccattcgggt tcgggcggaa actcgtcgag gcttccctga atcagcaggt aacggcaatc
     3421 aggtcgcgcc tcgggattga ggcggcgatt catcgcgaat tcgatggaac tcagttgcgc
     3481 gggcgcgacc gcacgcggct caatgcagga tttgccggaa tgcgccggtc cgagagccgc
     3541 aagcaattcg gtgccaaccg gacgatagct tttcaggtgc tcgatccacg gtagccagag
     3601 ggacatggcc aggcaccaga cgagggtaat tccggcggtc caatgcccga tgctgcgcag
     3661 cggtgagcgc cgttcgcgga ccacgaacca gagccagagc ccggtaatcg tcagggcgaa
     3721 gccggcggcg agccagcctg tctgcggttc gaatccgggc gtcaggcgca ggatgttgtg
     3781 cgagaccttg ggcggaagct gaaactgcat cgcgaaccag ccgaaccaca gcaggaaggc
     3841 gagcagtccg aaggtaagtc gaccgaacca gtcgaaggca tgagccgcgc cgcgccgcag
     3901 ttgcgggacg ccctgcgcgg cgagcagggc gagtggtggc agcagcagca gcgccggcgc
     3961 agccctggca ctttcgatcc aagcgatggc gatccagagt acgaagaagg aaatcgcacc
     4021 gagccagatt gctgatgtcc gccaggcatg gcgccgcgac cacaatcccc agaccgcgag
     4081 cggccacgcg ggccaggcga accagccgag ctggttgagg tagcggaagg cgttgcgcgg
     4141 aaagtcggag acgcctgcga gctgagccag ttcggcgcca agaaagacgg ccagctgcgt
     4201 cgagccggcg cgcgccagcg ccaggtacca gggaagtgcg acgaccgcgg cgcagagcag
     4261 cgcgatgagg atgcgcacgc cggttgccag gcgctgcgag cgttcggcgg ctaccagcgg
     4321 tgaggcgagg atgaggagcc ccgcgggcag cagtgtcagg ccgtccgcaa ggactaggcc
     4381 ggccagtccg gcggccagca ttggcgttgc atagcgctgc cggttatgca gtgctgccac
     4441 acccgccagc agcgcacaga tcgccgcgac gactgccaga aggggctggg tttcgtggga
     4501 ttgcaccagg aagcccaggc agccagcgag ggccagcggc gcggccgggg cggcttcctt
     4561 gccgaacaac tcggtcgcgg caagatagat gagcgcgcag gcggcgccag caaacagcag
     4621 ggacgcgatg cgaaaggcgt cgggcagtcc gaggctcgag ccaaaaagga cgccgcagac
     4681 ccgcgcgacc cagaagtaga gggggggcgc aggtccggtc gcctcggcag cgctgaatag
     4741 aagccactcg gaatccagcg cggcgcgcca gatcgtgccg aaatggatcg catcttcgct
     4801 cttccaggga tcgtggccga gtatgccgct cgccagaaac agcgcgagca ggacgacgag
     4861 gggaatgccg cgcgtcagcg gcggcggcgc gtagcggcgc tggcgggtgt cgtgcatggg
     4921 cagaatctaa acgaaaaagg cagcctcagg ctgcctttgg gtgcgaagcg cgcagtgtcg
     4981 cgatcaggat ggcttgcgca gcgaaccgaa tttctgattg aacttctcga cgcgtccggc
     5041 cgtgtcgacg atcttctgct tgcccgtgta gaagggatgg cattccgagc agacttcgac
     5101 ctgaagcggc ttgccgagcg tcgagcgggt cgtgaacgcg acgccacaac tgcaggtgac
     5161 ctgcacatcc ttgtagttcg ggtgaatctc ggctttcatc ttcaatgtcc ttcaaatgcg
     5221 gccggcggat ttggccggcc cggaaaaggg ggcgattatc ccgaaacgcg gcgccggatg
     5281 caagtcccgg ctgagttgag cagggggcgc cgcgacggtg tctagcgacg cattgcgtcg
     5341 aagaactcgg cgttgttttt ggttgccttg atcttgtcaa gcaggaattc catcgcttca
     5401 agatcgtcca tgttgtagag gagctttcgc aacagccaca ttttctgcaa tacggccggc
     5461 tcgagcagca gttcctcgcg acgcgtgccc gagcgattga cgttgatcgc cgggtagacc
     5521 cgtttctcgg ccatccgccg gtcgaggtgg atttccatgt tgccggtgcc cttgaactcc
     5581 tcgtagatca cgtcgtccat gcggctgccg gtgtcgatca gcgcggtcgc gatgatggtc
     5641 agcgagccgc cttcctcgac gttgcgtgcc gcaccgaaga agcgcttggg cttctgcagg
     5701 gcattggcgt cgacgccgcc ggtcagcacc ttgcccgaag caggttgaac ggtgttgtag
     5761 gcgcgcgcca gtcgcgtgat cgaatcgagc agaatgatga cgtcgcgctt gtgctcgacc
     5821 aggcgcttgg ccttttcgat gaccatttcc gccaccccga cgtggcgcga agccggctcg
     5881 tcgaaggtcg aggcaaccac ttcaccgcgg accgtccggc tcatttcggt aacttcttcg
     5941 gggcgctcgt cgatcagcag gacgatcagg gcggcgtccg gatggtttgt tgtgatcgcg
     6001 tgggcgatgt gctgcagcat cacggtcttg cccgatttcg gtgaggcgac tagcagaccg
     6061 cgctggccgg caccgattgg cgcgatgatg tcgatgatgc gcgaggtggt gttctcctcg
     6121 ctgcggatat cgcgttcgag tcgcagatgt cgcgtcgggt gaagcggcgt caggttctcg
     6181 aagagaatct tgttcttggt tgcctcgggc gactcgccgt tgatcttgtc gagcttgacc
     6241 atcgcgacat agcgctcgcc ttccttcggc gtgcgaatct ctccttcgat ggtgtcgccg
     6301 ctgtgcaggt tgaagcggcg gatctgcgat ggcgagacgt aaatgtcgtc ggtgccagcc
     6361 agatacgagg tgtcgggtga gcgcagaaaa ccgaatccgt cgggaaggac ttcaagcgtt
     6421 ccttcgccga aaatgctctc gcctttcttg gcgcggtcct tgaggatcgc gaagagcaat
     6481 tcctgcttgc gcagacggtt ggcaccttcg atgccgctgg tcaaagccat ttcaagcaat
     6541 tggctgacgt ggaggttctt gatttctgag agttgcatgg tctgcggatg gcgttacgga
     6601 gagcggaagg gggcaggtcc ggaggtggcg cgaatcggac tgctaggggt gctggccggt
     6661 cagtggttcg gccagaagga acagcttgga atcaggggtg gcgcggggcg ctacaggatc
     6721 ggatcgatca gcgcgttctg ccgatcagag cagaacgcgg acgagattag cccagtcaga
     6781 gattgctgtc aatgaaggcg gcgagcgccg acttcgacag ggcgccgacc ttggtggctt
     6841 cgacattgcc gttcttgaac agcatcagcg tcggaatgcc gcgaatgccg tacttggccg
     6901 gaacgttctg gttgtcgtcg atattgagct tggcgacctt gaggcgccct gagtattcgc
     6961 tggcgatttc gtcaaggatc ggtgcgatca tcttgcaggg gccgcaccat tcggcccagt
     7021 agtcgaccag aaccggctgc gccgacttga ttacctcggt atcgaaactg tcgtcggtca
     7081 cgtagtgaat gtgttcgctc atcggaaaaa atctcgctgg atgggaaaag gatgttggcg
     7141 gtgcccgcgg aaccaccgcg tctggagcgc ggtctcggtt caggtcggac aatgctaccc
     7201 caaccgcggc gcctcgccta gcgccagaca gttgcccgga tggcgtagtt ccaccaattg
     7261 gaaaccgacg cggccgcagt ttgtccaagt cgcaattccg caggagggcc aatgacctac
     7321 gttgttaccg agaactgcat caagtgcaag tacacggatt gtgtcgaagt ttgccccgtc
     7381 gactgtttcc atgagggtcc gaatttcctc gtcatcgatc ccgaggaatg catcgattgc
     7441 acgctttgcg tcgcagaatg ccccgcggag gcgatttttg ccgacgccga cgtcccggaa
     7501 tcgcagcgct cgttcatcga cctaaacgcc gaattggcca agtcctggcc agtcatctcg
     7561 gcacgcaagg aaccggccga ggatgcagat gcctggctcg gcgtacccgg caagctcgat
     7621 cagctcgaac gctaggaggc cttgccgcga atcctgtcaa gggcatcggc gagccgtgcg
     7681 accgtcgccg ggacgtcgcg gagtttatcg aggccgaaca ggccaatgcg gaagctgcgg
     7741 aagctttcac cctcgtcgca catcagcggc acgccggccg ctgtttgcag tccgagcccg
     7801 agaaacttcc ggcccgactg gatttcgggg tcgctggtgt agctgacgac gaccccgggc
     7861 gcctcgaagc cggccgccgc gacgctgggg tatccacgct gcgccagcag cgagcgaacc
     7921 gcacggccca acgcctcctg cgcgtcgcgg gccgccgcca gccccatctg ctcggtttca
     7981 agcatggctg cgcaggacag tttcagcgca tcggtgggca tggtcgcgtg gtaggcgtgg
     8041 ccgccgttct cgtaggtttc catgatctgc agccattttt tcaggtcgca ggcgaagctc
     8101 gaactggtcg tcgcatcgat cgccttgcgc gcggtctcgc ccagcatcac gaaggccgcg
     8161 cccggagagc cgctccagcc cttctgcggg gcgctgacca ggatgtccac gccgcagccc
     8221 ttcatgtcga cccagacggc gcccgaggcg atgcagtcga gcacgaacag gccgccgtag
     8281 ctgtggacgg cttgggcgat ttgtttcagg taatcatcgg gcagcaggat tcccgaggcg
     8341 gtttcgacat gcggcgcaaa gaccagatcg ggcttttcgg caaggatcgt cgccaccacc
     8401 gcttcgactg cgaccggcgc gaaaggcgcg gtcggctcgg gcgatgtctg ccgggccttg
     8461 agcacggtcg tcgccgaggg aatctttccg gcgtcgagga tctgggtcca gcgatagctg
     8521 aaccagccat tgcggatgac cagacaacgt cttcccgtcg cgaactggcg ggcgatggct
     8581 tccatgccat aggtgccgcc accggggacg acggcgcagg cctgtgcgcc ataggtcgtc
     8641 ttgaggatgc gcgacagttc ggtcatcacg ccctgaaaac gcttcgacat atggttaagc
     8701 gcacggtcgg tatagacaac cgagaattcg aggtggccgt ctgggtcgac gttgggtagc
     8761 agtccaggca cgtgggatga ctccgtcaat aggggtgaag gcgaatttca gcggcggcgc
     8821 gggcgcaaac cagcgctgtt gccggaagcg tcgactcaga catcgacagg cggcaaggac
     8881 acgcgttccc gccaataagc caggaacgcg aggaatccgg ctggatcaga agcgcaggcc
     8941 gaacttggcg ctgtaggact tcacctcgcc ggtcttgtcg tactcgaagt tcaggttgac
     9001 cagcgcgagg ttcagcccga caccggcgaa gaccttggtc tgcgagaact tctcttcggc
     9061 tagcggtacg ccgatcggcg tgctggtgac gcgtacgcgt ccgagcccgg cgtagggcgt
     9121 gaacatcgcc aggcccttcg ataccgagac gtcgaatccc tgggtatcga gcgacagctg
     9181 gtcgacaccc gacagcttgg tcaggctgcc gcgcaccgcg acggccggca ggaccgtgtt
     9241 gccgtcgatg attgcccact tcagttcgcc gccggtgtac ttgatgttgc tgctcgggac
     9301 cttcgcgtac atcaggccga cgtcgatgcc gaagggcagg cccttgtgca cgcgcagggt
     9361 cggcatgtag agcgtgtctt cggcgccggc gccgaaggcc gacaggtatt gcgagaagcg
     9421 cggcagatcg gccgaggtga cggcgatgcc gatgtcgaag ccggtgatgc cgagcggctc
     9481 ggcgggaatc tggccgtgat agctcaaggt tgctccgata tcttcggaaa acagccggaa
     9541 ctggctctgg gcgaggctgc cgacgtcgtt gatgtcttcg gcgagggcgg ctgaggtcat
     9601 tcccagcgaa agggctgcag cgatcaggtg acggcgcacg gtgtttctcc tggatggaaa
     9661 ggtcaaaagc tggattctat tcacataagt ggatgttgtc tttggcagtc gatgcgtctc
     9721 agttcagcac gaaacgcacc tggccgtagc gttccgggtg ttctcgcgca atccagtgct
     9781 gagcctcgat ctcggcgcgg tcgggactgg cggcgcgcag cgggatactg cgttccttgc
     9841 catctgcctc gatccattgc agcgtgaagt gctggatgcc ggcgcgttcg tggcgctcga
     9901 tcgtgcgctc tatcgttggc gcttcgcggc ttagcagagc aggcgagttg gagaggtcga
     9961 gttgatagag gggtcccttg ccacggtgct ggacgcgata acgatcggga tcgatttgtg
    10021 ccagaacgtc ccagccagtg tcgccgtcaa tggcgaaggc gatcgtcgtt cccgcgtcgt
    10081 ctattgggct ggcatcgacg gtggcttcgc gctcggcttc gacgatctcg tgccagggca
    10141 cgccatttcg cgtcggcggc ggaaaaggca ggggccgcag gccgctgcgg atgcgcgcga
    10201 gcaagaccga cttgaggacg tcctcgactt ctatctgcgg aaagcgcgtg cgcggcgatg
    10261 ccggcagttg gccgccgccc atgtagccga cttcccagag ggtgcgcagc cactcggcgg
    10321 cgtcgaggac gatctgcgcg acgtcctggg caatttcctg cacctgcgcc gtcgcatcga
    10381 gggtgctggc cagatgctcg gcctcggcga gccgcgctcg aacacactcc tgatagagcg
    10441 ccatgaccca ggtcgcctgt tcgtcgggca gctcaagaat ccgctcgccg agcgcggcga
    10501 gatcttcggg gtcaatcggt atcgcggttt cgtcggtcat ggggcctcgc tctagggcgt
    10561 gttgtgggtg gcgatgattg ctgccgccac ggcttcgatt ccggcggcgt caaggcgctg
    10621 ggccgtaacg cttggcggcg caccgtgcgc tcggtagttg agttcctgcg acttgcgata
    10681 gagcggatcg tagtggccgg tgagcaggca ttcgacaaga tcgctccagc gcgccgcacg
    10741 aaccaactcg atccagccgt cgacgggttc gctgccgcgc agcggacgca gcgcgtcgag
    10801 tcgaccgatc aggtgctcgg gatcgcgaac catgtagtcg tagtcgccaa tcaggaattc
    10861 gacgcgagct tcgagtggcg cgtcgattgc cacgcagcgt gcggcgcgca tgcgttcgat
    10921 cagtacttcg ggaacctgca ggcggccgat cttccggctc tcggcttcga cgaacaccgg
    10981 gcgcgccggg tccagacgtc cgagcgcctg ccacagcgcg gactcgaagg ccttctggct
    11041 tggttgggcg agaccgggta gcaggccgag gaccgagcct ttgtggctgg ccagcgcttc
    11101 gaggtcgagc gtctgctcgc cgcgctcggc cagcgcctgc aacacacggc tcttggcgct
    11161 gccggtcggg ccgccgatga cgatgaagtc gagcggcaac ggcagggctg caagctcggt
    11221 gaccacgcgt cgccgatagg ccttgtagcc gccttcgagt tgccgcgcgt cccagccaac
    11281 ctggcgcagg acgatggtca ttgcgccgct gcgctgtcca ccgcgccagc agtagaccag
    11341 cgggcgccat tgtttcggct ggtcggcgaa ggcggtgcgc aggtgccggg cgatattctc
    11401 ggaaaccagc gcggcgccga cgcggcgcgc cgcgaacggc gagtgctgct tgtacagcgt
    11461 gccgacctcg gcgcgctggg cgtcgtcgag gaccgggcag ttgatcgcgc cgggcagatg
    11521 gtcctcggcg aactccgcgg gcgagcgcgt gtcaatgatg gcgtcgaagc cgtctagcga
    11581 atcgacggtc gtgatggctg gattcttcat cgcagcgcgg cgcggatcga cggcaccacg
    11641 ttatcgagga tcagcggctg tgcggcggcc gtcggatgca gaccgtcggc ctgaaagtag
    11701 gcgacttggt cggcgaagcc ctcgagcagg aaaggcacca gcgggacctt gcgggtctgc
    11761 gcgagttcgt cgaagacgcg tgcaaaggcg tcggcgtagg cgccgtaatt gggtggcatg
    11821 cggatgccga cgagcagcgt gcgcgactgg tgttgaacgt tttgccggat catcgcgtcg
    11881 aggttgtcgc gcatctgctg cagcggcaag ccacgtaggc cgtcattggc gccgagcgcg
    11941 acgatgacta gatgtgggcg atggcgggcg agcagttggg gtaggcgggc acgcccaccc
    12001 gaggtggttt cgccggagat gctggcattg acgacgctat actcggcgcc gagtcgcgct
    12061 tgcagcaagg ccggccacgc ctgatcgcga tcgatgccat agccggccga gagcgaatcg
    12121 ccaagcacca gaattcgctt tgccggcgcc gcgaacgcgg cgctggcgag gcagaggacg
    12181 acaatcggca gcagcctgcc gatggatcgg acaagcgcaa caagcgatgg ggatgcaatg
    12241 agcgaaaaag gcatattgga agtcaggtca ctcggcaagc ggatcgacac gggcggcaag
    12301 gccctggtca ttctcgacga cgtctcgttc tcggtcacgg ttgcggaaag cgtcgcgatc
    12361 gtcggcgctt ccggttccgg caagtctacc ctgctgggcc tgcttgccgg tctcgacacg
    12421 cccagctcgg gcgaggtgtg ggttgacggc gaaaacctga gcgcgctcga cgaagacgcc
    12481 cgggcgagcc tgcgtggccg cctgctgggt tttgtcttcc agtccttcca attgctgccg
    12541 gcgctgaccg cgctcgataa cgtcatgctg ccgctggaac tggccgggca gtcgaaagcg
    12601 gcggaaacgg cccgagaatg gctcgggcgg gttggtttgt cggcgcgggc gcatcacctg
    12661 ccgcgtgagc tgtcgggcgg cgagcagcag cgggttgcgc tggcgcgggc cttctgtacc
    12721 aatccgcgac tggttctcgc cgacgagccg accggcaatc tcgatgccgc gaccggtacg
    12781 cagatcatcg atctgatgtt ctcgctcaac gccgaattga agacaacgct ggtcctggtg
    12841 acccacgacg aggccatcgc cgcgcgctgc gctcgccgcc tgcgcatggt agaagggcgc
    12901 cttttcgatg aaggcgaggc tccgcgcgtc ggcctgaaaa cggggttggc catcgatttc
    12961 gccacgaaag cagtgactat cgactaggct gaggttgttg gcgatgaaag gaatgcgatg
    13021 gcggacaatg tctatgtctt caaggccacc accctcgatg ggaaggcgtg ctcgctttcc
    13081 gagttcgagg gccgcgtcgc gctggtggtc aacgtcgcca gccgttgcgg cttcacgccg
    13141 cagtacgagg cgctcgagaa attgtggcgg cagtatcgtg accgcggcct ggtcgtgctg
    13201 ggtttcccct gcaaccagtt tggggcgcag gaacccgagg acgaagcggg aatcgccaag
    13261 ttctgccagc agaactacgg ggtgagcttt ccgatgttcg ccaaggtcaa ggtcaacggc
    13321 gacgaggcgc acgatctgtt ccgttatctg accaaggccc ttcccggcgt gatgggcagc
    13381 accgccatca aatggaattt caccaagttc ctgattggtc gcgatggcgt gccgcggcgt
    13441 cgcttcgcgc cggcaacgac ccccgaggaa atcattcccg aaatcgagcg cctgctctag
    13501 acgctcgagg actggacgcc ggcgatcgct tcggcgacgg cttcgccgct gcgcactgcg
    13561 gcttccagcg ttcccgggta gtcgctgcgc gtgtagtcgc cggcgagcca caaccccttg
    13621 agaccgctga gttgcggcgg tcgccagaga tcgggcgtgc aggcgaaggt tgcgcgcttc
    13681 tcgcggatga cccggcacga ttgcatcgcc ggtaaacctg gttgcacggc acgcagttcg
    13741 gcgtgcagac gacggccgag ttcttcgtcg ctgtgtgcct cccagtcgcc gtgggcgctc
    13801 agcacgtggg cgatcaggct gccgttctgc tcggaaccgt cgtcgagcgc gctgcggtcc
    13861 acgacccact ggccggtggg accgggcaga agcaggatcg gatgcggcag gcgatgccgt
    13921 cccgggtagc gcaggtagac ggtggcgatc ggctgatagc tgatcgccgc gaggccttcg
    13981 cgcagcgact cgatccgtgg cgatggcggc agcagggcgg aagcgtgctg cggaccgacg
    14041 gcgagcacga cctgatcgta agcgtggtcc acgtcattcc ctgaaacccc ggccgaagcg
    14101 cccggcatgt cgctgtgaac ccgccaccgg cagccatttg cctcgagggc gcggactcga
    14161 cagccatagc gcagccgcgc gccgcgcgcg gcaagccagc gcgtcgccgg ttccggcagg
    14221 actttgccga ggtcgtgacg cggcagcaga aaatcggtgt cgcggcgacg gccgcccagc
    14281 gtgtcgcgca ggacgttggc gaagacctgg gccgaggccg atcccggtgg cgtgttgagg
    14341 gcagccaggc agagcggttc ccacaggtgc cggcggatcg ccccggtttg cccggtcgaa
    14401 tcgagccagt cgctgaccgg gagatcggct tcgagtcggt aggcgctggc ctgcagccgg
    14461 cgcatcgcgc cgacggcgct gccgatctcg cgccagcggg cgccgcgcac cgagagcagg
    14521 ccgacggcgg tatgcagggg tgccggcaga cgtggcagcc gcagatcgaa acggccgggc
    14581 acgagtacgc gcatcggcag gcgccgcagc gccgtgtcgg gatcgacacc gacgcgccgc
    14641 atcaggcgca gcgttcggcg ataggcgccg atcagcagat gctggccgtt gtcgacgcga
    14701 atgttgtcga gttcgatccc gcgcgcccgg ccgccggcaa cgcggccggc ttcgtagagc
    14761 gtgaccacgg cgccgcgttc gaccagcgtc accgccgccg ccagccccgc ccagccggca
    14821 ccgacgatcg cgacgcgggg cggctgtttg cggccggtgt cgagcatcta ggccgcaacc
    14881 caggtgcgca tcgcgatcca cgccttgcgc gccggtggca gcgaaatgcg ctggtgcagg
    14941 acctgatacc cggagaggcg gatctcttcg agcaggcagc ggtacatctc ggccatcacc
    15001 agtcccggcc gctgcgcctt gcgatcggcc gccggcagtg ccgccagcgc gcgctgccgg
    15061 tagtcgagcg cgcggtcggc ctgaacgccc atcagcgccg caaagccttc gctgtagcgg
    15121 cggttgagga tgtcggccgg cgtgaccgca tggcgcgcca tttcgtcgag cggcaggtag
    15181 atgcggccgc gccgcgcgtc gtcgccgaca tcgcggatga tgttggtcag ctgcagcgcg
    15241 atgccgaggt cgtgcgcgta gcgcagcgtc tgccggtcgc tgacgccgaa gatctctgcc
    15301 gagagcaggc cgaccacgct ggcgacgcga tagcagtaga gcgagagtga cttgaagtcg
    15361 gggtagcgga cgctgccgag atccatcgcc atgccgtcga ggatttccgc gaagtgttcc
    15421 tggggcaggc tgaattcttc aatcgccggt gcgagcgcgc gggtaaccgg atgctgcgcc
    15481 ttgccggcaa acagctggtc gacctcgtga cgccaccacg ccagcttggt ggcggcgacc
    15541 tgcgggtcga ggcactcgtc gacgacgtcg tcgacttcgc ggcagaaggc gtagagcgcc
    15601 gtgatcgcac ggcgccgagc cggcggcagg aggcggaagc tgtaggtaaa gctcgacccg
    15661 gcggcgcggg ctttttcgct gcaatattcg tcgggattca tcggtcgatt ctatccgtgt
    15721 ccggccaatg gcattgagcg cggctcaccg atggtggtct ggctgccgat ccacagccag
    15781 tcgcgccagc caagcgttgg ccgccgcgca aagacatcgc cgcgaatgag ctgcagacgg
    15841 tcgagaatgc gcaggccgcc gttgacggtg gcgcggattt cccagcgcat gcggccgcgc
    15901 aggacagcca gcaacggcgc gccggcgagc atcagcgccc gcgttcgctc gatttcgtga
    15961 tcc
//
LOCUS       DATDAA010000054         2166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_213570,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000054 DATDAA010000000
VERSION     DATDAA010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213570"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..>2166
                     /locus_tag="VIS73_01875"
     CDS             14..>2166
                     /locus_tag="VIS73_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5761937.1"
                     /translation="MLSLAVIIVSGVITYIGVPKEAEPDLKIPMVYVSMTHDGISPED
                     AERLLVRPMEQEIRTIEGIKELSSNAYEGGASVLIEFDAGTDVDVALQDARAKVDAAK
                     AELPSETEEPTVTEIRFSREQPMLVLNLAGDVPERTLNTIARDLKESIESLPGVMEVT
                     LVGVREELMEIVVDPRAMESYGLDQAQILTLIDRNNRLVAAGALHTSEGRFPVKVPGV
                     FETAEDVLEMPIKAVGERVVHFRDVAEVRRTYKDAESVARLNGDRALAIEVVQRSGAN
                     IIETVAAVNAIVEQARAAWPGGIQVFASSDKSKDVRDMLSNLQNSVITAVLLVFIIIV
                     GILGIRSAGLVGISIPGSFLLGILTIASFGITINMMVLFALIMAVGLLVDGAIVVTEL
                     ADRRMAEGMKRHDAYSSAARRMAWPIIASTCTTLAAFLPLAFWPDMMGEFMKYLPITL
                     IAVLSASLLMALVFVPTLGSLFGASGAFSERARRDLTLAETGNLDEISGLTGSYIGFL
                     RRSLKRPWFNVGAVTGLLVVIYVAFFTLGRGVEFFPAVEPEFATLEIRARGDLSTEEK
                     DLLVSQVETFVLGLEEIESVYTKTGGNDRGSSEDQIGTITLNFIDWDVRRPADQILAE
                     IRGSVSGLAGIDIQTRKPREGPQRGKPIQIELSSKFPDRLETATGRVRAALETIEGVI
                     NIDDTRPLPGIEWQIDVDRAEAARFGADITLVGNM"
BASE COUNT          414 a          686 c          692 g          374 t
ORIGIN      
        1 atcgagaacc gtcgtgctgt cgctggcggt gatcatcgtc tcgggcgtga ttacctacat
       61 cggcgttccc aaggaagcgg agccggatct caagataccg atggtctacg tcagcatgac
      121 gcacgacggg atttcgccgg aggacgccga acgcctcctg gtacggccga tggaacagga
      181 aattcgcacc atcgagggca tcaaggaact gtcgtccaat gcctacgaag gcggcgccag
      241 cgtattgatc gaattcgacg cgggcaccga cgtggacgtc gcgctgcagg acgcccgggc
      301 caaggtggat gccgcgaagg ccgaactgcc gagcgaaacc gaagagccta ccgttacgga
      361 gatcaggttt tctcgtgaac agcccatgct ggtgctgaac ctcgcgggcg acgtgccgga
      421 aagaacgctc aacactatcg ccagggacct caaggagagc atcgagagcc tcccgggggt
      481 catggaggtg acgctcgtcg gcgttcgcga ggaactgatg gaaatcgtcg tcgacccgcg
      541 agccatggaa agctacgggc tcgaccaggc gcagatcctg accctgatcg acaggaacaa
      601 ccggctggtc gccgcaggcg cgctgcatac cagcgaaggc cgttttccgg tcaaggtgcc
      661 gggcgtcttc gaaacggcgg aagacgtgct cgagatgccc atcaaggcgg tcggcgagcg
      721 tgtcgtgcac tttcgcgacg tggccgaggt tcggcggacc tacaaggacg ccgagagcgt
      781 cgcccgccta aacggcgatc gcgcactggc catcgaagtg gtgcaacgca gcggcgccaa
      841 catcatcgag acggttgccg ccgtcaacgc catcgtggag caggcgcggg cagcgtggcc
      901 cgggggcatc caggttttcg cttcgagcga caagtcgaaa gacgtccgcg acatgttgtc
      961 gaacctgcag aacagcgtca tcacggccgt gctcctggtc ttcatcatca tcgtcggcat
     1021 cctgggcatc cgctccgcag gcctggtcgg catctcgatt ccggggtctt tcctgctcgg
     1081 catcctgacg atcgcctcgt tcggcatcac gatcaacatg atggtgctgt tcgcgttgat
     1141 catggccgtc gggctgctgg tcgacggcgc catcgtcgta accgaactcg cggaccggcg
     1201 catggcggag ggcatgaagc gtcacgacgc ctactcgagc gccgcccgca ggatggcctg
     1261 gcccatcatt gcctcgacct gcacgacgct ggcggctttc ctgccgctcg cgttctggcc
     1321 tgacatgatg ggcgaattca tgaaatacct gccgatcaca ctgatcgccg tgctgtccgc
     1381 gtcgctgctc atggccctgg ttttcgtgcc gacgctgggc tccctattcg gcgcatcggg
     1441 cgcgttcagc gagcgggcca ggcgcgacct gacgctggcg gaaaccggca atctcgacga
     1501 gataagcggg ctgaccggct cctatatcgg ctttctccgg cgctcgctca aacgaccctg
     1561 gttcaacgtg ggcgcggtga cgggcctctt ggtcgtcatc tacgttgcct tttttacgct
     1621 gggccgcggc gtcgagttct tcccggccgt cgagcccgag tttgcgacgc ttgaaataag
     1681 ggcgcgcggc gacctgtcga cggaagagaa agatctcctg gtgagccagg tcgaaacatt
     1741 cgtgctcggg ctggaagaga tcgagagcgt ttacacgaaa accggcggca acgaccgcgg
     1801 ttcctcggaa gaccagatcg gcacgatcac cctcaacttc atcgactggg acgtgcgccg
     1861 gccagccgac cagatcctcg cggaaatccg cgggagcgtc tccgggctcg caggcatcga
     1921 catccagacg cgcaagcctc gcgaaggccc gcagcggggc aagccaattc aaatcgaact
     1981 gtcttccaag ttcccggacc gactcgaaac cgcgaccggc cgcgtgcgcg cggcgctcga
     2041 aacgatcgag ggcgtcatca acatcgacga cactcgcccc ctgccgggca tcgaatggca
     2101 gatcgacgtg gaccgcgccg aggcggctcg cttcggcgcg gacatcaccc tcgtcggcaa
     2161 catgat
//
LOCUS       DATDAA010000055         3207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_109064,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000055 DATDAA010000000
VERSION     DATDAA010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109064"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1877
                     /locus_tag="VIS73_01880"
     CDS             165..1877
                     /locus_tag="VIS73_01880"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEY5761938.1"
                     /translation="MSEKHPKDVSADGIERSGWCGVVARNPRKTVISVIVVGLLFGIS
                     FWGALNTFLEYTNRMEFCLSCHEMTIPYEEYKKTIHYSNRTGTTVTCADCHVPSSKTP
                     GDYTRKVVRKIEAVRDIWGHLVGTIDTPEKYEEHRLTMAEREWARMKKADSQECRNCH
                     KFETMRTDKQKKRAVRKHEEAIEEKMTCIDCHKGIAHKAVHIQREEVEPEPEEIAEVK
                     ATVVAEAAAPTPAASAEPAAPAEAPKAAVVPAAVVGPVDWTKVPTKVVPLFYPGQAGL
                     EWVWHKPDHSSANQLLEKGRACTYCHDEDAQDIGKLIAAGKPAGAAKAILDSEIPPGK
                     RGAIPVTVYAAHDATKLYLRFEWVASKGKPVTKMDPKNEVKVAIMIDDNKVEGAKSNG
                     CWSTCHMDLRTMPDADDNAKNHSKAKALGWQDGVTKYIKESRGDLSLEAKPRGAWDKL
                     KSDADIATALGAGQFMDLMQFRSGKGEKIVDGYVLDSRVMDGGKGLIKAEGRKEGNKW
                     VVTMERLLEPDGKGDHKIVGGRQYNIGFAIHEDFANARFHHVSLGYTLALDDEDAFFN
                     VVKQ"
     gene            1993..2571
                     /locus_tag="VIS73_01885"
     CDS             1993..2571
                     /locus_tag="VIS73_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5761939.1"
                     /translation="MTIRSKWLIGLGIVAASATVSAAPPSAEMLSYACAGCHGTNGVS
                     AGPSMPSLAGQNKEAFVAAMKAFRDGDRPSSIMGRLAKGYTDEQVETLATFFAKQKHV
                     PATGQKLDPKLVKSGKALYSKHCKRCHLEGGKEFEEDQPVVAGQWLTYLLIQMDEFKA
                     GNRKMSEKKAEKIDPLKLEELQAIAHYYASVK"
     gene            2584..>3207
                     /locus_tag="VIS73_01890"
     CDS             2584..>3207
                     /locus_tag="VIS73_01890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEY5761940.1"
                     /translation="MTIDRRSFIKLLSAGAGFGAVGVPFAATANELMPKKGRRVVVIG
                     GGYGGTIAAKYIRMNDPSIEVVLVERNTKFVSCPFSNLVIGGNRQMSDITIGYDKLGK
                     NHGIQIVHAEVTDIDAAGKKVVTSKGTLSYDRLVVSPGIDFRTEEIEGYTDDTPNRVP
                     HAWKAGDQTVLLRKQLTDMKDGGTVVLSIPLSPFRCPPGPYERTSQIA"
BASE COUNT          703 a          942 c         1039 g          523 t
ORIGIN      
        1 ccagggcccc aaagggttat agacagtcgg ccggcgcaaa aagaaactcg ggttttctgg
       61 cgccgggcat caacgcgcgc cccggaaacc ctgttgcgac gcagcattca gacgattggc
      121 aattgcccga ggcgcacaag acgccgggtc gaaagggagg gttggtgagc gagaagcacc
      181 ccaaggacgt ttctgcggac ggtattgaaa gaagcggttg gtgcggcgtc gtcgcccgca
      241 atccgcgcaa gactgtcatc tcggtcatcg tcgttggcct gctgttcggc atctcgttct
      301 ggggcgccct taacaccttt ctcgaataca ccaaccggat ggagttctgt ctctcgtgcc
      361 acgagatgac cattccctac gaggaataca agaagaccat ccattattcg aaccgcaccg
      421 gcacaacggt gacttgtgcc gattgccacg ttccgagcag caagacccct ggtgactaca
      481 cgcgcaaggt agtgcgcaag atcgaggcgg tgcgcgatat ctggggccat ctcgtcggca
      541 cgattgatac gcccgagaag tacgaggagc accggttgac gatggccgag cgcgaatggg
      601 cgcgcatgaa gaaggccgac tcgcaggagt gccggaactg ccacaagttc gagacgatgc
      661 gcacggacaa gcagaagaag cgggctgtcc gcaagcacga ggaggccatc gaagagaaga
      721 tgacctgcat cgactgccac aagggcatcg cgcacaaggc cgtgcacatc cagcgcgagg
      781 aagtggagcc tgagcctgaa gagatcgccg aggtcaaggc cacggttgtc gccgaggcgg
      841 cggctccgac tccggccgcc agcgccgaac cggcggctcc agcggaagcg cccaaggctg
      901 ccgtcgtccc ggccgcggtt gtcggtcccg tcgactggac caaggtgccg accaaggtgg
      961 tgccgttgtt ctatccgggc caggcaggtc tggagtgggt ctggcacaag cccgatcatt
     1021 cctcggccaa ccagctgctc gagaagggtc gtgcctgcac ctactgccac gacgaggacg
     1081 cccaggacat cggcaagctg atcgccgccg gcaagccggc cggggcagcc aaggccatcc
     1141 tcgacagcga gattccgccg ggcaagcgtg gggcgatccc ggttaccgtc tacgccgcgc
     1201 acgacgcgac gaagctttac ctgcgcttcg agtgggtcgc cagcaagggg aaacccgtga
     1261 cgaagatgga cccgaagaac gaggtcaagg tggcgatcat gatcgacgac aacaaggtcg
     1321 agggtgccaa gtcgaacggc tgctggtcaa cctgccacat ggatctgcgg accatgcccg
     1381 atgccgacga caacgcaaaa aatcactcga aggcgaaggc gcttggctgg caggacgggg
     1441 tgaccaaata catcaaggag tcgcgcggcg atctgtccct cgaagccaag ccgcgcggtg
     1501 cctgggacaa gctcaagtcg gatgccgaca tcgccactgc gctcggcgcc ggccagttca
     1561 tggacctcat gcagttccgc agcggcaagg gcgaaaagat cgttgatggc tacgtgctcg
     1621 attcgcgggt catggatggc ggcaaggggc tgatcaaggc cgagggccgc aaggaaggca
     1681 acaagtgggt cgtcaccatg gagcgcctgc ttgaacccga cggcaagggc gatcacaaga
     1741 tcgtcggcgg gcggcaatac aacatcggct tcgcgattca cgaggatttt gccaacgcga
     1801 gattccatca tgtgtcattg ggatatacgc tggcgcttga cgacgaggat gcgttcttca
     1861 acgtcgtcaa acaataagaa cagagaacgc aagagacagc aggttggtgt cggtcgattc
     1921 cggatttctc ggtgatcggg cggcgacagg gtgccggtaa gtcgccggtg gtcaatagtg
     1981 gaggagggaa gaatgacaat ccgaagcaag tggttaatcg ggctcggcat cgttgcggcg
     2041 tccgctacgg tttcggcggc accaccgtcg gccgagatgc tgtcttacgc gtgcgccggc
     2101 tgtcacggca ccaatggcgt gtcggcaggt ccgtcgatgc cgtcgctggc cgggcagaac
     2161 aaggaagcgt tcgtcgcggc gatgaaggcg ttccgggacg gcgaccggcc gtcgtcgatc
     2221 atgggccggc tcgccaaggg ctataccgat gaacaggtcg agacgctggc aacgtttttc
     2281 gccaagcaga agcatgtgcc ggccacgggc cagaagcttg atccgaagct ggtgaagtcg
     2341 ggcaaggcgc tctacagcaa gcactgcaag cgttgccacc tcgaaggcgg caaggagttc
     2401 gaggaggatc aacccgtcgt cgccggccag tggctcacct atctgctgat ccagatggat
     2461 gagttcaagg cgggcaatcg caagatgtcc gaaaagaagg cggagaagat cgatccactg
     2521 aagctcgaag agctgcaggc gatcgcccat tactacgcca gcgtcaaata agggaggggc
     2581 atcatgacca tcgatcgtag aagtttcatc aagctccttt ctgccggtgc aggtttcggt
     2641 gccgttggcg ttccgttcgc ggccactgcc aacgagctga tgccgaagaa gggtcgccgt
     2701 gtcgtggtga tcggcggcgg ctacggtggc accatcgccg ccaagtacat ccgcatgaac
     2761 gacccgagca tcgaagtcgt cctcgtcgag cgcaacacga agttcgtctc ctgcccgttc
     2821 agcaacctgg tgatcggcgg caaccgccag atgtccgaca tcaccatcgg ctacgacaag
     2881 ctcggcaaga accacggcat ccagatcgtc catgccgaag tgaccgatat cgacgccgcc
     2941 ggcaagaagg tcgtcaccag caagggaacg ctgagctatg accgtctcgt cgtttcgccg
     3001 ggtatcgatt tccgcaccga ggagatcgaa ggctacaccg acgacacccc gaaccgggtt
     3061 ccgcacgcgt ggaaggccgg cgatcagacg gtgctgttgc gcaagcaact gaccgacatg
     3121 aaggatggcg ggactgtcgt tctgtcgatt ccgctgtcgc cgttccgctg cccgcccgga
     3181 ccctacgagc ggaccagcca gatcgcc
//
LOCUS       DATDAA010000056         4174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_388381,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000056 DATDAA010000000
VERSION     DATDAA010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388381"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VIS73_01895"
     CDS             complement(<1..140)
                     /locus_tag="VIS73_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY5761941.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITSILSKKFGGEA
                     KD"
     gene            complement(206..280)
                     /locus_tag="VIS73_01900"
     tRNA            complement(206..280)
                     /locus_tag="VIS73_01900"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Thr,seq:ggt)
     gene            complement(342..415)
                     /locus_tag="VIS73_01905"
     tRNA            complement(342..415)
                     /locus_tag="VIS73_01905"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(381..383),aa:Gly,seq:tcc)
     gene            complement(494..578)
                     /locus_tag="VIS73_01910"
     tRNA            complement(494..578)
                     /locus_tag="VIS73_01910"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Tyr,seq:gta)
     gene            695..2089
                     /locus_tag="VIS73_01915"
     CDS             695..2089
                     /locus_tag="VIS73_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5761942.1"
                     /translation="MKPIDTPRKPLPETLREALDTRFGKRFSQGESICLQHGRDESAL
                     PPMPPDAVVFAENTEEVSWLAAQCHQHRVPLIAYGTGSSLEGHLLAVRGGISVDLAAM
                     NRVLAIRPDDLDATVQPGVTRKMLNEALKDSGLFFPIDPGADASLGGMAATRASGTNA
                     VRYGTMRENVLALEVVLADGQIVRTGSRARKSSAGYDLTRLFVGSEGTLGIITELTLR
                     LSPVPEAIASATCAFPDVDRAVRTVIETIQLGVPIARIELLDALSIHAINRHSRLALP
                     EQPTLFFEFHGSPHGVKEQVERVEALAGDNGGQSFVWATRTEDRSRLWQARHDAYFAC
                     LQLAPGARSLTTDVCVPISQLAECIAATRSDIEASGLQVPLLGHVGDGNFHLLVLVHP
                     DDTDENARAAELASRVVERAIALGGTCTGEHGVGIGKRQYLPIEHGDPAVALMRRIKY
                     ALDPADILNPGKIV"
     gene            2143..>4174
                     /locus_tag="VIS73_01920"
     CDS             2143..>4174
                     /locus_tag="VIS73_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY5761943.1"
                     /translation="MRPIDFSLDDKYTTIGGRVFMTGVQALVRLPMLQRLRDQATGLD
                     TAGFVSGYRGSPLGALDLQLQQASEHLARHQIVFQPGVNEDLAATAVWGSQQVGLFPG
                     ARHQGVFALWYGKGPGVDRSGDVLRHGNAAGSSRHGGVLVVAGDDHAAKSSTLPHQTE
                     HIFKAVLIPVLAPANVQEYLDFGLHGWAMSRYSGCWVAFKALADTVETSASVLVDPLA
                     LDIRLPTDFSLPEGGLNIRWPDPPLAQEARLLNYKLYAALAYCRSNRLNRVVIDSPNP
                     RLGIISAGKSYLDVRQAFDELGIDDALAAKIGIRLYKVGMVWPLEADGVRHFAQGLEE
                     ILVVEEKRQMLEYQLKEELYNWREDVRPRVIGKFDDKGEWALLPDGRGHLVHGDWLLP
                     AAGELSVAQIARVIAARIDRFFTSETIRERLQLIEAKQRALLQPLTLATRTPYFCAGC
                     PHNTSTRVPEGSRAVAGIGCHYMVTWMDRATATFTHMGGEGVPWVGQAPFTDERHIFA
                     NLGDGTYFHSGILAIRQSVAAGVNITYKLLFNDAVAMTGGQPVDGKLTVPRLTRQLEA
                     EGVSRQVIVTDEPEKYAAIHGLAPDVPVVHRDALDRIQRELRDTPGTTILIYDQVCAT
                     EKRRRRKRGRLVDPALRVVINKEVCEGCGDCSQQSNCLAIVPVDTEFGRTRAI"
BASE COUNT          782 a         1443 c         1256 g          693 t
ORIGIN      
        1 tagtccttcg cctcgccacc aaacttcttc gacagaatcg acgtgatcgc cgccgtcaac
       61 gtggtcttgc catggtcaac gtgcccaatc gtccccacgt tcacgtgcgg cttcgtacgc
      121 tcaaactttc ccttggccat tattccgttc ctcaatcatc gaaaacgcta aaaagtccca
      181 cttgccggct ggtggcaccg taacgtggtg cccatgggca ggattgaact gccgacctct
      241 cccttaccaa gggagtgctc taccactgag ctacatgggc actgcaacag tcaaacacca
      301 gcaaaaaacc taattcccat cgttgccacc aaatctgcct gtggagcggg tgaaggggat
      361 cgaaccctcg tcttaagctt ggaaggcttc tgctctacca ttgagctaca cccgcctgac
      421 ccacgacatc cactacgcgc cggcgcggaa accgcgcagg ccaaacaaca gtcatacgat
      481 gcaacaagca atctggtgga gggggaagga ttcgaacctt cgaaggcaga gccgtcagat
      541 ttacagtctg atccctttga ccgctcggga acccctccga tagaaacgcg gcattatggt
      601 gacaaagttc tggcgtgtca aaccttttgc tgctaactga agtgctcgct aacgtcgtcg
      661 gcgtttcgcc ggaaacgccc gccaaggaag caaaatgaag ccaatcgaca caccgagaaa
      721 accccttccg gagacgctgc gcgaggcact cgatacccgc tttggcaagc gcttttcaca
      781 gggcgaatcg atctgcctgc agcacgggcg cgacgaatcg gcgctgccac cgatgccgcc
      841 tgacgcggtg gttttcgcgg agaacaccga ggaggtgtcc tggttggctg cgcagtgcca
      901 ccaacaccgg gtaccactga tcgcctacgg caccgggtcc tcgctcgaag gccacctgct
      961 ggcggttcgc ggcggcattt cagtcgatct ggcagcgatg aaccgggtac tggccatccg
     1021 ccccgacgat ctcgatgcaa cggttcagcc cggggtcacg cgcaagatgc tgaacgaggc
     1081 gctcaaggac agtggccttt tcttcccgat cgaccctggt gccgacgctt cgcttggcgg
     1141 catggcggca acgcgcgcct cgggcaccaa cgcggtgcgc tacggcacca tgcgcgagaa
     1201 cgtgctggcg ctcgaagtcg tactggccga cgggcagatc gtccgcaccg gcagccgcgc
     1261 gcgcaaatcc tcggccgggt acgacctgac ccgcctgttc gtcggctcgg aaggcacgct
     1321 gggcatcatc accgaactga ccttgcgcct ctcgccggtt cccgaggcga tcgccagcgc
     1381 cacctgcgcc ttccccgacg ttgaccgcgc cgtccgtacc gtcatcgaga ccatccagct
     1441 cggcgtaccg atcgcccgca tcgaactgct cgacgcgctg tcgatccacg ccatcaaccg
     1501 ccatagccgc ctggcgctgc ccgagcaacc gacgctcttc ttcgaattcc acggcagccc
     1561 gcatggcgtc aaagagcagg tcgagcgggt cgaggcgctg gccggtgaca acggtgggca
     1621 atccttcgtc tgggcaaccc ggaccgagga tagatcgcgg ctctggcagg ctcgccacga
     1681 cgcctatttc gcctgcctgc agctggcgcc cggcgcccgc tcgctgacca ccgacgtctg
     1741 cgtaccgatt tcgcagctgg ccgaatgcat tgccgcgacg cgcagcgaca tcgaggccag
     1801 cggactgcag gtgccgctgc tcggccacgt cggcgacggc aactttcacc tgctggtact
     1861 cgtccatccc gacgacaccg acgaaaacgc ccgtgccgcc gaactggcga gccgcgtcgt
     1921 cgagcgggcg atcgcgctcg gaggcacctg caccggcgag catggcgtcg gcatcggcaa
     1981 gcgtcagtat ctgccgatcg aacatggcga cccggcggtc gcactgatgc gccggatcaa
     2041 gtacgcgctc gatccggcgg acattctgaa ccccggcaag atcgtgtagt ccgacgcatt
     2101 gaccggccac cgcaacgggg ccggcaagac ggagcgaagc gcatgcggcc gatcgacttc
     2161 tcgcttgacg acaaatacac gacgatcggt ggccgcgtct tcatgaccgg cgtacaggcg
     2221 ctggtccgcc tgccgatgct acaaaggctg cgcgatcagg ccaccggact cgatacggcg
     2281 ggattcgtct ccggctaccg cggcagcccg ctcggcgcac tcgatttgca gctgcagcag
     2341 gcaagcgagc atctcgcacg ccaccagatc gtcttccagc ccggcgtcaa cgaagacctg
     2401 gcggcgacgg ccgtctgggg ctcacagcag gtcggcctgt tccctggggc gcgccatcag
     2461 ggcgtatttg cactctggta tggcaagggg cccggcgtcg accgcagcgg cgacgtcctc
     2521 cgccacggca atgcggccgg ctcctcgcgc catggcggcg tgctggtggt cgccggcgac
     2581 gaccacgctg ccaagtcatc gacgctgccg caccagaccg aacacatctt caaagcggtc
     2641 ctgattccgg tgctggcacc ggccaatgtt caggagtacc tcgacttcgg cctgcacggc
     2701 tgggcgatga gtcgctattc gggctgctgg gtcgcgttca aggcactggc cgacacggtc
     2761 gagacctcgg cctcggtgct cgtcgatccg ctggcgctcg acatccgcct gccaaccgac
     2821 ttcagcctgc ccgagggcgg cctcaacatc cgctggcccg acccgccgct ggcccaggag
     2881 gcgcggctgc tcaactacaa gctctacgca gcgctcgcct actgccgcag caaccgcctc
     2941 aaccgcgtgg tgatcgacag ccccaatccg cggctcggca tcatcagcgc cggcaagtcc
     3001 tacctcgatg tccgccaggc cttcgacgaa ctcggcatcg acgatgcgct ggcagcgaaa
     3061 atcggcatcc gactctacaa ggtcggcatg gtctggccgc tcgaggccga cggggtccgc
     3121 cacttcgccc aaggactaga ggaaatcctc gtcgttgagg aaaagcggca gatgctcgaa
     3181 taccagctca aggaagagct ctacaactgg cgcgaggacg tccgtccgcg ggtcatcggc
     3241 aagttcgacg acaagggtga gtgggccctg ctccccgacg gccgcggcca cctcgtccac
     3301 ggggactggc tgctgccggc cgccggcgag ctgtcggtgg cgcagatcgc ccgcgtcatc
     3361 gccgcacgga tagaccgttt ctttacctcc gagactatcc gcgagcgcct gcaactgatc
     3421 gaagccaagc agcgcgcgtt gctgcagccg ctgacgctgg cgacacgaac gccctacttc
     3481 tgcgccggct gcccgcacaa cacctcgacg cgcgttcccg aaggttcgcg cgcggttgcc
     3541 ggcatcggct gccactacat ggtcacctgg atggaccggg cgacggcgac cttcacccac
     3601 atgggcggcg agggagtgcc ctgggtcgga caggcgccgt tcaccgacga gcgacacatc
     3661 tttgccaacc tcggcgacgg cacctatttc cactcgggaa tcctggcgat tcgccaaagc
     3721 gtcgccgccg gcgtcaacat cacctacaag ctgctcttca acgacgccgt cgcgatgacc
     3781 ggtggccagc ccgtcgacgg caagctgacc gtacccaggc tgactcgcca gctcgaggcc
     3841 gaaggcgttt cccggcaggt gatcgttacg gacgaaccgg aaaaatacgc cgccattcat
     3901 ggtctggcgc ccgacgttcc ggtcgtgcat cgcgatgcgc ttgatcgcat tcagcgcgaa
     3961 ctgcgcgaca cgccgggcac gacgatcctg atctacgacc aagtctgcgc caccgagaaa
     4021 cggcggcgcc ggaaacgcgg ccgcctagtc gatccggcgc tgcgcgtggt catcaacaag
     4081 gaggtatgcg aaggctgcgg cgactgttcg cagcagtcga actgcctggc gatcgtgccc
     4141 gtcgacaccg aattcggccg cacgcgggca attg
//
LOCUS       DATDAA010000057         3318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_213958,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000057 DATDAA010000000
VERSION     DATDAA010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213958"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            361..2529
                     /locus_tag="VIS73_01925"
     CDS             361..2529
                     /locus_tag="VIS73_01925"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY5761944.1"
                     /translation="MRPLGSAQPHDLPDPLARAIETAKVPALPQVLLKLLQLTDDDAA
                     TMSEIATVVERDPGLCTRLLTAANSPGLRRGTELRSIAHCLTSLGTRLIRSIATCLAL
                     QSMFDRRYEAVRPDLAEYWRHSIRVAETARELATVCGYPQPDEAYLGGLLHDIGELVL
                     LSAQGSTYAQLLSSARNEAKLVALENEHLGAHHGEVGAWVVDRWDFDLDIADSILFHH
                     AKADEINTADQLPQVVWLAHALATAAPTDPAVRQHADRLFGETIGEQIETIGRRTLER
                     TAQIAEAIGIVADPSPDGVPLTVLPQVAVNTPRPPETRGEGEVDQFVRDMALMQPLQR
                     DLFGVESDTELLLSLRESARILFDLGHIAIFLRGADDDRLSAARFGVQPPLLRRAAIR
                     VDATNCLVATAAAERRIASSFDEDYPQPASLIDVQFARVFGTDGLLCVPLNGRGRTIG
                     VMVFGLSAAQHGRLQRRLPWVLNFGRIAALSIEVWHEAIRRRKQADDDASAHFERQAR
                     RVIHEAGNPLGIIRSYLQILDGKLPEDASAREEIVVLREEIDRVSTIVRRLSDIPRGD
                     SGDRLLNVGSVVRELLVFYDEALFKSRGIEVAFDPGAGGSDPVACDRDSLKQILINLW
                     KNAAEAMGSGGRLEVSIDDRVVQNGRTYVGISISDNGPGMPAEALARLADAERGGGPG
                     ERGNGLAIVSALVAQLGATLNCRTRTDSGTTFELLLPCCE"
     gene            2597..>3318
                     /locus_tag="VIS73_01930"
     CDS             2597..>3318
                     /locus_tag="VIS73_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5761945.1"
                     /translation="MEVIPGFGMDMRTLVAESTAQILIVDDEPRIRAAYRKLLAADGR
                     HIEECENGRDAIARLDRQDVDVLILDLGLPDINGREVMEWVARNQLSTSIIVFSAEDA
                     IDAAILALRRGACEFIRKNSDPQDLVRTVDQVLRRRRLEREHALMTAQLEQSERLHRF
                     LVENSPDIIYTLDSDGCFVFINSRVESLLGYDNGELIGQHYSKIVHPDDRESARYAFA
                     ERRVGNRATFNFEIRLCGKDHSV"
BASE COUNT          576 a         1135 c         1049 g          558 t
ORIGIN      
        1 tggcctgcgc ttcgggccgc ctcaccccgg gaacgatggt gcggatcgtc gacagcgagg
       61 aaggcggcgc gctgaagatc ggcgcaccga tcgagcgcaa cgggcgcttc gagttgctgc
      121 tgctcgatcc gcagccaggc acctaatact taagtcatat ccactcagta aaggtcgcga
      181 caaagtgtcg cgcccccgcc ttgaattaaa cgggcttaat gggttgcgac aagtgcaaaa
      241 cttgtccata ttctcgtgga gttccgcggc ccgtccggcc gcggcacatt tgtttagcat
      301 ctgccgcacg gcgcagccca gtcttccggg tcgtctatgc ggcccgcccc ggagtcgccg
      361 atgaggccgc ttggaagtgc acaaccgcat gacttgcccg acccgctggc gcgcgccatc
      421 gagacggcca aggtgccggc acttccgcaa gttctgctca aactcctgca gctgaccgat
      481 gacgacgcgg caacgatgtc ggaaatcgcc accgtcgtcg agcgcgatcc gggcttgtgc
      541 acgcgcctgc tgaccgcagc caactcgcct ggactgcgcc gaggcacgga attgcgcagc
      601 atcgcacact gcctcacgtc gctgggcacg cgactaatcc gctcgatcgc cacctgcctc
      661 gcgctgcagt cgatgttcga tcgccgctac gaagcggttc gccccgatct ggccgagtac
      721 tggcgccact cgatccgcgt cgccgaaacc gcacgcgaac tggcgacggt ctgcggctat
      781 ccgcaacccg acgaggccta cctcggtggc ctgctgcacg acatcggaga gctcgtcctg
      841 ctctcggcgc agggcagcac ctacgcccag ctgctgtcct cggcacgcaa cgaggccaag
      901 ctggtcgcgc tcgaaaacga gcaccttggc gcgcaccacg gcgaagtcgg cgcctgggtc
      961 gtcgaccgct gggatttcga cctcgacatc gccgacagca tcctcttcca ccacgccaag
     1021 gcagacgaga tcaacaccgc cgatcagctg ccgcaggtgg tctggctggc acacgcgctg
     1081 gcgaccgccg cgcccactga tccggctgtt cggcagcatg ccgaccggct atttggcgag
     1141 acgatcggcg aacagatcga gacgataggc cggcgcacgc tcgagcgcac ggcgcagatc
     1201 gctgaggcga tcggcatagt cgccgacccc tcgcccgacg gcgtgccgct caccgtgctg
     1261 ccgcaggtcg cggtcaatac gccgcggcca ccggagacca ggggcgaggg cgaggtcgac
     1321 cagttcgtcc gcgacatggc gctgatgcag ccgctgcagc gcgacctctt cggcgtcgag
     1381 agcgacaccg agctgctgct atcgctgcgc gagtcggcac gcatcctgtt cgacctcggc
     1441 cacatcgcga tctttctccg tggcgccgac gacgatcgcc tgtcggccgc gcgcttcggt
     1501 gtccagccgc cgctgctgcg ccgcgcagcg atccgcgtcg acgcgaccaa ctgcctggtg
     1561 gcaaccgccg ctgccgagcg gcgcatcgct tcctcgttcg acgaggacta cccgcagccg
     1621 gcctcgctga tcgatgtgca gttcgcccgc gtctttggca ccgacggcct gctctgcgta
     1681 ccactcaacg ggcgcgggag aaccatcggc gtcatggttt tcggtctcag cgctgcccag
     1741 catggccgcc tgcagcgacg cctgccctgg gtcctcaatt tcggccgcat cgcagcactg
     1801 agcatcgagg tctggcacga ggcgatccgc cgccgcaagc aggccgatga cgacgcctcg
     1861 gcccacttcg aacgacaggc gcggcgcgtc attcatgagg ccggcaaccc gctcggcatc
     1921 atccgcagct atttgcagat actcgacggc aagctgcccg aggatgcctc ggcacgcgag
     1981 gagatcgtcg tgctgcgcga ggaaatcgac cgcgtgtcga cgatcgtccg ccggctctcc
     2041 gacattccgc gcggggacag cggcgaccgc ctgctcaacg ttggctcggt ggttcgcgag
     2101 ctcttggtct tctacgacga agccctgttc aagtcgcgcg gcatcgaggt cgcgttcgat
     2161 cctggtgccg gcggcagcga cccggttgcc tgcgaccgcg acagcctgaa gcagatcctg
     2221 atcaacctct ggaagaacgc tgccgaggcg atgggatcgg gcgggcggct cgaggtatcg
     2281 atcgatgacc gcgtggtgca gaacggcagg acctacgtcg gcatttcgat cagcgacaac
     2341 ggtcccggca tgccggccga ggcgctggcc cggctggccg atgccgagcg cggaggcggg
     2401 cctggagaac gcggcaacgg gctggcgatc gtcagcgcgc tggttgccca gcttggcgca
     2461 acgctgaact gccgcacgcg caccgattcg ggcacgacct tcgaactgct gctgccatgc
     2521 tgcgaatgaa tctgcctgcg gccgcggatg gccggcgtaa gatatgcacg gcagtttgac
     2581 cacgggaggg gtgagagtgg aggtaatccc gggatttggc atggatatgc gcacgctggt
     2641 tgcggaatcc acggcgcaga tcctcatcgt tgacgacgag ccccgaattc gcgctgccta
     2701 tcgcaagcta cttgctgccg acggtcgcca catcgaggaa tgcgagaacg gacgcgacgc
     2761 gatcgcccgc ctcgaccgcc aggacgtcga cgtcctgatc ctcgacctcg gccttcccga
     2821 catcaatggc cgcgaggtca tggagtgggt agcccgcaac cagttgtcga catcgattat
     2881 cgtctttagc gcagaggacg cgatcgacgc ggcgatcctc gcgctgcgac gcggcgcctg
     2941 cgaattcatc cgcaagaatt ccgatccgca ggatctcgta cgtaccgtcg accaggtgct
     3001 gcgccggcgc cggcttgaac gtgaacacgc gctgatgacg gcgcagctcg agcagtcgga
     3061 gcgactgcac cgctttctcg tcgagaattc gcccgacatc atctacacgc tcgactcaga
     3121 cggctgtttc gtcttcatca acagtcgcgt cgagtcgctg cttggctacg acaacggcga
     3181 gttgatcggc cagcactact cgaagatcgt ccaccctgat gaccgcgaga gtgctcgcta
     3241 cgcctttgcc gagcgccgcg tcggcaaccg ggcaaccttc aatttcgaga tccggctttg
     3301 cggcaaggac cactcggt
//
LOCUS       DATDAA010000058         2027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_283872,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000058 DATDAA010000000
VERSION     DATDAA010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283872"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VIS73_01935"
     CDS             <1..230
                     /locus_tag="VIS73_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761946.1"
                     /translation="NRLEAFGEPQLPTLLVGRQAMRGFQADDWNDTLNLAGYPQAAPI
                     GYRAPPPPPTPDERKEVEAPPPEPEDLRANY"
     gene            241..1047
                     /gene="xth"
                     /locus_tag="VIS73_01940"
     CDS             241..1047
                     /gene="xth"
                     /locus_tag="VIS73_01940"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY5761947.1"
                     /translation="MKLATWNVNSLKVRLPHLFTWLEQSPVDVLCLQETKLEDKSFPR
                     AEIEAAGFHVAYSGQKTYNGVAIVSRFPLADVQAGNPHFADEQKRLIAVTVTAPDQAP
                     VRVISAYVPNGQAVGSDKYEYKLAWLAGLRRWLQDELAAHPQLAIAGDYNIAPDDRDV
                     HDPVAWAGQILCSDDERAAFVALTGLGLADAFRLFDQPERVFSWWDYRMMGFRRNHGL
                     RIDHVLLSEALRARCTACVVDRAPRGWERPSDHAPVIATLADTPATGDIS"
     gene            1044..1724
                     /locus_tag="VIS73_01945"
     CDS             1044..1724
                     /locus_tag="VIS73_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY5761948.1"
                     /translation="MSRDARFAQDLTALYPPLAEIDADLRQRIFDNAQTLTVAGGTVL
                     FDEHQPCQGFPFVVKGSVRVVKAGANGRELPLYRVLPGETCVISSSCLLGRIDYNARG
                     VADEETTVVLLPRPLFDELVGAPAFREYVFRLLAERIADLMQLIEEVAFRRLDQRLAA
                     LLLGKGRMLHTTHQHLADELGSVREIVSRLLKGFSEQGFVSLGREQIEILDPAGLRNL
                     AQAGKPGH"
     gene            1840..>2027
                     /locus_tag="VIS73_01950"
     CDS             1840..>2027
                     /locus_tag="VIS73_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEY5761949.1"
                     /translation="MKANVGGIDKILRILAGIGLIAWAVMGGPVWAWIGIVPLATGAI
                     GWCPVYPILGLSTCPAKKA"
BASE COUNT          343 a          730 c          610 g          344 t
ORIGIN      
        1 gcaatcgcct cgaagcgttc ggcgagccgc aactgccgac gctgctggtc ggtcgtcagg
       61 cgatgcgcgg ctttcaggcc gacgactgga acgacacgct caacctggcc ggctatccac
      121 aggccgcccc gatcggctat cgcgcaccgc ctccgcctcc gacgccagat gaacgcaaag
      181 aggtcgaagc gccgcccccc gagcctgaag acctgcgcgc caactactga tgcgggcgca
      241 atgaagctcg ccacctggaa cgtcaattcg ctcaaggttc gcctgccgca tctgttcacc
      301 tggcttgagc agtcgccggt tgatgtgctc tgcctgcagg aaaccaagct cgaagacaag
      361 tccttcccgc gcgccgagat cgaggccgcc ggctttcacg ttgcctacag cggccagaag
      421 acctacaacg gcgtcgccat cgtcagccgc tttccgctcg ccgacgtgca ggcaggcaat
      481 ccgcatttcg ccgacgaaca gaagcgcctg atcgccgtca ccgtcacggc acccgatcag
      541 gcgccggtgc gcgtcatcag cgcctatgtc cccaacggtc aggcggtcgg cagcgacaag
      601 tacgaataca agctggcctg gctcgccggg cttcgccgct ggctgcagga tgaactcgcg
      661 gcgcacccgc agctggcgat tgccggcgac tacaacatcg cccctgacga ccgcgacgtg
      721 catgaccctg ttgcctgggc cgggcagatc ctctgctccg acgacgagcg cgccgccttc
      781 gtggcgctga ccgggctggg cctcgccgac gcctttcgcc ttttcgacca gcccgagcgg
      841 gtgttttcct ggtgggacta ccggatgatg ggattccgcc gcaaccatgg cctgcgcatt
      901 gaccacgtgc tgctttccga agcgctgcgc gcgcgctgca cggcctgcgt cgtcgatcgc
      961 gcgccgcgcg gctgggaacg cccctcggat cacgcgccgg tgatcgccac gctggccgac
     1021 acgccggcaa ccggagacat ctcgtgagcc gtgacgcccg ttttgcccaa gatctcaccg
     1081 cactctatcc gccgttagcc gaaatagacg ccgacctgcg ccagcgaatc ttcgacaacg
     1141 cgcagacgct caccgtcgcc ggcggtaccg tgctcttcga cgaacaccag ccctgccaag
     1201 gatttccctt cgtcgtcaaa ggcagcgtgc gagtggtcaa ggccggcgcc aacggccgtg
     1261 aactgccgct ttaccgcgtg ctgccaggcg aaacctgcgt catcagctcg agctgcctgc
     1321 tcggccgcat cgactacaac gcacgcggcg tcgccgacga agaaaccact gtcgtcttgc
     1381 tgccgcgccc gctcttcgac gaactggtcg gcgcgccggc ctttcgcgag tacgtattcc
     1441 gcctgctcgc cgaacgcatc gccgatctga tgcagctgat cgaggaagtc gccttccggc
     1501 gcctcgacca gcgcctcgcc gcgctgctgc tcggcaaggg caggatgctg cacaccacgc
     1561 accagcacct cgccgacgaa ctcggcagcg tgcgcgaaat cgtcagccgg ctgctcaagg
     1621 gattttccga gcagggattc gtcagcctcg gccgcgagca gatcgagatc ctcgatcctg
     1681 cgggcctgcg caatctcgcg caggccggga agccgggtca ctgattttcg gtgattcggg
     1741 tcaccgaccc gcgccggtcg ctcccctagt cttcgcgcca tggcagccgc aatgtctgcg
     1801 tcgctgccgc ccctgcgcaa ttactcaagg agagacttca tgaaagcaaa cgttggtggc
     1861 attgataaga ttcttcgcat cctcgccggc atcggcctga tcgcctgggc cgtcatgggc
     1921 ggcccggtct gggcgtggat cggcatcgtc ccgttggcca ccggcgcaat cggctggtgc
     1981 ccggtctacc cgatcctcgg cctgagcacc tgcccggcga aaaaagc
//
LOCUS       DATDAA010000059         9739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_388878,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000059 DATDAA010000000
VERSION     DATDAA010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9739
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388878"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VIS73_01955"
     CDS             <1..213
                     /locus_tag="VIS73_01955"
                     /inference="COORDINATES: protein motif:HMM:NF021165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761950.1"
                     /translation="VLASLDGAAPMKISEIPYIRRKHLEIPPEQMKGNPKVGSIAQCD
                     ACHTEAAQGNFDDDSVVIPGYGRWRW"
     gene            complement(182..745)
                     /locus_tag="VIS73_01960"
     CDS             complement(182..745)
                     /locus_tag="VIS73_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761951.1"
                     /translation="MNDNAPLALHPQQSLPERLSAWLHQRWALWLFCITVFFVYDLVL
                     DVVAGELDWHIIVEAIVFLICAVVLFTEWRRSVRLGQHLASAQARNLRLSGQFTNYVC
                     ETMASWKLSISEQEIAWLILKGFSFSEIASIRGVQEKTARQQATAVYAKSGCKNRSEF
                     VAHFIQDLLAGDFTPAAITSASGRNPE"
     gene            complement(766..2577)
                     /locus_tag="VIS73_01965"
     CDS             complement(766..2577)
                     /locus_tag="VIS73_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY5761952.1"
                     /translation="MRNYQHLPAPAADTPPPSARRVFLSLFPYLWRYRGRVLAALVCL
                     VLAKVANVGIPLAFKEIIDSFTAEQALLAVPVAMLLLYGALKFSASLFQELREILFAR
                     VTQQTVRHVAIEVFRHLHALSLRFHLERQTGGLSRDIERGSRSISSLISYTLYSILPT
                     LVEISLVLGILFSRYAISFVLITLASLATYVVFTITVSNRRIAIRREVNDADSAANTK
                     AIDSLLNYETVKYFNNERFETSRYDDDLLRWEGAATRSQISLSMLNLGQQLIIALGVT
                     AMMWTAASGVARGEMTIGDLVLVNAFLIQLYAPLNFLGVVYREIRHSLTDIERMFGLI
                     KTHCEIADAPDARPLVVGEGRIEFDAVGFAYDGRRTVLSDVSITVGSGRTIAVVGPSG
                     SGKTTLARLLFRFYDVTSGSIRIDGQDIRQVTQSSLRASIGIVPQDTVLFNDTLFYNI
                     RYGNPEAPPEAVYAAARAAQLDQFVSMLPDGYETRVGERGLKLSGGEKQRVAIARTLL
                     KNPPILMFDEATSALDSRTEKAIQESLDAVSRGRTTLVIAHRLSTIMHADEIVVLENG
                     AITERGTHGELLERGGRYAHMWALQRQSEKAALAETP"
     gene            2689..3387
                     /locus_tag="VIS73_01970"
     CDS             2689..3387
                     /locus_tag="VIS73_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5761953.1"
                     /translation="MSSIPEKRQPAQQPDTRRRLLDAAERVLMENGFEGASMRMITAE
                     AGVNLAAANYHFGSKEGLIEAVFRRRLAWLNEERMRRLDALEAAAAGEPLRPSQILEA
                     FFGTLFQMGEDEELGGMVFLRLVGRTLTEPAAFIRNFFAGAYDDVVRRYKKALVVSLP
                     GVPEEEIAWRFHFMLGAMSYAISGIDSLRVVGGLDVTDEIPDALAAKRLRARLMPFLL
                     GGLRAPLPGTGGTA"
     gene            3411..5753
                     /locus_tag="VIS73_01975"
     CDS             3411..5753
                     /locus_tag="VIS73_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY5761954.1"
                     /translation="MTLLLIIVALCAVVVTVPAIRRPLLSTPIFAAYRRILPQMSETE
                     RAALEAGTVWWEGELFRGRPDWQRLLDYPQPTLSAEEQSFLDNEVEQACALVDDWQVS
                     HELQDMSSEAWQYIKEKGFLGMIIPKHYGGLEFSAYAHSQVVTKLSTRSSALSVSVMV
                     PNSLGPAELLLHYGTDAQKDHYLPRLARGEEVPAFALTSPWAGSDAAAIPDNGVVCRG
                     QWEGEEVLGMRLSFDKRYITLAPVCTVFGLAFHVYDPERLLGGEADIGITCALIPATH
                     PGVEIGRRHFPLNAQFMNGPVRGKDVFVPLDFIIGGPKMAGQGWRMLMECLAAGRSIS
                     LPASNTGMAQLTARAVGGYARVRQQFGMPIGRFEGVEEALTRIGSFTYLMDATRKMTA
                     GAVDLGEKPSVVSAIAKYHVTERARQVVNDGMDVIGGKGICLGPANILGRAYQQIPIG
                     LTVEGANILTRSLIIFGQGAIRCHPYVLAEMKAAQDSNTDRGLEHFDSALWGHVVFSI
                     RNGARALWLGMTGSHFAPVPDGYSEETARYVQQLTRFSSAFAFLSDVSMLVLGGGLKR
                     REKLSARLGDILSQMYLMSATIKRFEAEGRQSADAPLMHWAIWDSMYRAQEAFDGVLA
                     NYPNRVVAWFLKRIIFPWGHPYVVPSDALGHAAARLLIDATPTRERLTAETYVAGDAN
                     DPVAAIEAALRASIEAEPIDTKIRAADRDGRFADNPGATVRDMADVALAAGVIDQAEH
                     AKLKRRNALRDKVIHVDDFPFDLGISVRRPEQGDAQREAA"
     gene            5750..7057
                     /locus_tag="VIS73_01980"
     CDS             5750..7057
                     /locus_tag="VIS73_01980"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY5761955.1"
                     /translation="MSKAPVFVVDGARTPFLKARGAPGQFSASDLAVQAGRALLLRQR
                     FNAEDLDEVILGCAAPAADEVNIGRVCALRMGCGNKVPAWTVMRNCASGMQAIDSALA
                     NIQSGRANLVMAGGVDALSRAPILFSDAMVNWLARWSTARGVGAKLAALRGFRPGYLA
                     PVIGLLKGLTDPVVGLSMGQTAENLAWRFGIDRLAMDAFAAESHLRALAARKAGAFGE
                     IVPLLDRNGVVYAEDDGVREDSTLDRLGKLRPVFDKPYGNVTAGNSSQVTDGAAWLLL
                     ASEAAVTRFGLTPIGRILDSEWAGLDPAEMGLGPVHAATPILQRNGLGLADLDLLEIN
                     EAFAAQVLGCLAAWESAAYCRDGLGLDAPLGKVDRARLNPDGGAIAVGHPVGASGARI
                     VLHLLHALRARGGGRGVAAICIGGGLGGAMLVDTQTTQTGGAA"
     gene            7054..8970
                     /locus_tag="VIS73_01985"
     CDS             7054..8970
                     /locus_tag="VIS73_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY5761956.1"
                     /translation="MSEVEYRHWRIENDSRGVTWLTLDREGETTNALSAAVMAELDCI
                     VSGFERNSPAALIIRSAKEAGFIAGADIREFSALNTTEGAHELLARGWSLFNRIADFR
                     FPTAALIRGHCLGGGLELALACRYRIVVDEPSTRLGLPEVMLGIFPGWGGMRRLPKLI
                     GPQLALDMMLTGKTLDARRAKRSGLADLAVPPRVMVRSAVEHVLSGKAPRRPAMLTRL
                     LNGPLKRVVAKSARKQVATKARPEHYPAPYAIIDCWENHDGDALAIPDYLDAIVSSPT
                     AQNLVRVFHLQERLKGFGKAADFPVKRVHVIGAGVMGGDIAAWCALRGLTVTLQDQSV
                     ERIAPAIKRAATLYSRRLRDRLAERNALDRLIPDPRGDGVGLADVVIEAIFENLEAKH
                     ALFRSIEPRLKPGAVLASNTSSLRLEDLRTVLAQPERLVGIHFFNPVAKMPLVEVVEA
                     EGGDEAEHRKACAFVRTIDKLPLPVKSAPGFLVNAVLAPYMLEAMRAVDEGVAPENID
                     ASMLAFGMPMGPIELADSVGLDIALAAGRELAGGGDAPACLLAHVEAGDLGKKTGKGF
                     YTWVDGKPQKGEARTEPGLADRFVQPLLDRTQALVAERVVADPDLADAGVIFGTGFAP
                     FTGGPMNYLKARNG"
     gene            8963..9352
                     /locus_tag="VIS73_01990"
     CDS             8963..9352
                     /locus_tag="VIS73_01990"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY5761957.1"
                     /translation="MAEEPVMLPPGIPALRVMTMPADANPSGDIFGGWVMAQVDVAGA
                     VPAMRRARGRVATVAVNSFLFKQPISVGDLVSFYAEVVSEGKTSITVNVEVYAERHPA
                     APLIVKVTEAQLTYVALDGKGNKRQLP"
     gene            9419..>9739
                     /locus_tag="VIS73_01995"
     CDS             9419..>9739
                     /locus_tag="VIS73_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEY5761958.1"
                     /translation="MKSMLKVKWISIAVATAFSGSAMAAGFALQNQNGSGNGNAFAGA
                     AAAAEDAGTIFFNPAGMALLPQGKNVAVAGTYLDRSVKFTDAGTSPPTLSTTTSTNGG
                     DAGGS"
BASE COUNT         1732 a         3089 c         3189 g         1729 t
ORIGIN      
        1 gttctggcct cgcttgatgg cgcggcgcca atgaagatca gcgagatccc ctacattcgc
       61 cgcaagcacc tcgaaattcc gccggagcag atgaagggga atccaaaggt aggctcgatt
      121 gcgcaatgcg atgcctgtca caccgaagcc gcacagggca actttgacga cgacagtgtg
      181 gtcattccgg gttacggccg ctggcgctgg tgatcgcggc gggggtgaag tctccggcaa
      241 gcaggtcctg aatgaagtgg gcgacgaatt cgctgcggtt cttgcatcct gatttcgcgt
      301 agaccgcggt agcctgctgc ctcgcggttt tttcctgaac gccgcggatc gatgcaattt
      361 cggaaaacga gaaacccttt aggatcaacc aagcgatttc ctgctcggag attgagagct
      421 tccaggaggc catcgtctcg cacacataat tggtgaactg tccggaaagt ctcaaattgc
      481 gcgcctgcgc cgaggctaga tgctggccga ggcggacgct gcgacgccat tcggtaaaca
      541 gtacgactgc gcagatcaga aagacgattg cctcgacgat gatgtgccag tcaagctcac
      601 cggccacgac atcaagtaca aggtcgtaga caaagaagac cgtgatacag aagagccaga
      661 gtgcccagcg ttgatgcagc caggccgaca gccgttcggg aaggctctgc tgcgggtgca
      721 gcgcaagcgg ggcgttgtcg ttcatgggcg cgatgatccc gtgccttatg gggtttcggc
      781 aagcgccgcc ttttccgatt gccgctgcag ggcccacatg tgcgcgtagc ggccgccgcg
      841 ttcgagcaac tcaccgtggg tgccgcgttc ggtaatcgcg ccgttctcga gcacgacgat
      901 ctcatcagcg tgcatgatcg ttgacaggcg gtgagcgatc accagcgttg tgcgtccgcg
      961 ggaaaccgcg tcaaggcttt cctggatcgc cttctcggtc cgcgagtcga gggccgaggt
     1021 tgcttcgtcg aacataagga tcggcgggtt cttgagcagc gtacgggcaa tcgccacacg
     1081 ctgtttctcg ccacccgaga gcttcaggcc gcgttcgccg acgcgggtct cgtagccgtc
     1141 gggcaacatg gaaacgaact ggtcgagctg cgccgcacgg gctgccgcgt agaccgcttc
     1201 cggcggcgcc tcggggttgc cgtagcggat gttgtagaac aaggtgtcgt tgaacagcac
     1261 cgtgtcctgc ggcacgatgc caatcgacgc gcgcaggctg gactgggtga cctgtcgaat
     1321 gtcctgaccg tcgattcgga tcgaccctga tgtgacgtcg tagaagcgga acagcaggcg
     1381 cgccaaggtg gtcttgcctg aacccgaggg cccgacgacg gcgatcgtgc gtccactgcc
     1441 gacagtgatc gacacgtcgc tgagcacagt tcgccggccg tcgtaggcga aaccgaccgc
     1501 atcgaactcg atccgcccct cgccgacaac cagcggccgc gcgtctggcg cgtcggcgat
     1561 ctcgcagtgg gtcttgatta atccgaacat ccgctcgata tcggtcagcg agtggcgaat
     1621 ctcccgataa acgacgccga ggaagttgag cggcgcgtac agctggatca gaaaggcatt
     1681 gaccagcact aggtcgccaa tcgtcatctc gccgcgggca acgccggatg ccgccgtcca
     1741 catcatcgcg gtgacaccga gcgcgatgat caactgctgc ccgagattga gcatcgacag
     1801 tgaaatctgg ctgcgcgtcg cggcgccttc ccagcgcaac agatcgtcgt cgtagcggga
     1861 ggtctcgaag cgctcgttgt tgaagtactt cacggtttcg tagttgagca agctgtcgat
     1921 tgccttggtg ttggccgccg agtcggcgtc gttgacctcg cgccggattg cgattcgtcg
     1981 attggagacc gtaatggtga acacgacgta ggtggccagc gaagccagcg taatgaggac
     2041 gaagctgatc gcatatcgcg aaaaaagaat gccgagcacc aacgagatct cgaccagcgt
     2101 cggcaggatc gaatagagcg tgtagctgat caggctggaa atcgaccgag aaccgcgttc
     2161 gatgtcgcgc gagagcccgc cggtctgccg ctcaaggtgg aaacgcagcg acagcgcgtg
     2221 caggtgtcgg aatacctcga tcgcgacgtg acggacggtc tgttgggtca cccgcgcaaa
     2281 gagtatttcg cgcaattcct gaaagagcga ggccgagaac ttcagcgcgc catagagcag
     2341 cagcatggcc acgggcacgg ccagcagtgc ctgctcggcg gtaaagctgt cgatgatctc
     2401 cttaaaggcg agcggaatgc cgacgttggc gactttggcc agcaccagac aaacgagcgc
     2461 ggcgagtacc cgcccgcggt agcgccagag atagggaaag agcgaaagga agacgcggcg
     2521 cgcgctcggt ggcggcgtat cggcggcggg ggcaggtagg tgctggtagt tgcgcatggc
     2581 gggtgtccga ttgtaagcgg cgccatcggt tccaattgag gcggacttga caagccgcgg
     2641 ttcgccgtct atggttcaaa cgttcgtttg agtcaggtcc cgccagccat gtcctccatc
     2701 cccgaaaagc gtcaacccgc gcagcagcca gatacccgtc ggcgcctgct cgatgccgcc
     2761 gaacgtgtgc tgatggaaaa cggctttgag ggcgcgtcga tgcggatgat tacggccgag
     2821 gccggcgtta acttggcggc cgcgaactat cacttcggct cgaaggaagg gctgatcgag
     2881 gcggtcttcc gccgtcgcct cgcttggcta aacgaggagc gcatgcgccg cctcgatgcg
     2941 ctggaggcag ccgccgccgg tgagcctctg cgcccctcgc agattctcga agccttcttc
     3001 ggcacgttgt tccagatggg cgaggacgaa gaactgggcg ggatggtctt cctgcgcctg
     3061 gtcggccgga cgctcaccga gccagcggcc ttcatccgca atttcttcgc cggcgcctac
     3121 gacgacgtcg tccgtcgcta caagaaggcg ctggtggttt ctctccccgg cgttcccgag
     3181 gaagaaatcg cttggcgttt tcacttcatg ctcggcgcca tgtcctacgc gatttcgggt
     3241 atcgactcgc tgcgcgtcgt tggcggactc gatgtgaccg acgaaatccc cgatgcactc
     3301 gcggcgaagc ggcttcgcgc acgactgatg ccttttctgc tgggcggttt gcgcgcaccg
     3361 ctgccgggca ccggcggcac cgcctgaacc acgatctagg aaccattgcc atgacccttc
     3421 tactgatcat tgttgccctc tgtgccgtcg tcgtgacggt gccagcgatc cgtcggccat
     3481 tgctgtcgac gccgatcttc gcggcctacc gccgcatcct gccgcagatg tccgaaactg
     3541 aacgggcggc actcgaggca ggcaccgtct ggtgggaagg tgagctgttt cgtggtcgcc
     3601 ccgactggca gcgtctgctc gactatccac aaccaacgtt gagcgccgaa gaacagtcct
     3661 ttctcgacaa cgaggtcgag caggcctgcg cgctcgtcga cgactggcag gtctcgcacg
     3721 aattacagga catgagctcc gaggcctggc agtacatcaa ggaaaaggga ttcctcggga
     3781 tgatcatccc caagcactac ggcgggctcg agttttcggc ctacgcgcac tcgcaggtgg
     3841 tgaccaagct gtcgacgcgc tcgtcggcgc tgtcggtatc ggtgatggtg ccgaactcgc
     3901 tgggccccgc cgagttgctg ttgcactacg gaacggacgc gcagaaggat cactacctgc
     3961 cacgtctggc ccgcggcgag gaagttccgg ccttcgcgtt gaccagcccg tgggcagggt
     4021 cggacgcggc ggcgatacct gataacgggg tggtctgccg gggtcagtgg gaaggggagg
     4081 aagtgcttgg catgcgcctc tccttcgaca agcgctacat cacgctcgcc ccggtctgca
     4141 cggtcttcgg actcgccttt cacgtctacg atcccgagcg cctgcttggc ggcgaagccg
     4201 atatcggcat cacctgcgcg ctgattccgg cgacgcatcc gggcgtcgag atcggccgtc
     4261 gccatttccc gcttaacgcc caattcatga acgggccggt gcgcggcaag gacgtcttcg
     4321 taccgctgga cttcatcatc ggcggaccga agatggccgg gcagggctgg cgcatgctca
     4381 tggagtgtct cgcggccggc cgttcgatct cgctgccggc ctcgaacacc ggcatggcgc
     4441 agctgaccgc gcgcgcggtc ggcggttatg cgcgagtccg ccagcagttc ggcatgccga
     4501 ttgggcgctt cgaaggcgtc gaggaggcgt tgacgcggat aggttcgttc acctacctga
     4561 tggatgccac ccggaagatg actgccggtg ccgtcgacct cggcgagaag ccgtcggtgg
     4621 tgtcggcgat cgccaaatac cacgttaccg agcgcgcccg gcaagtggtc aacgacggca
     4681 tggacgttat cggcggcaag ggcatttgcc ttggccccgc gaatatcctc ggccgtgcct
     4741 accagcagat ccccatcggg ctcaccgtcg agggcgccaa catcctcacc cgcagcctga
     4801 tcatcttcgg ccagggggcg atccgctgtc atccgtatgt gctcgccgaa atgaaggcgg
     4861 cgcaggacag caataccgac cgcggccttg agcacttcga ctcggcgctg tggggccacg
     4921 tcgtcttcag cattcgcaac ggcgcgcgcg ccctctggct ggggatgacc ggatctcatt
     4981 tcgcaccggt gcccgacggc tactccgagg aaaccgcgcg ctacgtgcag caactgacgc
     5041 gtttttcaag tgcattcgcc ttcctttccg acgtctcgat gctggtgctc ggcggcggcc
     5101 tcaagcgccg cgaaaagcta tcggcacggc ttggcgacat cctctcgcag atgtacctga
     5161 tgtcggcgac gatcaagcgg ttcgaggccg aaggtcgtca gtcggccgac gcgccgctga
     5221 tgcactgggc aatctgggat tcgatgtacc gcgcgcagga agccttcgac ggtgtcctcg
     5281 ccaactaccc gaaccgggtc gtcgcttggt tcctcaagcg gatcatcttc ccctggggcc
     5341 atccctacgt cgtgccgtcc gatgcgttgg gccacgccgc cgcgcgcctg ctaatcgacg
     5401 caacaccgac ccgtgagcgc ctgaccgccg agacttacgt tgccggcgac gccaacgacc
     5461 cggtcgcggc gatcgaggcg gccttgcgtg cgagcatcga agccgagccg atcgacacga
     5521 agatccgtgc cgccgatcgc gacgggcgct ttgccgacaa tcccggcgcg acggtccgcg
     5581 acatggccga tgtcgcgctt gcagcaggcg tcattgatca ggctgagcac gccaagctca
     5641 agcggcgcaa cgcgctgcga gacaaggtca tccacgtcga tgacttcccc ttcgacctcg
     5701 gcatcagcgt gcgccggcca gaacagggcg acgcgcagcg ggaagcggca tgagcaaagc
     5761 ccccgttttc gtcgtcgatg gtgcgcgcac gcccttcctc aaggcgcgcg gtgcgccggg
     5821 gcagttctcg gcatccgatc tcgccgtgca ggcggggcgg gcactgcttc tgcgtcagcg
     5881 cttcaatgcc gaagacctcg acgaggtgat cctcggctgt gctgcgccgg ccgccgacga
     5941 ggtcaatatc ggccgcgtct gcgccctgcg catgggctgc ggcaacaagg ttccggcctg
     6001 gaccgtcatg cgcaactgcg cctcgggcat gcaggcaatc gattcggcgc tcgctaacat
     6061 ccagtcgggt cgggccaacc tggtgatggc cggcggcgtc gatgcgctgt cacgcgcgcc
     6121 gatcctgttc tccgacgcga tggtcaattg gctcgccagg tggtcgaccg cgcgcggcgt
     6181 cggcgccaag ctcgcagcgc tgcgtggctt ccggccgggc tacctggcac cggttattgg
     6241 cctgctcaaa gggctgaccg atccggtggt aggcctgtcg atgggccaga ccgccgagaa
     6301 cctcgcctgg cgttttggca tcgaccggct ggcgatggat gccttcgccg ccgagagcca
     6361 cctccgcgca ctagccgccc gcaaggccgg cgccttcggc gagatcgtcc cgctgctcga
     6421 ccgtaacggt gtcgtttacg ctgaagacga cggcgtgcgc gaagactcga cgcttgaccg
     6481 cctgggcaag ctacgcccgg tcttcgacaa gccctacggc aacgtgacag ccggcaacag
     6541 ctcgcaggtg accgacggtg ccgcctggct gctgctggcc tccgaggccg cagttacccg
     6601 cttcggcctg acgccgatcg gacgaatact cgacagcgaa tgggccggcc tcgatccggc
     6661 cgaaatggga ctcggcccgg tccatgcggc aacaccgatc ctgcagcgca acggtctggg
     6721 cctggccgac ctcgacctgc ttgaaatcaa cgaggccttc gcggcgcagg tacttggctg
     6781 cctcgccgcc tgggagtcgg cggcctactg ccgcgatggg ctggggctcg atgcgccgct
     6841 gggcaaggtc gatcgcgcgc ggctcaaccc cgacggcggc gccatcgctg tcggtcatcc
     6901 ggtcggtgcc tcgggcgcgc gaatcgttct ccatctcctg cacgcgttgc gtgccagggg
     6961 tggcggtcgt ggtgtcgcgg cgatttgcat cggcggcggt ctcggcgggg cgatgctcgt
     7021 agatacccag acaactcaga cgggaggggc ggcatgagcg aagtcgaata ccgccactgg
     7081 cggatcgaga atgactcgcg cggggtgact tggctcacgc tcgatcgcga aggtgaaacc
     7141 accaacgcgc tgtcggcggc ggtgatggcc gagctcgatt gcatcgtcag cggtttcgag
     7201 cgaaattccc cggcggcgct gatcatccgc tcggcgaagg aagccggatt catcgccggc
     7261 gctgacatcc gcgagttctc ggcgctgaat acaaccgagg gcgcgcacga actgctggcg
     7321 cgcggctggt cgctgttcaa ccgcatcgcc gatttccgtt ttccgactgc ggcgcttatt
     7381 cgcggccatt gtctgggcgg cggcctcgaa ctggcgctcg cctgccgcta ccgcatcgtc
     7441 gtcgacgaac cgtcgacgcg gctcggcctg cccgaagtga tgctcggcat ctttcccggt
     7501 tggggcggca tgcgccgtct gcctaagctg atcgggccgc agctcgcgct agacatgatg
     7561 ctcaccggca agacgctcga cgcccgccgg gcaaagcgca gcggtctcgc cgatctggcg
     7621 gtgccgccgc gcgtcatggt gcgcagcgct gtcgagcacg tgctctcggg caaggcgcca
     7681 cggcgaccgg ccatgctgac gcgtctccta aacgggccgc tgaagcgggt cgtcgccaag
     7741 tcagcgcgca agcaggtggc gacgaaggcg cggcccgaac actacccggc gccctacgcg
     7801 atcatcgact gctgggaaaa ccacgacggc gacgcgttgg cgattccaga ctacctagac
     7861 gccatcgttt cgtcgcccac cgcgcagaac ctcgttcgcg tctttcacct gcaggagcgg
     7921 ctcaagggct tcggcaaggc cgcggatttt ccggttaagc gcgtccacgt catcggcgcc
     7981 ggcgtcatgg gcggcgacat cgccgcctgg tgcgcgctgc gcggcctgac cgtgacgctg
     8041 caggaccagt cggtcgaacg gattgcgccg gcgatcaagc gcgcggcaac actgtactcg
     8101 cgccgcctgc gcgatcgcct agccgaacgc aacgcgctcg accggctgat tcccgacccg
     8161 cgcggcgacg gcgtcggcct cgccgatgtc gtcatcgagg cgatcttcga aaacctcgaa
     8221 gcgaaacacg cgctgttccg cagcatcgag ccgcggctca agcccggcgc cgtgcttgcc
     8281 agcaatacct caagcctgcg cctcgaggac ttgcgtaccg tactcgcgca acccgagcga
     8341 ctcgtgggca ttcacttctt taatccggtg gcgaagatgc cactggttga agtggtcgag
     8401 gccgaaggcg gcgatgaagc cgagcatcga aaggcctgcg cctttgtacg caccatcgac
     8461 aagctaccgc tgccggtaaa gagcgccccg ggcttcctcg ttaacgcggt tcttgcgccc
     8521 tacatgctgg aagcgatgcg cgccgtcgac gagggtgtcg caccggaaaa catcgatgct
     8581 tcgatgctcg cctttggcat gccgatgggg ccgatcgagc tcgccgactc cgttggcctc
     8641 gacatcgccc tcgcggccgg tcgcgaactg gccggcggcg gcgacgcgcc ggcctgcctg
     8701 ttggctcatg tcgaagccgg cgatctcggc aaaaagaccg gcaagggctt ctatacctgg
     8761 gtcgatggca aaccgcagaa gggcgaagcg cgcaccgagc ccgggctcgc cgaccgtttc
     8821 gttcaaccgc tgctcgaccg gacgcaggcg ctggtggccg agcgcgtcgt cgccgatccg
     8881 gacctggccg acgccggcgt gatcttcggc accggcttcg caccgtttac cggcggcccg
     8941 atgaactacc tgaaagcacg caatggctga agaaccggtc atgttgccgc cgggcatacc
     9001 ggcgctacgg gtcatgacca tgcctgcgga cgccaacccc agcggcgaca tcttcggcgg
     9061 ctgggtcatg gcccaggtcg atgtagccgg cgcggtgccg gcaatgcgtc gcgcgcgcgg
     9121 tcgggttgcg acggtcgcgg ttaattcatt tttgtttaaa caacccattt cggtgggtga
     9181 cctcgtcagc ttctatgctg aggttgttag tgagggaaag acgtcgatca cggtcaacgt
     9241 cgaggtttac gccgagcgcc atccggccgc gccgctcatc gtcaaggtga ccgaagcgca
     9301 gctgacctat gtcgcgctcg acggcaaggg gaacaaacgg caactgccgt aaggggattg
     9361 ccggcaaagc cggcgaggta acggagccaa ctccggaaaa cgcgaaagag gagacgaaat
     9421 gaaatcgatg ttgaaagtga agtggatatc aatcgccgtt gccacggcgt tttccgggag
     9481 tgccatggcg gcaggcttcg cgctgcagaa ccagaacggt tcgggcaacg gtaatgcgtt
     9541 cgccggtgca gcagcagcgg ccgaggacgc cgggacgatt ttctttaacc cggcgggtat
     9601 ggcgctgctg ccgcagggca aaaacgttgc ggtagcgggg acctatctgg atcgttcggt
     9661 gaaattcacc gatgctggga ccagcccgcc gaccctgagc accacaacaa gcacaaacgg
     9721 cggagatgcg ggtggatcg
//
LOCUS       DATDAA010000060         9795 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_144053,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000060 DATDAA010000000
VERSION     DATDAA010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9795
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144053"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="VIS73_02000"
     CDS             complement(<1..1130)
                     /locus_tag="VIS73_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY5761959.1"
                     /translation="MSQLIEVKVPDIGDFSDVPVIDVFVKPGDTVAVDDALVTLESDK
                     ATMDVPSSAAGVVKEVLVKLGDKVSEGSLVIRLEAAGAAAAAPAPAKAAPAAPAPTAA
                     APAAGGGVVEVKVPDIGDFTDVPIIDLFVKVGDTIAVDDAIVTLESDKATMDVPATAG
                     GVVKEVLVKLGDKVSEGTALVRVESAGTGTATPAAAPAKAAPPAAAAPAPAAAAPAPS
                     APAGAPAPTLPLGAKLHASPSVRAYARELGVDLTKVSASGPKNRIVKEDVTAFVKGVM
                     SGGGAAAATGGSGAITGGGTLNLLPWPKVDFSKFGEIEVKELSRIKKLSAANLARNWV
                     MIPAVTYHEDADITDLEAFRVQVNKENEKSGKKLTMLAFLMKA"
     gene            complement(1145..3853)
                     /gene="aceE"
                     /locus_tag="VIS73_02005"
     CDS             complement(1145..3853)
                     /gene="aceE"
                     /locus_tag="VIS73_02005"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY5761960.1"
                     /translation="MAQSPGAAASAVPAANPADADPQETREWVDALQGVITQEGPQRA
                     HFLIETLIGQAREDGIDIPYSATTEYINTIPVELQARIPGDQNIEHTIRSYTRWNAMA
                     MVLRANKDTNVGGHIASFASSATLYDVGFNHFWHAPGSEHGGDLVYFQGHSVTGVYAR
                     AFMLGRLSEEQLDNFRQEVDGKGVSSYPHPWLMKDFWQFPTVSMGLGPLQAIYQARFM
                     KYLASRGFIDATKAEKRKVWAFLGDGETDEVESLGAIGMAAREKLDNLIFVINCNLQR
                     LDGPVRGNGKIIQELESEFRGAGWNVIKVIWGTHWDALFARDKKGILRKRMMEIVDGE
                     YQTFKAKDGAYVREHFFNTPELKALVADWSDDDIWQLNRGGHDPHKIYAAFDMAVKHK
                     GQPTLILAKTIKGYGMSSSGEAQNTSHQQKKMDKDSIRRFRDKFGLPVKDEDLEKLPF
                     LTFPKGSPELNYMAERRHALGGYLPNRPHAAPALKVPELAAFDALLKASGDGRELSTT
                     MAIVRIMNILLKDKAVGKHIVPIVPDESRTFGMEGMFRQVGIWNQEGQNYVPQDHDQL
                     MFYKESKTGQILQEGINEAGAMADFIAAGTAYSVHGVQMIPFYIFYSMFGLQRTMDLC
                     WAAADQRTRGFLIGGTAGRTTLNGEGLQHEDGHSQILAGSIPNCIAYDPTFQFEVAVI
                     AQDGLRRMFVEQEDIYFYITVMNENYEHPEMPKGAEADILKGMYALKKGKAGKKGAPR
                     VQLLGSGTIFREVIAAADLLAKDWGVESDLWGCPSFNELARDGQDVERWNLLHPTDKP
                     KQSHVAKCLDKTDGPVIAATDYVRLFAEQIRPFVNRRYVTLGTDGFGRSDTREKLRHF
                     FEVDRHWVTVAALKALADDGAIEHKKVAEAIKKYGLDPKKPNPMTV"
     gene            4106..4492
                     /locus_tag="VIS73_02010"
     CDS             4106..4492
                     /locus_tag="VIS73_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY5761961.1"
                     /translation="MTLLWRKQLEIGDDRIDTDHKYLICLVNTVELSLRTQQHRDILM
                     ATLDQLAEYTELHFEREESIMLSIRYKRYDQHRQQHQLLIKELEAIGKRIEAHAEDAL
                     SDEECEVLDTVSPLDRRPYRQRRHAA"
     gene            complement(4409..4954)
                     /locus_tag="VIS73_02015"
     CDS             complement(4409..4954)
                     /locus_tag="VIS73_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761962.1"
                     /translation="MASAESLQAFGKHPRAVGPTAEQERGELSAAEATSAIGLAQALA
                     NGFGECAQDEVDGSLAVGIVDSLEVVKVEHPHDSVGTGPLEAGEVLFEPLHQVSSIGQ
                     PVQQISLCHPRQFFLKRVALGDVALHAEVTDDATPIVGKQLEMALRYACSASLSCMSS
                     LTIWSTIQRRNSVENLALLVA"
     gene            complement(5051..6955)
                     /locus_tag="VIS73_02020"
     CDS             complement(5051..6955)
                     /locus_tag="VIS73_02020"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEY5761963.1"
                     /translation="MIAAASRLTAILALLCWSIAGFANERIVDFHAEVDVDRSGSLSV
                     RETIVVIAAGDQIKRGIYRDFPTRYLRPWGRVVVPFSVLSVQRNGVAEPFRVEQKDEG
                     VRVYIGRADRQIPPGEHEYVLTYRTARQLGFFDTHDELYWNVTGNDWAFPIESASATI
                     RVPMALSAANATIEAYTGFKGDQGSDFNAHFDESGVAQFATTQALLSGQGLTVVVGWP
                     KGIVREPDASEQRANWLADNPELRVGAWGVVVLFAYYLAIWLWRGRDPAPGTIIPRYK
                     PPAGVSAADARYLSQMGFDQRVVAAGVIGLAIKGWLQVHESNGSFTLVAGTGKEPPTN
                     DEQALLDALALGKDGSLGISNAHASRFKEASARLKAELKREHLKRHFITNAPWTIVGL
                     AASAALLIGAAFAGGAMAPESAFLILWLTGWTFGVYALLGGVLRSWGDVLRGRLAMAV
                     PAVFLSLFSLPFVGAEIFVFGLLFASAPALLLLLFLLVGINYAFYRWLKAPTRLGRPL
                     YDQLAGFRMYLDLAEKDRLNLLNPPEETPALFERYLPYALALGVEQRWSERFASVMAA
                     AGMAGEHAPHWYHGDAWNPHALGNFSRSLGSGFAGALANAASPPGSSSGGGGGGSSGG
                     GGGGGGGGGW"
     gene            complement(6952..7515)
                     /locus_tag="VIS73_02025"
     CDS             complement(6952..7515)
                     /locus_tag="VIS73_02025"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY5761964.1"
                     /translation="MPDLPPSLLVATGLLLALAIGAILIFNRLVARRHHASAAWSDID
                     VQLKRRHDLLPKLAAAVSRYAEFEASVQQSVAALRDLPQAPAGRADLENRVTGALGSL
                     FAVAEAYPELKTSEQFLALQSQISEVEEQIQYARRYFNGAVRELNILVQSFPSNLVAR
                     LTGFASLDYFRIELATQRESPELELDR"
     gene            complement(7651..8256)
                     /locus_tag="VIS73_02030"
     CDS             complement(7651..8256)
                     /locus_tag="VIS73_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY5761965.1"
                     /translation="MSPTPPPVDPTAYFPPPDPRHWAARLLRAFGWTLELAPVPATHV
                     MVAVYPHTSNWDFPLGLLTRFACRWPIHWVGKAAIFAGPWRGLLRRWGGIPVDRSAAE
                     GFAEALAGEFARRDHLMVAIAPEGTRRHVDALKSGFYRIALAANAPIGLGYIDYGKRR
                     IGIGGYFTPSGDTAADLEIMQRFYADKRGRYPAQAGNIRFR"
     gene            complement(8273..9067)
                     /locus_tag="VIS73_02035"
     CDS             complement(8273..9067)
                     /locus_tag="VIS73_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761966.1"
                     /translation="MSTSAAESARAVHSIVFTLADPAALGVDQESASAALQRLDHLVR
                     QALTNSRAGAEHLIGNEFGILVSFLNSGAALTFVRVLQHLAGGDGGLPIRIGVHMGSL
                     GADQQQIEQRPQLRLTRQIAALARTGQALASPLFHNFVTQFDHRCKSMLQAMPPVVDT
                     QGRNMVVYEINFAGLPALRLPNANNAGAEPVADPVALPADDGRRQEFLQLVERALAEE
                     IGPLAQFIVRQAADTAHTRAAFYRIVGETLPETVQRAEFFKTLDRL"
     gene            complement(9064..>9795)
                     /locus_tag="VIS73_02040"
     CDS             complement(9064..>9795)
                     /locus_tag="VIS73_02040"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEY5761967.1"
                     /translation="LAIRGYTVSQIKPVLDPFLAERFLPQTVPAFSATETLLALKAKY
                     PEFDYKEATLDPTNPRDKADLIEAEIVYRFRNDRALTEIRGERFVGNRRTLYVARPIE
                     IKSTACLQCHNTPENAPATMLAVYGSQGGFGWKVGDIVGAQIITVPTEVADRNAMRAF
                     ATFMAAQAAVFLMIFIVLNVMLNRFIIVPIRRISTVADEISTGRTEAPEFAADGHDEV
                     AGLEKSFNRMRRSLTLAIKMLEDKP"
BASE COUNT         1621 a         3037 c         3372 g         1765 t
ORIGIN      
        1 gccttcatca ggaaggcgag catggtgagc ttcttgccgc tcttttcgtt ctccttgttc
       61 acctgcacgc ggaatgcctc gagatcagtg atgtcggcgt cctcgtggta ggtgacggcc
      121 gggatcatca cccagttgcg cgcgaggttg gcggccgaga gcttcttgat ccgcgatagc
      181 tccttgacct cgatttcgcc gaacttcgag aagtcgacct tgggccaggg cagcaggttg
      241 agcgtgccgc cacccgtgat cgcgccagag ccgccggttg cagcggcggc tccgccgccg
      301 ctcatcacgc ccttgacgaa ggcggtgacg tcttccttga cgatgcggtt ctttgggccg
      361 ctcgccgaaa ccttggtcag gtcgaccccg agttcgcgcg cgtaggcgcg caccgagggc
      421 gaggcgtgca atttggcacc gagcggcagt gtcggcgccg gtgcgccggc gggtgctgat
      481 ggggcgggcg cagcggcggc gggagccggg gcggcagcgg ccggtggtgc cgccttggcc
      541 ggagcggctg ccggggtcgc tgtgcctgtg ccggcggatt cgacgcgaac cagcgccgtg
      601 ccttcggaga ccttgtcgcc gagcttgacc agcacctcct tgaccacgcc gccggcggtg
      661 gccggtacgt ccatcgttgc cttgtccgac tcaagtgtga cgatcgcgtc gtccaccgca
      721 atggtgtcgc cgaccttgac gaagaggtcg atgatcggca cgtcggtgaa gtcaccgatg
      781 tcgggtacct tgacctcgac gacaccgccg ccggctgccg gtgcggctgc ggtgggagcc
      841 ggcgctgccg gtgcggcctt ggctggggca ggggcggcag ccgcggcgcc ggctgcttca
      901 aggcgaatca ccagcgatcc ttcgctgacc ttgtcgccga gcttgaccag cacttccttg
      961 accacgcccg ccgccgacga gggaacgtcc atcgtcgcct tgtccgattc gagcgtgacc
     1021 agcgcgtcgt ccaccgcaac tgtgtcgccg ggcttgacga agacgtcgat gaccggaacg
     1081 tcggagaaat cgccgatgtc ggggactttt acttcgatca gttggctcat gttcttctct
     1141 cccctcagac cgtcatcgga ttcggcttct tcggatcgag gccatacttc ttgatggcct
     1201 cggcgacctt cttgtgttcg atcgcgccgt cgtcggcgag cgccttcaag gcagcgaccg
     1261 tcacccagtg ccgatcgacc tcgaagaagt ggcgcagctt ctcgcgcgtg tccgagcggc
     1321 cgaaaccgtc ggtgcccagc gtcacgtagc ggcggttgac gaagggacgg atttgctcgg
     1381 cgaagaggcg gacgtagtcg gtcgccgcga tcaccggccc gtcggtcttg tcgaggcact
     1441 tggcgacgtg cgactgcttg ggcttgtcgg tcgggtggag caggttccag cgttcgacgt
     1501 cctgtccatc gcgcgccagc tcgttaaacg atgggcagcc ccagaggtcg gactcgacgc
     1561 cccagtcctt ggccagcagg tcggcagcgg cgatgacttc gcggaagatc gttccggagc
     1621 cgagcagctg aacgcgtggt gcgcccttct tgccggcctt gcctttcttc agcgcgtaca
     1681 tgcccttgag gatgtcggcc tcggcaccct tcggcatctc cggatgttcg taattctcgt
     1741 tcatcacggt gatgtagaag tagatgtctt cctgctcgac gaacatgcgg cgaaggccgt
     1801 cctgagcgat taccgcgacc tcgaactgga aggtcgggtc ataggcgatg cagttgggga
     1861 ttgagccggc gaggatttgc gagtgcccgt cttcgtgctg caggccctcg ccgttcagcg
     1921 tcgtgcggcc ggccgtgccg ccgatcagga aaccgcgcgt gcgctggtcg gccgctgccc
     1981 agcagaggtc catcgttcgc tgcaggccga acatcgaata gaagatgtag aaaggaatca
     2041 tctgcacgcc gtgcaccgag taggcggtgc cggcggcgat gaagtcggcc atcgcgccgg
     2101 cttcgttgat accttcctgg aggatctggc cggtcttcga ttccttgtag aacatcagct
     2161 ggtcatggtc ctgcggaacg tagttctggc cttcctgatt ccagatgccg acctgccgga
     2221 acatcccctc catgccgaag gtgcgtgatt cgtcggggac gatcgggacg atgtgtttgc
     2281 ccaccgcctt gtccttcagc aggatgttca tgatgcgaac gatcgccatc gtcgtcgaca
     2341 gttcgcgccc gtcgcccgag gccttgagca gcgcgtcgaa ggcggccagt tcgggcacct
     2401 tcagcgccgg cgccgcgtgc gggcggttgg gcagatagcc accgagtgca tgacggcgct
     2461 cggccatgta gttgagttcg ggtgagccct tggggaatgt gaggaagggc agcttctcta
     2521 ggtcctcgtc cttgaccggc aggccaaact tgtcgcggaa gcggcggatc gagtccttgt
     2581 ccatcttctt ctgctggtgc gaggtgttct gcgcctcgcc cgagctgctc atgccgtagc
     2641 ccttgatcgt cttggcgagg atcagcgttg gctggccctt gtgcttcacc gccatgtcga
     2701 aggctgcgta gatcttgtgc gggtcgtggc cgccgcggtt gagctgccag atgtcgtcgt
     2761 cagaccagtc ggcgaccagc gccttcagtt caggcgtatt gaagaaatgc tcgcggacgt
     2821 aggccccgtc cttggccttg aaggtctgat actcaccatc aacgatttcc atcatgcgct
     2881 tgcgcaggat gcccttcttg tcgcgggcga acagcgcgtc ccagtgcgta ccccagatga
     2941 ccttgatgac gttccagccg gccccacgga actccgattc cagctcctgg atgatcttgc
     3001 cgttgccgcg aaccgggccg tcgaggcgct gcaggttgca gttgatgacg aagatcaggt
     3061 tgtcgagctt ctcgcgtgct gccatgccga tcgcgccgag gctctcgacc tcgtcggtct
     3121 cgccgtcccc gaggaaggcc cagaccttgc gcttttcggc cttggtcgcg tcgatgaagc
     3181 cgcgcgaggc gaggtacttc atgaaacggg cctggtagat cgcctgcaag gggccgaggc
     3241 ccatcgacac tgtcggaaac tgccagaaat ccttcatcaa ccaggggtgc gggtaggagg
     3301 agacgccctt gccgtcgacc tcctggcgga agttatcgag ctgctcttcg ctgagccgcc
     3361 cgagcatgaa ggcgcgcgcg taaacaccgg tgaccgagtg gccctggaaa tagacgaggt
     3421 cgccgccgtg ttccgaaccg ggcgcatgcc agaagtggtt gaagccgacg tcgtagagcg
     3481 ttgccgagga ggcgaagctg gcgatgtggc cgccgacgtt ggtgtccttg ttggcgcgca
     3541 gcaccatcgc catggcgttc cagcgcgtgt agctgcgaat cgtgtgctcg atgttctggt
     3601 cgccgggaat gcgtgcctgc agttcgaccg gaatcgtatt gatgtactcg gtggtggcgc
     3661 tgtagggaat gtcgatcccg tcttcgcgcg cctggccgat tagcgtctca atcaggaagt
     3721 gggcgcgttg cggtccttcc tgcgtaatga cgccctgcag ggcgtcgacc cattcacggg
     3781 tttcctgggg gtcggcatcg gcggggttgg cggcgggaac ggcggaagcg gcggcgccgg
     3841 gtgactgggc catggtgtgt ctcctcgttg tttttgtcgg aagcagccta aaggataacc
     3901 ctcaagggat ttcggacaag gggaaggggc tgcgggtttc aatattttgt gacaggcaat
     3961 gacattccgg gatgcgacga gttcctgacg gttccatttc ctgccactgg cgcaattgac
     4021 ccacgcgtca agtcgatgga ccctggcgcc gcggcgcaca tggggcgtgc cgccaacgac
     4081 aatcagtccg gaccgggaga aggatatgac gctgctgtgg cgcaagcaac tcgaaattgg
     4141 cgacgatcgg atcgatacgg atcacaagta cctcatctgc ctcgtcaaca ccgtcgagct
     4201 gtcgctgcgc acccagcagc accgcgacat cctgatggcc acgctcgacc agctcgccga
     4261 gtacaccgag ctgcacttcg aacgcgagga gtcgatcatg ttgtcgatcc gctacaagcg
     4321 ctacgaccag catcgccagc agcatcaact gctgattaag gagctcgaag cgattggcaa
     4381 gcggatcgag gcccacgccg aagacgcgct aagcgacgag gagtgcgagg ttctcgacac
     4441 tgtttcgccg ctggatcgtc gaccatatcg tcaaagaaga catgcagctt aagctcgcgc
     4501 tgcacgcgta gcgcagcgcc atttcaagct gcttaccgac gattggggtc gcatcgtcgg
     4561 tcacttcggc gtgtagcgcg acgtcaccga gcgccacgcg cttcaggaag aactgacgcg
     4621 gatggcacaa actgatctgc tgaacgggct gcccaatcga cgacacctga tggagcggct
     4681 cgaacaggac ttcgcccgct tccagcgggc cggtgccgac gctgtcgtgc ggatgctcga
     4741 ccttgaccac ttcaagcgag tcaacgatgc ccacggccag gctgccgtcg acctcgtctt
     4801 gcgcgcattc gccgaagccg ttcgccagag cctgcgcaag accgatagcg ctggtcgcct
     4861 cggcggcgct gagttcgccg cgctcctgct cggcagtggg accgacggcg cgcgggtgtt
     4921 tgccgaacgc ctgcagcgac tcggctgaag ccacgctggc acgcgctgat gccgccctct
     4981 accgcgccaa gtccggtggc cggaaccggg tcgaggtcga tgcagggccc gatctcgacg
     5041 gttgggccaa ttaccagccg ccgcctccgc cgccgccacc accgccccca gaactacccc
     5101 cgccgccccc gccgctactg ctgccgggcg gacttgcggc attggccagg gcgccggcga
     5161 agccgctgcc cagcgatctg gaaaaattgc ccagcgcatg cggattccag gcgtcaccgt
     5221 ggtaccagtg gggcgcgtgt tcgccggcca ttcccgcggc agccatcacg ctggcgaatc
     5281 gttcgctcca gcgttgctcg acgccgagcg ccagcgcgta gggcaggtag cgctcgaaca
     5341 gcgccggtgt ttcctcgggt ggattgagca ggttgagtcg atccttctcg gcgaggtcga
     5401 ggtacatgcg gaatccggcg agctggtcgt agagcggccg gcccagtcgc gtcggcgcct
     5461 tgagccagcg atagaaggca tagttgatgc cgaccagcag aaagagcaac aggagcaagg
     5521 caggtgcgct ggcgaagagc aggccgaaga cgaagatctc ggcgccgacg aagggcaggc
     5581 tgaacaacga gaggaagact gccggcactg ccatcgccag ccgcccgcgc aggacatcgc
     5641 cccagctgcg cagcacgccg ccgagtagcg cgtagacacc gaaggtccag ccggtcagcc
     5701 acagaatcag gaaggcgctt tccggcgcca tcgcaccgcc ggcaaacgcc gcgcctatca
     5761 gcagcgccgc gctcgcggcc agaccgacga tggtccacgg tgcgttggtg atgaagtgcc
     5821 gcttcaggtg ttcgcgcttg agttcggcct tgagccgggc gctcgcctcc ttgaagcgcg
     5881 acgcgtgggc attgctgatg ccaagcgaac cgtccttgcc gagtgccagc gcgtcgagca
     5941 gcgcttgctc gtcgttggtc ggcggctcct tgcccgtacc ggcgaccagc gtgaagctgc
     6001 cgttggactc gtggacctgc agccagccct tgatcgccag cccgatcacg ccggcggcga
     6061 cgacgcgctg gtcgaagccc atctgcgaca gatagcgggc atcggcggcc gagacgccgg
     6121 ccggcggttt atagcgcggg atgatcgtcc ctggcgccgg atcgcggccg cgccagagcc
     6181 agatcgccag atagtaggca aatagcacga ctacgcccca ggcgccgacc cgcagctcgg
     6241 gattgtcggc gagccagttg gcacgctgct cgctcgcgtc aggttcgcgg acgatgccct
     6301 tgggccagcc gacgacgacg gtcagcccct ggccgctgag caacgcttgc gtcgtggcga
     6361 actgcgcgac tcccgattcg tcgaaatgtg cgttgaagtc gctgccctgg tcgcccttga
     6421 agcccgtgta agcctcgatc gttgcgttcg ccgccgacag cgccatcggc acgcggatcg
     6481 tcgctgaggc tgactcgatg gggaatgccc agtcgttgcc ggtgacgttc cagtacagct
     6541 cgtcgtgcgt gtcgaaaaag ccgagctgtc gtgccgtccg gtaggtcagc acgtactcgt
     6601 gctcgcccgg cggaatttgc cggtcggcgc gcccgatgta gacgcgcacg ccttcgtcct
     6661 tctgctcgac gcggaagggc tcggcgacgc cgttgcgctg caccgagagt accgagaagg
     6721 gcacgacgac acggccccaa gggcgcaggt agcgcgtcgg gaaatcgcgg tagatgccgc
     6781 gcttaatctg atcgccggcg gcgatgacga cgatcgtttc gcgaacgctg agggatccgg
     6841 aacggtcgac gtcgacctcg gcatgaaagt cgacgatgcg ctcgttggcg aaaccggcga
     6901 tgctccagca gagcagcgcg aggatcgccg tcagacgcga ggccgcggca atcaccggtc
     6961 gagctccagt tccgggctct cgcgctgggt cgccagttcg atccggaagt agtcgagcga
     7021 ggcgaaaccg gtgagccggg cgacgaggtt ggacggaaag gactggacga ggatgttcag
     7081 ctcgcgcacc gcgccgttga aatagcgccg cgcgtactgg atctgctcct cgacttcgga
     7141 gatctgcgac tgcagcgcga ggaactgctc gctggtcttc agctccgggt aggcctcggc
     7201 gacggcgaag agcgagccga gtgcgccggt tacgcgattc tcgagatcag cgcgcccggc
     7261 cggtgcctgc ggcaggtcgc gcagcgctgc caccgactgc tgcacgctgg cctcgaactc
     7321 ggcgtagcgg ctgactgcgg cggcaagctt gggcagcaga tcatggcgcc gcttgagttg
     7381 cacatcaatg tcgctccacg cggcgctggc gtggtgccgg cgcgcgacca gtcggttgaa
     7441 gatcaatatg gcgccaatcg ccagcgcgag cagcaggccg gtggcgacaa gcagggatgg
     7501 cggcaggtcg ggcatggtgg gcgcaggagg cggaggaccg ccggactcta ctgcgtttgc
     7561 gcgggccttc caagccgccg ccgattggtg agcgcaggcg gcaaggcagg ttgcggcaga
     7621 ggcgtccgtc cgcccggctg tcgcgtggaa tcagcgaaac cggatgttcc cggcctgcgc
     7681 cgggtagcgg cctctcttgt cggcgtagaa gcgctgcatt atttcgaggt cggctgccgt
     7741 atcgccgctc ggcgtgaagt agcctccgat gccgatgcgg cgctttccgt agtcgatgta
     7801 gccgagcccg atcggcgcgt tggcggcgag cgcgatgcgg tagaagccgg acttgagtgc
     7861 atcgacgtga cggcgcgtgc cttcgggggc gatcgccacc atcaggtgat cgcggcgcgc
     7921 gaattcgccg gcgagcgcct cggcaaagcc ttcggcggcg ctgcggtcaa ctggaatgcc
     7981 gccccagcgg cgcaacaggc cacgccatgg cccggcgaag atcgccgcct tgccgaccca
     8041 gtggatcggc cagcgacagg caaagcgggt gaggaggccg agcgggaaat cccagttgga
     8101 ggtgtgagga tagaccgcca ccatgacatg ggtggcgggc accggcgcca gttcgagcgt
     8161 ccagccgaag gcgcgcagca gacgtgccgc ccagtgtcga gggtcgggcg gcgggaagta
     8221 ggcggtaggg tcgaccggcg gcggcgtggg gctcatcgcg tcgctcgatg gctcaaagcc
     8281 gatcgagcgt cttgaaaaac tcggcgcgct ggacggtttc gggaagcgtc tcgccgacga
     8341 tgcggtagaa ggcggcgcgg gtatgggcgg tgtcggctgc ctggcgaacg atgaattggg
     8401 cgagcggccc gatctcctcg gccagcgcgc gctcgaccag ttgcaggaat tcttggcgtc
     8461 ggccgtcgtc ggcgggcagg gcgaccggat cggcgacggg ctcggcgccg gcattatttg
     8521 cgttcggcag gcgcagtgcc ggcagcccgg caaaattgat ctcgtagacg accatgttgc
     8581 gtccctgcgt atcgacgacc ggcggcatcg cctgcagcat cgacttgcag cgatggtcga
     8641 actgggtgac gaagttgtgg aagagcggag aggccagcgc ctgcccggtt cgcgccagcg
     8701 cggcaatctg tcgggtcagc cgcagttgcg ggcgttgctc gatctgctgc tggtcggcgc
     8761 cgagcgaacc catgtgcacg ccgatgcgga tcggcaatcc gccatcgccg ccggccaggt
     8821 gctgaaggac gcggacgaag gtgagcgccg cgcccgagtt gaggaaggaa acgagaatgc
     8881 cgaactcgtt gccgatgagg tgctcggcac cggcgcggct gttggtcagc gcctgccgga
     8941 cgaggtggtc gaggcgttgg agcgcggcac tggcgctctc ctgatcgacg ccgagcgcgg
     9001 ccggatcggc cagcgtgaag acgatgctgt ggaccgcgcg tgccgattcg gcggcggaag
     9061 tgctcatggc ttgtcttcca gcatcttgat cgccagcgtc aggctgcgtc gcatgcggtt
     9121 aaaggatttc tccagccccg ccacttcgtc gtggccgtcg gcggcaaatt caggcgcctc
     9181 ggtccgcccg gtgctgatct cgtcggcgac cgtcgagatt cgccggatcg gcacgatgat
     9241 gaagcggttg agcatgacgt tgaggacgat gaagatcatc aggaagaccg cggcctgcgc
     9301 ggccataaag gtggcaaagg cacgcatcgc atttcggtcg gcaacctcgg tcgggacggt
     9361 gatgatctgc gcgccgacga tgtcaccgac tttccagccg aaaccgccct gtgaaccgta
     9421 gacagcgagc atcgtcgccg gcgcgttctc gggcgtgttg tggcactgca ggcaggccgt
     9481 gctcttgatt tcgatggggc gcgcgacgta gagcgtgcgc cggttgccga cgaagcgctc
     9541 gccgcggatt tcggtgagcg cgcggtcgtt gcggaaacgg tagacgatct cggcttcgat
     9601 gaggtccgcc ttgtcgcgcg gattggtcgg atcaagcgtc gcttccttgt agtcgaactc
     9661 ggggtacttg gccttaagcg ccagcagcgt ttcggttgcc gagaacgccg ggacggtctg
     9721 cggcaggaag cgctcggcga ggaagggatc gagcacgggc ttgatctggc ttaccgtgta
     9781 gccgcggatc gccag
//
LOCUS       DATDAA010000061         6736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_179039,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000061 DATDAA010000000
VERSION     DATDAA010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179039"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="VIS73_02045"
     CDS             complement(<1..1349)
                     /locus_tag="VIS73_02045"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEY5761968.1"
                     /translation="MTDLPASLLEETLDEIGTAVQSALRGGRDAIYARLSESASAPGN
                     ADIRNVFDALRGKLVAEWPNGCALFVDGFRERVSDSSGTVGLSSAVRELQLMEDDVVE
                     WDIAINASAQRVATAASDDLSDLEQRLAMIAGDAYDDRAGDYGIVAAATAAGLRKMIE
                     SIAQTLTERRTLLSPLLALMGDPLKTAVSEANKALAERGLNPAAARREAARSKASKKR
                     EANVLGALERLAGPMAGGGGGGGGAGGGGGLPGGFVALPANLVASLNRLQELDQRLLG
                     GAADIDEPVGGSALRAFRQAEGQHLPPMEATMIDLVATIFDLIFQDGQVPDAIKALIG
                     RLQIPLLKIAMSDRSFFSSREHPARAFLDAISQGSVSLGKEIDRDHPYCAQVKLLINR
                     VLDEFEGNPDIFAVLMPELRSLIERTEAEADRLAEKTRQVAEQQEESDLAEIKADEV"
     gene            complement(1534..3339)
                     /locus_tag="VIS73_02050"
     CDS             complement(1534..3339)
                     /locus_tag="VIS73_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY5761969.1"
                     /translation="MAEYVAPLREMRFLMNEVAGLDAVAALPGNEEVSPDLIDAILDE
                     AARFAEEVISPLNVVGDRVGARWEDRKVTMPPGFGDAYRLFAESGWTALSPDSKYGGQ
                     GLPKLVSAAVNEMWKSSNIAFSLCPMLTQGAIEAVMIAGTDALKDKFLPKMVDGTWSG
                     TMNLTEPQAGSDLAAVRTRAEPQADGSYRIYGQKIFITYGEHDMTENIVHLVLGRTPT
                     APEGVKGISLFVVPKFLVGDDGAIGARNDVHCVSIEHKLGIHASPTAVLAFGDGGELG
                     GGAEGYLVGEENRGLEYMFIMMNAARFAVGMEGLAIAERAYQRARDYAKERVQGPEVG
                     VRGGPKVAIIRHPDVRRMLMTMRAQVEAMRALAYVVAAAQDHARHGADEATRARHQAF
                     ADLMIPVVKGWSTETAIEIASTGIQVHGGMGYIEETGAAQYLRDARITAIYEGTTAIQ
                     ANDLIGRKMAREQGATFKAVLADMQKTQKELAALGGDDFGAIAARLATAIAALEAAGN
                     WLLANYAKDIRAAHAGSVPFLRLLGIVAGGWQLFRAAAAAEAHRVGGDADPFWRAKVV
                     TARAYGDHVLTQAVGLAEAIVGGAAGVMGLDDELF"
     gene            complement(3404..4045)
                     /locus_tag="VIS73_02055"
     CDS             complement(3404..4045)
                     /locus_tag="VIS73_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEY5761970.1"
                     /translation="MFAAGIAIALLALALFAAPWARFRDSGLRNAFGAACAALLVLWQ
                     MQFELDSGLRLHLAGATVLTLIVGPWLATVGLGLVAAASSLTLGVSPTPVVDWALRFL
                     GAGGFPVLLTWGVVRILRRFAPAHPFVYIFGNGFFAAGLALAGGGALAGLLGLAGGRG
                     EAATLLEVWLPALSLLAFAEAWLSGMIVTLLVVYRPQWLSSFDASCYLARRGD"
     gene            complement(4049..4981)
                     /locus_tag="VIS73_02060"
     CDS             complement(4049..4981)
                     /locus_tag="VIS73_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY5761971.1"
                     /translation="MTILLIAEHDNKSIKPATRNALTAAASLGGEVSVLVAGADCAAV
                     ASAAASLAGVAKVVHADAPHYADQLAENLAPLVAKLAVSCSHVVAPATTFGKNLMPRV
                     AALLDVAQLSDIVAVQAPDTFVRPIYAGNVLATVQSSDAVKVVTVRTTAFDAAGDGGS
                     AAVETIEAGPDLGLSRVVGRELTVSERPELTAARVIVSGGRGLGSGDKFHSLLEPLAD
                     KLNAALGASRAAVDAGYVPNDYQVGQTGKIVAPDLYIAVGISGAIQHLAGMKDSKVIV
                     AINKDGEAPIFQVADYGLVGDLFEVVPQLLAELG"
     gene            complement(4981..5730)
                     /locus_tag="VIS73_02065"
     CDS             complement(4981..5730)
                     /locus_tag="VIS73_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY5761972.1"
                     /translation="MKVLVPVKRVVDYNVKVRPKSDGSGVDIANVKMSMNPFDEIAVE
                     EAVRLKEAGVATEIVAVSAGLANCQETLRTAMALGADRAILIESSADLQPLAVAKLLK
                     AVVDKEAPKLVICGKQAIDDDANQTGQMLAALLGWPQATFASKLVVASEAATVTREID
                     GGLETLEIKLPAVVTTDLRLNEPRYATLPNIMKAKKKPLDTLTPEALGVDVAPRITQL
                     SVAEPPKRKAGIRVADARELVEKLRNEAKVL"
     gene            5945..6514
                     /locus_tag="VIS73_02070"
     CDS             5945..6514
                     /locus_tag="VIS73_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5761973.1"
                     /translation="MAVRKEVSSLDPQRWIDAAIEILVGGNVDAIRVEVLAKTLGVTK
                     GSFYWHFRDRSDLLARLLAFWRDGRIADITRHAASPPGREREQALRLIELYSANRSRK
                     GIAIELAIRDWARKDEMAAKAVEEVDVVRLDLAGSLLAASGFPPAEARARSLLLYAYV
                     FGQSLMFYDHFDDDLPALKRWVADHIVNR"
     gene            6553..>6736
                     /gene="fliW"
                     /locus_tag="VIS73_02075"
     CDS             6553..>6736
                     /gene="fliW"
                     /locus_tag="VIS73_02075"
                     /inference="COORDINATES: protein motif:HMM:NF014660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HEY5761974.1"
                     /translation="MKCETFLFGSIEIAPESIIEFPNGLPGFPDNQRFALIHEENAGD
                     SPASFTLQSLDDRNIAL"
BASE COUNT         1151 a         2304 c         2132 g         1149 t
ORIGIN      
        1 aagacctcgt cggccttgat ctctgcaagg tcgctctctt cctgttgctc agcgacctgc
       61 cgcgttttct cggcaagccg gtcggcctcg gcctcggtcc gctcgatcag gctgcgcaac
      121 tcgggcatca gcaccgcgaa gatgtccggg ttgccttcga attcgtcgag cacgcgattg
      181 atcagcagct tgacctgcgc gcaatacgga tggtcgcgat cgatctcctt gccgagcgat
      241 accgagccct gagagatcgc gtcgaggaag gctcgcgccg gatgctcgcg gctggaaaag
      301 aagctgcggt cgctcatcgc gatcttgagc agcggaatct gcaggcggcc gatcagcgcc
      361 ttgatcgcgt cgggaacctg gccgtcctgg aagatcaggt cgaagatggt cgcgacgaga
      421 tcgatcatcg tcgcttccat cggcggcaag tgctgtccct cggcctggcg gaaagcgcgc
      481 agcgcactgc cgccgaccgg ctcatcgatg tcggcggcac cgccgagcag gcgttggtcg
      541 agttcctgca gtcgattgag cgaggcaacg aggttcgccg gcaaggccac gaagcccccg
      601 ggcaagccac cgccgccacc cgcgccaccg ccgccccccc ctccacccgc catcgggccg
      661 gccaggcgct ctagggcacc gaggacgttc gcctcgcgct tcttgctggc cttcgaacgc
      721 gccgcctcgc ggcgtgctgc agcgggattc agaccgcgtt cggccagcgc cttgttggct
      781 tccgaaacgg cggtcttcag cggatcgccc atgagcgcca gcaagggtga gagcaaggtt
      841 cgccgttcgg tcagcgtctg cgcgatcgat tcgatcatct tccgcaggcc ggctgctgtt
      901 gccgccgcga cgatgccgta atcgccggct cggtcatcgt aggcatcgcc ggcaatcatc
      961 gccaaccgct gctcgagatc cgacaggtcg tcgctggctg ccgtcgcgac acgctgtgcc
     1021 gaggcgttga ttgcgatgtc ccattcgacg acatcgtctt ccatcagctg cagttcgcgg
     1081 acggcagaac tcaggccgac cgtgccgctg ctgtcactga cacgctcgcg aaatccatcg
     1141 acaaaaagcg cgcaaccgtt cggccactcg gcaaccagct tgccgcgcag ggcgtcgaag
     1201 acgttacgga tgtcggcgtt tcccggggcg ctagccgatt cgctcaagcg cgcatagatc
     1261 gcgtcgcggc caccgcgaag cgcgctctga acggccgtac cgatttcgtc tagcgtttct
     1321 tcgagcaggg aggctggcag gtcggtcatc ggccgttcgt taacgtgaaa cgccttgcgt
     1381 gccgaccacg accaccaccc tcgcatctac cggaggtggc ccgaggtcgg cgatgcggcg
     1441 ttagcccaac gtcgcggggc acgtgccctt cggggaccga cggactatat cagcggcgca
     1501 taggcccgtg aagccgtagc gccgccagtt cgttcaaaac agctcgtcat ccagccccat
     1561 gacaccggcg gcaccgccga cgatggcttc ggcaaggccg acggcctggg tcagcacgtg
     1621 atcaccgtag gcccgcgccg tgaccacctt ggcccgccag aacgggtcgg catcgccgcc
     1681 gacgcggtgg gcctcggccg ccgccgccgc gcggaacagt tgccagccgc cggcgacgat
     1741 gccgagcagc cgcaggaaag gtaccgagcc ggcgtgcgcg gcacggatat ccttggcgta
     1801 gttcgccagc agccagttgc ccgcagcctc gagcgcagcg atcgccgtcg ccagtcgcgc
     1861 cgcgattgcg ccgaagtcgt cgccgcccag tgccgcgagt tccttctgcg tcttctgcat
     1921 gtcggcgagc acggccttga aggtcgcgcc ctgctcccgc gccatcttgc ggccgatcag
     1981 gtcattggcc tggattgccg tcgtgccttc gtaaatggcc gtgatccgtg cgtcgcgcag
     2041 atactgggcg gcgccggttt cctcgatgta gcccatgccg ccgtgaacct gaatgccggt
     2101 cgaggcgatc tcgatcgccg tttcggtcga ccagccctta accaccggaa tcatcaggtc
     2161 ggcaaaggcc tgatggcgcg cacgggtcgc ctcgtcggcg ccgtgcctgg cgtgatcctg
     2221 cgcggccgcg acgacgtagg ccagcgcccg catcgcttcg acctgcgcgc gcatggtcat
     2281 cagcatgcgg cgcacatcgg gatggcggat gattgccacc ttgggaccgc cgcggacgcc
     2341 gacctcgggg ccctgcacgc gctccttggc gtagtcacgg gctcgctggt aggcgcgttc
     2401 ggcaatcgca agtccttcca tgccaaccgc gaagcgcgcc gcgttcatca tgatgaacat
     2461 gtactcaagg ccgcggttct cctcgccgac cagataaccc tcggcgccac cgccgagttc
     2521 gccgccgtcg ccgaaggcaa gtacggcggt cgggctcgcg tgaatgccga gtttgtgttc
     2581 gatcgaaacg cagtgcacat cgttgcgcgc gccaatcgca ccgtcgtcgc cgaccaggaa
     2641 cttgggaacg acgaacagcg agatgccctt gacaccttcg ggcgccgtcg gcgttcgacc
     2701 gagcacgaga tgaacaatgt tctcggtcat gtcgtgctcg ccgtaggtga tgaagatctt
     2761 ctgcccgtag atccggtagc tgccgtcggc ctgcggttcg gcacgtgtac gcacggcggc
     2821 aagatcggag ccggcctgcg gttcagtgag gttcatcgtc cccgaccagg tgccgtcgac
     2881 catcttcggc aggaacttgt ccttgagcgc atcggtcccc gcgatcatca cggcttcgat
     2941 cgcgccctgg gtcagcatcg ggcatagcga gaacgcgatg ttcgaggact tccacatctc
     3001 gttcaccgct gccgagacca acttgggcag gccctggccg ccgtacttcg aatcgggcga
     3061 cagcgcggtc cagccgctct cggcgaacag gcgataggcg tcgccaaatc caggcggcat
     3121 cgtcaccttg cggtcctccc acctggcgcc gacgcggtcg ccgacgacgt tgagcggcga
     3181 aatcacttcc tcggcgaagc gcgcggcctc gtcgaggatc gcgtcgatca gatcggggct
     3241 gacctcctcg ttgcccggca gcgccgcgac ggcatcgagc ccagccactt cgttcatcag
     3301 gaatcgcatc tcgcgcaggg gcgccacgta ctcggccatc gttgttctcc tccgttgtcg
     3361 ctaatgttgt ttgcgctggt gaaatcggcc tctgtgccgc agctcagtcg ccacgtctgg
     3421 cgaggtaaca gctggcgtcg aaactgctca gccactgcgg tcgatagaca accagcagcg
     3481 tgacgatcat gcccgacagc caggcttcgg caaaggccaa caggctaagc gccggcaacc
     3541 agacttccag cagcgtcgcc gcctcgcccc gtccgccggc aagtcccagc agtccggcca
     3601 gcgcgccacc gccggccagc gccagacccg cagcgaaaaa gccgttgccg aagatgtaga
     3661 cgaaaggatg cgccggcgca aagcgacgca ggatacggac gacgccccaa gtcagcagca
     3721 cagggaagcc gccagcaccg agaaaacgca gcgcccaatc gacgaccggc gtcggcgaga
     3781 caccaagcgt cagggaactc gccgcggcga ctaaaccgag gccgaccgtc gccagccacg
     3841 ggccgacgat caaggtcagc acggtcgccc cggcgaggtg aaggcgcaat cccgaatcga
     3901 gttcgaactg catctgccag agcaccagca gcgccgcgca ggccgcaccg aaggcattgc
     3961 ggagcccgct atcgcgaaat cgtgcccagg gcgcggcgaa caaggccagc gccaagagcg
     4021 cgatggcgat gcccgcggcg aacatggctc accccagttc ggcgagcagt tgcggcacga
     4081 cctcgaagag gtcgccgacc agtccgtagt cggcaacctg gaagatcggc gcctcgccgt
     4141 ccttgttgat ggcgacaatc accttgctgt ccttcatccc ggcaagatgc tggatcgcac
     4201 ccgagatgcc gacggcgatg tagagatcgg gagcgacgat cttgccggtc tgtccgacct
     4261 gatagtcgtt gggtacgtag cccgcgtcga ccgcagcccg cgaggcgccc agcgccgcgt
     4321 tgagcttgtc ggccagcggt tcgagcaacg aatggaactt gtcgccgctg ccgaggccgc
     4381 gaccgcccga aacgatcacc cgtgccgcgg tcagctcggg gcgctcggac accgtcaact
     4441 cgcggccgac gacgcggctc aggccgagat cgggcccggc ttcgatcgtt tccaccgccg
     4501 ccgatccgcc atcgccggca gcatcgaacg ccgtggtgcg aaccgtcacg accttcaccg
     4561 cgtcgctgct ctgcaccgtg gcgaggacgt tgccagcgta gatcgggcgg acgaaggtat
     4621 cgggtgcctg cacggcgacg atgtcggaaa gctgcgccac atcgagcagc gccgcgaccc
     4681 gcggcatcag gttcttgccg aaggtcgttg ccggcgcgac gacatggctg cacgaaacgg
     4741 ctagtttggc gaccagcggc gcgaggttct cggccaactg gtcggcgtag tgcggggcgt
     4801 ctgcgtgcac cactttcgcg acgccggcga gcgacgctgc agcgctggcg acggcagcgc
     4861 agtcggcgcc ggcgaccagc accgatacct cgccgccgag gctggcagcg gccgtcagcg
     4921 cgttgcgcgt ggccggcttg atcgacttgt tgtcgtgttc ggctatgagc aggatagtca
     4981 tcagagcacc ttcgcttcgt tacgcagttt ttcgacgagt tctcgcgcat cggcgacgcg
     5041 gatgccggcc ttgcgcttcg gtggctcggc gaccgacagc tgggtgatcc gcggcgcgac
     5101 gtcgacgccg agcgcctcgg gcgtcagcgt atcgagcggc tttttcttcg ccttcatgat
     5161 gttgggcagc gttgcataac gcggctcgtt gaggcgcagg tcggtggtca ccacggcggg
     5221 tagcttgatc tccagcgtct ccagaccgcc gtcgatttcg cgggtgaccg tcgccgcttc
     5281 gctggcgacc accagcttgg aggcgaaggt cgcctgcggc cagccgagca gcgcagcgag
     5341 catctgcccg gtctggttgg cgtcgtcgtc gatcgcctgc ttgccgcaga tcaccaactt
     5401 cggcgcctcc ttatcgacaa cggccttgag cagcttggcc acagccagcg gctgcaggtc
     5461 ggcgctgctc tcgatgagga tcgcccgatc ggcgccgagc gccattgccg tgcgcagcgt
     5521 ttcctggcag ttggccagtc cggccgagac ggcgacgatc tccgtcgcca ccccggcctc
     5581 cttcagccgc accgcttcct cgacggcgat ttcgtcaaag ggattcatcg acatcttgac
     5641 gttggcgatg tcgacacccg acccatcgct cttcggacgg accttgacgt tgtaatcgac
     5701 cacccgcttg accggaacca gaactttcac cgatagcctc cttgcctgtt ctctgttcgc
     5761 cggcaacgac agcggcccgt tgccgcgcat tcgctgtcgg aaaatctgcc ggattatcgc
     5821 cgcattgact tgcgcgcacg gcttcaacca atatacgcca ccgtatggca gcgcacatac
     5881 ggtagcgtat ggaaaagcgc ggcgtcaagt tggcgcaagt ccgtttgccg agttcaggaa
     5941 agccatggcc gttcgcaagg aagtcagctc actcgacccg cagcgctgga tcgacgccgc
     6001 catcgagatt ctcgtcggcg gtaatgtcga tgccatccgc gtcgaagtgc tcgccaagac
     6061 cctcggcgtc accaagggca gtttctactg gcactttcgc gatcgcagcg atctgctagc
     6121 gcgcctgctg gctttctggc gcgatggccg catcgccgac atcacgcggc acgccgccag
     6181 cccgccgggc cgcgagcgcg agcaggcgct gcgcctgatc gagctctaca gcgccaaccg
     6241 cagccgcaag ggaattgcca tcgaactggc gatccgcgac tgggcgcgca aggacgaaat
     6301 ggccgcgaag gccgtcgagg aagtcgacgt ggtccgcctc gatctcgccg gaagcctgct
     6361 cgccgccagc ggctttccgc cggccgaggc gcgcgcgcgc agcctgctgc tctacgccta
     6421 tgtcttcggc cagagcctga tgttctacga ccatttcgac gacgatctgc cggcgctcaa
     6481 gcgatgggtt gccgatcaca tcgtcaatcg gtgaaaatcg ccccccgcca cgacacgctc
     6541 agggaacctc gaatgaagtg cgaaaccttt ctgttcggat cgattgaaat cgctcccgaa
     6601 tccatcatcg aatttccgaa cggcctgccg ggattccccg acaaccagcg atttgcgctg
     6661 atccacgaag agaacgcggg tgactcgccg gcgagcttca cgctgcagtc gcttgacgac
     6721 cgcaacatcg cgctgc
//
LOCUS       DATDAA010000062         2038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_144199,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000062 DATDAA010000000
VERSION     DATDAA010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144199"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VIS73_02080"
     CDS             <1..721
                     /locus_tag="VIS73_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY5761975.1"
                     /translation="IQKNTTIPTKASQVFSTADDNQGAVTIHVLQGEREMSAGNKSLG
                     QFNLEGIPPAPRGMPQIEVTFDIDANGIMHVTAKDKATGKENKITIKANSGLSEDEIQ
                     RMVKDAELHAEEDKKAHELADARNQCDAMIHMVKKSLTEYGDKIEADEKAQIETALKE
                     AEEAIRGGDKEAIEAKSQALGTAAQKLGEKMYAEQQAAEGAAAGGGDAGGGASASGGK
                     SKKDEGDVVDAEFTEVNDKKV"
     gene            841..1977
                     /gene="dnaJ"
                     /locus_tag="VIS73_02085"
     CDS             841..1977
                     /gene="dnaJ"
                     /locus_tag="VIS73_02085"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY5761976.1"
                     /translation="MAKRDFYEVLGVNRDASDDEIKKAYRKLAMKHHPDRNPDNPKAE
                     EHFKEAKEAYEILSEPEKRAAYDQFGHAGVDQQAGMGPGAGGFSGGFGDAFGGIFDEI
                     FGGRGGGGGGGRSNVYRGADLRYNLEITLEQAAFGTETKIRIPTMEACDSCKGSGAKA
                     GTQPKTCPTCHGQGQVRLQQGFFSIQQTCPKCHGTGRFVAEPCGSCHGAGRVKQYKTL
                     AVKIPGGVDEGDRIRLAGEGEHGVNGGPAGDLYVQIHLKPHPVFTRDHNDLHCEMPVS
                     FTTAALGGEIEIPTLDGAAKLRIPAETQSGRAFRLRGKGIKGVRNQAYGDLMCHVVVE
                     TPVNLTERQRELLRELDELNSHDAERHNPRAKSWMDKVKDFFAG"
BASE COUNT          457 a          636 c          666 g          279 t
ORIGIN      
        1 gatccagaag aacacgacga tcccaaccaa ggcgtcgcag gtgttctcga ccgctgacga
       61 caatcagggc gcagtcacga tccacgtact gcagggcgag cgcgaaatgt ctgccggcaa
      121 caagagcctc ggccagttca accttgaggg catcccgccg gcaccgcgtg gcatgccgca
      181 gatcgaggtc accttcgaca ttgacgccaa cggcatcatg cacgtgacgg ccaaggacaa
      241 ggcgaccggc aaggaaaaca agatcaccat caaggcgaac tcgggtctgt ccgaggacga
      301 gatccagcgc atggtcaagg acgccgagct gcatgccgag gaagacaaga aggcgcacga
      361 actcgccgac gcccgcaacc agtgcgacgc gatgatccac atggtgaaga aatcgctcac
      421 cgagtacggc gacaagatcg aagccgacga aaaggcgcag atcgaaacgg cgctcaagga
      481 agccgaggaa gcgattcgcg gcggcgacaa ggaagccatc gaggcaaagt cgcaggctct
      541 gggcacggca gcgcagaagc tcggcgagaa gatgtacgcc gagcagcagg cggcagaagg
      601 cgccgcggct ggtggtggcg atgccggcgg cggcgcttcg gcctccggtg gcaagtcgaa
      661 gaaggacgaa ggcgacgtcg tcgacgccga gttcaccgag gtgaacgaca agaaggtctg
      721 atactcgcaa acccgaccag ggtgccggga atcgtgggaa cagcagtctc ttccgcgatg
      781 cccggccttc tcacgtcggg ccttttcagt tgttgagaat ggttggcggg aatccagaaa
      841 atggcaaaaa gagattttta cgaagtgctc ggcgtcaatc gcgatgcgtc cgacgacgag
      901 atcaagaagg cctaccgcaa gctggccatg aagcaccacc ccgaccgcaa tccggataac
      961 ccgaaggccg aggaacactt caaggaagcc aaggaagcct acgagatcct ctccgaaccg
     1021 gagaagcgcg ccgcctacga tcagttcggc cacgccggcg tcgaccagca ggccggcatg
     1081 gggcctgggg ctggcggctt cagcggcggt ttcggcgacg ccttcggcgg catctttgac
     1141 gagattttcg gtggccgtgg cggcggtggc ggcggtgggc gttcaaacgt ctatcgcggc
     1201 gccgacctgc gttacaacct cgagatcacg ctcgagcagg cggccttcgg caccgaaacc
     1261 aagatccgta ttccgacgat ggaggcctgc gacagctgca agggcagcgg cgccaaggcg
     1321 ggcacccagc cgaagacctg tcccacctgc cacggccagg gccaggttcg cctgcagcag
     1381 ggtttcttct cgattcagca gacctgcccg aaatgtcatg gcaccggccg cttcgtcgcc
     1441 gagccctgcg gcagctgtca cggtgccgga cgcgtcaagc agtacaagac gctggcggtg
     1501 aagatccccg gcggcgtcga cgagggcgat cgcatccgcc ttgccggcga aggcgagcac
     1561 ggcgtgaacg gcggacccgc cggcgacctc tatgtgcaga tccacctcaa gccgcaccca
     1621 gtattcacgc gcgaccacaa cgatctgcat tgcgagatgc cggtgagctt caccaccgcc
     1681 gcgctcggcg gcgaaataga gatcccgacg ctcgacggtg cagccaagct gcggatcccc
     1741 gcggaaacgc aaagtggccg cgctttccgc ctgcgcggaa agggtatcaa gggggttcgc
     1801 aaccaggcct acggcgacct gatgtgccac gtggtcgtcg aaacgccggt gaatctgacc
     1861 gaacggcaac gcgaactgct gcgcgaactc gacgaactga acagccacga cgccgagcgg
     1921 cacaacccgc gcgcgaagtc gtggatggac aaggtcaagg acttcttcgc gggctgagcg
     1981 gttccgcccg acctgctcag cccggcaggt agatgccggc tgcgcgcatc gcggcgat
//
LOCUS       DATDAA010000063         2147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_39609,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000063 DATDAA010000000
VERSION     DATDAA010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39609"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..610
                     /locus_tag="VIS73_02090"
     CDS             161..610
                     /locus_tag="VIS73_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEY5761977.1"
                     /translation="MNVWVDADACPRPVKDILFRAARRTGIPLTLIANQMLMVPASKH
                     IRAVQVEKGFDVADNEIVRRVEAGDLVITADIPLASEVIAKGGRVLTPRGEVYDAGSI
                     AAALSMRNFMDTLRSSGVETGGPSAFSASDQHAFARALDTMLAKRPR"
     gene            complement(645..875)
                     /locus_tag="VIS73_02095"
     CDS             complement(645..875)
                     /locus_tag="VIS73_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2789 domain-containing protein"
                     /protein_id="HEY5761978.1"
                     /translation="MEANLHTMSNLFAQLGLADDRESIDRFIAAHRPLGNAIALYRAP
                     FWTPAQRAFLKEEIIEDADWAGVIDELNERLH"
     gene            complement(1002..1283)
                     /locus_tag="VIS73_02100"
     CDS             complement(1002..1283)
                     /locus_tag="VIS73_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761979.1"
                     /translation="MKPISLLLLSITLSGAVHAALPGDAAEGQRLHDVNCLKCHDAGV
                     YAKERRQIRSLDALRERIGGCTHMAGADFSPEQSQSVLKYLNERFYRLP"
     gene            complement(1386..>2147)
                     /locus_tag="VIS73_02105"
     CDS             complement(1386..>2147)
                     /locus_tag="VIS73_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY5761980.1"
                     /translation="IGAMDVERRTQRARRLGQHGLATVSLVGYTNAGKSTLMRALTGS
                     EVLVANKLFATLDTTVRALHPESVPRVLVTDTVGFIKNLPHALVASFKSTLDEALDAA
                     LLLHVIDASDPGFERQLAVTEAVLHDIGAGAVPRLLVFNKIDNTGDPATQAGHAATLR
                     VRYPDCIVMSARRPDEVAQLRRTILAFFQRERVEAELFLPWAQQQLRGAIYAQCEVLD
                     ERSTDEGSILRVRGDSEAVAALRGQHGPTPSAACA"
BASE COUNT          328 a          713 c          742 g          364 t
ORIGIN      
        1 cggcagctgg actcggccgg gcgaagtgat tcgggccaga ccgtcgcgcc ggcaacgcac
       61 aatccctttg ccggcctcgg cggcctgctc aagaaaccgt agtgaccagc tcaggggcgc
      121 cgacgcccgc cgttttgcca gcgacacccg gaaggcgcgg gtgaacgtct gggtcgacgc
      181 cgacgcctgc ccacggccgg tgaaggacat tctcttccgt gccgccaggc gaaccggaat
      241 tccgctgacc ttgatcgcca accagatgct catggtgccg gcatcgaagc acatccgtgc
      301 cgtgcaggtg gaaaagggct tcgatgtcgc cgacaacgag atcgtccgcc gcgtcgaagc
      361 cggcgatctg gtcatcaccg ccgacattcc gctggccagc gaggtcatcg ccaagggcgg
      421 ccgcgtgctc acgccgcgcg gcgaggtcta cgacgccggg agcatcgccg cggcgctgtc
      481 gatgcgcaat ttcatggaca cgctgcgttc gtcgggtgtc gaaaccggcg ggccgtcggc
      541 tttctcggcc agcgaccagc acgcctttgc ccgtgcgctc gacacgatgc tggcgaaacg
      601 cccgcgttag cctggtctcg ggcccgccta ccggcgccca gacttcagtg caatcgttcg
      661 ttgagttcgt cgatcacccc ggcccagtcg gcgtcttcga tgatttcttc cttgaggaag
      721 gcgcgctgcg ccggcgtcca gaacggcgcg cgataaaggg cgatcgcgtt gcctagcggg
      781 cggtgggcgg caatgaagcg gtcgatcgat tcacggtcgt cggccaggcc gagctgggca
      841 aacaggttcg acatcgtgtg caggttcgct tccatggtct tgcctttcgc ggcaaaggcg
      901 cggtgcgccg gtcctgattc gatggaagcg atgcgcgcga gttccgggac cctgctgtca
      961 gcgagcgttc gctagtttcc gtgctcgtcg ccccgaagtc cttacggtag tcggtagaag
     1021 cgctcgttga ggtacttcag tacgctttgc gactgctcgg gcgagaagtc ggcgccggcc
     1081 atgtgggtgc agccgccgat gcgctcgcgc agcgcatcga gcgagcgaat ctgccgccgc
     1141 tccttggcat agacgcccgc gtcatggcac ttcaggcagt tgacgtcgtg cagacgctgg
     1201 ccttcggcgg catcgccggg gagcgcggcg tgaaccgcac cggacagcgt gatcgacagc
     1261 agcaggagcg aaatcggttt catgattgag tcctccggag cgcgcgatac ggtgcgccca
     1321 gcatacgcct gcgcgccgtg cagtcggggt tccgcgtctc gtggtgccaa aggggtcaac
     1381 agcgctcagg cgcaggcggc cgagggcgtc gggccgtgtt ggccgcgcag cgctgccacc
     1441 gcctcgctgt cgccgcgcac gcgcaggatg ctgccctcgt ctgtgctgcg ctcgtcgagc
     1501 acctcgcact gcgcgtagat cgcgccgcgc agctgctgct gcgcccaggg caggaacagc
     1561 tcggcctcga cgcgctcgcg ctggaagaag gcgaggatgg tccggcgcaa ctgcgccacc
     1621 tcgtcggggc ggcgcgcgct catcacgatg cagtccgggt agcggacgcg cagcgtcgcc
     1681 gcgtgtcccg cctgcgtcgc cgggtcgccg gtgttgtcga tcttgttgaa gacgagcagg
     1741 cgcggcaccg cgccggcacc gatgtcgtgc agcaccgctt cggtcaccgc gagctgccgc
     1801 tcgaaaccgg ggtcgcttgc atcgatgaca tgcagcagca gcgcggcatc gagggcctcg
     1861 tcgagtgtcg acttgaacga ggcgaccagc gcgtgcggca ggttcttgat gaagcccacg
     1921 gtgtcggtca ccagcacgcg tggaacgctc tccgggtgga gcgcacgcac cgtcgtgtcg
     1981 agcgttgcga acagcttgtt ggcgaccagc acttcgctgc cggtgagtgc gcgcatcagc
     2041 gtggacttgc cggcgttggt gtaaccgacc agcgaaaccg tggcgagacc atgctgcccc
     2101 agccggcgcg cccgctgggt cctgcgctcg acgtccatcg ccccgat
//
LOCUS       DATDAA010000064         2317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_459200,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000064 DATDAA010000000
VERSION     DATDAA010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459200"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2046
                     /locus_tag="VIS73_02110"
     CDS             <1..2046
                     /locus_tag="VIS73_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY5761981.1"
                     /translation="TAPLSAAESDGNSRPALQAAPIVVTATRSAQSSFDLPVSIDVVE
                     GAQITEAQPLLNAAEALARVPGLVAPNQYRLSSDQQLSSRGFGSRAAFGVRGIRIYSD
                     GIPQTMPDGQGQPGTFNLATAERMEVMRGPFSALYGNSAGGVVQIFSRDAAPEPTLSA
                     SYYAGDFDTWRAGLQFGGQFGAVNALVDGSRYQTDGFRQHSAAQRDQLSARLVMRASD
                     ADKITFVASDLDQPYNEDPQGLTRVQMEADPTGTQTTSTTFNTGGSKAQTHLGMNWEH
                     RVDERNDFAAMVYGGQRDSLQRLGFVGGGALQSGGVSVIARDFSGVDLRWRHVAPLAS
                     GALTVTAGLNFDTMEDVRQGYENNSGVLGLIKRDEVNVARNFDQYVQAEWLAGADWVF
                     SGGLRHSEIRLESEDRYIVGANPDDSGSVKFTSTNPVVGVLYHLTPAVNLYANAGRGF
                     ETPTLIEIAYRSDGGSGSNFALRPSESTHVEAGVKAFIGGTRVNAAVFRAETSDEIVV
                     DTNTGGRATYKNAGDTSRQGIEVSVGGEIAGGLSAYASYAYLDAQFDDSFLSNGSTVA
                     SGNKIPGAPESTAYAELAWRLPAIGLFTAVEANYIGRTYVNDLNADSVEPSTVVNWRL
                     SFTQQLGRFTLSEFARVENLFDEDYVGGVLVNAGFNRYFAPAPGRNALLGVSLAARF"
     gene            2072..>2317
                     /locus_tag="VIS73_02115"
     CDS             2072..>2317
                     /locus_tag="VIS73_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761982.1"
                     /translation="MLRSLMAALLAVAAGGAGAFDYTGEKRVTLHARDGTSLVIGRLG
                     FKAEGEVSRFAFDIDTRVMHDHFLSMKEFKCAEGSAEL"
BASE COUNT          391 a          775 c          758 g          393 t
ORIGIN      
        1 acggcgccgc tgagcgctgc ggaatccgac ggtaattcgc gtccggcact gcaggccgcg
       61 cccatcgttg tcacggcgac gcgcagcgcg cagtcgagct tcgacctgcc ggtttcgatc
      121 gacgtcgtcg agggtgcgca gattaccgag gctcagccgc tgctcaacgc cgccgaggcg
      181 ctggcgcggg tacccggcct cgtcgcgccc aaccagtacc gcctgtcgtc ggaccagcaa
      241 ttgtcgtcac gtggtttcgg ttcgcgcgcc gccttcggcg tacgcggaat ccgcatctac
      301 tccgacggga ttccgcagac catgcccgat ggccagggcc agcctggcac cttcaacctc
      361 gccacggccg agcgaatgga agtcatgcgt ggccccttct cggcgctcta cggcaactcc
      421 gccggcggcg tcgtgcagat cttctcgcgc gatgcggcgc ccgagccgac gctttctgcc
      481 agctactatg caggcgactt cgatacctgg cgcgccggcc tgcagttcgg cggccagttc
      541 ggtgccgtca acgcgctggt cgacggatca cgttaccaga ccgatggttt ccgccagcac
      601 agcgccgcgc agcgcgacca gctcagcgcc cgactggtca tgcgtgccag tgatgcggac
      661 aagatcacct tcgttgccag cgatctcgac cagccctata acgaagaccc gcagggcctg
      721 acgcgggtgc agatggaggc cgacccgacc ggaacgcaaa ccacgtcgac gaccttcaac
      781 accggcggca gcaaggcgca gacgcacctg ggaatgaact gggagcatcg cgtcgacgag
      841 cgcaacgatt tcgcggccat ggtctacggc ggccagcgcg actcgctgca gcgtctcggt
      901 ttcgtcggcg gcggcgcgct gcagtcgggc ggggtcagcg tcatcgcccg cgatttttcc
      961 ggtgtcgatc tgcgctggcg gcacgtggcg ccactcgcca gcggcgcgct gacggtcacc
     1021 gccggcctca acttcgacac gatggaagat gtacgccagg gctacgagaa caacagcggc
     1081 gtgctcggcc tgatcaagcg cgacgaagtg aacgtcgcgc gcaacttcga ccagtacgtc
     1141 caggccgaat ggctggcggg tgctgactgg gtcttcagtg gcggcctgcg tcacagcgaa
     1201 atacggctcg agtcggagga tcgctacatc gtcggcgcca acccagacga cagcggctca
     1261 gtcaagttca ccagcaccaa ccccgtggtc ggcgtgctct accacctgac cccggcggtc
     1321 aatctctacg ccaacgccgg ccgcggcttc gaaacgccga cgctgattga gatcgcctat
     1381 cgttccgacg gcggctcggg atcgaatttc gcgctgcggc ccagcgaatc gacgcacgtc
     1441 gaagccggtg tcaaggcatt tatcggcggc acgcgggtca atgcggcggt gttccgtgcc
     1501 gagacctcgg atgaaatcgt cgtcgacacc aataccggtg gtcgtgccac ctacaagaat
     1561 gccggcgata cgtcacgcca gggaatcgag gtttcggttg gcggcgaaat tgccggcggc
     1621 ctgagcgcct acgcgtccta cgcctacctc gacgcgcagt ttgacgacag ctttctttcc
     1681 aacggcagca cggttgcctc cggcaacaag attccgggtg cgcctgagag cacggcatac
     1741 gccgaactcg cctggcgcct gccggcgatc ggcctgttca cggcggtgga ggccaactac
     1801 atcggccgca cctacgtcaa cgacctcaac gccgattcgg tcgagccgtc gacggtggtc
     1861 aactggcggc tgtcgttcac gcagcagctg ggccgcttca cgctgagcga gttcgcgcgc
     1921 gtcgagaacc tgttcgacga ggactacgtc ggtggcgtcc tcgtgaacgc cggtttcaac
     1981 cgctacttcg cgccggcacc ggggcgcaac gcgctgcttg gcgtgtcgct ggcggctagg
     2041 ttctgaggcg atgcgggcga gcggcgctgt catgcttcgc agcctgatgg cggcgctgct
     2101 tgccgttgcc gccggtggtg ccggcgcctt tgactacacc ggcgaaaaac gggtgacgct
     2161 gcatgcgcgg gatggcactt cgctggtcat tggccgcctc ggcttcaagg cggaaggcga
     2221 ggtcagccgc ttcgcgttcg acatcgacac gcgggtgatg cacgaccact tcctgtcgat
     2281 gaaggagttc aagtgcgccg aggggagcgc cgagctg
//
LOCUS       DATDAA010000065         2310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_74533,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000065 DATDAA010000000
VERSION     DATDAA010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74533"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VIS73_02120"
     CDS             <1..1218
                     /locus_tag="VIS73_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761983.1"
                     /translation="LFTIASALAVFYIVGDLGGITTIMEQLAVVDGKEDLMSWHGIVG
                     PGTEWPTATDYLIWMLAIDASWALVYCVSPWQSSRHLMAKNEHVVLRAAILACLFVGL
                     LQVMVYGMGGIINLGDPNIDPPESATIWASLTMMPGILGALMLAGIMAAVLSSASTFL
                     SLVGFSASNDIGIHATGDEARTLRFSRLMMLLIGSIALVAALIFPPNIFWLTTFIATV
                     FASSWGPVGLMSIWSKRITESAAFWGMLAGLVFNVVPKFFEFIGMVHFPSYMNPVIIG
                     AFASLVVTIVVSRRTSVTPEESAYLDKLHQTPAEEVSEKRMRLTLTAPIVVLVLNGLI
                     MPVLLLVYYVYPFQKASGTLTPDGSLNWFTGEAMLAVSWIFVYVAVSIFAIRVIRRYY
                     DPSSPYVEHGSVG"
     gene            complement(1207..1791)
                     /locus_tag="VIS73_02125"
     CDS             complement(1207..1791)
                     /locus_tag="VIS73_02125"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY5761984.1"
                     /translation="MKKISGTVVAVNAGTNDDLSKNAADGIQVELDGIVGDTHRGYVR
                     KCWQGDKQAKGTSRRNERQWSAMSVEEIAEISEALDLVTPLDAARLGANLCIAGVSQL
                     SRLPRGSLLRFPSGAELMVEEYNPPCLGMGRQIAGSYATRSGKVLSDTDFSKAAKLTR
                     GVVGVVEAAGSINAGDEVLIEIYETPSWLERSAD"
     gene            1873..2277
                     /locus_tag="VIS73_02130"
     CDS             1873..2277
                     /locus_tag="VIS73_02130"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY5761985.1"
                     /translation="MINGRCECGEVRFEVRGEILDFSHCHCSICRRLHGAAYASFAGV
                     ERSAFRFIAGETSITTYPSSARNDRVFCSKCGSAVLCDPKTEPGRLYLSMSAIEGNPP
                     LPEAYHQYAASRAPWHDIDDELKRYDAAVPPG"
BASE COUNT          381 a          747 c          721 g          461 t
ORIGIN      
        1 ctgttcacca ttgcaagcgc cctggccgtc ttttacatcg tcggcgatct cggcggcatc
       61 acgacgatca tggagcagct ggccgtggtc gacggcaagg aagacctgat gtcctggcac
      121 ggcatcgtcg gccccggcac ggagtggccg acggcgacgg actacctgat ctggatgctg
      181 gccatcgacg ccagctgggc gctggtctac tgcgtcagcc cgtggcaatc gagccgccac
      241 ctgatggcga aaaacgagca cgtcgtgctg cgcgccgcca tcctcgcctg cctgttcgtc
      301 ggcctgctgc aggtcatggt ctacggcatg ggcggcatca tcaatctcgg cgacccgaat
      361 atcgatccgc cggagagcgc cacgatctgg gcgtcgctga cgatgatgcc cggcatcctg
      421 ggggcgctga tgctcgccgg catcatggcg gcggtgctgt cgtcggcgtc gaccttcctg
      481 tcgctggtcg gattcagcgc cagtaacgac atcggcattc atgcgacggg cgacgaggcg
      541 aggacgctgc gcttcagccg cctgatgatg ctgctcatcg gttccatcgc attggtggcg
      601 gccctgattt ttccgcccaa catattctgg ctgacgacgt tcatcgccac ggtgttcgcc
      661 tcgtcctggg gtccggtcgg gctgatgagc atctggagca agcgcatcac ggagtccgcg
      721 gctttctggg gaatgcttgc cgggctggtc ttcaacgtgg tgccgaagtt cttcgaattc
      781 atcggcatgg tccacttccc gtcctacatg aacccggtca tcatcggcgc cttcgccagc
      841 ctggtagtca cgatcgtcgt gtcgcgccga acctcggtga cacccgagga atccgcctac
      901 ctggacaagc tgcaccagac accggccgag gaagtcagcg aaaagcgcat gcgcctgacg
      961 ctgaccgctc ccattgtcgt gctggtcctg aacggcctga tcatgcccgt gctactgctg
     1021 gtctattacg tttacccgtt ccagaaagcc agcggcacgc tgacacccga cggatcgctg
     1081 aactggttca ccggggaggc catgctcgcg gtgagctgga tattcgtcta cgtcgctgta
     1141 tcgattttcg ccatcagggt gattcgccgc tactacgacc cttcgtcacc gtatgtcgag
     1201 cacgggtcag tcggctgatc gctcgagcca gcttggcgtc tcgtagattt cgatgagcac
     1261 ctcgtcgccg gcattgatgc ttcccgcggc ctccacgacc ccgacgacgc cgcgggtcag
     1321 cttggcggct ttcgagaagt cggtatcgga cagcaccttg cccgaccggg tcgcgtaact
     1381 cccggcgatc tgcctgccca tgccgagaca gggcgggttg tattcctcca ccatgagttc
     1441 cgcgccggac ggaaacctga gcaggctgcc tctgggcagg cgcgacagct gcgacacgcc
     1501 cgcgatgcag agattcgcgc ccagccgggc cgcgtcgagc ggcgtgacga gatcgagggc
     1561 ctcgctgatc tcggcaattt cctcgaccga catcgccgac cactggcgct cgtttctgcg
     1621 cgaggtgccc ttggcctgct tgtcgccctg ccagcacttg cgcacgtagc cgcgatgcgt
     1681 atcgccgacg atcccgtcca gttcgacctg gatgccgtcg gccgcgttct tgctcaaatc
     1741 gtcgttggtg ccggcgttta cggcgacgac ggtgccgctg atttttttca aggctggatc
     1801 tccgggctgg actatacgtg tgactgcgcg cgatgatgat aacgtttccc ggggctgcgg
     1861 tgggagagat caatgatcaa cggacgttgt gaatgcggcg aggtgcgctt cgaagtgcgc
     1921 ggcgaaatcc tggacttcag tcactgccat tgttcgatct gccggcggct gcacggcgcg
     1981 gcctacgcct cgttcgcagg cgtcgagcgc agcgcgtttc gctttatcgc cggcgagaca
     2041 agcatcacga catacccctc ttcggcgagg aacgaccggg ttttctgctc gaaatgcggc
     2101 tccgcggtat tgtgcgaccc caaaaccgag cccgggcgcc tgtacctcag catgagcgcg
     2161 atcgagggta atccgccgct gcccgaggca tatcaccagt acgcagcctc gagggcgccc
     2221 tggcacgaca tcgacgacga actgaaacgc tacgacgctg ccgtgccgcc gggctgagag
     2281 gcatggcgca ccagtcgcgc aaactcgcgg
//
LOCUS       DATDAA010000066         2346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_284374,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000066 DATDAA010000000
VERSION     DATDAA010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284374"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VIS73_02135"
     CDS             <1..533
                     /locus_tag="VIS73_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761986.1"
                     /translation="ASPTTPAPSIDRPAETKAVPARPRPTEERRAAESIPVPPPASVE
                     SRRPAPLVPRREPIAQIRPGDDVVELAAIHGFDPVPVPVPDNTRFSRLRLGMTKQDAE
                     ALLGRPDTSLHYANEGARIPFYASDDSSRWETFYRGQGRLLFSGAWLTGEVRLIRIEY
                     DPGEDGRPDPDHPHRR"
     gene            569..1738
                     /locus_tag="VIS73_02140"
     CDS             569..1738
                     /locus_tag="VIS73_02140"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY5761987.1"
                     /translation="MNFKKLTELDVRGKKVFIRADLNVPQDDAGNITDDTRIKAALPG
                     IRYVLDNGGLVMVTSHLGRPTEGEFKPADSLAPIGKRMGELLGKSVRLVQDWVDGGFE
                     VKPGEVVLLENCRCNKGEKKNNDELAKKMAALCDIWVHDAFGTAHRAEATTHGMGKFA
                     RIACAGPCVAAELEALTKALAAPKRPLMAIVGGSKVSTKLTVLDALSTKVDQLVVGGG
                     IANTFLLAAGKKIGKSLAEHDLVDQAKAVMAKVNVPLPTDVIVATEFKADARARVKSV
                     DEVADDEMILDIGPASAKALAALVNAAGTIVWNGPVGVFEMEAFAGGTKTLGHAIGTS
                     KAFTLAGGGDTVAAINAFGLESKVGYISTAGGAFLEFLEGKTLPAVEILGQRADG"
     gene            1865..>2346
                     /locus_tag="VIS73_02145"
     CDS             1865..>2346
                     /locus_tag="VIS73_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEY5761988.1"
                     /translation="MLLNCIVYQRGAKLADIPVADISNYVGRHDCFVWVALRDADDAE
                     LLQMQEEFGLHELAVEDARHGHQRPKIEEYGDSIFAVVHTLELIAGEISVGEVNIFVG
                     ANYVLSVRNRSTRTLLGVRERCEREPHLLTHGSGFVFYALMDAVVDRYFPLIDELETE
                     L"
BASE COUNT          443 a          820 c          744 g          339 t
ORIGIN      
        1 cggcaagccc tacaacgccc gccccgagca tcgaccgccc ggccgaaacc aaggcggtgc
       61 cggcgcgacc acgccctact gaggaacggc gcgccgccga atcgattccg gtgccgccgc
      121 cggcaagcgt cgaatcgcgc cgcccggcgc cgctggtgcc gagacgcgag ccgatcgcgc
      181 agatccgtcc cggcgacgac gtcgtcgaac tcgcggcgat tcacggcttc gacccggtgc
      241 cggtgccggt tcccgacaac acgcgcttct cgcgcctgcg gctgggcatg accaagcagg
      301 atgccgaggc gctgctcgga cgccccgaca cctcgctgca ctacgccaac gagggcgcgc
      361 ggattccctt ctacgccagc gacgacagtt cgcgctggga gaccttctac cgtggccagg
      421 gccggctgct gtttagtggc gcctggctca ccggcgaagt gcgcctgatc cgcatcgagt
      481 acgaccccgg cgaagacggc cggcccgatc ccgatcaccc gcatcgccgg taagcgaatt
      541 cgatttcgac aacaccaaag gaacccacat gaacttcaaa aagctgaccg agctcgacgt
      601 ccgcggcaag aaagtcttca tccgcgccga cctcaacgtg ccgcaggacg acgccggcaa
      661 catcaccgac gacacgcgaa tcaaggccgc gctgcccggc atccgctatg tgctggacaa
      721 cggcggtctg gtgatggtca cctcgcacct tggccgaccc accgaaggcg agttcaagcc
      781 cgcggattcg ctggcgccga tcggcaagcg catgggcgaa ctgctcggca agtcggtgcg
      841 cctcgtgcag gactgggtcg acggcggctt cgaagtaaaa cccggcgagg tcgtgctgct
      901 ggaaaactgc cgatgcaaca agggcgaaaa gaagaacaac gacgaactcg cgaagaaaat
      961 ggcggcgctc tgcgacattt gggtacacga cgccttcggc accgcccacc gcgccgaggc
     1021 aaccacgcac ggcatgggca agttcgccag gatcgcctgc gccggcccct gtgtcgccgc
     1081 cgaactggaa gcgctgacca aggcactggc cgcaccgaag cgcccgctga tggcgatcgt
     1141 cggcggctcg aaggtctcga ccaagctcac cgtcctcgat gcgctgtcga ccaaggtcga
     1201 ccagctggtg gtcggcggcg gcatcgccaa taccttcctg ctcgccgccg gcaagaagat
     1261 cggcaagagc ctcgccgagc acgatctcgt cgatcaggcg aaggcggtga tggccaaggt
     1321 caatgtgccg ctgcccaccg acgtgatcgt cgccaccgag ttcaaggccg acgcgcgggc
     1381 gcgcgtgaag tcggtcgacg aggtcgccga cgacgagatg atcctcgaca tcggccccgc
     1441 ctcggcgaag gcgctggcgg cgctggtcaa cgcggccggc accatcgtct ggaacgggcc
     1501 cgtcggcgtc ttcgaaatgg aagcctttgc cggcggcacc aagacgctgg gccacgcgat
     1561 cggcacctca aaggccttca cgctggccgg cggcggcgat accgtcgcag cgatcaacgc
     1621 cttcggcctc gagagcaagg tcggctacat ctccaccgcc ggcggcgcct tcctcgagtt
     1681 cctcgaaggc aagacgctgc cggccgtcga aattctcggc caacgcgccg acggctgacc
     1741 cccggcttaa tcgtgacgaa cgggcggctg cagccgcccg tttgcgttgg cgcccagccg
     1801 ccttgcccga catggtcacg cggcggacaa tcgccatcaa acgccaagga aagaagccga
     1861 ccgcatgctc ctcaactgta tcgtctacca aagaggcgcc aagctcgccg acattccggt
     1921 cgccgacatc agcaactacg ttggccgcca cgactgcttc gtctgggtcg cgctgcgcga
     1981 cgccgacgac gccgaactgc tgcagatgca ggaggagttc ggcctgcacg agctggccgt
     2041 cgaggacgcg cgacacggcc accagcggcc gaagatagag gagtacggcg attcgatctt
     2101 cgccgtcgtg cacacgctgg agctgatagc cggcgagata tcggtcggcg aggtcaatat
     2161 cttcgtcggc gccaattacg tgctctcggt gcgcaaccgc tcgacccgga cgctgctcgg
     2221 ggtacgcgag cgctgtgagc gcgagccgca cttgctgacg cacggctcgg gattcgtctt
     2281 ctacgcgttg atggacgccg tcgtcgaccg ctacttcccg ctgatcgacg aactcgaaac
     2341 cgagct
//
LOCUS       DATDAA010000067         7318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_354361,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000067 DATDAA010000000
VERSION     DATDAA010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354361"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="VIS73_02150"
     CDS             <1..1375
                     /locus_tag="VIS73_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761989.1"
                     /translation="IPDAEAALTMLREIDATQPELPRLESLLRLLQINAAMREAYDSA
                     AQAAAWKSLETLAAEGPLADLRPEGDFALSELRSSQLVRAGGALAADRLGECREALLQ
                     ARMLARVLGDVAVPPEENRFVDRLQRAADAAAARGESALEYGYRMVMHEMQPDYPMLR
                     LTLQEAAERAREAALAPVAIVTAGDSEAAQIVAVALADRLSARIAARMPHDLRLVSIR
                     PLLPPMPDPATAAAPPAASVAATGEAQWEMLLDVADAGVEIETTEQMQRQRVQTEIVP
                     IANPEHAAWAALPASERGTTPEPPATIGQPIVENVMIGVTRQRQRARLVMRYHLLETT
                     PTGDLVGDSISLDRVVEGESHGAVEIGAFVLPARRADLPTPEQMVAALVDEAVVAIGE
                     RLETLFGDNVARQTALAERAAVEATPLLEIEHRAAALAVAEAKGSATAELRSALRERV
                     LARRIAN"
     gene            1386..1616
                     /locus_tag="VIS73_02155"
     CDS             1386..1616
                     /locus_tag="VIS73_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEY5761990.1"
                     /translation="MTHRSAPAAREVRCPECGRASLWSPDNPYRPFCSARCKQIDLGA
                     WASESYRVPVVERDDSELPDLPTESADDSGRN"
     gene            complement(1657..2616)
                     /locus_tag="VIS73_02160"
     CDS             complement(1657..2616)
                     /locus_tag="VIS73_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HEY5761991.1"
                     /translation="MSGTPRPVTEVAAAVLLRGADNDLREEYLLAQRPPGKVYAGYWE
                     FPGGKVEAGESPHDALVRELTEELGITVADATPWLVREFDYPHARVRLHFFRIRRWYG
                     TIAPIEHSGFAWCRVHQAPSVAPVLPANTQIMAALDLPTRYAISCAEEHGVEEELERL
                     QRALAGGLRLLQIRDKTLPEPQRALFARRAIDLARPAGARVLVNSDVQLAADIAADGV
                     HLASTELMRRAARPPFALVGASCHSPAELAQAVSLGLDYALLGPVLPTRSHADSVPLG
                     WSEFAALASGQPLPVYALGGMRNGLLDEAMRHGAHGIALMRGW"
     gene            complement(2613..3551)
                     /locus_tag="VIS73_02165"
     CDS             complement(2613..3551)
                     /locus_tag="VIS73_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5761992.1"
                     /translation="MPADRAKKPPHAAPDSLSLARLLARAEALAERLEAVLPPPPEAV
                     DWECAIAFRWRRVGSRGVLQAIAHPHPIRLEDLRDIDEQKDRLVANTRQFVACRPANN
                     ALLTGARGSGKSSLVKALLHAFADRGLRMIEVDRHDLADLPDLIALVDGRPERFIVFC
                     DDLSFEAGDASFRELKVALDGSLAAPPDNLLIYATSNRRHLMPEFFAENLETQRHGGE
                     IHPGESTEEKTSLSERFGLWLSFYPFDQDAYLDIVAYWLQHYGANAKQVEVARQEALN
                     WALGRGARNGRVGWQFARDWAGRQERLPRRPKRAGR"
     gene            3980..5116
                     /locus_tag="VIS73_02170"
     CDS             3980..5116
                     /locus_tag="VIS73_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEY5761993.1"
                     /translation="MSNGSAAAGAKALPTAVTAPASGGAAPPSVVAQTSAVGGSASPN
                     GNVAAGNGTAPPAYDWGAACTAARQQGDVKPSAAVLRDFFEHHFVPWSLTNPDGTQEG
                     TVTGYYEPLLRGSRKREGAFTVPIHAVPDDLLTIEMGDLFPELKGKRVRGRVEGKRVV
                     PYYDRGGIRARAAELREKVLVWGDDAVEVFFLQIQGSGRVRLPDGSLMRIGYADQNGH
                     PYNSIGRLLVERGDLLLEQASMQGIQAWARANPGKLEALLDANPSYVFFRELPAGDQG
                     PIGALGVPLTAGRSIAVDPAFVPLGAPVFLATTEPNSAVPLNRLMFAQDTGGAIKGVV
                     RADFFWGFGPEAGALAGRMRQIGRMWVLLPRTAVASDLPPIARR"
     gene            5287..6489
                     /locus_tag="VIS73_02175"
     CDS             5287..6489
                     /locus_tag="VIS73_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY5761994.1"
                     /translation="MDALKSGSDTLFVLLGAIMVLAMHAGFAFLEVGTVRRKNQVNAL
                     VKIIADFAMSTIAYFFVGYSVAYGVSFFSGAETLAAKNGYELTKFFFLLTFAAAIPAI
                     ISGGIAERARFNPQLAATFVLVALVYPFFEGIVWGGAYGVQEWLAATFGASFHDFAGS
                     VVVHAMGGWLALPAVLLLGARRGRYGKDGTVSAHPPSSIPFLALGAWILTVGWFGFNV
                     MSAQAIEGISGLVAVNSLMAMVGGTLAALAAGKNDPGFVHNGPLAGLVAVCAGSDLMH
                     PLGALIVGGVAGGLFVLMFTLTQNRWKIDDVLGVWPLHGLCGAWGGIAAGIFGLQALG
                     GMGGVSFMAQLIGTLMGVGVALVGGFVVYGTIKQIMGLRLDAEDEFNGADLAIHRISS
                     TAERETLW"
BASE COUNT         1102 a         2478 c         2494 g         1244 t
ORIGIN      
        1 cattcccgat gccgaggcag cgctgacgat gctgcgcgag atcgacgcga cgcagcccga
       61 gttgccacga ctcgaatcgc tcttgcgcct gctgcagatc aacgccgcca tgcgcgaagc
      121 ctacgattcg gcggcgcagg cagcggcctg gaaatcactc gaaacgctgg ccgccgaggg
      181 gccgctcgcc gatctgcgcc ccgaaggcga ttttgcgctg agcgaattgc gctcttctca
      241 gctggtccga gccggcggcg cactcgctgc cgatcgcctt ggcgagtgcc gcgaagccct
      301 gttgcaggcg cggatgctcg ctcgggtgct cggcgacgtc gccgtgccgc ctgaagagaa
      361 ccgcttcgtc gaccggctgc agcgcgccgc cgatgccgcc gccgcgcgtg gcgaatcggc
      421 gctcgaatat ggctaccgga tggtcatgca cgagatgcag cccgattatc cgatgctgcg
      481 cctgaccctg caggaggccg ccgagcgcgc ccgcgaagcg gcgctggcgc cggttgccat
      541 cgtcaccgcc ggcgattcag aagcggcgca gatcgtcgcc gtcgcgcttg ccgaccgcct
      601 ctcggcgcgg atcgctgcgc gcatgcctca cgacctgcgc ctggtgtcga tccgtccgct
      661 gctgccgccg atgcccgatc cggcgacggc cgcggcgccg ccggccgcgt cggtcgccgc
      721 aaccggcgag gcgcagtggg agatgctgct cgacgtggcc gatgccggtg tcgagatcga
      781 aacgaccgag cagatgcagc ggcagcgcgt gcagaccgag atcgtgccta tcgccaatcc
      841 cgagcacgcc gcatgggccg ccttgccggc gagcgagcgc ggcaccacgc ccgagccgcc
      901 ggcgaccatc ggccagccga tcgtcgagaa cgtcatgatc ggcgtcaccc gccagcgcca
      961 gcgggcccgc ttggtgatgc gctatcacct gctcgagacc acgccgaccg gcgatctggt
     1021 cggcgactcg atctcgctcg accgtgtcgt cgagggcgag agccacggcg ccgtcgagat
     1081 tggcgccttc gtcttgccgg cgcgccgcgc cgacctgccg acgcctgagc agatggtcgc
     1141 cgcgctggtc gatgaggcag tggttgcgat cggcgaacgg ctggagacgc tgttcggcga
     1201 caacgtcgcg cgccagaccg cgctcgccga gcgtgccgcc gtcgaggcga cacccctact
     1261 cgagattgag caccgcgccg cggcgctggc cgttgccgaa gccaagggca gcgcgacggc
     1321 cgaactgcgc agcgccttgc gcgagcgggt cctcgcccgc cgcatcgcca attgagggtg
     1381 ggcgcgtgac gcaccgatcg gcgccggccg cacgtgaagt ccgctgcccc gagtgcggcc
     1441 gtgccagcct ctggtcgccc gacaatccgt atcgaccgtt ctgcagcgcc cgctgcaagc
     1501 agatcgacct cggcgcctgg gcgtcggaga gttaccgtgt gccggtcgtt gagcgcgacg
     1561 acagcgagct gcccgacctg ccgaccgagt cggcagacga ttccggccgc aactgagtaa
     1621 gcgccggcag gccgccgcga ccgagccggt gccgccttac cagccgcgca tcagcgcgat
     1681 tccgtgcgcg ccgtggcgca tcgcttcgtc gagcaggccg ttgcgcatgc cgcccagcgc
     1741 atagacgggc agcggctgcc cggacgccag cgcggcaaat tcagaccacc ccagcggcac
     1801 cgaatcggca tgactgcgcg taggcagaac cggcccgagc agcgcgtagt cgagaccgag
     1861 cgataccgcc tgcgcgagtt cggccggtga gtggcaagac gcgccgacca acgcgaaggg
     1921 tgggcgggcg gcgcggcgca tcagctcggt gctggccaga tgtacgccgt cggcggcgat
     1981 gtccgcggcc agctgcacgt cggaattgac cagaacgcgc gcgccagccg gccgcgccag
     2041 atcaatcgcg cggcgcgcaa acagcgcccg ttgtggctcg ggcagcgtct tgtcgcggat
     2101 ctgcagcagg cgcaggccgc cggcgagcgc gcgctgaaga cgctcgagtt cttcttcgac
     2161 gccgtgctcc tcggcgcagc tgatcgcgta gcgcgtcggc aggtcgagcg cggccatgat
     2221 ctgcgtgttg gccggcagca ccggcgcgac actgggtgct tgatggacgc ggcaccaggc
     2281 gaagccgctg tgctcgatcg gcgcgatcgt gccgtaccag cggcggatgc ggaagaagtg
     2341 caggcgcacg cgtgcgtgcg ggtagtcgaa ctcgcgaacc aaccagggcg tcgcgtcggc
     2401 aacggtgatc ccgagttcct cggtcagctc gcgcaccaac gcatcgtgtg gcgattcgcc
     2461 ggcctcgacc ttgccgccgg ggaactccca gtaaccggcg tagaccttgc cgggcggacg
     2521 ctgggcgagc aggtattcct cgcgcagatc attgtcggcg ccgcgcagca atactgcggc
     2581 ggcgacttcg gtgaccggac gcggcgtgcc gctcaccgtc cggcccgctt tggccggcgg
     2641 ggcagccgct cttggcgccc cgcccagtcg cgggcgaact gccagccgac tcggccgttg
     2701 cgggcgccgc ggccgagtgc ccagttgagc gcctcttgcc gtgccacttc tacctgtttc
     2761 gcgttggcgc cgtagtgctg cagccagtag gcgacgatgt cgaggtaggc gtcctgatcg
     2821 aaggggtaga aggagagcca gaggccgaag cgctcggaga gcgaggtttt ttcctcggtg
     2881 ctctcgcccg ggtggatctc gccgccatgg cgttgtgtct cgaggttctc ggcgaagaac
     2941 tcgggcatca ggtggcggcg gttcgaggtt gcgtagatca gcaggttgtc gggcggtgcg
     3001 gcgagcgagc cgtcgagcgc gaccttgagt tccctgaagc tcgcgtcgcc ggcctcgaag
     3061 gataggtcgt cgcagaagac gatgaagcgc tcgggccggc cgtcgaccag cgcgatcagg
     3121 tccgggagat cggcgaggtc gtggcgatcg acctcgatca tgcgcaggcc gcggtcggcg
     3181 aaggcatgca gcagcgcctt caccaacgag gacttgcccg agccgcgggc gccagtcagc
     3241 agcgcgttgt tggccgggcg gcaggcgacg aactgccggg tgttggcgac caggcgatcc
     3301 ttctgttcgt cgatatcgcg caggtcctcg aggcggatcg ggtgcggatg ggcgatcgcc
     3361 tgcagcacgc ctcgggaacc gacgcgccgc cagcggaagg cgattgcgca ctcccagtcg
     3421 accgcttctg gtggcggcgg cagcaccgct tcgaggcgct cggcgagggc ttcggcacgt
     3481 gccagcaggc gggcgagcga cagggagtcg ggcgcagcgt gggggggctt cttggctcgg
     3541 tcggcgggca tcgatcgttg gtctttggcg gaggccgcat tgtcgccgaa ctcggcggcg
     3601 acgcgaaacg cgcccctctg ccgcgcgccg tcgcctacgt cttggcggct cgatgctctg
     3661 gtgcgccccg gtatactcgc gccgcatgac ccacccaatg actcgccgct tgtcctcccg
     3721 cccagccgcc accgttttct tttcccttcg ccgtttcgcg cccctgctgg cactcgcact
     3781 cggtgcctgt gcgctggtcg gtccgccgcc aaccgggcac tgtcccggct gcgtcgcgcc
     3841 gccgcccgaa ccgcctctgc cagcgccggt catttcacca gcgagctggc aggaccttcc
     3901 gggctggaca ggcgacacgc tcgacggcgc ctgggcggct tttcgccaga gctgtgagtc
     3961 gggacggtgg aagaacgcga tgagcaacgg cagcgccgcc gccggcgcga aggcgctgcc
     4021 gaccgcggtc acggctcccg cgtcgggcgg cgccgcgccg ccatcggtcg tggcgcagac
     4081 ttcggcggtc ggtggcagcg catcacccaa tggcaatgtc gcggccggca atggcacggc
     4141 gccgccggcc tacgactggg gcgcagcctg cacggccgcg cgccagcaag gcgacgtcaa
     4201 accctccgcc gccgtccttc gcgacttctt cgaacatcat ttcgtgccct ggtcgctgac
     4261 caatcccgac ggcacgcaag agggcacggt caccggctac tacgaaccac tgctgcgcgg
     4321 cagccgcaag cgcgagggag cgttcactgt gccgatccat gcggtgcccg acgacctgct
     4381 gaccatcgaa atgggcgacc tgtttcccga gctcaagggt aagcgcgtgc gtggccgcgt
     4441 cgagggcaag cgcgttgtgc cctactacga ccgcggcggc atccgcgcgc gcgccgccga
     4501 actgcgcgag aaggtactgg tctggggcga cgatgcggtc gaggtcttct tcctgcagat
     4561 acaaggttcc gggcgcgtcc gcctgcccga tggctcgctg atgcgcatcg gctacgcaga
     4621 ccagaacggc catccctaca actcgatcgg ccggctcctc gtcgagcgcg gcgacctgct
     4681 gctcgagcag gcttcgatgc agggaatcca ggcctgggcg cgcgccaatc ccggcaagct
     4741 tgaggcgctg ctcgatgcca acccgagcta cgtctttttc cgcgaattgc cagccggcga
     4801 tcaggggccg atcggcgcgc tcggcgtgcc gctgacggcg gggcgttcga tcgccgttga
     4861 cccggccttc gtgcctctcg gcgcgccggt attcctggcc accaccgagc ccaacagcgc
     4921 cgtgccgctc aaccggctaa tgttcgcgca ggacaccggc ggcgcgatca agggcgtggt
     4981 ccgtgccgac ttcttctggg gtttcggccc cgaggccggc gcgctcgccg gccgcatgcg
     5041 gcagatcggc cgcatgtggg tactgctgcc gcggactgcg gttgcctctg acctaccgcc
     5101 gatcgcgcgg cggtaagcgc ccgccaacac gcactatttc ggcgtccgcg caccaaacag
     5161 gtgtgctgac gcactacctt ggtgcgttat ccgccgttga tccattcgta agtcattgta
     5221 ttttcatgat ggtcggctgg aacgaccctt gctcaacttg gcaccgaatt gaactcagga
     5281 ggtcttatgg acgcactcaa atcgggcagt gacacgctct ttgtcctgct tggcgccatc
     5341 atggtgctgg caatgcacgc gggctttgcc tttctcgagg tgggcacggt tcgccgcaag
     5401 aaccaggtca atgcactggt caagatcatc gccgacttcg cgatgtccac gatcgcttac
     5461 ttcttcgttg gctacagcgt tgcctacggc gtcagcttct tttcaggcgc cgaaaccttg
     5521 gccgcgaaaa acggctacga gctgaccaag ttctttttcc tccttacctt cgccgcggcg
     5581 attccggcaa tcatctccgg cggcatcgcc gagcgggcgc gtttcaatcc gcagcttgcc
     5641 gccaccttcg tcctcgtcgc gctcgtttac cccttcttcg aaggcatcgt ctggggcggc
     5701 gcctacggcg ttcaggaatg gcttgccgcc accttcggtg ccagcttcca cgactttgcc
     5761 ggctcggtcg tcgtgcacgc gatgggcggc tggctggcat tgccggccgt gctgctgctc
     5821 ggcgcccgcc gcggccgcta cgggaaggac ggcacagtct cggcgcaccc gccctcgtcg
     5881 attcccttcc tcgcgctcgg cgcctggatc ctcacggtcg gctggttcgg ctttaatgtc
     5941 atgagcgcgc aggcgatcga aggcatatcc ggcctcgttg ccgtcaactc gctgatggcc
     6001 atggtcggtg gcacgctcgc cgcgctcgcc gccggcaaaa acgacccagg cttcgtgcat
     6061 aacggcccgc tagccggcct ggtcgccgtc tgcgccggtt ccgacctgat gcacccgctc
     6121 ggcgccttga tcgtcggcgg cgtcgccggc gggcttttcg tcctcatgtt cacgctcacg
     6181 cagaaccgct ggaagatcga cgacgtgctc ggcgtctggc cgctgcacgg gctgtgcggc
     6241 gcttggggcg gcatcgccgc cggcatcttc ggtctgcagg cgctgggcgg aatgggcggc
     6301 gtcagcttca tggcgcagct gatcggcacg ctgatgggcg tcggtgtcgc actggtcggc
     6361 ggcttcgtcg tctacggtac gattaagcag atcatgggcc tccgcctcga cgccgaggat
     6421 gaattcaacg gggccgatct cgccatccac cgcatttcat cgacggccga gcgcgagaca
     6481 ctgtggtgat tctgaaaaga gaaaataaag tctgtcggag cggaataaag tctgtcgaag
     6541 tactcgctct cacgaagaag ccggcctaga gccggttttt tcgttttggt gagatgacaa
     6601 acgggaatcg gcggttttga cgcttcgatg atccgctgaa atcacctgaa tgctctgacg
     6661 ttcaccctgt ttccgcgggt gttgtaagcg atcgagcggc gcctcctcgg ccattccggc
     6721 ctgtcacgcg tagcagttcg attgaccgct gtcagccggg ttgctgaccg tcggactcag
     6781 cgactgcttt cgggtggtct gcgcgagggt cgtaggaccg gacccggatc taggagacgc
     6841 tcggctgtga tggtccaacc gtccgcttct ggccgggcgg agaagttggc gttccgatgt
     6901 cgagtggctg gagccctctg gacgcttgcg ctcgtttacg aaaacggcga cgcgaggcga
     6961 atgaccgccg acaacaatgg gatcaactct acgtcagttt tcggaaaaat cgcgggcggc
     7021 agatcaccgc cgacttgagg cccatgcgct cacctcaacc caccttgtcg cacggaggtc
     7081 gccgacgtca gggacataga gggggcgcgt ggtcacttga gaggcagccg tgaatgggcc
     7141 ctattccgcg ggcgccgtca cccaaagcgt aagaacgccg cctgacccca gcattagcgg
     7201 cggtgcgaag cgccccgggt gcggtgttgg gcggctttct gcttgaatcg ccagtcaggc
     7261 cgacgctatt tgaggtgggt gatgaccgtc ccagtggaca catgcggcgc actgacgc
//
LOCUS       DATDAA010000068         5591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_494081,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000068 DATDAA010000000
VERSION     DATDAA010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494081"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VIS73_02180"
     CDS             <1..796
                     /locus_tag="VIS73_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761995.1"
                     /translation="VGKVRDISLNDKDASETLIRIRVLKSVPITKKTRAQLAYQGVTG
                     IAHILLIDDGKSQVPLVAANGSLPRIPMEASFFDRLGDAGGNVIQQSGELLVRAQSLL
                     DEENQLAIRAILGNLQQLSGQLVELGERTVPLMTRVDAVLLETEALLAEENRLQLARS
                     LDSFAVASEAATGVFERWQRLTDRYDALAVQLDEAARRATGPQGVGGAAARVQQLASE
                     LEQTSRRLNRVLGDIEDAPNSLIVGAPVTAPGPGEAGFRAPPVREK"
     gene            801..1397
                     /locus_tag="VIS73_02185"
     CDS             801..1397
                     /locus_tag="VIS73_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761996.1"
                     /translation="MNAILSSGKRVLIGAAVLLLAACADLPKAPPAKAVYDFGLPAER
                     LPGADLSAVVLVVGSPPWMDERDMLYRLSWDDPQLRRSYAASRWAATPSELLALRWRQ
                     RLGLVQPGEGRGCQLRLRIDEFSQVFAAADSSEAILDAAVILFDARGKVLARHQLAAR
                     RPAGADGRSGAAALAQVADSAASEIRRWLAEPAAAGCL"
     gene            1579..3564
                     /locus_tag="VIS73_02190"
     CDS             1579..3564
                     /locus_tag="VIS73_02190"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEY5761997.1"
                     /translation="MREQTRPLWQPPADAASTTRLGAFMRHAGAAANTRFDNYESLWR
                     WSVGERAAFWQEVWTFFEVLGSRGEQVVKTAHSIRETRWFPDARLNYAENLLRHCQSG
                     DTRDALVFHGENRVVARLSRGELHDAVSRCQQALLAVGVGEGDRVAGFLPNMPEAIVA
                     MLATASIGAVWTSASPDFGVQGVLDRFGQTEPRVLFCVDAYWYNGKVVDCREKNREIA
                     GRLPSLAAVVEVAYAELPEAAAPAASTVEVRSWGDLLRGFVTAPITFHRVPFDHPLFI
                     MYSSGTTGVPKCIVHGHGGTLLQHLKEHQLHGDLRPGDRIFYFTTCGWMMWNWLASGL
                     ASEATLLLYDGSPFAKGRTSGNAVFDYLAEEGATHFGTSAKFLDAAAKAGLAPAKTHR
                     LDALRVVFSTGSPLAPEGFDYVYDAIKPDVLLASISGGTDILSCFVLGCPLLPVWRGE
                     IQCRGLGMAVEVFDEDGRPVRGEKGELVCTKSFPSMPVGFWNDPEGEKYRNAYFEQFP
                     GVWHHGDFVEMTEHGGMIIYGRSDATLNPGGVRIGTAEIYRQVEQIDAVVESLVIGQD
                     FDKDVRVILFVKLREGQTLDEALVERIRRAIRDNTTPRHVPARVLQVADIPRTKSGKI
                     VELAVRNVVHGRPVKNIEALANPEALADFRDRPELSH"
     gene            3699..4385
                     /locus_tag="VIS73_02195"
                     /pseudo
     CDS             3699..4385
                     /locus_tag="VIS73_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
     gene            4845..5171
                     /locus_tag="VIS73_02200"
                     /pseudo
     CDS             4845..5171
                     /locus_tag="VIS73_02200"
                     /inference="COORDINATES: protein motif:HMM:NF017054.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
     gene            5433..5591
                     /locus_tag="VIS73_02205"
                     /pseudo
     CDS             5433..5591
                     /locus_tag="VIS73_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933041.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
BASE COUNT          957 a         1778 c         1848 g         1008 t
ORIGIN      
        1 ggtcggcaag gtccgcgaca ttagtctcaa cgacaaggat gcgagcgaaa cgctgatccg
       61 catccgcgtg ctgaagagcg tgccgatcac aaagaagacg agggcgcagc tcgcttatca
      121 gggtgtcacg ggcatcgccc acatcctgct gatcgacgac ggcaagtcgc aggtgccgct
      181 ggtcgctgcc aacggcagcc tgccgcgaat cccgatggaa gcctcgttct tcgaccgctt
      241 gggcgatgcc ggtggcaacg tcatccagca gagtggcgag ttgctggtac gggcgcagag
      301 cctgctcgac gaagaaaacc agttggcgat ccgcgcaatt ctcggcaacc tccagcaact
      361 cagcggccag ctggtcgagc tcggggagcg taccgtaccg ttgatgacgc gcgtggacgc
      421 cgtgctgctc gaaaccgagg cgctgcttgc cgaggagaat cgcctgcagc tggcgcgcag
      481 cctggactcc tttgcagtgg cgtcggaagc ggcgaccggc gtgttcgagc gctggcagcg
      541 ccttaccgat cgatacgacg cgctcgccgt acagctggac gaggcggcgc gccgtgccac
      601 agggccgcag ggcgttggcg gcgccgccgc ccgcgtgcag cagctggcca gcgaactcga
      661 acagacgagc cgtcgcctca atcgtgtgct tggcgacatc gaggatgcgc cgaacagcct
      721 gattgtcggc gcgcccgtga ccgctccggg gcccggtgag gccggcttcc gcgcaccgcc
      781 tgtgagggag aagtgaagcg atgaatgcca ttctgtcatc gggaaagcgg gtcctgatcg
      841 gtgccgctgt gctgctgctc gccgcctgtg ccgacctgcc gaaggcgccg ccggccaagg
      901 ccgtctacga cttcggcctg cccgccgagc gcttgcccgg tgccgatctg agcgccgtcg
      961 tgctggtcgt cggttcgccg ccgtggatgg atgagcgcga catgctctac cggctgagct
     1021 gggacgatcc gcaactgcgg cgcagctacg cggcgagtcg ctgggcggcg acgccgagcg
     1081 agttgctggc gctgcgctgg cggcagcgcc tcggcctcgt gcaaccgggc gagggcaggg
     1141 gctgccagct gcgcctgcgc atcgacgaat tcagtcaggt cttcgctgcc gcagattcca
     1201 gcgaagcgat cctcgacgcc gcggtgatcc ttttcgatgc gcgcggaaag gtgctggcgc
     1261 gacatcagct cgccgcccgc cggccggccg gtgccgatgg ccgcagcggt gccgcggcgc
     1321 ttgcgcaggt tgccgattcc gcggcctccg aaatccgccg ctggcttgcc gagcccgccg
     1381 cggccggctg cctgtagggg cgccaccggc cgaatcagga tctggctggc gcagcggcga
     1441 taaagccgcg ccgattccgc tggtccggat cagggtcttc catacggcac cgtattggaa
     1501 aaggcctcgt cgtggcatag acttcgcgct tgcaggccac gcctgcggcg aggaccatgt
     1561 ctacccagag gaggatcgat gcgcgagcaa acacggccct tgtggcagcc gccggcggat
     1621 gccgcatcca ctacacggct cggtgccttc atgcgccacg ctggcgctgc agctaacacg
     1681 cgcttcgaca actacgagag cctgtggcgc tggtcggtcg gcgagcgggc ggcgttctgg
     1741 caggaagtct ggaccttctt cgaagtgctc ggcagccgcg gtgagcaggt ggtcaagacc
     1801 gcccattcaa tccgcgagac ccgctggttt ccagacgccc ggctcaatta cgcggagaac
     1861 ctgctgcgcc attgccaatc gggcgatacg cgcgacgcgc tggtattcca tggcgagaac
     1921 cgcgtggtcg cccggctgtc gcgcggcgaa ctccacgatg cggtgagccg ctgccagcag
     1981 gcattgctgg cggtcggcgt cggcgagggc gatcgcgttg ccggttttct accgaacatg
     2041 cccgaggcca tcgtcgccat gctggcgacg gcgtcgatcg gtgccgtctg gacatcggcc
     2101 tctcccgact tcggcgtcca gggcgtgctt gatcgtttcg gccagaccga gccccgggtg
     2161 cttttttgcg ttgatgccta ctggtacaac ggcaaggtcg ttgactgccg agaaaagaac
     2221 cgcgagatcg ccggccggtt gccctcgctc gctgcggtcg tcgaggtcgc ctacgccgag
     2281 ttgccggaag ccgcggcgcc ggccgcttcc acagtcgagg ttcgctcatg gggcgatctt
     2341 ctgcggggtt ttgttacggc gccgatcacc ttccatcgcg ttcccttcga ccatccgctg
     2401 ttcatcatgt actcgtcagg aaccacaggc gtgcccaagt gcatagtcca cgggcacggc
     2461 ggaacgctgc tgcaacacct gaaggagcac cagttgcacg gcgacctgcg gcccggcgac
     2521 cgcatcttct acttcaccac ctgcggctgg atgatgtgga actggctggc ttcgggcctg
     2581 gccagcgagg cgacgctgct gctctacgac ggcagcccct ttgccaaggg caggacgagc
     2641 ggcaacgcgg tcttcgacta tctggccgag gagggcgcca cgcatttcgg tacctcggcg
     2701 aagtttctcg acgccgccgc caaggccggt ctcgcgccgg cgaaaaccca tcggctcgat
     2761 gccctgcgcg tggttttctc caccggcagc ccgttggcac ccgagggctt cgattacgtt
     2821 tacgacgcga tcaagcctga tgtgctgctc gcctcgattt ccgggggaac cgacatcctt
     2881 tcctgcttcg tgcttggctg tccgctgctg ccggtctggc gcggcgaaat tcagtgtcgc
     2941 gggctgggca tggcggtcga ggtgttcgac gaggatggcc gtccggtccg cggcgagaag
     3001 ggcgaactgg tctgtacaaa gagctttccg tcgatgccgg tcggtttctg gaacgatccc
     3061 gaaggcgaga aataccgcaa tgcctacttc gagcagttcc caggtgtctg gcaccacggc
     3121 gacttcgtcg agatgaccga acacggcggc atgatcatct acggccgctc ggatgcaacg
     3181 cttaaccccg gcggcgtacg catcggcacc gcggagatct accggcaggt cgagcagatc
     3241 gacgccgtag tcgagtcgct ggtgatcggt caggatttcg acaaggacgt gcgcgtcatc
     3301 ctcttcgtca agctgcgcga gggccagacg ctcgacgagg cgctggtcga gcgcatccgc
     3361 cgcgcgatcc gcgacaatac gacgccgcgg cacgtgccgg cgcgagttct gcaggtggcc
     3421 gacatcccgc gcaccaagag cggcaaaatc gtcgaactgg cggtgcgcaa tgtcgtgcac
     3481 gggcgtccgg tgaagaacat tgaggccttg gccaaccctg aggcattggc cgacttccgc
     3541 gatcgacccg aactaagtca ctgatttcgg gcgctgccga tccgggctaa tgattgatgg
     3601 cccgacccgg cggcggagcg ggtaaacttc gtcgctcacc ggccgcgcat tccgcccgaa
     3661 ctgccgcatc gaatcagcca cggaagccac cccagtccat gtccaaggtc tttaccggta
     3721 ccgaccccga gatcgccgct ttgcggattc cgccgcactc ggtcgaggcc gagcagtcgg
     3781 tgatcggcgg cctgctgctc gacaatgccg cctgggaaaa catctcgggc atgatcgacg
     3841 agagcgattt ctatcgcgac gagcatcgcc gcatctttcg ccagatagtc cgcctgctcg
     3901 ataaggccaa gccggccgac gtcgtcaccg tatccgaagc gctcgatgtc tcgggcgacg
     3961 gcagcaacac cggcgggctc gcctacctcg gcgagctggc ggcgaatact ccatcggccg
     4021 ccaacatccg ccgctacgca gagatcgttc gcgagcgggc gatcctgcgc aagctggtta
     4081 ccgccaccga cgagatcgcc cagtcggcgt tcagcccgat gggacgcgat gcgaaaaccc
     4141 tgctcgacga ggccgaagcg aaggtcatgt cgatcgccga ggccggcgcg cgcaaccagg
     4201 gcggattcca gcacatcaac ccgctgctta acgaagtcgt cgagcgcatc caggaactcc
     4261 acgaccgcga taatccatcg gacatcaccg gcgtgccgac gggtttcatc gatctcgatt
     4321 cgatgacctc ggggttgcag ggcggcgacc tgatcatcgt tgccggaagg ccctcgatgg
     4381 gcaaggcgca gccactcgac gcgcgggttc gcgttccggg cggctggaag tcgatgggcg
     4441 aactggaggt cggtgatgcg ttgtcgtcga tcgacggggc accatcgatt gttacgggcg
     4501 tattccctca aggtgaacgg caggtctatc gcgtccgttt ttccgatggt cggagcaccg
     4561 agtgttgcgc agagcacctg tggcgcgtcc attaccggga atgggttcag ccgcgcgtga
     4621 tcgacaccgc aaccctgatc tcgatgctcg ggaaggtccg gtatcgaaat cgcttgtgga
     4681 tcgatgttgc cgatccgcga tttggagatg ccgagtcctt gccccttgat ccgtggctgt
     4741 tgggtgccct gcttggagac ggatcactgg caggttcggc gctgcggttc tccatggcca
     4801 gcgacgaaat gcttggcaag ctcaaacagc gtgcaggcga gggaatggct gtagttcacg
     4861 cgggaggtta cgactaccga atcgtgccgg aggattgcgg gcaccgcgcg ggattcacgg
     4921 gtagccaaac taacccgttg accaaggcgc tgcgcgagct tggtttatgg gatgtggata
     4981 gcagctcgaa attcattccg gagtgctacc tcacggctga cgccgagtcg aggcgactgc
     5041 tgctcgcggg attgttggat accgacggtt gggtagagcg ttggggcagc gtgcgaattt
     5101 caacatcgag ccgacgcttt gccgacggta ttgccgagct ggcgcgatca ttgggtgcct
     5161 ggtgctcgat tagggaaaaa ccgcccgttt gttgctcgca tggcgagaag aaacccggga
     5221 agcctgcgtt cgtttgcaac ataagccatc cccggccgaa gtccctgttc ttgctctcgg
     5281 acaagcaaat gcgggcaccg gagcggtggg ctcggcagaa gcggctgacg tttgctgaca
     5341 tcacgccgtc acgtgtcaca acgtgccaat gcatttcagt cagtcaccca acgcgttgct
     5401 acgtgactga ccagcacatc gttacgcaca acaccgcctt cgcgctgaac atcgccgagc
     5461 acgtggcagt cgatgtcggc ttgccggtcg ccgtattctc gatggaaatg ggaggctcgc
     5521 agctggcgat gcgtatgctc tcgtcgatcg gtcgcctcga ttcccaccgc gtgcgcaccg
     5581 gccggctgac c
//
LOCUS       DATDAA010000069         3641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_354467,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000069 DATDAA010000000
VERSION     DATDAA010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354467"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VIS73_02210"
     CDS             <1..1176
                     /locus_tag="VIS73_02210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEY5761998.1"
                     /translation="ASVMATGTLSQPSLGANSVFRATGGQAVDTSNVTPATTDFSQAG
                     PGTIDTDEVDPVAEAEVYMAYGRDAQAEEILLEALQKDPKRTAIHGKLLEIYSNRKSL
                     KQFETLATELYAQTGGEGADWEAAAALGAQLDPSNSLYTGAAAPAPMAEKAERTTRTT
                     STVTMPGELAQMAETAGAVEASGGVDFDLGGNDAEPAADKQIFDFDLGLGGDGKDQKP
                     ASNTAQQSTIVLPSEATDSHGDGKSADEGLNFDFNVGAGADTPVDFELMSSQIAPEPI
                     KPEAPVNLSGISLDLDKEPAAADEQAEAEGGDFDKTMLFTPGSLPDAEPTAGASPTVE
                     SDGFDSNVQAVATKLDLAKAYQEMGDAEGARELLDEVVAEGNQEQRRIAKEIIERLA"
     gene            1263..1874
                     /locus_tag="VIS73_02215"
     CDS             1263..1874
                     /locus_tag="VIS73_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5761999.1"
                     /translation="MTALQLHPVTRLLLWCNWVVAAFILPLAVIVASLPLILLGIDTQ
                     ARRRWFGLAWRSRWLMAAILLAISLSVPGQYLIPGYPGTHEGVAAALEQIGRLLVTLA
                     AVAWLLAAQRSELVAAIYGAGLALGGGRGARDLERFALRVLLVFELVEDRRIHWAELL
                     AADSMDIGGGALMIELRSFSMPDRLINLLGVAVLLVVGVWGLR"
     gene            1871..2683
                     /gene="truA"
                     /locus_tag="VIS73_02220"
     CDS             1871..2683
                     /gene="truA"
                     /locus_tag="VIS73_02220"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY5762000.1"
                     /translation="MTERGLLRIAIGLEYAGESYCGWQRQPNAPSIQACVETAISRIA
                     GVDVAITAAGRTDSGVHASAQVVHADVPGDRPMTAWVRGVNSHLPNDIAVNWAVQVSP
                     DFHARFRATGREYRYFLLNRPTRPAVESHRVGWHHRPLDLEAMQRAAACLIGQHDFSS
                     FRAAECQAKTPVRELRRLVIARDAVPGGYRFAITLEADAFLHHMVRNIVGALVAVGNG
                     GRRPEWVAEVLAEKDRRLAAPTFAPDGLYLSGVAYDAHWGLPAGGPSVLVSP"
     gene            2680..3330
                     /locus_tag="VIS73_02225"
     CDS             2680..3330
                     /locus_tag="VIS73_02225"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY5762001.1"
                     /translation="MTTRIKICGITRVEDGLACAALGADAIGLVFYPASPRFVEAATA
                     RDIARSLPPFLTRVGLFVNPRADEVRQVLEEVELDVLQFHGDESPAFCGQFRRPYLKA
                     ARVRPGFDLVEYARRFATDDALRAQAILCDADVKGFGGGGMVFDWGLLPDSLPLPLIL
                     SGGLSPENVAPAIRRVRPLAVDVSSGVERDKGIKDRAKIERFIHEVRAVDEELRLS"
     gene            3308..>3641
                     /locus_tag="VIS73_02230"
     CDS             3308..>3641
                     /locus_tag="VIS73_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY5762002.1"
                     /translation="MKNYDFPDASGHFGPYGGVFVSETLMAALAQLREAYRYAKDDPE
                     FKAEFAYELKHYVGRPSPVYHARRWSEILGGAQIYLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRV"
BASE COUNT          632 a         1128 c         1207 g          674 t
ORIGIN      
        1 gcgtcagtca tggcgaccgg aacgttgagt cagccaagcc tcggggcaaa ctcggtattt
       61 cgggcgaccg gcggccaggc cgtcgacacg tctaacgtaa cgccggcgac cactgacttc
      121 agccaggcgg gaccggggac catcgacacc gatgaagtcg atccggttgc cgaggcagag
      181 gtgtacatgg cctacgggcg tgacgcccag gccgaggaaa tcctgcttga agcgcttcag
      241 aaggatccca agcgcacggc gatccacggc aagctgctgg agatctactc caaccgcaag
      301 agcctcaagc agttcgaaac gctggcgacc gagttgtacg cgcaaaccgg cggcgaaggt
      361 gcggattggg aagcggcagc agcgctcggc gcccagcttg acccgagcaa ctcgctctat
      421 accggcgcgg cggcgccagc gccgatggcc gagaaggccg agcgcacgac ccgaaccacc
      481 tcgacggtga cgatgccggg cgagctggcg cagatggccg aaacggcagg cgccgttgaa
      541 gcatccggcg gtgtcgattt cgatctgggc gggaacgatg ccgagcccgc cgccgacaag
      601 cagatctttg atttcgacct cggtctaggc ggcgacggca aggatcagaa accggcctcc
      661 aacacggcgc agcaatcgac gatagtcttg ccgagcgaag cgaccgatag ccacggtgat
      721 ggcaagagcg ccgacgaggg gctcaatttc gacttcaacg ttggcgccgg tgccgatacg
      781 cccgtcgatt tcgagctgat gtcatcgcag atcgcgccgg agccgatcaa gccggaagcg
      841 cccgtcaatc tgtcgggcat cagccttgat ctggacaagg agcctgccgc cgctgatgag
      901 caggccgagg ccgagggcgg ggacttcgac aagaccatgc tattcacgcc aggcagcctg
      961 cccgatgccg agccgacggc cggcgccagc ccgacggtcg aatcagatgg tttcgacagc
     1021 aacgttcagg cggtcgcgac caagctcgac ctcgccaagg cataccagga gatgggcgac
     1081 gccgagggtg cgcgggagct gctcgacgaa gtcgtcgccg agggcaatca ggagcagcgc
     1141 aggatcgcca aggaaatcat cgaacggctc gcctagcacc gcgaccgttg ccgtacccga
     1201 agcgccgcgg tcggtgatcg cggcgttttc ctttccgatt ccggcggagc acggaagatc
     1261 gtatgacagc tttgcagctt cacccggtga cgcgactgct actctggtgc aactgggtcg
     1321 tcgcggcttt catcctgccg ctcgcggtca ttgtcgcctc gctcccgttg atcctgcttg
     1381 gaatcgacac gcaggcccgc cggcgctggt tcggacttgc gtggcgctcg cgctggctga
     1441 tggcggcgat tctgctggcg atcagtctgt cggtgccggg gcagtatctg atacccggct
     1501 acccgggtac gcacgaaggc gtcgccgcag cgcttgagca gatcggccgc ctgctggtca
     1561 cgctggcggc ggtagcctgg ctgctggccg cgcagcggtc cgaactcgtg gcggcgatct
     1621 atggtgccgg ccttgcgctc ggtggcgggc gcggcgcgcg cgatcttgag cgcttcgcgc
     1681 tgcgcgttct gctcgtattc gagctggtcg aggaccggcg aattcactgg gccgagctgc
     1741 tcgcagccga ttcgatggac attggtggcg gtgcgctgat gattgagctg cgctcatttt
     1801 cgatgccgga tcgcctcatc aatttgctgg gcgttgccgt cctgcttgtc gttggcgtat
     1861 gggggttgcg gtgaccgagc gtggccttct tcgtattgcc atcggcctcg aatatgccgg
     1921 ggaatcctat tgcggctggc agcgccagcc aaatgcgcca tcgattcagg catgcgtcga
     1981 aacggcaata agccggatcg ccggtgtcga tgttgccatc accgccgccg ggcggaccga
     2041 cagcggtgtg catgcaagcg ctcaggtcgt ccatgccgac gttcccggtg atcgcccgat
     2101 gacggcttgg gtccgcggcg tcaacagcca cttgccgaat gatatcgcgg tcaactgggc
     2161 ggttcaggtc agtccggatt ttcatgcgcg ttttcgtgcg accggccgcg agtaccgcta
     2221 cttcctgctc aaccgaccca cccggccggc cgtggagagc caccgcgtcg gctggcatca
     2281 tcgaccacta gaccttgagg cgatgcagcg cgccgccgcc tgcctgatcg ggcagcatga
     2341 tttcagttcg ttccgcgccg ccgaatgcca ggcaaagacg ccggtgcgcg agctgcgccg
     2401 cctggtgatt gcgcgcgatg ccgtaccagg tggttaccgt ttcgccatca cccttgaggc
     2461 cgacgccttt ctccaccata tggtgcgcaa catcgttggc gcgctggttg ccgtcggcaa
     2521 tggcggccgc cgccccgagt gggtcgccga ggtgcttgcg gagaaagacc gccggcttgc
     2581 ggcgccgacc tttgcgccag acggcctata cctcagcggc gtcgcctatg atgcgcactg
     2641 gggtttgccc gcaggggggc catcggtgct ggtttcgcca tgacgactcg aatcaagatc
     2701 tgcggcatca cccgtgtgga ggatggtctt gcctgtgcag cgctgggggc cgacgcaatt
     2761 ggcctggtct tctatccggc tagtcctcgt ttcgttgaag cggccacggc gcgtgatatc
     2821 gctcgatcgt tgccaccgtt tctcacccgc gtcgggctgt tcgtcaatcc tcgggccgac
     2881 gaggtaaggc aggtgctcga agaggtcgag cttgacgtgt tgcagtttca cggcgacgag
     2941 tcgcccgcgt tctgcgggca gttccggcgg ccctacctga aagccgcccg cgtcaggccg
     3001 gggttcgatt tggtagaata cgcgcgccgt ttcgccaccg acgacgcgct gcgtgcgcag
     3061 gcaatactct gcgatgcgga cgtcaagggt tttggcggcg gcggcatggt attcgactgg
     3121 ggtctgctac ccgactcact tccattgccc ctgattcttt ccggcgggct ctccccggag
     3181 aacgtcgcgc cggcaatccg acgcgtgcgc ccgctggcgg tcgatgtcag ttccggtgtc
     3241 gagcgggaca agggaatcaa ggacagggcg aagattgaac gattcattca cgaggttcgc
     3301 gcagtcgatg aagaactacg actttcctga cgcatcaggg catttcgggc cgtacggtgg
     3361 ggtcttcgtc tcggagacgc tgatggccgc acttgcgcag ctgcgtgagg cctatcgcta
     3421 cgccaaggat gatccggaat tcaaggccga gtttgcctac gaactcaagc actacgtcgg
     3481 tcggccgtcg ccggtctatc acgcgcgacg ctggtccgaa attcttggcg gtgcacagat
     3541 ctacctcaag cgtgaggatc tgaaccatac cggtgcgcac aaggtgaaca acaccattgg
     3601 ccaggcacta ttggccaagc gtatgggtaa aaagcgtgtg a
//
LOCUS       DATDAA010000070         5855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_144474,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000070 DATDAA010000000
VERSION     DATDAA010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144474"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VIS73_02235"
     CDS             <1..462
                     /locus_tag="VIS73_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762003.1"
                     /translation="FSFDAIVKRTPWHSIGFDFFSFYSRFDTPAHLQSPLFGTLDSRA
                     SLTGGGFSASSRAHLPVGRIELHAGAGIGVFFSRLRVSGATFGLPGSSEQRSTDIGWQ
                     LMAGFSHHALPRRSWGLEWRYLNVQADFGTLTNGSVQIGGQSVMLFGRLEG"
     gene            complement(476..1501)
                     /gene="moaA"
                     /locus_tag="VIS73_02240"
     CDS             complement(476..1501)
                     /gene="moaA"
                     /locus_tag="VIS73_02240"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY5762004.1"
                     /translation="MNAPDPIALAGPRSALIDALGRRVSYLRLSVTDRCDFRCTYCMS
                     EKMSFLPHSALMSLEESLQIVETFVALGVSKVRITGGEPLVRRNLVWLLERIAALPGV
                     RELVLTTNGSQLAENAEALRAAGVRRLNISLDTLNPERFRRITRTGRLENVIAGIDAA
                     RAAGFERIRLNALLLRDGNADELADLVQFAVDRGIDVGFIEEMPLGQVAGRQQPLKAD
                     EARRLMAARFPLHPVDESTGGPAEYWRIEGSSSPSRIGFITPHSHNFCASCNRVRVTA
                     QGDLYPCLGNDGMVPLLPVLRNTGGDTVALHRAIVGCLMRKPDGHRFTEQMQAPKVMR
                     HMSVTGG"
     gene            complement(1659..3392)
                     /locus_tag="VIS73_02245"
     CDS             complement(1659..3392)
                     /locus_tag="VIS73_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent methanol/ethanol family
                     dehydrogenase"
                     /protein_id="HEY5762005.1"
                     /translation="MSVLVAGALVAAATGSSYAGVTDADIEKDAMTTGDVLSWGMGTE
                     GQRYSPLTQVNTGTVKNLVPAWAFSFGGEKQRGQESQPLLVNGKLLVTGSYSRAWAID
                     AKTGAELWQYDHRLPDGIMPCCDVINRGGAVYDNLFIFMTLDAQIVALDVDSGKVVWK
                     EKIDDYKAGYSATAAPIIANGLVLTGVSGGEFGVVGRVEARDAKTGKMVWVRPTVEGH
                     MGYTYAKDGGKKENGVSGTTNQTWPGDLWQTGGAATWLGGSYNKETGLAYFGTGNPAP
                     WNSHLRPGDNLYSCSTVAIDVKTGKIVWHFQGTPNDGWDYDGVNEFVTYKDKSGKILG
                     GKADRNGFFYVLDAKTGKFERGFPFVNKITWAKGLDKNGRPIFDPANRPGDPAKSADG
                     KKGEAIFAAPSFLGGKNQMPMAYSPDTGYFYVPANEWGMDIWNEPVSYKKGAAYLGAG
                     FTIKTIDDYIGAMRAIDPATGKIVWEVKNNAPLWGGVLTTKGGLVFWGTPEGFLKAAD
                     AKTGKEVWSFQTGSGVVAPPITYEIDGEQYIAVASGWGGAVPLWGGEVAKKVNLLNQG
                     GSLWVFKLHKG"
     gene            complement(3621..4541)
                     /locus_tag="VIS73_02250"
     CDS             complement(3621..4541)
                     /locus_tag="VIS73_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     1"
                     /protein_id="HEY5762006.1"
                     /translation="MSWLLALVAAANPASAEDLQYRLQPRQVAADTWVIVGATEDFSM
                     QNGGDIVNTAFIDTGDGVVVIDSGSTRRYGLALRAAIATVTPEPIREIWITHHHPDHF
                     LGNQAFADMPIGALAETARGIRAEGGAFLDNIYRLTGDWAKGTEIHAPETMLAAGHRP
                     IGRHRFRLLALSGHTAADLAVFDETTGVLFAGDLAFHDRAPTTPHAAIGDWQVSLDTL
                     AALPFSLLVPGHGEPTSDDKPLRQTRRWLQWLDQTLRQAAADGLDAAEVLAQPMPDDL
                     ARLPLARRELERSIAHLFGGLEARAVGPAP"
     gene            complement(4589..5368)
                     /locus_tag="VIS73_02255"
     CDS             complement(4589..5368)
                     /locus_tag="VIS73_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HEY5762007.1"
                     /translation="MRRTLSMLLAAALLATSAVHAEPAEPADPLDSPSWPDVRRKLIG
                     DDPVVFDTRVVVLAPAVAEDSMNVPVTVSLRDIEDVRRVLVVADLNPILKVIEFEPLD
                     AVPRISFRIKLQQGSPVRAMAQTGDGVWHVGGTWVDAAGGGCTAPSVGSSQANWSDTL
                     GEVDARWWQREGRGRLKFKVMHPMDTGLAPGIPAFYIERLALRDASGRDWVRLRTFEP
                     LSENPLFSLEFDRPTPPLQLIGVDNNGNRIAAEVSNVGPGQ"
BASE COUNT          948 a         1853 c         1997 g         1057 t
ORIGIN      
        1 ttttcgtttg acgcgatcgt caaacggacg ccatggcatt cgattggttt cgatttcttt
       61 agcttctatt cccggttcga cacacccgcg catctccaat cgccgctttt cggcacgctg
      121 gacagccgcg ccagcctgac aggcggtggg ttttccgcca gttcgcgcgc gcacctgccg
      181 gtcggccgga ttgagcttca tgccggtgcg ggaatcggcg tgtttttcag caggttgcgt
      241 gtcagcggcg cgacctttgg gttgccggga agcagcgagc agcgaagcac ggatatcggg
      301 tggcaactga tggccggttt ttcgcaccat gcgctgccgc ggcggagttg gggtctggag
      361 tggcgctacc tcaacgtgca ggcggatttc ggaaccttga ccaatggttc agtacagatc
      421 ggtgggcaaa gcgtcatgct gttcgggcgt ctggaaggct gaacgctcgg cgggttcagc
      481 cgcccgtcac cgacatgtgc cgcattacct tcggtgcctg catctgctcc gtgaagcgat
      541 ggccgtcggg cttgcgcatc aggcagccga cgatggcccg gtgcagtgcc acggtgtcgc
      601 caccggtatt gcgcagcacc ggcagcagcg gcaccatgcc gtcgttgccg agacagggat
      661 agaggtcgcc ctgggcggtg acgcgaacgc ggttgcagct tgcgcagaag ttgtgcgaat
      721 gcggcgtgat gaagccgatc cgcgacggcg aagacgagcc ttcgatgcgc cagtactcgg
      781 ccggaccgcc ggtcgattcg tcgactggat gaagcggaaa tcgcgcggcc atcagcctgc
      841 gcgcctcgtc agccttgagc ggctgctggc gcccggcaac ctggcccagc ggcatttcct
      901 cgatgaagcc gacgtcgatt ccgcgatcga cggcgaactg cacgagatcg gctagttcat
      961 cggcattgcc gtcgcgcagc agcagcgcgt tgaggcggat gcgctcgaat cccgcggcgc
     1021 gtgccgcatc gatgccggcg atgacattct ccagccgacc ggtccgggtg atgcggcgga
     1081 agcgttccgg gttcagcgta tcgaggctga tgttgaggcg acggacgccg gcagcacgca
     1141 gcgcttcggc gttttcggca agctgcgagc cattggtggt gagcaccagt tcgcgcacgc
     1201 ctggcagcgc ggcgatccgt tcgagcagcc agacgaggtt gcggcgcacc agcggttcgc
     1261 caccggtgat gcggaccttg ctgacgccga gcgcgacgaa tgtctcgacg atctgcagcg
     1321 actcctccag cgacatgagc gccgagtgtg gcaggaagct cattttttcc gacatgcagt
     1381 aggtgcagcg gaagtcacaa cggtcggtga ccgaaaggcg caggtagctg acgcggcggc
     1441 cgagcgcgtc gatcagcgct gatcgcggcc cggcaagggc aatcgggtcg ggcgcattca
     1501 cggctggtgc tgtccttggg tagtggcttg cggcgcgctg cgcgccgacg agcggagcag
     1561 acaaagacag cgacgctcgc accgatttcc gtgcgaggtc gctgctctgc cagtcacgac
     1621 aggacgccgg aggaggcggg cgccctgtcg atgcgacatc agcccttgtg gagcttgaac
     1681 acccagagcg aaccgccctg gttcagcagg ttcaccttct tcgccacttc gccaccccac
     1741 agcggaacgg cgccacccca gcccgaagcc acggcgatgt actgctcgcc gtcgatctcg
     1801 taggtgatcg gcggggcaac gacgcccgag ccggtctgga agctccagac ttccttgccg
     1861 gtcttggcat cggcagcctt gaggaagccc tccggcgtgc cccagaacac caggccgccc
     1921 ttggtcgtca gcacaccacc ccagagcggc gcgttgttct tcacttccca gacgatcttg
     1981 ccggtggccg ggtcgatcgc tcgcatcgcg ccgatgtagt cgtcgatggt cttgatcgtg
     2041 aagcctgcgc cgaggtaggc cgcgcccttc ttgtagctga ccggctcgtt ccagatgtcc
     2101 atgccccatt cattggccgg cacgtagaag tagccggtat ccgggctgta ggccatcggc
     2161 atctggttct tgccgccgag gaaggagggg gccgcgaaga tcgcttcgcc cttcttgcca
     2221 tcagccgatt tggcgggatc accagggcgg ttggcggggt caaagatcgg cctgccattc
     2281 ttgtcgagtc cctttgccca agtgatcttg ttgacgaagg ggaagccgcg ctcgaacttg
     2341 ccggtcttcg cgtccaagac gtagaagaag ccgttacggt cggccttgcc accgaggatc
     2401 ttgccgctct tgtccttgta ggtgacgaac tcgttcacgc cgtcatagtc ccagccgtcg
     2461 ttgggcgtgc cctggaagtg ccagacgatc ttgccggtct tgacgtcgat ggcgacggtc
     2521 gagcatgagt acaggttgtc gcccgggcga agatgactgt tccacggagc cgggttgccg
     2581 gtcccgaagt aggccagtcc ggtttccttg ttgtagctgc cgccgagcca ggttgccgcg
     2641 ccgccggtct gccagaggtc gccgggccag gtctggttgg tcgtgcccga aacgccgttt
     2701 tccttcttgc cgccgtcttt tgcgtaggta taacccatgt ggccctcgac ggtcggacgg
     2761 acccagacca tcttgccggt cttggcatcg cgtgcctcga cacggccgac gacgccgaat
     2821 tcaccgcccg agacgccggt cagtaccagg ccgttggcga tgatcggtgc cgcggtggcc
     2881 gaatagccgg ccttgtaatc gtcgatcttt tccttccaga cgaccttgcc gctgtcgaca
     2941 tcaagcgcga cgatctgcgc atcgagcgtc atgaagatga acaggttgtc gtagaccgcg
     3001 ccaccacggt tgatcacatc gcagcagggc atgatgccgt cgggcagacg atggtcgtac
     3061 tgccagagct cggcgccggt cttcgcatcg atcgcccagg cgcgcgaata cgagccggtg
     3121 accagtagtt tgccgttaac cagcagcggc tgcgattcct ggccgcgttg tttctcgccg
     3181 ccgaaggaga aggcccaggc cggcacgaga ttcttgaccg tgccggtgtt gacctgggtc
     3241 agcgggctgt agcgctggcc ttcggtgccc atgccccacg acagcacgtc gccggtggtc
     3301 atggcatcct tttcgatatc ggcgtcggtg acgccggcgt aggaactgcc tgtagcggcg
     3361 gcaacgagcg cgccagctac cagtacggac atgaggttct ttcgaaacac gttgcgtctc
     3421 ctttttatgg gaagtgagtt cgcgcgccac gagggtggcc gccgcgtagc cctgtcagca
     3481 agactcgcgc caccgcgtca aacaacgcta cacagggggt gctggggatc gtttcggctg
     3541 gatgggcgct gccgacctgc gtcagcgtga caacactgtc acgcgcacct gtgccaaaaa
     3601 ttaacgcccg ccgcgtcggt tcacggtgcg gggccgaccg cgcgggcttc gagaccgcca
     3661 aagagatggg cgatcgagcg ctccaactct ctgcgcgcca gtggcaggcg ggcgaggtcg
     3721 tcgggcatgg gctgcgccag cacctcggcc gcatcgagtc cgtcggcggc ggcctgacgc
     3781 agcgtctggt cgagccactg cagccagcgc cgggtctgcc gcagtggttt gtcgtcgctc
     3841 gtcggttcgc cgtggccggg aacgagcaga ctgaagggca gcgccgccag cgtatcaagg
     3901 ctgacctgcc agtcgccgat tgccgcgtgc ggcgtcgtcg gtgcgcgatc gtggaaggct
     3961 aggtcgccgg caaagaggac gcccgtcgtt tcgtcgaaga cggcgagatc ggcggcagta
     4021 tggccgctca gcgccagcag ccgaaagcgg tgccggccaa tcgggcggtg accggctgca
     4081 agcatcgttt ccggcgcgtg gatctcggtg cccttggccc agtcgccggt cagtcggtag
     4141 atgttgtcga ggaaggcgcc gccctcggcg cggatgccgc gggccgtttc ggcgagagcg
     4201 ccgatgggca tgtcggcgaa ggcctggttg ccgagaaagt ggtcggggtg atgatgggtg
     4261 atccatatct cgcggatcgg ctcgggcgtg actgtcgcga tcgccgcgcg cagcgcgagg
     4321 ccgtagcggc gcgtcgatcc actgtcgatc acgacgacgc cgtccccggt gtcgatgaag
     4381 gccgtgttga cgatgtcgcc gccgttctgc atcgaaaaat cttcggtggc gccgacgatg
     4441 acccaggtgt cggcggccac ctggcgcggc tgcaggcggt attgcagatc ctcggcggat
     4501 gcgggattcg ccgcggcgac gagcgccagc agccaggaca tggccagcgg tactagtccg
     4561 ggtcgtacgg tcgggcgcgg tcgcacgctc attgcccagg ccccacgttg ctgacttcgg
     4621 cggcgattcg gtttccgttg ttgtcgacgc cgatcagttg cagcggcggg gtcggccggt
     4681 cgaactcgag cgagaacagc ggattttcgg aaagcggttc gaaggtgcgc aggcgaaccc
     4741 agtcccggcc actcgcgtcg cgcagcgcca gccgctcgat gtagaaggcc gggatgcccg
     4801 gcgcgaggcc ggtatccatc gggtgcatga ccttgaactt gagacgccca cgcccctcgc
     4861 gttgccacca gcgggcgtcg acctcgccca gcgtgtcgga ccagttggcc tggctgctgc
     4921 cgacgctggg cgccgtacag ccgccgccgg cggcgtcgac ccaggtgcct ccgacatgcc
     4981 agacgccatc gccggtctgc gccatcgccc gtaccgggct gccctgctgc agcttgatgc
     5041 ggaaggagat gcgcggaacc gcgtcgagcg gttcgaactc gatcaccttg agtatcggat
     5101 tgaggtcggc gaccaccaga acccggcgca cgtcctcgat gtcgcgcagc gacacggtga
     5161 ccggcacgtt catcgaatcc tcggcgaccg cgggcgccag cacgaccacc cgcgtatcga
     5221 aaacgaccgg atcgtcgccg atcagcttgc gccgaacgtc gggccaggac ggcgagtcga
     5281 gcgggtcggc cggctcggcc ggctcggcgt gcacggcgga cgtggcgagg agggcagcgg
     5341 ccagcagcat ggacaaggtt cggcgcatgg tcgatgcatt cttgaggtga tcggggaatc
     5401 aagcaagtgc caagccacgc ctgccggccc gtgcgcggtg ccgatagccc gggctggcgt
     5461 cgcagtccgg gccgcaggtg tgccaatgcg caacaggggg cgtgacacat tggcgcacat
     5521 cagcggcgac cgtcgcccgg gcacccattg cgatgtcacg gcgccgcgcc aatgccctga
     5581 gtgcagcgcc acgtcgttgt ggcccggggc ttgctctatg accccggcgg cattcgccgc
     5641 agcatttgca tccgataaca actgagggaa gagggacaag ggatgaccaa cttcgaaaac
     5701 aatggcgttg cgcggcaggg cctttccgct attcggccac cgttgcgcct gttgccgctg
     5761 gcgctcttgc tcggcacaac ggcgccactg agcgctgcgg aacccgatgg caattcgcgt
     5821 ccggcattgc aggccgcgcc catcgttgtc accgc
//
LOCUS       DATDAA010000071         6331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_40094,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000071 DATDAA010000000
VERSION     DATDAA010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40094"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2461
                     /gene="infB"
                     /locus_tag="VIS73_02260"
     CDS             <1..2461
                     /gene="infB"
                     /locus_tag="VIS73_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY5762008.1"
                     /translation="GVKKAGAGDDVSEADKTRLLDFLRRSHGEAEGKTKITLTRKETS
                     AIKAQGSSGKTRTVQVEVRKKRVLVKREAAVENEPAPVVEPVAPASPVIDETELRNRE
                     EEARRQSELADRQAADIKEKQERELELNRLRQEADEKAAALKVAEAARQQATETAQTA
                     EPADDRTLHKPAGKDAKGKKGGRDVAADDGRRRGGVGLRTRGGGTDRGWRDNRHGKRG
                     GARHDDSAQSFVAPSEPVVRDVHVPETISVADLAHKMSVKATEVIKTLMKMGSMVTIN
                     QSLDQETAMIVVEEMGHKATAAKLDDPDAFLGETVEQKDVAVEPRAPVVTVMGHVDHG
                     KTSLLDHIRRTRVASGEAGGITQHIGAYHVETPRGMVTFLDTPGHEAFTAMRARGAKA
                     TDIVILVVAADDGVMPQTREAIHHAKAAGVPLVVAVNKIDKPEANPERVKQELIAEEV
                     IPEEFGGDSPFVPVSAKTGDGIDDLLEQVLLQAEVLELTAPVDVPAKGLVIEARLDKG
                     RGPVATILVQSGTLKRGDIVIAGQVFGRVRAMLDETGKQIDSAGPSIPVEILGLSEVP
                     NAGDDVQVLLDEKKAREISLFRQGKFREVKLARRANTLETMLEQMGEGDIKHLPLVIK
                     ADVQGSQEALVHALTKLSTGEVKVSVIHAAVGGISESDVNLASASKAVVIGFNTRADA
                     ATRRAAESLGVDIRYYNIIYDAVDEVKAALSGMLSPEKREQVTGLVEIREVYQISRVG
                     AVAGCYVLEGMVKRGSRIRLLRNNVVVHDGELESLKRFKDDVKEVKSNFECGLQLKNF
                     NDIQVGDQLEAYEIQEIARTL"
     gene            2534..2914
                     /gene="rbfA"
                     /locus_tag="VIS73_02265"
     CDS             2534..2914
                     /gene="rbfA"
                     /locus_tag="VIS73_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY5762009.1"
                     /translation="MAQANKGFSRAERVAEQIRRDLAELLSREVKDPRISMVSITAVE
                     LSPDYSHAKIFFTALQPAERRAEIIAGLQAAAGFLRREIGRRIRIHTTPQLHFHYDES
                     IARGADLSQLIDKAAALSRQTPED"
     gene            2965..3837
                     /gene="truB"
                     /locus_tag="VIS73_02270"
     CDS             2965..3837
                     /gene="truB"
                     /locus_tag="VIS73_02270"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY5762010.1"
                     /translation="MLLDKPTGMTSNDALQKARRLYSAEKGGHTGTLDPMASGLLPVC
                     FGEATKFGSDLLDADKTYLATLRLGERSNTGDADGEIVERRPVTVDDATVREALAALL
                     GPQMQTPPMHSALKRNGVPLYRLAREGIEVEREPRAIRIDAIAAVRIALPELVIRVAC
                     SKGTYIRVLAEDIGEALNCGAHLTALRRERVAALSIDEALSLDALSALDDAARLAVLR
                     PVDFLLQELPRIEIDEAALQRFGNGNPICVKPGELDQGDRFRVYSGGRLIGLGRRDDE
                     GHLQPQRLLRRDPT"
     gene            3937..5574
                     /locus_tag="VIS73_02275"
     CDS             3937..5574
                     /locus_tag="VIS73_02275"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY5762011.1"
                     /translation="MTKFVFVTGGVVSSLGKGIAAASLGAILESRGIRVTHLKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTSAKMGKRNNFTTGQIYESVIKKE
                     RRGEYLGKTVQVIPHITDEIKIFVKRGAEGADVAIVEVGGTVGDIESLPFLEAIRQMG
                     IEEGRSGCCFVHLTLLPYIPTAGELKTKPTQHSVKELREIGIQPDVLLCRADRPIPVD
                     ERRKIALFCNVMPEAVIEALDADSIYKIPAMLHAQMLDQIVCHKLDLLARPANLAVWS
                     KLVEALENPQHEVNIGFVGKYVDLTESYKSLIEALVHAGMHTRSKINIRYLDSETIEK
                     QGCGVLSELHAILVPGGFGKRGTEGKIAAIRYARENRIPYLGICLGMQLATIEFARHV
                     AGLEGANSTELDATTPHPVVALITEWLDREGRIEKRDAASNLGGTMRLGSQRCPVAPK
                     TLAAEIYGTEVNERHRHRYEVNNAYVPRLEASGLVISARTPNENLPEIMELPREVHPW
                     FIGVQFHPEFTSNPRNGHPLFIAYVKAALAAQAAKGT"
     gene            5576..>6331
                     /gene="kdsA"
                     /locus_tag="VIS73_02280"
     CDS             5576..>6331
                     /gene="kdsA"
                     /locus_tag="VIS73_02280"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY5762012.1"
                     /translation="MKLCGFEAGLEHPFFLIAGPCTAESEQLCLDVAGAMKEICTPLG
                     IPYIFKASYDKANRSSGTSPRGPGVDRGLEWLARVRREIGVPVLTDVHTIDEIAAVAA
                     VVDVLQTPAFLCRQTDFIHAVAATGKPVNIKKGQFLAPGDMKHVVSKAKEANGGADTI
                     MVCERGASFGYNNLVSDMRSLAIMRETGCPVVFDATHSVQLPGGQGTRSGGQREFVPV
                     LSRAAVGAGVAGLFMETHPTPEKAFSDGPNAWPL"
BASE COUNT         1221 a         2000 c         2058 g         1052 t
ORIGIN      
        1 cggcgtcaag aaggccggtg ccggcgacga cgtcagcgag gcggacaaga cgcgcctgct
       61 cgacttcctg cgccgctcgc acggcgaggc cgagggcaag accaagatca cgctgacccg
      121 caaggagacc agcgcgatta aggcgcaggg ttcgtcgggc aagacgcgga cggtacaggt
      181 cgaagtccgc aagaagcgcg ttctcgtcaa acgcgaagct gcggtcgaga acgaaccggc
      241 gccggttgtc gagccggttg cgccggcatc accggtcatc gatgaaaccg aactgcgaaa
      301 tcgcgaggaa gaggcccggc gccagagcga actggctgac cggcaggcag ccgatatcaa
      361 ggaaaagcag gagcgcgagc tcgagctcaa tcgcctgcgc caggaggccg acgagaaggc
      421 cgcagcgctg aaggtcgcgg aagcggcccg tcagcaggcg accgaaaccg cccagacggc
      481 agagccggcc gacgatcgca cgctgcacaa gccggcaggc aaggatgcca agggcaagaa
      541 gggtggccgt gatgtggcgg ccgatgatgg acgccgccgc ggcggcgtcg gtctgcgcac
      601 gcgcggtggc ggcaccgatc gcggctggcg cgacaaccgt cacggtaagc gtggtggcgc
      661 ccgtcacgac gattcggcgc aatccttcgt cgcgccgagc gaacccgttg tccgcgacgt
      721 gcacgtgccg gaaaccattt cggttgccga cctcgcgcac aagatgtcgg tgaaggcaac
      781 cgaagtcatc aagaccctga tgaagatggg gtcgatggtt acgatcaacc agagcctcga
      841 tcaggagacg gccatgattg tcgtcgaaga gatggggcac aaggcgaccg cggccaagct
      901 cgacgacccc gatgccttcc tgggagaaac cgtcgagcag aaggacgttg cggtcgaacc
      961 gcgggcgccg gtggtgaccg tgatgggcca cgtcgaccac ggcaagacct cgctgcttga
     1021 tcacatccgt cgtacgcgcg tcgcttcggg cgaggcgggg ggcattaccc agcacatcgg
     1081 cgcctatcac gtcgaaacgc cgcgcggcat ggtgaccttc cttgatacgc cgggccacga
     1141 ggcatttacc gcgatgcgtg cgcgtggcgc caaggcgacc gacatcgtca tcctagtggt
     1201 tgccgccgac gacggcgtca tgccgcagac gcgtgaggcg atccatcacg ccaaggcagc
     1261 cggggttccg ctggttgttg ccgtgaacaa gatcgacaag cccgaagcca accccgagcg
     1321 ggtcaagcag gaactgatcg ccgaagaggt catccccgag gaatttggcg gcgactcgcc
     1381 tttcgtcccg gtttcggcga agaccggcga tggcatcgac gacctcctcg agcaggtact
     1441 gctgcaggct gaagtcctcg aactcaccgc accagtcgat gtgccggcca agggcctggt
     1501 gatcgaggcg cgtcttgaca agggtcgcgg cccggtggcg acgattctcg tgcagagcgg
     1561 cacgctcaag cgcggcgaca tcgtcattgc cggccaggtc tttggtcgcg ttcgcgcgat
     1621 gctcgacgaa accggcaagc agatcgattc tgccgggccg tcgattccgg tcgagattct
     1681 cggtctctcc gaagtgccga acgccggtga cgacgtacag gtactgctcg acgagaagaa
     1741 ggcgcgcgag atctcgctgt tccgtcaggg caagttccgt gaggtcaagc tggcacgccg
     1801 cgccaatacg ctcgagacca tgcttgagca gatgggcgaa ggcgatatca agcacctgcc
     1861 gctggtgatc aaggccgacg tgcagggttc gcaggaagca ctcgtgcacg cgctgaccaa
     1921 gctgtcgacc ggcgaggtca aggtcagcgt aatccacgcg gccgtgggcg gcatcagcga
     1981 atccgacgtg aacctggcgt cggcctcgaa ggccgttgtg atcggtttca acacccgtgc
     2041 cgatgcggca acgcgccgcg ctgccgaatc gctgggcgtc gacatccgct attacaacat
     2101 catctacgac gcggtcgacg aggtgaaggc tgccctctcc ggcatgctct cgcccgagaa
     2161 gcgcgagcag gtcaccggcc tggtcgagat tcgggaggtc taccagatct cgcgcgtcgg
     2221 tgcggtggcc ggctgctacg tgctcgaagg catggtcaag cgcggttcgc ggatccgcct
     2281 gctgcgcaac aacgtggtcg tccacgatgg cgaactggaa tcgctaaagc gattcaagga
     2341 cgacgtcaag gaagtgaagt cgaacttcga gtgcggtctg caactgaaga acttcaacga
     2401 cattcaggtc ggagaccaac ttgaggcata cgaaatccag gaaatcgccc ggacgctctg
     2461 atcgaccgcg ggcggcggaa acgccgccgc ggccgggcga acggtgtccg acggcccgcc
     2521 gaggagaagc gacatggcgc aagccaacaa gggattttcg cgagccgagc gcgtcgccga
     2581 gcagatccga cgtgatctgg ccgagttgct gagtcgtgag gtcaaggacc cgcggatcag
     2641 catggtcagc attaccgcag tcgagctgtc acccgattac tcacacgcca agattttttt
     2701 cacggcgctg cagccagccg agcggcgtgc ggaaatcatc gccggcctgc aggccgccgc
     2761 cggtttcctg cgccgtgaga tcggtcgtcg gatccgcatt cacaccacgc cgcaactgca
     2821 cttccattac gacgagtcga ttgcccgcgg cgccgacctg agccagctga tcgacaaggc
     2881 cgccgcgctg tcgcggcaga cgcccgaaga ctgaccttcc gcaagaatgt agcgaagcga
     2941 cgctggcaac aggtcgatgg cgtcctgctg ctcgacaagc cgaccgggat gacctcgaac
     3001 gacgccctgc agaaggctcg tcgcctgtac tcggccgaga agggcggaca caccggaacg
     3061 ctggatccga tggccagcgg gctgttgccg gtctgctttg gcgaggcgac gaagttcggt
     3121 agcgacctgc tcgatgccga caagacctac ctagcgacgc tgcgcctcgg cgagcgcagc
     3181 aacactggcg acgccgacgg cgagatcgtc gagcggcgcc cggtcaccgt cgacgacgcg
     3241 accgttcgtg aagccctggc ggcgctcctt gggccgcaga tgcagacgcc gccgatgcat
     3301 tcggcactca agcgcaacgg cgtgccgctc taccggctgg cgcgcgaagg catcgaggtc
     3361 gagcgtgagc cgcgcgcgat tcgcatcgat gccatcgcgg ccgtccggat tgcgcttccc
     3421 gaactggtga tccgcgtcgc ctgcagcaag ggcacctata ttcgggtgct tgccgaagac
     3481 atcggcgagg cgctgaactg tggcgcacat ctgacggcct tgcgccggga gcgcgtcgcc
     3541 gccttgtcga tcgacgaggc attgagcctc gatgcgctgt cggcgctgga cgacgcggcg
     3601 cggctcgccg tgctcagacc ggtcgacttt ctgctgcagg aactgccgcg tatcgaaatc
     3661 gatgaggccg ctttgcagcg cttcggcaat ggcaacccga tctgcgtcaa acccggcgag
     3721 cttgaccagg gagaccggtt ccgcgtctat tctggcggac gtctgatcgg tctcggccgg
     3781 cgcgacgacg agggtcacct gcagccgcaa cggctgctgc gacgcgatcc gacatgatcg
     3841 cctgatcggg gaccgattgc tgccgacgag tcgattctgg gctttggcgg cacggcagtt
     3901 tcggctagaa ttcggctttc ccgcgccgga cccctcatga ccaagtttgt cttcgtaacc
     3961 ggcggtgtcg tgtcctccct ggggaagggc atcgccgcgg cgtctctcgg tgccattctc
     4021 gaatcccgtg gtatccgcgt cacccatctg aagctcgacc cgtacatcaa cgtcgatccg
     4081 gggacgatga gcccgtttca gcatggcgaa gtcttcgtca ccgaagacgg tgccgagacc
     4141 gatctcgatc tcggccacta tgagcgcttc accagcgcca agatgggcaa gcgcaacaac
     4201 ttcaccaccg gccagatcta cgagtcggta atcaagaagg agcggcgcgg cgaatatctg
     4261 ggcaaaacgg ttcaggtcat tccgcacatc accgacgaga tcaagatctt cgtcaagcgt
     4321 ggcgccgaag gcgccgacgt ggcgatcgtc gaagtcggcg gcaccgtcgg tgacatcgaa
     4381 tcgctgccct tcctcgaggc gatccgccag atgggcattg aggaggggcg aagcggctgt
     4441 tgctttgtcc acctgacgct gttgccctac atcccgaccg ccggcgaact gaagaccaag
     4501 ccgacccagc actcggtgaa agagttgcgc gagatcggca tccagcccga cgtgctgctc
     4561 tgccgtgccg atcgcccgat tccggtcgac gagcggcgca agatcgcctt gttctgcaac
     4621 gtcatgcccg aggcggtgat cgaggcgctc gatgccgatt cgatctacaa gattccggcg
     4681 atgctgcacg cgcagatgct cgaccagatt gtctgccaca aactcgatct cctcgcccgc
     4741 ccagccaatc tggcggtctg gtcgaaactc gtcgaggcat tggagaaccc gcaacacgag
     4801 gtcaacatcg gttttgtcgg caagtacgtc gatctcaccg agtcgtacaa gtcgctgatc
     4861 gaggcgctgg tgcatgcagg catgcatacg cgcagcaaga tcaacatccg ctacctcgat
     4921 tcggaaacga tcgagaagca gggatgcggg gtgctttccg agctgcatgc gatcctggtt
     4981 ccgggcggct tcggcaaacg tggaaccgaa ggcaagatcg cggcgattcg ctatgcgcgc
     5041 gagaaccgga ttccctacct cggcatctgc ctcggcatgc agctcgcgac gatcgaattt
     5101 gcccgccacg tcgctggcct tgagggagcc aacagcactg agctggatgc cacgacgccg
     5161 catccggtcg tggcgctgat caccgaatgg cttgaccgtg aagggcggat cgagaagcgc
     5221 gatgccgcgt cgaacctcgg tggcacgatg cggcttggct cgcagcgctg cccggtcgcg
     5281 ccgaagacgc tggccgccga aatctacggt accgaggtca acgagcgcca ccgccatcgc
     5341 tacgaggtca acaacgccta tgtgccgcgc cttgaggcgt cgggtctggt catttcggcg
     5401 cggacgccga acgagaacct gcccgaaatc atggaactcc cgcgcgaggt gcatccctgg
     5461 ttcatcggcg tgcagttcca ccccgagttc acctcgaatc cgcgcaacgg gcatccgttg
     5521 ttcatcgcct acgtcaaggc ggcgcttgcc gcgcaagccg cgaaggggac ttgagatgaa
     5581 actctgcgga ttcgaagccg gcctagagca tcccttcttc ctgattgccg gtccatgcac
     5641 cgcagagtcc gaacaactct gcctcgacgt tgccggcgcg atgaaggaaa tctgtacgcc
     5701 gcttggaatc ccgtacatct tcaaggcctc ctacgacaag gccaaccgca gttccggcac
     5761 gtcgccgcgt gggcccggcg ttgaccgtgg tctggaatgg ctggcgcgcg tgcgccgcga
     5821 gatcggtgtg cccgttctga cagacgtgca cacgatcgat gagattgccg ccgttgccgc
     5881 cgttgtcgat gttctgcaga cgccggcctt cctctgccgg caaaccgact tcattcatgc
     5941 tgttgctgcg accggcaagc ctgtcaacat caagaagggt cagttcctcg cgcccggcga
     6001 catgaagcac gtggtgagca aagcgaagga agccaatggc ggcgccgata cgatcatggt
     6061 ctgcgaacgc ggcgcgagct tcggctacaa caatctggtt tccgacatgc gctcgttggc
     6121 gatcatgcgc gagaccggct gtccggttgt tttcgacgcg acccactcgg tccagttgcc
     6181 cggtggacag gggacgcgtt cgggcgggca gcgcgaattc gttccggtgc tctcgcgtgc
     6241 cgcagtcggc gccggtgtcg ccgggctctt catggaaacg catccgacgc ccgagaaggc
     6301 cttttccgat ggcccgaatg cctggccgct g
//
LOCUS       DATDAA010000072        23809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_459692,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000072 DATDAA010000000
VERSION     DATDAA010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 23809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23809
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459692"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VIS73_02285"
     CDS             complement(<1..699)
                     /locus_tag="VIS73_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEY5762013.1"
                     /translation="MKKANPKSTEQLSRKRLADPFLERESARYEFPLPSREYIRQTLE
                     ALGRPADFVELCEALDIREDEELMFQRRLGAMAREAELMQNRRGAFIVPEKAQLIPGR
                     VHGHKDGYGFLIPDAGGDDLFLGPRQMEKVLHGDRVIGRIVGLDRRGRPEGVIVEVTE
                     RGATKIVGRVLVEHGVVFVVPEDRRVSQDILIPPDALAKPIPQSGQVVVAEITEPPTR
                     YSKPIGRIVEVLGNY"
     gene            746..832
                     /locus_tag="VIS73_02290"
     tRNA            746..832
                     /locus_tag="VIS73_02290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:780..782,aa:Leu,seq:cag)
     gene            complement(884..2194)
                     /locus_tag="VIS73_02295"
     CDS             complement(884..2194)
                     /locus_tag="VIS73_02295"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY5762014.1"
                     /translation="MAKNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTL
                     VIGTREYKLNLVPSGIVREGVRCYIGNGVVLDIHHLLEEIRNLEAGGLKVSERLRISP
                     GCPIILGHHVALDRAREAAKSAGDKIGTTGKGIGPTYEDKVARRALRVYDLFDKERFA
                     TKLRELLDYHNFVLTKFLKAEPVEFQKVYDQAMADAEAIKPMVEDVSAALYAANKAGH
                     NLLFEGAQGTLLDIDHGTYPYVTSSNCVAGQAAAGSGVGPTRLHYILGITKAYCTRVG
                     GGPFPSELDIETAGTPGEVMSRVGREFGTVTGRKRRCGWLDLAALKRSIIINGVTGLC
                     ITKLDVLDGLPELKLGLGYELDGEKIDLLPMGADEVARCKPIYETMKGWGGTTFGAKR
                     WDDLPPEARAYLHRIEEICEIPIDVISTGPERDETILRRHPFGA"
     gene            complement(2217..3362)
                     /locus_tag="VIS73_02300"
     CDS             complement(2217..3362)
                     /locus_tag="VIS73_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY5762015.1"
                     /translation="MNWLLPEYIADALPREAAHIERVRRACLDQLRTHGYLLVMPPLL
                     EYLESLFTGAGRDLRLRTFELTDQLSGRTMGVRADMTPQITRIDAHLLNHAGVTRLCY
                     CDSVLHALPASATASREPIQLGAELYGYPGCEADIEVVRLMAATLECGGLADFRLDIG
                     HVGIFRALTSAAGIAGAEEEALFAIMQNKDLPALAEALAKCGDPWRSALLRLPELFGG
                     PPVLAHARAALPDSPAIAEALDVLDRLAAGVAGRAPSFDLADLRGYHYHNGVVFAAYC
                     AGFAGPIALGGRYDNVGAAFGRARPATGFSLDLRELARLTVSDTAASAVLAPAGSDAR
                     LLARIAELRAAGEIVVQCLPGEAQPELPRCDRELVCRNGDWMIANAH"
     gene            complement(3359..3544)
                     /locus_tag="VIS73_02305"
     CDS             complement(3359..3544)
                     /locus_tag="VIS73_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEY5762016.1"
                     /translation="MIDNLLAALALMLVIEGVLPFVAPGTWRETFKRLVAMSDGQVRF
                     IGLTSMIVGLFMLLVFL"
     gene            complement(3569..4438)
                     /gene="hflC"
                     /locus_tag="VIS73_02310"
     CDS             complement(3569..4438)
                     /gene="hflC"
                     /locus_tag="VIS73_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEY5762017.1"
                     /translation="MANRLKFVLAFLVAGILLASVTLFTVDQRQFAIVFQLGEVKTVI
                     DKPGLHFKFPFVQNVRFFEKRILTLDSPEPDRFITSEKKNVLVDSFVKWRIIDPRLYY
                     ISVQGDETRARTRLSQTVNAGLREEFGKRTVNDVVSGERDKIMDEMRIKADLDARKIG
                     VQIVDVRLKRVELPDEVSVSVYRRMEAERKRVANELRSTGFAESEKIRADADRQREVI
                     IANAYRESQQIKGDGDARATAIYARAFGQNPEFYDFYRSLEAYRESFRNKSDVLVVDP
                     SSDFFKYLKKSGK"
     gene            complement(4440..5654)
                     /gene="hflK"
                     /locus_tag="VIS73_02315"
     CDS             complement(4440..5654)
                     /gene="hflK"
                     /locus_tag="VIS73_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEY5762018.1"
                     /translation="MSMNDPEWGNRGNGGRRPNQGPPDLEELWGDLNRRLAGILGKRG
                     GGGGSSGGGGNRGADIDPKLFGGGVGIVLFLIAAAWAASGFYIVDESQRAVVLRFGKV
                     TEVTDPGLRWRLPFPIESHELVNLSGVRTAEIGYRGSEKNKVLAEALMLTQDENIINI
                     QFAVQWFLKDPLEYVINNRNPDEAVTQAAETAIREIVGKSNMDYVLYEGREQVAQQAA
                     VLMQDILDRYKTGIIVSKFTMQNAQPPEQVQAAFDDAVKAGQDRERQKNEGEGYANDV
                     IPKARGSAARLLEEASAYKQRVVATAEGDASRFRQIYTEYSKAPEVTRKRMYLETMQE
                     VFANTGKIMLDTKGSGNLLYLPLDQLMRSAGAIAAAESAAAAAAKVQEPAVADPAPPR
                     RDSLRSRNREGR"
     gene            complement(5678..6862)
                     /gene="hflX"
                     /locus_tag="VIS73_02320"
     CDS             complement(5678..6862)
                     /gene="hflX"
                     /locus_tag="VIS73_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY5762019.1"
                     /translation="MFERPASGENVVVVQFDIGRVEDYERAEQLSEIRLLTQSAGGRI
                     VGEMLGKRAAPDAATFAGKGKVAELAAQVAGAGAELVVFNHMLSPAQQRNLERALNCR
                     VVDRTSLILDIFALRASSAEGKMQVELAQLEHLATRLVRGWTHLERQRGGIGLRGPGE
                     KQLETDRRLLGNRVKILKQRLARLDRQRGTQRRARARGSVLNVSLVGYTNAGKSTLFN
                     AMTKAGVFAANQLFATLDTTSRKVFLEPGLHAVVSDTVGFIRELPHALVEAFKATLEA
                     TAQADLLIHVVDSSAPDRDDQIAAVDGVLAEIGADDVPQVVVLNKCDLTALPPGVEVD
                     AYDRISRVRLSALNGAGLDLLRDALAERAKEKRREFAQSTSQSDEELPSGEPDVAHVD
                     GL"
     gene            complement(6884..7120)
                     /gene="hfq"
                     /locus_tag="VIS73_02325"
     CDS             complement(6884..7120)
                     /gene="hfq"
                     /locus_tag="VIS73_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY5762020.1"
                     /translation="MSNKGQLLQDPFLNTLRREHIPVSIYLVNGIKLQGQVESFDQYV
                     VLLKNTVTQMVYKHAISTVVPARPVTIQQEAGAD"
     gene            complement(7249..8577)
                     /gene="der"
                     /locus_tag="VIS73_02330"
     CDS             complement(7249..8577)
                     /gene="der"
                     /locus_tag="VIS73_02330"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY5762021.1"
                     /translation="MIPTVVLVGRANVGKSTLFNRLTKSRNALVADLPGLTRDRHYGH
                     GRIGSKPFLVVDTGGFEPLAKDGIVYEMARQTEQAIAEADLVVFLVDGRQGVAPLDKE
                     IANKLRRLDVPVVVAVNKAEGFDPGVAAADFHSLGLGEPQPISAAHGEGVRHLIEDAL
                     AEWPDSTGEPANESPRIAIVGRPNVGKSTMINALLGEERVIAFDMPGTTRDAIEIPFT
                     RGERQYTLIDTAGLRRRGKVFEAIEKFSVVKTLQSIEQANVVVLVLDAQQDISDQDAH
                     IAGFVVESGRALVVAVNKWDGIDDYRRTQIKAEIARRLQFLDFAKHHFVSALEGQGLT
                     ALMRSVDGAYAAAMAKMATPRLTRTLTEAVARQAPPRHGIFRPKMRYAHQGGNNPPII
                     VIHGTALDHVPDSYRRYLEHVFRDAFKLQGTPLRIQFNVAKNPYAEKPRR"
     gene            complement(8580..9680)
                     /gene="bamB"
                     /locus_tag="VIS73_02335"
     CDS             complement(8580..9680)
                     /gene="bamB"
                     /locus_tag="VIS73_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HEY5762022.1"
                     /translation="MAVAVALLASGCASEPSRKPLELPEITAETSIRVVWQVPGGDLG
                     HAALRPAANADAVFIAAPDGRLGRYRAGEPVWEIATKLPISGGVSTDGEIVVVGTSKG
                     EVVAFATNDGSERWRARVSSEVLAPPVFAGDRILVRSGDHRLFALDARDGTRQWVMQR
                     TTPALTLRVTTPPVFAENFAFLGYPGGKLLAVGVANGAVLWEGSVALSKGTTELERIA
                     DIAGSPVLGPREVCAAAYQGRVACFDLSSGNLIWAQEVSADSSIALDSSNIYVADTDG
                     VVHAYARADGKLVWQQDALKWRRLGAPLVRRGFLLVGDGSGVLHALRLSDGALVGRLA
                     SDGGGFSSDPVIAGDTTIYQTRRGGVLAVELD"
     gene            complement(9710..10360)
                     /locus_tag="VIS73_02340"
     CDS             complement(9710..10360)
                     /locus_tag="VIS73_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5762023.1"
                     /translation="MATYDLEEQEQLDSLKAWWRQYGDWITGVLLVVSLSFAGYQGWN
                     WYQRNQSAEASALFASLQQAANLEEDQRVRAAAGELLEKYSSTRYAEMGAMISARVAF
                     EAGDRKTAQAQLQWVVDHADSELRDLARLRLTAVLLEDGNVDAALATVAKPDQASFAP
                     RFAEVRGDILLVQGKRAEARTAYEAALAAMPTETDASAQRYRELLKQKRDALAAVK"
     gene            complement(10362..11666)
                     /gene="hisS"
                     /locus_tag="VIS73_02345"
     CDS             complement(10362..11666)
                     /gene="hisS"
                     /locus_tag="VIS73_02345"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY5762024.1"
                     /translation="MTQQLQSVRGMNDILPDEADLWERFDEVVRDWLRAYGYRPIRLP
                     IVEPTPLFTRAIGAVTDIVEKEMYSFEDSLNAEKLTLRPEGTAGCVRAVIQHGLLGRG
                     PQRLYYTGPMFRHERPQKGRYRQFYQIGVEALGLAGPDIDAEMILMGARLWDDLGLDG
                     IRLQINSLGQADERARHRSDLIAYLESNTDRLDAEATRRLHSNPLRILDSKNPEMQEM
                     IAGAPRLIDYLGPESLAHFEAVQQVLKDQGLPYEINPRLVRGLDYYNLTVFEWVTDRL
                     GAQGTVCAGGRYDGLIEQLGGKPAPACGFAMGVERLIALIQDAGGAPDAPVPDVYVVH
                     QGEAARRSAYRAAESLRNVGFDVLMHAGGGSFKSQMKKADGSGASYAVIIGDDEAAAD
                     EVTLKALRADGTEGGTGGPQRRVAVATLADELTDEMIDWEEN"
     gene            complement(11663..12892)
                     /gene="ispG"
                     /locus_tag="VIS73_02350"
                     /gene_synonym="gcpE"
     CDS             complement(11663..12892)
                     /gene="ispG"
                     /locus_tag="VIS73_02350"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY5762025.1"
                     /translation="MNMLARRVTRAVRVGSVLIGGGNPVVVQSMTNTDTADVIGTARQ
                     VVELAQAGSELVRITVNTPEAAAAVPRIQEQMSRLGCEVPLIGDFHYNGHRLLADFPD
                     CASTLAKYRINPGNVGFGARRDEQFARMIEIACRLDKPVRIGVNWGSLDQGVLARVMD
                     ENARRPEPADAGAVMREAMVSSAIESAARAVEIGLPAERIVLSCKVSSVQDLIAIYRM
                     LAERTDLPLHLGLTEAGIGSKGIVASCASMGVLLQEGIGDTIRVSLTPEPGEPRTGEV
                     RVAQELLQTMGLRAFTPLVTACPGCGRTTSTYFQELASEVQDFVRARMPAWRSEYDGV
                     EHMTLAVMGCVVNGPGESKHANIGISLPGTGEAPAAPIFVDGEKTVTLRGERITEEFK
                     ALIERYVTTKYVRKGGA"
     gene            complement(12889..13923)
                     /locus_tag="VIS73_02355"
     CDS             complement(12889..13923)
                     /locus_tag="VIS73_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEY5762026.1"
                     /translation="MSENRQSELPGTDKVIEVEMSPSPSPSPSPSPSPSPGAQLRAAR
                     EVAGISIADAAQSIRLSPRQIGLLESDAWDQLPGETFVRGFLRNYARLLKVDPEPLLA
                     SVSAPTPQPIELPEGSHAEMPAEGSSSSQLMPMLAGGGLLLVAAIAAYFFWPIESAPN
                     VVPPKRPLAPPTAESVPAPPMVSPGPAAPAAAVADPVATAPAVVVEPPSVPAANPAAT
                     AVPVVAEKPAPSVRAPEPLAPAASQPAKPAEAAAAPVAPRPFVVVDGPQRALRFTVRE
                     LSWVEVHDAGGNTLIAYNFEPGTSRNVRGVAPLEVVVGNASGVDLSVDGEAVDLAARA
                     RANVAKLTVE"
     gene            complement(13920..14723)
                     /gene="pilW"
                     /locus_tag="VIS73_02360"
     CDS             complement(13920..14723)
                     /gene="pilW"
                     /locus_tag="VIS73_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEY5762027.1"
                     /translation="MNPVRRLAMIPLLCVGLLLAGCAGSGMSTAERAVEPSSQAALSS
                     AKTQRARAHIELGTAYFQAGNMAVALEEVRIALSADRSYAPAYNLSGLIHMYLRENPV
                     AEENFREGLRLAPGDPEISNNYGWFLCQTGREADGIAQFVSAVKNPLYATPELSLTNA
                     GICAMQLGDLAAAEDYLIKALRLGRNTGGALLQLGHLNYRQKRYVDARDYLAKLHAQV
                     EPTAESLWLAIRVERAAGDRRAEAGFISQLRRRFPDSKEARESRQGVGG"
     gene            complement(14720..15811)
                     /gene="rlmN"
                     /locus_tag="VIS73_02365"
     CDS             complement(14720..15811)
                     /gene="rlmN"
                     /locus_tag="VIS73_02365"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY5762028.1"
                     /translation="MNDQAANLLDFDHESLKAFFLARGEKAFRATQVLRWVHQAGASD
                     FAAMTDLARSLRAWLAESAVVAPPPLISDRLSDDGTRKFLFDVGSGNAIESVFIPEDD
                     RGTLCVSTQAGCALDCAFCSTGKQGFNRNLTTAEIIGQLWQANALIGRGSGGERPVSN
                     VVMMGMGEPLANFDATVSALRLMLDDHAYGLSRRRVTVSTAGLVPAIDRLAEACPVAL
                     AVSLHAPDDALRDQLVPINRRYPLAELLAACRRYLNAAPRDFITFEYVLLEGVNDSDA
                     HARKLVEIAGEVPCKYNLIPFNPFPGSPFRRPSPARVKAFAALIQQAGIVTTTRKTRG
                     DDIDAACGQLAGQVLDRTRRTSRTVEFHR"
     gene            complement(15881..16306)
                     /gene="ndk"
                     /locus_tag="VIS73_02370"
     CDS             complement(15881..16306)
                     /gene="ndk"
                     /locus_tag="VIS73_02370"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY5762029.1"
                     /translation="MAVERTLSIIKPDAVAKNVIGKIYSRFETNGLKVIAAKMAHLST
                     IEAGKFYWVHRERPFYNDLVKFMTSGPVMIQVLEGEGAIAKNRDLMGATDPKKAAAGT
                     IRADFADSIDANAVHGSDAPETAAVEIAFFFPEMNVYSR"
     gene            complement(16474..17757)
                     /locus_tag="VIS73_02375"
     CDS             complement(16474..17757)
                     /locus_tag="VIS73_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY5762030.1"
                     /translation="MTTLVIFGLLITLMAVGMPVGVALGLTVLTFMWAFTSTPIEAVA
                     LKMFTGIEKFEIMAIPFFILAGNFLTHGGVARRMINFATAMVGHMTGGLGMSAVLACA
                     IFAAVSGSSPATVVAIGSILIPAMLKQGFPLRFSAGVVASAGGLGILIPPSIVMVMYA
                     VSTDSSIGALFMAGVIPGIMLAFMLGFTTWWQAKKHNYPRQPKASWKERWTYFREAVW
                     GLMLIVVVMGGIYSGMFTPTEAAAMSAVYAFFIAMFVYKDIGFRQVPRVLLDAANMSS
                     MLLFIIASAVLFSFILTSEQIPQRMAEAIVASGLGPVGFLVVVNVLLLLAGNIMEPSS
                     IILILAPILFPVATALGIDPVHFGIIITVNMEIGMITPPVGLNLFVASGITKAGMTEM
                     TLAVLPWLYTMLVFLILITYVPAISLWLPKTLGMM"
     gene            complement(17761..18474)
                     /locus_tag="VIS73_02380"
     CDS             complement(17761..18474)
                     /locus_tag="VIS73_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY5762031.1"
                     /translation="MNKVIDRLEEFIIGSLMALATIIIFISVVHRYGSGFAIPGVQDW
                     LLSIHMGWAQELCLILFVWMAKFGAAYGVRTGIHVGVDLLINKLEGKPQEVLIQIGLF
                     AGVFFTGVIAVFGAVFVWENGMAYETLTALGRDAGDYFEGPITPDLEWPTWRVYAAIP
                     LGSGLMCFRFIQAMIRFHKTGELPHHDHGHVDGIEEPAPDMLGEAIEAFEEREHAHHG
                     KSDAEWEKTHPKNNGKQEK"
     gene            complement(18608..19609)
                     /locus_tag="VIS73_02385"
     CDS             complement(18608..19609)
                     /locus_tag="VIS73_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY5762032.1"
                     /translation="MRISTLLCGMLLGVFAVTASAQEIVVKFSHVVADSTPKGKGAIK
                     FKELAEQYTNGKVKVEVYANSTLYKDKEEMEALQLGAVQMIAPSLAKFGPLGVRTFEV
                     FDLPYMFDGYADLHKVTEGPLGQKFLTSLEPKGIKGLAFWDNGFKVMSSNKPIRTPAD
                     YKGQKLRIQSSKVLESQMRALGALPQVMAFSEVYQALQTGVVDGTENPPSNLYTQKMH
                     EVQKYVVESNHGYLGYAVITNKKFWDGLPADIRGQLEKAMVDATKFANGIAKEENEMA
                     MAEVAKAGKSEIIKLTADEKKAMKKALVPVHKEMESRIGADLIEAVYKETGFDPSKL"
     gene            19842..21758
                     /locus_tag="VIS73_02390"
     CDS             19842..21758
                     /locus_tag="VIS73_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5762033.1"
                     /translation="MPNIAIALLVVAVIGLLWVLQEQNREDRRATLIADILWLEQNLR
                     FELVGVRDRLSQLANALPAEANPREFFDQHIRQLRQGNAEIVQVIWLDETGRVYRANP
                     APTHPHLIGGAIADQLLSDRIEVARQLGRRVYTAPYSLDDSGAQFEIVVPLFRDGQYA
                     GALIGVHSLQSMLDHSVPWWFTEKYQLEVINNEGQIIGSKSNIVGKPQSTYNLVLDEL
                     GQGVTLRAGLTRSDTDRAARFVVAAILVLAAAVFWSLWAIRGLIRRRLRAEEQLRGAN
                     AFRESMENSLLTGLRARDLDGRLIYANPAFCRMVGFSETELMGHLPPMPYWDPGSVEE
                     TMRIHQEVLHGHAPPDGFEMRFRRKNGEGFDALVYEAPLIDGHGEHVGWMGSVLDITE
                     RKRIAEFKRQQEDKLQHTARLVAMGEMASTLAHELNQPLAAIASYTAGCLNKLESGRH
                     TEAELTTVLRKINAQSQRAGKIIRQVHDFVRRREPRREACAVAEVVDEAVTLFEAQAK
                     KHGVRIACSLQGGLPEVSADPTMLEQVVINLLRNAVEAMADSPAEERRVDVSVRREGD
                     RIVVRVADNGPGIPPGSMEMLFTPFFTTKPEGMGMGMAICRSIIEFHHGRLDARNSEA
                     GGAEISFFLPIIPQ"
     gene            21755..22357
                     /locus_tag="VIS73_02395"
     CDS             21755..22357
                     /locus_tag="VIS73_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5762034.1"
                     /translation="MKALAHIIDDDEAIRDSLEWLLESRNIPAQSWPSAESFLAHFDA
                     CRGSTPGCLLLDIRMDGMSGLALFDLLCERGDRTPVIFLTGHGDVPMAVGALKRGAFD
                     FVEKPFDDNELVARITEAMAIDAAKRAASAVSASVADSLATLTQRERQVMELVLAGKL
                     NKIIADELSISMRTVEVHRARVFEKMGVKSAVELAQKLMR"
     gene            complement(22371..23756)
                     /gene="tilS"
                     /locus_tag="VIS73_02400"
     CDS             complement(22371..23756)
                     /gene="tilS"
                     /locus_tag="VIS73_02400"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY5762035.1"
                     /translation="MVGSRSSGSPNTRSGACPVLAAVRAALRDIPEGCRLSVGYSGGL
                     DSTVLLHALTQVGGEPSRSLSAIHVHHGINPQADAWAAHCRSVCAGLGVPLRVERVSL
                     TTAATAESGLEGAAREARYAAFAKLDVDLLLLAHHRDDQAETLLLNLGRGCGLAGAAA
                     MPDERPLGPGNAAIGLRRPLLGVARADLERYAADAGLGWIDDDSNADLDLARNRLRHQ
                     VLPAFEEARPGAGANLAAASRRFSEALSLLDELADLDLAAASSAPDARIEQAPLLALS
                     PLRRINALRRWLTCAAIGIPGEARLIELAEQIAEVGTNSRLRVDFGAEHSVRLWRGRL
                     YLVRERPAVTTEARLPLKDGSAVDWGSGRVRLQATVGAGIAAVKIGDELVLRPRSGGE
                     RLRPAVDRPSRSLQELFAMAGIPPWSRTQLPLLWDGGRLLWVAEVGFAGGCAAAAGEP
                     GWLPLWEPARD"
BASE COUNT         4313 a         7773 c         7491 g         4232 t
ORIGIN      
        1 gtagttgccg agcacctcga cgatgcggcc aatcggcttg ctgtaacgcg tcggcggctc
       61 ggtgatctcg gcaacgacca cctgacctga ctgcgggatc ggctttgcca gcgcgtcggg
      121 cggaatcagg atgtcttgcg acacgcggcg gtcctcgggt acgacgaaaa caacgccgtg
      181 ttcgacaagc acgcggccga cgatcttcgt cgcgccgcgc tcggtgacct cgacgatcac
      241 cccctcgggg cggccacgcc ggtcgaggcc gacgattcgg ccgatgacgc ggtcaccatg
      301 cagcaccttc tccatctgcc gcggcccgag aaacaggtcg tcaccgccgg catcgggaat
      361 caggaagccg tagccatcct tgtggccatg cacgcgcccg gggatcagct gtgccttctc
      421 gggaacgatg aaggcgccgc ggcggttctg cattagctcg gcttcgcgcg ccatggcgcc
      481 caaacggcgc tggaacatta gttcctcgtc ctcgcggatg tcgagcgcct cgcacagttc
      541 gacgaagtcg gccggtcgcc cgagcgcctc gagggtctga cggatgtact cgcgcgaggg
      601 cagcgggaat tcgtagcgcg ccgattcgcg ctccaggaat gggtcggcca ggcgctttct
      661 ggagagctgc tcggtcgatt tcgggttggc ttttttcatt gacatttacc tcgttttctt
      721 tagaatgcgc gcttctttcg cgcctgccca ggtggcggaa ttggtagacg caccaggttc
      781 aggtcctggc ggtggcaaca ctgtggaggt tcgagtcctc tcctgggcac cagcaataca
      841 aagcaaaaag ccggtctttg gaccggcttt ttgtcttttc ggatcaggcg ccgaacgggt
      901 gacgacgaag aatcgtttcg tcgcgttcgg gcccggttga aatcacgtcg atcgggatct
      961 cgcagatttc ttcaatacga tgcaggtagg cgcgtgcttc gggcggcagg tcgtcccagc
     1021 gcttcgcgcc aaaggtcgta ccgccccacc ccttcatcgt ttcatagatc ggtttgcagc
     1081 gggcaacctc gtcggcaccc atcggcagca ggtcgatctt ctcgccgtcg agttcgtagc
     1141 ctaggccgag cttcaactcg ggcaggccat caagcacgtc gagcttggtg atgcacagcc
     1201 cggtgacgcc gttgatgatg atcgagcgct taagcgccgc gagatcgagc cagccgcagc
     1261 gacgcttgcg tccggtgact gtgccgaatt cgcggccgac gcgcgacatc acctcgcccg
     1321 gcgtgccggc agtctcaata tcaagttcgc tcgggaaggg cccgccgcca acccgcgtgc
     1381 agtaggcctt ggtaataccg aggatgtagt gcaaccgggt cggcccgacg ccggagcccg
     1441 cagcggcctg gccggcaacg cagttgctcg aggtcacgta gggataggtg ccgtggtcga
     1501 tgtcaagcag cgtgccctgc gcaccttcga acagcaggtt gtggcctgcc ttgttggcgg
     1561 cgtagagcgc cgccgagaca tcctcgacca tcggcttgat cgcctcggca tcggccattg
     1621 cctgatcgta gaccttctgg aattcgaccg gctcggcctt gaggaacttg gtcagtacga
     1681 agttgtgata gtcgagcagt tcacgcagct tggtcgcgaa gcgctccttg tcgaacaggt
     1741 catagacgcg cagggcgcgg cgcgcaacct tgtcctcata ggtcgggcca atgcccttgc
     1801 cggtggtgcc gatcttgtcg ccagccgact tggcggcctc gcgggcgcgg tcaagcgcga
     1861 cgtggtggcc gagaatgatc gggcatcccg gactgatgcg cagccgctcg ctgaccttta
     1921 gcccaccggc ttcgaggttg cggatctctt cgagcaggtg gtgaatgtcg agaacgacgc
     1981 cgttgccgat gtagcagcgc acgccttcgc gaacgattcc cgaaggtacg aggttgagct
     2041 tgtactcacg ggtgccgatg accagcgtgt ggccggcatt gtgaccgccc tggaagcgca
     2101 ccacaccttg ggcgtgatcg gtcagccagt cgacgatctt gcccttgccc tcatctcccc
     2161 attgcgtacc gacaactaca acgttcttgg ccatttcccg cgccccttgt tttcgctcag
     2221 tgagcgttag ctatcatcca gtcgccgttg cggcagacca gttcgcggtc acagcgcggc
     2281 agttccggct gcgcctcccc aggcaaacat tgcacgacaa tctcgccggc tgcacgcagc
     2341 tcggcgattc gcgccagcaa ccgcgcgtcg ctgccggccg gcgcgagcac cgcgctcgcg
     2401 gcggtgtcgc tcacggtcag ccgggccagt tcacgcaggt cgaggctgaa gccggttgcc
     2461 ggtcgcgccc gcccgaaggc tgcgccaacg ttgtcgtaac ggcctccgag ggcgatcgga
     2521 ccagcaaaac ccgcgcagta ggcggcaaac accacgccat tgtggtagtg ataaccgcgc
     2581 aggtcggcca ggtcaaaact cggcgcgcgt ccggcaacgc cagcggccag tcggtcgagc
     2641 acatcgagcg cctcggcgat ggccggcgaa tctggcaggg ccgcgcgcgc gtgcgccaag
     2701 accggtggcc cgccgaaaag ctcgggcaac ctgagcagcg ccgaacgcca gggatcgccg
     2761 cacttggcca gcgcctcggc aagtgccggc aggtccttgt tctgcatgat cgcgaacagc
     2821 gcttcttcct cggcgccggc gatgccggcc gcgctggtca gcgcgcggaa gatgccgacg
     2881 tgaccgatat cgagccggaa gtcggccagt ccgccacatt cgagcgttgc cgccatcagc
     2941 cgaacgacct caatatcagc ttcgcaaccc gggtaaccgt agagctcggc gcccagctga
     3001 atgggctcgc gcgacgcggt tgccgaggcc ggcagcgcgt ggaggacgct gtcgcagtag
     3061 cagagccggg taacgccagc gtgattgagc aggtgcgcgt cgatgcgggt gatctgcggc
     3121 gtcatgtcgg cgcgcacgcc catcgtccgc cccgacagct ggtctgtcag ttcgaatgta
     3181 cgcaggcgca gatcgcgccc ggcgccggtg aacagcgact cgaggtattc gagcagcggc
     3241 ggcatcacca gcaggtagcc gtgcgtgcgt agctggtcga ggcaggccct gcggacgcgt
     3301 tcgatgtgcg ccgcctcacg cggcagcgca tcggcaatgt attcgggaag aagccagttc
     3361 acaggaacac cagaagcatg aacagcccga cgatcatcga ggtcaggcca atgaaccgca
     3421 cctggccgtc actcatcgcc accagacgtt tgaaggtttc gcgccaggtg cccggggcga
     3481 cgaagggcag aacgccctca atcaccagca tcagcgccaa ggcggcgagc agattgtcga
     3541 tcacggcgcc ctgccccccg cactggcctt acttgccgct cttcttgagg tacttgaaga
     3601 agtccgagct gggatcaacg accagtacgt cgctcttgtt gcggaacgac tctcggtaag
     3661 cctcgaggct gcgataaaag tcgtagaact ctgggttctg cccgaaggcg cgcgcgtaga
     3721 tcgcggttgc cctggcatcg ccgtcaccct tgatctgctg cgactcgcgg taggcattgg
     3781 cgatgatcac ctcgcgctga cgatcggcat cggcgcggat cttttccgac tcggcgaaac
     3841 cggtcgaacg caactcgttg gcgacgcgct tgcgctcggc ctccatccgt cggtagaccg
     3901 aaacgctgac ctcgtcgggc agctcgacgc gcttcagacg gacatcgacg atctgcacgc
     3961 cgatcttgcg ggcgtcgagg tcggccttga tccgcatctc gtccatgatc ttgtcacgct
     4021 cgcccgatac cacgtcgtta accgtgcgct tgccgaactc ctcgcgcagg ccggcattga
     4081 ccgtctgaga caggcgcgtg cgggcgcgcg tttcgtcacc ctgaaccgag atgtagtaga
     4141 gccgcgggtc gatgattcgc cacttgacga aggaatcgac aagcacgttc ttcttctccg
     4201 atgtgatgaa acggtccggt tcgggcgaat cgagcgtcag gatgcgcttc tcgaagaaac
     4261 gcacgttctg cacgaaggga aacttgaaat gcagacccgg cttgtcgatg acggtcttga
     4321 cctcgccaag ctggaaaacg atcgcgaact gacgctgatc gacggtaaac agcgtcaccg
     4381 aggccagcag gatgccggcg actagaaagg cgagcacgaa tttcagacga ttggccatgt
     4441 cagcgaccct cccgattgcg tgagcgcagc gaatcgcgtc gcggcggcgc cggatcggcg
     4501 acggccggtt cctggacctt ggcggcagct gccgccgcgc tttcagcggc ggcaatggcg
     4561 ccggccgagc gcatcagctg atcgagcggc aagtacaaca ggttccccga ccccttggtg
     4621 tcgagcatga tcttgccggt gttggcaaag acctcctgca tcgtttcgag atacatgcgc
     4681 ttgcgtgtta cctcgggtgc cttcgagtac tcggtgtaga tctggcggaa gcggctggca
     4741 tcaccctcgg ccgtcgcgac aacccgttgc ttgtaggcgc tcgcctcctc aagcaggcgc
     4801 gccgcgctac cacgggcctt cggaatcacg tcgttggcgt agccctcgcc ctcgttcttc
     4861 tggcgctcgc ggtcctgccc ggccttgacc gcgtcgtcga aggcggcctg tacctgctcc
     4921 ggcggctgcg cgttctgcat ggtgaacttg gagacgatga tcccggtctt gtagcggtcg
     4981 agaatgtcct gcatcagcac ggcagcctgc tgtgcgacct gctcgcgacc ttcgtagagc
     5041 acgtagtcca tgttgctctt gccgacgatt tcacggatcg ccgtctcggc cgcctgcgtg
     5101 actgcctcgt ccgggttccg gttgttgatc acgtattcga gcggatcctt gaggaaccac
     5161 tgcaccgcga actggatgtt gatgatgttc tcgtcctgcg taagcatcag cgcttcggcg
     5221 agcaccttgt tcttctcgct tccgcggtaa ccgatctcgg cggtacgcac gccgctgagg
     5281 ttcaccagtt catggctttc gatgggaaac ggcagacgcc agcgcagacc cggatcggtc
     5341 acctcggtga ccttgccgaa gcgcagtacg accgcccgct ggctttcgtc gacgatgtag
     5401 aagccgctcg ccgcccaggc cgccgcgatc aggaacagca cgatgccgac gccgccaccg
     5461 aaaagcttgg gatcgatgtc ggcgccgcga ttgccgccac cgccactgct gccgccaccg
     5521 ccaccacgct tgccgaggat gccggccagc cggcgattca ggtcgcccca gagctcttcc
     5581 agatcgggcg gtccctgatt cggacgacgc ccaccgttac cacgatttcc ccattcggga
     5641 tcattcatcg acatgagcaa ccctatgcta gacatcttca aagaccatca acgtgcgcaa
     5701 cgtccggctc gccggacggc aattcttcat cagactggga cgtggattgc gcgaattccc
     5761 gccgcttttc cttcgcgcgc tctgcaagcg cgtctctcag cagatcgagc ccggcaccgt
     5821 tcagcgcact gagccgaacg cgcgagattc tatcataggc gtccacttca acccccggcg
     5881 gaagcgccgt cagatcgcac ttgttcaaga cgacgacctg cggcacatcg tcggcgccga
     5941 tttctgcgag aaccccgtcg acggcggcaa tctggtcatc acgatcggga gccgacgagt
     6001 cgacgacatg gatcagtaga tcagcctgcg ccgtcgcctc cagcgttgcc ttgaatgcct
     6061 cgaccaatgc atgcggcagc tcgcggatga aaccgaccgt gtccgagacc accgcatgca
     6121 gtcccggttc gagaaagacc ttgcgcgaag tcgtatcgag cgtggcgaac agctggttgg
     6181 cggcaaagac gccagccttc gtcattgcat tgaacagcgt cgacttgccg gcgttagtgt
     6241 agccaaccaa ggagacgttg agcaccgatc cgcgggcccg cgcgcgacgt tgggtcccgc
     6301 gttgccggtc aagccgcgcc aggcgctgct tgagtatctt gacgcggttg ccgagcaggc
     6361 ggcggtcggt ctcgagctgc ttttcgcccg gaccgcgcag gccgatgccg ccgcgctgcc
     6421 gctcaaggtg ggtccagcca cgcacgagcc gcgtcgccag atgctccagt tgcgccagtt
     6481 cgacctgcat cttgccctcg gcgctcgacg cgcgcagcgc aaatatgtcg aggatcaggc
     6541 tggtccgatc gacgacgcga cagttgagcg cccgttccag gttgcgctgc tgggccggcg
     6601 aaagcatgtg gttgaacacc accagttcgg ctccggcgcc agccacctgc gcggccagtt
     6661 cggcgacctt gcccttgccg gcaaaggtcg ccgcatctgg tgcagctcgc ttgccgagca
     6721 tttcgccgac gatgcggcca ccggccgatt gcgtcagcag gcggatttcg gacagttgtt
     6781 cggcgcgctc atagtcctcg acgcggccga tatcgaactg gacgacgacg acgttttcgc
     6841 cggaggccgg ccgttcaaac acgtggcgcg ttgcctccgg aaatcagtct gcgcccgctt
     6901 cctgctggat ggtcacaggg cgagcgggga cgacggtcga aatcgcatgt ttgtagacca
     6961 tctgggtaac cgtgttcttc aacagcacca cgtactggtc gaaggattcg acctgaccct
     7021 gcagcttgat gccgttaacg aggtaaatcg agacggggat gtgttctctg cgaagcgtat
     7081 tgaggaaagg atcttgtagc agttgtccct tgttgctcat ggtgcgtact ccatatcgtc
     7141 gttgttatgt ggatgcagcg tctaatgtac ttgactatcg ggtacccgga aagaacgcct
     7201 accccggctc tgacccaacg accggccgtg agttcgcgac gaatccgcct atcgccgcgg
     7261 tttctcggcg taggggttct tcgccacatt aaactggatg cgcagcggcg tgccctgaag
     7321 cttgaaggcg tcgcggaaaa cgtgctccag atagcgccgg tagctgtccg gaacatgatc
     7381 gagcgcggtg ccgtgaatca cgatgatcgg cggattgttg ccgccctggt gggcatagcg
     7441 catcttgggc cggaaaatcc cgtgacgcgg tggcgcctgc cgcgccacgg cctcggtcag
     7501 cgttcgcgtc aggcggggcg tcgccatctt ggccatcgcc gccgcgtagg cgccatcgac
     7561 cgagcgcatc agcgcagtca gcccctgccc ctcgagcgcc gacacgaaat ggtgctttgc
     7621 aaaatcgagg aactgcaggc gacgggcgat ctccgccttg atctgcgtgc gccggtagtc
     7681 atcgatgcca tcccacttgt tcacggcaac gacgagcgcc cgccccgact cgacaacgaa
     7741 tccggcgatg tgcgcgtcct gatccgaaat gtcctgctgc gcgtcgagca cgaggacgac
     7801 gacgttggcc tgctcgatcg actgcagcgt ctttaccact gagaacttct cgatcgcctc
     7861 gaagaccttg ccgcgccgcc gcaatcccgc cgtgtcgatc agcgtgtatt ggcgctcgcc
     7921 gcgcgtgaag gggatctcga tcgcgtcgcg cgtggtcccc ggcatgtcga aggcgatcac
     7981 gcgctcctcg ccgagcaatg cgttgatcat cgtcgacttg ccgacattgg gacggccgac
     8041 gatcgcgatc cgcggtgatt cgttggccgg ctcgccggtg ctgtccggcc attcggcgag
     8101 cgcatcctcg atcaggtgac gaacgccctc gccgtgtgcg gccgagatcg gctgtggctc
     8161 gccgaggccg agcgagtgaa agtcggcggc ggccacgccg gggtcgaatc cctcggcctt
     8221 gttgaccgca acgaccaccg gcacgtcgag ccggcgcagc ttattggcga tttccttgtc
     8281 gagcggcgca acgccctggc ggccatcgac gagaaaaacc acgagatcgg cttcggcaat
     8341 cgcctgttcg gtctgccgcg ccatctcgta aacgatgcca tccttcgcta gcggctcgaa
     8401 gccaccggtg tcgaccacca ggaatggctt gctgccgatt cgcccgtgtc cgtagtgccg
     8461 gtcacgtgta aggcccggca ggtcagcgac cagtgcgttg cgactctttg tcagccggtt
     8521 gaaaagggtc gatttgccaa cgttggcacg ccccaccaac accacggtag gaatcatctt
     8581 cagtccagtt cgactgcgag aacgccgccg cggcgggtct gatagatcgt cgtgtcaccg
     8641 gcaatcaccg ggtcgctgct gaaacccccg ccatcgctgg cgagacggcc aaccagtgcc
     8701 ccatcgctga ggcgcagcgc gtgcagtacg ccgctgccat cgccgacgag caaaaaaccg
     8761 cgacgtacca gcggtgcgcc gagccggcgc cacttgagtg cgtcctgctg ccagaccagc
     8821 ttgccgtcgg cacgggcgta ggcatggacg actccgtcgg tgtcggcgac atagatgttg
     8881 ctgctgtcga gcgcaatgct ggaatcggcc gaaacctcct gcgcccagat cagattgccg
     8941 ctgctcaggt cgaagcaggc aacgcggccc tgataggccg ccgcgcacac ctcgcgcgga
     9001 ccgagaaccg gcgaaccggc gatgtcggcg atccgctcga gttcggtggt gcccttcgac
     9061 agcgcaaccg atccttccca gagtaccgcc ccgttggcaa cgccaaccgc gagcagtttg
     9121 ccgccgggat agccaaggaa ggcgaagttc tcggcgaaaa ccggcggcgt cgtcacgcgc
     9181 agcgtcagcg ccggcgtcgt ccgctgcatg acccattgcc gagtgccatc gcgtgcgtcg
     9241 agcgcaaaca ggcgatggtc gccgctgcga accagaatgc gatcgccggc aaacaccggc
     9301 ggcgccaaga cttcggaact gacacgggca cgccagcgtt cactgccatc gttcgttgcg
     9361 aaagcgacta cttcgccctt gctggtgccg accacgacaa tctcgccgtc ggtgctcacc
     9421 ccgccactga tcggcagttt ggtcgcgatt tcccaaaccg gttccccggc tcggtagcgt
     9481 ccgagccggc cgtcgggtgc ggcgatgaac acggcgtcgg cattcgccgc cgggcgcaac
     9541 gcggcatgac cgaggtcgcc gccgggcacc tgccagacaa cgcgaatcga ggtctccgcg
     9601 gtgatctcgg gcagctccag cggcttgcgc gacggttcag acgcgcaacc gctcgccagc
     9661 aacgcgacgg ctaccgccaa tgctgggaac tgccggacag cgagccggtt catttcacgg
     9721 cggccagtgc gtcacgtttt tgcttgagca actcacggta gcgctgggcc gacgcatccg
     9781 tttcggtcgg cattgcggca agcgcagctt cataggccgt ccgcgcttcg gcgcgcttgc
     9841 cctgcaccag caggatgtcg ccgcggactt cggcgaaacg tggcgcgaaa ctcgcctgat
     9901 cgggcttcgc cactgttgcg agcgccgcat cgacattgcc gtcctcaagc aacaccgccg
     9961 tcagacgcag ccgcgcgaga tcgcgcaatt cgctgtcagc atggtcgacc acccactgca
    10021 gctgggcctg cgccgtcttg cggtcgcccg cctcaaaggc gacgcgcgcg gaaatcatcg
    10081 cgcccatctc ggcgtagcgt gtcgaagagt atttctcgag cagttcgccg gccgctgcgc
    10141 gaacccgctg gtcttcctcg agatttgccg cctgctgtag cgaggcaaat agcgccgagg
    10201 cttcggcgct ctgattgcgc tgataccagt tccatccctg atagccggcg aaactcagcg
    10261 agacgacgag cagcacgccg gttatccagt cgccgtactg gcgccaccag gccttcaggc
    10321 tgtcgagttg ctcctgctct tccagatcgt acgtcgccat ctcagttttc ctcccaatcg
    10381 atcatttcat cagtcaattc atcggcaagc gtcgccaccg ccacgcgccg ctgcggcccg
    10441 ccggtgcctc cctcggtgcc gtcggcacgc agggccttga gcgtcacttc gtcggccgcc
    10501 gcctcatcgt cgccgatgat caccgcgtag ctggcgccgg aaccatccgc ctttttcatc
    10561 tgcgacttga aactgccgcc gccggcatgc atgagcacat cgaagccgac attgcgcagg
    10621 ctctccgcag cgcgatacgc agaacgcctc gccgcctcgc cctgatgcac gacgtagaca
    10681 tcgggaaccg gcgcatccgg tgcaccgccg gcgtcttgga tcaacgcaat cagccgctcg
    10741 acacccattg cgaacccgca ggccggggcc ggcttgccac cgagctgttc gatcaggcca
    10801 tcgtaacgac cgccggcaca gacggtcccc tgcgcaccga ggcggtcggt gacccactcg
    10861 aacacggtca ggttgtagta atccagcccg cgaaccagcc gcggattgat ttcgtagggc
    10921 aggccctgat ccttcaggac ctgctgcacg gcctcgaagt gcgcgagcga ctcgggcccg
    10981 agataatcga tcaggcgcgg cgcgccggca atcatttcct gcatctccgg attcttgctg
    11041 tcgaggatgc gcagcgggtt gctgtgcagg cgccgcgtcg cctcggcatc gaggcgatcg
    11101 gtgttcgact cgaggtaggc gatcaagtcg ctgcggtgcc gggcgcgctc gtcggcctgg
    11161 ccgagcgagt tgatctgcag gcggatgcca tctagcccga gatcgtccca gaggcgcgcg
    11221 cccatcagga tcatctcggc gtcaatatcg gggccggcca gccccagcgc ctcgacgcca
    11281 atctggtaga actggcggta gcggcccttt tgcggtcgct cgtggcgaaa catcggtccc
    11341 gtgtagtaaa gccgctgcgg gccgcggccg agcagaccgt gctggatcac cgcgcgaacg
    11401 cagccggcgg tgccctcggg gcgcagcgtc agcttctcgg cgttgagcga atcctcgaag
    11461 gagtacatct ccttctcgac gatgtcggtc accgctccga tcgcccgcgt gaagagcggc
    11521 gtcggctcga cgatcggcag gcggatcggc cgatagccgt aggcgcgcag ccagtcgcgg
    11581 acgacctcat cgaagcgctc ccagagatcg gcctcgtcgg gcaagatgtc gttcatgccc
    11641 cggaccgatt gcagctgctg cgtcatgcac caccctttcg cacgtatttc gtcgttacat
    11701 agcgctcgat cagcgcctta aattcttcgg taatccgctc gccgcgaagc gtcacggtct
    11761 tttcgccgtc gacaaagatc ggcgccgccg gcgcctcgcc ggtacccggc aggctgatgc
    11821 cgatgttggc gtgcttgctc tcgcccgggc cattgaccac gcaacccatc acagccagcg
    11881 tcatgtgctc gacgccgtca tactcgctgc gccaagccgg cattcgcgca cggacgaagt
    11941 cctgaacctc tgatgcgagt tcctgaaaat aggtgctcgt cgtacgtccg cacccgggac
    12001 acgcggtcac cagtggcgtg aaggcgcgca gacccatggt ctgcagcagt tcctgcgcga
    12061 cccgcacttc gccggtacgc ggttcgccgg gctccggcgt cagcgagacc cgaatcgtgt
    12121 cgccgattcc ttcctgcagc agcacgccca tcgaagcgca ggaggcgacg atccccttgc
    12181 tgccgatgcc ggcctcggtc agaccaagat gcagcggcag gtcggtgcgt tcggcgagca
    12241 tgcggtagat cgcgatcagg tcctgcacgc tcgacacctt gcaggaaagc acgatgcgct
    12301 ctgccggcag gccgatctca acggcccgcg ccgccgattc gattgccgag gacaccattg
    12361 cttcgcgcat cacggcgcca gcgtcggccg gctcggggcg cctggcgttc tcgtccatca
    12421 cgcgcgccag cacaccctgg tcgaggctgc cccagttcac gccgatgcgg accggcttgt
    12481 cgagacgaca ggcgatttcg atcatgcgtg cgaactgctc gtcgcggcgc gcaccgaaac
    12541 cgacgttgcc cggattgatg cggtacttgg cgagcgtgct tgcgcaatcg gggaaatcgg
    12601 cgagcaggcg gtggccgttg taatggaaat cgccgatcag tggcacctcg cagccgagac
    12661 gcgacatctg ctcttggatg cgcggcacgg cggcggccgc ttcgggtgtg ttgacggtga
    12721 tgcgaacgag ttcggaaccg gcctgcgcca gttcgacgac ctgccgggcc gtgccaatga
    12781 catcggccgt gtcggtgttg gtcatcgact gcacgaccac cggattgccg ccaccgatca
    12841 gcaccgaccc gacccgcacc gcgcgggtca cccgccgcgc cagcatgttc attccaccgt
    12901 cagcttggcg acgttggcgc gtgccctcgc tgcgaggtcg accgcctcgc cgtcgacgct
    12961 cagatcgaca ccgctggcat tgccgacgac gacttcgagc ggcgcaacac cacgcacgtt
    13021 gcgggaagtt ccgggttcga agttgtaggc gatcagggta ttgccgccag cgtcgtggac
    13081 ctcgacccac gacagctcgc gaaccgtaaa gcgcaacgcg cgctgggggc cgtcaacgac
    13141 gacaaaggga cgcggcgcaa ccggtgcagc cgcggcctcg gccggtttcg ccggctggct
    13201 tgccgcgggt gctagcggtt ccggcgcccg caccgacggc gcgggcttct cggcgacgac
    13261 cggtaccgcg gtggcagccg gattggcggc cggaactgac ggtggctcga ccactaccgc
    13321 gggcgcagtc gcaaccgggt cggcaactgc agcggccggc gcagccgggc ccggcgagac
    13381 catcggaggg gctggtaccg actccgcggt aggcggcgcc agcggccgct tgggcggcac
    13441 gacgttcggc gccgattcaa tcggccagaa gaaataggca gcaattgccg cgaccagcaa
    13501 caagccgcca ccggccagca tcggcatgag ctgcgaagac gacgagccct cagcgggcat
    13561 ttcggcgtgc gagccttccg gcaactcgat cggctgcggc gttggggcgc tgaccgaggc
    13621 cagcaagggc tcgggatcga ccttgagcag ccgcgcgtaa ttgcgcagga agccgcggac
    13681 gaaggtttcg ccgggcaact gatcccaagc atcggactcc agcagaccga tctgccgcgg
    13741 actcaagcgg atcgattgcg ccgcgtcagc aatgctgatt ccagctacct cgcgcgcggc
    13801 ccgcagttgg gcacccggcg acggcgacgg cgacggcgac ggcgacggcg acggcgacgg
    13861 cgacatttcg acttcgatga ccttgtcggt tcccggcaac tcggactgcc tgttctcgct
    13921 catccgccaa ccccctgccg cgattcacgc gcctctttcg aatcgggaaa acggcgccgc
    13981 agctggctga tgaagccggc ctcggccctc cggtcgccgg ccgcgcgctc gacacggatg
    14041 gccagccaga gcgattcggc ggtcggctcg acctgagcgt gcagcttggc gaggtagtcg
    14101 cgggcatcga cgtagcgctt ctggcggtag ttgagatgcc ccagctgcaa cagcgccccc
    14161 ccggtattgc ggccaagccg caaggccttg atcaggtaat cctcggctgc cgccagatcg
    14221 ccgagctgca ttgcgcagat cccggcgttg gtcagcgaca gctcgggggt tgcgtacaac
    14281 gggttcttga cggcgctgac gaactgggcg atcccgtcgg cttcacgacc ggtctgacag
    14341 aggaaccacc cgtagttgtt gctgatttcg ggatcgccgg gcgccagccg cagaccttcg
    14401 cggaaattct cttcggcaac cggattctcg cgcaggtaca tgtgaatcag gccgctcagg
    14461 ttgtaggccg gcgcgtagct gcggtcggca ctgagcgcaa ttcgcacttc ctctagcgcc
    14521 accgccatat tgccggcctg gaaatacgcg gttccgagtt cgatgtgggc gcgcgcccgc
    14581 tgtgtcttgg cgctcgacag tgctgcctgc gaggacggtt cgacggcacg ttcggccgtc
    14641 gacatgcccg acccggcgca gcctgcaagc agcagaccga cacacaacaa cgggatcatc
    14701 gctaggcgac ggactggatt catcggtgga actccacggt tctcgacgtg cggcgggtcc
    14761 ggtcgagtac ttgcccggca agctggccgc aggccgcgtc gatatcgtcg ccgcgggtct
    14821 tgcgcgtcgt cgtgacgatg ccggcctgct ggatcagcgc agcaaaagcc ttcacccggg
    14881 ccggcgacgg acgtcggaac ggtgagccag ggaagggatt gaaaggaatg aggttgtact
    14941 tgcacggcac ttcgccggca atctcgacga gcttgcgcgc atgcgcatcg gaatcgttga
    15001 cgccttcgag cagaacgtac tcgaaggtga tgaagtcgcg aggcgccgca ttcaggtaac
    15061 gccgacacgc cgccaacagc tcagccagcg gatagcgccg gttgatcggc acgagttggt
    15121 cgcgcaaggc atcgtcgggc gcatgcagcg acacggccaa cgccaccggg caggcctcgg
    15181 caagccgatc gatcgccggc accagtcctg ccgtcgagac ggttacgcgg cgacgcgaca
    15241 gaccatacgc gtggtcgtca agcatcaggc gcagagctga cacggttgcg tcgaaattcg
    15301 ccagcggttc gcccatgccc atcatcacga cattggagac cggccgctcg ccgcccgacc
    15361 cacgaccgat cagcgcgttg gcctgccaga gctggccgat gatctcggcc gtcgtcaggt
    15421 tacggttgaa gccctgcttg ccggtcgagc agaacgcgca gtcgagtgcg caaccggcct
    15481 gcgtcgacac gcagagcgtt ccgcgatcgt cttcgggaat gaacaccgat tcgatggcat
    15541 tgcccgagcc gacgtcgaaa aggaatttgc gcgtgccgtc atccgacagc cgatcgctga
    15601 tcagcggcgg cggcgccacg acggcgctct ccgccagcca tgcgcggagg ctgcgcgcca
    15661 gatcggtcat cgccgcgaaa tcgctggctc cggcctgatg gacccagcgc agcacctgcg
    15721 tcgcacgaaa ggccttctcg ccgcgggcaa gaaagaatgc cttaagcgat tcgtgatcga
    15781 aatcgaggag attggcggct tggtcgttca cggctttggc gtcgattaca cagcgatcgc
    15841 gccgaccgcg atcggatgac cgggtcggcg gcgcgggtgc tcagcggcta tagacgttca
    15901 tttcggggaa gaaaaaggca atctcgacgg ccgcggtctc gggtgcgtcc gacccgtgaa
    15961 cagcattggc atcgatcgag tcggcgaagt cggcgcggat cgtacctgca gcggccttct
    16021 tcggatcggt cgcgcccatc aggtcgcgat tcttagcaat tgcgccctcg ccctcgagaa
    16081 cctggatcat caccgggccc gaggtcatga acttgacgag gtcgttgtag aacggacgct
    16141 cgcgatggac ccagtagaac ttgccggctt cgatcgtcga caggtgcgcc atcttggcgg
    16201 cgatgacctt taggccgttg gtctcgaaac gggaataaat ctttccgatc acgttcttgg
    16261 caaccgcatc gggcttgatg atcgacagcg tacgttccac agccatggtg caaaactcca
    16321 attagacaaa gggttaggaa aaatcgagaa cgaggattct agcaggtttg ccgttcgccc
    16381 cggcgcttct tcgggtcttt tcggcaatcc cgaaaggaaa aacgccggcg tttccgccgg
    16441 cgtccctgga aagacgagtt cgaaggttca gaactacatc atccccaacg tcttcggcag
    16501 ccacagcgag atcgccggta cataggtgat caggatcagg aagaccagca tcgtatacag
    16561 ccacggcagg accgccaggg tcatctcggt catgccggcc ttggtaatgc cgctagccac
    16621 gaacaggtta aggccgaccg gcggcgtgat cattccgatc tccatgttca cggtgatgat
    16681 gatgccgaag tgcacagggt cgatgccgag cgcggtcgcc accgggaaca ggataggcgc
    16741 caggatcagg atgatcgacg acggttccat gatgttgccg gcaagcagca gcaggacgtt
    16801 caccaccacc aggaagccga ccggaccgag gccgctggcg acgatcgcct cggccatccg
    16861 ctgcgggatc tgctcggaag tcaggatgaa cgagaagagc acggcgctgg caatgatgaa
    16921 cagcagcatc gaactcatgt tcgccgcatc gagcagcaca cgcggcacct ggcgaaagcc
    16981 gatgtccttg tagacaaaca tcgcgataaa gaacgcatag accgcactca tcgccgccgc
    17041 ttcggtcggc gtaaacatgc ccgaatagat gccacccatg accacgacga tcagcatcag
    17101 gccccagacc gcttcgcgga aataggtcca acgctccttc caggaggcct tgggctgacg
    17161 cgggtagttg tgcttcttcg cctgccacca ggtcgtgaag ccgagcatga acgccagcat
    17221 gatcccggga atcacgccgg ccatgaacag cgcgccgatc gacgaatcgg tcgacaccgc
    17281 gtacatgacc atgacgatag acggcggaat caggatgcca agaccaccgg ccgaggcgac
    17341 gacgccagcc gagaagcgca gcgggaagcc ctgcttgagc atcgccggaa tcaggatcga
    17401 accgatcgca accacggtcg ccggcgagga acccgagacc gccgcgaaga tggcgcaggc
    17461 cagcaccgcc gacatgccga gaccgccggt catgtgaccg accatcgccg tcgcgaagtt
    17521 gatcattcgc cgcgcgacgc caccgtgggt caggaagttg ccggcgagga tgaagaaggg
    17581 aatcgccatg atctcgaact tctcgatgcc ggtgaacatc ttcagcgcca ccgcttcgat
    17641 tggtgtcgag gtaaaggccc acatgaaggt cagcacggtc aggcccaggg ccacaccgac
    17701 cggcatgccg accgccatca gcgtaatcag gagaccgaaa atcaccaggg tcgtcatgat
    17761 ttatttctcc tgttttccgt tgttcttggg atgggtcttt tcccactcgg cgtcgctctt
    17821 gccgtggtgc gcgtgctcgc gctcctcgaa ggcctcgatc gcctcgccga gcatgtcggg
    17881 cgccggctcc tcgatgccgt caacgtgtcc gtggtcgtga tgcggcagtt cgccggtctt
    17941 atggaaacgg atcattgcct gaatgaagcg gaagcacatc aacccggaac cgagcggaat
    18001 cgcggcgtat acacgccagg tcggccactc gaggtcgggg gtgatcggcc cttcgaagta
    18061 gtcgcccgcg tcgcgaccca gcgcggtcag cgtttcgtag gccatgccgt tttcccagac
    18121 gaagacggca ccgaaaacgg cgatgacgcc ggtaaaaaag acgccggcga agaggccaat
    18181 ctggatcagg acttcctgcg gcttgccttc gagcttgttg atcagcagat cgacaccgac
    18241 gtggatgccg gtgcgcacgc cgtaggccgc gccaaacttg gccatccaga caaacaggat
    18301 caggcagagc tcctgcgccc aacccatgtg aatcgacagc aaccagtcct gcacgcccgg
    18361 aatcgcgaat ccggaaccgt aacggtgcac gacggagatg aagataatga tcgtcgccag
    18421 cgccatcagg ctgccgatga tgaattcctc tagccggtct atgaccttgt tcattgaagg
    18481 cctcctaagc gagataaacg cccgccgggc gctactcgcc ggcgaaaatt gactgcgatt
    18541 aaactgcgtt gcgtgtgtaa gaaagcccgc cgggctatct tcgccggcgg gcctgcttca
    18601 ttgatgctta cagcttgctc gggtcgaaac cggtctcttt gtagaccgct tcgatcagat
    18661 cggcaccgat gcgcgattcc atttccttgt gcaccggaac cagcgccttc ttcatcgcct
    18721 tcttctcgtc ggcggtcagc ttgatgatct cgctcttgcc ggccttggcg acttcggcca
    18781 tggccatttc gttctcttcc ttggcgatgc cgttggcgaa cttggtggcg tcaaccatgg
    18841 ccttttcgag ctgaccgcga atatcagcgg gcagaccgtc ccagaacttc ttgttggtga
    18901 tgaccgcgta gccgaggtag ccgtggttcg actcgacgac gtacttttgc acttcgtgca
    18961 tcttctgcgt gtagaggttc gacggcgggt tctcggtgcc atcgacaacg ccggtctgca
    19021 gcgcctgata gacctcggag aaggccatga cctgcggcag ggcgcctagc gcgcgcatct
    19081 gtgactcaag cactttcgac gactggatgc gcagcttctg acccttgtag tcagccggcg
    19141 tgcggatcgg cttgttcgag ctcatgacct tgaaaccgtt gtcccagaag gccaggccct
    19201 tgataccctt gggctcgagc gaggtgagga atttctggcc gagcggacct tcggtgacct
    19261 tgtgtaggtc tgcgtagccg tcgaacatgt acgggaggtc gaagacctcg aaggtgcgaa
    19321 cgccgagcgg accgaacttg gccagcgacg gggcgatcat ctgcacggcg ccgagctgca
    19381 gcgcttccat ctcttccttg tccttgtaga gcgtgctgtt ggcgtagacc tcgaccttga
    19441 ccttgccgtt ggtgtattgc tcggcgagtt ccttgaactt gatcgcgccc ttgcccttcg
    19501 gcgtgctgtc ggcgacgacg tggctgaact tgacgacgat ttcctgggcg ctggcggtca
    19561 cggcaaagac gccgagcagc atgccgcaga gcaacgtgga gatacgcata agtctcactc
    19621 ctagatttgg tgttgacgat tagccggcga ttgccgagcg gggcggatta tggcgatcgg
    19681 gagccaggct ccggaattgt ggatatccgc aacggcgctg gcgcgctccc gcgccggtca
    19741 tccgcggata tctgtgtaag atcgtttcca tgatgcagcc tgcttccgta gcccaaattc
    19801 cgccgccgcg cgggcgccgc cgcctcaacc tgtacctggc gatgccgaac atcgcgattg
    19861 cgctgctcgt cgttgccgtg atcgggcttc tctgggtgct gcaggagcaa aatcgcgagg
    19921 accggcgagc gacgctgatt gccgacatcc tctggctgga acagaacctg cgcttcgagc
    19981 tggtcggcgt tcgcgaccgc ctttcccagc tcgccaacgc cctgccggcg gaggcaaatc
    20041 cgcgggaatt tttcgatcag catattcgcc agctacgcca aggcaatgcc gagatcgttc
    20101 aggtcatctg gctggacgaa accggtcgcg tctaccgcgc caatccggcg ccaacccatc
    20161 cccacctgat cggcggcgcg atcgccgatc agctactgag cgaccggatc gaggttgcgc
    20221 gtcaactggg gcgtcgcgtc tacaccgccc cgtactcgct cgacgacagc ggagcccagt
    20281 tcgagatcgt cgttccgctg ttcagggacg gccagtacgc tggcgcgctg attggcgtgc
    20341 attcgcttca aagcatgctc gaccactcgg tcccctggtg gtttaccgag aagtaccagc
    20401 tcgaggtaat taacaacgaa ggccagatca tcggcagcaa atccaacatc gtcggcaagc
    20461 cacaaagcac ctataacctg gtcctcgatg aactgggaca gggcgtcacg ctgcgcgctg
    20521 gcctcacccg ctcggacacc gaccgcgcgg cccgcttcgt tgtcgcggca atcctcgtcc
    20581 tcgccgccgc ggtgttctgg agcctgtggg caatccgcgg cctgattcgc cgtcgcctgc
    20641 gtgccgagga gcaattgcgt ggcgccaatg cgtttcgcga atcaatggaa aactctctgc
    20701 tgaccggcct gcgcgcccgc gatctcgatg gccggctgat ctacgccaac ccggccttct
    20761 gccgcatggt cggcttcagc gaaaccgagc tgatggggca cctgccgccg atgccttatt
    20821 gggatccggg atcagttgag gaaaccatgc gaattcacca ggaggtgcta cacgggcatg
    20881 cgccgccaga cggattcgag atgcgttttc gtcgcaagaa cggcgaaggc ttcgacgcgc
    20941 ttgtttacga agcgccgctg atcgatggcc acggcgaaca cgtgggctgg atgggttcgg
    21001 tgctcgacat caccgaacgc aagcgcatcg ccgaattcaa gcggcagcag gaggacaaac
    21061 tgcagcacac ggcccgcttg gtggcgatgg gtgagatggc ctcgacgctg gcccacgagt
    21121 tgaaccagcc gctcgcggca atcgccagct acacggccgg ctgcctcaac aagctcgaga
    21181 gcggtcgcca caccgaggcc gagctgacga ccgtgctgcg caagatcaac gcccaatcgc
    21241 agcgtgccgg caagatcatc cgccaggtcc acgacttcgt ccgccgccgc gagccgcggc
    21301 gcgaagcctg cgcagtcgcc gaggtcgtcg atgaggcggt gactctgttc gaggcacagg
    21361 cgaagaagca cggggtgcgc atcgcctgca gccttcaggg tgggctaccg gaggtgtcgg
    21421 ccgatccgac aatgctcgag caagtagtga tcaacctgct gcgcaatgcc gtcgaggcaa
    21481 tggctgactc cccggccgag gaacgccggg tcgacgtttc ggtccgccgc gagggcgatc
    21541 gaatcgttgt ccgcgtcgcc gacaacggac cgggcatccc tcccggatcg atggaaatgc
    21601 tgttcacgcc cttcttcacc accaagcccg aaggcatggg catgggcatg gcaatctgtc
    21661 gctcgatcat cgaattccac cacggccgcc tcgacgcgcg aaacagcgag gcaggcggcg
    21721 ccgaaatttc atttttcctg cctatcatcc cgcaatgaaa gccctcgccc atatcatcga
    21781 cgacgacgaa gcgatccgtg attcgctcga atggttgctc gaatcacgca acatcccggc
    21841 gcaaagctgg cccagtgccg agtcatttct cgcgcatttc gatgcctgcc gcgggagcac
    21901 gccgggctgc ctgttgctcg acatccgcat ggacggcatg agcggcctgg cgctgtttga
    21961 cctgctctgc gagcgcggcg accgaacccc ggtgattttt ctaaccggcc acggcgacgt
    22021 gccgatggca gtcggcgcgc taaaacgtgg cgccttcgac ttcgtcgaaa agcctttcga
    22081 cgacaatgaa ctggtcgccc ggatcaccga ggcgatggcg atcgacgcgg cgaagcgtgc
    22141 cgcatcggcg gtcagcgcct cggttgccga ctcgctggcg acgctcacgc agcgtgaacg
    22201 tcaggtgatg gaactagtgc tcgccggcaa gctgaacaag atcatcgccg acgagctctc
    22261 gataagcatg cgcaccgtcg aagtccaccg cgcccgcgtt ttcgagaaga tgggcgtcaa
    22321 gtcggcggtt gaactcgcgc aaaaactgat gcgctgaccg aagccggcgc ttaatcgcga
    22381 gctggctccc aaagcggtag ccagccaggc tccccggcgg cggcggcaca gccgccggca
    22441 aagccaactt cggcgaccca cagcaggcga ccgccatccc agagcagcgg cagctgcgtt
    22501 cggctccaag gtggaatgcc ggccatcgcg aacaattcct gcagcgaacg gctcggccga
    22561 tcgacggccg gccgcaatcg ctcgccaccg gagcgcgggc ggagtaccaa ctcatcgcca
    22621 atctttacgg ccgctatccc ggcaccgacc gtcgcctgta ggcgaacgcg accgctaccc
    22681 cagtcgacgg cactgccatc ctttaatggc agacgcgcct cggtcgtcac agcgggacgc
    22741 tcgcgcacga ggtagagtcg cccacgccac agccggaccg aatgctctgc tccaaagtcg
    22801 acgcgcagcc gcgagtttgt cccgacctcc gcgatctgct cggcaagttc tatcagtcgt
    22861 gcttcaccgg gaatgccaat cgcggcgcac gttaaccagc ggcgcaaggc gttgatccgg
    22921 cgcagcggcg acaaagcgag cagcggtgcc tgctcaatcc ttgcgtcagg cgccgatgaa
    22981 gccgccgcca gatcaaggtc ggccagttcg tcgagcaggc tgagtgcttc ggaaaagcgg
    23041 cggctggccg ccgccagatt ggcgcctgct cccggtcgag cctcttcgaa ggccggcaag
    23101 acctgatgcc gcagccggtt gcgcgctagg tcgagatcgg cgtttgagtc atcgtcgatc
    23161 cacccgagtc cggcatcggc ggcgtagcgc tcgaggtctg cccgcgccac accgagcagc
    23221 ggtcgccgca ggccgattgc cgcgttgcca gggccgagcg gacgctcgtc gggcatcgcc
    23281 gcggcaccgg ccagaccaca accacgaccg aggttgagca gcagcgtctc ggcctgatca
    23341 tcgcggtgat gggcgagcag cagcaggtcg acatccagct tcgcgaaagc cgcgtaacgc
    23401 gcctcgcgcg ctgcaccctc caggcccgat tcggcggtcg ctgccgtcgt caaggagacg
    23461 cgttcgacac gcagcggcac accaaggccg gcacagacgc tccggcagtg cgctgcccag
    23521 gcgtcggcct gcggattgat gccgtgatgg acgtggatcg ccgaaagact gcgcgacggt
    23581 tcacccccga cctgcgtcag ggcatgcagc aggaccgtcg aatccagccc gccggaatag
    23641 ccaacggaca ggcggcagcc ttcgggtata tcgcgcaggg ccgcgcgaac cgcagccagg
    23701 acagggcagg cgcccgagcg ggtattaggc gacccgctgc tccttgaacc gaccatagga
    23761 catcagccgc tcgtagcgcg cttcgagcaa ctcgcttggc gacagatcg
//
LOCUS       DATDAA010000073         2537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_144748,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000073 DATDAA010000000
VERSION     DATDAA010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144748"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VIS73_02405"
     CDS             complement(<1..559)
                     /locus_tag="VIS73_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEY5762036.1"
                     /translation="MFEVRIHGRGGQGVVTAAEMLSVAAFLEGRHAQAFPSFGSERMG
                     APVVAYCRISDKEIRLREPVLKPNALIVQDPTLLNQVDVFSGLDRDGYMLINTTRSFD
                     ELGIGDFISDFRPEHLCTVPATELALEHVGRPVPNAALLGGFAAISGRISIDSVCAAI
                     RERFRGRVGEGNARAATEAYQHVLRQ"
     gene            1515..>2537
                     /locus_tag="VIS73_02410"
     CDS             1515..>2537
                     /locus_tag="VIS73_02410"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEY5762037.1"
                     /translation="MTVNKPRSIETTLRSAGLALAAATALTLTACGGGSSSDGAAPPS
                     PPASQPPAPTTGTVGVLFRDGPTDEFCQILASVERIDLLGAAGPTNVFLDSQVIDILA
                     MENYTDIVTVATDVPVGAYEKVRLTLSDLALVECDGEGNPEPESAWEHPKLPGNGKLD
                     LNPRGSFQVVGGETLLIQIDLDMNKSLHLHQTGNGNGKWQFRPVIFVDIIPDGQRLVR
                     VFGQVRDVNGMTFELCPLEPASSVDGGSAPGTGDMSGDDDSGRCLDVFVDGSTGIFNE
                     AGDPASEAGEPVGMDKVVNGGLLTAIGFLSLHDDDDDGDSKADDLRLDAVVLELGDQG
                     TFERIRG"
BASE COUNT          430 a          834 c          857 g          416 t
ORIGIN      
        1 gctggcgcag gacatgttgg taggcttcgg tcgcggccct ggcgttgccc tcgccgacgc
       61 ggccgcggaa tcgttcgcgg atcgcggcgc agaccgagtc gatggagatg cgccccgata
      121 tggcggcgaa gccaccgagc aacgcggcgt tcggcaccgg gcgtccgacg tgctccagcg
      181 cgagctccgt cgccgggacc gtgcacaggt gctcgggcct gaagtcgctg atgaaatcgc
      241 cgatgcccag ttcgtcgaag gaccgggtgg tgttgatcaa catgtagccg tcacggtcga
      301 ggccgctgaa cacgtcgacc tggttgagca gggtggggtc ctggacgatg agcgcgttcg
      361 gcttcagcac gggctcgcgc agccggattt ccttgtcgga gatgcggcag taggcgacca
      421 ccggcgcgcc catgcgttcg gaaccgaaac tcgggaaggc ctgcgcatga cggccttcca
      481 ggaacgcggc gaccgacagc atttccgctg cggtgaccac gccttggccg ccgcggccgt
      541 ggatgcgtac ttcgaacatt gccccctcgc gaagtcaggc cgtccgggcg acggcttggt
      601 ccaggccctc gcagtctgcg aagggccgat ccaacaggca attgtgcgaa accgatagat
      661 gcccggatca cgcttccggg cgcccgcgcc gttgttgccc gggcgcatcc ggtcccagac
      721 ctgcagggcg tgaccgaaat ggcgcgattc ccgaggattt gagggcgaat gggaccagtt
      781 cgcagcctgg ccggtcgcga tggcagccgt cggtgacaac tacctaaccc gcgcggatgg
      841 cccgtctggc gacgatgacg cgacgggagc cggctgattt gcaatgaaat tcatcccatc
      901 tgttgcgtgt aagcaacagg cggcgcccgc ccaacggggc gtcaccgtgt ccaaccattc
      961 cacgacattc gtcgccatcg gcgctccggg ccttgccacg gctgtcacgg ttcccgcggt
     1021 cccggacgga acagggcggc tggctgctgt gcggtgaagc ggccacggtc agccccgggt
     1081 cggacaacct cggttcgacg ccggcggcgt cggtggcggt gtgctcgtcc tggacgtcaa
     1141 ggatgccgcg agcttcggct gccgcgtccc ctgcatgaag accgacaaca tcggcatcga
     1201 aagggagcgg ggtggcgtcg caaggcgtcg cccgtttcat tgcccgtgtc gctcgcgcag
     1261 cagcgaccgc gtgacgatgc ggatgaacgg caggcggctg ccgcggcgac gcgcgccacg
     1321 ctcaacgcga tgaaacgcgg ataccagttg acgaggatca ggccgcatcg cgatttgaca
     1381 tagcgtggcc cgacgcggcg agctttttta catagccaaa tcgctcgcgt tcgtgtcgct
     1441 tcgagacgac atcatcgcct tggtttcacc cgccacatct cctagagtaa ccacacaaga
     1501 tgttggactg gggaatgacg gtgaacaagc cacgatcgat cgaaacgacc ttgcgcagcg
     1561 cgggcctggc ccttgctgcc gcgaccgcgt tgacgctgac cgcttgcggc ggcggctcgt
     1621 catcggatgg cgccgcgcca ccatcgccgc ctgcgtcgca accgccggcg ccgacgaccg
     1681 gtacggtggg tgtcctgttc cgggacgggc cgaccgacga gttctgccag atcctcgcat
     1741 ccgtcgagcg catcgacctg ctcggcgccg ccggccccac gaatgtgttc ctggattcgc
     1801 aggtgatcga catcctggcg atggagaact acacggacat cgtgacggtc gcgaccgatg
     1861 tcccggtcgg cgcctacgag aaagttcgcc tgacgttgtc cgatctcgct ctggtcgagt
     1921 gcgacggcga gggcaatcct gagccggaat ccgcctggga gcatcctaag ctgccgggca
     1981 acggcaagct cgacctgaac ccgcgcggat ccttccaggt ggtcggcggc gagacgctgc
     2041 tgatccagat cgacctggac atgaacaagt cgctgcacct gcaccagacc ggcaacggca
     2101 acggcaagtg gcagttccgg cccgtcatct tcgtcgacat cattccggat ggtcagcgct
     2161 tggtgcgcgt cttcggccag gtgcgcgacg tcaacggcat gaccttcgag ctgtgtccgc
     2221 tcgagcccgc ctcgtccgtg gacggcgggt ccgccccggg caccggcgac atgagcggcg
     2281 acgacgacag cggccgctgc ctcgacgtgt ttgtcgacgg cagcaccggc atcttcaacg
     2341 aggccggcga ccctgcgagc gaagccggcg aacctgtggg catggacaag gtcgtcaacg
     2401 gcgggctgct gaccgccatc ggcttcctga gcctgcacga cgacgatgac gacggcgaca
     2461 gcaaggccga cgacctcagg ctcgacgccg tcgtgctcga gctcggcgac cagggcacgt
     2521 tcgagcgtat tcgcgga
//
LOCUS       DATDAA010000074         6052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_75042,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000074 DATDAA010000000
VERSION     DATDAA010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75042"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VIS73_02415"
     CDS             complement(<1..1049)
                     /locus_tag="VIS73_02415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5762038.1"
                     /translation="MIGREPYVGVTLRVMAIMGVVELAIMFFIEFGLPKHIRHLGVFI
                     DPLLLVVLGAPLVAWWVVRPYERARARAVADANFIAMHDPLTRLPNRRMFSQFLEHAV
                     AGARRRHHYGAILVLDLDHFSEINETFGHEAGDTVLQAVGTHIAGELRGEDFLCRVGG
                     DEFAVALVDLSSERATAERMAREVADKVRFALRRPVPFDDSLLQVDASVGLRLFGGDA
                     VTAEMALREAGAAVVLAKSAGRGQVVLTSDAGAFALATRQTMFAEIERDHLEIDALVD
                     ELILTPTHRLAALQQLADKTLAHLHFEDEVAARVLGDAAEPHRVAHAALAARIETAVA
                     EATENSAPRVGMDIHA"
     gene            complement(1046..1453)
                     /gene="crcB"
                     /locus_tag="VIS73_02420"
     CDS             complement(1046..1453)
                     /gene="crcB"
                     /locus_tag="VIS73_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEY5762039.1"
                     /translation="MHNAFSITNFLAVGIGAACGAWSRWGLGLLLNPLLASLPLGTLV
                     ANLVGGYLIGVAVGFFHWQVGISPAIKLFAITGFLGGLTTFSTFSAEVTERFLAGQAG
                     TALALATLHLVGSLAMTWAGLYTVGAHRIWSAA"
     gene            1690..3495
                     /locus_tag="VIS73_02425"
     CDS             1690..3495
                     /locus_tag="VIS73_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEY5762040.1"
                     /translation="MTRHDQNLPADAPSNFIRNLIEADLASGRYASRRWSGAPGPGAQ
                     QAAGGPDPAKIRTRFPPEPNGYLHFGHAKSILLNFGLAEAYGGRCHLRFDDTNPEKEE
                     QEYVDSIIDAVHWLGCSWQAGDEENRYFASNYFDFMYSAAEALIEAGLAYVDSQSAEE
                     MRANRGTLTEAGKESSFRSRSVAENLDLFRRMKAGEFADGAHILRAKIDMASPNINLR
                     DPAIYRIRHATHHNTGDAWCIYPMYTFAHPIEDALENITHSLCTLEFEDQRPFYDWLL
                     ASLADYGMFARPLPQQIEFSRLNLSYVVLSKRKLIELVEGGHVDGWDDPRLPTLVGAR
                     RRGFTAEGFRLFAERIGVSKADSWIDMSVLEDCMREHLNETAERRVAVLDPVKLVITN
                     YPEGSEELCQAPNHPLHPELGKRDMPFSRELWIEREDFMEEPAKGYHRLYPGNSARLR
                     YAYVVKCTGCERDAAGKVTTVFCEYLPETKSGTPGADNVKVKGNLHWVSAAHGCAAEV
                     RLYDRLFAHPYPGARREGDAEGLERSYLDDINPQSKAVIRAVLEPALRDVAPESRFQF
                     ERHGYFVADRVDSKADAPVFNRTVTLRDSWAKNAK"
     gene            3474..4814
                     /locus_tag="VIS73_02430"
     CDS             3474..4814
                     /locus_tag="VIS73_02430"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEY5762041.1"
                     /translation="MGEEREVEPAAPEAPADAASPPVALPHWRERVDGALQILVTTTR
                     LFLRNELENHAAAVAFYFLLSVVPVVFIVLWLTGTVLQAPALSAHFVELLSGIDQRLS
                     LDELRAAGLLPDAAAGTVGGLSLLAMLWASRQLVLAVQAAFRVINADAQPRAAYLDWM
                     VSLLLVPGAILVIATVSIGRQLIAFLASLAGSGWLADTAPALAGLVSHGLTLTIVWLI
                     VVAAFSGLPPRRPPARVVVRVALLCVLSLVALRVLFATFVSLDRYQGIYSSLGTLIFV
                     LFWVFLAFLVFFFWAQCLYAATHLDAIALEKLMLSAGGERSWLDRHLFGRKSRLTRKF
                     GQSYSAGQVIVREGDESQVAFYLDEGHVGLYRGHSDDTSGERLAAVEAGGTFGEMAYL
                     LKERRSATAIAETDVSVYIIPPTLFEELLALNPRLSRRIIETLAERLRQMNELH"
     gene            complement(4839..>6052)
                     /locus_tag="VIS73_02435"
     CDS             complement(4839..>6052)
                     /locus_tag="VIS73_02435"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY5762042.1"
                     /translation="PSAERSPRPAADTRNAGRFGVEQTAPRPFAWREQKVTAADSPTP
                     AAAPPATSSAAPADAPEPMPASPFSEPPRGAAAPRERRPEKRFDAASESRSERPRESR
                     GEWRAEPRAERRPQPRGEWASETRSERLPSATRAPRPRREEAPPPAAAEDGKSATRLP
                     YGEGIRISKMMAERGLCSRREADEYIELGLVRVDGEIVDQLGARARPDAKITLEKAAE
                     GQQAKRVTILLNKPVGYVSGQPEPGCIPAVTLIKPETQQRSGREREFASGMLRGLAPA
                     GRLDVDSVGLLVLTQDGRIARHLIGEDSQVEKEYLVRVEGTLDETGLKLLNHGLALDG
                     QALKPAKVSWQNEDQLRFVLREGKKRQIRRMCELVGLKVVGLKRVRIGKIRLGKLEVG
                     HWRLLRDDESF"
BASE COUNT          953 a         2107 c         2014 g          978 t
ORIGIN      
        1 gcgtggatat ccataccgac gcgcggcgcc gagttttcgg tggcttcggc gacggcggtt
       61 tcgattcgcg cggccagggc ggcgtgggcg acgcggtgcg gttcggcggc gtcaccgagg
      121 acgcgggccg cgacttcgtc ctcgaaatgc aggtgcgcca gcgtcttgtc ggcgagctgc
      181 tgcagggcgg cgagacgatg ggtgggtgtg aggatcagtt cgtcgacgag cgcgtcgatc
      241 tcgaggtggt cgcgctcgat ctcggcgaac atggtctggc gggtggccag cgcaaaggcg
      301 ccggcatcgc tggtgagcac gacctgaccg cgccccgcag acttggccag gaccactgca
      361 gcgccagctt ccctcagcgc catttcggcg gttaccgcgt cgccaccgaa gaggcgcagg
      421 ccgacgctgg catcgacctg cagcaggctg tcgtcgaagg gcaccggccg gcgcagcgcg
      481 aagcgcacct tgtcggcgac ttcgcgcgcc atccgttcgg cggtggcgcg ctcgctgctg
      541 agatcgacga gtgcgacggc gaattcatct ccgccgacgc ggcagaggaa atcctcgccg
      601 cgcagctcgc ctgcgatatg cgtgccgacg gcctgcagta cggtgtcgcc agcctcgtgg
      661 ccgaaggtct cgttaatctc gctgaagtgg tcgaggtcga gcacgaggat cgcgccgtag
      721 tggtgccggc ggcgcgcgcc ggcgacggcg tgctcgagga actggctgaa catgcggcgg
      781 ttcggcagcc gcgtcagcgg atcgtgcatg gcgatgaagt tggcgtcggc aacggcacgg
      841 gcgcgggcac gttcgtaggg cctgaccacc caccaggcga ccagcggcgc gccaagcacg
      901 accagcagca gcgggtcgat gaatacgccg aggtggcgga tgtgtttcgg caggccgaac
      961 tcgatgaaaa acatgatcgc cagctcgacc acgcccatga tcgccatgac ccgcagcgtg
     1021 actccgacgt agggctcgcg gccgatcacg cggcactcca gatgcggtgc gcgccgacgg
     1081 tgtagaggcc ggcccaggtc atcgccagcg aaccgacgag atgcagggtg gcgagcgcga
     1141 gcgcggtgcc ggcctggccg gcgaggaaac gctcggtgac ttcggcggag aaggtagaga
     1201 aggtagtcag cccaccgagg aaaccggtga tcgcgaacag cttgatcgcc ggcgaaatgc
     1261 cgacctgcca gtgaaaaaag ccgaccgcga cgccgatcag gtagccgccg accaggttgg
     1321 cgaccagcgt tccgagcggc aggctcgcca gcagcgggtt gagcagcagg cccaagcccc
     1381 agcgagacca agcgccgcag gcagcgccga tgccgacggc caggaaattg gtgatcgaaa
     1441 aggcgttatg catcgccgtg acacactccc tgaagctgct tgttctgccc tgcggcgctt
     1501 tatcgatgca gtgtgttcgg cacgcgagca tcccgcctca aagtccgctg gcattctagc
     1561 agccgctgca gcgcagcaga ccgcattccc ctgccgcgga tcgcccagta gaatcgcggc
     1621 ttgcccaatg attgacgggt cggcgcgacg cagccggccc gtcgcttttc taggtttcac
     1681 aaggctcctg tgacccgcca cgaccagaac ctccccgccg acgcgccgag caacttcatt
     1741 cgcaacctga tcgaggccga tctcgctagc ggccgctatg cgtcacgccg ctggtcgggc
     1801 gcgccgggtc cgggcgcgca gcaggcggca ggcggccccg acccggcgaa gatccgcacg
     1861 cgctttccgc ccgagcccaa tggctacctg cacttcggcc acgccaagtc gatcctgctc
     1921 aacttcggcc tcgccgaggc ctacggcggc cgttgtcacc tgcgcttcga cgacaccaac
     1981 ccggaaaagg aggagcagga atacgtcgac tcgatcatcg acgcggtgca ctggctcggc
     2041 tgcagctggc aggcgggcga cgaggagaac cgctacttcg cctcgaacta tttcgacttc
     2101 atgtactccg ccgccgaagc actaatcgag gccgggctgg cctacgtcga ttcgcagtcg
     2161 gccgaagaga tgcgcgccaa ccgggggacg ctgaccgagg ccggcaagga atcgtccttc
     2221 cgcagccgca gcgtcgccga gaacctcgat ctgttccgcc gcatgaaggc cggcgagttc
     2281 gccgacggtg cgcacatcct gcgcgcgaag atcgacatgg cctcgcccaa tatcaacctg
     2341 cgcgatccgg cgatctaccg cattcgtcac gcgacgcacc acaacaccgg cgacgcttgg
     2401 tgcatctacc cgatgtacac ctttgcccac ccgatcgaag acgcgctgga gaacatcact
     2461 cattcactct gcacgctaga gttcgaggat cagcggccct tctacgactg gctgctggcc
     2521 agcctcgccg actacggcat gtttgcgcgt ccgctaccgc agcagatcga gttctcgcga
     2581 ctgaacctga gctatgtcgt gctcagcaaa cgcaagctga tcgagctggt cgagggcggc
     2641 cacgtcgacg gctgggacga cccgcgcctg ccgacgctgg tcggcgcgcg tcgacgcggc
     2701 ttcacggccg agggcttccg gctcttcgcc gagcgcatcg gcgtgtccaa ggccgactcg
     2761 tggatcgaca tgagcgtgct cgaggactgc atgcgcgagc acctcaacga aaccgccgag
     2821 cgccgtgtcg ccgtgctcga cccggtgaag ctggtcatca ccaactatcc cgagggcagc
     2881 gaggaactgt gccaggcgcc taaccacccg ctgcatcccg agctcggcaa gcgcgacatg
     2941 ccgttttcgc gcgagctatg gatcgagcgt gaggacttca tggaggagcc ggccaagggc
     3001 tatcaccggc tctatccggg caacagcgcg cggctccgct acgcctacgt agtcaagtgc
     3061 acgggctgcg agagggatgc tgcgggcaag gtcacgaccg tcttctgcga atacctgccc
     3121 gagaccaagt cgggcacgcc gggagccgac aacgtcaagg tcaagggcaa cctgcactgg
     3181 gtgtcggcgg cccacggctg cgcagccgaa gtccgtctct acgaccggct gttcgcgcat
     3241 ccctaccccg gcgcgcgccg cgaaggcgat gccgaagggc tcgagcgcag ctacctcgac
     3301 gacatcaacc cgcagagcaa ggcggtaatc cgcgccgtac tcgagccggc actgcgcgat
     3361 gtcgcccccg agtcgcgctt ccaattcgaa cggcacggct acttcgtcgc cgaccgcgtc
     3421 gattcaaagg cggacgcgcc ggtcttcaac cggaccgtca ccttgcgcga ctcgtgggcg
     3481 aagaacgcga agtagagccc gccgcgcccg aggcgccggc cgacgctgcc agtccgccgg
     3541 tcgcgctgcc gcactggcgc gagcgcgtcg atggcgcgct gcagatccta gtcaccacca
     3601 cccgcctgtt cctgcgcaac gaactggaga accacgccgc tgcagtcgcc ttctacttcc
     3661 tgttgtcggt ggtgccggtg gtcttcatcg tgctctggct caccggcacc gtgctgcagg
     3721 cgccggcgct ctcggcccac ttcgtcgagc tgctctcggg catcgaccaa cggctatcgc
     3781 tcgacgaact acgtgccgcc gggctgctcc cggacgccgc tgccggcacc gtcggcggtc
     3841 tgtcgctgct ggcgatgctc tgggcctcgc gccagctcgt gctggccgtg caagcggcct
     3901 tccgcgtcat caacgccgac gcccagccgc gcgccgccta cctcgactgg atggtctcgc
     3961 tgctgctggt gcccggcgca atcctggtca tcgccacggt ttcgatcggc cggcagctga
     4021 tcgcttttct cgccagcctc gccggcagcg gctggctcgc agacactgcg ccggcgctgg
     4081 caggcctggt cagccacggg ctgacgctga ccatcgtctg gctgatcgtc gtcgccgcct
     4141 tctccggcct gccgccacgg cgtccgccgg cgcgggtcgt cgttcgcgtt gcgctgctct
     4201 gcgtcctgtc gctggtggcc ctgcgcgtgc tgtttgccac cttcgtcagc ctcgaccgct
     4261 accagggcat ctacagctcg ctgggcacgc tgatcttcgt gctgttctgg gtcttcctcg
     4321 ccttcctggt gttcttcttc tgggcgcagt gcctgtacgc ggcgacccac ctcgacgcca
     4381 tcgcgctgga aaagctgatg ctctcggccg gcggcgagcg cagctggctc gaccgccacc
     4441 tctttggccg caagtcgcgg ctcacccgca agttcgggca aagctattcg gcgggacagg
     4501 tgatcgtccg cgagggtgat gaatcgcagg tcgccttcta cctcgacgag ggacacgtcg
     4561 gcctctaccg cggccacagc gacgatacgt ccggcgagcg gctcgccgcc gtcgaggccg
     4621 gcggtacttt cggcgagatg gcctacctgc tcaaggaacg acgctcggca acggccattg
     4681 ccgaaaccga cgtgtcggtt tacatcatcc cgccgacgct tttcgaggaa ctgctggcgc
     4741 tcaacccgcg actgtcgcgg cggatcatcg aaacgctggc cgagcggctg cggcagatga
     4801 acgagctgca ctgacggccg ggccgcagcc cgacggcctc agaacgactc gtcgtcgcgc
     4861 agcagccgcc agtggccgac ttccagcttg cccaggcgga tcttgccgat gcgcacgcgc
     4921 ttgaggccga cgaccttaag gccgaccagc tcgcacatgc ggcggatctg ccgcttcttg
     4981 ccttcgcgca gcacgaagcg cagctggtcc tcgttctgcc agctgacctt ggccggcttc
     5041 agcgcctgcc cgtcaagggc gaggccatgg ttgagcagct tgagcccggt ttcgtcgagc
     5101 gtgccttcga cgcggaccaa gtattccttt tcgacctgcg aatcctcgcc gatcagatga
     5161 cgggcgatcc gcccgtcctg cgtcagcacc agtaggccga ccgaatcgac gtcgagccga
     5221 ccggccgggg caaggccgcg caacatgccg gaggcaaact cgcgctcgcg cccacttcgc
     5281 tgctgcgtct cgggcttgat cagcgtcacc gccgggatgc agccgggttc gggctggccc
     5341 gagacgtagc cgaccggctt gttgagcaga atcgtcaccc gcttggcctg ctggccctcg
     5401 gccgccttct cgagcgtgat cttggcgtcg gggcgggccc gggcgccgag ctggtcgacg
     5461 atctcgccat cgacgcggac taggcccagc tcgatgtatt cgtcggcctc gcgccgcgaa
     5521 cagagtccgc gctcggccat catcttggag atgcgaatgc cttcaccgta aggcagacgc
     5581 gtcgccgact tgccgtcttc cgccgctgcc ggtggcggcg cttcctcgcg gcgcggccgt
     5641 ggggctcgag tggccgacgg tagccgctcg ctgcgcgtct cggacgccca ctcgccgcgg
     5701 ggctgcggcc gccgttcggc acgcggctcc gcgcgccact cgccgcgcga ttcccggggc
     5761 cgctcgctgc gcgactcgga agccgcgtcg aaacgctttt ccgggcggcg ttcgcgcggc
     5821 gcggctgcgc cccgcggcgg ctcggagaac ggcgacgcgg gcatcggctc aggcgcatcg
     5881 gccggcgcag cgctggacgt cgctggcggt gccgcggccg gcgtcggcga atccgcggcc
     5941 gtcactttct gttcacgcca ggcgaagggg cgcggcgcgg tctgctcgac accgaaacgc
     6001 ccggcattgc gggtatcggc agcgggacgc ggactgcgct cggcgcttgg cg
//
LOCUS       DATDAA010000075         6334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_40272,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000075 DATDAA010000000
VERSION     DATDAA010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40272"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..201)
                     /locus_tag="VIS73_02440"
     tRNA            complement(125..201)
                     /locus_tag="VIS73_02440"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(165..167),aa:Arg,seq:acg)
     gene            complement(296..577)
                     /locus_tag="VIS73_02445"
     CDS             complement(296..577)
                     /locus_tag="VIS73_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEY5762043.1"
                     /translation="MRVGVAYSEPAGQFWVEIEVDDDSTVRNAIDRSGVLQRYPHIDL
                     STQKVGVFGKTVKVEAALKAGDRIEIYRPITADPQTVKRRDNVGQDASD"
     gene            complement(580..1263)
                     /locus_tag="VIS73_02450"
     CDS             complement(580..1263)
                     /locus_tag="VIS73_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HEY5762044.1"
                     /translation="MSTSLTDITRDGLWGNNVVFVQLLALCPAMAVTTSATNGVGMGI
                     ASTAVLVCANLLVSLIRALVSPQVRIPVFIVVIATLVTVVDLVLNAFLHELHKVLGLF
                     IALIVVNCAILGRAEAFASRNRLVPSLVDGLAMGGGLTLALTAIGLCREVLGAGTVFF
                     QASALLGSSVAFLESKLPVYDGALLFILPPGGFAVLAFLLAGKRLLDARQAMRASPSP
                     ASPAVADGS"
     gene            complement(1260..1874)
                     /gene="rsxG"
                     /locus_tag="VIS73_02455"
     CDS             complement(1260..1874)
                     /gene="rsxG"
                     /locus_tag="VIS73_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HEY5762045.1"
                     /translation="MSRRLEVIRLSLAYQPVLLAVVVVLAGAALVLANRVTHEPIARA
                     EADDRRQMLSQVVPAELPDNDLLADVVTVDGPRGKPLRVHLARRDGRVVAAAFETVGR
                     GYAGDIVLLMAVDADGRVLGVRVLRHQETPGLGDKIEIAKNPWIDGFAGRTLASGGWA
                     VKKDGGEFDAFAGATITPRAVVKAVHEGLQFFQSRRNTILGAKP"
     gene            complement(1871..2977)
                     /locus_tag="VIS73_02460"
     CDS             complement(1871..2977)
                     /locus_tag="VIS73_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HEY5762046.1"
                     /translation="MDVSTLRLAKPEPAPHAHGGGTVGAIMRRVCIALVPATIYGFWL
                     YGWPAINLFCVTVGSAVLAEIFCLRLMRRASRLADGSAVLTGWLLALSLPPWAPAWIG
                     IVGGFVAIAIGKQLFGGLGFNPFNPAMVARVFLLVSFPVVMTQWVAPLPISDPAAPGI
                     VEGIRQSWQGTPPPDAVTSATLLGHVKTEFSRGVAPADAVSGAAATLQSWTGMRAGSL
                     GETAALLLVAGGLYLLALRIISWHIPLAVLAGIGVPALIAHWFDPARFLAAEVHLLSG
                     AAVLGAFFIATDYVTSPNTRSGQLVYGASIGLLTWLIRSFGGYPEGIAFAILLMNAMT
                     PLIDRTIRPRIVGRTRSGAPISVRAAAAKSGESR"
     gene            complement(2980..4536)
                     /gene="rsxC"
                     /locus_tag="VIS73_02465"
     CDS             complement(2980..4536)
                     /gene="rsxC"
                     /locus_tag="VIS73_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HEY5762047.1"
                     /translation="MAQARRPLIDRLRTAWRSPRWGVHPDDHKRPAADYPVRVMPVPK
                     RLYVPLMQHIGAPAEARVEIGQTVRTGELLAAPQGAVSAPVHAPSSGLVVDIAEVIAP
                     HPSGLTLPAVIIETDGRDRWVERESLDDPFLLSPAEISRRVAAAGVVGLGGAAFPSSV
                     KLVGGRSADVTHLLVNGAECEPYLSCDDRLMRDRAAAIIEGLRIVRFATGASEAIVGI
                     EDNKPEALAAMRDAAGIDRLIEVRSIPAHYPMGSERQLVQTLTGHEIPAGGRPSDVGA
                     LVHNVGTLLAVRHAVCDGRALISRFVTVAGGAVRAPGNIEVRIGTLAADLVDYCGGLT
                     APPARMLLGGPMMGFRVETLEVPIVKGTSGVLLLDAAELAECAPGPCIRCGNCVSACP
                     CGLLPLEMSRHVQAGDLDGAVALGLKDCLSCGCCAYVCPAHLPLVQSFHYARGELAAR
                     ARAGSKLESAKRLARARAERLEREAREKAQAAARRRAEKAAQAAAGTPAESGESSGKR
                     HREPDPAEAT"
     gene            complement(4538..5098)
                     /locus_tag="VIS73_02470"
     CDS             complement(4538..5098)
                     /locus_tag="VIS73_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEY5762048.1"
                     /translation="MLVALLILAALGTALGLVLGVASRLLAVAGDPLADEVVAMMPGT
                     NCGQCGFPGCAGAAAALIEKRAAATCCPPGGKVLAVALAAKLELPLDADAMDESGPQL
                     AEVDETICIGCCKCLKVCSTDAFIGAAKQIHNVLREACTGCGECVEICPTEAVRMVNV
                     PVTLPHWYWPKPGDPVRTALPALAGG"
     gene            complement(5118..5708)
                     /gene="rsxA"
                     /locus_tag="VIS73_02475"
     CDS             complement(5118..5708)
                     /gene="rsxA"
                     /locus_tag="VIS73_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HEY5762049.1"
                     /translation="MQELMLILLSTALVNNVVLVNFLGLCPAMGVSKGMSSAFGMGLA
                     TTFVMTLAAAATWLIEHLLLQPFDIGFLRLLTFILVIAATVQFTEMAIRKASPGLYQS
                     LGIYLPLITTNCAVLGLTLLNVKAEAGFLISVLHGFGAAMGFTLVMVMLAGLRERLAL
                     APVPAVFAGAPVALLTISLLSMAFMGFAGLIPVGGS"
     gene            6076..>6334
                     /locus_tag="VIS73_02480"
     CDS             6076..>6334
                     /locus_tag="VIS73_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762050.1"
                     /translation="MLPIDTDESTAGTSAATQHAQAVAVAPGIHWIGAFDPDLRAFDL
                     ILRTANGTSYNAYCVRGSEGVAIIDTVKEAFADDFFARLESV"
BASE COUNT         1229 a         2113 c         2021 g          971 t
ORIGIN      
        1 aagcggccac gaccaaacgg ttcctgacca gcaagcggcc aacaatgatt cccctagcgc
       61 ttcccctaga gcagaaatga gaaaggcggc tagtgaacgc tatcactaac cgccttgatt
      121 tctgtggcgc gcccggagag attcgaactc cctacccctt ggttcgtagc caagtactct
      181 atccagatga gctacgggcg cccgcgagag gtcggcattc taaccgaagg gttttggcga
      241 ctcaaccctt attttccact tactcgaatg ggcttgccgt gcccatgcag tcagcctaat
      301 cactggcatc ctgcccaacg ttgtcgcgcc gcttgacggt ctggggatcg gccgtgatcg
      361 ggcgatagat ttcgatgcga tcgccggcct tgagcgcggc ttctaccttg accgtcttgc
      421 cgaagacacc aaccttctgg gtactcaggt cgatgtgcgg atagcgctgc agaactcctg
      481 agcggtcaat ggcattgcga accgtcgagt cgtcatcaac ttcgatctcg acccaaaact
      541 gcccggccgg ctcggaatag gcaacgccga cacgcatcgt tagctcccgt ctgcgaccgc
      601 tggtgatgcc ggcgacggcg atgcgcgcat cgcctgccgc gcatcgagca agcgtttgcc
      661 ggccagcagg aaggcgagca ccgcaaatcc gccgggcggc aggatgaaca gcaacgcgcc
      721 gtcgtaaacc ggaagcttcg actcgaggaa ggcgacgctg ctgccaagca aggcgctggc
      781 ctggaaaaaa acggttccgg caccaagcac ttcgcggcac aaaccgatgg ctgtcagggc
      841 cagcgtcagc ccaccgccca ttgccagacc atcgaccagc gacggaacga ggcgattgcg
      901 actggcgaag gcctcggctc gaccaaggat cgcgcagtta acaacgatca gtgcgatgaa
      961 caggccgagc accttgtgca actcgtgcag aaacgcgtta aggaccagat cgaccacggt
     1021 caccaaggtc gcaatgacga cgatgaagac cggaattcga acctgcgggc tgaccagagc
     1081 cctgatcagc gatacgagca ggttggcgca aacgagtacg gcggtcgatg cgattcccat
     1141 acctacccca ttggtcgccg aggtagtgac cgccattgcc gggcaaagcg ccagcaactg
     1201 gacgaaaaca acgttgttgc cccacaggcc gtcgcgggtg atgtcggtca gcgatgtact
     1261 catggcttcg ctccaaggat cgtgtttcgc cggctctgaa agaactgcag gccctcgtgg
     1321 acggctttga ccaccgcccg tggcgtgatc gtcgcgccgg cgaaggcgtc aaactcgccg
     1381 ccgtccttct tgactgccca cccgccactg gccagcgttc gaccggcgaa accgtctatc
     1441 cacgggttct tggcgatctc gatcttgtcg ccgaggcccg gcgtttcctg gtgtcggagc
     1501 acccgtacac caagcacgcg cccgtcggca tcaacagcca tcagcaacac gatgtcgccc
     1561 gcgtagccac gaccaacggt ttcgaaggcg gcggcgacca cgcgaccgtc gcgacgcgcg
     1621 agatgaaccc gcagcggctt gccgcgcgga ccgtcgaccg tgacgacatc ggccagcagg
     1681 tcgttgtccg gcagttcagc gggaacgacc tgcgacaaca tctgccgtcg gtcgtcggct
     1741 tccgcgcggg cgatcggctc gtgtgtcacc cggttggcga gaaccagcgc agcgccggcg
     1801 agcacgacga cgacggcgag caggaccggc tggtaggcca gactcagccg tattacttcg
     1861 agtcgtcggc tcaccgggac tctcccgact tcgccgcggc cgcgcgcacg gaaatcggcg
     1921 caccgctgcg ggtccgccca acgatgcggg gacggatcgt acgatcgatc agcggcgtca
     1981 tcgcattcat cagcaggatg gcgaaggcga ttccctctgg gtagccaccg aaactgcgaa
     2041 tcaaccaggt aagcaggcca atcgacgcgc cgtagacgag ctgtccgctg cgggtatttg
     2101 gcgaggtcac gtagtcggtg gcgatgaaga acgcgccgag cacggcggcg cctgacagca
     2161 ggtgtacttc ggcggcgagg aaacgcgccg gatcgaacca atgcgcgatc agggccggca
     2221 cgccgatgcc ggcaaggacg gcgagcggaa tgtgccagct gatgatgcgc agcgcgagaa
     2281 ggtacaaacc tccggcgacc agcaggagcg ccgcggtctc gccgaggctg ccggcacgca
     2341 tgccggtcca agactgcaac gttgctgcgg caccggacac cgcgtcggct ggggcgacgc
     2401 cacgcgaaaa ctctgtcttc acgtggccga gcagcgtcgc cgatgtcacc gcatcgggcg
     2461 gcggcgtgcc ctgccaagac tggcgaattc cctcgacgat gccgggtgcc gctgggtcgc
     2521 tgatcggcaa tggcgcaacc cattgcgtca tcaccacggg gaacgagacg agcagaaaga
     2581 cgcgcgccac catcgccgga ttgaaggggt tgaacccgag cccgccgaag agttgcttgc
     2641 cgatcgcgat ggcgacgaag ccaccgacga tgccgatcca ggccggcgcc caaggcggca
     2701 gcgacaacgc cagcaaccag ccggtgagca ctgccgagcc atcggccaac cgagacgcgc
     2761 gccgcatcag gcgcagacag aagatttcgg ccagcacggc gctaccgacc gtaacgcaga
     2821 agaggttgat cgccggccag ccgtacagcc agaaaccgta gatcgtcgca ggcactagtg
     2881 caatgcagac gcggcgcatg atcgcgccaa cggtcccacc gccgtgcgcg tgcggcgccg
     2941 gttcgggctt ggcgagtcgg agcgtcgata cgtccatgat caggtggcct ctgccggatc
     3001 cggctcgcga tgccgtttcc ctgaagattc gcccgattcg gctggcgtcc cggccgccgc
     3061 ctgcgcagcc ttctctgccc gccgccgggc ggcggcctgg gccttttcgc gcgcttcgcg
     3121 ctcgagccgt tcggcccgcg cacgagccag ccgcttcgcc gactcgagct tgctcccggc
     3181 gcgggcgcgg gccgccagtt cgccacgcgc gtaatggaac gactgcacga gcggcagatg
     3241 ggccgggcaa acgtaggcgc agcaaccgca ggacaggcaa tccttgaggc caagcgctac
     3301 cgcaccgtcg agatcgccgg cttgcacgtg gcgggacatc tccagcggca gcaggccaca
     3361 gggacaggcc gacacacagt tgccgcagcg gatgcaggga ccgggcgcgc attcggcaag
     3421 ctcggcggcg tcgagcagca gcacgccgct ggtgcccttc acgatgggca cctcaagcgt
     3481 ctcgacgcga aagcccatca tcggcccgcc gagcaacatg cgcgccggcg gcgcggtgag
     3541 tccaccgcaa taatcgacga gatcggcggc cagtgtgccg atccggactt cgatgttgcc
     3601 cggcgcgcgc acggcgccgc cggcaaccgt aacgaaacgc gagatcagcg cccgcccatc
     3661 gcagaccgca tggcgtacgg caagcaaggt cccgacgttg tggaccagag caccgacgtc
     3721 cgaaggacga ccgccggccg ggatctcatg cccggtcaag gtctgcacga gttgccgctc
     3781 cgatcccatc gggtagtggg caggaatgct gcgaacttcg atcaaccgat cgatcccagc
     3841 cgcatcgcgc atcgccgcca gcgcctcagg cttgttgtcc tcgatgccga cgatcgcctc
     3901 gctggcgccg gtggcaaagc ggacgatgcg cagcccttcg atgatcgccg cggcgcgatc
     3961 gcgcatcagg cggtcgtcgc aggacaagta aggttcgcac tcggcgccgt tgaccagcaa
     4021 atgagtaacg tccgccgagc gcccgccgac cagcttcacc gaggacggaa acgccgcgcc
     4081 gccgaggccg accacaccgg ccgcggcaac gcgccggctg atctcggcgg gcgagagcag
     4141 aaaaggatca tcgagcgatt cacgctcgac ccagcggtcc cgtccatcgg tctcgatgat
     4201 taccgccggc agcgtcaggc ccgaaggatg cggagcgata acttcggcga tgtcgacaac
     4261 taggccagaa ctcggcgcat ggaccggcgc cgatacggcc ccctgcggag cggccagcaa
     4321 ttcgccagtg cgcacggtct ggccgatctc gacgcgcgct tcggccggag caccgatgtg
     4381 ctgcatcagc ggaacgtaga gccgcttcgg caccggcatg acacgcaccg ggtaatcggc
     4441 agcgggacgc ttgtggtcat cagggtggac gccccagcgc ggactccgcc aggcggtgcg
     4501 caggcggtcg atgaggggac gtcttgcctg cgccatgtca gcctccagcc aacgccggaa
     4561 gggcggtgcg gaccgggtcg cccggttttg gccagtacca gtgaggcaag gtgacgggca
     4621 cgttcaccat gcgcacggct tcggtcgggc agatttcgac gcattcgccg caaccggtgc
     4681 aggcttcgcg caagacgttg tgaatctgct tggccgcgcc gatgaaagcg tcggtcgagc
     4741 agaccttgag gcatttgcaa cagccgatgc agatcgtctc gtccacttcc gcgagctgcg
     4801 ggccggactc atccatcgcg tcggcgtcga gcggcagctc gagtttggcc gcgagggcca
     4861 ccgccagcac cttgccgccc ggtggacagc aggtcgccgc tgcacgtttt tcgatcagcg
     4921 ccgcagcggc accggcacag ccgggaaaac cgcactggcc gcagttggtg ccgggcatca
     4981 tcgccaccac ctcgtcggcc agcggatcgc cggcgaccgc cagcaggcgg ctggcgaccc
     5041 ccagcacgag gccgagtgcg gtaccgaggg ccgcgagaat caacagggcg acgagcatcg
     5101 tgaatccctc cgggccatca cgatccgccc accggaatca gcccggcgaa acccataaag
     5161 gccatcgaca acaggctgat cgtcaacaag gccaccggcg caccggcaaa aaccgcgggc
     5221 accggcgcca gcgccaggcg ttcgcgcaga cctgcaagca tcaccatcac cagcgtgaag
     5281 cccatcgctg cgccaaaacc gtggagaacg ctgatgagga aacccgcctc ggccttgacg
     5341 ttaagcagcg tcaggccgag cacggcgcag ttggtggtta tcaacggcag gtagatgccc
     5401 aggctctggt ataggccggg actcgccttg cggatcgcca tttcggtgaa ctgcacggtt
     5461 gctgcgatca ccaggatgaa ggtcagtaac ctgaggaagc cgatgtcgaa gggctgcagc
     5521 agaaggtgct cgatcagcca ggtcgccgcc gcggcgagcg tcatcacgaa ggtggtggcg
     5581 agacccatgc cgaacgccga ggacattccc ttggaaaccc ccatcgccgg acacaggccg
     5641 agaaagttca ccagcacgac gttgttcacg agtgcagtcg agagcaggat cagcatcagt
     5701 tcttgcatgg cgtgttttgg gcggtcttcg ctcgtcgtcg cgtgccagtt ttcggggtac
     5761 cggttccttg cggcctgcgt ttcctacatt tgccgcaaca ctgcaggcat cgcgatggcg
     5821 tctttgcagg gagcgtgcca gtgcgcgcga catcggcgcc aatcgcccga ctgctgagcc
     5881 ttcgcgcgga ttcgtcgatc ccggctgctt gctcagcagt cggctgcctg tcgcaaagcc
     5941 gacacgcgtg gcgcccaatc gctgtcgcgc acatcacatt ggccttcgcc ccgcaattaa
     6001 gtgaagccaa ttcaatcgct tgtggactgg caccggtctt gctgtggatg cagcaccgaa
     6061 accggatgcc ctgccatgct tcccatcgac actgacgaat ccactgccgg cacctctgcc
     6121 gccacgcagc acgcgcaggc agtcgccgtc gcgccgggaa tccactggat tggggccttc
     6181 gatcccgacc tgcgcgcctt cgatctgatc ctccgtaccg ccaacgggac cagctacaac
     6241 gcctattgcg tgcgcggaag cgagggcgtg gcgatcatcg acacggtcaa ggaagccttc
     6301 gccgacgatt tctttgcgcg cctcgaatcg gtct
//
LOCUS       DATDAA010000076        14698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_494552,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000076 DATDAA010000000
VERSION     DATDAA010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 14698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14698
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494552"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VIS73_02485"
     CDS             complement(<1..542)
                     /locus_tag="VIS73_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEY5762051.1"
                     /translation="MKLAHTLALAAALLLPATVGAESAPTADAAKAVPEAAVAQAIKA
                     STADHARFKQLDRDFKSGPELTRACLECHTEAAKQVHGSKHWNWQFTNPTDGKTVGKK
                     NMVNNFCTATTSNEKGCTACHIGYGWQDNVSFDFKSEENVDCVVCHDTTGTYRKLPGD
                     AGNPLIEKKEFPPKSGKFVGP"
     gene            complement(554..1105)
                     /locus_tag="VIS73_02490"
     CDS             complement(554..1105)
                     /locus_tag="VIS73_02490"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEY5762052.1"
                     /translation="MLKAIGGFFAAMVLAGIGAWQFGGSLMFNETPSPFGMEETVARI
                     QHNIQGTGNGWVLSGLRNPARAVQADGGNTLPVMMVEACSTKYSGPILNDDATRILSI
                     LMPCKITVYKKNDGKIYVGTMNAGLMGKLFGPTVSEIMGKVAQDQKKFLVFDPSTPAP
                     PMILPTAAGGGAGGSGAGGGSGC"
     gene            complement(1283..1555)
                     /locus_tag="VIS73_02495"
     CDS             complement(1283..1555)
                     /locus_tag="VIS73_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY5762053.1"
                     /translation="MGRPVEIRIPRFPECWEACGSCGQGEVLIDDICALPGDRVERDD
                     TLVVLETGKVALDIPSPHTGVLLELHVAAGDSVGEGALIAVIDADD"
     gene            1635..1892
                     /locus_tag="VIS73_02500"
     CDS             1635..1892
                     /locus_tag="VIS73_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762054.1"
                     /translation="MIHACTANTDLDELIGRETHDGPQRQFAYGPLAMAMKQDEELVL
                     ENSAALSLFMRAKLGALINDLFIGDTAETLHAGTGFRVVLR"
     gene            complement(2030..2548)
                     /locus_tag="VIS73_02505"
     CDS             complement(2030..2548)
                     /locus_tag="VIS73_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY5762055.1"
                     /translation="MSRQSLILTVIADDRPGLVGELSAAVSAHKGNWLESSLAQLAGK
                     FAGIVRVEVDEVDAGSLQATVERLPNLRVSVTRAGTAAAPAGRRLTLELVGNDRVGIV
                     KEVTAVLARHSVNVEKLETRTESGPMAAGMLFRAEADLTAPAALDARTLKGDLEAISH
                     DLMVEIVLGERM"
     gene            complement(2548..5430)
                     /gene="gcvP"
                     /locus_tag="VIS73_02510"
     CDS             complement(2548..5430)
                     /gene="gcvP"
                     /locus_tag="VIS73_02510"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEY5762056.1"
                     /translation="MLNPTLNLPLSQLEQREDFIARHIGPDETDVAAMLATLGTPSLE
                     ALIDQTVPTAIRLGKPLAIDDAMTEHEALAKLKSIAAKNVVKRSLIGLGYYDTTMPTV
                     ILRNVLENPGWYTAYTPYQAEIAQGRLEALLNFQQMVIDLTGLELANASLLDEATAAA
                     EAMTMARRVSKAKANAFFVDAGCFPQTIDVVKTRAGFFGFEIVVGDAAGAAKGDYFGA
                     LLQTPNAQGEAPDLTEVIAELKAKGTVVAVASDLMALVLMKSPGEMGADIALGSSQRF
                     GIPMGFGGPHAAFFATHEANVRSMPGRIIGVSKDAHGRKALRMTLQTREQHIRREKAN
                     SNICTSQVLLANLAGMYAVYHGPKGLQAIAGRIHRLAAILATGLKATHAKVCNESYFD
                     TLTIEVGAKAEAIYTAAQAAGYNLRRASETTLALSLDEKTTRNDVATLLKLFTGATAD
                     VATLDAMLPCGLPAGVQRHTAILQHPVFNTHHSEHEMLRYLKRLQNKDLALDHSMISL
                     GSCTMKLNATSEMIPVTWREFSDMHPFAPRAQCAGYLEMIDGLSAYLKAITGFDAINM
                     QPNSGAQGEYAGLVAIARYHESRGEGHRNVCLIPKSAHGTNPATAQMCNMEVVVVACD
                     ESGNVDLADLTAKAEQHADKLACLMLTYPSTHGVFEEAVKDVCAVVHKHGGQVYMDGA
                     NLNAQVGLTSPAAIGADVSHMNLHKTFAIPHGGGGPGMGPIGMKAHLAPFMAGHVFAP
                     TDADTVGTMGAVSAAPFGSASILNISWMYIAMMGGKGLTRATEVAILNANYVASRLAS
                     HYPVLYKGKKGRVAHECILDVRAIKAATGIAEIDIAKRLMDYGFHAPTVSFPVAGTLM
                     VEPTESESRAELDRFCDAMISIREEIRAIERGDYRADDSPLKHAPHTQGDVIGAEWVR
                     PYTPEKAVFPLPWVAENKFWPSVNRIDDVYGDRNLHCSCVPMEEYQ"
     gene            complement(5445..5834)
                     /gene="gcvH"
                     /locus_tag="VIS73_02515"
     CDS             complement(5445..5834)
                     /gene="gcvH"
                     /locus_tag="VIS73_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY5762057.1"
                     /translation="MSNVPSNLKYTKSHEWVRAESDGTITVGITDHAQQLLGDLVFVE
                     LPEVGKTLDAGQEACVVESVKAAADAYAPVAGEVVAVNEAVPDAPESVNQDAFAAWLF
                     RLKPANAADLGTLLDATAYQAVVDTES"
     gene            complement(5858..6976)
                     /gene="gcvT"
                     /locus_tag="VIS73_02520"
     CDS             complement(5858..6976)
                     /gene="gcvT"
                     /locus_tag="VIS73_02520"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY5762058.1"
                     /translation="MPEKTPLQQTPLNAAHRAMGARMVDFGGWDMPVNYGSQIEEHHA
                     VRRDCGMFDVSHMCAVDIRGADARAFLGRLVANNVDKLKVPGKALYSAMLNAKGGVVD
                     DLIIYFLSDTDFRLVINAGTAEKDLAWMEARRGEWGMRLTITERRSANEPLAMIAVQG
                     PNARARVWQVLPQTKAVTESFGPFFGAQIDQYFIATTGYTGEDGFEIALPAAEAETLW
                     QGLAAAGVKPCGLGARDTLRLEAGMNLYGQDMDESVSPLDAGMGWTVDLKTERDFVGR
                     PALEQNGKRAQLLGLVLQDKGVLRGHQKVFCRVNGTEAVGEITSGSFSPTTERSIALA
                     RLPLGVVVGDDVEVEIRNKRLKAMVVKPVFARNGKALI"
     regulatory      complement(7042..7147)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(7154..7259)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(7333..9057)
                     /locus_tag="VIS73_02525"
     CDS             complement(7333..9057)
                     /locus_tag="VIS73_02525"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5762059.1"
                     /translation="MPNGPQSPAEIARETLRKLAAERLQPTPDNFRSQYHRIAGTTAD
                     EAFPSHMLGAIVAELPRSNAAALKVVHDFERAIASSQWPTLRATLHELVRMATETPPA
                     WGALIRQIVNGLDGEAPGADPVARRRELQRVLGGNHADTQVLFARLRGLANDWSKSAA
                     ADETETNGNGINGAIGFGSLAPDLARLIWRGVMPMAAGHPAIADELDQLASVLGKPVD
                     EEQLATIAAQLESLTGKLEWLGEDQRAIRETLLGLLRLIVDNIGELVLEDSWLHGQIA
                     AISQCFEGPLDIRVLDDIERRLREVIAKQSRLKRHLSEAQTRLQSMLAGFLERLAGIK
                     ETTGSYQATLSQCAKQIADAKSLDELSSVVENVLRETQAVHETTTRAGVELDELKSQV
                     EAANLRIENLQRELDETSELVRHDSLTGTLNRRGIDEALIREAARCRRRGEALCIAVL
                     DIDGFKHVNDTFGHQTGDAALQHLVQVTRECLRPPDIVGRYGGEEFVILLPETTAAEA
                     ATVLTRLQRELTRRYFLADNQRLLITFSAGIAQLNDGEETQAAIDRADRAMYAAKRAG
                     KNRVLLAG"
     gene            9303..10130
                     /locus_tag="VIS73_02530"
     CDS             9303..10130
                     /locus_tag="VIS73_02530"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HEY5762060.1"
                     /translation="MNWDFHCHSSVSDGLLPPAQVVARAAANGVDLLALTDHDAIDGL
                     AAADAAAREAGIRLLPGVEISIEWQGTPIHIVGLGIDASCAVLVEGLASLREGRTERA
                     RRMSAALEKVGIPGAFEGALRYAANPSLVSRAHFARFLVEHGVCDEVKRVFDRYLVPG
                     KPGYVEHQWATLADATGWIAAAGGIAVVAHPARYKLTNGATRRFLEDFIAHGGQGIEV
                     VCGSHTPDNVRQFARLATDLGLYASRGSDFHGPEESVVDLGRMPELPAGQRPVLELL"
     gene            10137..10763
                     /locus_tag="VIS73_02535"
     CDS             10137..10763
                     /locus_tag="VIS73_02535"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY5762061.1"
                     /translation="MAQYFGIHPDNPQPRLLQQAAEIIRGGGIVALPTDSSYALACHT
                     GDKDALERLRQIRGIDDRHHLTLMCRNLADIGQFARVDNACYRLLKSATPGSYVFILE
                     GTRELPRRILHPKRKTIGLRIPDHRVIQALLEVLGEPLLTTTLQLPGDESPMTDAWEI
                     RERLDKLVDLVLDGGSCGTDSTTVIDLTGQSPEVVRAGRGPLEPLGLA"
     gene            10793..11446
                     /locus_tag="VIS73_02540"
     CDS             10793..11446
                     /locus_tag="VIS73_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY5762062.1"
                     /translation="MTEIIQTISLALLPVTLAITLHEAAHGYAARYFGDPTAHLAGRI
                     TLNPIRHIDPIGTIAVPLLILLVSGGGMLFGWAKPVPVDFGRLRKPKRDMTWVAGAGP
                     AANLLMAVFWAAMLKLAWAMPENAYTMPLALMSQIGISINVVLMVLNLLPLPPLDGGR
                     IAVSLLPHEMAWRFAKLERWGFPILLVLLFTGVLGAVLFPLVRLVRMAIAGLFGFPG"
     gene            11463..12665
                     /locus_tag="VIS73_02545"
     CDS             11463..12665
                     /locus_tag="VIS73_02545"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY5762063.1"
                     /translation="MYAERVLSGMRPTGALHLGHYHGVLKNWVRLQAEHPCLFFVADW
                     HALTTHYDDPAGIEASVWDMVVDWLAAGVDPGQSTIFIQSQVPEHAELHLLLSMMTPL
                     GWLERVPTYKDQQEKLEQKDLSTYGFLGYPLLQSADILIYRANRVPVGEDQVPHIEFS
                     REIARRFNHLYGREPGFEDKAREAVKRLGSKKARYYNNLRTRYQEQGDASAIEQAHAL
                     LDEIQHVSVADRERLFGYLEGTGKMILVEPEAMLTAASKMPGLDGQKMSKSYNNTISL
                     REDPESVRQKVRRMPTDPARVRRTDVGDPERCPVWQLHQVYSNDEVKQWVREGCTSAG
                     IGCLDCKEPVIEGMLAELAPIQERARAYLDDPTTVRNVVADGCERARRLAHETMRDVR
                     ESMGLHYD"
     gene            12693..13217
                     /locus_tag="VIS73_02550"
     CDS             12693..13217
                     /locus_tag="VIS73_02550"
                     /inference="COORDINATES: protein motif:HMM:NF008675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY5762064.1"
                     /translation="MSAREMPLEKEVRRVLASGASPQDIEAELLAMIHDVFANRIPFN
                     KVLGLDIISLDGDRPVLGFPMRDELVGNFFHGILHGGVTSAVLDVTGGIVAFLGLQRK
                     LVDLPIAERLERFSKIGTIDLRIDYLRPGKGKSFTATGYALRTGNKVAVARMELHNDS
                     NDLIAVGTGAYLVG"
     gene            13350..14144
                     /locus_tag="VIS73_02555"
     CDS             13350..14144
                     /locus_tag="VIS73_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEY5762065.1"
                     /translation="MIYGEPLMQLPLDLYIPPDALAIMLESFEGPLDLLLYMIRKQNV
                     DILDIPMASLTRQYLEYVEAMRSQNLELAAEYLVMAAMLIEIKSRMLLPRPKAAEGEE
                     TGDPRAELVRRLIEYEQMKLAAQKVNELPQADREFQWVSVLVEKSAVERLPDVNLDDL
                     RSAWLGIVRQAKLHAHHHVRREELSVREHMGLILRHLKSARSDGHGFVEFALLFKPEL
                     GVPGVVVNFLAMLELAREHLIEVTQSEAFAPIYVRSAEGEPDVITA"
     gene            14128..14685
                     /gene="scpB"
                     /locus_tag="VIS73_02560"
     CDS             14128..14685
                     /gene="scpB"
                     /locus_tag="VIS73_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY5762066.1"
                     /translation="MSSLPENPDRMKRVLEAALLAAQQPMTLVELARMFDEDVSADLL
                     RKLLDEIRDAWAESAIELVQLASGWRFRTRGEFLPYLDRLNPDKPPKYSRAVLETLAI
                     IAYKQPVTRGDIESIRGVTVGSPVIKTLEERGWIDVVGHRDTPGRPALFATTRKFLDD
                     LGLRSLTELPPLEEVAPLLENQDVA"
BASE COUNT         2333 a         4581 c         5012 g         2772 t
ORIGIN      
        1 gggccgacga acttgccgct cttgggtgga aattccttct tctcgatcag tgggttgccg
       61 gcgtcgccgg gcagcttccg ataggtgccg gtggtgtcgt ggcagaccac gcagtcgacg
      121 ttctcttcgc tcttaaagtc gaaggagacg ttgtcctgcc aaccgtagcc gatgtggcag
      181 gcggtgcagc ctttttcgtt tgaagtggtc gcggtgcaga agttgttcac catgttcttc
      241 ttgccgaccg tcttgccgtc cgtgggattg gtgaactgcc agttccagtg cttggagccg
      301 tggacctgct tggctgcctc ggtgtggcat tcgaggcagg cgcgggtcag ctcggggccg
      361 ctcttgaagt cgcggtcgag ctgcttgaag cgcgcgtggt cggcggtcga ggccttgatg
      421 gcctgggcga cggccgcctc gggtacggct ttcgccgcgt cggcagtggg cgcagactcg
      481 gcgcccacgg tggctggcag cagcagggct gcagccagcg cgagggtatg tgcgagtttc
      541 atggcggacc tcctcagcag ccgctgccgc cgccggcgcc cgatccgccc gcgccgccgc
      601 cggcggcggt gggcaggatc atcggcgggg ctggtgtgct cggatcgaag accagaaatt
      661 tcttctggtc ctgcgcgacc ttgcccatga tttcggaaac cgtcgggccg aacagcttgc
      721 ccatcaggcc ggcattcatc gttccgacgt agatcttgcc gtcgttcttc ttgtagacgg
      781 tgatcttgca gggcatgagg atcgacagga tgcgcgtcgc gtcatcgttg aggatcggcc
      841 cggaatactt ggtcgagcag gcctcgacca tcatcaccgg cagcgtgttg ccgccgtcgg
      901 cttgcacggc gcgcgccggg ttgcgcaggc ccgagaggac ccagccgttg ccggtgccct
      961 gaatgttgtg ttgtatgcgg gcaacggttt cctccatgcc gaaggggctg ggggtttcgt
     1021 tgaacatcag gctgccgccg aactgccagg caccgattcc ggccagaacc atcgccgcga
     1081 aaaatccgcc aattgctttc aacatgatcg ccctcccaaa gacgttagcg tgtagtaata
     1141 cagaaaatta gtgattacta aactactagc cggggcaagg gagggcaagc ccttcggccg
     1201 agggaatttg atctggcgca agccagcagg ggcgctttgt gacctgggtt acgaagctgg
     1261 cgctggagcg accgcgcgag cctcagtcgt cggcgtcgat gaccgcgatg agggcgcctt
     1321 cgccgaccga atcgcctgcg gcgacgtgca gttcgagcag gacgccagta tggggcgaag
     1381 gaatatccag cgcgaccttg cccgtttcga gtacaacgag cgtatcgtcg cgctccacgc
     1441 ggtcgccggg cagggcgcag atgtcatcga tcagcacctc gccttggccg caggagccgc
     1501 aggcctccca gcattccgga aagcgcggga tgcggatctc aacgggacgg cccacgatac
     1561 ctccggtgga tggctggcat aaagtttgcg atagaacgac cgtaatggcg catagggtac
     1621 cgacggagga caagatgatc catgcctgta ccgcaaacac cgacctcgac gaactgatcg
     1681 gtcgggagac tcacgatggt cctcagcgcc aatttgccta tggcccgctg gcgatggcga
     1741 tgaagcagga cgaagagctc gtgctcgaaa acagcgcggc gctctccctc ttcatgcgcg
     1801 ccaagctcgg tgcgctgatc aacgacctgt ttatcggcga tacggccgag acgctccacg
     1861 ccggcacagg tttccgcgtc gtgctgcgct gagggtcgtc accggcaatc gctgccggtg
     1921 cgagttcggg gtcgccgcgc gcaggcggcc gctgcccgaa agaccccggc aggggcaaag
     1981 tcaccatcga atgcggggaa taaggggcag attccccttt ccccaccgcc tacatccgtt
     2041 cgccgagcac gatctcgacc atcaggtcgt gcgaaatcgc ttccagatcg cccttgagcg
     2101 tgcgcgcgtc gagggccgcc ggcgcggtca ggtcggcttc ggcgcggaac agcatgccgg
     2161 cggccatcgg cccgctttcg gtgcgggttt cgagcttttc gacgttgacc gaatggcggg
     2221 cgagcacggc ggtgacttcc ttgacgatgc cgacgcggtc gttgccgacc aactcgagcg
     2281 tcagccgccg cccggccggt gctgcggcgg tgccggcgcg ggtgaccgag acgcgcaggt
     2341 tgggcaggcg ctcgacggtc gcctgcagcg agccggcgtc gacctcgtcg acctcgacgc
     2401 ggacgatgcc ggcgaacttc ccggccagtt gtgccagcga ggattcgagc cagttaccct
     2461 tgtgcgcgga aacggcggcg gagagctcgc cgacgagtcc ggggcgatcg tcggcgatga
     2521 cggttaggat cagcgattgt ctggacatta ctggtactcc tccatcggca cgcagctgca
     2581 gtgcagattg cggtcgccgt agacgtcatc gatgcggttg acgctcggcc agaacttgtt
     2641 ttcggcgacc cagggcagtg ggaagacggc cttttccggc gtgtaggggc gaacccactc
     2701 ggcgccgatg acgtcgcctt gcgtatgcgg agcgtgtttc aacgggctgt cgtcggcgcg
     2761 ataatcgccg cgctcgatgg cgcggatttc ctcgcggatc gaaatcatcg cgtcgcagaa
     2821 gcggtcgagc tcggccctcg attccgactc ggtaggctcg accatcagcg tgccggcgac
     2881 cgggaagctc accgtcggcg cgtggaagcc gtagtccatc agccgcttgg cgatgtcgat
     2941 ctcggcgatt ccggtggcgg ccttgatcgc ccgcacgtcg agaatgcact cgtgtgcgac
     3001 tcggcctttc ttgcccttgt agagcaccgg gtagtggctc gccagtcggc tggcgacgta
     3061 gttggcgtta aggatcgcca cctcggtggc gcgggtcagg cccttgccac ccatcatcgc
     3121 gatgtacatc cacgagatgt tgagaatcga cgccgaaccg aagggcgcgg ctgagaccgc
     3181 gcccatcgtg ccgaccgtgt cggcgtcggt gggggcgaag acatggccgg ccatgaaggg
     3241 cgcgaggtgc gccttcatgc cgatcgggcc catgcccggt ccgccgccgc cgtgcggaat
     3301 ggcgaaggtc ttgtgcaggt tcatgtggct gacgtcggcg ccgattgccg ctggcgaggt
     3361 caggccaacc tgcgcattga ggttggcgcc gtccatgtag acctgcccgc cgtgtttgtg
     3421 gacgaccgcg cagacgtcct tgaccgcttc ctcgaagacg ccgtgcgtcg acgggtaggt
     3481 cagcatcagg caggcgagct tgtcggcgtg ctgttcggcc ttggccgtga ggtcggcgag
     3541 atcgacgttg cccgactcat cgcaggcgac aaccacgact tccatgttgc acatctgtgc
     3601 ggtcgccggg ttggtgccgt gcgccgactt gggaatcaga cacacgttgc ggtggccttc
     3661 gccgcggctc tcgtgatagc gggcgatggc gaccaggccc gcatactcgc cctgcgcgcc
     3721 cgagttgggt tgcatgttga tcgcgtcgaa gccggtgatc gccttgaggt aggccgacag
     3781 gccgtcgatc atttccagat agccagcgca ctgggcgcgc ggcgcgaacg ggtgcatgtc
     3841 ggaaaactcg cgccaggtga ccggaatcat ttcgctggtg gcgttaagct tcatcgtgca
     3901 gctgccgagc gagatcatcg aatggtcgag cgccagatcc ttgttctgca ggcgtttcag
     3961 gtagcgcagc atctcgtgct cgctgtggtg cgtgttgaac accgggtgct ggaggatggc
     4021 ggtgtggcgc tggacgccgg cggggaggcc gcagggcagc atggcgtcga gcgtggcgac
     4081 gtcggcggtg gcgccggtga acagcttgag cagcgtcgcc acatcgttgc gcgtggtctt
     4141 ctcgtcgagc gagagcgcca gcgtggtttc cgaggcgcgg cgcaggttgt aaccggcagc
     4201 ctgcgcggcg gtgtagatcg cctcggcctt ggcgccgacc tcgatggtca gcgtgtcgaa
     4261 atagctctcg ttgcagacct tggcgtgtgt cgccttgagg ccggtggcaa ggatcgcggc
     4321 gaggcggtgg atgcgcccgg cgattgcctg caggcctttc ggcccgtgat agacggcgta
     4381 catgccggcg aggttggcga gcagcacctg cgaggtgcag atgttcgagt tggccttctc
     4441 gcggcggatg tgttgttcgc gtgtctgcag ggtcatgcgc agcgccttcc tgccatgcgc
     4501 atccttggag acgccgatga tgcggccggg catcgagcga acattggcct cgtgcgtggc
     4561 gaagaaggcc gcgtgcgggc cgccgaagcc catcgggatg ccgaagcgct gcgaggagcc
     4621 gagcgcgatg tcggcgccca tctcgccggg cgatttcatc agtaccagcg ccatcaggtc
     4681 ggaggcgacc gcgacaacgg tgcccttggc cttcagctcg gcgatcacct cggtgaggtc
     4741 gggcgcctcc ccctgcgcat tgggcgtttg cagcagcgcg ccgaagtagt cgcctttggc
     4801 tgccccggcg gcgtcgccga cgacgatctc gaagccgaaa aagccggcgc gcgttttcac
     4861 cacgtcgatg gtctgcggga agcagccggc gtcgacgaag aaggcgttgg ccttggcttt
     4921 cgagacacgg cgcgccatgg tcatcgcttc ggcggcggcc gtggcttcgt cgagcagtga
     4981 ggcgttggca agttccagac cggtgaggtc gatcaccatt tgctggaagt tgagcaacgc
     5041 ctccaggcgt ccctgcgcga tttctgcctg gtacggcgta taggcggtgt accagcccgg
     5101 attctcgagc acgttgcgca agatcaccgt tggcatggtc gtgtcgtagt agccgaggcc
     5161 gatcagcgaa cgcttgacga cgttcttcgc ggcgatgctc ttcagtttcg cgagtgcttc
     5221 gtgttcggtc atcgcatcgt cgatcgccag gggcttgccg aggcggatcg ccgtcggcac
     5281 cgtttgatcg atcagtgcct cgagcgaggg cgtgccgagc gtggccagca tcgccgcgac
     5341 gtcggtctcg tcgggaccga tatggcgggc gatgaagtct tcgcgctgct cgagctgact
     5401 gagcggcagg ttgagggtcg ggttgagcat gggcgggggc tttcctacga ttcggtgtcg
     5461 accacggcct gataggcagt ggcatcgagc agcgtgccga ggtcggcggc gttggcgggc
     5521 ttcaggcgaa acagccaggc ggcaaaggcg tcttggttta ccgactccgg cgcatcgggg
     5581 accgcctcgt tcaccgcgac gacttcgccg gcgaccggcg cgtaggcatc ggcggcggcc
     5641 ttcactgatt cgacgacgca ggcttcctgg ccagcgtcta gcgtcttgcc gacttccggc
     5701 agttcgacga agacgagatc gccgagcagt tgctgcgcgt ggtcggtgat gccgacggtg
     5761 atcgtgccgt ccgattcggc gcggacccac tcgtgggatt tggtgtactt gaggttgctg
     5821 ggaacgttcg acatgtctat ctcctgagtg ggtttgttca gatcaatgct ttgccgttgc
     5881 gggcgaatac gggtttgacg accatggcct tgagccgctt gttgcggatt tccacctcga
     5941 cgtcgtcgcc gaccacgacg cccagcggca ggcgggccag cgcgatcgac ctctcggtgg
     6001 tcggcgagaa cgagcccgag gtaatctcgc cgaccgcctc ggttccattg actcggcaga
     6061 acaccttctg gtgtccgcgt agcacgccct tgtcctgcag cacgaggccg agtaactgag
     6121 ctcgtttgcc gttctgttcg agcgccgggc ggccgacgaa gtcgcgctcg gtcttgaggt
     6181 cgacggtcca gcccatgccg gcgtcaagcg gcgagaccga ctcatccatg tcttggccgt
     6241 agaggttcat gccggcttcg aggcgcagcg tgtcgcgggc gccgaggccg cagggtttga
     6301 cgccggcggc agcgagcccc tgccagagtg tttcggcctc ggcggccggc agcgcgatct
     6361 cgaagccgtc ctcgccggtg tagccggtag tggcgatgaa gtactggtcg atctgcgcgc
     6421 cgaagaaggg gccgaaggat tcggtaacgg ccttcgtctg cggcagcacc tgccagaccc
     6481 gggcacgggc attggggccc tggacggcga tcatcgctag cggctcgttg gccgaacggc
     6541 gctcagtgat cgtcagcctc atgccccatt cgccccggcg cgcttccatc cacgccagat
     6601 ccttctccgc cgtgccggcg ttgatgacca ggcggaagtc ggtgtcgctg aggaagtaga
     6661 tgatcaggtc atcgacgacg ccgcctttgg cattgagcat cgccgagtac agcgccttgc
     6721 cgggcacctt aagcttgtcg acgttgttgg cgacgagacg cccgaggaag gcgcgggcgt
     6781 cggcgccgcg gatgtcgacg gcgcacatgt gcgagacgtc gaacatgccg cagtcacggc
     6841 gcacggcgtg gtgctcctcg atctgcgagc cgtagttgac cggcatgtcc cagccgccga
     6901 agtcgaccat gcgggcgccc atcgcccggt gggcggcgtt gagcggtgtt tgctggagcg
     6961 gggttttctc tggcatttca atcccttcgg gcggttatct caatcaggtg acacgaacaa
     7021 aaacggggcg agccgaaacc ggctcacccc tctgtccttg gtacctgaga gtttcgacag
     7081 ttccgcagaa ccatcggtgc cccatcggtg gatgaccctg cggtcatcgc tctccagagt
     7141 gacagcctta cggctgttcc gggcggtcca ctttgcctga gcgattgcgg gtgtcgttgc
     7201 gccttcggcg gcatgcggga ggctcgttgg cctccgatgc gctctcccga ccggagcggc
     7261 gactatacag aggcgccgtc gcagcgacaa ccggtgacga agcggcgtcg cgcggtgccc
     7321 ctggcctggc gttcagccgg caagcagcac gcggttcttg ccggcgcgct tggcggcgta
     7381 catcgcccgg tcggcgcggt cgatagcggc ttgtgtctcc tcgccatcgt tgagctgggc
     7441 gatgccggcg ctgaaggtga tcagcaggcg ctggttgtcg gcgaggaaat agcgtcgcgt
     7501 cagctcgcgc tgcaggcggg tcagcacggt cgcggcctcc gcggcagtgg tttccggcag
     7561 cagaatgacg aattcctcgc cgccgtagcg gccgacgatg tcgggcgggc gcaggcattc
     7621 gcgcgttacc tgcaccagat gctggagcgc ggcgtcgcct gtttggtgtc cgaaggtgtc
     7681 gttgacgtgc ttgaagccgt cgatgtcgag tacggcaata cacagcgctt cgccgcgccg
     7741 ccggcagcgc gctgcttcgc ggatcagggc ctcgtcgatg ccgcggcggt tgagggtgcc
     7801 ggtcagcgaa tcgtggcgga ccagttcgct ggtctcgtcg agttcacgct gcaggttctc
     7861 gatgcgcagg ttcgcagcct cgacctgtga cttgagttcg tcgagttcga cgccggcccg
     7921 ggtcgtcgtc tcgtgtacgg cctgggtctc gcgcagcacg ttttcgacga ccgaggacag
     7981 ctcgtcgagg ctcttcgcgt cggcgatctg tttggcgcac tggctcagcg tcgcttgata
     8041 tgagccggtg gtttccttga tcccggccag ccgttctaga aagccggcga gcatcgactg
     8101 caggcgcgtc tgggcttcgc tcaggtggcg cttgaggcgc gactgcttgg cgatgacctc
     8161 gcgcagacgg cgttcgatgt cgtcgagcac gcgaatgtcg agcggtccct cgaagcactg
     8221 gctgatcgcg gcgatctgcc cgtgcagcca gctgtcttca aggaccagtt cgccgatgtt
     8281 gtcgacgatc aatcgcagca ggccgagcag tgtttcgcgg atcgcgcgct ggtcctcgcc
     8341 cagccattcg agcttgccgg tcagcgactc gagctgtgcg gcgatggtag cgagttgctc
     8401 ctcgtcgact ggtttgccga gcacgctggc gagctggtcg agttcgtcgg cgattgccgg
     8461 gtggccggcc gccatcggca ttacgccgcg ccagatcagg cgggccagat caggcgcaag
     8521 gctgccgaag ccaatggcgc cattgatgcc gttgccgttt gtctccgttt cgtcggctgc
     8581 ggctgacttc gaccagtcgt tggccaagcc gcgcagccgg gcaaagagta cctgtgtgtc
     8641 agcgtggttt ccgccgagca cgcgttgcag ctcgcgccgg cgggcgaccg ggtcggcgcc
     8701 gggtgcctcg ccgtcgaggc cgttgacgat ctggcggatc agcgcgcccc aggccggtgg
     8761 tgtttcggtc gccatgcgca cgagttcgtg gagcgtcgcg cgcagcgtcg gccactggct
     8821 gctggctatc gctcgttcga agtcgtggac gaccttgagc gcggccgcat tgctgcgcgg
     8881 cagttcggcg acgatcgcgc caagcatgtg gctcggaaac gcctcgtcgg cggtcgtccc
     8941 ggcaatccgg tggtattggc tgcggaagtt gtccggcgtt ggctgcagcc gctcggcggc
     9001 gagcttgcgc agggtttcgc gggcgatctc ggcggggctc tggggtccgt tgggcatcga
     9061 atcactggat ttgcattttt ttcgcagtat cgggcgccaa agatgcgaac gtcaattggc
     9121 gcgaggtcgc tgtgctagac tccgcgccct cgtccagatg ccgtccgtgc gaatcaattc
     9181 cggattccgg cgtcttacgt gtgatcgcat tgtgtgcagg gcggcgtagc gcgatcagac
     9241 cgcccgcgcg ttttgcggcg gcggcatgcc gatagcggca tcggccatag gctggactcc
     9301 atatgaactg ggactttcac tgtcattcgt cggtctccga cggccttttg ccgccggcgc
     9361 aggtcgttgc gcgcgccgcg gcgaatggtg tcgacttgct ggcgctcacg gaccacgacg
     9421 cgatcgacgg cctcgccgct gccgatgcgg cggcgcgtga agctggcatc cgactgctgc
     9481 cgggtgtcga gatctcgatc gagtggcagg gcacgccgat ccatatagtt ggcctcggca
     9541 ttgacgccag ttgcgcggta ctcgtcgagg gtcttgcatc gctgcgcgag gggcgtaccg
     9601 agcgcgcccg ccggatgtcg gccgcgctgg agaaggtagg cattcccggg gccttcgagg
     9661 gtgccctgcg ctacgcggcg aatccgtccc tggtctcgcg cgcgcacttt gcgcgctttc
     9721 tggtcgaaca cggcgtctgc gacgaagtga agcgtgtctt cgaccgctat ctggttccgg
     9781 gaaaaccggg ctacgtcgag caccagtggg cgacgctggc cgatgcgacg ggctggatcg
     9841 ccgctgccgg cggtatcgcc gtggtggcgc atccggcgcg ctacaagctg acgaacggcg
     9901 cgacccgccg tttcctagag gatttcatcg cccatggcgg ccagggcatc gaggtcgtct
     9961 gtggtagcca tacgcccgac aacgtccgcc agtttgcgcg gctcgctacc gatctgggcc
    10021 tgtacgcctc gcgcggctcc gacttccatg gccccgagga aagcgtcgtc gacctcgggc
    10081 ggatgcctga actgcccgcg ggccagcgac ccgttctcga actgctctga gcgcgcatgg
    10141 cccagtactt cggcattcac cctgacaacc cgcagccgcg cctgctgcag caggccgccg
    10201 agatcattcg cggcgggggc atcgtcgcgc tgccgaccga ttcgagctac gcgctcgcct
    10261 gccacaccgg cgacaaggat gcgctcgagc gcctgcggca gatccgcggc atcgacgacc
    10321 gccatcacct gacgctgatg tgccgcaatc tcgccgacat tggccagttc gcgcgtgtcg
    10381 acaatgcctg ctaccggttg ctcaaatcgg cgacgccggg ctcctacgtg ttcattctcg
    10441 agggaacgcg cgagttgccg cggcgcatcc tgcatccgaa gcgcaagacg atcggcctgc
    10501 ggattccaga tcaccgtgtc atccaggcgc tgctcgaggt gctcggcgag ccgctgctga
    10561 cgacgacgct gcagcttccc ggcgacgaat cgccgatgac cgatgcctgg gaaatccgtg
    10621 aacggctcga caagctggtc gatctggttc tcgacggggg ctcctgcggc accgattcga
    10681 cgacggtgat cgacctgact ggccagtcac ccgaggtggt gcgggccggt cgtgggccgc
    10741 tcgagccgct cgggctcgcc tgaaattctg atagagtgaa gtcggtgcgc cgttgacgga
    10801 aatcattcag acgataagtc tggctctatt gccggtgacg ctcgcgatca cgctgcacga
    10861 ggctgcgcac ggttacgcgg cccggtattt tggtgatccg accgcccatc tggcggggcg
    10921 catcacgctt aatccgatca ggcacatcga tcccatcggc acaattgccg tgcccttgct
    10981 gatcctgttg gtatcgggcg gcggaatgct gtttgggtgg gcgaagccag tgccggtcga
    11041 tttcggacgt cttcgcaagc cgaagcgcga catgacttgg gtggcggggg caggcccggc
    11101 ggcgaacctg ctgatggcgg tcttctgggc ggcgatgctc aagctggcct gggcgatgcc
    11161 cgagaatgcc tacacgatgc cgctggcgct gatgagccag atcggcatca gcatcaacgt
    11221 cgtgctgatg gtgctgaatt tgttgccgct gccgccgctc gatggcgggc ggatcgcggt
    11281 gagcctgctc ccgcatgaga tggcctggcg cttcgcgaag ctcgagcgct ggggtttccc
    11341 gatcctgctg gtgctcttgt tcaccggcgt tctcggggcg gtgttgtttc cgctggtgcg
    11401 tctggtgcgc atggcgatcg ccggactttt cggttttccc ggttaagggg tagcaagaaa
    11461 ctatgtacgc agaacgagtg ctgtcgggca tgcgcccgac gggcgccttg catctggggc
    11521 attaccacgg tgtgctgaag aactgggtcc gactgcaggc cgagcatccg tgcctgttct
    11581 tcgtcgccga ctggcatgca ctgaccaccc attacgacga tccggccggc atagaggcca
    11641 gcgtctggga catggtcgtc gattggctcg ccgccggtgt cgatcccggt cagtcgacga
    11701 tcttcatcca gtcgcaggtg cccgagcacg ccgaactgca cctgctgcta tcgatgatga
    11761 cgccgctggg ctggctcgaa cgcgtgccga cctacaagga ccagcaggaa aagctcgaac
    11821 agaaggatct gtcgacctat ggcttcctcg gttacccgct gctgcagtcg gccgacatcc
    11881 tgatctaccg cgccaaccgc gttccggttg gcgaggatca ggtgccgcac atcgagtttt
    11941 cgcgcgagat cgcccgccgc ttcaaccatc tgtacggccg tgagccgggt ttcgaggaca
    12001 aggcgcgcga ggccgtcaag cggctcggct cgaagaaggc gcgctactac aacaatctgc
    12061 gcacccgcta tcaggaacag ggcgatgcct cggcgatcga gcaggcgcat gcgctgctcg
    12121 acgagatcca gcacgtctcg gtcgccgacc gcgagcggct gttcggctac ctcgaaggca
    12181 ccggcaagat gatcctcgtc gaacccgagg cgatgctgac cgcggcgtcg aagatgcccg
    12241 gcctcgacgg gcagaagatg tcgaagtcgt acaacaacac gatcagcctg cgcgaagacc
    12301 ccgagagcgt tcgccagaag gttcgccgca tgcccaccga tccggcacgc gtgcgccgca
    12361 ccgacgtcgg cgaccccgag cgctgcccag tctggcaact gcatcaggtg tattccaacg
    12421 acgaggtgaa gcagtgggtg cgggaaggct gcacttcggc cggcatcggt tgcctcgact
    12481 gcaaggagcc ggtgatcgag ggcatgctcg ccgagctggc gccgatccag gagcgggcgc
    12541 gcgcctatct cgacgatccg accaccgttc gcaacgtcgt cgccgatggc tgcgagcgcg
    12601 cgcgccggct ggcccacgaa acgatgcgcg atgtccgcga gtcgatgggg ctgcactatg
    12661 actgatttga ccgcgatgcg cggcgagttg gcgtgagcgc acgggagatg cctttggaaa
    12721 aggaagtgcg gcgggtcctg gcaagcggcg cgtcgccgca ggacattgag gccgagctgc
    12781 tggcgatgat ccacgatgtc ttcgccaacc gcattccctt caacaaggtg cttggactcg
    12841 acatcatctc ccttgacggc gaccgcccgg tgcttggttt tccgatgcgc gacgagctgg
    12901 tcggcaactt cttccacggc atcctgcacg gcggcgtgac ctcggccgtg ctcgacgtca
    12961 ccggtggcat cgtcgccttc ctcggcctgc agagaaagct cgtcgacctg ccgatcgccg
    13021 agcggctcga gcgcttctcc aagatcggca ccatcgatct gcgaatcgac tacctgcgcc
    13081 caggcaaggg caagtcattt acggcaaccg gctacgcgct gcgtaccggc aacaaggttg
    13141 cggtggcgcg catggaactg cacaacgaca gcaacgactt gattgccgtc ggcaccggcg
    13201 cctatctggt cggctgaacg gacccgcgga gcatgaacga aaccaacagc cagggcgaac
    13261 tgatcgccga agccaccgag ctggggtcgc cggattcggc cgacagcgcc gtggctcaag
    13321 cggcggcgcc tgctgctggg ccggttgcga tgatctacgg cgagccgctg atgcagctgc
    13381 cgctcgatct ttacattccg cccgatgcgt tggcgatcat gctcgagagc ttcgagggtc
    13441 cactcgacct gctgctttac atgatccgca agcagaacgt cgacatcctc gacatcccaa
    13501 tggcctcgct gacccgccag tacctcgaat atgtcgaggc gatgcgcagc cagaacctcg
    13561 aactcgccgc cgaatatctg gtgatggcgg cgatgctgat cgagatcaag tcgcgcatgc
    13621 tgctgccgcg gccgaaggcc gccgagggcg aggaaaccgg cgatccgcgt gccgaactcg
    13681 tgcgccgcct gatcgagtac gagcagatga agctcgccgc gcagaaggtc aacgagctgc
    13741 cgcaggccga ccgcgaattc cagtgggtct cggtgctggt cgagaagtcg gcggtcgagc
    13801 gcctgcccga cgtgaacctc gacgacctgc gcagcgcctg gcttggcatc gtccgccaag
    13861 ccaagctgca tgcccatcac cacgtgcgcc gcgaagaact gtcggtgcgc gagcacatgg
    13921 gcctgatcct gcgccacctc aagagcgccc gctccgacgg ccatggcttt gtcgagttcg
    13981 cgctgctgtt taagcccgag ctgggcgtgc ccggcgtcgt cgtcaatttt ctggcgatgc
    14041 tcgaattggc gcgcgagcat ctgatcgaag tgacgcagtc ggaggccttt gcgcccatct
    14101 acgtgcgttc cgccgaggga gaacctgatg tcatcactgc ctgagaatcc cgaccgcatg
    14161 aagcgcgtgc ttgaagcggc cctgctggcc gcgcagcagc cgatgacgct ggttgagctg
    14221 gcgcgcatgt tcgacgagga cgtgtcggcc gacctgctgc gcaagctgct cgacgaaatc
    14281 cgcgatgcct gggcggaatc ggcgatcgag ctggtgcagc tggcaagcgg ctggcgtttc
    14341 cgtacgcgcg gcgaattcct gccctatctc gaccgcctca accccgacaa gccgccgaag
    14401 tactcgcgcg ctgtgctcga aacgctggcg ataatcgcct acaaacaacc ggtgacccgc
    14461 ggcgacatcg agtcgatccg tggcgtcacg gttggctcac cggtcatcaa gacccttgaa
    14521 gaacgcggct ggatcgacgt cgtcggccac cgcgacaccc cgggacggcc ggctctgttc
    14581 gccaccaccc ggaagtttct cgacgacctc ggcctgcgca gccttactga actgccgccg
    14641 ctggaggaag tcgccccgtt gctggagaac caagatgtcg catgaagccg aagagcgc
//
LOCUS       DATDAA010000077         2027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_494684,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000077 DATDAA010000000
VERSION     DATDAA010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494684"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VIS73_02565"
     CDS             <1..771
                     /locus_tag="VIS73_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY5762067.1"
                     /translation="YRPTPDRPDRAEMSLDTLIPDNPTKPYDIKEAVYKVVDDNEFWE
                     LQPDHAKNIVIGLARMDGHPVGIVANQPLVQAGCLDIKSSIKAGRFVRFCDAFNIPIV
                     TFVDVPGFMPGSAQEYGGIIKHGAKLLFAYAEATVPKITVITRKAYGGAYDVMSSKHL
                     RGDVNLAWPSAEIAVMGPKGAVEIIFRKDLGDQDKIAERTEEYRQKFANPFIAASRGY
                     IDDVIAPHATRQRICRSLAMLREKQLENPWRKHDNIPL"
     gene            792..>2027
                     /locus_tag="VIS73_02570"
     CDS             792..>2027
                     /locus_tag="VIS73_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY5762068.1"
                     /translation="MFDKILVANRGEIACRVLKTARRMGIRTVAVYSDADRDALHVRL
                     ADEAVHIGAAPSTQSYLLGDRIIRACRDTGAQAVHPGYGFLSENAGFCKALAKAGIVF
                     IGPNTRAIEAMGDKITSKKLADKAGVNTIPGYSGVLRDADHAVKEAGRIGYPVMLKAS
                     AGGGGKGMRIARNEAECRDGFMRAANEARSGFGDDRILVEKFIEEPRHIEIQVMADSH
                     GNVLYLNERECSLQRRHQKVIEEAPSPFLDEDTRRRMGEQAVALAKAVDYQSAGTVEF
                     IVDPQRNFYFLEMNTRLQVEHPVTELTTGLDLVELMIRVAAGEKLPLGQDDVGIEGWA
                     IEARVYAEDPFRNFLPSTGRLVYYRPPAEDEHVRVDTGVYEGGEVSMFYDPMIAKLIA
                     HGATRDEALLKLRTALDRFV"
BASE COUNT          395 a          693 c          643 g          296 t
ORIGIN      
        1 taccgcccca cgcccgaccg cccggaccgt gccgagatgt cactggacac gctgattccg
       61 gacaacccga ccaagcccta cgacatcaag gaagccgtct acaaggtggt ggacgacaac
      121 gagttctggg agctgcagcc cgaccacgcg aaaaacatcg tcatcggcct ggcgcggatg
      181 gacggccacc cggtgggcat cgtggccaac cagccgctgg tgcaggccgg ctgcctcgac
      241 atcaagtcct cgatcaaggc cggccgcttc gtgcgcttct gcgacgcctt caacatcccc
      301 atcgtcacct tcgtcgacgt ccccgggttc atgcccggca gcgcccagga atacggcggc
      361 atcatcaagc acggggccaa gctgctgttc gcctacgccg aggccacggt ccccaagatc
      421 acggtgatta cacgcaaggc ctacggcggc gcctacgacg tcatgagctc caagcacctg
      481 cgcggtgacg tgaacctggc ctggcccagc gcggaaattg ccgtcatggg gccgaagggc
      541 gcggtggaga tcatctttcg caaggacctg ggcgaccagg acaagatcgc cgagcgcacc
      601 gaggaatacc ggcagaaatt cgccaacccg ttcatcgccg ccagccgcgg ttacatcgac
      661 gatgtgatcg cgccgcacgc gacgcgtcag cgtatctgcc gctcgctggc gatgctgcgt
      721 gaaaagcaac tggaaaaccc ctggcgcaaa catgacaaca ttccgcttta ggcgcaccgg
      781 agggaccgca ggtgtttgac aagatcctgg ttgcaaaccg cggcgagatc gcctgccgcg
      841 tgctgaaaac ggcgcgcagg atgggcatcc gcacggtcgc cgtctattcc gatgccgacc
      901 gcgacgcgct gcacgtccgc ctggccgacg aagccgtgca catcggcgct gcgccatcga
      961 cgcagagcta cctgctgggg gaccgcatca tccgggcctg ccgcgacacc ggcgcccagg
     1021 cggtgcatcc gggttacggc ttcctgtccg aaaacgccgg gttctgcaag gcgctggcga
     1081 aggcaggcat cgtcttcatc ggccccaaca cccgcgcgat cgaagcgatg ggcgacaaga
     1141 tcacctccaa gaaactggcc gacaaggccg gcgtcaacac catcccgggt tattcggggg
     1201 tgctgcgcga cgccgatcac gcggtgaagg aggccggccg catcggctac ccggtgatgc
     1261 tgaaagccag cgccgggggc ggcggcaagg gcatgcgcat cgcgcgcaac gaggccgagt
     1321 gccgcgacgg tttcatgcgc gcggcgaacg aggcgcgctc gggcttcggc gacgatcgca
     1381 tcctggtcga aaaattcatc gaggagccgc gccacatcga aatccaggtc atggcggaca
     1441 gccacggcaa cgtgctctac ctgaacgagc gcgaatgctc gctgcagcgc cgccaccaga
     1501 aggtcatcga ggaggcgccc tcccccttcc tggacgagga cacgcgccgg cggatgggcg
     1561 aacaggccgt cgccctggca aaagccgtcg actaccagtc ggccggaacg gtcgagttca
     1621 tcgtcgatcc gcagcgcaat ttctatttcc tggagatgaa cacgcgcctg caggtcgagc
     1681 acccggtcac cgagttgacc accgggctcg acctggtcga actgatgatc cgcgtggccg
     1741 ccggcgagaa actgccgctc ggccaggacg acgtcggcat cgagggctgg gccatcgagg
     1801 cgagagttta cgccgaggac cccttccgca atttcctgcc ctccaccggc cgcctggtgt
     1861 attaccgccc gccggccgaa gacgaacacg tccgcgtcga taccggcgtg tacgaggggg
     1921 gcgaggtgtc gatgttctac gacccgatga tcgccaagct catcgcacac ggcgccaccc
     1981 gcgacgaggc gctcctgaag ctgcgcacgg cgctcgaccg cttcgtg
//
LOCUS       DATDAA010000078         7202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_145066,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000078 DATDAA010000000
VERSION     DATDAA010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145066"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /gene="rph"
                     /locus_tag="VIS73_02575"
     CDS             complement(<1..707)
                     /gene="rph"
                     /locus_tag="VIS73_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY5762069.1"
                     /translation="MRHSQRASAELRPLRLTREFTVHAEGSVLIEMGGTRVLCTASVE
                     ESLPPFLRGKGQGWVTAEYGMLPRATHTRSAREAAKGKQSGRTQEIQRLIGRSLRAVV
                     DLKALGERQITLDCDVLQADGGTRCAAITGAFVALSEACNGLVAAGLLTASPVRDHVA
                     AVSVGIVDGAAVLDLDYAEDSEAETDMNVVMNDAGKFVEVQGTAEGHAFDRGEFDALL
                     ALAAKGVGEIIVEQAAA"
     gene            complement(728..1639)
                     /locus_tag="VIS73_02580"
     CDS             complement(728..1639)
                     /locus_tag="VIS73_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEY5762070.1"
                     /translation="MKFTIYQESRQGKRANNEDRIAYCYSRDALLMIVADGMGGHYYG
                     EVASQIAVQYLTSAFQREAQPTLTDPFLFLHKSMSNAHHAILDFSAENKLKDSPRTTC
                     VACIIQNNIAYWAHAGDSRLFHIRKKRVMAQTRDHSRVRLLLEQGMITEQEAATHPDR
                     NKVYSCLGGTTLPEIEFSKKTPLETGDLLVLCTDGVWGVIDGERIAPMLAGSNLMQSV
                     PQMMSQAELEAGPHGDNLSIVAVRWEGNYELPTDQVISTHTLANGEIQTQVPTFGRGA
                     TQKVDLTEDEIEKAIAEIKAAITKFSR"
     gene            complement(1716..2660)
                     /locus_tag="VIS73_02585"
     CDS             complement(1716..2660)
                     /locus_tag="VIS73_02585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5762071.1"
                     /translation="MTQQVNHALPVGYMLDEYEIERQLSLGGFSIVYLARDGHGQPVA
                     IKEYLPNSLALRGENAIVPTISEDHLPAFRYGMKCFFEEGRALAKLSHPNVVRVLNFF
                     RSNETVYMVMEYERGMTLQEYIVKHKGEVRESFIRGVFNRLLNGLREVHANKLLHLDL
                     KPSNIYLRSDSHPVLIDFGAARQTLLADTPMLKPMYTPGFASPEHYKNRENLGPWSDI
                     YSVGASMYACLAAAPPPAADARQEKDNYQAASSRFRGQYSNQLLETIDWCLCLDHLYR
                     PQSVFALQKALTVAHKQPVPKKTWIGGLVSRFKGTAVK"
     gene            2971..3840
                     /locus_tag="VIS73_02590"
     CDS             2971..3840
                     /locus_tag="VIS73_02590"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY5762072.1"
                     /translation="MSAIHSMTGYAACNVSLPGATLGIELRSVNSRFLDIHFRLPDEL
                     RPSETVLREAINGALGRGKVDCRATVTGTSPGLQSAMNPEALASLKTVLGAIGKEFPE
                     VRPAGVGDILRWPGLFGDSGPDFAACNAAMKPLVGQCLTELKACRAREGAQLAAALRE
                     RLAAMRQIITDIAPMIPAAHAAFETKIRQRLLDALGSAEDERIRQEIVIYATRVDVAE
                     EVSRLNAHIDETERVIAKGGTAGKRLDFLMQEFNREANTLGSKSVSGDVSAAAMELKI
                     LIEQMREQVQNIE"
     gene            3855..4457
                     /gene="gmk"
                     /locus_tag="VIS73_02595"
     CDS             3855..4457
                     /gene="gmk"
                     /locus_tag="VIS73_02595"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY5762073.1"
                     /translation="MKGQLFVVSAPSGAGKTTLTRMLLAADDGIRLSVSHTTRGRRPG
                     EEDGRHYHFIDTATFEAMRERGEFLEFAEVHGNYYGTSKAWISERLAAGEDVLLEIDW
                     QGARQVRASFPDAVSIFVMPPSLAELKRRLDTRATDSTEVIARRIAAAEGEMRQVDDF
                     AYAIINEDLERAFGDLQAIIRAARLAVPLQRARRPEVFLK"
     gene            4477..4683
                     /gene="rpoZ"
                     /locus_tag="VIS73_02600"
     CDS             4477..4683
                     /gene="rpoZ"
                     /locus_tag="VIS73_02600"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY5762074.1"
                     /translation="MARITVDDCIKLIPNRFQMTLAATFRARQIAGGSAPLVEADKDK
                     PTVIALREIAGGLVGLEVLNRGQA"
     gene            4717..6912
                     /locus_tag="VIS73_02605"
     CDS             4717..6912
                     /locus_tag="VIS73_02605"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY5762075.1"
                     /translation="MNSAPDFSAAGALPADFSDTAPEVEALPWYEEFTPKLAYLSDDE
                     RRTVDDAFVFAANAHAGQRRVSGEPYISHPIAVAAQVSEWHLDAQALAAALLHDVLED
                     SKVTYADIESRFGKSVADMVDGLSKLDKLEFESYAEAQAENFRKMLLAMSRDLRVILV
                     KLADRRHNMLTLGAIRPEKRQRIARETLDIYAPIAHRLGLNKLCRELQNLAFAQTHPF
                     RYRVIGKAVQAARGSRRELLQKIDDAVRAKLALAGIEAEVSSRQKTEFSIHRKMQEKH
                     LSFSQVLDIFGLRVVVAEPATCYLALGALHSLYKPIPGKFKDYIAIPKVNGYQSLHTA
                     LIGPYGSPIEVQIRSRDMNRVAEHGVASHWLYKSGDESRDAIRFQTHKWLQSLLEMQQ
                     GDADSREFFEHVKVDLFPDEVYVFSPAGQIFELPRGATPVDFAYAVHTDIGHRCIAAR
                     INGTLAPLRSELCNGDRVEIVAAENASPNPAWLAYVRTGRARSKIRQFIKNRQQDEAA
                     HLGERFLDQALRVFGGALSLIGEATWARFVQDVNAKSKADLLVDIGLGRRNPSLDARQ
                     LMVLGQFDVAAPKEALPLQVTGDEGTTLQFASCCHPIPGDPVIGLVSRGRGIVLHTHD
                     CPQLASAGGGPDRRIDVEWDVPEERLFDVTVRCEVRNERGVLARVATAISEAGANIEN
                     VIMDDARRDSITSLQFTIQVHHRIHLAKVMRNIRRVSEVVRVARIRESR"
     gene            6912..>7202
                     /locus_tag="VIS73_02610"
     CDS             6912..>7202
                     /locus_tag="VIS73_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5762076.1"
                     /translation="MRILVVGAGVVGVSSAWYLARAGHSVTVVDRHEAPACETSFANG
                     GQISVSHAEPWANPHTPFAALRWLFRDDAPMLLRPRADRELGAWLVRFLRECL"
BASE COUNT         1277 a         2391 c         2240 g         1294 t
ORIGIN      
        1 atcgccgccg cctgctctac gatgatctcg ccgacgccct tggcggccag cgccaggagc
       61 gcgtcgaact cgccgcgatc gaaagcatgc ccttcggcgg tcccctgcac ctcgacgaat
      121 ttgccggcgt cgttcatgac gacgttcatg tccgtttcgg cctcggaatc ctcggcgtag
      181 tcgaggtcga ggacggccgc accgtcgacg atgccgaccg agaccgcggc gacatggtcg
      241 cgcaccggac tcgccgtcag cagtccggcg gcgaccagtc cgttgcacgc ctccgagagc
      301 gcgacgaagg cgccggtgat cgccgcgcag cgcgtaccgc catcggcctg caacacgtcg
      361 caatcgagcg tgatctgccg ctcgccaagc gccttcagat cgacgacggc gcgcaggctg
      421 cggccgatca ggcgctggat ttcctgggtg cggccgctct gcttgccttt cgccgcctcc
      481 cgcgccgagc gcgtgtgggt cgcgcgcggc agcatgccgt attcggcggt tacccagccc
      541 tggcccttgc cgcgaaggaa aggcggcagc gattcctcga ccgacgcggt gcacaggaca
      601 cgcgtgccgc ccatctcgat cagcaccgaa ccctcggcat ggacggtgaa ctcgcgcgtg
      661 aggcgcaacg ggcgaagctc ggcgctggcg cgctgactgt gacgcattcg gtggtttcct
      721 tgtttcgtta ccgggagaac ttggtgatcg ccgccttgat ttcggcgatc gccttttcga
      781 tttcgtcttc ggtgagatcg accttctgcg tcgcgccgcg accgaaggtc gggacctgcg
      841 tctggatttc gccgttggcc agcgtgtgcg tggagataac ctggtcggtc ggcagttcgt
      901 agttgccttc ccagcgcacc gcaacgatcg acaggttgtc gccatgcggt ccggcctcaa
      961 gctcggcctg actcatcatc tgcggcaccg actgcatcag attcgaaccc gccagcattg
     1021 gcgcgatccg ctccccatcg atcacccccc aaaccccgtc ggtgcacagc acgagcaggt
     1081 cgccggtctc gagcggggtc ttcttcgaaa attcgatctc cggcagcgtc gtgccgccga
     1141 ggcaggaata gaccttgttg cggtcggggt gggtcgccgc ttcctgctcg gtgatcatgc
     1201 cctgctccag cagcagccga acccgcgaat ggtcgcgcgt ctgcgccatg acccgcttct
     1261 tgcggatgtg gaacagccgc gagtcgccgg cgtgcgccca gtaggcgatg ttgttctgga
     1321 tgatgcaggc gacgcaagtg gtgcgcggtg aatccttgag cttgttctcg gccgagaaat
     1381 cgaggatcgc gtggtgcgcg ttgctcatgc tcttgtgcag gaacaggaag ggatcggtca
     1441 gcgtcggctg cgcctcgcgc tggaaggcgc tggtcaggta ctggacggca atctgcgagg
     1501 caacctcgcc gtagtagtgc ccgcccatgc cgtcggcgac gatcatgaga agcgcatcgc
     1561 gcgagtaaca ataggcgatg cggtcctcgt tgttggcccg cttgccctgc ctgctctcct
     1621 ggtaaatcgt gaacttcatc cggctctccc tgccagtcga tcctgccgag taccgaacgc
     1681 ccggcgctgt gcctcaggtc ccatcgcaat aggcttcatt tgacggccgt ccccttgaaa
     1741 cggctgacca agccaccgat ccacgtcttc ttcggcaccg gctgtttgtg cgcaaccgtc
     1801 agcgccttct gcagcgcaaa gacgctctgc ggtcggtaca ggtgatcaag acacaggcac
     1861 cagtcgatcg tctcgagcag ctggttcgag tactgcccgc ggaagcgcga actggctgcc
     1921 tgatagttgt ccttctcctg ccgcgcgtcg gccgccggcg gcggcgctgc ggcgaggcac
     1981 gcgtacatcg acgcgccaac cgagtagata tcgctccagg ggcctaggtt ttcgcggttc
     2041 ttgtagtgct cgggcgaggc gaagcccggc gtgtacatcg gcttcagcat tggcgtgtcg
     2101 gcgagcagtg tctgccgcgc cgcgccgaag tcgatcagca ccggatggct gtccgagcgc
     2161 aggtagatat tggaaggctt gaggtcgaga tgcagcagct tgttggcatg cacttcgcgc
     2221 agaccgttga gcagccgatt gaagacaccg cgaatgaagc tctcgcggac ctcgcctttg
     2281 tgtttgacga tgtactcctg cagcgtcatg ccgcgctcgt actccatcac catgtaaacg
     2341 gtttcgttgg agcggaagaa gttcagcacg cgcacgacgt tggggtgcga gagcttggcc
     2401 agcgcccggc cttcctcgaa gaagcatttc atgccatagc ggaacgccgg caggtgatcc
     2461 tcgctgatgg tcggaacgat ggcgttttcc ccgcgcaagg cgaggctgtt cggcaggtat
     2521 tccttgatcg caaccggctg gccgtggccg tcacgcgcga gatagacgat cgagaacccg
     2581 ccgagcgaca actggcgctc gatctcgtac tcatcgagca tgtagcccac cggcaaggcg
     2641 tggttgactt gctgcgtcat tgcccctcga tcatcgattg acggcgccgg atgacgccat
     2701 cgccccaaca gctgcgaaac aaactcgcgc ggcagccggc aacacggggc ttgggcagcg
     2761 gcgagcgaag cggcagaacc accactcgtc cgacaggcgc aggcaacgat ggtccgtcaa
     2821 gcgcagcaga attcattcgg catcaggcgc cacggctcgg cttctcccct tcgccgatag
     2881 cgcctaagat accggcttcg caattttggt gcctcgaacc gcccctgtaa agcagcggcc
     2941 cggtcgatgc cctactcgca aggacgcgca atgagcgcca ttcacagcat gaccggctat
     3001 gccgcatgca atgtttccct gcccggcgcc actctgggca tcgagcttcg ctcggtgaac
     3061 agccgcttcc tcgacatcca ctttcgcctt cccgacgagt tacggcctag cgaaaccgtg
     3121 ctgcgcgaag cgatcaacgg cgcgcttggg cgcggcaagg tcgattgccg ggctacagtt
     3181 accggaactt cgccgggcct gcagtcggcg atgaaccccg aggcgctggc cagtctcaag
     3241 accgttctcg gcgccatcgg caaggagttc ccggaggtcc gccctgccgg cgtcggcgac
     3301 atcctgcgct ggccaggtct gttcggcgac agcgggcccg acttcgctgc ctgcaacgcg
     3361 gcaatgaagc cgctcgtcgg ccagtgcctg accgaactaa aggcctgccg tgcgcgcgaa
     3421 ggcgcgcagc tggctgcggc gctgcgcgag cgcctcgccg ccatgcggca aatcatcacc
     3481 gacatcgcgc cgatgattcc ggctgcccac gcggcctttg aaacgaagat ccgccagcgc
     3541 ctgctcgatg cgctcggcag tgccgaggac gagcgcatcc gccaggagat cgtcatctac
     3601 gccacgcgtg tcgatgttgc cgaagaggtt tcccgtctca acgcccacat cgacgaaacc
     3661 gaacgcgtca tcgccaaagg cggcacggcc ggcaagcggc tcgacttcct gatgcaggag
     3721 ttcaaccgcg aggcgaatac gctgggctcc aaatcggtat ccggcgacgt ctcggcggca
     3781 gccatggaac tgaagatcct gatcgagcag atgcgcgagc aggtacagaa cattgaatga
     3841 gcgggagcga agccatgaag ggccagctgt tcgtcgtcag cgcgccgtct ggcgcgggca
     3901 agacgacact gacgcgcatg ctgctggcgg ccgacgacgg catccgcctc tcggtctcgc
     3961 acacgacgcg cggtcgccgg ccaggcgagg aggacgggcg ccactaccac ttcatcgaca
     4021 cggcgacctt cgaggcgatg cgcgaacgcg gcgaattcct tgagttcgcc gaagtccacg
     4081 gcaactacta cggcacctcg aaggcatgga tcagcgagcg ccttgccgcc ggcgaggatg
     4141 tgctgctgga aatcgactgg cagggcgcgc gtcaggtccg cgcgtccttt cccgacgcgg
     4201 tgagcatctt cgtgatgccg ccctcgctcg ccgaactgaa acgccgcctc gacacgcgcg
     4261 ccaccgatag cacggaagtc atagcccgtc ggattgccgc agcagaaggg gaaatgcggc
     4321 aagtcgatga ttttgcttat gctattatta acgaggactt ggagcgcgct ttcggcgacc
     4381 tgcaggcgat cattcgtgcc gcaaggctcg ccgttcccct gcaacgcgcc cgtcgccccg
     4441 aggtcttcct gaagtaactg cccaaccgag gttcatatgg cccgcatcac tgtagacgac
     4501 tgcatcaaac tcattcccaa ccggtttcag atgacgctgg ccgccacctt ccgcgcccgc
     4561 cagattgccg gtggcagcgc gccgctggtc gaagctgaca aggacaagcc cacggtcatc
     4621 gccctgcgcg aaatcgccgg cggattggtc ggactggaag tgctcaaccg cggccaggcc
     4681 tgactcccct gccgaaaatc ggggtgagtc cggaccatga actcggctcc cgacttttcg
     4741 gccgctggcg cactgccggc tgatttttcc gataccgcgc cggaagtcga agcgcttccc
     4801 tggtacgagg aattcacccc aaagctcgct tatctcagcg acgacgaacg tcgcacggtt
     4861 gatgacgcct tcgtcttcgc ggccaatgcc cacgctggcc aacggcgggt ctcaggcgaa
     4921 ccgtacatct cgcacccgat cgccgtcgcc gcccaggttt ccgagtggca cctcgacgcg
     4981 caggcgctcg cggcagccct gctgcacgac gtactcgaag actcgaaggt cacctacgcc
     5041 gacatcgaga gccgattcgg aaagtcggtc gccgatatgg tcgatggcct ctccaagctc
     5101 gacaagctcg agttcgagag ctacgccgag gcgcaggccg aaaacttccg caagatgctg
     5161 ctggcgatgt cacgcgatct gcgggtcatc ctcgtcaagc tcgccgatcg acggcacaac
     5221 atgctcacgc tcggggcgat ccgccccgaa aagcggcagc gaatcgctcg cgagacgctc
     5281 gacatctacg caccgatcgc gcaccggctt ggcctcaaca agctctgccg ggaactgcag
     5341 aacctcgcct ttgcgcagac ccatccgttt cgctatcgcg tcatcggcaa ggcggtccag
     5401 gcagcgcgcg gcagccgccg cgaactgctg cagaaaatcg acgacgcggt tcgcgccaaa
     5461 ctcgcgctgg cgggcattga ggctgaggtc agcagccggc aaaagaccga gttcagcatc
     5521 caccgcaaga tgcaggaaaa gcacctgtcg ttctcgcagg tactcgacat cttcggcctg
     5581 cgcgtcgtcg tcgccgagcc ggcgacctgc tacctcgcgc tgggcgcact gcactcgctc
     5641 tacaaaccga ttcccggcaa gttcaaggac tacattgcga tccccaaggt caacggttat
     5701 cagtcgctgc atacggcgtt gatcggcccc tacggttcgc cgattgaggt gcagatccgg
     5761 tcgcgggata tgaaccgcgt tgccgagcat ggggtggcgt cgcactggct ctacaagtcg
     5821 ggcgacgagt cgcgcgacgc aatccgcttc cagactcata aatggctaca gtcgctgctc
     5881 gagatgcagc agggcgatgc cgattcgcgc gaattcttcg aacacgtcaa ggtcgacctt
     5941 ttccctgacg aggtctacgt gttctcgccg gccgggcaga tcttcgagtt gcctcgtggc
     6001 gcaacgcccg tcgatttcgc ctatgccgtg cataccgaca tcggccaccg ctgcatcgcg
     6061 gcgcgcatca atggcaccct agcaccgctg cgcagcgagc tatgcaacgg tgatcgcgtc
     6121 gagatcgtcg ccgccgagaa tgccagcccg aatccggcct ggcttgccta cgtcaggacc
     6181 ggccgcgccc gcagcaagat ccggcagttc atcaagaatc gccagcagga cgaagctgcg
     6241 caccttggcg agcgcttcct tgatcaggca ctgcgcgtct ttggcggcgc actgtcgctg
     6301 atcggcgagg cgacctgggc gcgattcgtc caggacgtca acgccaagtc gaaggccgat
     6361 ctgctcgtcg atatcggcct tgggcgccga aatccctcgc tcgacgcgcg ccagctcatg
     6421 gtgctgggcc agttcgatgt tgccgcgccc aaagaggcat taccgctgca ggttaccggc
     6481 gatgaaggca cgaccctgca atttgccagc tgctgccacc cgattccggg agacccggtc
     6541 atcggtctgg tcagccgggg gcgcggcatc gtgctgcaca cacacgactg cccgcagctc
     6601 gccagcgccg gcggtggtcc cgatcgccgg atcgacgtcg aatgggacgt tccagaggag
     6661 cgactgtttg acgttacggt ccgctgcgag gtccgtaacg agcgcggtgt gctggcccgc
     6721 gtggcgaccg caatatctga ggccggcgcc aacattgaga atgtcatcat ggacgacgcg
     6781 cgacgtgact cgatcacctc gctgcaattc accattcagg tccatcaccg gatccatctg
     6841 gcgaaggtaa tgcgcaacat ccgccgcgtt tccgaagtcg ttcgcgtcgc ccgtatccgg
     6901 gaatcgcgct gatgcggatt cttgtcgtcg gcgccggcgt ggtcggcgtc agcagcgcat
     6961 ggtatctcgc ccgcgcgggc cattcggtca ccgtcgtcga ccggcatgag gcgccggcct
     7021 gcgagacgag cttcgccaac ggcggtcaga tatcagtgtc gcatgcggag ccttgggcca
     7081 acccgcacac gccattcgcc gcactgcgct ggctgtttcg cgatgacgca ccgatgctgc
     7141 tgcggccacg cgccgaccgc gaactgggcg cctggctggt ccgcttccta cgcgaatgcc
     7201 tg
//
LOCUS       DATDAA010000079         2263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_250330,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000079 DATDAA010000000
VERSION     DATDAA010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250330"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VIS73_02615"
     CDS             <1..1033
                     /locus_tag="VIS73_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY5762077.1"
                     /translation="LDLAGDSIDIARYMAPAGEDAAAEETSGGPVEIPVDLIRALNAR
                     GNLSITSASLGKIPFENVTLGVNSANGKLRIHPIAADFFGGGYRGDVKIDASGKQPSI
                     AVDENIVNVSLRPMMQAMYEVDNVSGIINGTFRLGGSGADVDAIRRDLDGTLSIELID
                     GAWEGTDVWYELRKARSLVRGEPAPAAPATPRTQFSSMKASGQVTDGVMRNDDFFAEL
                     PFMRVSGKGTVNFVEATVDYTVTGRFLEKPEFMTQVSDDELDDFTKAVIPFRITGPIA
                     DPAIRPDVEEMLKDRAEQEAKKALRDKLFGDDREPPAEGEEPQEEKDLEDQLKDEAKK
                     RLKDLLGGD"
     gene            1036..1596
                     /locus_tag="VIS73_02620"
     CDS             1036..1596
                     /locus_tag="VIS73_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5762078.1"
                     /translation="MRALFLLVIALPVCAGAATLRAVNVDHVDGIYIMHSEVWFDVSI
                     EQIYGLLLDWDQSTKFSSVIVESRNLDPGPDGRARYYSHTRGCLWFFCKSFERYGYVE
                     HQPFRFIKASVDPEKSDFEVSDESWEFREEADGTVVTYAFRMKPKFFIPPLIGPAIMK
                     NKLRNSGINAIERIEALARDYAPYED"
     gene            1586..>2263
                     /gene="mutY"
                     /locus_tag="VIS73_02625"
     CDS             1586..>2263
                     /gene="mutY"
                     /locus_tag="VIS73_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY5762079.1"
                     /translation="MKTDFATRVLHWYDQHGRTDLPWQDTGDPYRVWVSEIMLQQTQV
                     QTVIPYYRRFMHRFPDLASLAGARLDDVLQHWSGLGYYARARNLHYAAEIIRDRHDGR
                     FPETIEAVCELPGIGRSTAGAILALTFDQRHAILDGNVKRVLARHAAVDGWPGKTAVA
                     RKLWELAEARTPPERVGAYTQAMMDLGATLCTRSRPACAMCPVQGDCRALARGEVERF
                     PGRKSKKE"
BASE COUNT          450 a          687 c          758 g          368 t
ORIGIN      
        1 actggatctc gcgggcgaca gcatcgacat cgcccgttat atggcgcccg cgggcgagga
       61 cgccgcggcg gaagagactt cgggcggacc ggtcgagatc cccgtcgacc tcatccgggc
      121 gctgaatgcg cgcggcaacc tgagtattac cagcgcgagc ctcggcaaga taccgttcga
      181 gaacgtgacg ctcggcgtga actccgcaaa cggcaagttg cgcattcacc cgatcgcggc
      241 cgacttcttc ggcggcggct atcgcggcga cgtgaagatc gatgcgtccg gaaaacagcc
      301 gtcgattgcc gtcgacgaga atatcgtgaa cgtcagcctg cgcccgatga tgcaagcgat
      361 gtacgaggtc gacaacgttt ccggcatcat caacggcacg ttcaggctcg gcggcagtgg
      421 cgctgacgtg gatgcgatac gccgcgacct cgatggcacc ctgtcgatcg agctcatcga
      481 cggcgcctgg gagggcacgg acgtctggta cgagctacgc aaggcgcgct cgctcgtgag
      541 aggcgaaccg gcgccggcgg cgccggcgac gccacgcacg caatttagct cgatgaaggc
      601 cagcggccag gtcaccgacg gcgtgatgcg gaacgacgat ttcttcgccg agctgccgtt
      661 catgcgggtg agcgggaagg gcaccgtgaa tttcgtggaa gcgaccgtgg actatacggt
      721 gaccggtcgc ttcctcgaaa agcccgaatt catgacgcag gtcagcgatg acgaactgga
      781 tgatttcacg aaggcggtga tcccgttccg tatcaccggg ccgatcgccg atccggccat
      841 aaggccggac gtcgaggaga tgctcaagga tcgggcggag caggaagcga agaaggcgct
      901 cagggacaag cttttcgggg atgacagaga gccgccggcc gagggcgaag aaccgcagga
      961 agagaaggat ctcgaggatc agctcaaaga cgaagccaag aagcgcctga aggatctgct
     1021 cggcggcgac tgaccatgcg ggcactgttc cttctcgtca ttgccctccc ggtatgcgcc
     1081 ggcgcggcta cgttgcgcgc ggtgaacgtg gaccacgtgg acggcatcta catcatgcat
     1141 tccgaagtgt ggttcgatgt cagcatcgag cagatctacg gccttttact cgactgggac
     1201 cagtcgacga aattctcgag cgtcatcgtg gagtcgcgga acctcgaccc agggccggac
     1261 gggcgggcgc gttactacag ccatacccgc ggctgcctgt ggttcttctg caagtccttc
     1321 gagcgctacg gttacgtgga gcaccagccc ttcaggttta tcaaggcaag cgtggacccg
     1381 gagaaaagcg acttcgaggt cagcgacgaa agctgggagt tccgggaaga ggctgacggt
     1441 acggtcgtca cctacgcttt cagaatgaag cccaagttct ttatcccgcc gctcatcggc
     1501 cccgccatca tgaagaacaa gctcaggaac agcggcatca acgcgatcga acgcatcgag
     1561 gctctcgcca gagattacgc gccctatgaa gactgatttc gcgacgcgcg tgctgcactg
     1621 gtacgaccag cacggccgca cggacctgcc gtggcaagac accggcgacc cctatcgtgt
     1681 ctgggtctcg gagatcatgc tgcagcagac ccaggtgcag acggtgatcc catactaccg
     1741 gcgcttcatg caccggttcc ctgacttggc cagtctcgcc ggtgcgcggc tggacgacgt
     1801 gctgcagcac tggtccggtc tcggttacta cgcgcgggcg cgcaacctgc attacgcggc
     1861 ggagatcatc cgtgaccggc acgacggccg tttcccggag accatcgaag cggtttgcga
     1921 actgccggga atcggccggt ccacggcagg cgcgatactc gcgctcacct tcgaccagcg
     1981 ccacgcgatc ctggacggca acgtgaagcg agtgctggcg cggcacgcgg cggtcgacgg
     2041 ctggcccggc aagacggcgg tggcgcggaa gctctgggag ctggcggagg cacgcacgcc
     2101 gcctgaacgc gtcggcgcct atacgcaggc gatgatggat ctcggcgcga cgctgtgtac
     2161 gcgctcgaga ccggcctgcg cgatgtgccc ggtgcaaggc gattgcaggg ccctggcgcg
     2221 aggcgaggtg gagcgtttcc cgggccgcaa gtcgaaaaag gag
//
LOCUS       DATDAA010000080         4473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_355391,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000080 DATDAA010000000
VERSION     DATDAA010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355391"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VIS73_02630"
     CDS             complement(<1..959)
                     /locus_tag="VIS73_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY5762080.1"
                     /translation="MSHLLNPAQREAIRYLDGPLLVLAGAGSGKTRVITQKIAYLIEE
                     CAMPPDTIAAITFTNKAAREMDERVKTLLAGRPTKGLTISTFHSLGMRLLRAEAKAAG
                     YKPGFSIFDSDDSYAIVADLSGSVDKKTIRRLQATISNWKNAGLTPDQAAAEAVSEAD
                     GLAARAYRNYDATLKSYQAVDFDDLIRLPLQLLEENAEVRERWQNRLRYLLVDEYQDT
                     NGLQYRMLKALAGPRAQFTAVGDDDQAIYAWRGADVENLKHLTQDFPQLKVVKLEQNY
                     RSTLRILEAANTLIANNPKLFEKRLWSEHGQGDPITLTACKDA"
     gene            complement(1110..1370)
                     /locus_tag="VIS73_02635"
     CDS             complement(1110..1370)
                     /locus_tag="VIS73_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762081.1"
                     /translation="MFVTELVRNIAWSLRRSRRQLPNAESVLPADAASPVAPTRGERR
                     RNAAQESLEDRVRQPADRPASEAPEVETPGNAGGQPHIDIKV"
     gene            1938..2795
                     /locus_tag="VIS73_02640"
     CDS             1938..2795
                     /locus_tag="VIS73_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY5762082.1"
                     /translation="MSLSPNKTDRPAILAKLAADLKRGDVSFPTGAQIALKLLRTLED
                     PTLHVDQAVRLIQAEPLIAAKAVATANSVAYNPARREVSDVRAAVARLGFRTLRSLTA
                     GVVAKQMAGRCEGRAAEMAEQLWEHTANVGAIASVLARRLTGQDAETALFVGLVHEIG
                     GFYLISRTKDFPDILDDDAQDWPESGEAELAMAVLSVIGAPNAVLEAIAAYWHGQPAM
                     PPRTLGDTLLLADELSPVRSPLAPGRSKFRHGDAAGAVYFLEGEASLQAIIDDSAEVV
                     ASLVSVLKA"
     gene            complement(2828..3766)
                     /gene="lipA"
                     /locus_tag="VIS73_02645"
     CDS             complement(2828..3766)
                     /gene="lipA"
                     /locus_tag="VIS73_02645"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY5762083.1"
                     /translation="MSKIGEKQKGEAKTARIPIKIVPAERLRKPDWIRVKAGNEGGRF
                     GEIKKILRQQKLHTVCEEASCPNIGECFGHGTATFMIMGDICTRRCPFCDVGHGKPLP
                     LDASEPAHLGETVAALKLKYVVVTSVDRDDLRDGGAAHFVDCIRAVRDTSPATRIEVL
                     VPDFRGRLDIALEIFDQALPDVMNHNLETVPRLYRQARPGSDYAHSLKLLQDFKARHP
                     AVPTKSGLMVGLGETDEEILEVMRDLRAHGVDMLTVGQYLQPSNGHLPVLRYVHPDVF
                     RRFESEGLAMGFSNVASGAMVRSSYHADLQAKEVVA"
     gene            complement(3763..4416)
                     /gene="lipB"
                     /locus_tag="VIS73_02650"
     CDS             complement(3763..4416)
                     /gene="lipB"
                     /locus_tag="VIS73_02650"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY5762084.1"
                     /translation="MAAAPAAPGPGLLVRDLGRADYLPTWQAMIAFTAARDETTPDEL
                     WCVEHPPVFTLGQAGKPEHVLRDIGVPVVKIDRGGQVTYHGPGQAVVYLLLDLRRRKL
                     KVGTLVARIEAAVIELLAGHGVEAQRRKGAPGVYLGAGGEGAKIASLGLKVRNGCCYH
                     GVSLNVDLDLAPFAAINPCGYPELAVARTCDLGIPLTTAEAGHRLAEALTRQLEGRP"
BASE COUNT          716 a         1400 c         1535 g          822 t
ORIGIN      
        1 tcggcgtcct tgcaggcggt tagtgtgatc ggatcgccct ggccgtgttc ggaccaaagt
       61 cgcttttcaa acagcttggg attgttggcg atcagcgtgt tggccgcctc aaggatgcgc
      121 agcgtcgaac ggtagttctg ctcgagcttg accaccttga gctgcgggaa gtcctgcgtc
      181 aggtgcttga ggttctcgac atcggcgccg cgccaggcgt agatcgcctg gtcgtcgtcg
      241 cccacggccg tgaactgggc gcgcgggccg gccagcgctt tgagcatgcg gtactgcagg
      301 ccgttggtgt cctggtactc gtcaactagc aggtagcgca gccggttctg ccagcgctcg
      361 cggacttcgg cgttttcttc gagcagttgc agcggcagtc gaatcagatc gtcgaaatcg
      421 acggcctggt agctcttcag tgtcgcgtcg tagttgcggt aggcgcgcgc ggccaggccg
      481 tcggcctcgc tcaccgcctc ggcggcggcc tggtcgggcg tcaggccggc gttcttccag
      541 ttggaaatgg ttgcctgcag ccggcgaatc gtcttcttgt cgacactgcc cgagaggtcg
      601 gcgacgatcg catagctatc gtcggaatca aagatcgaaa agcccggctt gtagccggct
      661 gccttggctt cggcgcgcaa caggcgcatc cccagcgaat ggaaggtcga gatcgtcagg
      721 cccttggtcg gccggccggc gagcagcgtc ttgacgcgct cgtccatctc gcgcgccgcc
      781 ttgttggtga aggtgattgc ggcgatcgtg tccggcggca tcgcgcattc ctcgatcagg
      841 taggcgatct tctgggtgat gacgcgggtc ttgcccgaac cggcgccggc gaggaccagc
      901 agcgggccgt cgaggtagcg gatcgcctcg cgctgggcgg ggttgagtag gtgggacatg
      961 cgggcagatg gcctgagtcg atgcagaaaa agaagacgcc ccggactgcg gggcgtcgct
     1021 ggtctgcatt ttaaccgagg gcaggcgtcg cgtaccggga gcgtggaggc ggccgcgcgt
     1081 acgtcagtgc ctgcggatac caggctcgct cagaccttga tgtcgatgtg cggctggccg
     1141 ccagcgttgc cgggtgtctc gacctcgggc gcctcgctcg ccgggcggtc ggcgggttgc
     1201 cggacccgat cttcgagtga ctcctgcgcg gcgttgcggc ggcgttcgcc tcgcgtcggc
     1261 gccaccggcg acgcggcatc ggccggcagc accgactcgg cgttgggcaa ctgccgacgt
     1321 gatcggcgca gcgaccaagc gatgttgcgg accagttcgg tgacgaacat tcagacctcc
     1381 ttcaggcgaa cctgttgtcc tttcgaccgc aggcatactg ccttggtgcc cttgctatcg
     1441 gcatcggcgg gtcagccttg aatcgaggct gggggattgc aagccgtttt tcggcgaggg
     1501 cgcgcgttac gtcggcgcag ttgacggccg ttatctccca gaagtcctca tcgatccgcg
     1561 ctgtgagtga gcgcaaacct gcttgtaagg cactgcgaat aggagaaaga cgcctataaa
     1621 gtctccagcg aaagccggtg ctgagcctgg aggcgccatc gccagcattt atgaggggag
     1681 ttttcgcacg aaagcgccaa catcgcgcgt cgcagcagac gagcaatccg cgagtcggga
     1741 tttttccgtt gcacccgcac cacgatgcct tccatttgct gttaatgatc ggttcccgcc
     1801 gagctggctg ccacgccagc gggttcaggg cgctcttccc ccgagttgca tggtcgaacg
     1861 aagtaacatg ccggctttgc aatatccttg tcgccagcga gtaccggact ccgccggaac
     1921 ctctagacga cacgaacatg agcctatccc cgaataagac cgatcggccg gcgatcctgg
     1981 ccaagttggc cgctgacctc aagcgcggcg acgtttcgtt tcccaccggc gcgcagattg
     2041 ccctgaaact gctgcgcacg ctcgaggacc cgacgctgca tgtcgatcag gcggtccggt
     2101 tgattcaggc agagccgctg atcgctgcca aggcggtggc gacggccaat tcggttgcct
     2161 acaacccggc acgccgcgag gtttcggatg tccgggcggc agtcgcgagg ctcggctttc
     2221 gcacgctgcg cagcctgacc gcgggcgtgg tcgccaagca gatggctggc cgttgcgagg
     2281 gccgggcggc ggagatggcc gagcagctct gggaacacac ggcgaatgtc ggcgcgattg
     2341 ccagcgtgct ggcccgacga ctgaccgggc aggatgcgga aaccgcgctg ttcgttgggc
     2401 tggttcacga gatcggtggc ttctacctga tttcgcgcac caaggacttc ccggacatcc
     2461 tcgacgacga cgcgcaggac tggccggaaa gtggcgaggc cgaactggcg atggcggtac
     2521 tcagcgtgat cggcgcgccc aatgccgtgc tcgaggcgat tgccgcctac tggcacggcc
     2581 agccggcgat gccgccaagg acgctcggtg acacgttgct gctcgccgac gagctctcgc
     2641 cggttcgctc gccgctggca cccggacgca gcaagtttcg ccacggcgac gccgcggggg
     2701 cggtctactt cctcgaaggc gaggcatcgc tgcaggcaat catcgacgac tcggccgagg
     2761 tcgtcgcctc gctggtctcg gtgcttaagg cctagcccac cgcagcgaag ccggcgctgc
     2821 cgggccctca ggcgacgact tccttcgcct gcaggtcggc gtggtagctc gatcgcacca
     2881 tcgcgccgct ggcgacattc gagaatccca tcgctagccc ttcggactcg aagcggcgga
     2941 agacgtcggg atgcacgtag cgcaataccg gcaggtggcc gttagagggc tgcaggtatt
     3001 gaccgacggt gagcatgtcg acgccgtggg cgcgcaggtc gcgcatgact tcgaggattt
     3061 cctcgtcggt ttcacccagt ccgaccatca gccccgactt ggtcggcacc gcggggtggc
     3121 gcgccttgaa atcctgcagc agtttgagcg agtgcgcgta atccgagccc gggcgggcct
     3181 gccggtagag gcgcggcacc gtttcaaggt tgtggttcat cacatcaggc agcgcctggt
     3241 cgaagatctc cagcgcaatg tcgagccggc cgcggaagtc ggggaccagc acttcgattc
     3301 gggtcgccgg cgaggtgtcg cgaacggcgc ggatacagtc gacgaaatgc gccgcgccgc
     3361 catcgcgaag gtcgtcgcga tcgacactgg tgacgaccac gtacttgagc ttgagcgccg
     3421 cgacggtctc gccgaggtgg gccggctcgc tggcatcgag tggcagcggc ttgccgtggc
     3481 cgacatcgca gaaggggcag cgacgggtgc agatgtcgcc catgatcatg aaagtggcgg
     3541 tgccgtgacc aaagcactcg ccgatgttgg ggcacgaggc ctcctcgcac accgtatgca
     3601 gcttctgctg gcgcaggatc ttcttgattt cgccaaagcg tccgccctcg ttgccggcct
     3661 tgacgcgaat ccagtcgggc ttgcgcaggc gctcggcggg aacgatcttg atcggaatgc
     3721 gcgcggtctt cgcttcgccc ttttgctttt cgccaatctt gctcatggtc tgccttcgag
     3781 ttgtcgggtc agcgcctcgg cgagccggtg gccggcttcg gcggtggtca gcgggatgcc
     3841 gaggtcgcag gtacgcgcga cggcgagttc ggggtagccg cagggattga tcgcggcaaa
     3901 cggcgccagg tcgagatcga cgttcagtga aacgccgtgg tagcagcagc cgttcctgac
     3961 cttcaatccg agcgatgcga tcttcgcgcc ctcgcctccg gcgcccagat aaacgcccgg
     4021 agcgcccttg cgtcgctggg cttcgacgcc gtgaccggcg agaagttcga tcacggctgc
     4081 ctcgatgcgc gcaaccagcg tgccgacctt gagcttgcgc cgccgcaggt cgagcagcag
     4141 gtagacgacc gcctggcctg gcccgtgata ggtcacctga ccgccgcggt cgatcttgac
     4201 cactggcacg ccgatatcgc gcagcacgtg ttcgggtttg ccggcctgac cgagcgtgaa
     4261 taccggcggg tgctcgacgc accaaagctc gtcgggcgtc gtttcgtcgc gggcagcggt
     4321 aaaggcaatc atcgcctgcc aggtgggcag gtaatcggcg cggcccagat cgcgaaccag
     4381 cagtcccggg cccggcgccg ccggtgcggc ggccatcacg gcagcgctcg ccggttcggt
     4441 cattgcctca gaggacgacc ttgaccaagg ggt
//
LOCUS       DATDAA010000081         4942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_5894,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000081 DATDAA010000000
VERSION     DATDAA010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5894"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VIS73_02655"
     CDS             complement(<1..900)
                     /locus_tag="VIS73_02655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5762085.1"
                     /translation="MASNPSSDRLAAGKNPAHRVDPLDPTRMTSLKHWGEPPEQPGSD
                     MDQRATVECGWGRVIFGHTFASPAEIAEELRDEAPERRDIALYVRDPHVVVAMAPQHL
                     FIDPSHTYRLDLGTLEWCTTGHELWRVRPVAGQDDTAIDRIYRSRRMVPATGFYSDRS
                     RFGKGVEVLVAEDHASGAILGAVTGIDHVRAFGDVDLGSSLWSLAVDPQCPHPGVGEG
                     LVRALASGFAARGRAFMDLSVMYDKTDAIGLYEKLGFVRVPVYCVKNKNPINEWLFIG
                     PDPAGELNVYARILIEEARRRGTM"
     gene            complement(912..2684)
                     /locus_tag="VIS73_02660"
     CDS             complement(912..2684)
                     /locus_tag="VIS73_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="HEY5762086.1"
                     /translation="MCGFSGEVRFDQQAADIVAVQKMSDVLDPRGPDARGSYSQGSVA
                     FAHRRLKIIDLSEHAQQPMVDPALGLAIVFNGCIYNYRHLRLELEALGYRFFSHGDTE
                     TLLKAWHAWGPDCLYKLHGMFAFAIHERDSGRVILGRDRFGIKPLYLSRVGQRLRFAS
                     SLPAILAAGEVDTMLDPVALHHYLSWHAVVPPPQTILQGVRKLPPATLRIIETDGRET
                     DQTYWSLTVDTDPALARLPQTDRNELVHEALRKAVSRRMVADVPVGVLLSGGVDSSLI
                     VGLLAEAGQHGLETFSIGFESAGGEKGDEFEYSDIVARHFDTRHHKIPIATSELVATL
                     PEAIHAMAEPMVSYDNIGFYLLSREVSRHLKVVQSGQGADEVFGGYHWYPPLAASTDP
                     AADYARVFFDRDHAEFQRVVSPEFHGEDHSRIYVERLFAAKHGRSGLDKALHIDTTVM
                     LVDDPVKRVDSQTMAWGLEARVPFLDHELVELAGRLPAEDKIGDGGKAVLKAIARQVV
                     PPAVVDRPKGYFPVPILKHLGGPTLAMVRDALESRAARQRGVFQRDYVDMLLADPVAH
                     MTPLRGSKLWQLGLLELWLQSHGV"
     gene            2720..3301
                     /locus_tag="VIS73_02665"
     CDS             2720..3301
                     /locus_tag="VIS73_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5762087.1"
                     /translation="MQLSHRQKEQSKMARVKQLNTKQKPKIPPVDLDPLSGNWPQLLV
                     ALRQLAQAGELHSRHLISACGLTVPQTLVLRAIDGLEQATAGRISQLVSLSPPTVTAI
                     LNRLEERQLVQRDRGASDRRLVILSLTSRGREVLATAPSLLPPAFMARFAALPLWEQT
                     LLLSSLQRLAEMMGEGAPAALDAVADAGTSVAG"
     gene            complement(3428..3934)
                     /gene="rraA"
                     /locus_tag="VIS73_02670"
     CDS             complement(3428..3934)
                     /gene="rraA"
                     /locus_tag="VIS73_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HEY5762088.1"
                     /translation="MSSKPAFATCDLCDEFDGKPEYGLRVVEPMFRTYGGVPAFAGPI
                     ATLKVFEDNSLVRERLGEPGEGRVLVVDGGGSRRCALVGDQLGELGVKNGWAGIVVYG
                     CIRDSAVIAKQAIGVRALDTHPRKSVKAGVGQRDVVVTFGGVSFAPGEWLYADADGIL
                     VANRALVA"
     gene            complement(3945..4892)
                     /gene="secF"
                     /locus_tag="VIS73_02675"
     CDS             complement(3945..4892)
                     /gene="secF"
                     /locus_tag="VIS73_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY5762089.1"
                     /translation="MEFFRIRNDIPFMRHALVFNIISLVTFALAVFFLTMRGLHLSVE
                     FTGGTLVEARYSESADLDSVRGALSQAGYTDMAVQHFGSTNDVLIRLPLRTEQSSAHV
                     GDSVMAALSQASPDVTLSRVEFVGPQVGRELIENGALALLLVIIGIVVYLAFRFEWRF
                     SVSAIVANLHDVIIILGFFAFFQWEFSLPVLAAVLAVLGYSVNESVVVFDRVRETFRK
                     TRGLTTPQVLDHAITRTISRTVITHGSTQIMVLSMLIFGGEALHYFAVALTIGILFGI
                     YSSVLVASPLVMWLGVSREQFIKPPKEKPEGVTDDGACV"
BASE COUNT          883 a         1514 c         1724 g          821 t
ORIGIN      
        1 catggtgccg cgccggcgcg cttcctcgat gagaattcgc gcgtagacgt tgagttcgcc
       61 ggcggggtcg ggtccgatga acagccactc gttgatcgga ttcttgtttt tgacgcagta
      121 caccggcacg cggacgaaac cgagtttctc gtagagccct attgcgtcgg tcttgtcgta
      181 catcaccgag aggtccatga aggcgcggcc ccgcgctgcg aagccactgg ccagcgcacg
      241 caccaatcct tcgccgacgc cggggtgcgg gcactgcgga tcgacggcga gcgaccacag
      301 gctgctgccg aggtcgacgt cgccgaaggc gcgaacgtgg tcgatgccgg tgacggcgcc
      361 caagatggcg ccgctggcgt gatcctcagc gactaggacc tcgacgccct tgccgaaacg
      421 cgagcgatca ctgtagaagc cggtcgccgg caccatgcgc cggctgcggt agatgcggtc
      481 gatggccgtg tcgtcttggc cagccaccgg gcgtacccgc caaagctcgt ggccggtcgt
      541 gcaccactcg agagttccaa ggtctagccg gtaggtgtgc gagggatcga tgaataggtg
      601 ctgcggcgcc atcgcgacca ccacgtgtgg gtcgcggacg tagagcgcga tgtcgcggcg
      661 ctcgggcgct tcgtcgcgga gctcttcggc gatttccgct ggcgaggcga aggtatggcc
      721 aaatatgacg cgaccccacc cacattcgac ggtcgcgcgc tggtccatgt cgctgccggg
      781 ctgctcgggc ggctcgcccc agtgcttgag ggaggtcatg cgtgtggggt cgagggggtc
      841 aacgcggtgc gccgggttct tgccggcggc aagcctatcg gaactggggt tggaggccat
      901 gctcttctgc ctcagacgcc atgcgactgc agccacagct cgagcagccc gagctgccag
      961 agtttggagc cacgcagcgg cgtcatgtgt gctaccgggt cggcgagcag catgtcgacg
     1021 tagtcgcgct ggaagacgcc gcgttggcgc gccgcacgtg attcgagcgc atcgcggacc
     1081 atcgccagcg tcgggccgcc gagatgcttg agtatgggga ccgggaagta gcccttgggg
     1141 cggtcgacga ctgcgggcgg gaccacctgc cgggcgatag ccttgagcac ggccttgccg
     1201 ccgtcgccga tcttgtcctc ggccggcagg cggccggcaa gttcgaccag ttcgtgatcg
     1261 agaaagggga cgcgggcctc gaggccccag gccatggtct gactgtcgac gcgcttcacc
     1321 gggtcgtcaa ccagcatcac cgtcgtatcg atgtgcaggg ccttgtcgag gccgctgcgg
     1381 ccatgcttgg cggcgaatag ccgctcgaca tagatgcggc tgtggtcttc gccgtggaac
     1441 tcgggcgaga cgacgcgctg gaactcggcg tggtcgcggt cgaagaagac ccgcgcgtag
     1501 tcggcggcgg ggtcggtgct cgcggccagc ggcggatacc agtggtagcc gccaaacacc
     1561 tcgtcggcgc cctgaccgct ctgcaccacc ttgaggtggc gcgagacctc gcgcgagagt
     1621 agatagaaac cgatgttgtc gtagctgacc atcggctcgg ccatcgcgtg gatcgcctcg
     1681 gggagcgtcg cgacgagctc gctggtggcg atcgggatct tgtgatgacg cgtgtcgaaa
     1741 tgccgggcga cgatgtcgga gtactcgaac tcgtcgccct tttcgccgcc cgcggactcg
     1801 aagccgatcg agaaggtctc aaggccgtgc tgcccggctt cggcgagcag gccgacgatc
     1861 aggctcgaat cgacgccgcc cgaaagcagc acgccgaccg gaacgtcggc gaccatgcga
     1921 cgcgacacgg ccttgcgcag cgcctcgtgg accagctcat tgcgatcggt ctgtggcagg
     1981 cgggcgaggg ccgggtcggt atcgacggtg agcgaccagt aggtctggtc ggtttcccgt
     2041 ccgtcggttt cgatgatgcg cagcgtcgcc ggcggcagct tgcgcacgcc ctggagaatc
     2101 gtctgcggcg gcggcactac cgcatgccag ctcagatagt ggtgcagcgc gaccgggtcg
     2161 agcatcgtgt cgacctcgcc ggccgcgaga atcgctggca ggctcgaggc gaagcgcaaa
     2221 cgctggccga cgcgcgacag gtagagcggc ttgatgccga agcggtcacg gccgaggatg
     2281 acgcggccgc tgtcgcgctc gtggatcgcg aaggcgaaca tgccgtgcag cttgtatagg
     2341 cagtccggcc cccaggcgtg ccaggccttg agcagtgttt cggtgtcgcc gtgggaaaag
     2401 aagcggtagc cgagcgcctc gagttcaagg cgcaggtgac ggtagttgta gatacacccg
     2461 ttgaagacga tcgccaggcc cagcgccggg tcgaccatcg gctgctgggc gtgctccgaa
     2521 aggtcgatga ttttcagacg gcggtgggcg aaggcgacgc ttccctgcga gtagctgcct
     2581 cgcgcatcgg gcccgcgcgg gtcgagcacg tccgacatct tttgtacagc aacgatatct
     2641 gctgcctgct ggtcgaagcg gacctctccg gaaaaaccgc acataggcct aatattatgg
     2701 ttttgcgtgt aaattcagcg tgcaattatc gcatcgacag aaggagcagt caaaaatggc
     2761 gcgcgtaaaa caattgaaca cgaagcaaaa acccaaaata ccgccggtcg atttggatcc
     2821 gttgtcgggc aattggccgc agctgctcgt tgcgttgcgc caactcgcac aggccggcga
     2881 gcttcattcg cggcatctga tcagcgcctg cggcctgaca gttccacaga ccttggtgtt
     2941 gcgcgccatc gacgggctcg agcaagcgac ggcggggcgg atctcgcaac tggtgagcct
     3001 cagtccgccg acggtgacgg caatccttaa tcgccttgag gagcggcaac tggtacagcg
     3061 cgaccgtggc gcttcggatc gccggttggt cattctcagc ctgacatcgc gcggacgaga
     3121 ggtcctggcc actgcgccga gcctcttgcc gcccgctttc atggcgcgct tcgcggcgct
     3181 accgctctgg gagcagacac tgctgctctc ctcgttgcag cgactcgcgg agatgatggg
     3241 agagggtgcg ccggcagcgc tcgacgccgt ggcggacgcg gggacatcgg tcgctggata
     3301 gccgagccgg aaagcgagcg ggattggcag cccgattccg gggccggaaa aggaggcccc
     3361 gaagcgcggc taacgggcgg gtgcttgcgg gggtattcgc gtcgccggcc gccgggccga
     3421 cgatgcctca ggcgacgagg gcgcggttgg cgacgagtat gccgtcggcg tcggcgtaga
     3481 gccactcgcc gggcgcaaag ctgacgccac cgaaggtgac gacgacatcg cgctggccga
     3541 cgccggcctt gacgctttta cgcggatggg tgtcgagcgc gcggacgccg atcgcctgtt
     3601 tggcgatgac cgccgagtcg cggatgcagc cgtagacaac gatgccggcc cagccattct
     3661 tcacgccgag ttcaccgagt tggtcgccga ccagcgcgca acggcggctg ccgccaccat
     3721 cgaccaccag gacgcggcct tcaccgggct cgccgagacg ttcgcgcacc agtgagttgt
     3781 cttcaaagac ctttagcgtg gcaatcgggc cggcgaatgc cggcacgccg ccgtaggtcc
     3841 tgaacatcgg ctctacgacg cgcaggccgt attcgggttt gccgtcgaac tcgtcgcaga
     3901 gatcgcaggt ggcgaaggcg ggtttggaac tcatcgcgtc gacctcagac gcaggcgccg
     3961 tcgtcggtaa caccttcggg tttttccttg ggcggcttga tgaactgctc gcgcgagacg
     4021 cccagccaca tcaccagcgg gctggcaacc agcaccgagg agtagatgcc gaaaaggatg
     4081 ccgatggtga gtgcgacggc gaagtagtgc agggcctcgc cgccgaagat cagcatcgac
     4141 agcaccatga tctgcgtcga accgtgggtg atcaccgtgc gtgaaatcgt ccgtgtgatc
     4201 gcatggtcaa gcacctgcgg cgtggtcagg ccgcgggtct tgcgaaaggt ctcgcggacg
     4261 cggtcgaaga cgacgaccga ctcgtttact gagtaaccga gcaccgccag cacggcggcc
     4321 agtaccggca gcgagaactc ccactgaaag aaggcaaaga agccgaggat gatgatcacg
     4381 tcgtgcaggt tggcgacgat cgccgagact gagaaacgcc actcgaagcg gaaggcgagg
     4441 tagacgacga tgccgatgat gaccagcagc agcgccagcg cgccgttctc gatcagttcg
     4501 cgaccgacct gcgggccgac gaactcgacg cgcgacagcg tgacgtccgg actggcctgc
     4561 gacagcgccg ccatcaccga atcgccgacg tgggccgaac tctgttcggt gcgcagcggc
     4621 aggcggatca gcacgtcgtt ggtggaaccg aaatgctgaa cggccatgtc ggtgtaccca
     4681 gcctgcgaca gggcgccgcg aacggaatcg aggtcggccg actcgctgta gcgcgcttcg
     4741 accagcgtgc cgccggtgaa ctcgaccgaa aggtgcagcc cgcgcatggt gaggaagaac
     4801 acggcaagtg cgaaggtgac cagcgagatg atgttgaaca ccagcgcatg gcgcatgaac
     4861 gggatgtcgt tccggatgcg gaagaattcc atgattgcct acctcactgt gccgcttcgg
     4921 gagcgggatt ggcggtcggt tt
//
LOCUS       DATDAA010000082         7456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_495104,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000082 DATDAA010000000
VERSION     DATDAA010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495104"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VIS73_02680"
     CDS             complement(<1..599)
                     /locus_tag="VIS73_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762090.1"
                     /translation="MRWHSPAVVDTVGVRGRRIAAALLAASLLSACSTFSDRIARVEQ
                     RLVAGDFDAAAAALERIDFAERDQALLLLNRGMLARYRGDLVASNADLEAAKQRMQAL
                     AALSIREGVLASTVTEQAGSYAGQPFERLLVPVFKAFNYLDLGDPDAARVEALQIDLL
                     LRELGEGRDEATVAGAAFARYVAGLVYELGGERSDAFIA"
     gene            complement(650..1255)
                     /locus_tag="VIS73_02685"
     CDS             complement(650..1255)
                     /locus_tag="VIS73_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HEY5762091.1"
                     /translation="MNAGTIRAAGLALLLAGLLGACSTSVERIDSAEVRDLSGAWNDT
                     DSRLVSEEMIADVLTRPWIDNHERNARKPPVVIVGEVRNLSHEHINTVTFVADLERAL
                     INSGRVEMVASSKERGEIRGERKDQDLNAREDTRKEMGQEIGADYMLKGTINTIIDVD
                     GKDQIRFYQVDLNMISLADNRKVWQGQKKIKKDVKRAKIRF"
     gene            complement(1252..1806)
                     /locus_tag="VIS73_02690"
     CDS             complement(1252..1806)
                     /locus_tag="VIS73_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762092.1"
                     /translation="MKIGTIAVVALLALAGCAGQTKLESDLGIKGAPDWVNKGTAYVS
                     NDSGRLFHGVGSAGQMGDASLQRSVADDRARAELAKIFSAYMDVVATDYQSAAKAGGA
                     SAADESVSRQIRALSQVNLAGAKVIARWIDPKQKVVWSIAEIDLKQVKDTAAVAAGMN
                     GDLQRYVGAKAENVFDQVVKGGKR"
     gene            1954..2382
                     /locus_tag="VIS73_02695"
     CDS             1954..2382
                     /locus_tag="VIS73_02695"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HEY5762093.1"
                     /translation="MSLIRWTDKFAVNQSLIDDQHRYLFQLINDFHDAYMQRQTRQDV
                     LILLNRLIDYAQRHFEDEERIMAEKAYPQLEAHQALHVKLFEEIFALNEKLADRALNP
                     TRDTIAFLKNWLTDHIMLHDLKFAEHLNASGAAGAGTAKV"
     gene            complement(2377..2835)
                     /gene="rnpB"
                     /locus_tag="VIS73_02700"
     ncRNA           complement(2377..2835)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VIS73_02700"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2872..3291)
                     /locus_tag="VIS73_02705"
     CDS             complement(2872..3291)
                     /locus_tag="VIS73_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762094.1"
                     /translation="MSITFGKRLLLGLAAPLLAGCISDAASYIITPPDHALTLIREQR
                     WFWNDSVELEIVAARMPDCQRRHKLDPAPAAEATVEVYQTGSTTFVLKQGARFYLAET
                     RSCVGFEKLDTPPPGGIGQPIGIFREEGGKLRFAKAV"
     gene            complement(3305..4357)
                     /gene="pyrC"
                     /locus_tag="VIS73_02710"
     CDS             complement(3305..4357)
                     /gene="pyrC"
                     /locus_tag="VIS73_02710"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY5762095.1"
                     /translation="MKETPSETLSLTRPDDWHLHLRDGAALVAVLPHTARQFARAIVM
                     PNLKPPVTTVAQAAAYRERILAALPAGAEFTPLMTLYLTDRTSVDEIRRAADSDFVHA
                     VKLYPAGATTNSDAGVTDLRHCHAALAEMEKAGLPLLVHGEVTDPAIDLFDREAVFIE
                     RVLAPLLSEHPGLRVVMEHITTADAADFVRAVSANVAATITAHHLIYNRNAIFQGGVR
                     PHWYCLPVLKRETHRLALVAAATSGSPKFFLGTDSAPHAKAAKEAACGCAGCYTAHAA
                     LELYAAAFEAAGALDRLEGFASHFGADWYGLPRNKSRVTLQKSPWQTPLAYPYVDGET
                     LVPLAAGETLSWRFVG"
     gene            complement(4374..5237)
                     /gene="rsmI"
                     /locus_tag="VIS73_02715"
     CDS             complement(4374..5237)
                     /gene="rsmI"
                     /locus_tag="VIS73_02715"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY5762096.1"
                     /translation="MTNELPAFYVVATPIGNLADITLRALTVLRGVGLIAAEDTRHTR
                     HLCTHHGISAALIAAHEHNEARAAGEILSRLAAGQSVAYVSDAGTPAISDPGARLVAA
                     VRAAGHPVVPLPGPCAAVTAMSVSGFADGAWTFRGFLPHKTAARRTVLESLVALPEIQ
                     IFYESTHRIVDSLAAIGEVFGERRIVLAKELTKVFEAIVDGTAAEVLDWLAADGERAR
                     GEFVLVIAGAAADAKPDDSARVLALLLADGLPVRQASNLAAAISGAPRKKLYEQALAL
                     RAQSATLDPDN"
     gene            5268..5489
                     /locus_tag="VIS73_02720"
     CDS             5268..5489
                     /locus_tag="VIS73_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY5762097.1"
                     /translation="MGGKDSTRAKPSGAANARELSLAAESITPAKIRRIVLATRLYLA
                     ALPARFDCVLVDAGAEADVRWLRDAFSAD"
     gene            5560..6153
                     /locus_tag="VIS73_02725"
     CDS             5560..6153
                     /locus_tag="VIS73_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY5762098.1"
                     /translation="MELVQRVGRLFDDSLAVQSATRDALAPDLARAVEVLTATLLEGG
                     KILVCGNGGSAAQAQHFACELIDCYETERQELAAINLCADTTLLTAIGNNHGQAQLFA
                     KQINALGQAGDLLVVLSTSGNSESVIEAIHAAHRHELRVIALTGASGGRLAAILGDSD
                     LLLAVPDDRTPRVQEAHQLMLHCLCDGVDALLLGLET"
     gene            6150..6821
                     /locus_tag="VIS73_02730"
     CDS             6150..6821
                     /locus_tag="VIS73_02730"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY5762099.1"
                     /translation="MKLTTKHLLLALAIACLPALQGCFPVAAVGMTASALLVSDRRTV
                     AAQAVDQELEYRIGRHLETDISKNAHVNVTSFNQTVLLTGEVPNEAERAKVEAIAKSM
                     PYSPKVVNELQVRPASSLVSRSDDALLTSTIKSRLAGSDVVLPNHVKIISEDGVVFVM
                     GLVTEREARSALQIAAESTGVVKVVNVTQLIRDPEEVRREEEAKRPLPPDPETTTPLW
                     RPQRF"
     gene            complement(6826..7269)
                     /locus_tag="VIS73_02735"
     CDS             complement(6826..7269)
                     /locus_tag="VIS73_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5762100.1"
                     /translation="MALPEQTLKTLIAAYESLSPQSIDALLACYAENARFRDPFNDVV
                     GRRAIGAVFAHMFETVEKPSFRVSGRFDSADAVVLRWEFGFHSARLGGAQAITGLSTL
                     RFDSDGLVVEHLDYWDASGNLYEKLPLIGGLLRWLRRSLAAPQGD"
BASE COUNT         1277 a         2530 c         2398 g         1251 t
ORIGIN      
        1 taggcgataa aggcgtccga gcgctcgccg ccgagttcgt agacgaggcc cgcgacgtag
       61 cgcgcaaagg cggcgccggc gacggtcgcc tcgtcgcggc cttcgccgag ttctcgcagc
      121 agcagatcga tctgcagcgc ctcgacacgg gccgcatcgg ggtcgccgag gtcgaggtag
      181 ttgaaggcct tgaacaccgg caccagcagg cgctcgaagg gctgtccggc gtaggaaccg
      241 gcctgttcgg tgactgtgct ggcgagcacg ccctcacgaa tgctcagcgc cgccagtgcc
      301 tgcatgcgct gcttggcggc ctccagatcg gcgttcgagg ccacgagatc gccgcggtag
      361 cgggcgagca tgccgcgatt gagcagcagc agggcttggt cgcgctcggc gaaatcgatg
      421 cgctccagcg cggcggctgc agcgtcgaag tcgccggcga cgaggcgttg ctcgacccgg
      481 gcgatccgat cactgaaggt cgagcaggcc gagagcaggc tcgcagcgag cagcgccgca
      541 gcgatgcgcc gcccgcgcac gccgaccgta tcgaccacgg cgggcgaatg ccaccgcatc
      601 cggatcgcct ggcgcagttc cggcacgagc cgacccgatt cacgcgcgct cagaagcgga
      661 tcttggcgcg cttgacgtcc ttcttgatct tcttctggcc ctgccagacc ttgcggttgt
      721 cggcgagact gatcatgttg agatcgacct gatagaagcg gatctgatcc ttgccgtcga
      781 cgtcgatgat ggtattgatc gtgcccttaa gcatgtaatc ggctccgatc tcctgtccca
      841 tctccttgcg cgtatcctcg cgggcgttga gatcctgatc cttgcgctcg ccgcggatct
      901 cgccacgctc cttgctcgac gccaccatct caacgcgccc ggagttgatc agcgcccgtt
      961 ccagatcggc gacaaaggtc acggtattga tgtgctcatg cgacagattg cgcacctcgc
     1021 cgacgatcac caccggcggc ttgcgggcgt tgcgctcgtg gttgtctatc cacggccgcg
     1081 tcagcacgtc ggcgatcatc tcctcggaga ccagccgcga atcggtgtcg ttccaggcac
     1141 ccgagaggtc gcgaacctcg gcgctatcga tgcgttcgac cgaggtgctg caggcgccca
     1201 gcaagcccgc cagcaacagc gccagccccg cggcgcgaat cgtaccggcg ttcatcgctt
     1261 gcctcccttc actacctgat cgaagacgtt ctcggccttg gcaccgacgt agcgctgcag
     1321 atcgccgttc atgccggccg caaccgccgc cgtgtccttg acctgcttga ggtcgatctc
     1381 ggcgatcgac cagacgacct tctgcttcgg atcgatccag cgcgcaatga ccttcgcgcc
     1441 ggccaaattg acctgcgaca gcgcgcggat ctgccgcgac accgactcgt cggcagcgct
     1501 ggcgccaccg gccttcgccg ccgactggta gtcggtggcg acgacatcca tgtaagccga
     1561 gaagatcttc gccagttcgg cgcgcgcccg gtcatcggcc accgagcgct gcagcgaagc
     1621 gtcgcccatc tggccggccg aaccgacccc gtgaaagagc cgcccgctgt cgttgctgac
     1681 ataggccgtg cccttgttaa cccagtcggg cgcccccttg ataccaaggt cgctctcgag
     1741 cttggtctgc ccggcacagc cggccagcgc cagcagcgcc actaccgcga tcgttcctat
     1801 tttcatgttg atctccagtc tcgcccgcaa tgcgaacatg ccagcgacga ttataagtgg
     1861 gcgctgtggc gtcgaccgcc gcttgtcgcc ggcgccggct tgcccgtacg atgcaccctg
     1921 attctctgac ctaaacgtgc cgccgaggtt gccgtgagcc tgatccgctg gaccgacaag
     1981 tttgccgtca accagagcct aatcgacgat cagcaccgct atctgttcca gctgatcaac
     2041 gacttccacg atgcctatat gcaacgacag acgcggcagg acgtgttgat cctgctcaac
     2101 cggctgattg actacgcgca gcggcatttt gaggacgagg aacggatcat ggcggagaag
     2161 gcctacccgc agctcgaggc gcatcaggcg ctgcacgtga agctgttcga ggagattttc
     2221 gcgctcaacg agaaactcgc cgaccgcgcg ctcaatccga cgcgcgacac gatcgccttc
     2281 ctcaagaact ggctaaccga ccacatcatg ctgcacgacc tgaaattcgc cgagcatctc
     2341 aatgcctcag gcgcagctgg cgccggtaca gcgaaagtgt gaagccggcc gataagccgg
     2401 gtcctgtgtc aggcgttggc cgaaaccttc gccccaggca gtcattcctc taggccctgc
     2461 gttgccgcag ggctcaagca gcctacccgg gagcagcgcg agccacgcta tcgctcccct
     2521 atttggcctt gctccggatg gggtttgccg tgccgtccgg cgttgtcccc ggacgcggtg
     2581 cgctcttgcc gcaccttttc acccttgcct ggcgaccgac gccatcggcg gtattttctc
     2641 tgttgcctga gccagccgcc cgggacgaat ccaccgggtt gccccggctc gatcgacgaa
     2701 ctgtctcagg tcctccctgc ggaggacact ttccatcacc tcacggtgtc cggccgttag
     2761 ccggcatcct gctctgcgga gcccggactt tcctcccccc cgatgactcg gggcggcgac
     2821 tgcctggccg gcttcacggc cgcgattata ggcgcggccc cgctgctgcg ctcaaactgc
     2881 cttggcgaaa cgcagcttgc cgccttcctc tcggaagatg ccgatcggtt ggccgatgcc
     2941 gccgggcggc ggcgtgtcga gcttttcgaa gccgacgcag gaacgcgtct cggcaagata
     3001 aaaacgcgcg ccctgcttga gcacgaaggt ggtgctgccg gtctggtaga cctcgaccgt
     3061 ggcctcggca gccggcgccg gatcgagctt gtggcggcgc tggcagtcag gcatgcgggc
     3121 ggcgacaatc tcgagttcga ccgagtcgtt ccagaaccag cgctgctcac ggatcagcgt
     3181 cagcgcatgg tccggcggcg tgatgatgta cgaggctgcg tcactaatgc agccggccag
     3241 cagcggcgct gcgaggccaa gcagcagtcg tttcccgaat gtgatcgaca tcgttccccc
     3301 ttgcttatcc gacgaagcgc cacgacaggg tttccccggc ggccagcggc accagcgtct
     3361 cgccgtcgac ataaggatag gccagtggcg tctgccacgg gcttttttgc agcgtcacgc
     3421 gcgatttgtt gcgcggcaac ccgtaccagt cggcgccaaa gtggctggcg aagccttcga
     3481 ggcgatcgag cgcgccggca gcctcgaagg ccgcggcata gagttcgagc gcggcgtgcg
     3541 cggtgtagca gccggcacag ccgcaggcag cctccttggc agccttcgcg tgcggcgccg
     3601 aatcggtgcc gagaaagaat ttcggactgc cggaggtggc tgcggcgacc agtgccagac
     3661 gatgcgtctc gcgcttcagt accggcaggc agtaccagtg cgggcgcacg ccgccctgga
     3721 atatcgcgtt gcggttgtag atcaggtggt gcgcggtgat cgtcgccgcg acgttggcgc
     3781 tgactgcgcg cacgaaatcc gcggcatcag cggtggtgat gtgctccatg accacgcgca
     3841 gccccggatg ctcgctgagc agcggcgcga gcacgcgctc gatgaacacg gcctcacggt
     3901 cgaacaggtc gatcgccggg tcggtgactt cgccgtgaac gagcagcggc aggcctgcct
     3961 tctccatctc ggcgagcgct gcgtggcagt ggcggagatc ggtaacgccg gcatccgaat
     4021 tggtagtcgc cccggccggg tagagcttga ccgcgtgcac aaagtcgctg tcggcagcgc
     4081 ggcggatttc gtcgacgctg gtccggtcgg tgaggtagag cgtcatcagc ggcgtgaact
     4141 cggcaccggc cggcagcgcc gcaaggatgc gctcgcggta ggcggcagcc tgcgccaccg
     4201 tggtcaccgg cggcttcagg ttgggcatga cgatggcgcg ggcgaactgg cgcgccgtat
     4261 gcggcaggac ggcgacgagc gccgcgccgt cgcgcaggtg taggtgccag tcgtcggggc
     4321 gggtgaggct cagggtttcg ctgggggtct ctttcacgcg cgggaatcct cggctagttg
     4381 tcggggtcga gcgtagccga ttgcgcgcgc agcgcaagcg cctgctcgta gagtttcttg
     4441 cgcggggcac cgctgatcgc cgctgcgaga ttcgaggcct gccgcaccgg caggccgtcg
     4501 gcaagcagca gcgcaagcac gcgcgcgctg tcgtcgggct tggcatcggc ggcggctccg
     4561 gcgatgacca gaacaaactc gccgcgcgcc cgctcgccgt cggcggccag ccagtcgaga
     4621 acctcggcag ccgtaccgtc gacgatcgcc tcgaagacct ttgtcagttc cttggccagc
     4681 acgatgcgtc gctcaccgaa gacctcgccg atcgccgcca ggctgtcgac gatgcggtgg
     4741 gtcgattcgt agaagatctg gatctcgggc agcgccacca gcgattcgag cacggttcgt
     4801 cgcgcggcgg tcttgtgggg cagaaagccg cggaatgtcc aggccccatc tgcgaagccg
     4861 gaaaccgaca tcgcggttac cgcggcacat ggcccaggca gtggcacgac gggatgaccg
     4921 gcagcgcgaa ctgccgcgac caggcgggca cctggatcgg aaatcgccgg cgtgccggcg
     4981 tcggaaacgt aggcgaccga ctggccggca gcgaggcgcg ataggatttc gcccgcggct
     5041 cgcgcttcat tatgctcgtg ggcggcgatc agggccgccg aaatcccgtg gtgcgtacac
     5101 aggtggcggg tgtggcgggt gtcctcggcg gcgatcaggc cgacgccacg caacaccgtc
     5161 agcgcccgca gcgtgatgtc ggcgagattt ccgatcggcg tcgcgacgac atagaatgcc
     5221 ggcaattcgt tcgtcatcga ttcgggcgca gcgggggaag gcacggcatg ggcggcaaag
     5281 atagcacgcg cgctaaaccg tcaggcgccg cgaacgcccg tgagctaagc cttgccgccg
     5341 agagcatcac gccggcgaag atccggcgca tcgtcctcgc cacccgactc tacctcgccg
     5401 ccctacccgc gcgtttcgac tgcgtgctgg tcgacgccgg cgctgaggcc gacgtacgct
     5461 ggctgcgcga cgcgttctcc gccgactgag cccgggcgca agcagcccgg ctgctagaat
     5521 ccgggccctt gtcattgcct ttgccggttg cccccagcca tggaactcgt ccagcgcgtc
     5581 ggccgtctgt tcgacgacag ccttgccgtt caatccgcga cccgcgatgc gctggcgcct
     5641 gatctggcgc gcgccgtcga ggtactcact gccaccctgc tcgaaggcgg caagatcctc
     5701 gtctgtggca atggcggctc ggcggcgcag gcccagcatt tcgcctgcga actgatcgac
     5761 tgctacgaaa ccgaacggca ggagctggcg gcgatcaacc tctgcgccga caccacgctg
     5821 ctcaccgcaa tcggcaacaa ccacggccag gcgcagctct ttgccaagca gatcaacgcg
     5881 ctcgggcagg ccggcgacct gctggtcgtc cttagcacga gcggcaactc ggaaagcgtg
     5941 atcgaggcaa tccacgccgc ccaccgccac gaactccgcg tcatcgccct caccggcgcc
     6001 agcggtggcc ggctcgctgc catactcggc gacagcgacc tgctgcttgc cgtccccgac
     6061 gatcgcacgc cgcgcgtcca ggaagcacac cagctgatgc tgcactgcct gtgcgacggc
     6121 gtcgacgccc tcctcctcgg actggaaaca tgaaactgac cacgaaacat ttgcttcttg
     6181 cactcgccat tgcctgcctg ccggccttgc agggctgctt tccggtcgcc gcggtgggca
     6241 tgactgcgag cgcactgctg gtctccgatc gacgcacggt ggcggcgcag gccgtcgacc
     6301 aggagcttga gtaccgcatc ggtcgccacc tcgaaaccga catcagcaag aatgcccacg
     6361 tcaacgtcac cagcttcaac caaaccgtgc tgctcactgg cgaagtaccc aacgaagccg
     6421 agcgcgccaa ggtcgaggcg atcgccaagt cgatgcccta ctcgccgaag gtggtcaacg
     6481 agctgcaagt gcgcccggcc tcgtcgctgg tctcgcgcag cgacgacgcg ctgctgacca
     6541 gcacgatcaa gtcgcggctg gcgggcagcg acgtcgtact gccgaaccac gtgaagatca
     6601 tcagcgagga cggcgtcgtc ttcgtcatgg gcctagtcac cgagcgtgag gcgcgcagcg
     6661 cgctgcagat cgccgccgaa tcgaccggcg tcgtcaaggt cgtcaacgtc acccagttga
     6721 ttcgcgaccc cgaggaagtc cgtcgtgaag aggaagccaa gcggcccctg ccgcccgacc
     6781 ccgagaccac aacaccgctc tggcggccgc agcgcttcta gacgatcagt cgccctgcgg
     6841 agcagccagc gaccgccgca gccagcgcag cagcccgcca atgagcggca gcttctcgta
     6901 gaggttgccg gatgcatccc agtagtcgag gtgctcgacg accagcccgt cggagtcgaa
     6961 gcgcagcgtc gacagtccgg tgatcgcctg cgcgccacca agccgggcgc tgtggaagcc
     7021 aaactcccag cgcagcacga cggcgtcggc cgaatcgaaa cggccgctca ctctgaatga
     7081 cggtttctcc accgtctcga acatgtgcgc gaagacagcg ccaatcgccc ggcggccgac
     7141 gacatcgttg aacggatcgc ggaaacgcgc gttctccgca tagcaggcga gcagtgcatc
     7201 aatgctctgc gggctcagcg attcgtaggc cgcaatgagc gtcttcagcg tctgttcggg
     7261 gagtgccatg ccgcttctcc gtttcggtaa aggctactct gccggcgcgg tgcgccgcat
     7321 cagcgcgaaa tagagccttg ccggcaacat gccgagcaat ttcatgaaac gcgaaaaccg
     7381 cttcggaaag tgaatttcga aaccgccgct ggcgagcccg tcgatgattt cccgcaccgc
     7441 ctcgtcgacg ccaatc
//
LOCUS       DATDAA010000083         9970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_180599,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000083 DATDAA010000000
VERSION     DATDAA010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9970
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180599"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /gene="mutM"
                     /locus_tag="VIS73_02740"
     CDS             <1..885
                     /gene="mutM"
                     /locus_tag="VIS73_02740"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY5762101.1"
                     /translation="LCQRRILPSTPSASAPPIMPELPEVEVSRLGIAPEIEGQTLAGA
                     IVRNRAFRQPVPAGLNRTLAGLRVESVRRRGKYLLLRCARGEQLAGHLLLHLGMSGSL
                     RFVARDAPAEAHDHVDLIFARQALRLRDPRRFGLVLWHAGADIDRHPLLATLGPEPLE
                     AEFDGAWLRRALASRTQAIKPAIMDSHLVVGVGNIYASESLFGARISPLRAANRISLA
                     GCERLAESIRRTLATAIAAGGSSVRDYVHSDGGAGCFQLQCAVYERAGEACQGCGAPI
                     RTIRQAGRATYYCPRCQR"
     gene            1007..2962
                     /locus_tag="VIS73_02745"
     CDS             1007..2962
                     /locus_tag="VIS73_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEY5762102.1"
                     /translation="MSLSKNFEAYSQWRGGVAAQVERFRRWLNDNDLNEAEIEQRLQQ
                     LLLKLREDRLTVAFVAEFSRGKSELINAIFFSGYGNRMLPSSAGRTTMCPTELLYEES
                     RDPTIDLLPIQTRSTNASISEYKRFPEEWKSMEIDITSGEAMQKALHHVSDVLRVAPE
                     EAKRLGFSVGEDETTSFRINEDGLVEIPKWRHAIINFPHPLLKQGLVVLDTPGLNAIG
                     TEPELTLSLLPNAHAVLFILAADTGVTQSDLVIWKDYIRGNDSKSTGRLIVLNKIDGM
                     WDELKTAAAVDAEIARQVDTCADILDLPEGQIFPVSAQKALVAKVTRDDALLQKSRIL
                     TLERALASELVPSKMEIVRETAAAEFWNSFRRTHRVLDARLNDVNEQLRELSGLRGKN
                     KGVVQYMLGKVKNEKSEFEIGLQRYYAVRSVFSTQTTALFSHLSLDSLRDLTKESRAG
                     MQKTSFSMGLSGAMSGFFAEVRDNVRLAEKDVAEIMAMMTAIYKKFAIEHGLKLGTPT
                     NFSLLRYEKEIDRLERWCNSHLNTPLNLLTQEKRMLVNRFFEEVAVQVRRTVEHANQD
                     AEIWIKAIMSPMETQVREHQVQLRRRLESIRRIHLATDTLDERIGELEHAARGLQSQI
                     ASLQQVGAAMEAQLSSAKNRVAAAAAA"
     gene            complement(3054..3302)
                     /locus_tag="VIS73_02750"
     CDS             complement(3054..3302)
                     /locus_tag="VIS73_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEY5762103.1"
                     /translation="MSLIITDECINCDVCEPECPNNAITQGEEIYEIDPEKCTECVGH
                     FDTPQCVEVCPVDCIPKDANHEETEDQLWTKYHKLTGK"
     gene            complement(3344..3841)
                     /gene="coaD"
                     /locus_tag="VIS73_02755"
     CDS             complement(3344..3841)
                     /gene="coaD"
                     /locus_tag="VIS73_02755"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY5762104.1"
                     /translation="MLNRHWAIYPGTFDPVTRGHEDLVRRASELFERVTVAVAESRSK
                     RPFFPLADRVAMAREAVADCDNVEVVGFDGLLMEFVHACGAKVIVRGLRAVSDFEYEF
                     QMAGMNRNLYPEVETVFLTPGEQYMFISATMVREIAILGGDVSKFVRPDVAVRMAQRV
                     KELAA"
     gene            complement(3835..4374)
                     /gene="rsmD"
                     /locus_tag="VIS73_02760"
     CDS             complement(3835..4374)
                     /gene="rsmD"
                     /locus_tag="VIS73_02760"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY5762105.1"
                     /translation="MRIIGGAWRKSVISFPVTEGLRPTPDRVRETVFNWLGQDLGGLN
                     CLDLFAGSGAFGIEAASRGASRVVLVEKSREAAAALRANTERLGGVGRVELRQADALQ
                     FAALCAERFDVIFVDPPYGQGWLARLAPLLSRLAAPRARLYIEAEAPVIALDEWRATR
                     SGTAGQVHYQLLEREEASC"
     gene            complement(4439..5758)
                     /locus_tag="VIS73_02765"
     CDS             complement(4439..5758)
                     /locus_tag="VIS73_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY5762106.1"
                     /translation="MNSTAPRFAGALVAAFAFAAAAPVGAAIPIDNWTAATGAKVYFV
                     ASPGLPILDLQIAFPAGSAFDPPGKEGLAGMVTGTLDLGAGNLDENAIAEKLADLGAR
                     LGGSASSDRASVSLRTLSDLPTRNAALALLKAVITQPAFPAAVVAREKARAVAGLQDA
                     LTRPDTLAARAFDQALYGNHPYGRQTSVESLNAIGRDDLIAFHRERFTAPAAVVTIVG
                     AVTAAEARVIADDLTAGLPRTQPAMSLPPVALPAAQTIPVPHPATQAHVLIGTPAMRR
                     GDPDYFPLYVGNYVLGGGGFVSRLTNEIRDKRGLAYSVYSYFNPQKELGPMQIGLQTK
                     AEQADEAIALVRQTLDDFLRNGPTETELAAAKANLVGGFPLRLDSNRKILDNVAVIAG
                     YGLPLDWLDTYRDRVAAVTIDDIRAAFARRIKPEHLITVRTTRDAGG"
     gene            complement(5770..7146)
                     /locus_tag="VIS73_02770"
     CDS             complement(5770..7146)
                     /locus_tag="VIS73_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY5762107.1"
                     /translation="MSGNRGSSALRGLLLILLVSAPLAATANPYETTLDNGLKVVVKE
                     DRRAPTAAQMVWYKAGAMDEVDGVSGIAHLLEHMMFKGTPSVGPGEFNKRVAAAGGRD
                     NAFTSWDYTAYFQQVPKESLEEMMKLESDRMRHLTLDPKEFAQEAKVVMEERRMRTDD
                     EPTALLYEQLAATAFVAHPYRRPIIGWMNDIENLTPADARAWYDAWYVPNNATLVVVG
                     DIDHQEVFKLARKYYGAAKARPLPPRKPRVEPEQMGVRRVTVKAPAELPELYMAYKAP
                     ALREAATDREPYALQMLAAVLSGHDAARFSRRLVKEQGLAVSAGAHYDLTSRGPSLFF
                     LSGSPRPGRSVAEVEDALRAEIADVVLNGVSEAELARAKAQLVAGEIYKRDSTFAQAM
                     EIGMYETLGYGHRAVDRIIEGLKAVTAEEIRDAAARVFRDDALTVGTLVPQPLPNAPR
                     RPAAAVRH"
     gene            7348..8355
                     /gene="ftsY"
                     /locus_tag="VIS73_02775"
     CDS             7348..8355
                     /gene="ftsY"
                     /locus_tag="VIS73_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY5762108.1"
                     /translation="MFGFQKKDKTPAEVPTKAPVAAPVEAATEAPARSWRERLFSGLS
                     RSRAQLGGGLRTIFSRGKVDDELLEELETLLLSADVGIEATQHLLRELTTKAKKEKLD
                     GPEAIQAALADSLTKLLAPLEAPLDTATHKPFVIMVAGVNGAGKTTSIGKLAKQFQKH
                     GKSVILAAGDTFRAAAREQLMTWGERNNVAVVAQESGDPAAVVFDAIAAAKARGIDIV
                     IADTAGRLPTQLHLMEEIAKVRRVVQKAEPSAPHEVLLVLDANIGQNALMQVKAFDRA
                     VSVTGLIVTKLDGTAKGGVIAAIARQCPKPIRFIGVGETIDDLRPFVAREFVEALFDI
                     R"
     gene            8363..9016
                     /locus_tag="VIS73_02780"
     CDS             8363..9016
                     /locus_tag="VIS73_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5762109.1"
                     /translation="MIAAQSLTKRYPGSRANSVDGISLAIAAGEFIAVSGRSGAGKTT
                     LLKLLGAIEKPTGGSLVVNGQNLAALTPAALPFVRRNIGFVFQDQKLLYDRSALDNAR
                     LPLDIVGMAPREAQRRAQAALDKVGLAGRERTLPLALSGGEQQRLAIARAVVNRPALL
                     IADEPIASLDDETAADVMRVFKAFHEVGTTVIVASHDDAWAKRLAARPLHLAAGRVA"
     gene            9111..>9970
                     /locus_tag="VIS73_02785"
     CDS             9111..>9970
                     /locus_tag="VIS73_02785"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEY5762110.1"
                     /translation="MGGWFTVVTAWLTSHQRAARDALARLKAGAGSSALTVLALAVVL
                     VLPALGHWLVDNVERAGSRLAGPGELSIWLADGSGREDIAAIEKRLAEADITRWRFVS
                     KDDALASLQAVDGLAEVAAGLKDNPLPDSFIVEPRQSDAAGLGAFAEIARGWPKVAEV
                     HADVDWAQRLSALTGVARRALWLLSAILAVALVAVSFNTIRLQVAARQQEIEIALLVG
                     ATPAWAARPFLWLGTLEGLAAGLLAVLALYGIYAIAAPGLADLAGFFGEAAVFEAPSL
                     RLATTLLIGA"
BASE COUNT         1652 a         3445 c         3260 g         1613 t
ORIGIN      
        1 ctgtgtcagc gccgcatcct gcccagcacg ccatccgcaa gcgccccgcc gatcatgcct
       61 gaactgcccg aagtcgaagt cagccgcctc ggcatcgctc ccgaaatcga gggacagacg
      121 cttgccggtg ccatcgtccg caatcgtgcc tttcgccaac ccgttcccgc agggctgaac
      181 cgcaccctcg ccggcctgcg cgtcgagtcg gttcgtcgcc gcggcaagta cctgctgctg
      241 cgctgtgcgc gcggcgagca actcgccggc cacctgctgc tccatctcgg gatgtccggc
      301 agccttcgct tcgtcgcccg cgacgccccg gccgaggcgc acgatcacgt cgacctgatc
      361 ttcgcgcggc aggcacttcg cctgcgcgac ccgcgccgtt tcggccttgt gctctggcac
      421 gccggtgccg acatcgaccg ccacccgctg ctcgccacgc tcggcccaga gccgctcgaa
      481 gcggaattcg atggcgcctg gctgcgccgc gcgctggcct cgcgcacgca ggcgatcaag
      541 ccggcgatca tggattcgca cttggtcgtc ggcgtcggca acatctacgc ctcggagagc
      601 ctgttcggcg cccgcatctc gccgctacgc gccgccaacc ggatctcgct cgccggctgc
      661 gagcggctgg ccgagtcgat ccggcggacg ctggctacgg cgatcgccgc tggcggcagc
      721 agcgtccgcg actacgtgca cagcgacggc ggcgccggct gcttccagct gcaatgcgcc
      781 gtgtatgagc gcgccggcga agcctgccaa ggctgcgggg caccgatccg cacgatccgc
      841 caagccggtc gggcgactta ctattgccca cgctgccagc gctgaggacg ctgcagttcg
      901 ccgcgccacc tggtctaggc ggcttgattt gccgcaagcc ccgcgctgca gcggtgccgg
      961 cgccctgtga tagagtcgcg cggtccgatt cgagcccgca aaacgcatgt ccctgtcgaa
     1021 gaatttcgag gcctacagcc agtggcgcgg cggcgtcgcc gcgcaggtcg aacgcttccg
     1081 gcgctggctc aacgacaacg acctcaacga ggccgagatc gagcagcgcc tgcagcaact
     1141 gctgctcaag ctgcgtgaag accggctcac cgtcgccttc gtcgccgagt tctcgcgcgg
     1201 caagtcggaa ctaatcaacg caatcttctt ctcgggctac ggcaaccgca tgctgccctc
     1261 gtccgccggc cgcacgacga tgtgcccgac cgagctgctc tacgaggaat cgcgcgaccc
     1321 gaccatcgac ctgctaccga tccagacccg cagcaccaac gccagcatca gcgagtacaa
     1381 gcgctttccc gaggaatgga agtcgatgga aatcgacatc acctcgggcg aggcgatgca
     1441 gaaggcgctg catcacgtca gcgacgtgct gcgcgtcgcg cccgaggaag ccaagcgcct
     1501 cggcttctcg gtcggcgagg acgaaacgac gagcttccgc atcaacgaag acggcctcgt
     1561 cgaaattccc aagtggcggc acgcgatcat caatttcccg cacccgctgc ttaagcaagg
     1621 cttggtggtc ctcgacacgc cgggcctgaa cgcgatcggc accgaacccg agctgacact
     1681 ctcgctgctg cctaacgcgc acgcggtgct gttcatcctc gccgctgaca ccggcgtcac
     1741 gcagtccgat ctggtgatct ggaaggacta catccgcggc aacgacagca agagcaccgg
     1801 ccgcctgatc gtgctgaaca agatcgacgg catgtgggat gagctgaaga ccgccgccgc
     1861 ggtcgatgcc gagatcgccc gccaagtcga tacctgcgcc gacatcctcg acctgcccga
     1921 gggccagatc tttccggtct cggcgcagaa ggcactggtt gccaaggtca cccgtgacga
     1981 cgcgctgctg cagaagagcc gcatcctcac gctcgaacgc gcgctcgctt ccgaactcgt
     2041 gccgtcgaag atggaaatcg tccgcgagac ggccgccgcc gagttctgga actccttccg
     2101 ccgcacgcat cgcgtactcg acgcgcggct taacgacgtc aacgagcagc tgcgcgagct
     2161 atcgggcctg cgcggcaaga acaagggcgt cgtccagtac atgctgggca aggtgaagaa
     2221 cgagaaaagc gaattcgaga tcggcctgca gcgctactac gcggtgcgca gcgtcttctc
     2281 gacacagacc acggcactct tctcgcacct ctcgctcgat tcgctgcgcg acctgaccaa
     2341 ggagagccgc gccgggatgc agaaaaccag cttctcgatg ggactgtcgg gtgccatgag
     2401 cgggttcttc gccgaggtcc gcgacaacgt gcggctcgcc gagaaggacg tcgccgaaat
     2461 catggcgatg atgactgcga tctacaagaa gttcgccatc gagcacggcc tcaagctcgg
     2521 cacgccgacg aacttctcgc tgctgcgcta cgaaaaggaa atcgaccgcc tcgagcgctg
     2581 gtgcaactca cacctcaaca ccccgctcaa cctgctgacg caggagaagc gcatgctcgt
     2641 caaccgcttc ttcgaggaag tcgccgtgca agtgcgacgc accgtcgagc acgccaacca
     2701 ggatgccgag atctggatca aggcgatcat gagcccgatg gaaacgcagg tgcgcgagca
     2761 ccaggtgcag ctgcggcgcc gcctcgaaag cattcgccgc atccatctgg ccaccgacac
     2821 gctcgacgag cgaatcggcg aactcgagca cgccgcccgt ggcctgcaga gccagatcgc
     2881 gtcgctgcag caggtcggcg cggcgatgga agcgcagttg tcgtctgcga agaatcgcgt
     2941 cgcggcggct gcggcggcct gaataaaacg ccaccaagct gcctccccgc agccaaacga
     3001 aaaagccgcg gtacgccgcg gcttttttca tccaccggcc cgcaggccgt caatcacttt
     3061 cctgtgagct tgtggtactt ggtccagagc tggtcttcgg tttcttcgtg gttggcatcc
     3121 ttggggatgc agtcgaccgg acagacctcg acgcactgcg gcgtgtcgaa gtggccgacg
     3181 cactcggtgc acttctcggg gtcgatctcg tagatctcct cgccctgggt gatcgcgttg
     3241 ttcgggcact cgggctcgca cacgtcgcag ttgatgcatt cgtcggtgat gatcagggac
     3301 atcttggttt ccttcgttac gttacgcgtt caatctgatt cgttcaggcg gcgagttcct
     3361 tgacccgctg cgccatccgc accgcgacat cgggccggac aaacttcgag acatcgccgc
     3421 cgaggatcgc gatctcgcgg accatcgtcg ccgagatgaa catgtactgc tcgcccggtg
     3481 tcaggaacac ggtttcgacc tcgggataca gattgcggtt catgcccgcc atctggaatt
     3541 cgtactcgaa gtcggacacc gcgcgcagtc cacggacgat gaccttggcg ccgcaggcgt
     3601 gcacgaactc catcagcagg ccgtcaaaac cgacgacctc gacgttgtcg cagtcggcga
     3661 cggcctcgcg ggccatggcc acgcggtctg cgagcggaaa gaatggacgc ttcgatcggc
     3721 tttcggcgac cgcgacggtg acccgctcga acagctcgct ggcgcggcga acaaggtcct
     3781 cgtgtccgcg cgtcaccggg tcgaaggtgc cgggatagat cgcccaatgc cggttcaaca
     3841 tgacgcttcc tcccgctcta gcaactggta gtgcacttgt cccgccgttc ccgagcgggt
     3901 ggcccgccat tcgtccagcg cgataaccgg cgcctcggct tcgatgtaca ggcgcgcccg
     3961 cggcgcagcc agtctgctca gcagcggcgc cagtcgcgcc agccatccct gtccgtaagg
     4021 cgggtcaacg aagatcacgt cgaagcgctc agcgcacagg gcggcgaatt gtagcgcatc
     4081 ggcctgtcgc agctccaccc ggcctacgcc accaagccgt tcggtattgg cgcgcagggc
     4141 cgcggccgct tcacggctct tctccaccag cacgactcgc gaggcgccgc gggaagcggc
     4201 ttcgataccg aaggcgccgc tgccggcaaa gaggtcgaga cagttgaggc cgccaagatc
     4261 ctgtccaagc cagttgaaga cggtctcgcg gacgcgatcg ggcgtcggcc gcaggccctc
     4321 ggtgacggga aagctgataa cgcttttccg ccacgcaccg ccgatgatac gcacggtacc
     4381 ggttgccgcc gaccgcgccg ggcgtgcggt gccgcgcccg gcagccggtc gcgcgccttt
     4441 agccgccggc atcccgcgtg gtgcgcacgg tgatcaggtg ttcgggcttg atgcggcgcg
     4501 cgaaggcggc gcggatgtcg tcgatggtga cggcagcaac gcgatcgcga taggtgtcga
     4561 gccagtctag cggcagaccg taaccggcga tcacggcgac gttgtcgaga atcttgcggt
     4621 tgctgtcgag gcgcagcggg aagccgccga ccagattggc cttggctgcc gccagttcgg
     4681 tttcggtcgg gccgttgcgc aggaagtcgt cgagcgtctg gcgcaccagc gcgatcgctt
     4741 cgtcggcttg ctcggccttg gtctgcaggc cgatctgcat ggggcccagc tccttctgcg
     4801 ggttgaagta gctgtaaacc gagtaggcaa ggccgcgctt gtcgcggatc tcgttcgtca
     4861 gccgcgagac gaagccgccg ccgccgagca cgtagttgcc aacgtagagc ggaaagtagt
     4921 cggggtcgcc acgacgcatc gccggggtgc cgatcagcac atgcgcctgc gtcgccgggt
     4981 gcggcaccgg aatggtctgc gctgcgggca gcgccaccgg cggcagcgac attgccggct
     5041 gcgtgcgcgg caggccggcg gtgaggtcat cggcgatgac gcgcgcttcg gcggcggtga
     5101 ccgctccgac gatagtgacc acggcggctg gcgcggtgaa gcgctcgcgg tggaaggcga
     5161 tgagatcgtc gcggccgatc gcgttgagcg attccaccga cgtttgccgg ccgtagggat
     5221 gattcccgta gagggcctga tcgaaggcgc gggcggcgag cgtgtcggga cgcgtcagcg
     5281 cgtcctgcaa gccggcgacc gcgcgcgcct tctcgcgcgc gaccactgcc gccggaaacg
     5341 ccggctgcgt aatcacggcc ttgagcaggg ccagcgccgc gttgcgcgtc ggtaggtcgg
     5401 agagcgtgcg caacgagacg cttgcacggt cgctcgacgc cgatccgcca agccgggcac
     5461 cgaggtcggc gagcttttcg gcgatggcgt tttcgtcgag atttccggca ccgagatcga
     5521 gcgtgccggt gaccatgccg gcaaggccct ccttgcccgg cgggtcgaag gccgaaccgg
     5581 cagggaaggc gatctgcagg tcgagtatgg gcagtccggg cgaggcgaca aagtagacct
     5641 tggcgccggt tgccgcggtc cagttgtcga tcggaatcgc tgcaccaacc ggcgccgcgg
     5701 cggcgaaggc aaaggcggcg acaagcgcac cggcgaaacg gggagctgtg ctgttcatgg
     5761 tcgcgctcct cagtggcgga cggccgcagc gggccggcgc ggtgcgtttg gcaggggctg
     5821 aggcacgagc gtgccgaccg tcagtgcgtc gtcgcggaag acgcgggcgg cggcatcgcg
     5881 gatttcctca gcggtcaccg ccttcaggcc ctcgataatg cggtcgacgg cgcgatgccc
     5941 gtagcccagc gtttcgtaca tgccgatctc catcgcctgt gcaaatgtcg agtcgcgctt
     6001 gtagatctcg ccggcaacca gctgcgcctt ggcacgcgcc agttcagcct cgctgacgcc
     6061 attgagcacg acgtcggcaa tctcggcgcg caaggcgtcc tcgacctcgg cgacgctgcg
     6121 gccgggtcgc ggcgaacccg agaggaaaaa caggctgggt ccgcggctag tcaggtcgta
     6181 gtgggcacca gccgagacgg caagcccctg ctccttgacc agtcgccgcg agaagcgcgc
     6241 cgcatcgtgg ccggagagca cggcggcaag catctgcagc gcgtagggtt cgcggtcggt
     6301 ggctgcttcg cgcagcgccg gtgccttgta ggccatgtac aactccggca actcggccgg
     6361 cgccttgacc gtgacgcggc gaacacccat ctgctcgggt tcgacgcgcg gcttacgcgg
     6421 cggcagcggc cgtgccttgg ccgcgccgta gtacttgcgc gccagcttga agacctcctg
     6481 gtgatcgatg tcgccgacga cgaccagcgt cgcgttgttg ggcacgtacc aggcgtcgta
     6541 ccaggcgcgc gcatcggccg gcgtcaggtt ctcgatgtcg ttcatccagc cgatgatcgg
     6601 ccgccgatag ggatgcgcga cgaaggccgt cgccgccagc tgctcgtaaa gcagcgcggt
     6661 cggctcgtcg tcggtgcgca ttcgccgctc ttccatcacc accttcgcct cctgcgcgaa
     6721 ttcctttgga tcgagcgtca ggtggcgcat gcggtcggac tcgagcttca tcatctcttc
     6781 gaggctctcc ttcggcacct gctggaagta ggccgtgtag tcccacgagg tgaaggcgtt
     6841 gtcgcgtccg ccggccgccg ccacgcgctt gttgaattcg ccgggcccga ccgagggcgt
     6901 acccttgaac atcatgtgct caagcaggtg ggcaattccc gaaacgccgt cgacctcgtc
     6961 cattgccccg gccttatacc aaaccatctg cgccgccgtc ggcgcccgcc ggtcttcctt
     7021 gaccacgacc ttgaggccgt tgtccagcgt cgtttcgtaa ggattggcag tggcggctaa
     7081 tggcgccgac accagcagaa tcaacaacag tccccgcagg gcgctcgatc ctcgattgcc
     7141 tgacatcgtc ctctatccgt gaatgcaagt gagtactgat tgacgcgtta cgtcggttgc
     7201 cggtcttcaa gaccggcctt gcgcatctgc taggattcgc gccgacgcgc ttcgctgcaa
     7261 cgcgccgggc atcttagcag cacgcccgcc ggcccccggg gcctggcatc gccgccccgc
     7321 cgacacccga tacagacccg aagacccatg tttggtttcc agaagaagga caagacgcct
     7381 gccgaggtgc ccaccaaggc accggtcgct gccccggtcg aggcagctac cgaggcacct
     7441 gcgcgctcct ggcgcgagcg cctgttttcc ggcctgtcgc gttcgcgcgc ccagctcggc
     7501 ggcggcctgc ggacgatctt ctcgcgcggc aaggttgacg acgagttgct cgaggaactc
     7561 gagaccttgc tgctgagcgc cgacgtcggc atcgaggcaa cccagcatct gctgcgcgag
     7621 ctgacgacga aagcgaagaa ggagaagctc gacggccccg aagcaatcca ggcggcgctc
     7681 gccgacagcc tgaccaagct actcgcgccg ctcgaggcgc cgcttgatac agccacccac
     7741 aagcccttcg tgatcatggt tgccggcgtc aatggcgccg gcaagacgac ttcgatcggc
     7801 aagctcgcca agcagttcca gaagcatggc aagagcgtca tcctcgccgc cggcgacacc
     7861 ttccgcgccg ccgcgcgcga gcagctgatg acctggggcg agcgcaacaa cgtggcggtc
     7921 gtcgcgcagg aatcgggcga cccggctgcc gtggtcttcg acgcgattgc cgcggccaag
     7981 gcgcgcggca tcgatatcgt catcgccgat accgccggcc gactgccgac ccagctgcac
     8041 ctgatggaag agatcgccaa ggtccgccgt gtcgtgcaga aggccgagcc ttcggcgccg
     8101 cacgaggtgc tgctggtgct cgacgccaac atcggccaga acgcactgat gcaggtaaag
     8161 gccttcgaca gggccgtcag cgttaccggc cttatcgtca ccaagctcga tggcactgcc
     8221 aagggcggcg tcatcgccgc gatcgcccgg cagtgtccga agccgatccg tttcatcggc
     8281 gtcggcgaga ccatcgacga cctgcgcccc ttcgtcgccc gcgagttcgt cgaggcgctg
     8341 ttcgacatcc ggtgatggcg ccgtgatcgc tgcgcaatcg ctgaccaagc gctaccccgg
     8401 cagccgcgcc aactcggtcg acggcatcag cctggcgatc gccgccggcg aattcatcgc
     8461 cgtcagcggc cgctcgggcg ccggcaagac gacgctgctc aagctgctcg gtgcgatcga
     8521 gaaaccgacc ggcggctcgc tggtggtcaa cggccagaac cttgcggcgc tgaccccggc
     8581 ggcactgccc ttcgtccgcc gcaacatcgg cttcgtcttc caggaccaga agctgctcta
     8641 cgaccgcagc gcgctcgaca atgcgcggct gccgctcgac atcgtcggca tggcgccgcg
     8701 cgaagcgcag cgccgcgccc aggcagcgct cgacaaggtc ggcctcgccg gccgcgaacg
     8761 cacgctgccg ctggcgcttt cgggcggcga gcagcagcgg ctggcgatcg ctcgcgccgt
     8821 agtcaaccgc ccagcgctgc tgatcgccga cgaaccgatc gccagcctcg acgacgagac
     8881 cgccgcagac gtcatgcgtg tcttcaaggc cttccacgag gtcggcacca ccgtcatcgt
     8941 tgcctcgcac gacgacgcat gggccaagcg gctcgccgcc cgcccactgc acctcgctgc
     9001 cgggagggtc gcgtaatgct ggcactccgc ccgatcggcg ccgccgtacg gccaccgcaa
     9061 atcctggcgg cactgcacgt cagcagaccc acccttcccc gtgaaggggg atggggggat
     9121 ggttcaccgt cgtgacggcc tggctcacct cccaccagcg cgccgcacgc gacgcgctcg
     9181 cccgcctgaa ggccggcgcc ggcagcagcg cgctgaccgt gctggcgctt gccgtcgtgc
     9241 tcgtgctgcc ggcgctcggc cattggctgg tcgacaacgt cgagcgtgcc ggcagccggc
     9301 tggccggccc cggcgagctc agcatctggc ttgccgacgg cagcgggcgc gaggacatcg
     9361 cggcgatcga aaaacggctt gccgaggccg acatcacgcg ctggcgcttt gtctcgaagg
     9421 acgacgcgct ggcctcgcta caggccgtcg acggactcgc cgaggtcgcg gccgggctca
     9481 aagacaaccc gctgcccgac agcttcatcg tcgagccgag gcagtccgac gcggccgggc
     9541 tcggcgcctt cgccgagatc gcgcgcggtt ggccgaaagt tgccgaggtg cacgccgacg
     9601 tcgattgggc gcagcgactc tcggccctga ccggcgttgc ccgccgggcg ctctggctgc
     9661 tctcggcgat cctcgcggtg gcgctggtgg cggtaagttt caacaccatc cgcctgcagg
     9721 tcgcggcacg ccagcaggaa atcgagatcg cgctactggt cggcgcgacg ccggcctggg
     9781 cggcccgccc ctttctctgg ctgggcacgc tcgaagggct ggccgccggc ctgctcgcgg
     9841 tgctggcgct ctacggcatc tacgccatcg ccgcgccggg ccttgccgat ctggccggct
     9901 tctttggaga ggcggcggtg ttcgaggcgc cctccttgcg gctggcgact accttgttga
     9961 tcggtgccat
//
LOCUS       DATDAA010000084         3530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_215919,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000084 DATDAA010000000
VERSION     DATDAA010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215919"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /gene="murA"
                     /locus_tag="VIS73_02790"
     CDS             <1..1084
                     /gene="murA"
                     /locus_tag="VIS73_02790"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY5762111.1"
                     /translation="GQMGVQVTLDGPDGLEMSAREITSVEAPYDLVKTMRASILVLGP
                     LLARHGEAKVSLPGGCAIGARPVDQHIKGLQAMGAEIAVEHGYIHAKAARLRGARICT
                     DMVTVTGTENLMMAASLAEGETVIENAAREPEVVDLANCLVAMGAQISGAGTDIIRIR
                     GVSQLKGVTHRIMPDRIETGTYLCAAAITGGDIRLLGASASHLDAVVDKLLDAGCEIE
                     IERDALRLRGPQRLQPVSLRTAPYPAFPTDMQAQFMAINCVADGASTIRETIFENRFM
                     HAVELIRLGASIDINGNTAVVRGVGQLQGARVMATDLRASAGLVVAALAAEGETLIER
                     IYHLDRGYERIEEKLSRLGARIKRVR"
     gene            1205..1849
                     /gene="hisG"
                     /locus_tag="VIS73_02795"
     CDS             1205..1849
                     /gene="hisG"
                     /locus_tag="VIS73_02795"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY5762112.1"
                     /translation="MSGITIALSKGRIFDETLPLLAAAGIVPSENPETSRKLIIGTNC
                     ADVRVVIVRATDVPTYVEHGAADLGVAGKDVLIEHGGAGLYQPLDLKIAKCRMMVAVP
                     EGFDYAQAVRQGARIKVATKYVRTAREHFAAKGVHVDLIKLYGSMELAPLAGLADAIV
                     DLVSTGGTLKANNLVAVEAIMPISSRLVINQAALKLKQQRLAGILDAFTRAVGN"
     gene            1863..3188
                     /gene="hisD"
                     /locus_tag="VIS73_02800"
     CDS             1863..3188
                     /gene="hisD"
                     /locus_tag="VIS73_02800"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY5762113.1"
                     /translation="MSTAALKIRRFDSRDADFKARLDALLAFETAQDEGIERTVAAIL
                     ADVRSRGDAAVIEYTNRFDRLAVKSMSELEIPRAELDQALTALPTTQREALQAAAARV
                     RAYHQRQPLQGWQYEEESEALRGTLLGQKVTPLDRVGLYVPGGKAAYPSSVLMNAIPA
                     KVAGVGELIMVVPTPGGERNALVLAAAALAGVDRVFAIGGAQAVGALAYGTESVPQVD
                     KVVGPGNAYVACAKRRVFGVVGIDMVAGPSEILIVCDGRTNPDWVAMDLFSQAEHDEL
                     AQSILLSPDAVFVDRVRAAMDRLLAEMPRKEVIRASLEGRGALITVRDLDEACAIANR
                     IAPEHLELSIENAEVWVDQIRHDGAIFIGRYTSESLGDYCAGPNHVLPTSGSARFSSP
                     LGVYDFQKRSSLIRVSAVGAQTLGRIASTLAWGEGLPAHARSAEFRLDP"
     gene            complement(3185..>3530)
                     /locus_tag="VIS73_02805"
     CDS             complement(3185..>3530)
                     /locus_tag="VIS73_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5762114.1"
                     /translation="FNSYPLGRLAQIGAIAAIKDEDWFNETRGKVIASRHRLTAELTT
                     LGFVVLPSQANFVFASHRSRRADELAAALRERKVIVRHFKAARIDNHLRITIGDDVQC
                     DALLAALREILA"
BASE COUNT          588 a         1171 c         1196 g          575 t
ORIGIN      
        1 tgggcagatg ggcgtgcagg tgacgctcga tggcccggat ggactggaaa tgagtgcgcg
       61 tgagatcacc tcggtcgagg cgccctacga cctggtcaag accatgcgcg cgtcgattct
      121 cgtgctcggg cccttgctgg cgcggcacgg cgaagccaag gtttcgctgc caggcggctg
      181 cgccatcggc gcgcgaccgg tcgaccaaca catcaagggc ctgcaagcga tgggcgccga
      241 gatcgccgtc gaacacgggt acatccacgc caaggcggcg cggctgcgcg gtgcgcgaat
      301 ctgtaccgac atggtcaccg tcacagggac cgagaacctc atgatggcgg ccagcctcgc
      361 cgagggtgaa acggtgatcg agaatgcagc gcgcgaaccc gaggtcgtcg atctcgccaa
      421 ctgcctggtc gcgatgggcg cgcagatttc cggcgccggt accgacatca tccgcatccg
      481 cggcgtctcg cagctcaagg gcgtgaccca ccggatcatg cccgaccgca tcgaaaccgg
      541 cacctacctg tgcgcggcgg cgatcaccgg aggcgacatc cgcctgctcg gcgccagcgc
      601 ctcgcacctc gacgccgtgg tcgacaaact gctcgacgcc ggttgcgaga tcgagatcga
      661 gcgcgacgcg ctgaggttgc gcggcccgca gcggctgcag ccggttagcc tgcgcacggc
      721 accgtacccg gcttttccga ccgacatgca ggcgcagttc atggcgatca actgcgttgc
      781 cgacggcgcc tcgacgatcc gcgagacgat cttcgagaac cgcttcatgc atgccgtcga
      841 actgatccgt ctcggcgcct cgatcgacat caacggcaac accgcagtgg tccggggcgt
      901 cggccagctg cagggggcgc gggtaatggc caccgacctg cgcgcctcgg ccggcctagt
      961 cgtcgcggcg ctggccgccg agggcgagac gctgatcgag cgcatctacc acctcgaccg
     1021 cggctacgag cggatcgagg aaaagctgtc gcgactgggc gccaggatca aacgtgtgcg
     1081 ttaacggagc agtctgccgg cggagcaaga tgctgccgcg accgggttcg catcgcccgg
     1141 ccgcaaggca gccgggccgg gccagcaggt agaatgcgcg ctctttgcat tcacgggagt
     1201 tgccgtgtcg ggcatcacca tcgccctttc caagggccgg atctttgacg agacgctgcc
     1261 gttgctggca gcggccggca ttgttcccag cgaaaacccg gaaacctcgc gcaagctgat
     1321 catcggcacc aattgtgccg atgtgcgcgt ggtcatcgtt cgtgccaccg acgtgccgac
     1381 ctatgtcgag cacggtgccg ccgatctcgg cgtcgccggc aaggacgtgc tgatcgagca
     1441 cggcggcgcc ggcctctacc agccgctcga tctcaagatc gccaagtgcc ggatgatggt
     1501 cgcggtgccc gagggcttcg attacgcgca ggccgttcgt cagggcgcgc ggatcaaggt
     1561 cgcgacgaag tacgtgcgca cggcgcgcga gcatttcgcg gccaaggggg tgcacgtcga
     1621 tctgatcaag ctctatggct cgatggaact ggcgccgctt gccggtctgg ccgatgcgat
     1681 cgtcgatctg gtgagcaccg gcggcacgct caaggccaac aatctggtcg cggtagaagc
     1741 gatcatgccg atttcctcgc ggctggtaat caaccaggcg gcgctcaagc tcaagcagca
     1801 gcgcctggcc ggcatcctcg atgccttcac ccgggccgtc ggcaactgaa caggagctgg
     1861 agatgagcac tgccgcgctg aagatccgcc gattcgactc ccgggacgcc gatttcaagg
     1921 cacgcctcga cgcgctgctg gccttcgaga ctgcgcagga tgagggcatc gagcgcactg
     1981 tcgccgccat cctcgccgat gtgcgcagcc gcggcgacgc ggcggtcatc gagtacacca
     2041 atcgtttcga ccggctcgcc gtgaagtcga tgagcgagct ggagattccc cgcgccgagc
     2101 tggatcaggc gctgaccgcc ctgccgacga cgcagcgcga ggcgctgcaa gcggcggccg
     2161 cgcgagtccg tgcctatcac cagcgccagc cgctgcaggg ctggcagtat gaggaagaga
     2221 gcgaagcgct gcgtggcacg ctgctcggcc agaaggtcac gccgctcgat cgtgtcggcc
     2281 tctacgtgcc gggcggcaag gccgcctatc cctcgtcggt gctgatgaac gcgattccgg
     2341 ccaaggttgc cggcgttggc gagctgatca tggtcgtgcc gacgcccggc ggcgagcgca
     2401 acgcgctggt gctggccgcc gccgcgctgg ccggtgtcga tcgcgtcttc gccatcggcg
     2461 gtgcccaggc cgtcggcgcg ctcgcctacg gcaccgagag cgtgccgcag gtcgacaagg
     2521 tcgtcggtcc cggcaacgcc tacgtcgcct gcgccaagcg ccgcgtcttc ggggtcgtcg
     2581 gcatcgacat ggtcgccgga ccgtcggaaa tcctcattgt ctgcgacggc cggaccaatc
     2641 cggactgggt ggcgatggat ctcttctcgc aggctgagca cgacgagctg gcgcagtcga
     2701 ttctgctcag cccggatgcc gtcttcgtcg atcgcgtgcg ggcggcgatg gatcgcctgc
     2761 tcgccgaaat gccgcgcaag gaggtgattc gtgcctcgct cgaaggtcgc ggtgcgctga
     2821 tcacggtgcg tgatctcgac gaggcctgcg ccatcgccaa ccggatcgcc cccgagcacc
     2881 tcgaactgtc gatcgagaat gccgaggtct gggtagacca gatccgccac gacggtgcga
     2941 ttttcatcgg ccgctacacc tcggagtccc tgggcgatta ttgcgccggt cccaaccacg
     3001 tgctgccgac ctcgggcagc gcgcggttct catcgccgct tggcgtgtac gacttccaga
     3061 aacgctcgag cctgatccgc gtttcggcgg tcggcgcgca gacgctgggc cggatcgcct
     3121 cgacgctggc atggggcgaa ggcctgccgg cgcacgcccg ctcggctgag tttcgcctag
     3181 acccctaagc gagaatctcg cgcaacgccg cgagcagcgc gtcacactgc acgtcgtcgc
     3241 cgatggtgat gcgcaggtgg ttgtcgattc gcgccgcctt gaaatggcgg acgatcacct
     3301 tgcgttcgcg cagcgcggcg gccagttcgt cggcccggcg tgagcggtgg ctggcgaaga
     3361 cgaaattggc ctgcgagggc agcaccacaa aaccgagcgt cgtcagctcg gccgtcaggc
     3421 gatgtcgact ggcaataacc ttgccgcgcg tttcattgaa ccagtcctcg tccttgatcg
     3481 cggcaatcgc gccgatttgc gcgagacggc cgagcgggta tgaattaaag
//
LOCUS       DATDAA010000085         5233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_250815,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000085 DATDAA010000000
VERSION     DATDAA010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250815"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VIS73_02810"
     CDS             <1..573
                     /locus_tag="VIS73_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY5762115.1"
                     /translation="PMVAAGGGGEEVTGSLKETTALDAASVMRFAEKVIIVPGYGMAV
                     AHAQHKIWEMSAMLEEAGVEVKFAIHPVAGRMPGHMNVLLAEAGVPYDKIFDLDEING
                     EFAQTDVALIIGANDVVNPSARTDKSSPIYGMPILAADQARNVIVIKRGQGTGYSGVE
                     NALFYRDNCRMLYGSAQQVAADLIGHIKTM"
     gene            complement(692..1123)
                     /locus_tag="VIS73_02815"
     CDS             complement(692..1123)
                     /locus_tag="VIS73_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762116.1"
                     /translation="MFSPITIPYGAKMLFNEVRLKPGVSVDDVELTLAEMCNVVKDTY
                     GNEKGGFIAGQVFRFSGFISDEGSVESGRGEGDHLAIVTYWQSFDQHEASHADRIFRE
                     KFAELAAQCSETRELGYEMLWQGVPEHAVAEPQAASDRKAA"
     gene            1260..2429
                     /locus_tag="VIS73_02820"
     CDS             1260..2429
                     /locus_tag="VIS73_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762117.1"
                     /translation="MTTCGPREISTPFRPIPVAVPEGMKPNEFFNSSENLADLLHNNG
                     LLQTPENLLLYRKALGHSVEFDCSILYNSSRTILDPLGRPVRRTQLPEPVRRVWNRMN
                     TIVIDEMLASYPDPERALVLAGEASLDATWPLTSPGVPSIRMLHNHFIVFPMAALRAA
                     KHADVDDPNLTDGGQHSLFQAHMREVYRRFFDEALTLTTLRLLPTSESTLALTGYPQG
                     LPGWEIVGGAATIARPDFWSDYDAILKGFLDFYRSFFGQVSTRNAEPPAGLYFPGAVE
                     AILLANNDFLATAKKVRERCLADARYANALRWQPAFKQLIYRDERGRLVVTISQNSIG
                     NAITELLGVVVRRVPDAEAYAQHEPALLERLLALRTKLIDADIGHGIATGQWGAV"
     gene            2460..4154
                     /locus_tag="VIS73_02825"
     CDS             2460..4154
                     /locus_tag="VIS73_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762118.1"
                     /translation="MTESIPAAAPAADAWNPGIGPGIPPQFRRLETIFRPECSSSSPG
                     EIAELESLTGLPSEELTAFRPERLALHEVIVRVTADLAVAEGEIEENFGQNFRAIARL
                     LLAEAVEPNIARLAADWKAWRDGAAAEIARILETALSATPAEPKRRRWLDRLFAWKHS
                     AAGPSWPPDEADVVAGFRSTGLAAEHPETRAIYRSLYRVLGAIQIRRGRIGGDTALLA
                     RLVTDHVANDYGGQLLGTWLSPAFAATIERHGLTRVPPRDAPVLISLKGASAAGKSSL
                     RPMIKRLLYEEGIEPDGYATISPDVWRRLLLDYEALGDAYKYAGHLTSRELMVIDGKL
                     DRYIRRKATIDHAIPHLLVDRFRFDSFESEKIGRVLHETYARHVDTLYMYFVITPPEE
                     TVLRGWQRALERGRYKAVEDFLGHSVEAYSGMPKVFFKWVAHERPLYRYSFLDNRVPK
                     GSFPRTIAFGTQREMTICDPLGLVDIARYQRIDVNAETADAVYPATEDFSVEANLGFL
                     DECLQQLPLIRFAETPDAPPYALRQSGQFAILEPERFAALRRVPDLDRVFAHILGE"
     gene            4236..>5233
                     /locus_tag="VIS73_02830"
     CDS             4236..>5233
                     /locus_tag="VIS73_02830"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEY5762119.1"
                     /translation="MKLSRPLLLAVSIWTLLVGASFAWNTLREYDEVFESARSDARTS
                     LDQDLSFRRWGTRHGGVYVPIDKHQQPNPWLSHIPERDLTTTDGRRLTLVNPAMMMRE
                     VMEDYGVEFGIEGRITGLKYLNPANAPDEWERAQLEAFARGERKETTEIVPLKGARHL
                     RHLRAMWMEPGCEKCHAVLGYKTGDLRGATGVNLPLAPYEARILGDLINLGVTHGLIW
                     LVGLAGIGWVTRDFSHRQAALRASEKRFRDLFDNSPDPCWLIQEDDVVDCNRAAAEML
                     GYRSIDEVLQNVDRLSPERQPDGQLSAVKSAAMAALARQNGVHRFEWEQRRADGSC"
BASE COUNT          957 a         1780 c         1577 g          919 t
ORIGIN      
        1 ccgatggtcg ctgctggcgg tggcggcgag gaagtgaccg gcagcctcaa ggaaacgacg
       61 gcgctcgacg ccgcatcggt gatgcgtttc gccgagaagg taatcatcgt ccccggttac
      121 ggcatggccg tcgcccacgc ccagcacaag atctgggaaa tgagcgcgat gctcgaggaa
      181 gccggggttg aggtgaagtt cgcgattcac ccggtcgccg gtcgcatgcc cggccacatg
      241 aacgtgctgc tcgccgaagc cggcgtgccc tacgacaaga tcttcgacct cgacgagatc
      301 aacggcgagt tcgcgcagac cgacgtggca ctgatcattg gcgccaacga cgtcgtcaat
      361 ccctcggcgc gtaccgacaa gtccagtccg atctacggaa tgccgattct cgccgccgat
      421 caggcgcgca acgtcatcgt catcaagcgc ggccagggaa ccggttattc aggggtcgag
      481 aatgcgctct tctaccgcga caactgccgc atgctctacg gcagcgcgca gcaggttgcc
      541 gccgatctca tcggccacat caagacgatg taagccggcc cgggccacga aaccgagccg
      601 ccttcgggcg cgaccggcgg tccgttccgg ccgcgattct gcggggctat tgctaggcga
      661 gcgtgcggac cttccgcgac gcggtctcgc cctacgcggc cttgcgatcc gaagcagcct
      721 gcggctcggc gaccgcgtgc tcgggcacgc cctgccagag catttcatac ccgagttcgc
      781 gggtctcgct gcactgcgcc gccagctcgg cgaatttctc gcggaagatg cggtcggcgt
      841 gcgaagcctc gtgctggtcg aaggattgcc aataggtgac gatcgccaga tggtcgccct
      901 cgccgcggcc cgattcgacc gagccctcgt cggagatgaa acccgagaac ctgaacacct
      961 ggccggcgat gaagccgcct ttctcgttgc cgtaggtgtc cttgaccacg ttgcacattt
     1021 cggccagcgt cagttcgaca tcgtcgaccg acacgccggg cttcaggcgg acttcgttga
     1081 acagcatctt ggcgccatag ggaatcgtga ttggactgaa catgaccggg ctcctcaaag
     1141 ggttgttaat cagaaagtgc ttatatgact gcacaccccc agctggagtt cccgagtgat
     1201 ttactcggct taatggccgg gcggggctca gccccaaacc gaacgatggc agtcgcaaga
     1261 tgacgacctg cgggccccgc gagatcagca cgccgttccg gccgattccg gtcgccgtgc
     1321 ccgagggcat gaagcccaac gagttcttca acagcagcga gaatctggcc gatctgctcc
     1381 acaacaacgg tctgctgcaa acaccggaga acctgttgct ctatcgcaag gcgctgggcc
     1441 acagcgtcga gttcgactgt tcgatcctct acaacagctc gcgcacgatt ctcgacccgc
     1501 ttggccgccc ggtccgtcgc acccagctac cggaaccggt tcgccgtgtc tggaaccgca
     1561 tgaatacgat cgtcatcgac gagatgctgg caagctaccc ggaccccgaa cgcgcgctgg
     1621 tgcttgccgg cgaggccagc ctcgacgcca cctggccgct gacatcgccc ggcgttccga
     1681 gcatccggat gctgcacaac catttcatcg tctttccgat ggcggcactg cgcgccgcga
     1741 agcacgccga tgtcgacgac ccgaacctca ccgatggcgg ccagcacagc ctgttccagg
     1801 cccatatgcg cgaggtctac cgccgcttct tcgacgaggc actaacgttg accacgctcc
     1861 ggttgctgcc gacgtcggaa tcgacgctgg ccctgaccgg ctatccgcag ggattgcccg
     1921 gttgggaaat cgtcggcggc gcggcgacga tcgcccgccc agatttctgg agcgactacg
     1981 acgccatcct caagggtttt ctcgatttct accgcagctt tttcgggcag gtctcgacgc
     2041 gcaacgccga gcccccggcc ggcctttatt tcccaggggc cgtcgaagcg atcctgctcg
     2101 ccaacaacga cttcctcgcc accgcgaaga aggttcgcga gcgctgcttg gcagacgccc
     2161 gctacgccaa tgcgctgcgc tggcaaccgg cgttcaagca gttgatctac cgtgacgagc
     2221 gcggccggct cgtcgtcacc atcagccaga actcgatcgg caacgcgatc accgagctgc
     2281 tcggcgtcgt cgtccgccgc gtccctgatg ccgaggccta cgcccaacac gaacctgcgc
     2341 tgctcgagcg gctgctcgcc ctgcgaacca agctgatcga cgccgatatc ggccacggga
     2401 ttgccaccgg acagtgggga gccgtgtagt ctgttcgttc gcaacgacct gccggatcga
     2461 tgaccgagtc cattcccgcc gccgcgccag ctgccgatgc ctggaatccc ggtatcggtc
     2521 cgggcattcc gccccaattt cggcgcctgg aaaccatttt tcgcccggaa tgcagcagca
     2581 gcagcccggg cgaaatcgcc gagctggaat cgctgaccgg cctgccgagc gaggagttga
     2641 cggcgtttcg gcccgagcgt ctggccttgc acgaagtcat cgtccgcgtt accgccgacc
     2701 tggccgttgc cgaaggcgag atcgaggaga acttcggcca gaatttccgc gccattgcac
     2761 gcctgctact cgccgaagcc gtcgagccga acatcgcgcg cctcgctgcc gactggaaag
     2821 cctggcgtga cggcgccgcg gccgaaatcg ccagaattct cgaaaccgca ctgagtgcaa
     2881 cgccggccga gcccaagagg cgccgatggc tcgaccggct ctttgcctgg aagcacagcg
     2941 ccgccggccc gtcgtggcca cccgacgagg ctgacgtggt ggccggcttc cgcagcacgg
     3001 gcctcgccgc cgagcatccg gaaacgcggg cgatctatcg cagcctctac cgcgtgctcg
     3061 gcgcaatcca gatccgccgc ggtcgcattg gcggcgacac ggcgctgctc gcccgactgg
     3121 tgacggatca cgtcgccaac gactacggcg gtcagttgct cggcacctgg ctgtcgcctg
     3181 cattcgctgc gacgatcgaa cggcatggcc tcacgcgtgt gccgccacgc gatgcgccgg
     3241 tcctgatcag cctcaagggc gcttcggcgg ccggcaaaag ctcgctgcgg ccgatgatca
     3301 aacgcctgct ctacgaggaa ggaatcgagc ccgacggcta cgcgacaatc agccccgacg
     3361 tctggcgccg cctgctgctg gactacgaag cgctcggcga cgcctacaag tacgccggcc
     3421 accttaccag ccgcgaactg atggtcatcg acggcaaact cgaccgctat atccggcgca
     3481 aggcaacgat cgaccatgcg attccgcacc tgctggtcga ccgcttccgt ttcgacagtt
     3541 tcgagagcga gaagatcggt cgcgtccttc acgagaccta tgcccgccac gtcgatacgc
     3601 tctacatgta tttcgtcatc actccgcccg aggagaccgt gctgcgcggc tggcaacgcg
     3661 cgctcgaacg gggccgctac aaggcggtcg aagacttcct cgggcacagc gtcgaggcct
     3721 attcggggat gcccaaggtc ttcttcaaat gggtcgccca tgaacgcccg ctctatcgtt
     3781 atagcttcct cgacaaccgc gttccgaagg gaagctttcc gcgaaccatt gccttcggca
     3841 cccagcggga aatgaccatc tgcgatccgc ttggattagt cgatatcgcg cgttaccagc
     3901 gaatcgacgt caatgcggaa accgccgacg cggtgtaccc cgccaccgag gatttctcgg
     3961 tcgaagccaa tctgggcttc ctcgacgagt gcctgcagca acttccgctg attcgcttcg
     4021 ccgaaacacc cgacgcgccg ccctatgccc ttcgtcagag tgggcaattc gccatcctcg
     4081 aacccgagcg cttcgccgcg ctgcggcgcg tgccggacct cgaccgcgtc tttgcccaca
     4141 tcctcggtga atgaggggat tcggcgattt cacggcaccg tgaaatcgcg tataaacggt
     4201 ggcaatccat tcctctgccg acccacgcaa actggatgaa gctctctcgt ccgctcctct
     4261 tggcagtctc gatctggacc cttctggtcg gcgcttcctt tgcgtggaac acgctgcgcg
     4321 agtacgacga ggtcttcgaa tcggcccgct ccgacgcccg gaccagcctc gaccaggacc
     4381 tgagctttcg ccgttggggc acccgccacg gcggcgttta tgttccgatc gataagcatc
     4441 aacagcccaa cccctggctg tcgcatatcc ccgaacgtga cctgaccacg accgacggtc
     4501 gccgactcac gctggtcaac ccggcgatga tgatgcggga agtcatggag gactacggcg
     4561 tcgaattcgg tatcgagggg cggatcaccg gactcaaata ccttaacccc gccaatgccc
     4621 cggacgaatg ggagcgcgca cagctcgaag cattcgcccg cggcgaacgc aaggaaacaa
     4681 ccgagatcgt gccgctgaag ggtgcgcggc atctgcgcca cctgcgtgca atgtggatgg
     4741 aaccgggttg cgagaagtgt catgccgttc tcggctacaa gaccggcgac ctgcgcggcg
     4801 ccaccggcgt caacctgccg ctggcgccct acgaggcacg tattctcggc gacctgatca
     4861 atctcggcgt gactcacggc ctgatctggc tggtcggcct cgccggcatt ggctgggtga
     4921 cgcgcgattt ctcgcaccgg caggcggcgc tgcgtgccag tgaaaagcgt tttcgcgacc
     4981 tcttcgacaa ttcgccggac ccgtgctggc tgatccagga agacgatgtc gtcgactgca
     5041 atcgtgcggc cgccgagatg ctcggctatc gctcgatcga tgaggttctg caaaacgtcg
     5101 accgcctgtc gcccgagcgc caaccggacg gccagctttc agcagtgaag tcagcggcga
     5161 tggccgcact cgcccgccag aacggcgtgc atcgctttga atgggaacag cggcgggcag
     5221 acggttcctg ttt
//
LOCUS       DATDAA010000086         2946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_76285,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000086 DATDAA010000000
VERSION     DATDAA010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76285"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1603)
                     /gene="fumC"
                     /locus_tag="VIS73_02835"
     CDS             complement(173..1603)
                     /gene="fumC"
                     /locus_tag="VIS73_02835"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY5762120.1"
                     /translation="MTATRREEDSFGAVEVPAGRLWGAQTQRSLQHFSISDERLPAAL
                     IAALGMAKAACARVNGALGRLPPEKAAAIAAAAEEVAAGDHADEFPLRVWQTGSGTQT
                     NMNVNEVIANRASQLLGGALGSARCVHANDDVNLGQSSNDVFPTAMHVAAALAIHRAG
                     SAAAGPRPPVLPTVQALQAALAGKAAAFASIVKVGRTHLQDATPLTLGQEFSGYAAQL
                     EQAGAQIAANLPLLYPLAIGGTAVGTGVNTHPEFGDRVAADLKAQTGIPFTSAANKFA
                     ALAAHDALVSAHGALKTLAVALNKIANDLRWLASGPRSGLGEIILPANEPGSSIMPGK
                     VNPTQCEAMTMLCVQVIANDVAIGIAGASGNFELNVYKPLIAHAFLQSTRLLAHGMDG
                     FTRHCVLGIAANCERIDELMQRSLMLVTALVPHIGYDKAAEIAHRAQGDGSTLRAAAI
                     ALGHVSGEDYDRWVVPAAMTGPIADK"
     gene            complement(1656..2834)
                     /locus_tag="VIS73_02840"
     CDS             complement(1656..2834)
                     /locus_tag="VIS73_02840"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY5762121.1"
                     /translation="MQLSLLDLPWWGLVAVTLAMTHVTIAAVTIYLHRHQAHHALTLH
                     PLVSHFFRFWLWLTTGMVTREWVAIHRKHHAHCDAEGDPHSPQRYGLPRVLWQGSELY
                     RDEAANAETLSRYGKGTPDDWVERHLYTPHSALGVGILAVADVVLFGPIGLTIWAVQM
                     LWIPVFAAGVINGVGHYWGYRSFATRDASRNIVPWGILIGGEELHNNHHAYAASARLS
                     NRWWEFDAGWLYIRLLENVGLARVNRIAPQLRRRSALAQCDADTLQAICTHRFAVLAQ
                     FARALKRTAVSELNALRDRGAKEAKSLSLRRALRRRLQRDDIVLPAPEQAALEQAIGA
                     STVLQTIFAMRQDLATLWSRTNESKEELVRRLERWCQSAEASGIAALRDFSRSLRGFA
                     "
BASE COUNT          474 a          944 c         1042 g          486 t
ORIGIN      
        1 cggtgggaga caggggggtg ccgaggtgca tgaacgactc cgcttgcgta ggggatggcc
       61 gatgctaacg cagccagaac agtccggcac ggccgccgag cttgcgcagt ggacagtcga
      121 aaaaatcggg cggcgggccg ccgtgcgtct cgccaccgcc tggaagcccg atctacttgt
      181 cggctatcgg accggtcatc gccgccggca cgacccagcg atcgtagtct tcgccgctga
      241 catggccgag ggcgatcgcc gccgcccgca aagtgctgcc gtcgccttgt gcccggtggg
      301 cgatctcggc cgccttgtcg tagccgatgt gcgggaccag tgccgtcacc agcatcagcg
      361 agcgctgcat cagctcgtcg atccgctcgc aattggctgc tatgccgagc acgcaatgcc
      421 gggtaaatcc atccatgccg tgggcgagca ggcgcgtgct ttgcaggaag gcgtgggcga
      481 tcagcggctt gtagacgttg agttcgaaat tcccggaagc gccggcgatg ccgatcgcaa
      541 catcattggc gatgacttgc acgcagagca tggtcatcgc ctcgcactgc gtcggattga
      601 ccttgccggg catgatcgaa ctgccgggct cgttggctgg caagatgatc tcgcccagcc
      661 ccgagcgcgg cccagaggcc agccagcgca ggtcgttggc gatcttgttc agggcgacgg
      721 ccagcgtctt cagcgcgccg tgcgcgctca ccagtgcgtc gtgcgccgcc agcgccgcaa
      781 acttgtttgc cgcactggta aacgggattc cggtctgcgc cttgagatcg gccgcgacgc
      841 ggtcgccgaa ctcgggatgg gtgttgacgc cagtgccgac tgccgtgccg ccgatcgcca
      901 gcggatagag cagcggcagg ttcgcggcga tttgcgcgcc ggcctgttca agctgcgccg
      961 cgtaaccgga gaactcctgg cccaaggtca gcggcgtggc gtcctgcagg tgggtgcggc
     1021 cgaccttgac gatagaggcg aaggcggcgg ccttgccggc aagggccgct tgcagcgcct
     1081 gtaccgtcgg aagcacgggt ggccgcgggc cagcagccgc gcttccggcc cggtgaatgg
     1141 cgagtgcggc ggcgacgtgc atcgccgtcg gaaagacatc gttcgacgac tggccgagat
     1201 tgacgtcgtc gttggcgtgg acgcagcggg cgctgcccag cgcgccgccg agcagctgcg
     1261 aggcgcggtt ggcgatcacc tcgttgacat tcatgttggt ttgcgtgccc gagccggtct
     1321 gccagacgcg cagcgggaat tcgtcggcat ggtcgccggc ggcgacttcc tcggcggcgg
     1381 cggcgatcgc tgccgccttt tccggcggca gccggccaag cgcgccattg acgcgtgcgc
     1441 aggcagcctt ggccattccc agcgcggcga tcagtgccgc cggcaggcgc tcgtcggaaa
     1501 tcgaaaaatg ctgcagggag cgctgggtct gcgcgcccca cagacgcccg gcgggcactt
     1561 cgacggcgcc gaaagagtct tcttcgcggc gggtcgcggt cacgttgatg gacctcttgt
     1621 cacctttctg ctcccgggcg gcggccaccg gccgctcagg cgaagccgcg caggctccgc
     1681 gagaagtcgc gcagcgccgc gatgccgctg gcctcggccg attggcacca gcgctctagc
     1741 cggcggacca gttcttcctt ggactcgttg gtccggctcc acagcgttgc caggtcctga
     1801 cgcatggcga agatcgtctg cagcaccgta ctggcgccga tcgcctgttc gagtgccgcc
     1861 tgctcgggtg ccggcaatac gatgtcgtcg cgctgcaaac gacggcgcag ggcgcgtcgg
     1921 agcgacaggc tcttggcctc cttcgcgccg cgatcgcgca aggcgttcaa ttcgctgacc
     1981 gcggtccgtt tgagcgcgcg ggcgaactgc gcgagtacgg cgaagcggtg cgtgcagatc
     2041 gcctgcaggg tatcggcatc gcactgcgcg agagccgatc gccggcgcag ttgcggcgcg
     2101 atgcgattga cgcgggcgag cccgacgttc tcaagcaggc ggatgtagag ccagccggcg
     2161 tcgaactccc accagcggtt cgacaagcgc gccgacgcgg cgtaggcatg gtggttgttg
     2221 tgcagctctt cgccgccgat caggattccc cagggcacga tgttgcgcga ggcatcgcgg
     2281 gtggcgaagc tgcggtaacc ccagtagtgg ccgacgccat tgatcacgcc ggcggcgaac
     2341 accggaatcc agagcatctg caccgcccag atcgtcaacc cgatcgggcc gaacagcacg
     2401 acatcggcaa ccgcaagaat gccgaccccg agcgccgagt gcggcgtgta gaggtggcgt
     2461 tcgacccagt cgtcgggcgt gcccttgccg tagcgggaaa gcgtctcggc gttggcggcc
     2521 tcgtcgcggt acagctcgga accctgccag agcacgcgcg gcagcccgta gcgttgcggg
     2581 ctgtgcgggt cgccttcggc gtcgcagtgc gcgtggtgct ttcgatggat cgccacccat
     2641 tcacgggtga ccatgccggt tgtcagccac agccagaagc ggaagaaatg gctgaccagc
     2701 gggtgcagcg tcagcgcgtg gtgggcctga tggcgatgca ggtaaatggt gaccgcggca
     2761 atcgtgacgt gggtcattgc cagcgtcacg gcgacgaggc cccaccaggg gagatcgagt
     2821 agcgacagtt gcatggtgtg tccttttcag cgtggacacc gcaaaacgcc gcatccgatg
     2881 cgatttcgac tgtgccaggc gagaaaaaat ccccagcggc ggaaaacgtg atcggcgctg
     2941 gccttg
//
LOCUS       DATDAA010000087         8175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_216385,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000087 DATDAA010000000
VERSION     DATDAA010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216385"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1026
                     /locus_tag="VIS73_02845"
     CDS             220..1026
                     /locus_tag="VIS73_02845"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEY5762122.1"
                     /translation="MGQTTEKSTNEPARGARYTEETILERRDWAPRLFSFRTTRHPGF
                     RFTAGQFARIGLPGADGKPVWRAYSIVSAPYDTHLEFYSIVVPEGEFTPKLAERHEGD
                     AVLIEKTNYGFLTPDRFAPGRDLWMLSTGTGLAPFLSILAEPQPWQDFENLILVHSVR
                     HANELAYQREIGELRDHPLVGEHSHRLRYVPVVTREPVEGALGERITTLIASGELGRH
                     TGLELSTDCSRFMVCGNPEMVDDVRALLQERGFRVSRRSNPGHMVFENYW"
     gene            1067..1477
                     /locus_tag="VIS73_02850"
     CDS             1067..1477
                     /locus_tag="VIS73_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY5762123.1"
                     /translation="MDTLITELLDKARTIAVVGLSDKPHRDSYRVAAYLQDAGYRIIP
                     VNPRCQSILGERCYPDLASIPDPVDIVDVFRRGEETPAVARAAVAAGAGALWLQLGIS
                     NETTRQIAGEAGMPLVMDRCILIEHRRRDATTSA"
     gene            complement(1495..2238)
                     /gene="rlmB"
                     /locus_tag="VIS73_02855"
     CDS             complement(1495..2238)
                     /gene="rlmB"
                     /locus_tag="VIS73_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEY5762124.1"
                     /translation="MAETRFIFGFHAVLAALRHSAEKVHEIFIDESRQDVRGRDLIKR
                     ANDLKVRIVQADHKRLDGMAPPARHQGVVARVESAPRVSSLDDLLDGLSEPPLLLVLD
                     GVQDPHNLGACLRVADGVGAHAVIAPKDRACGLNATVSKVASGAAESVPFVTVTNLAR
                     TLRELKERDIWIIGTSDQGSTDLYAAEWPSACAWVLGAEGEGMRRLTGEQCDQLVRIP
                     MHGSVESLNVSVASAVCLYEARRRRLPTA"
     gene            complement(2302..2874)
                     /locus_tag="VIS73_02860"
     CDS             complement(2302..2874)
                     /locus_tag="VIS73_02860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY5762125.1"
                     /translation="MPKILVFSGSIRRDSVNRRLAQVAAAAVSAAGGEVTLLDLREYP
                     LPIYDGDLEDAEGLPANAVALKTIFKAHAGLFIVSPEYNGFFPPLLKNTIDWLSRPHG
                     AESGLAPYAGKVAAIAAASPGGLGGIRCLPLLRQQLSNLSVTVLPQQLALPSADRAFD
                     ESGALRDAGQASSLQRIAAELLRVTGLLAG"
     gene            complement(2874..3578)
                     /locus_tag="VIS73_02865"
     CDS             complement(2874..3578)
                     /locus_tag="VIS73_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY5762126.1"
                     /translation="MIEKRPASSRGHANHGWLDSWHSFSFADYHDPAQMGWGTLRVIN
                     DDTVEPAGGFPSHGHRDMEIVSVVLEGALEHRDSMGNGTVIKAGEVQRMSAGSGVTHS
                     EFNPSATERSRFLQIWIRPRAAGGAPGYEQQAFRPEETPGRWRVLASADGRDDSLTIN
                     QDASILAAALLGGEAIAHDPGRGRKQYVHVVRGEIDVNNIRLGAGDGAKIAAEAALAF
                     AAHADSIFLLFDLTES"
     gene            complement(3658..5118)
                     /locus_tag="VIS73_02870"
     CDS             complement(3658..5118)
                     /locus_tag="VIS73_02870"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY5762127.1"
                     /translation="MENADSKRTTASRGFLPYASIAVLAILLVTVLAAGYLISERRAA
                     LRDGYLIAQQSAGFSALATEGTLDSARQLLRSMAVIAGEAKREGGKAAPAVRRTLLDW
                     QRADPYLMDLLILDSAGRIIHWTGPGTPPPVDDRPYFRVHAENNRSGLHLGRPLLSKV
                     HQGQWFCALSEAIRAEDGKLNYVVVAIIDVRLLRDRLHVGAAVPGTTQALLSEDGVVY
                     VRTPEHEKYVGKSVSRVAELAMLSDLVPSTTIETVSQLDGRARILAFQKIRGFPLVAA
                     GTIETDAVYRSWWRYAFAGTLLWLVIVIGIVWIVLRLHRERKVLAELASIDSLTGTLN
                     RRSILDTATNLERSQSFAGALSLLMIDADHFKSINDRFGHAVGDDVLRRLSEVLRRNV
                     RTNDIVGRYGGEEFLVLMPDTGEPGALRVAEKLRAAVEAQITRPVPLTISVGVATTRE
                     GALSLDRTLAHADAALYAAKAAGRNCVRADGDHTAS"
     gene            complement(5165..5524)
                     /locus_tag="VIS73_02875"
     CDS             complement(5165..5524)
                     /locus_tag="VIS73_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEY5762128.1"
                     /translation="MTNDEIRDLQQRWVAFRDARNWRQYHSLRNLITTVNLEAKELLE
                     LTLWRTDSEVDALADSPETRTALADECADVLLGLMLVADKAGIDLFRAACEKIEKNEL
                     KYPVDKSYGSRKKYTEL"
     gene            complement(5531..6220)
                     /gene="lolD"
                     /locus_tag="VIS73_02880"
     CDS             complement(5531..6220)
                     /gene="lolD"
                     /locus_tag="VIS73_02880"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEY5762129.1"
                     /translation="MNEANGNVLACEALTKIYRSGEIEVSVLTGIDLRVSRGESVAIV
                     GASGSGKSTLLHLLGGLDSPSAGIVRWQGEDIAGFSEGQRSALRNRDLGFVYQFHHLL
                     PEFSALDNVAMPLLIRRLERGEALARAADMLARVGLGERVAHTPAELSGGERQRVAIA
                     RALVTGPACVLADEPTGNLDSRTADTVFSVLVELQASLGMALILVTHDTELAGRTHRR
                     LTLRDGRLETG"
     gene            complement(6213..7460)
                     /locus_tag="VIS73_02885"
     CDS             complement(6213..7460)
                     /locus_tag="VIS73_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY5762130.1"
                     /translation="MALPFELWVGLRYTRAKRRNHFISFISLISMGGVALGVAALIVV
                     LSVMNGFQEELRTRILGVASHVQVSGIGNEVPEWEEVAAQAGQHPRVRATAPYVQGQG
                     MLSFEQAVRGVQVRGILPEREEAVAGFREFMKRGRWDDLQPGEFGIFLGTDLASALGA
                     RLGDKVALIVPQGNVTIAGVAPRLKAFRVVGIFEVGMYEYDSGLALIHLADAQKLYRM
                     GERVSGVRLKLDDLFAAPKVSRELLRTLDDHLMVSDWTRAHANFFRAVQIEKNMMFII
                     LSLIVAVAAFNIVSTLVMAVTDKQADIAILRTLGASPRSILWIFMVQGTLIGIVGLGL
                     GVTLGLSLAFNIDVVVPAIERLLGTQFLAKDVYYISELPSQVQWSDVFTVSGVAFALS
                     LLATIYPSIRAANTNPAEALRYE"
     gene            7569..>8175
                     /locus_tag="VIS73_02890"
     CDS             7569..>8175
                     /locus_tag="VIS73_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762131.1"
                     /translation="MRLSLLVPDLLWGRTDNTPLFETLARLPGAGLLAARTPTALAYH
                     PAEAILATSCGLGDSVGGVDLAALRHHGEPGQPVELERGKANRILCADPVHFCFHQEF
                     LIAADDSQIGLSADEADQLIDALNKHFAERARFNAATPSRWYVELAASPVGSWPPLAE
                     IVGRSLAPRLFEPAALQQLGSEAQMLLFAHPVNRGREDAGRP"
BASE COUNT         1504 a         2777 c         2531 g         1363 t
ORIGIN      
        1 ggtaccgcac cgacagcgtc accgaccgcg ccagcgacac catcaacgag cgcgccggtt
       61 gccacgccaa cgcccagccc gacgcccact gttcccgtca gcacgcaccc gcacggcgga
      121 cctcccggcc tgaacaagta gcgttgcgcc cagaacctct aggaactgcc cggacattta
      181 cctgactaaa acagtaaggt ttaacgcgag ggcagtacga tggggcaaac gactgaaaag
      241 tccactaacg aaccagcgcg cggcgcgcgc tacaccgagg agaccatcct cgagcggcgc
      301 gactgggcgc ctcgcctgtt ttccttcagg accacccgcc acccggggtt tcggttcacc
      361 gccgggcagt tcgcccggat cggtttgccg ggagccgacg gcaagcccgt ctggcgggcc
      421 tattcgattg tctcggcacc ctacgacacg catcttgagt tttattcgat cgtcgttccc
      481 gagggcgagt tcacgccgaa actcgccgag cgccacgagg gcgacgccgt cctcatcgaa
      541 aagaccaact acgggtttct cacacccgat cgtttcgcac cgggccgcga cctgtggatg
      601 ctatcgaccg gaaccgggct cgcgccattc ctctccatcc tcgccgagcc gcagccgtgg
      661 caggacttcg agaacctcat cctcgtgcac agtgttcgcc acgccaacga gcttgcctat
      721 caacgggaaa tcggcgaact gcgagaccac ccgctggtcg gcgagcacag ccaccgtctg
      781 cgctacgtgc cggtggtcac gcgcgaaccg gtcgagggag cgctgggcga gcggattacc
      841 acgctgatcg ccagtggcga gctcggccgc cataccggtc tcgaactcag caccgactgc
      901 tcgcgcttca tggtctgcgg caatccggaa atggttgatg acgtccgcgc cctgcttcag
      961 gaacgaggct ttcgcgtcag ccgccgaagc aatccggggc acatggtttt cgagaactac
     1021 tggtgatagg ctcgacgccc gcacctctga atcgggcacg ccgacaatgg atacgctgat
     1081 caccgaactg ctggacaagg cacggacaat cgccgtggtc ggcctctccg acaagcccca
     1141 ccgggactcc taccgggttg ctgcctacct ccaggacgcc ggctatcgca tcatcccggt
     1201 caatccccgc tgccagtcga tactcggcga gcgctgctac cccgacttgg cgagcattcc
     1261 cgacccggtc gacatcgtcg acgtcttccg ccgcggcgag gaaacacccg cggtcgcccg
     1321 cgcggccgtc gccgccggcg ccggtgcgtt gtggctgcag ctgggaatca gcaacgagac
     1381 aacccggcag attgccggcg aagccggaat gccgctggtg atggaccgct gcatcctgat
     1441 cgagcatcgc cgccgcgacg ccaccactag cgcttaggct cgatcgccgt cggcttaggc
     1501 cgtgggcagg cgccgccgcc gggcctcgta gaggcagacc gccgaggcga ccgaaacgtt
     1561 gagactctca acgctgccgt gcatcgggat cctcaccagc tggtcgcact gctcgccggt
     1621 cagacggcgc atcccctcgc cttcggcacc cagcacccag gcacaggcgc tcggccactc
     1681 ggccgcatag aggtcggtgc tgccctggtc actggtgccg atgatccaga tgtcgcgttc
     1741 cttcaattcg cgcagcgtac gcgcgagatt ggtgacggtg acaaagggca ccgattcggc
     1801 agcgccgctg gcaaccttgc tgaccgtcgc gttaagtccg caagcgcgat ccttgggcgc
     1861 gatcaccgca tgcgcaccga cgccgtcggc gacacgcagg caggcgccga ggttgtgcgg
     1921 gtcctgcacg ccatcgagaa ccagcagcag cggcggctcg ctgagtccgt cgagcaggtc
     1981 atcgaggctg ctgacgcgcg gcgccgattc gacccgggca acgacgccct gatgccgagc
     2041 cggcggcgcc atgccgtcaa ggcgcttgtg gtcggcctgg acgatgcgga ccttgaggtc
     2101 gttggcgcgc ttaatcaggt cgcggccgcg aacgtcttgc cgcgactcgt cgatgaatat
     2161 ctcgtggacc ttctcggcac tgtgccgcag ggccgccaga acagcgtgaa aaccgaagat
     2221 gaagcgcgtt tccgccatga tgttctcctg ctgccgtcgc gaaagacggc ggaggataca
     2281 ggatcgctgt ctgcgcctta gtcagcccgc cagcaagccg gtaacccgca gcaattcggc
     2341 ggcgatgcgc tgcaggctgg acgcctgccc ggcatcgcgc agggcgcccg actcatcgaa
     2401 agcccggtcg gcagacggca aggccagctg ctgcggcaag accgtcaccg acaggttgct
     2461 caactgttgg cgcaacaagg gcaggcagcg gattccgccc agcccgccag gcgatgccgc
     2521 cgcaatcgcc gcgaccttgc cggcgtaggg cgcgaggccg ctttccgccc catgcgggcg
     2581 cgacagccag tcgatggtgt tcttcagcag cggtggaaag aaaccgttgt actcgggcga
     2641 cacgatgaac aggccggcat gcgccttgaa aatcgttttc agcgccacgg catttgccgg
     2701 caggccctcg gcatcttcga gatcgccgtc gtagatcggc agcgggtatt cgcgcaggtc
     2761 gagcagcgtc acctcgccac cggcggcgct aaccgcagcg gcagcgactt gggccagtcg
     2821 gcggttcacc gagtcgcggc gaatgctgcc ggaaaaaacc aggatcttgg gcatcaggac
     2881 tccgtcaagt caaaaagcag gaatatcgaa tcggcgtgcg ccgcaaacgc cagcgcggcc
     2941 tcggcagcaa tcttcgcacc atcgcctgca cccagccgaa tattgttcac atcgatctcg
     3001 ccgcgaacga catggacgta ttgctttcgt ccgcgccccg gatcatgcgc gatggcctcg
     3061 ccgcccaaca gtgctgccgc gagaatgctc gcgtcctgat tgatcgtcag gctgtcgtct
     3121 cgtccgtcgg ccgaggcgag gacacgccag cgccccgggg tttcctccgg gcgaaacgcc
     3181 tgctgctcgt aaccgggcgc gccgccagcc gcgcgcgggc gaatccaaat ctgcaggaag
     3241 cggctgcgct cagtcgccga aggattgaat tcgctgtgcg taacgccgct tccggcgctc
     3301 atgcgctgga cctcgccggc cttgatgacc gtcccgttgc ccatgctgtc ccggtgctca
     3361 agcgcgcctt cgaggaccac gctgacgatc tccatgtcgc ggtgcccgtg acttggaaat
     3421 ccaccggcgg gttcgaccgt gtcgtcgttg atgacgcgca gcgttcccca gcccatctgc
     3481 gccggatcgt gatagtcggc aaacgaaaag ctgtgccagc tatcgagcca gccgtgattg
     3541 gcgtggccgc gcgagcttgc cggtcttttt tcgatcattt ctactggacc cctggtcgca
     3601 tgacagcccg attgactgtc ggcggcagcc gtggttcccg cgctggccag cgcgtactca
     3661 gcttgccgtg tgatcgccgt cggcgcgcac gcagtttcgc ccggccgcct tggccgcgta
     3721 gagcgccgcg tcggcgtgcg ccagcgtgcg gtcgaggctc aacgcgccct cgcgcgtggt
     3781 cgcgacacca acgctgatgg tgagcggcac gggccgggta atttgcgcct cgaccgccgc
     3841 gcgcagcttt tcagcgacac gcaacgcgcc aggctcgccg gtatcgggca tcagcacaag
     3901 gaattcctcg ccgccgtagc gaccgacgat gtcgttggta cgaacgttac gccgcaggac
     3961 ttcggagagt cgacgcagca cgtcgtcgcc aacggcgtgg ccgaagcggt cgttgatcga
     4021 cttgaaatga tcggcatcga tcatcaacag cgacaaggca ccggcaaagc tctggctgcg
     4081 ctcgagattc gtcgccgtgt cgaggatgct ccgccggttc agtgtgccgg tcagcgagtc
     4141 gatgctggcc aattccgcga ggaccttccg ctcacgatgc aaccgcagga caatccaaac
     4201 gatgccgatg acgatcacca gccatagcaa cgtaccggcg aaggcatagc gccaccacga
     4261 tcgatagacc gcgtcggttt cgatcgtgcc ggctgcaacc aggggaaacc ctcttatctt
     4321 ctggaacgcc agaatccgtg cccggccatc gagctgcgag accgtttcaa tggtggtgct
     4381 aggcaccaga tccgacagca tggccagctc ggcaacacgg ctgacgctct tgccaacata
     4441 tttctcatgc tcgggagtgc gaacgtagac cacgccgtcc tcgctgagca aggcctgcgt
     4501 ggttcccggc actgccgccc cgacgtgcaa acgatcgcgt agcagccgca cgtctatgat
     4561 cgccaccacc acgtaattga gtttgccgtc ctcggcgcga atcgcctcgc tgagcgcgca
     4621 aaaccactgt ccttgatgaa ccttggaaag cagcgggcgg cccagatgga gtccgctgcg
     4681 attgttttcc gcgtgaaccc ggaaatacgg gcggtcatcg accggcggcg gcgtgccagg
     4741 cccggtccag tggatgattc gccccgctga atccaagatc agcaggtcca tcaggtaggg
     4801 gtcggccctt tgccagtcga gcagcgtccg gcgtaccgcg ggggcggctt tccctccctc
     4861 gcgcttcgcc tcaccagcga tcaccgccat tgaacgcagt aattggcggg cgctgtcgag
     4921 ggttccctcg gtcgccaggg ccgaaaaacc ggcactttgt tgagcgatca agtagccatc
     4981 gcgcaaggca gcgcgtcgct cactgatcag gtagcccgcg gcaagcacgg tgaccagcag
     5041 tatggccagg acggcgatgc tggcgtaggg cagaaagcca cgactggcag tcgtgcgctt
     5101 cgagtccgcg ttctccatta cgacgaatct ccagtaggca gcgcttgccg gaaccggtcg
     5161 cgcactacag ttcggtgtat ttcttgcgcg acccgtagct cttgtcgacc ggatacttca
     5221 actcgttctt ctcgatcttt tcgcacgcag cacgaaacaa atcaataccg gccttatcgg
     5281 caacgagcat cagtcccaac agaacgtcgg cgcattcatc ggcgagcgcc gtgcgtgttt
     5341 cgggcgaatc ggccagcgca tcgacctcgg aatcggtccg ccagagcgtc agctcaagca
     5401 gttccttggc ctcgaggttt accgtggtga tcaggttgcg cagcgagtgg tactgacgcc
     5461 agttgcgtgc gtcgcgaaag gcaacccaac gctgctggag atcgcggatc tcgtcgttag
     5521 tcatcgcacc tcagcccgtt tccagtcgcc catcgcgcag cgtcaaccgg cgatgagtcc
     5581 ggcccgccaa ctccgtatcg tgggtaacga ggatcagcgc catcccgagg ctcgcctgca
     5641 actcgacgag caccgagaac accgtgtcag cggtccggct gtcgaggttc ccggtcggct
     5701 catcggcgag tacgcaggcc ggccccgtga ccagcgcccg cgcgatcgcg acgcgctggc
     5761 gctcgccacc agacagctcg gccggcgtgt gagcgacgcg ttcgccgagc ccgacccggg
     5821 ccagcatgtc ggctgcccgc gccagtgctt cgccgcgctc aaggcgacgg atcaacagcg
     5881 gcatcgcaac gttgtcgagc gccgagaact ccggcaacag gtggtggaac tggtagacga
     5941 aaccgagatc gcggttgcgc agcgccgagc gttgcccctc ggaaaacccg gcgatgtctt
     6001 cgccctgcca gcgcacaatg ccagcgcttg gcgaatcgag gccgccgagc aggtgcagca
     6061 gcgtgctctt gcccgaaccc gaggcgccaa cgatggcgac gctttcgcca cgcgaaacac
     6121 gcaagtcgat gccggtcagc accgaaacct caatctcgcc acttcggtag atcttggtca
     6181 gcgcctcgca ggcgagcacg ttgccgttcg cctcattcat agcgcagggc ctcggcggga
     6241 ttggtattcg cggcgcggat gcttggataa atcgtcgcca gcagcgacag cgcgaaggct
     6301 acgccggaca ccgtaaacac gtcgctccat tgcacctgcg agggaagctc gctgatgtaa
     6361 tacacgtcct tggcgaggaa ctgcgtgccg agcaagcgct cgatcgccgg caccacgacg
     6421 tcaatgttga aggccagcga cagcccgagc gtgacgccga ggccaagacc gacgatcccg
     6481 atcaaggtac cctgaaccat gaatatccac aggatgctgc gcgggctggc gcccagcgtg
     6541 cgcaggatgg cgatgtcggc ctgcttgtcg gtgacggcca tgaccagcgt cgagacgatg
     6601 ttgaaggcag cgacggcgac gatcagcgaa aggatgatga acatcatgtt cttttcgatc
     6661 tgcacggcgc ggaagaagtt ggcgtgggcg cgcgtccagt cggaaaccat caggtgatca
     6721 tcgagcgtcc gcagcaattc ccgcgagacc ttcggcgcgg caaacaggtc gtcgagcttg
     6781 aggcggacgc cgctgacccg ctcacccatc cggtagagtt tctgggcgtc ggcgaggtgg
     6841 atcagcgcca ggccgctgtc gtactcgtac atgccgacct cgaagatgcc gacgacgcga
     6901 aaggccttca ggcgtggcgc gacgccggca atcgtgacgt tgccctgggg aacgatcagc
     6961 gccaccttgt cgcccaagcg cgcaccgagc gcactggcca gatcggtgcc aagaaagatg
     7021 ccgaactcgc ccggctgcag gtcatcccag cgaccgcgct tcatgaactc gcgaaagccg
     7081 gcgacggctt cttcgcgctc gggaagaatg ccgcggacct ggacgccgcg aaccgcctgc
     7141 tcgaagctca gcatgccctg cccctgtacg tagggcgccg tcgcccgcac gcgcgggtgc
     7201 tgcccggcct gcgccgcgac ctcttcccac tcgggaacct cgttgccgat gccactgacc
     7261 tgcacgtgcg aggccacgcc gagaattcgc gtccgcagtt cctcctggaa gccgttcatc
     7321 accgagagca cgacgatcag cgccgccacc ccgagcgcga caccacccat ggaaatcagc
     7381 gagatgaatg aaatgaaatg gttgcggcgt ttggcccgcg tataacgcag gccaacccat
     7441 agttcgaaag gcagggccat cgtggctttg caaggggaga acgggcgcta tggtaacctc
     7501 gccgcattcc cagccgccac tggcattcgc cggcagcatc gtgtgatccc actgattccc
     7561 agtcggcaat gcgcctgagc cttctcgtac cagacctcct ttggggccgc accgacaaca
     7621 ccccgctatt cgaaacgctg gcccggctgc ccggcgccgg cctgcttgcc gcgcgaacgc
     7681 cgactgcgct tgcctatcac ccggccgaag ccatcctcgc cacctcctgt ggcctgggcg
     7741 atagcgttgg cggtgtcgac ctggcggcgc tacgccatca cggcgagccg ggccagccag
     7801 tggaactcga gcgcgggaag gcgaaccgca tcctgtgcgc cgatcccgtg catttttgct
     7861 tccaccagga attcctgatc gccgccgacg attcacagat tggcctgagc gcggacgagg
     7921 ccgatcaact catcgacgca ctgaacaagc atttcgctga acgcgcgcgc ttcaacgccg
     7981 ccacgccgtc gcgctggtac gtcgaactcg ccgcgtcgcc ggtcggcagc tggccgccgc
     8041 ttgccgaaat cgtcgggcgc agcctggcgc cgcgactgtt cgaacccgca gcgctgcaac
     8101 aactcggctc cgaagcgcag atgctgctct tcgcgcatcc ggtcaaccga ggccgggaag
     8161 acgccggccg tccat
//
LOCUS       DATDAA010000088         3303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_216407,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000088 DATDAA010000000
VERSION     DATDAA010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216407"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VIS73_02895"
     CDS             <1..507
                     /locus_tag="VIS73_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEY5762132.1"
                     /translation="KIIRPGQAVVPGLTLQVIDGSTASLDQGGRRVDLRIGQRLPGSP
                     TAEQTAMLQANPQGHFLVTGAINDRSIRFLVDTGATMISLGASDARRLGLDATGGEEG
                     MAHTAAGVVPVRRMRLDKVSVGDIELDGVDALVHGNDLPIALLGMSFLNRTDMRREGT
                     TMTLKRRY"
     gene            517..795
                     /locus_tag="VIS73_02900"
     CDS             517..795
                     /locus_tag="VIS73_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762133.1"
                     /translation="MPHREQELTLLREELEMLMAERQLLLQVVGASAALIANLDIQRL
                     PLGAVEAADILATVVNRLPEETLRDALDAVHAGTGKMLSLAGETQSAT"
     gene            792..1397
                     /locus_tag="VIS73_02905"
     CDS             792..1397
                     /locus_tag="VIS73_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY5762134.1"
                     /translation="MSVAQLVGLARLREAVLPAPGVRVLSEPAPDESDELVPAAVLVP
                     VVTHGDPTVLLTLRTAHLRDHAGQVSFPGGRIEDDDASARHAALREAEEEIGLANHNV
                     EVIGYLPEYRTVTGFRITPVVGLVPPGVDLKCDPFEVSAVFEVPLAFVLDPQNHRNHQ
                     IHYRGRLREYTAVTYGDYLIWGATAGMLRSLFELLREPVPR"
     gene            complement(1375..2136)
                     /locus_tag="VIS73_02910"
     CDS             complement(1375..2136)
                     /locus_tag="VIS73_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762135.1"
                     /translation="MPSGTIYAKTQKGFEEMTRRTYRLPARMRSLLIMIDGHLPAGEL
                     FARAPHPEEAQQLIESLLKDGFIEPVGGIDDAGELSNAASAPLDTAASAPSPDLALVA
                     AKRYVAQAMLDALGPEADHFTVHIEAASDAGSLKAIALEYLDVIRGASDRRKADAFRD
                     GLVQLHLLGQSEGLPPDPPEAAVDLRNAKRVMVQRLVAALGPDADRFTLAAERAANAE
                     EFRALLAKYQEVVRAMAGHRKADEFVAAVSAALAP"
     gene            2335..3249
                     /locus_tag="VIS73_02915"
     CDS             2335..3249
                     /locus_tag="VIS73_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HEY5762136.1"
                     /translation="MTFLALLAALVIEQFRPLDPERFIRTPAEMAGGWLEQQANAGTY
                     RHGVIAWVLAVGVVLGMVAIVYGILCAMHPVLAWLLNVVILYWAMGFRQVSHYFTGIQ
                     KALAEGRLDDARLQLANWRNERTERMSSQDVTGLAIESALVASHRQVFAVMLCFVLLP
                     GPLGAVLYRLAEQLARVWRPSGESAFGNFAQEAFRIIDWLPQRVTAFGFAVVGDFEDA
                     VHCWRTQAETWGDAAGGVVLAAGAGALGVQLGAPINDGIEVVERPVLGLGDDPGIESL
                     QATVGLVWRALVFWMVILLALSVAGAAG"
BASE COUNT          549 a          973 c         1128 g          653 t
ORIGIN      
        1 aagattatcc gcccggggca ggccgtggta ccgggtctga cgttgcaggt catcgacggc
       61 agcaccgcga gccttgatca gggtggccgc cgtgtcgacc ttcgcatcgg acagcgcctt
      121 cccgggagtc cgaccgctga gcagacggcg atgctgcagg cgaatccgca agggcatttt
      181 ctggtaactg gtgcaatcaa cgatcgcagc atccgcttcc tcgtcgacac gggcgcgacg
      241 atgatctcgc ttggcgcgtc agatgcgcga cgactgggcc tggatgccac cggcggcgag
      301 gaaggaatgg cgcatactgc cgctggtgtc gtgccagtgc ggcgaatgcg actcgacaaa
      361 gtgtcggttg gtgacatcga actcgatggt gtcgacgcac tggtgcacgg gaatgatctg
      421 ccgattgcgc tgcttggcat gagctttctc aaccggaccg acatgcgtcg cgaaggtacg
      481 acgatgacct tgaaacggcg atattaggga gaaaagatgc cgcaccggga acaggaactc
      541 acgctgctgc gtgaagaact cgagatgttg atggccgagc ggcagttgct gttgcaggtc
      601 gtcggcgcct ccgccgcgct gatcgccaac ctcgacatcc agcgcctgcc gctcggtgcc
      661 gttgaggcgg ccgatattct ggcgaccgtg gtcaaccgcc tgcccgagga aaccctgcgt
      721 gacgcgctgg acgccgtgca cgctgggacc ggcaaaatgc tgtcgcttgc cggagagacg
      781 cagtcggcca catgagtgtc gcgcagttgg taggtctggc gcgtctgcgc gaggccgtcc
      841 tgccagcgcc cggagtgcgc gtgttgagcg aaccggcgcc cgacgaaagc gacgagcttg
      901 ttccggcggc ggttctcgtc ccggtagtga cccatggcga tccgactgtt ctgctgacgc
      961 tgcgcaccgc gcacctgcgt gaccatgccg ggcaggtcag ctttccgggc ggtcggatcg
     1021 aggatgacga cgcttcggca cggcacgcgg cgttgcgcga ggcggaagag gaaatcgggc
     1081 tagccaatca caacgtcgaa gtgatcggct atctgcccga gtatcgcacc gttaccgggt
     1141 tccggattac gccggtcgtt gggctggttc cgccaggcgt ggacttgaag tgcgatccct
     1201 tcgaagtatc ggcggtgttc gaggtgcctt tggccttcgt gctcgacccg cagaatcacc
     1261 gcaatcacca gatccactat cgcgggcggc tgcgtgaata cacggcggtg acttacggcg
     1321 actacctgat ctggggcgcg actgccggca tgctgcgctc gctgttcgaa ctgctcaggg
     1381 agccagtgcc gcgctgacgg cagcgacgaa ctcgtcggcc tttcggtgcc cggccatggc
     1441 gcggacgact tcctgatact tggccagcag cgcgcggaac tcctcggcat tggcggcacg
     1501 ctcggcagcc agagtgaagc ggtcggcgtc ggggccgagg gccgcgacca gacgctgaac
     1561 catcacgcgt ttggcgttgc gcagatcaac ggcggcttcc ggtgggtctg gtggaagtcc
     1621 ctctgactgc ccgagcagat gcaactgcac cagtccgtcg cggaacgcgt cggccttgcg
     1681 cctatcgctg gcaccgcgaa tgacatcgag gtattccagg gcaatcgcct tgaggctgcc
     1741 ggcatcgctc gccgcctcga tatgcacggt gaaatggtcg gcttcgggtc cgagggcgtc
     1801 gagcatggcc tgcgcgacgt agcgttttgc ggcaacgagt gcaaggtcgg gggagggcgc
     1861 ggatgcggcc gtatcgagcg gtgccgatgc tgcattcgac agctcgccgg catcgtcgat
     1921 tccaccgacg ggttcaataa atccgtcctt gagcagggac tcgatcagtt gctgtgcttc
     1981 ttccgggtgc ggcgcgcgcg caaacaattc tccggcgggc aggtggccgt cgatcatgat
     2041 caacagcgag cgcattcgtg cggggagccg gtaggtccgc cgcgtcattt cctcgaatcc
     2101 tttttgggtc ttcgcgtaga tcgtcccgct cggcattgtt ctcggttcct cgtcccccgg
     2161 gaagtataga ctcagccgca gtcggcggtg gcaagccggt tccggacgca ctggcccatg
     2221 tgataatctt gcggctgatg gtgtcggcgg accgcgacga cgccaccgga gtcggcaatt
     2281 tccccctcgg gattccttca aagcggcgtc tggccgcagc ccctgattac cgaaatgacc
     2341 tttctcgccc tacttgccgc actggtgatc gaacagttcc ggccgcttga tccggagcga
     2401 ttcatccgta cgcccgccga aatggccggc ggctggcttg agcagcaggc aaacgccgga
     2461 acctaccgtc atggcgtcat tgcgtgggtg ctggcggtgg gcgtggtgct gggcatggtg
     2521 gcgattgtct acggcattct ctgtgcaatg cacccggtgc tggcttggct gctcaatgtg
     2581 gtgatccttt actgggccat gggcttcaga caggtcagcc actatttcac gggtatccag
     2641 aaggcactgg ccgaggggcg cctcgacgac gcgcgattgc agctggcgaa ctggcgcaat
     2701 gaacgcaccg aacgcatgag ttcgcaggat gttacggggc tggccatcga gagcgcgctg
     2761 gttgcctcgc accggcaggt gttcgccgtg atgctctgtt tcgtgctgtt gccgggaccg
     2821 ttaggcgcgg tgctctatcg actcgcggag caactcgcgc gggtctggcg tccatctggc
     2881 gagagcgcgt tcggtaattt tgcgcaggag gcttttcgga ttatcgactg gctaccgcaa
     2941 cgggtgaccg ctttcggttt cgccgttgtc ggcgattttg aagatgcggt tcattgctgg
     3001 cgaacgcagg ctgagacctg gggcgacgcg gccggtggtg tcgtgctggc agcgggtgcc
     3061 ggtgcgctcg gcgtacagct gggcgccccg attaacgacg gaattgaagt cgtcgagcgg
     3121 ccggtgctcg gcctcggcga cgaccccggt atcgaatcgc tgcaggcgac cgtgggcttg
     3181 gtctggcggg ctctggtatt ctggatggtc attttgctgg cactcagcgt tgccggcgca
     3241 gcagggtagt ttgcatgagc aatccaacgg agaggaaaga taaatgagca atcgcgaagt
     3301 tgt
//
LOCUS       DATDAA010000089         5081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_41480,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000089 DATDAA010000000
VERSION     DATDAA010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41480"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="VIS73_02920"
     CDS             complement(<1..1428)
                     /locus_tag="VIS73_02920"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765267.1"
                     /note="catalyzes the conversion of citrate to isocitrate
                     and the conversion of 2-methylaconitate to
                     2-methylisocitrate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase B"
                     /protein_id="HEY5762137.1"
                     /translation="MLEAYRAHVAERAALGIPPLPLSKQQTSELVGLLKNPPKGEEAA
                     LVELLTYRVPAGVDDAAKVKAEFLAKLSSGADKCALISRAKATELLGTMLGGYNVKPM
                     IDLLGDAEVGGIAAEGLKKTLLVFDFFHDVAELAKKGNANAKSVMQSWADAEWFTSRP
                     EVPASQKLTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRPGIEADKAGERG
                     PVKQLEALAKKGNLIAYVGDVVGTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCIGS
                     KIAPIFFNTMEDAGALPIELDVNQMDMGDEIELKIDATTAKVTALKGGKVIAESQLKT
                     PVILDEVRAGGRIPLIIGRGLTTKAREALGLPVSTLFRLPQNPADPGKGYSLAQKMVG
                     RACGLPEGKGILPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHT
                     AAYPKLVDVRMHRELPSFISTRGGVA"
     gene            1775..2527
                     /locus_tag="VIS73_02925"
     CDS             1775..2527
                     /locus_tag="VIS73_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEY5762138.1"
                     /translation="MTASITLSEQSGVRYLHFGSEWIQGAMRIRRPHALELAYTREMM
                     LPLLLHEGPWPRRILMIGLGAGSLAKFVVRALPETHLQVVEINPEVPPVARSHFRLPE
                     EGARFRITLADGVEYVAQTRHRFDWILVDGYDRNARSGPLDQAPFYAACRARLTDHGL
                     LTTNLFQRSRGHQASISRLAAAFDKRAVALASADAGNVIGIAAAGRSLEVPLADMRAR
                     AEVVRRNTGLDLRPAISRLQLSAQLESGVLCL"
     gene            complement(2535..3218)
                     /locus_tag="VIS73_02930"
     CDS             complement(2535..3218)
                     /locus_tag="VIS73_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY5762139.1"
                     /translation="MGAWHRMLWTEGQRWLRRGLRLGCLACLPLGLAACSPSPPFSPL
                     PTNARVLVLGDSLVSGYGLSVQRAWPALLSQTTGWQIENAGVSGNTSEQGLARLPDYL
                     DVESPPAAVIVVLGGNDMLRKIPEAQTRANLATAIEKIRRVAAVPVLIAVPRPSLAGA
                     VLQSLDDADLYAELAAESNVPLIESVFSDVLSESALKLDRLHPNDAGQQELARRIADA
                     MRKLGLFAP"
     gene            3236..4471
                     /locus_tag="VIS73_02935"
     CDS             3236..4471
                     /locus_tag="VIS73_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5762140.1"
                     /translation="MNANSMAPSGSILSDYAGGSLVNLMQSVQSLLQAAPAIETYAPL
                     DPRHGLGELAGTAPSHIMLVVIDGVGCRQLDEHAPSGWLEANRRSTLTSVFPSTTASA
                     IPCFLSGLAPRCHGLLGWQIRDEVSGEIVNTFPLTQRSPGGATPLSPPAVARVLSYPP
                     LVHRLPGAHCTIAPAEIAGSDFNQAHSGGGPMIGYRDLAGFEEALQTACDYHRVAGRH
                     FADTPGFTYAYCPWPDTLMHRLGVSDPRVGEAIRGIDGCLSRLHARERGNGLAMIVTA
                     DHGFIDAPPERLIEIDADGLHADWLGFLDAPLWGERRVAFASVAPEQHDGFVRFVADR
                     YAHALDLLSADDFLAGPWLGSGQAHPALRQRLGDFVLLLKDDWTIKDWLPGERRYQHL
                     GVHSGASSDEMYVPLIVAS"
     gene            4903..>5081
                     /locus_tag="VIS73_02940"
     CDS             4903..>5081
                     /locus_tag="VIS73_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762141.1"
                     /translation="MMRQYLGLKSQHPDTLLFYRMGDFYELFYEDAEKAARLLDITMT
                     TRGQSAGQPIPMAGVP"
BASE COUNT          865 a         1751 c         1572 g          893 t
ORIGIN      
        1 tgcaacgccg ccgcgcgtcg agatgaacga cggcaactca cggtgcatgc gcacgtcgac
       61 cagcttcgga taggcagcgg tgtggcagaa cgactgcatc accatgtcgg ccgagaagcc
      121 gaggcaggcg aggtctttca attcgtcgcg ggtcatcggc ccggtggtgt cctgtgatcc
      181 gacggtggtc atcttcggct cgcagtaggt cccgggcagg attcccttgc cctcgggcag
      241 gccgcaggcg cggccgacca tcttctgcgc cagcgaatag cccttgcccg gatccgccgg
      301 attctgcggc aggcggaaca gcgtcgaaac cggcaatccc agtgcttcgc gcgccttggt
      361 cgtcaggcca cggccgatga tcagcggaat gcggccgccg gcacgcacct cgtcgaggat
      421 gaccggcgtc ttcagctgcg actcggcgat gaccttgccg cccttcagcg cggtgacctt
      481 ggccgtggtc gcgtcgatct tcagttcgat ctcgtcgccc atgtccatct ggttgacatc
      541 gagctcgatc ggcaatgcgc cggcgtcttc catcgtgttg aagaagatcg gggcaatctt
      601 cgagccgatg cagacgccgc cgaagcgctt gttcggaacg aagggaatat cctcgccagt
      661 gaaccagagc accgagttgg tcgccgactt gcgcgaggag ccggtaccga cgacgtcgcc
      721 gacgtaggcg atcaggttgc ctttcttggc cagcgcttcg agttgcttga ccggcccgcg
      781 ctcgcccgcc ttgtccgcct caatgccggg gcgcgggttc ttgagcatgg ccagcgcgtg
      841 cagcgggatg tccgggcgcg accaagcatc gggcgccggc gacaggtcgt cggtgttggt
      901 ctcgccggta accttgaata cggtcagctt ctgcgatgcc ggaacctcgg ggcgcgaggt
      961 gaaccactcg gcgtcggccc acgactgcat gaccgacttg gcgttggcgt tgcccttctt
     1021 ggccagctcg gcgacgtcgt ggaaaaaatc gaagaccagc agcgttttct tcaggccctc
     1081 ggcggcaatt ccgccaactt cggcgtcgcc cagcaggtcg atcatcggct tgacgttgta
     1141 gccgccgagc atcgtgccca acagctcggt cgccttggcg cgcgatatca gcgcgcactt
     1201 gtcggcaccg ctcgaaagct tcgccaggaa ctcggccttc accttggccg catcgtcgac
     1261 cccggccggc acgcggtagg tgagcagttc gacgagggcg gcttcctccc ccttcggcgg
     1321 gttcttcagc aacccgacga gttccgaggt ctgctgcttc gacagcggca gcggcgggat
     1381 tccaagggca gcacgttcgg caacatgagc acggtaggct tcgagcatgg cgctaacttc
     1441 tcctgaaaaa atgagtggga tcgctaacgg agtatccgag gcggcgcatg gcgagcacgc
     1501 gactttttat attatatgtc ttttataaga cttgataaac agcgggcggc ttgatctgcc
     1561 gaagaggctg tcagactgaa cacggcgaag gacgttcccc gccgcctgca atggccaaac
     1621 gaaacggccg ccgggtctcc ccgacggccg atcgctttgt tctccctgat tgctgcactg
     1681 ccatcacgta ctggctgtta tcctagccac gcaggccggc cgcgtattga tacaggtcat
     1741 aagaacccgc attagccagc cttaccaccc gccaatgacc gcctcaatca ccctcagcga
     1801 acagtccggc gtccgctacc tccattttgg gtcagagtgg attcagggcg ccatgcgtat
     1861 ccgcaggccg cacgcgctcg aactcgccta tacgcgcgag atgatgctgc cgctgctgct
     1921 gcacgagggg ccgtggccgc gccgcatcct gatgatcggg ctaggagccg ggtctctggc
     1981 caagttcgtc gtccgcgccc tgcccgagac gcacctgcag gtcgttgaga ttaaccccga
     2041 ggtgccacca gtggcccgca gccatttccg gctgcccgag gaaggcgcgc gctttcgcat
     2101 cacgctcgcc gacggcgtcg agtacgtggc gcaaacccgc caccgcttcg actggatcct
     2161 cgtcgatgga tacgatcgca acgcccgctc gggtccgctc gaccaggcgc ccttctatgc
     2221 ggcctgccga gcgcgactga ccgaccacgg gctgctgacg accaacctct tccagcgctc
     2281 gcgcgggcat caggccagca tttcccgcct ggcggccgcg ttcgacaagc gggcagtcgc
     2341 gctcgcgtcg gccgacgccg ggaacgtgat cggcatcgcc gccgccggcc gctcactgga
     2401 agtcccgctc gccgacatgc gcgcgcgtgc cgaagtggtc cgccggaata ccgggctcga
     2461 cctgcgcccg gcaatttccc ggctgcagct ttccgctcag ctcgaatccg gcgttctctg
     2521 cctgtagcga gccctcaagg cgcgaacagg cccagcttgc gcatggcgtc ggcaatccgg
     2581 cgtgccagtt cctgctgtcc cgcgtcgttc gggtgcagac gatcgagctt aagcgccgac
     2641 tcggaaagga cgtccgaaaa caccgactcg attagcggca cattgctctc cgcagcaagc
     2701 tcggcataga gatccgcgtc atcgaggctc tgcaggacgg cgccggccag cgagggccgc
     2761 ggcacggcaa tcaggaccgg caccgccgcc acgcggcgaa tcttctcgat cgctgtggcg
     2821 aggttggcac gggtctgtgc ctcgggaatt ttgcgcagca tgtcgttgcc gccgaggacg
     2881 acgatcaccg ccgccggcgg cgactcaacg tcgagataat ccggcagtcg cgccagcccc
     2941 tgctcgctgg tgttgccgct gacaccggcg ttctcgatct gccacccggt cgtctgtgac
     3001 agcagggctg gccaggcccg ttggaccgag agcccgtatc ccgagaccag gctgtcaccg
     3061 agcacgagga cgcgggcgtt ggtgggcaag ggggaaaacg gcggcgaggg gctgcaggcg
     3121 gcgagcccga gcggcaggca ggccaagcag ccgaggcgca agccacgacg cagccatcgc
     3181 tgcccttcag tccaaagcat ccgatgccag gcacccatgt cctacactcc tgctgatgaa
     3241 cgcgaactcg atggcgccaa gcggcagcat cctctctgat tatgccggcg gcagcctcgt
     3301 caatctcatg caatcggtgc aatcgctgct gcaagcggcg ccggcgatcg agacctatgc
     3361 gccgcttgac ccccgccacg gtcttggtga gctagcgggc acggcgccga gccatatcat
     3421 gctcgtggtc atcgacggcg tcggctgccg ccagctcgac gagcacgccc cgtccggctg
     3481 gctcgaggcg aaccgccgca gcaccctaac ctcggtcttt ccgtcgacga ccgccagcgc
     3541 catcccctgc tttctttcgg gactcgcgcc gcgctgccac ggattgctcg gctggcagat
     3601 ccgcgacgaa gtctcgggcg agatcgtcaa cacctttccg ctgacccagc gcagtcccgg
     3661 cggcgcgacg ccgctctcgc cgccggcggt cgcccgcgta ctctcctacc cgccgctcgt
     3721 ccatcgcctg cccggagcgc actgcacgat cgccccggcc gaaatcgctg gctctgactt
     3781 caaccaggcg cactcgggtg gcggcccgat gatcggctat cgcgacctcg ccgggttcga
     3841 ggaggccttg cagacggcct gcgactatca tcgcgtggcc ggccgccatt tcgccgacac
     3901 acccggtttc acctacgcct attgcccctg gcccgatacg ctgatgcacc gcctgggcgt
     3961 ctccgatccg cgcgtcggcg aggcaatccg cggcatcgac ggctgcctgt cgcgacttca
     4021 tgcgcgcgag cgcggcaacg ggcttgcgat gattgtcacc gctgaccacg gctttattga
     4081 tgcgcccccc gaacgcctga tcgagatcga tgccgatggc ctccacgccg actggctcgg
     4141 ttttctcgac gcgccattat ggggcgagcg ccgcgtcgcc ttcgccagtg tcgcgcccga
     4201 gcagcacgat ggctttgtcc gctttgtcgc cgaccgctac gcgcacgcgc tcgatctgct
     4261 gagcgccgat gactttctcg ccggcccctg gctcggcagc ggccaggcgc atccggcgct
     4321 gcgccagcgc ctcggagatt tcgtcctgct gcttaaggac gactggacga tcaaggactg
     4381 gctgccgggc gagcgccgct accagcacct cggcgtgcac agcggcgcct catccgacga
     4441 gatgtacgta ccgctaatcg tcgccagctg acacgcaacc cccaccggcc cgcctgcaga
     4501 tgctgccgtg caggagcgcg caagccgctg tttttcagag atttttgcga ccaggtgcag
     4561 cctgtggaaa aacctgtgca caggattcag acactgcacg cgatacaacg gatgtcaaga
     4621 ccccggtgag tggcgaaacc ccccatccag atatttttcc aatgtttttc agacgcttgc
     4681 acgatccatt ggggccggca gggagatcgt gttgtgcaga ggcaacatac gccgccactg
     4741 tgcataagtg tcggggaaaa cgaggaaatt cgaaacggca acgaggtccg ctacaatcgg
     4801 ccgcccaacc aggaacacgg cccgtcccgg cgccgtgcgc gcccttcgaa tgcacctgac
     4861 gtgacggcac ccagtcaagc caccctcgcc gaccatacgc cgatgatgcg ccagtatctc
     4921 ggcctgaaat cccagcatcc ggatacgctg ctcttctacc ggatgggcga tttctacgaa
     4981 ctcttctacg aggacgccga gaaggccgcc cgcctgctcg acatcacgat gacgacccgc
     5041 ggacaatcgg ccggccagcc gattccgatg gccggcgtcc c
//
LOCUS       DATDAA010000090        12088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_216535,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000090 DATDAA010000000
VERSION     DATDAA010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12088
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216535"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="VIS73_02945"
     CDS             <1..135
                     /locus_tag="VIS73_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5762142.1"
                     /translation="AEVDEAVKALSSLMEPIIMVVLGGLIGGLVVAMYLPIFKLGSVV
                     "
     gene            164..1018
                     /locus_tag="VIS73_02950"
     CDS             164..1018
                     /locus_tag="VIS73_02950"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEY5762143.1"
                     /translation="MTEISVAPWAAIAGLLGLIVGSFLNVVIHRLPRMLEAEWRTQCA
                     ELAGSPAGDAGERYNLLFPPSHCPSCRAPIRPWQNIPLISFALLRGRCRHCRERISWR
                     YPLVEAAAGVLAALVIWKFGPTLAGASALVFAWTLLALSVIDLDTQLLPDALTLPLIW
                     LGLLVNLGNTLTPLPSAVVGAVVGYLVLWSVYWAFRLLTGKEGMGYGDFKLLAAIGAW
                     LGWSMLPLVILASSFVGAVVGITMMVLRRHTRGTPMPFGPYLAAAGLIGLLWGTELTG
                     RYLALFPG"
     gene            1115..1378
                     /locus_tag="VIS73_02955"
     CDS             1115..1378
                     /locus_tag="VIS73_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY5762144.1"
                     /translation="MPIYEYRCNSCGFQKEFLQKISDDPIKTCPECGKESFDKLLSAA
                     GFQLKGSGWYATDFKGGAKPPAAKADNPPPCQGGTGACPAADG"
     gene            1375..1998
                     /locus_tag="VIS73_02960"
     CDS             1375..1998
                     /locus_tag="VIS73_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEY5762145.1"
                     /translation="MKRYLITGLIIWAPLAITIWVLQFFVGVLDQSLNLLPEHYHPRQ
                     LFGFYLPGIGAVLTIAVVMVTGWLVTHILGERLISWWNGFLNRIPVLGAIYKAVKQVS
                     DTLLKPGGHAFRKVLLVRYPHPQAWSLAFQTSVPGEVSRRLDDDYVAVFIPTTPSPVN
                     GFYFYVRKAETIELDMSVDVALRTIVSMGVVTSPDNGRSPIIATPAA"
     gene            2028..3839
                     /gene="aspS"
                     /locus_tag="VIS73_02965"
     CDS             2028..3839
                     /gene="aspS"
                     /locus_tag="VIS73_02965"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY5762146.1"
                     /translation="MRTHYCGQLSAKLVDQTVSLCGWAHRRRDHGGVIFIDLRDRDGL
                     AQVVCDPDRPEMFRIAESVRNEFCLRITGRVRRRPAGTENKNLASGEIEILCQEIEVL
                     NPSVTPPFQLDEDNLSENVRLTHRVIDLRRPQMQNNLLLRYKVARAFRRFLDDSGFID
                     IETPMLTKSTPEGARDYLVPSRVHPGQFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQVDIETSFMSEIEITALMEQLIRTVFKDAIDVDLPAFPRMSYAEA
                     MARFGSDKPDLRVTLELTEMTDAFKDVAFKVFANVANSDGGRIAALRIPGGGSGAKAL
                     TRGEIDAYTQFVGIYGAKGLAYIKVNDVAQPNESGLQSPIVKNLSEASLRTVLERTGA
                     QSGDLIFFGADKAKIVNDALGALRVKIGHEKGFLSGEKWAPLWVVDFPMFEYDDEAKR
                     WNACHHPFTSPKDGHLDLLTSDPGKCLAKAYDLALNGWELGGGSVRIHRADVQEKVFA
                     ALAIGPEEQRAKFGFLLDALKYGAPPHGGLAFGLDRIVTMMTGAESIRDVIAFPKTQR
                     AQCLLTDAPSPVDEKQLRELHVRLRQKIETQVEVSAT"
     gene            3861..4322
                     /gene="nudB"
                     /locus_tag="VIS73_02970"
     CDS             3861..4322
                     /gene="nudB"
                     /locus_tag="VIS73_02970"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEY5762147.1"
                     /translation="MSGNKRPLSVLVLVHTGAGQILLLERQQHPDFWQSVTGSLEPGE
                     GHAAAAVRELEEETGLRVDAALLLDTRIENRFEILPLWRDRYPEGVTHNSERVFECEL
                     TAACAIRIAPDEHRASIWLPWRQAAAKVFSWTNRDAIQRLAIRRGWAAEPS"
     gene            4582..5472
                     /gene="argB"
                     /locus_tag="VIS73_02975"
     CDS             4582..5472
                     /gene="argB"
                     /locus_tag="VIS73_02975"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY5762148.1"
                     /translation="MNATTARSPQDIAAILAEALPYIRRFHGKTLVVKYGGNAMTDEH
                     LKHSFARDVVLLKLVGMNPVVVHGGGPQIETLLGRVGKKGVFIQGMRVTDAETMELVE
                     MVLGGQVNKEIVNLINQAGGKAVGLTGKDGNFIRARKLMMANKDIPGDLIDIGQVGEI
                     VQIDPSLIGHLDQGQFIPVIAPIGVGAEGETYNINADVVAGKIAEVLRAEKLVLLTNT
                     PGVLDAKGKLLTGITPAEIDDMVTDGTLSGGMLPKIGSALDAARSGVRSVHIIDGRVE
                     HALLLEILTDHGVGTMIRSR"
     gene            5487..6131
                     /locus_tag="VIS73_02980"
     CDS             5487..6131
                     /locus_tag="VIS73_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEY5762149.1"
                     /translation="MPGHSRLWVFDLDNTLHDASARIFPLINRAMREYIQRHLGLDEH
                     GATHLRQDYWRRYGATLEGLVRHHGVDPAHFLFETHRIADLHAHVEVDPRLGATLAQL
                     KGRKIVFSNGPAHYAQAVLGAMNLRHRFDRIYSIESVGFRAKPDPSSFRFVLREEKTR
                     AADAIMVEDSIDNVRSAKRVGMKTVWVSSSGKPCRDADRIVASVHELPRIAASV"
     gene            6166..6756
                     /gene="slmA"
                     /locus_tag="VIS73_02985"
     CDS             6166..6756
                     /gene="slmA"
                     /locus_tag="VIS73_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEY5762150.1"
                     /translation="MAAKPGERRLQILQVLAEMLETPKGEKITTAGLAKRLDCSEAAL
                     YRHFASKAQMFEGLIDFIETSLFGVINQIVQDDSNALRQIEHIMALLLRFAAKNRGMT
                     RVLIGEALVNEDERLQARINQLTDKIEASLRQCVKVACSEGQLPPQGDSAAIANLLLC
                     YAIGRWQLYAKSGFTREPAAQWAGQWPLLLSAAAVR"
     gene            complement(6766..7779)
                     /gene="hemB"
                     /locus_tag="VIS73_02990"
     CDS             complement(6766..7779)
                     /gene="hemB"
                     /locus_tag="VIS73_02990"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY5762151.1"
                     /translation="MANEGMFPGTRMRRMRRDAFSRRLMCEHTLTASDLIYPVFVLDG
                     RGQRETVASMPGVERQSLDLLLKTAELAQELGVPALALFPVVGAEGKSWDAEEAYNPD
                     GLVPRVVQALKARFPDLGVITDVALDPYTIHGQDGLIARDDPRAYVLNDETLEVLAKQ
                     ALTHAQAGADVVAPSDMMDGRVARIRAALDADRKIHTRILAYSAKYASAYYGPFRDAV
                     GSSGNLGGGNKYTYQMDPANSDEALREVALDLAEGADMVMVKPGMPYLDIVRRVKDRF
                     GVPTYAYQVSGEYAMIKAAAQNGWLDEKACMLEALLGFKRAGADGVLSYFALDAAEAL
                     RNR"
     gene            complement(7905..8552)
                     /gene="yihA"
                     /locus_tag="VIS73_02995"
     CDS             complement(7905..8552)
                     /gene="yihA"
                     /locus_tag="VIS73_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY5762152.1"
                     /translation="MPFLRHLAFLTTVANLRQLPDDSLAEVAFAGRSNAGKSSAINTL
                     ANHTRLAHTSKTPGRTQHLNFFTVQPGRFLVDLPGYGFAKAPEEIRGQWDSLIGPYLA
                     KRDALKGLVIIMDSRRPMTELDCRLIDWFAPTGRPIHILLSKADKLTRQEQAKAIAET
                     RGFLKNWTVPVSVQLFSSLKKTGVEEADQLIGGWLGLGDLEAAKKEPPGKGMPGG"
     gene            8710..9330
                     /locus_tag="VIS73_03000"
     CDS             8710..9330
                     /locus_tag="VIS73_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5762153.1"
                     /translation="MFHRPLLALTLAVFAVATQAAGDPAKGKPIGSTLCAGCHGLDGQ
                     SLIPGYPHLAGQFPEYMAKQLRNFKAGGDQPALRKNDIMTGMAMTLSDEDVVNIAAYF
                     AEMTPKASAARSGADLALGKKIWQGGLLDKGVPACAGCHGAAGHGLPVQYPRIANQSP
                     DYTEAQLKAFRTGDRANDPEKMMRMIAEKLNDAEMRAVADYAATLQ"
     gene            9450..9884
                     /locus_tag="VIS73_03005"
     CDS             9450..9884
                     /locus_tag="VIS73_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5762154.1"
                     /translation="MSTTIKQILDSKKKPLSTVGPEDTVQKAVELMQSQGIGAVLVID
                     GKKLVGIFTERDCVQKVTLSAHDPRQTKVRDVMTERVRFITPDLSVSECLALMTERYF
                     RHLPVLDEQENILGIVSIGDLVKAKISEQTFIIEQLERYITS"
     gene            10018..10527
                     /locus_tag="VIS73_03010"
     CDS             10018..10527
                     /locus_tag="VIS73_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY5762155.1"
                     /translation="MRQITEHLPRLRRYARALTGDRYAADDLVQDTIERALSRAALWQ
                     AGSKLDAWLLTIMHNLFVNGVRSAASRLTVPSDDDEFERRPGPSADPSARLAIRDLDA
                     ALAQLPVDQREVLLLVALEDTPYEEAARILGIPIGTVMSRLSRARDRLRKLLAGEPVS
                     PMHLKVVRQ"
     gene            10524..11315
                     /locus_tag="VIS73_03015"
     CDS             10524..11315
                     /locus_tag="VIS73_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY5762156.1"
                     /translation="MTTPLERDLHAYADGRLDEAEAARVAAWLAEQPEAAARVLDWQR
                     QNEQLHFAFDPVLDEAVPDRWLTRIADARRQRARPLAIAAAWIALGGVLGYLVGTLPT
                     GASLAPAPTSSIGASPLPRQAAIAHVIYSPEVRHPVEVGADQEAHLVQWLSKRLGQPI
                     RIPALGERGFALVGGRLLPGDRGPVAQFMYENPQGTRLTLYVSPPAENDRESAFRFAD
                     EAGVGVFYWVDPRFAYALAGDLDRDTLLAIAETAYHQLDRAGAGL"
     gene            11399..11998
                     /locus_tag="VIS73_03020"
     CDS             11399..11998
                     /locus_tag="VIS73_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEY5762157.1"
                     /translation="MLAGASPFPGIGERDADLLRDSGLSALLLTVLATAVPTLRQRLG
                     LPWLLRLRQPIGALAFAYALAHGLLAMRAAGGSRVSELLRAAVEEPAQLAGLASFALA
                     LPLLATSNRLALRWLSAPVWQDVQRSLGIVAALATAHAIGLVTATPRLIIVLLAAAAL
                     VWIAFAAARSLRDRLRQPATPPAAPGGQVLRFYRRRPPR"
BASE COUNT         2157 a         4061 c         3697 g         2173 t
ORIGIN      
        1 gccgaggtcg acgaggcggt caaggcgctt tccagcctga tggagccaat catcatggtc
       61 gtcctcggcg gcctgatcgg cggtctggtg gtcgccatgt acctgccgat attcaagctc
      121 ggctcggtag tttgatcgcg caaccgcgac cccgacgatc ctgatgacag aaatctcggt
      181 ggccccatgg gccgcgattg cgggccttct cggcctcatc gtcggcagct tcctcaacgt
      241 cgtgatccac cgcctgccgc ggatgctcga ggccgaatgg cgaacgcagt gcgccgagct
      301 ggccggttcc cccgccggcg acgccggcga gcgctacaac ctgctctttc caccttcgca
      361 ttgcccgagc tgtcgcgcgc cgataaggcc ctggcaaaac atccccctga tcagtttcgc
      421 gctgctgcgc ggccgctgtc ggcattgccg cgaacggatc agctggcgtt atccgcttgt
      481 agaagcagcg gcgggtgtgc tggcggcgct ggtgatctgg aaattcggcc cgaccttggc
      541 cggtgctagc gcccttgtct ttgcctggac cctactggcg ctatcggtca tcgacctcga
      601 tacgcaactg ctgcccgatg cactgaccct gccactgatc tggcttggcc tgctggtcaa
      661 cctcggcaac acgctgacgc cgctgccatc ggcggtagtc ggcgcggtcg tcggctacct
      721 cgtgctctgg tcggtctact gggcgtttcg cctgctgacg ggcaaggaag gcatgggtta
      781 cggcgatttc aagctgctgg ccgcaatcgg tgcctggctt ggctggtcca tgctgccact
      841 ggtcatcctt gcctcgtcct tcgtcggcgc cgtcgttgga atcacgatga tggtcctgcg
      901 ccgacatacc cgcggcacgc cgatgccctt cggtccttat ctcgcggctg ccggcttgat
      961 cggcctgctc tggggaaccg aactgaccgg ccgttacctc gcgctgtttc ccggctgaaa
     1021 agccagggct tgcgtttccg gtgctcgccc ccagcttgaa ccgcggggaa atcgagcccc
     1081 gctccgctat aatcgcgcgc tcccggagac tgccatgccc atctacgaat accgatgcaa
     1141 cagttgcgga ttccagaagg aattcctgca gaaaatcagt gatgacccga tcaagacctg
     1201 cccggaatgc ggcaaggaaa gcttcgacaa gctgctgtca gctgccggct tccagctcaa
     1261 ggggtcgggc tggtacgcta cggatttcaa gggcggcgcc aagcctcctg cagcaaaggc
     1321 cgacaatccg ccgccttgcc agggcgggac tggcgcctgc ccggctgccg acggatgaaa
     1381 cggtacctga tcaccggcct gatcatctgg gcgccgctag cgatcacgat ctgggtactg
     1441 cagttcttcg ttggcgtcct cgaccagtcg ctgaacctgc tacccgagca ctaccatccg
     1501 cggcagctgt tcgggttcta cctgccggga atcggcgcgg tcctcaccat cgccgtcgtc
     1561 atggttaccg gctggctggt cacgcacatc ctcggcgaac gattgatttc ctggtggaat
     1621 ggctttctca accgcattcc ggttctcggg gcgatttaca aggccgtcaa gcaggtttcc
     1681 gataccctac tgaagccggg cgggcacgcc ttccgcaagg ttctgctggt ccgctacccg
     1741 catccgcagg cctggtcact ggcgttccag accagcgttc ccggcgaagt atcgcggcgc
     1801 ctcgatgacg attacgtcgc ggtcttcatc ccgaccacac cgagcccggt caacggcttc
     1861 tatttctacg tccgcaaggc cgagaccatc gagctagaca tgtcggtcga tgtcgcgctg
     1921 cgcacgatcg tatcgatggg cgttgtcacg tcgcccgaca atggccgatc acccataatt
     1981 gccaccccgg cggcctgacg cctgcaaacc aaaaagaatc cggaaccatg cgcactcatt
     2041 attgcggcca gttgtcggcc aaacttgtcg atcaaaccgt ttctttgtgc ggctgggctc
     2101 atcgtcggcg cgatcacggc ggcgtcattt tcatcgacct gcgcgatcgc gacggtctgg
     2161 cgcaggtcgt ctgcgatccg gaccggcccg agatgttccg tatcgccgag agcgttcgca
     2221 atgagttctg cctgcgaatc accggccggg ttcgtcgtcg accggccgga accgaaaaca
     2281 agaacctggc ctccggtgaa atcgaaatcc tttgccagga aatcgaggtc ttgaacccat
     2341 cggtcacgcc gcctttccag ctcgatgaag acaacctctc ggagaacgtg cgcctgaccc
     2401 atcgcgtcat cgacctgcgc cgtccgcaga tgcagaacaa cctactgctg cgctacaagg
     2461 tcgcgcgcgc cttccgccgc ttcctcgacg acagtggctt tatcgacatc gaaacgccga
     2521 tgctgaccaa gagcacgcca gaaggtgcgc gcgattacct agtcccctcg cgcgtccatc
     2581 ccggtcagtt tttcgccctg ccgcaatcgc cgcagttgtt caagcagctg ctgatggttg
     2641 ccggtttcga ccgctactac cagatcacca agtgcttccg cgatgaggat ctgcgcgcgg
     2701 accgccagcc cgagttcacc caggtcgata tcgagacctc gttcatgagc gagatcgaga
     2761 tcaccgcgct gatggagcag ctgattcgca cggtgttcaa ggacgcgatc gacgtcgatc
     2821 tgccggcttt cccgcgcatg tcctacgccg aggccatggc ccgcttcggc tccgacaagc
     2881 ccgacctgcg cgtcaccctc gaactaaccg agatgaccga cgccttcaag gatgtcgcct
     2941 ttaaggtctt cgccaatgtc gccaacagtg atggcggccg catcgcggcg ctgcgcatcc
     3001 cgggtggcgg atcgggggcg aaagcgctga cgcgtggcga aattgacgcc tatacgcagt
     3061 tcgtcggcat ctatggcgcc aagggtcttg cctacatcaa ggtcaacgat gtcgcgcagc
     3121 ccaatgaaag cggcctgcaa tcaccgatcg tcaagaacct gtcagaggca tcgctgcgca
     3181 cagtgctcga acgcaccggc gcgcaatccg gcgacctgat cttctttggc gccgacaagg
     3241 cgaaaatcgt caacgacgcg ctcggcgcgc tgcgagtcaa gatcgggcac gaaaaaggat
     3301 ttctctcagg cgagaagtgg gcaccgctgt gggtcgtcga cttcccgatg tttgaatacg
     3361 acgacgaagc caaacgctgg aacgcctgcc atcacccgtt caccagtccc aaggacggcc
     3421 acctcgacct cctgaccagc gacccgggca agtgtctggc gaaggcatac gacctggcgc
     3481 tgaacggctg ggagctcggt ggcggttcgg ttcgtatcca tcgtgccgat gtccaagaga
     3541 aagtgtttgc cgcacttgcc atcggccccg aggaacaacg cgccaagttc ggcttcctgc
     3601 tcgacgcgct gaagtacggt gcgcccccgc acggcgggct cgctttcggc ctcgaccgca
     3661 tcgtcacgat gatgaccggc gccgaatcga tccgcgacgt gatcgccttt cccaagaccc
     3721 agcgcgccca gtgtctgctg accgacgcgc cgtcgccggt cgacgaaaag cagttgcgcg
     3781 aactgcacgt tcgtctgcgc cagaagattg aaacccaggt cgaggtcagc gcgacctaag
     3841 ggcgccgagc cccgaccgcg gtgagcggaa acaagcgtcc gctgtcagtt ctcgtcctgg
     3901 tgcacacggg ggcgggtcag atactgctgc tcgagcgcca gcagcatccg gatttttggc
     3961 aatcggtaac cggcagcctt gagcccggcg aaggccacgc cgccgccgcc gtccgcgagc
     4021 ttgaagaaga gaccgggcta cgagtcgatg cggcgttgct gctcgacacg cggatcgaga
     4081 accgattcga gatcctgccg ctctggcgcg atcgttatcc cgagggtgtg acgcacaaca
     4141 gcgagcgcgt tttcgagtgc gaactgacgg ctgcctgcgc gattcgaatc gctcccgacg
     4201 agcaccgcgc aagcatctgg ctgccctggc gacaggcggc ggcgaaggtc ttctcgtgga
     4261 ctaaccgcga cgcgattcag cgcctggcga tcaggcgcgg ctgggctgcg gaaccgagct
     4321 gagcctcttc tccagacaga gccggcagta acaggtgcca ctggcctcgc cgggcaaggg
     4381 cagcagtgcc ggcaactcgg cgcaccagca agtggcttcg ccacgggcgg cgccgcagga
     4441 caccggctct ccgcaaccgg ggcagacgct tccctggatt tcttcactca tcgatatcct
     4501 ctggtttccg ccaccggctg cgcgcctttc gtggccacgc cctgctcagt agaatggcgg
     4561 ctctcccgca acgagaccaa catgaacgcc accaccgctc gctcgccgca agacatcgcc
     4621 gccattctcg cagaagccct gccctacatc cgccgctttc acggcaagac gctggtcgtc
     4681 aagtacggcg gcaatgcgat gacggacgaa cacctgaagc acagcttcgc gcgtgacgtc
     4741 gtgctgctca agctggtcgg catgaatccg gtcgtcgtcc acggtggtgg cccgcagatc
     4801 gagaccctgc ttggaagagt cggcaagaag ggcgtgttca ttcagggcat gcgcgtaacc
     4861 gatgcggaga ccatggaact cgtcgagatg gtgctcggcg ggcaggtcaa caaggagatc
     4921 gtcaacctga tcaaccaggc cggcggcaag gcggtcgggc tgaccggcaa ggacggcaat
     4981 ttcatccgcg cccgcaaact gatgatggcg aacaaggaca tacctggcga cctcatcgac
     5041 atcggccagg ttggcgagat cgtgcagatc gacccctcgc tgatcggcca cctcgaccag
     5101 ggccagttca tcccggtcat cgcgccgatc ggtgtcggcg ccgaaggcga gacctacaac
     5161 atcaatgccg acgtggttgc cggcaagatc gccgaggtgc tgcgcgccga aaaacttgtc
     5221 ctgctgacca acacacccgg cgttctcgac gctaagggca agctgctgac cggcatcaca
     5281 cccgccgaga tcgacgacat ggttaccgat ggtactttat cgggcggcat gctgccgaag
     5341 attggctcgg cgctcgatgc cgcacgaagc ggagtcagga gcgtgcacat catcgatggt
     5401 cgggtcgaac acgcactgct gcttgagatc ctcaccgacc acggggtcgg cactatgatc
     5461 cgctcgcgct gaaccggact ctgggcatgc cgggccattc gcgcctctgg gtattcgacc
     5521 tcgacaacac attgcatgac gcctcggccc gcatcttccc gctaattaat cgggccatgc
     5581 gcgagtacat ccagcgacac ctcgggctcg acgagcacgg cgccacccac ctgcggcagg
     5641 attactggcg ccgctacggc gcgacgctag aaggactggt tcgtcatcac ggcgtcgacc
     5701 ctgcgcattt tcttttcgag actcaccgga ttgccgacct gcacgcacac gtagaggtcg
     5761 atccccgcct tggcgcgact ttggcccagc tgaagggacg aaaaattgtt ttttctaatg
     5821 ggcccgcaca ctatgcgcag gcggtgctcg gcgcaatgaa tctgcgccac cgttttgacc
     5881 gcatctactc gatcgaaagc gtcggctttc gcgcgaagcc cgacccatcc agctttcgct
     5941 tcgtgctgcg cgaggagaag acccgtgccg ccgacgcgat catggtcgag gacagcatcg
     6001 ataacgtgcg cagcgccaag cgggtcggga tgaagaccgt ttgggtgagt tcgagcggca
     6061 aaccctgccg tgacgccgac cggatcgtcg cctccgtcca cgaactgccg cggatcgccg
     6121 catcggtata atccgcgcca ataccaagaa ttaagggaga ccgcgatggc cgcaaagccg
     6181 ggtgagcgcc gactgcagat tctgcaggtc cttgcggaaa tgctcgaaac cccgaagggc
     6241 gagaagatca ccacggccgg cctggcaaag cggctcgact gttcggaggc cgcgctctat
     6301 cgtcacttcg ccagcaaagc gcagatgttc gaagggctga tcgatttcat cgaaacaagc
     6361 ctctttggcg tcattaacca gatcgtccag gacgactcaa atgcgctgcg gcagatcgag
     6421 cacatcatgg cgctgctgct ccgtttcgcg gccaagaacc gcgggatgac acgggtgctg
     6481 atcggcgagg cactggtcaa cgaggatgaa cgcctgcagg ccagaatcaa ccagctgaca
     6541 gacaagatcg aggcgagcct gcgccagtgc gtcaaggtcg cctgctcgga aggtcagctg
     6601 ccgccgcagg gcgactcggc ggcaattgcc aacctgctgc tctgctacgc gatcggccgt
     6661 tggcagctct acgccaagtc gggcttcacg cgcgaaccag cggcgcagtg ggccgggcag
     6721 tggccattgc tgctgtcggc cgccgcggtc cgctgagcga gcgaatcacc tgtttcgcag
     6781 tgcttcggcg gcgtcgagcg caaagtagct cagcacgccg tcggcaccgg cccgtttgaa
     6841 gccgagcagc gcctcgagca tgcaggcctt ttcatcgagc cagccgtttt gtgccgcggc
     6901 cttgatcatc gcgtactcgc cgctgacctg gtaagcgtag gtcggcacgc cgaatcgatc
     6961 cttcacgcgg cggacgatgt cgaggtaggg catccccggc ttgaccatga ccatgtcggc
     7021 gccctcggca agatcgagtg cgacttcgcg cagtgcctcg tcgctgttgg cgggatccat
     7081 ctggtaggtg tacttgttgc cgccgccgag gttgccggac gaaccgactg catcgcggaa
     7141 ggggccgtaa tacgcggagg cgtacttcgc cgaataggcg aggatgcgcg tatggatttt
     7201 ccggtcggcg tcgagcgccg cccgtatccg tgcgacacgg ccgtccatca tgtcggatgg
     7261 cgcgacgaca tcggcgcccg cctgggcgtg ggtaagcgcc tgcttcgcca gcacctcgag
     7321 ggtctcgtcg ttaagcacgt aggcgcgcgg gtcgtcgcgg gcgatcagcc cgtcctgtcc
     7381 gtggattgtg taggggtcga gcgccacgtc ggtgatcacg cccagatcag gaaaacgcgc
     7441 cttcagtgcc tgcacgactc ggggcaccag accatcgggg ttgtaagcct cctcggcatc
     7501 ccagctcttg ccttcggcgc cgaccaccgg aaaaagcgcg agcgccggca cacccagttc
     7561 ctgagccagt tcggcagtct tcagcaacag gtcgagcgac tgccgctcga cgcccggcat
     7621 cgacgccacc gtttcccgct gtccgcgacc gtcgagcaca aaaaccggat aaatcagatc
     7681 gctggcagta agtgtgtgct cgcacatcaa acgccgtgaa aacgcgtcgc ggcgcatgcg
     7741 acgcattcgc gtacctggaa acattccttc gttggccatc gtcaatctcg tcctcttcaa
     7801 tcgtcgggaa ctttccttga gttcccaaag tcacaatgcg cagatggttc acgccgtctc
     7861 ccgcttcacc tccctgagcg ggtgccttaa tcacgttaag gcagttaacc cccgggcatc
     7921 ccctttcccg ggggttcttt ttttgcggcc tcaagatcgc cgagaccgag ccagccaccg
     7981 atcagttggt cggcctcctc gactccggtc ttcttcaggc tcgagaacaa ttgaacgctg
     8041 accggcacgg tccagttctt gaggaaacca cgggtctcag caatcgcctt cgcctgctcc
     8101 tgccgcgtga gtttatcggc cttcgacagc aggatgtgga tcggcctgcc ggtcggtgca
     8161 aaccagtcaa tcagccggca atcgagttcg gtcattggcc gccgtgaatc catgatgatc
     8221 actagtccct tgagcgcgtc gcgctttgcc agataggggc cgatcagcga atcccattgt
     8281 ccgcgtatct cctctggcgc cttggcgaag ccataaccgg gaagatcgac cagaaatcgg
     8341 cccggctgca ccgtaaagaa gttgaggtgc tgggtgcgcc ctggggtctt gctcgtgtgc
     8401 gctaggcgcg tgtggttggc gagcgtgttg atcgccgatg acttgccggc gttggaacgg
     8461 ccggcaaacg caacctcggc cagggaatca tcgggcagct gacgcaggtt cgccaccgta
     8521 gtcaggaacg ccaagtgtcg caggaagggc atttcgggca ctcacccggc gcgacacgtg
     8581 ccgcgcgagt tgtgtaaaat cgcgagttta tggctttcgg tggccgcgac caagcgtggg
     8641 tacgcgcttc gacaagttcg cggccctcgt tttgatcatc ccgccttcac tgcccgagga
     8701 gttttcgtca tgttccaccg cccgcttctg gcgctgaccc tagctgtatt cgctgtcgcc
     8761 acccaggccg ccggagatcc ggccaaggga aaaccgatcg gctcgacgct gtgcgccggc
     8821 tgccacggcc ttgacggtca aagcctgatc ccgggctatc cgcacctggc cgggcagttt
     8881 cctgagtaca tggccaagca gctgcgcaac ttcaaggcgg gcggcgacca gccggcgttg
     8941 cgcaagaacg acatcatgac cggcatggcg atgaccctgt ccgatgagga cgtggtcaac
     9001 atcgcggcct attttgccga aatgactccg aaggcgtcgg cggcgcgaag cggcgccgac
     9061 ctcgcgctcg gcaagaaaat ctggcagggt ggcctactcg acaagggtgt tccagcctgc
     9121 gccggctgcc acggcgctgc cggacacggc ctgccggtgc agtatccgcg catcgccaac
     9181 cagtcgcccg attacactga ggcacagctc aaggccttcc gtaccggcga tcgcgccaac
     9241 gatcccgaaa agatgatgcg aatgatcgcg gaaaagctca acgacgccga aatgcgcgcc
     9301 gttgccgact acgcagcgac gctgcagtaa gtcctcagcg atggcgaccg acggcgggca
     9361 attcccgtcg tcgattgccg caaacagata gatacaggtc cgaaaccggg cgcgcccaac
     9421 aaagccaccc gctgccaccg gagacatcaa tgagcaccac gatcaagcaa atcctcgaca
     9481 gcaagaaaaa gccgctctcg actgtcggcc ccgaagacac cgtgcagaag gcggtcgagc
     9541 tgatgcagtc gcagggcata ggcgccgtac tcgtcatcga cggcaagaag ctcgttggca
     9601 tcttcaccga gcgcgactgc gtgcagaagg tcaccctcag tgcccacgac ccgcgccaga
     9661 ccaaggtgcg tgacgtaatg accgagcgag tccgcttcat cacacccgac ctgagcgtct
     9721 cggagtgcct ggcgctaatg accgagcgct atttccgcca ccttcccgta ctcgacgaac
     9781 aggagaacat tctcggcatc gtctcgatcg gggacctggt caaggcgaag atatccgagc
     9841 agactttcat catcgagcag cttgagcgtt acatcaccag ttgatccgta tccccggcaa
     9901 cggaccgacc gtcgccgaca ggaattcccg cgtgccgaag ggcatcgcgg gaattttttt
     9961 gcccgtcgcc tgttcaatgc tcatgccccg cacgaaccca accgccgatc cccttccatg
    10021 cgccagataa ccgagcacct accgcgactg cgtcgctacg cgcgggcgct gaccggcgac
    10081 cgctatgcgg ccgacgatct ggtgcaggac acgatcgaac gcgcgctgag ccgcgcggcg
    10141 ctttggcagg ctgggtcgaa actcgacgct tggctgctga cgatcatgca caacctgttc
    10201 gtcaatggcg tgcgctcggc agcctcgcgc ctgaccgtcc ccagcgacga cgatgagttc
    10261 gagcggcggc ccggcccgag cgccgacccc tcggcgcgac tggcgatccg tgacctcgac
    10321 gcggcgctgg cccagttgcc agtcgaccag cgcgaagtgc tgctgctcgt cgcgctcgag
    10381 gacacccctt acgaagaggc cgcgcgcatt ctcggcattc ccatcggcac cgtgatgtcg
    10441 cggctctcgc gcgcccgtga tcgcctgcgc aagctgctcg ccggcgagcc ggtctcgccg
    10501 atgcacttga aggtggtccg ccaatgacga caccgctcga acgtgatctc cacgcctacg
    10561 ccgacggccg cctcgacgag gcggaagcgg cgcgcgtcgc ggcctggctt gccgagcagc
    10621 cagaggccgc ggcgcgcgtg ctcgactggc agcggcagaa tgaacagttg cattttgcct
    10681 tcgacccggt gctagacgag gccgtacccg atcgctggct gacgcggatc gccgacgcgc
    10741 gtcgccagcg cgctcggccg ctggccatcg ccgccgcctg gattgccctc ggcggcgtcc
    10801 ttggttatct ggtcggcacg ctgcccacgg gcgcttcgct cgccccggcg ccgacctcat
    10861 ccattggcgc ctcgcccctg ccgcggcagg ccgcgattgc ccacgtcatc tattcgcccg
    10921 aggtccgcca cccggtcgag gtcggtgccg atcaggaagc ccacctcgtg cagtggctct
    10981 ccaagcggct cggccagcca atccgcatcc cggctctcgg cgagcgtggc tttgcgctgg
    11041 tcggcggccg gctgctgccc ggcgatcgcg gacccgttgc ccagtttatg tacgaaaacc
    11101 cgcagggaac gcggctgacg ctctacgtga gcccgccggc tgagaatgat cgcgaaagcg
    11161 cattccgctt tgccgacgag gcgggcgtcg gcgtcttcta ctgggtcgat ccgcgcttcg
    11221 cctacgcgct cgccggcgac ctcgatcgcg acacgctgct ggccattgcc gagaccgcct
    11281 atcaccagct cgaccgtgcc ggggccggac tctgagtccg cccgcactca ccacccgtag
    11341 gggcatcgcc gccgcgcaag gcacgctgat tacgctggcg ctgctgccgg cgatcatcat
    11401 gctggccggc gccagcccct tccccggcat cggcgagcgc gacgccgacc tgctgcgcga
    11461 tagcgggctc tcggcgctgc tgctcaccgt cctcgccacc gccgtcccga cgcttcgcca
    11521 gcgcctcggc ctgccctggc tgctgcgcct gcggcagccg atcggcgcac tcgccttcgc
    11581 ctatgccttg gcccacggac tgctggcaat gcgtgccgcc ggcggatcaa gggtcagtga
    11641 gctgctgcgc gccgcggtcg aggagccggc ccagctcgcc ggcctcgcct ccttcgcgct
    11701 ggcgctgccc ttgctcgcga cgagcaaccg cttggcgtta cgctggctta gcgcgcctgt
    11761 ctggcaggac gtacaacgca gtctgggaat cgtcgccgcg ctggcgacgg cgcacgcaat
    11821 cggcctggtc acggcaacac cgcgcctgat catcgtcctg ctggccgctg ccgcgctggt
    11881 ctggatcgcg ttcgccgccg cgcgttcgct gcgcgatcgg ctgcgccagc cggccacccc
    11941 gccggcggcg ccgggcgggc aagtgctgcg cttctaccgg cggcggccgc cgaggtaggc
    12001 accgaccgcc gcgcgaccct cggcgtcgag atgcaggcca agcttgctgc ggcgccagag
    12061 caggtcgtcg gcggtctgcg cccactcc
//
LOCUS       DATDAA010000091        16939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_356297,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000091 DATDAA010000000
VERSION     DATDAA010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 16939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16939
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356297"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            438..1100
                     /locus_tag="VIS73_03025"
     CDS             438..1100
                     /locus_tag="VIS73_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5762158.1"
                     /translation="MRLLLIEDDPMIGAGVQQALQREGYAIDWLQDATAALGAIEDHP
                     YELVLLDLGLPSGDGLELLRRLRAQGKTLPVLIMTARDSVADRVKGLDAGADDYLLKP
                     FDLAELGARIRVLLRRNREQADPLLRIGDLAIDPSTHGVSVAGEVVKVSAREFALLHA
                     LAEQPGRPLSRAQLEDRIYGWDEEVESNAVEVHIHALRRKIGAKRIANLRGVGYYLVD
                     KP"
     gene            1097..2458
                     /locus_tag="VIS73_03030"
     CDS             1097..2458
                     /locus_tag="VIS73_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5762159.1"
                     /translation="MTSLRRTLLITLLGAIALISGLNALATYQLARAGIDELMDYQLR
                     QLALSLRNQHLTRGFADPLVAPDEALDVVIQIWDVRGVRLYLSHPHTSLPSLVRLGYS
                     TAATAEGSWRVYAVPMPDHVIQVAQRLRVRAKLAARSALQTLLPTLATIPVLGVLIWL
                     LITRGLRPLQILTREVHHRQPEALAPIDSRSAPEEVRPLVDALNQLLSRLGRALDVQR
                     EFIADAAHELRTPLTALQIQLQLAESADDTAQRQNAHENLRGGLTRSIHLVNQLLTLA
                     RLEPGADDGKAAETHIALAALVRSALIDFAPLAAAREINLGATKLDEAAIVSGIDLDL
                     RALLANLISNAVKYTPAGGQIDVAVDASGPPPGYARLRVDDSGPGIPEAERGRVFDRF
                     YRRGAQGAPGASGTGLGLAIVQRIASRHRAEVNLGTAPLGGLRVEVRIPLAGNATDSP
                     APP"
     gene            2517..3974
                     /locus_tag="VIS73_03035"
     CDS             2517..3974
                     /locus_tag="VIS73_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY5762160.1"
                     /translation="MKSLSRKISALTLALTVVAAFAISHSSAATAQTRATAPAETRMP
                     TAGAARGLPNFADIVERYGPSVVHIRVEGQAGNRTPAANPFGNLDPNDPFFQFFRRFG
                     PPMQPPGGNQRPVRGLGSGFIVSADGLILTNAHVVADAAEITVRLSDKRELQAKLVGL
                     DKLTDIALLRIPAKGLPTIPIGDPAKARVGDWVLAIGSPFGFDNSVTAGIVSAKSRSL
                     PDENYVPFIQTDVAINPGNSGGPLLNLDGEVIGINSQIYSRSGGYQGLSFAIPIDVAE
                     QIKTQLLATGKVTRGRLGISIQGLNQGLAKSFGLDSVQGALVSQVEPDSPAAKAGVQV
                     GDVILEFNGDTVDDSSSLPPMVGSVKPGTKVKLKVWRERKEKTLTVTVGEMPNQSVAA
                     ADSPDGAQLGVAVRPLAKNEAANAQLEGGLLVLKVTGAAAQAGIEVGDIILAVNGAAM
                     ETVDDLRASLKGVGRHAAVLVQRGESRIFIPVDLG"
     gene            4022..4423
                     /locus_tag="VIS73_03040"
     CDS             4022..4423
                     /locus_tag="VIS73_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762161.1"
                     /translation="MKRTRISARARKHFLQLAAVAFAMGSEGMLVNAAEAAIVDERSG
                     PTLGTLTGDERYHHEFTLQRDPAGDLLSARVEVIPLARADAFTVVAAGACCIGPLQPG
                     PHALRITQGQQVDNHLIRIDRSTGAFLHFLA"
     gene            4595..5800
                     /locus_tag="VIS73_03045"
     CDS             4595..5800
                     /locus_tag="VIS73_03045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5762162.1"
                     /translation="MNEPSLRAADADIGIETDAVEIRVVTAAAEISAEDWQALGVPGH
                     PFLNRDFLDIVERTGVAGPEAGWQALHLAATVDSRVVGLLPLYLKSHSYGDFIHDWNW
                     SPVWERYGKRYYPKLVSGLPHTPVSGPRLLVAPHHPRPKTIRDLLLDAARQLVADNDF
                     SSWHVALGSEDEIAGLEARGLLVSHDVQFHWHDAGFGDFDGFLRSFSAEKRRKVRAER
                     RRAAESGLSIRVRHGHEIAREEWPALHAIYRSTFDRFGNYPAFTVDCFAELAAALNDR
                     MVVFSAHDGDDPVALSLCFRSDDTLFGRYWGALRSVDSLHFELCFYQGITYCLREGLS
                     RFEPGAGGEHKIARGFAPQIVRSAHWIADPGMRRIIAPYLARQQDGVGAYRDDAAAHL
                     PYRKSEASA"
     gene            5797..6423
                     /locus_tag="VIS73_03050"
     CDS             5797..6423
                     /locus_tag="VIS73_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEY5762163.1"
                     /translation="MNDKHRNAIPVVDKPQAPATERNRDPILAILKRQFADRQRVLEI
                     GSGTGQHAIHFAAAMPQLRWQCSDRPANLPGIAMWLDEAALPNTPPPIALDVNEPPAL
                     RERYDAVFSANTLHIMGWREVERLFALLPTLMADGALLTIYGPFNYDGCFTSESNAQF
                     DAGLRNDHPERGIRDFEAVDALAQAIGLRLIEDAEMPINNRCLSWRLD"
     gene            complement(6452..6802)
                     /locus_tag="VIS73_03055"
     CDS             complement(6452..6802)
                     /locus_tag="VIS73_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY5762164.1"
                     /translation="MSEIKRSGTTRRWSDAVAWQQLLFLCEVPGQLEADISEQTREVL
                     ATLDQRLGEAGSSRQRILNATIYLPSREDLDAFNAIWDEWVPAGHAPVRACVHAALTD
                     PRMRVEIQMVAAGA"
     gene            complement(6844..7254)
                     /locus_tag="VIS73_03060"
     CDS             complement(6844..7254)
                     /locus_tag="VIS73_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HEY5762165.1"
                     /translation="MESSPKSPRNVVLQTLRESFPAFASFQPLAIGIHEAVNARLPDL
                     VAAELKTALRIHTKSTRYLKSMATAKHRVDLDGNPAGDITDEQRAHAAELLKERFRKD
                     RERRDAEDAVRKAQEAEQRKQEKLQQLAARFSKR"
     gene            complement(7453..8241)
                     /locus_tag="VIS73_03065"
     CDS             complement(7453..8241)
                     /locus_tag="VIS73_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762166.1"
                     /translation="MNHTSIVIGALFAALAVSACAANAHTVRLAEINIVNRDTGERLT
                     PYHHGGRVYVAGTPGQRYSIELKNRDRGRLLSVLSVDGVNVLTGESAAPAQSGYVLSP
                     WQQYAITGWRKSADEVAQFVFTALPDSYAARTGRPDNVGVIGIALFREKAAPAPVELS
                     QAPASAPAHESMADSRAAEERQEQHGAKRKMRADRLGTGHGAREVSRTSWTDFERASA
                     APDELVTIYYDSRQNLIARGVIRVPHNPKPNPFPAFGFVPDPRG"
     gene            complement(8329..9018)
                     /locus_tag="VIS73_03070"
     CDS             complement(8329..9018)
                     /locus_tag="VIS73_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762167.1"
                     /translation="MSDKDLYENWRRAGREEPPASLDARILAAAHEAAGETKNPRRPS
                     WIARFAPVTVAAVAVLGVALSMRVADESPELRQSLPPPAAPAASSRAAPAAAPAEKTS
                     REFPAKAESRSDRLLRDDPAAASADARDSLELRGEAVSEAVPDADAAVPRRKRAAEAT
                     LQPRAPAAPAAKLGAAETAEPMDAASWLADIERLLAVGETERAQQSLQRFRIAHPGYP
                     LPESLTTLGTK"
     gene            complement(9015..9590)
                     /locus_tag="VIS73_03075"
     CDS             complement(9015..9590)
                     /locus_tag="VIS73_03075"
                     /inference="COORDINATES: protein motif:HMM:NF009166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY5762168.1"
                     /translation="MGDARTDEALMLAYRDGEAGAFDVLYGRHRAPVYRYLLRQCAGR
                     AQADEVFQEVWMAVIRARAGYEVVAKFTTWLYRIAHNKLVDHYRLSGRIGEYEAPMSE
                     VESGEAIALPDAAAETPEQVRLRQEVVVRLVEAVDALPLAQREAFLLAADGELSVEEI
                     ARATGVPFETAKSRLRYANARLRQLLVDLRR"
     gene            complement(9691..10455)
                     /locus_tag="VIS73_03080"
     CDS             complement(9691..10455)
                     /locus_tag="VIS73_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762169.1"
                     /translation="MQDARTTYVKTPKGLDEIEHKTNGLHLKARQVLIMIDGKRDIEA
                     LQGIFPPEMVPAVLEELLREGYVRELARPKAPPPPRPVPTAQLDGEDPFDLSQTFMIN
                     IARKILGIAGDEIVAKVEAARDLETLRGLFPEWRKAITQAPDGVMRIKEFEFKLFQVL
                     GEVSPNAAPATPEKPKNPPTNDDERFILARNFMVNTARNYLGIVADSFVPKVDDAQSL
                     ADLRHLFFEWREALRQSPDAKRRLAEYESKLAALLS"
     gene            complement(10641..10717)
                     /locus_tag="VIS73_03085"
     tRNA            complement(10641..10717)
                     /locus_tag="VIS73_03085"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10681..10683),aa:Pro,seq:ggg)
     gene            complement(10829..12085)
                     /locus_tag="VIS73_03090"
     CDS             complement(10829..12085)
                     /locus_tag="VIS73_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEY5762170.1"
                     /translation="MAKKKNAFYAQSGGVTAVINASAAGVIETARKHADVIGKVYAGR
                     DGIIGALTENLIDTSRESDAAIAALKSTPSGAFGSCRFKLKGLDQNRREYERLIEVFK
                     AHNIGYFFYNGGGDSADTCFKVSQLSETMGYPVQAIHVPKTVDNDLPITDCCPGFGSV
                     AKYVAVSALEASFDVRSMARTSTKVFVVEVMGRHAGWIAAAGGLLEAQGIPVVILFPE
                     IEFDEKKFLARVDDNVKRQGYCTVVVSEGCHYPDGRFLAEQGTRDAFGHAQLGGAAPV
                     VANMVKEALGYKFHWAVADYLQRSARHIASKTDLRHAYQVGKAAVELALKGRNAVMPT
                     IERISDSPYKYRIGVAELKNVANVEKFMPRDFISADGFGITAKCRRYLEPLIQGEDYP
                     RYANGLPQYVTLKNIAVAKKLPEFKL"
     gene            complement(12117..12794)
                     /gene="adk"
                     /locus_tag="VIS73_03095"
     CDS             complement(12117..12794)
                     /gene="adk"
                     /locus_tag="VIS73_03095"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY5762171.1"
                     /translation="MRLILLGGPGAGKGTQAQFIRERFGIPLISTGDMLRAEVRADSA
                     LGRRVKEVMDSGGLVSDELILDLVRARIDREDCAAGYLFDGFPRTIPQAEALRAAAVP
                     LDAVVEIAVDDAEIIRRMAGRRVHQASGRSYHIEFNPPRVAGRDDETGEPLVQRDDDR
                     EETVHERLKIYHQQTAPLVDYYRRWAASGDTAAPRCCNVAGAGTVESVRDRIAAALDA
                     CVADAAA"
     gene            complement(13035..13631)
                     /locus_tag="VIS73_03100"
     CDS             complement(13035..13631)
                     /locus_tag="VIS73_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5762172.1"
                     /translation="MVRDSYKALQPSPVKPATMCRHPRSFGMVRLDAPAIEVMTDFEE
                     VSPASISPDATLQEATAKMISRGVRLLFVARADDVIQGVITARDTMGEKPVKLLQERG
                     GRREDLHVADLMTPQSMMEALSMRDVLHAEVGHILQTLKQIGRQHALVVEFDPMTGRE
                     EIRGMFSATAIGRRLGVPIQTFEIANTFAEIEAALADS"
     gene            13974..14339
                     /locus_tag="VIS73_03105"
     CDS             13974..14339
                     /locus_tag="VIS73_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEY5762173.1"
                     /translation="MLAAITTLLVYQLVGEVISRVLELPVPGPVIGMALLFATLLLRE
                     SFAEPLRETAQTLLQHLSLLFVPAGVGIMLHFHRVGAEWLPLAVSLIVSTVLAIAVTA
                     LVLQLLARGSQTADREDVS"
     gene            14336..15079
                     /locus_tag="VIS73_03110"
     CDS             14336..15079
                     /locus_tag="VIS73_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEY5762174.1"
                     /translation="MNAELPQKFSEIWVYLSTTPLLGLTVTLLAYQGAYWIYRKADFH
                     PVANPVLWSVVALVALLLVTDTPYQTYFDGAQFVHFLLGPATVALAVPLHAQLPRLKR
                     LAMPLLAALAAGSLVAAFSAMAVGYMLGGSLATVLSLAPKSVTTPIAMGIAEKIGGLP
                     SLTAVLVISTGIIGAVLGRYVLDALRIKDHAVRGFSIGVAAHGIGTARAFQVSEQAGA
                     FAALAMGLNGLMTAVLLPALLPLVTPLLR"
     gene            complement(15095..16063)
                     /locus_tag="VIS73_03115"
     CDS             complement(15095..16063)
                     /locus_tag="VIS73_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5762175.1"
                     /translation="MAYRLPPLAALRAFESAARHGSFKDAATELSVTPAAVSQQIKLL
                     EDYLDAALFQRLSRGIQPTDAALAMLPKLREGFECLAAAVEATRSGSGFGALTVTAPP
                     SFAVRWLVPRLNSFQSAHPDCALNVASSLSTVDRRLGVASQGGEEVDPRSEETHISIR
                     FGHGRYPGYEVMRIFAPDYAPACSPSLPGESRPLAVPSDVRRFPLIHDETVPDLEDRP
                     TWESWLRLADVTGVDTHRGPRFNNQALAIEAAIAGQGLVLVPKALISADVQAGRLIVP
                     FDLEVPSRYAYYLVTPQAVSMRPAVRAFHDWLLQAVASDPVLAGHR"
     gene            16220..16429
                     /locus_tag="VIS73_03120"
     CDS             16220..16429
                     /locus_tag="VIS73_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762176.1"
                     /translation="MLNQETAAVVIARNRIDQAIARARRERDAEVARLLGLAFASVRA
                     AITRSIQRAGAAIRAAVVSGNAHHA"
     gene            16476..>16939
                     /locus_tag="VIS73_03125"
     CDS             16476..>16939
                     /locus_tag="VIS73_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEY5762177.1"
                     /translation="MDFLIEILLKAGRSGIELALFVLLPVMVVMLSLMRLLEARGVID
                     WLVNRLAPPLRPIGLTGLGVFAALQISFVSFAAPMATLAMMETRGASNRHIAATLAMV
                     MAMAQANVTIPMVTMGLGLGTTLFWSLLGGLVAAAATYHLFGRTLDAAEPVV"
BASE COUNT         2855 a         5792 c         5334 g         2958 t
ORIGIN      
        1 gaccttgccc aggcgatcga ggtccgacga aacgagtccc gccaatgcat tctcaagctg
       61 ctgttcgaca tagccgccca tctgcagcgc gttggtacgg atcgcgtcga gttccagttc
      121 gaattgccgg ctcaggtgtt cgttcatcat cttttgcatc gctcccgtat cccccggacg
      181 aaggcaccgc ccgtttcggc gccatgttat ttacgctgtg tgacggtttg atgaccgtac
      241 agcccgactg ccgccgatga tagcgccggc cgggcgccaa tgcggacgcg gctccggctc
      301 cggctccggc tcccaggccc gcagcgcgaa agcggcgaaa gcgcttggca gattgctcgg
      361 ccggggtttt cagtccggcg ccggacaacg cacaatagcc gccatgctcg ccacccacca
      421 gaccgtgcgc tgatccgatg cgcctcctgc tcattgaaga cgacccgatg atcggtgccg
      481 gcgtccagca ggcgctgcag cgggagggct acgccatcga ctggctgcag gatgccaccg
      541 ccgcgctcgg tgcgatcgag gatcatcctt acgagctggt gctgctcgat ctcgggctgc
      601 ccagcggcga cggccttgag ctgctgcggc gcctgcgggc gcaaggcaaa acgctgccgg
      661 tgctgatcat gaccgcccgc gattcggtgg ccgaccgggt caaggggctc gacgccggcg
      721 ccgacgacta cctgctgaag ccattcgacc tcgccgaact gggcgcgcgc atccgggtcc
      781 tgcttcggcg caaccgcgag caggcggacc cgctgctgcg gattggcgac ctggcaatcg
      841 acccgagcac ccacggcgtc agcgttgccg gcgaggtagt caaggtatcg gcgcgcgagt
      901 ttgcgctgct gcatgcactc gccgagcagc ccggccgacc gctgtcacgc gcgcagctgg
      961 aagaccgcat ctacggctgg gatgaggaag tcgaaagcaa cgcggtcgag gtgcacatcc
     1021 atgcgctgcg ccgcaagatc ggggcgaagc gtatcgccaa cctgcgcggc gtcggctact
     1081 acctcgtcga caagccatga cctcgctgcg ccgaaccctg ctgattaccc tgctcggcgc
     1141 catcgcgctg atctcggggc tcaatgccct tgccacctac cagctggcgc gggccggcat
     1201 cgacgagctg atggactacc agctgcgcca gctcgccctg tcgctgcgta atcagcacct
     1261 gacccgcggc ttcgccgacc cgctggtcgc gcccgacgag gcgctcgacg tcgtcatcca
     1321 gatctgggat gtgcgcggtg ttcgcctcta cctctcgcac ccgcatacct cgctgccctc
     1381 gctggttcgc ctgggttatt cgactgcagc gactgctgaa ggcagctggc gtgtctatgc
     1441 ggtgccgatg cccgaccacg tgatccaggt cgcgcagcgc ttgcgcgtac gcgcgaaact
     1501 tgcggcgcga tcggcgctgc agacgctgct gccgacgctg gcgacgattc cggtactggg
     1561 cgtcctgatc tggctgctga ttacccgcgg cctgcgccca ctgcagatcc tgacgcgcga
     1621 ggtgcaccat cgccagcctg aagcgctggc gccgatcgat tcccgctctg cgcccgaaga
     1681 agtacgaccg ctggtcgacg cgctcaatca gctgctgtcc cgcctggggc gggcgctgga
     1741 cgtgcagcgc gaattcatcg ccgacgccgc gcatgagctg cgcacgccgc tgaccgcttt
     1801 gcagatccaa ctgcagctcg ccgagtcggc cgacgacacg gcccaacggc agaacgcaca
     1861 cgagaacctg cgcggcggac tgacacgcag catccatctg gtcaatcaac tgctgacact
     1921 cgcgcgtctg gaacccggcg ccgacgacgg caaggcggcc gaaacgcaca tcgcgctcgc
     1981 ggcattggtg cgatcggcgc tgatcgattt cgcgccgctg gccgcggccc gggagatcaa
     2041 cctgggtgca accaagctcg acgaggccgc catcgtctcc ggcatcgacc tcgacctgcg
     2101 cgcgctgctc gccaacctga tcagcaatgc cgtgaagtac acgccggccg gtgggcagat
     2161 cgatgtcgcc gtcgacgcca gcggcccgcc acccggctac gcccgcctgc gtgtcgacga
     2221 cagcggcccc ggcattcccg aggccgagcg cgggcgcgta ttcgatcgct tctatcgtcg
     2281 cggcgcccag ggtgcaccgg gtgcatcggg aaccggcctc ggcctggcca tcgttcagcg
     2341 gatcgccagc cggcaccgtg ccgaggtcaa tctcggcacc gctccgctgg gcggcctgcg
     2401 cgtcgaagtg aggattccgc tggcgggcaa cgccacggac agcccggcgc caccttaagt
     2461 ctttcttaag tctggctgcc tagactcggc acatcgattc aatcaggagg tgtgtcatga
     2521 agtcgctgtc ccgcaagatc tctgcactga cgctcgcatt gacggtcgtc gccgcgtttg
     2581 ccatcagcca ctcctcggcc gccacggccc agacccgtgc cacggcgcct gccgaaaccc
     2641 ggatgccgac cgccggcgca gcccgcggcc tgccgaattt cgccgacatc gtcgagcgct
     2701 acggtccctc ggttgtccat atccgcgtcg aaggacaggc tggaaaccgc acaccggcgg
     2761 ccaatccctt cggcaacctg gatccgaacg atcccttctt ccagttcttc cgccgcttcg
     2821 gaccgccgat gcagccgcca ggcggcaacc agcgcccggt tcgtgggctt ggctcgggct
     2881 tcatcgtctc ggccgacggc ctgatcctga ccaatgcgca cgtcgttgcc gacgccgccg
     2941 aaatcaccgt ccgcctcagc gacaagcgcg aactgcaggc caagctggtc ggcctcgaca
     3001 agctgaccga catcgccctg ctgcgcatcc cggcgaaggg actaccgacg attccgatcg
     3061 gcgatccggc aaaagcacgc gtcggcgact gggtgctggc aatcggctcg cccttcggct
     3121 tcgacaacag cgtcacggct ggcatcgtct cggccaagtc gcgcagcctg cccgacgaga
     3181 actacgtccc gttcatccag accgacgtgg cgatcaaccc aggcaactcc ggcggcccgc
     3241 tgctcaacct cgacggcgag gtgatcggca tcaattcgca gatctacagc cgctccggcg
     3301 gttaccaggg gctctccttc gcgatcccga tcgacgtcgc cgagcagatc aagacccagc
     3361 tgctcgctac cggcaaggtc acgcgcggcc gcctcggcat ctcgattcag ggcctcaacc
     3421 aaggcctcgc caagtccttc gggctcgact cggttcaggg tgctctggtc agccaggtgg
     3481 aacccgacag tccggcggcc aaggcgggcg tccaggtcgg cgacgtcatc ctggagttca
     3541 acggcgatac ggtcgatgac tcgtccagcc tgccgccgat ggtcggctcg gtgaagcctg
     3601 gcaccaaggt caagctcaag gtctggcgcg agcgcaagga gaagaccctg accgtgacgg
     3661 tcggcgaaat gcccaaccag tcggtggctg cggccgactc gccggacggt gcgcagcttg
     3721 gtgtcgcggt ccggccgctg gcgaagaacg aggcggccaa cgcccagctc gagggcggat
     3781 tgctggtact caaggtcacc ggcgcggcgg cgcaggctgg cattgaggtc ggcgacatca
     3841 tcctcgcagt caatggcgcc gcgatggaaa ccgtcgacga cctgcgcgcc tcgctcaaag
     3901 gcgttggtcg ccacgcagcc gtgctggtcc agcgcggcga gtcacgcatt ttcattccgg
     3961 tagacctcgg ttgagctcgg ctgagcggcc gaatcctcag tcatcgagac aggagacttc
     4021 catgaaacgc acacgcatct cggcccgggc gcgcaaacac tttctgcagt tggcggcagt
     4081 ggccttcgcg atgggcagcg agggcatgtt ggttaacgcc gccgaagcag cgatcgtcga
     4141 tgagcgatcg ggtccgacgc ttggcaccct caccggcgac gagcgctatc accatgaatt
     4201 cacgctccag cgagaccccg ccggcgacct gctgagcgcc cgggtcgagg tgatcccgct
     4261 ggcccgtgcc gatgccttta ccgtggtcgc ggccggcgcc tgctgcatcg gcccgctgca
     4321 acccggaccg cacgcactga ggatcaccca gggccagcag gtcgacaacc atctgatccg
     4381 catcgaccgc agtaccggcg ctttcctgca cttcctcgcc tgacgccgcg gtgcggcgcc
     4441 gagcacgccg gtcggcacgc ggcgcggccg gagggtgccg gtggcgcaag ggatgatcag
     4501 gtcgcgcgca ggcagtcgga acgaattgcc gtgccgttgt ccaaactcgg cccttgccaa
     4561 accggccgac ccgactgcaa cccgcgccac gaccatgaac gaaccgagcc tccgcgctgc
     4621 cgatgccgac atcggcattg aaaccgacgc tgtcgaaatc cgcgtcgtca ccgcagcggc
     4681 cgaaatcagt gccgaagact ggcaggcgct cggcgtacct gggcacccct tcctcaaccg
     4741 cgactttctc gacatcgtcg agcgtaccgg ggtcgctggg cccgaggccg gctggcaggc
     4801 gctgcatctg gcagccaccg tcgactcccg cgttgtcggg ctgctgccgc tctacctgaa
     4861 gagccactcc tacggtgatt tcatccacga ctggaactgg agcccggtct gggagcgcta
     4921 tggcaagcgc tactacccga aactggtctc ggggctgccg cacacgccag tgtccggccc
     4981 acgcctgctg gtcgcccccc accatccgcg gccgaaaacg attcgcgatc tgctgctcga
     5041 cgcggcacgg cagctcgtcg ccgacaacga tttttcgagc tggcacgtcg cgctcggcag
     5101 cgaagacgaa atcgccggcc tcgaagcccg cggcctgctg gtcagccacg acgtgcagtt
     5161 ccactggcac gacgccggct tcggcgactt cgacggcttt ctccggtcgt tttccgccga
     5221 gaagcgccgc aaggtccgcg ccgaacgccg gcgcgccgcc gaatcggggt tgagcatccg
     5281 tgttcgccac ggtcatgaga ttgcgcgcga ggaatggccc gcgttgcacg cgatctaccg
     5341 ctcgaccttc gatcgctttg gcaactaccc ggcgtttacc gtcgactgct ttgccgagct
     5401 ggcggcggcg cttaacgacc gcatggtggt attcagcgcg cacgacggcg atgatccggt
     5461 ggcgctttcg ctctgcttcc gcagcgacga cacgctgttc ggccgctact ggggggcgct
     5521 gcgcagcgtc gatagcctgc atttcgaact ctgcttctat cagggcatca cctactgcct
     5581 gcgcgaaggg ctctcccgct tcgagcccgg tgccggcggc gaacacaaga tcgcccgcgg
     5641 cttcgcgccg cagatcgtcc gttcggcaca ctggatcgcc gaccccggaa tgcggcggat
     5701 cattgcgccg tacctcgcgc gacagcagga cggcgtcggc gcctaccgcg acgacgcggc
     5761 ggcacacctg ccctaccgca aatcggaggc aagcgcgtga acgacaagca ccgcaacgcg
     5821 atacccgtcg tcgacaaacc gcaggccccg gcgaccgagc gcaatcgcga tccgatcctg
     5881 gccatcctga aacgccaatt cgccgaccgc cagcgcgtcc tcgaaatcgg cagcggcacg
     5941 ggtcagcatg cgattcactt cgcggcggcg atgccgcagc tacggtggca atgctcggac
     6001 cgccccgcca acctgccggg aatcgcaatg tggctcgatg aggcggcact accgaacacg
     6061 ccgccgccga tcgccctcga cgtaaacgag ccgccagcgc tgcgcgaacg ctacgacgcc
     6121 gtgttcagcg ccaacacgct gcacatcatg ggttggcgcg aagtcgaacg gctctttgcc
     6181 ttgctgccga cgctgatggc cgacggcgcg ctgctgacga tctacggccc gttcaactac
     6241 gacggctgtt tcaccagcga gagcaacgcg cagttcgatg ccgggctccg aaacgatcac
     6301 cccgaacgcg gcattagaga cttcgaagcc gttgatgcgc tggcgcaggc aatcgggctg
     6361 cgcctcatcg aagacgccga gatgccgatc aacaaccgct gcctaagctg gcgactagac
     6421 tgacgccgac tcggcggcac cccgatttcg gtcaggcgcc cgccgccacc atctggatct
     6481 cgacgcgcat ccgcgggtcg gtcagcgccg cgtgcacgca cgcgcgcacc ggcgcatgac
     6541 cggccggaac ccattcgtcc cagatcgcgt tgaacgcatc gaggtcctcg cgcgacggca
     6601 gataaatcgt cgcgttgaga atccgctgcc ggctgctgcc cgcttcgccc aagcgctggt
     6661 ccagcgtcgc gagcacttcg cgggtctgct cggaaatgtc ggcctcaagc tgacccggaa
     6721 cctcgcagag aaacagcagc tgctgccagg ccaccgcatc cgaccagcgg cgtgtcgtgc
     6781 cggagcgttt tatctcactc atgccagcag cctttccgca aaacgttacg ccattaccgg
     6841 caatcagcgc ttgctgaagc gtgccgcgag ctgctgcagc ttttcctgct ttctctgctc
     6901 ggcttcctgc gccttgcgta ccgcatcttc ggcgtcacga cgctcacggt ctttgcggaa
     6961 gcgctccttg agcagttcgg cggcgtgtgc gcgctgttcg tcggtgatgt cgccggccgg
     7021 gttgccatcg agatcgacgc ggtgcttcgc cgtcgccatc gatttcaggt aacgcgtcga
     7081 cttggtatga atccgcaggg cggttttcag ctcggcagcg acgaggtcgg gcaggcgcgc
     7141 attgaccgct tcgtggatgc cgatggccag cggctggaag gatgcgaatg ccggaaagct
     7201 ttcgcgcagg gtctgcaaca cgacgttgcg gggggatttc ggcgacgact ccatgcgcgt
     7261 tcgattccgg tgtcggttct ttggaaaacg gcgcacttca gagacgccgc ggcatgcgtt
     7321 tctggtaccg ccgctctgcc gcgccccagt ccgcaggcgg caggatgcca caaactgaag
     7381 aagcccatgg aaccaccaac ggccgacacc gcgctttcca cggtgacagc cgttgattcg
     7441 ggcggcaatc ggctagccgc gcggatcggg gacgaagccg aaggccggaa acggattggg
     7501 cttcgggtta tgcggcacgc ggattacgcc gcgcgcgatc aagttctgcc gcgagtcgta
     7561 gtagatcgtc accagctcgt cgggcgccgc gctcgcccgc tcgaaatcgg tccagctcgt
     7621 gcgcgagacc tcgcgggcgc cgtgcccggt accgagccga tcggcgcgca tcttgcgctt
     7681 cgccccgtgt tgctcctggc gctcctcggc cgcgcgcgaa tcggccatcg actcgtgcgc
     7741 cggcgcagat gccggcgcct gggataactc gactggcgca ggcgcggcct tctcgcggaa
     7801 cagcgcgatg ccgatcacgc cgacgttgtc gggacggccg gtgcgcgcgg cgtagctgtc
     7861 cggaagcgcg gtgaaaacga actgcgcgac ctcgtcggcc gacttccgcc agccggtgat
     7921 cgcgtactgc tgccatggac tcagcacgta gcccgattgc gccggcgcgg ccgattcgcc
     7981 agtcagcacg ttgaccccat cgaccgacag caccgagagc agtcggccgc ggtcgcggtt
     8041 tttcagctcg atcgaatagc gctgccccgg cgtgcctgcg acgtacacac ggccgccgtg
     8101 atggtatggc gtcagccgct cgccggtgtc gcggttgacg atgttgatct cggcgagcct
     8161 gaccgtatgg gcgtttgccg cacaggccga aaccgcgagg gcggcaaaca gcgcgccgat
     8221 gacaattgac gtgtggttca tgttcgttct cccgacagtg aatgcgatgt ccgcactcct
     8281 gcaatcgaac gagcacgcga aacggggtta aaccctttgc atcgatgcct attttgtacc
     8341 gagcgtggtc agggattcgg gcagcggata acccggatgc gcgatccgga aacgctgcag
     8401 cgactgctgc gcccgctcgg tttcgccgac ggccagcagg cgctcgatgt cggccagcca
     8461 gctggcagca tccatcggtt cagccgtttc ggccgcgccc agctttgccg caggagcagc
     8521 cggcgcgcgc ggctgcaggg tcgcctcggc cgcgcgcttg cgacgcggca ccgccgcatc
     8581 ggcatcgggc acggcttccg acactgcttc gccgcgcaac tccaatgagt cccgcgcgtc
     8641 cgccgatgcg gccgcggggt cgtcgcgcag caaacgatcg ctgcggcttt ccgccttggc
     8701 aggaaattcg cgcgacgtct tctcggcggg tgcggcagcc ggcgctgctc tcgacgaagc
     8761 cgccggagca gccggtggcg gaagcgactg cctgagttcc ggcgattcat cggccacccg
     8821 catgctcagc gcgacgccga gcactgccac ggcggcaacc gtgaccggcg cgaagcgggc
     8881 gatccaggag gggcgccgcg gatttttcgt ttcgccggcc gcctcgtgcg ccgcggcaag
     8941 aatgcgcgcg tcgaggctgg ccggtggctc ttcacggccg gcgcggcgcc agttttcgta
     9001 caggtccttg tcgctcatcg tcgcaggtcc accagcagtt gccgcaagcg cgcgttggcg
     9061 taacgcagcc ggctcttggc cgtttcgaaa ggcacgcccg tggcgcgggc gatctcttcc
     9121 accgaaagct cgccatcggc ggcaagcaga aaggcttcac gctgcgccag aggcagcgca
     9181 tcgaccgcct cgaccagacg caccacgacc tcctgccgca accgcacctg ctcgggcgtc
     9241 tccgccgccg catcaggcag cgcgatcgcc tcgcccgact cgacctcgga catcggcgcc
     9301 tcgtactcgc cgatccggcc acttaaccga tagtggtcga cgagcttgtt gtgcgcgatc
     9361 cggtacagcc aagtcgtgaa cttcgccacc acctcgtagc ccgcgcgtgc ccggataacc
     9421 gccatccaga cctcctgaaa cacctcgtcg gcctgcgcgc gcccggcgca ctggcgcagc
     9481 aggtagcgat agaccggtgc ccggtggcgg ccgtagagca cgtcgaaggc acccgcctcg
     9541 ccgtcgcggt aggcgagcat cagcgcctcg tcggtacgag cgtcacccat agactgttaa
     9601 cggtgcaatc gtgggaacgg ggtcatcgcc ggcggcgcag gtcgcgcaat ccaagcgcaa
     9661 gcatcgaatc cgcgggctac cgcccacgcg tcacgaaagg agcgccgcga gcttcgactc
     9721 gtactcggcc agtcgccgct tggcgtccgg cgactgccgg agcgcctcgc gccactcaaa
     9781 gaacaagtgg cgcaggtcgg ccagcgattg cgcgtcgtcg accttcggca cgaaggaatc
     9841 ggcgacgatt ccgaggtagt tgcgcgccgt gttgaccatg aagttgcgcg ccaggatgaa
     9901 gcgctcgtcg tcgttggtcg gcgggttctt cggcttctcg ggcgttgccg gcgccgcgtt
     9961 gggcgacacc tcgccgagta cctggaacag cttgaactcg aactccttga tccgcatcac
    10021 gccgtccggc gcctgggtaa tcgccttgcg ccactccggg aacagcccgc gcagggtttc
    10081 aagatcgcgt gccgcctcga ccttggcgac gatttcatcg ccggcaatgc cgaggatctt
    10141 gcgcgcgatg ttgatcatga aggtctgcga cagatcgaat gggtcttcgc catcgagttg
    10201 agccgtcggc accggacgcg gaggcggtgg cgccttgggt cgcgcgagtt cgcgcacgta
    10261 gccttcgcgc agcagttctt cgagaaccgc cggaaccatc tccggcggga agatgccctg
    10321 caaggcttct atgtcgcgtt tgccgtcaat catgatcagc acctgacgcg ccttgagatg
    10381 caggccattc gtcttgtgct cgatctcgtc gagtcccttc ggcgtcttga cgtaggtcgt
    10441 ccgggcgtcc tgcattgttc tgttgtctcc ccaccccggc tctgcggccg gacgagtact
    10501 tcttaagtca tagcattcta tcggcgtgac tgacgcgggg cttacaagcg ccgcttcatt
    10561 gttactcgcg atccctccgc tatcaagcga ggaaggcaaa aaggccagtc ttttgactgg
    10621 cctttttgcc tcatttccaa tggtcggggc ggcgggattc gaactcgcga ccccttgcac
    10681 cccatgcaag tgcgctacca ggctgcgcta cgccccgacc gaggcgcgga ttatagcgac
    10741 attcgccggg cggaaccacc ggactttcgc caatgtagca agaaaaaaag ggaccccgat
    10801 tgcaccggca ccttcccgca aacccgcatc aaagcttgaa ctcgggcagt ttcttggcga
    10861 ccgcgatgtt cttcagcgtg acgtactgcg gcaggccgtt ggcgtatctg ggatagtcct
    10921 cgccctggat caatggctcc agatagcgac ggcacttggc ggtaatgccg aagccgtcgg
    10981 cgctgatgaa atcgcgcggc atgaacttct cgacgttggc gacgttcttc agttcggcga
    11041 cgccaatacg gtacttgtag ggcgagtcgg aaatccgctc gatggttggc atgacggcgt
    11101 tgcggccctt aagtgccagt tcgacggcgg ccttgcctac ctggtaggcg tggcgcagat
    11161 cggtcttcga ggcgatgtgc cgcgccgagc gctgcaggta gtcggcgacg gcccagtgga
    11221 acttgtagcc cagcgcctcc ttgaccatgt tggcgacgac aggcgcggcg ccgccgagct
    11281 gtgcatggcc gaacgcatcg cgcgtgccct gctcggcgag gaatcgccca tcgggatagt
    11341 gacagccctc ggagacgacg accgtgcagt agccctggcg tttgacgttg tcgtcgacac
    11401 gggcgaggaa cttcttctcg tcgaactcga tttcgggaaa aaggatgacg accggaatcc
    11461 cctgcgcttc cagcaggccg ccggcggcgg cgatccaccc ggcgtgccga cccatgacct
    11521 cgacgacgaa gaccttggtc gaggtccgcg ccatcgagcg cacgtcgaag gaggcttcga
    11581 gcgccgatac cgcgacgtac ttggcgaccg agccaaagcc ggggcagcag tcagttatcg
    11641 gcaggtcgtt gtcgacggtc ttaggcacat ggattgcctg caccgggtaa cccatggttt
    11701 ccgacagctg cgagaccttg aagcaggtat cggccgaatc gccaccgccg ttgtaaaaga
    11761 agtagccgat gttgtgcgcc ttgaaaacct cgattagccg ctcgtattca cgccggttct
    11821 gatcgagccc cttgagcttg aagcggcagg aaccgaaggc gcctgatggc gtgctcttca
    11881 gcgccgcgat ggcggcatcg ctctcgcggc tggtgtctat gaggttctcg gtcagcgcgc
    11941 cgatgatgcc gtcgcgcccc gcgtagacct tgccgattac gtcggcatgc ttgcgcgccg
    12001 tctcgatgac gccggcagcc gatgcattga taacggcggt gacgccgccg gactgcgcat
    12061 agaaggcatt cttcttcttc gccattgttt ctccctggcc gaatggacct cgccgctcag
    12121 gccgctgcgt cggcgacgca ggcatcgagc gcggcggcta tcctatcgcg caccgactcg
    12181 acagtgccag ccccggcgac attgcagcag cgcggcgcgg cggtatcgcc acttgccgcc
    12241 cagcgccgat agtagtcgac cagcggcgcg gtctgctggt gatagatctt caagcgttca
    12301 tgcaccgtct cttcgcggtc gtcgtcacgc tgcaccagcg gctcgcccgt ttcgtcatcc
    12361 ctgccggcga cgcgcggcgg gttgaactcg atgtggtagc tgcgtcccga agcctgatgc
    12421 acgcgacgac cagccatgcg gcggatgatc tcggcgtcgt cgacagcgat ctcgacaacc
    12481 gcgtcgagcg gcacggcggc cgcacgcagc gcttccgcct gtggaatcgt gcgcggaaaa
    12541 ccatcgaaca ggtaaccggc cgcacagtct tcgcgatcga tccgcgcgcg gaccagatcg
    12601 aggatcagtt cgtcggaaac gagaccgccg gaatccatga cctccttgac ccggcgaccg
    12661 agcgccgaat cggcccgcac ttcggcccgc agcatgtcgc cggtagaaat cagcgggatg
    12721 ccgaagcgct cccgaatgaa ctgcgcctgt gtccccttgc ccgcgccggg gccgcccaac
    12781 agaatcaatc gcatggtgct gtctcctggt ctcctgagtg aatgaccgac agcgcgcgtt
    12841 ttcccggatc gaaccgcctc ctcgcctgcc ggtgcctgtc gcgtgtcgct tctggcgagc
    12901 ggcatcgcgg ccaacagtct aggcgccaat ccaattaagt aaaatttact tgttcttgcc
    12961 gaaacggtta gcgattcatg accaacaaaa aagggcggcc gacgccgccc cttacctgcc
    13021 gagaagacag ctcgtcagct atccgccagt gccgcctcga tttcggcgaa ggtattggcg
    13081 atttcgaatg tctggatcgg cacgccaagc cggcgaccga ttgcagtggc cgagaacatg
    13141 ccgcggattt cttcccgacc ggtcatcgga tcgaattcga caaccagcgc gtgctggcga
    13201 ccgatctgct tcaaggtctg cagaatatgt cccacctcgg cgtgcagaac gtcccgcatc
    13261 gacaaggctt ccatcatgct ctgcggcgtc atcaggtcgg ctacatgaag atcttcacga
    13321 cgaccgccgc gctcctgaag caacttgacc ggcttctcgc ccatcgtgtc gcgggcggta
    13381 atcacgccct ggatgacatc gtcggcacgc gccacgaaca acagccgcac gccgcgcgag
    13441 atcatcttgg cagtagcctc ctgcaaggtc gcgtcgggac tgatgctcgc aggcgagacc
    13501 tcttcgaagt cggtcataac ctcgattgca ggcgcatcga gccgaaccat gccaaagctg
    13561 cgcggatgcc ggcacatcgt ggcgggcttg accggcgagg gctgcaaagc cttgtaggaa
    13621 tctctaacca tcgtctcctc ctgtcctatt ggttttgtct gccgtatcga tccgcacccg
    13681 gcctggatcg ctgacggtga tcgcgccgcg taacgggcgg atacgtctaa ctctacgccg
    13741 tatccgcccg aacgcaacac ctgccgcttc tcctctgcag tcatcgcgac gggtcgcacc
    13801 ggtctgcgcc aatggcaacc gcgtcgagtc tattgtgggc ggcgcagcgg cgacagttaa
    13861 tcctccttca ccttcgggtt agcgattgct tgcgagaccg atcggcaaac gacttgcatc
    13921 atgccgcgat ccattcaatc atcggtcgcg ttcacaaccc aaggagtcag gcgatgctcg
    13981 ctgcgatcac cacgctgctg gtctatcagc tcgtcggcga agtcatcagc cgcgtgctcg
    14041 aactgccggt tcccggcccg gtcatcggca tggctttgct gttcgcgacc ctgttgctcc
    14101 gcgagtcctt cgccgagccc ctgcgcgaaa ccgctcagac cctgttacag catttgtcgc
    14161 tgctattcgt cccggcgggc gtcggcatca tgctgcactt tcatcgcgtc ggcgccgagt
    14221 ggctgccgct tgcggtttcg cttattgtca gcacggtgct ggcaatcgcg gtgacggcgc
    14281 tggtcctgca actgctggcg cgcggttcgc aaacggccga ccgggaggac gtctcgtgaa
    14341 cgccgaactg ccacagaagt tctcggagat ctgggtctac ctgtcgacga cacctttgct
    14401 cgggttgacg gtgaccctgc tcgcctacca gggtgcgtac tggatctatc gcaaggccga
    14461 cttccatccg gtggccaatc ccgtgctctg gtcggtcgtg gcgctggtgg cgctgctgct
    14521 ggtgacagac accccatacc agacctattt cgacggtgcg cagttcgtac acttcctgct
    14581 cggaccggca accgttgccc tcgccgtgcc gctgcacgcc cagctgccgc ggctgaagcg
    14641 gctggcgatg ccgctgctcg cggcgctggc cgccgggtcg ctggtcgccg ctttttcggc
    14701 gatggcggtc ggctatatgc tcggcggcag tctggcgacg gtgctgtcgc tggcgccgaa
    14761 gtcggtgacc acgccgatcg caatgggcat agccgaaaag atcggcggac tgccatcgct
    14821 gaccgcggta ctggtaattt caacgggcat catcggcgcc gtgcttggcc gctacgtgct
    14881 cgacgcgctg cgcatcaagg accacgccgt gcgcggcttc tcgatcggcg tcgcggcgca
    14941 cggcatcggc accgcacggg cctttcaggt cagcgaacag gcgggcgcct tcgccgcgct
    15001 ggcgatgggc ctgaacggcc tgatgaccgc ggtgctgctg ccggccctgc tgcctttggt
    15061 cacaccgctg ctgcgctagg ccaacgcctc gatctcagcg atgtcccgcc agcaccggat
    15121 cgctggcaac cgcctgcagc agccaatcgt ggaaggcgcg tacggccgga cgcatcgaca
    15181 ccgcttgggg ggttaccaga tagtaggcgt aacgcgacgg cacctcgagg tcgaagggca
    15241 cgatcaggcg acccgcctgc acgtcggcgc tgatcagcgc cttgggcaca aggaccagtc
    15301 cctggccggc aatcgcggcc tcgattgcca gcgcctgatt gttgaagcgc ggcccgcggt
    15361 gggtatcgac gccggtcacg tcggccaaac gcaaccagga ttcccaggtc ggtcggtcct
    15421 cgaggtcggg caccgtttcg tcgtgaatca agggaaagcg acggacgtcg gaaggcacgg
    15481 ccagcggcct gctctcgcca ggcaacgagg ggctgcaggc cggcgcgtag tccggcgcga
    15541 agatccgcat cacctcgtat cccggatacc gcccgtgccc gaagcgaatg ctgatgtggg
    15601 tttcctcgct gcgcgggtcg acctcctcgc cgccctgtga agcaacgccc agacgccggt
    15661 ccaccgtgct caggctgctc gcgacattga gcgcgcaatc gggatgcgca ctctgaaagc
    15721 tgttgaggcg cggcaccagc cagcgcaccg cgaacgatgg cggcgcggtc accgtcagcg
    15781 cgccgaaccc gctgccactt cgggttgcct cgacggcagc ggcgaggcac tcaaagcctt
    15841 cgcgcagctt gggcagcatt gccagcgccg catctgtggg ctggatgccc cgggaaagcc
    15901 gctgaaacaa cgcggcatcg aggtaatcct cgagcaactt gatctgctga ctgaccgcgg
    15961 ccggggtcac gctcagttcg gtcgctgcat ccttgaagct gccgtgcctg gcggccgact
    16021 caaaggcgcg cagggctgcc agaggcggta atcggtaagc catattaaat cctcacacat
    16081 tagacctgct aatgctgcgg atcataacaa atcgtttgct gcgttgccgc agtccacgaa
    16141 taatgccgtc catggtcggc aaacgagaaa aaccgacccg gatcggcagc cccgctaggc
    16201 tgcgcaatag gagtaagaaa tgcttaatca agaaaccgcc gccgtcgtta tcgcccgcaa
    16261 ccggattgac caggccatcg cccgcgcccg ccgcgaacgc gacgccgaag tcgcccgcct
    16321 gctcggcctc gccttcgcaa gcgtccgcgc cgcaattacc cgcagcatcc agcgtgccgg
    16381 cgccgcgatc cgcgccgccg tcgtcagcgg aaacgcacac cacgcctgac ccagcaggag
    16441 tttgtgcggc gcagcgtcaa tgagacaatg cgccgatgga cttcctgatc gagattctgc
    16501 tcaaggcggg ccgctccggc atcgagctag cgctgttcgt gctgttgccg gtgatggtag
    16561 tgatgctgtc gctgatgcga ctgctcgagg cccgcggcgt catcgactgg ctggttaatc
    16621 gcctggcgcc accgctgcgg ccaatcggcc tcaccggcct tggcgtcttc gccgccctgc
    16681 agatcagttt cgtcagcttc gcggcaccga tggcgacgct ggcgatgatg gaaacgcgcg
    16741 gcgcctcgaa tcgccacatc gccgcgacgc tggccatggt gatggcgatg gcgcaggcca
    16801 acgtcacgat ccccatggtg accatgggcc tcgggctcgg caccaccctc ttctggtcgc
    16861 tgctcggcgg cctcgtcgcg gcagccgcga cctaccatct attcggtcga acactcgacg
    16921 ccgccgaacc ggtcgtcga
//
LOCUS       DATDAA010000092        11115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_356317,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000092 DATDAA010000000
VERSION     DATDAA010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11115
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356317"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VIS73_03130"
     CDS             complement(<1..505)
                     /locus_tag="VIS73_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5762178.1"
                     /translation="MVRDQGAPSPTFSPLYRQIKDLMVDRLQAGEWLPGDSIPSEAEL
                     AGRFGVSQGTVRKAIDEMAAENLLVRRQGKGTFVASHADPRSSFRFLRLAHDGGEIMP
                     PRSIPLECWRAKAGADVARSLGLEAGAPIIIVRRMLKFADKPTVIDEIYLPGELFGDL
                     SLEVLRSF"
     gene            698..1687
                     /locus_tag="VIS73_03135"
     CDS             698..1687
                     /locus_tag="VIS73_03135"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY5762179.1"
                     /translation="MSKAPVRVAVTGAAGQIAYSLLFRIASGEMLGKDQPVILQLLDL
                     PQAQKAVGGVMMELDDCAFPLLQGMVATDDPNVAFKDAEACLLVGARPRGPGMERRDL
                     LTANGAIFTVQGKAIAENAKENVRVLVVGNPCNTNALIAASAAKKVGRTDPRNYHGMV
                     RLDHNRAMSMAAAKAGRAVSSLTKMIVWGNHSPSMYADYRFCTSNGDSVKKLINDDQW
                     NNDVFLPTVGKRGAAIIEARGLSSAASAANAAIDHMHDWFLGSDDWVTMGVPSDGSYD
                     IPKGIVYGFPCECKNGDYKIIQGLEIDAYSREKMNKTLAELEEERAAVAEMLK"
     gene            1758..2753
                     /locus_tag="VIS73_03140"
     CDS             1758..2753
                     /locus_tag="VIS73_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY5762180.1"
                     /translation="MRDPDSVLFSGEKPLPSLPAVDHYCGSEKLIRKALALQQQTGPV
                     FDITCDCEDGAAAGQEREHVTMCAALIASDDNRFGRAGLRVHDATHPAWRTEIEIALQ
                     KAGTRLPYLTLPKARSAADVATQIEFLRRTERALGLQRSIPVHVLIETHGALREVWQI
                     AGLAGVESIDFGLMDFVSDHLGAIPGSAMKSPGQFEHPLLVRAKAEIVAAGLANGVVP
                     THNVTTELRDKELIRDDARHARSGFGFLRMWSIHPNQIEPILDAFRPDFSETEEAGCI
                     LAKAQDADWGPIQEAGRLHDRASFRYYWSLLKRARQTGAALPEPIVSRFFSRPQG"
     gene            complement(2750..3181)
                     /locus_tag="VIS73_03145"
     CDS             complement(2750..3181)
                     /locus_tag="VIS73_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762181.1"
                     /translation="MAEKSGATSASKQAADSPVTHAVSGSPWHWDVGALWHQTIISQQ
                     QWLLEWWLGLISGALQPARLASVTGTEARQRLAFAHRQLGCVGAADLADADARVSGGE
                     RSSAVAMPPPRASAEKSGSGAVACAMPRRRRRVPNPNPNPS"
     gene            3357..4808
                     /gene="gabD"
                     /locus_tag="VIS73_03150"
     CDS             3357..4808
                     /gene="gabD"
                     /locus_tag="VIS73_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY5762182.1"
                     /translation="MKLKNTDLLRSQAFIDGRWIDADDGQRLDVSNPANGNLVGNVPL
                     MGSAETRRAILGADAALSAWRSRTAADRARILRGWFERIMANQEDLALLMTTEQGKPL
                     AEARGEVAYAASFIEWFAEEGKRVYGDTIPATQADRRILVIKQPIGVCAAITPWNFPL
                     AMITRKAGPALAAGCTMVIKPASQTPLSALALARLAEEAGIPPGVLSVVTGSATAIGG
                     EMTANPVVRKLSFTGSTEIGIELMRQCADTVKKLSLELGGNAPFIVFDDADLDAAVAG
                     ALASKYRNSGQTCVCANRLLVQDGVYDSFVDRLAATVAQLKVGDGLDPQTTQGPLIDE
                     RAVAKVEAHIADALAKGARVALGGKRHHLGGNFFEPTILADVTTEMAVTREETFGPLA
                     PVFRFRDENDAIAMANDTEFGLAAYFYSRDVGRVFRVAEALEYGIVGANTGLISTEVA
                     PFGGIKASGIGREGSHYGIEEYLEVKYICLAGI"
     gene            4850..5215
                     /locus_tag="VIS73_03155"
     CDS             4850..5215
                     /locus_tag="VIS73_03155"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEY5762183.1"
                     /translation="MPSPKGATGLRRIVRAAAYSAAGLRYAYQHEAAFRQELLLLAIL
                     APVALWVSDDAITRVLMIGSLVLVIIVELLNSAIEATIDRISPERHSLAKAAKDIGSA
                     AVMIALFLSALIWGILLLS"
     gene            5265..6947
                     /locus_tag="VIS73_03160"
     CDS             5265..6947
                     /locus_tag="VIS73_03160"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5762184.1"
                     /translation="MVKFSAVKRLVFSVRSRVAVFALAATILPSATLGWLSYQRNAEV
                     LAQKTAQELDAGASVAAREIDLWMKDRTEEMRIFGSSYLITENLERLAALEKGGDASM
                     RRQVTSRMRDYLASVTSRFPGFAGLRVVAPDGSVIYSSHRDPVLNLPEHWLRATASGR
                     VVIGEPYWSAGDESTIVGIAVPVRSGRGQALGALVASVNAKAIGEILSRSARDATRIH
                     LIDHGGRPIGPGMPATTEAGVEPHGFQTAAFREYRNALGIDVVGVFRPVAEQVWGVVA
                     ERDRDLVFFEVNELRNTTFKLLLLLLVAVGIGAYWIGQTIVKPLDRLIAAANRVATGD
                     LDLTVPIQQRDELGHLTERFNEMTGQLRRNREELASANDELIRKNIELEAISITDSLT
                     SLYNRRHMMNVLEREFQQVARNGQTFTLMMIDLDHFKAINDSYGHPAGDAVLVTAARI
                     LRDAVRGVDYAARFGGEEFIVLLTDTGADEAEASAERIRSRLADGTISHGDDRITVTA
                     SIGLAEVIGAGDTPGALIARADAALYEAKQQGRNRVCRHQAKVHILHKSGKA"
     gene            complement(6975..7739)
                     /locus_tag="VIS73_03165"
     CDS             complement(6975..7739)
                     /locus_tag="VIS73_03165"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY5762185.1"
                     /translation="MHQCSRVIRSFASLALLAGLVAACAGLPQENGAAAAAMAAEKAA
                     QSEADRAQASRIAALQLELVVMAADNQRLLQAIEQGRKQLDEAMLEVVRSKAKLGSLG
                     SRAEAATALAESESLAKTLQSRSASIPQESEYRAAERMLALGNQEFKNGNFGGAYYLA
                     SNARTGFAVLVTRSATAVPPALADRPESRFAAPIDLVFSTRGTVRENPNTDARVIRVA
                     DKGTEVTGLAARDLWILVEFRDGSSGWAFYDLVAAP"
     gene            7903..8811
                     /locus_tag="VIS73_03170"
     CDS             7903..8811
                     /locus_tag="VIS73_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY5762186.1"
                     /translation="MLILGSRRFRSLNRVLDFEQTGDYAFHTNTTTITPEETFGMSVK
                     VGTPDDLPKSVVPSVMRAARLLDAVASASEPLSLTALRRQLALPKSSTLSLCTSLTST
                     GLLRRSEEGTYHLGSRLVDLAHSYLRRTDLPSEFAQLWSSSDPLSDESGVLAVLDGAD
                     VIYIACRNGSRPHGLSFRVGMRLPATCTATGKTMLAALPDQHVRTLLSASSLKSLTPH
                     SHCDIDTLLADLARVRQRGYGVDDEETLEGTYCVGVPITDCGRAPAAVAVSFVKGSNF
                     EERARTLIPRLQEIATRLAPHVSMLL"
     gene            8918..10087
                     /locus_tag="VIS73_03175"
     CDS             8918..10087
                     /locus_tag="VIS73_03175"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY5762187.1"
                     /translation="METETKTVTTKSAEIESTAPKQKNRRRFLTAAAAGTGAAITGFP
                     MIAVAQSPVVFKMQGAWGAKDIFNEMAEEYVKRVNEMAGGRMRIDYLVAGSVVKPFEV
                     MDAVNKGVLDAGHHVPVYWYGKSKVASLFGSGPINGCDAQQTLAWIYRGGGYELYQEL
                     LTKLQFDIVGYFCMPMPTQPLGWFKKPVKTAADMKGLKYRTVGLAADVMQEMGLKVTQ
                     LPGGEIVPAMERGVIEAFEFNNPTSDRRFGAQDVAKNYMMGSFHQAMEFFEIVFNKKK
                     HDALPADLKAILRYAAEAASSSNWWTAMDNYSRDLDELQTKNGVNVMRTPKSIFEEQL
                     KAWDTVNARLSAEDPFFKKVVDSQRAWSKRVAKYWFLNDADYKLGYEHIHGKLGF"
     gene            10174..10782
                     /locus_tag="VIS73_03180"
     CDS             10174..10782
                     /locus_tag="VIS73_03180"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEY5762188.1"
                     /translation="MFIEGAIHTIDRLSRGVGHAFAWCIVFLTLGTTYEVFVRYVLRD
                     PTSWAYDMSYILYGALFLMSGAYALSRNAHVRGDIIFRLLKPRVQAWLELVLYFFFFF
                     PGVIALMYSGWGYAADAIRLTEVSVNSPVGVPIWQLKAIIPAAGALLALQGIAEILRC
                     VVCIRSGEWPPRLHDVEELESALKAQQEDLARQQNKAKAKKA"
     gene            10779..>11115
                     /locus_tag="VIS73_03185"
     CDS             10779..>11115
                     /locus_tag="VIS73_03185"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY5762189.1"
                     /translation="MSDPHVALLMLGLFIFVILLGFPIAFTLMAMGVAFGYYAYFTPD
                     QAFFDNRVFYLLTQNTFSVMNNDVLVSIPLFLFMGYIIERAAILDKLFYSLQVAFRRV
                     PGAMAVAALV"
BASE COUNT         2168 a         3620 c         3336 g         1991 t
ORIGIN      
        1 cgaaactgcg tagcacttca aggctgagat cgccgaacag ttcgccgggc agatagatct
       61 catcgatgac tgtcggcttg tcggcgaatt tgagcatgcg gcgaacgatg atgatcggcg
      121 cgccggcctc aaggccgagc gagcgagcga catcggcgcc cgccttcgca cgccagcatt
      181 cgagcggaat gctgcgcggt ggcataattt cgccgccgtc gtgggcgagc cggagaaaac
      241 ggaaggagga tcgcgggtcg gcgtgcgaag cgacgaaggt acccttgccc tgccgccgaa
      301 ccaacaggtt ctcggcggcc atttcatcga tcgccttgcg gacggtaccc tgactgaccc
      361 caaaacgtcc ggccaactcg gcctcgctcg ggatagaatc gccgggcaac cactcgccgg
      421 cctggagacg gtcgaccatc agatccttga tctgacgata aagcggactg aacgtcggcg
      481 agggagcacc ttggtcgcgt accatcggcg aaattagagc acagttttgg ggtctcgtct
      541 attccacctt ctatcttata acttatataa gatagatatt gacaccggcc gacccgctgc
      601 ctaagatgca tcggctcggc ttgaaccagc cgcctctcgc atcgtcgaga ccgctcgtcg
      661 atacgtccac acatcaaacc accgacagga gcccctgatg tccaaagccc ccgttcgcgt
      721 cgccgttacc ggtgccgccg gccagattgc ctacagcctg ttgttccgca ttgccagcgg
      781 cgaaatgctt ggcaaggacc aaccggtcat tctgcagttg ctcgacctgc cgcaggccca
      841 gaaggctgtt ggcggcgtga tgatggaact cgacgactgc gccttcccgc tgctgcaggg
      901 catggtcgcg accgacgacc cgaatgtcgc cttcaaggat gccgaagcct gcctgctggt
      961 cggcgcccgt ccccgcggcc ccggcatgga gcgtcgcgac ctgctgacgg ccaacggcgc
     1021 catcttcacc gtccagggca aagcaatcgc cgaaaacgcc aaggaaaacg tccgcgtact
     1081 ggtcgtcggc aacccctgca acaccaacgc cctgatcgct gcctctgccg caaagaaagt
     1141 cggtcgcacc gacccccgta actaccacgg catggtccgc ctagaccaca accgcgcaat
     1201 gtcgatggcc gccgctaagg ccgggcgcgc cgtttcgtcg ctgaccaaga tgatcgtctg
     1261 gggcaaccac tcgccgtcga tgtacgccga ctatcgtttc tgcacgtcga acggagattc
     1321 ggtcaagaag ctgatcaacg acgaccagtg gaacaacgac gtcttcctgc cgaccgtcgg
     1381 caagcgcggc gccgcgatca tcgaagcgcg cggcctgtct tcggctgctt cggctgccaa
     1441 cgccgcaatc gatcacatgc atgactggtt cctcggctcc gacgactggg tcacgatggg
     1501 cgtcccctcg gatggttcct acgacatccc gaagggcatc gtctacggct tcccttgcga
     1561 gtgcaagaat ggcgactaca agatcattca gggtcttgag atcgacgcct acagccgcga
     1621 gaagatgaac aagacgctgg ccgaacttga ggaagagcgc gcggccgtcg ccgaaatgct
     1681 caagtaacgt cggcgcaatc cgtcaacgag ggccgcggta gaatgccgcg gccctttttc
     1741 attgcctcca cgctcccatg agagacccag actcggtgct gttctcgggc gaaaagcccc
     1801 tgccctcgct gcccgccgtc gatcactact gcggctcgga aaagctgatc cgcaaggcgt
     1861 tggccctgca gcaacaaacc ggcccggtat tcgacatcac ctgcgattgc gaggacggtg
     1921 ccgcggctgg acaggaacgc gagcacgtca cgatgtgcgc ggcgctgatc gcctccgacg
     1981 acaaccgctt cggccgggcc ggactcaggg tccacgacgc cacgcatccg gcatggcgaa
     2041 ccgaaatcga gatcgcgctg cagaaagccg ggacgcggtt gccgtacctg acgctgccca
     2101 aggcgagaag cgccgccgat gtcgccaccc agatcgaatt cctgcggcgg accgaacgcg
     2161 cgcttggcct tcagcgttcg atccccgtgc atgtgctgat cgaaacgcat ggcgcgctgc
     2221 gcgaagtctg gcagatcgcc ggcctcgccg gtgtcgagtc gatcgacttc ggcctgatgg
     2281 atttcgtctc cgaccatctg ggcgcgattc cggggtcggc catgaaaagc cccggccagt
     2341 tcgagcatcc ccttctggtt cgcgccaagg cggagatcgt cgccgcggga ttggccaacg
     2401 gggtggtgcc gacccataac gtgaccaccg aactgcgtga caaggaactg attcgcgacg
     2461 acgcgcgcca cgcacgttcg ggtttcggct tcttgcgtat gtggagcatt caccccaacc
     2521 agatcgaacc gatcctcgac gcctttcgcc ccgacttctc cgagaccgag gaggcgggct
     2581 gcattcttgc caaagcacag gatgccgact ggggaccgat tcaagaagcc ggccgactgc
     2641 acgaccgtgc atcattccgt tactactggt cgctgctcaa gcgcgcgcga caaaccggcg
     2701 ctgcacttcc cgagccgatc gtttcgcgct tcttcagccg cccccagggc taactagggt
     2761 tcgggttcgg gttcggcacc cgccgtcgac gacgaggcat ggcgcaagcg accgcccccg
     2821 aaccggattt ctcagcactt gcccgcggcg gcggcatcgc gacagcggat gaccgttctc
     2881 cacccgaaac gcgggcgtcg gcatcggcaa ggtcggcggc accgacgcac ccgagctggc
     2941 ggtgcgcgaa cgccagacgc tgccgcgctt cagtgccggt caccgacgcc aggcgcgctg
     3001 gttgcaaggc ccccgagatc agaccaagcc accattcgag cagccactgc tgctgagaga
     3061 taatcgtctg gtgccataag gcgcccacgt cccaatgcca cggcgatcct gagacggcat
     3121 gggtcaccgg cgagtccgcc gcctgtttgc tcgcactcgt tgcgccagac ttttcggcca
     3181 tcagacacct cctgaatgaa gcacctattc aatcgggcgc catcggcaac cccggaacca
     3241 tagcggcacg gccacccgca atgcctgcta gcatgcccat tatttgaatt tctgagggaa
     3301 gcgcaagctg gccggcagat ttcaccgcgc cacccaatca cgccgacgac gaaatcatga
     3361 aactgaaaaa caccgacctc ctccgctcac aggcgttcat cgacggccgc tggatcgacg
     3421 ccgacgacgg gcaacgcctt gatgtcagca atcccgccaa cggcaatctc gttggcaacg
     3481 tcccgctgat gggttctgcc gaaacccggc gggcgattct cggagccgac gccgcgcttt
     3541 ccgcctggcg cagccggacg gccgccgacc gagcccgcat actgcgcggc tggttcgaaa
     3601 gaatcatggc caaccaggaa gatctggcgc tgctgatgac caccgaacag ggcaagcctc
     3661 tcgccgaagc gcgtggcgaa gtcgcttacg cggcgtcctt catcgagtgg tttgccgagg
     3721 agggaaaacg tgtctacggc gacacaatcc cggccaccca ggcagaccgt cgcatcctcg
     3781 tcatcaaaca gccgatcggc gtctgtgcgg cgatcacccc gtggaacttc ccgctggcaa
     3841 tgatcacgcg caaggcaggg cctgcccttg ctgctggctg cacgatggtc atcaagccgg
     3901 catcacagac accgctctcg gcgctggcac tagcccggct cgccgaggag gctggcattc
     3961 cacccggagt gctgagcgtc gttaccggtt ccgccaccgc gatcggtggc gagatgaccg
     4021 ccaacccagt ggtccgcaag ctgagcttca ccggatcgac cgagatcggc attgagctga
     4081 tgcgccagtg cgccgacaca gtgaagaagc tctcgctcga actcggtggc aatgcgccct
     4141 tcatcgtctt cgatgatgcc gatctcgatg ccgcggtagc cggcgcgctg gcatcgaagt
     4201 accggaactc gggacagacc tgcgtctgtg caaaccgcct gctggttcag gatggcgttt
     4261 acgacagctt tgtcgatcga ctcgccgcaa ccgtcgcgca actgaaggtc ggcgacggcc
     4321 tggacccgca gacgacgcag gggccactga tcgacgaacg cgccgtcgcc aaggtcgaag
     4381 cccacatcgc cgatgcgctc gccaagggcg cgcgcgtcgc cctcggtggc aaacgccacc
     4441 accttggcgg caatttcttc gaacccacga tcctcgccga cgtcacaacg gaaatggctg
     4501 tcacccgtga ggaaaccttc ggaccattgg cgccggtgtt ccgctttcgc gacgaaaacg
     4561 acgcgatcgc gatggccaac gatacagagt tcggactggc tgcctatttc tactctcgcg
     4621 atgtcggccg cgtgttccgg gtcgcggaag cacttgagta cggcattgtc ggcgccaata
     4681 ccgggctgat ctcgaccgaa gttgccccct tcggcggcat caaggcatcg ggaatcggcc
     4741 gcgagggttc gcactacggc attgaggaat acctagaggt caagtacatc tgccttgccg
     4801 gcatatgaac cggaacgcca cggaaactcc aacgggcgca gccgccgacg tgccatcgcc
     4861 caagggcgcg accggattgc gccgcattgt ccgggccgcg gcttactcgg ctgccgggct
     4921 tcgctacgcg taccagcacg aagcggcgtt tcgccaggaa ctgctgctgc tagctatcct
     4981 tgcgccggtc gccctctggg tcagcgacga tgccatcaca cgcgttctga tgattggcag
     5041 cctcgtgctg gtaatcatcg ttgagctgct caattcggca atcgaggcaa cgatcgaccg
     5101 gatttctccc gagcggcact cgctggccaa ggcggccaag gacatcggca gcgccgctgt
     5161 catgatcgcg ctgttcctgt cggcgctgat ctggggtatc ttgctgctat cctgaagctc
     5221 cgcaacccgg cacgaacgaa gtggttcggg ccgagaggtg ttctatggtg aagttttccg
     5281 ccgtcaaacg tctggttttc agcgtgcgca gccgggtcgc ggtctttgcg ctggcagcga
     5341 ccattctgcc ctcggcgacg cttggctggc tttcctatca gcgcaatgcc gaagtcctgg
     5401 cccagaagac cgcgcaggaa ctcgatgccg gcgcctccgt cgcggcgcgc gaaatcgatc
     5461 tgtggatgaa agatcgcact gaagagatgc gcatcttcgg cagctcctat ctgatcaccg
     5521 agaatctcga acggcttgcc gcgctggaaa aaggcggcga cgccagcatg cgcaggcagg
     5581 tcacctcgcg catgcgcgat tacctggcgt cggtgaccag tcgttttccc ggattcgcgg
     5641 gcctgcgggt cgtcgcgccc gacggctcgg tgatctacag ctcgcatcgc gatccggtgc
     5701 tcaatctccc cgagcactgg ctgcgcgcga ccgcgtcggg acgggtcgtt atcggcgaac
     5761 cgtactggag cgccggtgac gaatcaacca tcgtcggcat cgccgttccg gttcgctcgg
     5821 gccgcggtca ggccctagga gcactggtcg cctctgtcaa cgcgaaggcg atcggcgaga
     5881 tcctttcccg atctgcacgt gatgccaccc gaattcacct gatcgaccac ggcggccggc
     5941 cgattggccc gggaatgcca gcgacgactg aggccggtgt agaaccgcat ggattccaga
     6001 ctgccgcgtt ccgcgaatac cggaatgcac taggcatcga cgtcgtcggc gtctttcggc
     6061 cggttgccga gcaggtctgg ggcgtggttg ccgaacgcga ccgtgatctg gttttttttg
     6121 aggtcaacga actgcgcaac acgactttca aactactgct gctgctgctg gtcgcggttg
     6181 gaatcggcgc ttactggatc ggccagacga tcgtgaagcc gctcgatcgt ctgatcgctg
     6241 cagccaaccg ggtggcgacc ggtgatctcg acctcacggt gccgatccag cagcgcgacg
     6301 aactcggaca cctgaccgaa cgcttcaatg aaatgaccgg tcagctccgg cgaaaccgcg
     6361 aggaattggc cagcgccaac gacgaactca tccgcaagaa tatcgaactc gaagcgatat
     6421 cgatcaccga ttcgctgacc agtctgtaca accgtcggca catgatgaac gtgctcgaac
     6481 gcgagtttca acaggtcgcc cggaacggtc agacatttac gctgatgatg atcgacctcg
     6541 atcacttcaa ggcgatcaac gatagctacg gccacccggc tggagacgcc gtgctggtta
     6601 ccgctgcgag gatcctccgc gacgccgtcc gcggcgtgga ctatgcggcg cgctttggcg
     6661 gcgaggagtt cattgtcctc ctgaccgaca ccggggcaga cgaagcggaa gcgagcgccg
     6721 agcggatccg ttcacgcctg gccgacggca cgatcagcca cggcgacgat cggatcaccg
     6781 tcaccgcgag catcggactg gccgaggtca tcggtgcggg cgacacaccg ggcgccttga
     6841 tcgcgcgcgc cgatgcggcg ctgtatgagg cgaaacagca gggaagaaac cgcgtctgtc
     6901 gccatcaggc gaaggtccac atactgcata aaagcggaaa agcctgaacg ctccgacgat
     6961 gtacccgcgt tccctcaggg tgccgcgacc agatcgtaga aggcccagcc gctcgaccca
     7021 tcgcgaaact cgacgagaat ccacaggtcg cgagccgcca acccggtgac ttcggttccc
     7081 ttatcggcta cgcggatgac gcgcgcgtcg gtgttcgggt tttcgcggac agtgccgcgc
     7141 gttgaaaaga ccagatcgat cggtgcggca aagcgcgatt cggggcggtc cgctagcgcc
     7201 ggcggcaccg ccgtcgcgct gcgggtaacg agaacggcga agccggtccg cgcattgctc
     7261 gccagataat aggcgccgcc gaaattcccg ttcttgaact cctggttgcc gagcgccagc
     7321 atgcgttcag ccgcacgata ttctgactcc tgcgggattg aggccgagcg cgactgcagc
     7381 gtcttcgcca gcgattcgct ctcggccagg gccgtcgcgg cttcggccct gctcccgagg
     7441 cttcccagtt tcgccttgct gcgcacgacc tcaagcatcg cctcatcaag ttgctttcgg
     7501 ccctgctcaa tggcctgcag cagccgctga ttgtccgccg ccatgaccac cagttcgagc
     7561 tgcaacgcgg caatccgact tgcctgcgca cgatctgctt cgctctgtgc cgccttttcc
     7621 gcagccattg ccgctgctgc agcgccgttt tcctgcggaa ggccggcaca agcagccacc
     7681 agcccggcaa gcagggcgag cgaagcgaag ctgcggatga ctctcgaaca ttggtgcaag
     7741 gcgacctcca gccagcagga atggacgggc caacgagcgg ccctcaaggc cacgacgatg
     7801 gattttccac gttccactgt caagccggaa ccgcacgcgt gaaacgtaac agccaaaacg
     7861 caccgagccg ctccatcagc gcggattcgg ccgcgaaaag cgatgctcat tctgggaagt
     7921 cgacggttcc gctcgctgaa ccgggttctt gattttgaac aaactggcga ctacgctttt
     7981 cacactaaca cgacaacaat aacgccggag gagacattcg gcatgtcggt caaagtaggc
     8041 acaccggacg acctgccgaa gtcggttgtt ccatcggtaa tgcgcgcggc acggctcctt
     8101 gacgccgtgg cgagcgcgag cgaaccgctg tcgctgacag ctttgcgccg ccagctcgcg
     8161 ctgcccaaga gttctacact cagcctgtgc acgagcctga cgagcaccgg cctgctccgt
     8221 cgttcggaag aaggcaccta tcatctgggc tcacgtctcg ttgacctggc gcactcctac
     8281 ctgcggcgca ccgacctacc ctccgaattt gcccaactgt ggtcgagctc cgacccgctt
     8341 tcggacgaaa gcggcgtcct cgccgtgctt gatggcgcgg acgtcatcta catcgcgtgc
     8401 cgcaatggca gccgaccgca cggattgagc ttccgggtcg gcatgcgcct gccggcgacc
     8461 tgcacggcca ctggcaagac catgctcgcg gcgctgcccg atcagcatgt ccgtacgctg
     8521 ttgtcggcat cctcactgaa gagcctgacg ccccacagcc attgcgacat cgacacactg
     8581 ctcgccgatc tggcgcgcgt caggcaacgc ggctatgggg tcgatgacga ggaaaccctg
     8641 gaaggcacct actgcgtcgg cgttccgatc accgattgcg gaagagcgcc ggcggcggtc
     8701 gcggtgagct tcgtcaaggg aagcaatttt gaggagcgcg cccgcacgct gattccgagg
     8761 ctgcaggaaa ttgccacgcg gctcgccccg cacgtgagca tgctgctatg aaaggcattc
     8821 gagaatccgc cctcgggcgg tgacacaagg caatcgcaac ccgcttcccg agacgcgatt
     8881 gaatgttgag caacaaccac aggaggagac cataaagatg gaaacagaaa ccaagaccgt
     8941 aacgacaaag agcgccgaga tcgaatccac ggcgccgaag cagaagaacc gccgccgctt
     9001 tctgacggca gctgccgcag gtaccggcgc ggcgatcacc ggatttccga tgatcgcggt
     9061 ggcgcaatcg ccggttgtct tcaagatgca gggcgcgtgg ggtgccaagg acatcttcaa
     9121 cgagatggcc gaggagtacg tcaagcgcgt caacgagatg gccggtgggc gaatgcgtat
     9181 tgactatctg gtcgccggct cggtggtcaa gccgttcgag gtgatggacg ccgtcaacaa
     9241 aggcgtcctc gatgccggac accacgtacc ggtctattgg tacggcaaga gtaaggttgc
     9301 ctcgctcttc ggctccggcc cgatcaacgg ctgcgacgcc cagcaaacgc tggcatggat
     9361 ctaccgcggc ggcggctacg agctctacca ggaactgctt accaagctgc agttcgacat
     9421 tgtcggctac ttctgcatgc cgatgccgac gcagccgctg ggctggttca agaagccggt
     9481 caagacggca gccgacatga agggtctgaa gtatcggacc gttggcctcg ctgccgacgt
     9541 catgcaggag atgggcctca aggtcacgca gttgccgggc ggcgaaatcg ttccggccat
     9601 ggagcggggc gtcatcgagg ccttcgagtt caacaacccc acctcggacc gccgcttcgg
     9661 cgcacaggac gtggccaaga actacatgat gggcagcttc caccaggcga tggagttttt
     9721 tgaaatcgtc ttcaacaaga agaagcacga cgccctgccc gccgacctca aggcaatcct
     9781 gcgctatgcc gccgaggccg cttcgtcgtc gaactggtgg acggcgatgg acaactactc
     9841 gcgtgacctt gatgagttgc agaccaagaa cggcgtcaac gtcatgcgca cgccgaagtc
     9901 gatcttcgaa gagcagctga aggcgtggga caccgtcaat gcacgcctct ccgccgagga
     9961 cccgttcttc aagaaggtcg tcgacagcca gcgtgcgtgg tcgaagcggg tcgccaagta
    10021 ctggttcctc aacgacgcgg actacaagct cggatacgag cacatccacg gcaagctcgg
    10081 cttctgatac gagccagccg ttcaagctga aattgccgaa ccgaccggag ggccctggtg
    10141 gctctccggc cttccttccg gattggggct cccatgttca tcgaaggtgc catacatacg
    10201 attgaccgcc tgagccgcgg cgtcggccac gcctttgcgt ggtgcatcgt gtttctgacg
    10261 ctcggaacca cctacgaggt cttcgttcgc tacgtgctga gggacccgac cagctgggcc
    10321 tacgacatga gctacatcct ctacggcgcg ctattcctga tgtccggcgc ctacgcgctg
    10381 tcgcgcaatg cacacgtacg cggcgacatc atcttccgtc tcctcaagcc acgtgttcag
    10441 gcctggctcg agttggtgct ttacttcttc ttcttcttcc cgggcgtcat cgcgctgatg
    10501 tactcgggat ggggttacgc ggccgacgcg attcgcctaa ccgaggtttc ggtcaacagc
    10561 ccggtcggcg taccaatctg gcaactcaag gcaatcattc ccgccgctgg agccttgctc
    10621 gcgctgcagg gaatcgccga gatcctgcgc tgcgtagtct gcatccgcag cggcgaatgg
    10681 ccgccgcgcc tgcacgacgt cgaggaactc gagagtgcgc tcaaggccca gcaggaagac
    10741 cttgcacgtc aacaaaacaa agcgaaagcg aagaaagcat gagtgatccg cacgttgccc
    10801 tgctaatgct gggcctgttc atcttcgtca tcctgctcgg ttttccgatc gcgttcacgc
    10861 tgatggcgat gggtgtcgcc tttggctact acgcgtactt cacgccagat caggccttct
    10921 tcgacaatcg cgtcttttac ctgttgacgc aaaacacctt ctcggtgatg aacaacgatg
    10981 tgctggtctc gataccgcta ttcctgttca tgggttacat cattgagcgc gcggccattc
    11041 tcgacaaact gttctatagc ctccaagtgg ccttccgccg cgtgcccggc gcgatggccg
    11101 tcgccgcact ggtga
//
LOCUS       DATDAA010000093         2129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_76573,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000093 DATDAA010000000
VERSION     DATDAA010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76573"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="VIS73_03190"
     CDS             complement(<1..1574)
                     /locus_tag="VIS73_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY5762190.1"
                     /translation="MAAESYDAIVVGSGISGGWAAKELTEKGLEVLLLERGKNVEHIK
                     DYVNATKAPWEYPHRGGRTVAMEEAYPVLKRDYPLNEKNLDWWASDRDSPYTELKRFD
                     WYRGYHVGGRSLMWGRQSYRLSDFDFEANAREGIAVDWPIRYRDLAPWCDHVERHAGI
                     SGSLEDMPQLPDGEFQPAMPLNCGEELIAGRLARHFDGRRRIIPGRVANMTRPLPGRS
                     ACQYRNACWLGCPFGAYFSTQSSTLPAAMATNRLTLMPFAIVTELTYDRDRQRATGVQ
                     VMDAVTGKSTEFRAPVIFLCASALNSTWILMRSATDLWPDGLGSSSGELGHNLMDHHF
                     RIGARGIIEDESLGDKYYFGRRPTGFYIPRYRNLFGDKRDYLRGFGYQGSASRLDWER
                     AVAELGIGGDFKDRMAEPGEWRIGATAFGEMLPDHDNKVGLDASRKDKWGLPVLEFDC
                     EMGENEALMRIDMANDMAEMLDAAGVSRVETYEDEYYPGMGIHEMGTARMGRDRRTSV
                     LNAWNQVWDAPNVFVT"
     gene            complement(1663..>2129)
                     /locus_tag="VIS73_03195"
     CDS             complement(1663..>2129)
                     /locus_tag="VIS73_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY5762191.1"
                     /translation="QPENRQTIDDYKRWADVANRLGEACSRAGMRAAYHNHEFEFQAL
                     QGVEPFELLLAETDPELVYFELDFFWARFADQGVLDVIGRAPERFVMSHIKDMDAERN
                     MVSVGAGTIDFAAILADPVADSIRHCFVEHDNPEDPFRSAAFGHYSLKSILG"
BASE COUNT          341 a          697 c          703 g          388 t
ORIGIN      
        1 tcggtgacga atacgttcgg cgcgtcccac acctggttcc aggcgttgag caccgaggtc
       61 ctgcggtcgc ggcccatgcg cgccgtgccc atctcgtgga tgcccatgcc gggatagtat
      121 tcgtcctcgt aggtctcgac ccggctgacg ccggccgcat ccagcatttc cgccatgtca
      181 ttggccatgt cgatgcgcat cagcgcctcg ttctcgccca tctcgcagtc gaactcgagc
      241 accggcaggc cccatttgtc tttgcgcgag gcgtcgagac ccaccttgtt gtcgtgatcg
      301 gggagcatct cgccgaaggc cgtcgcgccg atgcgccact cgccaggctc ggccatgcgg
      361 tccttgaagt cgccgccgat gccgagttcg gcgacggcgc gctcccagtc gagccggctc
      421 gcgctgccct ggtagccgaa gccgcgcagg tagtcgcgct tgtcgccgaa caggttgcga
      481 tagcgcggga tgtagaagcc ggtcggacga cgtccgaagt agtacttgtc gccgagggac
      541 tcgtcctcga tgatgccgcg cgcgccgatg cggaaatggt ggtccatgag gttgtgtccc
      601 agttcccccg aactcgaccc gaggccgtcg ggccacaggt cggtggccga gcgcatcagg
      661 atccaggtgg aattcagcgc ggaggcgcag aggaagatca ccggcgccct gaactcggtg
      721 gacttgcccg tgaccgcgtc catgacctgc acgcccgttg cgcgctgacg gtcgcggtcg
      781 taggtcagtt cggtaacgat cgcgaagggc atcagcgtca ggcgattggt cgccattgcc
      841 gccggcagcg tcgaggactg cgtgctgaag taggcgccga aggggcagcc gagccagcag
      901 gcattgcgat actggcaggc ggagcggccc ggcaggggac gggtcatgtt ggcgacgcgg
      961 cccgggatga tgcgccggcg gccgtcgaag tgcctggcaa ggcgcccggc gatcagttcc
     1021 tcgccgcaat tgagcggcat cgccggctgg aactcgccgt cgggcagctg cggcatgtct
     1081 tcgagcgatc ccgagatccc ggcatggcgc tccacgtggt cgcaccaggg cgccagatcc
     1141 cggtagcgga tcggccagtc gacggcgatg ccctcccgcg cgttggcctc gaaatcgaag
     1201 tcgctcaggc ggtagctctg ccgcccccac atcagcgagc gaccgccgac gtggtagccg
     1261 cggtaccagt cgaagcgctt gagttcggtg tagggagaat cgcggtctga cgcccaccag
     1321 tccagattct tctcgttcaa agggtaatcg cgcttgagga ccgggtaggc ttcctccatc
     1381 gccaccgttc gccccccgcg gtgcgggtat tcccagggcg ccttggtggc gttgacgtag
     1441 tcctttatgt gctcgacgtt cttgccccgc tcgagcagca ggacctcgag ccccttctcc
     1501 gtcagttcct tggccgccca tccaccggag attccggatc cgacgacaat cgcatcgtag
     1561 ctttcggctg ccatgctgct ccccgcctgt tgttgttatc gaagcgtcac actcatgata
     1621 cggcccggcg cgggtcaatg cagcccgccc tgatcgccgg cctcacccga ggatcgattt
     1681 caaactgtag tggccaaagg ccgccgagcg aaacgggtcc tcggggttgt cgtgctcaac
     1741 gaagcagtgc cggatcgaat ccgcgaccgg gtcggcgagg atggcggcga aatcgatcgt
     1801 gccggcaccg acgctgacca tgttgcgctc ggcgtccatg tccttgatgt gcgacatcac
     1861 gaaccgctcg ggcgcccggc cgatgacgtc gagaacgccc tggtccgcga atcgcgccca
     1921 gaagaaatcg agctcgaaat acaccagctc gggatcggtc tccgcaagca ggagttcgaa
     1981 gggttccacg ccctggagcg cctggaattc gaattcgtgg ttgtgatagg cggcccgcat
     2041 gccggcgcgg ctgcaggcct cgccgagacg attggccacg tcggcccatc gcttgtagtc
     2101 gtcgatcgtc tgccggttct cgggctgta
//
LOCUS       DATDAA010000094         3250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_391472,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000094 DATDAA010000000
VERSION     DATDAA010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391472"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1928)
                     /locus_tag="VIS73_03200"
     CDS             complement(<1..1928)
                     /locus_tag="VIS73_03200"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY5762192.1"
                     /translation="MKRWIKAILVLLVGLLLLIVAVIVAAVLLFDGERVKAVLEESIA
                     EKTGRTLQIAGVPELAIWPRLGVRLGAVSLSEKLGSAEFAGFDSANLAVAVLPLFAGE
                     LVADKVSVAGLRARLVRGEDGTLNVADLVGAPGTAGAAPAAPVEESAKPPIRFDIGGI
                     HIDGRELSWHDRVAKQKFALRDFEIVTGRLGSGASGELRVGAELRMEAGASEEVLRVE
                     LASRYRMGDQELAFEALSGTVGGRAGGIAQLQLKLGAASVRYVFNGPIAELRDLLLDF
                     SGEVAGLTTAATVRSPSLSWRGAGAADGVLQIPQLVLTTQAVRGPGWKADAALAGGLE
                     MNPGMLLRLSGWKGDAGLTDSTLLQKPLKLATEFSADLDLNGPRGKVDLQFSLDESKS
                     RVRTTLNALSPLDLVADAQLGKLDFDAYLLPPPAEASAPPANAVGKADLDPPVDLSPL
                     AGKKVRARIESAGLKARGIVIDKALIEARLANGRLDLSPLQLAFYGGTLDGAASAVAA
                     GNAVNTRVKLTGVDLNPLLRDLASTDLLAGRADVVADLAARGATVGAMKRALAGDLQL
                     AVRNGALKGVNLGRSFRDLKAKFSQGATSEVAVQSDAQTDFSELTASFKVRDGVLRGD
                     DLAAKSPFLRLGGSGEVS"
     gene            complement(2054..3139)
                     /locus_tag="VIS73_03205"
     CDS             complement(2054..3139)
                     /locus_tag="VIS73_03205"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY5762193.1"
                     /translation="MPAQITTGTMFGCAIIGLLLTAAMVWITEYYTGTEYAPVKHVAA
                     ACQTGHATNIIAGIGISMKATALPVLSVCASIYAAFELGGLYGLAIAATSMLSMAGIV
                     VALDAYGPITDNAGGIAEMAELPAEVRNVTDPLDAVGNTTKAVTKGYAIGSAGLAALV
                     LFADYTHGLEAAGATGITFDLSNHMVIIGLFIGGLIPFLFGAMAMEAVGRAAGSVVVE
                     VRRQFKEIPGIMEGTAKPEYSKAVDMLTVAAIKEMMIPSLLPIAVPIIVGLVLGPQAL
                     GGLLVGTIVTGLFVAISMTTGGGAWDNAKKYIEDGNFGGKGSEAHKAAVTGDTVGDPY
                     KDTAGPAINPLIKIMNLVALLIVPLIV"
     gene            3096..>3250
                     /locus_tag="VIS73_03210"
     CDS             3096..>3250
                     /locus_tag="VIS73_03210"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY5762194.1"
                     /translation="MAQPNIVPVVICAGIYVAHNLAGLYGIAIAATAMLSMTGIIVAL
                     DAFGPIT"
BASE COUNT          625 a         1093 c         1006 g          526 t
ORIGIN      
        1 aagctgacct caccgcttcc gccaagccgc aggaaaggcg acttggccgc cagatcgtcg
       61 ccgcgtagca cgccgtcgcg aaccttgaac gacgccgtca gctctgagaa atcggtttgt
      121 gcatcggact ggacggcaac ctcgctcgtg gcgccctgcg agaacttcgc cttcagatcg
      181 cggaacgagc ggccgagatt cacgcccttg agagcgccat tgcgaacagc caactgcaga
      241 tcgccggcca gcgcgcgctt catcgcgcca accgtcgccc cgcgagccgc cagatcggca
      301 acgacgtcgg cacgaccggc caaaagatcg gtcgaggcaa ggtcgcgcag cagcggattc
      361 agatcgacgc cggtgagctt gacgcgggta ttgaccgcat tgccggccgc tacggcgctc
      421 gctgctccgt cgagcgtgcc gccgtagaac gccagctgca gcggcgaaag gtcgagccgc
      481 ccgttggcca gccgcgcttc gatcagggcc ttgtcgatga cgatgccgcg cgccttgagt
      541 cctgccgatt cgatcctggc gcgaaccttc ttgccagcca gcgggctcag atcaaccggc
      601 ggatcaaggt ccgccttgcc cactgcattg gctggcggcg ccgacgcctc ggcaggcggc
      661 ggcagcagat aggcatcgaa atccagcttg ccgagttgcg cgtcggccac cagatcaagc
      721 ggcgacagcg cgttcagcgt cgttcgcacg cggcttttcg attcatcgag cgaaaactgc
      781 agatcgactt tgccacgcgg cccatttaga tcaaggtcgg cagagaactc ggtcgcgagc
      841 ttcagcggct tctgcagaag tgtcgaatcg gtcagccccg cgtcaccctt ccagccgctc
      901 agtcgcagca gcatgccggg gttcatctca agccctccgg ccagcgcagc atcggccttc
      961 catcctggcc cgcggaccgc ctgcgtggtc agcacgagct gcggaatctg taacacgcca
     1021 tcggcagccc ccgcgccgcg ccagctcaga ctaggcgaac ggaccgtcgc cgcggtcgtc
     1081 aggccggcca cctcgcccga gaagtcaagc agcagatccc tgagctcggc gatcggccca
     1141 ttgaaaacgt accggacact cgccgcgccg agcttcagct gcagttgcgc aatgccgccc
     1201 gcgcgaccgc cgacagttcc cgacagcgcc tcgaaagcca gctcctgatc acccattcgg
     1261 tagcgcgatg ccagctcgac acgcaggact tcctcagacg cgcctgcttc catgcgcaac
     1321 tcggcgccga cgcgcagttc gccgctggca ccgctgccga gtcgtccggt gacgatctcg
     1381 aagtcgcgca aggcaaactt ctgcttcgcc acccggtcgt gccaggaaag ctcccgaccg
     1441 tcgatgtgga tacccccgat gtcaaagcga atgggcggct tcgccgactc ctctacaggc
     1501 gccgccggcg ctgcgccggc ggtgcccggc gcgccgacga ggtcggcgac gttgagagtg
     1561 ccgtcttcgc cacggaccag tcgggcgcgc aagcccgcca ccgacacctt gtcagcaacc
     1621 agttcaccgg caaacaacgg caggaccgcg accgccagat tggcgctgtc aaagccggcg
     1681 aattccgccg aacccagttt ctccgacagt gaaaccgcac cgagccgtac gccaaggcgc
     1741 ggccagatgg cgagttcggg tacgccggct atctgcaggg tccgcccagt cttctcggcg
     1801 atgctttcct caagcacggc cttgacgcgt tcgccgtcga agagcagcac ggcggcgacg
     1861 atcaccgcca cgatcagcag caacaacccg acgagcagca caagaatggc cttgatccag
     1921 cgtttcatgt ttgcgatttc tccggcgaca ggcgcaacga caatgttgtc tattctagaa
     1981 cccgccccgc ccaggcgaga gcggggcaac aaaaaaggcg gccgaggccg ccttttttgc
     2041 gtgctgcgtg agatcagacg atcagcggca cgatcagcag ggccaccagg ttcatgatct
     2101 tgatcagcgg gttgatcgcc gggccggccg tatccttgta cggatcgcca acggtgtcgc
     2161 cggtcacggc cgccttgtgg gcttcagatc ccttgccgcc gaagttaccg tcttcgatgt
     2221 acttcttggc gttgtcccag gcgcctccgc cggtggtcat cgaaatggcc acgaacaggc
     2281 cggtgacgat ggtaccgacg agcaagccgc cgagcgcctg cggcccgagt acgagaccaa
     2341 cgatgatcgg cacggcgatg ggcagcagcg acggaatcat catttccttg atggcggcaa
     2401 cggtcagcat gtcgacagcc ttcgagtact cgggcttggc ggtgccttcc atgatgccgg
     2461 ggatttcctt gaactggcgg cgcacttcaa cgaccaccga gccagccgca cgaccaacgg
     2521 cttccatggc catggcgccg aagaggaagg gaatcaggcc gccaatgaag aggccgatga
     2581 tgaccatgtg gttcgacaga tcgaaggtaa taccggtagc gccagcagcc tcgagaccat
     2641 gggtgtagtc agcaaacagc accagcgcgg caaggccggc ggaaccgatc gcgtaaccct
     2701 tggtgacggc cttggtggtg ttaccaacgg catccagcgg atcggtgacg ttacgcactt
     2761 cggcgggcag ctcggccatt tcggcaatac caccggcgtt gtcggtgatc gggccgtagg
     2821 cgtcgagggc cacgacgata ccggccatcg acagcatcga ggtcgcggca atcgccaggc
     2881 catacagacc gccgagttcg aatgccgcat agatcgaggc acagaccgac agcacgggca
     2941 gcgcggtggc cttcatcgag atgccgatac cggcaatgat gttggtcgcg tggccagtct
     3001 ggcaggcagc ggcaacgtgc ttcaccggcg cgtactcggt accggtgtag tactcggtaa
     3061 tccagaccat cgccgcggtc agcagcaggc cgataatggc gcagccgaac atcgtgccgg
     3121 tggtgatctg cgcaggcatt tatgtagccc acaacctggc aggcctgtac ggcatcgcga
     3181 tcgccgcgac ggccatgctg tccatgaccg gcatcatcgt ggcgctggac gccttcggcc
     3241 cgatcaccga
//
LOCUS       DATDAA010000095         6641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_496207,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000095 DATDAA010000000
VERSION     DATDAA010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496207"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VIS73_03215"
     CDS             complement(<1..745)
                     /locus_tag="VIS73_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762195.1"
                     /translation="MNVTVAAVATSPVPGAPCAGVCGGDTAGGVDFAAVLLSLSTGSA
                     TAASPETLLGALVDGLPGEPVADDTAAGEAATDPLQNLLAGLLAIPAASAAATAGEGE
                     PLGEAGGDEIALSATNSKRNTAEAQTLLAAKADAAGKLADEAVAANPVLPAAAKLADG
                     KLATVAEPAGADLKTAANDDGSPSAPTQQTLAAPTTNNAARGAAAIAHVQAPVGSPAW
                     AGDMSQRVVMLARSDMQSAQITLNPAHLGP"
     gene            complement(850..1293)
                     /gene="fliJ"
                     /locus_tag="VIS73_03220"
     CDS             complement(850..1293)
                     /gene="fliJ"
                     /locus_tag="VIS73_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEY5762196.1"
                     /translation="MSRPFPLQTVLDLMQNRTDEAARELGRRLSAEQDARKQLNLLEE
                     YRNEYARRFQSASQQGISPTQWSNYRDFLGRLDDAIAQQTRTVERSVTHTVAGQEAFI
                     EQRGKLKAFDTLAQRHERVERRRSDRAEQKQTDENAAHKDQSRQE"
     gene            complement(1311..2681)
                     /gene="fliI"
                     /locus_tag="VIS73_03225"
     CDS             complement(1311..2681)
                     /gene="fliI"
                     /locus_tag="VIS73_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HEY5762197.1"
                     /translation="MRECRQRAEQSQPLRVAGHLIRINGLVMEAAGLRLPLGASCRVL
                     PPQGHGIEAEVVGFNGERLYLMPSDDLYGLSPGARVIPFDNPPHLPRYGEAAAPPAPP
                     PIERARMMPVGTRLLGRVVDGAGRPLDGHGPITTNDSGPLQTRPINPMHRAPITQSLD
                     VGIRAINAVMTVGRGQRMGLFAGSGVGKSVLLGMMARYTAADVIVVGLIGERGREVKE
                     FIEQILGPEGLRRAVVVAAPADTSPLMRLQGAAYATAIAEHFRDEGKQVLLIMDSLTR
                     YAMAQREIALAIGEPPVTRGYPPSVFAKLPQLVERAGNGAAGSGSITAFYTVLTEADD
                     PQDPVADAARAILDGHIVLSRTLADQGHYPAIDIEQSISRAMVSLITPEHLERIRRLK
                     QLYSRFQRSRDLIAVGAYAAGSDPLLDQAIAQMPRIEQFLQQDLSERADTAHSLAALE
                     QLLGAA"
     gene            complement(2708..3397)
                     /locus_tag="VIS73_03230"
     CDS             complement(2708..3397)
                     /locus_tag="VIS73_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEY5762198.1"
                     /translation="MDDVIPKEKLTAYERWELAAFDETQQKQATTAPSEAKPAAATTQ
                     AAPAPVTASDTEALAAATEEARSRGFAEGHEAGYAEGMKAARANAERMARLADAYDAA
                     LKQTEAGLAQDILDLSLAVARQIIRTNLAVRPEILLAVISEAMAMVPAHHGHPTLVLH
                     PSDAAMLHEALGQQIAHTGWRVVEDGALQRGDLRVESTNAELDATLQSRWTAVVETIG
                     TRVDWIGEGDS"
     gene            complement(3415..4416)
                     /gene="fliG"
                     /locus_tag="VIS73_03235"
     CDS             complement(3415..4416)
                     /gene="fliG"
                     /locus_tag="VIS73_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEY5762199.1"
                     /translation="MPPSDDGVEKSAVLLISLGENQAAEVLKHLGPREVQKLGHAMST
                     LKTVPRAKVEGVLDEFHKIVTERAAVHVDTDNYLRSVLTKALGDDKASNLLARILHGG
                     ETSGIEGLKWMDAPTVADLVKNEHPQIIATILVHLERDHASEILGHFTERLRNDVILR
                     IATLEGIQPAALRELNDVMMKLLSGSTSVKKAAMGGVRSVAEILNFMGTANESSVVDA
                     IREYDPDLAQKVLDEMFVFENLLDLDDRSIQMLLREIQSDSLILALKGSSEGLREKIF
                     KNMSQRAAEMLREDLEAKGPVRVSEVEAEQKEILKIVRRLADEGQIVLGGKGDDQFI"
     gene            complement(4406..6148)
                     /gene="fliF"
                     /locus_tag="VIS73_03240"
     CDS             complement(4406..6148)
                     /gene="fliF"
                     /locus_tag="VIS73_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEY5762200.1"
                     /translation="MAAAPPAPATANPLDQARLALGRLSNQQKVALIVSLAAAITVLV
                     GGLMWARAGDYRVLFSNVAERDGGAIITALEQLNVPYRFSDGSGAILVPSDRVHEARL
                     RLASQGLPRGGSVGFEIMESQKFGISQFAEQVNYQRGLEGELARTIQSIGAVQLARVH
                     LAIPKPSVFVRQEQKPTASVFIQLYAGRNLDGAQIAGITHLVASSVPQLPPGNVSIVD
                     QGGNLLSQVRSRGEAGLDPAQIGYLREIESVVAQRIESILAPLAGPGNARVQVAADVD
                     FSQTEQTAEIFRPNNDPQTTAIRSQQSSESANVSPSAQGVPGALTNQPPAPATAPLTS
                     PPVAAANGQRPTPIAGQIAAAGVNAPINSVGQPLNTRKDSTVNYEVDKTIRHTKTAQG
                     AIKRLSVAVVVNHRREVGRDGKAVSKPLGEPEMKQINDLVREAMGFSSERGDTLSVVN
                     AAFSQVEVDLAETPLWKDPDNLALGKDLLRYLVIGAIVAYLLLGVARPLLKVLFAGGP
                     PRAAVGVGTAGTGAMAYGARGQPITIVDQPDEALNVALATYEHKLDKARQVAGENPQV
                     VANIVKDWINPNAS"
     gene            6284..>6641
                     /locus_tag="VIS73_03245"
     CDS             6284..>6641
                     /locus_tag="VIS73_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY5762201.1"
                     /translation="MAHSTAVPDPLESLGKGQVAGPEQALALKQAFEFFNRASADLTQ
                     AYEALQGRVESLTAELAVANGELRRQYQEKEALSDRLAALLSALPVGVVVVDEAGCVG
                     DVNPAAAAMFGEALGGR"
BASE COUNT          935 a         2120 c         2381 g         1205 t
ORIGIN      
        1 gcggacccag gtgcgccggg ttgagcgtga tctgcgccga ctgcatgtcg ctgcgcgcca
       61 gcatcaccac gcgctggctc atgtcgccgg cccaggccgg gctgccgacc ggcgcctgga
      121 catgggcaat tgccgcggcg ccgcgcgctg cgttgttcgt cgtgggcgcg gcaagggtct
      181 gctgcgtcgg tgcactcggg ctgccgtcgt cgttcgcggc ggtcttcagg tccgcacccg
      241 cgggttcggc aacggtcgcc aatttgccat cggcaagttt tgccgctgcc ggcagcaccg
      301 ggttggccgc cacggcttcg tcggccagtt tgccggccgc atcggccttg gccgccagca
      361 gcgtctgcgc ctcggcggtg ttgcgcttgc tgttcgtcgc cgacaaagcg atttcgtcgc
      421 caccggcttc gccgagcggc tcgccttcgc cggcagtggc ggcggcgctg gcggcgggaa
      481 tcgccagcag gccggccagt aggttctgca gcgggtctgt tgcggcctcg ccagctgccg
      541 tgtcgtcggc caccggctcg ccgggcaggc catcgaccag cgcgccgagc agggtttcgg
      601 gtgaggcggc ggtcgccgag ccggtcgaaa gcgacagcag cacggcggcg aaatcgacgc
      661 cgcccgccgt gtcgccgccg cagacgccgg cacaaggcgc gcccggcacc gggctggtgg
      721 ctactgctgc gacagtgaca ttcatgaaga ctcccggaaa attcgggaga aaggtacgca
      781 attgccgtgc cactgtcgtg gcgccattcc cgacggagtc gggccaagcc aaggcaggcc
      841 gaatccgggc tattcctgcc gggactgatc cttgtgcgcg gcgttttcgt cggtctgctt
      901 ctgttctgcg cggtcgctgc gtcgccgttc gacgcgctcg tggcgctgcg ccagcgtgtc
      961 gaaggccttg agcttgccgc gctgctcaat gaaggcttcc tgcccggcta cggtgtgcgt
     1021 gaccgagcgc tcgacggtcc gggtctgctg ggcgatagca tcgtcaagcc ggccgaggaa
     1081 gtcgcggtag ttggaccact gcgtcggtga tattccctgc tgcgaggccg actggaagcg
     1141 ccgcgcgtac tcgttgcggt actcttcgag caaattgagc tgcttgcgcg cgtcctgctc
     1201 ggccgagagg cgccggccga gctctcgcgc cgcctcgtcg gtgcggttct gcatcagatc
     1261 gagaacggtc tgcagtggga agggtcggct catcggcgcg ccagtccggc tcaggcggcg
     1321 ccgagcagct gctcgagggc ggcgagactg tgcgcggtat cggcgcgctc cgagagatcc
     1381 tgctgcagga actgctcgat gcgcggcatt tgcgcgatcg cctggtcgag cagagggtcg
     1441 gagccggccg cgtaggcgcc gaccgcaatc agatcgcgcg aacgctggaa gcgcgagtag
     1501 agctgcttga gccgtcggat tctttcgaga tgctcgggcg tgatcagcga caccatcgcc
     1561 cggctgatcg actgctcaat gtcgatggcc gggtagtgtc cctggtcggc gagcgtgcgc
     1621 gagagcacga tgtggccgtc gaggatcgcc cgcgccgcgt cggcaaccgg gtcctgcggg
     1681 tcgtcggctt cggtcagcac ggtgtagaag gcggtgatcg agccgctgcc ggccgcgcca
     1741 ttgccggcgc gctcgaccag ctgcggcagc ttggcgaaga ccgatggcgg gtagccgcgc
     1801 gttaccggcg gctcgccgat cgccagcgcg atctcgcgct gggccatcgc gtagcgggtc
     1861 agcgagtcca tgatcagaag gacctgcttg ccttcgtcgc ggaaatgctc ggctatcgcc
     1921 gttgcgtagg cggcgccctg cagacgcatc agcgggctgg tgtcggccgg cgcggctacc
     1981 acgactgcgc ggcgcagacc ctcggggccg aggatctgct ctatgaactc tttgacctcg
     2041 cggccgcgct cgccgatcag gccgacgacg atgacgtcgg cggcggtata gcgcgccatc
     2101 atccccagca ggaccgactt gccgacgccc gagccggcga agagacccat gcgctggcca
     2161 cggccgacgg tcatcacggc gttgatggcg cggatgccga cgtcgagcga ctgggtgatc
     2221 ggcgcccggt gcatcggatt gatcggccgc gtctgcagcg gcccgctgtc gttggtggtg
     2281 attggtccgt ggccgtcgag cgggcggccg gcgccgtcga cgacgcggcc gagcaggcgc
     2341 gtgccgaccg gcatcatgcg cgcccgttcg atcggcggcg gcgcaggtgg tgctgcggcc
     2401 tcaccgtagc gcggcagatg cggtgggttg tcgaacggga tgactcgggc gcccggcgac
     2461 aggccgtaga ggtcatcgga gggcatcagg tagagtcgct cgccgttgaa gccgaccact
     2521 tcggcctcga tgccgtgtcc ctgcggtggc agcacgcggc agctggcgcc gagcggcagg
     2581 cgcaggccgg cggcctccat gaccaggccg ttgatgcgga tcaggtggcc ggcgacccgg
     2641 agcggctggc tctgctcggc gcgctgccgg cactcgcgca ggaaggactg gaggcggtcc
     2701 agtgccgtca cgaatcgcct tcgccgatcc agtcgacgcg ggtgccgatg gtttcgacga
     2761 cggcggtcca gcgtgattgg agcgtggcgt cgagttcggc gttggtcgat tcgacgcgca
     2821 ggtctccacg ctgtagcgcg ccgtcctcga cgacgcgcca gccggtatgg gcgatctgct
     2881 gaccaagcgc ctcgtgcagc attgccgcat cggaggggtg gagaacgagc gtcgggtggc
     2941 cgtggtgcgc aggcaccatc gccatcgcct cgctaatcac cgcgagcagg atttcgggtc
     3001 ggaccgcgag gttggtgcgg atgatctgcc gcgccaccgc gagcgacagg tcgaggatgt
     3061 cctgcgccag ccctgcttcg gtctgcttga gtgccgcgtc gtaagcgtcg gcaaggcgag
     3121 ccatgcgctc ggcgttggcg cgcgccgcct tcatcccttc ggcgtagccg gcttcgtggc
     3181 cctcggcaaa gccgcggctg cgggcttctt cggtggcagc ggcgagcgct tcggtgtcgc
     3241 tcgcggtcac cggcgccggc gcagcctgcg ttgtcgcagc tgccggcttt gcctcagatg
     3301 gggcagtggt ggcctgcttc tgctgcgttt cgtcgaaggc cgcgagttcc cagcgctcgt
     3361 aggcggtcag cttttccttg ggaatcacgt cgtccatcga tgagcttccg gcgatcagat
     3421 gaactggtcg tcgcccttgc cgccgagaac gatctggccc tcatcggcga ggcggcggac
     3481 gatcttgagg atttccttct gctcggcctc gacttcggag acgcgaaccg ggccctttgc
     3541 ctcgaggtct tcgcgcagca tttcggcggc gcgctgcgac atgttcttga agattttctc
     3601 gcgcaggcct tccgaggacc ccttgagcgc caggatcagc gagtcggact ggatctcgcg
     3661 cagtagcatc tggatgctgc ggtcgtcgag atcgagcagg ttctcgaaca cgaacatctc
     3721 gtcgagcacc ttctgcgcca ggtcggggtc gtactcgcgg atcgcgtcga cgaccgagga
     3781 ttcgttggcg gtgcccatga agttgaggat ctcggcgacc gagcggacgc cgcccatcgc
     3841 cgccttcttg acgctggtcg atcccgagag cagcttcatc atcacgtcgt tgagttcgcg
     3901 cagcgccgcc ggctggatgc cttcgagcgt ggcgatgcgc aggatcacgt cgttgcgcag
     3961 gcgctcggtg aagtggccga ggatctcgct ggcgtggtcg cgttcgaggt ggacgaggat
     4021 ggtcgcgatg atctgcgggt gttcgttctt gaccaggtcg gcgacggtcg gcgcgtccat
     4081 ccacttgagg ccttcgatac cgctggtctc gccgccgtgg agtatgcgcg caagcaggtt
     4141 gcttgccttg tcatcgccga gtgccttagt cagaaccgag cgcaggtagt tgtcggtatc
     4201 gacgtgcacg gcagcacgtt ccgtgacgat cttatggaac tcatcgagca caccctcgac
     4261 cttagcgcgt ggcaccgttt tcagggtcga cattgcgtgg ccaagtttct gtacctcacg
     4321 tgggccgaga tgcttaagca cttcagcagc ctgattctcg cccagcgaga tcagaagcac
     4381 cgcgctcttc tcaacgccgt cgtcgctagg aggcattggg gttgatccag tccttgacga
     4441 tgttggcgac tacctgcggg ttctcgccgg cgacttggcg tgctttgtcg agcttgtgtt
     4501 cgtaggtcgc cagcgcgacg ttgagtgcct cgtcgggctg gtcgacgatg gtgatcggct
     4561 gtccccgcgc accgtaggcc atagcgccgg tacccgccgt accaacgcca acggccgcgc
     4621 gcggcgggcc gccggcgaag agcaccttga gcagtggccg ggcgacgccg agcagcaggt
     4681 aggcgacgat cgcgccgatg accagatagc gcagcaggtc cttgcccagc gcgaggttgt
     4741 ccgggtcctt ccacaagggc gtctcggcga gatcgacctc gacttgactg aacgcggcat
     4801 tgaccaccga cagtgtgtcg ccacgttcgc tcgagaagcc catcgcctcg cggacgaggt
     4861 cgttgatctg cttcatctcg ggttcgccga gcggcttgct gacggccttg ccgtcgcggc
     4921 cgacctcgcg ccggtggttg acgacgacgg cgaccgagag ccgcttgatc gcgccctgcg
     4981 cggtcttggt gtggcggatg gtcttgtcga cttcgtaatt gaccgtcgag tccttgcgcg
     5041 tgttgagcgg ctggccgacg ctattgatcg gcgcattgac gccggcagcg gcgatctgcc
     5101 cggcgatcgg cgtcgggcgc tggccgttgg cggcagccac cggcggcgag gtcagcggcg
     5161 cggtagccgg ggccggcggc tggttggtca gcgcgcccgg caccccctgc gccgatgggg
     5221 agacgttggc cgactcgctg ctctgctggc tgcggatggc ggtggtttgc gggtcgttgt
     5281 tggggcggaa gatctcggcc gtctgctcgg tctgcgagaa gtcgacgtcg gcggcgacct
     5341 gcacccgcgc attccctggg ccagccagcg gtgcgaggat gctctcgatg cgctgggcga
     5401 cgaccgactc gatctcacgc aggtagccga tctgcgccgg gtcgagcccg gcttcgccgc
     5461 ggctgcgcac ctgcgagagc aggttgccgc cctggtcgac gatggagacg ttgccgggcg
     5521 gcagctgtgg cacgctcgag gccaccagat gcgtgatgcc ggcaatctgc gcgccatcaa
     5581 gattgcgtcc ggcataaagc tggatgaaaa ccgaggcggt cggcttctgc tcctggcgga
     5641 cgaagacgct gggcttggga atcgctagat gtacgcgcgc cagctgtacc gcgccgattg
     5701 actgaatcgt gcgcgccagc tcgccctcga ggccgcgctg gtagttgacc tgctcggcga
     5761 actggctgat accgaacttc tggctctcca taatttcgaa gccgacactg ccaccgcgcg
     5821 ggagaccctg cgaggccagt ctgagccttg cctcgtgcac gcggtcggag ggcacgagga
     5881 tcgcgccgct gccgtcgctg aagcggtagg ggacgttgag ctgctcgagt gcggtgatga
     5941 tcgcgccacc gtcgcgttcg gcgacattgc tgaagagcac gcggtagtca ccggcacgcg
     6001 cccacatcag gccgccgacc agcacggtga ttgccgccgc cagcgagacg atcagcgcaa
     6061 ccttctgctg attactgagg cgaccgagcg caagccgcgc ctgatcgagc ggattcgccg
     6121 tggccggagc gggcggagcg gcggccattt cctagcgggt gcgcacccgg tgggcgcgtt
     6181 gggtcttcgt tggcatcaat atgtgaaaca atccggcggg tcgccatcga gggctgctgg
     6241 tggcaactat tgtaccgatt aatcgctgct aaattcgtgc cagatggctc attcgaccgc
     6301 cgttcccgac ccgttggaaa gccttggaaa aggccaggtt gccggacccg agcaggcgct
     6361 ggcgctcaag caggccttcg agttcttcaa tcgcgcctcg gccgatctga cgcaggccta
     6421 cgaggcgctg cagggccgcg tcgagtcgct gacggccgaa ctcgcggtcg ccaacggcga
     6481 gttgcggcgc cagtatcagg agaaggaagc gctctccgac cggctcgcgg cgttgctctc
     6541 ggcgctgccg gtcggggtgg tggtggtcga cgaggccggc tgtgtcggcg acgtcaatcc
     6601 ggccgccgcc gcgatgttcg gcgaggcgct cggcggtcgc g
//
LOCUS       DATDAA010000096         3985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_216927,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000096 DATDAA010000000
VERSION     DATDAA010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216927"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /gene="lptD"
                     /locus_tag="VIS73_03250"
     CDS             complement(<1..1025)
                     /gene="lptD"
                     /locus_tag="VIS73_03250"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEY5762202.1"
                     /translation="MNYPFRLTTSAALVLLLIVQPTVAVDELVPRLRLSPAPIGLRAP
                     RDAVLPVFFSAQRVVGDMNSVVANGDVEMRQLGLRIGAERITYNAIEDTLEASGAVEL
                     DTTLARLRGNRLLYRPGQRSGRLEELDYEILPPQQSFPPGRLSQGAHGEAAAVVIDSE
                     NQYQLEDATFSTCRPGDDSWFLRVSDLRLDYDSETGDGYHAAVWFKGVPIFYSPWITF
                     PLNNKRRTGFLPPTIGSTSVSGFELTVPYYLNLAPNMDATLTPRYFSKRGSQLNSEYR
                     YLGQSFSGETRLEYLPDDQLREIDRWGYSVQHRQRLPLGLYASANVNGVSDDNYYRDL
                     SSRASTSA"
     gene            1171..2208
                     /locus_tag="VIS73_03255"
     CDS             1171..2208
                     /locus_tag="VIS73_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY5762203.1"
                     /translation="MTRNDALSDWLAAQLPGRRTTLAPASADASFRRYFRVSAEDGRD
                     FAEIGGGPSLIVMDAPPAHEDCRPFIRVAGLFAEAGLHAPKILASDLAQGFLLLTDLG
                     RQTFLQAMTANSADAVGIDRLFGSAIAALVRWQQASRADVLPPYDEALLRRELGLFPE
                     WFIGRHLGATLDEKQQRDLETVFDRLVEAALAQPRVFVHRDYMPRNLMVSTPNPGILD
                     FQDAVYGPISYDVVSLFRDAFVSWDEERTLDWTIRYWEAARAAGLPVAADFSEFWRNF
                     EWMGLQRHLKVLGIFCRLNYRDGKPDYLADLPRFLKYARDVTGRYAALAPLLAILNTI
                     EERSPQVGYTF"
     gene            2225..2902
                     /locus_tag="VIS73_03260"
     CDS             2225..2902
                     /locus_tag="VIS73_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY5762204.1"
                     /translation="MKVMILAAGRGERMRPLTDISPKPLLPVGEKPLIVWHLERLARA
                     GIEEVVINTAHLGERLVAELGDGNTFGLSIQWSPEPVGALETAGGIRHALPLLGDKPF
                     LVISGDIWCDWDASSLATTSRGRDLAHLVLAPNPDHHADGDFTLADGRVQLDGEGERL
                     TYTGIGLFHPKLFATLADNAPLALRVPLREAIAKGRVRGEAHYGEWEDVGTPERLAAL
                     DFRLRHP"
     gene            3005..>3985
                     /locus_tag="VIS73_03265"
     CDS             3005..>3985
                     /locus_tag="VIS73_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEY5762205.1"
                     /translation="MPPIPSFIDRRHALLARMGSGVAILPTAPERLRNRDSHYPYRFD
                     SYFWYLSAFAEPEAVLVLVAGDSPKSLLFCREKDVDREIWDGYRYGPAAAAERFGFDE
                     AHPIAALDAMLPELLSDQPRLWFCFGADPDWDARIADALNTVRARVRAGQRAPSEIHD
                     LRDLLDPMRLVKDDYEIDCLQRAADIASAAHRRAMAACRPGLHEYAVEAEFLHEFRRH
                     GTVPSYPPIVAGGKNACVLHYNDNDAPLAAGDLLLIDAGCEVEGYASDITRTFPVDGR
                     FAGPAREIYELVLAAQQAAIAAVQPGAAFDAPHQATLAVLLDGLIALGLLQ"
BASE COUNT          637 a         1442 c         1274 g          632 t
ORIGIN      
        1 gccgaggtac tggcgcggct cgacaggtcg cggtagtagt tgtcgtcgga aacgccattg
       61 acgttggcgc tcgcgtacag accgagcggc agccgctgcc ggtgctgcac cgaatacccc
      121 cagcggtcga tctcgcgcag ctggtcgtcg ggcaggtatt cgaggcgggt ctcgcccgag
      181 aagctctgcc cgaggtagcg gtactcggaa ttgagctggc tgccgcgctt ggagaagtag
      241 cgtggcgtca gcgtcgcgtc catgttcggc gccaggttca gatagtaagg gaccgtcagc
      301 tcgaagcccg agacgctggt gctgccgatc gtcggcggga ggaaaccggt acgccgcttg
      361 ttgttgagcg gaaaggtgat ccacggcgaa tagaagatcg gcacgccctt gaaccacacg
      421 gcggcgtggt agccgtcgcc ggtctcactg tcgtagtcga gccggagatc tgagacgcgc
      481 aggaaccagg aatcgtcgcc gggccggcag gtgctgaagg tcgcatcctc gagttggtac
      541 tggttctcgc tgtcgattac cacggccgcc gcctcgccat gcgcgccctg cgacagcctg
      601 ccgggcggga aggattgctg cggcggcaga atctcgtagt cgagttcctc gagccgcccg
      661 ctgcgctgtc ccggccggta aagcagccga ttgccgcgca gccgcgccag ggtggtgtcg
      721 agctcgactg caccgctggc ctcgagcgtg tcttcgatgg cgttgtaggt gatccgctcg
      781 gcgccgatgc gcagacccag ctggcgcatc tccacgtcgc cgttggcaac tacgctgttc
      841 atgtcgccta ccacgcgctg tgccgagaaa aacaccggca gtacggcgtc gcgcggcgcg
      901 cgcaagccga tcggcgccgg cgacaggcgc aggcgcggca ccagctcgtc aacggcgacg
      961 gtcggctgga cgatcaacag gaggacgagc gcagcgctcg tcgtcaggcg gaaggggtaa
     1021 ttcatcggct cgcggggtcg caccgcgacc gctgccgcga atcgccgatg ctagaatccg
     1081 cgaattctaa cagccggccc accggccgcg gcagccgggc aaccggcgcc aggccaccgg
     1141 tgcgacccgg cggcgccccc gacgaaaccg atgacccgca acgacgcact ttccgactgg
     1201 ctggccgcgc aattgccggg gcggcgtaca acgctcgcgc cggcctcggc cgatgccagc
     1261 tttcgccgct atttccgcgt cagtgccgag gatggtcgtg actttgccga aatcgggggc
     1321 ggcccctcgc tgatcgtcat ggacgcgccg cccgcgcacg aagactgccg accgttcatc
     1381 cgggttgccg ggctgttcgc cgaggccggt ctgcacgcgc cgaaaatcct tgccagcgac
     1441 ctcgcccagg gtttcctgct cctcaccgac ctcggccggc agaccttcct gcaggcgatg
     1501 accgccaaca gcgccgacgc cgtcggcatc gaccgcctgt tcggcagcgc catcgcggca
     1561 ctggtgcgct ggcagcaggc gagccgtgcc gatgtcctgc ccccctacga cgaggcgctg
     1621 ctgcgtcgcg agctgggcct cttccccgag tggttcatcg gccgccacct aggcgccacg
     1681 ctcgacgaga agcagcagcg cgacctcgaa accgtattcg accgcctcgt cgaagcggcg
     1741 ctggcgcaac cgcgggtctt cgtccatcgc gactacatgc ctcgcaacct gatggtcagc
     1801 acgccgaatc cgggcattct cgatttccag gacgccgtct acggaccgat cagctacgac
     1861 gtcgtctcgc tgttccgcga tgccttcgtc tcctgggacg aggagcgcac gctcgactgg
     1921 acgatccgct attgggaagc ggcgcgcgcc gccgggctgc cggtcgccgc cgacttctcc
     1981 gaattctggc gcaacttcga atggatgggc ctgcagcgcc acctcaaggt gctcggcatc
     2041 ttctgccgac tcaattaccg cgacggaaaa cccgactacc tcgccgacct gccgcgtttc
     2101 ctcaagtacg cgcgcgacgt aaccggccgt tacgcggcgc ttgcgccgct gctggcgatc
     2161 ctcaatacga tcgaggaaag atcgccgcag gtcgggtaca ccttctgatg gcgagctcag
     2221 tccaatgaag gtgatgatcc tcgccgctgg ccgcggcgaa cgcatgcggc cgctcaccga
     2281 catctcgccc aagccgctct tgccggtcgg cgaaaagccg ctgatcgtct ggcacctcga
     2341 acgcctggcg cgcgccggca tcgaggaggt cgtcatcaat accgcccacc tcggcgagcg
     2401 actcgtcgcc gaactcggcg acggcaacac cttcggactg tcgatccagt ggtcgcccga
     2461 accggtcggc gcgctcgaaa ccgccggcgg catccggcac gcgcttccgc tgctcggcga
     2521 caagccgttt ctggtcatca gcggcgacat ctggtgcgac tgggatgcca gctcgctcgc
     2581 caccacctcg cgcggccgcg acctcgcgca cctcgtgctg gcgcccaatc ccgatcacca
     2641 cgccgacggc gatttcacgc ttgccgacgg ccgcgtccag ctcgacggcg aaggcgaacg
     2701 gctgacctac accggcatcg gactgttcca tcccaaactg ttcgccacgc ttgccgacaa
     2761 cgcgccgctg gcactacgcg tgccgctgcg cgaggcaatc gccaagggtc gcgtgcgcgg
     2821 cgaggcgcat tatggcgaat gggaagacgt cggcaccccc gaacggctgg ccgccctcga
     2881 tttccggctg cgccatccct gagcatcggg gcgccggaac gcgccgcgcc atagcgctgt
     2941 ccattcgcaa ccggccacgc cgaaaccgac agagccgcaa ttgactgagc gacccgcccc
     3001 caccatgccg ccgatcccga gcttcatcga ccgccgccac gcgctgcttg cccgcatggg
     3061 ttcgggcgtc gcgatcctgc cgaccgcacc cgaacgcctg cgcaaccgtg acagccacta
     3121 cccctatcgc ttcgacagct acttctggta cctgagcgcc tttgccgagc ccgaagccgt
     3181 gcttgtgctg gtcgccggcg acagcccgaa gagcctactg ttctgccgcg agaaggacgt
     3241 cgatcgcgag atctgggacg gctatcgcta cggtcccgcc gcagccgccg agcgcttcgg
     3301 cttcgacgaa gcgcacccga ttgcggcact cgacgcgatg ctgcccgagt tgctgagcga
     3361 ccagccgcgc ctgtggttct gcttcggcgc cgaccccgac tgggatgcgc gcatcgccga
     3421 tgcgctcaac accgtgcgcg cccgcgtccg cgccggacag cgcgcgccct cggaaatcca
     3481 cgacctgcgc gacctgctcg acccgatgcg actggtcaag gacgactacg agatcgactg
     3541 cctgcagcgc gccgccgaca tcgcctcggc cgcgcaccgg cgggcgatgg ctgcctgccg
     3601 tcccggcctg catgaatacg ccgtcgaagc cgagttcctg cacgagttcc gccgccacgg
     3661 cacggtgccc tcgtatccgc ctattgttgc cggcggcaag aacgcctgcg tcctccacta
     3721 caacgacaac gacgcaccgc tcgccgccgg cgacctgctg ctgatcgacg ccggttgcga
     3781 ggtcgagggc tacgcctccg acatcacgcg gactttcccg gtcgacggcc gtttcgccgg
     3841 cccggcccgc gaaatctacg agctggtgct cgccgcccag caggcggcga ttgccgccgt
     3901 gcagccgggt gccgccttcg acgcgccgca tcaggcgacg ctggccgtgc tcctcgacgg
     3961 actgatcgcg ctcggcctgc tacag
//
LOCUS       DATDAA010000097         2196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_76883,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000097 DATDAA010000000
VERSION     DATDAA010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76883"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VIS73_03270"
     CDS             <1..827
                     /locus_tag="VIS73_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY5762206.1"
                     /translation="LRYGDLDPLRLALSGLPPTEAARNRLLLARIAAARAEIASDTRT
                     DLQQTEALAAQTRAQRQRLDRVAKQQRERQADLVAQQRQHRQVVKRISRQISEQRRTV
                     DKLRRDEGRLSDLVRKLDQLLSGRAKPVTAPRNLSRPSGGYASYDRNSRFARQRGELK
                     RPAPGRALRRESELKGVFFSVPDGTEVAAVAAGQVVFSDWLRGFGNLLVIDHGENYLS
                     IYGNNEALLAEVGDSVKAGDTVATAGNSGGQSESGLYFELRFRGEAIDPLPWLRGS"
     gene            876..>2196
                     /locus_tag="VIS73_03275"
     CDS             876..>2196
                     /locus_tag="VIS73_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY5762207.1"
                     /translation="MNWKHAGLIGAGFLTGAILSLHLPAIADRDTRAGLPVEDLRTFA
                     EVFNRVKQDYVEPVEDKTLLTSAISGMLSNLDPHSAYLDADAFKELQVGTHGEFGGLG
                     IEVGMEDGFVKVVSPIEDTPAFRAGLKSGDLIVKIDDTAVKGLTLSEAVKRMRGKPNT
                     KIRLTVVRKGEASPFEVTLTREVIKVNSVRSKLIEPGYGFLRVTQFQDNTVESAVKQF
                     QKLEKDGPLKGLVLDLRNDPGGLLHAAVGISAAFLPAEAKIVSTDGRTEDAKREYRAV
                     PTDYMRGDGTDALATLPASAKKVPMVVLVNGGSASASEIVAGALQDHKRAVIMGTQTF
                     GKGSVQTVLPPPGNAAIKLTTARYFTPSGASIQAKGITPDIVAEEPALGDGSPHRIRE
                     ADLARHLEGEDEAKKPEKSKTDKQAPGSKPADDEEDDTPRARFEFGGE"
BASE COUNT          424 a          749 c          708 g          315 t
ORIGIN      
        1 agctgcgcta cggtgacctc gatccgctgc ggctcgcact gtcgggcctg ccgcccaccg
       61 aagctgcgcg caaccgcttg ctgctcgccc gcatcgccgc cgcccgcgcc gagatcgcca
      121 gcgacacgcg caccgacctg cagcagaccg aagccctcgc cgcgcagacg cgggcgcagc
      181 ggcagcggct cgaccgcgtg gcgaagcagc agcgcgaacg acaggccgac ctggtcgcgc
      241 agcagcgcca gcaccggcag gtggtcaagc gcatctcccg gcagatttcc gaacagcggc
      301 gcacggtcga caagctgcgg cgcgacgaag gccgactctc cgatctcgtg cgcaagctag
      361 accagttgct cagcgggcgc gccaaaccgg ttaccgcacc gcgcaacctg agccggccct
      421 cgggcggcta cgcgagctat gaccgcaaca gccgcttcgc ccgccagcgc ggcgagctga
      481 agcgcccggc gcccgggcgg gcgctgcgcc gagaaagcga actcaagggt gtgttctttt
      541 cggttcccga cggcaccgag gtcgccgcgg tagccgccgg tcaagtggtc ttctcggact
      601 ggctgcgcgg cttcggcaac ctgctggtga tcgaccacgg cgagaactac ctgtcgatct
      661 atggcaacaa cgaggccttg ctcgccgaag tcggcgattc ggtcaaggcc ggggatacag
      721 tcgcgacggc gggcaactca ggggggcaga gcgaatcggg tttatacttt gaactccgtt
      781 ttcgcggcga agcaatcgac ccgctgccct ggttgcgggg ctcgtgaatg gcaccgcgca
      841 acgctgcccc aaacccgtga cttgaaggaa gcggcatgaa ctggaaacac gcaggtctca
      901 tcggcgccgg atttctcacc ggtgccatcc tcagcctgca tctgccggcg attgccgacc
      961 gcgatacgcg cgccgggctg ccggtcgagg atctgcggac ctttgccgag gtcttcaacc
     1021 gggtcaaaca agattacgtc gagccggtcg aggacaagac gctgctcacc agcgcgatct
     1081 cggggatgct ctccaacctc gatccccatt cggcctacct cgatgccgat gccttcaagg
     1141 agctgcaggt cggcacgcac ggcgagttcg gcggtcttgg catcgaagtc ggcatggaag
     1201 acggcttcgt caaggtcgtc tcgccgatcg aggacacgcc ggctttccgc gccggtctga
     1261 aatcgggcga tttgatcgtc aagatcgacg acaccgcggt caagggcctg accctcagcg
     1321 aagcggtcaa acgcatgcgc ggcaagccga acaccaagat ccgcctgacc gtcgtccgca
     1381 agggcgaggc cagccccttc gaagtgacgc tgacgcgtga ggtaatcaag gtcaacagcg
     1441 tccgctcgaa gctcattgag ccgggatacg gctttctgcg cgtcacccag ttccaggaca
     1501 acacggtcga atcggcggtc aagcagttcc agaaactcga gaaggacggc ccgctgaagg
     1561 ggctggtgct cgatctgcgc aatgatccgg gcggtctgct gcacgccgct gtcggcatct
     1621 ccgccgcctt cctgccggcc gaagcgaaga tcgtctcgac cgacggccgc accgaggatg
     1681 ccaagcgcga ataccgcgcg gtcccgaccg actacatgcg cggtgacggt accgatgcac
     1741 tcgcaacgct gcccgcctcg gcgaagaagg taccgatggt ggtactggtc aacggcggct
     1801 ctgcctccgc ctcggagatc gtcgccggcg cgctgcagga tcacaagcgc gccgtaatca
     1861 tgggtacgca gaccttcggc aagggctcgg tgcagaccgt gctaccgccg ccgggcaacg
     1921 ccgcgatcaa gctgaccacg gcgcgctact tcacgccctc gggcgcatcg atccaggcca
     1981 agggaataac gcccgacatc gtcgccgagg agccggcact gggagatggc tcgccacacc
     2041 gcatccgcga agccgatctc gcccgccacc tcgaaggcga ggacgaagcc aagaagccgg
     2101 aaaaatccaa gaccgacaag caggcgccgg gcagcaaacc ggccgacgat gaagaagacg
     2161 acacgccgcg ggcgcgcttc gaattcggcg gcgaga
//
LOCUS       DATDAA010000098         2938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_112486,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000098 DATDAA010000000
VERSION     DATDAA010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112486"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VIS73_03280"
     CDS             <1..315
                     /locus_tag="VIS73_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEY5762208.1"
                     /translation="ELVQNPDILKEVAAREKPPFCVGFAAESGNLDTYAEKKRRDKKL
                     PLVVGNLVQDGFGGDSNALVLFDADGRHLLPPGPKVELARALITEIARRFAATTRKTI
                     RR"
     gene            358..807
                     /gene="dut"
                     /locus_tag="VIS73_03285"
     CDS             358..807
                     /gene="dut"
                     /locus_tag="VIS73_03285"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY5762209.1"
                     /translation="MHHIDVKILDPRLKSSPPHYATPGSAGLDLRAAIAAAIHIQPGE
                     THLIPTGIAIHLADPGLAAMILPRSGLGHTHGIVLGNLVGLIDSDYQGEIMVSTWNRG
                     NQSFTLNPLDRLAQLVVVPVLQVGFNVVDEFPVSDRGEGGFGSTGKA"
     gene            884..1813
                     /locus_tag="VIS73_03290"
     CDS             884..1813
                     /locus_tag="VIS73_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY5762210.1"
                     /translation="MKCAKNLVVVLMLLVLAGCATPLPPLPTVPAGPPPEPKIALVLG
                     GGAARGFAHIGVIRVLEQEKIPIHRIVGTSVGSLIGAIYAADQNSFELEWTAFALEKE
                     HLFDFGVFNAFTGMGPVKGDRLEEFVRNKVPVANIENLKVPFAAVATDLNRGTRVVLD
                     RGSVAKAVRASSAIPGVFNPVEHDGKMLVDGGLVDNIPIAVARELGADIVIAVDISEN
                     VANFNINNVVDVVLQSIAIIFKENVGFRKKEADILIAPLVGAVGSFDFTQKKRVMQAG
                     IDAARIAAPAIHLRIDEWKQAQAAKSAVSPRPQ"
     gene            complement(1886..>2938)
                     /locus_tag="VIS73_03295"
     CDS             complement(1886..>2938)
                     /locus_tag="VIS73_03295"
                     /inference="COORDINATES: protein motif:HMM:NF044548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HEY5762211.1"
                     /translation="YTACPTSNLVLAGTRKIEENQFGYGNLTKKHGVIIVHDEVTAVD
                     AGKKQLVLAKGTLSYDRLILSPGIDFITDKIAGYGADTDQLVPHAWKAGAQTVLLRKQ
                     LEAMKDGGVVVISIPTAPYRCPPGPYERICMISEYLKAAKPKSKIIVLDGNPDIAAKG
                     KLFRAAWEKHYAGLIDYRPGQSAMKIDAAAMKISSEVEDIKADVINLIPPQRAGKIAL
                     AAGLADEGGRWCPVNPVNFASTKAENIHVIGDACVASPMPKSGFSANSQAKVCALNMV
                     AEFNDEKPTDFSAANICYSFVTSNEAISVAAVYKVDDEKILAVEGAGGLSPAASKLEA
                     DYGRAWFANIMAEMKG"
BASE COUNT          515 a          909 c          949 g          565 t
ORIGIN      
        1 gaactggtac agaacccgga catcctcaag gaggtcgcgg cgcgcgaaaa gccgccgttc
       61 tgcgttggct ttgccgccga gagcggtaac ctcgatacct acgcggagaa gaagcggcgt
      121 gacaagaagc tgccgctggt cgtcggcaat ctcgttcagg acggcttcgg cggtgatagc
      181 aacgcgctgg tgctgttcga tgccgatggc cgccacctgc tgccgccggg tccgaaggtc
      241 gaattggcgc gggcgttgat caccgagatc gcgcggcgct tcgccgcgac cacccgcaag
      301 accatccggc gttaagcata gacgcaaggc gtttcaacaa tcggagcggt agcacccatg
      361 catcacattg acgtcaagat cctcgacccg cggctgaagt cttcgccgcc gcattacgcg
      421 accccgggct cggccggcct tgacctgcgc gcggcgatcg ccgcggcaat tcacatccag
      481 cccggcgaaa cacatctgat cccaaccggc atcgcgattc atctcgccga tccgggccta
      541 gcggcgatga tcctgccgcg ctcaggattg ggccacacgc atggaatcgt cctcggtaac
      601 ctcgttggcc tgatcgactc cgactatcag ggcgaaatca tggtgtcgac gtggaatcgc
      661 ggcaatcagt ccttcacgct taatccgctc gaccgccttg cccagctcgt cgtcgtcccg
      721 gttctgcagg tcggtttcaa tgtcgtcgac gagtttccgg tgagcgatcg cggcgagggc
      781 gggtttggca gtaccggcaa ggcttgagcc gataccggac cgcagcgacg ccacctggcc
      841 cgcaagcttc agcgctgcct gcgtcgcgcg aggagataac tacatgaagt gcgccaagaa
      901 cctggtcgtt gtcctgatgc tgttggttct ggcaggctgc gcaacgccgc tgccaccgct
      961 gccaacggtc ccggcaggac caccgccgga accgaagatc gcattggtgc tcggcggcgg
     1021 cgcggcgcgc ggcttcgccc acattggcgt gatccgcgtg ctcgaacagg aaaagatacc
     1081 gatccatcgc atcgtcggca ccagcgtcgg cagtctgatc ggggcgattt atgccgccga
     1141 ccagaacagt ttcgaactcg agtggacggc gttcgcgctc gagaaggagc acttgttcga
     1201 tttcggcgtg ttcaacgcct tcactggcat gggcccggtg aagggcgacc gcctcgagga
     1261 gttcgtgcgt aacaaggtgc cggtcgccaa tatcgagaac ctcaaggttc cgttcgcggc
     1321 cgtggcgacc gacctcaatc gcggcacgcg cgtcgtgctc gacaggggtt cggtggcgaa
     1381 ggccgtgcgc gccagcagcg cgattcccgg tgtcttcaat ccggtcgagc acgacggtaa
     1441 gatgctggtc gatggcggcc tggtcgacaa cattcccatc gccgtggccc gcgagctggg
     1501 tgccgacatc gtcatcgccg tggacatcag cgagaacgtt gccaatttca acatcaacaa
     1561 cgtcgtcgac gtcgttctcc agtcgatcgc cattatcttc aaggagaacg tcggtttccg
     1621 gaagaaggaa gccgacatcc tgatcgcgcc cctcgttggt gccgtcggca gcttcgactt
     1681 cacacagaag aaacgggtga tgcaggccgg catcgacgct gcccgcatcg ccgcgccggc
     1741 aatccaccta cggatcgacg agtggaagca ggcgcaggcg gcgaagtcgg ctgtcagccc
     1801 gaggccacaa tgatccgatt ggacacgtgg gccaacgaaa aagcccgccg aagcgggctt
     1861 tttcgttcag cggtggaacg gttgattacc ccttcatttc cgccatgatg ttggcgaacc
     1921 aggccctgcc gtagtcggct tcgagcttcg aggcggctgg cgagaggccg ccggcgcctt
     1981 cgacggcgag gatcttctcg tcatcgacct tgtagaccgc ggcgacgctg atcgcctcgt
     2041 tgctggtgac gaagctgtag cagatgttgg cggccgagaa gtcggtgggc ttctcgtcgt
     2101 tgaactcggc gaccatgttg agcgcacaga ccttggcctg gctgttcgcc gagaaacccg
     2161 acttgggcat cgggctggcg acacaggcgt cgccgatgac gtggatgttc tcggccttgg
     2221 tcgaggcgaa gttcaccggg ttcaccgggc accagcggcc gccctcgtcg gcgaggccag
     2281 cggcgagagc gatcttgccg gcgcgttgcg gcgggatcag gttgatgacg tcggccttga
     2341 tgtcctcgac ctcggaagaa atcttcatcg ccgcggcgtc gatcttcatc gccgactgcc
     2401 ccggtcggta gtcgatcagc cccgcatagt gcttctccca tgcggcacgg aagagcttgc
     2461 ccttcgcggc gatgtcgggg ttgccatcga gaacgatgat cttcgacttc ggcttggctg
     2521 ccttgaggta ttcgctgatc atgcagatgc gctcgtaggg cccgggcgga cagcggtagg
     2581 gcgcggtcgg gatgctgatg acgacgacgc cgccgtcctt cattgcctcg agctgtttcc
     2641 gcagtagcac ggtctgcgcg ccggccttcc aggcgtgcgg cacgagctgg tcggtatcgg
     2701 cgccgtagcc ggcaatcttg tcggtgataa agtcgatgcc cggcgacaga atcagccggt
     2761 cgtagctgag cgtgcccttg gccagcacca gctgcttctt gccggcgtcg acggcagtga
     2821 cttcgtcgtg gacgatgatc acaccgtgtt tcttggtcag gttgccataa ccgaactggt
     2881 tttcctcgat cttgcgggtg ccggcgagta ccaggttgct ggttgggcag gcggtgta
//
LOCUS       DATDAA010000099        11575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_76966,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000099 DATDAA010000000
VERSION     DATDAA010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11575
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76966"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VIS73_03300"
     CDS             <1..1128
                     /locus_tag="VIS73_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5762212.1"
                     /translation="LAEGMARARRLGKTFYLVSNIYPQGAKLKTWLADLAPAVALAPD
                     ALIMADPGLILLARETWPEMPIHLSVQANTVNAASVRFWKQAGIARVILSRELSLEQI
                     AEIRQACPDTELEVFVHGALCVAYSGRCLLSGYFNHRDPNQGACTNSCRWSYGVHPAK
                     TDPAGDVWLIDEQERREGQYMPIEEDEHGTYILNSKDLRAIEHVQRLVEIGVDSLKIE
                     GRTKSPYYVARACQAYRGAIDDALAGRPFDMGRLAGLEDLANRGYTDGFFQRHHDADY
                     QNLLRGHSTSDRSQYVADVSRYDAASGRVELAAKNRFFVGDRVSLILPGGNREVTIDR
                     LENEGGDEIDGVPGGGHRAFANWPESTVGAFVARHLDGAPG"
     gene            complement(1150..3117)
                     /locus_tag="VIS73_03305"
     CDS             complement(1150..3117)
                     /locus_tag="VIS73_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEY5762213.1"
                     /translation="MHALPLVLLLLATSVLGVLLFRRMGLPPVLGYLLVGSLIGPHAL
                     DLFEASDSAETLAEFGVVFLMFSIGLEFSLPRLYAMKRTVFGLGGLQVVLSMLVAGGL
                     VLAFGAGGVAALALGGAIAMSSTAVLVKLLADRGAIDSGHGRKVIGVLLFQDIAVVPL
                     LVVIPALSGSAQEMAGALGLAFIKASVVLLLVLFFGQKLMGKWFDIVARGKSSELFML
                     NVLLIVLALSYITEIAGLSLALGAFVAGMLISETAYRHHVEEDIKPFRDVLLGLFFVT
                     VGMQLEWSIISERWLVVLLLLLALLLLKGLIVGGLARFFGASAGDSLRVGLWLCAGGE
                     FGFVLLSEAKGLKLIPDDLLQVVLAALVLSMLLAPLIVHFSERLVMRFVSSEWLLRSM
                     ELTKVAAQSFGTDRHAIICGFGRNGQYLARFLAQESISYMALDLDPDRVHDAAAAGES
                     VVFGDATRKETLLAAGLKRASVLVITFSSIDAAEKIITTVQELMPELPVVVRTEDDSH
                     LDRLYSAGAAEVVPEALESSLMLASHALMLLGVPARKVLARIRETRQSHYTLMRGLFQ
                     GGVVDDDAALNENAARLHSVVLHPGAYAVGREIGELGLDGVRAEVTAIRRRGIRAMRP
                     SRDTELQAADVVVLLGSPEQLALAEQRLLQG"
     gene            3184..4176
                     /locus_tag="VIS73_03310"
     CDS             3184..4176
                     /locus_tag="VIS73_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY5762214.1"
                     /translation="MNPSPPPKVRADALIAVAKRALRIEASAINALETRIGQSFVDAA
                     TLILACQGRVIVSGVGKSGHIGRKIAATLASTGTPAYFVHAAEANHGDLGMITHDDLL
                     IAISNSGESEELVSIIPIVKRQGGRVIALTGNAASTLAREADVHLDAGVAEEACPLNL
                     APTASTTAALALGDALAVALLDARGFSERDFARSHPGGSLGRRLLTHVADVMRAAADI
                     PSTGTDTLLAEAVVAMSRGGLGLIAVLDAGRIVGIFTDGDLRRAFERGVDVRSTQLVQ
                     VMTANPQCIRPEQLAVEAVELMERLRINALLVSDGEGRLQGALNMHDLFRAKVI"
     gene            4176..4709
                     /locus_tag="VIS73_03315"
     CDS             4176..4709
                     /locus_tag="VIS73_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5762215.1"
                     /translation="MDAIARARSLRLMAFDVDGVLTDGRLYYNDRGEETKAFSTLDGH
                     GMKMLAACGVTLAIITGRRSPTVEHRARNLGIDRLYQGIADKRATLQALLAELGVAPE
                     HAGYIGDDVVDLPVMRHCGFAAAPANAHAFVRQHAHWIAGARGGEGAVRELCDFILGA
                     QGKLDAALQTYLDTDAR"
     gene            4706..5281
                     /gene="lptC"
                     /locus_tag="VIS73_03320"
     CDS             4706..5281
                     /gene="lptC"
                     /locus_tag="VIS73_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEY5762216.1"
                     /translation="MRSWAAGLAFPLTVFLALAALTGWLRFLTDVPTTVSDSRFRHEP
                     DYRIEDFRASQLSATGEAEYTLAATRLLHYPDNDETVVEAPRVTHQRADGLTVQASAE
                     RGSLNHTAEIIRLAGNVRLIKPAGETPETRFATDELTFIPGAGLATGPGPVTADDGRS
                     RIAGVGFSADLNVRSFTLHSAVKGRFTLTDK"
     gene            5323..5904
                     /gene="lptA"
                     /locus_tag="VIS73_03325"
     CDS             5323..5904
                     /gene="lptA"
                     /locus_tag="VIS73_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEY5762217.1"
                     /translation="MSLSVFAVPAAIAERADRDQAVNLEADRITIDDARKVHVFEGNV
                     RLSQGSLRIQAARIVVSQDASGFQRGTAEGTPARFEQKREGRDEVVSGQATRIEYDAH
                     SERVEFFGQAVVRSGSDEIRGEYIGYDAREERYFAEARQPDGATATTGRVRATIQPRR
                     QTAEDSPAPTDPSLSLQPAGTLAPESKDKEKRQ"
     gene            5901..6677
                     /locus_tag="VIS73_03330"
     CDS             5901..6677
                     /locus_tag="VIS73_03330"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY5762218.1"
                     /translation="MSYQYLIVEREARVARITLNRPAQLNALCDALVDELGEVLRGFD
                     ADPELACVVLTGSEKAFAAGADIAAMKDFDYMDAYLGDYITRNWESVRSFRKPIIAAV
                     AGYALGGGCELAMSCDLIVAGKGAKFGQPEIRLGILPGAGGTQRLTHAVGKAKAMDLC
                     LTARIMDAEEAERAGLVARIVEDAQVVEEAMAMAKTIAGYSLPTVLMIKECINRAFET
                     PLAEGLLFERRQFHAAFALADQKEGMAAFVEKRAARFQNR"
     gene            6917..7486
                     /locus_tag="VIS73_03335"
     CDS             6917..7486
                     /locus_tag="VIS73_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY5762219.1"
                     /translation="MSYFATPEQLAATNKTAVESLLTLANSALASAERITALNLNTAR
                     SALEDGVANTKAMMGVKDVQEAIALQTTLAQPSVEKAVAYSRSVYEISAQTQEEVSKV
                     FESQFSVFQKQVAGLLDKVAKSAPAGSDVAVAAVKSAIAASNSAFDSMNKAAKQVAEI
                     TEANVAAATNATVKAVGATATTGSKKKAA"
     gene            7637..8191
                     /locus_tag="VIS73_03340"
     CDS             7637..8191
                     /locus_tag="VIS73_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY5762220.1"
                     /translation="MANKPDFEQLAAAQKANTEVLMAVMKTGFEGVQRIADLNMAATR
                     ELFDSTIASANTLMSAKDINDAARLNQTMAQPNVERMVDYSRRLYELMAQMQKEMTGI
                     IESQYSQFAKNAAGAIDKTKASAPVGGDVFAAAMKSMLDATNQVFDQMTAATKQITDA
                     TEANVKATTTATSKAVGAGSRRKK"
     gene            8358..9149
                     /locus_tag="VIS73_03345"
     CDS             8358..9149
                     /locus_tag="VIS73_03345"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HEY5762221.1"
                     /translation="MAQAPGGNPPRTTQLEDTQGDYSKYLLYSRTEIRFLIRSIIQKG
                     ALITLHFDHGKAFILTALIAISDDGNWIYLDYGGSEEMNQRALQAEKLIAATMLDKVK
                     IQFSLSGLQATQASGRPVFAAHLPETVLRLQRREFFRLPTPIANPWMCRTTLRIDGEA
                     AQDLDINLLDISGGGVGLMMPTTFQGKLPVGTLLPDCHITLPEEGAIHTTLCVRNAFA
                     SSNRAGQPYLRMGCEFVDLPGTALTLIQRYITRVERDRKGREAGN"
     gene            complement(9230..9538)
                     /gene="fliE"
                     /locus_tag="VIS73_03350"
     CDS             complement(9230..9538)
                     /gene="fliE"
                     /locus_tag="VIS73_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEY5762222.1"
                     /translation="MDTNGIDQVLAQLRTTAASAAGKPAAAGEGDFSAALKSAIDQVN
                     QAQQEGAKLSEKFAAGDADTNLHEVMLSLQKANVSFQEMIQVRNRLVSAYQDIMNMQV
                     "
     gene            complement(9598..10980)
                     /locus_tag="VIS73_03355"
                     /pseudo
     CDS             complement(9598..10980)
                     /locus_tag="VIS73_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
     gene            complement(10987..>11575)
                     /locus_tag="VIS73_03360"
     CDS             complement(10987..>11575)
                     /locus_tag="VIS73_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5762223.1"
                     /translation="ARPELDGETRVRFAERVGEQLRRLERLIQDVLLFARGEAIGREV
                     IPLTELINESAQAVEALRASREVGLVLRPAAADVVVVGARKPLVGALVSLLENALQAS
                     SAESTVEIEASLADGRVEIGVSDSGCGIASENLARIFEPFFTTRGQGTGLGLAIALGV
                     ARSHGGTLEAFSSEGQGSRFVLALPAGAPPVANPG"
BASE COUNT         2201 a         3976 c         3595 g         1803 t
ORIGIN      
        1 ctcgcagagg ggatggcgcg ggcacggcgg ctgggcaaga ccttctacct ggtctcaaac
       61 atctacccgc agggcgccaa gctgaagacc tggctcgccg atctggcgcc ggcggtggca
      121 ctggcgcccg acgcgctgat catggccgac ccgggcctca tcctgctggc gcgcgagacc
      181 tggccagaga tgccgatcca cctctcggtg caggccaaca ccgtcaatgc cgccagcgtc
      241 cgcttctgga aacaagccgg catcgcacgg gtgatcctct cgcgcgaact gtcactagag
      301 cagatcgccg aaatccgcca ggcctgcccc gacaccgaac tcgaggtctt cgtccacggc
      361 gcgctctgcg tcgcctactc ggggcgctgc ctgctctcgg gctacttcaa ccaccgcgac
      421 cccaaccagg gcgcctgcac caactcctgc cgctggtcct acggcgtgca cccggccaag
      481 accgatcccg ccggcgatgt ctggctgatc gacgaacagg agcgccgcga aggccagtac
      541 atgccgatcg aggaggacga gcacggcacc tacatcctca attcgaagga cctgcgggcg
      601 atcgagcacg tgcaacgact ggtcgaaatc ggcgtcgact cgctgaagat cgaagggcgg
      661 accaagtcgc cctactacgt cgcccgcgcc tgtcaggcct atcgcggcgc gatcgacgac
      721 gcgcttgccg gccgcccgtt cgacatgggg cgcctcgctg gactcgaaga cctcgccaat
      781 cgcggctaca ccgacggctt tttccagcgc caccacgatg ccgactacca gaacctgcta
      841 cgcgggcatt cgacttcgga ccgcagccag tatgtcgccg atgtcagccg ctacgacgcg
      901 gcgagcggcc gggtcgaact cgccgccaag aaccgcttct tcgtcggcga ccgggtcagc
      961 ctgatcctgc ccgggggcaa tcgggaggtg accatagacc gtctcgaaaa cgagggcggc
     1021 gacgagatcg atggcgtgcc tggcggcggc caccgtgcct tcgcgaactg gccggaatcg
     1081 acggtcggcg ccttcgtcgc tcgccacctc gacggcgcgc caggctgacg attgccgcca
     1141 gcggctggat cagccctgca gcagtcgctg ttcggccagc gccagttgct cgggcgaacc
     1201 cagcagtacc acgacgtcgg cggcctgcag ttcggtatcg cggctcggcc gcatcgcccg
     1261 gatgccgcgc cggcggattg cggtgacctc ggcgcgcacg ccatcgagtc cgagttcacc
     1321 gatttcacgg ccgaccgcgt aggcgcccgg atgcaggacc accgagtgca gccgcgcagc
     1381 gttttcgttg agggcggcgt cgtcgtcaac gacgccgccc tggaacaggc cgcgcatcag
     1441 cgtgtaatga ctctgccgcg tttcgcggat acgtgcgagc accttgcgcg ccggcacacc
     1501 gagcagcatc agcgcgtgcg aggcaagcat caggctcgac tcgagcgcct ccggaaccac
     1561 ctcggcggca ccggcgctgt acaggcggtc gaggtgcgag tcgtcctcgg tgcgcacgac
     1621 aaccggcagc tctggcatca gctcctggac ggtggtgatg atcttctcgg ccgcatcgat
     1681 actggaaaag gtaataacca agacgctggc acgcttgagg ccggcggcga gcagcgtctc
     1741 cttgcgcgtc gcgtcgccga agaccacgct ctcgccggcc gccgccgcgt cgtggacgcg
     1801 gtcggggtcg aggtcgagcg ccatgtagga aatgctctcc tgcgcgagga agcgcgccag
     1861 gtactgaccg ttgcggccga agccgcagat gatcgcgtgc cggtcggtgc cgaagctctg
     1921 cgcggcaacc tttgttagct ccattgaccg caggagccat tcgctcgata cgaaacgcat
     1981 gacgagccgc tcgctgaagt gaacgatcag cggcgccagc agcatcgaca gcaccagcgc
     2041 cgccagaacc acttgcagca ggtcgtcggg aataagtttt agtcccttgg cctcggaaag
     2101 caggacgaag ccaaactcgc cgcctgcaca tagccaaagg ccaacgcgta gcgaatcccc
     2161 cgccgaggcc ccgaaaaacc gggccagccc gccgacgatg agtcctttca gcaacagcag
     2221 cgccagcagc agcagcagaa caaccagcca gcgctcgctg atgatcgacc attcgagctg
     2281 catgccgacg gtgacgaaga acagcccgag caggacgtcg cggaaaggct tgatgtcttc
     2341 ttcaacgtgg tggcggtagg ccgtctcgga aatcagcatg ccggcgacaa aggcaccaag
     2401 cgccagcgac aggccggcga tttcggtgat gtaggagagc gccagcacga tcagcagcac
     2461 gttgagcatg aacagctcgg aggacttgcc gcgcgcgacg atgtcgaacc atttgcccat
     2521 gagcttctgg ccaaagaaca aaaccagcag caggaccacc gaggccttga taaaggcgag
     2581 gccgagcgca ccggccattt cctgcgccga gcccgacagc gccggaataa ccaccagcag
     2641 cggcaccacg gcaatatcct ggaacagaag cacgccgatg accttgcggc cgtgccccga
     2701 atcgattgcc ccgcggtcgg cgagcagctt caccagcact gccgtcgagg acatcgcgat
     2761 cgccccgccg agcgcgagcg ccgcgacgcc accggcgcca aaggccagca ccagaccacc
     2821 ggcgacgagc atcgacagca cgacctgcag accaccaagg ccgaacacgg tccgcttcat
     2881 tgcgtagagg cgcggcagcg aaaattcgag accgatcgag aacatcagga agaccacgcc
     2941 gaactcggcg agcgtctccg ccgaatcgga ggcttcgaac agatcgagcg cgtgcggacc
     3001 gatcaggctg ccgaccagca agtagccgag caccggcggc aggcccatac gccggaagag
     3061 caggacgccg agcaccgatg tggcgagcag cagcaggacg agcggcaatg cgtgcattcg
     3121 cgcgaaatcc agtgttttag gggcttttgc tatactcgcc gacatcttaa gccagcgctc
     3181 gccatgaacc caagcccccc gccgaaagtt cgcgcggatg cgttgatcgc tgtcgcgaaa
     3241 cgggcgctgc gaatcgaggc cagcgcgatc aacgcgctgg aaactcgcat cggccaatcc
     3301 ttcgtcgatg cggcaacgct gatccttgcc tgccagggcc gcgtcatcgt cagcggcgtc
     3361 ggcaaatccg ggcatatcgg ccgcaagatt gccgcaacgc tcgccagcac cggaaccccc
     3421 gcctacttcg tccacgccgc cgaggccaac cacggcgatc tcggcatgat cacgcacgac
     3481 gacctgctga tcgcgatttc gaattccggc gaaagcgaag aactggtttc gatcatcccc
     3541 atcgtcaagc gccagggcgg ccgggtcatc gcgctgaccg gcaacgccgc ctcgacgctg
     3601 gcacgcgaag ccgacgttca cctcgacgcc ggggtcgccg aggaagcctg cccgctcaac
     3661 ctcgccccca ccgccagtac aacggcagca ctggccctcg gcgatgcgct ggcagttgcg
     3721 ctgctcgacg cgcgcggctt cagcgagcgc gactttgccc gctcgcaccc cggcggcagc
     3781 cttgggcggc ggctgctgac gcatgtggcc gacgtcatgc gcgccgctgc cgacatccca
     3841 tcgaccggaa ccgatacgct gctcgccgag gccgtggtgg cgatgtcgcg cggcggcctc
     3901 ggcctgattg ccgtgctgga cgccgggcga atcgtcggca tctttaccga cggcgacctg
     3961 cgccgggcct tcgagcgcgg cgtcgatgtc cgttcgacgc agctcgtgca ggttatgacc
     4021 gccaatccgc agtgcatccg gcccgagcaa ctcgccgtcg aagccgtcga actgatggag
     4081 cggctgcgca tcaacgcact gctggtttct gacggtgaag gtcgcctgca gggtgcgctc
     4141 aacatgcacg acctgttccg cgccaaggtc atctgatgga cgccatcgcc cgagcccgct
     4201 ctcttcgcct gatggccttc gatgtcgacg gggtcctcac cgacggccgc ctctactaca
     4261 acgatcgcgg cgaggagacc aaggccttca gtacgctcga cggccacggc atgaagatgc
     4321 tcgcggcgtg cggcgtgacg ctggccatca tcaccggccg acgctcaccg accgtcgagc
     4381 atcgcgcccg caacctcggc atcgatcgcc tctaccaggg gatagcggac aagcgtgcga
     4441 cgctgcaggc gcttctcgcc gagcttggtg tcgccccgga acacgccggc tacatcggcg
     4501 acgacgtggt cgacctgccc gtgatgcgcc attgcggctt tgccgcagca ccggccaacg
     4561 cccacgcgtt tgttcgccag catgcccact ggatcgccgg cgcccggggc ggcgaagggg
     4621 ccgtgcgtga gctctgcgac ttcattctcg gcgcgcaagg caagctcgat gcggccctgc
     4681 agacctatct ggacaccgac gcgcgatgag aagctgggcc gccggactcg cctttccgct
     4741 gacggtcttt ctggcgctgg cggcgctgac cggctggctg cgctttctga ccgacgtgcc
     4801 aacgacggtg tctgacagcc gctttcgcca cgaacccgac taccgcatcg aggatttccg
     4861 cgccagccag ctctcggcga ccggcgaagc cgaatacacg ctggccgcca cgcgcctact
     4921 gcactatccc gacaacgacg aaacggtcgt tgaagctccg cgggtcacgc accagcgcgc
     4981 cgacggcctg acggtgcagg cgtccgccga gcgcggctcg ctcaatcaca cggccgagat
     5041 catcaggctc gccggcaacg tacgcttgat caagccggcc ggcgaaaccc ccgaaacacg
     5101 ttttgcgacc gacgaactga cctttatccc cggcgcggga ctggccaccg gccccgggcc
     5161 ggtgaccgct gacgacggac gctcgcggat tgccggcgtc ggctttagcg ccgacctcaa
     5221 cgtccgctcc ttcacgctgc acagcgccgt caaggggcgc ttcacgctca ccgacaaatg
     5281 accaagagaa cccccctcag cagcaccttc gccgccattg caatgagctt gtccgttttc
     5341 gcagttccag cggcgattgc cgaacgcgcc gatcgcgacc aggcggtcaa cctcgaggcc
     5401 gaccggatca ccatcgacga cgcgcgcaag gtgcatgtct tcgagggcaa tgtccgcctg
     5461 tcgcagggta gcctgcgcat ccaggcggcc cgcatcgttg tctcgcagga tgcatcgggt
     5521 ttccaacgcg gcacggcaga agggacgccg gcgcgattcg agcagaaacg cgaggggcgc
     5581 gatgaggtcg tatccggcca ggccacgcgg atcgagtacg acgcgcacag cgagcgcgtc
     5641 gagttcttcg ggcaggccgt ggtgcgctcg ggttccgacg agatccgcgg cgagtacatt
     5701 ggttacgacg cacgcgagga acgctacttc gccgaagccc gccagcccga tggtgccacg
     5761 gccaccacgg gtcgggttcg cgcgacgatc cagccgcgcc ggcaaacggc cgaagactcg
     5821 cccgcgccga ccgaccccag cctgtcgctg caaccggcgg gcacgctcgc ccccgaatcg
     5881 aaggacaagg agaaacggca atgagttacc agtacctgat cgtcgaacgc gaggcccgcg
     5941 tcgcccgaat cacgctgaat cgtccggcgc agctcaacgc cctgtgcgat gcgctggtcg
     6001 acgaactggg cgaagtcctg cgcggattcg acgccgaccc cgaactagcc tgcgtggttc
     6061 tcaccggctc cgagaaagcc tttgcagccg gcgcagacat cgcggcgatg aaggatttcg
     6121 attacatgga cgcctatctg ggcgactaca tcacgcgcaa ctgggaatcg gtccgcagtt
     6181 tccgcaagcc gatcatcgcc gccgtcgctg gttacgcgct cggcggtggc tgcgaactcg
     6241 cgatgtcctg cgacctgatc gttgccggca agggcgcgaa gttcgggcag ccggagatcc
     6301 gcctgggaat cctacccggc gccggcggca cgcaaaggct gacgcacgcg gtgggcaagg
     6361 ccaaggcgat ggatctctgc ctgacggcac ggatcatgga cgccgaggag gccgagcgtg
     6421 ccggattggt cgcgcgcatc gtcgaggatg cgcaggtggt cgaggaagcg atggcgatgg
     6481 cgaagaccat cgccggctat tcgctgccga ccgtcctgat gattaaggaa tgcatcaacc
     6541 gcgccttcga gaccccgctc gccgagggcc tgctcttcga gcgccggcag tttcacgcgg
     6601 ctttcgcgct ggccgaccag aaggaaggca tggccgcctt cgttgagaaa cgggccgcca
     6661 ggttccagaa ccgctagccg gcacgcggca ttgcaatatc gccgtggcgc catcagtcga
     6721 gcctgaagct cggcggcgtt tcgtttgcag tacattgttt ttaaatgatt tacaaattat
     6781 tgctgcgccg caccaaaaaa gtgttgacag aggtctccgc atcgctataa ttcgaattac
     6841 tgcggtgcaa caattcgatt gacgggtgtt gagccgctcc aagttcaccc ttcaacctcg
     6901 taccaaggag atttacatgt cctacttcgc tacccccgag caactggccg ccaccaacaa
     6961 gaccgccgtt gagtcgctgc tgacgctggc caacagcgcc ctcgcctccg ccgagcgcat
     7021 caccgccctc aacctgaaca ccgcccgttc cgcgctggaa gacggcgtcg ccaacaccaa
     7081 ggcgatgatg ggcgtcaagg acgttcagga agccatcgcg ctgcagacca cgctggccca
     7141 gccgtcggtt gagaaggccg ttgcctactc gcgcagcgtc tacgagatct cggctcagac
     7201 ccaggaagaa gtttcgaagg tcttcgaaag ccagttctcg gtgttccaga agcaggtcgc
     7261 tggcctgctc gacaaagttg ccaagtcggc cccggccggt tcggacgttg cggttgccgc
     7321 cgtcaagtcg gccatcgctg ccagcaactc ggctttcgac agcatgaaca aggctgccaa
     7381 gcaggttgcc gagatcaccg aagccaacgt tgccgccgcg accaacgcca cggtgaaggc
     7441 cgtcggcgcg accgcgacga ccggcagcaa gaaaaaagcc gcctgatcaa ggcgccttga
     7501 aggatgcgta agcattaggg aatcccggcc tcgcgccggg attttttttc gccggcgcag
     7561 cctatagtga agtggcgaac ccacccgttt attggcaaac ccaccaagtc gcagcaccat
     7621 tcacaggagg aacaccatgg caaacaaacc ggattttgaa caactcgcag cagcccagaa
     7681 ggccaacacc gaagtactga tggccgtcat gaagaccggc tttgagggtg tccagcgcat
     7741 cgccgacctg aacatggccg ccacgcgcga actgttcgac tcgacgatcg ccagcgctaa
     7801 cacgctgatg agcgccaagg acatcaacga cgccgcccgc ctcaaccaga cgatggccca
     7861 gcccaacgtt gagcgcatgg tcgactacag ccgtcgcctc tacgaactga tggcgcagat
     7921 gcagaaggaa atgaccggca tcatcgaatc gcagtacagc cagttcgcca agaacgcggc
     7981 cggcgcgatc gacaagacca aggccagcgc accggtcggt ggcgatgtct ttgctgccgc
     8041 gatgaagtcg atgctcgacg cgaccaatca ggtcttcgac cagatgacgg ctgccaccaa
     8101 gcagatcacc gatgccaccg aagccaacgt aaaggccacc acgacggcga cgtcgaaggc
     8161 cgtcggtgcc ggctcgcgcc gcaagaagta atcggccaca cgccccaaga gcccggctcc
     8221 cgccgggctt ttttatggcc gcttccgatc gcgccccggc gcgcggcaaa atgccgaggc
     8281 aactgggtta cactccgacg ctgttcgaaa cccggtttcc ggcttttccc cgccaacccc
     8341 gacagcgctg atctttcatg gcccaggctc ccggtggcaa cccaccgcgc acaacccaac
     8401 tagaagatac gcagggcgac tacagcaagt acctgctgta ttcgcggacc gaaatccgct
     8461 ttctgatccg ctcgatcatt cagaagggcg ccctgatcac gctgcatttt gatcacggca
     8521 aggctttcat cctgaccgcc ctgatcgcca tctccgacga cggcaactgg atctaccttg
     8581 actacggcgg cagcgaggaa atgaaccagc gcgcgctgca ggccgagaag ctgatcgcag
     8641 cgacgatgct cgacaaagtc aagatccagt tctcgctctc cggccttcag gcgacccagg
     8701 ccagcggccg ccccgtattc gcggcgcacc tgcccgagac cgtgctgcgc ctgcagcgtc
     8761 gcgagttctt ccggctgccg acaccgatcg ccaatccgtg gatgtgccgc acgacgctgc
     8821 gcatcgacgg cgaggcggcg caagatctcg acatcaacct gctcgacatc agcggcggcg
     8881 gggtcggcct gatgatgccg accacttttc agggcaagtt gccggtcggc acgctgctcc
     8941 ccgactgcca catcacgctg cccgaagaag gcgcaatcca cacgacgctg tgcgtacgca
     9001 acgcctttgc ctcgtcgaac cgcgccggcc agccctacct gcggatgggc tgcgagttcg
     9061 ttgacctgcc gggtaccgcg ctgacgctga tccagcgcta catcacgcgc gtcgagcgtg
     9121 accgcaaggg gcgcgaagcc ggcaactgat cggcgcaaat cgagtcgccg ggcaacggac
     9181 atgaaaaaag ggcctcgcgg ccctttcctg ttggcacgag cgaattcgct cagacctgca
     9241 tgttcatgat gtcctgatag gccgacacca gccggttgcg gacctggatc atctcctgga
     9301 aggaaacgtt cgccttctgc agcgagagca tcacctcgtg cagattggta tcggcatcgc
     9361 cagcggcaaa tttttccgac agctttgccc cctcctgctg cgcctgattg acttggtcaa
     9421 tggccgactt gagcgcggcg gagaagtcgc cctcgcccgc cgctgcgggc ttgccagccg
     9481 ccgatgcagc agtcgtgcgc aactgcgcga ggacctgatc gattccgttg gtatccattg
     9541 attactcctt cccccgctac ctagcaaaac ctgcgccaag cgcgcgtgga tgcgtactca
     9601 gtcaccaaga tagccttcct cgcggtattg cgccagcttg tagcgcagcg cccgctcaga
     9661 aatgcccagc ctggccaccg cctctcggcg cgaaccgccg acctcggcaa gcgtcgccag
     9721 aatgtgttcg cgctcgagat catggatgtt tccggaacgc cggccaccgg ctgccggtcg
     9781 ctcagcgggc gggaccaccg cggcggttgt ttcgacggtc gctatcggcg cggacgcgtt
     9841 gccgtcgacc ggtgccagcc cgaggtcggc agcgcggatt tcgccggcgg cgaggatcat
     9901 cgcgcgctgg atgacatttt ccagctcgcg cacatttccg ggccacgcgt gaacgcgcag
     9961 cgcctgctcg gcgtcgctgc ccaggcgcgg ggcggcgatc gccattcgcg cggcgtgctc
    10021 ctgcaataga cggcgggcga tcggcacgat atcggccgga cgctggcgca gcgccggaat
    10081 cggcagcgga aagacgttga gccggtagta gaggtcttcg cgaaacacgc ctttggcgac
    10141 ggccgcggcc atgtcgcggt tggaggtggc gacgacacga atgtcgaggg caatcggctt
    10201 cttgccgccg actcgctcga cttcacgctc ctgcagcacg cgcagcagct tggcctgcag
    10261 tgcggccggc atttcggtga cttcatcgag cagcagtgtg ccgttctggg cctgctcgaa
    10321 cttgccggcc tgcgcctgct gcgcgccggt gaaagcaccc ttttcataac cgaacagcgt
    10381 cgcctcgagc agggtctcgg gaattgcggc gcagttgatc gcgacgaaag gaccgcgcgc
    10441 gcgggccgaa cgccggtgaa cgaagcgcgc cacgacctcc ttgccgacgc cgctctcgcc
    10501 ggtcaacagc accgttgcat cggtctgcgc gacacgcgcg gcgagcgcaa acaggctgcg
    10561 gctggccggg tcctcggcga ccacgccgtc gtcaccgccg ctctccgcca gccggtagcg
    10621 ctcgatgtgg gcgagcagcg cttcgggttc gaagggcttg agcaggaaat cgcaggcacc
    10681 cgagcgcatc gcctcgacgg cccggtcgac ctcggcgtag gcggtcatca acaacaccgg
    10741 cagttgcggc tggacctcgc ggatcgcctt aagcagcgtg atgccgtcca tcggcgccat
    10801 gcgcacgtcg ctgaccacca acgcaaagcg acgacgggcg atctcggcga gtgccgccgc
    10861 ccccgaatcg acggcgacca cttcctgccg cgcgacttct agcgtgtcac gaacggcttc
    10921 gcgcaaatcg ggatcgtctt cgacgaccta tacgggcaaa gggcgattgt cagtactcat
    10981 cggcttttag cctgggttgg cgaccggcgg cgcaccagcc ggcagtgcta gaacaaaacg
    11041 cgatccctgg ccctcggacg aaaatgcttc caaggtgccg ccgtgcgagc gggcgacgcc
    11101 gagggcgatg gctagaccaa gacccgtacc ctgcccgcgc gtcgtgaaaa agggctcaaa
    11161 gatcctcgcc agattttcgc tggcaatgcc gcagccggaa tcgctgacgc cgatctcgac
    11221 gcggccgtcg gcgaggctgg cctcgatttc aaccgtcgac tcggcagacg aggcctgcag
    11281 cgcattctcg agcaggctca ccagcgcgcc gaccagcggc ttgcgcgcgc cgaccaccac
    11341 gacatcggca gcggccgggc gcagcaccag accgacttcg cggctggcgc gcagcgcctc
    11401 gaccgcctgc gcggattcgt tgatcaactc ggtcagcgga atcacctcgc ggccgatcgc
    11461 ctcgccgcgg gcaaacagga ggacgtcctg gatcaggcgt tcaagacgcc gcaactgctc
    11521 gccgacacgc tcggcaaagc ggactcgtgt ctcgccatcg agttcggggc gggcc
//
LOCUS       DATDAA010000100         2715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_42214,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000100 DATDAA010000000
VERSION     DATDAA010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42214"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VIS73_03365"
     CDS             complement(<1..531)
                     /locus_tag="VIS73_03365"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY5762224.1"
                     /translation="MGALLIRRVFRLLLAGLAAGALIAPVSAQVEGRAYDLGKIFDAI
                     KGVATAVEAATMSEEDEIRIGRELAAKTLGAYAALRSDELQRYLNQVGVWVALQSSRP
                     DLPWRFAAVEGQEVNAFAVPGGTVLVTTGMLALVANEAELACVIGHEVGHIARRHHLS
                     LLQKSALIETGSVLAQQ"
     gene            complement(534..848)
                     /locus_tag="VIS73_03370"
     CDS             complement(534..848)
                     /locus_tag="VIS73_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762225.1"
                     /translation="MRRAVFFALAVLALTPAVAVAEGGFAGFFRSLTGLLGNQKDEPS
                     RRPATSTIGIRGMDEGGAIAAEPSAADLKRLDGWAANRIEAEAAARRRGIAARPVVLA
                     ER"
BASE COUNT          524 a          903 c          779 g          509 t
ORIGIN      
        1 ctgctgcgcg agcacgctgc cggtctcgat cagcgcgctc ttctgcaaca gcgacaggtg
       61 atggcggcgg gcgatgtgcc cgacctcgtg gccaatcacg cacgcgagtt ccgcctcgtt
      121 ggcaaccagc gcgagcatgc cggtagtcac cagcaccgtc ccgccgggaa ccgcgaaggc
      181 gttcacttcc tgcccctcga cggcggcaaa gcgccacggc aggtccgggc gcgacgactg
      241 caaggcaacc cagacaccga cctggttcag gtagcgctgc agttcgtcgc tgcgcagcgc
      301 cgcgtaggcg ccgagcgttt tggcggcaag ttcgcgaccg atgcggatct cgtcctcctc
      361 gctcatcgtc gccgcctcga ccgcagtcgc gacccccttt atcgcgtcga atatcttgcc
      421 cagatcgtaa gccctgccct cgacctgcgc gctcaccggc gcgatcaggg cgccggcagc
      481 aaggcccgcg agcagcaacc ggaacaccct gcgaatcagc agcgcgccca tactcatcgc
      541 tcggccagca ccaccgggcg cgcggcaatg ccgcgtcgcc gcgccgccgc ctcggcctcg
      601 atccgattgg ccgcccagcc atcgaggcgc ttgaggtcgg cagcggatgg ctcggccgcg
      661 atcgcgccgc cctcgtccat gccgcggatg ccgatcgtcg aggtcgccgg acgcctcgac
      721 ggctcatcct tctggttgcc cagcagcccc gtcaacgaac ggaagaagcc ggcaaagcct
      781 ccttcggcga ctgcgactgc cggcgtcaat gcaaggaccg ccagcgcgaa gaagacagca
      841 cgacgcatcg atatcccctc gtttcttcag gttcccggcc agtatagtgc gccaaacctg
      901 cacggcgccc gcagcgcgat ccgccgcctg gcgatcaagc ggccggcctt ggaaaatcct
      961 tcaggcagca cctccccgac ggattcctag tctcgcatga gcacaatcgc gctgctgtct
     1021 gagcgacgac gaagtcccgc gtcgccggat cggctgagaa atcgtcgcgg gtgacgccaa
     1081 agcagtagca caactggctg ccgccggaac cttccttcaa accgacgcgt gtgcgcaact
     1141 gcgccgcgtc gatcatcgac ccgtcatcgc caaaataaac caccacgcag gccggttcat
     1201 cgcagaagaa gtaccgacga gcgcccgccc gccaagccca ggcatcgcgg atgtgatgcg
     1261 tgatggttcg cgcagatacc ccggacgact catgcccgca gagcgggcaa ggctgatctt
     1321 tcgagctagc gtcgtcagga cccgatgaag aacaacactc gttcatgctc cgccccacgg
     1381 cttcgtttgc ggtttggatt atccgcgccc gggcattcgg cggaagatga aacaggcgat
     1441 ccgtcgctct cgacagcggc gccatcagga aaagaagccg gcccgagggc cggcttccgg
     1501 ttcaaggtca tgcgttcgcc ccgacagacc ttggcgcttc ttttcgatga ttgtaggaat
     1561 taaagtctgc cggaccgact gcccggaaag ccccgtattt tctaggggtc ggcaattagg
     1621 aaagtttgcc caaagtctgt cggaatggcg attctaggac aatgccatca cacgcttagg
     1681 aatatagccg gtcgagccct gtcgcaaagc ggttttcggc atgttcaagc aatccctgtt
     1741 tccggcgccc tacgacagca gttcggccaa ttgccgacct tggacttctt gctggactcg
     1801 ggcggcaatg cgccggccac ctgccgttga gaggtggtcc atgtcgaacg gcagtttcgc
     1861 gcatcggcga actcacacta tcgaccccgg agagacattc gccgttcaag tgatccaacg
     1921 acaggttttg gagcgaagca gccgcgcgga acgacgcctg cccccgtgct cctcggtcat
     1981 tttggccagt ggccgcggca gcaattaagc cggacagata cttctgagaa gcgatccaga
     2041 gcgacaaaaa aggctagcta aggctggaga ctctatcttg ccctgatcta ttgaagcgtg
     2101 tggtcagtca gcgactcgca agagcagata caccaacaag cccccgcccc ccacaagcca
     2161 tagaactatg aatccaggcc atctcactct attccagact gtatctccta gacggcgttt
     2221 ggatcccgat cctgggattg cgctctggcg tcgatcgatt cccgtgctta taccggattg
     2281 gagtgtggca ttcatggcgt cccccttctc tttgtttgat gctctctgag cgatttcatc
     2341 gttacgaccc gcccgagcac tcttgcttct ttatcctcgc tttcagccta gcccagactg
     2401 aaggcgggtc aaggtaagcc cattctcaca tcgagagtga tttggtcccg gtgtcgttgt
     2461 ttgattaccg tccgctgatg gccgacttct gggggaatgg tgaatgcatg gctgcagttc
     2521 cgtcgagagt ccgctttttc ttccgacccg gggcagcaac cggccagaag ccgccggtcg
     2581 agcgctccct gaagctgtca ctcagaggtc tggtcgtcgc taacagcagc cggaagacga
     2641 aggcgaactg atcgacctgt cccgaccttt aatccctgtc tgactccggg cggcaatgca
     2701 ctgctcatcg accac
//
LOCUS       DATDAA010000101         3348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_321906,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000101 DATDAA010000000
VERSION     DATDAA010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321906"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VIS73_03375"
     CDS             complement(<1..253)
                     /locus_tag="VIS73_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEY5762226.1"
                     /translation="MNDDTARRLLRELFDAAVGAADPLVCVPPALPAPPRGRCVVIGA
                     GKAAAAMAQAVEASWRADPKKLSGVVVTRYGHSVPTKAIR"
     gene            complement(315..1211)
                     /locus_tag="VIS73_03380"
     CDS             complement(315..1211)
                     /locus_tag="VIS73_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY5762227.1"
                     /translation="MSESLSPIAVVERETGIAKDTLRVWERRYGFPNPVRGPAGDRLY
                     PEMQVARLRLIKRLLDSGHRPGKLMKTDPALLEALSSDPGALCRSDPEAPDLSAVVDR
                     VRRHDGPGVRQQLSQLISRQGLQQFVLETLPMLNEEVGEAWSRGELEVFEEHLYTEHM
                     QVQLRQSIASLPPAHGSPRMLLTSVPEEQHALGLLMLEALFTLEDAYCVSLGTQTPLA
                     DIAAAVRAHRIDVVALSFSGAFPFRQIGPLLADLRRRLPPEVELWAGGGGIRRAEAPD
                     GTHFAHTLPDALALLEDWRRRN"
     gene            1431..2012
                     /locus_tag="VIS73_03385"
     CDS             1431..2012
                     /locus_tag="VIS73_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY5762228.1"
                     /translation="MPQLKPMRHAAATTVRALVVAAALIAPTAAFAACPPVLNHSFPT
                     LQGERPTSLCDYAGKVVLVVNTASKCGFTDQYEGLEALQRTRGAEGLVVLGFPANDFG
                     SQEPGSNQEIAEFCRSTYGVQFPMFAKSRVIGRDANPLFASLAATTGQPPQWNFHKYL
                     IDRSGTRVVSFGSRVRPDDPRLMAQLESFLAAR"
     gene            2064..2876
                     /gene="folE2"
                     /locus_tag="VIS73_03390"
     CDS             2064..2876
                     /gene="folE2"
                     /locus_tag="VIS73_03390"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEY5762229.1"
                     /translation="MNAPEKFFLPDIQSLADDRALAIQEVGVRGVRYPVRLEGADGPQ
                     ATVAQFTMTVGLPAETRGTHMSRFVEFLEHPRGAWTLRQLPGLLGEMLGRLDANAGTI
                     EMSFPWFVVKRAPVSGVESLLDHDVVWRATRAPMDVRTHVELEVRVPVTSLCPCSKKI
                     SDYGAHNQRSLLTLRARLRDAEKRPMAVEELIRFAEESASCEVFGLLKRPDEKWVTER
                     AYDNPKFVEDLVRDVALRLQADSRISVWSVTAENFESIHNHSAYAELRGANV"
     gene            2899..>3348
                     /locus_tag="VIS73_03395"
     CDS             2899..>3348
                     /locus_tag="VIS73_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5762230.1"
                     /translation="MNAPFPKHGGRIAVVGSGIAGLSTAWLLSQAGRHAVLFEAESRL
                     GGHSNTVNVTVDGISHPVDTGFLVYNERTYPNLIALFDWLGVPSVETEMSFSVSLSDP
                     DLEWAGSSLATVFGQSKNLVRPAFWRMLTDILRFNRETTEWLARPQRP"
BASE COUNT          591 a         1088 c         1099 g          570 t
ORIGIN      
        1 cgcgaatcgc cttggtcggc acgctgtgcc cgtagcgggt gacgacgacc cccgagagtt
       61 ttttcggatc agcacgccag cttgcttcga cggcctgcgc catcgctgcg gccgccttgc
      121 cggcgccgat gacgacgcag cggccgcgtg gcggcgcagg cagcgccggg ggaacacaaa
      181 ccagggggtc ggcggcgccg acggcggcgt cgaacagttc gcgcagcaag cggcgggcag
      241 tgtcgtcgtt cattggccga ttgtagggcc gccagcggcc cgttgcgtag cccgctgcgg
      301 tggtcttccc gacctcagtt gcgtctgcgc cagtcctcga gcagggccag cgcgtcgggc
      361 agcgtatggg cgaagtgcgt gccatcgggc gcttcggcgc gacggatgcc accgccgccg
      421 gcccacagct cgacctctgg cggcagccgc cggcgcaagt cggcgagcag cgggccgatc
      481 tggcggaacg gaaaggcgcc cgagaacgac agcgcgacga catcgatgcg atgcgcacgt
      541 accgctgcag cgatgtcggc gagcggggtc tgggtgccaa gcgagacaca gtaggcgtct
      601 tccaaggtaa acagggcctc aagcatgagc aggccgagcg cgtgctgctc ctcgggtacc
      661 gaggtcagca gcatgcgcgg gctaccgtgg gccggtggca gcgacgcgat cgactgccgc
      721 agctgaacct gcatgtgctc cgtgtacaag tgctcttcga atacctccag ttcgccgcgg
      781 ctccacgcct cgccgacctc ctcgttcagc atcgggagcg tttccagcac gaactgctgc
      841 agaccctggc ggctgatcag ttgcgacagc tgttggcgga cgccgggtcc gtcgtggcgg
      901 cgcacgcggt cgacgacggc gctgagatcg ggggcttctg gatcgctgcg gcacagcgcg
      961 ccgggatcgg atgagagcgc ctcgagcaag gccggatcgg tcttcatcag cttgcccggg
     1021 cggtggcccg aatcgagcag acgcttgatc agccgcaggc gtgccacctg catctcgggg
     1081 tagagacggt cccctgccgg cccgcggacc gggttgggga agccgtagcg gcgctcccag
     1141 acgcgcagcg tgtccttggc gatgccggtt tcccgctcga cgacagcgat gggcgaaagc
     1201 gattcggaca tgacggcctt tccttgtctt gttcagatat gtctaggaca aaacagattg
     1261 taaaggttcc gcgattctag ctagaaaaaa taaaaaccac gaaaaaacaa cgcagaaaag
     1321 cggatcgtga gccggcaacg aaaaaaattt gaactagaca agaatcctgt ttgtctagga
     1381 cggcaagggt gcattgtttg caccgagccg accaccaagg agccaccaag atgccgcaac
     1441 tcaagccaat gcgccatgcc gccgccacga ccgttcgcgc gctggtcgtc gccgccgcgc
     1501 tgatcgcgcc gacagcggcc tttgccgcat gcccgccggt gctcaaccac agcttcccga
     1561 cccttcaggg cgaacgcccg acctcgctgt gcgactacgc tggcaaggtc gtgctggtgg
     1621 tcaacaccgc gagcaaatgc ggtttcaccg accaatacga ggggctggag gcgctgcagc
     1681 gcacacgcgg cgccgaaggt ctggtcgttc tgggttttcc ggccaacgat ttcggcagtc
     1741 aggagccggg cagtaaccag gagatcgccg agttttgccg cagcacttac ggcgtgcagt
     1801 tcccgatgtt cgccaagagc cgggtgatcg gccgagatgc caatccgctg ttcgcgtcac
     1861 tggcggcgac gaccggccag ccgccgcaat ggaacttcca caagtatctg atcgaccgca
     1921 gcggcacgcg ggtggtgagt ttcggcagcc gcgttcgccc tgatgacccg cgcctgatgg
     1981 cgcagctcga gtcctttctc gcggcgcgct gaagcgccag atcgccacca aacttagatg
     2041 tttccccacg cgaaggagtc accatgaacg ccccggaaaa attctttctc cccgatatcc
     2101 agagtcttgc cgacgatcgt gccctggcga ttcaggaggt cggcgttcgc ggcgtccgct
     2161 atccggtccg cctggaaggg gccgacggcc cgcaggcgac ggtcgcgcag ttcacgatga
     2221 ccgtcggact gccggccgag acgcgcggca cgcatatgtc gcgcttcgtc gagttccttg
     2281 agcatccgcg cggtgcttgg acgttgcgcc agttgcccgg actgctcggc gagatgctcg
     2341 gtcggttgga cgccaacgcc gggacgatag agatgagctt tccttggttt gtcgtcaagc
     2401 gggccccggt ctccggggtc gaaagcctgc tcgaccacga cgtggtctgg cgagcgaccc
     2461 gcgcgccgat ggatgtgcgg acccacgtcg aactcgaagt gcgcgttccg gtgaccagcc
     2521 tctgcccgtg ctcaaagaag atctccgact acggtgcgca caaccagcgc tcgctgctga
     2581 cgctgcgggc gcgcctgcgc gacgccgaga agcggccgat ggcagtcgag gaactgatcc
     2641 gcttcgccga ggaatcggcc tcgtgcgaag tcttcggcct gctcaagcgg cccgacgaga
     2701 agtgggtgac cgagcgcgcc tacgacaacc cgaagttcgt cgaggacctg gtccgcgacg
     2761 tcgcgctgcg cctgcaggcc gactcgcgca tctcggtctg gtcggtcacc gccgagaact
     2821 tcgagtcgat tcataaccat tcggcctacg cagagttgcg tggggccaac gtttgaattt
     2881 aacgaacgga gcatgccgat gaacgccccc tttccgaagc atggcggccg gatcgccgtc
     2941 gtcggttccg gcatcgccgg cctgtcgacg gcctggctgc tatcgcaggc aggacgccac
     3001 gcggtgctgt tcgaagccga gtcgcggctc ggcggccata gcaacaccgt caacgtcact
     3061 gttgacggca tctcgcatcc ggtcgacacc ggcttcctcg tctacaacga gcgtacctac
     3121 cctaatctga tcgcgctgtt cgactggctc ggcgtgccca gcgtcgagac cgagatgtcg
     3181 tttagcgtca gcctcagcga tccggacctc gagtgggccg gatcgagtct ggcgacggtc
     3241 tttggccagt cgaagaatct ggtccggccg gcattctggc gaatgctcac cgacatcctc
     3301 cgtttcaacc gcgaaactac cgagtggttg gcgcgccccc agcgccca
//
LOCUS       DATDAA010000102         7804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_496604,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000102 DATDAA010000000
VERSION     DATDAA010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496604"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(398..1174)
                     /locus_tag="VIS73_03400"
     CDS             complement(398..1174)
                     /locus_tag="VIS73_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY5762231.1"
                     /translation="MTVLRILLVDDEAPARTRLRVLLEDIASQLTTTVVAEAANGVEA
                     LAAWQAEKPDLLLVDIRMPKMDGLELARHLAGEENPPAVIFTTAYEAHALEAFEVAAV
                     DYLLKPVRADRLLAALKKAIARLPGERGAALEGVAAQREDARRYFSAQVMGKLTLIPV
                     EEVLYLRAEQKYVSAVTAGRTHLLDESLTRLEEEFPDRFIRLHRGVLIARDAIAGFQR
                     EREGEDGEGEHWIAVVRGTNEHLPVSRRQWPTVKAFAKRA"
     gene            complement(1177..2247)
                     /locus_tag="VIS73_03405"
     CDS             complement(1177..2247)
                     /locus_tag="VIS73_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY5762232.1"
                     /translation="MDKSIRELPEAPLLPDFRNLGIALRILLGGVVIAAAAALVGAPR
                     IADWPQVFVANAIHVVPVLLGTLGLCVLLTRPLSRLPGYAATGFVVALAVGIAVAVDD
                     TLIYIGLAEPQRGGLLRSALLAAGATMILLTYLALRARAMRPAAAEARFAALSARIRP
                     HFLFNSLNAVLSLIRSDPRRAEAALESVADLFRVLMRDPRKLVPLSEEIALSRQYLDL
                     ERLRLGEDRLRVNWEVNAVPEDALVPPLMLQPLLENAVYHGIEPADDGGELHIAFAHG
                     NDGIVIELDNPISIDRHPDRGGKPAPTPERRRAARGGNHMALANIRERLDLHFDLDAR
                     LETRSENGRYHVRIVLPYRTGR"
     gene            2368..3765
                     /gene="argH"
                     /locus_tag="VIS73_03410"
     CDS             2368..3765
                     /gene="argH"
                     /locus_tag="VIS73_03410"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY5762233.1"
                     /translation="MSHSDAANPAYTWAGRFSEPVSDLVKRYTASVDFDQRMWRQDIA
                     GSLAHAKMLAKQGIIGTDDLQAIEGGMATIVSEIETGQFKWSVDLEDVHFNIEKRLTT
                     LIGDSGKRLHTGRSRNDQVATDIRLWLRDAIDTIVGLLGDYQRALIDLAEQHAATPMP
                     GFTHLQVAQPVTFGHHLLAYVEMAERDRERMRDCRRRTNRLPLGAAALAGTTYPIDRE
                     FVAKELGFEGVCENSLDAVSDRDFAIEFCAAAALVMTHVSRFSEELVIWMSPRVGFVR
                     IADRFCTGSSIMPQKKNPDVPELARGKTGRVNGDLIALLTLMKGQPLAYNKDNQEDKE
                     PLFDAADTVIDTLRIFADMVGGITPQPEAMAAALTQGFATATDLADYLVKKGLPFRDA
                     HEAVGLAVRAAETRGVDLPQLPLEELRRFSPLVGEDVFAVLTVTGSLASRKHFGGTAP
                     EQVRAAIVRARARLG"
     gene            3779..5059
                     /locus_tag="VIS73_03415"
     CDS             3779..5059
                     /locus_tag="VIS73_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5762234.1"
                     /translation="MDRLGKYEIEREIGRGATSVVFLGNDPFAQRQVAIKVGYPDILK
                     DAERGKVYSHLFINEASLVGKLQHPHIVQIFDAVVGEGVAYIVMEYVPGTTLEAFCKP
                     DTLLSFERIVEIIFKCTRALNFACQLGITHRDIKPANILIAQSETGFGDIKISDFGAA
                     ITTSTDRTMVSGIGSPAYMSPQQVREESLDLRTDIYSLGVVMYQLLTGQLPFQASSNY
                     NMVYQIIHTDPTPPERLRDEVPQRLADIVAKAMSKRIESRYQTWEEFAQDLAQAFRAR
                     SNEKSRFADSEKFSTLRALPFFERFSDVEIWEAVRFSQWEHVRPGHAIMKDGEDGDFF
                     CFLAEGEVKVAKNGKTLNVLSAGDCFGEMAVIGQASHVRGADVVALGDAKVIRIPGEA
                     LHAASESCRLQFYQSFLEVLANRLTLANTRLAAF"
     gene            complement(5095..5427)
                     /gene="grxD"
                     /locus_tag="VIS73_03420"
     CDS             complement(5095..5427)
                     /gene="grxD"
                     /locus_tag="VIS73_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEY5762235.1"
                     /translation="MSDIQETIRQQISTHPVVLYMKGTPQFPQCGYSANAAQMIQRCG
                     VKNFFAVNVLPDPALVAGLREFANWPTLPQFWVKGEFVGGHDIMKEMFNAGELQQLLA
                     EFADQKSA"
     gene            complement(5509..6366)
                     /gene="prmC"
                     /locus_tag="VIS73_03425"
     CDS             complement(5509..6366)
                     /gene="prmC"
                     /locus_tag="VIS73_03425"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY5762236.1"
                     /translation="MVAATGTDGTVTVREAVDAVRRRIARLDARLLVQHIVGIGHAEF
                     LAHPERRLSHDEANAFMALVNRRANGEPLAYLTGEREFFGRSFAVTADVLIPRPDTER
                     LVLAAIEVAEERGGEAAILDLGTGSGAIAVTLALELPGARLTATDLSAAALTVAGRNA
                     AVLGATVSFVASDWYAALGPTQFDLIVSNPPYVAAGDPHLAQDGLPFEPDLALTDGGD
                     GLACLREIIAGAPAFLAPGGQLLLEHGYDQAAAVRALLARAGFIAIASRRDLSGHERV
                     SGGRWQPPA"
     gene            complement(6369..7451)
                     /gene="prfA"
                     /locus_tag="VIS73_03430"
     CDS             complement(6369..7451)
                     /gene="prfA"
                     /locus_tag="VIS73_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY5762237.1"
                     /translation="MKPTLRDRLERLAMRLEELDSLLSSPETAANPDALRRLSQERAE
                     VEPVVTLFARFCATEAAIGEARAMLADPEMAEMAAEEIAEREAALPVIDFELQKLLLP
                     KDANDERNVFLEIRAGTGGDESALFAADLFRMYSRFAERQRWRVEIVSASESDLGGYR
                     EVIARVTGTGVYSRLKFESGGHRVQRVPETEAQGRIHTSACTVAVMPEADELAEIDIN
                     PADLRIDTFRASGAGGQHVNKTDSAVRITHLPSGLVVECQDDRSQHRNRAQAMSVLAA
                     RLKDKQTRDAQAKEAAARKSLVGSGDRSERIRTYNFPQGRITDHRINLTLYKIAQIMD
                     GDLDELLGALAAEYQAEQLAALAETV"
     gene            complement(7470..>7804)
                     /locus_tag="VIS73_03435"
     CDS             complement(7470..>7804)
                     /locus_tag="VIS73_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY5762238.1"
                     /translation="SRQAAVVEAEAIITNRVDGFVHWLETRSTTVPTIRSLRDSAERM
                     RRHELDHAMKLIARGEPVEKIVELLSQRLTNKFLHAPTQALNQADPEHRDELQQAAAR
                     LFHLHPGE"
BASE COUNT         1318 a         2559 c         2605 g         1322 t
ORIGIN      
        1 ctgtcaccac cacggggata ctggagtgag ccggaaagcg gcgccaccat ccttccgctg
       61 cgagccgtcc ccggctgtag cagaccggga acggagcgtg gccttgagag ttgagaacgc
      121 gcaagacctg ccccgggttt agtcgctgga tcgcgatcgg cgcgttgtgc tttcaagcca
      181 cagctgatcg gcgcactgcg gtccggcctg gatgccggtg ggcagacgcc cacggcgacg
      241 gtggccgctt tgatcgcgta gccgtaaggc aagtatcaga gcgaatcggt cgctcggacg
      301 gctgctctgt cgctttggcc gacggcggct tatggccgat accggcactt cacgcgcatc
      361 gccaagagag gttcgcgcgc cgagcccccg gtcgttctca cgcccgcttc gcaaaggcct
      421 tcaccgtcgg ccactggcgg cggctgacgg gaaggtgttc gttggtgcca cgaacgacgg
      481 cgatccaatg ttcgccttcg ccgtcctcgc cctctcgttc acgctggaag ccggcgatgg
      541 cgtcgcgggc gatcagtacg ccgcggtgca ggcggataaa gcggtctgga aactcctctt
      601 cgagcctggt cagcgattca tcgagcaggt gcgtgcgccc ggcggtgacg gcggagacgt
      661 acttctgttc ggcgcgcagg tagaggactt cctcaaccgg aatcagcgtc agcttaccca
      721 tcacctgcgc cgaaaagtag cgccgcgcgt cctcgcgctg ggccgcgacg ccttcgagcg
      781 ccgcgccgcg ttcgccgggc aggcgggcaa tcgccttctt cagcgcggcg agcaggcggt
      841 cggcgcgcac cggcttgagc aggtagtcga cggcggcgac ctcgaatgct tcgagcgcgt
      901 gcgcctcgta ggcggtggtg aaaatcaccg ccggcggatt ttcctcgccg gcgaggtggc
      961 gcgcgagttc gaggccgtcc atcttcggca tgcggatgtc gacgagcagc agatcgggct
     1021 tctcggcttg ccaggcggcg agcgcttcga caccgttggc ggcttcggca accaccgtgg
     1081 tcgtcagctg cgaggcgatg tcttcgagca gcacgcgcag gcgtgtgcgg gccggggctt
     1141 catcgtcgac gagcaggatg cgcagcacgg tcatgatcag cgtccagtcc ggtagggcag
     1201 cacgatgcgg acatggtagc ggccgttttc gctgcgagtt tcgagacgcg cgtcgaggtc
     1261 gaaatgcagg tcgagccgct cgcgaatgtt ggccagcgcc atgtggttgc cgccacgcgc
     1321 cgcacgccgg cgctcgggtg tcggtgccgg cttgccgccg cggtcgggat ggcgatcgat
     1381 cgaaatcgga ttgtcgagtt cgatgacaat gccgtcgttg ccgtgggcga aagcgatgtg
     1441 cagttcacct ccgtcgtcgg caggttcgat gccgtggtaa acggcgtttt cgagtaaggg
     1501 ttgcagcatc agcggcggca ccaaagcgtc ttcgggcacg gcgttgactt cccaattgac
     1561 tcgcaaccgg tcctcgccca gccgcaggcg ttcgaggtct aaatactgcc ggctcagggc
     1621 gatctcctcg gagagcggca ccagttttcg cgggtcgcgc atgagcacgc gaaaaaggtc
     1681 tgcgaccgat tcgagcgccg cctcggcacg gcgcggatcg ctgcggatta gcgagagcac
     1741 agcgttgagg ctgttgaaca ggaagtgtgg gcgaatgcgc gccgagagcg cggcgaagcg
     1801 ggcttcggct gccgccgggc gcatcgcgcg ggcgcgtagc gccaggtagg tgagcaggat
     1861 cattgtcgcc ccggccgcga gcagcgcact gcgcaacagc ccgccacgct gcggctcggc
     1921 gaggccgatg taaatcagcg tgtcgtcgac ggcaacggca atccccacgg ccaatgcgac
     1981 gacgaagccg gtcgcggcgt agcccggcag acgcgacagc ggccgtgtca gcaagacgca
     2041 gaggccgagc gtaccgagca gcacgggaac gacgtggatt gcgttggcga cgaagacctg
     2101 cggccaatcg gctatgcgcg gcgcgccgac gagcgccgcc gcggcggcaa tgacgacgcc
     2161 gccgagcagg atgcgcaagg ctatgccgag attgcggaaa tccggcagca gcggggcttc
     2221 aggcaattcg cgtatacttt tatccataat ccgtgcgccg gaatcctcgg gttccgccgc
     2281 cccggccagc ggcaggcgag cgctgccatg tttgccgcgg tgtcgatcgg ggccggccca
     2341 ttctaatcaa cttgtcgagt cttacgcatg agtcattccg acgctgccaa tcccgcctac
     2401 acttgggcgg gccgcttttc cgaaccggtt tccgatctcg tcaaacgcta cacggcgagc
     2461 gtcgatttcg accagcgcat gtggcggcag gacatcgccg gctcactggc ccacgccaag
     2521 atgctggcga aacagggaat catcggcacg gacgacctgc aggcgatcga aggaggcatg
     2581 gcaacgatcg tcagcgaaat cgagaccggc cagttcaagt ggtcggtcga tctcgaagac
     2641 gttcatttca acatcgaaaa gcgcctgacg acgcttatcg gcgattccgg caagcgcctg
     2701 cacaccggcc gttcgcgcaa cgatcaggtg gcgaccgaca tccgcctctg gctgcgtgac
     2761 gcgatcgaca ccatcgtcgg cctgctcggc gactaccagc gcgcgctgat cgatctcgcc
     2821 gagcagcacg cggcgacgcc gatgccgggc tttacccacc tgcaggtagc gcagccggtc
     2881 acctttggcc accatctgct cgcctacgtc gagatggcgg agcgcgaccg cgagcgcatg
     2941 cgcgattgcc gccggcgcac caaccgcctg ccgctcggcg ctgcagccct tgccggcaca
     3001 acctatccga tcgaccgcga gttcgtggcg aaggaactcg gcttcgaggg cgtctgcgag
     3061 aactcgctcg acgcggtttc cgatcgcgac ttcgccatcg agttctgcgc cgccgcggcg
     3121 ctggtgatga cgcacgtctc gcgcttctcc gaggagctag tgatctggat gagcccgcgt
     3181 gtcggcttcg tcaggatcgc cgaccgcttc tgcaccggca gctcgatcat gccgcagaag
     3241 aaaaaccccg acgtacccga gctggcgcgc gggaagaccg gccgcgtcaa cggcgacctg
     3301 atcgcgctgc taacgctgat gaagggccag ccgctcgcct acaacaagga caaccaggaa
     3361 gacaaggaac cgctgttcga cgccgccgac acggtgatcg acacgctgcg catcttcgcc
     3421 gacatggtcg gcggcatcac gccgcagcct gaagcgatgg cggcggcgct gacccaggga
     3481 tttgccaccg cgaccgacct cgccgactac ctggtgaaga agggcctgcc cttccgcgat
     3541 gcgcacgagg ccgtcgggct cgccgtccgc gccgccgaaa cacgcggcgt agacctgccg
     3601 caactgccgc tcgaggaact gcgccgcttc tcgccgctgg tcggcgagga tgtgttcgcg
     3661 gtgctcaccg tcaccggctc gctggcctcg cgcaagcact ttggtggcac ggcgcccgag
     3721 caggtgcgcg cggcgatcgt gcgtgcccgc gcccggctcg gctgaagcag ttcgcccgat
     3781 ggatcgcctc ggcaagtacg agatcgagcg cgaaatcggt cgcggtgcaa ctagcgtcgt
     3841 ctttctcggc aacgatccgt tcgcgcagcg tcaggtagcg atcaaggtcg gctaccccga
     3901 catcctcaag gacgccgagc gcggcaaggt ctactcgcac ctgttcatca acgaggcctc
     3961 gctggtcggc aagctgcagc acccgcacat tgtgcagatc ttcgacgcgg tggtcggaga
     4021 aggtgtcgcc tacatcgtta tggagtacgt gcccggcacg acactcgagg ccttctgcaa
     4081 gcccgacacg ctgctttcct tcgagcgcat cgttgagatc atcttcaagt gcacgcgggc
     4141 gctcaacttc gcctgccagc tcggcatcac gcaccgcgac atcaagccgg cgaacatcct
     4201 gatcgcgcag tcggaaaccg gcttcggcga catcaagatc tccgatttcg gcgcggcgat
     4261 caccacttcg accgaccgca cgatggtctc ggggatcgga tcgccggcct acatgtcgcc
     4321 gcagcaggtg cgcgaggagt cgctcgatct gcgcactgac atctattcgc tgggcgtggt
     4381 catgtaccag ttgctaaccg gccaactgcc gttccaggcg agcagcaact acaacatggt
     4441 ctaccagatc atccataccg acccgacgcc acccgagcgc ctgcgcgacg aagtgccgca
     4501 gcggctcgct gacatcgtcg ccaaggcgat gtccaagcgt atcgagtcgc gttaccagac
     4561 ttgggaggaa ttcgcgcagg acctcgcgca ggccttccgc gcgcgcagca acgagaaatc
     4621 gcgcttcgcc gacagcgaga agttcagcac gctgcgcgca ctgcccttct tcgagcgttt
     4681 ctcggacgtc gagatttggg aagctgtccg cttctcgcag tgggagcacg tgcgccccgg
     4741 tcacgcgatc atgaaggacg gcgaggacgg cgacttcttc tgctttctgg ccgagggcga
     4801 agtcaaggtc gccaagaacg gcaagacgct caacgtgctt tcagcaggcg actgtttcgg
     4861 cgaaatggcg gttatcggcc aagcttcgca cgtgcgcggc gccgacgtgg tggcactcgg
     4921 cgacgccaag gtaatccgca tccccggcga agcgctgcac gccgccagcg aatcgtgccg
     4981 cctgcagttc taccagtcct tcctcgaagt gttggccaac cgtctgacgc tcgccaacac
     5041 gcggctcgcc gccttctgaa ggctgcgcgc cgcgcctcgg acctgaacgc ggcctcaggc
     5101 cgatttctgg tcggcgaact cggcgagcag ctgctgcaac tcgccggcat tgaacatctc
     5161 tttcatgatg tcgtggccac caacaaattc gcccttgacc cagaactgcg gcagcgtcgg
     5221 ccagttggcg aactcgcgca gcccggcaac cagcgctgga tcaggcagca cgttgacagc
     5281 aaagaagttc ttcacgccgc aacgctggat catctgcgcg gcgttcgccg agtagccaca
     5341 ctgcggaaac tgcggcgttc ccttcatgta gagcacgacc gggtgtgtgc tgatctgctg
     5401 gcgaatcgtt tcctggatat ctgacatcgt cttgctccgc ttgattgacc cggaaacaga
     5461 ccgccatcgg aggccgaagt tcaaggcctc gcggccttgc ggtgaggctc aggctggtgg
     5521 ctgccagcgg ccgccgctga cccgctcgtg gcccgacagg tcgcgccggc tggcgatggc
     5581 gatgaatccc gccctggcca gcagggcgcg caccgcggcc gcctgatcgt agccatgttc
     5641 gagcagcagt tgtccgcccg gcgccaggaa ggccggcgca ccggcgatga tctcgcgcag
     5701 acaggcgagc ccatcgccgc cgtcggtgag cgccaggtcg ggctcgaagg gcagtccgtc
     5761 ctgcgccaga tgtggatcgc cggcggcaac gtagggtgga ttggagacga ttaggtcgaa
     5821 ctgggtcggg ccaagagccg cgtaccagtc gctggcgaca aaggagacgg tcgcgccgag
     5881 taccgcggca ttgcgaccgg caaccgtaag cgccgccgcc gatagatcgg tggcggtcag
     5941 gcgtgcaccg ggcaattcga gcgccagcgt gaccgcgatc gcgccgctgc cggtgccgag
     6001 atcgagaatc gccgcctcgc cgccccgctc ctccgccacc tcgatggcag cgaggaccag
     6061 ccgctcggta tcgggcctcg gaatcagcac gtccgcggtg acggcaaaac tgcgcccgaa
     6121 gaattcacgc tcgccggtca ggtaggcgag cggctcgccg ttggcgcggc ggttgaccag
     6181 cgccatgaag gcgtttgcct cgtcgtggct caggcgccgc tcgggatggg caaggaattc
     6241 ggcgtgaccg atgccgacga tgtgctgaac caggagccgc gcatcgagac gggcgatgcg
     6301 ccggcgcacg gcatcgacgg cttcgcggac ggtgaccgtg ccatccgtgc cggtcgccgc
     6361 caccaccgtc acaccgtttc ggcgagcgcc gccagctgct cggcctggta ctcggcggcg
     6421 agcgcgccga gcaactcgtc gagatcgccg tccatgatct gcgcgatctt gtagagcgtc
     6481 aggttgatgc ggtggtcggt aatgcgaccc tgcgggaagt tgtaggtgcg gatgcgctcg
     6541 gagcggtccc ccgatccgac caggctcttg cgcgccgctg cctccttcgc ctgcgcatcg
     6601 cgggtctgct tgtccttgag tcgcgccgcc agcacgctca tcgcctgcgc gcggttccga
     6661 tgctgcgagc ggtcgtcctg gcattcgacg acgaggccgc tcggcaggtg ggtgatgcgc
     6721 accgccgaat cggtcttgtt cacgtgctgg ccaccggcac ccgacgcgcg gaaggtgtcg
     6781 atgcgcagat cggccgggtt gatgtcgatc tctgctaact cgtcggcctc gggcatcacc
     6841 gcgaccgtgc aggccgaggt gtggatacga ccctgcgcct cagtctcggg aacccgctgg
     6901 acgcggtggc cgcccgactc gaacttgagc cgcgagtaga cgccggtgcc tgtgacgcgg
     6961 gcgatcacct cgcggtagcc gccgaggtcg gattcgctgg ccgagacaat ctcgacgcgc
     7021 cagcgctggc gttcggcaaa gcgcgaatac atgcgaaaaa gatcggcagc gaacagcgcc
     7081 gactcgtcgc cgccggtacc ggcgcgtatt tcgagaaaga cgttgcgctc gtcgttggcg
     7141 tccttcggca gcaggagctt ctgcagttcg aaatcgatga ccggcagcgc cgcttcgcgc
     7201 tcggcgattt cctcggcggc catctcggcc atctcggggt cggcaagcat cgctcgcgct
     7261 tcgccgatgg cggcttcggt ggcgcagaaa cgggcaaata gcgtcaccac cggttcgacc
     7321 tcggcgcgct cctgcgacag gcggcgcagt gcgtcggggt tggcggcggt ctccgggctc
     7381 gacaacagtg aatcgagctc ttcgaggcgc atggcgagcc gctcgaggcg atcgcgcagg
     7441 gtcggtttca tcggcgtggg caatccgggc tattcgccgg gatgcagatg aaagaggcgc
     7501 gccgccgcct gctgcagttc gtcccggtgt tcggggtcgg cctggttcag cgcctgcgtc
     7561 ggcgcgtgca ggaatttgtt ggtgagccgc tgcgacagca attcgacgat tttctcgacc
     7621 ggttcgccac gggcaatcag cttcatcgca tggtcgagct cgtgccggcg catgcgctca
     7681 gccgagtctc gcaggctgcg gatcgtcggc accgtcgtgc tgcgcgtttc cagccagtgc
     7741 acgaaaccgt cgacgcgatt ggtgatgatt gcctcggctt cgaccacggc ggcctgccgc
     7801 gact
//
LOCUS       DATDAA010000103         6741 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_77167,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000103 DATDAA010000000
VERSION     DATDAA010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77167"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..768
                     /locus_tag="VIS73_03440"
     CDS             274..768
                     /locus_tag="VIS73_03440"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY5762239.1"
                     /translation="MTKTTNILVLCTGNSARSILGEALFNHHGKGRVRAFSAGSQPSG
                     KVNPVAVETLEKHGIALPELRSKSWDEFATPGSPVIDFIFTVCASAAGEACPVWPGKP
                     ATANWAIPDPAHVEPIEKRREAFERAYQQLDQRIRAFLAMDLSEMSRKDISTAAKMIH
                     EACQ"
     gene            765..1865
                     /gene="arsB"
                     /locus_tag="VIS73_03445"
     CDS             765..1865
                     /gene="arsB"
                     /locus_tag="VIS73_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEY5762240.1"
                     /translation="MSAQCEVIGKQAAGAPMSMFERYLTVWVFLCIVAGIALGQFAPG
                     FFQAVGRMEVARVNLPVGLLIWVMIIPMLVKVDFGALHEVKSHVRGIGVTLFVNWLVK
                     PFSMAFLGWLFIRHLFAPYLPAEQIDSYIAGLILLAAAPCTAMVFVWSRLSNGDPLFT
                     LSQVALNDTIMVFAFAPIVAFLLGLSAIVVPWDTLLTSVVLYIVIPVILAQLWRRRLL
                     RGGQAAFDSAMARIGPWSIAALLATLVLLFAFQGEAILAQPLIIALLAVPILIQVFFN
                     SALAYWLNRALGEKHSVACPSALIGASNFFELAVAAAISLFGFESGAALATVVGVLIE
                     VPVMLLVVRVVNSSKGWYERAGTAAVERPPSA"
     gene            complement(1855..2259)
                     /locus_tag="VIS73_03450"
     CDS             complement(1855..2259)
                     /locus_tag="VIS73_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762241.1"
                     /translation="MPHASSTTSPHQRSAHPAGELRRLLLGRGLRAFCDGYVAILLPV
                     YLLALGLDPWSVGAISSATLLGSALMTLAIGQHGHRYPSRGLLLAGASPALGGALFAA
                     GWLAAPLLLFGGLKIAYDLAIWRAFRGVTVRR"
     gene            complement(2276..2593)
                     /locus_tag="VIS73_03455"
     CDS             complement(2276..2593)
                     /locus_tag="VIS73_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY5762242.1"
                     /translation="MANGITAAALQAEAAQLTLVDVRRVAARDASPEAIPGASWRDPE
                     RVGEWAADLPAGRRIVVYCVHGHEVSQGVADALVASGHEARYLDGGIVGWVVAGGPVT
                     GKA"
     gene            complement(2631..3974)
                     /gene="chrA"
                     /locus_tag="VIS73_03460"
     CDS             complement(2631..3974)
                     /gene="chrA"
                     /locus_tag="VIS73_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEY5762243.1"
                     /translation="MTETAIPDTSLPTPTLADAFRYWLKLGFISFGGPAGQIAMMHQE
                     LVERRRWISERRFLHALNYCMLLPGPEAIQLAIYIAWLMHGIGGAIVAGVLFFLPAFA
                     LLSLFAGIYLAYGDVALVQGIFYGIKPAVVAVVLFAAWRIGSKAIRNRLLGGIAAMAF
                     VGIFFFRIDFPWIVLAAGLLGVLGGRLAPDKFTGGGGHGASDKRYGPAIIDDQTPPPA
                     HAAFSWRKLAVTLAIFVGLWVVAMVALRGSTSLSEMGSFFTGAAFLTIGGAYAVLPYV
                     YQGAVEQFGWLTGPQMIDGLALGETTPGPLIMIVTWVGYIGGVTKGVLGDPIAAGLAG
                     AAVATFFTFLPSFLFIIAGGPMVEATRNELKFTAPLTAITAAVVGVILNLAVFFAWHT
                     FWPRASDADPFAAPFEWLPVLVAAASFVALWRYKQDVMRVIGVCAVLGLALSFVR"
     gene            complement(4154..5479)
                     /gene="phoR"
                     /locus_tag="VIS73_03465"
     CDS             complement(4154..5479)
                     /gene="phoR"
                     /locus_tag="VIS73_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEY5762244.1"
                     /translation="MTRRTGLHPVWWAFIGRIAAITVVALVVWLSAGPDWALAVAVLL
                     LLVMMASRTQHMAFLARWIDGSDERDFPQAGGAWGEVFLALSRRLRWEAQEMARVHAE
                     AAAFREAMAALPDGIVLVNGLSQIVWCNSAAEQHMGINREKDKGRVITHLVRMPGFAE
                     YLQLNDRAEPFSFTSVGDRNVVLGLEVVPVGNGQQLLVSFDVSEIRQAEAMRRDFVAN
                     VSHELRTPLTVIHGFLEHLAGDALPEADRRQFVGLMQDQSARMLTLVDDLLTLSRLES
                     ERLPRREELIDMDALVARLVDEAKLLSNGRHEISAQIAPVDLRGDPDELRSAFANLLS
                     NAVRYTPEGGKISVEWEDSLGGPTFSVADTGIGIAAEALPRLTERFFRVDKGRSRETG
                     GTGLGLAIVKHVLIRHQADLRILSTPGEGSIFAARMPVERCVRRSTSSS"
     gene            complement(5476..6180)
                     /gene="phoB"
                     /locus_tag="VIS73_03470"
     CDS             complement(5476..6180)
                     /gene="phoB"
                     /locus_tag="VIS73_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEY5762245.1"
                     /translation="MKAGPGTTVLIVEDDPAIRALLRYTLQQANFAVTEASTAEEGLR
                     LLGDVLPDVVLIDWMLPGMSGVTMARQLRQDKRTKDLPLIMVTARGDEADRVAGLEQG
                     ADDYLVKPFSPRELLARIQAVLRRRAPEIAETVLEVGPLRLDPVEHALSCEGKPVNLA
                     LAEFKLLRFLMANPGRVFSRGQILDQVWGDHVFIEERTVDVHIRRLRAGLGPLGERMI
                     ETVRGIGYKLVPPGQS"
     gene            complement(6177..>6741)
                     /gene="phoU"
                     /locus_tag="VIS73_03475"
     CDS             complement(6177..>6741)
                     /gene="phoU"
                     /locus_tag="VIS73_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY5762246.1"
                     /translation="AGGDDRVDRYEVDIDEACTRLIAKRQPTAGDLRLVMAVARIVTD
                     LERCGDKAAKIARMSSKLLRGRDGAPPANGVVPHLSNLKRFGEAAIAMLRKALDALAR
                     LDADAAEKVIREDVEVDENFESIMRELITYMMEDSRTISRGLDIVWIAKAFERVGDHA
                     ANIAENVVYIVRGEDVRHQGSDIEGDA"
BASE COUNT         1186 a         2290 c         2177 g         1088 t
ORIGIN      
        1 ccgcgagcgc gagtcgaata gattgttgaa caggcgtaga cagcattggc gtctcccaag
       61 tcgatggcgc cgtaccgtcg gcgccgaaac gatcgtgagt ctagtgcagc gggcccggag
      121 gatttgcgac cgcgatcact aaccgcgtgg gaggagcgcg aatcaagcgc ccggcttccc
      181 ttctgcgctg ctgcccagcg tcaaaacggt gcgggccgac gccgaaaccg gcaaaatcgc
      241 cccttgatac cgaatgtagg acgcttcccg cgcatgacca agacgaccaa catcctcgtg
      301 ctatgcaccg gcaactcggc ccgctcgatt ctcggcgagg cactcttcaa tcatcacggc
      361 aagggtcgcg tgcgagcctt ttccgccggt agccagccct cgggcaaggt caatccggtc
      421 gccgtcgaga cgctcgaaaa gcacggcatt gcgttgcccg aactgcgcag caagtcctgg
      481 gacgagttcg ccaccccggg ttcgccggtg atcgacttca tcttcaccgt ctgcgcctcg
      541 gcagccggcg aggcctgccc ggtctggccg ggcaagccgg caacggcgaa ctgggcgatc
      601 cccgacccgg cacacgtgga gccgatcgag aaacggcgcg aggccttcga gcgcgcctac
      661 cagcagctcg accagcgtat ccgcgccttc ctggcaatgg atctgagtga gatgtcgcgc
      721 aaggacatct ccaccgcggc aaagatgatc cacgaggcct gccaatgagc gcccaatgcg
      781 aagtgatcgg caagcaggca gccggcgcgc cgatgtcgat gttcgagcgc tacctgacgg
      841 tgtgggtgtt cctctgcatc gtcgccggca ttgcgctcgg ccagtttgcc cccggattct
      901 ttcaggccgt cggccgcatg gaggtcgctc gggtcaacct cccggtgggg ctgctgatct
      961 gggtgatgat cattccgatg ctggtcaagg tcgatttcgg cgcgctgcac gaggtcaaga
     1021 gccacgttcg cggcattggc gtgacgctgt tcgtgaactg gctggtcaag ccgttttcga
     1081 tggccttcct cggctggctg ttcatccgcc atctgttcgc gccgtacctg ccggccgaac
     1141 agatcgacag ctatatcgcc ggcctcatcc tgctcgccgc cgcgccctgc acggcgatgg
     1201 tcttcgtctg gagccggctg tcgaacggtg acccgctgtt cacgctgtcg caggtcgcgc
     1261 tcaacgacac gatcatggtc ttcgcctttg cgccgatagt cgccttcctg ctcggcctct
     1321 cggcgatcgt cgtgccttgg gacacgctgc tgacctcggt cgtgctctac atcgtcattc
     1381 cggtgatcct tgcgcaactc tggcggcgcc ggcttctccg cggcggccag gccgccttcg
     1441 attcagccat ggcacggatc ggcccgtggt cgattgcagc actgctggca acgctggtgc
     1501 tgctcttcgc cttccagggc gaggcgatcc tcgcccagcc gttgatcatc gcgcttctcg
     1561 cggtaccgat cctgatccag gtgttcttca actcggcgct cgcctactgg ctcaaccgcg
     1621 cgctgggcga gaagcactcg gtcgcctgcc cgtcggcgct gatcggcgcc tcgaacttct
     1681 tcgagctggc ggtggccgcg gcgatcagcc tgttcggatt cgagtcgggc gcggcgctgg
     1741 cgaccgtggt cggcgtgctg atcgaagtgc cggtgatgct gctggtcgtg cgcgtcgtca
     1801 attccagcaa aggctggtat gaacgcgctg gaacggcggc tgtggagcgg ccgcctagcg
     1861 cctgaccgtg acgccgcgga aggcgcgcca gatcgccagg tcgtaggcga tcttcaaacc
     1921 accaaagagc agcagcggcg cggccagcca gccggccgca aacagcgcac cgccgagcgc
     1981 cggactggcc ccggcgagca gcaggccgcg cgacggataa cggtgaccgt gctggccgat
     2041 ggccagcgtc atcagcgccg agccgagcag cgtcgcgctg ctgatcgcgc cgaccgacca
     2101 ggggtcgagg cccagcgcca gcagatagac cggcagcagg atggcgacgt agccgtcgca
     2161 gaaggcgcgc aggccgcggc cgagcagcag gcggcgcagc tcgcccgccg ggtgcgcact
     2221 gcgctgatgt ggcgaggtgg tcgatgaagc gtggggcacc gcggcgcgcc cttgcttatg
     2281 ccttgccggt gaccgggccg ccagctacca cccagccgac gattccgccg tcgaggtagc
     2341 gcgcctcatg gccactggca acaagggcgt cggcgactcc ctggctgact tcgtggccgt
     2401 gcacacaata aacgacgatt cgccggccag ccggcaggtc ggcagcccac tcgcctaccc
     2461 gctcagggtc gcgccagctc gcgccgggaa tggcctcggg tgaagcatca cgcgcggcga
     2521 cgcgccgcac gtcgaccagc gtcagctgcg cggcttcggc ctgcagagcg gcggccgtga
     2581 tgccgtttgc catttccagt ctcctgttat cggttgcagc gaaacgccgt tcagcgcacg
     2641 aaggacagcg ccagaccaag caccgcgcag acgccgatga cgcgcatcac atcctgcttg
     2701 tacctccaca gcgcgacgaa ggatgccgcg gcaacgagca ccggcagcca ctcgaagggc
     2761 gcggcaaacg ggtcggcgtc gctcgcccgc ggccagaagg tgtgccaggc gaaaaacacc
     2821 gcgaggttga ggatgacacc gaccacggcg gccgtaatgg cggtcagcgg cgcggtgaac
     2881 ttcagttcgt tgcgcgtcgc ctcgaccatc ggcccgccgg cgatgatgaa caggaagctc
     2941 ggcaggaaag tgaaaaacgt tgccaccgcc gccccggcaa gccccgccgc gatcgggtcg
     3001 ccgagaacgc ccttggtcac gccgccgatg tagccgaccc aggtgacgat catgattagc
     3061 gggcccggcg tggtttcgcc gagcgccagc ccgtcgatca tctgcggccc ggtgagccag
     3121 ccgaactgct cgacggcacc ctggtagacg tacggcagca cggcgtaggc gccgccgatg
     3181 gtcaggaacg ccgcgccggt gaagaacgat cccatctcgg acagcgacgt cgagccgcgc
     3241 agcgccacca tggcgacgac ccagagcccg acgaagatcg ccagcgtcac ggccagcttg
     3301 cgccacgaga aagccgcgtg cgccggcggt ggcgtctggt cgtcgatgat tgccgggccg
     3361 tagcgcttgt cgctggcgcc gtggccaccg ccgccagtga acttgtccgg tgccagtcgg
     3421 ccgccaagga cgcccagcag cccggcggca agcacaatcc acgggaagtc gatgcggaag
     3481 aagaatatgc cgacgaaggc catcgcggcg attccgccga gcaggcgatt gcggatcgcc
     3541 ttcgaaccga tccgccaggc cgcgaagagc acgacggcga ccacagccgg cttgatgccg
     3601 tagaagatcc cctgcaccag cgcgacatcc ccgtaggcga ggtagatgcc ggcaaacagc
     3661 gagagcagcg cgaatgccgg caggaagaac agcacgccgg cgacgatggc gccgccgatg
     3721 ccatgcatca gccaggcgat gtagatcgcc agctggatcg cctcgggacc cggcagcagc
     3781 atgcagtagt tgagcgcatg gaggaaacgc cgctcggaga tccagcgacg gcgctcaacc
     3841 agctcctgat gcatcatcgc gatctgcccg gccggcccgc cgaagctgat gaagccgagc
     3901 ttcagccagt agcgaaaagc atcggccagc gtcggcgtcg gtagcgacgt atctggaatg
     3961 gcagtttcgg tcataagcct gccttgcatt tgagatgcag ctgccacgtc gagaaccgtc
     4021 gccgcgtcat cgctgccttg gggatgcgcc gctgccacaa cgaggaatct tggtgcgcag
     4081 ccaaccagac tgtattacag attccagacg ccgcgccggc ctcgcccgcg tgagcatccg
     4141 tttccttcta tcgctacgac gagctcgtcg aacgccgcac gcaacgctcg accggcatcc
     4201 gtgccgcaaa gatactgcct tcgcccggcg tcgagagaat ccgaaggtct gcctggtggc
     4261 gaatcagcac gtgcttgacg atcgccagcc cgaggccggt accgcccgtc tcgcgcgaac
     4321 ggcccttgtc gacgcggaag aagcgttcgg tcagacgcgg cagcgcttcg gcggcgatgc
     4381 ctattccggt gtcggcaacg gagaaggtcg gaccgccgag gctgtcttcc cattcgaccg
     4441 atatcttccc gccctccggc gtgtagcgca cagcgttgga aagcaggttg gcaaaggcgc
     4501 tgcgcaactc gtcgggatcg ccgcgcagat cgaccggcgc gatctgcgcg ctgatctcgt
     4561 ggcggccgtt cgacaacagc ttcgcctcgt cgaccagtcg ggcaaccaac gcatccatgt
     4621 cgatcagttc ctcgcgtcgc ggcaggcgct cgctctccag ccgtgacagc gtcaagaggt
     4681 cgtcgaccag cgtcagcatg cgcgccgact ggtcctgcat cagcccgaca aactgccggc
     4741 ggtcggcctc tggcagcgcg tcgccggcaa ggtgctcgag aaagccgtgg atgaccgtca
     4801 gcggcgtgcg cagttcgtgc gacacgttcg cgacaaaatc gcggcgcatg gcctccgctt
     4861 gccggatttc cgaaacgtcg aagctcacca gcagttgctg gccattgccg accggcacca
     4921 cctcgagtcc aagcacgaca ttgcggtcac cgacgctggt aaagctgaac ggctcggccc
     4981 gatcgttgag ttgcaggtat tcggcgaatc ccggcatacg gaccaaatgc gtaatgacac
     5041 gccccttgtc cttctcgcgg ttgattccca tatgctgctc ggcagccgag ttacaccaga
     5101 cgatctgaga caggccgttg accagcacaa tgccatccgg aagcgctgcc atcgcctcgc
     5161 ggaaggcggc cgcctcggca tgtacccgcg ccatctcctg cgcctcccag cgcagccggc
     5221 gcgacagcgc cagaaagacc tcgccccagg cgccgccggc ttgcgggaag tcgcgctcgt
     5281 cgctcccgtc gatccagcgg gccagaaagg ccatgtgttg cgtgcgcgag gccatcatca
     5341 ccaacagcag gagtacggcg acggccaacg cccagtcggg cccggcagat agccagacga
     5401 ccagcgcgac gacggtgatt gcggcgatgc gcccgatgaa ggcccaccag accggatgca
     5461 atccggttcg ccgggtcacg attgacccgg gggaaccagc ttgtagccga tgccgcgcac
     5521 cgtttcgatc atgcgctcgc caagcgggcc gaggcctgcg cgcaggcggc gaatgtgcac
     5581 gtcgacggta cgttcctcga tgaagacgtg atcgccccag acctggtcga gaatctggcc
     5641 gcggctgaag acgcggcccg gattggccat caggaagcgc agcagcttga attcggcaag
     5701 cgcgaggttc accggcttgc cctcgcagct aagcgcatgc tcgactgggt caaggcgcag
     5761 cggcccgacc tcgaggacgg tctcggcgat ctccggcgcg cgacgacgaa gcaccgcctg
     5821 gattcgcgcc agcagttcgc gcggactgaa gggcttgacc aagtagtcgt cggcgccctg
     5881 ctcgaggccg gcgacccgat cagcctcgtc accgcgggcg gtgaccatga tcagcggcag
     5941 gtccttggta cgcttgtcct gccgcaactg cctagccatc gtcacgccgc tcatgccagg
     6001 cagcatccag tcgatcaaaa cgacgtcggg cagcacgtca cccaggagtc gcagcccctc
     6061 ctcggccgtc gacgcctcgg tcactgcgaa gttggcctgc tgcagcgtat agcgcagcag
     6121 cgcccggatc gccgggtcgt cctcgacgat caagaccgtc gtgccgggtc cagccttcac
     6181 gcatcgccct cgatatcgct gccctgatgg cggacgtctt cgccgcgaac aatgtagacg
     6241 acgttctcgg cgatgttggc ggcgtgatcg ccgacgcgct cgaaggcctt ggcgatccag
     6301 acgatgtcga ggccgcggga aatcgtccgc gaatcttcca tcatgtaggt aatcagctcg
     6361 cgcatgatgc tttcgaagtt ctcgtcaact tcgacatcct cgcggatgac cttctccgcc
     6421 gcatcggcat cgagccgcgc cagcgcatcg agcgccttgc gcagcatcgc gatcgccgct
     6481 tcgccgaagc gcttgaggtt ggacaggtgc ggcacgacgc cgttggccgg cggtgcaccg
     6541 tcgcgcccac ggagcagctt gctcgacatg cgcgcgattt tcgcggcctt gtcgccgcaa
     6601 cgctcgaggt cggtgacgat tcgcgccact gccatcacca gtcgcaaatc gcccgccgtc
     6661 ggctggcgct tggcgatcag gcgggtgcag gcctcgtcga tgtcgacttc gtaacggtcg
     6721 acacggtcat cgccgccagc g
//
LOCUS       DATDAA010000104         2593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_462018,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000104 DATDAA010000000
VERSION     DATDAA010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462018"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..2519
                     /locus_tag="VIS73_03480"
     CDS             117..2519
                     /locus_tag="VIS73_03480"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEY5762247.1"
                     /translation="MAASPDSIERETQLQGMGVSRLRKEDARFIRGKGTYIDDIKMPG
                     MLFGAIVRSPYAHARIKSVNKEKALALEGVVAVLTADDLKPVNLHWMPTIGGDSQAVL
                     ADEKVCFQMQEVAMVLATDRYVAADGADLVEVDYEELPAIVDPHKAMDADAPVIREDI
                     ADKDEVGHGKRTHPNHIFTWESGDKETTDAAFDNAEVTVREEILNPRVHPCPLETCGC
                     VASFDKPTGKLTVHITSQAPHLVRTVVAMLSGIPESKIRIIGGDIGGGFGNKVPIYPG
                     YVIAIVASIVTGVPIKWIESRMDNISTTGFARDYHGIGELAADKDGRIKALRFSALAD
                     HGAFNSHVSATKLPAGLFNICTGSYDIPAAYCRVDAVYTNKAPGGVAYRCSLRVTEAV
                     YLIERMMDVLAQKVGVDKAEIRRRNFIRKDMFPYNTAFGWTVDSGDYDTALDKVLEAV
                     DYEGLRKEQAEKRARGELMGIGISTFTEIVGAGPTKVCDILGIGLFDSCDIRVNPTGS
                     AIARLGTMTQGQGHATTYAQIIATELGLPSDDIQIEEGDTDTAPYGAGTWGSRSTPVA
                     GAAAALAARKIKAKAQKIAAHLLEVGEADLEWEIDRFQVKGNPSQARTMKEIALVAHT
                     GDLPEGMEQGLGASEYYDPPNMTYPFGTYVCVVDIDRHTGETTVRRFYALDDCGTRIN
                     PMIIEGQIHGGLTEAFAVAFGQQIPFDESGNHLGNTLMDYFLPTSAETPHWETDETVT
                     PSPHHPIGAKGVAESPHVGGIPCFSNAVVDAFAHLGVTHMDMPHNAFRVWKQLKAHGV
                     NL"
BASE COUNT          501 a          887 c          836 g          369 t
ORIGIN      
        1 tggccggcaa cctgtgccgc tgcacggggt atcagaacat cgtcaaggca gtgcggtacg
       61 cggcagaaaa gctgtcggcg caggcctgac ccacggactt tcagggagat attttcatgg
      121 ctgcttcacc cgattcgatc gaacgagaga cacagctgca aggcatgggc gtttccaggt
      181 tgcgtaagga agacgcccgt ttcattcgcg gcaagggcac ctatatcgac gacatcaaga
      241 tgccgggcat gctgttcggc gctatcgtcc gcagtcccta tgcgcatgcg cgcatcaagt
      301 ccgtcaacaa ggagaaggcg ctcgcgctgg aaggcgtcgt cgcggtcctc acggcggacg
      361 acctgaaacc ggtcaacctg cactggatgc cgaccatcgg cggcgactcg caggcggtgc
      421 tggcggacga gaaggtctgc ttccagatgc aggaggtggc gatggtgctc gccaccgatc
      481 gctacgtcgc ggcggacggc gccgacctgg tcgaagtcga ctacgaagag ctgccggcca
      541 tcgtcgatcc gcacaaggcg atggacgccg acgcgccggt catccgcgag gacatcgccg
      601 acaaggacga ggtcggtcac ggcaagcgca cgcacccgaa ccacatcttc acctgggaat
      661 cgggcgacaa ggaaaccacg gacgccgcgt tcgacaacgc ggaggtgacc gtaagggaag
      721 agatcctgaa cccgcgcgtg cacccctgtc cgctggagac ctgcgggtgc gtcgcgtcct
      781 tcgacaagcc cacgggcaaa ctgaccgtgc acatcacctc gcaggcgccg cacctggtgc
      841 gcacggtcgt cgccatgctg tccggcatcc cggagagcaa gatccgcatc ataggcggcg
      901 acatcggcgg cggcttcggc aacaaggtgc cgatctaccc cggctacgtc atcgccatcg
      961 tcgcctcgat cgtcacgggc gtgccgatca agtggatcga gtcccgcatg gacaacattt
     1021 cgacgacggg attcgcccgc gactaccacg gcatcggcga gctcgcggcc gacaaggacg
     1081 gccgcatcaa ggcgctgcgc ttctcggcgc tcgccgacca cggcgcgttc aactcgcacg
     1141 tgtccgcgac caagctgccg gccgggctgt tcaacatctg cacggggtct tacgacattc
     1201 ccgcggcgta ttgcagggtc gacgccgtct acaccaacaa ggcgcccggg ggcgtcgcct
     1261 accgttgctc gctgcgcgtc accgaagcgg tgtacctgat cgagcgcatg atggacgtgc
     1321 tggcgcagaa ggtcggggtc gacaaggcgg aaatacgccg gcgcaacttc atccgcaagg
     1381 acatgttccc ctacaacacc gccttcggct ggaccgtcga cagcggcgat tacgacacgg
     1441 cgctcgacaa ggtcctcgag gccgtcgact acgaggggct gcgcaaggag caggccgaga
     1501 agcgcgcccg gggcgagctg atgggcatcg gcatcagcac tttcaccgag atcgtcggcg
     1561 ccgggcccac caaggtatgc gacatcctgg gcatcggcct tttcgacagc tgcgacattc
     1621 gcgtgaaccc gaccggcagc gcgatcgccc gcctgggcac gatgacccag gggcaggggc
     1681 acgccacgac ctatgcgcag atcatcgcca cggaactcgg cctgccgtcc gacgacatcc
     1741 agatcgagga aggcgacacc gacacggcgc cctacggcgc gggcacctgg ggctcgcgct
     1801 cgacgccggt cgccggcgcg gccgcggcgt tggccgcacg caagatcaag gcgaaggcgc
     1861 agaagatcgc ggcgcacctg ctcgaagtcg gcgaggccga tctcgagtgg gaaatcgacc
     1921 gcttccaggt caagggcaac ccgtcgcaag ccaggacgat gaaggaaatc gccctggtcg
     1981 cgcacaccgg cgacctgccc gagggcatgg agcaggggct cggcgcgtcc gaatactacg
     2041 acccgccgaa catgacctac ccgttcggca cctacgtctg cgtcgtcgac atcgaccgcc
     2101 acaccggcga gaccacggta aggcgcttct acgcgctcga cgactgcggc acgcggatca
     2161 acccgatgat catcgagggc cagatccacg gcggcctcac agaggcgttc gccgtcgcct
     2221 tcggccagca gatccccttc gacgagagcg gcaaccatct cggcaatacc ctgatggact
     2281 atttcctgcc gacctccgcg gaaacgccgc actgggagac cgacgaaacg gtcacgcctt
     2341 cgccgcatca cccgatcggc gccaagggcg tcgccgagtc gccgcacgtc ggcggcatac
     2401 cgtgtttctc caacgcggta gtcgacgcgt tcgcgcacct gggcgtcacc cacatggaca
     2461 tgccgcacaa cgcgttccgg gtctggaagc agctcaaggc gcacggcgtg aacttgtaac
     2521 cccccccgcc gcgctgcgcg cgccccccag ggagcggcgc tggcgggccg gcggagccgg
     2581 acccgcggcg ccc
//
LOCUS       DATDAA010000105         3046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_112864,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000105 DATDAA010000000
VERSION     DATDAA010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112864"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VIS73_03485"
     CDS             complement(<1..737)
                     /locus_tag="VIS73_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762248.1"
                     /translation="MRVLYFSAHQIQAYFWRGGECTLEDTFDCEAPGAHFDAYLKTHR
                     RSLFAVVANVADEGFHHETIPFLQSRDRAVVVSRRLNQLFQNSPLSVAVSLGYEKSRR
                     KDEKLALLGLTNAALFDPAIKAMRAAEVKVAGIYSLPLLASAILERLKVTTTRCLLVT
                     VQDNTIRQTYFDNGNLVISRLAPLGDSSAIGVTHAVGTEVARFQQYLLSQRTITRNDS
                     LDVHIVLHQDLAQSAVSTLQGSEQLQF"
     gene            complement(748..1947)
                     /locus_tag="VIS73_03490"
     CDS             complement(748..1947)
                     /locus_tag="VIS73_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5762249.1"
                     /translation="MALFDYKAVAPDGRMVFGRIDALNMIDLEMRLKRMELDFVSGKP
                     IKTHGLLGGKIPRRELINFCFHLEQLTRAGVPILEGLADLRDSIENPRFREVVASLIE
                     SIEGGATLSQAMGSHRKVFDKVFVSLVRAGEATGRLSEVLRSVVESLKWEDELASQTK
                     KVVMYPAFVGTVVIGAAVFLMVYMVPQLKQFVKNMGQELPAQTKALFFISDMLVAYWY
                     IFAALPIIAVISTFVLFRINPIFRRSFDSLKLRLPVAGPILRKIILSRFANTFALLYS
                     SGIPILDSIRTTQDVVGNRVIRAGLRRVEELIGEGQSVTAAFHAVGMFPPLVVRMLKV
                     GENTGGLDVALSNVGYFYNRDVKESVEKLQAMIEPALTVFLGAILGWIMLAVLGPVYD
                     VISKIKA"
     gene            complement(1952..>3046)
                     /locus_tag="VIS73_03495"
     CDS             complement(1952..>3046)
                     /locus_tag="VIS73_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY5762250.1"
                     /translation="EPEQNFLRVRYRIDGILRQIRSLHKSYWPAMAVRIKVMSGMNIA
                     ESRAPQDGRINLTISGRQIDFRVSAQPTIHGENIVLRILDRQKGIVPLEALGLADDHL
                     DLLKVMIARPEGIILVTGPTGSGKTTTLYSILNHLNADSVNIMTLEDPVEYPMAMVRQ
                     TSIAETTKLDFADGIRSIMRQDPDIILVGEVRDNETAQMAFRASMTGHQVFSTLHTNS
                     AIGAIPRLLDIGILPDIMAGNIIGVIAQRLIRKLCNHCKRPYVAEPLELRLLGHVLEH
                     ERPTLYRPTGCEKCEFQGYKGRVAVTELLRMTEEIDEAVARKATAREIRSLAHQQGFR
                     SLADDALRRVLAGATSIEEVGRVVDLTDRM"
BASE COUNT          596 a          888 c          972 g          590 t
ORIGIN      
        1 tggaactgca gttgctcgct gccttgcagc gtcgacacgg ctgactgcgc caggtcctga
       61 tggagcacga tatgaacatc gagcgagtcg ttgcgcgtga tcgttcgttg gctcagcaaa
      121 tattgctgga atcgcgcgac ctcggtgcca accgcatggg tgacgccaat ggcactgctg
      181 tcgccaaggg gcgccagccg gctgatgacc aggttgccgt tgtcgaagta ggtctgccga
      241 atcgtattgt cctgaaccgt gaccagcaga caacgcgttg tggtcacctt caggcgctca
      301 agtatcgcgc tcgccagcag cggcagcgag tagatcccgg cgaccttgac ctcggcggcc
      361 cgcatcgcct tgatcgccgg atcaaacagc gccgcgttgg tcaggccgag cagggcgagt
      421 ttctcgtcct tgcgccgcga cttttcgtag cccagggaga ccgcgaccga cagcggcgag
      481 ttctggaaca actggttcag gcggcgacta accaccacgg cgcggtcacg cgattggagg
      541 aagggaatcg tttcgtgatg aaagccctcg tcggcgacgt tggcgacaac ggcaaacagg
      601 ctgcgccggt gggttttcag gtaggcgtcg aaatgagcgc cgggcgcttc gcagtcgaag
      661 gtgtcttcga gcgtgcactc cccaccgcgc caaaagtacg cctggatttg gtgggcagag
      721 aagtagagaa cgcgcatcgc gggcatatca ggccttgatc ttgctgatta cgtcatagac
      781 cggaccgaga accgccagca tgatccagcc gagaatggcg ccgagaaaga cggtgagtgc
      841 tggttcgatc atcgcctgca gcttttccac cgattccttc acgtcgcgat tgtagaaata
      901 accgacgttc gaaagcgcga cgtcgaggcc gcccgtgttt tcgccaacct tgagcatgcg
      961 aaccaccagc ggcgggaaca tgccgacagc gtggaaggcg gcagtcacac tctggccttc
     1021 gccgatcagt tcttcgacgc ggcgcagccc ggcccggatg acccggttgc cgacgacgtc
     1081 ctgtgtcgtg cggatcgaat cgagaatcgg aattcccgat gagtagagca gggcgaaggt
     1141 attggcaaag cgtgacagga tgatcttgcg caggattgga ccggcaaccg gtagtcgcag
     1201 tttcaggctg tcgaagctgc gccggaagat cggattgatc cgaaacagca cgaaggtgga
     1261 aatcaccgcg atgatcggca gcgccgcaaa aatgtaccag taggcaacca gcatgtcgga
     1321 gatgaaaaac agcgccttgg tctgggccgg gagttcctgg cccatgttct tcacgaactg
     1381 cttgagctgc gggaccatgt agaccatcag aaagaccgcg gcgccaatga cgacggtgcc
     1441 gacgaaggcc ggatacatga cgaccttctt ggtttgcgac gcgagttcgt cttcccactt
     1501 gagcgactcg accacgctgc gcaacacttc cgacaagcgg ccggtggcct cgccggcccg
     1561 caccaggctg acgaagacct tgtcgaagac tttccggtgt gagcccattg cctgagacag
     1621 cgtcgcgcca ccttcaatcg attcgatgag gctggcgacg acttcgcgaa agcgtgggtt
     1681 ctcgatcgaa tcccgcaggt cggcaaggcc ttcgaggatt ggcacgccag cgcgggtcag
     1741 ctgctcgagg tgaaaacaga agttgatcag ttcacggcgt ggaatctttc cgccaagcag
     1801 gccatgggtc ttgatcggtt tgccgctgac gaagtcgagc tccatgcgct tcaggcgcat
     1861 ttcgagatcg atcatgttca gcgcgtcgat ccggccgaag accatgcgtc cgtcaggggc
     1921 gactgccttg tagtcgaaga gcgccatgtc tttacatccg gtcggtcaga tcaacgacgc
     1981 gaccgacttc ctcgattgac gttgcaccgg cgagcacgcg acggagcgcg tcgtcggcca
     2041 gcgaacggaa accctgctgg tgggcgagcg agcggatctc acgcgcggtg gccttgcgcg
     2101 cgactgcctc gtcgatctcc tcggtcatgc gtaacagttc ggtcaccgcg acccgaccct
     2161 tgtaaccctg gaactcgcac ttctcgcagc cggtcggccg atagagcgtt ggccgttcgt
     2221 gttcaagcac gtgcccgagc agccgcagct caagcggctc ggcaacgtag ggacgcttgc
     2281 agtggttgca gagcttgcgg atcagccgct gtgcaatgac gccgatgatg ttgccggcca
     2341 tgatgtcggg caggatgccg atgtccagca agcgcggaat ggcgccgatt gccgaattgg
     2401 tgtgcagcgt cgagaacact tgatggccgg tcatcgaggc gcggaaggcc atctgcgcgg
     2461 tttcgttgtc gcgcacctcg ccgaccagaa tgatgtccgg atcctgacgc atgatcgagc
     2521 ggatgccgtc ggcaaagtcg agcttggtgg tctcggcgat cgacgtctgc cgcaccatcg
     2581 ccatcgggta ttcgaccggg tcttccagcg tcatgatgtt gacgctgtcg gcgttgaggt
     2641 ggtttaggat cgaataaagc gtcgtcgtct tgccgctgcc ggtcgggccg gtgacgagga
     2701 tgatgccttc ggggcgggcg atcattacct tgagcagatc gaggtgatcg tcggcaagcc
     2761 ccagagcttc gagcggcacg atgcccttct gccggtcgag gatgcgcagg acgatatttt
     2821 cgccatggat cgtcggttgt gctgaaaccc ggaaatcaat ctgccggccg ctgatcgtca
     2881 ggttgatccg gccgtcctgc ggcgcccggc tttcagcgat attcatcccg ctcatcacct
     2941 tgatgcggac cgccatcgcc ggccaatacg atttgtgcag cgagcgaatc tggcgaagga
     3001 tgccgtcgat gcggtaacgc acccgcagga aattctgctc gggctc
//
LOCUS       DATDAA010000106         4643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_287560,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000106 DATDAA010000000
VERSION     DATDAA010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287560"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VIS73_03500"
     CDS             complement(<1..621)
                     /locus_tag="VIS73_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5762251.1"
                     /translation="MLAYIVRRLLYAMPILIGVNLMTFALFFVVNSPDDMARMQLGVK
                     RVTPEAIERWKADRGYDKPLFWNGAAAGTGKLSETIFFDKSLRMFAFDFGRAEDGRDI
                     GTEIGERMGPSLAIAVPVFLLGLWVTISLALLLVFLRATAIDFWGVVLCVAMMSISGL
                     FYIIGGQFLVSKLWNLVPISGYAAGLDAWRFLALPVLIGVVAGIGSS"
     gene            complement(641..1852)
                     /locus_tag="VIS73_03505"
     CDS             complement(641..1852)
                     /locus_tag="VIS73_03505"
                     /inference="COORDINATES: protein motif:HMM:NF025059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY5762252.1"
                     /translation="MSRNWAQGQHRLGHWLGEQDALWRFSPFDEAGLEQIKARLSPKL
                     SGILTGLREDEVLGMLAAEDEGRALRVHLAPGLPALAEIQQTLDEMIPGLRRAATEPA
                     TLHMMHARRGIGGRKLEQIDAFLDAVGPLRMPALDWCAGKGHLGRLAALRDDVAVDCL
                     ERDPALCRSGAQLARRDAVTLRFIAADALSPVAEPQLAGRHVLALHACGELHRRLVDH
                     ADRLGVRALDVAPCCHHRGNDLADLAADAATPFAPAPSQLRLSVTGYATASASQRQRH
                     RRLLGWQCALRRVSRQCWGLPSDSLPSQQAGAAEACRFSDWAAPAARKQGMSLPAAPE
                     LAAHEAAGHAEALDMLRASLLRLACQRPIETWLVARLAARLEGQGYAVTVRRFCAAPL
                     TPRNLLVSARH"
     gene            complement(1849..3999)
                     /locus_tag="VIS73_03510"
     CDS             complement(1849..3999)
                     /locus_tag="VIS73_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5762253.1"
                     /translation="MRPVLGRLLFVAVAGLSAGCGEVWNDPYPAAEAGQNILYSAFTE
                     RPKHLDPAVAYAEDQYTFIAQIYEPPLQYHYLKRPYELIPATAQSVPVPRYFDAAGRE
                     LPAGAPPAKVARSVYEIRIQPGILYQPHPAFPDSDPVSMSVPPVVPGFPQQGTRELVA
                     ADYVYGIKRLAHPALHSPIFGFMANYIVGLGELGKALEKDYFARPEAARGEWLDLDQY
                     PLTGVEIVDRYRYRITLKGKYPQFLYWLAMPFFAPVPREADRFYAQPGMAEQNLTLDW
                     YPVGTGPYYLAENNPNSRMALARNPHFRGEPYPCIGEDEDEAAGLLTDCGKTMPFIDR
                     VVFSREKETIPYWNKFLQGYYDASGISSDSFDQAVRIGDGGEVALSEEMRARGISLLT
                     AVRASTFYMGFNMLDPAVGGLTEAGRKLRQAISIAIDQEEFIAIFMNGRGQTAMSPLP
                     PGLFGFADGRIGVNPVTHVWQEGPGGGRAVRRSVAEAKRLLAEAGYPNGRNAKTGEPL
                     VLNLDTTSAGLGDKPRLDWLTRQFAKIDIQLVVRSTDFNRFQEKLRKGAVQIFYLGWN
                     ADYPDPENFYFLLYGPEARTLSQGENSANYQSAEFDRLFSKMKHMDNTPDRLAIIREM
                     TALLQKDAPWVFGFHPKSYGLGHGWLYNRKPNNVANNTLKYQRLDPEKRRVARTEWNR
                     PVRWPLWLVAGLILLLIAPVYGHWRRRERQAVGE"
     gene            4149..>4643
                     /locus_tag="VIS73_03515"
     CDS             4149..>4643
                     /locus_tag="VIS73_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5762254.1"
                     /translation="MGFLAGKRILITGLLSKHSIAYGIAKACHREGAQLAFTYQTERF
                     ADRLAKFAGEFDSKLMFPCDVADDAQIEALFASLGKEWDGLDGLVHSIAYAPTEAIEG
                     DFLEAISRDAFRIAHDISSYSFAGMAKAARPMLLKGNKASVLCLSYLGAVRTMPNYNV
                     MGLAK"
BASE COUNT          839 a         1533 c         1513 g          758 t
ORIGIN      
        1 gctgctaccg attccggcaa cgacgccgat cagcaccggc aatgccagga agcgccaggc
       61 atcgagcccc gcggcataac ccgagatcgg caccaggttc cacagcttgc tgacgagaaa
      121 ctgcccgccg atgatgtaga agagacccga gatcgacatc atcgcgacgc agagcacgac
      181 gccccagaaa tcgatcgccg tcgcgcgcag gaaaaccagc agcagcgcca ggctgatggt
      241 cacccacaga ccgagcagga acaccggcac ggcaatcgcc agcgatggcc ccatgcgctc
      301 gccgatctcg gtgccgatgt cgcggccgtc ctcggcacgg ccgaagtcga aggcgaacat
      361 acgcagcgac ttgtcgaaga agatcgtctc ggagagcttg ccggttcccg ccgcggcgcc
      421 gttccagaac agcggcttgt catagccgcg atcggccttc cagcgttcga tcgcctccgg
      481 tgtcacgcgc ttgacgccga gctgcatgcg cgccatgtcg tcgggcgagt tgacgacgaa
      541 gaacaacgcg aaggtcatca ggttcacgcc gatcaggatc ggcatcgcgt agagcaggcg
      601 gcgaacgatg taggcgagca tggcgggcga ctatatcgtg tcagtgacgg gccgagacca
      661 gcaggttgcg cggcgtcagc ggcgccgcgc agaagcgccg cacggtaacc gcgtaaccct
      721 gcccctcgag ccgcgcagcc aagcgggcga ccaaccaggt ctcgatcggc cgctggcagg
      781 cgagccgcaa cagcgacgcg cgcagcatgt ccagcgcctc ggcatgcccg gcggcctcat
      841 gtgccgccaa ttcgggcgct gctggcaacg acatcccttg tttccgggca gccggtgccg
      901 cccaatcgga gaaacgacac gcctcggccg cacccgcctg ttgcgacggc aacgagtcgg
      961 aaggcagccc ccagcactgt cgtgacaccc gccgcaaggc gcactgccag ccgagcaggc
     1021 gccggtgtcg ctggcgctgg ctggccgatg ccgtcgcgta gccggtaacc gacagacgca
     1081 gctgcgatgg cgccggcgcg aacggtgtcg cagcgtccgc cgcgagatcg gcgaggtcgt
     1141 tgccgcgatg gtggcaacag ggtgcgacat cgagcgcgcg cacgcccagg cggtcggcgt
     1201 ggtcgaccaa gcgccggtgc agttcgccgc aggcatgcag cgcgagcacg tggcggccgg
     1261 cgagctgcgg ctcggccacc ggcgacagcg catcggcagc gatgaaacgc agcgttaccg
     1321 catcgcgccg cgccaattgc gcgccgcttc gacacagcgc cggatcgcgt tcgaggcaat
     1381 cgacggcgac atcgtcgcga agcgccgcaa gccgcccaag gtgccccttg ccagcgcacc
     1441 agtcgagcgc cggcatgcgc agcgggccca ccgcatcgag aaaggcatcg atctgttcga
     1501 gcttgcggcc accgatgcca cggcgcgcgt gcatcatgtg tagcgtcgct ggctccgtgg
     1561 ccgcgcgccg gagccccgga atcatctcgt cgagcgtctg ctgaatctcc gccagtgccg
     1621 gcagaccagg cgccagatgc acgcgcagcg ccctgccctc atcctcggcg gcgagcatcc
     1681 ccagcacctc gtcttcgcgc aggccggtga ggatccctga gagcttcggc gacagccgcg
     1741 ccttgatctg ctcaaggccg gcttcatcga agggggagaa ccgccagagc gcatcctgct
     1801 cgccgagcca gtgcccgagc cgatgctgcc cttgtgccca gttgcggctc attcgcccac
     1861 tgcctgtcgc tcacgccggc gccagtggcc gtagaccggc gcgatcaaca acagaatcag
     1921 cccggccacc agccacagcg gccagcgcac cggccgattc cattcggtac gggcgacccg
     1981 gcgcttctcc gggtcgagac gctggtattt caacgtgttg ttggcaacgt tgttcggctt
     2041 gcggttgtag agccagccat ggccgagccc gtagctcttc ggatgaaagc cgaacaccca
     2101 gggcgcatcc ttctgcagga gcgccgtcat ctcacggatg atcgccagtc gatcaggcgt
     2161 gttgtccatg tgcttcatct tcgagaacag gcgatcgaac tcggcgctct ggtagttcgc
     2221 cgagttctcg ccctggctga gcgtgcgcgc ttccgggccg tacagcagga aatagaaatt
     2281 ctcggggtcc gggtagtcgg cattccagcc caggtaaaaa atttgcacgg cgcccttgcg
     2341 cagcttctcc tgaaagcggt tgaagtcggt gctgcgcacg acgagttgga tgtcgatttt
     2401 ggcgaactgc cgcgtcagcc agtcgaggcg cggcttgtcg ccgagtccgg ccgaggtggt
     2461 atcgagattg agcaccagcg gctcgccggt cttggcatta cggccattgg ggtaaccggc
     2521 ttcggcgagc aggcgcttcg cttcggcgac actgcggcgc accgcacggc cgccgcccgg
     2581 cccctcctgc cagacgtggg tgaccggatt gacgccgatg cgcccgtcgg caaaaccaaa
     2641 caggcccggc ggcaacggac tcatcgccgt ctgcccgcgg ccgttcatga agatcgcgat
     2701 gaactcttcc tggtcgatgg cgatcgagat cgcctgccgc agctttcgcc cggcctcggt
     2761 gagaccgccg accgccgggt cgagcatgtt gaagcccatg tagaaagtcg aggcgcgaac
     2821 cgcggtcagc aggctaatgc cgcgtgcgcg catctcctcg ctcagcgcca cctcgccgcc
     2881 gtcgccgatg cgcactgcct ggtcgaagct gtccgagcta attcccgagg catcgtaata
     2941 gccctggaga aacttgttcc agtaggggat cgtttccttc tcgcgcgaaa aaaccacgcg
     3001 gtcgatgaac ggcatggttt tcccgcagtc ggtgagcagg cctgccgcct catcctcgtc
     3061 ctcaccgatg cacgggtacg gttcgccgcg gaaatgcgga ttacgcgcca gtgccatgcg
     3121 gctattggga ttgttctcgg cgaggtagta cggcccggtg ccaaccgggt accagtcgag
     3181 cgtgaggttc tgctcggcca tccccggctg cgcgtagaag cgatcggcct cgcgcggcac
     3241 cggcgcgaag aacggcatcg ccagccaata gagaaactgt gggtacttgc ccttgagcgt
     3301 gatgcgatag cggtagcgat cgacgatctc gacgccggtc agcgggtact gatcgagatc
     3361 gagccactcg ccgcgcgccg cttcgggccg tgcgaagtaa tccttctcga gcgccttgcc
     3421 gagttctccc aggccgacga tgtagttcgc catgaagcca aagatcggcg aatgcaacgc
     3481 cggatgcgcg aggcgtttga tgccgtagac gtaatcggca gcgaccagct cgcgcgttcc
     3541 ctgttgcgga aaacccggaa ccaccggcgg taccgacatc gacaccgggt cgctgtctgg
     3601 aaatgccggg tgcggctggt agaggatgcc cggctggatg cggatctcgt agacgctgcg
     3661 ggccacctta gccggcggcg caccggccgg cagttcacgc ccggccgcat cgaagtagcg
     3721 gggaaccgga actgactgcg cggtcgccgg aatcaactcg tagggccgct tcaggtagtg
     3781 gtactgcagc ggcggctcgt aaatctgcgc gatgaaggtg tattggtcct cagcgtaggc
     3841 aacggccggg tcgaggtgtt tgggacgttc ggtaaaggcc gagtagagga tgttctggcc
     3901 ggcctctgcg gccggatagg gatcgttcca gacctcgccg cagcccgcgg acagtccggc
     3961 aacggcgacg aacaggagcc ggccaagaac gggtcgcatg cgcatgcggc gagtgtagcg
     4021 aatggaccgc gcccgtgcca tcgcccggcg aaaaacccgc attccgcggc ttgcgcatgt
     4081 gctgcgccgc ggcagcattg gctataatcc gcgctctgca tcgcgctaga acttagggag
     4141 gcgccaagat gggttttctt gccggcaagc ggatactcat taccggactg ttgtcgaagc
     4201 actcgattgc ctatggcatt gccaaggcct gtcaccgtga gggtgcgcaa ctcgccttca
     4261 cctaccagac cgagcgtttc gccgaccggc tcgcgaaatt cgcgggtgaa ttcgacagca
     4321 aactgatgtt cccctgcgat gtcgccgacg acgcgcagat cgaggcgctg ttcgcttcgc
     4381 tcggcaagga atgggacggc ctcgacggct tggtccattc gatcgcctac gcgccgaccg
     4441 aggcgatcga gggcgacttt ctcgaagcga tcagccgcga tgccttccgc atcgcccacg
     4501 acatctcctc gtacagcttc gccggcatgg ccaaggcggc gcggccaatg ctgctcaagg
     4561 gcaacaaggc gtcggtgctc tgcctttcct atcttggcgc cgtgcgcacg atgcctaact
     4621 acaacgtgat gggtctggcc aag
//
LOCUS       DATDAA010000107         3068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_462102,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000107 DATDAA010000000
VERSION     DATDAA010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462102"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..2261)
                     /locus_tag="VIS73_03520"
     CDS             complement(24..2261)
                     /locus_tag="VIS73_03520"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY5762255.1"
                     /translation="MASGKSKIIYTLTDEAPLLATCAFLPIIRTFTAPAGIEVEKADI
                     SVSARVLAEFSDLLPPEQRVPNTLAELGKKTLQPDANIIKLPNISASVGQLMTCIKEL
                     QSKGYALPAYPEDPKTEAEKDILARYSRCIGSSVNPVLREGNSDRRAPAAVKNYVRKH
                     PHSMGEWKQWSQSHVSHMHSGDFYHGEKSMTLDKARDVRMELTNKAGKTTVLKQKVAL
                     QDGEVIDSMFMSKKALCDFYERELDDCREAGILFSLHVKATMMKVSHPIVFGHCVKIY
                     YKDAFEKHGKLFDEIGVNVNNGMANLYEKIAALPESKRDEIVRDLHACQEHRPRLAMV
                     DSAKGITNFHSPNDIIVDASMPAMIRGGGKMWGADGKQYDAKAVMPESTFARIYQEMI
                     NFCKWHGNFDPKTMGTVPNVGLMAQKAEEYGSHDKTFEIAEDGIANIVDIATGEVLLT
                     QNVEMGDIWRMCQVKDAPIRDWVKLAVTRARNSNTPAIFWLDPYRPHENELIKKVQKY
                     LKDHDTKGLDIQIMSQVRAMRFTLERVARGLDTISVTGNILRDYLTDLFPIMELGPSA
                     KMLSIVPLMAGGGMYETGAGGSAPKHVQQLVQENHLRWDSLGEFLALAVSLEELGIKE
                     NNARAKLLAKTLDAATGKLLDNNKSPSPKTGELDNRGSQFYLALYWAQALAAQTEDKE
                     LAGHFAKLAKSLAENEAKIVDEMKAVQGKAVDIGGYYKADSEKTKAVMRPSATFNAAL
                     KAARA"
     gene            2523..>3068
                     /locus_tag="VIS73_03525"
     CDS             2523..>3068
                     /locus_tag="VIS73_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase kinase/phosphatase AceK
                     regulatory subunit"
                     /protein_id="HEY5762256.1"
                     /translation="MTRSPNATAQQMAVALLEGFDKHYRLFRATSAEAKQRFENADWG
                     RVQAAVRDRIQFYDDRVNEAVERLNTEFNATAVDDETWQQAKLQYIGMLLNHKQPELA
                     ETFFNSVTCKILHRTYFHNDFIFVRPAVATEYIESDPPTYRSYYPPAGALRDTFRRIV
                     LDFGWKTPFADLERDVGYVYRR"
BASE COUNT          515 a          966 c          947 g          640 t
ORIGIN      
        1 cggcgatgcc ggtcagcgcc cgctcaggcc cgtgccgcct tcagcgcagc gttgaaggtc
       61 gcgctcggcc gcatcacggc tttggtcttc tccgagtcgg ccttgtagta gccgccgatg
      121 tcgacggcct tgccctgcac cgccttcatc tcgtcgacga tcttcgcctc gttctcggcc
      181 agcgacttgg cgagcttggc gaagtggcct gccagctcct tgtcctcggt ctgcgccgcg
      241 agcgcctgcg cccagtacag cgccaggtag aactgcgagc cgcggttgtc gagctcgccg
      301 gtcttcggcg acggtgactt gttgttgtcg agcagcttgc cggtggcggc atcgagcgtc
      361 ttcgccagca gcttggcgcg ggcgttgttc tccttgatgc ctagttcctc gagcgagact
      421 gctagcgcga ggaattcacc gagcgaatcc cagcgcagat ggttttcctg caccagttgc
      481 tgcacgtgct tgggcgccga accaccggca ccggtttcgt acatgccgcc accggccatc
      541 agcggcacga tcgacagcat cttggcggac ggaccgagtt ccatgatcgg gaacaggtcg
      601 gtcaggtagt cgcgcaggat gttgccggtc accgagatgg tgtcgagccc gcgtgcgaca
      661 cgctcgagcg tgaagcgcat ggcgcgcacc tgcgacatga tctggatgtc gaggcccttg
      721 gtgtcgtgat ccttaaggta tttctggacc ttcttgatca gctcgttctc gtgcggacga
      781 taggggtcga gccagaagat cgccggcgta ttggagttgc gcgcgcgggt gaccgcgagc
      841 ttgacccagt cgcgaatcgg cgcgtccttg acctggcaca tgcgccagat gtcgcccatc
      901 tcgacgttct gcgtcagcag cacttcaccg gtagcgatgt cgacgatgtt ggcaatgccg
      961 tcttcggcaa tctcgaaggt cttgtcgtgc gagccgtatt cctcggcttt ctgcgccatc
     1021 aggccgacgt tgggcacggt gcccatggtc ttcggatcga agttgccgtg ccatttgcag
     1081 aagttgatca tctcctgata aatgcgggcg aaggtcgatt cgggcatgac cgccttggcg
     1141 tcgtactgct taccgtcggc gccccacatc ttgccgccgc cgcgaatcat cgccggcatc
     1201 gaggcgtcga cgatgatgtc gttgggcgag tggaagttgg tgatgccctt ggccgaatcg
     1261 accatggcca gccgcggacg gtgttcctgg caggcatgca ggtcacggac gatttcgtcg
     1321 cgcttcgatt cgggcagcgc ggcgatcttc tcgtagagat tggccatgcc gttgttgacg
     1381 ttgacgccga tctcatcgaa cagcttgccg tgcttttcga aagcatcctt gtagtagatc
     1441 ttgacgcagt gaccgaagac gatcgggtgc gagaccttca tcatcgtcgc cttgacgtgc
     1501 agcgagaata gaatcccggc ttcgcggcaa tcatcgagct cgcgctcgta gaagtcgcac
     1561 aacgccttct tgctcatgaa catcgaatcg atgacctcgc cgtcctgcag cgccaccttc
     1621 tgcttgagca cggtggtctt gccggccttg ttggtcagct ccatgcgcac gtcgcgcgcc
     1681 ttgtcgagcg tcatcgactt ttcgccgtgg tagaagtcgc cgctgtgcat atgcgagaca
     1741 tggctttgcg accactgctt ccactcgccc atcgaatgcg gatgcttgcg cacgtagttc
     1801 ttgaccgcgg cgggcgcgcg gcggtcggag ttgccttcgc gcaggaccgg gttcaccgaa
     1861 ctgccgatgc agcgcgagta gcgcgcgagg atgtccttct cggcttcggt cttcgggtct
     1921 tcggggtagg cgggcaaggc gtagcccttg ctctgcaatt ccttgatgca ggtcatcagc
     1981 tggccgaccg aggcgctgat gttgggcagc ttgatgatgt tggcgtcggg ctgaagcgtc
     2041 ttcttgccga gttcggcgag cgtgttcggc acgcgctgct cgggcggcag caggtcggaa
     2101 aattcggcga gcactcgagc cgaaacggag atgtccgcct tttcgacttc aatgccagcc
     2161 ggcgccgtga aagtgcggat gattggcagg aacgcgcagg tcgccagcag cggcgcctcg
     2221 tcggttagcg tgtagatgat ctttgatttg ccagatgcca tgctctctcc gttgggcaac
     2281 aagtaggtgg gtcggaacgt cgcgaaggcc gcgattttcc ctcattccgc ttaactcagg
     2341 ctgacggcct gcaaatcagg cccgctgcgc tgcccgcgac ggccggcgaa atccggctgc
     2401 aagccgcgcc agtgctagaa tttgcggccc cgtcggcggg cagcgcagga tcggcaccag
     2461 caacaacaat accgcccgcg actgccggca gaacggcccg catcgctttc cgaggattcg
     2521 gcatgaccag atcgcctaac gctaccgcgc agcagatggc cgtcgcgctg ctggaaggtt
     2581 tcgacaagca ctaccggctg tttcgcgcta ccagcgccga agccaagcag cgcttcgaga
     2641 acgccgactg gggtcgcgtc caagccgccg tccgcgaccg catccagttc tacgacgacc
     2701 gcgtcaacga agccgtcgag cgcctcaaca ccgaattcaa cgccaccgcg gtcgacgatg
     2761 agacctggca gcaggccaag ctccaataca tcggcatgct gctcaaccac aagcagcctg
     2821 agttggccga gaccttcttc aactcggtga cctgcaagat cctgcaccgg acctatttcc
     2881 acaacgactt catcttcgtt cgcccggcgg tggcgaccga gtacatcgag tccgacccgc
     2941 cgacctaccg cagctactat ccgccggccg gtgccctgcg cgacaccttt cgtcgcatcg
     3001 tcctcgattt cggctggaaa acgccattcg ccgacctcga acgcgatgtc ggctatgttt
     3061 atcgccgc
//
LOCUS       DATDAA010000108         2014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_322256,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000108 DATDAA010000000
VERSION     DATDAA010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322256"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(324..1559)
                     /locus_tag="VIS73_03530"
     CDS             complement(324..1559)
                     /locus_tag="VIS73_03530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5762257.1"
                     /translation="MPWLIQFCLARVAFATMFTVWSAVLPLLKDDWALSAWQAGMVQS
                     AYHVGFLVSLFSVGFLSDRFGARRVYLLSSVAAVASAMLFAFLADGFASALLLYGLTG
                     LCSGGSYTPGLALVAQQVTQRQGRAMGLYLAAASLGYALGLLISSAMLPLAGWRGAFV
                     VTACGPVIGMALGYWSLRGVPNVVHPRPEGHGNRAAIGEVLRNKPAMLSIWGYTFHAW
                     ELLGLWAWLPAFLAMAAVAGGGDAANAVSLGATLSALTYLAAVVGSVAGGTLSDRWGR
                     TTVILIMSLTSLACSFGFGWMIGMPLWLLVAVAVIYNVSGVADSSIHSTTLTELTPPR
                     YLGAAYSLRSVLGFGAGAIAPWCFGLVLDAGRAIELDTSTVWGLAWATLGVGGLLGPL
                     ATWRLRLNPAAEKLAGGRR"
     gene            complement(1701..>2014)
                     /locus_tag="VIS73_03535"
     CDS             complement(1701..>2014)
                     /locus_tag="VIS73_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762258.1"
                     /translation="PRGPVIFPVPWARTPEASFLATPDELKQLLHRAGFDIAGWVDST
                     AAGRDWFVAVAGKMRERGLPPVGIHLLLGPEFMEMAQNQARNLQEGRIALAQIVARKV
                     A"
BASE COUNT          389 a          709 c          645 g          271 t
ORIGIN      
        1 gagtgaccta catttcacat ccggcaagcc gggacggtct ctctttcgca aggggtatgc
       61 cagctcggtt tgatcggctc gtaacggcct tcctgcaagt cttttgggcg ctttcaaccg
      121 gcgcaaacta cctgtcgcga acacgacaat gtccgccgcc gggcggccgg gcccgaacaa
      181 agtaaagcga gtggcggcgg ttcaggcagt ctcggcgagc tgctcggagc ggcgtcgcag
      241 ctcagcatcg ccaagctgtt gcgcgtttgt tgcgctccaa tgattgactg gctgcaataa
      301 tcgcgaactg atccgtgacg tcctcagcgt ctccccccgg caagcttctc cgccgcggga
      361 ttcagccgca gacgccaggt ggccaagggc ccgagcagtc cgccaacgcc cagcgtcgcc
      421 caggccagcc cccagacggt agacgtatcg agctcgattg cccggccggc atcgagcacc
      481 aggccgaagc accacggcgc aattgccccg gcgccgaagc ccagcaccga gcgcagcgag
      541 taagcagccc ccaggtagcg cggcggcgtg agttcggtca acgtggtcga gtggatcgac
      601 gaatcggcga cgccgctgac gttgtagatc accgcaaccg ccaccagcag ccacaacggc
      661 atgccgatca tccagccgaa gccgaacgag caggccaggc tggtcagcga catgatcagg
      721 attaccgtag tgcgccccca gcggtcggac agcgtgccgc cggcgacgct accgacgaca
      781 gcagccaaat aggtcagcgc gctcagcgtt gcaccgaggc tcaccgcgtt ggcggcatcg
      841 cccccgcctg caaccgcggc catcgccagg aaggccggca gccaggccca caggccgagc
      901 agttcccagg catggaaggt gtagccccaa atcgacagca tcgccggctt gttgcgcagg
      961 acctcgccga tcgccgcgcg gttgccgtga ccctcgggtc gcgggtgcac gacattggga
     1021 acgccgcgca gcgaccaata gccgagcgcc atgccgatga ccgggccgca ggcggtgacg
     1081 acgaaggcgc cgcgccagcc ggccagcggc agcatggcgc tggaaatcag caagcccagc
     1141 gcgtagccca gcgaggcggc tgccaggtac agccccatcg cccgcccctg ccgttgcgtc
     1201 acctgctggg ccacgagggc gaggcccggg gtgtaggagc cgccggaaca caggcccgtc
     1261 aggccgtaca gcagcagggc cgaggcaaag ccatcggcga gaaaggcgaa cagcatggcg
     1321 ctggcgaccg cagccacgct gctcaacaga tagacgcgcc gcgcgccgaa gcgatcggac
     1381 aggaagccca ccgaaaacag cgagacgaga aatcccacgt gataggccga ctgcaccatg
     1441 cccgcctgcc aggcgctgag cgcccagtcg tccttgagca gcggcaggac cgccgaccag
     1501 acggtgaaca tcgtcgcgaa agccacgcgc gccagacaga actggatcag ccacggcatg
     1561 tttcgactcc ggctgcctgt cccgcaaaca gtttgagcgc attggcgatc atcgctacgc
     1621 gccccgaggt gtcacgatgc cgcacctgtg gtcggcggcg ccatcgcgac gtcgcgtgtt
     1681 gaagaggcga cggcgatgag tcacgcaacc ttcctggcaa cgatctgcgc cagcgcgatt
     1741 cgcccttcct ggaggttgcg cgcctggttc tgggccatct ccatgaactc cggccccagc
     1801 agcaggtgaa tccccaccgg cggcaatccc cgctcgcgca tcttcccggc gacggcgacg
     1861 aaccagtccc ggcccgctgc agtactgtcg acccatccgg cgatgtcgaa cccggcacgg
     1921 tgcagcaatt gcttcagctc gtccggcgtg gcgaggaagc tcgcctccgg cgtccgtgcc
     1981 cacggcaccg gaaagatcac cgggccgcgc ggac
//
LOCUS       DATDAA010000109        12277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_322365,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000109 DATDAA010000000
VERSION     DATDAA010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12277
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322365"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VIS73_03540"
     CDS             <1..712
                     /locus_tag="VIS73_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762259.1"
                     /translation="PGSERDFEPGMAAVEPPPAPLTPAQRKHRLELMAFRKSFADFPA
                     GAPRSRVAIDDLKRGDVLRIRTIVGNVFLRIVDRIREHGSHAGEILCECHYDLGGQCN
                     PAHAASVTLPICSKDQVITGADGTQRQASRKLKIRSETELPPQLGKLLDERFFLELVI
                     YGNPQAEKLRPVDVWRAVARILLKAARLVWRGLLKIKALIDENNRREREKKEQKAAAQ
                     RQRQEAKKAAREARRKPD"
     gene            complement(761..1489)
                     /locus_tag="VIS73_03545"
     CDS             complement(761..1489)
                     /locus_tag="VIS73_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5762260.1"
                     /translation="MSHRRVAIVSGASRGLGLESARQLAERGFELVMLARGKQALQAA
                     AAEIGNATGIEPMAVAVDVTDAVAVRALVDAVEQRYGRADVLINNAGVFLGPKDFASP
                     AAGSVLSASPDEVAETLAVNVIGPLHMVQAFVPMMRVQRWGRIVNVSSGMGQLAEMGG
                     AWPGYRISKTALNALTRILSVELQGSGIKVNSVCPGWCRTDMGGDDASRSAVDGAKSI
                     VWAALLDDDGPSGGFFRDGKPLDW"
     gene            1651..2661
                     /locus_tag="VIS73_03550"
     CDS             1651..2661
                     /locus_tag="VIS73_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HEY5762261.1"
                     /translation="MSLTRPHLICPAGSLPALKAAVDNGADAVYLGFKNDTNARNFAG
                     LNFDDKTLKEGIRYAQSKGREVLIAINTYAQPGRVAEWRRSIDTAAELGADAVILADA
                     GLLEYAHRTHREIRLHLSVQGSATSYEAINFAHEMFGVRRAVLPRVLTLAQVEHVIRN
                     TPVEIEVFGFGSLCVMNEGRCWLSSYATGESPNTVGACSPAKYVKWEQKPGSMEVRLN
                     DILIDRFADGEPAGYPTICKGRFEVGGETYYAMEEPVSLNVLEILPDILKIGVAAIKV
                     EGRQRSPTYVAQVTRTLRAALDAALAAPDRYAVKSAWQAELAKVSEGTQATLGAYNRP
                     WR"
     gene            2679..3584
                     /locus_tag="VIS73_03555"
     CDS             2679..3584
                     /locus_tag="VIS73_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEY5762262.1"
                     /translation="MKLTLGPLLWFWPKAEVLDFYAQVSECAAIERVYVGEVVCSRRQ
                     QLRTADWLGLARDLAGAGKEVVLAAQALLESESDLKALRRLIDDSRFAVEANDLGAVK
                     VARDAGVPWVAGPHLNVYNEAALAFFADKGAIRWVPPLEATKTLVETLHRERPTGTET
                     ELFAFGRLPLAFSARCFTARHYGLNKDDCQFKCMDHPEGMTVSTRENRAFLTLNGIQT
                     MSAQTYSLLERVPEMQALGIEALRISPQPEHTLAIVDAFRRAVAGEAVAHDPAWASDG
                     LCDGFWQGKAGIFCDAGNYAESAAA"
     gene            3619..4086
                     /locus_tag="VIS73_03560"
     CDS             3619..4086
                     /locus_tag="VIS73_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY5762263.1"
                     /translation="MNTLTIPTFRLPALVARLGGRLPQWPHAVGIAGALNAARLMRLL
                     PIESLDALEGKLFRVAVNDTGSVAHFEYERSSFRPVFAPSRTPDLSFSADLSAYLKLL
                     ARQEDPDTLFFNRQLMIEGNTELGLMVKNMLDAVEWNRIPGYALFERLGSGHA"
     gene            complement(4133..4996)
                     /locus_tag="VIS73_03565"
     CDS             complement(4133..4996)
                     /locus_tag="VIS73_03565"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY5762264.1"
                     /translation="MSSYRYQIARSFLRQKNAEHIERYPQLACFAYDAITLAIHMDGR
                     YERDELEFLAERIFPALPQPAACLDIGANIGNHSLHFARHFDRVIAFEPHPRTFGLLA
                     INAAMAANVVALNCGASRQAGTVSVNESRTNMGASAIGRSGGDGGNTVSFALQRIDEV
                     PEVTACERIAFVKIDVEGHEREALEGAAETLRRHRPVIALEALGDEVEDGTTTALALL
                     REIGYAHIYELADQGLIGRLPRRLAKAARALVALLTGRRVRRPRRLQRVERLEKRNYP
                     MLLCAMEPING"
     gene            complement(5113..6939)
                     /gene="phaC"
                     /locus_tag="VIS73_03570"
     CDS             complement(5113..6939)
                     /gene="phaC"
                     /locus_tag="VIS73_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEY5762265.1"
                     /translation="MSQPQDPQAPMPNPADVATAYAEVAQRASRLLTQVIEKKTKAGV
                     APPADELGIARAFMDLSSRLLSNPYQLAQSQMNMMWDYFSLWQHSTLKMMGMPLTPIA
                     APEKADKRFKDEEWEQHFLFDFMKQSYLIAARHIHDTVSNVEGLDSHTQDKVNFFTRQ
                     YIDALSPSNFALTNPEVFRETVKSHGQNLVKGFSNLLEDIEKGDGQLRISMTDTTAFE
                     MGKNIATTPGKVVFENELFQLIHYVPSTEKQHKRPLLIVPPWINKYYILDLREKNSFV
                     KWATDQGYSTFIMSWVNPDGDLAHKTFEDYVLEGSMVAVDVVEKITGEKEIDTAGYCL
                     GGSLLATTLAYMAAKKDKRITSATFFTTMIDFSFPGELGVFIDEEAIQGLEKRMAEKG
                     YLEGSEMAGTFNMMRANDLIWSFVVNNYLMGKDPFPFDLLYWNSDSTRMPFAMHSFYL
                     RNMYMNNKLKDPGGITIGGVPIDMRKIKTPSYFISTIEDHIAPWQSTYLGAKLFSGPV
                     KFVLGGSGHIAGIVNPPVANKYGYWINEKLPESSDDFLTGATQHAGSWWNDWSAWVQK
                     LGDGTMVEPRVPGKAKGFKVIEDAPGRYAKLRLDTKKKPAEA"
     gene            complement(6979..8010)
                     /locus_tag="VIS73_03575"
     CDS             complement(6979..8010)
                     /locus_tag="VIS73_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5762266.1"
                     /translation="MDATGNVKLIGEIPPAGSWAEIAPQVRWLRMPLPFALDHINLWL
                     LEAADGFTIVDTGFALPEVQAAWETVLVGLEKPVRRILVTHFHPDHLGLAAWLVERTG
                     AELLMTTGEYLTGWAVWSQAAGHGVPDMLRFFRSHGLDEARCAALDGRGNAYRRGVPA
                     IPGQYCRLHDGDRLRLTDAEWVVQVGHGHAPEHAALYCAEANVLISGDMLLPRITTNI
                     SVFAVTPEADSLREYLSSLAYWQDLSAEALVLPSHGRPFHGAAQRIAELGAHHDARLA
                     DVLAACAGAPMSAAEMLPVLFDRELDAHQTMFAMGEAIAHLNYLEHAGWINRQLGDDG
                     VLRYRRSQQ"
     gene            8113..9285
                     /locus_tag="VIS73_03580"
     CDS             8113..9285
                     /locus_tag="VIS73_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEY5762267.1"
                     /translation="MDLRGLDFGLGETIEMLRDTVRAFAEKEIAPRAAEIDRINEFPA
                     DLWRKLGEIGLHGMTVSEEYGGSDMGYLAHIVALEEISRASASVGLSYGAHSNLCVNQ
                     IHRNGSEAQRQRYLPKLISGEHVGALAMSEPNAGSDVVSMKLRADKKGDRYVLNGAKM
                     WITNGGDADTLIVYAKTDLTAGPKGITAFIVEKGTKGFSHGSHLDKLGMRGSNTYPLF
                     FDDCEVHEENVIGGVGNGVKVLMSGLDYERAVLCGGPLGIMAACMDVVVPYLHERKQF
                     DTPIGEFQLMQGKLADLYTTWKACQAYVYALGRACDQSDHARSLRKDAAGAILYAAEK
                     ATWMAGEAIQVLGGNGYINEYPTGRLWRDAKLYEIGAGTSEIRRMLIGRELFNETS"
     gene            9331..9675
                     /locus_tag="VIS73_03585"
     CDS             9331..9675
                     /locus_tag="VIS73_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY5762268.1"
                     /translation="MNTQPPTLGRAIGPLSLPPQENRAGIAGLANLGPASARMLRAAG
                     IDNVAQLNEFGAVEAFRRVRASGAAPSLNLLWALEGALTGRHWQEVAHAERTSLLLEL
                     DALDALEKRKRT"
     gene            9672..10862
                     /locus_tag="VIS73_03590"
     CDS             9672..10862
                     /locus_tag="VIS73_03590"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY5762269.1"
                     /translation="MTDPVVIVAAARTPLGGLQGCFASQSAANLGAVAIRTAVERAGI
                     SADLIDEVLFGCVLQAGQGQAPARQAALQAGLPFRAGCTTVHKVCGSAMKATMLGHDL
                     LLAGSQSVVVAGGMESMSNAPYLLPKARAGYRLGHGQVLDHMFLDGLEDSYTAEHRGR
                     LMGTFAEDCAASFRFTRGQQDEFAIASLARAKAANSDGSFAWEVAPVTVTTRKGDTVV
                     DRDEQPFTANIEKIPSLKPAFRKDGTVTPANSSSISDGAAALVLMRASMAEKLGSQPI
                     ARIVAHTTHAHEPALFPTAPVGAMQKLLDRIGWQAKDVDLFEINEAFAVVAMAAMHEL
                     SLPHDKVNIHGGACALGHPIGASGARIIVTLLGALKKIGGRRGVASLCIGGGEATALA
                     VEMV"
     gene            10999..11907
                     /locus_tag="VIS73_03595"
     CDS             10999..11907
                     /locus_tag="VIS73_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY5762270.1"
                     /translation="MSTAQAMHLRLVGVAFFWGGTWVAARIAVADLPPLTVAAWRFLL
                     AVLVLGGVLMLRGGLPRFTLRQWIIVSALGATGIFFYNLCFLYGIRLIEAGRGALVVA
                     LTPVVIAAADALLFGARMTPRKALGVALAMIGCLSIVTRGDLLALFAGEVGLGEWLIL
                     GCVLMWTTYTFIGRRATVGLTPLAATFGACLTGGAMLTVASLIEGSYTQVADAGIGAL
                     ASIAFLGILGTALAFTWYADGVKQVGATRAGIYINLVPVFAVLQGALLLGERLGLPSL
                     IGGLLVLIGVLLTTVEPAALRKELPA"
     gene            11904..>12277
                     /locus_tag="VIS73_03600"
     CDS             11904..>12277
                     /locus_tag="VIS73_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5762271.1"
                     /translation="MILDQDHQMIRDAVREFAQGEIAPHAAEWDRSHQFPRDALKGLA
                     ALGVLGTVVPEDLGGAGLDYLSLVLALEEIAAADGSTSTIVSVQNSLVCGIVMKYGSD
                     THRRDLLPALARGEKLGCFCLT"
BASE COUNT         2139 a         4115 c         3923 g         2100 t
ORIGIN      
        1 gcccggcagc gaacgcgact tcgagcccgg catggcagcg gtcgagccgc ccccggcgcc
       61 gctgacgccg gcgcagcgaa agcaccgcct cgaactcatg gcgttccgca agtccttcgc
      121 cgactttccg gctggcgcgc cgcgctcgcg ggtcgccatc gacgacctca aacgcggcga
      181 cgtgctgcga atcaggacga tcgtcggcaa cgtcttcctg cgcattgtcg accgcatccg
      241 cgaacatggt tcacacgccg gcgagatcct ctgcgagtgc cactacgacc tcggcgggca
      301 gtgcaatccc gcccacgccg cgagcgtcac gctgccgatc tgctccaagg atcaagtcat
      361 tacgggcgcc gacggcacgc agcggcaggc cagtcgcaag ctgaagatac gtagcgaaac
      421 agagctgccg ccgcagctag gcaagctgct tgacgagcgc ttttttctcg agctggttat
      481 ctacggcaac ccgcaggcgg aaaagctgcg gccggtcgat gtctggcgtg cggtcgcccg
      541 cattctgctg aaggccgcac gcctggtttg gcgcgggctg cttaagatca aggcattgat
      601 cgacgagaac aaccgtcggg agcgcgagaa aaaggagcag aaggccgcgg cgcaaaggca
      661 aaggcaggag gcaaagaagg ccgcccgcga agcccgccgg aaacccgact gaacccagcc
      721 ggcgcagcgc ccgccacgtc gacccgcgca ccgcaccggc ctaccagtcg agcggcttgc
      781 cgtcgcgaaa aaagccgccg ctcggcccgt cgtcatcgag cagcgccgcc cagacgatgg
      841 acttggcgcc gtcgactgcg gaacggctgg catcatcgcc gcccatatcg gttcggcacc
      901 agccggggca aacgctgttc accttgatgc cactgccctg cagctcgact gataggattc
      961 gcgtcaaggc gttgagcgca gtcttgctga tccggtagcc gggccaggca ccgcccatct
     1021 cggcgagctg gcccattccc gacgagacgt tgacgatgcg cccccagcgc tgcacccgca
     1081 tcatcggcac gaaggcctgc accatgtgca gcggaccgat gacgttcacc gccagcgttt
     1141 cggcgacctc gtcgggactg gcgctcagca cgctgccggc tgcggggctg gcgaaatcct
     1201 tgggcccgag aaagacgccg gcgttgttga tcagtacgtc ggcgcgtccg taacgctgtt
     1261 cgaccgcatc gacaagtgcg cggacggcaa cggcatcggt gacgtcgacg gcgacagcca
     1321 tcggctcgat gccggtggcg ttgccgattt cggcggcggc agcctgcagt gcttgcttgc
     1381 cgcgcgccag catgaccagc tcaaagcccc gctcggctag ctggcgggcg ctttccagtc
     1441 cgagtccgcg gctcgcgccg ctcacgattg ctacgcgtcg gtgtgacatt cgcatcactc
     1501 cccgtttgca cccgaatggg cgtggaaaaa ccctgccgaa tcaatggcaa agaatcggac
     1561 ggcgggcaac ccgagtataa ttcgcggctt tcgcgaacgc gacccgaatc taacgggccg
     1621 cggtatgccc gacaccgcca ggagcgcgcc gtgagtctca cccgccccca cctgatctgc
     1681 cccgccggca gcctgccggc gctgaaggcc gccgtcgata acggcgccga cgccgtttac
     1741 ctcggcttca aaaacgacac caatgcccgc aacttcgccg gcctaaactt cgacgacaag
     1801 acgctcaagg aaggcatccg ctacgcccag agcaagggtc gtgaagtcct gatcgcgatc
     1861 aatacctacg cccaacctgg gcgggtcgcc gaatggcgcc gctcgatcga caccgccgcc
     1921 gagctggggg ccgacgcggt gattctcgcc gacgccggcc tgctcgaata cgcgcatcgc
     1981 acgcaccgcg agatccgcct ccacctctcg gttcagggtt cggcgaccag ctacgaggcg
     2041 atcaacttcg cgcacgagat gttcggcgtg cgccgcgccg tgctgccgcg cgtgttgacg
     2101 ctggcgcagg tcgagcacgt gatccgcaat acgccggtcg agatcgaggt cttcggcttc
     2161 ggtagcctgt gcgtgatgaa cgaagggcgc tgctggctct cctcctacgc caccggcgag
     2221 tcgccaaaca cggtcggcgc ctgctcgccg gcaaagtacg tgaagtggga gcagaagccc
     2281 ggttcgatgg aggtccgcct caacgacatc ctcattgacc gcttcgccga cggcgagccg
     2341 gcgggttatc cgacgatctg caagggtcgc ttcgaggtcg gcggcgagac ctactacgcg
     2401 atggaagaac cggtcagcct caacgtgctc gagatcctcc cggacatcct caagatcggt
     2461 gtcgcggcga tcaaggtcga agggcggcag cggagcccga cctatgttgc gcaggtgact
     2521 cgcacgttgc gtgccgcgct cgatgccgcc ctcgccgcgc ccgaccgcta cgccgtgaag
     2581 tcggcctggc aggccgaact ggcgaaggta tccgaaggca cgcaggcaac gctcggcgcc
     2641 tacaaccggc cctggcgctg ataagcggat cgaccgttat gaaacttacc ctcggacctc
     2701 tcctctggtt ctggcccaaa gccgaagtcc ttgatttcta cgcgcaggtt tccgaatgcg
     2761 cggccatcga acgtgtctac gttggcgaag tcgtctgttc ccgccgccag cagttgcgca
     2821 cggccgactg gctcgggttg gcgcgcgatc tcgctggcgc cggcaaggaa gtggtactcg
     2881 cggcgcaggc gctgctcgaa tcggaaagcg acctcaaggc gttgcggcgg ctgatcgacg
     2941 attctcgctt tgcggtcgag gccaatgatc tcggcgccgt caaggtcgcc cgcgatgccg
     3001 gcgtgccctg ggttgccggc ccgcatctca acgtctacaa cgaggcagcg ctggcctttt
     3061 ttgctgacaa gggcgcgatc cgctgggtgc cgccgctgga ggcgaccaag acgctggtcg
     3121 agaccctgca ccgggagcgg ccgaccggca ccgaaaccga gctgttcgcc ttcggccgcc
     3181 tgccgctggc cttctcggcc cgctgcttca cggcgcgcca ctacgggctg aacaaggacg
     3241 actgccagtt caagtgcatg gaccaccccg agggcatgac cgtatcgact cgcgagaacc
     3301 gcgccttcct gacgcttaac ggcatccaga cgatgtcggc acagacctat tcgctgctcg
     3361 aacgcgttcc cgaaatgcag gcgctcggca tcgaggcgct gcgcatcagc ccgcagcccg
     3421 agcacacgct ggcgatcgtc gatgccttcc gccgggcggt cgccggtgaa gcggtcgcgc
     3481 acgacccggc ctgggccagc gacggccttt gcgatggctt ctggcagggc aaggccggta
     3541 tcttctgcga cgcgggcaac tacgccgagt cggcggcagc ctgacccggc cgccgccaac
     3601 gatcaacgga gtcaatagat gaacacactg accatcccca cctttcgcct gccggcgctg
     3661 gtcgcccgcc ttggcggccg actgccgcag tggccgcacg cggtcggcat cgccggtgcg
     3721 ctgaacgcag cacggctgat gcgcctgctg ccgatcgaat ccctcgatgc actcgagggc
     3781 aagctgttcc gcgtggccgt caacgacacc ggcagcgtcg cccacttcga atacgaacgc
     3841 agcagcttcc gcccggtctt cgcgccgtcg cgcacacccg acctgtcctt ttccgccgac
     3901 ctctccgcct acctcaagct actggcgcgg caggaagacc cggacacgct gttcttcaat
     3961 cggcagctga tgatcgaggg caacaccgaa ctcggactga tggtgaagaa catgctcgac
     4021 gccgtcgagt ggaaccgcat tcccggttac gcactgttcg aacggctcgg tagcgggcac
     4081 gcctgaagca gcacgccgcc ggcgatcgca gctctaggcc ccgccgcgac gctcagccgt
     4141 tgatcggctc catggcgcag agcaacatcg gatagttgcg cttctccagc cgttcgacgc
     4201 gctgcagacg ccgcggccgt cgtacgcgcc ggccggtgag caaggccacc agcgcccgcg
     4261 ccgccttcgc cagccgccgc ggaagccgtc cgatcaatcc ctgatcggcc aattcgtaga
     4321 tgtgcgcgta gccgatctcg cgcagcaagg ctagcgccgt cgtcgtgccg tcttcgacct
     4381 cgtcgcctag cgcctccagc gcgatcaccg gccggtggcg tcgcagcgtc tcggccgcgc
     4441 cttcgagcgc ctcgcgctcg tgtccttcga cgtcgatctt gacgaaggct attcgctcgc
     4501 aggcagtcac ctcgggcacc tcgtcgatcc gctgcagcgc aaaggaaacg gtattaccgc
     4561 catcaccgcc ggagcggccg atggccgacg cgcccatgtt ggtccggctt tcgttgaccg
     4621 atacggttcc ggcctgcctc gacgctccgc agtttagcgc caccacgttg gcggccatcg
     4681 cggcgttgat cgccagcaag ccaaaggtcc gcgggtgggg ctcaaaggcg atcacccggt
     4741 cgaaatgccg ggcgaagtgc aggctatggt tgccgatgtt ggcgccaatg tcgaggcagg
     4801 cagccggctg cggcagcgcc gggaagatcc gctcggcaag gaactctagc tcgtcgcgct
     4861 cgtagcggcc atccatgtgg atcgccagcg tgatcgcgtc gtaggcaaag cacgcgagtt
     4921 gcggatagcg ctcgatatgt tcggcgtttt tctggcgcag aaagctgcgg gcaatctggt
     4981 agcggtagct gctcattcgt atctctcact cggtccgggc acggcgccgc ccgaatgctg
     5041 ccgcccacgc gcgggcgacc ggaaaaaata cggcgccacc cggcgccgta ttcactgcga
     5101 ccaagccggt cgtcaggctt cggctggctt tttcttggta tcgagacgca gcttggcgta
     5161 acggcccggc gcgtcttcga tgaccttgaa gcccttagcc ttgcccggta cgcgcggctc
     5221 gaccatggtc ccgtcgccga gtttctgcac ccaggcgctc cagtcgttcc accacgaccc
     5281 ggcgtgctgc gttgccccgg tgaggaagtc gtccgaggac tcgggcagct tctcgttgat
     5341 ccagtagcca tacttgttgg cgaccggcgg attgacgatg cccgcaatgt ggcccgatcc
     5401 gccgagcacg aacttgaccg gaccgctgaa cagcttggcg ccgaggtaag tgctctgcca
     5461 tggggcgata tggtcttcga tggtcgagat gaagtaggac ggcgtcttga tcttgcgcat
     5521 gtcgatcggc acgccgccga tcgtgatgcc gcccggatcc ttcagcttgt tgttcatgta
     5581 catgttgcgc aggtagaagc tgtgcatcgc gaagggcatg cgcgtcgaat ccgagttcca
     5641 gtagagcagg tcgaagggga acggatcctt gcccatcagg tagttgttca ccacgaagga
     5701 ccagatcagg tcgttggccc gcatcatgtt gaaagtgccg gccatttccg aaccctcgag
     5761 gtagcccttc tcggccatgc gcttctcaag cccctgaatc gcttcctcgt caatgaaaac
     5821 gccgagttcg cccgggaagg agaagtcgat catcgtggtg aagaaggtcg ccgaggtaat
     5881 gcgcttgtct ttcttcgcgg ccatgtaggc gagggtcgtc gccagcaggc tgccacccag
     5941 gcagtagcct gccgtgtcga tttccttctc gccggtgatc ttctcgacga catcgacggc
     6001 aaccatcgaa ccttcgagta catagtcctc gaaggtcttg tgcgccagat cgccgtccgg
     6061 gttcacccag ctcatgatga aggtgctgta accctgatcg gtcgcccatt tgacgaagga
     6121 gttcttctcg cgcaggtcga ggatgtagta cttgttgatc cacggcggaa cgatcagtag
     6181 cgggcgcttg tgctgcttct cggtactcgg gacatagtgg atcagctgga acagctcgtt
     6241 ctcaaagacc accttgcccg gcgtcgtggc gatgttcttg cccatctcga aggcagtagt
     6301 gtcggtcatc gagatgcgca gctggccgtc acccttttcg atgtcctcga gcaggttgct
     6361 gaaacccttg accaggttct ggccgtggct cttgacggtc tcgcggaaga cctcggggtt
     6421 ggtcagcgcg aagttggacg gcgacagcgc gtcgatgtac tggcgcgtaa agaaattcac
     6481 cttgtcctgg gtgtggctgt cgaggccctc gacgttgctt accgtgtcgt ggatgtgacg
     6541 ggccgcgatc aggtagctct gcttcatgaa gtcaaacagg aagtgctgtt cccactcctc
     6601 gtccttgaag cgcttgtcgg ccttctcggg cgcggcaatc ggcgtcagcg gcatgcccat
     6661 catcttcagc gtcgagtgct gccacagcga gaagtagtcc cacatcatat tcatctgcga
     6721 ctgcgccagc tggtacggat ttgaaagcag ccgcgaggaa aggtccatga aggcgcgggc
     6781 gatgccaagc tcgtcggcgg gcggcgcgac gcccgccttg gtcttcttct cgattacctg
     6841 cgtcagcaag cgcgaggcgc gctgagcgac ttcggcgtag gcggtggcga catcggccgg
     6901 attcggcatc ggcgcctgcg ggtcctgggg ctgcgacata aagctctcct tttatatttt
     6961 cgatcctgct gcgggggctt actgctgact gcgtcggtag cggagcacac cgtcgtcgcc
     7021 aagctggcga ttgatccatc cggcatgctc caggtaattc aggtgggcga ttgcctcgcc
     7081 cattgcaaac atcgtctggt gcgcatcgag ctcccggtcg aacaacaccg gcagcatctc
     7141 tgctgccgac atcggcgccc cggcacaggc ggccagtaca tcggcgagcc gcgcgtcgtg
     7201 gtgcgcgccg agttcggcga tccgctgcgc cgcgccgtga aacggccgtc cgtgcgaggg
     7261 caagaccagc gcctcggcag acaggtcctg ccagtatgcg agcgaactca ggtattcccg
     7321 caaggaatcg gcctcgggcg taaccgcgaa cacgctgatg ttggtggtga tccgcggcaa
     7381 cagcatgtcg cctgaaatta gcacgttggc ttcggcgcag taaagggccg cgtgctccgg
     7441 ggcgtgcccg tggccgacct gaacaaccca ttcagcgtcg gtcagccgca gccggtcgcc
     7501 gtcatgaagc cggcaatact ggccggggat ggccggcacg ccacgccggt aggcgttgcc
     7561 gcgtccgtcg agcgcggcgc agcgcgcctc gtcgaggccg tggctgcgga agaagcgcag
     7621 catgtcggga acgccatgcc ccgcggcctg cgaccagact gcccagccgg tcaggtactc
     7681 accggtggtc atcagcagtt cggcgccggt ccgctcgaca agccaggccg ccaggccgag
     7741 gtgatcgggg tggaaatgcg tgacgaggat gcggcgcacc ggcttttcca gacccaccag
     7801 cacggtctcc caggctgcct gcacctcggg caacgcgaag ccggtatcga cgatggtgaa
     7861 accatcggcg gcttcgagca gccagagatt gatgtggtcg agcgcaaagg gtagcggcat
     7921 gcgcagccaa cgcacctgtg gcgcgatctc ggcccaactg ccggccgggg ggatttcccc
     7981 gatcagcttg acgtttccgg tggcgtccat tcaaataggt cttccatacg gtaccgtttg
     8041 gaactctaac acacacaaag ccttgattca aaggcggatt ccagtttatt tcagcactgg
     8101 acggaggaga cgatggactt gagaggactc gatttcgggc tcggcgaaac gattgagatg
     8161 ctgcgcgata ccgtgcgtgc cttcgccgaa aaggaaatcg ccccgcgtgc cgccgagatc
     8221 gaccggatca acgaattccc ggccgacctg tggcggaagc tcggcgaaat aggcttgcac
     8281 ggcatgaccg tctccgagga atacggcggc agtgacatgg gttatctggc ccacatcgtt
     8341 gcgctcgagg agatttcccg cgcctcagct tcggtcggac tatcctacgg cgcccattcg
     8401 aacctctgcg taaaccagat ccaccgcaac ggcagcgagg cgcagcgaca gcgctacctg
     8461 ccgaaactga tctccggcga gcacgtcggc gcactggcga tgagcgagcc gaacgccggc
     8521 tccgatgtgg tcagcatgaa gctgcgagcg gacaagaagg gcgaccgcta cgtactcaac
     8581 ggcgcaaaga tgtggatcac caacggcggc gacgccgaca cgctgatcgt ctacgcgaag
     8641 accgacctga ccgctggacc gaagggaatc accgccttca tcgtcgaaaa gggcaccaag
     8701 gggttttcgc acggcagcca cctcgacaag ctaggcatgc gcggctcgaa cacctacccg
     8761 ctgttcttcg acgactgcga agtccatgag gaaaacgtca tcggcggtgt cggcaatggc
     8821 gtcaaggtgc tgatgagcgg gctcgattac gagcgcgccg tgctctgcgg cgggccgctc
     8881 ggcatcatgg cagcgtgcat ggacgtggtc gttccctacc tgcacgagcg caagcagttc
     8941 gatacgccga tcggcgaatt ccagctgatg cagggcaagc tcgccgacct gtacaccacc
     9001 tggaaggcct gccaggccta tgtctatgca cttggccgcg cctgcgacca aagcgatcac
     9061 gcacgcagcc tgcgcaagga cgccgccggc gcgattctct acgccgccga aaaggcgacc
     9121 tggatggccg gcgaagcgat ccaggtcctc ggcggcaacg gctacatcaa tgagtacccg
     9181 accggtcgcc tgtggcgtga tgccaaactc tatgaaatag gcgccggcac gtcggagatc
     9241 cggcgcatgc tgatcggccg cgagctgttc aacgagacgt cctgagcgcc agacttggca
     9301 gcgtgaattg cacagaccca ggagcgcaac atgaatacgc aaccgccgac gctggggcgg
     9361 gcaatcggcc ccctcagtct gcccccccag gagaaccggg ccggcatcgc cggactcgcc
     9421 aacctaggcc cggcatcggc acgcatgctg cgcgcagccg gcatcgacaa cgtcgcccaa
     9481 ctgaatgagt tcggcgctgt cgaggctttt cgccgggttc gcgccagcgg cgccgcgccc
     9541 agcctgaacc tgctgtgggc attggagggc gctctgaccg ggcgacactg gcaggaagtg
     9601 gcgcacgccg agcgaacctc gctactgctt gaactcgatg cgctcgacgc gctggaaaag
     9661 aggaaacgca catgaccgat cccgtcgtca tcgtcgccgc ggcgcgcacg ccgctcggcg
     9721 gcctgcaggg ctgctttgcg tcgcaaagcg ccgccaacct cggcgcagtg gccatccgca
     9781 ccgccgtcga acgcgccggc atcagcgccg acctgatcga cgaagtgcta ttcggctgcg
     9841 tgttgcaagc cggccagggt caggcgccgg ccagacaggc ggcgttgcag gctgggctcc
     9901 cgtttcgcgc cggctgcacc accgtgcaca aggtctgcgg ctcggcaatg aaggcgacca
     9961 tgctcggcca cgacctgctg ctcgccggca gccagtcggt agtggtcgcc ggcggcatgg
    10021 agtcgatgag caacgccccc tatctgctgc cgaaggcgcg cgccggctat cggctcggcc
    10081 acgggcaggt gctcgaccac atgtttctcg acggccttga ggattcgtac accgccgagc
    10141 accgcggccg gctgatgggc acctttgccg aggattgcgc ggcgagcttc cgcttcaccc
    10201 gggggcagca ggacgagttc gccatcgcct cgctcgcccg cgccaaggcg gcgaacagcg
    10261 atggcagctt cgcctgggaa gtcgcgccgg tgacggtaac gacgcgcaag ggcgacacgg
    10321 tcgtcgaccg cgacgagcag ccattcaccg ccaacatcga gaaaatcccg tcgctcaagc
    10381 cggccttccg caaggacggc acggtgaccc cggccaactc ctcgtcgatt tccgacggcg
    10441 cggcggcact ggtcctgatg cgcgcgtcga tggccgagaa gctcggcagc caaccgatcg
    10501 cgcggatcgt cgcccacacc acgcacgcgc acgaaccggc gctgttcccg acagcaccgg
    10561 tcggcgccat gcagaaactg ctcgaccgca tcggctggca ggcgaaggac gtcgacctct
    10621 tcgagatcaa cgaggccttc gccgtggtgg cgatggcggc gatgcacgaa ctgtcgctac
    10681 cgcatgacaa ggtcaacatt cacggcggcg cctgcgcgct cggccatccg atcggcgcct
    10741 ctggtgcacg catcatcgtc actctgctcg gtgcgctgaa gaagatcggc ggccgccgcg
    10801 gcgtcgccag cctctgtatc ggcggcggcg aagccactgc ccttgcggtc gagatggtgt
    10861 gagccaggca agcccccacc ggccgcaccc gaaggcggcc acgctagtct cccccttcca
    10921 cgggaagagg gttgggggga aggccgcctg cccacgcggg aaacggtccg ggggaatgtg
    10981 gcgtgaatgg atgccggcat gagcaccgcc caggccatgc acctgcgcct cgtcggcgtc
    11041 gctttcttct ggggcggcac ctgggtcgcc gcgcgcattg ccgtcgccga cctaccaccg
    11101 ctgaccgtcg ccgcctggcg cttcctgctc gccgtgcttg tgctcggcgg tgtgctgatg
    11161 ctgcgcggtg gcctgccgcg ctttacgctg cggcaatgga tcatcgtcag cgcgctcggc
    11221 gccaccggaa tcttttttta caacctctgc ttcctttacg gcatccgcct gatcgaggct
    11281 ggccgcggcg cgctggtggt ggcgctgacg ccggtggtga tcgccgccgc cgacgcgctg
    11341 ctgttcggcg cgcggatgac gccgcgcaag gcactcggcg tcgccctggc gatgatcggc
    11401 tgcctgagca tcgtcacccg cggcgacctg ctggcgcttt ttgccggcga agtcgggctc
    11461 ggcgaatggc tgattctcgg ctgcgtgctg atgtggacga cctacacctt catcggtcgc
    11521 cgcgcgaccg tcggcctcac gccactcgcc gcgaccttcg gcgcctgcct gaccggcggc
    11581 gcaatgctga ccgtcgcctc gctgatcgaa ggcagctaca cgcaggtcgc cgatgccggg
    11641 atcggcgcgc tggcgtcgat cgccttcctc ggcatcctcg gcaccgcgct cgcctttact
    11701 tggtacgccg acggcgtcaa acaggtcggc gccactcgcg ccggcatcta cataaacctc
    11761 gtgccagtct tcgccgtact gcagggcgcg ctgctgctcg gcgagcgcct tggcctgccc
    11821 tcgctgatcg gcggcctgct ggtgctgatt ggcgtactgc tgacgaccgt ggagcccgcc
    11881 gcattacgta aggagttacc cgcatgatcc tcgaccagga ccaccagatg attcgcgacg
    11941 cggtgcgtga gttcgcccag ggcgagatcg cgccgcacgc ggccgaatgg gaccgcagcc
    12001 accagtttcc gcgcgacgcg ctgaagggtc tcgccgcgct cggcgtactc ggcaccgtcg
    12061 tacccgaaga cctcggcggt gccggcctcg actatctgtc actggtgctc gcgcttgaag
    12121 agatcgccgc tgccgacggc tcgacctcga ccatcgtcag cgtccagaac tcgctggtct
    12181 gcggcatcgt catgaaatac ggcagcgaca cgcaccggcg cgacctgctg ccggcactgg
    12241 cacgcggcga gaaactcggc tgcttctgcc taaccga
//
LOCUS       DATDAA010000110         2634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_392516,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000110 DATDAA010000000
VERSION     DATDAA010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392516"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..164)
                     /locus_tag="VIS73_03605"
     tRNA            complement(89..164)
                     /locus_tag="VIS73_03605"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(129..131),aa:Ala,seq:cgc)
     gene            complement(255..1244)
                     /locus_tag="VIS73_03610"
     CDS             complement(255..1244)
                     /locus_tag="VIS73_03610"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY5762272.1"
                     /translation="MSESIAAIERAAGLLAAGELVAFPTETVYGLGADASNSAAVARV
                     FAAKGRPADHPLIVHLPGHDALNAWAVEIPDLAWELAEHFWPGPLTMILRRAPGVADA
                     VTGGQDTVGLRVPGHPLALDLLRAFARIKDGAASGIAAPSANRFGRISPTRAIHVQAE
                     LGDHVACVLDGGACPVGIESTIIDLSRGRPVILRPGQLAVETLSMVAGCDIGIEESAK
                     APRVSGTLESHYAPVTPLILVESTRLAATLRDLRGQGKRAAVLAYGVSPGMSGMAKRW
                     LPAAPNATTYAHDLYANLRELDAAGADLIVVETVPDETGWLAVRDRLARAASR"
     gene            complement(1241..2386)
                     /locus_tag="VIS73_03615"
     CDS             complement(1241..2386)
                     /locus_tag="VIS73_03615"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY5762273.1"
                     /translation="MILPPATLGMLGGGQLGRFFVAAAHELGYRVWVLDPDPHSPAGR
                     IADRHLQADYVDQDALLALAENCAAVSTEFENVPAVALEQLAKFVPVRPGAAAVAVCQ
                     NRIAEKNFLREHGIPHAPFAVIDAETDFAALPNALFPGILKVARNGYDGKGQAQVDGV
                     ESLRDAWRGFHQEACVLEAKLSLDCEISVVLARRANGEVACFPAAENRHRNGILDVSI
                     VPAGVASSLRTEAIEIAQRVARELDYVGVLAVEFFVCGNRLHVNEMAPRPHNSGHYTI
                     DACITSQFEQQVRVLCGLPLGDPAAHSSAVMVNLLGDLWYAKAAAHPAEPPWASIFDT
                     PGLKLHLYGKHHARAGRKMGHFTVLGDPGAALVDAALAARTAIGVGT"
     gene            complement(2475..>2634)
                     /locus_tag="VIS73_03620"
     CDS             complement(2475..>2634)
                     /locus_tag="VIS73_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY5762274.1"
                     /translation="TFAIGEAGAANAALFAVAMLATADAGLQAKLLAFRQAQEERVLA
                     MKLPDAGA"
BASE COUNT          448 a          872 c          904 g          410 t
ORIGIN      
        1 tctttgcagt tcgcgacttg aacgacatcg ttgcaatcgc tacaacttta tccttccggt
       61 caaaatcgct gcaagccctt gttttaattg gtggagagga aggggatcga accctcgacc
      121 tccgcattgc gaacgcggcg ctctcccagc tgagctaccc ccccacacga acggcgcgga
      181 ttatagcaag gcgcggcagc ctgtcaaaaa gggcgattgc gggaagtggc gttctgtagc
      241 agcgggtggc ggcgtcaacg cgacgccgcc cgcgccagcc ggtcgcgaac cgccagccag
      301 ccggtttcgt cgggcacagt ctcgacgacg atcaggtcgg cgcccgcggc gtcgagttcg
      361 cgcagattgg cgtagagatc gtgcgcgtag gtcgtcgcat tcggcgctgc cggcagccag
      421 cgcttggcca ttcccgacat gcccggcgag acgccatacg ccagcacggc ggcgcgcttg
      481 ccctgcccgc gaaggtcgcg cagcgtcgcg gcgaggcgcg tcgactcgac caggatcagc
      541 ggcgtcaccg gcgcgtagtg tgattcgagc gttcccgaca cccgcggcgc cttggcgctt
      601 tcctcgatgc cgatatcgca gccggcaacc atggacagcg tttccacggc gagttgcccc
      661 ggtctcagta tgaccggccg cccgcgcgag aggtcgatga tcgttgattc gatgccgacc
      721 gggcaggcgc cgccatcgag cacgcaggca acgtgatcgc cgagttcggc ctgcacgtgg
      781 atggcccggg tcggactgat gcggccgaag cgattggccg atggcgcggc gatgccgcta
      841 gcggcaccgt ccttgatccg cgcgaaggcg cgcagcaggt cgagcgccag cggatgaccg
      901 gggacgcgca ggcccacggt gtcctgcccg ccggtcaccg catcggcaac gcccggtgcg
      961 cggcgcagga tcatcgtcag cgggccgggc cagaaatgct cggccaactc ccaagcgaga
     1021 tcgggaatct cgaccgccca cgcattgagc gcatcgtggc cgggcagatg cacgatcagc
     1081 gggtgatcgg cgggccggcc cttggcggcg aagacccggg caacggcggc cgaattgctg
     1141 gcgtcggcgc cgagtccgta aacagtttcg gtgggaaagg cgacgagttc gccggctgcc
     1201 agcaggccgg cggcgcgttc gatggcggcg atcgattcac tcatgtcccg actccgattg
     1261 cggtgcgcgc cgccagtgcc gcatcgacaa gggccgcgcc cggatcgccg agcacggtga
     1321 aatgacccat cttgcgcccg gcccgggcgt gatgcttgcc gtataggtgt agcttgagcc
     1381 ccggcgtgtc gaagatcgaa gcccagggcg gctcggccgg gtgcgcggca gccttcgcgt
     1441 accacaggtc gccaagcagg ttcaccatca ccgcgctgga gtgcgcggcg gggtcgccca
     1501 gcggcaggcc gcagagtacg cgcacctgct gctcgaactg gctggtgatg caggcgtcga
     1561 tcgtgtagtg cccgctgttg tgcggccgcg gcgccatttc gttgacgtgc agacggttac
     1621 cgcagacgaa gaactcgacg gccaatacac cgacgtaatc gagttcgcgc gcgacccgct
     1681 gggcgatctc gatcgcctca gtacgaaggc tggaggcgac gccggccgga acgatggaga
     1741 catcgagaat gccgttgcgg tgacggttct cggccgccgg gaaacaggcg acctcgccgt
     1801 tcgcgcgccg cgccagcacg accgatatct cgcaatcgag cgagagcttc gcctcgagca
     1861 cgcaggcttc ctgatgaaac ccgcgccaag cgtcgcgcaa ggactcgacg ccgtcgacct
     1921 gcgcctgccc cttgccgtcg tagccatttc tcgcgacctt gaggattccc ggaaacaaag
     1981 cgttcggcag cgccgcgaag tcggtctccg cgtcgatcac cgcgaacggc gcatgcggta
     2041 tgccgtgttc gcgcaggaag ttcttctcgg cgatacggtt ctggcagacc gcaacggcgg
     2101 ccgctcccgg ccgaaccgga acgaacttgg cgagttgttc gagcgcgacc gctggcacgt
     2161 tctcgaactc ggtgctgacg gccgcgcagt tctcggcaag cgccagcagc gcgtcctggt
     2221 cgacgtagtc ggcctgcaga tgacggtcgg caattctccc ggcgggactg tgcgggtcgg
     2281 gatcgaggac ccagacccgg tagccgagtt cgtgcgcggc ggcgacgaaa aagcggccaa
     2341 gctggccgcc accgagcatg ccgagggtgg ccggcggcag gatcatcgaa agcctcctac
     2401 gcggagcggc cacgcgtggg cctgaaccgg aacggcaacg gctccgacca tgccgtgatc
     2461 gatcgccggg aagctcatgc gccggcgtcc ggcagtttca tcgccagtac gcgctcttcc
     2521 tgcgcctgac ggaaggccag gagcttcgcc tgaagaccgg catcggcagt tgccagcatc
     2581 gccacggcaa acagtgcggc gtttgcggcg ccagcctctc cgatggcgaa ggtg
//
LOCUS       DATDAA010000111        13926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_147402,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000111 DATDAA010000000
VERSION     DATDAA010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13926
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147402"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VIS73_03625"
     CDS             <1..769
                     /locus_tag="VIS73_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5762275.1"
                     /translation="VGSDAAAITTSAVRDGDHWVLNGVKQFITTGKHAEVAIVFAVTD
                     KGAGKKGISCFVIETGAPQHIPGFVVARLEDKLGQRASDTAQIVFDNCRVPASALAGA
                     EGDGYRIALSNLEAGRIGIAAQSVGMARAALEAAVRYANERETFGKPIVQHQAVAFRL
                     ADMATQLEAARQLVWHAATLKDAGQPCLKEASMAKLFASEMAERLCSDAIQIHGGYGV
                     VNDFPVERIYRDVRVCQIYEGASDIQRLVIAREVTTL"
     gene            766..1206
                     /locus_tag="VIS73_03630"
     CDS             766..1206
                     /locus_tag="VIS73_03630"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY5762276.1"
                     /translation="MTARLRTGITPEDFSRRSEGTLPGWFGVRVTAIAPGRLDAELDI
                     RDEMLAPNGYLHAAAVIALADTTAGYATIAHLPEGAENFTTVELKTNFCATQTEGRLF
                     AFATAEHAGRLTQIWDVEIIGDDDRRLALFRCTQVILWPREQKR"
     gene            1203..1805
                     /locus_tag="VIS73_03635"
     CDS             1203..1805
                     /locus_tag="VIS73_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEY5762277.1"
                     /translation="MSSPIDFYFDFSSPYGYLASEKIDELAAKHGRTVRWKPVLLGVI
                     FKTTGAAPLLNVPLKGDYAKIDIARSARSMNIPYRLPASFPQATQQAARAFYALDDAD
                     PALARRFAHAAYRAIFVDGRDISAADTVLEIAGACGADTETLAAAIATPEIKERLRRE
                     VEEAMAAGVFGSPYMMVDGEPFMGADRLPQLERWLAEGPF"
     gene            1805..2389
                     /locus_tag="VIS73_03640"
     CDS             1805..2389
                     /locus_tag="VIS73_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY5762278.1"
                     /translation="MRAITVFCGSATGKRPLYREAAEGIGRLLARRGITLVYGGGDIG
                     LMGRMAEAALQAGGEVIGVIPEALQARELGHPRLTRLEVVDSMHTRKARMAELADGFI
                     ALPGGFGTFEEFCEVLTWGQLGFHKKPCGLLDVDGYYAPLQALFGQAESEGFLQPEHR
                     RMVCIDRDAEALLEKMATYQPPDVEKWLSSVRSL"
     gene            2400..4034
                     /locus_tag="VIS73_03645"
     CDS             2400..4034
                     /locus_tag="VIS73_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY5762279.1"
                     /translation="MTVIQSTIDTRSDAYRDNVAAMQAVVDDLHAKSARIARGGSESA
                     REKHLARGKLLVRDRIDTLLDPGSPFLEIAPFAAYGMYGGNSAERGDDFEVPAASLVA
                     GIGRIAGVECMIVANDATVKGGTYYPLTVKKHLRAQEIALENRLPCVYLVDSGGAFLP
                     LQDEVFPDRDHFGRIFFNQANLSALGIPQIAAVMGSCTAGGAYVPAMSDESIIVRNQG
                     PIFLGGPPLVKAATGEDVSAEDLGGADVHTRVSGVADHYAEDDPHALAIVRRIVANLN
                     RKKSPSPTLTEPREPLYAARELYGVIPVDSKKPFDVREVIARIVDGSEFDEFKARYGT
                     TIVCGFARLWGYPVGIVANNGILFSESALKAAHFIELCAQRGIPLVFLQNITGFMVGR
                     KYENGGIARDGAKMVTAVATAKVPKFTVVIGGSFGAGNYGMCGRAYSPRFLWMWPNAR
                     ISVMGGEQAASVLATVRRDGIEAAGKTWAKDDEESFKAPIRAQYETQGHPYYASARLW
                     DDGIIDPADTRRVLGLGLSAAMNAPIEETRFGIFRM"
     gene            4042..4851
                     /locus_tag="VIS73_03650"
     CDS             4042..4851
                     /locus_tag="VIS73_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY5762280.1"
                     /translation="MSYEAIVIEIDGPVGIVTMNRAERHNAFDETLIAELTNALQALG
                     ADPAIRVVVLSSVGKSFSAGADLNWMRRAAEYGDEQNLEDARKLAALMRTLDTLPKPT
                     VARVHGPAYGGGVGLIAACDIALATFDAQFAFSEVKLGLIPAVISPYVMARIGERYAR
                     RYFLTAERFAAAEAYRIGLVHEIVPDEATLDSAVGEVVDILLKNSPQAMAAAKQLVRA
                     VSGAPIDALLIEDTTRRITEARTNPDAREGIAAFLEKRPAPWAIPAADEAQ"
     gene            4859..6895
                     /locus_tag="VIS73_03655"
     CDS             4859..6895
                     /locus_tag="VIS73_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY5762281.1"
                     /translation="MFSKILIANRGEIACRVIRTAHRMGVRCVAIHADADAGALHVRQ
                     ADEAVLIAAAEPRAAYLDGEAIIAAAHATGAQAIHPGYGFLSENADFADACAAAGLVF
                     IGPPASAIRAMGEKSAAKALMEKAGVPLTPGYHGDDQDSGLLAQQAERIGYPVLIKAS
                     SGGGGKGMRRVDAAGDFAAALASCQREAASAFGDERVLVERYLASARHIEIQVFADSQ
                     GNCVHLFERDCSVQRRHQKIIEEAPAPGLPAETRAAMGAAAVKAAQAVGYVGAGTVEF
                     IASADIARDGVFYFMEMNTRLQVEHPVTEMITGQDLVEWQLRVASGEALPLAQAQIEL
                     RGHAFEARIYAEDPERDFLPSTGTLHCLELPAPTLGARIDAGVERGDVITPHFDPMIA
                     KLIVWDGGAERPRDAALAKLRQALADTRIVGVATNIAFLSRLAANPAFVEGRVETRLI
                     DRQRESLFPPLTRTPDTVVLVATAAELLAEASHIAAQATASGDPHSPWSTPDGWRMNL
                     DATRHLLFHTGDARFEVGVAYRGDGWLLTLNGEQVPARAEALPGSRIAVELDGRRLVA
                     SVIAVNARGDAQRHVFLHGARHLLHRDDPLVQTNTQAAAGSGLNAPMPGKVVALLVEA
                     GSRVEPGTPLLVIEAMKMEHTIKAPAAGTLRAHRFAPGDPVADGELLVSFDADA"
     gene            6888..7811
                     /locus_tag="VIS73_03660"
     CDS             6888..7811
                     /locus_tag="VIS73_03660"
                     /EC_number="4.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004419 - hydroxymethylglutaryl-CoA lyase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY5762282.1"
                     /translation="MPDPLGLPTRVTLVEVGPRDGLQNEPTPVPAATKVALIGRLAAA
                     GLPVVEATSFVSAKWVPQLADAGEVLRAVLAEAAAEGTRFPVLTPNLKGFDAAIEAGA
                     REVAVFAAASESFSQKNINCSIAESLKRFEPVVASAQALDIPVRGYVSCVAGCPYEGA
                     VAPGAVATVAAALAEMGCHEVSLGDTIGTGTPASIARMLEACLRVLPAERLAGHYHDT
                     YGMAIANIHASLTLGLATFDSSVAGLGGCPYARGASGNVATEDVLYLLAGLGIETGVD
                     LDAVIAAGAFVCEALGRRNGSKVAQARLGGR"
     gene            7808..8014
                     /locus_tag="VIS73_03665"
     CDS             7808..8014
                     /locus_tag="VIS73_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEY5762283.1"
                     /translation="MIASPCVKRCAVDESGQSCTACLRTLDEIIDWPTLTDEDKRAVL
                     QRVAARRSALEAASRAALPGACQR"
     gene            8011..8205
                     /locus_tag="VIS73_03670"
     CDS             8011..8205
                     /locus_tag="VIS73_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEY5762284.1"
                     /translation="MSNDSQCVGVCMIDPDTGACLGCGRLPEEIHGVPVATAPSAAPT
                     ASTASRLPPAVAAQLGEGTQ"
     gene            8202..9116
                     /locus_tag="VIS73_03675"
     CDS             8202..9116
                     /locus_tag="VIS73_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5762285.1"
                     /translation="MSGLPPTIRVFERGWLSSNNILLFDGADAATLIDSGYIKHAAET
                     VQIVGEALGTRRLARLLNTHAHSDHIGGNAAVQAAFGCRIAVPAGHAKPIHDWDEDAL
                     LLEPLSQRAEPFRADGVLEAGEEIVAGELAWRLIAVPGHDMDALALYNADREILIPGD
                     ALWENGFGVIFPELVGRPGGFAATRSTLDTLARLPVRWVIPGHGRPFSDVDGALTRAY
                     GRLDALESDPERFARNALKVVLSFALMDRGRIRRDDLPEFFAQSSFCAHISRNYLHRD
                     PAELGAEIARELAKVGALRRVDDCWEPF"
     gene            9194..10561
                     /locus_tag="VIS73_03680"
     CDS             9194..10561
                     /locus_tag="VIS73_03680"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY5762286.1"
                     /translation="MFKWLGGIIGGNSGTKTPAEPPLPEPEVKLREEPQPEESAGGFL
                     SRHVIINRECHPVGYLFAMRDRSRARIANLDQASARINDQLMIRAVNGLGVEKIGRYR
                     QIWLEVVDSFLSSQLIETLPAKSTVIIVRLHGSAAPDEALLVRARELRAAGFHLALSR
                     WADTAACAAWLPVIDFVAVDVSGLNPLDITEQAKKLRTAIPDTALVAMHVDSLEEFEC
                     CRGAPFAMFQGGFLTRRENWPPQPQMNPQRARLTQLMQKLQRGGELNEIASELRHSPE
                     LSYRLLRYINSAGMGLTTRIASIEQALLILGREKLYRWLTLLLFSAGPGGTIDAALLE
                     QALVRARFMELLAPESSSSLQRDELFIVGVFSLLDVLLKLPLSVALEPLALPDSSTDA
                     LLHDRGPLAPKLRLVIAAEAHDDLALSDAARELGMRVDTVNAMHMDALHWAQETLAPE
                     PADAT"
     gene            10630..12075
                     /locus_tag="VIS73_03685"
     CDS             10630..12075
                     /locus_tag="VIS73_03685"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY5762287.1"
                     /translation="MPNWLDRVTGGEATPLDTDTKAKKPAARTGEPANLREAALQAAA
                     ARPRKAKQDEPPLPPPTIMLEESDPAALSAGLLTRQVILGRKFQPEGYVFSLRAGDDL
                     AQLDPLTRDRWLLDIVDRLGVERLCRYRALWLPVSPQVLGTALLKQLPPASTVLMVDI
                     GESQPSPETMVAAALARGRGFRLCLANFRGTNPQKHWLPQADYLEIDSSRVNPLDAGV
                     IPEKLLKAGVAAGVLASHLESQEDFEFCHKANYLLFRGDFLTRREPMARNTSMSPDRI
                     TVCSLLNKLRAGAEVDEIADPFRTSPELAYRFLRYINSVGFGLTIKVASVKQGMVYLG
                     RNKLYRWLTLLLFNPEHGRSTDAALLEQALVRARMMEQLGEQQMNKVAGDELFIVGIF
                     SMLDVLLKLPMSVALDPLKLPEAVHGALVDDSGDYAPYLRLAIACEANDAQKTAEHAG
                     LLGFTVDHVNTAHFEALAFAQETLTPDPALR"
     gene            12157..12672
                     /locus_tag="VIS73_03690"
     CDS             12157..12672
                     /locus_tag="VIS73_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HEY5762288.1"
                     /translation="MTTTLIDFADPQEPKRWSSINDSVMGGLSSSRLHFDPAGHAVFS
                     GVVSFENNGGFASVRRQPDDLAACGASAYLLAVRGDGKRYKLSLRTDDGFDGISYQCG
                     FHPPAGIWTVCRLACGRFLPTRRGRVLVDEAALDPASVRQVGLLIGDRQEGEFSLTLR
                     SIGVDSAQEPA"
     gene            complement(12713..13087)
                     /locus_tag="VIS73_03695"
     CDS             complement(12713..13087)
                     /locus_tag="VIS73_03695"
                     /inference="COORDINATES: protein motif:HMM:NF016050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HEY5762289.1"
                     /translation="MRIRISSATVTLTVELNESPTAAALVKALPFSATVNTWGEEIYF
                     ATPVTAKLSADARADAAVGEVGYWPPGQALCVFFGRTPASTGSEPRAASPVNPVGRVV
                     GDAKAAAQIHDGTSVTVLAGTQ"
     gene            complement(13108..13533)
                     /locus_tag="VIS73_03700"
     CDS             complement(13108..13533)
                     /locus_tag="VIS73_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5762290.1"
                     /translation="MTLQPFHLAIPVDDLPAARRFYGEIFGLEEGRSSAQWVDFNFFG
                     HQLVIHEHPRTVAQEQAHTNPVDGHDVPVPHFGVVLGWAEWQELADRLRARGTRFVIE
                     PYIRFKGQVGEQATMFLLDPCGNALEFKAFKDIGQLFAK"
     gene            complement(13621..>13926)
                     /locus_tag="VIS73_03705"
     CDS             complement(13621..>13926)
                     /locus_tag="VIS73_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY5762291.1"
                     /translation="VAERWRYEDGSGEIGVISSVTQAFCSTCTRLRLSTDGKLYTCLF
                     ADRGHDLRSLLRSGASDTQVDRFIAAVWQQRGDRYSEIRSAETAARGAKVEMSYIGG"
BASE COUNT         2380 a         4931 c         4411 g         2204 t
ORIGIN      
        1 tgtcggctcc gatgcggccg cgatcaccac cagcgctgtc agagacggcg accactgggt
       61 gctcaacggc gtcaagcagt tcattaccac cggcaagcac gccgaagtcg cgattgtctt
      121 tgccgtgacc gacaagggcg ccggcaagaa gggcatctcc tgcttcgtca tcgaaaccgg
      181 cgcgccgcag cacatcccgg gattcgtcgt cgcccgcctc gaagacaagc tcggccagcg
      241 cgcctccgat accgcgcaga tcgtcttcga caactgccgc gtgcccgcgt cggcacttgc
      301 cggcgctgag ggcgatggct accgcattgc cctttccaac ctcgaagccg gccgcatcgg
      361 catcgccgcg cagtcggtcg gcatggcccg cgcagcactc gaagcagccg tgcgctatgc
      421 caacgaacgc gagactttcg gcaaacctat cgtccagcat caggccgtgg cctttcgcct
      481 cgccgacatg gcaacgcaac ttgaggcggc gcgacaactg gtctggcatg cggcaacact
      541 gaaggacgcc ggccaaccct gcctgaagga agcctcgatg gccaagctct ttgcctcgga
      601 aatggccgag cggctctgct ccgacgcgat ccagatccac ggcggctacg gcgtcgtcaa
      661 cgatttcccg gtcgaacgca tctaccgcga cgtgcgtgtc tgccagattt acgaaggcgc
      721 cagcgacatc cagcgcctgg tcatcgcgcg cgaggtaacg acactatgac ggctcggctg
      781 aggaccggca tcacgccgga ggatttttcg cggcgctcgg aaggcacgtt gcccggctgg
      841 tttggcgtgc gagtgacggc catcgccccc ggccggcttg acgccgaact tgacatccgc
      901 gacgagatgc tcgcccccaa cggctacctg cacgccgccg cagtaattgc gctggcggac
      961 accaccgccg gctacgcgac gatcgcgcat cttcccgagg gcgcggaaaa tttcaccacc
     1021 gtcgagctga agaccaactt ctgcgcgaca cagaccgagg gaagactttt cgccttcgcc
     1081 accgccgagc acgctgggcg gctgacgcaa atttgggacg tcgagatcat cggcgacgac
     1141 gatcggcgcc tggcgctgtt ccgctgtacg caggtgatcc tctggccgcg ggagcagaag
     1201 agatgagctc gccgatcgat ttttacttcg atttttcttc gccctacggc tacctcgcca
     1261 gcgaaaagat cgacgaactc gctgcgaagc acgggcgcac ggtgcgttgg aagccggtct
     1321 tgctcggcgt gatcttcaag accacgggcg cggcgccgct gctcaatgtg ccgctcaagg
     1381 gcgactacgc gaagatcgac atcgcgcgtt cggcgcgctc gatgaacatc ccctaccgct
     1441 tgcccgcaag ctttccgcag gcgacgcagc aggccgcgcg cgccttctat gcgcttgacg
     1501 atgccgaccc ggcgctggcg cggcgttttg cgcacgctgc ctaccgagcg atcttcgtcg
     1561 acggccgcga catctcggcg gccgacaccg tgctcgagat cgccggcgcc tgtggcgccg
     1621 ataccgaaac gctagccgcc gcgattgcca cgcccgagat caaggagcgc ctgcgccgcg
     1681 aggtcgagga ggcgatggcc gctggcgtct tcggttcgcc gtacatgatg gtcgacggcg
     1741 aacccttcat gggtgccgac cggctgccgc aactcgagcg ctggctcgcc gaagggccgt
     1801 tctgatgagg gcaattactg tcttctgcgg ctcggcgacc ggcaagcgcc cgctctatcg
     1861 tgaagccgcc gaaggcatcg gacggctgct cgcccggcgc ggcatcacgc tggtctacgg
     1921 cggtggcgac atcgggctga tgggccgcat ggccgaggcc gccctgcagg cgggcggcga
     1981 agtcatcggc gtcatccccg aggcgttgca ggcgcgcgaa ctcggccacc cgcggctgac
     2041 gcggctggaa gtcgtcgatt cgatgcacac gcgcaaggcg cgcatggccg aactcgccga
     2101 tggcttcatc gcactgcccg gcggcttcgg caccttcgag gagttctgcg aagtgctgac
     2161 ctggggccaa ctcggcttcc acaagaagcc ctgcggattg cttgatgtcg acggctacta
     2221 cgcaccgctg caggcgctgt ttggtcaggc cgagagcgag ggcttcctgc agccagaaca
     2281 tcggcgtatg gtctgcatcg accgcgacgc cgaggccctg cttgagaaaa tggccaccta
     2341 ccagccgccc gatgttgaga agtggctgag ttcggtgcgc agtctttgag ggagtgagcg
     2401 tgaccgtcat ccaatcgacg atcgataccc gcagcgacgc ctatcgtgac aacgtcgccg
     2461 ccatgcaggc ggtggtcgac gacctgcacg ccaagtcggc ccgcattgcc cgcggcggct
     2521 ccgagtcggc gcgcgagaag catctggcgc gcggcaagct gctggtccgc gatcgcatcg
     2581 acacgctgct cgacccaggc agccccttcc ttgagatcgc gcccttcgcc gcctacggca
     2641 tgtacggtgg caacagcgcg gaaagaggcg atgacttcga agtaccggcc gcctcactgg
     2701 tcgccggcat cggccgcatt gccggcgtcg agtgcatgat cgtcgccaac gacgcgacgg
     2761 tgaagggcgg cacctactac ccgctgacgg taaagaagca cctgcgcgcg caggaaatcg
     2821 cgctcgagaa ccgcctgccc tgcgtctatc tggtcgactc gggcggcgcc ttcctgccgc
     2881 tgcaggacga ggtgttcccc gaccgcgatc acttcggccg catcttcttc aaccaggcca
     2941 acctgtcggc gcttggcata ccgcagatcg ccgcagtgat gggctcgtgc acggccggcg
     3001 gcgcctacgt gccggcgatg agcgacgagt cgataatcgt gagaaatcag ggaccgatct
     3061 ttctcggcgg cccgccgctg gtgaaggccg ccaccggcga ggacgtcagc gccgaggacc
     3121 tcggcggtgc cgatgtgcac acacgcgtct ccggcgtcgc cgaccactac gccgaggacg
     3181 acccgcacgc gctggcgatc gtccgccgca tcgtcgccaa cctcaaccgg aagaagtctc
     3241 cgtcaccgac gctgaccgaa ccgcgcgagc cgctctacgc cgcccgcgag ttgtacggcg
     3301 tgatccccgt cgacagcaag aagcctttcg atgtgcgcga ggtgatcgcc cgcatcgtcg
     3361 acggctcgga gttcgacgag ttcaaggcac gctacggcac caccatcgtc tgcggcttcg
     3421 cgcgcctctg gggctatccg gtcggcatcg tcgccaacaa cggcatcctg ttttcggaat
     3481 cggcactaaa ggccgcgcac ttcatcgagt tgtgcgcgca gcgtggcatc ccgctcgtct
     3541 tcctgcagaa catcaccggc ttcatggtcg gccgcaagta cgagaacggc ggcatcgccc
     3601 gcgacggcgc gaagatggtc acggccgtgg cgactgcgaa ggtgccgaag ttcaccgtcg
     3661 tcatcggcgg cagcttcggc gccggcaact acggcatgtg cggccgcgcc tactcgccgc
     3721 gcttcctctg gatgtggccc aacgcgcgca tctcggtgat gggtggcgag caggcggcga
     3781 gcgtgctcgc caccgtgcgc cgcgacggca tagaagcggc cggcaaaacc tgggcaaagg
     3841 atgacgagga gtcgttcaag gcgccgattc gcgcccagta cgagacacag ggacaccctt
     3901 attacgccag cgcccgtctc tgggacgacg gcatcatcga tcccgccgac acgcgccgcg
     3961 tgcttggcct cggcctctcg gcagcgatga acgcgccgat cgaggaaacc cgtttcggca
     4021 tcttccgcat gtgaggaccc catgagctat gaagcaatcg tcatcgaaat agacgggccg
     4081 gtcggcatcg tcacgatgaa tcgcgccgag cggcacaacg ccttcgacga aacgctgatc
     4141 gccgaactca ccaatgccct gcaggcgctc ggcgccgatc cggcgatccg cgtcgtcgtg
     4201 ctctcctcgg tcggcaagag cttctcggcc ggcgccgatc tcaactggat gcgccgcgcc
     4261 gccgagtacg gcgacgagca gaacctcgag gacgcgcgca agctggcggc gctgatgcgc
     4321 acactcgaca cgttgcccaa gccgaccgtc gcccgcgtcc acggccccgc ctacggcggc
     4381 ggcgtcggcc tcatcgccgc ctgcgacatc gcgctggcga ccttcgatgc gcagttcgcg
     4441 ttttccgaag tcaagctcgg cctgatcccc gccgtgatca gcccctacgt gatggcccgc
     4501 atcggcgaac gctacgcgcg ccgctacttc ctcacagccg agcgcttcgc cgcggccgag
     4561 gcctatcgca tcggcctcgt ccacgaaatc gtccccgacg aggccacgct tgactcggcg
     4621 gtcggcgaag tcgtcgacat tctgctgaag aacagtccgc aggcaatggc cgctgccaaa
     4681 caactggtgc gcgcggtcag cggcgcgccg atcgacgcgc tgctgatcga ggacaccaca
     4741 cggcgcatca ccgaagcgcg caccaacccc gacgcccgcg aaggcatcgc cgcattcctc
     4801 gaaaagcggc cggcgccctg ggcaatcccc gccgccgacg aagcgcagta ggcgggccat
     4861 gttcagcaag atactgatcg ccaaccgcgg cgaaatcgcc tgccgtgtca tccgcacggc
     4921 gcaccgcatg ggcgtccgct gcgtcgcgat ccacgccgac gccgacgccg gcgcgctgca
     4981 cgtgcggcag gccgacgaag cagtgctgat tgccgccgcc gagccgcgcg ccgcctacct
     5041 cgacggcgag gcgatcatcg ccgccgccca tgccaccggt gcgcaggcga tccatcctgg
     5101 ctacggcttc ctctcggaaa atgccgactt tgccgacgcc tgcgccgccg ccggcctcgt
     5161 cttcatcggg ccaccggcct cggcgatccg cgcgatgggc gagaagagcg cggccaaggc
     5221 actgatggaa aaagccggcg taccgctgac ccccggctat cacggcgacg accaggactc
     5281 gggcctcctc gcgcagcagg ccgaacggat cggctacccg gtattgatca aggcctcgag
     5341 cggcggtggc ggcaagggca tgcgccgcgt cgacgccgcc ggcgactttg cggcggcgct
     5401 ggcgagttgc cagcgcgagg ctgcctctgc tttcggcgac gagcgcgtgc tcgtcgagcg
     5461 ctacctggcg agcgcgcggc acatcgagat tcaggtcttt gccgattcgc agggcaattg
     5521 cgtgcacctc ttcgagcgcg actgctcggt gcagcgacgc caccagaaga tcattgagga
     5581 agccccagcg cccggcctgc cggccgagac gcgggcggcg atgggcgcag cagcggtcaa
     5641 ggcggcgcag gcggtcggct acgtcggcgc cggcaccgtc gagttcatcg ccagcgccga
     5701 catcgccagg gacggcgtct tctatttcat ggagatgaac acgcgcctac aggtcgaaca
     5761 cccggtgacc gaaatgatca ccgggcagga cctagtcgag tggcagctgc gcgtcgccag
     5821 cggcgaagcg ctgccgctgg cgcaagcgca gatcgagttg cgcggccacg ccttcgaggc
     5881 acgcatctac gccgaggacc ccgagcgcga ttttctgcct tcgaccggca ccttgcactg
     5941 cctcgaactg ccggcgccga cgctgggcgc ccgcatcgac gccggcgtcg aaagaggcga
     6001 tgtgatcaca ccgcacttcg acccgatgat cgccaagctg attgtctggg atggcggcgc
     6061 cgaacgtccg cgcgacgccg cgctggcgaa gctgcgccag gcgctggccg acacgcgcat
     6121 cgttggtgtc gccaccaaca tcgccttcct ctcgcggctg gccgccaacc cggccttcgt
     6181 cgaggggcgc gtcgaaacgc ggctaatcga tcggcagcgc gagtcgctgt ttccgccgct
     6241 gacgcgcacg cccgacaccg tcgtactggt cgccactgcg gccgagctac tagctgaagc
     6301 cagccacatc gcggcgcagg cgaccgcgag cggcgacccg cattcgccct ggagcactcc
     6361 cgatggctgg cggatgaacc tcgacgcgac gcggcacctg ctgttccaca cgggcgacgc
     6421 gcgcttcgag gtcggcgtcg cctaccgcgg cgacggctgg ttgctgacgc ttaacggcga
     6481 gcaggtgccg gcccgcgccg aggcgctgcc gggcagccgc atcgccgtcg agctggacgg
     6541 ccgccggctg gtcgccagcg tcatcgccgt caatgcccgc ggcgacgcgc agcggcacgt
     6601 cttcctccac ggcgcgcgcc acctgctcca ccgcgacgat ccgctagtgc aaaccaacac
     6661 gcaggcggcg gccggcagcg ggctcaacgc gccgatgccc ggcaaggtcg tcgccctgct
     6721 ggtcgaggcg ggcagccgcg tggaacccgg cacgccgctg ctggtcattg aagccatgaa
     6781 gatggaacac acaatcaagg caccggccgc cggcacgctg cgtgcacacc gatttgcccc
     6841 cggcgacccg gttgccgacg gcgagctgct ggtgagcttc gacgccgatg cctgatccgc
     6901 ttggtttgcc gacgcgcgtc acgctggtcg aagtcgggcc gcgcgacggg ctgcagaacg
     6961 agccgacacc tgtgccggcg gcaaccaagg tggcgttgat cgggcggctc gccgcggccg
     7021 gcctgccggt ggtagaggca acctccttcg tcagtgccaa atgggtgccg cagctcgccg
     7081 acgccggcga ggtgctgcgc gcggtgctcg ccgaagcggc cgcagaggga acgcgcttcc
     7141 cggtcctgac gcccaacctc aagggcttcg acgcggcgat cgaggccggc gcccgcgagg
     7201 tagcggtctt cgcggcagcc tccgaatcgt tctcgcaaaa gaacatcaac tgctcgatcg
     7261 ccgaaagcct gaaacgcttc gagcccgtgg tcgcctcggc gcaggcactc gacatcccgg
     7321 tgcgcggcta cgtctcctgc gtcgccggtt gtccctacga gggggcggtg gcgccgggtg
     7381 cagtcgccac ggttgccgcc gcgctcgccg aaatgggctg ccacgaagtt tcgctcggcg
     7441 acaccatcgg caccggcacg ccggcatcga tcgcccgcat gctcgaagcc tgcctgcgcg
     7501 tgctgccggc cgagcgcctc gcgggccatt accacgacac ctatggcatg gcgatcgcca
     7561 acatccacgc ctcgctcacg ctgggcctcg ctaccttcga cagctcagtc gccgggttgg
     7621 gcggctgccc gtacgcccgg ggcgcgtcgg gcaacgtcgc caccgaggat gtgctctacc
     7681 tgctcgccgg gctcggcatc gaaaccggcg ttgacctcga cgcggtaatt gccgccggcg
     7741 ccttcgtctg cgaggcgctc ggtcgccgca acggctcgaa ggttgcgcag gcccggctgg
     7801 gcggacgatg atcgcctcgc cctgcgtcaa gcgctgcgcg gtcgacgaga gcggccagtc
     7861 gtgcacggcc tgcctgcgca cgctcgacga gatcatcgac tggccaacgc taaccgacga
     7921 ggacaagcgc gcggtcctgc agcgcgtcgc cgcgcggcgc agcgcgctcg aagccgcgtc
     7981 acgcgccgcc cttcccggag catgccaacg atgagcaacg actcccagtg cgtcggcgtc
     8041 tgcatgatcg accccgacac cggcgcctgc ctcggttgcg ggcgccttcc cgaggaaatc
     8101 cacggcgtgc cggtcgctac ggcaccgtcc gccgcgccga cggcaagcac cgcttcgcgg
     8161 ctgccgccgg ccgtcgccgc gcaactcggc gaaggcacgc agtgagcggc ctgccaccga
     8221 cgatccgtgt cttcgagcgc ggctggctgt cgtcgaacaa catcctgctg ttcgacggcg
     8281 ccgacgcggc gacgctgatc gattcgggct acatcaagca cgccgccgag accgtgcaaa
     8341 tcgtcggcga ggcactcggc acgcggcgcc tcgcgcggct gctcaatacc cacgcgcact
     8401 ccgaccacat cggcggcaac gccgccgtgc aggccgcctt cggctgccgt atcgcggtcc
     8461 cggccggtca cgccaagccg atccacgact gggacgagga cgcgctcttg ctcgagccct
     8521 tgtcgcagcg cgccgaaccg tttcgtgccg acggcgtgct cgaagccggc gaggaaatcg
     8581 tcgccggcga gctggcctgg cgcctgatcg ccgttcccgg ccacgacatg gatgcgctgg
     8641 ctctctacaa cgccgaccgc gagatcctga tccccggtga cgcgctctgg gaaaacggct
     8701 tcggtgtgat ctttcccgaa ctggtcggcc gacccggcgg tttcgcggcg acgcgatcga
     8761 cgctcgatac gctggcgcgg ctgccggtgc ggtgggtcat tccgggccat ggccggccgt
     8821 tttccgatgt cgacggggcc ctgacgcgcg cctatggccg gctcgacgca ctcgaatcgg
     8881 accccgagcg cttcgcccgc aacgcgctca aggtcgtcct ctcctttgcg ctgatggacc
     8941 gcggccggat ccgccgcgac gatctgcccg agttttttgc acagtcgagt ttctgtgccc
     9001 acatcagccg caactacctg caccgcgacc cggccgaact cggtgccgag attgcccgcg
     9061 aactggccaa agtcggcgcc ctacgtcgcg tcgacgactg ctgggaaccc ttctgaattg
     9121 cgggttatgc cgttcgccat agctgcatta gaatgcgcca tcacgcacag gcagtggcaa
     9181 tagaggagtc ggcatgttca aatggcttgg cggcatcatt ggcggcaaca gcggcacgaa
     9241 aactcccgcc gagccgccgc tgcccgaacc cgaggtcaag ctgcgcgaag agccccaacc
     9301 cgaggaatcc gcgggcggtt ttctctcgcg gcacgtcatc atcaatcgtg aatgccaccc
     9361 ggtcggctac ctgttcgcga tgcgcgaccg cagccgcgcc cgcatcgcga acctcgacca
     9421 agccagcgcc cgaattaacg atcagctgat gatccgcgcc gtcaacgggc tcggtgtcga
     9481 gaagatcggc cgctaccggc agatttggct cgaagtggtc gacagcttcc tgagcagcca
     9541 actgatcgag acgctgccgg ccaaatcgac tgtgattatc gtccgcctgc acggctcggc
     9601 ggcgcccgac gaggcgctgc tggtccgcgc ccgcgagtta cgcgcggcgg gctttcacct
     9661 cgcgctgtcg cgctgggccg acaccgccgc ctgcgctgcc tggcttccgg tcatcgattt
     9721 cgtcgccgtc gacgtctccg gtctcaaccc gctcgacatc accgagcagg ccaagaaact
     9781 gcgcacggcg attccagata ccgcgctggt ggcaatgcac gtcgattcgc ttgaagagtt
     9841 cgagtgctgc cgcggcgcgc ccttcgcgat gttccagggc ggcttcctga cccgccgcga
     9901 gaactggccg ccgcagccac agatgaaccc acagcgggcg cgtctgacgc agctgatgca
     9961 gaaactgcag cgcggcggag aactcaacga aatcgctagc gaacttcgcc actcgcccga
    10021 gctgtcatac cgcctgctgc gctacatcaa ctctgcgggc atggggctta ccacgcgcat
    10081 cgcctcgatt gagcaggcac tactgatcct tggccgcgaa aagctttacc gctggctcac
    10141 tctactgctg ttctcggccg gccccggcgg cacgatcgac gccgcgctgc tcgaacaggc
    10201 gctggtccgc gcacgcttca tggaactgct ggcgcccgaa tcgtcgtcca gcctccagcg
    10261 cgacgagctg ttcatcgtcg gcgtcttctc gctgctcgac gtgctgctca agctgccgct
    10321 ctcggtcgcc ctcgaaccgc tggcgcttcc cgacagctcc accgacgcac tgcttcacga
    10381 tcgcggcccg ctggcgccca agctgcggct ggtcatcgcc gctgaggcgc acgacgatct
    10441 ggcattgagc gacgcagcac gcgaactcgg aatgcgcgtc gacaccgtca atgcaatgca
    10501 catggatgcg ctgcactggg cgcaggaaac gctggcaccg gaacccgccg acgccacgtg
    10561 acccttagcc gcgcccagac cgcgatggcc atcggctaga atcggagcgg ctcaaccgga
    10621 acatcgtcca tgcccaactg gctcgaccgc gtcaccggcg gcgaagccac gccgctggac
    10681 accgacacca aagcaaagaa gcccgcagcg cggaccggcg agcccgccaa tctgcgcgag
    10741 gcggccctac aggctgccgc cgcccggccg aggaaggcaa aacaagacga accgccgctg
    10801 ccgccgccga cgatcatgct cgaggagtcg gacccggccg cgctgtcggc cggcttgctc
    10861 acccggcagg tcattctcgg tcgcaagttt cagcccgaag gctacgtttt ctcgctgcgc
    10921 gccggcgacg acctcgccca gctcgatcca ctgacacgcg accgctggct actcgacatc
    10981 gtcgaccggc tcggcgtcga acggctctgt cgctatcgcg cgctgtggct gccggtatcg
    11041 ccacaggtac ttggcaccgc gctgctcaag cagttgccgc cggcctcgac cgtgctaatg
    11101 gtcgacatcg gcgaaagcca gcccagcccc gaaacaatgg tcgctgcagc gctggcacga
    11161 gggcgcggct tccgcctctg ccttgccaac ttccgcggca ccaacccgca aaagcattgg
    11221 ctgccgcagg ccgactatct cgagatagat tcctcgcgtg tcaatccgct cgacgccggc
    11281 gtcatccccg agaagctgct caaggccggc gtcgccgccg gcgtgctcgc ctcgcacctc
    11341 gaatcgcagg aagacttcga gttctgccac aaggccaact acctgctctt ccgcggcgac
    11401 ttcctcaccc gccgcgagcc gatggcgcgc aacaccagca tgtcgcccga ccgcatcacc
    11461 gtctgctcgc tgctcaacaa gctgcgcgcc ggcgccgagg tcgatgagat cgccgatccc
    11521 ttccgcacct cgcccgaact ggcctaccgt ttcctgcgct acatcaactc ggttggcttc
    11581 ggcctgacga tcaaggtcgc ctcggtcaag cagggcatgg tctatctcgg ccgcaacaag
    11641 ctctaccgct ggctgacgct gctgctcttc aaccccgaac acggacgctc gaccgacgca
    11701 gcgctgctcg agcaggcact ggtgcgcgcg cggatgatgg aacagttggg cgaacagcag
    11761 atgaacaagg tcgccggcga cgaactgttc atcgtcggca tattctcgat gctcgacgtg
    11821 ctactcaagc tgccgatgtc ggtggcgctt gacccgctca agctgcctga agcggtacat
    11881 ggcgcgctgg tcgacgatag cggcgactat gccccctacc tgcggctggc aatcgcctgc
    11941 gaggccaacg acgcccagaa gaccgccgag cacgccggcc tgcttggctt caccgtcgac
    12001 cacgtcaata ccgcgcactt cgaagcgctt gccttcgcgc aggaaacgct cacgccggac
    12061 ccggcgctgc gttgaaaacc cggccccggc acttggcgga gcgagccatt tgtcaaacgg
    12121 ccgacaacga atcaagccat ccacgtaaca agcacaatga ccacgacact gatcgacttc
    12181 gccgatccgc aggagcctaa gcgatggtct tccattaacg actccgtaat gggcggcctc
    12241 tcgagcagcc gcctgcactt cgatccggct gggcacgcgg tcttttcggg agtggtgtcc
    12301 ttcgagaaca acggtggctt cgcctcggtt cgcaggcaac cggacgatct ggcagcctgc
    12361 ggggcttcgg cctatctgct cgccgtgcgc ggtgacggca aacgctacaa actcagcctg
    12421 cgcaccgacg acggcttcga tggaatcagc taccagtgcg gatttcatcc gcctgccggc
    12481 atctggacgg tctgccgact cgcgtgcggc cggttcctgc cgacccggcg cggacgcgta
    12541 ctcgttgacg aagccgcgct cgacccggca agcgtacgac aagtcggact gctgatcgga
    12601 gatcgccagg aaggcgagtt ctccctgacg ctgcgttcga ttggagtcga tagcgcgcag
    12661 gagcctgcct aggccagtgc tcagttatta acgggcgacc gtcgccgcct attcactgcg
    12721 tgcctgcaag caccgtcacc gatgttccat cgtggatctg cgccgccgcc ttcgcatcgc
    12781 cgacgacgcg gccgaccgga ttcaccgggc tcgcggcgcg gggttcgctg ccggtgctcg
    12841 ccggcgtccg gccaaagaac acacacagcg cctgacccgg cggccagtag ccaacctcgc
    12901 cgacagccgc gtcggcgcgg gcatcggcgc tcagcttcgc ggtgaccggc gtcgcgaaat
    12961 aaatctcctc gccccaagtg ttgacggttg cgctgaacgg caacgccttc accaacgcgg
    13021 ctgccgtcgg cgactcgttc agctcgacgg taagcgtgac cgttgccgag gagatgcgga
    13081 tacgcatggg cacgttccct gttgagacta cttcgcaaac aactgcccga tgtccttgaa
    13141 ggccttgaac tcgagcgcgt tgccgcaggg gtcgagcagg aacatcgttg cctgttcgcc
    13201 aacctgtccc ttgaaacgta tatagggctc gatgacaaag cgggtaccgc gcgcgcgcaa
    13261 acgatcagcc agttcctgcc actcggccca accgagtacc acgccgaagt gcggcaccgg
    13321 gacatcgtgg ccgtcgaccg gattggtatg ggcctgctcc tgcgccaccg ttcgcgggtg
    13381 ctcgtggatc accagctggt ggccgaagaa attgaagtcg acccactgcg cactcgaccg
    13441 cccctcttcc agcccaaata tctcgccata gaagcgccgc gcggccggca agtcgtcaac
    13501 tggaatggcg agatggaacg gttgcagtgt catcgatcac tcccgagaaa agttttcggc
    13561 cgcgaggtac cgcatggcgg ccaacgctac tcctgcccgc caacccgcgc ggcacactgc
    13621 ctagccgccg atataggaca tctccacctt ggcgccgcga gcggcggttt ccgcgctgcg
    13681 aatttcggaa tagcgatcgc cgcgctgctg ccacacggcg gcgatgaaac ggtcgacctg
    13741 ggtgtcggac gcgccactac gaagaaggct gcgcaggtca tggccgcgat cggcgaacag
    13801 gcaggtatag agcttgccat cggttgaaag gcgcaggcgc gtacaggtgc tgcaaaaggc
    13861 ttgggttacc gaggagatga cgccgatctc gccgctgcca tcttcatagc gccagcgctc
    13921 ggcgac
//
LOCUS       DATDAA010000112         2164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_287966,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000112 DATDAA010000000
VERSION     DATDAA010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287966"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..>2164)
                     /locus_tag="VIS73_03710"
     CDS             complement(89..>2164)
                     /locus_tag="VIS73_03710"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY5762292.1"
                     /translation="VPGLGQETVETAEPAPPDLVALDEDWWSYFEGAAEDVGPRIEGL
                     LTLVNEQIAGLGAQNQETAPPVLQAVRDNLQVYLSLLDGAQSEPPALDEPAAAYSLDE
                     LLDLAAEARAAREAADAAVSEVERERRVFNGASRRRDAMFKDYLDAAEGDDRWLAGLR
                     LVRARSAQAISARRLAILTQSASFAEDNADQLEARVDLVRERLATTADQEAIDGLIET
                     VAERAAAVEAAEETAREAQIAASGLDVNTAEGRSEQRLQQQRALDAEIEHSQARLALA
                     AAEARRFWTELGLETPPDFSVVEDKSLEWSELVRDVRVNLVVWKQQTEDELLAVQRVD
                     RDELDRAARRLLDQRIGTAQETLGHIGELESAAADLDLLMAAVDDSLASYAGTFEQWL
                     SNLDRAVRTAWIRISEFSGTTLFSVGETPVTFGDIVQAIVIFIVGLLLSRSIRFAIRR
                     VGSKESPGTQASLYTLSRLTHYTIVILAVLIALTSIGLDFTNLALVAGALSVGIGFGL
                     QSIVNNFVSGLIILFERSLRVGDYIELDNGLTGTVKAINVRSTLINTNDNIDIVVPNS
                     EFVSTKLTNWTLAEHILRVRIPFGVAYGSDKELVRKAAIEACDDVPYTLTHMRGREPD
                     VWLVEYGDSSLNFLLLVWVNRQGARRPTRTRAAYLWALETKLAEYGIEIPFPQRDLHL
                     RSGWPAAQD"
BASE COUNT          338 a          723 c          734 g          369 t
ORIGIN      
        1 gcacggtgat gatctcttag cgcgcagcga gcgcctgcgg ccgagagcgc gatgcccgcg
       61 agcagggcgg ctgcggctcg gaccgccgtc aatcctgcgc cgcgggccac ccggaacgca
      121 ggtgcaggtc gcgctgcggg aacgggatct cgatgccgta ctcggccagc ttggtttcga
      181 gcgcccagag ataggcggcg cgggttcggg tgggcctcct tgcgccctgg cgattgaccc
      241 agacgagcaa gaggaagttc aggctgctgt cgccgtactc gacgagccag acgtcgggtt
      301 cccgccctcg catgtgcgtc aaggtgtagg gcacgtcgtc gcaggcctct attgccgcct
      361 tgcggaccag ctccttgtca gagccgtagg cgacgccgaa aggtatgcgc acgcgcagga
      421 tgtgctcggc gagcgtccag ttggtcagct tggtcgagac gaactccgag ttcggcacga
      481 cgatgtcgat attgtcattc gtgttgatca gcgtgctgcg cacgttgatc gccttcacag
      541 tgcccgtcag cccgttatcg agctcgatgt agtcgcccac gcgcagcgag cgttcgaaca
      601 ggatgatcaa tccggagacg aagttgttga cgatggactg cagaccgaaa ccgataccga
      661 cggacagggc gccggcgacc agcgccaggt tcgtgaagtc gagtccgatc gacgtcaacg
      721 caatcaggac ggccaggatg acgatcgtgt agtgcgtcag ccggctcagc gtatagagcg
      781 aagcctgtgt accgggcgac tccttgctgc cgactcgcct gatcgcaaag cggatgctgc
      841 gcgacagcag cagcccgaca atgaatatga cgatggcttg aacgatgtcg ccgaaagtga
      901 ccggggtttc accgaccgag aacagcgtcg ttccggagaa ttcgcttatg cggatccagg
      961 ccgtcctaac cgcacggtcc aggttgctga gccactgttc gaaggtgccc gcgtagctcg
     1021 cgaggctgtc gtcgaccgcc gccatcagca gatcgaggtc ggcggcggcg ctttcgagct
     1081 cgccgatatg accgagcgtc tcctgggccg tgccgatccg ctggtcgagc agccggcggg
     1141 ccgcgcggtc gagttcgtcg cggtcgacgc gctgcacggc gagcagctcg tcctcggttt
     1201 gctgcttcca tacgacgagg tttacgcgaa cgtcgcgaac gagttcggac cactccagcg
     1261 acttgtcctc gaccaccgaa aagtccggcg gcgtttccag gccgagctcg gtccagaagc
     1321 ggcgggcctc cgccgcggcc agcgcgagcc gcgcctggga gtgttcgatt tccgcgtcca
     1381 gggcgcgctg ctgttgcagc cgctgctcgg accggccctc cgccgtgttg acgtcgaggc
     1441 cgctcgcagc gatctgcgcc tcgcgcgccg tttcctcggc cgcttcgacg gccgccgcgc
     1501 gctccgcgac ggtttcgatt agaccgtcga tggcctcctg gtcggccgtc gtcgccaggc
     1561 gttcgcggac caggtcgacc cgcgcctcga gctgatctgc attatcctcc gcgaagctgg
     1621 cgctctgcgt gagaatcgcc aggcggcggg ccgatatcgc ctgggcggac ctggcgcgga
     1681 cgagccgcag gccggccagc cagcgatcgt cgccttccgc ggcgtccagg tagtccttga
     1741 acatcgcgtc acgacgtcgg ctcgcgccgt tgaatacacg ccgctcgcgc tctacctcgg
     1801 acaccgcggc gtccgccgcc tcccgggccg cccgcgcctc cgcggcgagg tcgagtagct
     1861 cgtccaggga ataggcggcc gccggctcgt ccagggccgg cggctcactt tgcgcgccgt
     1921 cgagaagcga caggtatacc tgcaggttgt cgcgcaccgc ctgcaagacg gggggcgccg
     1981 tctcctggtt ctgggcgccg agcccggcga tctgctcgtt gaccagggta agcaagccct
     2041 cgatacgggg cccgacgtcc tcggcggcgc cctcgaagta actccaccag tcctcgtcga
     2101 gcgcgaccag gtcgggggga gccggctcgg cggtctccac ggtttcctgg ccaagaccgg
     2161 gcac
//
LOCUS       DATDAA010000113         2113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_182736,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000113 DATDAA010000000
VERSION     DATDAA010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182736"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VIS73_03715"
     CDS             complement(<1..849)
                     /locus_tag="VIS73_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY5762293.1"
                     /translation="MAADVKAPPLREYPTVPALLPDAMAGSKPYVASEKINEAIGFPG
                     KLAENWQETHDKAIGKMGELLNKYRSLQVYMDACVHCGACTDKCHYFLGTQDPKNMPV
                     ARQDLMRSVYRRYFSVAGKLFPKLVGARDLTPEVLDEWHSYFNQCSECRRCSVFCPYG
                     IDTAEVTMAAKEILDSVGYGGKYSNEIIGKVHRIGNNLGLPGPALEDTLLGLEEDVKE
                     DTGVDVRFPLDVQGADVLLITPSADFFAEPHIDSLIGYGKVFHAAGLSWTLSSKASEA
                     GNFGLFI"
     gene            complement(849..1568)
                     /locus_tag="VIS73_03720"
     CDS             complement(849..1568)
                     /locus_tag="VIS73_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEY5762294.1"
                     /translation="MSAFFALLFCAATAIFIVGVAARIVQYARTPAPLKIPTTPAPTS
                     KSGVAWRMAREVVFFESLFKGNLWIWVLGWVFHASLLLVVLRHLRYFVEPVPPFLVFL
                     QPFGTYAGLTMAAALAGLWLRRLAVERIRYISTISDHLMLVLLLAIAATGLAMRFVAH
                     TDVVAVKVFFLGLLRFDWQPLPTDPLLALHLGLVAALMIVFPFSKLLHAPGVFFSPSR
                     NQTDNPREKRHLADWAAQLER"
     gene            complement(1694..2029)
                     /locus_tag="VIS73_03725"
     CDS             complement(1694..2029)
                     /locus_tag="VIS73_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HEY5762295.1"
                     /translation="MPQLDVNGKSIEVDEEGYLVNLADWDEDVAKSIAQAESIELNEN
                     HWEVVNFLREYYEEFQIAPAVRVLTKAIGKKLGADKGNSTYLYELFPYGPAKQACKIG
                     GLPKPTGCV"
BASE COUNT          381 a          618 c          737 g          377 t
ORIGIN      
        1 gatgaacagc ccgaagttac cggcctcgga cgccttcgac gacagcgtcc aggagaggcc
       61 ggccgcgtgg aagaccttgc cgtagccaat caggctgtcg atgtgcggct cggcgaaaaa
      121 atcggccgaa ggcgtgatca gtaggacatc ggcgccttgc acgtcgagcg gaaaacgaac
      181 gtcgacgcca gtgtcttcct tgacgtcttc ctccagcccg agcagcgtgt cttcaagcgc
      241 cgggccgggc aggccgaggt tgttgccgat gcggtggact ttgccgatga tctcgttcga
      301 gtacttgccg ccgtagccga ccgaatcgag gatctccttg gccgccatcg tcacctcggc
      361 ggtgtcgatg ccgtaggggc agaacaccga gcagcgacgg cactcagagc actggttgaa
      421 gtacgaatgc cattcgtcga gcacttcggg cgtcaggtcg cgggcgccaa cgagcttggg
      481 gaagagcttg ccggcaaccg agaagtagcg gcgatagacg ctgcgcatca ggtcctggcg
      541 cgccaccggc atgttcttcg ggtcctgcgt gccaaggaag taatggcact tgtcggtgca
      601 ggcgccgcag tgcacgcagg catccatgta cacctgcagc gagcggtact tgttcagcag
      661 ctcgcccatc ttgccgatgg ccttgtcgtg ggtttcctgc cagttctcgg cgagcttgcc
      721 ggggaagccg attgcctcgt tgatcttttc tgacgcaacg tagggcttgg accctgccat
      781 cgcgtcgggc agcaaggccg gaaccgtggg gtactcgcga agcggcgggg ctttgacgtc
      841 ggcggccatc agcgttccag ttgcgcggcc cagtcggcca ggtggcgctt ctcgcgcgga
      901 ttgtcggtct ggttgcgcga cggcgagaag aaaacgcccg gcgcgtgcaa cagcttggag
      961 aacggaaaga cgatcatcag cgccgcgacc aggccgaggt gcagggcgag cagcgggtcg
     1021 gtgggcagcg gctgccagtc gaagcgcagc aggccgagga agaacacctt cacggcgacc
     1081 acgtcggtgt gggcaacgaa gcgcatcgct agcccggtag cggcgatcgc caacagcagc
     1141 accagcatca ggtgatcgga gatggtcgag atgtagcgga tgcgttcgac ggccagtcgg
     1201 cgcagccaga gcccggcaag tgcggcggcc atggtcaggc cggcgtaggt gccgaagggc
     1261 tgaaggaata cgaggaaagg cgggacgggt tcgacgaagt agcgcaggtg gcgcaggacg
     1321 acgagcagga ggctggcgtg gaagacccag cccagcaccc agatccacag gttgcccttg
     1381 aacaggcttt cgaagaagac cacttcgcgc gccattcgcc aggcaacgcc gctcttgctg
     1441 gtcggtgccg gcgtggtcgg aatcttcagt ggcgccggcg tgcgcgcgta ctgcacgatg
     1501 cgggctgcga ccccgacgat gaagatcgcg gtcgcggcgc agaacagcag ggcgaagaag
     1561 gcgctcatcg gtcgggcttc cgggtggccg gtgcggggtt aaaggggctt gctggcggtc
     1621 agcgctcgat caggtcgcgc tgcggaccgc acgcgccacc aagggcggcg tgcgcggacg
     1681 cgggcgggag cgatcagacg cagccggtcg gcttgggcag gccaccgatc ttgcaggcct
     1741 gcttggccgg tccatacgga aacagttcgt acagataggt gctgttgccc ttgtcggcgc
     1801 cgagcttctt gccgatcgcc ttggtcagga cgcggaccgc tggggcgatc tggaattctt
     1861 cgtagtactc gcgaagaaag ttaacgacct cccagtggtt ctcgttcagt tcgatgcttt
     1921 cggcctgggc gatgctcttg gcaacgtcct cgtcccagtc ggccagattg accaggtagc
     1981 cttcttcatc gacttcgatc gattttccgt taacatcgag ttgcggcatg gttctctctc
     2041 ctcttgttgc tgcggttcgg gtcgggcact gcggccgctt tcccggatgg gttgaaacga
     2101 aaacggccta gag
//
LOCUS       DATDAA010000114         3593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_427530,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000114 DATDAA010000000
VERSION     DATDAA010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427530"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VIS73_03730"
     CDS             <1..1192
                     /locus_tag="VIS73_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HEY5762296.1"
                     /translation="KSGKFVGPVDVKKIAQSVGKTSRKTCGACHFFGGGGDAVKHGDL
                     DSSMTNPPRYLDVHMDAKGLNFTCSTCHQSDGHEVAGSRVSLSAGDASNPIKRGEKNV
                     SAMGRNVASCQACHGNTPHGAAKAKDGKAVDTGRMAKLDQHTDKLACPTCHVPEFARG
                     GVATKMSWDWSTATRKDENGKPLQIKDSAGRPAYDAKKGDFTWEKDVIPEYRWLNGKS
                     RYVVMGDQIDPTKLVEINRFEGEAGAPDSRIWPLKVHRGKQPYDTENKTLAVMHTFGA
                     DDTALWKNYDFAKSIEEGMRVAGLPYSGKFDFVATEMSWPITHMVAPKEDALRCTQCH
                     TRNGRLAGVDGVYIPGQHRIATLDAAGFGIALLALLGVLGHGGIRYLAWRRNGGNHGG
                     RKAH"
     gene            1217..1828
                     /locus_tag="VIS73_03735"
     CDS             1217..1828
                     /locus_tag="VIS73_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY5762297.1"
                     /translation="MERIYVFKRFERFWHWAQALLIIFMMFTGFAIHGSHQMMAFESA
                     VELHTLAAWALITLWVFAIFWHFTTGEWKQYIPTTDKLDAMVKYYLTGIFTDAPHPFR
                     QTTLQKHNPLQRLAYLGVKLFINPLIWVTGLLYLFYNDWATWGLGALSLDTVAVLHTI
                     GAFLMLIFFIVHIYFATTGHTPTAHIKAMVTGWEEVEAEGARK"
     gene            complement(2228..3370)
                     /locus_tag="VIS73_03740"
     CDS             complement(2228..3370)
                     /locus_tag="VIS73_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY5762298.1"
                     /translation="MVFLTGFALRQALVAGRKVWYERTDESITKAQTDFSRTQQLTPS
                     ELRPWHIKRPLSVEPRDQPMFSVLIVTLPFFALVLCGYVAVRRGVLPLEAISGLNVFV
                     LFFALPCLLFRFGANTPIARLIDPTVFIVWLLCALLMVAAAVAVSRRGRINWNDAAFG
                     ALTAAFPNSGFMGVPLLVALVGERSAGPVIITIFIDLVITTSLCIALSRLDAGGPGGV
                     RAAAGRALRGVAANPMPWAIVVGALFSALELRLPGPVMNTIGLLADAASPVALFTIGA
                     VLARSQMKAGEATPIADYLPVALQKLILHPLLVLLVGWMAIQVGASLDRYTLVVLVLV
                     AALPSASNVSILAERMGADNGRIARIILASTALSFLSFAAAVALLR"
BASE COUNT          732 a         1222 c         1088 g          551 t
ORIGIN      
        1 caagagcggc aagttcgtcg gcccggtcga cgtgaagaag atcgcccaga gcgtcggcaa
       61 gaccagccgc aagacctgcg gcgcctgcca cttcttcggc ggcggcggcg acgcggtcaa
      121 gcacggcgac ctcgattcgt cgatgaccaa cccgccgcgc tacctcgacg tgcacatgga
      181 cgccaagggg ctcaacttca cctgctcgac ctgccaccag agcgacggcc acgaagtcgc
      241 cggttcgcgc gtcagcctct ccgccggcga cgccagcaac cccatcaagc gcggcgagaa
      301 gaacgtctcg gcgatgggcc gcaacgtcgc ctcctgccag gcctgccatg gcaacacccc
      361 gcacggtgcg gccaaggcca aggatggcaa ggcggtcgac accggccgca tggccaagct
      421 cgaccagcac accgacaagc tcgcttgccc gacctgccac gtacccgagt tcgcgcgcgg
      481 cggcgtcgct accaagatga gctgggactg gtcgaccgcc acccgcaagg atgaaaacgg
      541 caagccgctg cagatcaagg acagcgccgg ccgccccgcc tacgacgcca aaaagggcga
      601 cttcacctgg gagaaggacg tcatccccga gtaccgctgg ctcaacggca agtcgcgcta
      661 cgtcgtgatg ggcgaccaga tcgacccgac caagctggtc gagatcaacc gcttcgaagg
      721 cgaggccggc gcgcccgact cacgcatctg gccgctcaag gtacaccgcg gcaagcagcc
      781 ctacgacacc gaaaacaaga cgctcgccgt catgcacacc ttcggcgccg acgacaccgc
      841 actgtggaag aactacgact tcgccaagtc gatcgaggaa ggcatgcgag tggcgggcct
      901 cccctacagc ggcaagttcg acttcgtcgc caccgaaatg agctggccga tcacgcacat
      961 ggtcgcaccc aaggaagacg ccctgcgctg cacccagtgc cacacccgca acggccggtt
     1021 ggctggcgtc gacggcgtct acatccccgg ccagcaccgt atcgcgacgc tcgacgccgc
     1081 cggcttcggc attgccctgc tcgccctgct cggcgtgctc ggccacggcg gcatccggta
     1141 cctcgcctgg cgccgcaatg gtggcaacca cggcggccgc aaggctcact gacccagtcc
     1201 cggaaaggaa aagatcatgg aacgcatcta cgtcttcaag cgctttgagc gcttctggca
     1261 ctgggcgcag gcactgttga tcatcttcat gatgttcacc ggcttcgcga tccacggctc
     1321 gcaccagatg atggccttcg agtcggcagt cgagttgcac acgctcgccg cctgggcact
     1381 gatcacgctg tgggtcttcg cgatcttctg gcacttcacc accggcgagt ggaagcagta
     1441 catcccgacc actgacaagc tcgacgcgat ggtgaagtac tacctcactg gcatcttcac
     1501 cgacgcgccg caccccttcc gccaaaccac gctgcagaag cacaacccgc tgcagcggct
     1561 tgcctacctc ggggtcaagc tctttatcaa cccgctgatc tgggtcaccg gcctgctcta
     1621 cctcttctac aacgactggg cgacctgggg actcggagcg ctgtccctcg acacggtggc
     1681 cgtgctgcac accatcggcg ccttcctgat gctgatcttc ttcatcgtgc acatctactt
     1741 cgcgaccacc ggccacacgc ccaccgccca catcaaggcg atggtcaccg gctgggagga
     1801 agtcgaggcc gagggcgcgc gcaagtagat cgaaccagca accctcccgc ggcgctcggg
     1861 catcagggcg ccgcgtttcc ggccgcttgc ggccggcttt tttttggttg cgaatctact
     1921 gaagggatgc gccggtgcat tccatgtccc gaagtcccct aaccttggga cgttctcacg
     1981 cttggccagg catccggcgc gcccagcagc cgcatcgcac ctgcctcgct tgagcgctcg
     2041 aatcaggagc aggctttttg gggcggggcc atgtacgtcc gcaatgcgtt cgatgacagt
     2101 cgtccgcgcg gttcaacctt accagcagca atgcaccacg cgcatgccgc ggtcgttgct
     2161 gccgagcatg atgttggtat cgagcggcga tagatctgcg gatcggaccg ctaagcagcc
     2221 ttggggctca ccgcagcaag gctaccgcag cggcaaagct gaggaacgac agagcggtcg
     2281 aggccaggat gatgcgcgct atgcggccgt tgtccgcacc catccgctcg gccagaatcg
     2341 agacgttgct cgcgctgggc aatgcggcaa ccagcaccaa cacgaccaat gtgtaacggt
     2401 ccagcgacgc ccccacctgt attgccatcc agccgaccag caggacaagc agcggatgca
     2461 ggatcagttt ttgtaatgcg accggcaggt agtcggcgat cggagtcgcc tcgccggcct
     2521 tcatttgcga acgggcgagc acggcgccaa tggtgaacag ggccaccggc gaggcagcat
     2581 cagcgagtaa gccgatggta ttcatcaccg gaccaggcag tcgcagctcc agcgccgaga
     2641 acagggcgcc gaccacgatc gcccagggca tcggatttgc ggcgacaccg cgcaaggcgc
     2701 gcccggccgc tgcccgcacg ccccccggcc cgccggcgtc gaggcgcgac aacgcgatgc
     2761 acagcgaagt cgtgatcacc agatcgatga agattgtgat gatgaccggt ccggcagagc
     2821 gttcgccgac caacgcgacc agcagcggca cgcccatgaa gcccgagttc gggaatgccg
     2881 cggtcaatgc gccgaatgcg gcatcattcc agttgatccg gcctctccgc gagaccgcca
     2941 ccgcggcggc caccatcagc aaggcgcaca gcagccacac gatgaagacc gtcggatcga
     3001 taagccgcgc gattggtgtg ttggcgccga agcgaaacag caggcaaggc agcgcgaaga
     3061 acagcacgaa cacgttcagc ccggagatcg cctcgagcgg cagtacgccc cgccgcacgg
     3121 cgacgtagcc gcatagcact agggcgaaga aaggcagcgt gactatgagg actgagaaca
     3181 tgggctggtc gcggggctcc accgacagcg gacgcttaat atgccacggc cgcagctcgc
     3241 ttggagttag ctgttgagtg cgcgaaaaat ccgtctgggc tttcgtgatg ctttcgtctg
     3301 tcctttcgta ccagaccttc ctacccgcca ccagagcttg tcgcaacgcg aaccctgtta
     3361 aaaagaccat cgctagggag ctcgttgacg cagcccgagg ccactgcctg ggatggctgt
     3421 acggcagacc gagagttgat gtgcggagcg accggactgc tcaggcaccg gcctcgagag
     3481 cacagaaaag aacaatggga gcaacagttc ggtaagcgtg tcgcggtttt caaagcgcga
     3541 atctgctccc aagcgccgtg tgacgagggc aagctcggct agactctcga cac
//
LOCUS       DATDAA010000115         2068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_288199,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000115 DATDAA010000000
VERSION     DATDAA010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288199"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1865)
                     /locus_tag="VIS73_03745"
     CDS             complement(<1..1865)
                     /locus_tag="VIS73_03745"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5762299.1"
                     /translation="MGDLLQQVADHQADGFRLLDMQDERRRSIGALEAAFAALERRIK
                     GAGGGGILVNDSLMARPSLAELASAVEVARKDVLSELTGGGDFAAAPNGGETRLRRAL
                     DAHRAELSVSPGRHWLALQLEDFGQAVKQRRRALQLGSEMEVRQAAFAVNGDALASDF
                     RGNVEAPAWRAFTAAAQGAQQAVARSEQTLRTATYKAIGLTLLALLTTAWAITRPVRR
                     LTASTRLLASGDLATRVMRGGAREIDELSSAFNNLATELTNAELAVKVYQGQLEQRVE
                     ERTLQLRHLADHDPLTNLPNRRQLFQRLNEMLDTAAAAGSAGSHIAVLFLDLDNFKTV
                     NDTLGHEFGDRVLTEIGARLRQIAGETAVIARLGGDEFTLVFDYSGDAAEVERRAGHL
                     VGCFQRPVLIDRREVSIGASCGVAMYPEHGRDATSLLRAADAALFRAKELGRNRHCIY
                     NPSMLEVASNRFRVEQALRKAIEHRDFVLHYQPQVCLRTQRTIAVEALLRWKLNDHQI
                     LPAGEFIEIVEECGLMLDLNDWILETAAEAVSNWRRSGWPDARVAINVSAQQFVTGNF
                     LVDFERLLARHGLPPEAIELELTETMLQTGAVTVEALQELRMMKVDTALDDFGTG"
     gene            1833..>2068
                     /locus_tag="VIS73_03750"
     CDS             1833..>2068
                     /locus_tag="VIS73_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762300.1"
                     /translation="MIGNLLQQVTHRWGRLGCASIHSSTCCDRQSLRRRHHGGAVVAR
                     VGTDVSKDDSIELSEAFAQVPHGARQRFELLLHLR"
BASE COUNT          347 a          681 c          660 g          380 t
ORIGIN      
        1 aacccggtgc cgaagtcgtc gagcgcggta tccactttca tcatccgcaa ttcctgcaat
       61 gcctcgacgg tcacggcgcc agtctgcagc attgtctccg tcagctcgag ttcgatcgct
      121 tccggaggca aaccgtgccg ggcgagcagg cgttcgaaat cgaccaggaa attgccggtc
      181 acgaactgtt gcgccgacac gttgatcgcg acgcgcgcgt ccggccaacc gctgcgccgc
      241 cagttgctga ccgcttcggc agccgtctcg agaatccagt cgttcaagtc cagcatcaga
      301 ccgcattcct cgacgatctc gatgaattcg ccggccggaa ggatctgatg gtcgttgagc
      361 ttccaacgca gaagcgcctc gaccgcgata gtgcgctgcg tgcgcaggca cacctgcggc
      421 tggtaatgaa gcacgaagtc cctgtgctcg atggccttgc gcagagcttg ttccacgcgg
      481 aaacggttcg acgccacctc gagcatggat gggttgtaga tgcaatggcg attgcgaccg
      541 agttccttgg cgcgaaacag agcggcgtct gctgcccgca gcagcgaagt cgcatcgcgg
      601 ccatgttcgg ggtacatcgc gacgccgcag ctcgcgccga tagacacctc acgccggtca
      661 atgagcactg gccgctgaaa gcatccgacg aggtgcccgg cgcgtcgctc gacttcagcg
      721 gcgtcgcccg aatagtcgaa cacaagtgtg aattcgtcgc cgcccagcct cgcgatgacg
      781 gcggtttcgc ccgcgatctg gcgcaggcgt gcgccgatct ctgtcagcac ccggtcgccg
      841 aattcgtggc ccagcgtgtc gttgactgtc ttgaagttgt cgagatcaag gaacaacacg
      901 gcgatatggg atcccgcgga acctgcggcg gccgcagtgt cgagcatttc attcaggcgc
      961 tggaacaatt gccgtcggtt cggcaggttg gtgagcggat cgtggtcggc gaggtgcctg
     1021 agctgtaacg tgcgttcctc gacgcgctgc tcgagctgcc cctggtagac cttgacggca
     1081 agttcggcgt tcgtcagctc cgtggccaag ttgttgaacg cggaggagag ctcatcgatc
     1141 tcgcgcgccc cgccgcgcat cacgcgggtg gcaagatcgc cggatgccag gaggcgggta
     1201 ctcgctgtca gccggcgcac tggccgggta attgcccacg ccgtagttag caatgcaagc
     1261 agcgtaagac caatcgcctt gtaggtggcg gttctcagcg tttgctcgga gcgcgcgacc
     1321 gcctgctgtg cgccttgggc agccgcggtg aatgcgcgcc atgcgggtgc ttcgacgttc
     1381 ccgcgaaagt cggaggcgag cgcgtcgccg ttgaccgcaa atgccgcctg ccgcacctcc
     1441 atttctgaac cgagctgcag ggcccggcgt cgctgcttga ccgcctggcc gaaatcctcc
     1501 agctgcagcg ccagccagtg tcggccgggc gagaccgaca attccgcgcg gtgggcatcg
     1561 agagcccggc gcagtcgtgt ttcgccaccg ttcggcgctg cggcaaagtc gccaccgccg
     1621 gtcagctcgc tcaatacatc tttccgtgcc acctcgacgg cgcttgccag ttccgccagc
     1681 gacggtcggg ccatgaggct gtcattgacc aggatgccgc cgccgcccgc gcctttgatg
     1741 cgtcgctcca gtgcggcgaa cgccgcctcg agcgccccga ttgaccgtcg gcgctcgtcc
     1801 tgcatgtcca ggaggcggaa tccgtcggcc tggtgatcgg caacttgctg caacaggtca
     1861 cccatcgctg gggacgcctc ggctgtgcca gcatccacag ttcgacttgc tgcgaccgtc
     1921 agtcgctccg ccgccgccat cacggcggcg cggttgtcgc tcgtgtcggc acggacgtaa
     1981 gcaaggacga ctcgatcgaa ctcagcgagg ccttcgccca ggtcccgcac ggcgcgcgac
     2041 aacggttcga actgctgctg catcttcg
//
LOCUS       DATDAA010000116         3123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_497694,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000116 DATDAA010000000
VERSION     DATDAA010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497694"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1731)
                     /locus_tag="VIS73_03755"
     CDS             complement(226..1731)
                     /locus_tag="VIS73_03755"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEY5762301.1"
                     /translation="MAGENTESRDPFGSLELPSMSPAGLARKFGPGLMLMMTGIGTSH
                     LVTAPVAGGRYEYALLWCLPIAYIFKYYGFEMAIRFTHATGRSMLDAYATAWKKLPVW
                     YVLITTIIQSAVGQAGRLIAAAAVVFYFFRMYLGVDIPGFDDDGELAIYGLVLGVTSV
                     LIILRGRYAIVELATKIAAGILIVCTFAVFAVQPAPVGSFVHFFKLDTPAGSWLIIAS
                     FLGLLPTGIDVSLQASEWGKAKKVGLGRIRPELEKMGLIRKFDSFDGTSADLVVDTSS
                     FPEHAQEYCRRWFKIGLIDFRVGHVVSFVLAAIFMLLAAVWIYPSDVSGNAVMGEIAR
                     IFTDSVGPSLMLIFLAGALAATYSTAFNYFDGWPRVVGACCRNLFRKTAALPRTSREE
                     LDDAQRKTWYSEYNLYRITMLFSLVASVAIIAGLPRPVFLVLVASALAYFVAPVIYFL
                     NLYYCKTIIPKDDKLFHPSPFATWFGWFSLLVFTGMSAILIAQRLFGVSLF"
     gene            complement(1732..2715)
                     /locus_tag="VIS73_03760"
     CDS             complement(1732..2715)
                     /locus_tag="VIS73_03760"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY5762302.1"
                     /translation="MKICIYGAGAIGAWLGVELSLAGEDVTLIARGPHLEAMRQNGVK
                     LLIDGQEKIAHPKCVEDPAEAGPQDYVIITVKAHSLPGIADRLEPLLGPDTTVVTGVN
                     GIPWWYFYKLAGPYENRRLESVDPGGRVWDAVGPERAIGFVVYPAAEIAEPGVIRHIE
                     GKRYTMGEPDGQPSERCDKLCAAMQEAGFVARVRNNIRDDIWVKLWGNLSFNPISALT
                     LATLDKVAGEEGTGNVCRLMMQEAQEIGERLGASFRVSVDKRLAGGAAVGAHKTSMLQ
                     DLELGRPMEIDALVTVVQELGRLVDVPTPTIDVVLALIQQRARVAGTYPGA"
     gene            complement(2712..>3123)
                     /locus_tag="VIS73_03765"
     CDS             complement(2712..>3123)
                     /locus_tag="VIS73_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEY5762303.1"
                     /translation="AANAECFVNGWFRTGDQGVMDEDGYLTITGRLKEIINRGGEKIS
                     PREVDDCLMDHAAIQQVVTFAMPHKSLGEEVAAAVVLREGESADEASIRAFAAEHLAA
                     FKVPRKILILDEIPKGATGKLQRIGLAEKLGLGG"
BASE COUNT          567 a         1030 c          987 g          539 t
ORIGIN      
        1 tgataagggg tcagagtaaa aacttcggtt tattaagggg cccgataata aggggtcgga
       61 gtaaaaacta cgccgattgc agatgttcct tattgcgcaa agttcttact ctgacccctt
      121 ttattcagta ccccgggacg acgagagtcg gttcgagcgc aagcgcgccg tagtttgtct
      181 ctgccaggtt tttgcggagc cgcgccaata ccggtcagat cggtctcaaa acaacgacac
      241 gccgaacagg cgttgcgcga tcaggatcgc cgacatcccg gtgaacacca gcaggctgaa
      301 ccagccgaac caggtcgcga agggtgacgg gtggaagagc ttgtcgtcct tcgggatgat
      361 cgtcttgcag tagtaaaggt tgaggaaata gatcaccggc gcaacgaaat aggccagcgc
      421 ggatgcgacc agcaccagga acaccggccg cggtaacccg gcaatgatcg cgaccgacgc
      481 caccagcgag aacagcatgg tgatgcggta gaggttgtac tcggaatacc aggtcttcct
      541 ctgtgcgtcg tcgagttctt cgcgcgacgt cctcggcagc gcggcggtct tgcggaacag
      601 gttccggcag caggcgccga cgacgcgcgg ccagccgtcg aaatagttga aagcggtcga
      661 ataggtggcc gccagcgcgc cggccaggaa gatcagcatc aggctcgggc ccacgctgtc
      721 cgtgaagatc cgcgcgatct cgcccatcac ggcgttgccg ctgacgtcgc tcgggtagat
      781 ccacaccgcc gcgagcagca tgaatatcgc cgcgaggacg aatgacacga cgtggccgac
      841 acggaagtcg atcagcccga tcttgaacca gcggcggcag tactcctgcg cgtgctccgg
      901 gaagctcgag gtgtcgacga ccaggtccgc actcgtgccg tcgaaggagt cgaacttccg
      961 gatcaggccc atcttttcca gctcgggccg gatgcggccg agacctacct tcttcgcctt
     1021 gccccattcc gatgcctgca gggacacgtc gatgccggtt ggcagcaacc ccaggaaaga
     1081 cgcgatgatc agccacgaac ccgcgggcgt gtcgagcttg aagaaatgca cgaacgagcc
     1141 gaccggcgcc ggctgtacgg cgaataccgc gaatgtgcag acgatcagga tgccggcggc
     1201 gatcttggtc gccagctcca cgatcgcgta gcggccgcgc aggatgatca gcacggatgt
     1261 caccccgagc acgagcccgt agattgcgag ctccccgtcg tcgtcgaagc cggggatgtc
     1321 gacgcccagg tacatgcgga agaaatagaa gacgacggcc gcggccgcga tcagccggcc
     1381 cgcctgtccg acggccgact ggatgatcgt cgtgatcagc acgtaccaga ccggcagctt
     1441 cttccacgcg gtggcgtagg catccagcat gctgcggccg gtcgcgtgcg tgaaccggat
     1501 cgccatctcg aagccgtaat acttgaagat gtaggcgatc ggcaggcacc agagcagcgc
     1561 gtactcgtag cgaccgcccg cgaccggcgc cgtaacgaga tgactcgtgc cgatgccggt
     1621 catcatcagc atcaggcccg gaccgaattt cctcgccagc ccagccgggc tcatcgaggg
     1681 cagttcgagc gagccgaacg ggtctctgga ttccgtgttt tccccggcca tctatgcccc
     1741 cgggtaggtc ccggcaacgc gcgctcgctg ttggatcagc gcgagaacga catcgatcgt
     1801 cggcgtgggc acgtcgacga gccgcccgag ctcctgcacg accgtaacga gcgcgtcgat
     1861 ctccatcggc cggccgagtt cgagatcctg cagcatcgag gtcttgtgcg cgccgacggc
     1921 ggcgccgccc gcgagccgtt tgtcgaccga cacccggaaa ctcgcgccca gccgctcgcc
     1981 gatctcctgc gcctcctgca tcatcaggcg gcagacgttg ccggtgccct cttcgcccgc
     2041 gaccttgtcg agcgttgcga gggtcagtgc gctgatcggg ttgaagctga gattgcccca
     2101 cagcttgacc cagatgtcgt cgcggatgtt gttgcgcacc cgggcgacga atcccgcctc
     2161 ctgcatcgcc gcgcacagct tgtcgcaacg ctcggacggc tgcccgtccg gctcgcccat
     2221 cgtgtagcgc ttgccttcga tgtggcggat gacgccgggc tcggcgatct ccgccgccgg
     2281 gtaaacgacg aagccgatcg cgcgctccgg gccgaccgca tcccagacgc gaccgcccgg
     2341 gtcgacgctc tcaagccggc ggttttcgta aggcccggcg agcttgtaga agtaccacca
     2401 ggggatgccg ttgacgcccg tcacgaccgt cgtgtccggt ccgagcaggg gctcgagcct
     2461 gtcggcgatg cccggcagcg aatgcgcctt cacggttatg atcacgtaat cctgcggacc
     2521 ggcctcggcc gggtcctcga cgcatttcgg atgggcgatc ttttcctggc cgtcgatgag
     2581 cagcttgacg ccgttttgcc gcatcgcctc caggtgcggg ccgcgcgcga tcagggtgac
     2641 gtcctcgccg gcgagcgaga gctcgacgcc gagccaggcg ccgatcgctc ccgcgccgta
     2701 gatgcagatc ttcacccgcc gagccccagc ttctcggcga ggccgattct ctgcagcttg
     2761 ccggtggcgc ctttagggat ctcgtccagg atcaggattt tcctcggcac cttgaaagcc
     2821 gccaggtgct ccgcggcgaa cgcccggatc gacgcctcgt cggcgctctc gccctcgcgc
     2881 aacaccaccg ccgccgcgac ttcctcgccg agcgacttgt gcggcatggc aaacgtcacc
     2941 acctgctgga tcgcggcgtg atccatcagg cagtcgtcga cttcgcgcgg cgagatcttc
     3001 tcgccgccgc gattgatgat ctccttgagc cgtcccgtaa tcgtcaggta gccgtcctcg
     3061 tccatgacgc cctggtcgcc ggtgcggaac cagccgttga caaaacactc ggcgttggcg
     3121 gcc
//
LOCUS       DATDAA010000117         2353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_427881,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000117 DATDAA010000000
VERSION     DATDAA010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427881"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VIS73_03770"
     CDS             <1..1023
                     /locus_tag="VIS73_03770"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEY5762304.1"
                     /translation="FRIGAEQLRERVDSGAAGGQPGPRTDSDLPLRFVGVALIGILLV
                     MALVPQVFGELGHVAIRLIAALCVIVFAFFFVTVSSRIVGLVGVTSNPTSGMTIASLL
                     GTAGIFLVMGWTDITGKAAALTVGCVVAIAASIAGDTSQDLKTGFLLGATPRRQQTGE
                     LIGVLTSATFVCLSVILLAKTFGFGTAELPAPQATMMKLVIEGVLDQQLPWGFVGIGV
                     AIALVCELLKIPSLPFAVGVYLPVSTMTPLFLGGLLRGLFEKTAESREGAANYRERGI
                     LLSSGFVGGEGLLGVGIAAVAFVQGSRPAGVGTGWLGPHWLVEFAGLLAFGFLIAGFA
                     RLVKRR"
     gene            1075..1962
                     /locus_tag="VIS73_03775"
     CDS             1075..1962
                     /locus_tag="VIS73_03775"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY5762305.1"
                     /translation="MDKNEIDDRIYDLYDEYCHGHIDRRQFFERAAMLTAGGLAAMAM
                     AEALLPNYAQAEEVSFTDERIKANWVDYPSPGGTSGRMRAYLVRPTAGEGPYPAVLVI
                     HENRGLNPYIRDVARRAAVAGFLALAPDGLAPIGGYPGNDDDGREMQRSLDQGQLLRD
                     MVNSAEFLRNHELSNGKLGATGFCWGGGATNHLAVTLGDRLAAGVPFYGRAAASEDVG
                     RIKAAMMVQYAEDDERVNAMRADFEAALKANGVRHVMHTYPGTRHGFHNNSTPRFDAE
                     AAALAWRRTVDFFGEHLRS"
     gene            1998..>2353
                     /locus_tag="VIS73_03780"
     CDS             1998..>2353
                     /locus_tag="VIS73_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY5762306.1"
                     /translation="MTDEKEKSDGGKTRPLEQVFEPRYTGIATFMRAPLADSLEDLDI
                     ALVGVPTDLGVTNRAGARHGPREIRNASSLMRVQNLAMGVNPYELCRIADIGDVRFSN
                     RFDLETQVGDIEAFYRR"
BASE COUNT          366 a          779 c          815 g          393 t
ORIGIN      
        1 tttcgcatcg gcgcggagca gttgcgcgag cgggtcgaca gcggcgctgc cggaggacag
       61 cccggtccgc ggaccgacag cgacctgccg ctccggttcg tcggcgtggc cctcatcggc
      121 atcctcctgg tcatggccct cgtgccgcag gtcttcggcg agctgggcca cgtcgcgatc
      181 cgcctgatcg cggcgctctg cgtgatcgtt tttgccttct tcttcgtgac cgtctcgtcc
      241 cgcatcgttg gcctggtcgg cgtgaccagc aacccgacca gcggcatgac catcgcgtcg
      301 ctgctgggca cggccgggat cttcctcgtc atgggctgga ccgacatcac cggcaaggcc
      361 gccgcgctga ccgtcggttg cgtcgtggcc atcgccgcgt ccatcgccgg cgacacctcg
      421 caggacctga agaccggttt cctgctgggc gccacgccgc gccggcagca gaccggcgag
      481 ctgatcggcg tgctgacctc ggcgaccttc gtctgcctgt cggtgatcct gctcgccaag
      541 actttcggtt tcggcaccgc ggagctgccg gcgccgcagg cgacgatgat gaagctcgtc
      601 atcgagggcg tgttagacca gcagctgccg tggggtttcg tcggtatcgg ggtcgccatc
      661 gctcttgttt gcgagctgct gaagataccg tcgctgccgt tcgcggtcgg cgtttacctg
      721 cctgtatcga cgatgacgcc gctgttcctg ggcggcctgc tgcgcggcct gttcgaaaag
      781 acggccgaat cgcgcgaggg cgcggccaat taccgcgagc gcggcatcct gctgtcgagc
      841 ggcttcgtgg gcggcgaggg cctgctcggc gtcggcatcg cggccgtcgc cttcgtgcag
      901 ggcagccggc cggccggcgt cggcaccggc tggctgggcc cgcactggct ggtcgaattc
      961 gcgggtctac tggccttcgg cttcctgatc gccggtttcg cccggctggt aaaaaggcgt
     1021 taaccgcggc ggccggcgca ttgcaaaaga ccatttgacg ggggaatcga cgacatggac
     1081 aagaacgaga tcgacgatcg aatctacgac ctctacgacg agtactgcca cggccatatc
     1141 gatcgccggc agttcttcga gcgggccgcc atgctcacgg ccggcggcct ggcggcgatg
     1201 gcgatggccg aggcgcttct gccgaactat gcgcaggcgg aagaggtctc ctttaccgac
     1261 gagcgcatca aggcgaactg ggtcgactat ccgtccccgg gcggcacctc ggggcggatg
     1321 cgcgcctacc tggtgcggcc gacggctggc gaagggccgt atcccgcggt actggtgatt
     1381 cacgagaacc gcgggctcaa cccctacatt cgcgacgtgg cgaggcgcgc cgctgtcgcg
     1441 ggctttctcg ccctggcgcc ggacggcctg gcgccgattg gcggctaccc ggggaacgac
     1501 gacgacggcc gggaaatgca gcgctcgctc gaccaggggc agttgctgcg ggacatggtc
     1561 aacagcgcgg agttcctgag aaaccacgaa ctgtcgaacg gcaagctggg cgcgaccggt
     1621 ttctgctggg gcggcggcgc caccaatcac ctggcggtta ccctgggaga ccggctggcg
     1681 gccggggtgc cgttttacgg ccgcgcggcg gccagcgagg acgtgggcag aataaaagcg
     1741 gccatgatgg tgcagtacgc cgaggacgac gagcgcgtca acgccatgcg cgccgatttc
     1801 gaggcggcgc tgaaagccaa cggcgtgcgc catgtcatgc acacctaccc cggcacccgg
     1861 cacggcttcc acaacaattc gacgccgcgc ttcgacgcgg aagcggccgc gctggcctgg
     1921 cggcggaccg tcgacttctt cggcgagcac ctgcgtagct gacggcaggc gcggactttt
     1981 tcgaagggag agacagcttg accgacgaga aggaaaagag cgacggcggc aagacccggc
     2041 ccctggagca ggtcttcgag ccgcgctaca ccgggatcgc gaccttcatg cgggcgccgc
     2101 tcgccgattc gctcgaggat ctcgatatcg cgcttgtcgg cgtgcccacg gacctgggcg
     2161 tcaccaacag ggccggcgcg cggcacgggc cgagggaaat ccggaacgcc tcgagcctga
     2221 tgcgcgtgca gaacctggcc atgggcgtca atccctacga actctgccga atcgccgaca
     2281 tcggtgacgt ccgcttcagt aaccgcttcg atctggaaac ccaggtcggc gacatcgaag
     2341 cgttttaccg gcg
//
LOCUS       DATDAA010000118         6945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_78361,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000118 DATDAA010000000
VERSION     DATDAA010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78361"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VIS73_03785"
     CDS             complement(<1..500)
                     /locus_tag="VIS73_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY5762307.1"
                     /translation="MALPLAMAFAIASGAKPEQGLYTAIIAGFLVSALGGSRLQIAGP
                     TGAFIVVLAAITAKFGYDGLLIATLMAGVILVLLGVARMGVIIRYIPRPVIVGFTAGI
                     GVIIFVGQWSYFLGIAPATATHFHEKFGQLISHAAGFHPITTALGLLSLLLVLWSQRI
                     PGLERVP"
     gene            complement(1013..1183)
                     /locus_tag="VIS73_03790"
     CDS             complement(1013..1183)
                     /locus_tag="VIS73_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762308.1"
                     /translation="MARQLVLLQVDFADPFVGRRVSDIDRVGVPRHCLVGVNLGSVVM
                     VRPVVVVRCADV"
     gene            1428..2216
                     /locus_tag="VIS73_03795"
     CDS             1428..2216
                     /locus_tag="VIS73_03795"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEY5762309.1"
                     /translation="MKPSRWLLLAAALLVTSQAFGHGMSEAEKQSIIDGGNWHYLWLG
                     ATHMLSGYDHLAFVFGIVFFLTSFKEIVKYITAFTIGHSVTLIYATFNGIQINYFLID
                     AVIGLSVAYIAFHNLDGFRRLLDIKAPSLLAMITGLGLIHGLGLSTRLQQLPLNQDQL
                     LMNIIAFNVGVEAGQIIALAIMLMFLATWRKEPSFKPFSVAANYTLIGTGAFLFLMQM
                     HGYAHTSNPDEFGFSADNHIHEHLRMDEAAAASAPVVNRHESID"
     gene            2284..2634
                     /locus_tag="VIS73_03800"
     CDS             2284..2634
                     /locus_tag="VIS73_03800"
                     /inference="COORDINATES: protein motif:HMM:NF040442.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6488 family protein"
                     /protein_id="HEY5762310.1"
                     /translation="MLKTLIAFIVSSLVTIAPVFAGEGHTHGPGGHTHGVSMVKVTEM
                     AVAQVKSLVERGKIDKSWIDVPPAKTQKQAYDSGTEWVVTFKNDKVADAAKQTLYVFY
                     TLNGKYLATNFTGK"
     gene            3015..4085
                     /locus_tag="VIS73_03805"
     CDS             3015..4085
                     /locus_tag="VIS73_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY5762311.1"
                     /translation="MTDLFSPLRLGAIEVPNRIFMAPLTRCRAGDSHVANSLMAEYYA
                     QRAGAGLIIAEATMAMAGNSAFWREPGIHSAEQVAGWRLVTDAVHAEGGRIFLQLWHG
                     GRACHPLLNDGRQPVAPSPLAIRGEEVHTPAGKQPYVLPRELADSELPGIVEGFRVAA
                     GNARDAGFDGVEVHGANGYLLDQFLRDGTNRRSGPYGGSMENRARLLLEVLEAVCAVW
                     GGNRVGLRISPLNSYNDMLDSDPVGLAGWLAERLNEFKLAYLHLMRSDFFGKQQGDVV
                     TPVRAAYRGVLVGNMGYTADEATAAVGTGMLDAVAFGTAFLANPDLPRRFRAGAPLNE
                     PDMSTFYTPGPAGYTDYPVWAG"
     gene            4176..5309
                     /locus_tag="VIS73_03810"
     CDS             4176..5309
                     /locus_tag="VIS73_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEY5762312.1"
                     /translation="MELEGRVELVVDWDGRAIVRASVRSRRAQVAARLLRGLPPDAAA
                     ARVPLVFSLCARAQALAARLALLQAQGQTMEADERRRRDVAVAAETVHEALWRMLRDW
                     PEWLGEAAATAAMVTMRRALEPLLRGRAEVVTAAPEAATHCRGLLEEIVLGEPLAAWR
                     ARADSAGGLAQWAETGATSAARLVAAAADLPTAPAPRCLPASGAANFVERLAERIDDE
                     AFAAAPDWDGLPAETGVAQRQATLLKHGGAAARLLARIADAARAIDALAALSSDSSHG
                     SAARSVGDFASEVANDSRGRGWALVDTARGQLLHVAAVAEGVTSFYRIVAPTEWNFHP
                     QGALPGLLVGAPAASRVAAERLGVLAIQALDPCVTHHVEVVNA"
     gene            5302..5643
                     /gene="hypA"
                     /locus_tag="VIS73_03815"
     CDS             5302..5643
                     /gene="hypA"
                     /locus_tag="VIS73_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEY5762313.1"
                     /translation="MHEMALAEGVLQILEDAAGKQSFSRVKTVWLEIGRLSSVEPDAM
                     RFCFDAVTRDSLAAGARLEIVEVPGSGLCVSCGKTVEIAAVYDPCPACGEFPVQPTGG
                     TEMRVKELEVE"
     gene            5928..6935
                     /gene="hypB"
                     /locus_tag="VIS73_03820"
     CDS             5928..6935
                     /gene="hypB"
                     /locus_tag="VIS73_03820"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEY5762314.1"
                     /translation="MHPHEHPDGTVHSHPHDHAGEHHHHHHAAHHGHEHVHADGTRHS
                     HAHGHEDTHHHEHPAAHEHEHAAHLGRDTHLGTHHYGHGAAGAHAPGMTQARMVQIEQ
                     DILGKNDGLAAQNRRWLADRGILALNFVSSPGSGKTTLLVKTIAALAGKPVAVIEGDQ
                     QTANDAERIRATGAPAIQINTGKGCHLDADMVGRALKTLAPVEDSVLFIENVGNLVCP
                     AAFDLGEACKVAILSVTEGEDKPLKYPDMFRAAQLMLLNKVDLLPYLSFDVEQAIAAA
                     RRVNPQIEVIQVSATSGEGFDDWLAWLAVRQAEAGRARASEIELLRRRVAELEASQPA
                     K"
BASE COUNT         1269 a         2236 c         2250 g         1190 t
ORIGIN      
        1 ggaacgcgct ccaaccccgg aatgcgctgc gaccacaaaa ccagaaggag cgagagcaag
       61 cccagggctg tggtgatcgg gtggaagccc gccgcatggc tgatcagttg cccgaacttc
      121 tcatggaaat gtgttgctgt agccggcgcg ataccgagga agtaggacca ctgacctaca
      181 aaaatgatga cgccgatacc cgctgtgaat cccacaatta ccggccgcgg gatatagcga
      241 atgatgacgc ccattcgcgc gacgccgagc aacactagga tgaccccggc catcagggta
      301 gcgatcagaa gcccgtcgta accgaatttg gccgtgattg cggccaatac gacgatgaag
      361 gccccggttg ggccggcgat ctgtaagcgg ctgccgccca gcgctgacac caggaagccg
      421 gcgatgatgg ccgtgtacag gccctgttca ggttttgcac cactggcgat cgcaaaagcc
      481 atcgccagag ggagtgccac gaccccgacg attactccgg ccacgacatt ctggatgata
      541 tgagccctag taaataggcc cgcacgatgc gcttccacta gtgcgatcac tacatcaccc
      601 acaggtcgac gttataacga gattataacg tgctgcacat tctattcttg caacctcgat
      661 ccggcttgaa ttccgattga atgcagtccc ggaggttgca gcaagggcaa taggagccaa
      721 tggtcgggtt ggcctgcgct gtccgcaacg gccgaggtgc gacctgttga tgccggcatc
      781 aagcaagtgc tcggccgttg cggtctgtca cgctgagccg tgtatgcggc cgctgttggc
      841 cggctggcag acccttcaag ttgcactcac gcccctcaaa cggtaacttg gcatccgctt
      901 atcgatcgat gtcctcaccg tctgccaccg catgatcagg tctcgctcaa acccttggtt
      961 gttggtcttc ctgctgctgg tgatcgtcac cagccgctgg ctgccagtgc attcacacgt
     1021 cggcgcagcg cacaaccacg acggggcgca ccatcaccac gctgccgaga ttcacgccca
     1081 ccaggcaatg ccgcggcacg ccgacgcgat cgatatcgga cacgcggcgg cccacgaaag
     1141 ggtcggcgaa gtcgacctgc aacaggacca gctggcgcgc catgccccgc tggcagacga
     1201 tctgcccgat gtggtgccat cggcgcacag gaatgacgtt ccgcgtcagt cactggcaca
     1261 ggtatttccc ctcggtcccc aatttcatcc cgcgctccga ccgccgcccc atcgcggaga
     1321 gccacgcgca ccgcccttca ccgcctgatc agagtcgtta gggttttgct tcctgacggc
     1381 gctttgccgt cgccaaccca tctgatgagg tattcatgtt ttctgcgttg aaaccatcgc
     1441 ggtggctgct gctcgcggcc gcactccttg ttaccagcca ggcattcggg cacggcatgt
     1501 cggaagccga aaagcagtcg attatcgacg gcggcaactg gcattacctg tggctgggcg
     1561 ccacgcacat gctgtcgggc tacgaccatc tggcctttgt cttcggcatc gtgttcttcc
     1621 tcacgagctt caaggaaatc gtcaagtaca tcaccgcgtt cacgatcggc cacagcgtca
     1681 cgctgatcta cgcgaccttt aacggcatcc agatcaacta cttcctgatc gacgcagtga
     1741 tcggactgag cgtcgcgtac atcgcctttc acaatctcga cggcttcagg cgcctactgg
     1801 acatcaaggc gccgagcctg ctggcgatga tcaccggact agggctgatc cacggcctcg
     1861 gcctgtcgac ccggctgcag caactgcccc tgaaccaaga ccagctgctg atgaacatca
     1921 tcgccttcaa cgtcggcgtt gaggcgggtc agatcatcgc tctggcgatc atgctgatgt
     1981 tcctcgcaac ttggcgcaag gagccatcgt tcaagccgtt cagcgtggcc gccaactaca
     2041 ccttaatagg caccggcgcg tttctatttc tgatgcagat gcacggctat gcgcatacgt
     2101 cgaaccccga tgagtttggc ttcagcgcgg acaaccacat tcatgagcat ctgcggatgg
     2161 acgaggccgc cgcagctagc gccccggtgg tgaaccgaca tgaatcgatc gactgatccg
     2221 tatcgacgga atcccaactt gggcggtcct aaccattcac aaacttcact ctaggagtcc
     2281 agcatgctta agacactaat cgcattcatc gtttcttcgt tggtcactat cgctccggta
     2341 ttcgccggag agggccacac ccatggtccg ggcggccata cccatggcgt gtcgatggtc
     2401 aaggtgaccg aaatggctgt cgctcaggtg aagtcgctgg tcgagcgcgg caagatcgac
     2461 aagtcctgga tcgacgtccc gccagcgaag acgcagaagc aggcctacga tagcggtacc
     2521 gagtgggtcg tgacgttcaa gaacgacaag gttgccgatg cggccaagca gactttgtac
     2581 gttttttaca cgctgaatgg caaatatctg gcgacgaact tcaccgggaa gtgaggtcgt
     2641 agggcggggg cggtacgccg ttcccgcgcc caaaaagtca gtgcgggttg cgacggctca
     2701 tccgtgagaa tacggagtcc gataccctgg cgtgagtcac atcggattta gctgcctcat
     2761 gtcagctagt ttagttgcaa tgcatcccgc gagagaccgc agatcaagag gaggcggccc
     2821 ctctgagtga cggtttcagg gagcgctcga ccgtccgctc ctggccgaac tcggaccgca
     2881 tgtacccatc agacacgcat gaggacacga tactgcggtg cgccgttggc gatcaagcct
     2941 gatgcgccga ttgccgaaac ttttcaatcg ttgagccgac caagggtctc tcgtcgactt
     3001 ccggagcatt gcgcatgacc gatctatttt cgccgttgcg ccttggcgcc atcgaggtgc
     3061 ccaaccggat cttcatggct ccgctgaccc ggtgccgcgc cggcgacagc cacgtcgcga
     3121 attcgctgat ggccgagtac tacgcgcagc gcgctggggc cgggctgatc attgccgagg
     3181 cgacgatggc tatggccggc aattcggcgt tctggcgcga gcccggcatt cactcggccg
     3241 aacaggtcgc gggctggcga ctggtgaccg atgcggttca tgccgagggc ggccggatct
     3301 ttttgcagct ctggcatggc ggccgcgcct gtcatccgct gctaaacgac ggtcggcaac
     3361 cggtcgcgcc gagtccgctg gcgattcgcg gcgaggaggt gcatacgcca gcgggcaagc
     3421 agccgtatgt gttgccacgc gaactcgctg acagcgaact gcccggcatc gtcgaggggt
     3481 tccgcgtggc cgcgggcaac gcgcgcgacg cggggttcga tggcgtcgaa gtgcatggcg
     3541 ccaacggcta tctgctagac cagttcctgc gcgacggcac caaccgacgc agcggcccct
     3601 acggcggctc gatggaaaac cgggcgcgcc tgctgctcga agtgctagaa gccgtctgcg
     3661 cggtctgggg cggcaatcgc gtcgggctgc ggatctcgcc gctgaacagc tacaacgaca
     3721 tgcttgacag cgatccggtc ggcctcgccg gatggctggc cgaacgactc aacgagttca
     3781 aactggccta cctgcatctt atgcgcagcg acttcttcgg caagcagcag ggcgacgtgg
     3841 tgacgccggt tcgcgccgcc taccggggcg tgctggtcgg caacatgggc tatacggccg
     3901 acgaggcgac ggcggcggtc ggcaccggaa tgctcgacgc cgtcgccttt ggcacggcct
     3961 tcctggccaa tcccgacctg ccgcggcggt tccgtgccgg ggcgccgctc aacgagccgg
     4021 acatgtccac tttctacacg ccgggccccg ccggctatac cgactacccg gtttgggccg
     4081 gatagggtcg acgcgccgcc aagctaaccc tagactccga tgcctcggcc cgaggcgatt
     4141 cgtttgcgct tcgcggcgcc tttagggagg cagatttgga gctggagggg cgtgtcgaac
     4201 tggtggtcga ttgggacggt cgcgcgattg tgcgggcctc ggtccgctcg cggcgggcgc
     4261 aggtcgccgc ccggctgctg cggggcctgc cgcccgacgc ggcggcggct cgcgtgccac
     4321 tggtgttctc gctgtgcgca cgagcgcagg cgctggctgc gcgactcgcg ctgctgcagg
     4381 cgcaggggca aacgatggaa gccgacgaac gacgccgacg cgatgtcgcg gtggccgccg
     4441 agaccgttca cgaggcgctg tggcgcatgc tgcgcgactg gcccgaatgg ctaggcgaag
     4501 cggcggctac ggcggcgatg gtgacgatgc gccgggcact tgaacccctg ctgcgcggtc
     4561 gtgccgaagt agtgacggcg gcgcccgagg ccgcgacgca ctgccgtggt ttgcttgaag
     4621 aaatcgtgct cggcgaaccg ctggccgcat ggcgcgcgcg cgccgattcg gcgggcggtc
     4681 tggcgcagtg ggccgagacc ggcgcgacgt cggccgcgcg cctcgttgcc gccgcagcgg
     4741 acttgcctac ggcgccagcg ccgcgctgct tgccggctag tggcgccgcc aatttcgttg
     4801 agcgattggc ggagcgcatc gacgatgagg ccttcgcggc tgcacccgac tgggacggtt
     4861 tgccggccga gaccggcgtc gcgcagcggc aagccacgtt gctgaaacac ggcggcgcag
     4921 cggcaaggct gctcgcccgc atcgccgacg cggcgcgggc gatcgatgcg ctggccgctc
     4981 tgtccagcga tagctcgcac ggcagtgcgg cccggtcggt cggcgatttt gccagcgagg
     5041 ttgccaacga ttcgcgcggc cgcggctggg cgctggtcga cacggcgcgc ggacaattgc
     5101 tgcatgtcgc ggcggtagcg gagggcgtca cgtccttcta ccggatcgtc gccccgaccg
     5161 aatggaattt tcatccgcaa ggtgcgctgc cgggcctgct cgtcggcgcg ccggcggcgt
     5221 cgcgcgtggc ggccgagcgc cttggcgtgc tggcaatcca ggcgctcgat ccttgtgtca
     5281 cccatcacgt cgaggtggtc aatgcatgaa atggcactcg ccgaaggcgt gctgcagatt
     5341 ctcgaagatg cagccggcaa acaatcgttc tcgcgcgtga agacggtttg gctggagatt
     5401 ggtcgcctgt cctcggtcga acccgatgcg atgcgcttct gcttcgacgc ggtgacgcgc
     5461 gacagcctcg ctgccggagc acggctggag atcgtcgagg tgccgggcag cgggctgtgc
     5521 gtgagttgcg gcaagaccgt cgagatcgcc gcggtctacg acccctgtcc ggcgtgcggc
     5581 gaattcccag tgcagccgac cgggggaacc gaaatgcggg tcaaggaact cgaagtagaa
     5641 tgagcccccg ccccggcagg ccacgccgcc gcgggcttgg acgagatcaa gcgcgatagc
     5701 gagcggccgc ctgcgggtgg tcgtcagagg agatgaccat gtgcacgact tgcggctgcg
     5761 gtgccggcga aacccgaatc gaaggacagg ccgtgccgcc tgagcacgcc gatgatccgc
     5821 actggcacgt tcacgccgac ggcaccgcgc acagccacgc agcggggcac ggctacaaag
     5881 ggaaaggcca cgcgcacgag cacggtgccg accgtggcca tgcccatatc catccgcacg
     5941 agcacccgga cggcaccgtg catagccacc cccatgacca cgccggtgag catcatcacc
     6001 atcaccacgc ggcgcaccac gggcatgagc acgttcacgc cgacggcacg cgacacagcc
     6061 atgcccacgg tcacgaagat acgcatcacc acgaacatcc ggccgcgcac gaacacgagc
     6121 acgccgcaca tctggggcgt gacacgcacc tggggaccca ccattacgga catggcgcgg
     6181 ccggcgccca cgcgccaggg atgacgcagg cgcgaatggt gcagatcgag caggacatcc
     6241 tcggcaagaa cgatggcctt gccgcgcaaa accgccgctg gctagccgat cgcggcattc
     6301 tcgcgctcaa cttcgtctcc agccccggct cgggaaagac gacgctgctg gtgaagacga
     6361 tcgccgcgct ggccggcaaa ccggtggcgg tgatcgaggg cgaccagcag acggcgaacg
     6421 acgccgagcg catccgtgct accggcgcgc cggcgatcca gatcaacacc ggcaagggtt
     6481 gtcacctcga cgccgacatg gtgggccggg cgctgaaaac actggctccg gtcgaggaca
     6541 gcgtgctctt catcgagaac gtcggcaacc ttgtctgccc ggctgccttc gaccttggcg
     6601 aagcttgcaa ggtggcgatc ctctcggtca ccgagggcga ggataagccg ctcaaatatc
     6661 ccgacatgtt ccgcgccgcg cagctgatgc tgctgaacaa ggtcgacctg ctgccctacc
     6721 tgagcttcga cgtcgagcag gccatcgctg cggcgcgccg cgtcaatccg cagatcgagg
     6781 tgatccaggt ttcggcgacc agcggcgagg gtttcgacga ctggctggcg tggctggcgg
     6841 tgcggcaggc ggaggcaggc cgcgcgcgcg cctcggaaat cgagctgctg cgccggcggg
     6901 tggccgaact ggaggccagc cagccggcga agtagcgtgg ccgag
//
LOCUS       DATDAA010000119         2044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_183407,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000119 DATDAA010000000
VERSION     DATDAA010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183407"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VIS73_03825"
     CDS             complement(<1..581)
                     /locus_tag="VIS73_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762315.1"
                     /translation="MTEDSIPPDDTQRETAAAASRRAADAMALKFAESRLKKVCVARH
                     PVAEGEDETIYIDAAPPPGGDIATTATEPPPSPLTPAQRKHRLDLIAFRKSFAEFPGG
                     EPRSQVAIGDLKRGDVLRIRTIVGNVFLRIVDRIREHGSHAGEILCECHYDLGDQCNP
                     AHAASITLPICSKDHVITGADGTQRQASRKLKIR"
     gene            complement(658..1431)
                     /locus_tag="VIS73_03830"
     CDS             complement(658..1431)
                     /locus_tag="VIS73_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5762316.1"
                     /translation="MSETVFITGASSGIGAALARAYAARGAIVGLGARRGELLDALIA
                     TLPSPQRHRRYVLDVTDAAALHAAAADFCSSVGVPDIVVANAGISAGTLTEHQEDLVA
                     FRRVIEVNVFGLAATFAPFAAAMRGRGSGRLVGIASVAGIRGLPGAEAYSASKAAAIS
                     YLESLRLELRSSGVKVVTIAPGYIESPMTAVNPYPMPFILPVDVAARRFIRAIDRGTS
                     YTVIPWQMGFVAKALRLMPNWLYDRLFARAPRKPRGLPV"
     gene            complement(1493..>2044)
                     /locus_tag="VIS73_03835"
     CDS             complement(1493..>2044)
                     /locus_tag="VIS73_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEY5762317.1"
                     /translation="PAQQTATQGKIEVLEFFSFGCIHCYRLHGVAKDWAAKQAADVEF
                     VRIPVTFDRKQMVPMAKLFYALELTGDLDRLDTAVFQAVHDQGVGMADDDAVLGWIAK
                     QGVDMKKFTDAYKSFSVASKVKRGDQMTKAYNVKGTPAVYVAGRYAVRNEGMSSYEQI
                     MTTAGQLVDKVRAEQPKSRGIRG"
BASE COUNT          322 a          623 c          735 g          364 t
ORIGIN      
        1 cgtatcttca gcttgcgact ggcctgccgc tgcgtgccgt cggcgccggt tatgacgtgg
       61 tccttggagc agatcggcag tgtgatgctc gcggcgtggg cggggttgca ctggtcgccg
      121 aggtcgtagt ggcactcgca gaggatctcg ccggcgtgcg aaccgtgctc gcggatgcgg
      181 tcgacgatgc gcaggaagac gttgccgacg atcgtcctga ttcgcaacac atccccgcgt
      241 ttcaggtcac cgatggccac ctgtgagcgc ggctcgccgc ccggaaattc ggcgaaggac
      301 ttgcggaacg cgatgagatc gaggcggtgt tttcgctgcg ccggcgtcag cggcgacggg
      361 ggcggctcgg tcgcagtagt ggcaatgtcg ccgccgggtg gcggggcggc gtcgatgtag
      421 attgtttcgt cctcaccttc ggcgaccggg tggcgcgcga cacagacctt tttcagccgt
      481 gattcagcga acttgagggc catggcatca gcggcgcgcc ggctggccgc cgctgcggtt
      541 tcgcgctggg tatcgtcggg tggaatgctg tcttcggtca tgggtccagc gggtgagtat
      601 ttcgacggtt gcatcgacgg tcaagcgcgc gggccttgcc ggccgtcgga atagtcatca
      661 gaccggcagg ccgcgcggct tgcgcggtgc cctggcgaac agccggtcgt agagccagtt
      721 cggcatcaag cgcagcgcct tggcgacgaa gcccatttgc cacgggatca cggtgtaact
      781 cgtgccgcgg tcgatggcgc gaatgaaacg ccgcgcggcg acgtccacgg gcaggatgaa
      841 gggcatcgga taggggttga ccgcggtcat cggcgactcg atgtagcccg gcgcgatggt
      901 gacgaccttg acgcctgacg aacgcaactc caggcgcagt gattcgagat agctgatcgc
      961 cgcggccttc gaggcgctgt aggcttcggc gccgggcagg ccgcgaattc cggcgaccga
     1021 agcgatgccg accagccggc ccgagccacg gccacgcatg gccgctgcga agggcgcgaa
     1081 ggtggcggct aggccaaaaa cgttaacctc gatgacgcgg cggaaggcca cgaggtcttc
     1141 ttggtgttcg gtcagcgtgc ccgccgagat gccggcgttg gcaacgacga tgtcgggtac
     1201 accgacgctg ctgcagaagt cggccgctgc ggcgtgcagc gcggccgcgt cggtgacatc
     1261 gaggacgtag cgacgatgcc gctgcggact gggcagcgtc gcgatcagcg cgtcgagcaa
     1321 ctcgccgcgc cgcgcaccaa ggccgacgat ggcgccacgc gccgcgtagg cgcgggccag
     1381 tgcggcgccg atgccgctcg atgcgccggt gatgaagacc gtttcgctca ttgcctcatc
     1441 ctcgaaacga aaacgcccgc acgcggcggg cgtcgccggt ggccggggtg cctcagccgc
     1501 ggatgccgcg cgacttgggt tgttcggcgc gaaccttgtc gacgagctgg ccggccgtgg
     1561 tcatgatctg ctcatagctc gacatgcctt cgttacgtac cgcgtagcgg cctgcgacgt
     1621 agacggccgg cgtgccctta acgttgtagg ccttggtcat ctggtcgccg cgcttgacct
     1681 tgctggcgac gctgaaggac ttgtaggcat cggtgaactt cttcatgtcg acgccctgct
     1741 tggcgatcca gccgaggacc gcgtcgtcgt cggccatgcc gacgccctgg tcgtgtacgg
     1801 cctggaagac ggcggtatcg agacggtcga gatcaccggt cagttcgagc gcgtagaaga
     1861 gcttggccat cggcaccatc tgcttgcggt cgaaggtcac cggaatccgc acgaactcga
     1921 cgtcggcagc ctgtttagcg gcccagtcct tggcgacgcc gtgcaggcgg taacagtgga
     1981 tgcagccgaa ggaaaagaac tcgaggactt cgatctttcc ctgcgtggcg gtttgctgcg
     2041 ctgg
//
LOCUS       DATDAA010000120         2903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_463195,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000120 DATDAA010000000
VERSION     DATDAA010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463195"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..496)
                     /gene="lspA"
                     /locus_tag="VIS73_03840"
     CDS             complement(2..496)
                     /gene="lspA"
                     /locus_tag="VIS73_03840"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY5762318.1"
                     /translation="MPPRRENRWRLPHWLMLAALLVVADQGSKAWALQTLSAAEPIVL
                     TPFLNFVLVFNRGAAFSFLAGADGWQRWFFTVLAIVVSAWIIHTIRRYPEALRMNLAL
                     SMILGGAIGNVIDRLRLGAVVDFIDVHAAGWHWPAFNIADSAIVVGAALLIWDQLRTR
                     HDPH"
     gene            complement(496..>2903)
                     /gene="ileS"
                     /locus_tag="VIS73_03845"
     CDS             complement(496..>2903)
                     /gene="ileS"
                     /locus_tag="VIS73_03845"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY5762319.1"
                     /translation="GEQVERQKKDFIRLGVLGDWGNPYLTMNFKAEADEIRALGKLLE
                     KGYVFQGLKPVNWCIDCGSALAEAEVEYEDKTSPAIDVAFPVHDNHADRLAKAFGLTH
                     LRGPAFVVIWTTTPWTLPANEAVCAHPDLRYDLVETEKGALILVHELAEAALARYGLK
                     GKTLASCEGKALEHLLLRHPFQDRDVAVILGAHVTTEAGTGLVHTAPAHGVDDFQVGK
                     TYGLPVNNPVGDDGRFISTAEHFAGKLVWEANPLVLEALEARGHLLKAEKLRHSYPHC
                     WRHKTPIIFRATTQWFVGMDHQPSADAPSLRWVAEKAVGETEFFPAWGRARLESMIKG
                     RPDWCVSRQRNWGVPIPLFLHRETQTPHPRTPELIEAVAKRVEAQGIEAWFALDAVDL
                     LGVEAADYRKMSDTLDVWFDSGTTHFSVMRGSHAAECAYPADLYLEGSDQHRGWFQSS
                     LLTGCALDGRAPYKALLTHGFVVDGQGRKMSKSMGNVIAPQKVSDTLGAEILRLWVAA
                     TDYSGELSISDTILKQVVEGYRRIRNTMRFLLANTTDFDATRDMLPVAEWVEIDRYAL
                     AMTREMQLRVAGDYERNEFHRVIQALQVFCSEDLGGFYLDILKDRLYTTGKTSHARRS
                     AQSAIWHIAQVLTRLMAPVLSFTAEEIWRVLSGRDDDSVMLHTWHELPAAADEAALLG
                     KWGLLREVRGEVTKAFEALRIDGKIGSSLQAELALGASGDRHAALASLGDDLRFVFIC
                     SQVTLAEGADSLHAAPSPHGKCGRCWHHRDDLGRADPTNHPDLCGRCHDNLFGAGESR
                     RFA"
BASE COUNT          513 a          958 c          956 g          476 t
ORIGIN      
        1 ttcaatgcgg atcgtgccgg gtccgcaact ggtcccagat cagcaacgcg gcaccgacaa
       61 cgattgccga atcggcgatg ttgaaggcgg gccagtgcca acccgccgcg tggacgtcga
      121 tgaaatcgac gacggcgccc agccgcagcc ggtcgatgac gtttccaatg gcgccgccaa
      181 gaatcatcga caacgccagg ttcattcgca gagcctctgg atagcgacgg atggtatgaa
      241 tgatccaggc cgaaaccaca atcgccagca cggtgaagaa ccagcgctgc cagccgtcgg
      301 cgccagccag aaagctgaaa gcggcgccgc gattgaatac cagcacgaag ttgagaaagg
      361 gcgtcagcac gatcggctcg gctgccgaca gtgtctgcaa ggcccaggcc ttgctcccct
      421 gatcggcaac aaccagcagc gcggcgagca tcagccagtg cggcagccgc cagcggtttt
      481 cccgacgcgg cggcatcacg cgaagcgccg cgactcaccg gctccaaaca ggttgtcgtg
      541 gcagcgcccg cacagatcgg ggtgattggt cgggtcggct cgacccaggt cgtcgcggtg
      601 gtgccagcag cgtccgcact tgccatgcgg cgacggcgcg gcgtgcagcg aatcggcgcc
      661 ctcggccagg gttacctgag agcagatgaa gacaaagcgc agatcgtcgc caagcgaggc
      721 gagcgccgca tggcggtcgc ccgaagcgcc gagcgccagc tcggcctgca gcgacgagcc
      781 gatcttgccg tcgatgcgca acgcctcgaa tgccttggtt acctcgccgc gcacttcgcg
      841 caggagcccc cacttgccga gcagcgccgc ctcgtcggct gccgccggca gctcgtgcca
      901 ggtgtgcagc atcaccgagt cgtcgtcgcg ccccgagagc acgcgccaga tttcctcggc
      961 cgtgaagctc agcaccggcg ccatcagccg ggtcaagacc tgcgcgatgt gccaaatggc
     1021 gctttgtgcc gagcggcgtg catgcgaagt cttgccggtg gtgtacagcc ggtccttgag
     1081 gatgtcgaga tagaaaccgc cgagatcctc ggagcagaac acctgcagcg cctgaatgac
     1141 gcgatggaac tcattgcgct cgtagtcgcc ggcaacgcgc aactgcatct cgcgggtcat
     1201 cgccagcgcg tagcgatcga tctcgaccca ctcggctacc ggcagcatgt cgcgcgtcgc
     1261 gtcaaagtcg gtggtgttgg cgagcaggaa gcgcatcgtg ttgcgaatgc ggcgatagcc
     1321 ttcgacgacc tgtttgagaa tcgtgtcgga aatcgacagc tcgcccgaat agtcggtcgc
     1381 ggcaacccac aggcggagaa tctcggcgcc cagcgtgtcg ctgaccttct gcggcgcgat
     1441 gacgttgccc atcgacttgc tcatcttgcg gccctggccg tcgacgacga agccgtgggt
     1501 cagcagcgcc ttgtacggcg cccgaccgtc gagcgcgcaa ccggtcagta gcgaactctg
     1561 gaaccagccg cggtgctggt ccgagccttc gagatagagg tcggccggat acgcgcattc
     1621 ggcggcgtgc gagccgcgca tcacagagaa gtgcgtcgtt cccgaatcga accagacgtc
     1681 gagcgtatcg ctcatcttgc ggtagtcggc agcctcgacg ccaagcaggt cgaccgcatc
     1741 gagcgcgaac caggcctcaa tgccctgcgc ttcgacgcgc ttggcgactg cttcgatcag
     1801 ctcgggcgtg cgcggatgcg gtgtttgggt ctcgcggtgc aggaagagcg gaatcggcac
     1861 gccccagttg cgctggcgcg acacgcacca gtcgggccgc cccttgatca tcgattcgag
     1921 gcgcgcccgg ccccaggccg gaaagaactc ggtctcgccg acggcctttt cggcaaccca
     1981 gcgcaggctc ggcgcatcgg ccgacggctg gtgatccatg ccgacgaacc actgcgtcgt
     2041 cgcacggaag atgatcggcg tcttgtgccg ccagcaatgc gggtagctgt gcctgagctt
     2101 ctcggcctta agcaggtggc cgcgcgcttc gagtgcttcg aggaccagcg gattggcctc
     2161 ccacaccaac ttgccggcga agtgctccgc ggtgctgatg aagcggccgt cgtcgccgac
     2221 cgggttgttc accggcaggc cataggtctt gccgacctgg aaatcgtcga caccgtgcgc
     2281 cggcgccgta tggacgaggc cggtgccggc ctctgtggtc acgtgggcgc cgaggatgac
     2341 cgccacgtcg cgatcctgaa acgggtggcg cagcagcaga tgctcgagcg ccttgccctc
     2401 gcaggaagcc agcgtcttgc ctttcagccc atagcgcgcc agtgccgcct cggcgagctc
     2461 gtggaccagg atcagggcgc ccttttcagt ctcgactagg tcgtagcgca ggtcaggatg
     2521 cgcgcagacc gcttcgttgg ccggcagcgt ccacggcgtc gtcgtccaga tcaccacaaa
     2581 cgccgggccg cgcaggtgtg tgaggccgaa ggccttggcc agccggtcgg cgtggttgtc
     2641 gtgaaccggg aaggcgacgt cgatcgccgg cgaggtcttg tcctcgtact cgacttcggc
     2701 ctcggccaac gccgatccgc agtcgatgca ccagttgacc ggcttcagcc cctggaagac
     2761 gtagcccttc tccagcagct tgccgagcgc gcggatctcg tcggcctcgg ccttgaagtt
     2821 catcgtcagg taggggttgc cccagtcgcc gagcacgccg agacggatga aatccttctt
     2881 ctggcgctcg acctgctcgc cgg
//
LOCUS       DATDAA010000121        11740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_114117,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000121 DATDAA010000000
VERSION     DATDAA010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11740
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114117"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VIS73_03850"
     CDS             <1..539
                     /locus_tag="VIS73_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEY5762320.1"
                     /translation="LQLAVRNGALKGVNLGRSFRDLKAKFSQGATSEVAAQSDAQTDF
                     SELTASFKVRDGVLRGDDLAAKSPFLRLGGSGEVSLPDQTLDYLLKATVVSTSTGQGG
                     DELEHLKGLTVPVRLRGPLAAPSWKIEFAGLSGEAAKAKLAEKKEEVKAKLEENKVEL
                     REQVKEKARDKLKGLLGR"
     gene            complement(517..2178)
                     /locus_tag="VIS73_03855"
     CDS             complement(517..2178)
                     /locus_tag="VIS73_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY5762321.1"
                     /translation="MKLVHGLHFGNLRGDIYGGLTAAVVALPLALAFGVASGAGPIAG
                     LYGAIFVGFFAALLGGTPAQVSGPTGPMTVIAATVIAQYAHSPAMAFTVIVMAGAFMV
                     LFAFLRLGQYIRLVPYPVISGFMSGIGCIIVLIQLGPLVGHVGEGGNPMAALANLPGQ
                     LATISWPALGLGVATLAIVYLMPPRLNRIMPSPLVALLACTLLGTAVLTGSPVIGEIP
                     TGLPTPHWPTLELAALPAMLKSALTLAALGAIDSLLTSLVADNITSTHHDSNRELFGQ
                     GIGNMAAGIMGGIPGAGATMRTVINVRAGGRTPLSGMIHALVLLAIVLGLGGLAEYIP
                     HAVLAGILLKVGTDIIDWNYLRHLRHASKGEVFLLFTVLALTVFVDLITAVAVGVVMA
                     SLQFVQRMSDEQQQNIVGIRGGADELTADLAPEEQRLLDACGDRVVVFRLSGPMSFGA
                     VHWLSRSLGVADSYRAMVLDLTEVSFLDSSAAVAIDEIVNRVRQTGGEVILVGLRPRV
                     GRVLNRLGVLRKIGPNHRFRLMRSALERARTLLRDDGGLSAPAGP"
     gene            2380..2826
                     /locus_tag="VIS73_03860"
     CDS             2380..2826
                     /locus_tag="VIS73_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5762322.1"
                     /translation="MQVREIIDIKGNALFTATPQQPLAEAVASMAENDVGSLVVMGGG
                     KMVGMLTFREIIQALNQRKGNIAGTTVADVMVANPTTAGLGMEANDLRRLLIDEHMRY
                     LPVMDGETLMGVISFLDVARAVLEEQGFENRMLKNYIRNWPSETDD"
     gene            2935..4593
                     /locus_tag="VIS73_03865"
     CDS             2935..4593
                     /locus_tag="VIS73_03865"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEY5762323.1"
                     /translation="MEKVWLKSYPSGVPAEIDPDAYGSICDVFDEALGRFGERDAYVC
                     MGKAISYQRLDELSAAFAAYLQGELKLPPGTRVALMMPNLLQYPVALFGTLRAGCVVV
                     NTNPLYTARELEHQLRDSGAEVIVILENFAHILQKILPKTAVKHVVVTSIGEMLGTLK
                     GAIVNLVIRRVKKMVPPWSLPGSTTWTAALAAGERHEFKPVPRTGSDIAFLQYTGGTT
                     GVAKGAILTHRNIIANLQQAHAWIAPWVTDGKELIVTALPLYHIFALTANCLTFMKLG
                     ATNLLITNPRDIPAFVKELGKYQFTVITGVNTLFNALLNNPEFAKLDFSNLKVTLGGG
                     MAVQKAVADRWKSTTKCTLAEAYGLTETAPAATINPLDIPEYNGSIGLPISSTLIAIR
                     SEEGADVPIGENGEIAISGPQVTQGYWQREADTREAFWPDGFFRSGDIGVMDERGFIR
                     IVDRKKDMILVSGFNVYPNEIEAVIAMHPGVMEVAAVGVPDERSGEAVKVFVVRKDPA
                     LTAEALIAHCRDSLTGYKVPHQVEFRDELPKTNVGKILRRALRE"
     gene            4876..5982
                     /gene="aroC"
                     /locus_tag="VIS73_03870"
     CDS             4876..5982
                     /gene="aroC"
                     /locus_tag="VIS73_03870"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY5762324.1"
                     /translation="MSGNSIGTLFCVTSFGESHGPAIGCVVDGCPPGMELCEGDIQAD
                     LDRRKPGTSRHVTQRREGDRVEILSGVFEGRTTGTPIALLIRNEDQRSKDYGNIAETF
                     RPGHADYTYTQKYGFRDYRGGGRSSARETAVRVAAAAIARKWLRENRGITVRGWMSAL
                     GPIEIPFADEEAIERNPFFVPDERIVPELERYMDELRKAGDSVGARLDVIARGVPPGL
                     GEPVYDRLDADIAHAMMGINAVKGVEIGAGFASVAQRGSEHGDEMSPAGFLSNHAGGI
                     LGGISSGQDIRVSIAIKPTSSIRLERKTVDRHGNPAVVATHGRHDPCVGIRATPIVEA
                     MLALVLMDHVLRHRAQCGDVRCDTPVIPGAVSGT"
     gene            6032..7207
                     /locus_tag="VIS73_03875"
     CDS             6032..7207
                     /locus_tag="VIS73_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5762325.1"
                     /translation="MSAPPLPYWRLSAYYLAYFSFLGAFAPYFGLYLQERGLSAWYIA
                     VLLSQMQLMRIVAPIAWGWIADHSGRPVAIVRASAVLSLAGMVAFLLVDSVAGWFVTI
                     AVMAFFWSAALPLVEVVTMARLGPSGAGYTRIRLWGSIGFVVAVLMIGGLIEAISLQV
                     LPVALIAMLIAVVGSSLALPGDGVRIRPEAAVRISAVLRQPRIRRLLGACFFMSLAHG
                     ALYVFYSIHLADAGYGATAIGFLWSLGVLAEIAVFRWGHRWQHLASPRVLLAVCLLAA
                     VIRFATIGWGVDFLILLIVGQLLHGLTFGAFHAVSVAAVHRWFPAGSQSRGQALYSSI
                     SFGAGGFAGGLLSGAMWDAWGGAMSYTVSAAFALAGIVVVVGPALARRLWRRRAVQA"
     gene            7261..8670
                     /gene="leuC"
                     /locus_tag="VIS73_03880"
     CDS             7261..8670
                     /gene="leuC"
                     /locus_tag="VIS73_03880"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY5762326.1"
                     /translation="MPQTLYDKLWQSHVVRQEGDGTALLYIDRHLIHEVTSPQAFEGL
                     KIAGRKPWRVSSIVATADHNTPTDHWERGIEDPISRLQVEALDTNIRAVGALAYFPFR
                     DARQGIVHVVGPENGATLPGMTVVCGDSHTSTHGAFAALAFGIGTSEVEHVLATQCLL
                     SKQSKSMRIRVDGKLGRGVGAKDIALYVIGVIGTAGGTGHAIEFAGSAIRGLSMEGRM
                     TLCNMAIEAGARAGMVAADETTIAYLKGRQFSPKGADWDRAVSYWRTLHSDEGARFDR
                     DISVDAAAIQPQVTWGTSPEMVAAIDGRVPDPVRETDPVRREGYERALNYMGLTPNMA
                     IKDIKIDKVFIGSCTNSRIEDLRVAAAVAKGRQVAANVKLALVVPGSGLVKQQAEAEG
                     LDKIFEAAGFEWRQPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGPGGRTHLVSPA
                     MAAAAALAGRFVDVRDLGV"
     gene            8673..8930
                     /locus_tag="VIS73_03885"
     CDS             8673..8930
                     /locus_tag="VIS73_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762327.1"
                     /translation="MRMLMSLAVLVATMTLAGCNTWRGLGQDVQQVGGAVGETAEKVG
                     PKIGETAEKVGQAIGRGVERVGKSIKGAADSPRDEVDRPRQ"
     gene            8940..9578
                     /gene="leuD"
                     /locus_tag="VIS73_03890"
     CDS             8940..9578
                     /gene="leuD"
                     /locus_tag="VIS73_03890"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY5762328.1"
                     /translation="MEKFVRLEGLVAPLDRNNVDTDAIIPKQFLKSIHRTGFGPNCFD
                     ELRYLDHGEPGMDCSKRPLNPAFVLNQPRYRGAKVLLTRRNFGCGSSREHAPWALLDC
                     GFRAILAESFADIFYNNCFKNGILPIVLSADQIGALFAQSTDGEGLKVVVDLPSQRVL
                     LPGGDAYGFDVDPFRKECLLNGWDDIGLTLRHADEISAFEARRQHEQPWLFA"
     gene            9606..10679
                     /gene="leuB"
                     /locus_tag="VIS73_03895"
     CDS             9606..10679
                     /gene="leuB"
                     /locus_tag="VIS73_03895"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEY5762329.1"
                     /translation="MKILVLPGDGIGPEITAEAVRVLEVLRRDGLAIELENGLLGGAA
                     VDAHDDPYPEATRRLARAADAVLLGAVGGPKWDQLPRPKRPERGLLGIRKDLNLFANL
                     RPAILYPELANSSTLKPEVVAGLDILIVRELTGDIYFGQPRGVEERDTEWGRQKVGFN
                     TMVYAESEIRRIARVAFEAAMKRGRKLCSVDKMNVLECTQFWRDIVIDVAKDYPQVEL
                     SHMLVDNAAMQLVRAPKQFDVIVTGNMFGDILSDEASMLTGSIGMLPSASLDEKNKGL
                     YEPCHGSAPDIAGQGVANPLATILSAAMMLRYTFAREDAAQRIEAAVKQVLAQGFRTG
                     DIYEPGTKRVGTREMGDAVIAAL"
     gene            10707..>11740
                     /gene="asd"
                     /locus_tag="VIS73_03900"
     CDS             10707..>11740
                     /gene="asd"
                     /locus_tag="VIS73_03900"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY5762330.1"
                     /translation="MMRVGLVGWRGMVGSVLMQRMREENDFALFEPVFFTTSNVGGAS
                     PNFGQEAPPLQDAKSIDALKAMDVIVTCQGGDYTTEIYPKLRAAGWNGHWIDAASSLR
                     MKDDAVIILDPVNLKVIKDALAKGARNWIGGNCTVSLMLMAVGALYQRDLVEWMSAMT
                     YQAASGAGAQNMRELLAQMGELNRAVKSLLDDPASAILDIDREVAGTLRAEGFPAANF
                     GVPLAGSLIPWIDKELESGQSKEEWKGQAETNKILGREASPVPIDGICVRIGAMRCHS
                     QALTIKLKKDVPLAEIEDMIRESSAWVKVVPNHRDVTMRELTPTAVTGTLTVPVGRMR
                     KLNMGPEYLGA"
BASE COUNT         2130 a         3573 c         3915 g         2122 t
ORIGIN      
        1 atttgcagct ggctgttcgc aatggcgccc tcaagggcgt gaatctcggc cgctcgttcc
       61 gcgatctgaa ggcaaagttc tcgcaggggg ccacgagcga ggtcgccgcc cagtccgatg
      121 cgcaaaccga tttctcggag ctgacggcgt cgttcaaggt tcgcgatggc gtgctgcgcg
      181 gcgacgatct ggcggccaag tcgcctttcc tgcggctagg cggaagcggt gaggtcagtc
      241 tgcccgacca gacgctcgat tacttgttga aagcgacggt cgtcagcaca tccaccggtc
      301 agggcggcga cgaactcgaa catctcaagg gcttgacggt tccggttcgc ctgcgcggac
      361 cgttggcggc gccttcctgg aagatcgaat ttgccggtct gtcgggcgaa gctgccaagg
      421 ccaagctggc agagaagaag gaagaggtaa aggcgaagct cgaagagaac aaggtagagc
      481 tgcgcgagca ggtcaaggaa aaggcgcggg acaaactcaa gggcctgctg gggcgctgag
      541 gcccccatcg tcgcggagca gcgtgcgcgc ccgttcgagc gccgagcgca tgaggcggaa
      601 gcggtggttg gggccgatct tgcgcagcac gcccagacga ttgagaacgc ggccgacgcg
      661 cggccgcagg ccgaccagga tcacctcgcc gccggtttgc cgcacacggt tgacgatctc
      721 gtcgatcgct accgcggcac tgctgtcaag gaaggagacc tcggtcaggt cgagcaccat
      781 cgcgcgatag ctgtcggcta cgccgaggct gcgcgagagc cagtgcacgg caccgaaact
      841 catcggcccg gagaggcgga agacgacgac acgatcaccg caggcgtcga gcaggcgctg
      901 ttcctcgggc gcgagatcgg cggttaactc gtcggctccg ccgcggatgc cgacgatgtt
      961 ctgctgctgc tcgtcggaca tgcgctgcac gaactgcagg ctggccataa cgacaccgac
     1021 ggcgaccgcc gtgatcaggt cgacgaacac cgtcagcgcc agcaccgtga agagcaggaa
     1081 aacttcgccc tttgacgcgt gccgcaggtg ccgcaggtag ttccagtcga tgatgtcggt
     1141 accgaccttg agcaggatgc cggcgagtac cgcgtgcgga atgtactcgg cgaggccgcc
     1201 gaggccgagg acgatggcga gcaggacgag agcgtggatc attcccgaga gcggtgtgcg
     1261 gccgccggcg cggacgttga tgacggtgcg catcgtcgcc ccggcgccgg ggatgccgcc
     1321 catgatcccg gcggccatgt tgccgatacc ttgcccaaac agctcacgat tcgaatcgtg
     1381 gtgcgtgctg gtgatgttgt cggcgaccag cgaggtgagc agcgagtcga ttgcgcccag
     1441 tgccgccagc gtcagtgcgc ttttcaacat ggcaggcagc gccgccagtt cgagcgttgg
     1501 ccagtgcggc gtcggcagcc cggtgggaat ctcgccgatc accggcgacc cggtcagcac
     1561 ggcggtgccg agcagcgtgc aggccagcag cgccacgagc ggcgagggca tgatgcgatt
     1621 gagtcgcggt ggcatcaggt agacgatcgc cagcgtcgcc acgccgagtc ccagcgccgg
     1681 ccaggaaatt gtcgccagct gtcccggcag gttggccagc gccgccatcg ggttgccgcc
     1741 ttcgccgacg tgaccgacca gcggtccgag ctggattagc acgatgatgc agccgatgcc
     1801 gctcatgaaa cccgagatga ccgggtaggg caccagccga atgtactggc cgagacgaag
     1861 gaaggcgaac aggaccatga aggcaccggc catgacgatt acggtgaagg ccattgccgg
     1921 cgaatgcgcg tactgcgcta tgaccgtcgc ggcgatcacc gtcatcggcc cggtcggacc
     1981 cgatacctgc gccggtgtgc caccgagcag cgccgcgaag aatccgacga aaatcgcgcc
     2041 gtagaggccg gcgatcgggc cggcacccga ggcaacgccg aaggccagcg ccagaggcag
     2101 ggcgacgacc gcggcggtca gaccgccata gatgtcgccg cgcaggtttc cgaaatgcaa
     2161 tccgtgaacg agcttcatcg ctggtcgaaa ctccttgtcg ggaaagcggc tattctgaac
     2221 ctgcggccgg ggaattccac tctccgcatg ggtagtgccg caagcagcgc gatgggtgct
     2281 ttgccagccg cgcgccttga actaagattg ggcgcccgat acagtgcatc cgggtcggtc
     2341 atggccccgg gaaagacatc gaatacggaa ggtaggctca tgcaggtacg tgaaatcatc
     2401 gacatcaagg gcaacgccct gtttaccgca acgccgcaac agccgctcgc agaggcggtc
     2461 gcctcgatgg ccgaaaacga tgtcggctcg ctggtggtga tgggcggcgg caagatggtc
     2521 ggcatgctga cctttcgcga aatcatccag gcgttgaatc agcgcaaggg caacatcgcc
     2581 ggcactaccg tagcggatgt catggtcgcc aacccgacca ccgccggtct gggcatggag
     2641 gccaacgact tgcgccgcct gctgatcgat gagcacatgc gctatctgcc ggtgatggac
     2701 ggcgagacac tgatgggcgt gatctcattt ctcgacgtag cgcgcgccgt gcttgaggag
     2761 cagggattcg agaatcgcat gctgaagaac tacatccgca actggccgtc cgagaccgac
     2821 gactgatctg catgacggtg gtcctgcggc caccatgcat tcggggtgcc agtccgccgc
     2881 gccccggccc gatcgacggg tcgatacgaa taaaggcggg tagaggagag caccatggag
     2941 aaggtctggc tgaaaagtta cccgtcgggc gttccggcgg aaatcgatcc agatgcctat
     3001 ggatcgatct gcgatgtctt cgacgaagcg ctcggtcgct tcggcgagcg cgatgcctat
     3061 gtctgcatgg gcaaggcgat ttcctaccag cgactcgatg aactgagtgc agcctttgcc
     3121 gcctacctgc agggcgaact caagctgccg cccggcaccc gcgtggcgct gatgatgccc
     3181 aacctgctgc agtatccggt ggccttgttc ggcacgctgc gggccggctg tgtcgtggtg
     3241 aacacgaatc ccttgtacac ggcgcgcgaa ctcgaacatc aactgcgcga ttcgggagcc
     3301 gaggtaatcg tcatcctcga gaacttcgcg cacatcctgc agaagattct gccgaagacc
     3361 gcggtcaagc acgttgtcgt caccagcatc ggagagatgc tcggaacgct caagggcgcg
     3421 atcgtcaatc tggtgattcg ccgcgtgaag aagatggtgc caccgtggtc gctgccggga
     3481 tcgacgacct ggaccgccgc gctggcagcc ggcgaacggc acgaattcaa accggtgcca
     3541 cgtaccggca gcgatattgc cttcctccag tacacgggcg ggacgacggg cgtggcgaag
     3601 ggcgcgatcc tcacccatcg caacatcatc gcgaatttgc agcaggccca tgcgtggatc
     3661 gccccgtggg taaccgacgg caaggaactg attgttacgg cgctaccgct ttatcatata
     3721 ttcgcgctga ccgcgaactg cctgaccttc atgaagctgg gcgcgaccaa cctgttgatc
     3781 accaatccgc gcgacatacc tgcattcgtt aaggagctcg gcaagtacca gtttacggtg
     3841 atcaccggcg tgaacacctt gttcaatgcg ctgctcaaca accctgaatt cgccaagctg
     3901 gatttttcca atctcaaggt cacgcttggt ggcggcatgg cggtgcagaa ggcggtcgcc
     3961 gatcgctgga agtcgaccac caagtgcacg ctggccgagg cctatggcct gaccgaaacc
     4021 gcgccggcgg cgaccatcaa cccgctcgat atccccgaat acaacggctc aattgggctg
     4081 ccgatttcgt cgacgctgat cgcgattcgc agcgaggaag gcgccgatgt gccgatcggc
     4141 gagaacggtg aaatcgccat cagcgggccg caggtgacgc agggctactg gcagcgcgaa
     4201 gcggatacgc gcgaggcctt ctggcccgac ggctttttcc gcagcggcga tatcggcgtg
     4261 atggacgaac gcggattcat tcgcatcgtc gatcgcaaga aggacatgat tctggtctca
     4321 ggtttcaacg tctatcccaa cgaaatcgaa gcagtcatcg cgatgcatcc gggggtgatg
     4381 gaagtcgccg ccgtcggggt tccggacgaa cgatcaggcg aagccgtgaa ggtcttcgtg
     4441 gtgcgcaagg acccggcact gaccgccgag gccctgatcg cgcattgccg cgacagcctg
     4501 accggctaca aggtcccgca tcaggtcgag tttcgcgacg agttgccgaa gaccaacgtc
     4561 ggcaagatcc tgcgtcgcgc gctgcgcgag tagttgagta gtaacgacgt cggcggtggg
     4621 tgcggcggaa tttcgctagc gccggccgcc cttcgcgcca cccttgtccc aaacgtagcg
     4681 cgcctggcgc tgccaccgac cgacgccagc cgcgggcggt ttcgcagttg cggcggtgtc
     4741 ggtgacagcc cgctttcggt ttcgcgctga agacgctgga ggcgttgatt taccgggcgt
     4801 tttggccatc tgacggaagg ggcgagcagg gtcgctgtgc tagactcact cccattttag
     4861 acagcctgca aatccatgtc cggaaacagt atcggcaccc tgttctgcgt gacctctttc
     4921 ggcgaatccc atggtccggc gatcggttgc gtggtcgacg gttgcccgcc ggggatggag
     4981 ctgtgcgaag gtgatattca ggctgatctg gatcggcgca agcccggcac gtcgcgccat
     5041 gttacgcagc gccgcgaggg cgaccgcgtc gagatcctct ccggtgtgtt cgaaggccgg
     5101 accaccggta cgccgattgc gctgctgatc cgcaacgaag accagcgcag caaggactac
     5161 ggcaatatcg ccgagacctt ccgccccggc cacgccgact acacttacac gcagaagtac
     5221 ggtttccgtg actatcgcgg cggtggccgt tcgtcggcgc gggagactgc cgtgcgcgtc
     5281 gccgccgcag caatcgcgcg caagtggctg cgggagaacc gcggcatcac cgtgcgcggc
     5341 tggatgagtg cgctgggtcc gatcgaaatt ccgtttgccg acgaagaggc gatcgagcgc
     5401 aatcccttct tcgtgcccga cgagcggatc gtccccgaac tcgagcgcta catggacgaa
     5461 ttgcgcaagg ccggcgattc cgtgggcgca cggcttgacg tgatcgcgcg cggcgtgcca
     5521 ccgggcctcg gcgagcccgt ctacgatcgc ctcgacgccg acatcgcaca cgcgatgatg
     5581 ggcatcaacg cggtcaaggg cgtcgagatc ggcgccggct tcgccagcgt cgcgcaacgt
     5641 ggcagcgaac atggcgatga aatgtcgccg gcaggttttc tctccaatca cgctggcggc
     5701 atcctcggcg gcatttccag cgggcaggac atccgcgtca gcattgcgat caagccgacg
     5761 tcgagcatcc gactggagcg aaaaaccgta gatcgccacg gcaatccagc cgttgttgcc
     5821 acgcatggcc gccacgatcc ctgcgtcggc attcgcgcga cgccgattgt cgaggcgatg
     5881 ctggcgctgg tgctgatgga tcatgtgctc cgccatcgag cccagtgcgg cgatgtgcgc
     5941 tgcgacacgc cggtgatccc gggcgccgta tccggcacgt gaggcggtcc gacacgggcg
     6001 gactcctcag tcgacgccga gggtgttggc aatgagcgca ccgccgctgc cgtattggcg
     6061 gctgtcggcg tactacctcg cgtatttctc ctttctcggc gcctttgcac cgtacttcgg
     6121 cctgtacctc caggaacgcg ggctgtcggc ctggtacatc gccgtcctgc tgtcgcagat
     6181 gcagctgatg cggatcgtcg cgccgattgc ctggggctgg atcgccgacc acagcggccg
     6241 cccggtggcg atcgtccgtg cctcggcggt gctgagcttg gctggaatgg tggctttcct
     6301 cctggtcgat tcggtcgccg gctggttcgt gacgatcgcg gtgatggcct tcttctggag
     6361 cgcggcgctg ccgctggtcg aggtggtgac gatggcacgc ctgggtccga gcggcgccgg
     6421 ctacacgcga attcgcctgt ggggctcgat cggtttcgtc gttgccgtcc tcatgattgg
     6481 tgggctgatc gaggcgatct cgctgcaggt cttgccggtg gcgctgattg cgatgctcat
     6541 cgcggtcgtc ggcagttcgc tggcgctgcc cggcgacggc gtccgcattc ggcccgaagc
     6601 ggcggtacgc atcagcgcgg tcctgcgcca accgcgtatt cgccgcttgc ttggtgcctg
     6661 cttcttcatg tcgcttgcgc acggcgcgct ttacgtcttc tattcgatcc atctggcaga
     6721 tgccggatac ggggcaaccg cgatcggttt cctctggtcg ctcggcgtgc ttgccgaaat
     6781 cgcggtcttt cgctgggggc accgctggca gcatctggcc tcgccgcggg tgctgctggc
     6841 agtctgcctg ctggcggcag tcattcgttt tgcgacgatc ggctggggcg tcgatttcct
     6901 gatcctgttg atcgtcgggc agctgctgca cgggctgacc tttggagcct ttcatgcggt
     6961 ttcggtcgct gccgtccatc ggtggtttcc ggccggcagc cagagccgtg gccaggcgct
     7021 ctattcaagc atctcctttg gcgccggcgg ctttgccggc ggcctgctga gcggcgccat
     7081 gtgggatgcc tggggtgggg ctatgagcta cacggtcagt gccgcgtttg cactggctgg
     7141 catcgtcgtc gtcgtcggac cggcgctggc acgtcgcctg tggcgtcggc gcgctgtcca
     7201 agcctaatcg ccggcgcctg cctatgcgat aatttcaggc tcccaacgcg cgaaaagacc
     7261 atgccgcaaa cgctctacga caaactctgg caaagccacg tcgtccgcca ggaaggcgac
     7321 ggtacggcgc tgctctacat cgatcgccac ctgatccacg aggtaaccag tccgcaggcc
     7381 ttcgaggggc tgaagatcgc cgggcgcaag ccctggcgtg tctcgtcgat cgttgccacg
     7441 gccgaccaca acacgcctac cgatcactgg gagcgtggca tcgaagaccc gatttcacgc
     7501 ttgcaggttg aggcgctcga taccaacatc cgtgctgtcg gtgcgctggc gtacttcccg
     7561 ttccgtgacg cgcgacaggg aatcgtccac gtcgtcggtc ccgagaacgg tgcgacgttg
     7621 ccgggcatga ccgtcgtctg tggtgactcg catacttcga cgcacggtgc cttcgcggcg
     7681 ctggccttcg gcatcggcac ctccgaggtc gagcatgtgc tggcgacgca gtgcctgctg
     7741 tcgaagcagt cgaaatcgat gcgcattcgc gtcgacggca agctcggccg cggcgtcggc
     7801 gccaaggaca tcgcgctcta cgttatcggt gtcatcggca ccgcgggcgg aaccggtcat
     7861 gccatcgagt ttgccggcag cgcgatccgc ggcctgtcga tggaagggcg gatgaccctg
     7921 tgcaacatgg cgatcgaggc cggtgctcgg gccggcatgg tcgcggcaga cgaaacgacg
     7981 atcgcttacc tgaagggtcg ccagttctcg ccgaagggtg ccgactggga tcgcgcggtg
     8041 agctactggc gcacgttgca cagcgacgag ggagcgcgct tcgatcgtga catcagcgtc
     8101 gacgcagcgg cgattcagcc tcaggtcacc tgggggacgt cgcccgaaat ggtggccgct
     8161 atcgacggtc gggttcccga tccggttcgc gagaccgatc cggtgcgtcg cgagggctac
     8221 gagcgggcgc tcaactacat ggggctgacg ccgaacatgg cgatcaagga catcaagatc
     8281 gacaaggttt tcattggctc gtgcaccaat tcgcgaatcg aggacttgcg cgtcgccgca
     8341 gctgtcgcca aggggcgtca ggtcgcggcg aacgtcaagc tggccttagt cgtcccgggg
     8401 tctggcttgg tcaagcaaca ggccgaggcc gaggggctgg acaagatttt cgaggcggct
     8461 ggattcgaat ggcggcagcc gggctgctcg atgtgcctgg cgatgaatgc cgaccggctc
     8521 gagccgggcg agcgttgcgc atcgacctca aatcgcaatt tcgagggacg gcagggtccg
     8581 ggcgggcgca cccatctggt gagtccggcg atggccgctg cggcggcgct ggccggacgt
     8641 ttcgtagacg tgcgggacct gggagtctga ccatgcgcat gctgatgtcg ttagcagtgc
     8701 tggtggcgac gatgacgctc gccggctgca acacctggcg cgggcttggc caggatgtgc
     8761 aacaggttgg tggcgcggtc ggggaaacgg cggagaaggt tggtccgaag atcggcgaga
     8821 ccgccgagaa ggtaggccag gcgatcggtc gcggcgtcga gcgcgtcgga aagagcatca
     8881 agggagctgc cgacagtccg cgcgacgaag tcgatcggcc ccggcaatag gagagatacg
     8941 tggaaaagtt cgttcgcctg gaaggcctgg tcgcacctct cgaccgtaac aacgtcgaca
     9001 ccgacgcgat cattcccaag cagttcctca agtcgattca tcgcacgggt tttggcccca
     9061 actgcttcga tgagttgcgt tatctcgacc acggcgagcc gggaatggat tgcagcaagc
     9121 gaccactgaa tcccgccttc gtgctcaacc agccgcgtta ccgcggcgcc aaggtgctgc
     9181 tgacgcgacg taacttcggc tgcggcagtt cgcgcgagca cgcgccctgg gcattactcg
     9241 actgcggctt ccgcgcaatc ctggccgaga gtttcgccga catcttctac aacaactgtt
     9301 tcaagaacgg catcctgccg atcgttctga gtgccgatca gatcggcgcg ctgttcgcgc
     9361 agagcaccga tggtgaaggc ttgaaggtgg tcgtcgacct gccgtcgcag cgcgttctgc
     9421 tgcccggcgg tgacgcctac ggtttcgacg tcgatccgtt ccgcaaggag tgcctgctaa
     9481 acggctggga cgacatcggg ctgaccttgc gccatgcaga cgagatcagt gccttcgaag
     9541 ccagacgcca gcacgagcag ccttggctgt ttgcctgacc acgtaaagac aacggaacag
     9601 gactcatgaa aatcttggtt ttgcctgggg acggcatcgg ccctgaaatc accgctgagg
     9661 cggttcgcgt gctcgaagtg cttcgtcgcg acggtctcgc catcgaactc gagaacgggc
     9721 tgctcggcgg tgctgccgtc gacgcccatg acgatcccta tcccgaagcg acgcgccggc
     9781 ttgcgcgcgc cgccgatgcc gtgttgcttg gcgcagtcgg tggccccaag tgggatcagc
     9841 tgccgcggcc gaagcggccc gagcgtggtc tgcttggcat ccgcaaggat ctgaatctgt
     9901 ttgccaatct gcggccagca atcctttatc ctgaacttgc gaactcctcg acgctgaagc
     9961 ctgaggtggt tgccggactc gatatcttga tcgtccgaga gctgaccggc gatatctact
    10021 ttggccagcc gcgcggcgtc gaagagcgcg ataccgagtg ggggcggcaa aaggtcggct
    10081 tcaatacgat ggtctatgct gagagcgaga ttcgccgtat cgctagggtt gccttcgagg
    10141 cggcgatgaa gcgcgggcgc aagctctgtt cggtcgacaa gatgaacgtg cttgaatgca
    10201 cgcagttttg gcgcgacatc gtcatcgacg tggcgaagga ttatccgcag gtcgagctct
    10261 cgcacatgct ggtcgacaac gcggcaatgc agttggtgcg tgcgccgaag cagttcgacg
    10321 tgatcgtcac cggcaacatg tttggcgaca tcctgtccga tgaggcgtcg atgctcaccg
    10381 gctcgatcgg catgctgcct tcggcctcgc tcgacgaaaa gaacaagggg ctctacgaac
    10441 cctgccacgg ctcggcgccg gacatcgccg gccagggagt tgccaacccc ctggcaacga
    10501 ttctgtcggc ggcgatgatg ctgcgctaca cctttgcacg cgaggacgct gcgcagcgca
    10561 tcgaggctgc ggtcaagcaa gtgcttgcgc agggattccg taccggcgat atttacgagc
    10621 cgggcaccaa gcgcgtcggg acgcgcgaaa tgggcgatgc ggtcatcgcc gcgctctaag
    10681 aaattcaaac aaggctcgga actgaaatga tgcgagtcgg actggtaggt tggcggggca
    10741 tggtcggctc cgtgctgatg caacggatgc gcgaggagaa tgacttcgcg ttgttcgagc
    10801 cggtgttctt tacgacctcg aacgttggtg gcgcgtcgcc caatttcggc caggaagcgc
    10861 cgccgctgca ggatgccaaa tcgatcgatg cgctcaaggc gatggacgtg atcgtcacct
    10921 gccagggtgg tgactacaca acggagatct atccgaagct gcgcgcggcg ggctggaatg
    10981 gacactggat cgatgcggcc tcgtcgctgc ggatgaagga tgacgcggtg atcatccttg
    11041 atcccgtcaa cctcaaggtc atcaaggacg cgctcgccaa gggcgcccgc aactggattg
    11101 gcggcaactg tacggtttcg ttgatgctga tggccgttgg tgcgctctac cagcgcgacc
    11161 tggtcgagtg gatgagcgca atgacctatc aggccgcttc cggcgccggg gcgcagaaca
    11221 tgcgcgaact gctggcgcag atgggcgaac tcaatcgtgc cgtcaagtcg cttctcgacg
    11281 atccggcctc ggcaatcctc gatatcgacc gcgaagttgc cggcacgctg cgcgccgaag
    11341 gctttccagc cgccaacttt ggcgtgccgc tcgccggttc gctgatcccc tggatcgaca
    11401 aggaactcga gagcgggcag agcaaggagg agtggaaggg tcaggccgaa accaacaaga
    11461 ttctcggtcg cgaagcctcg cccgtgccga tcgacggcat ttgcgtgcgc atcggcgcga
    11521 tgcgttgcca ttcgcaggcg ctaacgatca agctaaagaa agacgtgccg cttgccgaaa
    11581 tcgaagacat gattcgagaa tcgagcgcct gggtgaaggt cgttcccaat catcgtgacg
    11641 tgacgatgcg cgaactgacg ccgaccgccg tgaccggcac gctgacggtt ccggtgggtc
    11701 gcatgcgcaa gctgaatatg gggccggagt acttgggtgc
//
LOCUS       DATDAA010000122         3109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_8762,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000122 DATDAA010000000
VERSION     DATDAA010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8762"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2800)
                     /locus_tag="VIS73_03905"
     CDS             complement(<1..2800)
                     /locus_tag="VIS73_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5762331.1"
                     /translation="MRLPEICIQRPVFASVLSLVVILIGIVSYSRLPVREYPKIDEPV
                     VTVDTTYRGASAEIIESQVTKPLEDSLAGIEGIEVLSSISRAERSQISIRFKLEREPD
                     GAAADVRDRVSRVRQRLPTDVDEPVIAKVEADANPIIWIAFSSDKHSALEVTDVANRI
                     VKPRLQTLSGAADVRIFGDRKYAMRIWLDRERLAGYGLTPQDVEDALRRQNVEVPAGR
                     IESTTREFSVVAKTKLETPAEFEAVVIREASGYPVRIRDVARVEIGPEAERSVVRFNG
                     RPAVAMGVIKQATANPLTLSQALRAELPRVVSELPEGMTARIAYDSSVFIDRSIKAVF
                     TVIGEAVLLVLIIIFFFLRNLRATIIPLVTIPVSLIGAFAVMFVLGFTINTLTLLALV
                     LAIGLVVDDAIVMLENIYRYIEQGMDRMQAAIKGAREIAFAVVAMTITLAAVYAPVAF
                     MTGRTGKLFIEFALTLAGAVLVSGFVALTLSPMMCSLLLKHETRHGRAFMAVEAFLHA
                     MTEGYRRVLRKSLAHRWIVLAAFVVVTAAGGVLLKVLKSELAPVEDRGVIVGVFLGPE
                     GATLEYTDKYARQLEGIYAQTKDVERYFVVAGNPTVSQGISFIGLDDWSVRNRKSSDV
                     AKEFFPRFMGVPGVMAFPVTPPSLGQSPRERPVNFVILSSAPVEEMQSVADQMMGEIA
                     RNPGFITPDTDLKLNKPELSVRVDRDRATDSGIQVETIGRTLETLLGGRPVTRFERDG
                     EQYDVIVQVANADRRNPGDIDQIYVRSNRGEGSGTMVPLSSLVSVDERVAPRELNHFA
                     QRRAITITANLAPGYTMGEALDFLDATAAKLLPAGYATDTNGQTREFRQSSASLALTF
                     ALALAFIYLVLAAQFESFRDPFIIMLTVPLSITGALVALWATGGTLNVYSQIGLVTLI
                     GLITKHGILIVEFANQLQA"
     gene            complement(2807..>3109)
                     /locus_tag="VIS73_03910"
     CDS             complement(2807..>3109)
                     /locus_tag="VIS73_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HEY5762332.1"
                     /translation="LVPEEALVPFGSDQFVFRVDAQGESSKVARVRVETGVRRDGAVE
                     IRSGLAAGDRVVVAGQLKIRDGAAVKPIPSSGANTAAADGGASPAPRAPAAEPGKG"
BASE COUNT          538 a         1044 c         1031 g          496 t
ORIGIN      
        1 tcgcctgcag ctggttggcg aactcgacga tcaggatgcc gtgcttggtg atcaggccga
       61 tcagcgtcac caggccgatc tggctgtaga cgttgagcgt gccgccagtg gcccacagcg
      121 cgaccagcgc gccggtgatc gacagcggca ccgtgagcat gatgataaag gggtcgcgga
      181 agctctcaaa ttgcgcggcg agcaccaggt agatgaaggc cagcgccagc gcgaaggtca
      241 gcgccagcga ggccgacgac tggcggaact cgcgcgtctg gccgttggtg tcggtggcgt
      301 agccagccgg caggagcttc gccgcggtgg cgtcgaggaa gtcgagcgcc tcgcccatcg
      361 tgtatcccgg cgccaggttg gcggtgatgg tgatcgcgcg gcgctgcgca aagtggttga
      421 gttcgcgcgg cgcgacgcgt tcgtccacgc tgaccaggct ggacagcggg accatggtgc
      481 cgctgccctc accccgattg ctgcgcacgt agatctggtc gatgtcgccg gggttgcggc
      541 ggtcggcgtt ggcgacctgg acgatcacgt cgtactgctc gccgtcgcgc tcgaagcgcg
      601 tcaccggccg gcctccgagc agggtctcga gcgtgcggcc gatggtctcg acctggatgc
      661 cggaatcggt ggcgcggtcg cggtcgacgc gcaccgacag ctcaggcttg ttgagcttca
      721 aatcggtatc tggcgtaatg aagccggggt tcctggcgat ctcgcccatc atctggtcgg
      781 caaccgactg catctcctcg accggcgccg aactgaggat gacgaaattg accggccgtt
      841 cgcgcgggct ctgtccgagc gagggcggcg tcaccggaaa ggccatgacg ccagggaccc
      901 ccatgaagcg ggggaagaac tccttggcga catcactcga cttgcgattg cgcacgctcc
      961 agtcatccaa cccgatgaag gagatgccct gcgacaccgt cgggttgccg gcgacgacga
     1021 agtagcgctc gacgtccttc gtctgcgcgt agatcccctc gagctggcgt gcgtacttgt
     1081 cggtgtattc gagcgtcgcg ccttcggggc cgaggaagac accgacgatc acgccgcggt
     1141 cctcgaccgg cgccagctcc gacttgagca ccttcagcag cacgccaccg gcggccgtga
     1201 caacgacgaa ggcggccagc acgatccagc gatgggctag gcttttccgt agcacgcgcc
     1261 ggtagccctc ggtcattgca tgcagaaaag cctcgacggc catgaaggcc ctgccgtgtc
     1321 tcgtctcgtg cttgagcagc agcgaacaca tcatcggcga cagggtcagc gcgacgaagc
     1381 ccgagaccag caccgcgccg gccagcgtca acgcgaattc gatgaagagt ttcccggtgc
     1441 gcccggtcat gaaggcgacc ggcgcgtaga ccgcggccag cgtgatcgtc attgccacta
     1501 cggcgaaggc gatctcgcgc gcacccttga tcgccgcctg catgcggtcc attccctgct
     1561 cgatgtaacg gtagatgttc tcgagcatga cgatcgcgtc gtcgaccacc aggccgatcg
     1621 ccagcaccaa cgccagcagc gtcagcgtgt tgatggtgaa gccgagcacg aacatcacgg
     1681 cgaaggcgcc gatcagcgac actgggatcg tcactagcgg gatgatcgtc gcccgcaggt
     1741 tgcgcaggaa gaagaagatg atgatcagca ccaggagaac cgcctcgccg atcaccgtga
     1801 acaccgcctt gatcgagcga tcgatgaaca ccgaggagtc gtaggcgata cgcgcggtca
     1861 tgccttcggg caattcgctg accacgcgcg gcaactcggc gcgcagcgcc tgcgacaacg
     1921 tcagcgggtt ggccgtggcc tgcttgatga cgcccatcgc caccgccggc cggccattga
     1981 agcgcaccac cgaacgctcg gcctcggggc cgatctcgac gcgcgccacg tcacggatgc
     2041 gtaccgggta gcccgaagcc tcgcggatga ccaccgcctc gaactcggcc ggcgtctcca
     2101 acttggtctt agccaccacc gagaactcgc gggtcgtgct ctcgatgcgc ccggccggca
     2161 cctcgacgtt ctgccgtcgc aaggcgtcct cgacgtcctg cggcgtcagc ccgtagccgg
     2221 ccagccgctc gcggtcgagc cagatgcgca tcgcgtactt gcggtcgccg aagatgcgca
     2281 cgtcggcggc gcccgacagc gtctgcaggc gcggcttgac gatgcggttc gcgacgtcgg
     2341 tgacctccag cgccgaatgc ttgtcgctgg aaaaggcgat ccagatgatc gggttggcgt
     2401 cggcctcgac cttggcgatc accggctcgt cgacgtcggt gggcagccgc tggcgcacgc
     2461 gcgagacgcg gtcgcgcacg tcggcggcgg cgccgtcggg ctcgcgctct agcttaaagc
     2521 gaatgctgat ctggctgcgc tcggcgcgcg agatcgacga cagcacctcg atgccctcga
     2581 tacctgccaa cgaatcctcg agcggcttgg ttacctgaga ctcgatgatc tcggcgctgg
     2641 cgccacggta agtcgtatcg acggtgacca ccggctcgtc gatcttcgga tactcgcgta
     2701 ccggcagccg cgagtaggac acgatgccga tcaggatcac taccagcgac agcacgctgg
     2761 cgaagaccgg ccgctggatg cagatttccg gcaggcgcat ggctgctcag cccttgccgg
     2821 gttcggcggc cggcgcacga ggcgccggac tggcgccgcc gtccgccgcc gctgtgttgg
     2881 ctccgctaga cgggatgggt ttcaccgccg cgccatcacg aatcttcagc tgcccggcga
     2941 ccaccacgcg atcgccggcc gccaaccccg agcggatttc cacagcacca tcgcggcgca
     3001 cgccggtctc gacgcgcacg cgcgccacct tgctcgactc gccctgcgca tcgacgcgga
     3061 aaacgaactg gtcgctgcca aagggcacca gcgcctcctc gggaaccag
//
LOCUS       DATDAA010000123         2065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_498283,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000123 DATDAA010000000
VERSION     DATDAA010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498283"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..364
                     /gene="rpsT"
                     /locus_tag="VIS73_03915"
     CDS             98..364
                     /gene="rpsT"
                     /locus_tag="VIS73_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY5762333.1"
                     /translation="MANSAQARKRARQSEKQRAHNASLRSTLRTAVKKVRKAIASGDQ
                     AAAKAVFQESQSVIDRIADKKIVHKNSTSRTKSRLSAAIKAIAA"
     gene            complement(444..773)
                     /locus_tag="VIS73_03920"
     CDS             complement(444..773)
                     /locus_tag="VIS73_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HEY5762334.1"
                     /translation="MTEPQTASFIIVGRTREGSKFRPSDWAERLCGVMSAFGAEKRMK
                     YSPLVRPCTFDSEKSVFIDGQLSTLEPLAYKFMLNFATDNDLRVVEQEAAFCEIGENG
                     EINCKSV"
     gene            1036..>2065
                     /locus_tag="VIS73_03925"
     CDS             1036..>2065
                     /locus_tag="VIS73_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY5762335.1"
                     /translation="MAHLMNTYARLPVALVGGKGCRVVDDAGRSYLDALAGIAVSTLG
                     HAHPDLIAAISDQAGRILHHSNLYRAPLQEKLADRLCALAGMDEVFFGNSGCEANEAA
                     IKLARFYGHQRGIRQPAIVVMERAFHGRTLATLSATGNRKAQAGFEPLVSGFVRVPYN
                     DMQALREVAAHNGDVVAVMLEMIQGEGGIHLADEDFQRDLRALCDAKGWLLICDEIQC
                     GMARTGRWFGFQHAGIRPDIMTLAKGLGSGVPIGACVTAGPAAGLFKPGNHGSTFGGN
                     PLACRAALTTLDVIERDGLLGRAAIAGQRIRDGLAKALDGQAGLVAIRGQGLMIGVEL
                     DRACGELVT"
BASE COUNT          375 a          635 c          679 g          376 t
ORIGIN      
        1 ccggaagctt ccgcgggcct cgcagaaggc ttgcgcattt cgattcaagc cgttagaatc
       61 cgcgccctta tccaactacc cagcaggcag gcagacaatg gccaacagcg cacaagcacg
      121 caaacgcgct cgtcaatccg agaagcaacg cgcccataac gccagcttgc ggtcgacgtt
      181 gcgtacagcg gtgaagaaag tgcgcaaggc gattgcaagc ggtgaccagg ccgccgccaa
      241 ggcggtgttc caggaatcgc agtcggtaat cgatcgcatc gccgacaaga agatcgtgca
      301 caagaattcg acttcgcgga ccaagagccg tctttcggcc gcgatcaagg caatcgccgc
      361 ctgatcgcga ttcacgggcg agaaaaacgg cgcctcgggc gccgttttgc tttggggtgt
      421 ttggcgtgaa tcgcctggcg cgatcagacc gacttgcagt tgatctcgcc gttctcgccg
      481 atttcgcaga acgccgcttc ctgctcgacc acgcgcagat cgttatcggt cgcaaaattg
      541 agcatgaact tgtaggccag cggttccagc gtcgacaatt gcccgtcgat gaacacggac
      601 ttttcgctgt cgaaggtgca ggggcggacc agcggcgagt acttcatgcg cttttcggca
      661 ccaaacgccg acatcacgcc gcacagccgc tctgcccagt cgcttgggcg aaacttgctg
      721 ccctcacggg tgcggccgac aatgatgaaa ctggcggttt ggggttcggt catgatgatg
      781 ccggtttgcg gcgtacgtga agcggaggcg ccggaagata gggcgggatg ccgcccccga
      841 cgcggcgcga agtttagcag agattgcttt gaccaacgag ccccggaagg gcgcggaccg
      901 gagcgaagcg cgcgcagccg ggagcacgat gacagcgcaa gccttttgtc ttagtattcc
      961 ctccgcgata cgggcacggc ggctttggcc gccgttctgt ttttctcgta ccaacagctt
     1021 tgtgtggatg aagccatggc tcacctgatg aatacctatg cgcgcctgcc ggttgccctc
     1081 gttggtggca agggctgccg cgttgtcgac gatgcaggcc gcagttacct cgatgcgctg
     1141 gccggcatcg cggtctcgac gcttggtcat gctcacccgg acctgatcgc ggcgatcagc
     1201 gatcaggcgg ggcgcatcct gcatcattcg aacctctacc gcgcgccgct ccaggagaag
     1261 ctcgccgacc gcctgtgcgc gcttgccggg atggacgagg tctttttcgg caattccggt
     1321 tgcgaggcga acgaggcggc gatcaagctg gcgcgcttct acggccatca gcgcggcatc
     1381 cgccagccgg ccatcgtcgt gatggagcgc gctttccacg gccggacgct ggcaacgctt
     1441 tcggcaaccg gcaaccgcaa ggcacaggcc ggattcgagc cgctggtatc gggcttcgtc
     1501 cgtgttccct acaacgacat gcaggcactg cgcgaggtcg ctgcgcacaa cggcgacgtc
     1561 gtcgcggtca tgctagagat gattcaggga gaaggtggca tccaccttgc cgacgaggat
     1621 ttccagcgcg acctgcgcgc gctgtgcgat gccaagggct ggctgctgat ctgcgacgag
     1681 attcagtgcg gaatggcgcg caccggtcgc tggttcggtt ttcagcacgc cggaatccgg
     1741 cctgacatca tgacgctggc caagggactc ggttcgggcg tgccaatcgg cgcctgcgtc
     1801 accgcaggac cggcggccgg cctgttcaag ccgggtaacc atgggtctac gtttggcggc
     1861 aatccgctgg cgtgtcgcgc ggcgctgacg acgctggacg taatcgagcg cgacgggctg
     1921 ctcggccggg cggcgattgc cggccagcgg attcgcgacg gtctggcaaa ggcgctcgat
     1981 gggcaggctg gcctggtggc gattcgcggc cagggactga tgatcggcgt cgaactcgac
     2041 cgcgcctgcg gcgaactggt cacac
//
LOCUS       DATDAA010000124        16548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_114303,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000124 DATDAA010000000
VERSION     DATDAA010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 16548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16548
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114303"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VIS73_03930"
     CDS             <1..241
                     /locus_tag="VIS73_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762336.1"
                     /translation="IVEVAEVATPPSGSDYFKTVLANAIATGALSGDYTSKAEGFEIR
                     SQRTGAADAQGCVQVETTISNDLLLAGNRVTRACP"
     gene            325..2238
                     /locus_tag="VIS73_03935"
     CDS             325..2238
                     /locus_tag="VIS73_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5762337.1"
                     /translation="MPLVSLANASIAYGDLPLLDGVAFQVDPGERVAVVGRNGSGKST
                     LLKVLAGEQALDGGENWRQPGLSVGYVPQEPDFDPEQTVFASVVSGMGDVSRLVAEFH
                     DVSHQIAEQQGSEADLLSRLHDVQSELDARGAWRYAAQAEQVISRFGLDPDQRVGVLS
                     GGQLRRVALARALATSPDLLLLDEPTNHLDIAAISWLEEFLLGLPTAIIFITHDRRFL
                     DRVASRVIELDRGRLASFPGSFSAYRKRKDELLHAESLAEARFDKRLKEEEIWIRKGV
                     EARRTRAVFRVQRLDQLRAARAARREQLGVAKLRLERGGESGELVAELEGVSKAFGSL
                     TVVRDFSCRIIRGDRIGLVGPNGAGKTTLIRLILGELEADSGRIRLGTRLAVAYSDQL
                     RDTLDPAATLAETISPGSEFVEIGGKRQHVIGYLGDFLFSPQRARSPVSSLSGGERNR
                     LLLARLFARPANVLVLDEPTNDLDIESLELLESLLQDYTGTILLVSHDRAFLDNVVTQ
                     VIGAEGEGRWCENAGGYDDWVAFQAARAQQAAEQPAGRTVAAAPKGGAERARSRKALS
                     YKEEKELEGLPAAIDKLETEQGSIAAQLGDPALHSERPSDARRLAERLAAIEDQLTKL
                     LERWEELEAKRDA"
     gene            2316..2612
                     /locus_tag="VIS73_03940"
     CDS             2316..2612
                     /locus_tag="VIS73_03940"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY5762338.1"
                     /translation="MIESATRLGGDEIANRLTGLGGWALRDGALQKTFSFGNYHKTMA
                     FVNAVAWIAHRMDHHPDLLVGYNRCVVRYATHSAGGITPLDFEAAAAVESLGSL"
     gene            2671..3624
                     /locus_tag="VIS73_03945"
     CDS             2671..3624
                     /locus_tag="VIS73_03945"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HEY5762339.1"
                     /translation="MEGLADDVLREILTGVGGFDWCVSEFARVSSTVLPARFFRRIAP
                     ELDHGARTQAGVPVRVQLLGADPARMAGSAQRLAQLDPWGIDLNFGCPTPTVNRHRGG
                     ACLLDEPDLLFSIAAAVRQAVPVTMVLSAKMRLGVSDPSRAVEVAQALVAAGVGELAV
                     HARTRADMYRHPARWEWIGRIREAVAVPVVANGDIRSVADWQRCRAITGCDAAMLGRG
                     AVADPLLARRLKGSHPAAVTADDWPLIAPLLADFWVRVQEKLAPRHAPGRIKHWLSLL
                     AGAHPEAARLYAELRPLSDVAAIGRLLDAALAPSARRRAAA"
     gene            3621..4322
                     /locus_tag="VIS73_03950"
     CDS             3621..4322
                     /locus_tag="VIS73_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5762340.1"
                     /translation="MSRAGPPDQPAVFPAVGNSRVVSSAQTGIHRDLPRLLARHRDHA
                     FQRPIGAVSRLAVEAALAQRAGRPLILDSGCGVGQSTVKLARRFDDSFVLGIDQSAAR
                     LARAPELPDNACLIRANLVDAWRVLAARGERLARHYLLYPNPWPKIGHLARRWHGHAV
                     FPELLALAGVLECRSNWAVYVAELAAAVTVLTGRAAVPEELPADLALADPLTPFERKY
                     AASGQTLYRLVVDLG"
     gene            4411..5823
                     /locus_tag="VIS73_03955"
     CDS             4411..5823
                     /locus_tag="VIS73_03955"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004939.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY5762341.1"
                     /translation="MSEQLDVIIVGAGSAGLAALREVRKATERFVIINDGPYGTTCAR
                     VGCMPSKALIEAANAFHARHKHEAFGIHGGELLAIDVPAVLRRVRGLRDRFVGGTLKA
                     TDELGERSIAGRAVLLGPDRLRVGDRELRAKRIILAPGSRPIVPEAWRALGARIQTTD
                     SLFELEDLPPRMAVIGLGAIGIEMAQAISRLGVEVAAFGRSPSLAGLTDEKVNAVLRE
                     RLDRELAIHVGADVELQAAGDEVLVRSGSVEVHVDQVLVAIGRRPNLDEIGLDSLGVP
                     LDSRGMPDFDQTTGRIGELPVFIAGDANGHVALLHEAADEGYIAGINATAVTPICFRR
                     RTPIGIVFADPGVAFVGRRLAELDGVQIVIGEVSYAKQGRARTAERNYGLLRVYADRE
                     DGRLLGAEMAVPAAEHLAHLLALAIDRELDVHALLGMPFYHPVLEEGLRTALRDAARQ
                     LPTRRSDLAACEAFRAEALD"
     gene            complement(5840..6214)
                     /locus_tag="VIS73_03960"
     CDS             complement(5840..6214)
                     /locus_tag="VIS73_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HEY5762342.1"
                     /translation="MTCPCGSNRPLASCCGRFHAGEAAPDPETLMRSRYSAFVGKRAD
                     YLLTTWHPRTRPAHLSLDEEPAPKWLGLTIKRAESAGDTGLVEFVARHRIGGRAFRLH
                     ETSRFERVDGRWYYLDGELHSR"
     gene            complement(6280..6540)
                     /locus_tag="VIS73_03965"
     CDS             complement(6280..6540)
                     /locus_tag="VIS73_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762343.1"
                     /translation="MNRKWTLDRIEFDNETYEIIRPEASDAPAPAAEHPQRALQLGLS
                     LAADQRFSRQSRIPEALIAATMDIDEPVFRDPDQPSSTRQVH"
     gene            complement(6783..7346)
                     /locus_tag="VIS73_03970"
     CDS             complement(6783..7346)
                     /locus_tag="VIS73_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5762344.1"
                     /translation="MSDTAHRAHRGGDASPWVMRWAASLPAGARVLDLACGSGRNARW
                     LAAQGFVVRAVDRDAEALAGLAGVPGISTRQADLEGAPWPLVGETFDAVVVCRYLHRP
                     LFPQILATVTPGGLLIYETFMLGNEQYGRPSRPEFLLAPGELAALANKAGWAIEGLRE
                     GVESIDAADASHKAVTQSICARKPVTA"
     gene            complement(7343..8443)
                     /gene="amrS"
                     /locus_tag="VIS73_03975"
     CDS             complement(7343..8443)
                     /gene="amrS"
                     /locus_tag="VIS73_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEY5762345.1"
                     /translation="MSEARVLPDSGNTAAQWWHRLDDGRVQCDLCPRDCRLHEGQRGA
                     CFVRAYDGQQMVLTTDGRSSGFCIDPVEKKPLNHFYPGSSILSFGTAGCNLACKFCQN
                     WDISKSRDMDRLMDQASPEAIARAAHAAGARSVAFTYNDPVIFYEYAIDVAEACHAIG
                     IDTVAVTAGYIHALPREPFFHHMDAANVDLKAFTDEFYVRQCGARLQPVLDTLAWIWH
                     ETNCWLEITTLLIPGLNDSETEIAELSAWVARELGPEVPLHFTAFHPDWKLTDRPATP
                     AATLTRARRQALDAGLRHVYTGNVRDLAGGTTHCTGCARALIVRDGYAIRDYRLRDDG
                     TCPDCGTRLAGRFAHYTGSFGNRRIPVRIAAA"
     gene            complement(8436..9029)
                     /gene="amrA"
                     /locus_tag="VIS73_03980"
     CDS             complement(8436..9029)
                     /gene="amrA"
                     /locus_tag="VIS73_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEY5762346.1"
                     /translation="MPAEAARDPGRALLAVARHAIAERLGANTDPDELAAARAALPAG
                     EGASFVTLTRAGALRGCIGTLEARRPLITDVAENALAAAFRDPRFPPLRREEWPGTRV
                     EVSLLAPPEPFVVASEAAAIAALRPGTDGLIFDYGGRRATFLPQVWESLPAPADFLAR
                     LKEKAGYPPDFWHPDVRLSRYGVRKWCEDDDEMARHE"
     gene            complement(9019..9846)
                     /gene="amrB"
                     /locus_tag="VIS73_03985"
     CDS             complement(9019..9846)
                     /gene="amrB"
                     /locus_tag="VIS73_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEY5762347.1"
                     /translation="MTIDPRAGTRPAAVAGTFYPAHEGPLRAAIADAYRAAPVPEALP
                     PSRPRAVVVPHAGYVYSGAIAATAYRTLEPFAAGIRRVVLLGPAHRVAFAGMAVPQVA
                     RFASPLGAIAIDTAARDKALALPGIIASDQPHVQEHSLEVQLPFLQTLLGKFSLLPVV
                     VGEAPPALVGPLIEAFCDEPDTLLLISSDLSHFHRYDEARRIDRETIDRILALQSGLL
                     GEQACGARPINGLLRVAAARGWQARLLAHCNSGDTAGDRQRVVGYAAIGFYDDDHAC"
     gene            complement(9843..11123)
                     /locus_tag="VIS73_03990"
     CDS             complement(9843..11123)
                     /locus_tag="VIS73_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEY5762348.1"
                     /translation="MRDALLTEVGLEFLQVFSLSLGIGLLMGLERERNPAARAGLRTF
                     GLVALFGTVAALLGEKTGSPWMLAVGLLLVGVMTISAYVTHPDPQDPGTTTVVAVLLC
                     FVYGAMVWHGFRIYAVMLSIVTTMLLYFKAELRLFSQKLTRKDLISILQFGVLSLVIL
                     PILPNSDMGPYDALNPAQLWWMVVLISGVSLAGYAALRIVGQHHGAPILGVLGGLASS
                     TATTLVFSRHAASGTHLLRLATVVILLANLVVLVRLSIYGAVLQPTLLPILLPPLAIG
                     LVLGAGFVVAIWRGLNSREDVPALELTNPAEIRTALTFGAVYGLVLVISAALSDYAGS
                     KGLYLVAVVSGLTDVDAITLSSLRLYGLGKLSASDAVTAILIATLSNLVFKLGIVSMV
                     GGALLTRRVAAGFGLIATGLVAGAVWFVLSGAAT"
     gene            11189..11896
                     /gene="ispD"
                     /locus_tag="VIS73_03995"
     CDS             11189..11896
                     /gene="ispD"
                     /locus_tag="VIS73_03995"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEY5762349.1"
                     /translation="MPRYCAIVPAAGSGARFGAETPKQYLSVGNHPLLYHALRPLLRQ
                     PQIERVVVVLSPADRFWQHYEWDDPAGKLMVIREGGASRADSVRAGLAAVQANLHESD
                     WVLVHDAARACLASADLDSLITELTDDPVGGLLAVPLADTLKHGDAAGRVDRTPPRDG
                     LWQAQTPQMFRAGILGEALRAAKAVTDEASAVEALGLKPRLVAAQYPNFKVTYAADLD
                     LARLILEARERVEVVHA"
     gene            11889..12389
                     /gene="ispF"
                     /locus_tag="VIS73_04000"
     CDS             11889..12389
                     /gene="ispF"
                     /locus_tag="VIS73_04000"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY5762350.1"
                     /translation="MPDPMATLPFRVGQGYDVHALVPGRPLIVGGVRIESPVGLLGHS
                     DADVLLHAITDALLGGAGLGDIGRHFPDTDAQFAGADSRVLLRATRGKLAEAGWRVGN
                     LDATVVAQKPRLAAYIPAMCVAIAEDLGIAVGAVNVKAKTHERLGFEGRGEGIAAQAV
                     ALLLPR"
     gene            12386..12982
                     /gene="thpR"
                     /locus_tag="VIS73_04005"
     CDS             12386..12982
                     /gene="thpR"
                     /locus_tag="VIS73_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY5762351.1"
                     /translation="MTRPGTDAVAVDRHRLFFAVWPDPVTAATLADEAQRLAPRLEGR
                     AMRAETLHLTLAFLGNRSGAQRDRLCEGLDQANRSGWPGGFALRLDRLQQWRHNGIVL
                     AASSERCEPLHRLAGLVSELSRTCGIHVPERPFVPHLTLLRSCRGELPARTGGDGPWH
                     WAVRAFRLVESFLGPAGASYRVIGEWALPSAPRADGGG"
     gene            12984..15566
                     /locus_tag="VIS73_04010"
     CDS             12984..15566
                     /locus_tag="VIS73_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5762352.1"
                     /translation="MNRPFLPLAPTGLKISGQEATEIASFVLIANAPAGDRAAISSSV
                     EAHAGRQFFLRYTDEIAGLREALECCSWDAVVLDCRGGSVDFDAYLHVIRHYWPNANI
                     VVWGGEQAGQPVEDRRLLRVTERAPAALVASLADLPRTSPESATPARRSRKEADAAAH
                     LQQLADNIPECLWLQDLVEREVVYVNPAYASITGFEPAMPVNDWRALFDCVYVDDVAR
                     FAEAQARAPFGGLDEEFRIRRADGELRWVHLRTFAIRDREGLIAGVGGAMRDISAAVA
                     QQKHLYQVTHFDGLTALPNRMLLQERLTASVAMARRNDWLLGLLLIDIDRFKIINDTL
                     GHAVGDELLQQAAMRLRACLRDSDTVGRMGGDEFAVVLPDMDSAEMAAIVARKVVEVI
                     AEPFVIDGQELFVSASVGISLYPDDAADIDVLVRNADAAMYRAKDSGRNAYAFYTAEM
                     NVRARRRLGLETDLRQALARDEFELHYQPKVNCRSGAIAGVEALIRWRHPERGLVGPD
                     EFIPLLEETGLIVPVGDWVMRTACAQMQEWHTAGFAQLDIAVNVSARQMEGRRLIEAV
                     RVALDATGLPAAALEIELTESLLMRDPPAVVDMLSELKAMGLQVAVDDFGTGYSSLSY
                     LKRFPLDNLKVDRAFVQDIAASAGDASITRAVIHMGHELALKVVAEGVETAEQLEALQ
                     IAGCDEIQGYYFSRPLPPKDILELLRSGRTLPVRSGADGEYEQRSGLDPAIARALSDL
                     RQERDRQAAAADAARLLWRQTLEAFAEPVVGVDRAGQVVFANDAADEAFAECGLHPGV
                     VIADCLPPLALALGGDASVAVTGADGTRYRVRISRLQARQPSAAGDRSGATLLWFSRD
                     GEVS"
     gene            15563..15790
                     /locus_tag="VIS73_04015"
     CDS             15563..15790
                     /locus_tag="VIS73_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762353.1"
                     /translation="MIDFGSRQSGELLVPVAAGEWRRRRRDQPMRCDPLALARRRQSS
                     RALRQALGGISAATLIRCAMSAGITGEQQEW"
     gene            15784..>16548
                     /locus_tag="VIS73_04020"
     CDS             15784..>16548
                     /locus_tag="VIS73_04020"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY5762354.1"
                     /translation="MVKLSAESVANSLHQLPALHPVVVKLLESFEKAGVDTQSLVHEI
                     AQDQALVTRVLRLANSSFYGLPSHVDSLKDAVMILGFRTVRAVAVAVSLTDCFSSRKV
                     PGFDTNAFWQHSAAVGMAAREIAAIVKRPTDVAFTAGIVHDIGVIALLWLHPKDMAAV
                     LAACRDNGLPLDVAEREVFGVDHCAIGAALARHWNLPEALVESIASHEQPANAEAGSL
                     ADIVHVANAIIHDYGLPAIEVITVSAVSDLAMNRLGI"
BASE COUNT         2646 a         5426 c         5733 g         2743 t
ORIGIN      
        1 gatcgtcgag gttgccgagg tggcgacgcc gccaagtggc tcggactact tcaagactgt
       61 gctcgccaac gcgatcgcga ccggggcgct ctcgggcgat tacacgtcaa aggccgaagg
      121 ctttgagatt cgcagccagc ggaccggtgc ggccgatgcc cagggctgcg tgcaggttga
      181 aaccacgatc agcaacgatc tgctgctcgc tggcaatcgc gtcacccgcg cctgtccctg
      241 agataatccg cgccttcgaa cgcgccgggc acctcgtccg gcggtgcggc ccgccgcgcc
      301 agccgttctc gccgggttct tctgatgccg cttgtttccc ttgccaatgc ctcgattgcc
      361 tatggcgatc tgccgctgct tgacggcgtt gccttccagg tcgacccggg cgaacgcgtc
      421 gccgtcgttg gccgcaacgg cagcggcaag tcgacgctgc tgaaggtcct cgccggagag
      481 caggccctcg atggtggcga gaactggcgc cagccgggcc tgagcgttgg ctacgtgccg
      541 caggaacccg acttcgaccc cgagcagacg gtgttcgctt cggtcgtttc gggtatgggc
      601 gacgtttccc ggcttgttgc ggaattccac gatgtgtcgc accagatcgc cgagcagcaa
      661 ggctccgagg ctgatctgct ctccagattg cacgatgtgc aatcggaact cgacgcccgt
      721 ggagcctggc gctacgcggc gcaggccgag caggtgatct cgcgcttcgg cctcgacccc
      781 gaccagcggg ttggcgtgct ctcgggcggg caactgcgcc gggtggcgct ggcgcgcgcg
      841 ctggcgacct cgcccgacct cctgctgctc gacgagccga ccaaccacct ggacatcgcg
      901 gcgatcagct ggctcgagga attcctgctt ggtctgccga cggcgatcat cttcatcacc
      961 cacgaccggc gcttccttga tcgcgtcgcc agccgtgtca tcgaactcga tcgcggccgg
     1021 ctggcgagtt ttcccggtag cttctcggcc taccggaaac gcaaggacga gttgctgcac
     1081 gccgagtcgt tggccgaggc gcgcttcgac aagcggctca aggaagaaga gatctggatt
     1141 cgcaagggcg ttgaggcgcg gcgcacgcgc gccgtgttcc gcgtccagcg gctcgaccag
     1201 ttgcgtgcgg cgcgggcagc tcggcgcgag cagctcggcg tcgccaagct gcggctcgaa
     1261 cgcggcggcg agagcggcga actggtcgcc gaactcgagg gcgtgagcaa ggcctttggt
     1321 tcgctcaccg tcgttcgcga tttctcctgc cgcatcatcc gcggcgaccg catcggcctc
     1381 gtcggcccca acggcgccgg caagaccacg ctgattcgcc tgattctcgg cgagctcgaa
     1441 gccgattccg gccgcattcg tctcggcact cggctggccg tcgcctattc cgaccagctg
     1501 cgcgacacgc tcgatccggc tgcgacgctg gccgagacga tctcgccggg gtcggagttc
     1561 gtcgagatcg gcggcaagcg acagcacgtg atcggctacc tcggcgactt cctgttctca
     1621 ccgcagcgcg cccgttcgcc ggtcagcagc ctttcgggcg gcgaacggaa ccggctgttg
     1681 ctggcccggc tgtttgcccg gccggccaat gtgctggttc tcgacgagcc gaccaacgac
     1741 ctcgacatcg agtcgctcga actgctcgag tcgctgctgc aggactacac cgggacgatc
     1801 ctgctggtca gtcacgatcg cgccttcctc gacaacgtgg tgacgcaggt gatcggcgcc
     1861 gaaggcgagg ggcgttggtg cgagaacgcc ggcggctacg atgactgggt agccttccag
     1921 gccgcccggg cgcagcaggc cgcagaacaa cctgccggtc ggacggtggc cgccgcgccg
     1981 aagggcggtg ccgaacgcgc ccgttcgcgc aaggcgctga gctacaagga agaaaaggaa
     2041 cttgaaggcc tgccggccgc gatcgacaag ctcgaaaccg agcagggcag cattgcggcg
     2101 cagctcggcg atccggcact gcacagcgaa cggccctccg atgcgcgtcg cctcgccgaa
     2161 cgtctggctg ccatcgagga ccaactgacg aagctcctcg agcgctggga ggaacttgaa
     2221 gcaaaacgcg acgcctgagc gcagcaggcg acaatcacga taatgagtcg attcgcgagt
     2281 cgattcgacc aggcccgcat ccaccggaga tcaccatgat tgagtccgcg acccgcctcg
     2341 gcggcgacga aatcgccaac cgcctcaccg gcctcggtgg ctgggcgttg cgcgacggcg
     2401 ccctgcagaa gaccttttcc ttcggcaact atcacaagac catggccttc gtgaatgccg
     2461 tcgcctggat cgcccatcgc atggaccacc acccggacct gctggtcggc tacaaccgct
     2521 gcgttgtccg ttacgcgacg cattcggctg gtggcatcac gccgctggat ttcgaggccg
     2581 cagcggcggt cgagtcactc ggttcactct gagggcgggc caatcaccaa cgctgtttcg
     2641 gctgcttcgc cacgcgtcgc gctggccccg atggagggtc tggccgacga cgtgctgcgc
     2701 gagatactca ccggcgtcgg cgggttcgac tggtgcgtat ccgaattcgc gcgggtgtct
     2761 tcgacggttc tgccggcgcg cttcttccgc cggatcgcgc ctgaactcga ccacggcgcg
     2821 cgtacccagg ccggggtgcc ggtgcgcgtg cagctgctcg gcgccgatcc ggcgcggatg
     2881 gccggctcgg cacagcggct ggcgcagctc gatccctggg gaatagatct taacttcggt
     2941 tgcccgacgc ctaccgtcaa ccgccaccgc ggcggcgcct gcctgctaga cgaaccggat
     3001 ctgctgtttt cgattgcggc tgccgtgcgc caggcggtac cggtgacgat ggtgctcagc
     3061 gccaagatgc ggctcggcgt cagcgatccc tcgcgcgctg ttgaagtggc gcaggcgctg
     3121 gtcgccgccg gcgtcggcga gctagctgtg cacgcgcgaa cccgcgccga catgtaccgc
     3181 cacccggcgc gctgggagtg gatcggccgc atccgcgagg cggtcgccgt tcccgtggtc
     3241 gccaacggcg acatccgctc ggtcgccgac tggcagcgct gccgcgccat caccggctgc
     3301 gatgcggcga tgctcggccg tggcgccgta gccgatccgt tgctcgcccg ccgcctcaag
     3361 ggcagccatc cggccgcggt gacggccgac gactggccgc tgatcgcgcc cttgctcgcc
     3421 gatttctggg tccgcgtgca agaaaagctg gcaccgcgcc acgcgccggg gcgaatcaag
     3481 cactggctgt cgcttcttgc cggtgcccat cccgaggcgg cgcggctcta tgccgaactg
     3541 cggcccttaa gcgatgtggc ggccatcggc cggctgctcg acgccgcgct cgcgccgtcg
     3601 gcgcggcgac gcgcagcagc atgagcaggg ctgggccgcc cgaccaaccg gctgtattcc
     3661 cggccgtcgg caactcgcgg gtcgtatcca gtgcgcagac cggcatccac cgcgatctac
     3721 cccgcctcct tgcccggcat cgcgaccacg cgtttcagcg gccgatcggc gccgtcagcc
     3781 gcctggcagt cgaggccgca ctggcgcaac gtgccgggcg gccgttgatc ctcgattcag
     3841 gctgcggcgt ggggcagagc accgtgaaac tggcgcgtcg ctttgacgat tccttcgtgc
     3901 tcggcatcga ccagtccgcg gcgagactgg cgcgcgcgcc cgaactgccg gacaacgcct
     3961 gcttgattcg cgccaacctg gtggacgcct ggcgggtgct ggcagcccgc ggcgaacggc
     4021 tggcgcgcca ctatctgctc tacccgaatc catggccgaa gatcggccac ctcgcgcggc
     4081 gctggcacgg ccacgcggtg ttcccggagc tgctggcgct agcaggcgtg ctcgaatgcc
     4141 gcagcaactg ggcggtctac gtcgccgaac tggcagctgc cgtcaccgtg ctgacgggtc
     4201 gcgcggcggt gcccgaggaa ttgccggccg acctggcgct ggccgatccg ctgacacctt
     4261 tcgagcgcaa gtacgcggcc tccgggcaga cgttgtatcg gctcgtcgtc gacctcggct
     4321 gagcgagcgc cgaaccaaag ccgcaccgtc ctgaccaagc aaggcggctt cggcacaatg
     4381 gccgcacaga ctgcaagcga ggagaggcga atgtccgaac aactcgatgt catcatcgtc
     4441 ggcgccggtt cggccggact ggccgcgctg cgcgaggtac gcaaggcgac cgagcgcttc
     4501 gtcatcatca acgacggccc ctacggcacc acctgtgcgc gcgtcggctg catgccgtcg
     4561 aaggcgctga tcgaggccgc caatgccttt cacgcacggc acaagcatga agccttcggc
     4621 atccacggcg gcgagttgct ggcaattgac gtgccagcgg tgctgcgtcg cgttcgcggc
     4681 ctgcgcgacc gtttcgtcgg cggcacgctc aaggccaccg acgaactcgg cgagcgcagc
     4741 atagctggcc gcgccgtgct gctcggcccg gatcgcctgc gagtcggcga ccgtgagctg
     4801 agggcgaagc ggatcatcct cgcccccggc tcccggccga ttgttcccga agcctggcgc
     4861 gcgctcggtg cgcgtatcca gaccaccgac agcctgttcg aactggaaga cctaccgcca
     4921 cggatggccg tcatcggcct cggcgcaatc ggcatcgaga tggcgcaggc gatttcgcgg
     4981 ctcggtgtcg aggtcgctgc cttcgggcgc agcccgtcgc ttgccggtct caccgacgag
     5041 aaggtgaatg cggtgctgcg cgagcgtctc gatcgcgagt tggcaatcca cgtcggcgcc
     5101 gacgtcgagc tgcaagcggc cggcgatgaa gtcctcgtcc gcagcggcag cgtcgaagtc
     5161 cacgtagatc aagtgctggt cgccatcggc cggcggccca acctcgacga aatcgggcta
     5221 gacagccttg gcgtgccgct cgattcacgc ggcatgccgg acttcgatca gaccaccggc
     5281 cgcatcggcg aactgccggt gttcatcgcc ggcgacgcca acgggcacgt cgcgctgctg
     5341 catgaggcgg ccgacgaggg ctacatcgcc ggcatcaacg cgactgccgt cacacccatt
     5401 tgctttcggc ggcgcacgcc gatcggcatc gttttcgccg atccgggcgt ggcctttgtt
     5461 ggccggcgtc ttgccgaact cgacggggtg cagatcgtca tcggcgaggt cagctacgcg
     5521 aagcagggaa gggcgcggac ggccgagcgc aactacgggc tgctgcgcgt ttacgccgat
     5581 cgcgaggatg gccgcctgct tggcgccgaa atggccgtcc ccgccgccga gcacctcgcg
     5641 cacctactgg cgctggcgat cgaccgcgaa ctcgacgttc acgcgctgct cggcatgccc
     5701 ttctaccatc cggtactcga agaggggttg cgcacggcgc tgcgcgatgc cgcgcgccag
     5761 ctgccgaccc gtcgctccga tcttgccgcc tgcgaagcct tccgcgccga ggcgctcgac
     5821 taggcggctg ccgggcgcgt cagcgcgaat gaagctcgcc gtcgaggtag taccagcggc
     5881 cgtcgacgcg ttcgaagcgg ctcgtctcgt ggagccggaa ggcacgaccg ccgattcggt
     5941 ggcgcgccac aaactcgacc aggccggtat cgccggcgct ttcggcacgc ttgatggtca
     6001 agcccaacca tttgggcgcc ggttcctcat caagcgacag gtgggccgga cgcgtgcgcg
     6061 gatgccaggt cgtcagcagg tagtctgcgc gcttgccgac gaaggcgctg tagcgcgaac
     6121 gcatcagcgt ctccgggtca ggcgccgctt cgccggcgtg gaagcggccg cagcaggagg
     6181 ccagcggccg attgctgccg caggggcagg tcatggtttc catttcttcg agcaggaagc
     6241 ggcggccgag ggcgaagccg gccgccgctc gcccgggcct cagtgcactt ggcgagtgct
     6301 gctcggttga tcggggtccc ggaaaaccgg ttcgtcgatg tccatcgttg cggcgatcag
     6361 ggcttccggg atgcgggatt gccgtgagaa ccgctggtcc gcggcaaggc tcagcccgag
     6421 ctggagtgcg cgctgcggat gctcagccgc aggcgcgggc gcatccgagg cttcaggcct
     6481 gatgatctcg taagtctcat tgtcgaactc gattcggtcc agcgtccact tgcggttcat
     6541 ggcacacctc cttcggtctt gtccgcgcgg gtcgggtact gccgggaccc gcgtgctctg
     6601 tttcatgcat cagccgtgcc agccatcgct tgacgccagc gaacacggcg caagctactg
     6661 attattggtg atatgcgctt gggcggggtt tttctcccag gtcatcgtgg cgcgacgatt
     6721 ggggcaggct gcccgcattt ggtgccgtca tttgcaccac acatgccgta ttcgggaagt
     6781 cttcaggcgg tcacgggctt cctcgcgcag atggattggg tgaccgcctt gtggctggcg
     6841 tcggccgcgt cgatcgactc gaccccttcg cgaaggcctt cgatagccca gccggccttg
     6901 ttcgcgagtg ccgccagttc gccgggcgcg agcaggaatt cgggccggct tggtcggccg
     6961 tattgctcgt tgcccagcat gaaggtttcg tagatgagta ggccgccggg cgtgaccgtg
     7021 gcgaggatct gcggaaacag cgggcggtgc agataacggc aaacaacgac cgcgtcgaag
     7081 gtctcgccaa ccagtggcca cggggcgccc tcgaggtcgg cctgccgcgt ggaaatcccc
     7141 ggaacgccgg ccaagccggc aagtgcctcg gcgtcgcggt cgacggcgcg gaccacaaac
     7201 ccctgggccg ccagccagcg cgcgttacgg ccgctgccgc aggcgaggtc gaggacgcgg
     7261 gcgccggcgg gcaggctggc ggcccagcgc atgacccagg gcgaggcatc accgccgcgg
     7321 tgtgcccggt gcgcggtgtc gctcatgccg cggcgattcg aaccggtatg cggcggttgc
     7381 cgaagctgcc cgtgtagtgc gcgaagcggc cggcgaggcg ggtaccgcag tcggggcagg
     7441 tgccatcgtc ccgcaagcgg taatcgcgta tcgcatagcc gtcgcgaacg atcagcgcgc
     7501 gcgcgcagcc ggtgcaatgc gtggtgccgc cggcgagatc gcggacgttg ccggtgtaga
     7561 catgacggag gccggcatcg agtgcctgtc gccgggcgcg cgtaagcgtt gcggccggag
     7621 tcgcgggacg gtcggtgagt ttccagtcgg gatggaaggc ggtgaaatgc agcggtacct
     7681 cgggaccgag ctcgcgggcg acccaggctg acagttctgc gatctcggtt tcgctgtcgt
     7741 tgagaccggg aatcagcaac gtggtgattt ccagccagca gttggtttcg tgccaaatcc
     7801 aggccagcgt atcgagcacc ggctgcaggc gcgcaccaca ttgccggacg tagaactcgt
     7861 cggtgaaggc cttgaggtcg acgttggcgg cgtccatgtg gtggaagaag ggctcgcgcg
     7921 gcagcgcgtg gatgtagccg gcggtgacgg cgacggtgtc gatgccgatg gcgtggcagg
     7981 cctcggcgac gtcgatcgcg tattcgtaaa agatcaccgg gtcgttgtag gtgaaggcaa
     8041 ccgagcgggc gccggcggcg tgtgcggcgc gggcaatcgc ctcagggctc gcttggtcca
     8101 tcagtcgatc catgtcgcgc gacttggaga tgtcccagtt ctggcagaac ttgcaggcga
     8161 ggttacagcc ggcggtgccg aaggaaagga tgctgctgcc cgggtagaag tgattgagcg
     8221 gcttcttctc gaccgggtcg atgcagaatc ccgacgagcg gccgtcggtg gtgagcacca
     8281 tctgctgacc atcgtaggcg cggacgaagc aggcgccgcg ctggccctcg tgcagccggc
     8341 agtcgcgcgg gcagaggtcg cattgaacgc gaccatcgtc gaggcgatgc caccactggg
     8401 cggccgtatt gccgctgtct ggcaggaccc gcgcttcact catgccgggc catttcgtcg
     8461 tcgtcttcgc accatttgcg caccccatag cgcgacaggc ggacatctgg atgccagaaa
     8521 tcgggcgggt agccggcctt ctccttgagg cgggcgagaa agtccgccgg cgccggcagc
     8581 gactcccaga cctgcggcag gaaggtcgcg cggcgccccc cgtagtcgaa gatcaggccg
     8641 tcggtgcccg gccgcagcgc ggcgatcgct gcggcttcgc tggcgacgac gaagggctcg
     8701 ggtggcgcga gcagcgagac ctcgacccgt gtgccgggcc actcctcacg acggagcggg
     8761 ggaaatcttg ggtcgcgaaa cgccgccgcc agcgcgtttt cggccacgtc ggtaatcagc
     8821 ggccgccgtg cttcgagcgt gccgatgcag ccgcgcaacg cgccggcgcg ggtcagcgtg
     8881 acgaagctcg cgccttcgcc ggcgggcagc gcggcgcgcg cggcggcgag ttcatctggg
     8941 tcggtgtttg cgccgaggcg ttcggcgatg gcatggcgcg caactgcgag cagcgcccgg
     9001 cccggatcgc gcgcggcttc agcaggcatg gtcatcgtcg tagaagccga tcgcggcgta
     9061 gccgacgacg cgctggcggt cgccggccgt gtcgcccgag ttgcagtgcg cgagcagccg
     9121 cgcctgccag ccgcgcgcag cagcgacgcg cagcaggccg ttgattggcc gggcgccgca
     9181 ggcctgctcg ccgagcaggc cgctttgtag cgcaaggatt cggtcgatcg tctcgcggtc
     9241 aatccgccgc gcttcgtcgt agcgatggaa gtgcgacagg tcggagctga tcagcagcag
     9301 cgtgtccggc tcgtcacaga aggcctcgat cagcggcccg acaagggccg gcggtgcctc
     9361 gccgactact accggcagca gcgagaattt gccgagcagc gtctgcagga atggcaattg
     9421 gacttcgagc gaatgttcct gcacgtgtgg ctggtcgctg gcgatgatgc cgggcagcgc
     9481 cagcgccttg tcgcgcgcgg ctgtgtcgat ggcgatcgcg ccgagcggcg acgcgaatcg
     9541 cgcgacctgg ggcacggcca tgccggcaaa ggcgacgcgg tgcgcagggc cgagcaggac
     9601 gacccggcgg atgccagcgg caaagggttc cagcgtgcgg taggcagtgg ccgcgatcgc
     9661 tcccgaatag acgtaaccgg cgtgcggaac gaccacggcg cgcggccgtg atggcggcag
     9721 cgcttccgga accggcgcgg cgcggtaggc atcggcgatg gccgcccgaa ggggcccctc
     9781 gtgggcggga tagaaggtgc cggcaacggc ggcaggccgg gtgccagctc ttggatcgat
     9841 ggtcatgttg ctgctcccga gagtacgaac cataccgcgc cggcgacgag gccggttgca
     9901 atgaggccaa atccggcagc gacccgacgg gtcagcaggg caccaccgac catgctgacg
     9961 atgccgagct tgaatacgag gttggacagc gtggcgatga gaatggccgt gacggcgtca
    10021 ctcgccgaga gctttccaag gccatagaga cgcaggctcg acagcgtgat ggcgtcgacg
    10081 tcggtcaggc cggagactac ggcaacgagg taaaggccct tgctgccggc gtagtccgag
    10141 agcgccgcgg agattaccaa gacaagtccg tacacggcac cgaaagtcag tgccgtgcgg
    10201 atttctgcgg ggttcgttag ttcgagcgcg ggcacgtcct cgcgcgagtt caggccgcgc
    10261 cagatcgcca cgacaaaacc ggccccaagc accagaccga tggccagcgg tggcaataga
    10321 atgggcagca gcgttggttg caggactgcg ccgtagatcg acaggcggac gagcacaacc
    10381 aagttggcga gcaggatcac cacggttgcc agtcgcaaga ggtgggtgcc gctggcagcg
    10441 tgtcgcgaaa ataccagtgt tgtggcggta ctcgaggcta gaccaccaag cacgccgaga
    10501 atcggggcgc cgtggtgctg gccgacgata cggagcgccg cgtagccagc caggctgacc
    10561 ccggagatca gtacgaccat ccaccagagc tgagccgggt taagcgcgtc gtagggtccc
    10621 atatcggaat tgggcaggat cggcagaatc accagcgaga gcacgccgaa ctgcaggatc
    10681 gagatcagat ctttgcgcgt caacttctgc gagaacaggc gcaactcggc cttgaagtag
    10741 agcagcatgg tggtgacgat cgacagcatc accgcataga tgcggaaacc gtgccagacc
    10801 atcgccccgt agacgaagca gagcagtacg gcgacgacgg tcgtcgtgcc ggggtcctgc
    10861 gggtcgggat gggtgacgta ggcggagatg gtcatgactc ccactagcaa caggccaacc
    10921 gccagcatcc agggcgagcc agtcttttcg cctagcagcg cggcgaccgt gccaaacagg
    10981 gcgactaggc cgaaggtgcg caagccggcg cgcgccgccg ggttgcgttc gcgctcgagc
    11041 cccatcaaca gtccgatgcc gaggcttagg gagaaaacct gcaggaactc cagaccgacc
    11101 tcggtcaaca acgcgtcgcg catcatttcg cctgttctca cccgggtcgc acgctcggtg
    11161 agccgggctt gtcgggtaaa attagcccat gccccgctac tgcgcaatcg tacccgccgc
    11221 cggcagcggc gcccgcttcg gcgccgagac ccccaaacag tacctttcgg tcggcaatca
    11281 tccgttgctc taccacgccc tgcgtccgct gctgcgccag ccgcagattg aacgcgtggt
    11341 cgttgtgctc tcgccagccg accgcttctg gcagcactac gaatgggatg atccggcggg
    11401 caagctgatg gtcatccgcg agggcggcgc cagtcgcgcc gacagcgtgc gcgccggtct
    11461 cgcggccgtg caggcgaacc tgcacgaatc cgactgggtc ctggtgcacg acgccgcacg
    11521 cgcctgcctt gcgtccgccg acctcgattc gctgatcaca gagctgacgg acgatccggt
    11581 aggtgggctg ctcgcagtgc cgctcgccga tacgctcaag cacggcgacg cggccgggcg
    11641 cgtcgaccgg acgccaccgc gcgacgggct gtggcaggcg cagacgccgc agatgttccg
    11701 cgccgggatt ctcggcgaag cgctgcgcgc ggccaaggcg gtcaccgacg aggcgtcggc
    11761 ggtcgaggca ctcggcctca agccacgcct ggtcgccgcg caatatccga atttcaaggt
    11821 tacctacgcc gccgatctcg atctggcgcg gctgattctc gaggcgcgtg agcgagtgga
    11881 agtcgtccat gcctgatcca atggcaacgc tgccgttccg ggtcggacag ggctacgacg
    11941 tgcacgcgct ggttccgggt cggccgctga tcgtcggcgg cgtgcgcatc gagtcgccgg
    12001 tcggtctgct cgggcattca gatgccgacg tgctactgca tgcgatcacc gacgcactgc
    12061 tcggtggcgc cgggctcggc gacatcggcc gacattttcc tgacaccgat gcgcagttcg
    12121 ccggtgccga cagccgcgtg ttgctgcgcg cgacgcgcgg gaagctggcc gaggccggtt
    12181 ggcgagtcgg caacctcgac gccaccgtag tcgcccaaaa gccacgactg gcggcctaca
    12241 ttcctgccat gtgcgtggcc attgccgaag acctcggtat tgccgtcggc gcggtcaacg
    12301 taaaagccaa gacccacgag cggctcggct tcgaggggcg aggcgagggc attgccgcgc
    12361 aggctgtcgc cctgttgctg ccccgttgac gcgccctggc accgatgcgg tggcggtcga
    12421 ccggcaccgt ctcttctttg ccgtgtggcc cgatcccgtg acggcggcga ccctggccga
    12481 cgaggcgcag cggctcgctc cccgactgga aggccgcgcg atgcgggccg agacgctgca
    12541 cctgacgctg gcctttctcg gcaatcgttc cggcgcacaa cgcgaccggc tgtgcgaagg
    12601 gctcgaccaa gccaatcgca gtggctggcc gggcggattc gcgcttaggc tcgaccgtct
    12661 gcagcagtgg cgacataatg gcattgtcct cgcggcgagt tccgagcgct gcgagcccct
    12721 gcaccgactg gccggattgg ttagcgagtt gtcgagaaca tgtggaatcc atgtgcccga
    12781 acgtcccttc gttccgcacc tgacgctgct gcggagctgt cgcggcgagc tgccggcgcg
    12841 caccggcggg gatggcccgt ggcattgggc ggtacgcgcg tttcggctgg tcgagagttt
    12901 tctcggtcca gccggcgcca gctaccgggt gatcggcgaa tgggccttgc cgtcggcccc
    12961 gcgggccgat ggaggcggat agcgtgaatc ggccgtttct tccgctggct ccgaccggtc
    13021 tcaagatttc cgggcaggaa gccaccgaaa tcgccagttt cgtactgatc gccaatgcac
    13081 cagcaggcga ccgggcagcg atcagcagca gtgtcgaggc gcacgccggg cggcagtttt
    13141 tcctgcgcta taccgatgag attgctggtc tgcgcgaggc gcttgagtgc tgctcctggg
    13201 atgcggttgt ccttgattgc cgaggcggca gcgtggattt cgatgcctat ctgcatgtca
    13261 tacgccatta ctggccgaac gcgaacatcg ttgtctgggg cggcgagcaa gcgggacagc
    13321 cggtcgagga tcggcggttg ctgcgtgtga cggagcgggc cccggcggcg ctggtggcct
    13381 cgctggccga ccttccgcgg acttcgccgg aatcggcaac gccagcacgg cgcagccgca
    13441 aggaagcgga cgcagcggcg cacctgcagc aactcgccga caacattccc gagtgcctgt
    13501 ggcttcagga cctcgtcgag cgcgaagtcg tctatgtgaa tccggcatac gcatcgatca
    13561 ccggtttcga gccggcgatg ccggtcaatg actggcgggc gctattcgac tgcgtctacg
    13621 tcgacgatgt ggcgcgcttc gccgaggcgc aggcgagggc accctttggc gggctcgatg
    13681 aggagttccg aattcgccgc gccgacggcg aactgcgttg ggtgcacctg cgcacctttg
    13741 cgatccgcga tcgcgagggc ctgatcgccg gcgtcggcgg agccatgcgc gacatcagcg
    13801 cggcggtcgc ccagcagaag catctctacc aagtgacgca cttcgacggc ctgacggcac
    13861 tgcccaaccg gatgctgttg caggagcggc tgactgcctc ggtggcgatg gcccggcgca
    13921 acgactggct gctcggcctg ctgctaatcg acatcgaccg attcaagatc atcaacgaca
    13981 cgctgggcca tgcggtcggc gacgaactgc tgcagcaggc ggcgatgcgc ctgcgcgcct
    14041 gcctgcggga cagcgacacg gtcggtcgta tgggcggcga cgagtttgcc gtggtgttgc
    14101 ccgacatgga tagcgccgaa atggcagcga tcgtcgcgcg caaggtggtc gaggtgatcg
    14161 ccgagccctt tgtgatcgac gggcaggagt tgttcgtttc ggccagcgtg ggcattagcc
    14221 tttatcccga cgacgcagcc gacatcgacg tgctggttcg caatgccgac gcggcgatgt
    14281 atcgcgccaa ggattccggg cgcaacgcct acgccttcta caccgccgaa atgaacgtcc
    14341 gggcgcggcg ccggctgggt ctggaaaccg acctgcggca ggcgctggcg cgcgacgaat
    14401 tcgagctgca ctaccagccc aaggtcaatt gccgcagcgg cgcgatcgcc ggggtcgagg
    14461 cgctgatccg ctggcgacac ccggagcgcg gcctggtcgg gcccgacgaa ttcatccctc
    14521 tgctcgagga aaccgggctg atcgtgccgg tcggcgactg ggtgatgcgc accgcctgcg
    14581 cgcagatgca ggaatggcac accgccggat ttgctcaact cgacatcgcc gtcaacgtct
    14641 cggcgcggca gatggaaggg cgccggctga tcgaagccgt gcgggtggct ctagatgcga
    14701 ccggcctgcc ggcggcggcg ctcgaaatcg agctgaccga aagcctgctg atgcgcgatc
    14761 cgccggccgt ggtcgacatg ctctccgaac tgaaggcgat ggggctgcag gtcgccgtcg
    14821 acgatttcgg tactggctat tcgagccttt cctacctcaa gcgttttccg ctcgacaacc
    14881 tcaaggtcga ccgcgccttc gttcaggaca tcgcggcgag cgccggcgat gcctcgatca
    14941 cgcgcgcggt gatccacatg ggtcacgagc tggcgctcaa ggttgtcgcc gagggcgtcg
    15001 aaactgccga acagctcgaa gcgctacaga tcgccggttg cgacgagatt cagggttact
    15061 acttcagccg cccgctgccg cccaaggaca ttctcgaatt gctccgcagc ggtcgtacct
    15121 tgccggttcg cagtggtgcc gacggtgaat acgagcaacg tagcggcctt gatccggcga
    15181 tcgcgcgggc gctgtccgac ctgcgtcagg aacgcgaccg acaggccgcc gccgccgatg
    15241 ccgcgcggct gctctggcgg cagacgctcg aagcctttgc cgagcccgtg gtcggggtcg
    15301 accgggcagg gcaggtggtc ttcgccaacg atgcggccga cgaggctttc gccgaatgcg
    15361 gcttgcatcc gggcgtggtg attgccgact gtcttccgcc gctggcattg gcgcttggcg
    15421 gtgacgcgag cgttgcggtc acgggcgccg atggcacgcg ctaccgggtg cggatctcgc
    15481 gcctgcaggc caggcagcca tcggccgctg gcgatcgcag tggcgcgacg ttgttgtggt
    15541 tcagccgcga cggggaggtt tcgtgatcga tttcggttcc cgccagtcgg gcgaacttct
    15601 cgtgccggtc gctgccggcg agtggcggcg ccgccgtcgc gaccagccga tgcgctgcga
    15661 tccgctggcg ctggctaggc gacgccaatc cagccgcgcc ctgcggcagg ccctcggcgg
    15721 catcagcgct gccaccctga ttcgttgcgc catgtcggca ggcataactg gagaacaaca
    15781 agaatggtga agctcagcgc cgaatccgta gcgaattcgc tgcatcagct gccggcgctg
    15841 cacccggtgg tggtcaagtt gctcgaatcc ttcgagaagg ccggcgtcga cacgcagtcg
    15901 ttggtccacg aaattgccca ggatcaggcg ctggtaacac gcgtgttgcg tctggccaat
    15961 tcgtcgttct acgggctgcc gagtcacgtc gattcgctca aggacgcggt gatgattctc
    16021 ggctttcgca ccgtgcgcgc ggtcgcggtc gcggtgtcat tgaccgattg tttctcgagc
    16081 cgcaaggtgc cgggattcga taccaacgcc ttctggcaac atagcgcggc ggtcggcatg
    16141 gctgcccgcg agattgccgc aatcgttaag cggcccaccg atgtcgcctt caccgccgga
    16201 atcgttcacg atatcggcgt cattgcgctg ctttggctcc atccgaagga tatggcagcg
    16261 gtcttggcgg cctgtcgcga caacggactg ccgctagacg tcgccgagcg cgaggttttc
    16321 ggcgtcgatc actgcgcaat cggcgcagcg ctggccaggc actggaatct gccggaggcg
    16381 ctggtcgaat cgatcgccag ccacgagcag ccggccaacg ctgaagcggg ctcgctggcc
    16441 gacatcgttc acgtcgccaa cgcgatcatt cacgactacg gcctgcctgc gattgaggtg
    16501 attaccgtct cggcggtatc tgacctggcg atgaaccggc tgggcatc
//
LOCUS       DATDAA010000125         3727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_323551,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000125 DATDAA010000000
VERSION     DATDAA010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323551"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1668)
                     /locus_tag="VIS73_04025"
     CDS             complement(<1..1668)
                     /locus_tag="VIS73_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEY5762355.1"
                     /translation="MRKAGFWKSDWFLGVAVSLVLLMLAQTPAVQSLERAAYDIGVRM
                     SDRQPSDRIAVIAIDEVSIANLGRWPWSRELHARMTELLAQAQAKVIVNTVFFTEPQR
                     DPGYDYVLRLIDLYVRGAAPAGEGLAAPTLPPSISAMGAVLQEAESTLNTDRQLAAAI
                     QSAARVQLPMLFELGESLGRPDPLPEAIQRNRLVNVGGDGFAWPAVQALPPIPEFSAG
                     AAGIGHLNSVPDVDGGTRSEALVVRYFDQYYPALSLLAAAKSLNLDAGDIQVVLGEEV
                     RVGNLRIPTDGATQMLTHFYADRDGRSAFVADSFYDVLSGRIPASKYRDKIVLIGSTA
                     AGLGNAQVTPVSPVMAPVTTMAHTVSSILREHFFVNPLWAGFVTHGSALLVALYLIIL
                     LPRLSAALAAATTGGLLVLLLGAHFGLMVGASLWIPLMSTAMLLTVGHLLLTTKRFLV
                     TERGKEQSEAVSAESNRMLALAFQGQGQLDMAWDKFRQVPMSDSLLENLYNLALDFER
                     KRQFNKAEAVFAHMAAYRLDFKDLAQRMARAKQLSETVMLGGGGSHPG"
     gene            complement(1858..2751)
                     /gene="sucD"
                     /locus_tag="VIS73_04030"
     CDS             complement(1858..2751)
                     /gene="sucD"
                     /locus_tag="VIS73_04030"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY5762356.1"
                     /translation="MSILINKDTKVITQGITGKTGQFHTEKCQEYANGKNCFVAGVNP
                     KKAGEKIFDIPIFGSVKDAKQATGATVSVIYVPPAGAAAAIWEACEADLDLAICITEG
                     IPVRDMLEVRNKMKQKEAKGGKKTLLLGPNCPGLITPDEIKIGIMPGHIHRKGRIGVV
                     SRSGTLTYEAVAQLTEIGLGQSSAVGIGGDPINGLKHIDVMKAFNDDPDTDAVIMIGE
                     IGGPDEAEAAEWCKANMKKPIVGFIAGVTAPAGKRMGHAGALISGGADTADAKLAIME
                     ACGFKVTRNPSEMAKLLKAMI"
     gene            complement(2764..>3727)
                     /gene="sucC"
                     /locus_tag="VIS73_04035"
     CDS             complement(2764..>3727)
                     /gene="sucC"
                     /locus_tag="VIS73_04035"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY5762357.1"
                     /translation="DEVKAHASAILGMQLKTHQTGPEGQKVRRLLVEDGADIKKEYYV
                     ACLTDRATQKVAMMASSEGGMDIEEVAHKTPEKIIKVFVDPLVGITDAQCDQLAKGIG
                     VPEASQAKARDVLKKLYTCYMETDASLAEINPLILEGDGNIKALDAKFNFDSNALYRQ
                     PEIVAYRDLDEEDADEIEASKFDLAYISLDGNIGCLVNGAGLAMATMDTIKLFGAEPA
                     NFLDVGGGATTEKVTEAFKIMLKNPKVKGILVNIFGGIMRCDTIATGVVTAAREVHLS
                     VPLVVRMKGTNEDIGKKILKDSGLPIISADTMAEAAEKIVAAVK"
BASE COUNT          653 a         1171 c         1189 g          714 t
ORIGIN      
        1 acccggatgc gagccaccgc cgccgagcat gacggtttcc gacagctgct tggcgcgcgc
       61 catccgttgc gccagatcct tgaagtcgag gcggtacgcg gccatgtgcg cgaacacggc
      121 ctcggccttg ttgaattgcc gtttgcgctc gaagtccagc gccaggttat agaggttctc
      181 caacagactg tcgctcatcg gcacctggcg gaacttgtcc caggccatgt caagttgccc
      241 ctgcccctgg aaggccagcg ccagcatgcg attcgattcg gccgagaccg cctccgactg
      301 ttccttgccg cgttcggtga cgaggaagcg cttggtggtc agcagcaggt ggccgaccgt
      361 cagcagcatc gccgtactca tcagtggaat ccagagcgag gcgcccacca tcaggccgaa
      421 gtgcgcgccg aggagaagca cgagcaggcc accggtggtt gcggcagcca gggcagccga
      481 caggcgaggc agaagaatga tcaggtagag cgcgaccagc agcgcgctgc catgcgtgac
      541 gaatccagcc cagagcggat tgacgaagaa atgttcgcgc aggatgctcg acaccgtgtg
      601 cgccatcgtc gtcaccggcg ccatcaccgg cgacaccggg gtaacctgcg cgttgccaag
      661 gccagccgcg gtcgacccga tgaggacgat cttgtcgcgg tacttggacg ccggaatccg
      721 ccctgacagc acgtcgtaga acgaatcggc aacaaaggcg gaacgaccgt cacggtcggc
      781 gtagaagtgg gtgagcatct gcgtcgcacc gtcggtcggg atgcgcagat tgccgacccg
      841 cacctcctcg ccgaggacga cctggatgtc gccggcgtcg aggttgaggc tcttcgcggc
      901 ggcgagcagc gacagcgccg gatagtactg atcgaagtaa cgcacgacca gcgcttcgga
      961 gcgcgtgccg ccatcgacgt cgggcacgct gttcaggtgc ccgattccgg ccgcgccggc
     1021 gctgaattcg gggatcggcg gcaatgcctg tacggccggc caggcgaatc catcaccgcc
     1081 gacgttgacc aggcggttgc gctgaatggc ttcgggcagc gggtcgggac gacccagcga
     1141 ctcgccgagt tcgaacagca tcggcaactg cacgcgcgcc gccgactgaa tcgcggcggc
     1201 cagctggcga tcggtgttaa gcgtggactc cgcctcctgc agcaccgccc ccatcgccga
     1261 aatcgacggc ggcagtgtcg gtgccgccag cccttctccg gccggcgccg caccacgcac
     1321 gtaaaggtca atcaggcgga gaacatagtc gtaaccgggg tcgcgctgcg gctcggtgaa
     1381 aaaaaccgtg ttgacgatga ccttggcctg agcctgcgcc agcagctcgg tcatccttgc
     1441 atggagctcg cgtgaccacg gccagcgccc caggttggcg atcgacacct cgtcgatggc
     1501 aatgacggcg atgcggtccg acggttggcg gtcggacata cgcaccccga tgtcgtaggc
     1561 cgcgcgctcg aggctctgga ctgccggcgt ctgtgccaac atgagcagta ccagcgacac
     1621 ggcgacaccg agaaaccagt cggacttcca gaatccagcc ttccgcatac acgtcctcta
     1681 gacaggagtg ggcaagtcgc gctcgggtga tgccaagagt tgcattctta ccgtggccag
     1741 atgccgccgt caatgaagta tccggcggca caaggcaaaa cggcggatcg tcacgatccg
     1801 ccgttttggg tgccagcagg ccgcttccgg cgcgccggcc ttagtgcgtt accgcgttca
     1861 gatcatcgcc ttcagcagct tggccatctc cgacgggttg cgcgtcacct tgaagccgca
     1921 ggcttccatg atggcgagct tggcatcagc ggtgtcggcg ccgcccgaga tcagcgcacc
     1981 ggcgtggccc atgcgcttgc cggccggtgc cgtgacgccg gcgatgaagc cgacgatcgg
     2041 cttcttcatg ttggccttgc accattcggc cgcctcagct tcgtcgggac cgccaatctc
     2101 gccaatcatg atcactgcgt cggtgtcggg atcgtcgttg aaggccttca tcacgtcgat
     2161 gtgcttgagg ccattgatcg gatcgccacc gatgccgacc gccgaactct ggccgaggcc
     2221 gatttcggtc agctgagcaa ccgcttcata ggtcagcgtg cccgaacgcg agacgacgcc
     2281 gatgcgaccc ttgcggtgaa tgtggccggg catgatgccg atcttgatct cgtcgggcgt
     2341 gatcaggccg gggcagttgg ggccgagcag cagcgtcttc ttgccgccct tggcttcctt
     2401 ctgcttcatt ttgttgcgaa cttccagcat atcgcgcacc ggaatgcctt cggtgatgca
     2461 gatggcgaga tcgaggtcgg cctcgcaggc ctcccagatc gccgccgccg caccggccgg
     2521 cggcacgtag atcaccgaga cggtggcgcc ggtcgcctgc ttggcgtcct tgaccgaacc
     2581 aaagatcgga atgtcgaaga ttttctcgcc ggccttcttg gggttgacgc cggcgacgaa
     2641 gcagttcttg ccgttggcgt actcctggca cttttccgtg tggaactggc cggtcttgcc
     2701 ggtaatgccc tgggtgatga ctttggtgtc tttattgatc aggatggaca tgtcttgtat
     2761 tcctcacttg acggcagcga cgattttttc ggccgcttcg gccatcgtgt cggcagagat
     2821 gatgggcaga ccggagtcct tgagaatctt cttgccgatg tcctcgttgg tgcccttcat
     2881 gcggaccaca agcggaaccg acaggtgcac ctcgcgcgca gcggtgacca cgccagtggc
     2941 gatggtgtcg cagcgcatga tgccgccgaa gatgttgacc agaatgccct tgaccttggg
     3001 attcttgagc atgatcttga aggcttcagt gaccttctcg gtcgtcgcac caccaccgac
     3061 gtcgaggaag ttggccggct cggcgccgaa cagcttgatc gtgtccatgg tcgccatcgc
     3121 cagaccggcg ccattgacca ggcagccgat gttgccatcg agagagatgt aggcgagatc
     3181 gaacttcgat gcctcgatct cgtcagcgtc ttcctcgtcg aggtcgcggt aggcgacgat
     3241 ctcgggctga cggtagagcg cgttcgaatc gaagttaaac ttggcgtcga gcgccttgat
     3301 gttgccatcg ccctcgagga tcagcgggtt gatctcggcc agcgaagcgt cggtttccat
     3361 gtaacaggtg tagagcttct tcagcacgtc gcgggccttc gcctgcgacg cctccggaac
     3421 accgatgccc ttggccaact ggtcgcactg cgcgtcggta atgccgacga gcggatcgac
     3481 gaagaccttg atgatcttct cgggggtctt gtgcgcgact tcctcgatgt ccatgccgcc
     3541 ttcgctggag gccatcatcg cgactttctg cgtcgcacgg tcggtcaggc aggcgacgta
     3601 gtactccttc ttgatgtcgg caccgtcttc gaccagcagg cgacggacct tctgtccctc
     3661 aggacccgtc tggtgcgtct tgagttgcat gccgaggatc gccgaggcgt gcgccttgac
     3721 ttcatcc
//
LOCUS       DATDAA010000126         3360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_148580,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000126 DATDAA010000000
VERSION     DATDAA010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148580"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..768)
                     /gene="msrA"
                     /locus_tag="VIS73_04040"
     CDS             complement(187..768)
                     /gene="msrA"
                     /locus_tag="VIS73_04040"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY5762358.1"
                     /translation="MTQQDFKDPAGTEVAVLGGGCFWCIEAVFQRLRGVHSVVSGYSG
                     GSRPAPSYEQVCTGASGHAEVVRIAFDPQVIDFASLLDVFFAVHDPTTLDRQGHDIGS
                     QYRSVIFCQNAGQRAEAAAAIRRIDAAGYWGAPAVTELAGKEPFWPAEDYHRNYYDGH
                     RSQPYCQAVIAPKVAKLQVAFKDLLGPPRGPAR"
     gene            complement(883..1797)
                     /locus_tag="VIS73_04045"
     CDS             complement(883..1797)
                     /locus_tag="VIS73_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5762359.1"
                     /translation="MDRLLAMKVFLAVVDSGAFARAAQQLNLSTTAVSRHVAQLEDLL
                     GASLLQRTTRRLSLTETGAAYYERCRQILADVEEAEAQAASAEAQPRGTLRISLPYSF
                     GLRYVSPLLPKFRAQYPELALDVSFADRMVDLVEEGVDMALRITRAPNTTLVARQLAP
                     VRIVCCASPAYLARHGTPKTPDQLREHACLIYSYVGDNWEFTSADGAKIGVQVRGPLR
                     ANAGDVLNDAALAGEGIVLQPTFLVADDLRQGRLIRLFPAYALESSVAYAVYLPGLRR
                     TARVQAMVRFLQEAFAGDDPPWDQNLPT"
     gene            1906..2472
                     /locus_tag="VIS73_04050"
     CDS             1906..2472
                     /locus_tag="VIS73_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY5762360.1"
                     /translation="MTVQPYPLSRRSLHWLTVLLLVAAAAVGLYMTGLKLTPTKLQLY
                     SYHKWIGITVFLIALARLWLAYRLPAAEHPAHAAWQQFAARQTHRAIYVLLVLIPISG
                     WLMSSAKGFQTVYLGVLPLPDLLAKDRELGDLLASVHQALNLTLAGLVAAHVGAAIWH
                     HFILRDGLLRRMWPAHAEKSNAIPRSPA"
     gene            2469..3023
                     /locus_tag="VIS73_04055"
     CDS             2469..3023
                     /locus_tag="VIS73_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY5762361.1"
                     /translation="MKKSILIAALLLAASLPVQAVTYTKVDPGKSTMIFVSKQMGVAV
                     DGRFKRFSTTLRFDPSTPEKSQAELAIDLASVDTGVPEADEEVVGKDWFDVARYPQAT
                     FVSRSVKALGGGRFEAVGELTVKGTTHPVTAPFTVREAGGRAIIEGSLTILRGDYAVG
                     SGAWADYGTVANEVILKFRVEAMP"
     gene            3072..>3360
                     /locus_tag="VIS73_04060"
     CDS             3072..>3360
                     /locus_tag="VIS73_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY5762362.1"
                     /translation="MTLAKTLKKSALALTLASVAGLAAAASESYVIEPTHTLPKFSYN
                     HFGYSTQSSVFTKTSGKITLDRTAKKGSVNVTIDAKSVETGVPLFNEHIQAA"
BASE COUNT          612 a         1147 c         1039 g          562 t
ORIGIN      
        1 acgccgaaac cgcgcactac gtcgcctcga aaatacccga cgcgatcctc aacatctacg
       61 aaggcacgga ccactcgccg caccagtggc agcgcgaacg ctttgcgcgc gaactcgtgg
      121 atttcatcaa cacctaggtc cgcggggcgg gaaagttcaa cgggtggttc cgcgtggcgg
      181 tattgcttaa cgagcgggcc cgcgtggcgg gcccaggagg tccttgaagg caacttgcag
      241 tttcgccacc ttgggcgcga ttaccgcctg acaatagggc tggctgcgat gaccgtcgta
      301 gtagttgcgg tggtagtcct cggccggcca gaagggctcc ttgccggcca gttcggtcac
      361 cgccggcgca ccccagtaac cggcggcgtc gatgcggcgg attgccgctg ccgcttcggc
      421 acgctgtccg gcgttctggc agaagatcac tgagcggtat tggctgccga tgtcgtggcc
      481 ctgacggtcc agcgtcgtcg ggtcgtgcac ggcaaaaaag acatcgagca ggctcgcgaa
      541 atcgatcact tgcggatcga aggcaatgcg caccacctcg gcgtgcccgc ttgcaccggt
      601 acacacctgc tcgtagctcg gcgccggccg ggaaccgccg ctgtaaccgg acaccaccga
      661 gtgcactccg cgcagccgct ggaaaaccgc ctcgatacac cagaagcaac cgccgccgag
      721 aacagccacc tcagtgccgg ctgggtcctt gaagtcctgc tgggtcatgg ggtgcctatc
      781 atcaatggcc agaggcctgt cggaccgccg acggcgcggc gagttcggcg tgcatcacga
      841 atgcccggcc gcgcgatcga gcccgtcgcg gcgcgaccgc gctcacgtcg gcagattctg
      901 gtcccagggc ggatcgtcgc cggcaaaggc ctcctgcagg aagcgcacca tcgcctgtac
      961 tcgcgcagtg cggcgcaggc caggcaaata gacagcgtag gcgaccgacg attcgagtgc
     1021 gtaagctgga aacaggcgaa tcagccggcc ctgccgaagg tcgtcggcaa cgaggaaggt
     1081 cggctgcaag acgatgcctt cgccggccag cgccgcgtcg ttgagcacgt ccccggcgtt
     1141 ggcgcgcagc gggccgcgca cctgcacgcc gattttcgcg ccgtcggcag acgtgaactc
     1201 ccagttatcg ccgacgtagc tatagatcag gcaggcgtgc tcgcgcaatt gatcgggcgt
     1261 cttcggcgtg ccgtggcgtg ccaggtaggc cggcgaggcg cagcagacaa tgcgtaccgg
     1321 cgccagctgt cgcgcgacca gcgtcgtatt cggcgcccgg gtgatgcgca gcgccatgtc
     1381 gacaccctcc tcgaccaggt cgaccatacg gtcagcgaag ctgacgtcga gcgccagttc
     1441 ggggtattgc gcgcgaaact tcggcaggag cggcgagacg taacgcaggc cgaagctgta
     1501 gggcaggctg atgcgcagcg tgccgcgcgg ctgtgcctct gcgctggccg cctgcgcttc
     1561 tgcctcctcg acgtcggcga gaatctgccg gcagcgctca tagtacgcgg cgccggtttc
     1621 ggtcaggctg agccggcggg tagtccgctg cagcagcgac gctcctagga gatcctcgag
     1681 ttgcgcgacg tgcctcgaca cggcggttgt cgacagattg agctgctgcg cggcgcgagc
     1741 gaaggctccg ctgtccacaa ccgccagaaa gaccttcatc gccagcagtc tatccatggt
     1801 tcccaattat tattgctaat tgcgggatta taaattgtct atccaaggct ttatgaatga
     1861 aaatcgggat gccagactgc atggcatcgg aatcgaaacc cagccatgac cgtccagccc
     1921 tatcccctgt cgcgccgcag cctgcactgg cttaccgtgc tgctgcttgt cgccgccgcc
     1981 gcagtcgggc tttacatgac tgggttgaag ctgacgccga ccaagctgca gctgtactcg
     2041 taccacaagt ggattggcat caccgttttc ctgatcgccc tcgcccggct ctggctagcc
     2101 taccgcctgc cggccgccga acacccggcg cacgccgcct ggcagcagtt cgctgcccgc
     2161 cagacccacc gcgcgatcta cgtactgctg gtcctgattc cgatctcggg ttggctgatg
     2221 agttcggcca agggcttcca gactgtctat ctgggcgttc tgccgctgcc cgatctgctc
     2281 gccaaggacc gcgaactcgg tgatctgctg gcaagcgtcc atcaggcgct caacctgacg
     2341 ctggccggac tggtcgccgc ccacgtcggt gcggcgatct ggcaccactt catcctgcgc
     2401 gacgggctgc tacggcgcat gtggccggcg cacgccgaga aatccaacgc cattccccgg
     2461 agccccgcat gaagaaaagc atcctgatcg cggccctgct gctcgccgcc tcgctgccgg
     2521 tgcaggccgt cacctacacc aaggtcgatc ccgggaagag cacgatgatc ttcgtctcta
     2581 agcagatggg cgttgcagtc gatggccgct tcaagcgctt ttcgacgacg ctgcgcttcg
     2641 acccgtcaac cccggaaaag agtcaggccg aactggcgat cgacctcgcc agcgtcgata
     2701 ccggcgttcc cgaagccgac gaggaagtcg tcggcaagga ctggttcgac gtcgctcgtt
     2761 acccgcaggc gacctttgtc tcgcgcagcg tcaaggcgct cggcggtggc cgcttcgagg
     2821 ccgtcggcga gctgacggtc aagggcacca cgcacccggt gacggcaccg ttcacggtcc
     2881 gcgaggccgg tggccgagcc atcatcgaag gcagcctgac catcctgcgt ggcgactacg
     2941 ccgtcggctc cggcgcctgg gccgactacg gcacggtcgc caacgaagtg atcctcaagt
     3001 tccgcgtcga ggccatgccc tgagcccgac aattccaccc cgcaatcgcc caacctgaaa
     3061 cggagaaacc catgacactc gccaagaccc tcaaaaaatc cgccctcgcg ctcacgctcg
     3121 cctcggtcgc cggcctcgcc gcagccgcca gcgagagcta tgtgatcgaa ccgacccaca
     3181 cgctgcccaa gttctcgtac aaccacttcg gctactcgac tcagagcagc gtcttcacca
     3241 agaccagcgg caagatcacg cttgaccgca cggccaagaa gggctcggtc aacgtcacca
     3301 tcgacgccaa gtccgtcgag accggcgtac cgctcttcaa cgagcacatc caggccgccg
//
LOCUS       DATDAA010000127         4310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_218893,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000127 DATDAA010000000
VERSION     DATDAA010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218893"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /gene="dxs"
                     /locus_tag="VIS73_04065"
     CDS             complement(<1..1652)
                     /gene="dxs"
                     /locus_tag="VIS73_04065"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY5762363.1"
                     /translation="MQTTPLLDTVASPADLRKLDRDDLRQLCDELREYLIDTVSRTGG
                     HLSSNLGTVELTVALHYVYQTPDDRLVWDVGHQTYAHKVLTGRREMMPTLRQHDGLAG
                     FPKRSESDYDTFGVGHSSTSISAALGMAIAARQRGEERRAVAIIGDGAMTAGLAFEAL
                     NNAGVADADLLVILNDNDMSISPPVGALNKYLARLLSGGFYSGVRHATERVLGVAPPL
                     LEFAKRAEEHVKGMLTPGTLFEEFGFNYIGPIDGHDLDALIPTLENLRDRRGPQFLHV
                     VTRKGQGYKLAEADPVLYHGVSKFKPEIGIVGAPAGKAGAARPSYTQVFGDWLCDMAA
                     ADERLIGITPAMREGSGLVRFAEEYPDRYFDVGIAEQHALTFAAGLACDGAKPVVAIY
                     STFLQRAYDQLIHDIALQNLDVTFAIDRGGLVGADGPTHHGAFDLSFLSCIPNLVVMA
                     PADEAECRQMLTTAYRHDGPAAVRYPRGTGPGIAVDQALKEIQIGRGEIRRHGNKVAL
                     LAFGSMLAPAIEAAEQIDATVANMRFVKPLDRDLLRKLAEEH"
     gene            complement(1655..2545)
                     /locus_tag="VIS73_04070"
     CDS             complement(1655..2545)
                     /locus_tag="VIS73_04070"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEY5762364.1"
                     /translation="MSQEFDSWAARVQARVENALDARLPSTAKEPRRLHAAMRHSALG
                     GGKRVRPLLVHAAGLLTEANDAALESCACAVEAIHVYSLIHDDLPCMDNDTLRRGRPT
                     CHIAFDEATALLAGDALQALAFGWLAESPCADAQRANMVRLLAQAAGPAGMAGGQALD
                     LAAVGQSINLPDLEYMHALKTGALIRAAILLGAAAGPAPLPVESHEELDQFAKRVGLL
                     FQVVDDILDCTASTATLGKTAGKDMAADKPTYVSLLGLSAAKDLAAEIHADANAALRT
                     IGDRSARLQQLCDFIANRPF"
     gene            complement(2542..2802)
                     /gene="xseB"
                     /locus_tag="VIS73_04075"
     CDS             complement(2542..2802)
                     /gene="xseB"
                     /locus_tag="VIS73_04075"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY5762365.1"
                     /translation="MKNKRIDDADQVSADLPFEAALTELDAVLERMESGRLTLEESLT
                     TYRRGAALLRHCQSQLDDADARLRILEGESLKPLDIADGSSA"
     gene            2936..4042
                     /locus_tag="VIS73_04080"
     CDS             2936..4042
                     /locus_tag="VIS73_04080"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY5762366.1"
                     /translation="MSDIGNSAQLSPATTQLPVEWYFDERVFALEMKHLFADGPGYLG
                     HELMVPEIHDYRTLEWQDHGRMLVRQPDSVYAMSNVCRHRQAIMLQGAGNARNIVCPI
                     HRWTYGMDGALMGAPHFPANPCLNLDKQKLESWQGLLFGGPRSANRDLAGMQALTDFD
                     FSGYQLDRVEQHTCHYNWKTFIEVYLEDYHVAPFHPGLGNFVTCDDLSWQFGDWYSVQ
                     RVGITSLVKSGSAAYGRWQQAVRDYSLASGRAPRQGAVWLTYFPNVMVEWYPHVLVVS
                     SLIPQAVDRTLNVVEFYYPEEIAAFEREFIEAEQAAYMETALEDDDIGERMDRGRRAL
                     MRRGLGQAGPYQSPMEDGMQHFHEFYRRIIEPRL"
     gene            4124..>4310
                     /locus_tag="VIS73_04085"
     CDS             4124..>4310
                     /locus_tag="VIS73_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY5762367.1"
                     /translation="MLAASFGFACMGVCVKLAAARYAIAEIVFWRSVIAVSVSAVFMH
                     GYGLAFSTPQLRWQIGRG"
BASE COUNT          719 a         1324 c         1459 g          808 t
ORIGIN      
        1 caatgttcct cggccagttt gcgcagcaaa tcgcggtcga ggggtttgac gaagcgcatg
       61 ttggcaaccg tcgcgtcaat ctgctcggcg gcttcgattg ccggtgcgag catgctgccg
      121 aaggcaagca gcgcgacctt gttgccatgc cggcggattt cgccgcggcc aatttgaatt
      181 tccttgaggg cttggtcgac ggcaatgccg ggaccggttc ctcgtgggta ccgcacggcc
      241 gctgggccat cgtgccgata cgccgtcgtc agcatctgcc ggcactccgc ctcgtcggcc
      301 ggtgccatga cgacgaggtt gggaatgcag gaaagaaagg agaggtcgaa ggcgccgtgg
      361 tgggtcggac catcggcgcc gaccagcccg ccgcgatcga tggcgaaggt gacgtcgagg
      421 ttctgcagcg cgatgtcgtg tatcagctga tcgtaggcac gctggagaaa ggtcgaatag
      481 attgccacca ccggctttgc gccatcgcag gcgaggccgg cagcgaaggt gagcgcatgc
      541 tgctccgcga tgccgacatc gaagtagcga tcgggatact cctcggcgaa gcggaccagg
      601 cccgacccct cgcgcatagc cggggtaatg ccgatcaggc gctcgtcggc cgccgccatg
      661 tcgcacagcc agtcgccaaa gacctgtgta tagctgggtc gcgcggcgcc agccttgccg
      721 gcgggtgcgc cgacgatgcc gatctctggc ttgaacttcg agacgccgtg gtagagcacc
      781 gggtcggcct cggccagctt gtagccctgg cccttacggg tgacgacatg gaggaactga
      841 ggcccccttc ggtcgcgcag gttctccagc gtcggaataa gcgcgtcgag atcgtggccg
      901 tcgatcgggc cgatgtaatt gaagccaaac tcctcaaaca gcgtacccgg tgtcagcatg
      961 cccttgacgt gttcttcggc tcgcttggcg aactccagca gcggtggcgc aacaccgagg
     1021 acccgctcgg tcgcatgacg aactccgctg taaaagccgc cggaaagcag gcgggctagg
     1081 tacttgttaa gcgcgccgac cggcggcgag atcgacatgt cgttgtcgtt gaggatgacc
     1141 agcaggtcgg catcggcgac gccggcgttg ttcagcgcct cgaaggcaag gcccgcagtc
     1201 atcgcgccgt caccgatgat cgccaccgcc cgccgctcct cgccgcgttg gcgggccgcg
     1261 atcgccatgc ccagtgcggc cgagattgag gtgctcgagt ggccgacgcc gaaggtgtcg
     1321 tagtcgcttt cgctgcgttt cggaaagccg gccagcccgt cgtgctggcg cagcgtcggc
     1381 atcatttctc ggcggccggt gaggaccttg tgcgcgtagg tctggtgacc gacgtcccag
     1441 accagacggt catccggtgt ctggtacacg tagtgcagtg cgacggtcag ctcgacggtt
     1501 ccgaggttgg acgagaggtg gccgccggtg cgcgaaacgg tatcgatcag gtactcgcga
     1561 agttcgtcgc agagctgcct gaggtcgtcg cgatcgagct tgcgcagatc tgcggggctg
     1621 gcgaccgtat cgaggagagg agtagtctgc atgctcagaa aggccggttg gcgatgaagt
     1681 cgcagagttg ctgcaagcgt gccgaccgat caccgatggt ccttagcgcg gcgttggcat
     1741 cggcatggat ttcggcggcg agatccttgg cggccgacag tccgagcagg ctcacgtaag
     1801 taggcttgtc ggcggccatg tccttgccgg cggtcttccc cagcgttgcc gtgctggcgg
     1861 tgcagtcgag aatatcatcg acaacctgaa acagcagtcc gacgcgcttg gcgaactggt
     1921 cgagctcctc gtgcgattca acgggcagcg gcgccggccc ggcggcggcg ccgagcagga
     1981 tcgccgcacg gatcagcgcg ccggtcttca gcgcgtgcat gtattcgagg tcgggcaggt
     2041 tgatcgactg gccgaccgcg gcgaggtcga gcgcctgtcc gccggccatt cccgccggac
     2101 cggccgcctg cgcgagcagg cgaaccatgt tggcacgctg tgcgtcggcg cagggtgatt
     2161 cggccagcca gccgaaggcc agtgcctgca gcgcatcccc agcgagcagt gccgtcgctt
     2221 cgtcgaaggc tatgtggcag gtcgggcggc cgcgacgcag cgtgtcgttg tccatgcacg
     2281 gcaggtcatc gtgaatcagc gaatacacgt ggattgcctc gaccgcacag gcgcaggatt
     2341 ccagtgccgc gtcgtttgcc tcggtcagca gtccggcggc atggaccagc agcggcctta
     2401 cccgcttgcc gccgccaagt gccgagtggc gcattgctgc gtgcaagcgg cgtggttcct
     2461 tggccgtcga aggcaagcgc gcgtcgaggg cattctcgac gcgtgcctgg acgcgtgcgg
     2521 cccaggagtc gaactcctgg ctcacgccga acttccgtcg gcgatatcga gcggcttcag
     2581 cgactctccc tcgaggatgc gcaggcgggc atcggcatcg tcgagttgcg actggcaatg
     2641 gcgaagcagc gcggcgccac gccgatacgt cgtcagcgat tcctcgagcg tgagccgacc
     2701 actctccatg cgctcaagca ccgcgtcgag ttcggtcagt gctgcttcaa atggcaggtc
     2761 tgcggagacc tggtcggcgt cgtcgatgcg tttgtttttc acaatggaat gcgcgggtca
     2821 gtcagattcg gcggaaggct catgccaacg ccgattgcgg gtgacccggc tggtgcgttg
     2881 gggctagaat tatatcgctt tcaatcatct ggccttgctt ttcgggggag gggcgatgtc
     2941 cgacatcggc aattcggcgc agttgtcgcc ggcgacaacg cagttgccgg tcgagtggta
     3001 cttcgacgag cgcgttttcg cgcttgagat gaagcacctg tttgccgacg ggccaggcta
     3061 tctcggccac gagttaatgg tgccggaaat ccacgattac cggacacttg agtggcagga
     3121 ccacgggcgc atgctggtcc gccaacccga cagcgtctac gcaatgtcga acgtttgccg
     3181 gcaccggcag gcgatcatgc tgcagggcgc cggcaatgcc cgcaacatcg tctgcccgat
     3241 ccaccggtgg acctacggca tggacggtgc gctgatgggg gcgccgcatt ttccggcgaa
     3301 tccctgcctc aatctcgaca agcaaaagct tgaatcctgg cagggcctcc tgttcggggg
     3361 gccgcgctcg gcgaaccgtg atctggccgg catgcaggcg ctcaccgact tcgacttttc
     3421 cgggtatcag ctcgatcgtg tcgaacagca cacttgtcac tacaactgga agaccttcat
     3481 cgaggtctac ctcgaggact accacgtcgc gccgtttcat ccggggctcg gcaacttcgt
     3541 tacctgcgac gacctgagct ggcagtttgg tgactggtat tcggtgcagc gtgtcggcat
     3601 tacttcgctg gtcaagtcgg gctcggcggc ctatgggcgt tggcaacagg ccgtccgcga
     3661 ctattcgctg gcgtctgggc gggcccctcg gcagggcgcg gtctggctga cctatttccc
     3721 gaacgtgatg gtcgagtggt atccgcacgt actggtggtt tcttcgctga ttccgcaggc
     3781 cgtcgatcgc acgctcaacg tcgtcgagtt ctactatccc gaggaaatag ccgccttcga
     3841 gcgcgaattc atcgaggccg agcaggccgc gtacatggaa acggccctcg aagacgacga
     3901 catcggggag cgcatggacc gggggcgtcg ggcgctgatg cgccgcggcc taggacaggc
     3961 cggtccctac cagtcgccga tggaagacgg catgcagcac ttccatgagt tctaccggcg
     4021 aatcatcgag ccccgactct gagatggcgc gcttatcggg gcgtaagccc cgtcagccgg
     4081 cagccgccgg cgcgacgtgc tgagtgcccg gcagtcattg tggatgctgg cggcgagttt
     4141 cggtttcgcc tgcatggggg tctgcgtcaa gctcgccgcc gcgcgctatg cgatcgccga
     4201 gatcgtcttc tggcgcagcg tcatcgccgt cagcgtttcg gcggtattca tgcacggcta
     4261 cggtctcgcc ttcagcacac cgcaactgcg ctggcagatc ggccgcggta
//
LOCUS       DATDAA010000128         4403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_218902,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000128 DATDAA010000000
VERSION     DATDAA010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218902"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2515
                     /gene="dnaE"
                     /locus_tag="VIS73_04090"
     CDS             <1..2515
                     /gene="dnaE"
                     /locus_tag="VIS73_04090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY5762368.1"
                     /translation="STVRESRRAEYEARLAFECQTIVQMGFPGYFLIVADFINWAKQN
                     GVPVGPGRGSGAGSLVAYSLGITDLDPLAYALLFERFLNPERVSMPDFDVDFCQDNRW
                     RVIDYVRRKYGEQAVSQIATFGTMSSKAVVRDVGRVLELPYSLCDRISKLIPVVQNKP
                     VSLADALEQEPQLKEMLADEHDGEALQQLFSLAGKLEDMARNVGMHAGGVLIAPGKLT
                     DFCPLYQQDTRDAVPVSQFDKDDVEKVGLVKFDFLGLRNLTIIQLAVEYIEQLSGERI
                     DLLSLRFDDPAAYQILKDANTTAIFQVESEGMKKLLKKLEPDRFEDIIAVLALYRPGP
                     LGSGMVDDFILRKKGRQTIDYFHPDLKSSLEPTYGVIVYQEQVMQISQIIGGYTLGGA
                     DMLRRAMGKKKPEEMAKHRSTIAEGAAKKCYDPALAEQLFDLMTKFAEYGFNKSHTAA
                     YAVVTYQTAWLKAHHTAAFMAATLSSDMDNTDAVKGFIDDTVRNRVVILGPDINASGY
                     RFVPVDRDTIRYGLGAVKGTGENAVNAIIEARAAGPFVDLFDFCRRVDKRLVNRRAVE
                     ALIRSGAFDSLDDNRARLLASVGIALDAAEQAARDALQGGLFDGVGDAPALTPALIDA
                     PEWDQRQRLLEEKAALGFYFSGHLFEGYREEISRWCRSDIAALEPRKESVTVAGIVID
                     LRMQMTRRGKMAVVTLDDGRGRIEVTVFSELFESVRERIRIDQPLIVEGKVSRDDFRD
                     GLRLVADRAMSLAEARGRFAKALRISLNGKARDPAAAERLRQELSPYIGGECPVRLVY
                     RNDSASCELSMGADWQVRLEDELLAGLRSWLSPADVEIL"
     gene            2613..3116
                     /locus_tag="VIS73_04095"
     CDS             2613..3116
                     /locus_tag="VIS73_04095"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEY5762369.1"
                     /translation="MLDREGFRPNVGVILCNRKNQVFWGKRIKEHSWQFPQGGMKRGE
                     SPEQAMYRELYEEVGLRPEHVRILGRTRDWLRYEVPPHWVRREWRGSYRGQKQIWFLL
                     RLTGRDSDISLRASDHPEFDAWRWHEYWIPLETVIEFKREVYRLALDELERYLGADRR
                     RQHLESV"
     gene            3158..3778
                     /gene="coq7"
                     /locus_tag="VIS73_04100"
     CDS             3158..3778
                     /gene="coq7"
                     /locus_tag="VIS73_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEY5762370.1"
                     /translation="MSPIDDAIGLFDRALRAVAGVAASAEKFPAGNDAALTADERRQV
                     ISMMRVNHAGEVCAQSLYLAQALAARDPALREHFLRSAEEESAHLAWMRARLAELGGR
                     RSLLDPLWFAGAAGIALAAAAAGDRVSLSFLHETEQQVGAHLDRHLRRLPDADRRSQA
                     MLTRMRDDELTHSAAAKARGAPDMPAPGRAAMRAAAGVMTGIAAYV"
     gene            complement(3836..4264)
                     /locus_tag="VIS73_04105"
     CDS             complement(3836..4264)
                     /locus_tag="VIS73_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY5762371.1"
                     /translation="MECTVRWLNEGMSFVATTGSGHLVTMDGAPEAGGSNLAPRPMEL
                     LLAGTGGCSAFDVVLILRRGRHDVQDCVVRLEAARAEKEPKVFERIHFTFTVTGRKLK
                     REAVERAVSLSKEKYCSASIMLAKTAEITHSVEVVDLDDL"
BASE COUNT          765 a         1345 c         1470 g          823 t
ORIGIN      
        1 ctcgacggtc cgcgagagca ggcgcgccga gtacgaggcg cggctcgcct tcgagtgcca
       61 gaccatcgtg cagatgggct tccccggcta ctttctgatc gttgccgact tcatcaactg
      121 ggcgaagcag aacggcgttc cggtcggtcc cggccgcggt tcgggcgcgg gttcgctggt
      181 cgcctacagt ctgggcatca ccgacctcga tccgctcgcc tacgcgctgc tgtttgagcg
      241 cttcctcaat cccgagcggg tgtcgatgcc cgacttcgac gtcgacttct gccaggacaa
      301 tcgctggcgc gtgatcgatt acgttcgtcg caagtacggc gagcaggcgg tgtcgcagat
      361 cgctaccttc ggcacgatgt cgtcgaaggc cgtcgtgcgt gacgtcggcc gcgtgctcga
      421 actgccgtac tcgctgtgcg accggatttc caagctgatt ccggtggtgc agaacaagcc
      481 cgtgtcgctc gccgacgcgc tcgagcagga gccgcagctc aaggagatgc tcgccgacga
      541 gcacgacggc gaggcgctgc agcagctctt ctcgctggcc ggcaagcttg aggacatggc
      601 gcgtaacgtc ggcatgcacg ccggtggcgt gctgatcgcc ccgggcaagc tcaccgattt
      661 ctgcccgctc taccagcagg acacgcgcga cgcggtgccg gtgtcgcagt tcgacaagga
      721 cgacgtggag aaggtcggcc tggtcaagtt cgacttcctt ggcctgcgca acctgaccat
      781 cattcagctc gccgtcgagt acatcgagca gttaagcggc gaacgcatcg acctcctgtc
      841 cttgcgtttc gatgatccgg cggcctacca gattctcaag gacgccaata ccacggcgat
      901 cttccaggtt gaatcggagg gcatgaagaa gctgctgaag aagctcgagc ctgatcgttt
      961 cgaagacatc atcgccgtgc tggcgctcta ccgcccgggc ccgctgggct cggggatggt
     1021 cgatgacttc attttgcgca agaagggccg gcagacgatc gactacttcc acccggacct
     1081 caagtcgtcg ctcgaaccca cctacggcgt catcgtctac caggaacagg tgatgcagat
     1141 ctcccagatc atcgggggct atacgcttgg tggcgccgac atgctgcggc gagcgatggg
     1201 caagaagaag cccgaggaga tggccaagca ccgctcgact atcgccgagg gtgcggcgaa
     1261 gaaatgctac gacccggcgc tcgccgagca gctgttcgac ctgatgacca agttcgccga
     1321 gtacggcttc aacaagtcgc acacggctgc ctatgcggtg gttacctacc agaccgcgtg
     1381 gctgaaggcg caccacacgg cggcgttcat ggcggcgacg ctgtcttccg acatggacaa
     1441 caccgacgcg gtcaagggct tcatcgatga caccgtccgc aatcgggtcg tgatcctcgg
     1501 cccagacatc aacgcctcgg gataccgctt tgttcccgtc gaccgggaca cgatccgcta
     1561 tggtctcggc gccgtgaagg ggaccggcga gaacgccgtg aatgcgatca tcgaggcacg
     1621 cgccgcgggg ccgttcgtcg atctctttga cttctgccgc cgcgtcgaca agcgcctcgt
     1681 caatcggcgg gcagtcgagg cattgattcg ttcgggggcc ttcgattcgc ttgacgacaa
     1741 tcgtgcgcgc ctgctggcaa gtgtcggtat tgcgctcgat gcggccgagc aggcggcgcg
     1801 cgacgcgctt cagggtgggc tgttcgacgg tgtcggcgat gcgccggcgc tgacgccagc
     1861 cctgatcgac gcgccggaat gggaccagcg ccagcgtctg cttgaggaaa aggcggcgct
     1921 aggcttctat ttctctgggc atctctttga gggctatcgc gaggaaattt ctcgctggtg
     1981 ccgtagcgat attgcggcgc tggagccgcg caaggagtcg gtgacggttg ccggcatcgt
     2041 catcgatctg cgcatgcaga tgacgcgccg cggcaagatg gccgtcgtta cactcgacga
     2101 cgggcgtggc cggatcgagg tgacggtctt cagcgaactc ttcgaatcgg tgcgcgagcg
     2161 gatccggatc gatcagccct tgatcgtcga aggcaaggtc agccgcgacg actttcgcga
     2221 tggcttgcgg ttggtcgctg atcgcgcgat gtcgcttgcc gaagcgcgcg gtcgctttgc
     2281 caaggcgctg cggatcagcc tcaatggcaa ggcgcgcgac cccgccgcgg cagaacgcct
     2341 gcggcaggag ctgtccccct atataggcgg cgaatgcccg gtgcgactcg tctatcgcaa
     2401 cgactcggcc tcctgcgagc tgtcaatggg tgccgactgg caggtgcggc ttgaggacga
     2461 gttgctggcc gggctgcgca gctggttgtc gccggccgac gtcgagattc tctgaaacgg
     2521 cgcgcttgcg cccgccgcgt gggcgacgaa cttttcaatg cgcgggaagt catggagaat
     2581 cgaaggctga caagcattgg atggatgtgg ttatgctcga ccgtgagggg tttcgcccga
     2641 acgtcggcgt tatcttgtgc aaccgcaaga atcaggtttt ctggggcaaa cggatcaagg
     2701 agcattcctg gcagttcccg caaggcggca tgaagcgtgg cgagtcgccc gagcaggcaa
     2761 tgtaccgtga gctttacgaa gaggtcggtt tgcgtcccga gcatgtccgg attctcgggc
     2821 gtacgcgcga ctggttgcga tacgaggttc cgccgcactg ggttcgccgc gagtggcgcg
     2881 gcagttatag aggacaaaag cagatctggt ttctgctgcg gttgaccggg cgcgattcgg
     2941 acatttcgct gcgcgccagc gatcatcccg agttcgatgc ctggcggtgg cacgaatact
     3001 ggattccgct ggaaaccgtt atcgagttca agcgcgaggt ctacaggctc gcactcgatg
     3061 aacttgaacg ttatctcggc gccgatcggc gccgccagca cctggaaagc gtttgaggcg
     3121 tgttgggcgc cgccggtgaa ccaggcgaaa gggctgtatg agtcccattg acgatgcaat
     3181 cggcctgttt gatcgggcct tgcgggcggt ggccggcgtg gcagcgtcgg ccgagaagtt
     3241 tccggcgggc aacgatgcgg cgctaaccgc cgacgagcgt cggcaggtca tttcgatgat
     3301 gcgcgtgaat cacgccgggg aggtttgcgc ccaatcgctc tatttggctc aggccttggc
     3361 agctcgcgat ccggcgttgc gcgagcactt cctgcgcagt gccgaagagg agtccgcaca
     3421 cctggcatgg atgcgcgcgc gtctcgccga acttggcggg cgaaggagcc tgcttgaccc
     3481 gttatggttc gccggcgctg cggggatcgc gctggcagcg gcggcggccg gggaccgcgt
     3541 cagcctttcg ttcttgcacg agaccgaaca gcaggtaggc gcccatctcg atcgccactt
     3601 gcggcgcctg ccggacgccg atcgacgttc gcaggcgatg ctgacgcgca tgcgtgacga
     3661 cgagttgaca cattcggcag cggcgaaagc ccgcggcgca cccgacatgc ccgcaccggg
     3721 ccgcgcggcg atgcgggcgg ctgctggcgt gatgaccgga atcgcggcct acgtctgagc
     3781 gccccggggc cgggcttcgc ccccgtgggt ggcaaggaaa ctcggggcgg gccttctaga
     3841 gatcgtcgag atcgacgact tcgacgctgt gggtgatttc ggcggtcttc gcgagcatga
     3901 tcgacgccga acagtacttc tctttggaaa gggacacggc gcgctcgacg gcctcgcgct
     3961 tcagctttcg gccggtgacc gtaaacgtga agtgaatccg ctcgaagacc ttgggttctt
     4021 tttcggcgcg cgcggcttcc aatcgtacga cgcagtcctg aacgtcatgg cggccgcgac
     4081 ggaggatcaa gacaacatca aaggcggagc atccgccggt tccggcgagc agcagttcca
     4141 tcggccgcgg cgcgagattg ctgccgcccg cctcaggtgc gccgtccatc gtgacgagat
     4201 ggccactccc ggtcgtcgcg acaaacgaca tgccttcgtt gagccagcga acggtgcatt
     4261 ccatcttgcc ttccttcgag cgagtctttt cggcggcggg attctagcag ctacccattg
     4321 cgtcacggcc gcgcggacaa cttgccgtgg cgcaacgcaa ccaatgctgc gatgcagcaa
     4381 acaagccgcg gcgcaccgaa agt
//
LOCUS       DATDAA010000129         7239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_498616,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000129 DATDAA010000000
VERSION     DATDAA010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498616"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="VIS73_04110"
     CDS             <1..1331
                     /locus_tag="VIS73_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY5762372.1"
                     /translation="IRIVGAGGRVLAERRVGRIQFRGPSATQGYFENPAATAALFDGD
                     WLNTGDLGYLADGELFVTGREKDLIIRGGHNVYPQELEEAVGRIDGVRAGNVAVFPAT
                     DRQLGTEALVVLAETREAAEAHEGIRAQVEQLAVDLTGLPPDDVVLAPPGTVLKTSSG
                     KLRRTACRAAYERGELLRRPRSVRRQMLTLGLGALRTRLRGGLRSLAGTLWPLWAWTV
                     FSLLAPPVWLTIIAAPAVCQRRAVARAGARLALLATGISPRVEGLEHLAGDAPAVIVA
                     NHASYLDGLLLTAVLPPRFAFVAKQELLGHPLSGTPLRRLGAAFVERFDAARGIEDTQ
                     AIEARLAAGDSLAFFAEGTFRRAPGLLPFRMGAFVLAARTRTAVVPVTLRGTRRVLPD
                     TNRWPRFAALSVTIHAPIRPAGSDWEAALALRDGARRVILAQVGEADAGV"
     gene            1815..3083
                     /gene="urtA"
                     /locus_tag="VIS73_04115"
     CDS             1815..3083
                     /gene="urtA"
                     /locus_tag="VIS73_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HEY5762373.1"
                     /translation="MQRRNFIKATAMTVALAAAGFVSSPTLAADTIKVGILHSLSGTM
                     AISETALKETALMTIEEINAKGGVMGKKLEPVVVDPASNWPLFAEKARQLLTQDKVAV
                     VFGCWTSVSRKSVNPVFKELNGLLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYLM
                     SKDGGSAKRWVLLGTDYVYPRTTNKILRAFLKSKGVADADIMEDYTPFGHSDYQTIIA
                     KIKKFASEGKKTAVVSTINGDSNVPFYKELGNAGLKATDVPVVAFSVGEEELRGVDTK
                     PLVGHLAAWNYFQSIKNPTNDAFIKMYQGWAKKQKLPNAETVVTNDPMEATYIGIHMW
                     KQAVEKAKTTDVDKVIAAMGGQEFMAPSGFKIKMDEKNHHLHKPVFIGEVKADGQFNV
                     VWKTKGPIKAQPWSPYIPGNDKKKDEPEGK"
     gene            3165..4691
                     /gene="urtB"
                     /locus_tag="VIS73_04120"
     CDS             3165..4691
                     /gene="urtB"
                     /locus_tag="VIS73_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEY5762374.1"
                     /translation="MKSLRHLLLGLCCAFAVGTFAAATHAAVDPALVEQLAADDSDLK
                     IEAIQKIAETADPEAVTLLQSMADGTLLASDGSEIMINNRIRGELANALAGLRLFDAS
                     ADVRRAAARELQTNVGIELVRILARARDKETVPDIKDLMTAAYAQASLASPEASIRLE
                     AVRTLGEFASPNTRNLLLPLTRTEGEPDQSVRAAALEALKVIERRLLVGESLGSVFTG
                     LSLGSILLLAALGLAITYGVMGVINMAHGELLMIGAYATYAIQGVFRSHFPEWIDAYV
                     LAAIPVAFFAAALVGIVLERTVIRHLYGRPLETLLATWGLSLILIQGARVLFGAQNVE
                     VANPVWMSGGVELMAGLVLPWNRIAIIAFSLLVLLLVWLLMTRTRLGMFVRAVTQNRQ
                     MAGCVGVHTGKVDTLAFGLGAGIAGLGGVALSQIANVGPDMGQGYIVDSFMVVVLGGV
                     GQLAGAVWAALGLGVFTKFLEGWAGAVIAKIVVLVFIIIFIQKRPQGIFALKGRFVES
                     "
     gene            4688..5797
                     /gene="urtC"
                     /locus_tag="VIS73_04125"
     CDS             4688..5797
                     /gene="urtC"
                     /locus_tag="VIS73_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEY5762375.1"
                     /translation="MRVPISVRIFHATPRGGWIALAVAALLVFVLLPVLHLALPETHP
                     LHVSTYFITLAGKIMCYAIVAVAMDLIWGYAGILSLGHGLFFALGGYAFGMYLMRQIG
                     RDGSYQSDLPDFMVFLDWKELPWYWIGSDSFLWALLLVVVVPALVAFIFGFFAFRSRI
                     KGVYFSIITQALTFAAMLLFFRNETGFGGNNGFTDFKRILGHDVTSPEMRVILFALSA
                     ALLMTTLVLGRYLVTSKFGRVLAAIRDAESRVMFLGYDPLWYKLGIWTLSAVLCALAG
                     ALYVPQVGIINPGEMAPANSIEIAIWVAVGGRGTLIGPVIGAGIVNLSKSFFTQTFPD
                     YWLFFLGALFVAVTLWLPDGVAGLWKRWQSRRSPS"
     gene            5860..6624
                     /gene="urtD"
                     /locus_tag="VIS73_04130"
     CDS             5860..6624
                     /gene="urtD"
                     /locus_tag="VIS73_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEY5762376.1"
                     /translation="MRSGEVDVTHKVILYLEGISVSFDGFKALNDLNLAIDAGELRCI
                     IGPNGAGKTTMMDVITGKTRPDSGRCFFGQTMDLTTMREAEIAHAGIGRKFQKPTIFE
                     NLSAFENLELALRTDKRAWISLFAWLGDDQRDRIADMLRLIRLDEEADRPAGLLSHGQ
                     KQWLEIGMLLVQEPKLLLLDEPVAGMTDDETERTGELFKSLAGKHSLVVVEHDMAFIE
                     QLGGKVTVLHEGSVLAEGDLATVQSDERVIEVYLGR"
     gene            6635..>7239
                     /locus_tag="VIS73_04135"
     CDS             6635..>7239
                     /locus_tag="VIS73_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5762377.1"
                     /translation="MLLVDNLNQYYGGSHILRDVAFEAPPGKVTALLGRNGVGKTTLL
                     KCLMGLVPAKTGSIRFAGQEIAALPSHLRVRAGIGYVPQGREIFPRLTVEENLLMGMA
                     SLPRTSRLPERIFELFPVLKSMLRRRGGDLSGGQQQQLAIGRALALNPRLLILDEPTE
                     GIQPSIIKEIERAIRVVASSRPKIDEAKKAAERQEIMEAIK"
BASE COUNT         1230 a         2288 c         2341 g         1380 t
ORIGIN      
        1 acatccgtat tgtcggcgca ggcggtcgcg tcctggctga acgacgcgtc ggtcgcatcc
       61 agtttcgcgg cccctcggca acgcagggct acttcgagaa tccggccgca accgcggcac
      121 tctttgacgg cgactggctc aacaccggcg atcttggata cctcgccgac ggcgagctgt
      181 tcgttaccgg ccgcgagaag gacctgatca tccgcggcgg gcacaacgtc tatccgcagg
      241 aactcgagga agcggtcggg cgtatcgacg gcgtgcgcgc tggcaacgtg gcggtgtttc
      301 cggccactga ccggcagctc ggcaccgaag cgctggttgt cctcgccgaa acccgcgagg
      361 cggccgaggc gcacgagggc atccgggcgc aggtcgagca gctcgccgtc gacctgaccg
      421 gtctgccgcc cgacgatgtg gtgctcgcgc cgcccggtac cgtgctcaag acatcgagcg
      481 gcaagctgcg ccgcaccgcg tgccgtgcgg cctacgagcg cggcgaactg ctgcgccggc
      541 cccgttcggt acggcggcag atgctcacac ttggactggg cgcgctgcgc acccgcctgc
      601 gtggcgggct gcgcagtctc gccggcacgc tctggccgct ctgggcgtgg acggtgtttt
      661 ccctgctggc gcccccggtc tggctgacga tcatcgccgc acccgccgtc tgccaacgcc
      721 gcgcggtggc gcgtgccggt gcccgtctgg cattgctggc gactggtatc tcgccgcgcg
      781 tcgaaggtct cgaacacctg gctggcgacg cgcccgccgt catcgtcgcc aaccacgcta
      841 gctacctcga cggcctgctg ctgaccgccg tgttgccgcc gcgctttgcc ttcgtcgcca
      901 agcaggaact gcttggccat ccgctgtcgg gcacgccatt gcggcgtctc ggcgcggcct
      961 tcgtcgagcg cttcgacgcg gctcgcggca tcgaggacac gcaggcgatc gaggcgcgct
     1021 tggcggcggg tgattcgctg gccttctttg ccgaaggcac cttccgccgc gcgcccgggc
     1081 tgctgccgtt tcgcatgggg gcattcgtgc tggcggcgcg cacgcggacg gcggtcgtgc
     1141 cggtgaccct gcgtggcacg cgccgcgtgc tgcccgatac caaccgctgg ccgcgcttcg
     1201 cggcgctgtc ggtgacgatc cacgcgccga ttcggccggc cggcagcgac tgggaagcag
     1261 cgcttgccct gcgtgatggc gcgcgccggg tgatcctggc gcaggtcggc gaagcggatg
     1321 ccggcgtgtg aggccggcac tcttcagatc gggctgcatg caacctcgtg cggaagtcga
     1381 cgattcaatg tcgcattgcc gaagccagtc cctccaccat caatgaaacg ctggctgacg
     1441 gcacctaggg cgtttcacgt gagcctgctg aaactcgtcg tgccttgagc ggtcgctgcg
     1501 ggtttcccgc cggtcgacgg ccgatgcacc aaatcatcgc ggccggggtt ggggctgcgc
     1561 caatcaggtg cccgcctccg cacagttgat gtgcttaacc acccgtaact gccgagcgac
     1621 agagcggcct tcgcctcctc accccgcggg tgcagcggtg cgctggggct tgttctcccg
     1681 gtctttctga ccaggcgctg caagtgtcgg ttggcgcaat tgcgacaacc gatctgccgg
     1741 ttggcacgct cctcgctgtt aagggttcgc agtcctctcc atttctgccc cttaacctac
     1801 gaggagtctt gctcatgcag cgtcgcaatt tcatcaaggc caccgcaatg acggtcgcac
     1861 tggctgcggc cggatttgtt tcgagtccga cccttgccgc cgacacgatc aaggtcggca
     1921 tccttcattc gctgtcggga acgatggcga tttccgagac ggcgctaaag gaaacagcgc
     1981 tgatgacgat cgaggagatc aacgccaagg gtggcgtgat gggcaagaag cttgagcccg
     2041 ttgtcgtcga cccggcgtcg aattggccgc tgttcgccga gaaggcacgc cagctgctga
     2101 cccaggacaa ggtggcggtg gtcttcggct gttggacgtc agtctcgcgc aagtcggtga
     2161 acccagtgtt caaagagctc aacggcctgc tgttctaccc ggtgcagtac gagggtgagg
     2221 aactcgagaa aaacgtcttc tacactggcg cggcgcccaa ccagcaggcg atcccggcgg
     2281 tcgaatacct gatgagcaag gacggcggct cggcgaagcg ctgggttctg ctcggcactg
     2341 actacgtcta cccgcgcaca accaacaaga tcctgcgtgc cttcctcaag agcaagggtg
     2401 ttgccgacgc ggacatcatg gaggactaca cgcccttcgg tcacagcgac taccagacca
     2461 tcatcgctaa gatcaagaag ttcgccagcg aaggcaagaa gaccgccgtt gtgtcgacca
     2521 tcaacggtga ctccaacgtg cccttctaca aggaactcgg caatgccggc ctgaaggcaa
     2581 ccgatgtgcc ggtggtggcc ttctcggtcg gcgaggaaga gctgcgcggc gtcgatacca
     2641 agccgctggt cggccatctg gcggcatgga attacttcca gtcgatcaag aacccgacta
     2701 acgacgcgtt catcaagatg taccagggct gggcgaagaa gcagaagttg ccgaacgccg
     2761 agaccgtcgt caccaacgac ccaatggagg ccacctacat cggcatccac atgtggaaac
     2821 aggcggtcga gaaggccaag accaccgatg tcgacaaggt gatcgcggcg atgggcggcc
     2881 aggagttcat ggcgccgtcg ggcttcaaga tcaagatgga cgagaagaac caccacctgc
     2941 acaaaccggt gttcattggc gaagtaaagg cggatggcca gttcaacgtc gtctggaaga
     3001 ccaagggacc gatcaaggca cagccgtggt cgccgtacat ccccggcaac gacaagaaga
     3061 aggacgagcc ggaaggcaaa taagccgaca ggcgattcct ttgtgaaacg gggcagcgcg
     3121 ctgggttgtt ccaggcgcgc tgcccgacaa gcaaaccccg acctatgaaa tctctccgtc
     3181 atctgctgct gggcctgtgc tgcgcgtttg ctgtcggcac cttcgccgcg gcgacgcatg
     3241 ctgccgtcga tccggcgctg gtcgagcaat tggctgccga tgattcggac ctcaagatcg
     3301 aggcgatcca gaagatcgct gaaaccgctg accccgaggc ggtgacgctg ctgcagtcga
     3361 tggccgatgg cacactgctg gcgtcggatg gcagcgaaat catgatcaac aaccgcatcc
     3421 gcggcgaatt agctaatgcc ttggcggggc tgcgcttatt cgatgccagc gcagacgttc
     3481 ggcgagcggc agcccgtgag ttgcagacca acgttggcat cgagctggtg cggatcctcg
     3541 ctagggcgcg cgacaaggaa accgtccccg acatcaagga cctgatgacg gcagcctacg
     3601 cgcaggccag tctggccagc cccgaggcgt cgatccggct cgaagcggtt cgcacgctgg
     3661 gagagtttgc cagccccaat acccgcaatt tgctgctgcc gctgaccagg accgaaggtg
     3721 aaccggacca gtccgttcga gcggcagccc tcgaggcgct aaaggtcatc gagcggcgcc
     3781 tgttggtcgg tgaatcgctg ggcagcgtgt tcaccgggct ctcgctcggc agcatcctgc
     3841 tgctcgcggc gctcggtttg gcaatcacct acggtgtgat gggcgtcatc aacatggcgc
     3901 acggcgaatt gctgatgatc ggcgcctacg caacctacgc aatccaaggc gtctttcgca
     3961 gccatttccc ggaatggatt gatgcctacg tgctggcggc gattccggtg gctttcttcg
     4021 ccgccgcgct agtcggcatc gtgctcgaac gcacggtgat ccgtcatctt tacggccggc
     4081 ccctggaaac gctgctcgcg acctggggcc tgtcgttgat cctgattcag ggtgcgcgcg
     4141 tgcttttcgg cgcgcagaac gtcgaggtcg ccaacccggt ctggatgtcg ggcggcgtcg
     4201 agctgatggc tggactggta ctgccctgga accgcatcgc gatcattgcc ttttctttgc
     4261 tcgtcttgtt gttggtctgg ctgctgatga cgcggacacg gcttggaatg ttcgttcgcg
     4321 cggtgactca gaatcgccag atggccggct gcgtcggcgt tcatacaggc aaggtcgata
     4381 cgctcgcgtt cggtctcggt gccggcatcg ccgggctcgg tggcgtcgcg ctgtcgcaga
     4441 tcgccaacgt cggcccggac atggggcagg gctacatcgt cgattcgttc atggtggtcg
     4501 tgctcggcgg tgttggccag ctggcgggcg cggtctgggc tgcgctgggg ctgggcgtct
     4561 tcaccaagtt tctcgaaggc tgggccggcg ccgtgatcgc caagatcgtc gtgctggtct
     4621 tcatcattat cttcattcag aagcgccccc agggaatctt tgccctcaag gggaggttcg
     4681 tcgagtcatg agggtgccga tctcggtccg gatctttcat gccacgccgc gcggtggctg
     4741 gattgcgctg gctgtcgcgg cgcttctggt tttcgtcctg ctgccggtgc tgcatctggc
     4801 attgcccgaa acccacccgc tgcatgtatc gacttatttc atcacgctgg ccggcaagat
     4861 catgtgctac gcgatcgtgg cggtggcgat ggatctgatc tggggttatg cagggattct
     4921 ctcgcttggc cacggtctct tcttcgcgct cggcggctac gccttcggca tgtacctgat
     4981 gcggcaaatc ggccgcgacg gctcctacca gtcggatctc ccggacttca tggtcttcct
     5041 cgactggaag gaactcccct ggtactggat cggttcggat tcgttcctct gggccctgct
     5101 gctggtggtc gtggtgccgg cgctggtggc cttcatcttc ggtttctttg ccttccgctc
     5161 gcggatcaag ggcgtctact tttcgatcat cacccaggcg ctgacctttg cggcaatgct
     5221 cttgttcttc cgcaacgaaa cgggctttgg cggcaacaac ggcttcactg acttcaagcg
     5281 catcctcggc catgacgtca cgtcgcctga gatgcgtgtg atcttgttcg cactttccgc
     5341 cgccctgctg atgacgacgc tggtcctcgg gcgctatctt gtcaccagca agttcggtcg
     5401 cgtgctggcg gcgatccgcg atgccgaatc gcgcgtcatg ttcctcggct atgacccgct
     5461 ttggtacaaa ctcggcatct ggacgctgtc ggctgtcctc tgcgcgctcg ctggcgcgct
     5521 ctacgtgccg caggtcggga tcatcaatcc cggcgagatg gcgccagcga actcgatcga
     5581 gatcgctatc tgggtcgcgg tcggcggtcg cggcaccctg atcggcccgg tgatcggcgc
     5641 cggcatcgtc aatctttcga agagcttctt cacgcagacc tttccggatt actggctgtt
     5701 cttcctcggt gcgctgttcg tcgcggtgac gctgtggctt cccgacggtg tcgcgggatt
     5761 gtggaagcga tggcagtcgc ggcggagccc atcgtgagtg cggtcaccga aaccgccaat
     5821 gagcggactt ggagcggcag cgcctcgacc ggccacgcgc tgcgttccgg tgaggtggac
     5881 gtcacgcaca aggtcatcct ctacctcgag gggatctccg tcagcttcga cggatttaag
     5941 gcgctcaacg acctcaacct cgcgatcgac gccggcgaac tgcgctgcat catcggtccg
     6001 aacggtgccg gcaagacgac gatgatggac gttatcaccg gcaagacgcg gcccgattcc
     6061 gggcgctgtt tctttggcca gacgatggat ctgaccacga tgcgcgaggc cgagatcgcc
     6121 cacgccggaa tcggccgtaa gtttcagaag ccgaccatct tcgagaacct gtcggccttc
     6181 gagaacctcg agctggcgct gcgcaccgac aagcgcgcgt ggatcagcct gttcgcctgg
     6241 ttgggcgacg atcagcgcga ccgcatcgcc gacatgctgc gcttgatccg gctcgacgaa
     6301 gaagccgacc ggcccgccgg cctgctctcg cacggccaga aacagtggct ggagatcggc
     6361 atgctgctgg tgcaggaacc caagctgctg ctgctcgacg agccggtcgc cggcatgacc
     6421 gacgacgaga cagaacgcac aggcgaactc ttcaagtcgc tcgcgggcaa gcactcgctg
     6481 gtcgtcgtcg agcacgacat ggccttcatt gaacaactcg gcggcaaggt gaccgtgctg
     6541 cacgagggct cggtactcgc cgagggcgat ctcgccacgg tgcagagcga cgaacgcgtc
     6601 atcgaagtct atttgggccg atagcggagc gcgcatgctg cttgtcgaca acctcaacca
     6661 gtactacggc ggcagccaca tcctgcgcga cgtcgctttc gaggcgccgc cgggcaaggt
     6721 cacggcgctg ctcggtcgca acggggtggg caagacgacc ctgctgaaat gcctgatggg
     6781 gctggtgccg gcgaagaccg gcagcatccg gttcgctggc caggaaattg cagccttgcc
     6841 ctcgcatctg cgtgtgcgag ccggcatcgg ctacgtgccg caggggcgag aaatcttccc
     6901 acggctcacg gtcgaagaga acctgctgat gggcatggcc agcctgccgc gtacgagccg
     6961 cctgccggaa cgcatcttcg agctgttccc ggtgctgaaa tcgatgctgc gtcgtcgcgg
     7021 cggcgacctc tccggtggcc agcagcaaca gttggcgatc ggtcgtgccc tggcgttgaa
     7081 tccgcgcctg ttgatcctcg acgagccgac cgaaggcatc cagccatcga tcatcaagga
     7141 aattgaacga gcgattcgcg tcgtcgccag cagccggcca aagattgatg aggcaaagaa
     7201 ggccgccgaa cggcaggaaa tcatggaggc gatcaagaa
//
LOCUS       DATDAA010000130         6855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_114579,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000130 DATDAA010000000
VERSION     DATDAA010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114579"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1255
                     /gene="dusB"
                     /locus_tag="VIS73_04140"
     CDS             233..1255
                     /gene="dusB"
                     /locus_tag="VIS73_04140"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY5762378.1"
                     /translation="MEFLGFSLRNNLFVAPMAGVTDRPFRQLCKKMGAGLAVSEMVTS
                     NSLLYGSTKTLRRANHEGEVAPVSVQIAGADPQMMAEAAKHNVDNGAQIIDINMGCPA
                     KKVCNVAAGSALMKDEALVGRILEAVVNAVPRTPVTLKTRTGWDTSDRNAPRLLKIAE
                     ESGVRALAVHGRTRCQQYTGHAEYETIRAIKHEAKIPVIANGDIDTPEKAKLVLDTTG
                     ADALMIGRAAQGRPWLFREIEHYLATGTHLPPPHPLEIRAILLDHLEDLYGFYGTEVG
                     VRIARKHIGWYTKGLSASAAFRFRLNQLPSIAEQRDAIDTYFESLAAQGAPIARAGGD
                     SEELAA"
     gene            1252..1491
                     /locus_tag="VIS73_04145"
     CDS             1252..1491
                     /locus_tag="VIS73_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEY5762379.1"
                     /translation="MSRGDDIAGCIRRSIDRYFRDLDGEKPAAIYPMVISCVERPMLE
                     AVMRHAAGNQSLAAEMLGINRNTLRKKLLEHDLIA"
     gene            1562..3154
                     /gene="purH"
                     /locus_tag="VIS73_04150"
     CDS             1562..3154
                     /gene="purH"
                     /locus_tag="VIS73_04150"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY5762380.1"
                     /translation="MTIRQALISVSDKSGVLEFAQGLAAQGVKLLSTGGTAKMLRDAG
                     LAVTEIGDYTGFPEMLDGRVKTLHPKVHGGILARRDTPEHMATIAAHDIPCIDLVCVN
                     LYPFAATVAKPGVTLEDAIENIDIGGPAMVRSSAKNYAGVAIVTDPADYADLLAEMKA
                     NDGALELVTRFNLAKKAFTHTARYDGMIANWLTGLADGAEAKPDAAAPVPSVFPDRLQ
                     LAFDRVETLRYGENSHQSAAFYRDTNSVQGSIAAYHQLQGKELSYNNIADADAAWECV
                     KSFDAPCCVIIKHANPCGVAIDGTLLAAYEKAFKTDSTSAFGGIIAFNGEVGIDVVEA
                     MSARKHFVEVLIAPAFTAEAKTALAGKTNLRVLEVPLSKTLNALEFKRVGGGLLVQTP
                     DAFTAQADGLKIVTKKQPTREQIEDLLFAERVAKFVKSNAIVFCGGGMTLGVGAGQMS
                     RVDSTKIASIKAQHAGLELKGSVVASDAFFPFRDGVDVLAAAGAVAVIQPGGSMRDEE
                     VIAAADEHGLAMVFTGARHFRH"
     gene            3250..3792
                     /gene="pagP"
                     /locus_tag="VIS73_04155"
     CDS             3250..3792
                     /gene="pagP"
                     /locus_tag="VIS73_04155"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: protein motif:HMM:NF008271.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HEY5762381.1"
                     /translation="MALCAALSVAPTAAAEGDGWATRTADHLSGIALDGDTEVFLPFL
                     TYHLRYAYTPEKIASYEESPLGFGIGRGRFLDDGNWAGLFVMGFQDSHSRPQWLAGYG
                     WQKIWNTGPGIRIGLGYTAFLTARTDINHYQPTPAILPLASIGYGNLSIEGTFVPGGT
                     GYGNILFFWAKWRPDHKESP"
     gene            3789..5057
                     /gene="purD"
                     /locus_tag="VIS73_04160"
     CDS             3789..5057
                     /gene="purD"
                     /locus_tag="VIS73_04160"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY5762382.1"
                     /translation="MKLLVIGGGGREHALAWKLAQSTGLQKVYVAPGNAGTAREHELE
                     NVNITDPIALADFAEREKVHLTVVGPEAPLAAGVVNIFRARGLKVFGPTREAAQLESS
                     KDFAKRFMARHQIPTAEFATFSEPKAAHEYVDSKGAPIVIKADGLAAGKGVVVAMTAA
                     EAHQAIDDMLSGNTLGDAGARVVIEEFLSGEEASFIVMVDGKHVLPLASSQDHKRLKD
                     GDLGPNTGGMGAYSPAPVVTPAIHARVMREVIMPTVKGMAADGIPFTGFLYAGLMISP
                     DGTIKTLEFNCRMGDPETQPIMMRLKSDLRTLCEHAVAGTLDKVEAEWDRRVALGVVL
                     AAHNYPATPRKGDAIDGLAQQHDDAHIFHAGTAEQSGQVVTSGGRVLCVTALGDSVKQ
                     AQKRAYEVLAGVHFDGMQYRSDIGYRAVAR"
     gene            5112..6047
                     /locus_tag="VIS73_04165"
     CDS             5112..6047
                     /locus_tag="VIS73_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HEY5762383.1"
                     /translation="MDVIPLFFVLGVAARLIRSDLKVPGAVYELLAIYLLLAIGLKGG
                     VELARQDVLSLLPAVGLVLLMGIALPLLAFPLLKLRFARADAASIAAHYGSVSVVTYA
                     VCATALTTRQLDYEAYFPIFVAILEVPAILIGVLLARIGTGEAGKPTKWGPLAHEIFS
                     SKSVVLLIGGIAIGWIGGPTSIEPLVPLFYTLFKGALCLFLLEMGLVAASRLDDLRRA
                     GAFLVAFGILFPLVGAAVGLACGWLLGFTPGGVALLMTLGASASYIAAPAAMRIAVPE
                     ANPTLSIGAALGITFPFNIMVGIPLYIAIAERLRS"
     gene            6051..6353
                     /locus_tag="VIS73_04170"
     CDS             6051..6353
                     /locus_tag="VIS73_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3240 family protein"
                     /protein_id="HEY5762384.1"
                     /translation="MQNHPKKLLVIIAEAALEKLLVEDILRLGAHGYTVLDARGDGTR
                     GKRQASWEGDSSIRVEVICDEATAERIADHVSETYFTNYAVTLYVADVRVQRSSKF"
     gene            6513..>6855
                     /locus_tag="VIS73_04175"
     CDS             6513..>6855
                     /locus_tag="VIS73_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEY5762385.1"
                     /translation="MQAIVRKTLGLATSAAVALTLSTGALASDKTRYERLGGNPALEA
                     VVGELWATTSADARINHYFAKTKPEVFAGLMVDFLCQAAGGPCQYKGKDMAGAHTGMK
                     RSEADFNALAEN"
BASE COUNT         1217 a         2464 c         2065 g         1109 t
ORIGIN      
        1 tgacctgtgc cccccggcac gccgggcgct ggccgggcag atgatcttcg gccatcgggc
       61 ttaagccgcc ggccaccggc gtgatcgatt cagccgccca agcgtggtgg aaaatccggc
      121 gcgcgtgacc gatgtcgcaa cctgcaagct ggccgtaagg tcggcgtcgg caattgcgtt
      181 agaattccgc ccttccccct gcccgaccat cgctccgaca cggaaatccg ctgtggaatt
      241 tctcggtttt tcccttcgca acaatctgtt cgtcgcgccg atggcgggag tgaccgatcg
      301 gccgttccgc caactctgca agaagatggg cgcgggactt gccgtttccg agatggtcac
      361 ctcgaattcg ctgctctacg gcagcaccaa gaccttgcgc cgcgccaacc acgaaggcga
      421 agtggcgccg gtgtcggtgc agatcgccgg tgccgacccg cagatgatgg ccgaggcggc
      481 gaagcacaac gtcgacaatg gcgcgcagat catcgacatc aacatgggct gcccggccaa
      541 gaaggtctgc aacgtcgccg ccggctcggc actgatgaag gacgaggcgc tggtcggccg
      601 catcctcgaa gcagtggtca atgccgttcc cagaacgccg gtaacactga agacgcgcac
      661 cggctgggat acgtcggacc gcaatgcacc tcgcctgctg aagatcgccg aggaatcggg
      721 cgtgcgggcg ctcgccgtgc acggccggac gcgctgccag cagtacaccg ggcacgccga
      781 gtacgaaacg atccgcgcga tcaagcacga agcgaagatt ccggtaatcg ccaacggcga
      841 catcgacacg ccggaaaagg cgaaattggt gctcgacacc accggcgccg atgcgctgat
      901 gatcggccgc gcggcgcagg gacgaccctg gctgttccgc gagatcgagc actacctcgc
      961 gaccggcacc cacctgccgc cgccgcatcc gctggaaatc cgcgcgatcc tgctcgacca
     1021 cctagaggac ctctacggct tctacggcac cgaggtcggc gtgcgcatcg cgcgcaagca
     1081 catcggctgg tataccaagg ggctgtcggc ttcggcagcc tttcgtttcc gtctcaacca
     1141 gctgccaagc atcgcagagc agcgggacgc catcgacacc tattttgagt ctctcgcagc
     1201 ccagggcgcc cccatcgccc gggccggcgg cgactccgag gagcttgccg catgagccga
     1261 ggcgacgata ttgccggctg catccgccgg tcgatcgacc ggtatttccg cgatctcgac
     1321 ggcgagaaac cggcggcgat ctatccgatg gtaatttcct gcgtcgagcg accgatgctc
     1381 gaggcggtga tgcgccatgc cgcgggcaac cagagcctcg ccgccgagat gctcggcatc
     1441 aaccgcaaca cgctgcgcaa gaagctgctc gagcacgacc tcatcgcctg atgcccgccg
     1501 gcgacctgac gggcatcccc attcaccagt cttccgattc acccattccc tttcgctgac
     1561 catgacgatc cgccaggccc tcatttccgt gtccgacaag tcgggcgttc tcgaattcgc
     1621 gcagggcctg gccgcccagg gcgtcaagct gctgtcgacc ggcgggaccg cgaagatgct
     1681 ccgcgacgcc ggcctcgcgg tcaccgagat cggcgactac acaggcttcc cggagatgct
     1741 cgacggccgc gtcaagacgc tccatccgaa ggttcacggc ggcatcctcg cgcgccgcga
     1801 cacgcccgag cacatggcga ccatcgccgc gcacgacatc ccctgtattg atctggtctg
     1861 cgtaaacctc tacccgttcg cggcgaccgt cgccaagccc ggcgtcacgc tcgaggacgc
     1921 catcgagaac atcgacatcg gcggcccggc gatggtccgc tcctcggcga agaattacgc
     1981 gggcgtcgcc atcgtcaccg acccggccga ctacgccgac ctgcttgccg agatgaaggc
     2041 caacgacggc gcgctcgaac tcgtcacccg ctttaatttg gcgaagaagg ccttcaccca
     2101 cacggcgcgc tacgacggca tgatcgccaa ctggctgacc ggtctggccg acggcgccga
     2161 agccaagccc gacgccgcgg cgccggtacc gagcgtcttc cccgaccggc tgcagctcgc
     2221 cttcgaccgc gtcgagacgc tgcgctatgg cgagaactcg caccaaagcg ccgccttcta
     2281 tcgcgatacc aattccgtac aaggcagcat cgccgcctac caccaactcc agggcaagga
     2341 actcagctac aacaacatcg ccgatgccga cgcggcttgg gagtgcgtca agagcttcga
     2401 cgcgccctgc tgcgtcatca tcaagcacgc caatccctgc ggcgtcgcca tcgacggcac
     2461 gctgctcgcc gcctacgaga aggccttcaa gaccgactcg acctcggcct tcggcggcat
     2521 catcgccttc aacggcgaag tcggcatcga cgtcgtcgag gcgatgagtg cacgtaagca
     2581 cttcgtcgaa gtgctgatcg ccccggcgtt cacagccgaa gccaagacgg cgctggccgg
     2641 caaaaccaac ctgcgggtgc tcgaagtgcc gctgtcgaag acgcttaacg cgctcgagtt
     2701 caagcgcgtc ggcggcggcc tgctggtgca gacacccgat gccttcaccg cgcaggccga
     2761 tggcctgaag atcgtcacca aaaagcagcc gacccgggag cagatcgaag acctgctctt
     2821 cgccgagcgc gtcgccaagt tcgtcaagtc gaacgccatc gtcttctgcg gcggcggcat
     2881 gacactcggg gtcggcgccg ggcagatgtc gcgcgtcgat tcgaccaaga tcgccagcat
     2941 caaggcgcag cacgccggtc tagagctgaa aggctcggtg gtcgcgtctg atgccttctt
     3001 cccgttccgg gacggcgtcg acgtgctcgc tgcggccggc gccgtcgccg tcatccaacc
     3061 gggtggttcg atgcgcgacg aggaagtcat tgccgcggcc gacgaacacg gcctagcgat
     3121 ggtattcacg ggcgcccggc acttccggca ttgacactcg tgccgctggc accgacctct
     3181 gtgccgttgg cgcaaatccc cttgccggcg gaccggtgcc gaatcgaatc gcagtcgttc
     3241 gggccaatca tggcgctctg tgcggcgctg tcggtggcgc cgactgccgc cgccgagggc
     3301 gatggctggg ccacgcgcac tgccgaccac ctgtcgggca tcgccctcga cggcgacacc
     3361 gaagtctttc tgcccttcct cacctatcac ctgcgctacg cctacacccc ggaaaaaatc
     3421 gccagctacg aggaatcgcc gctcggcttc ggcatcggcc gtggccgctt cctcgacgac
     3481 ggcaactggg ccggcctctt cgtcatgggc tttcaggact cgcactcgcg gccgcagtgg
     3541 ctcgccggct acggctggca gaagatctgg aacacgggtc cgggcatccg catcgggctc
     3601 ggctacaccg ccttcctgac cgcccgcacc gacatcaacc actaccaacc aacgccggcg
     3661 atcctgcctc ttgcctcgat cggttacggc aatctgagca tcgaaggcac cttcgtcccc
     3721 ggcggaaccg gctacggcaa catcctcttt ttctgggcga aatggcgccc tgaccataag
     3781 gaatctccat gaaactcctc gtcattggcg gcggtggccg tgaacacgca ctggcctgga
     3841 aactggcgca atcgaccggt ctgcagaaag tctatgtggc acccggcaac gccggcaccg
     3901 cgcgcgagca tgagctcgaa aacgtcaaca tcaccgaccc gatcgcgctg gccgacttcg
     3961 ccgagcgcga gaaggttcac ctcaccgtcg tcggccccga ggcgccgctg gccgccggcg
     4021 tggtcaacat ctttcgtgcc cgcggcctca aggtcttcgg ccccacccgc gaagccgcac
     4081 agctcgaatc ctcgaaggat ttcgccaagc gattcatggc ccgccatcag atccccaccg
     4141 ccgaattcgc caccttcagc gagccgaagg ccgcgcatga atacgtcgat tccaagggcg
     4201 cgcccatcgt catcaaggct gacggcctcg ctgccggcaa gggcgtcgtc gtcgcgatga
     4261 ccgccgccga ggcacatcag gcgatcgacg acatgctttc cggcaacact ctgggcgatg
     4321 ccggcgcgcg cgtcgtcatc gaggaattcc tgagcggcga ggaagccagc ttcatcgtca
     4381 tggtcgatgg caagcacgtg cttccgctcg cctcctcgca ggaccacaag cgactcaagg
     4441 acggcgacct gggccccaac accggcggca tgggtgccta ctcgccggcg ccggtggtca
     4501 cgccggcgat ccacgcccgg gtcatgcgcg aagtgatcat gccgacggtc aagggcatgg
     4561 ccgccgacgg catacccttc accggctttc tctacgccgg cctgatgatc tcgcccgacg
     4621 ggacgatcaa aacactcgaa ttcaactgcc gcatgggcga ccccgaaacg cagccgatca
     4681 tgatgcggct gaagagcgac ctgcggacgc tctgcgagca tgccgtcgcc ggcacgctcg
     4741 acaaggtcga ggccgagtgg gaccgccgcg tcgcactcgg cgtggtgctc gccgcgcaca
     4801 actatccggc aacgccgcgc aagggagacg ccatcgacgg gctcgcgcag cagcacgacg
     4861 atgcgcacat cttccacgcc ggcaccgccg agcagagcgg ccaggtggtg acgtccggcg
     4921 gccgcgtgct ctgcgtcacg gcactcggcg acagcgtcaa gcaggcgcag aagcgcgcct
     4981 acgaggtcct tgcaggcgtt catttcgacg gcatgcagta ccgcagtgac atcggctatc
     5041 gcgccgtcgc ccgttgagct tggaccggac ccgctccacc atctgacgcc gggatacaat
     5101 cttcctccac catggacgtc atcccgctct tcttcgtgct cggcgtcgcc gcccgcctga
     5161 tccgctccga tctcaaggtc cccggcgccg tctacgaact gctggcaatc tacctgctgc
     5221 tggcgatcgg cctcaagggc ggcgtcgaac tcgcccgcca ggacgtgctg tcgctgctgc
     5281 cggccgtcgg cctggtcctg ctgatgggca tcgccctgcc actgcttgcc tttccgctcc
     5341 tcaagctgcg cttcgcacga gccgatgcag cgagcatcgc cgcccactac ggttcggtct
     5401 cggtggtcac ctacgcggtt tgcgcgaccg cgctgaccac gaggcaactc gactacgagg
     5461 cctacttccc gattttcgtc gccatcctcg aagtgccggc gatcctgatc ggcgtgctgc
     5521 tggcgcgaat cggcaccggc gaagccggca agccgaccaa atggggcccg ctcgcgcacg
     5581 agatattcag cagcaagagc gtcgtgctgc tgatcggcgg catcgccatc ggctggatcg
     5641 gcggaccgac cagcatcgag cctctggtgc cgctcttcta cacactgttc aagggcgcgc
     5701 tgtgcctgtt cctgctcgag atgggcctgg tcgccgccag ccgccttgat gacctgcgcc
     5761 gcgccggcgc cttcctcgtc gccttcggca ttctctttcc gttggtcggc gcagcggtcg
     5821 gactggcctg tggctggctg ctcggcttca cgcccggcgg agtcgcgctg ctgatgacgc
     5881 tgggcgccag cgcttcctac atcgccgcgc cggcggcgat gcgcatcgcc gtacccgagg
     5941 ccaacccgac cctttcgatc ggcgccgcgc tgggaatcac cttccccttc aacatcatgg
     6001 tcggcatccc gctctacatc gcgattgctg aacggctgcg gagctagcgc atgcagaacc
     6061 atccgaaaaa gctcttggtt atcatcgccg aggcagcgct cgagaagctg ctcgtcgagg
     6121 acatccttcg cctcggcgcg cacggctaca ccgtcctcga tgcgcgcggc gacggcaccc
     6181 gcggcaagcg gcaggcgagt tgggagggcg acagctcgat ccgcgtcgag gtgatctgcg
     6241 acgaggctac cgccgaacgc atcgccgacc acgtcagcga gacctacttc accaactacg
     6301 cggtgacact ctacgtcgcc gacgtccgcg tgcagcgctc gagcaagttc tgagcacggc
     6361 tcgccgggcc gcacccgcga gcacttcgat gcgcgcccac cgcgacggcg cggaacgcgc
     6421 cgattccatc tgcgtaacgt agatcaccga ctccgtttcg ggcggcccct cgcatcgctc
     6481 ccgcctcaac ccacgaccaa acggagaacc tcatgcaagc catcgtccgc aagaccctcg
     6541 gcctcgccac ctcggccgcc gtcgccctca ccctctcgac cggcgccctc gcctccgaca
     6601 agactcgcta cgagcgcctc ggtggcaacc cggcgctcga agccgtggtt ggcgaactgt
     6661 gggccaccac ctcggctgac gcacgcatca accactactt cgccaagacc aagcccgaag
     6721 tctttgctgg cctgatggtt gatttcctct gtcaggcggc cggcggcccc tgccagtaca
     6781 agggcaagga catggccggc gcacacaccg gcatgaagcg ctccgaagcg gacttcaacg
     6841 cactcgccga aaacg
//
LOCUS       DATDAA010000131         9519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_44184,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000131 DATDAA010000000
VERSION     DATDAA010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44184"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VIS73_04180"
     CDS             <1..517
                     /locus_tag="VIS73_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5762386.1"
                     /translation="ALFKGMSVLDNIMTGRNMKMKSNLFWQALRNPFGFGPAEKEEIA
                     HRAVVEQIIDFLEIQPYRKVPVGRLPYGLQKRVDLGRALAMEPQVLLLDEPMAGMNVE
                     EKQDMCRFILDVNDQLGTTIVLIEHDMGVVMDISDRVVVLDYGQKIGDGVPDEVRNNK
                     AVIDAYLGVSH"
     gene            530..1423
                     /locus_tag="VIS73_04185"
     CDS             530..1423
                     /locus_tag="VIS73_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5762387.1"
                     /translation="MTFFLEVLIGGLLSGVMYSFVALGFVLIFKASGVFNFAQGAMVY
                     FAALTFVGITEMDVPFWAAIPLAMAAMVALAWLTERIVLRQLVNQPGITLFMATIGLT
                     FLIEGIAQAMWGAEVRGLDIGIVDEPIPWLMENYDIGVSKFDLVAAGIAAVLVTVLAL
                     FFQYTRVGRALRAVADDHQAALSIGIPLRNIWVVVWSISGFVALVAGLLWGARNGVQF
                     ALTFTALKALPVLILGGFTSIPGAIVGGLIIGSTEKLAEVFLGGYVGGGIDSWFPYVL
                     ALAFLLVRPEGLFGEKHIDRV"
     gene            1484..2551
                     /locus_tag="VIS73_04190"
     CDS             1484..2551
                     /locus_tag="VIS73_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5762388.1"
                     /translation="MIYREAGQFKTSYAADQAVLPIKQDRIAIVMLIAVAYVAVPIIA
                     DQYWLSAILIPFLIFALAALGLNILTGYAGQLSLGSAAFMAVGAFAAYNFMLRVEGMP
                     LLLAFALGGVCAALVGIAFGLPSLRIKGFYLAVATLAAQFFVVWLLTKVEWFSNYSSS
                     GVITTQEMNILGFAFDTPEKRYLLVLSIVILLAMAAKNMVRSTVGRTWMAVRDMDVAA
                     EVIGIRMMRTKLTAFAISSFYCGVAGALYAYAYLGTVEPEGFNLDLSFRILFMIIIGG
                     VGSILGSFLGSAFIVLLPILLDVLTQAFLVHFLPSSISSNLQLIVFGGLIVFFLIVEP
                     HGLARLWQIAKEKLRLWPFPH"
     gene            2660..3988
                     /locus_tag="VIS73_04195"
     CDS             2660..3988
                     /locus_tag="VIS73_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5762389.1"
                     /translation="MKARPIAIIVAGALSAILAQTAIAQAKEQFVGLPSYRVGAYAAG
                     GTGIYGGWIDYMTLVNERDGGVNGVKLSWEECETEYNNARGVECYERMKNKGATGNSM
                     FSPLSTGITYSVLEKVPADQIPLVTIGYGRTDATDGSVFPWVFPLLTNYWSQNTAKIK
                     YIGMKEGGMDKLKGKKLVNLYHDSAYGKETIPVLDKQAEKYGFSVVHIPVAHPGNEQQ
                     SQWLKIRQEKPDWVILRGWGVMNPTALKNAAKVGFPRTKMVGVWWSGAEEDTIPSGSA
                     AKGFVAAGFNVAGTNYPLIKDIQKHVYAKGKGNMEDKSRVGSVYYNRGVVHGIIMVEA
                     IRTAQEKYGKGKAMTGEQMRWALEHLSIDSKRLKALGAEGFMPDLKVSCSDHEGSGKV
                     KFQEWDGTKWKVITDWIDADKSIVRPMIEESAAKYAAEKGITPRDCSKES"
     gene            4060..4893
                     /locus_tag="VIS73_04200"
     CDS             4060..4893
                     /locus_tag="VIS73_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5762390.1"
                     /translation="MSTTSHHAGAEQRYLAVNNVEVIYDHVILVLKGVSLEVPKGGIV
                     ALLGANGAGKTTTLKAISNLLRAERGDVTKGNVEFKGKRVDQLNPSELVKRGVIQVME
                     GRHCFGHLTIEENLLTGAYTRSVSSAQVKQDLEMVYHYFPRLKERRSSQAGYTSGGEQ
                     QMCAVGRAMMARPEMILLDEPSMGLAPQIVEEIFGIVQNLNEKEKVSFLLAEQNTMVA
                     LRFADYGYILENGRVVMEGDADELRNNEDVKEFYLGLSSSGRKSFKDVKHYRRRKRWL
                     S"
     gene            5047..6279
                     /locus_tag="VIS73_04205"
     CDS             5047..6279
                     /locus_tag="VIS73_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEY5762391.1"
                     /translation="MSFYDPLETRAPEDRERYLLNQLPRLVAHAKEKTSYFFNLLAGV
                     NPHDVHSRAALAQLPVTRKADLSEQQKRHKPFGGLNATAIGGLRHVFSSPGPIYEPEG
                     RAPDPWRMARALHAAGFRAGDLIHNCFAYHFTPGAFMFEGGAAKLGCPVFPGGTGQTE
                     MQVQAMADLAPVGYVGTPSFLKIILERADELGVEVSSLKKALVSGEAFPAATRRFFAG
                     EGITGRQCYATADLGLIAYESEAEDGLILDEEVIVEIVRPGTGDPVAAGEVGEVLVTT
                     FQYDYPLLRFATGDLSAELPGRSPCGRTNMRIRGWLGRADQTTKVKGMFVHPEQIAEV
                     VRRHQPVKRARLVVDNPDGNDRMVLRCEAEATCKDLGESLAADLREVTKLRGAVELVA
                     PGSLPNDGKVIEDSRRYD"
     gene            complement(6332..6763)
                     /locus_tag="VIS73_04210"
     CDS             complement(6332..6763)
                     /locus_tag="VIS73_04210"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HEY5762392.1"
                     /translation="MPNTLKDALTIDPQYRVGIPQVDREHQRLFEIIAEVNAGLDHAD
                     DKARAAALRHAIGELIEYTRSHFASEEALMKNAGFPGLDIHQLLHSDLLKRVREMQMR
                     IEIGDETATLDLSRFLASWLTEHILTADLEFGRFVSARPPA"
     gene            6843..7742
                     /locus_tag="VIS73_04215"
     CDS             6843..7742
                     /locus_tag="VIS73_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY5762393.1"
                     /translation="MSTALLLFPDFALILLGLALRRYFALGDHFWSGVEKLVYFILFP
                     ALLVHAVSRTQFEYAVAGPLVGAALASMLGAMLLMTPLKPLFGLSSIRAASIFQCGYR
                     FNSYIGLAAAGLLFGTPGLATMGLIIGAAVPLANLTAVSMLARHGEAGILREVLRNPL
                     IWATALGFALNVGGIAIPKPVDMMLERLADASIALGLLTVGAALRLSGSAWMAGAAIW
                     VLVVKLAALPAVAWLIGCWLALPAAQMGVLLIFAALPSASSAYILTMRMGGDGAAVAW
                     LISASTLLSMLTMPLWVALRALA"
     gene            complement(7747..8193)
                     /locus_tag="VIS73_04220"
     CDS             complement(7747..8193)
                     /locus_tag="VIS73_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HEY5762394.1"
                     /translation="MAGTQNDNDPMNFVRNLWGNVGFNLPGMVTPTMDTDELAKQISD
                     LKAVEGWLRMNLSMLQMTIQNLEMQRTTLTTVQALGRVAQDANESGATSDAAASLSQA
                     AMWPWAMMQQVQEQVAKQAEEAEKSATAAAVKSAGGEKAAKRSKKD"
     gene            8326..8973
                     /locus_tag="VIS73_04225"
     CDS             8326..8973
                     /locus_tag="VIS73_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5762395.1"
                     /translation="MLNILVVEDHALVREGLTRILRQVSDEEVTLHEAGNVDEALAIL
                     DSQADVDLVTLDLALPGVDGLTWLAAQRKRFPTVPFVVVSAYDDEATVRRVMRAGASG
                     FVSKSYSADDMLRALRKVLAGEVVEPFSAPTVLLADPPVPPAVTARAKDLGLSPRQAE
                     VLSLMAKGRSNRDIAQLLGLTEGTVKIHVTAIFRALGVASRTQALVALARLKIKI"
     gene            complement(8963..9346)
                     /locus_tag="VIS73_04230"
     CDS             complement(8963..9346)
                     /locus_tag="VIS73_04230"
                     /inference="COORDINATES: protein motif:HMM:NF022777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3135 domain-containing protein"
                     /protein_id="HEY5762396.1"
                     /translation="MSASPLPRFEEMARLAQENPADFERLRATLVEDFIAHSPRRLEQ
                     RLRGLQFRIDSSRRLAKSDYGAALRVFAMMWESFESLASLWRGLPDEMQNGVKPLPRS
                     AKVLRLGEPKPAAGADPAPDKPIRS"
BASE COUNT         1593 a         2979 c         3080 g         1867 t
ORIGIN      
        1 ggccctgttc aagggcatga gcgtgctgga caacatcatg accggccgca acatgaagat
       61 gaagagcaac ctgttctggc aggccctgcg caaccccttc ggcttcgggc cggcggagaa
      121 ggaggagatc gcgcaccgcg ccgtggtcga gcagatcatc gacttcctcg agatccagcc
      181 ctaccgcaag gtgccggtcg gccgcctgcc ctacgggctg caaaagcgcg tcgacctggg
      241 ccgcgcgctg gcgatggagc cgcaggtgct gctgctcgac gagccgatgg ccggcatgaa
      301 cgtcgaggag aaacaggaca tgtgccgctt catcctcgac gtgaacgacc agctcggcac
      361 gacgatcgtc ctgatcgagc acgacatggg cgtcgtcatg gacatttccg atcgcgtcgt
      421 cgtcctcgac tacggccaga agatcggcga cggcgtgccc gacgaggtgc gcaacaacaa
      481 agccgtgatc gacgcctatc tcggcgtctc gcactgacgg agtaccgcga tgacattctt
      541 tctcgaagtg ctgatcggcg gcctcctttc cggggtcatg tattccttcg tagccctcgg
      601 cttcgtgctg atcttcaagg cctcaggcgt cttcaacttc gcgcaggggg cgatggtcta
      661 ttttgcggcg ctgaccttcg tcggcattac cgaaatggac gtgcccttct gggccgcgat
      721 cccgttggca atggcagcga tggtggcgct ggcctggctg accgagcgaa tcgtcctccg
      781 ccagttggtg aaccagccgg gcattacgct gttcatggcc accatcggcc tgaccttcct
      841 tatcgaaggc atcgcccagg cgatgtgggg cgccgaagtc cgcgggctcg acatcggcat
      901 cgtcgacgaa ccgattccct ggctgatgga gaactacgac atcggcgtct ccaagttcga
      961 cttggttgca gccggcatcg ccgccgtgct ggtgaccgtg ctcgccctgt tcttccagta
     1021 cacccgcgtc ggccgcgcgc tgcgggcggt cgccgacgac catcaggcag ccctgtcgat
     1081 cggcattccg ctgcgcaaca tctgggtcgt cgtctggtcg atctcgggtt tcgtcgcgct
     1141 ggtggctggt ctgctctggg gtgcccgcaa tggcgtccag ttcgcgctga ccttcaccgc
     1201 gctgaaggcg ctgccggtgc tgatcctcgg cggctttacc tcgatcccgg gcgcgatcgt
     1261 cggtgggctg atcatcggtt cgaccgagaa gctcgctgaa gtcttcctcg gtggttacgt
     1321 cggcggcggc atcgattcct ggttccccta cgttctggcg ctggccttcc tgctggtgcg
     1381 accagagggc ctgttcgggg agaagcacat tgaccgcgtc tgaccgcgct gccgtaggcg
     1441 tgcgctcgca gcaacccacc agaacgattc gccggagcga cagatgattt atcgcgaagc
     1501 aggacagttc aagacgagct acgcggctga ccaggcggtc ctgccgatca agcaggatcg
     1561 tattgcgatc gtcatgctga ttgccgtcgc gtatgtcgcc gtaccgatca tcgccgacca
     1621 atactggctg tcggcgatcc tgatcccctt tctgatcttc gcgctggcgg cgctgggcct
     1681 aaatatcctc accggctacg caggccagct ctcgctcggc tcggcagcgt tcatggcggt
     1741 cggcgccttt gcggcctaca acttcatgct gcgcgtcgag ggtatgccat tgctgctcgc
     1801 cttcgcgctc ggcggcgtct gcgcagcgct ggtcggtatc gccttcggcc tgcccagctt
     1861 gcgtatcaag ggcttctatc tcgcggtggc gacgctcgcc gcacagttct tcgtcgtctg
     1921 gttgctgacc aaggtcgagt ggttctctaa ctactcgagt tctggcgtga tcacgaccca
     1981 ggagatgaac attctcggtt tcgccttcga tacgccggag aagcgctatc tgctggtact
     2041 gagcatcgtc attctcttgg cgatggcggc aaagaacatg gtgcgaagca cggtcgggcg
     2101 cacctggatg gccgtgcgcg acatggacgt cgccgccgaa gtcatcggca ttcgcatgat
     2161 gcggacaaaa ctcacggcgt ttgcgatcag ctcgttctac tgcggcgttg ccggtgcgct
     2221 ctacgcctac gcctacctcg gcaccgtcga acccgaaggc ttcaacctcg acttgtcgtt
     2281 ccgcatcctg ttcatgatca tcatcggtgg cgtcggctcg atcctcggct cctttctcgg
     2341 ctcggccttc atcgtgctct tgccgatcct gctcgatgtg ctgacgcagg cctttcttgt
     2401 ccatttcctg ccgtcctcga tcagctcgaa cctgcagctg atcgtatttg gcgggttgat
     2461 cgtctttttc ctgatcgttg agccgcacgg cctagcccgc ctgtggcaaa tcgcgaagga
     2521 gaaactcagg ctttggcctt tcccgcactg acaccgttcg cgcgggtgaa gttgaaggat
     2581 ccgatcttga cccggcggcc tgccgggccg gttggcagat gaaaacggtt caaatcgttt
     2641 tttcgaagga ggagacaaca tgaaagcaag accaattgcc atcatcgtcg cgggcgcgct
     2701 gtccgcaatc ctggcccaga ccgcgatcgc gcaggctaag gagcagttcg tcggtctgcc
     2761 aagctaccgc gtcggcgcct acgccgccgg tggcaccggc atctacggcg gttggattga
     2821 ctatatgacg ctggtcaacg aacgggatgg cggcgtcaac ggcgtcaagc tctcctggga
     2881 agagtgcgag accgagtaca acaacgcgcg cggcgtcgag tgctacgagc gcatgaagaa
     2941 caagggggcg accggaaatt cgatgttctc gccgctgtcg accggcatca cctactccgt
     3001 gctagagaag gtgcccgccg accagatccc gctagtgacg atcggctacg gccgcaccga
     3061 cgcaaccgac ggcagcgttt tcccctgggt cttcccgctg ctgaccaact actggtcgca
     3121 aaacaccgcc aagatcaagt acatcggcat gaaggaaggc gggatggaca agctcaaggg
     3181 caagaagctc gtgaacctgt atcacgattc ggcctacggc aaggaaacga tccccgttct
     3241 ggacaagcag gccgagaagt acggcttcag cgtcgtgcac attccggtcg cacacccggg
     3301 caacgagcag cagtcgcagt ggctgaagat ccgtcaggaa aagcctgact gggtgatcct
     3361 gcgcggctgg ggcgtgatga acccgacggc cctgaagaat gcggcaaagg tgggtttccc
     3421 gcgcaccaag atggtcggcg tgtggtggtc cggtgctgag gaggacacga ttccgtcggg
     3481 ttcggcagcc aagggcttcg tcgcggcagg gttcaacgtc gcgggcacga actatccgct
     3541 gatcaaggac atccagaagc acgtctacgc caagggcaag ggcaacatgg aagacaagtc
     3601 ccgggtcggc tcggtctatt acaaccgggg tgtcgtccac ggcatcatca tggtcgaggc
     3661 gatccgtacc gctcaggaaa agtacggcaa gggcaaggcg atgaccggcg agcagatgcg
     3721 ttgggcactc gagcacctgt cgatcgacag caagcggctc aaggcgctcg gtgccgaggg
     3781 cttcatgcct gacctcaagg tctcctgcag cgaccatgag ggctctggca aggtcaagtt
     3841 ccaggagtgg gacggcacca agtggaaggt catcaccgac tggattgacg ccgacaagtc
     3901 gatcgtccgg ccgatgatcg aggaatcggc ggcgaagtac gccgcggaga agggcatcac
     3961 cccgcgcgat tgctcgaagg agagctgatc gcccggtcag ggcctgccgg cccggggtga
     4021 ttcccgggcc ggtccttccc agcatctgcg gagtctttca tgtcgaccac aagccaccac
     4081 gcgggcgccg agcagcgtta cctcgccgtc aacaacgtcg aggtcatcta cgaccacgtg
     4141 atcctcgtcc tcaagggtgt ctcgctcgaa gtccccaagg gcggcatcgt cgccctgctc
     4201 ggtgccaacg gcgccggcaa gacgaccacg ctcaaggcga tctcgaacct gctgcgtgcc
     4261 gaacgcggcg atgtcaccaa gggtaacgtc gagttcaagg gcaagcgcgt cgaccagctc
     4321 aacccatctg aactggtcaa gcgcggcgtc atccaggtca tggagggacg gcactgcttt
     4381 ggccacctga cgatcgagga gaacctcttg accggggcct acacgcgttc ggtgtcatcg
     4441 gctcaggtca agcaggacct ggaaatggtt tatcactact tcccgcgcct gaaggagcga
     4501 cgctcctcgc aggctggcta tacctcgggc ggcgagcagc agatgtgcgc agtgggccgg
     4561 gccatgatgg cgcggcccga gatgatcctg ctcgacgagc cgtcgatggg tctggcgccg
     4621 cagatcgtcg aagagatctt cggcatcgtg cagaacctta acgagaagga aaaggtgagc
     4681 tttctgctcg ccgagcagaa caccatggtc gcgctgcgtt tcgccgacta cggctacatc
     4741 ctcgagaacg gtcgcgtggt gatggagggt gacgccgacg agttgcgcaa caatgaggac
     4801 gtcaaggagt tctacctcgg cctgtcgtcg tcggggcgca agagcttcaa agacgtgaag
     4861 cattaccgcc gccgcaaacg ctggctgtcg taggtcgcag tgcgcgccgc cgtgcttcgt
     4921 tgctcggcgg cttgcgtgcg gattgcacgc ggcgccacct tgcgcctcgc ctaccggcac
     4981 tactttgcga cctacgctca gcgcagctga gctgctttga tccgttacgg accgaggtga
     5041 atcgagatga gcttctacga cccgcttgaa acccgtgctc cggaggaccg ggagcgctat
     5101 ttgctgaacc agttgccgcg gctggtggcg catgccaagg agaagacctc gtacttcttc
     5161 aacctgctcg cgggggtcaa tccacacgac gtgcattcgc gcgcagcgct ggcgcagttg
     5221 ccggtgacgc gcaaggccga tctcagcgaa cagcagaagc gccacaaacc cttcggcggc
     5281 ctcaacgcga cggcgatcgg cggtttgcgc catgtcttca gttccccggg gccgatctac
     5341 gaacccgagg gccgcgcgcc cgatccctgg cggatggcgc gggcgctgca tgcggcgggc
     5401 tttcgcgccg gtgacctgat ccacaactgc tttgcctacc atttcacgcc gggcgccttc
     5461 atgtttgaag gtggcgctgc caagctcggt tgccctgtct ttccgggcgg caccgggcag
     5521 accgaaatgc aggtgcaggc gatggccgac ctggcgccgg tcggttacgt cggcacgccg
     5581 tcctttctca agattatcct cgagcgtgcc gacgagttgg gcgtcgaggt cagttcactg
     5641 aagaaggcgc tggtctccgg tgaggccttt cccgcagcca cgcgccgttt tttcgccggc
     5701 gaggggatca ccggccgcca gtgctacgcg acggccgacc tcggcctgat cgcctacgag
     5761 agcgaagctg aggacgggct gattctcgac gaggaggtca tcgtcgagat cgtccgcccg
     5821 ggtaccggcg accctgtcgc cgcaggcgag gtcggcgagg tgctggtcac caccttccaa
     5881 tacgactacc cgctgctgcg ctttgccacc ggcgacttgt cggcagagtt gccggggcgt
     5941 tccccatgcg gacggaccaa catgcggatc aggggctggc tcgggcgcgc cgaccagacg
     6001 accaaggtca aggggatgtt cgtgcatccg gagcagatcg ccgaagtcgt ccgccgccat
     6061 cagcctgtca agcgtgcccg cctggtcgtc gacaaccccg atggcaacga ccgcatggta
     6121 ctgcgctgcg aggcggaggc gacgtgcaag gatctgggcg aatcgcttgc cgccgatctg
     6181 cgcgaagtca ccaagctcag aggtgcggtt gaactggttg cgccgggaag cctgcccaac
     6241 gacggcaagg tgatcgagga ttcgcgacgt tacgactgag gcgcgcgcct cggtcggcga
     6301 gccggagcgc tgttccgcgc gactgccctg ctcaggctgg tgggcgagcc gatacgaagc
     6361 ggccgaattc gaggtcggca gtgaggatgt gctcggtcag ccacgacgcg agaaagcgcg
     6421 agaggtcgag cgtcgccgtt tcatcgccga tctcgattcg catctgcatc tcgcgaaccc
     6481 gtttaagcaa atcactgtgg agcagctgat ggatgtcgag cccggggaag ccggcgttct
     6541 tcatcagtgc ttcctcgctg gcgaaatgcg agcgcgtgta ttcgatgagt tcgccaattg
     6601 cgtggcgcag ggccgcagca cgcgccttgt cgtcggcgtg atctagcccg gcgttgacct
     6661 cggcaatgat ctcgaacagg cgttgatgtt cgcgatccac ctgcgggatt ccgacgcggt
     6721 attgcgggtc tatggtcagc gcgtccttga gcgtgttcgg cattccgtgc gtcccctgtt
     6781 gcagccggcc gattctgcca cagcgccgcc gggccgcggc ttctccgcgt agaatcgccg
     6841 acgtgtcgac ggccctgctg cttttcccgg attttgcgct gattctgctc ggcctcgcgc
     6901 tgcggcgcta cttcgcgctc ggcgatcact tctggagcgg cgtcgagaag ctcgtctact
     6961 tcatcctctt tccggcgctg ctggtgcacg cggtcagccg gacccagttc gaatacgcgg
     7021 tcgccggacc gctggtcggc gcggcgttgg cgtcgatgct cggcgcgatg ctgctgatga
     7081 caccgctcaa gcccctgttc ggactgtcgt cgatacgtgc cgcatcgatc ttccagtgcg
     7141 gctaccgttt caattcctac atcggactgg ccgcggcggg cctgctcttc ggcacgccgg
     7201 gtctggcgac gatgggcctg atcatcggtg ccgctgtgcc gctcgccaat ctcaccgcgg
     7261 tgagcatgct ggcgcggcac ggcgaggccg gcattctccg cgaggtgctg cgcaatccgc
     7321 tgatctgggc cacggcgctg ggcttcgcac tcaacgtcgg cggtatcgcg attcccaaac
     7381 ccgtggacat gatgctcgag cggcttgccg atgcctcgat cgccctcggg ctgctgaccg
     7441 tcggcgctgc gttgcgcctc tccgggagcg cctggatggc cggcgcggca atctgggtac
     7501 tggtggtgaa gctcgccgcg ctgccagccg tggcctggct gatcggctgc tggctggcgc
     7561 taccggcggc gcagatgggc gtgttgctga ttttcgcggc gctgccgagc gcgtcgtcgg
     7621 cctacatcct gacgatgcgc atgggcggcg acggcgccgc cgtagcatgg ctgatctcgg
     7681 cgagcacgct gctgtccatg ctcaccatgc cactctgggt tgcgctgcgg gcgctggcct
     7741 gagggtctag tccttcttgc ttcgcttggc agctttctcg ccgcctgccg acttgactgc
     7801 ggcggcggtc gccgacttct cggcctcttc ggcctgcttg gccacctgct cctgcacctg
     7861 ctgcatcatc gcccacggcc acatcgccgc ctgcgacagc gatgccgcag cgtcgctggt
     7921 ggctcccgat tcgttggcat cctgcgcgac acggccgagc gcctgcacgg tcgtcagcgt
     7981 cgtccgctgc atctcgaggt tctggatcgt catctgcagc atcgacaggt tcatccgcag
     8041 ccagccctcg acagccttaa ggtcgctgat ctgtttggcg agttcgtcgg tatccatcgt
     8101 cggcgtgacc atccccggga ggttgaatcc gacattgccc cagaggtttc tcacgaagtt
     8161 catcggatcg ttgtcattct gtgtgccggc catcttgatt ctccgtcttg ccttgcgtga
     8221 tcgagggttt cattatggca ccgcgatgcg acgtgcggag tcattgcagg atttggaagg
     8281 aatgattaca ttggcatcgt tcagggagtt cagctgggga aatcgatgtt gaacatatta
     8341 gtcgttgaag atcacgcgct ggtgcgcgag gggctgacgc gcatcctgcg gcaggtgagc
     8401 gatgaagaag tcacgctgca cgaagcgggc aatgtcgacg aggcgctagc gatcctcgat
     8461 tcgcaggccg atgtcgatct ggttacgctc gatctcgcgc tgcccggcgt tgacggcctc
     8521 acctggctgg ccgctcagcg caagcgtttc ccgacggttc ccttcgttgt cgtctccgcc
     8581 tatgacgacg aggccaccgt gcgccgggtg atgcgcgccg gcgcctcggg cttcgtctcg
     8641 aaaagctatt cggccgacga catgctgagg gcactgcgca aggttctcgc tggtgaagtc
     8701 gtcgagccct tctccgcgcc gacggtactg cttgccgacc cgccggttcc accggcggtg
     8761 acggcgcggg caaaggatct aggactgtca ccgcggcagg ccgaagtcct gtcgctgatg
     8821 gccaagggcc ggtcgaatcg tgatatcgcc cagttgctcg gcttgaccga aggcaccgtg
     8881 aagatccacg tgaccgcgat ctttcgtgcg cttggagttg ccagccggac gcaggcgctg
     8941 gtggcgctgg cccggctgaa gatcaagatc tgatcggttt gtccggcgcc gggtcggcgc
     9001 ccgctgccgg cttcggttcg ccaagccgga ggactttcgc cgaccgaggc agcggtttga
     9061 cgccgttctg catttcgtct ggcaagccgc gccagagcga ggccagcgat tcgaaactct
     9121 cccacatcat ggcgaagacg cgcagcgccg cgccgtagtc ggactttgcc agtcggcggc
     9181 tgctgtcgat gcggaactgc aggccacgca ggcgttgctc gagtcggcgc ggtgaatggg
     9241 cgatgaaatc ctcgaccagc gtggcgcgca ggcgctcgaa atcggcgggg ttttcctgcg
     9301 ccagacgggc catttcctcg aagcggggca gcggcgaggc actcatttcg gcctccttcc
     9361 gaattctgca gggaatgtag ttggggcagc gctcagggcg ccgagttccc gccggtcgga
     9421 aggtcgtagc gatagaggag gtagccgtcg gcgtcgatgg tctcactggg ctcggcgtcg
     9481 gggaccggga aactgttgct gaccagacag gcgccggcg
//
LOCUS       DATDAA010000132         5096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_289310,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000132 DATDAA010000000
VERSION     DATDAA010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289310"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VIS73_04235"
     CDS             <1..469
                     /locus_tag="VIS73_04235"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY5762397.1"
                     /translation="IDGRLRPIRYRTWRDDKQKEASAAFDWATLQVTQAAGSEPLAAG
                     TLDPVTVFYQYGIAGAAALPAEVRVANGRRIKLLKISLLGREKLRLPSGESVATRRLR
                     AIEDDGETTELWLAERYAGYPLQISHTDRDGASYRQIATSIRLEPHALAQPPR"
     gene            444..1706
                     /locus_tag="VIS73_04240"
     CDS             444..1706
                     /locus_tag="VIS73_04240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEY5762398.1"
                     /translation="MRWHNRLVEHAVVALARVGHFEHAADGVLSRYFRENPEIGHGDR
                     GAIAESVYAILRHWRTLTAAIGGEGERLPRRMFLAWLVRHEGANLREIEKLLRAGERD
                     WLATVKGFDAATLSPAERSELPDWLYERLLASNGEAATRELAESLKRPAPLDLRVNSL
                     KAEREAVRTALAETGIEAEPTPHSPLGLRVAGKPALAKNPLFVDGQIEVQDEGSQLLG
                     FLLGPKRGEMVVDFCAGAGGKTMLLGALMRSTGRLYAFDVSDKRLVKLKPRLARSGLS
                     NVHAVRIDNENDLRIKRLAGKIDRVLVDAPCSGLGTIRRNPDLKLRQDEESVARLAVT
                     QAAILAAAARLLKPGGRIVYATCSLLEAENDAIVDGFLADHPGFAALNAGEALAAAGI
                     DLPGERLRLSPVAGGTDGFFAALLERKT"
     gene            1703..2995
                     /locus_tag="VIS73_04245"
     CDS             1703..2995
                     /locus_tag="VIS73_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY5762399.1"
                     /translation="MNEDTEQSLGLLQRLWADLHEPEIVWQIGALLAALVIAWVVARV
                     ANRSALQAGGSDVARIGIGGLRRVVFPLVALALVYVARGLLSTLTDVTLFNVAVPLLL
                     SMAIVRVTVYILRHAFKSAKWLLASERWIAMIVWGGFALHLTGLSPIFIEMMQQVVLP
                     LGKDNVDLWTILRGAAMVLVTVLAALWLAGLIEARLMKAVTLDTSLRAVLVRLTKAFL
                     TLLAVLTGLAMVGIDITALSVFGGALGVGLGFGLQKIASNYVSGFIILLDRSIKLGNV
                     IAIDAQTTGLVTQITTRYTVVRTLGGIEYIIPNEHFVANIVQNQTHTDSNMRLAVQIS
                     VAYDSDLPRVLELCAQVAAAHPRVLNDPAPRAVIVAFADSGINLEVGFWIGDPEKGTG
                     GVRADISLDLWKAFREHAVSIPFPQREVRILGPAKEAS"
     gene            2992..3492
                     /locus_tag="VIS73_04250"
     CDS             2992..3492
                     /locus_tag="VIS73_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEY5762400.1"
                     /translation="MIPRITAPRFLALIGVAAVLLVVAGLVLGEVLRLKACPLCIFQR
                     LLYLLVGGFALAGAALYGSSIARRLLAGLAFLSALGGMATASYQTWLQAQPASPFECG
                     FGDPNLIERLVEWLGQLWPMMFLATGICSSKEWVFLGLSMAGWSVICFGALALALLPF
                     LRQRKG"
     gene            3508..4458
                     /locus_tag="VIS73_04255"
     CDS             3508..4458
                     /locus_tag="VIS73_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY5762401.1"
                     /translation="MRDFFAVLAFSFSVTGPIFVVLALGKFLRRRAVIDDRFVEVGSR
                     LVFNITLPALLFISVAKTRFSEAANLPMIAFGAGATLAAYLALEFAAKRAGIAPRDRG
                     VVVQGGFRSNMAIIGLAYVVNAYGDAGLVMASLYVGLVTILFNVLSVVTLSRSLDANE
                     GIGRILRGIVRNPLIIGIMLALPVSALGIPLPGILVRTGEYFAQMTLPLALLCTGASL
                     NFESLRTDTRSTAIAAGAKLVGVPLLFVLGGIALGFRGMELGILLLMAAAPSAAASYV
                     MVRAMGGNAPLAANIVALTTIGSILATSLGLTVLRAYALI"
     gene            4507..4788
                     /locus_tag="VIS73_04260"
     CDS             4507..4788
                     /locus_tag="VIS73_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762402.1"
                     /translation="MIRSHPDHWLVQTFEVACGMLFACAAMQSFQIAFFGLALALLMV
                     PILHHVRWESCAVQVMVLAPLTLVVIGMGVSGMALAGMQTVFFGFGFHF"
     gene            4906..>5096
                     /locus_tag="VIS73_04265"
     CDS             4906..>5096
                     /locus_tag="VIS73_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762403.1"
                     /translation="MDRRRFLAVMAAAAANGPVRAVAAAGAYETGLLWRIDKPGLRPS
                     YVFGTLHLPDARLLPLPEPV"
BASE COUNT          713 a         1708 c         1710 g          965 t
ORIGIN      
        1 catcgatgga cgcctgcgcc cgatccgcta ccggacctgg cgcgacgaca agcagaagga
       61 ggcgagcgcc gccttcgact gggcgacgct gcaggtgacg caggccgccg gttccgagcc
      121 gctggctgcc ggcacgctcg acccggtgac ggtcttctac cagtacggta ttgccggtgc
      181 agcggcgctg ccagccgaag tgcgcgtcgc caacggccgg cgcatcaagc tgctgaagat
      241 ttcgctgctc ggccgcgaaa aacttaggct gcccagcggt gaatcggtcg cgacgcgacg
      301 cctgcgcgcg atcgaggacg atggcgagac caccgaactg tggctggccg aacgctacgc
      361 tggctatccg ctgcagattt cccataccga ccgcgacggc gcaagctatc gccagatcgc
      421 cacttcgatt cgactggaac cccatgcgct ggcacaaccg cctcgttgaa cacgcggtcg
      481 tcgcgcttgc ccgcgtcggc catttcgagc acgccgccga tggtgtgctg tcgcgctact
      541 tccgcgagaa cccggagatc ggccacggcg accgcggcgc gattgccgag tcggtctacg
      601 ccatcctccg ccactggcgc accctgaccg cggccattgg cggcgagggc gagcgcctgc
      661 cgcggcgcat gttcctcgcc tggctggtgc gccacgaggg cgcgaacctg cgcgagatcg
      721 agaagctgct gcgcgccggc gagcgcgact ggctggcgac cgtcaaggga ttcgatgccg
      781 cgactctaag ccccgccgag cgcagcgagt tacccgactg gctctacgag cgactgctgg
      841 ccagcaacgg cgaggcggcg acgcgcgagc tggcggagtc gctcaagcgc ccggcgccgc
      901 tcgacctgcg tgtgaattcg ctaaaggccg agcgcgaggc ggtgcgtacg gcactggccg
      961 aaacgggcat tgaggccgag ccgacgccgc actcgccgct cggcctgcgc gtcgccggca
     1021 agccggcgct ggccaagaac cccttgttcg tcgacgggca gatcgaggtg caggacgagg
     1081 gcagccagct gctcggcttc ctgctcgggc cgaagcgcgg tgagatggtc gtcgatttct
     1141 gtgccggcgc cggcggcaag accatgctgc tcggcgcgct gatgcgttcg accggccgcc
     1201 tctacgcctt cgacgtgtcc gacaagcgac tggtcaagct caagccgcgg ctggcccgtt
     1261 cgggtctgtc gaacgtacac gccgtacgta tcgacaacga gaacgacctg cggatcaagc
     1321 ggctcgccgg caagatcgac cgcgtgctgg tcgacgcgcc gtgctcgggg ctcggcacga
     1381 ttcggcgcaa ccccgacctc aagctacgcc aggacgagga atcagtggcg cgtctcgccg
     1441 tgacgcaggc ggcgatcctc gccgctgccg cgcggctgct caagcccggc ggccggatcg
     1501 tctacgcgac ctgcagcctg ctcgaggccg agaacgacgc gatagtcgac ggcttcctcg
     1561 ccgatcatcc gggcttcgcc gcgctcaacg cgggagaagc gctggccgct gccggcatcg
     1621 acttgcccgg cgaacgcctg cggttgtcgc cggtcgccgg tgggaccgac ggatttttcg
     1681 cagcactgct ggaacggaag acatgaacga agacacggaa caatcgcttg gcctgctgca
     1741 acggctgtgg gccgacctgc acgaaccgga aatcgtatgg cagatcggtg cgctgctggc
     1801 ggctctggtc atcgcctggg tggtcgcccg agtcgccaac cgctcggcgt tgcaggcggg
     1861 cggcagcgat gttgcgcgaa tcggcatcgg cggtctgcgc cgagtggtgt ttccgctggt
     1921 ggcgctggcg ctggtctatg ttgcgcgggg cctgctgtcg acgctgaccg atgtgacgct
     1981 gtttaacgtc gcggtaccgc tcctgctgtc gatggcgatc gttcgcgtaa cggtctacat
     2041 ccttcgccac gctttcaaat cggcgaaatg gctgctcgcc tccgagcgct ggatcgcgat
     2101 gatcgtctgg ggtggcttcg cgttgcacct gaccgggctg tcgccgatct tcatcgagat
     2161 gatgcagcag gtggtgctgc cgctcggcaa ggacaacgtc gatctgtgga ccatcctgcg
     2221 cggtgccgcg atggtgctgg tgacggtgct cgcggcgctg tggctggccg ggctgatcga
     2281 ggcccggctg atgaaggcgg tcacgctcga taccagtctg cgggcggtgc tggtgcggct
     2341 gaccaaggcg ttcctgacgc tgcttgcggt gttgaccgga ctagcgatgg tcggcatcga
     2401 cattaccgcg ctctcggtgt tcggtggtgc gctcggtgtc ggcctcggct tcgggctgca
     2461 gaagatcgcc agcaactacg tgtcgggctt catcatcctg ctcgaccggt cgatcaagct
     2521 cggcaacgtc atcgccatcg acgcgcagac caccgggctg gtgacgcaga tcaccacacg
     2581 ctataccgtg gtgcgcacgc tcgggggcat cgaatacatc attccgaacg aacacttcgt
     2641 cgccaacatc gtgcagaacc agacccatac cgacagcaac atgcgcctcg ccgtgcagat
     2701 ctcggttgcc tacgattcgg acctgccgcg cgtactcgaa ctctgtgcac aggtcgccgc
     2761 ggcgcatccg cgcgtgctca atgacccggc tcctcgcgcg gtgatcgttg ccttcgccga
     2821 cagtggcatc aatctggaag tcggcttctg gatcggcgat cccgagaagg gcaccggtgg
     2881 tgtccgtgcc gatatcagcc tcgatctgtg gaaggccttc cgcgagcacg ccgtgtcgat
     2941 tccgtttccg cagcgcgaag tgcgcatact cggccctgcc aaggaggcgt catgattccc
     3001 cggattaccg ctccgcgttt tctggcgctg atcggcgtcg ccgcggtcct gctggtcgtc
     3061 gccggcttgg tgctcggcga agtgctgcgg ctcaaggcct gtccgctgtg catcttccag
     3121 cgcctgctct acctgctggt cggcggtttc gcgctggccg gcgcggcgct ctacgggagc
     3181 agcattgccc gccgcctgct cgccggtctg gccttcctct cggcgctggg cggcatggcg
     3241 acggcgagct accagacctg gctgcaggcg cagccggcgt cgcccttcga gtgcggtttc
     3301 ggcgatccta acctgatcga gcggttggtc gaatggctgg ggcagttgtg gccgatgatg
     3361 ttcctcgcca ccggcatctg ttcgagcaag gagtgggtct tcctcggcct gtcgatggcc
     3421 ggctggtcgg tgatttgctt cggcgcgctg gcgcttgcgc tgctgccctt ccttcgccag
     3481 cgcaagggct gaagcccgcg ggacggcgtg cgcgacttct tcgccgtcct ggcgttttcc
     3541 ttctcggtca ccgggccgat attcgtcgtc ctcgccctcg gcaagttcct gcgccgccgc
     3601 gcggtcatcg atgatcgttt cgtcgaggtt ggttcgcggc tggtcttcaa catcacgctg
     3661 ccagcgctgc tcttcatcag cgtcgccaag acgcgtttct ccgaagccgc caacctgccg
     3721 atgatcgcct ttggcgccgg tgcgacgctg gcggcttatc tggcgctgga gttcgccgcc
     3781 aaacgcgccg gcatagcgcc gcgcgaccgc ggcgtggtgg tgcagggcgg gttccgctcg
     3841 aacatggcga tcatcggcct cgcctacgtc gtcaacgcct acggcgacgc cggcctggtc
     3901 atggcctcgc tctacgtcgg cctggtaacc atcctcttca acgtgctctc ggtggtgaca
     3961 ctgagccgct cgctcgacgc caacgagggc atcggtcgca tcctccgcgg catcgtccgc
     4021 aatcccctga tcatcggcat catgctggcg cttccggtct cggcgctggg gattccgctg
     4081 ccggggattc tcgtgcgcac tggcgagtac ttcgcgcaga tgacgctgcc gctcgcgctc
     4141 ctgtgcaccg gcgcctcgct caacttcgag agcctgcgca ccgacacgcg gagcacggcg
     4201 attgccgccg gcgccaagct ggtcggcgtg ccgctgctgt tcgtgctcgg cggcatcgcc
     4261 ctcggctttc gcggcatgga actcggcatc ctgctgctta tggctgctgc gccatcggcc
     4321 gcggccagct acgtgatggt gcgggcgatg ggcggcaacg cgccgctggc cgccaacatc
     4381 gtcgcactga cgacgatcgg ctcgatcctc gcgaccagcc tgggcctcac ggtcctgcgt
     4441 gcctacgcgc tgatttgagg cggcagccgt gacggttgcc tcaggcttgc tcgggccaag
     4501 cttttcatga tccgcagcca cccagaccac tggctggtgc aaaccttcga ggtcgcctgc
     4561 ggaatgcttt tcgcctgcgc ggccatgcag tccttccaga ttgcgttctt cggcttggcg
     4621 ctcgcgctgc tgatggtccc catcctccat cacgtgcgct gggagagctg cgcggtgcag
     4681 gttatggtgt tggcgccgtt gacgctggtg gtgattggca tgggcgtcag cggtatggcg
     4741 cttgccggca tgcagacggt gttcttcggc ttcggctttc atttctgaac ggcgggaggc
     4801 cgacccggcg cgtgagtcgg ccagctccct gacgttccgg cgggttgaac cgccgtcact
     4861 gccgcctgtc tccacaggcg gcagcttttt tttcggaggt ctggggtgga ccgtcggcgc
     4921 tttctcgcgg tgatggcggc agctgcggca aacggtccgg tgcgtgccgt tgccgcagcc
     4981 ggcgcctacg agaccggcct gctttggcgg atcgataagc cgggtctgcg gccgagctac
     5041 gtcttcggga ccctgcatct gcccgacgcc cgcctgctgc ctttgcccga gcccgt
//
LOCUS       DATDAA010000133         3003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_148937,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000133 DATDAA010000000
VERSION     DATDAA010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148937"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1072
                     /locus_tag="VIS73_04270"
     CDS             158..1072
                     /locus_tag="VIS73_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY5762404.1"
                     /translation="MKVRIASTQYMLRPIKGWDDFERQVHLMLDAAGEYRPQFVLLPE
                     IFTTQLMSFLPRQSVDLAVRALDQFTERYKALMRAHAMRYGYYLIAGSHPTIRDGELY
                     NTAFLFSPHGDVYEQDKTHRTRWERERWKTSSGHRLHLWETEFGDISILVCYDVEFPE
                     LARMVCEAGADILFVPSSTDDRQGFWRVRYCRHARAIENQVYVAMSSTVGSLPVEGLG
                     LHYGQASILTPSDFPFSRDGIAAEGIANTEQIIIADVDLDLLFENRVNGTTIPLYDKR
                     DELYRSPVIVTKTGPGAATTARPVRITG"
     gene            complement(1062..>3003)
                     /locus_tag="VIS73_04275"
     CDS             complement(1062..>3003)
                     /locus_tag="VIS73_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEY5762405.1"
                     /translation="GSLLDKAGAGNYMMQVSFAMLGHLRGGPAKVAVVSSALNGLISG
                     SSVSNVVSGGIFTIPLMSKAGYGGIKAGAIETSSSVNGQIMPPVMGAAAFLMVEYVGI
                     PYTDVVKHAFLPALISYIALFYIVHLEALKLGLEPTARARERGLSQKLTAWGLGISGT
                     VVVCGAIYFVAAATKSLFGAAAPWILGLLLLGLYLYTVWVAARCPDLPDDVDVENPVL
                     PDTWPTVKAGIHFLIPIGVLIWCLMIEELSPALSAFWATVSLIVLMGTQRPLIALLRR
                     RGGADAGLRRGIVEIVEGLNDGARNMIGIAIATGTAGIIVGGITLTGLGFRMTDFVEV
                     VSQGNVVVMLLFTALVCLVLGLGVPTTANYILVATLMAPVIVELGAQAGLVIPLIAVH
                     LFVFYYGIMGDITPPVGLASFAAAAISGEDAIKTGIQGSLYALRTVVLPFVFVFNPTL
                     LLIDVESWPELMLVALAATAASLVFAAATLGWFRTRCRWWEVGLLLLATFALFRPEYF
                     MDKIAAPYRTAPAKDIVHVAQSLGENQRLVMVIAGMTLEGEDVRKTVAVRLAEPGEGR
                     KRLAEAGLTVMVLGDDVRVAAVRFGSRARKSGFEQGWQIDSLKVPADRPSQHWIYLPA
                     LLLVALVFYTQGRRLRRPAPSP"
BASE COUNT          594 a         1011 c          931 g          467 t
ORIGIN      
        1 tgcttttcca gctgaaggaa gaattccagt attgcggcgt catccacggc tacctgccgt
       61 cggacgagga gtcacgcggc aacgccacga tcatcgtctg gcgcaatgac cgctacaaac
      121 cgacgcagcc gacggttatc ccggaaggac cgatcctgtg aaagttcgca tcgcctcgac
      181 ccagtacatg ctccgcccga tcaagggctg ggacgacttc gagcggcagg tccatctcat
      241 gctcgatgcc gccggcgaat accgcccgca gttcgtgctg ctgccggaga tattcaccac
      301 gcagctcatg tcgttcctgc cgcgccagtc ggtcgacctc gctgtgcgtg cgctcgatca
      361 gtttacggaa cgctacaagg ccttgatgcg cgcgcatgcg atgcgctatg gctactacct
      421 gatcgccggc agccatccga cgatccgcga cggcgagctg tataacaccg cgttcctctt
      481 ctcgccgcac ggcgatgtct acgaacagga caagacgcat cgcacgcgct gggaacgcga
      541 acgctggaag acgagctcgg gccaccgcct gcacctctgg gaaaccgagt tcggcgacat
      601 ctcgatcctg gtctgctacg acgtcgagtt tcccgagctc gcgcgcatgg tgtgcgaagc
      661 gggagcggac atcctgttcg tgccctcaag caccgatgat cgccagggct tctggcgcgt
      721 gcgctattgc cgccacgcac gtgccatcga gaaccaggta tacgtcgcga tgtcgagcac
      781 cgtcggctcg ctgcccgtcg aagggctcgg actgcattac ggtcaagcga gcattctcac
      841 gccgtccgac tttcccttct cgcgcgacgg tattgcggcc gaaggcatcg ccaacaccga
      901 acagatcatc atcgcggacg tcgatctcga cctgctgttt gagaaccgcg tcaacggcac
      961 gacgattccc ctctacgaca agcgcgacga gctttaccgg tctccggtca tcgtcaccaa
     1021 gacggggccc ggcgccgcaa ccacggcgcg gccggtgcgt attacgggct aggcgcgggg
     1081 cggcgcaggc gccggccctg cgtgtagaac accagcgcga ccagcaacag tgccggcagg
     1141 tagatccagt gctgtgaggg acggtcggcc gggaccttga gcgagtcgat ctgccagccc
     1201 tgctcgaagc ccgacttgcg cgcccggctg ccgaagcgca cagcggcgac gcgcacgtcg
     1261 tcaccgagca ccatgaccgt cagccctgcc tccgccaggc gcttgcggcc ctcgcccggt
     1321 tcggcgagac gcacggcgac ggtcttgcgc acatcttcac cctcgagcgt catgcccgcg
     1381 atcaccatca ccaatcgctg gttctcgccc agcgattgcg cgacatgcac gatgtccttg
     1441 gccggcgcgg tgcggtacgg tgctgcgatt ttgtccatga agtactccgg ccggaacagc
     1501 gcaaaggtcg cgagcagcaa caggccaact tcccaccacc ggcagcgcgt ccgaaaccag
     1561 cccagggttg ccgcggcaaa cacgagcgag gcagccgtgg cggcgagtgc gaccagcatc
     1621 agctccggcc agctctcgac atcgatcagc aacagggttg gattgaagac gaacacgaac
     1681 gggagtacga ccgtgcgaag tgcatagagc gagccttgaa tgccggtctt gatcgcgtct
     1741 tcgcccgaga tcgccgcggc ggcaaagctc gccaggccga cgggcggtgt gatgtcgccc
     1801 atgatcccgt agtaaaaaac gaacaggtgc accgcgatca gcgggatcac caatcccgcc
     1861 tgcgccccga gctcgacgat caccggcgcc atgagcgtcg ccaccaggat gtagttcgcg
     1921 gtggtcggca cgccgaggcc gagcacgagg cagaccagag cggtaaacag cagcatcacc
     1981 accacgttgc cctgcgaaac gacctcgacg aagtccgtca tgcggaaacc aagcccggtg
     2041 agcgtgatgc cgccgacgat gattcccgcc gtgccggtcg cgatcgcgat gccgatcatg
     2101 ttgcgcgcgc cgtcgttcag tccctcgacg atctcgacga tacccctgcg caatccggca
     2161 tccgcgccgc cgcgccggcg cagcagcgcg atgagcgggc gctgagtgcc catcaggacg
     2221 atgagcgaga ccgtcgccca gaaggcggac agtgcgggcg agagttcctc gatcatcagg
     2281 caccagatca gtaccccgat cggaatgagg aagtgaatcc cggccttcac ggtcggccag
     2341 gtatccggca ggaccggatt ctcgacgtcg acgtcgtcgg gcaggtcagg gcagcgtgcc
     2401 gcgacccaca ccgtgtagag atacaacccc agcaacagta gccccaggat ccagggcgct
     2461 gccgcgccga acaggctctt cgtcgcagcg gcgacgaaat agatggcgcc acagaccacg
     2521 accgtgcccg aaatgccgag cccccaggcg gtcagcttct ggctgagtcc gcgctcgcgc
     2581 gcccgcgccg tcggttccag gccgagcttc agcgcctcga ggtggacgat gtagaaaagc
     2641 gcgatgtagg aaatcagcgc cggcaggaat gcatgtttga cgacgtcggt gtaaggaata
     2701 ccgacgtact ccaccatcag aaaggcggcc gcgcccataa cgggcggcat gatctggccg
     2761 ttcacggacg acgaggtctc gatcgcgccc gccttgatgc cgccataccc cgcctttgac
     2821 atgagcggga tcgtgaagat gccacccgag acgacgttcg aaaccgacga gcccgagatg
     2881 aggccgttca gcgccgagga aacgacggcg accttggccg ggccgccgcg cagatggccg
     2941 agcatcgcaa aagacacctg catcatgtag ttgcccgcac ccgccttgtc gaggagcgag
     3001 ccg
//
LOCUS       DATDAA010000134         6138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_289466,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000134 DATDAA010000000
VERSION     DATDAA010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289466"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VIS73_04280"
     CDS             <1..216
                     /locus_tag="VIS73_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEY5762406.1"
                     /translation="NVFLETAASGTPAPNTPGSNGVGLLNQGYVETSNVNVAEELVAM
                     IQTQRAYELNSKAISTSDQMLARLSQL"
     gene            219..881
                     /locus_tag="VIS73_04285"
     CDS             219..881
                     /locus_tag="VIS73_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEY5762407.1"
                     /translation="MNALLLPALAAAVLVGCASAPPTAVQQPMSVRPQPQPEIVAAPG
                     SIFQAQTGRGLFDDRRASLVGDTLTINIVERNTASAASSSSADRSANAGASVGAISGL
                     PGRSLVGLGLDASSSNSFEGSGESAANNVFTGTITVTVVDVLANGNLIVSGEKQVAIN
                     QGNEFIRFSGVVNPATVTTANTVNSSQVADARIEYKGSGYINESQQMGWLARFFLNVL
                     PF"
     gene            912..2024
                     /locus_tag="VIS73_04290"
     CDS             912..2024
                     /locus_tag="VIS73_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEY5762408.1"
                     /translation="MLLRLNLLQALAVVLIALATPFAHAERIKDLANVQGVRSNQLVG
                     YGLVVGLDGSGDQTTQAPFTTQSLINMLSQLGIQLPPGQSLQLKNVAAVMVTASLPPF
                     ARNGQQLDVTVSSVANAKSLRGGTLLMTPLKGADGRIYAVAQGNLLVGGAGAGGGSTA
                     VQINHLSAGRISGGATVERAVETPVGQGEFVHLELRESDFSNVQRVAEAINASFGQPL
                     AVPVDGRQIRIRAPDDPGSRVAFLGRIENIDVSRNLAAAKVVVNPRTGSVVMNQTVTL
                     EPCAVAHGNLSVVVAKRTDVDQPPPLSGGQTVVSEQTDVSLTQEGGALMSVPAGANLA
                     DVVKALNALGAKPLDLLAILQAMRAAGALRAELEVI"
     gene            2040..3014
                     /gene="flgJ"
                     /locus_tag="VIS73_04295"
     CDS             2040..3014
                     /gene="flgJ"
                     /locus_tag="VIS73_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HEY5762409.1"
                     /translation="MLGHDLTSQLAADAKSLNDMRTLARTSPREGVRVAAQQFEAYFL
                     QMVMKSMRDATPSDGLFDSDQSRMYTGLLDQQLTQSLSSRGTVGFARMIEAQLAAQLE
                     RLAPPADGAAAGTGALPPGATVQPAVPTPTSNASPAQAAVQSAPARPAAHSAAAIDNP
                     VPATDWRAETPREFVERVWPHAVEAAAELKVPPHYLVAHAALESGWGQHEPLREDGSP
                     SFNLFGVKAGRGWAGDSVSARTDEYVDGQRVTANERFRAYDSYGESFRDYARLLAGSQ
                     RYAGVLGASDGMDFATNLQQAGYATDPMYADKLARIIGGNTLKHALLG"
     gene            3083..5248
                     /gene="flgK"
                     /locus_tag="VIS73_04300"
     CDS             3083..5248
                     /gene="flgK"
                     /locus_tag="VIS73_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEY5762410.1"
                     /translation="MSFLSVGVTAINAAQLGLFTTEHNIANAATPGYNRQQTAQTTNT
                     PTATGSGFIGQGTRVSTINRVYDQFIAAQVNQSQTRLSELETYYDEISRIDNMLGDPN
                     SGLSPALQDFFRGVQQLAANPATAPSRQALVSSAEALVSRVQGLGARLGELYESVDGQ
                     IGSTVDAINSFAQQIAELNQQIVIAEAAVQQPANDLLDQRDHLIAELNKLIRVSPITE
                     SDGSISVFIGTGQQLVVSTRANTLSAQPALADPTRITVGLRNFGTIQELPESLINGGE
                     LAGLLRFRNESLDNTANQLGRLTASFALTFNAQHDLGQDLLGASGAANIADFFEIGAP
                     SIVGNTLNGGNAAISGAFVAPPPMRLNNGAFSLTYDVTTAAYTVHRLDGSGLSFTDTD
                     LPTLLDTVETATGARLDLANGNFMTQVGTSDYRLTFLGGGNYSLRRLSDNLTFGPDTL
                     ANLSALIAPTEGFTLANPSGAPAVGDTFLIRPTRDIADNFTLNAAIVSDPRRIAAALP
                     VRSETGATNLGSGRITVESLVPGYVAPAAASPFTLTYDVATNAFTVGGLAAGTTVAVT
                     TPSATTVTTLPATVPYAADALYTVNGITFRLSGSPGQGDSFRLEANTAGIADARNALA
                     LGELQTQQTIAGGTTNFQSAYAQLVSSVGNKSREIRVTRDAQTALRDQAVASREAMSG
                     VNLDEEAANLLRYQQAYQAAARVMQVSGTLFEELLSIGR"
     gene            5270..>6138
                     /gene="flgL"
                     /locus_tag="VIS73_04305"
     CDS             5270..>6138
                     /gene="flgL"
                     /locus_tag="VIS73_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HEY5762411.1"
                     /translation="MRISTNQIYSAGTENINRNQSELLRLQNQISTGRRILRPEDDPV
                     AAAQALVATQSQQINRQHQENQGNARAQLGLLDGQLESLGNVIQDVRERTIQLGNAAL
                     TDRERGFIAEELESRLEQMLSIANAQHGEGLYLFAGFQGASVPFARAPGATGPFNAAN
                     PLVNFLGDQGQRSLQVDGSRQMPVNISGLDLFMNILNGNGTFTTSASATNGGTGIADA
                     GTVTNVALWNASTVQPQDFRVEFGVDTTVNPNVLKYNLIDNASGNSMFTNAAPGTPDT
                     AGWQVFTPGQAIV"
BASE COUNT         1095 a         2221 c         1888 g          934 t
ORIGIN      
        1 aacgtcttcc tcgaaaccgc cgccagcgga acgccggcgc cgaacacgcc gggcagcaat
       61 ggtgtcggcc ttctcaacca gggctacgtc gagacttcca acgtcaacgt cgccgaggaa
      121 ctggtagcga tgatccagac ccagcgcgcc tacgagctca actcgaaggc gatctcgacc
      181 tccgaccaga tgctcgcgcg tctgtcgcag ctctgatcat gaacgccctc ctgcttcccg
      241 ccctcgccgc cgctgtgctg gtcggctgcg cctcggcgcc gcccaccgcc gtccagcagc
      301 cgatgtcggt gcggccgcag ccgcaaccgg aaattgttgc cgcccccggc agcatcttcc
      361 aggcgcagac cgggcgcggc ctgttcgacg accgccgggc gagtctcgtc ggcgacacgc
      421 tgacgatcaa catcgtcgag cgcaataccg cctcggccgc ttcgagcagc tcggccgacc
      481 gcagcgccaa tgccggtgcc tcggtcggtg cgatcagcgg cctgcccggc cggtcgctgg
      541 tcggcctcgg gctcgatgca tcgagcagca acagcttcga gggatcgggc gaatcggccg
      601 ccaacaacgt gtttacgggc accatcacgg tgaccgtggt cgacgtgctg gctaacggta
      661 acctgatcgt ctccggcgaa aagcaggtcg cgattaacca gggcaacgag ttcatccgct
      721 tctccggcgt tgtcaatccg gcgaccgtga ccacggccaa cacggtcaat tcatcgcagg
      781 tcgccgacgc ccggatcgag tacaagggca gcggctacat caacgaatcg cagcagatgg
      841 gctggttggc acgtttcttc cttaacgtct tgccattctg acccggaacg ggcgccgcgc
      901 ccgatgacga catgctgctc cgcctgaatc tgctccaagc actcgcagtc gtactgatcg
      961 cactggcaac cccgttcgcc cacgccgagc ggatcaagga tctcgccaac gtgcagggcg
     1021 tgcgcagcaa ccagctggtc ggctacggcc tcgtcgtcgg cctcgacggc agcggcgacc
     1081 agacgacgca ggcgcccttc acgacgcaga gcctgatcaa catgctctcg cagctcggca
     1141 tccagctgcc gccggggcag tccctgcagc tgaagaacgt cgccgcggtg atggtcacgg
     1201 caagcctgcc gcccttcgcg cgcaacggcc agcaactcga cgtcacggtt tcctcggtcg
     1261 ccaacgccaa gagcctgcgt ggcggtacgc tcttgatgac gccgctcaag ggggccgacg
     1321 gccgcatcta cgccgtcgcc cagggcaacc tgctggtcgg tggcgccggt gccggcggtg
     1381 gtagcacggc ggtgcagatc aatcacctct ccgccggacg gatatccggc ggcgcgacgg
     1441 tcgagcgcgc cgtcgagacg ccggtggggc agggcgagtt tgtccacctt gaactgcgcg
     1501 agagcgactt tagcaacgtc cagcgcgtcg ctgaggcgat caacgcgagc ttcggccagc
     1561 cgctggcggt gcccgtcgac ggccggcaga tacgcatccg cgcccccgac gatccaggca
     1621 gccgcgtcgc cttcctcggg cgcatcgaaa acatcgacgt gtcgcgcaac ctcgccgcgg
     1681 ccaaggtggt cgtcaatccg cgcactggtt cggtggtgat gaaccagacg gtgacgctcg
     1741 aaccctgcgc cgtcgcccac ggcaacctct cggtggtcgt cgccaagcgc accgacgtcg
     1801 accagccgcc gcccttatcg ggcggccaga ccgtcgtcag cgagcagacc gacgtcagcc
     1861 tgacgcagga aggcggggcg ctgatgtcgg tgccggccgg cgccaatctg gccgacgtgg
     1921 tcaaggcgct caacgccctc ggcgccaaac cgctcgacct gctggcgatc ctccaggcaa
     1981 tgcgcgccgc cggcgcgctg cgtgccgaac tcgaagtgat ctgacggggg acgaaaccca
     2041 tgctcggtca cgacctcaca tcgcaactgg cagccgacgc caagtcgctc aatgacatgc
     2101 ggacgctggc gcggaccagc ccgcgcgagg gcgttcgggt cgccgcccag cagttcgagg
     2161 cctacttcct gcaaatggtg atgaagagca tgcgcgatgc gacaccgtcg gacgggctct
     2221 tcgacagcga ccagtcgcgg atgtacaccg gcctgctcga ccagcagctg acgcagagcc
     2281 tgtcgagccg cggcaccgtc ggtttcgcga gaatgatcga ggcgcagctc gccgcccagc
     2341 tcgagcgcct ggcgccaccg gccgatggcg cggccgcggg caccggcgcg ctgccgccgg
     2401 gggcgaccgt gcagccggca gtcccgaccc cgacgtcgaa cgcaagtccg gcgcaggccg
     2461 ccgtgcagtc ggcgccggca aggcctgccg cccattccgc agctgcaatc gacaaccccg
     2521 ttccggccac cgactggcgc gccgagacac cgcgcgagtt cgtcgagcgc gtctggccgc
     2581 acgctgtcga agccgcggcc gaactcaagg tgccgccaca ctacctggtc gcccacgccg
     2641 cgctcgagtc gggctggggc cagcacgaac cgctgcgtga agacggatcg ccctcgttca
     2701 atctgttcgg cgtcaaggcc gggcgcggct gggccggcga ttcggtcagc gcccgcaccg
     2761 atgaatacgt cgatggccag cgcgtcaccg ccaacgagcg tttccgcgcc tacgactcct
     2821 acggcgagtc cttccgcgac tacgcgcggc tgctcgccgg ctcgcagcgc tacgccggtg
     2881 tgcttggcgc gagcgatggc atggactttg cgacgaacct gcaacaggcg ggatacgcca
     2941 ccgatccgat gtatgccgac aagctggccc gcatcattgg cggcaacacg ctgaagcacg
     3001 cgctgctcgg ctgattcccg ttgcaactga aagcccgtct tgacgggcat gtgtcgcccg
     3061 cgggcgacga aggaggtggg aaatgtcatt tctgtcggtt ggagtgaccg cgatcaatgc
     3121 ggcgcaactt ggcctgttta cgaccgagca caacatcgcc aatgccgcga cgccgggcta
     3181 caaccgccag cagaccgcgc agacgaccaa tacacccacc gccaccggct cggggttcat
     3241 cggccagggc acccgcgttt cgacgattaa ccgcgtctac gaccagttca tcgccgccca
     3301 ggtcaaccag tcgcagacgc ggctctcgga gctcgagacc tactacgacg aaatctcgcg
     3361 catcgacaat atgctcggcg atcccaactc gggcctgtcg ccggccctgc aggatttttt
     3421 ccgcggcgtg cagcagctcg ccgctaaccc ggcaacggcg ccgtcgcggc aggcgttggt
     3481 ctcatcggcc gaggcgctgg tgtcgcgggt acagggactc ggtgcgcggc tcggcgaact
     3541 ctatgagtcg gtcgatgggc agatcggcag cacggtcgac gcaatcaact ccttcgccca
     3601 gcagatcgct gaactcaacc aacagatcgt catcgccgag gccgccgtgc agcagcccgc
     3661 caacgacctg cttgaccagc gcgaccacct gatcgccgaa ctcaacaagc tcatccgcgt
     3721 ctcgccgatc accgaatccg acggcagcat cagcgtcttc atcggcaccg gccagcaact
     3781 tgtcgtcagt acgcgtgcga acacgctgtc ggcgcagccg gccctggccg acccgacgcg
     3841 gatcaccgtc ggcctgcgca acttcggcac cattcaggaa ttgcccgagt cactgatcaa
     3901 cggcggcgag ctcgcgggcc tgcttcgctt ccgcaacgag tcgctcgata acaccgccaa
     3961 ccagctcggc cgtctcaccg cttccttcgc gctgaccttc aatgcgcagc acgatctcgg
     4021 gcaggacctg ctgggcgcct cgggcgcggc aaatattgcc gacttcttcg agatcggcgc
     4081 gccatcgatt gttggcaaca cgcttaacgg cggcaacgcg gcaatcagcg gcgccttcgt
     4141 cgcgccgcca ccgatgcggc tcaacaacgg agcgtttagc ctcacctacg acgtgaccac
     4201 cgccgcctac acggtccatc gcctcgacgg cagcggcctg tcgtttaccg acaccgacct
     4261 gccgaccctg ctcgatacag tcgaaaccgc gaccggtgcc cggctcgacc tcgccaacgg
     4321 caacttcatg acacaggtcg ggacttccga ctaccggctg accttcctcg gcggcggcaa
     4381 ctactcgttg cgccggctga gcgacaacct gacgttcggg cccgacaccc tggccaacct
     4441 ctcggcgctg atcgccccga ccgaaggctt tacgctggcc aacccgagcg gcgcgccggc
     4501 cgtcggcgat accttcctga tccggccgac ccgcgacatc gccgacaact tcacgctcaa
     4561 cgcggccatc gtttccgatc cgcggcggat cgccgcagcg ctgccggtgc gcagcgaaac
     4621 cggcgcgacc aatctcggct cggggcggat caccgtcgaa tcgctggtcc ccggctacgt
     4681 cgcgcccgcc gccgcctcgc cgttcacgct gacttacgac gtggcgacca atgccttcac
     4741 ggtcggcggc ctggccgccg gcactacggt cgccgttacc acgccgagcg cgaccacggt
     4801 gacgacactg ccggcgaccg tcccctacgc cgccgatgcg ctgtacacgg tcaacggcat
     4861 tacgttccgc ctctcgggtt cgcccggcca gggcgacagt ttccgtctcg aggccaacac
     4921 cgccggcatc gccgacgccc gcaacgcgct ggcgctcggc gaactgcaga cgcagcagac
     4981 catcgccggc ggtaccacca acttccagag cgcctacgcg cagctggtca gcagcgtcgg
     5041 caacaagtcg cgcgagattc gcgtcacccg tgacgcgcag accgcgctgc gcgatcaggc
     5101 agtggcctcg cgcgaagcga tgtcgggggt caacctcgac gaggaggccg ccaacctgct
     5161 gcgctatcag caggcctacc aggcggcagc ccgcgttatg caggtcagcg gcacgctgtt
     5221 tgaagaattg ctgtcgatcg gccgttaatg gggtgcaagg agagcgaaca tgcgaatcag
     5281 caccaaccag atctatagcg ccggcaccga gaacatcaac cgcaaccaga gcgaactgct
     5341 gcgcctgcag aaccagatat cgaccggccg ccgcatcctc cgtcccgagg atgacccagt
     5401 cgcagcggcg caggcgctgg ttgccaccca gagccagcaa atcaaccgcc agcatcagga
     5461 gaaccagggc aatgcccgtg cccagctagg cctgctcgac ggtcagctcg aatcgctcgg
     5521 caacgttatc caggacgtgc gcgagcgcac catccagttg ggcaatgccg cgctcaccga
     5581 ccgcgaacgc ggtttcatcg ccgaggagct ggagagccgc cttgagcaga tgctgtcaat
     5641 tgccaatgcg caacacggcg aggggcttta cctgttcgcc ggtttccagg gcgctagcgt
     5701 gccgttcgct cgggcgccgg gggccaccgg cccgttcaac gccgccaacc cgctggtgaa
     5761 cttcctcggc gatcagggcc agcgctcgct gcaggtcgat ggctcgcggc agatgccggt
     5821 gaatatctcc ggccttgacc tgttcatgaa catccttaat ggcaatggca ccttcaccac
     5881 cagcgcctca gccaccaatg gcggaactgg catcgccgat gccggcaccg tcaccaacgt
     5941 cgcgttgtgg aacgcctcga cagtgcaacc gcaggacttc cgcgtcgagt tcggcgtcga
     6001 taccacggtc aatccgaacg tcctcaagta caacctgatc gacaacgcca gcggcaactc
     6061 gatgttcacc aacgccgccc ccggcacgcc cgacaccgct ggctggcagg tcttcacgcc
     6121 ggggcaggcg atcgtctt
//
LOCUS       DATDAA010000135         9372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_324095,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000135 DATDAA010000000
VERSION     DATDAA010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324095"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VIS73_04310"
     CDS             complement(<1..820)
                     /locus_tag="VIS73_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY5762412.1"
                     /translation="MNLPLHVQAAYLLASILFIVGLKRMSSPATAKAGIRWAGFGMVL
                     ATAATFFVPGLQNLALIVVALALGALPAWWSAQRVQMTDMPQMVAIYNGMGGGSAAAI
                     AAVEIARGAGAHGASAMALGVIGAMIGAVSFAGSCIAWAKLQGVMKKATRLPAQNVVN
                     VVLALVVAVLAAYIAGIGQPSALLLGIFFLLALLLGLVVTLPIGGADMPVVISLFNAF
                     TGLAVGFEGYVLGNPALIIAGIVVGAAGTLLTQLMAKAMNRPISNILFTPMVAAG"
     gene            complement(817..1116)
                     /locus_tag="VIS73_04315"
     CDS             complement(817..1116)
                     /locus_tag="VIS73_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEY5762413.1"
                     /translation="MDGLTALYIFMLAAFTGYEIIMRVPVILHTPLMSGSNFVHGVVV
                     VGAMISLGGATTPFEQAMGFIAVLLGAANAAGGYVVTVRMLAMFKTGKSRAGGHA"
     gene            complement(1207..2334)
                     /locus_tag="VIS73_04320"
     CDS             complement(1207..2334)
                     /locus_tag="VIS73_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEY5762414.1"
                     /translation="MPLTIGIAGEIAADEKRVAIVPEVAKKLGDLKVDVAIERLAGLA
                     SHYKDSDYPAHVGWKHSAVEVFAASQLIWTVQPPSAERITQLPRGTIVAGLLQPYADR
                     ERLEQLCAQGITSLAMELVPRISRAQTMDALSSQAACAGYQCALIAAARSTKFFPMLT
                     TAAGTIRPARVFVIGAGVAGLQAIATARRLGAIVEAYDVRAAAREQIESLGAKFLDTG
                     VTAEGSGGYARELTVEEKAAQQEKLAKALATADCIITTAAIPGKKAPVIVTRDMVARM
                     KFGAIVVDMAAESGGNCELTVPGEHVVANDVHIHGPLNLPSRMPTHASEMYAKNLFNL
                     FSPFIKDGELKLDFEDEVIAGCLLTHEGKVVHENVRKVLDL"
     gene            complement(2478..3536)
                     /gene="fba"
                     /locus_tag="VIS73_04325"
     CDS             complement(2478..3536)
                     /gene="fba"
                     /locus_tag="VIS73_04325"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289321.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY5762415.1"
                     /translation="MALVSLRQVLDHAAENGYGVPAFNVNDLEQIQAIMQAARETDSP
                     VILQASAGARKYAGEAFLRKLVEGAVESYPDIPIVMHQDHGASAAVCMQAMRSGFSSV
                     MMDGSLMEDAKTPSTYEYNVEVTRRVCDMAHAIGVSVEGELGCLGSLETGDAGEEDGV
                     GAAGKLTHEQMLTDPEQAADFVARTGVDALAIAIGTSHGAYKFSRKPTGDILAISRVK
                     EIHARIPDTHLVMHGSSSVPQEWLAIIREFGGNLKETYGVPVEEIQTAIKHGVRKINI
                     DTDIRLAMTGAVRRHLAQKPDDFDPRGFLKPAIAAASGICKDRFEAFGCAGMASRIKP
                     VPLEAMVSRYQKRLSAAA"
     gene            complement(3574..5643)
                     /gene="tkt"
                     /locus_tag="VIS73_04330"
     CDS             complement(3574..5643)
                     /gene="tkt"
                     /locus_tag="VIS73_04330"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY5762416.1"
                     /translation="MPTRKIRRRCAHALRFLAIDAVQKAKSGHPGMPMGMADIAEVLW
                     RRHLRHNPGNPNWCGRDRFVLSNGHGSMLIYALLHLCGYELSIDELKRFRQLHSKTAG
                     HPEYGLTPGVETTTGPLGQGLANAVGMAVAERILGETFNRDDHVIVDHHTYAFVGDGC
                     LMEGVSHEACSLAGRLGLAKLVVFYDDNDISIDGHVQPWFADDTPKRFEAYGWRVVAN
                     VDGHDPDAIEAAIRIAKSQNQKPTLICCKTMIGFGSPNKAGSHDVHGAPLGDTEIEAS
                     RRLLEWPYAPFEIPDDVRKAWDSREEGRKAEALWDERFAAYRRAFPDLAAEFERRVLA
                     NGGRGMLPEDWLETANALLAGIERDAPTIASRKASQNVIEAIAPTLPELLGGSADLTG
                     SNLTNWSGTRPANSVAAGNYLQYGVREFGMTAIANGIALHGGFIPYTASFLVFSDYAR
                     NAIRMAALMKLRQLLIYTHDSIGLGEDGPTHQPIEHLASLRLIPGLDVWRPADAAETA
                     QAWIAGIERADGPTLLALSRQNLQAIARSRAENAAIRRGGYVIADAEQAEVVIVATGS
                     EVAIALDAQRELHAGGIAARVVSMPCTAVFERQDPAWRDSVIPADLPVVTIEAAHPEG
                     LRRYAGRDGVAIGIERFGESAPAGQLYEYFGITVAAVAAAGRNLSARARACEKSGKPT
                     AAVASTH"
     gene            complement(5665..6537)
                     /locus_tag="VIS73_04335"
     CDS             complement(5665..6537)
                     /locus_tag="VIS73_04335"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEY5762417.1"
                     /translation="MSAKHPIVVVTGSSGAGTTSVTRSFQHIFRREKLNAAIIEGDSF
                     HRFDRKAMKATMAAEAKAGNINFSHFGPEANLLAELEGLFRQYGESGTGKQRHYVHDA
                     EEAVRFAADPGTFTDWEDLPAETDLLFYEGLHGAAKHGDIDVGAHADLKVGVVPIINL
                     EWIQKLHRDKTQRGYSTEAVVDTVLRRMPDYINYICPQFSRTHINFQRVPTVDTSNPF
                     IAEDIPSPDESILVIRFADPKGIDFPYLLSMLHDSWMSRPNVICCPGGKMSLAMQVIL
                     TPLILQLMDRRRRL"
     gene            complement(6605..7681)
                     /locus_tag="VIS73_04340"
     CDS             complement(6605..7681)
                     /locus_tag="VIS73_04340"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEY5762418.1"
                     /translation="MLFGRTNVNKFLVESQRKNPDLAGDISMLISDVVRSCKTISHGV
                     SRGALAGLLGDDGAMNVQGETQKVLDVMSNNAFLHHCEWGGHVAAMASEEMEDIYRVP
                     PEHPRGRYLLVFDPLDGSSNIDVNLTIGSIFSILRCPEDCKDPTEKDFLQPGTRQVAA
                     GYALYGPSTMLVLTVGDGTHGFTLDRGIGEFLLTHPNLRVAEDTREFAINVSNERFWE
                     PPMQRYIDECLAGRTGPREKDFNMRWVASLVAEVHRILVRGGVFMYPRDNKDPDMPGR
                     LRLMYEANPMAMIIEQAGGQASTGRQSLREVVPTDLHQRVPVILGSKNEVTRIERYHA
                     EYDSGTDKPFTSPLFGTRTLFLDA"
     gene            7828..8742
                     /locus_tag="VIS73_04345"
     CDS             7828..8742
                     /locus_tag="VIS73_04345"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5762419.1"
                     /translation="MRNATVRQLQIFSVAAANLSFARAAEQLHLTHAAISLQIKQLEE
                     VAGIELFERIGKRVFLTEAGEIMLDYARQILNSLKEADEALSAIKGLKGGRVAVAVTS
                     TAEYFAPGLLAAFRRVHPEVGIRLLIDNRESVSRLLSRNEVDVAIMGRPPADFDAAAE
                     AFAPHPLVVIAAADHPLAKRKQVRADDLAGETLIVRESGSGTRSAMEGFLADHGVVLP
                     VGMEMGSNEAIKQAVVAGLGVSFISLHTLGLELKNKRLAVLKVEGTPVMRRWHIVRHR
                     SKRLTPALGAFWEYVLAFAPDQLKQLGK"
     gene            complement(8755..>9372)
                     /locus_tag="VIS73_04350"
     CDS             complement(8755..>9372)
                     /locus_tag="VIS73_04350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5762420.1"
                     /translation="VRSRARPLAAMGRVVGDRNARNALVFMALVIASGFTIIPFITIY
                     LVSNVGINQTQVPLIYLFGGLASFFSSRWIGRLADRHGKRRVYRTVAFAALLPLLAIT
                     HLPPVPITIVLIVTTAFFVLVPGRMVPAVAIVTAAVRPELRGTFMSLAASMQQLASGA
                     AAFIGGLIVTTGDDGRLLRYDLAGYVALTVTLAAIYAVRRIEIRN"
BASE COUNT         1571 a         2837 c         3242 g         1722 t
ORIGIN      
        1 cgccggcagc aaccatcggc gtgaacaaga tgttgctgat cggccggttc atcgccttgg
       61 ccatcaattg cgtgagcagc gtgccggcgg cgccgacgac gatgccggcg atgatcagcg
      121 ccggattgcc cagcacgtaa ccctcgaagc cgaccgctag cccggtgaag gcgttgaaca
      181 gcgagatcac cactggcatg tcggcgccgc cgatcggcag cgtgacgacg aggccgagca
      241 gcagcgcgag caggaagaag atgcctaaca gcagcgccga gggctggccg attccggcaa
      301 tgtaggcggc aagcacggcg acgacgagcg ccagtacgac attgacgacg ttctgcgccg
      361 gcagtcgagt cgccttcttc attacgccct gcagtttggc ccaggcgatg cacgagccag
      421 caaaggagac cgcgccgatc atcgcgccga tgacgccgag cgccatggcc gatgcgccgt
      481 gcgcgccggc gccgcgggcg atttccacag cggcgattgc cgctgccgag ccgccgccca
      541 tgccgttgta gattgccacc atctgcggca tgtcggtcat ctgcacgcgc tgtgccgacc
      601 accaggcggg cagtgcgccg agcgccagcg cgacaacgat cagcgccaga ttctgaagac
      661 cggggacgaa gaaggtggcg gcggtggcga ggaccatgcc gaagccagcc cagcggatgc
      721 ctgctttcgc cgttgctggc gaactcatgc gcttgaggcc gacgatgaag aggatggagg
      781 cgagcaggta ggctgcctgc acgtgcagtg gcaggttcat gcgtggcctc cggcgcggct
      841 cttgccggtc ttgaacatcg ccagcatgcg cacggtgacg acgtagccgc cggccgcgtt
      901 ggccgcgccg agcagcacgg cgatgaatcc cattgcctgc tcgaagggcg tggtcgcccc
      961 gcccagcgaa atcatcgcgc cgacgacaac gacgccgtgg acgaagttgg aacccgacat
     1021 cagcggcgtg tgcaggatca ccggcacgcg catgatgatc tcgtagccgg tgaaggcggc
     1081 gagcatgaag atgtagaggg cggtcagtcc gtccatgtca gcctcggcag catgcggcca
     1141 gcgagccggg atgcggccgc tgaagccttg ccccgttgcg ggggcggttg gtcagggaac
     1201 aggcggtcat agatcaagca ctttccgcac gttctcgtgg acgactttcc cttcatgcgt
     1261 gagcaggcag ccggcgatga cctcgtcttc gaagtcgagc ttcagttcgc cgtccttgat
     1321 aaagggtgag aacaggttga agaggttctt cgcgtacatc tccgaggcat gtgtcggcat
     1381 gcgcgagggc aggttgagtg ggccgtggat gtgtacgtcg ttggcgacga cgtgttcgcc
     1441 gggaacggtc agctcgcagt tgccacccga ctcggcggcc atgtcgacga cgatggcgcc
     1501 gaacttcatg cgcgcgacca tgtcgcgtgt gacgatcacc ggggccttct ttcccggaat
     1561 ggccgcggtg gtgatgatgc agtctgcggt ggcgagtgcc ttggctagct tctcctgctg
     1621 cgcggccttc tcctccaccg tcaactcgcg cgcgtatcca ccgctgcctt cggcggtgac
     1681 tccggtgtcg aggaattttg cgcccagcga ctcgatttgt tcgcgtgccg ccgcgcgcac
     1741 gtcgtaggct tcgacgatcg cgccgagacg ccgggcggtg gcaatcgcct gcaggccggc
     1801 aacgccggcg ccgatgacga agactcgcgc cgggcggatg gtgccggccg ccgtggtcag
     1861 catcggaaag aacttggtcg agcgggccgc ggcaatcagc gcgcactggt agccggcaca
     1921 ggcggcctgc gaggagagcg catccatggt ctgcgcgcgc gagatgcgcg gcaccagttc
     1981 cattgccagg ctggtgattc cctgtgcgca gagctgttcg aggcgctcgc gatcggcgta
     2041 gggctggagg aggccggcga cgatcgtgcc acgtggcagc tgagtgatcc gctcggcgct
     2101 tggtggttgc accgtccaga tgagctgcga tgcagcgaag acctcgacgg ccgagtgttt
     2161 ccagccgacg tgcgccggat agtcgctgtc cttgtagtgg ctggcgagtc ccgcgaggcg
     2221 ttcgatggcg acgtcgacct tgaggtcgcc gagcttcttc gccacttcgg ggacaatcgc
     2281 cacacgcttt tcgtcggccg cgatctcgcc agcgatgccg atggtcaggg gcattggagg
     2341 tgatcctctt tgttcttggg taaggcgggt tgccggccgg cagcaacccg ccctcagtaa
     2401 aaatcaatgc gccggattgt gccacccccc aaagggagga gagcgcctgc cgccggcgcg
     2461 gcgggcttgc ggatacatca ggccgcggcg ctcagccgct tctggtagcg ggagaccatc
     2521 gcctccagcg gaacgggttt gatgcgactg gccatgccgg cgcagccgaa ggcctcgaaa
     2581 cggtccttgc agatgccgct tgcggcagcg atcgccggct tgaggaaacc gcgcgggtcg
     2641 aagtcgtcgg gcttctgcgc caggtggcgg cgcacggcac cggtcatcgc caggcggatg
     2701 tcggtgtcga tgttgatctt gcgcacgcca tgcttgatcg cggtctgaat ctcctcgacc
     2761 gggacgccgt aggtttcctt caggttgccg ccgaattcgc gaatgattgc cagccattcc
     2821 tgcggcactg aactcgatcc atgcatgacc aggtgcgtgt cgggaatgcg cgcatggatt
     2881 tccttgacgc gcgagatcgc cagaatgtcg ccggtcggct tgcgcgagaa cttgtaggcg
     2941 ccgtggctgg tgccgatggc aatcgccagg gcgtcgacgc cggtgcgggc gacgaagtcg
     3001 gccgcctgct cgggatcggt gagcatttgc tcgtgcgtca gcttgccggc ggcgccgacg
     3061 ccatcctctt cgccggcatc gccggtctcg agcgagccga ggcagccgag ttcgccctcg
     3121 accgagacgc cgatcgcatg cgccatgtcg cagacgcggc gtgtgacctc gacgttgtac
     3181 tcgtaggtcg acggggtctt ggcgtcctcc atcagcgagc catccatcat caccgaggaa
     3241 aagcccgagc gcatcgcctg catgcacacg gccgcgctgg cgccgtggtc ctgatgcatg
     3301 acgatgggga tgtcggggta gctttcgacg gcgccttcga cgagtttgcg caggaaggct
     3361 tcgccggcat acttgcgggc gccggccgag gcctgaagga ttaccgggct gtcggtctcg
     3421 cgggcggcct gcatgatcgc ctggatctgt tcgaggtcgt tgacattaaa ggcgggcacg
     3481 ccgtagccgt tttcggcggc atggtcgagc acctgtctga gcgaaacgag ggccatagtc
     3541 tgtctcctga gtcagttagt tggattgggc gaatcagtgg gtcgaggcga ccgcagcagt
     3601 aggcttgccg ctcttctcgc aggcccgtgc gcgtgcggat aggttgcgac ctgctgcagc
     3661 gaccgcggcg acggtgatgc caaagtactc gtagagttgg ccggccggtg ccgattcgcc
     3721 gaaacgttcg atgccgatgg cgacaccgtc gcgtccggcg tacctgcgca gtccttcggg
     3781 gtgcgcagcc tcaatggtga ccaccggcag gtctgctggg atcacgctat cgcgccaggc
     3841 ggggtcctgt cgttcgaaaa ccgcggtgca gggcatcgac accacgcggg ccgcgatgcc
     3901 gccggcgtgc agttcgcgct gggcgtcgag ggcgatggcg acctcggagc ccgtcgcgac
     3961 gatgaccact tcggcttgct ccgcatcggc gatcacgtag ccgccgcgac ggatcgctgc
     4021 gttctcggcg cggctgcgcg cgatcgcttg caggttctgc cgtgacagcg cgagtagggt
     4081 cgggccgtcg gcacgttcga tgccggcaat ccaggcctgc gcggtttccg cagcgtcggc
     4141 cgggcgccag acatcgagcc cgggaatcag gcgcaggctc gccagatgct cgatgggctg
     4201 gtgcgtcggt ccgtcttcgc cgagcccgat cgaatcgtgg gtgtagatca acaattggcg
     4261 cagcttcatc agcgctgcca tgcgaatcgc gttgcgcgcg tagtcggaaa agacgaggaa
     4321 gctggcggta tatggaatga atccgccgtg cagcgcgatg ccgttggcga tggcggtcat
     4381 gccgaactcg cggacgccgt actgcaggta gttgccggcg gcgaccgagt tggcgggacg
     4441 cgtgccgctc cagttggtga ggttcgaccc tgtgaggtcg gccgagccgc caagcagttc
     4501 gggcagcgtc ggcgcgatgg cttcgatgac gttctgcgag gccttgcgcg aggcgatcgt
     4561 cggtgcgtcg cgttcgatgc cggcgagcag cgcgttggcg gtttccagcc agtcttcagg
     4621 gagcatgccg cgaccgccgt tggcgaggac gcgtcgctcg aattcggcgg cgaggtcggg
     4681 gaaggcgcgg cgataggcgg cgaagcgctc gtcccatagg gcctcggcct tgcggccttc
     4741 ctcgcgcgaa tcccaggcct tgcgcacatc gtcgggaatc tcgaagggcg cgtacggcca
     4801 ttccagcagt cgacggctcg cctcgatttc agtgtcgccg agcggtgcgc cgtgcacgtc
     4861 gtggctaccg gccttgttcg ggctgccgaa gccgatcatc gtcttgcagc agatcagcgt
     4921 cggcttctgg ttctggctct tggcgatacg gatcgccgct tcgattgcgt ctgggtcgtg
     4981 gccatcgacg ttggcgacga cgcgccagcc ataggcctcg aagcgtttcg gcgtgtcgtc
     5041 ggcgaaccag ggctggacgt ggccgtcgat cgagatgtcg ttgtcgtcgt agaagacgac
     5101 cagcttcgcc aaccccaggc ggccggccag cgagcaggcc tcgtgcgaga cgccctccat
     5161 caggcagccg tcgccgacga aggcgtaggt gtggtgatcg acgatgacgt ggtcgtcgcg
     5221 gttgaaggtc tcgccgagga tgcgttcggc aacggccatg cctaccgcgt tggcgaggcc
     5281 ctggccgagc ggaccggtag tcgtttcgac gcccggggtc aggccgtact cgggatgccc
     5341 ggcggttttc gagtgcagct ggcgaaagcg cttcaactcg tcgattgaca gctcatagcc
     5401 gcagagatgc agaagcgcgt agatcagcat cgagccgtgg ccgttggaga ggacgaaccg
     5461 gtcgcggccg caccagttag gatttccggg gttatgtcgg agatggcggc gccacagtac
     5521 ttcggcgatg tcggccatgc ccatcggcat tcccggatgc ccggacttgg ccttttgtac
     5581 cgcatcgatg gcaagaaaac gcagcgcgtg ggcgcagcgg cggcggattt tcctggtcgg
     5641 catggatggt ttcctccgct ttgttcagag ccggcgccgg cgatccatca gctgcaggat
     5701 cagcggcgtg agaatcacct gcattgccag gctcatcttg ccgcccgggc agcagatgac
     5761 gttcggccgt gacatccagg agtcgtgcag catcgacagc agataaggaa agtcgatgcc
     5821 cttcggatcg gcgaaacgga taaccaggat gctttcgtcg ggcgagggga tatcctcggc
     5881 gatgaaaggg ttcgaggtat cgacggtcgg cacgcgctga aagttgatgt gcgtgcgcga
     5941 gaactgcggg cagatgtagt tgatgtagtc gggcatgcgc cgcagcacgg tatcgaccac
     6001 cgcctcggtc gagtagccgc gctgcgtctt gtcgcgatgc agcttctgga tccactcgag
     6061 gttgatgatc ggcacgacgc cgaccttgag gtcggcgtgg gcgccgacgt cgatgtcgcc
     6121 atgtttggca gcaccgtgca ggccttcgta aaagagcagg tcagtttccg cgggcaggtc
     6181 ttcccagtcg gtgaaggtgc cgggatcggc ggcgaagcgt acggcttctt cggcgtcgtg
     6241 cacgtagtgc cgctgcttgc cagtgccgct ttcgccgtac tgccggaaca gtccttcgag
     6301 ttcggcgagc aggttcgcct cggggccgaa gtggctgaag ttgatgtttc cggccttggc
     6361 ctcggccgcc atcgtggctt tcatcgcctt gcggtcgaag cgatggaaac tgtcgccttc
     6421 gatgatcgcg gcgttgagtt tctcgcgacg gaagatgtgc tggaacgagc gcgtcaccga
     6481 agtggtaccg gcgccggacg aaccagtcac cacgacgatc ggatgttttg cggacatgcg
     6541 tgcctccccg gatagacgaa atgggtcgtt gttgtagtga tacgtgttgt ggcggtgacg
     6601 ggcctcaggc gtcgaggaac agcgttcgcg tgccgaacag cggtgacgtg aacggtttgt
     6661 cggtccccga gtcgtattcg gcgtggtagc gctcgatgcg cgtgacctcg ttcttcgagc
     6721 cgagaatcac cggcacccgc tggtgcaggt cggtggggac cacctcgcgc aggctctgcc
     6781 ggccggtcga tgcctggccg ccggcttgct cgatgatcat cgccatcggg ttggcttcgt
     6841 acatcagccg caggcggcct ggcatgtccg ggtccttgtt gtcgcgcggg tacatgaaga
     6901 cgccgccgcg aacgagaatt cgatggacct cggcgaccag cgaggcgacc caccgcatgt
     6961 tgaagtcctt ctcgcgcggg ccggtccggc cggctaggca ctcgtcgatg tagcgctgca
     7021 tcggcggttc ccagaagcgc tcgttggaga cgttgatcgc gaactcgcgg gtgtcctcgg
     7081 cgacacgcag gttggggtgg gtgagcagga attcgccgat gccccggtcg agcgtgaatc
     7141 cgtgtgtgcc gtcgccgacg gtcagcacca gcatcgtcga tggcccgtag agcgcgtagc
     7201 cggcagccac ctgacgggtg ccgggttgca ggaagtcctt ttcggtcggg tccttgcagt
     7261 cttcggggca gcgcaggatc gagaagatac tgccgatcgt caggttgacg tcgatgttcg
     7321 acgaaccgtc gagcggatcg aatacgagca ggtagcggcc gcgcggatgc tcgggcggga
     7381 cacggtagat gtcctccatt tcctcggagg ccatcgccgc gacgtggcca ccccattcgc
     7441 agtgatgcag gaaggcgttg ttcgacatga cgtcgagcac cttctgcgtt tccccctgca
     7501 cgttcatcgc gccgtcatcg ccgagcaggc cggccaacgc gccgcgggaa accccatgcg
     7561 aaatcgtctt gcacgagcgg acgacgtcgc tgatgagcat cgaaatgtcg ccagcgagat
     7621 ccgggttctt gcgctgggac tcgaccagaa acttgttgac gttggtgcgt ccgaatagca
     7681 tggttcctcc tctgtgcggc gatggtctgg ccgggtctat gcccgttgga tcgcgtgctt
     7741 gcgcttcacg cctgactcga tattagggaa acgatacaaa aatgctagtt aaacattttt
     7801 gtttaatacg taagtgaaag attgcctatg agaaacgcga ccgttcgcca gttgcagatc
     7861 ttttccgtcg ccgccgcgaa cctctccttt gcccgcgcgg cagagcaact gcacctcacg
     7921 catgcggcga tatcgctgca gatcaagcag ctcgaggagg tcgccggaat cgagttgttc
     7981 gagcggattg gcaagcgcgt cttcctcacc gaggccgggg aaatcatgct cgactacgct
     8041 cggcagattc tcaattcgct gaaggaggcc gacgaggcgc tgtcagcgat caagggcctg
     8101 aaaggcgggc gggtagcagt cgccgtgacc agtaccgccg agtacttcgc gccgggcctt
     8161 ctggcggctt tccgccgcgt ccatcccgag gtcggcatac gcctgttgat cgacaaccgc
     8221 gaaagcgtgt cccgtctact gtcgcgcaac gaggtcgacg tcgcaatcat gggccggccg
     8281 cctgccgatt tcgacgctgc ggccgaggct ttcgcaccgc atccgctagt cgtcattgcc
     8341 gctgctgacc atccgctggc gaaaaggaag caggtgcgcg ccgacgatct ggccggagag
     8401 acgctaattg ttcgcgagag cggttcgggc acgcgctcgg cgatggaggg ttttcttgcc
     8461 gatcacggtg tcgtgctgcc ggtcggcatg gagatgggat cgaacgaggc gatcaagcag
     8521 gccgtggtgg cggggctcgg tgtctccttc atctcgctgc acacgctcgg actcgagctg
     8581 aagaacaagc gtctcgccgt actcaaggtc gagggcacgc cggttatgcg gcgctggcat
     8641 atcgtccgcc accgtagtaa gcgcctgact ccggcgcttg gtgccttttg ggaatacgtg
     8701 ctggcctttg cgcccgacca gctcaagcag ctcggcaaat gaccgctggc tgcctcagtt
     8761 gcgaatctcg attcgccgga cggcgtagat tgccgccaag gtgaccgtca acgccacgta
     8821 gccggccagg tcgtaacgca gcagccggcc gtcgtcgccg gtggtgacga tcaagccgcc
     8881 gatgaacgcc gctgcgcctg aggccagctg ttgcatcgag gcggccagcg acatgaaggt
     8941 accgcgcaac tcagggcgca ccgccgcagt gacgatggcg actgccggca ccatgcggcc
     9001 aggcacgagt acgaagaacg cagtcgtcac gatcagcacg atggtgatgg gtaccggcgg
     9061 cagatgcgtg atcgccagca acggcagcag cgcggcgaag gcaaccgtgc gatagacgcg
     9121 gcgcttgccg tgtcgatcgg cgaggcggcc gatccagcgc gacgagaaga acgaggcgag
     9181 cccgccgaac aggtagatca gcggcacctg cgtctggttg atcccgacgt tgctgaccag
     9241 atagattgtg atgaagggga tgatcgtgaa gcccgaggcg atcaccaatg ccatgaatac
     9301 cagcgcattg cgggcgttgc ggtcgccgac cacacggccc atcgccgcca gcggccgggc
     9361 gcggctgcgc ac
//
LOCUS       DATDAA010000136        10880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_394276,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000136 DATDAA010000000
VERSION     DATDAA010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10880
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394276"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..543
                     /locus_tag="VIS73_04355"
     CDS             127..543
                     /locus_tag="VIS73_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762421.1"
                     /translation="MPETETLPVGPIPRESLVRLTHIIYALHACAVVIGVIGAATIAG
                     AFVFGLPSLLAVVLNYVKRDDVRGTWLDSHFSWQIRTFWFTAMWLAIYFLLVFTIIGI
                     PAAIVLIAVLGIWVMYRVIRGWLALNDARPVTPLNT"
     gene            complement(556..1530)
                     /locus_tag="VIS73_04360"
     CDS             complement(556..1530)
                     /locus_tag="VIS73_04360"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEY5762422.1"
                     /translation="MPMTLRRHIAPGLTAAVLATILATGCAGIATLTEPSRLALTEHP
                     LVNAIARSDGKRADEAAVLAAMRRADVIILGETHDNPEHHAIQARLLTELTKSGRRPA
                     VVFEQIDRTRQDDLDRAQRLPAGSARIEAIGATFAKGWDWPAYRPLVEIAVDAQLPIR
                     GGNVDRSALRPLVKEGWETIAEGIRNHLAIDAAWNPERDAYLTKVIDETHCNAIDNAL
                     RDGLVRAQRLRDATIADAVLSARGYESRPVVLITGRGHARSDAGVPRYVAAREPALRV
                     VSIGISEVEAGAATFAPYLKDRADGVPHDFLWLTARFDRPDPCAAFGR"
     gene            1609..3636
                     /locus_tag="VIS73_04365"
     CDS             1609..3636
                     /locus_tag="VIS73_04365"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HEY5762423.1"
                     /translation="MTAFPHLLAPLDLGFCTLKNRVLMGSMHTGLEESPQGFERLAAF
                     YERRAQGGVGLIVTGGIAPNKAGAVFPGAARLDSPRHLDGHRLVTDAVHSAGGMIALQ
                     ILHAGRYAYHPGAVAPSPLQAPISPAMPHELSDAEVEATIADYVRCAELAREAGYDGV
                     EIMGSEGYLINQFTAPRTNKRSDRWGGSLENRLRLPTEIVRRVREAVGRDFIIIYRLS
                     MLDLVEDGLAWDEIIAQAKAVEAAGASIINTGIGWHEARVPTIATMVPQGAFTWVTAK
                     LKADLRGAVSLPLVATNRINSPQQAEAILAAGGADMVSLARPFLADPDFVAKAAAEDA
                     DAINTCIACNQACLDHIFEAKACSCLVNPLACREFDFHISLATKKKRIAVVGAGPAGM
                     AAAAVAAERGHAVTLFDSAESIGGQFNLARRIPGKAEFATTLRYFGVRLHRAGVTLKL
                     GTRARAEDLAGQFDHVLLATGIAPRTPAIPGIDHGRVANYVDIVSGRRNAGARVAIIG
                     AGGIGFDVAELLTHGGAADDDIADYQQRWGIDPKVAQRGGVTRPIPEKPERQVWLLQR
                     KESKVGDGLAKTTGWIRRATLARRGVQMLAGVAYERIDDAGLHIRIGGQTQLLPVDTV
                     VVCAGQEPLRELEAPLAAAGVPVTRIGGADVAAELDAKRAIRQGTEVAAQL"
     gene            complement(3643..4674)
                     /locus_tag="VIS73_04370"
     CDS             complement(3643..4674)
                     /locus_tag="VIS73_04370"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY5762424.1"
                     /translation="MRSPSASRVAPASYRLVALAALALFGACSDTPRSTVDDRAGLLA
                     PAERAQIETFHGLLLADHDIDYHVVTAVDAGDLSHAAIDTFAARQVGIASKTGRGLLL
                     LIDPAQNQVRLEVSAALEGVYVDAFVAYLEQRQMVPFFQRKRIADGILATTELIVERA
                     MRAQRQAGFDGEVWTAFSAGGGATSEAGIGQAPSTSTRGAAIVARGDEPAAVVQAYLD
                     AMSARNANPNSAIFSNATREMLAKWVVTPAQMDNLARTYRDCHAEAPRIVADRAVIRY
                     APAERACAPWFLIKEDGDWRLDLTMMQGSIRFGRNNAWHFTPGAVHPYRFAFADWRFD
                     ANGFPLIPD"
     gene            complement(4652..5557)
                     /locus_tag="VIS73_04375"
     CDS             complement(4652..5557)
                     /locus_tag="VIS73_04375"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY5762425.1"
                     /translation="MDYQERVERLVAAGAISAGQAEALLEGIGNEPPAPASRPRRTVW
                     ILLAAAALTTALALAALLGGVAAEPAGIQNVAETLNQPGSIGTMNRTVGVVAAIGLLL
                     VVPLLIVAFSYNSLVAREEAVFSAWSDVESTYQRRSDLIPQLVESVSRYLKHESETVA
                     AVAEKRTTPLRDAVDQVLARQADNRAALSALGSDPTGDEGALTRLSQSDQALGQALQG
                     FRAVAEAYPELKSGDQFLELQAQLEGTENRINVARLRFNDAAQSFNTAMRQLPVSLIA
                     GLGGFQRKAYFKADAGSARAKPLGL"
     gene            complement(5650..6144)
                     /locus_tag="VIS73_04380"
     CDS             complement(5650..6144)
                     /locus_tag="VIS73_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762426.1"
                     /translation="MKRAITTLLLAGATTAALAQMGMGGGMGNHMHHGMPMGEAAKPA
                     PETRQAIVLTADERAIVLAEMRGFMESVQGVLAAVAANDAKALADAAGKSGMKVMGHL
                     PMATRAKFPQGLMMMGRATHMGFDELATAAAGGTVPADTIDRLATITGQCVACHATFR
                     FEVQ"
     gene            6343..7053
                     /locus_tag="VIS73_04385"
     CDS             6343..7053
                     /locus_tag="VIS73_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5762427.1"
                     /translation="MIDLYTAGTPNGHKVSIALEELALPYELHVLDLSAQQQKQPWFL
                     AINPNGRIPAIVDRDTDDFAVFESGAILVYLAEKTGRLLPADVKGRSRVLQWLMFQMG
                     GIGPMMGQANVFYRYFPEKIQPAIDRYQGESKRLFRVLDGHLADNEYLAGDYSIADIA
                     NWAWVRTHRWSGVEIDDLPHLRRWVEAIRARPAVQSGIDRPRSSIDSRLEDPAEADRF
                     AAEARKMVETGQSKAGGV"
     gene            7212..8408
                     /locus_tag="VIS73_04390"
     CDS             7212..8408
                     /locus_tag="VIS73_04390"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEY5762428.1"
                     /translation="MSLFRYPIFFAPHRFFFAAGFAQWAFLLIFWTLEMLARVGGNDL
                     PWSGIAPARWLHGALFVFGPFPLWIFAFAFTAMPRWLKLPPVLPREYLPGGLAIAAGW
                     LLADLALWGGSLRLMLIGFGTVGAGWGLLLMTLWRIARRAPTGDSHPRLLAGAVTLGV
                     PSLWLAVWGLSTAHAGRIDLALTLGVWAFLLPTFVVVAHRMLPFFTRSALPQVVPWQP
                     QWVLWCLPACLLAYGLSVRYTPTLAWLPAFAGLAISALVSMRWQLRASFANRLLATHH
                     VALVWLSVAFALGALQSALLAADILWGGIAPLHAVTFGFAVTMVFGMATRVTLGHSGQ
                     PIGATPLLWRLFWTLQLAVVLRLAADLLPRLAVVLLPLAALLSIVVFGAWLWQYASYW
                     AIDADE"
     gene            complement(8415..>10880)
                     /locus_tag="VIS73_04395"
     CDS             complement(8415..>10880)
                     /locus_tag="VIS73_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5762429.1"
                     /translation="NDIAEAIARGNLDAPAGRLHMGEQEIASRTTGRLASVAEIGGLP
                     IRLADGSFLRLAEIAEVVDAQEDERIRVRFNGTPGVRLAIQKQPTANTVAVADVVAAR
                     LADLHRRALIPADIAIDQVSDQAIYVRQSLANATYAALAGAVLAMTVVYAFLGSVRGT
                     LIIGSAIPISIMITFAIMALGGLSLNLMTLGGLALGVGMLVDNTIVMLENIERHQSQG
                     EAPLVAATGAAGEVTSAIIAATSTNLAAVLPFLFISGLVGLLFRELIFTITAAIVASL
                     IVAVTLVPALAARSRPPEPSRVTRAIQAMVARLQAIYRPLVTRTLAAPLAVAALALVL
                     LAGAAGLFWSLAGQQEFLPTMDDGRVQVQINTDTGRSLDDMDRDVRRIEAIALEVGEV
                     DGLSVIAGGSIFGRTARERPNRSTLAIQLVPAGQRNRTIQAWVGDFSRAVAKAELAGV
                     TVRARPSGIRGVRLARSDEEVSIRILGPELDELARIADRLVATLRGQPGLRNLAHSAE
                     ERRPEFAVTVDRTRAAELGVDVETVGRALAIALDGLVVGDFLDGDRAYDIRVRLPPRS
                     VDSPAALAAIIVGGGGARPPITIGDVASIGMISAPTEIRRENQRRIVEITAALTGEVP
                     LAAAIRAAQVTLAKAELPAGYSLDFGGAYETLERGKTLVATLAALALFLVFVVMTVQY
                     ESLKNPAIILAGVPFALIGVLIGLLATGLPLSMPVWLGVIMLIGVVVNNAIVLVEYIE
                     ILRARGEALLDAIVEAARLRLRPILMTTFTTVAGMLPLSLGLGEGSEMLRPLAVCMVF
                     GLSFSMLVSLLLIPSLYLLAHRR"
BASE COUNT         1620 a         3684 c         3727 g         1849 t
ORIGIN      
        1 cggctttcgt ccctataagc tcgacccggc cgcgggcgag gcgctgctgc tgcagaaaca
       61 actgtgagcg ctgcggcata atcgcagccg ccttagaccg ctgccgaggc agccttggag
      121 ttgctcatgc ctgaaactga aaccctgcca gtcggaccga ttccccgcga atcgctggtt
      181 cgcctgaccc acatcattta cgccttgcac gcctgcgccg tggtcatcgg cgtcatcggt
      241 gccgcgacca tcgccggcgc cttcgttttc ggcctgcctt cgctgctcgc cgtggtgctc
      301 aactacgtca agcgcgacga cgtgcgcggt acctggctcg acagccactt ctcgtggcag
      361 atccgcacct tctggtttac cgcgatgtgg ctggcgatct actttctgct cgtgttcacg
      421 attatcggca tcccggccgc gatcgtgctg atcgccgtgc tcggcatctg ggtcatgtac
      481 cgcgtgatcc gcggctggct ggcgctgaac gacgcacggc cggtgacacc gctgaatacc
      541 tgatcgaccg gtcggctacc ggccgaaggc cgcgcagggg tcgggccggt cgaagcgcgc
      601 ggtgagccag agaaagtcat gcggcacgcc gtcggcgcgg tccttgagat agggcgcgaa
      661 cgtcgctgcg ccggcctcga cttcgctgat tccgatcgac accacccgta gcgccggctc
      721 gcgcgccgcg acgtagcgcg gcacgccggc atcgctgcgc gcgtggccac gaccggtgat
      781 cagtaccacc gggcgcgatt cgtagccgcg ggcgctaagc acggcgtcgg cgattgttgc
      841 gtcgcgcagg cgctgggcgc gcacgaggcc gtcgcgcagg gcgttgtcga tggcgttgca
      901 gtgcgtctcg tcgatcacct tggtcaggta cgcatcgcgc tcaggattcc atgccgcgtc
      961 gatcgccaga tgattgcgaa tgccctcggc gatggtttcc cagccctcct tgaccagcgg
     1021 ccgcagcgca ctccggtcga cgttgccgcc gcggattggt agttgggcat cgacggcgat
     1081 ctctaccagc ggccggtagg ccggccagtc ccagcccttg gcgaaggtcg cgccgatcgc
     1141 ctcgatgcgg gcgctgccgg caggcagtcg ctgcgcccgg tcgagatcgt cctggcgtgt
     1201 gcggtcgatc tgctcgaata ccaccgccgg ccgccggcct gatttagtca gctcggtcag
     1261 cagtcgcgct tggatcgcgt ggtgctcggg gttgtcgtgt gtttcgccga ggatgatcac
     1321 gtcggcgcga cgcattgccg cgagcacggc cgcctcgtcg gcgcgcttgc cgtcgctgcg
     1381 ggcgatggcg ttgaccagcg ggtgctcggt cagtgccagc cgactcggct cggtcagcgt
     1441 ggcgatcccg gcacagccgg tagcgagaat ggtggccagt acagcggcgg tcagcccagg
     1501 ggcgatatgg cggcgaagcg tcatcggcat tcgcggactc cggtcagaat ggaaaacgag
     1561 gtggcatcat acggcgggtc cggctccgac cgcccccaga ggatgaacat gaccgccttc
     1621 ccgcacctgc tcgcccccct cgacctcggc ttctgcacgc tgaaaaaccg cgtactgatg
     1681 gggtcgatgc acaccgggct cgaagaatca ccacagggtt tcgagcggct cgccgccttc
     1741 tacgaacgcc gtgcccaggg cggcgtcggc ctcatcgtca ccggcggcat cgccccgaac
     1801 aaggccggcg ccgtcttccc cggcgcggcg cggctcgaca gcccgcgtca cctcgacggc
     1861 caccgtctgg tcaccgacgc ggtgcattcg gcgggcggga tgatcgcgct gcagatcctg
     1921 cacgccgggc gctacgccta ccatccgggc gccgtcgccc cctcgccgct gcaggcaccg
     1981 atctcgccgg cgatgccgca cgagctctcc gatgccgagg tcgaggcgac catcgccgac
     2041 tacgtgcgct gcgccgaact ggcgcgcgag gccggctacg acggcgtcga gatcatgggt
     2101 tccgagggtt acctaatcaa ccagttcacc gcgccgcgca ccaacaagcg cagcgatcgc
     2161 tggggcggca gcctcgagaa ccgcctgcgc ctgccgaccg agatcgtccg ccgcgtgcgg
     2221 gaggcggtcg gccgcgactt catcatcatc taccggctgt cgatgctcga cctcgtcgag
     2281 gacggcctcg cctgggacga aatcatcgcc caggccaagg ccgtcgaagc cgccggcgcc
     2341 agcatcatca acaccggcat cggctggcac gaggcgcgcg tgccgaccat cgcgactatg
     2401 gtgccgcagg gcgcgtttac ctgggtgacc gccaagctca aggccgatct gcgcggcgcg
     2461 gtctcgctgc cgctggtggc gaccaaccgc atcaacagcc cgcagcaggc cgaggccatt
     2521 ctcgctgccg gcggcgccga catggtctcg ctggcgcggc ccttcctcgc ggaccccgat
     2581 ttcgtcgcca aggctgccgc cgaagatgct gacgccatca acacctgcat cgcctgcaac
     2641 caggcctgcc tcgaccacat cttcgaagcc aaggcctgct cctgcctggt caacccgctc
     2701 gcctgccgag agttcgactt ccacatttca ctggcgacaa agaagaagcg catcgccgtc
     2761 gtcggcgccg gcccggccgg catggctgcc gccgcagtcg ccgccgaacg cggccacgcg
     2821 gtcacgctgt tcgattcggc cgagtccatc ggcggccagt tcaacctcgc ccgacgaatc
     2881 cctggcaagg ccgaattcgc gacgacgcta cgctatttcg gcgtccgcct gcaccgcgcc
     2941 ggcgtgacgc tcaagctcgg cacccgtgcc cgcgccgaag acctcgccgg ccagttcgac
     3001 cacgtgctgc tcgccaccgg catcgcgccg cgtacgccgg cgattcccgg catcgaccac
     3061 ggccgggtcg ccaactacgt cgacatcgtc agcggccgcc gcaatgccgg cgcgcgcgtc
     3121 gctatcatcg gcgccggcgg aatcggcttc gacgtcgccg aactgctcac ccacggcggt
     3181 gcggccgacg acgacattgc cgactaccag cagcgctggg ggattgaccc gaaagtcgcg
     3241 cagcgcggtg gcgtgacgcg gccgatcccg gaaaaacccg agcggcaggt ctggctattg
     3301 cagcgcaagg aaagcaaggt cggcgacgga ttggccaaga ctactggctg gattcgccgc
     3361 gccacgctgg cccgccgtgg cgtgcaaatg ctcgccggcg ttgcctatga gcgcatcgac
     3421 gatgccggcc tgcacatccg catcggcggc cagacgcagc tgctgcccgt cgacaccgtc
     3481 gtcgtctgtg ccggacagga accgctgcgc gaactcgaag cgccgctggc ggcagcgggc
     3541 gtgccggtga cgcgcatcgg cggtgccgac gtcgccgccg aactcgacgc caagcgggcg
     3601 atcaggcagg gcaccgaggt cgcggcacag ctataggcga tttcagtcgg ggataagcgg
     3661 gaagccgttg gcgtcgaagc gccagtcggc aaaggcgaat cgatacggat gaacggcgcc
     3721 tggggtgaaa tgccaggcat tgtttcgtcc gaagcggatg cttccctgca tcatggtcag
     3781 gtcaaggcgc cagtcgccat cttccttgat caggaaccag ggcgcacagg cgcgctctgc
     3841 cggcgcgtag cggattaccg cgcggtctgc cacgattcgg ggtgcttcgg cgtgacagtc
     3901 gcggtaggtc cgggcaaggt tgtccatttg cgctggcgtg accacccatt tcgccagcat
     3961 ttcgcgggtc gcgttgctga agatcgccga gttcggattg gcgttgcgcg ccgacatcgc
     4021 gtcgaggtag gcctgcacga cggcggcggg ttcatcgccg cgggcgacga tggccgcgcc
     4081 gcgcgtagac gtgcttggtg cttgcccaat tccggcttcg ctggtggcgc caccgccagc
     4141 cgaaaatgct gtccagactt ctccgtcgaa cccggcctgt cgctgggcgc gcatcgcccg
     4201 ctcgactatc agttcggttg tggcgaggat gccgtcggcg attcgcttgc gctggaagaa
     4261 gggcaccatc tgccgctgtt cgaggtaggc gacgaaggcg tcgacgtaaa cgccttcgag
     4321 cgctgccgag acttcgagcc gaacctggtt ctgggcgggg tctatcagca gcagcagccc
     4381 gcgccccgtc ttgctcgcaa tccctacctg gcgcgcggcg aaggtgtcga ttgcggcgtg
     4441 ggagaggtcg cctgcgtcga cagccgtaac cacgtggtag tcaatgtcgt ggtcggcgag
     4501 caggagcccg tggaaggttt cgatctgtgc gcgctcggcc ggggcgagga ggccagcgcg
     4561 gtcgtcgacc gtgcttcgcg gcgtgtcgct gcacgcgccg aacagcgcca gcgcggcgag
     4621 cgcgaccaga cgatacgaag ccggcgcgac tctagaggcc gaggggcttc gcacgcgcgc
     4681 tgccggcgtc ggccttgaag taagccttgc gctggaaacc gccgagaccg gcgatcaggc
     4741 tcaccgggag ctgacgcatg gccgtgttga acgactgcgc ggcatcgttg aagcgcaggc
     4801 gcgcgacgtt gatccggttc tcggtgcctt caagttgcgc ctgcagttca aggaactggt
     4861 cgcccgattt gagctcggga taggcctcgg cgacggcgcg gaatccctgc agcgcctgac
     4921 ccaaggcctg gtcgctctgc gatagcctgg tcagggcgcc ctcgtcgccg gtcggatcgc
     4981 tgcccaaggc gctgagcgcg gcacggttat cggcctgtcg tgccaacacc tgatcgacgg
     5041 cgtcgcgaag cggcgtcgtc cgtttctcgg caaccgccgc cacggtttcc gattcgtgct
     5101 tcagatagcg gctgaccgat tcgacgagct gggggatcag gtcactgcgc cgctggtagg
     5161 tgctttccac atccgaccac gccgagaaca cggcctcctc gcgggcgacg aggctgttgt
     5221 agctgaacgc cacgatcagc agcggcacca ccagcaacag gccaatggcg gcaacaacgc
     5281 cgacggttcg attcatcgtt ccaatactcc ctggttgatt gagcgtttcg gcgacgttct
     5341 gtattccggc gggctctgcg gcgacgccac caagcagcgc agcgagcgcg agcgcggtgg
     5401 ttagcgcggc ggcggcgagc agaatccaga ccgtgcggcg tggccgtgaa gccggcgctg
     5461 gcggctcgtt cccgatgcct tccaagagcg cttcagcctg ccctgcgctg atcgcgcccg
     5521 ctgcgaccaa tctttcgacg cgttcctgat aatccacgat gctgcctctc ccgagttact
     5581 gctgtcgccg gtcgcattta agcacagtgc atcgtgtcag tacggccgtg gaacggggcg
     5641 gcgacctgcc tactgcacct cgaagcggaa ggtcgcgtgg caggcgacgc actggccggt
     5701 gatcgtcgcc aagcggtcga tggtatcggc gggcacggtg ccaccggccg cagcggtggc
     5761 tagttcgtcg aagcccatgt gggtggcgcg gcccatcatc atcagtccct gcggaaactt
     5821 ggcgcgcgtc gccatcggca ggtggcccat gactttcatg ccggacttgc cggccgcgtc
     5881 ggccagcgcc ttggcatcgt tggcggctac cgcggcgagc acgccctgaa ccgactccat
     5941 gaagccccgc atctcggcga gcacaatggc gcgttcatcg gcggtgagca cgatcgcctg
     6001 ccgtgtctcg ggggccggtt tcgcagcctc gcccatcggc atgccgtggt gcatgtgatt
     6061 gcccatgccg ccgcccatgc ccatctgggc cagtgccgcg gtcgtggcgc cggcaagaag
     6121 cagcgtggta attgctcgtt tcatgatctt ctccccatcg ttgcgcagga ttgcgaccgt
     6181 ctcagtataa gcaatcgcta acgtgcgccg cgtaacctgg gttacgtgcc cgtgttccgg
     6241 cgtcgtttcg gtggacctcg caaggcaccc cctgaactct ttcccgctcg acgctgccag
     6301 acggggcaca aaccaatccg caaccatgaa cgggagccca gcatgatcga cctctacacc
     6361 gccggcacgc ccaacgggca caaggtctcg atcgcactcg aggaactggc gctgccctac
     6421 gaactgcacg tgctcgacct ctcggcacag cagcagaagc agccgtggtt cctggcgatc
     6481 aacccgaacg gccgcattcc ggcgatcgtc gatcgagaca ccgacgactt cgcggtcttc
     6541 gagtcgggcg cgatcctcgt ctacctcgcc gagaagaccg ggcgactgct gccggccgat
     6601 gtgaaggggc gctcgcgggt gctgcagtgg ttgatgttcc agatgggtgg catcgggccg
     6661 atgatggggc aggccaacgt gttctatcgc tacttccccg agaagatcca gccggcgatc
     6721 gaccgctatc agggcgagag caagcgcctg ttccgcgtcc tcgacgggca tctggccgac
     6781 aacgaatacc tcgccggcga ctactcgatc gccgacatcg ccaactgggc ctgggtgcgt
     6841 acgcaccgct ggtccggggt cgagatcgac gacctgccgc atctacgtcg ctgggtcgag
     6901 gcgatccggg cgcggccggc cgtgcagagc ggcatcgatc ggcctcgctc gtcgatcgac
     6961 agccgcctag aagacccggc agaggcggat cgcttcgccg ccgaggcgcg gaagatggtc
     7021 gagaccgggc agtcgaaggc cggcggcgtg tgatcttcat tgggccggga cctcgcccgg
     7081 cggcgtgtgg tcctcattgg accgggacct cgaccgggcg cccggaaatt tgattcagct
     7141 tatggttgcg ctgccgtcga tcgggcagac tgcgccgtct ttcaccgttg cccctcccct
     7201 ggtttccgct catgtccctg ttccgctacc cgatcttctt cgcgccgcac cgtttctttt
     7261 tcgccgccgg cttcgcccag tgggccttcc tgctgatctt ctggacgctg gagatgctcg
     7321 cccgcgtcgg cggcaacgat ctgccgtgga gcggcatcgc gccggcgcgc tggctgcatg
     7381 gggcgctgtt cgtcttcggg ccgtttccct tgtggatctt tgccttcgcc tttacggcga
     7441 tgccgcgctg gctaaagctg ccgccggtct tgccgcgcga gtacctgccg ggcggactgg
     7501 ccatcgccgc cggctggctg ctcgccgacc tagcactttg gggcgggtcg ttgcgcctga
     7561 tgttgatcgg cttcggcacg gtcggcgccg gctggggcct gctgctgatg accttgtggc
     7621 gaattgcccg ccgggcgccg accggcgaca gccatccgcg gctgttggcc ggtgccgtga
     7681 cgctcggcgt gccctcgctc tggctcgccg tctggggcct gtcgacggcc catgccgggc
     7741 gtatcgacct ggcgctgact ctcggcgtct gggccttcct gctgccgacc ttcgtcgtcg
     7801 tcgcgcaccg catgctgcct ttctttaccc gctcggcgct gccgcaggtg gtgccctggc
     7861 agccgcagtg ggtgttgtgg tgcttgcctg cgtgcctgct cgcctacggc ctgtcggtac
     7921 gctacacgcc gacgctggcc tggttgccgg cgttcgcggg attggcgatt tcggccctgg
     7981 tgtcgatgcg ctggcagttg cgcgcctcct tcgccaatcg cctgctggcg acccaccacg
     8041 tggcgctggt ctggctctcg gtggccttcg cgctcggcgc gctgcagtcg gcgctgctcg
     8101 ccgccgacat cctctggggc ggcatcgcgc cgctgcacgc ggtgaccttc ggctttgccg
     8161 tgaccatggt cttcggcatg gcgacgcgcg tgacgctggg ccattcggga cagccgatcg
     8221 gcgcaacgcc gctgctctgg cggctgttct ggaccctgca gctggccgtc gtgctgcgtc
     8281 tggccgccga tctgctgccg cggctggcgg tggtgttgtt gccgctggcg gcacttctca
     8341 gcatcgtcgt attcggtgcg tggctgtggc agtacgccag ctattgggcg atcgacgccg
     8401 acgaataagg cgcgtcagcg ccggtgcgcg agcaggtaga gtgagggaat cagcaggagg
     8461 ctgaccagca tcgagaagga cagcccgaag accatgcaca cggccagcgg gcgaagcatc
     8521 tccgaaccct cgcccaagcc gagcgagagc ggcagcatgc cggcgacggt ggtgaatgtg
     8581 gtcatcagga tcggccgcag ccgcagccgg gcggcctcga cgatggcgtc gagcagcgcc
     8641 tcgccgcggg cgcgcaggat ctctatatat tcgaccagca cgatcgcgtt gttgacgacg
     8701 acgccgatca gcatgatcac gcccagccac accggcatcg acagcggcag gccggtggcg
     8761 agcaggccga tcagcacgcc gattagcgcg aagggcacgc cggcaaggat gatcgccggg
     8821 ttcttcagcg actcgtactg cacggtcatc accacgaaca cgagaaacag cgcgagcgcc
     8881 gcgagtgtgg cgaccagcgt cttgccgcgc tcgagcgttt cgtaggcgcc gccgaaatcg
     8941 agtgagtagc cggccggcag ttcggccttt gccagcgtta cttgcgccgc gcggatcgcg
     9001 gcggcgaggg gcacttcacc agtaagcgcg gcggtgatct cgacgatgcg ccgctggttc
     9061 tcgcggcgga tctcggtcgg cgcgctgatc atgccgatcg aggcgacgtc gccgatggtg
     9121 atcggcgggc gcgcgccacc accgccgacg atgattgccg ccagcgccgc cgggctgtcg
     9181 acgcttcgcg gcggcaggcg gacgcggatg tcgtaggcgc ggtcgccgtc gaggaagtcg
     9241 cccaccacga ggccgtcgag cgcgatcgcc aacgcgcggc cgaccgtttc gacatcgacg
     9301 ccgagttcgg cggcacgcgt gcggtcgacg gttaccgcga attcggggcg gcgctcctcg
     9361 gccgagtggg cgaggttgcg caggcccggc tggccgcgca gcgtcgctac caatcggtct
     9421 gcgatgcgcg ccagttcgtc gagctcgggg ccgagtatgc ggatgctgac ttcctcgtcg
     9481 gagcgcgcca ggcgcacgcc gcggatgccc gagggacgcg cgcgcacggt gacgccggcg
     9541 agttcggcct tggcgacggc gcgcgagaaa tcgccgaccc aggcctggat cgttcggttg
     9601 cgctgtcccg cgggtacgag ctggatcgcc agcgtgctgc ggttcgggcg ttcgcgcgcg
     9661 gtgcggccga aaatcgagcc gccggcgatc accgacagtc cgtcgacctc gccgacttcg
     9721 agcgcgatgg cctcgatgcg ccgcacgtcg cggtccatgt catcgagcga acggccggtg
     9781 tcggtgttga tctgcacctg cacgcggccg tcgtccatgg tcggcaggaa ctcctgctgg
     9841 ccggcgagcg accagaacag gccggcggca ccggcgagca ggacgagggc aagggcggcg
     9901 acagcgagcg gtgcggccag cgtgcgtgtc accagtggcc ggtagattgc ctgcagccgc
     9961 gccaccatcg cctggatcgc ccgcgtcacg cgcgacggct caggcgggcg gctgcgcgcg
    10021 gccagcgccg gcaccagcgt taccgcgacg atcagcgagg cgacgatggc ggcggtaatg
    10081 gtgaagatca gctcgcggaa gagtagcccc acgaggccgc tgatgaacag gaagggcagc
    10141 acggcggcga ggttggtgct ggtggccgcg atgatggcgc tggtgacctc gccggcggcg
    10201 ccggtcgcgg cgaccagcgg cgcctcgccc tggctctggt gccgctcgat gttctcgagc
    10261 atgacgatgg tgttgtcgac gagcattccg acgccgagcg ccagcccacc gagcgtcatc
    10321 aggttcagcg acagcccgcc gagcgccatg atcgcgaagg tgatcatgat cgagatcggg
    10381 attgcgctgc cgatgatcag cgtgccacgc acgctgccga ggaaggcgta gaccacggtc
    10441 atcgccagca ccgcgccggc gagcgccgcg taggtagcgt tggcgagcga ctggcggacg
    10501 tagatcgcct ggtcggatac ctggtcgatg gcaatgtcgg ccggaatcag cgcgcggcga
    10561 tgcaggtcgg caagccgcgc ggcgaccacg tcggccaccg cgaccgtgtt cgcggtgggt
    10621 tgcttctgaa tagcgaggcg gacgcccggc gtgccgttga agcgcacgcg gatccgttcg
    10681 tcctcctgcg cgtcgacgac ctcggcgatt tcggcgagcc gcaggaagct gccgtcggct
    10741 aatcttatcg gcaggccgcc gatctcggcg acgctggcca gccggccggt ggtgcggctg
    10801 gcgatttcct gctcgcccat gtgcaggcgt ccggccggcg cgtcgagatt gccgcgggcg
    10861 atcgcctcgg cgatgtcgtt
//
LOCUS       DATDAA010000137         4002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_324341,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000137 DATDAA010000000
VERSION     DATDAA010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324341"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VIS73_04400"
     CDS             <1..470
                     /locus_tag="VIS73_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762430.1"
                     /translation="RVQGELLPAGQALLQEAALDGHWGLALVSGTPCDSRCGETLDAL
                     RRVHVALYKSMPAVRRLWLADGDDLAVHAEALHARWPDLALVVADPSLRSQLLAPPVA
                     VPTRTLTDNAAIPLAADAVVVFDPAGRAVLRYGSPVDAKAVLTDLRRLLSPSR"
     gene            481..1410
                     /gene="cyoE"
                     /locus_tag="VIS73_04405"
     CDS             481..1410
                     /gene="cyoE"
                     /locus_tag="VIS73_04405"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY5762431.1"
                     /translation="MNPLTATRSLELAAPDWRRRLSAFAALCKPRVNLLIVFTAMIGM
                     VLAPVDISLLRFLAASAGIGLVACGAAAINCLVERTVDARMARTRGRPLPRGELAPAE
                     ALTLAIVLGAAGLALLHAFTTTLATWLTLATFLGYAVIYTAILKPATPMNIVIGGASG
                     AMPPVLGWTAMTGSMAAEPLALFLIVFLWTPPHFWALAMARRDDYARAGFPMLPITHG
                     IGLTCLHSLLYVIMLTAASFLPVTLGMAGGVYALAVAWLNALFLAQAIGLVRKYSDAA
                     SRRMFGFSIAYLSLLFAALFADRLLAPWLPRWF"
     gene            1452..2393
                     /locus_tag="VIS73_04410"
     CDS             1452..2393
                     /locus_tag="VIS73_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY5762432.1"
                     /translation="MRLLAVLLAFVVIGLGAFVRLSDAGLGCPDWPGCYGHWLGVPVT
                     DAETAEAAAAYPGKPIDPNRAWKEMVHRYAAGTLGLLILGLFVAGWRRAAQDGAPWLE
                     STLLGVVVFQALLGMWTVTELLKPLIVTAHLLGGMLTLSLLIALAMRDAGGAQAMTPA
                     LRRAGSMALLAILVQIALGGWVSSNYAALACQGFPACNGALWPVADWEAGFEFWRELG
                     QTATGGTLPSAALVAIHWAHRLGALAVLVLVGNFAWRLYTARCASAAALLAGLLALQL
                     SLGIANVLSHLPLPLAVAHNLGAALLLAACLSLRLRR"
     gene            2431..3279
                     /locus_tag="VIS73_04415"
     CDS             2431..3279
                     /locus_tag="VIS73_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEY5762433.1"
                     /translation="MHSARRAALALAVTLAVAQSPSLRAQTTAEIEDGIADLARRGTE
                     GLPALASHEPVYFVVGRRDDTSARFQFSFRYRMFDEEGIVVRWFRPLAGMHFAYTQTA
                     VWDLSDDSKPFRDTSYKPALLWHWRGGPQIGQRYGWQLDAGFEHESNGRDVDLSRSIN
                     IAFVKPEYRRYIAQRRYAGIGVKAYSYIDREENPDIADYRGHADWVLFLGRDDGWLLK
                     STIRQGAAGHGSVQTELSYPLRRPFFSDTGGYIFVQHFNGYGETLLDYNVRRDAELRF
                     GFAIVR"
     gene            3592..4002
                     /locus_tag="VIS73_04420"
                     /pseudo
     CDS             3592..4002
                     /locus_tag="VIS73_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611549.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          591 a         1417 c         1270 g          724 t
ORIGIN      
        1 tccgtgttca aggcgaattg ctgccagccg gccaagcgct gctgcaggaa gcggcactcg
       61 atggtcactg gggactggcg ctggtcagcg gtacgccttg cgacagtcgc tgcggcgaga
      121 cgctcgatgc gctgcgccgc gtccacgtcg cgctttacaa aagcatgccg gcggtgcgcc
      181 ggctctggct cgccgacggc gacgacctcg cggtccacgc cgaggcgctg cacgcgcgct
      241 ggcccgacct cgcgctggtg gttgctgatc cgtcgctgcg cagccagctg ctcgcgccac
      301 ccgtcgccgt gccgacccgt acgctcaccg acaacgccgc aattccgctt gccgccgacg
      361 ccgttgtcgt ctttgacccg gccggccgag ccgtgctgcg ctacggcagc ccggtcgatg
      421 ccaaggccgt tttgaccgac ctgcgccggc tgctcagccc atcgcgctag gggatagaac
      481 atgaacccgt tgaccgccac ccgcagcctc gaactcgccg cccccgactg gcgccgccgc
      541 ctgtcggcct tcgccgccct gtgcaagccg cgggtgaacc tgctgatcgt gttcaccgcg
      601 atgattggca tggtgctggc cccggtcgac atctcgctgc ttcgctttct cgccgcctcg
      661 gccggcatcg ggctggtcgc ctgcggcgcc gcggcgatca actgcctcgt cgaacgcacc
      721 gtcgatgcgc gcatggcgcg cacccgcggc cggccgctgc cgcgcggcga actggcgccg
      781 gccgaggcgc tgacgctggc gatcgtgctc ggcgccgccg gcctcgcgct gctgcacgcc
      841 ttcaccacga cgctggcgac ctggctgacg ctggcgacct tcctcggcta cgcggtgatc
      901 tacacggcga tcctcaaacc ggcgacgccg atgaacatcg tcatcggcgg tgcctccggc
      961 gcgatgccgc cggtcctcgg ctggaccgcg atgaccggct ctatggcggc cgagccgctg
     1021 gcgctgttcc tgatcgtctt cctgtggacg ccgccgcact tctgggcgct ggcgatggcc
     1081 cgccgcgacg actacgcgcg cgccggcttc ccgatgctgc cgatcaccca cggcatcggc
     1141 ctcacctgcc tgcacagcct gctctacgtg atcatgctca ccgccgcgag cttcctgcca
     1201 gtgacgctgg gcatggccgg tggcgtctac gcgctcgcgg tcgcctggct caacgcgctg
     1261 ttcctcgcgc aggcgatcgg cctggtccgc aagtacagcg acgccgcctc acggcggatg
     1321 ttcggcttct cgatcgccta cctgtcgctg ctgtttgcgg ccctgttcgc cgatcgcctg
     1381 ctcgcgccgt ggctgccacg ctggttctga gccgggccgc agcacctgcc gcaagcaatc
     1441 gcacgcgggc cgtgcgcctg cttgcggtgc tgctcgcctt cgtcgtcatc ggcctcggcg
     1501 catttgtccg gctctccgat gcgggtctcg gctgccccga ctggccggga tgctacggcc
     1561 actggctcgg cgtgccggtg accgacgcgg aaaccgctga ggccgccgcc gcctacccgg
     1621 ggaaacccat cgacccgaat cgcgcctgga aagaaatggt tcaccgctac gccgccggca
     1681 cgctgggcct gctgatcctc ggcctgttcg ttgccggctg gcgccgggcc gcccaagacg
     1741 gcgcaccttg gctggaatcc acgttgctcg gggtggttgt cttccaggcc ctgctcggca
     1801 tgtggaccgt caccgagttg ctcaagccgc tgatcgtcac cgcccacctg cttggtggca
     1861 tgctgacgct gtcgctgctg attgcgctgg cgatgcgcga cgccggcggc gcacaagcga
     1921 tgacgcccgc tttgcgccgc gccgggtcca tggccttgct ggcgatcctg gttcagatcg
     1981 cgctcggggg ttgggtttcc agcaactacg cggcgctcgc ctgccaggga ttcccggcct
     2041 gcaacggtgc gctctggcct gtagccgact gggaagccgg attcgagttc tggcgcgagc
     2101 ttgggcaaac ggccaccggc ggcaccctgc cgagcgccgc gctggtcgcc atacactggg
     2161 cgcaccggct cggcgcgctg gccgtgctgg tgctggtcgg caactttgcc tggcgactct
     2221 acacagcacg ctgcgcgtcg gcagccgcgc tgctcgccgg tctgcttgcc ctgcaactgt
     2281 cgctgggcat cgccaacgtc ctgtcgcact tgccgctgcc gctggcggta gcccacaacc
     2341 tcggcgctgc gctgctgctc gccgcctgtc tgagcttgcg cctgcgccgg tagaatcgcg
     2401 gcttcgccga cccgattcga gccgcgcccc atgcattccg cccgccgcgc cgccctggcg
     2461 ctcgccgtca cgctggcagt cgcccagtcg ccctcgctac gggcgcaaac gactgcggaa
     2521 atcgaggacg gcattgccga cctggcccgc cgcggcaccg agggcctgcc ggcgctggcc
     2581 tcgcacgaac cggtctattt cgtcgtcggg cgccgcgacg acacctcggc gcgcttccag
     2641 ttttctttcc gctaccgcat gttcgacgaa gagggcatcg tcgtccgctg gttccgcccg
     2701 ctcgccggca tgcacttcgc ctacacgcag accgcagtct gggatctctc cgacgattcg
     2761 aagcccttcc gcgacactag ctacaagcca gcgctactct ggcactggcg cggcggaccg
     2821 cagatcggcc agcgctacgg ctggcagctc gacgccggct tcgagcacga atcgaacggc
     2881 cgcgacgtcg acctctcgcg ctcaatcaac atcgccttcg tcaagcccga gtaccgccgc
     2941 tacatcgcgc agcgccgcta cgccggaatc ggcgtcaagg cctacagcta catcgaccgc
     3001 gaagagaacc ccgacatcgc cgactaccgc ggccacgccg actgggtcct cttcctcggc
     3061 cgcgacgacg gctggctgct gaaatcgacg atccgccaag gcgccgcggg ccacggcagc
     3121 gtccagaccg aactctcata cccgctgcgc cggcccttct tctccgacac cggcggctac
     3181 atcttcgtgc agcacttcaa cggctacggc gaaacgcttc tagactacaa cgtgcggcgg
     3241 gatgcggaac tgcggtttgg gtttgcgatt gtgcggtaat tgagatggcg cccgccggag
     3301 gacagcagtc gaccccgaag agacgctcgc tgtaccggag gtccaacggc agggttctga
     3361 gtggagcagt caaacaaagc gatggccggt cgagtgcacc tcggcctttg ctgtctttca
     3421 cgtcgcgcga tttgagtggc cggtgtcagc ctggctgctg accttcagat tcaacggcgg
     3481 ccttcagtca atcctgttta tcaaccacga aaccggtgat gtggtcggct gaaaccgggc
     3541 cagacggaag gttagttttt cggcaaactt tctctaggaa gggagtttgt catgaagaag
     3601 aagcggttct cggttgagca gatcgttgcg attttgaagc aggcggaatt gggcatgccc
     3661 gttgccgacg tggttcgcca agtcggtatt tccgagcaga cgttctaccg ctggaagaag
     3721 ctttatagcg gcctgcaatc agaccaggtg cgcgagttga agcagctgca agatgagaac
     3781 agtcggctca agaagttggt cgccgagttg agtctcgaca aggcggttct gcaggacgtt
     3841 ctgtcaaaaa agtttcccgg cccgcgctga agaggaccgt ggtgacgtat ctcatgagtc
     3901 accacggcta tagcgagcgt cgggcctgcc gactaaccaa acagcatcgc tcggtccaga
     3961 aataccaaag tgtcttaaat ccgcggacgg agcttcggca ac
//
LOCUS       DATDAA010000138         2534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_44764,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000138 DATDAA010000000
VERSION     DATDAA010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44764"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VIS73_04425"
     CDS             complement(<1..836)
                     /locus_tag="VIS73_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762434.1"
                     /translation="MGRDRLAGTVSMTVSLIDLRVALLAALALSTPPAAAQDPAPDTP
                     EHVAGEQAYPFDTRDPRLEEGEAIELPDSVAPKLALLAPEELEFLQGGDARQFTGSLE
                     DTVEALEERSPEEVRAWVGAMRDMLSAIRYVEGRDLPNIPFNTGSPAFNAWRMERPRS
                     MDPPREPGALPLGRYGAYRGYGGPPTFGGFPLALTMEDLLAAEVDVAILGAPLNMGSG
                     WRDSGRRATTDLRIHGWPMRGSDQYALVSAARELNIVDYGDVAVDNDSTERSMQHVRE
                     IVR"
     gene            complement(838..2229)
                     /locus_tag="VIS73_04430"
     CDS             complement(838..2229)
                     /locus_tag="VIS73_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEY5762435.1"
                     /translation="MNSRGRSRRFVLALTGSLALLPGQQALGQWSVPDELQSSLGALT
                     AEQAEFITSGAALEFIPERQLEHELATRDDDGLRRLVDDLKALAAQMAYDPERDMGAA
                     PLNLHTEFFNGPALATPAPLRELERAPRPFSVHRYLFPQSGVPTFGGAKVAVWPEDLV
                     AGRVDVAIIGVPANMSSGRRDSGNGPDAMRGLDTIATPDAQSLLEPFKVLSVVDYGNF
                     AVDNMSTERTVDHVARMVAETAGTGAIPMLVGGDTSMLYPGVKGVAEAHGAGSFGLLH
                     FSAHPDVERFGDHRMSDRQALFRLLDEGIVDGGETVQVGLRGPALTVDTLQWLRDQGV
                     RYHTMAEVRQRGFERVLKRMLREVDRGPDAYFVSVDVSVIDPAEMVAAGRATANGLRL
                     EQVARAVRHVCASKEIVGFEITDLAPMLDFSSLSVMHANAVLNACLAGIAVQKSGLEP
                     GYVHPLALDHGQR"
BASE COUNT          414 a          860 c          833 g          427 t
ORIGIN      
        1 cggacgatct cgcgcacgtg ctgcatggag cgctcggtgc tgtcgttgtc caccgcgacg
       61 tcgccgtagt cgacgatatt cagctcgcgc gccgcgctca ccagcgcgta ctggtccgat
      121 ccgcgcatgg gccagccgtg aatgcggaga tcggtagtgg cccgccgccc ggagtcccgc
      181 cagcccgagc ccatgttgag cggggcgccc aggatggcga cgtcgacttc ggcggcgagc
      241 aggtcctcca tcgtgagcgc cagcggaaag ccgccgaagg tcggcgggcc gccgtagccg
      301 cgataggcgc cgtaacgccc gagcggcaac gcgcccggct cacgcggagg gtccatcgat
      361 cgcggccgct ccattcgcca ggcgttgaac gccggcgacc cggtgttgaa tggaatattg
      421 ggcaggtcgc ggccttcgac gtagcgaatt gccgacaaca tgtcccgcat cgcgccgacc
      481 caggccctga cctcctcagg cgagcgttct tcgagtgctt cgaccgtgtc ctcgagcgag
      541 ccggtgaact gacgtgcgtc gccgccctgc aggaactcga gctcttccgg cgcgaggagc
      601 gccagtttcg gcgccacgct gtccggcagt tctatggctt cgccttcttc cagccgcggg
      661 tcacgggtat cgaagggata agcctgctcg cccgccacgt gctcgggcgt gtcgggcgcc
      721 gggtcctgtg cggcggccgg cggcgtcgac agggccagcg ccgccagcag ggcgacgcga
      781 agatcgatta atgacacggt catcgaaaca gtcccggcaa gcctgtcgcg tcccatgtca
      841 gcgctgcccg tgatcgagcg ccagcggatg cacgtagccg ggttccagac ccgacttttg
      901 caccgcgatg ccggcgaggc aggcattcag cacggcgttc gcgtgcatca ccgacagcga
      961 cgagaagtcg agcatcggcg ccaggtccgt gatctcgaag cccacgatct ccttcgaagc
     1021 gcagacgtgt cgaaccgccc gcgctacctg ctcgaggcgc aggccgttcg ccgtcgcgcg
     1081 cccggccgcg accatctccg ccgggtcgat gacgctgacg tcgacggaga cgaaatacgc
     1141 gtccgggcct cgatcgactt cgcgcagcat ccgtttcagc acgcgctcga agccgcgctg
     1201 ccggacctcg gccatggtgt gataacgaac gccctggtcg cgcagccatt gcaaggtatc
     1261 aacagtcagc gcgggcccgc gcaatccgac ctgcacggtt tcgccgccgt cgacgatgcc
     1321 ctcatcgagc agccggaaca gcgcctgccg gtccgacatg cggtggtcgc cgaagcgctc
     1381 gacgtccgga tgggcgctga agtggagcag cccgaagctg ccggcgccgt gcgcctccgc
     1441 tacgcccttg acccccgggt acagcatcga cgtgtcgccg ccgaccagca tcgggattgc
     1501 gccggtgccc gccgtctcgg ccaccatccg ggcgacgtga tccacggttc tctccgtgct
     1561 catgttgtcg acggcgaaat tgccgtagtc gaccacggac aggaccttga acggctcgag
     1621 cagcgactgt gcgtccgggg tcgcgatcgt gtccagcccg cgcatcgcgt ccggcccgtt
     1681 gcccgaatcg cggcggccgc tgctcatatt ggcgggcacg ccgatgatcg ccacgtcgac
     1741 gcggcccgcg acgaggtcct cgggccagac ggcgactttt gcgccgccga aggtaggaac
     1801 gccggactgc gggaagagat agcggtgcac gctgaacggc ctgggtgcgc gctcgagttc
     1861 cctaagtggc gccggcgtcg ccaacgcggg tccattgaag aattcggtat gcaggttcag
     1921 gggcgccgcc cccatgtcgc gttccgggtc gtaggccatc tgcgccgcaa gggccttgag
     1981 gtcgtccacc aggcgcctga gtccgtcgtc gtcgcgggtg gcgagttcgt gctcgagctg
     2041 tcgttcgggg ataaactcca gcgccgcgcc ggaggtgatg aattcagcct gctcggcagt
     2101 cagcgcgccg agcgaggact gcaattcatc cggcacggac cactgcccaa gcgcctgttg
     2161 accgggcagc agcgccaggc tgcctgtcag cgcaaggacg aaacgccggg aacggcctcg
     2221 agaattcata tgtttttagc tagccacagg gtcgcccagg gattcggcca atcctagtgc
     2281 atttgcggag gactttcccg acaaataagc ggccccggtt ctcgccgcgc agggcaactt
     2341 ttcccgagcg tgattttcca gcgggcaaga aagccccctc agcgctgcgt tttgaaaggc
     2401 gcggcgtgac tccgccacag cccgcccccg gggacagttt ttcgactgtg tcacaaaccg
     2461 cttaatcaga attctttgat ttcatgcgga ttttccatat cgcggactgt gtcacgcccg
     2521 attttcggcg agat
//
LOCUS       DATDAA010000139        27398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_44885,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000139 DATDAA010000000
VERSION     DATDAA010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 27398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27398
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44885"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VIS73_04435"
     CDS             <1..317
                     /locus_tag="VIS73_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5762436.1"
                     /translation="ALVYGAAWASGPAELPANGAGWLAVSAIAVLSTLVAILFFFVGL
                     GRLGAADASTVSTLEPLATIALAAVFLDERLSAMQNGGGAILVAAVILIARARDGCAA
                     RT"
     gene            complement(327..539)
                     /locus_tag="VIS73_04440"
     CDS             complement(327..539)
                     /locus_tag="VIS73_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEY5762437.1"
                     /translation="MTLTDEELARIRNEVHELRAEHRDLDQVIAHLTGNPPADQLLLR
                     RLKKRKLGLKDRIEILEKMLVPDIPA"
     gene            665..982
                     /locus_tag="VIS73_04445"
     CDS             665..982
                     /locus_tag="VIS73_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762438.1"
                     /translation="MFNVKVVFGKGREYDYVFQEADLSGATDIAARQWFDSEFEALEC
                     EPSNPVGKVLIIDKILNVAKYGGEDRFAGQKEWAHRFARNTALALGRDTICVDVAGFS
                     IGY"
     gene            1168..2568
                     /locus_tag="VIS73_04450"
     CDS             1168..2568
                     /locus_tag="VIS73_04450"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY5762439.1"
                     /translation="MISVEKIGGTSMSAFGDVLRHIMMFDPARIYGRIYVVSAYSGVT
                     NQLLEHKKTGEPGIYALFAGGAEYQAALDGLAVSLKKLNAGFAGLGLPLDLADQFVDT
                     RIGEAKKYLEAMHHVLASGYLNRADVLLAAREVLASIGESHSAFNSTEILKANGVRAI
                     FLDLAGFHDDEAWTIDARIHNSFKGLDLANNVVVATGYTKGVEGIMREFDRGYSEVTF
                     SKIAVEVRPAEAVIHKEFHLSSADPNIVGLENAVIVGATNYDVADQLADVGMEAIHPK
                     AAKPMELAGIPIRLKNTFDPQHPGTLVTKDFVGERARVEMVTGSDRVTLVEIHDPSMV
                     GTVGFDLGIMEIFRRHEASYILKATNANSIAHVLWDSAVTPALVDELKASYQVVTVKA
                     SAIVCAIGSNISIPGVLARAAQALADAGVNVNCVSQTLRQVNMQFIVERGDYRKSIIA
                     LNHSLCVTSGAPVPVA"
     gene            2889..4082
                     /locus_tag="VIS73_04455"
     CDS             2889..4082
                     /locus_tag="VIS73_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HEY5762440.1"
                     /translation="MQASSLNLLESDEKVIAASERFGMSRRDFLQFCAAMSATLGLPA
                     GAEAAVAQAVASKQRPSVIWLHFQECTGCTESLLRAEHPTIEKLILDVISLDYHETLM
                     VAAGHQAEAARKAAMKANKGKYVLVIEGAIPTKDGGVYCKVGNQTAIEMTKECAADAA
                     AVIAIGSCASWGGMPSTIPSIPGADSSPTGSAGVAEILGKPVVTIPGCPPNPYNFLST
                     VVHFLTFGTLPAIDEKGRPKFAYSRLIHENCERRAHFDAGRFALEFGDDGHRKGYCLY
                     KLGCKGPETYANCSVALFGDAGAGTWPVACGHPCIGCTEQGIGFTKPIHAIAKLKSVE
                     PPLQYPRIDEPVGKGASLGAAAVLAAVAGAAAGAGAMVAKNLGLSKEAEDAAKKSAQQ
                     PAEKV"
     gene            4085..5110
                     /gene="hybA"
                     /locus_tag="VIS73_04460"
     CDS             4085..5110
                     /gene="hybA"
                     /locus_tag="VIS73_04460"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HEY5762441.1"
                     /translation="MDQTCDSRRKFLKGALAGGAMASAATVPVTVEARGNKTMPPEAL
                     GLLYDATLCIGCKACVAACKRENAVNGATPEFNTGDQDYWDTPLDTTAKTFNVIKMYR
                     NGTMETKDSEKDGYSFMKVSCHHCVDPSCISACPVSAMTKDEKTGIVGYDKDACIGCR
                     YCVAACPFGIPKWDYNNPFGKIGKCELCRHRTWDEKGEPTGSYSACAEVCPTGATLYG
                     KVSDLMAEAKRRMAAKPGSKYVFQRGKIDGQDQRYEGEIGNYVQHIYGEREIGGTQKI
                     TLSAVPFEKVGLPKLPDVSPASRSESIQHALFGNMLALPILTTGLLAWLIKRNSKPEG
                     QSSEGGK"
     gene            5112..6272
                     /gene="hybB"
                     /locus_tag="VIS73_04465"
     CDS             5112..6272
                     /gene="hybB"
                     /locus_tag="VIS73_04465"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HEY5762442.1"
                     /translation="MANHAHPQPAPLGGRLLTPTTMIASVLVFIMLVVLSIRFIFGLG
                     AVTNINDGYPWGIWVAYDVVIGSAFACGGFAVAMLVYIFNKGEYHPMVRPALVASLFG
                     YTLAGVSVLFDLGRYWNVWHMFWPGYSQTNSVMFEVAACISLYILVMWIEFAPTAFEK
                     WGMKDAAKKIEKWMFLVLALGTVLPMLHQSSLGTMLVVFGEQIHPLWQTKMLPLIYLM
                     TAVTLGYAVVIFEACLSSSGFKRPLEMEILRKLAKVMYGILAAYLVIRFGDLVVRGAL
                     GYAFAADFRALAFWVETACFAAPLLLIASAESRDNPKRLFLAAVSLMLAGFLLRINSY
                     LIGYQTGGNFSYFPSVPEMLVTFGIIALEVLLYIIFVRTFPVLPRDVPQGAH"
     gene            6321..8033
                     /locus_tag="VIS73_04470"
     CDS             6321..8033
                     /locus_tag="VIS73_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEY5762443.1"
                     /translation="MSKRVTIDPITRIEGHLRIDVEVDGGKVNKAWSSGQMWRGVENI
                     LIGRDPRDAWAITQRICGVCTTVHAITSVRAVENALNLEVPLNAQYIRNLIILAHAIH
                     DKIVHFYHLSALDWVDVTSALKADPDKTAALAESLSSWGLNGKHEMRRVKEKLASFVG
                     TGQLGIFTNGYWGHPAMKLSPEVNLLACAHYLQALEVQRYANKIVSILGSKTPHIQNV
                     AVGGVANPLSTDSQSVLTIERLLAVKEWIDKLDDFVKNVYLVDLAAIGAFYADWTAYG
                     KGVTNYLAVPDIPLDPKGTQFAIPGGYIPGADLAQFKPIKAFGDPLFKDGVSEAVTHS
                     WYSYADGDGKSLHPFQGETTPKYTDFQDEGKYSWLKSPTFYGKPAQVGPLAQVLCGLA
                     SGHKPTEKYANFVLDTAGSLAKTKIPLSAMHSTIGRHASRAITCAVYLDTIKGQWQAL
                     VDNMAKGDLTTFNKPVFPKGEIQGVGFHEAPRGALSHWVVINDGKIKNYQCVVPSTWN
                     AAPKNEKGEMAPYEASLIGTPIADAEKPLEVLRTVHSFDPCLACAVHIVDEEQKEVVR
                     VKAA"
     gene            8117..8602
                     /locus_tag="VIS73_04475"
     CDS             8117..8602
                     /locus_tag="VIS73_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HEY5762444.1"
                     /translation="MRVVILGIGNILLADEGVGVRAVEAIERDFILPEGVVAIDGGCS
                     AMELLEDLEHLDALIVVDAVYAGQHPGDMVVLEGEEVSKFFRTRMSPHQVGLSDVLAS
                     LEFTGRSPKQLLIVGIKPLSMDLSMDLTPQVAEKLPAMIDRVAAALDGLGCPMRRRLT
                     A"
     gene            8618..8899
                     /locus_tag="VIS73_04480"
     CDS             8618..8899
                     /locus_tag="VIS73_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEY5762445.1"
                     /translation="MCIGIPKKITAIEGAEGEFAWVECADGTGRERVSMMLLEPQPIG
                     AWVLTSLGIAREELTEDEAATINDALAALVDALDGQYDPSQHFADLTRH"
     gene            8909..9514
                     /gene="hybE"
                     /locus_tag="VIS73_04485"
     CDS             8909..9514
                     /gene="hybE"
                     /locus_tag="VIS73_04485"
                     /inference="COORDINATES: protein motif:HMM:NF023365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[NiFe]-hydrogenase assembly chaperone HybE"
                     /protein_id="HEY5762446.1"
                     /translation="MAIERLRKPAIVPRAPEPAQPFKDDPGEIVRAHFQLVWETAMHD
                     MPFVNPALTVEVGGFQRVDGDWIGVVITPWFINLFLLPGGGSLWADWPSGEQRSVELP
                     VGVMDFIADNPGAEAILPAYQYCPLVAPVQSVADQTAARDIAAAAMETVLAPPPATEA
                     TPEGAASEVVVPAPVVTTPASEAPASSPRRGFLRKLAGHRD"
     gene            9558..10121
                     /gene="greB"
                     /locus_tag="VIS73_04490"
     CDS             9558..10121
                     /gene="greB"
                     /locus_tag="VIS73_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEY5762447.1"
                     /translation="MSKAFTRETDGDDEDEELAPALKLPVGTKNYLTPSGYARLSAEL
                     DHLLKRERPKLVEVISWAASNGDRSENGDYIYGKKRLREIDRRIRFLNRRLAQSVVVN
                     PEQPADPDLVFFGATVTIADESGDEQTYSIVGIDEADATRARISWTSPLARALLKARE
                     GDAVRFQSPAGLREVEVVAIRYQAIEA"
     gene            10144..10830
                     /locus_tag="VIS73_04495"
     CDS             10144..10830
                     /locus_tag="VIS73_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5762448.1"
                     /translation="MAKQEQRDPIAVGSGEPPRKRRGLGLGGQMLFGLLVGTAMATGS
                     AADIKRVLADPKSFSASVESGRRLASFCFNCHGEDGHSRIPETPNIAGQNAVYLHEQI
                     RKFANGERRNDFMQGLTRALSEDERINVAIYFAAQKAYPASANAGPVAAAGANLFRRT
                     CANCHGEAGHGKDEIPRVAGQHGIYLTRALSAYRAKDGTRGDPRMSAVAAALSDQEIA
                     SLAAYLSALP"
     gene            10931..12835
                     /gene="htpG"
                     /locus_tag="VIS73_04500"
     CDS             10931..12835
                     /gene="htpG"
                     /locus_tag="VIS73_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEY5762449.1"
                     /translation="MAANETKSGKETLGFQTEVKQLLHLMIHSLYSNKEIFLRELISN
                     ASDACDKLRFEALNNAALYEGVSDLQIRVRFDAEAKTLTVSDNGIGLSREEAIANLGT
                     IAKSGTREFFHSLTGDAKKDAHLIGQFGVGFYASFIVADRVTVVSRRAGLQADQGIRW
                     ESAGAGEFSIESVERPERGTDVTLHLKDSEVELLSSWSLKSIIRKYSDHITLPIRMQK
                     EEWDEEAKKQVLRDEEETVNQASALWARPKSEVDDDAYRAFYKHVAHDYEDPLSWVHA
                     RVEGKQEYTQLLYVPAHAPFDLWDRQARHGIKLYVRRVFIMDDAEQLMPLYLRFVRGV
                     VDSNDLPLNVSREILQQSKDIESIRKGCTAKVLGLLADLAEKDAEKYAKFWGEFGKVL
                     KEGVGEDHANKDKIAGLLRFASTLADTPEEKVSLAEYIGRMKEGQDKIYYVTAETFNA
                     AKNSPHLEIFRKKGIEVLLLADRVDEWVVGHLTEFDGKQLASVAKGGLDLGKLEDAAE
                     KEAAEKEAGELGDVVEKLKKSLAERVKEVRVSHRLTDSASCLIADEHDVTGNLARILK
                     AAGQNAPDVKPILEINPHHPVVLRLKDEATKFDDWAALLFEQALLAEGGQLDDPAGFV
                     RRVNTLRLGS"
     gene            complement(12916..13644)
                     /locus_tag="VIS73_04505"
     CDS             complement(12916..13644)
                     /locus_tag="VIS73_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762450.1"
                     /translation="MNPAMNFRNCSTLALAALLLAACGGPALVQPPAAPAPVTTPEAP
                     PEPVPAAPMPPAEAEPSGPEIGTIRNIPVPEAIPLDLPTVPALPPDELIEPRAGCRLA
                     SDPIACEKRHRASELALAECQGIDPQRKGDCMRDAMVRNEDCSRSRAVSLCEARKYAY
                     DTCRGTYTRERRQCLIDAMPLDDCTKAKVQSAIDRCEAAKKAMEVCRVFDDGIDRRTC
                     LYTELTPFLEEQEKKELEKSQSRN"
     gene            13799..15604
                     /locus_tag="VIS73_04510"
     CDS             13799..15604
                     /locus_tag="VIS73_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY5762451.1"
                     /translation="MSAEPASPQLGTAEHSLQLQEVVEALVSDGLVGKTDADVLVKER
                     RYYQGHDHPLVIIADQKWKSATPPHRTLDLETLTEWLADKVGLEYFHIDPLKIDFSAV
                     TDVMSNAYATRFRILPVLVTTKEVVIATAQPTVREWERELQPILRKEIRRVIANPADI
                     SRFQVEFYNLARSIKKAAESGGAGMSGLSSFEQLVELGKSKQQFDANDQHIVHVVDWL
                     WQYAFEQRASDIHIEPRRELGIVRFRIDGVLHQVYQIPMAVMSAMTSRIKLLGRMDVI
                     EKRRPQDGRIKTRTPAGQEVELRLSTLPTAFGEKLVMRVFDPDVLVRDFTDLGFSPED
                     TGRWREMTKRPNGIVLVTGPTGSGKTTTLYSTLKQLATAEVNVCTIEDPIEMIEPAFN
                     QMQVQGSIDMDFADGVRALMRQDPDIIMIGEIRDLPTAEMAIQAALTGHLVLSTLHTN
                     DAPAAITRLLDLGVPPYLINGTLLGVMAQRLVRTLCPHCRKPNAPRTEDEGMWQALVS
                     PFKAAMPKQVQHSVGCLECRRTGYLGRVGLYEIMVMSPELKKLVTDTTDIAKLREQAY
                     REGMKPLRISGAMKVASGQTTLDEIVRVAPPGDGF"
     gene            complement(15649..17367)
                     /locus_tag="VIS73_04515"
     CDS             complement(15649..17367)
                     /locus_tag="VIS73_04515"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HEY5762452.1"
                     /translation="MSEGAIGLTLGGVGLFLLGMLLMTDGLKAAAGPTLGRVLGSATA
                     TRWRGLFSGIFVTAMVQSSSAVTVAAIGFVNAGLLSFSQSLWVLFGANVGTTMTGWLV
                     ALVGLKIKIEAAALPLIGIGMALRLSGPETRRGAIGGALAGFGVLFLGIGFLQQAFSG
                     AGSAVDLAAFGGLGWRSVIAFVFAGMLLTIVMQSSSASLAVVLTLAETGVLPIGEAAA
                     AVIGANVGTTVTALLAAVGATANARRAAAAHVLFNVVTGLVALLLLPFLVGLVAWLRA
                     LLEMGAAPAVTLALFHTVFNVLGVVLMWPLANRLAIFLQHRFRRREDDLAQPRYLDAN
                     VASVPSLAVDALRREIVRLGGLSLGAVLTRLAQAPGVHGSAAAAAALSHAISGFVTRM
                     HRGVMSAASADALARLLRAQRYYDQCAELAAEADAVATMDGRLDAELRDSVTELTDEA
                     GELLRQLAPDTAPVAPASAHQRERFESHYQPLKAALLAAGARGELEIDAMVALLRRLS
                     SLRRAIDQADKAAHLLAAPQVDAENEAAAEDGADSETHPEESTSPSPDRYGDVDGEFS
                     ADGPAR"
     gene            complement(17508..18173)
                     /locus_tag="VIS73_04520"
     CDS             complement(17508..18173)
                     /locus_tag="VIS73_04520"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY5762453.1"
                     /translation="MKRLIPLAMSLAIATHGALAADMTDEQKTLYALGQMLASQTTVF
                     RLSADEITHVQKGWRDATLKTKSAVDVAQYAPKVNALAQSRAEQTAREESEKGKKLLA
                     TAAAEKGAVKAASGFIYLPLVVGKGAKPAATDTVRVHYRGTLPDGTEFDSSYSRNEPT
                     EFPLNRVIKCWTEGVAMMKVGGKAKLTCPADTAYGNQSPSPLIKPGATLLFEIELLEV
                     KGK"
     gene            complement(18287..19756)
                     /locus_tag="VIS73_04525"
     CDS             complement(18287..19756)
                     /locus_tag="VIS73_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEY5762454.1"
                     /translation="MLWLALWLPDLPLIAHPGRPLPSAVVDRGRLLACDETATAAGLM
                     PGMRAATARGMLPELALLPRNPVAEATVLDELACWAGQYTPDIVLAPPAALLLEIGGC
                     LRLFGGIDAVLAAVGAGCRERDLAPRMAAAPTPQAALWLAQGSESPPSAGVRPPVIPT
                     REALPDMLAPLPAQLLAASEQQAQQLASFGLKTLGEALRLPLAGLARRLGGDCAVRIE
                     QALGERPDLRPRFVFPERFRQRLTLPGIAHEAGALCFAGDRLIGALCGWLVARQQGVL
                     RCTLTLGHEALPGKPRESMITLTLTTPTRTPQRIARVLRELLERTELPAPVETLTLSA
                     EHCEDLAGKSRALFDTAGSEPEGIELLVEQLRARLGESAVHGISPAAGHRPENATRHT
                     AVGASTTTAMPAAPPRPLLLLPQPETLREIDGRPHQQRDPLELLAGPERIESGWWDGG
                     EPGATGDIRRDYFIARNRQDEYLWIYRTAEGWHLHGIFA"
     gene            complement(19689..20594)
                     /locus_tag="VIS73_04530"
     CDS             complement(19689..20594)
                     /locus_tag="VIS73_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS cell division inhibitor"
                     /protein_id="HEY5762455.1"
                     /translation="MSVRALRPLGPPPSPASAETPAGVTPAPTAESASRPSAGPGIPP
                     ITLDALLHRADLWRGAQLAGSTAATIASGFAVLDAELPGGGWPAGALVELMPGSAATY
                     ASQRDPARAGAMRGNGALSLLLPAIARLTRAGRWVALIAPPLPLHAPALLQHGIDLAH
                     LLHVATADAQERNWASERCLRSGAVAATIVWPGMLSFTARKRLQLAAEAGGGSGFLML
                     PAAAADQSSPAPLRLTITAQASGAALHILKRRGPRCDRPIVLPLARPLPARKRNTPAT
                     ESDHALARPLATRPAADRAPRPAAA"
     gene            complement(20604..21422)
                     /locus_tag="VIS73_04535"
     CDS             complement(20604..21422)
                     /locus_tag="VIS73_04535"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEY5762456.1"
                     /translation="MKRKTYNARAAIPADLQAALDSGREEPLTLAEWLAVDYRKLLAA
                     ALPGAGLGQAVDQLIAVADSADGVMARQRAIGAALLPLIRAAAEPQSCYECVAAHASG
                     VVREWAALCWTADPGYDLATRLTGARRFAADPSMNVREIAWSSFRPWLAEDLDNAVAL
                     LAPWTRDADPNIRRCAIEATRPRGVWCVHIDALKQTPQLALPLLEAVRSDTSRYVQLS
                     VANWLNDVSKHQPDWVRQTTARWLAESPTSETRWIVHHALRTLRKQGVENARTR"
     gene            complement(21475..22077)
                     /gene="lexA"
                     /locus_tag="VIS73_04540"
     CDS             complement(21475..22077)
                     /gene="lexA"
                     /locus_tag="VIS73_04540"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY5762457.1"
                     /translation="MVKLTPRQQEILDFIKNTVEMLGAPPTRQEIASAFGFASANAAE
                     DHLKALARKGVIHLEPGSARGIRLVEQLGLPLIGHVAAGSPLLATEHVQGHLQVDPRL
                     FTPKADFLLRVRGQSMIGIGMLDGDLLAVHKTSEARNGQVVVARISDDVTVKRLRRKG
                     HTVELIAENPDFEPIIVDTRREALTIEGIAVGLIRGGQAL"
     gene            22459..23178
                     /locus_tag="VIS73_04545"
     CDS             22459..23178
                     /locus_tag="VIS73_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762458.1"
                     /translation="MCRAFASYLDRLPRIDPALTCDLDGRLAGVGITRPQWVELDPSE
                     YRDVVYAIEMSLAPRSRGGQERLAGPIAEHPPRADGPYTIPFDRWRRNYASNVQAGHL
                     RPRLRMATLQLSPPDPPVTVLAYTPQRDDELACQDPGAVNNMLSRNRLGDHLFPYDPA
                     TRTLRQIVSSEVVVSSRLRYRAVVFDGRAYLIGVGAAAPAGAFTVEFSRVERYGSDGP
                     QDPLYAGYHLCGFSLAGENRQ"
     gene            23335..23526
                     /locus_tag="VIS73_04550"
     CDS             23335..23526
                     /locus_tag="VIS73_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762459.1"
                     /translation="MSILDVPISRCEAMRTMVVTDQTQAQCAREHQCPPGMACPLCGC
                     FSGDDWREVVVAKESSAQS"
     gene            23637..24377
                     /gene="surE"
                     /locus_tag="VIS73_04555"
     CDS             23637..24377
                     /gene="surE"
                     /locus_tag="VIS73_04555"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY5762460.1"
                     /translation="MRILISNDDGYFSPGIEALAAALREVAQVTVVAPERDRSGASNS
                     LTLDRPLMLRKAANGFYFVNGTPTDCVHLAVTGMLSELPDMVVSGVNLGANMGDDTIY
                     SGTVAAATEGFLLGVPSLAVSLNSRNESNLGAAAAVARELIERISQQRLSGPMLLNVN
                     VPDLPREQIKGIVTTRLGRRHKAEPVVKATNPRGETCYWVGAAGPAQDAGDGTDFHAV
                     LSGFVSVTPLQIDLTHNGQMPIVKQWLQ"
     gene            24379..25065
                     /locus_tag="VIS73_04560"
     CDS             24379..25065
                     /locus_tag="VIS73_04560"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY5762461.1"
                     /translation="MVAVRLRGDRGAGMTSERTRARMIDRLRERGIRDEAVLKALAAV
                     PRHVFIDEALAHRAYEDTALPLGCGQTISQPYVVARAISLLRAGRESRLKGGLGKTLE
                     IGAGCGYQAAVLGCLTPDVYAVERIRELIERAKVNLRTIQQFNVRLKFADGNLGVPEA
                     APFDTIISAAASAIVPPVLLQQLNVGGRILLPLGGADQQLVMFEQTEKGLAESRFDAV
                     RFVPLLQGTQ"
     gene            25062..25916
                     /locus_tag="VIS73_04565"
     CDS             25062..25916
                     /locus_tag="VIS73_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY5762462.1"
                     /translation="MTDRRAPRLALPFVLGLCLVLGACASKSPAPIVDRSPTGWMSWP
                     AASAASAAPPPVSTNAIVPVEAPAGVGYHRVAKGDTLYSIALDNGQDYKEVAEWNKLA
                     DPSQINIGQVLRVIPPGAVVAGNGVAVSQPIGSGSAVETAPLDSGRAPAVVAKSAPAA
                     VSAGGRWAWPSSGNLIAGFSGDKNKGIDLAGKPGDPVSAAGDGKVVYSGSGLRGYGNL
                     VIIKHDAEYLTAYAHNRKILVREGQQVKRGEKIAEMGDSDADRVKLHFELRRQGKPVD
                     PLKHLPAR"
     gene            25919..26872
                     /gene="rpoS"
                     /locus_tag="VIS73_04570"
     CDS             25919..26872
                     /gene="rpoS"
                     /locus_tag="VIS73_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HEY5762463.1"
                     /translation="MVSGQDSEEEGVVEIDAAVDTEGGADELTIEPRAAAAGESDLIN
                     DVTQLYLNEIGSRPLLTPAEEFDFATKARAGDFEARQKMIEHNLRLVVNIAKHYTNRG
                     IPLLDLIEEGNLGLIHAIEKFEPERGFRFSTYATWWIRQSVERAIMNQSRTIRLPVHV
                     VKEINVVLRAIRHLEAANSRETGVEEIAHLIDKSVDEVRHILQLNEHIASLDAPLDID
                     PSHNIGELLADEQSRDPEAELAGHELGALVHQWVDQLEPKHRLVVERRYGLNGHEIAT
                     LESIASELGLTRERVRQIQLEALQKLRRIIRRGGVSRESLL"
     gene            complement(26832..>27398)
                     /locus_tag="VIS73_04575"
     CDS             complement(26832..>27398)
                     /locus_tag="VIS73_04575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY5762464.1"
                     /translation="TYFKRELAAFLLHAREQQLPPESFKSSYAGATGIPQFMPTSIRS
                     YAIDFDGDRRIDLANSPRDAIGSVAAFLQIHGWKPGGRIAHPAKFDSPPPSHWLVAGI
                     LPSLSGPMSLWTTLSDRAPEEVSLIELRSDDAVEYWWGFYNLYVISRYNRSSSYAMAV
                     NLLGQAVADARSEQTARADSPATPRRGG"
BASE COUNT         4713 a         8771 c         9267 g         4647 t
ORIGIN      
        1 ccgcgctcgt ctatggcgca gcctgggcca gcggccctgc cgagttgccg gcgaatggcg
       61 ctgggtggtt ggccgtgtcg gcgatcgcag tcctgtcgac gctggtggcg atcctgtttt
      121 tctttgtcgg ccttggtcgg cttggggcgg ccgatgcctc gacggtctcg acgcttgagc
      181 cgctggcaac gatcgcactg gcggcggtgt ttctcgacga gcgtctgtcg gcgatgcaga
      241 acggcggcgg cgcgatcctt gttgctgcgg tgatcctgat tgcgcgggcg cgtgatgggt
      301 gcgccgcgcg cacctgatgc ggctgattag gcaggaatgt cggggaccag catcttttcg
      361 aggatttcga tccgatcctt tagtcccagt ttgcgtttct tcaggcgacg caacagcagc
      421 tggtctgcag gggggttgcc ggtgaggtgc gcgatgacct ggtcgaggtc gcggtgctcg
      481 gctcgtagtt cgtgcacctc gttgcgaatc cgggcgagtt cttcatcggt cagcgtcatc
      541 ggggtcctca tgcaggcgcc gatggtagga tgcgccgacg caaaactcaa ctcggcgcgg
      601 tgcgttttcc gaatagaatc cgcagtgtcg attggcagaa aaaacaacag aggagaagag
      661 gcccgtgttt aacgtcaagg tggtattcgg aaaagggcgc gagtacgact acgtgttcca
      721 ggaggcggac ctgtcgggtg cgaccgacat cgccgcgcgg cagtggtttg acagcgagtt
      781 cgaggcgctt gaatgcgaac cgtccaaccc tgtcggcaag gtgttgatca tcgacaagat
      841 tctcaacgtc gcgaaatacg gcggcgagga ccgctttgcc ggccagaagg agtgggcaca
      901 tcgcttcgcg cgcaatacgg cgctggccct ggggcgcgac acgatctgtg tcgacgtggc
      961 gggtttttcg atcggatact gatcgccgct tgccgcccgt ccgggcttcg cgttaccgcc
     1021 aacggccgga atccacggcc gtttgtattt tggggggcgt ccaccagccg cattaaagag
     1081 aaaaggccgg gcgtgcgcta aaattcgccg ctttgattcg cgggcgcctg ccgaggcaac
     1141 tcccgcaact taggattcga ggggatcatg atttcggtcg agaaaatcgg cggtacgtcg
     1201 atgtccgcat ttggcgacgt gctgcgccac atcatgatgt tcgacccggc gcggatttac
     1261 gggcgtatct atgtcgtgtc ggcgtactcc ggcgtgacta atcagttgct ggaacacaag
     1321 aagactggcg agccgggcat ctatgccttg ttcgccggcg gcgccgagta tcaggccgcg
     1381 ctcgacggac tcgcggtcag cctgaaaaag ctcaatgccg gcttcgccgg gctgggcctg
     1441 ccactcgatc tcgctgacca gttcgtcgat acgcggattg gtgaggcgaa aaaatacctc
     1501 gaggcaatgc atcacgtgct cgccagcggc tatctcaacc gcgccgatgt gttgctggcc
     1561 gcgcgtgagg tgctggcttc cataggcgaa tcgcacagcg ccttcaattc gaccgagatt
     1621 cttaaggcca acggcgtccg ggcgatcttt ctcgatctgg ccggcttcca cgacgacgag
     1681 gcgtggacca tcgacgcgcg tatccacaac agcttcaagg gccttgatct ggcgaacaac
     1741 gtcgttgtcg ccaccggtta caccaagggt gtcgaaggca tcatgcgcga gttcgaccgt
     1801 ggctactcgg aggtgacctt cagcaagatc gccgtcgagg tgcgtcccgc cgaggcggtg
     1861 attcacaagg agttccacct ttcctcggcc gaccccaaca tcgtcggcct cgagaacgcg
     1921 gtgatcgtcg gtgcgaccaa ctacgatgtt gccgaccagc tggccgacgt cggcatggag
     1981 gcgatccatc cgaaagcggc gaagccgatg gagcttgccg ggattccgat ccgcctgaag
     2041 aacacctttg acccgcagca tccgggaacg ctggtgacca aggacttcgt cggcgaacgg
     2101 gcgcgggtcg agatggtgac aggcagcgac cgggtgacgc tggtcgagat ccacgatccg
     2161 agcatggtcg gcacggtcgg cttcgacctc gggatcatgg agatcttccg ccgccacgag
     2221 gcctcgtaca tcctcaaggc gaccaacgcc aactcgattg cgcacgtctt gtgggatagc
     2281 gccgtgacgc cggcgctggt cgacgaactc aaggcgagct accaggtggt gacggtcaag
     2341 gcgagcgcga tcgtttgtgc gatcggttcc aacatcagta tcccgggtgt tctcgcccgc
     2401 gccgcgcagg cgctcgccga cgccggcgtc aacgtgaact gcgtgtcgca gacgctgcgc
     2461 caggtgaaca tgcagttcat cgtcgagcgt ggcgactacc ggaagtcgat catcgccctc
     2521 aaccactcgc tgtgcgtgac ctcgggtgcg ccggtgccgg tcgcctgacc ggctgtggcc
     2581 gattggcagt gaagaggggc gccgcgtggc gcccctcttg cattcgcgcg ccggccgccc
     2641 gcccgaaggg gtttgtcgcc ccaggtggtt agtagatttc cactttcagc tttccagtgg
     2701 acggcacctc tccacttgtg agttgaaatg ctgcgtcgca ggatagccaa gctaatgttt
     2761 tgcattagaa aagttatctg gcacggaact tgatcctgcc tttcccgcgt gttgaatcag
     2821 aaggcgcccg aaccgggctg tcacagacga gccgaatgac gattcggcca tcacattggg
     2881 gtgaggctat gcaagcgagc agtttgaacc tgctggaatc ggacgaaaaa gtcattgccg
     2941 cgagcgagcg gttcggaatg tcgcgccgcg actttctgca gttctgtgcc gcgatgtcgg
     3001 caacgctggg cctgccggcc ggggccgaag cagccgtcgc gcaggcggtg gcaagcaagc
     3061 agcggccgtc ggtcatctgg ctgcatttcc aggagtgcac gggctgcacc gaatccctgc
     3121 tgcgcgccga acacccgacc attgagaagc tgattctcga cgtcatctct ctcgactatc
     3181 acgagacgct gatggtggcc gccgggcacc aggccgaagc ggcccgcaag gcggcgatga
     3241 aggccaacaa gggcaagtac gtgctggtca tcgaaggggc gatcccgacc aaggacggcg
     3301 gcgtctactg caaggtcggc aatcagaccg caatcgagat gaccaaggaa tgtgcggccg
     3361 atgccgcggc agttatcgcc atcggctcct gcgcctcgtg gggcgggatg ccatcgacga
     3421 ttccgtcgat tccgggtgcc gattcgagcc cgaccggttc ggccggagtc gccgagatcc
     3481 tgggcaagcc ggtggtgacg attcccggct gcccgcccaa cccatacaac ttcctttcga
     3541 cggtggtgca tttcctcacc ttcggcacgc tgccggccat cgacgagaag ggtcgcccca
     3601 agttcgccta ctcgcgcctg attcacgaga actgcgagcg tcgcgcgcac ttcgatgccg
     3661 gtcgttttgc gctcgagttc ggcgacgacg gccatcgcaa gggctactgc ctctacaagc
     3721 tcggctgcaa gggtcccgag acctacgcca actgctcggt ggcgctgttc ggtgatgccg
     3781 gtgctggtac ttggccggtc gcctgcggcc acccctgcat cggctgtacc gagcagggga
     3841 tcggcttcac gaagccgatc cacgccatcg ccaagctgaa gagcgtcgag ccaccgctgc
     3901 agtacccgcg gatcgacgag ccggtcggca agggcgcgtc gctcggcgca gcagccgtac
     3961 tggccgcggt tgccggtgct gccgccggcg ccggtgcgat ggtcgcgaag aatctcgggc
     4021 tctccaagga ggccgaagac gcggcgaaga agtccgctca gcaacctgcc gagaaggttt
     4081 aaccatggac caaacctgcg attcccgccg caagttcctg aaaggggctt tggcgggtgg
     4141 cgcgatggcc agtgctgcga ccgtcccggt gacggtcgag gcacgtggca acaagaccat
     4201 gccgcccgag gcgcttggac tcttgtacga cgccaccctg tgcatcggct gcaaggcctg
     4261 tgttgccgcg tgcaagcgcg aaaacgccgt caatggcgcc acgcccgaat tcaacaccgg
     4321 cgaccaggac tactgggata cgccgctcga cacgacggcg aagaccttta acgtcatcaa
     4381 gatgtaccgc aacggcacga tggaaaccaa ggatagcgag aaggacggct attcgttcat
     4441 gaaggtctcc tgccatcact gcgtcgaccc ctcgtgcatc tcggcctgcc cggtctcggc
     4501 gatgaccaag gacgagaaga ccggcatcgt cggctacgac aaggatgcct gcatcggctg
     4561 tcgctactgc gtcgccgcct gcccgtttgg cattccgaag tgggactaca acaatccctt
     4621 cggcaagatc ggcaagtgcg agttgtgtcg ccaccgcact tgggacgaga agggtgagcc
     4681 gaccggcagt tactcggcct gcgccgaggt ctgcccgacc ggcgcgacgc tctacggcaa
     4741 ggtctccgac ctgatggccg aggcgaaacg gcggatggcc gccaagcccg gcagcaagta
     4801 cgtattccag cgcggcaaga tcgacggtca ggaccagcgc tacgaaggcg aaatcggtaa
     4861 ctacgtgcag cacatctacg gcgagcgcga aattggcggc acgcagaaga tcacgctgtc
     4921 ggccgtgccc ttcgagaagg tcgggctacc gaaactcccc gacgtttcgc cggcgtcgcg
     4981 ttcggaaagt atccagcatg cgctgttcgg caacatgctg gcgctgccga ttctcaccac
     5041 cggcctgctc gcctggctga tcaagcgcaa ttcgaagccc gaagggcagt cgtcggaagg
     5101 ggggaaatag catggccaat cacgcacatc ctcaaccggc tccgctcggc ggccggctgc
     5161 tgacgccgac cacgatgatc gccagtgtgc tggtgttcat catgctggtc gtcctttcga
     5221 tccgcttcat cttcggtctc ggcgccgtca ccaacatcaa cgatggttac ccttggggca
     5281 tctgggtcgc ctacgacgtg gttatcggtt cggcgttcgc ctgcggtggc ttcgcggtag
     5341 ccatgctggt ctacatcttt aacaagggcg agtaccaccc catggtgcgg ccggcgttgg
     5401 tggcgagcct cttcggctat acgctggctg gtgtcagcgt actgttcgac ctcggtcgct
     5461 actggaacgt ctggcacatg ttctggcccg gttactcgca gaccaactcg gtgatgttcg
     5521 aggtcgcggc ctgcatctcg ctctacatcc tggtgatgtg gatcgagttt gcaccgactg
     5581 ccttcgagaa gtggggcatg aaggatgccg cgaagaagat cgagaaatgg atgttcctcg
     5641 tgctcgcgct cggcaccgtg ctgccgatgc tgcaccagag ttcgttgggt accatgctgg
     5701 tcgtcttcgg tgagcagatc catccgctat ggcagaccaa gatgctgccg ctcatctacc
     5761 tgatgacggc ggtgacgctc ggctacgccg tggtgatctt cgaggcgtgc ctgtcctcgt
     5821 cgggattcaa gcggccactc gagatggaaa tcctgcgcaa actggccaag gtgatgtacg
     5881 gcattctcgc cgcctacctc gtcattcgct ttggcgacct ggtagtgcgt ggcgcgctcg
     5941 gctacgcttt tgccgccgac ttccgcgcgc tggcgttctg ggtcgagacg gcctgcttcg
     6001 cggcgccgct gctgctgatc gccagtgcgg aaagccgcga caatccgaag cgtctgtttc
     6061 tggcggcggt gtcgctgatg ctcgccggct tcctgctgcg catcaactcc tacctgatcg
     6121 gatatcagac cggcggcaac ttcagctatt tcccctcggt tcccgagatg ttggtgacct
     6181 tcggcatcat cgcgctcgag gtgctgctct acatcatctt cgtgcgcacc ttcccggtgt
     6241 tgccgcgtga cgtgccgcaa ggcgcccact gaacgattac gcaagcccaa ccattgcaga
     6301 aagattgaac ggagactgaa atgtccaagc gagtaacgat tgacccgatc acccgtatcg
     6361 aagggcacct gcgtatcgac gtcgaggttg acggcggcaa ggtcaacaag gcctggtcct
     6421 cgggccagat gtggcgcggc gtcgagaaca tcctcatcgg ccgcgacccc cgcgacgcct
     6481 gggcgatcac ccagcgcatc tgcggcgttt gcaccacggt gcatgcaatt acctcggtgc
     6541 gcgcggtcga gaatgcgcta aacctcgaag tgcccttgaa cgcgcaatac atccgcaacc
     6601 tgatcatcct ggcgcatgcg atccacgaca agatcgttca cttctatcac ctgtcggcac
     6661 ttgactgggt tgatgtgacg tccgccctta aggcagatcc cgacaagaca gcggcgctgg
     6721 ccgaaagcct ctcgtcgtgg gggctcaacg gcaagcacga gatgcggcgg gtcaaggaga
     6781 agctagcgtc cttcgtcggc accggacaac tcggtatctt caccaacggc tactggggcc
     6841 acccggcgat gaagctctcg ccggaagtaa acctgctcgc ctgcgcgcac tacctgcagg
     6901 cgctggaggt ccagcgctac gccaacaaga tcgtttcgat cctcggctcc aagacgccgc
     6961 acatccagaa cgtcgccgtc ggtggcgtcg ccaacccgct gtccaccgat tcgcagtcgg
     7021 tgctgacgat tgagcgcctg ctcgcggtca aggagtggat cgacaagctc gacgatttcg
     7081 tcaagaacgt ctacctcgtc gacctcgcag cgatcggtgc cttctatgcc gactggacgg
     7141 cctacggcaa gggcgtcacc aactacctgg ccgtgcccga cattccgctc gaccccaagg
     7201 gtactcagtt cgccataccc ggtggctata ttcccggtgc cgacctcgcg cagttcaagc
     7261 cgatcaaggc cttcggcgac ccgctgttca aggacggcgt ctcggaagcg gtcacccact
     7321 cgtggtactc gtacgccgac ggcgacggca agtcgctgca tcccttccag ggcgagacga
     7381 cgccgaagta caccgacttc caggacgagg gcaagtactc gtggctgaag tcgccgacct
     7441 tctacggcaa gccggcgcag gtcggcccgc tggcccaggt gctgtgcggt ctcgcctcgg
     7501 gccacaagcc gaccgagaag tacgccaact tcgtgctcga caccgccggt tcgctggcca
     7561 agacgaagat cccgctgtcg gcgatgcact cgacgatcgg ccgccacgcc tcgcgcgcaa
     7621 tcacctgtgc cgtctacctc gacacgatca agggccagtg gcaggcgttg gtcgacaaca
     7681 tggccaaggg cgacctgacg accttcaaca agccggtgtt cccgaagggc gagatccagg
     7741 gtgtgggctt ccacgaagcg ccgcgcggcg cgctgtcgca ctgggtcgtc atcaacgacg
     7801 gcaagatcaa gaactaccag tgtgtcgtgc cctcgacctg gaacgctgcg ccgaagaacg
     7861 agaagggcga gatggcgccc tacgaggcgt cgctgatcgg cacgccgatc gccgacgccg
     7921 agaagccgct cgaagtcctg cgcacggtgc actccttcga tccctgcctg gcctgtgccg
     7981 tgcatatcgt cgacgaggag cagaaggaag tcgtccgcgt caaagcggcc tgatttcctg
     8041 cgggcggcgc ccggcggagg aggcgtatcc ttccgtccgt gcgccgcttt ttcatttcgg
     8101 ctttccgagg tcttgcatgc gtgtggtgat tctgggcatc ggcaacatcc tgctcgctga
     8161 cgaaggtgtc ggcgtccgtg cggtcgaggc gatcgagcgc gatttcatcc tgcccgaggg
     8221 cgtcgtcgcg atcgacggcg gttgctcggc aatggaactg ctcgaagacc tcgagcacct
     8281 cgacgcgctg atcgtcgtcg atgccgtcta cgcggggcaa cacccaggcg acatggtggt
     8341 gctcgaaggc gaggaggtgt cgaagttctt ccgcacgcgg atgtcgccgc atcaggtagg
     8401 gctctccgac gtgctggcct cgctcgagtt caccgggcgt tcgcccaagc aactgctgat
     8461 cgtcggcatc aagccgctgt cgatggattt gtcgatggac ctcacgccgc aggttgccga
     8521 gaagttgcca gcgatgatcg accgcgttgc tgcggcgctc gatggcctcg gctgcccgat
     8581 gcgccgccgg ctcactgcct gagccgcgtc cgccgccatg tgcatcggca ttcccaagaa
     8641 gatcaccgcg atcgaaggcg ccgaaggcga gttcgcctgg gtcgagtgcg ccgacggaac
     8701 cgggcgtgag cgcgtcagca tgatgctgct cgagccgcag ccgattggcg cctgggtgct
     8761 cacctcgctg ggcatcgccc gcgaggagct gaccgaggac gaagcggcga cgatcaatga
     8821 cgcgctcgca gcactcgtcg atgcgctcga tggccagtac gacccgtcac agcatttcgc
     8881 tgacctgacg cgacactgag gcaaggccat ggccattgag cgcctgcgga aacccgcgat
     8941 cgttcccagg gcacccgagc cggcgcagcc attcaaagac gatccgggcg agatcgtccg
     9001 cgcgcatttc cagctggtat gggaaaccgc gatgcatgac atgcccttcg tcaatccggc
     9061 cctgacggtt gaggttggag ggttccagcg cgtcgatggc gactggatcg gcgtcgtcat
     9121 taccccctgg ttcatcaacc tgttcctgct gcccggcggc ggcagcctgt gggcagactg
     9181 gccaagcggc gagcagcgtt cggtagagct gccggtcggt gtcatggact tcatcgccga
     9241 caatccgggc gccgaagcga tactgccggc gtaccagtac tgtccgctgg tggcaccggt
     9301 gcagtcggtt gccgatcaga ccgccgcacg agacatcgcg gcggcggcga tggaaaccgt
     9361 gctcgcaccg ccaccggcga ccgaagcaac gccagagggt gccgccagcg aggtggtcgt
     9421 gccggcgccg gtggtgacca cgccggccag tgaagcgccg gcatcgtcac cgcgtcgcgg
     9481 cttcctgcgc aagctagccg gccatcgcga ctgagcgttg tgctctagaa tcgcgccctc
     9541 atgaacggtg gcgcacgatg agcaaggctt ttacccggga aaccgacggc gacgacgagg
     9601 acgaagagct cgccccagcg ctcaagctgc cggtcggtac caagaactac ctgaccccga
     9661 gcggctatgc acggctgtcc gcggagcttg accacctcct caagcgcgaa cggcctaagc
     9721 tcgtcgaggt gatctcgtgg gcagcatcga acggcgaccg ctcggagaac ggcgactaca
     9781 tctacggcaa gaagcggttg cgcgagatcg accgccgcat ccgcttcctc aaccgccggc
     9841 tggcgcaatc ggtggtggtc aatccggagc agccggccga tcctgatctg gtctttttcg
     9901 gggcgacagt caccatcgcc gacgaaagtg gcgacgaaca gacttacagc atcgtcggca
     9961 tcgacgaagc cgatgcgaca cgggcgcgca tcagctggac ttcgccgctt gcccgggcct
    10021 tgctgaaggc gcgcgaaggg gatgccgtgc gtttccagag tccggcgggg ctgcgcgagg
    10081 tcgaggtggt ggcgatccgt taccaggcaa tcgaagcttg aaacgctcat tcagggagaa
    10141 attatggcga aacaagaaca acgcgacccg atcgcggtag ggagcgggga gcccccgaga
    10201 aagcgccgcg gcttggggct gggcgggcaa atgctgttcg gcttgcttgt cggcacggcg
    10261 atggcgaccg gcagcgctgc cgacatcaag cgggtgctgg ccgatccgaa gagcttttcg
    10321 gcttcggtcg aatccgggcg gcggctggcc tccttctgct tcaactgcca cggcgaggac
    10381 ggtcacagcc gaattcccga gacgcccaac attgcggggc agaacgccgt ctacctgcat
    10441 gagcagatcc gcaagttcgc caacggcgag cgccgaaacg acttcatgca gggactgact
    10501 cgggccttga gcgaagacga gcggatcaac gtcgcgattt actttgcggc gcaaaaagcc
    10561 tacccggcaa gcgccaacgc tggacccgtg gcggctgccg gcgccaacct gttccgtcgc
    10621 acctgtgcca actgccacgg ggaagcgggg catggcaagg acgaaatccc gcgcgtcgcc
    10681 ggccagcacg gcatctacct gactagggcg ctgtcggcct atcgggccaa ggatggtacg
    10741 cgcggcgatc cgcgcatgag cgccgtggcg gcggcgctaa gcgaccagga gatagcctcc
    10801 ctcgctgcct atctcagcgc gttgccctga gcgccgactg gcggccatcg ggtgccgcgg
    10861 tggcccgcgc ttgaaatcgg gcgcgccgtc cccatctggg cgattcgtct gaatcctccg
    10921 gagaaacgcg atggccgcga acgaaacgaa gtcgggcaag gagacgctcg gcttccagac
    10981 cgaggtgaag cagctgcttc acctgatgat ccactcgctg tacagcaaca aggagatctt
    11041 cctgcgggag ctgatctcca atgcctccga tgcctgcgac aagctgcgct tcgaggcgct
    11101 caacaacgct gcgctctacg agggcgtgtc cgatctgcag attcgcgtcc gcttcgacgc
    11161 cgaggcgaag acgctgaccg tctcggacaa cggtatcggc ctgtcgcgcg aggaggcgat
    11221 cgccaacctc ggcacgatcg ccaagtcggg tacccgcgag ttcttccata gcctgaccgg
    11281 cgacgcgaag aaggatgctc acctgatcgg ccagttcggc gttggcttct acgcctcctt
    11341 catcgtcgcc gatcgcgtta ccgtcgtctc gcgccgcgcc ggcctgcagg ccgaccaggg
    11401 catccgctgg gaatcggccg gtgccggtga gttctcgatc gagtcggtcg agcgtcccga
    11461 gcgtggcacc gacgtgaccc tccatctcaa ggacagcgaa gtcgaattgc tctcgtcgtg
    11521 gtcgctgaag tcaattatcc gcaagtactc agaccacatc actctgccga tccgcatgca
    11581 gaaggaagag tgggacgagg aggcgaagaa gcaggtgctg cgcgacgagg aagagaccgt
    11641 caaccaagcc tcggcgctgt gggcgcgacc caagtcggaa gtcgacgacg atgcctaccg
    11701 cgctttctac aagcacgtcg cgcacgacta cgaggacccg ctctcttggg tgcacgcgag
    11761 ggtcgagggc aagcaggagt acacccagct gctctacgtc cccgcccatg cgccctttga
    11821 cctctgggac cggcaggcgc gccatggcat caagctttac gtgcgccgcg tcttcatcat
    11881 ggacgacgcc gagcagctga tgccgctcta cctgcgcttc gtgcgcggtg tcgtcgattc
    11941 gaacgacctg ccgctcaacg tctcgcgcga gatcctgcag cagagcaagg acatcgagtc
    12001 gatccgcaag ggctgcacgg ccaaggtgct cggcctgctc gccgacctgg ccgagaagga
    12061 cgccgagaaa tacgcgaagt tctggggcga gttcggcaag gtgctcaagg aaggcgtcgg
    12121 cgaagaccac gccaataagg acaagatcgc cggtctgctg cgctttgctt cgacgctggc
    12181 cgatacgccc gaggagaagg tgtcgctagc cgaatacatc ggccgcatga aggaagggca
    12241 ggacaagatc tactacgtca ctgccgaaac cttcaacgcc gcgaagaaca gtccgcacct
    12301 tgagatcttc cgcaagaagg gcatcgaggt gctgctgctc gccgaccgcg tcgacgagtg
    12361 ggtggtcggc cacctgaccg agttcgacgg caagcaactc gcctcggtgg ctaagggcgg
    12421 gctcgatctc ggcaagctag aggacgccgc cgagaaggaa gccgccgaga aggaagccgg
    12481 cgaactgggc gacgtggtgg agaagctcaa gaagtcactc gccgagcggg tcaaggaagt
    12541 ccgcgtctcg catcgcctga cggactcggc ctcgtgcctg atcgccgacg agcacgacgt
    12601 taccggcaat ctggcgcgca tcctcaaggc ggccgggcag aacgcgccgg acgtcaagcc
    12661 tatcctcgag atcaacccgc atcacccggt cgttctgcgc ctgaaggacg aggcaacaaa
    12721 gttcgacgac tgggccgcgc tgctcttcga acaggcgttg ctagccgagg gcggccagct
    12781 cgacgatccg gccggtttcg tccgtcgagt caacacgctg aggcttggta gctgaggcag
    12841 cgatgcaaca aaaaacccgc gttttcgcgg gtttttttgt gcccgccgcc aacgctggcc
    12901 gggcaggccg ggcgctcagt tgcggctctg gctcttctcc agttccttct tttcctgctc
    12961 ctcgaggaaa ggcgtcagct cggtgtagag gcaggtacgg cggtcgatgc cgtcgtcgaa
    13021 cacgcggcag acttccattg ccttcttcgc cgcctcgcag cggtcgatgg cgctctgcac
    13081 cttggccttg gtgcagtcat cgagcggcat tgcgtcgatc aggcattgcc ggcgttcgcg
    13141 cgtataggtg ccgcgacagg tgtcataggc gtacttgcgc gcttcgcaca gcgacacggc
    13201 gcgcgagcgc gagcagtcct cgttgcggac catcgcgtcg cgcatgcagt cgcctttgcg
    13261 ctgcgggtct atgccctggc attcggcgag cgctagttcg ctggcccgat ggcgcttttc
    13321 gcaggcgatc gggtcggagg cgagccggca gccggcgcgc ggctcgatca gctcgtcggg
    13381 tggcagggcc ggcacggtcg gcaggtcgag cgggatcgcc tcggggaccg gaatgttgcg
    13441 gattgtgccg atctcggggc cgctcggctc ggcttcggcc ggcggcatcg gggcggccgg
    13501 tacgggttcg gggggcgcct ccggcgttgt gaccggcgcc ggcgcggccg gtggctggac
    13561 cagcgccggg ccgccacagg cagcgagcag cagtgctgcc agcgcgagcg tcgagcaatt
    13621 tctgaaattc atggcgggat tcatgccaac gtccttgtgg tgcggatttg cgaggcgtaa
    13681 ctctgtcaca aatcggccgg caggcacagg gccgttgcca catcggcggc cggaccgggt
    13741 ttgggcagcc gttcgcggct cggctatagt ttcgcattgc cacgccatgg atctccgcat
    13801 gtccgctgaa cccgccagcc cgcagcttgg taccgccgaa cactcactgc agttgcagga
    13861 ggttgtcgaa gcgctggtca gcgacggcct ggtgggcaag accgacgccg atgtcctggt
    13921 caaggagcgc cgctactacc aggggcacga tcacccgctg gtgatcatcg ccgaccagaa
    13981 gtggaaatcg gcaacaccgc cgcatcgcac gctcgacctt gaaacgctga ccgaatggct
    14041 cgccgacaag gtcgggctcg agtacttcca catcgacccg ctgaagatcg acttctcggc
    14101 cgttaccgat gtgatgtcga atgcctatgc cacgcgtttc cgcatcctgc cggtcctggt
    14161 tacgaccaag gaggtggtga tcgccaccgc gcagccgacg gtccgcgagt gggagcgcga
    14221 gctgcagccg atcctgcgca aggagatccg ccgcgtcatc gccaacccgg ccgacatcag
    14281 ccgcttccag gtcgagttct acaacctcgc gcgttcgatc aagaaggccg ccgagagcgg
    14341 tggcgccggc atgtcggggc tgtcctcctt cgagcagctg gtcgagctcg gcaagtcgaa
    14401 gcagcaattc gatgccaacg accagcacat cgtccatgtc gtcgactggc tctggcagta
    14461 cgccttcgaa cagcgcgcct ccgacatcca catcgagccg aggcgcgaac tgggcatcgt
    14521 ccgcttccgc atcgatggcg tgctgcacca ggtctaccag attccgatgg cagtgatgag
    14581 cgcgatgacc agccgcatca agctgctcgg acgcatggac gtgatcgaga agcggcgccc
    14641 gcaggatggc cggatcaaga cgcggacgcc ggccgggcaa gaggtcgagt tgcgtctgtc
    14701 gacgctgccg acggccttcg gcgagaagct ggtgatgcgc gtcttcgatc ccgacgtgct
    14761 ggtccgcgat ttcaccgatc tcgggttctc gcccgaggac accggccgct ggcgcgaaat
    14821 gaccaagcgc cccaacggta tcgtcctcgt caccgggccg acaggctcgg gcaagacgac
    14881 gacgctctat tcgacgctca agcagctcgc taccgccgag gtcaacgtct gcacgatcga
    14941 agacccgatc gagatgatcg agccggcctt caaccagatg caggtgcagg gcagtatcga
    15001 catggatttc gccgatggcg tacgggcgct gatgcggcag gacccggaca tcatcatgat
    15061 cggcgagatc cgcgatttgc cgactgccga aatggcgatc caggccgcgc tcaccggcca
    15121 tctcgtgctc tcgacgctgc acaccaacga cgcgccggcg gcaatcaccc gcctgctaga
    15181 cctcggcgtg ccgccctacc tgatcaacgg cacgttgctc ggcgtcatgg cgcagcgact
    15241 ggtgcgcacg ctctgcccac attgccgcaa gcctaacgca ccgcgtaccg aggacgaagg
    15301 catgtggcag gccctggtca gtccgttcaa ggcggcgatg cccaagcagg tgcagcattc
    15361 ggtgggctgc ctcgaatgcc ggcggaccgg ctatctcggc cgcgtcggcc tctacgagat
    15421 catggtcatg tcgcccgagc tgaagaagct cgtcaccgac accaccgaca tcgccaaact
    15481 tcgcgagcag gcctatcgtg aaggcatgaa gccgctgcgc atctcggggg cgatgaaggt
    15541 cgccagtggg cagaccacgc tcgacgaaat cgttcgcgtc gcgccgccgg gcgacggttt
    15601 ttagcggccc gcgtcggcgg cagaccgcca ggtttccctg cgccggcgct agcgggccgg
    15661 gccgtccgcc gagaattcgc cgtcgacatc gccgtagcgg tcgggtgacg gcgatgtcga
    15721 ttcttcggga tgcgtttccg aatccgcccc atcttcggcc gccgcttcgt tttcggcatc
    15781 gacctgtggc gccgccagca ggtgcgctgc cttgtcggct tgatcgatcg cccggcgcag
    15841 cgaactcagg cggcgcagca gcgcgaccat cgcgtcgatc tccaactcgc ctcgcgcgcc
    15901 ggcggcgagc agcgccgcct tgagcggctg gtagtgcgac tcgaaacgtt cgcgctggtg
    15961 cgcgctggcc ggcgcgacgg gcgccgtatc gggcgccagc tggcgcagca gttcgccggc
    16021 ctcgtcggtg agctcggtga cggaatcacg taactctgca tcgagccggc cgtccatcgt
    16081 tgccactgca tcggcttcgg ctgccagctc ggcgcactgg tcgtagtagc gctgcgcacg
    16141 cagcaggcgg gccagtgcgt cggcactcgc tgccgacatt acgccgcggt gcatgcgcgt
    16201 tacgaagccg ctgatcgcgt ggctcagcgc cgctgcggcg gcggcgctgc cgtgtacgcc
    16261 gggcgcttgc gccagccggg tcagcacggc gccaagcgaa aggccgccaa ggcgaacgat
    16321 ctcgcggcgc agcgcgtcaa cggccagcga gggcacgctg gcgacgttgg cgtcgagata
    16381 gcgcggctgc gcgagatcgt cctcgcggcg gcggaagcgg tgctgtagga agatcgccag
    16441 ccgattcgcc agcggccaca tcaggacgac gccgaggaca ttgaaaaccg tgtggaacag
    16501 ggccagcgtc acggccggcg ccgcgcccat ctcgagcagg gcacgcagcc aggcgaccag
    16561 gccgaccaga aagggcagca gcagcagcgc gaccaaaccc gtcaccacat tgaacagcac
    16621 gtgcgctgcc gccgcgcgcc gggcgttggc cgtcgcgccg accgccgcca gcagcgccgt
    16681 caccgttgtc ccgacgttgg caccgatcac cgccgccgcg gcctcgccga tcggcagcac
    16741 gccggtctcg gcgagcgtca gcaccacggc cagcgaggcg ctcgacgact gcatgacgat
    16801 ggtcagcagc atgccggcga aaacgaaggc gatcaccgag cgccagccga ggccgccgaa
    16861 ggccgcgagg tcgaccgccg agccggcgcc cgagaacgcc tgctgcagga agccgatgcc
    16921 gaggaagagc acgccgaagc cggccagcgc gccaccgatc gcaccgcgcc gcgtctccgg
    16981 gcccgacagc cgcagcgcca tgccgatgcc gatcaagggc agcgccgccg cctcgatctt
    17041 gatcttcagc ccgaccagcg ccaccagcca gccggtcatc gtggttccga cgttggcgcc
    17101 gaacagcacc cacagagact gagaaaagct taacagcccg gcattgacga agccgatcgc
    17161 cgccaccgtc accgcgctcg acgactgcac catcgcggta acgaagatcc ccgagaacag
    17221 cccgcgccag cgcgtcgccg tggcgctgcc gagcacgcgc ccgagcgtcg gcccggcggc
    17281 ggccttgagg ccgtcggtca tcagcagcat gccgagcaga aagaggccga cgccgcccag
    17341 cgtcaggccg attgcgccct cgctcatgga gacgctgtaa ggaggaggtt catgcggcga
    17401 ggatggcgcc ggcacagcgc ccggtcaagc accgtcgggc gacatgggtt aatgcgcaaa
    17461 tgcgcgcatc gggcttacgc acgcggcagc cgatgcgtgc agcgagctta ctttcctttg
    17521 acttcgagca gttcgatttc gaagagcagc gtcgcgcccg gcttgatcag cgggctcggg
    17581 ctttggttgc cgtaggcggt gtcggccggg caggtgagct tggccttgcc gccgaccttc
    17641 atcatcgcca cgccttctgt ccagcacttg atcacccgat tgagcgggaa ctcggtcggc
    17701 tcgttgcggc tgtacgagct gtcgaactcg gtgccgtcgg gcagcgtgcc gcggtagtgc
    17761 acgcgcacgg tgtcggtggc ggccggtttg gcgcccttgc cgaccaccag cggtagatag
    17821 atgaagccgc tggcggcctt gacagcaccc ttctcggcgg cggcggtggc gagcagcttc
    17881 ttgcccttct cgctctcttc gcgggcggtc tgctcggcac gggattgcgc gagcgcattg
    17941 accttgggcg cgtactgggc gacgtccact gcactcttgg tcttgagcgt tgcgtcgcgc
    18001 cagcccttct ggacatgggt gatctcgtcg gcgctgaggc ggaagacggt ggtctggctg
    18061 gcgagcatct gcccgagcgc gtagagcgtc ttctgctcgt cggtcatgtc ggcggcgagg
    18121 gcgccgtggg tggcgatggc caggctcatc gcgaggggaa tcagtcgttt catggttggc
    18181 ttccgtccgg tttcgggcgt gcccggcggg cacggcgagg ggcaaggata gtgcattgcg
    18241 cagtaggaaa ggccggctgg caacagcgca tcgaatcgcg cgcggctcag gcgaatatcc
    18301 cgtgcagatg ccagccttcg gccgtgcggt agatccaaag gtattcatct tgccggttgc
    18361 gggcgatgaa gtagtcgcgg cggatgtcgc cggtggcgcc gggttcgccg ccgtcccacc
    18421 agcctgattc gatgcgctcg gggccagcga gcagttcgag cgggtcgcgc tgctggtgcg
    18481 ggcggccgtc gatctcgcgc agcgtctccg gctgcggcag cagcagcagc ggtcgcggtg
    18541 gtgccgcggg catggccgtg gtggttgatg cgccgaccgc ggtgtgccgc gtggcgtttt
    18601 ccgggcggtg gccggcagcc ggggagatgc cgtggactgc cgactcgccg aggcgggcgc
    18661 gcaactgctc gacgagcagt tcgatgcctt cgggctcgct gccggccgtg tcgaacagtg
    18721 cgcgcgattt tccggcgagg tcttcgcagt gctcggcgct gagcgtcagg gtttcgaccg
    18781 gcgccggtaa ttcggtgcgt tcgagcagtt cgcgcagcac gcgggcgatg cgttgcggcg
    18841 tgcgcgtcgg cgtggtcagc gtgagcgtga tcatcgattc gcgcggcttg ccgggcagcg
    18901 cctcgtggcc gagcgtcagc gtgcagcgca agacgccctg ctggcgtgcg acgagccagc
    18961 cgcacagcgc gccgattagc cggtcgccgg cgaagcacag cgcgccggct tcgtgggcga
    19021 tgccgggcag cgtcagccgc tggcggaagc gctcgggaaa gacgaagcgc ggccgcaggt
    19081 cggggcgctc gccgagtgcc tgctcgatgc ggaccgcgca gtcgccgccg agacggcggg
    19141 cgagtccggc cagcggcagg cgcagggcct cgcccagggt tttgagcccg aaggaggcca
    19201 gttgctgcgc ctgttgttcg ctggctgcga gcaactgcgc gggcaagggc gcgagcatgt
    19261 caggtagcgc ttcgcgggtg ggaatcacag gtggccggac gccggccgag ggtggcgact
    19321 cgctgccctg cgccagccag agcgcggcct gcggcgttgg ggcggccgcc atgcgcgggg
    19381 cgaggtcgcg ttcgcggcag ccagcgccga cggcggcgag tacggcgtcg atgccgccga
    19441 agaggcgcag gcagccgccg atttcgagca gcagcgcggc gggcggcgcg agcacgatgt
    19501 cgggcgtgta ttgcccggcc cagcaggcga gttcgtcgag cacggtggct tcggcgacgg
    19561 gattgcgcgg cagtagcgcg agttcgggca gcatgccgcg cgcggtggcg gcgcgcatgc
    19621 cgggcatgag gccggcggcc gtggcggtct cgtcgcaggc gagcagccgg ccgcgatcga
    19681 cgacggcgct aggcagcggc cggccggggt gcgcgatcag cggcaggtcg ggtagccaga
    19741 gggcgagcca gagcatgatc ggattccgtg gcgggggtgt tgcgtttgcg cgccggcagg
    19801 gggcgcgcca gcggcagcac gatggggcga tcgcagcgcg ggccgcgacg cttgagtatg
    19861 tgcagcgcgg cgccgctggc ctgcgcggtg atcgtcagcc gcaacggtgc cggcgaggac
    19921 tggtcggcag cggctgccgg cagcatcagg aagccgctac cgccgccggc ctcggcagcg
    19981 agttgcaggc gcttgcgcgc ggtgaaggag agcattcctg gccagacgat cgtcgcggcg
    20041 accgcgccgc tgcgcaggca gcgttcgctg gcccagttgc gttcttgggc gtcggcggtg
    20101 gcgacgtgca gcaggtgcgc gagatcgatc ccgtgctgca gcagtgccgg cgcatgcagc
    20161 ggcagcggcg gggcgatcag ggccacccag cggccggcac gggtgagtcg ggcgatcgcc
    20221 ggcaacagca gcgacagggc gccgttgccg cgcatcgccc cagccctcgc cgggtcgcgt
    20281 tgcgaggcgt aggtggcagc gctgccgggc atcagttcga cgagcgcacc ggcgggccag
    20341 ccgccgccgg gcagttcggc atcaagtacg gcgaagccgc tggcgatcgt ggccgcggtg
    20401 ctgccggcga gttgcgcgcc acgccagaga tcggcgcggt ggagcagcgc gtcgagcgtg
    20461 atcggcggga tgccgggccc tgccgatggg cggcttgccg attctgccgt gggcgctggc
    20521 gtgacgccgg ccggggtttc agccgatgcc ggtgacgggg gtggcccgag gggccggaga
    20581 gcgcggacag acatggcgtc ggctcatcgc gtccgggcgt tttcgacgcc ctgcttgcgc
    20641 agcgttcgca gcgcgtgatg gacgatccat cgcgtttcgc tggtcggcga ttcggcgagc
    20701 cagcgcgcgg tggtctgccg cacccagtcg ggctggtgct tcgagacgtc gttgagccag
    20761 ttggcgaccg agagctgcac gtagcggctg gtgtcgctgc gcacggcttc gagcagcggc
    20821 agcgcgagtt gcggggtttg cttcagggca tcgatatgga cgcaccagac gccgcgtggg
    20881 cgcgtcgctt cgatcgcgca gcggcggatg ttggggtcgg catcgcgcgt ccacggcgcg
    20941 agcagcgcta cagcgttgtc gagatcctcg gcgagccagg ggcggaagga cgaccaggcg
    21001 atctcgcgca cgttcatcga cggatcggcg gcgaagcggc gggcgccggt gagccgcgtg
    21061 gcgaggtcgt agccggggtc ggcagtccag catagcgccg cccattcgcg caccacgccc
    21121 gaagcgtggg cggcgacgca ttcgtagcag gactgcggct cggccgcggc gcggatcagc
    21181 ggcaggagcg cggcgccgat tgcccgctgg cgggccatga cgccgtcggc tgaatcggcg
    21241 acggcgatga gttgatcgac ggcctgaccg agtccggcgc cgggcagggc cgccgcgagc
    21301 agcttgcggt aatcgacggc cagccactcg gccagcgtca gcggttcctc gcggccgctg
    21361 tcgagcgccg cctgcagatc ggcagggatc gcggcgcggg cattgtaggt tttccgtttc
    21421 actggacgac catcccacag gcactcttcg accggaagag ggcggcggcg caggctacag
    21481 cgcttgcccg ccgcggatca ggccgacggc gatgccttcg atcgtcagcg cttcgcgtcg
    21541 cgtgtcgacg atgatcggct cgaagtcggg gttctcggcg atcagctcga ccgtatggcc
    21601 cttgcgccgg aggcgcttga cggtcacgtc gtcggagatg cgggcgacga ctacctggcc
    21661 gttgcgcgcc tcgctggtct tgtgcacggc gagcaggtcg ccgtcgagca tgccgatgcc
    21721 gatcatcgac tggccgcgca cacgcagcag gaagtcggcc ttgggggtga acagccgcgg
    21781 gtcgacctgc aggtgtccct gcacatgttc ggtggcgagc agcggcgagc cggcggcgac
    21841 gtggccgatc agcggcaggc cgagctgctc gaccagacgg atgccgcgcg ccgagccggg
    21901 ctcgaggtgg atgacgcctt ttcgggcgag cgccttgagg tgatcctcgg cggcgttggc
    21961 cgaagcgaag ccgaaggcgc tggcgatctc ctgccgcgtc ggcggcgcac cgagcatttc
    22021 gacggtgttc ttgatgaaat cgaggatttc ctgctgccgg ggagtgagct tgaccatgac
    22081 gggcctctgt actgtacgca aacacagtat agccaaacgg cagtggtgcg caagggccgg
    22141 gcgaagctgg tggtcgcggt gagggcagag atcgaggcgt tctgtctcgg ccgcgcagcg
    22201 gaaccgggta accgtggcgc gttctatccg gtatcggctg tgacttggtc cgcgtcagcg
    22261 tttcggccgc tccttccgca gaaatcggct atgcaagcaa aacgactgat tgcactggcg
    22321 gcgctggcct tggcaggttc tgcccaggcc gcgatcggag attgcgctgc tgcgcgcgcg
    22381 aatgccggcg catcagactg cgccgcgccg aattccgcca gcgccggcta tcgcccctgg
    22441 aaagaaaccg gcgggcccgt gtgtcgtgcc ttcgccagct accttgaccg cctgccgcgg
    22501 atcgaccccg cgctcacctg cgacctcgat ggtcggctgg ccggagtcgg catcacgcgg
    22561 ccacagtggg tcgaactcga tcccagcgaa taccgcgatg tcgtttacgc catcgaaatg
    22621 tcgctggcgc ccagaagccg tggcggccag gaacggctcg ccgggccgat cgccgagcac
    22681 ccgccacggg ccgacggacc ctacacgatt cctttcgacc gctggcgccg caactacgcg
    22741 agcaacgtgc aggcgggcca cctcaggccg cggctgcgca tggccacgct gcagctctcg
    22801 ccgccggacc cgccggtgac ggtactcgcc tacacccccc agcgtgacga cgagctggcc
    22861 tgccaagatc ccggtgccgt caacaacatg ctgtcgcgaa accgactcgg cgatcacttg
    22921 ttcccctatg acccggcgac gcgaacgctg cggcagatcg tatcgagcga agtcgtcgtc
    22981 agcagccgcc tgcgctaccg ggccgtggtc ttcgacggcc gtgcgtatct catcggcgtc
    23041 ggcgccgccg cgccagctgg cgcctttacc gtcgagttct cgcgggtcga acgctacggc
    23101 agcgatggcc cgcaggaccc gctgtacgcg ggctaccacc tctgcggctt ctccctggcg
    23161 ggtgaaaacc ggcaatgacc acccgccgaa tcatgtcttt tcgttgatcg gcatcaatta
    23221 cggcggcgaa gcaagcccaa acggcaacgg tctcaggacg gcgcgacgtt ccgcacctat
    23281 agtgaaagcg agggggaggt ggtttggact ttgcgcctcc gaagggagcc gatcatgtcg
    23341 atcttggatg taccgatttc ccgctgcgag gcaatgcgta ccatggtggt gaccgatcag
    23401 acccaggcgc agtgcgcgcg cgagcatcag tgtccgccgg gcatggcctg tccgctctgt
    23461 ggctgctttt ccggcgacga ctggcgcgag gtggtggtgg cgaaggagtc gagcgcgcag
    23521 tcctagtttg gttgccgcga tgccgccaac acgccatcag gagtaggccc gccgacaaca
    23581 ccgcatgcgc gcctgccgct acaatcggcg ctttccctac ggattgaagc gccgcgatgc
    23641 gaatcctcat cagcaacgac gacggctatt tttcgccggg catcgaggcc ttggccgcgg
    23701 cgctgcgcga ggtggcgcag gtgaccgtgg tcgcccccga gcgtgaccgc agcggcgcca
    23761 gcaattcgct gacgctcgat cgtccgctga tgctgcgcaa ggcagccaac ggcttctact
    23821 ttgttaacgg cacgccgacc gactgcgtcc atctcgcggt gaccggcatg ctctccgaac
    23881 tgcccgacat ggtggtctcg ggcgttaatc tcggcgccaa catgggcgac gacacgatct
    23941 attcgggtac agtcgccgcc gccaccgaag gcttcctgct tggcgtgccc tcgctggcgg
    24001 tttcgctcaa cagccgcaac gaatcgaatc tgggcgctgc cgcagcggtg gcgcgcgagc
    24061 taatcgagcg catcagccag cagcgtctct cggggccgat gctgctcaac gtcaacgtgc
    24121 ctgacctgcc gcgcgagcag atcaagggca tcgtgacgac gcgcctcggt cgccggcaca
    24181 aggccgaacc cgtggtgaag gcgaccaatc cgcgcggcga aacctgctac tgggtcggtg
    24241 ccgccggacc ggcgcaggat gctggcgacg gcaccgattt ccatgcggtg ttgtccggtt
    24301 ttgtttcggt cacgccgctg cagatcgatc tcactcacaa cggccagatg ccgatcgtca
    24361 agcagtggct gcagtgacat ggtggcggta agactgcgtg gcgaccgcgg cgccgggatg
    24421 acctcggagc gcacgcgggc gcgcatgatc gatcgcctgc gcgagcgagg gatccgcgac
    24481 gaggccgtgc tcaaggcgct cgccgcggtg ccgcggcacg tgttcatcga cgaggcgctg
    24541 gcgcatcgtg cctacgagga cacggccctg ccgctcgggt gcgggcagac gatttcgcag
    24601 ccctacgtag tcgcgcgggc gatttcgctg ctgcgtgccg ggcgcgagtc gcggctcaag
    24661 ggcggtctgg gcaagacgct ggagatcggc gccggctgtg gttaccaggc ggccgtcctt
    24721 gggtgtctga cgcccgatgt ctatgccgtc gagcgcatcc gcgagctgat cgagcgggcc
    24781 aaggtgaacc tgcggacgat ccagcagttc aacgttcgcc tgaagtttgc cgacggcaat
    24841 ctcggcgttc ccgaagccgc gcccttcgac acgatcattt cggcggcagc aagtgcaata
    24901 gtaccgccgg tgcttttgca gcagttgaac gttggtggca gaatccttct gcctctcggt
    24961 ggcgccgacc agcagctcgt gatgttcgag cagacagaaa aaggcctcgc cgaatcgcgt
    25021 ttcgatgcgg tgcgcttcgt gccgctccta caggggactc aatgaccgat cgccgcgcac
    25081 cgcgccttgc cctgccgttc gtgctcggac tgtgtttggt gttgggggcc tgcgcatcga
    25141 agtcgccggc acccatcgtt gatcgttcgc caaccggctg gatgagctgg cccgcggcat
    25201 cggcggccag cgctgcgccg ccgccggtat cgactaatgc gatcgtcccc gtcgaggcgc
    25261 cggccggtgt cggttatcac cgcgtggcca agggcgacac gctctacagc atcgcgctcg
    25321 acaacggtca ggattacaag gaagtcgccg agtggaacaa gctcgccgac ccgtcgcaga
    25381 tcaacattgg gcaggttctg cgggttattc cgccgggcgc agtggtcgcc ggcaacggcg
    25441 tcgccgtatc gcagccgatc ggtagcggat cggcggtcga gaccgctccg ctcgattcag
    25501 gccgggcgcc ggcggtcgtt gcgaagtcag cgccggcagc cgtgtcggcg ggcgggcgct
    25561 gggcatggcc ttcgtcgggc aacctgatcg ccgggttttc cggtgacaag aacaagggca
    25621 tcgacctcgc cggcaaaccc ggcgatccgg tgagcgccgc cggcgacggc aaggtcgtct
    25681 acagcggcag tggcctgcgc ggctacggca atctggtgat catcaagcac gacgccgaat
    25741 acctgaccgc ctacgcccac aaccgcaaga ttctcgtgcg cgaaggccag caagtgaagc
    25801 gtggcgagaa gatcgccgag atgggcgact ccgatgccga ccgcgtcaag ctgcatttcg
    25861 aattgcggcg gcagggcaag ccggtcgatc cgcttaagca tttgccggca cggtagcaat
    25921 ggtcagcggc caggattccg aagaagaagg cgtcgtcgaa atcgatgccg cagtcgacac
    25981 ggaaggcggc gccgatgaat tgaccatcga gccgcgggcg gcggcagccg gcgagagcga
    26041 tctgatcaac gatgtcaccc agctctacct caacgagatc ggctcacggc cgctgctgac
    26101 gccggccgag gagttcgact tcgcgacaaa ggcgcgcgcc ggtgatttcg aggcgcgtca
    26161 gaagatgatc gagcacaacc tgcggctcgt cgtgaacatc gccaagcact acacgaaccg
    26221 gggaattccg ctgctcgacc tgatcgagga gggcaacctc ggcctgatcc acgcgatcga
    26281 gaagttcgag cccgagcggg gcttccgctt ttcgacctat gcgacctggt ggatccgcca
    26341 gagcgtcgag cgcgcgatca tgaatcagtc gcggaccatt cggttgccgg tgcacgtggt
    26401 caaggaaatc aacgtggtgc tccgcgccat ccgccacctt gaggcggcga actcgcgcga
    26461 gaccggcgtc gaggagatcg cgcacctgat cgacaagtcg gtcgatgagg tacgccacat
    26521 cctgcaactc aacgagcaca tcgcctcact cgatgcgcca ctcgacatcg atcccagcca
    26581 caacatcggt gaactgctcg ccgacgagca gagccgcgac cccgaagccg aactggccgg
    26641 gcatgaactc ggtgcgctgg tgcatcaatg ggtcgaccaa ctcgaaccca agcatcggct
    26701 tgtggtcgag cgtcgctacg gcctcaacgg ccatgagatt gccacgctcg aatcaatcgc
    26761 cagcgaactc gggctgaccc gcgagcgggt gcggcagatt caactcgaag cgctgcagaa
    26821 gctcaggcgg atcatccgcc gcggcggggt gtcgcgggag agtctgctct agcggtttgc
    26881 tccgaacgcg cgtcggcgac agcctgaccg agcaaattga cagccattgc gtacgacgag
    26941 ctgcggttgt agcggctaat cacgtacagg ttgtagaacc cccaccagta ctcaacggcg
    27001 tcgtccgagc gcagctcgat cagtgacacc tcctcgggcg cacggtcgga cagtgtggtc
    27061 cacagcgaca tcggcccgga cagcgagggc aggatgcccg ccaccaacca gtgcgagggg
    27121 ggcggcgagt cgaacttcgc cggatgggcg atccgcccgc ctggcttcca gccatggatc
    27181 tgtaggaagg ccgcgacgct accgatggcg tcgcgcgggc tgttggcgag atcgatccgc
    27241 cggtcgccgt cgaagtcgat cgcgtagctg cgaatgctcg tcggcatgaa ctgcggaatg
    27301 ccggtggcgc cggcatagga actcttgaac gactcgggtg gcagctgctg ctcgcgggcg
    27361 tggagcagga aggccgccaa ctcgcgcttg aaataggt
//
LOCUS       DATDAA010000140         2803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_290179,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000140 DATDAA010000000
VERSION     DATDAA010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290179"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..896
                     /gene="lgt"
                     /locus_tag="VIS73_04580"
     CDS             48..896
                     /gene="lgt"
                     /locus_tag="VIS73_04580"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY5762465.1"
                     /translation="MLVHPQFDPVAIALGPLAVRWYGLMYLAAFLQFLWLGRRRLASG
                     LFPAWTKEDIDDLLFAGVIGVVIGGRLGQVLFYDPGYYAANPLEIFAVWKGGMSFHGG
                     LLGVVVAEVWFARRKGRSLLEVADFVAPLVPLGLAFGRIGNFINGELWGRVADASLPW
                     AMVFPHVDPLPRHPSPLYQAGLEGLTLFALLWWYAAKPRRPGQVAGFFLAGYGVARFI
                     AEYFREPDAGIFGRSELISMGQWLSLPMVALGLWLVLRTGPADRIPASRPEATDNQRR
                     RKGKRK"
     gene            893..1369
                     /locus_tag="VIS73_04585"
     CDS             893..1369
                     /locus_tag="VIS73_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5762466.1"
                     /translation="MSQRPFRVLGIQQIAIGGPSKSRLSTLWVEKLGLNVTGTFVSER
                     ENVDEDICAIGSGPFKVEVDLMQPLDPEKKPAVHTTPLNHVGLWIDKLPEAVEWLAAE
                     GVRFAPGGIRKGAAGFDICFLHPKANDEFPVAGEGVLIELVQAPPEVVEAFDRLGC"
     gene            1386..2558
                     /locus_tag="VIS73_04590"
     CDS             1386..2558
                     /locus_tag="VIS73_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762467.1"
                     /translation="MGQDSGTRRAQARFQSGLLAWLRSSGQPEGLRDMVAVVRELSAD
                     DGAPGDKMLWRSADSFLAALLDGTLSPDDEAKGLARRIERRLASGSAETRSADDASLR
                     EALFAWISRRTLPPGNERASIAVADLPAAGLSSTLAATAELLPLLGGRLLPARCSDEQ
                     LKQWTAGAEALDRAWSALLRDGLDPCRKAATTFVSTALEIGDPACLKLAEALATAVGC
                     AEDPAWRDAAALRAAIAGALDLARDRQGPNHPSFGVRTDELVRRIETAEEGLRSARRD
                     SGATGKHEAFIATARERLKAVATLLEATPSASENLREALGWFETQRFGNSMAIRGLVV
                     LWREALAKGRPEALAAPGRQALAALGEALDGLALGRAPHPDIAAFAALRAVHRAVS"
     gene            2607..>2803
                     /locus_tag="VIS73_04595"
     CDS             2607..>2803
                     /locus_tag="VIS73_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762468.1"
                     /translation="MAWFDLQSQASQHAPAFDSAESAKKWLGSQSQTVPARMQIALAE
                     QFEALHGAGLPPLELARILSEL"
BASE COUNT          434 a         1016 c          927 g          426 t
ORIGIN      
        1 ggataatcct cagctagctg agactccgct aggcagaaaa gaatcccatg ctcgttcatc
       61 cgcaattcga ccccgttgcc atcgcgctcg gtccgcttgc cgttcgctgg tacggcctga
      121 tgtaccttgc tgccttcctg cagttcctct ggctcggccg gcggcggctg gccagcggcc
      181 tcttcccggc ctggacgaaa gaggacatcg acgacctgct gttcgccggc gtgatcggcg
      241 tggtcatcgg cggccggctc gggcaggtgt tgttctacga ccccggctac tacgcagcga
      301 acccgctgga gatcttcgcc gtgtggaagg gcggcatgag cttccacggc gggctgctcg
      361 gcgtggtcgt cgccgaagtc tggttcgccc ggcgcaaggg ccgctcacta ctcgaggtgg
      421 ccgacttcgt cgcgccgctg gtgccgctcg ggctggcctt tggccgcatc ggcaacttca
      481 tcaacggcga actgtggggt cgcgtcgccg acgcctcgct gccctgggcg atggtctttc
      541 cgcatgtcga cccgctgccg cgccacccca gcccgctgta tcaggcgggg ctcgaaggcc
      601 tgacgctgtt cgcgctgctc tggtggtacg cggccaagcc gcgccggccc gggcaggtcg
      661 ccggcttttt cctcgcaggc tacggtgtcg cccgcttcat cgccgagtac ttccgagagc
      721 ccgacgccgg catcttcggc cgcagtgagc tgatcagcat gggccagtgg ctgagcctgc
      781 cgatggtggc gctcggcctc tggctagtcc tgcgcaccgg gcccgctgat agaatcccgg
      841 cgagccggcc ggaagccacc gacaatcaac gacgacgcaa gggaaagaga aaatgagcca
      901 acgtccattc cgcgtgctgg gcatccagca gatcgccatc ggcggcccct ccaaatcacg
      961 tctgtcgacg ctctgggtcg aaaagctcgg actcaacgtc accggcacct tcgtcagcga
     1021 gcgcgaaaac gtcgacgagg acatctgcgc gatcggcagc ggcccgttca aggtcgaggt
     1081 cgacctgatg cagccgctcg atcccgagaa gaaacccgcc gtgcacacga caccacttaa
     1141 ccacgtcggc ctgtggatcg acaagctgcc cgaggccgtc gaatggctcg ccgccgaggg
     1201 cgttcgtttc gcgccgggcg gcatccgcaa gggcgccgcc ggtttcgaca tctgcttcct
     1261 gcaccctaag gccaacgacg aattcccggt cgccggcgaa ggcgtgctga tcgaactggt
     1321 gcaggcgcca cccgaagtcg tcgaggcctt cgaccgcctc ggatgctaag cgcccgggaa
     1381 cccccgtggg ccaggacagc ggcacccgac gcgcccaagc gcgcttccag agcgggctgc
     1441 tcgcttggct gcgctcgtct gggcaacccg aggggctgcg cgacatggtc gccgtggtcc
     1501 gcgagttgtc ggccgacgac ggcgctcccg gcgacaagat gctctggcgc agcgccgaca
     1561 gttttctcgc ggcgctgctc gacggcacgc tgagcccgga cgacgaagcg aaaggcctgg
     1621 cgcggcgcat cgaacgccgg ctcgcgtccg gcagcgccga gacgcgctct gccgacgacg
     1681 ctagcctgcg cgaggcgctg ttcgcctgga tttcccgccg cacgctgccg ccgggcaacg
     1741 aacgcgcctc catcgccgtt gccgacctgc cagcggccgg gctgtcgagc acgctcgcgg
     1801 ccaccgccga actgctgccg ctgctcggcg gccggctgct gccggcacgc tgcagcgacg
     1861 agcaactgaa acaatggaca gccggcgccg aagcactcga tcgcgcatgg tccgcgctgc
     1921 tgcgcgacgg cctcgacccc tgccgcaagg cggctaccac cttcgtctcc actgccctcg
     1981 aaatcggcga cccggcctgc ctcaagctcg ccgaggcact ggccaccgcc gtcggctgcg
     2041 ccgaggaccc ggcctggcgc gacgccgcgg cactgcgcgc agcgatcgcc ggcgccctcg
     2101 atctggcgcg cgaccgccag ggaccgaacc acccgagctt cggcgtgcgc actgacgaac
     2161 tggtccggcg cattgagact gccgaagaag ggttgcgcag cgcccgccgc gacagcggcg
     2221 caaccgggaa gcacgaggcc ttcatcgcca ctgcccgcga acgcctgaag gcagtcgcca
     2281 cgctgctcga ggcgacgccg tcggccagcg aaaacctgcg tgaggcgctc ggctggttcg
     2341 agacccagcg cttcggcaac agcatggcga tccgcgggct cgtagtgctc tggcgggaag
     2401 cgctggccaa gggccgcccc gaagcactgg ccgcccccgg ccgccaggcg ctggcggcgc
     2461 tcggcgaggc gctcgacggg cttgcgctcg gccgcgcgcc ccatcccgac atcgcggcct
     2521 tcgccgcgct gcgggcggtg caccgcgccg tgtcttgagt atgtagtcgc gctaaactgc
     2581 tggctccgca ctcaacggaa tcgctcatgg cctggtttga cctgcagagt caagcatcgc
     2641 aacacgcccc ggcctttgac agcgccgaaa gcgccaagaa gtggctgggc agccagtcgc
     2701 agacggtccc ggcgcgaatg caaatcgccc tcgccgagca gttcgaggcg ctgcatggcg
     2761 ccggactgcc gccgctcgag ctggcacgca tcctcagcga gct
//
LOCUS       DATDAA010000141         3778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_149811,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000141 DATDAA010000000
VERSION     DATDAA010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149811"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VIS73_04600"
     CDS             <1..905
                     /locus_tag="VIS73_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEY5762469.1"
                     /translation="RSGVDRRRFLAVMAAAAANSPVRAVAAAGAYETGLLWRIDKPGV
                     RPSYVFGTMHLPDARLLPLPEPVADAFDACRRLIVEMLPNPAVARRFAEAMQLESGAT
                     LDALLSSAHFGRLVERLAVRGYSRESVRQLKPWAALLQVSARPADAAGEKSIDTELFL
                     RARFANKSVEELDTVEEQIAVFDDIPLTSQLSLLAAAIDFHPRLAESAEQVMQAYLAR
                     DLAEITRAGRLLERERPELAAHQRMLEKKVIADRSVVMAYRLQYYLRRGESFAAIGAS
                     HLHGPAGVPALLADEYEWRVRRLW"
     gene            915..1742
                     /locus_tag="VIS73_04605"
     CDS             915..1742
                     /locus_tag="VIS73_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY5762470.1"
                     /translation="MTAPETSIQVAPLDLAPIIDIPPLEILYTDESLLAVHKPSGLFV
                     HRTTLDARVEDCLLRRVRDFVGAQVHAVHRLDRGTSGIVLFARSSAAAAALSAQFEAG
                     RVEKRYLAIVRGHPPHEGRIDHALVPLDPDGNRLRHLPAQAAVSDYRVLATVELPIEV
                     DRYPRTRYAFVELRPLTGRRHQLRRHLKHIAHPVIGDATYGKGVHNRFIAGYSGVSRL
                     LLACTAVGFTLPESGEHVLLEAAPAADFRRVADALGWQAVIESAGELERADADAPAG"
     gene            complement(1817..1972)
                     /gene="rpmG"
                     /locus_tag="VIS73_04610"
     CDS             complement(1817..1972)
                     /gene="rpmG"
                     /locus_tag="VIS73_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY5762471.1"
                     /translation="MRDKIKLESTAGTGHFYTTTKNKKTMPDKMEIKKYDPKARKHVA
                     YKETKLK"
     gene            complement(1983..2216)
                     /gene="rpmB"
                     /locus_tag="VIS73_04615"
     CDS             complement(1983..2216)
                     /gene="rpmB"
                     /locus_tag="VIS73_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY5762472.1"
                     /translation="MARVCQVTGKGPMVGNNVSHANNKTKRRFLPNLQYRRFWVESEN
                     RFVRLRVSNAGLRTIDKKGIEVVLADLRARGEI"
     gene            complement(2297..2792)
                     /locus_tag="VIS73_04620"
                     /pseudo
     CDS             complement(2297..2792)
                     /locus_tag="VIS73_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236297.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
     gene            2895..>3778
                     /gene="coaBC"
                     /locus_tag="VIS73_04625"
     CDS             2895..>3778
                     /gene="coaBC"
                     /locus_tag="VIS73_04625"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY5762473.1"
                     /translation="MELQGRRIVLGITGGIAAYKAAELTRLLVKAGATVDVVMTEAAT
                     HFVTPVTFQALSGRPVVTDQWDARFAANMAHIDLTRGADLVLVAPASADFMARTANGL
                     AGDLLGTLVLARPAHCPLLVAPAMNREMWDKPATQRNVTTLHADGVTLLGPAAGEQAC
                     GETGDGRMLEAVELYEEVVAHLQAKLLAGRRVLITAGPTFEAIDPVRGLTNLSSGKMG
                     FAVARAAREAGAEVVLVAGPVSRTTPRGVRRIDVTSAAEMHAAVIAEVGAADIFIGIA
                     AVADYRPAVVAHDKIKKGG"
BASE COUNT          587 a         1248 c         1284 g          659 t
ORIGIN      
        1 ggaggtctgg ggtggaccgt cggcgctttc tcgcggtgat ggcggcagct gcggcaaaca
       61 gtccggtgcg tgccgttgcc gcagccggtg cctacgagac cggactgctt tggcggatcg
      121 ataagccggg cgtacggccg agctacgtct tcgggaccat gcatctgccc gacgcgcgcc
      181 tgctgccctt gcccgagccc gttgccgatg cctttgacgc ctgccgccgg ctaatcgtcg
      241 agatgctgcc caatccggcc gtcgcccggc gtttcgccga agcgatgcag ctcgaaagcg
      301 gcgcgacgct cgatgcgctt ctgtcgagcg cgcatttcgg gcgactggtc gaacgactcg
      361 ccgtgcgcgg ttattcgcgc gaatcggtgc ggcagctcaa gccctgggcg gcgctgctgc
      421 aggtcagtgc gcggcccgcc gacgcggcag gcgagaaatc gattgatacc gagctgttcc
      481 tgcgcgctcg cttcgccaac aaatcggtcg aggaactcga cacggtcgaa gagcagatcg
      541 ccgtcttcga cgacatcccg ttgacctcgc aactgtcgtt gctcgcggcg gcaatcgatt
      601 tccatccgcg gcttgccgaa tcggccgagc aggtgatgca ggcctatctg gcgcgcgacc
      661 tcgcggagat cacccgcgcc ggccgcctgc tcgaacgaga gcggcccgaa ctcgcggcgc
      721 accagcgcat gctggagaag aaggtgatcg ctgaccgcag cgtggtcatg gcctaccggc
      781 tgcagtacta cctgcgccgc ggcgagagct tcgctgcgat cggtgcctcg catctgcacg
      841 ggccggccgg tgttccggcg cttctggccg acgaatacga atggcgtgtg cggcggctct
      901 ggtagcctgc gccgatgact gcgcccgaga catcgattca ggtagcgccg ctcgacctcg
      961 cgccgatcat cgatattccg cccctcgaaa tcctctacac cgacgagtcg ctgctcgccg
     1021 tgcacaagcc ctcgggcctt ttcgtccatc gcaccacact cgacgcgcgg gtcgaagact
     1081 gcctgctgcg gcgcgttcgc gatttcgtcg gcgcccaggt ccatgccgtg caccggctcg
     1141 accgcggcac ctcgggaatc gtcctcttcg cccgttcgtc ggccgcagcc gcggcgctgt
     1201 cggcgcagtt cgaggccggc cgcgtcgaaa agcgctatct ggcgatcgtt cgcggtcatc
     1261 cgccgcatga gggccgaatc gatcacgcgc tggtgccact cgatcccgac ggcaatcgct
     1321 tgcgccacct gccggctcag gccgctgtca gcgactaccg ggtgctggcg accgtcgaac
     1381 taccgatcga agtcgaccgt tatccgcgca cacgctacgc gttcgtcgaa ctccgtccgc
     1441 tgaccggccg gcgccaccag ttgcggcgcc acctcaagca catcgcacat ccggtcatcg
     1501 gcgacgcgac ctacggcaag ggtgtgcaca accggttcat cgccggctac agcggcgtgt
     1561 cgcgcctgct gctcgcctgt acggcggtgg ggttcacgct tccggaaagc ggcgaacacg
     1621 tgctgctgga agcagcgccg gcggcggatt tccggcgcgt cgccgacgca ctcggctggc
     1681 aggcggtgat tgagtcggca ggtgaactcg agcgcgccga tgccgacgct ccggcagggt
     1741 agcggtcagc cggcgggatt cgccaaaagc aaaggcccgc ttgcggcggg cctttcaagg
     1801 atgctcagga aaccgcttac ttgagcttgg tttccttgta ggcgacgtgc ttgcgtgcct
     1861 tgggatcgta cttcttgatc tccatcttgt cgggcatcgt cttcttgttc ttggtcgtcg
     1921 tgtagaaatg gccggtaccg gccgtcgact cgagcttgat cttgtcgcgc atgatgggtt
     1981 cctcagattt cgccgcgggc gcgaaggtcg gccagcacga cctctatgcc cttcttgtcg
     2041 atggtgcgca ggccggcgtt ggaaacgcgc aggcgcacga agcggttttc gctctcgacc
     2101 cagaagcgac ggtactgcag gttgggcagg aagcggcgct tggttttgtt gttggcgtgg
     2161 gaaacgttgt tcccaaccat cgggcctttg cccgtcactt ggcagactcg cgccatggct
     2221 gttgctccgg aattcttgga aagaaagccg ggcattctat gtgaaaacgt ctccgctcgg
     2281 caaggcctat cttcgactag acaaggccgc gctcggtcag cgataacggc tcgcagccgg
     2341 cagcgaccac gaaatggtcg agcacgcgaa catcgacgag cgccagtgct gccctcaggg
     2401 cctgggtgat cgcctagtcg gctgccgagc aggcggtttt gcgcgtcgag caggagcagc
     2461 atgaagactt cgtggggcag gtgaccgatt gccagccgca ggtaatcgcg gaccgacgcc
     2521 agcgaggcga acgagtcggg gtcgcgcagg cgcttgccgg gcgcgcgctg ggcgatctcg
     2581 agactggcgg ccagttgcgc cgccttggcc gggccggttc cctttgtctg cggcaactcg
     2641 ccgacgctgg cgcgacagag ccgttcgagg cgtccatcgt aggcggccag taattcgcgg
     2701 gcgaggtcga cggcgccctt gccgggaatg ccggtacgca gaaatacctc cagcagttca
     2761 gcgtcgctga gtgccgcggc gccgtcgcgc agcggccact agcgcgggcg gtcggcctcg
     2821 ggccagtcgg tgatcggcat tgccgcggcc atccagtaga atcgacgact cgaagtgtag
     2881 ccggattcaa gacaatggaa ttgcagggca gacgcatcgt gctgggcatc accggcggca
     2941 tcgccgctta caaggcggcc gagctgacgc gcctcctggt caaggccggt gccaccgtcg
     3001 acgtggtgat gaccgaggcg gcgacgcact tcgtgacccc ggtgaccttt caggcgctgt
     3061 cgggccggcc ggtcgtgacc gaccagtggg acgcgcgctt tgccgccaac atggcccaca
     3121 tcgacctgac acgcggtgct gacctagtgc tggtggcgcc ggcctcggcc gacttcatgg
     3181 ctaggaccgc caacggcctc gccggtgacc tgcttggcac cctggtgctg gcgcggccgg
     3241 cgcactgccc gctgctcgtg gcgccggcga tgaaccgcga gatgtgggac aagccggcga
     3301 ctcagcgcaa cgtcacgacg ctgcacgccg acggcgtgac gctgctcggc cccgccgccg
     3361 gtgagcaggc ctgcggcgaa accggcgacg ggcgcatgct tgaagccgtg gagctctacg
     3421 aggaagtggt cgcccacctc caggccaagc tcctggccgg tcgccgggtc ctgattaccg
     3481 ccggaccgac cttcgaggcg atcgacccgg tacgcggatt gaccaatctt tcctcgggca
     3541 agatgggctt cgccgttgcc cgcgccgccc gcgaagccgg ggccgaggtc gtactggtcg
     3601 ccgggccggt gagccggacg acgccgcgcg gggtgcggcg tattgacgtg accagtgccg
     3661 ccgagatgca cgcggcggtg atagccgagg tcggtgcggc cgacatcttc atcggcatcg
     3721 ccgccgtcgc cgactaccgg ccggcggtag ttgcgcacga caagatcaag aagggcgg
//
LOCUS       DATDAA010000142         3065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_324861,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000142 DATDAA010000000
VERSION     DATDAA010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324861"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VIS73_04630"
     CDS             complement(<1..780)
                     /locus_tag="VIS73_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEY5762474.1"
                     /translation="MKAIVCRDAGGPEVMALAERPVPQPGPAEVLIRVAYAGVNRPDL
                     LQRAGRYAPPANASPHLGLEVSGVVVAKGDAVDLAVGSEVCALTPGGGYAEFVVVPAA
                     HCLPVPSGVSLRDAAGLPETCFTVWTNVFERARLQPGEVFLVHGGSSGIGVAAIQMAK
                     NWGARVFTTAGSAAKVAACAALGAEAIDYQAEDFVAAIKARNGGRGVDVILDMVGGDY
                     LPRNVAALAADGRLVSIAFLRGSRVEFDFMPVMLKRLTISGS"
     gene            917..1528
                     /locus_tag="VIS73_04635"
     CDS             917..1528
                     /locus_tag="VIS73_04635"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY5762475.1"
                     /translation="MSKPSTRFARSLLASLGATTLLAAAPAAAQETDENQTRHFYVTA
                     FAGAAHLNVGKLTGLVADSGYAAVGAAFGIRLADSLFLEIGHRRIGDGKDDEELELGR
                     NTYFKLDSGHIGPVWQLPLGENAEFTARCAGRRWRFIERTIPIGGPAQPDVVMRDSGA
                     GAYGRLGVAWRLNPAMTLGGGWTYLDLDGVGANTLEARFSYRF"
     gene            complement(2159..2234)
                     /locus_tag="VIS73_04640"
     tRNA            complement(2159..2234)
                     /locus_tag="VIS73_04640"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2199..2201),aa:His,seq:gtg)
     gene            complement(2316..2392)
                     /locus_tag="VIS73_04645"
     tRNA            complement(2316..2392)
                     /locus_tag="VIS73_04645"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2356..2358),aa:Arg,seq:tct)
     gene            complement(2432..2508)
                     /locus_tag="VIS73_04650"
     tRNA            complement(2432..2508)
                     /locus_tag="VIS73_04650"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2472..2474),aa:Pro,seq:tgg)
     gene            complement(2597..>3065)
                     /locus_tag="VIS73_04655"
     CDS             complement(2597..>3065)
                     /locus_tag="VIS73_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5762476.1"
                     /translation="LIALPCTQWRHTVVVPRGHPLADEPLTLQRLATFPLVTYDPAFA
                     GRSRIDEAFHRAGLAPNVALEAADADIIKTYVALGLGAGIIAGPAYDPERDRELVALD
                     AAHLFAANITKIGLRRGRFLRGYVYDFIELFAPQLPRSAVELALAGGGEEYQL"
BASE COUNT          567 a         1063 c          936 g          499 t
ORIGIN      
        1 cgaaccggaa atcgtcagcc gcttgagcat caccggcatg aaatcgaact cgacgcgcga
       61 acccctgagg aaggcgatgc tgaccaatcg gccatcggca gcgagcgccg cgacattgcg
      121 cggcaggtag tcgccgccga ccatgtcgag aatcacgtcg acgccacgac caccgttgcg
      181 cgccttgatc gcggcaacga agtcctcggc ctggtagtcg attgcctcgg caccaagcgc
      241 ggcacaggca gctactttgg cggcgctgcc tgccgtcgtg aagacgcggg cgccccagtt
      301 cttcgccatc tggatcgccg ccacaccgat gccgcttgat ccgccgtgca ccaggaagac
      361 ctcccccggc tgcaggcgcg cccgctcgaa tacattggtc cagaccgtaa agcaggtttc
      421 cggcagcccc gccgcgtcgc gcagcgaaac cccggacgga accggtaggc agtgcgctgc
      481 cggcacaacc acgaattcgg cgtagccgcc acccggtgtc agtgcgcaga cttcgctgcc
      541 gaccgccaga tccacggcat cgcccttggc taccaccacg cccgagacct caaggcctag
      601 gtgcggcgag gcattcgccg gcggcgcgta ccggccggcg cgctgcagga ggtcgggccg
      661 gttcacgccg gcataggcga cgcgaatcag cacctcggcc ggccccggct gcggcaccgg
      721 ccgctcggcc agggccatga cctcggggcc gccagcatcg cggcaaacaa tcgctttcat
      781 ggatttcgca acaggattca ttggagctgg atcaaacctt cctcgggtcg ggcgaccgtc
      841 gcgccgcgcc cagactaaac ttaccagcag taccgcccag tcaggcgggg catcctacca
      901 tcggaggtaa gccaccatgt ccaagcccag cactcgtttt gcccgcagcc tgctcgcctc
      961 gctcggcgcc accacgctcc tcgccgcggc cccggcggca gcgcaggaga ccgacgagaa
     1021 ccaaacccgt catttctacg tcaccgcctt cgccggcgcg gctcacctga acgtcggcaa
     1081 gctcaccggc ctcgtagccg actccggcta cgctgctgtc ggcgctgcct tcggcatccg
     1141 cctcgccgac agcctgttcc tcgaaatcgg tcatcgccgc atcggcgacg gcaaggacga
     1201 cgaggaactc gaactcggcc gcaacaccta cttcaagctc gacagcggcc acatcggccc
     1261 ggtctggcaa ctgccgctcg gcgaaaacgc cgagttcacc gccaggtgtg caggccggcg
     1321 ctggcgtttc atcgaaagaa ccatcccgat cggcgggccc gcgcaaccgg acgtcgtcat
     1381 gcgtgactcc ggcgccggag cctacggccg cctgggcgtc gcctggcgtc tcaacccggc
     1441 gatgaccctg ggcggcggct ggacctacct cgacctcgat ggagtcggcg ccaatacact
     1501 ggaggcgcgc ttcagctacc gcttctaggc ggcctacggc agatttgaga atcgaccagc
     1561 cgcctcatcg gcagctacct taacgtggtg agtttccaat cttggacctg ccccctttgc
     1621 tgccgcatca attccaccgc ttaggcccgc agtctgcggt tccctatcag ctgcgcaacc
     1681 cgtcaggcgc tactcgtgcc gatctgctcg cgcatctgga acggagcgac gcaaggcgct
     1741 agaacgaggc gcacaatcgc ccgctacgca acgccgcgcg gcagtccatt gcggtgctat
     1801 tcgggcgcat ctgctaacgc gcagccgcat agccggcacc gtgtgatcgc agtcaagaaa
     1861 agccgcccga aggcggctaa cgtatttcta cgaggaggag actgcgagtt cagactaggc
     1921 cggtctggta gccaccgcat taggcaatag tcatagatgc ggaactattc cctgagattg
     1981 ccgcggctat ccgaatgccg gtggcctccc cctaggatgc tgtcggcaac agcgattcgt
     2041 cagtggcacg gcaaatcagg acgaaaaaaa gggccggctt acccgaccct tcatgcttac
     2101 ccggcgctta cccaacttgc ttttgttagt gactactacc gccgaatatc ttgttttatg
     2161 gggtgaccga tgggactcga acccacaacc actggaatca caatccaggg atctaccaat
     2221 tgatctacgg ccaccgtcgt ttgctgcgac tgcacctacc actgacttcc tgacccaagg
     2281 tcgcgtttcg cgctgccgat gcgaccgact tctgttggca cgcccggcga gactcgaact
     2341 cacaaccctc ggcttagaag gccgatgctc tatccggttg agctacgggc gcccgagcat
     2401 cgcgccggca gcacggtaat gctggctgcc ttggtcgggg cgactggatt cgaaccagcg
     2461 accctctgct cccaaagcag atgcgctacc agactgcgct acgccccgag gagcgcgaat
     2521 tctaccgatg gccccatggc cggtcaatgc gttgctgcgt tgaaacgcgc agcggcgacc
     2581 gaccgtcgtg gcgtgcctac agctggtact cctcgccccc gccggccaac gccagctcga
     2641 ccgcggatcg cggcaactgc ggcgcgaaca gttcgatgaa gtcgtagacg tagccgcgca
     2701 ggaagcggcc gcggcgcagg ccgatcttgg tgatgttggc ggcgaacagg tgcgctgcat
     2761 cgagcgcgac gagttcgcgg tcgcgctcgg ggtcgtaggc cggcccggcg atgatgccgg
     2821 ccccgaggcc gagcgcgaca taggtcttga taatgtcggc gtcggcggct tcgagcgcga
     2881 cgttcggtgc cagcccggcg cgatgaaagg cctcgtcgat gcgcgagcgg cctgcaaacg
     2941 ccgggtcgta agtcaccagc ggaaaagtgg caaggcgctg cagcgtcagc ggctcgtcgg
     3001 ccagcggatg gccgcgcggc acgaccacgg tatgccgcca ttgtgtacag ggcagcgcga
     3061 tcagt
//
LOCUS       DATDAA010000143         2159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_499944,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000143 DATDAA010000000
VERSION     DATDAA010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499944"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..857)
                     /gene="argB"
                     /locus_tag="VIS73_04660"
     CDS             complement(45..857)
                     /gene="argB"
                     /locus_tag="VIS73_04660"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY5762477.1"
                     /translation="MTIVIKYGGHAMVDETLKSDFARDITLLKFIGLNPVVVHGGGPQ
                     ISQVLERMGIQSTFVRGMRLTDAATMDVVEMVLGGKVNKAIVAQINQQGGRAVGLSGK
                     DGGLLEARKLQIVVRADASQPPEIIDPGLVGEVTKVNAEIIETLAGKGFIPIIAPVGV
                     GPAGETFNINADLVASRIAMALGAGRLIYLTDVDGILDPAGQLISTIDAAGVERMVAD
                     GSIAGGMIPKIECALEALRSGVTKVPIINGRRRHSLLLELFTDKGIGTEVTL"
     gene            complement(963..>2159)
                     /gene="hslU"
                     /locus_tag="VIS73_04665"
     CDS             complement(963..>2159)
                     /gene="hslU"
                     /locus_tag="VIS73_04665"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEY5762478.1"
                     /translation="GPTGVGKTEIARRLSNLTDSPFSKVEASKFTEVGYVGRDVESMV
                     RDLVELTVNDMKQRAQEAVRGKARQMAEERLLDLLLPPSVPTTTPAGASPPAPEGEGG
                     AAANAGTREKLRRLLHAGKLDGRSVDLDVAERSTPMVEIFSSMGMEEMGINFKDLLGN
                     LLPKTTRRHRVKVPEALERLEQEAAQGLVDMERVVREAIAKVEQAGIIFLDEIDKIAG
                     KNGGHGPDVSREGVQRDLLPIVEGSTVTTKYGPVRSDHILFIASGAFHTVKPSDLIPE
                     LQGRFPIRVELDALGRDEFARILVEPRNALMLQYIALLRTEGVDLIFEDEAVTDIARI
                     AEEVNSRTENIGARRLHTIMECLLEDVLFAAPDTAVRRVVVDSAYVANKLQAITQDED
                     LSRYIL"
BASE COUNT          326 a          721 c          699 g          413 t
ORIGIN      
        1 tgtccagtcc gttgaaggtt gcttgggccc ccggcagcgc aacgctacag ggtgacttcg
       61 gttccgatgc ccttgtcggt gaagagctcc agcagcaggg aatgccgtcg gcggccgttg
      121 atgatcggca ccttggtgac gccgctgcgc aacgcttcga gggcgcactc gattttgggt
      181 atcattccgc cggcgatgct gccgtcggcg accatgcgct cgacacccgc cgcgtcgatg
      241 gtcgaaatca gctgcccggc agggtccagg ataccgtcga cgtcggtcag gtagatcagt
      301 cgtccggcgc cgagcgccat ggcgatgcgg ctggcgacca ggtcggcatt gatgttgaag
      361 gtctcgccgg ccggcccgac gcccaccggg gcgatgatcg ggatgaagcc cttgccggcc
      421 agcgtttcga tgatctccgc gttgaccttc gtcacctccc cgaccaggcc cgggtcgatg
      481 atctccggcg gctgcgacgc gtccgcgcgc accacgatct gcagtttgcg tgcctcgagc
      541 aggccgccgt ccttgccgct caggccgacc gctcggccgc cttgctggtt gatctgcgcg
      601 acgatggcct tgttgacctt gccgcccagg accatttcga cgacgtccat ggtcgcggcg
      661 tcggtcaaac gcatgccccg cacgaaggtc gactggatgc ccatgcgctc gaggacctga
      721 ctgatctgcg gaccgccccc gtggaccacg accgggttga ggccgatgaa cttcagcagg
      781 gtgatgtcgc gggcgaaatc gcttttcagc gtttcgtcca ccatggcgtg gccgccgtac
      841 ttgatgacga tcgtcatacc gtaaaaacgg cggatgtacg gcaaggcctc gatcaggatt
      901 ccagccgcat gttcgttctg catggttgcc tttcttggga ccgccggccg ccgcccgcgg
      961 gtctacagga tgtagcggct caggtcctcg tcctgggtga tggcctgcaa cttgttcgcg
     1021 acataggcac tgtcgacgac cacgcgccgt accgcggtgt cgggggccgc gaaaaggacg
     1081 tcctcgagca ggcactccat gatcgtgtgc aaccgccgag cgccgatgtt ttccgtgcgg
     1141 ctgttaacct cttcggcgat tcgtgcgatg tccgtcacgg cctcgtcctc gaaaatcagg
     1201 tcgacgcctt cggtacgcag cagggcgatg tactgcaaca tcagggcatt gcgcggctcg
     1261 accagaatgc gcgcgaactc gtcgcggccg agggcgtcga gttcgacacg gatcgggaag
     1321 cggccctgca gttcgggaat caggtcggac ggcttgaccg tgtggaaggc gcccgaggcg
     1381 atgaacagga tgtggtcgct tctgaccggc ccgtacttgg tcgtcaccgt cgaaccctcc
     1441 acgatcggga gcaggtcgcg ctgcacgccc tcgcgcgaga cgtccggccc gtggccgccg
     1501 ttcttgccgg cgatcttgtc gatttcgtcc agaaaaatga tgccggcctg ctcgaccttt
     1561 gcgatcgcct cccgcaccac ccgttccatg tcgaccagtc cctgggcggc ctcctgctcc
     1621 aggcgctcga gggcctccgg caccttgacg cggtggcgcc gggtcgtctt gggaagcagg
     1681 ttgccgagca ggtccttgaa attgatcccc atctcctcca tacccatgct cgaaaagatt
     1741 tcgaccatcg gcgtgctgcg ctcggcgacg tcgaggtcca cgctgcgccc gtcgagtttc
     1801 ccggcgtgca gcaaccggcg cagtttttca cgggtcccgg cgttcgccgc cgcgccgccc
     1861 tcgccctccg gcgcgggcgg ggacgcgcct gcgggcgtcg tcgtcgggac ggaaggcggc
     1921 agcagcaggt cgagcaaacg ctcctcggcc atttgccggg ctttgccgcg gaccgcctcc
     1981 tgggcgcgct gcttcatgtc gttgacggtc aactcgacca ggtcgcgcac catcgattcc
     2041 acgtcgcggc cgacgtagcc gacctccgtg aatttggaag cctcgacctt gctgaagggc
     2101 gaatcggtca ggttcgacag ccgccgcgca atctcggttt tgcccacacc ggtgggccc
//
LOCUS       DATDAA010000144         2429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_325015,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000144 DATDAA010000000
VERSION     DATDAA010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325015"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VIS73_04670"
     CDS             complement(<1..300)
                     /locus_tag="VIS73_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY5762479.1"
                     /translation="MSVYTTVSETALQEWLARYRLGPLESFVGIAGGVENTNYFVDTA
                     SGRYVLTLFERLDAKAAGRYLDLMAHLSTARVSCPGPVSDREGRRHGMLCGKPAAL"
     gene            complement(305..637)
                     /locus_tag="VIS73_04675"
     CDS             complement(305..637)
                     /locus_tag="VIS73_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HEY5762480.1"
                     /translation="MRITLILAITLLATAAAAQTGKPQPLPDLAPPLPEMKALDDALA
                     PQVTIVQRGEDRVEEFRIKGRLYMIKVTPPHGVPYYLIDERGDGRMIRHDSIDERLVV
                     PMWMILQF"
     gene            complement(646..1374)
                     /locus_tag="VIS73_04680"
     CDS             complement(646..1374)
                     /locus_tag="VIS73_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY5762481.1"
                     /translation="MSEKSKLPTLMRDESALSTPFAREAWRVFGIMSEFVEATDRLSA
                     VRPAVSIFGSARVQPEHRYYHLTERVARLLSDAGFSVISGGGPGIMEAANKGAFFGRS
                     PSIGLNIQLPHEQQSNTFQDISQTFRHFFARKYMFVRFASAYVVMPGGFGTLDELLEA
                     LTLIQTGKSRKIPLILMHRPYWAGMLDWFRDQLVREGMIDPGDMDLLQVIDEPEAVVE
                     AIFQHYETRGFDPLPDEKDLLLNL"
     gene            1397..>2429
                     /locus_tag="VIS73_04685"
     CDS             1397..>2429
                     /locus_tag="VIS73_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY5762482.1"
                     /translation="MSAGTLLLVDGSSYLYRAYHALPDLRSPAGLPTGAVYGVLSMLR
                     RMPGDFKATHCACVFDAKGKTFRDDWYADYKANRPPMPDDLVRQIEPLHEAVRALGWP
                     LLCETGVEADDVIGTLARQAAAAGFEVIISTGDKDLAQLVTPQITLINTMTNERLDVA
                     GVQQKFGVPPERIVDYLSLIGDSVDNVPGVDKVGPKTAVKWLDAYGSLDAIVAAADEI
                     GGKVGENLRAARDFLPLGRKLVTVVCDLDLPVRPEQLAVGEPDVAALRTIYEAQGFRS
                     WLKALNDPEGAKGEAKPVAAAADAPAPAALDASGKHREAYATVLDEGAFANWMQRIEA
                     ADAVAFDSET"
BASE COUNT          432 a          770 c          814 g          413 t
ORIGIN      
        1 cagtgcagcc ggcttgccgc aaagcattcc gtgccgccgt ccctcgcggt cggaaaccgg
       61 ccccgggcaa ctgacgcgtg ccgtggatag atgtgccatc aggtcgaggt agcgtcctgc
      121 tgctttggcg tccaagcgct cgaacagcgt tagcacataa cggccactgg cggtatcgac
      181 gaagtagttg gtgttctcga cgccaccggc gatgccgaca aaggattcga gcgggccgag
      241 gcggtagcgg gccagccatt cctgcagcgc cgtctcggag acggtcgtat aaaccgacat
      301 caatctagaa ctgaagaatc atccacatcg gtaccactag ccgctcgtcg atcgaatcgt
      361 ggcggatcat gcgaccgtcg ccacgttcgt cgatcaggta gtagggcacg ccgtgcggtg
      421 gtgtcacctt gatcatgtag aggcggccct tgatgcggaa ttcctcgacc cggtcttcgc
      481 cgcgctggac gatcgtcacc tgcggcgcga gcgcgtcgtc gagcgccttc atttcgggca
      541 gcggcggcgc caggtcgggc agtggctggg gcttgccggt ctgggcggcg gcggcagtcg
      601 cgagcagggt gatggcaagg ataagcgtga tacgcatggc gggcctcaga gattaagcaa
      661 cagatccttt tcgtcgggca gcgggtcgaa gccgcgcgtc tcgtagtgct ggaagatcgc
      721 ctcgaccacg gcctcgggct cgtcgatgac ctgcagcaga tccatgtctc cggggtcgat
      781 catcccttcg cgcactagct ggtcgcggaa ccagtcgagc atgccggccc agtacggccg
      841 gtgcatgagg atcagcggaa tcttgcggct cttgccggtc tggatcagcg tcagcgcctc
      901 gagcagttcg tctagcgtgc cgaagccgcc gggcatcacc acgtaggcgc tggcgaagcg
      961 cacgaacatg tacttgcgcg cgaagaagtg gcggaaggtc tgcgagatgt cctgaaaggt
     1021 gttcgactgc tgctcgtgcg gcaactggat gttgaggccg atcgacgggc tgcgcccgaa
     1081 aaacgccccc ttgttggcgg cctccatgat cccgggaccg ccgcccgaga tcaccgaaaa
     1141 tccggcgtcg gagagcagcc gcgcgacgcg ttcggtgagg tggtagtaac ggtgctcggg
     1201 ttgcacgcgc gcgctgccga agatcgacac ggccgggcgc acggccgaca ggcggtcggt
     1261 ggcctcgacg aactctgaca taatcccgaa gacccgccac gcctcgcggg cgaagggcgt
     1321 cgacagcgcc gattcatcgc gcatcagcgt cggcaacttg cttttttcac tcatgaccga
     1381 tccggtaggg gaacagatgt cagcggggac tttgctgctg gtcgacggct cgtcttatct
     1441 ctatcgtgcc tatcacgcac tgcccgatct gcgcagcccc gcgggcctgc cgaccggtgc
     1501 cgtctacggc gtcctgtcga tgctacgccg catgccgggc gacttcaagg cgacgcattg
     1561 tgcctgcgtt ttcgacgcca agggcaagac cttccgcgac gactggtacg ctgactacaa
     1621 ggccaaccgc ccgccgatgc ccgacgatct ggtgcggcag atcgagcctt tgcacgaagc
     1681 ggtgcgcgcg ctcggctggc cgctgctctg cgaaaccggg gtcgaggccg acgacgttat
     1741 cggcacgctc gcccggcagg ccgccgcggc cggcttcgag gtaatcatct cgaccggcga
     1801 caaggacctc gcgcagctgg tgacgccaca gatcacgctg atcaacacga tgaccaacga
     1861 gcggctcgac gttgccggcg tgcagcagaa gttcggcgtg ccgcccgagc gcatcgtcga
     1921 ctacctgagc ctgatcggtg acagcgtcga caatgtgccc ggcgtcgata aggtcggccc
     1981 gaagaccgcc gtgaaatggc ttgacgctta tggctcgctc gatgccatag ttgcagccgc
     2041 cgacgagatc ggcggcaagg tcggggagaa cctgcgtgcc gcgcgcgact tcctgccact
     2101 cgggcgcaag ctggtcaccg tggtctgcga tctcgacctg ccggtcaggc cagagcaact
     2161 ggcggtcggc gaacccgacg tcgcggcgct gcggacgatc tacgaggcgc agggctttcg
     2221 cagctggctg aaggccttga acgatcccga gggggcgaaa ggcgaagcga agccggtggc
     2281 tgccgccgct gatgcgcccg cgccggcagc gctcgatgcc tcgggaaagc atcgcgaagc
     2341 ctacgcaacc gtgctcgatg aaggcgcttt cgccaactgg atgcaacgca tcgaggccgc
     2401 cgacgctgtc gccttcgaca gcgagacga
//
LOCUS       DATDAA010000145         6684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_45422,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000145 DATDAA010000000
VERSION     DATDAA010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45422"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="VIS73_04690"
     CDS             <1..1241
                     /locus_tag="VIS73_04690"
                     /inference="COORDINATES: protein motif:HMM:NF014126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEY5762483.1"
                     /translation="SLGLDSGIRRVSELNMILAILLLVFVLVAGPTVFLLQTMVQNTG
                     MYISNLFDMTFNLYAYEPSGWIGGWTLFYWAWWVAWSPFVGMFIARVSRGRTIREFVV
                     GVLLVPVGFTFLWMTFFGDTALHMVMVQGLTDLSDAVAADTSVALFRFFEHLPLSGIM
                     SLLATLLVITFFVTSSDSGSLVVDMLTNGGHDNSPLWQRIFWAVLEGVVAAALLIAGG
                     LGALQTATIAAALPFSIVMILMCWGLVRALRIEMFKRISLRDARITPRGPHAAMGWQK
                     RLANIFHQPKREEVRGFLTGTVKPALAEVSEELTRQGRVARIGSDAQDTDRIWVEVGH
                     GEEIDFFYSVRPRAYEPPSFVITDTVASRAEALKYYRAEVHLREGSQDYDIMGWSKSD
                     IINDVLDHYERHMHFLQAVR"
     gene            1335..2558
                     /locus_tag="VIS73_04695"
     CDS             1335..2558
                     /locus_tag="VIS73_04695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5762484.1"
                     /translation="MISHIVPISSLLGGMGILLAGSGLLGTLLGLRGTLAGFSDLTVG
                     IVMAAFFLGYVVGARFCPPIIARVGHARAFSAFAAASAAISLAYGLYESPYFWGVLRV
                     ANGITLVGVYMVIESWLNERVTDHRAQVFSAYMMVSLIALALGQYLLLPFGVDGLGSF
                     LLIGVLFCIGLIPIALTPVAQPIITQSISLPLKKLYKKAPVGVAGGLVSGMVIGSFWS
                     LAAVYGNRSALDTAGVANLFALAIIGGALLQWPIGKLSDSRDRRMVLVWVALGGALAC
                     VLMQFSRPLGIPLPVAGFFFGSFAFSLYGLAVAQTHDRFHSSEALEATKSLLFLHGAG
                     AVIGPLVAGSLMTIASTVIFPAVVAVMQLCLAAYTLVHLRSDPPVPIEDRSQFLPMSR
                     SSPVAMDLDPRSPSP"
     gene            complement(2657..2746)
                     /locus_tag="VIS73_04700"
     tRNA            complement(2657..2746)
                     /locus_tag="VIS73_04700"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2710..2712),aa:Ser,seq:cga)
     gene            2837..4231
                     /gene="mltF"
                     /locus_tag="VIS73_04705"
     CDS             2837..4231
                     /gene="mltF"
                     /locus_tag="VIS73_04705"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HEY5762485.1"
                     /translation="MRQALSCLLALILAGCSFDSTVDIHTRSDELVVALRELPTSYQP
                     DGLDNGRGGKGFEYDLLSMLAEHLGKPLRVIPLPSENDVLRYLRHRRSHLGAAWLSVP
                     VQHPDAYWASRSFFESQPVIVQNVDDRPVTRPEDLVDRVVAVRAGSPLANRLLSGRYA
                     LVGGAAIAERPDYTGLRLIRAVADGKVPVAAVELPQLDLARTFLPDVQATLTLGDPRE
                     IVWLMQAGFDPELQIQINLFLEAITTDGRLERIFDRYFGHVRRLNRYDTVAFITRIRT
                     VLPRFNELFWKAEASTGIDWRLIAALSYQESHWDPLATSKTGVRGIMMLTGDTADRMG
                     VKNRLDPTESIRGGAEYLALLRDGMPDSIVEPDRTFIAIAAYNVGMAHIYSARRLARL
                     KGLNPDLWHELRRVLPLLSRAEYYNTLPTGRARGGETVIMVENIRAFYDILRRNAPPY
                     ETFAEITARPPPQP"
     gene            complement(4252..5538)
                     /gene="serS"
                     /locus_tag="VIS73_04710"
     CDS             complement(4252..5538)
                     /gene="serS"
                     /locus_tag="VIS73_04710"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY5762486.1"
                     /translation="MLDIQLLRSQLDSVSQRLATRGKAFDFSQFSALEAERRTLQNRT
                     QELQAKRNALSKEIGMKRGKGEDAAALMAEVNSLADELKASETRLAALLGELNAIVSA
                     IPNLPQASVPVGADAEANVEVKRWGSPRDFGFAVRDHVDLGTALGGLDFETAAKISGA
                     RFTLMKGQMARLHRALAQFMLDVHTAEHGYTEVYAPYLVNAESAFGTGQLPKFGEDLF
                     ATSRGDDDRLYLIPTAEVPVTNIVRDTIQQAEALPLKYVCHTPCFRSEAGAAGKDTRG
                     MIRQHQFDKVELVQIVRPEDSDAALEALTGHAETILERLGLPYRRMLLCTGDMGFSAA
                     KTYDLEVWLPAQSTYREISSCSSFEAFQARRMAARYRAGEKAKPELVHTLNGSGLAVG
                     RTLVAIIENGQQADGSVALPEALWPYMGGIEVLRAA"
     gene            complement(5541..>6684)
                     /locus_tag="VIS73_04715"
     CDS             complement(5541..>6684)
                     /locus_tag="VIS73_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY5762487.1"
                     /translation="VGKTTLARLMAQAFDADFVALSAVFSGVKDIREAVARAEMLRDQ
                     SGRRTILFVDEVHRFNKAQQDAFLPFVESGLFTFVGATTENPSFEVNAALLSRAAVYV
                     LKALDEAETSELLDRALAEPSVAIVIDATVRERLVGLADGDARRLINLVEQLASAARA
                     SGIDAIEGAFAERVLAQSLRRFDKGGDAFYDQISALHKAVRGSNPDAALYWLVRMLDG
                     GADPRYLARRIVRMAWEDIGLADPRAIQLANEAAATYERLGSPEGELALAQTVIYLAC
                     AAKSNAGYVAYNAARAFVASDGSRPVPMHLRNAPTKLMKDLGHGRDYRYAHDEDDGYA
                     AGERYLPDGMAEPGWYRPVNRGLEQKIAEKLAYLRARDRDANDRKD"
BASE COUNT         1107 a         2176 c         2060 g         1341 t
ORIGIN      
        1 tcagtttggg gctcgactcc ggaatccggc gcgtatccga attgaacatg attctcgcca
       61 tccttctgct ggtctttgtg ttggtggccg gaccgacagt gtttctcctg cagacgatgg
      121 tccaaaacac cgggatgtac atctcgaatc tgttcgacat gaccttcaac ctctacgcct
      181 acgagcccag cgggtggatc ggcggatgga cactgttcta ctgggcttgg tgggttgcgt
      241 ggtcgccttt tgtagggatg ttcatcgccc gcgtttcccg cggccgaacc attcgtgagt
      301 tcgtcgttgg cgtactgttg gtgccggtgg gctttacctt cctgtggatg accttcttcg
      361 gcgataccgc cctgcacatg gtgatggtgc aaggactgac cgatctctcg gatgccgtcg
      421 ccgcggatac ctcggtcgcc ttgttccgct tcttcgagca tttgccgctc tccggaatca
      481 tgtctttgct ggcaacactc ctggtgatta cgtttttcgt gacctcatcg gactcaggct
      541 ctctggtcgt cgacatgctg accaatggcg gccacgacaa ttcgccgctc tggcagcgga
      601 ttttctgggc tgtactcgaa ggcgtcgtcg ccgccgcact cctgattgcc ggtggactcg
      661 gtgcgctgca aaccgccacc atcgccgccg cgctgccatt ttccattgtg atgatcctca
      721 tgtgctgggg tctggtgcgc gcccttcgca tcgagatgtt caaacgcatt tcactgcgcg
      781 atgcccgcat cacgccacgc gggccgcatg ccgcaatggg atggcaaaaa cggctggcga
      841 acatctttca ccaacccaag cgagaggaag tccgcggctt tctcaccggc acggttaaac
      901 ccgcgcttgc cgaagtctcc gaagagttaa cgcgtcaggg acgggtcgcg cgcatcggtt
      961 ccgacgcgca ggacaccgac cgcatctggg tggaggtcgg tcacggcgaa gagatcgatt
     1021 tcttctattc ggttcgcccg cgcgcctatg aaccaccgtc ctttgtcatt accgacaccg
     1081 tggcttcacg cgcagaagcg ctgaagtact accgcgctga agtccatctg cgtgaaggct
     1141 cgcaggatta cgacatcatg gggtggagca agtcagacat catcaacgac gtgctcgacc
     1201 actatgaacg gcacatgcat tttctgcagg cggtgcgata agcatgccat cgccgctctg
     1261 gcagttggcg acaggcgcac gaacgtgctt gtcgtgtcgg tatgctaacg gccagcaggc
     1321 gccgagacaa gacagtgatt agccacatcg tgcccatctc cagcctactg ggcggaatgg
     1381 gcattctgct tgccggctcg ggcctgctcg gcaccctgct gggcctgcgc gggacgctgg
     1441 ccggattttc ggaccttaca gtcggcatcg tgatggcggc gttttttcta ggctatgtgg
     1501 tgggcgcacg cttctgcccg ccgataatcg cccgcgtcgg ccatgctcgc gccttctcgg
     1561 catttgccgc agcctctgcg gcgatttcat tagcttacgg cctctacgaa tcgccctatt
     1621 tctggggcgt gctgcgcgtc gccaacggca ttacgctggt cggggtctat atggtcatcg
     1681 agagctggct caacgagcgc gtcaccgacc accgcgccca ggttttttcg gcctacatga
     1741 tggtcagcct gatcgccttg gcactgggcc aatacctcct gctgcctttt ggcgtcgacg
     1801 gtctaggctc ttttcttctg atcggcgtac tgttttgcat cggcctgatt ccaattgcgc
     1861 tcactccggt cgcgcaaccc atcatcacgc aaagcatctc cctgccacta aagaagcttt
     1921 acaagaaagc gccggtgggt gtcgctggcg ggctggtatc cggcatggtc atcggctcgt
     1981 tctggtcact agcggccgtg tatggcaacc gtagcgccct ggacacagcc ggggtcgcca
     2041 acctgtttgc gctggcgatt atcggtggcg ccctgttgca atggccgatt ggcaaactct
     2101 cggatagtcg cgaccgacgc atggtgttgg tctgggttgc actcggcggc gcgctcgctt
     2161 gcgtgctgat gcagttctcc cgtcccttgg gcatccccct gccggtagcc ggattttttt
     2221 ttggcagctt tgccttttcc ttatatggcc tcgcggttgc ccaaacccac gaccggttcc
     2281 acagcagcga ggccctggaa gcaaccaagt ccctgctctt tctacatggc gcaggcgccg
     2341 tcatcgggcc gcttgttgca ggctccctga tgaccatcgc ctcaacggta atctttccgg
     2401 cagttgtcgc cgtcatgcaa ctctgtttgg ccgcctatac cttggtgcat ttgcgcagcg
     2461 acccgccggt gcccatcgaa gatcgcagcc agttcctgcc gatgagtcgc tcgtcccctg
     2521 tagcgatgga tctcgatcct cgctcgccct ctccctagcg tgcattgtgg cggacgccac
     2581 ctgcgctgac ttggttaggc cttatgggtt gtcgacaaga aaaaacgcgc cgaagggcgc
     2641 gttttctcac tgcaactggc ggagagggtg ggattcgaac ccacggtagg cttgcaccta
     2701 cgcctgattt cgagtcaggt acattcgacc actctgccac ctctccgcga agggtgcgca
     2761 ttgtagcacc ggtttctgtt ttgtcggcat ccgatgaggg tgccagaatg cgtgttctcg
     2821 ccttgcggaa taaccgatgc gacaagccct gagctgcctg ctggcgttga tcctcgccgg
     2881 gtgcagcttt gattccacgg tcgatatcca cacgcgcagc gatgagctgg tcgtcgccct
     2941 gcgcgaactg ccgaccagct accagccgga cggcctcgac aacggccgcg gcggcaaggg
     3001 cttcgaatac gatctgctga gcatgctcgc cgaacacctc ggcaaaccgc ttcgcgtcat
     3061 accgctgccc agcgaaaacg acgtgctgcg ctaccttagg caccggcgct cacatctcgg
     3121 cgccgcctgg ctgtcggttc cggtacagca tcccgatgcc tactgggcga gccgcagctt
     3181 cttcgagagc cagccagtga ttgtccagaa cgtcgacgac cgcccagtga cacgcccgga
     3241 agatctcgtc gaccgtgtcg ttgccgtgcg tgccggctca ccgctggcca accgcctgct
     3301 ctccggccgc tacgcgctgg taggcggcgc ggcgatcgcc gaacgcccgg actacaccgg
     3361 tctgcgactg attcgcgcgg tggccgacgg caaggttccg gtcgcagccg tcgaattgcc
     3421 acagctcgat ctggcgcgga cctttctgcc cgatgtccag gcaacgctta cgctcggcga
     3481 cccccgcgaa attgtctggc tgatgcaggc cggcttcgac cccgaactgc agatccagat
     3541 caacctcttc ctcgaggcaa ttaccaccga cggccggctg gaacgcatct tcgaccgcta
     3601 cttcggccac gtgcgtcggc tgaaccgtta cgacacggtc gcattcatca cccgaatccg
     3661 cacggtgctg ccgcgcttca acgaactgtt ctggaaggcc gaggcgagca ccggcatcga
     3721 ctggcgcctg atcgccgcgc tttcgtatca ggaatcgcac tgggacccgc tggcaacctc
     3781 gaagaccggg gtgcgcggca tcatgatgct gaccggcgac acggccgacc ggatgggtgt
     3841 taagaaccgg ctcgatccga ccgagtcgat ccgcggcggc gccgagtacc tcgcgctgct
     3901 gcgcgacggc atgcccgatt cgattgtcga acccgaccgt accttcatcg cgatcgctgc
     3961 ctacaacgtc ggcatggcgc atatctacag tgcgcgccga ctggcccggc tcaagggtct
     4021 caaccccgat ctctggcacg agctgcgacg cgttctgccg ctcctgtcgc gcgccgagta
     4081 ctacaacacg ctgccgaccg ggcgagcgcg cggcggcgag acggtgatca tggtcgagaa
     4141 catccgcgcc ttctacgaca tcctgcggcg caacgccccg ccctacgaga ccttcgccga
     4201 aatcacggcg cggccgccgc cccagccgta gccgggcgtc ggccggggat cctaggccgc
     4261 cctcagcacc tcgattcccc ccatgtaggg ccacagcgcc tcgggcaggg caacgctgcc
     4321 gtcggcctgc tggccgttct cgatgatcgc caccagcgtg cggccgacgg caaggccgga
     4381 accattgagc gtatgcacga gttcgggctt ggccttctcg ccggcgcgat agcgcgccgc
     4441 catgcgacgc gcctggaagg cctcgaagct cgaacacgac gaaatctcgc gataggtcga
     4501 ctgcgccgga agccagactt cgaggtcgta ggtcttcgcc gccgaaaagc ccatgtcgcc
     4561 ggtgcacagc aacatgcgcc gatacggcag gccgagccgt tcgagaatcg tctcggcgtg
     4621 gccggtcagt gcctcgagcg ccgcgtcgga atcctcgggc ctgacgatct gcaccagctc
     4681 gaccttgtcg aactggtgct gacggatcat gccgcgcgtg tccttgccgg cggcgccggc
     4741 ttccgaacga aagcacggcg tatggcagac gtatttcagc ggcagcgcct cggcctgctg
     4801 gatcgtgtcg cgcacgatgt tggtcaccgg cacctcggcg gtggggatca ggtagagccg
     4861 gtcgtcgtcg ccgcgcgagg tagcgaacag atcctcgcca aacttgggca actggccagt
     4921 gccgaatgcg ctctcggcgt tgaccagata gggagcgtag acctcagtat agccatgctc
     4981 ggccgtatgc acgtcgagca tgaactgcgc gagcgcgcgg tgcaggcgcg ccatctgccc
     5041 tttcatcagc gtgaaacgcg cgcctgatat cttcgccgcg gtttcgaaat cgagcccgcc
     5101 gagcgcagta ccgaggtcga cgtgatcgcg caccgcgaag ccgaaatcgc gtgggcttcc
     5161 ccagcgcttc acctcgacgt tggcctcggc gtcggcacca acgggaaccg atgcttgcgg
     5221 tagattggga atcgccgaga cgatcgcgtt gagttcgccg agcagcgccg ccagccgcgt
     5281 ctccgaagcc ttgagttcgt cggccagtga attgacctcg gccatcagtg ccgcagcgtc
     5341 ctcgcccttg ccgcgcttca tgccgatttc ctttgacagc gcgttgcgtt tcgcctgcag
     5401 ttcctgcgta cggttctgca gtgtccgtcg ctcggcttcg agcgccgaga actgcgagaa
     5461 gtcgaaggcc ttgccgcgcg tcgccagtcg ttgcgaaacg ctgtcgagct gcgagcggag
     5521 gagttggatg tcgagcatgg tcagtctttc ctgtcgttgg cgtcgcggtc gcgagcgcgc
     5581 aggtaggcga gtttttcggc gatcttctgt tcgaggcccc gattcaccgg acggtaccag
     5641 cccggctcag ccatgccgtc ggggagatag cgctcgccgg cggcgtagcc atcgtcctcg
     5701 tcgtgggcgt agcggtaatc gcggccgtgg ccgagatctt tcatcaattt cgtcggcgcg
     5761 ttgcgcaggt gcatcggcac cggccgcgaa ccgtcgctgg caacgaaggc gcgggccgca
     5821 ttataggcga cgtatccggc gttggatttc gccgcgcagg cgagatagat cacggtctgc
     5881 gccagcgcca gctcgccctc gggcgaaccg aggcgctcgt aggttgccgc cgcctcgttg
     5941 gcgagttgga tcgcgcgcgg atcggcaagg ccgatgtcct cccaggccat gcgcacgata
     6001 cgccgcgcca ggtagcgcgg gtcagcgccg ccgtcgagca tgcgcaccag ccagtacagc
     6061 gcggcgtccg ggtttgagcc gcgaaccgcc ttgtgtagcg ccgagatctg gtcatagaag
     6121 gcatcgccgc ccttgtcgaa gcggcgaagc gactgcgcca gcacgcgctc ggcgaacgcc
     6181 ccttcgatcg catcgatgcc gctcgcccgt gcggcgctgg cgagttgctc aacgaggttg
     6241 atgaggcgtc gcgcgtcgcc gtcggcaagc ccgaccagcc gctcgcgaac ggtcgcgtcg
     6301 atgacgatgg cgaccgaggg ctcggcgagc gcgcgatcaa gcagctcgct agtctcggcc
     6361 tcgtcgagcg ccttaagcac gtagacggcg gcgcgcgaga gcagcgctgc gttgacctcg
     6421 aacgacggat tttcggtagt cgccccgaca aaggtgaaca ggccgctctc gacgaaaggc
     6481 aggaaggcat cctgctgcgc tttattgaag cgatgtacct cgtcgacgaa aagtatcgtc
     6541 cgccgaccgc tctggtcgcg cagcatttcg gcgcgggcca ctgcctcgcg aatgtccttg
     6601 acgccggaaa agacggccga gagcgcgacg aagtcggcgt caaaggcctg tgccatgagc
     6661 cgcgccagcg tcgtcttgcc gacg
//
LOCUS       DATDAA010000146        10072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_360337,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000146 DATDAA010000000
VERSION     DATDAA010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10072
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360337"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1268)
                     /gene="hemA"
                     /locus_tag="VIS73_04720"
     CDS             complement(12..1268)
                     /gene="hemA"
                     /locus_tag="VIS73_04720"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY5762488.1"
                     /translation="MSIFAFGINHHSAPLAVREQVAFPEATLMRALTDLTQAEGVREA
                     AILSTCNRTELYCAAESPEQAVRWLSEHHKVALADLEPYLYTLEPREAVRHAFRVASG
                     LDSMVIGEPQILGQMKDAVRYAEEAGTLGALLSKMFHRSFEVAKEVRSSTAIGANIVS
                     MAAAAVHLSEQIFGSVSEQKVLFIGAGEMIELCATHFGARHPKQVTVANRTVERAMAL
                     AGRFNGNAVRLDTLGEILPQYDIIVSCTASPLPIIGLGMVERAVKARRHRPMFIVDLA
                     VPRDVEPEAGELDDVFLYSVDDLAQVVQSGMESRQAAVVEAEAIITSRVDGFVHWLET
                     RNTTVPTIRSLRDSAERMRRHELEHAMKLIARGEPVEKIVESLSQRLTNKFLHAPTQA
                     LNQADPEHRDELQQAAARLFHLHPGE"
     gene            complement(1371..2423)
                     /locus_tag="VIS73_04725"
     CDS             complement(1371..2423)
                     /locus_tag="VIS73_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5762489.1"
                     /translation="MKTRRLGGHAPTQAALDVSAVCLGTMTWGTQNSEADAHSQLDFA
                     VERGVNFIDTAEMYSVPPGAESYGKTETFIGSWLARQARDRVVLATKIAGGGRGMKWI
                     RGGELDFSEASLRRALEDSLRRLRTDYIDLYQLHWPDRNVPMFGETAFDPAAERESVP
                     IAETLAALDRLVQEGKIRAIGLSNETPWGLMRFLQVADALGLARVVTVQNAYSLLNRH
                     WEGGLAEIAFREEVALLPYSVLGFGHLSGKYLDDPQAAGRITQFPGFGQRYAKPNVAP
                     AVAEYVALARQHGLTPAQMAQAFVGSRWFVGSTIVGATSLAQLGENIAACEMTLSDEL
                     LAEIEAIHQRYPNPAP"
     gene            2563..2967
                     /locus_tag="VIS73_04730"
     CDS             2563..2967
                     /locus_tag="VIS73_04730"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY5762490.1"
                     /translation="MIVSSLREGARVETEIDGRFRIDVDQPAPYGADSAPSPFDVFLA
                     GIAACTAYYAQVYCRKWKLPHQGIRVALTPVFDKAHRLTDVSMHLHVPDNFPAEHRDG
                     LLRNAGNCLVKKTLESPPAIKLVLAVDAVVAA"
     gene            complement(3010..3085)
                     /locus_tag="VIS73_04735"
     tRNA            complement(3010..3085)
                     /locus_tag="VIS73_04735"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3050..3052),aa:Phe,seq:gaa)
     gene            complement(3234..5114)
                     /locus_tag="VIS73_04740"
     CDS             complement(3234..5114)
                     /locus_tag="VIS73_04740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEY5762491.1"
                     /translation="MLRRLLALACCLLPLPAVAQTLADEQRWLTFGAFLGIIAITLLI
                     THAAAQRVRTAADFYTANRGVSGMQNGWAIAGDYLSAASFLGVAGMISLWGFDGFFYS
                     VGWLVAYVSVLVVVAEPCRNLGKFTLTDILAYRNNPKATRIVGALSVITVSTFYLTAQ
                     MVGGGVLIKLLVGIDYDVSVILVGAVMLVYVLFGGMIATTWVQIVKAVLLVLASTILV
                     ILVWREYGFFGDFFANVVADPKIHSRVAQLIGDHAGAQDAVALGQRFLEPGLLLDAPL
                     DRISLCLALIFGTAGLPHILMRFFTVPTAREARRSVVWAMTIIGGFYVMSLFLGLAAA
                     MKVGAAEIAIIDAGGNMAAPLLAQYLGGGRDSLAGNLMLAFVAAVAFATILSVVAGLV
                     LATASAIAHDLYVGVIKKGHASSTEQIRAARISTLVVGTVAIAIGIAARGQNVAHLVA
                     LAFAVAASANFPSILLTLYWRRCNTAGVIAGMVVGTFTAIGLVLVGPGMTYPNVELAA
                     AHVVLDQEPARRARVVERLASPDLAEQESARRDALALDRAVAAARATSARYAAGGTSL
                     VGLSEPLFSLRNPGIVSIPIGFLAVIIASLLYRDPRAEEMWDELSVRQCTGMLTARPI
                     SG"
     gene            complement(5123..5452)
                     /locus_tag="VIS73_04745"
     CDS             complement(5123..5452)
                     /locus_tag="VIS73_04745"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEY5762492.1"
                     /translation="MSTRAQFDWTAVKADPLYRQYRQSRMRFVALLLGFSGLVFFPLP
                     LLAAFAPDFLAIRVHREINLGVLLVLAQFLASGLVAWAYTHRANRVFDPAAEQIFFHA
                     LDHYKKR"
     gene            complement(5544..6959)
                     /locus_tag="VIS73_04750"
     CDS             complement(5544..6959)
                     /locus_tag="VIS73_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5762493.1"
                     /translation="MLAPLLFVWPISIAITHYFATQVAGFPYDQALRDHVGAIVRLVR
                     IDNGVPQINLSTSARALLRSDEVDFVYFHVATRDGNRLIGDREFPFANDILSQPDEPG
                     TTHFRDAVIKGRDVRVAYQYLADPSITRDRWVLVEVAETLEKRNQLTNRIVASVILPQ
                     FIIIPLAIVLVWFGLSQGLKPLTRLRRRIERRPANDLSEIKSLGVPEEIGPLIAAFNA
                     MIARMQKNVAAQHRFIADAAHQLRTPLAGLKAQAQAARREPDPAVIERALENLVAGVD
                     RANRLVTQMLSLARAGGGDQALPMEDVTLDSLIRDIAAEWTPRMLDKGMDFGFETNAV
                     AVVTGNAFLLREMFNNLLDNALRYTPDGGRITARVTLHGDFVIVEIEDSGIGIPELDA
                     EQVFEPFYRADNAGPEGSGLGLAIVREIALLHRADVSLRPVAEGGGTVARVVFPVRED
                     EDRRASEVAAVVMRQQPAGFV"
     gene            complement(6997..7671)
                     /locus_tag="VIS73_04755"
     CDS             complement(6997..7671)
                     /locus_tag="VIS73_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5762494.1"
                     /translation="MRILLAEDDRLIADALSRSLRQTGYAVDVANNGADADAALIAGV
                     YDLLILDLGLPRLPGLEVLKRLRARNATLPVLILTALDGVDERVKGLDLGADDYMAKP
                     FEAAELEARVRALTRRSHGTTPTITCGKLTYDQAGRVAQIDGVSLDLSAREFGILEIL
                     LSRIGRLVSKDQLVDHLCGWGEEVSSNAIEVYIHRLRKKLEPGDVKIVTVRGLGYSLE
                     KPKSAA"
     gene            complement(7961..8884)
                     /gene="rarD"
                     /locus_tag="VIS73_04760"
     CDS             complement(7961..8884)
                     /gene="rarD"
                     /locus_tag="VIS73_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEY5762495.1"
                     /translation="MTAPAAADPTDATRDPHRGAYAAIAAYLIWGLTPVYFKALAAVG
                     AGEIIAHRVLWSVLLLAIVLLAARRRQGVEAIRRQPRLLLWLTLSAVLVTSNWLVYVW
                     AVNAGRVLEASLGYYINPLVTVVLGALVIGERMSRWQRIAFALAALGVANQIVQVGAL
                     PWVALFLATTFALYGLLRKQLAIDPLTGLFVETLLLAPLALWYLAGLSASGELAFGQL
                     GWRIDWLLVAAGLVTSLPLVLFAFGARRLRMATMGFLQYLAPTLMFGLGVLVYDEPFG
                     AGRLTTFALIWAGLAIYSWEAWRRPAGRHAG"
     gene            complement(9086..>10072)
                     /gene="groEL"
                     /locus_tag="VIS73_04765"
     CDS             complement(9086..>10072)
                     /gene="groEL"
                     /locus_tag="VIS73_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY5762496.1"
                     /translation="SNIRDLLPVLEQVAKAGRPLLIIAEDVDGEALATLVVNNIRGIL
                     KTVAVKAPGFGDRRKAMLEDIAVLTGGVVIAEETGLTLEKATLKDLGQAKRVEIGKEN
                     TILIDGAGEAKAIEARVKQIRIQIEEASSDYDREKLQERVAKLAGGVALIKVGAATEV
                     EMKEKKARVEDALHATRAAVEEGIVPGGGVALLRARANIKGLKGDNADQEAGIKIVLR
                     ALEQPLREIVANAGDEPSVVLAKVAEGKGNFGYNAANSTYGDLVDMGVLDPTKVERAA
                     LQNAASVAGLMLTTDCMVAESAKEDAPMGGGMGGGMGGMGGMGGMGGMGMDM"
BASE COUNT         1798 a         3315 c         3348 g         1611 t
ORIGIN      
        1 ggcaatcctg gctattcgcc aggatgcagg tgaaagaggc gcgccgctgc ctgctgcagt
       61 tcgtcccggt gttcggggtc agcctggttc agcgcctgcg ttggcgcgtg caggaatttg
      121 ttggtgagcc gctgcgacag cgattcgacg atcttctcga ccggttcgcc acgggcaatc
      181 agcttcatcg catgttcgag ctcgtgccgg cgcatgcgct cagccgagtc gcgcaggctg
      241 cggatcgtcg gcaccgtcgt gttgcgcgtt tccagccagt gcacgaaacc gtcgacgcga
      301 ctggtgatga ttgcctcggc ttcgaccacg gcggcctgcc gcgactccat gccgctttgc
      361 acgacctgag ccaggtcgtc caccgaatag agaaagacat catcgagttc gccggcttcg
      421 ggctcgacgt cgcgcggcac ggctaggtcg acgatgaaca tcggccggtg acgccgcgcc
      481 ttgaccgccc gctcgaccat gccgagtccg atgattggca gcgggctggc ggtacaagaa
      541 acgatgatgt cgtactgggg caggatttcg ccgagcgtat cgaggcgcac ggcattgccg
      601 ttgaagcgcc cggccagcgc catcgcccgt tcgaccgtgc ggttggcaac ggtcacctgt
      661 ttgggatggc gggcgccgaa gtgcgtcgcg cacagctcga tcatctcgcc ggcgccgata
      721 aaaagcacct tctgctcgct caccgagccg aatatctgct ccgagaggtg cacggcggcg
      781 gcggccatcg aaacgatgtt ggcaccgatc gccgtcgacg atcggacctc cttagccact
      841 tcgaaggaac ggtggaacat cttcgacagc aaggcgccga gcgtgccggc ctcctcggcg
      901 tagcgcacgg catccttcat ctggccgagg atctgcggct cgccgatgac catcgagtcg
      961 agcccgctgg ccacccggaa ggcgtggcgg acggcctcgc gcggttcgag ggtgtacaga
     1021 tagggttcaa gatcggcgag cgccaccttg tggtgctcgg acagccagcg caccgcctgt
     1081 tcgggcgact cggccgcaca gtacagctcg gtgcggttgc aggtcgacag gatcgccgcc
     1141 tcgcgcacgc cctcagcctg cgtcaggtca gtcagcgcac gcatcagcgt cgcctcgggg
     1201 aaggccacct gctcgcgcac ggcaagcggc gcggaatggt gattgatgcc gaaggcgaag
     1261 atggacatgg ggagaggcgg caaggacgga ctgacggcca aggggcggcg aattataccg
     1321 cccgcaggcc gccgcgccgc gcggcgcagg cctgacgcgg cgtttcctgg ctacggcgcc
     1381 gggttcggat aacgctggtg gatcgcctcg atttcggcga gcagctcgtc ggacagcgtc
     1441 atctcgcagg cggcaatgtt ctcgccgagt tgcgcaaggc tggtggcgcc gacgatggtc
     1501 gagccgacga accagcgcga gccaacaaag gcctgcgcca tctgcgccgg cgtcagtcca
     1561 tgctggcgcg ccagcgccac gtactcggcg accgctggcg cgacgttggg cttggcatag
     1621 cgctggccga agccgggaaa ctgcgtgatg cgaccggctg cctgcgggtc gtcgaggtac
     1681 ttgccgctga ggtggccgaa gccgagcacc gaatagggca gcagcgcgac ctcctcgcgg
     1741 aaggcgattt cggcgagccc gccctcccag tggcggttaa gcaggctgta ggcattctgc
     1801 acggtgacca cgcgcgccag accgagcgcg tcggcgacct gcaggaagcg catcaggccc
     1861 cagggtgtct cgttggaaag cccgatcgcg cggatcttgc cctcctgcac cagccggtct
     1921 agcgctgcca gcgtctcggc gatcggcacc gactcgcgct cggctgccgg gtcgaaggcg
     1981 gtctcgccga acatcggtac gttgcgatcg ggccagtgca actggtagag atcgatgtag
     2041 tcggtgcgca accgccgcag cgagtcttcg agcgcgcggc gcaggctggc ctcgctgaaa
     2101 tcgagctcgc cgccgcgaat ccacttcatg ccgcggccgc caccggcgat cttggtcgcc
     2161 agcacgacac ggtcgcgcgc ttgccgtgcc agccagctgc cgatgaaggt ctcggtcttg
     2221 ccgtagctct cggcgcctgg cggcaccgag tacatctcgg ccgtgtcgat gaagttgacg
     2281 ccgcgttcga cagcgaaatc gagctgtgaa tgggcatcgg cctcgctgtt ctgcgtgccc
     2341 caggtcatcg ttccaaggca gacggccgac acgtcgagcg cggcctgcgt cggcgcgtgg
     2401 ccgccgagtc gtcgggtttt catggggttt tccctctcat agcgccgtcc gattgtagcg
     2461 ccgtcaccgc tgcgggtcgg gccgaccagg tgaccgccgt caccgcagtc ggcgacccgg
     2521 ctgcgtatat tagtgttgca tgatcctcac cagaagccag ccatgatcgt ttccagcctg
     2581 cgcgaaggcg cccgcgtcga gaccgaaatc gacggacgct ttcgcatcga cgtcgaccag
     2641 ccggcaccat acggcgccga cagcgccccg agcccctttg atgtctttct cgccggcatc
     2701 gccgcttgta ccgcctacta cgcgcaggtg tactgccgca aatggaagct gccgcaccaa
     2761 ggcatccgcg tcgcactgac cccggtcttc gacaaggcgc accggctcac tgacgtgtca
     2821 atgcacctcc acgtgcccga caatttccct gccgaacacc gcgacggcct gctgcgcaac
     2881 gccggcaact gcctggtcaa gaagacgctg gaatcgccgc cggcgatcaa gcttgtgctc
     2941 gccgtcgatg ccgtagtcgc cgcatgattc tggcgcgatc gaccgaaccg aaaattgtct
     3001 gagggggagt ggtggccagg ggcggaatcg aaccgtcgac acgcggattt tcagtccgct
     3061 gctctaccaa ctgagctacc tggccacgag aactgctgat gcgacgaccg aacggccggc
     3121 gcaaaaaaga gcgcgcatta tagcgatgcc gccggggata ctcaactgaa aatcggcggc
     3181 aaccggcata gcaggcccga gcggcgggcc tggccgatgg aagcggtcgc caatcagccc
     3241 gaaatcggcc tcgcggtgag cattcccgtg cattggcgga cgctcagttc gtcccacatt
     3301 tcctctgcac gcgggtcgcg atagagcagc gaggcgataa tcacggcgag gaaaccgatc
     3361 ggaatcgaga cgatgcccgg attacgcagc gagaacagcg gctcgctcag gccgaccagc
     3421 gaggtaccgc cggcggcgta gcgcgccgag gttgcacgcg ccgcggcaac cgcccggtcg
     3481 agcgccagcg cgtcgcgacg ggccgactcc tgctcggcaa gatcgggact ggccagtcgc
     3541 tcgacgacgc gggcgcgacg cgccggctcc tgatcgagca ccacatgcgc tgcggcgagc
     3601 tcgacattgg gataggtcat tcccggtccg accagcacca gtccgatcgc ggtgaaggta
     3661 ccgaccacca tgccggcgat gacgcccgcc gtgttgcagc gccgccagta cagcgtcagc
     3721 aagatgctcg ggaaattcgc cgaagcggcg accgcgaagg ccagcgcgac caggtgcgcg
     3781 acgttctggc cgcgcgcggc gatgccgatg gcgatggcaa cggtgccgac gaccagcgtc
     3841 gagatgcgcg ccgcgcgaat ctgctcggtc gagctggcgt gccccttctt gatgacgccg
     3901 acgtagagat cgtgagcgat cgccgaggcg gtcgccagca ccagcccggc cactactgag
     3961 aggatggtgg cgaaggcgac ggccgcgacg aaggcgagca tcaggttgcc ggcgaggctg
     4021 tcacgcccgc cgccgaggta ctgggcgagc agcggtgcgg ccatgttgcc gccggcgtcg
     4081 atgatggcga tctcggcggc gccaaccttc atcgctgcag cgaggccgag gaacagcgac
     4141 atcacgtaga agccaccaat gatggtcatt gcccagacca ccgagcggcg tgcttcgcgc
     4201 gcggtcggca cggtgaaaaa acgcatcagg atgtgtggca gcccggccgt gccgaagatc
     4261 agtgccaggc acagcgaaat gcggtcgagc ggcgcgtcga gtaacagccc gggttcgagg
     4321 aagcgctggc cgagcgccac cgcatcctgg gcgcccgcgt ggtcgccgat cagttgtgcg
     4381 acgcggctgt gaatcttcgg gtcggcgacg acgttggcaa agaaatcgcc gaaaaatccg
     4441 tattcgcgcc agacgaggat gaccagaatc gtcgatgcca ggaccagcag cacggccttg
     4501 acgatctgca cccaggtcgt cgcgatcatg ccgccgaaca gcacgtagac cagcatcacc
     4561 gcgccgacga ggatcaccga aacgtcgtag tcgatgccca ccagcagctt gatcagcacg
     4621 ccgccgccga ccatctgcgc ggtcaggtag aaggtcgaca ccgtgatcac cgacagcgcg
     4681 ccgacgattc gcgtcgcctt cgggttgttg cggtaggcga ggatgtcggt gagcgtaaac
     4741 ttgccaaggt tgcggcaggg ttcggcaacg acgaccagaa ccgagacgta ggcgaccagc
     4801 cagccgaccg aatagaaaaa gccgtcgaaa ccccagagcg aaatcatccc ggcgacgccg
     4861 aggaaggacg ccgccgacag gtagtcgccg gcgatcgccc agccgttctg cattccgctg
     4921 acgccacggt tggcggtgta gaagtcggcc gccgtgcgca cacgctgcgc cgcggcgtgg
     4981 gtgatcagca gcgtgatcgc gatgatgccg aggaaggcgc cgaaggtcag ccagcgttgc
     5041 tcgtcggcga gcgtctgcgc cacggccggc aggggcagca ggcagcaggc cagggcaaga
     5101 aggcggcgca gcatcggctc gttcagcgct tcttgtagtg gtcgagcgcg tggaagaaga
     5161 tctgctcggc ggccggatcg aagacgcggt tggcgcgatg cgtataagcc caagccacca
     5221 gtccggaagc gaggaactgc gcgagcacga gcagcacgcc gagattgatc tcgcggtgca
     5281 cgcggatggc cagaaagtcg ggggcaaagg ccgcgagcag gggcagcgga aagaacacca
     5341 ggccggaaaa accgagcagc aaggcgacga aacgcatccg cgactgccgg tactgccggt
     5401 acagcgggtc agccttgact gcagtccagt cgaattgcgc gcgcgtactc atcgtcgatt
     5461 gaatcgcctc cggccgggaa catcaaggca aaagaatcta gacgccggtt cttacatcgg
     5521 gctgaccggc gttccggttg ggctcagacg aagccggccg gctgctggcg catgaccacg
     5581 gcggcgacct cgctggcgcg ccgatcctcg tcctcgcgga ccgggaagac gacccgcgcg
     5641 acggttcccc cgccctccgc caccgggcgc agcgagacat cggcccggtg cagcagggca
     5701 atctcgcgga cgatcgccag accgaggccg ctgccctcag gtccggcgtt gtcggcgcgg
     5761 tagaacggct cgaaaacctg ctcggcgtcg agctcgggga tgccgatgcc gctgtcctcg
     5821 atctcgacga tcacgaaatc gccatgcagc gtcacccgcg cggtgatccg cccgccgtcc
     5881 ggcgtgtagc gcagcgcgtt gtctagcagg ttgttgaaca tctcgcgcag caggaaggcg
     5941 ttgccggtga cgacggcgac ggcattggtc tcgaaaccga agtccatgcc cttgtcgagc
     6001 attcgcggcg tccactcggc ggcaatatca cgtatcagcg agtcgagcgt cacgtcctcc
     6061 atcggcagcg cctgatcgcc gccaccggcg cgcgccagcg agagcatctg cgtaaccagc
     6121 cggttggccc ggtcgactcc ggcaacgagg ttctccagcg cccgctcgat caccgccgga
     6181 tcgggctcgc gccgcgccgc ttgcgcctgc gccttaagcc cggcgagcgg cgtgcgcaac
     6241 tggtgcgcag cgtcggcgat gaagcggtgc tgggcggcga cgttcttttg catgcgcgcg
     6301 atcatcgcgt tgaaggcggc gatcagcggt ccgatttcct cgggaacgcc gagcgacttg
     6361 atctccgaga ggtcgttggc cggccgccgt tcgattcgcc ggcgcagccg cgtcaagggc
     6421 ttcagtccct gcgacaggcc gaaccagacg agaacgatcg ccagcgggat gatgatgaac
     6481 tgcggcagga tgacgctagc gacgatgcgg ttggtcagct gattgcgctt ttccagtgtc
     6541 tcggcaacct cgaccagcac ccagcgatcg cgcgtgatgc tcgggtcggc cagatactgg
     6601 taggcgacgc gtacgtcgcg gcccttgatg accgcatcgc ggaaatgcgt cgtgcccggc
     6661 tcgtctggct gcgaaaggat gtcattggcg aagggaaatt cgcggtcgcc gatcagccga
     6721 ttgccgtcgc gcgtggcgac gtggaaatac acgaaatcga cctcgtccga acgcagcagg
     6781 gcccgcgccg acgtcgagag attgatctgc ggcacaccgt tgtcgatgcg gaccaggcgg
     6841 acgatcgccc ccacgtggtc tcgcagcgcc tggtcgtagg ggaagccggc gacctgcgtc
     6901 gcgaaataat gggtgatggc gatgctgatc ggccagacga acaagagcgg cgcgagcatc
     6961 cagtcgagga tctcgccgaa cagcgaacgg tggccgtcag gccgcgctct tgggcttttc
     7021 cagactgtag ccaaggccgc gtacggtcac gattttgacg tcgccgggtt cgagtttctt
     7081 gcgcaggcga tggatgtaga cctcgatcgc gttgctgctc acctcctcgc cccaaccgca
     7141 caggtgatcg accaactggt ccttgctgac taggcgcccg atccgggaga gcaggatttc
     7201 gaggatgccg aactcgcgcg ccgacaggtc gagcgagacg ccgtcgatct gcgcgacgcg
     7261 gccggcctga tcgtaggtga gcttgccgca ggtgatggtc ggcgtcgttc cgtgcgagcg
     7321 gcgggtaagc gcccgcacgc gggcctcgag ttcggccgcc tcgaatggct tggccatgta
     7381 gtcgtcggcg cctaggtcaa gccccttgac ccgctcgtcg acgccatcga gcgcggtcag
     7441 aatcagtacc ggcaacgttg cgttgcgggc acgcaggcgc tttagcacct caaggccggg
     7501 cagccgcggc aggccgagat cgaggatcag caggtcgtag acgcccgcaa tcagcgccgc
     7561 gtcggcgtcg gcgccgttgt tggcgacatc gaccgcgtag cccgtctgcc tcaacgagcg
     7621 cgacagggca tcggcgatca ggcggtcgtc ctcggcgagg aggattctca tgtgcggccc
     7681 tccgccagga aaccgtagta agactgctgt aagcggccga tcctaatctg caatcgctgt
     7741 tctcctttgt ggtcttcgga gtgacgggta gccgacctct ggtcgtgccg cacggaactc
     7801 ggggagcgcc cgatctcctg caccgggcgt ctccctgcca ccttttttcc gcgccctcca
     7861 ccgcttgctg cctgccctcg ccggccctgt tgcgcctgca cggcgcgccg attctagcat
     7921 caagaatttc cccgtcacgc gtacgagagg tgcctgatgc ctacccggcg tgccggcctg
     7981 ccggccgccg ccaggcctcc caactgtaga tcgccagacc ggcccagatc agcgcaaagg
     8041 tcgtcagccg cccggctccg aaaggctcgt cgtagacgag tacgccaagc ccgaacatca
     8101 gcgtcggcgc gaggtactgc aggaaaccca tcgtcgccat ccgcaggcgg cgcgcgccaa
     8161 aggcgaagag cacgagcggc agcgaggtca ccaacccggc cgcaaccagc agccagtcga
     8221 tccgccagcc caactggccg aaagcgagtt cgccgctggc cgagagcccg gccaggtacc
     8281 agagcgcgag cggcgcaagc aacagcgtct cgacgaatag gccggtcagc gggtcgatcg
     8341 ccaactgctt gcggagcagc ccgtagagcg cgaatgtcgt cgccagaaac agcgctaccc
     8401 acggcagcgc gccgacctgg acgatctggt tggcgacgcc cagcgccgcg agcgcaaagg
     8461 caatccgctg ccatcggctc atccgttcgc caataaccag cgcccccagc accacggtaa
     8521 ccagcggatt gatgtagtag ccgaggctcg cttcgagcac gcggcccgca ttgaccgccc
     8581 agacgtagac cagccagttg ctggtcacca gcaccgccga cagggtcagc cagagcagca
     8641 ggcgtggctg gcggcggatc gcctcgactc cctgacgccg gcgcgctgcc agcagcacga
     8701 tggcgagcag caagaccgac cagagcacgc ggtgggcgat tatttcgcct gcaccaaccg
     8761 cggccaacgc cttgaagtag accggcgtca ggccccagat cagatacgcc gcgatggcgg
     8821 cataggcgcc gcggtggggg tcgcgcgtag cgtcggtcgg gtcggctgct gcaggcgcgg
     8881 tcatggcggt gctaggcgga atcggtgtcg gcaacggagc cggtgcttca aggccgcgaa
     8941 ttctagccgg ccccagagtc cggcgccgca ttgcacaaaa aggaaaaccc gccgaagcgg
     9001 gtttcctggt aaggctggcg caggactttg cgccggccgg atcgagaagc acgagcggct
     9061 gcccgcgcct tcgggccgac cggagttaca tgtccatgcc catgccgccc atgccgccca
     9121 tgccacccat gccacccatg ccaccaccca tgccgccgcc catcggggcg tcttccttgg
     9181 cggattcggc aaccatgcag tcggtggtga gcatcaggcc ggcaaccgaa gcggcgttct
     9241 gcagggcagc acgctcaacc ttggtcggat ccagcacgcc catatcaacc agatcgccgt
     9301 aggtgctgtt ggcagcgttg taaccgaagt tacccttgcc ctcggcgacc ttggccagca
     9361 cgaccgacgg ctcgtcaccg gcgttggcaa cgatttcgcg cagcggctgc tcgagagccc
     9421 gcagaacgat cttgatgccg gcttcctggt cggcgttgtc gcccttcagg cccttgatgt
     9481 tggcacgggc gcgcagcagc gcaacgccac cgccgggaac tatgccttct tcaacggcgg
     9541 cacgggttgc gtgcagcgcg tcttcgacac gggccttctt ctctttcatc tcgacttcag
     9601 tcgcggcacc gaccttgatc agtgcaacgc cgccggccag cttggcaacg cgctcctgca
     9661 gcttctcgcg gtcgtagtcg gacgaggcct cttcgatctg gatgcgaatc tgcttgaccc
     9721 gcgcttcgat cgccttggct tcgccggcgc cgtcgatgag gatggtgttt tccttgccga
     9781 tttcgacgcg cttggcctgg cccagatcct tcagcgtggc tttttcgagc gtcaggccgg
     9841 tttcctcggc gatgaccaca ccgccggtca gcacggcgat gtcttcgagc atggccttgc
     9901 gacgatcacc aaagcccggg gccttgacgg caacggtctt gaggatgccg cgaatgttgt
     9961 tgaccaccag ggtagccagc gcttcgccat cgacgtcttc ggcaatgatc agcagcggac
    10021 ggccagcctt ggcaacctgc tcgagtaccg gcagcagatc gcggatgttg ct
//
LOCUS       DATDAA010000147        13477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_10527,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000147 DATDAA010000000
VERSION     DATDAA010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13477
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10527"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1738
                     /gene="rnr"
                     /locus_tag="VIS73_04770"
     CDS             <1..1738
                     /gene="rnr"
                     /locus_tag="VIS73_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEY5762497.1"
                     /translation="ADPGMEIEIALRKHDLPHEFSPEALKQAKGIPDKVRKSDLEGRI
                     DLRELPLVTIDGETAKDFDDAVYCERKGKGWRLIVAIADVSHYLSPGTPLDREAYARG
                     NSVYFPRRVIPMLPEKLSNGICSLNPDVERLAMVCDMSISAQGEIGDYRFYPAVFRSH
                     ARLTYTQVWEWLSKAVAPTEAVHRALQPQLAELYALFQTLSKARGKRGAIDFETIESM
                     MVFDELGKISKIVPVIRNDAHRIIEECMLAANVCASEFLKAHEQTCLYRIHEGPTPEK
                     LKALRDFLKEFGFGLAGGDDPRAGDYAALLKQIQGRPDTQLLQTVMLRSLRQAMYSPD
                     NVGHFGLSYESYTHFTSPIRRYPDLLVHRAIKAVLSGANYKPGDWAEIGLHCSATERR
                     ADEATRDVETWLKCYFMRDRVGEEFSATIASVVAFGVFVSLDEVHIEGLVHVSDLGED
                     YFQFDAGRHQMLGERSGKRFRLGDRLRVQLVRADLDTGKIDFTLAAEASAVARKSRSG
                     VPSTPMFIDLPPAERPAASVSNKRSAGKAGASGEKGRSGRQAKGGDAGRSRRGAGSKP
                     AADRRKPSRRKD"
     gene            complement(1822..2676)
                     /gene="rlmJ"
                     /locus_tag="VIS73_04775"
     CDS             complement(1822..2676)
                     /gene="rlmJ"
                     /locus_tag="VIS73_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEY5762498.1"
                     /translation="MLSYRHAFHAGNHADVLKHLVLLQCLAQLNRKETPYWVVDTHAG
                     AGIYPLQSAVGEKLAEWRDGIARLWEASADDLPEPLLDYRQRVARLNTDGQLHNYPGS
                     PWFAWSLSRDGDSLRLFERHPADAQTLRGNLDGAGGRVRVAESDGFAGLKALLPPPPR
                     RGLVLIDPSYETRGDYDAVPLALAEGLKRFATGIYAIWYPRLSRRDARQLPERLKDVS
                     AARPWLDVTLDVTRPAADGHGLFGSGMFVVNPPWTLRTALDASLPWLAEHLGERGVYS
                     VTESQPTT"
     gene            2805..4952
                     /locus_tag="VIS73_04780"
     CDS             2805..4952
                     /locus_tag="VIS73_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5762499.1"
                     /translation="MNDDNLHGVLRNTVEAIAGLSGKHFFEQLVLTLSRSLGVRHALV
                     GEISEDGQSIATLAACSGDRTVPNFLYPLAGSPCASALDTATCLVSQRVQAAFPADAL
                     LAQMGAESYLGTPLVNQSGEKLGVLAVVDDSALPDDPSMRSLFELFAREAAGELERQR
                     LERELRLAAAVFDYTGEGILVCDADKCAVKINQAYTRITGFTIDEIRGQVPLIFQPER
                     YEASVYAAIWEAVERAGYWQGEVFDRRKSGEVYTQWLSISGIDDEGRGRQYVAVFSDL
                     SGLKQAQERLSFLVHHDPLTGLPNRTLMQQRVQFAISRARRKETRMALLYIDLDRFKV
                     VNDTLGHSVGDELLKSAARRLVRSLREVDVIARLGGDEFIVLIEDLVDAKAAAVVAEK
                     IQLQLAEPFMLQHQEVVTTSSIGISMYPADGSDLQTLLKNADMAMYQAKELGRNGYQF
                     FKAENNQRSLERLSLEAALRRAADRDELLLHYQPVVDVTDGRVVGVEALVRWRHPDLG
                     LVSPVQFIPLAEETGLIVTIGAWVLEEACRQAKAWHNQGYRDLTVAVNLSPRQFRQAG
                     LQEIVRNALWKSGLTPRHLELEITEGVLMQNVDEALHVLEAFDAMGIRLLMDDFGTGY
                     SSLSYLKRFPIHTLKIDRSFVSGLPGDQDDAALAGTIVAMAHALKLDVIAEGVEKVEQ
                     LEFLRDQRCERYQGFLFSPPRPAGEIEALLQKR"
     gene            complement(4971..5594)
                     /locus_tag="VIS73_04785"
     CDS             complement(4971..5594)
                     /locus_tag="VIS73_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEY5762500.1"
                     /translation="MAESGRARAGTSPHTPFEDAQAIATGTLLFAVAVLVFGKSGILT
                     GGTAGVTFLLHYASEVSFPVLYLLVNLPFYVFAKQALGWRFTIKTFAAVSLLALYVRL
                     IPAWFVIDEIDPLFGAILGGLLGGAGLLMLMRHQASLGGLGVLAIWLQDRHGWRAGKV
                     QMAVDCVIVSTAVLVIGVEAAVLSGLSALLLNLVLTINHKPGRYAGY"
     gene            complement(5599..7485)
                     /locus_tag="VIS73_04790"
     CDS             complement(5599..7485)
                     /locus_tag="VIS73_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEY5762501.1"
                     /translation="MSPAPIPLELLAPARNAEIGIAAIDHGADAVYIGGPGFGARSGA
                     DNTMSDIARLVEHAHRYHARVYLTLNTILRDDELEPARQLAWDAWNAGVDALIVQDMG
                     LLDLELPPIALHASTQMDNRSPAKVAFLEAVGFSQVVLARELSLTEIAAITATTTVPL
                     EYFIHGALCVSYSGQCSISHALTGRSANRGECAQICRLPFSLSDGETTIVADKHLLSL
                     KDNDQSANLAGLAEAGIRSFKIEGRLKDRAYVKNITAHYRLLLDRLIADDPRWCSASS
                     GHSRFGFTPDPGKTFRRGATDYFTHERQADIGAFDTPKAAGEPIGEVLAVGREAIDVA
                     PTPPLGNGDGIAFFYASGELAGLRVNRVEQLADRQRLHVLRGVLLPVAGTPLFRNRDH
                     AFDRQLEHDSATRQIEIDLVFSATDDGIRLQATDSDGIAASADLPGQFEAAIEPVREA
                     ERIRQQLGKLGSTIFTARRIEVASTTVPRLSAAALNGLRRAAIESLEAARRQRYARPR
                     RADARVPAPPYPETELSFLANVLNASARAFYIRHGVLKIADAYETNQERGEVSLMITR
                     HCLRYSFNLCPKEVKGIRPDPLTLENGGQKFTLRFDCKRCEMHVVGKLRQAGKGFPLP
                     VRAT"
     gene            7628..8581
                     /locus_tag="VIS73_04795"
     CDS             7628..8581
                     /locus_tag="VIS73_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762502.1"
                     /translation="MAAIAISLAPAPSIAQSVSPADEAAGAPPAYRAPLPPAKRGREI
                     ELIRELGAYYTSIAVQIPLADAPLAEGGDVGEMEVYRRLFEASASPSALLLEASVYPM
                     PLLGTHLKKHSPDFYDTRWLGVDIVPAVTAGFQEPWALAAFVGADMRFTRPGEAERDT
                     NRGYMGYLVSVGNRHIRDNVMIDDDWFELEWKMKGERIFVADRLTWSFRVGTRWHANA
                     DIRDTVYLGISRSDTNYGSPMLRWLNNTEVRLTTEFSQRDFDFLRQEIIFGKKIPLAH
                     WFSALQFDVGVIHEQRGKYTGQLGAQSAGGTTFIFRPGLEF"
     gene            complement(8600..9121)
                     /locus_tag="VIS73_04800"
     CDS             complement(8600..9121)
                     /locus_tag="VIS73_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY5762503.1"
                     /translation="MKRFIAAAVLAASSTTALAQASPQSGVYGYGQIGPADLQGVRDG
                     VAIKFGADFMKDLAGVKGLGLTGFVSRWDGDSRGTDITGTTFAGGATFDVPLPDTRFS
                     LQARAFLSYSRVEVDFAPFGGGFSDNSLDLGFGIGGAYRIDNRLSVRVDYDLISADGS
                     DADILTGGIGYRF"
     gene            complement(9271..9945)
                     /locus_tag="VIS73_04805"
     CDS             complement(9271..9945)
                     /locus_tag="VIS73_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762504.1"
                     /translation="MITSRKLLIVVPALLIVAAWIALRSAPPPRVNTLPPPWEVTVSP
                     DGSTSEVLGIRLGTTTLSEARQRFGGDLQLALIEQPDRQLALEGYAARVESAFVTGAV
                     VLAAAAPPSLLDAARQRAIQSAAQPSGARRWSLSPADFDAALAMHVDSLTFVPSIRLD
                     AAMVETRFGPAERTLLRGKRVHYLYPLQGIDVAVDESGKAVLQYLRPVDFARLAVPLD
                     AAEANP"
     gene            complement(10038..10589)
                     /locus_tag="VIS73_04810"
     CDS             complement(10038..10589)
                     /locus_tag="VIS73_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY5762505.1"
                     /translation="MTNQIPEQFAAANKAAVDALLTVANTALASAERVAALNLNTARA
                     ALSDGAANAKTLMGVKDVQDLVETQKSLAQPAVEKAVSYSQNVYAITTDTQQALAKLV
                     EAQFADFRKNVADLVAQASSKAPAGSEVAVAAMQSAFAAANSAFDNMNKVAKEVAEVA
                     EKNVAAATSATVKAMGTTGGKKK"
     gene            complement(10857..12134)
                     /gene="ppk2"
                     /locus_tag="VIS73_04815"
     CDS             complement(10857..12134)
                     /gene="ppk2"
                     /locus_tag="VIS73_04815"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEY5762506.1"
                     /translation="MAKTDTPKTEAADKATKQAGESAATGTLRRARKAVARKASSADK
                     AATKGKRPAAARPATAAIPRKDRAARNGKPTQRRTRSAAAGDVAPSTTPSGIENFETR
                     EAEDGARVRRIAAMNDVISGVPNEEAISLLKNLLKQRRIAADERDEHPDDVLARGWRE
                     GGYPYKNLMARRTYERQKYRLQVELLKLQAWVKESGQKVVVLFEGRDAAGKGGTIKRF
                     MENLNPRGARVVALEKPSEIERGQWYFQRYVEHLPTAGEIVMFDRSWYNRAGVERVMG
                     FCSDAEYHEFMRQVPEFERMMVRNGIHLVKFWFSVSREEQRRRFKERKVHPLKQWKLS
                     PIDLASLDKWEDYTKAKEAMFFYTDTADAPWTVIKSDCKKRARLNAMRYILHRLPYTN
                     KDVARIGALDTLIVGRANVVFERGENIGTPTDI"
     gene            complement(12164..>13477)
                     /gene="ettA"
                     /locus_tag="VIS73_04820"
     CDS             complement(12164..>13477)
                     /gene="ettA"
                     /locus_tag="VIS73_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY5762507.1"
                     /translation="FDKLAEEQARLEAIIASTGSDTEHQMEIAADALRLPPWDASIKT
                     LSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAVTHDRY
                     FLDNAAEWILELDRGEGIPWKGNYSSWLEQKEARLETESKQEGARMKAMKQELEWVRS
                     NPKARQAKSKARLARFDEMSSAEYQKRNETQEIFIPVAERLGGQVIEFKDVTKAFGDR
                     LLIDKLSFSIPPGAIVGIIGPNGAGKSTLFRMITGSEQPDSGEVVIGQTVKMAYVDQS
                     RENLSSEKTVFDELADGNDLITIGKFEMPSRAYIGRFNFKGADQQKIVGKLSGGERGR
                     LHLAKTLMTGGNLLLLDEPSNDLDVETLRALEDALLEFAGSALIISHDRWFLDRICTH
                     ILAAEGESQWSFFAGNYQEYEADKKKRLGEEGAKPKRIRYKPISR"
BASE COUNT         2288 a         4134 c         4558 g         2497 t
ORIGIN      
        1 cgccgatccg ggtatggaaa tcgagatcgc gctgcgcaag cacgatctgc cgcacgagtt
       61 ctcgcctgag gcgctgaagc aggcgaaggg gattcccgac aaggtcagaa aaagtgacct
      121 cgaaggccga atcgacctcc gcgagctgcc gctcgtaacg atagacggcg aaacggcgaa
      181 ggacttcgac gatgccgtct attgcgaacg caaggggaag ggctggcgac tgattgtggc
      241 gatcgccgac gtctcgcact acctctcgcc gggcactccg ctcgaccgcg aggcctacgc
      301 gcgcggcaac tcggtttact ttccgcggcg cgtcattccg atgctccccg agaagctgtc
      361 gaatggcatc tgctcgctca atcccgatgt cgagcggctg gcgatggtct gcgacatgag
      421 tatttcggct cagggcgaga tcggcgatta ccgcttctat cccgcggtct ttcgctcgca
      481 cgcgcgcttg acctataccc aggtctggga atggctgtcg aaggccgtcg cgccgacgga
      541 agctgtgcac cgcgcgctgc agccgcaact tgccgagctt tacgccttgt tccagacctt
      601 gtccaaggcg cgtggcaagc gcggcgcgat cgacttcgag accatcgagt cgatgatggt
      661 cttcgacgag ttggggaaga tctcgaagat cgtgccggtg atccgcaacg atgcgcaccg
      721 gatcatcgag gagtgcatgc tggccgccaa cgtatgcgcc tcggagtttc tcaaggccca
      781 cgagcagacc tgtctttatc gcattcacga aggtccgacg ccggagaagc ttaaggccct
      841 gcgcgacttt ctcaaggagt tcggtttcgg tctggccggc ggcgacgacc cgcgcgccgg
      901 cgattatgcg gcgctgctta agcagatcca ggggcggccc gacacgcagc tgctgcagac
      961 ggtcatgctc cgctcactgc ggcaggcgat gtacagcccg gacaacgtcg gccacttcgg
     1021 gctgtcgtac gagagctaca cgcacttcac ctcaccgatc cggcgctacc cggatctgct
     1081 cgtgcaccgg gcaatcaagg cggtgctctc cggcgcgaac tacaagcccg gcgactgggc
     1141 cgagatcggc ctgcactgct cggcaaccga gcggcgcgcc gacgaggcga cgcgcgatgt
     1201 cgagacctgg ctcaagtgct acttcatgcg cgatcgtgtc ggcgaagagt tctcggcgac
     1261 gatcgcctcg gtggttgcct tcggcgtttt cgtctcgctc gatgaggtcc acatcgaggg
     1321 actcgttcat gtctccgacc ttggcgagga ctacttccag ttcgacgctg gcaggcacca
     1381 gatgctcggc gagcggagtg gcaagcgttt ccgtctcggc gaccggctgc gcgttcagct
     1441 ggtccgcgcc gacctcgaca ccggcaagat cgacttcacg ctcgccgccg aagcgtccgc
     1501 cgtagcccgc aagtcgcgca gcggtgtgcc gagtacgccg atgttcatcg acctgccgcc
     1561 ggccgagcga ccagccgctt cggtgtcgaa caagcggagc gcgggcaagg caggggcatc
     1621 gggcgaaaag ggcagaagcg gtcgtcaggc gaagggtggc gatgccggcc ggagcagacg
     1681 cggagccggc agcaagccgg ccgcggaccg gcgcaagccc tcgcgccgca aggattgatc
     1741 tgctgccggc ggcgcgtcgc tcggacctgc agccgcttga ggggccgtcg tcgcgcaacc
     1801 gatgccggcc ggcgaccgtg atcaggtcgt gggctggctt tcggtaaccg aatacacgcc
     1861 ccgctcgccg agatgttccg ccagccatgg cagcgatgca tcgagtgcgg tgcgcagcgt
     1921 ccacggcgga ttgactacga acatgccgct gccgaacagg ccatggccgt cggcggccgg
     1981 gcgcgttacg tcaagcgtaa cgtcgagcca cgggcgcgcg gcggatacgt ccttgaggcg
     2041 ttccggcaac tggcgggcat cgcggcgaga cagccgtggg taccagatgg cgtagatgcc
     2101 agtggcgaaa cgcttgagtc cttcggcgag cgcgagcgga accgcgtcgt agtcgccgcg
     2161 cgtttcgtac gaggggtcaa tgagtacgag gccgcgccgc ggtggcggcg gcagcagagc
     2221 cttgaggcca gcgaagccat cgctttcggc gacccggacg cggccgccgg caccgtcaag
     2281 atttccgcgt agcgtctgcg catcggccgg gtggcgctcg aacagacgca gcgaatcgcc
     2341 gtcgcgcgac agcgaccagg cgaaccacgg cgaacccgga tagttgtgca gctgcccgtc
     2401 ggtgttgagt cgggcaacac gctggcggta atcgagcagc ggctctggca ggtcatcggc
     2461 cgacgcttcc cagaggcgag cgatgccgtc gcgccattcg gcgagctttt cgccgacggc
     2521 actctgcagc ggatagatgc cggcgccggc atgggtgtcg acgacccagt agggcgtctc
     2581 cttgcggttt agttgcgcca ggcactgcag caggaccagg tgcttgagga cgtcggcgtg
     2641 atttccggcg tggaaggcgt ggcggtagct gagcatgtag agcccgaatt gatgccgagc
     2701 gcggcattct acgggaggta ggcgcgggaa ggtctgctag catccgggcc ggcgtagtcg
     2761 aaagcggctg cggcgcttcg gaatcgtgtt cggggaaggc gagcgtgaac gacgacaatc
     2821 tgcatggtgt actgcgcaac acggtcgagg caattgcggg cctttccgga aagcattttt
     2881 ttgagcaatt ggtactgacg ctttctcgct cgctcggcgt gcgacatgcg ctggtcgggg
     2941 agatcagcga ggacggccag tcgattgcga cgctcgcggc ttgcagcggc gacaggaccg
     3001 tacccaactt tctctacccg ctagctggat cgccgtgcgc ctccgcgctc gacacggcaa
     3061 cctgtctcgt atcgcagcgc gtgcaagccg cgtttccagc cgatgccctg cttgcacaaa
     3121 tgggtgcaga aagctatctc gggacgccgc tggtcaacca gtcgggcgaa aagcttggcg
     3181 tgttggcggt cgttgacgac tcggcgctgc cggacgatcc atcgatgcgc tccctctttg
     3241 aattgtttgc tcgcgaagcg gccggggaac tcgagcgtca gcgcctggag cgcgaactgc
     3301 gactggcagc ggcagtcttt gactacaccg gcgagggaat cctcgtttgc gatgccgaca
     3361 agtgcgcggt caagattaat caggcctaca cacggataac cggtttcacg atcgatgaga
     3421 ttcgcggtca ggtgccgctg atattccaac ctgagcgcta cgaggcgagc gtctacgcgg
     3481 cgatctggga ggccgtcgag cgcgcaggct attggcaggg cgaggtcttt gaccggcgca
     3541 agtcgggcga ggtctatacc cagtggctgt cgatcagcgg catcgacgat gaaggccggg
     3601 gccgccagta cgttgcggtc ttctccgacc tctctggcct caagcaggcg caggagcggc
     3661 tgtctttcct ggtccaccac gatccgctga ccgggttgcc taaccggacg ttgatgcaac
     3721 agcgcgtcca gtttgcaatc agccgcgcgc gtcgcaagga aacgcggatg gcactgctct
     3781 acatcgacct cgatcgcttc aaggtcgtca acgacacact cggccattcg gtcggcgacg
     3841 aattgctgaa aagcgccgcc cggcggctgg tccgctcgct gcgagaagtc gacgtcattg
     3901 ctcgtctcgg cggcgatgag tttatcgtgc tgatcgagga cctagtcgat gccaaggcgg
     3961 ctgcggtcgt cgccgagaag atccagctgc agctcgctga acccttcatg ctgcagcacc
     4021 aagaagtcgt caccacctcg agcatcggca tcagcatgta tccggctgat ggcagcgatc
     4081 tgcagaccct gcttaagaat gccgacatgg cgatgtatca ggccaaggag ctcggtcgca
     4141 acggctacca gttcttcaag gccgagaaca atcagcgctc gcttgagcgg ctttcgctgg
     4201 aagcggcgtt gcgccgcgcc gcagaccgcg acgagttgct gctccactac cagccggtgg
     4261 tcgatgtgac tgacggccgt gtcgtcggcg tcgaggcgct ggtgcgctgg cgacatcccg
     4321 atctaggact cgtgtcgccg gtgcagttca ttccacttgc cgaggaaacg gggctgatcg
     4381 tcactatcgg cgcctgggtc ctcgaagagg cctgccggca ggccaaggcc tggcacaatc
     4441 agggttatcg cgacctgacc gtggcggtga acctctcgcc ccggcagttc cggcaggccg
     4501 gcctgcagga gatcgtgcgc aacgccttgt ggaaatctgg actgacgccg cgtcatctcg
     4561 aactcgagat caccgagggt gtgctgatgc agaacgtcga cgaggcgctg cacgtgctcg
     4621 aggctttcga cgctatgggc atccgcctgc tgatggacga tttcggcacc ggttattcga
     4681 gtctcagcta tctcaagcgc ttcccgatcc acacgctgaa gatcgatcgc tccttcgtca
     4741 gcggacttcc cggcgaccag gacgatgcgg cgctggccgg caccatcgtc gccatggccc
     4801 acgcgctcaa gctcgacgtg atcgccgaag gcgtggagaa agtcgagcag ctcgagttcc
     4861 tgcgcgacca gcgctgcgag cgctatcagg gattcctctt cagcccgccg agaccggctg
     4921 gggaaatcga ggcgctgctg cagaagcgtt gagtcggttg cggcggccgg tcaataaccg
     4981 gcgtagcgcc caggcttgtg gttgatcgtc agtaccagat tgagcagcag cgctgataga
     5041 cccgacagca cggcggcttc gacaccgatc actagcaccg cggtgctgac gatcacgcag
     5101 tcaaccgcca tctgcacctt gccggcacgc cagccgtggc ggtcctgtag ccagatcgcc
     5161 agcacgccaa gaccgccgag gctggcctga tgccgcatca gcatcagcag gccggcaccg
     5221 ccgagcaggc cgccgagaat tgcgccgaac agtggatcga tctcgtcgat gacgaaccat
     5281 gcgggaatca gcctaacgta gagcgcgagc aacgaaaccg cggcaaaggt cttgatcgtg
     5341 aagcgccagc cgagcgcttg cttggcgaag acgtagaagg gcaggttcac cagcaggtag
     5401 agcacgggga aggaaacctc gctcgcgtag tgcagcagaa aggtaacgcc cgctgtgccg
     5461 ccggtgagga ttcccgactt gccgaagacc agcaccgcca ccgcgaacag cagcgtgccg
     5521 gtcgcaattg cctgcgcgtc ttcgaaggga gtgtggggcg acgtgcccgc ccgagcgcgg
     5581 ccggactcgg ccatcagttc aggtggcgcg cacgggcaac gggaaaccct tgccggcctg
     5641 gcgcagcttg cccaccacgt gcatttcgca gcgcttgcag tcgaagcgca gcgtgaattt
     5701 ctggccgccg ttttccagcg tcagcgggtc ggggcggata cccttcactt cctttgggca
     5761 gagattgaag ctgtagcgca ggcagtgtcg cgtgatcatc agcgatactt ctccgcgctc
     5821 ttggttggtc tcgtaagcat cggcgatctt gagtacgccg tggcggatgt agaaagcacg
     5881 tgcgctggca ttgagcacgt tggcgaggaa ggacagttct gtctcggggt agggcggcgc
     5941 cggaacccgc gcgtcggcgc gccgcggtcg tgcgtagcgt tgtcggcgcg ctgcctcgag
     6001 cgactcgatc gccgcgcggc gcagtccgtt gagtgctgcc gccgacagcc tcggcacggt
     6061 cgtcgacgcg acctctatcc ggcgtgccgt gaagatcgtg ctgccgagct ttccgagttg
     6121 ctggcggatc cgctccgctt cacgcaccgg ctcgatggcg gcctcgaact gacccggcag
     6181 gtccgcgctc gcggcgatgc cgtcgctgtc ggtcgcctgc agacggatgc cgtcatcggt
     6241 ggcggaaaac accaggtcga tctcaatttg ccgcgtcgcg ctgtcgtgtt cgagctggcg
     6301 gtcaaaggcg tggtcgcgat tgcggaacag cggcgtgccg gcgacgggaa gcagcacgcc
     6361 gcgcaggacg tgcaggcgct gccggtcggc gagttgctcg acgcgattga cgcgtagtcc
     6421 ggccagctcg ccagaggcgt agaagaaggc gatgccgtcg ccgttgccga gcggcggcgt
     6481 cggcgccacg tcgattgcct cgcgccccac cgcgaggact tcgccgatcg gctcgccggc
     6541 tgccttaggc gtgtcgaagg cgccgatgtc ggcctgccgc tcgtgcgtga agtagtcggt
     6601 tgcgccgcgg cggaaggtct tgcccggatc gggtgtgaag ccgaagcggc tgtggcccga
     6661 cgacgcggag caccagcgtg ggtcgtcggc gatcagccga tcgagcagca gccggtagtg
     6721 cgcggtgatg ttcttgacgt aggcgcgatc cttcagccgt ccctcgatct tgaacgagcg
     6781 gatgccggct tcggcaagcc cggcgaggtt ggcgctctgg tcgttgtcct tgagcgagag
     6841 caggtgcttg tcggccacga tcgttgtctc gccgtcgctg agcgagaacg gcaggcggca
     6901 gatctgcgcg cactcaccgc ggttggcgct gcgtcccgtg agcgcgtggc tgatactgca
     6961 ctggccgctg tagctgacgc agagcgcacc gtgaatgaag tattcgagcg gtaccgtcgt
     7021 cgtcgcggtg atcgccgcaa tctcggtcag cgacagttcg cgcgccagca ccacctgcga
     7081 gaagccgaca gcttcgagga aggcgacctt ggccggactg cggttgtcca tctgcgtgct
     7141 ggcgtgcagg gcgatcggtg gcagctcgag atcgagcagg cccatgtcct ggacgatcag
     7201 cgcgtcgacg ccggcattcc aggcatccca tgcgagttgc cgcgccggct cgagttcgtc
     7261 gtcgcgcagt atggtgttca gggtcagata gacgcgcgca tggtagcgat gcgcgtgctc
     7321 gactaggcgc gcgatgtcgc tcatcgtgtt gtcggcgccg ctgcgcgcac cgaagccggg
     7381 gccgccgata tacacggcat cggcgccgtg gtcgatcgcg gcgatgccga tctcggcatt
     7441 gcgggccggg gcaagcagtt cgagcgggat tggcgcaggt gacatggcgg gctgtgtggc
     7501 aggcagcaaa gggcaggggc ggcaattatc gctcagctgg ccggcccgct tgaggcagaa
     7561 tgcgacgggt ctggaatacg gggtgcggcg gcgggtgatg cggcgatttc gcggcgggag
     7621 gtgtctggtg gcggcaattg cgatctcgct tgcgccggcg ccgtcgatcg cgcaatcggt
     7681 gtcgccagcc gacgaggcag cgggcgcgcc gccggcttac cgtgcaccgc taccgccggc
     7741 gaagcgaggc cgtgagatcg aactcatccg cgaactcggg gcctattaca caagcatcgc
     7801 cgtgcagatc ccgctcgccg atgcgccgct tgccgaaggt ggcgacgtcg gcgagatgga
     7861 ggtctatcgc cggctcttcg aggcctcggc cagcccgagc gcgctgctgc tcgaagccag
     7921 cgtctacccg atgccgctgc tcggtaccca cctgaagaag cactcgcccg atttctatga
     7981 cacgcgctgg ctcggcgtcg acatcgtgcc cgcggtcacc gccggattcc aggagccgtg
     8041 ggcgctggca gccttcgtcg gtgccgacat gcgcttcacg cgtcccggcg aggccgagcg
     8101 cgacaccaat cgcggctaca tgggctatct cgtctcggtc ggcaatcgtc acatccgcga
     8161 caacgtgatg atcgacgacg actggttcga gttggagtgg aagatgaagg gcgagcggat
     8221 tttcgttgcc gatcgcctga cctggagttt tcgcgtcggc acgcgctggc acgccaacgc
     8281 cgatattcgc gacacggtct acctcggcat cagccgctcc gataccaact acgggtcgcc
     8341 gatgctgcgc tggctcaaca acaccgaggt caggctgacg accgagttct cgcagcgtga
     8401 cttcgatttc ctgcggcagg agatcatctt cggcaagaag attccgctgg ctcactggtt
     8461 cagcgcgctg cagttcgatg tcggcgtgat ccacgagcag cgcggcaagt acacaggaca
     8521 actcggtgcg cagtctgccg gcggaacgac cttcatcttc cgtcccggcc tcgagttctg
     8581 aggccagggt ggaggcgctt cagaagcggt aaccgatgcc gccagtgagt atgtcggcgt
     8641 cgctgccgtc ggccgagatc aggtcgtagt cgacacggac cgacaggcga ttgtcgatgc
     8701 ggtaggcgcc gccgatgccg aaaccgagat cgaggctgtt gtcggagaag ccgccgccga
     8761 agggggcgaa gtcgacctcg acccggctgt atgagaggaa ggcgcgggcc tgcagcgaga
     8821 agcgagtgtc gggcagcggc acgtcgaagg tcgcgccacc ggcaaaggtg gtgccggtga
     8881 tgtcggtgcc gcgcgaatca ccgtcccagc gcgaaacgaa gccggtcagg ccgagtccct
     8941 tgacgccggc gagatccttc atgaagtcgg cgccgaactt gatggcgacg ccgtcgcgca
     9001 cgccctgcag gtcggccggg ccgatctggc cataaccgta gacgccagac tgcggactcg
     9061 cctgcgccag cgcggtcgtc gagcttgcgg caagcaccgc ggcggcaata aatcttttca
     9121 tcggtctctc catgggttga tgagttcgtg ttggattgcc gctgcggacg caggttcagc
     9181 caaatgaccc gggtgattcg acccggttcg ggtcgcgatg cccttcgagt gaccgggcag
     9241 cagcctttgg cgaatggcgc tcagtgcgcc tcagggattc gcttcggccg catcgagcgg
     9301 cacggcgagc cgtgcgaagt cgactgggcg cagatactgc agtaccgcct ttcccgattc
     9361 gtcgacggcc acatcgatac cctggagagg gtagagatag tgcacgcgct tgccgcgaag
     9421 caaggttcgc tcggcggggc cgaagcgggt ctcgaccatt gccgcgtcga ggcggatcga
     9481 cggcacgaaa gtcagtgaat cgacgtgcat cgccagtgcc gcgtcgaagt cggcgggcga
     9541 gagcgaccag cgacgggcgc cacttggctg cgctgccgac tggatggctc gctggcgggc
     9601 ggcatcgagc aacgatggtg gtgccgcggc ggctagtacc acggctccgg tgacgaaggc
     9661 cgactcgaca cgcgccgcgt agccttcgag ggcgagttgt cgatccggct gttcgatcag
     9721 cgccaactgc agatcgccac cgaatcgctg gcgtgcctcg ctcagcgtcg tcgtgccgag
     9781 ccgaatgccg aggacttcgc tggtcgatcc atctggcgag acagtgacct cccacggcgg
     9841 cggtagcgtg ttcacgcgtg gcggcggcgc cgaacgcaag gcgatccagg cggcaactat
     9901 cagcagggcg ggaacgacaa tcaacagttt tcgtgacgtg atcatgggcg gaggctaaca
     9961 gcagtggcag gacgtcaggc aagaaaaaac cccaggctaa gcccggggtt ttttggggta
    10021 ctgcggaggc tgaagaatta cttcttcttg ccgccggtcg tgcccatcgc cttgacggtc
    10081 gcgctggtgg cagcggcaac gttcttctcg gcgacttcag caacttcctt ggcaaccttg
    10141 ttcatgttgt cgaaggcgct gttggcggca gcaaaggccg actgcatggc ggcaacggca
    10201 acttccgaac cggccggggc cttgctcgaa gcttgggcga ccaagtcggc aacgttcttg
    10261 cggaagtcgg cgaactgggc ttcgaccagc ttggccagcg cctgctgggt gtcggtggtg
    10321 atcgcgtaga cgttctgcga gtaagaaacg gccttctcga cagccggctg ggccagcgac
    10381 ttctgggtct cgaccaggtc ctgaacgtcc ttgacgccca tcagggtctt ggcgttcgcg
    10441 gcgccgtccg acagcgcggc gcgggcggtg ttgaggttga gggcggcaac gcgctcggcg
    10501 ctggcgaggg ccgtgttggc aacggtcagc agggcgtcaa cggcggcttt gttggcggca
    10561 gcaaactgct cggggatctg gttcgtcatg gcaatactcc taattggttt tggaaagaaa
    10621 actcgcggcc aaatcagcag tcaaagccaa tgttgcaccg cacaactgcc attctaccga
    10681 tcgccgaagc gatgtcaaga actttttgtg cggtgcaata gaaaaagatg gaaattcaat
    10741 aaaaacaata cattgcgact ttacggcggc gctttgggaa agcgccggaa gcgatacttc
    10801 ggatgctgcg gcgcggcttc cggcctttcg ggcggcagcg acatgcgtgg gttccgtcag
    10861 atatccgtcg gcgtgccgat gttctcgcca cgttcgaaga caacgttggc gcgaccgaca
    10921 atcagcgtgt cgagggcgcc gatgcgtgcc acatctttgt tggtataggg caggcggtga
    10981 aggatgtagc gcatagcgtt gagtcgtgcg cgcttcttgc agtcggactt gatgacggtc
    11041 caaggcgcat cggcggtgtc ggtgtagaag aacatcgctt ccttggcctt ggtgtagtcc
    11101 tcccatttgt cgagcgacgc caggtcgatt ggcgagagct tccactgctt gagcggatgg
    11161 accttgcgct ccttgaagcg gcggcgctgc tcctcgcggc tgaccgagaa ccagaacttg
    11221 acgaggtgga tgccgttgcg aaccatcatg cgctcgaact cgggaacctg gcgcatgaat
    11281 tcgtggtact cggcatccga gcagaaaccc atgacgcgct cgacgccggc gcggttgtac
    11341 caggagcggt cgaacatgac gatctcgccg gcggtcggca ggtgctcgac atagcgctgg
    11401 aaataccact ggccgcgctc gatctccgag ggcttttcca gcgcgacgac gcgcgcgccc
    11461 cgcgggttga gattctccat gaagcgcttg atcgtgccgc ccttgccggc cgcgtcgcgg
    11521 ccttcgaaaa gaacgactac cttctggccg ctttccttga cccaggcctg cagcttcagc
    11581 aactcgactt gcagacgata tttctgccgc tcgtaggtcc gccgcgccat caggttcttg
    11641 tagggatagc caccctcgcg ccaaccgcgc gcgagcacgt cgtcggggtg ctcgtcgcgt
    11701 tcgtcggctg cgatgcgccg ctgcttaagc aggttcttca gcagcgagat cgcttcttcg
    11761 ttgggtacgc cggaaatcac gtcgttcata gccgctatgc ggcggacgcg cgcgccatct
    11821 tcggcctcac gcgtttcgaa attctcgatg ccggacggcg tagtgctcgg tgcgacgtcg
    11881 ccggccgctg cgctgcgcgt gcggcgctgg gtcggcttgc cattgcgggc ggcgcggtcc
    11941 ttgcgcggga tggcggccgt agcaggcctc gctgccgcgg gacgtttgcc tttggtggcg
    12001 gccttgtcgg cgctgctggc cttcctggcc accgctttgc gggcccgtcg gagcgtgccg
    12061 gtagctgctg attcgccggc ctgtttcgtt gctttgtcgg ctgcttcggt ttttggcgtg
    12121 tcggtcttgg ccatgtcttc cttctccttt gcggaaccag ttcttatcgg ctgatcggct
    12181 tgtagcggat gcgtttgggc ttggcacctt cctcgccgag tcgtttcttc ttgtcagcct
    12241 cgtattcctg gtagttgccg gcgaagaagc tccactgcga ttcgccctcg gcggcgagga
    12301 tatgcgtgca gatgcggtcg aggaaccagc ggtcatggct gatgatcagc gccgagccgg
    12361 cgaactcgag tagcgcgtct tcgagcgcac gcagcgtctc gacgtcgaga tcgttcgagg
    12421 gctcgtcgag cagcagcagg ttgccaccgg tcatcagggt cttcgccaag tgcagccgac
    12481 cgcgttcgcc gcccgagagc ttgccgacga tcttctgctg gtcggcgccc ttgaagttaa
    12541 agcgtccgat gtaggcacgg ctcggcatct cgaacttgcc gatggtgatg aggtcgttgc
    12601 cgtcggcgag ttcgtcgaag acggtctttt ccgacgagag gttctcgcgg ctctggtcga
    12661 cgtaggccat cttgacggtc tggccgatca ccacttcgcc cgagtcgggt tgttcgctgc
    12721 cggtgatcat gcggaacagc gtcgacttgc cggcgccgtt ggggccgatg atgccgacga
    12781 tggcgcccgg cggaatcgag aacgacagct tgtcgatcag caggcggtcg ccaaaggcct
    12841 tcgtgacgtc cttgaattcg atgacctggc ccccgaggcg ctcggcgacc ggaatgaaga
    12901 tctcctgcgt ttcattgcgc ttctggtatt cggcactcga catttcgtcg aagcgggcga
    12961 ggcgggcctt cgacttggct tgccgcgcct tggggttact gcgcacccat tcgagttcct
    13021 gcttcatcgc cttcatccgc gcgccttcct gcttcgactc ggtttccagg cgcgcttcct
    13081 tctgctcgag ccagctcgag tagttgccct tccacgggat accttcgccg cggtcgagtt
    13141 cgagaatcca ttcggcggcg ttatcgagga agtagcggtc gtgggtgacg gccacgacgg
    13201 tgccgggaaa gcgcacgagg aactgctcga gccattcgac cgactcggca tcgaggtggt
    13261 tggtcggttc gtcgagcagc agcatgtcgg gcttcgagag caacaacttg cacagcgcga
    13321 cgcggcgctt ctcgccaccc gacagcgtct tgatcgatgc atcccaaggc ggcaggcgca
    13381 gcgcgtcggc ggcgatttcc atctggtgct cggtgtccga gccggtgctg gcgatgatcg
    13441 cctcaaggcg cgcctgttct tcggcgagct tgtcgaa
//
LOCUS       DATDAA010000148         2458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_500311,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000148 DATDAA010000000
VERSION     DATDAA010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500311"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VIS73_04825"
     CDS             complement(<1..421)
                     /locus_tag="VIS73_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEY5762508.1"
                     /translation="MSDDLKPASRTKDIGHEVGEHNVQVFGLDIHNPVFLVSAVLAVS
                     VVAGTLMFREQAAVLFADMRAWITVTFDWFFMISANLLVVYCLGVALSRLGRVRLGGP
                     DAVPRYGYPGWLAMLFAAGVGIGLMFFGVLEPVTHTIN"
     gene            complement(438..>2458)
                     /locus_tag="VIS73_04830"
     CDS             complement(438..>2458)
                     /locus_tag="VIS73_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY5762509.1"
                     /translation="KGRLPFRRDNFLQDGEASPDFKAIQARIVAKEFDEALRDLAAVL
                     ENDPDSTDALYMSAVCHRYRQEYESALDRIARLKALAPELGRAHQEEGHAYRDMGRAD
                     DALAAYTLACRFNPALEASWRGQVTILAKKGLNAKAAQARAQLDYLKALPAPLVAVMD
                     LIAEGKLLKAEDICRQFLKKVPHHVEAMRLLADIGLRLGVLDDAELLLEGATALDAEN
                     IRARIDYIKVLRKRQKFQKALEQAESLLERSPANPQFQSIYAIECMQCGDFETALATF
                     DRVLESVPGDPATLTSKGHALKTGGRYEEAVDAYRSVLASNPKYGEAFYSLANLKVYS
                     FNDEEIERMRAVESDAGLPHMDRVYVSFALGKAFEDRGDYETSFDYYARGNRLKRAQS
                     RYNAGQMSEDLRAQREACTAELFVARAGSGCDAPDPIFVVGLPRAGSTLIEQILSSHS
                     RVDGTLELPNIPSLSQRLRRRGRQAGNKPYPALLADLGAGELEEFGREYIRDTGIHRQ
                     GAPCFIDKMPNNFRHIGLIRLILPNAKIIDARRGAMACCFSGYKQLFAEGQEFSYDLA
                     DLGQYYRDYVELMDHWDRVLPGYVLEVRHEDVVRDLESEVRRMLEFCGLPFEEACLEF
                     HRTDRNVRTPSSEQVRQPIYTSGLEQWRNFEPWLGPLETALGPALAAS"
BASE COUNT          410 a          799 c          793 g          456 t
ORIGIN      
        1 tattgatcgt gtgcgtgacg ggttccagca cgccgaagaa catcaggccg atgccgacgc
       61 cggccgcaaa cagcatcgcc agccagccgg gatagccata gcggggaacg gcgtccggcc
      121 cgcccagcct gacgcgaccg agccgtgaca gcgcgacgcc gaggcaatag accaccagca
      181 ggtttgccga aatcatgaag aaccagtcga aggtgacggt tatccaggcg cgcatgtcgg
      241 cgaacagcac cgcggcctgc tccctgaaca tcagggtgcc ggcaaccacg gagacggcga
      301 gtacggcgga aaccaggaaa accgggttgt ggatatcgag gccgaatacc tgtacgttgt
      361 gctcgccgac ttcgtggccg atgtctttgg ttctcgaggc cggtttcagg tcgtcactca
      421 attatctgcc ccattcttca gcttgccgcc agcgcgggcc ccagcgccgt ctcgagcggt
      481 ccgagccagg gttcgaaatt gcgccactgc tcgaggccgc tcgtgtaaat cggctgcctg
      541 acctgctcgg agctcggcgt gcgcacgttg cggtcggtgc gatgaaattc gaggcaagct
      601 tcctcgaaag gcaggccgca gaattccagc attcgccgca cctcgctctc gaggtctctt
      661 acgacgtcct cgtgcctgac ctcgagcacg tagcccggca ggacccggtc ccagtggtcc
      721 atcagctcga cgtagtccct atagtactgg ccgaggtcgg cgaggtcata ggaaaattcc
      781 tgtccctcgg cgaacagctg cttgtagccg ctgaagcagc aggccatggc gccgcggcgc
      841 gcatcgatga tcttcgcgtt cggcagtatc agccttatga gcccgatgtg ccggaagttg
      901 ttgggcatct tgtcgatgaa acagggagcg ccctggcggt ggatcccggt atcccggatg
      961 tactcgcggc cgaattcctc gagttccccg gcgccgaggt cggccagcag ggcgggatac
     1021 ggcttgttgc cggcctgccg gccccgccgc ctgaggcgct gcgacaacga aggtatgttc
     1081 ggcagttcca gcgtgccgtc gacccggctg tgcgaggaca ggatctgctc gatcagcgtg
     1141 gagccggcgc gcggcagccc gacgacgaag atcgggtccg gcgcatcgca gccgctgccg
     1201 gcgcgagcga caaaaagttc ggcggtacag gcctcgcgtt gtgcgcgcag atcttcgctc
     1261 atctgccccg cgttatagcg gctttgcgcc cgcttgagcc gattcccccg cgcgtagtaa
     1321 tcgaacgagg tttcgtagtc gccgcggtcc tcgaaggcct tgcccagcgc gaaggaaacg
     1381 tacacgcggt ccatgtgcgg caggccggcg tcgctttcca cggcgcgcat ccgctcgatc
     1441 tcctcgtcgt taaacgaata gaccttcagg ttggcgagcg aataaaaggc ctcgccgtat
     1501 ttcgggttcg aggcgagcac ggaacggtag gcgtcgaccg cctcctcgta gcgcccgccg
     1561 gtcttcaacg cgtgcccctt ggacgtcagg gtcgcgggat cgccggggac cgactcgagc
     1621 acccggtcga acgtcgcgag ggccgtttcg aaatcgccgc actgcatgca ttcgatggcg
     1681 tagatcgact ggaactgggg attcgcgggc gatctttcga gcaggctttc ggcctgctcg
     1741 agcgccttct ggaatttctg gcgcttcctc aggaccttga tgtagtcgat ccgggcgcgg
     1801 atattctccg catccagcgc ggtcgcccct tccagcagca gctccgcatc gtcgagcacg
     1861 ccgaggcgca ggccgatgtc ggccagcagc cgcatggctt cgacgtgatg cggaaccttc
     1921 ttgaggaact ggcggcagat gtcctccgcc ttcagcagct tgccttccgc gatcaggtcc
     1981 atgacggcga ccagcggcgc cggcagcgcc ttcaggtagt cgagctgcgc ccttgcctgg
     2041 gccgccttcg cgttcagccc cttctttgcc aggatagtga cctggccccg ccagctggcc
     2101 tcgagcgccg ggttgaacct gcaggccagc gtatacgccg cgagcgcgtc gtccgcgcgg
     2161 cccatgtccc ggtaggcgtg cccctcttcc tggtgggcgc ggccgagttc cggcgccagc
     2221 gccttgaggc gcgctatccg gtcgagcgca gactcgtatt cctgccggta gcggtggcag
     2281 acggcgctca tgtacagggc atccgtactg tcggggtcat tctcaaggac cgcggccagg
     2341 tcgcgcaggg cctcgtcgaa ctctttggcg acgatcctgg cctggattgc cttgaaatca
     2401 gggctggctt cgccgtcctg caagaaattg tcccgtcgaa acggaaggcg cccctttc
//
LOCUS       DATDAA010000149         3289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_325552,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000149 DATDAA010000000
VERSION     DATDAA010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325552"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..436
                     /locus_tag="VIS73_04835"
     CDS             191..436
                     /locus_tag="VIS73_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762510.1"
                     /translation="MDYIWIVVVSLAVGLFGNRYVAGAGHSVTVDAAYAVAGGLFAAF
                     LFRTTVISNEVGLSGALVFAGIGAGLLLLARRSFGQI"
     gene            complement(642..1442)
                     /locus_tag="VIS73_04840"
     CDS             complement(642..1442)
                     /locus_tag="VIS73_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5762511.1"
                     /translation="MRRFLLLLSLLLVAGVARAQHSLSVTGPSGAQSFTAAELSASPQ
                     VTDVTITHDPVFNRAMNYRAVPTAMLLQKAGIGPDGYVQARAVDDFSVAIPARVLLSP
                     AAFLAVEDPAKPWPKLSKGGRREGIGPFYLVYRDGQRPSSEYWVYRLAALKLTDSPIK
                     RWPQLAVAADLATDAPARRGLERFVEVCFACHRFRGAGEGTQGPDLGTPMNPVAYFQP
                     AALKKLLRDPESVRSWPERKMPAFTPEMLSDSDIDAIIAWLTYKEGTR"
     gene            1819..2427
                     /locus_tag="VIS73_04845"
     CDS             1819..2427
                     /locus_tag="VIS73_04845"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY5762512.1"
                     /translation="MNDWIFWAVGGFLFAEVAGYFIHRLLHADKVRILSRNHMIHHLL
                     IYGPKRLRSEKYISPTAGDNSRFGIYGFGLEWIIPVVSMAAITLGVLHLIGVDWISRL
                     IFIGAGLLWGYFMFGVVHDAMHLKNTIFEKNRFLRPWFRYVRRLHDLHHLKINEQGLM
                     DKNFGICFFFMDRIFGTLSKSTTRLNKDNLKAALDRYSFIRG"
     gene            complement(2846..>3289)
                     /locus_tag="VIS73_04850"
     CDS             complement(2846..>3289)
                     /locus_tag="VIS73_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY5762513.1"
                     /translation="KWRFFERGALSLGFKPGITLPTGDEKKFLGAGRVTWGALLIGSY
                     EPGPLAIHSHVGYRYYDNVVDLKTSLWHVSVASTWQLVESLKLVADLSRDTNPVRSGR
                     TPLDYVILGAIWTPRKYLDLDFGYRHGASGPALDRGLLAGLTLHW"
BASE COUNT          604 a         1027 c         1019 g          639 t
ORIGIN      
        1 tcccggactg tcgccgctgt tcgacagctc gaaggcggcg actctgtacg cgctcgagct
       61 cggcagggcg ctggcccgcg agcggtcgtc caggctgagg gtctgaaaac cataccccgc
      121 cgtcggtgcg gccagaccgc ggcgggcacc tcatcgtccc gcgggctgga acaggatata
      181 gggtgatcga atggattaca tctggatcgt cgtcgtttcc ctcgccgtcg ggctgttcgg
      241 caatcgctac gtcgcgggcg ccggccacag cgtgaccgtc gacgcggcgt acgccgtagc
      301 gggcgggctg ttcgccgctt ttctttttcg cacgaccgtc atctcgaacg aagtcggctt
      361 gtccggagcg ctcgtgttcg ccggaatcgg cgccgggctg ctgctactcg cacgccgcag
      421 cttcggccag atctgagcgg ggcttcgatg catgagcgct tccgcgcgtg caccgcatgt
      481 acagcatggt cagccgcggt agcagccaga atgtggccat gctcgaaatg ctccagcgga
      541 gcgttacgcc aatcggggtg gcgcgtgtcc cgcccgaaat agcgccaaag cggtcgttga
      601 ttgaaggcga cacgcgaccc ggctgtaacg ttcgcttgcg gtcaacgcgt gccttccttg
      661 taggtgagcc aagcgatgat cgcgtcgata tcgctgtcgc tcagcatctc cggcgtgaag
      721 gccggcatct tgcgctcggg ccaggagcgc acgctctccg gatcgcgcag cagtttcttg
      781 agcgcggccg gctggaaata ggctaccgga ttcatcggcg tgccgagatc gggtccctgt
      841 gtgccctccc cggcgccgcg gaagcgatgg caggcgaagc acacctcgac gaagcgctcg
      901 aggcctcgcc gcgccggcgc atcggttgcc aggtcggcgg cgacggcaag ctgcggccag
      961 cgcttgatcg gactgtcggt cagcttgagc gcggcgaggc gatagaccca atactcgctg
     1021 ctcggtctct ggccgtcgcg atagacgaga tagaacgggc caatcccctc gcggcgaccg
     1081 cccttggaga gtttcggcca cggcttggcg ggatcctcga cggcaaggaa cgccgccggc
     1141 gagagcagca cgcgggccgg gatggcaacc gagaaatcgt cgactgcgcg cgcctgcacg
     1201 tagccgtccg gaccgatgcc cgctttctgt agcaacatgg ccgtcggcac ggcgcggtag
     1261 ttcatggcgc ggttgaaaac aggatcgtgc gtgatggtca cgtcggtcac ctgcgggctg
     1321 gcgctgagtt cggcggccgt gaagctctgc gcgccgctcg gtccggtcac gctgaggctg
     1381 tgctgcgccc gggcgacacc ggcgaccagc aatagcgaca gaagcaggag aaaccgacgc
     1441 atgcctccta tgctacaccg tcattctcac agaagaccct gcgcgcaacg acagcctgca
     1501 gggcgggtta tggccggtag gcgcccgcgc gggacggcct aaaccgaccc ggagcggccg
     1561 gtcgccccag cccaaacagc cccgccgccg ccgaacgtcc gcttcgggga aatcagatgc
     1621 gaccgggtca tcgataaaga tgaaaaatcg gcgaaaacct gcgaacaagt cttcactcct
     1681 gcacaacatc atttgtcgtt caatcaccgg ctcagcaagg acaggttcga aaagccttcg
     1741 aagaaatcat ccctcaggcc aaatccttac gttggcctaa cttgacctgc tcaccggcgc
     1801 tctctacact ggtctgtcat gaatgactgg attttttggg cagttggtgg ttttcttttt
     1861 gctgaagtgg ccgggtactt tattcatcgg cttttgcacg cggacaaggt gcggatcctg
     1921 agccgcaacc acatgatcca ccaccttctg atctacggcc caaaacgcct tcgctccgaa
     1981 aagtacatct cccccacagc aggggacaac tcgcgctttg gaatttatgg tttcggcctc
     2041 gagtggatca ttcctgtcgt ttcgatggcg gcgatcaccc ttggggttct tcatctgatc
     2101 ggcgtcgact ggatctcgcg cctgatcttc attggtgccg gtctgctctg gggttacttc
     2161 atgtttggtg tcgttcacga tgccatgcat ttgaaaaaca cgatcttcga aaagaaccgt
     2221 tttctccgtc cgtggtttcg atacgtccgc aggcttcatg accttcacca tctcaaaatc
     2281 aacgaacagg gactgatgga caagaacttc ggcatttgct ttttctttat ggaccggatc
     2341 ttcggaaccc tcagcaaatc gaccacccgg ctcaacaaag acaatctgaa ggctgccctc
     2401 gatcgctact catttatcag gggctaagct cgaaaacgcc cctgcaatcc ctgcaggctt
     2461 gcagccgatt ggggaagcct cagcaaagga taggaaatcg ctgtgccgcg aaacgcgcgc
     2521 ggtggcggtc tgttcggcct cggtgagcgg tgcttgtggc tgggattgat cgtggccgcg
     2581 gctgcgagcc cggcgcgctc gctgcctgag ggcaaataag ccgggaaagt tcccggccat
     2641 ttccgactct ttttgccctc cctacccggg gccgccgccc ggtgcgaatc tggcagaaaa
     2701 caataacgct tttcccggac caagtcactg acaggaaatg gcttttttga ctccctgtgg
     2761 ttcacggaga tcgatcggaa attgcttttt tgacgctccg cggtccaggg aaaggacagg
     2821 agatcccttc gcccctgcgc cacccctacc agtgcaacgt caaccccgcg agcagcccgc
     2881 gatcgagcgc cggcccgctc gcgccgtgcc ggtagccaaa gtcgaggtcg aggtacttcc
     2941 gcggcgtcca gatcgcgccg aggatgacgt agtcgagtgg cgttctgccg ctgcgcacgg
     3001 ggttcgtgtc gcggctgaga tcggccacca gcttcagcga ttcaacgagc tgccaggtcg
     3061 aagcgaccga gacgtgccat agcgaggtct tcaggtcgac gacgttgtcg taatagcggt
     3121 agccgacgtg cgagtggatc gcgagcggcc cgggctcgta ggagccgatc agcagcgcgc
     3181 cccaggtgac gcgcccggcg ccgagaaatt ttttctcgtc gccggtcggc agcgtgatgc
     3241 ccggcttgaa gccgaggctc agcgcgccgc gctcgaaaaa gcgccactt
//
LOCUS       DATDAA010000150         8795 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_185707,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000150 DATDAA010000000
VERSION     DATDAA010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8795
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185707"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..351)
                     /locus_tag="VIS73_04855"
     CDS             complement(40..351)
                     /locus_tag="VIS73_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HEY5762514.1"
                     /translation="MATDSSYEKTYRDRALKLYPHICGRCGREFSGTRLKELTVHHKD
                     HNHSNNPPDGSNWELLCIYCHENEHARQLDSAGRSDSGQTVAPATHNPFAGLGGLLKK
                     P"
     gene            complement(371..691)
                     /locus_tag="VIS73_04860"
     CDS             complement(371..691)
                     /locus_tag="VIS73_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEY5762515.1"
                     /translation="MPFADNLKKLPGVSHLAAINLLDAEGAVVATIENKSGSQGSLSV
                     YNHLAQHFGAITPEAAKKGLELFAEHTDDAKQHPGKHPNIDRLLTLAAEKRTLRIKHV
                     FAAV"
     gene            complement(712..2079)
                     /gene="purB"
                     /locus_tag="VIS73_04865"
     CDS             complement(712..2079)
                     /gene="purB"
                     /locus_tag="VIS73_04865"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY5762516.1"
                     /translation="MALSPLNALSPIDGRYGTKVDVLRPHFSEYGLIHRRLQVEIEWL
                     KALAAEPALGEIAPFSAATVAQLDALVSDFSEADAAEVKAIEATTNHDVKALEYWIKG
                     KVGDNPEVARVAEFIHFACTSEDINNLSHALMLQTARADVLLPTLDRVIARIRELAHR
                     YAEVPMMCRTHGQPATPSTLGKEMANVVYRLQRGRERIAAVSLLGKINGAVGNYNAHS
                     VAYPALDWEAFGKRFVESLGLEFNPYTIQIEPHDAMAELYDAFARVNTILIDFDRDLW
                     GYISLGFFKQKLKAGEIGSSTMPHKVNPIDFENSEGNLGLANAVLKHLSEKLPISRWQ
                     RDLTDSTVLRNMGVGLGYALLGYDSCLRGLGKLEADPAKMRADLDANWELLAEPIQTV
                     MRRYAVPEAYEKLKELTRGQRVTREGMQAFVQTLEIPQAAKQELLALTPWDYIGKAAE
                     LAKRI"
     gene            2252..2860
                     /locus_tag="VIS73_04870"
     CDS             2252..2860
                     /locus_tag="VIS73_04870"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY5762517.1"
                     /translation="MKLIGSLTSPYVRKVRFVLAEKKIEVDFVIESPHTADSTVPDFN
                     PLGKIPVLVLDDESTLFDSRVIVEYLDNMTPNNKLLPAPSRERIAVKRWEAACDGCLD
                     AAVLVVMENKRPAKERSKTWVARQMEKVGRSVDFAAAELGEDSWCMGTHFSLADITLG
                     CALGYLDFRFPELDWRGRHANLAKLYEKLMQRPGFVETVPHD"
     gene            complement(2936..4063)
                     /gene="mnmA"
                     /locus_tag="VIS73_04875"
     CDS             complement(2936..4063)
                     /gene="mnmA"
                     /locus_tag="VIS73_04875"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY5762518.1"
                     /translation="MEETVVVGLSGGVDSAVAALLLKQQGYRVLGLFMKNWEDDDDSE
                     YCSSRQDLIDAMAVADRIGIDIEVVNFAGEYRERVFGEFLREYQAGRTPNPDVLCNSE
                     IKFRAFLDHALRLGATKIATGHYAGVRRVVGDHGEEFQLLKAEDGTKDQSYFLHRLSQ
                     EQLSKAIFPLATHYKREVREIAEKAGLHVSEKKDSTGICFIGERPFKEFLMRYLPAQP
                     GEIRELDTERVLGGHDGITFHTIGQRKGLQIGGVKEKGQRGGGDHEAWFVAGKDVANN
                     RLYVVRGHDHPALQADSLAAGDCHWISGRLPHTNWVYAAKTRYRMEDAPCEIDRCEGE
                     TAEIHFALPQWAVTPGQSVVVYESNVCLGGGIITGGCKLAA"
     gene            complement(4067..4522)
                     /locus_tag="VIS73_04880"
     CDS             complement(4067..4522)
                     /locus_tag="VIS73_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY5762519.1"
                     /translation="MIWKPNVTVAAVIRRDGRYLLVEEDTDDGRRFNQPAGHLDPDES
                     LLDAVIREVREETRYDFRPTALVGIYLWPHPMRATTYLRFAFRGELVGHDAGRSLDTG
                     IIEAHWLSREELAALSGQHRSPLVMQCIADFEAGCTAPLELLHHYQKAA"
     gene            4669..5589
                     /locus_tag="VIS73_04885"
     CDS             4669..5589
                     /locus_tag="VIS73_04885"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEY5762520.1"
                     /translation="MSMADRDGFIWYDGKLVPWREATTHVLTHSLHYGMGVFEGVRAY
                     KTERGTAIFRLAEHTDRLFKSAHIFQMKLPYDRETLNEAQKEVIRANKLESGYLRPLA
                     FYGSEKMGVSPKGASVHVVVAAWPWGAYLGAEGIEKGIRVKTSSFTRHHVNISMVRAK
                     ACGHYINSILANNEATNDGYDEALLLDPEGYVSEGAGENIFIVRAGRIYTPDLTSCLE
                     GITRATVMQLAGELGIEVVEKRITRDEVYCADEAFFTGTAAEVTPIRELDGRVIGAGR
                     RGPVTERLQSLYFDAVYGRSAKHANWLALV"
     gene            5598..5792
                     /locus_tag="VIS73_04890"
     CDS             5598..5792
                     /locus_tag="VIS73_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEY5762521.1"
                     /translation="MTTNSKEKIEITAKQLPLACPTPNSPLWSRHPRVFLDIATTGEA
                     VCPYCSAHYVLTGELPKGHH"
     gene            5823..6254
                     /locus_tag="VIS73_04895"
     CDS             5823..6254
                     /locus_tag="VIS73_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5762522.1"
                     /translation="MPRTLFPTPEDAEQAFYEAAEAGDLDALMAVWAEDDEVVCVHPN
                     GERLVGQGPIRESWRKILGSGRKLQVRLEQPVRWQGLMLATHSAIETVLIDGESEALH
                     LAATNVFVRSATGWRLLVHHASPVHGPSAQTNSGGTPPILH"
     gene            6275..7651
                     /locus_tag="VIS73_04900"
     CDS             6275..7651
                     /locus_tag="VIS73_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEY5762523.1"
                     /translation="MPFLPAEIFKAYDIRGIVGKSLTREVAFQIGRALATIAHERGGA
                     PIAVGRDGRLSGPELAGALMEGIRAGGIDSIDIGMAPTPVTYFAGHQLGNQSVVSVTG
                     SHNPPDYNGFKMVIAGETLYGEAIQDLRQRIESGALSSGAGQARKASVDDAYLERIIG
                     DVKLARPMKVVVDCGNGVAGELAPKLLRGLGCQTVDLFCEIDGNFPNHHPDPSKPENL
                     EDVVHALRETDAEIGLAFDGDGDRLGVVTKDGEVIFADRQLMLFAADVLARVPGGEII
                     YDVKCTRQLARWIGEHGGKATMWKTGHALIKAKLRETSAPLAGEMSGHMFFKERWYGF
                     DDGLYAAARLLEILSRSADASAVLKALPKSNSTPELNIAMNEGEPFALLDRLREKARF
                     DGSVETITIDGLRVEFADGFGLARPSNTTPVVVLRFEADSDAALERIQAQFRHAIDSV
                     WPGLKLPF"
     gene            7668..>8795
                     /locus_tag="VIS73_04905"
     CDS             7668..>8795
                     /locus_tag="VIS73_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5762524.1"
                     /translation="MSQPIYLGRQPILDAKLHLAGYELLFRSGTTNAAQFADGTQATA
                     SVVAHAFTELGIGDALGFRRGFINVDSVFLLDDAIELLPPDQVVLEILENVSATPQII
                     ARVEALRAAGFLFALDDLISIADPRLPLLPLVHIVKLDLPQLGETAVTELVQALRARC
                     SAKLLAEKVDNAEQLKFCKSLGFDYYQGYFFAKPSILQGRRIDNSRLSLLKILAQLLE
                     DADTALLEKGFKESPGLTVNLLRLVNSVATGLRVKITSLRHAITILGRRQLQRWLQLL
                     LYTDPSGKDSLAENPLLQLAATRGRLMELLAVEVAPRDERLADYAFMCGVMSLTPALF
                     ERPLEEVLAQVNLAAPICDALIERKGRLGELLALVESTETED"
BASE COUNT         1577 a         2896 c         2781 g         1541 t
ORIGIN      
        1 ctggcaaaac ggcgggcgtc ggcgcccctg agctggtcac tacggtttct tgagcaggcc
       61 gccgaggccg gcaaagggat tgtgcgttgc cggcgcgacg gtctggcccg aatcacttcg
      121 cccggccgag tccagctgcc gagcgtgctc gttctcgtgg cagtagatgc acagcagctc
      181 ccaattgctg ccgtcgggcg ggttgttgct gtgattgtgg tccttgtggt ggaccgtcag
      241 ctccttcagg cgcgtgcccg agaactcgcg gccgcagcgc ccgcagatgt gcggatagag
      301 cttgagcgcc cggtcgcgat aggtcttttc gtagctgctg tcggtggcca tgatggtgtt
      361 cccgaggaga tcagacggcg gcgaagacgt gcttgatgcg cagcgtccgc ttctcggcgg
      421 ccagcgtcag cagtcggtcg atgttggggt gcttgccggg gtgctgcttg gcgtcgtcgg
      481 tgtgttcggc aaacaactcg agccccttct tcgcggcctc aggcgtgatc gccccgaaat
      541 gctgcgccag gtggttgtag accgagagcg agccctggct gcctgacttg ttttcgatgg
      601 tggcgacgac cgcgccctcg gcatcgagca ggttgatcgc cgcaaggtgc gagacgcccg
      661 gcagtttctt caggttgtcg gcgaatggca tggtggtttc ctccgttgcg ctcagatacg
      721 tttggcgagc tcggcggcct tgccgatgta atcccaaggt gtcagcgcca gcaattcctg
      781 cttggcggcc tgggggattt ccagcgtctg cacgaaggcc tgcatgccct cgcgggtcac
      841 ccgttggccg cgggtcagct ccttgagctt ctcgtaggct tctggcaccg cgtagcggcg
      901 catgacggtc tggatcggct cggcgagcag ttcccagttg gcgtcgaggt cggcgcgcat
      961 cttcgccgga tcggcctcga gcttgcccag gccgcgcagg caggagtcgt agccgagcag
     1021 cgcgtaaccg aggccaacac ccatgttgcg cagcaccgtg gaatcggtga ggtcgcgctg
     1081 ccagcgtgag atcggcagct tctcggacaa gtgcttgagc acggcgttgg cgagtccgag
     1141 gttgccttcg gaattctcga agtcgatcgg attgaccttg tgcggcatcg tcgacgagcc
     1201 gatctcgccg gccttgagct tctgcttgaa gaacccgagc gagatatagc cccagaggtc
     1261 gcggtcgaaa tcgatgagga tggtgttcac gcgggcaaag gcgtcgtaga gctcggccat
     1321 cgcgtcgtgc ggttcgatct ggatcgtgta ggggttgaac tccagcccca gcgactcgac
     1381 gaaacgcttg ccgaatgcct cccagtcaag cgctgggtag gcgaccgagt gcgcgttgta
     1441 gttgccgacg gcgccgttga ttttgcccag cagcgaaacc gcggcgatgc gttcgcggcc
     1501 gcgctggagg cgatagacga cgttggccat ctccttgccc agcgtcgagg gcgtcgccgg
     1561 ctggccgtgg gtgcggcaca tcatcggcac ctcggcgtac cggtgcgcaa gttcgcggat
     1621 gcgggcgatc accctgtcca gcgtcggtaa cagcacgtcg gcgcgtgccg tctgcagcat
     1681 cagcgcgtgc gagaggttgt tgatgtcttc cgaggtgcag gcgaagtgga tgaactcggc
     1741 gacgcgcgcg acctcggggt tatcgccgac cttgccctta atccagtact cgagcgcctt
     1801 gacgtcatga ttggtggtgg cttcgatggc cttcacctcg gcggcatcgg cctcggaaaa
     1861 atcgctgacc agtgcatcga gctgcgcgac ggtggcggcc gagaacggcg cgatctcgcc
     1921 gagtgcgggc tcggcggcaa gcgccttgag ccactcgatc tcgacctgca gtcgacggtg
     1981 aatcagcccg tactcggaaa agtgcggacg cagcacatcg accttggtgc cgtaacggcc
     2041 gtcgatcggt gaaagggcat tgaggggcga gagggccatg gcggcgaatc ctgcgtgaaa
     2101 cggggcccgc attttatcgg ccacagccgc agaccggcaa acgcttgatg cgacggcagt
     2161 ttcgggcacg ggccgccgaa cgtgaattcc gtcgcgcgga cgcggaaagc ccggtgctag
     2221 aatcatcgcg ccccaacaca ccaaaaacat gatgaaactg atcgggtcgc tgaccagccc
     2281 ctacgtgcgc aaagtgcgct ttgtgctcgc cgagaaaaag atcgaggtcg atttcgtcat
     2341 cgaatcgccg cacacggccg attccaccgt tcccgatttc aatccgctgg gcaagattcc
     2401 ggtactcgtg ctcgacgacg agagcacgct cttcgactca cgcgtaatcg tcgagtacct
     2461 cgacaacatg acccccaaca acaagctgct gccggcgcca agccgcgagc ggatcgccgt
     2521 caaacgatgg gaggctgcct gcgacggctg ccttgatgcc gcggtgctgg tggtcatgga
     2581 gaacaagcgc ccggccaagg aacgaagcaa gacctgggta gcgcgacaga tggagaaggt
     2641 cggccgcagt gtcgatttcg ccgccgccga actcggtgag gacagctggt gcatgggcac
     2701 gcacttctcg ctcgccgaca tcacgctcgg ctgtgcgctg ggctatctcg atttccgctt
     2761 ccctgaactc gactggcgcg gccgccacgc caatctcgcc aagctctacg agaagctgat
     2821 gcagcgtccg ggcttcgtcg agaccgtccc gcacgactaa gtcaacaagg ctcgccacgc
     2881 gtcttggcaa tgcaaagggc caccgcggtg gcggtttgca ttggtcgagc cgcgctcagg
     2941 cagccagctt gcacccgccg gtgatgatcc cgccgcccag gcagacgttc gactcataaa
     3001 cgaccaccga ctgccccggc gtcaccgccc actgcggcag cgcgaagtga atctcggcgg
     3061 tctcgccctc gcagcggtcg atctcgcagg gcgcatcttc catgcggtag cgcgtttttg
     3121 ccgcgtagac ccagttagtg tgcggcagcc ggcccgatat ccagtggcaa tcaccggcgg
     3181 cgagcgaatc ggcctgcagg gccgggtgat cgtgcccgcg caccacgtag agccgattgt
     3241 tggcgacatc cttgccggcg acgaaccagg cttcgtgatc gccgccgccg cgctggccct
     3301 tctccttcac cccgccgatc tgcaggccct tgcgctggcc gatggtgtgg aaggtgatgc
     3361 cgtcgtgccc gccgagcacg cgctcggtgt ccagttcgcg aatctcgccc ggctgcgccg
     3421 gcaggtagcg catcaggaat tccttgaacg gcctctctcc gatgaagcag ataccggtcg
     3481 aatccttttt ctcggagacg tgcaggccgg ccttctcggc gatctcgcgc acctcgcgct
     3541 tgtagtgcgt ggccagcggg aaaatggcct tcgacaattg ttcctggctg agccgatgca
     3601 ggaaatagct ctggtccttg gtgccgtcct cggccttaag cagctggaat tcctcgccgt
     3661 gatcgccgac gacgcggcgc acgccggcgt aatgcccggt ggcgatcttt gtcgcgccga
     3721 gccgcagcgc gtggtcgagg aaggcgcgga acttgatctc cgaattgcac agcacgtccg
     3781 ggttgggcgt gcggccggcc tggtattcac gcagaaactc gccaaacacg cgttctcgat
     3841 actcgccggc gaagttgacg acctcgatgt cgatgccgat gcgatcggcc accgccatcg
     3901 cgtcgatcag gtcctgccgg ctagagcagt attcggagtc gtcgtcatcc tcccagttct
     3961 tcatgaaaag gccgagcaca cggtaaccct gctgcttgag caagagcgcc gcgactgcgg
     4021 aatcgacgcc gcccgaaagg ccaacgacta cggtttcttc catggttcag gctgccttct
     4081 gatagtgatg caggagttcc agcggcgccg tgcaccccgc ctcgaaatcg gcgatgcact
     4141 gcatgaccag cgggctgcga tgctggcccg agagggccgc aagttcctcg cggctcagcc
     4201 agtgcgcttc gatgatgccg gtatcgagcg agcgccccgc atcgtgcccg accaactcac
     4261 cgcgaaaggc gaagcgcaga taggtagtcg cacgcatcgg gtgcggccac aggtagatgc
     4321 cgactagcgc cgtcggccga aagtcgtagc gggtctcctc gcggacctcg cggatgacgg
     4381 cgtcaagcag cgattcgtcg gggtcgaggt ggccggccgg ctgattgaaa cgacggccgt
     4441 catccgtgtc ttcctcgacc agcagatagc gcccatcgcg gcgaatcacc gcggccacgg
     4501 tgacgttcgg cttccagatc atggctcact ccagtggccc ggattctacc tgcgggcgcg
     4561 ctgcgagcgc gacgaatccg ctagaatatg gcgcttcggc gcatgctcag cggcgccgcc
     4621 cagatcaagc cggcagcccc gggctgctgc aaacaggaga gtggagacat gtcgatggcc
     4681 gatcgcgatg gattcatctg gtacgacgga aagctcgtgc cctggcgcga ggcgacaacg
     4741 catgtactca cccattcgct gcactacggc atgggcgtct tcgagggcgt gcgcgcctac
     4801 aagaccgagc gcggcacggc gatcttccgt ctggccgaac acaccgaccg gctgttcaag
     4861 tcggcgcaca tcttccagat gaagctaccc tacgaccgcg agacgcttaa cgaagcgcag
     4921 aaggaagtga tccgcgccaa caagctcgaa tcgggatacc tgcgtccgtt ggccttctac
     4981 ggctccgaga aaatgggcgt ctcgcccaag ggcgccagcg tgcacgtggt cgtcgccgcc
     5041 tggccctggg gtgcctacct cggtgccgag ggcatcgaga agggcatccg cgtcaagacc
     5101 tcgtcgttca cccggcacca cgtgaacatc tcgatggtgc gtgcaaaggc ttgcggccac
     5161 tacatcaatt cgatcctagc caacaacgaa gcgaccaacg acggctacga cgaagcactg
     5221 ctgctcgatc ccgagggcta cgtctcggaa ggcgccggcg agaacatctt catcgtccgc
     5281 gccggcagga tttacacgcc ggacctgact tcctgcctag aaggcatcac ccgtgccacg
     5341 gtcatgcagc tcgccggcga actgggcatt gaggtggtcg agaagcgcat cacccgtgac
     5401 gaggtctact gcgccgacga agcatttttc accggtaccg ccgccgaggt gacgccgatc
     5461 cgcgaactcg acggccgcgt catcggtgcc ggccgtcgcg gcccggtcac cgaacgtctg
     5521 caaagccttt atttcgatgc ggtctacggc cgcagcgcca aacacgccaa ctggctcgcc
     5581 ctagtctgag acctgccatg accaccaaca gcaaagaaaa aatcgaaatc accgccaaac
     5641 aactgccgct tgcctgcccg acgccgaatt cgcctctctg gtcgcggcat ccgcgcgtat
     5701 tcctcgacat cgccacgacc ggcgaagcgg tttgcccgta ttgcagcgcg cactacgtgc
     5761 tgaccggcga gctgcccaaa ggccaccact aatcccggca cgcggcgcgg aatcgccccc
     5821 cgatgccgcg cacgctgttt ccgacgcccg aggatgccga gcaggcgttc tacgaggcag
     5881 ccgaggctgg cgacctcgac gcgctgatgg ccgtctgggc cgaggacgac gaggtcgttt
     5941 gcgtacatcc caacggcgag cgactggtcg gccaggggcc gatccgcgaa agctggcgca
     6001 agattctcgg cagcggccgc aagctgcagg ttcgacttga gcagccggtc cgctggcagg
     6061 gcctgatgct cgccacccac agcgccatcg agaccgtgct gatcgacggc gaaagcgagg
     6121 cgctgcatct cgccgcgacc aatgtcttcg ttcgtagcgc caccggctgg cgcctgctgg
     6181 tccaccacgc ctcgccggtg cacggcccga gcgcacaaac caattcgggc ggcacgccgc
     6241 ccatcctcca ctgactcccc gcaggagaaa cccaatgcca tttctgcccg ccgaaatctt
     6301 caaggcctac gacatccgcg gcatcgtcgg caaatcgcta acgcgcgagg tcgccttcca
     6361 gatcggccga gcgctggcaa ccatcgccca tgagcgcggc ggcgccccga ttgcggtcgg
     6421 ccgcgatggc cgcctctcgg gcccggaact ggccggggca ctgatggaag gcatccgtgc
     6481 cggcggcatc gacagcatcg acatcggcat ggcaccgacc ccggtgacct acttcgccgg
     6541 ccaccagctg ggcaaccaga gcgtggtctc ggttaccggc agccacaatc cgccggacta
     6601 caacggcttc aagatggtca tcgccggcga aacgctctac ggcgaggcga tccaggacct
     6661 gcggcagcgc atcgaaagcg gggccttgtc gagcggcgcc gggcaggcgc gaaaagcctc
     6721 ggtcgacgac gcctaccttg aacgcatcat cggcgacgtg aagctcgcgc ggccgatgaa
     6781 ggtggtcgtc gactgcggca acggtgtcgc aggcgagctg gcgccgaaac tgctgcgcgg
     6841 actcggctgc cagaccgtcg accttttctg cgagatcgat ggcaactttc ccaaccacca
     6901 tcccgatccg tcgaaaccgg aaaatctcga agacgtggtg catgcgctgc gcgagaccga
     6961 tgccgaaatc ggcctggcct tcgacggcga cggcgaccgg ctcggcgtcg tcaccaagga
     7021 cggcgaggtg atcttcgccg atcggcaact gatgctgttt gccgccgatg tgctcgcgcg
     7081 cgtccccggt ggcgagatca tctacgacgt caaatgcacg cggcagctcg cccgctggat
     7141 tggcgaacac ggcggcaagg cgacaatgtg gaagaccggc catgcgctga tcaaggccaa
     7201 attacgcgaa accagcgcgc cgctggccgg cgagatgagc ggccacatgt tcttcaagga
     7261 gcgctggtac ggttttgacg acggcctcta cgccgcggcg cgcctgcttg aaatcctgtc
     7321 gcgctcggcc gacgccagtg ccgtactgaa ggcgctgccg aagagcaaca gcactcccga
     7381 actcaacatc gcaatgaacg agggcgagcc cttcgcgctg ctcgaccgcc tgcgcgagaa
     7441 agcgcgcttc gacggctccg tcgaaaccat caccatcgac ggcttgcgcg tggaattcgc
     7501 cgacggcttc ggcctcgccc ggccctcgaa cacgacgccg gtggttgtgc tgcgtttcga
     7561 ggccgatagc gacgcggcac tggagcgcat ccaggcacag tttcgccacg ccatcgacag
     7621 tgtctggccc ggcctaaagc tgcccttcta gcaccgcacc ccctgcgatg agccaaccca
     7681 tctatctcgg ccgccagcca atcctcgacg ccaagctgca tctcgccggc tacgaattgc
     7741 tgtttcgcag cggcacgacc aacgccgcgc agttcgccga cggcacgcag gcgaccgcgt
     7801 cggtggtcgc ccatgccttc accgaactcg gcatcggcga cgcgctcggg tttcgccgtg
     7861 gcttcatcaa cgtcgattcg gtcttcctgc tcgacgatgc gatcgagctg ctgccgcccg
     7921 atcaggtcgt gctcgaaata ctcgagaatg tctcggcaac gccgcagatc atcgcgcgcg
     7981 tcgaggcgct gcgtgccgcg gggtttctct tcgctctcga cgaccttatc tcgattgccg
     8041 acccgcgcct gcccctcctg ccgctggtgc acatcgtcaa gctcgacctg ccgcaactgg
     8101 gcgaaaccgc ggtcaccgaa ctcgtgcagg cgctgcgcgc acgctgcagc gccaagctgc
     8161 tcgcggagaa ggttgacaac gccgaacaac tcaaattctg caaatcgtta ggcttcgatt
     8221 attaccaggg ctacttcttc gccaagccgt cgattctgca gggccgccgc atcgacaact
     8281 cgcgcctgtc gctactgaag atactcgccc agttgctcga ggacgccgac acggcgctgc
     8341 tggaaaaggg cttcaaggaa tcaccgggtc tcacggtcaa cctgctgcgc ctggttaact
     8401 cggtggcgac cggcctgcgt gtaaagatca cttcgctacg ccacgccatc accattctcg
     8461 gccgccgcca actgcaacgc tggttgcaac tgctgctcta caccgacccc tcgggcaagg
     8521 acagcctcgc cgagaacccg ctgctacaac tcgcggcaac ccgcggccgg ctgatggagc
     8581 tgctcgccgt cgaagtggcg ccgcgcgacg agcggctcgc cgactacgcg ttcatgtgtg
     8641 gcgtcatgtc gctgaccccg gcgctgttcg agcgtccgct cgaagaagta ctggcgcagg
     8701 tgaacctcgc cgccccgatt tgcgatgcgc tgatcgagcg caaggggcgc cttggcgaac
     8761 tgctggcgct cgtcgaatcg accgaaaccg aagac
//
LOCUS       DATDAA010000151         9370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_500475,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000151 DATDAA010000000
VERSION     DATDAA010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9370
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500475"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="VIS73_04910"
     CDS             <1..1436
                     /locus_tag="VIS73_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY5762525.1"
                     /translation="ISLFDDEVERLDLFDPLTGQIERRLSRFTVYPSSHYVTPRSTVL
                     RAIEAIKQELRERIAFFDGQTKLIEAQRIAQRTRFDLEMLDELGFCKGIENYSRHLSG
                     RSAGEPPPTLIDYLPDDALIFIDESHVSIPQVGGMFRGDLSRKQNLVDYGFRLPSALD
                     NRPLRFDEFERRLRQAIFVSATPSDYEAQHAGAVVEQLVRPTGLIDPDLLVRPAANQV
                     DDLLHEIRRRTAVGERVLVTTLTKRMAEDLTDYLADNGVRVRYLHSDIDTVERVEIIR
                     DLRLGEFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSARSLIQTIGRAARHLNG
                     TAILYADRVTDSMRRAIDETDRRRRKQVEFNTLNSITPKSVSKRVKDIIDGIYDPQAE
                     RTAMALAEERVAYATMDQASLAREIRELERQMSEHARNLEFEQAAAARDRLFRLRESA
                     LGLATSGAGGMAKGAAPQKQPRRRRGR"
     gene            1468..2013
                     /locus_tag="VIS73_04915"
     CDS             1468..2013
                     /locus_tag="VIS73_04915"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEY5762526.1"
                     /translation="MVRNAPTIRKQGLPRAATQTTREVRVLFVCMGNICRSPTAEAVL
                     RKALKEHGIDDRVEVDSAGTHGYHVGEPPDPRTQRAAESRGYDLSSIRARKVAPQDLE
                     YFDLVLAMDRSNLENLQRICLPDFGDRLGLFMPYSRAFDDDEVPDPYYGLGHGFDLVL
                     DMIEDASAGLIVEIKKRLAAK"
     gene            complement(2046..4784)
                     /locus_tag="VIS73_04920"
     CDS             complement(2046..4784)
                     /locus_tag="VIS73_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY5762527.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQKLIDLDIESAAKEQRKSNIYKG
                     VITRIEPSLEAAFVDYGADRHGFLPFKEVSRSYFKPGVDASRAKINEALKEGQELVVQ
                     VDKDERGNKGAALTTYVSLAGRYLVLMPNNPRGGGVSRRVEGDERSELREILAQIEVP
                     DGMSVIARTAAIGRALEELQWDLNYLQQLWSAIDGVAGELKAPLLIYQEGSLVIRAIR
                     DYFQPEIGEILIDTEDIYEQAKAFMSQVMPDNVNRVKLYRDDVPLFSRFQIEHQIESA
                     YNRQVTLPSGGAIVIDHTEALVSVDVNSGRATKGSDIEETALRTNLEAADEIARQLRL
                     RDLGGLIVIDFIDMESSKAQRDVENRLREALRFDRARVQTGKISRFGLMELSRQRLRP
                     ALAETNYITCPRCTGTGHIRSTESAALHILRILEEEAMKENTGAVHVQLPVDVASFLL
                     NEKRSEVQTIEIRHKVNLLLIPNIHLETPAHKIVRLRHDDLNQEDLAQPSYKMVEMPT
                     DMNAEAIAKDEAGKPRQEAAIRQFNPDQPAPIAPPAAASATPAPVAATGQTRKPGLLA
                     RFVSWLLGEPTSEEPAAPAVAETPRNASGRQQNARGNNRRRDGQSSRDGNRDGNRRGG
                     SNRGEPRQPRGAGRSAAATENGVESETKPRQENRERQQGRQRQNRQAAPAAIARPENE
                     TTTTAAELPPEAALESSAPKGDGDNAKGSGRGRRGRRGRRGEGRRNEENAAAVDAPEA
                     NASPLAPASRSETESAVDQEANVPVAAIENPETRVQATEPEIAETPSQQPAPVSAPEV
                     AEATVEAAPPVSTEATEAVTKTPAKPVETVTAAVPAIAAAATPAAAVPAPPAAAVDNT
                     IDLKETLADSGLVMIETSSAPARASVDEAPVKLGRPRKPTVTIADEPLKQVETGSN"
     gene            5184..6167
                     /locus_tag="VIS73_04925"
     CDS             5184..6167
                     /locus_tag="VIS73_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY5762528.1"
                     /translation="MTSSRKESVTYHEIDEGSDGQRLDNFLIRICKGVPKSHLYRVVR
                     SGEVRVNGRRCAVDYRLQCDDRVRVPPLRLPERLADVGGGIERAAALRTEIPILHEDE
                     RLLAVNKPAGLAVHGGSGVASGLIETLRVQRPQQPFLELAHRLDKETSGVLLLAKRRS
                     TLRRLHELFRSGSLDKRYLVLVAGRWTRGTAHVRIALGKTIDARGEKRVFADPDGQEA
                     YSIFRPLAVTENASLLEACIKTGRTHQIRVHLAHLGFPVLGDERYGDFTLNRALRPQG
                     LRRMFLHARALSLVHPDTMEALALYAELPADLRRFLVGIDGIREQIDGIEI"
     gene            6151..6807
                     /locus_tag="VIS73_04930"
     CDS             6151..6807
                     /locus_tag="VIS73_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY5762529.1"
                     /translation="MASKFDLIVFDWDGTLMDSAAAIVAAIGAACVDLGIEPPPEQRA
                     RHVIGLGLGDALRLAVPELETSRYDEMVAAYRRHYLAGDGDLRLFAGVDALLGELRER
                     EKVLAVATGKSRVGLDRALSTTGTAHRFHATRCADECFSKPHPQMLIELMDELGVAAE
                     RTLMVGDTTHDLQMAAAAGTPALAVSYGAHPADTLAQHRALAQLDSPAALAGWLLENA
                     "
     gene            6786..7136
                     /locus_tag="VIS73_04935"
     CDS             6786..7136
                     /locus_tag="VIS73_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY5762530.1"
                     /translation="MAAGKRLICDSAALVDGGVGLRFMVIRYGREEPAFAIRFKGAAY
                     AYLNRCGHTPIELDWQAGEFFDLERRWIICATHGAIYHPATGECVTGRCAGRGLVALQ
                     LTEEEGHVYVVETE"
     gene            7117..8097
                     /locus_tag="VIS73_04940"
     CDS             7117..8097
                     /locus_tag="VIS73_04940"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEY5762531.1"
                     /translation="MWSKRNSDSADAESAAGNVEGRAQLLLAQVTREALREQRLRRRW
                     GIFFKFLTFGYLLAIVLIVVDIGEPEELVDGQHTALVDLQGVISASADASSENLVDAL
                     QRAFEHKKTKGVVLRINSPGGSPVQSGIVHDEILRLRKANPEKQFHVVVEDICASGGY
                     FVASAADRIYVNRASIVGSIGVLIDGFGFTGAMDKLGVERRLLTAGENKGFLDPFSPQ
                     EGRQRAHAQVLLDDIHRQFIEVVRKGRGSRLKDNPDVFSGLMWTGERSIEMGLADELG
                     TVESVARDVIEAERIVDFSVKPGFGERLARRFGAGLGESLAAVLSGQLIR"
     gene            complement(8109..8825)
                     /locus_tag="VIS73_04945"
     CDS             complement(8109..8825)
                     /locus_tag="VIS73_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY5762532.1"
                     /translation="MPSASLFLIPVPLGDLPPESSLAAATIAEVHSLDHFAVERAKTA
                     RAHLRAFAHPKPLQSLAMVELDGAQTDESIRQALAWLEAGYDVGLMSEAGCPAVADPG
                     SRLVARCHERGIRVRPLVGPSSLLLAIMASGLNGQKFAFHGYLPAKPLPRAEAIRDLE
                     KRSQADACLQLFIETPYRNGTLLQSLLAECRESTRLCVAIDLTLPAEFVRTATISDWR
                     QHKALPPVDKRPTVFLLQAG"
     gene            complement(8829..>9370)
                     /locus_tag="VIS73_04950"
     CDS             complement(8829..>9370)
                     /locus_tag="VIS73_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEY5762533.1"
                     /translation="RELLARLSIPFAVLSPDIDETPRAGETPRELALRLAGEKARAVA
                     RSHSAGLIIGCDQVAHIGTRVFGKPGTHQNAARQLAEMSGQTVVFESALCLLDASTGR
                     MQIDSIPTEITFRTLSAPEIENYLHRDQPYGCAGSAKLESLGIALIERMRSDDPTAII
                     GLPLVRLCRMLAAENVAVL"
BASE COUNT         1561 a         2853 c         3119 g         1837 t
ORIGIN      
        1 gcatctcgct ctttgacgat gaagtcgaac gcctcgatct cttcgatccc ttgaccgggc
       61 aaatcgagcg ccggctgtcg cggttcacgg tctatccttc gagccactac gtcacgccac
      121 ggtcgacagt gctccgtgcc atcgaggcga tcaagcagga gctgcgtgaa cggatcgcgt
      181 tcttcgacgg ccagaccaag ctcatcgaag cgcagcggat cgcccagcgg acgcgctttg
      241 acctggagat gctcgacgaa ctgggattct gcaagggaat tgagaactac tcgcggcatc
      301 tctcagggcg ctccgccggc gaaccgccgc cgaccctgat cgattacctg cccgacgacg
      361 cactgatatt catcgacgaa tcgcatgttt caattcccca ggttggtggc atgttcagag
      421 gcgacctgtc gcgcaagcag aatctcgtcg actacggctt tcgcctgccc tcggccctcg
      481 acaatcggcc gctgcgcttc gacgaattcg aacggcgact gcgacaggcg atcttcgtct
      541 cggccacgcc gtccgactac gaggcgcagc atgccggtgc cgttgtcgag caactggtcc
      601 ggccgaccgg gctgatcgat cccgatctcc tggttcgtcc ggcggcgaat caggtcgacg
      661 acctactgca cgaaatccgt cggcgcacgg ctgtcggcga gcgcgttctg gtgacgacgc
      721 tgaccaagcg catggccgag gatctgaccg actatctggc cgacaacggt gtccgtgtgc
      781 gttacctcca ctcggacatc gataccgttg aacgcgtcga gatcattcgg gacctgcggc
      841 tcggagagtt cgatgtgctg gtcggcatca acttgttgcg agaggggctc gatattcccg
      901 aagtgtcgct ggtggcgatc ctcgacgccg acaaggaggg gtttctgcgc tcggcgcgct
      961 cgctgattca gaccatcggc cgcgccgcgc gccacctcaa tggcaccgcg attctctacg
     1021 ccgatcgcgt gaccgattcg atgcggcgcg cgatagacga gaccgatcgg cgtcgccgga
     1081 agcaggtcga attcaacacc ctgaattcga tcacgccgaa gagcgtgtcg aagcgcgtca
     1141 aggacatcat cgatggtatc tacgatccac aggccgagcg aacggcgatg gcgctggccg
     1201 aagagcgcgt ggcctatgca acgatggacc aggcctcgct cgcccgcgaa atccgcgagc
     1261 tcgaacggca gatgagcgaa cacgcgcgaa acctcgagtt cgaacaggcc gccgcggccc
     1321 gcgatcgatt gtttcgcctg cgcgagagcg cgctgggttt ggcgacctca ggcgccggcg
     1381 gaatggccaa gggcgcggca ccgcaaaaac agccacgccg ccgccggggc aggtgaaggc
     1441 gcagcgccgc tgtcgtagaa tatccgcatg gtcaggaacg caccgacaat ccgcaagcag
     1501 ggtttgccga gggctgccac gcagacgacg cgcgaggttc gtgtgctctt cgtttgcatg
     1561 ggcaatatct gtcgctcgcc gaccgccgag gccgtgttgc gcaaggccct caaggaacat
     1621 gggatcgacg accgggtcga ggtcgattcg gccggtacgc atggctatca cgtcggtgag
     1681 ccacctgatc cgcgcacgca gcgggccgcg gaaagccgcg gctacgacct gtcctcgatc
     1741 cgcgcgcgca aggttgcgcc gcaggatctc gaatacttcg acctggtcct cgcgatggat
     1801 cgcagcaacc tcgagaatct gcagcggatc tgcctgcccg atttcggcga tcgactcggc
     1861 ttgtttatgc cctactcacg cgctttcgat gacgacgaag ttccagaccc ctactatggc
     1921 ctggggcatg gtttcgatct ggtcctcgac atgatcgagg acgccagtgc cgggctgatc
     1981 gtcgaaatta aaaaacggct cgccgccaag tagcgggagc cgttctttcg ggaagcgcga
     2041 acgccctagt tcgatccggt ctcgacctgc ttgagcggct cgtcggcgat cgttaccgtc
     2101 ggcttacgcg gcctgccgag cttgaccggc gcttcgtcga ccgaggctct ggccggcgcg
     2161 gaactggttt cgatcattac cagacccgaa tcggcaagtg tttccttgag atcgatcgtg
     2221 ttgtcgactg ccgccgccgg aggcgccgga accgctgctg cgggggtcgc cgctgctgca
     2281 atggccggaa ctgccgcagt gactgtttcg acaggtttgg ccggcgtctt ggtcacggct
     2341 tcggtggctt cggtgctgac tggcggtgca gcttccacgg tggcttcagc gacttcggga
     2401 gcgctgacgg gggccggctg ctgactcggc gtttcggcga tctcgggttc ggtagcctga
     2461 acgcgagttt ccgggttttc gattgcggcg accggaacgt tagcttcctg gtctacagcg
     2521 ctctcggtct ccgaccggga cgccggtgcg agcggcgagg cattggcttc cggggcgtcg
     2581 actgccgcag cgttctcttc gttgcgacgt ccttcgccgc gacgaccgcg acgaccgcga
     2641 cggccacggc ctgatccctt ggcgttgtct ccgtcgccct ttggtgctga cgactccagc
     2701 gcggcctcgg ggggaagctc ggccgctgtt gttgtcgttt cgttttccgg ccttgcgatg
     2761 gcagcgggag ccgcctgacg attctggcgt tggcgaccct gctggcgttc gcgattctcc
     2821 tggcgtggct tggtttcgct ctccacgcca ttttcagtcg ctgccgctga ccgtcccgct
     2881 ccgcggggtt ggcgcggctc gccgcggttg ctgccgccgc ggcgattgcc gtcgcggtta
     2941 ccgtctcggc tgctctggcc atcacgccgg cggttgttgc cacgggcgtt ctgctgtcgt
     3001 cctgacgcgt tgcgcggtgt ttcggcaacg gcgggcgcag ccggttcctc gctagttggc
     3061 tcgccgagca gccaggagac aaagcgtgcg agaaggcccg gcttgcgggt ctggccggtg
     3121 gccgcaaccg gcgccggcgt ggcgctcgcg gccgcaggcg gcgcgatagg cgcgggctga
     3181 tcggggttga actgacggat cgccgcttcc tggcggggct ttccggcctc gtccttcgcg
     3241 attgcttcgg cgttcatgtc ggtcggcatt tcgaccatct tgtacgacgg ctgtgccagg
     3301 tcttcctggt tcaggtcgtc gtggcgcagg cgaacgatct tgtgcgccgg cgtctcgaga
     3361 tggatgttcg ggatcagcag cagattgacc ttgtggcgga tctcgatggt ctggacttcg
     3421 ctgcgcttct cattgagcag gaaggaggcg acgtcaaccg gcaactgcac gtgcacggcg
     3481 ccggtgttct ccttcatcgc ttcttcttcg aggatgcgca gaatgtgcag cgcagccgat
     3541 tcggtcgagc ggatgtggcc ggtaccggta cagcgcgggc aagtgatgta gttggtttca
     3601 gccaatgccg ggcgcaggcg ctgacgcgac agctccatca ggccgaagcg gctgatcttg
     3661 ccagtctgta cgcgggcacg gtcgaaacgc agcgcttcgc gcaggcggtt ctcgacatcg
     3721 cgctgtgcct tgctactttc catgtcgatg aaatcaatga cgatcagacc gccgaggtcg
     3781 cgcagacgca attggcgggc gatttcatca gccgcctcaa ggttggtgcg cagcgcggtt
     3841 tcttcgatgt cgctgccttt agtggcacgg cccgagttga cgtcgaccga gaccagcgcc
     3901 tcggtatggt cgatgacgat cgcgccgccg gacggtagcg tgacctgccg gttgtaggcg
     3961 gattcgatct ggtgttcgat ctggaaacgc gagaacagcg gaacgtcgtc gcggtagagc
     4021 ttgacccggt tcacgttgtc cggcatcacc tggctcatga aagccttggc ctgctcgtag
     4081 atgtcctcgg tgtcgatgag gatttcgccg atttcaggct ggaagtagtc gcggatcgcg
     4141 cggatcacga ggctgccttc ctgatagatc agcagcggtg ccttgagttc gccggcgacg
     4201 ccgtcgatgg ccgaccacag ctgctgcagg tagttgaggt cccactgaag ttcttcaagc
     4261 gcccggccga tcgctgcggt acgggcaatg acgctcattc cgtccggtac ttcgatctgc
     4321 gccagaattt cgcgcagttc ggagcgctcg tcaccttcga cgcgacgcga aacaccaccg
     4381 ccgcgcgggt tgttcggcat cagcaccaga tagcggccgg ccagcgagac ataggttgtc
     4441 agcgccgcac ccttgttgcc gcgttcgtcc ttgtcaactt ggacgacgag ttcctggcct
     4501 tctttcagcg cttcgttgat cttggcgcgg ctggcatcga cgccgggctt gaagtaactg
     4561 cgggagactt ccttgaaagg caggaagccg tggcggtcgg cgccgtaatc cacaaaggcg
     4621 gcttcgaggc tgggctcgat gcgggtaatg acacccttgt agatgttgct cttgcgctgc
     4681 tctttggcag ccgactcgat gtcgaggtcg atgagctttt gcccgtcgac gatggcaacg
     4741 cgcagctctt cagcctgcgt tgcattgaac agcattcgtt tcatttgtcc gtgactcccg
     4801 cgcgcgtctg cacgggtgga cgactccgct cgtggtctcg gtaagaccgc gagaaaaatt
     4861 agtggctgtt atcaggcagt gacatgactg ggttggcgac taagttcggt ttcgtcatgc
     4921 gccagccggc ttgggttgtt cggctggcgc gcagtatccg ttgttcggtg ctggacgcgc
     4981 gcaattcaag tagtatcgct actcccggtg agcgacataa ccggcttcaa ctgcctcggg
     5041 atggcctgaa ggattgggtc aggcggggcc tcgaatctct gcccgaaggt taccgccgcg
     5101 ctccgaatcg agcgtgcggc cctgcggtct tatgacattc agctttcatt gcccgggcgt
     5161 aaagcgggaa cagtgtattc gatatgacgt cctcgcgtaa agaatccgtc acttaccacg
     5221 agatcgacga aggatcggac ggtcagcggc tggacaattt cctgatccga atctgcaagg
     5281 gcgtgcccaa gagccacttg tatcgggtgg ttcgctcggg tgaggtgcgc gtaaatgggc
     5341 ggcgctgcgc cgtcgactat cgactgcagt gcgatgatcg ggtaagggtg ccgccgctgc
     5401 ggctacctga gcggctagct gatgtcggcg gcggcatcga gcgagccgcc gcgctgcgga
     5461 ccgagattcc gatcctgcac gaggacgagc gcctgctcgc ggtcaacaag cccgccggcc
     5521 tcgctgttca cggcggcagc ggggttgcct ccgggctcat cgaaacattg cgtgtgcagc
     5581 ggccgcagca gccctttctc gaactggcgc atcggctcga caaggaaaca tcgggtgtgt
     5641 tgttgctggc caagcggcgt tcgacgctgc gtcgcctgca tgagctgttt cggtcgggat
     5701 cgctcgacaa gcgctacctc gtgctggtcg ccgggcgatg gacccgtggc accgcgcacg
     5761 tacggatagc gctgggaaag accatcgatg cacgcggcga gaaacgcgtt tttgccgacc
     5821 ccgacgggca agaggcctat tcgatctttc gtccgctggc agtcacggaa aatgccagcc
     5881 tgctcgaagc ctgcatcaag accggacgga cccaccagat tcgcgttcat ctcgcgcatc
     5941 ttggcttccc cgtcctcggc gacgagcgct acggcgactt cacgctgaac cgggcgctgc
     6001 ggccgcaagg cctgcgccgc atgtttctcc atgcgcgcgc cttgtcgctg gttcatcccg
     6061 atactatgga agccttggcg ctatacgctg aattgccggc tgacctgcgt cgtttcctcg
     6121 tgggaatcga cggcatccgc gagcagatag atggcatcga aatttgacct gatcgttttt
     6181 gactgggacg gcacgctgat ggactcggcg gcggcgatcg tcgccgcaat tggcgctgcc
     6241 tgcgtcgact tgggaatcga gccgccgccc gagcagcgcg cgcgccacgt tatcgggctc
     6301 ggcctcggcg atgcgctgcg tctggctgtt ccggaactgg agacctcgcg ctatgacgag
     6361 atggttgcag cgtatcgccg gcactacctg gcgggcgacg gggaccttcg cctgttcgcc
     6421 ggcgtcgacg cgctgctcgg cgaactgcgc gagcgcgaga aggtgctggc ggtggcgacg
     6481 gggaaaagcc gggtcggtct cgatcgtgcc ctgtcgacga ccggaacggc gcaccgtttc
     6541 catgcgacac gctgcgccga cgagtgcttc tcgaagccgc atccgcagat gctgatcgaa
     6601 ctgatggacg aactgggtgt ggcggccgag cgcacgctga tggtcggcga caccacccac
     6661 gatctgcaga tggccgcagc ggccggaacg ccggcgttgg cggtcagcta cggcgcgcat
     6721 ccggccgata cattggcgca acaccgggcg ctagcacaac tcgactcgcc cgcggcgctt
     6781 gccggatggc tgctggaaaa cgcctgatct gcgatagcgc agcgctggtc gacggtggcg
     6841 tcggcctccg tttcatggtc atccgctacg gtcgcgaaga gccggctttc gcgattcgct
     6901 tcaagggcgc ggcctatgcc tacctgaacc gatgtggcca taccccgatc gaactcgact
     6961 ggcaggcggg agaattcttc gatctcgaac gacgctggat catatgcgcc acgcatggcg
     7021 cgatttacca cccggcgacg ggcgaatgcg tgaccggccg gtgtgccggg cgcggtctcg
     7081 tcgccctgca actgacagag gaagaggggc acgtttatgt ggtcgaaacg gaatagcgat
     7141 tccgccgatg cggaatcggc agccggcaat gttgaggggc gggcgcagtt gctgctggcc
     7201 caggtcacgc gcgaggcgct tcgcgagcag cgtctgcggc gacggtgggg aatcttcttc
     7261 aagttcctga ccttcggcta cctcctcgcc atcgtgctga tcgttgtcga tatcggtgaa
     7321 cctgaggaac tcgtcgatgg gcagcacacc gctctggtag acctccaggg ggttatttcg
     7381 gcttcagccg acgccagttc ggagaatctc gtcgacgcgc tgcaaagggc cttcgagcac
     7441 aagaagacca aaggtgtcgt gctgcgcatc aacagtccgg ggggcagccc ggttcagtca
     7501 ggaattgttc acgacgaaat cctgcgactg cgcaaggcga atccggaaaa gcaattccat
     7561 gtcgttgtcg aagacatctg tgcctccggc ggttatttcg ttgcgtcggc cgccgaccga
     7621 atttacgtca accgcgccag catcgtcggc tcgatcggcg tgctgatcga cggcttcgga
     7681 tttaccggcg ccatggacaa gctcggcgtc gagcggcgcc tgctgacggc cggagagaac
     7741 aagggttttc ttgatccgtt ctcgccacag gagggtcggc agcgcgccca cgcgcaagtt
     7801 ctgctcgacg acatccatcg gcagttcatc gaggttgtgc gcaagggccg cgggagccga
     7861 ctcaaggaca acccggacgt cttttccggg ctgatgtgga ccggcgagcg cagcatcgaa
     7921 atgggcctgg ctgatgaact gggaaccgtg gaaagcgtag cgcgggatgt catcgaagcc
     7981 gaacgcatcg tcgacttcag cgtcaagcca ggattcggcg agcgcctggc gcggcgcttt
     8041 ggtgccgggc tcggtgagtc gctagccgca gtgctgagtg gccagctgat ccgctgacgc
     8101 gtcgccgctc aaccggcctg cagcaggaat acggttgggc gcttgtcgac tggcggcagt
     8161 gccttgtgct gccgccagtc ggaaatcgtg gcggtgcgca cgaattcggc gggcagcgtc
     8221 agatcgatgg cgacgcacag tcgcgtcgac tcccggcatt cggcaagcag gctctgcaac
     8281 agcgttccat ttcgatacgg cgtttcgatg aagagctgca gacaggcatc ggcctgtgag
     8341 cgcttctcca gatcgcgaat cgcctccgcg cgcggcagcg gttttgccgg caggtagccg
     8401 tgaaaggcga atttctgacc gtttaacccg gaggccatga ttgcgagtag cagcgatgat
     8461 ggcccgacca gcgggcgtac gcgaatcccg cgttcgtgac atcgcgcgac taggcgactg
     8521 ccagggtcgg cgactgccgg gcagccggcc tcggacatca ggccgacgtc atagccagcc
     8581 tcaagccagg ctagcgcctg acgaatcgat tcgtcggtct gcgcgccgtc gagctcaacc
     8641 atcgccagcg attgcagggg tttcgggtga gcgaaggcgc gcaggtgtgc gcgcgccgtc
     8701 ttggcccttt caacggcgaa gtggtcgagc gaatggacct cggcgattgt tgcggccgcg
     8761 agggaggatt cggggggcag gtcaccaagc ggtaccggga tcaggaacag cgatgcggac
     8821 ggcatcgctc agagcaccgc gacgttttcg gcagcgagca tgcggcaaag acggaccagc
     8881 ggcaggccga tgattgcggt cgggtcgtcg ctgcgcatgc gctcgatcag cgcaatgcct
     8941 aggctttcca gcttggcgct gcccgcgcag ccatagggtt gatcgcggtg caggtagttc
     9001 tcgatttcgg gcgcgctcag ggtgcggaag gtgatttcgg tcgggatcga gtcgatctgc
     9061 atccgtcccg tgctcgcgtc gagcaggcac agggctgatt cgaacacgac ggtctggccc
     9121 gacatttcag cgagctggcg agccgcgttc tggtgggtgc cgggcttccc gaagacccgg
     9181 gtaccgatgt gggcgacctg gtcgcagccg atgattagac cggccgaatg gcttcgggcg
     9241 acggcccgcg ccttttctcc ggccaagcgc aaggcaagct cgcggggtgt ttcgccggcc
     9301 cggggggtct cgtcgatatc gggtgagagg accgcgaagg ggatgctcag gcgggcaagc
     9361 agttcacggc
//
LOCUS       DATDAA010000152         7071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_10933,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000152 DATDAA010000000
VERSION     DATDAA010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10933"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VIS73_04955"
     CDS             <1..366
                     /locus_tag="VIS73_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY5762534.1"
                     /translation="LATQALYGHLLAGGVRIYEYWHSYLHAKVAVIDESWATVGSSNI
                     DPFSLLMAKEANLFSVDRDFATALRGHLDAAIANDAREIVRTHWQRFSPLQRALRWTS
                     YQLIRLAVGVTGYLRPGEE"
     gene            complement(342..1301)
                     /gene="gluQRS"
                     /locus_tag="VIS73_04960"
     CDS             complement(342..1301)
                     /gene="gluQRS"
                     /locus_tag="VIS73_04960"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEY5762535.1"
                     /translation="MSIGSEPSLGPKRYRGRFAPSPTGPLHLGSLVAAVGSWLDARAA
                     GGEWLLRIEDVDVGRCQPGAEDSILRTLEACGLTWDGPVVRQSERTEAYAAALDALRA
                     AGEVYLCACSRKAIEALTDRRAADGALIYPGTCRGGLATGVEARAWRLRVPAAAVEFD
                     DRLLGRCSQRLAEEVGDCVLWRADGLAAYQLAVVVDDAEQAITDVVRGADLFDATPRQ
                     IHLQRCLHVPTPRYAHLPVMANEAGEKLSKQTLAPAIEAAAAGVAITAALEFLGHRTP
                     VYLRGAPPADQLAWARGAWSLVRVPARNTIAVNAAIYSSPGRR"
     gene            1405..3489
                     /locus_tag="VIS73_04965"
     CDS             1405..3489
                     /locus_tag="VIS73_04965"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY5762536.1"
                     /translation="MHKRPSPRSQFRHACRHTAAAALAVVALVAAPTATAAQNRIALV
                     VGNADYLTAPLKNPQNDARDIAEKLKQVGFDVVLRENLKIRDIGGALREFRARLSPGD
                     EALFFYAGHGLQVKGINYLPAVDAEIKTEEDLPFQSLNVNQVLEIMDDARTRLNLVFL
                     DACRNNPYSRRFRSTAGGLAKINAPSGTLISFATRPGSIAADGEGRNGLFTEHLLQQM
                     DVPNLPIESVLKRVVSGVKQATAGAQEPWLEGSIEGEFYFVSAPKAAPAATLAAAAPT
                     LVVARDAEDALWAAIERAPKLGDYEIYLKTYPKGKYVALATSRMAMMREAHQARSDEE
                     AAWAAAERTQSAAAYQAYLAKYPAGLFAPLAGARLDRLRVEESVAADDSRKREFEAWD
                     SAQRERTPTALLAFLDRFPASEFAPPARALLGELKLIAALKPGQPFRDCPNCPELVIV
                     PPGSFEMGDPDSGGGLSFVPFVGGSEGPRHTVTISQPFAAGRHEVTFAEWDACVADKA
                     CTHRPEDHGWGRDRRPVVGVSWVDAQQYLAWLSRRTGHSYRLLTEAEWEYAARAGAAT
                     SYPWGHAIGSKLANCKGCGSQWDNQQTAPVGSFAANAFGLHDVLGNASDWVEDCSVSG
                     YEEAPTDGSARIKDGCGSRVLRGGALYDRVKNVNVFRRFSDGADDREMWYGFRVARNV
                     TATDAARSAAKQ"
     gene            complement(3584..3757)
                     /locus_tag="VIS73_04970"
     CDS             complement(3584..3757)
                     /locus_tag="VIS73_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762537.1"
                     /translation="MAKPNYQFEKRQRDLAKKAKQEEKRRQKLAAKAAAAEAPSPQPN
                     ESGADPVGGEASS"
     gene            complement(3958..5049)
                     /gene="ychF"
                     /locus_tag="VIS73_04975"
     CDS             complement(3958..5049)
                     /gene="ychF"
                     /locus_tag="VIS73_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY5762538.1"
                     /translation="MSLQCGIVGLPNVGKSTLFNALTKAGIQAENYPFCTIEPNVGIV
                     EVPDGRLAQLAAIVKPQKVQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVH
                     VVRCFADDNVVHVSGRIDPIADIETIDTELALADLATAEKALNRYRKPAQAGDKDAKI
                     LVAALEKCVAQLDSGKPVRALDLSKEERLNIKPFCFITAKPVLYAANVAEDGFRDNPH
                     LATVEAHAKAEGAEVVAICAAIESEIAGLEDDEKEMFLADMGLEEPGLNRLIRAGYQL
                     LGLQTYFTAGVKEVRAWTIHAGDTAPQAAGVIHTDFEKGFIRAQTIAFDDFIQFKGEQ
                     GAKEAGKMRAEGKDYVVKDGDVLNFLFNV"
     gene            complement(5046..5639)
                     /gene="pth"
                     /locus_tag="VIS73_04980"
     CDS             complement(5046..5639)
                     /gene="pth"
                     /locus_tag="VIS73_04980"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY5762539.1"
                     /translation="MSKLRLIVGLGNPGAEYEATRHNVGFWLVEALARELRTTLAPQG
                     KFFGNAGRSGELWLLCPSTFMNRSGQSVAALARFYKIEAEEILVVHDELDLPPGSMRV
                     KRGGGTAGHNGLKDIQAQMGSADFWRLRLGIGHPRDSQSHQGVADYVLHAPRKEEQDA
                     IEAAIARCLSVWPKLAGGDFAGAQAVLHRAENHKEET"
     gene            complement(5724..6386)
                     /locus_tag="VIS73_04985"
     CDS             complement(5724..6386)
                     /locus_tag="VIS73_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEY5762540.1"
                     /translation="MQFELNAQSRTLQGTGASRRLRHAGKVPGIVYGGTAQPTPVEVD
                     HNELLLSLRKEAFHSSVITLVLDGKKESVLLRDVQAHAYKKLVLHVDFQRVDAKKELH
                     IAVPLHFINGDIAPGVKLGGGIVSHVMTELEVKCLPADLPEFLEVDLKDLVAGHSLHV
                     DEIKLPKGVKAVLHGGDNPVVATILTVRGDAAADEAEAADAATAAAASAAATPAATPP
                     KA"
     gene            complement(6438..>7071)
                     /locus_tag="VIS73_04990"
     CDS             complement(6438..>7071)
                     /locus_tag="VIS73_04990"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY5762541.1"
                     /translation="RVPIAAKLVASMLTTAGIDRVLTMDLHAEQIQGFFDMPVDNIYG
                     LPILLGDIWRQNYPELIVVSPDVGGVVRARSLAKRLECDLAIIDKRRPKANVSEVMNI
                     IGDVEGRTCVIIDDIVDTAGTLCKAAQALKANGAAKVMSYCTHAVLSGSAVERISNSA
                     LDELVVTDTIPLRDEAAACPRIRVLSVADLLAETIRRICNEESVSSLFLE"
BASE COUNT         1175 a         2383 c         2342 g         1171 t
ORIGIN      
        1 ctggccacgc aggcgctcta cggtcacctg ctcgccggcg gcgtgcgcat ctacgagtac
       61 tggcacagct acctgcatgc caaggtcgcg gtcatcgacg agtcctgggc gacggtcggt
      121 tcctcgaaca tcgacccgtt cagcctgctg atggccaagg aagccaacct cttcagcgtc
      181 gaccgcgatt tcgccacggc gctgcgtggc cacctcgacg cggccatcgc caatgacgcc
      241 cgcgagatcg tgcgcacgca ctggcaacgc ttctcgccgc tgcaacgcgc actgcgctgg
      301 acgagttatc agctaatccg gctcgccgtc ggcgtcaccg gctacctgcg ccccggcgag
      361 gagtagatcg cggcgttaac ggcgatcgtg ttccgcgccg ggacccgcac caacgaccag
      421 gcgccgcgcg cccaggccaa ctggtcggcc ggcggcgcac cgcgcaggta cacgggcgtc
      481 cgatggccaa gaaattcgag cgcggcagtg atcgccacgc ccgcagccgc ggcttcaatc
      541 gccggcgcca atgtctgctt agagagtttc tcgcccgctt cgttggccat caccggcagg
      601 tgcgcgtagc gcggcgtcgg cacgtgcagg cagcgctgca ggtggatctg tcgcggcgtc
      661 gcgtcgaaaa ggtcggcgcc gcggacgaca tcggtgatcg cctgttcggc atcgtcgacg
      721 accacggcga gttgataggc agccaggcca tcggcgcgcc agagcacgca atcaccaacc
      781 tcttcggcga gccgctgcga acaacgcccg agcaggcggt cgtcgaattc cacggcggcc
      841 gccggcacgc gcaggcgcca ggcgcgcgcc tcgacgccgg tggccaggcc accgcggcag
      901 gttcccgggt agatcagtgc gccatccgcg gcacggcgat cggtcagcgc ctcaattgcc
      961 ttgcggctgc aggcgcagag gtagacctcg ccggcggcgc gcagcgcatc gagcgccgcc
     1021 gcataggcct ccgtccgctc gctctggcgg accacggggc cgtcccacgt caggccgcaa
     1081 gcttccagcg tgcgcaggat cgaatcctcg gcaccgggct ggcaacgacc cacatcgacg
     1141 tcttcgatcc gcaacagcca ttcgccgccg gccgcgcgcg cgtcgagcca gctgccgacg
     1201 gcggcaacca gcgaaccgag gtggagcgga ccagtcggcg atggcgcgaa tcgcccacga
     1261 tagcgcttcg gaccgagaga aggctcggac ccgatgctca tcggcgggcg gaacaatgcg
     1321 gcagccgttg cggcatttgt cttgtcattg gtggaatcgc ggggcagact gaatttccga
     1381 tccagtttgc ccttgtcgcc agcgatgcac aaacgcccca gcccgcgcag ccaattccgc
     1441 cacgcctgcc gtcacacggc cgccgccgcg ctcgcggtag tcgccctggt tgccgcgccg
     1501 accgccaccg ccgcgcagaa ccgcatcgcg ctggtggtcg gcaacgccga ctacctcacc
     1561 gcgccgctga aaaacccgca gaacgacgcg cgcgacatag cggaaaagct caagcaggtc
     1621 ggcttcgacg tggtgttgcg cgagaacctg aagatccgcg acatcggcgg tgcgctgcgc
     1681 gagtttcgcg cccggctgtc gcccggcgac gaggcgttgt ttttctacgc aggccatggc
     1741 ctgcaggtga agggcatcaa ctacctgccg gccgtcgacg ccgaaatcaa gaccgaggaa
     1801 gacctgccct tccagagcct taacgtcaat caggtgctgg agataatgga cgacgcgcga
     1861 acgcggctga acctggtctt cctcgatgcc tgccgcaaca acccgtattc gcgccgcttc
     1921 cgctcgaccg ccggcggcct cgccaagatc aacgcgccat cgggcacgct gatctcgttt
     1981 gcgacgcgcc caggcagcat cgccgccgac ggcgagggac gcaacggcct attcacagag
     2041 cacctgctgc aacagatgga cgtgccgaac ctgccgatcg agtcggtgct gaaacgcgtg
     2101 gtcagcggcg tcaagcaggc gaccgcgggc gcacaggagc cctggctcga aggcagcatc
     2161 gaaggcgagt tctacttcgt cagcgcgccg aaagcggctc ccgcggcgac gctggccgcg
     2221 gctgcgccga ctctggtcgt cgcccgcgac gccgaagatg cgctgtgggc agcgatcgaa
     2281 cgcgcaccca agctcggcga ctacgagatc tatctcaaga cctaccccaa gggcaagtac
     2341 gtggcgctgg ccacctcgcg catggccatg atgcgcgagg cacaccaggc gcgcagcgac
     2401 gaggaagccg cctgggcagc cgctgagcgc acgcaatcag ccgccgcgta tcaggcctac
     2461 ctagcgaaat acccggccgg tctctttgcg ccactggcgg gcgcacgact cgatcgactg
     2521 cgcgtcgagg aatcggttgc cgccgacgac tcgcgcaagc gcgaattcga agcctgggac
     2581 tccgcccagc gcgagcgcac gccgacagcg ctgctggcct tcctcgaccg ttttcccgca
     2641 tccgaattcg cgccgccggc gcgcgccttg ctcggcgagt tgaagctaat cgcagcgctc
     2701 aaacccggcc agccgtttcg agactgcccg aactgcccgg agctggtcat cgtcccgccg
     2761 ggcagcttcg agatgggcga ccccgatagc ggtggcggct tgagcttcgt tcccttcgtc
     2821 ggcggctcgg agggtccacg gcatacggtg accatcagcc agccattcgc tgccggccgc
     2881 catgaggtga ccttcgccga atgggacgcc tgcgtcgccg acaaggcctg cacccaccgc
     2941 cccgaagacc acggctgggg ccgcgaccgg cgccccgtcg tcggcgtctc ctgggtcgac
     3001 gcgcagcaat atctcgcctg gctgagtcgt cgcaccggcc acagctaccg cttgctgacc
     3061 gaggccgaat gggaatacgc ggcgcgcgcc ggcgccgcaa ccagctatcc ctgggggcac
     3121 gcgatcggca gcaagcttgc caactgcaaa ggttgtggca gccagtggga caaccagcag
     3181 accgccccgg tcggcagctt cgccgccaac gccttcggcc tccacgacgt gctcggcaac
     3241 gccagcgact gggtcgagga ctgctccgtt agcggctacg aagaggcccc caccgacggc
     3301 agcgcaagga tcaaagacgg atgtgggtcg cgcgtgctgc gcggcggcgc gctctacgac
     3361 cgcgtgaaaa acgtcaacgt ctttcgccgc ttctccgacg gcgccgacga ccgcgagatg
     3421 tggtacggct tccgcgtcgc gcgcaatgtg acggcgacgg acgcggcccg gtcagcggcg
     3481 aagcagtagg cagcgtagcg cggtcggtct tctggccccg ccgctcaagg ccaagggcct
     3541 gtggaggatc ggggccggct cgctcgacag cggcgcccgt gccctagctg cttgcctccc
     3601 cgccgacggg gtcagcgccg gactcgttcg gctgtgggga gggggcttct gccgcggcgg
     3661 ccttggccgc gagcttctgc cggcgcttct cttcctgctt ggccttcttg gcgagatcgc
     3721 gctgacgctt ttcgaactgg tagtttggtt ttgccatggc ccgagggtaa ccggaatcgg
     3781 ggttcgaatc acccggtttt cactcggatc gacaaattgt ctgggcaatc ggcccggcga
     3841 ggccgtcgcc gcgacaatgc acggcgtagc gcgccccggc cggcgggggc gccagccccg
     3901 attgctgcgg cagcgcgaac gatccggcca tcgcagggcg gtggccgctc gttccgatca
     3961 gacgttgaac aggaagttca gcacgtcgcc gtccttgacg acgtagtcct tgccttcggc
     4021 gcgcatcttg ccggcttcct tggcgccctg ctcgcccttg aactggatga agtcgtcgaa
     4081 ggcgatcgtc tgcgcgcgga tgaagccctt ctcgaagtcg gtgtggataa cacccgccgc
     4141 ctgcggcgcg gtgtcgccgg cgtgaatggt ccaggcacga acttccttga ccccggccgt
     4201 gaagtaggtc tgcaggccga gcagctgata gccggcgcgg atcaggcggt tgagccccgg
     4261 ttcttccagt cccatgtcgg cgaggaacat ctccttctcg tcgtcttcga gtccggcgat
     4321 ctccgattcg atcgccgcgc agatggcaac gacttcggcg ccctcggcct tggcgtgcgc
     4381 ctcgacggtg gccagatgcg ggttgtcccg gaagccatcc tcggcgacgt tggcggcgta
     4441 gagcacgggc ttggcggtga tgaagcagaa gggcttgatg ttcagccgct cctccttgga
     4501 gagatcgagc gcgcgcaccg gcttgccgga atcgagctgg gcgacgcatt tttcgagcgc
     4561 ggcgactagg atcttcgcat ccttgtcgcc ggcctgcgcc ggcttgcgat agcggttgag
     4621 cgccttctcg gcggtggcga ggtcggctag cgcaagttcg gtgtcgatcg tctcgatgtc
     4681 ggcgatcggg tcgattcggc cggagacgtg gacgacgttg tcgtcggcga agcagcgcac
     4741 gacgtggacg atcgcgtcgg tctcgcggat gttggcgagg aactggttgc cgaggccctc
     4801 gcccttcgag gcaccggcaa cgaggccggc gatgtcgacg aattcgacga ttgccggctg
     4861 caccttctgc ggcttgacga tcgccgcgag ttgcgcgagg cgtccgtccg gcacctcgac
     4921 gatgccgacg ttcggctcga tcgtgcagaa agggtaattc tcggcctgga tcccggcctt
     4981 ggtcagcgca ttgaacagcg tcgacttgcc gacgttgggc aggccgacga ttccgcattg
     5041 gagactcatg tttcttcctt atggttttcg gcgcggtgca agaccgcctg cgcgccggcg
     5101 aaatcgccgc ccgcgagttt gggccagacc gacagacaac gggcgatcgc agcctcgatg
     5161 gcgtcctgtt cttccttgcg cggcgcgtgc agcacgtagt cggcgacgcc ctgatgcgac
     5221 tgcgagtcac gcgggtggcc gatgcccagg cgcaggcgcc aaaaatcggc actacccatc
     5281 tgcgcctgaa tgtccttcag gccgttgtgc ccggccgtgc cgccgccccg cttgacgcgc
     5341 atggaacccg gcggcaggtc gagctcatca tggacgacga ggatctcttc ggcttcgatc
     5401 ttgtagaagc gcgccaacgc cgccaccgat tgacccgagc gattcatgaa ggtgctcgga
     5461 cagagcagcc acagttcgcc actgcgcccg gcattgccga agaacttgcc ctgtggcgcc
     5521 agcgtcgtcc tcagctcgcg cgccagcgcc tcgaccagcc agaagccgac gttgtgacga
     5581 gtcgcctcgt actcggcgcc cggattgccg aggccgacga tcagtcgcag cttgctcatt
     5641 ccttaatagc ccgaacgctg gtgagaatga aaaaagccgc tgccggctcg cgccgcagcg
     5701 gctcttgccc gtcaagcgcc gattcaggcc ttgggagggg tggcagccgg ggtcgcagca
     5761 gccgaagccg cggcagccgt tgccgcgtcg gcggcctcgg cctcgtcggc agccgcatcg
     5821 ccacgaaccg tgaggatggt cgcaacaacc gggttgtcac cgccatgcag cacggccttg
     5881 acgcccttcg gcagcttgat ctcgtcgaca tgcagtgagt ggccggcgac cagatccttg
     5941 aggtcgactt cgaggaactc gggcagatcc gccggcagac acttgacctc gagttcggtc
     6001 atcacgtgcg agacgattcc accgccgagc ttcacgcccg gagcgatgtc gccgttaatg
     6061 aagtgcagcg gcaccgcgat gtgcagttcc ttcttcgcat cgacgcgctg gaagtcgacg
     6121 tgcagcacga gcttcttgta ggcgtgcgcc tgaacgtcgc gcaacaggac cgactccttt
     6181 ttgccatcga ggaccagcgt gatcaccgac gaatggaaag cttccttgcg cagcgagagc
     6241 agcagttcgt tgtggtccac ttccacgggc gtcggctgtg cagtgccgcc gtaaacgatg
     6301 ccggggacct tgccggcatg acgcaggcgg cggctcgcac ccgtgccctg cagcgtgcgc
     6361 gattgcgcgt tgagttcgaa ttgcatgtgt tgctcctaga gttgccgttc cgcgaccaga
     6421 acgacggttg aaaaaaacta ttcgagaaag agcgagctga ccgactcttc gttgcagata
     6481 cggcggatgg tctcggccag caggtcggcg accgacagga cgcgaatgcg ggggcaggct
     6541 gcggcctcgt cgcgcaacgg gatggtgtcg gtgacgacca gctcgtcgag cgccgaattg
     6601 ctgatccgct cgaccgccga gcccgagagc acggcgtgcg tgcagtacga catgaccttg
     6661 gccgcgccgt tggccttaag cgcctgcgcc gccttgcaga gcgtgccggc agtgtcgacg
     6721 atgtcgtcga tgatcacgca ggtgcggcct tcgacgtcgc cgatgatgtt catgacttcg
     6781 gagacgttgg ccttcggccg ccgcttgtcg atgattgcca ggtcgcactc gagtcgcttg
     6841 gccagcgagc gggcgcgaac gacgccgccg acgtcgggcg aaacgacgat cagctcgggg
     6901 taattctgcc gccagatgtc tcccagcagg attggcaggc cgtagatgtt gtccaccggc
     6961 atgtcgaaga aaccctgaat ctgctcggcg tgcaggtcca tcgtcagcac gcggtcgatg
     7021 ccggcggtcg tcagcatcga ggcgacgagc ttggcggcaa tcgggacgcg g
//
LOCUS       DATDAA010000153         2401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_185816,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000153 DATDAA010000000
VERSION     DATDAA010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185816"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VIS73_04995"
     CDS             <1..266
                     /locus_tag="VIS73_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEY5762542.1"
                     /translation="ADRRAHYVCVLAFVRRADDPQPLIAEGEWHGEIAAGPRGEGGFG
                     YDPYFWVPGAGCTVAQLDPVEKNRRSHRGQAMQLLAARLAAQA"
     gene            313..1518
                     /gene="hemW"
                     /locus_tag="VIS73_05000"
     CDS             313..1518
                     /gene="hemW"
                     /locus_tag="VIS73_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY5762543.1"
                     /translation="MMQTFIPVRRESDSQSPLPAAIPLSLYVHFPWCVRKCPYCDFNS
                     HEAPRHQDSDSGIPEDDYLTALVADLEAALPAIWGRRVGSVFIGGGTPSLLSEGGVDR
                     LLAALRARLPLSADAEITLEANPGTVEAARFRGYRAAGVNRVSLGVQSFDDTQLRALG
                     RIHGADEARRAVGLAAECFEQFNVDLMFGLPQQDVEALRRDLDEALRFAPPHLSAYQL
                     TIEPNTPFAAHPPPMPDDDEAADLADFVDQRLAQAGLMAYETSAYARAGSRCRHNLNY
                     WQFGDYLGIGPGAHSKLTLPGEIVREVRWKSPKDYLARRGEAAVRTRTRVAANDRAFE
                     FLMNALRLTEGFAIDDFAARTGQPWLSVAGEMGGAERDGLVTIAAGRVVPTLRGRRLL
                     NSLLQRFLP"
     gene            1665..2099
                     /gene="gspG"
                     /locus_tag="VIS73_05005"
     CDS             1665..2099
                     /gene="gspG"
                     /locus_tag="VIS73_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEY5762544.1"
                     /translation="MRFKTPEFRSRRSGGFTLLELLVVMVIIGLLAAYVGPRYFSQIG
                     KSEIKVARAQMDAFDKALEQYRLDVGRFPSTEQGLDALYTKPNSEPKWGGPYLKKAPP
                     PDPWGTPYQYKSPGESGEFDIISFGKDGRPGGNGEDADISFR"
     gene            2118..>2401
                     /locus_tag="VIS73_05010"
     CDS             2118..>2401
                     /locus_tag="VIS73_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5762545.1"
                     /translation="MRFLVKALNAGGAVVRVSIDAGDSTEARRLASGQGLRVLGLRPA
                     NLLGSLLPRTRSRFALDLFTRELISLLDTGLLLVEAIETLAEQEQHSSGGA"
BASE COUNT          365 a          831 c          788 g          417 t
ORIGIN      
        1 aggccgatcg ccgcgcgcac tacgtttgtg tgctggcctt tgtccgccgc gccgacgacc
       61 cgcagccgct gattgccgaa ggcgagtggc acggcgagat cgccgccggc ccgcggggtg
      121 agggcggctt cgggtacgac ccctacttct gggtgcccgg ggccgggtgc accgtcgcgc
      181 agctcgaccc cgtcgagaag aaccgccgct cgcatcgcgg ccaggcgatg cagttgctcg
      241 ccgcgcggct ggcggcgcaa gcctgaaggc aggcggaaag cgccgcatgg cgccaaatca
      301 ggttgttcgc acatgatgca aaccttcatc ccggttcgac gtgagagcga ttcgcaatcg
      361 ccgctgccgg ccgcgattcc gctctcgctc tacgtccatt tcccttggtg cgtgcgcaaa
      421 tgcccatact gcgatttcaa ttcgcacgag gcgccgcgtc accaggattc ggacagcggc
      481 attcccgagg acgactacct cacggcgctg gttgccgatc tcgaggctgc cttgccggcg
      541 atctggggtc gacgcgtcgg cagcgtcttc atcggcggtg gcacccccag tctgctttcg
      601 gaaggtggtg tcgatcggct gctggcggcg ctgcgcgccc gcttgccatt gtcggccgat
      661 gccgaaatca cgctcgaggc caatccgggc accgtcgaag ccgcgcgctt ccgcggctat
      721 cgcgccgccg gcgtgaaccg cgtttcgctc ggcgtgcaga gcttcgacga cacgcagctg
      781 cgtgcgctcg ggcgcatcca cggcgccgac gaggcgcgtc gcgccgtcgg actcgccgcc
      841 gagtgcttcg agcagttcaa tgtcgacctg atgttcgggc tgccgcagca ggacgtcgag
      901 gcgctgcggc gcgacctcga cgaggccctg cgctttgcgc cgccgcacct ctcggcctac
      961 cagctgacca tagagcccaa cacgccgttc gccgcccatc cgccgccgat gcccgacgac
     1021 gacgaggcgg ccgaccttgc cgatttcgtc gatcagcgcc tcgcccaggc gggcctcatg
     1081 gcctacgaga cttcggccta cgccagggcc ggttcgcgct gccggcacaa tctgaactac
     1141 tggcagttcg gcgattacct cggcattggc cccggcgctc acagcaaact gaccctgccc
     1201 ggggagatcg tccgcgaggt tcgctggaag tcgccgaagg actacctcgc gcgccgtggc
     1261 gaagcggcgg ttcgcacgcg tacccgcgtt gcggcgaatg accgcgcctt cgagttcctg
     1321 atgaacgcgc tgcgcctcac cgagggtttc gcgatcgacg atttcgccgc gcgtaccggc
     1381 cagccctggc tctcggtcgc cggcgagatg ggcggcgccg agcgcgacgg cttggtgacg
     1441 atagccgccg gccgggtcgt gccgacgctg cgcggacggc gcctgctcaa ttcgctgctg
     1501 caacgctttc tgccctgacc gcaaaaagcg gccgtcggct tcgatgatgg cccggacgcc
     1561 gttcgatgcg catttctgcc tgtacgcaag gcgatcaccg tgcagtcacg ccggttgcat
     1621 acgctagaat gccctgcgcc gacaaccttc aagaggttca tgacatgcgt ttcaagacac
     1681 ccgaattccg gtctcggcgc agcggcggtt ttacgctgct cgaattactg gtggtgatgg
     1741 tcatcatcgg cctgctagcc gcctacgtgg ggccgcgcta tttttcgcag atcggcaagt
     1801 ccgagatcaa ggtggcgcgc gcgcagatgg acgccttcga caaggcgcta gaacagtacc
     1861 ggctcgatgt cgggcgcttc ccgagcaccg aacagggcct cgatgcgctc tacaccaagc
     1921 cgaacagcga gcccaagtgg ggtggccctt acctgaagaa ggcgccgcca cccgatccct
     1981 ggggaacgcc ctaccagtac aagtcgccgg gcgaatcggg cgagttcgac atcatttcct
     2041 tcggcaagga cggacgcccc ggcggcaatg gcgaggacgc cgacatctcc ttccgctgat
     2101 cccgcgcgtc agaccgtatg cgctttctcg tcaaggccct caatgccggc ggtgccgtcg
     2161 tccgcgtcag catcgacgcc ggcgattcga ccgaggcgcg gcggctggcc agtggtcagg
     2221 gcctgcgcgt gcttggcctg cgcccggcca acctgctggg gagcctgctg ccgcgcacgc
     2281 ggtcgcgctt cgcgctcgat ctcttcacgc gcgaactgat ttcgctgctc gataccggtt
     2341 tgctgctggt cgaggcgatc gagacgctgg ccgagcagga gcagcattcg tcgggtggcg
     2401 c
//
LOCUS       DATDAA010000154         7511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_325777,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000154 DATDAA010000000
VERSION     DATDAA010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325777"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VIS73_05015"
     CDS             complement(<1..385)
                     /locus_tag="VIS73_05015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY5762546.1"
                     /translation="MNPRHTITLAAMLLSAASAAAHASNPAKDIDAFAQYMAIEHGFD
                     RAAVKRLLPSQPNPKILKAIQPSKTAERGSWTKYRENFINPGRIARGVAFWEENAEAL
                     EVAEARFGVPAEIIVAIIGVETNYGR"
     gene            complement(382..2340)
                     /locus_tag="VIS73_05020"
     CDS             complement(382..2340)
                     /locus_tag="VIS73_05020"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEY5762547.1"
                     /translation="MGKRKTEPPLDPRLAPWLIASAAVTLVPHFTHTSYWQIALIAAV
                     VAWRIWLWHRQAPLPSRWLITIITVAGVAGVALEYRRLFGQDSGVALLLLFMAMKLME
                     TRSPRDARVVVMLGYFLLLTHYFYQDGIPTGAWMIVALTVVTATLIRIETAALESGAL
                     LRKSAFMLAQSIPVMGILFLLFPRIDGPLWGLPQESRRNTTGLSEVMSPGSISALTQS
                     AEIAFRVQFRDEAPANSSLYWRGPVLSEFDGSTWRVQRLPPTLPTRPPVAEAGSATHH
                     YTLTLEPHQQRWLLALDMPLAAPRDAVMNSSYALLRRRAVQERIRYEAESATRHRAGI
                     AEANEILATATLLPRGSNPRTRQLVERWRAETGGGEALIAQALRHFRNEPFAYTLQPP
                     LLGEHPVDDFLFRTRRGFCEHYASAFAVMMRIAGMPARIVTGYQGGASNPVDGYWVVR
                     QADAHAWVEVWLRNRGWVRVDPTAAIAPSRIEAGVAAAIPQTEPLPGVVRGGIDWLRQ
                     ARYQWEALNNGWNQWVLGYDQARQTDLLGRLGLSTRWQSLIGTLVVAAAITVVVLALW
                     AMRRERQHDPAIRLWLAFTRHMARRGSAREPWEGPYDYARRLAERFPDLAEASTRFCA
                     AYARSRYGSATDDDLLTMTKTLKLIRSR"
     gene            complement(2321..3301)
                     /locus_tag="VIS73_05025"
     CDS             complement(2321..3301)
                     /locus_tag="VIS73_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY5762548.1"
                     /translation="MNTGDAVRSLPRRLGNWLFQGDRPVTSQLVLGQRQIFILPSAGG
                     ILYGLVLTVMYLGAVNYSLGLGHALVFLLAALGLTGMVHSFRNLAGLRLESVRGDPVH
                     VGETARFKIRLDNDRREPRPALTLAFKGEEAVTVDLAAGDAQVVGVPCRTTRRGWLVP
                     PRLTLFTRFPLGIFRAWSYPRLDFRILVYPAAIERPFPEPHPGDRPGTHGNDSGDDDF
                     AGFRPRQPGDPLRHVAWKNYARDPLDRPLQIKRFSGASTPDRVFAWDDLGDSATETKL
                     SILTGWALRASAAELGYTLRLPDRVIGPGSGVAHLHQCLKALALHGQTQD"
     gene            complement(3301..4248)
                     /locus_tag="VIS73_05030"
     CDS             complement(3301..4248)
                     /locus_tag="VIS73_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5762549.1"
                     /translation="MGATAPPTTPLSVTTVRAVITAAAEIVLGKPRQIRLAIACLLAR
                     GHLLIEDLPGVGKTTLAQTLAHLLGLQYQRVQFTSDLLPADILGVSVFDRDHAAFRFL
                     PGPVFAQLVLADEINRATPKTQSALLEAMEERQVTVEGETRPLPDPFFVIATQNPSHQ
                     IGTFPLPESQLDRFLMRLSLGYPDRGAELALLAGGDQREALARLMPIIQPSDLPPLQA
                     AVRRIKVAPALLNYVQALLQRTRDDSRFAHGLSPRAGIGIVTAARSWAFLEGRDHVAP
                     EDVQAVFPEVACHRLRLAESGGHALPEDAQAIVRATPLP"
     gene            4376..5302
                     /locus_tag="VIS73_05035"
     CDS             4376..5302
                     /locus_tag="VIS73_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY5762550.1"
                     /translation="MTTTAFITHSECQLHDMGSFHPECPARLSAINDHLIAQGIDGYF
                     PNHEAPLATLEHLLRAHPPEHLERLKRNVPKQGVQHLDPDTAMCPHTWQAALRAAGAG
                     ILAVDLVMGKKVENAFCSVRPPGHHAERTNAMGFCFVNNIAIAALHALVHHGLSRVAI
                     IDFDVHHGNGTEDIFAGDDRVLMASIFQHPFYPYSGADHPAANMINVPLAGGAGSEDF
                     RGAVTDVWLPALERFKPEMIFISAGFDAHYEDDMGGLKLFEKDYAWVTTQIKAVADKY
                     AAGRIVSMLEGGYVLSALARSVAVHLRALAEI"
     gene            5382..6260
                     /gene="dapA"
                     /locus_tag="VIS73_05040"
     CDS             5382..6260
                     /gene="dapA"
                     /locus_tag="VIS73_05040"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY5762551.1"
                     /translation="MITGSIVAIVTPMHEDGSLDFEAFRRLVDHHIAEGTDAIVVAGT
                     TGESPTVDVDEHCELIKVAVECAARRVPIIAGTGANSTAEAIELSRFARRVGANACLS
                     VVPYYNKPTQEGLYRHFRAIAEAVDTPVILYNVPGRTVADLANDTALRLAEIDNVVGI
                     KDATGNLERGADLLTRAPEGFAVYSGDDASAIALMFLGGHGNISVTANVAPRLMHEMC
                     AAAMAGDVVRARELHFRLLPLHRNLFVEANPIPVKWACTKLGMIPPGIRLPLTALSAG
                     GQEAVTRALQQAGLLV"
     gene            6257..7417
                     /gene="bamC"
                     /locus_tag="VIS73_05045"
     CDS             6257..7417
                     /gene="bamC"
                     /locus_tag="VIS73_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEY5762552.1"
                     /translation="MRRAIGNVRAGQRLAGRVGITLLVVALAGCAMFDSKVDYKSVKR
                     DKPLEVPPELTVPQTGDRFAIPGAAASTTYSDYERSRAGSRAAPSAGIVLQPESSPTV
                     RIERAGTQRWLVVQAPPEQVWAQLKEFWQSQQIPLELELPAVGVMETKWVEERAKFSA
                     GAIRDMLSRVMDVFYSNPERNKFRTRIEKGIEPGTTEVYISHRQMFEMFVDEGQSSTR
                     WQPQPAQPGIEAEMLRRFVVFTGVGEKAARELIVAQSAPTPDRSRLVEREGRAALEMD
                     ERFDRAWRRTGLSLDRIGFTVEDRNRAEGVFFVRYLDSDAAALRESKKGFFSWLAFWQ
                     DDEEEKIQTEFRVVVMGENDRSYVTVRPRKDGDPEDLPTGRKILTLLNEQLR"
BASE COUNT         1274 a         2369 c         2626 g         1242 t
ORIGIN      
        1 tgcggccgta gttggtctcg acgccgatga tcgcgacgat gatttctgcc ggcacgccga
       61 agcgtgcctc ggcaacttcc agcgcctcgg cgttctcttc ccagaaggcg acgccgcggg
      121 cgatgcgccc cggattgatg aagttctcgc ggtacttggt ccacgagcca cgctcggcgg
      181 tcttcgaagg ctggattgct ttgaggatct tcggattcgg ctggctcggc agcagccgtt
      241 tgacggccgc ccggtcgaat ccgtgttcga tcgccatgta ctgggcgaag gcgtcgatgt
      301 ctttcgcggg gttgcttgcg tgcgcggcgg ccgatgctgc gctgagcagc atggcggcga
      361 gtgtgatggt gtggcgtggg ttcatcggct gcggatcagt ttcagggtct tggtcatggt
      421 caggagatcg tcgtcggtcg cgctgccgta gcggctgcgc gcataggcgg cgcagaaacg
      481 tgtgctcgcc tcggcgaggt cgggaaagcg ctcggccagc cgtcgggcgt aatcatacgg
      541 gccctcccag ggttcgcggg cgctgccgcg gcgtgccatg tgtcgcgtga aggcgagcca
      601 gagccggatc gccggatcgt gttggcgttc gcgccgcatt gcccaaagcg cgagaacgac
      661 gaccgtgatt gccgctgcga cgaccaaagt gccgattagc gactgccagc gggtggagag
      721 gccgaggcga ccgagcaggt cggtctggcg tgcctgatcg tagccgagca cccactggtt
      781 ccagccgttg ttgagcgcct cccactggta acgcgcctgc cgcaaccagt cgatgccgcc
      841 acggacgacg ccgggcagcg gctcggtctg ggggatcgcc gcggcgacgc ccgcctcgat
      901 gcggcttggc gcgattgccg ccgtcgggtc gacgcggacc cagccgcgat tacgcagcca
      961 gacttcgacc caggcgtgcg catcggcctg gcgaaccacc caatagccgt cgaccggatt
     1021 ggacgcgccg ccctgatagc cggtgacgat gcgcgccggc atgccggcaa tgcgcatcat
     1081 cacggcgaag gccgaggcgt agtgctcgca gaagccgcgc cgggtgcgga agaggaagtc
     1141 atcaaccggg tgctcgccga gcagcggcgg ctgcaacgtg taggcgaagg gctcgttgcg
     1201 gaagtgccgc agcgcctgcg caatcagcgc ttcgccgccg ccggtttcgg cacgccagcg
     1261 ctcgacgagc tggcgcgttc gcgggttcga tccacgcggc agcagcgtcg ccgtggcgag
     1321 aatctcgttg gcttcggcga tgccggcgcg atggcgggtg gccgattcgg cctcgtagcg
     1381 gatgcgctcc tggaccgccc gccggcggag cagcgcatag ctgctgttca tcaccgcatc
     1441 gcgcggcgcc gccagcggca tgtcgagcgc cagcagccat cgctgctggt gcggttcgag
     1501 tgtcagcgtg tagtggtgcg tcgcgctgcc agcctcggcg accggcggcc gggttggcag
     1561 cgtcggcggt aagcgctgga cccgccaggt gctgccgtcg aactcgctga ggaccggacc
     1621 ccgccagtag agggagctgt tggccggcgc ttcgtcgcgg aactgcacgc gaaacgcgat
     1681 ttcggccgat tgcgtcagtg cgctgatcga tcccggcgac atcacttcgg acaggccggt
     1741 ggtgttgcgc cggctttcct gcggcaagcc ccagagcggt ccgtcgatgc gtggaaagag
     1801 caggaagagg atgcccatca ccggaatcga ctgcgccagc atgaaagccg acttgcgcag
     1861 gagcgcaccc gattcgagcg cggcggtttc gatgcggatc agcgtcgccg tgacgacggt
     1921 cagcgcgacg atcatccagg cgccagtggg aatgccgtct tggtagaagt agtgtgtcag
     1981 cagcaggaag tagccgagca tgacgacgac ccgcgcgtcg cgcggactgc gcgtctccat
     2041 cagcttcatc gccatgaaca gcagcagcag cgcgacaccc gaatcctggc cgaataggcg
     2101 ccggtattcg agggcgacgc cggcgacgcc ggcaaccgtg atgatcgtga tcaaccagcg
     2161 tgacggcagc ggcgcctgcc tgtgccacag ccagattcgc caggcgacga cggcggcgat
     2221 cagcgcgatc tgccagtaag acgtatgcgt gaaatgcggc accagcgtga ccgctgccga
     2281 agcgatcagc catggcgcca gacgcggatc gagcggcggc tcagtcttgc gtttgcccat
     2341 ggagcgcgag cgccttcagg cattgatgca gatgtgcgac gcccgaaccg gggccgatca
     2401 cccggtcggg caggcgcagc gtgtagccga gttcggcagc gctggcgcga agcgcccagc
     2461 cggtgagaat cgacagcttc gtctcggtcg ccgagtcgcc gagatcatcc caggcgaaga
     2521 cacgatcagg cgtgctggcg cccgagaagc gcttgatctg cagcggtcgg tcgagcgggt
     2581 cgcgtgcgta gttcttccag gcgacgtggc gcagcggatc gccgggctgg cgcgggcgaa
     2641 agccggcaaa gtcgtcgtcg ccgctgtcgt tgccgtgcgt acccggacgg tcgcccgggt
     2701 gcggttcggg aaacggccgc tcgatggcag cagggtagac gaggatgcgg aaatcgaggc
     2761 gtggatagct ccaggcgcgg aagatgccca gcggaaagcg cgtgaacagc gtcaggcgcg
     2821 gcggcaccag ccaaccgcgg cgcgttgtgc ggcagggcac gccgaccacc tgcgcgtcgc
     2881 ccgccgcgag gtcgacggtg accgcttcct cgcccttaaa ggccagcgtc agcgccgggc
     2941 gcggctcgcg ccggtcgttg tcgagacgga tcttgaaacg cgcagtctcg ccgacatgaa
     3001 cggggtcgcc gcggacgctc tcgaggcgca ggccggcgag gttgcgaaag ctgtggacca
     3061 tgccagtgag cccgagcgcg gcgagcagga agaccagcgc atggccgagg ccgaggctgt
     3121 agttgaccgc gccgaggtac atgacggtca gcaccaagcc gtagaggatg ccgccggcgc
     3181 tgggcaggat gaatatctgc cgctggccga gcacgagctg gctggtaacc ggccggtcgc
     3241 cctgaaacag ccagttgccg agcctcctcg ggaggctgcg gacggcgtcg cccgtgttca
     3301 tcagggcaag ggagtggcgc gaacgatcgc ctgggcgtcc tcgggcaggg cgtggccgcc
     3361 cgattcggcc aaccgcaggc ggtggcaggc aacttcggga aagaccgcct ggacgtcctc
     3421 gggtgcgaca tggtcgcggc cctcgaggaa ggcccagcta cgcgccgcgg tgacgatgcc
     3481 gatgccggcg cgcggcgaca gcccgtgggc aaagcggctg tcgtcgcggg tccgctgcag
     3541 cagcgcctgt acgtagttca gcaatgccgg tgctaccttg atgcggcgta ccgccgcctg
     3601 cagtggcggc agatcactcg gttggatgat cggcatcagg cgcgccagcg cttcgcgctg
     3661 gtcgccgccg gcgagcagcg ccagctcggc gccacgatcg ggatagccga gcgacagccg
     3721 catcaggaaa cggtcgagct gcgactcggg cagcggaaag gtgccgattt ggtgcgaagg
     3781 gttctgcgtg gcgatgacga aaaaggggtc ggggagcggc cgggtttctc cttcgacggt
     3841 gacctgccgc tcctccatcg cctcgagcag tgcgctctgt gtcttcggtg tcgcgcggtt
     3901 gatttcgtcg gccagcacca gctgggcaaa caccgggccg ggcaggaagc ggaaggccgc
     3961 gtgatcgcgg tcgaagaccg aaacgccgag gatgtcggcc ggcagcaggt cgctggtgaa
     4021 ctggacgcgc tggtactgga ggccgagcag atgcgccagc gtctgcgcca gcgtcgtctt
     4081 gccgacgccg ggcagatctt cgatcagcag gtggccgcgc gccagcaggc aggcgatcgc
     4141 cagccggatc tgccgcggct tgccgaggac gatttcggcg gcggcggtga tgaccgcgcg
     4201 gaccgtggtg accgacagcg gggttgtcgg gggggctgtc gcgcccaccg cggtttccgg
     4261 attacgcatg gcttgaggca atcggtctaa ggccagataa tggtgagaga ccgcagcatt
     4321 gaggctgcgg ttcattacat tacgccacag tttggcgccc ttggggacgg agaacatgac
     4381 gaccacggcg ttcatcacgc acagcgagtg tcagcttcac gacatgggtt cgtttcaccc
     4441 ggagtgtccg gcgcgcctct ccgcgatcaa cgaccacctc atcgcgcaag gcatcgacgg
     4501 ctacttcccg aatcacgagg cgccgctggc aacgctcgag catctgttgc gcgcgcatcc
     4561 gcccgagcat ctcgagcgcc taaagcgcaa tgtccccaaa cagggtgtgc aacacctcga
     4621 ccccgatacc gcgatgtgtc cgcatacctg gcaggcggcc ttgcgtgccg ccggtgccgg
     4681 cattcttgcc gtcgacctgg tgatgggcaa aaaggtcgag aacgcgttct gctcggttcg
     4741 gccaccgggg catcatgccg agcgaaccaa cgcgatgggc ttctgcttcg ttaacaacat
     4801 cgcgatcgcg gcgctgcacg cgctcgtgca ccacggcctg tcgcgtgtgg cgatcatcga
     4861 ttttgacgtg caccacggca acggcaccga ggacatcttt gccggcgacg accgggtgct
     4921 gatggcgagc atcttccagc accccttcta cccctacagc ggggccgacc atccagcggc
     4981 aaacatgatc aacgttcccc tcgccggcgg tgcgggcagc gaggacttcc ggggcgcggt
     5041 gaccgatgta tggctgccgg cactcgagcg cttcaagccc gaaatgatct ttatctcggc
     5101 cggcttcgat gcccattacg aagacgacat gggcggcctc aagctgttcg agaaggatta
     5161 cgcgtgggtg accacgcaga tcaaggcggt ggccgacaag tacgcggctg gccgcatcgt
     5221 ctccatgctc gagggcggct acgttctgtc ggcgctggca cgcagcgtcg ccgtgcatct
     5281 gcgggcgctg gccgagatct gagcgacggc cgattcccgg gcggcggcac ctgccgccat
     5341 cgggtagaat ccgcccttcc attttctggc ggaagcattc catgatcacc ggttcgatcg
     5401 ttgccatcgt cacgccgatg cacgaagacg gcagcctcga tttcgaggcc tttcgacgcc
     5461 tcgtcgacca ccacatagcc gagggcaccg acgctatcgt cgttgccgga accaccggcg
     5521 agtcgccgac cgtcgatgtt gacgagcatt gcgaactgat caaggtcgcc gttgaatgcg
     5581 ccgcccggcg cgtgccgatc atcgctggca ccggagccaa ttcgactgcc gaggcgatcg
     5641 agctgagccg attcgcccgc cgggtcggcg ccaatgcctg cctttcggtc gtgccctact
     5701 acaacaaacc gacgcaggaa ggcctctacc ggcatttccg cgcgatcgcc gaagcggtcg
     5761 atacgccggt gattctctac aacgttcccg gccgcaccgt cgccgatctc gccaacgata
     5821 cggcgttgcg cctggccgag atcgacaacg tcgtcggcat caaggatgcg accggcaacc
     5881 tcgagcgcgg cgccgatctg ctgacgcgcg caccggaggg ctttgccgtc tatagcggcg
     5941 acgatgcctc agcgattgcc ctgatgttcc tcggtggaca tggcaatatt tcggtgactg
     6001 ccaacgtcgc gccacgcctg atgcacgaga tgtgcgcggc ggcgatggca ggcgatgtgg
     6061 tgcgcgcacg ggaattgcat ttccgcctgt tgcccctgca tcgcaacctg tttgtcgaag
     6121 ccaatccgat tccggtcaag tgggcgtgca ccaagctcgg gatgattccc ccgggcatcc
     6181 gcctgccgct gaccgcgctg agtgcgggcg ggcaggaagc ggttacccgc gcgctgcagc
     6241 aagccggtct gctggtatga gacgcgcaat cggaaacgta cgggccgggc aacggctcgc
     6301 ggggcgcgtc ggcatcacgc tgctggtcgt tgcgctggcc ggctgcgcga tgttcgattc
     6361 aaaggtcgac tacaagtcgg tcaagcgcga caagccgctt gaggtgccgc cggaactgac
     6421 ggtgccgcag accggagatc gttttgcgat tccgggcgcg gcggcctcga caacctactc
     6481 cgattacgag cgcagtcgcg ccggtagccg cgcggcacca tcggccggta tcgttctgca
     6541 gccggaaagc agtccgacgg ttcgcatcga acgcgccggt acgcagcgct ggttggtcgt
     6601 gcaggcgccg cccgagcagg tctgggccca actcaaggag ttctggcagt cgcagcagat
     6661 tccactggaa ctggagttgc cggccgttgg cgtgatggaa accaagtggg tagaggaacg
     6721 ggccaagttc tcagccggcg ctatccgcga catgctctcg cgcgtgatgg atgtcttcta
     6781 ttcgaaccct gaacgaaaca aattccgcac acgaatcgaa aagggaatcg agcccggtac
     6841 gaccgaggtc tacatcagcc atcggcagat gttcgagatg tttgtcgacg aaggtcagtc
     6901 gtctacgcgc tggcagccgc agccggctca gccgggaatc gaagccgaga tgctgcgtcg
     6961 tttcgtcgtg tttacaggtg tcggcgaaaa ggcggcgcgc gagctgatcg tcgcgcagtc
     7021 ggcgccaaca ccggaccgca gtcgactcgt cgagcgcgag ggccgtgcgg ccctggaaat
     7081 ggacgagcgc ttcgatcgcg cctggcggcg gaccgggctg tcgctcgacc gcatcggttt
     7141 caccgtcgaa gaccgcaatc gcgccgaggg ggtgtttttt gtccgctacc tcgacagcga
     7201 tgcggcggcc ctgcgcgagt cgaagaaggg gttcttctcg tggctggcgt tctggcaaga
     7261 cgacgaggaa gagaagatac agactgagtt tcgcgtcgtc gtgatgggcg agaacgaccg
     7321 tagctacgtt acggtgcgtc cgcgcaagga tggcgacccc gaggacctgc ctaccggccg
     7381 caagatcctg acgctgctca acgaacagct gcgctgagga tgcgctttgc gctcctcggc
     7441 agcggcagcc gtggcaacgc cacggtcgtc gaggctggcg gcggtgcgtc gacgacgcgt
     7501 ctgctgctcg a
//
LOCUS       DATDAA010000155         3858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_396027,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000155 DATDAA010000000
VERSION     DATDAA010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396027"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /gene="hpnC"
                     /locus_tag="VIS73_05050"
     CDS             complement(<1..574)
                     /gene="hpnC"
                     /locus_tag="VIS73_05050"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEY5762553.1"
                     /translation="MPVDHYENFPVASFLLPAAIRRPVETIYCFARSADDVADEGDAT
                     AAERLAALDGYRALLDRIDAGEDPADPRFPELAATIAAHALPTQLFRDLIDAFAQDVT
                     TLRYADDASLRDYCRRSANPVGRLLLRLVGVDDASALACSDRICTALQLINFLQDIAI
                     DWQKGRVYLPQTALAKFAIKEEQIAAGRWDA"
     gene            737..821
                     /locus_tag="VIS73_05055"
     tRNA            737..821
                     /locus_tag="VIS73_05055"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:771..773,aa:Leu,seq:tag)
     gene            906..2216
                     /gene="tig"
                     /locus_tag="VIS73_05060"
     CDS             906..2216
                     /gene="tig"
                     /locus_tag="VIS73_05060"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY5762554.1"
                     /translation="MSSTAEATASNKLERRVDLSVPIADLEQNVDNRLKKLGKNVKMP
                     GFRPGKVPFSIIKQQYGDQARSEALTEVLDKAFGEAIQAQSLRVAGYPQIEPKQTESK
                     THVEFSAIFEVYPEIVVGDLTGETVEKPVFEVTPAEVDRTIDILRKQRVEYIDADRAA
                     AKGDRVVIDFTGRKDGEVFQGGQANDYPFVLGEGAMLPEFEAAVEGLAKGEKKNFDLT
                     FPADYFSKDLAGQGVQFEVTVKSVQAAKLPEIDDAFAVSLGIAGGDVAAMRAEIEANL
                     KREVKRRIKGRIKDQVMDALLKVTPIDVPSALVDAEVHRLMEAARQDMAQRGMKAKDL
                     PMQPQWFTEQAKRRVSLGLVLAELVKAEKLHADAEKVRAMVDDFAQTYENPDEVVSWY
                     YAQPQRLAEVEALVLEDNVVAWVLARAKATDKTVAFDDLMGRNA"
     gene            2261..2920
                     /gene="clpP"
                     /locus_tag="VIS73_05065"
     CDS             2261..2920
                     /gene="clpP"
                     /locus_tag="VIS73_05065"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEY5762555.1"
                     /translation="MTRIQQQDWQPQASALDTQALGMVPMVIEQSGRGERAYDIYSRL
                     LRERVVFLVGPVNDATANLVVAQLLFLEADNPDKDVYFYINSPGGSVTAGLAIYDTMQ
                     FIKPDVSTLCIGQAASMGAFLLAAGAKGKRFALPNSRVMIHQPMGGFQGQASDIAIHA
                     KEILSLRQRLNEIMATHTGQPIERIERDTDRDNFLSASESAEYGLIDKVLTSRAAMAT
                     E"
     gene            2948..>3858
                     /gene="clpX"
                     /locus_tag="VIS73_05070"
     CDS             2948..>3858
                     /gene="clpX"
                     /locus_tag="VIS73_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY5762556.1"
                     /translation="MAEKKGTEKLLYCSFCGKSQHEVKKLIAGPSVFICDECIELCND
                     IIRDEIAGENGTAGGGDALPSPHEIAGILDQYVIGQARAKRILSVAVYNHYKRLRHSD
                     KSKDEVELAKSNILLIGPTGSGKTLLAQTLARLLNVPFVMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVEKAQQGIVYIDEIDKISRKSDNPSITRDVSGEGVQPALLKLIEGT
                     VASVPPQGGRKHPNQDFVQVDTTNILFVCGGAFDGLERVIRERSERGGIGFGAEVRSK
                     NDKQALGEVIAGVEPEDLIKFGLIPEFV"
BASE COUNT          763 a         1155 c         1298 g          642 t
ORIGIN      
        1 tggcgtccca acgcccggcg gcaatctgct cttccttgat ggcgaacttc gccagcgccg
       61 tctgcggcag gtacacgcga cccttctgcc agtcgatggc gatgtcctgg aggaagttga
      121 tcagctgcag cgccgtgcag attcggtccg agcaggccag cgcggatgca tcgtcgacgc
      181 cgaccagacg cagcaacagg cggccgaccg ggttggccga gcgccggcag tagtcgcgca
      241 ggctggcgtc gtcggcgtag cgaagcgtcg taacgtcttg cgcaaaggca tcgatcaggt
      301 cgcggaagag ttgtgtgggc agcgcatgag cggcgatggt ggcggcgagc tcgggaaagc
      361 gcggatcggc cggatcttcg ccggcgtcaa tgcggtcaag gagggcacga tagccgtcca
      421 gtgcggccag ccgttccgcg gccgtcgcgt cgccttcgtc ggccacgtcg tcggcgctgc
      481 gggcgaagca atagatcgtt tcgacgggcc ggcgaatcgc tgcgggaagc agaaacgacg
      541 cgaccgggaa gttttcgtag tgatcgaccg gcatggtgtg gtgggtccgg gtgccgccgg
      601 ggagcggcct ggtggcggcg ccgcgcagta taggcgctga aattgactgc cgtcagcgcc
      661 cggcgttggc tgtcggtgca ggcgacggtg caggcgactg cagcctcggg taaaatcgcc
      721 ggcttgtcgc cgggcggcga gagtggcgaa attggtagac gcactggatt taggttccag
      781 cgccgcaagg tgtgtgggtt cgagtccctc ctctcgcacc agtccaacgg ctggcggatt
      841 ttccgcaccg tggctgaccc ggttgttttt acaggcgaat ttcgccggtt ttcctgggaa
      901 tcctcatgtc aagcaccgca gaagccaccg cgtccaacaa gctggagcgt cgcgtcgatc
      961 tatcggttcc gattgccgat ctcgagcaga acgtcgacaa ccggctgaag aaactcggca
     1021 agaacgtcaa gatgcccggc ttccgccccg gcaaggtacc gttttcgatc atcaagcagc
     1081 aatatggcga tcaggctcgt tcggaggcgc tgaccgaggt cctcgacaag gccttcggcg
     1141 aagcgatcca ggcccagagc ctgcgcgttg ccggctatcc gcagatcgag ccgaagcaga
     1201 ccgagagcaa gactcacgtc gaatttagtg ccatctttga ggtgtatccg gaaatcgtcg
     1261 tcggcgacct cacgggcgag accgtcgaga agcccgtctt tgaggtgacg ccggccgaag
     1321 ttgaccgcac catcgacatc ctgcgcaagc agcgcgtgga atacatcgac gccgatcgcg
     1381 ccgccgccaa gggcgaccgc gtggtcatcg acttcaccgg gcgcaaggac ggcgaggtgt
     1441 ttcaaggcgg gcaggcgaac gactatccgt tcgtgctcgg cgagggcgcg atgctgccgg
     1501 agttcgaggc ggcggtggaa ggcctcgcca agggcgagaa gaagaatttc gacctgactt
     1561 ttccagccga ctatttttcc aaggatctcg ccggccaggg cgtccagttc gaggtgacgg
     1621 tgaagagcgt tcaggcggcc aaactgcccg agatcgacga tgccttcgcg gtgtcgctcg
     1681 gcatcgccgg tggcgacgtg gcggcgatgc gtgccgaaat cgaagccaac ctcaagcgcg
     1741 aggtgaagcg gcggatcaag ggccggatca aggatcaggt catggacgca ctgctcaagg
     1801 tcaccccgat cgacgtaccg tcggcactgg tcgacgccga agtgcatcgg ctgatggaag
     1861 ccgcacgcca ggacatggcg cagcgcggca tgaaggccaa ggatctgccg atgcaaccgc
     1921 agtggttcac cgagcaggca aagcgccgcg tgtcgctggg cctggtgctg gccgaactgg
     1981 tcaaggccga gaagcttcac gccgatgccg agaaggtacg ggcgatggtt gacgatttcg
     2041 cgcagaccta cgagaacccc gacgaagtcg tcagctggta ctacgcgcag ccgcagcggc
     2101 tggccgaagt cgaggcgctg gtccttgagg acaatgtggt cgcttgggtg ctggcacggg
     2161 ccaaggccac cgacaagact gtcgcctttg acgacctgat ggggcgtaac gcctgatttg
     2221 ggctagagtt gcaattgagc agcacacagc gggaagacga atgacacgga tacagcaaca
     2281 ggattggcag ccacaggcat cggccctcga cacgcaggcg ctgggcatgg tgccgatggt
     2341 gatcgaacag agcggacgag gcgaacgggc ctacgacatc tactcgcggc tgctgcgcga
     2401 gcgggtggtt tttctggtcg gcccggtcaa cgacgcaacc gccaacctgg tcgttgccca
     2461 gctgctgttc ctcgaggccg acaaccccga caaggatgtg tacttctaca tcaactcgcc
     2521 gggaggctcg gtcacggctg gcttggcgat ctacgacacg atgcagttca tcaagcccga
     2581 cgtgtcgacg ctgtgcatcg gtcaggccgc gtcgatgggc gcctttctgc tggcagccgg
     2641 cgccaagggc aagcgtttcg cgcttcccaa ctcgcgggtg atgattcatc agccgatggg
     2701 tggctttcag gggcaggcct cggacatcgc gatccatgcc aaggaaatcc tctccctgcg
     2761 ccagcgcctc aacgaaatca tggcaaccca cacggggcag ccgatcgagc ggatcgagcg
     2821 cgataccgac cgtgacaact tcctctcggc gagcgaatcg gccgaatacg gattgatcga
     2881 taaggtgctg accagtcgcg cggcgatggc gaccgagtga gtaccggcaa ggcagcggag
     2941 aacggcgatg gctgagaaaa agggaaccga gaaactgctt tactgctcgt tttgcggcaa
     3001 gagccagcac gaggtcaaga agctgattgc cggtccgtcg gtgttcatct gcgacgaatg
     3061 catcgagctg tgcaacgaca tcatccgtga cgaaatcgcc ggcgagaacg gcaccgccgg
     3121 cggcggcgat gcgctgccgt cgccgcacga gattgccggc atcctcgacc agtacgtcat
     3181 cggccaggcc cgtgccaagc ggatcctatc ggtcgccgtc tacaaccatt acaagcgcct
     3241 gcgccacagc gacaagtcga aggacgaggt cgagctggcg aagagcaaca tcctgctgat
     3301 cggcccgacc ggctcgggca agaccctgct ggcacagacg ctggcgcggc tgctcaacgt
     3361 tcctttcgtc atggccgacg cgacgacgct gaccgaagcc ggctatgtcg gcgaggacgt
     3421 cgagaacatc atccagaagc tcctgcagaa gtgcgactac gacgtcgaga aggcgcagca
     3481 gggcatcgtc tacattgacg agatcgacaa gatctcgcgc aagagcgaca acccctcgat
     3541 cacccgcgac gtctcgggcg agggcgtcca gccggcgctg ctcaagctga tcgagggtac
     3601 ggtcgcctcg gtgccgccgc agggcggccg caagcacccc aatcaggact tcgtgcaggt
     3661 cgatacgacc aatatcctgt tcgtctgcgg cggggccttc gatggtctcg agcgggtcat
     3721 ccgcgagcgc tccgaacgcg gcggcatcgg ctttggagcc gaggttcgca gcaagaacga
     3781 caagcaggcg ctgggcgagg tgatcgcagg ggtcgagccc gaggatctga tcaagttcgg
     3841 actgatcccc gagttcgt
//
LOCUS       DATDAA010000156         6687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_11195,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000156 DATDAA010000000
VERSION     DATDAA010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6687
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11195"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VIS73_05075"
     CDS             complement(<1..214)
                     /locus_tag="VIS73_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762557.1"
                     /translation="MITQNPAATDAKYIEREHQVQLRLIQTVEAGLAENRDVAESIEA
                     LADYSRAHFLSEELLMRQYAYGDYDDH"
     gene            complement(286..1626)
                     /gene="mnmE"
                     /locus_tag="VIS73_05080"
     CDS             complement(286..1626)
                     /gene="mnmE"
                     /locus_tag="VIS73_05080"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEY5762558.1"
                     /translation="MARTDPANRDTIAAVATPPGRGGVGIVRISGAGLDRFAAALIGA
                     QLAPRLATRCHFRDAAGETIDDGLAIAFTAPNSFTGEDVLELHGHGSPVAIAQLLARC
                     HQLGARPARPGEFSERAFLNGKLDLVQAEAIADLIDASTAAAARSAIRSLSGAFSEAI
                     AAVAHELVRLRMLTEATLDFSDEEIDALSPDAAQRAVEQIEVNLTALRRDADRGKLLR
                     DGLSVVLIGRPNVGKSSLMNHLAGDEIAIVSEYEGTTRDSLKRTVQLRGIPVHVVDTA
                     GLRDTDDPVEQIGIARTWREISKADVALVIADARTGVQPEDRALIDRLPPGLQVLTVL
                     NKSDLAKTAHSTEAAALAVSARTGEGLPALEERLLAIAHWHPGEATFIARERHLDALR
                     RCADHLSDARGSASQLELFAEELRLAHRALQEITGEYSTEDLLGEIFSGFCIGK"
     gene            complement(1601..3262)
                     /gene="yidC"
                     /locus_tag="VIS73_05085"
     CDS             complement(1601..3262)
                     /gene="yidC"
                     /locus_tag="VIS73_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY5762559.1"
                     /translation="MDTRRLIILAVFFLSIYMLWTEWERYNNPAPPPAATTAAPAVAT
                     GAPTVSDALSTPNPTLAAPGSQQVPAASATAAAETPKPIRVRTDKLVADISPIGGEIL
                     RLELLDHRATESPDRNLLLVDPDHRYAIQSGLIGDGLPTHRANFRIVSNETELGDRQS
                     LVVDLEADGGPGVVVRKRYEFRRGDYRVLITQQIDNRGTTPLTTHAYYQFQRDGRSPP
                     GESSMTMTYTGPAIYTEVEKFKKISFDDIAGGGAKFPTITQDGWIAMVQHYFVAAWLP
                     PATGAREFFMRKLEGDLYTAGVIVPVAEIAPGSSAEATMTLYAGPQEQATLEKLAPGL
                     DLVVDYGWLTVIAAPIFWMLDFIHGFVGNWGWAIVILTILIKLAFFPLSAASYRSMAR
                     MRQMTPRMMQIKERYGDDRQRMNQEMMELYRKEKINPLGGCLPILIQIPVFIALYWVL
                     LGAVEMRGAPWILWIHDLSRADPYYVLPVVMVVSMIIQMRLNPTPPDPIQAKIMMAMP
                     FVFGVMFFFFPAGLVLYWVVNNILSIGQQWQITRMIEGGKDRSGK"
     gene            complement(3288..3497)
                     /gene="yidD"
                     /locus_tag="VIS73_05090"
     CDS             complement(3288..3497)
                     /gene="yidD"
                     /locus_tag="VIS73_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY5762560.1"
                     /translation="MKRLLIVLVKGYRLMISPFLPPSCRHAPSCSEYAVEALTRHGAT
                     RGSWLTLKRISRCHPWHDGGYDPVP"
     gene            complement(3494..3880)
                     /locus_tag="VIS73_05095"
     CDS             complement(3494..3880)
                     /locus_tag="VIS73_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY5762561.1"
                     /translation="MAGSKRLPRAARLADPEHFRQAFASRRRLKGRWMNIHLRQRGDG
                     DDEKARIGLVIGKRFCRLATRRNFLKRLIRERFRAVRTDLGSVDLVVRLVRNLPQQLE
                     AETRESLRLDLDSLLAKLNQTLRRPT"
     gene            complement(3892..4026)
                     /gene="rpmH"
                     /locus_tag="VIS73_05100"
     CDS             complement(3892..4026)
                     /gene="rpmH"
                     /locus_tag="VIS73_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY5762562.1"
                     /translation="MKRTYQPSVIRRKRTHGFLVRSRTRGGRSVLAARRAKGRRRLSV
                     "
     gene            4330..5724
                     /gene="dnaA"
                     /locus_tag="VIS73_05105"
     CDS             4330..5724
                     /gene="dnaA"
                     /locus_tag="VIS73_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY5762563.1"
                     /translation="MEVFWNTCLSQLAEEMPAQQFSTWIRPLTMEPVTDGNGKRLRIV
                     APNGFILKWVRERFLARIASMAESHFGEPVVIDIVAGGAVASGERKREAAVALAEAPV
                     AAARQAAVPMINGVAAANGTEPDRGRLINGFTFDNLVVGKANDLARAAGMQVAEHPGG
                     AYNPLFVYGGAGLGKTHLIHAIGNFIADQSPSKSIRYIHAEDYYNDVVRAYQQKAFDA
                     FKRSYRSVDVLLLDDVQFFNGKNRSQEEFFFLFNSMIEARKQIVITCDTYPKDINGLE
                     DRLITRFDWGLTVQIEPPELEMRVAIIKKKAEGNGIALPDDVAFFIAKHLRSNVRELE
                     GALNRVLAYARFHGQAISVELSKDALKDVVGAFNRQITIDNIQKTVADYFNIRVGDLY
                     SKKRARIIVRPRQVAMWLCKEVTPHSYPVIGEAFGGRDHTTVIHAVRTIEAHRAKDGE
                     LNHALHVLLQVLKG"
     gene            5951..>6687
                     /gene="dnaN"
                     /locus_tag="VIS73_05110"
     CDS             5951..>6687
                     /gene="dnaN"
                     /locus_tag="VIS73_05110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY5762564.1"
                     /translation="MLLVKAGKEQLLGPLQSVSGIVERRHTLPILSNVLIERKGARLT
                     YLATDIEIQIRTGSETGEGNDGAVTVGARKLQDILRSLPEGVDVSLLIEEKRLQVKAG
                     RSRFNLQTLPADDFPRMLLTQDETVKLTVSQKAFKGLIAQTQYAMAAQDVRYYLNGML
                     LMIEASEVRAVSTDGHRLAFASVALTEKLGKREMILPRKTVLELQRLLADSDEPLTIE
                     AGNNQVRFSFGEIELVSKLIDGKVPDYT"
BASE COUNT         1230 a         1884 c         2239 g         1334 t
ORIGIN      
        1 tatggtcatc gtagtcgcca tacgcgtact ggcgcatcag cagctcttcg gagaggaagt
       61 gcgcacgcga gtaatcggcc agcgcctcga tgctctctgc gacgtcgcgg ttttcggcta
      121 gtcctgcctc gaccgtctgg atcaggcgca actgcacctg atgctcgcgt tcgatgtact
      181 tggcgtctgt cgccgcgggg ttctgcgtga tcatcaagtt ctcctggcag ctgcgaccat
      241 gaaggcgggc ggtttacgtc gagccattct acgcaagcct tgcgctcatt taccgatgca
      301 gaagccggaa aagatctcgc cgagcaagtc ctcggtggag tactcgccgg taatttcctg
      361 gagtgcgcgg tgcgccagcc gcagctcctc ggcgaacaac tcaagctggg aagcactgcc
      421 tcgcgcgtcg ctcaggtggt cggcacaacg tcgcagggca tcgaggtggc gttcgcgggc
      481 aatgaaggtc gcctcgcccg ggtgccaatg ggcgattgcg agcaggcgct cctcgagggc
      541 cggcaagccc tcgccggtcc gggcggatac tgcgagcgcg gccgcttcag ttgaatgcgc
      601 cgtcttggcg agatccgact tgttgaggac cgtgaggact tgcaggccgg gaggcagacg
      661 atcaatcagc gcccggtctt cgggttgaac gccagtgcgt gcgtcggcga taaccagggc
      721 gacgtcggcc ttgctgattt cccgccaggt ccgggcaatg ccgatctgct cgaccggatc
      781 gtcggtgtcg cgcaagccgg cggtatcgac cacatgcaca gggatgccgc gcagctgtac
      841 ggtgcgcttc aggctgtcgc gcgtcgtgcc ttcgtattcg ctgacgatcg cgatctcgtc
      901 gccggccagg tggttcatga gggaggactt gccgacgttg gggcggccga tgagaacgac
      961 gctgagacca tcgcgcagca gcttgccgcg gtcggcgtca cggcgcagcg cggtcaggtt
     1021 cacctcgatc tgttcaacgg cgcgttgggc ggcatcgggg gacagggcgt cgatctcctc
     1081 gtcggagaag tcgagggtcg cttcggtcag catccgcagg cgaacgagtt cgtgcgcgac
     1141 ggcggctatc gcctccgaga aggcaccgct gagcgagcgg atcgcgctcc gtgccgccgc
     1201 cgcggtcgag gcgtcgatga ggtcagcgat cgcttcggcc tgcacgaggt cgagcttacc
     1261 gttgaggaag gcacgctctg agaattcgcc ggggcgtgcc gggcgggcgc cgagctggtg
     1321 acagcgcgcc agaagttgcg cgatggcgac tggactgccg tggccgtgca gctcgagcac
     1381 gtcttcgccg gtgaaggaat tgggggcggt aaaggcaatc gccaggccgt cgtcgatggt
     1441 ctcaccggcg gcatcgcgaa agtggcagcg ggttgccaga cgcggtgcga gctgtgcgcc
     1501 aatcagcgca gcggcaaagc ggtcgagtcc ggcccccgaa atgcggacaa tgccgacgcc
     1561 accacgcccg ggcggcgtgg cgacggcggc gatggtgtcc ctatttgccg gatcggtcct
     1621 tgccaccttc gatcattcgc gtgatctgcc actgctgtcc gatcgacagg atgttgttga
     1681 ctacccagta gagcaccagt ccggccggga agaagaagaa catcacgccg aagacgaagg
     1741 gcatcgccat catgatcttg gcctggattg ggtcgggcgg cgtcgggtta aggcgcatct
     1801 ggatgatcat cgagacgacc atcaccaccg gcagcacgta gtaggggtcg gcgcgtgaca
     1861 ggtcgtgaat ccacaggatc cacggagcgc cgcgcatctc gacggcgccg agcagcaccc
     1921 agtagagcgc gatgaagacc ggaatctgga tcaggatcgg caagcagccg ccgagtgggt
     1981 tgatcttttc cttgcggtac agctccatca tttcctggtt catccgctgc cggtcgtcgc
     2041 cgtagcgttc cttgatctgc atcatccgcg gtgtcatctg gcgcatccgc gccatcgaac
     2101 ggtaggaggc cgccgacagc gggaagaagg cgagcttgat caggattgtc aggatgacga
     2161 tcgcccagcc ccagttgccg acgaagccgt ggatgaaatc gagcatccag aagatcggtg
     2221 cggcgatcac cgtcagccag ccgtagtcga cgaccagatc taggccgggc gcgagcttct
     2281 ccagcgttgc ctgttcctgc gggccggcgt agagcgtcat cgtcgcctcg gcgctactgc
     2341 ccggcgcgat ctcggccacc gggacgatga cccctgccgt gtataggtct ccctcgagct
     2401 tgcgcatgaa gaactcgcgc gcgccggtcg ccggcggcag ccaggcggcg acgaagtaat
     2461 gctggaccat ggcgatccag ccgtcctgag tgatggtcgg gaacttggcg ccgccgccgg
     2521 cgatatcgtc gaaactgatc ttcttgaact tctcgacctc ggtgtagatc gccgggccgg
     2581 tgtaggtcat cgtcatcgac gactcgccgg ggggcgaacg gccgtcgcgc tggaactggt
     2641 aataggcgtg tgtcgtcagt ggggtcgtac cgcgattgtc aatctgctgg gtgatcaaca
     2701 cccggtaatc gccacgtcgg aactcgtagc gtttgcgaac aacgacgccg ggaccgccat
     2761 cggcttcaag gtcgacgacc agtgactggc ggtcgccgag ttcggtttcg ttgctgacga
     2821 tccggaaatt tgcgcggtgc gtcggcagac cgtcgccgat caagcccgat tggatcgcat
     2881 agcggtggtc ggggtcgacc aagagcaagt tgcggtccgg cgattcggtg gcgcggtgat
     2941 cgagcaactc cagccgaagg atttcgccgc cgatcggcga gatgtcggcc accagcttgt
     3001 cggtgcggac gcggatcggt ttgggggttt cggcggccgc tgtggcgctg gccgccggga
     3061 cctgctggct gcccggcgcg gcaagggtcg ggttcggtgt gctcagggcg tcggaaaccg
     3121 tcggcgcgcc ggtggcaaca gccggcgccg cggtcgttgc ggcgggcggc ggtgctggat
     3181 tgttgtagcg ctcccattcg gtccacagca tgtagatcga caggaaaaat acggcgagga
     3241 tgatgaggcg gcgggtgtcc attggtgcct tgtcttcggt gtgccgttca gggaacgggg
     3301 tcgtagcccc cgtcgtgcca cggatgacag cgggagatgc gtttgagggt gagccaggaa
     3361 ccgcgcgtgg cgccgtggcg agtcagcgct tcgaccgcgt attccgaaca gcttggcgcg
     3421 tggcggcagc ttggcggcag aaacgggctg atcatcaagc ggtagccctt aaccagcacg
     3481 atcaatagtc gtttcatgtc ggtctccgca gcgtttgatt caatttggcg agcaggctgt
     3541 cgaggtcaag gcgaaggctt tcacgcgtct cggcttcgag ctgttgcggc aggtttcgga
     3601 ccaggcgaac caccagatcg actgacccga ggtctgtgcg gacggcgcgg aaacgttctc
     3661 ggattaggcg tttcaggaaa ttgcggcgcg tcgccagacg acagaagcgt tttccgatga
     3721 ccagtccgat tcgggccttc tcgtcgtccc cgtccccgcg ttgacgcaga tggatgttca
     3781 tccagcgccc tttgaggcgg cgtcggcttg cgaaggcctg gcggaaatgc tccgggtctg
     3841 ccagccgtgc cgcccgtggc aggcgcttgc tgcccgccac cgtgcgtcag gtcagaccga
     3901 caggcggcgg cggcccttgg cacgccgggc ggctaggacc gatcgtccgc cgcgggtgcg
     3961 gctgcggacg agaaaaccgt gggtacgctt gcgtcggatg accgacggtt gataggtgcg
     4021 cttcatgatt gacaaaccct tgaggacaaa acaaaaaggg cgcaattaga ccgtgcggtg
     4081 caggtactgt caagctgcgt ttgcgcgcgg gcttttttgg cggactgcgt cctggttcgg
     4141 tccaagcgcg cgcggggttg gcgggagggg cttgcgggcc tgtggataac tctagcgaag
     4201 gggcgtagaa tcgccgctct gctcagtcgt ccgttctcgc tcgcgcgctg tttccgccgg
     4261 ggaaacccgc gacgcttgtg gcatccgcgc cggcgccggc accaaataag aaagttagtc
     4321 tgactcgcaa tggaagtctt ctggaatacc tgtctttcgc agcttgccga ggagatgccg
     4381 gcccagcagt tctcgacctg gatcaggccg ctgacgatgg aacccgttac cgacggtaac
     4441 ggcaagcgct tgcgcatcgt ggcgcccaac ggcttcatcc tgaaatgggt acgcgagcgg
     4501 ttcctggctc gaatcgcgtc gatggccgag agccattttg gcgagccggt tgtaattgac
     4561 atcgtcgccg gcggtgctgt agccagcggc gaacgtaaac gggaagcggc tgtggctttg
     4621 gcggaggccc cggttgctgc ggcacgtcag gccgccgttc cgatgattaa cggagtcgcc
     4681 gcggccaacg gcacggagcc tgaccggggg cggctgatca atggctttac cttcgacaac
     4741 ctcgtggtag gcaaagccaa tgatctggct cgagctgccg ggatgcaggt cgccgagcat
     4801 ccgggtgggg cctataaccc gctgttcgtc tacggtggtg ccggacttgg aaagactcac
     4861 ctcattcacg caatcggcaa tttcatagcc gatcaatcgc cgagcaagtc gattcgttat
     4921 atccacgccg aggactacta caacgacgta gtgcgtgctt accagcagaa ggcttttgac
     4981 gccttcaagc gctcctatcg ttcggtcgat gtcctgttgc tcgacgacgt gcagtttttc
     5041 aacgggaaga accggtccca ggaagagttc ttttttcttt ttaattcgat gatcgaagcc
     5101 cgcaaacaga tcgtcattac ctgcgatacc tatccgaagg acatcaacgg tcttgaagac
     5161 cgcctgatta cccgctttga ctgggggctc acggtgcaga tcgagccacc ggagctggag
     5221 atgcgggtgg cgatcatcaa gaagaaggca gaagggaacg gaattgcgct tccggacgac
     5281 gttgccttct tcatcgcgaa gcatcttcgc tctaatgtgc gcgagcttga aggagcgctc
     5341 aatcgggtac tggcctatgc ccggtttcac ggtcaggcga tttcggttga attgagcaag
     5401 gacgcgctca aggatgtcgt cggggccttc aaccggcaga tcaccatcga caacatccag
     5461 aagacggtcg ccgactattt caatattcga gttggcgatt tgtattcaaa gaagcgggcg
     5521 cggatcatcg ttcgtccacg gcaggtggcg atgtggcttt gcaaggaggt tacccctcac
     5581 agttatcccg tgatcgggga agccttcggc gggcgcgacc acacgaccgt gattcatgcg
     5641 gtgcgcacga ttgaagcgca tcgcgcgaag gacggtgagc tgaatcacgc gctgcatgta
     5701 ctgctgcagg ttctgaaggg gtaatgcctt gatcgaagga taaggttggg gataagccat
     5761 tggcggtttc gggataactc cgggtttgga ggattccgag aagttgttca tcggcgctca
     5821 acagggctca aggtaggtgt ttacaagcgt ttattggttg taagttaatg tataagaacg
     5881 aaaaaagtac gttacccaca gaattggctc ttgcttcttc tttttcttct attgatttaa
     5941 aggaaaacca atgctgttag tcaaagctgg aaaggaacag cttctgggac cgctgcagtc
     6001 ggtttctggg atcgtcgaac gtcggcatac gctgccgatt ctttccaacg ttctgatcga
     6061 gaggaaggga gcgcggctga cctatctcgc gaccgatatc gagatccaga ttcgcaccgg
     6121 ttctgaaacc ggggaaggca acgacggtgc cgttaccgtt ggcgcgcgga aactgcagga
     6181 cattcttcgc tcgcttccgg agggagtcga tgtttcgctg ttgatcgagg agaagcgctt
     6241 acaggtaaag gcgggtcgga gcaggttcaa tttgcagacg cttcccgccg acgatttccc
     6301 gcggatgctg ctgacgcagg atgagacggt caagttgacg gtttcgcaga aggccttcaa
     6361 gggcctgatc gcccagacgc agtatgcgat ggctgcccag gacgtccgct actacctgaa
     6421 cgggatgttg ctgatgatcg aggcgagtga ggtgcgtgcg gtctcgaccg atggtcatcg
     6481 gctggcgttc gcctcggtgg cgctgaccga gaagctgggc aagcgggaga tgattctgcc
     6541 gcggaaaacc gtcctcgagt tgcagcgcct gctggcggac agcgatgagc ctctgacgat
     6601 agaggccggc aacaatcagg tccgtttttc gtttggtgag atcgaactgg tttcgaagtt
     6661 gatcgacggt aaagtcccgg actacac
//
LOCUS       DATDAA010000157        10399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_150994,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000157 DATDAA010000000
VERSION     DATDAA010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10399
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150994"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /gene="ruvC"
                     /locus_tag="VIS73_05115"
     CDS             <1..505
                     /gene="ruvC"
                     /locus_tag="VIS73_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY5762565.1"
                     /translation="DPGLRATGFGLIDRSGQRLAYVASGVIRSSDKDELPARLATLFR
                     GISEVIATYAPAVCAVEKVFVNVNPQSTLLLGQARGAAIAALVHAERPVFEYTALQVK
                     QAVVGHGKAAKEQVGFMVRRLLTLSGEPAADAADALACAICHAHSLGMAASGVTNTRR
                     RGGRLLG"
     gene            502..1443
                     /locus_tag="VIS73_05120"
     CDS             502..1443
                     /locus_tag="VIS73_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEY5762566.1"
                     /translation="MNTGTAAVMPVVAFAARAIRAPFLSVTAIALAIGFALAARDAVA
                     LPWLTIGVAALGALLAHAGANVLNDWADELNGSDRANEERISPFTGGSRLIQEAQVDA
                     ATTRRLAWSLLVAASLCGLALLPSLGAPLLAFGAAGLGLAWAYSSPPLQLMARGLGEI
                     AVGLAWATVIAGADFVVRGAASPTPWLAGAGFALMIVALLWVNEFPDVAADRQAGKRN
                     AVVRLGAYAATRAYPLLPLSAAGTTLLAIIAGALPAAAAIACAGLLPALAAARRLRQA
                     LGARGEARHATLTTAVKANLAAVHAYGILLAAALLLA"
     gene            1461..2042
                     /gene="ruvA"
                     /locus_tag="VIS73_05125"
     CDS             1461..2042
                     /gene="ruvA"
                     /locus_tag="VIS73_05125"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY5762567.1"
                     /translation="MIGRLSGTLLAKHPPQLLIDVGGIGYEVEAPMSTVFQLPECGKP
                     VVLLTHLIVREDGHFLYAFLTEGERALFRQLLKISGVGARIALALLSGMSVDELAGAV
                     ARQETGRLTKVPGIGKKTAERLLLELKDKLPSSLGAPTGARQAADPATDILNALLALG
                     YNDREAGAVLRQLPPELAVADGIRQALKLLAKA"
     gene            2057..3103
                     /gene="ruvB"
                     /locus_tag="VIS73_05130"
     CDS             2057..3103
                     /gene="ruvB"
                     /locus_tag="VIS73_05130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY5762568.1"
                     /translation="MIETDDLSPERLTSPEPASTQEEAIERALRPKRFDEYVGQEKIR
                     GQLSIFIEAARRRREALDHVLLFGPPGLGKTTLAHIIAHEMGVNLRQTSGPVLERAGD
                     LAAMLTNLEPHDVLFIDEIHRLSPVVEEILYPALEDFQIDIMIGEGPAARSVKLDLPP
                     FTLVGATTRAGMLTNPLRDRFGIVARLEFYSAEELSRIVDRSARLLGASVDADGALEV
                     ARRARGTPRIANRLLRRVRDFAEVRADGAVTLGVADAALTMLEVDSAGLDILDRKFLR
                     AIIDRFAGGPVGVDNLAAAVGEARDTLEDVVEPFLIQQGYLQRTPRGRMATPSIYRHF
                     GITRPDDPPELWRE"
     gene            3100..3546
                     /gene="ybgC"
                     /locus_tag="VIS73_05135"
     CDS             3100..3546
                     /gene="ybgC"
                     /locus_tag="VIS73_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEY5762569.1"
                     /translation="MKNPDRRETPFSIEARVYYEDTDAAGVVYYANYLRFFERCRTEW
                     LRAIGHDQSELAREHNLAFVVRRVTAEYRRPARLDDRLRIELVASIEGRARLRCEQRA
                     LRVDADGKGELLVEASVELACISVVDFRPVPIPAWLSDQLPRAHSR"
     gene            3543..4226
                     /gene="tolQ"
                     /locus_tag="VIS73_05140"
     CDS             3543..4226
                     /gene="tolQ"
                     /locus_tag="VIS73_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEY5762570.1"
                     /translation="MNLTQDLSILSLILEASAVVQAVMVLLASVSFMSWYYIFRKAFA
                     VKDSRRLTERFEREFWSGADLNVLFKRTQSDRLHAGPMARIFESGLAEFMKLRGQKGL
                     EPADVVAGGRRAMQATRQREMDNLEAHLSFLASVGSVSPYIGLLGTVWGIMHAFRGLA
                     NVGQATLASVAPGIAEALVATAIGLFAAIPAVVAYNRFVHDIDRLDNRFESFMEEFSN
                     ILQRQTRRD"
     gene            4235..4648
                     /gene="tolR"
                     /locus_tag="VIS73_05145"
     CDS             4235..4648
                     /gene="tolR"
                     /locus_tag="VIS73_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEY5762571.1"
                     /translation="MAKQRRSMAQINVVPYIDVMLVLLVIFMVTAPMIQLGTVDLPTV
                     GKSQSLPVKPIEITVRADRSLMFRDHDSGGKDREVSSRELTQLVRAAQKKNPEQPVLI
                     AGDKAVAYAAVLAVMDQLQKQDVRRVGLLVQPGLN"
     gene            4648..5493
                     /locus_tag="VIS73_05150"
     CDS             4648..5493
                     /locus_tag="VIS73_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY5762572.1"
                     /translation="MNSVTAISLDNERFATPVEPGKWAAIALSVFMHGLLIGSLLVAV
                     QWQTRHVPLQAELWREIPREPSPPPKAPEPRPKPVEKPVPPPPKPEPAPTPVVPEKTV
                     PVIEPPKPKPPDIAVAKEKPKPPPKPKPAPKPKVVEKPRPPDFASELAAAEREIARHR
                     ETQEKTAAAEREAKALADARAEAAASARARAEAEYADRIRSKIRGNIVLPPRIQGNPE
                     AEFAVVQLPDGVVLSVRLLRSSGIPLLDQAIERAIMKSSPLPLPSLPELFRRDLRLKY
                     RPYET"
     gene            5573..6862
                     /gene="tolB"
                     /locus_tag="VIS73_05155"
     CDS             5573..6862
                     /gene="tolB"
                     /locus_tag="VIS73_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEY5762573.1"
                     /translation="MRTSIISWLRRGALLTFLLSLTSGASAQLAIEITGAGAFRIPIT
                     VANFSGDPLVAAQIVSVVRADLERSGLFRHVDYGVEPVPETALATYDLWKSRGADAFV
                     AGSIVPRPDGRIEARFRVYDTIRKAPLGGVAFVTSADALRATAHRIADYIYEKLMGEP
                     GVFSTRIAYVVKRSDRWELQIADADGFGAQAALVSREPIISPSWAPDGKALAYVSFED
                     QKPIVYVHSLASGQRRIVANFKGSNSAPAWSPDGRRLAVVLTKEGGSQLFVINADGTG
                     LLRLASSPGIDTEPYFSHDGAWIYFTSDRGGSPQIYRIPSSGGTAVRVTFEGSYNVTP
                     RISPDGKTLAYISRREDGSFQLSIMDLATLQIQILTDSRKDESPSYAPNGRMIVIATE
                     IGGRGVLTAVSSDGRIKQRLSTTAGDVREPAWGPFVR"
     gene            6936..7499
                     /gene="pal"
                     /locus_tag="VIS73_05160"
     CDS             6936..7499
                     /gene="pal"
                     /locus_tag="VIS73_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY5762574.1"
                     /translation="MKANVRTILMLSFAALVAVGCATQPKEEPAPAPAPAPAPAPRAV
                     TPAEPAPVAPAPSLSPTDLTNPANLTNPESILSKRKVYFDYDKYDIKPEFQDLVAAHG
                     KLLAANPQLKVLLQGNADERGSREYNLALGQKRAEAVRKAVQLLGARDSQIEAVSLGE
                     EKPVCVQSSEDCWWQNRRTDILYSGEY"
     gene            7499..8284
                     /gene="ybgF"
                     /locus_tag="VIS73_05165"
     CDS             7499..8284
                     /gene="ybgF"
                     /locus_tag="VIS73_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY5762575.1"
                     /translation="MRVRGLVLLSLALLSHPAAAGLFDDTEARRQIAELRAETAQAKE
                     LQAQAKARLETLENALRMQLELANQIESLRGELARLRGQVEVLQHESATGQKRQRDYY
                     VDLDTRLRALETAATNAARLAGSAQAQVPSPTEKSDPATITRDYESALALFRDAKYKE
                     SHTAFSAFVGSYPDDPLAPSAQYWLGNSLYALRDCKKAIDAQELLLKQWPESSKAPDA
                     MLAIGTCQRELGNAKSSRKTLEALIAKHPGTPAAAKAKDRLKK"
     gene            8284..8934
                     /gene="queE"
                     /locus_tag="VIS73_05170"
     CDS             8284..8934
                     /gene="queE"
                     /locus_tag="VIS73_05170"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEY5762576.1"
                     /translation="MNSESTLRVTEIFFSLQGETSRVGLPTVFIRLTGCPLRCRWCDT
                     AYAFTGGTTRTLSDILGEVLQHPTRRVTVTGGEPLAQADCHLLLTRLADAGCDVSLET
                     SGALDISMVDRRVSRIVDLKPPGSGESSRNRLENLRELTPRDEVKFVIADREDYEWSR
                     NLIREYEIAARCPVLLSPVHDKVEPAQLAGWVLADGLEVRLQVQLHKLLWGGSPGH"
     gene            8963..9346
                     /locus_tag="VIS73_05175"
     CDS             8963..9346
                     /locus_tag="VIS73_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY5762577.1"
                     /translation="MGPSTDDGDQAQRRHFSRIHFQSRATLSGEGRSLPCTLIDLSLR
                     GALVDCADAGHSISDQVLLDLDLDGSHHIRIEGEVSHRQGDRIGISCTGIDIDSMTHL
                     RRIVELNTGDGALLNRELAALLASH"
     gene            9354..10061
                     /gene="queC"
                     /locus_tag="VIS73_05180"
     CDS             9354..10061
                     /gene="queC"
                     /locus_tag="VIS73_05180"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEY5762578.1"
                     /translation="MTASAVVLLSGGLDSATTLAIAQNQGFTCHCLSFDYGQRHRAEL
                     EAAGAIARSLRAATHRLFTLDLAQFGGSALTDSQISVPTDGTTAGIPVTYVPARNTVL
                     LSLALAFAETLRARDIFIGINAVDYSGYPDCRPEYLRAFQAMADLATREGVGGRTITI
                     HAPLIEMSKADIIRCGSALNVDYASTVSCYQADANGNACGSCDACRLRAIGFREAAIS
                     DPTRYQAHAVKPLDTSR"
     gene            10144..10219
                     /locus_tag="VIS73_05185"
     tRNA            10144..10219
                     /locus_tag="VIS73_05185"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10177..10179,aa:Lys,seq:ttt)
BASE COUNT         1788 a         3573 c         3247 g         1791 t
ORIGIN      
        1 cgacccgggc ctgcgcgcca ccggctttgg cctgatcgac cggagcggac aacggctcgc
       61 ctatgtggcc agcggcgtga ttcgcagcag tgacaaggac gagttgccgg cgcggctggc
      121 gacgctgttc cgcggcatct cggaagtgat cgccacctac gcgccggccg tctgcgccgt
      181 cgagaaggtc ttcgtcaacg tcaacccgca gagcacgctg ctgctcgggc aggcacgcgg
      241 cgcggcgatt gcggctctgg tgcacgccga gcgcccggtc ttcgaataca ccgcgctgca
      301 ggtcaagcag gcggtggtcg gccacggcaa ggccgccaag gaacaggtcg gcttcatggt
      361 gcgacggctg ctcacactgt caggcgagcc ggccgccgac gccgccgatg cgctggcctg
      421 cgcgatctgc cacgcgcaca gcttgggcat ggcggcgagc ggcgtgacga acacgcgccg
      481 gcgcggcgga cggctgctcg gatgaacacc ggcaccgccg ccgtgatgcc ggtcgtcgcc
      541 ttcgccgcac gcgcgatccg tgcgcccttc ctcagcgtca ccgccatcgc gctggcaatc
      601 ggctttgcgc tcgcggcgcg cgacgccgtg gcgctgccct ggctaacgat aggcgtcgct
      661 gcgctcggcg ccctgctcgc ccacgccggc gccaacgttc ttaatgactg ggctgacgaa
      721 ctgaatggca gcgaccgcgc caacgaggag cgaatctccc cattcaccgg cggcagccgc
      781 ctgattcagg aagcgcaagt cgacgcggcg acgacgcgcc ggctggcctg gtccctgctg
      841 gttgccgcgt cactgtgcgg actggccttg ctgccgtcac tgggcgcgcc gctgctcgcc
      901 tttggggccg cgggtcttgg tctggcctgg gcctattcct cgccgccgct gcaactgatg
      961 gcgcgcgggc tcggcgagat cgccgtcgga ctggcctggg cgaccgtcat cgccggtgcc
     1021 gatttcgtcg tgcgcggcgc cgcctcgccg acgccctggc tggccggcgc gggcttcgcg
     1081 ctgatgatcg tcgcgctgct ttgggtcaac gagttccccg atgtggccgc cgaccgccag
     1141 gccggcaagc gcaatgccgt ggtccgcctc ggcgcgtacg ccgcgaccag agcctatccg
     1201 ctactgccgc tgagcgccgc cggcacgacc ttgctggcga tcatcgccgg cgcgctgccc
     1261 gccgcggccg cgatcgcctg tgccgggctg ttgccggcgc tcgccgcggc tcgccgcctt
     1321 cgccaggcgc tcggcgcccg gggcgaagcg cgccacgcca cgctcaccac cgcggtcaag
     1381 gccaatctgg cggcagtaca cgcttacggc atactgctcg ccgccgcgct cctgctcgcc
     1441 taagtaaaga aagccagacg atgatcggaa gactctcggg aaccctgctc gccaagcatc
     1501 cgccgcagct cttgatcgac gtcggcggga tcggctatga agtcgaggcg ccgatgagca
     1561 cggtcttcca gctgcccgaa tgcggcaagc cagtggtgct gctgacgcac ctgatcgttc
     1621 gcgaggacgg tcatttcctt tacgcctttc tcaccgaggg cgagcgtgcg ctgtttcgcc
     1681 agttgctgaa gatctccggc gtcggcgccc gcatcgcgct ggcgctgctc tctggcatgt
     1741 cggtcgacga actcgccggc gcggtcgccc gtcaggaaac cgggcgtctg accaaagtac
     1801 cggggatcgg caagaagacc gccgagcgcc tgctgctcga gctgaaggac aagctgccga
     1861 gttcgcttgg cgcaccaacc ggcgcgcggc aggccgccga tccggcgacc gacatcctca
     1921 atgccctgct ggccctcggc tacaatgacc gcgaagccgg cgcggtgttg cgccagctgc
     1981 cacccgagct cgccgtcgcc gacggcatcc gccaggcgct gaagctgctc gccaaggcct
     2041 gatcggccgg catccgatga tcgaaaccga cgacctttcc cccgaacgac tgacttcgcc
     2101 cgaaccggct tccacccagg aagaggcgat cgagcgcgcg ctgcggccga agcgtttcga
     2161 cgaatacgta ggccaggaga agatccgcgg ccagctgtct atcttcatcg aggccgccag
     2221 gcggcgccgc gaggcgctcg atcacgtcct cctgtttggc ccgccggggc tgggcaaaac
     2281 aacgctggcg catatcatcg cccacgagat gggggtgaac ctgcggcaga cctcgggccc
     2341 ggtactcgaa cgggccggcg acctggcggc gatgctgacc aatctggagc cacacgacgt
     2401 gctgttcatc gacgagatcc accgtctgtc gcccgtggtc gaggaaatcc tctatccggc
     2461 gctcgaggat tttcagatcg acatcatgat cggcgagggc ccggcagcgc gctcggtcaa
     2521 gctcgacctg ccgcccttca cgctggtcgg cgcgaccacc cgcgccggca tgctgaccaa
     2581 tccgctgcgc gaccgtttcg gcatcgtcgc ccggctcgag ttctacagtg ccgaggagct
     2641 gtcgcgtatc gtcgaccgtt cggcgcgact cctcggcgcc agtgtcgatg ccgacggtgc
     2701 gctcgaagtg gcgcggcgcg cccgcggcac gccgcggatc gccaaccgcc tgttgcgccg
     2761 agttcgcgac ttcgccgagg tgcgtgccga tggcgcggtc accctcggtg tcgccgacgc
     2821 cgcactgacc atgctcgaag tcgactccgc cggactcgac atcctcgacc gcaagttcct
     2881 gcgcgcgatc atcgaccgct ttgccggcgg tccggtcggc gtcgataacc tcgcggcggc
     2941 cgtcggcgag gcgcgcgata cgctcgagga cgtggtcgag cctttcctga tccagcaggg
     3001 ttacctgcaa cggacgccac gcgggcgaat ggccacgccc tcgatctatc ggcacttcgg
     3061 catcacccgc cccgacgacc ccccggagct ctggcgcgaa tgaagaaccc ggaccggcgc
     3121 gaaacgccct tctccatcga ggcgcgtgtc tattacgaag acaccgacgc cgctggcgtc
     3181 gtctactacg ccaattacct gcgcttcttt gaacgttgcc gcaccgaatg gctgcgtgcg
     3241 atcggccacg accagtcgga actggcgcgc gaacataatc tggccttcgt cgtccgccgc
     3301 gtcaccgctg agtaccgtcg cccggcccga ctcgatgacc ggctgcgtat cgaactcgtc
     3361 gccagcatcg agggccgcgc gcgactgcgc tgcgagcagc gtgccctgcg cgtcgatgct
     3421 gacggcaagg gcgaactcct ggtggaagcc agcgtcgaac tggcctgcat cagcgtcgta
     3481 gacttccgcc ccgtgccgat cccggcctgg ctttccgatc aactgccccg cgcccattcc
     3541 cgatgaacct tacgcaagac ctttcgattc tctcgctgat cctcgaggcg agcgccgtgg
     3601 tccaggccgt catggtcctg ctggcgtcgg tctccttcat gtcctggtat tacattttcc
     3661 gcaaggcgtt cgcggtcaag gattcgcgca gactgaccga gcgcttcgaa cgcgagttct
     3721 ggagcggcgc cgacctcaac gtgctgttca agcgtacgca atccgatcgc ctgcacgccg
     3781 gtccgatggc gcgcatcttc gaatcgggac tcgccgaatt catgaagctg cgcggccaga
     3841 agggcctcga gccggccgac gtcgtcgccg gcggacgccg cgcgatgcag gcaacccggc
     3901 agcgcgagat ggataacctt gaggcgcacc tttcctttct cgcttcggtc ggttcggtct
     3961 cgccctacat cggtctgctc ggcacggtct ggggaatcat gcatgccttc cgcggcctgg
     4021 ccaacgtcgg ccaggcgacg ctcgcctcgg tcgccccggg catcgccgag gcactggtgg
     4081 caaccgcaat cggcctgttc gcggcgattc cggcggtggt tgcctacaac cgcttcgtcc
     4141 atgacatcga ccggctcgac aaccgcttcg agagcttcat ggaggagttc tcgaacatcc
     4201 tgcagcgtca gacgcgccgc gactagggca aaccatggcc aagcaacgcc ggtcgatggc
     4261 acagatcaac gtcgttccct acatcgacgt catgctggtg ctgctggtga tcttcatggt
     4321 cacggcaccg atgatccaac tcgggaccgt cgatctgccg acggtcggca agtcgcagtc
     4381 gctcccggta aaaccgatcg agatcaccgt cagggccgat cgcagcctga tgttccgtga
     4441 ccatgacagc ggcggcaagg accgcgaggt cagttcacgc gaactcaccc agctggttcg
     4501 cgcggcgcag aagaagaacc ccgaacagcc cgtgctgatc gccggcgaca aggccgtcgc
     4561 ctacgcggcg gtgctcgcgg tcatggacca gttgcagaag caggacgtcc gccgcgtcgg
     4621 cctcctggtc cagcccggtc tcaattgatg aacagcgtca ccgcgatttc gctcgataac
     4681 gagcgattcg ccacgccggt cgaacccggc aagtgggccg cgatcgccct cagcgtcttc
     4741 atgcatggcc tgctgattgg ctcgctgctg gtcgcggtgc aatggcagac ccgccatgtg
     4801 ccgctgcagg cagagctctg gcgcgagata ccgcgcgagc cgtcgccacc gccaaaggct
     4861 cccgagccac gccccaagcc ggtcgagaag ccggtcccgc cgccaccaaa gccggaaccg
     4921 gcgcccaccc cggtcgtgcc cgaaaagacc gtgccggtaa tcgagccgcc caagcccaag
     4981 ccacccgata tcgcggttgc caaggaaaag ccgaaaccgc cgccgaagcc aaagccggcg
     5041 cccaagccca aggttgtaga aaagccgcgt ccgccggatt tcgccagcga gttggcggcg
     5101 gccgagcgcg aaatcgcccg ccaccgcgag acccaggaga aaaccgctgc ggccgagcgc
     5161 gaggccaagg cgctcgccga cgcgcgtgcc gaggccgcgg ccagcgcccg ggcgagagcc
     5221 gaagccgagt acgccgaccg catccgcagc aagatccgcg gcaacatcgt cctgccgccc
     5281 cgtatccagg gcaaccccga ggcggagttc gccgtcgtcc agctgcccga cggggtcgtg
     5341 ctcagcgtgc ggctgctccg ctccagcggt attccgttgc tcgaccaggc gatcgagcgg
     5401 gcaatcatga agtcgtcgcc gctgccgctg ccctctctgc ccgagctatt ccgccgcgat
     5461 ctgcggctga aataccgccc ctacgaaacc tgagattggg cgaatcggcc cgccggcggt
     5521 tgatattcgt cgatctggta tagtcggacc ctccgaccac cctttgattg acatgcgcac
     5581 gagcattatt tcctggttgc gacgcggcgc gctgttgact ttccttcttt cgctgactag
     5641 cggcgccagt gcgcagctcg cgatcgagat caccggcgcc ggcgccttcc gcatcccgat
     5701 taccgttgcc aacttctcgg gcgatccgct ggtcgcggct cagatcgtct cggtcgtgcg
     5761 cgccgacctg gagcgcagcg ggctcttccg ccatgtcgac tacggcgtcg aaccggttcc
     5821 ggaaacggcg ctggccacct acgatctttg gaaatcgcgc ggcgcggacg ctttcgtcgc
     5881 cggcagcatc gtgccccgcc cggacggtcg catcgaggcg cggttccgcg tctacgacac
     5941 gattcgcaag gcgccgcttg gcggcgtcgc cttcgttacc tctgccgacg cgctgcgcgc
     6001 caccgctcac cgcattgccg actacatcta cgagaagctg atgggcgaac ccggtgtgtt
     6061 ctcgacgcgt atcgcctatg tggtgaaacg cagcgaccgc tgggagctgc agatcgccga
     6121 cgcggacgga ttcggcgcgc aggccgcgct ggtgtcgcgc gagccgatca tttccccgtc
     6181 ctgggcgccc gacggcaagg ccctcgcgta cgtctccttc gaagaccaga agccgattgt
     6241 ctacgtgcat tcgctggcca gtggccaacg gcgcatcgtc gccaacttca agggctcgaa
     6301 ctcggcaccg gcctggtcgc ccgacggcag acgccttgcc gtcgtactga ccaaggaagg
     6361 tggctcccag ctcttcgtca tcaatgccga cggcaccggc ctgctgcgcc tggcgagttc
     6421 gcccggaatc gataccgagc cgtacttttc ccatgacggc gcgtggatct acttcacttc
     6481 agaccgtggc ggcagcccgc agatctaccg gattccgtct tcgggtggca cagcggtacg
     6541 cgtgaccttt gaaggatcgt acaacgtcac gccgcgcatt tccccggacg gcaagacgct
     6601 ggcttacatc agccggcgtg aggatggcag cttccagctg tcgatcatgg atctggcgac
     6661 gttgcagatt cagatcctca ccgattcccg caaggacgaa tcgccgagtt acgcacccaa
     6721 cggtcgcatg atcgtcatcg cgaccgagat tggcggccga ggcgtgctga cggcggtgtc
     6781 gagcgacggg cggatcaagc agcgcctctc gaccacggca ggtgatgttc gcgagccggc
     6841 ctggggaccg ttcgttcgct gaagtatcat tcgttcataa ttcgctgatg atttgctcgt
     6901 aatcccccac aggattttcc caaaaggaga aacacatgaa agctaacgtc cgtacgattc
     6961 tgatgttgtc gttcgccgct cttgtcgcgg ttggctgcgc cacgcaaccc aaggaggagc
     7021 cggccccggc tcccgcgcca gctcccgctc ctgcaccgcg cgctgtcact ccggctgaac
     7081 cggcaccggt cgcacccgca ccgagtctgt cgccgaccga cctgaccaac ccggcaaacc
     7141 tgaccaaccc cgagagcatt ctctcgaaac gcaaggtcta tttcgactac gacaagtacg
     7201 acatcaaacc cgagtttcag gacctggtcg ccgcccacgg caaactgctc gccgccaacc
     7261 cgcaactgaa ggtgctgctc cagggcaacg ccgacgagcg cggtagccgc gagtacaacc
     7321 tggcgctcgg acagaagcgt gccgaggccg ttcgcaaggc ggtccagctg ctcggcgctc
     7381 gtgactcgca gatcgaggcg gtcagccttg gcgaagagaa gcctgtttgc gtgcagagca
     7441 gtgaggattg ctggtggcag aaccgccgca ccgacatcct ctactccggc gagtattgat
     7501 gcgcgtcagg ggactcgttc tcctgtccct ggccctgttg tcgcaccccg cggcagcagg
     7561 gctttttgac gacaccgagg cgcgccgtca gattgccgag ttgcgggcag agacagcgca
     7621 ggccaaggag cttcaggcgc aagccaaggc taggttggag acgctggaga acgcgctgcg
     7681 catgcagctt gaactcgcca accagatcga atccctgcgt ggcgaactgg cacgactgcg
     7741 cggacaggtc gaggtcctgc agcacgaatc cgcgaccgga cagaaacgcc agcgcgatta
     7801 ttacgtcgac ctcgacacgc ggctgagagc actcgaaacg gccgccacca atgcagcccg
     7861 acttgccggc agcgcacaag cgcaggtgcc aagcccaacg gaaaaatcgg atccggcgac
     7921 gatcacccgc gactatgagt cagcgctcgc actgttccgt gatgcgaaat acaaggagtc
     7981 gcacacggcg ttttcggcct ttgtcggcag ctacccggat gacccgctcg ccccttcggc
     8041 gcagtactgg ctgggaaact cgctctacgc gctgcgcgac tgcaagaagg ccatcgatgc
     8101 acaggaactg ctgctgaagc agtggccgga gagcagcaag gcgcctgacg ccatgctcgc
     8161 cattggcacc tgccagcgtg aactcggcaa cgccaagtcg tcgcgcaaga cgctggaagc
     8221 gctgatcgcc aaacaccccg ggacgccagc ggcagccaag gcgaaagacc gcctcaaaaa
     8281 gtagtgaatt cagaatcgac acttcgcgtc accgagatct ttttctcgct tcagggtgaa
     8341 acttctcggg ttggcctgcc gacggttttc attcgcttga ccggatgccc gctgcgctgt
     8401 cgctggtgcg acaccgccta cgccttcacc ggcggtacga cgcgcacgct gagcgacatc
     8461 ctcggtgaag tgctgcaaca ccccacccgg cgggtcaccg tcaccggcgg cgaaccactg
     8521 gcgcaggccg attgccacct gctgctaacg cgactggccg acgccggatg cgatgtatcg
     8581 ctggagacca gcggcgccct cgatatctcg atggttgacc ggcgggtgtc gcggatcgtc
     8641 gacctcaagc ctcccgggtc aggtgagtcc agccgaaacc ggctcgaaaa cctgcgggag
     8701 ctgacgccgc gcgacgaggt caagttcgtg atcgcggatc gcgaagacta tgaatggtca
     8761 agaaatctga ttcgcgagta cgaaattgcg gcgcgctgcc cggtgttgtt gtcgccggtc
     8821 catgacaagg ttgaaccggc gcaacttgcc gggtgggttc tcgctgacgg actcgaagtt
     8881 cgacttcagg tccagctgca caaactgctc tggggaggct cacccggcca ttgacgcggc
     8941 ctgcggcgta caattcccgc gcatgggacc ttccaccgac gacggcgatc aggcgcagcg
     9001 ccgccacttc tcgcggatac atttccagtc gcgcgctacc ttgagcggcg aaggccgcag
     9061 cctgccgtgc acgcttatcg atctgtcgct gcgcggcgcg ctcgtcgact gcgctgatgc
     9121 cggacattcg atcagcgacc aagtgctgct tgatctcgat ctcgatggca gtcaccacat
     9181 ccgtattgag ggagaagtca gccaccgcca aggcgaccgt atcggtatca gctgcaccgg
     9241 tatcgatatc gacagcatga cccacctgcg gcggatcgtt gaactgaaca ccggcgacgg
     9301 cgcgctgctc aaccgcgaac tcgcggcgct gctggcgagc cattgattcc ggcatgacag
     9361 ctagcgcagt cgtcctgctt tcagggggac tcgattcggc aacgacgctt gccatcgccc
     9421 agaatcaggg tttcacctgt cattgcctgt ccttcgatta cggacagcgg catcgagccg
     9481 aactcgaggc agcgggcgcc atcgcgcgat cccttcgggc tgcgacccat cgcttgttta
     9541 cgctagatct cgcgcagttt ggtggctcgg ccctgaccga cagccagatc agcgttccaa
     9601 ccgacggtac caccgccggc atacccgtga cctacgttcc cgcccgcaac accgtcctgc
     9661 tctcgctagc gcttgcgttt gccgagacgc ttagggcccg agacatcttc attggcatca
     9721 acgcggtcga ctattcgggt taccccgatt gccgacccga gtatttgcgg gccttccagg
     9781 ccatggccga cctcgcgacc cgtgagggcg tcggtggacg cacaatcacg attcacgcgc
     9841 cgctgatcga gatgagtaag gccgacatca ttcgttgtgg cagcgcactg aacgtcgatt
     9901 acgcatcgac ggtgtcctgc tatcaggccg atgccaacgg gaacgcctgc ggctcgtgcg
     9961 acgcctgccg cctgcgcgcg atcggttttc gggaggccgc gatcagcgat ccgacacgtt
    10021 accaggcgca cgccgtcaag cctctcgaca cttcccgctg aaccgcttcg cagaaggctt
    10081 ttcgggcccc atgtggtttg acagggcgag gccacctctc tataatccgc gcttcttttt
    10141 cgggggtcgg tagctcagtt ggtagagcag cggactttta atccgttggt cgcgagttcg
    10201 agcctcgccc gacccaccaa aagaattcga cggcttacct ctcggggtgg gccgttttca
    10261 tttgggcatt gcgggatgcc aacgggattg gcggtcagtt gatgctgggg ccgggtggat
    10321 gtcgagtgag atgcccaggt cgacattggg tcggcatcac aaacgtcaat gacaactgct
    10381 gtcaacgcac gtgacctag
//
LOCUS       DATDAA010000158         2501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_361527,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000158 DATDAA010000000
VERSION     DATDAA010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361527"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1911
                     /gene="pilB"
                     /locus_tag="VIS73_05190"
     CDS             196..1911
                     /gene="pilB"
                     /locus_tag="VIS73_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEY5762579.1"
                     /translation="MAANPDNVQISGLARAMVQAGRLREAEAESLLAQAASSNSSFIE
                     QLVATKRATALEVARFAADTFGYPLLDLSAFEVTNFPKDAIDRKLIATHKVLPLHKRG
                     NRIAVAVADPTNLRALDEIRFQTGLTVDPVIVEAPKLLPLAAKMSESAEDTLKSIASE
                     DLNLEFTDEASEAKAAEADTAEVDDAPVVKFIQKILLDAINEGASDIHFEPYEKFYRI
                     RFRVDGILREVAQPPLVIKEKVASRIKVISRLNIAEKRVPQDGRMKLVLSKSRAIDFR
                     VSTLPTIHGEKIVMRILDPSSATLGIEALGYDPDQRESLEKAIHRPYGMVLVTGPTGS
                     GKTVSLYTCLNILNEPDINISTAEDPCEINLAGINQVNVDDKAGLTFSAALKSFLRQD
                     PDIVMVGEIRDIETAEIAVKAAQTGHMVLSTLHTNDAPQTLTRLKNMGVPLFNIASSI
                     ILITAQRLARRLCTCKKPLDVPRETLINAGFKEADLDGSWQVYGPNGCDRCKGSGYKG
                     RVGIYQVMPINEAIQRIIMADGNSLDIAAQAQRDGVKDLRQSGLLKVRQGLTSLDEVL
                     STTNE"
     gene            1931..>2501
                     /locus_tag="VIS73_05195"
     CDS             1931..>2501
                     /locus_tag="VIS73_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5762580.1"
                     /translation="MVAAKKQDVVKQYAFTWEGKDKNGKIVRGEMRVPSQTVVQASLR
                     KQGILVTKIRKQSVGGGGKVTDKDLTMFTRQLATMMKAGVPLLQAFDIVGRGHNNPAV
                     GKLLLDIKQTVETGSSMSQAFRKYPQYFDVLYCNLVAAGEQAGILDTLLDRLATYKEK
                     ILAIKAKIKGALFYPTAVILFALVITAVIL"
BASE COUNT          546 a          809 c          726 g          420 t
ORIGIN      
        1 atccccgagg cgggagtgtc gctgaagatc ggcaacgtgc cgatcgaaat cgtgcagacg
       61 cagaaccgca gcgtgaaagt tgcccgtgta ttcatgccgt tagcctagca acttcgcgtt
      121 tctctttaca aagcgggttg cccgtagcat gcttcccttg atttttaacg gatatcttcg
      181 ataacggccc cggcaatggc agcaaacccc gacaacgttc agatcagcgg tctggcccgc
      241 gccatggtgc aggccggccg gctgcgcgaa gccgaagccg agtcgctgct cgcgcaggcc
      301 gccagttcga actcgagctt catcgaacaa ctggtcgcca ccaagcgcgc gaccgcgctt
      361 gaagtcgcgc gcttcgccgc cgacaccttc ggttatccac tgctcgatct ttccgccttc
      421 gaggtcacca acttccccaa ggacgcgatc gaccgcaagc taatcgccac gcacaaggtc
      481 ctcccgctgc acaagcgcgg caaccggatt gccgtcgccg tcgccgaccc aaccaacctg
      541 cgggcgctcg acgagatccg cttccagacc ggcctaaccg tcgatccagt catcgtcgag
      601 gcgccgaaac tgctgccgct cgcggcgaag atgtcggagt cggccgaaga cacgctgaag
      661 agcatcgcca gcgaggatct caacctcgag tttaccgacg aggccagtga agccaaggca
      721 gccgaggccg ataccgccga ggtcgacgac gcgccggtcg tcaagttcat ccagaagatc
      781 ctgctcgacg cgatcaacga aggcgcctcg gacatccact tcgagcccta cgagaagttc
      841 taccggatcc gcttccgcgt cgacggcatc ctgcgcgagg tcgcgcagcc accgctggtg
      901 atcaaggaaa aagtcgcttc gcggatcaag gtgatctccc ggctcaatat cgccgagaaa
      961 cgagtgccgc aggacgggcg gatgaagctc gttctgtcga aaagccgtgc catcgatttc
     1021 cgcgtcagca cgctgccgac gattcacggc gagaagatcg tcatgcggat tctcgaccca
     1081 agttcggcaa cgctcggcat cgaggcgctg ggctacgatc ccgatcagcg tgagtcgctg
     1141 gagaaagcca tccaccgccc ttatggcatg gtgctggtca ccggaccgac cggttcgggc
     1201 aagaccgtgt cgctctacac ctgcctcaac attctcaacg agcccgacat caacatttcg
     1261 acggccgagg acccctgcga aatcaacctc gccggcatca accaggtgaa cgtcgacgac
     1321 aaggccgggc tgaccttctc cgccgcactg aaatcatttc tgcgccagga tcctgacatc
     1381 gttatggtcg gcgagatccg tgacatcgaa actgcggaga ttgccgtcaa ggcggcgcag
     1441 accggccaca tggtgctgtc gacgctacac accaacgatg cgccgcagac attgacccgc
     1501 ctgaagaaca tgggcgtgcc gctgttcaac atcgcctcca gcatcatcct gatcaccgcc
     1561 caacggctgg cgcggcggct atgcacctgc aagaagccgc tcgacgtccc gcgcgaaacg
     1621 ctgatcaatg ccggcttcaa ggaagcggac cttgacggca gctggcaggt ctacggcccc
     1681 aacggctgcg accgctgcaa gggcagcggc tacaagggtc gcgtcggcat ctaccaggtc
     1741 atgcccatta acgaagcgat tcagcggata atcatggccg acggaaattc gctcgacatc
     1801 gccgcgcagg cccaacgcga tggcgtcaag gacctgcgcc agtcgggtct gctgaaggtc
     1861 aggcagggac tcacgtcgct cgacgaagta ttgagcacga ccaacgaata gacagaaacg
     1921 gacggtcgcc atggtcgcag caaagaagca ggacgtcgtc aagcagtacg ccttcacctg
     1981 ggaaggcaag gacaagaacg gcaagatcgt ccgtggcgaa atgcgtgttc ccagccagac
     2041 cgtcgtccag gcgtccttgc gcaagcaggg catcctcgtc accaagatcc gcaagcagtc
     2101 ggtgggcggc ggcggcaaag tcaccgacaa ggacctgacg atgttcacgc ggcagctggc
     2161 gacgatgatg aaggccggtg tgccgctatt gcaggccttc gacatcgtcg gccgtggcca
     2221 taacaacccg gccgtcggca agctgctgct cgacatcaag cagaccgtcg aaaccggcag
     2281 ctcgatgagc caggcattcc gcaagtaccc acagtacttc gatgtgctgt attgcaacct
     2341 ggtcgcggcg ggcgagcagg ccggcattct cgacacgctg ctcgaccgtc tggcgaccta
     2401 caaggaaaag atcctggcga tcaaggcgaa gatcaagggt gcgctgtttt atccgaccgc
     2461 agtcatcctc ttcgcgctgg tcattaccgc ggtcatcttg a
//
LOCUS       DATDAA010000159         4425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_151680,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000159 DATDAA010000000
VERSION     DATDAA010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151680"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VIS73_05200"
     CDS             complement(<1..214)
                     /locus_tag="VIS73_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762581.1"
                     /translation="MIACRLLVAALALAPGLAGANPAPTRSDAEPIQIIVLDRPPYYV
                     VRDGKPSGFLLLRAKRFIEKAKIPIGS"
     gene            complement(243..1013)
                     /locus_tag="VIS73_05205"
     CDS             complement(243..1013)
                     /locus_tag="VIS73_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5762582.1"
                     /translation="MIWTLRRRRSLRIAAGSLVLLLGAVSLRAGAAEVQLRMLFRDKP
                     PYSYLEKGEPKGFLYERTRKILDLAGVRASYEMLPPKRLFAEIESNHGPVCSFGWYRI
                     PSREAFAKFTRPIHQDRPHLVLARRQLADRLRAAGSLAAIAGDRSLSLAAADGVSYGP
                     DLDALIASFAGRVERMLTAPLQVAQNVAAGRADLMFIDQDDFDYLRASARDFPKEELL
                     ALSFPDMPPGLRRHILCSRQVSDELMERMDTAIMRVLP"
     gene            1049..3877
                     /locus_tag="VIS73_05210"
     CDS             1049..3877
                     /locus_tag="VIS73_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5762583.1"
                     /translation="MTGPLLEQVKARWRKLSLRFGRRESIFWALLATIALALAVPGGI
                     FSWRLIADERAGVEQAFAAELKRKTELFALTVTEPLWNFDKAAAERLAETFLRDKRVV
                     GLQVRDASLGDFLSIERQARISRQVHRQEAEVTRNGKRIGRIEVAIDDGADFAVVEEL
                     RAWLLNAMVVQFIFAIGLIALTLKYRVSDRLRRLESFSDQLAEGHLEAQLPDTRADEI
                     GRLQHHLDQMRRGWQQALASRDEATVRVTGLLAEQQAMLDNAVVGVAFIEKRTFKAVN
                     AYLEKLLGYGEDEIVGKSTRLLYSTEAIYNEIGRNGYKILASGDTYSIDALLSRKDGS
                     LFWARLSGHALRKGDPQGGSIWLIEDISARKEAEEQLRLAGLVFESSAEGIVLTDADA
                     RILSVNPAFERITGYSAAEAIGERPSIMQSGRQNDGYYQEMWQALREKGLWAGEIWNQ
                     RKDGTQYPEWLTISAARDARGQVTHYVGVFTDITDLKRSEEQLRHMAHHDPLTDLANR
                     ALLDDRLDHALRRAHRENRLLAVLFIDLDRFKTVNDTLGHDVGDELLIEIAKRLQASL
                     RDGDTLARLGGDEFVLILEDLESPRMAGRVAEKIRSVLNEPVYVSGHELFVAGSIGIS
                     LYPDDGDDVATLLKAADSAMYAAKGAGRNGYCFYTAQMTESALERLQIEQQLRHALDS
                     ERFVLYFQPQFSLGNGRLIGAEALIRWEHPTLGVVPPDRFIPAAEESGLIVPIGEWVL
                     RESCRQLSAWRRAGVAIPKVAVNVSPRQLGRGDLPSLVRRVLAETGLPAADIELEVTE
                     SVAALGESLLAELNELHSLGVKLAIDDFGTGFSSLAYLGRMPIDILKIDRVFVQDVDI
                     NSANASIIRAVVELARGLRVSVLAEGVETEDQRALLKSFGCHSVQGFLLGKPLAPDDF
                     AARFGKAGENTTKKADATAVRPVGTNCS"
BASE COUNT          848 a         1478 c         1360 g          739 t
ORIGIN      
        1 acgaaccaat tgggatcttc gccttctcga tgaagcgctt ggcccgcagc agcagaaagc
       61 ccgagggctt gccgtcgcgt acgacgtagt agggcggtcg atcgagcacg atgatctgga
      121 tcggttcggc gtcgctccgc gtcggcgccg ggttggcgcc ggccaggccg ggcgcgaggg
      181 ccagcgcagc caccagcagt cggcaggcga tcatgcgtcg cggcaaaccg cgctcatccg
      241 gttcagggca ggacccgcat gattgccgta tccattcgct ccatcagttc gtcgctgacc
      301 tgccggctgc acaggatgtg ccggcgcagg ccgggcggca tgtcgggaaa gctcagggcc
      361 agcaattcct ccttggggaa atcgcgggcg ctggcgcgca ggtagtcgaa atcatcctga
      421 tcgatgaaca tcaggtcggc gcggccggcg gcgacattct gcgcgacctg aagaggcgcc
      481 gtcagcatgc gctcgacgcg cccggcaaag gacgcgatca gcgcgtcgag atccggcccg
      541 taggaaacgc cgtcggccgc agccagcgac agactgcggt cgccggcaat cgccgccagc
      601 gaaccggcag cgcgcaggcg gtcggcgagc tgccgccgtg ccagcaccag atgcgggcga
      661 tcctgatgga tcggccgcgt gaacttcgca aatgcctcgc gcgacggaat acggtaccag
      721 ccaaacgagc agaccgggcc gtgattgctt tcgatctcgg cgaacagacg tttcggcggc
      781 agcatctcgt agctcgcccg aacgccggcg aggtcgagaa tcttgcgcgt ccgctcgtag
      841 agaaagccct tcggctcgcc cttctcgaga tacgaatagg gaggcttgtc gcggaacagc
      901 atgcgcaact ggacctcggc agcgccagcc cgcaggctca cggcaccgag cagtagaacg
      961 aggctgccgg cggcgatgcg cagcgaacgg cgtcgccgca gcgtccagat catgttgtaa
     1021 tgccctctcc caagcagtgt tgaatcctat gacaggcccg cttctcgaac aagtgaaggc
     1081 gcgttggcgc aagctgtcgc tgcgctttgg ccggcgcgag tcgatcttct gggccctcct
     1141 ggcaacgatc gcgctagccc tcgctgtgcc gggcggtatc ttcagctggc gcctgatcgc
     1201 cgacgagcgc gccggcgtcg aacaggcgtt tgccgctgaa ctcaagcgca agaccgagct
     1261 gttcgcgctg accgtcaccg aaccactttg gaatttcgac aaggctgcag ccgaacgtct
     1321 cgccgaaact ttcctgcgcg acaaacgggt ggtcggcctg caggttcgcg atgcctctct
     1381 gggcgacttc ctgagcatcg aacgccaagc ccgcatctcc cgccaggtcc accggcagga
     1441 agccgaagtc acgcgcaacg gtaagcgcat cggccgcatc gaggtcgcaa tcgacgacgg
     1501 cgccgacttc gccgtagtcg aggaactccg tgcctggctg ctcaacgcca tggtcgtgca
     1561 gttcatcttc gccatcggcc tgatcgcatt gacgcttaag taccgggtgt ccgaccggct
     1621 acgccggctc gaaagctttt ccgaccagct tgccgaaggc cacctcgaag cacagctgcc
     1681 cgatacccgc gccgacgaga tcggccggct gcagcaccac ctcgaccaga tgcgccgcgg
     1741 ctggcagcag gcgctagcct cgcgcgacga ggcgaccgtg cgagtcaccg gtctgctcgc
     1801 cgagcaacag gcgatgctcg acaacgccgt ggtcggcgtc gccttcatcg aaaagcgaac
     1861 cttcaaggcc gtcaacgcct acctcgagaa attgcttggc tacggcgaag acgaaatcgt
     1921 cggcaagtcc acccgcctgc tctattcgac cgaagccatc tacaacgaga tcgggcgcaa
     1981 cggatacaag atcctcgcca gcggcgacac ctattcgatc gacgccctgc tcagccgcaa
     2041 ggacggctcg ctgttctggg cccgactgtc tgggcatgcg ctgcgcaagg gcgacccgca
     2101 gggcggcagc atctggctga tcgaggacat cagcgcccgc aaggaagccg aggaacagct
     2161 tcgccttgca gggctcgtgt tcgagagcag cgccgaggga attgtcctga ccgatgccga
     2221 tgcccggatc ctgtcggtaa acccggcgtt cgaacggatc accggctaca gcgccgccga
     2281 ggcaatcggc gaacgcccgt cgatcatgca gtcgggccgg caaaacgatg gctattacca
     2341 ggaaatgtgg caggcgctgc gcgagaaggg actgtgggcc ggggaaatct ggaaccagcg
     2401 caaggacggc acgcagtatc ccgaatggct gaccatcagc gccgcccgcg acgcccgcgg
     2461 tcaggtcacc cactatgtag gcgtcttcac cgacatcacc gacctcaagc gctccgagga
     2521 gcagttgcgc cacatggcgc accacgatcc gctgaccgac ctcgccaacc gcgccctgct
     2581 cgatgaccgc ctcgatcacg cgctgcgccg cgcgcaccgc gagaatcgtc tgctagcagt
     2641 gctgttcatc gacctcgacc gcttcaagac cgtcaacgac acgctcggcc atgacgtcgg
     2701 tgacgagctg ctgatcgaga tcgccaagcg gctgcaggcc agcctgcgcg acggcgacac
     2761 gctcgcccgg ctcggcggcg acgaattcgt gctgatcctc gaagatctcg agtcgccgcg
     2821 gatggccggc cgcgtcgccg aaaagattcg ctcggtgctt aacgaaccgg tctacgtctc
     2881 cgggcacgaa ctgttcgttg ccgggagcat cggcatcagc ctctaccccg acgatggcga
     2941 cgacgtagca acgctgctca aggccgccga ctcggcgatg tatgctgcca agggcgccgg
     3001 ccgcaacggc tactgcttct acaccgcgca gatgaccgaa agcgccctcg agcgactgca
     3061 gatcgagcag cagttacgcc acgccctcga cagcgaacgc ttcgtcctct acttccagcc
     3121 ccagttctcg ctcggcaacg gtcgcctgat cggcgccgaa gcgctgatcc gctgggagca
     3181 cccgacgctg ggcgtcgtcc cccccgaccg cttcattccg gcggccgagg aatccggtct
     3241 aatcgtccca atcggcgaat gggtgttgcg cgagtcgtgt cggcagttgt cggcgtggcg
     3301 gcgcgccggc gtcgcgattc cgaaagtcgc ggtcaacgtt tcgccgcggc agctcggtcg
     3361 cggcgacctg ccatcgctgg ttcgccgggt cctcgccgaa accggactgc ccgccgccga
     3421 cattgagctt gaagtcaccg aatcggttgc cgcactcggc gaatcgctgc tcgccgagct
     3481 caatgaactc cactcgctcg gcgtcaagct ggcgatcgac gacttcggca ccggcttctc
     3541 ctcgctcgcc tacctcggac ggatgccgat cgacattctc aagatcgacc gcgtcttcgt
     3601 ccaggatgtc gacatcaaca gcgccaacgc ctcgatcatc cgcgccgttg tcgaactcgc
     3661 ccgcggcctg cgcgtcagcg ttcttgccga aggcgtcgaa accgaagacc agcgcgcgct
     3721 gctcaaatcc ttcggctgcc attcagtaca gggattcctg ctcggcaagc cactggcacc
     3781 agacgacttc gcggcgcgat tcggcaaggc cggcgaaaac acaacaaaaa aggccgacgc
     3841 aacggccgtg cgaccggtag gaacaaattg cagttgacgt gaagcaacta gttgcagaag
     3901 ttgactctct tctgcgacga aacgagcacc taagcgaccc aaggcagaag caactctctg
     3961 cacttagtaa ttctctcgcg gacctcgttg cagtaagtca ccctccgctg cgggcccgga
     4021 tttaccgccc tactgccagg ctagaagcgc ctaacacctg gtgtagtcaa ttggcggccg
     4081 gccgctgctc ggccaatgcc gcccttgtgg atcaggccgg aatctggcgg ctctcgagtc
     4141 gaaccgggca ttcataggac aactaagggg atagaccgaa ggtcagcagc gggccggatg
     4201 tttcctcctg ccatatgact gatcaactgc acgactcttg gcagtcaggt gcggctgttc
     4261 ttttcttgtt gggcaactga cctgagattt cgcgccatgc cgtttgagct gcctcggcag
     4321 gccctttagg gactccagac tcgctaaatg cgatacaagc acttcggtca ctaggagcgt
     4381 caaatcaacc ctgatgaggc gccactgcga agtatcgcag ggcgt
//
LOCUS       DATDAA010000160         9161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_466720,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000160 DATDAA010000000
VERSION     DATDAA010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466720"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VIS73_05215"
     CDS             complement(<1..399)
                     /locus_tag="VIS73_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEY5762584.1"
                     /translation="MPKVTLPDGSVRSFDAPVTVAEVAASIGAGLARAALAGKVDGRM
                     VDTSYRIEADAALAIVTDRDPDGLEIIRHSTAHLLAYAVKELFPEAQVTIGPVVENGF
                     YYDFAYKRPFTPEDLAAIEKRMGELAKRDIA"
     gene            640..1155
                     /locus_tag="VIS73_05220"
     CDS             640..1155
                     /locus_tag="VIS73_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY5762585.1"
                     /translation="MNRLIARLFVPLLLLFAAGVSPLGATPGEVPVGATLRNVPMQGL
                     TGDSRFLADYQGKPLLINVWASYCPPCLAEMGSLERLYRSRRGKLEVIGISIDDYRDR
                     AELFLSKAGTSFAHYIDYQLTLENMLGGNRIPLTVIVGADGRVRQKVFGAREWDSAES
                     QALIDAALAAK"
     gene            1168..1785
                     /locus_tag="VIS73_05225"
     CDS             1168..1785
                     /locus_tag="VIS73_05225"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY5762586.1"
                     /translation="MSIDWADVLRVAATLFFVMDPIGNIPLFNVLLKQYEPRRRAVII
                     ARELCFALLILLSFLYAGNVILSFLGLTQPTLNLAGGILLFLIALRMVFPDAGSHDDA
                     PVDDPFIVPLAMPMVAGPSSIAILLLTSSSHPEQMLDWTVALLLAWTVTTIILVVSPM
                     ILKLLGKRGLRALERLMGMLLVLLAVQMFLNGLTQYLQHVAGAGA"
     gene            1844..2476
                     /locus_tag="VIS73_05230"
     CDS             1844..2476
                     /locus_tag="VIS73_05230"
                     /inference="COORDINATES: protein motif:HMM:NF008320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YchE family NAAT transporter"
                     /protein_id="HEY5762587.1"
                     /translation="MLEFTAYTKTLIGLLAVLNPLGAVPIFVAMTPGASDDQRREIAR
                     VVVIAVTLILLAALVFGEVLLDFFGISINSFRVGGGILILLMAISMLQAKVSGTVHTE
                     AEKEDSGSKSSVAVVPLATPLLAGPGAISTVILYADRGSGAIHYTLIAVDILLLCALL
                     WLVFKALPWISRHISQTGINIFTRIMGLILAALSIEFIASGLKGLFPALA"
     gene            complement(2550..2626)
                     /locus_tag="VIS73_05235"
     tRNA            complement(2550..2626)
                     /locus_tag="VIS73_05235"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2590..2592),aa:Val,seq:gac)
     gene            2964..3674
                     /locus_tag="VIS73_05240"
     CDS             2964..3674
                     /locus_tag="VIS73_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY5762588.1"
                     /translation="MKISKYWRLDPAVLDRGFSAFDSPRLAEPADFVARVRPGEGIVL
                     AGWVEREELGRVTALGVCLDRNDDGLLVDWRESDARLRPNAAARANWRGKSSFVFADA
                     VAVRYGLADLFGERFPDLGEDAVTTHTLSPGVHRRLPNNAVPGYVFLIRSLHGFKIGK
                     TANIQNRVRLFGMKCPFPISLEHYAWFEDCSGAERALHHRFHAKRLEGEWFDLAESDI
                     AYIKSLGKEAQLKVLAAA"
     gene            complement(3747..4367)
                     /locus_tag="VIS73_05245"
     CDS             complement(3747..4367)
                     /locus_tag="VIS73_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEY5762589.1"
                     /translation="MISNADKRLPLLWAGALVLVAALIVSGIAPYDRLTWLMEVLPVM
                     IVLPVLALSHRRFPLTTLLYVAIFAHALVLIGGGAYTYARVPFGFWLQEAWDLSRNPY
                     DKIGHFMQGFVPALAAREILIRGQWVRGPRMLAFLVVCVVLAISASYELIEWAAALML
                     GQGAEEFLGTQGDPWDTQSDMFMALTGAMIALLLLSKWQDRQIREL"
     gene            complement(4364..4855)
                     /locus_tag="VIS73_05250"
     CDS             complement(4364..4855)
                     /locus_tag="VIS73_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY5762590.1"
                     /translation="MATHRAEIIWERGVQPFSDNRYSRRHLWRFDGGAEVVASSSPSV
                     VPLPYSLAEAVDPEEAFVASLSSCHMLWFLSLAAKAGLLVDGYRDHAEGRMEKDAAGR
                     DWIAVVTLRPAVRFSGARKPPPAEILALHHDAHAACFIANSVRSEVRCEPQTDLSYNE
                     KWR"
     gene            complement(4908..5153)
                     /locus_tag="VIS73_05255"
     CDS             complement(4908..5153)
                     /locus_tag="VIS73_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762591.1"
                     /translation="MHCFVERRAPLAAELQGLRQSMRADAAIWISWPKKTSGVVSDVS
                     EDVIREIALPLGLVDIKVCAVSPVWSGLKLVIRRELR"
     gene            complement(5367..5675)
                     /locus_tag="VIS73_05260"
     CDS             complement(5367..5675)
                     /locus_tag="VIS73_05260"
                     /inference="COORDINATES: protein motif:HMM:NF018599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEY5762592.1"
                     /translation="MLELRPSCEHCNKPLPPASTEARICSYECTFCATCVETLLHNVC
                     PNCGGAFAPRPVRPATEWKPGAGLARDPASTVVKHRPVDLERHERFAARIRELAPEQR
                     "
     gene            complement(5663..6190)
                     /locus_tag="VIS73_05265"
     CDS             complement(5663..6190)
                     /locus_tag="VIS73_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762593.1"
                     /translation="MADVCPDPDDESALIAVVDAAFAVVDRPTHFTDFTHCCEYAEND
                     ALLRARDRGTLRLDDVGNPGCDPLCFCSAQGLAWYFPALARLALAECGPGAAWYGEQL
                     QFQLSYGGPDNRLLAYCDDGQRDAVARLLGHLVATRAALIDDYGMADDFLRCHALWSD
                     SDLLAKPAGDTPCSN"
     gene            6283..7680
                     /locus_tag="VIS73_05270"
     CDS             6283..7680
                     /locus_tag="VIS73_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HEY5762594.1"
                     /translation="MPYESSLGVTRHRFADLRALLAAASPLRSGDVLAGIAAANAEQR
                     MAARLCLAELSLATFLDEALVPYEDDEVTRLIVDSHERDAFAEIAHLTVGDFRDWLLG
                     DGADSETLSRVAPGITPEMAAAVSKLMRNQDLIIVARKCRVVTAFRDTIGLPGRLAVR
                     LQPNHPTDDPRGIAASIYDGLLYGCGDAVIGVNPVSDSLPAFHRLWQLLDELITHYDI
                     PTQSCVLTHATNQIRAIEQGAPVDLVFQSIAGTEAANRSFGIDLALLAEARDAALSLK
                     RGTVADNVMYFETGQGSALSANAHHGIDQQTCEARAYAVARAFKPLLVNTVVGFIGPE
                     YLYDGKQIIRAGLEDHFCGKLMGVPLGCDICYTNHAEADQDDMDTLLTLLGTAGVSYV
                     MGVPGADDVMLNYQSTSFHDALYVRNLLGLRHAPEFEAWLQRMQVLDDRGRLVGQAPP
                     AMLPGIESLAGEAEP"
     gene            7677..8459
                     /gene="eutC"
                     /locus_tag="VIS73_05275"
     CDS             7677..8459
                     /gene="eutC"
                     /locus_tag="VIS73_05275"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003971.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HEY5762595.1"
                     /translation="MSRSRSIRPDPWRALRTLTPARIALGRVGSALPTAEVLRFGVAH
                     AQARDAVHEAFDCDRLARDLQTLDRTPLHVHSRAPDRATFLLRPDLGRRLRTEDADSL
                     RRHARAGGLCLVLGDGLSARAVHAHAPPLIAEFLRLVADRFAPVTTVIAEQARVALGD
                     EIGACLGSAIVAVLIGERPGLSSPDSLGIYLTFAPHVGRNDAERNCLSNIRAEGMPPA
                     KAARRLLWLVDAASRLQLTGVGLKDDSDAAHQTRASLPGCRD"
     gene            complement(8469..8867)
                     /locus_tag="VIS73_05280"
     CDS             complement(8469..8867)
                     /locus_tag="VIS73_05280"
                     /inference="COORDINATES: protein motif:HMM:NF036460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5329 family protein"
                     /protein_id="HEY5762596.1"
                     /translation="MIALRGTLRIVAAGALIFSCALPFPANATAVEREIELLLGRLAA
                     SGCEFERNATWHAAEAVRTHLLRKRDALARRGAIESSEQFVELAGSGSNTSGKPYRVR
                     CGGSAPVDSRIWLLEQRRLLRQTGGAAKGS"
     gene            complement(8864..>9161)
                     /locus_tag="VIS73_05285"
     CDS             complement(8864..>9161)
                     /locus_tag="VIS73_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762597.1"
                     /translation="APIEPGMPVPPVLVVAPPPITEPRGAIAPKFAGGEHKCVGLAAA
                     LQAVCAEMDCHYFDAGTRIHASPIDGVHLDADQHAVLGRELAPVVGALLGGKSA"
BASE COUNT         1564 a         3257 c         2824 g         1516 t
ORIGIN      
        1 cgcgatgtcg cgctttgcca gctcgcccat gcgcttttcg atcgccgcga ggtcctcggg
       61 cgtaaacggg cgcttgtagg caaagtcgta gtagaagccg ttttcgacga ccgggccgat
      121 ggtcacctgc gcctcgggga acagctcctt gaccgcataa gccagcaggt gcgcggtgga
      181 atggcggatg atttcgaggc cgtcggggtc tcgatcggtg acgatcgcca gcgcggcgtc
      241 ggcctcgatg cgatacgagg tatcgaccat cctgccgtcc accttcccgg ccagcgccgc
      301 acgcgcgagc cctgcaccga tcgacgcggc gacttcggct accgttaccg gggcgtcaaa
      361 ggaacgaacc gagccatcgg gcaaggtgac tttcggcatt tcaactcccg ccgcatcgcc
      421 agcggcacgc aaaaaaaaaa gcgcggcagg cgccgcagct cgattccact gaatcgccgg
      481 cccgtcgtgc cgccggcgcc ccaggcttca ggcacgcgac gaaccccgcc gtccggcaca
      541 aattcagggc gagaattata gattcccgtg ccgggcctgt ccatgaagtg cggagcccgt
      601 gaaacgccgc ctgcggcccc gcattgatac actcgcgcca tgaaccgcct gatcgcccgc
      661 ctgtttgtcc cgctgctgct cctgtttgcc gccggtgtct ccccgctggg ggctacgccg
      721 ggcgaggtcc cggttggcgc gaccctgcgc aacgtaccga tgcaggggct gaccggcgac
      781 tcgcgcttcc tcgccgacta ccaagggaaa ccgctgctga tcaacgtctg ggccagctat
      841 tgcccgccct gcctggccga gatgggttcg ctcgagcgcc tctaccgttc gcgccgcggc
      901 aagctcgaag tcatcggcat ctcgatcgac gactaccgtg accgcgccga actgttcctc
      961 tcgaaggccg gaacgagctt cgcgcactac atcgattacc agctgacgct ggaaaacatg
     1021 ctcggcggca accgtatccc gctgaccgtt atcgtcgggg cggacggacg cgttcggcag
     1081 aaggtcttcg gcgcccgcga gtgggacagc gccgaatcgc aggcgctgat cgacgcggca
     1141 ctcgccgcaa agtagcggag catggccttg tccatcgatt gggccgacgt gcttcgcgtc
     1201 gccgcaacgc tcttcttcgt catggaccca atcggcaaca tcccgctgtt caacgtactg
     1261 cttaagcagt acgagccgcg gcggcgcgcc gtcatcatcg cccgcgaact ctgcttcgcg
     1321 ctgctgatct tgctgtcctt cctttacgcc ggcaacgtga tcctcagctt tctcggcctg
     1381 acccagccga cgctcaatct ggccggtgga atcctgctgt tcctgatcgc gctgcgcatg
     1441 gtctttcccg acgccggcag ccacgacgac gcaccggtcg acgacccgtt catcgtgccg
     1501 ctggcaatgc ccatggtcgc cggtccctca tcgatcgcca tcctgctgct gacgagttcg
     1561 tcgcacccgg agcagatgct cgactggacc gtcgcactgc tgctcgcctg gaccgtgacg
     1621 accatcatcc tcgtcgtctc gccgatgatc ctcaagctgc tcggcaagcg cggactgcgc
     1681 gcgctcgaaa ggctgatggg aatgctgctc gtgctgctcg ccgtgcagat gttcctcaac
     1741 ggcctcaccc aatacctgca gcacgtcgcg ggcgccggcg cctgaccggc gcccaggcag
     1801 accctcccga cccgaccggc gccacccctg gaaatccaac accatgctcg aattcaccgc
     1861 ttacaccaag accctgatcg gcctgctcgc cgtgctcaat ccgctgggtg ccgtgccgat
     1921 cttcgtcgcc atgacgcccg gcgccagcga cgaccagcgt cgcgagatcg cccgcgtggt
     1981 ggtcatcgcg gtgacgctga tcctgcttgc tgcgctggtc tttggcgaag tcctgctcga
     2041 cttcttcggc atcagcatca attccttccg cgtcggcggc ggcatcctca ttctgctgat
     2101 ggcgatctcg atgctgcagg ccaaggtcag cggcacagtc cataccgagg ctgagaaaga
     2161 ggacagcgga tcgaagtcct cggtcgccgt cgttcctctg gcaacgccgc tgctagccgg
     2221 cccgggggcg atcagcacgg tgattctcta cgccgaccgc ggcagcggcg cgatccacta
     2281 cacgctgatc gccgtcgaca tcctgctgct ctgcgcgctg ctctggctgg tcttcaaggc
     2341 cctgccgtgg atctcgcggc acatcagcca aaccggcatc aatatcttca cgcgcataat
     2401 gggtctgatt ctcgccgcgc tgtcgatcga attcattgcc agcggcctca aggggctgtt
     2461 cccggcgctg gcctgaacgg caggcaaaga aaaagcgggg aaccgattcc tcgatttccc
     2521 gctttctgat cctgccccta ccaattcgtt ggtaggcgcg attggactcg aaccaacgac
     2581 ccccaccatg tcaaggtggt gctctaacca gctgagctac gcgcctacag aagagcgcgc
     2641 attctagtca gctcgccagg aaagtcaaga ggctcttccg agagtgccgc cgtgcggccg
     2701 tttccgcacg atcactccaa tcagattgcg cccgcctgcc gcattgggcg acccgctcgt
     2761 tgatacacgc tccgggtctt cgagttcaga tcacgctcct tgttggtgcg gctgccgaac
     2821 tcgctcccgt gacaaattcc atagcgagct cgggttagct acggactccc atagcctccc
     2881 ctgccacact cagaactcga tgagaccggg tcgttgaaac gccgcgacgc cgacggataa
     2941 atcacaacca aactggagtc gccatgaaga tcagcaagta ctggcgtctt gatcccgcag
     3001 tcctcgacag gggcttctcc gcattcgact cgccccggct cgccgagccc gccgatttcg
     3061 ttgcccgcgt acgaccgggt gagggcatcg tgctggccgg ctgggtcgaa cgcgaggaat
     3121 tgggccgcgt cacagccctt ggcgtctgct tggaccgcaa cgacgacggg ctgctggtcg
     3181 actggcgcga gagcgatgct cggctgcggc cgaatgcggc agcacgggcc aactggcgag
     3241 gcaaatccag cttcgtcttc gccgatgccg tagctgtccg ctatggccta gccgacctgt
     3301 tcggcgagcg cttccccgat ctcggcgagg atgccgtcac gacccacacg ctctcgccgg
     3361 gcgtgcaccg ccgcctgccg aacaacgccg tccctggcta tgtcttcctc attcgctcgc
     3421 tgcatggctt caagatcggc aagacggcga acattcagaa tcgcgtccgc ctgttcggca
     3481 tgaaatgtcc gtttccgatc agcctcgagc actacgcctg gttcgaggac tgcagcggcg
     3541 ccgagcgcgc gctgcatcac cgcttccacg ccaagcggct cgaaggcgag tggttcgatc
     3601 tcgccgaaag cgacatcgcc tacatcaaga gcctcggcaa ggaagcccaa ctgaaggtgc
     3661 tcgctgccgc ctgaggccgg tctgcgcgcg acctgtgatc ggtcgaactc gcgcgaacgg
     3721 ccgtgcctac gcgggccaag gccctgtcac agttctcgga tctgccgatc ctgccatttt
     3781 gacaggagca gcagcgcaat catggcaccg gtcagcgcca tgaacatgtc cgactgggta
     3841 tcccagggat cgccctgcgt gccgaggaac tcctcggcgc cctgcccgag catcagcgcc
     3901 gcagcccact cgatcagctc gtagctcgcg ctgatcgcca gcaccacgca gacgaccaga
     3961 aaagccagca tgcgcggccc gcggacccac tgcccgcgga tcaggatctc gcgcgctgcc
     4021 aacgccggga cgaagccctg catgaaatgc ccgatcttgt cgtagggatt gcgcgacaga
     4081 tcccacgcct cctgcagcca gaatccgaac ggcacgcggg cataggtgta ggcaccaccg
     4141 ccgatcagta ccagcgcgtg cgcgaagatc gccacgtaga gcagcgtcgt cagcggaaag
     4201 cgccgatggc tcagcgccag cacgggcaac acgatcatca ccggcagcac ttccatcagc
     4261 caggtcaggc ggtcgtaagg cgcgatcccg gagacgatca gcgccgcaac cagcacgagc
     4321 gcgccggccc agagcaaggg cagccgctta tcggcattcg aaatcatcgc cacttctcgt
     4381 tatagctcaa gtcagtctgt ggttcgcaac gcacttccga acgtaccgag ttggcgatga
     4441 aacaagcggc atgcgcgtcg tggtgcaggg ccagaatttc cgctggaggg ggctttcgtg
     4501 cccccgagaa ccgcaccgcc ggacgcagcg tcaccacggc aatccagtcg cggcctgccg
     4561 cgtccttttc catgcgaccc tcggcatggt cacggtagcc gtcgacaagc agtccggcct
     4621 tggctgccag cgacaggaac cacagcatgt ggcaactcga cagcgaggcg acgaaggcct
     4681 cttccgggtc gacggcctca gccagcgaat agggcagcgg cacgacgctg ggcgaggacg
     4741 aagccaccac ttcggcgccg ccgtcaaaac gccacaggtg gcgtcgactg tagcgattgt
     4801 cggaaaacgg ctgcacgccg cgctcccaga tgatttccgc acgatgcgtc gccacgtcta
     4861 cgctcgtctt tcccgccagc gaatggcgat caacgctgca ccgggcatca gcgcaactcc
     4921 cggcgaatca ccagcttcag ccccgaccag accgggctga ccgcgcagac cttgatgtcg
     4981 accagcccga gcggcagggc gatttcgcga atgacgtcct cactcacgtc gctgaccacg
     5041 cccgatgtct tctttggcca gctgatccag atcgccgcat cggcccgcat cgattgtcgc
     5101 aggccttgca gctcggcggc caatggggcc cgtcgctcga cgaagcagtg cacgatgtca
     5161 gtccgttccg acaggcgccg ctggaagacc acgcctgccg gcagcggcgc cagccattgc
     5221 cggtactcgg gcggcgcgtg gcgcggcagc agtcgcgtgc cctcgccgat gccgagcttc
     5281 ctggccagcg gcgttcccga ttatccggcg gccggtgcgc tcacgcgcgt ttccgccggt
     5341 acgagtgcgg acagcccagc gcaggcctat cgctgttcgg gtgcaagttc acgaatccgc
     5401 gcggcgaaac gttcgtgacg ttccagatcg accggccgat gcttcaccac cgtactcgcc
     5461 gggtcgcggg ccagccccgc gccaggcttc cactcggtcg ccgggcgcac cggccgcggc
     5521 gcgaaagcgc ctccgcaatt cgggcagacg ttgtgcagca gggtctcgac acaggtggcg
     5581 cagaacgtgc actcgtagga acagatgcgg gcctcggtcg aggctggcgg caacggcttg
     5641 ttgcagtgtt cgcagcttgg cctcagttcg agcatggcgt atccccggca ggtttggcga
     5701 gcagatcgga atctgaccac aaagcatggc agcgcaggaa gtcgtcggcc atgccgtagt
     5761 cgtcgatcag tgccgcacgc gtggcaacca ggtggccaag caggcgggcg acggcgtcgc
     5821 gctgcccgtc atcacaatag gccagcaggc ggttgtcggg gccgccgtag ctcagttgaa
     5881 actgcagctg ttcgccatac cacgcggcac ccggtccgca ttcggcgagc gccagtcgcg
     5941 ccagcgccgg aaagtaccag gcgagccctt gcgcagaaca gaagcacaac gggtcacagc
     6001 ccgggttgcc gacgtcatcg aggcgcagcg tcccacggtc gcgcgcgcgg agcagcgcgt
     6061 cgttctcggc atattcgcag cagtgcgtga aatcggtgaa atgggtcggg cggtcgacga
     6121 cggcgaatgc cgcgtcgact acggcaatca gcgccgattc gtcgtcgggg tcggggcata
     6181 cgtcagccat gccgagatac tggcatatcc gaacgagcct cgcttcgggc acgccgctcc
     6241 agcatcctgc gtctagaatc gacgcaacgc aacggaggcc gcatgcccta cgagtcctcg
     6301 ctcggcgtca cccgccatcg cttcgccgac ctgcgcgcgc tgctggctgc tgccagcccg
     6361 ctgcgatccg gcgacgtact cgccggcatc gccgccgcca atgccgagca gcgcatggcg
     6421 gcccgcctgt gcctcgccga gctgtcgctg gcgaccttcc tcgacgaagc gctcgtgccc
     6481 tacgaggacg acgaagtcac gcgcctgatc gtcgattcgc acgagcgtga cgccttcgcc
     6541 gaaatcgccc atctcacggt tggcgatttc cgcgattggc tactcggcga tggtgccgac
     6601 agcgaaacgc tgtcccgcgt cgcgcccggc atcacgcccg agatggcggc cgcggtgtcg
     6661 aagctgatgc gcaaccagga cctgataatc gtcgcacgca agtgccgcgt cgtcaccgcc
     6721 ttccgcgaca cgatcggatt gcccggacga ctcgcggtgc ggctgcagcc caaccacccc
     6781 accgacgacc cgcgcggcat cgccgcctcg atctacgacg ggctgctcta cggctgcggc
     6841 gacgcggtca ttggcgtcaa cccggtatcg gacagcctgc cggcctttca tcgcctgtgg
     6901 cagctgctcg acgaactgat cacccactac gacatcccga cgcagtcctg cgtgctcacc
     6961 cacgcgacca accagatccg cgccatcgaa cagggcgcgc cagtcgatct ggtattccag
     7021 tcaatcgccg gcaccgaggc cgccaaccgg agcttcggca tcgatctcgc gctgctcgcc
     7081 gaggcacgcg acgccgcgct gtcgctcaag cgcggcaccg tcgccgacaa cgtgatgtat
     7141 ttcgaaaccg gccagggcag cgcgctgtcg gccaacgccc accacggtat cgaccagcag
     7201 acctgcgagg cgcgcgccta cgccgtcgcc cgtgccttca agccgctgct ggtcaacacc
     7261 gtcgtcggct tcatcggccc cgagtatctc tacgacggca agcagatcat ccgcgccggc
     7321 ctcgaagacc acttctgcgg caagctgatg ggggtgccgc tcggctgcga catctgctac
     7381 accaaccacg ccgaggccga ccaggacgac atggacacac tgctgacgct gctcggcacc
     7441 gccggcgtca gctacgtgat gggcgtgccc ggcgccgacg acgtgatgct caactaccag
     7501 agcacctcct tccacgatgc gctgtacgtg cgcaacctgc tcggcctgcg ccacgcgccg
     7561 gagttcgagg cctggctgca gcgcatgcag gtcctcgatg atcgtggtcg cctggtcggg
     7621 caggctccgc cggcgatgct gccgggcatc gaatcgctgg ccggtgaggc agagccatga
     7681 gccgcagtcg atcgatccgc cccgacccct ggcgggcgtt gcgtacgctg acgccggccc
     7741 gtatcgcgct cggtcgcgtc ggctcggcgc tgccgactgc cgaggtcttg cgcttcggcg
     7801 tcgcccacgc gcaggcgcgc gacgccgtgc acgaagcgtt cgactgcgat cgactcgcgc
     7861 gcgatctgca gacgctggac cgcacgccgc tgcatgtcca cagccgagcc cccgaccgcg
     7921 cgacttttct gctgcgcccc gacctgggac ggcgattgcg caccgaggat gccgacagcc
     7981 ttcgccgtca cgccagggcc ggtgggctct gcctggtcct tggcgacggc ctgtcggctc
     8041 gcgccgtgca tgcacacgcg ccaccgctga tcgccgaatt cctgcgcctc gttgccgacc
     8101 gtttcgcacc tgtcaccacc gtaatcgcag aacaggcccg tgtcgcgctc ggtgacgaaa
     8161 tcggtgcatg tctcggcagc gccatcgtcg ccgtgttgat cggcgagcgc ccaggtctct
     8221 cctcgcccga cagcctcggc atctacctca ccttcgcgcc gcacgtcggg cgcaacgatg
     8281 ccgagcgcaa ctgcctgtcg aacatccgcg ccgaaggcat gccccccgcc aaggcagcgc
     8341 gccgattgct gtggctggtg gacgccgcga gccggctgca actgaccggg gtgggactca
     8401 aggacgacag cgatgcggcg caccagaccc gcgcgagcct gcccggctgc cgggactgac
     8461 gggcgcggct agctgccctt ggccgcgccg ccggtctggc gcagcagccg ccgctgctcc
     8521 agcagccaga tgcgactatc gaccggcgcc gagccgccgc agcgcacgcg gtacggcttg
     8581 ccgctggtgt tgctgccgga accggccagt tcgacgaact gttcgctgct ctcgatggcg
     8641 ccgcggcgcg ccagagcgtc gcgcttgcgc agcaggtgcg tccgcaccgc ctcggccgcg
     8701 tgccaggtgg cgttgcgttc gaattcgcag ccggaagcgg caagtcgccc gagcagcaac
     8761 tcaatctcgc gttcgactgc cgttgcattc gccgggaaag gcaacgcgca agagaaaatc
     8821 agcgcccccg ctgcaacaat tcgcaaggtg ccgcgcaacg cgatcacgcc gatttcccgc
     8881 cgagcagcgc accgaccacg ggcgccaact cccggccgag aaccgcgtgc tgatcggcat
     8941 cgaggtgaac cccgtcgatc gggctggcgt ggatacgcgt gccggcgtcg aagtagtgac
     9001 aatccatctc cgcgcatacc gcctgcagcg ccgcggccag ccccacgcac ttgtgttcgc
     9061 caccggcgaa cttcggcgcg atcgcgccgc gcggctcggt gatcggcggc ggcgccacca
     9121 ccagcaccgg cggcaccggc atgcctggct cgatcggcgc g
//
LOCUS       DATDAA010000161         2653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_222085,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000161 DATDAA010000000
VERSION     DATDAA010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222085"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            451..1410
                     /locus_tag="VIS73_05290"
     CDS             451..1410
                     /locus_tag="VIS73_05290"
                     /inference="COORDINATES: protein motif:HMM:NF005292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEY5762598.1"
                     /translation="MIRERLPADIVLAANRIAPHLRETPLDYSAYFSRESGASVYLKL
                     ENLQYTCSFKLRGAFNKLLTLAPEQRQAGCVAASSGNHGAAVAYAMQKLGITGVIFVP
                     EQTSTTKVEAIRLAGAEVRFHGTDGLDTELHAREFADASGMAYLSPYNDIDVVAGQGS
                     CGVEIARQLAPVDAVFVAVGGGGLVSGVGSFLRSVNPAIRVVGCQPEASAVMAESVEA
                     GRILDLPSGPTLSDGTAGGIEPGAITFDICREVVERFVTVSEAEIAEAMRRFIDSHHM
                     LLEGAAGVALAGFLSSAKEFEGRNVVVIICGGNISRETLRKVI"
     gene            1492..>2653
                     /locus_tag="VIS73_05295"
     CDS             1492..>2653
                     /locus_tag="VIS73_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5762599.1"
                     /translation="MKYRIIATLAALSTCGHALADTTAFVNVTVIPMDAAGARPAQTV
                     IVVGDRISAIGDVDSTPVPQAALVVDGTERYLVPGFTEMHGHVPGGNSDSLQRVLTLY
                     IANGVTTVRGMLGQPSHLELRERIRSGEQLGPRLITSGPSFNGRSVAGVEAAVTMVRE
                     QHAAGYDFLKIHPGLSLGEFEAIAATARELGIPFAGHVPEDVGIETALAAGIATIDHL
                     DGYMEGLLPPNEDPSGGLGGFFGLFIADQAQTEKIAAIAAMTAEAGTWNVATESLFAH
                     VTSPDLVTGELAGRPEMRYMPEDSVSRWQAARDEVLADKNYDAGTARRALEIRRQLIR
                     ALRDAGAGLLLGSDSPQIFNVPGFALHHELEFLVEAGLTPFEALRAGTASPAE"
BASE COUNT          470 a          843 c          866 g          474 t
ORIGIN      
        1 cgggttcggg ctcgaggccc agggtctcga aaaaagcgac attccgccgg taagcggtgt
       61 cgaagtcgcc gattatccag gaatagaccg aaccgagaaa atcatcgtag tgtgtcttga
      121 cggattccat gggcgcccga gcctgccgct taaccatgcg cgtactgtga cagaaacgcg
      181 ggacgtatgc gacgcatcgc agcgggctcc gagcttgttg gccgcgcgga tccccgctaa
      241 gctcgccgac actgcccgct gctgggatga tcattcaatg tgcatgcgat tcggttccga
      301 caacggtcgc ggcttccttt cgacacagga tccgccgccc gcacgtccac cgacgcccgc
      361 gtccacggcg gcgctcccgg gttcgaacga gagaccggca cggttacgcg aattcctggc
      421 gggccttctt ttaagacggg gctacggcca gtgatccgcg agcggctgcc cgcagatatt
      481 gtccttgccg ccaaccgaat agcgccgcat ctgcgcgaga ctccgctcga ctactccgcc
      541 tacttcagcc gagagagcgg cgcctcggtg taccttaagc tcgaaaacct tcagtacacc
      601 tgttcgttca agttgcgcgg cgcattcaac aagctcttga cgctcgcgcc ggaacagcgg
      661 caggccggct gtgtcgcagc atcgagcggc aatcatggcg ccgccgtcgc ctacgcaatg
      721 caaaagctcg ggattaccgg ggtcatcttc gttccggagc agacctcgac gaccaaggtt
      781 gaagccattc gactcgccgg cgccgaggtt cgtttccatg gcaccgatgg cctcgacacc
      841 gagttacacg cgcgggaatt cgccgacgcc agcggcatgg cctacctgtc tccttacaac
      901 gatatcgacg tcgtcgcagg gcagggctcc tgcggcgtgg agatcgcccg ccagctggcg
      961 ccggtcgatg ccgtcttcgt cgcggtcggc ggcggcggac tggtgagcgg cgtcggcagc
     1021 ttcctgagat cggtcaaccc ggcgatccgg gtcgtcggct gccagccaga agcgtcggcg
     1081 gtcatggccg aatccgtcga ggccggccgg atcctggacc tgccgagcgg gcccactttg
     1141 tcggatggca cggccggggg aatcgagccg ggcgccatca ccttcgatat ttgccgagag
     1201 gtcgtcgagc gattcgtcac ggtgtccgaa gccgagatcg ccgaggcgat gcggcggttc
     1261 atagacagtc atcacatgct cctcgaaggg gcggcgggcg tcgcgctggc gggattcctg
     1321 tcatcggcga aggaattcga gggtcggaac gttgtcgtga ttatctgcgg cggcaatatc
     1381 agtcgtgaaa cgctgagaaa agtcatctga aggacggggc agggcgccgg acggaaccgc
     1441 tgcgactata ctcactcaaa gggattgatt ccgcagccag ctggcaagtc tatgaaatat
     1501 cgcattatcg caacactcgc cgcgctttct acctgcggtc atgcattggc cgacacgacc
     1561 gccttcgtta acgtcaccgt gatacccatg gacgccgcgg gcgcccgccc ggcgcagacg
     1621 gtgatcgtgg tgggcgatcg gatcagcgcc atcggcgacg tcgacagcac cccggttccg
     1681 caggcggcgc tcgtcgtgga cggcacggag cgctatctcg tccctgggtt caccgaaatg
     1741 cacgggcacg tgcccggagg aaattcggac agcctgcagc gggtgcttac gctttatatt
     1801 gccaacggcg tcaccacggt ccggggcatg ctcggtcagc cgtcgcacct cgagcttcgc
     1861 gagcgtattc gtagcggtga acagcttgga ccgcgcctga tcacctcggg accgtctttc
     1921 aacggccgca gcgtggcggg cgtcgaggcc gcggtgacga tggtgcggga acagcatgcg
     1981 gccggttacg atttcctgaa aatacacccg ggcctgagcc tgggcgagtt cgaagccatc
     2041 gccgcgaccg cgcgtgagct cgggattccc tttgccgggc acgtgcccga ggacgtcggc
     2101 attgaaacgg cgctcgcggc cggcattgcc acgatcgacc acctcgacgg ttacatggag
     2161 ggactgctcc cgccgaatga ggatccttcc ggcggccttg gcggtttctt cggattgttc
     2221 atcgccgacc aggcgcagac cgaaaagatc gcagcgatcg ccgcgatgac agccgaggcc
     2281 ggcacctgga acgtggctac cgagtcgctg ttcgcgcacg tcacctcgcc cgatctggtt
     2341 accggtgagc tggccggccg gccggaaatg cggtacatgc cggaggacag cgtttctcgt
     2401 tggcaggcgg ccagggacga ggtgctggcc gacaagaact acgacgcggg tactgcccgg
     2461 cgggcgctcg aaattcgccg gcaactgatc cgggcgcttc gcgacgccgg ggcgggcctt
     2521 ctgctcggtt cggattcacc gcagatattc aacgtcccgg gtttcgcgct gcatcacgaa
     2581 cttgaattcc tggtcgaggc gggccttacc ccgttcgaag cgctgcgggc cggcaccgcg
     2641 agcccggcgg aat
//
LOCUS       DATDAA010000162         4634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_396914,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000162 DATDAA010000000
VERSION     DATDAA010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396914"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1443
                     /locus_tag="VIS73_05300"
     CDS             64..1443
                     /locus_tag="VIS73_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HEY5762600.1"
                     /translation="MADPQLRQNFRGAMNFLMAKRAAQFTDAEAFEQLRVTAENIRQH
                     SLSRLPELLEKLEASLTRNGIKVHWAETPEDANRIVLSIAREHQGELIVKGKSMVSEE
                     IELNHEMAKHSVDCLESDMGEYIVQLAGERPSHIIMPAIHKTKEEIARLFADRVPGVD
                     YTDSVDELIQIGRRVLREKFRDTKIGLSGVNVAVAETGTLCLVENEGNGRMCTTVPDV
                     HIAVTGIEKVVDTLDQVPPILSLLTRSATGQAVTTYFNWISSPRRSGELDGPREVHLI
                     LLDNGRTQAYADEQLRKTLQCIRCGACMNHCPVYTRIGGLAYGTTYPGPIGKIVSPHM
                     LGLEATHPLVTASTLCGACGEVCPVKIPIPDLLIRLRTESQRPATEPSAMAGSGSGRT
                     PLVALSFRVWSMIYAHPRLYRVFAWAATRFRALAPRNQGAWTQSRSPMLPAKTSFRDR
                     LRERRSNTH"
     gene            1464..2063
                     /locus_tag="VIS73_05305"
     CDS             1464..2063
                     /locus_tag="VIS73_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEY5762601.1"
                     /translation="MQGLLKLSALIDTSNRYLGRFLTWLILVSVAISAGNAIVRKIFN
                     VSSNAFLEIQWYLFSAVFLLGAGYAFLKNAHVRIDFLSHHMSARTRNLIDAVGIVVFL
                     LPLCYLMIDLAWPVVVNAYESGEMSQNAGGLIRWPVYALVPLGFAVLALQGISELIKR
                     IGFIRGVSPDVIGHAETESKDEIGHEIEEHASHANEEIR"
     gene            2060..3832
                     /locus_tag="VIS73_05310"
     CDS             2060..3832
                     /locus_tag="VIS73_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY5762602.1"
                     /translation="MMEFVAENFVPLLFGGLILFLVTGFPVAFSLAATGLFFGFIGME
                     AGLFPPSLFQALPLRIFGIMQNDTLLAIPFFTFMGIILERSGMAEDLLESCGQVFGPL
                     RGGLALAVIFVGALLAATTGVVAAAVISMGLISLPIMLRYGYNRTIATGVITASGTLA
                     QAIPPSLVLIVMADQLGRSVGDMYQGALLPGVMLVGIYALFVVIVAFVKPKWVPPLPE
                     EARIYREPNGTSGQLSLWVMSGLSWGVGAIWFKLHPIIINPMVNREGDTAPLDEMAIL
                     TMTVIGLFALVIALVNKWFKLGLLSVLAERVTFALIPPLVLIFLVLGTIFLGLATPTE
                     GGAMGAIGALAMAIGKRKLSMKLMNEALENTTKLSIFVMFILIGSTVFSFTFNAADGH
                     VFVEHLFDKLPGGQLGFMIFVNILIFILGMFIDFFEIAFIVLPLLAPVAEKLDINLIW
                     FGVIIAMNLQTSFLTPPFGFALFYLRSVAARSDYTDRITGETIPAVQTTQIYKGAIAF
                     IFIQIFMVGMLISFPQLVTGGIDERKVDLDALKAFEAEFGYGGTEEEDSGDSEGDAEP
                     VVPAGAGSADDAAAALRRSLEENK"
     gene            3949..>4634
                     /locus_tag="VIS73_05315"
     CDS             3949..>4634
                     /locus_tag="VIS73_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY5762603.1"
                     /translation="MERRKFLKNAGLAGVLAAGVAPAVHAQQSIRWRLASSFPKALDT
                     IYGGAEVFSETLSAITGGKFQISVHAGGELMPPFGVVDGVQQGTVECAHTAPYYFFGK
                     DDTFAMDCAIPFGMNSRQLTAWMYHGNGLKLFREFYSNYNIVNFPLGNTGAQMGGWYR
                     KEIKTLADVKGMKMRIGGFGGKVLSAIGGVPQNIPGGEIYQALEKGTIDATEWVGPYD
                     VEKLGFHKVAG"
BASE COUNT          863 a         1447 c         1352 g          972 t
ORIGIN      
        1 acccaggcgc agacgctcca ccaccacccg caaccgagct tcaaggtcgc ctcaagcgaa
       61 gcgatcgccg atccacaact gcgccagaat ttccgtggcg cgatgaattt cctgatggcg
      121 aagcgcgccg cgcagttcac cgacgccgag gcgttcgaac aactgcgcgt aaccgccgag
      181 aacatccgcc agcacagcct ctcgcgctta ccagaactcc ttgagaagct tgaagcctcc
      241 ttgacccgca acggcatcaa ggtgcactgg gctgagactc ccgaagacgc caaccgcatc
      301 gttctttcga tcgcccgcga gcatcagggg gagttgatcg tcaagggcaa gtcgatggtc
      361 agcgaggaga tcgaactcaa tcacgagatg gccaagcata gtgtggactg tcttgaatcg
      421 gacatgggtg agtacatcgt ccagctcgcc ggcgagcgcc cgtcgcacat catcatgccg
      481 gcgatacaca agaccaagga agagattgcc cgtttgtttg ccgatcgcgt tccgggtgtc
      541 gactacaccg actcggtcga cgagctgatc cagattggcc gccgggtcct gcgcgagaag
      601 ttccgcgata ccaagatcgg gctctctggc gtcaacgtcg ccgttgccga aaccggaacg
      661 ctgtgtctgg ttgagaacga gggaaacggc cggatgtgca cgaccgtccc cgatgtgcat
      721 attgccgtca caggaatcga aaaggtcgtc gatacgctcg accaggtgcc gcccatcctc
      781 tcgctgctga cgcgatcggc aaccggccag gcggtcacga cctacttcaa ctggatctcc
      841 agcccgcgcc gttccggcga actcgacggc ccacgcgaag ttcacctcat cctgcttgac
      901 aacggtcgaa cccaggccta cgccgacgag cagctgcgca agacgctgca gtgcatccgc
      961 tgcggcgcct gcatgaacca ttgcccggtc tatacgcgaa tcggcgggct tgcctacggc
     1021 accacctatc ccggtccgat cggcaagatc gtctcgccac acatgcttgg tcttgaggcg
     1081 acgcatccgc tggtgacagc ttcgacgctg tgcggtgcct gcggcgaggt ctgtccggtg
     1141 aaaattccga ttccggacct gctgatccgg ctgcgtaccg aaagccagcg accggccacg
     1201 gagccatcgg caatggccgg ttccggaagc ggccgtacgc ctttggtcgc gctctccttc
     1261 cgcgtctggt cgatgattta cgcgcatccg cgcctctatc gtgtctttgc ctgggcggct
     1321 acgcgattcc gtgcactggc accgcgcaac cagggtgctt ggacgcaaag ccgcagcccg
     1381 atgctgccag cgaaaacgag tttccgcgat cgcctgcgcg agcgcagatc aaacacccac
     1441 tgaatcgaca ccggaggaac tctatgcagg gattgttgaa actctcggcc ctgatcgaca
     1501 cgagcaaccg ttatctcggt cgtttcctga cctggctgat tctcgtgtcg gtcgccatca
     1561 gtgcgggcaa cgccatcgtt cgcaagatct tcaacgtcag ttcgaacgcc tttcttgaga
     1621 tccagtggta tctgttctcg gccgtgttcc tgctcggcgc cggctacgcc ttcctcaaga
     1681 acgcgcatgt ccgcatcgac tttctgtcac atcacatgtc ggcaagaacg cgcaacctga
     1741 tcgatgcagt cggaatcgtc gtctttctgc ttccgctctg ctatctgatg atcgaccttg
     1801 cctggccggt cgtcgtcaat gcgtacgaga gcggcgagat gtcgcagaac gccggcggcc
     1861 tgatccgctg gcccgtctat gcgctggtgc cgctcggttt tgccgtgctg gcgctgcagg
     1921 ggatttctga actgatcaag cgtatcggct tcatccgtgg tgtttcgccg gacgtcatcg
     1981 ggcacgcgga aaccgagagc aaggacgaaa tcggtcacga gattgaagag catgcgagtc
     2041 atgccaacga ggagattcgc tgatggagtt cgttgctgag aatttcgtcc ccctgctatt
     2101 tgggggcctg attctgttcc tggtcacggg cttccccgtt gccttttcgc tggccgcgac
     2161 cggcctgttc ttcgggttta tcggcatgga ggccgggctt tttccgcctt cgctgttcca
     2221 ggccctgccg ctgcgcatct tcggcatcat gcagaacgac acgctgctgg cgattccctt
     2281 cttcaccttc atgggaatca ttctcgaacg aagcggcatg gccgaagacc tgctcgaatc
     2341 ctgcggccag gtattcggac cgttgcgcgg cggcctggcg ttggccgtga tcttcgtcgg
     2401 tgcgctgctg gcggcaacca ctggcgtggt tgcagcagct gtcatctcga tgggcctgat
     2461 ctcgctgccg atcatgctcc gctacggcta caaccgcacc atcgcgaccg gcgtgatcac
     2521 cgcttccgga accctggcgc aggcgattcc gccctcgctg gtgctgatcg tgatggccga
     2581 ccagcttggc cgttcggtcg gcgacatgta tcagggcgcg ttgctgccag gcgtcatgct
     2641 ggtcggcatc tacgcgctgt tcgtcgtcat cgtcgccttt gtcaagccga agtgggtccc
     2701 gccgctcccc gaagaggcgc gcatttaccg cgagccaaac ggcacgagcg gccagctttc
     2761 gctctgggtc atgtctgggc tctcctgggg cgtcggcgcg atctggttca agctccatcc
     2821 aatcatcatc aacccgatgg tcaaccgcga gggagatact gcgccgctcg acgaaatggc
     2881 catcctgacg atgaccgtga tcggactttt cgcgctggtg atcgcactgg tcaacaagtg
     2941 gttcaagctg ggactgctgt cggttctcgc cgaacgtgtc acttttgcgt tgatcccgcc
     3001 gctggtgctg atcttcttgg tgctcggcac gatctttctc ggcctggcaa cgccgactga
     3061 gggtggcgca atgggtgcga tcggtgccct agcgatggca atcggcaagc gcaagctcag
     3121 catgaagttg atgaacgagg cgctggagaa cacaaccaag ttgtcgatct tcgtcatgtt
     3181 catcctgatc ggctcgacgg tgttctcatt cacgttcaac gcggcggatg gtcacgtctt
     3241 tgtcgaacac ctcttcgaca agctgcccgg tggccagctc ggattcatga tcttcgtgaa
     3301 catcctgatc ttcattctcg gcatgttcat cgatttcttc gaaatcgcct tcatcgtctt
     3361 gccgctgctg gcgccggtgg ccgaaaagct cgacatcaac ctgatctggt tcggcgtgat
     3421 catcgcgatg aacctgcaga catctttcct gactccgccc ttcggatttg cgctgttcta
     3481 ccttcgcagc gtcgccgcgc gttccgatta caccgaccgg attaccggtg agaccatccc
     3541 tgcggtacag acgacgcaga tctacaaggg cgcgatcgcc ttcatcttca tccagatctt
     3601 catggtcggc atgctgatca gcttcccgca attggtgact ggcggcattg acgagaggaa
     3661 ggttgacctt gacgcattga aggccttcga ggccgaattc ggctatggcg gaactgaaga
     3721 agaggactcc ggcgactccg aaggtgatgc tgagcctgtg gttcccgcgg gcgcgggcag
     3781 cgctgacgat gcagcagcgg ccttgcgtcg ttcgctggag gaaaacaaat agatcgattg
     3841 aacgaagcgg ctttggccac ttccaatcac atcagattga ccggtgttgt atccgcgaac
     3901 ccgctcatgg gacgcaagtt tttaccacca caaaacgagg agtagacaat ggaaagacgg
     3961 aagtttctaa aaaatgcagg cctcgccggc gtactcgccg ccggtgtggc gcctgccgtc
     4021 cacgcgcagc agtcgatccg ctggcgtctc gcgtccagct tccccaaggc gctggatacc
     4081 atctacggcg gtgccgaggt cttctccgag accctcagtg cgattaccgg cggcaagttc
     4141 caaatctcgg tccacgccgg tggcgaactg atgccgccct ttggcgttgt tgacggcgtc
     4201 cagcagggta ctgtcgaatg cgcccacacc gcgccgtact acttcttcgg caaggacgac
     4261 acctttgcta tggattgcgc gatccccttc ggcatgaact cgcgccagct gaccgcgtgg
     4321 atgtaccacg gcaacggcct caagctgttc cgcgagttct actccaacta caacatcgtc
     4381 aacttcccgc tcggcaacac cggggcgcag atgggcggct ggtaccgcaa ggaaatcaag
     4441 acgctggctg acgtcaaggg catgaagatg cggatcggcg gcttcggcgg caaggtcctg
     4501 tcggcgatcg gcggcgttcc ccagaacatc cccggcggcg agatctacca ggctctggaa
     4561 aagggcacca tcgacgccac cgagtgggtc ggtccctacg acgtcgaaaa gctcggcttc
     4621 cacaaggttg ccgg
//
LOCUS       DATDAA010000163        15272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_396976,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000163 DATDAA010000000
VERSION     DATDAA010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 15272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15272
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396976"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VIS73_05320"
     CDS             <1..395
                     /locus_tag="VIS73_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY5762604.1"
                     /translation="PQPRLTLGLAVRGLATAAIDVSDGLLGDLSHILERSALAAEIAI
                     DDLPPLPAGVDASLARRCQLAGGDDYELVFSAPPTAGLAIAALGRRLGLPLTRFGRVV
                     AGSAGTITLRDPDGQVVDFAERGHDHFA"
     gene            379..903
                     /locus_tag="VIS73_05325"
     CDS             379..903
                     /locus_tag="VIS73_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEY5762605.1"
                     /translation="MTTSPDIRFVFSSPFHLIACGFGSGLSPVASGTVGTLFAWASFP
                     LLQGLFGDVELLVFLVLAFLFGILACHRTGRALGVVDHGVIVWDEIVPFWLVLLLTPE
                     GWLWQTAAFFLFRFFDIVKLPPAGYFDREVKNGFGVMADDLFAALHTLLVIAAAKLWM
                     PFWLPLVQPFLPPL"
     gene            903..1409
                     /locus_tag="VIS73_05330"
     CDS             903..1409
                     /locus_tag="VIS73_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY5762606.1"
                     /translation="MFDQDALETLASAVGQALLARGERLATAESCTGGWLAQAITAIP
                     GSSDWFDRGFVTYANAAKVEMLGVDPATLDRHGAVSEACAREMLAGTLKLSQADWAVS
                     ITGIAGPGGGCFDKPVGSVWFAFGGPAAVADATLQRFEGDRRAVRAQAVAFALETLRG
                     KLATPMRA"
     gene            1551..2573
                     /gene="recA"
                     /locus_tag="VIS73_05335"
     CDS             1551..2573
                     /gene="recA"
                     /locus_tag="VIS73_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY5762607.1"
                     /translation="MEENKSKALAAALAQIEKQFGKGSIMKMGDGDIGEPIDVVSTGS
                     LGLDIALGVGGLPRGRVVEIYGPESSGKTTLTLQVVAEMQKLGGTAAFIDAEHALDPG
                     YAQKLGVKVSDLLISQPDTGEQALEIADMLVRSGSVDVIVIDSVAALVPRAEIEGEMG
                     DSLPGLHARLMSQALRKLTANIKKTHTLVIFINQIRMKIGVMFGSPETTTGGNALKFY
                     ASVRLDIRRIGSIKRGDEVVGNETRVKVVKNKVSPPFREALFDILYGEGISREGEIIE
                     LGVAHKILDKSGAWYAYNGDKIGQGKDNVREFLRGNPDMAREIEAKIRAAVGVHTAAK
                     PAEAEA"
     gene            2585..3016
                     /gene="recX"
                     /locus_tag="VIS73_05340"
     CDS             2585..3016
                     /gene="recX"
                     /locus_tag="VIS73_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEY5762608.1"
                     /translation="MRNQALRLLSRREHSRAELAAKLQATAGEGEDIDGLLDSLESSG
                     LLSDARYAEGRARVRGGGLGDQRLAHELRQRGVAAELIESTLSALPDEADRARAAWQR
                     RFGTRPLDRADWAKQARFLQSRGFAVGTIRRVLDSPDEDEP"
     gene            3020..3754
                     /gene="lptB"
                     /locus_tag="VIS73_05345"
     CDS             3020..3754
                     /gene="lptB"
                     /locus_tag="VIS73_05345"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY5762609.1"
                     /translation="MPTERSTLSATQLKKRYKSRTVVHDVSFEVASGEVVGLLGPNGA
                     GKTTCFYMVVGLIAADGGEVRLDEHSLTHLPIHRRARLGLSYLPQEASVFRKLTVTEN
                     ILAVLELQGLGREATEARLESLLEELHIAHIRNTNAAALSGGERRRVEIARALATEPR
                     FILLDEPFAGVDPIAVLDIQKIIRFLKGRGIGVLITDHNVRETLGICDRACIINAGEV
                     LAAGRPDEIIYDEDVRKVYLGENFRL"
     gene            3797..5272
                     /locus_tag="VIS73_05350"
     CDS             3797..5272
                     /locus_tag="VIS73_05350"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY5762610.1"
                     /translation="MKPALQLKLSQHLALTPQLQQSIKLLQLSTLELNQELERYLLEN
                     PLLERDEEETAAEAPRDADRSNGEAASSATSDESAGERGSEGGDSGEAAPAADDTPTD
                     FGDEWLDESGSYGSKSGRQDDDEDFDPQDAQSSSTSLREHLDWQVALTPLSDRDRTLV
                     RVLIEALDDDGYLTQALEELAESLPEEAEVGVDELLVALRILQSLDPTGVGARNAREC
                     LSLQLKAREPGTIRDLALNLVANHLDQMATRDFAKIKKGLGCDDDQLRSTMAMIRGLD
                     PRPGAKYAPMDTRYITPDVIVRKVRGKWVASVNGDAFPRLRVNRLYAEILSRQRGSGL
                     AGQLQEARWLIKNVQQRFDTIQRVTQAIVDRQRQFFEHGEVAMRPLVLREIADILGLH
                     ESTISRVTSQKYMATPRGIFELKYFFGSHVATEAGGACSATAIRALIKQMVAAENPQK
                     PLSDSQISDILGQQGIVVARRTIAKYREALSIPPTNLRKSL"
     gene            5290..5613
                     /gene="raiA"
                     /locus_tag="VIS73_05355"
     CDS             5290..5613
                     /gene="raiA"
                     /locus_tag="VIS73_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY5762611.1"
                     /translation="MNLHITGHHLEVTPALRDYVTTKLEKVTRHFDHVIDVSVTLSVE
                     KLKQKAEVTLHVRGKDIFVESDDSDLYAAIDALVDKLDRQVLKYKQKLQDHHRGDRIT
                     HHIAE"
     gene            5678..6124
                     /gene="ptsN"
                     /locus_tag="VIS73_05360"
     CDS             5678..6124
                     /gene="ptsN"
                     /locus_tag="VIS73_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEY5762612.1"
                     /translation="MSLLASLLPENHVLLDVEITSKKRLFEYIGLLFENSHGISSGTV
                     FDSLFAREKLGSTGLGQGIAIPHGRIKGLKEASGAFVRLAQPLPFEAPDGRPVQLIFA
                     LLVPEQATEQHLQILSELAQRFSEREFREKLAAASERAEVRQAFAD"
     gene            6127..7062
                     /gene="hprK"
                     /locus_tag="VIS73_05365"
     CDS             6127..7062
                     /gene="hprK"
                     /locus_tag="VIS73_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEY5762613.1"
                     /translation="MQKISVAQLFEDHRDRLQLTWAAAPGVDRHIELKVNANYGADVV
                     GHLNLIHPERLQVIGEAEIDWATRKNPERLKRLIGDLLTAEPPALIVADGVVPISALV
                     EACEATQTPLLRTPMAASAVIDLLRMYLSRRLADTTSLHGVFMDVLGLGVLITGDSGV
                     GKSELALELISRGHGLVADDVVELARTSPTALEGRCPGLLRDFLEVRGLGLLNIRTIF
                     GETASRRKMRVALIVHLQKPIAGVDAPRLPLDAQTQDILGIAVRKVIIPVAAGRNLAV
                     LLEAAVRNTILQMRGVDSMQEFIARQQRALLEDEL"
     gene            7109..7462
                     /locus_tag="VIS73_05370"
     CDS             7109..7462
                     /locus_tag="VIS73_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HEY5762614.1"
                     /translation="MTRILKTLLAAALGAACISTAAYAADEAKAPTPQQMRMKACNQE
                     AGGKQLKGEPRKEFMRGCLSGKKGEGKGPAMAASDHPECAARANDSKGKPLAGAARAS
                     FMKKCMNEMPMANKP"
     gene            complement(7510..8463)
                     /locus_tag="VIS73_05375"
     CDS             complement(7510..8463)
                     /locus_tag="VIS73_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY5762615.1"
                     /translation="MAAQNTSRVGIVYADISGTTRLFHKLGTEEASYAVDRAIKRMER
                     AIEGFSGRVVKAAAEQWIAEFDAPASAVSAAVEMQTRVNDMLAVSGIRLTVRIGVHWG
                     GTAGTEAGRDAAIEIARRLLTMAGPSQILTCGQTAAALPPELSGMLWSCDDLLLTLPD
                     GAEMQVYRVQWVEADEAAAMQRLETTVVLDPIPGGEASPPAEPGHFCIRAGGRSYLID
                     DRTPQLAIGRDKKHNDIVIRDPKASREHARIERRSDGRCVLIDTSTNGTYVLDGNAET
                     RVRGGELLLGVKGRIAFGHSPRDQGAECLEFERLSKASVAV"
     gene            complement(8510..9403)
                     /gene="ttcA"
                     /locus_tag="VIS73_05380"
     CDS             complement(8510..9403)
                     /gene="ttcA"
                     /locus_tag="VIS73_05380"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEY5762616.1"
                     /translation="MTEPSNTLLRLRRKLERKTGQAIADYRMIEDGDVVLVCLSGGKD
                     SYTLLSLLLGLQARAPVSFRLIVMNLDQKQPGFPAEVLPAYLDSLGVDYRIVEQDTYS
                     IVREKIPEGRTTCSLCSRLRRGIIYRTARELGATKIALGHHRDDLVHTLFLNLFFGGK
                     IKAMPPKLVTDDGAHIVIRPLAYCAEADIARFARGMAFPIIPCNLCGSQDNLQRQKVR
                     EMVDDWERRFPGRIEAVLTALQNVVPSHLADHRLFDFSSLQAGDRARAEGDRVFDDPV
                     DWQNATNLVAEVPAATLLSWS"
     gene            complement(9400..10155)
                     /locus_tag="VIS73_05385"
     CDS             complement(9400..10155)
                     /locus_tag="VIS73_05385"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HEY5762617.1"
                     /translation="MRDSTSPPRRAFLVTGGARRVGAAIVRRLHADGADVIIHYRSSA
                     LAADEVADELNAVRPGSVELLQADLNRLDRLDAFARDAVDCFGRLDGLINNASSFYPT
                     PLGQIGEDDYEDLLGSNFRAPLFLTQYLAGELRRRQGVVVNIIDIHAERPLAGYPLYS
                     AAKGALATLTRALAIELAPAVRVNGVAPGPIDWPEDAQFDEAARAKIVEHTLLKRVGK
                     AEEIAAAVAFLAGEASYVTGQILNVDGGRSVHL"
     gene            complement(10179..10955)
                     /locus_tag="VIS73_05390"
     CDS             complement(10179..10955)
                     /locus_tag="VIS73_05390"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY5762618.1"
                     /translation="MHLPAPAALIFACLLFGGLTACSGNAARPHPAHALAWPLAVLAD
                     AEGERLGIAGLSPEPLWVPLPALRSARRAAERLLAANPGEAPELWVTSNTVPNAMAFR
                     REGVPVVAITAAMADLLGEDEDAWAALFGHELAHLRLAHQQRAGTRAEAVQAGSSVAA
                     ILLAIVGIPFASVVADGAGEVVAKGFSRDDEREADEAGMAYAASAGYSAAGAARLFER
                     LASVDRDRGITLLSTHPGIEERVARARAAGSATAAEAAPR"
     gene            11031..12257
                     /locus_tag="VIS73_05395"
     CDS             11031..12257
                     /locus_tag="VIS73_05395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY5762619.1"
                     /translation="MIGSLAFPTALSMPASHSASPPEPLSSTLMARLRRDADAGDGWL
                     SFARFMEIALYTPMAGYYAGGSRKFGASGDFVTAPEMTPLFAQALAVQVAEIIRRSEA
                     RVIEAGGGSGRLAADLLLALDAAGCAPDEYALLEVSPDLAARQKALLQSTLPAGLAKR
                     CRWLSQLPERFSGVVLGNELLDAMPIHLVRWTASGILERGVAVAGDGLLSWQERPALG
                     RLLAAAEAIADRLAPADGYLSEISLAGPAWVRSLGERIERGALLLLDYGFPEHEYYHP
                     QRDGGTLMCHHRHRAHTDPLIHVGQQDITAHVDFSAIADAAHEAGLAIAGYTAQAPFL
                     MNCGILDALATVDASSLDGIRARSAVQKLLSPAEMGELFKVIALTRGLSDGSTATAGS
                     AESLIGFRSGDRRWRL"
     gene            complement(12275..12532)
                     /locus_tag="VIS73_05400"
     CDS             complement(12275..12532)
                     /locus_tag="VIS73_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762620.1"
                     /translation="MVIIMDMTSGKSEAPRSDEIQSEPAYAEDVLLAGFTEVPAEWRA
                     QDQRRAVAPRLAEVAVEAKASDPDAYMHSVYAAQGLPGRKR"
     gene            complement(12640..13509)
                     /locus_tag="VIS73_05405"
     CDS             complement(12640..13509)
                     /locus_tag="VIS73_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5762621.1"
                     /translation="MKDDVKFQQLPRKSRPQPGKTLARRRFRLGGIEVIAAGPPDGGT
                     PILFVHGAFAGAWMWDEVFLPYFAAAGYEAWGLSLRGHGESEGREIIDHHSIRDYVDD
                     VMTVADAMPAPPVLVGHSMGGFVVQKTLEHRPAPAAVLMASVPPQGLVAASFHLAFSH
                     PDLFAQVNRLLAGADISAEAVADALFAKPLPPELMRRALRQMSPESQRAIWDMSMFNL
                     VSMHAVHRTPLMVLGAEEDRLVPAFVVQSTARAYGVPDHLFTGHGHGFPLEPGGERIA
                     DAVLAWLAKVELA"
     gene            complement(13547..14770)
                     /locus_tag="VIS73_05410"
     CDS             complement(13547..14770)
                     /locus_tag="VIS73_05410"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEY5762622.1"
                     /translation="MQVFAVGGAVRDELLGLPVKDRDYVVVGATPAEMLAQGYQPVGR
                     DFPVFLHPETHEEYALARTERKTAPGYHGFSFHAAPEVTLEEDLARRDLTVNAIARAA
                     DGTLVDPYGGQRDLERRVLRHVGPAFVEDPVRVLRVARFAARFPDFVVADETMALMAD
                     MVRAGEVDALVPERVWQELARGLMAARPSRMFETLRDCGALARLLPEVDSLVGVPQRA
                     EYHPEGDAWTHTMMVVEVAARRDEPLPVRFAALTHDLGKATTHADILPRHHGHEARGA
                     RLIEALAARLRVPADCRDLAVLAAREHGRLHQLDRPGGLNAKTIVDVLEKCDAFRRPD
                     RFAQLLSVAACDHAGRGGVSDEYQAGPRWRRMLVAASAVDAGAVAAECASGSEIGVRV
                     RAARLAAVAAAVERN"
     gene            complement(14770..>15272)
                     /locus_tag="VIS73_05415"
     CDS             complement(14770..>15272)
                     /locus_tag="VIS73_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEY5762623.1"
                     /translation="FRPSVIFGVGDSFLSLFANFVRGLPFFPLGYGQAKFQPVFVGDV
                     AECFLRSIEARESFGKAYDLVGPQVYTLRQLVDYVNTLCGKRCRVIALPEFWALMQAG
                     IMSLLPKPPLTPDNLRSMEVDSVSPDAVMPFGVTPTPLESVAPSYILGRAPRVHYDRM
                     RVRAGR"
BASE COUNT         2746 a         5175 c         4843 g         2508 t
ORIGIN      
        1 ggccgcagcc ccggcttacg cttggcctcg cggtgcgcgg actagcaacg gcagcgatcg
       61 acgtctccga cggactgctc ggcgaccttt cgcacatcct tgagcgctcg gcgctcgccg
      121 ccgaaatcgc catcgacgat cttccgccgc ttcccgccgg cgttgatgca agcctcgcgc
      181 gccgctgcca actggctggc ggcgacgatt acgagctggt gttttcagcg ccgcccaccg
      241 ccggcctcgc gatcgccgcg ctgggccgca gactcgggct accgttgacc cgcttcggcc
      301 gcgttgtcgc cgggtcggcc ggtacaatta cccttcgcga ccccgacggc caggtggtcg
      361 actttgccga gcgcggccat gaccacttcg cctgacatcc gcttcgtttt ttccagccca
      421 tttcacctga tcgcctgcgg cttcggcagc ggcctctcgc cggttgcctc gggcaccgtc
      481 ggcacgctct tcgcgtgggc ctcgtttccg ctgctgcaag gcctctttgg cgatgtcgag
      541 ttgctcgtct ttctggtgct ggccttcctg ttcggcatcc tcgcctgcca ccgcaccggc
      601 cgcgcgctgg gagtcgtcga ccacggggtg atcgtctggg acgagatcgt gccgttctgg
      661 ctggtcctgc tgctgacgcc cgagggctgg ctctggcaaa cagcggcctt cttcctcttc
      721 cgcttcttcg acatcgtcaa gctgcccccg gccggctact tcgaccgcga ggtgaagaac
      781 ggttttggtg tcatggccga cgacctcttc gccgccctgc acacgctgct ggtgatcgcc
      841 gcggcgaagc tctggatgcc gttctggcta ccactcgtgc agccattcct cccgccgttg
      901 tgattttcga ccaggacgca ctcgaaacgc tcgcttcggc ggtcggccag gccctactgg
      961 cgcgcggcga gcgcctcgcg accgccgagt cgtgcaccgg cggctggctt gcccaagcga
     1021 tcaccgcgat ccccggcagc tcagactggt tcgaccgcgg attcgtcacc tacgccaacg
     1081 ccgccaaggt cgaaatgctc ggcgtcgacc cggcaacact ggatcggcac ggcgcggtca
     1141 gcgaggcctg cgcccgcgaa atgctcgccg gcacattgaa gctcagtcag gccgactggg
     1201 ccgtatcgat caccggcatc gccggcccag gcggcggctg tttcgacaag cccgtgggat
     1261 cggtctggtt cgcctttggc gggcccgcgg ccgtcgccga cgcgacgctg cagcgtttcg
     1321 aaggcgaccg acgggcggtt cgtgcgcagg ctgtcgcctt cgctctcgaa accctgcgcg
     1381 gcaagctggc aacgccgatg cgtgcctgag tccatgacgc cgcgatgccc ggcgacttgc
     1441 acagcagcgc ttttactgta taaacttcca gttatcgggg tcggccgctc gtctcgccgg
     1501 cactccaatt cataatgccg cgcccggcac gttttcgagg acaagcaatc atggaagaga
     1561 acaaatccaa agcactggcc gccgccctgg cccagatcga aaagcagttc ggcaagggtt
     1621 cgatcatgaa aatgggcgat ggcgacatcg gcgagccgat cgacgtcgtc tccaccggct
     1681 cgctcgggct cgacatcgca ctgggcgtcg gtggcctgcc gcgcggccgt gtggtcgaga
     1741 tctacggccc ggaatcctcg ggcaagacca cgctgacgct gcaggtcgtc gccgagatgc
     1801 agaagctcgg cggcacggcc gccttcatcg atgccgagca cgcgctcgac ccgggttacg
     1861 cgcagaagct cggggtcaag gtcagcgacc tgctgatctc gcagccggac accggcgaac
     1921 aggcgctgga gatcgctgac atgctggtgc gttccggcag cgtcgacgtt atcgtcatcg
     1981 actcggtggc cgcactggtc ccgcgggccg aaatcgaagg cgaaatgggt gactcgctgc
     2041 ctggcctgca cgcgcgcctg atgagccagg cgctgcgcaa gctcaccgcg aacattaaga
     2101 agacccacac actggtgatc ttcatcaacc agatccgcat gaagatcggc gtcatgttcg
     2161 gcagccccga aacgaccacg ggcggcaatg cactgaagtt ctacgcttct gtacggctcg
     2221 acatccgccg aatcggttcg attaagcggg gtgacgaggt agtcggcaac gaaacgcggg
     2281 tcaaagtggt caagaacaag gtctcgccgc cgttccgcga ggcgctgttc gacattcttt
     2341 atggcgaggg catctcgcgc gaaggcgaaa tcatcgaact cggagtcgca cacaagattc
     2401 tcgacaagtc cggtgcttgg tacgcctata acggcgacaa gattggccag ggcaaggaca
     2461 acgtgcgcga attcctgcgt ggcaatcccg atatggcgcg cgagatcgaa gcgaagatcc
     2521 gcgcggccgt cggcgtgcac accgccgcca agcccgcgga agccgaggcc tgaaaccgat
     2581 ctcgctgcgc aatcaggctt tgcgcctgct ttcgcggcgc gaacattcgc gcgccgagct
     2641 cgcggccaaa ctacaggcaa ccgcgggtga gggggaggat atcgatggcc tgctcgacag
     2701 cctcgaatca agcggcctgc tctccgacgc gcgctacgcc gaaggccggg cacgggttcg
     2761 tggcggtggc ctaggcgacc agcggctggc gcatgagctg cgccagcgcg gggttgccgc
     2821 cgagcttatt gaatcgacac tgtctgcgct gcccgacgaa gccgaccgtg cccgcgcagc
     2881 gtggcagagg cgctttggta ccagaccgct cgatcgagcc gattgggcaa aacaggcgcg
     2941 attcttgcag tcgcgcggct tcgcagtggg tacaattcgt cgggtactcg actcgcccga
     3001 cgaagacgaa ccctagctga tgcccaccga acgcagcacg ctttccgcaa cacaactcaa
     3061 gaaacgctac aagtcgcgca ccgtcgtgca cgacgtttcg ttcgaagtcg cgagcggcga
     3121 ggtcgtcggc ctactgggcc caaacggcgc cggcaagacc acctgcttct atatggtggt
     3181 cggcctgatc gccgcagacg gcggcgaagt ccgcctcgac gagcactcgc tcacccatct
     3241 gccgatccat cgtcgcgccc gcctcggcct ctcgtatctg ccgcaagaag cctcggtgtt
     3301 ccgcaagcta acggtgaccg aaaacatcct ggctgtgctc gagctgcagg gcctgggacg
     3361 cgaagcgacc gaggcccgcc tcgaaagcct gctcgaggaa ctgcacatcg ctcacatccg
     3421 caacaccaac gcggcggcgc tgtccggcgg cgagcgccgc cgggtcgaga tcgcccgcgc
     3481 gctggcgacc gaaccgcgct tcatcctact cgacgagccc tttgccggcg tcgatccgat
     3541 cgccgtcctc gatatccaga agatcatccg ctttctcaag ggccgcggca tcggcgtgct
     3601 gatcaccgac cacaatgtcc gcgagacgct gggcatctgt gaccgcgcct gcatcatcaa
     3661 cgccggcgaa gtattggcag cgggccggcc cgacgagatc atttatgatg aggatgtccg
     3721 gaaagtctat ctaggcgaga acttccgcct ctagggcctt accgtcgcca actaacgaaa
     3781 gtcatagatt cgcgcgatga aaccggcact gcagctaaaa ctctcccagc atctggcgct
     3841 gacgccccag ctgcagcagt cgattaagct gcttcagctg tcgacgctcg agctcaatca
     3901 ggaactcgag cgctacctgc tcgagaaccc gttgctcgag cgcgacgaag aagagaccgc
     3961 cgccgaggcg ccaagggacg cggaccggag caatggcgag gctgcttcgt ctgcgaccag
     4021 cgacgaaagc gccggcgagc gcggcagcga aggcggcgac agcggcgagg ccgcacccgc
     4081 tgccgatgac acgccgaccg acttcggcga cgagtggctc gacgaaagcg ggtcctacgg
     4141 ctccaagagc ggacggcagg acgacgacga ggatttcgac ccgcaggacg cccagtccag
     4201 ctccacctcg ctgcgcgagc acctcgactg gcaggtggca ctgacgccgc tttccgaccg
     4261 cgaccggacg ctggtccgcg tactcatcga ggcgctcgac gacgacggct acctgaccca
     4321 ggcacttgaa gagctggctg aatccctgcc cgaggaggcc gaagtcgggg tcgatgagct
     4381 gttggtcgcg ctgcgcatcc ttcagtccct cgacccgacc ggtgtcggcg cccgcaatgc
     4441 ccgcgagtgc ctgtcgcttc agctcaaggc acgcgaaccg ggaacgattc gcgacctggc
     4501 cctgaacctc gtcgccaatc atctcgacca gatggccacc agggactttg cgaagatcaa
     4561 aaagggactg ggctgcgacg acgaccagct gcgctcgacc atggccatga ttcgcggact
     4621 cgatccacgc cccggcgcca aatacgcgcc gatggatacc cgctacatca cccccgatgt
     4681 gatcgtccgc aaggtccgcg gaaaatgggt tgccagcgtc aacggcgacg ccttcccgcg
     4741 gcttcgcgtc aatcgcctct acgcagagat tctttcgcgc cagcgcgggt cgggtctggc
     4801 cggtcagctg caggaggcgc gctggctgat caagaacgtc cagcagcgct tcgataccat
     4861 ccagcgagta acccaggcca tcgtcgaccg gcagcggcag ttcttcgagc acggcgaagt
     4921 cgcgatgcgg ccactggtgc tgcgcgagat cgccgatatc cttggcctgc acgagtcgac
     4981 catttcgcga gtcacctcac agaagtacat ggcaacaccg cgcggaatct ttgaattgaa
     5041 atactttttc ggcagccacg tcgcgaccga agcgggcggc gcttgctcgg caacggcaat
     5101 tcgggcgctg atcaagcaga tggttgcagc agaaaacccc cagaaaccgt tgtctgacag
     5161 ccaaatttca gacattctcg ggcaacaggg gattgtcgtg gcgcgacgga cgatagcaaa
     5221 ataccgtgaa gcgctgagta ttcctcccac caacctacgc aagtcgctat aagtgacaag
     5281 gagcgcaaca tgaaccttca catcaccggc caccacctcg aagtgacgcc agcattacgc
     5341 gactatgtga ccaccaagct cgaaaaggtc acccggcatt tcgaccacgt catcgacgtc
     5401 tcggtaacgc tatcggtgga gaaactgaag cagaaggccg aagtcacgct gcacgttcga
     5461 ggcaaggaca ttttcgtcga gtcggacgac agcgaccttt acgctgcgat cgacgcgctg
     5521 gtcgacaagc tcgaccgtca ggttcttaag tacaaacaga agcttcagga ccatcaccgc
     5581 ggcgatcgca tcacgcacca catcgctgaa tgaaccgttc cacccgcccc gggattgcgc
     5641 ctagcacgca accccgggga atctagcccg gtccccaatg tcactactcg ccagcctgct
     5701 ccccgaaaac cacgtccttc ttgacgtcga gatcaccagc aagaagcgct tgttcgagta
     5761 catcggcctg ctcttcgaga actcccacgg gatttccagc ggaacagtct tcgacagcct
     5821 gtttgcccgc gagaaactcg gctcgaccgg cctggggcag ggaatcgcca ttccccacgg
     5881 acggatcaag ggcctcaagg aggccagcgg cgccttcgtc cgcctggcac aaccgctgcc
     5941 tttcgaggca cccgacggac gaccggtgca gctgatcttt gcgctgctgg ttcccgaaca
     6001 ggcgaccgag caacacctgc agatcctctc cgaactggca caacgttttt ccgagcgcga
     6061 attccgcgag aagctggccg ccgccagcga acgcgccgaa gtccggcaag ccttcgccga
     6121 ctgactgtgc agaaaataag cgtcgcgcag ctcttcgaag accaccgcga tcgtctgcaa
     6181 ctgacctggg ccgccgcgcc cggcgtcgac cgccacatcg aactcaaggt caacgccaac
     6241 tacggggcgg acgtcgtcgg tcacctcaac ctaatccatc ccgagcgtct gcaggttatc
     6301 ggcgaagccg agatcgactg ggccacgcgc aagaatccgg agcgcctgaa gcgcttgatc
     6361 ggcgacctgc tgacggccga accgccggcg ctgatcgtcg ccgacggcgt cgtgccgatc
     6421 agcgcgctgg ttgaagcctg cgaagcgacg caaacaccgc ttctgcgcac accgatggcg
     6481 gcatcggcgg tcatcgacct gttgcgcatg tacctgtcac gacgcctggc cgacacgacc
     6541 tcgctgcacg gcgtgttcat ggatgtcctc gggcttggcg tgctgatcac cggcgactcg
     6601 ggtgtcggca agagcgaatt ggcgctggag ttgatctcgc gcggccacgg gctcgttgcc
     6661 gacgacgtcg tcgagctggc acgaacgagc ccgacggcgt tggagggccg ctgccccggc
     6721 ctgttgcgtg acttcctcga ggtccgcggc ctgggtctgc tgaacatccg gacgatcttc
     6781 ggcgaaaccg cctcccgccg gaaaatgcgc gtggcgctga tcgtgcacct gcaaaagccg
     6841 atcgccggcg tcgacgcccc gcggctaccg ctcgacgcac aaacgcagga catccttggg
     6901 atagccgtgc gcaaggtcat cattcccgtc gccgccggcc gcaacctggc cgtcctgctc
     6961 gaagccgccg tccgcaacac gatcctgcag atgcgcgggg tggacagcat gcaggagttc
     7021 atcgcacgcc agcagcgtgc cctgctcgaa gacgagttat gattctcgca ttgggcggca
     7081 gtcgtcgcct tctttcgggg aaaagaccat gactcgcatt ttgaagacgc tccttgcggc
     7141 cgcactcggg gccgcctgca tcagcaccgc tgcctacgct gccgacgagg ccaaggcgcc
     7201 aacgccacag caaatgcgca tgaaggcctg caaccaggaa gccggcggaa aacagctcaa
     7261 gggcgagcca cgcaaggaat tcatgcgcgg ctgcctgtcc ggcaagaaag gggagggcaa
     7321 gggaccggcg atggccgcca gcgatcaccc ggaatgcgcc gcccgcgcca atgactccaa
     7381 ggggaagccg ctggccggcg ctgcccgcgc ctcgttcatg aagaaatgca tgaacgaaat
     7441 gccgatggcc aacaaaccct agctgtagac cgcccagcct gcggcaccgg actcacttcg
     7501 aaccggcctt taaacggcca ccgacgcctt gctgagacgt tcgaactcca gacactcggc
     7561 accttgatca cgcggtgagt ggccgaaggc gatccgccct ttgaccccga gcagcagttc
     7621 acccccgcgc acgcgggttt ccgcgttgcc gtcaagaacg taagtaccgt tggtgctggt
     7681 atcgatcagc acgcagcgtc cgtcgctgcg ccgctcgatc cgcgcgtgct cacggctagc
     7741 cttcgggtcg cggatgacga tatcgttgtg cttcttgtcg cggccgatcg ccagttgcgg
     7801 ggttctatcg tcgatcaggt aggagcggcc gccggcacga atgcagaaat gccccggctc
     7861 cgccggtggc gacgcctcgc cacccggaat cgggtcgaga acgacggtgg tctcaagccg
     7921 ctgcatcgcg gcggcctcgt cggcttccac ccattgcacg cgatagacct gcatctccgc
     7981 gccatcgggc agcgtcagca gcaggtcgtc gcaggaccag agcatgccgc tcagctccgg
     8041 cggcaacgcc gcggcggttt gcccgcaggt cagaatctgc gaaggtccgg ccatcgtcag
     8101 caggcgccgc gcgatctcga tcgccgcatc gcgcccggct tcggttccag ccgtgccgcc
     8161 ccaatggacg ccgatccgga cggtgagcct gatgcccgag acggcgagca tgtcgttgac
     8221 gcgagtctgc atctccacgg cggccgagac cgctgacgcc ggcgcatcga actcggcgat
     8281 ccactgttct gcggcagcct ttaccactcg gcccgagaaa ccctcgatcg cgcgctccat
     8341 gcgcttgatg gcgcgatcaa ctgcgtagct ggcctcttcg gtgccaagct tgtgaaacag
     8401 cctcgtcgtg cccgaaatgt cagcgtaaac gatgccgact ctcgacgtat tctgtgctgc
     8461 cattattgtt cccctgcgcg cctgtgccgc aacctgctca cggccggcac tatgaccaag
     8521 ataatagcgt agcggccggc acttccgcga ccaaattggt cgcgttctgc cagtcaaccg
     8581 gatcgtcaaa gacccgatca ccctcggccc tggcgcgatc accagcctgc aggctggaga
     8641 agtcgaacag gcgatggtcg gccaggtgcg agggcacgac attttgcaag gcggtcagta
     8701 cggcctcgat ccgcccaggg aagcgacgct cccagtcgtc gaccatctca cgcaccttct
     8761 gtcgctgcag gttgtcctga gaaccgcaga gattgcaggg gatgattgga aacgccatgc
     8821 ctctggcgaa gcgcgcgatg tcggcctcgg cgcaataggc cagcgggcgg atgacgatgt
     8881 gcgcaccgtc gtcggtgacc agtttcggcg gcatcgcctt gatcttgccg ccgaagaaca
     8941 ggttgaggaa cagcgtgtgc acgagatcgt cgcgatgatg gcccaacgcg atcttggtcg
     9001 ccccgagttc gcgcgcggtt cggtagatga ttccgcggcg cagccgcgag cagagcgagc
     9061 acgtcgtccg cccctcggga atcttttcgc gaacgattga ataggtgtcc tgctcgacga
     9121 tccggtaatc aacgccaagc gaatcaagat aggccggtag cacctcggcg ggaaagcccg
     9181 gctgcttctg gtcgaggttc atcacgatca gccgaaacga cacgggcgcg cgcgcctgca
     9241 gacccagcag cagcgacagc agcgtgtagg agtccttgcc gcccgagagg cagacgagga
     9301 cgacatcgcc atcctcgatc atccggtagt cggcaatcgc ctggccggtc ttgcgttcga
     9361 gcttgcgccg caggcgcagc aacgtgttcg agggttcagt cacagatgga cgctcctgcc
     9421 gccgtctacg ttgagaatct ggccggtgac atagcttgcc tcgccagcca ggaaggcgac
     9481 cgccgcggcg atctcttccg ccttgccgac ccgtttcagc agcgtgtgtt cgacgatctt
     9541 ggcgcgggct gcctcgtcga actgcgcgtc ctcgggccag tcgatcggac ccggcgccac
     9601 gccattgacg cgcaccgctg gcgccagttc gattgccagc gcccgcgtca gcgtcgccag
     9661 ggcgcccttc gccgccgagt aaaggggata gcccgctaag ggccgctccg cgtgaatgtc
     9721 gatgatgttg acgacgacgc cctgcctccg gcgtagttca ccggccagat actgcgtgag
     9781 gaaaagcggc gcgcggaagt tgctgcctaa gaggtcttcg tagtcgtcct cgccgatctg
     9841 cccaagcggc gtcgggtaga aactcgacgc gttgttgatc agcccgtcga gtcgcccgaa
     9901 acagtcgacg gcgtcgcggg caaaggcgtc gagtcggtcc aggcgattga ggtcggcctg
     9961 cagcagttcg acggaaccgg ggcgcacggc attaagctca tcggcgacct cgtcggcggc
    10021 cagtgccgag ctgcgatagt gaatgatgac atcggcaccg tcggcgtgca gtcggcgcac
    10081 gatcgccgcc ccgacgcgac gagccccgcc cgtcaccaga aatgcccgcc gcggcgggct
    10141 cgttgaatca cgcattggct tccttcccgg cagtgattct atcgtggcgc cgcctctgcc
    10201 gccgtagcag accctgcggc gcgggcgcgc gcgacccgct cctcgatgcc cgggtgagtc
    10261 gacagcagcg tgattccccg gtcgcgatcg acgctcgcca gccgttcgaa caggcgcgcc
    10321 gcgcctgccg ccgaataacc ggcgctcgcc gcataagcca ttccggcctc gtcggcttcg
    10381 cgctcatcgt cgcgcgaaaa gcccttggcg accacctcgc ccgccccatc ggcgaccacc
    10441 gaggcgaagg gaatgccgac gatcgccagc aggattgccg caacgctcga tccggcctgc
    10501 acggcttcgg cgcgggtgcc ggcacgctgc tggtgtgcaa ggcgcaggtg cgctagctcg
    10561 tgcccgaaaa gcgccgccca ggcatcttca tcctcaccca gcaggtcggc catcgcagct
    10621 gtgatcgcaa ccaccggcac cccctcccgg cggaaggcca tcgcgtttgg gaccgtgttg
    10681 ctggtcaccc acagctcggg tgcctcgccg ggattggcgg cgagcagtcg ctcggctgca
    10741 cggcgtgcgc tgcgtagcgc cggcagcggg acccacagcg gctccgggga caatccggca
    10801 atgccgagcc gctcgccctc ggcgtcggca agcacggcga gcggccacgc cagtgcgtgc
    10861 gccggatgcg ggcgcgccgc atttcccgag caggccgtca ggccgccgaa aagcaggcag
    10921 gcaaagatca gggcagcggg cgcaggcagg tgcatcgggt ctcgcgtatg ggtcgcacag
    10981 gcgaatactc cggcctgact ggcggcttgc caaggcccgc cgggtcaacg atgatcggca
    11041 gcctcgcctt tccaaccgcc ttgtcgatgc ctgccagcca ctccgccagc cctcccgagc
    11101 cgctctcgtc gacgctgatg gcgcgcttgc gccgcgacgc cgacgccggc gacggctggc
    11161 tgtccttcgc gcgcttcatg gagatcgccc tctatacgcc gatggccggc tactacgccg
    11221 gcggcagccg caagttcggc gccagtggcg acttcgtcac cgcaccagag atgaccccgc
    11281 tgtttgccca ggcgctcgcc gtacaggtcg cggagatcat caggcgcagc gaggcgcgcg
    11341 tgatcgaggc tggcggcgga tcgggccgac tggccgccga cctgctgctg gcgctcgacg
    11401 ccgccggttg tgcacccgac gagtacgcgc tgctcgaagt gtcgcccgat ctcgcggcga
    11461 ggcaaaaagc ccttctgcaa tcaacgcttc cggccggcct ggcgaagcgc tgccgatggc
    11521 tctcgcagtt gcccgaacgc ttctcgggcg tcgtgcttgg caatgaactg ctcgatgcga
    11581 tgcccattca tctcgttcgc tggacggcgt cggggattct cgagcgcggt gttgccgttg
    11641 ccggcgacgg attgctttcc tggcaggagc gaccggcgct gggccgcctg ctggcggcgg
    11701 ccgaagccat tgccgaccgc ttggcaccag ccgacggtta tctctccgaa atttcgctcg
    11761 ccggcccggc ctgggttcgc agcctcggcg aacggattga gcgtggcgcc ctgctgctgc
    11821 tcgactacgg ttttcccgag catgagtact accacccgca acgcgatggc ggcacgctga
    11881 tgtgccacca ccggcatcgc gcccacaccg acccgctgat ccacgttgga cagcaagaca
    11941 tcaccgcgca cgtggatttc tcggcgatcg ccgacgcggc gcacgaggct ggtctcgcca
    12001 tcgccggcta cacggcgcag gcgcccttcc tgatgaattg cggaattctc gacgcactgg
    12061 cgacggtgga cgcgtcgagc ctggacggca ttcgcgcacg cagcgccgtt cagaagctgc
    12121 tgtcgccagc ggagatgggg gagttgttca aggtaattgc cctgaccagg ggcctgtcag
    12181 acgggtcgac ggcgacagcc ggcagtgcgg aatcactgat cggattccgc agcggcgatc
    12241 gccgctggcg gctctaggcg ccgccagcgc gacttcagcg cttgcggccg gggagccctt
    12301 gcgcggcgta gacgctgtgc atataggcgt cggggtcact ggccttggct tcgactgcaa
    12361 cctcggccag ccgcggcgct actgcccgcc gctgatcctg cgcccgccat tccgccggca
    12421 cttcggtgaa gcccgccagt agaacgtcct cggcgtaagc cggttcgctc tgaatttcgt
    12481 cactgcgcgg cgcttcgctc tttccgctcg tcatgtccat gatgatcacc attcaatgca
    12541 cctcccgatt ggtcggtacg acactcaacg acagtgtcgc gcaaaagttg aagcaaagtc
    12601 caacgacatt gtccagttcg cggaacaatt ccgcatcgct caggcgagtt ccaccttcgc
    12661 cagccaggcc agcaccgcat cggcgatcct ctccccgccc ggttcgagcg gaaagccatg
    12721 accgtgcccg gtgaacaaat ggtcggggac accgtaggcg cgcgccgtcg actggacgac
    12781 aaaggccggc accaaccggt cttcctcggc gccgagcacc atcagcggcg tgcggtgcac
    12841 ggcgtgcatc gacaccaggt tgaacatgct catgtcccag attgcccgct gcgactcagg
    12901 gctcatctgc cgtagtgccc gacgcatcag ttcgggaggg agcggcttgg cgaacagcgc
    12961 atcggcaacc gcctcggcag agatgtcggc accagcaagc aggcggttga cctgggcaaa
    13021 cagatcggga tggctgaagg cgaggtggaa ggaagcggcg accaggccct gcggcggcac
    13081 agaggccatg agcacggctg ccggcgccgg gcgatgctca agcgtcttct gcaccacgaa
    13141 gccgcccatc gaatggccga cgagtaccgg cggcgccggc atcgcatcgg cgactgtcat
    13201 cacgtcatcg acgtaatcgc ggatgctgtg gtggtcgatg atttcgcgac cctcgctctc
    13261 gccgtggccg cgcagcgaca gcccccaggc ttcgtagccg gcggcggcga agtagggcag
    13321 gaagacctcg tcccacatcc aggcgccggc aaaggcgcca tgcacgaaaa ggatgggcgt
    13381 gccgccatcg ggcgggccgg cggcgatgac ctcgattcca ccgagccgga agcgacgacg
    13441 cgccagagtc ttgcccggtt gtggtcgcga cttgcgcggc agctgctgga atttcacatc
    13501 gtctttcatg gtggacgagt gacagacgcc taatccaccc ggacgttcag tttctttcga
    13561 ccgcggcggc aaccgcggca agccgcgcgg cgcgcacgcg aactccgatc tccgatccgg
    13621 acgcgcactc cgcggcgacg gccccagcgt cgacagcgct tgctgccacg agcatccgcc
    13681 gccagcgggg tccggcctga tattcgtcgc tcacgccgcc gcggccggcg tgatcgcagg
    13741 ccgctacaga cagcagttgg gcgaaacggt ccggtcggcg aaacgcgtcg cacttttcca
    13801 ggacatcgac gatggtcttc gcgttcagcc cgccgggacg gtcgagttga tgcagacgtc
    13861 cgtgctcgcg cgccgcgagc acagccaggt cgcggcagtc ggcggggacc cgcaggcggg
    13921 cggcaagcgc ttcgatcagc cgcgcgccgc gcgcttcatg cccgtgatgg cgcggcagga
    13981 tatccgcgtg tgtcgtcgcc ttgccgaggt cgtgcgtcag cgccgcgaag cggaccggca
    14041 gtggttcgtc acgccgtgcg gcgacctcga cgaccatcat cgtgtgcgtc catgcgtcgc
    14101 cttccgggtg gtactcggca cgttgcggaa cgccgaccag cgaatcgact tcgggcagca
    14161 gacgggcgag cgcaccgcag tcccgcaggg tctcgaacat gcgagacgga cgcgctgcca
    14221 tgaggccgcg cgcgagctcc tgccagacgc gctcgggtac cagcgcatcg acttcgccgg
    14281 ctcggaccat gtcggccatc agcgccatcg tttcgtcggc aaccacaaaa tcgggaaacc
    14341 gcgccgcgaa gcgcgcgacg cgcagaacgc gcaccggatc ctcgacgaaa gccgggccga
    14401 catgacggag cacgcgccgt tcgaggtcgc gctggccacc gtaggggtct accagcgtgc
    14461 cgtcggcagc gcgggcaatg gcgttgaccg tcaggtcacg acgcgcgagg tcttcctcaa
    14521 gcgtgacttc gggtgccgca tggaagctga agccgtgata gcccggcgcg gtcttgcgct
    14581 cggtacgcgc cagcgcgtac tcctcgtgcg tctcgggatg caggaatacc gggaagtcac
    14641 ggccgaccgg ctggtagccc tgcgccagca tctcggcagg tgtggcgccg acgacaacgt
    14701 aatcgcgatc cttgaccggc agtccgagca gctcgtcgcg gaccgcgccg ccgacggcaa
    14761 aaacctgcat caacggccgg cgcgcacccg catccggtcg tagtgcaccc gcggcgcgcg
    14821 accgaggatg tagctcggtg ccaccgactc gagcggcgtc ggcgtcacgc cgaagggcat
    14881 gacggcgtcg gggctgacgc tgtcgacttc catcgagcgc aggttgtcgg gggtcagcgg
    14941 cggcttaggc agcagcgaca tgatgccggc ctgcatcaac gcccagaact cgggcagggc
    15001 gatgacgcgg cagcgcttgc cgcacagcgt attcacgtag tcgaccagct ggcgcagcgt
    15061 atagacctgc gggccgacga ggtcgtaggc cttgccgaag gactcgcgtg cctcgatgct
    15121 gcgcaggaag cattcggcga catcgcccac gaaaaccggc tggaactttg cctgcccgta
    15181 accgagcggg aagaagggaa ggccgcgaac aaagttggcg aacagcgaga ggaagctgtc
    15241 gccgacaccg aaaatgaccg acgggcgaaa ga
//
LOCUS       DATDAA010000164         5712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_431653,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000164 DATDAA010000000
VERSION     DATDAA010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431653"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VIS73_05420"
     CDS             complement(<1..1066)
                     /locus_tag="VIS73_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5762624.1"
                     /translation="MISSLLKKVFGSRNDRLLRQFRQGVTRINALEAEIAALDDPTLQ
                     GKTAEFRGRLEKGETLDSLLPEAFAVVREAAKRTLGMRHFDVQLIGGMVLHQGKIAEM
                     RTGEGKTLMATLPAYLNALAGKGVHVVTVNDYLAKRDAEWMGRVYGFLGLTVGNNLSQ
                     MPHDEKQAAYTSDITYGTNNEFGFDYLRDNMVYRAGDRVQRGLAFAIVDEVDSILIDE
                     ARTPLIISGQAEDHTELYIRLNKVAPLLKRCEEENGPGDYWVDEKAHQVLLTEEGHEN
                     AENILAQQGLLAEGRSLYEPGNILLMHHLYAALRAHTLYNRDQQYVVQNNEIIIVDEF
                     TGRLMPGRRWSEGLHQAVEAK"
     gene            complement(1239..2186)
                     /locus_tag="VIS73_05425"
     CDS             complement(1239..2186)
                     /locus_tag="VIS73_05425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY5762625.1"
                     /translation="MHIIVVSDRLSSARTLTLTGGHMLAGGIALLLLVVTMAAGFSYL
                     ALRHAAEVKMPFLDSMMSSLHEREMKRTQEFVRDNIAAIAGKLGQVQAQLVHLDSIGE
                     RLASLTGIKPGDVPKAEAPSPTGQGGPLIELSQPIGTGDLDEQIEKLILAVEHRTDFL
                     AAVESSLIEKRSTASRMPTALPVRANYNSSAFGWRIDPFTGLRAMHEGVDFSARIGTP
                     IAAAAGGVVVSAMVHPQYGNLVEIDHGNGYSTRYAHCNEILVVQGQVVKRGQQVATVG
                     DTGRSTGPHLHFEVRYRGAAVNPTQFLKRSGQSDALARR"
     gene            2210..2659
                     /locus_tag="VIS73_05430"
     CDS             2210..2659
                     /locus_tag="VIS73_05430"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEY5762626.1"
                     /translation="MPELRPLDACIQGAPTLSQVLAHAKLLARAQQILASVAPAALVA
                     ASRVANIRRGELIIHADNGAVAVKIRQIGARLCRELSFQGIDCTAVNVVVQDGRRPVD
                     PPAVAVRALSEGGRASLSALAEGLPDGDPLRQRLSELLARSARPEGE"
     gene            complement(2640..2834)
                     /locus_tag="VIS73_05435"
     CDS             complement(2640..2834)
                     /locus_tag="VIS73_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762627.1"
                     /translation="MLILRLLAVVTILLVAGGVVGYFMTGNQRYLRFSWRVFITAAAI
                     ALAAFALLAIERMAVIPLPV"
     gene            complement(2857..3783)
                     /gene="lpxC"
                     /locus_tag="VIS73_05440"
     CDS             complement(2857..3783)
                     /gene="lpxC"
                     /locus_tag="VIS73_05440"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY5762628.1"
                     /translation="MLRQRSLKSAIRAAGVGLHSGVKVNMTLRPAAPDTGIVFRRVDL
                     AQPVDIPANATAVGDTRMCTVLECNGAKVGTIEHVMSALAGLGIDNAYIDLDAAEVPI
                     LDGSAAPFVFLIQSAGIAEQAAPKRFIRVRKPVEVRETDASGDKWARLEPYDGFRLSF
                     SIDFRHPAIDGTAQTVRVDFAEQSYLREVARARTFGFMHDVEFLRDHGLALGGGLENA
                     VVLDEYRVLNPDGLRYADEFVKHKVLDAIGDLYLLGHPLLADFTAHKSGHALNNRLAR
                     ALINDPEAWELVSFDDSATAPAGVTRWLAMHA"
     gene            complement(3875..5035)
                     /gene="ftsZ"
                     /locus_tag="VIS73_05445"
     CDS             complement(3875..5035)
                     /gene="ftsZ"
                     /locus_tag="VIS73_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY5762629.1"
                     /translation="MFEIIEKDSGSTIIKVIGVGGAGGNAVDHMAREGVQGVEFLAAN
                     TDAQALGRSLAHVKLQLGKSGLGAGAKPEAGRVSAIEQREQIAAALTGANMVFITAGM
                     GGGTGTGAAPIVAEVAREMGILTVAVVTKPFSFEGKRIKIAEAGIAELTQHVDSLIVI
                     LNDKLMEVLGEDAAVDECFRAADDVLRNAVGGIAEIITYPGLVNVDFEDVRTVMGEMG
                     MAMMGSAAASGMDRARIAAEKAVASPLLEGVNLTGARGVLVNITSSRNLKMKEVNEVM
                     STVRAFAADDAHIIFGAVYEEGMEDELRVTVVATGLGRAEGARKLTVVDSDSVSNART
                     GTYDAVASGNYDELAATPAVFRNARAVAKVDAMASGGLDHYEIPAFLRKQAD"
     gene            complement(5068..>5712)
                     /gene="ftsA"
                     /locus_tag="VIS73_05450"
     CDS             complement(5068..>5712)
                     /gene="ftsA"
                     /locus_tag="VIS73_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY5762630.1"
                     /translation="LIDDEKDLGVCLVDIGGGTTDLAIFTQGAIRHTAVIPIAGDQIT
                     NDIAMALRTPTQEAEEIKRRHGVALADLADAEENIEVAGVGDRPPRRLSKRALADVIQ
                     PRVEELFELIQAEIRRSGFEELLSSGIVLTGGAAVMPGMVDLGEEIFHMPVRIGAPRY
                     EGGLADVVRTPRLATAYGLLLEAQSQRRRGLKVREARNLRQVLQRMRAWFEKNF"
BASE COUNT         1010 a         1862 c         1774 g         1066 t
ORIGIN      
        1 ccttcgcctc gacagcctga tgcaggccct ccgaccagcg gcggccgggc atcaggcgcc
       61 cggtgaactc gtcgacgatg atgatctcgt tgttctgcac cacgtactgc tggtcgcggt
      121 tgtacagcgt gtgggcgcgc agcgccgcat acaggtggtg catgagcagg atgttgccgg
      181 gctcgtagag gctgcggccc tcggccagca atccctgctg ggcaagaatg ttctcggcgt
      241 tctcgtgtcc ttcttcggtc agcaggacct gatgcgcctt ctcatcgacc cagtaatcgc
      301 caggaccgtt ctcttcctcg cagcgcttga gcagcggcgc gaccttgttc aggcggatat
      361 agagctcggt gtgatcctcg gcctgtccgg aaatgatcag cggcgtacgc gcctcgtcga
      421 ttaggatcga atcgacttcg tcgacgatgg cgaaagccag gccacgctga actcggtcac
      481 cggctcgata gaccatgttg tcgcgcaggt agtcaaagcc gaattcattg ttggtgccgt
      541 aggtgatgtc agacgtgtag gcggcctgct tttcgtcgtg gggcatctgc gaaaggttgt
      601 tgccgaccgt aaggccgaga aacccgtaga cgcgccccat ccattcggca tcgcgctttg
      661 ccaggtaatc gttgacggta acaacgtgca cacccttgcc ggccagggca ttcagatagg
      721 ccggcagcgt cgccatcagc gtcttgccct cgcccgtccg catttcggcg atcttgccct
      781 gatgcaggac catgccgccg atcagctgaa catcgaagtg gcgcatgccc aacgttcgct
      841 tggccgcctc gcggaccacg gcaaaggcct ctggcaggag cgagtcgagt gtctcgccct
      901 tctccaggcg gccgcggaat tcggcggtct tgccttgcag ggtcggatcg tcgagtgcgg
      961 cgatctcggc ttcgagcgca ttgatgcgcg tgacgccctg acggaattga cgcagcaacc
     1021 ggtcgttgcg gctgccgaag acttttttca ggagactgga gatcattgat tggccgggaa
     1081 aagctgcgcg cgaaaggcgc ggattctagc atggcggccg actcagccgg gtctgcggcg
     1141 cagcggtcgc ctatgccttg cggatgacga gcatcggcac aaggcgaaaa agcaaaacgc
     1201 cgctcaaggc ggcgtcaagg gaagcggggg tgaacagctc agcgccgtgc caacgcgtct
     1261 gattggcccg accgcttgag gaactgggtg gggttcaccg cggcgccacg gtagcggact
     1321 tcgaaatgca ggtgcggccc cgtcgaccgc ccggtatcac cgaccgtcgc aacctgctgc
     1381 ccacgcttga ccacctgccc ctgcaccacg aggatctcgt tgcagtgggc gtagcgcgtc
     1441 gagtagccat tgccgtgatc gatctcaacc agattcccgt actgcgggtg gaccatcgcc
     1501 gagaccacta cgccaccggc cgctgccgca atcggcgtgc cgatccgcgc cgagaagtcg
     1561 acgccttcat gcatcgcccg caaaccggta aacgggtcga ttcgccagcc gaatgccgag
     1621 gaattgtagt tggcgcgcac cggcaacgcg gtcggcatcc ggctcgccgt gctgcgcttt
     1681 tcgatcagcg atgattcgac cgccgcgagg aaatctgtgc ggtgttcgac ggcaagaatc
     1741 agcttttcga tctgctcatc gaggtcgccg gtgccgatcg gctgcgaaag ttcgatcagc
     1801 gggccaccct gccccgttgg cgacggcgcc tccgcctttg gcacgtcgcc gggcttgatg
     1861 ccggtcagcg aggccagccg ctcgccaatc gagtcgaggt ggacgagttg tgcctgaacc
     1921 tgaccaagtt tcccggctat ggccgcgatg ttgtcgcgga cgaactcctg ggtccgtttc
     1981 atctcgcgct catggagtga gctcatcatg ctatcgagaa acggcatctt cacttcggcg
     2041 gcatggcgca aggcgagata ggagaaacct gccgccatcg tgacaacgag cagcagcagt
     2101 gcaatgccgc cagcgagcat gtgcccgccg gtgagtgtca gcgtccgggc cgacgacaag
     2161 cggtccgata cgacgataat atgcacttca acctccttcg cccgacgtga tgccggaact
     2221 acgtcctctc gacgcctgta ttcagggcgc cccaactctg tcgcaggtgc tcgcccacgc
     2281 caagctgctc gcgcgagccc agcagatcct tgcgagcgtc gctcccgcgg cgctggtcgc
     2341 ggcaagccgc gtggccaaca tccgtagggg cgaactgatt atccacgccg acaatggcgc
     2401 ggtcgcggtg aagatacgcc agatcggcgc tcgtctctgc cgcgaattat cgttccaagg
     2461 tattgactgt acagcggtta atgttgtcgt gcaagatggc aggcggcccg tcgacccgcc
     2521 tgcggtggcg gttcgggcgc tttccgaagg tggtcgggcg agcctctcgg cgctcgccga
     2581 aggactccct gacggagacc cgttgcggca acgcctgtcg gaactgcttg cgcgttccgc
     2641 tagaccggaa ggggaatgac cgccatccgt tcaatcgcca gcagagcgaa agccgccagc
     2701 gcgatggcag cggcggtgat gaacacccgc caggaaaacc gaaggtagcg ttggttgccg
     2761 gtcatgaagt agccgaccac cccgccggca accagcagaa tcgtcaccac cgcgagcagg
     2821 cggagaatca gcaccactca gtccgtccag ccttgatcag gcatgcatgg cgagccagcg
     2881 agtcacgccc gccggagctg tcgccgaatc gtcgaagctc accagttccc aggcttccgg
     2941 gtcgttgatc agcgcgcgcg ccaagcggtt gttcagcgcg tgcccggact tgtgcgcggt
     3001 gaaatcggca agcaaagggt ggccgagcag gtagaggtcg ccaatggcgt ccagaacctt
     3061 gtgcttgacg aactcgtcag cgtagcggag tccgtcgggg ttaagcacgc ggtactcatc
     3121 tagaaccacg gcgttctcga ggccaccgcc gagcgccagc ccatgatcgc gcagaaactc
     3181 tacgtcgtgc atgaaaccaa aggttcgcgc gcgggccact tcgcgcagat acgactgttc
     3241 cgcaaagtcc acgcggaccg tctgcgcggt gccgtcgatc gccggatggc gaaagtcgat
     3301 actgaaactg aggcgaaaac cgtcgtatgg ctcgagacgc gcccacttgt caccggacgc
     3361 atcggtctcg cgcacctcca ccggtttcct gaccctgatg aatcgcttcg gcgctgcctg
     3421 ctccgctatc ccggcagact ggatcaggaa gacgaagggg gctgccgaac catcgagaat
     3481 cggcacttcg gccgcgtcca ggtcgatgta ggcgttgtcg attcccagtc cggccagcgc
     3541 cgacatcacg tgttcgatgg tgccaacctt cgcaccattg cactcgagca ccgtgcacat
     3601 gcgcgtgtca ccgaccgccg tggcgttggc gggtatgtcg acgggttgcg cgagatcgac
     3661 ccggcggaaa acgattcccg tatcgggtgc agccggccgc agcgtcatgt tgaccttgac
     3721 gccactgtgc aggccgacac cggcggcgcg gatggcgctc ttcaggcttc gttggcggag
     3781 cattgattca ctgttgattt tattgagaaa tcggctgcgg attctaacac gaacccgcag
     3841 ccgtcacggc ggcatgttga gatcgatcaa cggctcaatc ggcctgcttg cgcaggaagg
     3901 cggggatctc gtagtggtcg agtccgccgc tggccatcgc atcaaccttg gcaaccgcgc
     3961 gggcattgcg gaagactgct ggtgtcgcgg ccaactcgtc gtagttgccc gacgccaccg
     4021 cgtcgtaggt gccggtccgt gcgttcgaca ccgaatcgct gtcgaccacg gtcagcttgc
     4081 gtgcgccttc ggcgcggccg agtcccgtcg ccaccacggt gacgcgcagc tcgtcctcca
     4141 tcccttcctc gtagacagcg ccgaagatga tgtgggcgtc gtcggcggcg aaggcgcgga
     4201 ccgtgctcat cacctcgttg acttccttca tcttcaggtt tcggctcgag gtgatgttga
     4261 ccaacacgcc gcgcgcgccc gtcagattga ccccttcgag cagcggactg gcgaccgcct
     4321 tttccgcggc gattcgcgcc cggtccatgc cggaggcggc ggccgaaccc atcatcgcca
     4381 tgcccatctc gcccatcacc gtccgcacgt cttcgaagtc gacgttgacc agacccggat
     4441 aggtgatgat ctcggcaatc ccgccgaccg cgttgcgcag cacgtcatca gcggcacgga
     4501 agcattcgtc gacggctgcg tcttcgccga gcacttccat cagcttgtcg ttgaggatga
     4561 cgatcagcga atcaacatgc tgcgtcagct cggcaatgcc tgcctcggcg atcttgattc
     4621 gcttgccctc gaaggagaac ggcttggtca ccacggcgac ggtcaggatg cccatctctc
     4681 gtgccacctc ggcaacgatt ggcgcagcgc cggtgccggt accaccgccc atgccggcag
     4741 taatgaagac catgttggcc ccggtcagcg cggcggcgat ctgctcccgc tgctcgatgg
     4801 ccgatacccg tccggcctcg ggtttggcgc cggcgccgag gccgctcttg ccgagttgca
     4861 gcttgacgtg cgccagactg cgaccgagtg cctgcgcgtc ggtgttggcc gcgagaaact
     4921 cgacgccctg cacgccttcg cgtgccatgt ggtcgacggc gtttccgccg gcgccaccga
     4981 cgccaatgac cttgatgatg gtgctgccac tatccttctc gatgatctcg aacatgcttg
     5041 cctccttcta cgacaaaacg ttaaacgtca aaaattcttt tcaaaccacg cccgcatccg
     5101 ctgcaagacc tgccgcaggt ttcttgcctc cctgactttc aaaccacgcc ggcgctgcga
     5161 ctgcgcttcg agaagcaagc cgtatgccgt tgccaggcgc ggcgtccgca ccacgtcggc
     5221 gagtccgcct tcgtaacgcg gcgcgccgat tcgcaccggc atgtggaata tctcttcgcc
     5281 gagatcgacc atccccggca tcaccgcagc accaccggtc agcacgatcc ccgatgacag
     5341 cagctcctcg aagcccgagc ggcgaatctc cgcctggatc agctcgaaca attcctcgac
     5401 gcgcggctgg atcacgtcgg ccagcgcccg cttggacagg cggcgcggcg gccggtcgcc
     5461 gactccggca acctcgatgt tttcttcggc atcggcgaga tcggcgagcg ccacgccgtg
     5521 gcggcgcttg atctcctcag cctcctgggt cggcgtacgc agcgccattg cgatgtcatt
     5581 ggtgatctgg tcgccggcaa tcggaatcac cgcggtgtgg cggatcgcgc cctgcgtgaa
     5641 gatcgccaga tcggtcgtgc cgccgccgat atcgacgagg cagacgccaa ggtccttctc
     5701 gtcgtcgatc ag
//
LOCUS       DATDAA010000165         2631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_397087,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000165 DATDAA010000000
VERSION     DATDAA010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397087"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="VIS73_05455"
     CDS             <1..126
                     /locus_tag="VIS73_05455"
                     /inference="COORDINATES: protein motif:HMM:NF023095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HEY5762631.1"
                     /translation="LLAGNWLYLLLLACPLMHLFHGHRGHGGHGAPSVKPVDKKE"
     gene            131..2494
                     /locus_tag="VIS73_05460"
     CDS             131..2494
                     /locus_tag="VIS73_05460"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY5762632.1"
                     /translation="MDMPSSHDHGGHEHRHSASGAKADGLKDPVCGMTVTEQSAHRAE
                     HAGRPYYFCGAKCLSKFAADPARYAGDSVVSPDIGAAAQPDGTIYTCPMHPEIRQDHP
                     GDCPKCGMSLEPELPTLEEQENPELIDFTRRFWWTLPLTVMVTVLAMTGHRMGWFEAR
                     TQPWVELLLTLPVALWAGGPFYIRGVQSLINRSPNMWTLIGLGTSVAFVYSVVATVAP
                     QIFPATFVIDGQVAVYFEAAAVIISLTLLGQILELKARSQTSAAIRSLLGLAPKTARR
                     INADGSEEDVPLSNVHVGDQLRVRPGEKVPVDGVVVEGSSALDESMLTGEPLPVTKRP
                     GDKLIGATMNTSGALVMRSEHVGSATVLSQIVQMVAQAQRSRAPMQRMADVVAGYFVV
                     TVVAIAAVTFLAWGLFGPEQGWVFGLINAVAVLIIACPCALGLATPMSVMVATGKGAT
                     GGVLFRDAAAIENLRKVDTLIVDKTGTLTEGRPAFEKAVSAQGFTDVEVLRLAASLDQ
                     GSEHPLAAAIVAAARAQDLALAKPEQFDSESGIGVRGNVDGKALALGNTTLMEQLGVS
                     VAALSAQAEELRSRGASVMHLAVDGKLAGLLSVSDPVKATTPDALAALRKSGLRIVMA
                     TGDGLTTAHAVGTRLGIDEVHGEVKPADKLTLVERLQAEGRIVAMAGDGINDAPALAK
                     ADVGIAMGTGTDVAMNSAQLTLVKGDLRGIATARALSEATVANMKQNLGFAFIYNALG
                     VPLAAGVLFPFTGWLLSPLIAALAMSFSSASVITNALRLRAAKLVQD"
BASE COUNT          444 a          878 c          879 g          430 t
ORIGIN      
        1 cttctcgccg gcaactggct ctacctgttg cttctggcct gcccgctgat gcacctgttc
       61 catggccatc gcggacatgg cggtcacggt gcgccgtcag tcaagcccgt cgataagaag
      121 gaatgacgca atggatatgc cttcgagcca cgatcacggc ggccacgagc atcgccactc
      181 tgcgtcagga gcgaaggccg acggcctcaa ggacccggta tgcggcatga ccgtcactga
      241 gcagtccgcg catcgcgccg agcatgcagg gcgaccctac tatttctgcg gcgcaaagtg
      301 cctgagcaag ttcgctgcag acccggcgcg gtacgccggc gacagcgtcg tgtcgcctga
      361 catcggcgct gcggcccaac ccgacggcac gatctacacc tgccccatgc acccggaaat
      421 ccgccaggac cacccgggcg attgccccaa gtgcggcatg tccctggagc cggaactgcc
      481 gacgctcgaa gagcaagaga acccggaact gatcgacttc acgcgtcgct tctggtggac
      541 tctgccgctc accgtgatgg tgacggtgtt ggcgatgacc ggtcatcgga tgggatggtt
      601 cgaggcgcga acgcagccat gggtcgaact cctgctgacg ctacccgttg cgctgtgggc
      661 tggcgggcct ttctacatac gcggcgtgca gtcgttgatc aatcgcagcc ccaacatgtg
      721 gacgctgatc ggcctgggca ccagcgtcgc gttcgtctac agcgtcgtgg cgaccgtggc
      781 gccgcagatc ttcccggcca ccttcgtcat cgacggtcag gtggcggtgt acttcgaggc
      841 cgccgccgtc atcatctcgc tgaccctgct cggtcagatc ctcgagttga aggcgcgctc
      901 gcagacatca gcggcaatca ggtctctgct cggtctcgcg cccaagacgg cgcgccgcat
      961 caatgcggac ggcagcgagg aggacgtgcc gctttcgaac gtgcacgtgg gagatcagtt
     1021 gcgggtgcgc ccgggcgaaa aagtgccggt cgatggtgtc gtcgtcgagg gcagtagcgc
     1081 gctcgacgaa tcgatgctca cgggcgaacc gttgccggtg accaagcgtc ctggcgacaa
     1141 actgatcggc gcgacgatga acacaagcgg cgcgctcgtg atgcgctcgg agcacgtggg
     1201 ctcggccacc gtgctctcgc aaatcgtgca gatggtggcg caggcgcagc gctcgcgcgc
     1261 gccgatgcag cgcatggccg acgtcgttgc gggctacttc gtcgtcaccg tagtggcgat
     1321 cgccgcggtg accttcttgg cttggggcct gttcggcccc gaacagggtt gggtcttcgg
     1381 cctgatcaac gcggtggccg tgctgatcat cgcctgcccc tgcgcgctcg gcttggccac
     1441 gccgatgtcg gtcatggtcg ccaccggcaa gggtgcgacg ggcggcgtac tcttccgcga
     1501 cgctgcggcg atcgagaacc tgcgcaaggt cgatacgctg attgtcgaca agaccggcac
     1561 gctcaccgag ggacgaccgg ccttcgagaa agcagtatcc gcgcaaggct tcaccgacgt
     1621 cgaagtgctg cgcctcgccg ccagcctgga ccagggcagc gaacacccgc tggccgccgc
     1681 cattgtcgcg gcggcgagag cgcaagacct ggcactggca aagcccgagc agttcgattc
     1741 cgaatcaggc atcggcgtgc gtggcaacgt cgacggcaag gcgctggcgc tgggcaacac
     1801 gaccctgatg gagcaactcg gcgtgtcggt cgccgcgttg agcgctcaag ccgaggaact
     1861 gcgcagccgg ggcgccagcg tcatgcactt ggcagtcgac ggcaagctgg ccgggctgtt
     1921 gtcggtttcc gacccggtca aggcgacgac gcccgacgca cttgccgcgc tgcgcaagtc
     1981 aggcctgcgc atcgtaatgg ccaccggcga cggcctcaca acggcacacg ccgtcggcac
     2041 acgcctgggc atcgacgagg tgcatggcga agtcaaaccc gccgacaagc tgacgttggt
     2101 cgaacggcta caggccgagg gtcgcatcgt cgcaatggcc ggcgacggaa tcaacgacgc
     2161 cccggcactc gccaaggccg atgtcggcat cgcgatgggc accggcaccg acgtggcgat
     2221 gaacagcgcg cagctgacgc tggtcaaagg cgacctgcgg ggcatcgcga ccgcgcgggc
     2281 cctgtcggag gccaccgtgg ccaacatgaa gcagaacctc ggcttcgcct tcatctacaa
     2341 cgccctcggc gtgccgctgg ccgccggtgt gctgttcccc ttcaccggct ggctgctgtc
     2401 gccgctgatc gctgcgctgg caatgagctt tagctcggcc tcggtgatca ccaacgcgct
     2461 tcgcctgcgc gccgccaagc tcgtgcagga ctgaacgccg ggcgacggga ctttcgcggc
     2521 ggtgcgcggc tatgcgctgg cggcaccggg gcaggagccg tgactgccgc cgctggtcca
     2581 gctgctgtcg catcacctgc actgcgtgat gcacagcagg ccgatcgccg c
//
LOCUS       DATDAA010000166         2144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_362254,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000166 DATDAA010000000
VERSION     DATDAA010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362254"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1212
                     /locus_tag="VIS73_05465"
     CDS             241..1212
                     /locus_tag="VIS73_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY5762633.1"
                     /translation="MLLMIIAAGLAMPSFAQEADEPGSEDHPMVSRYAGSYIDGYQVK
                     DYDGFRLPLGPAVWNEDRVRVPEAQADLEGRITRILYRGPRERSTLEILRNYRSALEA
                     AGFETLFACSSDCGNNFAYLLYGPTETRIRTTANSGSAFDIPQDLRYLAARLASGDRV
                     VHVSLLTAFDNGFGALSKQPVTLLEVVESESMDTGMVTVDAEAIGKGIDANGHMAIYG
                     VQFDTDSASIKPESAPVLAEIARLLDSRPGLNILVVGHTDNQGDFEYNVGLSSRRANA
                     VARYLAETHAIDATRLRAEGVGFLAPVASNDTPEGRALNRRVELVKQ"
     gene            1229..>2144
                     /locus_tag="VIS73_05470"
     CDS             1229..>2144
                     /locus_tag="VIS73_05470"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5762634.1"
                     /translation="MRERDARVQAVTEPKKRTACETLSGLHLLLTYECNYECSHCFVW
                     GGPSQTGTMTPEIIERILAEARALGSIEWIYFEGGEPFLYYDTLVRGVRRAAADGFRV
                     GIVSNAYWASSDDEAEARLKPLAGLIDDLSVSSDAYHGSEDGESRAEVARRAARKLGM
                     PVDFISVAAPEATDVAGAAGKLPAGESAVLFRGRAAEKLASKVEPKPWERFDRCPWED
                     LRTPERVHVDPFGNLHICQGISIGNLFERPLPEIMREYDADAHPIVGPLLSGGPAEIA
                     RRYGLPHEDGYADHCHLCYELRRALRGRF"
BASE COUNT          395 a          700 c          707 g          342 t
ORIGIN      
        1 cgaggtcggc ccaagcccat ggaacccaca tgtccgacac accggtcgtc agcaggtaat
       61 agaagaccag gaagaccttc agcgagacga cgaccgggtt tttgcggcgg gcaacgaaca
      121 cccgtggcga tacgcccgga cttcgaggag atccgcggcg ccgccggtct tggcgcgctg
      181 gtttatactt cgatacagcg ggagcctata cgcggaggga gtaaagtggt ccggaacagc
      241 atgctgttga tgattatcgc cgccggcctg gccatgcctt ccttcgccca ggaggcggat
      301 gaaccgggca gcgaggatca tccgatggtg agccgctacg cgggatcgta catcgacggc
      361 taccaggtga aagactacga cggcttcagg ctgccgctcg gcccggcggt gtggaacgag
      421 gatcgcgtca gggtgcccga ggcgcaggcg gacctagaag gccggatcac gcgaatcctt
      481 taccgcgggc cccgggagcg ttctaccctg gagatccttc gcaattaccg atcggcgctg
      541 gaggcggccg gcttcgaaac cctgttcgcc tgcagcagcg attgcggcaa caacttcgcc
      601 taccttcttt acggcccgac ggaaacgcgc atccggacca cggccaattc cgggagcgcc
      661 ttcgacatcc cgcaggacct gcgttacctg gccgcccggc ttgcatccgg tgaccgggtc
      721 gttcacgtgt ccctgctcac cgcgttcgac aacggcttcg gcgcgctcag caagcaaccg
      781 gtcacgctgc tcgaagtcgt cgagtcagag tcgatggaca cgggcatggt gactgtcgac
      841 gcggaggcga tcggcaaggg catcgacgcc aacgggcaca tggctatata cggcgtgcag
      901 ttcgatacgg acagcgcctc gatcaagccg gaatcggcgc ccgtgctggc ggagatcgcg
      961 cggctgctcg acagccgtcc cggactgaat atcctggtag tagggcatac cgataaccag
     1021 ggggacttcg agtacaatgt cggcctgtcc agccggcgcg ccaacgccgt agcgcgctac
     1081 ctggctgaaa cccatgccat tgacgccacc cgcttgcgcg cggagggcgt cggtttcctg
     1141 gcgccggtgg cgagcaacga tacgcccgag gggcgggcgc taaaccggcg cgtggagctc
     1201 gtcaaacagt gaatggccgc cactttgaat gcgagaacgt gatgcacgag ttcaggccgt
     1261 gacagaaccg aaaaaaagaa ctgcctgcga aacgctctcg ggtctgcacc tgctgctgac
     1321 ttacgagtgc aactacgagt gcagccactg cttcgtatgg ggcgggccgt cgcagaccgg
     1381 cacgatgacc ccggaaatca tcgagcgcat tctcgccgag gccagggcgc tcggcagcat
     1441 cgagtggatc tacttcgaag gcggcgagcc ttttctctat tacgacacgc tcgtcagggg
     1501 cgtgcgccgc gccgcggctg atggcttcag ggtcggcatc gtcagcaacg cctactgggc
     1561 aagctcggat gacgaggccg aagcaaggct gaaaccgctc gcgggcctaa tcgacgacct
     1621 gtccgtcagc agcgatgctt atcatggcag cgaggacggc gaatcccgtg ccgaggtcgc
     1681 gcggcgcgcg gcgcgcaaac tcggcatgcc ggtcgatttc atcagcgtcg ccgcgcccga
     1741 ggccaccgac gttgcgggcg cggccggcaa gctgccggcc ggcgagtccg ccgtcctgtt
     1801 ccggggccgc gccgccgaaa agctcgcgtc gaaagtcgag ccgaagccct gggagcggtt
     1861 cgaccgttgc ccctgggaag acctgcgcac gccggagcgc gtgcacgtcg atccgttcgg
     1921 caacctgcat atttgccagg gcatctccat cggcaacctg ttcgagcgcc cgctgcccga
     1981 gatcatgcgc gaatacgacg ccgacgcgca cccgatcgtc gggccgctgc tctccggcgg
     2041 gccggccgag atcgcccgcc gttacgggct gccccacgag gacggctacg ccgaccactg
     2101 ccacctgtgc tacgagctgc ggcgggccct gcgcgggcgt ttcc
//
LOCUS       DATDAA010000167         5299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_47505,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000167 DATDAA010000000
VERSION     DATDAA010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47505"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VIS73_05475"
     CDS             <1..374
                     /locus_tag="VIS73_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762635.1"
                     /translation="DPAMGIVGAILVSIWARRLLVDTGKILLDREMDHPVVEEIRAAV
                     ETTPEAGDTHLADLHVWRVGQGAYACALSLVTHNRELTPDAVRRQLAIHAEIVHVTAE
                     IHQCDLPACNGDSPQLGPVTH"
     gene            432..1445
                     /locus_tag="VIS73_05480"
     CDS             432..1445
                     /locus_tag="VIS73_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762636.1"
                     /translation="MTSYRTILLITATLMLAACAVGGERSRGGLAAPGGHAYVDPAVI
                     EALDDAPACCETLADLPYQPLAAHTQFAVALTPESPAFAFDSGKSFFAAYALAGLTRP
                     FTLEIASRIPAAEEGGAPRPLFAPAALILDAEHRVIEQIDSEPSAAICRPGDRGIAYS
                     LRWRSDATPTAASYIVLLSTEQMRRANRKVLCGVVHHGLSPVGEIAVGSYDDRYGMGE
                     EAAISVRAQRHADGGKPAAEPGVLLVGRTAVHYLEPTLVGFSPALEIPFDRIDFAKTS
                     GGAAWQRAGRRLSLGYRPAASETLATIDFEPDCSGTEPVMFADQLAKEIESRIPAAGA
                     TRR"
     gene            complement(1482..2369)
                     /locus_tag="VIS73_05485"
     CDS             complement(1482..2369)
                     /locus_tag="VIS73_05485"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEY5762637.1"
                     /translation="MSPDLSAAIAAAISAAEGEAFTVREHRSVAGGYIARNLCLADGG
                     RRYFVKIQPPETERIEAESDGLAALAKCPQLVIPRVVASGLADGSAFLVLEWLDFDRT
                     GSDTELAEAIAALHAIEFPRYGWHRDNWIGATPQANGWRDDWADFFVERRLRPQIERV
                     GRQGFGDLQRAAAAALAGLSRGLAGYRPRSGLLHGDLWSGNVGFVGGRPALFDPAVYA
                     GDGETDLAMAALFGGFSPRFFSAYRALRPGDSGAAWRRRLYQLYHVLNHVNLFGVAYV
                     AQASDLIRELGRAGADREV"
     gene            complement(2374..3786)
                     /locus_tag="VIS73_05490"
     CDS             complement(2374..3786)
                     /locus_tag="VIS73_05490"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HEY5762638.1"
                     /translation="MIRRQTSLVLRLTVFFALVSGVVLLGLGWLIAHAIDGHFVELDR
                     DTLRGKIHQVHGIVAGADAATLDDRLRQALPATFSGHHDLYAQVIGPDGQVLYRADGV
                     DFPQPLRYAELPDEMVFNWQDGGRRLRGMAVVLPIGDGEQRLRLAVAIDTAHHDRFMA
                     DFRATLAGYVIVAGLLSGLLGWLAARRGLMPLRAMRTRAAAVTARRLDQRMPVDAVPV
                     EVADLAAELNDMLARLEEAFRRLSEFSSDLAHELRTPISNLLTQTEVSLSQPREAGVY
                     RDILASNSEELQRLARMVADMLFLAKAEHSLVLPTRERVDLGTEVRALFEYYEALADE
                     GGVHLQCTGTGEVVGDRLMIRRAIGNLLSNALRYTARGEAVTVSVRPVSGAIELAVEN
                     PGEAIPADRLPHLFDRFFRADRSRHPGDAEGAGLGLAITRAIVAAHGGSVGASSLGGC
                     NRFILRFPAMPAQAPETTPG"
     gene            complement(3783..4463)
                     /locus_tag="VIS73_05495"
     CDS             complement(3783..4463)
                     /locus_tag="VIS73_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HEY5762639.1"
                     /translation="MKLLIVEDQQKTGDYLQQGLREAGFVVDLARDGIDGSHLALVDD
                     YDLIILDVMLPGRDGWQVLKEIRAAGKAAAVLFLTARDEVHDRVKGLELGADDYLVKP
                     FAFSELLARVRTLLRRGRGGNEPTTLQVADLELDLLRRRASRSGTRIDLTAKEFALLE
                     LLMRRHGEVLPRSLIASQVWDMNFDSDTNVIEVAMRRLRSKIDDGYQPKLIQTVRGMG
                     YVLEVRGA"
     gene            4614..5126
                     /locus_tag="VIS73_05500"
     CDS             4614..5126
                     /locus_tag="VIS73_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HEY5762640.1"
                     /translation="MIRSLLTLAAVAALSGNVIAAGDHASGHGHGDHAGGHGHGDHHG
                     KSTAGTPGDPAKVKRTIKITMDDTMRFSPSTISAKRGETLRLQVSNVGKVAHELVIGP
                     KEELMAHAELMRKFPGMEHDEPNMIRLEPGASGDIVWQFDQDGSVDFACLIPGHMEAG
                     MVGTVKVGKK"
BASE COUNT          944 a         1825 c         1722 g          808 t
ORIGIN      
        1 tcgacccggc gatgggaatc gtcggcgcga ttctcgtttc gatctgggcg cgtcgtctgc
       61 tggtcgatac cggcaagatc ctgctcgacc gcgagatgga ccatccggtt gtcgaggaga
      121 tccgcgctgc cgtcgaaacg actccggagg ccggcgacac gcatctggca gacctccacg
      181 tctggcgcgt cggccagggc gcctacgcct gcgcgctgag cctggtcacc cacaatcgcg
      241 aattgacgcc cgatgccgta cgccggcagc tggcgataca cgcggagatc gtccacgtca
      301 cggccgaaat ccaccagtgc gacctgcccg cctgcaatgg cgacagcccc caattgggcc
      361 cagtgaccca ttgagcagcg cgcgttcagc gcctattatg acgtctgctt tgccattggc
      421 ctttcgacgg cgtgacgtct taccgaacaa tactgctgat tacagcgacc ctgatgcttg
      481 ccgcctgcgc ggtcggcggc gaacgctcgc gtggcggact cgcggcaccc ggtggccacg
      541 cctacgtcga tccggcggtc atcgaggcgc ttgacgacgc accggcctgc tgcgaaacgc
      601 tggccgatct gccgtaccag ccgctcgccg cccacacgca gttcgcggtt gccctgacgc
      661 cggaatcgcc ggcgttcgcg ttcgactcgg gcaagagctt tttcgccgcc tacgcgctgg
      721 caggcctgac gaggcctttc accctggaaa tcgccagccg gatacccgcg gccgaagagg
      781 ggggcgcccc gcgcccgctt ttcgccccgg ccgcgttgat tctcgacgct gaacaccggg
      841 tgatcgagca gatcgacagc gaaccatcgg cggcaatttg ccggccagga gatcgcggca
      901 tcgcctattc gttgcgctgg cgctcggatg ccacgccgac cgccgccagc tacatcgtct
      961 tgctgagcac cgagcagatg cgccgagcca atcggaaggt cctgtgtggc gtcgttcatc
     1021 acggcctgag cccggtcggt gagatcgccg tcggcagcta cgacgatcgc tacggcatgg
     1081 gcgaggaagc cgcgatcagc gttcgcgcac agcgccacgc cgatggcggc aagcctgctg
     1141 ccgaaccggg ggtcctgctg gtcggcagga ccgctgtcca ttacctggag ccgacgctgg
     1201 tcggtttcag ccctgcgctc gagatcccct tcgaccgaat cgatttcgcg aagacctccg
     1261 gcggtgccgc ctggcaacgc gccgggcgcc ggctcagcct cggctatcgc cctgctgcct
     1321 cggagacctt ggcaacgatc gatttcgagc ccgactgctc gggcacggag ccagtgatgt
     1381 tcgccgatca gcttgccaag gagatcgagt cgcgcattcc agcggcaggc gcgacgcgcc
     1441 gctgatcgag gagcagcccg cgccacgtgg cccgcagccg atcaaacctc ccggtcggct
     1501 ccggctcgcc ccagttcgcg gatcaaatcg cttgcctgcg cgacataggc cacgccgaac
     1561 aggttgacgt gattgaggac gtggtagagc tggtagagtc ggcggcgcca cgccgcaccg
     1621 ctgtcgcccg gacgcagcgc ccgataggcg gagaagaagc gcggcgagaa tccgccgaac
     1681 agcgcggcca tcgcgagatc ggtctcgcca tcgccggcgt agaccgccgg atcaaacagc
     1741 gccggccggc cgccaacgaa gccgacgttg cccgaccaga ggtcgccatg cagcaatccc
     1801 gaacgcgggc ggtaaccggc aaggccgcgg ctgagccccg cgagtgcagc cgcggcggcg
     1861 cgctgcagat cgccgaatcc ctgtcgcccc acccgttcga tctgcgggcg cagacgacgc
     1921 tcgacaaaga agtccgccca gtcgtcgcgc cagccgttcg cttggggcgt cgcaccgatc
     1981 cagttgtccc gatgccagcc gtagcgcgga aactcgatcg catgcagcgc ggcaatcgct
     2041 tcggccaact ctgtgtcgct gcccgttctg tcgaaatcga gccattcgag aaccaggaac
     2101 gccgacccgt cggcgaggcc gctggcgacg acgcgcggga tgaccagttg cgggcatttc
     2161 gccagcgcgg ccagaccatc gctttcggcc tcgatccgct ctgtttcggg cggctgaatc
     2221 ttgacgaagt agcgccgccc gccgtcggca aggcacaggt tccgtgcgat gtagccgccg
     2281 gcgaccgagc gatgctcgcg gaccgtgaac gcctcgccct cggcagcgct gatggccgcg
     2341 gcaatggcag cggacaggtc gggagacacc tgcttagccg ggcgtggttt caggcgcctg
     2401 cgctggcatc gccggaaagc gcaagatgaa gcggttgcat ccgcccagag agctggcgcc
     2461 gacgctgccg ccatgcgcgg caacgatcgc ccgcgtgatc gccagcccca gcccggcgcc
     2521 ttcggcatcg ccggggtgcc gcgaacgatc ggcgcggaag aagcgatcga agagatgtgg
     2581 caggcggtcg gcgggaatcg cctcgccagg gttctcgacc gccagctcga ttgccccgct
     2641 gaccgggcgc acgctgacgg tgaccgcctc gccacgcgcc gtgtagcgca gcgcgttgga
     2701 aagcaggttg ccgatggcgc ggcggatcat caggcgatcg ccgacgactt cgccggttcc
     2761 ggtgcattgc aggtggacgc cgccctcgtc ggccagcgct tcgtagtact cgaacagcgc
     2821 gcgcacctcg gtcccgaggt cgacgcgctc ccgcgtgggc agcaccaggc tgtgctctgc
     2881 ctttgccaaa aagagcatgt cggcaaccat gcgcgccaga cgctgcaact cctcggaatt
     2941 ggaggcgagg atgtcgcggt agacgccggc ctcgcgcggt tgcgacagcg agacctcggt
     3001 ctgcgtcagc aggttgctga tcggcgtccg cagctcgtgg gcgagatcgg acgaaaactc
     3061 cgagagacgg cgaaatgcct cttccagccg cgccaacatg tcgtttagtt cggccgccag
     3121 atcggccacc tcgaccggga cggcgtcgac cggcatgcgc tggtcgagcc gccgagcggt
     3181 gactgccgcg gcgcgcgtgc gcatcgcccg cagcggcatc agaccacggc gtgccgccag
     3241 ccagccgagc aggccgctaa gcaggccggc gacgatcacg tagccggcca gcgtggcacg
     3301 gaagtcggcc atgaaccggt cgtgatgcgc ggtgtcgatg gcaaccgcca gccgcaggcg
     3361 ctgctccccg tcgccgatgg gcagcacgac ggccatgccg cgcaggcgcc gtccgccgtc
     3421 ctgccagttg aacaccatct cgtcgggcag ctcggcgtag cgaagcggct gcggaaagtc
     3481 gacaccgtcg gcgcggtaga gcacctggcc atcggggcca atcacctgcg catagaggtc
     3541 gtggtggccg ctgaaggttg ccggcagcgc ctgccgcagt cggtcgtcca gcgtcgcggc
     3601 atcggcgccg gcaacaatgc cgtgcacctg atggatcttg ccgcgaagcg tgtcgcgatc
     3661 gagttcgacg aagtgaccgt cgatcgcgtg cgcgatcagc cagcccagcc ccagcaggac
     3721 gacgcccgaa accagggcaa agaagaccgt cagccgcagg acaagcgacg tctgccgccg
     3781 aatcatgcgc cgcgcacttc gagcacgtag cccatgccgc gcacggtctg gatcagcttc
     3841 ggctggtaac cgtcgtcgat cttgctgcgc agccgccgca tcgcgacttc gatcacgttg
     3901 gtgtcgctgt cgaagttcat gtcccagacc tgcgaggcga tcagcgaacg cggcagcact
     3961 tcgccgtgcc gccgcatcaa cagctcgagc agcgcgaatt cctttgcggt caggtcgatt
     4021 cgcgtgccgc tgcggctggc gcgccggcgg agcagatcga gttcgaggtc ggccacctgc
     4081 agcgtggtcg gctcattgcc gccacggccg cggcgcagca gcgtgcgcac gcgcgccagt
     4141 agttcggaga aagcgaaggg cttgaccagg tagtcgtcgg ccccgagttc gaggccctta
     4201 acccggtcgt gaacctcgtc gcgcgcggtc agaaacagca ccgccgcagc cttgccggcg
     4261 gcgcggatct ccttcagcac ctgccagccg tcgcggcccg gcagcatgac gtcaaggatg
     4321 atcaggtcgt agtcgtcgac cagcgccaag tggctgccgt cgatgccgtc acgcgccaga
     4381 tcaacgacaa aaccagcttc gcgcaggccc tgctgcaggt agtcgccggt cttttgctgg
     4441 tcttcgacga tcaggagctt catcgacaaa tcctagattg ggaacggcca tagtgtgcca
     4501 agaaccactg gccacccggc atttgattac agaaatgtaa tcatcgtgtc agctagcagt
     4561 cctcggccgc ttgccagaat ggccgccatc gtcaccaccc ggagcaccaa gacatgattc
     4621 gcagcctgct tacgctggcg gcagtggccg ccctgtccgg caacgtcatc gccgccggcg
     4681 accacgccag tggccacggt cacggcgacc acgccggtgg ccacggtcac ggcgaccacc
     4741 acggaaagtc gactgcggga acacccggcg atccggcaaa ggtcaagcgc acgatcaaga
     4801 tcaccatgga cgacacgatg cgcttttcac catcgacgat ctccgccaag cgtggcgaaa
     4861 cgctgcgcct gcaggtcagc aacgtcggca aggtcgccca cgaactggtg atcggcccga
     4921 aggaggaact gatggcccac gccgagctca tgcgcaagtt ccctggcatg gagcacgacg
     4981 agccaaacat gatccgcctc gaacccggcg ccagcggcga catcgtctgg cagttcgacc
     5041 aggacggcag cgtcgacttc gcctgcctga tccccggcca catggaggcg ggaatggtcg
     5101 gaacggtcaa ggtcggcaag aagtaagaca ccagattctc gcgccctgca acaatacaaa
     5161 ctccaggaga acaccatgaa agcgtctcac ctcgccgccg ccctgctcgc gctgggcctt
     5221 gcgcaaccaa tcttcgccga tgacaagatg atgaagccgg gccacgcgca ggccatggaa
     5281 atgtcggaag gaaccgtca
//
LOCUS       DATDAA010000168         3179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_222761,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000168 DATDAA010000000
VERSION     DATDAA010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222761"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VIS73_05505"
     CDS             <1..285
                     /locus_tag="VIS73_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY5762641.1"
                     /translation="NALTKQYKKLFDMEGVELELRPAALNAIAKRALARKTGARGLRS
                     ILESILLDTMYDLPSLENVAKVVIDENNVNGGGQPIMIYADQQKVAGGKK"
     gene            399..2810
                     /gene="lon"
                     /locus_tag="VIS73_05510"
     CDS             399..2810
                     /gene="lon"
                     /locus_tag="VIS73_05510"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY5762642.1"
                     /translation="MSDQKTILTEPGDLPLLPLRDVVVFPHMVIPLFVGRPKSIRALD
                     TAMESDKAIMLVAQKSAAKDDPGAEDLHSIGCVANILQMLKLPDGTVKVLVEGTQRAR
                     IADVTEDDGLFRANVLPIAPEESGTHEAEAMRRAVISQFDQYVKLNKKIPPEILTSIA
                     GIEEAGRLADTIAAHLPLKLEQKQEVLEIFDVKARLERLLSQLETEIDIMQVEKRIRG
                     RVKRQMEKSQREYYLNEQVKAIQKELGEGEEGADLDELEKKIRAAGMSKEALKKAESE
                     LKKLRMMSPMSAEATVVRNYIDTLVALPWKKKTRISKDLSEAEKVLDKDHYGLERVKE
                     RIVEYLAVQQRVDKVKAPILCLVGPPGVGKTSLGQSIAKSTNRKFIRMALGGVRDEAE
                     IRGHRRTYIGSMPGKILQNMSKVGVRNPLFLLDEVDKLGMDFRGDPSSALLEVLDPEQ
                     NHTFQDHYIEVDFDLSDVMFVATANTLNIPAALLDRMEVIRLSGYTEEEKVNIAQRYL
                     LPKQMKNNGLKKTELSVTEAAIRDIIRYYTREAGVRSLERDLSKICRKVVKTLVLKKR
                     SSRIVVNEKVLDKYLGVRRYSFGMAEKENQVGQVTGLAWTEVGGELLTVEAVALPGKG
                     KTITTGKLGEVMQESIQAAISVVRRRSRTLGISEDFYQKNDIHIHLPEGAIPKDGPSA
                     GVAIATALVSILTGIPVRCDVAMTGEITLRGEVLPIGGLKEKLLAAVRGGIKTALIPD
                     ENVKDLAEIPDVIKNRIEILPVKWFDKVIELALERKPEPLSVPEPEATPVAGGEPPPT
                     GLMKH"
     gene            2937..>3179
                     /locus_tag="VIS73_05515"
     CDS             2937..>3179
                     /locus_tag="VIS73_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762643.1"
                     /translation="MGFGELVGLSSVRQKGLDCRRHLFGLIVLQIVSAVGKRLLALIR
                     EAAEATRELFGLPAEAVDNETLCREQIQNGCADPAPA"
BASE COUNT          656 a          976 c         1024 g          523 t
ORIGIN      
        1 aacgcgctga ccaagcagta caagaagctg ttcgacatgg aaggcgtcga actcgagctt
       61 aggccggccg cgctcaatgc catcgccaag cgtgcgctgg cacgcaagac cggggcgcgt
      121 ggtctgcgct cgattctcga aagcatcctg ctcgacacga tgtacgacct gccgagcctg
      181 gagaacgttg ccaaggtggt catcgacgaa aacaacgtca atggcggcgg tcagccgatc
      241 atgatctatg ccgaccagca gaaggttgcc ggcggcaaga agtagtcggg cacgggcatc
      301 gatccccggc ccggatacct cccgccggcc gccccgactt gaaatttccc tgaagcgccc
      361 ccaactgggc acttcgtccc cattccggaa ggccaacgat gtccgatcaa aagaccattc
      421 tcaccgaacc gggtgacctg ccgctcctgc cgctgcggga cgtggtcgtc ttcccacaca
      481 tggtgattcc gctgttcgtc ggtcggccga aatcgatccg ggcgctcgat actgcgatgg
      541 agtccgacaa ggcgatcatg ctggtggccc agaagtcggc tgccaaggac gatccgggtg
      601 ccgaggacct gcactcgatc ggctgcgttg ccaacatcct gcagatgctc aagctgcccg
      661 atggcaccgt caaggtgctg gtcgagggca ctcagcgcgc gcgtatcgcc gacgtcactg
      721 aggacgatgg cctgttccgc gcgaacgtgc tgccgattgc ccccgaggaa agcggcacgc
      781 acgaagccga ggcgatgcgt cgcgcggtga ttagccagtt cgaccagtac gtcaagctca
      841 acaagaagat cccgccggag atcctgacct cgatcgccgg catcgaagag gccgggcgtc
      901 tcgccgacac catcgccgcc cacctgccgc tcaagctcga gcagaagcag gaagtgctcg
      961 agatcttcga cgtcaaggcg cggctcgagc ggctgctgag ccagctcgag accgaaatcg
     1021 acatcatgca ggtcgaaaag cgcatccgtg gccgcgtcaa gcggcagatg gagaagagcc
     1081 agcgcgagta ctacctcaac gaacaggtca aggcgatcca gaaggaactc ggcgaaggcg
     1141 aagagggcgc tgacctcgac gagctcgaga agaagatccg cgctgcgggc atgagcaagg
     1201 aggcgctgaa gaaggccgag tccgagctca agaagctgcg catgatgagt ccgatgtcgg
     1261 ccgaggcgac cgtggtccgc aactacatcg acacgctggt cgcgctgccg tggaagaaga
     1321 agacgcggat cagcaaggat ctctccgaag ccgaaaaggt actcgacaag gaccactatg
     1381 gcctcgaacg cgtcaaggaa cgcatcgtcg aataccttgc cgtccagcaa cgtgtcgaca
     1441 aggttaaggc gcccattctt tgccttgtcg gccccccggg tgtcggcaag acctcgctcg
     1501 gccagtcgat cgccaagtcg accaaccgca agttcatccg catggcgttg ggcggcgtgc
     1561 gcgacgaggc cgagattcgc ggccaccgac gcacctacat cggctcgatg ccgggcaaga
     1621 tcctgcagaa catgtcgaag gtgggcgtgc gcaatccgct cttcctgctc gacgaggttg
     1681 acaagctggg catggatttt cgcggcgatc cgtcgtcggc gctgctagaa gtccttgatc
     1741 ccgagcagaa ccacaccttc caggatcact acatcgaggt cgatttcgat ctgtcggacg
     1801 tcatgttcgt ggccacggcc aatacgctga acattccggc ggcgctgctc gaccgcatgg
     1861 aagtgatccg cctctcgggt tacaccgagg aggaaaaggt caacatcgcc cagcgatacc
     1921 tgctgcccaa gcagatgaag aacaacggcc tcaagaagac cgagttgtcg gtcaccgagg
     1981 cggcgatccg cgacatcatc cgctactaca cgcgagaggc cggtgtccgt tcgctcgagc
     2041 gcgacctgtc gaagatctgc cgcaaggtgg tgaagactct ggtgctgaag aagcgctcgt
     2101 cgcgcatcgt cgttaacgag aaggtgctcg acaagtacct gggcgtgcgc cgctactcct
     2161 tcggcatggc cgaaaaggaa aaccaggttg gtcaggtgac cgggctggcg tggaccgagg
     2221 tcggcggcga gttgctgacc gtcgaggcgg tggcgctgcc aggcaagggc aagaccatca
     2281 ccaccggcaa gctcggcgag gtcatgcagg agtcgatcca ggcggcgatc tcggtggtcc
     2341 gccgccgttc gcggacgctt ggcatcagcg aggacttcta ccagaagaac gacatccaca
     2401 ttcacctgcc cgaaggcgcg attcccaagg acggtccgtc ggccggtgtg gcgatcgcta
     2461 ccgcgctggt gtcgatcctg accggcattc ctgtgcgttg cgatgtggcg atgacgggcg
     2521 agatcactct gcgcggcgaa gtgctgccga tcggcgggct caaggaaaag ctcctcgcgg
     2581 cggtgcgcgg cggcatcaag accgcgctga tccccgacga gaacgtcaag gaccttgccg
     2641 agattcccga cgtgatcaag aatcgcatcg aaattctgcc cgtgaaatgg ttcgacaagg
     2701 tcatcgagct ggcactggag cgaaagcctg agccgttgtc cgtgccggag cccgaggcga
     2761 cgccagtggc gggcggcgaa ccgccgccga ccggactgat gaagcactag aaggatcatg
     2821 aacaaggccg gcgatgtccg gccttgttct ttcttgccgg cgggtcatca gtctgccgat
     2881 tagtcacccc gtggcggtgc aggcgccgtt agccgggccg cggcgtgagg ggtggcgtgg
     2941 gcttcggcga gctggttggc ctatcaagcg tgcgccagaa aggcctcgat tgccgccgcc
     3001 acctgttcgg gttgatcgtg ctgcagattg tgtccgcagt cggcaagcgt ctgctcgcac
     3061 tgattcggga agcagccgag gcgacgcgcg agctcttcgg gctgccggcc gaagcggtcg
     3121 ataacgaaac cctctgccgc gagcagattc agaacgggtg cgctgatccg gcgccagcc
//
LOCUS       DATDAA010000169         6310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_47642,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000169 DATDAA010000000
VERSION     DATDAA010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47642"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="VIS73_05520"
     CDS             <1..1379
                     /locus_tag="VIS73_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY5762644.1"
                     /translation="TVPALDGKLLDEARFNKATRYQTAIDEPAVPLDNQNLFVAFVDV
                     VQKAGKGYVVYEWPKAKAGGGTTTELYEKLSYVKGFEPWGWLVGSGIYIDDVDAQFRS
                     EALRLGAVSIVVVGFLLGISALVGKSILRQLGGEPAYAASITRQIAEGDLRQTVTVAG
                     GSDSMLGAVKAMQERLAQMFRDISSAADRLSSNAEEVASAARESSEAGQHQAQATAAM
                     AASLEEVTVSVNEVSAIARANEESSRKVAAVAESGQSEVSAASEHIARVATTVERASH
                     QVQALVRRSEEIGGIANVIKEIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLA
                     ERTAKATTEIASVIGTVQVETRAAVQEMEEALPLVQESVGLTQKATVSLDSIRNESAD
                     SLTHVQEVAHATREQATASNDIARNVEQIATMADEVNAAMANTAEHAKEMDSIAQALR
                     EKVSFFKV"
     gene            1464..2438
                     /gene="miaA"
                     /locus_tag="VIS73_05525"
     CDS             1464..2438
                     /gene="miaA"
                     /locus_tag="VIS73_05525"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041646634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY5762645.1"
                     /translation="MPAINETAAAHRPPAILLIGPTASGKSGAALAIAEVLPVEVIAV
                     DSATVFRDMDIGTAKPDAATRARTPHHLVDIVSPEEHYSAGRFRSDALAAMAEIRARG
                     KVPLLVGGTMLYVKALTEGLSKLPTGDAALRAAIDAEAAERGWPALHAELAEIDPVAA
                     EKIAPTNPQRIQRALEVYRASGRPISSFWTEGRPGAAAADFLTIALVPGDRAVLHQRI
                     AQRFRQMLADGLVDEVQSLKRKYRLDASLPSMRCVGYRQVWDMLDGAMPAAELEDRGI
                     FATRQLAKRQLTWLRSMDGLHEIDCLAANAKTRALAAVQTFLGRHAAA"
     gene            2540..4039
                     /locus_tag="VIS73_05530"
     CDS             2540..4039
                     /locus_tag="VIS73_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEY5762646.1"
                     /translation="MLFNSNTFLFLFLPVVLAGFFLIGRWYREAAAFWLFASSVAFYG
                     MWSAAYIALLIGSIVFNYTVGVNLSRLVATGRATAATWLIRGGVAGNLLLLGYYKYAN
                     FFVDSAAELAGTHWHMAEIILPIGISFYTFTQIAFLVDCYRGEVRETNPIHYGLFVTY
                     FPHLIAGPVLHHKEMMPQFAEAKTYRPDWENLAVGTTIFAIGLFKKVVIADGIGPFAA
                     PVFNAAAAGQSLTALEAWGGALAFTFQLYFDFSAYSDMAIGLSKLFGVRLPLNFNSPY
                     KAASIIDFWRRWHMTLSRFLRDYLYFALGGNRRGKLRRHLNLFITMLLGGLWHGAGWT
                     FVAWGALHGVYLMINHGWRSLRKRLFGESRGASRLEKIAATALTFLAVVVAWVFFRAA
                     DFQSALAILQAMAGGNGFAGGDFAVFTQAYLSPSRLLGALILCAIITWGFPNSQQLLA
                     EHRPALETVRSPSRIRWQPSARWLAICAATLYVALIEMGKVSEFLYFQF"
     gene            4014..5135
                     /locus_tag="VIS73_05535"
     CDS             4014..5135
                     /locus_tag="VIS73_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762647.1"
                     /translation="MSSSTSSSDRFRRFTLGVLLLAFLPAALFFAIGIWLQPLDGDLT
                     RAGSFSQRHYGWNSTQIVYRTLRYSLGPYDRPYDVVVYGDSFAVAMPSHQWQNRLAEA
                     TGLSVITLNVYTDPLARVLENPVFVATPPKVFVLTLAERHFPEQLAKLSDCAPDATPP
                     QSEMVAAAPANALPAMPTDLPTIADAVETRRQTHWQDLREVKIGYAAKTVFHTLLRAV
                     SKRERTKVVRVSLTRDDLFSNLDPGRTLVYRDDVDKVAQWQAEGLDGLVCRIETLRRR
                     IEANGVTRFVLLVAPDKLTAYEPYLATDDYRGLSRLAELAERLPGTMPRVDTAIAAVI
                     AAGHVDVYLPNNTHWGSAGHLATGEAVARFIEATNVVPR"
     gene            complement(5149..6036)
                     /locus_tag="VIS73_05540"
     CDS             complement(5149..6036)
                     /locus_tag="VIS73_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762648.1"
                     /translation="MNLRAPLAAAFLAALLPAVAIAAGDFRYGIDSDLAHDSNVSRGL
                     ADADTVADRIVTIEGSATRSVLLGPESGLLLRAAARYARYLDLTDLSHFGLAGRAAWR
                     TQRDRSFGSPWYEVAVDGLYLRHADSELRDGFQLSLSGSIGSHLTDRVRVAGGVAFDK
                     RSGGGLGLYDLTGNRLWATLDYRVGLRDVAYARLTRSAGEQVFTAISAASQALLLQSG
                     YTILAADTALADGFGGVATTAYRADATSWNYELGYNLPISRTRTLDFSFGYFVAKTDL
                     GAYQYDGLQARAAYLYRFQ"
     gene            6105..>6310
                     /locus_tag="VIS73_05545"
     CDS             6105..>6310
                     /locus_tag="VIS73_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762649.1"
                     /translation="MSRTKHEPANELDDPRRAFLIRALAAGAFATGAPAAWAQIFGRK
                     PGKLPTGKSIYESVGDVRVDGKPA"
BASE COUNT         1099 a         2221 c         2000 g          990 t
ORIGIN      
        1 cgacggttcc cgcgctggac ggcaagctgc ttgacgaggc gcgcttcaac aaggcaaccc
       61 gctaccagac ggcgatcgac gaaccggccg taccgctcga caaccagaac ctcttcgtcg
      121 ccttcgtcga tgtcgtgcag aaggccggca agggctatgt cgtctacgag tggccgaagg
      181 ccaaggccgg cggtggaacc acgaccgaac tctacgaaaa gctctcctac gtcaagggct
      241 tcgagccctg gggctggctg gtcggctcgg gcatctacat cgacgatgtc gacgcgcagt
      301 tccgcagcga ggcgctgcgc ctgggcgccg tgtcgatcgt cgtagttggc tttctcctcg
      361 gcatctcggc gttggtcggc aagagcatcc tccgtcagct cggcggcgaa ccagcctacg
      421 cggcgagcat tacccggcag atcgccgaag gcgacctgcg acagaccgtc acggttgccg
      481 gcggcagcga cagcatgctc ggcgccgtaa aggcaatgca ggagcgactg gcgcagatgt
      541 tccgcgacat cagcagcgcc gccgaccgac tgtcgagcaa tgccgaagaa gtggcatcgg
      601 ccgcccgcga atcgagcgaa gccggccagc accaggcgca ggcaactgcg gcaatggccg
      661 cctcgctcga agaggtcacc gtcagcgtca acgaggtttc ggctattgcg cgagccaacg
      721 aggaaagctc gcgcaaggtt gccgcggtgg ccgaatcggg ccagagcgag gtttcggcgg
      781 ccagcgagca catcgcccgc gtcgcgacga cggtcgagag ggcatcgcat caggtacagg
      841 cgctggtccg ccgctccgag gaaatcggcg gcatcgccaa cgtcatcaag gagatcgccg
      901 accagaccaa cctgctggcg ctcaacgccg cgatcgaggc ggcgcgtgcc ggcgagcagg
      961 gccgcggctt cgccgtggtc gccgacgagg tacgcaagct cgccgagcga acggccaagg
     1021 cgaccaccga gatcgccagc gtcatcggca cggtgcaggt cgagacccgc gccgccgtgc
     1081 aggaaatgga agaggcgctg ccactcgtgc aggagagcgt cgggctgacg cagaaggcga
     1141 ccgtgagcct cgattcgatc cgcaacgaat cggccgactc gctgacccac gtgcaggaag
     1201 tcgcgcacgc cacccgcgag caggcgactg cctccaacga catcgcccgc aacgtcgagc
     1261 agattgcgac gatggccgac gaggtcaacg cagcgatggc caacactgcc gagcacgcca
     1321 aggaaatgga ttcgatcgcg caggcgctgc gcgagaaggt ttccttcttc aaggtctgat
     1381 gcggcgggcc ggcgacggct gaccgacgag gcgccgctag aatggcggcc tagtccgagt
     1441 cccgccgtcc gtgcccgccg tccgtgcccg ccatcaacga aaccgccgcc gcccaccgcc
     1501 cgcccgcgat cctgctgatc ggcccgaccg ccagcggtaa gagcggcgcg gcactggcga
     1561 tcgccgaagt gctcccggtc gaggtcatcg ccgtcgattc ggccaccgtg ttccgcgaca
     1621 tggacattgg cacggccaag cccgacgccg cgacgcgggc gcgcaccccg caccacctgg
     1681 tcgatatcgt cagccccgag gaacactact cggccggccg ttttcgcagc gacgcgctcg
     1741 ccgcgatggc cgagatccgc gcccgtggca aggttccgct gctggtcggt ggcacgatgc
     1801 tctacgtcaa ggcactgacc gagggcctct ccaagctgcc gaccggcgac gccgcgctgc
     1861 gcgccgcgat tgatgcggag gccgccgaac gcggctggcc ggcgcttcac gccgagctcg
     1921 ccgagatcga ccccgttgcc gcggagaaaa tcgcaccgac caacccgcag cgcatccagc
     1981 gcgcgctcga ggtctaccgc gccagcggcc ggccgatctc gagcttctgg accgaaggcc
     2041 gccccggagc cgccgccgcc gacttcctga cgatcgcgct ggtccctggt gatcgcgctg
     2101 tcctgcacca acggatcgcg cagcgcttcc gccagatgct ggccgacggc ctggtcgacg
     2161 aagtgcaatc cctgaaacgc aagtaccggc tcgatgcctc gctgccctcg atgcgctgcg
     2221 tcggctaccg acaggtctgg gacatgctcg acggcgccat gcccgccgcc gagctcgaag
     2281 accgaggcat cttcgccacg cgccagctcg ccaagcgcca gctcacctgg ctgcgcagca
     2341 tggacggcct gcacgaaatc gattgcctcg cggccaacgc taagacccgg gcactggcgg
     2401 cggtccaaac cttcctgggt cgccacgcgg ccgcctgatc ggcgacaatc gcgccctcgc
     2461 ccatccgagc gccggcgccg cacccacgac ggggtgagag ccggccgcaa caattgcccg
     2521 cgaccgaccc gcatcgcccg tgctgtttaa ctccaacacc tttctcttcc tgttcctgcc
     2581 ggtcgtgctg gccggattct tcctgatcgg ccgctggtat cgcgaagccg cggcgttctg
     2641 gctgttcgcc tcctcggtcg ccttctacgg catgtggagc gccgcctaca tcgcgctgct
     2701 gatcggctcg atcgtcttca actacacggt gggcgtgaac ctgagccggc tggtcgccac
     2761 aggccgggcg acggccgcaa cctggctgat ccgcggcggc gtagccggca acctgctgct
     2821 gctcggttat tacaagtacg ccaacttctt cgtcgacagc gccgccgagc tcgccggcac
     2881 tcactggcac atggcggaga tcatcctgcc gatcggcatc tccttctaca ccttcacgca
     2941 gatcgccttc ctcgtcgact gctatcgcgg cgaagtgcgc gagaccaatc cgatccacta
     3001 cggactgttc gtcacctact tcccgcacct tattgctggc ccggtgctgc accacaagga
     3061 aatgatgccg cagttcgccg aggccaagac ctaccggccc gactgggaaa acctcgctgt
     3121 cggcacgacg atcttcgcca tcggtctatt caagaaagtg gtgatcgccg acggcatcgg
     3181 gccttttgcg gcgccggtgt tcaatgccgc ggcagccggg caatcgctca ccgcgctgga
     3241 agcctggggt ggtgcgctgg ccttcacctt ccagctctac ttcgacttct cggcgtattc
     3301 cgacatggcg atcggactgt cgaagctgtt cggcgtgcgc ctgccgctca acttcaactc
     3361 gccctacaag gcggcgagca tcatcgactt ctggcgccgc tggcacatga cgctgtcgcg
     3421 ctttttgcgc gactacctct acttcgcgct cggcggcaac cgccgcggca agctgcgccg
     3481 acacctgaac ctgttcatca ccatgctgct cggcggcctc tggcatgggg ccggctggac
     3541 cttcgtcgcc tggggcgcgc tgcacggcgt gtacctgatg atcaaccacg gctggcgcag
     3601 cctgcgcaag aggctgttcg gcgaatcgcg cggcgcatcg cgtctggaga agatcgccgc
     3661 gaccgcgctg accttcctcg ccgtcgtcgt cgcctgggtc ttcttccgcg ccgcagactt
     3721 tcagtcggcg ctcgcgatcc tgcaggcgat ggccggtggc aacggtttcg ccggcggcga
     3781 cttcgcggtc ttcacgcagg cctacctctc gccctcacgg ctgctcggcg cactgatcct
     3841 ctgcgcgatc atcacctggg gcttccccaa ctcgcagcag ttactggccg aacaccggcc
     3901 agcgctcgaa accgtccgct cgccaagccg catccgttgg cagcccagcg cgcgctggct
     3961 ggcgatctgc gccgcgaccc tctatgtcgc gctgatcgaa atgggaaaag tcagtgagtt
     4021 cctctacttc cagttctgat cgctttcgcc gcttcacgct cggcgtgctg ctgctggcct
     4081 ttctgccggc ggcgctgttc ttcgccatcg gaatctggct gcagccgctc gacggcgacc
     4141 tgacgcgcgc cggctccttc tcgcagcgcc actacggctg gaacagcacg cagatcgtct
     4201 accgcacact ccggtattcg ctcggaccct acgaccggcc ctacgacgtc gtcgtttacg
     4261 gcgattcctt cgccgtcgcg atgccctcgc accagtggca gaaccgcctc gccgaggcga
     4321 ccggactatc ggtcatcacg ctcaacgtct atacagaccc gctggcgcgc gtgctggaga
     4381 atccggtatt cgtcgccaca ccgccaaagg tcttcgtgct gacgctcgcc gagcggcact
     4441 tcccggaaca gctggccaaa ctcagcgact gcgcgccaga cgcaacgccc ccccagagtg
     4501 aaatggtggc cgccgcgccc gcaaacgcgc tgccggccat gccgaccgat ctaccgacca
     4561 tcgccgatgc cgtcgaaacc cggcgccaga cgcactggca ggacctccgc gaagtgaaga
     4621 tcggctacgc ggcgaagacg gtcttccaca ccctgctgcg tgcggtatcc aagcgcgagc
     4681 ggaccaaggt ggtgcgcgtg tcgctgacgc gcgacgacct cttctccaac ctcgacccag
     4741 gccggacgct ggtctatcgc gacgacgtgg acaaggtcgc gcagtggcag gccgagggcc
     4801 tcgacggcct cgtttgccgc atcgaaaccc tgcgccgccg gatcgaggcc aacggcgtta
     4861 cccgtttcgt tctgctggtg gcgccggaca agttgaccgc ctacgagccc tacctggcga
     4921 ccgacgacta ccgcggcctg agccgcctag ccgaactcgc cgagcgtctg cccggcacga
     4981 tgccgcgcgt tgacacggca atcgccgccg ttatcgccgc cggccacgtc gatgtgtacc
     5041 tgccgaacaa tacgcactgg ggcagcgcgg gccacctcgc gaccggcgaa gcggtcgcga
     5101 ggttcatcga agcaaccaac gttgtaccgc gctgaaggcg gcggctcgct actggaagcg
     5161 atacagatag gcggcgcgcg cctgcaagcc gtcgtactgg taggcgccga ggtcggtctt
     5221 ggcaacgaag tacccgaaac tgaagtcgag cgtgcgcgtg cgcgagatcg gcaggttgta
     5281 gccgagctcg tagttccagc tcgttgcatc ggcccgatag gccgttgtcg cgacgccgcc
     5341 gaaaccgtcg gccagcgcgg tgtcggcagc caggatcgtg tagcccgact gcaacaacaa
     5401 cgcctgcgaa gcggcgctga tcgccgtgaa gacctgctcg ccggccgagc gcgtgaggcg
     5461 cgcgtaggcg acatcgcgca ggccgacgcg gtagtcgagc gtcgcccaca ggcgattgcc
     5521 ggtgagatcg tagagaccca gaccgccacc gctgcgcttg tcgaatgcga cgccgccggc
     5581 gacacgaacg cggtcggtga gatggctgcc gatgctgccg ctcaggctga gctggaaacc
     5641 atcgcgcaac tcgctgtcgg cgtgccgcag gtaaagccca tcgacagcga cttcgtacca
     5701 gggcgagccg aaggaacgat cgcgctgggt gcgccaggca gcgcgaccgg caagtccgaa
     5761 atggctgaga tcggtgaggt cgaggtaacg ggcgtaacgc gccgcggcgc gtaagagcag
     5821 gccggactca gggccgagca gtacgctgcg cgtcgccgag ccctcgatgg tcacaattcg
     5881 gtcggccacc gtatcggcat cggcaaggcc tcgcgagacg ttggaatcat gcgcaagatc
     5941 ggagtcgatg ccgtatcgga aatcgccggc ggcgatggcc acagccggca acagtgccgc
     6001 cagaaaggcc gcagcaagcg gagcgcgcag gttcatctcg attccccaat cggttggctt
     6061 cgcgcaataa tacgtcgcca accccgacac cggcagggca agctatgagc cgaaccaagc
     6121 acgaacccgc aaacgaactc gacgatccgc gccgcgcctt tctgattcgc gcgcttgccg
     6181 ccggtgcctt tgccaccggc gcaccggccg cttgggcaca gatattcggc cgcaagccgg
     6241 gcaagctgcc gactggcaag tcgatctatg agtccgtcgg cgacgtacgg gtagacggta
     6301 agccggccga
//
LOCUS       DATDAA010000170        15184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_187693,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000170 DATDAA010000000
VERSION     DATDAA010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 15184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15184
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187693"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VIS73_05550"
     CDS             <1..476
                     /locus_tag="VIS73_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition family protein"
                     /protein_id="HEY5762650.1"
                     /translation="ETLHHPVAETAKGVLDVINRAGAEAFKIAVGAIPMLVISLVLVA
                     LLKAVGAIDLLTVLLRPLLVLLGIDPALILLTLTKVIAGGTAMMGVMDDMLRSGEASA
                     SLMNGSAGFLIHPLDVAGVAVLISAGRRVAQVWKPAVAGGMVGIAVRTLGHALGS"
     gene            complement(507..2093)
                     /locus_tag="VIS73_05555"
     CDS             complement(507..2093)
                     /locus_tag="VIS73_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY5762651.1"
                     /translation="MELKDIKEFDKAAQKSQPELLRFGLAFLFIVGVMFFTWARGDGS
                     FLLVVAAMIGGYMAMNIGANDVANNVGPAVGSRALTMIGALVIAGVCEAAGALIAGGD
                     VVATIRGGIISQDQIGSADTFVWLMMGALLAGAIWLNIATAIGAPVSTTHSIVGGVLG
                     AGIAAGGWGVVSWGKVSAIAASWVISPVLGGLIAAAFLYLIKRRITYQSDMMDAARRV
                     VPVLVALMAGVFITYLMLKGVRAVVKVSFYSAIFWGVIGGGLVYLFVRPRVAWYSRQT
                     GEAVGDKAKVNRMFTVPLIFAAALLSFAHGANDVANAVGPLAAIADVLTHSGAGVSEA
                     AKIPLWVMMVGAVGIALGLALFGPKLIRTVGSEITELDQMRAYCIAMAATLTVIVASQ
                     LGLPVSSTHIAVGGVFGVGFLREYLKSNYQRMVQDIKDHHPEGDQAAIDAFLVRFDAA
                     AIDDKGAMLRVLKAEAKARSEGALFSAHERKGLRKVYRGEVVKRSQLKKIIAAWLITV
                     PASATLGALFFFAIRGAMLP"
     gene            2265..3086
                     /locus_tag="VIS73_05560"
     CDS             2265..3086
                     /locus_tag="VIS73_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY5762652.1"
                     /translation="MPLSPRLIPFALALTLAGPADLFAQQGVDVGRPSSLRRLVPADS
                     LEQQSIQQYGQMLAAAREKNALAPDDHPQLIRLRQIAARITPFTGRFNQRASDWRWEV
                     NLIGSKQINAFCMPGGKIAFYTGILDTLELTDDEVAAIMGHEMAHALREHARERIAKS
                     QLTRLGTNILGSLLFEGRLAGAFDVGGSLLTLKFSRDDETEADLIGLELSARAGFDPR
                     AGISVWQKMGQATRGGPPQWLSTHPSGKSRIADMQRVVPQVMPIYEQSRKTAISR"
     gene            complement(3094..4218)
                     /locus_tag="VIS73_05565"
     CDS             complement(3094..4218)
                     /locus_tag="VIS73_05565"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5762653.1"
                     /translation="MTKVKGNARPLDGVRVLEFGSLIAGPFAARIMADFGAAVTKIEP
                     PEGDPLRSWRKLHEGTSLWWYVQSRNKHCRRLDLKTAEGRDAVLELVRESDIVVENFR
                     PGVMEKLGFGWDVLSAINPGLVMVRISGFGQDGPARDQPGFGAIGESMGGLRYVTGFA
                     DRPPVRTGVSIGDSIAALWAVSGALMALRHREVNGGKGQVVDVALYEAVFAMMESLVP
                     EYDVAGFVRERTGNIMPGITPSNTHTARDGKHIAIGANGDAIFGRLMRAIGRADLAED
                     PSLADNAGRDARRDEIYAVIDDWVAGRDAAEALRVLGAAAVPASPIYSVADMFADPQF
                     LAREMLLQRALPDGTPMKIPGVVPKLSATPGRVDWLGPKD"
     gene            4285..4902
                     /locus_tag="VIS73_05570"
     CDS             4285..4902
                     /locus_tag="VIS73_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEY5762654.1"
                     /translation="MTRTPNIATIVVLVAFVAATAFAQTPPLQPTPRDMRGPYYPDSL
                     PADRDADLTQIAGHAGTAQGQVLLLDGRVLDRQGKPMAGVQVEIWQTDANGRYIHSGD
                     RGKGKRDPGFQGFGATVTAADGRYAFRTVVPKSYGSRPPHVHARLLRDQREVLVTQIY
                     FPQATGEPGVSPDWARQRDLNQTLHINSAAGATDIRAAFDFVLAD"
     gene            4995..6101
                     /locus_tag="VIS73_05575"
     CDS             4995..6101
                     /locus_tag="VIS73_05575"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY5762655.1"
                     /translation="MSDWTRYIAETLGIESLNQPWLAMVLLTVIATVVIHFVAERLIR
                     RALRAAEQTENVWDDALLLSISRPLGVLIWVVGLALAVDFIRQHSGSDAQPILSLVPK
                     IREVGVILCLAWFLIRLIRRSAANYVRIHTANGDAVDRTTVDALAKLGRLSVLISAAL
                     IVMQTLGFSVSGVLAAGGIGGIAVGFAAKDLLANFFGGLTIYLDRPFSVGDWIRSPDK
                     QIEGTVEAISWRHTQIRAFNKNPIYVPNAVFTTIVVENPSRMSHRRIKETIGIRYEDL
                     AAMPAIVDDVRAMLVAHPEIASDQTLIVNFNEFADSSLNFFIYTFTKTKVWVDYHVVK
                     QDVLLKVAEIIASHGAEIAFPTRTVHLESDSAVA"
     gene            6274..6999
                     /locus_tag="VIS73_05580"
     CDS             6274..6999
                     /locus_tag="VIS73_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY5762656.1"
                     /translation="MHAALLTVATPAVPLRYLVASIAAHGLLAAAPMGTPDFTAASAP
                     QSLRVDLRRAAPPAPPAAELTAKRQAAPARPVRAKPLAVAPARLAAIAMPQPATVAPP
                     QPSLPAVDESGVAPPEPPAPLQLARAAPAAVDLAPLRSGPSDFHLLSLYTQQLGAAVN
                     QQRRYPPLARVRGWQGTTVLDLSIGPAGEVVSLSVASSSGFDLLDEQAIDMVREAQPL
                     PAPSVESGSLPLLVQLPVTFSLR"
     gene            6996..7973
                     /locus_tag="VIS73_05585"
     CDS             6996..7973
                     /locus_tag="VIS73_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5762657.1"
                     /translation="MTIHRRPARHLAAGVALWTVLASGSTVAGAADCPPAQRVADGVF
                     LLRGADAEPSPANGGKVANRVFLEGADGVVVIDPGPSPAAGRELACTVARHSSRPIRA
                     IVNTHPHPENVLANAAFSALPIHASAAAAHAMRGRCIDCRRQLLERIGTPASTVDDAT
                     SLAALIPGQLVDAPQTLTLAGRRLTLIPLGEAHSPGDLAILDHASGVLIGGDLVNVEA
                     LPDLHDGNTAVALAALGALRRMREIRQVIPGRGAPYDPAAMAVPADYLRALWDYALAR
                     VESPDGFVPPADVPPELLGFSADRHRQLLNLQHALREAEALWWQKEEKR"
     gene            8095..9063
                     /locus_tag="VIS73_05590"
     CDS             8095..9063
                     /locus_tag="VIS73_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5762658.1"
                     /translation="MFKRLAALAALAFTLGTACAATPEIRIGVLQYGTVNWELEVMQR
                     HGLAERAGVKLVIVPLALKDASSVAVLGGSVDMIVSDWLWVARQRIDGRDFVFAPYSR
                     TVGNIMVRPDAGITRFADLKGKRLGVAGGPLDKSWLFLRAYARKTLGDDAAKLVEPNF
                     AAPPLLNQLLLKGELPAVLNFWHFSARLQAAGMSRLLDMGEILDTLGIDEELPLLGWV
                     FRESWARDNATALAAFLRASTDAKKLMAASDGIWEELRPLTRAENDATLLALRDGYRA
                     GIPRRHDGAEEAARQAFAVLVQEGGEALMGPGKELPAGVFWKAAAQ"
     gene            9117..9899
                     /locus_tag="VIS73_05595"
     CDS             9117..9899
                     /locus_tag="VIS73_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5762659.1"
                     /translation="MNSSLPNWRPDRETTLRLLSLVAFVALWQLTAWIVEDATLPPPA
                     TVFARILSETLTGVLPQHLGVTLLRVGIAFFAALAIGAAIGMAMGRWQTLDRFLDGWL
                     VLGLNIPALVTIILCYVWFGLNDVAAIVAVAINKIPTVIVTVREGARAIDPRLMQVAD
                     AYRLSRWRRFTQVYLPQLHPYLFAAARTGLSLIWKIVLVVELLGRSEGIGFQLNVFFQ
                     LFDIAGILAYTLVFAGVVLIVEAFLLRPLERRLTRWRGNSHA"
     gene            9892..10671
                     /locus_tag="VIS73_05600"
     CDS             9892..10671
                     /locus_tag="VIS73_05600"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5762660.1"
                     /translation="MLEVRIDEKAYADRGGRRHVALRGLSLNVTPGEFVCLVGPSGSG
                     KSTLLNLIGGLDTQMNGSVRIAGEPPGAGVGFMFQEPRLMPWLTVLDNVRLVLDERLE
                     APAADARARELLAAMELDDVLDAYPSTLSGGMQRRVALARAFAIEPQLLLMDEPFVSL
                     DAPTAQRLRAMLVDLWQRRRTTVLFVTHDLREALSLADRVCFLSPAPGALVHEQRVAL
                     PRPRQPQDAAIGRLYDELMQAHPALLAGLAAPLHTLSEASA"
     gene            10668..11462
                     /locus_tag="VIS73_05605"
     CDS             10668..11462
                     /locus_tag="VIS73_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5762661.1"
                     /translation="MNVVAQAQQHGAAAPLLEIAQVDKSYGARKALAGVSVAIGAGEF
                     VALLGPNGAGKTTLFQLLSGLFVADAGSIRIGGVDIRRTPARALAQLGIVFQQPTLDL
                     DLSVEKNLVFNARLHGLGGSATRERISAELAAQGLAERRRDRVRELSGGNRRKVELAR
                     ALVHSPRVLLMDEATVGLDPGSRQQLLAQVRDLCRERGVAVLWATHLVDEAEDADRIV
                     LLHRGRVLADCPPAALVAKAGVSELGLAFEKLTAGATAGSDASIAE"
     gene            11491..12474
                     /locus_tag="VIS73_05610"
     CDS             11491..12474
                     /locus_tag="VIS73_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated
                     beta-propeller protein"
                     /protein_id="HEY5762662.1"
                     /translation="MIIRHRFLTAALLLSALAVPAAQARDTGHIFVSSEKDNAVTVLD
                     GQSLAVVKVIATGKRPRDMRLSLDRKQLYVIASDSQRLDIIDIAQLTVVGKVAVGEDP
                     EMFDLAADGKRIYVSNEEDAAVSVIDLASKKRVAEIEVGEEPEGVKLSPDGKRVYVTS
                     EVANMVHVIDTGSHQILANVVVGNRPRRFAITPDGGEVWVTNELGGAVTLIDGQTHAV
                     KASIPFKPKGFRDEDVTPVGITMTRDGKTAYVTLGRANHVAVVDVASRKVEDYILVGQ
                     RAWGATLDRKEARLFVVNGLSDDISVVDTASRKVLRSVPVGRVPHTVVIDD"
     gene            12485..13699
                     /locus_tag="VIS73_05615"
     CDS             12485..13699
                     /locus_tag="VIS73_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5762663.1"
                     /translation="MRAALPRFAGFAAVLLLAALLAHAGSARAAAKNKAVTLPIGYLQ
                     IVDDPRYEEDRLYARNLAQPTGRPDAGLETLIKESRFATSALGVTLTIERAEATDSAG
                     LVEEIVRLHGRGVRFFVIDAPGTVVGEVAAATRGKELLLFNVSAADDALRLGQCQAHL
                     LHTIPSDAMRGDALAQYLVSRKWRKVLMLQGPLADDALLAAAFARAAKRFGIKLVETR
                     NFVLSNDPRQREQGNVALLTANADYDAIFVADSDGEFARGLPYRSVLPRPVVGSEGLV
                     AAAWHWAWERHGAPQLSARFEKQAGRRMGESDWAAWIAGKAIVEAMQRTGGTDFKTLA
                     SYLRSAEITIDGFKGGKLSFRDWNRQLRQPLLIATHNAVIERAPIEGFMHQHNKLDTL
                     GYDRPEGGCKFQ"
     gene            13716..14525
                     /locus_tag="VIS73_05620"
     CDS             13716..14525
                     /locus_tag="VIS73_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5762664.1"
                     /translation="MTPIHALRVLGAVAGREVEKFVHQGGRFFSALVRPLLWLVVFAA
                     GFQNVFGVSIIPPYETYIPYQVYIAPGLLGMVLLFNGMQSSLAMVYDREMGMMRLLLT
                     APVPRPWLLFAKLVAGTLLSVLQAYAFLAVAFLVGVELPWAGLWTVLPALVLAGLMLG
                     SLGLLLSVNIKQLENFAGTMNFVIFPMFFISPALYPLWKLEESGAAWLHTAAQFNPFS
                     HAVELIRFALYGKFEPVACAVVAATLAVCFALAVVGYDPQRGLLKKSGRPG"
     gene            14692..15075
                     /locus_tag="VIS73_05625"
     CDS             14692..15075
                     /locus_tag="VIS73_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5762665.1"
                     /translation="MSFRLPAAGIAALLAFTTATALANDAAHEEKMKKLAANAGCFTC
                     HTIEHKGPKNGMNPIGPAWADVAAQYRDKPGAAEFLTRVVMEGSNPYASHWKNKVSGL
                     AMPPNAVAISEADTRQLVFWILSLK"
BASE COUNT         2578 a         5384 c         4701 g         2521 t
ORIGIN      
        1 acgaaaccct gcaccacccg gtcgccgaaa cggccaaggg tgtgctcgac gttatcaatc
       61 gcgccggagc cgaagccttc aagatcgcgg ttggcgccat ccccatgctg gtgatttcgc
      121 tggtgctcgt cgccctgctc aaggcagtcg gggcgatcga cctgctgacg gtgctgctgc
      181 ggcccctgct tgtgctgctc ggcatcgatc cggcgctgat cctgctcacc ctgaccaagg
      241 taatcgccgg tggcacggcg atgatgggcg tgatggatga catgctgcgc agcggcgaag
      301 ccagcgccag cctcatgaat ggctctgcgg gattcctgat tcacccgctc gatgttgccg
      361 gcgttgccgt gctgatctcg gccggtcgac gcgtcgcgca ggtgtggaaa ccggccgtcg
      421 ccggcggcat ggtcggcatt gccgtacgca cgctggggca tgcgctgggc agctaagcgg
      481 cggagtaccg cagccacccg ccgcaatcag gggagcatcg cgccgcggat tgcgaagaag
      541 aacagcgcgc cgagggtcgc cgaggcgggt accgtgatca gccaggctgc gatgatcttc
      601 ttcagttgcg agcgcttgac cacttcaccg cgatagacct tgcgcagtcc cttgcgctcg
      661 tgcgccgaaa acagcgcgcc ttcgctccgc gccttggcct cggccttaag tacgcgcagc
      721 attgcgccct tgtcgtcgat cgcagcggcg tcgaagcgga ccaggaaggc atcgatggcc
      781 gcctgatctc cctcgggatg gtgatccttg atgtcctgca ccatgcgctg gtagttgctc
      841 ttcaggtact cgcgcagaaa gccgacgccg aacacgccgc cgacggcgat atgcgtcgag
      901 ctgaccggca ggccaagctg gctggcgacg atgaccgtca gcgtcgccgc catcgcgatg
      961 cagtaggcgc gcatctggtc gagttcggtg atctcggagc cgaccgtacg gatgagcttg
     1021 ggtccgaaca gcgccagccc gagcgcaatg ccgaccgcac cgaccatcat cacccacagc
     1081 gggatcttcg ctgcctcgga aacgcccgcc ccggaatgcg tcagcacatc ggcgatcgcc
     1141 gccagcggtc cgacggcgtt ggcgacgtcg ttggcgccgt gggcaaagct gagtagtgcc
     1201 gcagcaaaga tcaatggcac ggtaaacatg cgattgacct tggccttgtc gccgaccgcc
     1261 tcgcctgtct gccgcgagta ccaggctacc cgcggtcgca cgaacagata cacgagaccg
     1321 ccgccaatca cgccccagaa gatcgccgag tagaagctga ccttgaccac ggcacgaacg
     1381 cccttgagca tcaggtaggt aatgaagaca ccggccatca gcgccaccag caccggcacc
     1441 acgcgccgcg ccgcgtccat catgtcgctc tggtaggtga ttcgccgctt gatcaggtaa
     1501 aggaaagccg cagcgatgag gccgccgagg accggcgaaa tgacccagct ggctgcgatc
     1561 gcgctgacct tgccccagct gacgacaccc caaccgccgg cggcgatgcc ggcaccgagg
     1621 acgccaccaa caatcgagtg cgtggtcgaa accggtgcgc cgatggcagt ggcgatgttg
     1681 agccagatcg ctccggcgag cagcgcaccc atcatcaacc agacgaaggt atcggcgctg
     1741 ccgatctggt cctggctgat gatgccgccg cgaatcgtcg ccaccacatc accgccggca
     1801 atcagggctc ccgcggcctc gcagacaccg gcgatgacca gcgcaccgat catcgtcaat
     1861 gcccgcgaac cgaccgccgg gccgacgttg ttggcgacgt cgttggcgcc gatgttcatc
     1921 gccatgtagc cgccgatcat tgccgcgacc accagcagga agctgccatc gccgcgcgcc
     1981 caagtgaaga acatgacgcc gacgatgaac aggaaggcga gtccgaagcg caacagctcg
     2041 ggctgcgact tctgcgccgc cttgtcgaat tctttgatgt ccttgagttc catcgggaat
     2101 aattgcggca ataccgcggt ctgatccggg cgatcggggc gcgcattgta agccgacagt
     2161 cacgcgacgg tcatcttcga gtcgcgttcc ggcaaatatg cgattgcaat tctttcattt
     2221 tttcaatgta ttagcaccat aagaccagag tacacagcac cgccatgcca ctgagcccca
     2281 ggctgatccc gtttgcgctg gcgctgacac tcgccggccc tgccgactta tttgcccagc
     2341 agggggtcga tgtcggccgg ccgtcgtcgc tgcgccggct ggtcccggcg gattcgctcg
     2401 agcagcagtc gatccagcag tacgggcaga tgctcgccgc cgcgcgggaa aagaacgcgc
     2461 tggcgcccga cgatcacccg cagctcattc gcctgcgcca gattgccgcg cgcatcacgc
     2521 ctttcactgg ccgcttcaac cagcgcgcca gcgactggcg ctgggaagtc aatctgatcg
     2581 gctccaagca gatcaacgcc ttctgcatgc caggcggcaa gatcgccttc tatacgggga
     2641 ttctcgacac gctggagctc accgacgacg aggtcgcggc gatcatgggc cacgagatgg
     2701 cgcacgcgct gcgcgaacac gcgcgcgagc ggattgccaa gtcccagctg actcgcctgg
     2761 gcaccaacat cctcggcagc ctgttgttcg agggccgcct ggccggcgcc ttcgacgtcg
     2821 gcggcagcct gttgacgctg aagttctcgc gcgacgacga aaccgaagcc gacctgatcg
     2881 gcctcgaact ctccgcccgc gccggcttcg acccgcgcgc cggcatttcg gtctggcaga
     2941 agatggggca ggcgacccgc ggcgggccgc cgcaatggct atcgacgcac ccctcgggca
     3001 agtcccggat cgccgacatg cagcgcgtcg tgccgcaggt catgccgatc tacgaacaga
     3061 gtcgcaagac cgccatttcc cgctgaagcc ggttcaatcc ttcgggccga gccagtcgac
     3121 gcggcccggc gtcgccgaga gcttgggtac gacgccggga atcttcatcg gcgtaccgtc
     3181 gggcaaggcc cgctgcagca acatctcgcg ggcgagaaac tgcggatcgg caaacatgtc
     3241 ggcaaccgaa tagatcgggc tcgccggcac cgcagccgcg ccgagcacgc gcaaggcctc
     3301 ggcagcgtcg cgcccggcca cccagtcgtc gatcaccgca tagatttcgt cccgccgcgc
     3361 atctcgtccg gcattgtcgg cgagggacgg gtcctcggcc agatcggccc ggccgatcgc
     3421 gcgcatcagg cggccgaaga ttgcgtcgcc gttggcgccg atggcgatgt gcttgccgtc
     3481 gcgagccgtg tgcgtattcg acggcgtgat ccccggcatg atgttgccgg tccgctcgcg
     3541 aacgaaaccg gccacgtcgt attccggcac caggctctcc atcatcgcga acacggcctc
     3601 gtagagcgca acgtcgacca cctgcccctt cccgccattg acctcgcgat ggcgcaacgc
     3661 catcaatgcg ccggacaccg cccacagcgc cgcgatcgag tcgccgatcg acaccccggt
     3721 ccgcaccggt ggccggtcgg caaagccggt tacgtagcgc aggccaccca tcgactcgcc
     3781 gatcgcgccg aatccgggct gatcgcgagc cgggccgtcc tgcccgaagc cggaaatgcg
     3841 caccatgacc agccccggat tgatcgccga cagcacatcc cagccgaatc ccagtttctc
     3901 catgacgccg ggccggaagt tctctaccac gatgtcggac tcgcgcacga gttcgagcac
     3961 cgcatcgcgg ccctcggccg tcttgagatc gagccgccgg cagtgcttgt tgcgcgactg
     4021 cacgtaccac cagagtgaag ttccctcatg cagcttgcgc catgagcgca gggggtctcc
     4081 ctcgggtggc tcgatcttgg tgactgcggc accgaaatcg gccatgatcc gcgccgcgaa
     4141 cggaccggct atcaggctgc cgaactcgag cacgcgcacg ccatctagcg gccgcgcatt
     4201 acccttgact ttagtcatac tagactcccg gaagtcgtgt ccaaatcatc cctcaattgc
     4261 ctacgcgcca cccggagacc cagcatgacg cgcaccccga acatcgcgac aatcgtcgtt
     4321 ttggtggcct tcgtggctgc caccgcattt gcgcaaaccc cgcctctgca gccaacgccg
     4381 cgcgacatgc gcggccccta ctaccccgac agcctgccgg ccgaccgcga cgccgacctg
     4441 acacagattg ccggccacgc cggaaccgcg caaggccaag tcctcttgct cgacggccgc
     4501 gttctggatc gacaaggcaa gccgatggcc ggcgtacagg tggaaatctg gcagaccgac
     4561 gccaacggtc gctacatcca cagcggtgat cgcggcaaag gcaaacgtga tcccggcttt
     4621 cagggattcg gcgccaccgt gaccgctgcc gacggccgct acgcctttcg cacggtcgtg
     4681 cccaagtcct acggcagccg gccaccacac gtgcacgcac gcctgctgcg cgaccagcgc
     4741 gaagtgctgg tcacgcagat ctatttcccg caggcgaccg gcgaaccggg cgtctctccc
     4801 gattgggcgc gacagcgcga tctcaaccag acactgcaca tcaactctgc tgcgggcgcg
     4861 acagatatcc gcgccgcctt cgacttcgtg ctcgccgact gagcaacgct gaagcggtct
     4921 ggtgactgtg tcagactgaa aagggaatgc gcgccggttg aggcgcgcgc cgctttcagc
     4981 agggaggaaa tcgcatgtcc gactggaccc gctacatcgc ggaaacgctt ggaatcgaat
     5041 cgctcaacca gccctggctc gccatggtgc tgctcaccgt catcgccacc gtcgtcatcc
     5101 acttcgtcgc cgaacgcctg attcgccgcg cgttgcgcgc tgcggaacaa acagaaaacg
     5161 tctgggacga cgcactgctg ctgtcgatct cgcgcccgct cggcgtgctg atctgggtcg
     5221 tcggcctggc cctggcggtc gacttcatcc gccagcactc gggcagcgac gcacagccga
     5281 tcctgtcgct ggtaccgaag atccgtgagg ttggcgtgat cctctgtctc gcctggttcc
     5341 tgatccggct gatccgccgg agcgccgcca actacgtgcg gatccacact gcgaacggcg
     5401 acgctgtcga ccgcacgacc gtcgatgcgc tggccaagct tggccgcctc agcgtgctca
     5461 tctcggcggc gctgatcgtc atgcagacgc tgggattcag cgtttccggc gtcctcgccg
     5521 ccggcggcat cggcggcatc gcggtcggtt tcgccgccaa ggacctgctc gccaacttct
     5581 tcggcggact gaccatctac ctcgaccgcc cgttcagtgt tggcgactgg atccgctcgc
     5641 ccgacaagca gatcgagggc acggtcgagg caatcagctg gcggcacacg cagatccgcg
     5701 ccttcaacaa gaatccgatc tacgttccca acgccgtgtt cacgacgatc gtcgtcgaga
     5761 atccgtcgcg catgtcacat cggcggatca aggaaaccat cggcatccgt tacgaagacc
     5821 tcgcagcgat gcctgccatc gtcgatgacg tccgcgcgat gctcgtcgcg caccccgaaa
     5881 tcgcttccga ccagacgctg atcgtcaatt tcaacgagtt cgccgattcc tcgctgaatt
     5941 tcttcatcta taccttcacc aagaccaagg tctgggtcga ttaccacgtg gtcaagcagg
     6001 atgttctgct taaggttgcc gagatcatcg ccagccacgg cgccgagatc gccttcccga
     6061 cccgcaccgt gcatctcgaa tcggattcgg ccgtggcctg aacctgcccc gggggcagac
     6121 accgaacgag gtcgctccct cgcggctgtg ccaattcgtt acaggtgtca cgcaccccgg
     6181 ccggacgctt gtagcccgat catgagccgg taatggcccg cggggcccac cgacggcgag
     6241 aacttgtgga atggaaaatg catcgaagca ggtatgcacg ccgcgctcct caccgtcgcc
     6301 acgcctgcgg ttccgctccg ctatctggtc gcgtcgatcg ccgcccacgg cctgctcgcg
     6361 gcggcgccga tggggacgcc cgatttcacg gcagccagcg ccccgcaaag ccttcgcgtc
     6421 gacctgcggc gtgccgcgcc accggcgccg ccggccgccg aattgacggc caaacgccag
     6481 gccgcgccgg cccgaccggt tcgcgccaaa ccactcgcgg tcgcgccagc acgcctcgct
     6541 gcaatcgcca tgccgcaacc ggcgaccgtc gcgccgcctc agccctcgct ccccgcagtg
     6601 gacgagagcg gcgtcgcccc gcccgagccc ccggcgccgc tacaactggc gcgggcggca
     6661 ccggccgccg tcgacctggc gccgctgcgc tccggtccga gcgactttca cctgctgtcc
     6721 ctctataccc agcagctggg agcggccgtc aaccagcaac gccgctatcc gccgctcgcc
     6781 cgcgtgcgcg gctggcaagg caccacagtc cttgacctca gcatcgggcc ggccggcgaa
     6841 gtggtctcgc tgagcgttgc cagctcgtcg ggtttcgacc ttctcgacga acaggcgata
     6901 gacatggtcc gcgaggccca gcccctgccc gcgccgtcgg tggaatcggg ttcgctgcca
     6961 ttgctggttc agcttccggt caccttctcg ctgcggtgac gattcaccgc cggccagcgc
     7021 gccaccttgc cgccggagtc gcgctctgga ccgtgttggc gtctggctcg accgtcgctg
     7081 gcgcggccga ctgcccgcca gcgcaacgcg tagccgacgg cgtcttcctg ctgcgcggcg
     7141 ccgatgccga accgtccccg gcgaacggtg gcaaggtcgc caatcgagtc tttctcgaag
     7201 gcgctgacgg cgtcgtcgtc atcgaccccg gaccgtcgcc ggccgccggc cgcgaactcg
     7261 cctgtaccgt cgcccgccac agcagccgac caatccgcgc catcgtcaat acccatccgc
     7321 accccgagaa cgtgcttgcc aatgcggcgt tttcggcgct gcccatccac gccagcgccg
     7381 ccgcagcgca cgcgatgcgc ggacgctgca tcgactgccg gcggcaactg ctcgaacgga
     7441 tcggcacgcc ggcaagcacc gtagacgacg ccaccagcct tgcggcgctg attcccggac
     7501 agcttgtcga tgcgccgcag acccttacgc tagcagggcg ccgcctgaca ttgataccgc
     7561 tcggcgaagc gcacagcccc ggcgacctgg cgattctcga ccacgccagc ggcgtgctga
     7621 tcggcggcga tctggtcaat gtcgaagcgc ttcccgatct gcacgatggc aacaccgcag
     7681 tggcactcgc tgcgctcggc gcgctgcgca ggatgcgcga gatccggcaa gtaatcccag
     7741 ggcgcggcgc gccctacgat cccgccgcga tggcggtccc ggccgactac ctacgcgcgc
     7801 tctgggatta cgcgctggcc cgcgtcgaaa gccccgatgg attcgttccg ccggccgatg
     7861 tgccacccga actgctcggc ttctcggccg atcgccaccg acagctgctc aatctgcagc
     7921 acgcgctgcg cgaagccgaa gcgctctggt ggcaaaaaga ggagaaacgc tgacccgcgg
     7981 actggcgtgg cacggacatt gcggacgaca atgcaccagg acccggcaca gaccggtgcg
     8041 cggaaacgac ggccgaccgg cggccggccg cttcgcgcca acgaggagac accgatgttc
     8101 aaacgattgg ccgcgcttgc ggcactggca ttcacgctgg gcaccgcctg cgccgcaacg
     8161 cccgagatcc gcattggcgt gctgcagtac ggcaccgtca actgggaact cgaggtcatg
     8221 cagcgccacg gcctcgccga gcgcgccggc gtcaagctgg tcatcgtccc actcgcgctg
     8281 aaggacgcgt cgagcgtcgc cgtgctcggc ggctcggtcg acatgatcgt cagcgactgg
     8341 ctttgggtgg cccgccagcg catcgacgga cgtgatttcg tctttgcgcc ctactcgcgc
     8401 acggttggca acatcatggt tcgccccgat gccggcatca cgcgtttcgc cgatctcaag
     8461 ggcaagcgct tgggcgttgc cggcggcccg ctcgacaaga gctggctctt cctgcgcgcc
     8521 tacgcgcgca agacgctggg cgacgacgcc gccaaactgg tcgaaccgaa tttcgccgca
     8581 cccccactct tgaaccagct gctgctcaag ggcgagttgc cggccgtgct caacttctgg
     8641 cacttcagtg cacggctgca ggcagccggg atgagccgcc tgctcgacat gggcgagatt
     8701 ctcgacacgc tcggcatcga cgaagaactg ccgctgctcg gttgggtctt ccgtgagtcg
     8761 tgggccaggg acaacgcgac ggcactggcc gcctttctgc gcgcctcgac tgacgccaag
     8821 aaactgatgg ccgcgtctga cgggatctgg gaagagctgc gcccgctcac gcgcgccgaa
     8881 aacgacgcga cgctgctcgc gctgcgcgac ggttaccgcg ccggcatccc gcgccgtcac
     8941 gacggcgccg aggaggcagc gcggcaggct ttcgccgtgc tcgtgcaaga aggcggcgaa
     9001 gcgctgatgg gccccggcaa ggagctgccg gctggcgtct tctggaaagc cgcggcgcag
     9061 tgagtgaggc aagccatgcc aaactggccg caacggcgac tggcgctgcc cgtcgcatga
     9121 actcgtcgct gcctaactgg cgccccgatc gcgaaacgac gctgcgcctg ctctcgctgg
     9181 tcgccttcgt cgcgctctgg cagctcaccg cctggatcgt ggaggatgcg acgctgccgc
     9241 caccggcgac ggtctttgcc cgcatcctca gcgagacgct gaccggcgtg ctgccgcagc
     9301 atctgggcgt gaccctgctg cgcgtcggca ttgccttctt cgccgcgctc gccatcggtg
     9361 ctgcgatcgg catggcgatg gggcgctggc agacgctcga ccgctttctc gacggttggc
     9421 tggttctcgg cctcaacatc ccggcgctgg tgacgatcat cctctgctac gtctggttcg
     9481 gcctcaacga cgtcgccgca atcgttgccg tcgccatcaa caagattccg acggtcatcg
     9541 tcaccgtacg ggaaggcgcg cgcgcgatcg atccgcgcct gatgcaggtg gccgacgcct
     9601 accgactgtc gcgctggcgc cgcttcacgc aggtctacct accgcagctg cacccctacc
     9661 tgttcgccgc cgcacggact ggcctgtcgc tgatctggaa gatcgtgctg gtcgtcgagc
     9721 tgctgggccg cagcgaaggg atcggctttc agctcaacgt tttcttccag ctcttcgaca
     9781 tcgctggcat cctcgcctac acgctggtgt tcgccggcgt agtgctgatc gtcgaagcct
     9841 tcctcctgcg cccgctcgaa cggcgcctga cccgctggcg gggtaattcc catgcttgag
     9901 gtccgcatcg acgaaaaggc ctacgccgac cgcggtggcc ggcggcacgt ggcgctgcgc
     9961 gggctgtcgc tcaatgtcac gcccggcgaa ttcgtctgcc tggtcggccc ctcgggcagc
    10021 ggcaagagca cgctgcttaa tctgatcggt ggcctcgata cgcagatgaa cgggtcggta
    10081 cgcattgccg gcgaaccgcc aggcgccggt gtcggcttca tgtttcagga gccgaggttg
    10141 atgccctggc tcaccgtgct cgacaacgta cgcctggtgc tcgatgagcg cctcgaagcg
    10201 ccggcagccg atgcgcgcgc gcgcgaattg ctggcggcga tggaactgga cgacgtcctc
    10261 gatgcctatc ccagcaccct ctcgggcggc atgcagcgac gtgtcgcgct ggcccgggct
    10321 ttcgccatcg aaccgcagct actgctcatg gatgaaccct tcgtgtcgct tgacgcgcca
    10381 actgcgcaac gcctgcgcgc catgctcgtc gatctctggc agcggcgccg caccaccgtc
    10441 ctattcgtca cccacgacct gcgcgaagcg ctttcgctcg ccgaccgcgt ctgcttcctc
    10501 tcgcccgcac ccggagccct cgtgcacgag caacgggtcg cgctgccacg cccgcgccag
    10561 ccgcaggatg cggcgatcgg ccgcctttat gacgaactga tgcaggcgca tccggccctg
    10621 ctcgccggcc tcgctgcgcc gctccacacc ctttcagagg caagcgcatg aacgtcgtgg
    10681 cgcaggcaca acaacacggc gctgccgccc cgctgctcga gatcgcgcag gtggacaaat
    10741 cctatggcgc gcgcaaggca ctggccggcg tcagcgtcgc catcggagcc ggcgagttcg
    10801 tcgccctgct cggccccaac ggcgcaggca aaaccacgct gttccagctg ctctccggcc
    10861 tctttgtcgc cgacgccggc agcatccgca tcggcggcgt ggacattcgc cgtaccccgg
    10921 cgcgtgcact ggcgcagctt ggcatcgtct tccagcagcc gacgctcgac ctcgaccttt
    10981 cggtggaaaa gaacctcgtc tttaatgccc gcctgcacgg cctcggcggc agcgcgacgc
    11041 gcgaacgcat cagcgccgag ctcgccgcgc agggcctcgc cgaacgccgg cgcgatcgcg
    11101 tccgcgagct ttccggtggc aaccggcgca aggtcgaact cgcacgcgcg ctcgtgcatt
    11161 cgccgcgcgt gctgctgatg gacgaagcaa ctgtcggcct agaccccggc tcgcgccagc
    11221 agttgcttgc gcaggtacga gatctctgcc gcgaacgcgg cgtcgccgtg ctgtgggcca
    11281 cccacctcgt cgatgaagcc gaagacgccg accgcatcgt cctgctgcac cgtggccggg
    11341 tactcgccga ctgcccaccc gccgcgctgg tcgccaaggc tggcgtcagc gagcttggcc
    11401 tcgccttcga aaaactcacc gccggcgcca ctgccggctc cgacgcgtcc atcgccgaat
    11461 aatcacaact cgataaacgg accccaaccc atgatcatca ggcatcgatt cctcaccgcc
    11521 gcgctgctgc tctcagccct tgccgtgccc gccgctcagg cacgcgacac cggccacatc
    11581 ttcgtcagca gcgagaagga caatgccgtg accgtgctcg acggccaatc gctcgccgtc
    11641 gtcaaggtca tcgccaccgg caagcgcccc cgcgacatgc gcctctcgct cgatcgcaaa
    11701 caactctacg tgatcgccag cgattcgcag cgcctcgaca tcatcgacat cgcgcagctc
    11761 acggtggtcg gcaaggttgc ggtcggcgaa gacccggaaa tgtttgacct cgctgccgac
    11821 ggcaagcgga tctacgtttc caacgaggaa gacgcggcgg tgtcggtgat cgacctggcg
    11881 agcaagaagc gcgtggccga aatcgaggtt ggcgaggagc ccgaaggcgt caagctctcc
    11941 cccgacggca agcgtgtcta tgtgacctcc gaagtggcga acatggtcca cgtcatcgac
    12001 accggcagtc accagattct cgccaacgtc gtcgtcggca accggccgcg gcgtttcgcg
    12061 atcactcccg acggcggcga agtctgggtg accaacgaac tcggcggcgc ggtgacgctg
    12121 atcgacggcc agacccacgc cgtgaaggcg agcattccgt tcaagcccaa gggcttccgc
    12181 gacgaggacg taacgccggt aggcatcacc atgacccgcg acggcaagac cgcctacgta
    12241 acgctgggcc gcgccaacca cgtggccgtg gtcgacgtcg ccagccgcaa ggtcgaggac
    12301 tacatcctcg tcggccagcg cgcctggggc gctaccctcg accgcaagga agcgcgcctg
    12361 ttcgtggtta atggcctctc cgacgacatc tcggtcgtcg ataccgcctc gcgcaaggta
    12421 ctgcgctcgg tgccggtggg gcgcgtgccg cacaccgttg tcatcgacga ttaaggccct
    12481 cttgatgcga gctgccctgc cccgctttgc cggcttcgcc gccgtactgc tcctcgccgc
    12541 gctgctggct cacgccggca gcgctcgcgc ggctgcgaag aacaaggccg tcacgctgcc
    12601 gatcggctac ctgcagattg tcgacgaccc gcgctacgag gaagaccggc tctacgcacg
    12661 caacctcgca cagcccaccg gccgccccga cgccggactg gaaacgctga tcaaggaatc
    12721 gcgcttcgcc acctcggcac tcggcgtcac gctcaccatt gagcgcgccg aagcgactga
    12781 cagcgccggg ctggtcgagg aaattgtgcg cctgcacggg cgtggtgtgc gcttcttcgt
    12841 catcgacgcg ccgggcacgg tggtcggcga agtcgccgcc gccacgcgcg gcaaggagct
    12901 gctgctcttt aacgtcagcg ctgcagacga tgcgctgcga ttggggcaat gccaggccca
    12961 tctgctacac acgattccca gcgacgcgat gcgcggcgac gcgctggcgc agtatctggt
    13021 ctcgcgcaag tggcgcaagg tgctgatgct gcagggccct ctggccgacg acgcgctgct
    13081 cgccgccgcc ttcgcgcgcg ccgccaagcg cttcggcatc aagctagtcg agacgcgaaa
    13141 cttcgtgctc tccaacgacc cgcggcagcg cgagcagggc aacgtcgcgc tgctcacagc
    13201 caacgccgac tacgacgcga tcttcgttgc tgacagcgac ggcgagttcg cacgcggcct
    13261 gccctaccgc agcgtgctgc caaggccggt ggttggaagc gaaggcctcg tcgccgccgc
    13321 ctggcattgg gcctgggagc gccacggtgc gccgcaactg tcggcgcgct tcgagaaaca
    13381 ggccgggcga cgcatgggcg agtccgactg ggccgcctgg atcgcgggca aggccattgt
    13441 cgaggcgatg caacgtaccg gcggcaccga tttcaagacg ctggcctcct acctgcgtag
    13501 cgccgagatc accatcgacg gcttcaaggg cggcaagctc tcgtttcgcg actggaacag
    13561 gcagctgcgt cagccgctgc tgatcgccac ccacaacgcc gtcatcgaac gcgcgccgat
    13621 agagggtttc atgcatcagc acaacaagct cgacacgctg ggttacgacc gccccgaggg
    13681 cggctgcaag ttccaatgag cgcgggccag cgaccatgac cccgatccac gccctgcgag
    13741 tcctcggcgc cgtcgccggc cgcgaagtcg agaagttcgt ccatcagggc ggccgcttct
    13801 tctcggcgct ggtgcgcccg ctgctgtggc tggtggtctt tgccgccggg ttccagaacg
    13861 tctttggcgt ctcgatcatt ccgccctacg aaacctacat tccctatcag gtttacatcg
    13921 cgccggggct gctcggcatg gtcctgctct ttaacggcat gcagtcttca ctggcgatgg
    13981 tctacgaccg cgaaatgggc atgatgcggc tcctgctcac cgcaccggtg ccgcgaccct
    14041 ggctgctctt cgccaagctc gtcgccggca cgttgctctc ggtgctgcag gcctacgcct
    14101 tcctcgccgt cgcctttcta gtcggtgtcg agctgccctg ggccggattg tggaccgtgc
    14161 tgccggcgct agtgctcgcc ggcctcatgc tcggctcgct cggcctcctg ctctcggtca
    14221 acatcaagca attggagaac ttcgccggca caatgaactt cgtcatcttc ccgatgttct
    14281 tcatctcccc ggcgctctac ccgctgtgga agcttgagga atcgggcgcc gcctggctgc
    14341 atacggccgc gcagttcaac ccctttagcc acgccgtcga actgatccgc ttcgcgctct
    14401 acggcaagtt cgaaccggtc gcctgcgccg tcgtcgcggc cacgctggcg gtgtgttttg
    14461 cactggccgt ggttggttac gacccgcagc gggggttatt gaaaaagagt gggcggccgg
    14521 ggtaggcacc agctgcctag cgctagacgc ctgacgcctg actgatgacc gaagaggaca
    14581 tgccatcgcg ccccagaact tgccgtggcg cgagacttgc atgcgaattt cactgagatg
    14641 cgcgccgagg tgcgtgcgat ttttcgacca cccgaatctg gagaacgtcc catgagcttc
    14701 cgactacctg ccgccggcat cgccgccctg cttgccttca cgaccgcgac tgcgctggcc
    14761 aacgatgccg cccacgaaga gaagatgaag aagctggccg ccaacgccgg gtgctttacc
    14821 tgtcacacga tcgagcacaa gggaccgaag aacggcatga acccgattgg cccggcgtgg
    14881 gccgatgtgg cggcacagta tcgcgacaag cccggggccg ccgaattcct gacccgcgtg
    14941 gtgatggaag gcagcaaccc ctacgccagc cactggaaaa acaaggtatc aggcctagcg
    15001 atgccgccga acgcagtggc gatctcggag gccgatacgc gccagctggt gttctggatt
    15061 ctatcgctca agtagccgga tagcgcaccg cttcgatcat ctgccgccaa ggcagatgac
    15121 ggggcgcgcc gctactcgat cgttagtcgc acgaaccagc gcgcgccggg gtcgatgtcg
    15181 ctgc
//
LOCUS       DATDAA010000171        11149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_152514,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000171 DATDAA010000000
VERSION     DATDAA010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11149
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152514"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VIS73_05630"
     CDS             <1..193
                     /locus_tag="VIS73_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762666.1"
                     /translation="AIKHPAYPLLFDPQTAGGLLASVPAAQVDACLAALHELGYARAT
                     RIGRVLPAGDALEPISLRC"
     gene            210..815
                     /locus_tag="VIS73_05635"
     CDS             210..815
                     /locus_tag="VIS73_05635"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5762667.1"
                     /translation="MSRSIDFFETQFRRQLADSDIALNPFEAQALPFLHGDVLDFGCG
                     LGNLALAAAARGCRVLALDGAPTAIAHLQRRAGQAGLAINAVESDLRDYVIAQDFDAV
                     VAIGILMFFDCVTAQRQLAALRERVRPGGIAVVNVLTTDTTFRDMFDPASYCLFERDA
                     LRPAFAGWSIEYESADTFSAPGGTKKCFATLIARRTATGLP"
     gene            890..1753
                     /locus_tag="VIS73_05640"
     CDS             890..1753
                     /locus_tag="VIS73_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5762668.1"
                     /translation="MIPMPRLISRSALALALMLGAAVAQADVLRVSAIPDEAPTELQR
                     KFKPLGDYLEKETGMKVQFTPVSDYAAVVEGLAAKKIDLAWLGGFTFVQAKLRTNGEA
                     MPIIQRAEDEVFTSKFIVPLDSPIKSFADLKGKTFAFGSPSSTSGHLMPRHFLMKDGI
                     DPDRDFKAIAFSGAHDATVAFVASGKAEAGALNASVMDKLIAKGDENAKRVRVLATTP
                     PYYDYNWTVRPGLDPALREKIAQAFLKLDPANPAHKELMELQRASKFVATKASNYDGI
                     EGAAKSAGLIK"
     gene            1761..2609
                     /locus_tag="VIS73_05645"
     CDS             1761..2609
                     /locus_tag="VIS73_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEY5762669.1"
                     /translation="MSFELAGIGLTHGNGFRALGSVSLALAPGERVAIIGPSGAGKTT
                     LLQLLGTALRPSDGRLEVLGVRPWQLSGGELRRLRTRIGTVHQSPPIPPRLRVITAIL
                     AGRLGRWPLWRSLGSLLYPADIAGARDALARFDLADRLFDRCDRLSGGQLQRVGIARV
                     LYQQPDLILADEPVSALDPTLADAAIGELVAECERRGASLAASLHAVDLALKWFPRIV
                     GLRGGEVVFDRPTETVSAKMLRELYAAEGGVLPTQANDPDRLRLEPAALAAEAKVIKL
                     GTCRGR"
     gene            2611..3450
                     /locus_tag="VIS73_05650"
     CDS             2611..3450
                     /locus_tag="VIS73_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5762670.1"
                     /translation="MSTATALPLTDRDPDARARLGWALVALVLLWPLLVLAEFKPWAL
                     FDASNLRVMGSFLAGFLPPETSTEFLGYLLKATLETLAIATAGIALALLLAIPLAILC
                     TRALSVSLIGPGPGHRGGIALRALGRALLTFLRAIPEIVWALMFVRVLGLGPAAGVLA
                     LAITYGGMLGKVYTEILESTDTRPARTLLESGSGRLAALAYGLLPNAAQELTSYTVYR
                     WECAVRASVIMGFVGAGGLGQLMDQSMKMLNGGEAATILFVFLLLVLLADALSSVLRR
                     ALA"
     gene            3447..4289
                     /gene="phnE"
                     /locus_tag="VIS73_05655"
     CDS             3447..4289
                     /gene="phnE"
                     /locus_tag="VIS73_05655"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEY5762671.1"
                     /translation="MSTTRRTAGGARAFGESECRPRGAADFCLSCVLTSGLLLAAVVG
                     SFVYLGIDFGELFSGESAASMGRFAASFFPPDLDPAFLWKTIYGALQTFAVAAVGTLL
                     AAGAGLLLALPASGRHGIAAMRVSRFSLNLLRSVPELVWATLMVLAAGLGPFAGTLAL
                     ALHTTGVLGRLFAEAIENAPNEPEQSLLESGSGSGIAFVYGTLPLVWPQCIAYGLYRL
                     EMNIRMAAVLGFVGAGGLGQMLYFHLSIFQQAQASTVLIAMFVLVLAVDFLSDRLRAR
                     MSRT"
     gene            4856..5602
                     /gene="ccmC"
                     /locus_tag="VIS73_05660"
     CDS             4856..5602
                     /gene="ccmC"
                     /locus_tag="VIS73_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEY5762672.1"
                     /translation="MSNRLLNWFKFASPQSFYPLAGKMIPWFWALTAVLGISGLVLGF
                     GYAPTDAQQGEGYRIIFLHVPASWMAMFIYVVMACWAGLGLILNTRLSGMMASALAPT
                     GAMMAFLSLWTGSLWGKPMWGTWWVWDARLTSSLILLFLYIGFIALQSAITDARRADK
                     AGAVLALVGVINVPIIYFSVKWWNTLHQGYTVAPQKASIDPAMLWGMLLMSAAFWMYS
                     IAVTLTRARAIILERERHTEWVRGAVPAKA"
     gene            complement(5621..6448)
                     /locus_tag="VIS73_05665"
     CDS             complement(5621..6448)
                     /locus_tag="VIS73_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="HEY5762673.1"
                     /translation="METLPTDFLAVLALVFLWGARHGFDADHLATIDGLTRYNARQRP
                     AFARHCGVLFSLGHGAVVVVVAVAVSALAANWQVPAWLAHLGVWISIVFLIALAFLNL
                     RAVMTTPRHELVSPAGIRTRLLGRFQRSGSPLMIAGVGALFALSFDTLSQAALFALTA
                     QQFGGWGHGLALGLAFLLGMLICDGLNGLWIARLLRQADRRAAIASRVMGVAIAAMAL
                     LVAGFGLAKYLSPAIDAWAEDAGMLTGLVVVATILLSFFVALRLSLGVAARTARAAG"
     gene            6610..7275
                     /locus_tag="VIS73_05670"
     CDS             6610..7275
                     /locus_tag="VIS73_05670"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HEY5762674.1"
                     /translation="MHIELTPVQARVIGCLIEKEITTPEQYPLSLNALTNACNQKSNR
                     EPVLELDDRTVQDTVDGLIKVYLVSEQTGYGSRVAKYKHRFCNSDYGKLHFTPQELAV
                     VCELLLRGPQTPGELRSHASRMATLRDVHEVEALLEGLAAREDGPFVVRLARQPGKRE
                     ARYAHLFSGAVTGDVEPAAHATAAGDSGARLEERVAQLEAEVATLRERLAVLESPPES
                     QLP"
     gene            complement(7334..7738)
                     /locus_tag="VIS73_05675"
     CDS             complement(7334..7738)
                     /locus_tag="VIS73_05675"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEY5762675.1"
                     /translation="MNDWPLAARLAVAAGALAAALAVALGAASAHALKPLLLVNDPAG
                     WFETARRYHELHAIGLVAVGLAIALRPASRWITAAAVLLAAGIVLFSGSLYLRSLFGI
                     QELRSLTPLGGVALMAGWVALALGVILPRRPR"
     gene            complement(7735..8430)
                     /locus_tag="VIS73_05680"
     CDS             complement(7735..8430)
                     /locus_tag="VIS73_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5762676.1"
                     /translation="MSELLQAIEIETGATPDAAVIWLHGLGADGSDFVPIVPELRLPP
                     ALKLRFVFPHAQQMPVTCNGGYVMPAWYDIISLDPDNRVVDGEGIERSRQAIEALIAR
                     EVARGIATERIVIAGFSQGGAIAYATALRHPAPLAGVIALSTYIPEPDPLFAGASEAN
                     RTIPLFAAHGVEDDVVSLKLGTRARDELLRRGYVVDWHEYPMPHSVCIEEIVDIGRWL
                     TQTLAPTDAGAPR"
     gene            complement(8683..8973)
                     /locus_tag="VIS73_05685"
     CDS             complement(8683..8973)
                     /locus_tag="VIS73_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762677.1"
                     /translation="MVMMKPSASRPFTISARVRCGGGPRANQKATGPAGSVTVTGCGA
                     IWMCAMNRLRMPATLTRKLSALPLSMTSPRALPVALVATGTPASVKPSRRVS"
     gene            complement(9212..9634)
                     /locus_tag="VIS73_05690"
     CDS             complement(9212..9634)
                     /locus_tag="VIS73_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY5762678.1"
                     /translation="MGNLTRYDPWEDLFRGFFVKPVDLPGGMSAMTPQQIKLDVREDK
                     DAYRVHAEMPGVKKDDIHVTVEGSTVSISAEVKQENEVKEGERLLRSERYYGQVSRSF
                     QLGQDVDDAKADAKFADGVLELTLPKKATMGSKRLPIH"
     gene            complement(9786..10373)
                     /locus_tag="VIS73_05695"
     CDS             complement(9786..10373)
                     /locus_tag="VIS73_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5762679.1"
                     /translation="MIALRTCWAAIALWAGLLPFAAQAQATAEDALWRQIAGGGYVLL
                     IRHAATEPGFGDPPQFRLGDCSTQRNLSAAGRDEARRLGEMFLVYRVPVAEVRSSPWC
                     RCVDTAKLAFGKATVWDALASLYFDDRDEAARRQLVVEGARAFMARGVAGNFVLVTHN
                     FNIRSLLGVSPAQAEVIVARLTDEGLELVGRLPPP"
     gene            complement(10370..>11149)
                     /locus_tag="VIS73_05700"
     CDS             complement(10370..>11149)
                     /locus_tag="VIS73_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762680.1"
                     /translation="IARFVPKTAQFTEWDLPKRARPHGLVVDARGIVWYTGNGNGTIG
                     RLDPQTGKVSEYRVPDGGDPHTIVIAEDGTFWFTVQNGQRVGHLDPVSGRIDEYPLSG
                     NPYGLAIDRNGIVWVCRLRGDSLAWIDPKTGQRGEVDTGSGSAPRRIATAPDGNLWIA
                     YNGNGRLAKLDPAAKRIVKTWPLPSAGGYRAYAVTVDGAGRVWVNGIDTDKVSIFDPT
                     SETFRVIELPSKGVGIRKAIIDADGRYWYMGSHNGRLGVVE"
BASE COUNT         1828 a         3928 c         3567 g         1826 t
ORIGIN      
        1 ggcaatcaaa caccctgcct acccgctgct cttcgacccg cagacggccg gcggcctgct
       61 cgccagcgtg ccggcggcgc aggtcgacgc ctgcctggct gcgctgcacg aactcggcta
      121 cgcccgggcg acgcgcatcg gccgagtgct gccggccggc gacgccctcg agccgatttc
      181 gctgcgctgc taagcgggaa cgaacaccga tgagccgcag cattgatttt ttcgaaacac
      241 agtttcgccg gcagctcgcc gattccgaca tcgcgctcaa ccccttcgaa gcccaggcgc
      301 tgccctttct gcacggcgac gtgctcgact tcggctgcgg cctcggcaac ctggcgctgg
      361 ctgctgccgc gcgcggctgc cgagtactcg cgctcgacgg cgcgccgacc gcaattgccc
      421 accttcagcg gcgcgctggc caggcgggtc ttgccatcaa cgccgtcgag tctgacctgc
      481 gcgactacgt gattgcgcag gacttcgacg ccgtcgtcgc catcggaatc ctgatgttct
      541 tcgactgcgt taccgcgcaa cgccagctag cggcgctacg cgagcgcgtg cgtcccggcg
      601 gcatcgcggt cgtcaacgtg ctgacgaccg acacgacttt ccgcgacatg ttcgatcccg
      661 cttcgtactg cctgttcgaa cgcgacgcgc tgcggcctgc cttcgccggc tggtccattg
      721 aatacgaatc agccgatacc ttttccgcgc cgggcgggac gaaaaagtgc tttgccacgc
      781 tgatcgcccg ccgcacagct accggattgc cttgaaccgg caggtcaggc cactgtccat
      841 ccccggtacc tccggcatcc aagcatcatt caacccgaag gagataacga tgatccccat
      901 gcctcggctg atttcccgca gcgcccttgc gctcgccctc atgctcggcg ccgcggtggc
      961 gcaagcagat gtcctacgtg tctccgccat ccccgacgag gcgccgaccg aactgcagcg
     1021 caagttcaaa ccgctcggcg actacctcga aaaggaaacc ggcatgaagg tgcagttcac
     1081 cccggtgagc gactatgccg ccgtggtcga aggcctcgcc gcgaagaaga tcgacctcgc
     1141 ttggctcggc ggcttcacct tcgtccaggc caagctgcgc accaacggcg aagccatgcc
     1201 gatcatccag cgcgccgagg acgaagtgtt caccagcaag ttcatcgtcc cgctcgacag
     1261 cccgatcaag tccttcgccg acctcaaggg caagaccttc gccttcggct caccgtcttc
     1321 gacctccggc cacctgatgc cgcggcactt cctgatgaag gacggcattg accccgaccg
     1381 ggatttcaag gccatcgcct tctccggtgc gcatgacgcg acagtcgcct tcgtcgcctc
     1441 gggcaaggcc gaggccggcg ccctcaacgc ttcggtgatg gacaagctga tcgccaaggg
     1501 cgacgagaac gccaagcgcg tgcgcgtgct cgcgaccacg ccgccttact acgactacaa
     1561 ctggacggtc cgccccggtc ttgatccggc gttgcgcgag aagatcgccc aggctttcct
     1621 caagctcgac ccggcgaatc cggcgcacaa ggagttgatg gagctgcagc gcgcctcgaa
     1681 gttcgtcgcc accaaagcgt ccaactacga cggcatcgag ggcgcggcga agagcgccgg
     1741 gctgatcaag taatccgtca atgagcttcg agctggccgg catcggcctc acgcacggca
     1801 acggctttcg cgcgctcggc agtgtctcgc tggcgctcgc gccaggcgag cgggtcgcca
     1861 tcatcggccc ctcgggcgcc ggcaagacca cgctgctgca gctgctcggc acggcgcttc
     1921 ggcccagcga tggccggctc gaagtgctcg gcgtacgccc ctggcagctc tcgggcggcg
     1981 aactgcggcg gctgcgtacg cgcatcggca cggtccacca atcgccaccg atcccgccgc
     2041 gcctgcgcgt gatcacggcg atcctcgcgg gccgtctcgg ccgctggccg ctatggcgca
     2101 gcctaggttc tctgctctac ccggccgaca tcgccggcgc acgcgacgcg ctggcgcgct
     2161 tcgacctcgc cgaccggctg ttcgatcgct gcgaccggct ctcaggcggt cagctgcagc
     2221 gcgtcggcat cgcccgcgtg ctctaccagc aacccgacct gatcctcgcc gacgaaccag
     2281 tgtcggcgct cgacccgacg ctggccgacg ccgcaatcgg cgagctcgtt gccgaatgcg
     2341 agcggcgcgg cgccagcctc gctgcctcgc tacacgccgt cgacctggcg ctcaagtggt
     2401 ttccgcgcat cgtcggcctg cgtggcggcg aagtcgtctt cgatcgcccc accgaaacgg
     2461 tcagcgccaa gatgctgcgc gaactgtatg ccgccgaggg cggcgtgctg ccgacgcagg
     2521 ccaacgatcc cgaccgcctg cgacttgagc cggcggcact ggccgcagag gctaaggtga
     2581 tcaagctcgg tacctgtcgc ggacgctgaa atgagcaccg ccaccgcgct gccactcacc
     2641 gatcgcgacc ccgacgcacg cgcccggctg ggctgggcgc tggtcgcgct ggttctgctc
     2701 tggccgctgc tggtgctcgc cgagttcaag ccctgggcgc tgttcgacgc cagcaatctg
     2761 cgcgtcatgg gaagcttcct cgccggtttc ctgccgcccg agacctcgac cgaattcctg
     2821 ggctacctgc tcaaggcaac gctggaaaca ctggccatcg ccactgccgg catcgcgctg
     2881 gcgctgctgc tggcgatacc gctggccatc ctgtgcacgc gcgcgctctc ggtttcgctg
     2941 atcgggccgg gaccgggtca tcgcggcggc atcgcgctgc gcgcgcttgg ccgcgccttg
     3001 ctcaccttcc tgcgcgcgat tcccgagatc gtctgggcgc tgatgttcgt gcgcgtgctc
     3061 gggctgggac cggctgccgg cgtgctggcg ctggcgatca cttacggcgg catgctcggc
     3121 aaggtctata ccgagatcct cgaatccacc gacacgcggc cggcacgcac gctgctcgag
     3181 agcggcagcg gccggctggc ggcgctggcc tatggcctgc tgcccaacgc cgcgcaggaa
     3241 ctcacctcct acaccgtcta tcgctgggag tgcgcggtgc gcgcctcggt gatcatgggt
     3301 ttcgtcggcg ccggcggcct cggccagctc atggaccagt cgatgaagat gcttaacggc
     3361 ggcgaggcgg cgacgatcct cttcgtcttc ctgctgctgg tgctgctcgc cgacgcgctg
     3421 agctcggtac tgcgccgggc actggcatga gcacgacacg ccggactgcc ggcggcgcgc
     3481 gcgcgttcgg cgaatccgaa tgccggccgc gcggcgccgc cgacttctgc ctgtcctgcg
     3541 tgctcaccag cggcctgctg ctggcagccg tggtcggcag cttcgtctat ctcggcatcg
     3601 acttcggcga gctgttctcg ggcgaatcgg cggcgagcat gggccgcttc gccgcctcct
     3661 ttttcccgcc cgacctcgac ccggcctttc tctggaagac catctacggc gcgctgcaga
     3721 cctttgccgt cgccgcggtc gggacgctgc tcgcggcggg tgcgggtctg ctgctggcgc
     3781 tgccggcgtc gggccggcac gggatcgcgg cgatgcgcgt gtcgcgcttt tcgctgaacc
     3841 tgttgcgtag cgttcccgaa ctggtgtggg cgacgctgat ggtgctcgcc gccggcctcg
     3901 gccccttcgc cggcacattg gcgctggcgc tgcataccac cggggtgctc ggccggctgt
     3961 tcgccgaagc gatcgagaac gccccgaacg agcccgagca gagcctgctc gagtccggca
     4021 gcggcagcgg catcgccttc gtctacggca cgctgccgct ggtctggccg caatgcatcg
     4081 cctatgggtt gtaccggctg gaaatgaaca tccgcatggc cgccgtgctc ggtttcgtcg
     4141 gcgccggcgg gctcgggcag atgctctatt tccacctctc gatcttccag caggcgcagg
     4201 catcgaccgt gctgatcgcg atgttcgtgc tggtgctggc cgtggatttc ctcagcgacc
     4261 gtttgcgggc acggatgtca cgcacctgag tgctggcaac gccggcactc gatcaacgcc
     4321 actcgagacc gggggcggcg ctcgccgccg tgggatgtca agcgccatat gtatttaatt
     4381 gtcttgcttg caccgcggat tggccgatca tggacgctca ccccgcggag ttgtccgatt
     4441 ccattctcgt gcggaatttt tgccagagaa acgtgagccc cctcccatga tcagattccc
     4501 agccgcaaga cccggcgacc gtcggcgctg ttccggttac tacgaccgaa gattcacgac
     4561 ccgtcgcgcg aagacgcaaa cgcagccgac tcagttgatg ccgcaactga ccgtattcga
     4621 gaattccgtc cgttcgatct tcattcgcgc gaacggacag gccgcatacg actcggcctt
     4681 ccgcaaaccg ccggcgaacg tcgacgagcc cgattgatca gtgcggtcgc ctgagcaggc
     4741 gacgggtcgc gacgcgcttc aaggaggctc cgctcatcga ttgaatgcct gaacgaacgg
     4801 atgcggtaaa ctccgcggct atccgataac ccgccgccat aaagcgctca cgcccatgtc
     4861 gaatcgactg ctcaactggt tcaaattcgc cagtccgcaa agcttttatc cgctcgctgg
     4921 caagatgatc ccgtggttct gggcgctgac cgctgtgctc ggcatcagcg gcctcgtcct
     4981 cggcttcggc tacgcgccga cggacgcgca acagggcgag ggctatcgca tcatcttcct
     5041 gcacgtaccg gcctcgtgga tggcgatgtt catctacgtg gtgatggcct gttgggccgg
     5101 cctcgggctg atcctcaaca cccggctgtc gggaatgatg gcctcggcgc tggcgccgac
     5161 cggcgcgatg atggccttcc tctcgctgtg gaccggctcg ctctggggca agccgatgtg
     5221 gggcacctgg tgggtgtggg acgcgcggct gacctcatcg ctgatcctgc tcttcctcta
     5281 catcggcttc atcgcgctgc agtcggcgat caccgacgcc cgccgcgccg acaaggccgg
     5341 cgccgtgctg gcgctggttg gcgtcatcaa cgtgccgatc atctacttct cggtgaaatg
     5401 gtggaacacg ctgcaccaag gctatacggt cgcgccgcag aaggcttcga tcgacccggc
     5461 gatgctctgg ggcatgctgc tgatgtcggc tgccttctgg atgtattcga tcgcggtgac
     5521 gttgacccgc gcccgcgcca tcatcctcga acgcgaacgg cacaccgaat gggtgcgcgg
     5581 cgccgttccg gcgaaggcct gaatccgcct cccgcgtgtt tcagccagcc gcgcgcgccg
     5641 tgcgcgcggc gacgccgaga ctgaggcgca gggcgacgaa gaagctcagc aagatcgtcg
     5701 ccaccaccac cagcccggtc agcattcccg catcctcggc ccaggcgtcg atcgccggcg
     5761 agaggtactt ggccagcccg aagccggcga ccagcagtgc catcgccgcg atcgccacgc
     5821 ccatcacccg cgaggcgatt gccgcccggc gatcggcctg ccgcagcagg cgggcgatcc
     5881 acagtccgtt caacccatcg cagatcagca tgccgagcag gaaggccagg ccaagcgcga
     5941 gcccgtgccc ccagccgccg aactgctggg cggtcagggc gaacagcgcg gcctggctca
     6001 gcgtatcgaa ggacagcgcg aacagcgcac cgaccccggc gatcatcagc gggctgccgc
     6061 tgcgctgaaa gcggccgagc aggcgcgtgc gaatcccggc cggggacacc aactcgtggc
     6121 gcggcgtggt catcacggcg cgcaggttga ggaaggccag cgcaatcagg aagacgatcg
     6181 atatccaaac gccgaggtgc gccagccagg ccggcacctg ccagttggcg gcgagggcgc
     6241 tcaccgcgac cgcgacgaca acgaccaccg cgccgtggcc gagcgagaac agcacgccgc
     6301 aatgccgggc gaaggccggg cgctgtcgcg cgttgtagcg ggtcaggccg tcgatggtcg
     6361 cgagatggtc ggcgtcgaag ccatggcgcg cgccccagag gaagaccagc gccagcacgg
     6421 caagaaaatc ggtcggcagt gtttccacgc acctctcctg aacgaaccgg ctttcgctac
     6481 cggtatgaag ttttgaccga atcttaatac cgaagtcggg gcacgaaaag gggtcagccc
     6541 actgaacaag gtggccgtca tggatggtgc gcggtgataa tcggcactcg cccatcacca
     6601 gagccgtcca tgcacatcga gctcaccccg gttcaggcac gcgtcatcgg ctgcctgata
     6661 gagaaggaaa tcaccacgcc cgagcagtac ccgctgtcgc tcaacgcgct gaccaacgcc
     6721 tgcaatcaga agagcaaccg agaaccggtt ctcgaactcg acgaccggac cgtgcaggac
     6781 accgtcgatg ggctgatcaa ggtctatctg gtcagcgagc agaccggcta cggcagccgt
     6841 gtcgccaagt acaagcaccg cttctgcaac tccgactacg gcaagctgca cttcacgccg
     6901 caggaactgg cggtggtctg cgaactgctg ttgcgcggcc cgcagacacc gggcgagttg
     6961 cgcagccacg ccagccgcat ggcgacgctg cgggatgtgc acgaagtcga agcgcttctc
     7021 gaaggcctcg ccgcgcgcga ggacggcccc ttcgtcgtcc gcctggcgcg ccagcccggc
     7081 aagcgcgaag cgcgatacgc gcatctgttc tcgggtgcgg tcaccggcga tgtcgaaccc
     7141 gcagcgcacg caacggctgc tggcgactcc ggcgctcgcc tcgaagaacg cgtcgcgcaa
     7201 ctcgaggccg aagtcgccac cctgcgcgag cggctggcag tcctcgagtc gccgcccgaa
     7261 agccagcttc cctgacgccg gttcagcgcc ggccgcaagg gccggctgcc cgctgaagcc
     7321 gggcccgacc gactcagcgc ggccgtcgcg gcagaatgac gccgagcgcc agggcgaccc
     7381 agccagccat caaggcgaca ccgcccagcg gtgtcagcga tcgcagttct tgaatgccaa
     7441 agaggctgcg caggtacagg ctgcccgaga acagcacgat gccggcggcg agcagaacgg
     7501 ccgcggccgt gatccagcgc gaagccggac gcagggcgat ggccagccca acggcgacga
     7561 ggccgatcgc atgcagctcg tggtagcgcc gcgcggtttc gaaccagccg gccggatcgt
     7621 tgacgagcag caggggtttg agcgcgtgcg cgctcgcggc gccgagcgcc acggccaggg
     7681 cggcggcgag cgcgccggct gcgacggcca gccgcgccgc cagcggccag tcattcatct
     7741 cggcgccccg gcgtcagtcg gtgccagcgt ctgcgtcagc cagcggccga tgtcgacgat
     7801 ctcctcgatg cacaccgagt gcggcatcgg gtattcgtgc cagtcgacca cgtagccgcg
     7861 gcgcagcagt tcgtcgcggg cccgcgtgcc cagctttagc gagaccacgt cgtcttcgac
     7921 gccgtgtgcg gcaaacaggg ggatggttcg gttggcctcg ctggcgccgg cgaacagcgg
     7981 atcgggctcc gggatgtagg tcgacagcgc aatcacgccg gcgagcggcg ccggatgacg
     8041 gagcgcagtc gcgtaggcaa tcgcaccacc ctgcgaaaaa ccggcgatga cgattcgctc
     8101 ggtggcgatg ccgcgcgcga cctcgcgcgc gatcaatgcc tcaatcgcct gccgcgaacg
     8161 ctcgatgccc tcgccgtcga cgactcggtt gtcggggtcg agcgaaatga tgtcgtacca
     8221 cgcgggcatg acgtagccac cgttgcaggt gaccggcatc tgctgcgcgt gcggaaagac
     8281 gaaacgcagc tttagcgccg gcggcagccg caattcggga acgataggca cgaaatccga
     8341 gccgtcggcc cccagcccat gcagccagat caccgccgca tcgggcgtcg cgccggtctc
     8401 gatctcaatt gcttgcagca gttcgctcat tctcttgtcc cttggtcgag gtcccgcatt
     8461 ctgccgcatg cggcgccact gcacggactg gaatgatcgc cgcccgccct tgttggcccc
     8521 gcatggcccc ggcccgatcg acggcaggcg gcgggccgcg ctcctgagcc aatccgtacc
     8581 gtcgccaata gagaaaggca cttccatgag aagaatgctg ctgaccctgg gtctcggcct
     8641 gctgctcgcg ggctaggccc tcgccgccga ccgcgtgcaa cgctacgaca cgcgcctgga
     8701 aggcttcacg ctcgccggcg taccggtggc caccagcgcc accggcaacg cgcggggcga
     8761 ggtcatcgac agcggcagcg cactgagctt ccgtgtcaac gttgccggca tccgcaatct
     8821 gttcatggcg cacatccata tcgcgccgca accggtaacc gtgaccgacc cggccgggcc
     8881 ggtcgccttc tggttcgccc gcggtccgcc gccgcagcgg actctggccg aaatggtgaa
     8941 cggccgactc gctgaaggct tcatcatcac caacagcgac ttggtctcct gggataccgc
     9001 cgacccatta agcgggacga tcgaaggcct gatccgcgcc atcgacgaag gccgtgcctc
     9061 ggtaatcgtc cgcaccagag acgacggcgc cggtcgccgg cgattcgcca tttggcgaac
     9121 tacgcggcac cctgcgctaa gcgcgcgaca cgccaaacag aacagaagcg ccccgccgag
     9181 gcggggcgct gttcgcggcc gtggccgccg gtcagtggat cggcagacgc ttgctgccca
     9241 tcgttgcctt cttcggcagc gtcagttcca gcacgccgtc ggcaaacttg gcgtcggcct
     9301 tggcgtcgtc gacgtcctga cccaactgga aggaccgtga tacctgcccg tagtaccgct
     9361 ccgagcgcag taaccgctca ccttccttga cctcgttttc ctgcttcacc tcggccgaga
     9421 tcgacacggt actgccttcc accgtgacgt ggatgtcgtc cttcttgacc ccaggcattt
     9481 ccgcgtggac gcggtaggca tccttgtcct cccgcacgtc cagcttaatc tgctgcggcg
     9541 tcatcgcaga catcccgccc ggcaggtcga ccggcttgac gaagaacccg cggaagaggt
     9601 cttcccacgg atcgtagcgc gtcaggttgc ccatgatcgt cctcctttct tgccttgact
     9661 cgacatcggc ggaggcctcc gatgcttccg gaagtcccgc ccctcaattt ctatatatgg
     9721 cgccagcggc cagattcaag gcggcgcccg cccgtccttg accggcgtca atgtgccgcc
     9781 ggcgctcagg gcggcggcag gcgaccgacc aactcgaggc cctcgtcggt cagacgggca
     9841 acgatcacct cggcctgcgc cggcgagacg ccaagcagcg agcggatgtt gaagttgtgg
     9901 gtcacgagca caaaattgcc ggccacgccg cgggccatga acgcgcgggc accttcgacc
     9961 acaagctggc ggcgcgccgc ctcgtcgcgg tcgtcgaagt agagcgaggc aagcgcatcc
    10021 cagaccgtcg ccttgccgaa agccagcttt gccgtgtcga cgcagcggca ccagggactg
    10081 ctgcgcacct cggccaccgg tacccggtac acgagaaaca tctcgccaag gcgtcgcgcc
    10141 tcgtcgcgcc cggccgccga aagattccgt tgcgtcgaac agtcgccgag ccggaactgc
    10201 ggcggatcgc cgaagccggg ctccgtcgcc gcatggcgga tcagcagcac gtagccgccg
    10261 ccggcgatct gtcgccacag cgcatcctct gccgttgcct gcgcctgtgc cgcgaacggc
    10321 aacaggccgg cccaaagcgc gatcgctgcc cagcaggtac gcagcgcgat cactcgacaa
    10381 cccccagccg gccgttgtgg ctgcccatgt accagtagcg gccatcggca tcgatgattg
    10441 ccttgcggat gccgacgcct ttcgagggca gctcgatgac gcggaatgtc tcgctcgtcg
    10501 gatcaaagat cgagacttta tcggtgtcga tgccgttgac ccagacgcgc ccggcaccat
    10561 cgacggtgac cgcgtaggcg cgatagccgc cggcgctggg cagcggccag gtcttgacga
    10621 tgcgcttcgc cgccgggtcg agtttcgcca gccggccgtt gccgttgtag gcaatccaca
    10681 ggttgccgtc gggcgccgtc gcgatccgcc gcggcgccga accagaaccg gtatcgactt
    10741 cgccgcgctg cccggttttc ggatcgatcc atgccagcga gtcgccacgc aggcggcaga
    10801 cccagacgat gccgttacgg tcgatcgcca gcccgtaggg attgcccgac agcgggtatt
    10861 cgtcgatacg gccgctgact gggtcaaggt gaccgacgcg ctggccgttc tgaacggtaa
    10921 accagaaggt cccatcctcg gcgatcacga tcgtatgcgg atcgccacca tcgggtacgc
    10981 ggtactcgct gaccttgccg gtctgcggat cgaggcggcc gatcgtgcca ttgccgttgc
    11041 cggtgtacca gacaattccc cgtgcatcga cgaccagacc gtgcggccgg gcacgcttcg
    11101 gcaggtccca ctcggtgaac tgcgcggtct tcggcacgaa gcgggcgat
//
LOCUS       DATDAA010000172         2533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_152529,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000172 DATDAA010000000
VERSION     DATDAA010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152529"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VIS73_05705"
     CDS             <1..488
                     /locus_tag="VIS73_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024682.4"
                     /note="dGTPase family type 2 subfamily; presumably
                     hydrolyzes dGTP to deoxyguanosine and triphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEY5762681.1"
                     /translation="REQPLFESQYCEVTRRYPGLEDRRLIYEIIRRMINTVVTDLIEA
                     TKRNLDEAAPQSIDDVRAAGRPLVGFSADIGEQHRLLKQFLHQHLYSHERKLEMTRKV
                     QTVLRDLFDTFMNDVDRMPGEFAARAAEGDEDVRARVVADYIAGMTDRYAFAEHRRIS
                     G"
     gene            523..1236
                     /gene="pyrF"
                     /locus_tag="VIS73_05710"
     CDS             523..1236
                     /gene="pyrF"
                     /locus_tag="VIS73_05710"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY5762682.1"
                     /translation="MVPKDIPSKDRLIFAMDVPDCDAAMSLAEELGDAVSFYKLGLEL
                     MMSGGYFELLDWMLARDRKVFCDLKFFDIPATVGSAVRQLKDRGASFVTVHGNQSIME
                     AAAENKGGTLKVLGVTVLTSLDRGDLDDLGFDCDVGELVLSRARRALQAGCDGVISSG
                     LEVPRLREFVDEKLVVVTPGIRPVDNKPAGDQKRVVTVETAFANGADYIVVGRPIRDA
                     ASPREAAESIQASIEAQFA"
     gene            1233..2297
                     /gene="hemE"
                     /locus_tag="VIS73_05715"
     CDS             1233..2297
                     /gene="hemE"
                     /locus_tag="VIS73_05715"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019837436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY5762683.1"
                     /translation="MTKLKNDLLIRALLRKPVPRTPVWIMRQAGRYLPEYRATRAEAG
                     DFMSLCRNPELACEVTMQPLRRYQLDAAILFSDILTVPDAMGLGLHFATGEGPKFERP
                     VRSAAEIRNLPIPDVGETLGYVFDAVRTIRRELDGAVPLIGFSGSPWTVGTYMVEGGS
                     SRDFARIKGLAKDDPAALEELLAKVATITTDYLNAQIEAGAQAIQIFDTWGAALPRDD
                     YRRFSLASMQQIVDGLTRDKDGERIPVILFTKGAGEMLADMVSTGCDALGVDWTTDLE
                     TARAYVEDKVALQGNVNPATLRGSPEVIRQAVADALASYGKGPGHVFNLGHGITPDID
                     PDHLGVLLDAVHELSPQYHL"
BASE COUNT          475 a          799 c          831 g          428 t
ORIGIN      
        1 tgcgggaaca gcctctgttc gagtcacagt actgcgaggt cacgcggcgc taccccgggc
       61 tcgaggaccg ccggctgatc tacgagatca tccgccggat gatcaacacc gttgtcacag
      121 acctgatcga agcgacgaag cgcaatctcg atgaagccgc gccgcaatcc attgatgacg
      181 ttcgggccgc cggcaggccg ctggtcggct tctccgcgga catcggcgaa caacaccggc
      241 tgctgaagca gttcctgcac cagcacctgt acagccacga acgcaaactg gagatgacgc
      301 gcaaggtgca gaccgtcctg cgcgacctgt tcgacacctt catgaacgac gtcgaccgca
      361 tgcccggcga gtttgccgcc agggcggcgg aaggcgacga ggacgttcgg gcccgcgtcg
      421 tggccgacta catcgccggc atgacggatc gttacgcatt cgctgaacac aggcgaatct
      481 cgggttagaa ttcgcggccg gcaaacatcc ttggatattc acgtggtccc gaaagacatt
      541 cccagcaagg ataggctgat attcgccatg gacgtgcccg actgcgatgc ggcgatgtcg
      601 ctggcggaag aactgggcga cgcagtcagt ttctacaaac tcggtctgga gttgatgatg
      661 agcggcggct atttcgagtt gctcgactgg atgctagcgc gcgaccgcaa ggtgttctgc
      721 gatctcaagt tcttcgacat tccggcaacc gtgggatccg cggtgcggca actgaaggac
      781 cggggcgcga gtttcgtgac cgtgcacggc aaccagtcga tcatggaggc ggctgcggag
      841 aacaagggcg gcacgctcaa ggtgctcggg gtgaccgtgc tgacgagcct cgatcgcggc
      901 gatctggacg acctcggatt cgactgcgac gtcggcgaac tcgtgctgtc ccgcgcgcgg
      961 cgcgcgctcc aagccggttg tgacggcgtc atatcgtcgg gactcgaggt gccgaggctc
     1021 agggagttcg tcgacgagaa gctggtcgtc gtgacgccgg gcatacgccc ggtcgacaac
     1081 aagccggccg gcgaccagaa acgtgtggtc acggttgaga ccgcgtttgc gaacggcgcc
     1141 gactacatcg tcgttggccg tcccatccgc gatgcggcca gtccgcgcga agcggcagaa
     1201 tccatccagg catccatcga ggcacagttc gcatgaccaa gctcaagaac gatctgctca
     1261 tccgcgcgtt gctgcgcaaa cccgtaccgc gtacgccggt gtggatcatg cgccaggcag
     1321 ggcgttacct gcccgagtac cgggcaacga gggccgaggc aggcgacttc atgagcctct
     1381 gccgcaaccc cgagctcgcc tgcgaagtga cgatgcagcc gctgaggcgg taccagctcg
     1441 atgccgcgat cctgttctcc gacatcctca ccgtccccga cgcgatgggc ctcgggctcc
     1501 atttcgcaac cggcgaaggg ccgaagttcg agcgcccggt gcggtctgcc gccgagatcc
     1561 gcaatctgcc gatcccggac gtcggcgaga cgcttggtta cgtattcgat gccgtgcgca
     1621 cgatccgccg cgaactcgac ggtgcagtac cgctgatcgg tttttccggc agcccgtgga
     1681 cggtgggcac ctacatggtc gaaggcggat cgagccgcga ctttgccagg atcaaggggc
     1741 tggcgaaaga cgaccccgct gcgctcgagg agctgctcgc caaggtcgcg acgatcacga
     1801 ccgactatct caatgcccag atcgaggcgg gtgcccaggc gatacagatc ttcgacacct
     1861 ggggcgcggc gctcccgcgc gatgactacc gtcgcttctc cctcgccagt atgcagcaga
     1921 tcgtcgatgg cctgacgcgc gataaggatg gcgagcgcat cccggtcatc ctgttcacca
     1981 agggagccgg tgaaatgctt gccgacatgg tgagcacggg ctgcgatgcg ctgggcgtcg
     2041 actggaccac ggacctcgag accgcgcgcg cctatgtcga agacaaggtt gcgctgcagg
     2101 gcaacgtgaa tcccgcgacc ctgcgcgggt cgcccgaggt tatccggcag gccgtcgctg
     2161 atgcgctcgc gagctacggg aaggggcccg ggcacgtgtt caacctcggc cacggcatta
     2221 cacccgacat cgatccggac cacctgggcg tgttgctcga tgccgtccac gagctgagcc
     2281 cccagtacca cctgtagggg cggttctcga accgcggccc ggttcgcggg cccaggccga
     2341 cgcctacagg ggaattcgcg ggccctggcc cgctaccgtg ggcagcggtg gcgaaggttt
     2401 ctcagtagta gtacgtatgg ccggccgctg ccgccactct gagaatgatt ttatgagcgc
     2461 caagacggat gagaagctgt ccgtcgagaa ggcgatgtca cgcgttctca acgccgaaag
     2521 ggatgcgctg gcg
//
LOCUS       DATDAA010000173         2951 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_467912,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000173 DATDAA010000000
VERSION     DATDAA010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467912"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(471..2522)
                     /locus_tag="VIS73_05720"
     CDS             complement(471..2522)
                     /locus_tag="VIS73_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5762684.1"
                     /translation="MRDWLFNLPIGKKLWLFNLLTAMIAGVFAVALLIFVVWRIEYAS
                     AERESRIKAAIVAENALPALQFGDAMTAAEILGGLERDESVIDAFIIDAQGTPFAGFR
                     SDRTTSAVLGIEPIRVSLPIMSGNELLGTIEINNDPSEVHRQILSYVAAVAIATMVAL
                     LLGTFVVIRLQRTITSPLAQLTELMRAAAQSNEYSQRILLPRHDEIGEMSQSFNRMID
                     VIKQRERALNVELGERRRAEQQLEHLAHHDPVTGLPNRHYFRRRAAEVGADAGPSSVA
                     VLFIDIDNFKYVNDTFGHDLGDKLLVTVAERLVRSVRANDAVVRFGGDEFVVLLDQSG
                     GPEQAMKLGEKIGKAITERMSIDGNDFFVTCSIGVAVAPEHGVVLDELLTRADVAMYV
                     AKAAGKNGVRLWEADATAATSARFSIENALRQALANNELEMYYQPIVSLDGVRIVGSE
                     ALMRWRNPALGFVSPAEFIPVAEECGLIGAMGDWAMRTAFAQNAEWNRRFAPMNVSVN
                     VSGRQFNDPAFAQRAAQIAADCGLPSGRCELELTESILMSHIGEASRVLHELSDAGFS
                     IALDDFGTGYSSLAYLKRLPIDKLKIDRSFVSDLPESAEALAIAEAIVAMSTALRLSV
                     IAEGIETAQQRDALAGMRCRLGQGYLFSRPLPPEQFAELLESGLPIAATSALMSAARQ
                     G"
     gene            complement(2532..>2951)
                     /locus_tag="VIS73_05725"
     CDS             complement(2532..>2951)
                     /locus_tag="VIS73_05725"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEY5762685.1"
                     /translation="LYNFALYTEWPISPDSFEFCLVGAAGTNGVRQAADALSRRTITG
                     RTIRVRFADFGDELSTCHLALLPAASPQQVAPALARLQGEPVLTIVDGDGEVGEAAIL
                     RLMRRNERLVFEANLTAARAVGLSLSSKMLRLARAVR"
BASE COUNT          553 a          951 c          915 g          532 t
ORIGIN      
        1 ccggccgttg gcgatccggc caggatcttc cgcgcttgat cgtcttggat gaccggttta
       61 cggcggccaa cctgaacccc gccttggctc aacccggcca ggagcggccg gtcgagcgtt
      121 cccttaattc atcactcagc agtctggtcg tcgctaagag ctgccggacg acgaatgcga
      181 actgttcggc cgctagcgcc gttgggcgtg tggcctctgt cggtcggcaa cgcgctggtc
      241 aactgccgtt cagatcgaat tcacatagaa cggcagcgtc tcgcgccggc gaactcacgc
      301 tacgaaccca ccggaatcat ttcatgatca ctacgggaac agtctgttag cgacccaaat
      361 tagccacaac gttcgacttg cagaaattgg cgtcttgcac cggcgggaag cgttccgcct
      421 ccggtagttg ggtcagcgcc gggtgccgcg gatactagca ccgacgccgc ctagccctgc
      481 cgggcggcgc tcatcaacgc cgatgtcgca gcgatcggca ggccgctctc cagcagttcg
      541 gcgaactgct caggcggcag cggtcggctg aacaaatagc cttgccccaa cctgcagcgc
      601 atacccgcga gcgcgtcgcg ctgctgtgcg gtctcgatgc cctcggcaat gaccgacaac
      661 ctgagtgctg tagacatcgc cacgatcgcc tcggcgatcg ccaaggcttc ggccgactct
      721 ggcaggtcgg aaacaaagct gcggtcgatc ttcagcttgt cgatcggcaa gcgcttcagg
      781 taagcgagcg aagaatagcc agtcccgaag tcgtcgaggg cgatcgagaa gcccgcgtcg
      841 ctcagctcat ggaggacgcg cgaggcctcg ccgatgtggc tcatcaggat gctttccgtc
      901 agttccagct cgcaccggcc gctcggcagc ccgcagtcgg ccgcgatctg cgcggcgcgc
      961 tgtgcgaagg cgggatcgtt gaactgccgc ccagaaacat tgaccgagac gttcatcggc
     1021 gcgaaacgac ggttccactc agcgttctgg gcgaaggccg tgcgcatcgc ccagtcgccc
     1081 atggcgccga tcaatccgca ttcctcggcg accgggatga actcggccgg cgagacgaag
     1141 ccgagcgcgg gatttcgcca gcgcatcagc gcctcgctgc caacgattcg cacgccgtcc
     1201 agcgaaacga tcggctggta gtacatctcc aactcgttat tggccagcgc ctggcgcagt
     1261 gcgttctcga tggagaagcg cgccgaggtt gcagctgtcg catccgcttc ccagaggcgg
     1321 acaccgttct tgcccgcggc cttagcaacg tacatcgcaa catcggcacg cgtgagcagc
     1381 tcgtcgagga cgacgccgtg ctccggcgcc accgcgaccc cgatcgaaca ggtgacgaag
     1441 aagtcattgc catcgatcga catgcgctcg gtaatcgcct tgccgatctt ctcgcccagc
     1501 ttcatcgcct gttcaggacc gccgctctgg tcgagcagca ctacgaactc gtcaccgccg
     1561 aaacgtacga ccgcatcgtt ggcgcgcacc gagcgcacca accgctcagc aacggtgacc
     1621 agcaacttat caccgaggtc gtggccgaaa gtgtcgttca cgtacttgaa gttgtcgatg
     1681 tcgatgaaga gtaccgctac cgacgacggg ccggcgtctg caccgacttc ggctgcgcgc
     1741 cggcggaaat agtggcggtt gggcaggccg gtgaccgggt cgtggtgtgc caggtgttcg
     1801 agctgttgct cggcgcgccg ccgttcgccc agctcgacgt ttagcgcacg ttcacgctgc
     1861 ttgatcacat caatcatgcg gttgaagctc tggctcatct cgccgatctc gtcgtggcgc
     1921 gggagcagga tccgctgcga gtactcgttg ctttgcgcgg ccgccctcat cagttcggtc
     1981 agttgcgcga gcggactggt aatcgttcgc tggaggcgaa tcacgacgaa ggtgccaagc
     2041 aggagggcaa ccatggtcgc tattgcaacc gctgcgacgt agctgagaat ctgcctgtgc
     2101 acctcggaag gatcgttgtt gatctcgatc gttccgagca gttcattgcc actcatgatt
     2161 ggcaggctga cccgaatcgg ctcgatgccc agaaccgccg aggtcgtgcg gtcagatctg
     2221 aacccggcga atggtgttcc ctgcgcgtcg atgataaagg cgtcgatcac gctctcgtcg
     2281 cgctccaggc cgccaagaat ctcggcggct gtcattgcgt cgccaaactg aagggccggc
     2341 agcgcgttct cagcaacgat cgccgccttg atccgcgatt cgcgctcggc cgacgcgtac
     2401 tcgatgcgcc agaccacgaa gatcagcagc gccacggcaa agacgccggc gatcatggcg
     2461 gtgagcaaat tgaacagcca cagcttcttg ccaatcggca agttgaagag ccagtcgcgc
     2521 atcggcattc tctaacgcac cgctcgggct agccgtagca tcttggagct gagcgacagg
     2581 cctactgccc gcgcggcggt gaggttggct tcaaaaacaa gccgttcatt gcgccgcatg
     2641 aggcgaagaa tcgccgcttc gccaacctcg ccatcgccgt cgacgatggt cagcactggc
     2701 tcgccctgca ggcgtgccag cgccggggca acttgctgcg ggctggccgc cggcaacagc
     2761 gcgagatgac aggtcgagag ttcatcgcca aagtcggcaa agcgcacgcg gatcgttcgt
     2821 ccggtaatcg tccgccggct cagggcgtcg gcggcctggc gcaccccgtt ggtgccggca
     2881 gcgccgacca ggcaaaactc gaaagaatct ggcgagatcg gccactccgt gtagagggcg
     2941 aagttgtaga g
//
LOCUS       DATDAA010000174        15631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_118634,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000174 DATDAA010000000
VERSION     DATDAA010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 15631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15631
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118634"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VIS73_05730"
     CDS             complement(<1..221)
                     /locus_tag="VIS73_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762686.1"
                     /translation="MPEPRKFVFARRDVNALGQIFTPAVVVEAMLALRRNQGSVGAGK
                     IGAIRDALPALRVMLATGGPASGGAMDFWA"
     gene            307..1923
                     /locus_tag="VIS73_05735"
     CDS             307..1923
                     /locus_tag="VIS73_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEY5762687.1"
                     /translation="MLVAANITMQFGAKPLFENVSVKFGEGYRYGLIGANGAGKSTFM
                     KILAGILEASAGNVSKDPNERMAHLKQDQFAYEDQRVIDVVMMGHAEMWACMSEKDAI
                     YANPEASEEDYMHAAELESKFAEYDGYTAEARAGELLLGAGIPTEQHAGPMREVAPGW
                     KLRVLLAQALFANPDILLLDEPTNNLDINTIRWLENILNERNSTMIIISHDRHFLNQV
                     CTHMADLDYGTIKVYPGNYDDFMEASTLARQRQQQANAKAKERIADLQDFVRRFSANK
                     SKAKQATSRVKLIEKIRPEDVKPSSRQYPWIRFDYDEKEKLHRQAVEIENLAFAYPGG
                     ETLFEKFSTVIDASDRIAVIGENGVGKTSFLKLLMGELQPTKGTLKWAEKAKPGYYAQ
                     DHAADFANQTSLVEWIGEHVRQGGYEGDDIETLIRGTLGRLLFSGDEFDKPVNVISGG
                     EQGRMLFGKLMLKKPNVLVMDEPTNHLDMESIESLNTGLEKFAGTLIFVSHDREFVSS
                     LATRIFEIKDGRINDYRGGYEDYLAAQGLE"
     gene            complement(1950..2411)
                     /locus_tag="VIS73_05740"
     CDS             complement(1950..2411)
                     /locus_tag="VIS73_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY5762688.1"
                     /translation="MKFRCAILACVLCAASSGVLAADYIENGADIVKATDWAKMQTVE
                     VVMTEHHFEPKDLAFKVGTPYRLVLKNRGELDHYYTAAEFFKAVAWRKAQTPRPRGGE
                     LKAPYFTAVEAYKGGGTVELFFVPVKRGKYPVICTLEDHEEKGMTGSITVD"
     gene            2462..4369
                     /locus_tag="VIS73_05745"
     CDS             2462..4369
                     /locus_tag="VIS73_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5762689.1"
                     /translation="MITLRSLTFARAGEKLVDSASLQVHAGHKVGLVGANGCGKSTFL
                     ALLTDAHHAESGDLELPPGWTIAHVAQETEALDRGALDYALDGDAELRRVEAELEAAE
                     LANDGHALGSLHARFDEIGGYASRARAAGILHGLGFGEADLARPLAEFSGGWRMRLHL
                     ARALVCRSDLLLLDEPTNHLDLDAVLWLEGWLARYPGTLLMVSHDRDFLDRVCTHVIH
                     IAERQMTLYRGNYSAFERTRAERLKVQQSLYERQQREVAHLQRFIDRFRAKATKARQA
                     QSRIKALARLSEVLPAHADSPFHFEFPEPTAMPDPLLALRDAAVGYDGRAILDGISFD
                     LRPGDRIGLLGRNGAGKTTLVKLLAGQLAPLAGSRHEGKDLAIGYFAQHCVEALRPEE
                     SALWHMSRLDPKAREQDLRNFLGGFDFHGEMATRPVAPFSGGEKARLALALIVRARPA
                     LLLLDEPTNHLDLEMRESLTVALQEFEGTIVLVSHDRHLLRATADSFLLVAGGRATPF
                     DGDLDDYANWLDADRRAAREPPAASAAAPPARADRELEKADRQALLTRRRPLAKEIAR
                     LEAKLASWQGELKLLEQRLADPALYASNDPALLQTLNRRQSELTTAIDDAETRWLDAS
                     EALAALPEPDA"
     gene            4472..5080
                     /gene="thrH"
                     /locus_tag="VIS73_05750"
     CDS             4472..5080
                     /gene="thrH"
                     /locus_tag="VIS73_05750"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HEY5762690.1"
                     /translation="MQLVCLDLEGVLIPEIWIAFADRTGIAELRRTTRDEPNYDTLMK
                     YRLDILRQHKLGLPDIQQVIGGLTPEPGAREFLDALRRDYQLVILSDTFYEFAMPLMA
                     QLGFPTLFCHKLESDAQGFLVNYHLRMPNQKAEAVRRFKEINYKVIAAGDSYNDTTML
                     KEANAGILFRAPANVIAEFPQFPVETEYAALRAQIDAASARI"
     gene            5089..5964
                     /gene="purU"
                     /locus_tag="VIS73_05755"
     CDS             5089..5964
                     /gene="purU"
                     /locus_tag="VIS73_05755"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEY5762691.1"
                     /translation="MHRERFYTLSASCPDTTGIVARVASFIAGHGGWITESSYHAESP
                     DGNGGSGRYFMRIEMRADSLPFHLAEFRSRFAPLAGELAMDWRISDSAVKKRVVILVS
                     QQEHCLYDLLSRWQSKELDIEIPCVISNHMSFKGFVEWHGIPFHHVPVAADNKAKTFA
                     EIRRLFDESRADVMVLARYMQILPPELCAAYPGQVINIHHSFLPSFVGARPYHQAYTR
                     GVKLIGATCHYVTENLDQGPIIEQDVIRIDHSDAVEDMVRYGKDIEKAVLARGLRYHL
                     EDRVLVHGNKTIVFR"
     gene            complement(5992..6978)
                     /locus_tag="VIS73_05760"
     CDS             complement(5992..6978)
                     /locus_tag="VIS73_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762692.1"
                     /translation="MASVTRLPMLPDDGEVPESTRRIIDQHERVHYCGYWVKTYPVPP
                     DSLQAKRFLIEALARRLFNHTEHGLNMPGSRLDEARAAYEGEADPALKRVKGGMLAGA
                     LFNRAIDIFRRLVELQSDGVAIGSDYSLVRECGECLKKAIALGQLVLHRSGDEGIDEL
                     WGEPFRVFSLNLEDYFESRYIKIAQSMRDIDRIASSMSECIGGQALFADLPPHIEAFA
                     RAAKAKVETLRTDPDIFEVWPALVTAGERLVRFVPNLGLVEAVPERDGGGPARLAQTI
                     ADAQRLISAGRELIFYVVRARTPMPKSTAEFIARCEYFRDSGSLPVPPWATA"
     gene            complement(7136..7834)
                     /locus_tag="VIS73_05765"
     CDS             complement(7136..7834)
                     /locus_tag="VIS73_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEY5762693.1"
                     /translation="MKFSRRFSNRLHGEPVGADTVVVVDVLRSFTTAGVALAGGAAAI
                     YPVPGRAAMERLRRSLSGALAVGSLPGGDPAPGFDVGNSPAALSRIDLRGRAVIISTA
                     AGVTGLLHHRQAPRLYAACLGNARATASAIKAAGAGEVCFVITGEWVDRDGDEDIACA
                     DYIEALLRGHPVSATDYERRVRHSDFGRRFAAGDHAQLPIADLDCCAQADCFTFAMPV
                     RHDGDRPVIVGDAR"
     gene            complement(7926..8750)
                     /locus_tag="VIS73_05770"
     CDS             complement(7926..8750)
                     /locus_tag="VIS73_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEY5762694.1"
                     /translation="MLIAQLSDPHILAAGQLLYRRFDSAAALRDAVSRIGKIKPHPDC
                     VVVSGDLVNHGAAAEYAQLRDLLAPLTMPVFLMPGNHDERGALATAFAGDDRADKLRQ
                     AFTGAPLCCQRVDLAPADGEVLTLLLLDTVIPGREGGEVGAAQLDWLEANCPADAPTM
                     LFLHHPPFSTGIAGMDAITCSGAGLLADWLAAHRNVIALSSGHVHRTIFTRFAGIAAC
                     IAPSTALQIALDLGGKAAALSWTPEPGGFLLHHWHEQSLVTHLVPSAAVEVVRYGA"
     gene            complement(8760..12410)
                     /locus_tag="VIS73_05775"
     CDS             complement(8760..12410)
                     /locus_tag="VIS73_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5762695.1"
                     /translation="MARSLRFWLALGLSSLVALAIGLVAAALLTVLLPRLNEQVESAQ
                     NAVGVALAGQIADYLDASVAELERVAADIAREPVSGSPRRSVALDTLAQARADLNILY
                     LLDKRGVAIDVGLPIDRRGLRNELLGLDFSARDFVGNVQRSGRSAWSDAFLSPRGMIT
                     VAAAVPIPAGRVSTGLLLVGEFDLEVLSQHFSDLARDADVFGLIVDAKGYLVAHPDAS
                     RAIRHESFRGLPPASSGGEPRSARRFVMDGVEHLGTAIRVDGPEWTVVIAQPIDKAFA
                     TVRSTLWAVALGSVAALLLALVTAFIYGRRLVGRVREFGEHAQAVADGDYSAPLPFSR
                     AEELNVLAASMRRMANAILEREEALAASESRYRDLFDKAPLAYQSLSMDAELIEVNDA
                     WLALTGYSRDEVVGRPIADFVEQESLPVLAENFPKFIAAGTIDGPVFNIRRKDGGSRT
                     IVINGRIHHDADGTAKTHCILTDITERNRLELAKQQAEATLRRQAQRAVAMLDLPTAA
                     AELDEQAFLQHGLGVVERLTGSTISFVHFVDADQTTLSFGTWSRATLDHYCKAVENEH
                     SPIAEAGIWADALRTRAPVVYNDYPSAAGRKGLPPGHSPLHRLVSVPVIEADKVRMLL
                     GIGNKAEPYEHFDVETARLISQDIWRQVSRQRADAAQRLAASVFSASAEGICITDAEK
                     RIVSVNPALMSITGYRAEEVLGQTPRVFSSGRHDGEFYREMWARIEAHGVWRGEIWNR
                     RSNGEVYPEWLTITAVHDQTGEVINYIGSFFDISERKRAEEDIRFLAHHDSLTKLPNR
                     TLLDDRVRQAIAKSRRNQDHTAVLFLDLDRFKLINDTLGHDVGDRLLERVADRLRGVL
                     RETETVARLGGDEFIIVIPELADIDRVAQVAQKVIDVVSEPQEIDARVLHVTPSIGIS
                     VYPDDGQDAATLLRNADTAMYHAKERGRNNFQFFTSAMNIAVQERVAIEEELRGALSR
                     NELRLHYQPQVDVRSGQVTGMEALIRWQHRERGLVPPDRFIPIAEETGLIVSIGEWVL
                     QEACRQGKRWHDAGHKHLRVGVNLSARQFQQAGLREQIADALADSGMAPQALEVELTE
                     SMLMADPQAATDLLQDLATLGIRMAIDDFGTGYSSLAYLKRFPVARLKIDRSFVRDIS
                     TDPNDAAIVRAVVAMADSLRMAVIAEGVETAEQLKFLERHGCFEVQGYFFSRPEPADK
                     FANFRFSLPAAH"
     gene            complement(12460..13590)
                     /locus_tag="VIS73_05780"
     CDS             complement(12460..13590)
                     /locus_tag="VIS73_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5762696.1"
                     /translation="MGRIEAKRALLWTGAVALVLLLVGCAPPEPLRVGFLGGVSGRVA
                     DLGIAGRDAALLAVEMRNARGGVDGRPVELLIEDDGQDPERAREALARLIARNVAAVV
                     GPMTSAIALAVVPQANAARLTLVSPTVTTNALTGLDDHFFRVLAPTAAHVAKSADYYF
                     HERGLHRLALIYDLRNKAYTESWASDFRAAFVAAGGVIVDEERFVSGEAVHFTELAAQ
                     LLDRSPDGVIVIANSVDTALLIQKIRQRDAAVAIGTSEWAATERLIELGGRAVEGVVV
                     SQYLDRDSREPQYVGFREAYRSRFGREPGYPGLTAFDATNVVLDALDSRQSGQSLKQA
                     ILARREFAGAQGAIRFDAAGDSGRGTFLSVIRDGDFQPLGPS"
     gene            complement(13834..15198)
                     /locus_tag="VIS73_05785"
     CDS             complement(13834..15198)
                     /locus_tag="VIS73_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY5762697.1"
                     /translation="MKKLLVTLAMLVAVSAPVVAQEAAPAVDVVAEAATTAAEVAAPA
                     IEVNKGDVAWMMTATLLVILMALPGLALFYGGLVRAKNMLSVLMQVMVVFSLIGVLWA
                     VYGYSLAFGGEGLIVGTLDKLFLSGVTVDSLADTFTDNVKLPEYVFIAFQATFAGITC
                     ALIVGSFAERIKFSAVLLFSVLWFTFCYIPIAHMVWGAGGYLLDKGALDFAGGTVVHI
                     NAGVAGLVGAYVIGKRVGYGKEALTPHSLTMTMIGASLLWVGWFGFNAGSNLEATAGA
                     ALAFINTLVATAAAVLAWAIGEAMFKGKASMLGAASGAVAGLVAITPACGSVGPMGAI
                     AIGFAAGFVCLWGVNGLKKMLGADDSLDVFGVHGIGGILGALLTGVFTAPSLGGTGGD
                     DFSIASQVIIQAEGVGITILWSAVVAFIAYKIVDVVIGLRVPEDEEREGLDITAHGET
                     AYHM"
     gene            complement(15213..15551)
                     /gene="glnK"
                     /locus_tag="VIS73_05790"
     CDS             complement(15213..15551)
                     /gene="glnK"
                     /locus_tag="VIS73_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY5762698.1"
                     /translation="MKLVTAIIKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEAAVKDDVLDQVIEAIEKSAATGKIGDGKIFVFDLQQVVRI
                     RTGETGAEAL"
BASE COUNT         2904 a         5273 c         4944 g         2510 t
ORIGIN      
        1 gcccagaagt ccatggcgcc gccggaggcc ggcccgccgg tggccagcat cacccgaagc
       61 gccggcagcg cgtcgcggat ggccccgatc tttccggccc ccacgctgcc ctggttgcgg
      121 cgcaaggcca gcatcgcctc gaccaccacg gcgggcgtga agatctggcc gagtgcgttc
      181 acatcgcggc gtgcgaagac aaatttcctg ggttctggca tgcgttcggg cagggtccgg
      241 cacggagtct ggcgattcgg gcgggtagaa tagcgcccct ttggcctcat cgctccaccc
      301 gctcccatgc tcgtcgccgc caacatcacc atgcagttcg gggccaagcc ccttttcgaa
      361 aacgtctcgg tcaagttcgg cgagggctat cgctacggcc tgatcggcgc caacggcgcc
      421 ggtaagtcga ccttcatgaa gattctcgcc ggcattctcg aagcctcggc cggcaacgtc
      481 tcgaaggacc ccaacgagcg catggcgcac ctcaagcagg accagttcgc ctacgaggac
      541 cagcgcgtca tcgacgtggt gatgatgggg cacgccgaga tgtgggcgtg catgagcgaa
      601 aaggacgcga tctacgccaa cccggaggcg agcgaggaag actacatgca cgccgccgag
      661 ctcgagagca agttcgccga gtacgacggc tacaccgccg aggcgcgcgc cggcgagctg
      721 ctgctcggcg ccggcatccc aaccgaacag cacgccgggc cgatgcgcga ggtggcgccg
      781 ggctggaagc tgcgcgtgct gctggcgcag gcgttgtttg ccaaccccga tatcctgctg
      841 ctcgacgagc cgaccaacaa cctcgacatc aacaccatcc gctggctcga gaacatcctt
      901 aacgagcgca acagcacgat gatcatcatc agccacgacc ggcacttcct gaaccaagtg
      961 tgcacgcaca tggccgacct cgactacggc acgatcaagg tctacccggg caactacgac
     1021 gacttcatgg aagcctcgac acttgctcgc caacgccagc agcaggccaa cgccaaggcc
     1081 aaggaacgca tcgccgacct gcaggacttc gtccgccgct tctcggccaa caagtctaag
     1141 gccaagcagg cgaccagccg cgtcaagctg atcgagaaga tccgacccga agacgtcaag
     1201 ccgagttcgc gccagtaccc gtggatccgc ttcgactacg acgagaagga gaagctgcac
     1261 cgccaggccg tcgaaatcga gaacctcgcc ttcgcctacc cgggcggcga aaccctgttc
     1321 gagaagttct cgacggtgat cgatgccagc gaccggatcg cagtcatcgg tgagaacggc
     1381 gtcggcaaga cgagcttcct caagctattg atgggcgaac tgcagccgac caagggaacg
     1441 ctgaaatggg ccgagaaggc caagcccggc tactacgcgc aggatcacgc cgccgatttc
     1501 gccaaccaga cctcgctggt cgagtggatc ggcgagcacg tgcggcaggg cggctacgag
     1561 ggtgatgaca tcgagaccct gatccgcggc acgctcggcc gcctgctgtt ctcgggcgac
     1621 gaattcgaca agccggtcaa cgtcatctcg ggcggcgagc aggggcgcat gctgttcggc
     1681 aagctgatgc tgaagaagcc aaacgtgctg gtgatggacg agccaaccaa ccacctcgac
     1741 atggaatcga tcgagtctct gaacaccggg ctggaaaaat tcgccggcac gctgatcttc
     1801 gtttcgcacg accgcgagtt cgtctcctcg ctggcgacgc gcatcttcga gatcaaggac
     1861 ggccgtatca acgactaccg cggcggctac gaggactatc tcgcggcgca gggtctggag
     1921 tagccaggcc ttacgccaga tgtggaaggt cagtcaacgg tgatactgcc ggtcatgccc
     1981 ttctcctcgt ggtcttcaag cgtgcagatc accgggtact tccctcgctt gacgggtacg
     2041 aagaacaact cgaccgtgcc accgcccttg tacgcctcga ctgccgtgaa atacggtgcc
     2101 ttcagctctc caccccgcgg tctcggagtc tgcgccttgc gccaagcgac cgccttgaag
     2161 aattccgcgg cagtgtagta atggtccagt tcgcctcggt tcttgaggac caatcggtat
     2221 ggggttccga ctttgaacgc gagatccttg ggttcgaaat gatgctcggt catcacgact
     2281 tcaaccgtct gcatcttggc ccagtcggtc gccttgacga tatctgcacc gttctcgatg
     2341 tagtctgcgg cgaggacgcc gctgcttgca gcacacaaca cgcacgcgag aatggcgcac
     2401 ctgaatttca tcatgagacc cccctgttcg tttttcgcac tcgacgcagt atagacaaac
     2461 cgtgatcaca ctgcgcagcc tcaccttcgc gcgcgccggc gagaagctcg tcgacagcgc
     2521 cagcctgcag gtgcacgccg ggcacaaggt cggcctggtc ggcgccaatg gctgcggcaa
     2581 gtcgaccttc ctcgcgctgc tcaccgacgc ccaccacgcc gaatcgggcg atctcgagct
     2641 gccgccgggc tggacaatcg cccacgtcgc gcaggaaaca gaggcgctag accgcggtgc
     2701 gctcgactac gcgcttgacg gcgacgcgga actgcgccgg gtcgaggccg aactcgaagc
     2761 ggccgagctg gccaacgacg gccacgcgtt aggcagcctg cacgcgcgtt tcgacgagat
     2821 cggcggttac gcatcgcgcg cccgcgccgc cggtatcctg catggactcg gcttcggcga
     2881 ggccgacctg gcgcggccgc tcgccgagtt ctcgggcggc tggcgcatgc gcctgcacct
     2941 cgcccgcgcg ctggtctgtc ggtcggacct gctgctgctc gacgagccaa ccaaccacct
     3001 cgacctcgac gccgtgctct ggctcgaagg ctggctggca cgctatccgg gaaccctctt
     3061 gatggtttcg cacgaccgcg attttctcga tcgcgtgtgc acccatgtga tccacatcgc
     3121 cgagcggcag atgacgctct accgcggcaa ctactcggcc ttcgagcgca ctcgcgccga
     3181 gcggctgaag gtgcagcagt cgctctacga acggcagcag cgcgaggtcg cgcacctgca
     3241 gcgcttcatc gatcgctttc gcgccaaggc aaccaaggcg cgccaggcgc agagccggat
     3301 caaagcgctg gcgcggctct ccgaagtgct gccggcgcac gccgacagcc ccttccattt
     3361 cgaatttccc gaaccgacgg cgatgcccga tccgctgctg gcgctgcgcg atgcggcggt
     3421 cggctacgac ggccgggcta tcctcgacgg catctccttc gatctgcgcc ccggcgaccg
     3481 catcggcctg ctcggccgca acggcgccgg caagacgaca ctggtgaagc tgctcgccgg
     3541 gcagctggcg ccgctcgccg gcagccgtca cgaaggcaag gatctggcga tcggctactt
     3601 tgcgcagcac tgcgtcgagg cactgcggcc cgaggagtcg gcactttggc acatgagccg
     3661 gctcgacccg aaggcgcgcg agcaggacct gcgcaacttc ctgggcggct tcgattttca
     3721 cggcgagatg gcgacgcgtc cggtcgcgcc attttccggc ggcgaaaagg cgcgcctcgc
     3781 cctggcgcta atcgttcgcg cgcgaccggc gctcctgctg ctcgacgagc cgaccaacca
     3841 cctcgatctc gaaatgcgcg agtcgctgac cgtcgcccta caggagttcg agggcacgat
     3901 cgtcctcgtc tcgcacgacc ggcacctgct gcgcgccacc gccgacagct tcctgctcgt
     3961 tgccggcggc cgcgcgacgc ccttcgacgg cgacctcgac gactacgcga actggctcga
     4021 cgccgatcgg cgcgccgcac gcgaaccccc ggctgcctct gcagcggcac cgcccgcgcg
     4081 cgccgatcgc gagctggaga aggccgaccg ccaggcgctg ctgacccgac ggcggccgct
     4141 ggccaaggaa atcgcccggc tcgaggcaaa actggcgagc tggcagggcg agctgaaact
     4201 gctcgagcag cggctcgccg atccggcgct ctacgcgagc aacgaccccg cgttgttgca
     4261 gacgctgaac cgccgccagt ccgaactgac gacggcgatc gacgacgccg aaacgcgctg
     4321 gctcgacgcc tcggaggcac tcgcggcgct gccagaaccc gacgcctgac gaggccgaca
     4381 ccgctgcccg gcggcacggg cggctgatag aattcgcgcc tttcgcaacc gccccgccgt
     4441 cggcgcgcgg gcgcccaccg gagcctgatc cgtgcaactc gtctgcctcg acctcgaagg
     4501 ggtgctgatc cccgaaatct ggatcgcctt cgccgaccgt accggcatcg ccgaactgcg
     4561 ccgcacgacg cgcgacgaac cgaattacga cacgctgatg aagtaccggc tggacatcct
     4621 ccgccagcac aagctcggcc tgcccgacat ccagcaggtg atcggcgggc tcacgccgga
     4681 gcccggcgcg cgagaatttc tcgatgcgct gcgccgcgac taccagttgg tcatcctctc
     4741 cgacaccttt tacgagttcg cgatgccgct gatggcccaa ctgggctttc cgacgctgtt
     4801 ctgtcacaag ctcgagtccg acgcgcaggg tttcctggtc aactaccacc tgcgcatgcc
     4861 caaccagaag gccgaggcgg tgcgccgctt taaggaaatc aattacaaag tgattgccgc
     4921 cggcgactcg tacaacgaca cgacgatgct caaggaggcg aacgccggca tcctcttccg
     4981 tgcgccggcg aacgtcatcg ccgagtttcc gcagttcccg gtcgagaccg agtacgctgc
     5041 cctgcgcgcg cagatcgacg cggcgtcggc gcgcatctag ctgcgaccat gcaccgcgaa
     5101 cggttctaca cgctctccgc ctcctgcccc gacaccacgg gaatcgtcgc ccgtgtcgcc
     5161 agcttcatcg ccggacacgg cggctggata accgaatcga gctatcacgc cgaaagcccg
     5221 gacggcaacg gcggcagcgg tcgctacttc atgcgcatcg agatgcgcgc cgattcgctg
     5281 cccttccatc tcgccgaatt ccgcagccgc tttgcgccgc tcgccgggga actggcgatg
     5341 gactggcgaa tcagcgattc ggcggtgaag aagcgggtcg tcatcctcgt ttcgcagcag
     5401 gaacactgcc tctacgacct gctctcgcgc tggcaaagca aggaactcga catcgagatt
     5461 ccctgcgtga tctccaacca catgagcttc aaaggcttcg tcgagtggca cggcatcccg
     5521 ttccaccacg tcccggtcgc cgccgacaac aaggccaaga ccttcgccga aatccgccgg
     5581 cttttcgacg aatcacgtgc tgatgtgatg gtactggcgc gctacatgca gatcctgccg
     5641 cccgagctgt gcgccgccta cccgggtcag gtcatcaaca tccaccacag cttcctgccc
     5701 agcttcgtcg gcgcgcgccc ctaccatcag gcctatacgc gcggcgtcaa gctgatcggc
     5761 gcgacctgcc actacgtcac cgaaaacctc gaccaagggc cgatcatcga gcaggatgta
     5821 atccgcatcg accactccga cgcggtcgag gacatggtcc gctacggcaa ggacatcgaa
     5881 aaggccgtgc tggcgcgagg cctgcgctac cacctcgagg accgcgtcct cgtccacggc
     5941 aacaagacga tcgtctttcg ctaaaccgca ggcgcgggcg cggcaagccg ctcaggcggt
     6001 cgcccagggc ggcaccggca ggcttccaga atcgcggaag tattcgcagc gcgcgatgaa
     6061 ctcggcggtg ctcttcggca tcggcgttcg cgcgcgaacg acgtagaaga tcaactcgcg
     6121 cccggcgctg atcagtcgct gggcgtcagc aatcgtctgg gcaagacggg cggggccgcc
     6181 cccgtcccgc tcgggcacgg cttctaccag ccctagattc ggcacgaagc gcaccagtcg
     6241 ttcgccggcg gttaccagcg ccggccagac ctcgaagatg tccggatcgg tgcgcagggt
     6301 ttcaaccttg gccttcgcgg cccgcgcgaa ggcttcgata tgcggcggca ggtcggcgaa
     6361 caaggcctgc ccgccgatgc actcgctcat gctcgaggcg atgcggtcaa tgtcgcgcat
     6421 cgactgggcg atcttgatgt agcggctttc gaagtagtcc tcgagattca acgaaaacac
     6481 ccggaatggt tcgccccaga gctcgtcgat cccctcgtcg ccgctgcggt ggagaaccag
     6541 ttgcccaagc gcgatggctt ttttcaggca ctcgccgcat tcgcggacca gcgaatagtc
     6601 ggacccgatc gccacgccat cggactgcag ttcgacgagg cgacgaaaga tgtcgatggc
     6661 gcggttaaac agcgcgccgg ccagcatccc acccttgacc cgcttcaatg ccggatctgc
     6721 ctcgccttcg taggctgccc gcgcctcgtc cagcctcgat cccggcatgt tcaggccgtg
     6781 ctcggtgtgg ttgaacaggc ggcgcgccag cgcctcgatc aggaagcgct tggcctgcag
     6841 cgaatcgggc ggtaccggat aggtcttaac ccagtagccg cagtaatgga cgcgctcgtg
     6901 ctgatcgatg atgcgccggg tggattcggg cacttcgccg tcatcgggca gcatgggaag
     6961 gcgggtaacg gatgccattg gaaggcctct aacgactgga tgcgcagtgc agcaccataa
     7021 tccttgctcc gatgacgatc tgcaagcgat cgttgcccaa gcgcaatggc cgcccgcggc
     7081 ctgcgcgaga tccgcgtcca caaccgctgg cgatcgcacc ccttggccgg ttcgatcatc
     7141 gcgcgtcgcc aacgatgacc ggtcggtcgc cgtcgtgacg gaccggcatg gcgaaggtaa
     7201 agcagtcggc ctgcgcgcag caatcgaggt cggcaatcgg cagttgcgcg tgatcgccag
     7261 cggcgaagcg gcgcccgaaa tcggagtggc gcacgcgtcg ctcgtagtcg gtggcggaga
     7321 caggatgacc gcgcagcagc gcctcgatgt agtcggcgca ggcgatgtcc tcgtcgccgt
     7381 cgcgatcgac ccactcgccg gtgatgacga agcatacctc tccggctccg gccgccttga
     7441 tcgccgacgc cgttgcccgc gcgttgccga gacaggcggc gtacaggcgc ggcgcctgtc
     7501 gatggtgcag cagcccggtc acgccggccg ccgtgctgat gatcaccgcc cggccacgca
     7561 ggtcgattcg cgacagcgcc gcaggcgagt tgccaacatc gaaacccggc gccgggtcgc
     7621 ctccgggcaa tgagcccacc gccaacgcac cactcagcga gcgacgcaac cgttccatcg
     7681 ccgcccgccc gggcaccgga tagatggctg cggcaccgcc ggcaagtgcc acgccggcgg
     7741 tcgtgaacga gcgcaacacg tcgaccacga ccaccgtatc ggcgccgacc ggctcgccgt
     7801 gcagacggtt cgagaagcgg cgcgagaact tcatcgccgg tcaaccggtt cggctcgcgc
     7861 accgcgcgca tcggcccaac gccatcgtcc gcgagacgcg ccggcggtca gcagcatcct
     7921 ggtgctcatg cgccgtagcg aacgacttcg accgccgcgc tcggcaccag atgggtcacc
     7981 agcgactgct cgtgccagtg atgcaagagg aagccgccgg gctcgggagt ccacgacaga
     8041 gccgcggcct tgccgccgag atcgagggcg atctgtagtg ccgtcgacgg cgcgatgcac
     8101 gccgcgatgc cggcaaaccg cgtgaagatg gtgcggtgga cgtggccgct cgacagggca
     8161 atgacattgc ggtgcgcggc cagccagtca gcgagcaacc cggcgccact gcaggtgatc
     8221 gcatccatgc cggcaatgcc ggtggaaaag ggcgggtggt gaaggaagag catcgtcggc
     8281 gcgtcggccg ggcagttcgc ctcgagccaa tctagctgcg ccgcgccgac ctcgccgcct
     8341 tcgcggcccg gaattactgt gtcgagcagt agcaaggtca gtacctcgcc gtcggcggga
     8401 gcgaggtcga ctcgctggca gcacagcggc gcgcccgtga atgcctgccg cagcttgtcg
     8461 gccctgtcgt cgccggcgaa ggccgtggcc agcgcaccgc gttcgtcatg attgcccggc
     8521 atcaggaaga ccggcatcgt cagcggcgcg agcaagtcgc gcagctgcgc gtattcggcg
     8581 gcagctccgt ggttgaccaa atcaccactg acgacgacgc aatcggggtg cggcttgatc
     8641 ttgccgatgc gactcaccgc atcgcgcagc gcagcagcgc tgtcgaaacg gcgatagaga
     8701 agctggccag cggcaaggat atgcggatcg gaaagctgcg cgatcagcat ggcgggcgtt
     8761 cagtgcgccg caggcagcga gaagcgaaaa ttcgcgaact tgtccgcggg ctccggacgg
     8821 ctgaagaagt agccctgcac ctcgaagcag ccgtgccgct cgaggaactt gagctgctcg
     8881 gcggtctcga cgccttcggc gatcacagcc atgcgcaggc tgtcggccat cgccacgacg
     8941 gcgcggacaa ttgcggcatc gttgggatcg gtggagatat cgcgaacgaa ggagcggtcg
     9001 atcttcagtc gcgccaccgg aaagcgcttg aggtaggcga ggctcgaata gccggtaccg
     9061 aagtcgtcga tcgccatacg gatgccgagc gtagcgagat cctggagcag gtcggtcgcc
     9121 gcctgcgggt cggccatcag catgctctcg gtcagctcga cctcaagcgc ctgcggcgcc
     9181 atcccgctgt cggcaagcgc gtcggcgatc tgctcgcgca aacccgcctg ctggaactgc
     9241 cgcgccgaca gattgacgcc tacacgcagg tgcttatggc cggcgtcatg ccagcgtttg
     9301 ccctggcggc atgcctcctg caacacccat tcgccgatac tgacgatcaa cccggtttcc
     9361 tcggcgatcg ggatgaagcg gtcgggcggt accagcccgc gctcgcgatg ctgccagcga
     9421 atcagcgcct ccataccggt cacctggccg ctgcggacat cgacctgcgg ctggtagtgc
     9481 agcctgagtt cgttacgcga cagcgcgcca cgcagttcct cctcgatcgc cacacgctcc
     9541 tgcaccgcga tgttcatcgc cgatgtgaag aactggaagt tgtttcgccc gcgctccttg
     9601 gcgtggtaca tcgcggtgtc ggcgttgcgc agcagcgtcg ctgcatcctg cccgtcatcg
     9661 ggatagacgc tgatgccgat gctcggcgtc acgtgcagca cgcgcgcgtc gatttcctgc
     9721 ggctcggaaa cgacatcgat gaccttctgc gccacctgcg ccacccggtc gatgtcggcg
     9781 agttcgggga tgacgatgat gaattcgtcg ccgccgagtc tggccacggt ttcggtctcg
     9841 cgcagcacgc cgcgcaggcg gtcggcgacg cgctcgagaa ggcggtcccc gacgtcgtga
     9901 ccgagcgtgt cattgatcag cttgaaacgg tcaagatcga ggaacagcac cgccgtgtgg
     9961 tcctgattgc gacgcgactt ggcgatcgcc tgacgaacgc ggtcgtcgag cagtgtgcga
    10021 ttgggcagct tggtcagcga gtcgtggtga gcaaggaagc gtatgtcctc ctcggcacgc
    10081 ttgcgctcgc tgatgtcgaa gaagctgccg atgtagttga ttacctcgcc ggtctggtcg
    10141 tgaaccgcgg taatggtcag ccattcggga tagacctcgc cgttgctgcg gcgattccag
    10201 atctcgccgc gccagacgcc gtgcgcttca atccgcgccc acatctcgcg gtaaaactca
    10261 ccgtcatggc ggcccgagga gaacacccgt ggcgtctgcc ccagaacctc ttcggcgcga
    10321 tagccggtaa tgctcatcag cgccggattg accgagacga tccgcttttc ggcgtcggtg
    10381 atgcagatgc cttcggcact cgccgaaaaa actgacgccg cgaggcgctg tgcggcgtcg
    10441 gcacgctggc gagaaacctg ccgccagatg tcctgactga tcagccgcgc ggtttcgacg
    10501 tcgaagtgct cgtagggctc ggccttgttt ccgatgccga gcagcatgcg caccttgtcc
    10561 gcctcgatca ccggaacgct gaccagccgg tgcagcggcg agtgcccggg cggcagcccc
    10621 ttgcgaccgg cggcgctcgg gtaatcgttg tagacgacgg gggcgcgcgt acgcagcgcg
    10681 tcggcccaga tgccggcctc ggcaattgga gagtgctcgt tctcgacggc cttgcagtaa
    10741 tgatcaagtg tcgcccgcga ccaggtgcca aagctcagcg tggtctggtc ggcatcgacg
    10801 aaatggacga aggagatcgt gctcccggtc agccgctcga ccacccccag tccgtgctgc
    10861 agaaaggcct gctcgtcgag ttcggcggcc gccgtcggca ggtcgagcat ggcgacggcc
    10921 cgctgcgcct gccgccgcag ggtcgcttca gcctgctgtt tcgccaactc gagacgattc
    10981 cgctcggtta tgtcggtaag gatgcaatgg gtctttgcgg tcccgtcggc atcgtggtgg
    11041 attcggccgt tgatgacgat cgtccggctc ccgccatcct tgcgccggat attgaaaacc
    11101 ggtccgtcga tcgtgcccgc ggcgatgaac ttgggaaagt tctcggcgag caccggcaac
    11161 gattcctgct caacaaagtc ggcgatcggc cgcccgacga cttcgtctcg cgagtatccg
    11221 gtcagtgcca gccaggcgtc gttgacctca attaactccg catccatcga caacgactga
    11281 taggcgagcg gcgccttgtc gaagaggtct cggtagcgcg actcggaggc ggccagcgct
    11341 tcctcgcgtt cgaggatggc attggccatg cggcgcatgc tcgccgctag cacgttgagt
    11401 tcctcggcgc gcgaaaacgg cagcggcgca ctgtaatcgc cgtcggcgac ggcctgggca
    11461 tgttcgccga attcacgaac ccggccaacc agccgacggc cgtagatgaa agccgtcacc
    11521 aatgccagca acagcgccgc tacgctgccg agcgcgactg cccaaagcgt cgagcggacg
    11581 gtggcaaaag ccttgtctat cggttgcgcg atcaccaccg tccactccgg cccgtcgacc
    11641 cgaatggcgg tgccgagatg ctcgacgccg tccattacga aacggcgcgc gctacgaggc
    11701 tcgcccccgc tggatgccgg cggcaatccg cgaaagcttt cgtggcggat cgctcgactg
    11761 gcatcagggt gggcaacgag ataccccttc gcatcaacga tcagcccgaa gacatccgca
    11821 tcccgtgcca gatcggagaa atgctgagac aagacttcga gatcgaactc gccgaccagc
    11881 aacagcccag tggatacgcg gccggccgga atcggcaccg cggcagcgac cgtgatcatg
    11941 ccgcgcggcg agaggaaggc atcggaccag gccgaccggc ctgatcgctg tacgttaccg
    12001 acaaagtcgc gcgccgaaaa atcgagtccg agtagttcgt tgcgaagacc gcgccgatcg
    12061 atcggcaggc cgacatcgat ggcaacaccg cgcttatcga gcaggtaaag gatgttgaga
    12121 tcggcccgcg cctgcgccag cgtgtccagc gccacgcttc ttcttggcga tccggaaacc
    12181 ggctcgcggg cgatatcggc ggcaacgcgt tccagttctg ccaccgatgc gtcgaggtaa
    12241 tcggcgatct gccccgcgag cgcaacgccg actgcgttct gcgccgattc gacctgttcg
    12301 tttagccgcg gcagcagaac cgtcagcaag gcagcggcca caaggccgat agccagcgca
    12361 accagcgaag atagacccaa cgccagccag aagcggagag agcgtgccat cggccgatgc
    12421 taaaccgaaa cgccgccggt gtccggcctt ggccgcgact caggacgggc cgagcggttg
    12481 gaaatcgcca tcgcggatga ccgagagaaa ggtgccgcgt ccggaatcgc cggctgcgtc
    12541 aaaacgaatt gccccctgcg cgccggcaaa ctcgcggcgc gcgaggatcg cctgcttgag
    12601 cgattgcccg gactggcggg agtcgagtgc atcgagcacg acgttggtcg cgtcaaaggc
    12661 ggtcaggccg ggataacccg gctcgcgccc gaagcggctg cgatacgcct cgcgaaagcc
    12721 aacgtactgt ggctccctgc tgtcgcgatc gaggtactgg gataccacga cgccttcgac
    12781 ggcgcggcca ccgagttcaa tcagcctctc ggttgccgcc cactctgagg tgccgatcgc
    12841 gacggcagcg tcgcgctggc ggatcttctg gatcagcagt gccgtatcaa ccgaattggc
    12901 gatcacgatc acgccgtcgg gcgaacggtc gagaagctgt gcggcaagct cggtgaaatg
    12961 gactgcctcg ccggaaacaa agcgctcttc gtcgacgatc acgccgccgg ctgcgacaaa
    13021 cgcggctcgg aaatcgctcg cccagctctc ggtgtaagcc ttgttacgca gatcgtagat
    13081 caatgccaga cgatgcagcc cgcgctcgtg gaagtagtag tcggcagatt tcgcaacatg
    13141 ggcggcggtc ggcgcaagca cgcggaagaa gtggtcgtcg aggccggtca gggcgttggt
    13201 cgtcaccgtc ggcgaaacca gcgtcagccg ggcggcgttg gcctgcggca cgacagctaa
    13261 tgcaatcgcg ctggtcatcg ggccgacgac cgcggcgacg ttgcgggcga tcagccgcgc
    13321 tagcgcctcc cgtgcccgct cggggtcctg gccgtcgtcc tcgatcagca gttcgaccgg
    13381 cctgccgtcg actccgccgc gcgcattgcg catctcgacg gcgagcagcg cggcgtcacg
    13441 gccagcgatg ccaagatcgg caacgcgccc cgacacccca ccgaggaagc cgacgcgtaa
    13501 cggctcgggt ggcgcgcagc cgacgagcag cagcacgagt gcgacggcac ccgtccacaa
    13561 caaagccctc ttcgcctcaa tgcggcccac ctccatcccc ctcagcgcag cccatcgtca
    13621 aggctcttga ttttcgctcg attctaaggg gattcccgcc gatctcgcta gctcgccgat
    13681 taagccaaag gcaatagtgc gcaaagacgc caaaccagcc cccgcccata aaaaaaatgg
    13741 ggcgtcccgg aggacgcccc aaacctgtca gtgcaacagg gaggaggaga ctgcagggcg
    13801 gcacgccttc cagcccgccg cccggtgaag caattacatg tggtacgcgg tctcgccgtg
    13861 cgccgtgatg tcgaggcctt cccgctcctc gtcttccggc acgcgcaggc caatcaccac
    13921 atcgacgatc ttgtaggcaa tgaaggcaac gaccgccgac cagagaatcg tgatgccgac
    13981 gccctcggcc tgaatgatca cctgcgaggc gatcgagaaa tcgtcgccac cagtaccgcc
    14041 cagcgagggt gcggtgaaca cgccggtcag tagggcaccg aggatgccgc cgatgccgtg
    14101 cacgccgaat acgtcgagcg agtcatcggc accgagcatc ttcttcaggc cattgacgcc
    14161 ccagaggcag acgaagcccg cggcgaaacc gattgcgatg gcgcccatcg ggccgaccga
    14221 gccgcaggcc ggggtgatcg ccacgagacc ggcgacggca cccgacgcgg caccgagcat
    14281 cgacgccttg cccttaaaca tcgcttcgcc gatggcccaa gccaggaccg cggcagctgt
    14341 cgccaccagc gtgttaatga aggccagcgc agcaccggca gttgcctcaa ggttggagcc
    14401 ggcgttgaag ccgaaccagc ccacccacag gagcgaggca ccgatcatgg tcatcgtcag
    14461 cgagtgcggg gtcagcgcct ccttgccgta gccgacccgc ttgccgatca cgtaggcacc
    14521 gaccaggcca gcaacaccgg cgttgatgtg caccacggta ccgccggcga agtcgagcgc
    14581 gcccttgtcg agcaggtagc cgccagcgcc ccagaccatg tgtgcgatcg ggatgtaaca
    14641 gaaggtgaac cacagcaccg agaacagcag taccgccgag aacttgatgc gctcggcgaa
    14701 ggagccgacg atcagcgcac aggtgatgcc ggcgaaggtc gcctggaagg caataaagac
    14761 gtactcaggc agcttcacgt tgtcggtgaa ggtatcggcc agcgaatcga cggtgacacc
    14821 cgacaggaac agcttgtcga gcgtgccgac gatcagcccc tcgccaccga aagccaagct
    14881 gtagccgtag acggcccaga gcacaccgat cagcgagaag acgaccatca cctgcatcag
    14941 caccgagagc atgttcttgg cgcggaccaa gccgccgtag aacagtgcca ggccgggcag
    15001 ggccatcaga attaccagca gcgtcgcggt catcatccag gcgacgtcac ccttgttgac
    15061 ctcgatcgcc ggcgcggcga cttcagccgc ggtggtggca gcttctgcga cgacgtcaac
    15121 agcgggcgcc gcttcctggg cgacgaccgg cgccgagacg gcaaccagca tcgcgagcgt
    15181 tactaacagt tttttcatca tgaccgttcc cctcagagcg cttcggcgcc ggtttcaccg
    15241 gtgcggatgc ggacgacctg ctggaggtcg aagacgaaga tcttgccgtc gccgatcttg
    15301 ccggtggcgg ccgatttctc gattgcctcg atgacctgat cgaggacgtc gtccttgact
    15361 gccgcctcga tcttgacctt gggcaggaag tcgacgacgt attcggcacc ccggtacagc
    15421 tcggtgtggc ccttctgccg cccgaagccc ttgacctcgg tgacggtgat gccctgtacg
    15481 ccaatcgcgg acagcgcctc acgcacctcg tcgagcttga acggcttgat gattgcggtt
    15541 acgagtttca tgtctggctc cttgtcgggg cctcgcggcc ccggtttggg aatggcttag
    15601 aaggacttgc tgaccgagac gatcacgcgg t
//
LOCUS       DATDAA010000175        19349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_502610,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000175 DATDAA010000000
VERSION     DATDAA010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 19349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19349
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502610"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VIS73_05795"
     CDS             complement(<1..507)
                     /locus_tag="VIS73_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY5762699.1"
                     /translation="MGLRAGIDLGGSKIEIVVLDNEGWPRHRQRVATPQGDYPATVGA
                     VAMLVEAAEATLGARLSVGVGHPGSVSPLSGLIRNANSTCLNGQPLRRDLQTLLGREL
                     RLANDADCFALSETRDGAAAGAHSVFGVILGTGVGGGLVIDGRLLAGCNGIAGEWGHN
                     RLDVRAIDG"
     gene            591..1766
                     /locus_tag="VIS73_05800"
     CDS             591..1766
                     /locus_tag="VIS73_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762700.1"
                     /translation="MKWVIRFSLLLLFQHAATAALAESPAGAALSAVGSLASDPAPAE
                     PEAVPPQSPETASAAAPADQADAAAASEPAGEPAVETVALPPAAQPEQCVNPPSDVSP
                     WGWTRDRLFDLVCGTAFWLDSRFGDDPFDGSEEKIRGYFALQTERREGAPFETKPRFR
                     VRMDLPNLNRRLNLTVERDDERQVVTGQTGETAPALESAIAARDDATTVSLGYEARRA
                     IDRLLDFRLGIRANQGQPNPYVRSRYRREYARDENSVWRFSQSIFYRHVEGFGETTLL
                     DYEFALSEHWLFRWSNSGTVSQVTDGMAWQTAGDLAHALSPSDSLRLTLAWVGESAAP
                     VRLANYGYRLSYRRLLGRPWLVGELYAGHDFSRSDPAIARDVVAYGGASLEVHFGYF"
     gene            1782..2351
                     /locus_tag="VIS73_05805"
     CDS             1782..2351
                     /locus_tag="VIS73_05805"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEY5762701.1"
                     /translation="MRRLIRRWLPSPSQVREGRSLRWLAPLLSRPWLWQIDRRGVALG
                     AAVGVFFGFLIPIAQIPLSAIGVLILRANLPVAVVSTLISNPLTYAPIFILAHRLGRA
                     ILGEPTANGAAAAVIEGGVWTSLGELGAPLFLGLGVFAVCGSVLTWSLIQVLWLAGTA
                     LRVQRLRSRRARRRERGSVGVSDPRQNER"
     gene            complement(2323..3510)
                     /locus_tag="VIS73_05810"
     CDS             complement(2323..3510)
                     /locus_tag="VIS73_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5762702.1"
                     /translation="MKPVLKKLAYTAVALAASAGLATLFVPDPLPVDTTTVTRGALQV
                     TIDEDGETRAHDRYEVSAPVAGRVARIELHEGDLVGEGQVVAELRPLPLSARERAEQL
                     AQIAGAEARHREADAQASRTEAELKRLHQERERADALSAGGFVSPQALEQAQLAEAAT
                     EKALAAAQFHQRAAAAELRAARAARLALDSRAGSAAAVKLRSPAKGYALRIPERSERV
                     VAAGEPLVTIGDPSALEVVVDVLSSEAVRLRPGMTVLLGGWGGPEQLHGRVRVVEPYA
                     FTKTSALGVEEQRVNVVADLLDPPGALGDGYRVEARFVIWEDEDVLKIPGSALFRLGD
                     GWATFVVADGRARLRRLDIGQRGGSEAEIRSGVEAGEIVIVHPANDIKDGSRVTVRSG
                     AGR"
     gene            complement(3518..5884)
                     /locus_tag="VIS73_05815"
     CDS             complement(3518..5884)
                     /locus_tag="VIS73_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5762703.1"
                     /translation="MVAARQRMLLRDLWHLRGQVIAAALVVMCGVASFVAMRCTYDSL
                     RIAQSDYYRNYRFADVFTQLKRAPRSLLPQIGSLPGVAAAAGRVVMGVTLDVPGLGEP
                     ANGRLVSLPETAAPTLNDLYLELGRRPDPQRPSEIVASKAFARANGLAPGDTLGAVVN
                     GRWRRLQIVGIGHSPEFLYEVGGGALLPDNRRFGVLWMGEKALASAFNLEGAFNDLAI
                     TLTADAVEADVIARLDALLGRYGGLGAYGRGEHVSHTFISDEIAQNRVSSVWIPGIFL
                     AVAAFLLHIVLSRLVALQRTEIGLLKAFGYSNLTVGLHYLKLAAVTVTAGLLVGGLVG
                     LWLGEGLTVLYQDYYRFPQLPLVVTPEVVVLGLAVSLGAAGLGAVSAVRQAVRLPPAV
                     AMRAPAPASFHAGLIEHAGVLRLAPASLRMILRSLVRRRWKSLLSVVAVGCAAGILII
                     GGFFLDAMQVMIRVQFEDIQREDVLVLFNEPASAPARHEIRRLPGVLQAETFRSVPVR
                     LRHEHREKRVELLGLPPSGELRRVLDAGQRPVALPDNGLLLTRRLGETLGVGAGDSLL
                     VEVLEGARPSHRLPVAGLVDEMVGLGAYAEITLINRLMREGPSISGARLKVDPRESDR
                     LNAALKALPAVAGTAFRDAMLKSFDEIMDRSLETSTLINIVFACIIAFGVIYNGARIA
                     LSERGTELASLRVLGFTRGEVTLFLLGEQAILTLAAIPAGFALGVGASALLSAALNTE
                     LYRVPLVFSPSTFGVALLTIVTAAIISGALVARRIKHFDLIAVLKTRE"
     gene            complement(5878..6603)
                     /locus_tag="VIS73_05820"
     CDS             complement(5878..6603)
                     /locus_tag="VIS73_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5762704.1"
                     /translation="MATGAQQGNAVFRVRQVSKVYRMGEVEVHALRGVDLELYAGELM
                     VLLGASGSGKSTLLNILGGLDAPTSGEVDYLDHRLSTADETALTRFRREHVGFVFQFY
                     NLIPSLTARENVALVTEIAEQPMDPREALERVGLGDRLDHFPAQLSGGEQQRVAIARA
                     IAKRPDVLLCDEPTGALDYATGRIVLEALAAVNAELGTTLAVITHNAAIAAMADRVVR
                     MSSGTIAGIDYNSHRLTPAELSW"
     gene            complement(6669..7043)
                     /locus_tag="VIS73_05825"
     CDS             complement(6669..7043)
                     /locus_tag="VIS73_05825"
                     /inference="COORDINATES: protein motif:HMM:NF009316.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit G"
                     /protein_id="HEY5762705.1"
                     /translation="MPPAIDILVAILLLLGAGFTLLGSIGLVRFSDVLLRLHGPTKAG
                     TLGVGCVLIASALYFSSRGEGVSVHELLITLFIFLTAPVSAQLMAKCALFLARTRDRP
                     EPPGPAVAADTDRGQGSQDSNA"
     gene            complement(7043..7321)
                     /locus_tag="VIS73_05830"
     CDS             complement(7043..7321)
                     /locus_tag="VIS73_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HEY5762706.1"
                     /translation="MSGVTESVLPIVLGMLVLSMLLCGWRLARGPTRVDRILALDTLY
                     INTVALLVVGGIATDSALYFEGALVIALLGFVGTVALTKYLLNGEVID"
     gene            complement(7318..7833)
                     /locus_tag="VIS73_05835"
     CDS             complement(7318..7833)
                     /locus_tag="VIS73_05835"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEY5762707.1"
                     /translation="MTRWFPAPFLSLALAALWLVLNNTLAAGQLLLALLVGWAVPLLF
                     VSLGGGPGRERPLAKHAPWLALRLLGRLLLDIVTANLSVAAKILFVREGALSPGLVKV
                     ALRLDSPRAVAALAGIVTLTPGTLSADIVEDSATSRYQLLVHALDAPDPAALADEIRQ
                     RYEDLLLEMLR"
     gene            complement(7830..9359)
                     /locus_tag="VIS73_05840"
     CDS             complement(7830..9359)
                     /locus_tag="VIS73_05840"
                     /inference="COORDINATES: protein motif:HMM:NF009309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HEY5762708.1"
                     /translation="MSGLLVAPVVLPIVAAATMVLLRRLPPTARHGFGLIATLAFIGI
                     ALVLFGRAADGTTETLLLGNWRAPFGIVLVLDRLSAWMLLLTAFVGAATLIAAIAGGH
                     ARKLHFHPLFQIQLAGVAGAFLTGDLFNLFVCFEVLLAASYGLLLYSGERQTRRASLH
                     YITFNLVGSLLFLIAAGLIYGVTGTLSFAELASIGSQLPEADVPLLRAAMLLLLVVFS
                     IKAAALPLGLWLPATYGAADAPVAALFALLTKVGIYAVIRVFVGVFGAMEPSTPWIYL
                     AGAIPWVGALTLLFGAFGALAARHLRGIAASLILASAGTLLIAAGLPGGVAAAMYYAA
                     HSTLAGAALFLVADAIRRGRGEASDRLEPAMAVGRAGLLGGMFFVVAVAIAGLPPLTG
                     FIGKAMLLAEVFAQPGRAHLAVAVLLGSLTTLLALARAGTLLFWQTTPHRADEHVRRG
                     GRGDLWGAWLLLGGLVLLTVAAGPAQRYLSALAEQVASTRIVAAGVLGTLPRPQGARQ
                     P"
     gene            complement(9356..9724)
                     /locus_tag="VIS73_05845"
     CDS             complement(9356..9724)
                     /locus_tag="VIS73_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HEY5762709.1"
                     /translation="MALLLATGIGVLIGCGVFLVLRARSFDVVLGLTLMAYGVNLFLF
                     SMGRLGGAAAILVGGEAGQYTDPLPQALTLTAIVIGFAMTALLLAVALADFVAEKTDH
                     VDGEPGERRLRHDAEPEDEQ"
     gene            complement(9724..12546)
                     /locus_tag="VIS73_05850"
     CDS             complement(9724..12546)
                     /locus_tag="VIS73_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HEY5762710.1"
                     /translation="MTPDGLLPFVTLLPFAAAIAAGSVRSRAAAALIAGLAAAAVFAY
                     ALAVAPAIFDGTVLRWQVAWMETPAVAFGFRVDGLAWLFVSLIGGIGALVVLYAFYYL
                     DASEPAPRFYAMLLGFMGAMLGVVTADNLLLLVVFWELTSLSSFLLIGFWQHRQDARQ
                     GARMALAITGAGGLCLLAGVLLIGHIVGSFGLDEVLAAREVLQAHPAFPAALLLVLLG
                     AFTKSAQFPFHFWLPHAMAAPTPVSAYLHSATMVKAGVFLLARLYPALAGNEIWFWTV
                     STVGMATLLLGAFVAIFQHDVKGLLAYSTISHLGLITLLLGLDSPLAAVAAIFHIMNH
                     ATFKASLFMAAGIIDHECGTRDMRRINGLWRFMPYTATLAMTAAAAMAGVPLLNGFIS
                     KEMFFAETLYLEGHQLLEYITPAAATLAGGFAIAYSLRFVHDVFFNGEPVDLPRTPHE
                     PPLWMKVPVGLLVALCLVVGLAPELAVGEILAVSAGPLLPGGMPEYDLAIWHGFNWPL
                     AMSFLAMTAGAALYFGLQRLFRLHAQITQTRGKQAFDSTLSRFARFAAEISQFRNGSL
                     QRYLTLLFLVILAAGGLPFLLGPYTLGPLPLTAPDAVELTIWLVGVGAALGAVALHRR
                     RATALTFVGAVGIVVALSFEHLAAPDLMLTQLLVEVVTVVLMMLALHHLPQESVPARA
                     PAETRQLLRDGAIALAVGVGIALLALAVMTRTPAGLFPRESLADFYLSRAVPEGGGTN
                     VVNVILVDFRGFDTFGEITVLAVAALTIVALLRHLHPPPAALTHPEAVDPVRHPLLLR
                     VVSRLLLPLAAVVALYFFLRGHNLPGGGFIAGLVYAIGVVTLYLARGRGQVESRLAAD
                     WQGWLGWGLLIAGGSGAAAALFGYPFLTSTFGHPVLPVIGELPLASAALFDLGVFAVV
                     VGATLAAIVALGRLRTVGSHGTGGGG"
     gene            12847..14451
                     /locus_tag="VIS73_05855"
     CDS             12847..14451
                     /locus_tag="VIS73_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEY5762711.1"
                     /translation="MTIARLLACLLIALAAAPLSAQAQCRADAVSALPMPAATWLRSW
                     TGPLASPARLDVDAAGRVLVADPARGRLVRRAADGSLIEQIATGGQPSAVAIDPAGGF
                     WVGDAGKGTVTRHDVDGQARFALGVGDGEFGQPGDIAVDPADGSIYVSDTARQQVSVY
                     SPGGVRLRSIGTAAPADDLPAPGGQFRTPTGIVIAGDELLVGDQLNFRIQAFDKQSGA
                     FRYCLGTFRASSFFSPNSGPARSFGTVQGLWVDALGRLYVVDAYQGQVVVVDRSNGAI
                     IGRIGSFGDEPGALRVPADVVIDGNGRLFVSNGDAGRLEIFGLDGFVDLEAVVPGHVQ
                     IEPARIDRRAPPGALALAVEAPGYRSIDIDPDDVTVNGVAAQVLEVADADGNGVPELR
                     LTLRTADLLPTLADGEAVAVSVSAPLGTLRLVANTSVAVIGDLPGDSDGDGIDDFRDR
                     CPASAAGAVVDRQGCAIAQRCQCDGSGSDKRKHDDDDRHRHAHGAYVRCVEKAAAELR
                     SAGRITQQAFRSLVRDAAQSSCGRTR"
     gene            14448..16871
                     /locus_tag="VIS73_05860"
     CDS             14448..16871
                     /locus_tag="VIS73_05860"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY5762712.1"
                     /translation="MSRLASLFAAALLAGATMQALALDPPHDASRNIQCITCHMLHGS
                     PGGTLNRVAGNANVCLSCHVPTGQAAARPFAAAGQALPPEPRPTGWTAGSGISHRWDS
                     GPSGHVKPVSGNTSTGHIASLGSFTGRIEETYTITIATAGTVGTATFDWSSLRDGSGS
                     AVVTAPSVGIGSKGLSLAFANGSGTPSFRSGDQWLLRARTDLRLPDQFDVFERQLWQR
                     VMREELPDRTTLGDPKVVCSTCHDQHSQKHQPFDAAAPIFLGAGTGWISTGIGRHLQR
                     QPNENNQMCKVCHSARNVASAAAGSHPVGVAIPGGDFQSPAGVPLSAELRVECMSCHA
                     PHYTDSGGANAGNGDGFLLRTRINDLCFECHTNADRANGAHFNTTTGALWGGSKEANG
                     TQFPRHQAGMRGACVNCHWPHGWPDRNAPAQDYPRLWVERYDVDRTTKTDGADGELLC
                     FACHTAPSGGVPATPATSNIQAEFARGSALASATGDVFRHPVNDVEQQNGNNGLRVVE
                     CVDCHNPHKARANDRHAGVSGVNLAGTTLPAGSSLQQQEVCFKCHGDTFNSARPNASN
                     KRLDFNTSAANSGYHPVTQAGRGQSANLAAQLLGGLTTASTIRCSDCHNSSAFSGAVG
                     TIADSPAVTVGPHGSEFAPILRADFGRNYTSTGWNNANATLCFGCHDQTMLLARDRGS
                     GARTNFYGGGRDNLHWYHLENKGVTSSCMSCHFDLHSNRTASNTQYRVVDGATQTYLG
                     TAPPPNVKTHLVNFAPDVTGASFALPRWQINTANGVRTCDLACHGDGGKMDPVSYGPT
                     TGDETSHTY"
     gene            complement(16914..17459)
                     /locus_tag="VIS73_05865"
     CDS             complement(16914..17459)
                     /locus_tag="VIS73_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEY5762713.1"
                     /translation="MALKSTVFKVDLQLADLDRHHYADYPLTIARHPSETDERMMIRL
                     LAFALFASERLRFGRGLSDEDEPALVEDDLTGRITHWIEVGLPDERRITRACGRADRV
                     SVIAYGGNAARMWWQGIENRLGRQKKLRVLALDPEESQAMAAMAARTMRLSATIQDGT
                     LLFGDGASALNLHPRALLGAD"
     gene            complement(17526..18875)
                     /locus_tag="VIS73_05870"
     CDS             complement(17526..18875)
                     /locus_tag="VIS73_05870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5762714.1"
                     /translation="MAPPETRGIPARLFARLVDTRGDPAAGFEQSAREAIPGNFFRAI
                     GATFLVQLGDVLSGPKITLAWLLGAVGAPSYLIGLLVPIRESGSMLPQFAIAPFVQRR
                     RIRKWVWVAGSLAQALAVAGIGCAILLFDGAVAGWLIVVLLCLFSIARAFSSIAAKDV
                     LGRTIPKRWRGQTTAWGASAAGLVTLATGLVLALTPTGSGDIRLYALIVFAAAVLWLL
                     AAAVYAGVHELPDQPAGRHAVSSDALATLRLLSTDPLLRRFVIARCLLLSTALSAPFY
                     ISLAQTGHESTASLLGAFIVASGLASLLSSPFWGRFADRSSRKTMSLGAVLASATGIA
                     VFAIDRVNPTMFEAVWLLPTLSFCLAIAHQGARIGRKTYVVDMATGNRRTDYVAASNT
                     AVGLVLLIAGLLSAAGSVLSTSGVILALSLLGLAGAWRAYRLPEALADELSESHSSG"
     gene            complement(18875..>19349)
                     /gene="acnA"
                     /locus_tag="VIS73_05875"
     CDS             complement(18875..>19349)
                     /gene="acnA"
                     /locus_tag="VIS73_05875"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361476.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEY5762715.1"
                     /translation="GGVTRRNGETMPIYDAAMAYIAEGTPTVVFGGEEYGTGSSRDWA
                     AKGTQLLGVRAVIAQSFERIHRANLIGMGVLPLQFADGDSVDSLGITGEESYAIEGVG
                     NAPTPRQQATLVITRADGSSKRVTLILRIDTPIEVAYLRHGGILPFVLRQLLRPA"
BASE COUNT         3316 a         6522 c         6706 g         2805 t
ORIGIN      
        1 gccgtcgatg gcgcgcacgt cgagccggtt gtgcccccac tcgccggcga tgccgttgca
       61 gccggcgagc agtcgtccat cgatgaccag tccgccgccg acgccggtgc cgaggatgac
      121 gccgaagacg ctgtgcgcgc cggctgctgc cccatcgcgg gtctcggaca gcgcaaagca
      181 gtcggcatcg ttggccagcc gcagttcgcg accgagcagc gtctgtaggt cgcgccgcaa
      241 cggctgcccg ttgagacagg tggagttggc gttgcggatc aggccgctga gcggcgagac
      301 cgagcccgga tgtccgacgc cgacggagag ccgcgccccg agcgtggctt cggccgcttc
      361 gaccagcatg gcgaccgcgc ccaccgtcgc cgggtagtcc ccctgcgggg tggcgacacg
      421 ctggcgatgg cgtggccagc cttcgttgtc gagaacgacg atctcgatct tgctgccgcc
      481 gagatcgatt ccggcgcgaa gtcccatgct gcctttcccc tgggagtgag cgccgcattg
      541 tgccgttttc ggcgtgttcg cggcgagggc tcgcgttaca ctcccgcgcg atgaaatggg
      601 tgatccgatt ctccttgctg ctgctgttcc agcacgccgc cacggccgcg cttgccgaat
      661 cgccggccgg cgcggcgttg tccgctgttg gatcgctggc atcggacccg gcacccgccg
      721 agccggaagc ggtgccgcca caatcaccgg aaacggcaag cgcggcagca cccgctgacc
      781 aggccgacgc cgccgcggca agcgaaccgg cgggcgagcc cgcggtagaa accgtcgcgc
      841 tgccgccggc ggctcagccc gagcagtgcg tcaatccgcc cagcgacgta tcgccctggg
      901 gctggacgcg cgaccgcttg ttcgatctgg tctgcggcac ggccttctgg ctcgacagtc
      961 gcttcggcga cgaccccttc gatggctcgg aggagaagat ccgcggctat ttcgcgctgc
     1021 agaccgaaag gcgggaaggg gcacccttcg agaccaagcc gcgcttccgt gtgcgcatgg
     1081 acctacccaa cctcaaccgc cggctcaatc tcaccgtcga gcgcgacgac gagcgccagg
     1141 ttgtcaccgg gcagaccggg gaaaccgcgc cggcgctcga gtcggcgatt gccgcgcgcg
     1201 acgacgcgac gacggtctcg cttggctacg aagcgcgccg cgccatcgac cgtttgcttg
     1261 atttccgcct cggcatccgc gccaaccaag ggcagcccaa cccttacgtg cgctcgcgct
     1321 accgccgcga atacgcgcgc gacgagaaca gcgtctggcg cttctcgcag agcatcttct
     1381 atcgccacgt cgagggcttc ggtgaaacca cgctgctcga ctacgagttc gcgctaagtg
     1441 aacactggct gtttcgctgg tcgaacagcg gcacggtgtc gcaggtcacc gacggcatgg
     1501 cctggcagac tgccggcgat ctcgcccacg cgctgtcgcc cagcgacagc ctgcgcctga
     1561 cgctggcctg ggtcggtgaa agcgcggcgc cggtgcgcct tgccaactac ggttaccggc
     1621 tgagttaccg caggctgctc ggtcgcccgt ggctggtcgg cgagttgtac gccgggcacg
     1681 atttcagtcg cagcgatccg gcgatcgcgc gcgatgtcgt cgcctacggc ggtgccagcc
     1741 ttgaggtgca ttttggttac ttttgaacgc ggccagaacc catgcgccgc ctgatccgcc
     1801 gctggcttcc ttcgccgagt caggttcgag aggggcgcag cttgcgctgg ctggcgccgc
     1861 tcctgtcgcg gccctggctg tggcagattg accgccgcgg agtggcgctg ggggcggccg
     1921 tcggggtctt cttcggtttc ctgattccga tcgcgcagat tccgctgtcg gcgatcggcg
     1981 tactgatcct gcgcgccaac ctgccggtcg ccgtggtcag cacgctgatt tccaatccgc
     2041 tgacctacgc gccgatcttt atccttgccc accgccttgg ccgcgccatc ctcggcgaac
     2101 ccaccgccaa cggcgcggcg gccgcggtaa tcgagggtgg agtctggact tcgctcggcg
     2161 agctaggcgc gccgcttttt ctcggcttgg gcgtcttcgc ggtctgcggc agcgtcctga
     2221 cctggtcgct gatccaggtt ctatggctcg ccggaaccgc gcttcgcgtg cagcggctcc
     2281 gcagtcgccg tgcgagacgg cgcgaacgcg gcagcgtcgg cgtcagcgac ccgcgccaga
     2341 acgaacggta acgcgggagc cgtccttaat gtcgttggcc gggtgaacga tgacgatctc
     2401 gccagcttcg acaccactgc ggatttccgc ctcgctgccg ccgcgctgac ctatgtcgag
     2461 gcgacgcagg cgggcgcggc cgtcggcgac gacaaaggtc gcccagccgt cgccgaggcg
     2521 gaacagtgcg ctgcccggaa tcttcagcac gtcctcgtcc tcccagatga cgaatcgggc
     2581 ctcaacgcgg tagccgtcgc ccagtgcccc cggaggatca agcagatcgg cgacgacgtt
     2641 gacgcgctgc tcttcgaccc cgagggcaga ggtcttggtg aaggcataag gctcgacgac
     2701 gcgcacccgg ccgtgcagtt gctcggggcc gccccatccg ccaagcagga ccgtcatccc
     2761 cgggcgcagg cgcacagctt ccgatgagag tacgtcgacg acgacttcga gtgccgatgg
     2821 gtcgcctatt gtcaccaatg gctcgccagc cgcaaccacc cgctcgctgc gttcgggaat
     2881 acgcaaggcg tagccttttg cgggtgagcg cagcttgacg gctgccgccg aaccggcgcg
     2941 cgaatcgagc gccagtcgcg cggcccgcgc cgcgcgcaat tcggccgctg ctgcccgctg
     3001 gtggaactgc gctgccgcca gcgccttctc ggtggccgct tcggcaagct gtgcctgttc
     3061 gagcgcttgc ggcgaaacga agccgccggc cgacagggcg tcggcgcgct ctctttcctg
     3121 gtgaagccgc ttgagttcgg cctcggtccg actagcctgc gcgtcggcct cgcggtgtct
     3181 cgcctcggcg ccggcgatct gcgccagctg ctcggcgcgc tcacgcgccg acaggggcag
     3241 cggccgcagt tcggcgacca cctggccctc gccgactagg tcaccttcgt gcagttcgat
     3301 acgggcgacg cggccggcga ccggcgcgct gacttcgtag cggtcgtggg cgcgcgtttc
     3361 gccgtcttca tcgatggtca cctgcaaggc accgcgcgtg accgtggtcg tatcaaccgg
     3421 cagcggatct gggacgaaga gcgtcgccag gccagcgctg gcggccagcg caacagcggt
     3481 gtaggcaagc tttttcagga cgggtttcat ggtggactca ctcgcgtgtc ttgaggactg
     3541 cgatcagatc gaaatgtttg atgcgtcttg ccaccagcgc gcccgagatg atcgcggcag
     3601 tgacgatggt gagcagcgcg acaccgaacg tgctcggcga aaagaccagc ggcacgcggt
     3661 acagctcggt gttcagcgct gccgagagca gcgcgctggc accgacgccg agtgcgaatc
     3721 cggcaggtat cgcggcgagc gtcaggattg cctgttcgcc gagcaggaac aacgtcacct
     3781 cgccgcgagt gaagccgagt acgcgcagtg aggcgagttc ggtgccgcgt tcggagagcg
     3841 cgatgcgcgc gccgttgtag atgacgccga aggcgatgat gcaggcgaag acgatgttga
     3901 tcagcgtcga ggtctccagg ctgcgatcca tgatctcgtc gaagctcttc agcatcgcgt
     3961 cgcggaaggc ggtgccggca acggccggca gggccttgag cgcggcgttg aggcggtcgc
     4021 tctcacgggg gtcgaccttc agtcgcgcac cggagatcga cggcccctcg cgcatcaatc
     4081 ggttgatcag cgtgatctcg gcgtaggcgc caaggccgac catctcgtcg accagtccgg
     4141 ccaccggcag gcggtggctc ggtcgcgcac cctccagcac ttcgaccagc agcgaatcgc
     4201 cggctccgac tcccagcgtc tccccaagcc tgcgcgttag cagcaggccg ttatcgggca
     4261 gcgctaccgg cctttgcccg gcgtcgagta cgcgccgcag ttcgccggat ggcggcaggc
     4321 ccagcagttc gacgcgtttc tcgcggtgtt cgtggcggag ccggaccggc acgctgcgaa
     4381 aggtctcggc ttggaggacg cccggcaacc ggcggatttc gtgccgggcc ggtgccgatg
     4441 ccggctcgtt gaacagcacc agcacgtctt cgcgctggat gtcttcgaac tgcacgcgaa
     4501 tcatcacttg cattgcatcg agaaagaagc cgccgataat caggattccc gcggcgcagc
     4561 cgacggcgac gaccgagagc agcgatttcc agcgtcgccg cacgaggctg cgcaggatca
     4621 tgcgcagcga tgccggcgcc agccgcaaca cgcccgcgtg ttcgatcagc cccgcgtgga
     4681 aactcgccgg cgccggcgcg cgcatcgcca ctgcgggcgg caggcgcacg gcctggcgga
     4741 cagccgatac cgcgccgagg ccggccgcgc ctaggctgac ggccagacct aggacgacaa
     4801 cctccggcgt gaccaccagc ggcagctgcg gaaagcggta gtagtcctga tagagaaccg
     4861 tcaggccttc accgagccat agaccgacga ggccgccgac caacaagccg gccgttacgg
     4921 tgactgccgc cagtttgagg tagtggaggc cgacggtcag attggaatag ccaaaggcct
     4981 tgagcagccc gatctcggta cgctgcagtg cgaccagccg agacagcacg atgtgcagca
     5041 ggaaggcggc aacggcgaga aagatcccgg ggatccacac cgacgagacg cggttctgcg
     5101 cgatctcgtc ggagatgaag gtatgcgaga cgtgctcgcc gcggccgtag gcgccaaggc
     5161 cgccgtagcg gccgagcagg gcgtcgaggc gggcgatcac gtcggcctcg acggcgtcgg
     5221 cggtgagcgt gatcgcaagg tcgttgaacg ccccctcgag attgaaggca ctggccagcg
     5281 ccttctcgcc catccagagc acgccgaaac gccggttgtc gggcagcagc gcgccgccgc
     5341 cgacctcgta gagaaactcc ggcgagtggc cgatgccgac gatctgcagc cgccgccagc
     5401 ggccgttgac gaccgcgccc agcgtatcgc cgggcgccag accgttggcg cgcgcgaaag
     5461 ccttgctggc gactatttcc gacggacgtt gcgggtcggg ccgccgtccg agctcgagat
     5521 agaggtcatt gagcgtcggc gccgcagtct ccgggagcga gaccagacgc ccgttggccg
     5581 gttctccgag gcccgggacg tcgagtgtca cccccatcac cacgcgaccg gcggcagcgg
     5641 caacgccagg cagactgccg atctgcggca gcagcgaccg cggcgcccgt ttcagctggg
     5701 tgaagacgtc ggcaaagcga tagtttcggt aatagtcgga ttgggcgatg cgcagcgaat
     5761 cgtaggtaca gcgcatggcg acgaatgagg cgacgccgca catcaccacc agcgcggcgg
     5821 cgatcacctg accgcgcagg tgccacaggt cgcgcagcag catgcgctgg cgcgccgcta
     5881 ccatgacagc tccgccggcg tcagccggtg gctgttgtag tcgatcccgg cgatcgtgcc
     5941 gctgctcatg cgcacgaccc ggtcggccat cgccgcgatc gcggcgttgt gggtgatgac
     6001 ggccagggtc gtgccgagtt ccgcattgac cgccgccagc gcctcgagca cgatccgccc
     6061 ggttgcgtag tcgagcgcgc cggtcggttc gtcgcagagc aggacgtctg gccgcttggc
     6121 gatagcgcgg gcgatcgcca cgcgctgctg ctcgccaccc gagagctgtg ccggaaaatg
     6181 gtcgagccgg tcgccgagcc cgacgcgctc cagtgcctcg cgcggatcca tcggctgctc
     6241 ggcgatttcg gtgaccagcg cgacgttctc gcgggccgtc aggctgggga tcaggttgta
     6301 gaactggaag acgaagccga cgtgctcccg gcggaaacgc gtcagcgccg tttcgtcggc
     6361 ggtgctcagc cggtgatcga ggtagtcgac ctcccccgaa gtcggcgcgt cgagcccgcc
     6421 gaggatgttg agcagcgtgg acttgccgct gcccgaggcg ccgagcagaa ccatcaactc
     6481 gccggcgtag agttcaaggt cgacgccgcg caacgcatgg acctcgactt cgcccatgcg
     6541 gtagaccttc gagacctggc ggacgcggaa gaccgcgttt ccctgctgcg caccggttgc
     6601 cacgacacat tccttgtcag catgcacccg ctgcggcaga aagcagcgat gcaccgatgc
     6661 tgtcgagctt acgcgttcga gtcctggctt ccttgacctc ggtcagtatc tgccgccact
     6721 gccgggcccg gcggttcggg ccggtcgcgg gtgcgtgcga gaaagagcgc gcacttcgcc
     6781 atcaattgcg ccgacaccgg tgccgtcagg aagatgaaca gcgtaatcag cagctcgtgg
     6841 acgctgaccc cctcgccacg cgacgagaag tagagcgccg aggcgatcag cacgcagccg
     6901 acccccagcg tgccggcctt ggtcgggccg tgcagccgca gcagcacgtc ggaaaagcgc
     6961 acgagcccga tcgagccgag cagcgtgaag ccggcgccga gcagcagcag gatggcgacg
     7021 aggatgtcga ttgccggcgg catcagtcga tcacctcgcc gttgagcagg tacttggtca
     7081 gcgccacggt gccgacgaag ccgagcagcg cgatcaccag cgcgccctcg aagtagagcg
     7141 cgctgtcggt ggcgatgccg ccgacgacca gcagtgccac ggtattgatg taaagcgtgt
     7201 cgagcgcgag gatacggtca acgcgcgtcg ggccgcgcgc cagtcgccag ccgcagagca
     7261 gcatggacag caccagcatg ccgaggacga tcggcaggac gctttcggtg actccgctca
     7321 tcgcagcatc tccagcaaga ggtcttcgta gcgctgccgg atctcgtcgg cgagcgccgc
     7381 cgggtcgggc gcatcgagtg cgtgcaccag cagctggtag cgactggtag ccgagtcctc
     7441 gacgatgtcg gccgagagcg ttcccggtgt cagcgtgacg atgccggcga gtgccgcgac
     7501 cgcccgcggc gaatcgaggc gcaaggccac cttgaccagt cccggcgaca gcgcgccttc
     7561 gcgaacgaag aggatcttcg cggcgaccga caggttggcg gtcacgatgt cgagcagaag
     7621 gcgcccgagc agtcggagtg ccaaccaagg cgcgtgcttc gccagcgggc gctcgcgccc
     7681 agggccgcct cccagcgaga cgaaaagcag cggtacggcc cagccgacca gcagcgccag
     7741 cagcagttgc ccggcggcca gcgtgttgtt aagtaccagc cagagcgccg ccagcgccag
     7801 cgacaggaag ggggccggga accagcgggt catggctgcc gggctccttg cggacgcggc
     7861 agcgtgccga gtacgcctgc tgccacgatt cgggttgatg ccacctgttc ggccagcgcg
     7921 ctgagatagc gttgcgcggg gccggcggcg accgtgagca gcacgaggcc gccgagcagc
     7981 agccaggcgc cccagaggtc gccgcggccg ccgcgccgta cgtgttcgtc ggcgcggtgc
     8041 ggcgtcgtct gccagaacag cagcgtcccg gcgcgcgcca gcgccagcag cgtggtcagg
     8101 ctgccgagca agacggcgac ggcgaggtgg gcgcggcccg gctgggcgaa gacttcggcg
     8161 agcagcatcg ccttgccgat gaatccggtc agtggtggca gaccggcgat ggcgacggcg
     8221 acgacgaaga acatgccgcc gagcaggccg gcgcgaccga ccgccatcgc cggttcaagc
     8281 cgatcggagg cctcgccgcg gccgcggcgg atcgcgtcgg caacgagaaa cagcgcggcg
     8341 ccggcgagcg tgctgtgcgc cgcgtagtac atcgccgcgg cgacgccgcc gggcaggccg
     8401 gcggcgatca gcagggtgcc tgccgaggcg aggatcaggc tggcggcaat gccacgcagg
     8461 tggcgtgccg ccagcgcgcc gaaggcgccg aacaagagcg tcagggcgcc gacccaggga
     8521 atcgcgccag ccaggtagat ccacggcgtc gatggctcca tcgcgccaaa gacgccgacg
     8581 aaaacgcgga tcaccgcgta gatgccgacc ttggtcagca gcgcgaagag cgccgcgacc
     8641 ggggcatcgg cagcgccata ggtggccggc agccagagcc cgagcggcag cgccgcagcc
     8701 ttgatcgaga agaccacgag cagcagcagc atcgccgcgc gcagcagcgg tacgtccgcc
     8761 tccggcagct ggctgccgat gctcgccagc tcggcaaaac tgagcgtgcc ggtgacgccg
     8821 tagatcagcc cggcagcgat caggaagagc agcgagccga ccagattgaa ggtgatgtag
     8881 tggaggctgg cgcgccgcgt ctgccgctcg cccgaataga gaagcaggcc gtaagaggcg
     8941 gcgagcagga cctcgaagca gacgaacagg ttgaacaggt cgccggtgag gaaggcgccg
     9001 gcgacgccgg cgagctggat ctgaaacagc ggatggaaat gcagtttgcg cgcgtggccg
     9061 ccggcgatcg ccgcaatcag tgttgctgcg ccgacgaagg cggtcagcag cagcatccag
     9121 gctgagagcc ggtcgaggac gaggacgatg ccaaagggcg cgcgccagtt gccgagcagc
     9181 agcgtctcgg tggtgccgtc tgcggcacga ccgaatagca ccagcgcgat gccgatgaag
     9241 gccagcgtcg cgatcagccc gaaaccgtgg cgcgcagtgg gcggtaggcg gcgcagcagg
     9301 accatcgtcg ccgcagcgac gatcggcagc accaccggag ccacgagcag gccgctcatt
     9361 gctcgtcctc cggctcggcg tcgtggcgca ggcggcgttc gccgggctcg ccatcgacgt
     9421 gatcggtctt ctcggcgacg aagtcggcga gcgcgacagc gagcagcaga gcggtcatcg
     9481 cgaagccaat gacgatagcc gtcagcgtta gcgcctgcgg cagcggatcg gtgtactggc
     9541 ccgcttcgcc accgacgagg atcgctgccg cgccgcccag gcgccccatc gagaacagga
     9601 agagattgac gccgtaggcc atcagggtca gcccgagcac cacgtcgaag ctgcgcgcgc
     9661 gcagcaccag aaagacgccg cagccgatca ggacgccgat gccggtggcg agcagtagcg
     9721 ccatcagcca ccccctccgg tgccgtgcga tccaaccgtg cgtaggcggc caagggcaac
     9781 gatcgccgcc agcgtcgcgc cgacgacgac ggcaaagacg cctaggtcga acagcgcggc
     9841 gctggccagc ggcagttccc cgatgacggg caggaccggg tggccgaagg tgctggtgag
     9901 gaaggggtag ccgaacagcg ctgcggcggc gccgctgccg ccggcgatca gcagccccca
     9961 gccgagccag ccctgccagt cggcggcgag ccgtgactcg acctggccgc ggccgcgggc
    10021 gagataaagg gtgacgacgc cgatggcgta gaccagcccg gcgatgaagc cgccgcccgg
    10081 caggttgtgg ccgcgcagga agaagtagag ggcgacgacg gcggcgagcg gcagcagcag
    10141 gcgggacacg acgcgcagca gcagcgggtg gcggaccgga tcgacagcct cggggtgggt
    10201 cagcgccgcc ggtggcggat gcaggtggcg cagcagcgca acgatggtaa gcgccgcgac
    10261 ggcgagcacg gtgatctcgc cgaaggtatc gaagccgcgg aagtcaacga ggatgacgtt
    10321 gaccacgttg gtgccaccgc cctcgggcac ggcgcgcgac aggtagaagt cggcaaggga
    10381 ctcgcgcggg aacagcccgg ctggcgtccg cgtcatcacc gccaatgcca gcagggcgat
    10441 gccgacgccg acggccagcg caatcgcgcc gtcgcgcagc agctgccggg tttcggcagg
    10501 cgcgcgcgcc ggtaccgatt cctgcggcag gtgatgcagc gcgagcatca tcaggaccac
    10561 ggtgacgacc tcgaccagca gttgcgtgag catcaggtcg ggcgcggcca gatgctcgaa
    10621 gcttagcgcg acgacgatgc cgacggcgcc gacaaaggtc agcgccgtcg cccgccgccg
    10681 gtgcagcgcg acggcgccca gtgcggcgcc gacgccgacc agccagatcg tcagctcgac
    10741 cgcatcgggc gcggtgagcg gcagcgggcc gagcgtgtac gggccgagca ggaagggcag
    10801 gccgccggcg gccaggatca ccaggaagag aagggtcagg tagcgctgca ggctgccgtt
    10861 gcggaattgg ctgatttccg cagcgaagcg cgcgaagcgg ctgagcgtcg aatcaaaagc
    10921 ctgtttgcct cgggtctgcg taatttgtgc gtgtaggcgg aacaggcgct gcaggccaaa
    10981 gtacagcgcg gcgccggcgg tcatcgccag gaagctcatc gccagcggcc agttgaagcc
    11041 gtgccagatc gccaggtcgt actcgggcat tccgccgggc agcagcggcc cggcgctgac
    11101 cgcgagaatc tcgccgacgg caagctcggg cgccaggccg accaccaggc agagtgccac
    11161 cagcaggccg accggtacct tcatccacag cggcggctcg tgcggcgtgc gcggcaggtc
    11221 gaccggctcg ccgttgaaga agacgtcgtg tacgaagcgc agcgagtagg cgatggcgaa
    11281 tcccccggcc agggtcgccg ccgccggcgt gatgtactcc agcagctgat ggccttcgag
    11341 gtagagcgtc tcggcgaaga acatctcctt ggagatgaag ccattaagca gcggcacgcc
    11401 ggccatcgcc gccgccgccg tcatcgccag cgtcgccgtg tagggcatga atcgccagag
    11461 gccgttgatg cggcgcatgt cgcgcgtgcc gcactcgtgg tcgatgatgc cggcggccat
    11521 gaacaggctg gccttgaagg tcgcgtggtt catgatgtgg aagatcgccg ccaccgctgc
    11581 cagcggtgag tcgaggccga gaagcagcgt gatcagcccg agatggctga tcgtcgaata
    11641 ggcgagcagg cccttcacgt cgtgctggaa gatggcgacg aaggcgccga gcaagagcgt
    11701 cgccatgccg accgtcgaca ccgtccagaa ccagatctcg ttgcccgcca gcgccgggta
    11761 aagccgcgcc agcaggaata cgccggcctt gaccatggtc gccgagtgca ggtaggcgga
    11821 aaccggcgtc ggcgccgcca tcgcgtgcgg cagccagaaa tggaagggga actgcgcgct
    11881 cttggtgaag gcgccgagca gcaccaagag cagcgccgcc ggaaacgccg ggtgcgcctg
    11941 caggacttcg cgcgcggcga gcacttcgtc tagcccgaag ctgccgacga tgtggccgat
    12001 caggagcacg ccagccaaca ggcagaggcc gccggcgcca gtgatcgcca gcgccatgcg
    12061 cgccccctgt cgggcatcct gccgatgctg ccaaaaaccg atcagcagaa aggaggaaag
    12121 actggtcagc tcccagaaga cgaccagcag cagcaggttg tcggcggtta ccacgccgag
    12181 cattgcgccc atgaagccga gcagcatcgc gtagaagcgc ggtgctggtt cgctggcgtc
    12241 gaggtagtag aaggcgtaga gcacgaccag cgcaccgatg ccgccgatca gagagacgaa
    12301 cagccaagcc aggccgtcga cgcggaaacc gaaggcgacc gccggcgttt ccatccaggc
    12361 gacctgccag cgcagcacgg tgccgtcgaa gatcgccggt gcgaccgcca gcgcgtaggc
    12421 gaaaaccgct gctgccgcga gcccggcgat cagcgccgcg gcggcgcgcg accgcacgct
    12481 gccggcggcg atcgcagcag caaagggcag cagggtgaca aagggcagca gtccgtcggg
    12541 cgtcatggtc gttcgggtcc ggccaggcgt cggccgctgg acagcggaac cagcgggggg
    12601 cggttgcagg gaaaaacaaa ctctcagcgc ggtgattcta tccggtcggg gggttttccg
    12661 cggttcggga tggcgaacct atccgagctt cgcggttttg acttcgcagt gccgccccgt
    12721 gttccccgcg tcgcccaatt cgccagactg tcggcagcta agccacatcg ccggtaggca
    12781 aatcgatgcc gatgcgctag cgttagacga tcggccgcaa ggccattcgc caccttggcg
    12841 cccaccatga ccattgcccg tcttctcgcc tgcctgctga tcgcgcttgc cgctgcgccg
    12901 ctaagcgcgc aggcgcagtg ccgcgccgac gccgtgtcgg cgctgccgat gccggcggca
    12961 acctggctgc gcagctggac cggcccgctg gcgagtccgg cgcgtctcga tgtcgatgcg
    13021 gccgggcggg tgctcgtcgc tgatccggcg cgtggtcgcc tggtccgccg cgccgccgat
    13081 ggctcgttga tcgagcagat cgccacgggc gggcagccga gcgcggtggc catcgacccg
    13141 gccggcgggt tctgggtcgg cgacgccggc aagggcaccg tcacccgcca cgatgtcgac
    13201 ggtcaggcgc gctttgcgct cggtgtcggc gacggcgagt tcggccagcc cggcgacatc
    13261 gcagtcgatc cggccgacgg cagcatttac gtcagcgata ccgcgcggca gcaggtcagc
    13321 gtctactcgc cgggtggcgt tcgcctgcgc agcatcggca ccgcggcacc ggccgacgac
    13381 ctgccggcgc ccggtggcca gttccgcacg ccgaccggca tcgtcatcgc cggcgacgaa
    13441 ctgctggtcg gcgaccagct caacttccgc atccaggcct tcgacaagca gagcggcgcc
    13501 ttccgctact gccttggaac ttttcgcgcc agcagcttct tctcgcccaa cagcggcccg
    13561 gcgcgcagct ttggcaccgt gcagggcctg tgggtcgacg ccctcggccg gctctacgtg
    13621 gtcgatgcct accaggggca ggttgttgtt gtcgatcgca gcaatggcgc gatcatcggc
    13681 cgcatcggca gcttcggcga cgagcccggc gcgctgcgcg ttcctgccga cgttgtgatt
    13741 gacgggaacg gcaggctctt cgtcagcaat ggtgacgctg gccgtctgga gatcttcggc
    13801 ctcgacggct ttgtcgacct cgaggccgtg gttccgggac atgtgcagat cgagccggcg
    13861 cgtatcgacc ggcgtgcgcc gccgggagcg ctggccctgg cggtcgaggc gcccggctat
    13921 cgcagcatcg atatcgaccc cgatgacgtc accgtaaacg gcgtcgcggc gcaggtcctg
    13981 gaagtcgccg atgccgatgg caacggtgtt cccgagttgc ggcttacgct gcgtactgcc
    14041 gatctgctgc cgacgctggc cgacggcgag gcggtcgcgg tttcggtgag cgccccactc
    14101 ggcacgttgc gcctggtcgc caacacctcg gttgcagtga tcggcgacct gcccggcgac
    14161 agcgatggcg acggcatcga cgacttccgc gaccgctgcc cagccagtgc agctggcgct
    14221 gtcgtcgacc ggcagggctg cgccatcgcc cagcgctgcc aatgcgacgg cagtggcagc
    14281 gacaagcgta agcacgatga cgacgatcgc caccggcatg cgcacggcgc ttacgtccgc
    14341 tgcgtcgaaa aggccgctgc ggagttacgc agcgccggcc gcatcacgca gcaggccttc
    14401 cgctcgctgg ttcgggacgc cgcgcagtcg tcctgcggga gaacgcgatg agccgccttg
    14461 ccagtttgtt cgctgctgcg ctgctggccg gcgccaccat gcaggcgctg gcgctcgatc
    14521 cgccgcacga cgccagccgc aacattcagt gcatcacctg tcacatgctg cacggctcgc
    14581 ccggcggcac gctcaaccgc gttgccggca acgccaacgt ctgcctgagc tgccacgtgc
    14641 cgaccggcca ggccgcggca cgcccgttcg ccgccgccgg gcaggcgctg ccgcccgagc
    14701 cgcgcccgac cggctggacc gccggcagcg gcatttcgca ccgctgggat tcggggccga
    14761 gcggtcacgt caagccggtg tcgggcaaca cctcgaccgg ccacatcgcc tcgctgggca
    14821 gcttcaccgg gcgcatcgaa gagacctaca cgatcaccat cgctacggca ggcacggtcg
    14881 gcaccgcgac cttcgactgg agcagcctgc gcgacggttc gggcagcgcc gtggtcaccg
    14941 ctccgagcgt cggcatcggc agcaagggcc tgtcgcttgc cttcgccaat ggcagcggca
    15001 cgccctcgtt ccgttcgggc gaccagtggc tgctgcgggc gcgcaccgac ctgcgtctgc
    15061 ccgaccagtt cgacgtcttc gagcggcagc tctggcagcg cgtcatgcgc gaggaactgc
    15121 ccgaccgcac gacgctcggc gaccccaagg tcgtctgctc gacctgccac gaccagcaca
    15181 gccagaaaca ccagcccttc gacgcggcgg cgccgatctt cctcggcgcc ggcaccggct
    15241 ggatcagtac cggcatcggt cgccatttgc agcgccagcc gaacgagaac aaccagatgt
    15301 gcaaggtctg ccactcggcg cgcaacgtcg ccagcgccgc cgccggttca cacccggtcg
    15361 gcgtggcgat tcccggcggc gatttccagt cgccggccgg cgtgccgctg tcggccgagc
    15421 tgcgcgttga atgcatgagt tgtcacgccc cgcactacac ggactcgggc ggcgccaacg
    15481 ccggcaacgg cgacggcttc ctactgcgca cccgcatcaa cgacttgtgc ttcgagtgcc
    15541 acaccaacgc tgatcgcgcc aatggcgcgc acttcaacac caccaccggc gccctatggg
    15601 gcggcagcaa ggaggccaac ggcacgcagt tcccgcggca ccaggctggg atgcgcggag
    15661 cctgtgtcaa ctgccactgg ccgcacggct ggccagaccg aaatgcgccg gcacaggatt
    15721 accctcgcct gtgggtcgag cgctacgacg tcgaccgcac gacgaagacc gacggcgccg
    15781 acggcgagct gctctgcttc gcctgccaca ccgcgccgag tggtggcgtg ccggcgacgc
    15841 cggcgacctc gaatattcag gccgagttcg cgcgcggttc ggctctggca agcgcgaccg
    15901 gcgacgtctt ccgtcacccg gtcaacgacg tcgagcagca gaacggtaac aacggcttgc
    15961 gtgtcgtcga gtgcgtcgac tgccacaacc cgcacaaggc gcgcgccaac gatcgccacg
    16021 ccggcgtttc cggtgtcaac ctggcgggta cgacgctgcc ggcaggcagc agcctgcagc
    16081 agcaagaggt ctgcttcaag tgccacggcg acacgttcaa cagcgcgcgg cccaacgcca
    16141 gcaacaagcg gctcgacttc aatacctcgg cggccaattc gggctaccac ccggtgacgc
    16201 aggccggtcg cggccagtcg gccaacctcg ctgcacagct gctcggcggg ctgacgacgg
    16261 cgtcgacgat ccgctgcagc gactgccaca actcgagcgc cttttcaggc gcggtcggca
    16321 cgattgccga ttcgcctgct gttaccgtcg gcccgcacgg ctcggaattc gcgccgatcc
    16381 tgcgcgccga tttcggccgc aactacacca gtaccggctg gaacaacgcc aacgcgacgc
    16441 tgtgtttcgg ctgtcacgac caaacgatgc tgctggcgcg ggatcgtggt tcaggtgcaa
    16501 gaacaaactt ctacggtggt ggcagggaca acctgcactg gtaccacttg gaaaacaaag
    16561 gggtcacatc aagctgcatg agctgccatt tcgacttgca cagcaaccgc acggcaagca
    16621 acacgcagta ccgcgtggtt gatggagcca cgcagaccta tctgggtacg gcaccgccac
    16681 cgaacgtgaa aacgcacctg gtgaacttcg cgcccgatgt cactggcgcc agcttcgcgt
    16741 tgccgcggtg gcagatcaac acggcgaacg gtgttcgcac atgcgatctg gcctgtcacg
    16801 gcgacggagg aaagatggat cccgtttcct acgggccgac gactggcgac gagaccagcc
    16861 acacctactg aggagtcggc gttcagtccg gtgccgtcgg ctgctcgtcc gcctcagtcg
    16921 gcgccgagca gcgcccgcgg gtgcaggttc agcgcgctgg cgccgtcgcc gaagagcagt
    16981 gtgccgtcct ggatcgtcgc cgacagccgc atcgtccgcg ccgccatcgc cgccatcgcc
    17041 tgcgactcct cggggtccaa cgctaggacc ctgagcttct tctgccggcc gagccggttt
    17101 tcgattccct gccaccacat ccgcgccgcg ttgccaccgt aggcgatgac gctgacgcgg
    17161 tctgcccggc cgcaggcacg cgtgatccgc cgctcgtcgg gcaggcccac ctctatccaa
    17221 tgcgtgattc gcccggtcag gtcgtcctcg accagtgccg gttcgtcttc gtccgacagg
    17281 ccgcggccga agcgcaggcg ctcgctggca aacagcgcga aggcgagcag gcgaatcatc
    17341 atccgctcgt cggtttccga agggtggcgg gcaatcgtca gcggatagtc ggcgtaatgg
    17401 tggcggtcga ggtctgcaag ttgcagatcg accttgaaga ccgttgattt gagagccatc
    17461 gggttccagg cagggcgacg tccggtgcgg gcggcgcagt gtagcagtcg gcgtatcgcc
    17521 gacgttcagc cgctggaatg cgattcggac agttcgtcgg cgagggcttc cggaaggcga
    17581 tatgcgcgcc aggcgccagc gagaccgagc agagacagtg ccagaatcac gcccgatgtc
    17641 gaaaggacag aaccggctgc actgagtaag ccagcgatca gcaggaccag accgaccgcg
    17701 gtattggagg cggcgacgta gtcggtgcgg cggttgccgg tggccatgtc gaccacgtag
    17761 gtcttgcggc cgattcgcgc accctgatgc gcaattgcca gacagaagga aagcgttggc
    17821 agcagccaaa ccgcttcgaa catggtcggg ttcacgcggt cgatggcaaa tacggcgatg
    17881 ccggtggcag acgcgagcac cgctcctagg ctcattgtct tgcggctgga tcggtcggca
    17941 aagcgtcccc agaacgggga actgagcagg ctggcgaggc cgctcgcgac gatgaaggcg
    18001 ccgagaagcg atgccgtcga ttcgtgcccg gtttgcgcca gagagatgta gaacggagcg
    18061 ctgagcgccg tgctcaagag caggcagcga gcgatgacaa agcggcgcag taatggatcg
    18121 gtgcttagca gccgcagagt cgccagtgcg tcgcttgaga ccgcatgccg acccgcgggc
    18181 tgatctggca gttcgtgcac gccggcgtag acggcagcgg cgagcagcca cagaacggcg
    18241 gcagcgaaaa cgatcagcgc gtagagcctg atgtcgccgg agccggtagg cgtgagcgcc
    18301 agcacaaggc cggtcgcgag ggtgacgagg ccggctgcgc tggcccccca ggcagtagtc
    18361 tggccgcgcc agcgctttgg aatcgtgcgg ccgaggacat ccttcgcggc gatcgaactg
    18421 aacgcccggg cgatgctgaa gaggcagaga aggacaacga tcaaccaccc ggcgaccgcg
    18481 ccgtcgaaaa gcagtatggc gcagccgatg ccggcaacgg caagtgcctg cgcaagggat
    18541 ccggccaccc atacccattt tcggattcgc cgccgctgca cgaatggagc gattgcgaat
    18601 tggggcagca tcgatcccga ttcgcgaatg gggacgagca gaccgatcag ataactgggg
    18661 gcgccgacgg cccccaacaa ccaggcgagc gtgattttcg gaccgctcag cacatcgccg
    18721 agctgcacca ggaaggtcgc gccgatcgcg cggaagaagt tgcccggaat tgcctcgcga
    18781 gcagattgct cgaagccagc cgccggatca ccgcgggtat cgaccaggcg cgcaaacagc
    18841 cgcgcaggga tacctctggt ttcgggcggc gccactacgc cggtcggagc agttgccgca
    18901 ggacgaaggg caggatgccg ccgtggcgca ggtaggcgac ttcgatcggc gtgtcgatgc
    18961 gcaggatcag cgtgacgcgc ttcgaggagc catcggcgcg tgtgatcacc aacgtcgcct
    19021 gttgtcgtgg cgtcggcgca ttgccgacgc cctcgattgc ataactctcc tcgccggtga
    19081 tgcccagcga atcgaccgag tcgccgtcgg cgaactgcag cggcagcacg cccatgccga
    19141 tcaggttggc gcgatggatg cgttcgaagc tctgcgcgat gaccgcgcgc acgccgagca
    19201 gctgtgtacc cttcgccgcc cagtcgcgcg agctgccggt gccgtattcc tcgccgccga
    19261 acaccaccgt cggcgtgcct tcggcgatgt aggccatcgc cgcgtcgtag atcggcatcg
    19321 tctcgccgtt cctgcgcgtc acgccgccc
//
LOCUS       DATDAA010000176         2233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_468072,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000176 DATDAA010000000
VERSION     DATDAA010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468072"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="VIS73_05880"
     CDS             complement(<1..1018)
                     /locus_tag="VIS73_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY5762716.1"
                     /translation="MTNDDLFHRLDQLNEIGAALSSERNIDRLLEKILVAAKTITRAD
                     GGTLYRVTEDGKHLRFEIVRTDSLKIAFGGASGQSISDKFRDLPLYLPDGRPNNAMIA
                     AYATIHGETVNIEDAYTADGFDFSGTRKFDTSTGYRSQSFLTVPMKNHENEVIGVLQL
                     INAQYGEPPVAGPFSTASQRLAESLASQAAIALTNRQLIDQLEALFEAFIGMINMAID
                     EKSPYTGGHCQRVPALTMLLAEAVNDTAQGPLAGFRMSDKDRYELKIAGLLHDCGKVT
                     TPEYVVDKATKLETIYNRIHEIRMRFEVKLRDCTIEYLEDCLSGQHDEHGLKSRLDEQ
                     IKQLH"
     gene            complement(1047..>2233)
                     /locus_tag="VIS73_05885"
     CDS             complement(1047..>2233)
                     /locus_tag="VIS73_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY5762717.1"
                     /translation="IEPYPGKAKTEIRLRRDGNLEPYIEIPASYRAAIVARIKIMCDL
                     DISEKRRPQDGKIKFKKFGPLDIELRVATIPTAGGVEDIVMRILAAGEPIPLDKLGLT
                     PGNFENLKRTVEKPYGLFFVCGPTGSGKTTSLHSVLKYLNTPDTKIWTAEDPVEITQK
                     GLRQVQVNKKAGLDFATVMKAFLRADPDIIMVGEMRDPETTGIGIEASLTGHLVFATL
                     HTNSAPESIIRLLDMGMDPFNFADALLGILAQRLGKRLCKDCKTAYTPAPEEVQSLIA
                     EYCEELKHTDNWKRDPQGAAAVVQGEWQKRFAKDGKFTLYRAKGCSTCGDSGYKGRVG
                     LHELLVGTDPIKRLIQEKARVSEVLAEALNSGMRTLKMDGIEKVMQGITDIKQVRAVC
                     IK"
BASE COUNT          343 a          673 c          738 g          479 t
ORIGIN      
        1 agtgaagctg cttgatctgt tcatcaagcc ttgatttgag gccatgttcg tcgtgctgac
       61 cgctcagaca atcttcgagg tactcgatcg tgcagtcgcg caacttgacc tcgaaacgca
      121 tgcgtatttc gtggatgcga ttgtagatcg tctcgagttt ggtcgccttg tcgacgacgt
      181 attccggcgt ggtcaccttg ccgcaatcgt ggagcaggcc cgcgatcttc agttcgtagc
      241 gatccttgtc gctcatgcgg aagccggcga gcgggccttg ggccgtgtcg ttcaccgcct
      301 cggcgagcag cattgtcagt gccggaacgc gctggcagtg ccctccggtg tagggcgact
      361 tttcgtcgat cgccatgttg atcatgccga tgaaggcttc gaacagggct tcgagctggt
      421 cgatgagctg ccggttggtc aacgcgatcg ccgcctggga ggcgagcgac tcggccagcc
      481 gctgcgaggc agtcgagaac gggccggcga ccggcggctc gccatactgg gcgttgatca
      541 gctgcagcac gccgatgact tcgttctcgt ggttcttcat cggcaccgtg aggaaggact
      601 gcgagcgata gccggtcgag gtgtcgaatt tgcgcgtgcc cgaaaaatcg aagccgtcgg
      661 cggtgtaggc gtcctcgatg ttgacggtct cgccatgaat cgtcgcgtag gcggcgatca
      721 tcgcgttgtt gggtcggcca tcgggcaggt agagcggcag gtcgcggaac ttgtctgaaa
      781 tcgactgccc gctcgccccg ccaaaggcga tcttcagcga atcggtgcgg acgatttcga
      841 agcgcaggtg cttgccgtcc tcggtaaccc ggtacagcgt gccgccatcg gctctggtga
      901 tggtcttggc cgcaacgagg atcttttcga gaagccggtc gatgttgcgc tcgctggaga
      961 gcgcggcgcc aatctcgttg agctggtcga gccggtggaa cagatcgtcg ttggtcatcg
     1021 cctcgtccgt agcttcgtcg ccgccgtcat ttgatgcaga ccgcccgcac ctgcttgatg
     1081 tcggtgatgc cctgcatgac cttctcgatg ccatccatct tcagcgtgcg catgcccgag
     1141 ttgagcgctt cggcgagcac ctccgagacc cgggcttttt cctggatcag gcgtttgatc
     1201 ggatcggtac cgaccagcag ttcgtgcagg ccgacccggc ccttgtagcc ggaatcgccg
     1261 caggtcgaac aacccttggc gcggtagagc gtgaatttgc cgtccttggc gaagcgcttt
     1321 tgccactcgc cctgaaccac cgccgccgca ccctgcgggt cacgcttcca gttgtcggtg
     1381 tgcttgagtt cctcgcagta ctcggcaatc aggctctgca cttcctcggg cgccggcgtg
     1441 tacgcggtct tgcagtcctt gcacagtcgc ttgccgagcc gctgcgcgag gatgccgagc
     1501 aaggcgtcgg caaagttgaa ggggtccatg cccatgtcga gcaggcggat gatcgactct
     1561 ggggcgctgt tggtgtgcag cgtggcaaac accaggtggc cggtcagcga ggcctcgatg
     1621 ccgataccgg tggtctcggg gtcgcgcatc tcgccgacca tgatgatgtc ggggtcagcg
     1681 cgcaggaacg ccttcatgac agtcgcgaag tcgagcccgg ccttcttgtt gacctgcacc
     1741 tggcgcaggc ccttctgcgt gatctcgacc ggatcctcgg cggtccagat cttggtgtcg
     1801 ggcgtgttca ggtacttgag caccgagtgc agcgaggtcg tcttgcccga gccggtcggg
     1861 ccgcagacga agaacaggcc gtagggcttc tccacggtgc gcttcaggtt ctcgaagttg
     1921 cccggtgtca ggccgagctt gtcgagcgga atcggctcgc ccgcggcgag gatccgcatg
     1981 acgatgtcct cgaccccgcc cgcggtcggg atcgtcgcca cgcgcagctc gatgtcgagc
     2041 ggaccgaact tcttgaactt gatcttgccg tcctggggcc ggcgcttctc ggagatgtcc
     2101 aggtcgcaca tgatcttgat gcgcgcgacg atcgccgcgc ggtagctcgc cgggatctcg
     2161 atgtacggct cgaggttgcc gtcgcggcgc agccggatct cggtcttcgc cttgcccggg
     2221 tagggctcga tgt
//
LOCUS       DATDAA010000177         7489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_468309,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000177 DATDAA010000000
VERSION     DATDAA010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7489
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468309"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VIS73_05890"
     CDS             <1..1209
                     /locus_tag="VIS73_05890"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5762718.1"
                     /translation="VSLRYMMAERRSEKFESDNIETNKMLGLSFQSQGQLDLALDKFM
                     KCPVDDDEIRNHLYNLALDFERKRQFNKAGAVYRYVSDYNPEFRDVKERMERTLAMEG
                     AVMLGGSGGAALDMGTMMLKGGAGIEKPKLGRYEVEKELGKGAMGVVYLGRDPKINRV
                     VAIKTMALSQEFEGDELVEVKQRFFREAETAGRLNHPNIVTIFDAGEEHDLAYIAMEF
                     LKGHDLVPYTKPGALLPLPVVATIVAQVADALDYAHRNHVVHRDVKPANIMYEAESRQ
                     VKVTDFGIARITDSSKTKTGMVLGTPSFMSPEQLAGKKIDGRSDLFSLGGMLYQLACG
                     RLPFVAESMAQLMYKIANEAPVDIRTLNKEVPECLAKIIDKALSKDIQMRYQHGAELA
                     ADLRACLPAA"
     gene            1227..2057
                     /locus_tag="VIS73_05895"
     CDS             1227..2057
                     /locus_tag="VIS73_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEY5762719.1"
                     /translation="MAPDLSHVLEIAMRTDPGVVRSHNEDAIWSNAALGIVVLADGMG
                     GYNAGEVASGMATTLIASQLAAALSEHAPQGLDVSASRELASRYLLECIGEANASVHR
                     AAVSQPQYQGMGTTIVAGLFYDNTLTLAHIGDSRAYRFRDGELVQITKDHSLLQEQID
                     SGLITPEQAKQAAHKNLVTRALGVDEVVEIDVLDHDVRAGDIYLLCSDGLNDMLGDDE
                     IALTLQSLGANLELAATQLVQMANDNGGRDNVSVILAKITGDFHSPRTWLERFSAWLK
                     "
     gene            2079..2777
                     /locus_tag="VIS73_05900"
     CDS             2079..2777
                     /locus_tag="VIS73_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY5762720.1"
                     /translation="MASKLILSMDGLVLKEIAIAKDRMTIGRKPHNDIQIDNLAISGE
                     HAVLVTIGVDSFLEDMNSTNGTVVNGQQIKKHFLQNGDVIELGKYKLKYIVEKPAYSE
                     PVSTVPADFEKTMLIRPEAAKAVSGAAKGVAPPPPAAAAAGPGELGVIQLLSGPNSGK
                     QLELAKALTTLGKPGQQVAVITRRPQGYFITHVEGPSFPVVNGNPIDTQPRALKPNDL
                     IELAGVKMEFYLKG"
     gene            2800..5019
                     /locus_tag="VIS73_05905"
     CDS             2800..5019
                     /locus_tag="VIS73_05905"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5762721.1"
                     /translation="MTKHIVRIVLGLIVTAVLIGHAARVYHLTVIAHQDAFFYDARVR
                     ATAPGGIDERIVIVDIDEKSLGELGRWPWGRDRIAGIVRELFDHYAVAVVGFDVVFAE
                     PDESSGLSVMERIGQRELRNNTAFHEALGSLRHSLDYDRVFAETLKGRPVVLGYYFSN
                     QNVAASVGQLPPPVLPAGTFAGRNVAITQWSGYGANLPPLQQAAASGGHFVPIIDFDG
                     VTRRVPLVVEHAGAYYEALSLAVVRTMLGNPPLKAGLVDSGGKNYGGLEWFDIETARG
                     ALRLPVDEFAAALVPYRGEQGSFRYVSAADVLHKRLPVEALSGKIIVVGTSAPGLLDL
                     RSTPVGSTFPGVEVHTNVIAGMLDGTIKEKPRYMIGAEVAVLVLVGAILSFLLPIFSP
                     VRGMLLSGAVLASFIAINMSLWASGLVLPMAATLWLIALLFTLNTAWGYLIEARTKRQ
                     FTDLFGQYVPPELVDEMAKAPESYTMDGRNEELTVLFSDVVGFTTLSEGLDPKDLAAL
                     MNEYLSAMTEVIRRERGTLDKYIGDAIMAFWGAPVADPEHARHALRAAMAMRAALGGL
                     GERFRQRGWPVLDIGIGINTGTMTVGDMGSRVRKAYTVMGDPVNLASRLEGLTRVYGV
                     GVVVSETVKAALPDFAFRELDRVRAKGKTEPVAIYEPLGPIDEQSKEIRDGLRLWNDV
                     LRAYRAKDWDQADVALLNVARTCGEQRLFKLYADRVSRLRQNPPPDDWDGVTTFETK"
     gene            5016..5810
                     /locus_tag="VIS73_05910"
     CDS             5016..5810
                     /locus_tag="VIS73_05910"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HEY5762722.1"
                     /translation="MTKSASHQPVHLRVLGCSGGIGGRHLRTTSLLIDHDVLIDAGTG
                     VEDLSLPELTAIRHVLLTHVHLDHIAALPLLLDSVADARPGPLTVHALPETIEALRQH
                     IFNWTIWPDFSAIPSTERPFLQFRTIAVGETLCFDHRRMTVLPAVHTVPAVGYHIDSG
                     TNSLVFTGDTWTNDALWPIVNRIDNLRTLIIETAFPDSERGLADASRHLCPQTLADEL
                     ANLKKQVDLYITHLKPGQIELTMREIEEGVASFRPQMLQHNQVFQL"
     gene            5884..>7489
                     /locus_tag="VIS73_05915"
     CDS             5884..>7489
                     /locus_tag="VIS73_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY5762723.1"
                     /translation="MNAPESGDVAARLLFLKKLQSVTNKIHSTTNVDEIMLELSQDIC
                     ELFNADRLTVYVMAEEKTSIISKVKTGLNSFRDIKLPVSDMSVAGYVAQNRKLVNIKD
                     VYDDAELKRYSPRMQFLKEVDKRTGYRSKQMLACPIEDAGTSELLGVLQLINHKIDAP
                     FPPMIVEGVQEIAKTLAVAFMQRQKQPRTRTRYDALITEAVISAADMDMAQRTARRKD
                     LGLEEVLVREFDVRPPVIGDALSKYFNVPYEPFKADRVKPIDLLRNLKRDYVEANQWV
                     PLEETAEGIVVLSPDPERLQTSRVANNVYPRSKIVYRVTTQQEFVKTLEQYFGALADL
                     GSVTDILSDMDDGDHVDAVAADEVDAAADNELVRLVNKVIIDAHQQGASDIHVEPRPG
                     KEKTVIRFRKDGTLVPYIEIPASFRNPLVARLKIMCDLDISEKRRPQDGKIKFKKYGP
                     LDIELRVATLPTAGGLEDVVMRILAGGEPIPLDQLGLSPNNRDNLLKCVEKPYGLFFV
                     CGPTGSGKTTTLHSILGHLNKPETKIW"
BASE COUNT         1472 a         2388 c         2344 g         1285 t
ORIGIN      
        1 gtatcgctac gctacatgat ggcagagcgg cgaagcgaga agttcgagtc cgacaacata
       61 gagaccaaca agatgctcgg gctttcgttc cagagccagg ggcagctcga ccttgctctg
      121 gacaagttca tgaagtgccc cgtggacgac gacgagataa gaaaccacct ctacaacctc
      181 gccctcgatt tcgagcgcaa gcggcagttc aacaaggccg gcgcggtcta ccgctacgtc
      241 tccgactaca acccggagtt ccgcgacgtc aaggagcgca tggagcggac cctggcgatg
      301 gagggggcgg tgatgctcgg cgggagcggc ggtgccgcgc tggacatggg cacgatgatg
      361 ctcaagggcg gcgccgggat cgagaagccc aagctcggcc gctacgaggt cgagaaggag
      421 ctcggcaagg gcgcgatggg cgtggtctat ctgggccgcg atccgaagat caatcgcgtc
      481 gtcgcgatca agaccatggc gctgtcgcag gagttcgaag gcgacgagct ggtcgaggtc
      541 aagcagcgtt tcttccgcga ggcggaaacg gccggtcggc tcaaccaccc gaacatcgtg
      601 acgatcttcg acgccggcga ggagcacgac ttagcctata tcgccatgga attcctgaag
      661 gggcacgatc tcgtgcccta cacgaagccc ggcgcgctgc tgccgctgcc cgtggtggcg
      721 actatcgtcg cgcaggtcgc cgacgccctt gattacgcgc atcgcaacca cgtcgtgcat
      781 cgcgacgtga agccggcgaa catcatgtat gaggccgagt cgaggcaggt caaggtcacc
      841 gatttcggta tcgcgcgtat taccgattca tcgaaaacca agaccggcat ggtgctcgga
      901 acaccctcct tcatgtcgcc cgaacaactt gccgggaaga agatcgacgg gcgctcggat
      961 ctgttctcgc tgggcggcat gctctatcag ctggcttgcg ggcgtctgcc cttcgtcgca
     1021 gaatcgatgg cccagctcat gtacaagatc gccaacgagg cgccggtcga cattcgcacg
     1081 ctcaacaaag aggtgcccga atgcctggcg aagatcatcg acaaggcact ctccaaggat
     1141 atccagatgc gttaccagca cggcgccgaa ctcgccgccg acctccgtgc ctgcctgccg
     1201 gcggcgtgat cggctgagga gagaacgtgg cgccagacct ctcgcatgtg ctcgagatcg
     1261 caatgcggac cgatccggga gtcgtgcgat cgcacaatga ggacgcgatt tggtcgaacg
     1321 cggcactcgg cattgtcgtt ctggccgacg gcatgggtgg ctacaacgcc ggtgaagtcg
     1381 ccagcggcat ggcgacgacg ctgatcgcct cgcaactcgc agcggcgctg tcggagcacg
     1441 ccccgcaggg cctcgatgta tcggcgtcgc gcgagctggc cagccgctat ctgctcgaat
     1501 gcattggcga ggccaacgct tccgtgcatc gcgccgccgt gagccaaccg cagtaccagg
     1561 gcatgggcac gacgatcgtc gccgggcttt tctacgacaa cacgctgacc ctcgcgcata
     1621 tcggcgactc gcgcgcgtat cgatttcgcg acggcgagct ggtgcagatc accaaggatc
     1681 attcgctgct gcaggagcag atcgacagcg gtttgattac gcccgaacag gctaagcagg
     1741 cggcgcacaa gaacctggtg acacgcgcgc tcggtgtcga cgaggtcgtc gaaatcgacg
     1801 tgcttgacca cgatgttcgc gccggtgaca tctacctgct gtgttccgac ggcctcaacg
     1861 acatgctcgg tgacgatgag atcgcgctga cgctgcaatc gctgggcgcc aacctcgaac
     1921 tcgcggcgac gcagttggtg cagatggcca atgacaatgg cggccgcgac aatgtctcgg
     1981 tcatactggc gaagatcacc ggcgacttcc actcgccgcg aacctggttg gagcggtttt
     2041 ccgcctggct caagtaaatt gcgaacaaag gactgaagat ggcttcgaag ctgattctct
     2101 cgatggacgg gctggtgctc aaggaaatcg cgatcgccaa ggatcgcatg acgatcggcc
     2161 gcaagccgca caacgacatc cagatcgaca acctcgccat ctccggcgag cacgccgtgc
     2221 tggtgacgat cggtgtcgac tcgttcctcg aagacatgaa cagtaccaac ggcaccgtcg
     2281 tcaacggcca gcagatcaag aagcacttcc tgcagaacgg tgacgtcatc gaactcggca
     2341 agtacaagct caagtacatc gttgagaagc ccgcgtacag cgaaccagtc agcaccgtgc
     2401 cggccgactt cgagaagacc atgctgatcc gcccggaagc tgcaaaggca gtgagcggcg
     2461 cggcgaaggg cgtcgcaccg ccgccgccag ccgctgccgc ggccggaccc ggcgaactcg
     2521 gagtgatcca gctgctttcc ggcccgaaca gcggcaagca gctcgaactg gcgaaggcgc
     2581 tgacgacgct gggcaagccc ggtcagcagg ttgccgtcat cacccggcgg ccgcagggct
     2641 atttcataac ccatgtcgag ggaccgagct ttccggtggt taacggcaac cccatcgata
     2701 cgcagccgcg ggcgctgaag ccgaacgacc tgatcgaact ggccggcgta aagatggagt
     2761 tctacctcaa gggctgatat agggccggcg ccgggagcca tgacgaaaca catcgtccgc
     2821 atcgttctcg ggctgatcgt gaccgcagtg ctgatcgggc acgcggcgcg cgtctatcac
     2881 ctgacggtga tcgctcacca ggacgccttc ttctacgacg cgagggtgcg ggcgacggcg
     2941 ccgggcggca tagacgagcg catcgtcatc gtcgatatcg acgagaagag tctgggcgaa
     3001 ctcggccgct ggccctgggg acgagaccgc atcgccggca tcgtccgcga actgttcgac
     3061 cactacgcgg tggccgtggt cggcttcgac gtggttttcg ccgagcccga cgagagttcc
     3121 ggcctctcgg tcatggagcg aatcggccag cgtgagctgc gcaacaacac ggcctttcac
     3181 gaggcgctcg gcagcttgcg gcattcgctt gactacgacc gggtctttgc cgagacgctg
     3241 aagggtcggc cggtcgtcct cggctactac ttttccaacc agaacgtcgc ggccagcgtc
     3301 ggccagctgc cgccgccggt cctgccggcc ggaactttcg ccggtcgcaa cgtcgccatc
     3361 actcagtggt cgggctacgg tgccaacctg ccgccgctgc agcaggctgc cgccagtggc
     3421 ggccacttcg tgccgatcat cgatttcgac ggcgtcaccc ggcgtgtgcc gctggtcgtt
     3481 gaacacgccg gtgcctacta tgaggcgctg tcgctcgccg tcgtccgcac gatgctcggc
     3541 aatccgccgc tcaaggcggg cctcgtcgat tccggcggga agaactacgg cggactcgag
     3601 tggttcgaca tcgaaaccgc gcgcggggcg ctacgcctgc cggtcgatga gttcgccgca
     3661 gcgctggttc cctaccgcgg cgagcagggc agcttccgct acgtctcggc ggccgacgtg
     3721 ctgcacaagc gcctgccggt cgaggcgctc tcgggcaaga tcattgtcgt cggtaccagc
     3781 gcaccaggcc tgctcgacct gcgctcgacg ccggtcggca gtacgttccc gggcgtcgag
     3841 gtgcacacca atgtgatcgc cggcatgctc gacggtacga tcaaggaaaa gccgcgctac
     3901 atgatcggcg ccgaggtggc cgtgctggtg ctggtcggag ccatcctcag tttcctgctg
     3961 ccgatcttct cgccggtgcg cggcatgctg ctgtcaggcg ccgtactggc tagcttcatc
     4021 gcgatcaaca tgagcctctg ggccagcgga ctcgtgttgc cgatggcggc aacgctgtgg
     4081 ctgatcgcgc tgctattcac gctcaatacg gcctggggct acctgatcga ggcgcggacc
     4141 aagcggcagt tcacagacct gttcgggcag tacgtgccgc ccgagctcgt cgacgagatg
     4201 gccaaggccc ccgagagcta caccatggac ggccgcaatg aagagctgac cgtgctcttc
     4261 tccgatgtcg tcggcttcac cacgctctcc gaggggcttg acccgaagga tctggctgcg
     4321 ctgatgaacg agtacctgag cgcgatgacc gaggtcatcc gccgcgagcg cggcacactc
     4381 gacaagtaca tcggcgacgc gatcatggcc ttctggggtg cgccggttgc cgaccccgag
     4441 cacgcccgcc atgcgctgcg ggcggcgatg gcgatgcgcg ccgcgctcgg ggggcttggc
     4501 gagcgtttcc gccagcgcgg ctggcccgtg ctcgacatcg gcatcggcat caataccggc
     4561 acgatgaccg tcggcgacat gggttcgcgc gtgcgcaagg cctatacggt gatgggcgac
     4621 ccggtcaatc tggcgtcgcg gctggagggg ctgacgcgtg tgtacggtgt tggcgtcgtg
     4681 gtcagcgaga cggtcaaggc cgcattgccg gatttcgcgt ttcgcgagct tgatcgtgtg
     4741 cgggccaagg gcaagaccga gccggtcgcc atttatgaac cgcttggccc gatcgacgaa
     4801 caaagcaagg agattcgtga cggacttcgc ttgtggaacg acgtgctccg ggcctatcgt
     4861 gcgaaagatt gggatcaggc agacgtcgcg ctgctcaacg ttgcgcgcac ttgtggcgag
     4921 caacgcctgt tcaagctgta cgcggatcgt gtttcccggc tgcgccagaa cccgccgccg
     4981 gacgactggg acggcgtgac gacattcgag acgaaatgac caagagcgcc tcacatcaac
     5041 cggttcacct gcgcgtcctc ggatgcagcg gaggcatcgg tgggcgccac ctgcggacca
     5101 cctcgctgct aatcgatcac gacgtcctca tcgatgccgg caccggcgtc gaggatctgt
     5161 cgctgcctga actgacggcg atccgccacg tgctgctgac ccacgttcat ctcgaccaca
     5221 ttgcggcgct gccgttgctg ctcgattcgg tcgccgatgc acggccggga ccgctgacgg
     5281 tccatgcgct gcccgaaacc atcgaggcgc tgcgccagca catcttcaac tggaccatct
     5341 ggcccgattt ctcggcgata ccgagcactg aaaggccctt tctgcaattt cgcacgatcg
     5401 cggtcggcga gacgctctgt ttcgatcatc ggcggatgac agtccttccc gcggtgcata
     5461 cggttcccgc agtcggctat cacatcgaca gcggcaccaa cagcctggtt ttcaccggcg
     5521 acacctggac caacgatgcg ctgtggccga tcgtcaatcg catcgacaac ctgcgaacgc
     5581 tgatcattga aaccgcattt cccgacagcg aacgcggact ggccgacgcg tcgcgacacc
     5641 tatgcccgca aacgctggcc gacgaactgg ccaacctgaa gaaacaggtc gacctctaca
     5701 tcacccacct gaagccaggg cagatcgagc tgaccatgcg cgagatcgag gagggcgtgg
     5761 cgagcttccg gccgcagatg ctccagcaca accaggtgtt tcagctatga ggccgtcgac
     5821 ggcggcgcgc caaccaaatt cggtctgcgc ggttcaacga accacgggca cagggggata
     5881 ccgatgaatg cacccgaatc gggcgatgtc gccgcacggc tgctcttcct caagaagctg
     5941 cagtctgtca ccaacaagat ccattcgacg accaacgtcg acgagatcat gctcgagctg
     6001 tcgcaggaca tctgtgaact gttcaacgcc gaccggctga ccgtctatgt gatggccgag
     6061 gagaagacca gcatcatctc caaggtcaag accggcctca attcattccg cgacatcaag
     6121 ctgccggttt ccgacatgag cgtcgccggc tacgttgcgc agaaccgcaa gctggtcaac
     6181 atcaaggatg tttatgacga cgccgagctg aagcgctaca gcccgcgcat gcagtttctc
     6241 aaggaagtcg acaagcgcac cggctaccgg tcgaagcaga tgctcgcctg tccgatcgag
     6301 gatgccggga ccagcgaatt gcttggcgtg ctgcagctaa ttaaccacaa gatcgatgcg
     6361 ccattccctc cgatgatcgt cgaaggcgtg caggagatcg cgaaaacgct ggcagtcgca
     6421 ttcatgcagc ggcagaagca gccgcgcaca cgaacccgct acgacgcgct gatcaccgag
     6481 gcggttattt ccgctgccga catggacatg gcgcagcgca ccgcgcggcg aaaggacctt
     6541 ggcctcgagg aggtgctcgt tcgcgagttc gatgtacgac cgccagtgat cggcgacgcg
     6601 ttgtcgaagt acttcaacgt gccctacgaa cccttcaagg ccgaccgggt caagccgatc
     6661 gacctgctgc gcaatctcaa gcgcgattac gtcgaggcca atcagtgggt gccgctcgaa
     6721 gagacagcgg agggcatcgt cgtcctgtct cccgaccccg agcgcctgca gacctcgcgt
     6781 gtcgccaaca acgtctaccc gcgaagcaag attgtctatc gggtcacgac ccagcaggag
     6841 ttcgtcaaga cgcttgagca gtatttcggg gcactggccg acctcggctc ggtaaccgac
     6901 atcctctccg acatggatga cggcgatcat gtcgacgccg tcgcggccga cgaagtcgat
     6961 gccgccgccg acaacgaact cgtgcgcctg gtcaacaagg tgatcatcga cgcccatcag
     7021 cagggggcat ccgacatcca cgtcgagccg cgccctggca aggaaaagac ggtgatccgc
     7081 ttccgcaagg acggcacgct ggttccctac atcgaaatcc ctgcgtcgtt tcgcaacccg
     7141 ctggtggcgc ggctgaagat catgtgcgat ctcgacatct ccgagaaacg ccggccgcag
     7201 gacggcaaga tcaagtttaa gaagtatggt ccgctggaca tcgagctgcg cgtcgcgacc
     7261 ttgccgaccg ccggtggcct agaagacgtg gtgatgcgaa tcctcgccgg cggcgagccg
     7321 attccgctcg accagctcgg gctgtcgccg aacaaccgcg acaacctgct taagtgcgtc
     7381 gagaagccct acgggctgtt cttcgtctgc ggcccaaccg gttcgggcaa gaccacgacg
     7441 ctgcactcga tcctcggcca tctcaacaaa cccgaaacca agatctgga
//
LOCUS       DATDAA010000178         5356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_502869,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000178 DATDAA010000000
VERSION     DATDAA010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502869"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VIS73_05920"
     CDS             <1..579
                     /locus_tag="VIS73_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5762724.1"
                     /translation="AVDGLAEVAAGLKDNPLPDSFIVEPRQSDAAGLGAFAEIARDWP
                     KVAEVHADVDWAQRLSALTGVARRALWLLSAILAVALVAVSFNTIRLQVAARQQEIEI
                     ALLVGATPAWAARPFLWLGTLEGLAAGLLAVLALYGIYAIAAPGLADLAGLFGDAAML
                     EAPSLRLATTLLIGAIAIGWAGARISVTRIGR"
     gene            complement(583..861)
                     /locus_tag="VIS73_05925"
     CDS             complement(583..861)
                     /locus_tag="VIS73_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762725.1"
                     /translation="MSGFENYRGELDEIDREIRHFAAVCGVDLANQAALQACFDENHA
                     EWADDRARASLKGLLALRLKVEVEMLEEGVQPPKLGTGEAMPADEIAT"
     gene            1018..1905
                     /gene="rapZ"
                     /locus_tag="VIS73_05930"
     CDS             1018..1905
                     /gene="rapZ"
                     /locus_tag="VIS73_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY5762726.1"
                     /translation="MRLVLISGLSGSGKSIALNVLEDAAYFCVDNIPSALLPALVDTL
                     RDEGRERVAVAVDIRSGAGIGDLPEQLRALQEADDLDVQFLFLNAKSDTLIRRYSETR
                     RRHPLVDDTRTLPEAIAEERVRLAHIAEFGHHIDTSDLSANQLRNWVREFIAIEAGTD
                     SPNRDEHGLTLLFQSFGFKHGLPLDADLVFDVRCLPNPHYHPELKPLTGCDPAVREFL
                     EAQPEVLRMREDIRRFVGDWLPAFIRDNRSYLTVAIGCTGGQHRSVYFVEWLADRFED
                     QARVIVRHRQVAASATRAR"
     gene            1902..2270
                     /locus_tag="VIS73_05935"
     CDS             1902..2270
                     /locus_tag="VIS73_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEY5762727.1"
                     /translation="MNWLRLLRAGDWLVMLAGLLAVAVLADRFWRGGPAAKAQIRANG
                     EVLIEVDLAIRKEFRIAGPLGETVIEIDRGRARVKSDPGPRQYCVLQGWLARAGDVAI
                     CAPNRVSLAVSGADIDSTAY"
     gene            2273..2821
                     /locus_tag="VIS73_05940"
     CDS             2273..2821
                     /locus_tag="VIS73_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEY5762728.1"
                     /translation="MAASNTLSQTIRLVATAEDHRIARLTAAAITLALVDAAVPMPLP
                     GIKPGLANIVTLIVLLRYGWRTAAWVSGLRVIVASLLIGQFLAPGFFLGLTGAVVSLA
                     VLAAAQHLPRRFFGPVSLSILAAFGHIGGQLLLARLWLVPHDGVFALSPVFALAALGF
                     GTLNGLIAARLLQGSPPDETTR"
     gene            2805..3434
                     /locus_tag="VIS73_05945"
     CDS             2805..3434
                     /locus_tag="VIS73_05945"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HEY5762729.1"
                     /translation="MKPPAEPATICLAWTGASGMPYGLRLLECLLTAGCRVHLLYSAA
                     AQTVARQEMDLDLPSRPAEAEAQFRERFAALPGTLSVHGREAWFSPMASGSNPPDAMV
                     VCPCTMGSLAAIAQGLADNLIERAADVVLKEGRTLVLVPRETPLSVIHLENMLRLARA
                     GAVILPPAPGFYHHPKSVDDLVDFVVARVLDRVGVPHQLMARWGEAPRA"
     gene            3493..4128
                     /locus_tag="VIS73_05950"
     CDS             3493..4128
                     /locus_tag="VIS73_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY5762730.1"
                     /translation="MKWPWQRKAGAAGEGAEVALFPLGTPLLPGGRMSLKIFETRYLD
                     LMTACLRHATPFGICMIERGSEVASPGAEPAVPHTVGTLASVEALDMREPGIMQVGVL
                     GGRRFRIDTRKVGSGGLQRGTITLLDDSPCAVPERFAKLLPLLQTVLADLGEARAPLP
                     HHFDDAGWVGHRFVDVLPVQALAKQKLLELDDPLSRLEIIEKYLQQRGLVS"
     gene            4187..4726
                     /locus_tag="VIS73_05955"
     CDS             4187..4726
                     /locus_tag="VIS73_05955"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HEY5762731.1"
                     /translation="MERAPAGPSALLESLPPVANKNAEILILGSMPGPASLAAQAYYA
                     HPQNALWPILGAIFGFDANAPYTLRLDALRCRGVALWDVLARCRREGAADATIRAVEV
                     NDFGKFFTAHRQLRRICFNGGTAARLFRRHVPPAALPRGIELLDLPSTSPAYAAMPRS
                     RKLAHWQTALCDSAPRSTG"
     gene            complement(4740..>5356)
                     /locus_tag="VIS73_05960"
     CDS             complement(4740..>5356)
                     /locus_tag="VIS73_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY5762732.1"
                     /translation="MSLALWLAFLVASILISVSPGPGAASSMSAGLSHGYRATIAVIA
                     GLQVALLLQLAVVFAGLGALLATSETAFAILRFAGAAYLVWLGIAKWRAAARPVTATV
                     ADGRGLFVQGLLVNLSNPKAIVFSAALVPQFIDPTGSQLLQFGIIAATMCAVDIVVMS
                     GYALLATSLRPWLASQRLLAMQNRVFGAVFVVAGGALALAARRA"
BASE COUNT          817 a         1948 c         1725 g          866 t
ORIGIN      
        1 gccgtcgacg gactcgccga ggtcgcggcc gggctcaaag acaacccgct gcccgacagc
       61 ttcatcgtcg agccgaggca gtccgacgcg gccgggctcg gcgccttcgc cgagatcgcg
      121 cgcgactggc cgaaggttgc cgaggtgcac gccgacgtcg attgggcgca gcggctctcg
      181 gccctgaccg gcgtcgcccg ccgggcgctc tggctgctct cggcgatcct cgcggtcgcg
      241 ctggtggcgg tgagcttcaa caccatccgc ctgcaggtcg cggcacgcca gcaggaaatc
      301 gagatcgcgc tgctggtcgg cgcgacgccg gcttgggcgg cccgcccctt tctctggctc
      361 ggcacgctcg aagggctggc cgccgggctg ctcgcggtgc tggcgctcta cggcatctac
      421 gccatcgccg cgccgggcct tgccgatctg gccggattat tcggcgacgc agcgatgctc
      481 gaggcgccct ccttgcggct ggcgactacc ttgttgatcg gcgccattgc catcggctgg
      541 gccggcgcgc gaatctcggt gacgaggatc ggccgctagc gctcaggtgg cgatctcgtc
      601 ggccggcatg gcttcgccgg tgccgagttt tggcggctgc accccctctt cgagcatttc
      661 gacttcgacc ttcaggcgca gcgcgagcag gcccttgagc gaggcccgcg cccgatcgtc
      721 agcccattcg gcgtgatttt catcgaaaca ggcctgcagc gctgcctgat tcgccagatc
      781 gacaccgcag acggcggcaa agtggcgtat ttcgcggtcg atctcgtcga gttcgccgcg
      841 atagttttcg aagccgctca tggcgcttcc tccctgaatc gcttttttcg ccattgtgcc
      901 gcgtgcgctt gaggcgcgtc ggcaaccgca gtcacgatcg tccttgcggc gggcggaaat
      961 tgccgtggcg ccggcacatc cgctatgctg ctcccgtcaa cttagcaggc cagtcacgtg
     1021 cgtctcgtcc tcatcagcgg cctctccggc tccggcaaga gcatcgcgct caatgtgctg
     1081 gaggacgccg cctacttctg cgtcgacaac attccgtcgg cgctcctgcc ggcactcgtc
     1141 gacacgctgc gcgacgaggg acgagagcgc gtcgcggtcg cggtagacat ccgcagcggc
     1201 gccggcatcg gcgatttgcc cgaacagtta cgcgccctgc aggaagccga cgacctcgac
     1261 gtccagttcc tcttcctcaa tgccaagagc gacacgctga tccgccgcta ttcggaaacg
     1321 cggcgacgcc atccgctggt cgatgacacg cgcacgctgc cggaggcaat tgccgaggag
     1381 cgcgtgcgcc tcgcccacat cgccgagttc ggccatcaca tcgacaccag cgacctctcg
     1441 gccaatcaac tgcgcaactg ggtgcgagag ttcatcgcga tcgaggccgg caccgattcg
     1501 ccgaaccgcg acgagcatgg cctgacgctg ctgttccagt ccttcggctt caagcacggc
     1561 ctgcccctcg acgccgatct ggtcttcgac gtgcgctgcc tgcccaatcc gcactaccac
     1621 cccgaactca agccgctcac cggctgcgac cccgccgttc gcgagttcct cgaagcccag
     1681 cccgaggtgc tgcgcatgcg cgaggacatc cgccgcttcg ttggcgactg gctgcccgcc
     1741 ttcatccgcg ataaccgttc ctacctgacg gtcgccatcg gctgtaccgg cggccagcat
     1801 cgctcggtgt acttcgtcga atggttggcc gatcgtttcg aggatcaggc ccgggtgatc
     1861 gtccgacatc ggcaggtcgc cgcatcggcc acccgcgccc gatgaactgg ctgcgcctgc
     1921 tgcgcgcagg cgactggctg gtaatgcttg ccggactgtt ggccgtcgcg gtgctcgccg
     1981 accgcttctg gcgcggcggc cccgcggcca aggcgcagat ccgcgccaac ggtgaggtgc
     2041 tgatcgaagt cgatctggcg attcgcaagg aattccgcat tgccggcccg ctcggcgaga
     2101 ccgtaatcga gatcgaccgg ggtcgcgccc gcgtcaagtc cgatcccggc ccgcgccagt
     2161 actgcgtgct gcagggctgg ctcgcccgcg ctggcgatgt ggcgatctgc gcaccgaacc
     2221 gggtctcgct tgcggtttca ggcgccgaca tcgactcgac ggcctactga cgatggccgc
     2281 atcaaacact ctcagccaga cgatccgcct cgtcgccacc gccgaggacc accgcatcgc
     2341 gcggctgacc gctgcggcga tcacgctggc actggtcgat gccgcggtgc cgatgccgct
     2401 gccggggatc aagccaggac tcgccaatat cgtcacgctg atcgtgctgc tccgctacgg
     2461 ctggcgcacc gcggcatggg tcagcggcct gcgggtcatc gtcgccagcc tgctgatcgg
     2521 gcaattcctc gcacccggct tctttctcgg cctcaccggc gcggtcgtca gcctcgccgt
     2581 gctggccgcc gcgcagcatc tgccgcggcg cttcttcggg ccagtatcct tgtccatcct
     2641 cgccgccttc ggccacatcg ggggccaact gctgctggcg cgactgtggc tcgttccgca
     2701 cgatggtgtc ttcgcgctgt cgcccgtgtt cgccttggcg gccctcgggt ttggtaccct
     2761 caacggactg atcgccgccc gtctgctgca aggatcgccg cccgatgaaa ccacccgctg
     2821 agcccgccac gatctgcctc gcctggaccg gcgcctcggg catgccctac ggcctgcgcc
     2881 tgctcgagtg cctgctcacc gccggctgcc gggtgcacct gctctactcg gcggcggcgc
     2941 agaccgtcgc ccgacaggag atggaccttg acctgccgtc gcgaccggcc gaggccgaag
     3001 cgcagttccg tgagcgcttc gccgccctgc ccggcacact ctcagtacac ggccgcgagg
     3061 catggttctc gccgatggcc agcggctcca atccgcccga cgcgatggtc gtctgcccgt
     3121 gcacgatggg ttcgctggcg gcgattgcgc agggacttgc cgacaacctc atcgagcgcg
     3181 ccgccgacgt cgtccttaag gaaggcagga cgctggttct ggtgccgcgc gagacgccgc
     3241 tctcggtcat ccacctcgaa aacatgctgc gcctagcgcg cgccggtgcg gtgatcctgc
     3301 caccggcgcc aggcttctat caccacccga aatcggtcga tgatctcgtc gatttcgtcg
     3361 tcgcgcgcgt gctcgaccgc gtcggcgtgc cgcaccagtt gatggcacgc tggggcgaag
     3421 cgccgcgcgc ctgagccttg ccgaagcccc ggatgaaatc ttgaactgcc ctgccgaaag
     3481 cgcggcccgc caatgaaatg gccgtggcaa cgcaaagccg gcgccgccgg cgaaggcgcc
     3541 gaggtggcgt tgttcccgct cggcacgccg ctcctgcccg gtgggcgaat gtcgctgaag
     3601 atcttcgaga cgcgctacct cgacctgatg accgcgtgcc tgcggcacgc gacgcccttt
     3661 ggcatctgca tgatcgaacg cggcagcgaa gtcgcttcgc ccggtgccga gccggccgtg
     3721 ccgcacacag tgggcacgct ggccagcgtc gaggcgctcg acatgcgcga gcccggcatc
     3781 atgcaggttg gcgtgctcgg cggccggcgc ttccgcatcg acacgcgcaa ggtcgggtcc
     3841 ggcggcctgc agcgcggcac gatcacgctg cttgacgact cgccctgcgc agttcccgag
     3901 cgcttcgcca agctgctgcc gctgctgcag accgtgctcg ccgacctcgg cgaagcccgt
     3961 gcgccgctgc cgcaccattt cgacgacgcc ggatgggtcg gccaccgctt cgtcgatgtg
     4021 ctgccggtgc aggcgctggc caagcagaag ctgctcgaac tcgacgaccc gctgtcgcgc
     4081 ctcgagatca tcgagaagta cctgcagcag cgcgggctgg tgtcctgaca gcgctgccgg
     4141 cccgatccgc gtgaacccgc gcaccgcccc ggcagcaagc tgacgcatgg aacgggcccc
     4201 cgccggcccc tcagcgctgc tcgagagcct gccgccggtg gccaacaaaa atgccgaaat
     4261 cctcatcctc ggcagcatgc cgggaccggc ctcgctggcc gcacaggctt actacgcgca
     4321 cccgcagaat gcactgtggc ccatccttgg cgccatcttc ggctttgacg cgaacgctcc
     4381 ctacacgctt cggctcgatg ccctgcgctg ccggggagtg gccctgtggg acgtgctggc
     4441 acgctgccgg cgcgagggcg ccgccgacgc gacgattcgc gcggtcgagg tcaacgactt
     4501 cggcaagttc ttcaccgcac accggcaact gcgccggatc tgcttcaacg gcggcacagc
     4561 ggcgcggctg ttccgccgcc acgtcccgcc ggcggcgctg ccccggggga tcgaactgct
     4621 cgatctgccg tcgaccagcc ccgcatacgc ggcgatgccg cgttccagga aactggcgca
     4681 ctggcagaca gcgctttgcg actcagcgcc ccggagcacc ggataaacag gtgcgacgat
     4741 caggcgcgcc gcgcggccag cgccagcgcc ccgccggcga cgacgaaaac ggcaccgaag
     4801 acgcggttct gcatcgccag cagccgttgc gacgcgagcc agggcctaag cgaagtagca
     4861 agcaaggcgt agccgctcat caccacgata tcgacggcgc acatggtcgc cgcgatgatg
     4921 ccgaactgca gcagctgcga gccagtcgga tcgatgaact gcggcaccag tgctgccgag
     4981 aagacaatcg ccttcggatt gctgagattc accagcaggc cctgcacgaa caggccacgc
     5041 ccgtcggcca cggtcgccgt cactggccgc gccgcggcgc gccacttggc gatgccgagc
     5101 cagaccagat acgccgcgcc ggcgaatcgc aggatcgcga aggccgtctc cgaggttgcg
     5161 agcaatgccc ccagcccggc gaagaccacc gccagttgca gaagcagcgc cacctgcagg
     5221 ccggcgatca ccgcgatcgt cgcgcggtaa ccgtggctca aaccggccga catcgacgat
     5281 gcggcacccg gacccggcga gaccgagatc aggatagacg cgacgagaaa ggccaaccag
     5341 agggcaagcg acatgg
//
LOCUS       DATDAA010000179         2785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_398370,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000179 DATDAA010000000
VERSION     DATDAA010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398370"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="VIS73_05965"
     CDS             complement(<1..1265)
                     /locus_tag="VIS73_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5762733.1"
                     /translation="MLGIAGVVAAVGVGYWLGAGGHAGMDGDAAAPMTDSSGRQILYY
                     RNPMGLPDTSPTPKKDSMGMDYIPVYAGDEGGDDGVVKVSAARVQTLGVKIAPVEMRT
                     LAASIRAVGRVEVNERATVDVAPKFEGWIERLFVNATGDPVKKGQPLFTVYSPELVSA
                     QKELRIAETLRAGTDGSDPTARQGAERLVEAARERLRNWDIAVTAGDPSPYLTFRSPA
                     NGIVLEKDAVEGARIMSGSKLFRIADLSTVWVIADIYEKDLERVAVGQTAAVFIDAFP
                     GRRFDARVIYLYPTLNEASRTTSVRLEIRNRDGLLRPGMFAHVELTLGDNAPRMTVPA
                     SAIIESGESQSVLIALDEGRFKPQVVTLGLRSRDYVEILEGLSEGERVVTSANFLIDA
                     ESNLKAALANFSGSSTTAKSYQATGAFEE"
     gene            complement(1283..2560)
                     /locus_tag="VIS73_05970"
     CDS             complement(1283..2560)
                     /locus_tag="VIS73_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY5762734.1"
                     /translation="MNPVSRLALAPVILLLWLSPALSAPATAPAALGADLAGLIEHAR
                     RSNPELQRRQLEAEALRERVDSAAALPDPRFQLELMDFTNAMSGGSTSLLPGEVGQTR
                     YRVTQPLPFWGKRALRGGIAEAEADVADANRRQTQIDLERRIKVAFAGHYQATHQQKI
                     LRETLDLVVGFERLVLTRYSVGLVPQQDALRAQTEMTRLKIELLDAEARHAAAAAQLL
                     ALLALEPDTALADPITLPARPQPPTHGELLRRARSSAPELQVEAGQIAAAEQARDLAY
                     RERYPDFAVGLTNNRPRTGEDSWDLMLEVQIPLQQGRRRSQEREAEHRREAALARLRA
                     RDASLAGRLGETRAAYLAELGRAELLGGTLLPQAKANLDAAVADYETGRIDFGTLIEA
                     ETQILETRLRLLDANVAAAQRLAELEMLVGAEL"
     gene            complement(2584..>2785)
                     /locus_tag="VIS73_05975"
     CDS             complement(2584..>2785)
                     /locus_tag="VIS73_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEY5762735.1"
                     /translation="VKKVDAAKGRVTIAHGPLKNLNMPPMTMVFRADSLLLANIAAGD
                     KVRFAAEDQQGKMVVTAIEKAQ"
BASE COUNT          405 a          882 c          987 g          511 t
ORIGIN      
        1 aactcctcga aggcgccggt cgcctggtag gacttcgcgg ttgtgctgct gccgctgaaa
       61 ttggccagcg ccgccttcag gttgctttcg gcatcaatca ggaaattggc cgaggtgacg
      121 acgcgttccc cctcgctgag cccttcgaga atctcgacgt agtcgcggct gcgcagaccg
      181 agtgtcacca cctgtggctt gaagcgtcct tcatcgagcg cgatcagcac cgactgggat
      241 tcgccgctct cgatgatcgc gcttgccggc accgtcatcc gcggcgcgtt gtcgcccagc
      301 gtcagttcga catgcgcgaa catgccgggg cggagcagac catcgcgatt gcggatctcg
      361 agccgtaccg aggtggtccg tgacgcctcg ttgagcgtcg gatagaggta gatgacccgc
      421 gcgtcgaaac ggcgccccgg gaaggcgtcg atgaagaccg cggccgtctg gccgaccgcg
      481 acgcgttcga ggtccttctc gtagatgtca gcgatcaccc agaccgtcga caggtcggcg
      541 atgcggaaga gcttgctgcc cgacatgatg cgcgcgcctt cgacggcatc cttctccagg
      601 acgatgccgt tagccgggga gcggaaggtc aggtagggcg acggatcgcc ggcggtcacg
      661 gcgatgtccc agttgcgcag ccgctcgcgt gccgcctcga cgagtcgctc ggcgccctgg
      721 cgggccgtcg ggtcgctgcc gtcggtgccc gcgcgcagcg tctcggcgat gcgcagttcc
      781 ttctgcgccg acaccagttc ggggctgtag acggtgaaca gcggctggcc cttttttacc
      841 gggtcgccag tggcgttgac gaagagacgc tcgatccagc cctcgaactt cggcgccaca
      901 tcgacggtcg cgcgttcgtt gacctcgacg cggccgacgg cgcggatgct ggcggcgagc
      961 gtgcgcatct cgaccggcgc gatcttgacg ccaagcgtct gcacgcgcgc ggcactgacc
     1021 ttgacgacgc cgtcgtcgcc gccttcgtcg cccgcgtaga ccgggatgta gtccatcccc
     1081 atcgaatcct tcttcggtgt cggcgaggtg tcgggcaggc ccatcgggtt gcggtagtag
     1141 aggatctggc gaccgctgga atcggtcatc ggcgccgccg cgtcgccgtc catgccggcg
     1201 tggccaccgg cgccaagcca gtagccgacg ccaacggcgg cgacgacgcc ggcaatgccg
     1261 agcagggcat gtgcggttct gttcacaatt cggctcccac gagcatttcg agttcggcaa
     1321 ggcgctgcgc tgcggcgacg ttggcgtcga ggaggcgcag gcgggtttcg agaatctgcg
     1381 tttcggcctc gatcagggtg ccgaaatcga tgcgacccgt ttcatagtcg gcgaccgcag
     1441 cgtcgaggtt ggccttggcc tgcggcagca gcgtaccgcc cagcagctca gcacggccga
     1501 gttcggcgag ataggcggca cgggtttcgc cgaggcggcc tgcgagactt gcatcgcggg
     1561 cgcggagccg tgccagggcc gcttcgcggc ggtgctcggc ttcccgttcc tggctgcggc
     1621 ggcggccctg ctgcaacggg atttgcactt cgagcatcag atcccagctg tcttcgccag
     1681 tacgtggtcg gttgttggtc aggccgacgg cgaagtcggg gtagcgctcg cggtaggcga
     1741 ggtcccgtgc ctgctcggcg gcggcgatct gtccggcttc gacctgcagt tcgggagcgc
     1801 tgctgcgggc gcggcgcagc aattcgccat gggttggagg ctgcggccgt gccggcagcg
     1861 tgatcggatc ggcaagcgca gtgtcgggtt cgagggcgag cagggcgagc aattgcgctg
     1921 ccgctgctgc gtgtcgcgct tcggcgtcga gcagttcgat cttcagcctc gtcatttcgg
     1981 tttgcgcccg aagcgcgtcc tgctgcggta ccaagccgac gctgtaccgg gtcagcacga
     2041 gtcgctcgaa gccgacgacg agatcaagtg tctcgcgcag gattttctgc tggtgggtgg
     2101 cttggtagtg gccggcgaag gcgaccttga ttcggcgctc gagatcgatc tgggtttgcc
     2161 gcctgttggc gtcggcaaca tcagcctccg cctcggcgat cccgccgcgc agcgcacgct
     2221 tgccccagaa gggcagcggc tgggtgacac ggtagcgggt ctggccgact tcgcctggga
     2281 gcaaggaagt gctgccaccc gacatcgcgt tggtgaaatc cattagctcg agctgaaaac
     2341 gcggatcggg cagcgcggcc gccgaatcga cccgctcgcg caaggcctcg gcctccagct
     2401 gccgcctctg caattcggga ttgcttcggc gtgcgtgctc gatcagtccc gcgaggtcgg
     2461 cgcccagtgc tgcgggggct gtcgccggtg cggagagcgc cggcgacagc cacaacagca
     2521 gaatgacggg cgcgagcgcc agccgcgaga cagggttcat ggcttgtcgg cgagcgtggc
     2581 tcgttactgg gctttttcga ttgcggtgac gaccatcttg ccctgttggt cctcggctgc
     2641 aaagcggacc ttgtcgccgg ccgcgatgtt ggcgagcagc aggctgtcgg cacggaacac
     2701 catcgtcatc ggcggcatgt tgaggttctt gagggggcca tgggcgatgg tgacgcggcc
     2761 cttggcggca tcgaccttct tgacg
//
LOCUS       DATDAA010000180        10065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_328470,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000180 DATDAA010000000
VERSION     DATDAA010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10065
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328470"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VIS73_05980"
     CDS             complement(<1..290)
                     /locus_tag="VIS73_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762736.1"
                     /translation="MGGGRGPAGRVALDRFLAEIEGRALRLAEFATGNRDDAHDLVQD
                     SMIKLAQNYGERDEPEWRPLFHAILQTRIRDWHRRRIVRERVLVWFRPRADD"
     gene            complement(312..806)
                     /locus_tag="VIS73_05985"
     CDS             complement(312..806)
                     /locus_tag="VIS73_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762737.1"
                     /translation="MTRTKKQLVLALLAGSLTGPLWAADDVADRATRLGERGDRIEER
                     LDDRGDRIESRMDAKGDRLEERFDAKAERARDAGKDEKAERMEAKGDRLNERFDRKGE
                     RINDRLDNRGERIDDRLDERGKRQNKRIDDRGERREHRLDRRSERVAERAGGRPEARG
                     GARR"
     gene            1055..3751
                     /locus_tag="VIS73_05990"
     CDS             1055..3751
                     /locus_tag="VIS73_05990"
                     /inference="COORDINATES: protein motif:HMM:NF015926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HEY5762738.1"
                     /translation="MTWIKNILSKDSETPETAKPASKAKAAAPVREPQPEDPPAERLA
                     AILRCRDREQALAWSLELAGDPWLGQLAIRGRFAEVRLAAVRRIVDHAVLAEVAEACR
                     NKDKGVYRHCHDQLRQQQTADDTARRAGTLADELRALLATAPLALSRLLDLEHALLAL
                     PGADEDPTVAECRPLLAEAHARLQHEALLRRDLTQCEEQAATLQAAVFAARSEWPEAT
                     TLASWEAEHARLQAACAEAPDWLAALPSTHKLAETLAVLRAALDTIAEDQRRLATALA
                     FLDSLPTDTPLDASHRDAWAALEKPANAQAAAIAESRWQTLQPAPKAPAAKTDNTEKP
                     RGDKAAKPDLGAQRKLVEAFETALAQGHLAEAATIDKTLADTVPPGALEQRLQRARSQ
                     LGQLNGWARWGTDQARDHLVTAAEQLLAAGLPVEELTKQVPALRESWKQLNGQGHPRK
                     GQWEQFDAALTAAFAPVLAKRAEDAEKHKAAKAAKRELLDVHEAFVASPAWTDIGFDQ
                     RDQWRRELLDSWRAAGHASFRDERALRKRLDALFAQVDQQAETAREAERARRGEIIAA
                     AEALAEAPDLSRAINDAKGLQRRWTPAAGAIRLARKEEQKLWKAFRAACDRVFARRDG
                     ERAAQAAEREEQAKATQAHRDAFAATLDAAEAGGSDLRTLREALQAFRNSPSGHDNAA
                     RTLEQRAQTLIERLTLDRHRARFELMKQKALLAESIEAVAGSGDTAAGSAARAEWEAL
                     TRLPTSLEAPLARRFEAATTATIASLEAGSATRAAQLLDLEIALDLPSPPALADTRRM
                     RQLERLKQHFGAASAPSADSAELLAQCLGIAASPDADAAARIDAVIGRLVQQAIDAER
                     ETRRESRPSPRHKDPTRAGRRDGRRDSGDGRGRRDRRPQPSR"
     gene            complement(3763..4332)
                     /locus_tag="VIS73_05995"
     CDS             complement(3763..4332)
                     /locus_tag="VIS73_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5762739.1"
                     /translation="MSEAETQRPEDDGDRPTLLVVDDDHTHCRLLRRAMEVRGYAVSV
                     ARSVDDALWQVQDNPPEYAVIDLNMPGGSGLMLVEKLHALDAGTCMVVLTGFASIATA
                     VEAIRRGARHYLAKPASADEIVEAFSRVEGDPSTPVPQHPITLERMEWEHIQKVLLDN
                     DGNVTAAARALKVHRRTLQRKLTRPPTAR"
     gene            complement(4329..5675)
                     /locus_tag="VIS73_06000"
     CDS             complement(4329..5675)
                     /locus_tag="VIS73_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5762740.1"
                     /translation="MRMNPPTDGSAVAAPTLENLRRLVLLRYTIIVVLLAAIAAGEWW
                     LHMHAALLPVFFCIGMLGAFNVVTQLRLRKPWPLTPVEFLAQLVADIALLGATFYFSG
                     GLANPLVSLLLLPLIVAAIVLRQSHAWGVAALSAGCYGLLVFFHRPIEHRRPPAEVYD
                     SAFHDPGLHAAAVQGAAFDQHLVGMWLTFALSAALVAAFFTRMTQSLRRREAALARAR
                     EMALRDERIVALGTLAAGAAHELGTPLGNMLMLADELEARCSADVEARSDVAELKTQI
                     RSCKRILGEMVASAGGARAEAGGPQAADAFFEELLDKWALTRPAVRLDAHWQGERPAP
                     TLVTEQTLVQALINLLNNAADAAAGKPVEVSARCRDGSLEIEIGDRGPGLTLELQERA
                     GAVFVTTKGDGGLGIGLFLANATIERAGGSVQLTARAGGGTLSEVRLPLIEKGVGS"
     gene            complement(5768..6307)
                     /locus_tag="VIS73_06005"
     CDS             complement(5768..6307)
                     /locus_tag="VIS73_06005"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY5762741.1"
                     /translation="MIARLATVVLLAALNAAALAAPPAKTQLPLVTPTAARLSTGYSD
                     GKATLSLDALRDGRAVLLNVWATWCPPCVKELPALDRLSAKLAPSVLVVALSTDDGGV
                     AQVRPFLDKLGIRHLTPLYDRDARAFQDFALRGLPTTLLIDGTGQIVARLEGIADWDS
                     PAIAEQIRQLAGSHRRAQR"
     gene            complement(6313..7293)
                     /locus_tag="VIS73_06010"
     CDS             complement(6313..7293)
                     /locus_tag="VIS73_06010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5762742.1"
                     /translation="MRRTLRKLALLATLSLATAPLLAQNIWGDAARVNGAPISNQRLE
                     RFFDEYVKNKGRNIGRMINPRVYKKLKREALDLLIEHEVLWQAAQAEGVSIPDAEIDA
                     LIEKAAAQAKSREDFMRKLEQAGFDATSYRDYVRRELAGARYLMQAAGGDVEVSDVEI
                     RAAYERNRDDFTIPETVSARHILIQVPRTASAEQKAEKRRKMEKILSELRGGADFAEL
                     ASEHSEDSSAIAGGQLGEFERGRMVPAFEQAAFALKPGQTSEVVETQFGFHLIKVEAR
                     TPEKVATLDEVREPIRRRIAAERRAGEARRITAELVKAAKVEVLVYLESD"
     gene            complement(7358..8947)
                     /locus_tag="VIS73_06015"
     CDS             complement(7358..8947)
                     /locus_tag="VIS73_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5762743.1"
                     /translation="MRRRPRAWRLPLTLAASALLALPFAASAELTPEEAAGKMVFMEG
                     KSPSGVPIQALVGPHSTPVSGEALPCASCHGPDATGRPEGAVRPSNITWPELTKSYGH
                     RTEGGRERGPYTDEHFAAAVTFGTDPSGNKLDSAMPRYVMANVDIANVLAYLKRIDTD
                     FDPGLSADRLRIGTLLPGSGRLAEAGQAMRAVMAAHLERINAQGGIFGRQLELVVTEL
                     PADRAAAEEAAEEAMRSGEVFAMVAPFAVGIEQKLFDVAEATQVPVIGPFTLSPVDHG
                     AVNRQTFYLLPGLRDQSRALADFAAKELSLTNPIAAIVHPDGEGEVQMASALEAQLAT
                     RGWDRVVQARYSAGEMPVAKLVAALQQQGVQVVFFLGGQRELAELGTEIRDAIWTPYL
                     LALGARAGAAAIRLPTTFGNRVFLAYPTLPGDISEAAATALTTDGKTAQARMGHRPAR
                     VSAYGAVRVLEEGLKRAGSDLSRAKLAASLEKLFSFESGVMPTISYGPNRRVGALGAH
                     IVTVDLTSHSFRPTGHYMRLD"
     gene            complement(8968..9663)
                     /locus_tag="VIS73_06020"
     CDS             complement(8968..9663)
                     /locus_tag="VIS73_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY5762744.1"
                     /translation="MTPRKLLLAASATLLALSSTALSAQGHEGHQNHGAPAAAEPAQK
                     MVPLPGTTAAQQPKPKRDPLAYFTDRQLIDQNGKPQRFYSDVLKGRTVVINTAYTSCS
                     DACPLITEQMVRVRAQLGDVFGKDIFFVTISSDPETDTPQAMKAFAQKNKADVPGWVW
                     LTGKKDDVDHILRKLGQWSQFAESHATHLIVWNFKTDRGRKMLPNIAPEALAAQMKLM
                     ISDDGARLPGLFN"
     gene            complement(9676..>10065)
                     /locus_tag="VIS73_06025"
     CDS             complement(9676..>10065)
                     /locus_tag="VIS73_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY5762745.1"
                     /translation="LTALLTKVQTGLGDRLGKDVALVTITVDPARDTPARLREYAAKH
                     GAKAEWAWLSGPTGTVNEVLRGFGAYAPNFEDHPPQVLVGDPRSGQWTRFFGFADPQS
                     ILAKVEELTHARHMAPAAAADMKHHHH"
BASE COUNT         1616 a         3442 c         3436 g         1571 t
ORIGIN      
        1 tcgtcgtcgg cgcgcggacg aaaccagacc agcacgcgct cgcgcacgat gcggcgccga
       61 tgccagtcgc gaatccgcgt ctgcaggatc gcgtggaaca gcggacgcca ttcgggctcg
      121 tcgcgctcgc cgtagttctg cgccagcttg atcatgctgt cctgcacgag gtcatgggcg
      181 tcgtcgcggt tgccggtggc gaactcggcg aggcgcagtg cgcgcccctc gatctccgcg
      241 aggaagcggt cgagcgccac ccgccctgcc gggccgcgac cgcctcccat gcgttgcgtc
      301 gcgccgggtg gctagcgccg tgcgccgcca cgcgcctcgg gtcgtccgcc ggcacgctcg
      361 gcgacgcgct cactgcgccg gtcaagccga tgctcgcgtc gctcaccgcg gtcgtcgatg
      421 cgcttgttct gccgcttgcc acgctcgtcg agccggtcgt cgatgcgctc gccgcgattg
      481 tcgaggcgat cattgatgcg ctcgcccttg cgatcgaaac gctcgttaag gcgatcgccc
      541 ttcgcttcca tgcgctcggc cttctcgtcc ttgccggcgt cgcgcgcgcg ttcagccttg
      601 gcgtcgaaac gctcctcgag tcgatcgccc ttggcatcca tgcgtgactc gatgcggtcg
      661 ccacgatcgt caaggcgttc ttcaatgcgg tcgccgcgct cgccgaggcg cgtcgcgcga
      721 tcggccacat cgtcggcagc ccacagcggg ccggtcagcg agccggcgag cagggccagc
      781 actagttgtt tcttggttcg ggtcatcatc gttctcccat gcagaaagtg ttgagttgaa
      841 gccgcgtcaa tctgcgatcc tcaacagggt aaacgcggca gcatttcatc ggccgacaga
      901 ccaccacgaa atttttctgc gtcacggcgc gccaatggcc gcggcgagcc atcaatgccg
      961 gcgcagcggc ctataatcgg acgctccgcg gcaatccctg cattcgtcat acgacaagca
     1021 agcttcgccg cccaacatgc ctggctgaac gacgatgacc tggatcaaga acatcctgag
     1081 caaggactcc gaaacccccg agaccgcaaa gccggccagc aaggccaagg ctgcggcgcc
     1141 ggtgcgtgaa ccgcagcccg aagatccgcc ggccgaacgg ctcgccgcga tcctgcgctg
     1201 ccgcgaccgc gagcaggcgc tggcctggag cctcgaactg gccggcgacc catggctcgg
     1261 ccagctggcg atccgcggac gctttgccga ggtacgcctc gcagcggtcc gccgcatcgt
     1321 cgatcacgca gttctcgccg aagtcgccga ggcctgccgc aacaaggaca agggcgtcta
     1381 tcgccactgt cacgaccagc tgcgccagca gcagacagcc gacgacacgg cacggcgggc
     1441 gggaacgctc gccgacgagc tgcgcgcact cttggccacc gccccgctgg cgctgtcgcg
     1501 cctgctcgat ctcgaacacg cgctgctggc gctgcccggc gccgacgagg acccgaccgt
     1561 cgccgaatgc aggccactgc tcgccgaagc gcatgcgcgc ctacagcatg aagccctgct
     1621 tcgccgcgac ctgacgcagt gcgaggaaca ggcggcgacg ttgcaggcgg cggtgttcgc
     1681 cgcgcgcagc gaatggccag aagcgacgac gctggcgagc tgggaagccg agcacgcgcg
     1741 gctgcaggcc gcctgcgcgg aggcgcccga ctggctcgcc gcgctgccgt ctacgcacaa
     1801 gctggccgag acgctggcgg tgttgcgcgc cgcgctcgac accattgccg aagatcagcg
     1861 ccgtcttgcc accgcgctgg cttttctcga ttcactcccc accgacacgc cactcgatgc
     1921 cagccatcgc gacgcctggg cggcactgga aaaacccgcc aacgcgcagg cagcggcgat
     1981 tgccgaaagc cgctggcaga cgctgcagcc cgcgccgaag gcgccggccg cgaaaaccga
     2041 caacaccgag aagccgcgcg gcgacaaggc ggcaaagccc gacctcggcg cccagcgaaa
     2101 actcgtcgaa gccttcgaaa cggcgctcgc gcaaggccat ctcgccgaag cggcgacgat
     2161 cgacaagacc ctcgccgaca cggtaccacc gggcgcgctc gagcagcggc tgcaacgggc
     2221 ccgctcgcag ctcggccagc tcaacggctg ggcgcgctgg ggcaccgacc aggcgcgcga
     2281 ccatctggtc accgccgccg agcagttgct cgcggccggt ctgccggtgg aggaactgac
     2341 caagcaggtg ccggcgctgc gcgaatcgtg gaagcagctc aacggccagg gccacccgcg
     2401 caagggacag tgggagcagt tcgacgctgc gctcaccgcc gccttcgcgc cggtgttggc
     2461 aaagcgcgcc gaggacgcag aaaaacacaa ggcggccaag gccgccaagc gcgaactgct
     2521 cgacgtgcac gaggccttcg tcgcttcccc ggcttggacc gacatcggtt tcgaccagcg
     2581 cgaccagtgg cgacgcgaat tgctcgatag ctggcgagcc gccggccacg ccagcttccg
     2641 cgatgagcgg gcgctacgca agcgcctcga cgcgctgttc gcgcaggtcg accagcaggc
     2701 agagacggcc cgcgaggccg agcgcgcccg ccgcggcgaa atcatcgctg ccgccgaggc
     2761 gctggccgag gcccccgacc tatcgcgcgc gatcaacgac gccaaagggc ttcagcgacg
     2821 gtggacgccg gccgcgggcg cgatccggct cgcacgcaag gaagagcaga aactctggaa
     2881 ggcctttcgc gccgcctgtg atcgcgtctt cgcgcgccgc gacggcgagc gcgcggcgca
     2941 ggctgccgaa cgcgaggaac aggccaaggc cacccaggca catcgcgatg cctttgcggc
     3001 gaccctagac gccgccgaag ccggcggcag cgatctgcgc acgctgcgcg aggcgttgca
     3061 ggccttccga aattcgccat cgggccacga caacgccgcc cgcaccctcg agcagcgcgc
     3121 ccagacgctg atcgagcgcc tgacgctcga tcgccaccgc gcccgcttcg agttaatgaa
     3181 gcagaaggcc ctgctcgccg aatcgatcga ggcagttgcc ggttccggcg acacggcggc
     3241 cggctcggcg gcgcgcgccg agtgggaggc gctgacgcgc ctgccgacat cgctcgaagc
     3301 gccgctcgcg cgacgtttcg aggcggcgac cacggcaacg atcgcaagct tggaagccgg
     3361 cagcgcgacc cgggccgcgc agttgctcga cctggaaatc gccctggacc tgcccagccc
     3421 gccggcgctc gccgacacgc gccgcatgcg ccagctcgaa cggctcaagc agcacttcgg
     3481 agccgcctcg gcgcccagcg ccgacagcgc cgagttgctc gctcagtgtc tcggcatcgc
     3541 ggcgtcgccc gacgccgacg cggccgcgcg catcgacgcg gtgatcggca gactggtcca
     3601 gcaggcgatt gacgccgagc gtgaaacccg gcgcgagtcg cgtccgtcgc cgcggcacaa
     3661 ggacccgact cgcgccggtc gccgcgatgg ccgtcgcgac tcaggagacg gccgaggacg
     3721 cagagaccgg cggccccagc cgtcgcgcta gccgcccacc gctcagcgtg ccgtcggcgg
     3781 tcgcgtcagc ttgcgctgca gcgtgcgccg atgaaccttg agcgcgcgcg ccgcggcggt
     3841 gacgttgccg tcgttgtcga gcaggacttt ctggatgtgc tcccactcca ttcgctcgag
     3901 cgtgatcggg tgctgcggta ccggagtcga cgggtcgccc tcgacgcggc taaaagcctc
     3961 gacgatttcg tcggcgcttg ccggcttggc gaggtaatgg cgcgccccgc ggcggatggc
     4021 ctcgaccgcc gtggcgatgc tggcgaagcc ggtgagcacg accatgcagg ttccggcgtc
     4081 cagcgcatgc agcttctcga ccagcatcag gcccgagccg ccgggcatgt tgaggtcgat
     4141 caccgcgtat tcgggcgggt tgtcctgaac ctgccacagc gcgtcgtcga cgctgcgcgc
     4201 gacgctcacg gcatagccgc gcacctccat tgcccgccgc agtaggcggc aatgggtgtg
     4261 gtcgtcgtca acgacgagca aggtcggacg atctccgtcg tcctcgggac gctgggtttc
     4321 tgcttcgctc atgagccgac tcctttttcg atcagcggca ggcggacttc gctgagtgtg
     4381 ccgccgcccg cgcgggccgt cagttgaacc gatccgccgg cgcgttctat cgtcgcgttg
     4441 gcgagaaaca aaccgatgcc gagaccaccg tcgcccttgg tcgtgacgaa aaccgcaccg
     4501 gcccgctcct gcaactccag ggtcagaccg ggaccgcggt cgccgatctc gatctcgagg
     4561 ctgccgtcgc ggcaacgggc gctgacttcg accggcttcc ccgctgctgc atcagcggcg
     4621 ttgttgagca gattgatcaa ggcctgcacg agggtctgct cggtaaccag cgtcggcgcc
     4681 ggccgctcgc cctgccagtg cgcgtccaga cggaccgccg ggcgggtcag cgcccacttg
     4741 tcgagcaact cctcgaagaa ggcatcggcg gcctgcgggc ccccggcttc ggcgcgcgcg
     4801 ccaccggccg aggcgaccat ctcgccgaga atccgcttgc agctgcggat ctgggtcttc
     4861 agctcggcga catccgagcg agcttcgacg tcggcgctgc agcgcgcctc gagttcgtcg
     4921 gcgagcatca gcatgttgcc gagcggcgta cccagttcgt gggcggcgcc tgcagcgagc
     4981 gttcccagcg cgacgattcg ttcatcgcgc aacgccattt cacgcgcgcg ggccaatgcc
     5041 gcttcgcgtc gccgcagcga ttgcgtcatg cgcgtaaaga aggcggccac tagcgccgcg
     5101 ctgagggcga aggtcagcca cataccgacc agatgctgat cgaaggcggc tccctgcacc
     5161 gctgcagcgt gcagaccggg atcgtggaaa gccgagtcat agacctcggc tggcggccga
     5221 cggtgctcga tcggccggtg aaagaacacc agcaggccat agcaaccggc gctcagcgca
     5281 gcgacgcccc aggcgtgcga ctgccgaagg acgatcgcgg cgacgatcag cggcagcagc
     5341 agcagcgaga ccagcggatt ggccaagcct cccgaaaagt agaaggtcgc tccaagcaag
     5401 gcgatgtcgg cgaccaactg ggcgaggaat tcgacggggg tcagcggcca gggcttgcgc
     5461 aggcgaagct gggttacgac attgaatgcg ccgagcatgc cgatgcagaa gaacacgggg
     5521 agcaacgccg cgtgcatgtg cagccaccat tcgcccgcgg cgatcgccgc cagcagcacg
     5581 acgatgatgg tgtagcgcaa cagcaccagc cggcgcaggt tttccagggt tggtgcagcg
     5641 acggcagatc cgtcggtggg aggattcatg cgcatggggc gagtattggc gaaggcaaga
     5701 cgggcgacaa ggcgtggtcg cgacgccacg cgcggcagga tgtcgcaggg cgctgcctga
     5761 gcggcagcta gcgctgcgcg cggcggtggc tgcccgcgag ctggcggatc tgctcagcga
     5821 tcgccggcga atcccagtcg gcgatgcctt cgaggcgggc gacgatctgc ccggtgccat
     5881 cgatcaacag ggtcgtcggc aggccacgca aggcgaaatc ctgaaaggcc cgcgcgtcac
     5941 gatcgtacag cggcgtcagg tggcggatgc cgagcttgtc gaggaatggc cggacctgcg
     6001 caacgccgcc gtcgtcggta ctcaaggcga ccaccagcac cgagggcgcg agtttggcgg
     6061 aaagccggtc gagtgccggc aactccttga cgcagggtgg gcaccaggtt gcccagacgt
     6121 tgagtaacac ggcgcgtccg tcgcgcaggg cgtcgaggct aagcgtcgcc ttgccgtctg
     6181 aatagccggt gctcaggcgg gccgccgtcg gcgttaccaa gggcagctgc gtcttggctg
     6241 gtggcgccgc cagcgccgca gcgtttagcg cggcaagcag gacgacggtg gcgagacggg
     6301 cgatcattgt ggtcaatccg attcgaggta gaccaagacc tcgaccttgg ccgccttgac
     6361 cagttccgcg gtgatccgcc gggcttcgcc ggctcggcgt tcggccgcga tgcgtcgccg
     6421 aatcggctcg cgcacctcgt cgagggtcgc gaccttctcc ggcgtccgtg cttcgacctt
     6481 gatcaggtgg aagccgaact gcgtttcgac gacctcggag gtctgccctg gcttcagcgc
     6541 aaatgccgcc tgctcgaagg ccgggaccat gcggccgcgc tcgaattcgc cgagctggcc
     6601 gccggcgatg gccgacgagt cctcggaatg ctcgctggcg agttcggcaa aatcggcgcc
     6661 gccgcgcaac tcgctcagga tcttttccat cttgcgacgt ttctcggcct tctgttcggc
     6721 gctcgcagtg cgcggaacct ggatgaggat gtggcgggcg ctgacggttt cggggatcgt
     6781 gaaatcgtcg cggttgcgtt cgtatgccgc ccggatctcg acatcgctga cctcgacgtc
     6841 gccgccggcg gcctgcatca ggtaacgggc gcctgccagt tcgcgccgca cgtagtcgcg
     6901 gtagctggtc gcgtcgaaac cggcctgctc gagcttgcgc atgaaatcct cgcgcgactt
     6961 ggcctgcgcg gccgccttct caatcagcgc gtcgatctct gcgtcgggaa tcgacacgcc
     7021 ctcggcttga gccgcctgcc agagcacctc gtgttcgatc agcaggtcga gcgcttcgcg
     7081 tttgagtttc ttgtagacgc gcgggttgat catgcggccg atgttgcggc ccttgttctt
     7141 gacgtactcg tcgaagaaac gctcgagtcg ctgattgctg atcggcgcgc cgttgacgcg
     7201 ggcggcatcg ccccaaatgt tttgcgccag cagcggcgca gtcgcaagcg acagcgtcgc
     7261 cagcagcgca agcttacgca gagttcgtct cattcgatcc tctccccggc ctggcggcgc
     7321 cgttggcgcc atcaggcctg cggcttcttc gcctcaatca atcgaggcgc atgtaatgac
     7381 cagtaggcct aaaactgtgg ctggtcaggt ctaccgtaac gatgtgggcg ccgagtgcgc
     7441 cgacacgccg gttggggccg tagctgatcg tcggcatcac ccccgactcg aaactgaaca
     7501 gcttctccag cgacgcggcg agcttggcac ggctgagatc actgccggcg cgcttgagcc
     7561 cctcctcgag gacgcgcacg gcgccgtacg cagagacccg ggccggacgg tggcccatgc
     7621 gcgcctgggc ggtcttgccg tcggtggtca gcgccgtcgc agcggcttcg ctgatgtcgc
     7681 cgggcagcgt cggataggcg agaaacaccc ggttgccgaa ggtggtcggc aggcggatcg
     7741 ccgcggcacc cgcgcgcgcg cccaacgcaa gcaggtacgg cgtccagatc gcgtcgcgaa
     7801 tctcggtacc gagttccgcc agttcgcgct ggccgccgag aaagaagacg acctggaccc
     7861 cttgctgctg cagtgcggcc accagcttgg caaccggcat ctcgcccgcc gagtagcggg
     7921 cctggacgac gcgatcccag ccgcgcgtgg ccagttgcgc ttcgagggcg gacgccatct
     7981 gtacttcgcc ctccccgtct ggatggacga tcgcggcgat cgggttggtc agcgacaatt
     8041 ccttagcggc gaaatcggct agcgcgcgac tctggtcgcg cagccccggc agcaggtaga
     8101 aggtctgccg gttgaccgcg ccgtgatcga ccggcgacag cgtgaacggt ccgatgacgg
     8161 ggacctgcgt cgcctcggcg acatcgaaca gcttctgctc gatgccgacg gcaaaggggg
     8221 cgaccatcgc gaacacctcg ccgctgcgca tcgcctcctc ggcggcctct tcggcggcgg
     8281 cgcgatcggc gggcagttcg gtgaccacga gctcgagttg acggccgaag atgccgccct
     8341 gggcgttgat ccgctcgagg tgcgcggcca tcacggcgcg catcgcctgg cccgcctcgg
     8401 caagccgtcc gctgccgggc agcagcgtgc cgatgcgcag ccggtcggcg ctcaggccgg
     8461 ggtcgaagtc ggtatcgatc cgcttcaggt aggcgagcac gttggcgatg tccacgttgg
     8521 ccatgacgta gcgcggcatc gccgagtcga gcttgttgcc cgaagggtcg gtaccgaagg
     8581 tcaccgcagc ggcaaaatgc tcgtcggtgt aggggcctcg ctcacggcct ccttcggtgc
     8641 ggtggccgta ggacttggtc aactcgggcc aggtgatgtt cgagggccgg acggcgcctt
     8701 cgggtcgccc ggtggcgtcg ggcccgtggc aactggcaca gggcagtgcc tcgccagaca
     8761 ccggcgtcga atgcggcccg accagtgcct ggatcggcac gcccgatggg ctcttgcctt
     8821 ccatgaagac catcttgcct gcggcctctt cgggagtcag ctcggcactt gcggcgaacg
     8881 gcagtgcgag cagtgcgctc gccgccagcg tcagcggcag gcgccacgcc cgcggtcgcc
     8941 tgcgcaacgc ccgttgcctg cgatcgctca attgaacagc ccgggaaggc gcgcgccgtc
     9001 gtcggagatc atcagcttca tctgcgcagc gagcgcttcg ggggcgatgt tcggcagcat
     9061 cttgcggccg cgatcggtct tgaagttcca gacgatcagg tgcgtcgcgt gcgattcggc
     9121 aaactgcgac cattgtccga gcttccgcag gatgtggtcg acgtcgtcct tcttgccggt
     9181 cagccagacc caaccgggta cgtcggcctt gtttttctgc gcgaaggcct tcatagcctg
     9241 cggcgtatcg gtctcggggt cgctgctgat ggtgacgaag aagatgtcct tgccgaagac
     9301 atcgccaagc tgggcgcgga cgcggaccat ctgctcggtg atcagcgggc aggcatcgct
     9361 gcaactggtg taggcggtgt tgatgaccac ggtccggccc ttgagcacgt cgctgtagaa
     9421 gcgctgcggc ttgccgttct ggtcgatgag ctggcggtca gtgaaatagg cgagcgggtc
     9481 gcgcttgggt ttcggttgct gcgcagcagt ggtcccgggc agcggaacca tcttttgcgc
     9541 gggttcggcc gcggctggcg cgccatggtt ctggtggcct tcatggccct gggccgatag
     9601 ggcggtcgac gacaaggcca gcagggtagc gctcgcggcc agcagcagtt tacggggcgt
     9661 catcacgctc tcctctcagt gatggtgatg cttcatgtcg gccgcagcag ccggggccat
     9721 gtgccgtgca tgggtcagct cttcgacctt ggccaggatc gactgcgggt cggcgaagcc
     9781 gaagaagcgc gtccactgtc cgctgcgggg atcaccgacc agcacctgcg gcggatgatc
     9841 ctcgaagttc ggcgcgtagg cgccgaagcc gcggagcacc tcattgaccg taccggtcgg
     9901 gccggacaac caggcccact cggccttggc gccatgtttg gcggcgtact cgcgcagtcg
     9961 tgccggcgtg tcgcgggccg gatcgaccgt aatcgtcacc aacgccacat ccttgccgag
    10021 gcgatcgcca agcccggtct gcaccttggt cagcagggcc gtcag
//
LOCUS       DATDAA010000181         9214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_153495,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000181 DATDAA010000000
VERSION     DATDAA010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9214
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153495"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..405)
                     /locus_tag="VIS73_06030"
     CDS             complement(103..405)
                     /locus_tag="VIS73_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762746.1"
                     /translation="MEFASTVVIDPDHPALPGHFPGHPIVPGVVLLDLALLAISRALA
                     LPKAPARLTQAKFLAPVGPGAELRITHSAAIAGNVRFDIHCGETRVATGSFALETA"
     gene            complement(405..1742)
                     /locus_tag="VIS73_06035"
     CDS             complement(405..1742)
                     /locus_tag="VIS73_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5762747.1"
                     /translation="MAATRLIAHDHPDAIVARRGSEAITAAQFLADVRRLAGYLPESG
                     HVLNVCTDRYRFAVGIYAALLRKTVSLLPPAHTPEMVERLREFAPDVVCLADGQVDIA
                     LPRIDCPADAAPVAGPFAVPEIDDEQVVAHVFTSGSTGLPQRHTKTWERLVRNVRGEA
                     RRLGLVDADRRASILGTVPPQHMYGFETTVMMPLQSSSAFDAGRPFYPADITAALAAL
                     PRPRLLVSTPFHLRTLLDAGLACPPADLVVSATAPLSAGLAAQAEAAFGCALLEIYGM
                     TETGQLATRRTTAGDTWQAFDGIELSTDSGPVIAAGGHIETPVALGDLVELVDATHFR
                     LLGRGADMVNIAGKRTSLGYLNHQLLAIDGVADGVFVLPDHEAADGVTRLAAIVVAPG
                     RTRQQILGALRTRIDPVFLPRPLVLVDALPRNATGKLPRETLLAMLPGHAEAG"
     gene            complement(1762..2433)
                     /locus_tag="VIS73_06040"
     CDS             complement(1762..2433)
                     /locus_tag="VIS73_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762748.1"
                     /translation="MTLPPPARFRITLAIVIGTVYFVLAHLTATIPGYEWLGVIVGVG
                     ALYALTLMMAWTSALRLPLVAIGAVAAFFIAHYWQILEQNFTWFYLFEHAGSNALLAG
                     VFGATLFERQVPLCTRLARMVHGDDLPQLVDRYTRRITIVWTGFFALNATTSLLLYLL
                     TPIGVWSTYANLLTAPLVALMFAGEYAVRVRVLPPSHHSTIAATITAFWVRQYGATRT
                     PNASH"
     gene            complement(2430..2708)
                     /locus_tag="VIS73_06045"
     CDS             complement(2430..2708)
                     /locus_tag="VIS73_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY5762749.1"
                     /translation="MTHDAALQQEVAELIVSALNLEITPADISADAVLYGDGNGDGLG
                     LDSIDILEVALVVSKRFGFQLRADNEDNVRIFSSLTSLTEHVAAHRTK"
     gene            complement(2782..4038)
                     /locus_tag="VIS73_06050"
     CDS             complement(2782..4038)
                     /locus_tag="VIS73_06050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY5762750.1"
                     /translation="MNARRVAVTGIGMVSPFGGDSSDFFARMLAGESAIRLLERDEKP
                     RPVSVPAVRCEGFDAAAAVGRGLAHTMDRFSQLGTAAALDAWQDAGLPREAEAHRTNW
                     GVSWSTALGGILTFETGYRDLYLTGRERVSPLSVVLGMNNAAASHIAIQLGLADACHT
                     YSVACASSAIALGEALLRIRSGQSQLIVAGGSDAGTLAYSVIKAWEALRVLAPGDRDS
                     APSACRPFHTERAGLVLGEGAGALILEDWEHATARGARIYAELAGYGSTCDHSHLVKP
                     EADGQIRAMRGALADARLDPSAIGYVNAHGTATREGDPTEIEAIRAVFGAGATDLAVS
                     ATKSMHGHMLGATGALEAIITVLALHGRRVPPTANLDTATIDPACTGVRHVVGGGADL
                     PRLAAAISNSFAFGGSNTVLAFRRAA"
     gene            complement(4035..4274)
                     /locus_tag="VIS73_06055"
     CDS             complement(4035..4274)
                     /locus_tag="VIS73_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY5762751.1"
                     /translation="MDTLTELRQMLQERLGTDPATVLPESSLETLGVDSLMLLDLMFE
                     FEEKLSIQMPSDLPRPDTVKDLLDSFAELQKTAKS"
     gene            complement(4300..4374)
                     /locus_tag="VIS73_06060"
     tRNA            complement(4300..4374)
                     /locus_tag="VIS73_06060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4339..4341),aa:Arg,seq:cct)
     gene            complement(4450..5361)
                     /locus_tag="VIS73_06065"
     CDS             complement(4450..5361)
                     /locus_tag="VIS73_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762752.1"
                     /translation="MKLDLQRPLDQFVASGGQLALLAFGFVAHARVDASPVLWTVVLA
                     LIAALSLYGWILALRRRRIIVDTPTAKVATAPQGFVELVGRGRLFDGNPVASPLTGLP
                     CLWYRYRTEQKSADNKWEVVQERQSDDSIILADDSGEAVIDPDRAEVITRHRERWHDG
                     DYRHTEWKLIAGDPLYVLGELKTIGGHSLDLDANDDTAQLLAEWKRDREALHERFDLD
                     ANGQIDQREWSLARSAARREVARAHREARDRADAHIVRAPADKRPFLIANIDPDDLVR
                     RFGWWSLAHATGFVASLAAIPWMLARL"
     gene            complement(5375..5950)
                     /locus_tag="VIS73_06070"
     CDS             complement(5375..5950)
                     /locus_tag="VIS73_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY5762753.1"
                     /translation="MSISTMVFLALLVVTLVYGVVVYNGFVSLRNAVEKAWANIDVML
                     KQRHDELPKLVEACRQYKQFEQETLQRVTEARTRVQSARERHDIPALGAAEGMLRAGL
                     GQIFAVMEAYPELKANEQFMQLQSRISGLENAIADRRELYNESVNINNVRVDTFPDLI
                     VARLFGFERQALLRFSSTETADVDLRKLFTE"
     gene            complement(6006..6860)
                     /locus_tag="VIS73_06075"
     CDS             complement(6006..6860)
                     /locus_tag="VIS73_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY5762754.1"
                     /translation="MIDAALASVDDYVAYLNGAEIPALRVTQRGLDSALQRIDRVTRH
                     DLSAVLLRDPLMTVKVLAYIQPMHGKSLRGDISTVGSAIMMLGIEPFFRRFAELPLVE
                     QRLADQPAVLLCVLKTIQRAQRAAEFARDWAIWRHDLNAEEVTIAALLHDIAELLLWT
                     FAPKLMLEIHRLQQANSQLRSVVAQKAVLGITTQELQLALCQVWQLPELLRLLMDDTK
                     ADHPRVRNVSLAVDLARHLAQGGWENAAIADDIKAIGTLLNLNEAATRERLGMPPEAA
                     ETDATTAP"
     gene            complement(6880..7518)
                     /locus_tag="VIS73_06080"
     CDS             complement(6880..7518)
                     /locus_tag="VIS73_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY5762755.1"
                     /translation="MKLTSDSFGNGAAIPGEFAFCIPDERSHACLGGNRNPHLAWDDV
                     PPGTQSFVVICKDPDVPSVGDDVNQEGRTIPASLPRVDFIHWVLVDLPASRREIAAGE
                     FSKEVVARGKPGPNAADGARQGINDYTGWFASDPDMRGDYFGYDGPCPPWNDSIRHHY
                     QFTLYALDTAKLIVHGAFTAKDVHAAMAGHILAEASISGTYSLNPALAEPGR"
     gene            complement(7538..9076)
                     /gene="murJ"
                     /locus_tag="VIS73_06085"
     CDS             complement(7538..9076)
                     /gene="murJ"
                     /locus_tag="VIS73_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY5762756.1"
                     /translation="MNLLRALATVSSMTMLSRILGFVRDFVIARTFGAGVATDAFFVA
                     FKLPNLLRRMFAEGAFSQAFVPILGEYKNRQGDDAAHALVNRVAGTLGLCVLLLSIIG
                     VLAAPWLVYLSAPGFAADADKFALTVSLTRLTFPYILFMSLVALAGGVLNTWGRFAIP
                     AFTPVLLNLSFIALALLLAPRLAEPVMALGWAVILGGVAQLSLQLPALAKLGMLPRPQ
                     LAWRDPGVRRVMRLMAPALLGVSVTQISLLINTIFASFLQTGSVSWLYYADRLMEFPV
                     GLLGAALGTILLPTLSRLSAEERHDEYSQTLDWGLRLCLLLALPAALALAILSAPLVA
                     TLFYHGAFTATDVLQTQRALIAYTVGLTGLLLVKVLAPGFYARQNIRTPVKIALASLA
                     VTQVLNLALVVPLQHAGLALAIGLAACFNAGFLYRGLRRHGIYQPRPGWLAFIGRLVA
                     GLAVMGAALWFFAGNAESWLHYSPIERLWRLGALVCGGAAVYFVALRVVGIRLADFRR
                     RADI"
BASE COUNT         1542 a         2856 c         3377 g         1439 t
ORIGIN      
        1 cacaacgcag cgagaggagg gtcatcacgc gcagccagaa gcggttgctg cgctcggcct
       61 gatggcgcca ggcagcggct tcgggcgcgg cgttttcggc gttcatgcgg tttccagcgc
      121 gaagctgccg gtggcgacgc gggtttcgcc gcagtgaatg tcgaagcgca cgttgccggc
      181 gatcgctgcc gagtgcgtga tgcgcagttc ggcaccgggg ccgaccgggg cgaggaactt
      241 ggcctgcgtc agccgtgccg gcgccttcgg cagcgccagc gcgcggctga tcgccagcaa
      301 ggccagatcg agcagcacga cgccgggaac gatcgggtgg cccggaaaat ggccgggcag
      361 tgccggatgg tcgggatcga tgacgaccgt gctggcaaac tccatcaacc ggcctccgcg
      421 tggcctggca gcatcgccag cagcgtctcg cgcggcagct tgccggtggc gttgcgcggc
      481 agtgcgtcga cgagcaccag cggccgcggc aggaagaccg ggtcgatccg cgtgcgcagc
      541 gcgccgagga tttgctgccg cgtgcgtccc ggcgcgacga cgatcgccgc cagtcgcgtg
      601 acgccatcgg ccgcctcgtg gtcgggcagg acgaagacgc cgtcggcgac gccgtcgatg
      661 gccagcagct ggtggttgag gtagccgagc gaggtgcgct tgccggcgat gttcaccatg
      721 tcggcgccgc ggccgagcag gcggaagtgg gtcgcatcga ccagctcgac gaggtcgccg
      781 agcgctaccg gcgtctcgat gtggccgccg gcggcgatta ccgggccgct gtcggtgctg
      841 agttcgatgc cgtcgaaggc ctgccaggtg tcgccggcgg tggttcgtcg cgtcgccagc
      901 tggccggtct cggtcattcc gtagatttcc agcaaggcgc agccgaaggc cgcttcggcc
      961 tgcgccgcca gaccggccga gagcggcgcc gtcgccgaga caacgaggtc ggcgggcggg
     1021 caggcgaggc cggcgtcgag cagcgtgcgc agatggaacg gcgtgctgac cagcaggcgc
     1081 gggcgcggca gcgcggcgag cgcggcggtg atgtcggcgg ggtagaaggg gcggccggcg
     1141 tcaaaggcgc tcgacgactg cagcggcatc atcaccgtcg tttcgaagcc gtacatgtgc
     1201 tgcggaggga cggtgccgag gatgctggcg cgccggtccg catcgacgag cccaaggcgg
     1261 cgggcttcgc cgcggacgtt gcggaccagg cgctcccagg tcttggtgtg gcgctgcggc
     1321 agcccggtcg accccgaggt aaacacatgc gcaacgactt gttcgtcgtc gatctcggga
     1381 actgcaaacg ggccggcaac gggcgcggcg tcagcagggc agtcgatgcg tggcagcgcg
     1441 atgtcgactt ggccgtcggc gaggcagacg acatcgggcg caaattcacg caggcgctcg
     1501 accatctccg gcgtgtgcgc cggcggcagc aggctgacgg tcttgcgcag cagcgccgcg
     1561 tagatgccga cggcgaaacg gtagcgatca gtgcagacgt tgagcacgtg gcccgactcg
     1621 ggcaggtacc cggccaggcg gcggacgtcg gcgaggaact gtgcggccgt gatcgcctcg
     1681 ctgccgcggc gggcgacgat ggcgtcggga tggtcgtggg cgatcagccg agtcgcggcc
     1741 atggcggcgg tcctgcggga ctcagtgtga cgcgttcggc gtgcgcgtcg ccccgtactg
     1801 gcggacccag aacgcggtga tcgtcgcggc gatcgtcgaa tggtgcgagg gtggcagcac
     1861 acgaacgcgg actgcgtact cgccggcgaa catcagcgcg accagcggcg ccgtcagcag
     1921 gttggcgtag gtcgaccaga ctccgatcgg cgtcagcagg tagagcaaca gcgaggtggt
     1981 ggcgttcagc gcgaagaagc cagtccagac gatggtgatc cgccgcgtgt agcggtcaac
     2041 gagctgcggc aggtcgtcac cgtggaccat gcgcgcgagc cgcgtgcaca gcggcacctg
     2101 gcgctcgaac agcgtggcgc cgaagacgcc ggcgagcagt gcgttgctgc ctgcatgctc
     2161 gaacagataa aaccaggtga aattctgttc gagtatctgc cagtaatggg cgatgaaaaa
     2221 cgccgcaaca gcgccgattg ccacgagtgg caggcgcagc gccgaggtcc aggccatcat
     2281 cagggtcagc gcgtagagcg cgccgacgcc gacgatcacg ccgagccact cgtatccggg
     2341 aatcgtcgcc gtcagatggg cgaggacgaa atagaccgtg ccaatgacga tggccagcgt
     2401 gatccggaaa cgggcgggcg gcggcagcgt cacttggtgc ggtgcgcggc gacgtgctcg
     2461 gtcaggctgg tgaggctgga gaagatcctg acgttgtctt cgttgtcggc gcggagctgg
     2521 aagccgaaac gcttggaaac gaccagcgcg acttcgagga tgtcgatcga gtcgaggccc
     2581 agtccgtcgc cgttgccatc gccgtagagc accgcgtcgg cgctgatgtc ggcgggggtg
     2641 atttccagat tgagggccga aacgatcagt tcggcgactt cctgctgcag cgcggcgtcg
     2701 tgtgtcatgc ggacctaagg ggttgaaagg cggtgggttt gaaggacggg cgattatatc
     2761 gcgcggccca cccgccggcc gtcaggcggc gcggcggaag gcgagcacgg tattcgagcc
     2821 gccgaaggcg aaggagttgc tgatcgccgc ggctagcctc ggcagatcgg cgccgccgcc
     2881 gaccacgtga cgaaccccgg tacaggcggg gtcgatcgtt gccgtgtcga ggttggccgt
     2941 cggcggtacc cggcggccgt gcagggcgag cacggtgatg attgcctcga gcgccccggt
     3001 ggcgccgagc atgtggccgt gcatcgactt ggtggcgctg actgccagat cggtcgcgcc
     3061 ggcgccgaag acggcgcgga ttgcctcaat ttcggtcgga tcgccctcgc gggtggcggt
     3121 gccgtgggcg ttgacgtagc cgatcgccga cggatcgagc cgggcatcgg cgagggcgcc
     3181 gcgcatggcg cgaatctggc cgtcggcttc gggtttgact agatggctgt ggtcgcacgt
     3241 gctgccgtag ccggccagtt cggcgtaaat ccgcgcgccg cgcgccgtcg cgtgttccca
     3301 gtcttcgagg atgagcgcgc cggcaccttc gccgaggacc aggccggccc gctcggtatg
     3361 aaacgggcgg caggccgacg gcgcgctgtc gcggtcgccc ggcgcgagca cgcgcagcgc
     3421 ttcccaggcc ttgatcaccg aatacgccag cgtaccggcg tcggagccgc cagcgacgat
     3481 cagctgcgac tggccgctgc ggatccgcaa caaggcttcg ccgagcgcga tcgccgacga
     3541 ggcgcaggcg accgagtagg tgtggcaggc atcggcgagg ccgagctgga tggcaatgtg
     3601 cgaggccgcg gcgttgttca tgccgagcac caccgagagc ggcgagacgc gctcgcggcc
     3661 ggtcaggtag aggtcgcgat agccggtctc gaaggtcagg atgccgccca gcgccgtgct
     3721 ccaggaaacg ccccagttgg tccgatgcgc ctctgcttcg cgtggcaggc cggcgtcctg
     3781 ccaggcatcg agcgctgcgg cggtgcccag ctggctgaag cggtccatcg tgtgggcgag
     3841 tccgcgaccg actgcggcag cggcatcgaa gccttcgcag cgcaccgccg gcaccgatac
     3901 cgggcgaggc ttctcgtcgc gctcgagcag tcggatcgcc gattcgccgg cgagcatgcg
     3961 cgcgaagaag tcggaactgt cgcccccgaa ggggctgacc atgccgatgc cggtgacggc
     4021 aacgcggcgg gcgttcacga cttcgcggtc ttctgcaact cggcgaagga gtcgagcagg
     4081 tccttgaccg tatcgggccg tggcaggtcg ctgggcatct ggatcgacag tttctcctcg
     4141 aactcgaaca tcaggtcgag cagcatcagc gagtcgacgc cgagcgtctc aagagacgat
     4201 tcgggcagca cggtggccgg gtcggtgccg aggcgctcct gcagcatctg gcgaagttcg
     4261 gtcagggtat ccatggtctt gtcatttctc cgggggcgct ggcgtcccca gcaggaatcg
     4321 aacctacaac tgctccttag gagggagccg ttatatccat ttaactatgg ggacagaggg
     4381 cgcgattgta ccggcgcttg gctcgcccgg gcagcgtccc gcaaggcggg cgcgccgtgc
     4441 ggcggccctt cagaggcgcg ccagcatcca gggaatcgcc gccagcgagg cgacgaagcc
     4501 ggttgcgtgc gcgaggctcc accagccgaa acgccgaacc agatcgtccg gatcgatatt
     4561 ggcgatgaga aatggccgct tgtccgccgg cgcgcggacg atgtgcgcgt cggcgcggtc
     4621 gcgtgcctcg cggtgggcgc gggcgacttc gcggcgcgcc gccgaccgcg ccagcgacca
     4681 ttcgcgctgg tcgatctggc cattcgcgtc aaggtcgaat cgttcgtgca gggcctcgcg
     4741 gtcgcgcttc cattcggcga gcagttgcgc ggtatcgtcg ttggcgtcga gatcgaggct
     4801 gtggccgccg atcgtcttca gctcgccgag cacgtacagc gggtcgccgg cgatcagctt
     4861 ccattcggtg tggcggtaat cgccgtcgtg ccagcgctcg cggtggcggg tgatgacctc
     4921 ggctcggtcg gggtcgatca ccgcttcgcc gctgtcgtcg gcgaggatga ttgagtcgtc
     4981 gctctgccgt tcctggacga cctcccactt gttgtcggcg ctcttctgct cggtgcggta
     5041 gcggtaccag aggcagggca ggccagtcag cggcgaagcc accggattgc cgtcgaacag
     5101 ccggccgcgg ccgaccagct cgacgaatcc ctgcggcgcc gtcgccactt tggccgttgg
     5161 cgtgtcgacg atgatgcgcc gccgcctgag cgcgagaatc catccgtaga gactgagcgc
     5221 cgcgatcagc gcgaggacga cggtccacaa cactggtgaa gcgtcgacgc gggcgtgggc
     5281 aacgaaacca aaggcgagca gcgccagctg accacccgac gcgacgaact ggtcgagggg
     5341 acgctgcagg tccagcttca ttcagcagcg ggctctattc ggtgaacagc ttgcgcaggt
     5401 cgacgtcggc ggtttcggtc gaggaaaagc gcagcagtgc ctgccgctcg aagccgaaca
     5461 ggcgggcgac gatcagatcg gggaaggtat cgacgcggac gttgttgatg ttgaccgatt
     5521 cgttgtagag ctcgcggcgg tcggcgatcg cgttttcgag gccgcttatg cggctctgca
     5581 gctgcatgaa ctgctcgttg gccttgagct ccggataggc ttccatgacc gcgaagatct
     5641 gcccgaggcc ggcccgcagc atgccttcgg cggcgccgag cgccgggatg tcgtggcgtt
     5701 cgcgcgccga ctggacgcgg gtgcgggctt cggtcacgcg ctgcagcgtc tcttgttcga
     5761 actgcttgta ctgtcggcag gcctcgacca gctttggcag ttcgtcgtga cgctgcttga
     5821 gcatcacgtc gatgttcgcc caggccttct cgacggcgtt tcgcagcgac acgaagccgt
     5881 tgtagacgac gacgccgtag acgagggtga cgacgagcaa tgccagaaac accatcgtcg
     5941 agatgctcat ttccattgtt gttctcccta gcggtaagac gccgcgtcgc ggcgctggaa
     6001 tcgaatcagg gcgctgttgt ggcgtccgtc tcggctgctt cgggcggcat gcccagccgc
     6061 tcgcgcgtcg ccgcttcgtt gaggttgagc agggtgccga tggccttgat gtcgtcggcg
     6121 attgccgcgt tctcccagcc accctgcgcg agatggcgag ctagatcgac cgccagcgaa
     6181 acattgcgga cgcgcggatg gtcggccttg gtgtcgtcca tcaacaggcg cagcagttcc
     6241 ggcaactgcc agacctggca cagcgcgagc tgcagttcct gggtggtgat gccgaggacg
     6301 gccttttgcg cgacgacgct gcgcagctgg gagttggcct gttgcaggcg gtgaatctcg
     6361 agcatcagtt tcggcgcaaa ggtccacagc agcagttcgg cgatgtcgtg cagcagcgcg
     6421 gcgatagtga cctcctcggc attgaggtca tggcgccaga ttgcccagtc gcgggcgaat
     6481 tcggcggccc gctgggcacg ctggatggtc ttgagcacgc agagcaggac cgcgggctgg
     6541 tcggcgaggc gctgctcgac cagcggcagt tcggcgaagc ggcgaaagaa tggctcgatg
     6601 ccgagcatca tgattgcgct gcccacggtc gagatgtccc cgcgcaggga tttgccatgc
     6661 atcggctgga tgtaggcaag caccttcacc gtcatcagcg ggtcgcgcag caacaccgcc
     6721 gagaggtcgt ggcgggtgac ccggtcgatg cgctgcagcg ccgagtcgag cccgcgctgg
     6781 gtgacccgaa gggcagggat ctcggcgccg ttgaggtagg cgacgtagtc gtctaccgaa
     6841 gcgagggcgg cgtcgatcat cgtcgctggc ggctgccgct cagcggcccg gttcggcgag
     6901 tgccgggttc agggaatagg tgccggagat cgacgcctcg gcaaggatgt gcccggccat
     6961 cgccgcatgc acgtccttgg cggtgaaggc gccgtggacg atcagcttgg cggtgtcgag
     7021 tgcgtagagc gtgaactggt agtggtgccg gatgctgtcg ttccacggcg gacagggtcc
     7081 gtcgtagccg aagtaatcgc cacgcatgtc ggggtcgctg gcgaaccacc cggtgtaatc
     7141 gttgattccc tggcgggcgc cgtcggccgc attggggccg ggcttgccgc gggcgacgac
     7201 ctccttcgag aattcgccgg cggcgatctc gcgacggctt gccggcaggt cgacgagaac
     7261 ccagtggatg aaatcgacgc gcggcagact ggccggtatg gtccggcctt cctggttgac
     7321 gtcgtcgccg accgagggaa cgtcggggtc cttgcagatg accacgaagg actgcgtgcc
     7381 tggcggcacg tcgtcccagg ctagatgcgg attgcggttg ccgccgagac aggcgtgact
     7441 gcgctcgtcg ggaatgcaga aggcgaattc ccccggaatc gccgcgccat tgccaaagct
     7501 atcgctcgtc agtttcatgt gtcctcctcc gagaccttca aatatcggct cgccggcgga
     7561 aatcggcgag acggatgccg acgactctca aggctacgaa gtatacggca gccccgccgc
     7621 agaccagcgc gccgagtcgc catagccgct cgatcggcga gtagtgcagc cagctttcgg
     7681 cgttgccggc gaaaaaccag agtgcagccc ccatcaccgc cagtccggcc acgaggcggc
     7741 cgatgaaggc gagccagccg ggacgtggct ggtagatgcc gtgccggcgc aggccgcgat
     7801 acagaaatcc ggcgttgaaa caggccgcga gaccgatcgc cagcgccagc ccggcatgct
     7861 gcagcggcac gaccagcgcc aggttcagca cctgcgtgac ggccagcgag gcgagcgcga
     7921 tctttactgg cgtgcggatg ttctgccgtg cgtagaagcc cggggcgagg accttgacga
     7981 gcagcagccc ggtcagtccg acggtgtagg cgatcagcgc gcgttgggtc tggagcacgt
     8041 cggtggcggt gaaggcgccg tgatagaaca gcgtcgcgac caacggtgcc gagagaatcg
     8101 ccagtgccag cgccgccggc agcgccagca acagacacag gcgaaggccc cagtcgaggg
     8161 tctgcgaata ctcgtcgtgg cgttcctcgg ccgaaagccg cgagagcgtc ggcagcagaa
     8221 tcgtgccgag cgcggcgccg agcaggccaa ccggaaactc catcagccga tcggcgtagt
     8281 aaagccagga aacgcttccg gtctgcagga aggaagcgaa gatcgtattg atcagcagcg
     8341 aaatctgcgt caccgacacg ccgagcagcg ccggcgccat cagccgcatg acgcggcgca
     8401 cccccgggtc gcgccaggca agctgtggcc gcggcagcat gccgagtttc gccagcgccg
     8461 gcagctgcag cgacagctgt gcgaccccac ccagaatcac cgcccagccc agcgccatca
     8521 ccggttcggc gagccgtggc gcgagcagca gcgcgagggc gatgaaggac aggttgagca
     8581 gcaccggggt gaacgcggga atagcgaatc gcccccaggt gttgagtacg ccgccggcca
     8641 gcgccaccag cgacatgaac aggatgtagg gaaaggtcag ccgcgtcagt gacacggtca
     8701 gcgcgaactt gtcggcatcg gcggcgaatc ccggcgccga caggtagacc aaccaggggg
     8761 cggcgagcac gccgatgatg gagagcagca gcacgcacag gcccagcgtg ccggccaccc
     8821 gattgaccag tgcgtgggcg gcgtcatcgc cctggcggtt cttgtattcg ccgagaatgg
     8881 ggacaaaggc ctgcgagaag gccccttcgg cgaacattcg ccgcaacagg ttgggcagct
     8941 tgaaggcgac gaaaaacgcg tcggttgcca ccccggcgcc gaaagttcgg gcgatcacga
     9001 aatcgcggac aaagccgaga atccgggaga gcatggtcat gctgctcacg gtagccagcg
     9061 cgcggaggag gttcatcgct cgtctattgg tttcggcgga gtgttgcggt ggcggcgggg
     9121 cggccggggt cgcgaatcag ccgggatggt aacgaaatgc cgcgggtctg gcgcccccgg
     9181 tttccggaag cttccgcggg cgtcgcagaa ggct
//
LOCUS       DATDAA010000182         2489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_48643,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000182 DATDAA010000000
VERSION     DATDAA010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48643"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2042
                     /locus_tag="VIS73_06090"
     CDS             <1..2042
                     /locus_tag="VIS73_06090"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEY5762757.1"
                     /translation="AQIDVSIDATYQRVAKALANVEVESKREHYFKEFVWALRSGAIP
                     AGRIMSNAGALDHKPATSTINCTVSGTIRDSMDDILDKVHEAGLTLKAGCGIGYEFST
                     LRPRGAYVSGAGAYTSGPLSFMDIYDKMCFTVSSAGGRRGAQMGTFDVGHPDAADFIR
                     AKRENGRLRQFNLSLLVTDEFMQAVRDNGDWKLAFPLSVREYDPAEQDLADASLFVWR
                     EWPSTDGYVVNDDGLVACRVYKTLPARRLWDVIMASTYDFAEPGFVLIDRVNEMNNNW
                     WCENVRATNPCGEQPLPPYGSCLLGSVNLTRFVRKPFTAEAEFDWDEYREVVRVFTRM
                     LDDVVEINGLPLGRQREEIRRKRRHGMGFLGLGSTLTMLRMKYGSKESVAFTENVARE
                     MAVAGWETALELAREKGPAPIMADEFAVTREMLRKRPEMARDGWKVGDHIPGRILHAR
                     YSRYMQRLASGAPELVKQLESVGARFTHHTSIAPTGTISLSLANNASNGIEPTFAHHY
                     FRNVIRERRKTKERVDVYSFELLAYRELVNPRAMPNGTSASEQLPEYFVTAEDIGPRE
                     HVDIQAAAQKWVDSSISKTANVPTDFDYEQFKDIYLYAHERGLKGCTTFRFNPEAFQG
                     VLVKEEDLKNTTYVFTLEDGTEIEAKGDQEVEYDGEVHTAANLFDALKEGYYGRF"
     gene            2068..>2489
                     /locus_tag="VIS73_06095"
     CDS             2068..>2489
                     /locus_tag="VIS73_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762758.1"
                     /translation="MDKLNITKRIVKFRVRRPDAERAAAEREAPGREGRVRKDGAGKV
                     VWMHEKLERPEVLIGSTYKIRTPVSDHAMYVTINDIVLNEGTEHEHRRPFEIFINSKN
                     LDHFQWIVALTRILSAVFRKGGDVAFLIEELKAVFDPRG"
BASE COUNT          457 a          809 c          840 g          383 t
ORIGIN      
        1 gtgcccagat cgacgtgtcg atcgacgcga cctaccagcg agtcgcaaag gcactcgcca
       61 acgtcgaggt cgaatcgaag cgcgaacact atttcaagga attcgtgtgg gcgctgcgct
      121 cgggtgcaat ccctgcgggg cgcatcatgt cgaacgccgg cgcgctcgat cacaagcccg
      181 cgacctcgac catcaactgc accgtgtccg gcaccatccg cgactcgatg gacgacatcc
      241 tcgacaaggt ccacgaggcc gggctgacgc tcaaggccgg ttgcggcatc ggctacgagt
      301 tctcgacgct gcgcccgcgc ggggcgtacg tatccggcgc cggcgcctac acctcgggac
      361 cgctgtcgtt catggacatc tacgacaaga tgtgcttcac ggtgtcctcg gccggcggcc
      421 gccgcggcgc gcagatgggc accttcgatg tcggtcatcc cgatgccgca gacttcatcc
      481 gcgccaagcg cgagaacggc cgcctgcggc aattcaacct gtcgctgctg gtgactgacg
      541 agttcatgca ggcggtgcgc gataacggcg actggaagct ggcgtttccg ctctcggtgc
      601 gtgagtacga cccggccgag caggatctcg ccgacgccag cctcttcgtc tggcgggagt
      661 ggccgtccac cgacggctac gtcgtcaacg acgacggcct ggtcgcctgc cgcgtgtaca
      721 agacgctgcc ggcgcggcgg ctgtgggacg tcatcatggc gtccacctac gacttcgccg
      781 agcccggctt cgtgctcatc gaccgcgtca acgagatgaa caacaactgg tggtgcgaga
      841 acgtgcgcgc caccaacccc tgtggcgagc agccgctgcc gccgtacggc tcatgcctgc
      901 tggggtcggt caacctgacg cgcttcgtgc gcaagccgtt cacggccgag gcggagttcg
      961 actgggacga gtatcgcgag gtggtccggg tcttcacgcg catgctcgac gacgtcgtcg
     1021 agatcaacgg actgccgctc ggcaggcagc gcgaggagat ccgccgcaag cgccgccacg
     1081 gcatgggctt cctcggcctg ggctcgaccc tgaccatgct gcgcatgaag tacggctcga
     1141 aggagtcggt ggcgttcacc gagaacgtgg cgcgcgagat ggcggtcgcc ggctgggaga
     1201 ccgcgctcga gctggcgcgc gagaagggcc cggcgccgat catggccgac gagttcgcgg
     1261 tcacgcgcga gatgttgcgc aagcggccgg agatggcgcg cgacggctgg aaggtgggcg
     1321 accacatccc gggtcgcatc ctgcacgccc gctacagccg ctacatgcag cgcctggcca
     1381 gcggggcgcc cgagctggtg aagcagctcg agtcggtggg tgcgcgcttc acgcaccaca
     1441 cctcgatcgc gccgaccggc accatctcgc tgtcgctggc caacaacgcc agcaacggca
     1501 tcgagccgac ctttgcgcac cactacttcc gcaacgtgat tcgcgagcgg cgcaagacca
     1561 aggagcgtgt ggacgtctac tccttcgagc tgctggcgta ccgcgagctg gtcaaccccc
     1621 gcgccatgcc gaacggcacc agcgcatcgg agcagttgcc ggagtacttc gtcacggccg
     1681 aggacatcgg tccgcgcgag cacgtggaca tccaggcggc ggcgcagaag tgggtggatt
     1741 cgtcgatctc caagacggcc aacgtgccga ccgatttcga ctacgagcag ttcaaggaca
     1801 tctacctgta cgcgcacgag aggggactga agggctgcac gacgttccgc ttcaaccccg
     1861 aggccttcca gggcgtgctg gtcaaggaag aggacctgaa gaacacgacc tacgtgttca
     1921 cgcttgagga cggcaccgag atcgaggcca agggtgacca ggaagtcgag tacgacggcg
     1981 aggtccacac cgcggccaac ctgttcgacg cgctgaagga aggctattac gggaggttct
     2041 gaggcgctgc agcgcccggg acgcgacatg gacaagctga acatcaccaa gaggatcgtc
     2101 aagttccggg tgcgccggcc ggacgccgaa cgggctgcgg ccgagcgcga ggcgccgggc
     2161 cgtgagggcc gggtccgcaa ggacggcgcc ggcaaggtcg tgtggatgca cgagaagctc
     2221 gagcgcccgg aggtgctgat cggctcgacc tacaagatcc gtacgccggt ttccgaccac
     2281 gcgatgtacg tgacgatcaa tgacatcgtg ctcaacgagg gcaccgagca cgagcaccgg
     2341 cggccgttcg agatcttcat caactcgaag aacctggatc acttccagtg gatcgtcgcg
     2401 ctgacgcgga tcctgtcggc cgtgttccgt aagggcggcg acgtcgcgtt cctgatcgag
     2461 gagctgaaag cggtgttcga cccgcgcgg
//
LOCUS       DATDAA010000183         2017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_468648,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000183 DATDAA010000000
VERSION     DATDAA010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468648"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..>2017
                     /locus_tag="VIS73_06100"
     CDS             85..>2017
                     /locus_tag="VIS73_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762759.1"
                     /translation="MKHWRPPGSGGVALGLLAGITLAVSSTPASAVANCARQLTADVV
                     AIDMPIVNSRLGAATVNGMMYALRGDVVPISTTNADIAAVLIDNQVDPETGAITTVQR
                     RNAKLREDKRPRPLVLRVAAGDCLEVKFQNLVAPVPNPLNAPVDRDGIGNDPNPVTAD
                     GLPQIPVFVDEQVAERRVGFHVAGMQLRALGGIANDGSFVGKNASSLVAPGYSMTYNL
                     FAEKEQVYQVINPAALVGSDANQGNTANGLFGQIIVEPKGARIYRNIVTEEEMRLATR
                     HNDAVTNWCPTLDPMQAGTKAVKQVIGKGKKVREVTVEVPTYYCRTLDGHPILDYEAT
                     YPDIAPWNQEGKAGLPVLNMIQGTKIVHTEVDAIVAGPKPDGTFPPETYPLESVGKRN
                     PAYPNRLEAFRDFAQVWHDEVATAQAYPGFYSTGFRPPAGQDADPLTDVFAYLLKGVR
                     DKFMINYGSGGIGTEILSNRLGVGPMHDCLNCAYEEFFLTHFTVGEFGVLTDIPANAG
                     LETITPQNVLNILLAIKAGTTATALSTAELEFLAALGPKASKAFYPGDPANINYSYVG
                     DAVKFRNTHNGFEQHVFHLHNHQWLFNANDDNANYIDAQGIGPGMGYTYEIANGGSGN
                     RNKSSGDAIYHCHFYPHFAQ"
BASE COUNT          406 a          672 c          593 g          346 t
ORIGIN      
        1 aagtttcccg agtcagctgt tcagggggct cctaccggtc gctttgtaaa tcgtagttac
       61 ttctttattt aagggaggca gaccatgaaa cattggagac cacccggttc cggcggcgtc
      121 gccttgggac tgctggcagg catcacgctg gcagtctcgt cgacgccggc cagcgccgta
      181 gccaattgcg cccgccagct cacggcggac gtggtcgcga tcgatatgcc gatcgtgaac
      241 agccgacttg gcgccgccac cgttaacggg atgatgtatg ccctgcgtgg cgacgtcgtc
      301 ccgattagca ccaccaacgc cgacatcgcg gcagttctca tcgacaatca agtcgatccg
      361 gaaaccggcg ccattacgac cgtccagcgc cgcaacgcaa agctgcgcga agacaagcgt
      421 ccgcgtccgc tggtgctgcg cgttgcggcc ggagactgtc tggaagtcaa attccagaac
      481 ctggtcgcgc cggtgccgaa tccgctgaac gcccccgtcg atcgcgacgg catcggcaat
      541 gaccccaacc cggtcaccgc cgacggcctg ccgcagatcc cggtgttcgt ggacgagcag
      601 gtcgccgagc gtcgcgtcgg cttccatgtc gccggcatgc agttgcgggc tctgggtggc
      661 atcgccaacg acggttcctt cgtcggcaag aacgcctcga gcctggtggc tccgggttac
      721 agcatgacct acaacctctt cgccgagaag gagcaggtct atcaggtcat caacccggcc
      781 gcactggtcg gttccgatgc caaccaaggc aacacggcga acggcctgtt cggtcagatc
      841 atcgtcgagc ctaagggcgc gcggatctac cgcaacatcg tcaccgaaga agaaatgcga
      901 ctggcgacgc ggcacaacga tgcggtcacg aattggtgtc cgacgcttga tccgatgcag
      961 gccggtacca aggcggtcaa gcaggtgatc ggcaagggca agaaggtgcg ggaggttacc
     1021 gtcgaggtgc cgacctacta ctgccgcacg cttgatggtc acccgattct cgactacgag
     1081 gccacctatc cggatatcgc cccgtggaac caggaaggca aggccggtct accggtgctg
     1141 aacatgatcc agggcaccaa gatcgtgcac accgaagtcg atgcgatcgt cgcaggcccg
     1201 aagcccgatg gtaccttccc gcccgaaacc tatccgctgg aatcggtcgg caagcgcaac
     1261 ccagcctacc cgaaccgcct tgaggccttc cgtgactttg cacaggtttg gcacgacgag
     1321 gtggcgacgg cgcaggccta cccgggcttc tacagcaccg ggttccggcc gccggccggg
     1381 caggacgccg atccgctgac cgacgtcttc gcctacctgc tcaagggtgt gcgcgacaag
     1441 ttcatgatca actacggctc cggcggcatc ggtaccgaga tcctctcgaa ccggctcggc
     1501 gtcggcccga tgcacgactg cctgaactgt gcctacgagg aattcttcct cacgcacttc
     1561 acggtcggcg aattcggcgt attgaccgat atcccggcca acgcgggtct ggagacgatt
     1621 accccgcaga acgtgctgaa catcctgctc gcgatcaagg cagggactac tgccaccgcc
     1681 ctgtcgaccg ccgagttgga gttcctcgcc gcactcggcc cgaaggccag caaggccttc
     1741 tacccgggcg acccggccaa catcaactac agctacgtcg gcgatgcggt caagttccgc
     1801 aacacccaca atggcttcga gcagcacgtc ttccacttgc acaaccacca gtggctgttc
     1861 aacgcgaacg acgacaacgc gaactacatt gacgcccagg gcatcggccc tggcatgggc
     1921 tacacctacg agatcgccaa tggcggttcg ggtaaccgca acaagtcctc gggcgacgcg
     1981 atctaccact gccacttcta cccgcacttc gcgcagg
//
LOCUS       DATDAA010000184         5714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_503245,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000184 DATDAA010000000
VERSION     DATDAA010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503245"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /gene="fliM"
                     /locus_tag="VIS73_06105"
     CDS             <1..819
                     /gene="fliM"
                     /locus_tag="VIS73_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEY5762760.1"
                     /translation="YLRIGLFNYVHRNAEISVGPIRVQKYSEFIRNLVVPTNLNLVSI
                     KPLRGTGLIVFDPNLVFLVVDNMFGGDGRFHSRVEGRDFTMTEQRIIQGMLGVVFSEY
                     QKAWKPVFELQFDYVRSEMNSQFANIATPSEIVVATTFTLEFGGAAADMHICMPYAMV
                     EPIRDMLYSAMQSDQISSDQRWVKMLRNQLKDADVELVTQFTHTTMSLRQILKLKAGD
                     VIPVTLPDTVVTTIDDTPVMECRYGKQNGKLALRVERMIQSAADDEPQLGGRNG"
     gene            812..1219
                     /gene="fliN"
                     /locus_tag="VIS73_06110"
     CDS             812..1219
                     /gene="fliN"
                     /locus_tag="VIS73_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEY5762761.1"
                     /translation="MADSTDNQNDTVSEDDWAAAMAEQAQSEPAKPADVFQNFGGGAA
                     GGGLMNELEMILDIPVQISVELGRTKINIRNLLQLAHGSVVELDALAGEPMNVLVNGT
                     LIAQGEVVVVNDKFGIRLTDIITPSERMRKLGR"
     gene            1381..1803
                     /gene="fliO"
                     /locus_tag="VIS73_06115"
     CDS             1381..1803
                     /gene="fliO"
                     /locus_tag="VIS73_06115"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEY5762762.1"
                     /translation="MHPLRAILLAIPAASTSALAQNPSVPGVGTESFIQGFVGLAVIV
                     GLILAAAWLLRRFNVVGVTAGAAGMKIIAGLSVGPRERILLLEIGEHWIVVGVTSTQM
                     RTLHTLPKGKLPNGDGTGAPNPAAFSRLLDQFRRRDGA"
     gene            1790..2590
                     /gene="fliP"
                     /locus_tag="VIS73_06120"
     CDS             1790..2590
                     /gene="fliP"
                     /locus_tag="VIS73_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990285.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEY5762763.1"
                     /translation="MTAPDAMALLRRLASALRAPRGLLLIGAAAALGVAAPALAQQGL
                     PLVTSTPGPGGSQSFTLPIQTLITLTALTFIPAIVLMMTGFTRIIIVLALLRQAIGTP
                     TAPPNQVVLGLSLFLTFFVMSPVIDRVYTEAYLPLTENRINFNQALERAAVPVRGFML
                     KQTRESDLALFARLAETGPIASPEETPMRVLVPAFVISELKTAFQIGFLIFIPFLVID
                     MVVASVLMSMGMMMMSPIMVALPFKLMLFVLVDGWNLVVGSLVQSFAR"
     gene            2595..2864
                     /gene="fliQ"
                     /locus_tag="VIS73_06125"
     CDS             2595..2864
                     /gene="fliQ"
                     /locus_tag="VIS73_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEY5762764.1"
                     /translation="MTPETVVEVGRQALYVLLLVSGPMLLAALGIGLIVSIFQAATQI
                     NEATLSFIPKLFGVFFALIFAGPWMLELLVDYIRRLFESIPQIIG"
     gene            2884..3657
                     /gene="fliR"
                     /locus_tag="VIS73_06130"
     CDS             2884..3657
                     /gene="fliR"
                     /locus_tag="VIS73_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEY5762765.1"
                     /translation="MIAFTTAELYALIAAVLYPLARVLAFMSIAPLFNNNAVPMRIRL
                     LIGVAITIGIAPLVPPIPAIEPSSAPGLLMLAREMLIGFSMGFAMRIVFAAISMAGEQ
                     IGMQMGLGFAVSYDPLNTSQTPVMAEFLALITTLIFLSINGHLMMVATLAQGFQVLPI
                     SLDGLPPTAWLNIVLHGKVIFATGMLLALPVTGAMIITNLSLGVLNRAAPQLNLFAIG
                     FPVTIAGGFVILLLTLNHLVPPLQRLFEAGLQMMLAFPG"
     gene            complement(3942..>5714)
                     /locus_tag="VIS73_06135"
     CDS             complement(3942..>5714)
                     /locus_tag="VIS73_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762766.1"
                     /translation="TSITVSTRSRARFLILLAGVTSAFLVACGGGGSTQPAGLAASLQ
                     PEDGAVAYVAMAEVSTVVSTAATGPGYELVWANTPGGVSVATDAADNVYSVLWDYNRA
                     GDIYLTKRNVDGVLQWEVRFDNTDLSRHEVATWVDTDSQGNVLVSGTIRSGYSKPVNA
                     NSVLMKFAPDGRLLWRQVYATDFDGSSTRKLLIDNADNVYVLGLGRSPSGQRTTVRKF
                     RSDGSTEWDWFDPVGIGNPLNFKWAADGDLVIAARTYFGSLNGYAKVDRSGSTVWALS
                     AFYSPTAGDAAGDAAGNTYLINGSDSATGSVLRKLGPGGATLWERTHSLAGLRVEVGS
                     DNSPVVSGFPNRVTAGAAFVKFDPSGNLLWANLDADGPGVALLAHSQMRLDSLNNAYL
                     VAGNLSQMGVTKVLSDGSSAWTALVPFGYGYGMAFGSNQQVFVAGGTVARIDQAGGGD
                     LPPPPPPTEASADLALSLSDSPDPVRSKEALVITATIENHGPSAATRVNFGAMLPEGE
                     TLLSVVTSNGSCSGNTVISCALDTIAFGEVATVTMTLHVAHTSGILTTTASVNASEID
                     PVVGNNTAQVTTQVVKPRRFRPRR"
BASE COUNT          975 a         1948 c         1748 g         1043 t
ORIGIN      
        1 tacctgcgaa taggcctgtt caactacgtc caccgcaatg cggagatctc ggtcggcccg
       61 atccgcgtgc agaagtacag cgaattcatc cgcaacctcg tggtgccgac caaccttaac
      121 ctggtctcga tcaagccgct gcgcggcacc ggcctgatcg tcttcgaccc aaacctggtc
      181 tttctcgtcg tcgacaacat gttcggcggc gacgggcgat tccactcgcg tgtcgagggg
      241 cgagatttca cgatgaccga gcagcgcatc atccagggca tgctcggcgt cgtgttcagc
      301 gagtatcaga aggcctggaa gccggtcttc gagctgcagt tcgactacgt ccgctcggag
      361 atgaattcgc agtttgccaa cattgccacg ccttccgaga tcgtcgtcgc gacgaccttc
      421 acgctcgaat tcggcggcgc tgcggccgac atgcacatct gcatgcccta cgcgatggtc
      481 gaaccgatcc gcgacatgct ctacagcgcg atgcagagcg accagatctc cagcgaccag
      541 cgctgggtga agatgctgcg caaccaactc aaggacgccg atgtcgagct ggtcacgcag
      601 ttcacgcaca cgacgatgtc gttgcggcaa attctcaagc tcaaggccgg cgacgtgatt
      661 ccggtcacgc tgccggacac cgtcgtgacc acaatcgacg acacgccggt catggaatgc
      721 cgttacggca agcagaacgg caagctcgca ttgcgggtcg agcggatgat ccagtccgcc
      781 gccgacgacg agccccaact aggaggcagg aatggctgat tccaccgaca atcagaacga
      841 cactgtaagc gaggacgact gggccgcagc aatggccgag caggcgcaga gcgaacccgc
      901 caagccagcc gatgtcttcc agaacttcgg cggcggcgcg gccggcggcg gcctgatgaa
      961 cgaactcgag atgatcctcg atatcccggt gcagatctcg gtcgaactcg ggcggaccaa
     1021 gatcaacatc cgcaacctgc tgcagcttgc ccacggctcg gtcgtcgaac tcgacgcact
     1081 ggccggggag ccgatgaacg tactggtcaa cggcacgctg atcgcgcagg gtgaggtcgt
     1141 cgtcgtcaat gacaagttcg gcatccgcct caccgacatc atcacgccct ccgagcgcat
     1201 gcggaaactt ggccgctaag ttttactgag ttcggtcgcc gagttcagcc accaggctcg
     1261 gccgccgagt cccggcggcc gccagcgcct gtccatggca accagccacg cccgcgaccg
     1321 gcacgcccgc gcgggcgtgc ttgctttaga atcgcggcat cctcacgacc cggtcccgcc
     1381 gtgcacccgc tccgcgcaat cctcctcgcc attccagcgg catccacgtc agcccttgca
     1441 caaaacccca gcgttcccgg cgtcgggact gaaagtttca tccagggctt cgtcggcctc
     1501 gccgtcatcg tcggcctgat cctcgccgcc gcctggctcc tgcgccgttt caatgtcgtc
     1561 ggcgtcaccg cgggtgccgc cggcatgaag atcatcgccg gtctctcggt cggcccgcgt
     1621 gaacgcatcc tcctgctgga aataggcgaa cactggatcg tcgtcggcgt cacctcaacc
     1681 cagatgcgca cgctgcacac gctgcccaag ggcaaactgc ccaacggcga cggaaccggc
     1741 gcacccaatc cagccgcctt cagccggctg ctcgaccagt tccgtcgccg tgacggcgcc
     1801 tgacgcgatg gccttgctga ggcgactggc cagcgccctg cgcgcaccgc gcggcctcct
     1861 gctcattggc gcagcggcgg cgcttggcgt tgccgcgccg gcactggcgc agcagggact
     1921 gccactggtg accagcaccc cgggcccagg cggcagccag tcgttcacgc tgccgatcca
     1981 gacgctgatc acgctgacgg cgctgacctt catcccggcg atcgtgctga tgatgaccgg
     2041 cttcacccgc atcatcatcg tcctcgcgtt gctgcgacag gcaattggca cgccgaccgc
     2101 gccgcccaac caggtggttc tcggcctctc gctctttctc acgtttttcg tcatgtcgcc
     2161 ggtgatcgac cgcgtctaca ccgaggccta cctgccgctt accgaaaacc gcatcaactt
     2221 caaccaggcg ctggagcgcg ccgcggtacc ggtgcgcggc ttcatgctca agcagacccg
     2281 cgaatccgac ctcgcgctgt tcgcccggct tgccgaaacc gggccgatcg cctcacccga
     2341 agagacgccg atgcgggtgc tggtgcccgc cttcgtgatc agcgagctga agaccgcctt
     2401 ccagatcggc ttcctgatct tcatcccctt tctcgtcatc gacatggtcg tcgccagtgt
     2461 gctgatgtcg atgggcatga tgatgatgtc gccgatcatg gtcgcgctgc cgttcaagct
     2521 gatgctcttt gtcctcgtcg acggctggaa tctcgtcgtc ggttctctcg tccaaagttt
     2581 cgcgaggtag cgccatgacc cccgagaccg ttgtcgaagt cggccgccag gcgctctacg
     2641 tactgctgct ggtttccggg ccgatgctgc tggctgcact cggtatcggc ctgatcgtga
     2701 gcatcttcca ggcggcgacg cagatcaacg aagccacgct gtcgttcatc cccaagctct
     2761 tcggcgtctt cttcgcgctg atcttcgccg gcccgtggat gctcgagctg ctcgtcgact
     2821 acatccggcg gctgttcgaa agcattccgc agatcatcgg ctgagcaccc cggtcgccct
     2881 gccgtgatcg ccttcaccac cgccgagctt tatgcgctga tcgccgcggt gctctacccg
     2941 ctggcgcgcg tgctcgcctt catgagcatt gccccgctgt tcaacaacaa cgcggtgccg
     3001 atgcgcatcc ggctgctaat cggcgtcgcc atcaccatcg gcatcgcgcc gctggtgccg
     3061 ccgattccgg cgatcgagcc gtcctcggca ccgggcctcc tgatgctcgc ccgagaaatg
     3121 ctgatcggct tttcgatggg tttcgcgatg cgcatcgtct ttgccgcgat cagcatggcc
     3181 ggcgaacaga tcggcatgca gatgggcctc ggcttcgcag tctcctacga cccgctcaac
     3241 acctcgcaga cgccggtgat ggccgaattc ctcgcgctga tcacaacgct gatctttctc
     3301 tcgatcaacg gccacctgat gatggtggcg acgctggcgc agggcttcca ggtgctgccc
     3361 atctcgctcg acggcctgcc gccgacggcc tggctcaaca tcgtcctgca cggcaaggtc
     3421 atcttcgcca ccggcatgct gctggcgcta ccggtgaccg gcgcgatgat catcaccaac
     3481 ctttcgctcg gcgtgcttaa ccgcgccgcg ccgcaactta acctcttcgc catcggcttt
     3541 ccggtcacca ttgccggcgg cttcgtcatc ctgctgctga cgctcaacca cctcgtgcca
     3601 ccgctgcagc gactgttcga ggccgggttg cagatgatgc tggcgtttcc gggctagggc
     3661 cggaaacgct gattctgcac gggctgccgg caggtcagac ccgcgactga aggcaagaag
     3721 gcgagttgaa tcctgtgcgg gagtccgcct tccgcgggaa aggccttcgc gctaaaaaca
     3781 cgagcttgat cgatgggccg ccccggctcc acagccacga gcaggatgcg tggctgtagc
     3841 ggcgggcatc aggcgccgcc ggcgcagttc acgctccaca ggcgaccact tcggcgtcga
     3901 cgacgaccga ggcggccccc ggacggcgcc gggacggttg ctcagcgccg cgggcggaaa
     3961 cgccgcggtt tgacgacctg ggtggtcacc tgtgccgtgt tattcccgac caccggatcg
     4021 atttcgctgg cgttgacgct ggcggtggtg gtcagaatac cgctcgtgtg cgcgacatgc
     4081 agcgtcattg tcacggtcgc cacctccccg aaggcgatgg tgtcgagcgc gcaactgatc
     4141 accgtgttgc cgctgcagga gccgttcgac gttaccacgc tcagcagcgt ctcgccttcg
     4201 ggcagcatcg caccgaagtt gacgcgggtc gctgccgaag gcccatggtt ctcgatcgtt
     4261 gcggtgatca ccaaggcttc tttgctgcgc accggatcgg gcgagtcgga aaggctcagc
     4321 gccaagtcgg ctgaagcctc ggtgggcggt ggcggcggtg gcaggtcgcc gccgcctgcc
     4381 tgatcgatgc gggcgaccgt accaccggcg acgaagacct gctgattgct gccgaaggcc
     4441 atgccgtagc cgtagccgaa gggcactagt gcggtccacg ccgaactgcc gtcgctgagc
     4501 acctttgtca cgcccatctg tgacaggttg ccggcgacga gatacgcgtt gttaagcgaa
     4561 tcgagccgca tctgcgaatg ggcaagcagc gcaacgcccg gtccgtcggc gtctaggttg
     4621 gcccacagca ggttgccgga tgggtcgaac ttgacgaagg ccgcaccggc ggtgacgcgg
     4681 ttcggaaagc cgctgaccac cggcgagttg tcgctgccga cctcgactcg caggccagcc
     4741 agcgaatgcg tgcgctccca caaggtggcg ccgccgggcc cgagcttgcg caacacgctg
     4801 ccggtggccg aatcgctgcc gttgatcaga taggtgttgc cagccgcgtc gccggccgca
     4861 tcgcccgctg tcgggctgta gaaagctgac agcgcccata cggtgctgcc gctgcggtcg
     4921 accttggcgt agccgttgag actgccgaag taagtacgcg cggcgatcac taggtcgccg
     4981 tcggccgccc acttgaagtt caaggggttg ccgattccta ccggatcgaa ccagtcccat
     5041 tcggtgctgc cgtcgctccg gaacttgcgc accgtcgtcc gttggcccga gggtgacctg
     5101 cccaagccca gcacgtagac gttgtcggca ttgtcgatca gcagcttgcg cgtcgacgaa
     5161 ccgtcgaagt cggtggcgta gacctggcgc cagagcaagc ggccatcggg cgcaaacttc
     5221 atcagaacgc tgttggcgtt gaccggtttc gagtaacccg agcggatcgt tccgctgacg
     5281 aggacgttgc cctggctgtc ggtatcgacc caggttgcca cctcgtgccg cgacaggtcg
     5341 gtattgtcaa atcgcacctc ccactgcagc accccgtcga cgttgcgctt ggtcaggtag
     5401 atgtcgcccg cccggttgta gtcccacagc acgctgtaca cattgtcggc agcgtcggtg
     5461 gccaccgaaa caccacccgg cgtgttggcc cagaccagtt cgtagcccgg gccggtcgcg
     5521 gcggtagaaa cgaccgtgct gacctcagcc atcgccacgt aagccactgc gccatcttcc
     5581 ggttgcagcg aagcggcgag cccggctggc tgggtcgaac cgccaccacc gcaggccacg
     5641 aggaaggcgg acgtgacacc ggccaacagt atgaggaagc gagcgcggct gcgtgtggaa
     5701 actgtgatcg atgt
//
LOCUS       DATDAA010000185         2258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_48752,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000185 DATDAA010000000
VERSION     DATDAA010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48752"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VIS73_06140"
     CDS             complement(<1..837)
                     /locus_tag="VIS73_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY5762767.1"
                     /translation="MNATPPASIATALWASKPLPQKALATIVLAGFVLQGLLSAIECV
                     GQFLADSSSGTALWQSLFAGLATAIGAVLLATLQRPSPRGLGIAMAIAAGAMSMAAIL
                     TLLIPASQHEAASPTTIALALGVGAALMFLIDRALPHEHPQPDAKRLPATASRRAAWL
                     LVVAITLHNFPEGFAVAAAAAGTGGTVTATAIALQNIPEGLIIAAVLWAIGLRRSVAI
                     GIAAASGLAEPVGALLGIVWLDAIPGAAPSAMALAAGAMLFVVAHELLPTAVRALASW
                     RAG"
     gene            complement(967..>2258)
                     /locus_tag="VIS73_06145"
     CDS             complement(967..>2258)
                     /locus_tag="VIS73_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5762768.1"
                     /translation="LPNRLLAQDRLAQAMANADRAGHQTKVALLFIDLDDFKKINDTL
                     GHDHGDELLKQVAERFKTCVRKTDTVARFGGDEFLVIVTSVRDPANVELVAENLLRQM
                     TTPFQLGGVSFVINASIGIALYPDDGTDVAHLLQDADSAMYRAKREGRGQFRFFTAAM
                     NAEMLEHLQLEEGLRHAIERKELEVHFQPAIDCRDGSIVGAEALLRWNHPELGMVPPV
                     KFIPLAERSNLILPIGEWVLRESCRIAAGWAKASQGRFRIAVNLSAKQFGGQRILRQV
                     QAALAESGLPPENLELEFTESVLMREDEETMQVIRELHKMGIRFAIDDFGTGYSSISY
                     LRQYPFDTLKIDRSFVNDVVTNPDDANLVRSIVALASSLQLEVLAEGIEEWPQFDLLQ
                     EAGVDRCQGWLFSKALPADAFSAQMANWPGLSRKPSA"
BASE COUNT          402 a          701 c          777 g          378 t
ORIGIN      
        1 gccggcgcgc caactcgcca gcgcccggac cgccgtcggc agcagttcgt gcgcgacgac
       61 gaagagcatc gcgccggccg ccagcgccat cgctgatggt gcagcgccgg gaatcgcgtc
      121 gagccagacg atgcccagca gcgcgccgac tggctcggcc agcccgctgg ccgcggcgat
      181 gccgatcgcg acacttcggc gtagcccgat tgcccagagg acggcggcaa tgatcagtcc
      241 ttcgggaatg ttctgcagag cgatcgctgt cgcggtgacg gtgccgcccg tgccagccgc
      301 ggcggcggcg accgcgaagc cttcgggaaa gttgtggagc gtgatcgcga caacgaggag
      361 ccacgcagcg cgtcgcgaag cggtggccgg cagacgcttg gcatcgggtt gcggatgctc
      421 gtgcggcaac gcgcgatcga tcaggaacat gagcgctgcg ccgacaccca gggccagggc
      481 gatcgtcgtc ggcgacgccg cctcgtgctg cgaggccgga atcagcaggg tgaggattgc
      541 cgccatactc atggccccgg cggcgatggc catcgcgatg ccgagcccgc gcggcgacgg
      601 acgctgcagc gttgccagca ggactgcgcc gatggcagtg gccaatccgg cgaacaagga
      661 ttgccagagc gccgtgcctg aggacgaatc ggcgaggaac tgacccacac attcgatcgc
      721 cgacagcaaa ccctggagca cgaagccggc gaggacgatc gtggctaagg ccttctgcgg
      781 caggggtttg ctggcccaga gggcggtggc gatactggct ggcggcgtgg cgttcatgtc
      841 gtgccccgtg tgacttggct acgaccttat tttcggcgct gcgcctgaaa atgtgaaatc
      901 gattgacgga atcgaatcga tagagaaaac caatgagatc cgccttgtgg cgtgacggtg
      961 acggccttac gcggagggct tgcgggagag gccgggccag ttcgccatct gcgccgaaaa
     1021 cgcgtctgcc ggaagcgcct tgctgaacag ccagccctga cagcgatcga cgccggcctc
     1081 ctgcagcagg tcgaactgcg gccattcctc tatgccttcg gcgagcacct caagctgcag
     1141 gctgctggcg agggcgacga tagagcggac gagattggca tcgtcggggt tggtgaccac
     1201 atcgttgacg aagctgcggt caatcttcag cgtgtcaaaa gggtattgcc gcagatagct
     1261 gatcgacgaa tagccggtgc cgaaatcgtc gatcgcgaaa cggatgccca tcttgtgcag
     1321 ctcgcggatg acctgcatgg tttcctcgtc ctcgcgcatc aacacgctct cggtgaactc
     1381 gagctcgagg ttctcgggcg gcaggccgct ttcggcgagc gcggcctgga cctggcgcaa
     1441 gatgcgctgg ccgccgaact gcttggccga caggttgacg gcgatgcgga agcgcccctg
     1501 gctggccttc gcccatccgg cagcgatgcg gcacgactca cgcagcaccc actcgccgat
     1561 cggcaggatc aggttgctac gctcggccag cgggatgaat ttcaccggcg gcaccatgcc
     1621 gagttcggga tggttccagc gcagcagcgc ttcggcgccg acgattgagc catcgcggca
     1681 gtcgatcgcc ggctggaagt ggacctcgag ctccttgcgc tcgatggcat ggcgcaagcc
     1741 ctcttcgagc tgcaggtgtt cgagcatctc ggcattcatc gccgccgtga agaaacggaa
     1801 ctgtccgcgg ccttcacgct tggcgcggta catcgccgaa tcggcgtcct gcagcagatg
     1861 tgccacatcg gtgccgtcgt cgggatagag cgcaatgccg atgctggcgt tgatgacgaa
     1921 gctgacaccg ccgagctgaa agggcgtcgt catctggcgg agcaggttct cggcgacgag
     1981 ttcgacgttg gccggatcgc ggacactggt gacgatcacc aggaactcgt cgccgccgaa
     2041 gcgggcgacc gtatcggtct tgcgcacgca ggtcttgaaa cgttcggcaa cctgcttcag
     2101 caactcgtca ccgtggtcgt ggccgagggt gtcgttgatc ttcttgaaat cgtcgaggtc
     2161 gatgaagagc aaggccacct tggtctggtg tccggcacgg tcggcattgg ccatcgcctg
     2221 cgccagccgg tcctgcgcca gcagccggtt gggcaagc
//
LOCUS       DATDAA010000186         3950 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_119375,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000186 DATDAA010000000
VERSION     DATDAA010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119375"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VIS73_06150"
     CDS             <1..417
                     /locus_tag="VIS73_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEY5762769.1"
                     /translation="LKAKIQQVRHAVVPLMEAVGKLHSGRVPKVCGNSEHYFRDVFDH
                     LARINAALDRSRETIATAIQVSLSMVSIEQSEIGKRLAAWAGIFAVITAFVGIWGMNF
                     RHMPELEWRYGYPAALALIALVSGFLYRKFKRAGWL"
     gene            532..1977
                     /gene="pyk"
                     /locus_tag="VIS73_06155"
     CDS             532..1977
                     /gene="pyk"
                     /locus_tag="VIS73_06155"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY5762770.1"
                     /translation="MTRHTKIVATLGPSSSSPEVMEALLRAGVDVVRVNFSHGSAADH
                     VARVQAIRAVAEKVGRPVGILADLQGPKIRVGKFESGKVALSVGDAFILDAKCELGNQ
                     ERVGLDYKDLTRDVRKGDMLLLDDGRIVLEVGRVTSSEIHTIVRHGGDLSNNKGINRL
                     GGGLSAPALTSKDMDDIKTAASVGADFIAVSFPKSAADMYMARQLARAAGSRALTIAK
                     IERTEAVANLEEILDACDGVMVARGDLAVEVGDAAVPALQKKMIRMARERNKLTITAT
                     QMMESMILSPIPTRAEVSDVANAVLDGTDAVMLSAETAAGKYPVHTVESMARICLEAE
                     KSAEVTLDSQFLNRVFTRIDQSIAMAAIWTAHHLKVKAIAALTQSGSTALWMSRLNCG
                     VPVYALTPEAESQTKMCLFREVYPLLMAQTHTDRDELLYDAEQLLIKQGVVQKGDLIV
                     LTVGEPIGSTGGTNTLKIVRVGEYQPAAVAG"
     gene            2022..3086
                     /gene="fba"
                     /locus_tag="VIS73_06160"
     CDS             2022..3086
                     /gene="fba"
                     /locus_tag="VIS73_06160"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988217.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY5762771.1"
                     /translation="MPIVSMRQLLDHAAENSYGLPAFNVNNMEQVWAIMEAANQLDAP
                     VIMQASAGARKYAGEPFLRHQILAALEAYPHIPIVMHQDHGQSPAVCMAAIRSGFSSV
                     MMDGSLQADGKTVASYEYNVDVSREVVKFAHAIGVSVEAELGVLGSLETMKGDKEDGH
                     GADGHMTREQLLTDVEQAADFVKQTNCDALAIAIGTSHGAYKFTKKPTGDILAIDRIK
                     EIHARIPNCHLVMHGSSAVPQELLAEIRQFGGDMKETYGVPVEEVQEGIKHGVRKVNI
                     DTDIRLAMTGAIRRYFAENPSKFDPRDYLKPAREAAKKICLARYESFGCAGRASQIKP
                     VPLEKMAERYAKGELNQVVK"
     gene            3172..3852
                     /gene="tmpT"
                     /locus_tag="VIS73_06165"
     CDS             3172..3852
                     /gene="tmpT"
                     /locus_tag="VIS73_06165"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HEY5762772.1"
                     /translation="MEAGFWHGKWAKNEIGFHEKEANPLLVDHLQSLSLPNGARVFVP
                     LCGKSLDMHWLLAQGHRVVGVELSRLAVEQLFEELGAKPTVTRIGWLDRYAAPGIEVY
                     VGDLFRLSADLLGVVDAIYDRAALVALPAEMRGDYARHLIEISLRAPQLLICFEYEPH
                     LMAGPPFPVFSDEVLGRYQQDYDVDLRASVPIPGGFKGRIPATESVWLLCPRLSTPDR
                     LFPVLTHR"
BASE COUNT          770 a         1340 c         1190 g          650 t
ORIGIN      
        1 ttgaaggcga agatccagca ggtgcgccac gcggtggtgc cgctgatgga ggcggtcggc
       61 aaactgcaca gcggccgcgt gcccaaggtc tgcggcaaca gcgagcacta cttccgcgac
      121 gtcttcgacc accttgcccg catcaacgcc gcgctcgacc gcagccgcga gaccatcgcc
      181 acggcgatcc aggtctcgct gtcgatggtc tcgatcgagc agagcgagat cggcaagcgg
      241 ctcgccgcct gggccggcat cttcgccgtg atcaccgcct tcgtcggcat ctggggcatg
      301 aacttccgcc acatgcccga gctcgagtgg cgttacggct acccggcagc gctggcgctg
      361 atcgcgctgg tcagcggctt tctctaccgc aaatttaagc gtgccggctg gctctgagcg
      421 ctttgccggc gcgcgccggc tggcggagat gccccttctc tgcgggcgaa gggcgcaacc
      481 gctacaatcg cttctttccc aaaaacaatg aaaacccgct ccggagactg catgacccgc
      541 cataccaaga tcgtcgccac gctcggcccc tcctcttcct cgcccgaggt catggaggcc
      601 ctgctgcgcg ccggcgtcga tgtcgtccgc gtcaacttct cgcatggcag cgccgccgac
      661 cacgtcgccc gggtccaagc aatccgtgcg gttgcggaaa aggtcggccg cccggtcggc
      721 atcctcgccg atttgcaggg cccgaaaatc cgcgtcggca agttcgagag cggcaaggtc
      781 gcactcagcg tcggtgacgc cttcatcctc gatgccaagt gcgagctcgg caaccaggaa
      841 cgcgtcggcc tcgactacaa ggacctcacg cgcgacgtgc gcaagggcga catgctgctg
      901 ctcgacgacg gccgcatcgt gctcgaggtg ggccgcgtta ccagctccga aatccacacc
      961 atcgtccgcc acggtggcga cctgtccaac aacaagggca tcaaccggct gggcggcggc
     1021 ctttcggcac cggccctgac ctcaaaggac atggacgaca tcaagacggc agcctcggtc
     1081 ggtgccgact tcatcgccgt gtcgtttccg aagagcgcgg ccgatatgta catggcgcgc
     1141 cagctggcgc gcgcggccgg cagccgggca ctgaccatcg ccaagatcga gcgcaccgag
     1201 gcggtggcca accttgaaga gattctcgac gcctgcgacg gtgtgatggt cgcccgcggt
     1261 gacctcgccg tcgaagtcgg cgacgcggcc gtaccggcgc tgcagaagaa aatgatccgc
     1321 atggcgcgcg agcgcaacaa gctgacgatc accgccacgc agatgatgga gtcgatgatc
     1381 ctgtcgccga ttccgacgcg cgccgaagtt tccgacgtcg ccaacgccgt gctcgacggc
     1441 accgacgcgg tgatgctctc ggccgaaacc gctgccggca agtacccggt gcacacagtc
     1501 gaatcgatgg cgcgcatctg cctcgaagcc gagaagtcag ccgaggtcac gctcgacagc
     1561 cagttcctca accgcgtgtt tacccggatc gatcagtcga tcgccatggc cgcgatctgg
     1621 accgcccacc acctcaaggt caaggccatc gcagcgctga cccagtcggg ttcgacggcg
     1681 ctgtggatga gccgcctcaa ctgcggcgtg ccggtctatg cgttgacccc cgaagcagag
     1741 tcgcagacca agatgtgcct gttccgtgag gtttacccgc tgctgatggc gcagacacac
     1801 accgaccgcg acgagctgct ctacgatgcc gaacagctat tgatcaagca gggcgtggta
     1861 cagaagggcg acctgatcgt gctcaccgtc ggcgagccga tcggctccac cggcggcacc
     1921 aacaccctga agatcgttcg cgtcggtgag taccagccgg cggccgtcgc cggctgaccc
     1981 agccattcat aaccaaacga caagcacgag aggagaacac catgcccatc gtatcgatgc
     2041 gccaactgct cgaccacgcc gccgagaaca gctacggcct gcccgccttc aacgtcaaca
     2101 acatggaaca ggtgtgggcg atcatggaag cggcgaacca gctcgacgca ccggtgatca
     2161 tgcaggcctc ggcaggcgcc cgtaagtacg ccggcgaacc cttcctgcgc caccagatcc
     2221 tcgccgcgct cgaagcctat ccgcacatcc ccatcgtcat gcatcaggac cacgggcaaa
     2281 gccccgccgt gtgcatggct gcgatccgct cgggcttctc atcggtgatg atggatggct
     2341 cgctgcaggc cgacggcaag accgtcgcca gctacgaata caacgtcgac gtctcacgcg
     2401 aagtcgtcaa gtttgcgcac gccatcggcg tctcggtcga agccgaactc ggcgtcctcg
     2461 gctcgctgga gaccatgaag ggcgacaagg aagacggcca cggcgccgac ggccacatga
     2521 cgcgcgagca attgctcacc gacgtcgaac aggccgccga cttcgtcaag cagaccaact
     2581 gcgacgcgct ggccatcgcc atcggcacta gccacggcgc atacaagttc accaagaagc
     2641 ccaccggtga catcctggcg atcgaccgca tcaaggaaat ccacgcccgc atccccaact
     2701 gccacctcgt catgcatggc agctcggccg tgccgcagga actgctcgcc gagatccgcc
     2761 agtttggcgg cgacatgaag gaaacctacg gcgtgccggt ggaggaagtg caggaaggca
     2821 tcaagcacgg cgtgcgtaag gtcaacatcg ataccgacat ccgcctagcg atgaccggcg
     2881 ccatccgtcg ctacttcgct gagaatccga gcaagttcga cccgcgcgac tacctcaaac
     2941 cggcgcgcga agccgccaag aagatctgcc tcgcgcgcta cgaatccttc ggctgcgccg
     3001 gccgcgccag ccagatcaag ccagtgcctc tggagaagat ggccgagcgg tatgcgaagg
     3061 gcgagttgaa ccaggtcgtg aagtaagacg acaacagccg ctcacgaaga agccgcctgc
     3121 gggcggtttt tttatgaccc ccgatcatta aggacaggag acgcgaggtc catggaagca
     3181 gggttttggc acggcaaatg ggcaaagaac gaaatcggct tccatgagaa ggaggccaac
     3241 ccactgctgg tggatcattt gcagtcgctc tccctgccga atggcgcgcg cgtcttcgtc
     3301 ccgctgtgcg gcaagtcgct cgatatgcac tggctgctgg cccaaggcca ccgcgtcgtc
     3361 ggcgtagagc tcagtcgact ggccgtcgag cagttgttcg aagaactggg cgccaagccg
     3421 acggtcaccc gcattggctg gctggaccgt tatgccgccc ccggcataga ggtttacgtc
     3481 ggcgacctgt tccgcctgtc tgccgacttg ctgggcgtgg ttgacgcgat ctacgaccgc
     3541 gcggcactgg ttgcactacc ggcggagatg cgcggcgact atgcgcgcca tctgatcgaa
     3601 atttcattgc gtgcgccaca gctgctgatt tgtttcgaat acgaacccca cttgatggcg
     3661 ggcccgccgt tcccggtatt ctcggacgag gtcctcggcc gctaccagca agactacgac
     3721 gtcgatttgc gtgcgtccgt accgatacca ggcggcttca agggcaggat ccctgcgacc
     3781 gagagcgtat ggctcctatg cccccgatta tcgactccgg atcgcctctt tcccgtcctt
     3841 acccatcgat agtgtgcatg agcccgtcac cgctctgccc catcgacagt tcagccgcgg
     3901 cctcctgagt ccagcagcga catctgcctg ccgtagttcc catccgcgac
//
LOCUS       DATDAA010000187         5644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_363983,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000187 DATDAA010000000
VERSION     DATDAA010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363983"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2373)
                     /gene="uvrA"
                     /locus_tag="VIS73_06170"
     CDS             complement(<1..2373)
                     /gene="uvrA"
                     /locus_tag="VIS73_06170"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY5762773.1"
                     /translation="MEDLRIRGARTHNLQNIDLDLPRNRLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMEKPDVDLIEGLSPAISIEQKATSHNPRSTVGTVT
                     EIHDYLRLLFARAGTPYCPEHGEPLEAQTVSQMVDHVLELPAETRLSILAPVVANRKG
                     EQLDLFAELRAQGFVRLRINGEIHEIDALPKLAKSKKHTVDVVVDRLKVREDMRQRLA
                     ESFETALRHAEGRAIALEMDSDTEHLFSAKFACPVCSYSLQELEPRLFSFNNPVGACP
                     RCDGLGVVQFFDPKRIVAHPELSLAAGAIRGWDRRNQFYFQMLASLAKHYQFDIDTPF
                     ISLADGAQKVILYGSEREKLPFQYLNERGKIVLREHAFEGIVPSFERRYKETDSLAVR
                     EELAKYISNSTCPDCEGARLRKEARHVRIGSETLHAISRMPLADAQTFFRKQRLKGHK
                     AKVAEKIVKEIDSRLQFLINVGLDYLSLDRSAETLSGGEAQRIRLASQIGSGLTGVMY
                     VLDEPSIGLHQRDNARLLKTLAHLRDLGNTVIVVEHDEDAIRSADYVVDMGPGAGVHG
                     GRVVAQGKPEDILASTESLTGAFLGGRRQIAIPATRRVPTRGALRIVGASGNNLKQVD
                     AEIPAGLFTCVTGVSGSGKSTLINDTLYAAAAKHLYGSSLEPAPHDAIEGLDAFDKVI
                     NVDQSPIGRTPRSNPATYTGLLTPIRELFSGVPESRARGYGPGRFSFNVQGGRCEACR
                     GDGLLRIEMHCLPDVYVTCEVCGGSRYNRETLEVRYKGKNIAEILGMTVGE"
     gene            2460..3674
                     /locus_tag="VIS73_06175"
     CDS             2460..3674
                     /locus_tag="VIS73_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5762774.1"
                     /translation="MSTSADAAAHAGSHALSPEERRAGIGLASIFALRMLGLFLVLPV
                     FTLYAADIPGGTDPAMVGVALGAYGLTQALLQIPFGTLSDRFGRKRVIVIGLLIYAFG
                     SIVCALAGSIEMLIAGRVLQGAGAISAAVTALAADLTRDASRTRVMAMIGSSIGLVFA
                     LSLVIAPPLAGAIGLTGIFWLTALLSLAGIVVLLRLVPAEPPRPSKPKRTRFAEVLAD
                     RDLLRLNIGVFALHLLQVALWVVVPSALFTAGLGKTEHWHVYLPALLLSFAVMVPAII
                     AAERRGKMPQIFRAAVVLLVVVQAGLLWAGEGVWALGLWITLFFVAFNVLEATLPSLI
                     SRMVPADAKGAALGVYNTTQSIGLFLGGAVGGLLAKGGGPAAVHGFGLAVAVLWLAVS
                     VGMRFPVPRQGR"
     gene            3995..4987
                     /locus_tag="VIS73_06180"
     CDS             3995..4987
                     /locus_tag="VIS73_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY5762775.1"
                     /translation="MESIGTWWMWAGFAVIVVVMLAIDLFVVGGGRKHRVSLREAATW
                     SGVWVGVSLLFALALWAYLDATAGRALANEKSLQFVTGYLIEKSLAIDNVFVWLMIFG
                     FFAVPLELQKRVLIYGVLGAIVMRTIMIFAGVWLIAKFHWILYVFGAFLLVTGIKMWW
                     FAEEKPDLAKNPLILWLKSHMRISEHLEGERFFVMREETVAGVKTAVRYATPLFLVLI
                     LVEVSDLIFAVDSIPAIFAITTDPFIVLTSNVFAILGLRAMYFLLADMADRFTLLKYG
                     LALVLVFIGTKMLLIDLVKIPVMVSLAVVVTIIATSIVLSLRRTSRRELQSENV"
     gene            4974..>5644
                     /locus_tag="VIS73_06185"
     CDS             4974..>5644
                     /locus_tag="VIS73_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEY5762776.1"
                     /translation="MKTSELAMAVPAATSADWRVRLGAWLEDGRTQRVLIALIVINAV
                     VLGLETSRSVMEAVGSLLVALDRAILAVFVVEIALRLAVHRLAFFRDPWSVFDFSVVA
                     IALIPASGPLAVLRALRVLRVLRLVTLVPSMKRVVGGLLAALPGLGSVVAIIGLIFYV
                     AAVVATGLFAQAFPQWFGTLGASAFTLFQVMTLESWAMGIVRPVMEVYPYAWAFFVPF
                     IMVTT"
BASE COUNT          811 a         1801 c         1884 g         1148 t
ORIGIN      
        1 ttcccccacg gtcatgccca ggatctcggc aatgttcttg cctttgtagc ggacctccaa
       61 tgtctcccgg ttgtagcggc tcccgccgca cacctcacag gtcacgtaga cgtcggggag
      121 acaatgcatt tcgatccgga ggaggccgtc gccgcggcac gcctcacagc gccccccttg
      181 gacgttgaac gagaagcgcc cgggcccgta gccgcgggcc cgcgactcgg gtacgcctga
      241 gaacagctcg cggatcggcg taagcagccc ggtgtaggtc gccggattcg agcgcggcgt
      301 gcggccgatc gggctttggt cgacgttgat caccttgtcg aaggcatcga gcccctcgat
      361 tgcatcgtgc ggagccggct cgaggctgga gccgtagagg tgcttggctg ccgccgcgta
      421 aagcgtgtcg ttgatcagcg ttgacttgcc agacccggag accccggtaa cgcaggtgaa
      481 aaggcccgca ggaatctcgg catcgacctg tttcaggtta ttgccgctgg cgccgacaat
      541 gcgcagcgcg ccacgggttg gtacgcgccg ggtcgcggga atcgcaatct gccgcctgcc
      601 gccgaggaaa gcgccggtca gcgattcggt cgaggcgagg atgtcttctg gctttccctg
      661 cgcgacgacg cggccgccgt gcacgccggc gcccggcccc atgtcgacca cgtagtcggc
      721 ggagcggatc gcatcctcgt cgtgctcgac gacgatcacc gtattgccga ggtcgcgaag
      781 gtgcgccagc gtcttcagca gccgcgcgtt gtcacgctgg tgcaggccga tcgagggctc
      841 gtcgagcacg tacatgacgc cggtcagccc cgagccgatc tgtgaggcga ggcggatgcg
      901 ttgcgcctca ccgcccgaga gcgtctccgc cgagcggtcg agtgacaggt agtcgagtcc
      961 gacgttgatc aggaattgca gccggctgtc gatctccttg actatctttt ccgcgacctt
     1021 ggccttatgg cccttcagtc gctgcttgcg gaagaaggtc tgcgcgtcgg cgagcggcat
     1081 gcggctgatt gcgtgcaatg tctcgctgcc gatccgcacg tgccgcgctt ccttgcgcag
     1141 ccgcgcgccc tcgcagtcgg ggcaggtcga gttgctgatg tacttggcga gttcctcgcg
     1201 cacggcgagc gagtcggttt ccttgtagcg gcgctcgaaa ctcgggacga tgccttcgaa
     1261 ggcgtgctcg cgcagcacga tcttgccgcg ctcgttgagg tactggaagg gcagcttctc
     1321 gcgttcggag ccgtagagga tgactttctg ggcgccgtcg gcaaggctga tgaagggcgt
     1381 atcgatgtcg aactgatagt gcttggccag cgaggccagc atctggaagt agaactggtt
     1441 gcgccggtcc cagccgcgga tcgcgccggc cgcgagcgac agctcgggat gcgcgacgat
     1501 gcgcttgggg tcgaagaact gcacgacgcc aagcccgtcg cagcgcgggc aggcgccgac
     1561 cggattgttg aaggagaaca gccgcggctc gagttcctgc agcgagtacg agcagaccgg
     1621 gcaggcgaac ttggccgaga acaggtgctc ggtgtcgctg tccatttcca gcgcgatggc
     1681 gcgcccctcg gcgtgacgca gcgcggtctc gaaggactcg gcgaggcgct ggcgcatgtc
     1741 ctcgcgaacc ttgagccggt cgacgacgac atcgaccgtg tgcttcttcg acttggcgag
     1801 cttgggcagc gcgtcgatct cgtggatttc gccgttgatg cgcaggcgga cgaagccctg
     1861 cgcgcgcagt tcggcgaaca ggtcgagttg ctcgcccttg cggttggcga ccaccggcgc
     1921 gaggatcgac agacgtgttt cggccggaag ctccagcacg tggtcgacca tctgcgaaac
     1981 agtctgcgcc tcgagcggct cgccgtgctc ggggcagtag ggtgtgccgg cgcgggcaaa
     2041 cagcaggcgc aggtagtcat ggatttcggt gaccgtgccg acggtcgagc gcgggttgtg
     2101 actggtcgct ttctgctcga tcgaaatcgc cggactcagg ccttcgatca gatcgacgtc
     2161 gggcttttcc atcagctgca ggaactgccg tgcgtaggcc gacagcgatt cgacgtagcg
     2221 gcgctgcccc tcggcgtaaa gcgtgtcgaa ggccagcgag ctcttgcccg aacccgagag
     2281 gccggtgatg acgatcagcc ggttgcgcgg caggtcgagg tcgatgttct gcaggttgtg
     2341 ggtgcgggca ccgcggatgc ggaggtcttc catgctgggc tggggccggt tgggggcgaa
     2401 aatggcagcc tgctaatata cgggactccc cgattcgcct ccaatcccag agttcagcca
     2461 tgtcgacttc cgccgacgct gccgcccacg ccggttcgca cgccctgtcg ccggaagaac
     2521 gccgcgccgg catcggcctc gcgtcaatct tcgcgctgcg catgctcggg ctcttcctcg
     2581 tgctgccggt gttcacgctc tacgccgccg atattcccgg cggcaccgac ccggcgatgg
     2641 tcggcgtcgc gctcggtgcc tacggcctga cccaggcgct gctgcagatc cccttcggca
     2701 cgctgtccga ccgatttggc cgcaagcgcg tgatcgtcat cggcctgttg atctacgcgt
     2761 tcggcagcat cgtctgcgcg ctcgccggca gtatcgagat gctgatcgcc ggccgcgtgc
     2821 tgcagggtgc cggtgcgatt tcggcggcgg tgaccgcgct ggccgccgac ctgacgcgcg
     2881 acgcctcgcg cacccgggtg atggcgatga tcggttcgag catcggcttg gtcttcgcgc
     2941 tgtcgctggt gatcgccccg ccactggccg gcgccatcgg cctcacgggt atcttctggc
     3001 tgacggcgct gctttcgctt gccggcatcg tcgttctgct gcgcctagtg ccggccgagc
     3061 cgccgcgccc gagtaagccg aagcgcacgc gcttcgccga ggtgctcgcc gaccgcgacc
     3121 tgctgcggct gaacatcggc gtctttgcgc tgcatctgct acaggtggcg ctgtgggtgg
     3181 tcgtgccctc ggcgctgttc actgccgggc ttggcaagac cgagcactgg cacgtctacc
     3241 tgccggcact gctgctctcc tttgccgtca tggtgccggc gatcatcgcc gccgaacggc
     3301 gcggcaagat gccgcagata ttccgtgccg ccgtggtcct gctggtcgtc gtgcaggctg
     3361 ggctgctgtg ggccggcgag ggcgtctggg cgctcggcct gtggatcacg ctgttcttcg
     3421 tcgccttcaa cgtgctcgag gcgacgctgc cctcgctgat ttcgcgcatg gtgccggccg
     3481 acgccaaggg cgccgcgctc ggcgtctaca acaccacgca gtcgatcggc ctgttcctcg
     3541 gtggtgccgt cggcggccta ctggccaagg gcggtggtcc cgccgctgtg cacggcttcg
     3601 gcttggcagt cgccgtactg tggctggccg ttagcgtcgg catgcgcttc ccggtaccgc
     3661 ggcagggccg ctgaaccggc tcgccactcc gtggtgtcca ttgccctgga caagccccgg
     3721 aaattaagtt gactcttttg atcgggtaat ctatatttca tgcgtcccaa gggggagtag
     3781 ctctccgccg gcggcgtcgt caatacggaa accgtgatcc ggtttcccgg cgctccgggc
     3841 agtagtcagc acatcgactg ctgcgagcaa gacctttgcc gcgatggcag aggtgcccca
     3901 tgcagattca ggcccgctgt ccgtgctgga catcgggccg tttcgttttt gcctctcggc
     3961 agcgaacgga ttgcgaatcg aatgggagcg ttccatggaa tcgatcggca cctggtggat
     4021 gtgggccggt tttgcggtga tcgtcgtcgt aatgctggcg atcgacctgt ttgtcgtcgg
     4081 cggtggccgg aaacaccgcg tcagcctgcg cgaggcagcg acctggtcgg gcgtttgggt
     4141 tggcgtgtcg ctgctgtttg cactggctct ctgggcttac ctcgatgcga cggccggccg
     4201 ggcactcgcc aacgagaaga gcctacagtt cgtgaccggc tacctgatcg aaaaatcact
     4261 ggcgatcgac aacgttttcg tctggctgat gatcttcggc ttcttcgccg tgccgctgga
     4321 actgcagaag cgagtcctca tctacggcgt gctcggcgcc atcgtgatgc gcacgatcat
     4381 gatctttgcc ggcgtctggc tgatcgccaa gtttcactgg attctctatg tcttcggcgc
     4441 cttcctgctg gtcaccggca tcaagatgtg gtggttcgcc gaggaaaagc ctgaccttgc
     4501 gaagaatcca ctgatcctct ggctgaaaag ccacatgcgg atcagcgagc atctcgaggg
     4561 cgagcgcttc ttcgtcatgc gcgaggaaac cgtcgccggg gtgaagaccg cggttcgcta
     4621 cgcgacaccg ctctttctgg tgctgatcct cgtcgaggtc tccgatctga tcttcgccgt
     4681 cgattcgatt ccggcgatct tcgcgatcac caccgacccc ttcatcgtgc tgacctcgaa
     4741 cgttttcgcc atcctcggcc tgcgcgcgat gtacttcctg ctggccgaca tggccgaccg
     4801 cttcacgctg ctcaagtacg ggctggccct tgtattggtg ttcatcggca ccaagatgct
     4861 gctcatcgat ctggtgaaga tcccggtgat ggtctcgctc gccgtcgtcg tgaccatcat
     4921 cgccacctcg attgtcctgt ccttgcgccg tacttcccgg agggaactgc aaagtgaaaa
     4981 cgtctgaact cgcgatggcc gtaccggccg ccaccagcgc cgactggcgg gtccgcctcg
     5041 gcgcctggct ggaagacggc cggacgcagc gcgtgctgat cgcgctgata gtaatcaacg
     5101 ccgtcgtgct cggcctcgaa accagccgca gcgtgatgga ggcggtgggt tcgttattgg
     5161 tcgcgctcga ccgcgcgatt ctggccgtgt ttgtcgtcga gatcgcgctg cgcctcgccg
     5221 tgcatcggct ggccttcttc cgtgacccat ggagcgtctt cgacttcagc gtcgtcgcca
     5281 tcgcgctgat tcccgcctcg ggtccgctgg cggtgctgcg tgcgctgcgc gttctgcgcg
     5341 tgctgcgtct agtgacgctg gtgccgagca tgaaacgcgt ggtcggcggc ctgctcgcgg
     5401 cgctgccggg tctgggctcg gtggtcgcca tcatcggcct gatcttctat gtcgcggcgg
     5461 tggtcgccac cggcctgttc gcccaggcat tcccgcagtg gttcggcacg ctcggcgcga
     5521 gcgccttcac gctgttccag gtgatgacac tggaaagctg ggcgatgggc atcgttcgcc
     5581 cggtgatgga ggtctatccc tacgcctggg ccttcttcgt gcccttcatc atggtgacga
     5641 cctt
//
LOCUS       DATDAA010000188         2772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_14115,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000188 DATDAA010000000
VERSION     DATDAA010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14115"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..543)
                     /locus_tag="VIS73_06190"
     CDS             complement(289..543)
                     /locus_tag="VIS73_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY5762777.1"
                     /translation="MKITLKLYASLSDYLPTEARKLNAMELELAAGTTIAQVIERQNL
                     PPKSCHLVLIDGVFVPPAERAARPLAEGETLAIWPPIAGG"
     gene            complement(540..1787)
                     /locus_tag="VIS73_06195"
     CDS             complement(540..1787)
                     /locus_tag="VIS73_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5762778.1"
                     /translation="MKHVIIGNGPAGVIAAETLRRADPAADVVLLGDESGPPYSRMAI
                     PYLLTGKIDEAGTQLRKDAGHYKRLGIRLMKGRAKKVDSKTRRVTLANGKKLPYDRLL
                     IASGAHAVRPDVPGIESPNVHPCWTLADARAIAKKAKPGARVVQLGAGFIGSIILQAL
                     AERGVKLTVVEMGDRMVPRMMTAKAGNLIRLWVEKKGVQVHVNARVESIAKAARGAMK
                     VTLSNGSMLDCNLLICAAGVRPNVDFLEGSGVRIGDGIMVDERMQTSVPEIYAAGDVT
                     EAAGFHSGRPELNAIQPNAADQARIAAANMAGKHAAAQGSLAINVLDTLGLISSSFGQ
                     WQGVAGGDGVELVDEPGFRYLSLQFKDDMLIGATSIGWTEHVGALRGLIQGRVRLGGW
                     KARLLQDPTQFMAAYLGSAQKAA"
     gene            complement(1903..>2772)
                     /locus_tag="VIS73_06200"
     CDS             complement(1903..>2772)
                     /locus_tag="VIS73_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5762779.1"
                     /translation="GLVFEAAWALGAANGVGDLEALQYANLLCNEDGYDPISFGATVG
                     AAMELYELGILKKEKIGIEAPFGSAQALVQLSEMTAKGEGFGKELGLGSARLCAKYGK
                     PELSMGVKKQEFPAYDARGIQGMGLAYATSNRGACHLRGYTVASEVMGVPVKTDPLVT
                     EGKADLAKAFQDATAVFDSAGICIFTSFAWTLAEVQPQVDAACEGGWSMEKLATMGER
                     IWNMERLFNNAAGFTAKDDDLPPRLKTEPAKTGPAKGKVNEIDKMRPEYYKARGWDEK
                     GVPTKETLKRLGL"
BASE COUNT          432 a          930 c          865 g          545 t
ORIGIN      
        1 ccatcaacac ttgaaccgcc gagatgccgc gggctcggca catgaaccaa tcgcgctaac
       61 cggcggtgga ttgacctaag cttaaaacag aaaacgggtt gtcgtttttt gtatcggcgt
      121 ttatcggcgt ttatcggcgg ccaataaacg atgctccgag ctctgggttc agggtttcct
      181 gtatatgcaa tcctgtcttt atctgcgtgt atctgcgttc atctgcggtt aaacacgttt
      241 tgcgtctcgc gttttacgtt ccactgctgc gcccatggcg gctcgctgtc agccgccggc
      301 aatcggcggc cagatcgcca gcgtctcgcc ctcggcgagc gggcgtgctg cgcgctccgc
      361 gggcggcacg aacacgccgt caatcagcac cagatggcag gacttgggcg gcaggttctg
      421 gcgctcgatg acctgggcta tggtcgtgcc ggcagcgagc tcgagctcca tggcgtttaa
      481 cttgcgcgcc tcggtcggca gataatccga aagcgaggcg taaagcttga gcgtgatttt
      541 catgcagctt tctgggccga acccaggtat gccgccatga actgcgtcgg atcctgcagc
      601 agccgcgcct tccagccgcc aagccgcaca cggccctgga tgaggccgcg cagcgcgccg
      661 acatgctcgg tccagccgat gctggtggcg ccgatcaaca tgtcatcctt gaactgcagc
      721 gacaggtagc ggaagcccgg ctcatcgacg agctcgacgc cgtcgccgcc cgccacgcct
      781 tgccactggc cgaacgagct cgaaatcagt cccagggtat cgagcacgtt gatggcgagg
      841 ctgccttgcg cggcggcgtg cttgcccgcc atgttggcgg cggcaatgcg cgcctggtcc
      901 gccgcgttgg gctggatcgc gttgagctcg ggccggccgg aatgaaaccc ggcggcctcg
      961 gtgacgtcgc cggccgcgta gatttccggc acgctggtct gcatgcgctc atcgaccatg
     1021 atgccatcgc cgatcctgac gccgctgccc tcgagaaagt cgacattcgg acgcacgccg
     1081 gccgcgcaga ttagcagatt gcaatcgagc atgctgccgt tggaaagcgt gactttcatc
     1141 gcgcccctgg cggcctttgc gatcgactcg acgcgcgcat tgacgtgcac ctgcacgccc
     1201 ttcttctcga cccacagccg gatcagattg cccgccttcg ccgtcatcat gcgcggcacc
     1261 atgcggtcgc ccatttccac cacggtcagc ttcacgccgc gttcggctag cgcctgcagg
     1321 atgatgctgc cgatgaagcc cgcgccaagc tgcacgacgc gcgcgccggg cttggccttc
     1381 ttcgctatcg cccgcgcatc agcgagcgtc cagcacggat gcacgttggg agattcgatt
     1441 ccgggcacat cgggccgcac cgcgtgcgcg ccgcttgcga tcagcaggcg gtcgtacggg
     1501 agtttcttgc cgttggcgag cgtgacgcgg cgcgtcttgc tgtcgacctt tttggcgcgc
     1561 cccttcatca ggcggatgcc cagccgcttg tagtgccccg cgtccttgcg cagctgcgtt
     1621 cccgcctcgt cgatcttccc ggtgaggagg taggggatcg ccatgcgcga ataaggcggc
     1681 ccgctctcgt cgccgagcag gacgacatcg gcggcgggat cggcacggcg cagggtctcc
     1741 gcggcgatga cgccggccgg accgttgcca atgatgacgt gcttcatgaa ttgtccttcg
     1801 gaaaaagcgg acggtcgcat agcagccccg cgcctgctgc ctcaccctgc ccgctctccc
     1861 cctgcgcggg ggagagggac ttgcatttgc ctgggcgcgc ccttacagac cgagccgctt
     1921 cagcgtctcc ttggtgggca cgcctttttc atcccagccg cgcgccttgt agtactccgg
     1981 ccgcatcttg tcgatctcgt tgaccttgcc cttggccggg ccggtcttcg ctggctcggt
     2041 cttgagacgc ggcggcaggt cgtcgtcctt tgcggtgaaa ccggctgcat tgttgaacag
     2101 gcgctccatg ttccagatac gctcgcccat cgtcgccagt ttctccatcg accatccgcc
     2161 ttcgcaggcg gcgtcgactt gcggctgcac ctcggccagc gtccaggcga aactcgtgaa
     2221 gatacagatg ccggcggaat cgaacacggc ggtcgcgtcc tggaacgctt tggccagatc
     2281 ggccttgcct tcggtcacca gcggatcggt cttgaccggg acgcccatca cctcggaggc
     2341 gacggtgtag ccgcgcagat ggcaggcgcc gcgattggac gtggcgtaag cgaggcccat
     2401 gccctggatg ccgcgcgcgt cgtaggccgg aaactcctgt ttcttgaccc ccatggagag
     2461 ctcgggcttg ccgtacttgg cgcagagccg ggccgagccc agccccagtt ccttgccgaa
     2521 gccctcgccc tttgccgtca tttccgagag ctgcaccagc gcctgggccg agccgaaagg
     2581 cgcctcgatg ccgattttct ccttcttcag gatgcccagc tcgtacagtt ccatggccgc
     2641 gcccacggtg gcgccgaagg aaatcgggtc gtagccgtcc tcgttgcaaa gcaggttggc
     2701 atactggagc gcctccaggt cgccaacgcc attggctgcg cccaatgccc atgcggcctc
     2761 gaatacgagg cc
//
LOCUS       DATDAA010000189         2455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_433715,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000189 DATDAA010000000
VERSION     DATDAA010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433715"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..1803)
                     /locus_tag="VIS73_06205"
     CDS             complement(295..1803)
                     /locus_tag="VIS73_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762780.1"
                     /translation="MESLLRKLSRGLGLGNAARPAASRLPPLLGRLAGLPPADVVRAM
                     TPEVHRCVTAETDFARRIGLIDALREAAETALPALEDEIAAVRLPLAVPQMAIAVTID
                     NLLKALAGEYQSVVVALAASSGRNSSVPLARAALHATRLLARRQWLATLAGKASSRTA
                     WRQFHRLHRLSREAGFSDFSEGDGSIDHIYRRAILLTLAEADRLPRADLASLRAALDV
                     LTPLVRLTDARATPASATEAVFISGRDPLPMSGDPRDVSLHPAWELHLDAVVEQLQNE
                     IRDAERAREAGVATLQAMQLRLLGRLLERFRGKPIRRFSRQGMRPRVDVVTGIDRLWW
                     ALSGPGLSRRRPGELSLAPQDGREWMIVDESPDGFGLKLVRGNCPEIEVGDIVGIGLR
                     DVGRMHVCIVRRVDEDPIQRRRIGVQTLAPDAAAIVVLATEPSRQEAVVHLARLPAHG
                     GRAGLACASGRLTPGTMVRIVDSEEGGALKIGAPIESNGRFQLLLLDPQPGT"
     gene            complement(1932..>2455)
                     /locus_tag="VIS73_06210"
     CDS             complement(1932..>2455)
                     /locus_tag="VIS73_06210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5762781.1"
                     /translation="FAGMFVYVLSAPVFLIEHLGLSAAQFGWLFVPLTAGMMLGAALS
                     GRLAGKLSPRRTVAVAYAAMTTAATINLMIGLSAPSSIAWYLLPLPLYTAGMSLAMPS
                     LTLIALDRFPARRGLAAAVQGLMNTGAAGLSAGLLVPLLWGSPLTLALGMALLLAAGA
                     IAFTLSVRTARIR"
BASE COUNT          417 a          785 c          874 g          379 t
ORIGIN      
        1 cgatggcgcg cgccagcggg tcgggcaagt catgcggttg agcacttcca agcggcctca
       61 taggcgactc cggggcgagc cgcatagacg acccggaaga ctgggctgcg ccatacggca
      121 gatgctaaac aaatgtgccg cggcctggcg ggcagcggac ctccgcgaga atatggacaa
      181 gttttgcgct tgccgcaagc cgttaagccc ccttatttct aggcgaagcc gcgaaactta
      241 gtcgcgccct ttactgatgg gatatggctt tagtattagt ctatgcactt agtattaggt
      301 gcccggctgc ggatcgagca gcagtaactg gaagcgcccg ttgctttcga ttggtgcgcc
      361 gatcttcagc gcaccgcctt cctcgctgtc gacgatccgc accatcgttc ccggggtgag
      421 gcggcccgaa gcgcaggcca aaccggcacg gccgccgtgc gccggcaggc gcgcgaggtg
      481 gaccaccgct tcctgacgtg acggttcggt ggcaagcacg acgattgccg cggcgtcggg
      541 cgccagcgtc tgcacgccga tccgccgacg ctggatcggg tcctcgtcga cgcggcgaac
      601 gatgcacacg tgcatgcgac cgacgtcgcg caggccgatg ccgacgatgt cgccgacctc
      661 gatctcgggg cagttgccgc gcaccagctt gaggccgaag ccgtccgggc tctcgtcgac
      721 gatcatccat tcgcggccat cctgcggtgc cagcgacagc tcgccgggac gccgacggct
      781 gaggccgggt cccgacagcg cccaccacag ccggtcgatg ccggtcacga catcgacgcg
      841 tgggcgcatg ccctgccgcg agaagcgacg gattggtttg ccacggaagc gttcgagcag
      901 gcgtccgagc aggcgcaact gcattgcctg cagggtggcg acgccggcct cgcgggcccg
      961 ttcggcatcg cggatttcgt tttgcagttg ttcgacgacg gcgtcgaggt gcagctccca
     1021 ggccgggtgc aggctgacgt cgcgcgggtc gccagacatc ggcaaggggt cgcggccgct
     1081 gatgaaaacc gcttcggtcg ccgatgctgg tgtcgcccgg gcgtcggtca ggcgaaccag
     1141 tggcgtcagc acgtcaagcg ctgcacgcag cgacgcgagg tcggcacgcg gtagacgatc
     1201 cgcttcggca agcgtcagca ggattgcccg ccggtagatg tggtcgatcg agccgtcgcc
     1261 ttcgctgaaa tcggagaagc ctgcttcgcg tgatagtcgg tgcaggcggt gaaactggcg
     1321 ccaggccgta cgcgacgacg ccttgccggc gagcgttgcc agccactgtc ggcgcgccag
     1381 caggcgtgtc gcgtgcagtg cggcgcgagc cagcggtacc gacgagttgc ggccggacga
     1441 tgcggcgagt gcgacgacga ccgactggta ttcgccggcg agtgccttga gcaggttgtc
     1501 gatggtgacc gcgatggcca tctgcggcac ggcaagcggc aggcgcaccg cggcgatctc
     1561 gtcctccagc gccggtagtg cagtctcggc ggcctcgcgc agcgcatcga tgaggccgat
     1621 gcggcgggcg aaatcggtct cggcggtgac gcagcgatgg acttccggcg tcatcgcgcg
     1681 cacgacgtcg gccggcggca gaccggcgag gcggccaagc agcggcggta gccgcgaggc
     1741 ggcggggcgt gcggcattgc ccagtcccag tccccgggac agcttgcgaa gcagggattc
     1801 catgcggtgg cgatcctggt cggccatggt cgatgcccgg caatgtagcc gctggtcaag
     1861 cccttgaaca gcaaggattt ggcgcttttg cccggaaaat ctccagggcg ctgccagtcg
     1921 caattcgggt ttcagcgaat tcgtgctgtt cgcaccgaga gcgtaaaggc gatcgcgccg
     1981 gcggccagca gcaacgccat gcccagcgct agcgtcagcg ggctgcccca cagcagaggc
     2041 accagcaggc ctgccgagag tcccgcggcg ccggtgttca tcaatccctg cacggcggcg
     2101 gcgagtccac gtcgcgcggg gaaacggtcg agcgcgatca gcgtcaggct gggcatcgcc
     2161 agcgacatgc ccgctgtgta caagggcagc ggcagtaggt accaggcgat gctcgatggt
     2221 gcgctgaggc cgatcatgag gttgatcgtc gccgccgtgg tcatggcggc gtaggccacc
     2281 gcaacggtgc gccgaggcga gagctttccg gcgagccgtc ccgacagagc ggcgccgagc
     2341 atcatgccgg cggtcagcgg cacgaacagc cagccgaatt gcgccgccga gagcccgaga
     2401 tgttcaatca ggaacaccgg cgccgagagc acgtagacga acatgccggc gaagt
//
LOCUS       DATDAA010000190         5671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_258904,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000190 DATDAA010000000
VERSION     DATDAA010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258904"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VIS73_06215"
     CDS             complement(<1..434)
                     /locus_tag="VIS73_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY5762782.1"
                     /translation="MKSILVWDWPLRVFHWTLATLVVVSIVSGQVGGNWIDWHSRSGI
                     AIVGLLAFRLAWGVIGSTHARFLSFVCGPRTILAYLRGEWRGIGHNPLGALSVLGLLS
                     VLAAQAGTGLFGNDDIAFNGPLYALVDKSTSDLAISWHKTIF"
     gene            599..1738
                     /locus_tag="VIS73_06220"
     CDS             599..1738
                     /locus_tag="VIS73_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY5762783.1"
                     /translation="MSETGTRTALVMSGGGARAAYQVGVLQAIRDVVGDGRQNPFQII
                     CGTSAGAINAVALACGAQNFRAAVDDLADVWWNFRTNQVYRSDIGGMSLTSVRWLGAL
                     ALGWVFRSNPKYLLDNRPLAELLRQRLDFSRIDQAIATGALYAISVTASGYTSGQSVS
                     FFQAQAEVKPWTRAQRIGSRVRLGIDHLLASSALPFIFPAMRIHREFFGDGSMRQLAP
                     ISPAIHMGADRVFVIGSSHLQEPDVRLVQSTYPSLAQIAGHVMSAIFLDSLAADIERL
                     ERINQTLSRIPEDLRVENGIRLRPIRALIIQPSERIDRLAARHGRVLPWPVRTLLRGV
                     GAMHRRGGALASYLLFEQPFTRALIEMGYRDTMARRDEVEAFFGP"
     gene            1900..2823
                     /gene="pbpG"
                     /locus_tag="VIS73_06225"
     CDS             1900..2823
                     /gene="pbpG"
                     /locus_tag="VIS73_06225"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEY5762784.1"
                     /translation="MAALVSAVFGLTLWPSAESFAAESAKVRKAASSKAGKSRHASAR
                     RSAKPVPAMPKLRSGSAAVVDLESGRILFEKNADEVSPIASITKLMTAMVVLDSAASL
                     GDELEVTRDDVDREKYSRSRLPVGSRLTREDALLVALMASENRAASVLARNYPGGTEA
                     FVAAMNAKAIELGLHNTHFDDPTGLSSANVSTASELVSLVSAAHRYPLIREFSTTQRT
                     TLQLGKRRVSFGNTNALVKNRYWEISLSKTGYIREAGKCLVMQAWVDGRPVAVILLDS
                     YGRLTRLADAKRIRDWMRRETIARGAGDLTS"
     gene            complement(2830..3618)
                     /locus_tag="VIS73_06230"
     CDS             complement(2830..3618)
                     /locus_tag="VIS73_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY5762785.1"
                     /translation="MPPKSKTEGDESKGSIQVIDRMMALLEVLASSPDPVALKTLAAS
                     TKLHPSTAHRILASMSQGRIVERQGAGSYRLGIRLLELGNLVKTRINLREVALPFMQT
                     LHEHIGEAINLAIRRDDDLVYIERTSSGRSLVRVVYLVGDRAPLHLTSVGKLFLAEDN
                     AEALRQYAKRTGLPGKTPHSLTTLTALERDLERVRRHQLAYDNEEAEVGLKCVAAPIR
                     DDEGNLIAGLSVSAPTERHHADWTAKVATTANDISRALGYATQR"
     gene            3778..4461
                     /locus_tag="VIS73_06235"
     CDS             3778..4461
                     /locus_tag="VIS73_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5762786.1"
                     /translation="MSKRPNPYGPEVEPEADGSPAIPLWIGGHAFLTMPDAGFFDIRD
                     GEGRVLRRVPLCGADAVDAAVAAAIAAAATPWLAEDWLAIRAEIGALAGRYAQHLEGL
                     LHSENGDGFDARAELLPLTEGPAGSAAVTDRCAPQIVALVGLASEPLAGLASAALDLL
                     AAGHAVILKPSVKTPSAVLAFAELATRAGLPGGRLNVVHGDEAVVQALCAHPGISRVV
                     CIGTGETAE"
     gene            complement(4539..>5671)
                     /locus_tag="VIS73_06240"
     CDS             complement(4539..>5671)
                     /locus_tag="VIS73_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY5762787.1"
                     /translation="LILAGFLLNAGAPPLSAWLPDAYPEASPSGMVTLSAFTTKTAVY
                     VLLRGFPGTEILIWLGIFMIFYGIVYALLENDMRRILAYSIVNQVGFMVTGIGIGTEM
                     ALNGAAAHAFTHIIYKALLLMSAGSVLLMTGRRKCSELGGLFHTMPIATICGTIGALA
                     ISSFPFTSGFVSKSMVTQAAVDGHLLWIWLALAAASAGVFLHAGIKFPWFVFFQKDSG
                     LRPPDPPASMRWAMIFFAFLCIALGVWPEPLYAMLPYAVNYVPYTAAHVLTQLQLLLF
                     SGLAFFVMLKYLKRTPTITLDTDWTYRVLFANLALLLRDALRAMFAAADQVRTAIARE
                     FGAFIVRETHHAGLFVPSRSTRGMATWVMTLLLIYLAAYYIV"
BASE COUNT         1032 a         1750 c         1865 g         1024 t
ORIGIN      
        1 cagaagatgg tcttgtgcca gctgatcgcg agatcactgg tcgacttgtc gaccagtgcg
       61 tagagagggc cattgaaggc gatatcgtcg ttgccaaaca ggccggtccc ggcctgtgct
      121 gcaagcaccg aaagtaggcc cagcaccgac aacgcgccga gcgggttgtg gccgatgccg
      181 cgccattcac cgcgcaggta ggcgaggatc gtccgcggac cgcagacgaa actgaggaag
      241 cgcgcatggg tcgagccgat gacgccccag gcgagtcgaa aggcgagcaa gccgacgatc
      301 gcgatgcctg aacggctatg ccagtcgatc cagttgccgc cgacctgtcc gctgacgatg
      361 gagacgacaa ccagcgtcgc gagggtccag tggaaaaccc gcaggggcca gtcccagacc
      421 agtatcgact tcatctgcct ctccattccg acgacattgc ggcccggata ttagcatcgc
      481 gccgaggtgg tgatttaatc ccgttccgcc tcgctgcgcg gcattgccgc gacggccggg
      541 caagccgtat cctttccccc atgtcgaacc gacggaactc cattcaggtc agtcagcgat
      601 gagcgaaacg ggtacgcgaa cggcgctggt gatgtcgggt ggtggcgcgc gcgcggccta
      661 tcaggtcggc gtattgcagg caattcgcga tgtggttggc gatgggcgac agaatccgtt
      721 tcagatcatc tgcggcacat ctgccggggc gatcaacgcg gtggcgcttg cctgcggcgc
      781 gcagaacttc cgggcggccg tcgatgacct tgccgacgtc tggtggaatt tccgcaccaa
      841 tcaggtttat cggtccgaca tcggtggcat gtcgctgacc agcgtgcgtt ggctcggcgc
      901 cttggccctg ggctgggtct ttcgctcgaa tccgaaatac ctgctcgaca accgaccgct
      961 tgccgaactg ctccgccagc ggctcgattt ttcgcggatc gaccaagcga tcgccaccgg
     1021 cgcgctgtac gcgatcagcg tcacggcatc gggctatacc tcggggcaga gcgtcagctt
     1081 cttccaggcg caggccgagg tgaagccatg gacgcgcgcc cagcgaatcg gatctcgcgt
     1141 ccgcctcggg atcgaccacc tgctggcatc gagcgcgctg ccattcatct tcccggcgat
     1201 gcgcatccat cgcgagtttt ttggcgacgg gtcgatgcgc cagcttgcgc cgatttcccc
     1261 ggcgatccac atgggggccg accgcgtctt cgtgatcggc tcctcgcacc tccaggagcc
     1321 cgatgtccgg ctggtccaga gcacctaccc gtcgctggcg cagatcgccg gacacgtcat
     1381 gtcggcgatc tttctcgatt cactggcggc cgatatagag cggcttgaac ggatcaacca
     1441 gaccctgagc cgaattccgg aggaccttcg cgttgaaaac ggcattcggc ttcgcccgat
     1501 ccgcgcgttg atcattcaac cgagcgaacg gattgatcga ctggcggcgc gccacgggcg
     1561 cgtcttgccg tggccggtgc gcacgttgct caggggcgtt ggcgcgatgc atcgtcgggg
     1621 cggggcgctg gcgagctacc tgctgttcga gcagccgttt acccgggcgc tgatcgagat
     1681 gggctaccgc gacacgatgg cccgtcgcga cgaagtcgaa gccttcttcg gcccttaagt
     1741 gtggcaaact tcagacgtta tctgagatag cctctgcatt cctttgttta tttgtgattt
     1801 gtcgaggcga gcggacgccg ctagaatggc ggcccattga ggagcgctgg agtcgggatg
     1861 cgggcaggga caagacgacg cgggttcggt acctggggca tggctgccct ggtctcggcg
     1921 gtattcggtc tgacgctttg gccgtctgcc gaaagcttcg ccgctgagtc ggcgaaggtc
     1981 cgcaaggccg cttcaagcaa ggccggaaag tcccgccatg cgagcgcgcg gcgcagcgcc
     2041 aagccggttc cggcgatgcc gaaactgcgt tcgggttcgg ccgccgtcgt tgacctggaa
     2101 tccgggcgaa tcctgttcga gaaaaacgca gacgaagtct cgccgatcgc ctcgatcacc
     2161 aagctgatga ccgcgatggt cgtcctcgat tcggctgcct cgctcggcga cgagcttgag
     2221 gtcacacgcg atgacgtcga ccgcgaaaaa tattcacgct cgcgcctgcc cgtcggtagc
     2281 cgcttgaccc gagaagatgc cctgctggtt gccttgatgg cctcggaaaa tcgcgctgcc
     2341 tcggtgctcg ctcgcaatta ccccggcgga accgaggcct tcgtcgccgc gatgaatgcc
     2401 aaggccatcg aactggggct tcataacacg cactttgacg acccgaccgg gctgtcgagt
     2461 gccaacgtat cgacggcgag cgagctggtc agcttggtca gcgcggccca tcgataccca
     2521 ctgattcgcg agttttcgac cacacagcgc acaaccctgc aacttggcaa acgtcgggtg
     2581 agtttcggca acaccaatgc gctggtcaag aaccgctatt gggaaatcag cctgtcgaaa
     2641 accggataca tccgcgaggc cggcaagtgc ctggtgatgc aggcctgggt tgatggacgg
     2701 ccggtggcag tgattctgct cgattcctac ggtcggctga cccggctggc tgacgccaag
     2761 cgcatccgcg actggatgcg gcgcgaaacc atcgcccgcg gcgctggcga cctcacttcc
     2821 tgatccgact caacgctggg tcgcgtagcc cagcgcgcgc gagatatcgt tggccgtagt
     2881 ggcgaccttg gctgtccaat cggcgtgatg tctctcggtc ggggccgaca ccgatagccc
     2941 ggcaatgaga ttgccctcgt cgtcacgaat cggcgcagcg acgcatttca gccccacttc
     3001 cgcttcttcg ttgtcgtagg ccagctggtg ccggcgaacg cgctcgagat cgcgttcgag
     3061 cgcggtcagt gtcgtcagcg agtggggtgt cttgcccggc aagccggtcc gcttggcgta
     3121 ttggcgaagc gcctcggcgt tgtcttcggc gagaaacagc ttacccactg aggtcaggtg
     3181 caagggcgcg cgatcaccga ccagatagac gacgcgcacc agcgagcggc cgctcgaggt
     3241 ccgctcgatg tagaccagat cgtcgtcgcg gcggatcgcc agattgatcg cctcaccaat
     3301 gtgttcgtgc agcgtttgca taaagggcag cgcgacctcg cgcaggttga tccgcgtctt
     3361 gaccaggttt cccagttcga gcaggcggat gccgaggcgg tagctgcccg ctccctggcg
     3421 ttcgacgata cggccctgcg acatcgaagc gaggatacgg tgtgcggtcg agggatggag
     3481 cttcgtggac gcggcgagcg tcttcagggc aaccggatca ggcgatgagg caaggacttc
     3541 aagcagtgcc atcatccggt cgatgacctg gatcgatccc ttggattcgt cgccttcagt
     3601 ctttgatttc gggggcatag gatttaccat tggccgctcc gcgggggcca agcggcgcga
     3661 tcgttcgcca ttccgcgaac gccgtgtgtc gatgcagtat atcgcatggt gaattcccat
     3721 tttgaattgt gtagccgttc gttgccgcaa cttccctcga gatcaagaga aatgaaaatg
     3781 agcaaacgcc caaatcccta tggaccggag gtcgagccgg aggccgatgg atcgccggcg
     3841 attccgctgt ggattggcgg ccacgcattc ctgaccatgc ccgacgccgg cttcttcgat
     3901 atccgcgatg gcgagggccg ggttcttcgc cgggtcccgc tatgcggcgc cgatgcggtt
     3961 gatgctgccg ttgccgccgc cattgcggcc gctgcgacgc cctggctggc cgaagactgg
     4021 ctggcgatac gcgctgagat tggcgccttg gcaggacgtt acgcccagca cctcgagggt
     4081 ttgctgcact ccgaaaacgg cgatggcttc gacgcccgcg ccgaactgtt gccgcttacc
     4141 gaaggccccg cgggcagtgc cgccgtcacc gatcgctgcg cgccgcaaat cgtcgcgctg
     4201 gtcggcctcg ccagtgaacc gcttgccggc ctggccagtg ccgcactcga tctgctggcg
     4261 gcaggccatg cggtgatcct caagccctcg gtgaagacac cctcggcggt attggccttc
     4321 gccgagctcg cgacgcgcgc cggattgccc ggcggccggc tcaacgtcgt tcatggtgat
     4381 gaggccgttg tccaagcgct gtgcgcgcac cccggcattt cgcgcgtcgt ctgcatcggt
     4441 accggtgaga cggccgagtg aattcgcgca atgctcgctg cggcggtcag gccaggcggt
     4501 gcttcagggg actgaatcgg cggcgctgcg gcttggtgtc agacgatgta gtaggcggca
     4561 aggtagatca gcagcagcgt cattacccag gtcgccatgc cgcgggtcga ccggcttggt
     4621 acgaacaggc cagcatggtg ggtctcgcgg acgatgaagg cgccgaactc gcgggcaatc
     4681 gcggtgcgaa cctgatcggc agcggcgaac atggcgcgca gcgcatcccg cagcagcagc
     4741 gccaggttgg cgaacagcac acggtaggtc cagtcggtat cgagcgtgat tgttggcgtc
     4801 cgcttcaggt acttgagcat cacgaagaag gccagacccg agaacagcaa gagctgcaac
     4861 tgcgtcagca cgtgcgccgc cgtgtacggc acgtaattga ccgcataggg cagcattgcg
     4921 tacagcggct cgggccagac gccgagcgcg atgcagagga aggcgaagaa gatcatcgcc
     4981 cagcgcatgc tcgccggcgg atcgggcgga cgcagccctg agtccttctg gaaaaacacg
     5041 aaccacggga acttgatgcc ggcatggagg aagacaccgg ccgatgccgc ggccagtgcc
     5101 agccagatcc acagcaggtg tccgtcgacc gccgcttgcg tcaccatcga cttggagacg
     5161 aatcccgagg tgaacgggaa cgaggagatc gccagcgcgc cgattgtgcc gcagatcgtc
     5221 gcgatcggca tcgtatggaa aaggccgccg agttccgagc acttgcgacg cccggtcatc
     5281 agcagaaccg agccggccga catcagcagc agcgccttgt agatgatgtg cgtgaaggcg
     5341 tgggcggcgg cgccgttaag cgccatctcg gtgccgatgc cgatgccggt aaccatgaag
     5401 ccaacctggt tcacgatcga ataggcgagg atgcgccgca tgtcgttctc gagcagcgcg
     5461 tagacgatgc cgtagaagat catgaagatg cccagccaga tcaggatctc ggtgccggga
     5521 aagccgcgca gcagcacgta gacggcggtc ttggtggtga aagcggacag cgtgaccatg
     5581 ccgctgggtg aagcctcggg gtaagcgtcg ggcagccacg ccgacagcgg cggcgctccg
     5641 gcgttgagca gaaaaccggc aagaatcagc c
//
LOCUS       DATDAA010000191         5993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_14216,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000191 DATDAA010000000
VERSION     DATDAA010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14216"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="VIS73_06245"
     CDS             <1..1027
                     /locus_tag="VIS73_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY5762788.1"
                     /translation="MILDDGGDATLLLHLGARAEKDPSVLGKPASEEERVLFAAIKKR
                     NAEQPGWYAKNLEAIRGVTEETTTGVHRLYQMHQRGELKFPAINVNDSVTKSKFDNLY
                     GCRESLVDGIKRATAVMIAGKVALVAGYGDVGKGSAQALRALSAQVWVTEIDPICALQ
                     AAMEGYRVVTMDYAADKADIFVTATGNYHVIGHEHMAKMKDQAIVCNIGHFDNEIDVA
                     SIEGYPWEEIKPQVDHITMPSGNKIILLAKGRLVNLGCATGHPSYVMSSSFANQTIAQ
                     IELWTKRDSGDYPVGVYTLPKHLDEKVARLQLKKLNSQLTTLTKEQADYIGVPVEGPY
                     KSDHYRY"
     gene            1140..1802
                     /locus_tag="VIS73_06250"
     CDS             1140..1802
                     /locus_tag="VIS73_06250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5762789.1"
                     /translation="MSRKKPTIRHTRESDVDPLIALQKETYPTIPPWRRERLLNQLEV
                     FPQGQIVAELNGEIVGAASSLVVLWDEWLTDHNWSEITASGSFNTHNPDGRTLYGAEV
                     FVHPYLRGAGIGHALYKGRRKLCRAMNLKRIIACGRLPGYHLHAENMSAELYAQKVVW
                     GDLRDPVLSFQLKEGFSYCGTVDDYIPEDKESLGHASLIVWLNMRYNAKKPTKIPQGD
                     LL"
     gene            1799..2671
                     /locus_tag="VIS73_06255"
     CDS             1799..2671
                     /locus_tag="VIS73_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY5762790.1"
                     /translation="MKVRIAAVQYLLRAIADWDGFENQVRFVMKACGDYRPHFVMLPE
                     IFTSQLLSFLDDGDIKGAVRRMNDFTPRYIDLMTELSKKYGFYLIAGSHPNLRDDQLY
                     NTAFMFSPRGDVYTQDKIHRTRWEREKWDTAAGDKLSLFQTEYGAIAILICYDIEFPE
                     LARMVCEAGADILFVPSSTDDRQGFWRVRYCAQARAIENQVYVAMTSTVGNLPVEGLG
                     LHYGQASILTPSDFPFARDGIAAEGVVNMEQIIIADVDLDALMLNRSNGTTIPLYDKR
                     REVYDNPVETISTR"
     gene            2691..3539
                     /gene="metF"
                     /locus_tag="VIS73_06260"
     CDS             2691..3539
                     /gene="metF"
                     /locus_tag="VIS73_06260"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEY5762791.1"
                     /translation="MPNRLVPISCEFFPPQTDEGIAKLRATREALTALAPEFFSVTYG
                     AGGSTRERTFEAVFDIHAAGQTVAPHLSGIGSSRATIREILAAYRDRGIRRLVALRGD
                     LPSGMAASGEFRYASELVEFIRAETGDWFTIEVAAYPEWHPQARTPADDLKAFAAKVA
                     AGADSAITQYFYNADAYFRFVDDARTAGIRVPIVPGIMPITSFSKLARFSDACGAEIP
                     RWLRWKLEGFGDDSASIRALGLDVVTALCERLLAGGAPGIHFYTMNQAAATTAICTRL
                     RLTPAP"
     gene            complement(3523..5409)
                     /locus_tag="VIS73_06265"
     CDS             complement(3523..5409)
                     /locus_tag="VIS73_06265"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY5762792.1"
                     /translation="MRPLGIRGRITLLAVLPVLFTSIAIGGILVRERLKADQAQMVYV
                     GGLLARQLASNAEFGLFSGNRDLLESIASSALTERAVVLVSIRDGRGDALVSLGDQTR
                     VRRAVPLTGFAEGERWETDSVMIGRLVRRSASDVFDELFASSPVDESPPIGAVEVVLS
                     LEPLRRDARGFMLWSATIAATVVAVVTVLSLRFGERLVGPLRRLGMAIERIGGGDFGA
                     RLPPEGGGDMRRLANGVNRMAERLGASRTELERRVEEATAELSEKKREAELANEAKSR
                     FLAAASHDLRQPMHALTLFADQLNRQPLKGEAIVLAHRIQEASESLAGLLDSLLDISR
                     LDAGAFVPNRRVFALAPLLSRLQAEYGESARAAGLRLRVRPSALSVESDPVMCERVLM
                     NLISNAIRYTERGSILVAARLRSGMVRIEVRDSGTGIPEALQALVFSEFVQLANPERD
                     QRKGLGLGLAIVARLCALLGHRLGLSSALGRGSCFWFELPPARALPTDAQEADLSSLA
                     GRRVGIVEDDVAIVAGMRDLLASWGCSSVGAASLEELGANLAGADAKLDALICDHTGG
                     EFGPQTAAEIATRLGYSVPLVIVSADSGLSGAARQAGIPLLTKPVRPARLRAALDSLL
                     KAQA"
     gene            complement(5406..>5993)
                     /locus_tag="VIS73_06270"
     CDS             complement(5406..>5993)
                     /locus_tag="VIS73_06270"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEY5762793.1"
                     /translation="VFDRVLRGASVRASALFLGQSFARQLAVVRKAFPDTRRVGVLAG
                     PESAQAIPLLEKAAREAGLSLLVERSDGGRLHGALRSIIASAHVLLVLPDPQVFNASS
                     IQGILLETYRAKIPLIGISSAYTRAGAALSLSVSPVQLGSETARLVRQVIEGDLPSPR
                     YSEAAEVHVNRQVVRSLGLNLPTDESLLSAARGVP"
BASE COUNT         1146 a         2052 c         1869 g          926 t
ORIGIN      
        1 catgatcctc gacgacggag gcgacgcaac gctgctgctg catctgggcg cccgcgccga
       61 aaaggacccc tcggttctcg gcaagccagc ctcggaagaa gagcgcgtgc tcttcgcagc
      121 gatcaagaag cgcaacgccg aacagcctgg ctggtacgcg aagaacctcg aggcaatccg
      181 cggcgtcacc gaagaaacga ccaccggcgt gcatcgcctg taccagatgc accagcgcgg
      241 cgaactcaag ttcccggcga tcaacgtcaa cgattcggtg accaagtcga agttcgacaa
      301 cctctacggc tgccgcgaat cgctggtcga cggcatcaag cgcgccaccg ccgtgatgat
      361 cgccggcaag gtcgccctgg tcgccggtta cggcgacgtc ggcaaggggt ctgcacaggc
      421 actgcgcgcg ttgtcggcgc aggtctgggt taccgagatc gaccccatct gcgcgctgca
      481 ggcggcgatg gaaggttacc gcgtggtgac gatggattac gccgccgaca aggccgacat
      541 tttcgtcacc gccaccggca actaccacgt catcggccac gagcacatgg cgaagatgaa
      601 ggatcaggcg atcgtctgca acatcggtca tttcgacaac gagatcgacg tcgcctcgat
      661 cgagggctat ccgtgggaag agatcaagcc gcaggtcgac cacatcacca tgccctcggg
      721 caacaagatc atcctcctcg ccaagggtcg cctggtgaac ctcggctgcg cgacgggtca
      781 tccgtcctac gtgatgtcgt cgtctttcgc caaccagacg atcgcgcaga tcgagctgtg
      841 gaccaagcgc gactcgggcg attacccggt cggcgtctac acgctgccca agcatctcga
      901 cgagaaggtc gcccgcctac agttgaagaa gctcaattcg cagctgacca cgctgacgaa
      961 agagcaggcc gactacatcg gcgtgccggt cgaaggtccg tacaagtccg accactaccg
     1021 ctactgatcg agctcggccg cgcgcgcctg ccccgcgcgc ggccgttgcg gctgcagcat
     1081 ctgccgattg tcgtgggcta cacttgtcat gtcatttgcg cacaggcagc acgaccacga
     1141 tgagccggaa aaagcccacg attcgccata cgcgtgaaag cgatgtcgac ccgctgatcg
     1201 ccctgcagaa ggaaacctac ccgacgatcc cgccctggcg ccgcgagcgg ctgctgaatc
     1261 agctggaagt ttttccgcag ggacaaatcg tcgccgaact caatggagaa atcgtcggcg
     1321 ccgcgagttc gctggtagtc ctctgggacg agtggctgac cgatcacaac tggagcgaaa
     1381 tcaccgcaag cggcagcttc aacacccata acccggacgg gcgaacgctc tacggcgccg
     1441 aggtcttcgt ccatccctac ctgcgcggcg ccggcatcgg tcacgcgctg tacaaggggc
     1501 ggcgcaagct ttgccgcgcg atgaatctca aacgcatcat cgcctgcggt cgcctgcccg
     1561 gttaccattt gcacgccgag aatatgtcgg ccgagcttta cgcgcaaaag gtggtctggg
     1621 gcgatctgcg cgacccggtg ctgagcttcc agctcaagga aggcttcagc tactgcggca
     1681 ccgtcgacga ctacataccc gaggacaagg aaagcctcgg ccacgcctcg ctgatcgtct
     1741 ggctgaacat gcgctacaac gccaagaagc cgaccaagat tccccaagga gacctgctat
     1801 gaaggttcgc atagccgcgg ttcagtacct gttacgtgcc atcgccgact gggacggctt
     1861 cgagaaccag gtccgtttcg tgatgaaggc ctgcggtgac taccggccgc acttcgtgat
     1921 gctgcccgag atcttcacca gccagctgct ctccttcctc gacgacggcg acatcaaggg
     1981 cgccgtgcgc cgcatgaacg acttcacacc acgctacatc gacctgatga ccgagctgtc
     2041 gaagaagtac ggtttctacc tgatcgccgg cagccatccc aacctgcgcg acgatcagct
     2101 ctacaacacg gccttcatgt tctcgccgcg cggcgacgtc tatacgcagg acaagattca
     2161 ccgtacgcgc tgggagcgcg agaaatggga caccgcggct ggcgacaagc tgtcgctgtt
     2221 tcagaccgaa tacggtgcga tcgcgattct catctgctac gacatcgagt tccccgaact
     2281 cgcgcgcatg gtctgcgaag cgggcgccga catcctcttc gtgccctcat cgaccgacga
     2341 ccgccagggt ttctggcgcg tgcgctactg tgcgcaggcg cgcgcaatcg agaatcaggt
     2401 ctacgtcgcc atgaccagca cggtcggcaa cctgccggtc gaaggcctcg gcctgcacta
     2461 cggtcaggcg agcatcctga cgccctccga cttccccttc gcgcgcgatg gcatcgccgc
     2521 cgagggcgtg gtcaacatgg aacagatcat tatcgccgac gtcgacctcg acgcgctgat
     2581 gctcaaccgc agcaacggga cgacgatccc tctttacgac aagcgccgcg aggtctacga
     2641 caaccccgtc gagaccatca gcacccgtta accacccgcc gaacgccacg atgccaaatc
     2701 gccttgtacc gatctcctgc gagttcttcc cgccacagac cgacgaaggt atcgccaagc
     2761 tgcgcgccac gcgcgaggca ctgacggcgc tggcccccga gttcttctcg gtcacctacg
     2821 gcgccggcgg ctcgactcgc gagcggacct tcgaggcggt cttcgacatc cacgccgccg
     2881 ggcagacggt ggcgccgcac ctctcgggca tcggctcgag tcgcgcgacg atccgcgaga
     2941 ttctcgccgc ctatcgcgac cgcggcatcc gccgcctggt cgcgctgcgc ggcgatctac
     3001 cctcggggat ggccgcctcg ggcgagttcc gctacgccag cgagctggtc gagttcatcc
     3061 gtgccgaaac cggcgactgg ttcaccatcg aagtcgccgc ctaccccgag tggcacccgc
     3121 aggcacgcac gccggccgac gacctcaagg ccttcgctgc caaggtcgct gctggcgccg
     3181 attcggcgat cacccagtat ttctacaatg ccgacgcgta ctttcgcttc gtcgatgacg
     3241 cacgaacggc gggcatccgg gtaccgatcg tccccggcat catgccgatc accagcttca
     3301 gcaaactggc gcgcttctcg gatgcctgcg gcgccgagat cccgcgctgg ctgcgctgga
     3361 agctcgaagg tttcggcgat gacagcgcgt cgattcgcgc gctgggactc gatgtggtca
     3421 ccgcgctctg cgaacggctg ctcgccggcg gcgcgcccgg aattcacttc tacacgatga
     3481 accaggccgc cgcgacgacc gcaatctgta cgcggctgcg gcttacgcct gcgccttgag
     3541 cagcgagtcg agcgcggctc gcagccgcgc cgggcgcacc ggcttggtca gcagcggaat
     3601 gccggcctgc cgcgctgcgc cggacagtcc gctgtccgcg ctgacgatga ccagcggcac
     3661 cgaatagccg agtcgcgtcg caatctcggc agcagtctgc ggaccgaatt cgccgccggt
     3721 gtggtcacag atcaaggcat ccagctttgc gtcggcgccg gccagattcg cgcccaattc
     3781 ttcgaggctc gcggcaccga cgctggaaca gccccacgac gccagcaggt cacgcatgcc
     3841 ggcaacgatg gcgacatcgt cctcgacaat tcccacccgg cggccggcca gcgacgatag
     3901 atcggcttct tgcgcatcgg tcggcagcgc gcgggcgggc ggcaactcaa accagaaaca
     3961 cgagccgcga ccgagcgccg aagacaagcc cagtcgatgg ccgagcagcg cgcaaagccg
     4021 ggcgacgatc gcgaggccga gaccgaggcc cttgcgctgg tctcgttcgg gattggcgag
     4081 ctggacgaac tcggaaaaca ccaacgcctg caaggcctcg ggaatgcccg tgccgctgtc
     4141 gcgcacttcg atgcggacca tcccgctgcg caggcgggcg gcgaccagga tgctgcctcg
     4201 ctcggtgtag cggatcgcat tggagatcag gttcatcagc acccgctcgc acatcaccgg
     4261 gtcgctctcc accgacagcg cgctcggcct gacccgcagg cgcagtcccg cggcgcgagc
     4321 cgactcgccg tattcggcct gcaagcggga aagcagggga gccagcgcga aaacgcgccg
     4381 attaggcacg aaggcaccgg cgtccagacg cgagatgtcg agcagcgaat cgagcagacc
     4441 tgccagcgac tcgctggctt cctgaatgcg gtgagccagc acgatggcct cgcccttaag
     4501 cggctgccgg ttgagctggt cggcgaagag ggtcagcgcg tgcatcggct ggcgcaggtc
     4561 gtggctcgcc gcagcaagga agcgcgactt cgcctcgttg gcgagttcgg cctcgcgctt
     4621 cttctccgac aactcggccg tcgcctcttc gacacggcgt tcgagttcgg tacgcgacgc
     4681 gccgagtcgc tcggccatgc ggttcactcc gttggccaag cggcgcatgt cgccgccgcc
     4741 ctcaggcggc agccgggcgc cgaaatcacc accgccgatc cgctcgatcg ccatgccgag
     4801 gcgtcgcaac gggccgacca gacgctcgcc gaagcgcagc gagagcaccg tcaccacggc
     4861 aaccacggtg gccgcaatcg tcgcgctcca gagcatgaag ccgcgcgcat cgcggcgcaa
     4921 cggttcgagc gagaggacca cctcgaccgc gccgatcggt ggcgactcgt cgaccggcga
     4981 cgatgcgaac agctcgtcga agacatcgct ggcgctgcgg cggaccagcc tgccgatcat
     5041 cacgctgtcg gtttcccagc gctcgccctc ggcgaaaccg gtcagtggca ccgcgcgccg
     5101 gacccgggtc tggtcgccga gcgaaaccag cgcgtcgcca cggccgtcgc ggatcgacac
     5161 cagcacgacg gcgcgttcgg tcaacgcgct gctggcgatc gattcgagga gatctcggtt
     5221 tcccgagaag aggccgaact cggcgttgga cgctagctgg cgggcaagca ggccgccaac
     5281 gtagaccatt tgcgcctggt cggccttcag ccgttcgcgt acgagaatgc cgccgatcgc
     5341 aatcgaggta aagagaactg gcagcaccgc aagcagcgta atgcgaccgc ggatgcccag
     5401 cggcctcacg gaacgccccg cgccgccgac aataggcttt cgtcggtcgg aagattgagt
     5461 ccgagcgagc gaacgacctg acgattgaca tgcacctcgg cggcctccga atagcgcggc
     5521 gacggcagat cgccctcgat cacttggcga acgagccgcg cggtttcgga cccgagctgc
     5581 actggggaca cggaaagcga tagcgccgca ccggcgcgcg tgtaggcaga cgagatgcca
     5641 atcaggggaa tcttggcgcg gtaggtctcg agcaggattc cctggatcga actcgcattg
     5701 aatacctgcg ggtcgggcag gacaaggagt acgtgggcgc tggcgatgat agagcgcagc
     5761 gcgccatgca gacgcccgcc gtcgcttcgc tcgacgagca gcgatagacc cgcttcgcgc
     5821 gccgcctttt ccagtaaggg aatggcctgc gccgactcgg gtcccgccag cacaccgacc
     5881 cgacgggtgt cgggaaaggc cttgcgcacg acagccagct ggcgggcgaa cgattggccg
     5941 agaaacagag cgctggcgcg caccgacgcc ccccgtagga cccggtcaaa aac
//
LOCUS       DATDAA010000192         3254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_503656,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000192 DATDAA010000000
VERSION     DATDAA010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503656"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /gene="serB"
                     /locus_tag="VIS73_06275"
     CDS             <1..899
                     /gene="serB"
                     /locus_tag="VIS73_06275"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEY5762794.1"
                     /translation="GHSPAARRAARTPSRLSAALVDLVLVGRPIPTHLIDELAQLSQA
                     GAVTAMPPAAVRLRKAKPHPEIAARCAAARIDFAFVEPGRRLADFGLLATDMDSTLIS
                     IECIDEVADFAGRKAEVAAITETAMQGRIDYAESLRQRVALLAGLPERVLDQVFEERL
                     SLNPGAETLLHAARTAGLKTQLVSGGFSYFTERLKADLGFDYAAANQLEIVDGKLTGR
                     VLGKIIDAAGKARCVAETRDRLGLGRSQVITIGDGANDLLMMAESALSVAYHAKPVVR
                     EKASAKLDHAGLDGILALFEDA"
     gene            896..2068
                     /locus_tag="VIS73_06280"
     CDS             896..2068
                     /locus_tag="VIS73_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5762795.1"
                     /translation="MKRGTTLWLGVAAVGLAVGAAYWLRGDDRSGADAYRTEPVSRGD
                     IAQRISANGTLNPVQLVSVGTQVSGTVKAIRVDFNERVRKGQILLELDDALPAAQARQ
                     STANVLSAQAALDLATANEARQRDLVERRFVSPGELDQAVQQLRSAEAALAVARAQAE
                     RDRVNLAYTVIRSPIDGVVIDRVVAVGQTVAASFQTPELIRIAGDLRQMQIDTSFAEA
                     DIGQVKVGQAARFTVDAHADRNFDGRVTQIRLNPKSEQNVVTYNVVIAVDNRDGLLLP
                     GMTATVGLTVAERRDVLRLPNAALRYRPPAEATGDRQRPASPGRLANANAAADPSATV
                     WRLDAGKPVATPVSVGITDGRMTELLSDSPADGTPLIVGTVRGEAPRGRGSRFRLF"
     gene            2068..2769
                     /locus_tag="VIS73_06285"
     CDS             2068..2769
                     /locus_tag="VIS73_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5762796.1"
                     /translation="MADAVIATTGLGKTYPTADGGFVALSEVDLEISAGEFVAVTGAS
                     GSGKSTLMNLLGCLDRPSTGRYRLLGEDVATLGDDRLAALRNRQIGFVFQGFHLLPRL
                     TLSENVALPLVYAGVGAAERCRRAHEQLSRVGLLDWADAFPNRISGGQQQRTAIARAL
                     VNHPRLLLADEPTGNLDSHTSDEIMDLLGELNGDGVTLLVVTHELDIAARAGRQIVFR
                     DGRILSDVRQSSEQP"
     gene            2766..>3254
                     /locus_tag="VIS73_06290"
     CDS             2766..>3254
                     /locus_tag="VIS73_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5762797.1"
                     /translation="MKLLLLSEAWRAMRANRLRTALTMLGMVIGVAAVVAMLALGQGA
                     QQAVEGAARTMGANLFIVLSGSTSSGGARLGGGSAPTLSVDDATAIAELPDVGAAAPV
                     QTGSAQVIHGASNWNTAITATTPDYLIVRDWNLARGVPFANSDLRSATRVALIGQTVA
                     GNL"
BASE COUNT          538 a         1194 c         1038 g          484 t
ORIGIN      
        1 gaggccacag tccagctgcg cgacgtgctg cccggacgcc gtcgcgcttg agcgctgcct
       61 tggtggacct cgtccttgtc gggcggccga ttccgaccca cctgattgat gaactcgcgc
      121 agctgagcca ggccggcgcg gtgacggcaa tgccgcccgc agctgtgcgt ctgcgcaagg
      181 caaagccaca cccggaaatc gccgcccgtt gcgccgcggc acgcatcgac ttcgcgttcg
      241 tcgagcccgg ccgacgcctc gccgatttcg gcctgctcgc caccgacatg gactcgacgc
      301 tgatcagcat cgagtgcatc gacgaggtcg ccgattttgc cggtcgcaag gccgaggtcg
      361 cggcgatcac cgaaactgcg atgcagggcc gcatcgacta cgccgaaagc ctgcgccagc
      421 gcgtcgcgct gctcgccggc ctgcccgagc gcgtgctcga ccaggtcttc gaggaacggc
      481 tgagcctcaa tccaggcgcc gagacgttgc tgcacgccgc acgcactgcc gggctgaaga
      541 cccagctcgt ctccggtggc ttcagctact ttaccgagcg cctcaaggcc gacctcggct
      601 tcgactacgc cgccgccaac cagctcgaga tcgtcgacgg caagctcacc ggccgcgtcc
      661 tcggaaagat tatcgacgcc gccggcaagg cacgctgcgt cgctgaaacg cgcgaccggc
      721 tcggccttgg acgtagccag gtgataacca tcggcgacgg ggccaatgac ctgctgatga
      781 tggccgaatc ggcgttgtcg gtcgcctatc acgccaagcc cgtggtccgc gagaaagcca
      841 gtgcaaagct cgaccacgcg gggctcgacg ggattctcgc cctcttcgaa gacgcatgaa
      901 gcgcggcacg accctctggc tcggcgtcgc ggcagtgggg cttgctgtcg gcgcggctta
      961 ttggctgcgt ggcgacgacc gttcgggcgc ggatgcctac cgcaccgagc cagtcagccg
     1021 cggcgacatc gcccagcgca tctcggccaa cggcacgctc aatcctgtgc agctggtcag
     1081 cgtcggcacg caggtctcag gaaccgtcaa ggccatccgc gtcgatttca acgaacgggt
     1141 gcgcaagggc cagatcctgc tcgaacttga cgatgcgcta cccgccgcgc aagcccggca
     1201 atcgacggcc aacgtgctca gcgcgcaagc ggcgcttgac ctcgcaaccg ccaacgaagc
     1261 acggcagcgc gacctcgtcg aacgccggtt cgtttcaccg ggcgaactcg atcaggcggt
     1321 acaacagctc cggtcggccg aggccgcgct cgccgtcgcc cgggcccagg ccgaacgcga
     1381 ccgtgtcaat ctggcttaca cagtgatccg ctcgccaatc gacggagtcg tcatcgatcg
     1441 cgtcgtcgcc gtcgggcaga ccgtcgccgc cagcttccag accccggaac tgatccgcat
     1501 cgccggcgac ctgcggcaga tgcagatcga caccagcttc gccgaggccg acatcggtca
     1561 ggtcaaggtc gggcaggccg cccgcttcac agtcgatgcc cacgccgacc gcaacttcga
     1621 cggacgggtc acgcagatcc gcctcaatcc gaagagcgaa cagaacgtcg tcacctacaa
     1681 cgtcgtcatc gccgtcgaca accgcgatgg cctgctgctg cccggcatga ccgccactgt
     1741 aggcctaacg gtagccgaac gtcgcgatgt gctgcgcctg ccgaacgccg cgctgcgcta
     1801 ccgcccgccg gccgaagcca ccggcgatcg ccaacgcccg gctagcccgg gccgcctcgc
     1861 caacgccaac gcagcggccg acccgagcgc gacagtctgg cgcctcgacg ccggcaagcc
     1921 tgtcgccaca cccgtcagcg tcggcatcac cgacggccgc atgaccgaac tgctcagcga
     1981 ctcgcctgcc gatgggacgc cattgatcgt cggtaccgtg cgcggtgaag caccgcgcgg
     2041 acggggctcg cgcttccggc ttttctgatg gccgacgcgg tcatcgccac caccggcctc
     2101 ggcaagacct acccgacggc cgacggcggc ttcgtcgcgc tgagcgaggt cgacctcgaa
     2161 atttccgccg gcgagttcgt cgccgtcacc ggcgcctcgg gctcgggcaa gagtacgctg
     2221 atgaacctgc tcggctgcct ggatcggccg agcaccgggc gctatcgcct gctcggtgaa
     2281 gacgtggcga cgctgggcga cgaccgcctc gcagcactgc gcaaccgcca gatcggcttc
     2341 gtcttccagg gctttcacct gctgccgcgc ctgacgctca gcgagaacgt cgcgctgccg
     2401 ctggtctacg ccggcgtcgg cgccgccgag cgctgccggc gcgcccacga acagctgtcg
     2461 cgcgtcggcc tgctggattg ggcggatgcc tttcccaatc gaatctccgg cggccagcag
     2521 cagcgcacgg cgatcgcccg agcactggtc aatcatcctc gcctgctgct tgccgacgaa
     2581 ccgaccggca atctcgacag ccacaccagc gacgagatca tggacctgct cggcgagctg
     2641 aatggcgacg gcgtcaccct gctcgtcgtc acccacgaac tagacatcgc tgcgcgagcc
     2701 ggacggcaga tcgtctttcg cgacggccgc atcctcagcg acgtgcgaca gtcgtcggag
     2761 caaccatgaa gctgttgctg ctcagcgaag cctggcgggc gatgcgtgcc aaccgcctgc
     2821 gcacggcgct gaccatgctc ggcatggtca tcggcgtcgc tgccgtcgtc gccatgctcg
     2881 cgctcggtca gggcgcgcaa caggcggtcg agggcgcggc gcggacgatg ggcgccaatc
     2941 tgttcatcgt cctctcgggt tccacctcgt cgggcggcgc ccgcctcggc ggcggcagtg
     3001 cgccgacgct cagcgtcgac gatgcgacgg cgattgccga actgcccgac gtcggcgccg
     3061 cggcgccggt gcagactggc agcgcgcagg tgatccacgg cgccagcaac tggaacacgg
     3121 cgatcaccgc caccaccccc gactacctga tcgtccgtga ctggaatctg gcgcgcggcg
     3181 tgcccttcgc caactcggat ttacgctcgg cgacacgggt cgcgctgatc ggccagacag
     3241 tcgccggcaa cctg
//
LOCUS       DATDAA010000193         4026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_49060,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000193 DATDAA010000000
VERSION     DATDAA010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49060"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /gene="proB"
                     /locus_tag="VIS73_06295"
     CDS             complement(<1..668)
                     /gene="proB"
                     /locus_tag="VIS73_06295"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY5762798.1"
                     /translation="MSEQNSLPKARRLVVKVGSALVTNNGAGLDLAAIAEWGRQIAAL
                     RADGREVLLVSSGAIACGMQRLGWTKRPHAVHKLQAAAAVGQMGLAQAYEGIFREHGL
                     GTAQVLLTHDDLADRKRYLNARSTLLTLIEFGVVPVINENDTVITDEIKFGDNDTLGA
                     LVANLVEADALIILTDQSGLYTADPRQDPQATLVRHGLAGDPALERMAGGAASQLSRG
                     GMLT"
     gene            complement(661..1776)
                     /gene="obgE"
                     /locus_tag="VIS73_06300"
     CDS             complement(661..1776)
                     /gene="obgE"
                     /locus_tag="VIS73_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY5762799.1"
                     /translation="MKFIDEASILVIAGKGGDGSAAMRREKYVPRGGPSGGDGGRGGS
                     VYAEADCNLNTLVEFRFTKRFAARQGENGGAKDCYGRGAEDIVLRVPVGTAVTDSETG
                     EPVADLDQHGKRALLAKGGQGGLGNIHFKSSTNRAPRQFTRGTPGEERALRLELKVLA
                     DVGLLGLPNAGKSTFIRAVSAARPKVADYPFTTLHPNLGVVRVDQDRSFVVADIPGLI
                     EGAADGAGLGIRFLKHLQRTRLLLHIVDLVPPDPEADPVRDALAIVGELAAYSADLAN
                     KPRWLVLNKIDLLPAGERDARVAAFVDAYRQAGGLVDPHFVIAAIKGDGCSAVTYAIM
                     DYLDTVPRPVLDAADDEEPADALVDGEAGVAPEDGDE"
     gene            complement(1864..2121)
                     /gene="rpmA"
                     /locus_tag="VIS73_06305"
     CDS             complement(1864..2121)
                     /gene="rpmA"
                     /locus_tag="VIS73_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY5762800.1"
                     /translation="MAHKKAGGSSRNGRDSESKRLGVKRYGGQEVLAGNILVRQRGTQ
                     FHPGDNVGIGKDHTLFALVDGRVAFTVRGPQRRRTVSISNA"
     gene            complement(2149..2460)
                     /gene="rplU"
                     /locus_tag="VIS73_06310"
     CDS             complement(2149..2460)
                     /gene="rplU"
                     /locus_tag="VIS73_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY5762801.1"
                     /translation="MYAVVKTGGKQYRVTTGEKLKVEQIPADVGAEVTLDQVLMVGEG
                     ESVKIGAPLVSGASVKATVVSHGRHDKVRIFKMRRRKHYQKHQGHRQNFTELRIDAIN
                     A"
     gene            2639..3238
                     /locus_tag="VIS73_06315"
     CDS             2639..3238
                     /locus_tag="VIS73_06315"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY5762802.1"
                     /translation="MAVLVGKPAPDFTAEAVLGNNEIKTLKFSEHIRGKYAVLFFWPL
                     DFTFVCPSELIAFDHRLAEFKKLGVEVVGCSIDSHFTHLAWKNTPVNSGGIGQVGYPM
                     VADIKHDICRAYDVEAADGVAFRGSFLIDKAGIVRHQVVNDLPLGRNIDEMLRMVDAL
                     QFTEEHGEVCPAGWAKGKAGMKASADGVASYLTEHAKEL"
     gene            complement(3302..>4026)
                     /locus_tag="VIS73_06320"
     CDS             complement(3302..>4026)
                     /locus_tag="VIS73_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762803.1"
                     /translation="GQHEQRISTRSEALLRGVRIAELWRDPESGQQHALAVLPRLQAA
                     QGLRRELVQLDDGLSRQLAAAQAAGDPLAQLGPAERALRLAEERETLDRMLRVVDSTG
                     MGMPPANGLARLRADRDALVARVRVVVDSTEGDADFAETIRGALAKSGFTVARDDAAA
                     EYRLAATLDAHDLGQQEGWNWLRASVSIVLKDASDRVRGQHSWSFKASAADPRSARQR
                     LHKDIERVLGEELLPVIVGFAG"
BASE COUNT          704 a         1379 c         1242 g          701 t
ORIGIN      
        1 ttggtcagca tgccgccacg cgagagttgg ctggcagcgc cgcccgccat gcgttcgagc
       61 gccggatcgc cggccaggcc gtgacgaacc agcgtcgcct gcggatcctg gcgcggatcg
      121 gccgtgtaca acccggactg gtcggtgaga atgatcagcg cgtcagcctc gaccaaattg
      181 gccaccagcg cgcccagcgt gtcgttgtcg ccaaacttga tctcgtcggt aatcaccgtg
      241 tcattctcgt tgatcaccgg caccacgccg aactcgatca gcgtcaacag ggtcgagcgg
      301 gcattgagat agcgcttgcg gtcggccagg tcgtcgtggg tcagcagaac ctgcgctgtg
      361 cccagcccgt gctcgcggaa gatcccttcg taggcctgcg ccaagcccat ctggccgacg
      421 gcggcggcgg cctgcagctt gtgtactgcg tgcgggcgct tcgtccatcc cagccgctgc
      481 atgccgcagg cgatcgcccc cgaggacacc agcagcacct cgcgcccgtc ggcacgcagc
      541 gccgcgatct ggcggcccca ctcggcaatc gccgcaaggt cgagaccggc gccgttgttg
      601 gtgaccagcg cgctgccaac cttgaccacc agccgacggg ccttcggcag cgaattctgc
      661 tcactcatcg ccatcttccg gcgcgacgcc cgcctctcca tcgaccagcg catcggccgg
      721 ttcttcatcg tccgccgcat cgagcaccgg ccgcggcacg gtgtcgaggt aatccatgat
      781 cgcgtaggtg acagcggaac agccatcgcc cttgatcgcg gcgatgacga aatgcggatc
      841 gacgaggccg ccggcctggc gataggcatc gacaaaggcc gcaacgcggg catcgcgctc
      901 cccggcgggc agcaggtcga tcttgttcag cacaagccaa cgcggtttat tcgcaagatc
      961 agcggaataa gccgccagtt cgccgacgat cgccagcgcg tcgcgaaccg gatcggcctc
     1021 gggatcgggc ggcacgagat cgacgatgtg cagcagcaga cgcgtgcgct gcaggtgctt
     1081 gagaaagcgg atgcccagtc cggcgccatc ggccgcgcct tcgatcagcc cggggatgtc
     1141 ggcgacgaca aagctgcgat cctgatcgac gcgaacgacg ccgaggttgg gatgcagcgt
     1201 cgtgaacgga tagtcggcga ccttcgggcg ggccgccgaa acggcgcgga tgaaagtgct
     1261 cttgcccgca ttgggcaggc cgagcagacc aacgtcggcg agcaccttga gttcgaggcg
     1321 cagggcgcgc tcttcgcccg gcgtgccgcg ggtgaactgt cgcggcgcgc ggttggtgct
     1381 cgacttaaag tggatgttgc cgagtccgcc ctgcccgccc ttggccagca gcgcgcgctt
     1441 gccgtgctga tcgagatcgg cgaccggctc gccggtctcg gaatcggtga ccgcggtgcc
     1501 gaccggcacg cgcagcacga tgtcctcggc gccgcgacca tagcagtcct tggcgccgcc
     1561 gttctcacct tggcgggcgg cgaaacgctt ggtgaagcgg aattcgacga gcgtattcag
     1621 gttgcagtcg gcctcggcgt agacgctgcc gccacggccg ccgtcgccgc ccgacggccc
     1681 gccgcgcggg acatacttct cgcgacgcat cgccgccgaa ccgtcgccgc ccttgccggc
     1741 gatgaccagg atcgatgcct catctatgaa tttcacgtta cgctccgcgc ctgcctgcca
     1801 atgccattcc gcaacaaaaa agccctatcg gacgataggg ctttttgccg caccacgtcc
     1861 tgctcaagcg ttgctgatgc tcaccgtgcg ccgacgctgc gggccgcgaa ccgtgaaggc
     1921 aacccgcccg tcgaccagcg cgaacagcgt gtggtccttg ccgatgccga cgttgtcgcc
     1981 cggatggaac tgcgtaccac gctggcgaac gaggatgttg ccggcgagga cttcctgacc
     2041 gccgtagcgc ttgacgccca ggcgcttcga ctccgaatcg cgaccgttac gtgaactgcc
     2101 gcctgctttc ttgtgtgcca tggttcagac tcctaatgac tgtcgcgctc aggcgttgat
     2161 cgcgtcgatg cgcagttcgg tgaaattctg ccggtgcccc tgatgcttct gatagtgctt
     2221 ccgacggcgc atcttgaaga tgcggacctt gtcgtgacga ccgtgcgaga cgaccgtcgc
     2281 cttgaccgaa gcacctgaaa cgagcggggc gccgattttc acgctctcgc cttcgcctac
     2341 catgaggacc tggtcgaggg tgacttctgc gccaacgtcc gccggtatct gttctacttt
     2401 aagtttttcg ccagtggtca cgcgatactg cttgccaccg gtttttacga ccgcgtacat
     2461 gttggactcc gaatgaaagc gccccaaagc gggacgaccc tgaaaaagaa ccgcgcattc
     2521 taggcaaaaa ggccagaaca atcaacgtcc ccatgcgcga caccgaaccg gcgacgattg
     2581 acccactccc cttgcctacc taagatcgcc tccgccaacc aacaaactgg agaaaaaaat
     2641 ggctgttctc gtcggcaaac ccgcccccga cttcactgcc gaggccgtac tcggcaacaa
     2701 tgaaatcaag accttgaagt tttccgagca cattcgcggc aagtacgcgg tgctcttctt
     2761 ctggccgctt gatttcacct tcgtttgccc ctcggagctg atcgccttcg atcaccggct
     2821 tgccgaattc aagaagctcg gcgtcgaagt cgtcggttgc tcgatcgact cgcacttcac
     2881 gcacctcgcc tggaaaaaca ccccagtcaa ttccggcggt atcggccagg ttggctaccc
     2941 gatggttgcc gacatcaagc acgacatctg ccgtgcctac gacgtcgaag cagctgacgg
     3001 cgtcgccttc cgtggctcct tcctgatcga caaggccgga atcgtccgcc accaggtggt
     3061 caacgacctg ccgcttggcc gcaacatcga cgagatgctg cgtatggtcg acgcgctgca
     3121 gttcaccgag gaacacggcg aggtctgccc ggccggttgg gccaagggca aggccggcat
     3181 gaaggcttcc gccgacggcg tcgccagcta cctgaccgag cacgccaagg aactctgagc
     3241 aagtccccga gacatcggca aagcccgacc gcccgcggcc ctagcccggg cggttttcgt
     3301 ttcagccggc gaaaccgact attaccggca gcaactcctc tccaagaacg cgctcgatgt
     3361 ccttatgcag gcgttgccgt gcactgcgcg gatcggcggc ggacgccttg aaactccagg
     3421 aatgctggcc gcggactcgg tcactggcgt ctttaaggac gatgctgacg cttgcccgca
     3481 gccagttcca gccttcctgc tggccaaggt cgtgcgcgtc gagggtcgcc gccaggcggt
     3541 attcggcggc cgcgtcatcc ctggcgacgg tgaaacccga cttcgccaaa gcgccacgga
     3601 tggtctcggc aaagtctgcg tcgccctcgg tggaatcgac gaccacgcgg actcgcgcaa
     3661 ccagcgcatc gcgatcggcg cgcaggcgcg ccagaccgtt tgccggcggc attcccattc
     3721 ctgtcgaatc gaccacgcgc agcatgcggt cgagcgtctc gcgttcctcg gcaagccgca
     3781 gcgcccgctc ggccgggccc agctgggcta gcggatcacc ggccgcctgc gcggcagcga
     3841 gttgccggga tagcccgtcg tcgagttgga cgagttcgcg tcgcaatccc tgcgccgcct
     3901 gcaaccgcgg caacaccgcc agcgcatgct gctgcccgga ttccgggtcg cgccacagtt
     3961 cggcaatgcg cacgccgcgc agcagcgcct cgctgcgcgt cgagatgcgc tgctcgtgct
     4021 gcccgg
//
LOCUS       DATDAA010000194         5647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_84153,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000194 DATDAA010000000
VERSION     DATDAA010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84153"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2398
                     /gene="mutS"
                     /locus_tag="VIS73_06325"
     CDS             <1..2398
                     /gene="mutS"
                     /locus_tag="VIS73_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY5762804.1"
                     /translation="AGVPQHSVEQYLARLVRLGESVVICEQIGDPATSKGPVDRAVSR
                     IVTPGTITDAALLEDKRDSHLAAISRGRKQAGIAWLNLVTGELRLTELPLDRLGAWFE
                     RIRPAELLLPDSTASREGGDDWIAVDDLPRRLLPASQFVSDSGARRLGEQYGVAAIDG
                     LGLPIDGPAIGAAGALLAYATATQAQSLQHLRPPIVERDDEFLMLDAATRRNLELTET
                     IRGEPAPTLFALLDRCATAMGSRLLRHALHHPLRSRALPEARSAAIAAIISNSAAARI
                     GEALAGIADIERIAGRIALRSVRPRELSGLREGLRRLPTLAERLGSIDSTLIDSIRAT
                     VLTPQATLSLIADAILEEPAALIRDGGVIASGHDADLDDLRAISEDCSAFLLDLEARE
                     RQRTGIANLRVEYNRVHGFYIEVGRAQSGKVPEDYRRRQTLKNAERYVTPELKAFEDK
                     ALSARDRALAREKWLYEALVERLCEQVPSLQRLAEAVATLDMLAALARVAEDYGWCRP
                     ELVDERCVDIEAGRHPVVERQLATQGIRFVANDARLLADRHLLLVTGPNMGGKSTYMR
                     QIAHIALLAMIGSFVPAARARIGPLDAIFTRIGASDDLASGRSTFMVEMTESAAILNQ
                     ATENSLVLMDEIGRGTSTFDGMALAFAICRHLVQVNRSLALFATHYFELTQLAAENPA
                     LRNVHLDAVEHGERIVFLHAVEDGPANQSYGIQVAALAGIPKPVIRAARRTLQALEQR
                     SVNDTRQPDLFAASTEMDVPEAVPDALREAVDAIDPDDLSPREALDLIFRLKKLRAAD
                     "
     gene            complement(2418..2915)
                     /locus_tag="VIS73_06330"
     CDS             complement(2418..2915)
                     /locus_tag="VIS73_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5762805.1"
                     /translation="MQVVKDTVVTLDYSVADPDGHVVDPGREPLVYLHGGHDDIFPKI
                     EEALHGKRVGDTVHVKLQPEEAFGEYDPELVQVEERSKFPETLEVGMQFEGSEELTDD
                     DDDLVIYRVTEIAEDRVVLDGNHPLSGTALVFTCTVTAVRPASQDEMSHGHVHGDEDD
                     EPTRH"
     gene            complement(2930..3397)
                     /locus_tag="VIS73_06335"
     CDS             complement(2930..3397)
                     /locus_tag="VIS73_06335"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY5762806.1"
                     /translation="MPQIGRKAPDFSLPDAEMEIVSLSALLGKNVVLFFYPKDDTPGC
                     TTEATEFSDHDSDFTACNCVVIGINRDDCMKHAAFRDKHGLSVPLLSDVEGEVCQLYG
                     VLQEREVDGVKRIGVARTTFVIDKQGVIRQVFQSVTPRGHALEVLACVRGLGK"
     gene            complement(3501..3851)
                     /gene="ftsB"
                     /locus_tag="VIS73_06340"
     CDS             complement(3501..3851)
                     /gene="ftsB"
                     /locus_tag="VIS73_06340"
                     /inference="COORDINATES: protein motif:HMM:NF002058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEY5762807.1"
                     /translation="MRWLSILLITLLVLVQYPLWFGKGGWLRVRDSERRLAEQETINR
                     KLEARNQALDAEVRDLKQGYEAIEERARFELGFVRDGERFVQFRNPAMPPPAAPLPRQ
                     ETVVGNDGEAREKP"
     gene            complement(3926..5209)
                     /gene="eno"
                     /locus_tag="VIS73_06345"
     CDS             complement(3926..5209)
                     /gene="eno"
                     /locus_tag="VIS73_06345"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY5762808.1"
                     /translation="MSSIVDVVAREVLDSRGNPTVECDVLLESGSMGRAAVPSGASTG
                     SREAIEMRDGDTARYLGKGVLRAVENVNTEISEAIIGLDAQEQAFIDKTMIDLDGTDN
                     KSRLGANAILAVSMAVAKAAAEESGLPLYRYFGGSGPMQMPVPMMNIINGGAHANNSL
                     DIQEFMVMPVGQSSFREALRCGAEIFHALKKLLDKKGHSIAVGDEGGFAPNLASHAEA
                     VQFIIQAIDAAGYTAGEDVLIALDCASSEFFKEGRYHISGEGLQLSSEQFTDYLANLA
                     DQFPIVSIEDGMAEGDWDGWKMLTDKLGKRIQLVGDDLFVTNTKILKEGIQKGVANSI
                     LIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGMNALQIKTGSL
                     SRSDRIAKYNQLLRIEEDLGDTAGYPGRDAFYNLR"
     gene            complement(5232..5546)
                     /locus_tag="VIS73_06350"
     CDS             complement(5232..5546)
                     /locus_tag="VIS73_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY5762809.1"
                     /translation="MKKGYLIVRADVRDAGAYEVYKQLAEQAVRLYGGHYLVRGGALE
                     VLEGSSPPPGRLVIIEFESVAQARRFYESAEYRKARESRADIADMDMHVVEGVSLDGG
                     NG"
BASE COUNT         1033 a         1887 c         1704 g         1023 t
ORIGIN      
        1 ggccggcgtc ccgcagcatt cggtcgagca gtacctggcg cgcctggtcc ggctcggcga
       61 atcggtggtg atctgcgagc agatcggcga cccggcgacc agcaagggac cggtcgaccg
      121 cgctgtctca cgaatcgtca cgccggggac gatcaccgac gcggcgctgc tcgaggacaa
      181 gcgagacagc catctggcgg cgatcagccg tggccgaaaa caagccggta ttgcctggct
      241 caatctggtg accggcgaac tgcggctgac cgagttgccg ctcgaccgtc tcggcgcctg
      301 gttcgaacgg atccgccccg ccgaactgct gcttcccgac agcaccgcca gccgcgaagg
      361 cggcgacgac tggatcgccg tcgatgatct gccgcggcgt ctgctgccgg catcgcagtt
      421 cgtcagtgac agcggtgcac gccggctcgg cgagcagtat ggtgtcgcgg ccatcgatgg
      481 gcttggtctg cccatcgacg gtccggcgat cggcgccgcc ggcgccctgc ttgcctacgc
      541 cacggcgacc caggcgcagt cgctgcagca cctgcggcca cccattgttg agcgtgacga
      601 tgaattcctc atgctcgatg ccgcgacgcg gcgcaatctc gaactcaccg agacgattcg
      661 cggcgaaccg gcgccaacgc tcttcgccct gcttgatcgc tgtgccacgg cgatgggctc
      721 acgcctgctg cggcacgcgc tgcaccatcc gctgcgaagc cgcgccttgc ccgaggccag
      781 aagcgccgcg atcgcggcga tcatcagcaa tagcgcagcc gctcggatcg gcgaagcgct
      841 ggcggggatc gccgatatcg aacgaatcgc cgggcgaata gccttgcgca gcgtccgacc
      901 gcgcgagctc agtggcctgc gcgaaggcct gcgccggctc cccacccttg ccgaaaggct
      961 tgggtcgatc gactcgacgc tcatcgattc aatccgcgcg accgtgctca cgccgcaggc
     1021 aacactgagc ctgatcgccg acgcgattct cgaagagccg gccgccctga ttcgcgacgg
     1081 tggcgtcatc gccagcggcc acgacgctga tctcgacgat ctgcgcgcaa tcagcgaaga
     1141 ctgcagcgcc ttcctgctcg acctcgaggc acgcgagcga cagcggaccg gcatcgccaa
     1201 tctgcgcgtc gagtacaacc gcgtgcacgg cttctacatc gaggtcggcc gtgctcagtc
     1261 gggcaaggtt cccgaggatt accggcgtcg gcagacgctg aaaaacgccg agcgctacgt
     1321 cacgccggaa ctcaaggcct tcgaggacaa ggcgctgtcg gcgcgcgacc gggcgctggc
     1381 gcgcgagaag tggctttacg aagcgctggt cgagcgcctg tgcgagcagg tgccgtcgct
     1441 gcaacggttg gccgaggcgg tcgcaacact cgacatgctg gcggcgctgg cccgcgtcgc
     1501 cgaggactac ggctggtgcc gtcccgaact cgtcgacgag cgctgcgtcg acatcgaagc
     1561 cgggcgccac ccggtcgttg aacggcaact ggcgacgcag ggcatccgct tcgtcgccaa
     1621 cgatgcgcgc ctgctcgctg atcgccatct gctgctggtc accggcccga acatgggcgg
     1681 caaatcgacc tacatgaggc agatcgcgca catcgcgctg ctggcgatga tcggtagttt
     1741 cgtccctgcg gcgcgcgcac ggatcggccc gctcgacgcc atcttcacgc gcatcggcgc
     1801 ctccgacgac ctggcatcgg gtcgttcgac cttcatggtt gagatgactg aatcggcggc
     1861 catccttaat caggcgaccg agaacagcct tgtgctgatg gacgaaatcg gccgcggcac
     1921 ctcgaccttc gacggcatgg cgctggcgtt cgcgatctgt cgtcacctcg tgcaggtcaa
     1981 ccgcagccta gcgctgttcg cgacccacta cttcgagctg acccagctcg ccgccgaaaa
     2041 ccctgccctg cgcaacgtgc atctcgacgc cgtcgaacac ggcgagcgaa ttgtcttcct
     2101 gcatgcggtc gaggacggac cggccaacca gagctacggc attcaggtcg cggcgctcgc
     2161 gggaattcca aaaccggtca ttcgcgcggc gagacggacg ctgcaggccc tcgagcaacg
     2221 cagcgtcaac gacacgcgcc agccggacct gttcgccgcc agtaccgaaa tggatgtgcc
     2281 agaagcggta ccggacgcgc tacgcgaggc ggtcgatgca atcgatcccg acgacctgag
     2341 tccgcgcgag gcgctcgacc tgatcttccg cctgaagaaa ctgcgcgccg ccgactaaag
     2401 ccgcaagagc cgccgcttca gtggcgggtc ggttcgtcgt cctcgtcgcc gtgcacgtgg
     2461 ccgtggctca tttcgtcctg gctggccggg cgcaccgcag tcacggtgca ggtaaacacc
     2521 agcgccgtac ccgaaagcgg gtgattgccg tcgaggacca cgcgatcctc ggcgatttcg
     2581 gtgacgcgat agatcaccag gtcgtcatca tcgtcggtca gctcttcgct gccttcaaac
     2641 tgcatgccga cttcaagcgt ctccggaaac ttgctgcgtt cctcgacctg aaccagctcg
     2701 gggtcgtatt caccgaaggc ctcctccggt tgcagcttga cgtgcaccgt gtcgccgaca
     2761 cgcttgccgt gcaaagcctc ttcgatcttc gggaagatgt cgtcgtgccc accgtgcagg
     2821 tagacgagcg gctctcggcc cgggtcgaca acgtgtccat cgggatcggc aacgctgtaa
     2881 tccagtgtta cgaccgtgtc cttgactacc tgcattccat tcccctttgc tatttgccga
     2941 ggccgcgaac gcaggcgagt acctcgagcg cgtgcccccg tggcgtgacc gactggaaga
     3001 cctgtcggat gacgccctgc ttgtcgatga cgaatgtcgt ccgagcgacc ccgatccgtt
     3061 tgacgccgtc aacttctctc tcctgcaata cgccgtaaag ctggcagacc tcgccttcaa
     3121 catccgacag cagcggcacc gacaagccat gcttgtcgcg aaacgcggcg tgcttcatgc
     3181 agtcatcgcg attgatgccg atcaccacgc aattgcaggc ggtgaaatcg ctgtcgtgat
     3241 cactgaactc cgtggcttca gtcgtgcatc ccggcgtgtc gtccttggga tagaaaaaca
     3301 ggacgacgtt cttgccaagc aatgcggaca gcgaaacgat ttccatctcc gcatcgggca
     3361 acgaaaaatc cggcgccttg cgaccaatct gtggcatgcg cgctccttgt tgtgtcgttc
     3421 gactccattg ggcaactgga ttctagcgga agaagcgcac atcggttgcg cgcgcgtcgc
     3481 aagttgcaag agccggtcga ctatggtttc tcgcgcgcct ctccgtcgtt gccaaccacc
     3541 gtttcctgac gtggcagcgg tgccgcgggc gggggcattg ccggattgcg aaactgcacg
     3601 aagcgctcgc cgtcacggac gaatccgagt tcgaagcgcg cgcgctcctc gatcgcttcg
     3661 taaccctgct tgaggtcgcg cacctcggca tcgagtgcct ggttgcgcgc ttcgagcttg
     3721 cgattgatgg tctcctgctc ggccagccga cgctcgctgt cacgcacgcg cagccagcca
     3781 cccttaccga accagagcgg gtactgcacg agaaccagca gcgtgatcag cagaatgctc
     3841 agccagcgca aggcacttca ctccgatagc gaaaaggccg caaagcctgc aagagacttt
     3901 gcggcccggg cttgcgcacc agcaatcagc gcaggttgta gaacgcgtcg cgccccgggt
     3961 aaccagccgt atcgccgagg tcctcctcga tccgtaacag ttggttgtac ttggcgatgc
     4021 gatccgaacg tgacagggac ccggtcttga tctgcagcgc gttcatgccg acggcgatgt
     4081 cggcaatcgt gctgtcctcg gtctctcccg agcgatgcga gatcaccgcc gtataaccgg
     4141 cacgcttggc catctcgatc gcagcgaaag tctcagtgag cgtaccgatc tggttgattt
     4201 tgatcaggat cgaattggcg acgcccttct ggatgccttc cttgagaatc ttcgtattgg
     4261 tgacaaaaag gtcatcgcca accagctgga tccgcttgcc gagcttgtcg gtgagcatct
     4321 tccagccatc ccagtcgcct tcggccatgc cgtcctcgat cgagacgatc ggaaactggt
     4381 cggcgaggtt tgccagatag tcggtgaact gttccgacga caactgcaga ccctcaccgg
     4441 agatgtgata gcgaccttcc ttgaaaaact ccgaactggc gcagtcaagc gcgatcaaca
     4501 cgtcctcgcc ggcggtataa ccggcagcgt cgatcgcctg gatgatgaat tgcacggcct
     4561 cggcgtggct ggcgagattc ggcgcaaagc caccctcgtc gccgacggca atcgaatgac
     4621 ccttcttgtc gagcaatttt ttcagcgcat ggaagatttc ggcgccgcaa cgcagcgcct
     4681 cgcggaagga cgactgcccg accggcatga ccataaattc ttggatatcg aggctgttgt
     4741 tggcgtgggc accgccgttg atgatattca tcatcggcac cggcatctgc atcggccccg
     4801 aaccgccgaa ataacggtag agcggcagac ccgattcctc ggcagcggcc ttggccacgg
     4861 ccatcgacac ggcaagaatc gcgttggcgc cgagtcgcga cttgttgtcg gtgccgtcaa
     4921 ggtcgatcat cgtcttgtcg atgaaggcct gctcctgcgc gtccagtccg atgatcgcct
     4981 cggagatctc ggtgttgacg ttctccaccg cacgcaggac acccttgccg agatagcgtg
     5041 ccgtatcgcc gtcgcgcatt tcaatcgcct cacgcgagcc agtcgacgcg ccactcggca
     5101 cggctgcacg gcccatcgaa cccgactcca gcaggacatc gcattccacg gtgggattgc
     5161 cacgcgaatc aagaacctca cgcgcgacta catcgacaat cgaactcatt tccttcccct
     5221 ctgcaattga atcaaccgtt tccgccgtcc agcgatacac cttcgacgac gtgcatgtcc
     5281 atatcggcga tatccgcgcg gctctcgcgc gccttgcggt actcggccga ctcgtagaac
     5341 cggcgtgcct gggccaccga ctcgaattca ataattacca gccgcccggg cggcggcgat
     5401 gagccctcca gtacttcgag cgcgccaccg cgaaccagat agtgaccgcc gtagagccgc
     5461 accgcctgct cggcgagctg cttgtagacc tcgtaggcgc cagcgtcgcg cacatcggcg
     5521 cggacgatca gatagccttt tttcaaagct gcgactcgtc gaaagcggcc gtcttgacca
     5581 cccgatcaag cgccgccagc gtaccgagca ggctcgccat gtgtttcagc ggccaggcat
     5641 tcgggcc
//
LOCUS       DATDAA010000195         4769 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_503798,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000195 DATDAA010000000
VERSION     DATDAA010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503798"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="VIS73_06355"
     CDS             complement(<1..1318)
                     /locus_tag="VIS73_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5762810.1"
                     /translation="MSKPTRSERLRQWLGQRREGTEEAANADQRPVPAGASRRQRLHQ
                     WLLERRNAVWGPGAGTEGGTDQHTGRAGASWRQSLQIWWWKRRDWLVELIEGPAKSSE
                     DRKLAAAQLPGWDEWHGRMKDFPTSYKRCFLDTPLTTDDRFIRREKACDQIESTVERW
                     LAGGRESIAVIGEPGSGKSTLLNWITRHLGGEAPIKRTRFAQRLRRPEELCEILGAAL
                     GLSAKVDEQGLIDHLNEGTREVIIIDDAGRIGLRAPGGGEVVRCFLRIVLATQENVLW
                     VIGIRLPAWERFDDLYSIGRHFTTAIRLNYFARSELSEALRRRMEGAQLPMQVHHEEE
                     RSRKGEAADEREARVMNSWLAEVHKFSGGQFPLALYYWALVAEYDEEKKEVAIEFDRP
                     ATLDPEIPRGVQDLLTLAEIVVHGWLTVGEHAEIFQGKLDDSRHLLD"
     gene            complement(1315..2973)
                     /gene="gpmI"
                     /locus_tag="VIS73_06360"
     CDS             complement(1315..2973)
                     /gene="gpmI"
                     /locus_tag="VIS73_06360"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEY5762811.1"
                     /translation="MLKKLPDFPGVSGPVVLIVLDGYGLPRNAEGSAIAAARKPTLDA
                     LFANFPHMQLRAHGTAVGMPSDADMGNSEVGHNAIGAGQVYAQGASLVADAIASGAIW
                     NGKGWQEIVGPAKQGGTLHFIGLFSDGNVHSHIDHLKAMIARAKVEGVGRVRVHVLLD
                     GRDVPETSALEYLGPFEGFLADVRDSGFDVRIASGGGRQCITMDRYEANWLMVEKGWQ
                     THVLGEGQQFASAVEAVETLRKCHPGTIDQDLPPFVIAEAGKPVGVISDGDAVIFFNF
                     RGDRAIEITRAFEEADFDRFDRRRVPKVSYAGMLQYDGDLHLPKRFLVDPPAIRDTMG
                     ECFATLGIAQFACSETQKFGHVTYFWNGNRSGKFDGETWQEVPSDVVPFEQRPWMKAA
                     EITDAMIAAVQSGRYRVLRCNFANGDMVGHTGHFRAATMAIEAVDLALSRLLPAIAAA
                     GGVALITADHGNADEMYELDKKSGKPKCNADGSPKAKTAHTLNPVPLILYDRATEGRL
                     GLSALPTAGLSNLAATVANLIGFDKHPSWDDSLLTVSPTGNATQ"
     gene            complement(3110..>4769)
                     /locus_tag="VIS73_06365"
     CDS             complement(3110..>4769)
                     /locus_tag="VIS73_06365"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEY5762812.1"
                     /translation="VDHASGKVTALKNGVVIAESQLKTLVILDEVRAGGRIPLIIGRT
                     LTGRAREALGLPASEVFRQPEAPADSGRGYSLAQKMVGRACGVEGVRPGAYCEPKMTT
                     VGSQDTTGPMTRDELKELACLGFSSDLVMQSFCHTAAYPKPVDVRTHRELPAFISSRD
                     GVALRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPL
                     DMPESVLVRFKGKLQDGVTLRDLVNAIPYYAIKQGLLTVEKKGKKNIFSGRILEIEGL
                     PDLKVEQAFELSDAAAERSAAACAVQLNKEPIIEYMRSNITLMKWMIANGYEDKRTLG
                     RRIKAMEAWIAKPELLKADKDAQYAAVIEIDLADVKEPIVACPNDPDDVKLLSEVAGN
                     KIDEVFIGSCMTNIGHFRAAGKVLEGKSDIPTRLWVAPPTKMDAMILNEEGYYAVLGK
                     SGARMEMPGCSLCMGNQAQIKKGSTAMSTSTRNFPNRLGIDTFVYLGSAELAAICALM
                     GKIPTVAEYMEGVKVVNAKAADVYRYMNFDQIKEFSEVADTVTV"
BASE COUNT          812 a         1541 c         1529 g          887 t
ORIGIN      
        1 tgtcgagcag atgacggctg tcgtcgagct ttccctgaaa tatctccgca tgctcgccga
       61 cagtcagcca accgtgaacg acgatttcgg ccagcgtcag caggtcctga acgccacggg
      121 ggatctccgg gtccagcgtc gccggtcgat cgaactcgat ggcgacttcc ttcttctctt
      181 cgtcatactc ggcaaccagt gcccagtagt agagcgccag cgggaactgt ccgccgctga
      241 acttgtggac ttctgccagc caggagttca tcacccgagc ctcgcgctca tcggccgcct
      301 ctcccttgcg cgaccgctct tcttcgtggt gaacctgcat cggcagttgc gcgccttcca
      361 tgcgtcgacg caacgcctcg ctcaattcgc tgcgggcgaa atagttgagg cgaatcgccg
      421 tcgtgaaatg gcggccgatg ctgtaaagat catcgaagcg ctcccacgcc ggcagacgga
      481 tgccgatgac ccacagcacg ttctcctgcg tcgccagcac gatgcgcaag aaacaacgaa
      541 ccacctcgcc gccgccgggc gcgcgaagcc cgattcgccc ggcgtcatcg atgatgatta
      601 cctcgcgcgt gccctcgttg aggtggtcga tcaagccctg ttcatcgact tttgcggata
      661 gcccaagtgc cgcgccgagg atttcgcaga gttcttcggg ccggcgcaat cgttgcgcga
      721 atcgcgtgcg cttgattggc gcctcgccac cgaggtggcg cgtaatccag ttgagcagcg
      781 tcgacttgcc ggaccccggt tcgccgatga ccgcgatgct ctcgcgcccg ccagccaacc
      841 agcgctcgac ggtcgactcg atctggtcgc aggccttctc acgacggatg aaacgatcgt
      901 ccgtcgtcag cggggtgtcg agaaagcagc gcttgtagct ggtcgggaaa tccttcatgc
      961 gaccgtgcca ctcgtcccaa cccggcagtt gcgccgccgc gagtttgcgg tcctcgctgg
     1021 atttcgccgg tccctcgatg agttcgacaa gccaatcgcg ccgcttccac caccagatct
     1081 gcagcgactg ccgccaggat gcaccggcgc gtccagtgtg ctgatcggtg cctccctcgg
     1141 ttccggcgcc aggcccccag actgcattgc gccgctccaa gagccactga tgcagccttt
     1201 gccgccggct agcgccggca ggaaccggcc gctggtcggc gtttgctgcc tcttccgtac
     1261 cctcgcggcg ctgaccgagc cattgccgca accgttcgct gcgcgtcggc ttgctcattg
     1321 cgtcgcattt cccgttggcg agacggtcag cagactgtcg tcccacgacg gatgcttgtc
     1381 gaagccgatc agattggcga cggtggcagc aagattcgag aggccggccg tcggcagggc
     1441 agacaacccg agccggcctt cggtggcacg atcgtagagg atcagcggga ccggattcag
     1501 cgtatgcgcg gtcttggcct tgggcgatcc atcggcgttg cactttggct tgccactctt
     1561 cttgtcgagt tcgtacatct cgtcggcgtt gccgtggtcg gcagtgatca aggccacgcc
     1621 accggctgcc gcaatcgccg gcagcaggcg cgacaacgcg aggtcgaccg cttcgatcgc
     1681 catcgtcgcc gcacggaaat gtccggtgtg accgaccatg tcgccgttgg cgaaattgca
     1741 gcggagtacc cgatagcgac cgctctgcac ggctgcgatc atcgcgtcgg tgatctcggc
     1801 ggccttcatc cacgggcgct gctcgaaggg cacgacgtcg ctcggcactt cctgccaggt
     1861 ttctccatcg aacttgcccg agcggttgcc gttccagaaa taggtaacgt ggccgaactt
     1921 ctgcgtctcc gaacaggcga actgcgcgat gccgagcgtc gcaaagcact cgcccatcgt
     1981 gtcgcggatc gccggcgggt cgaccagaaa acgctttggc aggtgcagat cgccgtcgta
     2041 ctgcagcatg ccagcgtagc tcaccttcgg tacgcgccgg cgatcgaagc gatcgaagtc
     2101 ggcctcctcg aaggcgcgcg tgatttcgat cgcacggtcg ccgcggaagt taaagaagat
     2161 cacggcatca ccgtcggaaa taacgccgac gggtttgccg gcctcggcga tcacgaaggg
     2221 cggcagatcc tggtcgatgg tgccggggtg gcacttgcgc aacgtctcga cggcctcgac
     2281 cgcgctggcg aactgctggc cctcgccaag cacgtgcgtc tgccagccct tttcgaccat
     2341 cagccagttg gcctcgtagc gatccatcgt gatgcactgc cgaccgccgc cggaggcgat
     2401 gcgaacgtcg aagccgctgt cgcgcacgtc ggcaagaaag ccttcgaagg gtccgagata
     2461 ttcgagcgcc gaggtctccg gcacgtctcg cccgtcgagc agcacgtgaa cgcgtacgcg
     2521 gccgacgccc tcgactttgg cccgagcgat catcgccttg aggtggtcga tgtggctgtg
     2581 gacgttgcca tcggagaaca ggccgatgaa gtgcagcgtg ccgccctgct tcgctggccc
     2641 gacgatttcc tgccagccct ttccgttcca gatcgcgccg ctagcgatcg cgtcggcaac
     2701 gagtgatgca ccctgcgcgt agacctgccc ggcgccgatc gcgttgtgac cgacctcgga
     2761 attgcccatg tccgcatcgg acggcatgcc gactgccgtg ccgtgggcac gcagttgcat
     2821 gtgcggaaaa ttggcgaaca aagcgtcgag ggtcggcttg cgcgcggccg cgatggcgct
     2881 gccttcggcg ttgcgtggca gaccgtagcc gtcaagcacg atcaggacga ccggcccgga
     2941 cacgccgggg aagtcgggta atttttttaa catcgttggc gtttcggcaa tggaggcaat
     3001 gggtatcagg caaccgaccg gagacgcatt ctagcgggcg aaacctgccc gcctgcaccg
     3061 ggaagaaaaa cggcgccccg gagggcgccg cgatggcgtg gcaagcggat cagacggtca
     3121 ccgtgtcggc gacttcgctg aactccttga tctgatcgaa gttcatgtag cgatagacgt
     3181 cggctgcctt ggcattgacc accttgacgc cttccatgta ctcggcaacg gtcggaatct
     3241 tgcccatcag cgcgcagatt gccgccagct cggccgaacc aagatagacg aaggtatcga
     3301 tgcccagccg gttggggaag ttgcgcgtcg aggtcgacat cgccgtcgag cccttcttga
     3361 tctgcgcctg gttgcccatg cacagtgaac agccaggcat ttccatacgc gcacccgact
     3421 tgccgagaac ggcgtaatag ccctcctcgt tgaggatcat cgcgtccatc ttggtcggcg
     3481 gagcaaccca caggcgggtc gggatgtccg acttgccttc gagcaccttg ccagcggccc
     3541 ggaagtgacc gatgttggtc atgcagctgc cgatgaagac ttcatcgatc ttgttgccgg
     3601 caacttcgga gagcagcttg acgtcgtccg ggtcgttcgg gcaggcaacg atcggctcct
     3661 tgacgtcagc cagatcgatc tcgatcaccg cggcgtactg ggcatccttg tcggccttga
     3721 gcaactccgg cttggcgatc caggcttcca tcgccttgat gcggcggccg agcgtgcgct
     3781 tgtcttcata gccattggcg atcatccact tcatcagcgt gatgttcgag cgcatgtact
     3841 cgatgatcgg ttccttgttc agctgcaccg cacaggcggc ggccgagcgc tcggcagcgg
     3901 cgtcagagag ttcgaaggct tgttcgactt tgaggtccgg caggccttca atttcgagga
     3961 tgcggccgga gaaaatgttc ttcttgccct tcttctcgac ggtcagcagg ccctgcttga
     4021 tcgcgtagta cggaatcgcg ttgaccagat cgcgcagtgt gacgccgtcc tgcagcttgc
     4081 ccttgaagcg aacgagcacc gattccggca tatcgagcgg catgacgccg gtggccgccg
     4141 cgaaggcgac gaggccggag ccggccggga acgagatgcc gatcgggaaa cgggtgtgcg
     4201 agtcgccgcc ggtgccgacg gtatcgggca gcagcaggcg gttcagccac gagtggatga
     4261 cgccgtcgcc cgggcgcagc gcgacgccgt cgcggctcga gatgaaggcc ggcagctcgc
     4321 ggtgcgtcct gacgtcgacc ggcttggggt aggccgcggt gtggcagaag ctctgcatca
     4381 ccaggtccga ggagaagccc aggcaggcca gctccttgag ctcgtcgcgg gtcatcgggc
     4441 cggtggtgtc ctgggagccg acggtggtca tcttcggctc gcagtaggcg ccagggcgga
     4501 cgccctcgac accacaggcg cggccgacca tcttctgcgc cagcgaatag ccacggccgc
     4561 tgtcggccgg cgcctcgggc tggcggaaga cctcggaggc cggcagaccg agcgcctcgc
     4621 gggcgcggcc ggtcagggtg cgaccgatga tcagcgggat gcggccaccg gcgcgcactt
     4681 cgtcgaggat caccagggtc ttcagctgcg actcggcgat cacgacgccg ttcttgagcg
     4741 cggtgacctt gccggaagcg tggtcgacc
//
LOCUS       DATDAA010000196        10295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_503843,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000196 DATDAA010000000
VERSION     DATDAA010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10295
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503843"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /gene="gmd"
                     /locus_tag="VIS73_06370"
     CDS             complement(<1..663)
                     /gene="gmd"
                     /locus_tag="VIS73_06370"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016979321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY5762813.1"
                     /translation="MSKVAFITGITGQDGAYLAEFLLRKGYIVHGLKRRSSLFNTDRI
                     DHLYQDPHVSNRNFVLHHGDLTDSTNLIRIIQQVQPDEIYNLAAMSHVAVSFETPEYT
                     ANADGIGTLRILEAIRLLGLEKKTRFYQASTSELYGLVQETPQKETTPFYPRSPYACA
                     KIYGYWITVNYREAYGIYACNGILFNHESPLRGETFVTRKITRALARIKLGLQDCLYL
                     GNL"
     gene            complement(660..2087)
                     /locus_tag="VIS73_06375"
     CDS             complement(660..2087)
                     /locus_tag="VIS73_06375"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEY5762814.1"
                     /translation="MNRTTGQLTPVLLCGGSGTRLWPLSRTHMPKQFLRLSGEYSLLQ
                     ETLRRLDGLAAGRVVVVAGDDHRFLLAEQLRELDADADILLEPCARNTAPAIAAAAHW
                     ALRSDPEAVLLVLPSDHLIADAAAFHRAVEAGLPHAQTGQLVTFGIVPTRAETGYGYI
                     RRGAPVAGGYAVAGFVEKPDAPTAETYLASGEYTWNSGMFLFRADAFLRELERQAPTI
                     AGAAADALAASQRDLDFVRLDKTAFAASTADSIDYAVMERAEGVVVIPLDAGWNDIGA
                     WDALLTIGVPDAAGNRTVGDVLSHDSKGCYARASSRLVALVGMNDTVVVETADAVLVA
                     DKGRAQEVKHIVAALKAAGRAETEFHTVVHRPWGSYEGIALGPRYQVKRIIVKPGRAL
                     SLQKHHHRAEHWVVVTGTARVTRGDDVLLLGENESTYIPLGTVHRLENPGRIDLEIVE
                     IQSGSYLGEDDIVRLEDVYGRDKGK"
     gene            complement(2084..2449)
                     /locus_tag="VIS73_06380"
     CDS             complement(2084..2449)
                     /locus_tag="VIS73_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family EPS-associated transcriptional
                     regulator"
                     /protein_id="HEY5762815.1"
                     /translation="MLADEVRYRLLRELDANPAASQRDLARGLGVSVGKLNYCLRALV
                     DKGFVKVNNFRQSDNKLAYAYLLTPRGIEEKARVTVAFLQYKLAEYAQIEREIAELRA
                     EAANLDGTAVSPETQQEAA"
     gene            complement(2569..3030)
                     /locus_tag="VIS73_06385"
     CDS             complement(2569..3030)
                     /locus_tag="VIS73_06385"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY5762816.1"
                     /translation="MPEAAHRPADADYAARVQASFARQRAMATLGISLLRVEPGEVEL
                     EMPYNIDFCQQHGFLHAGTVTTAIDSACGYAAYSLMPADAEVLSIEFKINLLAPAKGE
                     RFRFIGRVVKAGRTISNVRGEAFASEPGRERLIATMDGTMMTLRERGAAAG"
     gene            complement(3023..4753)
                     /gene="ptsP"
                     /locus_tag="VIS73_06390"
     CDS             complement(3023..4753)
                     /gene="ptsP"
                     /locus_tag="VIS73_06390"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY5762817.1"
                     /translation="MSFTLHGLGVSNGIAIGRAHLLSYASLEVAHLTIPARLVDKELA
                     RFDVAVETVRAELDQLKCNTETVAAEVAAFVELHEMILADPALSAVPRDIIRERRCNA
                     EWAVVQQVNSLVAEFEAFEDPYLRERQADVRQVAERLIKELAGDAGATRVKSPKGVTE
                     AETVLVAHDLSPADVVAFKTRKFAGFITDVGGATSHTAILARSMALPAVVGLHEARTM
                     IREGEAIIVDGYRGVVIVNPDPRILEEYRLRQGQLQLERAKLKRLKTAKSATLDGVAV
                     GLYANIELPSDSAAAVEAGAEGVGLFRTEFLYLNRRDMPTEDEQFEAYRQVVREMDGR
                     PVTIRSFDLGADKQLDRGERTPTNPALGMRAIRISLAEPALFQSQLRAILRASRYGKV
                     KLLIPMLAHAHEIDATLAAVERAKSSLRGESVAFDEEIEVGGMIEIPAAALAIGLFIR
                     RLKFLSIGTNDLIQYTLAIDRSDDAVASLYDPLHPAVLGLIAHTIFSGEKSGIPVSLC
                     GELAGDPNLTRLLLGMGLRHFSMHASQILEVKQQVLRSDTNELGMLVRRLLRIEEPAK
                     LREQLDKLNA"
     gene            complement(4860..5129)
                     /locus_tag="VIS73_06395"
     CDS             complement(4860..5129)
                     /locus_tag="VIS73_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY5762818.1"
                     /translation="MPASEVEIVNKLGLHARASAKLTQTASKFRCEVWLERAGRRVNA
                     KSIMGVMMLAAAKGATITVETQGPDADDAMHELLALIADRFGEGE"
     gene            complement(5191..5586)
                     /locus_tag="VIS73_06400"
     CDS             complement(5191..5586)
                     /locus_tag="VIS73_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEY5762819.1"
                     /translation="MTIGILLVTHGQFGHSLVQNAADVLNRELTQVAALGLSPQDDPR
                     DLMPQATRLLASVDAGDGVLVLTDIWGASPANLATRLLAGGSVEGVAGVNLPMLLRAL
                     TYREKGMPTLLNKTVSGGRDGVLKMHRGG"
     gene            complement(5705..6742)
                     /locus_tag="VIS73_06405"
     CDS             complement(5705..6742)
                     /locus_tag="VIS73_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY5762820.1"
                     /translation="MHRLLRVAATLLGTLSLVACQQGSVVVQESFVFGTRVEVTVAGL
                     PEGEARAGAALVLAEFDRLHRMLHAWQPSEVTALNEAIAAGRARPVSPELGAILRDAQ
                     ALTLAGERLFDPGIGRLVALWGFHGDEFRPALPEPTALAAWRAARPSLADLRFDGDIV
                     RSVNPQVAVDLGGYAKGYALDRAAAILRHAGIANALVNIGGNVMALGDKNGKPWRVGI
                     RDPRGTGFIAALDLADGEAIGTSGDYQRFFEVDGQRYAHLLDPRSGTPAAGTRALTVL
                     VGSGPQAGTRSDVWSKPLFVADEAEFRNLADRLGAAQVLRVDADGRIQVASALAKRLR
                     WSDPPPPFAVY"
     gene            complement(6745..7701)
                     /gene="gshB"
                     /locus_tag="VIS73_06410"
     CDS             complement(6745..7701)
                     /gene="gshB"
                     /locus_tag="VIS73_06410"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY5762821.1"
                     /translation="MSLDLAFWIDPPETLKPAKDSSIAMMRAAAARGHRISVIGPQGL
                     AHVGGRVQGMARMLTLGGVGDDWCSLAAPERRPLRDFDAVLARKDPPFDIEYITSTWL
                     LQAAEREGARVVNAPRALREHSEKLAILEFPEFTVPGLVCRDGADLQAFIDAERDTVL
                     KPLDGMGGASVFRVRADDPNRNVIVETLGADGARTVFAQRYIPEITAGDKRILIVAGE
                     PLPYALARVPKAGETRGNLAAGGTGIAQPLSARDREIATEVGSRLAPRGLFLIGIDVI
                     GDWLTEINVTSPTCFVEISAQTELDVATRFIEALETHCATAR"
     gene            complement(7715..9016)
                     /gene="gshA"
                     /locus_tag="VIS73_06415"
     CDS             complement(7715..9016)
                     /gene="gshA"
                     /locus_tag="VIS73_06415"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY5762822.1"
                     /translation="MVPNLRTALSGPLQELERRFLESNVAIERWFRAKWGEHTPPFYG
                     SVDLRNAGFKLAPVDMNLFPGGFNNLNPDFLPLCVQATMSAIEKICPEAKGVLLIPEN
                     HTRNQFYLMNVARIVDILRQTGLNVRVGSLLPEVVAPTAIALPDGHTLTLEPLVRNGM
                     RLGLAGFDPCAILLNNDLSAGIPPILRGIHEQYLLPPLHAGWAVRRKSRHFRLYDDVA
                     SEFAELIGIDPWWINPVFAVCGKINFQDRQGEECLAAGVDDLLARIADKYRLLGITEK
                     PYVVVKADAGTYGMGVMTVRDAAEVKGLNRRQRNKMAVVKEGLAVSEVLIQEGVPTLE
                     RVGEGVAEPVVYMIDRFVVGGFYRVHRERGHDENLNAPGMHFEPLAFETGCQSPQFDA
                     GPDAPPNRFYAYGVVARLALLAAAYELEDEPAVEAPAAAAA"
     gene            complement(9071..10276)
                     /locus_tag="VIS73_06420"
     CDS             complement(9071..10276)
                     /locus_tag="VIS73_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5762823.1"
                     /translation="MNALGQIFTPAVVVEAMLALRRNQGSVLEPACGDGAFLRHLPGA
                     LGIEIDPAHCPSGARCDDFFALPRTARFATIIGNPPYVRFRDIPRATQRRLSGYGLNA
                     RANLYLYFIAKSLRHLEPGGEMIFITPRDFLKSTSAVPLNRELIAHGAFTDVVELGDI
                     RVFDEALPNCLIWRYQANPVSGPLRYAALRTDQALADSLAGLQWEERHCLELGGHLVF
                     SRQTYPYRVRDVAQVRVGAVSGADRIYVDTTHGNRDFVYSKTAATGRTRRMIWSEADP
                     LPPPVLMPHKAALLARRVAPFNETNWWRWGRSYPQSDAPRVYVNQRTRRAAPFFLHPC
                     QNFDGAILALFPRRADVELADFAAALNAVDWDDLGFFCDGRFIFAQRSLEEAPLPENF
                     AAFLPDGAG"
BASE COUNT         1703 a         3381 c         3539 g         1672 t
ORIGIN      
        1 caggttgccc aagtaaaggc aatcctgcag ccccagcttg atgcgcgcca atgcccgtgt
       61 gatcttgcgg gtgacaaagg tttcgccgcg cagcgggctc tcgtggttga agagaattcc
      121 attgcaggcg taaattccat aagcctcgcg gtaattgacg gtgatccagt agccgtagat
      181 cttggcgcag gcgtaggggc tgcgtggata aaacggagtg gtctccttct gcggtgtctc
      241 ttgtaccagg ccgtacagtt ccgaggtaga agcctgataa aagcgagtct tcttctcgag
      301 cccgagcaag cggatcgcct cgaggatgcg cagcgtgccg atgccgtcgg cgttggccgt
      361 gtactccggc gtctcgaagc tgaccgcgac gtgactcatc gccgccaagt tgtaaatctc
      421 gtcgggctgt acctgctgga tgatgcgaat caggttggtc gagtcggtga ggtcgccgtg
      481 gtgcaggacg aagttgcgat tgctgacgtg cgggtcctga tacaggtggt cgatgcggtc
      541 ggtgttgaac agcgacgaac ggcgcttcaa gccgtggacg atgtaacctt tcctcagcag
      601 gaattcggcg aggtaggcgc cgtcctggcc ggtgatgccg gtaatgaaag cgaccttgct
      661 catttgccct tgtcccttcc gtagacgtcc tcgagacgaa cgatgtcatc ctcaccgaga
      721 taactgcccg actggatctc gacgatctcg aggtcgattc gcccgggatt ttccagccga
      781 tgcacggtgc ctagcgggat gtaggttgat tcgttctcgc cgagcagcag gacatcgtcg
      841 ccgcgcgtaa cgcgcgcggt gccggtgacg acaacccagt gctcggcgcg gtggtggtgc
      901 ttctgcagcg acagcgctcg gccgggtttg acgatgattc gcttgacctg gtagcgcggg
      961 ccgagcgcga taccttcgta gctgccccag ggccgatgga cgaccgtgtg gaactcggtc
     1021 tcggcgcggc cggccgcctt gagcgcggcg acgatgtgct tgacctcctg ggcccgaccc
     1081 ttgtcggcga cgagcacggc gtcggcggtt tcgacgacca cggtgtcgtt catgccgacc
     1141 agggcgacga ggcgtgacga ggcgcgggcg tagcacccct tcgagtcgtg gctgagcacg
     1201 tcgccgacgg ttcggttgcc ggccgcatcg ggcacgccga tcgtcagcag cgcatcccag
     1261 gcgccgatat cgttccagcc ggcgtcgagc gggatcacca ccacgccctc ggctctttcc
     1321 atgaccgcgt agtcgatcga gtcggccgtg ctggcggcga aggcggtctt gtcgaggcga
     1381 acgaaatcga ggtcgcgctg cgacgctgcc aaggcgtcgg ccgccgcgcc ggcgatcgtc
     1441 ggcgcctgcc gttcgagttc gcgcaggaag gcgtcggcgc ggaacaggaa catgccgctg
     1501 ttccaggtgt attcgccact cgcaaggtag gtctcggcgg taggggcgtc gggtttctcg
     1561 acgaatcctg ccaccgcgta accaccggct accggggcgc cgcgacggat gtagccgtag
     1621 ccggtttcgg cgcgcgtcgg gacgatgccg aaggtgacca actgcccggt ctgcgcatgc
     1681 ggcaggcccg cttcgacggc gcgatggaag gccgctgcat cggcaatcag gtgatccgag
     1741 ggaagcacca gcagcaccgc ttcggggtcg ctgcgcagcg cccagtgggc ggcagcggcg
     1801 atggccggcg cggtgttacg tgcgcaaggc tcgagcagga tgtcggcgtc ggcatcgagt
     1861 tcacgcagtt gctcggcgag caggaagcga tggtcgtcgc cggcgaccac gacaacgcgc
     1921 ccagctgcca ggccatcgag gcgccgcaag gtttcctgca gcagcgaata ctccccggag
     1981 aggcgcagga actgcttggg catgtgggtg cgcgaaagcg gccacagccg cgtgcccgag
     2041 ccgccgcaga gcagcaccgg ggtcagctgt cctgttgttc gattcatgct gcctcctgtt
     2101 gggtttccgg cgaaaccgcg gtgccatcga gattggccgc ctcggctcgc agttcggcaa
     2161 tttcgcgctc gatctgcgcg tactcggcaa gtttgtactg aagaaaggcg acggtgaccc
     2221 gcgccttttc ctcgatgccg cgcggcgtca gcaggtaggc gtaagccagc ttgttgtcgc
     2281 tctggcggaa gttgttgacc ttgacgaaac ccttgtcgac cagcgcccgc agacaatagt
     2341 taagcttgcc gacgctgacg ccgagcccgc gggcaaggtc gcgctggctg gccgccgggt
     2401 tggcatccag ttcgcgcagc aggcggtagc ggacttcgtc ggcgagcatc ggtcgagagc
     2461 ggggcggccg acgggatcgg cgcgttcagg cgttgaacaa ggggcgcatt ctacagccgg
     2521 cggcgcaaaa ggcaatgccg cgggaatggc cggggtcagt cgcgtcgctc agccggccgc
     2581 agcgccacgc tcgcgcagcg tcatcatcgt gccgtccatc gtcgcgatca gccgctcgcg
     2641 cccaggctcg ctggcgaagg cctcgccgcg cacattgctg atcgtccgcc cggccttgac
     2701 gacacggccg atgaagcgga aacgctcgcc cttggccgga gccagcaggt tgatcttgaa
     2761 ctcgatcgac agcacttcag cgtcggccgg catcagcgag taggccgcgt aaccgcaggc
     2821 ggagtcgatc gccgtggtca ccgtgccggc gtgcagaaaa ccgtgctgct ggcagaagtc
     2881 gatgttgtag ggcatctcga gttcgacctc cccgggctcg acccgcagca gcgaaatccc
     2941 cagcgtcgcc atcgcccgct gtcgcgcaaa gctcgcctgg acgcgcgccg cgtagtcggc
     3001 gtcggccggc cggtgcgcgg cctcaggcat tgagcttgtc gagctgctcg cgcagtttcg
     3061 ccggctcttc gatccgcagc aggcggcgga ccagcatgcc gagttcgttg gtgtcgctgc
     3121 gcagcacctg ctgcttgacc tcgagaatct ggctggcatg catcgagaaa tgccgcaatc
     3181 ccatgccgag cagcaggcgc gtcaggttgg ggtcgcccgc cagttcgccg cagagcgaga
     3241 ccgggattcc cgacttctcg cccgaaaaaa tcgtgtgcgc gatcagcccg agcacggccg
     3301 ggtgcagcgg atcgtagagg ctggccacgg catcgtcgct gcggtcgatc gccagcgtgt
     3361 actggatcag gtcgttggtg ccgatcgaga ggaacttgag gcggcggatg aagaggccga
     3421 tcgccagcgc cgcggctggg atctcgatca tgccgccgac ctcgatctcc tcgtcaaagg
     3481 ccaccgactc gccgcgcagg ctcgacttgg cgcgctcgac ggcagccagc gtcgcgtcta
     3541 tttcgtgggc gtgggcgagc atcgggatca gcagcttgac cttgccgtag cgggaggcgc
     3601 gcaggatcgc ccgcagctgc gactgaaaca gcgccggctc ggccagcgag atgcggatcg
     3661 cccgcatgcc cagcgccgga ttggtcggcg tccgctcgcc gcggtcgagc tgcttgtcgg
     3721 cgccgaggtc gaaactgcgg atcgtcaccg gccggccatc catttcgcgg accacctgcc
     3781 ggtaggcttc gaactgctcg tcctcggtcg gcatgtcgcg ccggttgagg tagaggaatt
     3841 cggtgcggaa cagccctaca ccctcggcgc cggcctcgac cgccgcggcg ctgtcggacg
     3901 gcagctcgat gttggcgtaa aggccgacgg caaccccgtc gagcgtggcg ctcttcgccg
     3961 tcttcagccg cttgagcttg gctcgctcga gctgcagctg gccctggcgc agccggtatt
     4021 cctcgaggat gcgcgggtcg ggattgacga tgacgacgcc acgatagccg tcgacgatga
     4081 tcgcctcgcc ttcgcggatc atcgtccgcg cctcgtgcaa accgaccacg gccggcagcg
     4141 ccatgctgcg cgccaggatc gccgtgtgcg aagtggcgcc accgacgtcg gtgatgaagc
     4201 cggcgaactt gcgcgtcttg aaggcaacga cgtcggccgg cgacagatcg tgggcgacca
     4261 gcaccgtttc ggcctcggtc acgcccttgg gcgacttgac ccgagtcgcg cccgcatcgc
     4321 cggccagctc cttgatcagg cgctcggcga cctgacggac atcggcctgc cgttcacgca
     4381 ggtagggatc ttcgaaggcc tcgaactcgg cgaccagcga attgacctgc tgcaccacgg
     4441 cccactcggc gttgcagcgg cgctcgcgga taatgtcgcg gggcacggcc gagagcgccg
     4501 ggtcggcgag aatcatctcg tgcagctcga cgaaggccgc gacctcggcg gcaaccgtct
     4561 cggtgttgca cttcagttgg tcgagctcgg cgcgcaccgt ttccaccgca acgtcgaaac
     4621 gcgccagttc cttgtcgacc aggcgcgccg gaatcgtcag gtgcgcgact tccagcgagg
     4681 cataggagag caggtgcgcg cggccaatgg cgatgccgtt ggagaccccg aggccgtgca
     4741 gcgtgaagct catgccgata ccccgaaatg ccggcaatcc agctggcgcc agctagcccc
     4801 ccccttccca gggaaggggg caagggggat ggtcgtccac ccaaaggtag tcgccagcct
     4861 cattcgccct caccaaaacg gtcggcgatc agtgccagca gttcgtgcat tgcgtcgtcg
     4921 gcatccggcc cctgcgtctc gaccgtgatc gtcgccccct tcgccgcggc gagcatcatc
     4981 acgcccatga tgctcttagc gttgacgcgg cggccggccc gctcgagcca gacctcgcag
     5041 cggaacttcg acgccgtctg cgtgagcttc gccgaagccc gcgcatgcag gcccagcttg
     5101 ttgacgatct cgacttcgct cgcgggcaat tcaggtctcc gttgtcgccg gtagcggcag
     5161 tattcgggca ggatgcgtgc ggtccacgcg ctagcccccc cggtgcatct tgagtacgcc
     5221 gtcgcggcca cccgagacgg tcttgttgag cagtgtcggc atacccttct cgcggtaggt
     5281 cagcgcccgc aagagcatcg gcaggttgac gccggcaacg ccctcgacgc ttccgccggc
     5341 cagcaggcgc gtcgccagat tggccgggct cgcgccccag atgtcggtga gcaccagcac
     5401 accgtcaccg gcatcgacgc tggccagcag gcgcgtcgcc tggggcatca ggtcacgcgg
     5461 atcatcctgc ggcgagaggc cgagcgcggc gacctgcgtc agctcgcggt tgagcacgtc
     5521 tgcagcgttc tgcaccaagc tgtggccgaa ctggccgtgc gtgacgagca ggattccaat
     5581 ggtcattggc ttcgctttgc gccggcaccg ggttgcagcg gaatcgcctg cgcccgggca
     5641 gtcggcggag aagggcaaag cggcattcta aacaggcggt ccccgccgcg ccatcgcggc
     5701 gcgttcaata gacggcaaag ggcggcggcg ggtcgctcca gcgcaagcgc ttggccagcg
     5761 ctgacgcgac ctggatgcgg ccatcggcat cgacgcgcag gacctgcgcg gcgccaaggc
     5821 gatcggccag attccgaaat tccgcctcgt cggcgacgaa cagcggcttc gaccagacgt
     5881 ccgaccgggt gccggcctgc gggccgctgc ctaccagcac ggtcagcgcg cgggtgccgg
     5941 cggccggggt tccgctgcgc gggtcgagca ggtgcgcgta gcgctggcca tcgacctcga
     6001 agaagcgctg gtagtcgccc gaagtgccga tcgcctcgcc gtcggcaaga tcgagcgcgg
     6061 cgatgaaccc ggtaccgcgc gggtcgcgga tgccgacgcg ccagggcttg ccattcttgt
     6121 cgccgagcgc catcacgttg ccaccgatgt tgaccagcgc gttggcgatg cccgcgtggc
     6181 gcagaatcgc agcagcacgg tcgagcgcgt agcccttggc gtagccaccg agatcgaccg
     6241 ccacctgcgg gttcacgctg cgaacgatat cgccatcgaa gcgcagatcg gccaaagacg
     6301 gccgcgcggc gcgccaggcg gcgagcgcgg tcggctcggg cagtgccggc cgaaactcgt
     6361 cgccatggaa gccccagagc gccaccagcc ggccgatgcc aggatcgaac aggcgctcgc
     6421 cggccagcgt cagcgcctgc gcgtcgcgca ggatcgcgcc aagctcggga gacaccggtc
     6481 gggctcggcc ggcggcgatc gcctcgttca gcgccgtcac ttccgagggc tgccaggcgt
     6541 gcagcatgcg gtgcaaccga tcgaattcgg cgagcaccag cgccgcaccg gcgcgcgcct
     6601 cgccctcggg cagtccggcg acggtgacct ccacgcgggt gccgaagacg aagctctcct
     6661 gcaccaccac cgagccctgc tggcaggcaa ccagcgacag ggtgccgagc agcgttgccg
     6721 cgacgcgcaa cagcctgtgc atgctcagcg cgcagtggcg caatgcgttt cgagcgcctc
     6781 aataaagcgc gtcgcgacat cgagctcggt ctgcgcgctg atctcgacga agcaggtcgg
     6841 gctggtgacg ttgatctcgg tcagccagtc gccgatgacg tcgatgccga tcaggaacag
     6901 gccccgcggc gccagccggc tgccgacctc ggtagcaatc tcccggtcgc gtgcgctgag
     6961 cggctgtgca atgccggtgc cgccagcggc gagattgcca cgcgtctcgc cggccttagg
     7021 cacgcgtgcc agcgcgtagg gcagcggttc gccggcgacg atcaggatgc gcttgtcgcc
     7081 ggcggtaatc tcgggaatgt accgctgcgc aaaaaccgtt cgcgcaccgt ctgcgccgag
     7141 cgtctcgacg atcacgttgc ggttgggatc gtcggcgcgg acccggaaca ccgaagcgcc
     7201 gcccatgccg tcgagcggct tcagcacggt gtcgcgctcg gcgtcaatga aggcctgcag
     7261 gtcggcgcca tcgcggcaga ccagcccggg caccgtgaac tcgggaaact caagaatggc
     7321 aagcttctcc gaatgctcgc gcagcgcccg cggcgcattg acgacgcgcg caccttcgcg
     7381 ttcggccgcc tgcagcagcc aggtcgacgt gatgtactca atgtcgaagg gcgggtcctt
     7441 gcgcgcgagc acggcatcga aatcgcgcag cgggcgccgt tccggcgcgg ccagcgaaca
     7501 ccagtcgtct cccactccac ccagcgtcag catgcgcgcc attccctgca cgcgaccgcc
     7561 cacatgcgcc agcccctgcg ggccgatcac cgagatccgg tgaccgcgcg cggcggcggc
     7621 gcgcatcatc gcgatgctcg aatccttggc cggcttaagc gtctccggcg gatcgatcca
     7681 gaaggcgagg tcgaggctca cgctgccgcc tccctcacgc cgccgccgcg gccggcgctt
     7741 cgaccgccgg ctcgtcctcc agctcgtagg ccgccgccaa cagagccagc cgggcgacca
     7801 cgccgtaggc gtagaaacgg tttggcggcg catcgggacc ggcgtcgaac tgcgggctct
     7861 ggcagccggt ctcgaaggcc agtggctcga aatgcatgcc cggcgcgttg aggttctcgt
     7921 cgtggccacg ctcgcggtgc acacggtaaa agccgccgac gacgaagcgg tcgatcatgt
     7981 agaccaccgg ttcggcaaca ccttcgccga cgcgctcgag cgtcggcacg ccttcctgga
     8041 tcaacacctc gctcactgcc aagccttcct tgaccaccgc catcttgttg cgctggcggc
     8101 ggttgagccc cttgacctcg gccgcgtcgc gcacggtcat cacgcccatg ccgtaggtgc
     8161 cggcgtcggc cttgaccacg acgtagggct tctctgtgat gccgagcaga cggtacttgt
     8221 cggcgatgcg cgcgagcagg tcgtcgacac cggcagcgag gcattcctct ccctgccggt
     8281 cctggaaatt gatcttgccg cagaccgcga aaaccgggtt gatccaccac ggatcgatgc
     8341 cgatcagctc ggcgaactcg ctcgccacgt cgtcgtagag ccggaagtgc cgcgatttgc
     8401 gacgaaccgc ccagcccgcg tgcagcggcg gcagcaggta ttgctcgtgg ataccgcgca
     8461 agataggcgg aattcccgcc gacaggtcgt tgttgagcag gatcgcgcag ggatcgaatc
     8521 cggcgaggcc gaggcgcatg ccgttgcgca ccagcggctc gagcgtcagc gtgtggccgt
     8581 cgggcagggc gatcgcggtt ggcgccacga cctcgggcaa caggctgccg acgcggacgt
     8641 tgaggcctgt ctggcgcagg atgtcgacga tgcgcgcgac gttcatcagg tagaactggt
     8701 tgcgcgtgtg attctcgggg atcagcagga cacccttggc ctcgggacag atcttctcga
     8761 tcgccgacat cgtcgcctgc acgcaaagcg gcaggaaatc cgggttgagg ttgttgaagc
     8821 caccggggaa caggttcata tccaccggcg ccagcttgaa gccggcgttg cgcaggtcga
     8881 ccgaaccgta gaagggcggt gtgtgctcgc cccatttggc gcgaaaccaa cgctcgatcg
     8941 cgacgttcga ttcgaggaaa cggcgctcga gttcctgcag cgggccggac agggcggttc
     9001 ggaggttggg gaccattctc gggggccagg gttggttggg gtgtctcgca gctagtctaa
     9061 cgcgaccgac ctaccctgct ccgtccggca ggaaggctgc aaagttctcc ggcaacggcg
     9121 cctcttcgag actgcgctga gcgaagatga agcgcccgtc gcagaaaaac cccagatcgt
     9181 cccagtcgac ggcgttcagc gccgcggcga agtccgcaag ttcgacatcg gcacgtcgtg
     9241 gaaagagtgc gagtatcgcc ccgtcgaaat tctggcaggg atgcaggaag aaaggcgcgg
     9301 cgcggcgcgt gcgctggttc acatagacgc gcggcgcatc gctctgcggg taactgcgcc
     9361 cccagcgcca ccagtttgtt tcgttgaagg gagcgacacg gcgagcgagc agggcggcct
     9421 tgtgcggcat cagcaccggc ggcggcagcg ggtcagcctc gctccagatc atccgccgcg
     9481 tacggccggt ggcggcggtc ttcgaataga cgaagtcgcg gttgccatgc gtggtatcga
     9541 cgtagatgcg atcggcgccc gatactgccc cgacgcgaac ctgggccacg tcgcgcaccc
     9601 ggtacggata ggtctggcgg gagaaaacca ggtggccgcc gagttcgagg cagtgccgct
     9661 cctcccactg cagaccggcc agcgagtcgg cgagcgcctg gtcggtacgc agcgccgcgt
     9721 agcgcagcgg gccgctcacc ggattggcct gatagcgcca gatcaggcag ttgggcagcg
     9781 cttcgtcgaa gacccgaatg tcgccgagct caaccacgtc cgtgaaggcg ccgtgcgcaa
     9841 tcagctcgcg gttgagcggc acggccgatg tcgacttgag gaaatcgcgc ggcgtgatga
     9901 agatcatttc gccgcccggc tctaggtggc gcagcgactt ggcgatgaag tagaggtaga
     9961 gattggcacg ggcattcagc ccatagcccg acaggcgccg ctgcgttgcc cgcggaatat
    10021 cgcggaagcg cacgtagggc gggttgccga tgatggtggc aaagcgcgcc gtgcggggca
    10081 gggcgaaaaa gtcgtcgcaa cgcgcgccgg acgggcaatg cgcggggtcg atctcgatcc
    10141 ccagcgcgcc gggcagatgg cgcaggaagg cgccgtcgcc gcaggccggc tcgagcacgc
    10201 tgccctggtt gcggcgcaag gccagcatcg cctcgaccac cacggcgggc gtgaagatct
    10261 ggccgagtgc gttcacgtcg cggcgggcga aggca
//
LOCUS       DATDAA010000197         4854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_329285,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000197 DATDAA010000000
VERSION     DATDAA010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329285"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="VIS73_06425"
     CDS             <1..1308
                     /locus_tag="VIS73_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762824.1"
                     /translation="RRKRFSPPGDQVEIEQHQPDTLGRGGLLTIGDRRAQRRFGLVVA
                     VISKGNRSRLDRTGQRIDLFDNLDSGEIEIAEIILRWRRRLGRRFRRTRSRLCRLDRR
                     TARHLFVGFLEEGQRHRINGQTVELFSRPFYHFALQATSDIICARKLLYDAHALFPTT
                     RPKDDPDMPRNSVPFTTIRQLLASIALFAATAPAALAFQPLITDDTGTQGAGGNQLEA
                     GYSRVTIADDRENSISFAFTRGITDALDGFVETSRARSEPAGGPDSSGWSNVALGAKW
                     RFLECSECQYSLAFKPVVVLPVSAKIEADGLGTGKASWEATLIGTMEFGWGALHANAG
                     ISRTRFKDQVNGPDSDDWLVSLAPAVQLSERWLVAADFGVGESDAAGGSGTIELTFAE
                     IGTVFAPNEDLELALGVIRARGELAGVRAYATEVTAGVTRRFK"
     gene            complement(1301..2488)
                     /locus_tag="VIS73_06430"
     CDS             complement(1301..2488)
                     /locus_tag="VIS73_06430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEY5762825.1"
                     /translation="MSELILLPGKSRSLHRRHPWIFAGSVASLHGRARSGDTVVVVGD
                     DRKPLARAAWSPHSQIRARVWTFDPDESIDDAFFRRRVDAAFAARSAIPGLREQDGLR
                     LIHGESDGLPGVIADRYADIAVVQLTSAGAEKWRSAIVGALLRLPGIRRVYERSDSEV
                     RRLEGLEPVAGWREGEGGTEVEIAESGVRYGVDVAQGHKTGFYLDQRDNRSLLGRLAG
                     GRRVLNCFCYTGGFSLQALAGGAESVLSIDSSAPALTLARANLARNDALDAARAEWLA
                     DDVFEALRRFRADGRRFDLIVLDPPKFAPSAAHAQRAARAYKDINLLGFRLLNPGGLL
                     MSYSCSGGVGSELFQKIVAGAAADAGVNARIMARLGASADHPVALAFPEGEYLKGLLV
                     QVT"
     gene            complement(2485..3387)
                     /gene="xerC"
                     /locus_tag="VIS73_06435"
     CDS             complement(2485..3387)
                     /gene="xerC"
                     /locus_tag="VIS73_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY5762826.1"
                     /translation="MAESPSRIEAFLAALGTQRRMSALTLTAYRRDLEALQRLYGAEP
                     IDALDASQLRRAVSRMHAGGLSGRSLARRISAWRGYYRWLGERGLIAADPSGGLKPPK
                     SPKRLPKALSPDEAAKLLDGGEAPPGDQRLTLRDTAMFELLYSSGLRLAELAALAVAD
                     VEEIAAGELRVIGKRAKPRIVPVGTQARRAVVAWIQVRGDLAASGETALFVGARGRRL
                     SPRSIERRLGRLARLQGLEQHVHPHMLRHSFASHLLQSSGDLRAVQDMLGHASIASTQ
                     VYTHLDFQHLAKVYDAAHPRAKKK"
     gene            complement(3388..4047)
                     /locus_tag="VIS73_06440"
     CDS             complement(3388..4047)
                     /locus_tag="VIS73_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEY5762827.1"
                     /translation="MTLRADEVARYLQSNPQFFEEYSQLISQIVIPHPHGGRAISITE
                     RQMLALRNRNRELESKLSELIEFGEENDGISEKLHRLGMALVTAADLPVVLEALHHHL
                     TDDFSVPQVSLCLWDAPFAGELPVFVRIDDSLAVLAEPLVHPYCGDIATFDPAAWFGE
                     RAAGVRSQALMSLRHGDHTIGLLALGSEDPRRFYPEMGTLYLDRLAELTAAALVRVRG
                     Q"
     gene            complement(4058..>4854)
                     /gene="dapF"
                     /locus_tag="VIS73_06445"
     CDS             complement(4058..>4854)
                     /gene="dapF"
                     /locus_tag="VIS73_06445"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY5762828.1"
                     /translation="VVIDGVRQSVSLSADDVRRIADRHFGVGCDQLLLVEPSTVAGAD
                     FRYRIFNADGGEVEQCGNGARCFVRFVHDHGLSDRRELSVETMRGLIRPRLEADGSVS
                     VDMGVPRVAPEHVPFLVDDGQADAVVHRLAVGEETLLISVVSMGNPHAVTVVEDTESA
                     PVARLGPLIEAHRRFPGRVNAGFMAVVSRQHIRLRVYERGAGETLACGTGACAAAVSG
                     IRRGLLDSPVVVSTRGGDLTVSWAGDGAAVIMNGPASTVFEGELEL"
BASE COUNT          869 a         1642 c         1569 g          774 t
ORIGIN      
        1 cggcgcaaac gcttctcgcc gcccggcgat caggtagaaa tcgaacaaca tcagccagat
       61 acgctcggcc gcggcggcct gctcaccatc ggcgatcgcc gcgcgcagcg tcgcttcggt
      121 ctcgtcgtcg cggtcattag caaaggcaat cgcagccgcc tcgatcgcac cggccagcgg
      181 atcgacctct tcgacaacct cgattccggt gaaatcgaga tcgccgagat catcctcagg
      241 tggcggcggc ggcttggccg gcgcttccgg cgcacgcgca gccggctttg caggcttgac
      301 cggcgcacgg ctcggcatct tttcgtcgga tttcttgaag aagggcagcg ccatcggata
      361 aacggccaga cagtagaact attcagccgg cctttttatc atttcgccct ccaagcgaca
      421 agcgacatta tttgcgcgcg caagctactt tatgatgcgc atgcactatt tccaacgaca
      481 cgacccaagg acgaccccga catgccccgc aattccgtcc cattcacgac catccgccag
      541 ctcctagcaa gcatcgctct gttcgccgcc acggctcccg ccgcgctggc cttccagcct
      601 ttgattaccg acgataccgg cacccagggc gccggtggca atcagctcga agccgggtac
      661 tcacgggtca cgatcgccga tgaccgggaa aactcgatct cgttcgcgtt tacccgtggc
      721 atcaccgatg ccctggacgg cttcgtcgag acctcccgtg cccgaagcga gccggccggc
      781 ggacctgaca gcagcggctg gagcaacgtc gcgctgggcg ccaagtggcg tttccttgaa
      841 tgcagcgaat gccagtacag cctcgctttc aagccggtgg tcgttctccc ggtcagcgca
      901 aaaatcgagg cggacgggct gggcaccggc aaggcttcct gggaagcgac gctgatcggc
      961 acgatggaat tcggctgggg ggcgctacac gccaacgccg gaattagccg tacgcgtttc
     1021 aaggatcagg tcaatggccc ggactcggat gactggctgg tgtcgctggc gccggccgtt
     1081 caactcagtg agcggtggct ggtagccgcc gactttggtg tcggcgaaag cgatgcggcc
     1141 ggcggcagcg gcactatcga gttgaccttt gccgagatcg gcactgtgtt tgcaccgaat
     1201 gaggaccttg aactggccct tggcgtgatc cgcgcccgcg gcgagctcgc cggtgtgcgc
     1261 gcttacgcga ccgaggtcac cgccggtgta acccggcgtt tcaagtgacc tgaacgagca
     1321 ggcccttcag gtactcaccc tcgggaaacg caagcgcgac cggatggtcg gcgctggcgc
     1381 cgaggcgggc catgatgcgg gcgttgacgc cggcatcggc cgcggcgcca gcgacgatct
     1441 tctggaacag ttcgctgcca accccgcccg agcacgaata gctcatcaac agaccgcctg
     1501 ggttgagcag ccggaagcca agcaggttga tgtccttgta ggcccgcgcg gcgcgctgcg
     1561 catgagcagc agacggcgcg aacttcggcg ggtcgaggac gatcaggtcg aatcgccggc
     1621 cgtcggcgcg gaatcgtcgc agcgcctcga acacatcgtc ggccagccat tcggcccgcg
     1681 ccgcatctaa cgcgtcattg cgcgccagat tggcccgggc cagcgtcaag gctggcgccg
     1741 aggagtcgat cgagagcacg ctctcggcac cgccggccag cgcctgcagc gagaaaccgc
     1801 ccgtatagca gaagcagttg agcactcggc ggccacctgc caggcgtccc agcaaggagc
     1861 ggttgtcgcg ctgatcgaga tagaagccgg tcttgtgccc ctgtgcaaca tcaacgccat
     1921 agcgtacgcc ggattcggca atctccactt cggtgccgcc ctcgccctca cgccagccgg
     1981 cgacgggttc taggccttct aggcgccgga cttccgagtc ggagcgttcg tagacgcggc
     2041 gaatgccggg caggcgcagc agggcgccga cgatcgccga acgccatttc tcggcgccgg
     2101 cgcttgtcag ctgcacgacc gcgatgtcag cgtagcggtc ggcgatcacg ccgggcaggc
     2161 cgtccgactc gccgtggatc aggcgcagtc cgtcctgctc gcgcaggccg gggatggcag
     2221 atctcgcagc gaacgccgcg tcgacgcgac ggcggaaaaa ggcgtcgtcg atcgattcgt
     2281 cgggatcgaa agtccacacg cgcgcgcgga tctgcgaatg cgggctccac gccgcgcgcg
     2341 ccagcggctt gcgatcgtca ccaacgacca ccacggtgtc gccggaccgc gcccgtccgt
     2401 gcaggctggc caccgatcca gcaaagatcc acgggtgtcg ccgatgcagc gagcgggact
     2461 tgccgggcaa caggatcaat tcactcattt cttctttgct cgtgggtgcg ctgcgtcata
     2521 gaccttagcc agatgctgga aatcgaggtg ggtgtagacc tgcgtcgagg cgatactcgc
     2581 gtgccccagc atgtcctgca cggcacgcag gtcacccgat gactgcaaca gatgggaggc
     2641 aaaggaatgc cgcagcatgt gcgggtgcac gtgctgctcg agcccctgca agcgggcgag
     2701 gcgtccgaga cgccgctcga tgctgcgcgg cgaaagccgg cggccccggg caccgacgaa
     2761 cagcgcggtt tcacccgatg ccgccagatc cccgcgcact tggatccagg cgacgacggc
     2821 ccgacgcgcc tgcgtaccga ctggcacgat gcgcggcttg gcgcgcttgc caatcacgcg
     2881 taactcgccg gcggcgatct cctcgacgtc agcaacagcc aacgccgcca gttcggcaag
     2941 gcgcaatccc gacgagtaga gcagttcaaa cattgccgtg tcgcgcagcg tcaggcgctg
     3001 gtcgcccggc ggcgcctcgc cgccgtcgag cagtttcgcg gcttcgtcgg gcgacaacgc
     3061 cttgggtaga cgcttggggc tcttaggcgg tttgagtccg ccactggggt cggcggcgat
     3121 cagcccccgc tcgccaagcc aacggtagta cccgcgccag gccgagattc gccgggccag
     3181 cgatcggccc gacagcccgc ccgcgtgcat gcgcgacact gcgcggcgaa gctggctcgc
     3241 gtcaagcgcg tcgatcggct cggcgccgta aagtcgttgc agcgcttcga ggtcccgccg
     3301 ataggccgtg agcgtcagcg ccgacatgcg gcgctgtgtt ccgagtgccg cgagaaacgc
     3361 ttcgatccgc gacggcgact ccgccatcta ctgcccgcga acccggacca gcgccgctgc
     3421 cgtcagttcg gccaggcgat cgaggtagag cgttcccatt tccgggtaga agcgccgcgg
     3481 gtcttccgat cccagcgcca gcaggcctat cgtgtggtcg ccgtggcgaa gcgacatgag
     3541 tgcctgcgac cgcacgccgg ccgcgcgctc gccaaaccaa gccgccgggt cgaaggtggc
     3601 aatgtcgccg cagtaggggt gcaccagtgg ctcggcgaga acggcgagcg aatcgtcgat
     3661 gcgcacgaat accggcagct cgccggcaaa cggcgcatcc cagaggcaga gcgatacctg
     3721 cggcaccgag aaatcgtcgg tgaggtggtg atgcagcgct tccagcacca ccggcaggtc
     3781 tgcggcggtc accagcgcca tgcccagccg gtgcagcttc tcgctgatgc cgtcgttctc
     3841 ctcgccgaac tcgatcagct cggaaagctt gctttcgagt tcgcggttgc ggttacgcaa
     3901 ggccagcatc tgccgctcgg taatcgagat cgcgcgcccg ccatgcgggt gcggaatcac
     3961 gatctgcgaa atgagctgcg aatactcctc aaaaaactgc ggattgcttt gcaggtagcg
     4021 tgcgacctcg tctgcgcgca gcgtcatcga tttctcccta gagttccagt tcgccttcaa
     4081 atacggtgct tgccggaccg ttcatgatca cggccgcgcc gtcgccggcc cagctgaccg
     4141 tcagatcgcc gccgcgcgtc gataccacta ccgggctgtc gagcaggcca cgcctgatgc
     4201 ccgagacagc tgccgcgcag gcgccggtgc cgcaggccag cgtttcgccg gcaccgcgct
     4261 cgtagacgcg caggcgaatg tgctggcgcg aaacgaccgc catgaatccg gcattgaccc
     4321 gcccggggaa gcggcgatgc gcttcgatca gtggaccgag ccgtgcaacg ggcgcggatt
     4381 cggtgtcttc cacgacagtc acggcgtgcg gattgcccat cgaaacgacg ctgatcaaca
     4441 aggtctcctc gcccaccgcg aggcggtgca cgacggcgtc cgcctgcccg tcgtcgacca
     4501 gaaacggcac atgttcgggc gccacccgcg gaacgcccat gtcgacgctg acgctgccgt
     4561 cggcctcgag ccgcggccgg atcaggccgc gcatcgtctc gacgctcaat tcacgacgat
     4621 cggacaggcc atggtcgtgg acgaaacgca cgaagcagcg cgcaccattg ccgcattgct
     4681 cgacctcgcc gccgtcggca ttaaagatgc gatagcgaaa atcggcaccg gcgacggtcg
     4741 atggctcaac cagcagcagc tggtcgcagc cgacgccgaa atgccggtcg gcaatgcgcc
     4801 gcacgtcatc ggccgagagt gataccgact ggcgaacgcc gtcgatgacg acga
//
LOCUS       DATDAA010000198         2678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_434291,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000198 DATDAA010000000
VERSION     DATDAA010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434291"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..936)
                     /gene="istB"
                     /locus_tag="VIS73_06450"
     CDS             complement(163..936)
                     /gene="istB"
                     /locus_tag="VIS73_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEY5762829.1"
                     /translation="MSADALVTTLKSMKLHGMADAVAELAAQGAPAFRQSNELLAKLI
                     KAEVAEREVRSVNYQMHIARFPAHKDLAGFDFAQSTVSEGQVKALHHCEFLDDTENIV
                     LVGGPGTGKTHLATAIGIQAIIHHHRRVRFLSTVELVNALEQEKASGKAGRLANRMTH
                     VDLVILDELGYLPFSQAGGALLFHFLSKLYERTSLIITTNLSFAEWPAVFGDAKLTTA
                     LLDRLTHHCHILETGNDSYRLKNSTNNSKETKAKKRTKT"
     gene            complement(926..2443)
                     /gene="istA"
                     /locus_tag="VIS73_06455"
     CDS             complement(926..2443)
                     /gene="istA"
                     /locus_tag="VIS73_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEY5762830.1"
                     /translation="MDLLSVIRRWHHREGLAIREIKRRTGLSRNTIRKYLASDVVEPK
                     YPARKSASKLDPYAGVLASWLTRESKRNRKRRRNLKQLHRDLVALGFAGSYDRVAAFA
                     RDWRQRERERAKKASRGAFVPLTFAPGEAFQFDWSEDWLVIGAKKTKLQVAHTKLCHS
                     RAFYLRAYLTQTHEMLFDAHNHAFRVLGGVPERGIYDNMKTAVDKVGRGKERLVNARF
                     RAMVSHFLFESEFCNPAAGWEKGQVEKNVRDARHRLLQDAPAFADLAEANTWLEARCQ
                     ALWHTVTHPEQGHRTVAEVHAEERAALMAMPPPFDGFIELTKRVSPTCLVVFERNRYS
                     VPAAFANRVVSLRAYAARIVLVAEAEVIAEHPRVFSRDHSSKGVTIYDWRHYLAVVQR
                     KPGALRNGAPFTDLPPSFKRLQSVLMQRDGGDREMADILALVLHHDERLVEQAVREAI
                     ESDVISKTHILNRLSRLLDTPLPAAFTPPPALTLREEPTANTERYDALRGTRHVS"
BASE COUNT          478 a          816 c          839 g          545 t
ORIGIN      
        1 actgacggta atgacataac gacaaaactc tttgtgggaa gtcggatttc gggttatgcg
       61 ctacctgatt ttgacgaact aacttcagac tgttgatttg caccgaaagt tgacccggga
      121 tttgcgctga aaattgaccc acccggatgg ccagattatg gctcaggtct tggttctttt
      181 cttcgctttt gtctccttcg agttgttggt tgagtttttg aggcggtaac tgtcgttgcc
      241 ggtctcgagg atatggcagt ggtgcgtcag ccggtcgagt agcgccgttg tcagcttggc
      301 atcgccgaac acggcaggcc attcggcaaa gctcaggttc gtcgtgatga tgaggctggt
      361 tcgctcgtag agtttgctta ggaagtggaa caggagcgcg ccgccagcct ggctgaacgg
      421 caagtagccg agttcatcga ggatcacgag atcgacgtgg gtcatgcgat tggcaagtct
      481 gccggctttg ccgctcgcct tttcctgttc gagcgcgttg acgagctcga cggtggacag
      541 aaaccgcacg cgccggtgat ggtggatgat cgcttgtatg ccgatcgccg tggccaggtg
      601 cgttttgccg gtgccggggc caccgacgag gacgatgttc tcggtgtcat cgaggaactc
      661 gcagtggtgc agggctttta cctggccttc ggagaccgtg ctctgggcaa agtcgaagcc
      721 ggcgagatcc ttgtgggccg ggaaccgggc aatgtgcatc tggtagttga cggagcgcac
      781 ctcgcgctcg gcgacttcgg ccttgatcag cttcgcgaga agctcgttgg actgcctgaa
      841 ggccggcgcg ccctgcgcgg cgagctcagc caccgcatcc gccatgccgt ggagcttcat
      901 cgacttcaag gtcgtcacga gcgcatcagc tgacatgacg ggtacctctc agggcgtcgt
      961 aacgctcggt gttcgcggtc ggctcctcgc gcagcgtcag cgccggcggc ggtgtgaagg
     1021 cggccggcag tggtgtgtcg agcaagcggc tcaggcggtt caggatatgc gtcttggaga
     1081 tgacgtcgga ctcgatcgcc tcacgtaccg cctgctcgac gaggcgctcg tcgtggtgca
     1141 gcaccagcgc gaggatatca gccatctccc ggtcaccgcc gtcgcgctgc atcagcaccg
     1201 actgcagccg cttgaagctt ggcggcaggt cggtgaatgg ggcgccgttg cgaagggcgc
     1261 cgggtttacg ctgcacgacc gcgagatagt ggcgccagtc atagatcgtg acgcccttgc
     1321 tgctgtggtc gcggctgaac acacgcgggt gctctgcgat gacctcggcc tcagcgacca
     1381 gcacaatacg agcggcgtag gctctcagac tcacgacccg gttcgcgaac gctgccggca
     1441 cgctgtagcg gttgcgttcg aacaccacca gacaggtcgg cgagacgcgc ttggtcagct
     1501 cgataaaccc gtcgaacggc ggcggcatcg ccatcagtgc ggcccgctcc tcggcgtgga
     1561 cctcggcgac cgtgcgatgc ccctgctcgg ggtgggtgac cgtatgccac agcgcctggc
     1621 agcgcgcttc gagccaggtg ttggcctcgg cgaggtccgc gaaggccggg gcatcctgca
     1681 gcagccgatg gcgggcatcg cggacgttct tctcaacctg gcccttctcc cagccggcag
     1741 ccgggttaca aaactccgac tcgaacagga agtggctgac catggcccgg aagcgggcgt
     1801 tgaccaggcg ctccttgccg cggccgacct tgtcgaccgc cgtcttcatg ttgtcgtaga
     1861 tgccgcgctc aggcacgccg cccagcaccc gaaaagcgtg gttgtgggca tcgaacagca
     1921 tctcgtgggt ctgggtcagg taagcgcgca gataaaacgc ccggctgtgg caaagcttgg
     1981 tgtgcgcgac ctgcagcttc gtcttcttcg ctccgatgac gagccaatcc tcgctccagt
     2041 cgaactgaaa ggcctcgccc ggtgcaaagg tgagcggcac gaaggcaccg cggctggcct
     2101 tcttggcgcg ttcccgctcc cgctgccgcc agtcccgggc aaaggcggcc acccggtcat
     2161 aggaaccggc aaagcccagt gccacgagat cgcggtgcag ctgcttcagg ttccgccggc
     2221 gcttgcgatt gcgcttcgac tcccgggtta gccagctcgc caacaccccg gcatacggat
     2281 cgagcttgct cgcactcttc ctcgccggat acttcggctc gaccacgtcg ctcgccaggt
     2341 acttacggat cgtgttccgg gacagcccgg tccgtctctt gatctcccgg atcgccaacc
     2401 cttcacgatg gtgccagcgc cgaatcacac tcaataaatc cacgtctatc actcccaaaa
     2461 agctcccgcc atcctgcagc gggatgaagc gttacacgtg ggtcagtttt agatgcaaat
     2521 acccgggctt agtgggtcac ttttgggtgc aaatcaacag tacgttatct tgagctagtt
     2581 tctacatatg aaacgcagtt gcggtgtggg ctactcaaaa tcctccagcc aaaatttctc
     2641 aagtacccag cgccaaaccc accacgatac ggccatcc
//
LOCUS       DATDAA010000199         2684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_364810,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000199 DATDAA010000000
VERSION     DATDAA010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364810"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /gene="frr"
                     /locus_tag="VIS73_06460"
     CDS             <1..486
                     /gene="frr"
                     /locus_tag="VIS73_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY5762831.1"
                     /translation="GKVRTGRAHTGLLDHVMVDYYGNPTPVNQVANITLVDARTIGVQ
                     PWEKNMVGPVERAIRDSDLGLNPATNGELIRVPMPMLTEERRRELTKVVKSEGENAKV
                     AIRNLRRDANHGFKEAVKSKEISEDDDRRAQDEVQKLTDRFVQEIDKMLAQKEAELMQ
                     V"
     gene            507..1292
                     /gene="uppS"
                     /locus_tag="VIS73_06465"
     CDS             507..1292
                     /gene="uppS"
                     /locus_tag="VIS73_06465"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY5762832.1"
                     /translation="MADAAHASSTRTIPPVGAVPRHIAIIMDGNGRWARKRLLPRVAG
                     HRKGVEAVRETVSICREYGVEYLTLFAFSSENWRRPAEEVSFLMQLFMRALESEIDKL
                     DEAGVRLRVIGERSAFDKPLQELIGAAETRTAGNSRLQLTIAANYGGRWDILQAAQRL
                     AQSGATPPEQWTEAALSPHLSMAFAPEPDLFIRTGGEQRISNFLLWQLAYSELHFTDT
                     LWPEFGRGAFDAALASYRRRERRFGRTSEQLYAAAGDVRREAG"
     gene            1295..2116
                     /locus_tag="VIS73_06470"
     CDS             1295..2116
                     /locus_tag="VIS73_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY5762833.1"
                     /translation="MLRTRIITALVLAACLFAAVLSLQTLHWAWIVAGVAGLGGWEFG
                     RLAGWGKASPALGVWVFVLTAGVIVLHPATAGLAEFGASPLVLPMLGAAAAFWIVLVP
                     LWLKHRWPIGPLAGLVVGTIVLVPTWLAIVQLHRLHPWLLIAAMAFVSFADIAAYFSG
                     RRFGRRKLAPEISPGKTWEGVFGAFAATTVFGLGVAFAIGWADSALRLLSLIVLMPAL
                     TLISVAGDLFESMLKRRAGMKDSSQLLPGHGGILDRIDSLTAALPVAALALLLIP"
     gene            2126..>2684
                     /locus_tag="VIS73_06475"
     CDS             2126..>2684
                     /locus_tag="VIS73_06475"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929136.1"
                     /note="catalyzes the formation of 2-C-methyl-D-erythritol
                     4-phosphate from 1-deoxy-D-xylulose-5-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY5762834.1"
                     /translation="MRTLTVLGSTGSIGRSTLDVVRRHPQRFRIAALCAHRQVDALAD
                     QCLEFRPTLAVVGSAEGAAQLQAKLRSAGLATRVAFGPDALREAASLPEVDSVMAAIV
                     GAAGLMPTLAAVEAGKRVLLANKEALVMAGPIFVAAVREHGAVLLPIDSEHNAVFQAL
                     PTGYVGEPAAAGIRRILLTASGGPFR"
BASE COUNT          414 a          898 c          906 g          466 t
ORIGIN      
        1 ggcaaggtgc gcaccgggcg cgcgcatacc ggcctgctgg atcatgtgat ggtggattat
       61 tacggcaacc cgacgccggt gaaccaggtt gcgaacatca ccctggtcga cgcgcgcacc
      121 atcggcgtac agccgtggga aaagaacatg gtcggcccgg tggagcgcgc gatacgcgac
      181 tccgacctgg gcctgaaccc ggcaaccaat ggcgaactga tccgcgtgcc gatgccgatg
      241 ctgaccgagg aacgccgccg agaattgacc aaggtcgtga agagcgaagg cgagaacgcc
      301 aaggtcgcca tccgcaacct gcgccgcgac gccaatcacg gcttcaagga agccgtgaag
      361 tcgaaggaga tctcggaaga cgacgatcgc cgcgcgcaag acgaggtgca gaagctgacc
      421 gaccgtttcg tgcaggaaat cgacaagatg ctcgcccaga aagaggccga gctgatgcag
      481 gtctgagcga gagcgacgac cccccgatgg ccgacgcggc gcacgccagc tcgacccgga
      541 ccatcccgcc cgtgggcgcg gttccgcggc atatcgccat catcatggac ggtaacggtc
      601 gctgggcgcg caagcgtctg ctgccgcgcg tcgccgggca ccgcaagggt gtcgaggcgg
      661 tgcgcgaaac ggtgtcgatc tgccgtgagt acggcgtcga gtacctgacg ctgttcgcct
      721 tcagttccga gaactggcgg cgacccgccg aggaagtctc gttcctgatg cagctgttca
      781 tgcgcgcgct tgagagcgag atcgacaagc tcgacgaggc cggcgtgcgt ctgcgcgtga
      841 ttggcgaacg aagcgccttc gacaagccgc tgcaggagtt gatcggcgct gcggaaacgc
      901 gaaccgccgg gaacagccgt ttgcagctga cgattgccgc gaactacggc ggtcgctggg
      961 acatcctgca ggccgcgcag cgcctcgcgc aaagtggtgc cacgccgcct gagcagtgga
     1021 ccgaagcggc gctttcgccg catctgtcga tggccttcgc gccagaaccc gatctgttca
     1081 tccgcactgg cggggagcag cgcatcagca atttccttct gtggcagctc gcctattcgg
     1141 aactccattt caccgacacc ctgtggcccg agttcggtcg cggcgccttc gatgcggcac
     1201 tggcgtctta tcgccgccgc gagcgtcgct tcgggcgaac cagcgagcag ctttacgccg
     1261 cggcgggcga cgtcaggcgc gaggcaggct aagcgtgctg cggacgcgca tcatcactgc
     1321 gctggtgctc gctgcctgcc tctttgccgc agtgctttcc ctgcagacgc tgcactgggc
     1381 atggatcgtc gccggcgtcg ccggcctcgg cggctgggaa ttcgggcggc tagccggctg
     1441 gggcaaggca tcgccggcgc tcggcgtctg ggttttcgtg ctgactgccg gcgtcatcgt
     1501 gcttcacccg gcgacggcag gcctcgccga attcggcgct tcgccattgg tcttgccgat
     1561 gctgggtgcc gccgcggcat tctggatcgt gctggtgccg ctctggttga agcatcgctg
     1621 gccgatcgga ccgctggccg gcctggtggt cggcaccatc gtcctcgtac cgacctggct
     1681 ggcgatcgtc cagctgcacc ggctgcatcc ctggttgcta attgcggcga tggccttcgt
     1741 ttccttcgcc gacatcgccg cctacttctc cggtcggcgc ttcggccggc gcaagctggc
     1801 gcccgaaatc agcccgggca agacctggga aggcgttttc ggcgccttcg cagcgaccac
     1861 cgtcttcgga ctcggcgtag cctttgccat cggctgggcc gacagtgcgc tgcggctgct
     1921 gtcgctgatc gtgctgatgc cggcgctgac cttgatcagc gtcgccggcg acctcttcga
     1981 atcgatgctc aagcgccgcg ccgggatgaa ggacagtagc cagctcctgc ccggccacgg
     2041 tggcatcctc gatcgcatcg acagcctgac ggcggcactg ccggtcgccg cgcttgcctt
     2101 gctgctgatt ccctgagccg tggtcatgcg cacgcttacc gtactgggtt cgacgggttc
     2161 gatcggacgc agcacgctcg acgtcgttcg tcgccacccc caacgcttcc gcatcgccgc
     2221 gctctgcgcg catcggcaag tcgatgcgct ggcggaccaa tgccttgaat tccgcccgac
     2281 gctggccgtg gtcggctcgg cggaaggcgc tgcgcagctg caggcgaagc tgcgcagcgc
     2341 gggccttgcc acgcgggtgg ccttcggccc ggacgccctg cgcgaagctg catccttgcc
     2401 cgaggttgat tcggtgatgg ctgccatcgt cggggcggcg gggctgatgc cgacgctcgc
     2461 cgcggtggaa gccggcaagc gcgtgctgct cgccaacaag gaagcactgg tcatggccgg
     2521 tccgatcttc gttgcggccg tgcgtgaaca cggcgctgtc ctgttgccga tcgactccga
     2581 acacaacgcg gtgttccagg cgctgcccac cggatacgtc ggcgagccgg ccgccgcggg
     2641 cattcgccgc atcctgctga ccgcctcggg cggcccgttt cgcc
//
LOCUS       DATDAA010000200         2504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_84809,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000200 DATDAA010000000
VERSION     DATDAA010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84809"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VIS73_06480"
     CDS             complement(<1..597)
                     /locus_tag="VIS73_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762835.1"
                     /translation="MQGSDSFWGACLVSAAIVLSGSSAALAESTPGAGLEASVMVLRD
                     ALERVAAQRGQDRRTVLAEVDRLIGEAKGSRDQAPALLGQAYALVRAEIRLLAQEQNA
                     PRNEAPAASVRAPTRQSYERVRDSAGALRDALLRLSQEAGREGAATQVAAVDRLLVDA
                     ATAADGERWETAQGLATQAMDAARAGVRQIREGTTQVRA"
     gene            complement(633..2024)
                     /gene="cysS"
                     /locus_tag="VIS73_06485"
     CDS             complement(633..2024)
                     /gene="cysS"
                     /locus_tag="VIS73_06485"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY5762836.1"
                     /translation="MLRIYNTLSREKQEFVPLTPGQVRMYVCGMTVYDLCHLGHARVM
                     VVFDMVYRWLRASGYAVDYVRNITDIDDKIIRRAGENAESIRALTERYIGLMHEDADA
                     LGVLRPDHEPRATDYVPQMLAMISELETRGFAYRADDGDVNFAVRCFEPYGRLSGKSI
                     DDLRAGERVEVDSAKRDPLDFVLWKHAKAEEPAEAKWASPWGEGRPGWHIECSAMSSC
                     LLGEHFDIHGGGMDLQFPHHENEIAQSEAANGKTFVNYWMHNGFVRVDDEKMSKSLGN
                     FFTIREVLKEFDAEVVRFFILRAHYRSALNYSDSHLEDARSALTRLYTALKRLPDDEE
                     FTAAVDWGEAHAQRFRAAMNDDFNTPEAVAVLFDLANETNRQQSVPLARQLRALGQVI
                     GLLTRPANAFLQAASGDDSSLTPVRIDELIGERAAAKKARDFSRADCIRAELLAQGIV
                     LEDGPQGTTWRRA"
     gene            2189..>2504
                     /locus_tag="VIS73_06490"
     CDS             2189..>2504
                     /locus_tag="VIS73_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762837.1"
                     /translation="MLPYFSIRERQRHATQPASRQPTITLRKILFASLLGVCACLVQA
                     QETGKELAEANRLLKQGKHAAALSTVDGHLAQNPNDAQGRFLKGLILTEQNRLEDATA
                     VFV"
BASE COUNT          440 a          854 c          745 g          465 t
ORIGIN      
        1 cgcccgaacc tgcgtagtgc cctcccggat ttgtcgaacc cctgcccgcg ccgcgtccat
       61 cgcctgtgtt gccagcccct gcgccgtttc ccaacgctcg ccatcggctg cggtcgcagc
      121 gtcgaccagc aatcggtcga cggcggcgac ctgcgtcgcc gcgccctctc tcccggcttc
      181 ctggctcagt cgaagcaggg catcacgcag cgcgccagcg ctgtcacgaa cgcgttcgta
      241 gctttgccgg gtcggcgccc gaacgctggc ggccggcgcc tcgtttcgtg gcgcattctg
      301 ttcctgcgcc agcaggcgga tttcggcacg caccagcgca taagcctggc cgagcagcgc
      361 cggcgcctga tcccgactgc ccttcgcctc gccaatcagc cggtcgacct cggccaggac
      421 cgtccggcga tcctggccgc gctgcgcggc aacccgctct agcgcatcgc gcagaaccat
      481 taccgacgcc tccagtcctg cgcccggcgt cgattccgcc agcgccgctg aactgccgga
      541 cagcacaatc gccgcgctca ccagacacgc gccccagaac gaatcagatc cttgcatgtc
      601 ggcctccctg ccgcttgttt gatggccctg cgctaagcgc gtcgccaggt agtcccttgc
      661 ggaccgtctt caaggacaat tccctgcgcc agcagctcgg cgcgaatgca atcggcccgc
      721 gaaaagtcgc gcgccttctt ggcagcagcc cgctcgccga tcagttcgtc gatccgcacc
      781 ggcgtcagcg acgaatcgtc gccgctcgcg gcctgtagga aagcattcgc cggcctggtg
      841 agaagcccga tgacctgccc cagcgccctc aactgccggg ccagcggaac gctctgctgg
      901 cgattggtct cgttggccag atcgaagagc acggcgaccg cttcgggcgt attgaagtca
      961 tcgttcatcg ccgcccggaa acgctgggcg tgcgcctcgc cccaatcaac ggcggctgtg
     1021 aattcctcat cgtcgggcag acgcttcagc gccgtgtaca aacgcgtcag cgcactacgg
     1081 gcgtcttcga ggtggctgtc cgagtagttg agggcgctgc ggtaatgcgc gcgcaggatg
     1141 aagaagcgga cgacttcggc atcgaactcc ttcagcactt cgcggatcgt gaagaagttg
     1201 ccgagcgatt tcgacatctt ctcgtcgtcg acgcgaacga agccgttgtg catccagtag
     1261 ttgacgaagg tcttgccgtt agcagcctca gactgcgcaa tttcgttttc gtggtgcgga
     1321 aactgcagat ccatgccgcc gccgtgaatg tcgaagtgtt cgccaagcag gcaggaactc
     1381 atcgccgaac actcgatgtg ccagcccggc cgcccctcgc cccacggcga tgcccacttg
     1441 gcttcggccg gttcttcagc cttggcatgc ttccagagta cgaagtcaag cgggtcgcgc
     1501 ttggcgctgt cgacttcgac gcgctcaccc gcccgaagat cgtcgatcga cttgcccgag
     1561 agcctgccgt agggctcgaa gcagcgcacc gcgaagttca cgtcgccgtc atcggcgcga
     1621 taggcaaagc cgcgtgtctc gagttccgag atcatcgcca gcatctgtgg cacatagtcg
     1681 gtcgcccgcg gctcgtgatc ggggcgcagc acaccgagcg catcggcgtc ttcgtgcatg
     1741 agcccgatgt agcgttcggt cagcgcgcgg atcgactcgg cgttctcgcc ggcgcggcgg
     1801 atgatcttgt cgtcgatgtc ggtgatgttg cgaacgtaat cgaccgcgta gccgctcgcc
     1861 cgaagccaac ggtagaccat gtcgaatacc accatcacgc gcgcgtggcc gaggtggcag
     1921 aggtcgtaga ccgtcatccc gcagacgtac atgcgcacct gcccgggcgt gagcgggacg
     1981 aactcctgtt tctcacgcga cagcgtgttg tagattctga gcatttgttt tggtcgggat
     2041 ggaaggccag cggctagaat cggcagaatt cgacggacta atcagcaaca cccgcgagtc
     2101 gccttcgcgt gcttgggaat cgctgtagcc tgttgatata attctttgtt tgttaagcca
     2161 tttaggctaa gcgcccgtat attacgccat gctcccctac ttctcaatcc gcgagcggca
     2221 gcggcacgcc actcagcccg cgtcgcgcca gcccaccatc acgctgcgaa aaatcctatt
     2281 tgcatccctc cttggggtct gcgcatgtct ggttcaggcc caggaaaccg gcaaggaact
     2341 ggccgaggcc aaccggctgc tcaaacaggg aaagcacgcc gcagcgctaa gcaccgttga
     2401 cgggcatctg gcacagaatc cgaacgacgc ccaaggtcgc tttctcaagg gcctgatcct
     2461 gaccgagcag aaccgcctcg aagacgccac ggccgtattc gtca
//
LOCUS       DATDAA010000201        11827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_329727,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000201 DATDAA010000000
VERSION     DATDAA010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11827
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329727"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VIS73_06495"
     CDS             complement(<1..576)
                     /locus_tag="VIS73_06495"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5762838.1"
                     /translation="MQIGRYQVIRELGRGAQSVVYLARDPQLDRPIAIKTMHFATHAN
                     SASQRGNAALLGEARIVGKLRHPNIVPIFDTGEAEGDPYLVFEYVPGKTLAQVLRETG
                     AMPPLKTAQLMEQVLDAVEHAHSKGIVHRDLKPSNILLDESGAVRVMDFGIAAKIAAP
                     RDGEDEYYGTPFYMAPEYIVKRAIDVRSDVFS"
     gene            complement(612..1037)
                     /locus_tag="VIS73_06500"
     CDS             complement(612..1037)
                     /locus_tag="VIS73_06500"
                     /inference="COORDINATES: protein motif:HMM:NF023125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HEY5762839.1"
                     /translation="MPGRDRRRLEQAFGQTCYRGVVAGERLELRVGQACPALDSRLTE
                     GLGWAWLTAVNPGGESLPDAENAARLASLRAVLGGTRIECWPGEAVADDGSWPAEPGI
                     VLVNVSLAAALAIAADFGQAAILWSDGGNPARLEWPASP"
     gene            complement(1037..2392)
                     /gene="rmuC"
                     /locus_tag="VIS73_06505"
     CDS             complement(1037..2392)
                     /gene="rmuC"
                     /locus_tag="VIS73_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY5762840.1"
                     /translation="MNDNLMLALAAIALLLLAVTLVLAWRASVKSDAAAIQLTAIEAG
                     LERAMRELREELARGRSEAGEEARRDRGEQGEAMGRLAQSLGQQLVQLGGAQGERLES
                     FARQLGQVTQNLDQRFERLREVVDQRLQAMQTDNAKKLDDLRRTVDEKLHQTLEQRLG
                     ESFKIVSERLEQVQRGLGEMHALAQGVGDLKRVLTNVKTRGTWGEVQLGTMLEQVLTA
                     EQYAANVATRPGSKERVEFAIRLPGGGDGEPVWLPIDAKFPLEDYQRLIDAQERVDQV
                     AVEAAAKALEMRLRDEAKKIRAKYVEPPHTTDFAILYLPVEGLYAEALRRAGLSDVLQ
                     RDYRVTLAGPTTLAAMLNSLQMGFRTLAIEKRSSEVWTILGAVKHEFEQFGDALAHTK
                     KKLNEAANSISKAETRSRVLGKKLRDVEALPGETAEALLGSVDELDELDEADTDEAAP
                     S"
     gene            complement(2420..3166)
                     /gene="gloB"
                     /locus_tag="VIS73_06510"
     CDS             complement(2420..3166)
                     /gene="gloB"
                     /locus_tag="VIS73_06510"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEY5762841.1"
                     /translation="MIDIVPIPAFRDNYIWAICRGDEVVVVDPGDDAPVRKFLAGRRL
                     AAILVTHHHADHVGGVAVLGGRNPVFGPGAESITGVSEPLSGGEQITLPGVGVELRVL
                     AVPGHTRGHIAYYGEGALFCGDTLFGAGCGRLFEGTPEQMHRSLKAFDTLPDDTLVYC
                     AHEYTMQNLRFAVRVEPDNADIAARVARCNAARERGEPTVPSTLGEERRTNPFLRSEE
                     AAVIRAAQARGAGSEPVEVFAAIRAWRDEF"
     gene            3198..3935
                     /locus_tag="VIS73_06515"
     CDS             3198..3935
                     /locus_tag="VIS73_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5762842.1"
                     /translation="MSIPGRTHWLSTPLGRYVSEWEAQRADQMLADVFGFNAVQLGLP
                     QIDLLRANRITLRAHADETGIVDVRCDPQQLPFASASLDLVVLPHVLEFHGHPHQVLR
                     EVERVLIPEGRLLVLGFNPFSLWGLRRRLPGCPPDYPFNGRYLGVARLKDWLALLNMD
                     VERGHFGCYAPPVSRLKNIARFAWMEKAGDRWWPIGGGVYMIRAVKRVPGMRLMLPGW
                     KRGAVRRKSLAPVLPTQPSSHARRKND"
     gene            3913..4362
                     /gene="rnhA"
                     /locus_tag="VIS73_06520"
     CDS             3913..4362
                     /gene="rnhA"
                     /locus_tag="VIS73_06520"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEY5762843.1"
                     /translation="MPDAKTIEIYTDGACRGNPGPGGWGALLRQDGHERSLCGGEAQT
                     TNNRMELTAVIRALKALKRPVVARIHTDSQYVRNGILTWIHGWKRNGWKTSDRKPVKN
                     VDLWQELDQLAAQHQLEWIWVKGHAGDPGNEAADQLANRGIDELQAR"
     gene            4359..5078
                     /gene="dnaQ"
                     /locus_tag="VIS73_06525"
     CDS             4359..5078
                     /gene="dnaQ"
                     /locus_tag="VIS73_06525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEY5762844.1"
                     /translation="MKQIVLDTETTGLDPKLGHRLIEVAGLVVANRRVTPTHFHRYLN
                     PEREVDAGALAVHGLSDEFLADKPKFADVAADFLEFVRDAELIIHNAAFDVGFLNHEL
                     AMLNLPPVEKVCAQVTDTLKMARERHPGKRNNLDALCERYQVSNAHRKLHGALLDAEL
                     LAEVYLAMTRGQDSLIDDYDDPEHAGVSSRSGGAPRKPLIVLAASEAELQAHAQVLAN
                     IDRESKGNCLWLNDRDPVQSS"
     gene            5218..6876
                     /locus_tag="VIS73_06530"
     CDS             5218..6876
                     /locus_tag="VIS73_06530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5762845.1"
                     /translation="MKIRHKITLAFGAAVSVGLIALAAFYIQHQEDAVLAQNERTMAK
                     LTESVSQGLQAVMLSGYADMAQSFAERLKSVSEVREFRILRTNGDEAFHDNATIAAVN
                     RQHGRETFHPRSTEDRVPVLAPDDANLRRAIETMAPVASYATDPLGERTLTFLAPIKG
                     IDVCGACHDAHEPVRGIIQLTTSLEPVERELSAVRKTSLIVLVVALVFTIVLTGFVLG
                     RTVVLPLEKITAAMVRVAGGDLTHKVPLESKDEFGRMAKSFNQMTTELQGTYDGLRRE
                     QDKLTTIIHGVGEGIVVTNGAGEVVLVNPAAERLLGRDARAIAAAGFDALFDEPQVVR
                     GLLDGSIAAPHTLLHRERMLNVHATTINGDDGKPVGSTALLRDVTEEKRFEELLRLQS
                     ATDALTGLYNRRHLDETLSVEFHRAVRTGTCLSVLMLDIDHFKSFNDTHGHDQGDRVL
                     QGVADSLRRTLRKYDIACRYGGEEFVAILPDTDGILAERVGERLRHDVETCSIDGLSV
                     TISIGIASTPELVVDTPEALLEAADGALYASKKAGRNRTTVAAG"
     gene            7207..8322
                     /locus_tag="VIS73_06535"
     CDS             7207..8322
                     /locus_tag="VIS73_06535"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEY5762846.1"
                     /translation="MQPTVKPKNPNFSSGPCSKRPGYNVAALKLDTLGRSHRSALGKK
                     ALGLACTETARILGLPEGYRVAVVPASDTGSVEMAMWSMLGARGVDALAWESFGAGWI
                     TDIVKQLKLPNVTKHEAAYGEIVDLAKVNFDNDVVFTWNGTTSGVRVPDGKWIPADRA
                     GLTICDATSAVFAMELPWDKLDVVTFSWQKVLGGEGAHGMLIIGPRAVARLESYSPPW
                     PMPKIFRMTSGGKLTEGLFKGETINTPSMLCVADYLDALAWVDSLGGVKATIARSEAN
                     LKIVSDFVAANPWITFLAEDQAIRSNTSVCLTLKASEEQVKKLVKLLETEGVAYDIGA
                     YKDAPAGIRIWCGATVEARDLQALMPWLAWGYAQVTA"
     gene            8356..9534
                     /locus_tag="VIS73_06540"
     CDS             8356..9534
                     /locus_tag="VIS73_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HEY5762847.1"
                     /translation="MFRIRTYNQISIKGLERFPRQDYEVGSDIGHPDAFLLRSQKLHG
                     VEVPDSLLGVARAGSGFNNIPVAEYSKRGIVVFNTPGANANAVKELVMAGMLLATRGI
                     LPGMAYVQSLTTMTDSAEMAALLEKEKSRFAGGEIKGKTLGIVGLGAIGSMVADMALA
                     MGMKVIGFDPALSIDAAWRLSNAVGRMENLPSLLAHSDYVSLHVPALDATHHMINEAA
                     LASIKRGAVLLNFARETIVDPAAVRASLDSSRLGRYVCDFPEPGLLGRPDVIATPHIG
                     ASTEESEENCAVMAANQLIDFLQNGNIVNSVNFPAVSMPRTPGTARITFSNDNVAGVL
                     GHVLSVFADHRVNVVDMMNKSRADLAFNIIDVEGAPSAQVAAAIAATPSVIRVRVLAP
                     "
     gene            complement(9506..9931)
                     /locus_tag="VIS73_06545"
     CDS             complement(9506..9931)
                     /locus_tag="VIS73_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762848.1"
                     /translation="MSSPYLTVTAVIAGVTVLQGANGLLLVLLPLRMTAKGFSVQTMG
                     WVAAAHGAGFLIGCLATSRLIALIGHVRAFSALAATLSVVALALLPHTPTTRVIGTAT
                     RTPVGRATKPTRIWDLRSRRSTIAFRGRGRLRGRGREHE"
     gene            complement(9941..10405)
                     /locus_tag="VIS73_06550"
     CDS             complement(9941..10405)
                     /locus_tag="VIS73_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762849.1"
                     /translation="MRQFLLNVATVLVLAACSHGPVDPVAGHLEAGEAVHLRVAATLM
                     HGHDMQEPEWDSAQGYRLPPAYAGELLVTDRRLLFVNADPVELSIPYAAISRSRPSAT
                     PLLNYLIVWDHEGHPDSFVVDAADVKALHAGFARALYRARGRRMASPAGRPE"
     gene            complement(10544..11764)
                     /gene="icd"
                     /locus_tag="VIS73_06555"
     CDS             complement(10544..11764)
                     /gene="icd"
                     /locus_tag="VIS73_06555"
                     /inference="COORDINATES: protein motif:HMM:NF005425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY5762850.1"
                     /translation="MAEKITMQDDGTLSVPDNPIIPFIEGDGIGIDITPVMIKVIDAA
                     VAKAYGGKKQIHWMEVYAGEKSTRLYGPDQWLPAETFAALKEYSVSIKGPMTTPVGGG
                     IRSLNVALRQELDLYQCVRPVQYFKGVPSPLKHPELCNMVIFRENTEDIYAGIEWANG
                     SEQVKKVIKFLQEEMGVRKIRFPETSGIGIKPISVEGTERLVRAAIKYAIANGRKSVT
                     IVHKGNIMKFTEGNFRDTAYALAKREFGAVELDGGPWMKLPADKGGIVIKDSIADAFL
                     QQILLRPAEYDVIATTNLNGDYISDALAAQVGGIGIAPGANISDVYACFEATHGTAPK
                     YAGKDKVNPGSLILSAEMMLRHLGWTEAADLVIKSMEAAIGEKQVTYDFARLMEGATE
                     VSCSGFGDAMIARM"
BASE COUNT         2086 a         4037 c         3670 g         2034 t
ORIGIN      
        1 cgagaacacg tcggagcgca cgtcgatggc acgcttcacg atgtactcgg gggccatgta
       61 gaaaggcgtg ccgtagtact cgtcctcgcc atcgcgcggc gcggcaatct tcgccgcgat
      121 gccgaagtcc atgacacgca cggcacccga ttcgtcgagc agaatattcg atggcttgag
      181 atcgcgatgg acgatgccct tcgaatgcgc gtgctcgacg gcgtcaagca cctgctccat
      241 cagctgggcg gtcttcaggg gcggcatcgc gccggtttcg cgcaagacct gcgcgagcgt
      301 cttgccgggc acgtactcga agaccaaata cggatcgcct tcggcctcgc cggtatcgaa
      361 gatcggcacg atgttggggt ggcgcagctt gccgacgatg cgcgcctcgc cgagcagtgc
      421 cgcgttaccg cgctggctgg ccgaattggc gtgcgtcgcg aagtgcatgg tcttgatcgc
      481 aatcggccga tcgagctgcg gatcgcgcgc gagatagacc acgctctgcg cgccgcgccc
      541 aagttcccga atgacctgat accgaccgat ctgcacgcgc ctacccagtt ccgccctaac
      601 cggccgtcat tctaggggct tgccggccat tcgaggcgtg caggattgcc gccgtcgctc
      661 caaagaatcg cggcctggcc gaaatcagcc gcaatggcca gcgccgctgc gaggctaacg
      721 ttgacgagaa cgatgccggg ttcggccggc cagcttccgt cgtcggctac ggcctcgccg
      781 ggccagcatt cgattctagt gccccccaag accgcacgca gcgacgccag ccgcgccgcg
      841 ttctcggcat cgggcaggct ttccccgccg gggttcaccg ccgtcaacca agcccagccg
      901 aggccttcgg tcagtcgtga atcgagggct ggacaagcct ggccgactcg cagctcaagg
      961 cgctcgccgg cgaccacgcc gcgatagcac gtttgcccaa acgcctgctc gagcctccgg
     1021 cggtcgcggc cgggcatcaa ctcggcgcag cctcgtcggt gtccgcttcg tccagttcgt
     1081 ccagttcatc gacagaacca agcagcgcct cggccgtttc cccgggcaac gcctcgacgt
     1141 cgcgcagctt cttgccgagc acgcgcgaac gcgtctcggc cttgctgatc gaattggccg
     1201 cttcgttgag cttcttcttg gtgtgcgcca gcgcatcgcc gaactgctcg aactcgtgct
     1261 tgaccgcgcc gaggatggtc catacctcgg acgaacgctt ctcgatcgcc agcgtgcgaa
     1321 agcccatctg cagactgttg agcatcgccg ccagcgtcgt cggacctgcc agcgtgacgc
     1381 gatagtcgcg ctgcagtacg tcgctcaatc cggcgcggcg caaggcctca gcgtagagcc
     1441 cctcgaccgg caggtaaagg atcgcgaagt cggtggtgtg cggcggctcg acgtacttgg
     1501 cgcggatctt cttcgcctcg tcgcgcaggc gcatctccag cgccttcgcc gcggcctcga
     1561 cggcgacctg atcgacgcgc tcctgcgcgt cgatcaggcg ctggtagtcc tcgagcggga
     1621 acttggcgtc gatcggcagc cagaccggtt cgccgtcgcc gccgccgggc agccggatcg
     1681 cgaactcgac gcgctccttg ctgccgggcc gggtcgcgac gttggccgcg tactgctcgg
     1741 cggtcagcac ctgttcgagc atggtgccga gttgcacctc gccccaggtg ccgcgggttt
     1801 tgacgttggt cagcacgcgc ttgagatcgc cgacgccctg tgccagcgcg tgcatttcgc
     1861 cgagcccgcg ctgcacttgc tcgagccgct cgctgacgat cttgaaggat tcgccgaggc
     1921 gttgctcgag cgtctggtgc agcttctcgt cgaccgtacg acgcaggtcg tcgagcttct
     1981 tcgcgttgtc ggtctgcatc gcctgcaggc gctggtcgac cacctcgcgc aggcgttcga
     2041 agcgctggtc gagattctgc gtgacctgcc cgagctggcg agcgaaggat tcgaggcgct
     2101 cgccctgcgc accgccgagc tgcaccagtt gctgcccgag cgattgcgcc aagcgcccca
     2161 tcgcttcgcc ctgctcgccg cggtcgcggc gcgcttcctc gccggcctcg ctgcgtccgc
     2221 gcgccaactc ctcgcgcagc tcgcgcatcg cccgctcgag gccggcctcg attgcggtta
     2281 gctgaatcgc tgccgcgtcg ctcttcaccg atgcgcgcca ggcgaggacg agcgtgaccg
     2341 ccagcaatag cagggcgatg gcagcgagtg ccagcatcag gttgtcgttc atcggggttt
     2401 ccggaacgtg ggcgccgtct cagaattcat cgcgccaggc gcgaatcgcg gcgaagactt
     2461 ccactggttc gctcccggcg ccgcgcgcct gcgcggcgcg gatcaccgcg gcttcctcgc
     2521 tgcgcaggaa cggattggtc cggcgctctt cgccgagcgt cgacggcacc gtcggctcac
     2581 cgcgctcgcg cgccgcgttg cagcgtgcga cgcgcgcggc gatgtcggca ttgtcgggct
     2641 cgacgcggac ggcgaagcgc aggttctgca tcgtgtattc gtgcgcgcag tagaccagcg
     2701 tgtcgtcggg gagtgtgtca aaggccttga gcgaccgatg catctgctcc ggcgtaccct
     2761 cgaacagccg accgcatccg gcgccgaaca gcgtgtcgcc acagaaaagc gcgccttcgc
     2821 cgtagtaggc gatgtggccc cgcgtgtggc cgggcacggc gagtacgcgc agttcgacac
     2881 cgacgccggg cagcgtgatc tgctcgccgc cggagagagg ctcgctgacc cctgtgatag
     2941 actcggcgcc cggcccgaac acgggatttc gcccgccaag cacggcgacg ccgccgacgt
     3001 gatccgcgtg atgatgcgtc accaagatcg ccgccagtct ccggccagcc aggaatttcc
     3061 ggaccggcgc atcgtcaccg ggatcgacca cgacaacctc gtcaccgcgg caaatcgccc
     3121 agatgtagtt gtcgcgaaaa gccggaatcg gaacaatatc aatcattgtc ggcaacctta
     3181 ggcgagcgcc gctaccgatg tcaattcccg gacgtaccca ctggttgagc acgccgctcg
     3241 ggcgttacgt gagcgagtgg gaagcacagc gagccgacca gatgctcgcc gacgtcttcg
     3301 gcttcaacgc ggtgcagcta ggcctgccgc agatcgacct gctgcgcgcc aaccgcatca
     3361 cgctgcgcgc tcatgccgac gagaccggca tcgtcgatgt ccgctgcgat ccgcagcaac
     3421 ttcccttcgc cagcgccagc ctcgacctcg tcgtgctgcc gcacgtgctt gagttccacg
     3481 gacatccgca tcaggtcctg cgcgaagtcg agcgcgtgct gattcccgaa ggccggctac
     3541 tggtgctcgg tttcaacccg ttcagcctct ggggcctgcg ccgccgcctg ccgggttgcc
     3601 cgcctgacta tccattcaac ggccgctacc ttggcgtcgc ccggctcaag gactggctgg
     3661 cgctgctcaa catggacgtc gagcgcggtc atttcggctg ctacgcgccg ccggtatcgc
     3721 ggctgaaaaa tattgcgcgc ttcgcctgga tggagaaagc cggcgatcgc tggtggccga
     3781 tcggcggcgg cgtctatatg atccgcgcgg tcaagcgcgt tcccggcatg cggctgatgc
     3841 tgcccggctg gaaacgcggc gccgtgcgcc gtaagtcgct ggcccctgtc cttcccacgc
     3901 agccctcttc ccatgcccga cgcaaaaacg attgagatct ataccgacgg cgcctgccgc
     3961 ggcaatcccg gccccggcgg ctggggcgca ctgctgcgcc aggacggcca cgagcgatcg
     4021 ctgtgcggcg gcgaggcgca gacgaccaac aaccgcatgg agctgaccgc ggtaatccgc
     4081 gcactcaagg cactcaagcg ccctgtcgtc gcccgcatcc ataccgactc gcagtacgtt
     4141 cgcaatggta tcctcacttg gatccacggc tggaagcgga acggctggaa gacctcggac
     4201 cgcaagccgg tgaagaacgt cgacctctgg caggaactcg atcaactcgc cgcgcagcac
     4261 cagctcgaat ggatctgggt caagggccac gccggtgacc ccggcaacga agccgccgat
     4321 cagctcgcca atcgcggcat cgatgaactg caggcacgat gaaacagatc gttctcgaca
     4381 ccgaaaccac cggcctcgat cccaagctcg gccatcggct gatcgaagtc gcgggcctgg
     4441 tcgtcgccaa tcggcgtgtg acgccgacgc acttccaccg ctacctcaat cccgaacgcg
     4501 aagtcgacgc cggcgcgctg gccgtgcacg ggctgtcgga cgaattcctc gccgacaagc
     4561 cgaagttcgc cgatgtcgcc gccgactttc tcgagttcgt ccgcgatgcc gagttgatta
     4621 tccacaacgc cgccttcgac gtcggtttcc tcaaccacga actggcgatg cttaacctgc
     4681 cgcccgtgga aaaagtctgc gcgcaggtca ccgacacgct gaagatggct cgcgaacggc
     4741 atccaggcaa gcgcaacaac ctagatgcgc tgtgcgagcg ctatcaggtc agcaacgccc
     4801 accgcaagct gcacggcgcc ctgctcgatg ccgagctgct cgccgaggtc tacttggcga
     4861 tgacgcgcgg ccaggacagc ctgatcgacg actacgacga tcccgagcac gccggcgtca
     4921 gcagccgctc cggcggtgcg ccacgcaagc cgttgatcgt gctcgcagca agcgaagccg
     4981 agctgcaggc gcatgcgcag gtacttgcca acattgatcg cgagagcaag ggcaactgcc
     5041 tctggcttaa cgatcgcgat ccggtgcagt cgtcctgagg agcgccgcct tgccgatcga
     5101 tcggtggccg ctcgaccccg ctcgggtcaa tgacatgcac ttggcgggtt cttgcctgcc
     5161 cgccgggtca gcgtagaatc cgccaatgca aaagcgcctc gcccatcgcc gcccccgatg
     5221 aagattcgcc acaagatcac gctcgccttc ggtgccgccg tttcagttgg cctcatcgcc
     5281 ctcgcagcct tctacatcca gcaccaagaa gacgcggttc tcgcccagaa cgaacggacg
     5341 atggccaagc tcaccgagag cgtctcgcag ggtctgcagg cggtgatgct ctcgggctac
     5401 gccgacatgg cgcagtcctt cgccgagcgc ctgaagtcgg tctcggaagt ccgcgaattc
     5461 cgaatcctgc gcaccaacgg cgacgaagct ttccacgaca acgcgacgat cgccgcggtc
     5521 aatcgccagc acggcaggga aaccttccat ccgcgtagca ccgaagaccg cgtcccggtc
     5581 ctcgcccccg acgacgcgaa tctgcgccgc gccatcgaga cgatggcacc cgtggccagc
     5641 tacgccaccg accccttagg cgaacgcaca ctgaccttcc tcgcgccgat caaggggatc
     5701 gatgtctgcg gcgcctgcca cgatgcccac gagccggtgc gcggcatcat ccagctgacc
     5761 acctcgctcg aaccggtcga acgcgaactc tcggcagtgc ggaagacctc gctgatcgtg
     5821 ctggtcgtcg cactggtgtt caccatcgtg ctcaccggct tcgtcctcgg ccgcaccgtc
     5881 gtcctgccgc tcgagaaaat caccgcggcg atggttcgcg ttgccggcgg cgacctcacg
     5941 cacaaggtgc cgctcgaatc gaaggacgaa ttcgggcgca tggcgaaaag cttcaaccag
     6001 atgactaccg agttgcaggg cacctacgac ggcctgcggc gcgaacagga caagctgacg
     6061 acgatcatcc acggcgtcgg cgaaggcatc gtcgtcacca atggcgccgg cgaggtggta
     6121 ctcgtcaatc cggccgccga acgcctgctc ggccgcgacg cgcgcgcaat agccgccgcc
     6181 ggcttcgacg cactgtttga cgaaccgcag gtcgtccgcg gcctgctcga cggatcgatc
     6241 gccgcaccac acacgctgct ccaccgcgaa cgcatgctca acgtccacgc caccactatc
     6301 aacggcgacg acggcaagcc ggtcggctcc accgccctgc tgcgcgacgt caccgaagaa
     6361 aagcgtttcg aggaactgct gcgcctgcag tcggcgaccg acgcattgac cggcctctac
     6421 aaccgccgcc acctcgacga aacgctgtcg gtcgagttcc accgcgccgt gcgcaccggc
     6481 acctgcctct cggtgctgat gctcgacatc gaccacttca agtcattcaa cgacacccac
     6541 ggccacgacc agggcgaccg cgtgctgcaa ggagttgccg actcgctgcg caggaccctg
     6601 cgcaagtacg acatcgcctg ccgctacggc ggcgaggagt tcgtcgccat ccttccagac
     6661 accgacggaa tcctcgccga acgagtcggc gagcgccttc gtcacgatgt cgagacctgc
     6721 agtatcgacg gcctgtcggt caccatcagt atcggcattg cgagtacccc cgaactcgtc
     6781 gtcgacaccc ccgaggccct gctcgaagcc gccgacggcg cgctctacgc gtcaaagaag
     6841 gcgggtcgaa accgaacgac ggtcgcggcg ggctgaactc ccagtatcgg ccaggagcgg
     6901 ccggtcgagc gctccctgaa tccgtcattc agaggtctgg tcgtcgctaa aagctgccgg
     6961 acgacgaagg cgaactgata ggcaattacc gcctttgtac ttcccgacgg actcaggcgg
     7021 caacgcatcg atcacctgcc gttcaaatgg ctttctggtc gaacggcagt ttctcgcgcc
     7081 aactaatttg aggccgccga gcttcattgc ccgcgataga gtcctgattg attgccccga
     7141 ccgccccgga gcagcataat ttcgcactcc atccggcttt ccctgttcat ctgacgcgag
     7201 accacgatgc aaccgactgt caaaccgaag aatcccaact tctcctccgg tccgtgcagc
     7261 aagcgtccgg gctataacgt cgccgccctg aagctcgaca cgctcggccg gtcgcaccgg
     7321 tcggcgctgg gcaagaaggc gctcggcctc gcctgcaccg agactgctcg catcctgggc
     7381 ctgccggagg gttaccgggt ggccgtggtg ccagcctcgg ataccggttc cgtcgaaatg
     7441 gcgatgtggt cgatgctcgg cgcacgtggc gtcgatgcct tggcctggga atcgttcggc
     7501 gccggctgga tcaccgacat tgtcaagcag ctgaagctgc cgaacgtcac caaacacgag
     7561 gcggcgtacg gcgaaatcgt cgacctagcc aaggtcaact tcgacaacga cgtcgtcttc
     7621 acgtggaacg ggaccacctc gggcgtcagg gttcccgacg gcaaatggat ccctgccgac
     7681 cgcgccggcc taaccatctg cgatgccacc tcggccgtgt tcgccatgga actgccctgg
     7741 gacaagctcg acgtcgtcac cttttcgtgg cagaaggtgc tcggcggcga aggcgcccac
     7801 ggtatgctga tcatcggtcc gcgtgccgtc gcccggctgg aaagctactc gccgccgtgg
     7861 ccgatgccca agattttccg catgacctcg ggcggaaagc tcaccgaagg cctgttcaag
     7921 ggcgaaacca tcaacacgcc ttcgatgctc tgtgtcgccg actacctcga cgcgcttgcc
     7981 tgggtcgaca gccttggcgg cgtcaaggcc actatcgcgc gcagcgaggc caacctcaag
     8041 atcgtctccg atttcgtcgc ggctaacccg tggatcacgt ttctggccga ggatcaggcg
     8101 atccgctcta acaccagtgt ctgcctcacc ctcaaggcca gcgaggagca agtcaagaag
     8161 ctcgtcaaac tactcgaaac cgaaggagtc gcctacgata tcggcgccta taaggacgcc
     8221 cctgcgggca tccgcatctg gtgcggagct acggtcgagg cccgcgacct gcaggccttg
     8281 atgccctggc tggcttgggg ctacgctcag gtcaccgcct gagcagccgg gcatacagac
     8341 acggcgaggg ggagcatgtt caggatccgc acctacaacc agatctcgat caaggggctg
     8401 gagcgctttc cgcgccagga ctacgaagtg gggagcgaca tcggtcaccc cgatgccttc
     8461 ctgctgcgca gccagaaact gcacggcgtc gaggtacccg acagtctgct cggcgtggcg
     8521 cgcgccggtt ccggcttcaa caacattccg gtcgccgaat acagcaaacg cggcatcgtg
     8581 gtattcaaca cgccgggcgc caatgccaac gccgtcaaag aattggtcat ggccggcatg
     8641 ctactcgcca cgcgcggcat cctgcccggc atggcctacg tccagtcgct caccacgatg
     8701 accgactcgg ccgaaatggc cgccctgcta gaaaaagaga aatcccgttt cgcgggaggt
     8761 gaaatcaagg gcaagacgct gggcatcgtc ggcctcggtg ccatcggctc gatggtcgcc
     8821 gacatggctc tggcaatggg catgaaagtg atcggctttg atcccgcgct ctcgatcgat
     8881 gctgcttggc ggctgtcaaa cgccgtcggc cgcatggaaa acctaccgtc gctgctggcc
     8941 cactccgatt acgtctcgct gcatgtgccg gcgctggatg caacccacca catgatcaac
     9001 gaagcggcgc tggcctcgat caagcggggc gccgttcttc tcaatttcgc gcgcgaaacc
     9061 atcgtcgatc cggctgccgt ccgcgccagc ctcgacagca gccgactcgg ccgctacgtc
     9121 tgtgatttcc ccgagcccgg actgctcggc cggcccgacg tgatcgcaac cccccacatc
     9181 ggcgccagca ccgaagagtc cgaagaaaac tgcgcggtca tggcggccaa tcagctgatt
     9241 gactttctgc agaacggcaa catcgtcaat tcggtcaatt tcccggcggt cagcatgccg
     9301 cgcacgcccg gtacggcgcg catcacattc tcgaacgaca atgttgccgg cgtgctcggc
     9361 cacgtcctgt cggtcttcgc cgaccatcgg gtcaacgtag tggacatgat gaacaagagc
     9421 cgcgcagatc tcgccttcaa catcatcgac gtcgaaggcg cgcctagcgc ccaggtggca
     9481 gcggccattg ccgcgacccc tagcgtcatt cgtgttcgcg tcctcgcccc ctgagtcggc
     9541 cgcgccctcg gaacgcgatc gtagacctcc gcgatcgtag atcccatatc cgggtaggtt
     9601 tcgtagcacg accaacaggc gttcttgttg cggtcccgat aacgcgcgta gtaggcgtgt
     9661 gcggcagcaa ggccagcgcc acgaccgaca gggtcgctgc cagcgccgag aaggcgcgca
     9721 cgtgaccgat cagggcaatc agcctgctgg tggcgaggca accgatcagg aatccggcgc
     9781 cgtgggcggc ggcgacccac cccatcgtct gcaccgagaa gcccttggcc gtcatgcgca
     9841 gcggcagcag gacgagcagc aggccgttgg ctccctgcag cactgtcacg ccggcgatga
     9901 cggcggtgac agtgaggtag gggctggaca tcgccttcgg ctactccggc cgtccggcgg
     9961 gtgacgccat ccgtcggcca cgcgcgcgat acagcgcccg ggcgaagccg gcgtgcaagg
    10021 ctttgacgtc cgcagcatcg actacgaagc tgtcgggatg tccttcgtga tcccagacga
    10081 tcaggtagtt taggagcggc gtcgcactcg gccgcgagcg cgagatcgcc gcgtagggga
    10141 tgctaagctc gaccgggtcg gcgttgacga acagcaggcg ccggtcggtg accagcagtt
    10201 caccggcata cgccggcggc aatcggtaac cttgggccga atcccactcg ggctcctgca
    10261 tgtcatgccc gtgcattagc gtcgcggcga cacgcagatg cacagcttcg cccgcttcga
    10321 ggtgcccggc caccgggtcg accggcccgt gcgaacaggc ggccagtacc agtacggtgg
    10381 cgacgttcag caggaattgg cgcatcgcat cagggaaaat cgaaatcgga gccgacacgt
    10441 tacaccagtg gctttccatc ccatgcggtg cggcgtcgcc acaccaacga aaaggccccg
    10501 cgtcgcgggg cctcatagcg agggtctttc gtcggatgcg caactacatc cgcgcgatca
    10561 tcgcgtcgcc gaaacccgaa caggagactt cggtagcgcc ctccatcagg cgggcgaagt
    10621 cgtaggtgac ctgcttttcg ccgatcgccg cttccatgga cttgatgaca aggtcggctg
    10681 cctcggtcca gcccaggtgg cgcagcatca tctcggcgga gaggatcagc gagccgggat
    10741 tgaccttgtc cttgccggcg tacttgggtg cagtgccgtg ggtcgcctcg aagcaggcgt
    10801 agacgtcgga gatgttggcg cccggcgcga tgccgatgcc gccgacctgc gcggcgagcg
    10861 cgtcggaaat atagtcgccg ttgaggttgg tggtggcgat cacgtcgtac tcggccgggc
    10921 gcagcaggat ctgctgcagg aaggcgtcgg caatcgagtc cttgatgacg atgccaccct
    10981 tgtcggcggg cagtttcatc cacggtccgc catcgagttc gacggcgccg aactcgcgct
    11041 tggccagcgc gtaggcggtg tcacggaagt tgccttcggt gaacttcata atgttgccct
    11101 tgtggacgat ggtcaccgac ttgcgcccgt tggcgatcgc gtacttgatc gcagcccgta
    11161 ccagtcgctc ggtgccttct accgagatcg gcttgatgcc gatgcccgag gtctcgggga
    11221 agcgaatctt cctgacgccc atttcctcct gcaggaactt aatgaccttc ttcacctgtt
    11281 cggagccgtt ggcccactcg atgccggcgt atatgtcctc ggtgttctcg cggaagatca
    11341 ccatgttgca cagttcgggg tgcttgagcg gcgaaggcac gcccttaaag tactgaaccg
    11401 ggcgcacgca ctgatagagg tcgagttcct gtcgcagcgc gacgttgagc gagcggatgc
    11461 caccgcccac cggcgtggtc atcgggccct tgatcgagac tgagtattcc ttaagcgcgg
    11521 cgaaggtctc ggcgggcagc cactggtcgg ggccgtagag acgagtgctc ttctcgccgg
    11581 cgtagacctc catccagtgg atctgcttct tgccgccgta ggccttggcg accgcggcat
    11641 cgatcacctt gatcatcacc ggcgtgatgt cgatgccgat gccgtcgccc tcgatgaagg
    11701 gaatgatcgg gttgtcggga acggagaggg tcccgtcgtc ctgcatcgtg atcttctcag
    11761 ccatgcggct gaccttagcg ggtcgactca ggacgcctgc gcccgggaga gcgcgacgcg
    11821 ctcgcag
//
LOCUS       DATDAA010000202         3247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_120491,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000202 DATDAA010000000
VERSION     DATDAA010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120491"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /gene="tsaD"
                     /locus_tag="VIS73_06560"
     CDS             complement(<1..690)
                     /gene="tsaD"
                     /locus_tag="VIS73_06560"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041224676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY5762851.1"
                     /translation="MIVLGLESSCDESGVALYDTDRGLLAEALHSQVAMHEAYGGVVP
                     ELASRDHVRRLLPLLDRVLAEAGVPPAAIDAVAYTQGPGLAGALLVGAAVAESLALAL
                     QKPVIPIHHLEGHLLSPLLARPAPQFPFVALLVSGGHTQLMEVDAVGEYRLLGESLDD
                     AAGEAFDKSAKLLGLGYPGGPALAALAVSGRPGALKLPRPMIASGDLNFSVSGLKTAV
                     LTAVRAHGERVP"
     gene            849..1061
                     /gene="rpsU"
                     /locus_tag="VIS73_06565"
     CDS             849..1061
                     /gene="rpsU"
                     /locus_tag="VIS73_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY5762852.1"
                     /translation="MPNIRVKENEPFEVAIRRFKRTVEKTGLLTELRAREFYEKPTAE
                     RKRKLAAAVKRQHKRLRSQILPPKLF"
     gene            1172..1627
                     /locus_tag="VIS73_06570"
     CDS             1172..1627
                     /locus_tag="VIS73_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY5762853.1"
                     /translation="MSLKARITDDMKAAMKARETERLGAIRLLLAAIKQREVDERVEL
                     DETAVLAIVEKLIKQRRDSITQYEAAQRKDLAAKERFEADLLATYLPARLTAAEISTE
                     IERAASECGAASAADMGKLMALLKPRLAGRADMGEVSRLVKARLSGTPS"
     gene            1668..>3247
                     /gene="dnaG"
                     /locus_tag="VIS73_06575"
     CDS             1668..>3247
                     /gene="dnaG"
                     /locus_tag="VIS73_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY5762854.1"
                     /translation="MIPEGFIQDLLARVDIVDLIDGHVPLKKAGANFAACCPFHNEKT
                     PSFTVSPSKQFYHCFGCGAHGTAIGFLMEYSGLGFVESVKDLAQRTGMQVPDDGGRPE
                     PTRRGPDLLGVMSAASRYYRDQLKHSQRAIEYLKSRGLSGETAARFGIGYAPDGWQNL
                     EAAFPDYANESLQTAGLVIRNEGGRTYDRFRDRIMFPILSAKGEVIAFGGRVIGDGEP
                     KYLNSPETPLFEKGRELFGLPQARQGLREQNLAIVVEGYMDVVALSQHGVGNALATLG
                     TATTPEHARKLFRQVDQVVFCFDGDAAGRKAAWRALENTLDIIVDNKQIAFAFLPEAD
                     DPDSFVRQHGADAFRGFVRRALPLSEYLVQELARRHPPDSAEGRVALLHDAKPLLGKI
                     ASPAYRLQLVKRLGDITETTPGEIESLCGLRPVAALPAAKRVVRATPSLARSVARLIL
                     RQPALAAQLPDEALPGGTEGGALRALRDAAISGQTPTTAALIERLHGVADPRWLEALS
                     AEVISHPLDDDACQTEFA"
BASE COUNT          587 a         1120 c         1042 g          498 t
ORIGIN      
        1 cggaacccgc tcgccgtgcg cccgcacggc cgtcagcacg gctgtcttca gaccactgac
       61 actgaaattg agatcgccac tggcaatcat tggtcgcggc agcttgaggg cgccgggtcg
      121 ccccgacacg gcgagcgcgg ccagcgccgg cccgcccggg taaccaagcc cgaggagctt
      181 ggcgctcttg tcgaaagcct cgcccgccgc atcgtcgagc gactcgccga gcaggcggta
      241 ttcgccaacc gcgtcgactt ccatcaactg cgtatgcccg cccgagacca gcagggcgac
      301 gaaggggaat tgcggcgccg ggcgcgccag gagcggcgac agcagatggc cctccaggtg
      361 atggatgggg atgaccggct tctgcagcgc cagcgccagt gattcggcga ccgcagcgcc
      421 gaccagcagc gcgccggcga gaccgggccc ttgcgtatag gccacggcgt cgattgccgc
      481 tggcggcacg ccagcctccg cgagcacccg gtcgagtagc ggcagcaaac ggcggacatg
      541 gtcgcgcgag gccagttccg gcaccacacc accgtaggcc tcgtgcatcg ccacctggga
      601 atgcagcgcc tcggcgagga ggccccgatc ggtgtcgtac agcgcgacgc cgctttcgtc
      661 gcaggagctc tcgagaccaa ggactatcat cgtggcggcg attctatccg gtcgcactac
      721 cgcgacgcgc cacctcgaac ccatcggtgt gcgcttcggc tcgacaaacg aagccgacct
      781 gcgctagact ctgcgccctt cgctaaacca accacgcttc aaccgaggca atttggggtc
      841 tttgttcgat gcctaacatc cgtgtcaagg aaaacgagcc gttcgaagtg gccatccgcc
      901 gcttcaaacg caccgtcgaa aaaaccggtc tgctgaccga actgcgcgcc cgcgagtttt
      961 acgagaagcc caccgccgag cgcaagcgca agctggcagc cgcggtcaag cggcagcaca
     1021 agcgtctgcg cagccagatt ctgccgccca agctcttcta agcagctgac caatttgttg
     1081 cagcggccgc cctcttcgtt gagggcggct tttgtcattt gtacgaccga gactgcgccg
     1141 ctgttccggc gaacccgtag gaggactcac gatgtcgctc aaggcccgga ttaccgatga
     1201 catgaaggct gcaatgaagg cgcgggaaac cgaacgcctg ggcgcgattc ggctgctgct
     1261 ggcggcaatc aagcagcgcg aggtcgacga acgggtcgaa ctcgacgaaa cggcggtcct
     1321 ggcgatcgtc gaaaagctca tcaaacagcg gcgcgacagc atcacccagt acgaagcggc
     1381 ccagcgtaag gatctggccg ccaaggaacg gtttgaggcg gacctgctgg cgacctacct
     1441 gccagcccgg ctcaccgccg ccgaaatctc caccgaaatc gagcgcgcag ccagcgagtg
     1501 cggcgctgcg tcggcggccg acatgggcaa gctgatggcg ctgctcaagc ccaggctggc
     1561 ggggcgcgcc gacatgggcg aggtatctcg cctggtcaag gcacggttgt ccggcacacc
     1621 gtcctgatcc ggcaccagct ggaatcgagc gcgacggcgc gccggccatg attcccgaag
     1681 gcttcatcca ggacctgctg gcccgggtcg acatcgtcga cctgatcgac gggcacgtgc
     1741 cgctgaagaa ggccggcgcc aatttcgccg cctgctgccc cttccataac gagaagaccc
     1801 cctcgttcac ggtcagcccg tcgaagcagt tctatcactg ctttggttgc ggcgcgcacg
     1861 gcaccgcgat tggcttcctg atggaataca gcggcctcgg tttcgtcgag tcagtaaaag
     1921 atctcgccca gcgtaccggc atgcaggtcc ccgatgacgg aggccgcccg gaaccgacgc
     1981 ggcgcggccc cgatctgctc ggggtgatgt cggccgcgtc gcgctattac cgcgatcagc
     2041 tcaagcactc gcagcgcgcc atcgaatacc tgaaaagccg cgggctcagc ggcgagaccg
     2101 cggctcgctt cggtatcggc tacgcgcccg atggctggca gaacctcgaa gctgccttcc
     2161 cagactatgc gaacgagtcg ctgcagaccg ccggcctcgt catccgcaat gaaggcggcc
     2221 gcacctacga ccgatttcgc gaccggatca tgttccccat cctcagcgcc aagggcgagg
     2281 tcattgcttt tggcggacgg gtgatcggcg atggcgaacc gaagtacctc aattcaccgg
     2341 aaacaccgct gttcgagaag ggccgcgagc tgttcggtct gccgcaggcg cgtcagggat
     2401 tgcgcgaaca gaacctggca atcgtcgtcg aaggctacat ggatgtcgtc gccctatcac
     2461 aacacggcgt gggcaatgcg ctggcaacgc ttggcactgc cacgacgcca gagcacgcgc
     2521 gcaagctgtt ccgccaggtt gaccaggtgg tcttctgctt cgacggtgac gccgcggggc
     2581 gcaaggcggc gtggcgcgca cttgaaaaca cgctggacat catcgtcgac aacaagcaga
     2641 tcgccttcgc cttcctgcct gaggccgacg acccggacag cttcgtgcgg caacatggcg
     2701 ccgacgcgtt ccgcggcttc gtccgccgcg cgctgccgct gtcggagtat ctggtgcagg
     2761 agctggcgcg ccgccacccg cccgacagcg ccgaggggcg cgtcgccttg ctccacgacg
     2821 cgaagccgct gctgggcaag atcgcctcgc cggcgtatcg gctgcaactg gtcaaacgcc
     2881 tgggcgacat caccgaaacg actcccggcg aaatcgagtc gctgtgcggg ctgcgccccg
     2941 ttgccgcgct gcccgccgcc aagcgcgtgg tccgggcgac cccctcgttg gcgcggagtg
     3001 tcgcccgcct gattctccgc cagccggcgc ttgccgcgca gcttcccgac gaggcgctgc
     3061 ccggcgggac cgaaggcggc gcgctgcgcg ccctgcgtga cgcggccatt tccggccaga
     3121 cgccgaccac cgcagcactg atcgagcgcc tgcacggggt tgccgacccg cgctggctgg
     3181 aggccctgag cgccgaagtc atcagccatc cgcttgacga cgatgcctgc cagacggagt
     3241 ttgccga
//
LOCUS       DATDAA010000203         2127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_295535,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000203 DATDAA010000000
VERSION     DATDAA010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295535"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="VIS73_06580"
     CDS             <1..1121
                     /locus_tag="VIS73_06580"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HEY5762855.1"
                     /translation="LDELETRIAQIESTSDRQADQLAQAAANDKSSDWASRLKWKGDL
                     RYRHEMIDEETRDQRNRQRIRARVGLEAKVSDNLKTYVGIATGDPEDPRSTNATLGGG
                     NVRKNIALDYAYFDWAAFDNTTVSLGKMKYPFERLAGSQLFYDGDVNPEGGSIRYKAD
                     NGLFASGYGFWITENSSSADPHVFGAQLGWESTFGLTLAAAYNDYTVKDMQLGISLDS
                     GAPLAGNSFYGCGAGGAAACYLYDYNILGLDAEYGFKLRSMPVKVWASYIENTAINDL
                     NTGYNIGFMFGKAKDQGSWEIGALYQDLEKDAQWGGVSDSDFAGGITQGKGFVVKGAY
                     VPVKNTSLNLAYFDNTKNYGTASARDYQRLQLDFSMKF"
     gene            1266..2000
                     /gene="phoU"
                     /locus_tag="VIS73_06585"
     CDS             1266..2000
                     /gene="phoU"
                     /locus_tag="VIS73_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY5762856.1"
                     /translation="MEPADLGHHISRRFNEDLEKVRTRVLQMGGFVEQQLQQAVTALV
                     ESDSALGEEVANADHQVNQMEVTIDEECGRIIAMRQPTASDLRVIVAIIKTITDLERI
                     GDEIEKIAMIASRLAATEQPSDKYRDVRHLARLVIDMVHDSLHAFARLDSAEAMEVAR
                     RDRAVDVEYEAIQRQNITYMMEDPRSIRRALDIMWIVRALERIGDHAKNMCEYIVYTV
                     HGKDIRHLSIEDAEAQILASKADRNA"
BASE COUNT          439 a          678 c          679 g          331 t
ORIGIN      
        1 agctcgatga gctggagacc cgcatcgccc agatcgagtc caccagcgat cgccaggccg
       61 accagctcgc gcaggccgcg gcgaacgaca agagcagcga ctgggcgagc aggctgaagt
      121 ggaagggcga ccttcgctat cgtcacgaga tgatcgacga ggagaccagg gaccagcgca
      181 atcgccagcg gatccgcgcg cgggtcggtc tggaggcaaa ggtgtccgac aacctcaaga
      241 cctacgtcgg catcgccacc ggcgacccgg aggatccgcg ttcgacgaac gcgaccctcg
      301 gcggcggcaa cgtccgcaag aacatcgccc tcgactacgc gtacttcgac tgggcggcct
      361 tcgacaacac gacggtctcg ctcggcaaga tgaagtaccc gttcgagcgc ctcgccggca
      421 gccagctgtt ctacgacggc gacgtcaacc cggagggcgg ttcgatccgc tacaaggccg
      481 acaatggcct gttcgcgagc ggctacggtt tctggatcac cgagaactcc agttccgccg
      541 atccgcatgt cttcggtgcg cagcttgggt gggagtcgac cttcggcctg accctggcgg
      601 ccgcctacaa cgactacacg gtgaaggaca tgcagctggg catttcgctg gacagcgggg
      661 cgccgctggc cggcaactcc ttctacggct gtggtgccgg cggcgccgct gcctgctacc
      721 tgtacgacta caacatcctt ggcctggatg ccgagtacgg cttcaagctc cgatcgatgc
      781 cggtcaaggt gtgggccagc tacatagaga ataccgcgat caatgacctg aacaccggct
      841 acaacatagg tttcatgttc ggcaaggcca aggaccaggg cagctgggaa atcggcgctc
      901 tctaccagga tctcgagaaa gatgcccagt ggggcggcgt gagcgactcc gacttcgccg
      961 gcggcatcac gcagggcaag ggtttcgtcg tgaagggcgc ctacgttccc gtcaagaaca
     1021 cgtcgctgaa cctggcgtac ttcgacaaca cgaagaacta cgggacggcc agcgcgcgag
     1081 actaccagcg cctgcagctc gacttcagca tgaagttctg atgtgaagag gtgcctggca
     1141 cgtacctggc ctctccccgg tgccaggtac gtgccaggca ccgggtcaag gcgccaggtc
     1201 ggcgcggcct gaccccgcgt tttcggttac tatttcgcca gcaatccagg acgccaggca
     1261 ctgccatgga acccgcagat ctcggtcacc acatttcccg ccgcttcaac gaggacctcg
     1321 agaaggtccg cacgcgcgtc ctgcagatgg gcggcttcgt cgagcagcaa ttgcagcagg
     1381 cggtcacggc cctggtcgag agcgacagcg ccctcggtga ggaggtcgcc aatgccgatc
     1441 accaggtcaa ccagatggaa gtgaccatcg acgaggagtg cggccgcatc atcgccatgc
     1501 gccagccgac ggcgtcggac ctgcgcgtca tcgtcgccat catcaagacc atcaccgacc
     1561 tcgagcggat cggcgacgag atcgagaaga tcgcgatgat cgcctcgcgg ctggccgcca
     1621 ccgagcagcc ctccgacaag tatcgcgacg tgcggcacct ggcgcggctg gtcatcgaca
     1681 tggtgcacga ctcgctgcat gccttcgcca ggctcgattc cgccgaggcc atggaagtgg
     1741 cgcgtcgcga ccgcgcggtt gacgtcgagt atgaggcgat ccagcgccag aacatcacct
     1801 acatgatgga agacccgcgc agcatccgtc gcgcgctcga catcatgtgg atcgtgcgtg
     1861 cgctcgagcg catcggcgac cacgccaaga acatgtgcga gtacatcgtc tacacggtgc
     1921 acggcaagga catccgccac ctgtcgatcg aggatgccga ggcgcagatc ctggcctcga
     1981 aagcggatcg gaatgcgtga agcaagagag cggatagcgg atagcggata gcggatagcg
     2041 gatggccaga ggggggcagg aacagccgaa ccccgttcac ttgattgggt cggagacaaa
     2101 ccaggcgctg cgccttaagt tcgcacg
//
LOCUS       DATDAA010000204         3609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_259788,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000204 DATDAA010000000
VERSION     DATDAA010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259788"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VIS73_06590"
     CDS             <1..342
                     /locus_tag="VIS73_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5762857.1"
                     /translation="VLHDVERIADRFGFINKGEIVTTRSPQSLVGESAGRMLVRFQCS
                     AEAGLGGQVLRDGEFELELEQAALPEWLAQLNAKGGRLLTVKPEMSLERVFFQILEKG
                     AQPKPEPAATQ"
     gene            complement(422..964)
                     /locus_tag="VIS73_06595"
     CDS             complement(422..964)
                     /locus_tag="VIS73_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5762858.1"
                     /translation="MSRDRSSSSNIEAVASAATTDHEAKTTMDILLWRHAEAEDGAED
                     MKRKLTARGEKQARCVAVWIREHSRKNLRVLVSPSVRTQQTATALDLPYETDRHLAPG
                     ARPTDLLAACGWPDGDDSRSSVLVVGHQPTLGELAALLMTGHVAPWSVKKGALWWLTR
                     KSRGEQAQIVLRTVIDPALL"
     gene            complement(961..2460)
                     /locus_tag="VIS73_06600"
     CDS             complement(961..2460)
                     /locus_tag="VIS73_06600"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEY5762859.1"
                     /translation="MPREIELKLRLDPAAVEIMARHPALSGVRPVAKRLCNTYYDTAD
                     LSLRRSGIALRFRRSGRQWMLTVKGGNPGAGGLAARDEWEVPRRPGDFDFGHVDDEAI
                     RQTLEAARSTLAPLFTTDFRRVIWRIPSPAGGGIEIALDVGTITAGRRSEALCEVELE
                     RHEAEDSAPLFALAGELAETLPLHPEARSKAERGYALFGKLAEKPRKTAPTPVNVRMS
                     PIEAFQAIAFDCLDQLLRNETGAIAGDDPEFVHQARIGIRRLRSALGLFAPILPASLV
                     QDYAGPWREVANALGGGRDWDVLMHETLPRVARFNARHGSDYSPPKALTRAAARAHEK
                     ALATTRVALLHPGYSRFLLQFSAAAHGLGDADAPIVPGALAPFARARMDRQLRRVWRI
                     VATTHLKTHLHRHELRIELKKLRYATDFFAPLIPAKPLARYRKRVAALQDILGLLNDI
                     TVGRELISSLKHRKGPDPLDNWLATDHARVIKGLAGALKRLRRANPPWQ"
     gene            complement(2562..3266)
                     /locus_tag="VIS73_06605"
     CDS             complement(2562..3266)
                     /locus_tag="VIS73_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY5762860.1"
                     /translation="MTQRPPTINIPALLARVPLFHGLAIDEVNRIAAGAREVDVARGK
                     ILFQRGDASDGFYIVVYGQMKLAFTGASGTEKVVEIISQGQSFGEAVMFMDKPYPVMA
                     QALADSQLVHIGKAALFQELDRDPQLGRKIIAGLSMRLHRLIADVESYSLRSGRERVI
                     GYLLRNQDDTDTHRDVLIELPTKKGVIASRLNLTQEHFSRILHDLAESGLIVVDGRRI
                     RIPDVARLSGAGEPSA"
     gene            complement(3291..>3609)
                     /gene="panD"
                     /locus_tag="VIS73_06610"
     CDS             complement(3291..>3609)
                     /gene="panD"
                     /locus_tag="VIS73_06610"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEY5762861.1"
                     /translation="EGSCAIDQDLLDAADIQEYEQIDIWNINNGERFTTYAISAERGS
                     GIISINGSAARKAAPGAPLIIATFASYDEKEAGAHEPALVYLDSQNRIVNTKRKIPVQ
                     LAG"
BASE COUNT          565 a         1084 c         1294 g          666 t
ORIGIN      
        1 gtgctgcacg acgtcgagcg gattgccgat cgcttcggtt tcatcaacaa gggagagatc
       61 gtcactactc gttcgccgca gagtctggtc ggcgaatccg cggggcggat gctggtccgt
      121 ttccagtgtt cagccgaagc cggtctgggc ggtcaggttc tccgcgacgg ggagttcgaa
      181 ctggaactcg agcaggccgc gcttcccgag tggctggcgc agctcaacgc gaaaggcggg
      241 cggttgctga ccgtaaagcc ggagatgtct ctcgagcggg tgttcttcca gattctcgaa
      301 aagggcgcgc agcccaagcc ggaaccggcc gcgactcagt aattcgctcg gcgactggtc
      361 gcattgcttc gttgcgcggc tggattgcag ccgacgcgac gaccgctacg acgaatcgga
      421 tctacagcag cgccggatcg atcaccgtgc gcaggacgat ctgcgcttgt tctccgcgcg
      481 atttgcgcgt cagccaccac agggcgcctt tcttgaccga ccacggggcg acgtgccccg
      541 tcatcagcag ggcggcgagt tcgcccagcg ttggctggtg gccgacgacg agcacgctgc
      601 tgcggctgtc gtcgccgtca ggccagccgc aggcggcgag caggtcggtc ggccgggcgc
      661 cgggcgccag gtggcggtcg gtttcatagg ggaggtccag tgcggtcgcg gtctgttggg
      721 tgcggacgga ggggctgacc aggacgcgta gattctttcg gctgtgctcg cgaatccaga
      781 cggcgacgca gcgggcctgt ttttccccgc gcgcagtgag tttgcgtttc atgtcctcgg
      841 cgccgtcttc ggcttcggcg tgccgccaga gcaggatgtc catggtggtt ttggcctcgt
      901 ggtcggtggt cgcggcggat gctaccgcct cgatgttgct ggatgacgag cgatcgcggc
      961 tcattgccac ggtggattcg ctcggcgcag gcgtttgagg gcgccggcca gccccttaat
     1021 gacgcgggcg tggtcggtgg ccagccagtt gtcgagcggg tcggggccct tgcggtgctt
     1081 gagcgaggag atcagctcgc ggccaacggt gatgtcgttg agcaggccga ggatgtcctg
     1141 cagcgcggcg actcgcttgc ggtaccgggc gaggggcttg gccgggatga gtggcgcgaa
     1201 gaagtcggtc gcgtagcgca acttcttcag ttcgatacgc aactcatgcc ggtgcaggtg
     1261 agtcttcagg tgcgtggtgg cgacgattcg ccagacacgg cgcaactggc ggtccatacg
     1321 cgcgcgcgcg aacggggcaa gcgcgccggg cacgatgggc gcgtcggcgt cgccaagacc
     1381 gtgcgcggct gccgagaact gcagcaggaa gcggctgtag cccggatgca gcagcgccac
     1441 ccgcgtggtc gcgagggcct tttcatgggc ccgggcggcg gcgcgggtga gggccttggg
     1501 cggtgagtaa tcgctgccgt gtcgggcgtt gaagcgggca acacgcggca gcgtttcgtg
     1561 catcagcacg tcccagtcgc ggccaccacc cagcgcgttg gcgacttcgc gccaggggcc
     1621 ggcgtagtcc tggaccagac tcgccggcag aatcggcgcg aagagcccga gggcagagcg
     1681 caggcggcgg atgccgatcc gcgcctgatg gacgaattcc gggtcgtcac cggcgatggc
     1741 gccggtttca ttgcgcagca attggtcgag gcaatcgaag gcgattgcct ggaaggcctc
     1801 gatcggcgac atgcgcacgt tgaccggtgt aggcgccgtc ttgcgtggtt tttcggcaag
     1861 cttgccgaac agcgcgtagc cgcgctcggc cttgcttcgc gcctctgggt ggagcggtag
     1921 cgtttcggcg agttcgccag ccagcgcgaa cagcggcgcg ctgtcctcgg cctcgtggcg
     1981 ttcgagttcg acctcgcaca gcgcctcgct gcggcgcccg gcggtgatcg tgccgacgtc
     2041 gagggcgatt tcgatgccac cgccggctgg ggaaggaatc cgccagatga cgcggcgaaa
     2101 gtcggtggtg aacagggggg ccagtgtgct ccgggctgct tctagcgtct gccggattgc
     2161 ctcgtcatcg acatggccga aatcgaagtc gccgggccgg cgcggcactt cccattcatc
     2221 gcgcgcagcg agaccgccgg caccaggatt gccgcctttt acggtgagca tccattggcg
     2281 gccgctgcgg cgaaagcgca gggcgattcc gctgcgccgc agcgagaggt ccgcggtgtc
     2341 gtaataggtg ttgcagaggc gcttggctac cggcctgacg ccggaaaggg cagggtgccg
     2401 cgccatgatc tcgacggcag cagggtccag gcgcagcttg agctcgatct ctcgcggcat
     2461 gtccgatccg gcatggaagt cccggcggag gcgccggtag catttgttct tgtgccgcgc
     2521 acgctagcac gccgcgatcg caggcgtcta tcgccttcgt attacgcgct gggctcgccg
     2581 gcgccgctca ggcgggcgac atcggggatg cgaatccgtc ggccatcgac cacaatcagc
     2641 ccgctttctg cgaggtcgtg aaggattcgc gagaagtgct cttgggtcag gttgaggcgt
     2701 gaggcaatca cgcccttctt ggtcggcagt tcgatcagga cgtcgcggtg agtgtcggta
     2761 tcgtcctgat tacgcaacag atagccgatc acgcgctcgc gtcccgagcg cagcgaatac
     2821 gactcgacat cggctatcag acgatgcagg cgcatcgaca gaccggcgat gatcttgcgg
     2881 ccgagttgcg gatcgcggtc gagttcctga aacagcgccg ccttgccgat gtggacgagc
     2941 tggctgtcgg cgagcgcctg cgccatgacc gggtagggct tgtccatgaa catcacagcc
     3001 tcgccgaagc tctggccctg gctgatgatc tcgacaacct tctcggtacc cgaggcgccg
     3061 gtaaaggcga gtttcatctg cccgtagacg acgatgtaga aaccgtcgct ggcgtcgcct
     3121 cgctggaaca ggatcttgcc gcgcgcgaca tcgacctcgc gagcgccggc ggcgatgcgg
     3181 ttgacctcat cgatggccaa cccatgaaac agcggaaccc tcgccaggag cgcagggatg
     3241 ttgatggtcg gtggtcgctg cgtcaccgga gggcggtccg gcggtggtcg ttatccggcg
     3301 agctgaacgg ggattttgcg cttggtgttg actatgcggt tctgcgagtc gagatagaca
     3361 agcgccggtt cgtgcgcccc ggcttccttc tcgtcgtagc tcgcgaaggt ggcgatgatc
     3421 aacggagccc ccggggcggc cttacgtgcc gcagaaccgt tgatcgaaat gatgccagag
     3481 ccgcgctcgg cggaaatcgc ataggtcgtg aagcgctcgc cattgttgat gttccagatg
     3541 tcgatctgct cgtattcctg gatgtcggcc gcgtcgagca ggtcctgatc aatcgcgcag
     3601 gaaccttcg
//
LOCUS       DATDAA010000205         2598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_434772,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000205 DATDAA010000000
VERSION     DATDAA010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434772"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(339..>2598)
                     /gene="clpB"
                     /locus_tag="VIS73_06615"
     CDS             complement(339..>2598)
                     /gene="clpB"
                     /locus_tag="VIS73_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY5762862.1"
                     /translation="ALADKGRVGELLKKAGATKNLVEKAIEEMRGGQQVNDPNAEDQR
                     QALEKFTIDVTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIINGEVPEGLKGKRLLSLDMGALIAGAKFRGEFEERLKGVLNDLSKQEGQVI
                     LFIDELHTMVGAGKAEGSMDAGNMLKPALARGELHCVGATTLDEYRQYVEKDAALERR
                     FQKVLVDEPSVEDTIAILRGLKERYEVHHGVDITDPAIVAAGVLSHRYISDRQLPDKA
                     IDLIDEAASRIRMEIDSKPEAMDKLERRLIQLKIERVALQKESDEASKKRLDIIEEEI
                     QSLEREFSDLDEVWKAEKAAVQGASHIKEELERARLELETSLRASDHARASELQYGRI
                     PELEKKLAAADAAESHDMQLLRNKVTEEEIAEVVSKWTGIPVSKMLEGERDKLLRMEE
                     AISQRVIGQSEAVKVVSDAIRRSRAGLSDPNRPNGSFLFLGPTGVGKTELTKALAAFL
                     FDTEEAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVE
                     KAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTVIVMTSNLGSEMIQELAGEDNYEVMK
                     SSVMTVVAGHFRPEFINRIDDVVVFHPLGRAQIRTIADLQINILRKRLEDREIGLQLT
                     EAALDLIGEAGFDPVYGARPLKRAIQTQLENPLSQQILSGEFGPGDVVSIEVREGALV
                     FSHGEQGAVSAA"
BASE COUNT          465 a          839 c          750 g          544 t
ORIGIN      
        1 gacctacgca ttcgcgtacc tgaaacgggt attgcccgga cgctggctcg atcacctgct
       61 ggcaaaagcg ggcggctgat tcagccgggg tttgaatgta tgcggggacg ctgaacgtca
      121 ccactgcgca aaggtaatcg caggagcccc ttgcaggcgc ggtaaccacg gaatgacccg
      181 gaaattccgg ttaaaacgaa accctgaaaa cagctgcagg agcgcaggaa tgcgcgaaca
      241 ttaatagccg atttattcgc ggacggcgtc cgctcctaca gcatataccc accgcacagg
      301 agcgccttgc aggcgcgaat ggggtgacat gggttacctc aggcggctga caccgccccc
      361 tgttcgccat gcgagaacac cagcgcaccc tcgcgcacct cgatgctgac cacgtcaccc
      421 gggccgaact cgccggagag aatttgctgg gatagcgggt tctccagctg cgtctggatc
      481 gcgcgcttca gggggcgtgc gccgtagacc gggtcgaagc ccgcctcgcc aatcaggtcc
      541 agcgcggctt cggtcagctg cagaccgatc tcgcgatctt ccaggcgctt gcgcaggata
      601 ttgatctgca ggtcggcaat ggtacggatc tgcgcccggc ccagcgggtg gaacaccacg
      661 acatcgtcga tacggttgat gaactcgggc cggaagtgcc cggcgaccac ggtcatcacc
      721 gaggacttca tgacttcata attatcctcg ccggccagct cctggatcat ttcgctgccg
      781 aggtttgacg tcatgacgat cacggtgttg cggaaatcca ccgtacggcc ctgcccgtcg
      841 gtaagacggc cgtcatcaag tacctgcaac agcacgttga acacatccgg gtgcgccttc
      901 tcgacctcgt cgagcaggat gaccgaatag ggtttacggc gaacggcctc ggtcaggtaa
      961 ccgccttcct cgtagcccac gtaaccgggc ggcgcgccga tcaggcgggc cacggaatgc
     1021 ttctccatga actcggacat atcgatgcgc accatggcct cttcggtatc gaacaggaag
     1081 gcggccagcg ccttggtcag ttcggtcttg ccgacgccgg tcggccccag gaacaggaac
     1141 gaaccgttcg gccggttcgg atcggacagg ccggcacgtg aacggcggat ggcatcggac
     1201 accaccttca cggcctcgct ttgcccgatc acgcgctggc tgatggcctc ttccatgcgc
     1261 agcaacttgt cgcgttcgcc ctcgagcatt ttcgataccg ggatgccggt ccacttcgag
     1321 acgacctcgg cgatctcttc ctcggtgacc ttgttacgta acagctgcat gtcatgcgac
     1381 tcggccgcat cggcggccgc gagtttcttt tccagttccg ggatacggcc atactgcaac
     1441 tcggatgcgc gggcatggtc gctggcacgc agtgacgttt ccagttccag gcgcgcccgc
     1501 tccagctctt ccttgatatg gctggcaccc tgcaccgccg ccttctcggc tttccagacc
     1561 tcgtcaagat ccgagaactc gcgctcaagt gactgaatct cctcttcaat gatgtcgagg
     1621 cgtttcttcg atgcctcgtc ggattctttc tgtaacgcca cgcgctcgat cttcagctgg
     1681 atcaggcggc gctcgagttt atccatcgcc tcgggcttgg aatcgatttc catgcggata
     1741 cgactggcgg cctcgtcgat gagatcaatg gccttgtccg gcaattgccg gtccgagatg
     1801 tagcgatgcg acagcacgcc ggccgccacg atggccgggt cggtgatatc cacaccgtgg
     1861 tgtacctcgt agcgttcttt cagtccgcgc aggatggcga tggtgtcttc cacgctcggt
     1921 tcgtcgacca gcaccttctg gaatcggcgt tccagtgccg cgtccttttc cacgtactgg
     1981 cggtactcgt ccagcgtggt ggcgccgaca cagtgcaact cgccacgcgc caatgcgggt
     2041 ttaagcatgt tgcccgcatc catggaacct tcggccttgc cggcgccgac catggtatgc
     2101 agctcgtcga taaacaggat cacctggcct tcctgcttgg acaggtcatt gagcacgccc
     2161 ttgagacgtt cctcgaattc accacggaac ttggcgcctg cgatcagcgc acccatatcc
     2221 agcgataaca gtcgcttgcc cttcaaacct tccgggactt ctccattgat aatgcgttgc
     2281 gccaggcctt cgacgatggc ggtcttgcca acgcccggct cgccgatcag taccgggttg
     2341 ttcttggtac ggcgctgcag cacctggatg gtgcggcgga tttcgtcatc gcggccgatg
     2401 accggatcaa gcttgccctg ctcggcacgc tcggtcacat cgatggtaaa tttctccagc
     2461 gcctggcgct ggtcctcggc attcgggtca ttgacctgct gcccgccgcg catctcttca
     2521 atcgccttct cgaccaggtt cttcgtggcg cccgcctttt tcagcaattc gccgacccgg
     2581 cccttgtcgg caagcgcc
//
LOCUS       DATDAA010000206         2455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_190259,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000206 DATDAA010000000
VERSION     DATDAA010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190259"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VIS73_06620"
     CDS             <1..201
                     /locus_tag="VIS73_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762863.1"
                     /translation="SAWLLTFRFNGGLQSILFINHETGDVVRRNPAGHIRRRAPKDCT
                     IHFKLLNISHRMRLCSPWTKRG"
     gene            252..2294
                     /locus_tag="VIS73_06625"
     CDS             252..2294
                     /locus_tag="VIS73_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY5762864.1"
                     /translation="MSGFAQVQLGARRRAGGRLARCALLALVATAAGRASADLDLLDL
                     ETLGNLTVSAASRYAQPLRDAPSAVQVIDREAIRRHGWRTLGEALQSLAGVYATSDRT
                     YTYLGTRAFLIGGDYSTRFLLLVDGARINDNIYEQALFSEEFPLDLALVERIEYAPGP
                     GSALYGSNALFGVVNVITSKNHELPPVSVAATATSNGWREGRATLVTSTDAGPSLLLS
                     VSGGRKSGRDQQYDDPTGNLVVAGGGPSPDGVARGLDSQEQEQFFARIEGDGFSLTGI
                     YSRRQVEPSTALYGTLFNDPGLFLEDGYASLSARYSRAMRDDLALETRVEYAEMRYDA
                     EYPFDDGAGNRYVNRDHVVGRWWATDLRARYWGLDDHTLLAGIDWQGDAKARQQAADI
                     GVTINPPVDTGRLRHRIGFYLQDAWSFAPGWQATLGLRHDRFSETGTVNSPRGALIWQ
                     PNSDSVLKLMAGRAYRLPNAYERDYGDGTTYLPSPDLQPERIRTLEAVWEQNLGGGQD
                     FALSLFDYQLDRLISQQDVAGTLQYQNTEAVSANGVEARWSRRWNDGARLAASASVHD
                     HDRQADPAGDMSPTWTAKLNGSHPLPGGSLLLAVEVSAIGEMDYLWQGSPQHLGSLAV
                     VDLVLTRARVVPGLDGHLRVRNLFDRDYGQPASSEVPVPVVPGDERRLEASIGYTF"
BASE COUNT          423 a          796 c          784 g          452 t
ORIGIN      
        1 tcagcctggc tgctgacctt cagattcaac ggcggccttc agtcaatcct gtttatcaac
       61 cacgaaaccg gtgatgtggt ccgaagaaac ccggcaggtc acattcgtcg ccgggcgccg
      121 aaggattgca caatccattt caagctgtta aacatttcgc atcgaatgcg tttgtgctca
      181 ccgtggacta agcgcggctg atataaaaat attcagttga atgccaacca ctggcatagg
      241 gaaaggggaa aatgtctgga ttcgctcagg ttcaacttgg cgcgcgacgc cgcgccggcg
      301 gtcgcttggc gcgctgcgcg ctgctggcgc tagtggcaac ggccgcgggc agagcgagcg
      361 ccgaccttga tctacttgat cttgagacgc tcggcaacct gacggtcagt gccgcgtcgc
      421 gctacgcgca gccgctccgc gatgcgccat cggcggtgca agtcatcgac cgcgaagcca
      481 tccgccgcca tggatggcga acgctcggcg aggcactgca aagccttgcc ggtgtttacg
      541 cgaccagcga tcgcacctac acctatctgg gcacgcgggc tttcctgatc ggcggcgact
      601 acagcacacg ttttctgctg ttggtagacg gtgcccgcat caacgacaac atctacgaac
      661 aggcgctgtt ctcggaggag tttccgctcg acctcgcgct ggtcgagcgc attgaatatg
      721 caccgggccc gggctcggcg ctgtacggca gcaacgcgct gttcggtgtc gtcaacgtca
      781 tcaccagcaa aaatcacgaa ctgccaccgg tatccgtcgc tgccactgca accagcaacg
      841 gttggcgaga agggcgggcg acgctggtga cgtccaccga tgccggtccc tccctcctgc
      901 tgtcggtgag cggcgggcgc aagagcggcc gcgaccagca gtacgacgac ccaaccggca
      961 acctcgtcgt cgccggcggc gggccctcac ccgacggcgt cgcgcgcggc ctcgacagcc
     1021 aggagcagga acagtttttt gcgcggatcg aaggcgatgg cttttcgttg accgggatct
     1081 actcgcggcg ccaggtggag ccgtctacgg cgctgtacgg caccctgttc aacgatccgg
     1141 ggctgtttct ggaagatggc tatgccagtc tgtccgcgcg ctattcgcgg gcgatgcgcg
     1201 atgatctggc gctggaaact cgcgtcgagt acgccgagat gcgctacgac gccgagtacc
     1261 cgttcgacga cggtgccggc aaccgctatg tcaatcgcga ccatgtggtc ggtcgctggt
     1321 gggcgactga cctgcgtgcg cgttactggg gtctcgatga ccacacgctg cttgccggaa
     1381 tcgactggca gggcgatgcc aaggcgcggc agcaggccgc cgacatcggt gtgaccatca
     1441 atccgccggt cgacaccggc aggctccgtc atcgtatcgg cttctacctg caggatgcgt
     1501 ggtccttcgc gccgggttgg caggcgacgc tcggattgcg ccacgaccgt ttctcggaaa
     1561 ccggcaccgt caacagtccg cgcggcgcgc tgatctggca gccgaattcc gactccgtgc
     1621 tcaagctgat ggcaggccgt gcctatcgcc tgcccaacgc ctacgagcgc gattacggcg
     1681 acggtacgac ctacctcccc agtcctgacc tgcagcccga acgaatccgc acgctggagg
     1741 cggtctggga gcagaatctc ggcggtgggc aggatttcgc gcttagccta ttcgactatc
     1801 agctcgaccg ccttatttcg cagcaggacg tcgccggcac gctgcagtac cagaataccg
     1861 aggcggtgag cgccaacggc gtcgaggcgc gctggtcgcg ccgctggaac gacggtgcgc
     1921 gcctcgctgc cagcgcctcg gtacacgacc acgatcgcca ggccgatccg gccggcgaca
     1981 tgagtccgac ctggacggcc aagcttaacg gtagccatcc cttgcccggc ggcagcttac
     2041 tgctggccgt cgaagtcagc gccattggcg agatggacta tctttggcag ggcagtccgc
     2101 agcatcttgg ttcgctggcc gtggtcgatc tggtgctgac gcgggcacgc gtcgtgccgg
     2161 gtctcgatgg ccacctgcgg gttcgcaacc tattcgaccg tgactacggg cagccggcct
     2221 cgtccgaagt gccggtgcct gtggttcccg gcgacgagcg gagattagag gccagcattg
     2281 gctacacctt ctgacagccg atgcgcgttg ccgcagggtt tcagtcacga tcggaatgcg
     2341 cggcgccgag gccggggcgt cgtcctcgcc gggctctccg tgcttgccgc cttgactttg
     2401 acgcccctcc gcgcggccga ggatctggcg ccgctcaagg tggccttcct ctaca
//
LOCUS       DATDAA010000207         6458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_365253,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000207 DATDAA010000000
VERSION     DATDAA010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365253"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..999
                     /locus_tag="VIS73_06630"
     CDS             157..999
                     /locus_tag="VIS73_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5762865.1"
                     /translation="MNAAPTPPSNVAAPRHDGPVVRAAAYLDTIGGRTLQATRPTGDA
                     IYLLANALSGIGDLFSPAGHYLRRTIVQQLYFTAVQAFWLVNLIGIALGILVILPLIG
                     FGVTDVETQARVIELALFHQLVPILAALVVIGRSGTAVTAEIGDLNQGGAIDSLLAMG
                     VEPHRFIVLPRVIGLTASLVLLGFWGNIAAIVGAGAFNAMQGHAGFGNLAAACAATLT
                     PVDIVFTALMLLCFGVFIALAHCHFGLRSASSVEVQRNLPKAFVASLVGVLAITVLFV
                     SVRP"
     gene            996..1757
                     /locus_tag="VIS73_06635"
     CDS             996..1757
                     /locus_tag="VIS73_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5762866.1"
                     /translation="MSVDPGTLITARGLVLKAFPEATPFDFAIAPGEHRLIVSEPEAG
                     KTSLTKTLLGIISPRAGTVWLAGDELDSLAPARLLEARRQFGVVYAADGLIPAWTGFD
                     NLALPLRLAGMADGHEIGARLDAWVERYRIPARWLGLSCGQLQRDARLTLALSRALIG
                     KPALLLLDGVPIDLAVGYSPRHGLSMLRDFIDDGGALLVLVRDAFADRVPEQAIGASF
                     RHARLVAGRLQSDDIDEPRRRPANANPTQPTNPGN"
     gene            1761..2720
                     /locus_tag="VIS73_06640"
     CDS             1761..2720
                     /locus_tag="VIS73_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY5762867.1"
                     /translation="MAQQRDRDRFYKRSVGVLVIFSVLAVLVMLRLYQQTAGPFRDTL
                     RLSATLPRADGVTVDTPVTLAGLKIGRVASVGLADDNRVRLELIIESRVADRIRSDSS
                     AVLTRPLIGVPFVDIVIGSADKPRLEAGGEIPAIRAPDLNDLVATLPQRLVAIDAVLD
                     NAQSLSADLRRMTKSLTANDGPIDQSFRQLARATDEAAKAAAQLNETLVDVRRVVAET
                     GHAVEATNAALNDVRGLAADLRPLGPKAGEIAGSIERSLANVEAVTAELRSIGPQLGP
                     AVAAGQSAIEEADEVLRAAKGSFLLRGNLPPPAGVPAAPVPRP"
     gene            2723..3547
                     /locus_tag="VIS73_06645"
     CDS             2723..3547
                     /locus_tag="VIS73_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762868.1"
                     /translation="MRVLTVFALATALLSACASAPPQPAAIAQYERDMNRAQDRFHEG
                     RLLAAAAAYRQAVAAGERYDDAARLAPALLGLAAVALELEEFAEAGSHYREAVREADR
                     AGLPGLRLNAEVGAAETVRRSGDCRAASVAGQALLARGPEPGVAARIQLLLANCLTAG
                     GDPRAALATLYAPADTQSPGLQAARLASEAAAHLALGDALAARQAAEAALAIDKHDRH
                     PPAIAADHRLLAEIAAGAGDPDSALAHALRAWRIFRHTGQSRAASRLLAAFPAITK"
     gene            3868..4209
                     /locus_tag="VIS73_06650"
     CDS             3868..4209
                     /locus_tag="VIS73_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762869.1"
                     /translation="MRHAPKLLPWLARRAGVSNELAHRLWRRAIQEAAAIAGSDHSAE
                     YHAVAIDRLLNLLEVESNGAANFETSTSAWRWRHPQRMARCAAVAAISITRIWSQLAT
                     DLSRRRAIPAR"
     gene            4285..>6458
                     /gene="leuS"
                     /locus_tag="VIS73_06655"
     CDS             4285..>6458
                     /gene="leuS"
                     /locus_tag="VIS73_06655"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041224677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY5762870.1"
                     /translation="MQEKFQPADIERAAQSHWAATQASRALEDADKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLARFHRMQGYNVLQPMGWDAFGLPAENAAIANGVPPAKWTW
                     DNIAYMKQQLQSLGFMIDWDRELATCQPSYYKWNQWLFLRMLEKGIAYPKTQVVNWDP
                     VDQTVLANEQVIDGRGWRTGALVEKREIPGYYLAITQYAEELLSALDGLPGWPERVRT
                     MQANWIGKSTGVRFAFPYEVDGHPEKLWVFTTRADTIMGVTFCAVAAEHPLATRAARD
                     NPELAAFIEECKRGSTMEADVATMEKKGMATGFSVRHPLTGASVPVWVGNYVLMGYGE
                     GAVMAVPAHDERDFHFAKQYGLPIKPVIAVGDQAFSEESWQEWYADKVGGICINSGKY
                     DGLGYEAASDAIAGDLAVRDLGNKQVQWRLRDWGVSRQRYWGCPIPLIHCAQCGIVPV
                     PDEQLPVVLPEDCVPDGSGNPLNKREDFLHCACPKCGGEARRETDTMDTFVDSSWYYA
                     RYCAPDNAEAMVDARTNYWMPVDQYIGGIEHAILHLLYSRFWTKVMRDLGLVTYDEPF
                     ANLLTQGMVLNHIFSRRNAKGGIEYFAPDEIDVITDEAGRITGATLKADGSAIDYNGI
                     GTMSKSKRNGVDPQSLIDQYGADTARLFMMFASPPEQTLEWSDAGVEGAHRFLKRLWK
                     VAHTHVGGGIVAACSTSSGLDEGQKDLRRKLHQTIAKVADDYG"
BASE COUNT         1076 a         2284 c         2073 g         1025 t
ORIGIN      
        1 agatcggaag tcatcggcgt acggcgcgta ggtgggcagc gacggactcg tgccgccgga
       61 caggctggcg attatacgag gcggcccatg gcgccccgct gttgcgccgc cacgccggct
      121 cgccaaagca gtgggcaaac gccgataatc gtcgccatga acgccgcgcc gacgccgccc
      181 tccaacgttg ccgcgccccg gcacgatggc ccggtggtgc gtgctgccgc ttacctcgac
      241 acgatcggcg gccggacgct gcaagcgacg cgacccaccg gcgacgcaat ttaccttctc
      301 gccaacgcgc tgtcgggaat tggcgacctg ttttcaccgg ccggacacta tctgcggcga
      361 acgatcgtgc agcagcttta cttcaccgcc gtccaagcct tctggctggt caatctgatc
      421 ggcatcgcgc tcggcatcct ggtgatcctg ccgctgatcg gcttcggcgt caccgacgtc
      481 gagacgcagg cgcgcgtcat tgagctggcg ctgttccacc agctggtgcc gattctcgcg
      541 gcgttggtcg tcatcggccg ctcgggaacc gctgtcaccg ccgagatcgg cgacctcaat
      601 cagggcggcg cgatcgacag cctgctggcg atgggcgtcg agccgcaccg tttcatcgtt
      661 ttgccgcggg ttatcgggct gaccgcctcg ctcgtgctgc tgggattctg gggcaacatc
      721 gcggcgatcg tcggtgccgg cgccttcaac gcgatgcagg ggcatgccgg cttcggcaac
      781 ctggcagcag cctgcgcggc gacgctgact ccggtcgaca tcgtgttcac ggcgctgatg
      841 ctgctctgct tcggcgtctt catcgcgctc gcccactgtc atttcggact gcgctcggcc
      901 tccagcgtcg aggtccagcg caacctgccg aaagccttcg tcgcctccct ggtcggggta
      961 ctggcgatca ccgtcctttt cgtctcggtt cgcccgtgag cgtcgatccc ggcacgctga
     1021 tcaccgcgcg cggcctggtg ctgaaggcct tccccgaagc gacgccgttt gatttcgcca
     1081 ttgccccggg cgagcaccgg ctgatcgtca gcgaacccga agcgggcaag acctcgctga
     1141 cgaagacgct gctcggcatc atttcgccgc gtgcgggaac ggtctggctg gccggagacg
     1201 aactcgattc gctggcgccg gcgcggctgc tcgaggctcg ccgccaattc ggcgtggtct
     1261 acgccgccga cggcctgatc cccgcctgga ccggtttcga caatctcgcg ctgccgctgc
     1321 ggcttgccgg catggccgac ggccatgaaa tcggcgcgcg tctcgacgct tgggtcgagc
     1381 gttaccgtat accagcgcgc tggctcggtc tgagctgcgg ccaactgcaa cgcgacgcac
     1441 ggctgacgtt ggcgttgtca cgggccctga tcggcaagcc ggcgctgctt ctgctcgacg
     1501 gcgtaccgat cgacctggcg gtcggctact cgccgcgtca cgggctgtcg atgctgcgcg
     1561 atttcatcga cgatggtggg gccttactgg tactcgtccg agatgcattt gccgaccgcg
     1621 ttcccgagca ggctatcgga gccagtttcc gccacgcccg gcttgtcgcc ggcagactac
     1681 aatcggacga catcgacgag ccccggcggc ggccggcaaa cgccaacccg acccaaccca
     1741 cgaacccagg caactgagcc atggcgcaac aacgcgatcg cgaccgtttc tacaagcgca
     1801 gtgtcggcgt gctggtgatc ttctcggttc tggccgttct cgtcatgctg cgcctctacc
     1861 agcagaccgc cggtcctttc cgcgacacgc tgcggctttc cgcgacgctg ccgcgcgccg
     1921 atggcgtgac cgtcgatacg ccggtgaccc tggccggcct caagatcggc cgtgtcgcca
     1981 gcgtcggcct cgccgatgac aaccgcgtcc ggctcgaact gatcatcgag tcgcgcgtcg
     2041 ccgaccgcat ccgcagcgat tcgagcgccg tgctcacgcg gccgctgatc ggcgtgccct
     2101 tcgtcgacat cgtcatcggc tccgccgaca aaccccgcct cgaggccggc ggcgagatcc
     2161 cggccatccg cgcgcccgac ctcaatgatt tggtcgcaac gctgccgcag cgtctggtgg
     2221 caatcgacgc ggtcctcgac aacgcgcagt cgctttccgc cgacctgcgt cgtatgacga
     2281 agtcgctgac ggcaaatgac ggcccgatcg atcaaagctt ccggcagctc gcccgcgcga
     2341 ccgacgaggc tgcgaaagca gccgcgcaac ttaatgagac gctggtcgac gttcgccgag
     2401 tcgtcgccga aaccgggcac gcggtcgaag cgacgaacgc cgcactcaat gacgtgcgcg
     2461 ggctggcggc cgacctgcgc ccgctcggcc ccaaggccgg cgagatcgcc ggctcgatcg
     2521 agcgctcgct ggccaatgtc gaagccgtca cggccgaact gcggtcaatc gggccgcagc
     2581 tcgggccggc tgtcgcggcc ggacagagcg cgatcgagga agccgacgag gtgctgcgcg
     2641 cggccaaggg cagtttcctg ctgcgcggca acctgccgcc gcctgccggc gtgcccgccg
     2701 ccccggtacc gcggccgtga ccatgcgtgt cctcaccgtg ttcgcgctgg caaccgcgct
     2761 gttgagcgcc tgcgcttcgg cgccgccaca gccggcagcg atcgcccagt acgagcgcga
     2821 catgaaccgt gcgcaggatc gtttccacga aggccgcctg ctggccgcgg cagcagccta
     2881 ccggcaggcc gtcgccgcgg gcgagcgcta cgacgacgcg gcacggctcg cgccggcgct
     2941 cctggggctc gcagccgtcg cgctcgaact cgaggagttt gccgaggctg gttcgcacta
     3001 ccgcgaggcc gttcgcgagg cggatcgcgc cgggctcccg ggacttcgcc tgaatgccga
     3061 agtcggcgcc gcggaaaccg tccgccgcag cggcgactgc cgggcggcct cggttgccgg
     3121 ccaagccttg ctggcacgcg ggccggaacc aggggtcgct gcgcgcatcc agctcttgct
     3181 ggcgaactgc cttaccgccg gcggcgatcc gcgcgcggcg ctggcgacgc tctacgcgcc
     3241 ggccgatacg cagtcaccgg gcctgcaggc agcccgattg gccagcgagg ccgcagcgca
     3301 tctggcgctc ggcgatgcgc tcgcggcgcg ccaggctgcc gaggccgcac tggcgatcga
     3361 caagcacgac cgccatccgc cggcgatcgc cgccgaccac cggctgcttg cggagatcgc
     3421 cgccggcgcc ggcgatccgg attccgcgct cgcacacgcg ctgcgcgcct ggcggatctt
     3481 ccgccatacg ggccaaagca gggcagcgtc gcggctgctc gcagccttcc cggcgatcac
     3541 gaagtaagca gacactccac agttagatct tccgggggcg cacgcgaaag acccgctgga
     3601 tcagtcgcct gcgggccagc aaagattggt cggaccaaaa atccaaggca tcagatcaaa
     3661 gctcggcacc actatgttgc agcgcagttt caaacgccgt tccgatgttt tcggtaaata
     3721 cttattgccc aaggggagcg cccaacctat actttgtgca tcgcagcatt tgcaattgtt
     3781 tcggtcacgg cccacgccac ccgcgaggcc gcgaagaccg aaccagtgcg acggctaccc
     3841 gccgcgctgc cgaaaggagc agtacccatg cgccacgccc caaaactcct cccctggctc
     3901 gctcgccgcg ccggcgtcag taacgaattg gctcaccgcc tctggcgtcg cgcgattcag
     3961 gaagccgcag ccatcgccgg cagcgaccac agcgccgaat accacgcggt cgccatcgac
     4021 cgcctgctca acctgctcga agtcgaatcg aacggcgctg ccaatttcga gacatccacc
     4081 agcgcctggc gttggcgcca cccgcagcgc atggcgcgct gcgccgccgt cgccgcgatc
     4141 agcatcacgc ggatctggtc gcaactggcg accgacctgt cacgccgccg agccatcccg
     4201 gcgcgctgaa agcgccgctc cggcgcggct ttgcggcaaa ccgggcgggt ataatcccgc
     4261 cctttctctt tgccgaaccc cgccatgcag gaaaaattcc agcccgccga catcgagcgt
     4321 gccgcccagt cgcactgggc cgccacccag gcctcgcgcg cccttgaaga cgccgacaag
     4381 ccgaagtact actgcctgtc gatgtttccc tacccgtcgg ggaagctgca catgggccat
     4441 gtgcgcaact acacgatcgg cgacgtgctg gcgcgctttc accggatgca gggctacaac
     4501 gtactgcagc cgatgggctg ggacgccttc ggcctgcctg ccgagaacgc ggcgatcgcc
     4561 aacggcgtgc cgccggcgaa gtggacttgg gacaacatcg cctacatgaa gcagcagctc
     4621 cagtcgctcg gcttcatgat cgactgggac cgcgagctgg cgacctgcca gccgtcctac
     4681 tacaagtgga accagtggct gttcctgcgc atgctggaaa agggcatcgc ctatccgaag
     4741 acgcaggtgg tgaactggga cccggtcgac caaaccgtgc tcgccaacga gcaggtgatc
     4801 gacggtcgcg gctggcgcac gggcgccttg gtcgagaaac gcgagattcc gggctactac
     4861 ctggcgatca cccagtacgc cgaggaactg ctttcggcgc tcgacggact tcctggctgg
     4921 cccgagcgcg tgcgcacgat gcaggcgaac tggatcggca agagcaccgg cgtgcgcttc
     4981 gccttcccct acgaagtcga tggccatccc gagaaactct gggtcttcac gacgcgcgcc
     5041 gatacgatca tgggtgtcac cttctgcgcc gtcgccgccg agcacccgct ggcgacgcgc
     5101 gccgcgcgcg acaacccgga actcgcggcc ttcatcgagg aatgcaagcg cggctcgacg
     5161 atggaagccg acgtcgcgac gatggagaag aagggcatgg cgaccggctt ttcggtgcgc
     5221 cacccgctga ccggcgccag cgtcccggtg tgggtcggca actacgtgct gatgggctac
     5281 ggagagggcg ccgtgatggc cgtgccggcg cacgacgagc gcgacttcca tttcgcgaag
     5341 cagtacgggc tgccaatcaa gccggtaatc gccgtcggcg accaggcgtt ctccgaagaa
     5401 tcctggcagg agtggtacgc cgacaaggta ggcggcatct gcatcaattc cggcaagtac
     5461 gacggcctcg gctatgaggc ggcgagcgat gccatcgccg gcgacttggc cgttcgcgac
     5521 ctcggcaaca agcaggtgca atggcgcctg cgcgactggg gtgtctcgcg ccagcgctac
     5581 tggggctgcc cgattccgct gatccactgc gcccaatgcg gcatcgtgcc ggtgcctgac
     5641 gagcagctgc cggtggtcct gcccgaggac tgcgtgcccg acggctcggg caatccgctc
     5701 aacaagcgcg aggacttcct gcattgcgcc tgcccgaaat gcggcggcga ggcacggcgc
     5761 gagaccgaca cgatggacac cttcgtcgat tcctcgtggt actacgcccg ctactgcgcg
     5821 cccgacaacg ccgaggcgat ggtcgacgcg cgcaccaact actggatgcc ggtcgaccag
     5881 tacatcggtg gcatcgagca cgcaatcctg cacctgctct actcacgctt ctggaccaag
     5941 gtcatgcgcg atctcggcct ggtcacctac gatgagccct tcgccaacct gctgacgcag
     6001 ggcatggtgc tgaaccacat cttctcccgg cgcaacgcca agggcggcat cgagtacttc
     6061 gcgcccgacg agatcgacgt aatcaccgac gaggccggcc gaatcaccgg cgcgacgctc
     6121 aaggccgacg gcagcgcgat cgactacaac ggcatcggca cgatgtcgaa gtcaaagcgc
     6181 aacggcgtcg acccacagag cctgatcgac cagtacggcg cagacacggc gcgtctgttc
     6241 atgatgttcg ccagcccgcc cgaacagacg ctcgaatggt ctgacgccgg cgtcgaaggc
     6301 gcgcaccgtt ttctcaaacg gctgtggaag gtcgcccaca cccacgtcgg cggcggcatc
     6361 gttgcagcct gcagcacgag cagcggcctc gacgagggtc agaaggactt gcggcgcaag
     6421 ctgcaccaga cgattgccaa ggtcgccgac gactacgg
//
LOCUS       DATDAA010000208         6108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_504836,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000208 DATDAA010000000
VERSION     DATDAA010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504836"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..359
                     /locus_tag="VIS73_06660"
     CDS             72..359
                     /locus_tag="VIS73_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY5762871.1"
                     /translation="MPDDPPAFEGFAKRSRAAGYNDVVERVWPPATIVDTHTHPFALH
                     ALVVRGEMWLTAAGTERHLVPGDEFSLECDEPHAERYGEAGATYWVARRNR"
     gene            complement(391..2562)
                     /locus_tag="VIS73_06665"
     CDS             complement(391..2562)
                     /locus_tag="VIS73_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY5762872.1"
                     /translation="MLKGDTWGGLAAMLVALPAAIAFGVTIYAPLGGGLAAQGALAGI
                     LGAMALGLIAGLLGGTPRLISAPCAPAAAVLSALAVQYAEAGMGAAAAVLTLTLIGLL
                     AGALQIGLGAARIGSLIKYMPYPVVSGYLTGVGLIIIASQTPKLLGIALPQGFFAAIV
                     APETWNWAAIAVGSVTIASMQLAPQITKRAPPAVIALGAGTLAHLALGLVDASLWRLD
                     GNPLVVGTIAGEGGEVWAKATAHFRAFTHLGLDSIVEVLVPALTLAVLLSIDTLKTAV
                     VLDAMTHSRHDSNRELIGQGAGNIGASLLGGVPGAGTMGPTLINLAAGAKTKRSGLFA
                     GGFTLIAFLLLAPVIAWVPVAALAAILIVVGLRMIDRQSLGFLFNPSTRFDFMVIAAV
                     VAVALGKSLIAASAAGIVFAILIFLREQSRATVVRAKVEGGAVHSKRFRNAAQSEVLK
                     AADEQTVILELQGSLFFGTAHQLYSAIEPEIGRRRYVILNMRRVNSIDVSASHMLERL
                     EDMLQDAGGRLVFAELPRDLPSGVKLKKYLKSVGLVGNRGAVAFRRLDDALEWIEAET
                     LATASLPDEETALELAELEIFRGRQPETLVSLAGFMTVRRYAVGERIFSVGDGGRDLY
                     LIRRGSVRLDVPMRKKDRHHLGTFGRGDFFGEVSFLDGSIRAVDATAVSEAELYVLTR
                     EAFELLSIDHKAASLNIVEGAARILAARLRAAITELRAWRS"
     gene            2901..3488
                     /locus_tag="VIS73_06670"
     CDS             2901..3488
                     /locus_tag="VIS73_06670"
                     /inference="COORDINATES: protein motif:HMM:NF028053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PliI family lysozyme inhibitor of I-type
                     lysozyme"
                     /protein_id="HEY5762873.1"
                     /translation="MNDNLKGASFLLVLLASMSASLHAEAPSRPFFAQQVLQGVTFQI
                     ESPNNSSINKVRVRAQVGKKTIGEHEVEADGAIIGVEVADLNADGFPEIYVYVTSTGS
                     GSYGSLIAFASNRNRSITPITLASLEDEDAAVTKGYMGHDKFLVGEAALLRRFPIYRE
                     GDANSKPTGGMRQIQYRLKAGEAGWVLRKDRVVEF"
     gene            complement(3644..4138)
                     /locus_tag="VIS73_06675"
     CDS             complement(3644..4138)
                     /locus_tag="VIS73_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762874.1"
                     /translation="MPKSNRPTGETAFAERTPLAALGVVLGLFFGGGALGLWLADQVA
                     SESAVAALVSFLALPAAFMLGFVLWAGAALPGAWRRFQTMRRAKVSGVPADLLPVAVP
                     PGAFAFVPAALIVNAGAGLLVGMLASDVSSYGTWVLYLLIGAAYGITCWQLAKRGLLA
                     FPNE"
     gene            complement(4122..5045)
                     /gene="ylqF"
                     /locus_tag="VIS73_06680"
     CDS             complement(4122..5045)
                     /gene="ylqF"
                     /locus_tag="VIS73_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEY5762875.1"
                     /translation="MPIQWFPGHMNSARRKAAETMARVDVVIEVVDARLPEASANPMI
                     RELAGARQRPRLKVLNKADLADAEATRAWLAYFKQQPGVTAVALSCKSAADVARIPNL
                     CKQLAPHRNDGTKPLRLMVMGVPNVGKSTLMNALLKKRVAAVGDEPAVTKSQQSLQIG
                     PDTWLTDTPGLMWPKIAHDSDGYMLAASHAIGVNAVIEEEVATFLGGLLLARYPQAVA
                     ARYKLKDDEVAAMDGVGLIEIVAKKRGVFRKLRGGGNEPDREKASLILLGDYRSGALG
                     RISLETPETRAAMLATAVPLTPAPEGDAEVE"
     gene            5137..5997
                     /locus_tag="VIS73_06685"
     CDS             5137..5997
                     /locus_tag="VIS73_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY5762876.1"
                     /translation="MTKPRNTAKAPPDIAAELSLRPDQSTELLQSLHILTRDGRLNQD
                     SRRKLKQVNHLVQFIEPLLDSTYTARGATTLVDHGAGKSYLGFLLYDLWAKRHNDAQV
                     IGVETRADLVESSRALAERLGFERMRFLNIAAAEAVDSEVLTGPIDVVTALHACDTAT
                     DDAIRFALERQARFVVLVPCCQAEVARHLREGKQASLGATALSELWRHPIHTREFGSL
                     VTNTLRCLLLEAHGYRLSVTELVGWEHSMKNELIVARREGPPRGDARARIAAILDALQ
                     LPALAARFNY"
BASE COUNT         1097 a         2100 c         1948 g          963 t
ORIGIN      
        1 tgccgctgaa ttcgcacaca aaatagccac cccagataac ggtacccatc gccgaacaga
       61 ggttgccagt aatgccagac gacccgcccg ccttcgaagg atttgccaaa cgctcgcgcg
      121 cggctggcta caacgacgtc gtcgagcgcg tctggccacc tgccaccatc gtcgacacgc
      181 acacacaccc gttcgcactg cacgccctgg ttgtccgcgg cgagatgtgg ctcacggcag
      241 cgggaacgga acgccacctg gtgccgggcg acgagttctc gctcgaatgc gacgaaccgc
      301 atgccgaacg ctacggcgag gcaggcgcga cctactgggt cgcacgacgc aaccgttaga
      361 tgggcagcaa cccgccaacg cgtcgcgcga ctaggagcgc caggcccgca actcggtaat
      421 cgcggcgcgc agccgtgccg cgagaatgcg ggccgcaccc tcaacgatgt tgagcgacgc
      481 cgccttgtgg tcgatcgaca ggagttcgaa ggcctcgcgc gtcagcacgt aaagctctgc
      541 ctcgctgacc gccgtggcgt ccaccgcgcg gatgctgccg tcgagaaagg acacctcgcc
      601 gaagaagtcg ccgcggccga aggtgccgag atggtgacgg tccttcttgc gcatcgggac
      661 gtcgaggcgc accgaccctc tgcggatcag gtacagatcg cgcccgccgt cgccgacgct
      721 gaagatgcgt tcgccgaccg cgtagcggcg cacggtcatg aagccggcga gcgacaccag
      781 cgtctccggc tgccggccgc ggaagatctc cagctcggcg agttcgagcg ccgtctcctc
      841 gtcgggcagt gaggcggtag ccagcgtctc ggcctcaatc cattcgagcg cgtcatcgag
      901 ccggcggaac gccaccgcgc cacggttgcc gaccaggccc accgacttca ggtacttctt
      961 cagcttgacg ccgctcggca gatcgcgcgg cagctcagcg aaaaccagcc gcccgccggc
     1021 gtcctgcagc atgtcttcca ggcgttcgag catgtggctg gccgacacat caatcgaatt
     1081 cacccgccgc atgttgagaa tcacgtagcg gcggcgaccg atttccggct cgatcgccga
     1141 atagagctgg tgcgcggtgc cgaagaacag gctgccctgc aattcgagga tcaccgtctg
     1201 ctcgtcggcg gccttgagca cctcgctctg cgcggcattg cggaaacgct tcgagtggac
     1261 tgcgccgcct tcgaccttgg cgcggactac ggtcgcccgc gactgctcgc gcagaaagat
     1321 caggattgcg aagacgatgc cggccgccga cgcggcgatc aggctcttgc caagtgccac
     1381 cgcgacgacg gcagcgatga ccatgaagtc gaagcgcgtc gaaggattga agaggaaacc
     1441 caggctctgg cgatcgatca tgcgcaggcc gacgacgatc aggatcgccg ccagcgccgc
     1501 caccggcacc caggcgatca ccggcgccag cagcaggaag gcaatcagag taaaaccgcc
     1561 ggcgaacaga cccgagcgct tggtcttcgc gccggccgcc aggtttatca gtgttgggcc
     1621 catcgtcccc gcgcccggca cgccgccgag cagcgaggcg ccgatgttgc cggcaccctg
     1681 cccgattaac tcgcggtttg agtcgtggcg cgagtgcgtc atcgcgtcga gcaccactgc
     1741 cgtcttcagc gtatcgatcg acagcagcac cgcgagcgtc agcgccggca ccagcacctc
     1801 gacgatggag tcgagcccga gatgcgtgaa ggcgcggaag tgcgccgtcg ccttcgccca
     1861 cacttcgccg ccctcgccgg cgatcgtgcc gaccaccagc ggatttccgt cgagccgcca
     1921 tagcgaggcg tcgacaagac cgagcgcgag atgggcgagc gtaccggcgc ccagcgcgat
     1981 gaccgccggc ggcgcgcgct tcgtgatttg cggtgccagt tgcatcgatg cgatcgtcac
     2041 gctgccgacg gcaatcgccg cccagttcca ggtttccggc gcaacgatcg cggcgaagaa
     2101 cccctgcggc agcgcgatgc cgagcagctt cggcgtctgg ctggcgatga tgatcaggcc
     2161 gacgccagtc aggtaaccgc tgaccaccgg gtagggcatg tacttgatca ggctgccgat
     2221 tcgcgccgcg ccgaggccga tctgcagtgc gccggcgagc agcccgatca gggtcagggt
     2281 cagcaccgcc gccgccgcgc ccatccccgc ttcggcgtac tgaaccgcca gcgcggacag
     2341 gaccgccgcc gccggcgcgc agggcgcgct gatcaatcgc ggcgtaccgc cgaggaggcc
     2401 agcaatcagc cccagcgcca tcgccccgag aatgccggcc aaggccccct gtgccgccag
     2461 tccgccgccg agcggcgcgt agatcgtcac accgaaggcg atcgccgccg gcagcgcgac
     2521 cagcatcgcg gccagcccgc cccaggtgtc gcccttcagc atttcccgat ctgctgcgct
     2581 gcgcatcaac gcggccccct cggactcgtc gccagtcccg ccattgtggg cgagccgaac
     2641 aggaaggcaa ggcggagccc cccgctgacg ccgtcttcct gcttcaatgt gtggcgcccg
     2701 gctgatgcct cgatcgtatc ggcccgcaga tgtcggccag ctgctgccgt tgatctactc
     2761 gctgactaat gtcgggttcg tccaaatagc ggaattgcca caacgtttgt cggcttagac
     2821 gcgcttgctt agacggcagt tttgacgtct aagttacgtt gggcatttat ccctctttgg
     2881 cgccaatggg agtgatgaag atgaatgaca atctgaaggg agcatcgttt ctcctcgtct
     2941 tgctagcttc gatgtcggca agcctgcacg ccgaagcccc gagccggccg ttcttcgcgc
     3001 agcaggtcct gcaaggggtg accttccaga tcgagagtcc caacaacagc tcgatcaaca
     3061 aggtcagggt gcgcgcgcag gtgggcaaga agacgatcgg cgagcacgag gtcgaggccg
     3121 acggcgcgat catcggcgtc gaggtggcgg atctcaacgc cgacggcttc ccggaaatct
     3181 acgtctacgt cacctccacc ggaagcggct cctacggctc tctgatcgct ttcgcatcga
     3241 atcgcaaccg gtcgatcact ccgatcacgc tagcgtctct agaggacgag gacgcggcgg
     3301 tgacgaaggg ctacatgggc catgacaagt tcttggttgg cgaagccgcc ttgttgcgcc
     3361 gcttcccgat ttaccgggag ggcgacgcca actcgaagcc gactggcggc atgcggcaga
     3421 tccagtacag gctgaaagcc ggcgaggccg gatgggtgct tcgcaaagac cgtgtggtgg
     3481 agttctgacg ctgcaggtca gaagtgacac gatgcccaac gaccggttcc agtcgacagc
     3541 tcgccaatga accggggcgt tgaggcggct ctggattggt tacaggccgt tcgcgcaaaa
     3601 aggtatcgaa cgtctcattt gggtcggtag cgagccctcg ctgctattcg ttcggaaacg
     3661 ccagcagccc gcgcttggcg agctgccagc aagtgatccc gtaagcggcg ccgatcagta
     3721 agtacagcac ccaagttccg taagagctga catcgctggc gagcatgccg accagcaggc
     3781 cggcaccagc gttgacgatc aacgcagcgg gtacgaatgc gaaagcgccg ggcggcactg
     3841 ccaccggcag caagtcggcc ggaacgccgc tgaccttcgc gcgccgcatt gtctggaaac
     3901 gacgccaggc gcccggcaat gccgcgccgg cccataacac gaagccgagc atgaaggcgg
     3961 cgggcagcgc aaggaagctg acgagcgcgg cgactgctga ctcggaagca acctgatcgg
     4021 cgagccacaa gccgagggcg cccccgccga agaacaggcc caggacgacg cccagcgcgg
     4081 ccagcggcgt gcgctcggca aacgccgttt cgccggtggg cctattcgac ttcggcatcg
     4141 ccttccggtg cgggggttaa tggcaccgcc gtcgccagca tcgccgcccg tgtctccggc
     4201 gtctcgaggc tgatccggcc gagcgcgccg ctgcggtagt cgccgagcag gatcagcgag
     4261 gccttctcgc ggtcgggttc gttgccgccg ccgcgtagct tgcggaagac gccgcgcttt
     4321 tttgcaacga tctcgatcag gccgacaccg tccatcgccg cgacctcatc gtccttcagc
     4381 ttgtagcgtg cggcgactgc ctgcgggtaa cgggccaaca agaggccgcc gaggaaggtg
     4441 gcgacctcct cctcgatcac cgcgttgacg ccgatcgcgt ggctcgcggc gagcatgtag
     4501 ccgtccgaat catgggcgat cttcggccac atcaggccgg gcgtgtcggt gagccaggtg
     4561 tccgggccga tctgcaagct ctgctggctc ttggtcaccg ccggctcatc gccgacggcg
     4621 gcaacgcgct tctttagcag tgcgttcatc aaggtcgatt tgccgacgtt gggcacgccc
     4681 atcaccatca gccgcagcgg cttggtgccg tcgttgcggt gcggcgccag ttgcttgcag
     4741 aggttgggaa tacgcgcgac gtcggcggcc gacttgcacg acagcgccac cgcggtcacg
     4801 cccggctgct gcttgaagta agcgagccag gcccgggtag cctcggcgtc ggcgaggtcg
     4861 gccttgttca gcaccttcag tcgcggccgc tgccgtgcgc cggccagttc gcggatcatc
     4921 gggttggcgc tggcctcggg cagccgcgca tcgacgacct cgatcaccac gtcgacgcgc
     4981 gccatcgtct cggcggcctt gcggcgcgcg gagttcatgt gaccggggaa ccactggatc
     5041 ggcatcggca gcttcaagtc ggggaaggcg cgcattatcc catcgctacc gcgagggtca
     5101 ccgccgcgat gcctcaggcc ggcttaagat tcgcccatga cgaaaccccg aaacactgcg
     5161 aaagcgccgc cagacatcgc cgccgaactg agcctgcggc ccgaccagtc gaccgaactt
     5221 ttacagtcgc tgcacatcct gacccgcgac ggccgcctca atcaggacag ccggcgcaag
     5281 ctcaagcagg tgaaccacct cgtgcagttc atcgaaccgc tgctcgattc gacctacacc
     5341 gcgcgcggcg cgacgacgct ggtcgaccac ggcgccggca agtcctacct cggcttcctg
     5401 ctctacgatc tgtgggcgaa acgccacaac gatgcgcagg tgatcggcgt cgaaacccgc
     5461 gccgatctgg tcgagtcgtc gcgcgcactc gccgagcgac tcggcttcga gcgcatgcgc
     5521 ttcctcaaca tcgcggctgc cgaggcggtc gactcggaag tgctcaccgg accgatcgac
     5581 gtagtcacgg ccctgcacgc ctgcgacacc gcgaccgacg acgccatccg cttcgcgctc
     5641 gaacgccagg cccgcttcgt cgtgctggtg ccctgctgcc aggcagaagt cgcgcgccat
     5701 ctgcgcgagg gcaagcaagc gagcctcggg gctacggcac tgtcggagct gtggcggcac
     5761 ccaatccaca ctcgcgaatt cggcagtctg gtcaccaaca cgctgcgctg tctattgctc
     5821 gaagcgcacg gctatcgcct ctcggtgacc gagctggtcg gctgggagca ctcgatgaag
     5881 aacgaactga tcgtcgcccg ccgcgagggg ccgccgcgcg gcgacgcccg cgcgcgtatt
     5941 gctgccatac tcgacgcact gcagctgccg gcactcgccg ctcgtttcaa ctactgaaag
     6001 accttcatga acgactcaac tccccatcgg ctcgctgtcc tcatcgacgc cgacaacgtc
     6061 tcggcctcgg tcctcgacgg cctgctcgcc gaagtcgcca aatacggc
//
LOCUS       DATDAA010000209         2377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_50233,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000209 DATDAA010000000
VERSION     DATDAA010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50233"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..732
                     /locus_tag="VIS73_06690"
     CDS             244..732
                     /locus_tag="VIS73_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY5762877.1"
                     /translation="MGDDMAEHVDVRLDLKGLSCPGPILGAKKMVDSLEEGQILLLIS
                     DCPGTQDDLYAWADNTGNSVLRVDRFADGSNGYYVRRGRGKLPAANVTLDMRGAVCPG
                     PILEAKRLVNGMATGEVLKLISNCPGTRDDVEGWTRNTGLDLLNVVQVDSSDFEFYIR
                     KP"
     gene            741..1415
                     /locus_tag="VIS73_06695"
     CDS             741..1415
                     /locus_tag="VIS73_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762878.1"
                     /translation="MAVRVKTHWFQEGRNRGPEQQASVIAAAIWKTGVHGLDGLRKAK
                     FSVDVGAPYIAVLTEFLLFLATAADRWAYLSVRDGSEQDGVWREGFTGAMARRLADIY
                     RDNMDNLVGPFEPGARFGTLSVPQPGGYAEFFVAALNERMAEYADFGFDESGPDFAFM
                     RYFGSCIEEVIADPEDRKWVLDQVMTVQAPEALEIVARGMRGIVGGEAGEKSTEPHRP
                     RGVAGD"
     gene            1415..2374
                     /locus_tag="VIS73_06700"
     CDS             1415..2374
                     /locus_tag="VIS73_06700"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY5762879.1"
                     /translation="MAADDARGLPPGNPFALDDEAAYRRWREWKLDHAPRRVEDLLVP
                     IADLAAPTATELAALTRVLRRANMALYASVAAQNREEKQLPMALGARLGLTRLDANWL
                     ADEDGVSSITPAAADSGAPRPDFIPYTTQPIRWHTDGYYNPPERTIRAMILHCVRPAS
                     KGGANALLDHEIAYIMLRDESPEWIRALSAPDAMTIPARRNDDGEARSAQSGPVFSVD
                     AETGDLHMRYTARTRSIVWKGDAATAAAVARLSSLLDGDARSAPHVFRLRQESGMGLV
                     CNNVLHDRAAFADGAGSRQRLLYRARYYDRIAPTVGAWRIDSE"
BASE COUNT          407 a          766 c          820 g          384 t
ORIGIN      
        1 ggtcgcggtc ggttcgggcg agcagcggct ggcgggttac ggtgtggtgg tccacgacct
       61 cgggccacgg cactcgctgg ttctgctgga gcaagggatc ggtccgggac gccattacgg
      121 ctgcggcctg ttcgtgcatc acaagctgat cgaggggctg gacgcctacc ctgaataggg
      181 cgcaacgatt gattcgaacc gggccaaccg gccttgccgg cggtaccggg aaggagagac
      241 atcatgggag atgacatggc agagcacgtc gacgtcaggc tggacctcaa gggcctcagt
      301 tgccccgggc ccatcctcgg cgccaagaag atggtggatt cgctggaaga ggggcagatt
      361 ctgctgctca tctccgattg ccctgggacg caggacgatc tctacgcttg ggccgataac
      421 accggcaaca gcgtcctccg cgtcgatcgc ttcgccgatg gctcgaacgg ttactacgtg
      481 cggcgcggcc gcggcaagct gccggcagcc aacgtcacgc tcgacatgcg cggcgccgtc
      541 tgcccggggc cgattctgga agccaagcgg ctggtaaacg gcatggccac cggcgaggtg
      601 ctcaagctga tcagtaactg cccggggaca cgcgacgacg tcgaaggctg gacgcgcaat
      661 accggcctcg acctgctcaa cgtggtgcag gtcgattcga gcgatttcga gttctacatt
      721 cgcaagcctt aggaattccc atggccgtcc gggtcaagac ccactggttt caggagggcc
      781 gcaaccgcgg gcccgagcag caggcgagcg tgattgccgc ggcaatctgg aagaccgggg
      841 tccacgggct cgacgggctg cgcaaggcca agttttcggt cgacgtcggt gcgccctaca
      901 tcgcggtgct gaccgagttt ttgctgttcc tggcaacggc cgccgatcgc tgggcctacc
      961 tgtcggtgcg cgatggcagc gagcaggacg gcgtgtggcg cgaaggcttt accggagcaa
     1021 tggccaggcg tctcgccgac atctatcgcg acaacatgga taacctggtc gggccgttcg
     1081 agccgggtgc gcgcttcggc acgctgagcg tgccgcaacc cggcggctac gcagagttct
     1141 tcgtcgccgc gctcaacgaa cgcatggccg agtacgccga cttcggcttc gacgagagcg
     1201 gccccgattt cgccttcatg cgttatttcg gcagctgcat cgaagaggtc atcgccgatc
     1261 ccgaggatcg caagtgggtg ctcgatcagg tcatgacggt gcaggcgccc gaggcgctgg
     1321 agatagtcgc gcgcggcatg cgcgggatcg tcggtggtga ggccggcgag aaatccacag
     1381 agccgcaccg gccgcggggc gtcgccggcg attgatggca gccgatgacg cccgcgggct
     1441 gccgcccggc aacccgttcg cgctcgacga cgaggccgcc taccggcgct ggcgcgaatg
     1501 gaagctcgac cacgcgccgc gccgcgtcga agacctgctg gtgccgatcg cagatctggc
     1561 agcgccgacg gcgaccgagc tggccgcact gacccgtgtg ttgcgacgcg ccaacatggc
     1621 gctctatgcc tcagtggctg cgcagaaccg cgaggagaag cagctgccga tggcgctcgg
     1681 cgcccggctt ggccttaccc gactcgacgc caactggctg gccgacgagg atggcgtcag
     1741 cagcatcacg ccggcagcag cggatagtgg cgcccctcgt ccggatttca ttccctacac
     1801 cacgcagccg attcggtggc acaccgacgg ctattacaat ccgcccgagc ggacgatccg
     1861 ggcgatgatt ctccactgcg tgcgacccgc cagcaaaggc ggcgccaacg cactgctcga
     1921 ccacgagatc gcctacatca tgctgcggga cgagagcccg gaatggattc gcgcgctgtc
     1981 ggcgcccgat gccatgacga taccggcacg ccgcaatgac gacggcgagg cgcgttcggc
     2041 gcagtcgggt ccggtgttct cggtcgatgc cgaaaccggc gacctgcaca tgcgctacac
     2101 cgcgcggacc cgcagcatcg tctggaaggg tgacgcggcg acggccgccg cggtggcgcg
     2161 gctgtcgtca ctgcttgacg gcgacgcgcg atcggcgccg catgtctttc gcctgcgtca
     2221 ggaaagcggc atgggcctcg tctgcaacaa cgtattgcac gaccgggcgg cgtttgccga
     2281 cggggccggc agtcggcagc ggcttctcta ccgggcgcgc tattacgatc gaatcgcgcc
     2341 gacggtgggt gcctggcgga ttgattccga ataaccc
//
LOCUS       DATDAA010000210         3600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_470186,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000210 DATDAA010000000
VERSION     DATDAA010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470186"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VIS73_06705"
     CDS             <1..339
                     /locus_tag="VIS73_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY5762880.1"
                     /translation="MTTTANAGGCHNLLVRPGRRDLDAMIVAMGRGLLVTELLGHGVN
                     YVNGDYSRGAAGYWVENGRIAHPVEEITIAGNLAEMFAGVVETGADTIIRGSKQTGSW
                     WIDRMTVAGA"
     gene            complement(357..433)
                     /locus_tag="VIS73_06710"
     tRNA            complement(357..433)
                     /locus_tag="VIS73_06710"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(397..399),aa:Ile,seq:cat)
     gene            complement(476..2779)
                     /gene="rpoD"
                     /locus_tag="VIS73_06715"
     CDS             complement(476..2779)
                     /gene="rpoD"
                     /locus_tag="VIS73_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY5762881.1"
                     /translation="MSKGKTATTSARSSTAKPAAKPATSSGKTAAKPAAKTASTAKAA
                     PKATAARSAAKPAAKPSTAAAAKRLPAKAAEKPSVAKPKAAPAKAKPAPAKAAPAAAP
                     AKPAKAARAPSKSKGARAKARDRIAELANMAPAASPLDAETRKTRLKSLIKLGKERGY
                     LTYAEINDHLPDELVDGEQIESIISTFSDMNIKVYDEAPAAEDLLMADATPSSSDDEE
                     VEEEAEQALSTVDSEFGRTTDPVRMYMREMGSVELLTREGEIEIAKRIEEGLKHMILA
                     IAACPTTIAEIVEMADKVVREEMRIDEMIDGLIDPNAVEEDPKPAANSAAAREASNDS
                     DSDSDDDSDDSDDDEDDGASAAAASLAQMRAEALERFAVIRTAHQKAQGILAKKGSQD
                     KSYIKLQDQISNEMMHIRFSSKVIERLCDSVRGMIEGVRAAERLIQEICVFKVRMPRQ
                     HFIKTFPGSETDLGWVDREIASGARSYGNLLGHHAPYIKEAQQVLIDLQNRLGISLKE
                     LKDINKQMSTGEAKARRAKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKA
                     VDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQILQE
                     TGQEPDPATLAEKMEMPEDKIRKILKISKEPISMETPIGDDDDSHLGDFIEDTATPAP
                     LDAALQSSLGDATKQILDSLTPREAKVLRMRFGIEMSTDHTLEEVGKQFDVTRERIRQ
                     IEAKALRKLRHPTRSERLRSFLDQGNS"
     gene            complement(2858..>3600)
                     /locus_tag="VIS73_06720"
     CDS             complement(2858..>3600)
                     /locus_tag="VIS73_06720"
                     /inference="COORDINATES: protein motif:HMM:NF019883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY5762882.1"
                     /translation="ADDPDSFVRQHGADAFRGFVRRALPLSEYLVQELARRHPPDSAE
                     GRVALLHDAKPLLGKIASPAYRLQLVKRLGDITETTPGEIESLCGLRPVAALPAVKRV
                     VRATPSLARSVARLILRQPALAAQLPDEALPGGTEGGALRALRDAAISGQTPTTAALI
                     ERLHGVADPRWLEALSAEVISHPLDDDACQTEFADALARLRASHRQSALRQLAERARQ
                     GTLSDAEKAEYLTLLRERTAPPSEEAGD"
BASE COUNT          497 a         1129 c         1208 g          766 t
ORIGIN      
        1 atgaccacca ccgccaacgc cggcggctgc cacaacctgt tggttcgccc cggccggcgc
       61 gacctcgacg ccatgatagt cgccatgggt cgcggactgc tcgtcaccga attgctcggc
      121 cacggcgtca actacgtcaa cggcgactac tcgcgtggtg ccgctggcta ctgggtggag
      181 aacggccgga tcgcccatcc cgtcgaggaa atcaccattg ccggcaattt ggccgaaatg
      241 ttcgccggcg tggtcgagac cggcgccgac acgatcattc gcggctcgaa acagacgggt
      301 tcgtggtgga tcgaccggat gaccgtcgcc ggcgcctaag ccggccgaac gtttcttggt
      361 gggccgtgtt ggactcgaac caacgaccaa gggattatga gtcccctgct ctaaccgact
      421 gagctaacgg cccggcgatg ctggtggcgc ctggcggcgc caccttttct gctgactagc
      481 tgttgccctg atcgaggaag ctgcgcaggc gctccgaacg ggtcgggtga cgcagcttgc
      541 gcagcgcctt ggcttcgatc tggcgaatcc gctcgcgggt cacatcgaac tgcttgccga
      601 cttcctcaag cgtgtggtcc gtgctcatct cgatgccgaa acgcatgcgc aggactttcg
      661 cctcgcgcgg tgtcagcgag tcgaggatct gcttggtggc atcgccgagg ctcgactgca
      721 gcgccgcgtc aagcggagcc ggcgtcgcgg tgtcctcgat gaagtcgccg aggtgcgagt
      781 cgtcgtcgtc gccgatcggc gtttccatcg agatcggctc cttggagatc ttgaggatct
      841 tgcggatctt gtcctcgggc atctccatct tctcggcgag cgtcgccgga tcgggttcct
      901 gtccggtttc ctgaaggatc tggcgggaga tccggttcat cttgttgatc gtctcgatca
      961 tgtgcaccgg gatgcggatg gtgcgcgcct ggtcggcaat cgagcgggtg atcgcctggc
     1021 ggatccacca ggtggcgtag gtcgaaaact tgtagccgcg acggtattcg aacttgtcca
     1081 ccgccttcat cagtccgatg ttgccttcct gaatcaggtc gaggaactgc aggccgcggt
     1141 tggtgtactt cttggcaatc gagatcacca ggcgcaggtt tgcctcggtc atttcgcgct
     1201 tggcgcggcg cgccttggct tcacccgtgg acatctgctt gttgatgtcc ttgagttcct
     1261 tgagcgagat gcccagacgg ttctgcaggt cgatcagcac ctgctgcgct tccttgatgt
     1321 agggcgcatg gtggcccagc aggttgccgt aggaccgtgc gcccgatgcg atctcgcgat
     1381 cgacccaccc gaggtcggtt tcgctgccgg ggaaggtctt gatgaagtgc tggcgcggca
     1441 tgcgcacctt gaagacgcag atttcctgga tcaatcgctc ggcggctcgc accccttcga
     1501 tcatgccgcg aacgctatcg cacaggcgct cgatcacctt cgagctgaag cggatgtgca
     1561 tcatctcgtt ggaaatttga tcctgcagct tgatgtagct cttgtcctgc gaacccttct
     1621 tggcgaggat tccctgcgcc ttctggtgcg ccgtgcggat caccgcaaag cgttcgagtg
     1681 cctcggcgcg catctgcgcc agcgatgccg cggcagctga ggcgccatca tcctcgtcgt
     1741 catcgctgtc gtcactgtcg tcatcgctgt cgctgtcgct gtcgttgctc gcttcccgcg
     1801 cggcagcgct gttggctgcc ggtttgggat cttcctcgac ggcattcggg tcgatcaggc
     1861 cgtcgatcat ttcgtcgatg cgcatttcct cacgcacgac cttgtcggcc atttcgacga
     1921 tttcggcgat ggtcgtcggg caggccgcga tcgccaggat catgtgcttg aggccttcct
     1981 cgatccgctt ggcgatctcg atttcaccct cgcgcgtcag cagttcgacc gaacccattt
     2041 cgcgcatgta catgcgtacc ggatcagtgg ttcggccgaa ctcggagtcc actgtcgaca
     2101 gcgcctgctc ggcttcttcc tcgacttcct cgtcatcgga cgagcttggg gtcgcgtcgg
     2161 ccatcagcag gtcttcggcg gcgggcgcct cgtcgtagac cttgatgttc atgtcgctga
     2221 aggtcgagat gatgctttcg atctgctctc catcgactag ctcgtcaggc aggtgatcgt
     2281 tgatctcggc gtaggtcaga tagccgcgct ccttgccgag cttgatcagg ctcttcagtc
     2341 gggtcttgcg ggtttctgcg tctagcgggg atgcggccgg cgccatgttc gccagttcgg
     2401 cgatgcggtc gcgtgccttg gcgcgggcgc ccttgctctt cgacggtgcg cgggcggcct
     2461 tcgctggctt ggctggggct gctgccgggg cggctttggc gggcgccggc ttggccttgg
     2521 ccggcgcagc tttcggtttg gctaccgacg gcttttcggc ggccttcgcc ggcaggcgct
     2581 tggcggctgc ggccgtcgac ggcttggcgg ccggcttcgc ggcgctgcgc gcggccgtgg
     2641 ccttgggcgc agcttttgcc gtcgatgcgg tcttcgccgc tggtttcgcg gcggtcttgc
     2701 ccgatgaagt ggccggcttg gcagcaggtt tggcagtgct gctgcgcgcg gaagtggtgg
     2761 cggttttgcc cttcgacatg aacgtccccg aggtggcgtg gcgaaaaact ctaaattata
     2821 tcagaaactt aaagcctgtc attgcggaaa tgctggctca gtcaccggct tcttcgctgg
     2881 gcggtgctgt gcgctcgcgc agcagcgtca ggtactccgc cttctccgcg tcgctgagcg
     2941 ttccctggcg ggcgcgctcg gcaagctggc gcagcgccga ttgccggtgg ctggcgcgca
     3001 gccgcgccag ggcgtcggca aattccgtct ggcaggcatc gtcgtcaagc ggatggctga
     3061 tgacttcggc gctcagggcc tccagccagc gcgggtcggc aaccccgtgc agtcgctcga
     3121 tcagtgccgc ggtggtcggc gtctggccgg aaatggccgc gtcacgtaag gcgcgcagcg
     3181 cgccgccttc ggtcccgccg ggcagcgcct cgtcgggaag ctgcgcggcg agcgccggct
     3241 ggcggagaat caggcgggcg acgctgcgcg ccagagaggg agtcgcccgg accacgcgct
     3301 tgacggcggg cagcgcggca acggggcgca gcccgcacag cgactcgatc tcgccgggag
     3361 tggtttcagt gatatcgccc aggcgtttga ccagttgcag ccgatacgct ggcgaggcga
     3421 tcttccccag caacggcttc gcgtcgtgga gcagggcgac gcgcccctcg gcgctgtcgg
     3481 gcggatggcg tcgcgccagt tcttgcacca gatactccga cagcggcagc gcgcggcgga
     3541 cgaagccgcg gaacgcgtcg gcgccatgtt gccgcacgaa gctgtccggg tcgtcggcct
//
LOCUS       DATDAA010000211         3843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_330261,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000211 DATDAA010000000
VERSION     DATDAA010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330261"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VIS73_06725"
     CDS             <1..324
                     /locus_tag="VIS73_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEY5762883.1"
                     /translation="GSLYGAIDGPWPGLLKLALGHDYIVWDKAGSIRYRKPGRSTLFA
                     EFAVDEAEIAWVRDAVRRDGECERNYTVELRDRGGVVHAIVERTVYIASKAHYAGKRP
                     PDGAR"
     gene            430..1017
                     /locus_tag="VIS73_06730"
     CDS             430..1017
                     /locus_tag="VIS73_06730"
                     /inference="COORDINATES: protein motif:HMM:NF022428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HEY5762884.1"
                     /translation="MFEALFDFTDPGWRNVVVWVVAALAIYILVLLLRMLRLSRTEAA
                     PSDSPSFDEIRQAKLQPYLDEGERSATAADTAVPFVEDRSDLAWREAPQRFAEQQVQA
                     ALEREVNALREEVDGLREAFSALREDMLKDLAALRAQQGVAAFYREAMSLAASGKPAE
                     DIAARCGIARAEADLVIALAQAQKPKSDDDREAYG"
     gene            1010..1798
                     /locus_tag="VIS73_06735"
     CDS             1010..1798
                     /locus_tag="VIS73_06735"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY5762885.1"
                     /translation="MAEESAAAAGEDSSELKRQLVQRAAVAAVLAAALLGGLAIFDRL
                     YQEAPPEPPLPRRMAAPPPAVSAPAVSEPAAAATAPTDTDGPTDIAVAGVPPPVPTPP
                     ATAAETLAPRAPAQTLRVLAAREPSAEVPALPEASEPAAPAAVPESSGAPEVPPPPVV
                     AAVPAAGVPEETGGPVRRSSRREPVATGLAHGYIVQAGVFNSPRRAEQLHDRLRRAGV
                     PVRLETRVQVGPFASREEAEAAREKLKSLGVESLLVPPRLPGNP"
     gene            1920..2648
                     /locus_tag="VIS73_06740"
     CDS             1920..2648
                     /locus_tag="VIS73_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762886.1"
                     /translation="MNKPVSTVVVTIDVPGVPAVPPGEMKAIFDRLVLAPCAGLGYAT
                     QEPGGLNLTLTLPPEGPADSALRRVGAACAEFDSQTTPVKTRALIHYGTVFRNEGTGG
                     RITYLGSAIRAAQTSLRRIAATSGIFASKDFTTHVTNFKAGTFGMEIHAGPEDARPVR
                     FEDRRKSGGGEVPGSDPQFQITLKKRLAEEIGPFAGPLVDNTKHSTMSAKELVAALSH
                     EIDNPESRQRFEVEMLAYIKARTR"
     gene            2753..3142
                     /locus_tag="VIS73_06745"
     CDS             2753..3142
                     /locus_tag="VIS73_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY5762887.1"
                     /translation="MPDSDCRDNAQMTLQRFPAITPWIAGAVLALLAASPAGAVDEDA
                     AVKLAKKGNCFKCHAVNKRKKAPAYAEVAEKYRSKPDPRRTLYLHITGEPIVKLAEGD
                     EPHAPPPSKSDAELYNLIDWILSRERK"
     gene            complement(3210..>3843)
                     /locus_tag="VIS73_06750"
     CDS             complement(3210..>3843)
                     /locus_tag="VIS73_06750"
                     /inference="COORDINATES: protein motif:HMM:NF023282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HEY5762888.1"
                     /translation="VNLTQSANAIFNNMPDDCLGRDRAEGGTFNLDVQWQPEENLTTF
                     AFAHYGLLTTKFAGRNWNSSGFVGLANSTNYNYLATQDQQDQTYGIGFKWIPSETTWD
                     ISGHYAFSHGRGSTSFATGSSVTGETSVPDSTSQSHAVQLYAKWNFSRAMTVRFNYLY
                     ETLKSYDWALVDPVTGQPYPTTVGSLTWTGQSAPKYENHVIGVSVALHSW"
BASE COUNT          681 a         1246 c         1244 g          672 t
ORIGIN      
        1 ggttcgctgt acggcgccat cgacgggccg tggccgggcc tgctgaagct tgcgctcggc
       61 catgactaca tcgtttggga caaggccggc tcgatccgct accgcaagcc gggccggagc
      121 acgctgttcg ccgagttcgc cgtcgacgag gccgagattg cttgggtgcg cgacgccgtc
      181 aggcgtgacg gtgaatgcga gcgaaactac acggtcgaac tgcgcgaccg cgggggcgtt
      241 gtgcacgcaa tcgtcgagcg caccgtctac atcgcgagca aggcgcacta cgcgggaaag
      301 cgaccgcccg acggcgcgcg atgagcctgt gatggcgcgc gtcgcggtga ttgcgaaacc
      361 ggccgcagcg ccgggaatga cgccctgtct ggcataatcg cgggccatga cgcgagcgct
      421 tttccgctga tgttcgaggc actatttgat ttcaccgatc cgggctggcg caatgtcgtc
      481 gtctgggtcg tcgcggcgct ggcgatctac attctggtcc tgctcctgcg catgctccgt
      541 ctttcccgga ccgaggctgc gccatccgat tcgccgagct tcgacgagat ccgtcaggcc
      601 aagctgcaac cctatctgga cgagggcgag cgcagcgcca ccgccgccga caccgccgtt
      661 ccctttgtcg aggatcgtag cgacctcgcc tggcgtgagg cgccacagcg tttcgccgag
      721 cagcaggtgc aggccgcgct cgaacgcgag gtcaacgcgt tgcgcgaaga ggtcgacggc
      781 ttgcgcgagg ccttttcggc gctgcgcgaa gacatgctca aggaccttgc cgcgctgcgc
      841 gcgcaacagg gcgtcgcggc cttctaccgt gaggcgatgt cactcgccgc atcgggcaag
      901 ccagccgagg acattgcggc gcgttgcggc attgcccgcg ccgaggccga cttggtgatt
      961 gcgttggcgc aggcgcagaa gcccaaatcc gatgacgacc gggaggccta tggctgagga
     1021 atccgcagcg gcggctggcg aagacagctc cgagctgaaa cggcagctgg tccagcgcgc
     1081 agcggttgcg gcggtactcg ccgcggcgct gttgggcggg ctggcgatct tcgatcgcct
     1141 gtatcaggaa gcgcccccgg agccgccctt gccacgccgg atggcggcgc ccccgcccgc
     1201 cgtgtcggcc cccgcggtat cggagccggc ggctgccgcg accgcgccga ccgatactga
     1261 cggcccgact gatatcgccg tcgccggcgt gccaccgccg gtcccgacac cgcccgcgac
     1321 cgctgcggaa acactcgcgc cgcgggcgcc ggctcagacc ctgcgcgtat tggcagcccg
     1381 tgaaccctca gccgaggtgc cagcgttgcc tgaggccagc gagccagcag cccctgccgc
     1441 tgtgcccgag agcagtggcg cgccggaagt gccgccgccg ccggtcgtcg ccgcggtacc
     1501 cgccgccggt gttcccgagg agaccggtgg cccggtgcgc cggtcttccc gccgcgagcc
     1561 ggtggcgacg gggctcgccc atggctacat cgttcaggcc ggcgtcttca acagcccgcg
     1621 gcgcgccgag caactccatg accgactgcg tcgggccggc gtcccggtgc gcctggagac
     1681 gcgcgtacag gtcggcccgt tcgcctcgcg cgaggaagcc gaagcggctc gcgaaaaact
     1741 caagtcgctc ggcgtcgagt cactgctggt tccgccgcgt ctgcccggaa acccatgacc
     1801 cgcggccacg ggagcggaac tagttgtttg actgagcgca gtcccgccgc gatactgccg
     1861 cgtgtttcgt agtgccataa gtccgtataa gaaaacccat cgagaaacga ggtgatgcct
     1921 tgaacaagcc cgtatcgacc gtcgtcgtaa ccattgatgt gccaggggta ccggccgtgc
     1981 cgccgggcga aatgaaggcc attttcgacc gattggtgct ggcgccctgc gcgggactcg
     2041 gttacgcgac acaggaaccc ggcgggctga atctgacctt gaccctgcct cccgaaggtc
     2101 cggccgattc ggccttgcgt cgcgtcgggg cggcctgcgc cgagttcgac tcgcagacca
     2161 cgccggtcaa gacacgggcg ttgattcatt acggcacggt ttttcgcaac gagggaaccg
     2221 gcggccggat cacctatctg ggatcggcca ttcgggctgc gcagacctcc ttgcggcgca
     2281 tcgcggcgac ctcgggcatt ttcgcgagca aggatttcac cacgcacgtc accaacttca
     2341 aggccggcac cttcggcatg gagatccacg cggggcccga ggacgcacgg ccggtgcgct
     2401 tcgaggaccg gcgcaagagc ggtggcggcg aggttccggg atccgacccg cagttccaga
     2461 taaccttgaa gaagcggctt gccgaagaaa tcgggccgtt cgccggcccg ctcgtcgaca
     2521 acaccaagca ttcaacgatg agtgccaagg aactcgtcgc cgcgctcagc cacgagatcg
     2581 acaatcccga atcgcgacag cgctttgagg tcgagatgct ggcctacatc aaggcacgca
     2641 cacgctgagg cattacggcc ggtcatcgga ggccgtggcg cgccaccgct gaaaaagact
     2701 atcccaggca tagggatggt cttgtcctcc tccgggcggg ataatgcgtt gcttgcctga
     2761 ctccgactgc cgcgacaacg cccagatgac attacagcgc tttcccgcca ttaccccatg
     2821 gatcgccgga gccgtgcttg cgttgctcgc cgcatcgccg gcgggcgccg tcgacgagga
     2881 tgccgccgtg aagctggcga agaagggcaa ctgcttcaaa tgccacgccg tcaacaaacg
     2941 gaagaaggct ccggcctacg ccgaggttgc cgagaagtat cgcagcaagc ctgatccgcg
     3001 ccgtaccctg tatctgcaca tcaccggcga gcccatcgtc aagctcgccg aaggcgatga
     3061 gccgcatgcg ccgcccccct cgaaaagcga cgccgagctc tacaacctga tcgactggat
     3121 cctgtcccgc gaacgcaagt gacgtatcaa caggctgccg aaaaagaaaa cggcccgtca
     3181 tcgacgggcc gttttgttct ttcgcgctgt taccagctgt gcagcgccac cgagacgccg
     3241 atcacgtggt tctcgtattt gggggctgac tgtccagtcc acgtcaagct tccaacggtc
     3301 gttgggtagg gttgcccagt gaccgggtca accaaggccc agtcgtagct cttgagcgtc
     3361 tcatacaaat aattgaagcg taccgtcatc gcacggctga aattccattt tgcgtagagc
     3421 tgtacagcgt gtgattggga ggtcgaatcg ggaaccgagg tttctccggt tacggatgat
     3481 ccggttgcaa acgaggtact gccgcgaccg tggctgaagg cataatgtcc gctgatatcc
     3541 caagttgttt cggagggaat ccacttgaag ccaattccgt aggtctggtc ctgctgatct
     3601 tgtgtggcca gatagttata gtttgttgag ttggcgagcc caacaaagcc actgctattc
     3661 caatttcgcc cggcaaactt tgtggtcagc aggccgtagt gggcgaaggc aaaagtcgtc
     3721 aggttttcct cgggttgcca ttggacgtcc agattgaatg tccctccctc ggcgcggtca
     3781 cgccccaggc agtcatctgg catgttgttg aatatggcat tggcagactg cgtgaggttg
     3841 acc
//
LOCUS       DATDAA010000212         7096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_85438,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000212 DATDAA010000000
VERSION     DATDAA010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85438"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VIS73_06755"
     CDS             <1..1036
                     /locus_tag="VIS73_06755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY5762889.1"
                     /translation="LDWNRRVFEAIRLLPATLVDTRGRGIATTLLGQPAAAPLAIAPT
                     GINGIYARDADLALARAAAAAKIPFCLSTVSNARLETIAAEAGGRLWMQLYVMKNRDI
                     ARDIIGRAEAAGYEALMLTTDANVFGHREWDKRNYRAPGELSWKNLLDVALHPRWALE
                     VMAPDGLPPFENIVDHMPAEAQSAKGGVAFIPQQFAPDVTWDEFAWIRRQWPRKLMIK
                     GILSAADARRAADAGCEAIVLSNHGGRQLESCVSPIDMLPDVVAAVGDRLEVYVDSGF
                     RRGNEIIKAIALGAKAVMVGRATLYGLAAGGQAGVQQALQILASETDRTLGQLGCNAL
                     DELGPEHLRR"
     gene            1130..1567
                     /locus_tag="VIS73_06760"
     CDS             1130..1567
                     /locus_tag="VIS73_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEY5762890.1"
                     /translation="MNTTDSSATSAWNLLFAAWLVATGSTLGALFFGEVMGLPPCLLC
                     WWQRIFMFPLAVLLPFGLFPYDSRMIRYGLVLAIPGALTALFHQLLVSGIVPETVKPC
                     TQGVPCSETVITWFGFLTIPLLSLAAFSILIALLIAAQIRSNS"
     gene            1564..2217
                     /locus_tag="VIS73_06765"
     CDS             1564..2217
                     /locus_tag="VIS73_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY5762891.1"
                     /translation="MNQKSLFIAALAVLLLAFGGGAMFYSMQQEQAANDLAAANRAAL
                     LRMHSPTLGPANAPVVIVEFLDPACETCAAFYPLVKQMMAANPERIRLVLRYAPFHNG
                     SDKVVAVLEAARRQGKFWPTLEALLATQADWSPHHTPQVARVWKHLEGLGLDLERMAF
                     DITAPDIAQVIAQDLADAQTLGVSKTPEFFVNGKPLPRFGYEPLKQLVDAALAAAPK"
     gene            2251..3453
                     /gene="lhgO"
                     /locus_tag="VIS73_06770"
     CDS             2251..3453
                     /gene="lhgO"
                     /locus_tag="VIS73_06770"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEY5762892.1"
                     /translation="MSQHYDFAVIGGGIVGASTAWQLQQRFPDRKVVLVEKESRPATH
                     QTGHNSGVIHAGVYYAPGSLKAQFCREGSAATLAFCDEHAIPVDRCGKLLVATDDTEF
                     ERMKALFARCEENGIAVDWIDAVGLQQLEPHIRGLGAIRVPATAIVDYRLVTARMLEL
                     FRQLGGEVRLSSEVVAIDEAAQQITVTTRAGQFTAGYLVACAGLMADRIARMAGLSID
                     FSIIPFRGEYYRLPARHNNIVSHLIYPIPDPALPFLGVHLTRMIDGSVTVGPNAVQGW
                     RREGYGGFNFSLRDSLEMLTFPGTWRLLRRHYATGLAELRDSLWKRGYLRRVRKYCRE
                     LTLADLQPYPAGIRAQAVLRDGSLVHDFLFAESPRSLHVCNAPSPAATSAMPIGAHIV
                     ERVAEAFG"
     gene            complement(3472..4260)
                     /locus_tag="VIS73_06775"
     CDS             complement(3472..4260)
                     /locus_tag="VIS73_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY5762893.1"
                     /translation="MAFEKETGGGVNALARGLELLRCFTPNSTELSNGELAERTGLPK
                     STVSRLTKTLTELGYLRHDAARRAYRVGPSVLALGYAALGNLRLRDKARPSMQEFADA
                     TGALVSLATRERLAMIYIESCRSRSSMTLRVGVGTQLPIFPTSIGRAFLAGLPASERM
                     YLVHELSQRDPGAWAVQRLGVERELARFAQHGYCLSIGEWKKDVNAAAVPVVTADSSD
                     LMVLSASGPAYAVTASRLEEDIAPRLVALARHLEAIEREGLAPD"
     gene            4395..5576
                     /locus_tag="VIS73_06780"
     CDS             4395..5576
                     /locus_tag="VIS73_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY5762894.1"
                     /translation="MSKAAFNWEDPLLLDSQLSDEERMVRDAAAAYAQDRLMPRVLES
                     FRNEHTDPKIFREMGELGLLGSTIPEQFGGAGLNYVCYGLAAREVERVDSGYRSMMSV
                     QSSLVMVPINEFGTEATKQKYLPRLATGELIGCFGLTEPNHGSDPGSMASRAKKVAGG
                     YSLSGSKMWITNSPIADVFVVWAKDDAGDIRGFVLEKGWKGLSAPAIHGKVGLRTSIT
                     GEIVMDEVFCPEENAFPTVRGLKGPFTCLNSARYGIAWGALGAAEFCWHTARQYTLDR
                     SQFGRPLAANQLIQWKLAEMQTEITLGLQGCLRLGRMKDDGTAAVEITSLMKRNSCGK
                     ALVVARMARDMLGGNGISDEFGVIRHVVNLEVVNTYEGTHDVHALILGRAQTGIPAFA
                     N"
     gene            5591..6799
                     /locus_tag="VIS73_06785"
     CDS             5591..6799
                     /locus_tag="VIS73_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY5762895.1"
                     /translation="MGALSHLRIVDLSRILAGPWATQVLGDLGAEVIKIERPAVNGQG
                     GDDTRSWGPPWWLDRDGQREAAYYLATNRNKRSVTIDFTKPEGQELVRQLVAGADVLI
                     ENFKVGGLKKYGLDYETLKAVNPRLVYCSITGFGQDGPYADRAGYDFLIQGMGGLMSI
                     TGAADGAPQKVGVALTDILTGLYAANAIQAALAHRDRTGEGQSIDIALLDVQIACLAN
                     QAMNYLVSGKAPTRLGNEHPNIVPYAAFPTADGHMILTVGNDRQFADFARLAGHPEWS
                     ADPRYATNAARVENRVELTAAINAATVARTTSEWVSILEKHAVPCGPINSLNELFIDP
                     QVLHRQMQRHLDRNGDLPQVASPIRLSATPVQYDRPPPALGRDTEAVLGDTLQLDKNK
                     LASLKDRGII"
     gene            6826..>7096
                     /locus_tag="VIS73_06790"
     CDS             6826..>7096
                     /locus_tag="VIS73_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY5762896.1"
                     /translation="MKFARTLVLSAGLLGLAATANAQQFVNVLTGGTSGVYYPLGVAL
                     TQVYGKALPNAKVTVQSTKASAENLNLLQAGKGEVAFTLGDALSDA"
BASE COUNT         1245 a         2519 c         2171 g         1161 t
ORIGIN      
        1 gctcgattgg aaccgccgcg tcttcgaagc gatccgcctg ctacccgcga cgctggtcga
       61 cacgcgcgga cgcggcatcg ccacgacgct gctcggccag cctgccgccg caccgctggc
      121 catcgcgccg accggcatca acggcatcta cgcgcgcgac gccgatctcg cgctggcccg
      181 cgccgccgcg gccgcgaaga ttccgttctg cctgagcacc gtatcgaacg cgcggctgga
      241 gacaattgcc gccgaagccg gcggccgcct gtggatgcag ctctacgtga tgaagaaccg
      301 tgacatcgcg cgcgacatca tcggccgcgc cgaggctgcc ggctacgaag cgctgatgct
      361 caccaccgac gccaacgtct tcggccatcg cgagtgggac aagcgcaact accgcgcgcc
      421 gggcgagctg agctggaaaa acctgctcga cgtcgccctg catccgcgct gggcgctcga
      481 ggtcatggca cccgacggcc tgccgccctt cgaaaacatc gtcgaccaca tgccggccga
      541 ggcgcagagt gccaaaggcg gcgtcgcctt cattccgcag cagttcgctc ccgatgttac
      601 ctgggacgaa ttcgcctgga ttcgccgtca gtggccacgc aagctgatga tcaagggcat
      661 cctcagcgcc gctgacgctc ggcgtgccgc cgatgcgggc tgcgaggcga tcgtgctctc
      721 caaccacggc ggtcgccagc tcgagagctg cgtctcgccg atcgacatgc tgcccgacgt
      781 ggtggccgcc gtcggcgatc gcctcgaggt ctacgtcgac agcggcttcc gccgcggcaa
      841 cgagatcatc aaggcgatcg cgctcggcgc caaggccgtg atggtcggcc gggccacgct
      901 ctacgggctt gctgccggtg ggcaggccgg cgtgcagcag gcgctgcaga tcctcgccag
      961 cgaaaccgac cgcacgctcg gccaactcgg ctgcaatgcg ctcgacgaac tcgggccgga
     1021 acacctgcgc cgctaagcat cgacaggtat agttcggtcc tcgcgcacgg cgcgcttttt
     1081 tccgcaccca cgccggccgg catcgccgcc taactgctgc agacctccga tgaacacaac
     1141 cgactccagt gccacgagcg cctggaacct gctcttcgcc gcctggctgg tcgccaccgg
     1201 ctcgacgctc ggcgcgctgt tcttcggcga agtgatgggg ctgccaccct gcctgctctg
     1261 ctggtggcag cgcatcttca tgtttccgct cgccgtgctg ctgccctttg gcctctttcc
     1321 ctatgactcg cggatgatcc gctatggcct ggtgctggcg attcccggcg cgctgacggc
     1381 gctgttccat cagctgctgg tgagcggcat cgtgccggag acggtcaagc cctgtaccca
     1441 gggcgttccc tgctcggaaa ccgtgatcac ctggttcggc ttcctgacga tcccgctgct
     1501 ctcgctcgcc gccttttcaa ttctcatcgc gctgctcatt gcggcgcaga tccggagcaa
     1561 ttcatgaatc agaagtcgct ttttatcgcc gcgctggcgg tcctgctgct cgcctttggc
     1621 ggcggcgcaa tgttctattc gatgcagcag gagcaagctg ccaacgacct cgccgctgcc
     1681 aaccgcgccg cgttgctgcg catgcactcg ccgacgctgg gcccggcaaa cgcgccggtg
     1741 gttatcgtcg agttcctcga ccccgcctgc gaaacctgcg cggcctttta cccgctggtc
     1801 aagcagatga tggccgccaa ccccgagcgc atccgcctgg tgctgcgcta cgcgccgttc
     1861 cacaacggtt cagacaaggt cgtcgccgtc ctcgaagcag cccggcggca aggcaagttc
     1921 tggccgacac tcgaggcgct gctggcaacg caggccgact ggtcgccgca ccacacgccg
     1981 caggtcgcgc gcgtctggaa gcacctcgaa ggcctcggcc tcgatctaga acggatggcc
     2041 ttcgacatca ccgcgcccga catcgcccaa gtgatcgcgc aggacctcgc cgacgcgcag
     2101 acgctgggtg tcagcaagac gccggagttc ttcgtcaacg gcaagcccct gccacgcttc
     2161 ggctacgaac cgctcaagca gctggtcgac gcggcgctag cggcggcacc gaagtaggca
     2221 agccatggaa cacccaacaa ggcgaccatc atgagccagc actacgactt cgccgtcatc
     2281 ggcggcggca tcgtcggcgc ctcgacggct tggcagctgc agcagcgatt ccccgaccga
     2341 aaggtggtcc tggtcgagaa ggaatcgcgg ccggcgaccc accagaccgg gcacaacagc
     2401 ggcgtcatcc acgccggcgt gtattacgcg ccaggcagcc tcaaggcgca gttctgccgc
     2461 gagggctcgg cggcgacgct ggccttctgc gacgaacacg cgattcccgt cgatcgctgc
     2521 ggcaagctcc ttgtcgccac cgacgacacc gaattcgaac gcatgaaggc gctcttcgcg
     2581 cgctgcgagg agaacggcat cgccgtcgac tggatcgacg ccgtgggtct gcagcagctc
     2641 gagccgcata tccgcggact cggcgcaatc cgcgtgccgg caactgccat cgtcgactac
     2701 cggctggtca ccgcgcgcat gctcgagctg ttccgccaac tcggcggcga ggttcgcctc
     2761 agttcggaag tcgtggcaat tgacgaagcc gcccagcaga tcaccgtcac cacccgcgcc
     2821 ggccagttca cagccggcta tctcgtcgcc tgcgccggcc tgatggccga ccgcatagcg
     2881 cgcatggcgg gcctgagcat cgatttctcg atcatcccct tccgcggcga gtactaccgc
     2941 ctgccggcgc gccacaacaa catcgtcagc catctgatct acccgatccc cgacccggcg
     3001 ctacccttcc ttggcgtgca cctgacgcgg atgatcgacg gctcggtcac cgtcggcccg
     3061 aatgcggttc agggctggcg gcgcgaaggc tatggcggat tcaatttcag cctgcgtgac
     3121 agccttgaga tgctcacctt cccgggaacc tggcggctgc tgcgccggca ctacgcaacc
     3181 ggcctggccg aattgcgcga ctcgctgtgg aagcgcggct acttgcgccg cgtgcgcaag
     3241 tattgccgcg aactgacgct ggcagaccta cagccctacc cggccggcat ccgtgcccag
     3301 gcggtgctgc gcgacggctc gctggtacac gacttcctgt tcgccgaatc gccgcgatcg
     3361 ctgcacgtct gcaacgcgcc ctcgccggcc gcgacatcgg cgatgccgat cggcgcgcat
     3421 atcgtcgagc gtgtggccga agcgttcggc tgaccggggc aaccccacaa ctcaatccgg
     3481 cgcgagccct tcgcgctcga tcgcctcaag gtgccgtgcc agcgcaacta ggcgtggcgc
     3541 gatgtcttcc tccaggcgcg aggcagtcac cgcgtaggcc gggccgctcg ccgacagcac
     3601 catcagatcg gaactgtccg ccgtcaccac cggcacggcg gcggcgttga cgtccttttt
     3661 ccactcgccg atcgacaggc agtagccatg ctgcgcgaag cgcgcaagtt cgcgctcgac
     3721 gcccaggcgc tgcactgccc aggcgccggg gtcgcgctgc gacagctcgt gcaccaggta
     3781 catccgttcc gacgccggca atccggcgag gaaggcgcga ccgatcgagg tcggaaagat
     3841 cggcaactgc gtgccaacgc cgacgcgcag cgtcatgctg ctgcggctgc ggcagctttc
     3901 gatgtagatc atcgccagcc gctctcgcgt cgccagcgac accagcgcgc cggtggcatc
     3961 ggcgaattcc tgcatcgacg gacgcgcttt gtcacgcaac cgcagattgc ctagggccgc
     4021 gtagcccagc gcgagcaccg aaggtccgac gcggtaggca cgccgcgccg cgtcgtgccg
     4081 caggtagccg agttcggtca aggtcttcgt cagccgcgat acggtggatt tgggcagccc
     4141 ggtgcgctcg gccagttcgc cgtttgacag ctccgtcgag ttcggcgtga agcagcgcaa
     4201 tagttccagc ccgcgcgcga gcgcgttgac tcctccgccg gtttcctttt caaacgccat
     4261 gccttcttca cctcatattc cgcgatacgg aacaatacta cttattatca attttctatg
     4321 gaacactttg cgatgccatg ccatatttcg cccgaccaat tccacacgcc gccgacgcgt
     4381 ttctcgagga caccatgagc aaagccgcct tcaactggga agaccccctg cttctcgatt
     4441 cgcaactctc cgacgaggag cgcatggtgc gcgacgctgc cgcggcctat gcgcaggacc
     4501 ggctaatgcc gcgcgtactc gagtccttcc gcaacgaaca taccgacccg aagatcttcc
     4561 gcgagatggg cgaactgggc ctcctcggct cgacgattcc cgaacagttc ggcggcgccg
     4621 ggctcaacta cgtctgctac ggcctcgccg cgcgcgaggt cgagcgcgtc gactccggct
     4681 accgctcgat gatgagcgtg cagagttcgc tggtgatggt gccgattaac gagttcggca
     4741 ccgaggccac caagcagaag tacctgccta ggctcgccac tggcgaactg atcggctgct
     4801 tcggcctgac cgaaccaaac cacggctccg accccggcag catggcctcg cgcgcgaaga
     4861 aggttgccgg gggctattcg ctcagcggca gcaagatgtg gatcaccaac agcccgattg
     4921 ccgacgtctt cgttgtctgg gcgaaggacg atgcaggaga catccgcggc ttcgtgctcg
     4981 agaagggctg gaagggcctg tcggcgccgg cgatccacgg caaggtcggc ctgcgcacct
     5041 cgatcaccgg cgagattgtc atggacgagg tgttttgtcc cgaagaaaac gccttcccca
     5101 ccgtgcgcgg tctcaagggg ccgttcacct gcctcaactc ggcccgttac gggatcgcct
     5161 ggggggcgct cggcgccgcc gagttctgct ggcacacggc acggcagtac acgctcgacc
     5221 ggagccagtt cggccgcccg ctcgccgcaa accagctgat ccagtggaag ctcgccgaaa
     5281 tgcagaccga aatcacgctc ggcctgcagg gctgcctgcg ccttggtcgc atgaaggacg
     5341 acggcacggc tgcagtcgaa atcacctccc tcatgaaacg caattcctgc ggcaaggcgc
     5401 tggtcgttgc ccgcatggcg cgcgacatgc tgggcggcaa cggcatttcc gacgaattcg
     5461 gcgtcatccg ccacgtcgtc aatctggaag tggtcaacac ctacgagggc acgcacgatg
     5521 tccatgcgct gatcctcggc cgtgcgcaga ccggcatccc cgccttcgcc aactgagccc
     5581 agggcagacc atgggcgcgc tctcccacct gcgcatcgtc gacctctcgc gcatcctcgc
     5641 cggcccctgg gcgacgcagg tgctcgggga tctcggcgcc gaggtcatca agatcgagcg
     5701 ccctgcagtc aatggtcagg gcggtgacga cacgcgcagc tggggcccgc cgtggtggct
     5761 cgaccgggac ggccaacgcg aggccgcgta ctacctcgcc accaaccgca acaagcgctc
     5821 ggtaacgatc gacttcacga agcccgaagg gcaggaacta gtgcgacaac tggtcgccgg
     5881 cgccgacgtg ctgatcgaga acttcaaagt cggtgggctg aaaaagtacg ggctcgatta
     5941 cgagaccctg aaggccgtca atccgcgcct cgtctactgc tcgatcaccg gtttcggcca
     6001 ggacggcccc tatgccgacc gcgccggtta cgacttcctg atccagggca tgggcggcct
     6061 gatgagcatc accggcgccg ccgatggcgc gccgcaaaag gtcggcgtcg cgctcaccga
     6121 catcctcacc ggtctctacg cggccaacgc gatccaggcg gcgctggccc accgtgaccg
     6181 gactggcgag gggcaaagca tcgacatcgc gctgctcgac gtgcagatcg cctgcctcgc
     6241 caaccaggcg atgaactacc tggtctccgg caaggcgccg acaaggctcg gcaatgagca
     6301 tccgaacatc gttccctacg cagcgttccc tactgccgac ggccatatga tccttaccgt
     6361 cggcaacgat aggcagtttg ccgacttcgc gcggctcgcc ggccaccccg aatggagcgc
     6421 cgacccgcgc tacgcgacca acgccgcccg cgtcgaaaac cgcgtcgaac tgaccgcggc
     6481 gatcaacgcc gcgaccgtcg cgcggacaac cagcgaatgg gtgtcgatcc tcgaaaagca
     6541 cgcggttccc tgcggcccga tcaactcgct gaacgaactt tttatcgacc cgcaggtctt
     6601 gcacaggcag atgcagcggc atctcgatcg caacggcgac ctgccgcagg tcgccagccc
     6661 gatccggctg tcggcaaccc cagtgcagta cgaccggccg ccaccggcgc tgggccgcga
     6721 caccgaagcc gtcctcggag atacactgca gctcgacaag aacaaacttg cttcactaaa
     6781 agaccgcgga atcatctgac cgcgaaaaac caaaggagac gttccatgaa gttcgcccgt
     6841 accctcgtgc tgtctgccgg cctgctcggc ctggctgcca cggccaatgc ccagcaattc
     6901 gtcaatgtcc tcaccggcgg caccagcggc gtctactacc cgctcggagt ggcgctgacg
     6961 caggtctatg gcaaggcatt gccgaacgcc aaggtcaccg tgcagtcgac caaggcctcg
     7021 gcggaaaacc tcaacctgct gcaggcgggc aagggcgaag tcgccttcac gctcggcgac
     7081 gcgctgtccg acgcat
//
LOCUS       DATDAA010000213         3131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_470482,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000213 DATDAA010000000
VERSION     DATDAA010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470482"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="VIS73_06795"
     CDS             <1..1331
                     /locus_tag="VIS73_06795"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY5762897.1"
                     /translation="APFVEIEWGREATAIMRELKALLDPDGLLNPGVVLNEDPTVHLK
                     NLKPMPAADAIVDACIECGFCETQCPSAALTFSPRQRIVATRDFARRAANPAEAPLSG
                     AVETYRYMGMDTCAGCGLCSTVCPVGINTGQLIRSQRGRDASGTTRFIGRTAARHFST
                     TATFARLGVAAGRAASAVVGDSALSRITGGRWKAGLRPGTTPPVCSMGGDGARRVVYF
                     AGCAGRMMGDSGKPLSAAVIDTCQRAGFEVIQPKELGGLCCGQPWESKGLFDDARDKA
                     NELEAALAEASERGRWPIVFDASACALRMREHIAGRLPIVDFPVFAADQLLPRLAIRR
                     QKPAIALHLNCSSRRMGDQNKVMACASACAEKVVVPPDVKCCGFGGDRGFAVPELNAH
                     ALRTLTPDLLKGCEAGYSSNRTCEIGLTDRTGIPYRSLAHLLDECTEERS"
     gene            1372..2211
                     /locus_tag="VIS73_06800"
     CDS             1372..2211
                     /locus_tag="VIS73_06800"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEY5762898.1"
                     /translation="MTLERTVYFVSDGTGITAENLAHSLLSQFEDVRFRQIRIPFVDC
                     AEKASAVVERINESARREGSRPIVFTTLIDNGLSAIVHSADAFCLSFFETFLAPLEAE
                     LAAKSTHTVGRLHGSADSFGYKNRIEAINYTLLHDDGITNHGLEEADVILVGVSRCGK
                     TPTSLYLAMQFGVKAANFPLIPEDFERNALPGSIERFREKLFGLTIRPERLNQIRSER
                     RPGSNYASLDNCRYEIREAEKLMRRESIRWLDSSAKSIEEISATVMQTLRGENESFSR
                     FGA"
     gene            2453..>3131
                     /locus_tag="VIS73_06805"
     CDS             2453..>3131
                     /locus_tag="VIS73_06805"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY5762899.1"
                     /translation="MTTDRADGLRGIGVCLLLGMLACSAHAGGSTKFPDATASDPAAL
                     GWMVGSPPPAEKTLRFDDGSYFRFPAMRWSVANFRQLMPTVNVSRGLGESSALEQRLR
                     PEIDAIRFVPLGGGEPMTWAQSLLANYTDGIIVLHRGSVVFERYFGVLKPEGQHGAMS
                     VTKTFVGTLAALLASEGAFDPTRTVAHYVPELSASAFGNATVRQLMDMTTGIRFSENY
                     ADPKAEVW"
BASE COUNT          551 a         1028 c          969 g          583 t
ORIGIN      
        1 tggcaccctt cgtcgaaatc gaatggggcc gcgaggcgac ggcgattatg cgcgagctga
       61 aggccctgct cgaccccgat ggcctgctca accccggcgt cgtgctcaac gaagacccga
      121 ccgtacacct caagaacctc aagccgatgc cggctgctga cgcgatcgtc gacgcctgca
      181 tcgagtgtgg cttctgcgaa acgcagtgcc cgtcggccgc cctcaccttt tcgccccgcc
      241 agcgcatcgt cgccacccgc gacttcgcac ggcgggcagc gaatccggcc gaggcgccgt
      301 tgagcggcgc ggtcgaaacc taccgctaca tgggcatgga cacctgtgct ggctgcggcc
      361 tctgctcaac ggtctgcccg gtcggcatca acaccggtca gctgatccgc agccagcgcg
      421 gacgcgatgc gagcggcacg acacgcttca tcggccgtac tgcggcccgc catttctcga
      481 cgaccgcaac tttcgcccgc ctcggtgtcg ctgccggtcg tgccgccagc gccgtcgttg
      541 gcgattcggc gttgagccga attactggcg gccgctggaa ggccggattg cgtcctggca
      601 caacgccgcc ggtctgcagc atgggcggcg acggtgcgcg gcgcgtcgtc tatttcgccg
      661 gctgcgccgg tcgcatgatg ggtgattccg gcaagccgct gtcggctgct gtcatcgata
      721 cctgtcagcg cgccggcttc gaggtaatcc agcccaagga actgggcggc ctatgctgcg
      781 gccagccatg ggagagcaag gggctgttcg acgatgcccg cgacaaagcc aacgaactcg
      841 aagcggcgct ggcagaggcc agcgaacgtg gccgctggcc tattgtcttc gacgccagcg
      901 cctgcgcgct gcgcatgcgc gaacacattg cgggccgcct tccgatcgtt gacttcccgg
      961 tcttcgccgc cgaccagttg ctgccgcggc tggcgatccg ccgccagaag ccggcgatcg
     1021 cactgcacct caactgctcg agccggcgaa tgggcgacca gaacaaggtg atggcctgcg
     1081 ccagtgcctg cgccgagaag gtcgtcgtcc cgcccgacgt gaaatgctgt ggctttggcg
     1141 gcgatcgcgg cttcgccgta cccgaactca acgcccacgc gctgcgcacg ctcacgcccg
     1201 atctgctcaa gggctgcgag gccggctact cgtcgaaccg gacctgcgag atcggtttga
     1261 ccgaccgcac cggcattccg tatcgttcgc ttgcgcatct gctggacgaa tgcacggagg
     1321 agcggagctg acgactccgc gtttccagca cactggggag cacacggggt catgacgctg
     1381 gaacggacgg tctatttcgt ctccgacggg acggggatca ccgcggaaaa cctcgcgcac
     1441 agcctgctct cgcagttcga ggacgtgcgc ttccggcaga tccggattcc cttcgtcgat
     1501 tgcgcggaga aggctagcgc cgtagtcgag cgaatcaacg aatcggcacg ccgcgagggc
     1561 agccgcccca tcgtcttcac gacgctgatc gacaacggac tgtcggcgat cgtgcactcg
     1621 gccgacgcct tctgcctgtc gtttttcgag actttcctcg cgccgctcga agccgagctg
     1681 gcggccaagt cgacgcacac tgtcggccgc ctgcacggtt ctgccgacag cttcggctac
     1741 aagaaccgca tcgaggcgat caactacacg ctcttgcacg acgacgggat caccaatcac
     1801 ggactcgaag aagccgacgt catcctcgtc ggcgtctcgc gctgcggcaa gacgccgacg
     1861 agcctctatc tcgccatgca attcggcgtc aaggcggcta acttcccgct gatacctgaa
     1921 gacttcgagc gcaacgccct gcccggcagc atcgaacgct tccgtgaaaa gctgttcggc
     1981 ctgaccatcc gccccgagcg tctaaaccag attcgcagcg aacgccggcc cggcagcaac
     2041 tacgcgtcgc tcgacaactg ccgctacgaa atccgcgagg ccgagaaact catgcgccgc
     2101 gagtcaattc gctggctcga ctcctctgca aagtcgatcg aggagatctc ggcaacggtc
     2161 atgcagacgc tgcgcggtga aaatgaatcg ttctcacgat ttggtgcctg acccctggtg
     2221 catgggcatc tcagcctgat cggcgcgaac accggcatct atgccattcg tttgcgcatt
     2281 tagaccgatt ggagcgcttc ggcgcagctg cgagccgaat tccaatatcg gcgtcgcttc
     2341 ttcggcgcct gttcccggga caacttttcg gctcatgccg ggtcggactt agcgatccag
     2401 actcgaatag ctttcggctt gtgcgttctg gaatatcgac taggagggtt gcgtgacgac
     2461 agatcgggcg gatggactac gcggtattgg cgtttgcctg ttgcttggca tgcttgcctg
     2521 ctcggcgcat gccggcgggt cgacgaagtt ccccgacgca accgcgagtg acccagcagc
     2581 actggggtgg atggtcggct cgccgccgcc ggcggaaaag actctgcgct tcgacgacgg
     2641 cagttacttt cgttttccgg cgatgcgctg gagcgtcgcg aacttccgac aactcatgcc
     2701 aaccgtcaat gtatcgcgcg ggctgggcga atcctccgcg cttgaacaga ggctaaggcc
     2761 ggaaatcgac gcgattagat ttgtgcctct cggcggcggt gagccgatga cttgggcgca
     2821 atctcttctg gccaactaca cggacgggat catcgtcctc catcgtggtt cagtcgtttt
     2881 cgagcgctat ttcggcgtct tgaagcccga aggacagcat ggtgcgatgt cggtcaccaa
     2941 gacattcgtt ggcacgctgg cggcgttgct ggcgtcagaa ggggcgtttg atcccacgcg
     3001 gactgtcgcc cactacgttc ccgagttatc cgcgtcagca ttcgggaatg ctacggttcg
     3061 ccagctcatg gacatgacca ccggcattcg cttcagcgag aattacgctg atccgaaggc
     3121 cgaggtctgg g
//
LOCUS       DATDAA010000214         4914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_155538,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000214 DATDAA010000000
VERSION     DATDAA010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155538"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2132)
                     /locus_tag="VIS73_06810"
     CDS             complement(<1..2132)
                     /locus_tag="VIS73_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HEY5762900.1"
                     /translation="MSQITRSTCPYCGTGCGVLIQHDGARITGVEGDPEHPANFGRLC
                     TKGSTLHLTTLATGRALYPMRFSRSAEVGGEQIATRITWDAALDEAAGRFAEIIEQHG
                     PDAVAFYVSGQLLTEDYFVFNKLARVLVGTNNIDSNSRLCMSSAVSAYKRTLGADAPP
                     CNYEDIDTADVVLVAGANPAWAHPILFRRLSDARAKDRERKLIVVDPRRTDTAAAADL
                     HLQIEPGTDTILFGAMLHVLLWDGLTNGAFVREHTSGFDAAREAVRELTPAVAATRCG
                     VKAEDIVRAARWFGNAGSALSLWCQGLNQSHHGSDNGAALIHLHLATGQIGRAGAGPF
                     SLTGQPNAMGGRETGTMANLLPGHRDPADADDRAELAALWGVPELPATPGRTATEIID
                     GLHDGSIKAVWIACTNPAQSLPDQARVREAFGRADFVVLQEAYADTETAAFADLLLPA
                     ATWGEKEGTVTNSERRITRVQAAVSVPGEARPDWAIARSFAQVLARRLGLDDLLNHFG
                     WETTAAIFDEHAALTAGRDLEFSALDYAALERDGPQQWPYRAGQGTDRLYGDRRFATP
                     DGRARFVPVSLVATAEATSARYPIHLNTGRLRDQWHGMSRTGKLAYLYNHSERCTIVL
                     APGDMARRGLVEGELLRVRSGRGEVILPLAADASLKSGHAAIPMHWGRRRLSHSAANE
                     LLAAVVDPLSKQPELKHAAIRIESAALA"
     gene            complement(2153..2464)
                     /gene="nirD"
                     /locus_tag="VIS73_06815"
     CDS             complement(2153..2464)
                     /gene="nirD"
                     /locus_tag="VIS73_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HEY5762901.1"
                     /translation="MHYHDICAIDEIPRLGSRVVAAPAGDIAVFRTADDRVFALNDQC
                     PHKGGPLSQGIVHGDRVACPLHGWLIQLESGAAVAPDEGCVKRHEAKVENGRVLLRVA
                     G"
     gene            complement(2476..>4914)
                     /gene="nirB"
                     /locus_tag="VIS73_06820"
     CDS             complement(2476..>4914)
                     /gene="nirB"
                     /locus_tag="VIS73_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HEY5762902.1"
                     /translation="MAGCRVLEELMKLAPDLYEITVFGAEPHGNYNRILLSPVLAGEM
                     TLKDIVLNDLDWYAGHGITLHAGRTVTRIDRVARKVIADDGQGGIIEVGYDRLLIATG
                     STPFIPPIPGNDLDGVLGYRDIADTEKMIAAAGEYRHAVVIGAGLLGLEAANGLKLRG
                     MDVTVIHLGEWIMERQLDQTAAGLLQSALEARGLKFMLSAQTAALQEKGGRVGSVKLK
                     DGTEIAADLVVVAAGIRPNTALAAAAGLHINRGIVVSDTLQTVTDPRIYAVGECASHR
                     GIAYGLVAPLFEQARVCATHLAEFGIGRYQGSLTSTKLKVTGIDVFSAGDFAGGKHTE
                     EIVLHDKSGGVYKKLVLKGDQLVGACLIGDTADGAWYFRLLKDGTDVRELRDHLMFGE
                     ASLGDAGHAGGHTAANLPDTAEVCGCNGVCKGTIVRAIRENGLFTLDEVKKHTKASSS
                     CGSCSGLVEQILASTVGGAYKPVSLADKPVCACTDHPHGTVRKAIVEHKYLSIPAAMQ
                     GLGWRTPNGCATCRPALNYYLISSWPHEAEDDPQSRFVNERAHANIQKDGTYSVVPRM
                     WGGLTTPDELRAIAHVAEKYQVPTVKVTGGQRIDLLGVKKEDLPKMWADLGKAGMVSG
                     HAYGKSIRTVKTCVGAEHCRFGTQRSMEMGVQLEKMLFAMWSPHKVKLAVSGCPRNCA
                     EAGIKDVGIIGVDSGWELHIAGNGGIKTEVAQFLCKVETAEEVKEISGAFLQLYREEG
                     FYLERTVHYVTRVGFDHVKSRVVEDVENRKALYERLLYSLQGLPDPWAEQVADESKRR
                     AFIPIKLAADAVPA"
BASE COUNT          803 a         1675 c         1598 g          838 t
ORIGIN      
        1 caggcgagcg ccgcggactc gatgcggatc gccgcgtgct tgagttcggg ctgcttcgac
       61 agcgggtcga cgacggcggc gagcagttcg ttggcggccg agtggctcag tcgccgccgc
      121 ccccagtgca tcggaatcgc cgcatgtcca gacttcagcg acgcgtcagc cgcgagcggc
      181 aggatcacct cgccgcgacc actccgcacg cgcagcagtt cgccctcgac gagcccgcgc
      241 cgcgccatgt cgccgggagc cagcacaatc gtgcagcgct cgctgtggtt gtacagatac
      301 gcgagctttc cggtgcggct catgccgtgc cactggtcgc gcaggcgccc ggtgttcagg
      361 tgtattgggt agcgcgcgct ggtggcttca gcagtggcaa cgagactgac cggaacgaag
      421 cgcgcacgcc cgtccggcgt ggcgaaacgc cggtcaccgt agagacggtc cgtgccctgc
      481 ccggcgcgat atggccactg ctgcgggccg tcgcgctcga gcgccgcgta atcgagcgcc
      541 gagaactcga ggtcgcgccc ggcggtcagc gccgcgtgtt cgtcgaagat cgcggcggtc
      601 gtctcccagc caaagtgatt cagcaaatcg tctagcccga gccgacgcgc gagcacctgc
      661 gcgaacgacc gggcgatcgc ccagtccggc cgcgcctcgc cgggcaccga aacggccgcc
      721 tgcacccgcg tgatgcgccg ctccgaattg gtgaccgtgc cctccttctc gccccaggtt
      781 gcggccggca gcagcagatc ggcgaaggcc gcggtttcgg tatcggcgta ggcctcctgc
      841 agcacgacga aatcggcgcg cccgaatgcc tcgcgcacgc gcgcctggtc gggcagcgac
      901 tgcgcggggt tggtgcaggc aatccatact gccttgatcg agccatcgtg gaggccgtcg
      961 atgatctcgg tcgcggtccg gcccggcgtt gctggcaatt cgggaacgcc ccagagcgcc
     1021 gccagttcgg cacgatcgtc ggcgtccgcc ggatcgcgat ggccgggcag caggttggcc
     1081 atcgttccgg tttcccgccc gcccatcgcg ttgggctgcc cggtcagcga gaacggcccg
     1141 gctccagcgc ggccgatctg gccagtggcc agatgcaggt gaatcagcgc cgcgccatta
     1201 tccgatccgt ggtgcgactg gttgagcccc tggcaccaca gcgacagcgc cgacccagcg
     1261 tttccgaacc agcgcgccgc gcggacgatg tcctctgcct tgaccccgca gcgcgtcgcg
     1321 gcgaccgccg gcgtcagttc gcgaacggct tcgcgcgcgg catcgaagcc gctggtgtgc
     1381 tcgcggacga aggcgccgtt ggtcagtccg tcccaaagca gcacatggag catcgcgccg
     1441 aagaggatgg tgtcggtgcc cggttctatc tgcaggtgaa gatcggctgc cgccgccgtg
     1501 tcggtacgtc gcgggtcgac gacgatcagc tttcgctccc gatccttcgc gcgcgcgtcg
     1561 ctcagccgcc gaaacaggat cggatgcgcc caggccggat tggcgccggc gaccagcacg
     1621 acatcggccg tatcgatgtc ctcgtaattg cagggcggcg catcggcgcc cagcgtgcgc
     1681 ttgtaggccg acacggcgct gctcatgcag aggcgcgaat tcgaatcaat gttgttggtg
     1741 ccgaccagca cgcgggcgag cttattgaag acaaagtagt cctcggtcag cagttgcccc
     1801 gacacgtaga aggccaccgc atcgggtccg tgctgctcga tgatctcggc aaagcgaccg
     1861 gcggcttcgt cgagcgccgc atcccaagtg atccgcgtcg caatttgctc gccgccaacc
     1921 tcggccgacc ggctgaagcg catcggatag agcgcccgac cggtcgccag cgtcgtcaga
     1981 tgtaacgtcg accccttggt gcacagacgg ccgaagttgg ccggatgctc cggatcgcct
     2041 tcgacacccg tgatccgcgc accatcgtgc tggatcagca cgccacaccc tgtaccgcaa
     2101 taggggcagg tggaacgggt aatttggctc atgacggcgc ccggaaatcc gctcagccag
     2161 caacgcgcag cagcacgcgc ccgttctcga ccttcgcttc gtgtcgcttg acgcagcctt
     2221 cgtcgggtgc caccgccgcg cccgattcga gctggatcag ccagccgtgc agcggacagg
     2281 caacgcggtc gccgtggacg atcccctgcg agagcggccc acccttgtgc gggcattggt
     2341 cattcagtgc gaaaacgcga tcatcggcag tacgaaagac cgcgatgtcg cccgccggcg
     2401 cggcgacgac gcgcgagccg agccgcggaa tctcgtcgat ggcgcagatg tcgtggtagt
     2461 gcatggtcgt tctccttagg cgggaaccgc gtcagcggcg agcttgatcg ggatgaaggc
     2521 gcggcgtttg ctctcgtcgg ccacctgctc cgcccacggg tccggcaacc cctgcagcga
     2581 gtacaacaac cgctcgtaga gcgccttgcg gttctcgacg tcctcgacga cgcgtgactt
     2641 gacgtggtcg aagccgacgc gcgtcacgta atgcacggtc cgctcgaggt agaagccctc
     2701 ctcgcgatag agctgcagga aggcgccgga gatttccttc acttcttcag cggtctcgac
     2761 cttgcacagg aactgcgcga cctcggtctt gattccgcca ttgccggcaa tgtgcagttc
     2821 ccagccggaa tcgacgccga tgattccgac gtccttgatg ccagcctcgg cgcagttacg
     2881 cgggcaaccg gatacggcca gcttgacctt gtgcggactc cacatggcga acaacatctt
     2941 ctcaagctgc acgcccatct ccattgagcg ctgcgtgcca aagcggcaat gctccgcgcc
     3001 gacacaagtc ttcaccgtgc ggatcgactt gccgtaggcg tggcccgaga ccatgccggc
     3061 cttgccgagg tcggcccaca tcttcggcag gtcttccttc ttcacgccaa gcaggtcgat
     3121 gcgctggccg ccggtgacct tgaccgtcgg cacctggtac ttctcggcaa cgtgggcgat
     3181 ggcgcgcagt tcgtcaggcg tggtcagccc gccccacatg cgcggcacca ccgagtaggt
     3241 accgtccttc tggatgttgg cgtgggcgcg ttcgttgacg aaccggctct gggggtcgtc
     3301 ctcggcctcg tgcggccaac tcgagatcag gtagtagttg agcgcgggcc ggcaggtcgc
     3361 gcagccgttt ggcgtgcgcc agcccaaccc ctgcatcgcc gccgggatag acaggtactt
     3421 gtgctcgacg atcgccttgc gcacggttcc gtgcgggtga tcggtacagg cgcacaccgg
     3481 cttgtcggcc aggctcactg gcttgtaggc gccgcctacc gtcgacgcga ggatttgctc
     3541 gaccagcccc gagcaggagc cgcaggacga cgatgccttg gtgtgcttct tgacctcgtc
     3601 gagcgtgaac agcccgttct cgcggatcgc cctgacaatg gtgcccttgc acacgccatt
     3661 gcatccgcaa acttccgccg tatccggcag gttcgcagcg gtgtgcccgc cggcgtgtcc
     3721 ggcgtcgccc agcgatgcct cgccgaacat caggtgatcg cgcagctcgc gcacgtcggt
     3781 gccgtccttc aacaggcgga agtaccaggc gccgtcggcg gtgtcgccga tcaggcaggc
     3841 gccgaccagc tggtcgccct tcagcacgag cttcttgtaa accccgcccg acttgtcgtg
     3901 cagcacgatc tcctcggtgt gcttgccgcc agcgaaatcg ccggcagaga agacatcgat
     3961 gccggtgacc ttgagcttgg tcgaggtcag cgagccttgg tagcgaccga ttccgaactc
     4021 cgcgagatgc gtcgcacaga cgcgtgcctg ctcgaacagc ggcgcgacga ggccataagc
     4081 aatgccgcga tgcgacgcgc attccccgac cgcgtaaatg cgcgggtcgg tcaccgtctg
     4141 cagcgtgtcg gaaacgacaa tgccacggtt gatatgaagg ccggccgcgg ccgccagcgc
     4201 cgtgttcggt ctaataccgg cagcgacgac gacgagatcg gcggcgatct cggtgccgtc
     4261 cttgagcttc accgagccga cgcgcccgcc cttctcctgc agcgccgccg tctgcgcgct
     4321 gagcatgaac ttcaggccgc gcgcctcgag cgccgactgc agcaacccgg cggcggtttg
     4381 gtcaagctgc cgctccatga tccactcgcc cagatggata accgtgacat ccatgccgcg
     4441 cagcttcagc ccgttcgccg cctcaagacc gagcaggccg gcaccgataa ccacggcgtg
     4501 ccggtactcg ccggcggcgg cgatcatctt ctcggtgtcg gcgatgtcgc gatagccgag
     4561 aacgccgtcg aggtcgttgc cgggaatcgg cggaatgaac ggcgtcgaac ccgtggcgat
     4621 cagcaggcgg tcgtagccca cctcgatgat tccaccctgc ccgtcgtcgg cgattacctt
     4681 gcgcgcaacg cggtcgatcc tggtgaccgt tctgcccgcg tgcagggtga tgccatggcc
     4741 ggcgtaccag tcgagatcgt tgagaacgat gtccttgagc gtcatctccc ccgccaatac
     4801 cggcgagagc aggatacggt tgtagttgcc gtggggttca gcaccgaaga ccgtgatctc
     4861 gtacaaatcg ggcgcgagct tcatcaactc ttcgagcaca cggcagccgg ccat
//
LOCUS       DATDAA010000215         3541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_470629,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000215 DATDAA010000000
VERSION     DATDAA010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470629"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1940)
                     /locus_tag="VIS73_06825"
     CDS             complement(216..1940)
                     /locus_tag="VIS73_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5762903.1"
                     /translation="MQSRRLCRAARLRLAIALFALPGLAGAQGATSTPATPRLVLQIT
                     VDALRGDLPRRYAHVLGEDGFRYLWNHGVQYSNAHYGHANTETIVGHVSLATGTVPAA
                     HGMVGNVWFDRAQGRLTYNIEDARYRLLTAGADVDKKTEIDPTQKAARADGRSPAAIL
                     SSTFSDELAVHFNGRSKIFAVSVKDRGAVSMAGHAGKAFWFSKSSGEFVTSTYYYKKY
                     PDWVSAWNARQPALAYAGTSWTLLHPRSKYLFGDADERAYETDFPGFGRTFPHAYGEA
                     GDKYLTTRLTLGPAGDALTLDFAKTLLDNERLGQDDVPDYLAVSFSSTDYVGHLFGAS
                     SLESEDNLAHLDRRLADLLAYVDEKVGLENTLIVLSADHGQPEVPGYLHDLGIEGGRY
                     LDPRSLDRAPAIAALKKQFGLGEELIEAFFQPYVYLNRKLIQDKGLDPAEVEKAVANE
                     LQKFPGVAAAISSTELRTAGLPDTVLTRAILRNFHPKRSGDIYLVFEPNVFINDFDGL
                     TVASTHGSPWRYDSFVPLIFAGGGLRPRTVSRRVTPYDVAPTLSAYLGVKPPSASIGD
                     PLPEVLGK"
     gene            complement(2032..>3541)
                     /locus_tag="VIS73_06830"
     CDS             complement(2032..>3541)
                     /locus_tag="VIS73_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEY5762904.1"
                     /translation="ASLAFPAAAQDKKPNILVIWGDDIGTWNISHNNRGMMGYRTPNI
                     DRIAKEGVAFTDYYAQQSCTAGRAAFIGGTVPVRTGMTKVGLPGAKEGWQKTDVTIAT
                     VMKSQGYATGQFGKNHQGDRDEHLPTMHGWDEFLGNLYHLNAEEEPENEDYPKNPEFK
                     KKFGPRGVIHSWALPDGKQKIEDTGPLTKKRMETIDDETSERAIAFIREQQKAGKPWF
                     VWWNGTRMHFRTHVKKERRGISGQDEYSDGMVEHDMHVGKFLKLLDELKIADNTIVFY
                     STDNGPHYNTWPDAGTTPFRSEKNSNWEGAYRVPAFIRWPGKFPADTTLNGIVAHEDW
                     LPTFAAAAGDAGVKEKLKKGASLNGRNYKNYIDGHNQLDYLAGKAKASPRKEFMYVND
                     DGQIVAIRYTDWKVVFLENRGQAFGVWREPFTELRVPLLFHLRRDPFERAQHNSNTYN
                     DWFLDRPFVIVPLQGFAARFLQTMQEFPPSQSPGSFNLTKIEEQLRKNAGAR"
BASE COUNT          573 a         1130 c         1154 g          684 t
ORIGIN      
        1 gcggccgccg gaaaaggtgt caggaacctt ttgcagcgtg cacttgtttc cgccgcggcc
       61 tgcagaatct ataaagtttg tgcgtttccc ggcgacgccg ggaatgcggc gacgaaacag
      121 ggtctgcagc catcttggcg ccctcgagcc ggcgctcctc gcgtcgggcg tgaagccctc
      181 cccctggcct gcgcgggcgc gcgcgccaag cccgcttact tgccgagcac ttcgggcagc
      241 gggtcgccga tcgaggccga cggtggcttg acaccgaggt aggcggacag cgtcggcgcg
      301 acgtcatacg gcgtgacccg gcgactgacc gtccgcggcc tcaggccacc gcccgcgaat
      361 atcagcggga caaagctgtc gtatcgccac ggcgagccat gggtcgaggc gaccgtcaag
      421 ccatcgaagt cattgatgaa cacgttcggc tcgaacacca gatagatgtc gccggagcgc
      481 ttcggatgaa agttgcgcag gatcgcgcgc gtcagcacgg tgtcgggcag cccggcggtg
      541 cgcagttccg tgctggagat cgcggctgcg acgccgggaa atttttgcag ctcgttggca
      601 acggcctttt ccacctcggc cgggtccaag cccttgtcct ggatcagctt gcgattgaga
      661 tagacatacg gctggaaaaa ggcttcgatc agttcctcgc caagcccgaa ttgctttttc
      721 aatgccgcga tcgccggcgc ccggtccaat gaccgggggt cgaggtagcg accgccttcg
      781 atgcccaagt catgcaaata ccccggcact tcgggctgac cgtgatcggc agagagcacg
      841 atcagtgtgt tctccagtcc gactttctcg tcaacgtagg ccagcagatc cgccagcctg
      901 cggtcgagat gcgccaggtt gtcttcgctc tccaggctgg aggccccgaa caggtgcccg
      961 acgtaatccg tcgaggaaaa gctgacggcg aggtaatcgg gtacgtcatc ctgtccgagc
     1021 cgttcgttgt ccagcagcgt cttggcgaaa tcgagcgtca acgcgtcgcc ggccggtccg
     1081 agcgtcaggc gcgtggtcag gtacttatca cccgcctcac cgtaggcatg ggggaaggtg
     1141 cgcccgaaac ccggaaagtc ggtttcgtag gcgcgctcgt cggcgtcgcc gaacaggtac
     1201 ttcgaccggg ggtgcagcag ggtccacgac gtgcccgcgt atgccaaggc gggctggcgg
     1261 gcgttccagg cgctcaccca gtccggatac ttcttgtagt agtacgtact ggtgacgaat
     1321 tcgccgctgg atttggagaa ccagaaggcc ttgccggcat ggccggccat cgaaaccgcg
     1381 ccgcgatcct tcaccgacac ggcgaagatc ttcgaccggc cgttgaaatg cacggccagc
     1441 tcatcgctga aggtcgagga caggatggcg gccggcgaac ggccatcggc cctggccgcc
     1501 ttctgcgtcg gatcaatctc ggttttcttg tccacgtccg cgccggccgt gagcaagcgg
     1561 tagcgcgcgt cctcgatgtt gtaggtcaag cggccctgcg cgcgatcgaa ccagacgttg
     1621 ccgaccatgc catgcgccgc cggcacggtc ccggtggcca gcgacacatg cccgacgatg
     1681 gtctccgtat tggcgtgccc gtagtgcgcg ttgctgtatt gcacgccgtg gttccaaagg
     1741 tatcgaaagc cgtcctcgcc cagcacatgc gcatagcggc gcggcaggtc cccgcgcagc
     1801 gcatcaaccg taatctgcaa taccagacgt ggcgtggcgg gcgtcgacgt cgcgccctgt
     1861 gcgccggcca gacctggaag cgcgaaaagg gcgatcgcca gcctaagccg cgccgcgcgg
     1921 cagaggcgcc gtgattgcat gtccttgttg ggctccggtt tcagcctaaa acgaaacggg
     1981 ccgtcatccg cttccggacg atggcccgcg tcgaaaaccg agcgcagaga atcagcgcgc
     2041 cccggcgttc ttgcgcagct gctcctcgat cttcgtcagg ttgaacgagc ccggcgactg
     2101 gctcggcggg aactcctgca tcgtctgcag gaaccgcgcg gcgaacccct gcagcggcac
     2161 gatgacgaac ggccgatcga ggaaccagtc gttgtaggtg ttggagttgt gctgcgcccg
     2221 ctcaaacggg tcgcgacgca ggtggaacag cagcggcacg cgcagctcgg tgaacggctc
     2281 gcgccagacg ccaaaggcct ggccccggtt ctcgaggaac acgaccttcc agtccgtgta
     2341 gcggatggcg acgatctggc cgtcgtcgtt tacgtacatg aactccttgc gcggcgacgc
     2401 ctttgccttg cccgcgagat agtcgagctg gttgtggccg tcgatgtagt tcttgtagtt
     2461 gcggccgttg agcgaggcgc ccttcttcaa tttctccttg accccggcgt cgcccgccgc
     2521 ggcggcgaag gtcggcagcc agtcctcgtg cgcgacgatt ccgttgagcg tcgtatcggc
     2581 gggaaacttc ccgggccagc gaatgaacgc gggcacgcga tatgcgccct cccagttcga
     2641 gttcttctcg ctgcggaacg gcgtcgtgcc ggcgtccggc caggtgttgt agtgcgggcc
     2701 gttgtcggtg gagtagaaaa cgatcgtgtt gtcggcgatc ttcagctcgt ccagcagctt
     2761 gaggaacttg cccacatgca tgtcgtgttc gaccatgccg tcggagtact cgtcctgacc
     2821 ggagatcccg cggcgctctt tcttcacgtg cgtgcggaag tgcatgcgcg tgccgttcca
     2881 ccacacgaac cagggcttgc ccgctttctg ctgctcgcgg atgaacgcga tcgcgcgctc
     2941 ggacgtctcg tcgtcgatcg tctccatgcg cttcttggtg agcggaccgg tgtcctcgat
     3001 cttctgcttg ccgtccggca gcgcccagga gtggattacg ccgcgcgggc cgaacttctt
     3061 cttgaactcc gggttcttcg ggtaatcctc gttctccggc tcctcttccg cgttcaggtg
     3121 gtagagattg cccaggaact cgtcccagcc gtgcatcgtc ggcaggtgtt cgtcgcggtc
     3181 gccctgatgg ttcttgccga actggccggt cgcataaccc tggcttttca tcaccgtcgc
     3241 gatcgtgacg tcggtcttct gccaaccctc cttcgcgccc ggcaggccga ccttggtcat
     3301 gccggtgcgc acaggcacgg tgccgccgat gaacgcggcg cgcccggccg tgcaggactg
     3361 ctgcgcgtag tagtcggtga aggcgacgcc ctctttcgcg atgcggtcga tgttgggcgt
     3421 gcgatagccc atcatgccgc ggttgttgtg gctgatgttc caggtgccga tgtcgtcgcc
     3481 ccagatgacg agaatgttcg gcttcttgtc ctgcgcggcg gccggaaacg cgaggctcgc
     3541 g
//
LOCUS       DATDAA010000216         2226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_226072,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000216 DATDAA010000000
VERSION     DATDAA010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226072"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..367)
                     /locus_tag="VIS73_06835"
     CDS             complement(44..367)
                     /locus_tag="VIS73_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEY5762905.1"
                     /translation="MSSDFWLWFTLLCIAAATHLPRASFIVLGGRVALPPALQRALRY
                     APAAALAAIIAPDVLLVQGEFALLNPKIVATAVVIACVAISRNPWLPFIAGMLSLIGL
                     RWLLA"
     gene            complement(364..1068)
                     /locus_tag="VIS73_06840"
     CDS             complement(364..1068)
                     /locus_tag="VIS73_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEY5762906.1"
                     /translation="MSESFRAGSREGFMVFLPLSIGLVPWAMVTGIAMIGAGLTPVQA
                     MGMNIIVFAGTAQIGTLPLIAIGAPMWLIVVTALVLNLRFVIFSAAISHGFRGVSTPL
                     RWLSGYLLTDGVFAVCLNKMLDVEDRAWRLGYYLAPSIFTWILWQVFALIGVLAADSI
                     PKDWSLEFMASIALMVLLAPMVKARPMLVAATTGGVVAVLLSGLPLRMGVIVAIVAGI
                     AAGFAAEHWATPEGKI"
     gene            1189..1533
                     /locus_tag="VIS73_06845"
     CDS             1189..1533
                     /locus_tag="VIS73_06845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY5762907.1"
                     /translation="MSTTTTASGLIIEELEVGSGETAAAGMHVQVHYTGWLTDGTKFD
                     SSKDRDEPFDFPLGGRRVIAGWDEGVAGMQIGGKRKLTIPPQLGYGARGAGGVIPPNA
                     TLIFEVELLAVE"
     gene            complement(1546..>2226)
                     /locus_tag="VIS73_06850"
     CDS             complement(1546..>2226)
                     /locus_tag="VIS73_06850"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5762908.1"
                     /translation="GRAATAEEIESLSWFVRIYANYVGLLDYSEFDTLTGLLNRKTFD
                     DAFERLLAKTQDEHSASSVERRRDSPSDWWMAVVDIDFFKRVNDNFGHLFGDEVLLRV
                     ANLMRESFRGYDLLFRFGGEEFVVMLHTATEEGAKVALDRFRQAVEKHVFPQVGQVTC
                     SIGYTVVDPMSAQTDLLGQADAALYWCKENGRNRIGGYGRLVSGGQLKSTTVEAVQPD
                     IDIDALFG"
BASE COUNT          442 a          701 c          748 g          335 t
ORIGIN      
        1 ggcatcgagc gccgccgccg tggcgaagta accggaagcc gagctaggcc agcagccagc
       61 gcaggccgat caggctgagc atgccggcga tgaagggcag ccacggattg cgcgagatcg
      121 cgacgcaggc gatgaccacc gcagtggcga cgatcttcgg gttgagcagg gcaaactcgc
      181 cctgcacgag aagcacgtct ggggcgatga tcgctgccag cgcagctgcc ggcgcgtagc
      241 gcagcgctcg ctgcagcgcc ggcggcagcg caacgcgacc accgaggacg atgaagctcg
      301 cgcgcggcag gtgcgttgct gcggcgatgc agagcagcgt gaaccacagc cagaagtcgc
      361 tgctcatatc ttgccttccg gcgtggccca gtgctcggcc gcaaacccgg cggcgatgcc
      421 ggcgacgatg gcgacgatca cgcccatgcg cagcggcagg cccgagagca gcacggcgac
      481 gactccgccg gtggtcgcgg caaccagcat cggccgcgcc ttgaccatcg gcgcgagcaa
      541 taccatcagc gcgatgcttg ccatgaactc gagcgaccag tccttcggaa tcgaatcggc
      601 ggcaagcacg ccgatcagcg cgaagacctg ccagagaatc caggtgaaga ttgacggcgc
      661 gaggtagtaa ccgagccgcc aggcccggtc ctcgacgtcg agcattttgt tcaggcagac
      721 cgcgaacacg ccgtcagtga gcaggtaacc cgagagccaa cgcagcggcg tcgatacacc
      781 acgaaatccg tggctgatcg cggcgctgaa gatgacgaag cgtaggttaa gcaccagcgc
      841 ggtaacgacg atcagccaca tcggtgcgcc gatggcgatc agcggcaacg tgccgatctg
      901 cgcggtgccg gcgaagacga tgatgttcat gcccatcgcc tgcaccggcg tcaggccggc
      961 gccgatcatt gcgatgccgg tgaccatggc ccaggggact aggccgatcg agagcggcag
     1021 gaagaccatg aatccctctc gggaaccggc gcggaagctt tcggacatcg gcatgcctgt
     1081 ggagtcggcg tcgcgaaacg aggcggcaaa cttatcacgc gcggcagcgc ccggcggcac
     1141 ccctcgctac aatgcgcgac ccatcatcga gccaatacag gagaaatcat gagcacgacg
     1201 accacggcca gcggcctcat tattgaagaa ctcgaagtcg gcagcggcga gacggctgcg
     1261 gctggaatgc acgtgcaggt gcactacacc ggctggctga ctgacggcac gaagttcgat
     1321 tcgagcaagg atcgcgacga gcccttcgat ttcccgctcg gcggccgacg cgtgattgcc
     1381 ggctgggacg agggcgtcgc cggcatgcag atcggcggca agcgcaagct gaccatcccg
     1441 ccgcagctgg gctacggcgc gcgcggtgcc ggtggcgtca ttccgccgaa cgccacgctg
     1501 atttttgagg tcgagttgct cgcggtcgag tgaggccgat cagtctcagc caaatagcgc
     1561 gtcgatgtcg atgtcgggct gaactgcctc gaccgtcgtc gactttagct ggccgccgga
     1621 aaccagacgg ccgtagccgc cgatgcggtt gcggccgttt tccttgcacc agtaaagggc
     1681 ggcgtcggcc tggccaagca gatcggtctg cgccgacatt gggtcgacga cggtgtagcc
     1741 gatgctgcag gtgacctggc cgacctgcgg aaacacgtgc ttttccacgg cctgccgaaa
     1801 gcggtcgagc gcgactttgg caccctcttc ggtggccgtg tgcagcatca cgacgaactc
     1861 ttcgccaccg aagcggaaga gcaggtcgta gccgcggaag ctctcgcgca tcaggttggc
     1921 gacgcggagc aggacttcgt cgccgaacag atgaccgaag ttgtcgttga cgcgcttgaa
     1981 gaagtcgatg tcgaccaccg ccatccacca gtccgatgga ctgtcacggc gtcgctcgac
     2041 cgaggacgcc gaatgctcgt cctgggtttt cgccagcagg cgctcaaagg cgtcgtcgaa
     2101 ggtcttgcgg ttgagcagcc cggtcagcgt gtcgaactcg gaatagtcga gcaggccgac
     2161 gtagttggcg tagatacgca cgaaccagct cagcgattcg atttcctcgg cggtcgccgc
     2221 acggcc
//
LOCUS       DATDAA010000217         9797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_260725,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000217 DATDAA010000000
VERSION     DATDAA010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260725"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /gene="epsC"
                     /locus_tag="VIS73_06855"
     CDS             <1..650
                     /gene="epsC"
                     /locus_tag="VIS73_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEY5762909.1"
                     /translation="LMHRLAHRLWIARWLWLARFIAHISRFLTGIEIHPGARIGRKFF
                     IDHGMGVVIGETAEIGDGVTLYHGVTLGGTSWNEGKRHPTLGDGVVIGAGAKVLGPIL
                     IGNNAKVGSNAVVFKEVPAGATAVGNPARVIDDEQAKQREEKAEKMGFSAYAVAENMD
                     DPLAKAIHGLLDHAVETDRRIEALLARLRAAGVNVDDGVRSRDEFDPQYLGKIVD"
     gene            706..1194
                     /gene="iscR"
                     /locus_tag="VIS73_06860"
     CDS             706..1194
                     /gene="iscR"
                     /locus_tag="VIS73_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEY5762910.1"
                     /translation="MRLTTKGRFAVTAMLDLAMRGGNGPVTLAGISERQRISLSYLEQ
                     LFGKLRRHGLVDSVRGPGGGYNLARPAKSITVADIIIAVDEPLDATQCGGKENCLDEH
                     RCLTHELWATLNRKMYDYLHSVSLGELERQADSKRERDDNKVSPVVDRRPAARRPMAA
                     ST"
     gene            1219..2367
                     /locus_tag="VIS73_06865"
     CDS             1219..2367
                     /locus_tag="VIS73_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEY5762911.1"
                     /translation="MTFSPINCDFNATTPLAPTVLQAMLPYLEAQCGNPSSRHEFGRA
                     ARRAVDEARESVAAAVGAHPTEIIFTSGGTEASNLLLKGAASLMRPGLLAVGASEHPS
                     VLEPARQLARSGWHLAELDVDSDGLIARSAVEAILAKRPVIASVMLANNETGVVQPLR
                     EIAGQLTGAGCTVHTDAVQALGKLPVDFRELGVSAMTLSAHKVGGPKGAGALVLSKRV
                     ELQPLLAGGGQERELRSGTENVPAIVGFGRACELLAQRQLTQRKLEALRDCLEKDLCA
                     AGATIFGAAAPRLANTSFFAFSGIDGETLVGKLDRAGFATASGSACSSAKPGVSHVLL
                     AMGVAEELARGAVRVSFGPETRDAEVAALAETIARTVSGLRATASVLA"
     gene            2438..3646
                     /locus_tag="VIS73_06870"
     CDS             2438..3646
                     /locus_tag="VIS73_06870"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005090087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEY5762912.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEAMIPWLTEHFGNPASRSHSFGWE
                     AEAAVEKAREQVAALVNADPKEIVWTSGATESNNLAIKGAAHFYASKGKHLITLKTEH
                     KAVLDTVRELERQGFEATYLDVQEDGLVDLEAFKAAIRPDTSVASVMLVNNEVGVIQP
                     IAEIGEICREKGVIFHVDAAQATGKVDIDLQALKVDLMSFSAHKTYGPKGIGALYVRR
                     KPRVRLEAQMHGGGHERGFRSGTLATHQIVGMGEAFRIAREEMHTENERIRMLRDRLL
                     RGLSTMEATFVNGDMERRVPHNLNISFAYVEGESMIMAVKDLAVSSGSACTSASLEPS
                     YVLRALGRDDELAHSSIRFTIGRFTTVEEVDFAIKLLQEKVGKLRELSPLWEMFQDGV
                     DISAVQWAAH"
     gene            3702..4088
                     /gene="iscU"
                     /locus_tag="VIS73_06875"
     CDS             3702..4088
                     /gene="iscU"
                     /locus_tag="VIS73_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEY5762913.1"
                     /translation="MSYSVKVLDHYENPRNVGSFAKDEEGVGTGMVGAPACGDVMKLQ
                     IRVNREGVIEDAKFKTYGCGSAIASSSLVTEWVKGKTVDQALDIKNTQIAEELALPPV
                     KIHCSILAEDAIKAAVADYKKKRAED"
     gene            4091..4414
                     /gene="iscA"
                     /locus_tag="VIS73_06880"
     CDS             4091..4414
                     /gene="iscA"
                     /locus_tag="VIS73_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEY5762914.1"
                     /translation="MPVTLTERAAQHVSRFITKRGKGVGLRLGVRTSGCSGMAYKLEF
                     ADEAQPDDCRFESHGVTVLIDPKSLPYLEGTELDYAREGLNEGFKFNNPNVKDACGCG
                     ESFKV"
     gene            4465..4989
                     /gene="hscB"
                     /locus_tag="VIS73_06885"
     CDS             4465..4989
                     /gene="hscB"
                     /locus_tag="VIS73_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEY5762915.1"
                     /translation="MAFATNHFTLFGLPQSFAIDPAALDSRYRAVQAEVHPDRFAAEG
                     EAERRRAVQLSAQVNEAYQTLRAPLARARYLLGLRGVDAGVESNTAMPTDFLMAQMEW
                     REQFEEARAERKVEALEALLDRLREQLDAGYQALAVSLDSSGDLVAASNTVRELMFVD
                     RLREQVNDVLAEIE"
     gene            5082..6962
                     /gene="hscA"
                     /locus_tag="VIS73_06890"
     CDS             5082..6962
                     /gene="hscA"
                     /locus_tag="VIS73_06890"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEY5762916.1"
                     /translation="MALLQIAEPGESAAPHQHRLAAGIDLGTTNSLVATVRSGMAAVI
                     NDEEGRPLLPSVVRYLADGRPVVGTAAQNMQSDDPRNTVVSVKRFMGRSLRDIDGADS
                     VPYEFIDSPGMLRIKTVAGAKSPVEVSAEILRVMKDRAEEALGGPLVGAVITVPAYFD
                     DAQRQATKDAAKLAGLNVMRLLNEPTAAAIAYGLDNGSEGVYAVFDLGGGTFDISILR
                     LSKGVFEVLATGGDSALGGDDFDQRILCWVIEEAKLQPLSPRDARLLLVRAREAKEYL
                     TQHGEAPISARLSTGEIVDLRLTSAIFADISQTLVAKTLQPVKRALRDAGLRVDDVEG
                     VVMVGGATRMPQIQKAVAAYFRKSPLNNLDPDKVVALGAAIQANVLAGNRSADDDWLL
                     LDVIPLSLGLETMGGLVEKVIPRNSTIPVARAQEFTTFKDGQTAMAIHVVQGERELVD
                     DCRSLARFELRGIPPMVAGAARIRVTFQVDADGLLSVYAREQTKGVEASVSVKPAYGL
                     SDSEIAGMLEDSMAHAKDDAFLRALRESQVEARRLIEATESALEEDGALVDAGERQLI
                     EREIAALRALVDGDNRRQINLAMDDLSAATQDFAARRMDQSVRRALAGRAVSEISEAE
                     GR"
     gene            6959..7300
                     /gene="fdx"
                     /locus_tag="VIS73_06895"
     CDS             6959..7300
                     /gene="fdx"
                     /locus_tag="VIS73_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEY5762917.1"
                     /translation="MTRVIILPHPEVCPEGTVLEVEPGESLCEAMLAKDIEIEHACEM
                     SCACTTCHVVVREGFESLPEASEDEEDMLDKAWGLERQSRLSCQVVVGDTPLTIEIPR
                     YTINLASEGKH"
     gene            7314..7508
                     /gene="iscX"
                     /locus_tag="VIS73_06900"
     CDS             7314..7508
                     /gene="iscX"
                     /locus_tag="VIS73_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEY5762918.1"
                     /translation="MKWTDVYDIAAELEDTHPEVDPMRINFVDLMAWVQALPGFDDDP
                     KRCGERILEAIQAAWIEERN"
     gene            7508..8155
                     /locus_tag="VIS73_06905"
     CDS             7508..8155
                     /locus_tag="VIS73_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5762919.1"
                     /translation="MGLEYLVVPVTNFEQNCTLLWCASTRKAVVVDPGGDVPKIQKAI
                     ADRKLSLDRILITHGHVDHAGGAADLAKAMAVPIWGPHEADGFWIEAMERQSQMFGLP
                     GARRFTPDRWLAEGDCVEFGEVSLEVLHCPGHTPGHVIFFDRAARLAQVGDVLFRGSV
                     GRTDFPGGDHDDLMSSIRNKLWLLGDDVSFISGHGPMSTFGAERASNPFVGAQVA"
     gene            8192..8833
                     /locus_tag="VIS73_06910"
     CDS             8192..8833
                     /locus_tag="VIS73_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEY5762920.1"
                     /translation="MTKRQGGAALPDFWAEASRVLAANDPVMASMVKRYQGRGLASRG
                     DPFATLMRSVVGQQISVRAADAVWGRLMEALGAPDAEAVLGSDATILRACGLSYRKIE
                     YVQDLAGRFADGRIDPTRWRGLADSEIIAELTETRGIGAWTVEMLLIFNLMRPDVWPV
                     DDKGLQKAVALHYLDGRVPPRKELLAIGERWRPWRSVATWYLWRSLDPVPVEY"
     gene            8930..>9797
                     /locus_tag="VIS73_06915"
     CDS             8930..>9797
                     /locus_tag="VIS73_06915"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY5762921.1"
                     /translation="MKTSFLDFEQPIADLENKIEELRFMQEDSAVDITEEISRLERKS
                     QSLTKDIYGKLSSWQIAQVARHPQRPYTLDYIERIFTDFHELHGDRSFADDPAIVGGL
                     ARFNGQSVMVIGHQKGRDTKEKIARNFGMPRPEGYRKALRLMRLAERFAIPVITFVDT
                     PGAYPGIGAEERGQSEAIGRNLYVMAELKVPIIVTVIGEGGSGGALAIAVGDTVQMLQ
                     YSTYSVISPEGCASILWKSADKAADAAEIMGITAPRLKTLGLIDKVVAEPCGGAHRDH
                     AAMAQSLKKSLQD"
BASE COUNT         1787 a         2992 c         3300 g         1718 t
ORIGIN      
        1 tgctgatgca ccgcctcgcg caccggctgt ggattgcacg ctggctctgg ctggcccggt
       61 tcatcgcgca catctcgcga ttcctgactg gcatcgaaat ccatccgggt gcccgcatcg
      121 gccgcaagtt tttcatcgac catggcatgg gtgtggtcat cggcgagacc gccgagattg
      181 gcgacggcgt gacgctctat cacggcgtca cgctcggcgg caccagctgg aacgaaggca
      241 agcggcatcc gaccctgggc gatggtgtcg tcattggcgc cggcgccaag gtcctcggcc
      301 cgatcctgat cggcaacaac gccaaggtcg gatccaatgc ggtcgtgttc aaggaagtgc
      361 cggcgggcgc gaccgccgtc ggcaatccgg cgcgggtcat cgacgacgaa caggcgaagc
      421 agcgcgaaga gaaggcggag aagatgggtt tttcggccta cgccgttgcc gaaaatatgg
      481 acgatccact ggccaaggca attcatgggc tgctcgatca cgcagtcgag accgaccggc
      541 ggatcgaggc gctgctggcc cgtctcaggg cggccggcgt gaacgtcgac gacggggttc
      601 gctcgcgcga cgaattcgat ccgcagtacc tgggcaaaat agttgactaa aaaagtcggc
      661 attggctata cttgaccgca acagtcgggt atcgagaggt ggcaaatgcg tttgacgacc
      721 aagggaagat tcgcggtaac ggccatgctg gatctcgcca tgcgcggcgg caacggtccg
      781 gtgacgctgg ccggcatcag cgagcggcag cggatttcgc tgtcgtattt ggagcagttg
      841 ttcggcaagc tgcgccggca cgggttggtt gacagcgttc gtgggcccgg cggcggctac
      901 aacctggcgc gtccggcgaa atcgatcacg gttgccgaca tcatcatagc tgtcgatgag
      961 ccgctcgatg cgacccagtg cggcggcaag gaaaactgtc tcgacgagca tcgttgcctg
     1021 acccacgagc tgtgggcgac gctgaatcgc aagatgtacg actacctgca ctcggtgagc
     1081 cttggcgaac ttgagcggca ggcggattcc aagcgcgagc gcgacgacaa caaggtctcg
     1141 ccggttgtcg accgtcgccc cgcggctcgg cgcccgatgg cagcgagtac ctgacttccc
     1201 ctcggcagcc tgaccaaaat gaccttctcg ccgatcaact gcgatttcaa cgcgacgacg
     1261 ccgctggcgc cgaccgtgct gcaggcgatg ctgccgtatc tggaggcgca gtgcggcaat
     1321 ccgtcgagtc gtcacgagtt cggccgggct gcccggcgcg ccgtcgacga ggcgcgcgaa
     1381 tcggttgctg ctgcagtcgg tgcgcatccg accgagatca tctttacgag cggcggcacc
     1441 gaagcgagca atctgctgct caagggtgcg gcatccctga tgcggcccgg tttgctcgcc
     1501 gtcggtgcaa gcgagcatcc gagcgtgctc gagccagcgc gccagttggc gcgaagtggc
     1561 tggcatttgg cggaactcga cgtcgacagc gatggcttga tcgcgcgcag tgccgtcgag
     1621 gcgattctgg cgaagcgtcc ggtgatcgcc tcggtgatgc tcgccaataa cgaaaccggc
     1681 gtcgtccagc cgctgcgcga gatcgccgga cagcttaccg gggccggttg cacggttcat
     1741 acggatgcgg tgcaggcgct gggcaagcta ccggtcgatt tccgcgagtt gggcgtcagc
     1801 gcgatgacgc tctcggcaca caaggtcggt ggacccaagg gcgcgggcgc tctggtgctg
     1861 agcaagcgtg tcgagttgca gccattgctt gctggtggcg ggcaggagcg cgagctgcgc
     1921 tcgggaaccg aaaacgtccc ggcgatcgtc ggctttggcc gtgcctgcga actgctggcg
     1981 cagcgacagc tgacgcagcg aaagctcgaa gcgttgcgcg attgtctgga aaaggacctg
     2041 tgcgccgcgg gcgcgacgat ctttggcgcc gcggcgccgc gactggcgaa taccagtttt
     2101 tttgcctttt ccggcatcga cggcgagacg ctggtcggca agctcgaccg tgcgggtttc
     2161 gcgacggcga gcgggtccgc gtgttcaagc gcgaaacccg gcgtgtcgca tgtcctgctg
     2221 gcaatgggtg tggccgagga actcgcgcgc ggggcggttc gcgtgagttt cggccccgag
     2281 acgcgcgacg ccgaagtggc ggcgctggcg gagacgattg ctcgtaccgt cagtggattg
     2341 cgcgccacgg catcggtcct ggcatgaatg aatacggtta cgaaccaagc gtcggataga
     2401 cggtcgatga ggccatacga atgattagga gaaacacatg aagctgccga tttatctgga
     2461 ctactccgca accacgccgg tcgatccgcg cgttgccgag gcgatgattc cctggctgac
     2521 cgagcatttc ggcaacccgg catcgcgttc gcacagcttc ggctgggagg ccgaggcagc
     2581 ggtcgagaaa gcccgcgaac aggtggcggc gctagtgaat gccgacccca aggaaatcgt
     2641 ttggacctcc ggcgccacag aatcgaacaa cctggcgatc aagggcgcag cgcatttcta
     2701 cgccagcaag ggcaaacacc tgatcaccct caagactgaa cacaaggcgg ttctcgacac
     2761 cgttcgcgaa ctcgagcggc agggcttcga ggcgacctac ctcgacgttc aggaagacgg
     2821 gctggtcgat ctcgaggcct tcaaggccgc gattcgcccc gacacgtcgg tggcctcggt
     2881 gatgctggtc aacaacgagg tcggcgtgat ccagccgatc gctgagatcg gcgagatctg
     2941 ccgggagaag ggcgtgatct ttcacgtcga tgcggcgcag gcgaccggca aggtggacat
     3001 cgacctgcag gcgctcaagg tcgacctgat gagcttctcc gcgcacaaga cctacggtcc
     3061 gaagggcatc ggtgcgcttt acgtccggcg caagccgcgc gtgcgcctgg aagcgcagat
     3121 gcacgggggt ggtcacgagc gcggtttccg gtcgggcacc ttggcgaccc accagatcgt
     3181 cggcatgggc gaagcgttcc gcattgcccg cgaggaaatg cacaccgaga acgagcggat
     3241 ccgcatgttg cgtgatcgct tgctgcgggg cctgtcgacg atggaagcga ccttcgtcaa
     3301 cggcgacatg gagcggcgcg tcccgcacaa cctcaacatc agcttcgcct atgtcgaggg
     3361 cgagtcaatg atcatggcgg tcaaagatct cgcggtatcg agcggctcgg cctgtacctc
     3421 ggcgagcctg gagccttcct acgtgctgcg ggcattgggc cgcgacgacg agctggcgca
     3481 cagctcgatc cgcttcacca tcggccggtt cacgacggtc gaggaagtcg acttcgcgat
     3541 caagctgctg caggagaagg tcggcaagct gcgcgaactg tcgccgcttt gggagatgtt
     3601 ccaggacggc gtcgatatat ctgcggtgca gtgggccgca cactgaattt tcgaccggca
     3661 agccggtcct acgaacaggg caacctcttc aggagaagca aatgagctat agcgtaaaag
     3721 tactcgacca ctacgagaat ccgcgcaacg tcggttcctt cgccaaggac gaggaaggcg
     3781 tcggtaccgg gatggttggc gcgccggcct gcggtgacgt catgaagcta cagattcggg
     3841 tgaatcgcga gggtgtcatc gaggatgcca agttcaagac ctacggctgc ggatcggcga
     3901 ttgcatcgag ttcgctggtg accgagtggg tcaagggcaa gaccgtcgat caggcgctcg
     3961 acatcaagaa cacgcagatc gccgaggaac tcgcgttgcc gccagtgaaa atccactgtt
     4021 cgatcctcgc cgaggacgcg attaaggccg ccgtggccga ttacaaaaag aagcgcgcgg
     4081 aggattgagc atgccagtca cgctgaccga acgcgcggcg cagcacgtgt cgcgcttcat
     4141 taccaaacgc ggcaagggtg tcggtttgcg gcttggtgta cgcaccagcg gctgctcggg
     4201 aatggcctac aagctcgagt tcgccgacga ggcgcaaccc gacgattgtc gtttcgagag
     4261 ccatggcgtg accgtgctga tcgacccgaa aagcctgccc tatctcgagg gtaccgaact
     4321 cgactatgcc cgcgaagggc tcaacgaggg cttcaagttc aacaatccga acgtcaagga
     4381 cgcctgcggc tgcggcgaat cgttcaaggt ctgaggccgg cgacgatgca tctgcttccc
     4441 ggctcgcgcc gatcgcctgc ctgaatggcg ttcgcgacga accatttcac gctgttcggc
     4501 ttgccgcagt ccttcgcgat cgatcctgcg gcgctcgatt cccgctaccg tgccgtgcag
     4561 gccgaggtgc acccggatcg ttttgccgcc gagggcgaag ccgagcgacg gcgagccgta
     4621 cagctttcgg cgcaggtcaa cgaggcctac cagacgctgc gtgcgccgct ggcgcgtgcc
     4681 cgctacctgc tcgggctgcg gggcgtcgat gccggcgtcg aatcgaatac ggcgatgccg
     4741 accgacttcc tcatggcgca gatggaatgg cgcgagcaat ttgaggaagc gcgcgccgaa
     4801 cgcaaggtcg aggcactgga agctttgctc gaccgcctgc gcgagcagct cgacgccggc
     4861 tatcaggcgc ttgccgtctc gctcgattcc agcggggatc tggtggccgc gtcgaacacc
     4921 gtgcgcgagc tgatgttcgt cgaccgcctg cgcgagcaag tgaacgacgt gctcgccgag
     4981 atcgagtagc acgccgacgt tctcgcgtaa catccggccg gcgccggcct gcagaggccg
     5041 cgccgaccgt catcgactga ccaaaataga cagaatgaac gatggcctta ctgcaaatcg
     5101 ctgaacccgg agaatcggct gccccgcacc agcatcggct ggcggcggga attgaccttg
     5161 gaacgaccaa ctcgctggtc gccacggtcc gcagcggcat ggcggcggta atcaacgacg
     5221 aggagggcag gcccctgctg ccctcggtgg tccgatatct ggccgatggc cggccggtcg
     5281 tcggtacggc ggcgcagaac atgcaatccg acgatcctcg caatactgtg gtttcggtca
     5341 agcgtttcat gggcaggagc ctgcgcgaca tcgatggcgc cgactcggtg ccctacgaat
     5401 tcatcgattc gccgggcatg ttgcggatca agaccgttgc cggggccaag agtccggtcg
     5461 aggtctccgc ggaaatcctg cgggtgatga aggatcgcgc cgaagaggcg ctcggtgggc
     5521 cgctggttgg cgctgtgatc acggtgccgg cgtatttcga tgatgcgcag cggcaggcga
     5581 ccaaggatgc ggcgaagctc gccggcctta acgtcatgcg actgctcaac gagccgactg
     5641 ccgcggcgat cgcctacgga ctcgacaatg gttcggaagg cgtctatgcg gtgttcgacc
     5701 tcggtggcgg caccttcgat atttccatcc tgcggctttc caagggggtc ttcgaggtgc
     5761 tcgcgaccgg cggcgattcg gcgctgggcg gcgacgattt cgaccagcgg attctctgct
     5821 gggtgatcga ggaggcaaag ctgcagccgt tgtcgccgcg cgatgcccgg ctgcttttgg
     5881 tgcgggcgcg cgaagccaag gaatacctca cgcagcacgg cgaggcgccg atctcggcgc
     5941 ggttgtcgac gggcgagatc gtcgacctgc ggctgacctc ggcgatcttc gccgacatct
     6001 cgcagacgct cgttgccaag acgctgcagc cggtcaagcg cgccttgcgc gatgccggcc
     6061 tgcgcgtcga cgacgtcgag ggcgtggtga tggtgggcgg cgcgacgcgc atgccgcaga
     6121 tccagaaggc tgtagccgcc tactttcgca agagcccgct gaacaacctc gacccggaca
     6181 aggtggtggc gctcggcgcg gcaatccagg ccaacgtgct tgccggcaac cgcagcgccg
     6241 atgacgactg gctgctgctc gatgtcattc cgctctcgct ggggctggaa acgatgggcg
     6301 gcttggtcga aaaggtcatc ccgcgcaatt cgacgatccc ggttgcccgc gcgcaggaat
     6361 tcacgacctt caaggacggc cagacggcga tggcgatcca cgtcgtgcag ggagaacgcg
     6421 aactggtcga cgactgtcgg tctttggcgc gctttgaatt acgtggaatt ccgccgatgg
     6481 tcgccggtgc ggcgcgcatt cgtgtgacct tccaggtcga cgccgacggg ctgctctcgg
     6541 tatatgcccg cgaacagacc aagggggtcg aggcctcggt ttcggtcaaa ccggcctacg
     6601 ggctaagcga ttcggaaatc gccgggatgc tcgaggattc gatggcccac gcgaaggatg
     6661 acgcgttcct gcgggcgctg cgcgaatcgc aggtcgaggc ccgccgcctg atcgaggcga
     6721 ctgaatcggc gctggaagag gatggcgcgc tggtggatgc cggcgagcgg cagctgatcg
     6781 aacgggaaat cgcggcgctg cgggcgctgg tcgatggcga caatcgtcgc cagatcaatc
     6841 tggcgatgga cgatctttcg gcggcaaccc aggacttcgc cgcccggcgg atggaccaga
     6901 gcgtccgtcg ggcgctcgcg ggccgcgccg tatccgaaat cagtgaggcg gagggaagat
     6961 gactcgcgta atcatcctgc cgcacccgga agtctgtccc gagggcaccg tgcttgaggt
     7021 cgagcccggc gagtcgctgt gcgaggcgat gctcgccaag gacatcgaga tcgagcacgc
     7081 ctgtgagatg tcctgcgcct gcaccacctg ccacgtcgtc gtccgcgagg gttttgaatc
     7141 gctgcccgag gccagcgagg acgaagaaga catgcttgac aaggcctggg ggctggaacg
     7201 ccagtcgcgg ctcagttgcc aggtggtcgt cggcgatacg ccgctgacca tcgaaattcc
     7261 acgctacacc atcaatcttg cctccgaggg caagcactga aacggaggct gccatgaaat
     7321 ggacggatgt ctacgatatc gccgccgaac tcgaggacac gcatccagag gtcgatccga
     7381 tgcgcatcaa ctttgtcgac ctgatggcct gggtgcaggc cttgcccggc ttcgacgatg
     7441 atccgaagcg ctgcggcgag cggatcctcg aggcgattca ggccgcctgg atcgaggagc
     7501 gcaactgatg ggtcttgagt acctcgtcgt cccggtcacc aacttcgaac agaactgcac
     7561 gctgctctgg tgcgccagta cgcgcaaggc ggtcgtcgtc gatcccggcg gcgatgtgcc
     7621 gaagatccag aaggccattg ccgaccgcaa gctgtcgttg gacaggattc tcatcaccca
     7681 cgggcacgta gatcacgcgg gtggcgccgc cgatctggcg aaggcgatgg ccgtgccgat
     7741 ctgggggccg cacgaggccg atggcttctg gatcgaggcg atggaacggc agagccagat
     7801 gttcggtctg cccggagcgc gccgcttcac ccccgaccgc tggctcgccg aaggcgactg
     7861 cgtcgagttc ggtgaggttt cgctggaggt gctgcactgc ccgggccaca cgcccggcca
     7921 tgtgatcttc ttcgaccgtg cggcgcggct cgcgcaggtc ggcgacgtgc tgttccgcgg
     7981 ctcggtcggc cgtaccgatt tccccggtgg cgatcatgat gacctgatgt cctcaatccg
     8041 caacaagctc tggctgctcg gcgacgacgt ttccttcatt tcgggacacg ggccgatgtc
     8101 gaccttcggc gcggagcgcg ccagcaatcc cttcgtcggg gcgcaagttg cctgatagct
     8161 gcgtaacagg tgccagcagg cccgcggacg cgtgaccaag cgccagggtg gcgccgcgct
     8221 gcccgacttt tgggccgagg cgtcacgggt cctcgctgcg aacgatccgg tgatggcgtc
     8281 gatggtcaag cgttatcagg ggcggggact cgccagccgt ggcgatccgt ttgcgacgct
     8341 tatgcgctcg gtggtgggcc agcaaatctc ggtgcgcgcc gccgacgcgg tgtgggggcg
     8401 gctgatggag gcccttggcg cgcccgatgc ggaggccgtg ctgggcagcg atgcgacgat
     8461 cttgcgtgcc tgcggcctgt cctatcgcaa gatcgagtac gtccaggacc tcgctggccg
     8521 tttcgccgac ggacgtatcg acccgacgcg ctggcgcggg cttgctgatt ccgagatcat
     8581 cgccgagctg accgagaccc gtggcattgg tgcttggacc gtcgagatgc tgctgatttt
     8641 caacctgatg cgccccgacg tctggcccgt tgatgacaag ggcctgcaga aggccgtcgc
     8701 gctgcattac ctggacgggc gcgtaccgcc acgcaaggag ctgctcgcaa tcggcgagcg
     8761 ctggaggcct tggcgttcgg tcgccacctg gtatctctgg cgctcactcg atccggttcc
     8821 ggtcgaatac tgattccgtt ctccgccggg taccgagtct ggcggccgga ttgccgattg
     8881 gcaaactgcg gtagaattcg cgtccccacc tgcggcaaag attccccgaa tgaagaccag
     8941 cttcctcgat ttcgagcagc cgatcgccga ccttgaaaac aagatcgaag aactccgttt
     9001 catgcaggag gattcggccg tcgacatcac cgaagagatt tccaggctgg aacgcaagag
     9061 ccagagcctg accaaggaca tctacggcaa gctcagttcc tggcagatcg cgcaggttgc
     9121 gcgacacccg cagcgaccct acacgcttga ctacatcgag cggatcttta ccgatttcca
     9181 tgagctgcac ggcgaccgtt cgttcgcaga cgatccggcg atcgtcggcg ggctggcgcg
     9241 ctttaacggc cagtcggtga tggtgatcgg ccaccagaag gggcgcgata ccaaggaaaa
     9301 gatcgcccgc aacttcggca tgccgcggcc tgagggctat cgcaaggcct tgcgcctgat
     9361 gcgcctggcc gagcgcttcg ccatcccggt catcaccttc gtcgatacgc cgggcgccta
     9421 cccgggaatt ggcgccgaag aacgcggcca gtccgaggcg atcggccgca acctctacgt
     9481 gatggccgaa ctgaaggtgc cgatcatcgt cactgtgatc ggcgagggcg gttccggcgg
     9541 cgcgctagcg attgccgtcg gcgataccgt acagatgctg cagtactcca cttactcggt
     9601 catctcgccc gagggctgtg cttcgatcct ctggaagagt gccgacaagg ccgccgatgc
     9661 agctgagatc atgggcatca ccgcgccgcg gctgaaaacg ctcggcctga ttgacaaagt
     9721 cgttgccgaa ccctgtggcg gcgcgcaccg cgaccatgcg gcgatggcac aatcactgaa
     9781 gaagtcgctg caggatg
//
LOCUS       DATDAA010000218         8799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_330729,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000218 DATDAA010000000
VERSION     DATDAA010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8799
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330729"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VIS73_06920"
     CDS             complement(<1..235)
                     /locus_tag="VIS73_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY5762922.1"
                     /translation="MTPKVRKFVWQESFSVGDATIDAQHRRIIEILNLLYDLLHSDAV
                     GEDLTNGLREVFWELHSYVAEHFAYEEQRMADAN"
     gene            complement(255..1127)
                     /locus_tag="VIS73_06925"
     CDS             complement(255..1127)
                     /locus_tag="VIS73_06925"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY5762923.1"
                     /translation="MNQPLLSLPIRLFALLAATALVVGCKTTDLDFAKVASVTQNLAR
                     VVAPGKLTPEEEEVMGRDTAATLLGAAPLVKDPALQAYVNRVGSWIALQTGRDDIRWR
                     FGVIDTPNINAFAAPAGYVLVTRGLLARLDDESSLAGVLAHEIAHVVRRHHAEAMAKK
                     DRAGALAGLADDLNKSRNKDSIGKLANLAKGVYSAGLDKDDEYEADRLGIVYATRAGY
                     QPYGLPQVLQMYAGAAGESGFELLFSTHPTPQERLDALDVAMGDRLARYESESVDDTP
                     SFRRYIAARKTALR"
     gene            complement(1141..1650)
                     /locus_tag="VIS73_06930"
     CDS             complement(1141..1650)
                     /locus_tag="VIS73_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY5762924.1"
                     /translation="MRSLSLLRPALAAIALSLLAATVAATPATVVRNSTAHERPFVDS
                     PTVAMLATKTRVEIIGAQGAWNQVRLSNGRTAWVRLLNLRPEAARGGSSLAGIGQLGN
                     VARTGSTGSAATTGAKGISKEELESAEPNLAEVERLEGFRSNPAEARQYAASARLQAR
                     EVAPLPERK"
     gene            complement(1647..3446)
                     /locus_tag="VIS73_06935"
     CDS             complement(1647..3446)
                     /locus_tag="VIS73_06935"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5762925.1"
                     /translation="MSTAAAAALLAVLALANDWLYALDGRAGDWLLRQHAAQRPPPTD
                     IVVVDIDQKSLDDPRMLEIAGNWPWPRAVHGELLAALAAGHPKAVVFDLIFSEPDVFR
                     PESDAFFSEMLRQNPAYLPLVIAGDGTGSRLADLPPQMGIRATPQADLDASLPLIAPK
                     ALDVDVWRTGIINFLEDADGIGRRYWLFRPQRGWETPSIAARVAADAGWALPAAAAIR
                     LNWYARPFTRISYADYYLESLRESPSGLADVSGKIVIVGAGAPGLHDLRPTPLGATTP
                     GPDILATAIANLADGDALRPLAPAAGIALTLALIATTTLAFLRHLSPAMTAAALAAAS
                     AVTVGSAWLMLQSGIEWRPYSAIAGVWLAFVGIAIVAHLQERQSREHAVQMFGRFLDP
                     RVVHTLTNEGEIAAAQAGRSQEITVLFSDIRGFTTLSETRSPEEIVRILNRYFDLQVE
                     AIFASDGTLDKFIGDAIMAFWGAPLPRAEHAALAVEAALAMADKLIEFKAELGELGEN
                     FDIGIGVHSGPAVVGFLGSSQRLDYTAIGDTVNLASRIEGQTKGIARVLVSDATRIAC
                     EACSQTFEFIDHGEFTVKGRAQAVRLFEPRRKP"
     gene            3611..4423
                     /gene="pcaD"
                     /locus_tag="VIS73_06940"
     CDS             3611..4423
                     /gene="pcaD"
                     /locus_tag="VIS73_06940"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR02427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HEY5762926.1"
                     /translation="MSIKINGVEVAFDSIDEAGPAAPIITFAHCLGGDRTIWRKQLAH
                     WRSRYRLLPYDMRGQGGSTATPGPYSMEQLAADALALLDAQGVERTIFCGVSMGGMVA
                     LQLALLAPQRVAALVLADSAGGFGAEARAAWTERLAAVERDGVAPLAETMMGRWFTDG
                     FRQRAVGEVAEVAAALARTPVAGYLGACAAIRDFDLRERLREIECPTLVLCGEHDPST
                     PLALSQELAAGIPGARLEVLAGANHLPNFEQPERFNAVVDRFLASLGPDLAL"
     gene            4486..5463
                     /locus_tag="VIS73_06945"
     CDS             4486..5463
                     /locus_tag="VIS73_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY5762927.1"
                     /translation="MTQAIRIHKTGGPDVLQWEEVKVGDPGPGEARVRHEAVGLNYID
                     TYHRTGLYPLPALPSGIGLEGAGVVEAVGSGVEGLKAGDRVAYAGGPPGAYSEARCLP
                     ADRLLKLPKGIDSRTAAGMMLQGLTTAYLLRKTYAVQPGDAVLIHAAAGGVGLIACQW
                     AKALGAMVIGTASTEEKAELARQHGADHVILYSKEAFAPRVRELTGGEGVAVVYDGVG
                     KDTFAGSLDSLRVRGMLVAYGNASGPVPPFDPLLLSQKGSLFLTRPTLMHYTAQRAEL
                     AELGGELFDVVGRGAVRIEVRQTYALKDAAMAHRDLEGRKTTGSTILLV"
     gene            5512..6135
                     /locus_tag="VIS73_06950"
     CDS             5512..6135
                     /locus_tag="VIS73_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY5762928.1"
                     /translation="MQVTVVANPKGGVGKSTLATNLAGFFARRGESVMLGDVDRQQSA
                     RDWLALRPAELPPIAGWEIDRNAPARPPKGTTQAVLDTPAGLHGKLLAKTLRVATRVV
                     IPLQPSLFDILATRHFLDALVEERAFRKGQLAVGVVAMRVDPRTRAAAELDRFLAQTE
                     VPVVAHIRSTQLYVQLAARGLTLFDVNPNPVAQDLEQWDSLIRWLDS"
     gene            complement(6068..6706)
                     /locus_tag="VIS73_06955"
     CDS             complement(6068..6706)
                     /locus_tag="VIS73_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004384134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HEY5762929.1"
                     /translation="MNYLAHALLADFAAPLRAGSATGDFFKGPLAPDLPADFARGIRL
                     HRAIDRYADSHPAFRASRTRLPANIRRWSGVIVDLYYDHILARQWADWHPQPLTEFSQ
                     SLYHEIAAYRHLMDEGAQGACRLMAREDWLSGYSTAAGLATTLQRMGRRVRRENPLAG
                     ADVHLGGDAAGFADDCRAFLVDARAFAVDWLSCQASESANPTAPDPAPPDSD"
     gene            6763..7896
                     /locus_tag="VIS73_06960"
     CDS             6763..7896
                     /locus_tag="VIS73_06960"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY5762930.1"
                     /translation="MRDRVAQWLRASPVSVALALVLLTVYLASAFRAGVVWEIPADAL
                     LRDGASYGPALAAGEWWRVVSAAFLHGGALHLLVNLVVLADAAPLVEAGGATADRPPP
                     LLTRLRRVIGVFIVTAAGGFVASAWWSSGAVSIGASGGILGFYGYWLARVWRMAGQRA
                     DQPRRFGPVLARRHVALAAGYLAAAVTAGFLIPGVDNAAHIGGLLGGAVLGWMPTANA
                     RASTAVAQLVLVSAALLAMPAQWAADYRAQQHFNEIYSRFVERDRQANAEIRRLLGEI
                     RAGRDPAAVAGEFDRLVAGRLRENRQLWLDQSADDGSSALAIERQRWQRYGDLRVDAA
                     ESLVAALLAPDGATQARELGRFERAMREARAIAEEANSALDKP"
     gene            7964..8476
                     /locus_tag="VIS73_06965"
     CDS             7964..8476
                     /locus_tag="VIS73_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HEY5762931.1"
                     /translation="MTQYQWDDRLLTGFEVVDSQHKRLFSLLQGLGDSLASSETDVEP
                     LQRAADALTHYAQEHFDDELELMQKQRCDLRHVMMHVQEHGAFIRRVSLFQESLAEGN
                     ASGRAKAGELVQFLVEWLKHHIRVVDQVMARQLRRIADGASPSDAYTAEVAEHPDARA
                     AHAFAEAPKD"
     gene            8538..>8799
                     /locus_tag="VIS73_06970"
     CDS             8538..>8799
                     /locus_tag="VIS73_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY5762932.1"
                     /translation="MLAPRFVHLRLHSEYSITDGMVRLDVGKNHDCPPVRRATEDGMP
                     AVALTDLANLFGMVKFYSAARAAGVKPVVGADVWVNDPEADRP"
BASE COUNT         1334 a         2841 c         3126 g         1498 t
ORIGIN      
        1 agttcgcgtc ggccatgcgc tgttcttcgt aggcgaagtg ctcggcgaca tagctgtgca
       61 gctcccagaa gacttccctg agcccgttag taaggtcctc gccgactgcg tcggagtgca
      121 gcaggtcgta gagcaggttg aggatctcaa tgatccgccg gtgctgcgcg tcgattgtcg
      181 cgtcgcccac cgagaaactc tcctgccaga cgaacttgcg taccttgggg gtcatggaaa
      241 gcctttcgtg atggtcagcg cagcgcggtc ttccgcgcag cgatgtaacg gcggaagctc
      301 ggcgtatcgt cgacgctttc cgactcgtag cgcgccaacc gatcgcccat ggcgacgtct
      361 agcgcatcga gccgctcctg cggggtcggg tgggtcgaga acagcagctc gaatcccgac
      421 tcgccggccg cgcccgcgta catctgcagc acctgcggca ggccgtaggg ctgatagccg
      481 gcgcgcgttg cgtagacgat gccgaggcgg tcggcctcgt actcgtcgtc cttgtcgagg
      541 ccggccgaat agacgccctt ggccagattg gcgagtttgc cgatcgaatc cttgttccgg
      601 ctcttgttga ggtcgtcggc taatcccgcc agcgcaccgg cgcggtcctt cttcgccatc
      661 gcttcggcgt ggtggcgacg gacgacgtgg gcgatttcgt gggcgagcac gccggccagt
      721 gacgactcgt cgtcaagtcg tgcgagcagc ccgcgggtga ccagcacgta gccggccggc
      781 gcggcgaagg cattgatatt cggtgtgtcg atgacgccga agcgccagcg gatgtcgtcg
      841 cgcccggtct gcagcgcgat ccagctgccg actcggttga cataggcctg cagcgccgga
      901 tccttgacca gcggtgccgc gccgagcagc gtcgccgccg tgtcgcggcc catcacttct
      961 tcctcttcgg gagtcagctt gcccggtgcg acaacgcggg ccaggttctg cgtcaccgag
     1021 gcgaccttgg cgaaatcgag atcggtggtc ttgcagccga cgaccaaggc cgttgcggcc
     1081 aggagtgcga acaggcgaat gggcaggctg agcagaggct gattcatcgg ggtcatcctc
     1141 ctatttccgt tccggcagcg gcgcaacctc gcgcgcctgc agccgcgccg aagcggcgta
     1201 ctggcgtgct tcggcaggat tgctgcggaa gccctcgaga cgttcgactt cggccaggtt
     1261 gggctcggcg gattcaagct cctccttgct gatgcccttg gcgccggtcg tcgcggcgct
     1321 gccggtcgac ccggttcggg cgacatttcc caactgacct atcccggcca gagagctgcc
     1381 gccgcgcgct gcctcgggac gcaggttgag taggcgaacc caggcggtcc ggccgttgct
     1441 cagcctgacc tggttccagg cgccctgggc gccgatgatc tcgacgcgcg tcttcgtcgc
     1501 cagcattgcg accgtcggcg aatcgacgaa tgggcgctcg tgcgccgtgc tgttgcgaac
     1561 gacggtcgca ggcgttgccg cgacggtcgc cgccagcagc gagagggcta tggcggccag
     1621 cgccggacgc agcagggaca acgatctcat ggcttcctcc ttggttcgaa caagcgcacg
     1681 gcctgcgcgc ggccctttac cgtgaactcg ccgtggtcga tgaattcgaa ggtctggctg
     1741 caggcctcgc aggcgatccg tgtcgcgtcg gaaacgagga cgcgggcaat tcccttggtc
     1801 tggccctcga tgcggctggc gaggttgacc gtatcaccga tcgccgtgta gtcaagccgc
     1861 tggctcgagc cgaggaagcc gacgacggct ggtccgctgt gcacgccgat gccgatgtcg
     1921 aagttctcgc cgagttcgcc cagctcggcc ttgaactcga tcagcttgtc ggccattgcc
     1981 aacgcggcct cgaccgccaa ggccgcgtgc tcggcgcgcg gtagcggcgc gccccagaag
     2041 gccatgatcg cgtcgccgat gaatttgtcg agggtgccgt cgctggcgaa gatcgcctcg
     2101 acctgcaggt cgaaatagcg gttgaggatg cgcacaatct cttcgggcga gcgggtctcg
     2161 gagagggtcg tgaagccgcg gatgtccgag aacagcacgg tgatttcctg gctgcggccg
     2221 gcctgcgcgg cggcgatctc gccttcgttg gtcagcgtgt gcacgacgcg cgggtcgaga
     2281 aagcggccga acatctgcac cgcgtgctcg cggctttgcc gttcctgcag gtgggcgacg
     2341 atggcgatcc cgacaaaggc cagccagacg ccggcgatcg ccgaataggg tcgccattca
     2401 attcccgact gcagcattag ccaggcgctg ccaaccgtga cggcgctggc ggcggcgagc
     2461 gccgctgcag tcatcgccgg gctcaggtgg cgcaggaaag cgagcgtcgt cgtcgcgatc
     2521 agcgccagtg tcagcgcaat gccggcagcc ggcgccagcg gccgcagcgc gtcgccgtcg
     2581 gcgaggttgg cgatggccgt ggcgaggatg tcgggcccgg gcgtcgtcgc cccgagcggg
     2641 gtcggccgca ggtcgtgcag ccccggcgcg ccggcgccga cgatgacgat cttgccgctg
     2701 acgtcggcca gtccgctcgg cgactcgcgg agcgactcga ggtagtagtc ggcataggag
     2761 atgcgcgtga acggtcgcgc ataccagttg aggcggatcg ccgctgccgc cggcagtgcc
     2821 cagccggcgt cggcggcgac gcgtgcggcg attgacgggg tttcccagcc gcgctgcggg
     2881 cggaagagcc agtagcggcg gccgatgccg tcggcgtctt cgaggaagtt gatgatcccg
     2941 gtgcgccaga cgtcgacgtc gagcgccttc ggcgcgatca gcggcagact ggcgtcgagg
     3001 tcggcctgcg gcgtcgcgcg gatgcccatt tgcggcggca ggtcggcgag ccgcgagccg
     3061 gtaccgtcgc cggcgatgac cagcggcagg taagccgggt tctgacgcag catttcactg
     3121 aagaacgcat cgctttccgg gcgaaagacg tcaggttcgc tgaagatcag gtcgaaaacc
     3181 accgccttcg gatggccggc ggcgagcgcc gccagcagct cgccgtggac cgcgcgcggc
     3241 cacggccagt tgccggcaat ctccagcatg cgcgggtcgt cgaggctctt ttggtcgatg
     3301 tcaacgacga cgatgtcggt cggtggcggg cgttgtgcgg cgtgctggcg gagcagccag
     3361 tccccggcgc ggccgtcgag cgcgtagagc cagtcgttgg ccagcgccag caccgcgagc
     3421 agggcggcgg ccgcggcggt gctcatcagg gcgcggcggt cggctgccga acggtgtccg
     3481 cgttgcgccg tcggcgcctg ttctggcggg ggtttgggca tggccgtcga ttatgccacc
     3541 ggcccccggt cttttttctg cacagcctgc agactatagt caggctttgc cgccgcccgg
     3601 acgacaatag atgtcaataa aaatcaacgg tgtggaagtc gcgttcgact cgatcgacga
     3661 ggccgggccg gcggcgccga tcatcacttt cgcccactgc ctgggcggcg accggacgat
     3721 ctggcgcaag caactggcgc actggcgctc ccgctaccgg ctgctgccct atgacatgcg
     3781 tggtcagggc ggcagcacgg cgacgccggg gccttactcg atggaacaac tggccgccga
     3841 cgcgctggcg ctgctcgacg cacagggcgt tgagcggacg atcttctgcg gcgtatcgat
     3901 gggcggcatg gtcgcactgc agctggcgct gctggcgccg cagcgagtcg cggcgctggt
     3961 tctggccgac agtgccggcg gttttggtgc cgaggcgcgc gcggcttgga ccgaacgtct
     4021 ggcagccgtc gaacgcgacg gtgtcgcgcc gttggccgag acgatgatgg ggcgctggtt
     4081 caccgacggc ttccgccagc gcgccgtcgg cgaagtcgcg gaggtcgccg cggcgctagc
     4141 ccgcacgccg gttgccggct acctgggcgc ctgcgccgcg atccgcgatt tcgatctgcg
     4201 cgaacgtttg cgcgaaatcg aatgtccgac gctggtcctg tgcggcgaac acgatccgtc
     4261 gacgccgctc gccctctcgc aggagctggc tgccggcatt cccggcgccc gtctggaggt
     4321 acttgccggc gccaatcacc tgccgaactt tgagcagccc gaacgcttca acgccgtcgt
     4381 cgaccggttt ctggcctcgc tcgggccgga tctggcgctc tgacacccca tatcaacccc
     4441 agcagccggc cgattgcggt cggcgcaacc acggaggaga cctgcatgac ccaggcaatt
     4501 cgaattcaca agaccggcgg tccggatgtg ctgcagtggg aggaggtgaa agttggcgac
     4561 ccggggccgg gcgaggcgcg cgtgcgtcac gaggcggttg gcctcaacta tatcgatacc
     4621 taccatcgga ccgggctcta tccattgccc gcgctgccgt cggggatcgg tcttgagggc
     4681 gccggcgtgg tcgaggcggt cggcagcggc gtcgagggac tgaaggccgg cgatcgcgtt
     4741 gcctatgccg gtggcccacc gggtgcctac agcgaagccc gctgcctgcc ggccgaccgc
     4801 ctgctcaaac tgcccaaggg catcgattcg cgaaccgccg ccggcatgat gctgcagggt
     4861 ctgaccacgg cctatctgct gcgcaagacc tatgccgtgc agcctggcga cgcggtgctc
     4921 attcatgccg ctgccggcgg tgtcggcctg atcgcctgcc agtgggcaaa ggcccttggc
     4981 gcgatggtca tcggtaccgc ctcgaccgag gaaaaggccg aactggcgcg tcagcatggc
     5041 gccgaccacg tgattctcta cagcaaagaa gcgtttgcgc cgcgcgttcg cgaactgacc
     5101 gggggcgaag gcgtggccgt ggtctacgac ggggtcggca aggacacctt tgccggctcg
     5161 ctcgacagcc tgcgggtgcg cggcatgctg gttgcctatg gcaacgcctc ggggccggta
     5221 ccgcccttcg atccgctgct gctgtcgcag aagggttctc tgttcctgac ccggccgacg
     5281 ctcatgcact acactgcaca gcgcgctgaa ctggccgaac tgggcggcga attgttcgat
     5341 gtcgttggcc gcggcgccgt ccgcatagag gttcgccaga cctacgcgct taaggatgcc
     5401 gcgatggcgc atcgggatct cgaaggccgg aagacaaccg gctcgacgat cctgctggtc
     5461 tgagcggcag gcgtatcacg cgggagtccc gactgatcgc agggaggcag aatgcaggta
     5521 accgtcgtcg ccaaccccaa ggggggggtc ggcaagagca cgctggcaac caatctggct
     5581 gggtttttcg cgcggcgtgg cgaatcggtc atgctcggcg atgtcgaccg ccagcaatcg
     5641 gcgcgcgact ggctggcctt gcggccagcc gagttgccgc cgatcgccgg ctgggagatc
     5701 gatcgcaatg cgccggcacg accgcccaag gggacgacgc aggccgtgct cgacacgccg
     5761 gccgggctgc acggcaagct gctggccaag acgctgcgcg tcgccacacg cgtcgtgatt
     5821 ccgctgcagc cttcgctgtt tgacattctc gcgacccgtc atttcctaga cgcactggtc
     5881 gaggagcgcg cctttcgcaa gggtcagctg gcggtcggcg tggtcgcgat gcgcgtcgat
     5941 ccgcgcacgc gcgcggccgc cgagctcgat cgcttcctgg cccagaccga agtgccggtg
     6001 gttgcgcaca ttcgcagcac gcagctctac gtgcagctgg cggcgcgcgg cctgacgctg
     6061 ttcgacgtca atccgaatcc ggtggcgcag gatctggagc agtgggattc gctgattcgc
     6121 tggcttgaca gctgagccag tcgacggcga aggcccgtgc atcgacgaga aaggctctgc
     6181 aatcgtcggc aaagcctgcc gcgtcgccgc cgaggtgaac atcggcgccg gccagcgggt
     6241 tctcccgacg aacacgccgc cccatgcgct gcagcgtcgt ggccagcccg gcggcggtcg
     6301 agtagccgct cagccagtcc tcgcgggcca tcagccggca agcgccctgc gcgccttcgt
     6361 ccatcagatg ccggtaggcg gcgatctcgt gatagagcga ctggctgaac tcggtcagcg
     6421 gctgggggtg ccaatccgcc cattgccggg cgaggatatg gtcgtagtag aggtcgacga
     6481 tgaccccgga ccagcgacgg atattggccg gcagccgggt gcggctggca cggaaggccg
     6541 ggtggctgtc ggcgtagcgg tcgatggccc ggtgcaggcg gatgccccgc gcgaagtcgg
     6601 ctggcagatc gggcgccagc ggacccttga agaaatcgcc ggtcgcgctg ccggcacgca
     6661 gcggcgccgc gaaatcggca agcagggcgt gggcgaggta gttcattggc ggcgtcagtc
     6721 gagcaggcgg tgatgggtcg gacaggtttc ggaggtcaga gtttgcgcga ccgcgtagcg
     6781 cagtggctgc gggcatcgcc ggtttcggta gcgctggccc tcgtgctgct caccgtgtat
     6841 ctggccagcg cattccgcgc tggcgtcgtc tgggagattc cggccgacgc gctgctacgc
     6901 gacggggcca gctacggtcc ggcgctggcg gccggcgagt ggtggcgggt cgtgtcggcg
     6961 gcttttcttc acggaggcgc cctgcacctg ctggtcaacc tcgtcgttct tgccgatgcc
     7021 gcgccattgg tcgaggccgg cggcgcgact gccgaccggc cgcccccgct gctcacgcgt
     7081 ttgcggcggg tgatcggggt tttcatcgtc accgctgccg gcggattcgt cgccagcgcc
     7141 tggtggagtt ccggtgcggt gagcatcggt gcgtcgggtg gcatcctcgg cttctacggt
     7201 tactggctgg cgcgcgtctg gcgcatggcc ggccaaaggg ccgatcagcc caggcggttc
     7261 gggccggtac tggcgcgccg gcacgtcgca ctcgctgccg gctaccttgc cgctgcggtc
     7321 acggcgggct tcctcatccc tggagtcgac aacgcggcgc acatcggcgg cttgctcggc
     7381 ggggcagtgc ttgggtggat gcccaccgcg aacgcgcgcg catcgactgc cgtggcgcag
     7441 ctcgttctcg tcagcgcagc gctgctggct atgcccgcgc aatgggcggc tgactatcgg
     7501 gcgcagcagc atttcaacga aatctacagc cgcttcgtcg agcgcgatcg tcaggccaac
     7561 gccgaaatcc ggcggttgct cggcgagatt cgcgccgggc gtgacccagc ggcggttgcc
     7621 ggcgaattcg atcggctggt ggcgggtcgc ctgcgtgaga accgccagct gtggcttgac
     7681 cagagtgccg atgacgggtc gtcggcactg gcgatcgagc gccagcgctg gcagcgctac
     7741 ggcgatctgc gggtcgatgc ggcggagtcg ctggtcgctg cgctgctggc gccggacggc
     7801 gcgacgcagg cgcgggagct cggccgtttc gagcgcgcga tgcgtgaggc gcgggcgatc
     7861 gccgaggagg caaacagcgc ccttgacaag ccgtgaggcg attgcctcgg gctacaatgg
     7921 cgccaatgca aatgggaagt cagctttact ggggggttcc gtaatgacgc agtaccaatg
     7981 ggatgatcgc ctgctgaccg gtttcgaggt cgtcgattcc caacacaaac gcctgttttc
     8041 actgctgcag gggcttggcg acagcctggc gtcgagcgaa accgatgtcg aaccgctgca
     8101 gagggcggct gatgccctga cgcattacgc gcaggaacat ttcgacgacg aactcgaact
     8161 gatgcagaag cagcgctgcg atctgcggca cgtgatgatg catgtgcagg agcacggtgc
     8221 attcatccgc cgcgtgtcgc tatttcagga gtcgctggcc gagggcaacg ccagcggacg
     8281 tgccaaggcc ggcgaactcg tgcagtttct cgttgagtgg ctcaagcacc acatccgcgt
     8341 cgtcgatcag gtgatggcgc gccagcttcg ccgaatcgcc gatggcgcgt cgccgagcga
     8401 cgcctacacg gccgaagttg ccgaacaccc cgatgcgcgc gccgcccacg ccttcgccga
     8461 ggcgccaaag gactgaagct cgcggcaagg gcgtcgcggg caccggcggg cgagtagccc
     8521 ggcggtagaa tagtcggatg ctcgcgccgc gtttcgttca cctccggctt cactccgaat
     8581 actcgattac cgatggcatg gttcgcctcg atgtgggcaa gaaccatgac tgcccgccgg
     8641 tgcgtcgggc gaccgaggac ggcatgcctg ccgtcgccct caccgacctt gccaacctct
     8701 tcggcatggt caagttctat tcggcggcac gcgcggcggg cgtcaaaccg gttgtcggcg
     8761 ccgacgtctg ggtcaacgat cccgaagcag atcgcccat
//
LOCUS       DATDAA010000219         3576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_296609,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000219 DATDAA010000000
VERSION     DATDAA010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296609"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="VIS73_06975"
     CDS             <1..1202
                     /locus_tag="VIS73_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEY5762933.1"
                     /translation="EWELVKSPAGAHQWQAKDVKQEHMIPDAHDRSQKHRPMMTTADL
                     SLRFDPIYEPISRRFHNNPQAFADAFARAWFKLTHRDMGPRSRYLGPEVPAEVLVWQD
                     PVPVVEQPPIGAGDVAELKAKILATGLSVAELVSTAWASASTFRGSDKRGGANGARIR
                     LAPQKDWEANQPARLSKVLAALEAVQKAFNTVTRKVSLADLIVLGGCAAIEQAAEAAG
                     YAVEVPFTPGRADATQEQTDVESFAVLEPIADGFRNYLKAGVGAPAEELLLDKAQLLT
                     LSAPEMTVLIGGLRVLDANVGQSAYGVFTKRPGTLSNDFFVNLLDMGTAWKPTEADAN
                     RFEGRERATGKPKWTATRVDLVFGSNSQLRALAEVYAQADANEKFVRDFVAVWNKVMS
                     LDRFDLK"
     gene            1364..1741
                     /locus_tag="VIS73_06980"
     CDS             1364..1741
                     /locus_tag="VIS73_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762934.1"
                     /translation="MKAPNPNIALHAGRIIVAVLLLAQTACSQLTLPPGDGTSLAERR
                     RMLDEDPGAGITVIPRRPAEEAAASATADGSNATDSQDPGELSPPSGRSMSDAKKLSS
                     PDLGIRIPIGQPALTPRQYSPQE"
     gene            complement(1895..3226)
                     /locus_tag="VIS73_06985"
     CDS             complement(1895..3226)
                     /locus_tag="VIS73_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762935.1"
                     /translation="MRTTMSILVSIIVIAATACGGGGNGGATAHAPVIANLSFSPTTI
                     YQSQDGTASITGDFSFSDAGGDVVAVRMTTSAGADLTLPVSAPGITSGTLAGNLTISA
                     GDIGHYAFEVWVTDSTGAASNRLSGTVDVIADDRAIQWTMLSVAPPRILYGIAKAGDQ
                     VVAVGAGGTVMSSSDLVNWATPSSGVAHLLRSVAWSGSRFVAVGDDSATGEAVILSSD
                     DAANWTVRKTAGECTATGCAAPAILSSVIWTGTQFVAVGQTVLSPGGPHALVLTSADG
                     ATWLQQAAGTVVLGTASESAFQSVVWSGSQFVAVGYGPDGNAAAWTSADGDTWTWRTI
                     QGTPNQVLRDLTWAGGRYVAVGWSGASGTMFRSTDGVTWEGNADIPVLPAMNTVTGGP
                     SRFVAMSNVYVATSSDGQTWSLEGATAACGNDVIWDGIRYLSVGISICRSP"
BASE COUNT          667 a         1124 c         1147 g          638 t
ORIGIN      
        1 acgagtggga gctggtcaag agtccggccg gcgctcatca gtggcaggcc aaggacgtga
       61 aacaggaaca catgattccg gacgcccatg accgatcgca gaagcaccgg ccgatgatga
      121 ccacggccga cctgtcgctg cgcttcgatc cgatctacga gccgatctcg cggcgcttcc
      181 acaataaccc gcaggccttt gccgatgcct tcgcccgcgc ctggttcaag ctgacccacc
      241 gcgacatggg cccgcgctcg cgctacctag gtcccgaggt gcccgccgaa gtgctggtct
      301 ggcaggaccc tgttcccgtc gtcgagcagc caccgatcgg cgccggggac gttgccgaat
      361 tgaaggccaa gattctcgcc acgggtcttt cggtcgccga gctggtgtcg actgcctggg
      421 catcggcttc caccttccgc ggctcggaca agcgcggtgg cgccaacggc gcacgtatcc
      481 gcctcgcccc gcagaaggac tgggaggcca accagccagc gcggctgagc aaggtgctcg
      541 ctgcccttga agcggtgcag aaggccttca atactgtcac caggaaggtc tcgctcgccg
      601 atctgattgt gctcggtggt tgtgcggcca ttgagcaggc ggccgaggca gccgggtacg
      661 cggtcgaggt gccgttcacg ccgggacgtg ccgatgccac gcaggagcag accgatgtcg
      721 agtcctttgc cgtgctcgaa ccgattgccg atggcttccg caactacctc aaagcaggcg
      781 tcggtgcgcc ggccgaagaa ctgctgctcg acaaggcgca gttgctgacc ctgagcgcgc
      841 cggagatgac ggtgctgatc ggcggcctgc gtgtgctcga tgccaatgtc gggcagtcgg
      901 cttatggtgt tttcaccaag cggccaggca cgctgagcaa cgacttcttc gtcaatttgc
      961 tcgacatggg caccgcgtgg aagccgactg aggcagatgc caatcgcttc gagggcaggg
     1021 agcgtgcgac cggcaaaccg aagtggactg ccacgcgcgt cgacctcgta ttcggctcca
     1081 actcgcagct gagggcactg gccgaggtct acgcgcaggc cgatgcaaac gagaagttcg
     1141 tccgcgactt tgtcgccgtc tggaacaagg tgatgagcct cgatcgcttc gacctgaagt
     1201 gatccggcta gggttgccgc gacgtggcag cacgcgtgtc gcggcaaccc ctatgctgct
     1261 cgatcgggcg ggctaaaggg tgccagcccg aatacgccaa ccgattcaaa cgcgatcgtg
     1321 gccgattgat cccgcgacat ccgggcgcgg taacatcgcg gccatgaaag cgcccaaccc
     1381 caacatcgcc ctacacgccg gccgcatcat cgtggccgtg ttgctgctcg cacagacggc
     1441 gtgcagccaa ctgacgctgc cgcccggcga cggcacctcg cttgcggaac gccggcgcat
     1501 gctcgacgaa gaccctggcg ctggcatcac cgttatcccc aggaggcccg ccgaggaagc
     1561 cgcggcctcc gcgaccgccg atggcagcaa cgcgacggat tctcaggatc ccggagaact
     1621 gtcgccacca agcggccgct cgatgagtga cgccaagaaa ctgtcgtcgc ccgatctggg
     1681 aatccgaatc ccgatcgggc aaccagcgtt gacgccgcgc cagtactcgc cgcaggagta
     1741 gggcggctga agggcagttg ttcggacatt ccggccggtc atgccgagca aattgagcga
     1801 cagctgtcag cttggttgct gaccgtcaga ctcagcgact gctttcgggt ggcctgagaa
     1861 aaaccgggca ggtcagtcgg cgcgacgtgc tggttcaagg cgagcgacag atcgaaattc
     1921 caaccgacag gtagcggatt ccgtcccaga taacgtcatt gccgcaggcc gccgtcgcgc
     1981 cttcgagaga ccacgtttgc ccatcggacg atgtggcgac gtagacgttg ctcatggcaa
     2041 cgaatcgcga cgggccgcca gtcactgtat tcattgcagg cagcaccggg atgtcggcat
     2101 tcccttccca agtcacgccg tcggttgatc gaaacattgt gcccgaagcg ccactccagc
     2161 cgaccgcgac gtagcgcccg ccagcccagg tgaggtcgcg caatacctgg tttggtgttc
     2221 cttgtattgt gcgccaagtc catgtatcgc catccgctga tgtccatgcc gccgcattgc
     2281 catctggccc ataaccgacc gcgacaaact ggcttccgga ccagactacc gactggaaag
     2341 cgctttcaga agcggtgcct agcacaacgg ttccggcggc ttgctggagc caggtcgcgc
     2401 cgtcggccga ggtaaggacg agggcatggg gaccgccggg gctcagtacg gtttggccga
     2461 cggcgacgaa ctgtgtgccg gtccagatca cgctggagag gatcgcgggt gcggcacaac
     2521 cggtagcggt gcattcgccg gctgtcttcc ggactgtcca gtttgccgca tcgtcggagg
     2581 agaggatcac cgcttcgccg gtcgcgctgt cgtctccgac ggcaacaaag cgcgatccgg
     2641 accaagcgac gcttcgcagc aggtgcgcga cgccgctgga gggagtggcc caattcacga
     2701 gatcgctgct gctcataacc gtgccgccag cgccgacggc gactacctga tcgcctgctt
     2761 tggcaattcc gtagaggatt cgcggcgggg ccacggaaag catagtccac tgaattgccc
     2821 ggtcgtccgc gatcacgtcg acggtgccgg acaaccggtt ggatgcggcg ccagtactgt
     2881 cggtgaccca gacctcgaag gcgtaatggc cgatatcccc ggccgagatg gtgaggttgc
     2941 cggccagcgt tccgctggtt atgcctggcg cactgaccgg caaggtcagg tcggcgccag
     3001 cgctggtggt cattcgtacc gcgacgacgt caccgccagc atctgagaag ctgaaatcgc
     3061 cggtaatcga tgcggttccg tcctgcgatt ggtagatggt ggtgggcgag aagctgaggt
     3121 tggcaatcac cggcgcgtgt gcagtggcgc cgccgttgcc accgccaccg caggccgttg
     3181 ccgcaataac aatgattgag accaagatcg acatcgtcgt gcgcatggcc gtctccttgg
     3241 cgaggatcta agggagtcct ctatttgatc gtaggagacg ccgatcttct tggacttgac
     3301 ttcggtcaag cgaaacaagc cgccggaagg aatatcgggg agggttcagg gtgttcgttg
     3361 tgatgggttt tgattgcctg gccgctgctg gggtgaagcc gtcctccgaa ggtccgcttt
     3421 cacggatcgg cgaacgaccg gttgaatccg caacccggaa gagacattcg ccgtttctgc
     3481 gatccaacgg cgcgtttctg agcgaagcag tcgtaccgag cagcgtctgt ctaatttcta
     3541 ctcggccact ccggccgctc gtttcaccca gaacta
//
LOCUS       DATDAA010000220         6082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_156038,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000220 DATDAA010000000
VERSION     DATDAA010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156038"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VIS73_06990"
     CDS             complement(<1..563)
                     /locus_tag="VIS73_06990"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEY5762936.1"
                     /translation="MKAEAGGLGWRLSLFLVVYRAVWYLLAPGVLAYFWRRGKREPVY
                     RQFFDERFGGGGLPAGWNRDCRPVWVHCASLGEFRGAAPLIRAMLDSGLPILFTTVTP
                     AGRGAAQSLFAAEIEARRMAIAWSPLEFAWAVRRLVARYRPHCAMMCEIDTWPVLVTT
                     IRQAGVPLAMANAQYPGKSLVRDERWGG"
     gene            complement(553..1674)
                     /gene="neuC"
                     /locus_tag="VIS73_06995"
     CDS             complement(553..1674)
                     /gene="neuC"
                     /locus_tag="VIS73_06995"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEY5762937.1"
                     /translation="MSSRQVLFVTGTRADYGKLEPLAEAARDGGFGISFFITGMHMMA
                     RYGETRHEVKRFAGAECFEFVNQRAGDPQDLVLAKTVLGFSDFVHEHRPDLVVVHGDR
                     IESLAVSLVCATRYLPAAHIEGGEVSGNIDESIRHCNSKLCASHFVSSEQARNRVLSL
                     GEPDSSIYVIGSPELDSHGRPSGVAMDDVRRRYDIPFIDYGIAVFHPVTSEVDTIGAQ
                     AQALFDRCEASGRNFVVILPNNDPGTEDVMAVIERLPEARFRLIPSMRFAYFSELMRN
                     ASAFVGNSSVGVREAPFLGIPSLDVGTRQSGRAAAPSVTGCSPYDAAAIDAFLARSWG
                     QRLPPDTSFGAGNAAQRFVDILRDEAFWNRSLQKTFHES"
     gene            complement(1817..2866)
                     /locus_tag="VIS73_07000"
     CDS             complement(1817..2866)
                     /locus_tag="VIS73_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018401706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEY5762938.1"
                     /translation="MKIGNREIGPAHPPLVIAEIGINHGGDLTVAKGMVLAAQRAGCE
                     MVKHQTHFVDDEMTEEAKQIFPPNADVSIWEVMARCALSKDDEIALKAYAESLGMIYI
                     STPFSRAAADFLNEIGVPGYKIGSGECDHVPLIRHIARFGKPIIMSTGMQSIDSLRAS
                     VAVLDEAGVDYALLQCTNLYPSPPEIVSLGGINELRAAFPRAVIGFSDHSIGPSMALA
                     SVALGACILERHFTDTRYREGPDISCSMDPAELRFLVDRSREIHTALRNPKRRTGPEE
                     AVYRFARGSVVAERELPAGHVIGEADIWARRPGSGEIAVAEFDRVIGARTRRALKRNQ
                     QLKWDDLELASGEKR"
     gene            complement(2863..3552)
                     /locus_tag="VIS73_07005"
     CDS             complement(2863..3552)
                     /locus_tag="VIS73_07005"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEY5762939.1"
                     /translation="MASTWTAVVPVRRGSKGLPGKNLRPLAGKPLYRHAVDLALAAGA
                     TRVIVSTDIPEILAAPETAGVSVLERPAELCADETPMAPVLKHALDALAVSGPVVLLQ
                     ATSPLRRIEQLREALARFAAGGVELVMSVTAANRSVLKWGFVNDGHFTPIGKPEWCFA
                     NRQQLPAVYRPNGAIYVVDSARFRREGSFVTPQVGVVVMPEEDSGDIDSLADFEHCAA
                     VLAARNEEEKR"
     gene            complement(3652..>6082)
                     /locus_tag="VIS73_07010"
     CDS             complement(3652..>6082)
                     /locus_tag="VIS73_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5762940.1"
                     /translation="DPEYLATRTVAPAANPRQQVPIESVALVQSDRARAGTGTLHEGR
                     RAVLLAITKKSYVNTLDLVASIDTFVASRGPVLAQSGIEAKLLDDQTIPTREAIGVME
                     RNALQGLVLVLLICWVFLGTRMALLVGLGIPFALAGTFAILNAAGFTLNISVLLGVVI
                     ALGMIVDDVVVVIEAIYYRLQRGADVVSASLDSLREVFAPVTASVATTLAAFLPLMLL
                     PGIVGKFMFVIPFVVTLALLVSLLEAYWMMPSHVISLNPDFDKPTRMSAWRWRFLHGL
                     RLNYSRWLLAAFRRPLLTATIIVTLVIGGVAAVASGAVRVQFFAFDPLRIFYVNVDMP
                     AGTPLEATLAKTTELEARVRRHIEPGEARGIASYAGVKFTDTEPLYGESYGQVIVSLN
                     PRSDSVREVEDVVASLRADAESLPPPGRVNFTLLSGGPPLAKPVKVRVRGDDVVELRA
                     AADALTEVVRGIPGTRDIADDDVPGRPELTLRVDRDAVRAAGVSPAFVARAIRLHSDG
                     EVVAVMRDRGEKVDVRVRAQYEGPDGRSIGPDIARLLAQPVALPGGGVTTIGALAQAE
                     TAIGRGVIKHYNLRRAITVEADLDKEITDTVVANRLIAEAWEEVAARFPATDIDFSGE
                     LDDIQESLDAMAQLFLLGVGLIYLILATQFRSYWQPFLILVTVPLAFTGVAVGLLVSR
                     NPISLYTLYGVIALAGIAVNSAIVLIDATNTRRAAGMSVLHAAVYAARRRVVPILMTS
                     ATTIGGLISLALGLGGKSLLWGPVASAIVWGLAVSTAMTLFTVPLLYWASMRGGGWAP
                     GLRRRMGLSRR"
BASE COUNT         1016 a         2037 c         2118 g          911 t
ORIGIN      
        1 ccgccccagc gctcgtcgcg cacgaggctc ttgcccgggt actgggcgtt ggccatcgcc
       61 agcggcacgc cggcctggcg gatcgtcgtc accagcaccg gccaggtgtc aatctcgcac
      121 atcattgcgc agtgcggccg gtacctcgcg accagacggc gcacggccca ggcgaactcg
      181 agtggcgacc aggcgatcgc catccgccgc gcctcgattt cggcggcgaa cagcgactgc
      241 gcggcgcctc gtccggccgg cgtcacggtc gtgaagagaa tcggcaggcc gctgtcgagc
      301 atggcgcgga tcagcggcgc ggcgccgcgg aactcgccga gcgaggcgca gtgcacccag
      361 accggccggc aatcgcggtt ccatccagcc ggcaacccgc cgccgccgaa gcgctcgtcg
      421 aagaactgcc ggtagaccgg ctcgcgcttg ccgcgccgcc agaaataggc gagcacgccg
      481 ggcgccagca ggtaccagac ggcccggtag acaaccagga acagcgacag ccgccagccg
      541 agcccgccgg cctcagcttt catgaaaggt tttctgcagc gagcggttcc agaatgcctc
      601 gtcgcgcagg atgtcgacga aacgctgcgc ggcgttgccg gcgccgaagc tggtgtccgg
      661 cggcaggcgc tggccccagc tgcgcgcgag gaaggcatcg atcgccgccg cgtcataggg
      721 cgagcaaccg gtgaccgagg gcgccgcggc gcggccgctc tggcgggtgc cgacgtcgag
      781 gctgggaatg ccgaggaagg gcgcctcgcg cacgccgacg ctgctgttgc cgacgaaggc
      841 gctggcgttg cgcatcagct cggagaagta ggcgaagcgc atcgacggaa tcaggcggaa
      901 gcgcgcttcg ggcaatcgct cgatgaccgc catcacgtcc tcggtgcccg gatcgttgtt
      961 gggcaggatg acgacaaagt tacggccgct cgcttcgcag cggtcgaaca gcgcctgcgc
     1021 ctgcgcgccg atggtgtcga cctcggaggt gaccgggtgg aagacggcga tcccgtagtc
     1081 gatgaagggg atgtcgtagc gccggcgtac gtcgtccatc gccacgcccg atgggcggcc
     1141 gtggctgtcg agttcgggcg agccgatcac gtagatgctc gaatcgggtt cgccgagcga
     1201 gagcactcgg ttgcgcgcct gctcggagct gacgaaatgg ctggcgcaga gcttggagtt
     1261 gcagtggcgg atgctctcat caatgttgcc cgaaacctcg ccgccctcaa tgtgcgcggc
     1321 cggcaggtag cgcgtcgcgc agaccagcga caccgccagc gattcgatgc ggtcgccgtg
     1381 gacgacgacg agatcgggcc gatgctcgtg gacgaaatcg gagaagccga gcacggtctt
     1441 cgccagcacg aggtcctgcg gatcgccggc gcgctggttg acgaactcga aacactcggc
     1501 gccagcaaaa cgcttcacct cgtgccgcgt ctcgccgtag cgcgccatca tgtgcatgcc
     1561 ggtaatgaag aacgagatgc cgaaaccgcc gtcgcgcgcg gcctcggcca gcggctcgag
     1621 cttgccgtag tcggcgcgcg tgccggtgac gaaaagaacc tggcggctgc tcattgcgcg
     1681 cctcggtcgc cgacgcgctc cgcgcacgag cggggtttgt gggcccgccc cccgtccccc
     1741 tggccgtgtg cggggacggc ggtggcgcgc cgcggcgcga cccgcggatg tccgtgcatg
     1801 aatccggtgg cgccgctcat cgcttctcgc ccgacgccag ctcgaggtcg tcccatttca
     1861 actgctggtt gcgcttaagt gcgcgcctgg tgcgggcgcc gatgacgcgg tcgaactcgg
     1921 cgacggcgat ctcgcccgat cccggacgtc gggcccagat atcggcttcg ccgatcacgt
     1981 ggccggccgg cagttcgcgt tcggcgacca ccgagcctcg cgcgaaacgg tagacggctt
     2041 cctcgggccc ggtgcgccgt ttgggattgc gcagcgccgt gtggatctct cggctgcggt
     2101 cgaccaggaa gcgcagctca gccgggtcca tcgagcagga aatgtccggc ccttcccggt
     2161 agcgcgtgtc ggtgaaatgg cgctcgagga tgcaggcgcc gagcgccacc gaggcgagcg
     2221 ccatcgacgg gccgatgctg tggtcggaga agccgatcac ggcgcgcgga aaggcggcgc
     2281 gcaattcgtt gatgccgccg aggctgacaa tctcgggcgg cgagggatag agattggtgc
     2341 actggagcag cgcgtaatcg acgccagcct cgtcgagcac ggccaccgag gcgcgcaggc
     2401 tgtcgatcga ctgcatgccg gtgctcatga tgatcggctt gccgaaacgg gcgatgtggc
     2461 ggatcagcgg tacgtggtcg cattcaccgg agccgatctt gtagccgggc acgccgatct
     2521 cgttgaggaa atccgccgcc gcgcgcgaga acggcgtcga gatgtaaatc atccccagcg
     2581 attcggcgta agccttgagc gcgatctcat cgtccttcga cagcgcgcag cgcgccatca
     2641 cctcccagat cgacacatcg gcattgggcg ggaaaatctg cttcgcctcc tcggtcatct
     2701 cgtcgtcgac gaaatgcgtc tggtgcttga ccatctcgca gccggcgcgc tgcgccgcga
     2761 gcaccatgcc cttggcgacc gtgaggtcgc cgccgtgatt gatgccgatc tcggcgatca
     2821 ccagcggcgg atgcgccggt ccgatttccc tgttgccgat cttcatcgct tctcttcctc
     2881 gttgcgcgcg gcgagcacgg ccgcgcagtg ttcgaaatca gccaggctgt cgatgtcgcc
     2941 gctgtcctcc tcgggcatca cgacgacgcc gacctgcggc gtgacgaaac tgccttcacg
     3001 gcgaaagcgt gccgaatcga cgacgtagat cgcgccgttg gggcggtaga ccgcgggcag
     3061 ctgctggcgg ttggcaaagc accactcggg cttgccgatc ggcgtgaaat ggccatcgtt
     3121 gacaaatccc cacttgagca cgctgcggtt ggcagcggtc accgacatca ccagttcgac
     3181 gccgccggcg gcaaagcgcg cgagcgcctc gcgcagctgt tcgatacggc gcagcggcga
     3241 ggtcgcctgc agcagcacga ccgggcccga gacggccagc gcatcgagcg cgtgcttgag
     3301 cactggcgcc atcggcgtct cgtcggcaca gagttcagcc ggtcgttcga gcacgctgac
     3361 cccggcggtc tccggcgccg cgaggatttc cgggatgtcg gtgctcacga tcacgcgtgt
     3421 cgcgccggca gccagcgcca gatcgaccgc atgccgatag agcggcttgc cggcgagcgg
     3481 ccgcaggttc ttgccgggca gccccttcga gccgcgccgc accggcacta ccgcagtcca
     3541 ggttgacgcc aatcgctttc ctcgctgccg gctacccggc cccacgcccg gcttaagccg
     3601 gcgaagcggg attataggtt gccgaaccgg cttcgccgaa tcggcaaggc ctcatcgccg
     3661 gctcaggccc atgcggcggc gcaggcccgg cgcccagccg ccgccgcgca tgctcgccca
     3721 gtaaagcagc ggcacggtaa acagcgtcat cgccgtcgac accgccaggc cccagacgat
     3781 tgccgaggcc accggccccc agagcagcga cttgccgccg agcccgagag ccagcgaaat
     3841 cagcccgccg attgtagtgg cgctggtcat caggatcggc acgacgcggc ggcgtgccgc
     3901 gtagaccgcg gcgtgcagca cgctcatgcc ggcggcgcgg cgggtgttgg tggcgtcgat
     3961 cagcacgatg gccgagttca ccgcgatacc ggcgagggcg atgacgccat agagcgtgta
     4021 cagcgaaatc gggttgcgcg agacgagcag gccgacggcg acgccggtga aggccagcgg
     4081 cacggtgacg agaatcagga agggttgcca gtagctgcgg aactgggtcg caaggatcag
     4141 gtagatcaac ccgacgccga gcaggaagag ctgcgccatc gcgtcgaggc tttcctgaat
     4201 gtcatcgagt tcgcccgaga agtcgatgtc ggtcgccggg aaacgcgcgg cgacctcttc
     4261 ccaggcctcg gcgatcagcc gattggcgac gacggtgtcg gtgatttcct tgtcgagatc
     4321 ggcctcgacg gtgatcgccc tacgcaggtt gtagtgcttg atgacgccgc gtccgatcgc
     4381 ggtctccgcc tgcgccagcg cgccgatggt ggtaacgccg ccgccgggca gggcgaccgg
     4441 ctgagcaagc aggcgagcga tgtcggggcc gatgctgcga ccgtcggggc cctcgtactg
     4501 ggcgcgcacc cgtacgtcga ccttttcgcc gcggtcgcgc atcaccgcca ccacctctcc
     4561 gtcgctgtgc aggcggatcg cccgcgctac aaaggccggg ctgacgccgg ccgctcgcac
     4621 tgcatcacgg tcgacgcgca gcgtcaactc tgggcggccg ggcacgtcat cgtcggcaat
     4681 atcgcgcgta cccgggatac cgcgaacgac ttcagtcaac gcgtcggcgg cggcgcgcag
     4741 ctcaacgacg tcgtcgccgc gcacgcgcac cttgaccggt ttggccagcg gcgggccgcc
     4801 cgagagcagt gtgaagttga cgcgccccgg tggcgggagc gactcggcgt cggcacgcag
     4861 agaggccacc acgtcctcga cctcgcgcac gctgtcgctg cgcgggttta gcgagacgat
     4921 cacctgcccg taggactcgc cgtagagcgg ctcggtatcg gtgaacttga cgcccgcgta
     4981 gctggcgatg ccgcgtgcct cgccgggctc gatgtggcga cgcacgcgcg cttcgagctc
     5041 ggtcgtcttt gccagcgtcg cctcgagcgg cgtgcctgcc ggcatgtcga cgttcacgta
     5101 gaagatgcgc aacgggtcaa aggcgaagaa ctgcacgcgc acggcgccgc tggcgaccgc
     5161 ggcgacgccg ccgatgacca gcgtgacgat gatcgtcgcc gtcaagagcg gcctgcgaaa
     5221 ggcggcgagc agccagcgcg agtagttcag ccgcagcccg tgcaggaagc gccagcgcca
     5281 ggcgctcatg cgcgttggct tgtcgaagtc ggggttcagc gagatgacgt gcgagggcat
     5341 catccagtag gcctcgagca ggctgaccaa gagcgccagc gtgacgacga agggaatcac
     5401 gaacatgaac ttgccgacga tgcccggcag cagcatcagc ggcaggaacg cagcaagcgt
     5461 cgtcgccacc gaggcggtca ccggcgcgaa cacctcgcgc aggctgtcga gcgaggcgct
     5521 cacgacatcg gcaccgcgct gcaaacggta gtagattgcc tcgatgacta ccaccacgtc
     5581 atcgacgatc atgcctagcg cgatgaccac gccgagcagc accgagatgt tgagcgtgaa
     5641 gccggcggcg ttgaggatgg cgaaagtgcc ggccagcgcg aagggaatcc cgagccccac
     5701 taggagcgcc atgcgcgttc cgaggaaaac ccagcagatc aacagcacca gcaccagccc
     5761 ctgcagcgcg ttgcgctcca tgacgccgat ggcctcgcgc gtcggaatcg tctggtcgtc
     5821 gagcagcttg gcctcgatgc cgctctgggc gagaaccgga ccacggctgg cgacgaaggt
     5881 gtcgatgctg gcgacgaggt cgagcgtgtt gacgtagctt ttcttggtga tcgccagcag
     5941 cacggcgcgt cgcccttcgt gcagcgtgcc ggtgccggcg cgggcgcggt cgctctgcac
     6001 cagcgccaca ctctcgatcg gcacctgttg ccgcgggttg gcagctggcg cgacggtgcg
     6061 cgtcgccaga tactcggggt cg
//
LOCUS       DATDAA010000221         3230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_401038,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000221 DATDAA010000000
VERSION     DATDAA010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401038"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VIS73_07015"
     CDS             complement(<1..569)
                     /locus_tag="VIS73_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762941.1"
                     /translation="MSDLLAGPALDPETLEERLEVFLFAAPSHRSATRIANGLAGISR
                     RQQEFALRWAEIAAQTNTELGYLVAMLAPQLLARLDTDGSEALILAALERYDQSGGRA
                     AMEMLRDIEGFRLRRERGDAVARLESVESRLERLLQGLSGRRLRIEEAGDGRAWTDTE
                     VVYLPSAIADADKHADNIAHYRVRAAILWG"
     gene            complement(586..1383)
                     /locus_tag="VIS73_07020"
     CDS             complement(586..1383)
                     /locus_tag="VIS73_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEY5762942.1"
                     /translation="MTPTLPHIGEQPYYRPQGEEVELFSAAKTRRLPVLLKGPTGCGK
                     TRFVEYMAWQLGRPLVTVACHDDLSTADLIGRYLVIDDNTVWVDGPLTAAVRAGAICY
                     LDEIVEARKDTTVVIHPLADSRRILPVEKRGEFIQAPDDFMLVVSYNPGYQSVLKEMK
                     PSTRQRFVAIEFDYPDAATETTIVASEGGVDDDLARRLVKLGELTRNLKGAGLDEGAS
                     TRLLVHAAQLIADGVAPRIACSGAIAQPLSDDPEMLASLGDLVHSVF"
     gene            complement(1526..1855)
                     /locus_tag="VIS73_07025"
     CDS             complement(1526..1855)
                     /locus_tag="VIS73_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase-stabilizing/protective protein NifW"
                     /protein_id="HEY5762943.1"
                     /translation="MSETENKLQAELADLSSAEDFFDYFALPYDRAILNVSRLHILKR
                     FNQYIAKAGGIDSFAPREAYGRCREYLAQAYRDFLTSSGIEEKIFRVFQQAQGVQTVS
                     LDTLRRA"
     gene            2183..2506
                     /gene="iscA"
                     /locus_tag="VIS73_07030"
     CDS             2183..2506
                     /gene="iscA"
                     /locus_tag="VIS73_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEY5762944.1"
                     /translation="MSITLTDRAAEHVSRYLTKRGRGIGLRVGVQTSGCSGMAYRLEY
                     ADLTRDEDRIFESKGVRLVVDPTSLPYVDGTELDFVREGLSEGFKFNNPNAADTCGCG
                     QSFKV"
     gene            2634..>3230
                     /locus_tag="VIS73_07035"
     CDS             2634..>3230
                     /locus_tag="VIS73_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY5762945.1"
                     /translation="METIKMDAKRRSASDLEVLALYEISKLLTSSLDFRKCVRQVLNL
                     MTSELDMRHALLGMVQDDGKLHIIGASDVPWQRDKAPILDANIGLLAKVLKNGSPIVV
                     PDIAQDPRFLNRTGRNIEKSEGVAYIAVPLRLDREIIGVLSVDRHPGSGRPINFERDV
                     RLVTLVANLLAQTYQLHLTVAAERLELMAETHRLQKQLQ"
BASE COUNT          571 a          994 c         1054 g          611 t
ORIGIN      
        1 ccccagagga tggcggcgcg cacacggtaa tgtgcgatgt tgtcggcatg cttgtcggca
       61 tcggcgatcg cgcttggcag atagaccacc tcggtgtcgg tccaggcgcg cccatcgccg
      121 gcctcttcga tccgcaggcg gcgacccgac aacccctgca gcagccgttc gagccgcgat
      181 tcgaccgatt cgaggcgagc cacggcgtcg ccgcgttcgc ggcggagccg gaagccctct
      241 atgtcgcgca gcatctccat cgcggcgcgg ccgccggact ggtcatagcg ttcgagcgcg
      301 gcgagtatca gcgcctcgct gccgtcggta tctaggcgcg ccagcagctg cggcgccagc
      361 atcgccacca gatagccgag ttcggtgttg gtttgcgccg cgatctcggc ccagcgcaac
      421 gcgaattcct gctgccggcg cgagatgccc gccagtccgt tggcgattcg cgtcgccgag
      481 cgatgcgagg gcgcggcgaa gagaaagact tcgagccgtt cttcgagcgt ttccggatcg
      541 agtgccggtc cggcgagcag atcgctcatt cccgcaatct ggcggctaga acacgctgtg
      601 aacgaggtcg ccgagcgagg cgagcatttc cggatcgtcc gacagcggtt gggcgatcgc
      661 accggaacag gcgattcgcg gcgcgacgcc gtcggcgatc agctgcgccg cgtgcaccag
      721 cagccgcgtg ctggcgcctt cgtcgaggcc ggcgcccttt aggttgcgtg tgagttcgcc
      781 gagcttgacg aggcgccgcg cgaggtcatc gtcgacgccg ccttcgctgg cgacgatggt
      841 cgtctccgtc gccgcatcgg gatagtcgaa ctcgatggcg acaaagcgtt gccgggtcga
      901 cggcttcatt tccttgagca cgctctggta gcccgggttg taggaaacga ccagcatgaa
      961 gtcgtcgggt gcctggatga actcgccgcg cttttccacg ggcaggatgc ggcgcgaatc
     1021 ggcgaggggg tggattacca ctgtggtgtc cttgcgtgcc tcgacgatct cgtcgaggta
     1081 gcagatcgct ccggcgcgca cggccgcggt cagcggcccg tcgacccaga ccgtgttgtc
     1141 gtcgatgacc agataccggc cgataaggtc cgcggtggaa aggtcgtcgt ggcatgccac
     1201 ggtaaccagt ggccggccga gttgccacgc catgtactcg acgaagcggg tcttgccaca
     1261 gccggtcggg cccttcagca gtaccggcag gcgccgcgtc ttggccgccg aaaacagttc
     1321 gacctcttcg ccctgcggac gatagtaggg ttgttcgcca atgtgcggca gcgttggcgt
     1381 gatgacgccg atggcggatt cgggaagcgt ggcggtcacg atgctcgagg gctccaggcg
     1441 gattgcgcgt gcgatacgcg tcgtccgttc atgaccggtc gccgcggggg gagttccccg
     1501 cgaacgactg gctcgccggc gccgcttagg cgcgtcgcag cgtatcgagg ctgacggtct
     1561 gcacgccctg cgcctgctgg aagacgcgga agatcttttc ttcgatgccg gagctcgtca
     1621 ggaagtcgcg ataggcttgc gcaagatatt cgcgacagcg gccataggcc tcgcgcggcg
     1681 caaacgaatc gatcccgccg gctttggcga tgtactggtt gaagcgcttg aggatgtgca
     1741 ggcggctgac gttgagaatc gcgcggtcgt agggaagcgc aaagtagtcg aagaaatcct
     1801 cggcgctcga caggtcggcc agttcggctt gcagcttgtt ttcggtttcg ctcatggtgt
     1861 tctcctgggt ggattaccgc gtccttgttc tgaatcgttc accgtcaagg cacgcgagat
     1921 acccaacgca acttcgatgc cagtcgttgc agaccggctc gactgatcgc tggtccgtga
     1981 ttcggcgcgg ttgcgacaaa gcgctgtcgt acccccgtga tttttctgtc gcaaatgcaa
     2041 cagaccgatc gcgggcgccc cggcgcagca cgcatcgaat cctccgaaat gaccatcgca
     2101 gcgtttgttt ctggcaatcg ctcggctgct ggcaggcaac ttgcagaact ggccttgtca
     2161 tcaaccgcca aggagaaacg cgatgtcgat aaccctgacc gatcgtgccg ccgaacatgt
     2221 cagccgctat ctcaccaagc gtggccgcgg tatcggcctg cgcgtcggtg tgcagaccag
     2281 cggctgctcg ggaatggcct atcgcctcga atacgccgac ctcacgcgcg acgaggaccg
     2341 catcttcgaa tcgaagggcg tgcgcctggt tgtcgacccg acgagcctgc cctacgtcga
     2401 cggcacggaa ctcgattttg tgcgcgaagg actgagcgaa ggttttaagt tcaacaaccc
     2461 gaacgccgcg gatacctgcg gctgtggaca gagctttaag gtctgaatcg cactttgtat
     2521 gcggcggtat cgcgtcggcg attcattctg cagtgcattc aaggggcacg ctttgtgcat
     2581 cgtttatcgc gttgccccca ggagcgtggc tcgacgaagg gccgcggtta acgatggaaa
     2641 caatcaagat ggatgcgaaa cggcgcagcg cttccgatct ggaagtgctc gcgctctacg
     2701 aaatcagcaa gttgctgacc tcttcgctgg acttccgcaa atgcgtgcgg caggtgctga
     2761 acctgatgac cagcgaactc gacatgcgcc acgccttgct cggcatggtg caagacgacg
     2821 gcaagctgca catcatcggt gcgtccgacg taccgtggca acgcgataaa gcgcccatac
     2881 tcgacgcgaa catcggcctg ttggcgaagg tcctgaagaa cggatcgccg atcgtcgttc
     2941 ccgatattgc gcaggacccg cgattcctca accgaaccgg gcgcaatatc gagaagagcg
     3001 aaggggtggc ctatattgcc gtgccgctcc gcctcgaccg cgagatcatc ggcgtgctct
     3061 cggttgaccg tcaccccggg agcggtcggc cgatcaactt cgaacgcgac gttcgtctcg
     3121 tgacgttggt tgccaacctg ctggcgcaga cctaccagct gcatttgacg gtcgccgccg
     3181 aacgacttga gctgatggcg gagacccatc gcctgcagaa acagttgcag
//
LOCUS       DATDAA010000222         2373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_226337,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000222 DATDAA010000000
VERSION     DATDAA010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226337"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VIS73_07040"
     CDS             <1..290
                     /locus_tag="VIS73_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762946.1"
                     /translation="IAVPKSFAVKFPTEFMFPWRPWLYVRALLPWVRRRREQLVAVTL
                     NGMSIKPEKLIALEDGAIVVDEKGRSMVWSERTMGANKAKIAAFYAGVVGQ"
     gene            287..1057
                     /locus_tag="VIS73_07045"
     CDS             287..1057
                     /locus_tag="VIS73_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEY5762947.1"
                     /translation="MKLPGLAVYLINLERSRDRREKMQARLAALGLDYTIFPAIDGRK
                     EWERLASQLDVDAFQRNVGRDVLPGEIGCYHSHLGVWKAFLETGDDVLLVLEDDVVFG
                     EDFISALHEAMRVCAHWDFLKLNKIRAEQPISQGRVGPYRLNAYLGAATGFGAYLIRR
                     DLVERALPAMLPITRPIDHEFDRIHVHRFRHFGLEPFPSDVRDESQSTITGTSFNSVR
                     KYPWYRRLPLYALRLRNRLGRLIYLATSGCLLPKTRRL"
     gene            1110..2219
                     /locus_tag="VIS73_07050"
     CDS             1110..2219
                     /locus_tag="VIS73_07050"
                     /inference="COORDINATES: protein motif:HMM:NF016903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HEY5762948.1"
                     /translation="MKLRRLMVPRWKMRRELRSLREQIAALPELIVGPLRKRRADRRF
                     PHDLRIADGAAPASARIAIYLTFQPNGLAESTLIACRHLAERGYAVLLVANAPIADAD
                     RERLAPHVWRFVQRVNFGYDFGGYRDGLQLLQHWQARPRYLIVLNDSIWFPLYPQEDL
                     IARMEGSEAAFVGAQRYVDHLDDLEERTGILMSFLFLFKAPLLESPEFRRFWRSYVPS
                     SSKVLTVRRGERRFSRQLIARGQTGDGIYSPVRFLQAMAQQSTEFLAKTLDYAAYTDA
                     DLERESGALLAEPVRDDSWRDRVLVHLRTTSGRRNFCSAFPYASIQALGVPFLKKNRA
                     ELQMRMRRQFLRAVAAGELPRPVEAIFREIERSVE"
BASE COUNT          401 a          772 c          757 g          443 t
ORIGIN      
        1 tgattgccgt gccgaagtcc tttgcggtca agtttccgac cgagttcatg ttcccgtggc
       61 ggccctggct ctacgtgcgt gcattgctgc cctgggttcg ccgtcgccgc gagcaactgg
      121 tcgcggtgac cctgaacggc atgtcgatca aacccgaaaa gctgatcgcg ctcgaagacg
      181 gcgcgatcgt cgtcgacgag aaggggcgga gcatggtctg gagcgagcgg acgatgggcg
      241 cgaacaaggc caagatcgcc gccttctacg ccggagttgt cggtcaatga agctgcctgg
      301 cctcgcggtt tacctgatca atctcgagcg ctcgcgcgat cggcgcgaga agatgcaggc
      361 acggctggcg gcgctgggtc tcgactacac gatctttccg gccatcgacg ggcgcaagga
      421 atgggaacgt ctggccagcc agctcgacgt cgacgccttc cagcgcaacg tcggacgcga
      481 cgtgctgccc ggcgaaatcg gctgctacca ctcgcatctg ggggtctgga aggccttcct
      541 ggagacgggc gacgacgttc tgctggtgct cgaggacgac gtcgtcttcg gcgaggattt
      601 catttcggcg ctgcacgagg ccatgcgcgt ctgcgcgcac tgggatttcc ttaagctcaa
      661 caagatccgc gccgaacagc cgatttcgca ggggcgggtc ggaccgtatc gcctgaacgc
      721 ctaccttggt gccgcgaccg gcttcggcgc ctacctgatc cggcgcgatc tggtcgagcg
      781 cgcactgccg gcaatgctgc cgatcacccg gccgatcgat cacgagttcg accggattca
      841 tgttcaccgc ttccgccatt tcggtctcga gccctttccc agcgacgtgc gcgacgagag
      901 ccagagcacg atcaccggta ccagtttcaa ttcggtacgc aagtaccctt ggtaccgtcg
      961 cctgccgctc tacgcgctgc gcctgcgaaa taggctcggt cgattgatct atctggcgac
     1021 gagcggatgc ctcctgccga agacgcgccg gctttgatcg gcggcacacc caatctgagc
     1081 agcgcggtct gcgatcggag ccgccggcga tgaagctgcg ccgcctgatg gtgccgcgct
     1141 ggaaaatgcg ccgcgagctt cgctcgctgc gcgaacagat cgccgcgttg cccgagctca
     1201 tcgtcggccc cttgcgcaag cggcgcgccg accgccgttt tccacacgat ctgcgcatcg
     1261 ccgacggcgc tgcgccggcg tcggccagga tcgcgatcta cctgaccttc cagccgaacg
     1321 gcctcgccga atcgaccttg atcgcctgcc ggcatctggc cgagcgcggc tacgcggtgc
     1381 tgctggtggc caatgcgccg atcgccgacg ccgaccgcga gcggttggcg ccgcatgtct
     1441 ggcgcttcgt ccagcgcgtg aacttcggct acgacttcgg cggctatcgc gacggactgc
     1501 agctcctgca gcactggcag gcgcggccaa ggtatctgat cgtacttaac gacagcatct
     1561 ggttcccgct ctatccgcag gaggacctga tcgcgcgcat ggagggctcg gaagcagcct
     1621 tcgtcggtgc gcagcgctac gtcgaccatc tcgacgacct cgaggaacga accggcatcc
     1681 tgatgtcctt tctcttcctg ttcaaggcgc cgctgctcga atcgcctgag ttccgccgct
     1741 tctggcgcag ctacgtgccc agcagcagca aggtgctgac cgtcagacgc ggcgagcggc
     1801 gcttctcgcg ccagctgatc gcccgcggcc agactggcga tggcatctat tcgccggttc
     1861 gcttcctgca ggcgatggcg cagcagtcca ccgaattcct ggcgaagacg ctggactacg
     1921 cggcctatac cgacgccgat ctcgagcgcg agtccggggc gctgctcgcc gaaccggtgc
     1981 gcgacgacag ctggcgcgac cgcgttctcg tgcacctgag aacgaccagc ggccggcgta
     2041 atttctgttc ggcctttccc tacgcgagta tccaggctct gggggtgcct tttctcaaga
     2101 agaaccgggc cgagctgcaa atgcgtatgc gccgccagtt cctgcgggcc gtcgcggcgg
     2161 gagagctgcc gcggcccgtg gaagcaattt ttcgcgagat cgagcgcagc gtcgaatgag
     2221 ctgggtaggc cggatcgagc ctgttcggcg accggcatgg gtttggcagg ccgggacgtc
     2281 aattcactgc aacgtataat cgcgccctgc tggcgacatg caaatcagaa tttgctaata
     2341 tttgacgagg ttactgcaaa tggaacaaac aat
//
LOCUS       DATDAA010000223        18817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_121722,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000223 DATDAA010000000
VERSION     DATDAA010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 18817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18817
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121722"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="VIS73_07055"
     CDS             complement(<1..1222)
                     /locus_tag="VIS73_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY5762949.1"
                     /translation="MQDPSSTRNGSAARGGPLAGVRVVEFGGIGPGPFCGMLLADMGA
                     EVTLIEKPQGTLGAQLFGGGRRTIANRGKRSIAVDLKADGARQMVQRLIRDADVLIEG
                     FRPGVMERLGFGPETCLADNPRLVYGRMTGWGQTGPLAPRAGHDANYVALSGVLDCGR
                     RHGEAPWAPPTVAGDMGGGLTLAWGIACALLEARRSGRGQVIDAAMSEASSQLAQGLF
                     NLEAYGQWPARQSPDWRHEAVLDSGAPFYDVYRCADGGWISVCPLEPQFYASFLQRLG
                     LAEDPDFQTAQYDNTHWRQQHARLTALFARMPRAHWEQVFADTDDCVWPVLSLAEAPR
                     HPHNRAREAFVERDGVTHPTPSPRLSVTPAAIGEPSPLVGEQTREILRDAGYADSEIE
                     HMVQGGTVVVGDGPV"
     gene            1417..2838
                     /locus_tag="VIS73_07060"
     CDS             1417..2838
                     /locus_tag="VIS73_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HEY5762950.1"
                     /translation="MIAPRHAYRHADGALFLTDGTTRRSSMAEAYKAYSLNNFRNIPQ
                     MAKVPEQIKFEIEVVGHVLPFKSNSHVIEHLIDWSRVPDDAMFRLTFPQRDMLKPAAF
                     DEMAALLKSGADAAAIKACAEKIRLTLNPHPAGQLEHNVPTIEGEPMYGMQHKYRETV
                     LFFPSQGQTCHAYCTFCFRWPQFVGMNELKFASKEVDKLVAYIRAHPHVTDVLFTGGD
                     PMVMAPKNLASYIDPLLEADLPNLRRIRIGTKALAFWPYKFTSDPHAGELLALFRRVT
                     DSGRQLAFMAHFNHPAEVAPAVVAEAAANIRDTGAVIRTQSPLLANINDDPQLWADMW
                     SRQVDLGMVPYYMFLARDTGAHDYFAVPLVRAWDIFRDAYQKVSGLCRTVRGPSMSAN
                     PGKIQVLGVSEAGGEKVITMRFLQGRNPDWVHRPFFAKYDEKATWIDDLRPAFGLEKF
                     FFENELEDFYREKLDAVTVGYFE"
     gene            complement(2869..3213)
                     /locus_tag="VIS73_07065"
     CDS             complement(2869..3213)
                     /locus_tag="VIS73_07065"
                     /inference="COORDINATES: protein motif:HMM:NF025062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HEY5762951.1"
                     /translation="MITETLTKAITAHMAWTQTFAEAIASGAHNDVILKQAGSDHRCA
                     FGQWLYGMDETAKASPAYRRVKDKHYSFHVEAGIVARLLLEADREAARKALEGAFAKA
                     SGELVQALVDWK"
     gene            complement(3327..4259)
                     /locus_tag="VIS73_07070"
     CDS             complement(3327..4259)
                     /locus_tag="VIS73_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762952.1"
                     /translation="MGTAADPASPGRACPLHYRYRPEDIAAAPLREAEILYVVGGLYG
                     NGPALAAVEAMAAAEAAYVTMVFNGDFNWFNVDDASFRAINERVLRHHATLGNVEAEL
                     FGDDGSAGCGCAYPEDVDDALVSRSNAIHARLRQTADRHPDLVARLAQLPMYARYRVG
                     GIHVAVVHGDCESLAGWRFAVDTSATSDEVAAAFRRSACELIASSHTCLPVMRRIDGA
                     SLLANNGAAGMPNFRDQHSGLLTRIAAIRSPHPPRYGASLAGIHVDALPIVYDHATWT
                     TDFLANWPPGSPAHQSYWPRIEQGPDFGIDQARP"
     gene            complement(4336..5730)
                     /locus_tag="VIS73_07075"
     CDS             complement(4336..5730)
                     /locus_tag="VIS73_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762953.1"
                     /translation="MSDLQGLFFWGFLVVFGVVMYLVSPRVKDDAGFFRGTDATGRAT
                     SGWALTMSIFISWIFAKSVTNAANLGAAYGIVGGLAYATYWLSIPVAGLVIYRLRTRE
                     GATGLVPFLIRKYGRLAALAFSAAILVRLFNEVWSNTAVVGGYYGASGSPGFIVAALG
                     FTAAVLFYSLKGGLRSSIFTDAIQAVVFVLFLGLVLAFVLPMHPTSALLSEGEFALNN
                     GGDLLLVALLQVLSYPFHDPVLTDRGFLTEEKTMLRSFIVAGLLGFVAILAFSLVGVH
                     ARLEGIAGGGNVPAAVARGLGFAGFFAMVAVMVSSAASTLDSTFSSLSKSVAQELPLL
                     AARQPSTNARRVGLAAMIVFAIVGNLPMIAGTDILKATTISGTMVIGLAPIFLCSRFV
                     SHSPLSFHLAFWSGMVLGLLLALGLIPQSWAIGTGKYALLLGTNAWGLLICTVGFFLP
                     LALRQATARAVRPA"
     gene            complement(5747..6760)
                     /gene="arsS"
                     /locus_tag="VIS73_07080"
     CDS             complement(5747..6760)
                     /gene="arsS"
                     /locus_tag="VIS73_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238282.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HEY5762954.1"
                     /translation="MHATLPLLAATDFPPITRRAVETLQVNLGYRCNQSCTHCHVNAG
                     PKRTEEMSAATIAELLACLDRNPGIRTLDLTGGAPELNANFRHLVREARARGLTVIDR
                     CNLTILSEPGQESLANFLAEQQVEIVASLPCYLEDNVDKQRGKGVFAASIAGLKALNA
                     LGYGHDSSRLVMNLVYNPQGAELPPPQASLEAAYKTYLNEHFGVVFNGLFTLANMPIQ
                     RFGSTLISKGQFNNYMLLLKAAHRDENLDGVMCRTLVSVDWQGYLYDCDFNQQLGLPL
                     EIRSPVRRHLRDLVATCLTEAPIRVAEHCWGCTAGQGSSCGGALEAETTAPHAAAPSC
                     GQQ"
     gene            7011..7808
                     /locus_tag="VIS73_07085"
     CDS             7011..7808
                     /locus_tag="VIS73_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY5762955.1"
                     /translation="MNSRAFDGAGMVAGNWQMIAVWDLPTRVFHWSLVLSFAGAYITG
                     EVERWGTLHALLGYVLLLLIGFRLVWGVIGSRYARFSAFVTAPVAVLRYLGSLLGGRP
                     EHHVGHNPAGAMAIVLLLALGLMVAVTGLATLKGIGGEWVEDIHEAAAQAMLAVVAIH
                     VAGVLVSSVLHRENLVRAMLTGRKHGHPAEGLASMHRLLGIALLLGLVALGAVWATGS
                     TARLPGLGDVEQLDREWQEEGRGEARERDGEDDGRRRGRGRGERDDD"
     gene            7904..10486
                     /gene="clpB"
                     /locus_tag="VIS73_07090"
     CDS             7904..10486
                     /gene="clpB"
                     /locus_tag="VIS73_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY5762956.1"
                     /translation="MRLDKLTTKFQQALADAQSLAVGHDNQMIEPAHLLLALLQQDDG
                     GSASLLAHAGVNVPALKADLDRAIDRLPQVEGHGGEVSIGRDLGNLLNLTDKAAQKRG
                     DQFIASEMFLLVLCDDKGEAGRLAKEHGLSRKALEAAIDAVRGGAGVESQDAESQREA
                     LKKYCIDLTERALAGKLDPVIGRDDEIRRVMQILQRRTKNNPVLIGEPGVGKTAIVEG
                     IAQRIINDEVPETLKGKKVLVLDMAGLIAGAKYRGEFEERLKAVLKDLAKDEGRIILF
                     IDEIHTMVGAGKAEGAIDAGNMLKPALARGELHCIGATTLDEYRKHIEKDAALERRFQ
                     KVLVDEPDVESTIAILRGLQEKYELHHGVDISDPAIVAAAELSHRYITDRFLPDKAID
                     LIDEAAARVKMEIDSKPESMDKLDRRLIQLKIEREAMKKEKDEASKKRLALIEDEITR
                     LQKEYSDLEEVWKAEKAQVQGSAHIKEEIDRLRAQMAELQRKGQYDKLAELQYGQLPA
                     LEAQLKAAEVSGDTAAKPNRLLRTHVGAEEIAEVVSRATGIPVSKMMQGEREKLLKME
                     EKLHERVVGQDEAVRLVADAIRRSRAGLSEESRPYGSFLFLGPTGVGKTELCKALAGF
                     LFDSEDHLIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILFDEV
                     EKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSQMIQSMSGDDYQVIK
                     LAVMAEVKTHFRPEFVNRIDEIVVFHALDEKHIAGIAKIQLAYLEKRLARLDMHLEVS
                     DAALAELATAGFDPVFGARPLKRAIQEKIENPLARKILEGEFAAKDSVRVDAGGGQLQ
                     FTKD"
     gene            10595..10804
                     /locus_tag="VIS73_07095"
     CDS             10595..10804
                     /locus_tag="VIS73_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEY5762957.1"
                     /translation="MGIMGPVDTSYVSEHTKFINEQLKKNPEWEADQRRGRAIWWDKP
                     QTEEAKKVAEAADVKAKAYPYDVHF"
     gene            10916..11428
                     /locus_tag="VIS73_07100"
     CDS             10916..11428
                     /locus_tag="VIS73_07100"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEY5762958.1"
                     /translation="MNSVLRLAGLALIVLASGALRAAEIDPGKRALIDRLMEQTGQSA
                     AVVGKQYADLFVREIDELLKQANPGVNPRVFDIMREEVGLTIEDELGGHGTFMDTMYP
                     VYDLHFTADELRQMIAFNSTPLGQKMLRVLPQVTQEGMAAGQTWGRTLGIKVYGRVVI
                     RLLEEGVRLP"
     gene            complement(11510..14194)
                     /locus_tag="VIS73_07105"
     CDS             complement(11510..14194)
                     /locus_tag="VIS73_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEY5762959.1"
                     /translation="MNDQHYLTTLFEPKTVAVVGASERDNAVGNVVFRNLLDSGFKGR
                     LFAVNPKHPTIFGQQSFKSVEDVGVRLDLAVIATRPQTVPSIIEQCGRAGVRNAIVLA
                     SGFAESGHIGANIERKMLEVARSYGVRILGPNCLGIMRPNLGLNVTFTRASCETSNLA
                     LVSQSGAMCAAMLDWAMANNIGFSSVISLGTQADVEFGEILDYLIYDARTHYILLYVE
                     SIRNARRFMSALRGAARIKPIILLKAGRHAGGERAVRTHSGMAAVSDTVFDAAIRRAG
                     VVRVSNVGQLFFAAKAISSRYRPVGTRLAIITNGGGPGALAADRAGDLGVPLAELSEA
                     TVKELNQCLPTNWSHDNPVDLLGDATPERYRNAIVAVAKDPQVDAILVILVPAAMTEP
                     EKVARAIIDIHKELGKPISCCWMGEAHVAESRRMLMAAQVPTYRTPDTAIELHASIAT
                     YYHNQKLLLQTPTPAVDAPPPTHEGARMLIEAVLAEKRKVLSEMESKAVLRAFKIPVA
                     QTMVARTPTEALLLAEQIGFPIAMKVDSPDLAQKSDAGGVRLNINSAAAVRNAYLDIL
                     ETVKTRNPDIRINGVSIEPFLARPHGRELLVGVVRDRIFGPVIVFGAGGNDVEVFSDR
                     AIALPPLNRFLARDLIHSTRVSGILGEFRNMPAIDMDALKSVLLAISDIVCELPHIQS
                     IDLNPLIVDEQGAIAADARIVIDHAPTGDRYAHMAIHPYPAHLIQHWPQNDGSTVLIR
                     PIRPEDADMLQAFVRSMSDESRYMRFMDTIRELSMPMLVRFTQIDYDREMALTALAQD
                     DDHEKIIGVARYATNPDGNSVEFALAVADEWQKHGIGPRLMTELIDCAKSKGFDTIVG
                     DVLAANEKMLRLMTNLGFTIHPHPEDNAVKRVIKTLQN"
     gene            complement(14280..14912)
                     /locus_tag="VIS73_07110"
     CDS             complement(14280..14912)
                     /locus_tag="VIS73_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY5762960.1"
                     /translation="MRPRAVLFDLGGVLLDVHLDRTLAHWAAAADPSGRLPADELRGR
                     LREDETYRAFECGHCDAPDFFAALTKQLSITLDADSWRAGWLAMLGQVRPEMARLVEE
                     LAGEMPLFLFSNTNATHHAVWQQSCAPLLGHFRECFVSHQIGARKPDGAAFAAVVQRI
                     GLPAADILFLDDLPANVAGARATGLRAERVASHSEAIAALACHGIPADRA"
     gene            complement(14926..15798)
                     /locus_tag="VIS73_07115"
     CDS             complement(14926..15798)
                     /locus_tag="VIS73_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEY5762961.1"
                     /translation="MSDSRYQRFLFEQLDIRGAIVHLGEAWRTMIAERGYPPAISQML
                     GEMTAITVLLGGQLKHEGRLTFQARGNGPVGLLVIDCESTDEALRLRGMARADWPADM
                     PAAKVPEMFGDGRLLMSIDLPDSRVPFQSIVPLDGATIGEVFESYLRQSEQQASRLVI
                     AAGPTAACGVFVQKMPDADRRDPDGWTRIGHLLDTLKPEELLELAPEALLQRLFYEET
                     VRVFTPREVVHHCPEDWEKVRATLTALGRSEVEALLDEQGEIVIRDDICNREYRLDRA
                     MALEFLEAAEHRLH"
     gene            complement(15795..16160)
                     /locus_tag="VIS73_07120"
     CDS             complement(15795..16160)
                     /locus_tag="VIS73_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEY5762962.1"
                     /translation="MITVYGIRNCDTMKKTFAWFEAQGIEYRFVDYKGTAGAERLPDW
                     IAALGWEALVNRRGLTWRRLAESERLDLDEDRALALMRAHPTLVRRPVIEHDGGVLLG
                     FDPEQFAATLTAVKGRARR"
     gene            complement(16157..18469)
                     /locus_tag="VIS73_07125"
     CDS             complement(16157..18469)
                     /locus_tag="VIS73_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HEY5762963.1"
                     /translation="MLPSIELRIAQQLDVNVAQVTAAARLLDEGATVPFIARYRKEAT
                     GGLDDTQLRTLAERLEYLRGLEERRAVILDSVAEQGKLSDALRAALLGAETKQALEDL
                     YLPYKQKRRTKAQIAREAGIEPLADLLIDDPMTVPEAAAAGFINAEAGFADTKAVLDG
                     ARQILMERFAEDATLVAGIRAYFSEHAILCGRLVAGKEQEAAKYRDYHDFREHWTTVP
                     SHRALALLRARNEGLLQVSLALDSDLADDIDPRELNPCEQRVAAHTGIRNEGRPADVW
                     LRDTARWTWKVKLALHLEMELMGALRERAEREAIRVFGKNLQDLLLAAPAGRRPTLGL
                     DPGIRTGVKVAAVDGTGKLVATDTVYPHEPRRDWDGSIARLAAIAAAQHIELVAIGNG
                     TASRETEKLVADLIARHPELKLTPVVVSEAGASVYSASELAAREFPQLDVSLRGAVSI
                     ARRLQDPLAELVKIDPKSIGVGQYQHDVSQTALARSLGDVVEDCVNAVGVDVNTASAP
                     LLARVSGLSPALAEGIVGWRDANGPFPSRTALLAVPRLGDKTFEQAAGFLRITDGDNP
                     LDGSGVHPESYPLVEKILVDLGRPLRALLGDSRTLKGIEAERYADERFGVPTVRDILR
                     ELEKPGRDPRPQFRTAKFSENVRELSDLEPGMQLEGTVTNVAAFGAFVDIGVHQDGLV
                     HVSALADRFVRDPREVVKVGDIVRVKVVSVDLPRQRIALTMRLKAPVDPTTTPAPAPR
                     GSTRSPQRRAAQSPPLAAGAMAAAFARLKT"
     gene            complement(18548..>18817)
                     /gene="fliW"
                     /locus_tag="VIS73_07130"
     CDS             complement(18548..>18817)
                     /gene="fliW"
                     /locus_tag="VIS73_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HEY5762964.1"
                     /translation="IALQIADPGAYGLHYELELNDDEAALLQAADPTDIALMLVLYKQ
                     EDAGAPIRANLQAPLLINTRSRRGLQKVMPRIEPRVTLKDLSVPA"
BASE COUNT         3182 a         5832 c         6631 g         3172 t
ORIGIN      
        1 ggaccggacc gtcgccgacg acgacggtgc cgccttggac catatgctcg atctcgctgt
       61 cggcgtatcc ggcatcacgg aggatttcgc gcgtctgctc gccgaccagc ggcgagggtt
      121 cgccgattgc ggcgggggtc accgacaggc gcggcgaggg cgtcggatgg gtcacgccgt
      181 cgcgttcgac gaaggcctcg cgggcacggt tatgcgggtg ccgcggtgcc tcggcaagcg
      241 agagcaccgg ccagacgcaa tcgtcggtgt cggcgaaaac ctgctcccag tgggcgcgtg
      301 gcatgcgcgc gaagagcgcg gtgagtcgcg cgtgctgctg gcgccagtgg gtgttgtcgt
      361 attgcgcagt ctggaagtcg gggtcttcgg cgagccctag acgctgcagg aagctcgcgt
      421 agaactgcgg ttcgagcggg cacaccgaga tccagccgcc gtcggcgcag cgatagacat
      481 cgtagaaggg cgcgcccgaa tcgagcaccg cctcgtgccg ccagtccggc gactgccgag
      541 ccggccactg gccgtaggct tcgaggttga acaggccctg cgcgagttgc gacgaggctt
      601 cgctcatcgc ggcgtcgatg acttggccac gaccgctgcg gcgagcctcg agcaaggcgc
      661 aggcgattcc ccaggccagc gtcaggccac cgcccatgtc gccggcgacc gtcggcggtg
      721 cccagggcgc ctcgccgtgc cggcggccgc agtcgagcac gccggagagc gcgacgtagt
      781 tggcgtcgtg gccggcacgc ggcgccagcg gcccagtctg gccccagccg gtcatgcggc
      841 catagaccag tcgtgggttg tcggcgaggc aggtttcggg gccaaaaccg aggcgttcca
      901 tgacgcccgg gcgaaatccc tcgatcagca cgtcggcgtc gcgaatcagt cgctggacca
      961 tttgtcgcgc gccgtcggcc ttgaggtcga cggcgatcga gcgcttgccg cggttggcga
     1021 tcgtccgccg gccgccgccg aagagctggg cgccaagtgt gccctggggt ttttcgatca
     1081 gggtcacctc ggcgcccatg tcggcgagca gcatgccgca gaaggggccg gggcctatgc
     1141 cgccgaactc gacgacacga acgccggcaa gcggcccccc gcgggcggcg cttccgttgc
     1201 gggtggatga tgggtcctgc atcgttgtta cctctccctg atttgctgct gcgagtcatt
     1261 tcgcggcgag gcggtatact tgcatgagga attatccatt gcgtcccgcc gaattcgccc
     1321 cggcgggggc tccggcgacg cgaatcaagg acggcaggtc cagcgcgccg gcccggtcga
     1381 agccggacac aagaagacac aagaccttcg gaaacgatga tcgccccgcg acatgcctac
     1441 cggcacgccg acggcgcgct cttcctcacc gacggcacca cgaggagatc aagcatggca
     1501 gaagcctaca aggcgtattc gctgaacaat tttcgcaaca tcccgcaaat ggcgaaggtg
     1561 ccggagcaga tcaagtttga gatcgaggtg gtcgggcacg tgctgccctt caagtcgaac
     1621 agccatgtca tcgaacatct gatcgactgg tcgcgggtac ccgacgatgc gatgttccgc
     1681 ctgaccttcc cgcaacgcga catgctgaag ccagccgcct tcgacgagat ggccgcgctg
     1741 ctcaagtcgg gtgccgacgc ggcggcgatc aaagcctgcg ccgagaagat tcgcctgacg
     1801 ctcaacccgc acccggcagg ccagctcgag cacaacgtgc cgaccatcga aggcgagccg
     1861 atgtatggca tgcagcacaa gtatcgcgag acggtgctgt tcttccccag ccaagggcag
     1921 acctgtcacg cctactgcac cttctgtttc cgctggccgc agttcgtcgg catgaatgag
     1981 ctgaagtttg cctccaagga ggtggacaag ctggttgcct acatcagggc gcatccgcat
     2041 gtcaccgacg tgctgttcac cggcggcgac ccgatggtga tggctccgaa gaacctggcg
     2101 agctacatcg acccgctgct cgaagccgat ctgcccaatc tgcgccgcat ccgaataggc
     2161 accaaggcgc tcgccttctg gccctataag ttcaccagcg acccgcacgc tggcgagttg
     2221 ctggcgctgt tccgccgcgt caccgactcg ggtcggcaac tcgccttcat ggctcacttt
     2281 aaccatccgg ccgaggtggc gccggccgtc gtcgccgaag ccgccgccaa catccgcgac
     2341 accggtgcgg tgatccgcac acagtctccg ctcctggcga acatcaacga cgatccgcag
     2401 ctgtgggccg acatgtggag ccgtcaggtc gacctcggta tggtgccgta ttacatgttc
     2461 ctcgcgcgcg acacgggcgc ccacgactac ttcgcggtgc cgctggtgcg cgcctgggat
     2521 attttccgcg acgcctacca gaaggtcagc ggcctctgcc gcacggtacg cggaccgtcg
     2581 atgtcggcga acccgggcaa gattcaggtg cttggtgtgt ccgaggcggg gggcgaaaag
     2641 gtgatcacga tgcgcttcct gcaggggcgc aacccggact gggtgcatcg ccccttcttt
     2701 gccaagtacg acgagaaggc gacctggatc gacgacctca ggcctgcctt cggcctggag
     2761 aagttcttct tcgagaatga actcgaggac ttttaccgcg agaagctcga cgcggttacc
     2821 gtcggatact tcgagtaacg cactgccgat ccgccctcag gcgccgctct acttccagtc
     2881 gaccagcgcc tgaaccagct cgccggaagc cttggcgaag gctccttcaa gggcctttcg
     2941 ggctgcctct cggtcggctt caaggagcag gcgggcgacg atgccggcct cgacatgaaa
     3001 gctgtagtgc ttgtccttga cgcggcggta agccgggctg gccttcgccg tttcgtccat
     3061 gccgtagagc cactgcccga atgcacagcg atggtcgctg ccggcctgct tgagaatcac
     3121 gtcgttgtgg gcgccgctgg cgatcgcttc ggcgaaggtt tgcgtccagg ccatgtgcgc
     3181 ggtgattgcc tttgtcagcg tttcggtaat catcgatccg agtcccgatg ccaattggca
     3241 cctgttcgtc attgacgtga ggttagcaga ccttggacgg ctgcgaatgc cacggccact
     3301 ggcagtcggc aggatgcgcc ggtcatctac gggcgggcct gatcgattcc gaagtcggga
     3361 ccttgttcga ttcgcggcca gtaggactgg tgtgccggcg accccggcgg ccagttggcg
     3421 aggaagtcgg tagtccaggt ggcgtggtcg tagacgatcg gtagcgcatc gacgtgaatg
     3481 ccggcgaggc tcgccccgta gcgcggtggg tgcggcgacc ggattgcggc aatgcgcgtc
     3541 agcaggccgc tgtgttggtc gcggaaattc ggcatgccgg ccgcgccgtt gttggcgagc
     3601 aggctggcac cgtcgatccg ccgcattacc ggaagacagg tgtgtgagct ggcgatgagc
     3661 tcgcaggcgc tgcggcggaa agccgcggcg acttcatcgc tcgtcgccga ggtgtcgacg
     3721 gcaaagcgcc acccggcgag cgactcgcag tcgccgtgaa caactgcgac gtggataccg
     3781 ccgacgcggt agcgcgcgta catcggcagt tgcgccagcc gtgcgacgag gtcggggtga
     3841 cggtcagccg tctgccgcag ccgggcatgg atcgcgttcg agcgcgacac cagcgcgtca
     3901 tcgacgtcct cgggataggc gcagccacag ccggcgctgc cgtcgtcgcc gaacagttca
     3961 gcctcgacgt tgccgagcgt cgcgtggtga cggaggacgc gctcgttgat cgcgcgaaag
     4021 cttgcatcgt cgacgttgaa ccagttgaaa tcaccgttga ataccattgt cacgtatgcc
     4081 gcttcggctg ccgccatcgc ctcgacggct gccagcgccg gcccgttgcc gtataggccg
     4141 ccaacaacgt agagaatctc cgcctcgcgc agcggcgcag ccgcaatgtc ttccgggcgg
     4201 tagcggtagt gcagcgggca cgcgcggccc ggcgaagccg ggtccgcggc ggtacccaaa
     4261 cggcccggcg aagatgagtc cccggccgtc gctaatcggg tcggcgtgtt gtcagcttgc
     4321 acgcgtcttg ccacctcagg ccgggcggac cgccctggcc gtcgcctgcc gcaacgcgag
     4381 cggcaggaag aagccgacgg tacagatgag cagaccccag gcgttggtgc cgagcagcag
     4441 cgcgtacttg ccggtgccga tcgcccagct ctgcggaatc aggccgagcg cgagcaggag
     4501 gccgaggacc atgccgctcc agaaggcgag gtggaaggac agcggcgagt gcgagacgaa
     4561 gcgcgagcag agaaagatcg gcgccagccc gatcaccatc gtgccgctga tcgtcgttgc
     4621 cttgaggatg tcggtaccgg cgatcatcgg caggttgccg acgatggcaa atacgatcat
     4681 cgccgccagg ccgacgcgac gtgcgttcgt tgatggctgt cgcgccgcga gcagcggtag
     4741 ctcctgcgcc accgatttcg acagcgagga gaaggtcgag tcgagcgtcg acgcggccga
     4801 cgagaccatc accgcgacca ttgcgaagaa gccggcgaag cccagcccac gcgccacggc
     4861 cgcgggcacg ttgccgccgc cggcgatccc ttcgagccgc gcgtgcacgc cgaccaggct
     4921 gaaggcgagg atggcgacga agccgagcag gccggcgacg ataaaggagc gcagcatggt
     4981 cttttcttcg gtgaggaagc cgcggtcggt gagcacggga tcatggaacg ggtaggacag
     5041 cacctgcaga agcgccacca gcagcaggtc gccgccgtta ttcagtgcga actccccttc
     5101 gctcagcagt gcgctggtcg ggtgcatcgg cagcacgaag gcgagtacga ggcctaggaa
     5161 gagcacgaag acgaccgcct ggatcgcatc ggtgaagatc gagctgcgca ggccgccctt
     5221 gaggctgtag aagagcacgg cggcagtgaa gccgagcgca gcgacgatga atcccggcga
     5281 gccgctcgcg ccgtagtagc cgccgaccac agccgtgttg ctccacacct cgttgaacag
     5341 gcggacgagg atcgccgccg aaaacgccag cgccgccagt cgcccgtact tgcggatgag
     5401 gaagggcacc agcccggtcg cgccctcgcg cgtgcgcaac cggtagatga caagacccgc
     5461 caccggaatc gacagccagt aggtggcgta ggcgagtccg ccgacgatgc cgtaggcggc
     5521 gccgaggttg gcggcgttgg ttactgactt ggcgaagatc caactgatga agatgctcat
     5581 cgtcagtgcc cagcccgacg tcgcgcggcc ggtggcatcg gtgccgcgga agaagccggc
     5641 atcatccttc acgcgcggcg agaccagata catcacgacg ccgaagacga cgaggaaacc
     5701 ccagaagaac agcccctgca gatcgctcat ggcgactggc cccggcctat tgctggccgc
     5761 agctcggtgc cgcggcgtgc ggtgctgtcg tttcggcttc cagcgcgccg ccacagctcg
     5821 atccttggcc cgccgtgcag ccccagcagt gctcggcaac gcggatcggc gcctcggtca
     5881 ggcaggtggc gacgaggtca cgcaggtggc ggcggaccgg cgagcggatt tccaatggca
     5941 gaccgagctg ctggttgaaa tcgcagtcgt agagatagcc ctgccagtcg acgctgacca
     6001 gcgtgcggca catcacgcca tcgaggttct cgtcgcggtg cgcggccttt agcagaagca
     6061 tgtagttgtt gaactggccc ttggagatca gcgtcgagcc gaatcgctgg atcggcatgt
     6121 tggcgagcgt gaacaggcca ttaaagacga cgccgaagtg ctcgttcagg taggtcttgt
     6181 aggcggcctc gaggctggcc tgtggcgggg gcagctccgc gccctgcggg ttgtagacaa
     6241 ggttcatgac cagtctgctg ctgtcgtggc cgtagcccag cgcattcagg gccttaaggc
     6301 cggcgatgct ggcagcgaag acgcccttgc cgcgctgctt gtcgacgttg tcctcgaggt
     6361 agcagggcag cgaggcgaca atctcgacct gctgttcggc gaggaagttg gcgaggcttt
     6421 cctgccccgg ttccgagagg atcgtcaggt tgcagcgatc gatgaccgtg agtccgcgcg
     6481 cgcgcgcctc gcgcaccaga tggcggaagt tggcgttgag ttcgggtgct ccgccggtca
     6541 ggtcgagcgt gcgaatgccg ggattgcgat caaggcaggc gagcagctcg gcgatcgttg
     6601 cagccgacat ctcttcggta cgtttggggc cggcgttgac gtgacagtgc gtgcagctct
     6661 ggttgcagcg gtaaccgaga ttgacctgga gcgtttcgac ggcacgccgg gtgattggcg
     6721 ggaagtcggt ggcggcgaga aggggcagcg ttgcgtgcat ggttatcctt gtcgtgacgg
     6781 gtcgttgccg tcgtggtgac cttgggtcgg tcggcagcgg ggatttctta caggccggta
     6841 gcgcggcagc cgcggcggtc gttcggcgaa cggcgccgaa aggatcgata cggagcctaa
     6901 accggcgccg gcggaaagac aattcgctcg ccaaaggggg tagcgacggc ggtgcggccg
     6961 agggctacag tgaaaacgtg ggcccgttca gggtcgatgg gagggcaaca atgaacagca
     7021 gggccttcga cggtgccggc atggtcgccg gcaactggca gatgatcgcg gtctgggatc
     7081 tgccgactcg ggtgttccat tggagtctgg tgctgtcctt tgccggtgcc tacatcacgg
     7141 gcgaagttga gcgctggggg acgctgcacg cgctgctggg ctacgtactg ctgctgttga
     7201 tcggcttccg tctggtctgg ggcgtgatcg gcagtcgcta cgcgcgcttc tcggccttcg
     7261 tcaccgctcc ggtggcggtg ttgcgctacc tcggctcgct cctcggcggc cggccagagc
     7321 accacgtcgg ccacaacccg gcgggcgcga tggcgatcgt cctgctgctc gcgctgggcc
     7381 tgatggtcgc ggtgactggg ctggcgacgc tcaaaggcat aggcggcgaa tgggtcgagg
     7441 atattcacga agcggctgca caggcgatgc tcgccgtcgt cgcgatccac gttgctgggg
     7501 tcctcgtcag cagcgtcctg caccgcgaga acctggtccg ggcgatgctc acgggccgca
     7561 agcacggcca cccggccgaa gggctcgctt cgatgcatcg cttgctcggc atcgcgctgt
     7621 tgctcggtct ggtcgcgctc ggtgctgtct gggcgactgg ttcgacggcc cgcctgcccg
     7681 gactcggcga cgtcgaacag ctcgatcgcg aatggcagga ggagggccgc ggcgaagcgc
     7741 gggagcggga cggggaagac gatgggcgcc gccgggggcg cggccgtggc gagcgcgacg
     7801 acgactaggc cctggcgcgg ctgttggccc cgatgcagtt gaaataccgg ggccgagccc
     7861 cacttgtcgg gcataggcat ttcattctag ggagaccgcc aagatgcgac tcgacaaact
     7921 gacgaccaag ttccagcagg cgctggccga tgcccagagt ctggcggttg gccacgacaa
     7981 ccagatgatc gagccggcgc acctgctgct ggccctgctg cagcaggacg acggcggttc
     8041 tgcttcgctg ctcgcgcacg ccggggtcaa cgtgccggcg ctgaaagccg acctggaccg
     8101 cgcgatcgac cggctgccgc aggtcgaggg tcacggcggc gaggtctcga tcggccgcga
     8161 cctcggcaat ctgctcaacc tcaccgacaa ggccgcgcag aagcgcggtg accagttcat
     8221 cgccagcgag atgttcttgc tcgtcctctg cgacgacaag ggtgaggcgg ggcggctggc
     8281 gaaggagcac ggcctgtcgc gcaaggcgct cgaggcggcg atcgatgccg ttcgcggtgg
     8341 cgccggcgtc gagagccagg acgccgaaag ccagcgcgag gcgctgaaga aatactgcat
     8401 cgacctcacc gagcgcgcgc tcgccggcaa gctcgacccg gtgatcggtc gcgatgacga
     8461 aatccgccgt gtcatgcaga tcctccagcg gcggaccaag aacaacccgg tgctgatcgg
     8521 cgagcctggc gtcggcaaga cggcgatcgt cgagggcatc gcccagcgca tcatcaacga
     8581 cgaggtcccc gagaccctga agggcaagaa ggtgctggtg ctcgacatgg ccgggctgat
     8641 tgccggcgcc aagtaccgcg gcgagttcga ggagcggctg aaggccgtgc tcaaggacct
     8701 cgccaaggac gagggccgga tcatcctgtt catcgacgag attcacacga tggttggcgc
     8761 cggcaaggcc gagggtgcca tcgacgccgg caacatgcta aagccggcgc tggcgcgcgg
     8821 cgagctgcac tgcatcggcg cgacgacgct cgacgagtac cgcaagcaca tcgagaagga
     8881 cgccgcgctc gagcgccgct tccagaaagt gctggtcgac gagcccgacg tggaatcgac
     8941 catcgccatc ctgcgcggcc tgcaggaaaa atacgagctg caccacggcg tcgacatcag
     9001 cgacccggcg atcgtcgccg cggccgagct ttcgcatcgt tacatcaccg accgttttct
     9061 gcccgacaag gcgatcgacc tgatcgacga agcggcggcg cgggtcaaga tggaaatcga
     9121 ctccaagccg gaatcgatgg acaagctcga ccggcgattg atccagttga agatcgaacg
     9181 cgaggcgatg aagaaggaga aggacgaggc ctcgaagaag cgccttgccc tgatcgagga
     9241 cgaaatcaca cggctgcaga aagagtattc ggaccttgaa gaagtctgga aggccgagaa
     9301 ggcgcaggtc cagggttcgg cgcacatcaa ggaagagatc gaccgcctgc gtgcacagat
     9361 ggctgagctg cagcgcaagg gccagtacga caagcttgcc gagttgcagt acgggcagct
     9421 gccggcgttg gaggcgcagc tcaaggccgc cgaagtctcg ggcgacacgg ccgcgaagcc
     9481 gaatcgcctg ctgcgcacgc atgtgggcgc cgaggaaatc gccgaagtcg tgtcgcgcgc
     9541 cactggcatc ccggtgtcga aaatgatgca gggcgagcgc gagaaactgc tgaagatgga
     9601 agaaaagctg cacgagcgcg tcgtcggcca ggacgaggcc gtgcgcctcg ttgccgacgc
     9661 gatccgccgc tcgcgcgctg ggctgtcgga agagtcacgc ccctatggca gcttcctctt
     9721 cctcggtccc accggcgtcg gcaagaccga gctctgcaag gcgttggctg gcttcctctt
     9781 tgattccgaa gaccacctga tccgcatcga catgagcgag ttcatggaga agcactcggt
     9841 cgcccggctg atcggcgcgc cgccggggta tgtgggctat gaggagggcg gctatctcac
     9901 cgagcaggtc cgccgcaagc cctacagcgt gattctcttc gacgaggtcg agaaggcgca
     9961 ccccgatgtc ttcaacgtcc tgctgcaggt acttgatgat ggccgcatga ctgacggtca
    10021 gggtcgtacc gtcgatttca agaacaccgt aatcgtcatg accagcaacc tcggcagtca
    10081 gatgatccag tcgatgtcgg gcgacgacta ccaggtcatc aagctcgccg tgatggccga
    10141 agtgaagacg catttccggc ccgagttcgt caatcgcatc gacgagatcg tcgttttcca
    10201 tgcgcttgac gagaagcaca tcgccggcat cgcgaagatc cagctcgcct atctggagaa
    10261 gcggctggcg cgcctggata tgcatctcga ggtctccgat gccgcactgg ccgaactggc
    10321 gaccgccggc ttcgacccgg tattcggcgc ccggccgctg aagcgggcga tccaggagaa
    10381 gatcgaaaat ccgctggcgc ggaagatcct cgaaggcgag tttgccgcca aggatagcgt
    10441 ccgcgtcgat gcgggcggtg gtcagctgca attcacgaag gactgaggtc gacatccagt
    10501 cacccggcgg gcgcgctata atccgcgccc gccggttggt gaagcgtcac gccgcgcacc
    10561 acgcagtcag tacagacacg aacaggagca agtcatggga atcatgggtc cggtcgacac
    10621 cagctacgtg tccgaacaca ccaaattcat caacgagcag ctgaagaaga accccgagtg
    10681 ggaagccgat cagcggcgcg gccgggcgat ctggtgggac aagccgcaaa ccgaagaagc
    10741 taagaaggtg gctgaggccg ccgacgtcaa ggccaaggcc tatccctacg acgtgcactt
    10801 ctgagcaggg gtgtagcgaa aaaagcccgg caagccgggc ttttttcatt ggtgcgcctg
    10861 ggtggatgaa acaatcgtct cccccggtta atcggctggt cgcaaggaga agcgattgaa
    10921 ttcagtactg aggttggccg ggctcgccct gatcgtgttg gccagcgggg cgttacgcgc
    10981 tgccgaaatc gacccgggca agcgggcctt gatcgaccgc ctgatggagc agaccgggca
    11041 gtcggccgct gtggtcggca agcagtacgc cgaccttttc gtgcgcgaga tcgacgagct
    11101 gctcaagcaa gcgaatcccg gcgtcaaccc tcgggtcttc gacatcatgc gcgaggaggt
    11161 cggcctaaca atcgaagacg aactgggcgg tcacggaacg ttcatggaca cgatgtatcc
    11221 ggtctacgat ctccatttca cggccgatga gctgcgccag atgatcgcct tcaacagcac
    11281 accgctcggg cagaagatgc ttcgcgtcct gccgcaggtg acgcaggagg gcatggccgc
    11341 cggtcagaca tgggggcgaa cgctgggcat caaggtgtac ggccgcgtcg ttatccgtct
    11401 gctcgaggaa ggtgtgcgcc tgccgtgatc aagcccgccg ccccttgcgg cgggcaccct
    11461 tcgccggccg ccggttctgg ctggcggccg atgacggaaa tcggctcgct tagttctgca
    11521 gcgtcttgat gacgcgtttg acggcgttgt cttcggggtg ggggtggatg gtgaagccga
    11581 ggttggtcat cagccgcagc atcttctcgt tggcggcgag gacgtctcca acgatggtgt
    11641 cgaaaccctt gctctttgcg cagtcgatca gctcggtcat cagccgcggg ccgatgccgt
    11701 gcttttgcca ttcgtcggca acggcgagcg caaattcgac tgaattgccg tcggggttgg
    11761 tcgcgtagcg ggcgacaccg atgatctttt cgtggtcgtc gtcctgggcc agcgcggtga
    11821 gtgccatctc gcggtcgtag tcgatctgcg tgaagcggac cagcatgggc atcgacaact
    11881 cgcggatcgt gtccatgaac cgcatgtagc ggctctcgtc tgacatgctg cggacgaagg
    11941 cctgcagcat gtccgcgtcc tccggacgaa tcgggcgaat cagaacggtt gacccgtcgt
    12001 tctgcggcca gtgctggatc aggtgcgccg ggtaggggtg gatcgccatg tgcgcatagc
    12061 ggtcgccggt gggcgcgtgg tcgatgacga tccgcgcatc ggcggcgatt gcgccctgtt
    12121 cgtcgacgat cagcgggttg aggtcgatcg actggatgtg cggcaactcg cagacaatat
    12181 cggagatcgc cagcagcacg ctcttcagcg cgtccatgtc gatcgccggc atgttgcgga
    12241 attcgccgag gatgccggag acgcgtgtcg aatggatcag gtcgcgggcg aggaagcggt
    12301 tgagcggcgg cagcgcgatg gcgcggtcgc tgaagacttc gacatcgttg ccgccggcgc
    12361 cgaagacgat gaccgggccg aagatgcggt cgcggacgac gccgacgagc agctcgcggc
    12421 cgtgcgggcg ggcgaggaag ggctcgatcg agacgccgtt gatgcggatg tcggggttgc
    12481 gcgtcttgac cgtttcgaga atgtcgaggt acgcgttgcg gaccgcggcg gcgctgttga
    12541 tgttgaggcg gacgccgccg gcatcggact tctgcgcgag atcgggcgag tcgaccttca
    12601 tcgcgatggg aaagccgatt tgctcggcga gcagcagcgc ttcggtcggg gtgcgcgcga
    12661 ccatggtctg cgcgaccgga atcttgaagg cgcgcagcac ggccttcgat tccatctcgg
    12721 agagtacctt gcgcttctcg gcgagcaccg cttcgatcag catgcgcgcg ccctcgtggg
    12781 tcggcggcgg cgcgtcgacg gccggagtcg gcgtctgcag cagcagtttc tggttgtgat
    12841 agtaggtggc gatcgaggcg tgcagttcga tcgcggtgtc gggggtgcgg taggtcggca
    12901 cctgcgcggc catcagcatg cgccgcgatt ctgcgacgtg cgcctcgccc atccagcagc
    12961 aggagatcgg cttgccgagt tccttgtgga tgtcgatgat cgcccgcgcg accttctcgg
    13021 gctctgtcat cgccgccggt acgaggatga ccaggatcgc atcgacctgc gggtccttgg
    13081 caacggcgac gatggcgttg cggtagcgct cgggcgtcgc gtcgccgagc aggtcgaccg
    13141 ggttgtcgtg cgaccagttg gtcggcaggc actggttaag ttccttgacc gtcgcttccg
    13201 agagctcggc gagcggcacg ccgaggtcgc cggcgcggtc ggcggcgagg gcgccggggc
    13261 cgccgccgtt ggtgatgatc gccagccggg tgccgaccgg acgatagcgg ctcgagatcg
    13321 ccttggcggc gaagaacagc tggccgacgt tggagacgcg gacgacgccg gcgcggcgga
    13381 tcgcggcatc gaacaccgtg tcggagactg ccgccatgcc cgagtgggtg cgcacggcac
    13441 gctcgccgcc ggcgtggcgg ccggccttga gcaggatgat cggcttgatc cgggcggcgc
    13501 cgcgcagcgc gctcatgaag cgtcgggcgt tccggatgct ctcgacgtag agcaggatgt
    13561 aatgggtgcg ggcgtcgtag atcaggtagt cgaggatttc gccgaactcg acgtcggcct
    13621 gcgtgccgag cgagatgacc gaggaaaagc cgatgttgtt ggccatcgcc cagtcgagca
    13681 tcgccgcgca catcgcgccc gattgcgaga cgagcgcgag gttgctggtt tcgcacgagg
    13741 cgcgcgtgaa ggtgacgttg aggccgaggt tcggccgcat gatgccgagg cagttggggc
    13801 cgaggatgcg cacgccgtag ctgcgggcga cttcgagcat cttgcgttcg atgttggcgc
    13861 cgatgtggcc gctttcggca aagcccgagg cgagcacgat ggcgttgcgc acgccggccc
    13921 ggccgcactg ctcgatgatc gatggcactg tctgtggacg cgtggcgata acggcaagat
    13981 cgagacgaac gccgacgtct tcgacgcttt taaacgattg ttgaccgaag atcgtcgggt
    14041 gtttcgggtt gacggcgaac agccggccct tgaagccgga atcgagcagg ttgcggaaca
    14101 ccacgttgcc gaccgcgttg tcgcgctcgg aggcgccgac aacggcaacc gtcttcggct
    14161 cgaatagcgt ggtcaggtag tgctggtcgt tcattacgtt gctcatgatg cggcttctcg
    14221 ttcttcagtt ttttgtgcga cgcacaatct agcatagcgt ccgtacgacg tcaggatcat
    14281 tacgcccgat cggcgggaat tccgtggcag gccagcgccg caatcgcctc ggaatggctt
    14341 gccacccgct cggcacgaag gccggtggcg cgggcaccgg cgacattggc cggcaggtcg
    14401 tcgaggaaca ggatatcggc ggccggcagg ccaattcgct ggaccacggc ggcgaaagcc
    14461 gcgccgtccg gtttgcgtgc gccaatctgg tgcgatacga agcattcgcg gaaatgcccg
    14521 agcagcggcg cgcaactctg ctgccagacg gcgtggtggg tcgcattggt gttgctgaac
    14581 aaaaagagcg gcatttcgcc ggcgagttcc tcgaccagcc gcgccatctc cgggcggacc
    14641 tgtccgagca tggccagcca gccggcgcgc cagctgtcgg cgtcgagcgt gatcgagagc
    14701 tgtttggtca gcgccgcgaa gaaatcgggc gcatcgcagt ggccgcactc gaaggcgcgg
    14761 taggtctcgt cttcgcgcaa gcgtccgcgc agttcatcgg cggggagtct gccgctcgga
    14821 tcggcggcgg cagcccaatg cgccagcgtg cgatcgagat ggacgtcgag gaggacgcca
    14881 ccgagatcga agaggacagc gcgcgggcgc atcgccggtc gcggctcagt gcagccgatg
    14941 ctcggcggcc tcgaggaact cgagtgccat cgcgcgatcg agccggtatt cgcgattgca
    15001 gatgtcgtcg cggatcacga tctcgccctg ttcgtcgagc agcgcctcga cctcgctgcg
    15061 gcccagcgcc gtcagcgtcg cgcgcacctt ttcccaatcc tccgggcagt ggtggacgac
    15121 ctcgcgcggc gtgaagacgc gtacggtttc ttcgtaaaac aggcgttgca gcagcgcttc
    15181 cggagcgagt tctagcagtt cctcaggctt tagcgtgtcg agcaggtggc cgatgcgggt
    15241 ccagccgtcg gggtcgcgcc ggtcggcgtc gggcattttc tgcacgaaga cgccgcaggc
    15301 cgctgtcgga ccggcggcga tgaccagtcg cgatgcctgc tgctcggact gccgcagata
    15361 gctttcgaag acctcgccta tcgtcgcgcc gtcgagcggg acgatgctct ggaagggcac
    15421 gcgcgaatct gggaggtcga tcgacatcag cagccgtccg tcgccgaaca tctcgggaac
    15481 cttggccgcc ggcatgtcgg ccggccagtc ggcgcgcgcc atgccgcgca ggcgcagcgc
    15541 ttcgtcggtg ctttcgcagt cgatcaccag caggccgacc gggccgttgc cgcgggcctg
    15601 aaaggtcagc cggccctcgt gcttgagctg ccctccgagg agaactgtaa tcgcggtcat
    15661 ctcgccgagc atctggctga tcgccggcgg gtagccgcgc tcggcgatca tcgtccgcca
    15721 ggcctcgccg aggtggacga tcgcgccgcg gatgtcgagc tgctcgaaga ggaagcgctg
    15781 gtagcggctg tcgctcatcg ccgtgcgcgg cctttaaccg cagtcagcgt cgccgcgaac
    15841 tgctcggggt cgaagccgag cagcacgccg ccgtcgtgtt cgatcaccgg gcggcgaacc
    15901 agcgtcgggt gggcgcgcat cagcgccagt gcgcggtcct cgtcgagatc gagccgctcg
    15961 gactcggcga gccggcgcca ggtcagcccg cggcggttga ccagcgcttc ccagccgagg
    16021 gcggcgatcc aatccggcag gcgctcggcg ccggccgttc ccttgtagtc gacgaagcgg
    16081 tactcaattc cctgtgcctc gaaccaggcg aacgtcttct tcatcgtgtc gcagttgcgg
    16141 atgccgtaga cggtaatcat gttttcagtc gggcgaatgc ggcggccatc gcgccggccg
    16201 ccagcggggg tgattgcgcg gcgcgtcgct ggggcgaacg cgtggagccg cggggcgccg
    16261 gcgcgggcgt tgtggtcggg tcgaccggcg ccttcaggcg catcgtgagc gcgatgcgct
    16321 ggcgcggcaa gtctaccgat acgaccttga cgcggacgat gtcgccgacc ttgaccactt
    16381 cgcgcgggtc gcggacgaag cggtcggcca gcgccgagac atggacgagg ccgtcctgat
    16441 ggactccgat gtcgacgaag gcgccgaagg cggcgacgtt ggtaacggtt ccttcaagct
    16501 gcatccccgg ctctaggtcg gaaagttcgc ggacgttctc gctgaacttc gcggtgcgga
    16561 actgcgggcg cggatcgcgg ccgggtttct cgagttcgcg caggatgtcg cgcacggtcg
    16621 gcacgccgaa acgctcgtct gcgtagcgct cggcttcgat tcccttgagc gtgcggctgt
    16681 cgccaagcag cgcgcgcagc gggcggccga gatcgacgag gatcttctcg acgagcgggt
    16741 aggactcggg atgcacgccc gagccgtcga gcgggttgtc gccgtcggtt atgcgcagga
    16801 atccggcggc ctgctcgaag gtcttgtcgc cgagccgggg tacggcaagc agtgccgtcc
    16861 ggctggggaa ggggccgttg gcgtcgcgcc agccaacgat gccttcggcg agcgccgggc
    16921 tcagtcccga gacccgtgcc agcagcggtg ccgaagcggt gttcacgtcg acgccgacgg
    16981 cgttgacgca gtcctcgacc acgtccccca gcgagcgggc cagcgccgtc tgcgaaacat
    17041 catgctggta ctggccgacg ccgatcgact tggggtcgat cttcaccagt tcggcgagcg
    17101 ggtcctgcag gcggcgggcg atcgacaccg cgccgcgcag cgagacatcg agttgcggga
    17161 actcgcgggc ggccagctcg gaggccgaat agaccgaggc gccagcctcg gagacgacca
    17221 ccggtgtcag cttcagctcg ggatggcggg caatcaggtc ggcgaccagt ttttcggttt
    17281 cgcggctggc cgtgccgttg ccgatcgcca ccagctcgat gtgctgcgcg gcggcgatgg
    17341 cggccaggcg agcgatgctg ccgtcccagt cgcggcgcgg ttcgtgcgga tagactgtgt
    17401 cggtggcgac gagcttgccg gtgccgtcga ccgcagcgac cttgaccccg gtgcggatcc
    17461 ccgggtcgag gcccagcgtc ggccgtctcc cggccggcgc ggcgagcagc agatcctgca
    17521 ggttcttgcc gaagacccgg atcgcctcgc gctcggcgcg ttcgcgcagc gcccccatca
    17581 gttccatctc gagatgcagt gccagcttga ctttccaggt ccagcgcgcc gtgtcgcgca
    17641 gccaaacgtc ggccggccgg ccctcgttgc ggattccggt gtgggcggcg acgcgctgct
    17701 cgcaggggtt gagctcgcgc gggtcgatgt cgtcggcgag gtcgctgtcg agcgccaggg
    17761 agacctgcag cagcccttcg tttcgcgcac gcagcagggc cagcgcgcgg tgcgacggga
    17821 cggtggtcca gtgctcgcgg aagtcgtggt agtcgcggta cttcgcggcc tcctgttcct
    17881 tgccggcgac tagtctgccg cagagtatcg cgtgctcgga aaagtaggcg cggattccgg
    17941 cgaccagcgt cgcgtcttca gcgaatcgtt ccatgagtat ctgccgcgcg ccgtcgagca
    18001 ctgccttggt gtcggcgaag cctgcctcgg cattgatgaa gccagctgcc gccgcttcgg
    18061 gaaccgtcat cgggtcgtcg atcagcaggt cggccagcgg ctcgattccc gcctcgcggg
    18121 cgatctgcgc cttggtccgc cgtttctgct tgtagggcag gtagaggtct tcgagcgcct
    18181 gcttggtctc ggcgccgagc aaggcggcac gcagggcgtc gctgagcttg ccctgttcgg
    18241 caacgctgtc gaggatcacc gcgcgccgtt cctcgaggcc gcgcaggtat tcaaggcgct
    18301 cggcgagcgt tctcagctgc gtgtcgtcga ggccgccggt ggcttccttg cggtagcggg
    18361 cgatgaaggg gacggtcgcg ccctcgtcga gcagccgggc ggcggcggtg acctgggcga
    18421 cgttgacgtc cagctgctgg gcaatgcgca gttcgatgga tgggagcatg gcgattcggg
    18481 aaaaggaatg cgggccacgg cgcggggcgc cgtggcggtg tcgatcaggc gggcgggaag
    18541 aagttgctca ggcggggacg ctgaggtcct tcagcgtgac gcgcggttcg atccgcggca
    18601 tcaccttctg caggccgcgg cgcgagcggg tgttgatcag caacggtgcc tgcaggttgg
    18661 cgcggatcgg cgcgccggca tcttcctgct tgtagagcac gagcatcagc gcaatgtcgg
    18721 tggggtctgc ggcctggagc aacgccgcct cgtcgtcgtt gagttcgagc tcgtagtgca
    18781 ggccgtaggc gccgggatcg gcaatctgca gcgcgat
//
LOCUS       DATDAA010000224         6527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_261068,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000224 DATDAA010000000
VERSION     DATDAA010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261068"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..906
                     /locus_tag="VIS73_07135"
     CDS             325..906
                     /locus_tag="VIS73_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEY5762965.1"
                     /translation="MFQIRIHGRGGQGVVTGAEMLSIAAFLEGKHAQAFPSFGSERTG
                     APVVAFCRIDTKEIRLREPIMAPDAIIIQDPTLLNQVDVFQGLKSDGYVLINSGKSFE
                     ELGIGEVAKKLRADRQVCVPATDIAMKHLGRPMPNAVLLGAFAGLSGVISLESVASAI
                     RDKFSGKVAEGNINAATAAFEFVKEKLSALEAA"
     gene            906..2156
                     /locus_tag="VIS73_07140"
     CDS             906..2156
                     /locus_tag="VIS73_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5762966.1"
                     /translation="MLKQIEGSHGVAEAIALCRPEVICAYPISPQTHIVEGLGEMVKT
                     GDLKAEYLNVESEFAAMSVAIGSSAAGARTYTATASQGLLFMVEAVYNASGLGLPIVM
                     TVANRAIGAPINIWNDHTDSMSQRDCGWIQLFAETNQEAMDLHIQAFKLAEELSLPVM
                     VCMDGFILTHAYERLDVPTQEQVDEFLPPFEPRQVLDPASPVSIGAMVGPEAFTEVRY
                     LAHVKQMQALDRIPQLAEEFKGVFGREAGGLVRPYKMDGAETIVIALGSVLGTIKDTV
                     DEMQAAGHKIGVLGITSFRPFPLEAIRAALKGAKRIVVLEKCFAVGVGGIVATNVRMA
                     LDGTGQEVLTVVAGLGGRSITKDSLARTFKEAIDGKLGAMTFMDLDHGIVNRVLEHEA
                     KVRRSGPIAENVLRDVGTVASKIA"
     gene            2168..3166
                     /locus_tag="VIS73_07145"
     CDS             2168..3166
                     /locus_tag="VIS73_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5762967.1"
                     /translation="MSFQPVRFYQTGTFTVGNRLLDERSVQATQGRDNSLNSGHRACQ
                     GCGEALGARYAIDAAMAASNRQLIAANATGCLEVFSTPYPETSWQMPWIHSLFGNAPA
                     VASGVAAAMRIKGKSDTRVVAQGGDGSTTDIGFGCLSGMFERNDDVLYICYDNEAYMN
                     TGVQRSSATPPAVRTATTMPVGPEPGNVFGQGKYVPGIAMAHGIPYVATATVADLRDL
                     EYKVKKAINIRGAKYIHIFVPCPLGWGAASHDTIKLARMAKECGLFPVFEAEFGEVTG
                     VSKIRNRIPVVDYMKPQRRYAHLFGKNPAIDTIARIQAKADRNIRKFGLIDEEGVN"
     gene            3166..4800
                     /locus_tag="VIS73_07150"
     CDS             3166..4800
                     /locus_tag="VIS73_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY5762968.1"
                     /translation="MSTVMNKPFAITLDVGSSLANKTGSWRTARPVYLDRLPPCNNQC
                     PAGENIQSWLFLAEEGKYEQAWRKIMEENPFPAVMGRVCYHTCEGACNRGKVDLPVGI
                     NSVERFLGDQALKYGWTVQPGKPTGKKVMVIGAGPGGLAAAYHLARLGHTVTVFEANP
                     KAGGMIRYGIPKYRMPREKLNAEIARIAGLGVEIKLGTKVADVAKTMKDGKFDACFLA
                     VGLQNGRKVDIPQDGNLPVLDAVTVLRAVELEEPVALNGRVVVYGGGNTAIDVARSAI
                     RLGALSATVVVRESREQMPAHHAEISEGIEEGITVVDLRQINQITGKTLTLEVMVPGE
                     GRELVRTGQTETIEADVVVQALGQTGDFTPLSGLSGIVVQDGAVQVGTNLMTGAVGVF
                     AGGDMIPSQRNVTAAIGLGKKAARNIDAFLRGTQYAPKPKAEVADVAKMNTWYYSDAP
                     RTIRPMLDVVRRTTGFAEVVGNLDEDNALFEARRCMSCGNCFECDNCYGVCPDNAVIK
                     LGPGNRFKFNYDYCKGCGICVSECPCGAIKMVPEEI"
     gene            5120..6124
                     /locus_tag="VIS73_07155"
     CDS             5120..6124
                     /locus_tag="VIS73_07155"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY5762969.1"
                     /translation="MDSLSQLALGSVIGVAVMGRRTAVWKAALWGGIAGTLPDLDALI
                     DYGDPLRNMTLHRAESHSLFYLTLFSPALALAASKLHGQLLYFKRWWLALWLALVTHP
                     CLDWMTVYGTQLGIPFVNTPFGLGSIFIIDPIYTLPLLVGVAAALIARSDRGLAWNRW
                     GLSLSCAYLLWTAGAQWRVEQIAERSLAQAGIEARQTLATPSPFNTVLWRIVAMTSDG
                     YVEGFYSFLDDDTEIRFNQHNSGRMLVPELAHIPGVERMRWFTRGFYSLSERDGRALV
                     TDLRMGQEPNYVFAFAVGQRGSGFIEHTPIAVGQRGDIGRGLAWVWQRLLGNDVPPPR
                     "
     gene            6153..>6527
                     /locus_tag="VIS73_07160"
     CDS             6153..>6527
                     /locus_tag="VIS73_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY5762970.1"
                     /translation="MAIETLIDGRVRNLGGFSVRRILPAGRQRMVGPFIFFDHIGPAD
                     LPPGDGIDVRPHPHIALATVTYLFSGALLHRDSVGSMQEVRPGDVNWMTAGHGIVHSE
                     RTPAAERSAGMRIHGIQSWVALP"
BASE COUNT         1200 a         2181 c         1972 g         1174 t
ORIGIN      
        1 atcctgttct gccgcatgcc gcgggtgact cacagttgat ctccgttcct cgttacatta
       61 gcgagcgcca agcctatcga tgttggcatc cgggtggcgc agcttggcga ctttcattga
      121 tctcgagggg tttaatccgc cgcttcggcc cgattgacaa ctaagggtaa tccgcaatag
      181 acttttcgcc atgcggtgct acatttcgta tagcgcaata caaaaccaaa agagccgcgc
      241 ccgccgtggc cgtgattttc cgccgtccgc ttggtgcagg ccgctgtgga cgattgatga
      301 aacgatccgt tggaggaaaa cggtatgttc cagattcgca tccacggccg cggaggccaa
      361 ggcgtcgtca ccggcgccga aatgctctcc atagccgcct ttctcgaagg caagcacgcg
      421 caggccttcc ccagcttcgg ttcggagcgc accggcgccc cggtcgtcgc cttctgccgc
      481 atcgacacca aggagatccg cctgcgcgag ccgatcatgg cgcccgacgc gatcatcatc
      541 caggacccga ccctgctcaa tcaggtcgac gtgttccagg gcctcaagtc cgacggctac
      601 gtgctgatca actcgggcaa gagcttcgag gaactcggca tcggcgaagt cgccaagaaa
      661 ctgcgcgctg atcgtcaggt ctgcgtaccg gccactgata tcgccatgaa acacctcggc
      721 cgcccgatgc ccaacgccgt gctgctcggc gccttcgccg gtctctcggg tgtcatctcg
      781 ctggaatcgg tcgcctcggc cattcgcgac aagttctcgg gcaaggttgc cgaaggcaat
      841 atcaatgccg cgacggccgc gttcgaattc gtcaaggaaa aactctctgc actggaggcc
      901 gcgtaatgtt gaagcaaatc gaaggttcgc acggcgttgc cgaagcgatc gcgctgtgcc
      961 gccccgaagt catctgcgcc tacccgatca gcccgcagac ccacatcgtc gaaggcctcg
     1021 gcgagatggt gaaaaccggt gatcttaagg ccgagtacct caacgtcgaa tccgagttcg
     1081 ccgcgatgtc ggtcgccatc ggatcgagcg ccgccggcgc ccgcacctac acggccaccg
     1141 cctcgcaggg cctgctgttc atggtcgagg ccgtctacaa cgcctcgggc ctcggcctgc
     1201 ctatcgtcat gaccgttgcc aaccgggcaa tcggtgcgcc aatcaacatc tggaacgacc
     1261 acaccgactc gatgagccag cgcgactgcg gttggattca gctgtttgcc gagaccaacc
     1321 aggaagcgat ggacctgcac attcaggcct tcaagctcgc cgaggaactc tcgctaccgg
     1381 tcatggtgtg catggacggt ttcattctca cccacgccta tgagcgtctc gacgtgccaa
     1441 cgcaggagca ggtcgatgaa ttcctcccgc ccttcgagcc gcgtcaggtc ctcgacccgg
     1501 cgtcgccggt ttcgatcggc gccatggtcg gcccggaagc ttttaccgaa gtgcgctacc
     1561 tcgcccacgt caagcagatg caggcgctcg accggattcc ccagctcgcc gaggaattca
     1621 agggcgtctt cggccgcgag gccggtggtc tggttcgccc atacaagatg gacggcgccg
     1681 aaaccatcgt catcgcactc ggttcggtac tcggcacgat caaggacacc gtcgacgaaa
     1741 tgcaggcggc gggccacaag atcggcgtgc tcgggatcac ctcgttccgt cccttcccgc
     1801 tcgaagcgat tcgcgcggca ctcaagggcg ccaagcgcat cgtcgtcctc gagaaatgct
     1861 tcgcagtcgg cgtcggcggc atcgtcgcca ccaacgtgcg catggcgctc gacggcaccg
     1921 gtcaggaagt cctgaccgtc gtcgccggcc tcggcggtcg ttcgatcacc aaggacagcc
     1981 tcgcacgcac cttcaaggaa gcgatcgacg gcaagctcgg ggcgatgacc ttcatggatc
     2041 tcgaccacgg catcgtcaat cgcgtgcttg agcacgaagc caaggtccgt cgttcgggcc
     2101 cgatcgccga aaacgtcctg cgcgacgtcg gcaccgtcgc ctcgaagatc gcgtaaggag
     2161 aaaccacatg tccttccaac ccgtacgttt ctaccagacc ggcaccttca ccgtcggcaa
     2221 ccgcctgctc gacgagcgct cggtgcaggc cacccagggc cgtgacaatt cgctgaactc
     2281 tggccaccgc gcctgtcagg gctgcggcga agcgctcggc gcccgctacg cgatcgacgc
     2341 tgcgatggct gcgtcgaacc gccagctgat tgccgccaac gccaccggct gcctcgaagt
     2401 gttctcgacg ccttaccccg agacttcctg gcagatgccg tggatccact cgctgttcgg
     2461 gaacgccccg gccgtcgctt ccggcgtcgc cgcggcgatg cgcatcaagg gcaagtccga
     2521 cacccgcgtc gtcgcccagg gcggcgacgg ttcgaccacc gacatcggct tcggctgcct
     2581 gtcgggcatg ttcgagcgca atgacgacgt gctctacatc tgctacgaca acgaggccta
     2641 catgaacacc ggcgtccagc gctcctcggc gaccccgccg gcggtgcgca cggcgacgac
     2701 gatgccggtc ggccccgagc caggcaacgt ctttggccag ggcaagtacg tgccgggcat
     2761 tgccatggcg cacggcattc cctacgtcgc caccgccacg gttgccgacc tgcgcgacct
     2821 cgaatacaag gtcaagaagg cgatcaacat ccgcggcgcc aagtacatcc acatcttcgt
     2881 gccctgcccg ctcggctggg gtgccgcctc gcacgacacg atcaagctag cgcgcatggc
     2941 caaggaatgc ggacttttcc cggtgttcga agccgagttc ggcgaggtca ccggcgtctc
     3001 gaagatccgc aaccggatcc cggtggtcga ctacatgaag ccgcagcggc gctacgcgca
     3061 cctgtttggc aaaaaccccg ccattgacac aatcgcccgc atccaggcga aggccgaccg
     3121 caatatccgc aagttcggtc tgatcgacga agaaggagtc aactgatgag taccgtcatg
     3181 aacaagccgt tcgcgatcac cctcgacgtc ggttcctcgc tcgccaacaa gaccggatcg
     3241 tggcgcaccg cccgcccggt ctacctcgac cggctgccgc cctgcaacaa ccagtgcccg
     3301 gccggcgaga acatccagag ctggctcttc ctcgccgagg aaggcaagta cgagcaggcc
     3361 tggcgcaaga tcatggaaga gaaccccttc ccggccgtca tgggccgcgt ctgctatcac
     3421 acctgcgagg gcgcctgtaa ccgcggcaag gtcgacctgc cggtcggtat caactcggtc
     3481 gagcgcttcc tcggtgacca ggccctgaaa tatggctgga cggtccagcc gggcaagccc
     3541 acgggcaaga aggtcatggt tatcggcgcc ggccccggcg gtctcgccgc cgcctatcac
     3601 ctggcgcgcc ttggccacac ggtcacggtt ttcgaagcca acccgaaggc cggcggcatg
     3661 atccgctacg gcattcccaa gtaccgcatg ccacgcgaaa aactcaacgc cgaaatcgcc
     3721 cgcatcgccg gcctcggtgt cgagatcaag ctcggcacca aggtcgccga cgtcgcgaag
     3781 acgatgaagg acggcaagtt cgacgcctgc ttcctcgccg ttggtctgca gaacggccgc
     3841 aaggtcgaca tcccgcagga cggcaatctg cccgtgctcg acgcggtgac ggtgctgcgc
     3901 gccgtcgaac tcgaagagcc ggtcgccctg aatggccgcg tcgttgttta cggcggcggc
     3961 aacacggcaa tcgacgtcgc ccgttctgcg atccgcctcg gcgcgctgtc ggcgacggtc
     4021 gtcgtccgcg agtcgcgaga gcagatgccg gcgcaccacg ccgagatcag cgaaggcata
     4081 gaggaaggca tcaccgtcgt cgacctgcgc cagatcaacc agatcaccgg caagaccctg
     4141 acgctggaag taatggtccc gggtgaaggc cgcgaactgg tgcgcacggg ccagaccgag
     4201 accatcgaag ccgacgtggt cgtccaggcg ctcggccaga ccggcgactt cacgccgctc
     4261 tcgggcctgt ccggcatcgt cgtgcaggac ggcgcggtgc aggtcggcac caacctgatg
     4321 accggtgcgg tcggcgtctt cgccggcggc gacatgattc cgtcgcagcg caacgtcacc
     4381 gcagccatcg gactcggcaa gaaagcggcg cgcaacatcg acgccttcct gcgtggaacc
     4441 cagtacgcgc cgaagccaaa ggccgaggtc gccgatgtgg ccaagatgaa cacctggtac
     4501 tactcggatg ccccgcggac gatccgcccg atgctcgatg tcgtgcgccg taccaccggc
     4561 tttgccgagg tcgtaggcaa cctcgacgag gacaacgcgc tgttcgaggc ccgtcgctgc
     4621 atgagctgcg gcaactgctt cgagtgcgac aactgttacg gcgtctgtcc ggataacgcg
     4681 gtaatcaagc tcggccccgg caatcgcttc aagttcaact acgactactg caagggctgc
     4741 ggcatctgcg tgtccgagtg tccctgcggc gcgatcaaga tggttccgga agagatctga
     4801 gctcagctcg acggtaatca gatccctgaa cgcccggcgg cgccagcccc gggcgttttt
     4861 ttgtgtcgtt agggcggccg accttatcgg aagtggcgtt ggcttgagct accggaaaca
     4921 ccgaagctgc cgtcgcggtc taagccgtca gcctgcccgt ggccattcgt tcgctacgat
     4981 ctgaatcgtt ccccactgtt cacacgacga agccgcgcca caccttcgcc ggtgcgatcg
     5041 caccggaact tgcgcccgac tcctcggtcg attcgctttc tcctccagcg cgcggaaaag
     5101 ccgctcgcaa ggcaaggtcg tggattccct ctcgcaactg gcacttgggt cagttatcgg
     5161 cgtcgccgtg atggggcggc gaaccgccgt ttggaaggcg gcgctttggg gcggtatcgc
     5221 cggcacgctg cccgatctgg acgcgctgat cgactacggc gacccgctgc gcaatatgac
     5281 gctccaccgg gccgagagtc actcgctctt ctatctgacg ctgttctcac ccgccctcgc
     5341 gctggcagcg tcgaaactgc atggccagtt gctgtacttc aagcgctggt ggctggcctt
     5401 gtggctggcc ctagtcacgc atccgtgtct cgactggatg acagtctacg gcacgcaatt
     5461 gggcattcca ttcgtcaaca cgcctttcgg gcttggcagc atattcatca tcgacccgat
     5521 ctacacgctg ccgctgctgg tgggagttgc ggcggcgttg atcgcgcgtt ccgaccgcgg
     5581 gcttgcttgg aatcgctggg ggctcagtct cagttgcgcc tatctgctct ggaccgccgg
     5641 tgcgcagtgg cgtgtcgagc agatcgccga gcgatcactc gcccaggcag gaatcgaggc
     5701 ccgacagact ctcgccacgc cctcgccgtt caacacggtt ctgtggcgga tcgtcgcgat
     5761 gacaagcgac ggctatgtcg agggctttta ctcgtttctg gatgacgaca ccgaaatccg
     5821 gttcaatcag cataattccg gccggatgct cgtccccgaa ctggcccata ttccaggcgt
     5881 cgaacgcatg cgctggttta cccgtggctt ttattcactc agtgagcgcg acggacgtgc
     5941 attggtcacc gatctgcgga tgggacagga accgaactac gtgttcgcgt ttgccgttgg
     6001 acaacgcggc agcggattta tcgaacatac gccgatcgcg gtaggccagc gcggcgatat
     6061 cggtcgcgga ctcgcgtggg tttggcagcg gcttcttggc aacgatgttc caccgccgcg
     6121 ctgaccgagg tgtttgacta acaggaggtt ccatggcgat cgaaacactg attgacggac
     6181 gagtccgcaa tctgggcggc ttttccgttc ggcgcatcct gccggccggt agacagagaa
     6241 tggtggggcc gttcatcttt ttcgaccaca tcggaccggc cgatctgcca ccgggcgacg
     6301 gtatcgacgt gcgcccacat ccgcacatcg cactggcaac cgttacctac ctcttctcgg
     6361 gcgccttgtt gcatcgagac agcgtcggca gcatgcagga ggtccgcccg ggcgacgtca
     6421 attggatgac agccgggcat ggcatcgtcc actccgagcg gacgccggcg gcggaacgtt
     6481 ctgccggcat gcgaatacac ggcattcagt cgtgggtagc cctgccg
//
LOCUS       DATDAA010000225         2134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_471123,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000225 DATDAA010000000
VERSION     DATDAA010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471123"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VIS73_07165"
     CDS             <1..428
                     /locus_tag="VIS73_07165"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY5762971.1"
                     /translation="CVGEIELNRRGLNAGPPGARLPSNMAPSVARRDGAVIAVGSPGA
                     DRITTALQQFLINYLQLGMPLEDAIAHPRVHVDTSGEIINLKAEPGLDLPELDLPVSV
                     FPELVMYFGGVGAAVFDSVTGFDVGADPRREGGVFIPDA"
     gene            521..>2134
                     /locus_tag="VIS73_07170"
     CDS             521..>2134
                     /locus_tag="VIS73_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5762972.1"
                     /translation="MDSNPDARLLIDLSGALWLIPAGGGEAERLAENHGEVRRPRWSP
                     DGARVVYAANANGARGLRLHDISTGAAQPLGENAWLDFHPAWHPGGERIVFASDRRDT
                     GLDIWEVDVKTGLEWRLTHRPGDETEPAWSASGRDLVYVHRVQDSWSLVLRRHGQAEE
                     VLVTGNERLAGPSWRPDGSLVVFWRETEEGLALDMVILSEPRLVRRYMDGEDYTAAPV
                     SWLDRHRMFYSAGGLIRQRLFNAWSSKTVPFRATLETEAEVVVEPVRRNLPRIDEPTG
                     IRVIRAQRLFDGIGGGYLENLDILIDGGRILAVGPRAEHPGAIVIDMGDLTILPGLVD
                     VSTRLPENADEAIGPLLLAAGVTTMVADNEQAEHLNAVWSGKELPGPRLLPAGDWPVP
                     SFSGLADSQTPGLDTLLASRAAGLTGFDQPVARRFAEPPTIEHGVTDMVLGSLGNGLP
                     AGIASHAELRALQAAGLKPEQALRAAGVNAAAALGVDPQLGRIATGAVADLILVDGDP
                     LMSVDDALQVVAVVRNGRFYSVAGLIERAS"
BASE COUNT          353 a          702 c          711 g          368 t
ORIGIN      
        1 actgtgtcgg cgaaatcgag ctcaaccggc gtggtctcaa cgccggcccc ccgggagcaa
       61 gactgccatc caacatggcg cccagcgtgg cgcgtcgtga tggtgcagtc atcgccgtcg
      121 gctcaccggg agcagatcgc ataacgaccg cgctccagca gttcctgatc aattacctgc
      181 agctcggcat gcctctcgag gacgctatcg cgcacccgcg agttcacgtc gacaccagcg
      241 gggaaattat caacctgaaa gcggaacccg ggctggacct gccggagctg gatctccccg
      301 tatcggtctt cccggaactt gtcatgtact tcggcggcgt cggcgcagcc gtatttgatt
      361 ccgtaactgg tttcgatgtg ggcgccgacc cacgccgtga aggcggagtc ttcattcccg
      421 acgcatgacg acccggcgcg ccattattcc tctttggtgt ctgatcgctg cggccgcaag
      481 tccggcggaa gaagtcgtta cgcgcgggag caatcttgcc gtggactcaa accccgacgc
      541 gcggctgctc atagatcttt caggcgcgct atggttgatt cctgccggcg gcggcgaagc
      601 cgaacgtctc gcggaaaacc acggcgaggt gcggcggccc cgctggtcgc ccgatggcgc
      661 ccgcgtcgtg tacgccgcga atgccaacgg cgcgcgcgga ctgcgactcc acgatatcag
      721 caccggggct gcgcagccgc tgggcgaaaa tgcctggctc gacttccatc cggcatggca
      781 tccgggcggg gaacgcatcg tgtttgcctc ggaccggcgg gatacgggac tcgacatctg
      841 ggaagtggat gtgaagaccg gactcgaatg gcgcctcacc catcggccgg gcgacgagac
      901 cgagccggca tggtctgcca gcgggcgcga tctcgtctat gtgcatcgtg tgcaggacag
      961 ctggtccctg gtgttgcggc ggcacgggca ggccgaagag gtactcgtca ccggtaacga
     1021 gcgacttgcc ggcccgtcgt ggcggccgga cggtagcctc gtggttttct ggcgcgagac
     1081 cgaggaaggc ctggctctcg acatggtgat cctctctgag ccgcgcctcg tacgccgtta
     1141 catggacggc gaagactaca cggcggcacc ggttagctgg ctcgaccgcc accggatgtt
     1201 ctactccgcc ggcggactca ttcggcaacg cctgttcaac gcgtggtctt cgaaaaccgt
     1261 gccgtttcga gccacgctgg agacggaggc cgaagtggtc gtcgaaccag tgcggcgcaa
     1321 cctgccgcgc atcgacgagc cgacaggcat acgggtgatt cgcgcgcagc ggctgttcga
     1381 cggaatcggc ggcggctacc tggagaacct cgatatcctg atcgacggtg gtcgcatcct
     1441 ggcggtcggg ccccgagccg agcatcccgg cgcaatcgtt atcgatatgg gcgatctcac
     1501 gatcctgccc gggctcgtcg acgtcagcac gagactcccc gaaaacgccg atgaagcaat
     1561 cgggccgctg ttgctcgccg cgggcgttac gacgatggtt gcagataacg agcaggcgga
     1621 gcacctgaac gccgtgtggt ccggtaagga gttgccgggc ccgagattgc tgccggccgg
     1681 cgactggccg gtcccgtcct tctccgggct cgccgacagt cagacgccgg gactcgacac
     1741 cttgctcgcc tcacgtgccg ccggcctgac cggattcgac cagcctgttg ctcgccgctt
     1801 cgccgagccg ccgacaatcg aacatggcgt caccgacatg gtgctcggca gtctcggcaa
     1861 cggcttgccc gcgggtatcg caagccatgc ggagttgcgc gcgctgcagg cggccgggct
     1921 gaaaccggag caggccctgc gtgccgctgg cgtcaacgcg gccgccgcac tcggcgtgga
     1981 cccgcagctc ggtcgcatcg ccacgggcgc ggttgccgac ctgatcctgg tcgacggcga
     2041 cccgctgatg tcggtcgacg acgcgctgca ggtggtcgct gtggttcgaa acgggcgctt
     2101 ctatagcgtc gccggcctca tagagcgcgc ctca
//
LOCUS       DATDAA010000226         9903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_16362,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000226 DATDAA010000000
VERSION     DATDAA010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9903
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16362"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VIS73_07175"
     CDS             complement(<1..396)
                     /locus_tag="VIS73_07175"
                     /inference="COORDINATES: protein motif:HMM:NF023282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HEY5762973.1"
                     /translation="MTQTTRISFRRRLLASALLLAFAQPSFADAERQALDEALRQDLF
                     TADSRVSVGLGNLSGEHRRYNLYRGFADDGFYALLDADLDQRNADGTSIRLTGRNLGL
                     DTRSLRVDGERQGAWRLGFDYRQFIRREPL"
     gene            complement(407..1297)
                     /locus_tag="VIS73_07180"
     CDS             complement(407..1297)
                     /locus_tag="VIS73_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HEY5762974.1"
                     /translation="MLPSLRFVVITTVIALLPLGKAIAASEKSLDEAADILMTGDAVC
                     TRCHNDVSDGIVDDYPVLSIGRTRHGVAADQRTPTCVACHGQSQDHIDNKREPGSGKR
                     GQRPAPDFPFGKNAAATGKAHNDSCLACHAGDKLMHWNGSIHARRDVPCSDCHKMHVA
                     QDPAMTREKLPEMCMSCHKEKRAQFARPNHHPVPEGQMVCTDCHNPHGSAGPKLMVKD
                     SVNDTCYSCHMDYRGPFLCQQCHEPANHRGNFPSLNGANANSPGSGGLTQARGCVNCH
                     TNLHGGNNPTNALNTRSFRR"
     gene            complement(1365..1694)
                     /locus_tag="VIS73_07185"
     CDS             complement(1365..1694)
                     /locus_tag="VIS73_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5762975.1"
                     /translation="MSTSQLLVTVFLAALATSAFAKVDEEAAIALAKKSDCFKCHSVE
                     TKKEGPAYREVAKKYKGKAEAEDKLFTHVTTAPIVKVDGVEEEHQIVKTRKKDAIENL
                     IRWILSR"
     gene            1862..2986
                     /locus_tag="VIS73_07190"
     CDS             1862..2986
                     /locus_tag="VIS73_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY5762976.1"
                     /translation="MAIKRVVMGEILPAELEIGVPLPYTVYDENHTLLLHKGAVLGTQ
                     RQLDALLERGLYRVDDVGGVAEAPLPENPLVRLDRLCRELNTLIAAPARAEGRFENGI
                     VSIATRLGLLCDEYPDATLAGMLHDRQTRYAVRHMAHCAVVSHLLADAVGLVPSERLA
                     VVCAALTMNLSIVNLQNELNGSVLRSVTENERQQILAHPERTVAALRSHGVADEPWLD
                     YVLQHHEAYDGSGYPFGRSGAGIELGARIVGLVDRYIARLRHRADREGKLPNESLRDI
                     FVSRGAEVDPQLAQFLVKTVGIYPPGLVVELENGEVGVVIRRTDAAHAPDVQVLVARE
                     RLLAQPEGRLTDQAPFRIRSGLMADSMRYRFDPLRFWQEA"
     gene            complement(2993..3415)
                     /locus_tag="VIS73_07195"
     CDS             complement(2993..3415)
                     /locus_tag="VIS73_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762977.1"
                     /translation="MHPTRRHRYALAALAAIGCAACSQQVDIATTQCDLNRGPCTFTL
                     DNGESGRIALSPRPLPLMKPIAIGIEAPVRTARLDFQGTTMDMGFNQATLRRDDQGRL
                     SGSALLPVCATGAMRWRVDLYLDDSPTATARFEFDTGR"
     gene            3414..4037
                     /locus_tag="VIS73_07200"
     CDS             3414..4037
                     /locus_tag="VIS73_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY5762978.1"
                     /translation="MSRIVLFNKPYGVVSQFTPEGRWRGLREVLPLHGLRVAGRLDAD
                     SEGLLVLTDDGALQTRIAEPRHKLAKTYWAQVEGIPSADALQRLACGVALADFVTQPC
                     LAESMDAPAGLWERDPPIRFRQSIPTAWVRLTLREGKNRQVRRMCAAVGHPVLRLIRY
                     AVGAATLDGLAPGAWIEGDTAMLGITKSGKRAIRRPRRNPEKNVRKK"
     gene            4034..4474
                     /locus_tag="VIS73_07205"
     CDS             4034..4474
                     /locus_tag="VIS73_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY5762979.1"
                     /translation="MIRRLLLLLVALALPAALLAEMPRIELRYGLYRINAEVAHTPAL
                     LQQGLMHRREMAAHRGMLFVFERDAAHCMWMRNTFIPLSVAFIDVQGKVINVADMTPH
                     DETPHCAAKPARYALEMNRGWFAARGVKPGAAISGIERAPPFSR"
     gene            complement(4484..5290)
                     /locus_tag="VIS73_07210"
     CDS             complement(4484..5290)
                     /locus_tag="VIS73_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5762980.1"
                     /translation="MTQQPALTDARLVVSTRVATLTFNRHDVRNALTGTALIDDIVAV
                     ANWINRAPEVSVLVMTGAGSAFSAGGNVKDMAERGGDFAGDVAEVAERYRHGIQQIPL
                     ALQGVEVPIIAAVNGPAIGAGFDLANMADIRIASTQAKFGETFLNLGIIPGDGGAWFM
                     QRLIGYQRAFELTLTGRVIDADEALRHGIVMEVVAADQLLPRTIELATRMAAQPPKAL
                     RLTKRLMKMAQRMELKDFLDLCASFQGMCHNEPEHLDAVNRFLAAQSKRS"
     gene            complement(5287..7461)
                     /locus_tag="VIS73_07215"
     CDS             complement(5287..7461)
                     /locus_tag="VIS73_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY5762981.1"
                     /translation="MTVTNTRSPLHDFLAPDSIAIVGASADPTKRGYKAMVGLIKDGY
                     AGKVYPINPKVDMILGVKTYPSITDVPGSIDLALLCTPSATVAGLVAECGRKGVKGAI
                     ILASGFKETGAEGAKLEQQVLDAARTGGVRLIGPNTSGMFNLHKKVNLLALDKVKAGD
                     IGFISQSGNMLLALVLEAEHNGNVGFSTYVGPGNQADIGFNDYLRYLGEDENTRVATL
                     YVEGFRDGRRFLRFAREVTAKKPVVVYKSGATEQGRKAAASHTGALAGSYNMTVDVLR
                     QVGVSVVQNSDEILPVAEGLGLLQQARGKRIAVVSDGGGQATIASDRLAEAGLELVEL
                     HEETRKRLSAILVPQASVINPVDVAGTSDAKPSVLADCLRIVAEDNNVDAVFLVGMFG
                     GYYQRFAETLLGDEMRGAETMIEIMREVGKPVVVFSLYAPIKPPALRRLREAGLPVYA
                     SIEQAVRVLAALGERGIYLAASEAKADIRSCKPLHDAQAIFAEAQNEGRDLFEFEAKN
                     IFRLHGVTIPEELIVHDAAELPAAVTQFGEQPLAMKIVSKDILHKSDAGGVKLNVTGP
                     DQAAKVYEKIMASCRAYDPAAAIRGIQLGPMARKGTEVIIGVIRDPIFGPVLMFGLGG
                     IFVEVLEDVAFRAIPLSRHDASTMVDQIKARKILRGVRGEAAVDREALVDLLLTVSAM
                     VEAYPQIDELDLNPIIAYENGYAIVDARVIVNRSLVTQGDDL"
     gene            complement(7464..8246)
                     /locus_tag="VIS73_07220"
     CDS             complement(7464..8246)
                     /locus_tag="VIS73_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY5762982.1"
                     /translation="MDAQTIVYSSTDGIAEIRLNRPQRLNAVTQQLYDELNDALGVAE
                     SDPDVRVVVLSGEGRAFCVGADLKEHKAGRTAFDRRQYLRGEQIVCKRLMLLSKPVVA
                     AVNGYALGAGAELAIASDFLLMAEGAQIGLPEISLGNFLGGGVTALLPRLVGLAKARE
                     LVFLGERIDGKEAVRIGLANRCFADASFRDDVRAFAKKVAGKAPFSMQLAKEQLNLSG
                     ERTLDAVLTAELEGMMFVGTTADWQEGIDAFAEKRSPVFKGR"
BASE COUNT         1660 a         2992 c         3362 g         1889 t
ORIGIN      
        1 cagcggttcg cggcgaatga actgccggta gtcgaagccg aggcgccagg caccctggcg
       61 ttcgccatcg acgcgcaggc tgcgcgtatc gaggccgagg ttgcggccgg taaggcggat
      121 cgatgtgccg tcggcgttgc gctgatcgag gtcggcgtcg agcagcgcgt agaagccgtc
      181 atcggcaaag ccgcggtaga ggttgtagcg gcggtgttcg cccgacaggt tgccgaggcc
      241 gaccgacacg cggctgtcgg cggtgaacag atcctggcgc agggcctcat cgagggcctg
      301 gcgctcggcg tcggcgaagg acggttgggc gaaggcgagc aacagcgccg aggcaaggag
      361 gcgccggcga aacgagatgc gagtcgtctg ggtcatggcg ggtctcctag cggcggaagc
      421 tgcgcgtgtt gagcgcattg gtcgggttgt tgccaccgtg cagattggtg tggcagttca
      481 cgcagccgcg cgcttgcgtc aatccgccgc tgcccggtga gttggcgttg gcgccgttga
      541 ggctcgggaa gttgccgcgg tggttcgccg gttcgtggca ctgttggcag aggaaggggc
      601 cgcggtaatc catgtggcag ctgtagcagg tgtcgttgac gctgtctttg accatcagct
      661 tcggacccgc cgagccgtgc ggattgtggc aatcggtgca caccatctgg ccttcgggca
      721 ccgggtggtg gttggggcgg gcgaactgcg cacgtttctc cttgtggcag gacatgcaca
      781 tttccgggag cttttcgcgc gtcatcgccg gatcctgcgc gacgtgcatc ttgtggcagt
      841 cggaacaggg cacgtcgcgg cgggcgtgga tgctgccgtt ccagtgcatc agcttgtcac
      901 cggcgtggca ggcgaggcag ctgtcattgt gggccttgcc ggtcgctgcg gcgttcttgc
      961 cgaaggggaa gtcgggtgcc ggtcgctgac cgcgcttgcc cgaacccggt tcgcgcttgt
     1021 tgtcgatgtg gtcctgactc tgaccgtgac aggcgacgca ggtcggcgtc cgctggtcgg
     1081 cggcgacccc gtggcgggtc cggccaatcg acaggaccgg atagtcgtcg acgatgccgt
     1141 ccgagacgtc gttgtgacag cgggtgcaga cggcatcgcc ggtcattagg atgtcggcgg
     1201 cctcatcaag cgacttttcg gaggcggcaa tggccttgcc aagcggcaag agcgcgatga
     1261 cggtcgtgat gaccacgaac cggagcgatg gcagcattta cttctccctt tttgtgtctt
     1321 ttgtggctgt ctgtctttgc agcttggcgc gttgtcatag tttatcagcg cgacaggatc
     1381 cagcgaatca ggttttcgat cgcgtccttc ttgcgggtct tgacgatctg gtgctcttcc
     1441 tcgacgccgt caaccttgac gatcggcgcc gtggtcacgt gcgtgaacag cttgtcttcg
     1501 gcctcggcct tgcccttgta tttcttggcg acctcgcggt aggccgggcc ttccttcttg
     1561 gtttcgaccg aatggcactt gaagcagtcg ctcttcttgg ccagcgcgat ggccgcttct
     1621 tcgtcgactt tggcgaaggc gctggtggcg agcgcagcaa gaaacacggt gacaagcagt
     1681 tgactggtcg acattggtgt tgggggtagg agcgccctgc ggggctttgt gtttgcgggc
     1741 attgtagtcg tcttcggcgg gattacacga ccctcaacgc gtgaggccac aattgggttg
     1801 aagcgcctga tgacgtatgc ttcggctgca cggaagaacg acggcaaacg gagtctgggg
     1861 cgtggcgatc aagcgggtcg tgatgggcga aattctgccg gcggagctgg aaatcggcgt
     1921 gccgctgcca tataccgtct acgacgaaaa ccacaccttg ctcctgcaca agggcgccgt
     1981 gctcggcacg cagcgccagc tcgatgcgct gcttgagcgt gggctctacc gcgtcgacga
     2041 cgtcggcggc gtggccgagg cgccgctgcc ggagaaccca ctggtccggc tcgaccgtct
     2101 gtgccgcgaa ctcaatacgc tgattgccgc gccggcacgg gccgaaggcc ggttcgagaa
     2161 cggcatcgtg tcgatcgcga cgcgcctcgg gctgctctgc gacgagtacc cggacgcgac
     2221 cctggccggc atgctgcacg accggcagac ccgctatgca gtccgtcaca tggcgcattg
     2281 cgcggtggtc agccatctgc tggccgatgc cgttggccta gttccgagcg agcggctggc
     2341 cgtcgtctgc gcggcgttga cgatgaacct gtcgatcgtc aacctgcaga acgaactcaa
     2401 cggatcggtg ttgcgctcgg tcaccgagaa cgagcggcag cagattctcg cgcaccccga
     2461 gcggaccgtg gctgcgctgc gcagccatgg tgttgccgac gagccctggc tcgactacgt
     2521 gttgcagcat cacgaggcct acgatggcag cggctacccg tttggccggt cgggcgccgg
     2581 catcgaactc ggcgcccgta tcgtcgggct ggtcgatcgc tacatcgccc gcctgcggca
     2641 ccgtgccgac cgcgagggca agctgcccaa cgaaagcctt cgcgacatat tcgtcagtcg
     2701 cggtgccgag gtcgatccgc agctcgcgca gttcctggtc aagaccgtcg gcatctatcc
     2761 gccggggctc gtcgtcgaac tcgagaatgg tgaagtcggt gtcgtcattc gccggaccga
     2821 cgcggcacac gcgcccgacg ttcaggtgct ggtggcgcgc gagcggctgt tggcgcagcc
     2881 cgaagggcgg ctgaccgatc aggcgccgtt tcggattcgt agtgggctga tggccgactc
     2941 gatgcgctat cgcttcgatc cgctgcgctt ctggcaggaa gcctgagtgc cgctagcggc
     3001 cggtatcgaa ctcgaagcgg gcggtggctg tcggcgaatc gtcgaggtac agatcgacgc
     3061 gccagcgcat cgcgccggtc gcgcacaccg gcagcagggc ggacccgctg agtctgccct
     3121 ggtcgtcgcg ccgcagcgtc gcttggttga aacccatgtc catcgtcgtg ccctggaagt
     3181 cgagccgtgc cgtccggacc ggcgcctcga taccgatcgc aatcggtttc atcagcggca
     3241 gtggtcgcgg cgagagggca atgcggccgg attcgccatt gtccagcgtg aaggtgcagg
     3301 ggcctcgatt gaggtcgcat tgggttgtcg cgatgtcgac ctgctggctg caggcggcgc
     3361 agccgatggc cgccagtgcg gcgagggcgt agcgatggcg gcgggtcggg tgcatgtcgc
     3421 gaatcgtgct gtttaacaag ccctacggcg tggttagcca gtttacgccc gaggggcgct
     3481 ggcgcggcct gcgcgaggta ctgccgttgc atgggctgcg cgtcgcgggg cgactcgatg
     3541 ccgacagcga aggcctgctg gtgctgaccg acgatggcgc gctgcaaacc cgcatcgccg
     3601 aaccgcggca taaactggcg aagacctatt gggcgcaggt cgagggaatt ccttccgccg
     3661 acgcgctgca gcgtctcgcc tgcggtgtcg cgctggctga tttcgtgacg cagccctgtc
     3721 tggctgagtc gatggacgcg cctgccgggc tctgggagcg cgacccaccg attcgttttc
     3781 gccagtcgat tccgacggcc tgggtcaggc tgacgctgcg cgaaggaaag aaccggcagg
     3841 tccggcgcat gtgcgcagcg gtcggtcatc cggtgctgcg cttgatccgt tatgcggtgg
     3901 gggcggcaac gctcgacggg ctggcgcccg gcgcctggat cgagggcgat acggcgatgc
     3961 tcggcattac aaaaagcggt aaacgggcca ttcgccgccc acgccgcaac ccggaaaaga
     4021 acgtgaggaa gaaatgattc gtcgcctgct cttgctgttg gtcgcgctgg cactgccggc
     4081 cgccctgctc gccgaaatgc cgcgaatcga gttgcgctac ggcctttacc ggattaatgc
     4141 tgaggttgcc cacacaccgg cgctgctgca gcaggggctg atgcatcgtc gcgagatggc
     4201 ggcgcaccgc ggcatgctgt tcgtcttcga gcgcgacgcg gcgcactgca tgtggatgcg
     4261 caacaccttt attccgctct cggtcgcctt catcgatgtg cagggcaagg tcatcaacgt
     4321 cgccgacatg acgccgcacg acgaaacgcc gcattgcgcc gcgaagccgg cgcgctacgc
     4381 gctggagatg aaccgcggct ggttcgccgc gcgcggggtt aagccaggcg cggcgatcag
     4441 cggcatcgag cgggcgccgc cgttctcgcg ctagggcgag acttcagctg cgcttcgatt
     4501 gcgcagcaag aaagcgattg acggcgtcaa ggtgctcggg ctcgttgtgg cacatgccct
     4561 ggaagctcgc gcagagatcg aggaaatcct tcagctccat tcgttgcgcc atcttcatca
     4621 ggcgcttggt cagccgcagc gccttcggcg gttgcgcggc catgcgggtg gccagctcaa
     4681 tggtccgcgg caacagttgg tcggccgcga ctacttccat cacgatgccg tggcgaagcg
     4741 cttcgtcggc atcgatgacg cgtccggtga gcgtcagctc gaaggcacgc tggtagccga
     4801 tcagccgctg catgaaccag gcaccgccat caccgggaat gatgccgagg ttgagaaagg
     4861 tctcgccgaa ttttgcctgc gtcgaagcga tccggatgtc ggccatgttg gcgaggtcga
     4921 agccggcgcc gatcgccggc ccgttgaccg cggcgatgat cggcacttca acgccctgca
     4981 gcgcgagcgg aatctgctgg atgccatggc ggtagcgctc tgcgacctcg gccacgtcgc
     5041 cggcaaaatc gccgccgcgc tcggccatgt ccttgacgtt accgcccgcc gagaaggccg
     5101 agccggctcc ggtcattacc agcaccgaga cttccggggc gcggttgatc cagttggcga
     5161 cggcaacgat gtcgtcgatc agcgcggtgc cggtcagcgc attgcgtacg tcgtggcgat
     5221 tgaaggtcag cgtcgcaacg cgtgtgctga ccacgaggcg tgcatcggtg agtgcgggtt
     5281 gctgggtcac aggtcgtctc cctgggtgac aaggctgcga ttgacgatga cgcgggcgtc
     5341 gacgatcgcg tagccgtttt cgtaggcgat gatcggattg aggtcgagtt cgtcgatctg
     5401 cgggtaggcc tcgaccatcg ccgacacggt cagcagcagg tcaaccagcg cctcgcggtc
     5461 gaccgccgcc tcgccgcgga cgccgcgcag gatcttgcgc gccttgatct ggtcgaccat
     5521 cgtcgaagcg tcgtggcgtg acagcggaat ggcacggaag gccacatctt ccagtacctc
     5581 gacgaagatg ccgccaaggc cgaacatcag caccggcccg aagatcggat cgcggattac
     5641 gccgataatg acctcggttc ccttcctcgc catcggcccg agctggatgc cgcggatcgc
     5701 cgctgccgga tcgtaggcgc ggcacgacgc catgattttc tcgtagacct tcgccgcctg
     5761 gtctggaccg gtgacgttga gcttgacgcc gccggcatcc gacttgtgca ggatgtcctt
     5821 ggaaacgatc ttcatcgcca gcggctgttc gccgaactgg gtgacggcgg ccggaagctc
     5881 cgccgcgtca tgaacaatca attcctcggg gatggtcacg ccgtgcaggc ggaagatatt
     5941 tttcgcctcg aattcgaaga ggtcacgccc ctcgttctgc gcctcggcga aaattgcttg
     6001 ggcatcgtgc agtggcttgc aactgcggat gtcggccttg gcttcgctgg cagcaaggta
     6061 gatgccgcgc tcgccgaggg cggccagcac gcgcaccgct tgttcgatcg aggcgtagac
     6121 cggcaggccc gcttcgcgca gccggcgcag cgccggcggc ttgattggcg catagagcga
     6181 gaagacgacg accggcttgc cgacttcccg catgatctca atcatcgtct cggcgccgcg
     6241 catctcgtca ccgagcagtg tttcggcaaa gcgctggtag tagccgccga acatgcctac
     6301 gaggaacacc gcgtcgacgt tgttatcttc ggcgacgatg cgcaggcagt cggcaagtac
     6361 tgatggcttg gcgtcgctgg tgccggcgac gtcgactgga ttaatcaccg aggcctgcgg
     6421 aaccaggatc gccgacagcc gcttgcgcgt ttcctcgtgg agttcgacga gttccagccc
     6481 cgcctcggcg agccggtcgg aggcgatcgt tgcctggccg ccgccgtcgg aaaccacggc
     6541 aatccttttg ccgcgcgcct gctgcagcag gcccaggccc tctgccaccg gcaggatttc
     6601 atcggagttc tggaccacgc tgacaccgac ctggcgcagc acgtctaccg tcatgttgta
     6661 gctgccggct agcgcacccg tgtgcgaggc agcggccttg cgcccctgct cggtcgcgcc
     6721 tgacttgtag acgaccaccg gtttcttcgc cgttacctcg cgggcaaagc gcaagaagcg
     6781 gcggccgtcg cggaagcctt caacgtaaag cgtcgcgacg cgcgtgttct cgtcttcacc
     6841 gagatagcgg aggtagtcgt taaagccgat gtcggcctgg ttgccggggc cgacgtaggt
     6901 ggagaagcct acgttgccgt tgtgctcggc ctcgagtacg agcgcaagca gcatgttgcc
     6961 tgattgcgag atgaagccga tgtcgccggc cttgacctta tcgagcgcga gcaggttcac
     7021 tttcttgtgc aggttaaaca tgcccgaggt gttggggccg atcaggcgaa cgccgccggt
     7081 gcgcgccgca tcgagaacct gctgctcgag tttggcgccc tcggcaccgg tctccttgaa
     7141 gccgctggcg aggatgatcg cgcccttgac gcccttgcgg ccgcactcgg cgacgagccc
     7201 ggcaaccgtg gcagacggcg tgcagagcaa ggccagatca atcgaacccg gtacatcggt
     7261 gatcgacggg taggtcttga ccccgaggat catgtcgacc ttgggattga tcggatagac
     7321 cttgcccgcg tagccgtcct tgatcaggcc gaccatcgcc ttgtagccgc gtttggtggg
     7381 gtcggccgag gcgccgacga tggcgatcga atcgggtgcg agaaagtcgt gcagcgggct
     7441 gcgggtgttc gttacggtca tggtcagcgt cctttgaaaa cggggctgcg tttctcggcg
     7501 aaggcatcaa tgccttcctg ccagtcggcg gtcgtgccga caaacatcat gccctcgagt
     7561 tcggcggtca gcaccgcgtc gagcgtgcgc tcgccggaga ggttgagctg ttccttggcg
     7621 agctgcatcg agaacggcgc tttgccggca accttttttg cgaaggcgcg tacgtcgtca
     7681 cgaaacgagg cgtcggcaaa gcagcggttg gcgaggccga tgcggactgc ttctttgccg
     7741 tcgattcgct cgccgagaaa gaccagctcg cgggccttgg cgaggccaac gagtcggggg
     7801 agcagggcgg tcacgccgcc gccgaggaaa ttgccgaggc tgatctcggg caggccgatc
     7861 tgcgcgcctt cggccatcag caggaagtcc gaagcgatcg ccagctcggc gccggcgccg
     7921 agcgcgtagc cgttgacggc agcgacgacc ggcttggaca gcagcatcag gcgcttacag
     7981 acaatctgct cgccgcgcag gtactgtcgg cgatcaaagg ccgtgcgtcc ggccttgtgt
     8041 tccttgaggt cggcgccgac gcagaaggcg cggccttcgc cggagaggac cacgacgcgc
     8101 acatcgggat cggactcggc gacgccgaga gcgtcattca gttcgtcgta gagctgctgg
     8161 gtgacggcgt tgagccgttg cggccggttt agccggattt cggcgatgcc atcggtactg
     8221 gaataaacga ttgtctgcgc gtccatcgaa tctctcccgg aattaagtgc ggcgctggcg
     8281 ggttcggcat tggcacgacg agttggcgtc gtcgcgtcag gtgacggcca gacgcgaggc
     8341 gacgccgaag cgagaccctg gcatatcgaa gcgatggaaa agtaggcgcg tgcaggaggc
     8401 gttcgccgcg cgcaaaccgc aaatgccgag gccggtgccg gacggacttg cgatgacgtt
     8461 gttcttgatt ggtccggctg attcagggta gcacaagcgg cgggagagcc gccgcctgtg
     8521 ccgctgtttc gtacaaacaa agtagcgaaa tcacagctct ggtgggcgtt tacgccgaat
     8581 tacatcgttc tgaacaggat ttttcctcat gaagagtcct tgtgctgagg cggcggtaac
     8641 acacggaaaa gaagaagtat tctttggtct attgttgcga tgatcgatct cgggcggtgc
     8701 gattgttgcg tgccttacac gtagcgtgaa ttttcagctt cattagtcga gtgcaaggct
     8761 gcggaaccga ctacaagctt agttacagcg ccagttgttg gagttccggt gacatgggga
     8821 tgcgcgcttg tcgccgggcg agcgctgcat atctttatcg aatcgctgac tgaagcggaa
     8881 cggtaagaac agcgactggc tcgctaccac gctgctatgg gcgatctgtc aggcggactg
     8941 cggaagcttg tccagatgcc actaccaatc gccgcaagta gtgccttggc attggcatct
     9001 aaggccggcg tcgagacgct cgggggataa tgtcaaacgg gcagggaagg gcaggtcgtc
     9061 ggcctgagcg gcccttgggt gtccggccag actcaggcgg caatgcgccg gtcacctgcc
     9121 gttcagatgt gattcgcgtc ggacggcagc ccctcgttcg gggaactcgc actatcggta
     9181 gagtcgagat gtggcgcggt agcagtaggt tcagcttgtc tgttgccgcc cctttcgtct
     9241 ggcggtgccc aagtagcctt gccgtaaccc cgtttccaca ttccggtcat cgaaccggac
     9301 atgcagattt cccgcatccg gctctcagac aagacatcac gccttcgccc acgccttgtt
     9361 gcgcccgccc aggctggcaa gaccgaagta cccgtaaagg tgcgggagtg gataagtcga
     9421 tcctgcgctc cgcgaatggc tgaacccccc cgatttcaga cttgctcctg accctgtcgt
     9481 tgccgccggc gggcggcgcc gacccgacaa cgggacagac gtcatctcgg gggtggctgc
     9541 ggtacgctcc atggaaacga tcaatggctc agcgaggtcg gcggaatgcg tgcatatccg
     9601 ggaatgggtg cgccccctat ttgacatcat tgatgacgca taatccgggc tgactaccca
     9661 taagggctac ccatcgggcc ccatcccggt agttgacgct gggcggtgcc ttcttgccca
     9721 atgccggttc cgtaatgagc gccgcatcac agaccaagaa caccctcttg atcgagatca
     9781 gaacctgacc accgatccga ccgaatgacg gccgcaaggc tgtggaaaga cgtttcaagg
     9841 agaccgcacc ccatgctcac ccagaaccca tttgctctat tgaacagcgc cttgcccgag
     9901 agc
//
LOCUS       DATDAA010000227         4612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_331153,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000227 DATDAA010000000
VERSION     DATDAA010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331153"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VIS73_07225"
     CDS             <1..334
                     /locus_tag="VIS73_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY5762983.1"
                     /translation="GGLSSLIDKLPAQVAQAAGQMPVGMEDKMVRRIEGIINSMTPLE
                     RAKPELLKASRKRRIATGAGVPVQEVNRLLNQFEQTQKMMKQFSKGGLGKLMRGMKGM
                     KGLMPGMR"
     gene            complement(362..976)
                     /locus_tag="VIS73_07230"
     CDS             complement(362..976)
                     /locus_tag="VIS73_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY5762984.1"
                     /translation="MHGLSLRLVAALLAVAAAGAVQAGAQRYEPLSASVQAALSKSLA
                     DRAPDGTSFADRGEAAVWLAQMSRRLEKRIPDQAERLDFLRAVHYEATRAGLDPELVL
                     GLIQVESAFRKYAVSKAGARGFMQVMPFWVDLIGRKSDNLFHLRTNLRYGCTILRHYL
                     DIENGNLFRALGRYNGSLGRAKYPDAVRAAWHRHWKLEVRHVGG"
     gene            complement(964..2682)
                     /locus_tag="VIS73_07235"
     CDS             complement(964..2682)
                     /locus_tag="VIS73_07235"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY5762985.1"
                     /translation="MRSSQFFFTTLKEAPSDAELISHQLMLRAGYIKRIAAGVYTWMP
                     VGLRVMRKVENIVREEMNRAGAVELLMPAVQPGELWQESGRWEQYGPELLRLKDRHQR
                     DFVIGPTHEEVITDTVRRDVKSYRQLPVHLYQIQMKFRDEIRPRFGVMRGREFLMKDG
                     YSFHAGFADLEREYRNMFDTYSRIFTRLGLAFRAVAADTGSIGGTGSHEFHVLADSGE
                     DALAYCPDSDYAANVELAEGLPAPDASRSAATQAMQKVATPETTRCEDVAQMLGQKLQ
                     QTVKAIAVMHNAEFVLMLLRGDHALNEIKASKLPGLNPFRFATDAEIREHMGCAAGYL
                     GPVSIKSSVRLIVDRSVALMSDFTCGANDEGYHLTGVNWGRDLPEPALVADIRNVIDG
                     DPSPDGKGSLSLCRGIEVGHIFQLRTKYSQAMNCNFIDEAGQSQAMEMGCYGIGVTRI
                     VGAAIEQGHDERGIVLPASIAPFSVAIVPMAYHKSEAVRAAADALYAELREAGLDVLL
                     EDRNERPGVLFADMELIGVPHRIVVGERGLANNEIEYKGRRDEAARMVARNDAFALLR
                     AEGCTA"
     gene            complement(2815..3711)
                     /locus_tag="VIS73_07240"
     CDS             complement(2815..3711)
                     /locus_tag="VIS73_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762986.1"
                     /translation="MKPRFAAIAAILLAQAIPVFASPPLVERLDRHERLLADGAGPPD
                     HDGSSWRLDPLPAIPPPAGCVPAAPPETLLAEPRVDLFRCQAGFANTLVGDGDGGSRW
                     TRELRHRSGPHTIELYVGSANAHAITLDTMEVIDPASGRTLKFAPMKLIDTPPRAMPI
                     VRVSGALACPPDGGDCFGAIGEGDPAGLLRISRDGSVSVLEKPQTRWFAPIVITDIQI
                     AGDRVLLAEEWLYRGSKWVRFAVVDAVTGKRLFEERHGEDHVAGSPRIVSGPAGEIAF
                     HYRDATIGRMVMVRYRLARRAD"
     gene            complement(3717..>4612)
                     /locus_tag="VIS73_07245"
     CDS             complement(3717..>4612)
                     /locus_tag="VIS73_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY5762987.1"
                     /translation="HDTIEGLEALDKVINVDQSPIGRTPRSNPATYTGLLTPIRDLFS
                     QVPEARARGYGPGRFSFNVKGGRCEACQGDGMIKVEMHFLPDVYVPCEVCHGRRYNRE
                     TLEIHYKGKTIHDVLQMTVEMAREFFDAVPVVARKLQTLVDVGLSYIQLGQSATTLSG
                     GEAQRVKLALELSKRDTGRTLYILDEPTTGLHFHDIEMLLMVLQRLADHGNTVVVIEH
                     NLDVIKTADWIIDLGPEGGGGGGRIVAAGSPDTVAAVADSHTGRFLAEMLAGRHRIPA
                     AATEPPRSARAAAAKKRGKAA"
BASE COUNT          721 a         1454 c         1620 g          817 t
ORIGIN      
        1 gggcgggctt tcctcgctga tcgataagct gccggcgcag gtggcgcagg ctgccgggca
       61 gatgccggtg ggcatggagg acaagatggt tcgccgcatc gagggcatta tcaattcgat
      121 gacgcctctc gaacgggcga agccagagct gctcaaggcg tcgcgcaagc gacggatcgc
      181 tactggcgcc ggcgtgccgg tgcaggaggt caaccgcctg ctcaatcagt tcgagcagac
      241 acagaagatg atgaagcagt tttccaaggg cggactgggc aagctcatgc gcgggatgaa
      301 gggcatgaag ggtctgatgc ccggcatgcg ctgagcgccg ccgcgcgacg ctgccacgtc
      361 gctagccgcc gacgtggcgg acttccagct tccagtgccg atgccaggcg gcgcgcacgg
      421 cgtccgggta cttggcgcgg ccgaggctgc cgttgtagcg gccgagtgcg cggaagaggt
      481 tgccgttctc gatgtcgagg tagtggcgca ggatcgtgca gccgtagcgc agattggtcc
      541 gcaaatgaaa caggttgtcc gacttgcggc cgatcaggtc gacccagaag ggcatcacct
      601 gcatgaagcc acgggcgccg gctttggata cggcgtactt gcggaaggcg ctttccacct
      661 gaatcaggcc cagcaccagt tcgggatcga gcccggcacg cgtcgcctcg taatggactg
      721 cgcgcaggaa gtcgaggcgc tcggcctgat cgggaattcg cttctccagc cggcgcgaca
      781 tctgcgccag ccagacggct gcctcgccgc gatcggcaaa cgaggtgccg tcgggcgccc
      841 ggtcggcaag gctcttcgac agcgccgcct gcacgctcgc cgacagcggc tcatagcgtt
      901 gtgcaccggc ctgcacggcg ccggcggccg ccaccgccag cagtgcggct acgaggcgaa
      961 ggctcaggcc gtgcatcctt cggccctgag cagcgcgaat gcgtcgttgc gcgcgaccat
     1021 gcgcgctgct tcgtcgcggc gacccttgta ttcgatttcg ttgttggcca gtccgcgttc
     1081 gccgacgacg attcgatgag gcacgccgat cagctccatg tcggcgaaca gcactcccgg
     1141 tcgctcgttg cggtcttcga gcaggacgtc gagtccggcc tcgcgcagtt cggcgtagag
     1201 cgcgtcggca gcggcgcgga ccgcctcgct cttgtgataa gccatcggca cgatcgccac
     1261 ggaaaacggc gcgatgctcg ccggcagcac gatgccgcgc tcgtcgtggc cctgttcgat
     1321 cgcagcgccg acgatgcggg tcacgccgat gccgtagcag cccatctcca ttgcctgcga
     1381 ctgtcccgct tcgtctatga agttgcagtt cattgcctgc gagtacttgg tacgcagctg
     1441 gaagatgtga ccgacctcga tgccgcggca gagcgacagc gagcccttgc cgtcggggga
     1501 cgggtcgccg tcgatgacgt tgcggatgtc ggcaaccagc gccggctcgg gcaggtcgcg
     1561 tccccagttc acgccggtaa ggtggtagcc ctcgtcgttg gcgccgcagg tgaagtcgct
     1621 catcagcgcg acgctgcgat cgacgatcag ccgtactgac gacttgatgc ttaccggtcc
     1681 cagatacccg gcggcgcagc ccatgtgttc gcgaatctcg gcatcggtgg cgaagcggaa
     1741 ggggttcagg ccgggcagct tcgacgcctt gatctcgttc agcgcatggt cgccgcgcaa
     1801 gagcatcagc acgaactcgg cgttgtgcat tacggcgata gccttcacag tctgctggag
     1861 cttctgcccg agcatctgtg cgacgtcttc gcagcgcgtg gtctccggcg ttgccacctt
     1921 ctgcatcgcc tgggtcgctg cgctacggct ggcgtcgggt gctggcaagc cttcggcgag
     1981 ttcgacgttg gcggcgtagt cggagtcggg gcagtaggcc agcgcgtcct cgccagagtc
     2041 ggcgagtacg tggaattcat gcgagcctgt gccgccgatc gagccggtgt cggcggccac
     2101 cgcgcggaaa gcgagaccca gacgggtgaa gatgcgcgag taggtgtcga acatgttgcg
     2161 gtactcgcgc tcgaggtcgg cgaagcccgc atgaaaggag tagccgtcct tcatcaggaa
     2221 ctcgcggccg cgcatgacgc cgaagcgggg ccggatctcg tcgcggaact tcatctggat
     2281 ctggtacagg tgaaccggca gctggcgata gctcttgacg tctcggcgga ccgtgtcggt
     2341 aatcacttcc tcgtgcgtcg gcccgatcac gaagtcgcgc tggtggcgat ccttcaggcg
     2401 cagcagttcc ggcccgtact gctcccagcg gcccgattcc tgccacagct cgccgggctg
     2461 cacggccggc atcagcaact cgaccgcgcc ggcgcgattc atttcctcgc ggacgatgtt
     2521 ctccaccttg cgcatcacgc gcaggccgac gggcatccag gtatagacgc cggcggcgat
     2581 gcgtttgatg tagccggcgc gcagcattag ctggtggctg atcagctcgg catcggacgg
     2641 cgcttccttg agggtggtga agaaaaactg gcttgatcgc atgcgggggg attccactgc
     2701 tggtttcgag gatggccgat tctagcggaa aggccgtcag atgggcgctc tacgtgcggt
     2761 gcggcggttg tgtctggagg cttgaccggc gtagatcgcc gcacgacgtg cgagtcagtc
     2821 ggcccggcgt gcgagccggt agcggaccat gaccatgcgt cctatcgtcg cgtcgcggta
     2881 gtggaaggcg atttcgccgg ccgggccgga cacgatgcgt ggactgccgg caacgtgatc
     2941 ctcgccgtgg cgttcttcga acagtctctt gccggtgacc gcgtcgacga ccgcgaagcg
     3001 gacccacttg ctgccacgat agagccattc ctcggcgagc agcacgcgat cgccggcgat
     3061 ctggatgtcg gtgatgacga tcggcgcaaa ccacctggtc tgcggttttt cgagcacgct
     3121 gacgctgccg tcgcggctga tgcgcaacag cccggccggg tcgccctcgc cgatcgcgcc
     3181 gaagcagtcg ccaccgtcgg gcgggcaggc cagcgcgccg ctgacgcgga ctatcggcat
     3241 cgcccgtggc ggggtgtcga tcagtttcat cggcgcgaac ttcagcgtcc ggccactcgc
     3301 cggatcgatg acttccatcg tatcgagggt gatcgcatgc gcattggcgc tgccgacgta
     3361 gagctctatc gtgtgcggcc cggagcggtg gcgcaactcg cgcgtccagc gcgagccgcc
     3421 gtcgccgtcg ccgaccagcg tattcgcaaa cccggcctgg cagcgaaaca gatcgacgcg
     3481 cggctcggcg agcagggttt cgggcggggc ggcgggaacg catccggccg gtggtggaat
     3541 cgccggcagc gggtcgaggc gccagctgct gccgtcgtga tcgggcgggc cggcgccgtc
     3601 tgccagcaag cgctcgtggc gatctaggcg ctctacgagt ggcggcgagg cgaataccgg
     3661 gattgcctgt gccagcagga tcgcggcaat cgcggcgaat cgcggcttca cgggattcag
     3721 gcagccttgc cgcgtttttt cgccgccgcg gcccgggccg atctcggtgg ctcggtggcc
     3781 gccgccggta tgcggtgcct gccggccagc atctcggcta gaaagcgccc agtatggcta
     3841 tcggcgacgg cggccacggt gtcgggcgag ccggcggcga cgatgcgtcc gccgccaccg
     3901 ccgccttcgg ggccgaggtc gatgatccag tcggcggtct tgatcacgtc gaggttgtgc
     3961 tcgatgacga cgacggtgtt gccgtggtcg gcgaggcgct gcagcaccat caggagcatc
     4021 tcgatgtcgt ggaaatgcag gccggtggtc ggttcgtcga ggatgtagag cgtgcggccg
     4081 gtgtcgcgct tcgacagttc gagcgccagc ttgacccgct gcgcctcacc gcccgagagt
     4141 gtggtcgcgc tctgcccgag ctggatgtac gagaggccga cgtcgaccag cgtctgcagc
     4201 ttgcgggcga cgaccggtac cgcgtcgaag aattcacggg ccatctccac ggtcatctgc
     4261 agcacgtcgt ggatggtctt gcccttgtag tggatctcca acgtctcgcg gttgtagcgc
     4321 cggccgtggc agacctcgca ggggacgtag acgtcgggca ggaagtgcat ctcgaccttg
     4381 atcatgccgt cgccctggca ggcttcgcag cggccaccct tgacgttgaa ggaaaagcgc
     4441 cccgggccgt agccgcgcgc ccgcgcttcc ggcacctgcg agaaaaggtc tcggatcggc
     4501 gtcaagaggc cggtgtaggt agccggattg gagcgtggcg tgcggccgat cgggctctgg
     4561 tcgacgttga tgaccttgtc cagcgcctcg agtccctcga tcgtgtcgtg cg
//
LOCUS       DATDAA010000228        18409 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_121905,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000228 DATDAA010000000
VERSION     DATDAA010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 18409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18409
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121905"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..1721
                     /locus_tag="VIS73_07250"
     CDS             351..1721
                     /locus_tag="VIS73_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5762988.1"
                     /translation="MKKDEMPEKIGRYEIIRKLGKGATASVYLARDSDSDLQVAIKLI
                     EFDSRGTALRRRLEKLFKTEIDVARKLDHPNIVHVHDAVINEEHAYIVMEYVEGSPLD
                     AYASADRLLPMDRVIGIAFKCALALDHAAQRGVVHRDIKPANILVDKDDNPKLTDFGL
                     ALFLQKDMEQDSTFVMGVGSPAYMSPEQVKGYPLSHKTDLYSLGVVLFQLLTGRLPYR
                     ASNQAALIYKIINAEIPDITKLNPQLPAEVGKIMHRVLEKDTYTRYRSGADLAKDLTA
                     VRYGIVGEKDDGGERDHKRFGILRHITFFKAFDDLELWEALRLCVWRDLEPKVTLMQE
                     GEADKRFHIIVAGLVEVSTSGKALCRLGAGEVVGEMAYLHPDEPLRNATVVTLEPTTV
                     VEFNAAALNLSSEELQEAFQKVLITTVLNRLRAANAALAKHGDIAIIGSPMHRGDLEL
                     APPIDL"
     gene            complement(1726..2142)
                     /gene="msrB"
                     /locus_tag="VIS73_07255"
     CDS             complement(1726..2142)
                     /gene="msrB"
                     /locus_tag="VIS73_07255"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY5762989.1"
                     /translation="MPELKKPAAEWRQRLSSERFAVLFQEATERPFSSPLNDEKRDGT
                     FVCAACANPLFDSAQKFDSGTGWPSFWQPLPDALGTKTDFKMILPRSEYHCARCGGHQ
                     GHVFKDGPKPTGLRYCNNGLAIEFVPRGEALPPLKD"
     gene            complement(2227..2817)
                     /locus_tag="VIS73_07260"
     CDS             complement(2227..2817)
                     /locus_tag="VIS73_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5762990.1"
                     /translation="MSRYQAIDLSALRIGAVCRQPSTYHLVQLDSPAIDVMTDFQTTT
                     PAMIRPDATVSQAASIMVSRGVRFLFVVNDDDAIIGVVTARDLTGDRATRAVREKGGN
                     VGELKVDLIMTAQSDMQAMPIEAVMPAKVGDILETLRRVGRQHAIVTEKHAGSGVDLV
                     RGIFSATHIGRRLGVPVASFEVAKTFEEIHAALADT"
     gene            complement(2893..3792)
                     /locus_tag="VIS73_07265"
     CDS             complement(2893..3792)
                     /locus_tag="VIS73_07265"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HEY5762991.1"
                     /translation="MARKEIAFYPGCSSQLKASASNYLVSARSMCDKLDIKMTEIPDW
                     NCCGASIGYAEGGELPRLAITARNFALAEQHLPGQDMVSTCAACWLATREAKERLENS
                     NVLMKETNEALAAAGLNYKGEVKVRHMVEVLIEDLGYEELAKPVIKPLQGIKFAGYVG
                     CQTNRPFGIDGESFENPKYLDKLVESCGGEAVEKYDQKVTCCGGGLAFSEPDKSQKQI
                     RDIVESAYDNGAEMIVTPCPLCQANVEVYQSEINKKQGTKFNMPVLYYSQLLTIAYGG
                     SAKDAGLDGQVIRAPKLEEIAGK"
     gene            complement(3819..4442)
                     /locus_tag="VIS73_07270"
     CDS             complement(3819..4442)
                     /locus_tag="VIS73_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY5762992.1"
                     /translation="MTQANAQAVAKYRNDFLREVEERVEEGHMVKMCMQCGVCAGSCP
                     FGPHWEHSPQKIFMMIRAGKRDEVLQSDAMWMCTSCYNCMVRCPRQLPITHIMHGLAN
                     YSHRLGIAPKGQPTRDFAKLFWDNLTKRGRVNELTLTLGHYFKDGLVSGVKTAMSMQK
                     IGLGMLKAKRLNPMEIFGGHKVADLSGFHAIVAKAREIEDRKKGLKA"
     gene            complement(4445..6649)
                     /locus_tag="VIS73_07275"
     CDS             complement(4445..6649)
                     /locus_tag="VIS73_07275"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5762993.1"
                     /translation="MSEMKTAAYLCTGCGIGDKLDVKTMEKIATKEGKMALVKNHAFL
                     CGEEGVAMIKNDIANEGVTHVMIGACSRRAKTEAFHFPTAAMARANLREGVLWVVAEG
                     DEHDEVRQEMADDYVRMGCAELKKMKIPAGNPNTATNKRVLVVGGGFTGMTAALEVAT
                     TGYEVLLVEKTGALGGWAGKAWRTVPDREPHADPQPTGIPELIEAVGKHPKIKVLLNS
                     TLAETGGAPGRFAVSIATESGGTTSEEVGAIIQANGFTPYAIDKLPELGGGKLANVVD
                     HAGLEALATAANGGAIKRADGKEVKSVAFLQCAGQRDETGMHLSYCSGHCCATSVLQA
                     RYFKDHNPDVDTVVIYQDLRMPGMGEDFYRAAQEKGVIFTKGKASAVEAAGDAAKVSF
                     RDLILDEDTSLEADLVVLATGQVPNSGQNIDLAEDDPVRTEKVSILNLNYRQGKDLPI
                     LRHGFNDSHFICFPYETRRTGIYASGPVRRPMDMAQAREDATGAALKAIQAVENASVG
                     RAAHPRSGDLSYPIVRLEGCTQCKRCTVECPFGAIDEDEKRFPVFNESRCRRCGTCMG
                     ACPVRVISFENYNVDTVGAQIKCLDMPDEFSEKPRILILACENDAYPALDMAGLQRHV
                     YSAFIRAIPVRCLGSVNLVWVTDALNSGWDGIMMMGCKKGDDYQCHFVKGSEMAHYRM
                     SKVDDTLTQLGLEKERVITHEIAITDNARCAALINEYAEQIKELGLSPMKGF"
     gene            complement(6646..7944)
                     /locus_tag="VIS73_07280"
     CDS             complement(6646..7944)
                     /locus_tag="VIS73_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5762994.1"
                     /translation="MTGTIATNSTLLVVGGGISGMTAALEAAECGKDVILVEKLPTLG
                     GRTSRLYRYFPKMCHPTCGLEINLRRLKAQQANGRIRVMTMTEVTGISGSRGNYTVSL
                     NVAPRFVNEKCTACGDCAKAVSTEIDDTYEYKLKKTKAAYLPHAMAYPQRYVLDPSIV
                     GGPCGDAAKAACKVDAIDLGMKDEDVTLNVGAVVWATGWRPYDAAKIQPYGYDRFANV
                     ITSLEFERLADPHGPTGGKLLRPSDGKEAKNIAFIQCAGSRDENHLRHCSRICCMASL
                     KQTHYVREAYPDGSGKSTVYYIDIRAIDRFEDFYQAVQKDETVSFIKSKVARIAEDKE
                     QNPVLHGVDTEGYHRYATPHDLVVLAVGMEPETQGIQLPDDIVLDSSGFIEGSKDQGM
                     FGAGAASSPLDVNRSTQSATAAALRAIQVIHRAAATETQA"
     gene            complement(8133..8651)
                     /locus_tag="VIS73_07285"
     CDS             complement(8133..8651)
                     /locus_tag="VIS73_07285"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HEY5762995.1"
                     /translation="MKICIVSDSHDRGDSLARAIAAGKAEGATAVIHCGDIIGTNTLK
                     ASLAIDIPQHVIHGNNLGDPASLGRLTVRMNGRLAYYGQDATLELGGRRLFVVHYPEY
                     GEAMALTGRYDVVCVGHSHEPEVRTVRNVNGTDTWLVNPGTVAGLGAPASTWILGDLA
                     TCEFTVRAVPPA"
     gene            complement(8658..9062)
                     /locus_tag="VIS73_07290"
     CDS             complement(8658..9062)
                     /locus_tag="VIS73_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762996.1"
                     /translation="MNAPEFSEHERKLVQQTLLERYGTPVPIQSVEVELQLDPPSPVL
                     TDCHAFYWQRDGAEFVVCKAARDDYRAQFFYGPEEIFGTGIDSYDNLGDCIVSLLQVQ
                     AAHAAERPATGDAPQRVQPKVTGDDYDGPLVI"
     gene            9222..9635
                     /locus_tag="VIS73_07295"
     CDS             9222..9635
                     /locus_tag="VIS73_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEY5762997.1"
                     /translation="MDQNHDPKRHSAAGAAPAGQALRGIPVRAAPAQVSSCAGEEAFA
                     LQVLGSDMAPEFVDGDIIIVEPDGLVSDGSFVVARHDGEWLFRQIVRRDAGWVLVALA
                     DGVPEHALSGLDAVRGVIIEKTRPGRRRERKRYVD"
     gene            9610..10719
                     /locus_tag="VIS73_07300"
     CDS             9610..10719
                     /locus_tag="VIS73_07300"
                     /inference="COORDINATES: protein motif:HMM:NF044557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5762998.1"
                     /translation="MSASAMSTEMLDVLPRDVLPRLIETAQFNADLADAHFAQAATLC
                     IYLLDMREYYRWLNALPLDAKLPREHLGPWIAEQEALWDTLRADGQEQREYRPLDPAV
                     SGDPFDTRSVHELLVSGGLVYGAGIGRFGRPLFFLAECLRRETREGIDIAVCGRELAR
                     GSSAPPAMSRDAQVIVRRDALERWLWTRYEEWRHHPRENGFVAAWLLHSGGVAPGDHP
                     DPKAIVGRIADAEIETLILHEIGERRIDALVGDVWHDMIDDLGNRKLEVFARSARDLL
                     ADCGHTLPTLLERGDMASLHCWFGLLDGMRLKFSPTLVAAYRATGGKPDAALGRVLAE
                     AAEHWQGVCTQLVDHWQGGGSAAVQALFDSGAVGH"
     gene            10790..11182
                     /locus_tag="VIS73_07305"
     CDS             10790..11182
                     /locus_tag="VIS73_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY5762999.1"
                     /translation="MKHLLPKEAFEFLKQNPDALFIDVRSEMEFLFVGHPKDALHVAW
                     NDGPDWDINPHFVGEVKKLAGAMSARPMVLICRSGKRSAEAGEALKAAGFHNVYNVTH
                     GFEGDLDEKHHRGSVNGWRCDGLPWEQC"
     gene            11240..11719
                     /locus_tag="VIS73_07310"
     CDS             11240..11719
                     /locus_tag="VIS73_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5763000.1"
                     /translation="MIEIRPAAFPRDLEVVRALFREYAAGLGIDLCFQGFEEELAALP
                     GKYAPPGGRLLLAEEAGETLGCVALRPVDASTGEMKRLYVRPAARGRQLGRQLAERIC
                     AEACKAGYSRICLDTLASMNAALALYASLGFRPVDAYVFNPLPDVVYLGLALTPAAN"
     gene            complement(11712..12119)
                     /locus_tag="VIS73_07315"
     CDS             complement(11712..12119)
                     /locus_tag="VIS73_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY5763001.1"
                     /translation="MEHFEAEWAGEQGRGFVVVADEVRKLAERSTAAAREIDGVTAVL
                     SQRSAQVESAIAAGLRSLEASHDCANCGIDEISLSVIEQRTASQDIARNVEKIANMID
                     ENHAAMSDAPTAANSLKSTAGNPRSLVNRFRVN"
     gene            complement(12130..12741)
                     /locus_tag="VIS73_07320"
     CDS             complement(12130..12741)
                     /locus_tag="VIS73_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763002.1"
                     /translation="MAILKESNQLQRRFLGSLLLISILTGTSGCAGIFIDRTSSAVTE
                     RPIPLRAPLDAKSDFDRWACQPTEDSRPATKDDFLIAWGAPLRKDTHGNTEVWHYEEK
                     GRWCGVWIAFGLPIPLLLPACETHDEVTFENDMSVRARSKRFVRTIFGITLIPAPPGA
                     MPVFTRAGNADEIGPAINIFPGQSDRRCRWPAAESLQTVELER"
     gene            complement(12932..14317)
                     /gene="cysG"
                     /locus_tag="VIS73_07325"
     CDS             complement(12932..14317)
                     /gene="cysG"
                     /locus_tag="VIS73_07325"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005176113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HEY5763003.1"
                     /translation="MDRLPIFLDLKGRACAVVGGGEVAARKVSLLLSAGARVTVSAPA
                     LCPSLAAEANAGRIAHHAVAYQPAALDDAVLVIAATNDRTVNAAVSRDAQARKLPVNV
                     VDDPELCSFIMPAIVDRSPMIVAVSTGGASPVLARLMRARLESLIPAAFGRLVELADR
                     FRDRVKAALPESRRRQFWESTLQGPVAELAVSGREREAEEQLDAALIAATRGDSTSAS
                     GEVYLVGAGPGNPDLLTFAALRLMQQADVVLYDNLVAPAIVDLARRDAERIYVGKRGA
                     DHSLPQPDINALLVEHAQAGRRVLRLKGGDPYVFGRGGEEIATLAAHGIRFQVIPGIT
                     AATGAASFAGIPLTHRDHADSCAFVTGHLKDGRLDLDWPALVRPRQTVVVYMGLPGVA
                     ELCRQLIANGRAPTTPVALIARATLPDQIVVAATLETLADRVAAAQPPSPTLIIIGDT
                     VALRGQLSWRN"
     gene            complement(14355..14978)
                     /locus_tag="VIS73_07330"
     CDS             complement(14355..14978)
                     /locus_tag="VIS73_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY5763004.1"
                     /translation="MSDALNYLVKKRPEAMGAYFSFLKDAGTRLDPKTRNLISVITKV
                     HAQTENGLKQYLKRALRDGASGEEVIDALLMAFPALGLAKIVWATDIILQMNLPEFAL
                     DGATAGSWHDLGAKRDIKVGETVRLDCDGRSVFIYRGKKKFRVYDSLCPHQTTNIPHL
                     ALKGTTLTCPKHKWAFDITTGACTKVGDTPLKEWESKTEKGRLLAYW"
     gene            15172..17007
                     /locus_tag="VIS73_07335"
     CDS             15172..17007
                     /locus_tag="VIS73_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY5763005.1"
                     /translation="MSARSRLINRVFPFMVWRHRVTRTTLRDDLIAALIGAVIVMPQG
                     VAFATLAGLPPQYGLYAAMVPAIVGALFGSSWQLVSGPTNAISLVVFATLSPLAIPGS
                     EKYVALAITLACMVGVMQLAMGIARMGAVVNFISHTVVVAFTSGAAILIMASQVRNFF
                     GIPIEAGSSFLHTVAELATHLGSVNLWVLAVALTTILAGILSRRMFPRFPYLLSAMFA
                     GALLGTALNAGFGIDETGIRTLGALPAALPPLSRPTFDLDTWGRLVGIAVAVTALGLT
                     EAVSIARSIATKTGQRIDGDTEFIGQGMSNFIGSFFSAYPSSGSFNRSGANYEAGART
                     PLAAAFSGVFLGLAVFVIAPLAAYLPYAAMAGILVLVAWGLLDFHEIITIARTSRAEA
                     AVLAVTFFGTLVMHLEMAIVAGVMLSLLLYLNRTARPTIRSLVPEPDTSLGRFVERKP
                     GMAECPQLKILRIEGELYFGAVNHVAEYFHFLADHRPGQKHLLLMSRSMNFIDVAGAE
                     LLAAEARRRRDAGGSLSFHGLRVAAGDMLSQPAFKNEFTGRFTGKTEAIAGCFALFDR
                     DICRSCTARIFRECNSVPYEPVAAADVLPPADADQNGKKIGSSSE"
     gene            complement(16971..>18409)
                     /locus_tag="VIS73_07340"
     CDS             complement(16971..>18409)
                     /locus_tag="VIS73_07340"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY5763006.1"
                     /translation="MLMLPILTGAALRSKTPPGKMLMPANYAVLIGGMGTTIGTSTNL
                     LVVAIAADLGVRQFGMFDFFHLTAIAAVGGILYLWLIAPHLLPTRDTPLGDAAPRLFH
                     TKLTIEKDGPADGATFAELRKLVGQEGRIVRIRRESGVELARLPSLKMHAGDRLEIVG
                     PGKLIAEVGDTLGAVAAELPEDEEEEVENKGEPQQVAEAVVTENSPLNGRTVRSMRIA
                     DDYGVVIVALHRPQMDTISAQQDIADVTLRVGDVLLVQGTQDDIQELRDRTGLLVLSG
                     RLTLKATERAPAALAIFALTIVLAAFKVLPIAVAAMMGVALMILSRCVGWRDVGNALS
                     GKVILLVVSSLALGSALVQTGTTAALAQSALSIADGASPLVLVIVLMSLMLVLTNFVS
                     NNAAAAIGTPIAVDIARTLGVAPEPFVLAILFGANFCYLTPMAYQTNLLIMTPGGYRF
                     VDYLRVGAPLAVLMLTSYSLLLPIFFPF"
BASE COUNT         2943 a         5763 c         6219 g         3484 t
ORIGIN      
        1 gctcctcgat cgacggctcg gcggcaggcg ttgcgtcgcc ggcgggctgc tcggccggag
       61 cctgcgcttc ggtttcggcc tcgagggcca gggcctgggg ttcctgctgt tgctccggtt
      121 gttcgttcga catcgcgttc cactggtcct gaaattaggt catcggaccc tgatgggggc
      181 agatgacgca tcttcaagtg tcgatgggtg gtttttttgc tgaaaggcac cggtgatggc
      241 cgccgattga cgccggattg tggtgccgat gcgcgttcgt cggtgcggga atggccatgg
      301 gagacttgcc gatggcccaa agccgggccg gctgcggtag attaggcacc atgaagaaag
      361 acgagatgcc cgagaagatc gggcgctacg aaataattcg caaactgggc aagggcgcca
      421 ccgcttcggt ctacctcgcg cgcgactccg attcggacct gcaggtggcg atcaagctga
      481 tcgagttcga cagccgcggc acggcgctgc gccgccgcct cgaaaagctt ttcaagaccg
      541 agatcgacgt ggcgcgaaag ctcgaccacc cgaacatcgt ccacgtccac gatgcggtga
      601 tcaacgagga gcacgcctac atcgtcatgg agtacgtcga ggggtcgccg ctcgacgcct
      661 acgccagcgc cgaccggttg ctgccgatgg accgggtcat cggcatcgct tttaagtgcg
      721 cgctggcgct agaccacgca gcccagcgtg gcgtcgtcca tcgcgacatc aagccggcca
      781 acattctggt agacaaggac gataacccca agcttaccga tttcgggctc gccttgttcc
      841 tgcagaagga catggagcag gactcgacct tcgtcatggg tgtcggctcg ccggcctaca
      901 tgtcgccgga gcaggtcaag ggctatccgc tgagccacaa gaccgacctc tactcgctgg
      961 gggtcgtgct gtttcagctg ctgaccgggc ggcttccgta tcgtgcgtcg aatcaggcgg
     1021 cgctgatcta caagatcatc aacgccgaaa ttcccgacat caccaagctc aatccgcagc
     1081 tgccggccga ggtcggcaag atcatgcatc gggtgctgga gaaggacacc tatacgcgct
     1141 atcgctccgg ggccgattta gccaaggacc tcacggcagt tcgctacggc atcgtcggcg
     1201 aaaaggatga tggcggcgag cgcgaccaca agcgcttcgg catcctccgc cacatcacct
     1261 ttttcaaggc tttcgacgac ctagaactgt gggaggcgct gcgcctgtgc gtgtggcgcg
     1321 atctcgagcc gaaggtgacg ctgatgcagg aaggcgaagc cgacaagcgc tttcacatca
     1381 tcgtcgccgg gctggtcgag gtgtcgacat cgggcaaggc gctgtgccgt ctcggcgccg
     1441 gtgaagtcgt tggcgagatg gcctacctgc acccggacga acccttgcgc aacgcgacag
     1501 tcgtcacgct ggagccgacc accgtcgtcg agttcaacgc cgcagcgttg aacctctcct
     1561 cagaagagct tcaggaggcc ttccagaagg tgttgatcac caccgtgcta aaccgtctgc
     1621 gcgccgccaa tgcggctctg gccaagcacg gcgacatcgc catcatcggt tcgccgatgc
     1681 accgcggcga cctcgaactg gcgccgccga tcgacctctg aaggctcagt ccttcagcgg
     1741 cggcaacgct tcgccacgcg gaacgaactc gatcgctaag ccgttgttgc agtagcgcaa
     1801 cccggtcggc ttcgggccgt ccttgaacac gtgtccctgg tgcccgccgc agcgggcaca
     1861 gtggtattcg ctgcgtggca ggatcatctt gaaatccgtt ttggtgccga gcgcatcggg
     1921 cagcggctgc cagaagctcg gccagccggt gccgctgtcg aatttctgtg ccgagtcgaa
     1981 cagcggattg gcgcaggccg cgcagacgaa ggttccatcg cgtttctcgt cgttcagcgg
     2041 gctcgagaag gggcgctcgg tggcctcctg aaagagcacg gcgaagcgct cggacgacag
     2101 gcgctgacgc cattcggcgg cgggtttctt cagttccggc atggcttgct ccacggcgca
     2161 atcgaggacc gcggttggac cggccggtcc cgtgcgcgtt cccacggcgc tcgctggctg
     2221 ccggactcag gtgtcggcca gtgcggcgtg aatttcttcg aaggtcttgg cgacttcgaa
     2281 gctggcgacg gggacgccga ggcggcggcc aatatgggta gccgagaaga tgccgcgcac
     2341 cagatcgaca ccgctgccgg cgtgtttttc ggtgacgatc gcgtgctggc ggccgacgcg
     2401 gcgcagtgtc tcgaggatgt cgccgacctt ggccggcatg acggcctcga tgggcatcgc
     2461 ctgcatgtcc gactgtgcgg tcatgatcag gtcgaccttc aattcgccta cattgccgcc
     2521 cttttcgcgc accgcgcgcg tcgcgcgatc gccggtaagg tcgcgggcgg tgacgacgcc
     2581 gatgatcgcg tcatcgtcat tgaccacgaa caggaaacgg acaccgcgcg agaccatgat
     2641 cgatgcggcc tgggacactg tcgcgtcggg gcggatcatg gccggcgtgg tggtctggaa
     2701 atcggtcatc acgtcgattg ccggcgaatc gagctggacg aggtgatagg tgctgggctg
     2761 gcggcagacg gcgccgatgc gcagcgctga caggtcgatg gcttggtaac ggctcattgt
     2821 cgggtctcct ccttatgtaa ttggaggccg ctgctctgtt cttgttgtgc agcggccggg
     2881 tgaggccggc ggctacttgc cggcgatttc ctcgagcttg ggcgcgcgga tcacctggcc
     2941 gtcgaggccg gcatcctttg ccgagccgcc gtaggcgatg gtcagcaact gcgagtagta
     3001 gagcaccggc atgttgaatt tcgtgccctg cttcttgtta atctctgact ggtaaacctc
     3061 aacgttggcc tggcagagcg ggcagggcgt gacgatcatc tcggcgccgt tgtcgtaggc
     3121 cgactcgacg atgtcgcgaa tctgcttctg cgatttatcg ggctccgaga aggcgaggcc
     3181 gccgccgcag caggtgactt tctggtcgta tttttcaacg gcttcgccgc cgcaactctc
     3241 gaccagcttg tcgaggtatt tcggattctc gaaggattcg ccgtcgatgc cgaaggggcg
     3301 gttggtttgg cagccgacgt agccggcgaa cttgattccc tgcagcggct tgataacagg
     3361 cttggcaagc tcctcgtagc cgaggtcctc gatcagcacc tcgaccatgt gacggacctt
     3421 gacctcgccc ttgtagttga gcccggcggc cgccagtgcc tcgttggttt ccttcatcag
     3481 gacgttcgag ttctcgagac gctccttggc ctcgcgcgtt gccagccagc aggcggcgca
     3541 ggtcgatacc atgtcctgac ccggcaggtg ctgctcggca agcgcgaagt tgcgcgcggt
     3601 gattgccaag cgcggcaatt caccgccctc ggcgtagccg attgaggccc cgcagcagtt
     3661 ccagtcggga atctcggtca tcttgatgtc gagcttgtcg cacatcgagc gggccgaaac
     3721 cagatagttg gaggccgacg ccttcagttg cgaggagcag ccgggataga aggcgatctc
     3781 ttttcttgcc atggtgtttc tccttggggc cgcggcgatt acgccttgag gcccttcttg
     3841 cgatcttcga tctcgcgcgc cttggcgacg atcgcgtgga atccagagag gtcggcgacc
     3901 ttgtgcccgc cgaagatctc catcgggttg aggcgcttcg ccttaagcat gccaaggccg
     3961 atcttctgca tcgacatcgc ggttttcacg cccgatacca ggccatcctt gaagtagtgg
     4021 ccgagcgtca gcgtcagctc gttgacgcgg ccgcgcttgg tcaggttgtc ccagaatagc
     4081 ttggcgaaat cgcgcgtcgg ttggcctttc ggcgcaatac caaggcggtg cgagtaattc
     4141 gccaggccat gcatgatgtg ggtaatcggc aactggcgcg ggcaacgcac catgcagttg
     4201 tagcaggagg tgcacatcca catcgcgtcg gactgcagga cctcgtcgcg cttgccggcg
     4261 cggatcatca tgaagatctt ttgcggggag tgttcccagt gcgggccgaa ggggcaggag
     4321 ccggcacaga cgccgcactg catgcacatc ttgaccatgt ggccttcttc gacgcgctcc
     4381 tcgacttcgc gcaggaagtc gttgcgatac tttgcgacgg cttgggcgtt ggcttgagtc
     4441 atgctcagaa ccccttcatc ggcgacaggc cgagttcctt gatctgttcc gcatactcgt
     4501 tgatcagcgc ggcgcagcgg gcgttgtcgg taatggcgat ctcatgggtg atgacgcgct
     4561 ccttctccag cccgagctgg gtgagcgtgt cgtcgacctt gctcatgcgg tagtgggcca
     4621 tctccgagcc cttgacgaag tggcactggt aatcgtcgcc cttcttgcag cccatcatca
     4681 tgatgccgtc ccagcccgag ttcagcgcgt cggtgaccca gacgagattg accgagccga
     4741 gacagcggac cggaatcgcg cgaatgaagg ctgaatacac atggcgctgc aggccggcca
     4801 tgtcgagcgc cgggtaggcg tcgttctcgc aggcgaggat caggatgcgc ggcttttccg
     4861 agaactcgtc gggcatgtcg aggcacttga tctgtgcgcc gaccgtatcg acgttgtagt
     4921 tctcgaagga gatcacgcgc accgggcagg cacccataca cgtgccacag cggcggcagc
     4981 gcgactcgtt aaacaccggg aagcgctttt cgtcctcgtc gatcgcgccg aacgggcact
     5041 cgaccgtgca gcgcttgcat tgcgtgcagc cctcgaggcg gacgatcggg taggacaggt
     5101 cgcctgagcg cgggtgtgcg gcgcggccga cgctggcgtt ctcgaccgcc tggatcgcct
     5161 ttagcgcggc gccagtcgcg tcttcgcgcg cctgcgccat gtccatcggc cggcgcaccg
     5221 ggccgctggc atagatgccg gtgcgccgcg tctcgtacgg gaagcagatg aagtgcgagt
     5281 cgttgaagcc gtggcggagg attggcagat ccttgccctg gcggtagttg aggttgagga
     5341 tcgagacctt ctctgtgcgc accgggtcat cttcggccag atcgatgttc tgtccgctgt
     5401 tcggcacctg gccggtggcg agcacgacca ggtcggcctc cagcgaagtg tcctcgtcga
     5461 ggatcaggtc gcggaaactc accttggcag cgtcgccggc ggcttcgacg gcgctcgcct
     5521 ttcccttggt gaagatcacg cccttttcct gcgcggcgcg gtagaagtcc tcgcccatgc
     5581 ccggcattcg cagatcctgg tagatcacca cggtgtcgac gtcgggattg tggtccttga
     5641 agtagcgcgc ctgcagcacg ctggtggcgc agcagtggcc cgagcagtag gagagatgca
     5701 tgccggtttc gtcacgctgg ccggcgcatt gcaggaaggc gacgctcttc acctccttgc
     5761 cgtcagcgcg cttgatggcg ccgccattgg cggccgtcgc cagcgcttca agcccggcat
     5821 ggtcgacgac gttggcgagc ttgccgccgc cgagttcggg caacttgtcg atcgcgtagg
     5881 gcgtgaagcc gttcgcctgg atgattgcgc cgacttcctc gctggtggtg ccaccacttt
     5941 cggtggcaat gctcaccgca aagcggcccg gcgcgccgcc ggtctcggcc agcgttgaat
     6001 tgagcagcac cttgatcttc gggtgcttgc cgaccgcctc gatcagttcg gggatgccgg
     6061 ttggctgcgg gtcggcatgg ggctcgcggt cgggaaccgt gcgccaggcc ttgcccgccc
     6121 agccgccgag cgcgccggtc ttctcgacca gcaggacttc gtacccggtg gtcgcgactt
     6181 cgagtgctgc ggtcatgccg gtaaagccgc cgccgacgac caggactcgc ttgttggtcg
     6241 ccgtgttcgg gttgccggca gggatcttca tcttcttcag ttcggcgcag cccattcgca
     6301 cgtagtcgtc ggccatctcc tgacggacct cgtcgtgctc gtcgccctcg gctactaccc
     6361 acaggacgcc ttcacgcaag ttggcgcgcg ccatcgccgc ggtcgggaag tggaaggcct
     6421 cggtcttcgc gcggcgcgaa caggcgccga tcatcacgtg ggtgacgcct tcgttggcga
     6481 tgtcgttctt gatcatcgcc acaccttctt cgccgcagag gaaggcgtgg ttcttcacca
     6541 gcgccatctt gccttccttg gtggcgatct tttccatggt cttgacatcg agcttgtcgc
     6601 cgatgccgca gccggtacag aggtacgcgg cggtcttcat ttcgctcatg cttgggtctc
     6661 cgtcgcggcg gctctatgaa tcacctggat ggcccgcaag gccgccgccg tcgcgctctg
     6721 cgtcgagcgg ttcacgtcga gcggactcga tgcggcgccg gcgccgaaca tgccctgatc
     6781 cttcgaaccc tcgatgaagc ccgacgagtc gaggacgatg tcgtcgggca actggatgcc
     6841 ctgcgtttcc ggctccatcc cgacggcgag tacgacgaga tcgtgcggcg ttgcgtagcg
     6901 gtggtagccc tcggtgtcga cgccgtgcag aaccgggttc tgctccttgt cctcggcaat
     6961 gcgagcaacc ttggacttga tgaagctgac cgtctcgtcc ttctgcacgg cctggtagaa
     7021 gtcctcgaag cggtcgatgg cgcggatgtc gatgtagtag accgtcgact tgcccgagcc
     7081 gtcggggtag gcctcgcgaa cgtaatgggt ctgcttgagc gaggccatgc agcagatgcg
     7141 cgagcagtgg cgcaggtggt tctcgtcgcg cgagccggcg cactggatga aggcgatgtt
     7201 cttcgcctcc ttgccgtcgg aggggcgcaa gagcttgccg ccggtcggcc cgtgcgggtc
     7261 ggcgaggcgc tcgaattcga ggctggtaat gacgttggcg aagcggtcgt agccgtaggg
     7321 ctggatcttc gccgcgtcgt aggggcgcca gccagtcgcc cagaccacgg caccgacgtt
     7381 cagcgtgacg tcctcgtcct tcatgccgag gtcgatcgcg tcgaccttgc aggccgcctt
     7441 ggcagcgtcg ccgcagggcc cgccgacgat gctcgggtcg agcacgtagc gctgcggata
     7501 agccatcgca tgcggcaggt aggcagcctt ggtcttcttc agcttgtact cataggtgtc
     7561 gtcgatctcg gtcgagacgg ccttggcgca gtcgccgcag gccgtgcatt tctcgttgac
     7621 gaaacgcggc gcgacgttca gcgacaccgt gtagttgcca cggctgcctg aaatcccggt
     7681 gacctcggtc atcgtcatca ctcggatgcg gccgttggcc tgctgcgcct tgagtcggcg
     7741 caggttgatc tccagcccgc aggtcgggtg gcacattttt ggaaagtagc ggtagaggcg
     7801 ggaagtacgg ccgccgagcg tcggcagctt ttcgacaagg atcacgtcct tgccgcattc
     7861 ggcggcctcg agggcagcgg tcatgccgct gatgccgcca ccgacgacga gcagggttga
     7921 attggttgcg atggtcccgg tcacatcggt tctccggaag ttgagtcggg cggaagcagg
     7981 tcgtcggcgc tgcgagtccc cttgccgctc gcaacctgcc cagaggcggc tattaaccga
     8041 tattgcagtg caaatcgtct atccccgaga aggggaaagg ggctagcccc aaagggtagt
     8101 tgcggcccga aaaagcctcc gggcaatggt tttcaggccg gcggaacggc gcgcaccgta
     8161 aactcgcagg tggctaggtc gccgagtatc caggtcgagg cgggcgcgcc cagaccggcg
     8221 actgtccccg ggttgaccag ccaggtgtcg gtgccattga cgttgcgcac ggtgcgtacc
     8281 tccggctcgt ggctgtggcc gacgcagacc acgtcgtagc ggccggtcag ggccatcgcc
     8341 tcgccgtatt cggggtagtg gacgacgaac agccggcggc cacctaattc gagcgttgcg
     8401 tcctgcccgt agtaggcaag ccggccgttc atgcgcacgg tcagccggcc gagcgaggcc
     8461 gggtcgccga ggttgttgcc gtggatcacg tgctgcggga tgtcgatcgc cagagaagcc
     8521 ttcagtgtgt tggtgccgat gatgtcgccg cagtggatga cggccgtcgc cccctcggcc
     8581 ttgccggcag cgatggcacg tgccagcgag tcgccacggt cgtggctgtc ggaaacgatg
     8641 cagattttca tgtgtgccta gatgaccagc gggccgtcgt agtcgtcgcc ggtgaccttg
     8701 ggctggacgc gttgcggggc atccccggtg gccgggcgct cggccgcgtg agcggcctga
     8761 acctgcagaa gcgaaacgat gcagtcgccg aggttgtcat agctgtcgat accggtgccg
     8821 aagatttcct cggggccgta gaagaactgg gcgcgatagt cgtcgcgggc ggccttgcag
     8881 accacgaact cggcgccgtc acgctgccag tagaaggcat ggcagtcggt gaggaccggc
     8941 gagggcgggt cgagttgcag ttcgacctcg accgattgga tcggcaccgg ggtgccgtaa
     9001 cgttcgagga gcgtctgctg gaccagcttg cgttcgtgtt cggagaattc aggcgcgttc
     9061 atcggctatt ggcgcaggat atgggcaagg accgcaatct agcgcctgtt ggcgaatcgc
     9121 cgcaactccc gaaatggggg agtcgccccg agttacagac cggaaggcgc gatcacgcta
     9181 gcatcgggcc attccgtttc ttgacttacc gtcggagcat catggaccag aatcacgacc
     9241 ccaagcgcca ttccgccgcc ggtgccgcgc cggcaggcca ggcgctgcgt ggaattccgg
     9301 tccgcgcagc gccggcgcag gtttcgtctt gcgccggcga agaagctttc gcgctacagg
     9361 tacttggcag cgacatggcg cctgagttcg tcgacggcga catcatcatc gtcgaacccg
     9421 acgggctggt cagcgatggc agcttcgtcg tcgcccgcca cgatggtgaa tggctgttcc
     9481 ggcaaatcgt ccggcgcgac gccggctggg tgctggtggc gctggccgat ggcgtgcccg
     9541 agcacgccct gagcgggctc gatgccgtgc gcggcgtgat catcgagaaa acccggcccg
     9601 gacgccgccg tgagcgcaag cgctatgtcg actgaaatgc tcgatgtgct gccacgcgac
     9661 gtgctgccgc gtctgatcga gaccgcacag ttcaacgccg accttgccga tgcgcatttc
     9721 gcgcaggccg cgacgctatg catctacctg ctcgacatgc gcgagtacta ccgctggctc
     9781 aacgccctgc cgctcgatgc gaaactgccg cgcgaacacc tcgggccgtg gatcgccgaa
     9841 caggaagcgc tgtgggatac gctgcgcgcc gacgggcagg aacagcgcga ataccgaccg
     9901 ctcgatccgg cggtcagcgg tgaccccttc gatacccgct cggtccacga gctgctggtg
     9961 agcggtggat tggtctacgg cgcggggatt gggcgcttcg gccggccact gttctttctt
    10021 gccgaatgcc tgcgccgtga aacgcgtgag ggaatcgaca ttgcggtttg cggacgcgag
    10081 ctggcgcgcg gctcgagcgc cccgccggcg atgagccgcg acgcccaggt gatcgtgcgc
    10141 cgggatgctc tcgagcgttg gctgtggaca cgctacgagg agtggcggca tcacccgcgc
    10201 gagaacggct tcgtcgcggc ctggctgctg cacagcggcg gcgttgcccc aggcgaccac
    10261 ccggacccga aggcgatcgt cggccgcata gctgacgccg aaatcgagac gctgatcctc
    10321 cacgagatcg gcgagcgacg gatcgacgcg ctggttggcg atgtctggca cgacatgatc
    10381 gacgacctcg gcaatcgcaa gctcgaggtc ttcgcgcgct cggcgcgcga cctgctggcc
    10441 gattgcgggc atacgctgcc gacgctgctc gaacggggcg acatggcctc gctgcattgc
    10501 tggttcggcc tgctcgacgg catgcggctg aagttctcgc cgacgctggt cgccgcctac
    10561 cgtgccaccg gcggaaaacc cgacgcggcg cttggacgcg tgctcgccga ggcggccgag
    10621 cactggcagg gcgtgtgtac gcagctcgtc gatcactggc agggtggcgg cagcgccgcc
    10681 gtgcaggcgc tgttcgattc gggcgcggtg ggtcactgag gcccgccact gaggcctcgg
    10741 cgccggccgc tataatccgc caccgatcga cgcaccagga gaatccccga tgaagcacct
    10801 gcttcccaaa gaggcctttg agttcctcaa gcagaatccc gacgcgctgt tcatcgacgt
    10861 gcgcagcgag atggaattcc tcttcgtcgg ccatcccaag gatgccctgc atgtggcctg
    10921 gaatgatggc ccggactggg acatcaaccc gcactttgtc ggcgaggtga agaaactcgc
    10981 cggtgcgatg agcgcgcggc ctatggtgct gatctgccgc agcggcaagc gctcggccga
    11041 agccggcgag gcgctcaagg ccgccggctt ccacaacgtc tacaacgtca cccacggctt
    11101 cgagggcgat ctcgacgaga agcatcaccg cggttcggtc aacggctggc gctgcgacgg
    11161 gctgccctgg gagcagtgct gagccgctgc gattgaatgg ctgccggccg cgccgcggcc
    11221 gatagcgtgc ctgcatctcg tgatcgaaat acgccccgcc gcgtttccgc gcgaccttga
    11281 agtcgtccgc gcgttgttcc gtgagtacgc agcggggctc ggcatcgacc tctgttttca
    11341 gggtttcgag gaagaactcg cggcgctgcc cggcaagtac gcgccgcccg gcggacgcct
    11401 gctgctcgcc gaagaagccg gcgaaacgct gggctgtgtc gcgctgcggc cggtcgatgc
    11461 aagcaccggt gaaatgaagc ggctgtacgt tcgcccggca gcgcgtggtc ggcaactagg
    11521 tcgccaactc gccgagcgga tctgcgccga ggcctgcaaa gccggctatt cacgaatctg
    11581 cctcgatacg ctggcgagca tgaatgccgc gctggcgctc tatgccagct taggatttcg
    11641 tccggttgat gcctacgtgt tcaatcccct gcccgacgtg gtctatctcg gccttgcgct
    11701 gaccccggca gctaattgac ccggaagcgg ttgaccaggc tgcgcggatt gccggccgtg
    11761 gatttcagcg agttcgctgc cgttggcgca tcgctcatcg cagcgtggtt ctcgtcgatc
    11821 atgttggcga tcttctcgac attgcgggcg atgtcctggc tcgctgtgcg ctgttcgatc
    11881 accgagagcg aaatttcgtc gatgccgcag ttggcgcagt cgtggctggc ctccagcgag
    11941 cgcagtccgg ccgcaatcgc cgactcgact tgggccgagc gctggcttag cacggcggtc
    12001 acgccgtcga tttcacgggc ggcggcggtc gagcgctcgg cgagcttgcg cacctcgtcg
    12061 gcgacgacca caaagccgcg cccctgctcg ccggcccact ccgcctcgaa gtgctccact
    12121 gctgataggt cagcgttcta gttcgacggt ctgtaaggat tctgctgctg gccatcggca
    12181 gcgacggtct gattgcccag ggaatatgtt gattgctggg ccaatttcgt cagcatttcc
    12241 agcccgagtg aataccggca tagcgccggg aggggcggga atcagcgtaa taccgaatat
    12301 tgtcctgacg aatcgtttgc tacgggcgcg aacggacatg tcattctcga atgtgacctc
    12361 gtcgtgcgtt tcgcaggcgg ggagcagtag cgggatcgga aggccaaaag cgatccagac
    12421 accacaccaa cgtccttttt cctcgtagtg ccagacctcg gtgttgccgt gggtgtcctt
    12481 gcgcagcggc gcgccccagg cgatcaagaa atcgtctttc gttgccggcc gactatcctc
    12541 ggtcggctgg catgcccagc ggtcaaagtc agatttcgca tcaagtggag cacgaagcgg
    12601 aatcggtcgc tcggtcaccg ctgaactggt tcggtcaata aaaattccgg cgcaaccgga
    12661 ggtgcccgtc aggatcgaaa tcagcaagag actcccaaga aatcgccttt ggagttgatt
    12721 tgactctttc aggatagcca tgacgccccc ttcttaacca attggcccag atccttcacg
    12781 gactaggtta atcaggcctc ttccccctga ctatccgaat ctatgagaat tggcgttctg
    12841 gcgggttacg aagaacatcc accgctcagg tagcagcttt ctgcaggggc ataaggtcag
    12901 cagttggccg attgtagcct tccgtttcaa tctaattccg ccaagatagc tgcccgcgca
    12961 gcgccaccgt gtcgccgata atgatcagcg taggcgaggg cggttgcgct gcggcgacgc
    13021 ggtcggcgag tgtttctagc gttgcggcga ccacgatctg gtcgggcagt gtggcgcgag
    13081 caatcagcgc taccggcgtt gtcggtgcgc ggccattggc gatcaactgc cggcagagtt
    13141 cggcgacgcc gggcaaaccc atgtagacga cgacggtctg gcgcggacga accagcgctg
    13201 gccagtcgag atcgagccgg ccgtccttca ggtggccggt gacgaaggcg cagctgtcgg
    13261 cgtgatcgcg gtgggtgagc gggatgccgg cgaagctggc ggcgccggtc gcggcagtga
    13321 tgccggggat gacctggaag cggatgccgt gcgcggcgag cgtggcgatt tcttcgccgc
    13381 cgcggccgaa gacgtagggg tcgccgccct tcagacgcag tacgcggcgg ccggcctgcg
    13441 cgtgttcgac gagcagggcg ttgatgtcag gttgcggcag gctgtgatcg gcgccgcgct
    13501 tgccgacgta aatgcgctcg gcgtcgcggc gcgcgaggtc gacgatggcc ggggcgacga
    13561 gattgtcgta gagcacgacg tcggcctgtt gcatgagccg cagcgccgcg aaggtgagga
    13621 ggtcgggatt gccggggccg gcgccgacca gatagacctc gccgctcgcg ctggtgctgt
    13681 cgccgcgggt ggcggcgatg agcgcggcgt cgagttgttc ctctgcctcg cgctcgcgtc
    13741 cgctgaccgc aagttcggcg accggaccct gcagcgtgct ttcccagaac tgccgccgcc
    13801 gcgattcggg cagcgcggcc ttgacccggt cgcggaatcg gtcggcgagt tcgaccaacc
    13861 ggccaaaggc ggcgggaatc agcgattcga ggcgggcgcg catcagccgc gccagtaccg
    13921 gcgaggcgcc gccagtggac acggcgacga tcatcggcga gcggtcgacg attgccggca
    13981 tgatgaagga gcagagttcg gggtcgtcga cgacgttgac cggaagcttt cgcgcctgcg
    14041 cgtcgcgcga aaccgcggcg ttgacggttc ggtcgttggt cgcagcgatg accagcaccg
    14101 catcgtcgag cgccgcgggt tggtaggcga ctgcgtgatg cgcgatccgg ccggcgttgg
    14161 cttcggcagc cagcgacggg cagagcgccg gcgcgcttac ggtcacccgg gcgccggcgg
    14221 aaagcagcag cgagaccttg cgcgcggcga cctccccgcc gccgaccacg gcgcaggcgc
    14281 ggcccttgag gtcaaggaag atcggcagcc ggtccatgcc tgcagcgcgg tcggtgaacg
    14341 tgctcgcccg atgcctacca gtaggccagc aggcggccct tctcggtctt gctctcccat
    14401 tccttgagcg gcgtatcgcc gaccttggtg caggcgccgg tggtgatgtc gaaggcccac
    14461 ttgtgcttgg gacaggtgag cgtcgtgccc ttcagcgcca ggtgcgggat gttggtggtc
    14521 tggtgcgggc agaggctgtc gtagacgcgg aatttcttct tgccgcggta gatgaagacg
    14581 ctgcggccgt cgcagtcgag gcgtacggtt tcgccgacct tgatgtcgcg cttggcgccg
    14641 aggtcgtgcc agctgccggc ggtggcgccg tcgagcgcga attcgggcag gttcatctgg
    14701 aggatgatgt cggtggccca gacgatcttc gccagcccca gcgccggaaa ggccatcagc
    14761 agtgcgtcga tcacttcctc gccgctggca ccatcgcgca gcgcgcgctt gaggtactgc
    14821 ttgagaccgt tttcggtctg cgcgtgcacc ttggtgatga cggagatcag atttcgcgtt
    14881 ttcggatcga gacgggtgcc ggcgtcctta aggaagctga agtacgcgcc catggcttcg
    14941 ggacgtttct tgacgaggta gttgagcgcg tcgctcatgg gtttggctcc ttggcttggg
    15001 tcttggcggg atcgcttttg cgggtgcagt gtgcgatgaa acgcggcggc tggctactcc
    15061 gaaagcggcg cccggtaacg ccttttggct tattcgctgc gtttgacacg ggtgctagta
    15121 tcttcggcac cgggccgcta cggccattcc ccgaaagagt atccgttccg catgagtgcc
    15181 cgcagccggc tgatcaatcg cgtgtttccg tttatggtct ggcgccaccg ggtgacccgg
    15241 accacgctgc gcgacgacct gatcgccgcg ctgatcggcg ccgtgatcgt gatgccgcag
    15301 ggtgtcgcct ttgccacgct ggcgggcctg ccgccgcagt acggccttta cgcggcgatg
    15361 gtgccggcga ttgtcggcgc gctgttcgga tcgagctggc agctggtctc ggggccgaca
    15421 aacgcgatct cgctggtggt ctttgcgacg ctctcgccgc tggcgattcc cggttcggaa
    15481 aagtacgtgg cgctggcgat tacgctggcc tgcatggttg gcgtcatgca gctggcgatg
    15541 ggcattgcgc gcatgggcgc cgtggtcaat ttcatctcgc acacggtggt cgttgccttt
    15601 acctcgggcg cggcgattct gatcatggcg agccaggtac gtaacttctt cggtattccg
    15661 atcgaagccg gatcgagctt cctgcacacg gttgccgaac tggcgaccca cctcggttcg
    15721 gtcaacctct gggtgctcgc ggtggcgctg acgacgatcc tcgccggcat tctcagccgc
    15781 cggatgtttc cgcgttttcc ctacctgctc tcggcgatgt tcgccggtgc gctgctcggt
    15841 accgcgctca atgccggctt cggcatcgac gagaccggca tccgcacgct cggcgccttg
    15901 cccgccgccc tgccgccgct cagccggccg accttcgacc tcgatacgtg gggccggctg
    15961 gtcggcatcg ccgttgccgt caccgcgctt gggctaaccg aggcggtgtc gatcgcgcgg
    16021 tcgatcgcca ccaagaccgg gcagcgcatc gacggcgaca ccgagttcat cggtcagggg
    16081 atgtccaact tcatcggcag cttcttttcg gcctacccgt cgtcgggttc gttcaaccgc
    16141 tcgggcgcca actacgaggc cggcgccagg accccgctgg cggcggcctt ttccggcgtc
    16201 ttcctcggcc ttgccgtgtt cgtcatcgcg ccgctggcgg cctacctgcc ctacgcggcg
    16261 atggccggca tcctcgtgct ggtggcttgg ggcctgctcg acttccacga gatcatcacc
    16321 atcgcgcgga ccagccgggc cgaggcggcc gtgctcgcgg tcaccttctt cggcacgctg
    16381 gtgatgcacc tagagatggc gatcgtcgcc ggcgtgatgc tttcgctgct gctctacctc
    16441 aaccggaccg cgcggccgac gattcgctcg ctggtgccgg agcccgatac ctcgctcggc
    16501 cgtttcgtcg agcgcaaacc gggcatggcc gagtgtccgc agctgaagat tctgcgcatc
    16561 gagggcgagc tgtacttcgg cgcggtcaac cacgttgccg agtacttcca ctttctcgcc
    16621 gaccaccggc cgggccagaa gcacctgctg ctgatgagcc gcagcatgaa cttcatcgat
    16681 gtcgccggcg ccgaactgct cgcggccgaa gcgcgccgcc ggcgcgatgc gggcggcagc
    16741 ctgtccttcc acggcctgcg cgtcgccgcc ggagacatgc tgtcgcagcc ggcgttcaag
    16801 aacgagttta ccgggcgctt caccggcaag accgaggcga ttgccggctg cttcgcgctc
    16861 ttcgaccgcg acatctgccg gagctgcacg gcgcggatct tccgcgagtg caacagcgtg
    16921 ccatatgagc cagtcgccgc cgcggacgtg ctgccgccgg ccgacgccga tcagaacgga
    16981 aagaagatcg gcagcagcag cgagtagctg gtgagcataa gcacggccag cggcgcgccg
    17041 acccgcaggt agtcgacgaa gcgatagccg cccggggtca tgatcagcag attggtctgg
    17101 taggccattg gcgtcaggta gcagaagttg gcgccgaaca ggatcgccag cacgaagggc
    17161 tcgggcgcaa cgcccagcgt tcgcgcgatg tcgacggcaa tcggcgtgcc gatggcggcc
    17221 gcggcgttgt tcgagacgaa gttggtcagc accagcatca ggctcatcag cacgattacc
    17281 agcaccagtg gcgaagcgcc gtcggcgatt gacagcgccg actgggcgag tgccgccgtg
    17341 gtgccggtct gtaccagcgc gctgccgagc gcgaggctgg atacgacgag caggatgacc
    17401 ttgcccgaga gcgcgttgcc gacgtcgcgc cagccgacgc agcgcgagag gatcatcagc
    17461 gccacgccca tcatcgccgc gacggcgatg ggcaggacct tgaaggcggc gaggacgatg
    17521 gtcagcgcga agatcgccag cgccgctggc gccctctcgg tcgccttgag cgtcagccgc
    17581 ccggagagga ccagcaggcc ggtgcgatcg cgcagttcct ggatgtcgtc ttgcgtgccc
    17641 tggacgagca gaacgtcgcc gacgcgcagc gtgacgtcgg caatgtcctg ctgcgccgag
    17701 atggtatcca tctgcggccg gtgcagggcg acgatgacca cgccgtagtc atcggcgatg
    17761 cgcatcgagc gcaccgtgcg gccgttgagc ggcgagttct cggtgaccac ggcctcggcg
    17821 acctgttgcg gctcgccctt gttctcgacc tcttcctcct cgtcctcagg caattcggcg
    17881 gcgaccgcgc ccagcgtgtc gccgacctcg gcgatcagct tgcctgggcc gacgatctcg
    17941 agccgatcgc cggcgtgcat cttcagcgag ggcagacgcg cgagctcgac gcccgattcg
    18001 cgacggatgc ggacgatccg cccctcctgc ccgaccagct tgcgcagctc ggcgaaggta
    18061 gcgccgtcgg ccggtccgtc tttctcgatg gtcagcttgg tgtggaaaag tcgtggcgct
    18121 gcgtcgccca agggcgtgtc gcgcgttggc agcaggtgcg gggcgatcag ccacaggtag
    18181 aggatgccgc caacggcggc gatcgccgtc aggtggaaaa agtcgaacat gccgaactgg
    18241 cgtacgccca gatcggcggc gatggcgacg acgagcaggt tggtcgaggt gccgatggtc
    18301 gtgcccatgc cgccgatcag caccgcgtag ttggccggca tcagcatctt ccccggcggc
    18361 gtcttgctgc gcagcgccgc gccggtgagg atgggcagca tcagcatca
//
LOCUS       DATDAA010000229         7112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_401769,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000229 DATDAA010000000
VERSION     DATDAA010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401769"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..993
                     /locus_tag="VIS73_07345"
     CDS             160..993
                     /locus_tag="VIS73_07345"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEY5763007.1"
                     /translation="MSSPATGLQFARGVAALFLPLIVPLLHAADNEAMPGSITGAVPQ
                     APQAQAVAAFSAQAPGGALAAPWRLQTYAKVPRHTSFSLIADGGATVLQVRAEQAAAS
                     LIRPFNADPAATPLLRWRWKPLASPQQTALGEKASDDWGARLYVLFEPPPESVSFGER
                     LALGIARAIYGDDLPARGICYVWLPGGRVGAIAANAYTDRLKMIVVDAAPAGSWRAFE
                     RNLADDYRAAFGEALAPVSAIAVSADSDNSGGIAEVLIGDITIGTAPLRAAPDTAPPP
                     R"
     gene            1071..2429
                     /locus_tag="VIS73_07350"
     CDS             1071..2429
                     /locus_tag="VIS73_07350"
                     /inference="COORDINATES: protein motif:HMM:NF017770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HEY5763008.1"
                     /translation="MEATLYATLGVPANATLADIHAAYTRALADLRAQIGQPDEPSPD
                     AVDALRSAYVMLSDPDRRARYDESLAPAAAATAELGLEPIGGAAAPPAAATGPLSGAA
                     AAEQARPHTEKRHWLEFTGDGSSYFRIWIVNLLLSILTLGIYSAWAKVRREQYFHRNL
                     RLAGSAFDYHGEPKAILKGRMIAASFFVVLSLAQHFNPLAYLILILASLPLIPWLLVR
                     TYRFRAHNTSFRGLRFSFDGSYREALTVFLGYGLLTLITLGLCLPLWFRAQRRYIVSH
                     LSFASTRFHNDLGIWPIYRVFLLPMLYAVGAFLLLIFAIGSIAASMNGNVDPAAFVGI
                     AMLGYFAIILVGMLLVGPYLRVRIANLVWNSTTLGIHYTQSVMRFLPYVGIVLGNFVL
                     TVLTLGLYRPWAQVRVARYRAETAALVALAPLDEFIAGEQSKATAVGEEMADMFEFDI
                     AL"
     gene            2426..3448
                     /locus_tag="VIS73_07355"
     CDS             2426..3448
                     /locus_tag="VIS73_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY5763009.1"
                     /translation="MSTPIAVDYYDGHSARRNRARLDYVDGHWRIRGDFGERLLPRAE
                     ATISEPLGQASRSLRWPDGAHCEIADHAGFDQLLQVAGVRATPVVALQSRWLWAIGAL
                     AGFIVIVAFTYVSLLPWAAEHLAQRIPDSAVDSLSREILKSMDQHVLQPSKLDEARQQ
                     ALRERFSALPLPGKAIDHQRVHFRASKRLPPNAFALPSGDIVVFDSLVKLAASDDEVI
                     AVLAHEAGHVAQRHGLRQMLQTTVVSTVITVYFGDISALATGVGALLLESRYSREFER
                     DADDYAADALLAAGKSPELLATMLEKLERSRRGKSADSAPGVDLLATHPDTAERTAAL
                     RARAGR"
     gene            complement(3450..4304)
                     /locus_tag="VIS73_07360"
     CDS             complement(3450..4304)
                     /locus_tag="VIS73_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY5763010.1"
                     /translation="MEGASITIIVLFVFVVITVAKGVRIIPQGEEWIVERLGKYNGTL
                     MPGLRFIVPYVDRVAYKLVTKDIILDVQEQEVITRDNAVIMVNAIAFIKVTDPVKAVY
                     GVVDFSEAIRNLIMTTLRSITGEMELDEALSSREKIKARLRESIADEAVDWGLTVKSV
                     EIQDIKPSPSMQKAMELQAAAERERKATVTRAEGAKQAAILEAEARLEAAKRDAAATV
                     NLAEASAEAIRRVTVAIGDNQTPMLYMLGEKYVTAMQRLGESTNAKLVLLPGDLQEAV
                     KGLLRRAP"
     gene            complement(4332..4775)
                     /locus_tag="VIS73_07365"
     CDS             complement(4332..4775)
                     /locus_tag="VIS73_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY5763011.1"
                     /translation="MIDLAWWHWVVIGLLLIGVELAVPSFYLIWFGLGALVVGLLTAL
                     WPTMGGNLQVIIWTVASVALVVLWFRYWKPRTKSRAGSSDTQMVGEIGLLTRAVEPFV
                     RGEVRFQKPMGGSDLWPCMADEAIAAGERVKVVASEGNFLRVARI"
     gene            4984..5592
                     /locus_tag="VIS73_07370"
     CDS             4984..5592
                     /locus_tag="VIS73_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEY5763012.1"
                     /translation="MTRLHLPLFALALLSIAAPAAYAGSSGGLYIGALAGAASSDDWD
                     RIVGNANGIIGTDATAATSGSLGLSGALGYRLSPGLAVEVSYVDGGTSDVEFATVPAL
                     IEIHKRHAAISVLGILPVSEGIELYGRLGVAHWEMETDLTLAGVKSSDTISGNDLTYG
                     AGLAYELVSQLDLLADYQRIKMKVPSGNLSADYYLAGLRYRF"
     gene            5685..>7112
                     /locus_tag="VIS73_07375"
     CDS             5685..>7112
                     /locus_tag="VIS73_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEY5763013.1"
                     /translation="MSSSARLTASRKQKVMSARAAVDRIPDGATVATGGFVGIGFAEN
                     IAVALEQRFVETAAPKALTLVYAAGQGDGVSRGLNHFGHDGMIKRVVGGHWGLVPTLQ
                     KLAVDNQIEAYNLPQGVITHLFRDIAAGKPGQLSKIGLGTYVDPRHGGGKINAATTED
                     LVKLTEVGGQECLLYRTFPITVGIIRGTTADPDGNVTMEKEALTLEALAIATAAHNCG
                     GIVIVQVERLADRGSLNPRQVKVPGILVDCVVVAEKPEYHMQTFAEQYSPAFSGEIRI
                     PLDTLAPMAMDERKIIARRAAMELVPGAIVNLGIGMPEGVAAVAAEEDLVDLMTLTAE
                     PGVVGGVPASGLNFGAAVNTQAIVDQPYQFDFYDGGGLDLAFLGLAQADAEGNLNVSK
                     FGSRLSGAGGFINISQCARKVVFVGTFTAGGLEVEIIDGRLQIVREGKSPKFVREVEH
                     RTFSGRVAAANRQPVLYVTERCVFEL"
BASE COUNT         1209 a         2505 c         2179 g         1219 t
ORIGIN      
        1 ggcgggacga ttcgatcccg cgcagatcgc cgcgctcgag gcacgcgggc atgtaattcg
       61 cgaattaccg ctgacctcgg gcctgcacgt gattcgccgc gccaccggcg gctggatcgg
      121 cgccgccgac ccccgccgcg aaggcgaggc gcgcggacga tgagttcgcc cgcaactgga
      181 ttgcaatttg cgcgcggcgt cgccgcgctg ttcctccccc tgatcgtgcc gctactgcac
      241 gccgccgaca atgaagcgat gccgggcagc atcaccggag cagtgccgca agcgccgcag
      301 gcgcaggccg tcgcagcgtt ctcggcgcag gcacctggcg gcgcactggc cgcgccctgg
      361 cgcctgcaga cctatgccaa ggtgccgcgg cacaccagct tttccctgat tgccgacggc
      421 ggcgcgacgg tcctgcaagt ccgcgccgag caggccgcgg ccagcctaat ccgcccgttc
      481 aacgccgacc ctgcggcaac gccgctgctg cgctggcgct ggaagccgct ggcatcgccg
      541 cagcaaacgg cgctcggcga gaaagcgtcc gacgactggg gcgcccgcct ctacgtgctg
      601 ttcgaaccgc cgcccgagtc ggtcagcttc ggcgaacggc tcgcgctcgg catcgcccgc
      661 gcgatctacg gcgacgattt gccagcgcgc ggcatttgct acgtctggct gcccggcggc
      721 cgggtcgggg cgatcgccgc caatgcctac accgaccggc tgaagatgat cgtcgtcgac
      781 gctgcaccag ccggcagctg gcgcgcgttc gagcgaaatc tcgccgatga ctaccgtgcc
      841 gccttcggcg aagcgctcgc tccggtctcg gcgatcgccg tctcggccga cagcgataac
      901 agcggtggaa tcgccgaggt gctgatcggc gacatcacga tcggcacggc gcctttgcgc
      961 gccgcgcccg atacggcgcc accgccacga tgacggccac ctgaacatcg ccgataatcc
     1021 aggccgcatc agcgcgaggc cgcccgcaaa gtcaagctca gggatagaac atggaagcca
     1081 ccctctacgc cacgctcggc gttcccgcga acgcgacgct tgccgacatc cacgccgcct
     1141 acacccgagc cctagccgac ctgcgcgcgc agatcggcca gcccgacgaa ccctcgccgg
     1201 acgcggtcga cgcactgcgc agcgcctacg tcatgctcag cgatcccgat cgccgcgccc
     1261 gctacgacga gtcgctggca ccggccgccg ccgccacggc agaactcggc ctggagccga
     1321 tcggcggcgc cgcggcaccg cctgccgcag ccaccggccc cctgtccggt gcggccgccg
     1381 ccgagcaggc acggccgcac accgagaaac gccactggct cgagttcacc ggtgatggca
     1441 gcagctactt ccgcatctgg atcgtcaacc tgctgctgtc gatcctcacg ctcggcatct
     1501 attcggcgtg ggccaaggtc aggcgcgaac agtatttcca tcgcaacctg cggctggccg
     1561 ggtcggcctt cgactatcac ggcgagccga aggcgatcct caaggggcga atgatcgccg
     1621 cctcgttctt cgtcgtgctg agtcttgcgc aacacttcaa cccactcgcc tacctgatcc
     1681 tgatcctggc tagcctgccg ctgatcccct ggcttctggt ccggacctat cgtttccgcg
     1741 cacacaacac cagcttccgc ggcttgcgct tttccttcga tggcagctac cgcgaggcac
     1801 tcacggtctt cctcggctac ggcctgctaa cactgatcac gctcgggttg tgcctaccgc
     1861 tgtggttccg cgcgcagcgg cgctacatcg tcagtcacct gtctttcgcc agcacgcgct
     1921 tccacaacga cctaggcatc tggccgatct atcgcgtctt cctgctgccc atgctctatg
     1981 ccgtcggtgc tttcctcctg ctgatctttg ccatcggtag catcgccgcg tcgatgaatg
     2041 gcaacgtcga cccggccgcc ttcgtcggta tcgcgatgct tggctatttc gcgatcatcc
     2101 tcgtcggcat gctgctggtc ggcccctacc tgcgggtgcg catcgccaac ctggtctgga
     2161 attcgacgac gctgggcatc cattacacgc aaagcgtcat gcgctttctg ccctacgtcg
     2221 gcatcgtcct cggcaacttc gtgctgaccg tgctaacact ggggctttac cgcccctggg
     2281 cgcaggtacg cgtggcacgc taccgcgccg agaccgccgc actggtcgcc ctcgccccgc
     2341 tcgacgagtt catcgccgga gagcagagca aggctaccgc cgtcggcgag gaaatggccg
     2401 acatgttcga attcgacatc gcgctttgag cacaccgatc gccgtcgact actacgacgg
     2461 gcactcggca cgccgcaacc gcgcccgcct cgactacgtc gacggccatt ggcgcatccg
     2521 cggcgacttc ggcgagcgac tcctgccgcg tgccgaagcg acgatatccg agccgctggg
     2581 acaggcctcg cgcagcctgc gctggcctga cggcgcgcac tgcgagattg ccgaccacgc
     2641 cggcttcgat caattgctac aggtagcggg ggtgcgcgca acgccggtcg tcgcgctgca
     2701 gtcgcgctgg ctgtgggcca ttggcgcact cgccggattc atcgtcatcg tggcattcac
     2761 ctacgtcagc ttgctgccct gggccgccga gcacttggca cagcgcattc ccgattcggc
     2821 cgtcgacagc ctgtcgcgcg aaatcctcaa gtcgatggat cagcacgtat tgcagccgtc
     2881 aaaactcgac gaggcgcgcc agcaggcgtt gcgcgagcgc ttctcggcgc tgccgctgcc
     2941 cggcaaggcg atcgaccacc agcgggtcca cttccgcgcc agcaagcgcc tgccaccgaa
     3001 cgcctttgcc ctgccgagcg gcgacatcgt cgtcttcgat tcgttggtca aactcgcggc
     3061 gtccgacgat gaagtcatcg cagtccttgc ccacgaagca ggccacgtcg cgcagcgaca
     3121 cggcctgcgg cagatgctgc agaccaccgt ggtctcgacc gtgattaccg tctacttcgg
     3181 cgacatatcg gcactcgcca ccggcgtcgg cgccttgctg ctcgaatcgc gctactcgcg
     3241 cgaattcgag cgcgatgccg acgactacgc tgccgacgcg ttgctggctg cggggaaatc
     3301 gcccgagttg ctggcgacga tgctagaaaa gctggagcgg agccggcgcg gcaaaagcgc
     3361 cgattccgcg cccggggtag acctgctagc gacgcacccc gacacggccg agcgcactgc
     3421 tgcgctgcgc gcccgcgccg gacgatagct cagggggcgc ggcgcagcag tcccttcacc
     3481 gcctcctgca ggtcgccggg cagcagcacg agcttggcgt tggtcgattc gccgagtcgc
     3541 tgcatggcgg tgacgtattt ctcgccgagc atgtagagca tcggcgtctg gttgtcgccg
     3601 atcgccaccg tcacccggcg aatcgcttcg gctgacgctt cggcgagatt gaccgttgcc
     3661 gccgcgtcgc gcttggccgc ctcgaggcgc gcctcggcct cgaggatcgc cgcctgcttc
     3721 gcgccctcgg cgcgcgtaac ggtcgccttc cgttcgcgtt ccgcagcggc ctgcagttcc
     3781 atcgccttct gcatcgacgg cgagggcttg atgtcctgga tctcgaccga cttcaccgtc
     3841 agcccccagt ccaccgcctc gtcggcgatc gattcgcgca accgggcctt gatcttctcg
     3901 cgcgatgaaa gcgcctcgtc gagttccatc tcgccggtga tcgagcgcag cgtggtcatg
     3961 atcagattgc ggatggcctc ggaaaaatcg acgacgccat agaccgcctt gaccggatcg
     4021 gtgaccttga tgaaggcgat cgcgttgacc atgatcaccg cgttgtcgcg agtgatcacc
     4081 tcctgctcct ggacgtcgag gatgatgtcc ttggtcacca gcttgtaggc gacacggtcg
     4141 acatagggga cgatgaagcg caggcccggc atcagcgtgc cgttgtactt gccgagccgc
     4201 tcgacgatcc actcctcgcc ctgcgggatg atgcggacgc ccttggcgac cgtgatcacc
     4261 acgaagacaa agaggacgat gattgtgatg ctggcgcctt ccatcatttg cttcccttga
     4321 aatgaaatcg ttcagattcg cgcaacgcgc aggaaattgc cttcggaagc gacaaccttg
     4381 acccgctcgc cggcggcaat cgcctcgtcg gccatgcagg gccagagatc ggaaccgccc
     4441 attggcttct ggaatcgcac ctcgccacgg acgaagggct cgacggcgcg tgtcagcagg
     4501 ccgatctcgc cgaccatctg cgtatccgac gaccccgcgc ggcttttcgt acgcggcttc
     4561 cagtagcgaa accagagcac caccagcgcc accgaggcga cggtccagat gatgacctgc
     4621 aggttgccgc ccatcgtcgg ccacagcgcg gtcaacagac cgacgaccag cgccccgagc
     4681 ccgaaccaga tcaggtagaa cgacggcact gccagttcta cgccaatcag caacaggcca
     4741 atcaccaccc aatgccacca agccagatcg atcacgacgc acccctgcta gaagttgcgc
     4801 aaggatagca actgtcggtg caacgctcgc ggagtatttt ccggcgcttc gtcgatgtca
     4861 taccgggtat cgtcgaatcc tgataggatt tcgcgcgttt cacgggccga aagccgatta
     4921 ccgcacgttt tcgctatgcc ttcgcgaccc gtctacagca accgaaccgg acgacaacaa
     4981 accatgaccc gcctgcacct ccctctgttt gccctcgccc tgctctcgat cgccgctccg
     5041 gcggcctacg ccggttcatc aggcggcctg tatatcggcg ctctggctgg cgctgcctcg
     5101 tccgacgact gggaccggat cgtcggcaac gccaacggaa tcatcggcac cgatgcgacc
     5161 gcagcgacga gtggttcctt gggcctcagc ggcgcactcg gatatcggct gagccccgga
     5221 ctggcggtcg aggtgagtta cgtcgacggc ggcacctccg acgtcgaatt cgccacggtc
     5281 ccggcgttga tcgagatcca caagcggcat gcggcgatat cggtacttgg catcctgccg
     5341 gtctcagaag gaatcgagct gtacggccgg ctcggtgtcg cccactggga aatggaaacc
     5401 gacctgacgc tggccggcgt caagtcctcc gacacgatta gcggcaacga tctgacctac
     5461 ggtgccggac tagcctacga gctggtgtcg caactcgacc tgctcgccga ttaccaacgg
     5521 atcaagatga aggtgcccag cggcaacctg tcggccgatt actacctggc ggggctgcgc
     5581 taccgcttct gatccgtggg accagtcgcc gcaaccccat cactgcgcct gtagcgcgcc
     5641 ggtagaatgc gtggcgatca ggccacaaca ttcttgaggt gcgaatgagt tcaagcgcga
     5701 ggcttacggc cagccgcaaa cagaaggtca tgtcagcgcg cgccgccgtt gaccggattc
     5761 ccgacggcgc caccgtcgcc accggcggct tcgtcggtat cggtttcgcc gagaacatcg
     5821 ccgttgccct cgaacagcgt tttgtagaga ccgctgcacc caaggcgctg acgctggtct
     5881 acgcggccgg ccagggcgat ggcgtgtcgc gcgggctcaa ccacttcggc cacgacggca
     5941 tgatcaaacg cgtcgtcggt ggccactggg gtttggtgcc gacgctgcag aagctcgccg
     6001 tcgacaacca aatcgaagcc tacaacctgc cgcagggcgt gatcacgcac ctgttccgcg
     6061 acattgccgc gggcaagccg gggcagctat cgaagatcgg ccttggcacc tacgtcgacc
     6121 cacgccacgg cggcggaaag atcaatgcgg cgaccaccga ggatctggtg aagctgaccg
     6181 aggtcggcgg ccaggagtgc ctgctctacc ggaccttccc gatcaccgtc ggcatcatcc
     6241 gcggcaccac ggccgatccc gacggcaacg tgacgatgga aaaggaggcg ctgacgctcg
     6301 aggcgctcgc catcgccacc gccgcgcaca actgcggcgg catcgtcatc gtccaggtcg
     6361 aacggctcgc cgaccgcggc tcgctgaatc cgcgtcaggt caaagttccg ggcattctcg
     6421 tcgattgcgt ggtggtcgcc gagaaacccg aataccacat gcagaccttt gccgagcagt
     6481 acagcccggc cttctcgggc gagatccgca ttccgctcga tacgctggcg ccgatggcga
     6541 tggacgagcg caagatcatc gcccgccggg cagcgatgga gctggttccc ggcgccatcg
     6601 ttaacctcgg gatcggcatg cccgagggcg tcgccgccgt cgccgccgaa gaagacctcg
     6661 tcgacctgat gacgctgacc gccgaacccg gcgtggtcgg cggcgtgccg gcctcgggcc
     6721 tgaatttcgg cgctgcggtg aatacgcagg cgatcgtcga ccagccctac cagttcgact
     6781 tctacgacgg cggcggtctc gacctcgcct tcctcggcct cgcgcaggcc gatgccgaag
     6841 gcaatctcaa cgtctcgaaa tttggctcgc gcctctcggg cgccggtggc ttcatcaaca
     6901 tctcgcagtg tgcgcgcaag gtggtcttcg tcggcacctt cactgccggc ggactggaag
     6961 tcgagatcat cgacggcagg ctgcagatcg ttcgcgaggg gaaatcgccg aagttcgtgc
     7021 gcgaggtcga gcaccgcacc tttagcggcc gggtcgcagc ggcgaaccgg cagccggtgc
     7081 tctacgtcac cgaacgctgc gtctttgaac tg
//
LOCUS       DATDAA010000230        11691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_227170,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000230 DATDAA010000000
VERSION     DATDAA010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11691
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227170"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1040
                     /locus_tag="VIS73_07380"
     CDS             255..1040
                     /locus_tag="VIS73_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763014.1"
                     /translation="MRAVFPSRIDRSERMSSRLAITIAVLAFSAGQGLASAAEALITP
                     DIEVGEREIEVGIGIADEDGERNSGLAIGFGAGIANRWASEIGVEFEKEDGDDFEFSG
                     VEWENRIGLIVDEDEPMALSLLVGFERPRERAEGWSSTLGLLSESTPGRFLVNANLLL
                     TRTWDEADSGGEEPGGTTLGYQWRSLYRYSHRIYYGLWGMGDLGRWNDWADKEEQSHQ
                     LGPAIYGRLKLGGQRLNYDVGLLFGLTDGAPERGLRLKLEYEF"
     gene            complement(1072..1794)
                     /locus_tag="VIS73_07385"
     CDS             complement(1072..1794)
                     /locus_tag="VIS73_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5763015.1"
                     /translation="MFPLRLDCVGFHPDGRPVLDEVDLTLDGDGITVLLGPNGAGKSV
                     LLRVIAGLQLPDSGSIDWAGDRAPSPGRISMVFQNPLLIRASVADNVALSLRPLGVGM
                     AERARRIDDALARVGLSHRRTDAARLLSGGEKQRLALARCWASRPQLLILDEPTASLD
                     PTATDAVETIIREIRTDGAKILMTTHNLAQASRLADDVVFIDRGRVCEHAPAQRFFAH
                     PQSAEGRLYLQGELPWRITFDD"
     gene            complement(1800..2495)
                     /locus_tag="VIS73_07390"
     CDS             complement(1800..2495)
                     /locus_tag="VIS73_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5763016.1"
                     /translation="MSSTLLDALGLLTSFDAAVAQVVALSLFVSGSAVALGVFIGLPL
                     GAFVAIARFPGRAAVTVLLNAMMGLPSVVVGVIVYLALSRSGPFGPLGLLFTPTAMII
                     AQTLLTAPLIAALTRQIVEDSWRHYETELNSMGYGRWQAVVVLLYDCRFSLVVAVLAG
                     LGRAMSEVGAVMMVGGNIAGFTRVMTTTIALETSKGDLPLSLALGAVLVALVLGLNAA
                     AHGLRQWAMRRYG"
     gene            complement(2609..2896)
                     /locus_tag="VIS73_07395"
     CDS             complement(2609..2896)
                     /locus_tag="VIS73_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763017.1"
                     /translation="MPATHFERLISASVDEFVRGVRAAFPDAAQVDGTRCTIAVPPVT
                     LAITLTPLAPRRIALLALPQLNAAFAFEGGSAAEQARLLEHLDRSMHRGGG"
     gene            complement(2909..3160)
                     /locus_tag="VIS73_07400"
     CDS             complement(2909..3160)
                     /locus_tag="VIS73_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY5763018.1"
                     /translation="MKITFKLFATLTDYLPAGHQGNVIEIEVPDDTTVGSVIAQHHLP
                     EKLTHLVLVNGNFIAPEKRANEPLKPGDTLAIWPPIAGG"
     gene            complement(3251..4501)
                     /locus_tag="VIS73_07405"
     CDS             complement(3251..4501)
                     /locus_tag="VIS73_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5763019.1"
                     /translation="MKHVILGNGPAGVVAAETLRSADPKAEITLVGCEDAPPYSRMAI
                     PYLLTGKIDERGTYLRKSDSHYADLNIADVRAKATKLDAKAKTVALDNGKSLAYDRLL
                     VATGSHPIRPPIPGLDLPGVHPCWTLEDARAIQKLAKPGARVLQIGAGFIGCIILEAL
                     VKRGVKLTVVEMGDRMVPRMMTQVAGNMIRKWVEEKGVAVVTGAKVDKIEKGSPLKAT
                     LSTGKVLDCDLIIASAGVAPNVDFLDGSGVKVERGIVVDATMQTSVPGIYAAGDVVET
                     FEFDTGKPFVNAIQPDAVDQARVAALNMAGKATGSQGSLAINVLDTIGLISASFGQWQ
                     GVAGGDGVEHANAADYRYLSLQFKGDRLVGATTIGTTEHVGALRGLIQNRTRLGHWKQ
                     TLLANPTRFVEAYVASSQPVSIGR"
     gene            complement(4568..6415)
                     /locus_tag="VIS73_07410"
     CDS             complement(4568..6415)
                     /locus_tag="VIS73_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY5763020.1"
                     /translation="MAWARKILRVDLAAGTCKSEDLNMKLADEFLGSRGLGTKYFCDE
                     VDPKVDPLSPANKLIFATGPLTGTMASTGGRSTVITKGPLTGAIACSNTGGFFGAELK
                     FAGWDMIIVEGKSAKPVYLSIENQKAELKDASHLWGKSTWDTEAMIKSAHQDPQTRVA
                     SIGRSGENGVLYACVVNDLHRAAGRSGVGAVMGSKNLKAVAVRGTKGVSGIKDFKAFL
                     DATQAGKQVLKDNAVTGQGLPKYGTQVLMNVINEVGALPTRNHRDVQFEEASKISAEA
                     MHETRKTDGKTHLITNAACFGCTIACGRISQLDQNHFTVQNSPKYWGASGGLEYESAW
                     SLGASNGVGDLEALQYANMVCNEDGFDPITFGTTVSAVMELYEMGVLTKEQIGIEAPF
                     GSAKALAYMAEITARGEGFGKDIGLGSKRLCEKYGHPELSMSVKGQEFPAYDGRALQG
                     IGLNYATSNRGACHVRGYTVASEVLGIPVKTEPAATEGKPGLVKAFQDATAVFDSSGL
                     CLFTSFAWTLADIQPQIQAACDGDWSLDKLNLVGERIWNLEKQFNLKAGFTRKDDTLP
                     PRLLKEGCKTGPQKGNVAEIGKMLPEYYELRGWDKEGVPTADTVKRLGL"
     gene            complement(6428..6898)
                     /locus_tag="VIS73_07415"
     CDS             complement(6428..6898)
                     /locus_tag="VIS73_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY5763021.1"
                     /translation="MMKSLYLDPKKCTGCLQCEMACSYEHTGAINPSMSRIKVFDFEH
                     EGRKVPYTCTQCAEAWCMSACPVEAIKIDHETGAKMVLEATCVGCKVCTIACPFGTIN
                     YVKQTGKVQKCDLCGGDPECVKACPTGAITYVDANWTGLDKMRAWAAKANAAAL"
     gene            7372..8628
                     /gene="glp"
                     /locus_tag="VIS73_07420"
     CDS             7372..8628
                     /gene="glp"
                     /locus_tag="VIS73_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY5763022.1"
                     /translation="MSNSSSCAAPESGATLTVAAAQRQILDTLQPISGYEAVAVRDAF
                     GRVLARDVVAPFDVPAHDNSAMDGFAVRAEDLSAERPTTLQVVGTALAGGGFSGIVGS
                     GQAVRVMTGAVLPRGADVVVVQEVVREAGERGVEIPTGQRTGQNIRRAGEDIARGNVA
                     LPAGKRVGAAELGLLASLGIAEVAVVRRLRVAFLSTGDELASIGRSLAPGEVYDSNRY
                     TLHGALRRLDVDLIDMGVVRDEPAAMESAFSLAAAQADVVITTGGVSVGEADFVKAMV
                     ARLGEVRFWKIAIKPGRPMAFGRIGDAWLFGLPGNPVAVMVAFYLFVADALRKLSGQD
                     PLPVHPTLRAPCAGAIRKMKGRREFVRGILFEGDGGWQVRPGPLQGSGVLSSMSSANC
                     FIVLPEEGSDVAPGDLVEVQPFEGLW"
     gene            8639..9445
                     /gene="moeB"
                     /locus_tag="VIS73_07425"
     CDS             8639..9445
                     /gene="moeB"
                     /locus_tag="VIS73_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY5763023.1"
                     /translation="MNDEQLLRYSRHIMLAPIDVDGQQRLIDGHALIIGAGGLGAPAA
                     LYVASAGVGRITICDGDTVDLTNLQRQVIHRESTIGVNKALSAQRAIADINHDCRVIP
                     VPKRVGDDELAALVADADVVLDCSDNFATRHAVNVACVERRKPLVSGAAIRFSGQVVV
                     FDPRRDDAPCYECLFPAGGGDVEERCAVMGVFAPLTGIIGTLQAAEAIRLLLGLDGGT
                     DGELVLFDAQSMDWQRMRFRKDPACRVCGSGHANHHEAVLGAPQAGDFRI"
     gene            9520..11010
                     /locus_tag="VIS73_07430"
     CDS             9520..11010
                     /locus_tag="VIS73_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5763024.1"
                     /translation="MRVLLIYSNQSRELVPAPPVGVSYVASASEAAGHSTRLLDLAFS
                     RDVAADIRTAIRALRPEVVGLSIRNIDNLVQQRFESPQAALLAQIAVIRREARRADGT
                     PVPLVLGGPAVSVLGEAAITIFDADFAVIGEGETSFPQLLSALEAGDSAALAAVEGLC
                     WRREGEVVVNPVGRLGQFAGSGMERWVDWDAYQRQGGTWPIQTKRGCPMRCSYCSYPL
                     IEGRRGRLREPAEVVDEIERVLRETSCNGGVRPRTFEFVDSTFNVPASHAIAICEEII
                     RRRVGTSFTAMGINPRDVPPELFPIMKRAGFNSVMITPESASPAMLENYVKGFTMAEV
                     EQTLQHVRAAKLKSMWFFMLGGPGETMETCEESIAFARDRLRSRYFFTVFFTGIRVLP
                     GTLLARQAIERGHLDPAADLSGGVFYLSPAVDEQRVIDRINAAIVRNPAIVHAAEGGS
                     RSQPPLYRALNALRVAPPYWRFLPEFLGFPPLHFLRSRNPSIRAAG"
     gene            11087..11347
                     /locus_tag="VIS73_07435"
     CDS             11087..11347
                     /locus_tag="VIS73_07435"
                     /inference="COORDINATES: protein motif:HMM:NF028001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5062 family protein"
                     /protein_id="HEY5763025.1"
                     /translation="MKKLKHENELFKAALQAGVEYAEGRGVVEFEPTDSAKEKLLYIY
                     RLLVHDKVISPMPEEQVSEKTLRHRVSMWYAHQLPKDHPLLQ"
     gene            complement(11387..>11691)
                     /locus_tag="VIS73_07440"
     CDS             complement(11387..>11691)
                     /locus_tag="VIS73_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763026.1"
                     /translation="EGGRIYLHNLYVIPEIGGTEQVVPLFNGVMAQVAEGVAQMSICQ
                     DNVIAVADVYSAFFGQSGLLLIERFEKKGFLNTFEVHPTNKGHRVIEDLFRRLDLR"
BASE COUNT         1916 a         3679 c         3943 g         2153 t
ORIGIN      
        1 accgagcagc acgctgtccc cagcagtagt gagcaaagaa ggcgacgcat cgttggatct
       61 cccatcgggc tgggcggtgg ttgactgtct gtgttgtgca gcaagtttcg tgcgtgccgg
      121 atctcctgaa acgagacggt caaccgctgc ccttgatgga gacgacgctg gtacgtgaat
      181 gcggtgtgcg agtcggatga tgccacaggg gtttgcactg cctgcattgg caggattgaa
      241 cggtggcatg cagattgcgg gcggtttttc catcacggat cgacaggagt gagcggatga
      301 gttctcgact tgccatcacc attgcggtgc tcgctttttc tgcaggccag ggtctggcct
      361 cggctgcaga ggcgctgatc acgccggata tcgaagtcgg agaacgcgaa atcgaggtgg
      421 gtatcggcat tgcagacgaa gacggcgagc gcaatagcgg tctggcgatc ggcttcggcg
      481 caggcattgc caatcgctgg gccagcgaga ttggcgtcga gttcgagaag gaggatggcg
      541 acgacttcga attcagcggc gttgaatggg agaaccggat cggcctgatc gtcgacgagg
      601 acgagcccat ggcgttgagc ctgctcgtcg gttttgagcg gccgcgcgag cgcgccgaag
      661 ggtggtcgtc aacgctcggg ctgttgtccg agtcgacgcc cgggcggttt ctggtcaacg
      721 ccaacctgct gctcacgcgg acctgggatg aggccgactc gggcggcgag gagcccggcg
      781 gcacgacgct tggataccaa tggcgatcgt tgtatcgcta ctcgcaccgc atctactacg
      841 gcctgtgggg catgggcgat cttgggcggt ggaacgattg ggctgacaag gaagagcagt
      901 ctcaccagct tgggccggcg atctacggac gcctgaagct gggtgggcag cgtctcaact
      961 acgacgttgg cctgctgttc ggactgaccg acggcgcgcc ggagcgcggg ttacgcttga
     1021 agctggaata cgagttctga ccggttgaga caacgtatcg cgccggcgtc gtcaatcgtc
     1081 gaaggtgatt cgccacggta gttctccctg cagatagagc ctgccttcgg cgctttgcgg
     1141 gtgagcgaag aagcgttgtg ccggggcgtg ctcgcaaacg cgtccgcggt cgatgaatac
     1201 gacatcatcg gccagccgcg atgcctgtgc caggttgtgc gtcgtcatca ggatcttcgc
     1261 accgtcggta cgaatttccc ggataatggt ttcgacggca tcggttgccg ttgggtcgag
     1321 gctcgcggtc ggctcgtcga gtatcagcag ttgcggtctg gatgcccagc atcgggcgag
     1381 cgccaggcgc tgtttttcgc caccggagag aaggcgagcc gcatctgtac gccgatgtga
     1441 caatccaacc cgcgcgagtg cgtcatcgat tcgtcttgca cgctcggcca tcccgactcc
     1501 gagcggtcga aggctgagcg ccacattgtc tgcaaccgag gcgcggatca gcagggggtt
     1561 ctggaacacc atgctgatcc ggcccggcga tggcgcgcgg tcgcctgccc agtcgattga
     1621 gccgctatcc ggaagctgca ggccggcgat gacgcgcaac agcaccgact tgccggcgcc
     1681 gttggggccg agcaggaccg taatgccatc gccatcgagc gtcagatcga cctcgtcgag
     1741 gactggacgc ccatccggat ggaagccgac gcagtcgagg cgaaggggaa acatcgcgtt
     1801 catccgtagc gccgcatcgc ccactggcgc aagccgtgtg ccgctgcatt gaggcccagc
     1861 accagtgcaa cgaggacggc gccgagcgcc agcgacagcg gcagatcgcc cttgctggtc
     1921 tcgagcgcga tggtcgtggt cataacccgc gtgaagccag caatgttgcc gccgaccatc
     1981 atcaccgcgc cgacctcgga catcgcgcgg ccaaggccgg cgaggaccgc caccaccagc
     2041 gagaaccggc agtcgtagag cagcacgacc accgcctgcc agcggccgta gcccatcgag
     2101 ttgagttcgg tttcgtagtg tcgccagctg tcctcgacga tctgccgggt gagcgcggcg
     2161 atcagtgggg cggtcagcag cgtctgcgcg atgatcattg ccgtcggcgt aaacagcagg
     2221 ccgagcggac cgaacgggcc ggaacgggag agcgcgaggt agacgatgac gccgacgact
     2281 accgacggca gtcccatcat cgcgttgagc aggaccgtga ccgcggcccg cccggggaag
     2341 cgggcgatgg cgacgaaggc gccgagaggc aggccgatga aaacgccgag cgccaccgcg
     2401 ctgccgctga cgaacagcga gagcgcgacg acctgggcga ccgcggcatc gaagctggtc
     2461 agcaggccaa gcgcgtcaag cagggtggaa ctcacggctt ggcccccggc gcagccccct
     2521 tcgcgtggga ggcgacggca agcggtcgcg cgggcgccac ttcggagcgc gacgggtcgc
     2581 cgcgcggacg ggcgtgccgc acgtccactc agccgccgcc gcggtgcatg ctgcgatcga
     2641 gatgttccag cagacgcgcc tgctctgccg cgctgccgcc ctcgaaggcg aaggcggcgt
     2701 tcagctgggg cagggcgagc agagcgattc gtcggggcgc caggggggtc agcgtgatcg
     2761 ccagtgtcac cggcggtacg gcgatcgtgc agcgcgtgcc gtcaacctgc gcggcatcgg
     2821 gaaaggccgc gcgcacgcca cgtacgaact cgtcgacgct ggcgctgatc aggcgttcga
     2881 aatgcgtcgc gggcatcacc gaaggagctc agccgccggc gatgggcggc cagatcgcca
     2941 gcgtgtcgcc gggtttcagc ggctcgttgg cgcgcttttc gggcgcgatg aagttgccgt
     3001 tgaccaggac caggtgggtg agcttctcgg gcaggtgatg ctgcgcgatg acgctgccga
     3061 ccgtcgtgtc gtcggggacc tcgatctcga ttacgtttcc ctgatggccg gccggcaggt
     3121 agtcggtgag cgtggcgaag agcttgaagg tgattttcat gtagggagtg taggccagca
     3181 gagcaagttt gtgccgcgag actgtgccgg gtcgcaaaac ggcccggcac agtccggggt
     3241 gtcgccgatg tcagcgacca atcgacaccg gctggcttga tgcgacgtag gcctcgacga
     3301 atcgcgttgg gtttgccagc agcgtctgct tccagtgtcc cagtcgcgtg cggttctgga
     3361 tcagaccgcg cagtgcaccg acatgttccg ttgtgccgat ggtcgtcgcg ccgaccaggc
     3421 ggtctccctt gaactgcagg ctgagataac ggtagtcggc cgcattcgcg tgctcgacgc
     3481 cgtcgccacc ggcgacgccc tgccactgcc cgaaggatgc cgagatcagg ccgatcgtgt
     3541 cgagcacgtt gatcgcgagc gacccctgcg aaccggtggc cttgcccgcc atgttcagcg
     3601 ccgcgacgcg cgcctgatcg accgcatcgg gctggatcgc gttgacaaat ggcttgccgg
     3661 tatcgaactc gaaggtctcg accacatctc ctgcagcgta gatgccggga accgaggtct
     3721 gcatggtcgc gtcgacgacg atgccgcgct cgaccttgac gcctgagccg tcgaggaagt
     3781 cgacgttggg cgcgacgcct gccgaggcga tgattaaatc gcaatcaagg accttgccgg
     3841 tcgacagcgt cgccttaagc ggcgatcctt tctcgatctt gtcgaccttg gcgccggtga
     3901 ctacggcgac gcccttttcc tcgacccact tgcggatcat gttgccggcg acctgggtca
     3961 tcatgcgcgg caccatgcgg tcgcccatct cgaccaccgt cagtttgacc ccgcgcttga
     4021 ccagcgcctc gaggatgatg cagccgatga agccggcgcc gatctgcagc acgcgcgcac
     4081 cgggtttggc gagtttttgg atcgcgcgcg cgtcctcgag cgtccagcac gggtgtacgc
     4141 cgggcaggtc taggccggga atcggtggcc ggatcgggtg cgaaccggtg gcgacgagca
     4201 atcggtcgta ggcgagcgac ttgccgttgt ccaacgcgac ggtcttcgcc ttggcgtcga
     4261 gcttggtcgc cttggcacgc acatcggcaa tgttcaggtc cgcgtaatgc gagtcggatt
     4321 tgcgcaggta ggtgccgcgc tcgtcgatct tgccagtcag caggtaagga atggccatgc
     4381 gcgagtaggg tggggcgtcc tcgcagccga ccagcgtgat ctcggccttg gggtcggcag
     4441 aacgcagcgt ttcggcggcg accacgccgg ccgggccatt gcccagaatg acatgcttca
     4501 ttttcgcgag tctccaaaaa aagcggggtc gcgtctgtcg cgcgaccccg gttcgatcag
     4561 ctccggctca gagcccgagg cgcttgaccg tgtcggccgt cgggacgcct tccttgtccc
     4621 agccgcgcag ctcgtagtac tcgggcagca tcttgccgat ctcggcgacg ttgcctttct
     4681 gcgggccggt cttgcagcct tccttgagca ggcgcggcgg gagcgtgtcg tccttccggg
     4741 tgaagccagc cttcaggttg aactgcttct cgaggttcca gatccgctcg ccgacgaggt
     4801 tcagcttgtc gagcgaccag tcgccgtcgc aggcagcctg aatctgaggc tggatgtcgg
     4861 cgagcgtcca cgcgaacgag gtgaacaggc acagacccga cgaatcgaag accgccgtgg
     4921 cgtcctggaa ggccttgacc aaccccggct tgccttcggt ggcggcgggt tcggtcttga
     4981 ccggaatgcc cagcacctct gaggcaaccg tgtagccgcg cacgtggcag gccccgcggt
     5041 tcgaggtggc gtagttcagg ccgatgccct gcagcgcacg gccgtcgtag gccggaaact
     5101 cctgaccctt gacgctcatc gacagctcgg ggtggccgta cttttcgcac agccgcttcg
     5161 agccgaggcc gatgtccttg ccgaaaccct cgccgcgggc ggtgatctcg gccatatagg
     5221 cgagcgcctt ggccgagccg aagggcgcct cgatgccgat ctgctccttg gtcagcacgc
     5281 ccatctcgta gagctccatc accgccgaca cggtggtgcc gaaggtgatc gggtcgaagc
     5341 cgtcttcgtt gcagaccatg ttggcgtatt gcaatgcttc aagatcgccg acgccgttcg
     5401 acgccccgag cgaccaggcc gattcgtact cgaggccgcc ggaggcgccc cagtacttcg
     5461 ggctgttctg cacggtgaag tgattctgat cgagttgcga gatgcgcccg caggcgatcg
     5521 tgcagccaaa gcaggcggcg ttggtaatga ggtgcgtctt gccgtcggtc tttcgcgttt
     5581 cgtgcatcgc ctcggccgag atcttcgagg cctcctcgaa ctgtacgtcg cggtgattgc
     5641 gggtgggcag cgcgccaacc tcgttgatca cattcatcag cacctgcgta ccgtactttg
     5701 gcaggccctg accggtgacc gcgttgtctt tcagcacctg cttgccggcc tgggtggcgt
     5761 caaggaaggc cttgaagtcc ttgatgccgg agactccctt ggtgccgcgc acggcgaccg
     5821 ccttgaggtt cttcgagccc atcacggcgc cgacgcccga acggccggca gcgcgatgca
     5881 gatcgttgac gacgcaggcg tagagcacgc cattctcgcc cgatcggccg atcgacgcga
     5941 cgcgggtttg cgggtcctga tgcgcggact tgatcatcgc ctcggtgtcc caggtactct
     6001 tgccccagag gtgcgacgcg tccttgagtt cggccttctg gttctcgatc gagaggtata
     6061 ccggtttcgc cgacttgcct tcgacgatga tcatgtccca gccggcgaac ttcagctcgg
     6121 cgccgaagaa gccgccggtg ttcgagcagg cgatcgcgcc ggtcagcggc cccttggtga
     6181 tcaccgtcga acggccgccg gtcgaagcca tcgtgccggt cagcggcccg gtggcgaaga
     6241 tcagtttgtt cgccggcgac agcgggtcga ccttgggatc gacttcgtcg cagaagtact
     6301 tggtgcccag accgcgcgag ccgaggaact cgtcggccag cttcatgttg agatcttcgc
     6361 tcttgcaggt gccggccgcg aggtcgacac gcagaatctt tctagcccat gccatgtcgt
     6421 tttctcctta gagcgccgcg gcgttggctt tggcagccca cgcacgcatc ttgtcgagtc
     6481 cggtccagtt ggcatcgacg taggtgatcg cgccggtcgg acaggccttg acgcattccg
     6541 ggtcgccgcc gcagaggtcg cacttctgca ccttgccggt ctgcttgacg tagttgatcg
     6601 tgccgaaggg gcaggcgatc gtgcacacct tgcagccgac gcaagtcgcc tcgagcacca
     6661 tcttcgcgcc ggtttcatgg tcgatcttga tggcctcgac cgggcaggcc gacatgcacc
     6721 aggcttcggc gcactgcgtg caggtatagg gaaccttgcg accttcgtgt tcgaagtcga
     6781 agaccttgat ccgtgacatc gagggattga tggcgccggt gtgttcgtag gaacaggcca
     6841 tctcgcactg cagacacccc gtgcatttct tggggtcgag gtacaacgac ttcatcatgg
     6901 gtcgctcctc cggttgtgaa ttgtttatga attgacgggt ttcgccgaaa caatcgtttg
     6961 caaggcccat gccatcggtt cgctgtctgg cccgcaaggc agggatgccg ggtgcatttt
     7021 gcgcctatcg ctgctgccgc gccaatctcc gatcactgcc acgtcgctgc cgggctggcg
     7081 acgggtcgac agcgcaccgc gccgtgccat cgctgccgca cgagctgcga gccgcagccg
     7141 gcgcacctgt aacgaaacgt gacagtgcgc gcggaccctg tttcagaata ttctgcgcgc
     7201 cgcgtctgtc aaatcggcag acttgggtag tccggtctgt gcgcttggcg tccaagcttg
     7261 acagcccttg gcgcttgggg gtttccttgt ccaatcgggc ggttgtccag atcgccctct
     7321 tcggcacgaa gcttgtatga agtgtccatc cgggagagga aagaaaacga catgagcaac
     7381 tcatcgagct gtgcggcacc cgaatctggg gcaacgctca cggtcgcggc tgcacagcgg
     7441 cagattctcg acacgctgca acctatttcc ggctatgaag cggtcgccgt gcgcgacgcc
     7501 ttcggccgcg tgctggcgcg cgacgtggtc gcacccttcg acgttccggc ccacgataac
     7561 tcggcaatgg acggtttcgc ggtccgcgcc gaggatcttt cggccgagcg gccgacgacg
     7621 ctgcaggtgg tcggtacggc gcttgccggc ggtggcttct cgggcatcgt tggcagtggg
     7681 caggcggttc gcgtcatgac cggggcggtc ctgccgcgcg gcgcagatgt cgtggtggtg
     7741 caggaggtcg tccgcgaggc cggcgaacgc ggagtcgaga tccccaccgg ccagcgcacc
     7801 gggcaaaaca tccgccgcgc cggcgaggac atcgctcgcg gcaatgtcgc actgccggcc
     7861 ggaaaacggg tcggggctgc cgagttggga ttgctggcct cgctgggcat tgccgaggtc
     7921 gctgtggtcc ggcgcctgcg cgttgcgttt ctgtcgaccg gcgacgaact cgcttcgatc
     7981 ggtcgctcgc tggcgccggg cgaggtctac gacagcaacc gctacacgtt gcatggcgcg
     8041 ctgcggcggc tcgatgtgga cctgatcgac atgggcgtcg tccgcgacga gccagcggcg
     8101 atggaatcgg ccttcagcct ggccgctgcg caggccgacg tggtcattac caccggcggc
     8161 gtctcggtag gcgaggccga cttcgtcaag gcgatggtcg cccgcctggg cgaggtgcgt
     8221 ttctggaaga tcgccatcaa gccgggtcgg ccgatggcct tcggccgcat cggcgatgcc
     8281 tggctgttcg gcctgccggg gaatcccgtg gcggtgatgg tggccttcta tctcttcgtc
     8341 gccgacgcgc tgcgcaagct gtcagggcag gacccgttgc ccgttcatcc gaccctgcgc
     8401 gcgccctgcg ccggcgctat ccgcaagatg aagggtcggc gcgagttcgt ccgcggcatc
     8461 ctcttcgagg gcgacggcgg ctggcaggtc aggccggggc cgctgcaggg ctcgggagtg
     8521 ctttcgtcga tgtcgtcggc caactgtttc atcgtgcttc ccgaagaagg aagcgacgtg
     8581 gcgccaggcg atctggtcga ggtgcagccc ttcgaaggcc tctggtaggg atcgcgccgt
     8641 gaacgacgaa caactgctgc gctacagccg ccacatcatg ctggcgccga tcgacgttga
     8701 cggccagcag cgcttgatcg acggtcacgc gctgatcatc ggcgccggcg gcctcggggc
     8761 gccggcggcg ctctacgtcg catcggccgg ggtcgggcga atcaccatct gcgacggcga
     8821 taccgtcgat ctcaccaacc tgcagcggca ggtcatccat cgcgaaagca caattggcgt
     8881 caacaaggcg ttgtcggcgc agcgcgcgat tgccgacatc aatcacgact gccgggtcat
     8941 tccggtaccc aagcgggtcg gcgacgacga actcgcggcg ctggtcgccg acgccgacgt
     9001 cgtgctcgac tgtagcgaca acttcgcaac gcggcacgcc gttaacgtcg cctgcgtcga
     9061 acggcgaaag ccgctggtga gcggtgcggc catccggttt tcggggcaag tggtcgtctt
     9121 cgatccgcgg cgtgacgatg cgccgtgcta cgaatgcctg ttcccggccg gtggcggcga
     9181 cgtcgaggaa cgctgcgcgg tcatgggcgt ctttgcgccg ctgaccggca tcatcggtac
     9241 actgcaggcc gccgaggcga ttcgcctgct gcttggcctc gacggcggta ccgatggcga
     9301 actggtgctc ttcgatgcgc agtcgatgga ctggcagcgc atgcgttttc gcaaggatcc
     9361 ggcgtgccgg gtctgtggca gcgggcatgc caaccaccac gaagccgtgc tgggcgcgcc
     9421 tcaggcaggc gattttcgca tctgaaatcc ggcagcggca gggcgtggcc gccccgccac
     9481 gccggagcca actccaaacc tgccgagtcg atttccccga tgcgagtcct gctgatctac
     9541 tccaaccaga gccgtgaact cgtgccggcg ccgccggtcg gcgtcagcta cgttgctagc
     9601 gccagcgagg ccgccgggca ttcgactcgg ctgctcgatc tggccttttc ccgcgatgtt
     9661 gctgccgata ttcgcaccgc catccgcgcc cttcggcccg aggtcgtcgg cctgtcgatc
     9721 cgcaacatcg acaatctggt ccagcagcgt ttcgaatccc cgcaggcagc gctgctggca
     9781 cagatcgcgg tgattcgccg cgaggccagg cgcgccgacg gaacgccggt gccgctggtc
     9841 ctcggcggcc ccgcggtctc ggtactcggt gaggcggcga ttacgatttt cgacgccgac
     9901 ttcgcggtca tcggcgaagg cgagaccagc tttccgcagc tgctatcggc actggaagcc
     9961 ggtgattcgg cagcgctggc cgcggtcgag ggactgtgct ggcgccgcga gggcgaagtc
    10021 gtcgtcaatc cggtcggccg cctcggacag ttcgccggct cgggcatgga acgctgggtc
    10081 gactgggatg cctaccagcg ccagggcggt acctggccga tccagaccaa gcgcggctgc
    10141 ccgatgcgct gcagttattg ctcctacccc ttgatcgagg gtcggcgcgg tcgcctgcgc
    10201 gagcccgctg aagtggtcga cgagatcgag cgcgtgctgc gcgaaaccag ctgcaatggc
    10261 ggggttcggc cgcgaacctt cgagttcgtc gattcgacct tcaacgttcc cgcatcgcac
    10321 gccatcgcga tctgcgagga aatcatccgc cgccgcgtcg gtaccagctt cacggctatg
    10381 ggaattaacc cgcgcgacgt gccccccgag ttgttcccga tcatgaagcg cgccggattt
    10441 aactcggtga tgatcacgcc ggaatcggcc agcccggcga tgttggagaa ttacgtcaag
    10501 ggcttcacga tggctgaggt cgagcagacc ctgcagcacg tgcgcgcggc caaactcaag
    10561 agcatgtggt tcttcatgct cggcggcccc ggcgagacca tggaaacctg cgaggaatcg
    10621 atcgccttcg cgcgcgatcg gctgcgcagc cgttactttt ttacggtctt cttcaccggc
    10681 atccgcgttc tgcccggcac gctgctcgcc cgccaggcca tcgaacgagg ccatctcgat
    10741 cccgccgccg atctgtccgg tggtgtcttc tatctgtcgc cggcggtcga cgagcagcgc
    10801 gtcatcgacc ggatcaacgc ggcgatcgtg cgcaatccag cgatcgtgca tgccgccgaa
    10861 ggcggatcac ggtcgcagcc gccgctctac cgcgcgctga acgccttgcg cgtggcgccg
    10921 ccgtactggc gttttctgcc ggagtttctc ggatttccgc cgctgcattt cctgcgcagc
    10981 cggaacccgt cgatccgcgc cgccggatag cggtcgtgac gattgccgtt cccggcaaac
    11041 ggggttagcg tgtcggcgac gcaatttccg cattgaaggg agtgctgtga agaaactaaa
    11101 acatgaaaac gagctgttca aggcggcgct gcaggccggc gtcgaatacg ccgaaggccg
    11161 cggggtcgtc gagttcgagc cgaccgattc ggccaaagag aagctgctct acatctaccg
    11221 cctgctcgtg cacgacaagg tgatctcgcc gatgcccgag gagcaggtat cagagaagac
    11281 gctgcgtcac cgcgtttcga tgtggtacgc gcaccagctg cccaaggatc acccgctttt
    11341 gcagtgattc cgggccgccg gcgcggccgg cggggtcagc gcagattcag cgcagatcga
    11401 ggcggcggaa cagatcctcg atgactcggt gccccttgtt ggtcgggtgc acctcgaagg
    11461 tattgaggaa tccctttttc tcgaagcgct cgatcaacag caggccgctc tgaccgaaga
    11521 aggcgctgta gacgtcggcc accgcgatta cgttgtcctg gcagatcgac atctgcgcaa
    11581 ccccttcggc aacctgcgcc atgacgccgt taaacagcgg cacgacctgc tcggtgccgc
    11641 cgatctcggg aatcacgtag agattgtgca ggtagatgcg cccgccctcg g
//
LOCUS       DATDAA010000231         2434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_156943,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000231 DATDAA010000000
VERSION     DATDAA010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156943"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..>2434)
                     /locus_tag="VIS73_07445"
     CDS             complement(29..>2434)
                     /locus_tag="VIS73_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY5763027.1"
                     /translation="LAAATLFRPRCEVRETDEGSSLVVVNRAGVPASPEGLLLAYDAP
                     ESLDGPSSVAGSEPRQELMAALTVSSEGSRALQSGQFDDAVSNFEQASLMYAEAGDPA
                     ARDQALRSLSQAQQASGDYEGSVATLDKLLSAAEESENEAGVAQALASLGNAYIALEQ
                     PERAREHLSRGVMVAIEAERPEIAALNLNNLANLEVTEGRYDAALGRYQESAELALDA
                     GDRLQAAKALANGARVLLRANQLDATRELLEEARTELRPVEVTHDKVYVLIHIARTYQ
                     RLSQLDGGQRADFRNAFLALEEATTLAEALQDDRALAYAHGNMAELYEVEGRYDEALV
                     LTREAIAAAERAAAYESLYRWHWQTGRVLWAAGNNYDALDAYRRAVAILQITRQETAY
                     SYLREENSFRDSVGPVYRDLVDGLLRVAEAAGDADASAGLLLEARDTVEMLKAAELRD
                     YFRDECVADLEARAVSIETVSSDAAIVYPVFFPDRLELLVSTTSGLNRYTVPVGEARL
                     NDTIREFRLALQQPASLAYLEPSQKLYQWLIAPYEDYLESTGVTTLVFVPGGELRSVP
                     MAALHDGRDFAIRKYALAVTPILALTDPQPLDREDPRLLLAGISESVAGAAPLPYVPG
                     ELNAIQDLFGGRVLLDDGFTTTSFIQGMEDRELDIVHIASHGVFTGDPDKSFLLTHDG
                     RISMNELSSYIGRTRFRETPLELLVLSACETAIGDDRAALGLAGVAINAGARSALGSL
                     WSISDPATARLIEGFYSELQDPNLTRAEALRRAQVSMLDAEGDNFRHPYYWSAFLLIS
                     NWL"
BASE COUNT          387 a          834 c          786 g          427 t
ORIGIN      
        1 catttgaagt cactgacccc ttgtctcgtt acaaccagtt gctgatcaag aggaaagccg
       61 accagtagta cggatgccgg aagttatcgc cttcggcatc gagcatgctg acctgggccc
      121 tgcgcagcgc ctccgcccgc gtcaggttcg gatcctgcaa ctcactgtag aagccctcga
      181 tcagcctggc cgtcgcaggg tcgctgatag accacaggct gcccagcgcg ctccgcgcgc
      241 cggcgttgat cgcaacgccg gccagcccca gcgccgcccg atcgtcgccg atcgccgttt
      301 cgcaggcgct gagtacgagc agctccagcg gcgtctcacg gaatcgcgtg cggccgatgt
      361 aactgctgag ctcgttcatg gagatgcggc catcgtgggt caacaggaag ctcttgtccg
      421 gatcacccgt gaacaccccg tgcgaggcga tgtgaacgat atcgagttcg cggtcttcca
      481 tgccctggat gaagctggtc gtggtgaacc cgtcgtccag cagcacccgg cccccgaaca
      541 ggtcctggat cgcgttcagt tcgcccggca cgtacggcag cggcgccgca ccagcgacac
      601 tttcgctgat accggccagc aacagtctcg ggtcctcgcg gtcgagcggc tgcggatcgg
      661 tgagcgcgag tatcggcgtc acggcaagcg cgtacttgcg gatcgcgaag tcccggccgt
      721 catgcagcgc cgccatcggc acgctacgca gttcgccgcc cggcacgaac accagcgtcg
      781 ttacgccggt cgattccagg tagtcctcgt acggcgcgat caaccactgg tacagttttt
      841 gcgagggttc gagataggcc agcgacgcag gctgctgcaa cgctaaccgg aactcgcgga
      901 tggtatcgtt cagccgcgct tcgcccaccg gcacggtgta gcgattgagt ccgctggtcg
      961 tggacaccag cagttcgagg cggtccggga agaacaccgg gtagacgatc gctgcatcgc
     1021 tcgataccgt ctcgatggac accgcgcggg cctccagatc ggcgacgcat tcatcacgga
     1081 aatagtcccg cagctcggcg gccttgagca tctcgacggt atcgcgcgcc tcgagcaaca
     1141 ggccggcgct cgcatcggca tcacccgccg cctcggcgac gcgcagcagg ccgtcgacca
     1201 ggtcccggta gaccggtccg acgctgtcgc ggaaggagtt ctcctcgcgc aggtagctgt
     1261 aagccgtctc ctggcgagtg atctgcagga tggcgaccgc acgccggtag gcgtccagcg
     1321 cgtcgtagtt gttaccggca gcccacagga ccctgccggt ctgccagtgc cagcgataca
     1381 gcgactcgta ggcagccgct ctctcggcgg cggcgatcgc ctcacgcgtg agcaccagcg
     1441 cttcgtcata gcgcccttcg acctcgtaca gctccgccat gttgccgtgc gcataggcga
     1501 gtgcgcgatc gtcctgcagg gcttccgcga gggtcgttgc ttcctcgagc gcgaggaacg
     1561 cgttacggaa gtccgcccgt tgcccgccgt cgagttgcga cagccgctgg taggtgcgcg
     1621 cgatatggat cagcacgtag accttgtcgt gcgtgacctc gaccggacgc agctcggtcc
     1681 tggcttcctc cagcagttcc cgcgtggcat cgagctggtt ggcgcgcagc agcacgcggg
     1741 caccgttggc gagtgccttg gcggcctgca gccggtcacc ggcgtccagg gcaagttcgg
     1801 cgctctcttg gtagcggccg agcgccgcgt cgtaacggcc ctccgtgact tcgaggttgg
     1861 cgaggttgtt caggttcagc gcggcgatct cgggccgctc ggcctcgatg gccaccatga
     1921 cgccccgcga caggtgctcg cgcgcacgtt ccggctgctc gagcgcgatg taggcattgc
     1981 cgaggctggc aagcgcctgc gcgacgccgg cctcgttttc ggattcctcc gccgccgaca
     2041 acagcttgtc gagtgttgcg accgagcctt cgtagtcacc actcgcctgc tgtgcctgcg
     2101 acaggctgcg caacgcctgg tcccgcgccg ccggatcacc ggcctcggcg tacatcaggc
     2161 tcgcctgctc gaagttgctg accgcgtcgt cgaactgccc gctttgcagg gcgcgggagc
     2221 cttcactcga gaccgtcaac gcggccataa gttcctggcg tggctcgctg ccggcaacgg
     2281 atgacggccc gtcaagcgac tccggtgcgt cgtacgccag cagcaggcct tccggggatg
     2341 ccgggacgcc ggcacggttg accacgacca ggctgctgcc ttcgtccgtc tcgcgcacct
     2401 cacaacgcgg ccggaacagt gtcgcagcgg ccag
//
LOCUS       DATDAA010000232         3388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_86995,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000232 DATDAA010000000
VERSION     DATDAA010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86995"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1588)
                     /gene="trkA"
                     /locus_tag="VIS73_07450"
     CDS             complement(161..1588)
                     /gene="trkA"
                     /locus_tag="VIS73_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEY5763028.1"
                     /translation="MRIIILGAGQVGANVAENLASEANDITLIDLDRNRLAYLQDRLD
                     IRTVVGNAAAPSVLRAAGADDCDVVIAATQNDQTNLVACKVAHTVFRVPTRIARLRSA
                     DFLDDPVLLNPSNFAVDYAICPEKDITEYIAKLIEFPEALQVREFAGGRLSLVAVRAV
                     EGGPLVGRPLRDLPHLLPAVDFRVAAIFRHERAVPVDGDTIVRAGDEVFCLAASEHIR
                     YVLSELRRLDRPLKRLMIAGGGNIGVRLAKQLEGKYLVKLIERDRGRADAIAQDLKDT
                     LVFQGDGTDEDLLEQEGIDEMDLFLALTNDDEDNIMAATLARRMGCRRVVALINRRAY
                     ADMMQGGAIDIAISPAQIAISSMLARFRRGDVVRVHSLRRGAAEALEIVAHGDAKTSK
                     VVGRPLSEIKLPAGVHIAALVRDLDTPEVVGMHDIVTETAMGHVEIAHHDTVIEPGDH
                     VVVFCASKKLVPKVEKLFQVGFGFL"
     gene            complement(1604..2878)
                     /locus_tag="VIS73_07455"
     CDS             complement(1604..2878)
                     /locus_tag="VIS73_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5763029.1"
                     /translation="MAQIMVVDDEVGIRQLLSEILADEGHDVWLAENAATARKLRASR
                     RPDLVLLDIWMPDTDGISLLKEWSASGALTMPVVMMSGHGTIDTAVEATRIGAADFLE
                     KPIALQKLLSTVQKSLRHVVATPASDTAAGDLRALKGMRDLVRRIEQAATRSPAILLK
                     GGNPWLAELCARVVHSPQTPWRNLGNGDPLTQEVLESLAGGVLFVGDLAQLGKLQQKN
                     VAFALERVARHRILFIAGSSRPAEQLVEAGWEENLVQRLGEVWVDLPPLAAHVDDIPE
                     LATTMLQQLVARGDVPVRRLSSAALNAMRLHRWPGDWSELQAAVRHLALAALDEVIDD
                     GDVQALVGSTAPAADATDTALSELFEMPLREARDAFEKRYFEHLLRRESGNMTRLAER
                     CGLERTHLYRKLKQLGVATGRRGEAEGPTASG"
     gene            complement(2881..>3388)
                     /locus_tag="VIS73_07460"
     CDS             complement(2881..>3388)
                     /locus_tag="VIS73_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5763030.1"
                     /translation="QVRAMKDMVDDFRDYARLPQPVLARVDLSDVVRDVLALYEGSRN
                     AVDSKLDPTPQLVLGDRTQLRQVIHNLLRNAQDALPEGGRVTVATHRDVRGAQLVISD
                     DGPGFPAEIIARVFDPYVTTKSRGTGLGLAIVKKIVDEHHGSIEVANLEPRGAQVVIT
                     LPVAEGEH"
BASE COUNT          556 a         1140 c         1122 g          570 t
ORIGIN      
        1 ccgaaggcca tgatgatgaa gccaaagatg tagagaaccg ccggccagcg ccccatattg
       61 gtaccgcccg ctcagcccgg cgccggtccg cgccgggccg cagcgcaccc cgccgggggg
      121 cagcgccggc ccgcagcgac aagcgtgggg ggcgtccgca tcacaggaag ccgaagccga
      181 cctggaacag cttctcgacc ttgggcacta gtttcttcga ggcgcagaag acgacgacgt
      241 ggtcgccggg ctcgatcacc gtgtcgtggt gggcaatctc gacgtgtccc atcgccgttt
      301 cggtgacgat gtcgtgcatg ccgaccacct ccggcgtatc gaggtcgcgc accagcgccg
      361 cgatatgcac gccggccggc agcttgatct ccgaaagcgg tcgaccgacc accttggagg
      421 tcttggcatc gccgtgggcg acgatctcga gcgcctcggc ggcaccacgg cgcaggctgt
      481 gcacgcgcac cacgtcgcca cgacggaagc gcgcgagcat cgaactgatg gcgatttgcg
      541 ccggcgagat ggcgatgtcg atggcaccgc cctgcatcat gtcggcgtag gcacggcggt
      601 tgatgagcgc cacgacccgg cggcaaccca tgcgtcgggc cagcgtcgcc gccatgatgt
      661 tgtcctcgtc gtcgttggtc agcgcgagga agaggtccat ctcgtcgatg ccttcctgct
      721 cgagcagatc ctcgtcggta ccgtcgccct ggaacaccag cgtgtccttg aggtcttgcg
      781 cgatcgcgtc ggctcggccg cgatcgcgct cgatcagctt gaccaggtac ttgccctcga
      841 gctgcttggc caagcgcacg cctatgttgc caccaccggc aatcatcagc cgcttgagcg
      901 gccggtcgag gcgtcgcagc tcgctcagca cgtaacggat gtgctcgctg gcggccaggc
      961 agaagacttc atcgccggcg cggacgatgg tgtcgccgtc gaccggaacc gcgcgttcgt
     1021 ggcggaagat cgccgccacc cgaaagtcga cggccggcag cagatgcggc aggtcgcgca
     1081 aaggccgccc gaccagcggc ccgccctcga ccgcgcgaac ggcgaccagc gacaggcgcc
     1141 cgccggcgaa ttcgcgaacc tgcagggctt cgggaaactc aatgagcttg gcgatgtact
     1201 cggtgatgtc cttttcggga cagatcgcgt aatcgacggc gaaattgctc ggattgagca
     1261 acaccgggtc atcgagaaag tcggccgagc gcagacgggc gattcgcgtc ggcacgcgga
     1321 acaccgtgtg cgcgaccttg caggcgacga gattggtctg gtcattctgc gtcgcggcga
     1381 tcaccacgtc gcagtcgtcg gcaccggctg cgcgcagtac cgagggcgcg gcggcgtttc
     1441 cgaccacggt gcggatgtcg aggcgatcct gcagataggc gagccggttg cgatcgagat
     1501 cgatcagggt gatgtcgttg gcctcggacg cgaggttttc ggcgacgttg gcgccgacct
     1561 gccccgcgcc gagaatgatg attcgcatgc cgttccctgt tgttcaaccg gaggccgttg
     1621 gcccctcggc ctccccgcga cgcccggtag caacgccgag ctgcttaagc ttgcggtaga
     1681 gatgcgtccg ttcgagcccg cagcgctccg caagccgcgt catgttaccg ctttcacgcc
     1741 gcagcagatg ttcgaagtag cgcttctcaa aggcgtcgcg ggcttcgcgc agcggcatct
     1801 cgaagagttc ggaaagcgcc gtgtcggtcg catcggccgc cggcgcagtc gagccgacca
     1861 atgcctggac gtcgccatcg tcgatcactt cgtcgagcgc cgccagcgcc agatggcgga
     1921 ccgccgcctg cagttcactc cagtcgcctg gccagcgatg caggcgcatc gcgttgagcg
     1981 ccgccgagct gagccgccgt acgggcacgt cgccacgggc gaccagctgc tgcaacatcg
     2041 tcgtcgccag ttcgggaatg tcgtcgacgt gcgccgccag cggcggcagg tcgacccaga
     2101 cctcgccgag gcgctgcacc aggttttcct cccagccggc ttcgacgagt tgctcggcgg
     2161 gccggctgct gccggcgatg aacaggatgc ggtgacgggc aacacgttcg agtgcaaagg
     2221 cgacgttctt ctgctgcagc ttgcccagtt gcgccagatc gccgacgaag agcacgccgc
     2281 cggccagcga ctcaagcacc tcctgcgtca gcgggtcacc gttgccgagg ttgcgccagg
     2341 gtgtctgcgg tgaatggacc acccgcgcac agagttcggc cagccacgga ttgccgccct
     2401 tgagcaggat cgccggtgac cgtgtcgccg cctgttcaat tcgccgcacc agatcgcgca
     2461 tgcccttgag cgcccgcagg tcgccggccg cggtatcaga cgctggtgtc gcgaccacat
     2521 gacgtagcga cttctgcacc gtcgacagca gtttctgcag cgcgatcggc ttctcgagga
     2581 aatcggcggc gccgattcgc gttgcctcga ctgcagtgtc gatggtgccg tgcccggaca
     2641 tcatcaccac cggcatcgtc agtgcccccg acgccgacca ttccttgagc agcgaaatgc
     2701 cgtcggtgtc gggcatccag atatcgagca gcacaaggtc cgggcggcgg ctggcgcgca
     2761 gcttgcgcgc cgtcgcggca ttctcggcga gccagacgtc gtgcccttcg tcggcgagaa
     2821 tctccgagag cagctggcgg atgccgactt cgtcgtcgac gaccatgatc tgagccattg
     2881 ctagtgttct ccttcggcta ccggcagggt gatgacgacc tgcgcgccgc gtggttcgag
     2941 attggcgacc tcgatcgacc cgtgatgctc gtcgacgatc ttcttcacga tcgccagccc
     3001 aagtccggta ccgcgcgact tggtggtgac gtagggatcg aagacgcggg cgatgatctc
     3061 ggccggaaag cccggcccgt catcgctgat caccagttgg gcgccgcgca catcgcggtg
     3121 ggtcgcaacg gttacccgcc cgccctcggg caacgcgtcc tgcgcgttgc gcaacaggtt
     3181 gtgaatcacc tgccgcagtt gcgtgcggtc gccgagcacg agctgcggcg tcggatcgag
     3241 cttgctgtcg acagcgttcc ggctgccctc gtagagcgca aggacatcgc gcacgacgtc
     3301 actcaggtcc acgcgcgcca gcaccggctg cggcagccta gcgtaatcgc ggaagtcgtc
     3361 gaccatgtcc ttcatcgccc gcacctgg
//
LOCUS       DATDAA010000233         2902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_367261,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000233 DATDAA010000000
VERSION     DATDAA010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367261"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VIS73_07465"
     CDS             complement(<1..323)
                     /locus_tag="VIS73_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY5763031.1"
                     /translation="MNVPDYMKSLGREARQASRAMARADTGAKNRALEAIASAIRRES
                     TALVAANAEDLAAARASGLEPALLDRLTLSEKGVAAMADGVAQIAALPDPVGEIDGLT
                     FRPSG"
     gene            461..718
                     /locus_tag="VIS73_07470"
     CDS             461..718
                     /locus_tag="VIS73_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763032.1"
                     /translation="MSERKLIIPTEIKLCATCSYWDGERKVDDEINVVIVSESCEGTC
                     LYREDPKQALTDVRKVCGCMWEDLRDPPAEGGGEAEDSKKA"
     gene            complement(728..1756)
                     /gene="holA"
                     /locus_tag="VIS73_07475"
     CDS             complement(728..1756)
                     /gene="holA"
                     /locus_tag="VIS73_07475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY5763033.1"
                     /translation="MRIDADQLDSHLARGLAPIYAVWGDELLALEAADAIRAHARSQG
                     FDEREILVALAGFDWQQVTAAGANLSLFGSRKLIDLRVPNGKPGVEGAKVLKAYAENA
                     SPDNLLLVMLPDLNWQEEKAVWLNALAEAGVGVKAVAPPLAALPKWIGQRLARQKQEA
                     DPETLRFLAERVQGNLLAARQEVLKLGLLLPEGRLPADAVREAVVDVARFDLDDLRGA
                     LLAGALGDFARTLDGLRQEGEALPLVLWAITEEIRALLVVARGIERGAPVDALLREAR
                     VFGPRQGAIRRAANRYDAASLERAMKSLAGIDRMIKGVALGDPWDSLLHMVVTLARSG
                     SGRQSRRA"
     gene            complement(1758..2294)
                     /gene="lptE"
                     /locus_tag="VIS73_07480"
     CDS             complement(1758..2294)
                     /gene="lptE"
                     /locus_tag="VIS73_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY5763034.1"
                     /translation="MQRGRQRSIRGTAHLAAAALLLAIAGCGFALRGAIHIPYDPLHL
                     AIPAYSNFGAQLKRAVIAQGSARLTEIPGEAMAILTPTGEAREKTILSLNSAGRAREF
                     QLQYRYSFRLHDHGGRDLIEPATIVLTRDMSFDDAAVLAKEQEEALLWRDMESDLVQQ
                     VLRRLAVSPQPVAGSVAR"
     gene            complement(2297..>2902)
                     /locus_tag="VIS73_07485"
     CDS             complement(2297..>2902)
                     /locus_tag="VIS73_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041224677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY5763035.1"
                     /translation="DAGVEGAHRFLKRLWKVAHTHVSGGIVAACRTSSGLDEGQKELR
                     RKLHQTIAKVADDYGRRKQFNTAVAAVMELVNALERTALADTAGRALAQEALEAIALL
                     IYPIAPHTGYALYAELRPGSDAGQQSFPQVDDSALVQDEIELVLQVNGKLRGNLRVAA
                     SAEREAIEALAKAHEQTVKFLEGRTPKKIVVVPGRLVNVVG"
BASE COUNT          472 a          933 c          998 g          499 t
ORIGIN      
        1 atgcccgagg ggcggaacgt gaggccgtcg atctcgccga ccggatcagg cagcgcggcg
       61 atctgcgcga cgccgtcggc cattgctgcg acgcctttct cggagagggt cagccggtcg
      121 agcagggccg gttcgaggcc gctggcgcgg gcggcagcga ggtcttcggc gttggcggcg
      181 accaatgccg tcgattcacg gcggatcgcc gaggcgatcg cttcgagcgc acgattcttg
      241 gcgccggtat cggcgcgtgc catcgcgcgt gatgcttggc gggcctcgcg gcccagagat
      301 ttcatgtaat cgggaacgtt cacggtctcg gtccggtcga taagcgggat gctgctaatc
      361 agcggctgat tataagcggc agaaaaatct tcgcgtaccg gaaacatttt ccagtgatcc
      421 ggctccaaag aaacaccaga gcatccaagg ttattgcccc atgagtgaac gtaagctgat
      481 catccccacc gaaatcaagc tctgtgcaac ctgcagctac tgggacggcg aacgcaaggt
      541 cgacgacgaa atcaacgtcg tcatcgtctc ggaaagttgt gaaggcacct gtctttaccg
      601 cgaagaccct aagcaggcat tgacggacgt gcgcaaggtt tgcggatgca tgtgggaaga
      661 cctgcgcgac ccgcccgccg agggtggcgg cgaggcagag gacagcaaga aggcgtagcg
      721 cccgcagtca ggcgcggcgc gactggcgtc cgctaccgga ccgcgccagc gttaccacca
      781 tatgcagcaa tgaatcccat gggtcgccga gtgcgacgcc cttgatcatc cggtcgattc
      841 cagcgagcga tttcatcgcc cgctccagcg aggccgcatc gtatcggttc gcggcgcggc
      901 gaatcgcccc ctgtcgcggc ccgaaaacgc gcgcctcccg caacaaagca tcgaccggcg
      961 cgccgcgttc gatgccgcgg gcaacgacca gtagcgcgcg gatctcttcc gtaatcgccc
     1021 acagcaccag cggcagcgcc tcgccttcct ggcgcagtcc atccagcgtg cgcgcgaagt
     1081 cgccaagcgc gccggcgagc agcgcgccgc gcaggtcgtc gaggtcgaag cgggcaacgt
     1141 caacgaccgc ctcgcgcacg gcatcggcgg gcagtcgccc ttcgggcagc agcaggccga
     1201 gtttgaggac ttcctggcgc gctgcgagca ggttgccctg aacgcgttcg gcaagaaagc
     1261 gcagcgtttc tgggtcggct tcctgtttct gtcgcgctag tcgctgaccg atccattttg
     1321 gcagtgccgc cagcggtggc gcgacggctt tgacgccgac gccagcctct gccagtgcat
     1381 tcagccagac ggccttttct tcctgccagt tgaggtcggg cagcatcacc agcagcagat
     1441 tgtcgggact ggcgttctcg gcgtaggcct tgagcacctt ggcaccttcg acaccgggct
     1501 tgccgttggg aacgcgcagg tcgatcagct tgcggctgcc gaacagcgag agattggcgc
     1561 cggccgccgt gacctgttgc cagtcgaagc cggcgagtgc gacgaggatc tcgcgttcgt
     1621 cgaagccctg gctgcgggcg tgggcgcgga tcgcatcggc ggcttcgagc gccagcaatt
     1681 cgtcgcccca gacggcgtag atcggcgcca gcccgcgggc caggtgcgaa tcgagctggt
     1741 cggcgtcgat gcgcatgtca gcgcgcgacg ctgccggcca caggttgcgg cgatacggcc
     1801 agccggcgca ggacctgctg cacgaggtcg ctctccatgt ctcgccagag caaggcttcc
     1861 tcctgttcct tggcaagcac ggcggcatcg tcgaaggaca tgtcgcgggt gaggacgatg
     1921 gtcgccggtt cgatcaggtc gcggccgccg tggtcgtgca ggcgaaagct gtagcggtac
     1981 tgcagctgga actcgcgggc gcgaccggca ctgttgagcg acagaatggt cttttcacgt
     2041 gcttcgccgg tcggcgtgag gattgccatc gcctcgccgg gaatctcggt cagccgcgcg
     2101 ctcccctgcg caatgacggc gcgcttgagc tgggcgccga agttgctgta cgccgggatc
     2161 gccagatgca gggggtcgta cgggatatgg atggcgccgc gcagggcgaa gccgcagcct
     2221 gcaatggcga gcagcagcgc cgcggcggcg aggtgcgccg tgccgcgaat cgagcgctgg
     2281 cggccgcgct gcacgatcag ccgaccacgt tcacgaggcg gcccgggacg acgacgatct
     2341 ttttcggcgt gcggccttcg aggaacttga cggtctgctc gtgggccttc gccagcgcct
     2401 cgattgcctc gcgttcggcg ctcgcggcga cgcgcaggtt gccgcgcagc ttgccattga
     2461 cctgcagcac cagttcgatc tcgtcctgca ccagcgccga atcgtcgact tgcggaaaac
     2521 tttgctggcc ggcgtcagag ccggggcgca attcggcgta gagcgcgtag ccggtgtgcg
     2581 gggcgatcgg gtagatcagc agcgcgatcg cttcgagtgc ttcctgggcc agcgcgcgtc
     2641 cggccgtgtc ggcgagcgcc gtgcgttcga gcgcattgac cagttccatg accgctgcca
     2701 ccgccgtgtt gaactgcttg cgccgaccgt agtcgtcggc gaccttggca atcgtctggt
     2761 gcagcttgcg ccgcagttcc ttctggccct cgtcgaggcc gctgctcgtg cggcaggcgg
     2821 caacgatgcc gccgctgacg tgggtgtggg cgaccttcca cagccgcttg agaaaacggt
     2881 gcgcgccttc gacgccggcg tc
//
LOCUS       DATDAA010000234         2765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_51989,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000234 DATDAA010000000
VERSION     DATDAA010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51989"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..>2765
                     /locus_tag="VIS73_07490"
     CDS             70..>2765
                     /locus_tag="VIS73_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763036.1"
                     /translation="MRVHRARLRFDIDVQRFGPSEFQDLLLDVIEIGADGQVRNATLS
                     GVNTSFMGRMPFAAESLQALRFEPGGGEPAPSARLVSMSGVASFPHFGGYRMTLAHTP
                     SGARVDDISPTQGSGVHGDPKLLHLELGALNYRNGYVDGKGQFKEFLGLSQISVLEAV
                     NLVGLASVGADQVGLYERVGLGIGVDPVRAVMLYLSGPISVGVKIGAGAINVATGGKT
                     SAVSETTQLVGSAIDLATESTPAGVRDKLLTVLNDSLALTRRIHRDVGGREDDEVGTG
                     IAVAGIALNAVTGLTKAEADSRKVALAVLDALDLSLAKVGKLKVDGRALPNDASNTLA
                     MARFAAKGVRAYVDDEQFTKAEWVEIIKQLLDAVYGFSTDPGYRLGIEVLRGLNEVAS
                     LSDRFDAVVAMRLMSRTLQVVAGSTLVTGEPRDALVLSRALIEGLILARDMPNSNRVL
                     AVAGSVLDAAVGPDTHFGGGAAAADTKKHLKMARRVVDLVGQAGAGIEYADAARMVQT
                     TLHETIDTSDKPYVTVDGLVSAWAAIDGRPLPASLDAIETFLRCDGADADCASTEEAA
                     IVVEVVNAALAATTPADLLAALHRLKRLWSQASTGGWWDEFTEAYDETRRGQVRAKAE
                     GTIAQVLQTRLAARNFREFSRHSSMALSLFTALRDGWSGDASDFEAATAEADIMGFGD
                     ATMRQIRGFIEQDIIEIQRAPTMEAMQPFALEILGIERQFALLGVQTPTDWPDGLAVV
                     MNKGGQWANQASYRLQHAQTLEEYQTNAATLLSIERQRVLLGADDDDVLVNANILDPH
                     GLVTRLIQQKINTARTSADLAAGIRELLGLERQRQLLGALDKKTMYDVIPDSLFVRYE
                     TALRGEMEATLRATGASIKEKYAEVRGRLETATRLEDADGARA"
BASE COUNT          475 a          914 c          935 g          441 t
ORIGIN      
        1 atccgcggcg gacgctcgcc gcttgccgta ccggccgtgc cgccagtgtt gacgctggat
       61 cggtcattcg tgcgcgtgca ccgcgcgcgc ctgcgcttcg acatcgacgt gcagcgcttc
      121 ggcccgagcg agttccagga cctattgctc gacgtcatcg agatcggcgc cgacgggcag
      181 gtgcgcaacg cgactctctc cggggtcaac accagcttta tgggccggat gccgttcgcg
      241 gccgagagcc tgcaggcgct gcgcttcgag cccggcggcg gcgagcctgc gccctcggcg
      301 cggctcgtct cgatgagcgg cgtcgcgagc ttcccccact tcggcggcta ccgcatgacg
      361 ctcgcgcaca cgccgagcgg cgcacgcgtc gacgacatct ccccgacgca gggcagcggc
      421 gtgcacggcg acccgaagct gctccacctc gagcttggcg cgctcaacta ccgcaacggc
      481 tacgttgatg ggaagggaca atttaaggag ttcctcggcc tgtcgcagat ctcggtgctg
      541 gaggcggtga atctcgtcgg gctggcttcg gttggcgccg atcaggtcgg cctgtacgag
      601 cgcgtcgggc tcggcatcgg cgttgatccg gtgcgcgcgg tcatgctcta cctcagcggg
      661 ccgatctcgg tcggggtcaa gatcggagcg ggcgcaatca acgtcgccac cggcggcaag
      721 accagcgcgg tttccgagac cacgcaactg gtcggctcgg ccatcgacct cgccaccgag
      781 tccaccccgg cgggcgtgcg cgacaaactg ctcaccgttc tcaacgactc gctcgcgctc
      841 acccgccgca tccaccgcga cgtcggcggg cgcgaggacg acgaggtcgg caccggcatc
      901 gctgtggcgg ggatcgcgct caacgcggtc actggcctga ccaaggccga ggccgacagc
      961 cgcaaggtcg cgctcgccgt actcgatgcg ctcgaccttt cgctggccaa ggtcggcaag
     1021 ctcaaggtcg acgggcgggc cctaccgaac gatgccagca acaccctggc catggcgcgc
     1081 ttcgccgcca agggcgtgcg cgcctacgtc gacgacgagc aattcaccaa ggcagagtgg
     1141 gtcgagatca tcaagcaact gctcgacgcg gtatacgggt tctccaccga tcccggctac
     1201 cgcctgggca tcgaggtgct gcgcggcctg aacgaggtcg ctagccttag cgaccgcttc
     1261 gacgcggtcg tggcgatgcg gctgatgagc cgcacgttgc aggtggtcgc cggatcgacg
     1321 ctggtcacag gcgaaccgcg cgatgcgctg gtcctgagcc gagcgctgat cgaggggctg
     1381 attctcgccc gcgacatgcc gaattcgaac cgtgtgctgg cggtcgcggg cagtgtgctc
     1441 gacgctgcgg tcgggccgga tacgcacttc ggcggcggcg cggcggcggc cgatacgaag
     1501 aagcatctca agatggcgcg ccgcgtggtg gacctcgtgg ggcaggccgg cgccggcatc
     1561 gagtacgccg acgcggcgcg gatggtgcag accacactgc acgagacgat cgacacgagc
     1621 gacaagccct atgtgaccgt tgatgggctg gtatccgcct gggcggcgat cgacggccgg
     1681 ccgttgcccg catcgctcga tgcgatcgaa accttcctgc gctgcgacgg cgccgatgcc
     1741 gactgcgcaa gcactgaaga ggccgcgatc gtagtcgagg tggtgaacgc tgcgctagct
     1801 gccaccacgc cggccgacct gcttgcggcg ctccatcgcc tcaaacgcct ttggtcacag
     1861 gcgtccacgg gcggttggtg ggacgagttt accgaggcat acgacgagac ccgtcgcggg
     1921 caggtgcgcg ccaaggccga gggaacgatc gcacaggtgc tgcagacgcg actcgcggca
     1981 cgcaacttcc gtgagttttc ccgccattcg tccatggcgc tgtcactgtt caccgcgttg
     2041 cgcgatggct ggtccggcga cgcgagcgac ttcgaggcgg cgaccgccga agcagacatc
     2101 atggggttcg gcgacgccac gatgcggcag atccgcggct tcatcgagca ggacatcatc
     2161 gagatccagc gcgcacccac catggaggcg atgcagccgt ttgccctgga aatcctgggg
     2221 atagagcggc agttcgccct gctcggcgtc cagacaccaa ccgactggcc agacggccta
     2281 gcggtcgtca tgaacaaggg agggcaatgg gccaatcagg cctcttaccg cctgcagcat
     2341 gcacagaccc tcgaggaata tcagacgaat gcggctacct tgctgagtat cgaacgccag
     2401 cgcgtgttgc tcggtgcgga cgacgacgat gtcctcgtca acgccaacat ccttgatccg
     2461 catggcttgg tcacgcgatt aattcagcaa aagatcaaca cggctcgcac ttcggcagat
     2521 ttagcggccg gcatccgtga actgctcggt ctcgagcgcc agcgccagct tctgggggct
     2581 ctcgataaaa aaactatgta tgacgtgata cccgattcct tgttcgtccg ctacgagacg
     2641 gcgttgcgcg gcgagatgga ggcgacgctg cgcgccaccg gcgccagcat caaggagaag
     2701 tacgcggaag tgcgtggacg cctggagacg gcgacgcgcc tcgaagatgc ggacggcgcg
     2761 cgcgc
//
LOCUS       DATDAA010000235         2089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_297787,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000235 DATDAA010000000
VERSION     DATDAA010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297787"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="VIS73_07495"
     CDS             complement(<1..1196)
                     /locus_tag="VIS73_07495"
                     /EC_number="1.13.11.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gallate dioxygenase"
                     /protein_id="HEY5763037.1"
                     /translation="MARIIAGLAASHTPTIAFAKDTKSEDDPAWADVFKVFRPLQAWL
                     EHKRPDALFFIYNDHITSFFFDHYSPFVLGVDDRYVTADEGGGPREYPPVPGHLGLAR
                     HIGSSLFADEFDISFFQKKPIDHGLFSPLSMMTDRGEAWDGAVIPFQVGVLQFPIPTA
                     RRCYRLGQALRRAIESYPEDLSVAIVATGGLSHQVHGERCGFNNTEWDHEFLDLLEND
                     PEKLTEITHAEYVERGGLESAEIIMWLIMRGALPEKIRCVHRDYCLPTMTPIATLIYE
                     SDAEPSAGEADRQRRRIAAQLGGIEQIDGTHPFTLDVSHRAFRLNDFLHRLVEPEHRR
                     RFLEDSDALYGEYGLSDEERGLLDARDWIGLIHYGVIFFSLEKMAAVIGSSNPAIYAQ
                     MRGETM"
     gene            1565..>2089
                     /locus_tag="VIS73_07500"
     CDS             1565..>2089
                     /locus_tag="VIS73_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5763038.1"
                     /translation="MALCACQREEPAATETPQAAITEEGDRVAWVDAERIRNADSEPG
                     NWLAHGRTWSEQRFSPLDQVNVSNVGELGLAWYVDLDTNRGQEATPIVVDGVMYSTSA
                     WSKVQAVDAKSGQLLWQYDPEVAGIWDVRACCGVQNRGAAVFRGRVYSGTLDGRLISL
                     DAATGELIWEINTTD"
BASE COUNT          373 a          646 c          697 g          373 t
ORIGIN      
        1 tccatggtct cgccgcgcat ctgtgcgtaa atggcgggat tcgaggaccc gatgaccgcc
       61 gccattttct ccaggctgaa gaaaatcacg ccgtagtgga tgaggccgat ccagtcccgc
      121 gcgtcgagaa ggccgcgctc ctcgtccgat agcccgtact cgccgtagag cgcatcgctg
      181 tcctccagga aacgacgccg gtgctccggc tcgacgagcc ggtgcaggaa gtcgttgagc
      241 cggaatgcgc ggtggctgac gtccagcgta aacgggtgcg tgccgtcgat ctgctcgatg
      301 ccgccgagct gcgcggcgat gcgccgcctc tggcggtccg cctcgcccgc cgacggctcg
      361 gcgtcgctct cgtagatgag cgtcgcgatg ggcgtcatcg tcggcaggca gtaatcgcga
      421 tgcacgcagc ggatcttttc cggcagcgcg ccgcgcatga tgagccacat gatgatctcg
      481 gcgctctcga ggccgcctcg ctcgacgtac tcggcgtgcg tgatttccgt gagcttttcc
      541 gggtcgttct cgagcaggtc caggaactca tggtcccact cggtgttgtt gaatccgcag
      601 cgctcgccgt gaacctggtg cgagaggccg ccggtcgcga cgatggcgac cgacaggtcc
      661 tcgggatagc tctcgatggc gcgccgaagc gcctggccca gccggtaaca gcgccttgcc
      721 gtcgggatcg ggaactgcag cacgccgacc tggaaaggga ttaccgcgcc atcccaggct
      781 tcgccgcggt cggtcatcat ggacagcggc gaaaacaggc cgtggtcaat cggtttcttc
      841 tggaaaaacg agatatcgaa ttcgtcggcg aacagcgaac tgccgatgtg ccgagcgagg
      901 ccaaggtggc cgggcaccgg cgggtattca cgcggcccgc cgccctcgtc cgccgtcacg
      961 tagcggtcgt cgacgccgag cacgaatggc gagtagtgat cgaagaagaa ggaggtgatg
     1021 tgatcgttgt agatgaagaa cagcgcgtcg gggcgcttgt gctccagcca cgcctgcagc
     1081 ggccggaaga ccttgaaaac gtcggcccag gcgggatcgt cttcggactt ggtatctttg
     1141 gcgaacgcga tggtcggtgt gtgcgaggcc gcgaggccag cgattatcct tgccatgtct
     1201 tttgctccgt ctgcaaagga agccgctcaa tcgcctgtcg ggaagtcacg gattccgcgt
     1261 cgcccccggc aatgaccggt caggcctgcg atcgcctgaa acggtcgaat gcgccttcag
     1321 tcgtcgtgat tttcagttag gccaagtcta cccggcgcgc acgggtgccg ccacaggcga
     1381 cgggcaatcc cgggctccag gccgcggcgc gacggtcctg cgacgatccg cgccccgttg
     1441 tggtaacgtt ggtttcgctt ttgagcgaca gcgaacaatc gcgcttcctg cacaagaata
     1501 accaggggag atcgttatga atgaagcatt tcgcccggca gcggtcctgc tggcgttctc
     1561 gaccatggcg ctttgcgcct gtcagcgtga ggagcccgcg gccacggaaa ctccgcaagc
     1621 agcgattact gaagaaggag atcgggtcgc ctgggtcgat gcggaacgca tccgcaatgc
     1681 cgacagcgaa ccgggaaact ggcttgccca cgggcgaaca tggtccgagc agcggttcag
     1741 cccgctcgac caggtcaatg tttcgaacgt cggcgaactc gggctggcct ggtacgtcga
     1801 tctcgacacc aatcgagggc aggaagcgac gcccatcgtc gtcgacggcg tcatgtattc
     1861 gaccagcgcc tggagcaagg tgcaggcggt ggacgcaaaa tcggggcagc tgttgtggca
     1921 gtacgatccc gaagtggccg gcatatggga cgtccgcgcc tgttgcggcg tgcagaaccg
     1981 gggcgccgcc gtcttccggg gacgcgtcta ctccggcacg ctcgacgggc ggctcatttc
     2041 gctcgacgct gcgaccggcg agctgatctg ggaaatcaac accacggac
//
LOCUS       DATDAA010000236         3381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_17276,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000236 DATDAA010000000
VERSION     DATDAA010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17276"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..316)
                     /gene="tusB"
                     /locus_tag="VIS73_07505"
     CDS             complement(20..316)
                     /gene="tusB"
                     /locus_tag="VIS73_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HEY5763039.1"
                     /translation="MLHIVNKSPHDRPTLQSVARIAQTGAVLLIEDGVYGATKGNAAA
                     GSVTELAKRVKVYALLPDLEARGVADRLIEGVTTVDYGGFVDLVAEYPTCQTWL"
     gene            complement(329..691)
                     /gene="tusC"
                     /locus_tag="VIS73_07510"
     CDS             complement(329..691)
                     /gene="tusC"
                     /locus_tag="VIS73_07510"
                     /inference="COORDINATES: protein motif:HMM:NF001238.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HEY5763040.1"
                     /translation="MSTKKFMFVNRKAPYGSIYALESLEVVLITAAFDQDVSLVFLDD
                     GVFQLKKGMQTKGIETKNFSPTYRALDGYDVEKLYVEQESLEARGLTEDDLVVDVTVL
                     SSAEMGKLMEEQDVVLSF"
     gene            complement(704..1063)
                     /gene="tusD"
                     /locus_tag="VIS73_07515"
     CDS             complement(704..1063)
                     /gene="tusD"
                     /locus_tag="VIS73_07515"
                     /inference="COORDINATES: protein motif:HMM:NF001237.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HEY5763041.1"
                     /translation="MKFGILVNEGPYQHQASDSAYLFCKAALAKGHEIARVFFYHDGV
                     NNSSKLTEPPQDDRNIVARWSKLAEEHDVDLVVCVAAALRRGIKDENLAPNFRISGLG
                     QLVETGIQADRTVTFGD"
     gene            complement(1096..2184)
                     /gene="dsrB"
                     /locus_tag="VIS73_07520"
     CDS             complement(1096..2184)
                     /gene="dsrB"
                     /locus_tag="VIS73_07520"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HEY5763042.1"
                     /translation="MSQAQAAATPRRPIECGVPDPKQYMHPSLVKNMGNWKYHDRPRP
                     GVLHHVSKTGEQVWSVRAGTQRQMDVHTIRKLADIADRFAEGHVRFTIRSNIEFIVSD
                     EKKVAPLVAELEKNGFPVGGTGNSITMIAHTQGWLHCDIPGTDASGVVKAMMDQLYEE
                     FRREEMPNRVHLSTSCCSINCGGQADIAVIVQHTKPPVINHDLVANVCERPAVVARCP
                     VAAIRPALVNGKPSLEVDEKKCVCCGACYPPCPPMQINDPEHSKLAIWVGGKNSNARG
                     KPTFMKMVASGLPNNAPRWPEVNEVVGNILRTYKEDARPWERLADWVDRIGWPRFFEK
                     TGLPFTKYLIDDWRGSRSSLNASTHVRF"
     gene            complement(2202..>3381)
                     /gene="dsrA"
                     /locus_tag="VIS73_07525"
     CDS             complement(2202..>3381)
                     /gene="dsrA"
                     /locus_tag="VIS73_07525"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     alpha"
                     /protein_id="HEY5763043.1"
                     /translation="MGQLETSYRTRKGYWKGGTVGVFGYGGGIIPRFTELKDEKGAPV
                     FPDAAEFHTLRVQPPAGMHYTTDVLRKMADIWERHGTGLIAFHGQSGDIMFQGATTAK
                     VQEAFDELNELGFDLGGAGPAVRTSMSCVGAARCEQSCYDEGRAHRQVINAFVDDIHR
                     PALPYKFKFKFSGCPNDCMNAIQRSDMAVIGTWRDNIQTDDALAKKWFAKHGLNELVN
                     DVVARCPSKALQIKPLKDLRKDAHISSVALDEAQGLEIDNKDCVRCMHCINVMTGALA
                     PGQDRGASILVGGKSHLKIGGLLGTMVVPFMKLDTDEDREKLVELAKTILDFFADNAL
                     EHERTGEMIERIGLVSFLEGVGLDVDANMVTQPRTNPYVRTDGWEDEVAKIKAKQQAA
                     "
BASE COUNT          540 a         1032 c         1120 g          689 t
ORIGIN      
        1 gggttgaaac gaaaacggcc tagagccaag tctggcaggt cgggtattcg gcaaccagat
       61 cgacgaatcc gccgtaatcg acggtggtga cgccctcgat caggcgatcg gcgacgccgc
      121 gggcttcgag atcgggcagc agcgcgtaga ccttcacccg cttcgccagc tcggtgacgc
      181 tgccagccgc ggcattgcct ttagttgcgc cgtagacgcc gtcctcgatc agcagcacgg
      241 cgccggtctg cgcgatgcgg gcaaccgatt gcagcgtcgg gcggtcgtgc ggcgacttgt
      301 tcacgatatg cagcattcca ggctcccctt aaaagctcag cacgacgtcc tgctcttcca
      361 tcagcttgcc catctcggcg ctggaaagaa cggtgacgtc tacgaccagg tcgtcttcgg
      421 tcaggccgcg agcctcgagt gattcttgct cgacataaag cttctcgacg tcatagccgt
      481 caagtgcgcg gtaggtcggc gagaagttct tggtctcgat gcccttggtc tgcatgccct
      541 tcttcaactg gaacacgcca tcgtcgagga agaccagcga gacgtcctga tcgaaggcgg
      601 cggtgatcaa cacgacttcc agcgattcga gcgcgtagat cgagccgtag ggcgccttgc
      661 gattgacgaa catgaatttc ttggtgctca tcggtccgcc tcctcaatcg ccgaaggtga
      721 ccgtacggtc ggcctggata ccggtctcga caagctggcc tagtcccgag atgcggaagt
      781 tgggggcgag gttctcgtcc ttaataccgc gacgcagggc tgcagcgaca cagacgacca
      841 gatccacgtc gtgctcttcg gcgagcttcg accagcgcgc gacgatgttg cggtcgtcct
      901 gtggtggttc ggtcagcttc gacgagttgt tcacgccgtc gtggtagaag aagacgcgag
      961 caatctcgtg ccccttggca agcgccgcct tgcagaacag gtaggcggag tcgctggcct
     1021 ggtgctggta cggcccttcg ttcaccagga tgccgaattt catcgtgttg tctccctgag
     1081 cgaatcggct tgcgttcaga aacggacgtg ggtcgacgcg ttaaggctgc tccgcgaacc
     1141 gcgccaatca tcgatcaggt atttggtgaa cggaagtccg gtcttctcga agaagcgcgg
     1201 ccagccgatg cggtcgaccc agtcggccag gcgctcccag ggacgcgcgt cttccttgta
     1261 ggtgcgcagg atattgccga ctacctcgtt aacttccggc cagcgcggcg cgttgttggg
     1321 cagacccgag gcgaccatct tcatgaaagt cggcttgccg cgggcgttgg agttcttgcc
     1381 gccgacccag atagcgagct tggagtgctc gggatcgttg atctgcatcg gtgggcaggg
     1441 cgggtagcag gcgccgcagc agacgcactt cttttcgtcg acttcgagcg aaggcttgcc
     1501 gttgaccagc gccgggcgga tcgccgcgac cgggcaacgg gcaacgacgg ccgggcgttc
     1561 gcagacgttg gcgaccaggt catggttgat gaccggcggc ttggtgtgct ggacgatcac
     1621 cgcgatgtcg gcctggccgc cgcagttgat cgagcagcag gaggtcgaca ggtgcacgcg
     1681 gttgggcatc tcttcgcggc ggaattcctc gtagagctga tccatcatcg ccttgacgac
     1741 acccgaagcg tcggtgccgg ggatgtcgca gtgcagccag ccctgcgtgt gggcgatcat
     1801 cgtgatcgag ttgccggtac cgccgaccgg gaagccgttc ttctcgagtt cggcgaccag
     1861 tggcgcgacc ttcttctcgt cggagacgat gaactcgatg ttcgagcgga tcgtgaaacg
     1921 gacgtggccc tcggcgaacc tgtcggcgat gtcggcgagc ttgcggatcg tgtggacgtc
     1981 catctgccgc tgcgtgccgg cgcgaacgct ccagacctgc tcgccggtct tcgagacgtg
     2041 atggaggacg cctggacgcg gccggtcgtg gtacttccag ttgcccatgt tcttgaccag
     2101 cgacggatgc atgtactgct tgggatccgg tacgccgcac tcgatcgggc ggcgcggcgt
     2161 ggcagcggct tgcgcttgac tcattgtctg ttctccctgt ctcaggcggc ttgttgcttg
     2221 gctttgatct tggcgacttc gtcttcccac ccgtcggtac gcacgtatgg gttggttcgc
     2281 ggctgggtca ccatgttggc gtcgacgtcg aggccgacgc cttcgaggaa ggagacgagg
     2341 ccgatgcgct cgatcatctc gccggtgcgc tcgtgctcga gtgcgttgtc ggcgaagaag
     2401 tcgaggatgg tcttggccag ctcgaccagc ttctcgcggt cttcgtcggt atcgagcttc
     2461 atgaagggga cgaccatcgt gcccagtagt ccgccgatct tcaggtggct cttgccgccg
     2521 acgaggatcg atgcgccgcg gtcctggccc ggcgccaggg caccggtcat gacgttgatg
     2581 cagtgcatgc agcgcacgca gtccttgttg tcgatttcga gtccttgtgc ttcgtcaagg
     2641 gcgaccgagc tgatgtgcgc gtccttgcgc agatccttga gcggtttgat ctgcagcgcc
     2701 ttgctcgggc agcgggcgac gacgtcgttc accagctcgt tcaggccgtg cttggcaaac
     2761 cacttcttgg ccagcgcatc gtcggtctgg atgttgtcgc gccaggtgcc gatgacggcc
     2821 atgtcggagc gctggatggc gttcatgcag tcgttcgggc agccgctgaa cttgaacttg
     2881 aacttgtacg gcagcgccgg gcggtggatg tcgtcgacga aggcgttgat gacctgccgg
     2941 tgggcgcgcc cctcgtcgta gcagctctgc tcgcagcgcg ccgcgccgac acagctcatc
     3001 gaagtgcgca ccgccgggcc ggcgccgccg aggtcgaagc cgagctcgtt gagttcgtcg
     3061 aaggcctcct gcaccttggc cgtcgtcgca ccctggaaca tgatgtcgcc cgactggccg
     3121 tggaaggcga tcagcccggt gccgtgacgc tcccagatgt cggccatctt gcgcagcacg
     3181 tcggtcgtgt agtgcatgcc ggccggcggc tgcacgcgca gcgtgtggaa ctcggcggcg
     3241 tcgggaaaca ccggcgcccc cttctcgtcc ttcagctccg tgaagcgcgg aatgatgccg
     3301 ccgccgtagc cgaacacgcc caccgtgccg cccttccagt agcccttgcg ggtgcggtag
     3361 gacgtctcca gctgacccat c
//
LOCUS       DATDAA010000237         6009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_122732,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000237 DATDAA010000000
VERSION     DATDAA010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122732"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VIS73_07530"
     CDS             complement(<1..340)
                     /locus_tag="VIS73_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY5763044.1"
                     /translation="MEFFRIRNDIPFMRHALVFNIISLVTFALAVFFLTTRGLHLSVE
                     FTGGTLVEARYSESADLDSVRGALSQAGYTDIAVQHFGSTNDVLIRLPLRTEQSSAHV
                     GDSVMAALSQA"
     gene            complement(352..2226)
                     /gene="secD"
                     /locus_tag="VIS73_07535"
     CDS             complement(352..2226)
                     /gene="secD"
                     /locus_tag="VIS73_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY5763045.1"
                     /translation="MNRYPLWKYLIVAFSLAIGFLYTLPNFFGESPAVQVSSVRSTVK
                     VDTTTLSRVEDTLGKAEIAHNGLFLDAVGVKVRFASPELQLKAKDILEREFNPDPADN
                     QYVVALNLLPASPDWLTAINALPMYLGLDLRGGVHFLLQVDMPGALTKRLDSTAADLR
                     SLMRDQKIRHAGINREGQSIEIRFRAAQELASAREAIEKSHSGELQLTERQDGDSTLL
                     VATLRPEAQKQIQNDALEQNIGTLSKRVNELGVAEPVIQRQGADRIVVQLPGVQDTAK
                     AKDILGRTATLEVRLVDEDAYRSGDTPIGGESFPERSADGTVKNVPVKRQVYVTGDRF
                     KGAQATIDQDGRPAVAVELDQAGGRIMRDVTRENLKRLMAIVLFERGKGEAISVATIQ
                     GEFGNRFQITGAFSAEETNNLALLIRAGTLAAPMEIVEERTVGPSLGADNIAKGFNST
                     LWGFVALGAFIAAYYMLFGLVSVVALSANLLLLIALLSMLQATLTLPGIAAIALTLGM
                     AIDANVLINERIREELRNGSTPQAAIAAGYERAWGTILDSNVTTLIAGVALLIFGSGP
                     VRGFAVVHCLGIMTSMFSAVIISRAMVNLIYGSRKRVEKLSIGQIWKPTANPAPEAAQ
                     "
     gene            complement(2316..2654)
                     /gene="yajC"
                     /locus_tag="VIS73_07540"
     CDS             complement(2316..2654)
                     /gene="yajC"
                     /locus_tag="VIS73_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY5763046.1"
                     /translation="MLISSAYAQAASGGGQDSLMGLLPLVLMFVVIYFLMIRPQMKRA
                     KDHKLMVEALQKGDEVVTAGGMVGRITKLADAYVTIEIAEGKDGPIEVLVQKQTVGML
                     LPKGTFKNER"
     gene            complement(2725..3837)
                     /gene="tgt"
                     /locus_tag="VIS73_07545"
     CDS             complement(2725..3837)
                     /gene="tgt"
                     /locus_tag="VIS73_07545"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY5763047.1"
                     /translation="MQFDLLASSGPARRGRLTLAHGSIETPVFMPVGTYGTVKAMAPE
                     ELVELGAEIVLGNTFHLWLRPGVEVIRAHGGLHGFMHWQRPILTDSGGFQVFSLGALR
                     KISEDGVKFASPIDGAKLFMRPEDSMQIQHALDSDVVMIFDECTPYPATHDEAAQSMR
                     LSLRWAQRSRDEHDRLGNRNALFGIVQGGMYEALRDESLAALDAIGFDGMAIGGLSVG
                     EPKEDMARILAHTAPQLPAHKPRYLMGVGTPEDLVFAVGHGIDMFDCVMPTRNARNGH
                     LFTRFGDIKIKNARFRADTTPLDPSCGCYCCRNYSRAYLHHLFRAGEILGSRLNTVHN
                     LFYYQTLMREIREAIAADGYPAYALDFRQQRQRGID"
     gene            complement(3827..4882)
                     /gene="queA"
                     /locus_tag="VIS73_07550"
     CDS             complement(3827..4882)
                     /gene="queA"
                     /locus_tag="VIS73_07550"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY5763048.1"
                     /translation="MPISQRLDDYDFELPAELIAQHPTGERSRSRLLLARRDAFADRQ
                     FAELPSLLRQGDLLVVNDTRVIKARFLGQKASGGKVEVLVERIIDGRHAIAQIRASKS
                     PGAGTTLRLEAHFDCTVTGRDGEFFTLSLGEHDPASFWDHCERCGRLPLPPYITHPAD
                     ATDESRYQTVYAREPGAVAAPTAGLHFDDALFAALAASGIGTASLTLHVGAGTFQPVR
                     VDDLTQHRMHAERFEIPPETVAAIEATRARGGRVVAVGTTSLRALEAATPEGERVPQP
                     GAGETRLFILPGYRFRCVDALITNFHLPRSTLLMLVSAFAGTATIRDAYAHAVRERYR
                     FFSYGDAMLLERNDDAI"
     gene            4984..5070
                     /locus_tag="VIS73_07555"
     tRNA            4984..5070
                     /locus_tag="VIS73_07555"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5018..5020,aa:Leu,seq:caa)
     gene            5252..>6009
                     /locus_tag="VIS73_07560"
     CDS             5252..>6009
                     /locus_tag="VIS73_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY5763049.1"
                     /translation="MRFRMNSIGTKLTAVIGVTIATGFATVVYFYTQQHERNILLQNE
                     RAVHQIVDSVGEGLNSVMITGSADVAELYADRLKDVKDVEDFRILRPDGMEAFRDNKT
                     INAVNERRGEIDFEPRQSETEQRVLATDDPHLRETVQSQNFTFYYGNKGERDVLTFLL
                     PIKSAQKCERCHGKEDVVLGLLEFTTSLDSAKAAVKQTRVMAVGVLLAALVLIIVLTR
                     YVLRRYIVTPIERVSEAMAKVSTGDLYQEVPVFGS"
BASE COUNT         1075 a         1826 c         2033 g         1075 t
ORIGIN      
        1 tggcctgcga cagcgccgcc atcaccgaat cgccgacgtg ggccgaactc tgttcggtgc
       61 gcagcggcag gcggatcagc acgtcgttgg tggaaccgaa atgctgaacg gcaatgtcgg
      121 tgtacccagc ctgcgacagc gcgccgcgaa cggaatcgag atcggccgac tcgctgtagc
      181 gggcttcgac cagcgtgccg ccagtgaatt cgaccgaaag gtgcagcccg cgcgtggtga
      241 ggaagaacac ggcaagggcg aaggtgacca gcgagatgat gttgaacacc agcgcatggc
      301 gcatgaacgg gatgtcgttc cggatgcgga agaattccat gattgcctac ctcactgtgc
      361 cgcttcggga gcgggattgg cggtcggttt ccagatctga ccgatcgaga gtttctcgac
      421 gcgcttgcgg ctgccgtaga tcagattgac cattgcgcgc gaaatgatga ccgcgctgaa
      481 catcgaggtc atgatgccga ggcagtgcac gaccgcgaag ccgcgcaccg ggccggagcc
      541 gaagatcagc agcgcgacgc cggcgatcag cgtcgtgacg ttggagtcga gaatcgttcc
      601 ccaggcgcgt tcgtagccgg cggcgatggc cgcctgcggc gtcgagccgt tgcgcagttc
      661 ctcgcggatc cgttcgttga tcagcacatt ggcgtcgatc gccatgccca acgtcagcgc
      721 gatggcggcg ataccgggca gtgtcagcgt cgcctgcagc atcgacagca gcgcaatcag
      781 cagcagcagg ttggccgaca gcgcgacaac cgataccagg ccgaacaaca tgtagtacgc
      841 ggcaatgaag gcgcccagcg cgacgaatcc ccagagcgtc gaattaaaac ccttggcgat
      901 gttgtcggcg ccaagcgagg ggccgacggt gcgctcctcg acgatttcca tcggcgcggc
      961 gagcgtgccg gcgcggatca gcagcgcgag attgttggtc tcttcggccg agaaggcgcc
     1021 ggtgatctgg aagcgattgc cgaactcgcc ttgaatcgtg gccacggaga tcgcctcgcc
     1081 cttgccgcgc tcgaaaagca cgattgccat caggcgcttg aggttctcgc gcgtcacgtc
     1141 gcgcatgatt cgaccgccgg cctgatccag ttctaccgcc acggccgggc ggccgtcctg
     1201 atcgatcgtc gcctgcgcgc ctttgaagcg atcgccggtg acatagacct ggcgcttcac
     1261 cgggacattc ttgacggtgc cgtcggcgct tcgctcgggg aaggattcgc cgccaatcgg
     1321 cgtatcgccg ctgcggtacg catcctcgtc gaccagacgc acttcgagcg ttgccgttcg
     1381 gccgaggatg tccttcgcct tggccgtgtc ctgaacgccg ggcagctgga cgacgatgcg
     1441 gtcggcgccc tggcgctgaa ttaccggctc ggcgacaccc aattcgttga cgcgcttgga
     1501 gagcgtgcca atgttctgct cgagtgcgtc gttctgaatc tgtttctgcg cctcggggcg
     1561 cagggtcgcc accaggagcg ttgaatcgcc gtcctgccgc tcggtcagtt gcagttcgcc
     1621 agagtggctt ttctcgatgg cttcccgcgc actggccaat tcctgcgcag cgcggaagcg
     1681 aatttcgatg ctctggcctt cgcggttgat gccggcgtgc cggatcttct ggtcgcgcat
     1741 cagcgagcgc aggtcggcgg cggtcgagtc gaggcgcttg gtcagcgccc ccggcatgtc
     1801 cacctgcaac aggaagtgca cgccgccgcg caggtcgagg ccgaggtaca tcggcagcgc
     1861 gttgatcgcg gtcagccagt cgggcgaggc cggtagcaga ttcagcgcaa cgacgtactg
     1921 attgtccgcc ggatcaggat tgaattcgcg ctcgaggata tccttggcct tgagctgcag
     1981 ttcggggctg gcaaagcgga ccttgacgcc gacggcatcg aggaacagcc cgttatgggc
     2041 aatctcggcc tttcccagcg tgtcctcgac gcgcgacagc gtcgtcgtat cgaccttgac
     2101 cgtggagcgg acgctggaca cctgcacggc cggcgattcg ccgaagaagt tgggcagggt
     2161 gtagaggaag ccgattgcca gcgagaacgc gacgatcagg tatttccaga gcggatatcg
     2221 attcatggcg gagtttccaa tgccctcgct ggcggatcac tccgcgcagc gacggcagaa
     2281 gcgacgcgcg aacggcgtcg ggggcgagcc agtgttcagc gttcgttctt gaacgtacct
     2341 ttgggcagca gcatgccgac ggtctgtttc tggacgagaa cctcgatcgg gccgtccttg
     2401 ccttcggcga tctcaatcgt cacgtaggcg tcggccagct tggtgatgcg gccgaccatg
     2461 ccgccggcgg tgacgacttc gtcgcccttc tgcagcgctt cgaccatcag cttgtgatcc
     2521 ttggcgcgct tcatctgcgg ccggatcatc aggaaataaa tgaccacgaa catcagaacc
     2581 agcggcagca ggcccatcag gctgtcttgg cccccgcccg aagcggcttg agcgtatgcg
     2641 cttgaaatca gcacttggtg tctccctagg taatcggaaa aatgaagccg cgcattctat
     2701 caggcccgac cggccgctcg gggctcagtc gatgccgcgc tgccgctgct gccggaagtc
     2761 cagcgcgtag gccgggtagc cgtcggcggc gatcgcctcg cggatttcgc gcatgagcgt
     2821 ctggtaatag aagaggttat gcacggtgtt cagccggctg ccgaggatct cgccggcgcg
     2881 gaacaggtgg tgcaggtagg cgcgcgagta gttgcggcag cagtagcaac cgcaactcgg
     2941 gtcgagcggc gtcgtatcgg cacgaaagcg agcgttcttg atcttgatgt cgccgaatcg
     3001 cgtaaataag tggccgtttc gcgcatttcg ggtcggcatc acgcagtcga acatgtcgat
     3061 tccgtggcca accgcgaaaa ccaggtcttc gggggtgccg acgcccatca ggtagcgagg
     3121 cttgtgcgcg ggcagctgcg gcgcggtatg ggcaaggatc cgcgccatgt cctctttcgg
     3181 ttcgccgacc gagaggccgc cgatcgccat gccgtcgaag ccgatcgcat cgagcgctgc
     3241 cagcgattcg tcgcgcagcg cttcgtacat cccgccctgg acgatgccga acagggcgtt
     3301 gcggttgccc agccggtcgt gttcgtcgcg cgagcgctgc gcccagcgca gcgacaggcg
     3361 catggattgg gcggcttcgt catgcgtagc cgggtagggc gtgcactcgt cgaaaatcat
     3421 caccacgtcg gaatcaagcg cgtgctggat ctgcatcgag tcctcggggc gcatgaacag
     3481 cttggcgccg tcgatcgggc tggcgaactt cacgccgtcc tcgctgatct tgcgcagggc
     3541 cccgagcgag aagacctgga agccgccgga atcggtgagg atcggccgct gccagtgcat
     3601 gaatccatgc aggccgccgt gggcacggat gacctcgacg cccgggcgca gccagaggtg
     3661 gaaggtgttg ccgaggacga tttcggcgcc cagttccacc agttcttcgg gcgccatcgc
     3721 cttgaccgtg ccgtaggtgc cgaccggcat gaacaccggc gtttcgatgc tgccgtgggc
     3781 gagtgtcagc cgcccgcggc gggcgggtcc gctgctggcg agaaggtcaa attgcatcgt
     3841 cgtttctttc gagcagcatg gcgtcaccgt agctgaagaa gcggtagcgc tcgcgcaccg
     3901 catgggcgta ggcgtcgcga atcgtcgccg ttccggcgaa ggcagagacc agcatcagca
     3961 gcgtcgatcg cggcaggtgg aaattggtga tcagcgcatc gacgcagcgg aagcggtagc
     4021 cgggcaggat gaacaggcgc gtttcgccgg cgccgggttg cgggacacgt tcgccttcgg
     4081 gcgttgccgc ctcgagggcg cgcagcgaag tggttccgac cgcgaccacg cggccgccgc
     4141 gtgcgcgcgt cgcttcgatg gcggcgacgg tctccggtgg aatctcgaag cgttcggcgt
     4201 gcatgcggtg ttgcgtcagg tcatcgaccc gaaccggctg gaaggtgccg gcgccgacat
     4261 gcaaggtcag gctggccgta ccgatgccgg acgcggccag ggccgcgaac agcgcatcat
     4321 cgaagtgcag gcccgccgtg ggtgccgcga cggcacccgg ttcgcgggca tagacggtct
     4381 ggtaacggct ttcgtcggtg gcgtcggccg gatgggtgat atagggcggc aatggcaggc
     4441 ggccacagcg ctcgcaatgg tcccagaagc ttgcagggtc gtgctcgccc agcgacagcg
     4501 tgaagaattc gccgtcacgg ccggttacgg tgcagtcgaa atgcgcttcc agacgcaggg
     4561 tggtgccagc acccggtgac ttcgaggcac ggatctgggc gatcgcgtgc cggccgtcga
     4621 tgatccgctc gaccagcact tcgactttgc cgccactggc cttttggccg aggaatcggg
     4681 ccttgatgac gcgcgtgtcg ttgaccacca gcaggtcgcc ttggcgcagc aggctcggca
     4741 gttcggcgaa ctggcgatcg gcgaaggcat cgcgccgggc gaggagcagg cgactgcggc
     4801 tgcgctcgcc ggtcgggtgc tgggcgatca gttccgcggg tagttcgaaa tcgtagtcgt
     4861 cgaggcgttg tgaaatcggc attggcgcgg cggcgggggg gtgttcccga acgcgtacgg
     4921 tcgagttgag cgctacgggt agttcatgga taatcccgcc acccaagcga taagcgaagg
     4981 taagccggga tggcggaatc ggtagacgca gcggactcaa aatccgccgg tggtaacact
     5041 gtgagggttc gagtccctct cccggcacca gtcgagtctg cgtcgcgccg cattgtatcc
     5101 ggcgtgatgt gcgaatgccg aagtacagat ctttgcagta gcgacagagc ggcgcatcag
     5161 acgtccgcct tcacgctggc aacccggtgg cagtccgacc ggccgattgc cggtaccgcc
     5221 caccgcagcg aattcatcaa ctgaagaacc aatgcgcttt cgtatgaatt cgattggaac
     5281 caagctaacc gcagtcatcg gcgtcacgat cgcaacggga ttcgcgacgg tcgtctattt
     5341 ctatactcag cagcacgaac gcaacatcct gttgcagaac gagcgggccg tgcaccagat
     5401 cgtcgacagc gtcggcgaag gcctgaattc ggtgatgatt accgggtcgg ccgacgtcgc
     5461 cgagctgtac gccgaccgcc tcaaggacgt caaggatgtt gaggacttcc gcatcctgcg
     5521 cccggacggg atggaagcct tccgcgacaa caagacgatc aacgccgtca acgagcggcg
     5581 cggcgagatt gacttcgagc cgcggcagag cgaaacggag cagcgcgtac ttgcgaccga
     5641 cgacccgcat ctgcgcgaaa ccgtgcagtc gcagaacttc accttttact acggcaacaa
     5701 gggcgagcgc gatgtgctga ccttcctgct gccgatcaag agcgcgcaga aatgcgagcg
     5761 ctgccacggc aaggaagacg tcgttctcgg tctgctcgag ttcaccacct cgctcgacag
     5821 tgccaaggcg gcggtgaagc agacgcgggt gatggccgtc ggtgtcctac ttgcagcgct
     5881 ggtattgatc atcgtcctga cgcgctacgt cctgcgccgc tacatcgtga cgccgatcga
     5941 acgggtttcc gaggcgatgg ccaaggtgtc gaccggcgac ctttaccagg aagtgccggt
     6001 gttcggctc
//
LOCUS       DATDAA010000238         2509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_87356,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000238 DATDAA010000000
VERSION     DATDAA010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87356"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2421)
                     /locus_tag="VIS73_07565"
     CDS             complement(<1..2421)
                     /locus_tag="VIS73_07565"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763050.1"
                     /translation="MSDTRYVKLPHLTVVAASAVLSMVVSAPAFAESAYEAPKGIVDC
                     LLPGSIRRVGGNIYQMPGRPIRTIASECEIRGGDYLLFDRANYDTSLNHWIRQAEMDD
                     VDAMLYVGEIYEKGLGREPDFAAAATWYQRATDAGNATAMISLAHLYKTGQGVERDLA
                     RAQSLYSQAFGADIPIPLDPTSVKGADQRIQTLVAEVDEVRRQKIAVELELQAARDQL
                     TTARAALDDALAGNGGNADSIRRLQADLNDQQSQIRDYQSRIVVLQAQSDELSMLRQQ
                     LADEKVEAAKLQERLSETRAEVEFGRSQLAAQRQALQDKETRFQDLLANLRTEQDRDR
                     LKASSNELDAQRQRVSELEASLDEAEEQKALYQALANDAASNEDRVATLTARISILEQ
                     QSHSRESEFEALQRAHAETQAQLDAQIAAAAAADQSSDAEIAARNAEIEHLRAVVARA
                     EQETGRHQGDVDRLAAQSAELEALRATLEREQAQSNRLQLLLTESQDQFVTANRRLAE
                     VTSARARLEEEVSSLGERLAAGDRSVEAVLDQREAELLDARQELDALRAQIASSEEEF
                     KRYEVQVSDTAARQRGAIKELREAVAQSRTEREQLEDKLSAANTQFADAKADLELQRK
                     RYTELQDELLQARAASNADEAALFEKQDELDAERRRVDRLTAEIERLTRQSQRYESEI
                     DELKQLAQVQLVEFAGPSIVMLEPDDASLVATHQLTRSAEPTRGISVIAATSASETRI
                     IRGRVDAPAGLAELTIDGWQVPFDEHNAFTQTLKLDAQSKFIRIEAVDHNGKRDTKEF
                     EYRVGDVAS"
BASE COUNT          400 a          818 c          727 g          564 t
ORIGIN      
        1 cgacgcaaca tcgccgacac gatattcaaa ttccttggtg tcacgcttgc cgttatggtc
       61 tacagcttcg atgcgaatga atttcgattg ggcgtccagt ttcagggttt gcgtgaatgc
      121 attatgctcg tcgaacggca cctgccagcc gtcgatcgtc aactctgcga ggcccgcagg
      181 tgcgtcgacc cggcctcgga tgatccgcgt ctcgctcgca ctggtagctg caatcaccga
      241 aatcccgcgt gtcggctctg ccgaccgcgt cagttgatgg gtcgctacca acgacgcatc
      301 gtcgggttcg agcatgacga tgctggggcc ggcaaactcg accagctgga cttgtgcaag
      361 ctgtttcagt tcatcgatct ccgactcgta gcgctgggac tgccgggtta gccgctcgat
      421 ttcggctgtc agccgatcga cgcgccgccg ctcggcatca agctcgtcct gtttctcgaa
      481 caaagccgct tcgtcagcat tgctcgccgc cctggcctgc agaagctcat cttgcagttc
      541 ggtgtaccgc ttgcgctgca actccaggtc ggccttggca tcggcaaact gagtattggc
      601 cgcagagagc ttgtcttcga gttgctcgcg ttctgtacgg ctttgtgcca ccgcctcgcg
      661 gagttccttg atggccccac gctggcgcgc cgccgtgtcc gaaacctgca cctcgtaccg
      721 tttgaactcc tcttcagaac tcgcgatttg cgcgcgcagc gcgtccagtt cctggcgcgc
      781 atcgagcagc tctgcctcac gctgatcgag gactgcctcg acggagcgat cgcctgccgc
      841 gagacgttcc ccgagagaag agacttcctc ctcgagccgc gccctcgcgc ttgtcacttc
      901 tgcaagacgc cggttcgccg tgacgaactg atcctgcgat tcggtcagca gcagctgcaa
      961 gcgattcgac tgagcctgtt cgcgttccag cgttgcgcgc aacgcttcga gctctgcact
     1021 ttgcgcggca agccggtcga catcgccctg gtgccgtccc gtctcctgct cggcgcgcgc
     1081 cacgaccgcg cgcaagtgct cgatctccgc gttgcgtgct gcgatttcag cgtcggacga
     1141 ctggtctgca gccgccgcgg ccgcgatctg tgcgtcgagc tgtgcctgcg tctcggcatg
     1201 cgctcgctgc agcgcctcga attccgattc gcggctatgt gactgttgtt ccagtatgct
     1261 tatgcgcgcc gtcaatgtgg cgacccgatc ctcgttcgac gccgcatcgt tcgcaagtgc
     1321 ctggtatagc gctttctgct cctccgcctc gtccagtgat gcctcaagtt cactcacgcg
     1381 ctgacgctgc gcgtccagtt cattcgagct tgccttcaac ctgtcgcggt cctgttcggt
     1441 acgcaggttg gcaagcagat cctgaaaccg ggtctccttg tcctgcagcg cctggcgctg
     1501 tgcggccagc tgcgaccggc cgaactcgac ttcagcgcgg gtttcggaca gtcgttcctg
     1561 cagtttcgct gcctcgactt tctcatcggc gagttgctgg cggagcatgc ttagctcgtc
     1621 gctttgcgcc tgcagcacga cgatacgaga ctggtaatcg cggatctgcg actgctggtc
     1681 gttcaggtcc gcctgaagtc tacgaatgct atccgcgttg ccgccgtttc ccgccagtgc
     1741 atcatcgagt gccgcccgcg cggttgtcag ttgatcgcgg gctgcctgca gctccagctc
     1801 tacagcaatt ttttgccggc gcacctcgtc gacctcggca accagtgtct gtatgcgttg
     1861 atcagcgccc ttgaccgacg tcggatcgag cggaatcgga atgtcggcac cgaaggcttg
     1921 cgaatacagg gactgcgccc tggccaggtc ccgctcgaca ccctgaccgg tcttgtacag
     1981 gtgagcgaga ctgatcatcg cagtggcgtt gccggcatcc gtggccctct ggtaccaggt
     2041 cgcggcggcc gcgaagtcgg gttcccggcc caggcccttt tcgtagattt cgccgacgta
     2101 caacatcgcg tcgacgtcat ccatctcggc ctggcggatc cagtgattga gagatgtgtc
     2161 gtagttcgct cgatcgaaca acaggtagtc cccgccgcgg atctcgcact cggaggcgat
     2221 ggtacgaatc gggcgaccgg gcatctggta gatgttcccg cccacgcggc gaatcgatcc
     2281 cggcaggaga caatcaacga tgcccttcgg ggcctcgtag gcgctttcgg cgaatgccgg
     2341 cgcgctcact accatggaca gcacagccga tgctgccacc acagtcaaat gcggtaactt
     2401 gacgtatctc gtatcgctca tggcacccaa cctctgaatg attatcattc tcgtttgagt
     2461 ctagtccaaa tcccggcctt tagcgagcgg ttccgtagcc gaatgtcac
//
LOCUS       DATDAA010000239        18902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_87616,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000239 DATDAA010000000
VERSION     DATDAA010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 18902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18902
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87616"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="VIS73_07570"
     CDS             complement(<1..1338)
                     /locus_tag="VIS73_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY5763051.1"
                     /translation="MSLNDSLRYRFLMPLLLGGVVVSLIGSVYAYRTTLAEADRALLE
                     EGVAIASALNHAAMVAHNASEEQHVVEEVYKDAGKLSLALLVSDGKVSAATIPEWIGL
                     KVSSLPDPELVQGLNASASTGGEMLRSDVSADGRYRQIVMPLEASLSDHDHEHSTSVI
                     DHLGARAAPAPGKVHIHADGKRHDHGSPAVAVADPPPPAVAGHYGERPGFLARDTHKA
                     RGQILILLDRKPVEELVRANTLRIASGMSAAVAVTMLIAFLLMTRRVLTPLAAIGEAM
                     RRRQTGDKQSRAEIRGNDELARLGATLNGMLETLGRQELDMNRLSQAVEQSPVSVVIT
                     NLEGVIEYANPRFCESTGYTRDELIGETPARIKSGLTPSQTYNEMWETIRAGKRWQGE
                     LHNRNKAGDLYWENVTISPIFDEGGRIIQYLGIKEDISVRKEYEEKLVRQANFD"
     gene            1509..1985
                     /locus_tag="VIS73_07575"
     CDS             1509..1985
                     /locus_tag="VIS73_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5763052.1"
                     /translation="MNGKERLAMRGALLMVAAVLLAACGERGDADGPSWEELAHGEAI
                     YRAECAQCHGANLEGQPDWRVRKADGKLPAPPHDASGHTWHHSREQLFAIVKFGMVPP
                     NAPEGYVSDMPAYGAKLSPAQIHAVLAYIESRWPPEVITQRKERFAQATAENATKK"
     gene            complement(1983..2302)
                     /gene="ssrA"
                     /locus_tag="VIS73_07580"
     tmRNA           complement(1983..2302)
                     /gene="ssrA"
                     /locus_tag="VIS73_07580"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01850"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="betaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01850"
     gene            complement(2311..3210)
                     /locus_tag="VIS73_07585"
     CDS             complement(2311..3210)
                     /locus_tag="VIS73_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5763053.1"
                     /translation="MAPDFPIRYVEPVFRPPSEAESLILPLTDGCSWNHCTFCEMYTA
                     PQKRFRARSADEVLTTIRACGERYGDQVRRVFLADGDALALPPRRLLETLEAIATHLP
                     AVRRISSYCLPRNLRKKSDADLKALAEAGLKLVYVGAESGNDTVLERVSKGETRESTR
                     EALDRLGAAGITRSVMILNGLGGRALSDAHADDSAALINATQPEYLATLVVSFPQGDQ
                     RLRDAFPEWEPLDTRGLIAEMRRFIAALELTRTVFRSDHASNWLILKGTLGTDKARLL
                     ARIDEALASPETAGLRPAWARGL"
     gene            complement(3263..6514)
                     /locus_tag="VIS73_07590"
     CDS             complement(3263..6514)
                     /locus_tag="VIS73_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5763054.1"
                     /translation="MNSSVHPFPRSAAGNPSAGLHRLEALVEAIDGIVWEADPATFRF
                     TFVSGHAERLLGYPASDWLSLGFWADHIHPDDRDTAVEFCTEKSRGGVDHVFEYRMLA
                     ADGQTVWLRHSVSVEVRNGIPSALRGIMVDITAQKQAQQAIAEQFHFIWQLADAIPCP
                     IFWKDGDGRYGGCNRAFEDYIGIARGTLIGKTVYDIAPPDLARLYEEADRKLLGSGGT
                     QVYEAQVQYADDSRHDVVFYKAIFTREAGAKGGLVGVMLDVTDQKRLDRELRQKSELL
                     DTVVDTMPSGISIIDRNLDVILCNERARRVMGLPDSFFAAGPVPLETLFRYNAERGEY
                     GPGEADALVAERVRRTTLNEAHHFDRVRPDGTVIEVRGEPLPGGGFVTIYSDVTEARQ
                     ARADLARESALLRAVVEHMPQGISVFDEHLVLRHWNQGFIDVLELPADAVREGVHFDD
                     LIRIPAARGEYGPGDPEQHVARIHALAMRFQPHQFERVRPNGKTHLVCGKPLLIDGET
                     KGFITTYTDISDRQKTEEALRLSSTVFERSAEGIMLTDASTRILRVNAAFTRITGYRE
                     VDVLGRTPTLLNSGTQDKTFYAAMWRTLRERGHWSGEVINRKACGGLYTEHLSVTRID
                     DADGNASGYIGIFTDVTEAREAERKIEHLSYFDALTGLPNRALLMVRIDAAIARAKQE
                     SSRFALLTIDIDRLSHINDTLGHQYGDKVLLATAQRLSAATSESEIVSRHAGDGFAII
                     VDDIEDSTATRHRVEKLLETIQAPMQLDPHPLHVEAHIGIALFPGDGHDGATLLKHAD
                     AALHHVRNEGVERYEFFRSEMNAAAMERLLLESRLRQAIEREEFVLHFQPQIDLARGR
                     VVGLEALVRWSHPEMGMVPPGKFIPIAETTGLIVELGCWVMRSACLAAKALADAGFPQ
                     LRMAVNVSARQFNQDDFADRVRRALRLADLPAASLELELTESLILQRPEHVIRTMKAL
                     KETGITFSIDDFGTGYSSLSQLKRFPIDKLKIDQSFIRDIHIDDDDAAITAAIIALGR
                     SLRLEVVAEGVETDDQRAFLVEGGCHALQGYLISKPLPFDELRRFLASQKTNG"
     gene            complement(6597..7250)
                     /locus_tag="VIS73_07595"
     CDS             complement(6597..7250)
                     /locus_tag="VIS73_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY5763055.1"
                     /translation="MTAGVFFDLDGTLADTAPDLGNALNRLRAAHNLPALPLAELRPH
                     TSSGVRGMLRAGFDIGPTAPEYESLRAHYLDLYEAALCEQTVLFPGMTDVLEALEARH
                     IPWGVVTNKTSRFARPLMQRMRLIQRLACLVCGDTTPEAKPSPLPLLHAAELAGVSPR
                     DCCYVGDDKRDIDAGRAAGMRTIAVRWGYLGVDDPIEAWNADDLIDSPTELKLLLSL"
     gene            complement(7255..7992)
                     /gene="ubiG"
                     /locus_tag="VIS73_07600"
     CDS             complement(7255..7992)
                     /gene="ubiG"
                     /locus_tag="VIS73_07600"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEY5763056.1"
                     /translation="MTTDTKPFNANADLRELEKFAELAHRWWDPNSEFKPLHDINPLR
                     AEFIRESVEQAGTKLDQSTLLDVGCGGGLLSEAMAAMGAQVTGIDLGEKALGVARLHA
                     LESGSPAKYRLVAAEALADEAPGSFDVVTCLEMLEHVPDPAQTVAACARLVRPGGHLF
                     FSTLNRNPKSYLFAIIGAEYLLRMLPKGTHDYQKFIRPAELTRWCRDSGLDVAELIGM
                     SYNPLTQTYRLGRDLSVNYLLRARRPA"
     gene            complement(8040..9362)
                     /locus_tag="VIS73_07605"
     CDS             complement(8040..9362)
                     /locus_tag="VIS73_07605"
                     /inference="COORDINATES: protein motif:HMM:NF006549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HEY5763057.1"
                     /translation="MTESVDLLIAARWIIPIEPEGVVLERHSVAVRAGRIVALLPTAD
                     AMSRFTADETIDLPDQVLLPGLVNLHTHAAMSLLRGIADDVPLMTWLSEHIWPAEGRF
                     VSEAFVRDGTLLACAEMLRGGITCCNDMYFFPGAAIEAAGTVGMRFVAGIATLEFPTP
                     YASDADDYLRKGLAVRDACRELPEVHFCLAPHAPYTVGDETFTRVVTLAEQLDLPVHI
                     HVHETASEISDSIAQHGERPLARLARLNLLGPSTIAVHAVHLDASDVDLLAAHNVSVA
                     HCPSSNLKLASGIAPITHLAARGINIGLGTDGAASNNRLDLLGEMRLAALLAKGVSGD
                     ATAIDAHAALRMATINGARALGLDSLIGSLVAGKAADLCAIRLADPETLPCYQVASHL
                     AYVAGREQVSDVWVAGRRRVASGRLLAVDDGVLARRAQSWGERIAAAH"
     gene            complement(9439..10146)
                     /locus_tag="VIS73_07610"
     CDS             complement(9439..10146)
                     /locus_tag="VIS73_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY5763058.1"
                     /translation="MKKINLYLASCSLAIVTTQAIAHGYVVDSNGTVVRNSFNECWRT
                     GFWTPADAIPECEPGMKKPEPPRAMTPPPPPPPKPAPVAAPAPAPVVVAPPPPPPPPP
                     PPPKPKRIELSGSAQFAIGSSRLTDVGRADIDRDVLEKLKGFSRIDRIEIVGHTDPLG
                     SASGNERLSRNRAAAVKEYLVSRGVDAKVVTTRGVGSAEPPEGVSCAKDQSRAKLVAC
                     YAAFRRITIDVDGVAKP"
     gene            complement(10228..10791)
                     /locus_tag="VIS73_07615"
     CDS             complement(10228..10791)
                     /locus_tag="VIS73_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY5763059.1"
                     /translation="MKQENKFRQAAIVALILSVGACATPEKKEEAPAPAPRAMPAPEP
                     VKPVEAPKPVEAPKPKKVSLKSKSMFEFDKAVLTADGKAEIDANILSKLGTLNSTDFV
                     SVTGHACRMGSHQYNQQLSEARADAVKAYLVMKGVNADIIETLGAGKTQPVPGVSCAD
                     SLPRERMIECLAPNRRVEVELKGMAPG"
     gene            11030..12097
                     /locus_tag="VIS73_07620"
     CDS             11030..12097
                     /locus_tag="VIS73_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763060.1"
                     /translation="MPDPTLPSCSRECRDSTSALEFVSCSGIADCGKCLNDSHFLGGA
                     FFGAPLAAATKALPVVHVPVPVLAGDPSEGECWLAAGPAEAGDDPATGLRFRHDGTFV
                     FGVVRLREADFAGGDGSPLQQATAAAYGAVFATMDRLGYPHLVRCWNYLADINGELDG
                     LERYRHFNIGRQDAFLAAARDTVGSVPAACALGTRDSDLLICFLAARSAALPIENPRQ
                     VSAFRYPPDYGPRSPTFARAALLPLPGRPLLLVSGTASIVGHRTVHSGDLCAQLRESM
                     ANIAAVLAEATRQAAGAEFRLGGLDYRIYLRHADDLASAKQAFGAHFAGMPKPHAVFM
                     QADVCRHDLLVEIEASGYAGP"
     gene            12097..12942
                     /locus_tag="VIS73_07625"
     CDS             12097..12942
                     /locus_tag="VIS73_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEY5763061.1"
                     /translation="MPIDRPLRIGTLRVAFALTLLGMVSPLAAEQLPLWEAGAGVGVL
                     SLPDYRGSDERKYFVLPVPYFVYRGDFFKADREGVRGVLFDSERIELDLSAALSPPVD
                     SRDNRARAGMADLEPVAELGPNLDVLLWENSDARLKFKAPLRAAFTLEGSPRGVGWVF
                     SPRLDLDFHNVAASGWRMGLLAGPIYATRANHDYFYSVPAADATATRPRYQAGGGYAG
                     TQVLASLSKRFPKFWVGAYLRWDTLAGATFVDSPLVTSRDYLAGGLALSWVFGESMQR
                     VEATP"
     gene            12959..13756
                     /locus_tag="VIS73_07630"
     CDS             12959..13756
                     /locus_tag="VIS73_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5763062.1"
                     /translation="MKALRTAYEYLVLYGGLALLGIGCLAWSLPAWLLHPLLPRATGR
                     RLGRLAIQKLFRAYLWVLEASGTCRFEVDALDQLRDEEAMIVAPNHPCLLDAVIVLSR
                     LPRAVCIMKAALMANPMFGGGARLARYIRNEPLVGMAVQAVAELKAGAHLVVFPEGTR
                     TTAWPLNSFKGGFAIAAGRAGVPVQTVFIETDSPFLSKGWPLWKKPAMPLVYRVRLGR
                     RFLTTGDTQGFLRELHVYYREELAGATLPNSAIGAGKVACGASEVGA"
     gene            13753..14517
                     /locus_tag="VIS73_07635"
     CDS             13753..14517
                     /locus_tag="VIS73_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5763063.1"
                     /translation="MSAAASLTHLVLIPSYNPGEKVYETVRAARAQWQPVWVVDDGST
                     DGTAAGLKAMADEDAGLRVIVLPRNRGKGAAVLLGIEHAAAEGFTHALTMDSDGQHPA
                     ELIPEFMRASREAPAAMVLGKPVFDASAPTLRVRGRRISNWWANLETLWAGIGDSLYG
                     FRVYPIDPLRRVMRGQRWMRRFDFDPEAVVRMCWRGVRPVNRPAPVRYFRPDEGGVSH
                     FNYLRDNALLTWMHTRLFLGFLLRLPLLAARRLRST"
     gene            14514..15290
                     /locus_tag="VIS73_07640"
     CDS             14514..15290
                     /locus_tag="VIS73_07640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5763064.1"
                     /translation="MKTETPVSTPVVHAPHVPLTDYYADEDARPGFVRNLFDKTAPDY
                     DRIETLLAFGSGRWYRGQALGRAGLTAGMRVCDVGIGTGLVAREAIKLVGDPALIVGV
                     DPSVGMMEQARGLGVQLVEGRAESIPFPDATFDFLSMGYALRHISDLEVAFREFRRVL
                     KPGGRLCLLEITKPEGRIASTLLKAYMKGVVPVLARCVGASSETAHLWRYYWDTIEAC
                     AAPERVLATLEAVGFDGVRRHIETSSLSIFAEYQAASPAA"
     gene            15501..15887
                     /locus_tag="VIS73_07645"
     CDS             15501..15887
                     /locus_tag="VIS73_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY5763065.1"
                     /translation="MSEPNPCLDCGVCCTHFRISFYWAEADDAGGTVPANMTEHLQQH
                     LRCMKGSNQTPRRCAALLGEVGQGVRCTIYPQRPSPCREFPVFHDDGTPNEACQRLRA
                     TIGLPPLAPLAPLHPLTPSPDLPRAA"
     gene            16210..17538
                     /locus_tag="VIS73_07650"
     CDS             16210..17538
                     /locus_tag="VIS73_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEY5763066.1"
                     /translation="MLTLNGAVSPATADYLLRGMRTAADRKASLVVIKMDTPGGLDTA
                     MRDIIKAILTSPVPVATFVSPSGARAASAGTYILYASHVAAMAPGTNLGAATPVAIGL
                     PGQPPAPKAGDEDKASDAKKADADGSDKDKKAAPADAMSAKQVNDAAAYIRGLAQLRG
                     RNSEWAELAVREAASLSAEDAVGSQVVDLIALDIPDLLKRIDGRTVSVEGLERTLKTS
                     PVELQVLDPDWRSRLLAVIANPGVALILMMIGIYGLIFEFSNPGFVVPGVVGAICLLL
                     AAFAFQLMPINYAGLALILLGIGFLVGEAFMPSFGILGIGGATAMIIGSVMLFDEEAS
                     AAYALPTSFVVALGAVSSALVFATVALALKARKRPVVSGREDLLAATGIALEDFTGEG
                     WVRVAGERWRARCTSRLHSGQVLRVTALDGLKLSVEPAGEANGEPGGDKK"
     gene            17535..18329
                     /locus_tag="VIS73_07655"
     CDS             17535..18329
                     /locus_tag="VIS73_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEY5763067.1"
                     /translation="MIWEYSYMALIVVLALLLVSALRILREYERGVIFMLGRFWKVKG
                     PGLIILIPGIQQMVRVDLRTVVMDVPSQDVISRDNVSVKVNAVVYFRVIDPKKAIIQV
                     ENFFEATSQLAQTTLRAVLGKHELDEMLAERDRLNSDIQQSLDAQTDAWGIKVATVEI
                     KHVDIDQSMVRAIAQQAEAERTRRAKVIHAEGELQAAEKLLQAAQMLAQQPEAMQLRY
                     LQTLGAIAGDKSSTIVFPVPIDLLGALTGQKSGAGSGGKTESPRPE"
     gene            18502..>18902
                     /locus_tag="VIS73_07660"
     CDS             18502..>18902
                     /locus_tag="VIS73_07660"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HEY5763068.1"
                     /translation="MNRRQSLASLLGISLVSLGGYRWASAASKEGFVVMKSKAEWAKL
                     LPPDSYRVLFEEGTERAGTSPLNREKRDGTYLCAACNNPLFDASKKYESGTGWPSFWE
                     ALPGALGTKTDYILLYPRTEYHCARCGGHQG"
BASE COUNT         3052 a         5960 c         6425 g         3465 t
ORIGIN      
        1 gtcgaagttg gcttggcgaa ccagcttttc ctcgtactcc ttgcgtaccg agatgtcttc
       61 cttgatcccg aggtactgga tgatccggcc gccctcgtcg aagatcggcg aaatcgtgac
      121 gttctcccag tagagatcgc ccgccttgtt gcggttgtgc agctcgccct gccagcgctt
      181 gccggcgcga atggtttccc acatctcgtt gtaggtctgg ctcggcgtca ggcccgactt
      241 gatgcgcgcc ggagtctcgc cgatcaattc gtcgcgcgtg tagccggtgc tctcgcagaa
      301 acgcggattg gcgtactcga tgacgccctc gagattggtg atgacgacgc tcaccgggct
      361 ctgctctacc gcctgcgaca ggcgattcat gtcgagttcc tggcggccca gcgtttccag
      421 catgccgttc agtgttgcgc ccagccgggc cagttcgtcg ttgccgcgga tttcggcgcg
      481 cgactgcttg tcgccggtct gacggcgacg catcgcctcg ccgattgccg ccagcggtgt
      541 cagcacccgg cgtgtcatca gcaagaaggc tatcagcatc gttacggcga ccgctgccga
      601 catgccgctg gctatgcgca aggtgttggc gcgaaccagt tcctcgaccg gcttgcggtc
      661 gagcaggata aggatttgtc cgcgtgcctt gtgcgtgtca cgggcaagga agccgggacg
      721 ctcgccgtag tgccctgcaa ctgcaggcgg tggcgggtcg gcgaccgcaa cggccgggct
      781 gccgtgatcg tggcgctttc cgtcggcgtg gatgtgcacc ttgcccgggg caggcgccgc
      841 cctagcgccc aaatgatcga tgacgctggt gctgtgctcg tgatcgtgat ccgaaaggct
      901 tgcctcgagc ggcatgacga tctgccggta gcgaccgtcg gccgagacgt cgctgcgaag
      961 catttcaccg ccggtagacg cggatgcatt tagtccctgg acgagttccg ggtcgggaag
     1021 cgaactgacc ttaaggccga tccattcggg gatcgtcgcg gcgctgacct tgccgtcgct
     1081 gaccagcagc gccaacgaga gcttgccggc gtccttgtag acttcctcga cgacgtgctg
     1141 ctcttcgctc gcattgtggg cgaccatcgc ggcgtggttg agtgcgctgg cgatcgccac
     1201 gccttcttcg agcagcgccc ggtcggcttc ggcgagcgtg gtccggtagg cgtagacaga
     1261 accgatgagg ctgacgacga ccccgccgag cagcagcggc atcagaaagc ggtaacgcag
     1321 cgaatcgttc agggacatgg cggcggacgt tggcggtaaa ggtcagtcga cggctgcgtc
     1381 ggaaacggcg ggttgccgcg ccgcgcgatt gtacgggttt gatctcgatc cggcagccgc
     1441 aatcccagtc ggcggcattc tgcgcgacaa tcgcgtctcc aaaaaatctg gtggatcgga
     1501 tggaacgagt gaacggaaag gaaaggcttg cgatgcgtgg cgcgctgctg atggtggcgg
     1561 cggtgctgct ggcggcctgc ggcgagcggg gtgatgcgga tggcccgagc tgggaagaac
     1621 tcgcgcacgg cgaggctatc taccgggccg agtgcgcgca gtgtcacggc gccaatctcg
     1681 aagggcagcc cgactggcga gtgcgcaagg ccgatggcaa gttgccggcg ccgccgcacg
     1741 atgcgagcgg acacacctgg caccactcgc gggagcagct gttcgccatc gtcaagttcg
     1801 gcatggtgcc gccgaatgcg cccgagggct acgtttcgga catgccggcc tacggcgcca
     1861 agctcagtcc ggcgcagatt cacgcggtgc tcgcctacat cgagagccgt tggccacccg
     1921 aggtgatcac gcagcgcaag gaacgcttcg cgcaggcgac ggccgaaaac gcgacgaaaa
     1981 aatgacgacg ggggccgaag cccccgccga tagccattct gtttattgga cggctatatc
     2041 ctcagcaggc cttaagcggc cattgcgaaa cgctcatcgt tggcgtttgt ggttttgcgc
     2101 ggattacgtc cgtcgcctct cgggccgcct gctcaccttc tgtcaccctg tcgaaaccag
     2161 tacacccccg ctgatgtgtt gcggaaagtg gtggaggtgg cgggaatcga acccgcgtcc
     2221 agaacgcttc cagtttgccg gaattacgac cgtgggttga ttatgggggc cgcgggcgcc
     2281 gatatcaagg cgtctggcga attgatcggc tcagaggccg cgcgcccagg cgggccgcag
     2341 cccggccgtt tcggggctgg ccagtgcttc gtcgatgcgc gcgagcaggc gcgccttgtc
     2401 ggtacccagc gtgcccttga ggatcagcca gttggaggcg tggtcgctgc gaaaaacggt
     2461 tcgcgtcagt tccagggcgg cgatgaagcg gcgcatctcg gcgatcaggc cgcgtgtgtc
     2521 gagcggttcc cattcgggga aggcgtcgcg caggcgttga tctccctgcg gaaagctcac
     2581 taccagcgtt gccagatatt cgggttgcgt ggcgttgatc aacgccgccg aatcatcggc
     2641 gtgggcgtcc gatagcgccc tgccgcccaa gccgttaagg atcatcaccg agcgcgtgat
     2701 gccggcggca ccgagccggt cgagcgcctc gcgcgtgctc tcgcgggtct cgcccttgga
     2761 cacgcgttcg agcaccgtgt cgtttccgga ttcggcgccc acgtagacga gcttgagccc
     2821 ggcctcggcc agtgccttca ggtcggcatc ggatttcttg cgcagattgc gcggcaggca
     2881 gtacgaggaa atccgccgga cggcgggcag gtgggtggcg atcgcctcca gggtttcgag
     2941 cagtcgccgc ggtggcagtg ccagcgcgtc gccgtcggcg aggaagacgc ggcggacctg
     3001 atcgccgtag cgttcaccgc aggcgcggat ggtggtaagc acttcgtcgg cgctgcgggc
     3061 gcgaaagcgt ttctgcggtg ccgtgtacat ctcgcagaag gtgcagtgat tccacgagca
     3121 gccgtcggtg agcggcagga tcagcgattc ggcttcgctg ggtggacgaa agacaggttc
     3181 tacgtaacgg atcgggaagt ccggcgccat gttgtctctg cgggctggtt cggcgaggac
     3241 cgcaggatag cgcagcgaag gtttagccgt tggtcttctg gctggcgagg aagcgccgca
     3301 actcgtcgaa gggcagcggt ttgctgatca ggtatccctg cagggcatgg caaccacctt
     3361 cgaccaggaa ggcgcgctgg tcgtcggtct caacgccctc cgcaaccacc tcgagtcgca
     3421 ggctgcggcc gagcgcgatg atcgcggcgg tgattgccgc gtcgtcgtcg tcgatatgga
     3481 tgtcacggat gaaggactgg tcgatcttca gcttgtcgat cgggaaacgc ttcaactggc
     3541 tcaggctcga atagccggtg ccgaaatcgt cgatcgaaaa ggtgatgccg gtctccttga
     3601 gcgccttcat cgtgcggatc acgtgctcgg gtcgttgcag gataaggctc tcggtcagtt
     3661 cgagttcgag gctggcagcc ggcaggtcgg ccaggcgcaa cgcgcggcgg acacgatcgg
     3721 cgaaatcgtc ctggttgaac tggcgcgccg agacattgac ggccatgcgc agttgcggga
     3781 agcctgcgtc ggcgagtgcc ttcgcggcga ggcaggccga acgcatcacc cagcagccga
     3841 gttcgacgat cagtcccgtg gtttctgcaa tcgggatgaa cttgcccggc gggaccatgc
     3901 ccatttccgg atgcgaccag cgcaccagcg cctcgagccc gacgacgcga cctctcgcga
     3961 ggtcgatctg cggctgaaag tggagaacga actcctcgcg ctcgatggcc tggcgcaggc
     4021 gcgattcgag cagcaggcgc tccatagccg cggcgttcat ctccgagcgg aagaattcgt
     4081 aacgttcgac accttcgttt cggacgtgat ggagcgcggc gtcggcgtgc ttgagcagtg
     4141 tcgcgccgtc gtggccgtca ccagggaaca gggcgatacc gatatgggcc tcgacatgca
     4201 gcggatgcgg gtcaagttgc atcggcgctt ggatcgtctc cagcagcttc tcgacgcgat
     4261 gccgtgtcgc agtcgaatcc tcgatatcgt caacgatgat cgcgaagcca tcaccggcgt
     4321 ggcggctgac gatttcgctt tcgctggtcg ccgccgacag gcgctgcgcg gtagccagaa
     4381 ggaccttgtc gccgtactgg tggccgagcg tgtcgttgat gtggctcagg cggtcgatgt
     4441 cgatggtgag cagcgcaaag cgcgaagact cctgcttggc gcgagcgatt gctgcgtcga
     4501 tgcgcaccat cagcaaggca cggtttggca ggccggtcag cgcgtcgaag taggagagat
     4561 gctcgatctt gcgttcggcc tcgcgcgcct cggtgacgtc ggtaaagatg ccgatgtagc
     4621 cgctggcatt gccgtccgca tcgtcgatgc gggtgaccga taggtgttcg gtgtagagac
     4681 cgccgcaggc cttgcggttg atgacctcgc cactccagtg gccgcgttcg cgaagcgttc
     4741 gccacatcgc tgcgtaaaag gtcttgtcct gcgttcccga gttgagcaga gtcggcgtgc
     4801 ggccgagcac gtcgacctcg cggtaaccgg tgatccgcgt gaaggcggca ttgactcgca
     4861 ggatgcgggt gctcgcgtct gtcagcatga tgccttcggc gctgcgctcg aagaccgtgg
     4921 acgagaggcg cagcgcttct tcggttttct gtcggtcgct gatgtcggta taggtggtaa
     4981 tgaacccctt ggtctcacca tcgatcaaga gcggcttgcc gcagaccaga tgcgtcttgc
     5041 cgttgggcct gacacgttcg aactggtgcg gctggaagcg catcgccaag gcatgaattc
     5101 gcgccacgtg ctgctcggga tcacccgggc cgtattcgcc gcgtgcagcc ggaatgcgga
     5161 tcaggtcgtc gaagtgaacg ccttcgcgaa cggcatccgc cggcagttcg agcacgtcga
     5221 tgaaaccttg attccagtgg cgcaggacca ggtgctcgtc gaagacactg attccctgtg
     5281 gcatgtgttc gacaaccgcg cgcagcaggg ccgattcgcg agcgaggtcg gcgcgggcct
     5341 gacgtgcctc ggtgacgtcc gaatagatcg tgacaaagcc gccgccgggt agcggctcgc
     5401 cgcgaacctc gatcaccgtg ccgtcagggc ggactcgatc gaaatgatgt gcctcgttga
     5461 gggttgtccg gcgcacccgt tcggcaacca gcgcatcggc ctcgcccggg ccgtattcgc
     5521 cgcgctcggc gttgtagcgg aacagagttt ccagcggtac cggtccggcg gcaaagaagc
     5581 tgtcggggag ccccatcacc cttcgtgcgc gctcgttgca gaggatcaca tctagattgc
     5641 ggtcgatgat cgaaatgccg ctcggcatcg tgtcgacgac ggtgtcgagc agctcgcttt
     5701 tctgcctgag ttcgcgatcg agccgcttct ggtcggtgac gtcgagcatg acgccgacca
     5761 agccgccttt ggcgcccgct tcgcgtgtga agatcgcctt gtagaagacg acgtcgtgtc
     5821 ggctgtcgtc ggcgtactga acctgtgctt cgtaaacctg cgtgccaccg gaaccgagaa
     5881 gtttgcggtc ggcctcctca tagaggcgcg cgaggtcagg tggcgcgatg tcgtagacgg
     5941 tcttgccgat cagcgtgccg cgagcgatgc cgatgtagtc ctcgaaggcg cggttgcaac
     6001 cgccgtagcg gccgtcgcca tccttccaga agatcgggca ggggatcgcg tcggcgagtt
     6061 gccagatgaa atggaactgc tcggcgatcg cctgctgggc ttgcttttgc gcggtgatgt
     6121 cgaccatgat tccgcgcaag gccgacggta tgccgttgcg aacctcgacg ctgaccgagt
     6181 ggcgcagcca gacagtttgt ccgtcggcgg cgagcattcg atattcaaat acgtgatcga
     6241 cgccgccgcg gcttttttcg gtgcagaact cgaccgccgt gtcacgatcg tcggggtgga
     6301 tgtggtcagc ccaaaagcca agggaaagcc agtcgctggc cggataaccg agcaggcgct
     6361 cggcgtggcc gctgacgaag gtgaagcgga aggttgcggg gtccgcctcc cagacgatgc
     6421 cgtcgatcgc ttcgacgagc gcttcgaggc gatgcaggcc ggccgatggg tttccggctg
     6481 cagatctggg aaaagggtga acgctgctgt tcatccaagt atcacgaaag taatagtcgg
     6541 acgctagggt agctccggtc gatgccgatg gcaagtccag acgcaatggg aaggatttag
     6601 aggctaagga gcagtttcag ttctgttggc gaatcgatca ggtcgtcggc attccaggcc
     6661 tcgattggat cgtcgacgcc gagatatccc cagcgcacgg cgatggtgcg cataccggcg
     6721 gcgcggcctg cgtcgatgtc gcgcttgtcg tcgccgacgt agcagcagtc gcgcgggctg
     6781 accccggcga gttcggcggc atgcagcagc ggcagcggtg acggcttcgc ttcgggcgtg
     6841 gtatcgccgc agaccaggca tgcgagccgc tggatcaggc gcatgcgttg catcagcggc
     6901 cgggcgaagc ggctcgtctt gttggtgacg accccccagg ggatatgccg cgcttccagc
     6961 gcctcgagca cgtcggtcat gcccggaaag agcaccgtct gctcgcacag cgccgcttcg
     7021 tagaggtcta ggtagtgggc gcgcagcgat tcgtactcgg gcgcggttgg tccgatgtcg
     7081 aagccggccc gcaacatgcc gcgcacgcct gacgaggtat gcgggcgcag ctcggcgagc
     7141 ggcagcgccg gcaggttgtg tgcggcacgc agccggttga gcgcgttgcc gaggtcgggt
     7201 gcggtgtcgg cgagcgtgcc gtcgaggtcg aagaagacgc cggccgtcac gcgctcaggc
     7261 cgggcgccgg gcgcgcagca ggtaattgac ggacaggtcg cggccgaggc ggtaggtctg
     7321 ggtcagcggg ttgtaactca tgccgatcag ctcggcgacg tcgagcccgc tgtcgcggca
     7381 ccagcgtgtc aactcggccg ggcggatgaa cttctggtag tcgtgcgtgc ccttcggcag
     7441 catccgcaac aggtactcgg cgccgatgat tgcgaacagg taggactttg gattgcggtt
     7501 aagcgtcgag aagaacaggt ggccaccggg acggaccagc cgggcgcagg cggcgacggt
     7561 ctgcgccgga tcgggaacgt gttcgagcat ttccaggcag gtgacgacat cgaagctgcc
     7621 cggcgcctcg tcggccagcg cctcggcggc gaccagccgg tacttcgccg gcgagccgct
     7681 ttccagcgcg tgcaggcggg cgacgcccag cgccttctcg ccgaggtcaa ttcccgtgac
     7741 ctgcgcgccc attgctgcca ttgcttcgct gagcaggccg ccgccgcagc cgacgtcgag
     7801 cagcgttgac tggtcgagtt tcgttccggc ctgctcgacg gactcgcgga tgaattcggc
     7861 gcgcaacggg ttgatgtcgt gcagcggctt gaactcgctg ttcggatccc accagcggtg
     7921 cgcgagctcg gcgaatttct cgagttcgcg caggtcggcg ttggcgttga acggtttggt
     7981 gtcggttgtc atcggtttcg cgtccagtgg gttcgcgtcg gaaagtgtcg ggtggcgatt
     8041 cagtgcgccg cggcgatgcg ctcgccccag ctttgcgcgc ggcgggccag tacgccatcg
     8101 tcgactgcga gcaggcggcc actggcgacg cgtcgccgac ccgccaccca gacatcgctg
     8161 acctgttcgc gtccggcgac gtaggcgagg tgtgaagcga cctgatagca gggcagtgtt
     8221 tcgggatcgg cgaggcgaat cgcgcagagg tcggcggctt tgccggcaac taaagagccg
     8281 atcagcgaat ccaggcccag cgcccgggcg ccgttgatcg ttgccatgcg cagcgccgcg
     8341 tgggcgtcga ttgccgttgc gtcgcccgaa acgcccttgg ccagcagtgc ggcgagtcgc
     8401 atttcgccga gcaggtcgag ccggttgttg ctcgctgcgc catcggtgcc gaggccaatg
     8461 ttaatgccgc gcgcggccag atgcgtgatc ggcgcgatac cgctggcgag cttgaggttg
     8521 gaactcgggc aatgggcgac cgagacgttg tgcgcggcga gcaggtcgac gtcggaggca
     8581 tcgaggtgca cggcgtggac tgcaatcgtc gatgggccga gcaggttcag ccgggccaaa
     8641 cgggccagcg gacgctcgcc gtgctgggcg atgctgtcgc tgatctcgct ggcggtttcg
     8701 tgcacatgga tgtggaccgg caggtcgagc tgctcggcga gcgtgaccac gcgagtgaag
     8761 gtttcgtctc cgaccgtgta cggcgcgtgc ggtgcgaggc agaagtgcac ctcgggcagc
     8821 tcgcggcagg cgtcgcgcac ggccagtccc ttgcgcaggt agtcgtcagc gtcggaggcg
     8881 tagggcgtcg gaaactcgag cgtcgcgatg ccggcgacga agcgcatgcc gacggtgccg
     8941 gcggcttcga tcgccgcgcc cggaaagaag tacatgtcgt tgcagcaggt gatgccgccc
     9001 ctgagcatct cggcgcaggc gagcagcgtg ccgtcgcgga cgaaggcctc gctgacaaac
     9061 cggccttcgg ccggccagat gtgctcggac agccaagtca tcagcggcac gtcgtcggcg
     9121 atgccgcgca gcaggctcat cgccgcgtgc gtgtgcaggt tgacgaggcc cggtagcaga
     9181 acctgatcgg gcaggtctat cgtttcgtcg gcggtgaagc gcgacatcgc gtcggcggtc
     9241 ggcagcaatg cgacgatgcg cccggcgcga acggcgacac tgtgccgttc gagcacgaca
     9301 ccctcgggtt cgatggggat gatccagcgg gcagcgatca gcaggtctac agattcggtc
     9361 atggtgtccg atagcacata agggaaaccc cgcctgagcg gggtttccgg actcgctgcg
     9421 attgtagccg aagcgtgttc agggcttggc gacgccgtcc acgtcaatcg tgatgcgccg
     9481 gaacgcggcg tagcaggcaa ccagcttcgc gcgcgactga tccttcgcgc agctgacgcc
     9541 ctccggcggt tcggccgaac caacaccgcg ggtggtgacg accttcgcat cgacaccgcg
     9601 cgacaccaga tactccttca ccgcggcggc ccggttccgc gacaggcgct cgttgccgct
     9661 cgccgagccg agtggatcgg tatgaccgac gatctcgatg cgatcaatgc ggctgaagcc
     9721 cttgagcttc tcgagtacgt cgcgatcgat gtcggcgcga ccgacgtcgg tcagccgcga
     9781 actgccgatc gcgaactgcg ccgaaccgct cagttcgatc cgcttcggct tcggcggggg
     9841 cgggggcggg ggagggggag gcggcggggc gaccacgacc ggcgccggcg cgggagcggc
     9901 gaccggggcc ggcttcggcg gtggcggcgg gggcggtgtc atcgcccgcg gcggttccgg
     9961 cttcttcatt cccggttcgc attcggggat ggcatcggcc ggcgtccaga atccggtgcg
    10021 ccagcattcg ttaaaggaat tgcggacgac ggtgccgttc gagtcgacga cgtagccgtg
    10081 cgcgatcgcc tgtgtggtaa cgatcgccag cgagcagctg gcgagataaa ggttgatttt
    10141 cttcatgggt cctcccgagc gggtcaaaaa aaaccccgcc gtggcggggc ttttcatacg
    10201 accgttcgaa aaacggagag ttcccaatca gcccggagcc atgcccttca gctcgacctc
    10261 gacgcggcgg ttcggcgcca ggcactcgat catccgctcg cgcggcaacg agtcggcaca
    10321 ggacaccccg ggaaccggct gcgtcttgcc ggcgccgagc gtctcgatga tgtcggcgtt
    10381 gacgcccttc atcacaaggt acgccttgac cgcgtcggcg cgcgcttcgg agagctgctg
    10441 gttgtactgg tgcgagccca tgcggcaggc gtggccggtg accgaaacga agtcagtgct
    10501 gttaagcgtg ccaagcttcg agaggatgtt cgcgtcgatc tcggccttgc cgtcggcggt
    10561 aagcacggcc ttgtcaaatt cgaacatcga cttcgacttc agcgatacct ttttcggctt
    10621 cggtgcctcg acgggcttcg gcgcctcaac gggcttgacc ggctcgggcg ccggcatcgc
    10681 acgcggcgcg ggtgccgggg cttcttcctt cttctctggc gtcgcacagg ctccgaccga
    10741 aaggatcaac gcgacgatcg cggcctggcg gaatttattt tcttgtttca aagcggctcc
    10801 tccttgtatg gatttcggga aaggcttccc gaatatcgtt catcgtaacc gcgggtggcg
    10861 gaacaccgtg attacaacaa acaatcatcg gggcaacaag tacactgctg atttgccgcc
    10921 aatcaaatgt ttttaaagag aaatggcttc actggcgggc gcggccagcg tatccgccgc
    10981 tggcgcgatg cgataaaatt cgccggctat gccttcgacg cccgccgccg tgcccgatcc
    11041 cacccttccg tcctgctcgc gcgagtgccg cgacagcacc tcggcacttg agtttgtttc
    11101 ctgctcgggg atcgccgatt gcggcaagtg cttgaacgac agccactttc tcggcggcgc
    11161 cttctttggc gcgccgctag cggcggcaac gaaggcgctg ccggtcgtgc acgtgccggt
    11221 gccggtcttg gccggcgacc cgagcgaggg cgagtgctgg ctcgcggcag ggccggccga
    11281 agcgggcgat gacccggcca ccggcttgcg ctttcgccac gacggcactt tcgtcttcgg
    11341 tgtcgtccgc ctgcgcgagg ccgacttcgc cggtggcgat ggctcgccgc tgcagcaggc
    11401 gaccgcggcc gcctacggtg ccgtgttcgc gacgatggac aggctcggct acccgcatct
    11461 ggtccgttgc tggaactacc tcgccgacat caacggcgaa ctcgacggcc tcgaacgcta
    11521 ccgccatttc aacatcggcc ggcaggatgc gttccttgct gccgctcgcg acacggtggg
    11581 cagcgttccc gcagcctgcg cgctcggcac ccgtgacagc gacctgttga tctgtttcct
    11641 cgccgcccga agcgcggcgc tgccaatcga gaacccgcgg caggtttccg cattccgtta
    11701 cccgcccgac tacggtccgc gctccccgac ctttgcccgc gccgcactgc tgccgttgcc
    11761 cgggcggcca ttgctactgg tttcgggcac cgccagcatc gtcggtcatc gcaccgtgca
    11821 ttccggtgac ctgtgcgcgc agttgcgcga gtcgatggcg aacatcgctg ccgtcctcgc
    11881 cgaggcaaca cggcaggcgg caggcgccga gttccggctc ggtggtctcg actatcgcat
    11941 ctacctgcgt catgccgacg acctcgcctc ggccaagcag gccttcgggg cgcattttgc
    12001 cggtatgccc aagccgcacg ccgtgttcat gcaagccgac gtctgccgcc acgatctgtt
    12061 ggtcgagatc gaggccagcg gttacgctgg cccctgatgc cgatcgaccg gccgctgcgc
    12121 atcgggacgt tgcgcgtcgc tttcgcgctg acgctgcttg gcatggtgtc gccgctggcc
    12181 gccgaacaat taccgctttg ggaggccggt gccggcgtcg gcgtgctgtc gctgcccgat
    12241 taccgcggca gcgacgaacg gaaatacttc gtgctgccgg ttccgtactt cgtctaccgt
    12301 ggcgacttct tcaaggccga ccgcgaaggc gttcgcggtg tgctgttcga cagcgagcgg
    12361 atcgaactcg acctcagcgc cgcgctctcg ccgcccgttg acagtcgcga caatcgtgcg
    12421 cgcgccggca tggccgacct cgaaccggtc gccgaactgg ggccgaacct cgatgttctg
    12481 ctctgggaaa acagcgacgc gcggcttaag tttaaggcgc cgctgcgcgc cgcgtttacg
    12541 ctggaaggtt cgcctcgagg ggtcggctgg gtgttctcgc cgcggctcga tctcgacttc
    12601 cacaacgtcg ccgccagcgg atggcggatg gggctgctcg cgggaccgat ctacgcaacc
    12661 cgggcgaacc acgattactt ctattcggtt ccggcagccg acgcgacggc tacgcggccg
    12721 cgctaccagg ccgggggcgg ctacgccggc acgcaggtgc tcgcctcgct gtcgaagcgc
    12781 ttcccgaaat tctgggtcgg cgcctacttg cgctgggata cgcttgccgg cgcgaccttt
    12841 gtcgacagcc cgctggtaac cagccgtgac tatctggccg gcggactggc gctgtcctgg
    12901 gttttcggcg aatcgatgca gcgcgtcgag gcgacgccgt gatgcggagg gcctgtcaat
    12961 gaaggcgctg cgcacggcct acgagtacct ggtgctctac ggcggccttg cgctgctcgg
    13021 catcggctgc cttgcctgga gcctgccggc ctggctgctg catccgctgc tgccacgcgc
    13081 taccgggcgg cgcctcggca ggctggcaat ccagaagctg ttccgcgcct atctctgggt
    13141 actcgaagcg agcggcacct gccgtttcga agtggacgcg ctcgaccagc tgcgcgacga
    13201 ggaggcgatg atcgtcgcgc ccaaccatcc ctgcctgctc gatgccgtga tcgtgctctc
    13261 gcggctgccg cgcgcggtct gcatcatgaa ggcggcgctg atggccaatc cgatgttcgg
    13321 cggtggcgcc cggctggcac gctacatccg caacgagccg ctggtcggca tggccgtgca
    13381 ggccgtcgcc gagttgaagg ccggcgcaca tctggtcgtc tttcccgaag gcacgcgcac
    13441 cacggcttgg ccgctcaaca gtttcaaggg tggcttcgcg attgctgccg ggcgcgccgg
    13501 cgtgcccgtg cagacggtct tcatcgaaac cgactcgccc tttctcagca agggctggcc
    13561 gctgtggaag aagccggcga tgccgctcgt ctaccgcgtg cggctcggcc ggcgtttcct
    13621 caccaccggc gacacgcagg gttttctcag ggaactgcat gtctattacc gggaggaact
    13681 ggccggcgcg accctgccga attcggcgat cggcgcgggc aaggtggcct gcggtgccag
    13741 cgaggtcggc gcatgagcgc ggctgcctcg ctgacccacc tggtgctgat tcccagctac
    13801 aacccgggcg agaaagtcta tgaaaccgta cgcgccgcac gcgcccagtg gcagccagtg
    13861 tgggtggtcg acgacggcag caccgatggc accgcagccg ggcttaaggc gatggccgac
    13921 gaagacgccg ggctgcgcgt gatcgtcctg ccgcggaacc gcggcaaggg cgcggcggtg
    13981 ctgctgggta tcgagcatgc cgccgccgaa ggtttcaccc acgcgctgac tatggattcc
    14041 gacggccagc atccggctga gctgatcccc gaattcatgc gcgcgtcgcg tgaggcgccg
    14101 gcggcgatgg tgctcggcaa gccggtcttc gacgccagcg cgccgacgct ccgcgtgcgc
    14161 ggtcgccgca tctccaactg gtgggcgaat ctggagacgc tgtgggccgg catcggcgat
    14221 tcactctatg gctttcgcgt ctacccgatc gatccgctgc gtcgtgtgat gcgcggccag
    14281 cgctggatgc gccgcttcga ctttgacccc gaggccgttg tccgcatgtg ctggcgcggc
    14341 gtgcgcccgg tcaaccgacc ggcaccggtg cgctacttcc gacccgacga gggtggcgtc
    14401 tcgcacttca actacctgcg ggacaacgcg ttgctgacct ggatgcacac gcgcctgttc
    14461 ctcgggtttc ttctccgtct tcccttgctg gccgctcgtc ggctgaggtc tacatgaaaa
    14521 ccgaaacccc tgtctccacc ccggtcgtcc acgctccgca cgtgccgctc accgactact
    14581 atgccgacga agacgcgcgg cccggattcg tccgcaacct gttcgacaag accgcacccg
    14641 attacgaccg catcgagacg ctgctcgcct tcggctcggg ccgctggtat cgcggccagg
    14701 cgctggggcg agccggcctg accgccggta tgcgcgtctg cgacgttggc atcggtaccg
    14761 ggctggtggc gcgcgaggcg atcaagctcg tcggcgaccc ggcgctgatc gtcggcgtcg
    14821 accccagcgt cggcatgatg gagcaggcgc gcggactcgg cgttcaactg gtcgaaggcc
    14881 gcgccgagtc gattccgttt cctgacgcca ctttcgactt cctcagcatg ggttacgccc
    14941 tgcgccatat cagcgatctc gaagtcgcct tccgtgagtt ccgccgcgtg ctcaagcccg
    15001 gtggccggct ttgcctgctc gagatcacca agcccgaagg gcgaattgcg tcgacgctgc
    15061 tcaaggccta catgaagggc gtcgtgcctg ttctggcccg ctgcgtcggt gccagcagcg
    15121 aaaccgcgca cctctggcgg tactactggg acaccatcga agcctgcgcc gcccccgagc
    15181 gcgtgctggc gacgctcgag gcagtcggtt tcgacggtgt gcgccgtcat atcgagacta
    15241 gttcgctgtc gatcttcgcc gaataccaag cggcaagccc ggccgcctaa gcagcgctgc
    15301 gcgggccgcg ccgccggtcg gtcgctgccc gcgcgatcgg cgcgaatcgc gccccatgcg
    15361 ggcctgcgag gcgaatgcaa gttgctgttt accggcgcga aaacatcctg ctagaatccg
    15421 ccgcccgctg ccgcgggggt cgctgatccg ccggcaatcc gggcctgtga aaccatctat
    15481 ctcagtgttg gcgggtgctc atgagcgagc cgaatccctg tctggactgc ggcgtttgct
    15541 gcacgcattt ccgaatttct ttctactggg ccgaggccga cgatgccggc ggaacggttc
    15601 ccgccaacat gaccgagcac ctgcagcagc acctgcgctg catgaagggt agcaaccaga
    15661 cgccgcggcg ctgtgcggcg ctgctcggcg aggtcgggca gggcgtgcgc tgcacgatct
    15721 acccgcaacg cccgagcccc tgccgtgaat tccccgtctt ccacgacgac ggcacgccca
    15781 acgaagcctg ccagcgcctg cgggccacga ttggcctgcc tccgctggca ccgctcgccc
    15841 cgctgcaccc gctgacgccg tccccggacc tgcctcgggc tgcgtgacag tggcgctacg
    15901 aggggctaga gccgtttagt cctcgacccg caggaatcgg ctggcttcca tcttcgtgcc
    15961 cccaatcgcg ccgggccgtt tcgcgatttg cttcggccgt ggcccccttg gtttgtccaa
    16021 cggacacgag cgcagttcgt ccgctgcaat ctcgggcgaa catccggacg cgtacggagt
    16081 caaagcttca tgcgcgtctc taggcaattc tttttgggac tattggtcgg cttggtaatt
    16141 tgcggcggat cggcgctcgc cgatcaagcg gcggatggtg ccaagccgag gcctcctgga
    16201 tcggtactgg tgttgacgct gaatggcgca gtcagtccgg caacggccga ttacctgctg
    16261 cgcggcatgc gcactgccgc agatcgcaag gcttcgctgg ttgtgatcaa gatggatacg
    16321 ccgggtggcc ttgacacggc gatgcgcgac atcatcaagg cgattctcac ctcgccggtg
    16381 ccggtcgcga ccttcgtctc ccccagcggc gcgcgcgccg ccagcgccgg cacctacatc
    16441 ctctatgcca gccatgtcgc ggcgatggcg cccggaacca atctcggcgc cgccacgccg
    16501 gtggccatcg gcctgcccgg ccagcctccc gcgcctaaag caggcgacga agacaaggcc
    16561 agtgatgcga agaaggccga cgctgacggc agcgataagg acaagaaggc ggcgcccgcc
    16621 gacgcgatga gtgccaagca ggtcaacgat gcggcggcgt acatccgcgg cttggcgcaa
    16681 ctgcgtggcc gcaacagcga atgggccgaa ctcgcggtgc gcgaggcggc cagtctctcg
    16741 gcggaagacg ccgtcggcag ccaggtcgtc gatctgatcg cgctggatat ccctgatctg
    16801 ctgaagcgga tcgacgggcg caccgtcagc gtcgaaggcc ttgagcggac gctgaagacc
    16861 tcgccggtcg agctgcaggt gctcgatccc gactggcgaa gtcgcctgct agccgtgatc
    16921 gccaatcccg gtgtggcgct gatcctgatg atgatcggta tctatgggct gatcttcgag
    16981 ttctccaacc cgggcttcgt cgttcccggc gtggtcggcg cgatctgcct cttgctcgcc
    17041 gctttcgcct ttcagctgat gccgatcaat tacgctggcc tcgccctgat tctgctcggc
    17101 atcggttttc tcgtcggcga ggcattcatg cccagcttcg gcatccttgg catcggcggg
    17161 gcgacggcga tgatcatcgg ttcggtcatg ctttttgacg aggaggcatc ggccgcctac
    17221 gcactgccga cgtccttcgt ggtcgcgctg ggcgcagtca gttcagcgct ggtcttcgcg
    17281 accgtcgcgc tggcgctcaa ggcgcgcaag cgcccggtgg tgagtggccg cgaagacctg
    17341 ctcgccgcca cgggcatcgc cctcgaagac tttacaggcg aaggctgggt gcgcgtcgcc
    17401 ggcgagcgct ggcgtgcccg gtgcacgagc cggctgcaca gcggccaggt gttgcgggtg
    17461 acagcgctgg atggattgaa gctgagcgtc gagcctgcgg gcgaggcgaa cggtgaacct
    17521 ggaggtgaca agaaatgata tgggaataca gctacatggc gttgatcgtc gttctcgcgc
    17581 tgctgctcgt ctcggcgctg cgcatcctgc gcgagtacga gcgcggcgtg atcttcatgc
    17641 ttgggcggtt ctggaaggtc aagggcccgg gtctcatcat cctgattccg ggcatccagc
    17701 aaatggtgcg ggtcgatctg cgtaccgtgg tcatggatgt gccgagccag gacgtgattt
    17761 cgcgcgacaa cgtttcggtc aaggtcaatg ccgtggtcta tttccgcgtc atcgatccga
    17821 agaaggcgat cattcaggtc gagaacttct tcgaagcgac cagccagctg gcgcagacga
    17881 ccctgcgcgc cgtactcggc aagcacgaac tcgacgaaat gcttgccgaa cgcgatcggc
    17941 tcaacagcga catccagcaa agcctcgacg cgcagaccga cgcctggggc atcaaggtcg
    18001 ccacggtcga gatcaagcac gtcgacatcg accaatcgat ggtccgggcg attgcccagc
    18061 aggccgaggc cgagcggacc cggcgggcca aggtgattca cgccgaaggt gagctgcagg
    18121 ctgccgaaaa gctgctgcag gccgcgcaga tgctggcgca gcagccggaa gcgatgcagt
    18181 tgcgttacct gcagaccctg ggcgccatcg ccggcgacaa gagttcgacg atcgtcttcc
    18241 cggtaccgat cgacctgctc ggcgcgctga ccgggcagaa gtctggcgcc ggtagcggcg
    18301 gcaaaacgga gtcacccagg ccggaataat ccagcctaga tagtgttctc cttcgtgcgt
    18361 gcgaaccgcc tgtaaggatt cgtcgcgcgg tccgactgat ggcacggaca agacgaacca
    18421 aacggcacca acaggcttcc aaagtgtgag ccgtttgccc ttggtttcgg cgagccgtga
    18481 aaatcatttc tggagaacga catgaaccga cgtcaatcgc tggcttcctt gctggggata
    18541 tcgctggtct cgctgggtgg ctaccgctgg gcgagcgctg cgtcgaagga gggcttcgtg
    18601 gtgatgaaaa gcaaggccga gtgggcaaaa ctgctgccgc ctgacagtta tcgcgtgctc
    18661 ttcgaggaag gaaccgagcg cgccggcacc agcccgctca atcgcgaaaa gcgcgacggc
    18721 acctacctgt gcgcggcctg taacaacccg ctgttcgatg cctcgaagaa gtacgagagc
    18781 ggtaccggct ggccgagctt ctgggaggcg ctgccgggtg cgctgggcac caagaccgac
    18841 tacatcctgc tctatccgcg caccgaatat cactgcgcgc gttgcggcgg gcatcagggg
    18901 ca
//
LOCUS       DATDAA010000240         9034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_332496,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000240 DATDAA010000000
VERSION     DATDAA010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9034
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332496"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VIS73_07665"
     CDS             complement(<1..509)
                     /locus_tag="VIS73_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HEY5763069.1"
                     /translation="MSLPLVASLLAGVSAMSVSCAARAEMRFNFPDPVTPIARDTLEI
                     HNEFMLVITVLFVVVFAIMIYSMAKHRKSVGHQPAKFTGPSGKIQWFWTLVPFGILLF
                     IDFVLMGIPAFHAVLDMEDTQTRADMVVKVTGLQWKWQYEYPGTEVKYLSTMTTPQAQ
                     IDNAEAKGE"
     gene            729..1271
                     /locus_tag="VIS73_07670"
     CDS             729..1271
                     /locus_tag="VIS73_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY5763070.1"
                     /translation="MAGCADRSPPPFGAEDITGASFGGDISLTDHRGNARSLADFRGN
                     AVLLFFGYTHCPDICPTTMSRFATVRRLLGDDGTRLKVVFVTLDPERDTVSVLEQYVP
                     FFDPDFVGLTGSVEAIADVAKRYRVPFVRRQAENGNYLLDHWAGAYAFDAEGRLRLYV
                     PPDLPNEALAADLRRLIAAK"
     gene            1298..1774
                     /locus_tag="VIS73_07675"
     CDS             1298..1774
                     /locus_tag="VIS73_07675"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY5763071.1"
                     /translation="MALLSRAWIGALFVATTAVADTGLVVESAWARATAPTASTGAAY
                     FVIRGGSADDQLLSATATVAERVELHTHTMADGMMQMRQLTEVAVPAGATVEFKPGGL
                     HVMLIGLKAPLAAAFSFDLALRFAKGGTRTVQVGVRGVGATGAETLPPGKAHGHQH"
     gene            1758..1955
                     /locus_tag="VIS73_07680"
     CDS             1758..1955
                     /locus_tag="VIS73_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763072.1"
                     /translation="MAISIDVRAVAFGLLLSSPALAATTVGDIDRYFEASRRLEAGEA
                     IAPRLHYQAGFYAGYLEATRE"
     gene            2166..2405
                     /locus_tag="VIS73_07685"
     CDS             2166..2405
                     /locus_tag="VIS73_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763073.1"
                     /translation="MQSCFARPSLACAIWRALLRGDDFGNAGMPGNGFDFFVAETTEI
                     NALIERNHRFPRKQVDWQRALLADESNRGDRASLR"
     gene            complement(2515..3393)
                     /gene="rpoH"
                     /locus_tag="VIS73_07690"
     CDS             complement(2515..3393)
                     /gene="rpoH"
                     /locus_tag="VIS73_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEY5763074.1"
                     /translation="MTAALIPAPQQGFSLSVPLSAGSLDAYIAAVNRLPMLSEAEELE
                     LGRRLRDDGDVTAARQLVLSHLRMVVSVARGYLGYGLPYADLIQEGNVGLMKAVKRFD
                     PERGVRLVSFAIHWIKAEIHEFVVRNWRLVKIATTKAQRKLFFNLRSMRETSDTLSPT
                     QLAEVAEKLKVRPEDVAEMEARFAGGDVGLEGSPDDGEDSFAPIDYLADGRYEPTRVI
                     AEKSRETLQSDGLQQALETLDPRSRRIVEARWLPAGEAATLHELAAEFGVSAERIRQI
                     EAKALTRMRGVLAEAA"
     gene            complement(3752..>9034)
                     /locus_tag="VIS73_07695"
     CDS             complement(3752..>9034)
                     /locus_tag="VIS73_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY5763075.1"
                     /translation="NWSRFCGGNSTAIGPAHEAAAALSKGAEQVGLTDFKRLTQALAT
                     ITKWVAEDKSRHNDAVAMETATAILLLSNTLDNFAHLGADFVHQVDVMVARLHACIGG
                     TPPTTGAEIPMLDEMTRLAQEKLLIAQVVREIQSNLGQIEQTLDTFFRDPEQRDQLKG
                     CQRPVAQIAGALTVLGQSDAVSALTHASERIAAFAAPGYQAQAADFEHVANQLSALGF
                     FVEALRSGNPDYQSFVAALGQRSAAPSAVSGEGFAASVEEAEAIATQTLSAEAKAAAL
                     TSPPPKAEAPEQPASPPTVEAPAISASVSAPPPTTTEPVAPATPEDSASDAEIDAELL
                     EIFLEEANEVLGTIAENLDLLKAQPSNVDALTTMRRAFHTLKGSSRMVGLRDFGEVAW
                     VVEQVNNLWLRQERTPDLNFFKLVQLSHEVFSAWVTHLASGAGSLPDPAALVAMAESL
                     KGGETVTLAQKAAPTAPAVVEEPPAAEETIDLGEAPENPDSELTLDLAPVDAETEPAT
                     DEALTISFDDAETDLQIDLGEVAESIDSLPAPYPAADASTEIDLGDLPAPPPELAPES
                     DEPLVAETGLPEITLDLPAPEADVEAPELAESSQSPELAADDISLDELAVPTVADQPI
                     RAIDAVPTADTHDHDESVPVLFQIFLEEARGHLETLTRAFGEIELDPTMPAPPDMIRS
                     AHTLGSIGGTVGFAVVKELGHGLESALLRRSHVRGPANLEALEVIREVIAELESMVVT
                     IASGQTPENRPLLVAGIASVYADAEPAENAEADLLAATSVIQPEAPESAGPVELPPDE
                     LDESLLPIFIEEAGDLVGAVNERLGMWRAKPDDVNTIHALARLLHTLKGSARMAGALG
                     IGALTHTLETMVEAAHHRGSADPEEIARFQLEFDEIADRITALRPESAAPDNALPTIE
                     IAESEIAAAEPPPAETTPSAVDITAPEAPPLIPEPPLSAAINPPLTPARSATSVPLPA
                     DSLDEDLLPVFLEESTELVKTIYAQLGAWRAAPADEGAQQALARALHTLKGSARMAGA
                     MSIGALTHTLEARVEELTKSGQADTGAIFDVQGGFDTIAGVIERLQQGEALSEITVDA
                     PGDAAAEPPAVATTPEPKAQTEAPSAAPHEATVTPISSAADDTAGIAPSAAAPIAPQR
                     AQGDALARPMLRVRAELIDRLVNDAGELSIARARIEGEMRGLKQAMLDLTENVIRLRR
                     QLREIEIQAESQLQSRIALTSETAASFDPLELDRFTRFQELTRMMAESVNDVATVQQS
                     LLKSLDESNAALANQGRLNRELQQALMSVRMVPFAGIVERLYRITRQTAKETGKRADL
                     EVHGSQIELDQNIINKMIGPLEHLLRNAVSHGIETPDRRVAAGKADIGEITLTLRQEG
                     NEVIITMQDDGSGLDLERIREKAIAKGLIGPQQEVEPRKLTDFIFHSGFSTATKVTQV
                     SGRGVGMDVVRSEVGDLGGRIEVQSDTGKGTTFRIYLPLTLAVTNAALVRIGSRTFAI
                     PASMIDQVMEVKVGVMTEMRDAGKVSWQGADYPYHFLPHLLGDTGALAEARKFYWVLL
                     LHSGAQRVAIQVDELTGSQEVVVKNIGPQLARVVGIAGATVLGDGRVLLILNPVALAA
                     RPPVTGPTVAAPSAAEAEETALIQLPTVLVVDDSLTVRKITSRLLAREGYQVVTAKDG
                     VDALQQMVEFVPDVILSDIEMPRMDGFELARNIRADERLREIPIVMITSRTAEKHRTH
                     ASELGVDHFLGKPYQEEELLKLVGDYTAHKRVTA"
BASE COUNT         1405 a         2823 c         3105 g         1701 t
ORIGIN      
        1 tgttcgccct tggcttcggc attgtcgatc tgcgcctgcg gcgtggtcat cgtcgacagg
       61 tacttcactt cggtgcccgg gtactcgtat tgccatttcc actgcaggcc ggtgaccttg
      121 accaccatgt cggcgcgggt ctgcgtgtcc tccatgtcga gcaccgcgtg gaaagccggg
      181 atgcccatca gcacgaagtc gatgaagagc aggatgccga agggaaccag cgtccagaac
      241 cactggatct tgccgctcgg gccggtgaac ttcgcgggtt ggtgtcccac cgacttgcgg
      301 tgcttggcca tcgaatagat catgatcgcg aacaccacga cgaagagcac cgtgatgacc
      361 agcatgaact cgttgtggat ttccagcgtg tcacgggcga tcggcgtgac cgggtcgggg
      421 aagttgaagc gcatttccgc gcgcgcggcg caggaaacgg acatagccga aacgccagcc
      481 agtaggctgg cgacgagcgg aagactcatc ggaccccccg agttgaaacc gctttgaatt
      541 cgcggattaa gattgtcgac aaacgaatga cacagagttc tatgaggcat accgtgatac
      601 agagttcccg tgctgcagcg cagaagatgc gtgtgcaggg ccggccattg ccggagcgtc
      661 tcctaacggc aatctcgcgc aggcttgggc gttggctcgc gcgcgtttgt tgcgtcgcga
      721 cggtggtggt ggcaggttgt gccgaccgtt cgccgccgcc attcggcgcc gaagacatta
      781 ccggcgccag tttcggtggc gacatcagcc tgaccgatca ccgcggcaat gctcgatcgc
      841 tcgccgattt ccgcggtaat gctgtcctcc tctttttcgg ctatacgcac tgccccgaca
      901 tctgcccgac gacgatgagc cgattcgcga ccgtccggcg gctgctcggc gatgatggca
      961 ctcggctgaa agtcgttttc gtgacgctcg accccgagcg cgacacggtg tcggtgctgg
     1021 agcaatacgt gccgttcttc gaccccgatt tcgtcggcct gaccggcagc gtcgaggcga
     1081 tcgccgatgt ggccaagcgc tatcgcgtgc ccttcgtccg tcggcaggcc gagaacggca
     1141 attacctgct cgatcattgg gccggtgctt atgccttcga cgcggagggt cggctgcgcc
     1201 tctacgtgcc gcctgacctg ccgaacgagg cgctggcggc tgacctgcga cgcttgatcg
     1261 ccgccaaatg aatcatcccc ttcgaaagga agctgtcatg gccctcctct cccgtgcctg
     1321 gatcggcgcg ctgttcgtcg cgacgaccgc tgttgccgat acgggcctgg tcgtcgagtc
     1381 ggcgtgggca cgcgcgaccg cgccaaccgc gagcaccggc gccgcctatt tcgtgatccg
     1441 cggcggcagc gccgacgacc agctgttgtc ggcaaccgcg acggtggccg agcgtgtcga
     1501 gctgcatacc cacacgatgg ctgacgggat gatgcagatg cggcagctca ccgaagtagc
     1561 ggtgccggcc ggtgcaacgg tcgagttcaa acccggcggt ctgcacgtca tgctgatcgg
     1621 gctgaaggcg ccgctggcgg cagccttctc ctttgatctc gcgctgcgtt tcgccaaggg
     1681 cggaacccgg accgttcagg ttggcgtgcg gggtgtcggc gcgaccggtg ccgagaccct
     1741 gccgcccgga aaggcgcatg gccatcagca ttgatgtccg ggcggtggcc ttcggcctgt
     1801 tgttgagttc gccggcgtta gcggcgacga cagtcggcga tatcgatcgc tatttcgagg
     1861 ccagccgacg tcttgaagcg ggcgaggcga tcgcgccgcg gctgcactac caggcagggt
     1921 tttacgcggg ctatctggaa gcgacgcggg agtgactgca ggccgatggt gcgcccggcg
     1981 gcgcagtggc tcgccgacct gctcgcgccg gaggtcgggc gcttgcccct gaggcggtag
     2041 tgcgcgcacg ctgctcaggc gcgggccgcg gagttcatcc ttttccgggc gccgggtcgg
     2101 ccgcggtggg cccggaccga cattcactcg cgaattcact agcctcggcc agtcgctggt
     2161 gcacgatgca gtcctgcttt gcccgacctt cgctggcctg cgcgatttgg cgggcccttt
     2221 tgcgcggcga tgatttcggg aacgctggca tgccgggcaa tggcttcgac ttcttcgttg
     2281 ccgagaccac ggagatcaat gcactgatcg aacgtaacca tcggtttcct cgaaaacaag
     2341 tggattggca acgagcgttg ttggcagatg aatcgaaccg cggtgatcgg gcaagcctcc
     2401 gttgatgagc tgcccgcctg cctctttctc ggggcaggat gctgatattc agggaaaaat
     2461 gaaaaggccg gtcgggtgac cggccttcat ggggaatcgc cgacgcggtt cgaatcaggc
     2521 tgcctcggcc agcacgccgc gcatgcgggt cagcgccttc gcttcgatct gtctgatgcg
     2581 ttcggccgag acgccgaatt cagcggcgag ttcgtgcagc gtcgcggcct cgccagcggg
     2641 cagccagcgc gcttcgacga ttcgccggct ccgtgggtcg agcgtttcga gcgcctgttg
     2701 cagaccgtcg ctttgcagtg tttcgcgtga tttctcggca atgacgcgag tcggctcgta
     2761 acgtccgtcg gcgaggtaat cgatcggcgc gaagctgtct tcgccgtcgt cgggactgcc
     2821 ttccaggcca acatcgccgc ctgcgaagcg ggcctccatt tcggcaacgt cttcgggccg
     2881 caccttgagc ttctcggcga cctcggccag ctgcgtcggc gacagcgtat cgctggtctc
     2941 gcgcatgctg cgcagattga agaagagctt gcgttgtgcc ttggtcgtgg cgatcttgac
     3001 cagccgccag ttgcgcacga cgaattcgtg gatttcggcc ttgatccagt ggatcgcgaa
     3061 ggacaccaga cgaacgccgc gctcggggtc gaagcgcttg accgccttca tcaggccgac
     3121 gttgccctct tggatcaggt cggcgtaggg caggccgtac cccagatagc cgcgggcaac
     3181 cgagaccacc atgcgcaggt gcgagaggac cagctggcgc gcggcggtga cgtcgccgtc
     3241 atcacgaagc cggcggccga gctcgagttc ctcggcttcg ctcagcatcg gcaggcgatt
     3301 gacggcggcg atatacgcgt caaggctgcc cgccgaaagc gggacgctca gtgaaaagcc
     3361 ctgctggggc gcaggaatca gcgcggcggt catggcttgc tcccttctga aagctggtga
     3421 aatgatttta gcactcgcac gaatagagtg ctaatagcgc ctcgggttcc gtcgcttgtg
     3481 ccaggccgcg gtccgtccat ggtattcgcc ggccgcgcgc ttgcctacgg gcaccgcccg
     3541 gtgggcaata aggggattcg ctggcccccg gtccaattga gccaacccgg gacctcgcga
     3601 gccgccgggc cgggcgactc tgccgaatac tgcggtttcc tgagcgcgga cggaagggcg
     3661 cccgaaaatc ccgccggccg gcccgggatg tgaatcgacc ccggagggaa ccgcgagggt
     3721 cccgggcgaa tccatacgcc cggggctggc gttaggcagt gacgcgtttg tgcgccgtgt
     3781 agtcgccgac cagcttaagc aactcctctt cctgataggg ttttccgagg aagtggtcaa
     3841 cgccgagttc ggaggcgtgg gtgcggtgct tttcggccgt ccgcgaggta atcatgacga
     3901 tcggaatctc gcgcagacgc tcgtcggcgc ggatattgcg cgccagttca aagccatcca
     3961 tccgcggcat ttcgatgtcg gagagaatca cgtcgggtac gaattcgacc atctgctgca
     4021 gggcatcgac gccgtccttg gccgtcacca cctggtaacc ctcgcgggcg agcaggcgcg
     4081 aagtgatctt gcgcaccgtc agcgaatcgt cgacgacgag tacggtcggc agctggatga
     4141 gggcggtttc ctcagcctcg gcggcgctcg gcgcggcaac ggtcgggccg gtgaccggcg
     4201 gacgcgcagc gagtgccacg ggattcagaa tcagcaggac gcgcccatcg ccgagcacag
     4261 tcgcgccggc gatgccgacc acccgtgcca gctgcggacc gatgttcttg accacgactt
     4321 cctgagagcc tgtcagttcg tcgacctgaa ttgccacgcg ttgggcgccg ctgtgcagca
     4381 gcagcaccca gtagaacttg cgggcctcgg ccaatgcacc ggtgtcgccg agcaggtgcg
     4441 gcaggaaatg gtacgggtag tcggcgccct gccagctgac cttgccggca tcgcgcatct
     4501 cggtcatgac gccgaccttg acctccatga cctggtcgat catcgatgcc ggaatcgcga
     4561 aagtgcggct gccgatgcgg accagcgccg cgttggttac tgccagcgtc agcggcaggt
     4621 agatgcggaa ggtcgttccc ttgccggtgt cgctctggac ttcgatgcgg ccgccgagat
     4681 cgccgacctc tgaacgcacg acgtccatcc cgacgccgcg accggatacc tgggtgacct
     4741 tggttgccgt cgaaaaaccc gagtggaaga tgaagtcggt gagtttgcgc ggctcgacct
     4801 cctgctgtgg accgatcagg cccttggcaa tcgccttctc gcggatgcgc tcaaggtcga
     4861 ggccgctgcc gtcgtcctgc atggtgatga tgacttcgtt gccttcctgg cgaagcgtca
     4921 gcgtgatctc gccgatatcg gccttgcccg ccgcgacgcg gcggtcaggc gtttcgatgc
     4981 catgcgacac ggcattgcgc agcaggtgct cgagcggacc gatcatcttg ttgatgatgt
     5041 tctggtcgag ttcgatctgg ctgccatgga cctcgaggtc agcgcgtttg ccggtttcct
     5101 tggcggtctg gcgggtgatc cggtagagcc gttcgactat gccggcgaag gggaccatcc
     5161 gcaccgacat cagcgcctgt tgcagctcgc ggttgagccg gccctggttg gcgagcgccg
     5221 cgttcgattc gtcgaggctc ttgagcagcg attgctggac agtcgccacg tcgttgaccg
     5281 actcggccat catccgcgtc agttcctgga aacgcgtgaa gcgatcgagt tccagcgggt
     5341 cgaaggaggc ggcggtttca ctggtcagcg cgatccgcga ctgcagctgc gattcagcct
     5401 ggatttcgat ctcgcgcaac tgccggcgca gacggatgac gttctcggtc aggtcgagca
     5461 tcgcctgttt gaggccgcgc atctcgccct cgatgcgggc gcgggcaatc gacagctcgc
     5521 cggcgtcgtt gaccagtcgg tcgatcagtt cggcgcgaac ccgcagcatc ggccgcgcca
     5581 gcgcatcgcc ctgcgcacgc tgcggagcga tcggcgcggc ggcgctcggg gcgatgccgg
     5641 ccgtgtcgtc ggcggcagac gagattggcg tgactgtcgc ctcgtgcggc gcagctgacg
     5701 gcgcttcggt ctgcgctttc ggttccggcg ttgtggcgac agcgggtggt tcggcagccg
     5761 cgtcgccggg tgcgtcgacg gtgatctcgg aaagtgcctc gccctgttgc aggcgttcga
     5821 tgacgccggc gatggtgtcg aagccaccct ggacgtcgaa gatcgcaccg gtatcagcct
     5881 ggccggactt tgtcagctcc tcaacgcgtg cctcgagcgt gtgggtcagc gcgccgatcg
     5941 acatcgcgcc ggccatgcgc gcgctgccct taagggtgtg tagggcgcga gccagcgcct
     6001 gctgggcgcc ctcgtcggcg ggcgcggcac gccaagcgcc gagctgggcg taaatcgtct
     6061 tgaccagttc ggtcgactct tcgaggaaga cgggcagcag gtcctcgtcc aggctgtcag
     6121 cgggcaacgg tacgctggtg gccgaccgcg cgggtgtcag gggcggattg atcgccgccg
     6181 acagtggcgg ttcggggatt agcggtggcg cctccggtgc cgtgatgtcg accgccgatg
     6241 gtgtcgtctc cgccggcggc ggttcggccg cggctatctc ggactcggcg atttcaatcg
     6301 tcggcagggc gttgtcgggc gcggccgact caggccgcag cgcggtaatc cggtcggcga
     6361 tttcgtcgaa ctcgagctgg aagcgggcaa tctcctcggg gtcggcagag ccgcggtggt
     6421 gcgcggcctc gaccatggtt tcgagcgtat gcgtcagcgc cccgatgccg agtgcgccgg
     6481 ccatgcgtgc gctgcccttg agcgtatgca agaggcgcgc aagggcgtgg atcgtgttga
     6541 cgtcgtcggg cttggcccgc cacatgccca gccgctcgtt gacggcgccg acgaggtcgc
     6601 cggcctcctc gatgaagatc ggcagtagcg attcgtcgag ttcgtcgggt gggagttcta
     6661 ctggcccggc actttcgggc gcttcgggct ggatgacgct ggtcgcggcg agcaggtcgg
     6721 cctcggcgtt ctcggccggt tcggcgtcgg catagacgct ggcgatgccg gcgaccagca
     6781 gcggccggtt ctcgggggtc tggcccgagg cgatcgtgac gaccattgac tcgagttcgg
     6841 cgatgacctc gcggatgact tccagggctt cgagattggc cgggccgcga acgtgggagc
     6901 gacgcagcaa ggctgattcg aggccgtgcc cgagttcctt gaccaccgcg aagccgacgg
     6961 tgccgccgat gctgcccagc gtatgggccg agcggatcat gtcgggcggc gccggcatcg
     7021 tcgggtcgag ttcgatctcg ccgaaggccc tcgtcagcgt ttcaagatgg cctcgcgctt
     7081 cttcgaggaa aatctgaaac agcaccggga ccgattcgtc atggtcatgg gtatcggcag
     7141 tcgggacggc gtcgatcgcc ctgatcggct ggtccgcgac cgtgggtacg gcaagctcgt
     7201 ccaacgagat gtcgtcagcc gccagctctg ggctctggct ggattccgct aactcaggcg
     7261 cctcgacgtc ggcctcgggt gcgggaaggt caagcgtaat ttcgggaagc ccggtttcgg
     7321 caaccagcgg ctcgtcgctc tctggtgcca attccggcgg cggggcgggc aaatcaccga
     7381 gatctatttc ggtgctggcg tcggcggcgg gatatggggc gggcaaggag tcgatggatt
     7441 cggctacttc accgaggtcg atctgcagat cggtttcggc gtcatcgaag ctgatcgtca
     7501 gcgcctcgtc ggtggcgggc tccgtttcgg catcgaccgg cgccagatcc agcgtcagct
     7561 cggaatcggg gttctccggc gcctcgccga ggtcgatcgt ctcttcggca gcggggggct
     7621 cttcgacgac cgcgggcgcc gtcggcgccg ccttctgcgc cagcgtaacc gtctccccac
     7681 ccttaagcga ttcggccatc gcgaccaacg cagccgggtc gggaagggag cccgcgccac
     7741 tggccagatg cgtgacccac gccgaaaaga cctcgtgcga aagctgcacg agcttgaaga
     7801 aattcaggtc gggcgtgcgc tcttggcgca accagaggtt attgacctgc tcgacaaccc
     7861 aagcgacttc accgaagtcg cgcaagccga ccatacggct gctgcccttg agcgtgtgga
     7921 aggcgcgccg catcgtcgtc agcgcgtcga cgttgctcgg ctgcgccttg agcaggtcga
     7981 ggttctcggc gatcgtgccg agcacctcat tggcctcctc gaggaagatc tccagcaatt
     8041 cggcgtcgat ctcggcgtca ctggcgctgt cttccggcgt ggccggcgcg acaggttccg
     8101 ttgtggtcgg cggcggcgcg ctaacgctgg cgctgatcgc cggcgcctcg accgttggcg
     8161 gcgaagcagg ctgctcgggg gcttccgcct tgggtggtgg cgaggtgagc gcagccgcct
     8221 tagcttctgc cgacagcgtt tgggtcgcga tcgcctcggc ttcctcaacg ctggcggcga
     8281 agccttcacc agatacggct gacggcgcag ccgagcgttg tccgagcgcg gcgacgaagc
     8341 tctgatagtc tgggttgcca ctgcgcagcg cctcgacgaa gaaacccagc gccgagagct
     8401 ggttggcgac atgctcgaaa tcggccgctt gcgcctgata tcccggcgcg gcgaaggcgg
     8461 cgatgcgttc gctggcatgc gtcagcgccg acacggcgtc gctttgtccc aacaccgtca
     8521 gcgcgccggc aatctgcgcc accggacgtt ggcaaccctt gagctgatcg cgttgctcgg
     8581 ggtcgcggaa gaatgtgtcg agggtctgtt cgatctgccc gaggttgctc tggatctcgc
     8641 ggacaacctg cgcgatcagc aacttctcct gcgccagacg ggtcatctcg tcgagcatcg
     8701 gaatctcggc gccggtggtc ggcggggtgc cgccgataca ggcatggagg cgggcgacca
     8761 tgacgtcgac ctggtgcacg aaatcggcgc caaggtgggc gaaattgtcg agcgtgttcg
     8821 agagcagcag gatggcggtc gccgtttcca tcgcgaccgc gtcgttatgg cgggacttgt
     8881 cctcggcgac ccacttagta atcgtcgcca gcgcctgagt caggcgcttg aaatcggtga
     8941 gcccgacctg ctcggcgccc ttgctgagcg ccgcggcggc ctcgtgagcg ggaccgatcg
     9001 cggtggagtt gccgccacag aagcggctcc agtt
//
LOCUS       DATDAA010000241         3772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_367928,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000241 DATDAA010000000
VERSION     DATDAA010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367928"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VIS73_07700"
     CDS             complement(<1..1126)
                     /locus_tag="VIS73_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY5763076.1"
                     /translation="MAGTFEKPEIVEEELDILLIGGGMACCGTAYEMMRWAEVVKKET
                     GKDLKIKLVDKAAMDRSGAVAQGLSAINTYIGPRQDPADYARMVSNDLMGITRDDLAY
                     DLGRNVDDSVHLFEEWGLPIWKTDSEGVRHDGAESIREGLPTLKDGGEPVRSGKWQIM
                     INGESYKWIVAEAAKKALGLDRIQERVFIVKLVNDKNDPNRIAGAVGFSTRENKIYVY
                     KAKAVMLAAGGCVNLFRPRSVGEGTGRAWYPVWNAGSTYAMAAEAGAELTMMENRFVP
                     ARFKDGYGPVGAWFLLFKAKATNAYGEDYMVKNKAMLDDYPPYGQAAVPASCLRNHLM
                     LKEMKEGRGPIYMDTVTALAKLAETLTPKEVKHLEAEAWED"
     gene            complement(1155..1628)
                     /gene="aprB"
                     /locus_tag="VIS73_07705"
     CDS             complement(1155..1628)
                     /gene="aprB"
                     /locus_tag="VIS73_07705"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA];
                     GO_process: GO:0019424 - sulfide oxidation, using siroheme
                     sulfite reductase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit beta"
                     /protein_id="HEY5763077.1"
                     /translation="MPTFVRTDKCDGCKGQDKTACMYICPHDLMKLDKDGSETGHAMK
                     AFNQEPEQCWECYSCVKICPQQAIEARHYADIVPLGGSVQPLRGSDSIMWTIKFRNGT
                     LKRFKFPIRTTPEGSIDPYGGKPMPTMADLTAEGVFTRSVMGGFRAGNPAELIRK"
     gene            complement(1684..2895)
                     /gene="sat"
                     /locus_tag="VIS73_07710"
     CDS             complement(1684..2895)
                     /gene="sat"
                     /locus_tag="VIS73_07710"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEY5763078.1"
                     /translation="MSRLVKPHGGGELKPLLLEGDAARAEMERAKFLPKVKVSSREKG
                     DIIMLGIGGFTPLEGFMSHADWQGVCDGMRMANGLFWPIPVTLSTDQATAEGIKTGVE
                     VALVDGESGDILATMKVSEKYAIDKSHECMTVYKTTELEHPGVKMVMEQGEINLAGPI
                     KVLSQGGFAEKYKGLFHTPAQTRAIFESFGWAKVAAFQTRNPMHRSHEYLAKVAIEVC
                     DGVLIHSLLGNLKPGDIPADVRTQAIGTLIHRYFVEKTVVQSGYPLDMRYAGPREALL
                     HALFRQNYGCSHLIVGRDHAGVGSYYGPFDAHHIFDEIPKDALETQALKIDWTFWCYE
                     CGGMASARTCPHDEAKRLLLSGTKLRKMLSEDAEVPAEFSRPEVLAILRKYYAGLEEK
                     VEIKLAGHSAR"
     gene            complement(2972..3442)
                     /locus_tag="VIS73_07715"
     CDS             complement(2972..3442)
                     /locus_tag="VIS73_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEY5763079.1"
                     /translation="MEGKKMAIIATKGTLDWAYPPLILASTAATLGYEVQVFFTFYGL
                     ALLKKDMGDIKISPLANPAMPMPIPMPVFVQALPGMEAMATMMMKQKMKAKGVASLET
                     LRETCQELDVKFIACQMTVDLFEFDKSDFIDNIEFGGAATFLEFAGDADITLYI"
     gene            complement(3482..3709)
                     /locus_tag="VIS73_07720"
     CDS             complement(3482..3709)
                     /locus_tag="VIS73_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY5763080.1"
                     /translation="MDFDKELDARGLSCPLPIVKTKKSLNDMQSGQVLKVVSTDPGSI
                     KDMQAFANQTGNPLLESAEASGAYTFYLRKK"
BASE COUNT          612 a         1175 c         1161 g          824 t
ORIGIN      
        1 agtcttccca ggcttccgcc tcgaggtgct tcacttcctt cggcgtcagc gtctcggcca
       61 gcttggcgag cgcggtgacg gtgtccatgt agatcgggcc gcgaccttcc ttcatctcct
      121 tgagcatcag gtggttgcgc aggcacgaag ccggcacggc agcctggccg tagggcgggt
      181 agtcgtcgag catggccttg ttcttgacca tgtaatcctc accgtaggcg ttggtggcct
      241 tggccttgaa cagcaggaac caggcaccga cagggccgta gccgtccttg aagcgggcgg
      301 gaacgaagcg gttttccatc atcgtcagct cggcgccagc ttcggcggcc atcgcgtagg
      361 tcgagccagc gttccacacc gggtaccagg cgcggcccgt gccttcgccc accgaacgcg
      421 gacggaacag gttcacgcag ccgccggcgg cgagcatgac agccttcgcc ttgtagacgt
      481 agatcttgtt ctcgcgggtc gagaagccga cggcaccggc gatgcggttg gggtcgttct
      541 tgtcgttgac cagcttgacg atgaacacac gttcctggat gcgatcaagg ccaagcgcct
      601 tcttcgcagc ttcggcgacg atccacttgt aggactcgcc attaatcatg atctgccact
      661 tgcccgaacg aaccggctcg ccaccgtcct tgagcgtcgg caggccttcg cggatggatt
      721 cggcgccgtc gtggcggacg ccttccgagt cggttttcca gatcggcagg ccccactcct
      781 caaacaggtg caccgaatca tcgacgttgc ggccgagatc gtaggcgaga tcgtcgcgtg
      841 tgatgcccat caggtcgtta gagaccatgc gcgcgtaatc ggcggggtcc tgacgcggcc
      901 cgatataggt gttgatcgcc gacagaccct gggcgacagc gcccgagcgg tccatcgcgg
      961 ccttgtcgac cagcttgatc ttcagatcct tgccggtttc cttcttcacc acttcggccc
     1021 agcgcatcat ctcgtacgcg gtgccgcagc aggccatgcc gccgccgatc agcaggatgt
     1081 cgagttcttc ttcgacgatt tccggttttt cgaatgttcc agccattttc tttccctatc
     1141 ctcgattggt ctgattactt gcggatcagc tcggccggat ttccggcgcg gaagccaccc
     1201 atgacgctgc gcgtgaacac gccttcggcg gtcagatcgg ccatcgtcgg catcggcttg
     1261 ccaccgtagg gatcgatcga gccctcgggc gtggtgcgga tcgggaattt gaagcgcttg
     1321 agcgtgccgt tgcggaactt gatcgtccac atgatcgagt ccgagccgcg cagcggctgc
     1381 accgaacctc cgagcggcac gatgtcggcg tagtgacgtg cctcgatggc ctgctgcgga
     1441 cagatcttga cgcaggagta gcactcccag cactgctcgg gttcctggtt gaaggccttc
     1501 atcgcatggc cggtttcgga accgtccttg tcgagcttca tcaggtcgtg cggacagatg
     1561 tacatgcagg ccgtcttgtc ctggcccttg catccgtcgc acttgtcggt acgaacaaaa
     1621 gttggcattg cgttacctcc ttgcggtttt tgatccccgc gcgcgacgcg caggactttg
     1681 aattcagcgc gccgaatggc ccgccagctt tatttcgacc ttttcttcga gcccggcgta
     1741 gtacttgcgc aggattgcca gtacttcggg ccgcgagaac tcggccggca cttcggcgtc
     1801 ttcggaaagc atctttctga gcttggtgcc cgagagcagc aggcgcttcg cttcgtcgtg
     1861 ggggcaggtg cgcgccgatg ccatgccgcc gcactcgtag caccagaagg tccagtcgat
     1921 cttcagcgcc tgcgtttcga gcgcgtcctt cgggatctcg tcgaagatgt ggtgggcgtc
     1981 gaagggcccg tagtagctgc cgacgccggc gtggtcgcgg ccgacgatca ggtgcgagca
     2041 gccgtagttc tggcggaaca acgcgtgtag caaggcctcg cgcggtccgg cgtagcgcat
     2101 gtcgagcgga tagccggact ggaccacggt cttctcgacg aagtagcggt ggatcagcgt
     2161 gccgattgcc tgcgtacgca cgtcggccgg aatgtcgccc ggcttgaggt tgccgagcag
     2221 cgagtggatc aatacgccgt cgcaaacctc gatggcgacc ttcgccaggt attcgtgcga
     2281 gcggtgcatc gggttgcgcg tctggaaggc ggcgaccttg gcccagccga aggattcgaa
     2341 aatggcgcgc gtctgcgccg gggtatggaa cagccccttg tacttctcgg caaagccacc
     2401 ctgcgacagc accttgatcg gacccgcgag gttgatctcg ccctgctcca tgaccatctt
     2461 gacgccgggg tgctcgagct cggtggtctt gtagaccgtc atgcactcgt gcgacttgtc
     2521 gatcgcgtac ttttcgctga ccttcatcgt cgccaggatg tcgccactct cgccatcgac
     2581 cagcgcaact tcgacgccgg tcttgatgcc ctcggcagtc gcctggtcgg tggacagcgt
     2641 caccgggatc ggccagaaca ggccgttggc catacgcatg ccgtcgcaga cgccctgcca
     2701 gtcggcgtgg ctcatgaaac cctcgagcgg cgtgaaaccg ccgatgccga gcatgatgat
     2761 gtcgcccttc tcgcgcgaac tcaccttcac cttgggcagg aacttggcgc gctccatttc
     2821 ggcacgcgct gcatctcctt cgagcagcag cggtttgagc tcaccacctc catgcggttt
     2881 cactagtctc gacatgcgat tcctccctgt tcccctttgg tgccgttcgt ccggcaccgg
     2941 ctgcgccggc accgccgtct cttttgtctt gttagatgta cagcgtgatg tctgcgtcgc
     3001 cggcgaactc aaggaaagtc gccgcgccgc caaactcaat gttgtcgatg aaatcggact
     3061 tgtcgaactc gaagaggtcg accgtcatct ggcaggcgat gaacttgacg tccagctctt
     3121 ggcaggtctc gcgcagcgtc tccagcgagg cgacgccctt cgccttcatc ttctgtttca
     3181 tcatcatggt cgccatcgct tccatgcccg gcagcgcctg cacgaagacc ggcatcggaa
     3241 tcggcatcgg catggccggg ttggcgagcg ggctgatctt gatgtcgccc atgtccttct
     3301 tcaacagcgc aaggccgtag aaggtgaaga agacctgtac ctcgtagccg agcgtggcgg
     3361 ccgtcgatgc gaggatcaac ggcgggtagg cccagtcgag cgtgcccttg gtggcgatga
     3421 tcgccatctt cttgccttcc atccaagcct cccgcgtgtg tgagttgaga tcggcccgcg
     3481 cttacttctt gcgcaggtag aaggtgtagg cgccgctggc ttcggcagat tccagcaagg
     3541 gattgccggt ctggttggcg aaggcctgca tgtccttaat cgagcccgga tcggtcgaaa
     3601 ccaccttgag cacctggccg ctctgcatgt cgttgagcga cttcttggtc ttgacgatcg
     3661 gcagcgggca cgagaggccg cgggcatcga gttccttgtc gaaatccatg ttgttttctc
     3721 tccagtcttt atggtttgcg ttgtgattcg ttgcggatct gctcccgcct tc
//
LOCUS       DATDAA010000242         3494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_402527,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000242 DATDAA010000000
VERSION     DATDAA010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402527"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VIS73_07725"
     CDS             complement(<1..320)
                     /locus_tag="VIS73_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763081.1"
                     /translation="MHELLREIESITACRDRESLAAHLLTAVRALLGDGQVALLDIES
                     RRDPVRHRGYAEITADGVRFLRTDDALAGFALATPVALIERAVRGSEAVLDETDERFY
                     CVFPL"
     gene            complement(406..945)
                     /locus_tag="VIS73_07730"
     CDS             complement(406..945)
                     /locus_tag="VIS73_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEY5763082.1"
                     /translation="MRMRRDDSILLLIDIQEKLYPTISDGETLLAHCQWLADLAARLA
                     VPTILTEQYPAGLGPTLPALRDRCPAAPVIEKMHFSAVVGGNLLPACGSRRQFVVAGT
                     EAHVCVLQTVLDLIAPEREVFVVAEAVGSRRPEDRALAIERMRQAGAVIVSREMVAFE
                     WLDRAGTDTFRTISREMIR"
     gene            1111..1779
                     /locus_tag="VIS73_07735"
     CDS             1111..1779
                     /locus_tag="VIS73_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEY5763083.1"
                     /translation="MVLNIDVVSDFVCPWCFVGKRHLLAALRQFGERNPGVATATRWL
                     PFFLNPDTPPEGEPYRPFLERKFGGSDAVDALQADVAAAGKGVGIRFAFDRIELRANT
                     LDAHRLIYRAQQPDSGIDASLLSERLFSAHFCEGKHIGSSDVLVRLAVNAGDREADVR
                     RFIESGEGTREVVDIAAQIQQLGIGGVPFFIFNRQVAISGAQHDGALLEAMEKAIVES
                     GGEA"
     gene            1806..2636
                     /locus_tag="VIS73_07740"
     CDS             1806..2636
                     /locus_tag="VIS73_07740"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY5763084.1"
                     /translation="MATVTLASGLLGPGMATATEMAVPPQTGPAAQADFSSYLAAMPG
                     KAFVVGPGGAWAWQSGQVSGEAALGLALAACNAQAQIKCVPYAVDDRRVFDEGAWAKA
                     WGPYLSRRDAADRPVGLMRGARFPDIEFVDAKGKQRALAQFRGRAVVLHFWGSWCAPC
                     RHELPDMARFAKAMAGHEIAFLPLQVRESYADSQRWLAREQIALTVFDSGMRSADDGE
                     FRVRGGGRLPDRAVAPVFPSTVFLDRHGVIVFVHHGPIARWEDYRPQLLNLLAGQRKR
                     "
     gene            complement(2610..>3494)
                     /locus_tag="VIS73_07745"
     CDS             complement(2610..>3494)
                     /locus_tag="VIS73_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5763085.1"
                     /translation="PGPMNARDLGRRALTVLIILAVPLLMIRLFQDSLLYYPERASRD
                     DYLRIAAGAGLSAWPDADNLRGLVADPPAGLDAAVAGTVAVLHGNAGNAAYRDYYVPV
                     LTQLGYRVVLLEYPGYGSRTGKVGEASLVADAVESLRLLHRQSPLPLILIGESLGAGV
                     AAATAGQAPDALDALMLITPWDKLASVAQHHYPWAPVRLMLRDRYDSAVNLRAFPKPI
                     TLVVAEHDEIIPPVFARQLFEAIGTAGGRNTLVTIGGAAHNDWAWRIDKQWWRSQLAA
                     LRGAKDASNPLALPLAGE"
BASE COUNT          557 a         1126 c         1223 g          588 t
ORIGIN      
        1 agcgggaaga cacagtagaa acgctcgtca gtctcgtcga gaaccgcttc gctaccgcga
       61 acggcccgtt cgatcaatgc caccggcgtg gccagcgcga agccggccag cgcatcgtcg
      121 gtccgcagga aacggacacc atcggcggtg atttcggcat agccgcgatg gcgaacgggg
      181 tcacgacgcg actcgatatc gaggagcgcg acctgaccgt cgccgagcag cgcccggact
      241 gctgtcagca ggtgcgctgc cagactctcg cggtcgcggc aggcagtaat cgattcgatt
      301 tcgcgaagca actcgtgcat tgaaacgccc tatgacgata ggcctaacgc aaagcgcgtc
      361 atggtaacgg agcaacattg ccaacgggca agcccctgca ccttactagc ggatcatctc
      421 gcggctgatg gtgcgaaagg tatcggttcc ggcgcggtcc agccactcga aggcgaccat
      481 ctcgcgcgag acgatcacgg cgccggcctg gcgcatgcgc tcgatggcca gcgcgcggtc
      541 ctcgggccgt cgcgagccga cggcctcggc gacgacgaag acctctcgtt cgggcgcgat
      601 taggtcaagc acggtctgca ggacgcagac atgggcttcg gtgccggcga ccacgaactg
      661 ccggcggctg ccgcaggccg gaaggagatt tccgccgacg acggccgaaa agtgcatctt
      721 ttcgatcacc ggggccgccg ggcagcgatc acgcagggcg ggcagcgttg gcccgagtcc
      781 ggccgggtac tgctcggtga gtattgtcgg taccgcgagc cgcgcggcga gatcggcgag
      841 ccactggcag tgggcgagca gggtctcgcc gtcgctgatc gtcgggtaga gcttctcctg
      901 gatgtcgatc agcaggagga tcgagtcgtc gcgccgcatg cgcatgatgc ttgccccgtt
      961 gtctggtgcc gaaacgccat tatcgccccc gccgctgcca cccactcgat taccagcgcc
     1021 tgcggcgcct tgccggcgct gcggatgggc gtggcgttat tatcggctat cccggcgcgt
     1081 cctgccggcc cacggcgaaa ggccccgaag atggtcctca acatcgacgt cgtttccgac
     1141 ttcgtctgtc cctggtgctt cgtcggcaag cgccatctgc tagccgcgct gcgccagttc
     1201 ggcgagcgca atcccggtgt cgcgacggcg acgcgctggc tgccgttctt cctcaatccc
     1261 gatacgccgc ccgagggcga gccctaccgg ccctttctcg aacgcaagtt cggcggcagc
     1321 gacgcggtcg atgcgctgca ggccgatgtc gctgccgccg gcaagggcgt tggtattcgc
     1381 tttgcctttg atcgcatcga actgcgcgct aacacgctgg acgcgcaccg gctgatctac
     1441 cgggcgcagc agccggattc gggaatcgac gcgagcctgc tttccgagcg tctgttctcg
     1501 gcgcacttct gcgagggcaa gcacatcggc tcaagtgacg tgctggttcg gctggcagtc
     1561 aatgccggcg accgcgaggc cgatgtgcgg cgtttcatcg aatcgggcga gggtacccgc
     1621 gaggtcgtcg acatcgccgc acagattcag cagctgggca ttggcggcgt accgttcttc
     1681 atcttcaacc gccaggtcgc gatcagcggc gcccagcacg acggggcgct gctcgaagcg
     1741 atggagaagg ccatcgtcga gtccggaggt gaagcctgat ggggttgtgc aggcgattta
     1801 tcggcgtggc gaccgtgacg ctcgccagcg gcctgctggg gccgggaatg gccactgcca
     1861 ccgagatggc ggtgccaccg caaaccggcc cggcggcgca ggctgatttc tccagctatc
     1921 ttgcggcgat gccgggtaag gccttcgtcg taggccctgg cggcgcctgg gcctggcaga
     1981 gcggtcaggt gagcggcgag gcagctctcg gtctcgcgct cgcggcctgc aacgcgcagg
     2041 cacagatcaa gtgcgttccg tatgccgtcg acgaccgccg cgtcttcgac gagggtgcct
     2101 gggccaaggc ctggggcccg taccttagcc gccgtgatgc cgccgaccgg ccggtcggcc
     2161 tgatgcgcgg cgcgcgtttt cccgatatcg aattcgtcga cgccaagggc aagcaacgcg
     2221 cgctcgcgca gtttcgcggc cgcgccgtcg tcctgcattt ctggggatcg tggtgtgcgc
     2281 cgtgccgcca tgaactgccc gacatggcac gtttcgccaa ggcgatggcc gggcatgaga
     2341 tcgccttcct accgctccag gtgcgtgagt cgtacgccga ttcgcagcgc tggctggcgc
     2401 gcgagcagat cgcgctgaca gtctttgatt ccgggatgcg ctcggccgac gacggcgagt
     2461 tccgcgtgcg gggcggcggg cgcctgcccg atcgtgccgt ggcgccggtc tttcccagca
     2521 cggtctttct cgatcggcat ggcgtgattg tctttgtgca ccacgggccg atcgcccgct
     2581 gggaagatta tcgtccgcag ctgcttaacc tactcgccgg ccagcggaag cgctagcgga
     2641 ttgctggcat ccttggcgcc gcgcagcgcc gcgagctgcg aacgccacca ttgcttatcg
     2701 atgcgccagg cccagtcgtt gtgcgcagcg ccaccgatgg tcaccagtgt gttccgtccg
     2761 ccggctgtgc cgatggcctc aaagagctgt cgggcgaaca cgggcggaat gatctcgtcg
     2821 tgctcggcga cgaccagcgt gatcggcttc gggaatgcgc gcaggttgac ggcgctgtcg
     2881 tagcggtcgc gcagcatcag gcgtaccggt gcccagggat agtggtgctg cgcgaccgac
     2941 gccagcttgt cccagggggt gatcagcatc agcgcgtcga gtgcatcggg cgcctgtccg
     3001 gcagtggcgg cagcgacgcc ggcgccgagc gattcgccga tcaggatcag gggcagcggc
     3061 gactggcggt gcagaagtcg cagcgattcg accgcatcgg cgaccagcga cgcctcgccg
     3121 accttgccgg tacgagatcc atagcccgga tactcgagga gcacgacgcg gtaaccgagt
     3181 tgggttagca ccggtacgta gtaatcgcgg taggccgcgt tgccggcatt gccgtggagg
     3241 acggcgaccg tgccggccac cgcggcgtcg aggcccgccg gcgggtcggc gaccagtccg
     3301 cgcagattgt cggcatcggg ccaggcagac agcccggcgc cggcggcgat ccgcaggtag
     3361 tcgtcgcggc tggcgcgctc ggggtagtag agcagcgagt cctggaacag gcgaatcatg
     3421 agcagtggca ccgcaaggat gatgaggacg gtgagtgcgc ggcgaccgag atcgcgggcg
     3481 ttcatcggac cggg
//
LOCUS       DATDAA010000243         2258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_52535,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000243 DATDAA010000000
VERSION     DATDAA010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52535"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VIS73_07750"
     CDS             complement(<1..608)
                     /locus_tag="VIS73_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEY5763086.1"
                     /translation="MSHDRGADVYLENRTFDEIAVGDFSILHRVLTREDIKLFAIMSG
                     DVNPAVVDPEFAESGMFREVVAHGMWSAALISTMLGTEFPGPGTILIDQSLHFDRPVT
                     IGDSIAVKVTVKEKYDHNKHMTLDCVCTNQEGLQVVHGHLEVLAPTEKIRRKAMNLPQ
                     ITISDKEARYQHLLGMAAGLEPIDVAVVHPCDAESLRGPVMAA"
     gene            complement(639..1406)
                     /gene="fabI"
                     /locus_tag="VIS73_07755"
     CDS             complement(639..1406)
                     /gene="fabI"
                     /locus_tag="VIS73_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY5763087.1"
                     /translation="MFNLNGKRGLVVGIANNKSIAYGCAKAFKLCGAELAVTYFNEKA
                     EPHVRPLAELLGAQLVLPLDVEQEGQMESVFEAIDKQWGNLDFVVHSIAFAPKDDLHG
                     RVTDSSREGFMRAMDISCHSFIRMAKLAEPLMKNDGCLLTMSYLGADEVIENYGIMGP
                     VKAALQASSRYLASELGPRGIRVHAISPGPLHTRAASGIPHFDELMKDAIDRAPLHKL
                     VSIEDIGALSAYLVSDVARTLTGGTIYIDGGYHIVGS"
     gene            1663..>2258
                     /locus_tag="VIS73_07760"
     CDS             1663..>2258
                     /locus_tag="VIS73_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY5763088.1"
                     /translation="MESTDQALDRALVPILQRYQNDPTCVVQILRELQAACSYLSPRA
                     ISEVAKALNLPRAHVEGVAGFYSFLHLEPAGEYRILFSDNITDRMLGNLSLVDRLCAK
                     LWLERGRVSEDGLVSVDLTSCTGMCDQGPAMLVNNRAITRASPERIDHIAELVRARTP
                     VEQWPAEYFEVVDNICRRDVLLDSPLKPGEALRAAVARG"
BASE COUNT          389 a          697 c          727 g          445 t
ORIGIN      
        1 gcggccatga ccgggccgcg cagcgactcg gcgtcgcagg ggtggaccac tgccacgtcg
       61 atcggctcaa gtccggcggc catgcccagc aggtgctggt agcgcgcctc tttgtcgctg
      121 atggtgatct gcggcaggtt catcgccttg cgccggatct tctcggtcgg cgcgaggact
      181 tccagatggc catgaacgac ctgcaggccc tcctggttgg tgcatacgca gtcgagcgtc
      241 atgtgcttgt tgtggtcgta cttctccttg actgtcactt ttaccgcgat cgagtcgccg
      301 atggtgaccg gtcggtcgaa gtgcaggctc tggtcgatga ggatggtccc cggccccgga
      361 aactcggtgc cgagcatcgt cgagatcagc gccgcgctcc acatgccgtg ggcgaccacc
      421 tcgcggaaca taccgctttc cgcgaactcg gggtcgacga cggccggatt gacatcgccc
      481 gacatgatgg cgaagagctt gatgtcctcg cgcgtcagca cgcggtgcag gatcgagaaa
      541 tcgccgacgg cgatttcatc gaaggtgcgg ttctcaagat agacgtcagc tccgcgatcg
      601 tggctcatgt gaactcctgg tttcgtttgg cgcgcagttt aactgccgac gatgtggtac
      661 ccaccgtcga tgtagatcgt gccgccggtc agcgtgcggg cgacgtcgct gaccagatag
      721 gcggaaaggg cgccgatgtc ctcgatcgac accagcttgt gcaacggcgc gcggtcgatt
      781 gcgtccttca tcaattcgtc gaagtgtggg atgcccgagg cggcgcgcgt gtgcagcggt
      841 cccggcgaaa tcgcatggac gcgaatgccc ctggggccga gttcggaggc cagataacgc
      901 gacgaagcct gcagcgcagc cttcaccgga cccatgatgc cgtagttctc gatcacttcg
      961 tcggcgccca ggtagctcat ggtcagcagg cagccgtcgt tcttcatcag gggctcggcg
     1021 agcttggcca tgcgaatgaa cgagtggcag gagatgtcca tcgcgcgcat gaagccctcg
     1081 cggcttgagt cggtgacccg gccgtgcagg tcatccttgg gcgcaaacgc gatcgagtgc
     1141 acgacgaaat caaggttgcc ccactgcttg tcgatggcct cgaatacgga ctccatctgg
     1201 ccttcctgct cgacgtcgag cggaaggacc agttgcgcgc cgagcagttc ggcaagcggg
     1261 cgaacgtgcg gctccgcctt ctcgttgaaa taggtgacgg cgagttcggc gccgcagagc
     1321 ttgaatgcct tggcacagcc gtaggcgatg ctcttgttgt tggcgatgcc gacgacgagg
     1381 ccccgtttgc cattgaggtt gaacatgatg attctccgga ttggggttgg tggtcgctca
     1441 cggcttggcg caaaaatggt gcggcgcagg atatgtaagc gttctgtttt gtaaaggtta
     1501 tcactttatt gaagcgactg tctgctgcga acgcaaaaga cgtgaacggc ttctcggctg
     1561 gccgttggcg cgggccccaa acttgatcga ggtcactgta gcgccatgct gcactgcgta
     1621 aaatcaccga ctttcgtctg accgcaagcc aaaagggaga ccatggagtc caccgatcaa
     1681 gcgctcgacc gtgccttggt gccgatcctg cagcgatacc agaacgatcc gacctgcgtc
     1741 gtccagatcc tgcgggagct gcaggctgcc tgcagctatc tgtcgcctcg ggcgatcagc
     1801 gaagtcgcca aggcgctaaa tctcccgcgc gcccacgtcg aaggcgttgc cggcttctat
     1861 tccttcctgc acctcgagcc ggccggcgag taccgcatcc tcttctccga caacatcacc
     1921 gaccgcatgc tcggcaactt gtcgctggtc gaccggctct gcgccaagct ctggctcgag
     1981 cgtggccggg tctccgagga tgggctggtc agcgtcgatc tcacctcgtg caccggcatg
     2041 tgcgaccagg ggccggcgat gctggtcaac aatcgggcga tcacccgggc ctcgcccgag
     2101 cgtatcgacc acatcgccga actggtgcgg gcgcgcacgc cggtcgagca atggccggcc
     2161 gagtatttcg aggtcgtcga caacatctgt cgccgcgacg tcctgctcga ctcgccgctc
     2221 aagcccggcg aggcgctgcg cgccgctgtc gcgcgcgg
//
LOCUS       DATDAA010000244         5690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_157696,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000244 DATDAA010000000
VERSION     DATDAA010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157696"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="VIS73_07765"
     CDS             complement(<1..1144)
                     /locus_tag="VIS73_07765"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY5763089.1"
                     /translation="MDTLTRGRPYPLGAHWDGAGVNFAVFSEHARVVELGVERAGAWR
                     FRALPACTDGVWHGYLPQAAPGTRYGFRAQGPNTPAAGHRFDGARLLLDPAARGLVGR
                     FDWHRLGDAESAGGLANLVIDDRFDWRGDVAPAAPLADSLLYEIHVRGATMTHPEVPP
                     ELRGRYGGLAAPAMLEHYRRLGVTALNLLPVQAFIDEKRLIESGLRNYWGYNPISFSV
                     PEARYAAADGQAAVDEFKSMVRALHAEGIEVILDVVFNHTAETDEEGPVLSFRGLDNA
                     VWYRLDPRDRSLYENFSGCGNTVNLAQPRVLQVVMDALRYWVGEMHVDGFRFDLAASL
                     TRDSAFLDAVAQDPLLARAKLIAEPWDLGPDGYRLGRFPAGWSEWND"
     gene            complement(1132..3312)
                     /gene="glgB"
                     /locus_tag="VIS73_07770"
     CDS             complement(1132..3312)
                     /gene="glgB"
                     /locus_tag="VIS73_07770"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY5763090.1"
                     /translation="MTTLARSEVEALAGGACGDPFAALGMHAGDDGHPWVRCLLPMAR
                     EVAIVDAASGKLVGSLGQTHPAGFFEGALTRRKRCFDYRLRVDWGEGPVEIDDPYRVP
                     PVLGEMDVWLLAEGSHRRPFERLGAHRCVIDGVGGTAFAVWAPSAQRVSVVGDFNHWN
                     GIVHPMRLRRECGVWEIFLPGVSAGARYKFEIRGADGTVLPLKADPYGIAAELRPSTA
                     SVVADLPPAVAPERIPAGERLNAPVSIYEVHLASWRRKADGSFLDWQSLAASLIPYVV
                     ELGFTHIELLPVSEHPFDGSWGYQPLGLYAPTARHGSASGFRTFVDSCHKAGLKVIAD
                     WVPAHFPSDEHGLGRFDGTPLFEHADPREGFHPDWGTLIYNFGRREVCNYLVGNALYW
                     IERFGIDGLRVDAVASMLYRDYARREGDWIPNSFGGRENLEAVAFLRRLSAVLGEEAP
                     GAATFAEESTAWPGVTRPPAMGGLGFHYKWNLGWMHDVLGYFSQDPVHRRHHHNQLSF
                     GLMYAFSENFVLPLSHDEVVHGKGSLIGRMAGDRWQKFANLRALYGFMWAHPGKKLLF
                     MGGEFGQWREWNHDAQLDWHLLDEPEHAGVQRLVGDLNRLYHQSPALYESDFDPAGFE
                     WLVVDDAAHSVFAFVRRARDLRRTIVCVGNFTPVVRHGYRIGVPGPGAYRERINTDSR
                     HYAGSDVGTPLGAATAEAVRCGDREWSVVLTLPPLATVLYEWIP"
     gene            complement(3312..>5690)
                     /locus_tag="VIS73_07775"
     CDS             complement(3312..>5690)
                     /locus_tag="VIS73_07775"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEY5763091.1"
                     /translation="IASAPLTAGSDETQRAMAYMAREELAARWVATQSEDSAVKARRI
                     YYLSMEFLVGRALNNALDALNLRDPAAAALAGSGSDIDAVLATEPDAALGNGGLGRLA
                     ACFLDSMATLGLPSWGYGMRYEYGMFAQSVHNGCQVEHPDSWLENGTPWEFPRPEVHY
                     TIRFGGTVDHHGDWAEWHAADAVEAKAFDYVIPGHSTSRVSSLRLWKAAAPTHIDLGA
                     FNKGDYARAAEFKNHFENISWVLYPNDSTPAGRELRLRQEYFFVAASVQDIVARHLGE
                     HGTLDNLADKVAIHLNDTHPAIGVAELMRILVDDHAVAWADAWTSCRRIFSYTNHTLM
                     PEALETWPLTLIQHVLPRHMRIIYRINQEFLDEVARRFPGDSDLIRRVSLIDEGNGHE
                     HDKRVRMAHLCIVGSHRVNGVSKLHSELMVQTIFADFAKLDPAKFHNKTNGVTPRRWL
                     AQSNRDLSAAIDARLDPGWRIDLDRLSSLRTFAGDADFQAECVAAKLANKARLAEYIA
                     TTQGIHVDPASLFDVQVKRIHEYKRQLLNVLHVITRYNAMLANPDAEFAPRTVIFAGK
                     AASAYHMAKLIIRLIHDVASVVNKDPRTRDRLKVVFLPNYGVSLAEIIMPAADLSEQI
                     STAGTEASGTGNMKLALNGALTIGTEDGANIEIRDQVGADNIFIFGKSAAEVMEIRAN
                     GYDPYEIYRHNDALRTVLDQIDTGFFSPGEPGRYHDIFNALARYGDHYLLLADYADYI
                     STQARVDALYLEPAAWARTSILNIAGMGPFSADRTIREYAVDTWHIAPGRPR"
BASE COUNT         1011 a         1849 c         1928 g          902 t
ORIGIN      
        1 gatcgttcca ctcgctccag ccggcgggaa atcgtccgag ccggtagccg tccggaccga
       61 gatcccacgg ctcagcaatc agcttcgcgc gggcgagcaa ggggtcctgc gcaacggcgt
      121 cgagaaaggc cgaatcgcgc gtcagcgacg cggcgagatc gaaacggaag ccatcgacat
      181 gcatctcgcc gacccagtag cgcaaggcgt ccatgaccac ctgcagcacg cgcggttgcg
      241 cgagattgac ggtattgccg cagccgctga aattctcgta gagcgaccgg tcgcgcgggt
      301 cgagccggta ccagacggcg ttgtcgagac cgcgaaagga aaggaccggc ccttcttcgt
      361 cggtctcggc cgtatggttg aagacaacgt cgaggatgac ttcgatgcct tctgcgtgca
      421 gcgcccgcac catggacttg aactcgtcga cggcggcctg tccgtcggct gcggcgtagc
      481 gcgcctcggg caccgaaaag ctgatcgggt tgtagcccca gtagttgcgc agtcccgatt
      541 cgattaggcg cttctcgtcg atgaaggcct gcaccggcag caggttgagc gcggtcacgc
      601 cgagccgccg gtagtgctct agcatcgccg gcgcggcgag gccgccgtaa cgcccgcgca
      661 gttccggcgg cacctcgggg tgcgtcatcg tcgcgccgcg aacgtggatc tcgtagagca
      721 gcgaatcggc gagcggcgcc gccggcgcga cgtcgccacg ccagtcgaag cggtcgtcga
      781 tgacaaggtt ggccaacccg ccggcgctct cggcatcgcc cagacggtgc cagtcgaagc
      841 ggccgacgag tccgcgcgcc gccgggtcga gcagcaggcg cgcgccatcg aagcggtggc
      901 cggcggccgg cgtgttcgga ccctgcgcgc ggaatccgta gcgggtgccc ggcgcggcct
      961 gcggcaggta gccgtgccag acgccatcgg tgcaggccgg caaggcacgg aaacgccatg
     1021 cgccggcccg ttccacgccg agttcgacaa cgcgtgcgtg ttcggaaaaa accgcgaagt
     1081 tgaccccggc gccgtcccag tgcgcgccga gcggatacgg ccggccgcgg gtcagggtat
     1141 ccattcgtag agcacggtcg ccagcggcgg cagcgtcagc accaccgacc actcgcggtc
     1201 gccgcaacgc accgcttcgg ccgtcgccgc cccgagcggg gtaccgacat cgctgccggc
     1261 gtagtgccgg gaatcggtgt tgatgcgctc gcggtaggcg ccggggccgg gcacgccgat
     1321 gcggtagccg tggcggacta ccggcgtgaa gttgccgacg cagacgatgg tccgccgcag
     1381 gtcgcgggcg cgacgaacga aggcgaatac cgaatgcgcg gcgtcgtcga ccaccagcca
     1441 ctcgaagccg gccggatcga aatcgctttc gtagagcgcc gggctctggt ggtagaggcg
     1501 gttgagatcg ccgaccagac gctgaacgcc tgcgtgctcg ggctcgtcga gcagatgcca
     1561 gtcgagctgc gcgtcgtggt tccattcgcg ccactggccg aactctccac ccatgaacag
     1621 cagtttcttg cccggatgcg cccacatgaa gccgtagagc gcgcgcaggt tagcgaactt
     1681 ctgccagcgg tcgccggcca tcctgccgat cagcgagccc ttgccgtgca ccacctcgtc
     1741 gtgcgagagc ggcagcacga agttctcgga gaacgcgtac atcaggccga aggacagctg
     1801 gttgtggtgg tggcgccggt gcaccgggtc ctggctgaaa taaccgagta catcgtgcat
     1861 ccagccgagg ttccacttgt agtggaaacc cagcccgccc atcgccgggg gtcgcgtcac
     1921 gccgggccag gccgtcgatt cctcggcgaa ggtcgccgcg cccggagcct cctcgccgag
     1981 taccgcgctc agccggcgca ggaaggcgac ggcctcgagg ttctcacgac cgccgaagga
     2041 attgggaatc cagtcgccct cgcgccgcgc gtaatcgcgg tagagcatcg aggcgacagc
     2101 atcgacgcgc aggccgtcga taccgaagcg ctcgatccag tagagcgcgt tgccgaccag
     2161 atagttgcaa acctcgcgcc gcccgaagtt gtagatcagc gtgccccagt ccggatgaaa
     2221 gccctcgcgc ggatcggcgt gctcgaacag cggcgtgccg tcgaagcggc ctagcccatg
     2281 ctcgtcgctc gggaaatgcg ccggcaccca gtcggcaatg accttcagac cggccttgtg
     2341 gcaggagtcg acgaaagtcc taaaaccgct cgcggagccg tgtcgcgcgg tcggcgcata
     2401 caggccgagc ggctggtaac cccacgaccc gtcgaaagga tgctcggaga ccggcagcaa
     2461 ctcgatatga gtgaagccca gctcgaccac gtagggaatc agcgaagcgg ccaacgattg
     2521 ccaatcgaga aagctgccgt cggccttgcg tcgccacgag gcgaggtgca cttcgtagat
     2581 cgacaccggc gcgttcagcc gttcgccggc cggaatgcgt tctggcgcca ccgccggtgg
     2641 caggtcggca acgaccgagg cggtcgacgg ccgcagctcg gcggcgatac cgtagggatc
     2701 agccttgagc ggcagcaccg tgccatcggc gccgcgaatc tcgaacttgt aacgggcgcc
     2761 ggccgacacg ccgggcagga agatttccca gacgccacat tcgcggcgca gccgcatcgg
     2821 atggacgatg ccgttccagt ggttgaagtc gccgacgacg ctgacccgct gcgccgacgg
     2881 cgcccagacg gcaaaggccg tgccgccgac accatcgatc acgcaacgat gtgcgccaag
     2941 ccgctcgaag ggtcgccggt gcgagccttc ggcgagcagc cagacgtcca tttcgccaag
     3001 caccggcgga acacggtagg gatcatcgat ctcgaccgga ccctcgcccc agtcgacccg
     3061 caggcgatag tcgaaacaac gctttcgccg cgtcagcgcg ccttcgaaga acccggcagg
     3121 gtgcgtctgc ccgaggctgc cgaccagctt gccgcttgcg gcatcgacga tagcgacctc
     3181 gcgcgccatc ggcagtaggc accgaaccca cggatggcca tcatcgccgg cgtgcatgcc
     3241 gagcgcggca aagggatcgc cgcacgcacc gccggcgagc gcctcgacct cgctgcgggc
     3301 aagcgtcgtc atcagcgcgg ccggccgggc gcgatgtgcc aggtatcgac ggcgtactcg
     3361 cggatcgtcc ggtcggccga gaacggcccc atgccggcga tgttgagaat cgaggtgcgc
     3421 gcccaggcgg ccggctcgag atagagcgca tcgacgcgag cctgggtcga aatgtagtcg
     3481 gcgtagtcgg cgagcagcag gtaatggtca ccgtagcggg ccagcgcatt aaagatgtcg
     3541 tgatagcgcc ccggctcacc cggcgaaaaa aacccggtat cgatctggtc gagaacggtg
     3601 cgcagcgcgt cgttgtgtcg gtagatctcg tagggatcgt agccgttggc acgaatttcc
     3661 atcacctcgg cggcggactt gccgaagatg aagatgttgt ccgcgccgac ctggtcgcgg
     3721 atttcgatgt tggcgccgtc ttcggtgccg atggtcagcg cgccgttgag ggccagcttc
     3781 atgttgccgg tgcccgaagc ttcggtgccg gccgtcgaaa tctgctccga caggtcagcc
     3841 gccggcatga tgatctcggc gagcgagacg ccgtaattgg gcaggaaaac caccttcagg
     3901 cggtcgcgcg tgcgcggatc cttgttgacc acgctggcga catcgtggat cagccggatg
     3961 atcaacttgg ccatgtgata ggcgctcgct gccttgccgg cgaagatcac cgtgcgcggc
     4021 gcgaactcgg cgtccggatt ggccagcatc gcgttgtagc gcgtgatgac gtgcagcacg
     4081 ttgagcagct gccgcttgta ctcgtggatg cgcttgacct gcacgtcgaa gaggctcgcc
     4141 ggatcgacgt ggatgccctg cgtagtagcg atgtattcgg ccagccgcgc cttgttggcg
     4201 agcttggcgg cgacgcattc ggcctggaag tcggcgtcgc cggcaaaggt gcgcagcgac
     4261 gagaggcgat cgagatcgat acgccagccc ggatcgagcc tagcatcgat cgccgccgac
     4321 aggtcgcggt tggactgcgc cagccagcgc cgcggggtca cgccgttggt cttgttatgg
     4381 aacttggctg gatcaagctt cgcgaagtcg gcaaagatcg tctgcaccat caattccgag
     4441 tgcagcttcg agaccccgtt cacgcgatgg ctgccaacga tgcacaggtg cgccatgcgt
     4501 acgcgcttgt cgtgttcgtg tccgttgccc tcgtcgatca gcgaaacgcg gcgaatcaag
     4561 tcggaatcac cgggaaaacg ccgcgcgact tcgtcgagga attcctggtt gatgcggtag
     4621 atgatccgca tgtgccgtgg cagcacgtgc tggatcagcg tcagcggcca ggtctcgagc
     4681 gcctcgggca tcagcgtgtg gttggtgtag gaaaagatcc gccggcaact cgtccaggca
     4741 tctgcccacg ccaccgcgtg atcgtcgacg aggatgcgca tcagctcggc aacgccgatt
     4801 gctgggtgcg tgtcgttgag gtgaatcgcc accttatcgg ccagattgtc cagcgtgccg
     4861 tgctcgccca gatggcgggc gacgatgtcc tgcaccgaag cggcgacgaa aaaatactcc
     4921 tggcgcaaac gcaactcgcg tcctgccggc gtgctgtcgt tgggatagag cacccaggag
     4981 atgttctcga agtggttctt gaactcggca gcgcgcgcgt aatcgccctt gttgaaggcg
     5041 ccgagatcga tgtgcgtcgg cgccgctgcc ttccataggc gcaaggaact gacgcgggag
     5101 gtcgagtgac cggggatcac gtagtcgaag gctttcgcct cgacggcgtc ggccgcgtgc
     5161 cactcggccc agtcgccgtg atgatcgacg gtgccgccga agcggatcgt gtagtgcacc
     5221 tcgggtcgcg gaaactccca cggcgtaccg ttctccagcc aggagtcggg atgctcgacc
     5281 tgacagccgt tgtgcaccga ctgcgcgaac atgccgtact cgtagcgcat gccataaccc
     5341 caggagggca agccgagggt cgccatcgaa tcgaggaaac aggcggcaag ccgcccgagg
     5401 ccgccattgc cgagtgccgc gtcgggctcg gtggcaagca ccgcatcgat gtcgctgccg
     5461 cttccggcaa gtgccgctgc cgccgggtcg cgcaggttaa gcgcgtcgag cgcattgttc
     5521 agcgcgcgcc cgacgaggaa ttccatcgac agataataga tgcgccgcgc cttgaccgca
     5581 ctgtcctcgg actgcgtcgc cacccagcgt gcagccagct cctcgcgggc catgtaggcc
     5641 atcgcccgct gcgtctcgtc gctacccgcc gtcagtggcg cactggcgat
//
LOCUS       DATDAA010000245         3099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_472626,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000245 DATDAA010000000
VERSION     DATDAA010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472626"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /gene="dnaJ"
                     /locus_tag="VIS73_07780"
     CDS             complement(<1..986)
                     /gene="dnaJ"
                     /locus_tag="VIS73_07780"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY5763092.1"
                     /translation="MSKRDYYEVLGASKNASEADLKKAYRRAAQKYHPDRNPDDKVSE
                     EKFKECKEAWEVLGDAQKRAAYDQFGHAGVNPSMGGGGFGGAGAAGFGDIFGDVFGDI
                     FGGGRGGGGQRVYRGSDLRYNLELSLEDAVAGTEVKIRVPTLVECKTCSGSGARKGSE
                     PTACTTCGGHGQVRMQQGFFSVQQTCPHCQGSGSIISDPCPDCRGKGRVQEHKTLSVK
                     VPSGVDNGDRIRLAGEGEAGESGGPPGDLYVQIRVKEHPIFQREESNLYCEVPISFTT
                     AALGGELEVPTLNGRVNLKIPAETQSGKMFRMRGKGVKPVRGGPVGDLICRV"
     gene            complement(1102..3024)
                     /gene="dnaK"
                     /locus_tag="VIS73_07785"
     CDS             complement(1102..3024)
                     /gene="dnaK"
                     /locus_tag="VIS73_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY5763093.1"
                     /translation="MGKMIGIDLGTTNSCVAVMEGGEVRVIENSEGDRTTPSIVGFTS
                     EDEVLVGQAAKRQAVTNPTNTLYAVKRLIGRRFEEDVVQRDIGLVPYKIVKADNGDAW
                     VEAQGKKMAPPEVSARVLQKMKSTAEDYLGEAVTEAVVTVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAYGMDKKRGDSKIVVYDLGGGTFDVSIIEIADVDGEHQFEV
                     LSTNGDTFLGGEDFDKRLIDYLADAFQKEQGMDLRGDPLAMQRLKEGAEKAKIELSSS
                     QHTDVNLPYITADASGPKHLTMKLTRAKLESLVEDLIDRTIAPCKMALKDAGLSTSEI
                     DDIIMVGGQTRMPKVQEAVKDFFGKEPRRDVNPDEAVAVGAAIQGGVLGGDVKDVLLL
                     DVTPLSLGIETLGGVMTKLIEKNTTIPTKANQVFSTADDNQSAVTVHVLQGERDVATA
                     NKSLGRFDLSDIPPAPRGVPQIEVTFDIDANGILHVSAKDKATGKEQSIQIKASSGLS
                     DDEIDQMVKDAEAHAEEDRKFHELIDTRNQADGLIHATNKSLKDLGDKVSEDEKKDIE
                     AAIEELQAAMKGDDKAAIEAKTQALGEKSGKLAERVYKEQGAQEGAATAEAEAGAADD
                     DVVDAEFEEVKDADNK"
BASE COUNT          512 a         1053 c          856 g          678 t
ORIGIN      
        1 ctgacacgac agatcaggtc gccgaccggc ccgccgcgta ccggcttgac gcccttgccg
       61 cgcatgcgaa acatttttcc gctttgcgtt tcggccggaa ttttcaggtt gacgcgaccg
      121 ttcagtgtcg gcacttccag ctcgccaccg agcgcggccg tggtaaagct gatcggtact
      181 tcgcagtaga gattgctctc ttcccgctgg aatataggat gttccttgac gcggatctgc
      241 acatacagat caccgggcgg cccgccgctc tcgccggcct cgccctcgcc cgccaggcgg
      301 atacggtcgc cgttatcgac acccgacggc accttgaccg acagggtctt gtgttcctgc
      361 acccggccct tgccacgaca atccggacag ggatcggaaa tgatactgcc gctaccctgg
      421 cagtgcggac aggtctgctg cacggaaaag aacccctgct gcatgcgcac ctggccatga
      481 ccgccacagg tcgtacaggc ggttggttca ctgcccttgc gcgcaccact gcccgagcag
      541 gtcttgcact cgaccagggt cggcacacga atcttcacct cggtgccggc aacggcatct
      601 tccagcgaca attccaggtt atagcgcagg tcggaaccac gatagacacg ttgccccccg
      661 ccaccgcgac caccaccgaa gatatcgccg aagacatcac cgaaaatatc cccgaagccg
      721 gcggcacccg caccaccgaa accgccgcca cccatggacg gattgacacc ggcatggccg
      781 aactggtcat aggcggcgcg tttttgtgca tcgcccagga cctcccaggc ctccttgcac
      841 tccttgaatt tttcctcgga taccttgtcg tccgggttgc ggtccgggtg gtatttctgc
      901 gcagcacggc gataggcctt tttcagatcg gcctcgctgg catttttgga tgcgcccagc
      961 acctcgtaat aatcacgctt tgacatatgc tttatccact acgccttcgt actgtcccag
     1021 tatcaccccg ttaacggcaa aaatccgggg caggcaatgc cggccccgga tactcagtta
     1081 ctacctgtcc cggacttgcc gctacttgtt gtcagcatcc ttcacttctt cgaactcggc
     1141 atcgaccaca tcatcgtccg ccgcgcccgc ttccgcttca gccgttgcag cgccttcctg
     1201 tgcgccctgt tccttgtaaa cacgttcggc cagcttgccg gacttctcgc ccagcgcctg
     1261 cgtctttgcc tcgatggccg ccttgtcgtc gcccttcatg gccgcctgca gttcctcgat
     1321 ggcggcctcg atgtccttct tctcgtcttc cgagaccttg tcaccgagat ccttcagcga
     1381 cttgttggtc gcgtggatca ggccgtccgc ctggttgcgc gtatcgatca gttcgtggaa
     1441 cttgcggtct tcctcggcat gcgcctcggc gtccttcacc atctggtcga tctcgtcatc
     1501 cgacaggcca ctggaggcct tgatctggat cgactgttcc ttgccggtgg ccttgtcctt
     1561 tgctgacacg tgcaggatgc cgttggcgtc gatgtcgaac gtcacctcga tctgcggcac
     1621 gccgcgcggg gccggcggga tatcactgag gtcgaaacgg cccagcgact tgttggccgt
     1681 ggccacgtca cgctcgccct gcagcacatg cacggtcaca gccgactggt tgtcatcggc
     1741 cgtggagaac acctggttcg ccttggtcgg gatggtcgta ttcttctcga tcagcttggt
     1801 cattacgccg cccagcgtct cgatacccag cgacagcggg gtcacatcga gcagcagcac
     1861 gtccttgaca tcgccgccca gcacaccgcc ctggatggca gcgccgacgg cgacggcctc
     1921 gtccgggtta acatcgcggc gcggctcctt gccgaagaaa tccttcacgg cctcctgcac
     1981 cttcggcata cgcgtctggc caccgaccat gatgatatcg tcgatttcgc tggtggacag
     2041 gccggcatcc ttcaatgcca ttttgcaggg tgcaatagta cggtcgatca ggtcttcaac
     2101 cagagactcg agcttggcac gcgtcagctt catggtcagg tgcttcggac cgctggcatc
     2161 ggccgtgatg tacggcaggt tcacatcggt atgctgactg gaagacagtt cgatcttggc
     2221 cttctcagca ccctccttga ggcgctgcat ggcgagcgga tcaccgcgca ggtccatgcc
     2281 ctgctctttc tggaacgcat ctgcgaggta gtcgatcagg cgcttgtcga agtcctcacc
     2341 accgaggaaa gtgtcgccgt tggtcgacag cacttcgaac tggtgttcac cgtcgacatc
     2401 cgcgatttcg ataatcgaca cgtcgaaggt gccgccaccg agatcgtaca cgacgatctt
     2461 ggaatcgccg cgtttcttgt ccatgccgta ggcaagggcc gcagccgtcg gttcgttgat
     2521 aatgcgcttg acgtcgagac ccgcgatacg gccggcatcc ttggtcgcct ggcgctgcga
     2581 gtcattgaag taggccggca ccgtaacaac ggcctcggtc actgcctcgc cgaggtaatc
     2641 ttccgccgtg cttttcattt tctgcagcac gcgggcggac acctcgggcg gcgccatctt
     2701 cttgccctgc gcctcgaccc aggcatcgcc gttgtcggcc ttgacgatct tgtagggcac
     2761 caggccgatg tcgcgctgca cgacatcttc ctcgaagcgg cggccgatca ggcgcttcac
     2821 cgcatacagt gtatttgtcg gattggtgac ggcctggcgc ttggcggcct ggccgaccag
     2881 cacctcgtct tcactggtga agccgacaat cgacggcgtg gtgcgatcgc cctcgctgtt
     2941 ctcgatcacc cgaacctcgc caccttccat caccgccacg caggagttgg tcgtgcccag
     3001 gtcgattccg atcatctttc ccacgtcgaa cctcgctccc ttcgtgtgcc ggacgggcta
     3061 gtcgtccccg tcctcgccac cgccctcgtg cccgtcctc
//
LOCUS       DATDAA010000246         2683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_17835,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000246 DATDAA010000000
VERSION     DATDAA010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17835"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VIS73_07790"
     CDS             <1..280
                     /locus_tag="VIS73_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763094.1"
                     /translation="PDTTDVGYLFAEARQRLADKQVLLGCARPPGLHKRVTDAYAIMA
                     GLDGIAFPADGAVSVAHRIGRPFEQAHSCCSIKLGSDFKQRLARSCAA"
     gene            299..1552
                     /locus_tag="VIS73_07795"
     CDS             299..1552
                     /locus_tag="VIS73_07795"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5763095.1"
                     /translation="MGCIENRSTPNEAAGETDFNSFSKMAPRSPQRTPVDRKNGRHVK
                     ANDIPPQGVYLPNNNYMAPHMKSPEYVQLSTAAAITLGIMGGRMYGCECTRCLNLLLT
                     YPEGCRANCAYCGLARHREADRDYADRNFIRVDWPAVPMAEIVDIVSRDPDSSPFHRM
                     CISMITHPRSEDDTFTVLKQWTDRIDPDAIPVSILSNPTTMTRADVQKTRDLGADIFT
                     VALDAATPEVFDRTRGKGVQSPHKWSKYWEILEDARDIFGPEKFGAHIIVGMGETEYE
                     VLELVQRLVDMGGHSHMFCFFPEQGSLMDHLPATPRDQWRRVQLARYLIDYRGVRVEQ
                     MSFDETGRVRRYGIPEAELDEVISEGVAFRTSGCPGKIRDDISACDRPYGDSPPSDIA
                     SYPFQPGKKDLRRIRRQLDIPVRIE"
     gene            1563..>2683
                     /locus_tag="VIS73_07800"
     CDS             1563..>2683
                     /locus_tag="VIS73_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HEY5763096.1"
                     /translation="MPGEFRIIDTGVREGRANIAFDAALIEERQAGRVPDTIRFLSFP
                     PTALIGRHQDLSRELDLDYCRANGVGTVRRITGGGAIYLDEGQLGWELVFHRASLGIA
                     ALPDLARAICEAAASGLSRLGVDARFRPRNDIEVDGRKISGTGGFFDGDVLIYQGTVL
                     VDLDPAKMVGALRVPQSKLAKRNLDSAAQRGVTLRELLGDRLPELETIRGALVAGFEE
                     GLGITTAPGDITDAEEALARAFFDEEIGTDEFVAEIDNPAAEARTLGGSHTGPGGTVD
                     AFVRLEGPTSEILQRVLITGDFFVTPPRTVFDLEARLQGTRIDEIEAAIDDFFGRTEI
                     DTLSVTAEDFKAAITGAVAKREVEHACYRCLEVFAMADRG"
BASE COUNT          492 a          891 c          873 g          427 t
ORIGIN      
        1 tccggatacg accgacgtcg gctacctgtt cgccgaagcc aggcagcggc tcgcggacaa
       61 acaggtactg ctcggctgcg cacggccgcc gggcctgcac aaacgcgtta ccgacgccta
      121 cgccatcatg gcgggccttg acggcattgc gttcccggcc gacggcgcgg tttcggtcgc
      181 gcacaggatc gggcggccgt tcgaacaggc gcactcctgc tgctccataa aactgggcag
      241 tgatttcaag caaaggctcg cgcgatcctg cgcggcgtaa tctgcaggta ccgattccat
      301 gggttgcatc gaaaacaggt ccacgccgaa cgaagcagcg ggagagacgg acttcaattc
      361 cttctcgaaa atggcgccgc gttcgccgca gcggaccccc gtcgaccgta agaacgggcg
      421 ccacgtcaag gccaacgaca ttccgccgca gggcgtctat ctgccgaata acaactacat
      481 ggccccgcac atgaagtcgc ccgagtacgt gcagctgtcg accgccgcgg ccattacgct
      541 cggcatcatg ggcggccgca tgtatggctg cgaatgcacc cgctgtctca acctgctgct
      601 gacctacccg gaaggctgcc gcgccaactg cgcctactgc ggtctcgcgc gtcatcgcga
      661 ggcggaccgg gattacgcgg accggaattt catccgggtc gactggccgg ccgtgccgat
      721 ggcggagatc gtcgatatcg tgtcgcggga tcctgattcc agcccctttc atcgcatgtg
      781 catttcgatg atcacgcacc cgcgctcgga ggacgatacg ttcaccgtcc tgaagcagtg
      841 gaccgaccgc atcgatccgg acgcgatccc cgtatccatc ctctccaacc ccacgacgat
      901 gacgcgcgcc gacgtgcaga agacgcgcga tctcggcgcc gacatattca ccgtcgcgct
      961 ggacgcggcg acgcccgaag tgttcgaccg cacccggggc aaaggcgtgc agtcgccgca
     1021 caagtggagc aagtactggg aaattctcga ggacgccagg gacatattcg ggccggaaaa
     1081 attcggcgca cacatcatcg tcggcatggg cgagaccgag tacgaggtgc tcgagctcgt
     1141 ccagcgcctc gtcgacatgg gcggccacag tcacatgttc tgcttcttcc cggagcaggg
     1201 ctcgctgatg gatcacttgc ccgctacgcc gcgcgaccag tggcggcgcg tgcagctggc
     1261 gagatacctc atcgattatc gcggtgtgcg cgtggagcag atgagcttcg acgaaacggg
     1321 ccgggtgcgg cgctacggca ttccggaggc ggaactcgac gaggtcatca gcgagggtgt
     1381 cgccttccgg acttcggggt gccccggcaa gattcgtgac gatatctccg cgtgcgacag
     1441 gccatacgga gactcgccgc cgagcgacat cgcgagctac ccgttccagc ccgggaagaa
     1501 ggatcttcgc aggattcgcc ggcagctcga catcccggtg cggatcgaat agcggttcgc
     1561 gaatgcccgg cgaattcagg atcatcgaca ccggcgtgcg cgaagggcgc gccaatatcg
     1621 ctttcgacgc cgccctgatc gaagagcggc aggccggcag ggtcccggac acgatccgct
     1681 tccttagctt tccgccgacc gcgctgatcg gccggcacca ggacctgagc cgcgaactcg
     1741 acctcgacta ttgccgggcc aatggcgtcg gcacggtccg ccggataacc ggcggcggcg
     1801 ccatctacct ggacgagggc cagctcggct gggaactcgt ctttcatcgc gcatcgctcg
     1861 gcatcgcggc attgcccgat ctcgcccggg ccatctgcga ggcggccgcg tccggcctgt
     1921 cgaggctcgg cgtcgacgcc cgcttccggc cgaggaacga catcgaggtc gacggccgca
     1981 agatcagcgg cacgggcggt tttttcgacg gcgacgtgct gatctaccag ggcaccgtgc
     2041 tcgtggacct cgaccctgcg aagatggtcg gcgcgctgcg cgtgccgcaa agcaagctcg
     2101 ccaagcgcaa tctcgactcc gccgcccagc gcggggtgac gctgagggag ctgctgggcg
     2161 accggctgcc ggaactcgaa acgattcgcg gcgcgctggt tgccggcttc gaggagggcc
     2221 tcggcatcac gactgccccg ggagacatca ctgacgccga ggaggcgctg gcgcgagcgt
     2281 ttttcgacga ggaaatcggc accgacgagt tcgtcgccga gatcgacaac ccggccgccg
     2341 aggcacgaac gctcggcggc agccacaccg gccccggggg aaccgtcgac gccttcgtca
     2401 ggctggaggg gccgaccagc gagatcctgc agcgggtgct cataaccggc gacttcttcg
     2461 tcacgccgcc ccgaaccgtg ttcgatctcg aggcgcggct gcaggggacg cgcatcgacg
     2521 agatcgaggc cgcgatcgac gacttcttcg ggcggaccga aatcgacacg ttgagcgtca
     2581 cggccgaaga cttcaaggca gcgataacag gcgccgtcgc gaaacgggag gtcgaacatg
     2641 cctgttatcg ctgccttgaa gtcttcgcca tggcggaccg cgg
//
LOCUS       DATDAA010000247         7180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_262687,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000247 DATDAA010000000
VERSION     DATDAA010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7180
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262687"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VIS73_07805"
     CDS             complement(<1..650)
                     /locus_tag="VIS73_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763097.1"
                     /translation="MRKNRLIAAARIAALGAAGAAAVLTTASASAQGYASLAEGRAVF
                     DQVGCNACHSYVSRRTAPSIRDLMRDFSASPTAIADATRGSRAHRDDAQFPRVSDADL
                     RSISEWLAGISWPADTPVASSAPSGNFLADKAPGDGLTSFEMAGCNECHGFSARAKAP
                     SIRNMKQEFATEPLAVAQATRGNNAHRDDNRIRDLADIHLRNIAEYISDTPWPESLL"
     gene            complement(647..1534)
                     /locus_tag="VIS73_07810"
     CDS             complement(647..1534)
                     /locus_tag="VIS73_07810"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEY5763098.1"
                     /translation="MKLRLKFNLMLIAVFGLALTAAGFLSFRQLSENAEHEVLSNAGV
                     LMESLLSARTWAVVEVGPNLREIEEGEDYLMMGVPPYASKKVMAGIQKRYPDYKYREL
                     ALNPLNLKDHYPEDWERQIINLFRIDQNLKEQWGYRYTPEGKFLYLARPIQIDRAGCL
                     GCHSTPEKSPPEIVAMYGKDSGYGWQRDEIVGMQMVMVPTSLPEANAQRMFVTFMTTI
                     GSVFFILFLLINWMLETLVVRPINRMAESADEISKGNLKVAEFNESGKDEVSHLAASF
                     NRMKRSLEKAMALFYSKSE"
     gene            complement(1833..2189)
                     /locus_tag="VIS73_07815"
     CDS             complement(1833..2189)
                     /locus_tag="VIS73_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5763099.1"
                     /translation="MSDPTAAPEPIPEVLARLLKLVGGPRDNALLHHAIGSEWMKAGR
                     PREAAAALHTALARDPNFSAAWKLLGKALLEDGDNDAACAAWQQGIVVAEARGDVQAA
                     KEMQVFLRRVGRATGA"
     gene            2238..2618
                     /locus_tag="VIS73_07820"
     CDS             2238..2618
                     /locus_tag="VIS73_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY5763100.1"
                     /translation="MARSIVSTPDAPAAIGPYSQAVRVGDTVYLSGQIGLDPKTMEMV
                     EGIDAQIDRVFANLQAVATAAGGSFSDIVKLNVFLTDLGHFAKVNEKMSRHFVEPYPA
                     RAAIGVAALPRGALVEMDAVLVLG"
     gene            2658..4718
                     /gene="recG"
                     /locus_tag="VIS73_07825"
     CDS             2658..4718
                     /gene="recG"
                     /locus_tag="VIS73_07825"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY5763101.1"
                     /translation="MPPRARKAANAKPAGIADKFARLGLRRPIDFVLHLPHRYEDETE
                     LLPLDGLPAFGLVQTEGAVRDVAVQFRPRRQLVVHLADDSGSNLTLRFLNFYGSQSKQ
                     FETARESGSLVRVFGEVRGGFFGAEMVHPRYRVVAPGTPLPSVLTPVYPVTAGVSQAA
                     IRKRIAAALGSEGLDETLPPDACAELPPLRAAVGRLHAPPADAAITALNAGDDPAWAR
                     VRFDELVAQQLSLRRAYRARRARGAFPLQATGRLTDRLLAALPFPLTAAQRRVWAEVA
                     ADLAQEYPMQRLLQGDVGSGKTLVAALAALHAIEAGTQAAFMAPTEILAEQHALKLES
                     WLAPLGLTVVHLSGAMRSATKRAKQAEIAAGADLVIGTHALIQDSVDFSRLGLAIVDE
                     QHRFGVAQRLALRKKGERDGVLPHQLMMSATPIPRTLAMSYFADLDVSVIDEMPPGRT
                     PVRTRLVADARRDEVIERVRAACSGGRQAYWVCPLVEESDKVELQAALDTHARLVEEL
                     PELSIGLVHGRMKGDEKAAAMAAFSQGTTQLLVATTVIEVGVDVPNASLMVIEHAERF
                     GLSQLHQLRGRVGRGAVESTCVLLFANPLSENARERLKVIFEHQDGFEIARRDLALRG
                     PGEFIGTRQSGVPMLRYADLERDAHWLDRARELAEQMLADDPEAASRHLDRWFGGREE
                     LLKA"
     gene            4867..6174
                     /locus_tag="VIS73_07830"
     CDS             4867..6174
                     /locus_tag="VIS73_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5763102.1"
                     /translation="MSSTLHLLKVRRFAPLFVTQFFGAFNDNVFKNALVVLLTFKAAN
                     WSDLAPAVLANLAAGLFILPFFLFSASAGQWADKTDKARMARLTKLLEVLIVLVAGLG
                     FWLQNLPLLFIALFLLGAQSALFGPVKYAVLPQHLRADELLGGNALIEAATFVSILLG
                     TILGGVLAALDQGTLWITATSLLVAVVGLVASLAIPPAPPPAPELVINRNPLAETWRT
                     VAALPAQAAVFTAVLGISWFWLYGALLLAQFPAFGRYVLGGDEGVVTLMLAVFTVGIG
                     VGSMACAQLSRGRLSLRWVGPGALGITVATLDLAWAAAHFPLGETLRPVVAWLGSGAS
                     WRILADLLVIGSAGGLFIVPLYTQLQLRAEEKSRARAIAANNIVNALFMVVGALVAGA
                     LLGGGLSIPALFAGAALLNLAALLLLYRRDPAFVAAWRNARDR"
     gene            6223..7122
                     /gene="ubiA"
                     /locus_tag="VIS73_07835"
     CDS             6223..7122
                     /gene="ubiA"
                     /locus_tag="VIS73_07835"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEY5763103.1"
                     /translation="MTDLASPPSRRARCFARIHDYARLMRLDKPIGILLLLWPTLWGV
                     WLAADGRPAWTIVLIFVSGTVLMRSAGCVINDYADRDFDPEVARTKQRPLAARRVSVR
                     EALTLFVVLVLLAFLVMLPILTWPLLAMAIAALFLAASYPFTKRFFAVPQAYLGVAFG
                     FGIPMAWLAQTGAVAPAAWMLLVANIFWAIAYDTEYAMVDRADDLRIGIRSSAITFGR
                     FDVAAVAACYALALALLVVVGQMLASGWPYFLALLLAGVIAVTHVRRIAAREPGECFR
                     VFLDNNRIGAIVFAGIVIDSLLS"
BASE COUNT         1070 a         2325 c         2391 g         1394 t
ORIGIN      
        1 agcagggatt ccggccaggg cgtgtcggaa atgtactcgg cgatgttgcg caggtggatg
       61 tcggcgaggt cgcgaatccg gttgtcgtcg cggtgagcat tgttgccgcg cgtcgcctgc
      121 gcgacggcga gcggttcggt cgcgaattcc tgcttcatgt tgcggatcga cggcgccttg
      181 gcgcgcgccg aaaaaccgtg gcactcgttg cagccagcca tctcgaagct cgtcaggccg
      241 tcgccgggtg ccttgtcggc gaggaaattg ccgctcggcg cactactggc gaccggggtg
      301 tccgcgggcc agctgatgcc ggccagccat tcggagatgc tgcgcaggtc ggcgtcgctg
      361 acccggggaa actgtgcatc gtcgcggtgt gcccgactgc cgcgcgtcgc gtcggcaatc
      421 gcggttgggc ttgccgagaa gtcgcgcatc aggtcgcgta ttgacggcgc ggttcggcgc
      481 gacacatagg agtggcatgc gttgcagcca acctggtcga agaccgcgcg gccttcggcg
      541 aggcttgcat aaccctgcgc cgatgcagat gccgtcgtta acactgccgc agctcccgcg
      601 gctcccagcg cggcgatacg tgccgccgcg atcagtcgat tttttctcat tcggatttgc
      661 tgtagaacag ggccatggcc ttttcgaggc tgcgtttcat ccggttgaag gaagcggcga
      721 ggtgagagac ttcgtccttg ccgctttcgt tgaactcggc aaccttcagg ttgcccttgc
      781 tgatctcgtc tgcagattcg gccatacggt tgatcgggcg aacgaccaga gtctccagca
      841 tccagttgat gagcagaaag aggatgaaga acaccgagcc gattgtcgtc atgaaggtca
      901 cgaacatacg ttgggcgttg gcctcgggca gcgaggtcgg caccatgacc atctgcatgc
      961 cgacgatttc atccctctgc cagccgtaac ccgagtcctt tccgtacatt gcgacgattt
     1021 ccggcggtga cttttcgggg gtcgagtggc agccgagaca gccggcgcga tcaatctgaa
     1081 ttggccgggc gaggtagagg aacttgcctt ccggggtata gcggtagccc cactgttcct
     1141 tgaggttctg gtcgatgcgg aagaggttga tgatctgccg ctcccaatcc tcaggatagt
     1201 gatccttgag gttcaacggg ttcaatgcga gttcgcggta cttgtagtcg ggatagcgct
     1261 tctggatgcc ggccatcacc ttcttgctgg cgtagggggg gacgcccatc atcaggtagt
     1321 cctcgccctc ttcgatctcg cgcaggttgg ggcctacttc gacgactgcc caggtccgcg
     1381 ccgagagcag cgactccatc agcacgcccg cgttggagag gacctcgtgc tcggcgtttt
     1441 ccgacagctg ccgaaacgag aggaatccgg cagcggtcag cgccagtccg aaaacggcga
     1501 tgagcatcag gttgaactta aggcgcagct tcatggcatt ggtcccgcta gagataacgg
     1561 cgggcgtaac gataacatga ggcgatacgc ggggtacgga caattatttc cgcatttcga
     1621 ccgattggtg cggctttcgg acgggtcggc cgacgccgtc gcggcacgca tttcaccgtc
     1681 gcacgacaag gtggctcgca tcggccgccg cgcagaatgt gctcacgcga taccgaaacg
     1741 attcggcagg ccgcatcggc cgcgtatccg gctgacggtg cacagggtta tccccagatt
     1801 ctgtggataa ccgccatcgg tgattgcggt tatcaggcgc cggtggcgcg accaacgcgg
     1861 cgcaagaaga cctgcatctc cttcgcggcc tgcacgtcgc cgcgtgcctc ggcgacaacg
     1921 atgccttgct gccaggcggc acacgcggcg tcgttatcgc cgtcttcgag cagggccttg
     1981 cccagcagtt tccacgcggc cgaaaaattc gggtcgcggg cgagtgcggt gtgtagcgct
     2041 gccgccgctt cgcgcggccg cccggccttc atccactcgc tgcctatcgc atggtgaagc
     2101 agggcgttgt cgcgcggccc gccgaccagc ttgagcaggc gcgcgaggac ttcgggaatc
     2161 ggctcgggtg cggcggtggg gtcggacata gcggctacaa tcgtggcaga cataaccatt
     2221 cttgtgaagg agctctcatg gcgcgcagca tcgtttcgac ccccgacgct ccggcggcaa
     2281 tcggccccta ttcgcaggcc gttcgcgtcg gcgacacggt ctacctctcg gggcagatcg
     2341 gtctcgatcc gaagaccatg gaaatggtcg agggcatcga tgcgcagatc gatcgcgtct
     2401 tcgccaacct gcaggcggtg gctaccgcag ctggcggctc cttttccgac atcgtgaaac
     2461 tcaacgtctt cctcaccgac ctcggccact ttgccaaggt caacgagaag atgtcgcgtc
     2521 acttcgtcga gccctatccg gcgcgtgcgg cgatcggcgt cgcggcgctt ccgcgcggcg
     2581 cgctggtgga gatggatgcg gtactggtgc ttggctgagg cgcaagcccg tcgccgccgc
     2641 gcgccgcggg tcggctgatg ccgccgcggg cgcgcaaggc cgccaacgcc aagccggccg
     2701 gcatagccga caagttcgcc cgcctcggtc tgcggcggcc gatcgatttc gtccttcatc
     2761 tgccgcatcg ttacgaagac gaaaccgagc tgctgccgct agacggtctg ccggccttcg
     2821 gcttggtgca gaccgaagga gcggtgcgtg atgtcgccgt acagttccgt ccacgccggc
     2881 aactggtcgt gcatctggcc gacgattccg gttcgaatct gacgctgcgc tttctcaact
     2941 tctacggcag ccagagcaag cagttcgaga cggcgcgcga gagcgggtcg ctggtccgcg
     3001 ttttcggcga agtgcgaggc ggtttcttcg gcgccgagat ggtccatccg cgctatcgcg
     3061 tcgtggcgcc gggaacgccc ttgccatcgg tgttgacgcc ggtctatccg gtgactgccg
     3121 gtgtctcgca ggcggcgatc cgcaaacgca ttgccgccgc actgggcagc gaggggctcg
     3181 acgagacgct gccgcctgat gcctgcgccg aactgccacc gctgcgcgcc gcggtcggga
     3241 ggctgcatgc accgccggct gacgcggcga tcactgcgct caatgctggc gacgacccgg
     3301 cctgggcgcg cgtgcgcttc gacgaactgg ttgcccagca actgtcgctg cgtcgggcct
     3361 atcgcgcgcg gcgcgcgcgc ggcgccttcc cgttgcaggc gacggggcga ctcaccgatc
     3421 gcctgctggc ggcgctgccg tttccgctga ccgcagcgca gcgccgcgtc tgggccgagg
     3481 tcgccgccga tctggcgcag gaatatccga tgcagcgact gctgcagggc gatgtcggca
     3541 gcggcaagac cctggtcgcg gcgctggccg ctctgcacgc catcgaagcc ggaacgcagg
     3601 cggccttcat ggcgccgacc gagatcctcg ccgaacagca cgcgctgaag ctcgaatcct
     3661 ggctggcgcc gcttggcctg acggtcgtcc atctgtcggg tgcgatgcga agcgccacca
     3721 agcgggcgaa gcaggccgag atcgccgccg gtgccgacct cgttatcggc acccacgcgc
     3781 taatccagga ttcggtcgac ttctcgcgcc tcggtctggc aatcgtcgac gagcagcatc
     3841 gtttcggtgt cgcgcagcgg ctggcgctgc gcaagaaggg tgagcgcgat ggcgtactgc
     3901 cgcaccagct gatgatgtcg gcgacgccga ttccgcgcac gctggcgatg agttacttcg
     3961 ccgacctcga tgtctcggtg atcgacgaga tgccgcccgg gcgcacaccg gtacgcacac
     4021 ggttggtcgc cgacgcgcgc cgcgacgagg tgatcgaacg cgtgcgcgca gcctgctccg
     4081 gtggccggca ggcctactgg gtctgcccgc tggtcgagga gagcgacaag gtcgaactgc
     4141 aggccgcgct cgatacccat gcgcggttgg tcgaggaact gcccgagctg tccatcggtc
     4201 tcgtccatgg gcggatgaag ggcgacgaga aggcggcggc gatggcggct ttcagccagg
     4261 gcacgacgca actactggtg gcgacgactg tgatcgaggt cggtgtcgac gtgcccaatg
     4321 cgagcctgat ggtgatcgaa cacgccgagc gtttcgggct gtcgcagctg caccagctgc
     4381 gcggccgcgt cggccgtggt gccgtcgaga gcacttgcgt gctgctcttt gccaacccgc
     4441 tgtcggaaaa cgcaagggag cgattgaagg tgatcttcga gcatcaggac ggattcgaga
     4501 tcgcgcgccg cgaccttgcg ctgcgcggcc ccggcgagtt catcggcacg cgccagagcg
     4561 gcgtgccgat gttgcgctac gccgacctcg aacgcgacgc acactggctg gaccgtgcgc
     4621 gcgaacttgc cgagcagatg ctcgccgacg accccgaggc cgccagccga catctcgacc
     4681 gctggttcgg tggccgcgag gagttgttga aggcatgaac gtcgcgaatt tagaggcggt
     4741 acgcacggtg tatatcccgc gccatcgaca gacagtccgt aaagtggccc aacctgattc
     4801 gccctgcggt gaagcggcga gctaccttcg ctcattgccg tcgggcaggg gccgggatga
     4861 cggctcatga gctctacgct gcacctgctg aaagtccgcc gtttcgcgcc ccttttcgtg
     4921 acgcaattct tcggtgcctt taacgacaac gtgttcaaga acgcactggt tgtgctgctg
     4981 accttcaagg cggcgaactg gagcgacctg gcgccggcgg tactggccaa tctggcagcc
     5041 ggcctgttca tcctgccctt cttcctgttc tcggcgagcg ccgggcagtg ggccgacaag
     5101 accgacaagg cgcgcatggc gcggttgacc aagctgcttg aggtgctgat cgtgctcgtc
     5161 gccggactcg gtttctggct gcagaacctg ccgctgctgt tcattgccct gttcctgctg
     5221 ggagcgcagt cggcgctgtt cggccccgtc aagtacgctg tcctgccgca gcacctgcgc
     5281 gccgacgagc tgctcggcgg caatgccctg atagaagcgg cgaccttcgt ctccatcctg
     5341 ctcggcacca tcctcggcgg cgtcctcgcg gcgctcgacc agggaacgct gtggatcacc
     5401 gcgaccagcc tgctggtggc cgtcgtcgga cttgtcgcca gcctggcgat tccgccggcg
     5461 ccgccgccgg cgcccgaact ggtgatcaac cgcaatccgc tggccgagac ctggcgcacg
     5521 gtcgccgcat tgccggcgca ggcagcggtt ttcaccgccg tgctcggcat ctcctggttc
     5581 tggctctacg gcgcgctgct gctcgcgcag tttccggcct tcggtcgcta cgtgctcggc
     5641 ggtgacgaag gcgtggtgac gctgatgctc gcggtgttta cggtcggcat cggcgtgggc
     5701 tcgatggcct gtgcgcaatt gtcgcgcggc cggctttcct tgcgctgggt gggtccgggg
     5761 gcgctcggga tcaccgttgc gacgctcgat ctcgcctggg cggcggcgca ctttccgctc
     5821 ggcgaaacgc tgcggccagt cgtggcttgg ctcggctccg gcgcgagctg gcgcatcctc
     5881 gccgacctgc tggtgatcgg ttctgccggc ggcctcttca tcgtgccgct gtatacgcaa
     5941 ctgcaactgc gtgccgagga gaaaagccgg gcccgggcaa ttgccgccaa caacattgtc
     6001 aacgcgctgt tcatggtggt cggcgcgctc gtcgccggcg cgctgctcgg cggcgggctg
     6061 tcgattccgg ccttgttcgc cggcgcggcg ctgctcaatc tggctgcgct gctgttgctc
     6121 taccgtcgcg atccggcctt cgtcgccgct tggcgaaacg cgcgtgaccg ctagaatgcg
     6181 gccctcgatg tttcagcgtt tacctgttcc gtccatcccg caatgaccga tctggcgagc
     6241 ccacccagcc gacgcgcgcg ttgtttcgcc cgcatccacg actacgcgcg gctgatgcgg
     6301 ctcgacaaac cgatcggaat cctgctgctg ctctggccca cgctctgggg tgtctggctg
     6361 gcagccgacg gccggcctgc gtggacgatc gtgctgatct tcgtttccgg gaccgtgctg
     6421 atgcgctcgg ccggctgcgt catcaacgat tacgccgatc gtgattttga ccccgaggtg
     6481 gcgcgcacga agcaacgtcc gctggcggcg cggcgcgttt cggtgcgcga ggcgctgaca
     6541 ctgtttgtgg tgctcgtgct gcttgccttc ctcgtgatgt tgcccattct cacttggccg
     6601 ctgctggcga tggccatcgc cgcgctattt ctcgcagcga gctatccgtt caccaagcgc
     6661 ttcttcgcgg ttccgcaggc ctacctcggc gtcgccttcg gcttcggcat cccgatggcc
     6721 tggctggctc agaccggcgc ggtcgcgccg gccgcctgga tgctgctcgt cgccaatatc
     6781 ttctgggcga tcgcctacga caccgagtac gcgatggtcg atcgggccga cgatctgcgg
     6841 atcggcatcc gcagttcggc gatcaccttt ggccgctttg acgtcgctgc ggttgccgcc
     6901 tgctatgcat tggccctggc actgctggtg gtggtcgggc agatgctggc gagcggctgg
     6961 ccatatttcc tcgcgctgct gctggccggc gtgatcgcgg tcacgcatgt gcgccgcatc
     7021 gccgcacgcg aacccggcga atgcttccgt gtttttctcg acaacaaccg catcggtgcc
     7081 atcgtctttg ccggcatcgt catcgactcg ttgctcagct gatgcccaga attcttgttt
     7141 cgaccctgct cggccttctg ctcgctgcct gcacgcaacc
//
LOCUS       DATDAA010000248         2043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_87864,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000248 DATDAA010000000
VERSION     DATDAA010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87864"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VIS73_07840"
     CDS             <1..916
                     /locus_tag="VIS73_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763104.1"
                     /translation="GAFGARSRGALDMDHDTRQLRDRRGRELLVVRSNFRVLAMKYWL
                     SLSLFVACSLPVAAFAQGDENLAKKLANPVAALISMPIQANYDEDFGLDDEGAVLRIN
                     VQPVIPFSIGPDWNLISRTILPVLNFDDVPVPGTSEFGLGDTVQSLFFSPKAPTASGW
                     IWGAGPVLLIPTATDEVLGSEKWGIGPTAVVLRQAGPWTYGALANHIESFAGTSRRAD
                     VSATFIQPFLTYVTPTQTTFALNTESTYDWETNKWSVPINLTAAQMLRFGNQLVQIGG
                     GLRYWVNTPDTGPEDWGLRLQFTLLFPR"
     gene            1040..1699
                     /locus_tag="VIS73_07845"
     CDS             1040..1699
                     /locus_tag="VIS73_07845"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5763105.1"
                     /translation="MAEDLHLNPLTGHDEFDRLIAEFSELRDAEAMESARQGIWERFG
                     VEGAVFISDMASFSSTSRKIGVCHFLKLIHRARQIIAPLVDRYNGLLLKCDADNCYAF
                     FESTDDAIRASFDINAELFRHNDEFSLSEQIHLSIGIDYGQVLLIGDREFFGDPVNTA
                     SKLGEDLAVRSETLITRRALERATLMIPETAEHMVARISDIEIRYVRLPMTEIERDEL
                     G"
     gene            1696..>2043
                     /locus_tag="VIS73_07850"
     CDS             1696..>2043
                     /locus_tag="VIS73_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY5763106.1"
                     /translation="MSRRLSEAQALRSVLEVTRKLGAPFDLDTMLTEVVDAARRVLDA
                     DRGTVFLYDDNSNELVVRVGTKLGSIRIPADKGIVGESAMSRKLINVPDCYEDERFNR
                     AIDKETGYRSRCML"
BASE COUNT          437 a          605 c          609 g          392 t
ORIGIN      
        1 gggtgctttc ggcgcaagat cacgaggtgc actggacatg gatcacgaca cccgccagtt
       61 acgtgatcgg cggggtcgcg agttactggt tgttcgatcg aatttcaggg ttctggctat
      121 gaaatactgg ttgtctctat cgctttttgt tgcttgctct cttcccgttg cggccttcgc
      181 ccagggcgac gagaacctgg caaagaagct ggccaacccg gtcgcggcgc taatcagcat
      241 gccgatccag gcgaattatg acgaggactt cggcctcgac gacgagggcg cggtgctgcg
      301 aattaatgta caacccgtga tcccgttctc gatcggaccg gactggaacc tgatttcccg
      361 cactatcctg ccggtcctga acttcgacga tgtacccgta ccgggtacca gcgagttcgg
      421 gctgggcgac acggtgcaga gcctgttctt ctcaccaaaa gccccgaccg cgagcggctg
      481 gatctggggt gcgggcccgg ttctcctgat tcctaccgca acggacgaag ttctcgggag
      541 cgaaaagtgg ggtatcggcc cgaccgccgt cgtgctgcgg caggcaggcc cctggaccta
      601 cggtgcactg gccaatcaca tcgagtcgtt cgcgggcacg agccggcgcg cggacgtcag
      661 cgcgacattc atccagccct tcctgaccta cgtcaccccg acgcagacaa cgttcgcgct
      721 caataccgag tcgacctacg actgggagac caacaaatgg tcggtgccga tcaatctcac
      781 cgccgcacag atgctgcgct tcggcaacca gctcgtgcag atcggcggcg gcctgaggta
      841 ctgggtcaat acgcctgaca caggcccgga ggactggggt ttgaggttgc agttcacact
      901 gctcttcccg agatagatta aagagcacgt acgcggaaaa agcgggctgc cctgtagccg
      961 cgcggcaaaa tataccgcga aatccgcgat atactgcggg tcccgcgcga cccgagcaga
     1021 ataaaaagac agggaataga tggcggaaga cctgcatctg aatccactca ccggccatga
     1081 cgagttcgac cggctgatcg cggaattctc cgaattgcgc gacgccgaag caatggagtc
     1141 cgcgcggcaa ggtatctggg aacgattcgg tgtggaaggc gcggtcttca tctccgacat
     1201 ggcgagtttc tccagcacgt cgaggaagat cggggtctgc cacttcctca agctgatcca
     1261 ccgcgcgcgc cagatcatcg cgccactcgt cgaccgatat aatggcctgt tgctcaagtg
     1321 tgatgccgac aactgctacg cgttcttcga gagcaccgac gatgcgatcc gtgcaagctt
     1381 cgacatcaac gccgaactct tccggcacaa cgatgaattc agtctgagcg agcaaatcca
     1441 tttgtcgatc ggcatcgact acggccaagt cctgcttatc ggcgacaggg aattctttgg
     1501 cgatccggtg aacacggcgt cgaagcttgg tgaggatctc gcggtcaggt ccgagacact
     1561 gatcacgagg cgcgcactgg agcgtgcgac gctaatgatc ccggaaaccg cagagcacat
     1621 ggtcgctcgt atatccgaca tcgagatcag gtacgtgcgc ctgcccatga ccgagatcga
     1681 aagggacgag ctgggatgag ccgtcggctc agcgaggcac aggcgctacg cagcgtactc
     1741 gaggtcacac gcaagctcgg cgcgcctttt gatctcgaca cgatgctcac cgaagtcgtc
     1801 gatgccgccc gcagagtgct cgacgcggat cgcggcacgg tattcctgta tgacgataat
     1861 tcaaatgagt tggtcgtgcg cgttggaacc aagctcggca gcattcgcat cccggctgac
     1921 aaaggcatcg tgggcgagtc ggcgatgtcc agaaaactca ttaacgttcc ggactgttac
     1981 gaggatgaac gtttcaatcg agccatcgac aaggaaaccg ggtaccgctc ccgctgcatg
     2041 ctc
//
LOCUS       DATDAA010000249         5308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_437555,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000249 DATDAA010000000
VERSION     DATDAA010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437555"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1621
                     /locus_tag="VIS73_07855"
     CDS             <1..1621
                     /locus_tag="VIS73_07855"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5763107.1"
                     /translation="GGEPVSRFGGVIFARSDRDDLAEASDIAGRRVASATEKSFAGYL
                     VQEWALLEQGVALDTAGALIVTGMPQDRVVSAVLDGKADIGFARTGLIESLIREGRLA
                     PDALKLIAGRQVSGFSQRLSTELYPEWPFAALPHVPDATVKAVSLALLQLRADDRAAI
                     AARIYGFAPPGDYASVEALLLRLRVHPERMNLFGPAEVLQKYALELAAAAAAVLLLAI
                     AALLRMRWQNRQLAGTVDELRRLEQREELLDGLSEGVYGIDAAGHCTFVNEAACSLLG
                     YRSDQLVGRDQHAVFHHHYPDGRPYPAAECPIRRTVTDGERRSGEEIFFRHDGTPFPV
                     RYAARAIRRDGVISGAVVAFQDIGEEKAVQEKVRDLAMRDPLTGLANRRALWERLGLE
                     LARAARDGSMLAVLFVDLDGFKAINDTRGHDAGDAVLIAIAERLGASVRNVDLVGRLG
                     GDEFLVVLTGLRNAAAATAIAQKLLGAVSAPLDLEGDPSARLTASIGVAFYPEHGADV
                     STLVHRADRAMYAAKRDGKSRFVLAGEDDSD"
     gene            1696..2775
                     /gene="hemE"
                     /locus_tag="VIS73_07860"
     CDS             1696..2775
                     /gene="hemE"
                     /locus_tag="VIS73_07860"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY5763108.1"
                     /translation="MTRPKNDTFLRALRRQPTEYTPLWLMRQAGRYLPEYCETRKRAG
                     NFLNLCKSPSMACEVTLQPLSRYDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFERP
                     LKDEWEIRDLKAPDPYDHLGYVMDAVSEIRRALAGSVPLIGFSGSPWTLACYMVEGGS
                     SSDYRKVKTMAYDRPDLMHRILTVTTDAVIAYLNAQIDSGAQAVMVFDSWGGALSAAA
                     YEEFSLAYMQRIIAGLKKEKDGESVPAIVFTKGGGLWLEKIAAIGADGVGLDWTIDIG
                     EARRRTGGKIALQGNLDPNVLFAQPETVAIEAKKVLDSYYAAGGGDTGHVFNLGHGIS
                     QFTPPEHVTALVEAVHQHSRTLRQG"
     gene            3345..>5308
                     /locus_tag="VIS73_07865"
     CDS             3345..>5308
                     /locus_tag="VIS73_07865"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY5763109.1"
                     /translation="MTEPVGDIPVAAGESVISVALDVPLYRCFDYRLPEPSMDAAALV
                     GRRVCVPFGSRRQIGVVIAGPPPTGADASRELRAIESLVDDGLPPLPAEWFALCEFAA
                     TYYQTPLGEVMLQALPKGLRRPDPPKARKTRSRPAAGIAQVPPALNDEQGAAVAAIVD
                     AFGRFQPFLLFGITGSGKTEVYLHAIAAALAAGRQVLLMVPEINLTPQLEARVAARFP
                     EVPLVALHSELTEAARLRNWRAAYTGEARIVLGTRLSIFTPLPDLGLLILDEEHDASY
                     KQQDGTRYSARDLAVVRAQQRGIPIVLGSATPSLETWANAESGRYQRLLLGARAVSEA
                     RLPAVRLVDTRPEKLVDGLSAALLAAIEARLAAGEQSLVFLNRRGYAPVLACTACGWV
                     APCHRCAANLVVHRADRRLRCHHCGLETAIPRACPSCGNQDIHAFGRGTQRLEETLGE
                     RYPNARILRVDRDAARSRLQWEEFARRIHDGEADLLIGTQMLAKGHDFPKLTLVGVVG
                     ADAALFAADWRAPERLFAQLMQVAGRAGRAALPGEVLVQTEHPDHPLYRALVDHDFAA
                     FAAALLAEREQAGFPPYSFQAVLRADARQIADSLAFLGEAVALADATAHPAVAIYDPV
                     PMKLARLANRERAQLLVESPSRPALQAFLAA"
BASE COUNT          821 a         1820 c         1785 g          882 t
ORIGIN      
        1 tggcggtgag ccggtcagcc gtttcggcgg cgtgatcttc gcacgcagtg accgcgacga
       61 tttggccgaa gcctccgaca ttgccggccg gcgggtggcg agtgccaccg agaagtcctt
      121 tgctggctac ctggtgcagg aatgggcgct gctcgaacag ggtgtcgcgc tcgacacagc
      181 cggcgcgctg atcgtcaccg gcatgccgca ggatcgggtg gtgagcgccg ttctcgatgg
      241 taaggccgac atcggcttcg cccgcacggg cctaatcgag tcgttgatcc gcgaggggcg
      301 gctagcgccc gatgcactga agctcatcgc cggacggcag gtcagcgggt tttcgcagcg
      361 gctgtcgacc gagctctacc ccgagtggcc ttttgcggcc ttgccgcatg tgcccgacgc
      421 gacggtcaag gcagtttcgc tggccctgct gcagctgcgt gccgacgatc gcgcggcaat
      481 cgccgcgcgg atctatggct tcgcgccgcc cggcgactac gcttcggttg aggccctgct
      541 gctgcggctg cgggtacacc ccgaacggat gaacctgttc gggccggccg aggtgctgca
      601 gaagtacgcg cttgagttgg ccgccgccgc cgccgccgta ctgctgctgg caattgccgc
      661 gctgctcagg atgcgctggc agaaccggca gctggccgga acggtcgacg agctgcgccg
      721 gctcgagcag cgcgaggagc tgctcgacgg tctcagcgaa ggcgtatacg gaatcgacgc
      781 ggccggtcac tgcacctttg tgaacgaagc ggcctgcagc ctcctaggct atcgttccga
      841 ccagttggtc ggtcgcgacc agcacgcggt attccaccat cactatccgg atggtcgacc
      901 gtacccggcc gccgaatgtc cgatccgtcg aaccgtaacc gacggcgagc ggcgctcggg
      961 cgaggaaatc ttctttcgcc acgatggcac gccgttcccc gtgcgttacg ccgcccgagc
     1021 aatccggcgc gatggggtga tcagcggcgc cgtcgtcgcc ttccaggaca tcggcgagga
     1081 aaaggccgtg caggagaaag ttcgtgatct ggccatgcgc gatccgctta ctgggcttgc
     1141 caaccgccgc gccctgtggg agcgcctcgg ccttgaactg gcgcgcgccg cccgcgacgg
     1201 ctccatgctc gccgtgctgt tcgtcgatct cgacggcttc aaggcgatca acgacacgcg
     1261 gggccacgat gccggcgacg cagtactgat cgcgatcgcc gaacggcttg gcgcgtcggt
     1321 ccgcaacgtc gacctggtcg gacggctcgg tggcgacgag tttctcgtcg tcctcaccgg
     1381 cctgcgcaat gccgctgccg cgaccgctat cgcgcagaag ctgctcggcg ccgtcagcgc
     1441 cccgctggac ctcgaaggcg atccgtctgc acggctgacg gcgagcatcg gcgtcgcctt
     1501 ctatcccgaa cacggggccg acgtctcgac gctggtgcac cgcgccgatc gggcgatgta
     1561 cgcggccaag cgcgacggca aaagccgctt cgtgctggcc ggcgaggacg attcagactg
     1621 aagcgtggtg gggcgcgctg cgcaagcgcg cctgctgacg gataatccgg cctttgcttt
     1681 ccgccggaga caggcatgac ccgccccaag aacgacactt tcctgcgcgc actgcgccgc
     1741 cagccgaccg agtacacgcc gctgtggctg atgcggcagg cgggtcgcta tctccccgag
     1801 tactgcgaga cgcgcaagcg tgcgggtaat ttcctcaacc tctgcaagag cccgtcgatg
     1861 gcctgcgagg tgacgctgca gccgctgtcg cgctacgacc tcgacgcggc gattctgttc
     1921 tccgacatcc tcacggtgcc agacgcaatg ggattgggcc tttatttcgc cgaaggcgag
     1981 gggccgaagt tcgagcggcc gctcaaggac gagtgggaaa tccgcgacct caaggcgccc
     2041 gatccctacg accaccttgg ctatgtcatg gacgcggtca gcgagatccg ccgggcgctg
     2101 gccggctcgg taccgctgat cggcttttcc ggcagcccgt ggacgctggc ctgctacatg
     2161 gtcgaaggcg gctcctcgtc ggattaccga aaggtcaaga ctatggccta cgaccggccc
     2221 gatctgatgc accgcatcct gaccgtgacc accgatgcgg tgatcgccta ccttaacgcc
     2281 cagatcgact cgggcgcgca ggcggtgatg gtcttcgact cctggggtgg cgcgctgtcg
     2341 gcggcggcct acgaggaatt ttcgctcgcc tacatgcagc gcatcattgc cggcctgaag
     2401 aaagaaaagg acggggagtc ggtgccggcc atcgtcttca ccaagggcgg cgggctctgg
     2461 ctcgagaaga tcgccgcgat cggcgccgac ggggtcggcc tcgactggac catcgacatc
     2521 ggcgaagcgc gccggcgtac cggcggcaag atcgcgctgc agggcaacct cgaccccaac
     2581 gtcctgttcg cgcagcccga gaccgtcgcc atcgaggcga agaaggtgct cgacagctac
     2641 tacgcggccg gcggtggcga tactgggcac gtcttcaacc tcggccacgg catatcgcag
     2701 ttcaccccgc cagaacacgt cacggcgctg gtcgaggccg tgcaccagca cagccggacc
     2761 ctgcggcagg gctgagcgcg gctggccgcg caggcgagcg cgccggtgcc cgatcagcgc
     2821 tgcggcggtg aggcggagca ccgcgctgca gtagcgccgc ggaccgccgc actttcccgg
     2881 cgggctgccg tcccgtcgcc tgcggtgtga gccgccgctt cgtgatttct gccgactggg
     2941 tgggccgttc ggaacaggtg cagttgcgtg ttgacatagc tcatgaaaac acgcttaaga
     3001 acctacttat gcacagttgc cgctcttgcc gcagcgcgtc aagagcggtg gccgtccggc
     3061 cctgttgatt cgtggcatct atatgttttc aatagataaa aaccctcaag ccggccgcca
     3121 agccagaaaa tcgcattgtt tgtcaagggc ttgccggcga aaatcgcccg cgcaactcac
     3181 agacttatcc acaagctggc cgagcgcggc aaaatccttt gtcactgaga tgcttagcgg
     3241 taatttccaa gaattctcag aagatctggc gctaagctca tgttcgaaag gcgataggcg
     3301 cagttcagac ctggccaaag gtcataggcg ccggcggttg cggcatgact gagccagtgg
     3361 gcgacatccc ggtggcagct ggcgaatccg tgatctcagt cgcactcgac gtaccgctct
     3421 accgatgctt cgattaccgc ctccccgagc cgtcgatgga cgcggcggcg ctggtcggcc
     3481 ggcgggtctg tgttcccttt ggcagccgcc ggcagatcgg cgtggtcatc gccgggccgc
     3541 cgccgacggg ggctgatgcg tcgcgcgagc tgcgggccat cgagtcgctg gtcgacgatg
     3601 gcctgccgcc cttgccggcc gagtggttcg cgctctgcga gttcgccgcc acgtattacc
     3661 agaccccgct cggcgaggtg atgctgcagg cgctgcccaa gggcctgcgc cgacccgatc
     3721 cgccaaaagc gcgcaagaca cgtagtcggc cggcagccgg cattgctcag gttcccccgg
     3781 cgcttaacga cgagcagggc gccgcggtcg ccgccatcgt tgatgccttc ggccgctttc
     3841 agcccttcct gctcttcggc atcaccggca gcggcaagac cgaggtctat ctgcacgcga
     3901 tcgccgcggc gctggccgct gggcggcagg tgctgctgat ggtgcccgag atcaatctca
     3961 cgccgcagct agaagcgcgc gtcgctgcgc gttttcccga ggtgccgctg gtcgcgctgc
     4021 acagcgagct gaccgaggcg gcccggctgc gcaactggcg cgcggcctac accggcgagg
     4081 cgcggatcgt cctcggcacc cggctgtcga tcttcacgcc gctgcccgat ctcggactgc
     4141 tgatcctcga cgaggagcac gatgcctcgt acaaacagca ggacggcacg cgctattcgg
     4201 cgcgcgacct ggccgtggtg cgggcccagc agcgcggcat cccgatcgtg ctcggctcgg
     4261 cgacgccgtc gctggagacc tgggccaacg ccgagagcgg ccgctaccag cgcctgctgc
     4321 tcggcgcccg tgccgtttcc gaggcgcgcc tgccggccgt ccgcctggtc gacacgcgcc
     4381 cggagaagct cgtcgatgga ctgtcggcgg cgctgctggc ggcgatcgag gcgcgcctgg
     4441 cggccggcga gcagtcgctg gtcttcctca accggcgggg ctacgcgccg gtactcgcgt
     4501 gcaccgcttg cgggtgggtg gcgccctgcc accgctgcgc ggcgaacctg gtcgtgcacc
     4561 gcgccgaccg ccgcctgcgc tgccaccatt gcggactgga aacggcaatc ccgcgcgcct
     4621 gcccaagttg cggcaatcag gacatccacg ccttcgggcg cggcacccag cgactcgaag
     4681 agacgctggg cgagcgctac ccgaacgcgc gaatcctccg tgtagaccgc gacgcggcgc
     4741 gctcgcgcct tcagtgggag gagttcgcgc ggcgcatcca cgacggcgag gcagacctgc
     4801 tgatcggcac gcagatgctc gccaagggcc acgactttcc caagctcacg ctggtcggcg
     4861 tggtcggcgc cgatgccgcg ctgttcgccg ccgactggcg ggcgcccgaa cgtctcttcg
     4921 cgcagctcat gcaggtggct ggtcgtgccg gccgcgctgc gctgcccggc gaggtgctgg
     4981 tgcagaccga gcacccggat cacccgctct accgtgcgct ggtcgatcac gacttcgcgg
     5041 ccttcgccgc ggcgctgctt gccgagcgcg agcaggccgg gtttccgccc tacagctttc
     5101 aggccgtgct gcgcgccgac gctcggcaga tcgccgattc gctggccttt ctcggcgaag
     5161 cggtagcgct cgccgacgca acggcacatc cggcggtggc aatctacgac ccggtgccga
     5221 tgaagctcgc ccgcctggcc aaccgcgagc gcgcgcagct gctcgtcgaa tcgccgtcgc
     5281 gaccagcact gcaggccttt ctcgccgc
//
LOCUS       DATDAA010000250         6694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_87926,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000250 DATDAA010000000
VERSION     DATDAA010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87926"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..1506
                     /gene="glp"
                     /locus_tag="VIS73_07870"
     CDS             301..1506
                     /gene="glp"
                     /locus_tag="VIS73_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY5763110.1"
                     /translation="MPLLSFADAKSALLAAARPIPESETIDLADASGRVLADDLRSPI
                     DVPGFDNTAMDGYAVRAADVASAGTRLPVSQRIAAGSFPEALPPASAARIFTGAPLPA
                     GADAVVMQEDCREADGIVTIDAPPPTGQHVRRAGSDIARDSIALPAGTRLSAAALGLA
                     SSIGIARVRVRRRLKVAIFFTGSELVMPGTPLAAGQIYNSNRYVLRGFLREAGAEVID
                     LGIVRDDLAATRAALREAAHEADLILTSGGMSEGEEDHVKPAVEAEGRIDVWKIAAKP
                     GKPLAFGDVTSGGRSVAFVGLPGNPVSVWAGLLTLVRPFLRAMEGDRELEPPLLKLRA
                     DFDYTVKGNRLEFVRVRRNTDGGLDIFRTQDSAVLSSAVWADGVAPLPAGTTVRPGDL
                     VDYLPGPPR"
     gene            1525..1917
                     /locus_tag="VIS73_07875"
     CDS             1525..1917
                     /locus_tag="VIS73_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763111.1"
                     /translation="MSAHSAIRVSLARRWASVLLRAAHLVAVIGLAAEIVSATTASST
                     HAHLWPAAVLVTGLALFTVDFWARPGRLLELLATSIAAKLLLVALMIVLPTWKTPLFW
                     VIIVWSVIFSHAPATFRHARIWRRRAPR"
     gene            complement(1949..2185)
                     /locus_tag="VIS73_07880"
     CDS             complement(1949..2185)
                     /locus_tag="VIS73_07880"
                     /inference="COORDINATES: protein motif:HMM:NF023513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 family protein"
                     /protein_id="HEY5763112.1"
                     /translation="MNGCIYESERYAIVAFPRYDAMELIDKQSSRNAFVVGAEARGLH
                     HSIIQIPEDERTTEVIDELLDDFCQDRVQPMAIH"
     gene            2335..3783
                     /locus_tag="VIS73_07885"
     CDS             2335..3783
                     /locus_tag="VIS73_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5763113.1"
                     /translation="MPETTSNKSPSSAMSLAAACAKLPPLLEGLEAGLVERRRVVRMA
                     LLVALSGEHTLLIGPPGTAKSELARRLHHAFTGGRYFERLLTRFSVPEELFGPLSISA
                     LEDDRYERQTAGFLPEASIAFIDEVFKANSAILNALLTLLNEREFDNGAGRQRCPLIS
                     VIGATNAVPADEVGEAFFDRFLARIPVEPVSSTGFAALLARDGEADIPPLLPLDEAER
                     AALSAAARSVELPEAVVAILADLRETLAEAAPVSDRRWVKLVRLLKTAAASEGRAAVS
                     IWDLALLPACVAADATGQAAVRDWLATRLGVREAFSPPRLTRIVEAFEAQLEIEQNAE
                     DLEYDANGRLQFTAGDENSLADRVGDAKGGAAAPRLSYSRKRRYGEAHIGARTAQLDS
                     LVERIRAYVAELDSVRDQLAQHLATSLWLDETFAEPAAANLAATAVMLQTLLARSAAA
                     RAGFTALPRLPNAGELPEPVAHEALPAIGRAA"
     gene            3780..5396
                     /locus_tag="VIS73_07890"
     CDS             3780..5396
                     /locus_tag="VIS73_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5763114.1"
                     /translation="MSTVLDRREGTAIPREVRGVTAAEARAQAEALVFGRLEARLAPL
                     EALARGLWLATLTNSQGGLETRLPGISTLHASLAAGLTDHGDWLDPALATRIVETLDS
                     LALPAFCADKPELAEQVLRSLLWHLDRIVDYVDRGNDQAAARDKALADFAGDWQERCN
                     LIDELATVFGEMGDLDKHTRWDLLRGALKSGAWQEVLRVRALIEHLPELVRVIRRLGR
                     ARPAEQQSETMARAIETQHLIAAPRAARRTTRVPDLPGETRGITRAGRIARMLPSESM
                     LLAHPRLRLIWHARHAERTLLCYDDDDRLEDSHDVRLPALAPSPQRRPDRPPEAGPML
                     VCVDTSASMRGGAEAVAKAVVLEAARTAFAEQRACQVLAFGGTDELIEFTVSRDVAGV
                     TRLAEFLGQSFAGGTDICAPLERALERVRADGWRNADLLIASDGEFGATPDKAEALRN
                     AKGELGLRVQGVLIGDRETIGFLEVADDILWVRDWRRFGDSTADSPVHSQRLTAIYFP
                     GSLRGASAADAPPPDPAEAFRRPLAVRGKS"
     gene            5393..6229
                     /locus_tag="VIS73_07895"
     CDS             5393..6229
                     /locus_tag="VIS73_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HEY5763115.1"
                     /translation="MSSTVPQELIALLESFAARAPLPRVRTLHLPPASAAGSKDGEFC
                     ALELADGSIGLSYVLLADTLHQLIAGAAGALSGADPLRLARDFAEQSGARRAAGFAAV
                     NALTRCLFDRAGYTPALDVDSIGSLEPQAGDHVGMIGFFRPLVGRFVDAGARLTVAEL
                     NPEFAGDFDGYRVTLDPAELAGCNKVLSTSTVLLNDTLDDMLGHCRGATRFVMVGPGA
                     GCLPDPLFARGVTLLGGAWITDGPELIERVRAGAKWSEATRKSAIQRDGYPGIAALLQ
                     RV"
     gene            6320..>6694
                     /locus_tag="VIS73_07900"
     CDS             6320..>6694
                     /locus_tag="VIS73_07900"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY5763116.1"
                     /translation="MMTQPLRNLLCALTLFPALALAALPTPSPTVRYLSLGDSIAAGY
                     KAQPATAGYAYQLYLGEAYGRTPDTVFANVAMPGATSTDVLTFQLPQVPRFVPTVVTM
                     SVGGNDLLTLLASAPPSPEQVSA"
BASE COUNT         1027 a         2432 c         2136 g         1099 t
ORIGIN      
        1 aggaatccgc cgagtcctaa ccccaatttc ggcgttgcga atccagcttc ttctgccgcc
       61 gccggggtca tggacaccca gaatgccaag aacaccgtcc agtaccgtat tcgcactgtc
      121 catctccgcc cacaccaacg atcccccaga ttaggatgcc gcgacagcag gatcaatcgc
      181 caaatagcgt ccgccgttcc gtcctacagc acacttggtc gataatgcgg taccgcgtcg
      241 gaacactctg gacgacagcc ctgctgcgcc gggcgcacca atcaccgtag aagaccgaac
      301 atgcccctgc tttccttcgc cgacgccaag tcggcactac tcgccgccgc acggccgatt
      361 ccggagtcgg agacgatcga cctcgccgac gcctcgggcc gcgtcctcgc cgatgacctc
      421 cgctcgccga tcgacgtacc cggcttcgac aacacggcga tggacggcta cgcggtgcgt
      481 gccgccgacg tggccagtgc cggcacccgg ctgccggtca gccagcgcat cgccgccggc
      541 agctttcccg aagcgctgcc cccggcaagc gccgcgcgca tcttcaccgg tgcgccgctg
      601 cccgccggcg ccgacgccgt ggtgatgcag gaggactgcc gcgaagccga cgggatcgtc
      661 accatcgatg ctccgccacc aaccgggcaa cacgtccgcc gcgccggctc ggacatcgcc
      721 cgtgacagca tcgccctgcc agccggaacg cgtctttccg cagcggcgct gggactcgcc
      781 tcgtcgatcg gcatcgcccg cgttcgcgtg cgccggcggc tgaaagtcgc gatcttcttc
      841 accggcagcg aactggtcat gcccggcacg ccgctcgccg ccgggcagat ctacaactcg
      901 aaccgctacg tgttgcgcgg atttctgcgg gaagcgggcg ccgaggtgat cgacctcggc
      961 atcgtccgcg acgatctcgc ggcgacgcgc gcagccctgc gcgaggcagc gcacgaagcc
     1021 gacctgattc tcaccagcgg cggcatgtcg gagggtgagg aagatcacgt caagccggcc
     1081 gtcgaagccg aaggccgcat tgacgtctgg aagatcgctg ccaagccggg caagccgctc
     1141 gctttcggcg acgtgaccag cggcggccgc tcggtcgcct ttgtcggcct gcccggcaac
     1201 ccggtttcgg tgtgggcagg gctgctcacc ctggtacggc ccttcctgcg cgccatggaa
     1261 ggcgaccgcg aactcgagcc gccgctcctc aagctgcgcg ccgatttcga ctacacggtg
     1321 aaaggcaatc ggctcgaatt cgtccgcgtg cggcgcaata ccgacggcgg gctcgacatc
     1381 ttccgcacgc aggactcggc tgtgctctcg tcggccgtat gggccgacgg tgtcgcacca
     1441 ctgccggccg gcaccaccgt ccgcccgggg gatctggtcg attatctgcc cggtccgccg
     1501 cgctgagccg cagcgaacag acccatgtcc gcgcactccg ccatccgagt ttcgctggcc
     1561 cgccggtggg ccagtgtctt gctgcgcgca gcgcacctgg ttgcggtcat cggtctcgcg
     1621 gccgagatcg tcagcgccac tacagcctcc tcgacgcacg cgcacctatg gccagccgcg
     1681 gtgctggtca ccggcctcgc attattcacg gtcgatttct gggcccgtcc gggccgcttg
     1741 cttgaactgc tcgccaccag catcgccgcc aagttgctgc tggtggcctt gatgatcgtg
     1801 ctgccgacat ggaagactcc gctgttctgg gtcatcatcg tctggtcggt gatcttctcg
     1861 catgcgccag cgacctttcg ccacgcgcgc atctggcgac gccgggcgcc gcgctagccg
     1921 cgcccgacct tgcccgctgg ttcggccgtc agtgaatggc catcggctgg acgcgatcct
     1981 gacagaagtc atcgagcagt tcgtcaatga cctcggtcgt gcgctcgtcc tcggggatct
     2041 ggatgatcga atggtgcagc ccacgcgctt cggcgccgac gacgaaggcg ttgcgactgg
     2101 actgcttgtc gatgagctcc atcgcgtcgt agcgcggaaa ggcaacgatc gcgtagcgct
     2161 cactctcata tatgcagccg ttcatctcac acctccttcg aaaaagccca caactccgat
     2221 atggcaccgg ttgcccgctt ttcaataaga ccccgcgccg tccggaacgt ttcctgctat
     2281 atcgacattg ccgaaatcag tgaaatgacc ctcgccgcaa cgtaggatca agaaatgccc
     2341 gagacaacct ccaacaagtc gccttcgtcc gccatgtcgc tcgccgccgc ctgcgccaag
     2401 ctgccgccct tgctggaagg cctggaagcc ggcctcgttg agcgccgacg ggtggtgcgc
     2461 atggcgctgc tggtggcgct gtccggtgag cacacgctgc tgatcggtcc gccgggcacc
     2521 gccaagagtg agttggcgcg tcgcctgcac cacgccttca ccggcggccg ctacttcgag
     2581 aggctgctga cgcgcttctc ggttcccgaa gagcttttcg ggccgctgtc gatcagcgcg
     2641 ctcgaggacg accgctacga gcggcagacc gccggattcc tccccgaggc gtcgatcgcc
     2701 ttcatcgacg aggtgttcaa ggccaacagt gcgatcctca atgcgctctt gacgctgctc
     2761 aacgagcgcg aattcgacaa cggcgccggc cgccagcgct gcccactgat cagcgtcatc
     2821 ggtgcgacca atgcggtacc ggccgacgag gtcggcgaag ccttctttga ccgcttcctc
     2881 gcccgcattc cggtcgagcc ggtgagcagc accggcttcg ccgccctgct cgcacgtgat
     2941 ggcgaggcag atatcccacc gctgctgcca ctcgacgagg cagagcgagc agcactgagc
     3001 gcagccgcac gctcggtgga gctaccggaa gcggtggtcg caattctcgc cgacttgcgg
     3061 gaaacgctgg ccgaggcagc gccggtttcc gaccgtcgct gggtcaagct ggtgcgcctg
     3121 ctgaaaaccg cggcggccag cgaaggccgc gcggcagtat cgatctggga tctggcgctg
     3181 ctgccggcct gcgtcgccgc cgacgccacc gggcaagcgg ccgtgcgcga ctggctggcc
     3241 acccgcctcg gcgtgcgcga agccttttcg ccgccgcgac tgacgcggat cgtcgaggct
     3301 ttcgaggcgc aactcgagat cgaacagaac gccgaggatc tcgaatacga cgccaacggt
     3361 cgactgcaat tcaccgccgg cgacgagaac tcgctggccg accgggtcgg cgatgccaag
     3421 ggtggcgccg ccgcgccgcg cctcagctat agccgcaagc gtcgttacgg cgaggcgcac
     3481 atcggcgcgc ggaccgcgca gctcgattcg ctggtcgagc ggattcgcgc ttacgtcgcc
     3541 gaactcgatt ccgttcgcga ccagttggcg cagcatctcg ccacgagtct ctggctcgac
     3601 gaaacctttg ccgagccggc ggcggcgaac cttgcggcga ccgccgtcat gctgcaaacg
     3661 ctgctcgccc gcagcgccgc cgcgcgggcc ggctttacgg cgctgccgcg cctgccgaac
     3721 gccggcgagt tgcccgaacc ggtcgcacac gaagcgctgc cggcgattgg ccgggcggcg
     3781 tgagtaccgt actcgatcga cgcgagggca cggcaatccc gcgcgaggtc cgcggcgtca
     3841 ctgccgctga agcgcgcgcg caggccgagg cgctggtgtt tggccggctc gaggcgcggc
     3901 tcgctccgct cgaggcgctc gcccgcggcc tctggctggc gaccctgact aattcgcagg
     3961 gcggactgga aacgcgcctg ccgggcatct cgacgctgca cgcgtcgctc gccgccggtc
     4021 tgaccgatca cggcgactgg ctcgatccag ccctggccac gcgcatcgtc gagacgctcg
     4081 attcgcttgc gctgccggcc ttctgcgccg acaagccgga actggccgaa caggtgttgc
     4141 gctcgctgct ctggcatctc gatcgtatcg tcgactatgt cgaccgcggc aacgaccaag
     4201 ccgccgcgcg agacaaggca ctcgccgatt tcgccggcga ctggcaggaa cgctgcaacc
     4261 tgatcgatga actcgccacc gttttcggcg agatgggcga cctcgacaag cacacgcgct
     4321 gggatctgct gcgcggcgcg ctgaagagcg gtgcctggca ggaagtcctg cgtgtccgcg
     4381 cgctgatcga acacctgccc gaactcgtgc gcgtgattcg ccggctcggc cgtgcgcgtc
     4441 ctgccgagca gcagtccgag accatggccc gcgccatcga aacgcagcac ctgatcgccg
     4501 ccccgcgtgc tgcccggcgg accacccgcg tccccgatct gccgggggag acacgtggca
     4561 tcacccgcgc cggacgcatc gcgcggatgc tgccatccga atcaatgctg ctcgcccacc
     4621 cgcgcctgcg gctgatctgg cacgcgcgcc acgcagagcg gacgctgctc tgctacgacg
     4681 acgacgatcg cctcgaggat agccacgatg tccgcctgcc ggcgctggca ccgagcccgc
     4741 agcggcgtcc cgaccgacca cccgaagccg ggccgatgct ggtctgcgtc gatacctcgg
     4801 cgtcgatgcg cggcggcgcc gaggcggtgg ccaaggccgt cgtcctcgaa gccgcgcgca
     4861 ctgcctttgc cgagcagcgc gcctgccagg tgctggcctt cggcggcacc gacgaactga
     4921 tcgagttcac ggtcagccgc gacgtcgccg gcgtcacccg actggccgaa ttccttggcc
     4981 agagtttcgc tggcggcacc gacatctgcg cgccgctcga gcgtgcgctc gagcgcgtca
     5041 gggccgacgg ctggcgcaac gccgacctgc tgatcgcctc ggacggcgag ttcggcgcga
     5101 cgcccgacaa ggccgaggcc ctgcgcaacg ccaagggcga gcttggcctg cgtgtccagg
     5161 gcgtgctcat cggtgaccgc gaaaccatcg gtttcctcga agtcgccgac gacattctct
     5221 gggtccgtga ctggcggcgc ttcggcgact ccactgccga ttcgcccgtg cattcgcagc
     5281 ggctgacggc gatctacttc cccggctccc tgcgcggtgc gagcgcggcc gacgcgccgc
     5341 cgcccgatcc cgccgaggca ttccggagac cactggctgt aaggggaaag tcatgagcag
     5401 cacggttccg caggaactga tcgcgctgct cgaatccttc gctgcccgag caccgctgcc
     5461 gcgcgtgcgg acactgcacc taccacctgc gtcggccgcc ggcagcaagg acggcgagtt
     5521 ctgtgcactc gaactggctg acggcagcat cgggctttcc tacgtcctgc tcgccgacac
     5581 gctgcatcag ctgatcgccg gcgcggccgg cgcgctctct ggtgccgatc cgctgcggct
     5641 ggcacgcgat tttgccgagc aatccggcgc ccgccgcgcg gccggttttg ccgccgtcaa
     5701 tgccctgacg cgttgcctgt tcgaccgcgc cggctacacg ccagcactgg acgtcgattc
     5761 gatcggcagc ctcgagcctc aggccggcga ccacgtcggc atgatcggct tttttcgccc
     5821 actggtcggc cgcttcgtcg acgctggcgc gcgcctgacc gttgccgagc tgaaccctga
     5881 gtttgccggt gacttcgacg gctaccgcgt caccctcgac cccgccgaac tcgctggctg
     5941 caacaaggtg ctctcgacca gcacggtgct gctcaacgac acgctcgacg acatgcttgg
     6001 ccactgccgc ggcgccaccc ggttcgtcat ggtcggcccc ggtgccggct gcctgcccga
     6061 cccgctgttc gcgcgcgggg tgacgctgct cggcggcgcc tggatcactg acgggccgga
     6121 actgattgag cgggtacgcg ccggtgcgaa gtggagcgag gcgacgcgca agtcggcgat
     6181 ccagcgtgac ggctacccgg gcatcgcggc gttgctgcaa cgcgtttgaa acgctgctgt
     6241 cttgaacttg tctccgcgct ggctactctg ttcctgtagc ggcgcgttcg cgtcgccttc
     6301 cgcaatccgg gggtaagcca tgatgaccca accgctgcgc aaccttcttt gcgcgctcac
     6361 cctgttcccg gcgctagcgc tcgccgccct gcccacaccg agcccgacgg tccgctatct
     6421 ttcgctcggc gatagcatcg ccgccggcta caaggcgcag ccggcaacgg caggctacgc
     6481 ctaccagctc tatcttgggg aggcgtacgg gcgtacgccc gacacggtgt ttgccaacgt
     6541 cgcgatgccc ggcgcgacga gtaccgacgt actcaccttc cagttgccgc aggtgccgcg
     6601 cttcgtgccg accgtggtga ccatgtcggt cggcggcaac gacctgctga cgctgctcgc
     6661 cagcgcgccg ccctcgccag aacaggtcag cgcg
//
LOCUS       DATDAA010000251        11720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_18046,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000251 DATDAA010000000
VERSION     DATDAA010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11720
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18046"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="VIS73_07905"
     CDS             complement(<1..1545)
                     /locus_tag="VIS73_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEY5763117.1"
                     /translation="MLLAALLVPSAATAQPAPEGESGFTRKPSVFAEKAMVVAAHPAA
                     SAAGLRILDAGGSAVDAAIAAQLVLAVVEPQSSGIGGGGFLLSFDGQRVRAYDGRETA
                     PATADDALFLDAGGKPLPFFDAVVGGRSVGVPGLVRMLELAHRRDGRLSWARLFDDAI
                     ALSERGFPVSPRLHKLLADDRYLRRDPAARALYYESDGRAKAVGNVLRNPELAATLQQ
                     LATEGADALHSGELAAAIVAAVNGHPSNPGRMTAADLSGYRALERAPLCTGFLAWRVC
                     GMPPPSSGGLAVAQILGILAAGDMTAFTAPGSAPSVDAIHRFSEAGRLAFADRNRYVA
                     DPDFVPLPAGLHDNGYLRQRAALIGTHSMGSATAGNPGAVPLGWIDGHDHEVPATTHL
                     SVVDAAGHAVALTSSIEHAFGARLMVRGFLLNNQLTDFSFAPRDADGLVANRVGGGKR
                     PRSSMSPTLAFDRESGRLELITGSPGGPFIIGYVASTLVGMLVGQQAPDETLAQPHFG
                     SRNGPTE"
     gene            complement(1592..3196)
                     /locus_tag="VIS73_07910"
     CDS             complement(1592..3196)
                     /locus_tag="VIS73_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEY5763118.1"
                     /translation="MIDSPFGPEVKRRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAG
                     TVKAKKSDRHATSDWMEVEKQRGISVASSVMQFEFDDCVINLLDTPGHEDFSEDTYRV
                     LTAVDCALMVIDAAKGVEERTIKLMEVCRMRDTPIVTFINKLDREVRESIELLDEIEK
                     VLDISCAPITWPIGMGKGFKGVLNLKSHVVLKFAPGQERGDQGFEKVTGAHLEDLAHK
                     LGVSPDSFIDDVELVGAACPDFNHAEFLAGRQSPVFFGSAINNFGVREVLQALVDWAP
                     PPQPRDATKRAVDAVETPFSGFVFKIQANMDPNHRDRIAFLRVCSGRYEPGIKVKNQR
                     VGKEIRIQNAVTFLANTRARSEEAFAGDIIGIHNHGQLQIGDTLADEDGLSFTGIPYF
                     APELFRSPRLRDPLKAKQLYKGLKELGEEGAVQVFEPYGGGNLLIGAVGPLQFEIVAA
                     RLKSEYSVEAVFEASPIVAARWVTCDDPAQWREFENSQAARLAKDVDDNWVYLVANRV
                     YLQTTGERFPKVKFHASREHGKKLSG"
     gene            complement(3193..4023)
                     /gene="pyrF"
                     /locus_tag="VIS73_07915"
     CDS             complement(3193..4023)
                     /gene="pyrF"
                     /locus_tag="VIS73_07915"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY5763119.1"
                     /translation="MHFMSSLASAWRDRNTLLCVGLDPDPKKSPAHLAGRPDAILAFC
                     REIVDATADLVCCFKPQIAYFAAQRAEDQLEALIAHIHEQHPGTPVILDAKRGDIGST
                     AEQYAIEAFERFRADAITVNPYMGRDSVEPYLAYPDKGVILLCRTSNPGGSDLQFLDV
                     GGEKLYERVARLVASEWNASGNCALVVGATFPTEIARVREITGEMPLLVPGIGAQGGD
                     IEATLKAGQTAGGGGLMINSSRAILYAGKGEDFAAKAREAAIATRDAINAHRHGGAPR
                     "
     gene            4168..4608
                     /gene="trxC"
                     /locus_tag="VIS73_07920"
     CDS             4168..4608
                     /gene="trxC"
                     /locus_tag="VIS73_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HEY5763120.1"
                     /translation="MSDPLHLVCPHCHAVNRIPAARLGDQPACGKCHQPVFSGHPTTL
                     GGSDFDTHVNRSDVPVLVDFWAEWCGPCKMMAPQFARAAAQLEPQVRLAKVDTEAEQA
                     LAARYGIRSIPTMVLFRDGREIARQSGAMSAEDITRWTRQALGA"
     gene            complement(4630..6036)
                     /locus_tag="VIS73_07925"
     CDS             complement(4630..6036)
                     /locus_tag="VIS73_07925"
                     /inference="COORDINATES: protein motif:HMM:NF021593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HEY5763121.1"
                     /translation="MMAARTSALTQGIQALAGMPAGDGMPIPGDPARLAALAPAATSK
                     GAARTKLWTLDRKFHCLTIGTCIPVDELAKVARRFAFAAKPCDDFGIHVEAVGYCGAR
                     NAVSEALQRLLDRLYAAALRDFAALRNAEQLRERWDRCFDRGQVAGPLWAALSHKHAD
                     QALRQALYAQVHMLSHQIGAGLAADVRRLEFLERDASDARRELAETRRTLAQRAEIGS
                     AELASLRAENAALRGDRDALQAAQARLNQIESGVAMTGMARRLLQLSDENAALRTVAA
                     RCDGLAERAYRLQRELAASRRETTALRAEREALNGILTAALKPQKPAASAAGGTVPDR
                     DDCLRCAEQNGLSRSRCILCVGGRTSLLSHYRALAERLGIRLLHHDGGQEEALSRLPE
                     MIASADAVLCPTDCVGHAAYYSLKQQCRKTGKRCLLFKGAGVTSFAFALSRLAAGESS
                     LGAGTGETTETEASGLAS"
     gene            complement(6033..6314)
                     /locus_tag="VIS73_07930"
     CDS             complement(6033..6314)
                     /locus_tag="VIS73_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763122.1"
                     /translation="MHALAPASTLALAPAASPQTLRQPATPTPFAARASLFAAALSEL
                     LRHQLSGCRHAAWKAADLLERLTCLPGLDDETRELCDRMSERLARVDGA"
     gene            complement(6438..6914)
                     /gene="bfr"
                     /locus_tag="VIS73_07935"
     CDS             complement(6438..6914)
                     /gene="bfr"
                     /locus_tag="VIS73_07935"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEY5763123.1"
                     /translation="MKGDKKVIQMLNKCLVNELTAINQYFLHARMYKNWGFNALGKHE
                     YEESIEEMHHADKLIERVLFLEGLPNLQALNKLLIGENVPECIAGDLKLETGAHADTK
                     AAITYCESINDYVSRAILQEILDDTEEHIDYLETQLELIDKVGIHNYLQTQIGEAS"
     gene            complement(6927..7136)
                     /locus_tag="VIS73_07940"
     CDS             complement(6927..7136)
                     /locus_tag="VIS73_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY5763124.1"
                     /translation="MYVCVCNAVTESDIDNAVAAGCTTLRELRESLGVGTCCGRCSDC
                     ARRRLHQTATPATKRQRPVCQLAAA"
     gene            complement(7148..7384)
                     /locus_tag="VIS73_07945"
     CDS             complement(7148..7384)
                     /locus_tag="VIS73_07945"
                     /inference="COORDINATES: protein motif:HMM:NF022103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HEY5763125.1"
                     /translation="MLLICKVAGEAMAHPTSKATSAWPSSQRGRDSLPWAGPETLSVD
                     SQSLLGQRSELTIRHRDETYRLRETRLGKLILTK"
     gene            complement(7450..8781)
                     /locus_tag="VIS73_07950"
     CDS             complement(7450..8781)
                     /locus_tag="VIS73_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEY5763126.1"
                     /translation="MLTIVVPVLNEAARIEATLEGLQPLRARGAELIVVDGDSDDDTA
                     ALAAPLADYVVGSRRGRARQMNAGTERANGATLLFLHADTTLPADADQHIAGALRRGA
                     RWGRFDVQIIGRHPLLPLVAWLMNQRSRLTGIATGDQAIFVDRATFVAAGGFPDQALM
                     EDIALSTKLRRHSRPACLPGPAQTSGRRWDEHGFWRTIGLMWRLRLAYWRGADPAALA
                     RRYGYATDDTASPGPLARRVRRSSGRRVALLVFAKAPVPGAVKTRLIPAIGADAAAAL
                     AVRLLERTLTTAAAGRFASLQLWCTPDARHPALVVAATRHGASMHVQNGADLGERMQT
                     ALAEALTDHHAALLIGTDCPELSVDDLRNAAQQLAGPRDVALVPAADGGYVLIGLTRP
                     SAAVFAGVDWGSPRVMDQTRARAAAAGLRLHFGRTLRDIDHPEDLPLAEFS"
     gene            complement(8823..9635)
                     /locus_tag="VIS73_07955"
     CDS             complement(8823..9635)
                     /locus_tag="VIS73_07955"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEY5763127.1"
                     /translation="MKRVLIVTLLLLASSAAVAFDNNHAAWNALLKQYVVVAPDGNAS
                     AVRYAAVAKDRANLRAYLDQLSSVTADEFAGWSKPQRLAFLINAYNAYTVELILTRYP
                     KLDSIKDIGGWFGSPWKQRFFKLLGEERHLDEIEHGIIRAPGAYDEPRIHVAVVCASI
                     GCPMLRNTAFTADTLDADLADAMRRFLADQSRNRYDARSNTLQVSKIFDWYGDDFARQ
                     GDRRKGLLQLFAAHAAELAPQAADRETLLAAITADTVTIRHLDYDWLLNDAR"
     gene            complement(9632..>11720)
                     /locus_tag="VIS73_07960"
     CDS             complement(9632..>11720)
                     /locus_tag="VIS73_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5763128.1"
                     /translation="DYLKSQQEAITMYRDANPLSTAAIYFVAYVAATGLSLPGAAVLT
                     LAGGAIFGLLWGTVIVSFASTLGATLAFLAARFVLRDTVRAKFGDKLKAIDAGIAKDG
                     PFYLFTLRLVPVFPFFAINLAMGLTPMRAITFAWVSQLGMFAGTIVYVNAGTQLAQID
                     SAAGLLSPGLLGAFALLGIFPLIAKKIVEWIKARKIYAGWQRPVHFDRNLVVIGAGSA
                     GLVTAYIAAAVKAKVTLVEKHKMGGDCLNTGCVPSKALIRSAKFLSHVSRSREFGIRE
                     SSAAFDFAEVMERVQAVVQAVEPHDSVERYTGLGVEVVEGTAKIVSPWSVEITRSDGK
                     AKTLSTRSIVIAAGARPFVPPIPGLDEVGYLTSDTVWSLRELPKRLVVLGGGPIGSEL
                     TQAFARFGAEVTQVEMGERIMAREDSEVSELVAARFRAEGIDLRLSHQAKRFLVDNGE
                     KILVAEHAGAEVRIPFDAVLVAVGRAANLKGYGLEELGIPVGRTVEVNEYLQTKFPNI
                     YAAGDVAGPYQFTHTAAHQAWYAAVNALFDPLKKFRADYSVIPWATFVEPEVARVGIN
                     ELEARERNIAYEVTHYGIDDLDRAIADGEAHGFVKVLTVPGKDRILGVTLVGEHAGDL
                     IAEFVLAMRHGIGLNKLLGTIHIYPTLAEANKYAAGNWKKAHAPQGLLRWVERFHGWR
                     RGSPANAAAGAAR"
BASE COUNT         1820 a         3740 c         4159 g         2001 t
ORIGIN      
        1 ttcggttggg ccgttgcggc tgccgaagtg cggctgtgcg agcgtttcgt cgggcgcctg
       61 ttggccgacc agcatcccca ccagcgtgct tgcgacgtag ccgatgatga aagggccgcc
      121 cggcgagccg gtgatgagtt ccaggcggcc cgattcacga tcgaaggcca gcgtcggcga
      181 cattgagctg cgcgggcgct tgccaccgcc gacgcgattg gcaacgagtc cgtcggcgtc
      241 gcgcggcgcg aaggaaaaat cggtgagctg gttgttgagc aggaagccgc ggaccatcag
      301 ccgcgcgccg aaggcatgct cgatcgacga ggtcagcgcg accgcgtgac cggctgcgtc
      361 gacgaccgag aggtgcgtag tcgccggcac ttcgtggtcg tgcccgtcga tccagcccag
      421 cggcacggcc cctggattgc ccgctgtcgc gctccccatg ctgtgcgtgc cgatcagcgc
      481 ggcgcgctgg cgcaggtagc cgttgtcgtg caggccggcc ggcagcggta cgaaatcggg
      541 gtcggcgacg tagcggttgc gatcggcaaa ggccagccgc ccggcctcgc tgaagcggtg
      601 gatggcgtcg accgatggcg cgctgcccgg cgccgtgaac gccgtcatgt caccggcggc
      661 gagaatgccg aggatctgcg ctaccgcgag tccgcccgac gagggcggcg gcatgccgca
      721 gacgcgccag gcgaggaagc ccgtgcacag cggcgcgcgc tcgagcgccc ggtagccgga
      781 caggtcggcc gcggtcatcc gtcccggatt gctcgggtgg ccgttgaccg cggcgacgat
      841 cgcggccgcg agttcgcccg aatgcagcgc gtcggcgccc tcggtcgcca gttgctggag
      901 cgtcgccgcc agctcggggt tgcggagcac gttgccgact gccttggcgc ggccgtccga
      961 ttcgtagtag agcgcacggg cagccgggtc gcggcgcagg tagcggtcgt cggcgagcag
     1021 cttgtgcagg cgcgggctga ccgggaaacc cctttcggag agcgcgatcg cgtcgtcgaa
     1081 caaccgcgcc caggacaagc gcccgtcgcg gcgatgcgcc agttcgagca tgcgcaccag
     1141 gcccggtacg ccgaccgagc gaccgccgac gaccgcgtcg aagaacggca gcggcttgcc
     1201 gccggcgtcg aggaagagcg catcgtcggc agtggccggc gcggtttcgc ggccgtcgta
     1261 ggcgcgcacg cgctggccgt cgaagctgag caggaagccg ccgccgccga tgcccgagga
     1321 ttgcggttcg acaaccgcga gcaccagttg cgcggcgatt gccgcatcga cggcactgcc
     1381 gccggcatcg aggatgcgca ggccggcggc actggccgct ggatgcgcgg cgacgaccat
     1441 cgccttctcg gcgaataccg agggtttgcg ggtaaaaccg ctttcgcctt cgggcgccgg
     1501 ctgggcagtg gccgctgacg gcacgagcag cgccgccagc agcacgaaga ctccggcgcc
     1561 gcgccggacg cctggcgccg agcgctgcgg ctcagcccga gagcttcttg ccgtgctcgc
     1621 gcgaggcgtg gaacttgacc ttggggaagc gctcaccggt ggtctgcaga tagacccggt
     1681 tggcgacgag gtagacccag ttgtcatcga cgtccttggc cagcctggcg gcctggctgt
     1741 tctcgaattc gcgccactgc gcggggtcgt cgcaggttac ccagcgtgcg gcgacgatcg
     1801 gcgaggcctc gaacacggcc tcgacgctgt actcgctctt cagccgcgcg gctacgattt
     1861 caaactgcag cggacccacg gcaccgatca gcaggttgcc gccgccgtag ggctcgaaaa
     1921 cctggactgc gccttcttcg ccgagttcct tcaggccctt gtagagctgc ttggctttca
     1981 gcgggtcgcg cagcctgggc gagcggaaca gctccggcgc aaagtagggg atgccggtga
     2041 acgacaggcc gtcctcgtcg gccagcgtgt cgccgatctg caactggccg tggttgtgga
     2101 tgccgatgat gtcgccagca aaggcttcct cgctgcgcgc gcgcgtgttg gcgaggaagg
     2161 tcaccgcatt ctggatgcgg atctccttgc cgacacgctg gttcttcacc ttgatcccgg
     2221 gctcgtagcg ccccgagcag acgcgcagaa aggcgatgcg gtcgcggtgg ttggggtcca
     2281 tgttcgcctg gatcttgaag acgaaacccg agaacggcgt ctcgacggcg tcgacggcgc
     2341 gcttggtcgc atcccgcggc tgcggtggcg gcgcccagtc caccagcgcc tgcaggacct
     2401 cgcgcacgcc gaagttgttg atcgccgagc cgaagaacac cggcgactgg cggccggcga
     2461 ggaactcggc gtgattgaag tcgggacagg cggcaccgac cagttcgacg tcgtcgatga
     2521 agctgtcggg cgagacgccg agcttgtgcg ccaggtcttc gaggtgcgcg ccggtgactt
     2581 tttcgaaacc ctggtcgccg cgttcctggc ccggcgcgaa cttgagcacg acatggcttt
     2641 tcaggttcag cacgcccttg aatcccttgc ccatgccgat tggccaggtg atcggcgcgc
     2701 agctgatgtc gagcaccttt tcgatttcat cgagcaactc aatggattcg cgcacctcgc
     2761 ggtcgagctt gttgataaag gtgacgattg gcgtgtcgcg catgcggcac acctccatca
     2821 gcttgatcgt tcgctcctcg acgcccttgg cggcgtcgat caccatcaac gcgcaatcga
     2881 ccgcggtcag cacgcggtag gtgtcttccg agaaatcctc gtggcccggc gtgtcgagca
     2941 ggttgatgac gcagtcgtcg aactcgaact gcatcaccga cgaggcgacc gagatgccgc
     3001 gctgtttctc gacctccatc cagtcagagg tggcgtgccg atcagacttc ttcgccttga
     3061 ccgtgccagc catctgaatc gcaccgccga agaggaggag cttctcggtc agcgtggtct
     3121 ttccggcgtc ggggtgggag atgatcgcga aggtgcggcg gcgttttacc tcgggaccga
     3181 aggggctatc gatcatcgcg gggctcctcc gtggcggtgt gcgttgatcg cgtcgcgcgt
     3241 ggcgatcgct gcttcgcgcg ccttcgccgc gaaatcctcg cccttgccgg cgtagaggat
     3301 cgcccgcgag gaattgatca ttaatccgcc gccgccggct gtctgcccgg ccttgagcgt
     3361 ggcttcgatg tcgccgccct gggcgccgat cccgggcacc agcagcggca tctcgccggt
     3421 aatttctcgc acgcgggcaa tctcggtcgg gaaggtcgcg ccgacgacca gcgcgcagtt
     3481 gccactggcg ttccactcgc ttgccaccag ccgcgcgacg cgttcgtaga gcttttcgcc
     3541 gccgacgtcc aagaactgca ggtcactgcc gccggggttc gaggtccggc agagcaagat
     3601 cacgcccttg tccggatagg ccagataggg ctcgaccgag tcgcggccca tgtagggatt
     3661 gacggtgatg gcgtcggcgc gaaaacgctc gaaggcctcg atcgcgtact gctcggccgt
     3721 cgagccaatg tcgccgcgct tggcgtcgag gatgaccggc gtgccggggt gctgctcgtg
     3781 gatgtgtgca atcagcgcct cgagctggtc ttcggcgcgc tgcgcggcga agtaggcgat
     3841 ttgcggcttg aagcagcaga ccaggtcggc cgtggcgtcg acgatttcgc ggcagaaggc
     3901 gaggatcgcg tcgggccggc cagcaagatg ggccggggat ttcttcgggt cggggtcgag
     3961 gccgacgcaa aggagcgtat tgcggtcccg ccaggcgctg gcgagcgagg acatgaagtg
     4021 catggtggcg ggtttcgcga cgcgagagag gcgcgcagtt taccggatcg ccgtgtgact
     4081 gccgtcaccg gggagggcta tgccgccttg cgatcgccgt gaccgtcgcc atatgcccgg
     4141 gaagcccact gccaccggag tccacccatg tccgatccgc tgcatctcgt ctgcccgcac
     4201 tgccacgccg tcaaccgcat tcccgccgcc cggctcggtg accaacccgc ctgcggcaaa
     4261 tgccaccagc cggtgttttc cggccacccg acaacgcttg gcggcagcga cttcgacacc
     4321 cacgtcaatc gcagcgacgt gccggtgctt gtcgacttct gggccgaatg gtgcggtccc
     4381 tgcaagatga tggcgccgca gtttgcgcgc gccgcggccc agctcgaacc ccaggtccgg
     4441 ctggccaagg tcgataccga agccgagcaa gcgctggcgg cgcgctacgg catccgcagc
     4501 atcccgacga tggtgctgtt tcgcgacggc cgcgagatcg cgcggcagtc gggcgcgatg
     4561 agcgccgagg acatcacccg ctggacgagg caagcgctcg gcgcctgagc ccagccccag
     4621 ggacgcggcc taggaagcga gtccagacgc ttcggtctcg gtcgtttccc cggtgccggc
     4681 gccgagcgag ctttcgccgg ctgccaggcg cgacagcgca aaggcaaagc tggtgacgcc
     4741 ggcacccttg aacagcaggc agcgcttgcc ggtcttgcgg cactgctgct tgaggctgta
     4801 gtaggccgcg tgcccaacgc aatcggtcgg gcatagcacg gcgtcggcgc ttgcgatcat
     4861 ctcgggcagg cgggacaagg cttcctcctg gccgccatcg tggtgcagta ggcggatacc
     4921 gagccgctcg gcgagtgccc ggtagtggct gagcagcgaa gtccggccgc cgacgcagag
     4981 gatgcagcgg ctgcgcgaca ggccgttctg ttcggcgcaa cgcaggcagt cgtcgcgatc
     5041 gggcaccgtg ccgccggcgg ccgaggcggc aggtttctgc ggtttcagtg cagcggtcag
     5101 gatcccgtta agcgcctcgc gttcggctcg cagggcagtg gtttcgcgcc ggctcgcggc
     5161 cagttcgcgc tgcagacggt aggctcgttc ggcaagcccg tcgcagcgcg ccgcgaccgt
     5221 gcgcagcgcg gcgttctcgt cgctgagttg aagcaggcgg cgcgccatcc cggtcatcgc
     5281 gacgccggat tcgatttgat tcagtcttgc ctgcgccgcc tgaagcgcat cacggtcgcc
     5341 gcgcagggcg gcgttctcgg cgcgcaggct ggccagttcg gcactgccaa tctcggctcg
     5401 ctgagccagc gtccggcgcg tctcggccag ttcgcggcgc gcgtcgctgg catcccgttc
     5461 gaggaattcc agccgcctga cgtcggccgc cagtccggcg ccaatctgat gcgagagcat
     5521 gtggacctgc gcgtagagcg cctgccgcag agcctggtcg gcgtgcttgt ggctcagcgc
     5581 agcccaaagc ggtccggcga cctgcccgcg gtcgaagcac cggtcccagc gttcgcgtag
     5641 ttgctcggca ttgcgcagcg ctgcgaagtc gcgcaatgcc gcggcgtaca ggcggtcgag
     5701 caggcgctgc agggcctcgg ataccgcatt gcgcgcacca cagtagccga ccgcttcgac
     5761 gtggatgccg aagtcgtcgc aaggcttggc cgcgaaggca aagcggcgag caaccttcgc
     5821 cagttcatca accgggatac aggtaccgat ggtcaggcaa tggaatttgc ggtcgagtgt
     5881 ccacagcttg gtccgcgcgg cgcccttgga ggtggcagcc ggagcgagcg cagccagtcg
     5941 ggcaggatcg ccgggtatgg gcatcccgtc gcctgccggc atgcccgcca gtgcctggat
     6001 tccctgggtc aatgccgaag ttcgcgcggc catcatgcgc cgtctacccg agcgaggcgc
     6061 tcgctcatcc ggtcgcagag ttcgcgggtt tcgtcgtcga ggccgggcag gcaggtcagc
     6121 cgttcaagca ggtcggcggc cttccaggcg gcgtgacggc agcccgagag ctggtggcgc
     6181 agcagttcgg agagtgccgc ggcgaagagg ctggcgcgcg cggcgaacgg cgtgggcgtg
     6241 gccggctggc gcagggtttg tggcgaagcg gctggcgcaa gggcaagcgt cgaggcaggt
     6301 gcgagggcgt gcatggcaag tctccattca tctcattgcg acgatggctg gcttgccgaa
     6361 cccgacgggc ggctggctgg ctagcggcgg accgggtgat ccgccgcggg gtttgaatca
     6421 actgctgcgg gctggcttca ggacgcctcg cctatctgcg tctgcaggta gttgtggatg
     6481 ccgaccttgt cgatcaactc gagctgagtc tcgagatagt cgatgtgctc ctcggtgtcg
     6541 tcgaggattt cctgcaggat ggcgcgcgaa acatagtcgt tgatgctctc gcagtaggtg
     6601 atcgccgcct tggtgtcggc gtgcgcgccg gtttcgagct tgaggtcgcc ggcgatgcac
     6661 tcgggaacgt tctcgccgat caggagcttg ttcagtgcct gcaggttggg caggccttcg
     6721 aggaacagca cgcgctcgat cagcttgtcg gcgtgatgca tctcctcgat cgactcctcg
     6781 tactcgtgct tgccgagggc attgaatccc cagttcttgt acatccgggc gtgcaggaaa
     6841 tactggttga ttgcggtcag ctcattgacc aggcacttgt taagcatctg gatgactttc
     6901 ttgtcgccct tcatgcgttt ctccctttag gctgcggcaa gttgacaaac cggtcgttgg
     6961 cgcttggttg cgggcgtcgc ggtctggtgg agtcgtctgc gggcgcaatc cgagcagcga
     7021 ccgcagcacg ttccgacgcc gagcgactcg cggagttccc tcagcgtggt gcagccggcg
     7081 gcgacggcgt tgtcgatgtc gctttcggtg accgcgttgc aaacgcagac gtacatgagg
     7141 gttctcctta cttggtcagg atcagcttgc cgaggcgggt ttcgcgcaaa cgataggttt
     7201 cgtcgcggtg gcggatggtg agttcgctgc gttgcccgag cagcgactgg ctgtcgacgc
     7261 tcaaggtctc gggtccggcc cagggcaagg aatcgcggcc gcgctgcgac gatggccatg
     7321 ctgacgttgc tttcgaggtg gggtgtgcca tcgcttcacc ggccactttg cagatgagaa
     7381 gcattctcat atagagcgtt gccgatgtca aatactttct ggggatttcg agccgcgacc
     7441 tcgggccgct taactgaatt cggcgagcgg caggtcttcg ggatggtcaa tgtcccgcaa
     7501 ggttctcccg aaatgcaggc gcagcccggc ggctgcggcg cgggcccggg tctggtccat
     7561 gacgcggggg ctgccccaat ctacgccggc gaacacagca gcactcgggc gggtcagccc
     7621 gatcagcacg tagccgccgt ccgccgccgg gaccagcgcg acgtcgcgcg ggccagcgag
     7681 ctgttgcgcg gcattgcgca ggtcgtcgac gctgagctcg gggcagtcgg tgccgatcag
     7741 cagcgcggcg tggtggtcgg tcagtgcctc cgccagtgcc gtttgcatgc gttcgccgag
     7801 gtcggcgccg ttctggacgt gcatgctggc accgtggcga gtggcggcga cgacgagcgc
     7861 cgggtgcctg gcgtcgggcg tacaccaaag ctgcagcgag gcgaagcggc cggcggcggc
     7921 ggttgtcagc gtccgttcca acaggcgcac ggccagtgcc gctgcagcgt cggcaccgat
     7981 tgccgggatt aggcgagtct tgactgcgcc cggaacaggc gccttggcaa agacgagcag
     8041 cgcgacgcgt cgaccgctgc tacgtcggac gcgccgcgcc agcgggcccg gcgacgcggt
     8101 gtcgtcggtc gcgtagccgt atcgccgggc cagcgcagct ggatcggcgc cacgccagta
     8161 ggccaggcgc aggcgccaca tcagaccaat cgtgcgccag aaaccgtgct cgtcccagcg
     8221 ccggcccgag gtctgcgccg gcccgggcag gcaggccgga cggctatggc ggcgcagctt
     8281 cgttgacagc gcgatgtcct ccattagcgc ctggtctgga aatccgccgg cggcgacgaa
     8341 ggttgcgcgg tcgacgaaga tcgcctggtc gccggtggcg atgccggtta aacgcgagcg
     8401 ttggttcatc agccaggcga ccagcggcag cagcgggtgg cggccaatga tctgcacgtc
     8461 gaatcggccc cagcgggccc cccggcgcag cgcgccggcg atatgttggt cggcgtctgc
     8521 gggcagcgtg gtgtcggcgt ggagaaaaag cagcgtcgcg ccattggcgc gctcggtgcc
     8581 ggcgttcatt tgccgggcgc gaccgcgccg gctgccgacg acgtagtcgg ccagcggcgc
     8641 ggcgagcgcc gccgtgtcgt cgtcgctgtc gccgtcgacg acgatcagtt cggcgccgcg
     8701 tgcgcggagc ggttgcagtc cctcaagtgt ggcctcgatg cgcgcggcct cgttcagcac
     8761 cgggacgacg atggtcagca cgcaggtcaa gagatctccg gtggggcagt ccgcgggcga
     8821 gcctagcggg cgtcgttgag gagccagtcg tagtcgagat ggcgaatggt gacggtatcg
     8881 gcggtgatcg cagcgagcag tgtttcccgg tcggccgcct gcggcgcgag ttccgccgcg
     8941 tgcgccgcga agagctgcag caggcccttg cggcgatcgc cctgccgcgc aaaatcgtcg
     9001 ccgtaccagt cgaagatctt cgagacctga agcgtgttgc tgcgtgcgtc gtagcggttt
     9061 cggctctggt cagcgaggaa gcgacgcatc gcatcggcga ggtccgcatc gagggtgtcg
     9121 gccgtgaatg cggtgttgcg cagcatcggg cagccgatcg aggcgcagac cactgcgacg
     9181 tggattcgcg gctcgtcgta ggcgccgggc gcgcggatta ttccgtgttc gatctcgtcg
     9241 aggtggcgtt cctcgccgag cagcttgaag aagcgctgct tccacggcga gccgaaccag
     9301 ccgccgatgt ccttgatcga gtccagcttc ggatagcgcg tgaggatcag ttcgaccgta
     9361 taggcgttgt aggcgttgat caggaaggcg aggcgctggg gcttgctcca gcccgcgaac
     9421 tcgtctgccg tgaccgacga cagttggtcg aggtaggccc gcagattggc gcgatccttg
     9481 gccaccgcgg cgtagcgtac ggccgaggcg ttgccgtcgg gcgcgacgac cacgtattgc
     9541 ttcagcagcg cgttccacgc cgcgtggttg ttgtcgaagg ccaccgcggc gctgctggct
     9601 agcagcagca gggtgacgat caggacgcgc ttcatcgcgc cgccccagca gcggcgttcg
     9661 cgggcgagcc gcgacgccag ccgtggaatc gctcgaccca gcgcagcaga ccctgcggcg
     9721 cgtgcgcctt cttccagttg ccggccgcgt acttgttggc ctcggccagc gtcgggtaga
     9781 tgtggatcgt gccgagcagc ttattgagcc cgataccgtg tcgcatcgcc aggacgaact
     9841 cggcgatcag gtcaccggcg tgctcgccga ccagcgtaac gccgaggatg cggtccttgc
     9901 ccggaaccgt gagcaccttg acgaagccgt gggcctcccc gtcggcaatc gcccggtcga
     9961 ggtcgtcgat gccgtagtgc gtgacttcgt aggcgatgtt gcgttctctg gcctcgagtt
    10021 cgttgatgcc gacgcgcgcg acctcaggct cgacgaaggt cgcccaggga atcaccgagt
    10081 aatcggcgcg gaacttcttc agtgggtcga acagtgcgtt gaccgcggcg taccaggctt
    10141 gatgcgcggc cgtgtgcgtg aactggtagg gaccggcgac atcgccggcg gcgtaaatgt
    10201 tgggaaattt cgtctgcaag tactcgttga cctcgaccgt acggccaacc ggaatgccga
    10261 gttcctccag cccgtagccc ttgagattgg cggcacggcc gacggcgacc agcaccgcgt
    10321 cgaaggggat gcggacttcg gcgcctgcgt gctcggcgac gagaatcttc tctccgttgt
    10381 cgacgaggaa gcgcttggcc tgatgactca ggcgcaggtc gattccctcg gcgcgaaagc
    10441 gcgcggcgac gagttcggag acctcggagt cttcgcgcgc catgatccgc tcgcccatct
    10501 cgacctgcgt cacctctgcg ccgaagcggg cgaaggcctg cgtcagctcc gagccgatcg
    10561 ggccgccgcc gagcacgacc agccgcttcg gcagttcgcg cagcgaccac acggtgtcgg
    10621 aggttaggta gccgacttcg tcgaggccgg ggataggagg cacgaagggg cgcgcgccgg
    10681 cggcgatgac gatgctgcgc gtgctcaggg ttttggcctt cccatcactg cgtgtgattt
    10741 caaccgacca aggcgagacg atcttcgccg tgccttcgac gacctcgacc ccgagtccgg
    10801 tgtagcgctc gaccgaatcg tgcggttcga ccgcctgtac gacggcctga acgcgttcca
    10861 tgacctcggc gaaatcgaag gcagcgctcg attcgcggat gccgaattcg cgcgagcggg
    10921 agacgtgcga gaggaacttc gccgatcgga tcagagcctt cgacggcacg cagccggtat
    10981 tgaggcagtc gccgcccatt ttgtgcttct cgaccagcgt caccttggcc ttcaccgcgg
    11041 cggcgatgta ggccgtcacc agaccggccg acccggcgcc gatcaccacc aggttgcggt
    11101 cgaaatgcac cggccgctgc cagccggcgt agatcttgcg cgctttgatc cactcgacaa
    11161 tttttttggc gatcagcgga aagataccga gcagcgcgaa cgcaccgagc agcccgggcg
    11221 acagcaagcc ggccgccgag tcgatttgtg cgagctgcgt gccggcgttc acgtagacga
    11281 tggtgccggc gaacataccg agctggctga cccaggcgaa ggtgatcgcg cgcatcggcg
    11341 tcaggcccat cgccaggttg atcgcgaaga agggaaacac cggcaccagg cgcagcgtaa
    11401 agagatagaa ggggccgtcc ttggcgatgc cggcgtcgat tgccttgagc ttgtcgccga
    11461 atttcgcgcg caccgtgtcg cgcaggacga agcgcgcggc gagaaaggcc agcgtcgcac
    11521 cgagcgtcga ggcaaaggag acgatcaccg tgccccagag caggccgaag atcgcccctc
    11581 cggccagcgt cagcaccgcg gcgccgggca gcgacaggcc ggtggcggcg acgtaggcga
    11641 cgaaatagat cgccgcggtg gacagcgggt tggcgtcgcg atacatggtg atcgcctcct
    11701 gctggctctt caggtagtcg
//
LOCUS       DATDAA010000252         7851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_507732,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000252 DATDAA010000000
VERSION     DATDAA010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507732"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2219)
                     /locus_tag="VIS73_07965"
     CDS             complement(<1..2219)
                     /locus_tag="VIS73_07965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY5763129.1"
                     /translation="MTRRSAHDPPRHELRWPQYVRERIAERIAITSALITLAFALSIG
                     AISLAYTLHLTREAAQMRLEEDTRDAANRLSQLVDGAFNDIRLLAGNPTVIETLAGRP
                     LSPSALAPLLVSFHPAGREPVILCAANDAGEILACDRRDLPVDPVVLDATPSSAQLLA
                     RGELTQRGEQPHLRLTAPALDANRRLRGFVVGEYAIERLIISALGNPANYYHLHLVGN
                     SPLADEGDLFMRGAMEQAKVHDRPLDVDPALRPLALRLHLGIDDGMIWTPTRNVLLVY
                     VAVLAALLPLVYLAARKSGYALSARLRRLAAETRALASGEQDELPTGHYGSDEIGDLA
                     RAFGEVIGDLRQSRAELATRVDQRTRELQASLDEVRALNTAIGQSAVSVIITDCNGTI
                     RFVNADFERVTGYRRQEIVGQTARVLKSGRTLPFEYGSLWATITAGKIWRGEFENRRK
                     DGSHYFAQASIAPIRSDAGEITGFVAVEEDITERKQFEREIRDRENYLRTLVDSLPDT
                     LLVLDHAGRIQQLRAPDAHLGSAPGTWTSDWCLGRHYAEVLPREIAGPITTILPEILQ
                     GPEHREFAYTIRHAGEKRHYAASIRRIDDEDPARAGCVILSRDVSEAFALQAKLIRAE
                     ARFRTLLEDIDTVAVQGYDRRRQVIFWNRASERLYGWRSDEAIGRRLEDLIVADDVRD
                     STIAAVRAAIEKGQAVPPGECTVRRKNGIPVYVYSCSAVQTNLAGEPELFRVDIDLS"
     gene            complement(2679..3737)
                     /locus_tag="VIS73_07970"
     CDS             complement(2679..3737)
                     /locus_tag="VIS73_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEY5763130.1"
                     /translation="MKFPPDGQRHLAASVAPAFVTGMIAALQARGADLAPAFKAAGLG
                     TPWPNARSRVERVPVSYYAALYNALAEAFDDEAFGMFAQPLRCGSFECLCRVVADGNN
                     LGVALARAAQYLRIILPEMTVSLTSRRESAELRLTACGAFAQRAATDATRIFAFEWLL
                     RLLHALACWLLARAIPLDSVEFPFPRPEHADDYALIYTEHSRFRQESNTLVARFPLDL
                     LRMPLRRDARALERFLDGAPGKIALLYRRDRELANHIRELVARSLPLVPSVNTIADSL
                     ALSPRTLHRRLSEEGSNCRAIIDAVRRDRALARLATSNQPLSTIAAELGFIEASAFYR
                     AVVSWTGASPSAYRNRLK"
     gene            3901..7161
                     /gene="icmF"
                     /locus_tag="VIS73_07975"
     CDS             3901..7161
                     /gene="icmF"
                     /locus_tag="VIS73_07975"
                     /EC_number="3.6.5.-"
                     /EC_number="5.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0047727 - isobutyryl-CoA mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused isobutyryl-CoA mutase/GTPase IcmF"
                     /protein_id="HEY5763131.1"
                     /translation="MTDLSIAGKLAPHKPRHKIRFITAASLFDGHDASINIMRRILQG
                     TGCEVIHLGHNRSVQEIVNAALQEDVQGIAITSYQGGHVEYFKYMMDLLKAGGGENIK
                     VFGGGGGVIVPAEIDELHAYGVTRLYSPEDGQRMGLQGMINDVVQQADYDLSPLAPQT
                     DAEALKALRSGNRRALAQVVTVLENGAAPYKGAVIQAAAEIKTPVLGITGTGGAGKSS
                     LTDELIRRFRLDQEDALTIAIVSIDPSRKRTGGALLGDRIRMNAIEHPNVFMRSLATR
                     DTGSEISAALPEVIAACKLSGFDLIVVETSGIGQGDAAIVEHVDASLYVMTPEFGAAS
                     QLEKIDMLDFADFVAINKFDRKGAEDALRDVRKQVQRNREAFGTPTEEMPVFGTMAAR
                     FNDDGVTALYQALLPVLVGKGLKVKRGKLPVVKTRQSSRGRAIVPAERVRYLADIADT
                     VRAYHAEVKKQVALARERQALRLSKGIFEACGKDAKDFDELVVAKDGELSAAAKKLLE
                     LWPKTRELYAQDEYVVKIRDKEIRTGLIKTSLSGSKIRKVSLPQYEDDGETLRFLMKE
                     NLPGSFPFTAGVFAFKRENEDPTRMFAGEGDAFRTNRRFKKVSEGMPAHRLSTAFDSV
                     TLYGYDPDSRPDIYGKIGNSGVSIATLADMKVLYDGFDLCCPTTSVSMTINGPAPIIL
                     AFYFNTALDQQIDKFRSDNGREPTEDEVQKIRAWTLSTVRGTVQADILKEDQGQNTCI
                     FSTEFALKMMGDIQEFFVHHDIRNFYSVSISGYHIAEAGANPISQLAFTLANGFTYVE
                     AYLARGMHIDDFAPNLSFFFSNGMDPEYTVIGRVARRIWAVAMKKRYGANERSQKLKY
                     HVQTSGRSLHAQEMDFNDIRTTLQALIAVYDNCNSLHTNAYDEAITTPTDESVRRAMA
                     IQLIINREWGLAMNENPNQGSFIVDELTDLVEEAVLKEFEALASRGGVLGAMETGYQR
                     GKIQEESMLYEHRKHDGSYPIIGVNTFLNPKGSAQPEIELARSSEDEKQSQITRLNDF
                     QTRNAAESAKWLARLKQTVIDNGNVFEVLVDAVRYCSLGQITSALYEVGGQYRRNM"
     gene            7250..7585
                     /locus_tag="VIS73_07980"
     CDS             7250..7585
                     /locus_tag="VIS73_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY5763132.1"
                     /translation="MDWIFEATEDEFERVVVAASHERPILVDISADWCAPCRVLSPVL
                     EQVIAEFDGAVHLAMVDADENMRIAGRHKVRGFPTVIAYRNGVEVDRFHSAQTLGYVR
                     DFIERQLVG"
BASE COUNT         1430 a         2444 c         2626 g         1351 t
ORIGIN      
        1 tcgctgaggt caatgtcgac gcggaacagc tcgggttcgc cggccaggtt ggtctgtacc
       61 gccgagcacg aataaacgta gaccgggatg ccgttcttgc gtcggacggt gcattcgccg
      121 ggcggcacgg cctgcccttt ctcgattgca gcgcgaaccg cggcaatcgt gctatcgcgg
      181 acgtcgtcgg cgacaatcag gtcttccagg cggcggccga tggcttcgtc gctgcgccag
      241 ccgtagaggc gctcgctggc gcggttccag aagatcacct ggcgccgccg gtcgtagccc
      301 tgtacggcga cggtgtcgat gtcctcgagc agcgtgcgga agcgcgcttc ggcacggatc
      361 agcttggctt gcagcgcgaa ggcctcgctg acgtcgcgcg agaggatgac gcagccggcg
      421 cgtgccgggt cctcgtcatc gatgcgccgg atcgatgccg cgtaatgacg cttttcgcca
      481 gcgtgacgga tcgtgtaggc gaactcccgg tgttcgggac cctgaaggat ttccggcagt
      541 atcgtcgtga tcggcccggc aatctcgcgc ggcaggactt cggcgtaatg gcgcccgagg
      601 caccagtcgc tggtccaggt gcccggtgcc gaaccgaggt gggcatcggg cgcgcgcagc
      661 tgctggattc gcccggcatg atcgagaacc aacagcgtgt cgggcagcga atcgaccagg
      721 gtgcgcaggt agttttcgcg gtcgcggatc tcgcgctcga actgcttgcg ttcggtgatg
      781 tcttcctcga ccgcgacgaa gccggtgatc tcgccggcat cgctgcggat cggtgcgatc
      841 gaggcctgcg cgaaataatg cgagccgtcc ttgcgccggt tctcgaattc gccgcgccag
      901 atcttgccgg cggtgatcgt cgcccacagc gagccgtact cgaaaggcag cgtccgcccc
      961 gacttgagaa cccgggcggt ctggccgacg atttcctgac ggcggtagcc ggtcacgcgc
     1021 tcgaagtcgg cattgacgaa acggatcgta ccgttgcagt cggtgatgat caccgagacc
     1081 gcgctctgcc cgattgcggt attgagcgcg cggacctcgt cgagcgatgc ctgcagctcc
     1141 cgggtacgct gatcgacccg cgtcgcgagt tcggcgcgcg actggcgcag gtcgccgatg
     1201 acctcgccga aggcgcgggc gaggtcgccg atttcgtcgc tgccgtagtg accggtcggc
     1261 agttcatcct gttcgccgct ggcgagggcg cgcgtctcgg cagccagccg gcgcagccgc
     1321 gccgacagcg cgtaacccga cttgcgggcg gccagataga cgagcggcag cagcgcggcg
     1381 agcacggcga cgtacaccag caaaacgttg cgggtcggcg tccagatcat cccgtcgtcg
     1441 atgcccaggt gcaggcgcaa tgccagtggg cgcagcgcgg gatcgacatc gagcggccga
     1501 tcgtggacct ttgcttgctc catggcgccg cgcatgaaga ggtcgccttc gtcggcaagc
     1561 gggctatttc ctaccaggtg caggtggtag tagttggctg gattgccaag cgccgaaatg
     1621 atcagtcgct cgatcgcgta ttcgccgacg acgaaaccgc gcagccggcg gttggcgtcg
     1681 agcgccggcg cggtcaggcg cagatgtggc tgctcgcccc gctgcgtcag ttcgccccgg
     1741 gccagaagct gtgccgacga gggtgtggca tcgagaacga ccggatcgac ggggaggtcg
     1801 cggcggtcac aggcgaggat ttcaccggcg tcattggcag cgcagaggat gacgggttcg
     1861 cgtccggccg gatggaaact caccagcaat ggagccagcg cgctcggact gagcggtcgg
     1921 ccggccagag tttcgatgac cgtcgggttg ccggcaagca ggcgaatgtc gttgaacgcg
     1981 ccgtcgacga gttgcgacag ccggttggcg gcgtcgcggg tgtcttcctc aagacgcatc
     2041 tgggccgctt cacgcgtgag atgcagcgtg taggcgaggc tgatagcgcc aatcgagagt
     2101 gcgaaggcga gcgtgatcag ggcgctggtg atcgcgatgc gctcggcaat ccgttcgcgt
     2161 acgtactgcg gccagcgcag ttcgtggcgc ggcggatcgt gtgcgcttcg ccgcgtcatc
     2221 gccgtcgctc cacccgggtg cccctgatgt cccgcagtgc gttacgcgat gcgggccagc
     2281 gggaaatggt ggggctccgg cgtttcggac cgccgcgaca ggcaaggcag cgtgcgaaac
     2341 atagcgcgcc cgcagcccag gccagcggct gaacggcggc tggacgctcc tgcggcgcgt
     2401 ttgccgaatc cggatgcctg acgctcatct tggcgcgctc cctgtcgccc ggtcggccgc
     2461 tctagttgcg gctcgatttt ttgcttacac cgatcttacg cctcttggtc cgatcggcga
     2521 ggcgtcccgg cgtcagctgc cgtgacaggt tccacaagtc cgggtggagt tttctcggcg
     2581 ttcgcgggct gcaagaatgg gcgaatcgaa gggggtgcca tgcgacgcgc gctaccgagg
     2641 gcatgcagtg gtttgtcggg cccgggtttg gcggcggcct acttcaggcg atttcggtag
     2701 gcgctcggcg aggcgccggt ccagctgacg acggcgcgat agaaggccga tgcttcgata
     2761 aagccgagct cggcagcgat tgtcgacagc ggctggttgc tggtggcgag gcgggccagc
     2821 gcccggtcgc ggcgtacggc atcgatgatc gcccggcaat tgctgccctc ctcgctgagg
     2881 cggcggtgca gcgtccgcgg cgacagcgcg agcgaatcgg caatcgtgtt gacggacggc
     2941 accaagggaa gcgaacgggc gaccagttcg cggatatggt tcgccaattc ccggtcccgc
     3001 cgatagagca gcgcgatctt tcccggcgcg ccgtcgagaa agcgctccag cgcgcgggca
     3061 tcgcgccgca gcggcatgcg cagcaggtcg agcggaaagc gcgcaacgag cgtattgctc
     3121 tcctggcgga agcgcgagtg ctcggtatag atcagcgcgt agtcgtcggc atgctctggt
     3181 cgcgggaacg ggaactcgac cgagtcgagc gggatggcgc gggcgagcag ccagcaggcc
     3241 agtgcgtgca gtagccgcag cagccactcg aaggcaaaga tgcgggttgc gtcggtcgcc
     3301 gcacgttggg caaacgcgcc gcaggcggtc aaccgaagct cggccgactc gcgtctggat
     3361 gtcagcgaca ccgtcatctc gggcaggatg attcgcaagt attgcgcggc acgcgccagt
     3421 gcaacgccga gattattgcc gtcggccacc acgcggcaca ggcactcgaa actcccgcag
     3481 cgcaggggct gcgcgaacat gccgaaagct tcgtcgtcga aggcttcggc gagcgcgtta
     3541 tagagagccg catagtagct gaccggcacc cgctccacgc gcgaacgtgc gtttggccag
     3601 ggcgtgccga ggccggcggc cttgaaggct ggcgcgaggt cggcgccgcg tgcctgcaat
     3661 gccgcgatca ttcctgtgac gaaggccggc gcgacgctcg cggcgaggtg ccgctggccg
     3721 tctggcggaa atttcacctt ttctcgaaac ggcataacaa gagcatgaat ttaaaaggaa
     3781 acgagaactg gcggattttg cacgatctgc ggcggctgcg gcaattcctt cactggcgct
     3841 ggcagtctag gattgaggta tcgactgatg cgcgctgcct agcgcgatcg aggagccaac
     3901 atgactgacc tgtccattgc cgggaaactg gcaccgcaca agccccgcca caagattcgc
     3961 ttcattaccg cagcgagcct gttcgacggc cacgacgctt cgatcaacat catgcggcgg
     4021 attctccagg gcaccggttg cgaggtcatc caccttggtc acaaccgttc ggtgcaggag
     4081 atcgtcaacg ccgcattgca ggaagacgtg cagggcatcg cgatcacctc gtaccagggc
     4141 ggccacgtcg agtacttcaa atacatgatg gatctgctga aagccggtgg cggcgagaac
     4201 atcaaggttt tcggtggtgg tggtggcgtc atcgtgccgg ccgagatcga cgagcttcac
     4261 gcctacggcg tgactcgcct gtattcgccg gaagacgggc agcgcatggg cctgcagggc
     4321 atgatcaacg acgttgtgca gcaggccgac tacgaccttt cgccgctggc gccgcagacc
     4381 gatgccgagg cgctcaaggc tttgcgctcc ggcaatcgcc gcgcgctggc gcaggtcgtc
     4441 acggtgctcg agaacggcgc tgcgccctac aagggcgccg taatccaggc ggctgccgag
     4501 atcaagacac cggttctcgg catcactgga accggcggcg ccggcaagtc ctcgctgacc
     4561 gatgagctga tccgccgctt ccgcctcgac caggaagatg cgctgacgat cgccatcgtt
     4621 tcgatcgacc cctcgcgcaa gcgcaccggc ggtgccttgc tcggcgaccg tatccgcatg
     4681 aacgcgatcg aacaccccaa cgtcttcatg cgttcactgg caacgcgtga taccggaagc
     4741 gagatatcgg ccgcgctgcc cgaagtcatc gctgcctgca agctctcggg atttgacctg
     4801 atcgtcgtcg agacctcggg catcggccag ggtgatgcgg cgatcgtcga gcacgtcgat
     4861 gcctcgctct atgtgatgac ccccgagttc ggtgccgcca gccagctcga aaagatcgac
     4921 atgctcgact tcgcggattt cgtcgcgatc aacaagttcg atcgcaaggg cgccgaggat
     4981 gcattgcgcg acgtccgcaa gcaggtccag cgcaatcgtg aggctttcgg cacgccgacc
     5041 gaggagatgc cggtgttcgg cacgatggcc gcgcgcttca acgacgatgg cgtcacggcg
     5101 ctctaccagg ccttgctgcc ggttcttgtc ggcaaagggc tgaaggtcaa gcgcggcaag
     5161 ctgccggtgg tgaagacacg tcagtcgtca cgcggccgcg cgatcgtgcc ggccgagcgg
     5221 gtgcgctatc tggcggacat cgccgacacc gtgcgtgcct accacgccga ggtgaaaaag
     5281 caggtagcgc tggcgcgcga gcgacaggcc ctgcggctct cgaagggcat cttcgaggct
     5341 tgcggcaagg acgcgaaaga cttcgacgag ctggtggtcg cgaaggacgg cgaactctcg
     5401 gcggcggcca agaagctgct cgaactgtgg ccaaagaccc gtgagctata tgcgcaggac
     5461 gaatacgtcg tgaagatccg cgacaaggag atccgcaccg gcctgatcaa gacctcgcta
     5521 tcgggcagca agatccgtaa ggtgtcgctg ccgcagtacg aggacgacgg cgaaacgctg
     5581 cgcttcctga tgaaggagaa cctccccggg tcgttcccgt tcaccgccgg cgtgtttgcc
     5641 ttcaagcgcg agaacgaaga tccgacccgc atgtttgccg gcgagggcga cgcatttcgc
     5701 accaaccgcc gattcaagaa ggtgtcggag ggtatgccgg cgcatcggct gtccactgcc
     5761 ttcgattcgg tgacgctcta cggctacgac cccgattcgc gccccgacat ctacggcaag
     5821 atcggcaatt cgggcgtctc gatcgcgacg ctggccgaca tgaaggttct gtacgacggc
     5881 ttcgatcttt gctgcccgac gacctcggtg tcgatgacga tcaatgggcc ggcgccgatt
     5941 atcctcgcct tctacttcaa cacggcgctc gaccagcaga tcgacaagtt ccggtccgac
     6001 aacggccgtg agcctaccga ggacgaagtg cagaagatcc gcgcgtggac gctgtcgacc
     6061 gtgcgcggca ccgttcaggc cgacattctc aaggaggacc agggccagaa cacctgcatc
     6121 ttcagcaccg aattcgcgct gaagatgatg ggcgacatcc aggagttctt cgtccaccac
     6181 gacatccgca atttctactc cgtgtcgatc tcgggctatc acatcgccga ggccggcgcc
     6241 aacccgatca gccagcttgc cttcacgctc gccaacggct tcacctacgt cgaggcctac
     6301 ctcgcgcgcg gcatgcatat tgatgacttc gcgccaaacc tgtccttctt cttcagcaac
     6361 ggcatggacc ccgaatacac ggtgatcggc cgcgtcgcac gccgcatctg ggccgtggcg
     6421 atgaagaagc gctacggtgc aaacgagcgc tcgcagaagc tcaagtatca cgtgcagacc
     6481 agcggtcgtt cgctgcacgc gcaggagatg gacttcaatg acatccgtac cacgctgcag
     6541 gcgttgatcg ccgtttacga caactgcaac agcctgcaca ccaatgccta cgacgaggcg
     6601 atcaccacgc caaccgacga gtcggtgcgc cgggcgatgg cgatccagct gatcatcaac
     6661 cgcgaatggg gcttggcgat gaacgagaac cccaaccaag gcagcttcat cgtcgacgag
     6721 ttgaccgacc tcgtcgaaga ggctgtgctc aaggaattcg aagcgctagc ctcgcgcggc
     6781 ggtgtgcttg gcgcgatgga aaccggctac cagcggggca agatccagga agaatcgatg
     6841 ctctacgagc accgcaaaca cgacggttcg tacccgatca tcggcgtgaa caccttcctc
     6901 aatcctaagg gaagcgcgca gccggaaatc gaactggcgc gctcaagcga agacgagaag
     6961 cagagccaga tcacgcgcct gaacgatttc cagacacgca acgctgccga atcggcgaag
     7021 tggctcgcca ggctcaagca aacggtgatc gacaacggta acgtgttcga agtgctcgtc
     7081 gatgcggtgc gctactgctc gctcggacag atcaccagcg cgctgtacga ggtcggtggg
     7141 cagtatcggc gcaatatgta ggcacgggcg cgggctaccc cggcgggtga ttggtcgcgc
     7201 gccggcacgt cgctagactg gcgctcccga caacgccggc ggtttccaaa tggactggat
     7261 attcgaagct accgaggacg agttcgagcg ggtcgtcgtg gccgcttcgc acgagcggcc
     7321 gatcctggtc gacatcagcg ccgactggtg cgcaccctgc agggtgctta gcccggtgct
     7381 agaacaggta atcgccgaat tcgacggcgc cgtccatttg gcgatggtcg atgccgacga
     7441 gaacatgcgc atcgccggac ggcacaaggt gcggggattc ccgaccgtga tcgcctaccg
     7501 caacggtgtc gaagtcgatc gcttccacag cgcgcagacg ctgggttacg tccgcgattt
     7561 catcgaacgg cagcttgtgg gctagccgcc ctgcaacggc gccaccatct ggcgggtggc
     7621 tgcttcccgt cggcgtgttt gtaagggcag cctaactcgg ccaggggttg tcagccgccc
     7681 gttttctgca ttgctgtgac gcccgagaaa ctcgttcccg ggttggaatc gctgaattcg
     7741 aatgcgacgc ctgcatggct cgcacccgca ccgaagaatg ccccagagat gctcgcggcg
     7801 ctgcaggtcg tgctggtgac gggcgcagtg gatcctgaag cgacggtgcc a
//
LOCUS       DATDAA010000253         2243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_158167,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000253 DATDAA010000000
VERSION     DATDAA010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158167"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VIS73_07985"
     CDS             complement(<1..696)
                     /locus_tag="VIS73_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY5763133.1"
                     /translation="MKTLICGSIAFDNIMVFKDHFRNHILPDQIHILNVAFLVPEMRR
                     EFGGCAGNIAYNLKLLGGEPLIMATVGDDAPPYLERLELFDLPRDHVRHVPGTFTAQA
                     FITTDLADNQITAFHPGAMSFSHLNKVEAASNVGLGIVAPDGREGMLDHARGFADAGI
                     PFVFDPGQGMPMFSGDELLEFLRLAEFACFNDYEARLLCDRTGRSLEMLAQEVTALIV
                     TRGGEGSNIYAGGQ"
     gene            complement(722..1729)
                     /locus_tag="VIS73_07990"
     CDS             complement(722..1729)
                     /locus_tag="VIS73_07990"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HEY5763134.1"
                     /translation="MAVIARCPHCDTSFRVTPKQMKAKSGKVRCGSCKAVFNVLEHLI
                     EDSPDSIAPKLDLQWPEKINRALTNVAAEREAEAAVPPAAEVIGDNQDAGVAVPAVPA
                     AEAPADPEPATPPIASEAAPEAGAEAAESREDLAQTVREIRTAPGYNRWAESPLSSGS
                     ISIAAPAGEMSPVARTLWPVVSLVALVALLLQGAYHWRTDLAAHLPAARPFLEALCLQ
                     FHCTIDLPRETTFVAIESSTLQTDPARPGLVLLDATLRNSASYAQAYPNLELTLTDSA
                     DRPLVRRVLFPGDYLADSGDAGRARIARGFPPLGESNLRLWIDVSEIGAVGYRLYLFY
                     P"
     gene            complement(1729..>2243)
                     /locus_tag="VIS73_07995"
     CDS             complement(1729..>2243)
                     /locus_tag="VIS73_07995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY5763135.1"
                     /translation="ELDPGLAFGTGSHPTTRLCLEWLVEHLDRGVDVLDYGCGSGILA
                     IAAAKLGAGPVVGTDIDPQAVTAARDNAARNGVAIRFESVSAGHDGRYHIVVANILAN
                     PLKALAPALCAHVDDGGRLALSGILVEQADELIANYAPWIALEVAGSREGWVCLAGQK
                     PMLMPMPGAS"
BASE COUNT          373 a          700 c          802 g          368 t
ORIGIN      
        1 ctggccgccg gcgtagatgt tcgaaccttc accaccgcgt gtgacgatta gcgcggtgac
       61 ttcctgcgcc agcatctcga gcgagcgccc ggtgcgatcg cacagcagcc gtgcctcgta
      121 gtcgttgaag caggcgaact cggcgaggcg caagaactcg agcagttcgt cgcccgagaa
      181 catcggcatg ccctggcccg ggtcgaaaac gaagggaatc cccgcgtcgg caaaaccacg
      241 cgcgtggtcg agcatgcctt cgcggccgtc gggcgcgacg atgccgaggc cgacattgct
      301 ggccgcctcg accttgttca gatgcgaaaa gctcatcgcg ccggggtgga aggcagtgat
      361 ctggttgtcg gccaggtcgg tggtgatgaa ggcctgggcg gtgaaggtgc ctggcacgtg
      421 ccgcacgtgg tcgcgcggca ggtcaaacag ttcaagccgt tcgaggtagg gcggcgcgtc
      481 gtcgccgacg gtcgccatga tcagcggctc gccaccgagc agtttgaggt tgtaggcgat
      541 gttgcctgcg caaccgccga actcgcggcg catctcgggc acgaggaagg cgacgttgag
      601 gatgtggatc tggtccggga ggatgtgatt ccggaagtgg tccttgaaga ccatgatgtt
      661 gtcgaaggcg atcgagccgc agatcagcgt tttcattggg cgctcgggtc cttgcggacc
      721 atcagggata aaagaggtag aggcggtaac cgacggcgcc gatttccgag acgtcgatcc
      781 acagccgcag attggattcg cccaggggcg gaaagccgcg ggcgattcgc gcgcggcccg
      841 cgtcgccgga atcggccaga tagtcaccgg ggaagagtac gcgacggacc agcggtcggt
      901 cggcggagtc ggtcagcgtc agctccaggt tcgggtaagc ctgcgcgtag ctcgcagagt
      961 tgcgcagcgt cgcgtcgagc agcacgaggc cgggccgcgc cgggtcggtc tgaagcgtcg
     1021 acgattcgat cgcgacgaag gtggtctcgc gcggcagatc gatcgtgcag tggaactgca
     1081 ggcaaagtgc ttcgaggaag ggccgggcgg ccggcaggtg ggcggcgagg tcggttcgcc
     1141 agtgataggc gccctgcaga agcagcgcga ccagcgcgac gaggctgacg accggccaga
     1201 gcgtccgcgc aaccggcgac atctcacccg ccggcgccgc aatcgagatg ctgcccgacg
     1261 acagcgggct ctcggcccag cggttgtagc ccggcgccgt acggatctcg cgaacggttt
     1321 gggcgaggtc ctcgcgggac tctgcggctt cggcccctgc ttcgggcgcc gcttcgctgg
     1381 cgatgggagg tgttgccggt tcgggatctg ccggggcttc cgccgccggc actgccggta
     1441 ccgcgacgcc cgcatcctgg ttgtcgccga tgacctcggc agcaggcggc acagctgcct
     1501 cggcctcgcg ttcggcggcc acgttggtca gcgcccgatt gattttctcg ggccactgca
     1561 aatcgagttt cggcgcaatc gagtcgggcg aatcctcgat caggtgttcg aggacattga
     1621 ataccgcctt gcacgagccg cagcggacct tgccgctctt cgccttcatc tgcttgggcg
     1681 taacgcggaa actggtgtcg cagtgtgggc agcgggcgat gacggccatc agcttgctcc
     1741 cggcatcggc atcaacattg gcttctgccc ggccaggcag acccagccct cgcggctgcc
     1801 ggcgacctcg agcgcaatcc acggcgcgta gttggcgatc agctcatcgg cctgctcgac
     1861 gagaattccc gacagggcca gccggccgcc atcgtcaacg tgggcacaca gagccggcgc
     1921 cagcgccttc agcgggttgg cgaggatgtt ggcaacgacg atgtggtagc gtccgtcgtg
     1981 cccggcgctg accgactcga aacggatggc gaccccgttg cgcgccgcgt tatcgcgtgc
     2041 ggcggtgacc gcctgcgggt cgatgtcggt gccgacgacc gggccggcac cgagcttggc
     2101 tgccgcaatc gcgaggattc ccgacccgca gccgtagtcg aggacatcta cgccgcggtc
     2161 gagatgctcg accagccatt cgaggcacag ccgcgtcgtc gggtgcgaac cggtgccgaa
     2221 ggccaggccc ggatcaagtt cga
//
LOCUS       DATDAA010000254        10561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_193404,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000254 DATDAA010000000
VERSION     DATDAA010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10561
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193404"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VIS73_08000"
     CDS             complement(<1..1167)
                     /locus_tag="VIS73_08000"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY5763136.1"
                     /translation="MAEIRNYTLNFGPQHPAAHGVLRLVLELDGEVVQRADPHIGLLH
                     RATEKLAETRTWVQSVPYMDRLDYVSMMCNEHAYCMAVERMLNIEVPIRAQYIRVMFD
                     EVTRILNHLLWVGCHALDVGAMAMVLYAFREREDLMDCYEAVSGARLHAAYYRPGGVY
                     RDLPDSMPQYRVSKWKNEAQVKAFNEARQGSMLDFLEDFTNRFPKLLGEYHTLLTDNR
                     IWKQRLVNVGVVSPERALQLGFTGAMLRGSGIAWDLRKKQPYEVYDQMDFDIPVGVNG
                     DSYDRYLVRMEEMAQSNRIIKQCIAWLREDSAKGAGPVITDNHKVAPPSREAMKSNME
                     ELIHHFKLFTEGIHVPPGEAYAAVEAPKGEFGVYFISDGANKPYRMKLRAPGFIH"
     gene            complement(1160..1765)
                     /locus_tag="VIS73_08005"
     CDS             complement(1160..1765)
                     /locus_tag="VIS73_08005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY5763137.1"
                     /translation="MSAKLERLSAALEASLGDKVLSKVTALGELTIEVSATDYREIML
                     ALRDDESLAFDELIDLCGVDYSTYGDGAWAGKRFAVVVHLLSVAKNWRLRVRVFADDD
                     GFPSVDSVMSVWSAANWFEREAFDLYGIVFPGHDDLRRILTDYGFMGHPFRKDFPISG
                     HVEMRYDPEEQRVVYQPVTIEPREVTPRIIREENYGDSVNG"
     gene            complement(1783..2259)
                     /locus_tag="VIS73_08010"
     CDS             complement(1783..2259)
                     /locus_tag="VIS73_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY5763138.1"
                     /translation="MSIEGILQEGFVTTTADKLINFMRTGSLWPMTFGLACCAVEMIH
                     AGCSRYDLDRFGVVFRPSPRQSDVMIVAGTLTNKCAPALRKVYDQMPEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIIQLQNKIRRTNTIAR"
     gene            complement(2264..2620)
                     /locus_tag="VIS73_08015"
     CDS             complement(2264..2620)
                     /locus_tag="VIS73_08015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY5763139.1"
                     /translation="MLENYFPILMFILVGIAMGVIPVLLGWFAAPRRPDPEKLSPYEC
                     GFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWALVLNEIGMFGFVSMMIFLAIL
                     VVGFIYEWNKGALEWE"
     gene            complement(2721..2805)
                     /locus_tag="VIS73_08020"
     tRNA            complement(2721..2805)
                     /locus_tag="VIS73_08020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2769..2771),aa:Leu,seq:gag)
     gene            complement(2898..3269)
                     /gene="secG"
                     /locus_tag="VIS73_08025"
     CDS             complement(2898..3269)
                     /gene="secG"
                     /locus_tag="VIS73_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY5763140.1"
                     /translation="METLISLALVVHLIVALVLIGLILLQHGKGADMGAAFGSGASGS
                     LFGASGSANFLSRTTGVLATVFFISSLALAYMMAAQPRATGSVMEKSVESPPVLTPEK
                     TTDGAASVPPSESRAKDIPTR"
     gene            complement(3273..4034)
                     /gene="tpiA"
                     /locus_tag="VIS73_08030"
     CDS             complement(3273..4034)
                     /gene="tpiA"
                     /locus_tag="VIS73_08030"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY5763141.1"
                     /translation="MRKPFVAGNWKMNGSLAANGALLQAVRAGAGSLSGVEVAVCPPA
                     VFLAQVAQMLDGSTIAWGSQNVSEHASGAYTGELSASMLREFGCSFAIVGHSERRALF
                     GEDDRVVAEKAASALAGGLTPIVCVGETLSERDSGATLSVVERQLAAVIERIGTVDLA
                     RIVVAYEPVWAIGTGRTATPEQAQEVHAAIRRQLSRASADAAAQVRILYGGSVKPANA
                     AEIFSMTDVDGGLIGGASLSAADFMAICTAAAQKS"
     gene            complement(4133..5497)
                     /gene="glmM"
                     /locus_tag="VIS73_08035"
     CDS             complement(4133..5497)
                     /gene="glmM"
                     /locus_tag="VIS73_08035"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY5763142.1"
                     /translation="MTRRYFGTDGVRGKVGDSPITPDFVMRLGFAAGQVLVSREALPA
                     GDHPAVLIGKDTRISGYMLEAALEAGFAAAGVDVSLVGPMPTPAVAYLTRALRLQAGV
                     VISASHNPFYDNGIKFFSSDGNKLPDEVEQAIERAVEETMGQPLGCVPSERLGKARRV
                     GDAAGRYIEFCKSTFPNDLDLRGLKIVVDCAHGAAYHIAPHVFHELGADVVAIGAAPD
                     GLNINAAVGATHVEALQQAVAANDADLGIALDGDADRVMMVGRSGRVFDGDLLLYAII
                     KGRHGSETVDGAVGTLMSNLALENALKGMGVGFARAAVGDRYVLELMKQKGWRFGAEN
                     SGHIICLDRHTTGDGIVAALQVLTALRQADQSLDDFLADISLYPQRLINVPMAKGAPW
                     RDSKPIASAVSAVEAEMGSRGRVLLRPSGTEPVLRVMVEGQDRGEVDRAAEGLAGEIK
                     AHLA"
     gene            complement(5502..6272)
                     /gene="folP"
                     /locus_tag="VIS73_08040"
     CDS             complement(5502..6272)
                     /gene="folP"
                     /locus_tag="VIS73_08040"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY5763143.1"
                     /translation="MGIVNLTPDSFSGDGVGASTDAALVQARRLIAEGADILDLGAES
                     SRPGAQPIADEEEIARLLPVVTALLEDGVAISVDTCKPAVMAAVLNAGVDMINDIRGF
                     ESAASRAALRGYNCGVCAMHMRGDPATMQQHPEYRDVVGEVSAYLAARIDDLRQAGVA
                     GERIVVDPGFGFGKRLPHNVALFRAVDGWAKAGWHVLVGVSRKSMLGEITGRSVDGRL
                     VASTAAALLAAQRGARILRVHDVGATRDALAVCEALGG"
     gene            complement(6364..8244)
                     /gene="ftsH"
                     /locus_tag="VIS73_08045"
     CDS             complement(6364..8244)
                     /gene="ftsH"
                     /locus_tag="VIS73_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY5763144.1"
                     /translation="MNNMFKNLAIWMVIGLVLMTVFNQFNARQQTQSVMEYSQFIQEV
                     EEGRIAKVVMEGRILKAVTTDGRRITSYAPPDLWLVSDLLKYGVSIEARPEEEQSMLM
                     TIFVSWFPMLLLIGVWVFFMRQMQGGGRGGAFSFGKSRARMLDESNNSITFADVAGCD
                     EAKEEVSELVDFLRDPSKFQKLGGRIPKGVLMVGSPGTGKTLLARAIAGEAKVPFYSI
                     SGSDFVEMFVGVGAARVRDMFEQAKKHAPCIIFIDEIDAVGRQRGAGLGGGNDEREQT
                     LNQLLVEMDGFEGHVGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREQILK
                     VHMRKVPVAPDADAGVIARGTPGFSGADLANLVNEAALFAARANKRLVDMEDFEKAKD
                     KIMMGAERRSMVMTEEEKLNTAYHESGHTVVAKLVPKSDPVHKVTIIPRGRALGLTMQ
                     LPEQDRYAYDRQYLLSRIAVLFGGRIAEELFMNQMTTGASNDFERATQMAREMVTRYG
                     MSELLGPMVYGENEGEVFLGRSITTHKNVSEATMQKVDSEIRRIIDEQYALARDLLEE
                     HREKVEAMTRALLEWETLDSDQIDDIMAGGPPRPPKPVQSTPVKPPDEAPGAEPNAPA
                     AA"
     gene            complement(8293..8922)
                     /locus_tag="VIS73_08050"
     CDS             complement(8293..8922)
                     /locus_tag="VIS73_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEY5763145.1"
                     /translation="MSRSKTSKAWMNQHVNDVYVQRSKRDGWRSRAAYKLLEIDRQDN
                     LLRPGTQVVDLGSAPGGWSQVAASKVGERGRVIALDRLEMHPISGVTFIQGDFTEDST
                     LAALETALAGAPVDLVISDMAPNLTGVAATDQARAAYLNELALEFAGNWLQPDGALLV
                     KAFHGRDFESFRNEMRRRFVVFVERKPEASRDRSAEVYLLGKGLKAVAQ"
     gene            8955..9302
                     /locus_tag="VIS73_08055"
     CDS             8955..9302
                     /locus_tag="VIS73_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HEY5763146.1"
                     /translation="MLSITAEERRALRAQAHALKPVVMIAENGLSASVMQEIDRSLAA
                     HGLIKIRVFSDDRELRETQLEEICTAHGAAPVQHIGKLLVIWRPLVDDGGKKPVRPRA
                     PRQTKRSFQGGER"
     gene            complement(9313..9789)
                     /gene="greA"
                     /locus_tag="VIS73_08060"
     CDS             complement(9313..9789)
                     /gene="greA"
                     /locus_tag="VIS73_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY5763147.1"
                     /translation="MSKIPLTVSGAEKLRAELHRLKTIERPSVIAAIAEARAHGDLSE
                     NAEYDAAKERQGFIEGRIQEIEGKLGNAQIIDPRALDADGRCVFGATVDLEDAESGDT
                     VTYQIVGDDEADIKAGKISVGSPVARALIGKFAGDLAQVQAPGGMREYEILDVRYI"
     gene            complement(9839..>10561)
                     /gene="carB"
                     /locus_tag="VIS73_08065"
     CDS             complement(9839..>10561)
                     /gene="carB"
                     /locus_tag="VIS73_08065"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436684.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEY5763148.1"
                     /translation="VYVLEVNPRASRTVPFVSKATGLPLAKIAARCMAGRSLASQGVT
                     REIIPPYYSVKEAVFPFAKFPGVDSILGPEMKSTGEVMGVGYSFAEAFVKSQLAAGIR
                     LPASGRVFISVKDSDKAKAVEIARDLHEQGFQLIATRGTAAAIAATGLPVTAVNKVTE
                     GRPHVVDMIKNNEIALIINTVDERRQAIADSTSIRTSGLAQRVTVYTTIWGAEAAAEG
                     MRRGGELVVYPIQDLHASLSPA"
BASE COUNT         2074 a         3405 c         3135 g         1947 t
ORIGIN      
        1 atggatgaag cccggagccc tcagcttcat gcggtagggc ttgttggcac cgtcggaaat
       61 gaagtacacg ccgaactcgc ccttgggcgc ctcgacggcg gcataggcct cgcccggcgg
      121 cacgtggatg ccttcggtga agagcttgaa gtggtgaatc agctcttcca tgttggactt
      181 catcgcttcg cgcgacggcg gcgcaacctt gtggttgtcg gtaattaccg ggccggcgcc
      241 ttttgccgaa tcttcgcgca gccaggcaat acactgcttg ataattcgat tcgattgcgc
      301 catctcttcc atgcggacca gatagcgatc gtaggagtca ccgttgacgc cgaccgggat
      361 gtcgaaatcc atctggtcgt agacttcata gggctgcttc ttgcgcaggt cccaggcgat
      421 gcccgacccg cgcagcatcg cgccggtaaa gccgagctgc agtgcccgct cgggcgacac
      481 gactccgaca ttgaccagtc gctgcttcca gatgcggttg tcggtgagca acgtgtggta
      541 ttcgccgagc agcttcggga atcgattggt gaagtcctcg agaaaatcga gcatcgaacc
      601 ctggcgcgcc tcattgaagg ccttgacctg cgcttcgttc ttccacttgg aaacgcggta
      661 ctgcggcatg ctgtcgggca ggtcgcggta gacgccgccg ggccggtaat acgccgcgtg
      721 cagtcgcgcg ccggagaccg cttcgtagca gtccatcagg tcttcgcgct cgcggaatgc
      781 gtaaagcacc atcgccatcg caccgacgtc aagcgcgtgg caaccgaccc acaacagatg
      841 gttgaggatc cgggtcacct catcgaacat cacgcggatg tactgggcgc ggatcggcac
      901 ttcaatgttg agcatgcgct ccactgccat gcagtaggcg tgctcgttgc acatcatcga
      961 tacgtagtcg aggcggtcca tgtaggggac cgactgtacc caggtgcgcg tttcggccag
     1021 cttctcggtg gcacggtgca agagtccgat gtgcgggtcg gcgcgctgca cgacctcgcc
     1081 gtcgagctcg agaaccaggc gcaacacgcc gtgcgccgcc gggtgctgtg gaccgaagtt
     1141 cagcgtgtaa ttgcgaatct cagccattga ccgaatcccc gtagttttcc tcgcgaatga
     1201 ttcgcggcgt aacctcgcgc ggctcaatcg ttaccggctg gtagacgacc cgctgctcct
     1261 ccgggtcgta gcgcatctca acgtgcccag aaatcgggaa gtccttgcgg aacggatgcc
     1321 ccatgaagcc gtagtcggtc aggatgcgcc gcaggtcgtc gtggccgggg aagacgatgc
     1381 cgtagagatc gaatgcctcg cgctcgaacc aattggcggc gctccacacg gacatcaccg
     1441 agtcgaccga cgggaatccg tcgtcgtcgg caaagacgcg aacacgcaag cgccagttct
     1501 tcgctaccga cagcagatgc acgaccactg cgaaacgctt gccggcccaa gcaccgtcgc
     1561 cgtaggtcga atagtcaacg ccgcagagat cgatgagttc gtcgaaggcg agcgactcat
     1621 cgtcgcgcag cgccaacatg atctcgcgat agtcggtggc ggatacctcg atggtcagct
     1681 cgccgagagc cgtcaccttc gagagcacct tgtccccgag cgacgcttcg agggcagcag
     1741 agagtctttc gagttttgcg gacatgcctg attccccgtc gctcagcgag cgatcgtgtt
     1801 agtgcggcga attttgttct gcagctggat gattccgtag agcagcgcct cggcggtcgg
     1861 cggacagccg gggacgtaga tatcgaccgg aacgatgcga tcacagccgc ggacgaccga
     1921 atacgaatag tggtagtagc caccgccgtt ggcgcaggaa cccatcgaaa tgacccagcg
     1981 cggttcgggc atctggtcgt aaaccttgcg cagcgccggc gcgcacttgt tggtcagggt
     2041 tccggcaacg atcatcacat ccgactggcg cgggctgggg cgaaagacga cgccgaatcg
     2101 atccaggtca tagcgcgagc aaccggcgtg aatcatttcg acggcacaac aggcaaggcc
     2161 gaaggtcatc ggccacagcg agccggtgcg catgaagttg atcagcttgt cggccgtcgt
     2221 ggtgacgaaa ccttcctgga ggatgccttc gatactcatg atttcactcc cactccagcg
     2281 cgcccttgtt ccactcgtag atgaagccga ccaccaggat cgccaggaag atcatcatcg
     2341 agacaaaacc gaacatgccg atctcgttaa gcacaagcgc ccagggaaac aggaaggcaa
     2401 tttccagatc gaacaggata aagaggatgg cgacgaggta gtagcgcacg tcgaacttca
     2461 tgcgcgcatc ctcgaaggcc tcgaatccgc actcgtacgg ggagagtttt tccgggtctg
     2521 gacgtcgcgg agccgcaaac cagccaagca agaccgggat gacacccatt gcgataccga
     2581 ccaagatgaa catgaggatg gggaagtagt tttctagcat cgtcgcgtcg ccgattggcc
     2641 tgggttcgtt tgaacgccgc ccccgtccga aacaactgcg attcgaacgg aacacggcaa
     2701 gagccgcact tccggattac tggtgccgac ggcgagactc gaactcgcac agctttcgcc
     2761 actaccccct caagatagcg tgtctaccaa tttcaccacg tcggcacttc gcactgcgta
     2821 gcggattgcg caaggaggga aaaccgcaat ccgcctaact acaaaatctg attatttgct
     2881 gctgcgcgcc aaattgctca gcgcgtcggg atgtcctttg cccgcgactc cgaaggcggc
     2941 acgctcgccg cgccatctgt tgtcttttca ggtgtcagaa caggcggcga ttctaccgat
     3001 ttctccatca cgctgccagt cgcacgcggc tgtgcagcca tcatgtaggc gagcgcgaga
     3061 ctgctgatga agaagaccgt ggcgaggacg ccggttgtcc gcgaaagaaa gtttgccgac
     3121 ccgctcgcgc caaaaaggct acccgaggcg ccgcttccga aagccgcgcc catgtcggca
     3181 ccctttccgt gttgcagcag gatcagtccg ataaggacca gcgcaacgat caggtgaact
     3241 acgagggcca gcgaaatcaa agtttccatt tatcagctct tttgggcggc tgcggtgcag
     3301 atcgccataa aatcggcggc ggaaagcgat gcaccaccga tgagtccgcc atcgacatca
     3361 gtcattgaaa agatctcggc ggcattggca ggtttgacgc tgccaccgta aagaatccgt
     3421 acttgggcag cggcgtcagc cgatgcccgt gaaagctggc ggcgaatcgc cgcatggact
     3481 tcctgcgcct gctccggcgt cgcggtccga ccggttccaa tcgcccagac cggctcatag
     3541 gcaacgacga tgcgcgccag gtcgaccgtg ccgatccgtt cgattacggc ggccaactgc
     3601 cgctcgacaa ccgagagcgt cgcgcccgag tcacgttctg agagcgtttc gccgacacag
     3661 acaattggcg tcaatccgcc ggccagcgca ctcgcagcct tttccgcgac aacccgatca
     3721 tcttcaccga acagtgcacg gcgctcggag tgaccgacga tcgcaaagga acaaccgaac
     3781 tcgcgcagca tcgatgcgct gagctctccc gtataggcgc cactggcgtg ctcggagaca
     3841 ttctgcgagc cccaggcaat cgtcgaaccg tcgagcatct gggccacctg cgccaggaaa
     3901 accgctggcg gacagaccgc cacttcgacc cccgacaggg acccggcgcc cgcacgaacg
     3961 gcctgcagca gcgcgccatt ggcggcgagg ctgccgttca ttttccagtt gccggcgacg
     4021 aagggtttgc gcatcgattg ggggtccaaa aaagccgcga gattatagct gcgacgacat
     4081 ttccggtcaa acccaaaggc cgcctgaatt cgacgacaac gcgatccccc gctcaggcca
     4141 gatgcgcctt gatctcgccg gccagccctt cggcggcgcg gtcaacctcg ccccgatcct
     4201 gcccctcaac catcacgcgc aacaccggtt cggtccccga cgggcgcagc agtacccgac
     4261 cgcgactgcc catctcggcc tcgaccgccg acaccgcgga ggcaatcggc ttcgaatccc
     4321 gccagggagc acctttcgcc atcggtacgt tgatcaggcg ctgcgggtat agcgaaatat
     4381 cggcgagaaa atcgtccagg ctctgatccg cctggcgcag cgccgtcagt acctgcaggg
     4441 cggcaacgat gccgtcgccg gtggtatgcc ggtcgaggca gatgatgtgc ccggaattct
     4501 cggcgccaaa gcgccaaccc ttctgtttca tcagttcgag cacatagcgg tcgccgacgg
     4561 cggcgcgggc gaagccgaca cccatgccct tcagcgcgtt ctccagcgcc agattggaca
     4621 tcagcgtacc gaccgcaccg tcgaccgtct cgctgccatg acgccccttg atgatcgcgt
     4681 agagcaacaa atcgccgtca aagacgcgtc cggagcggcc gaccatcatc acgcggtcgg
     4741 cgtcgccgtc gagtgcgata ccaagatcgg catcattggc ggccaccgcc tgttgcagag
     4801 cctcgacatg cgtcgcaccg accgccgcgt taatgttcaa accgtccggt gccgcgccga
     4861 tcgccacgac gtcggcgccg agttcgtgaa agacatgcgg cgcgatgtga taagcggcgc
     4921 cgtgcgcaca atcgacgacg atcttgaggc cgcggagatc gaggtcgttg ggaaacgtgc
     4981 tcttgcagaa ctcgatatag cgtccggcag catcgccaac ccgcctcgcc ttgcctagtc
     5041 gctcggatgg gacgcagccc agcggctgcc ccattgtttc ctcgaccgcg cgctcaatcg
     5101 cctgctcgac ttcgtcgggc agcttgttgc catccgagga aaaaaacttg atgccgttat
     5161 cgtagaacgg attatgggag gccgagatca ccacgcctgc ctgcaggcgc agggcgcgcg
     5221 tcaggtaggc aaccgctggc gtcggcattg gcccgaccag actgacatca acgcctgccg
     5281 cggcgaaacc ggcttccagc gctgcttcca gcatgtagcc ggaaatccgc gtgtccttgc
     5341 caatgagcac ggcaggatgg tcacccgccg gcaatgcctc gcgggagacc aacacctgcc
     5401 ccgccgcgaa tcccaggcgc atgacgaaat ccggcgtgat cggcgaatcg ccgaccttcc
     5461 cccgaacacc atcggttccg aaatacctgc gtgtcactgt ttcatcctcc caaagcttca
     5521 caaacggcga gcgcgtcgcg cgtcgccccg acatcgtgca cccgcagtat ccgggcaccg
     5581 cgctgagccg caagcagcgc cgcggcagta ctcgcgacga gccggccgtc caccgagcgt
     5641 cctgtgattt cacccagcat cgacttgcgg gagacgccga cgagcacgtg ccagcccgcc
     5701 ttcgcccacc catcaacggc cctaaaaagc gcgacgttgt ggggtagccg cttgccgaaa
     5761 ccaaaaccgg gatcgacgac aatccgctcg ccggcgacgc cagcctgacg gagatcgtcg
     5821 attcgcgcgg caagatacgc cgagacttcg ccaacgacgt cacggtactc gggatgctgc
     5881 tgcatcgtcg ccgggtcacc gcgcatgtgc atggcgcaca cgccgcagtt gtagccgcga
     5941 agcgcggcgc gcgatgccgc gctctcgaag ccgcggatgt cgttgatcat gtcgacgccg
     6001 gcattgagca cggcagccat caccgccggc ttgcaggtat cgaccgatat cgctactccg
     6061 tcctccagca aggccgtaac gaccggaagc agacgcgcaa tctcctcttc gtcggcaatc
     6121 ggctgcgctc ccgggcgtga tgactcggcg ccgagatcga ggatgtctgc gccttcggca
     6181 atgaggcggc gcgcctggac aagtgcggca tccgtgcttg cgccgacacc gtcacccgag
     6241 aacgaatcag gcgtcaggtt gacgataccc atcagcaacg gccgttgccc cgaaaacgag
     6301 aaacggccgc agacgaaagt cgtcgcggcc gcttccggcg ggtcgtgccg aggcgtcaat
     6361 tgatcaggcg gctgccggcg cgttgggttc ggcgccaggc gcctcgtccg gcggtttcac
     6421 cggggtcgac tgcactggct tgggtggacg cggcggaccg ccggccatga tgtcgtcgat
     6481 ctgatcggaa tccagagttt cccattcgag cagtgcccgg gtcatcgcct ctaccttttc
     6541 ccggtgttct tccagcaggt cacgcgccaa agcgtattgt tcatcgataa tccgccggat
     6601 ctcggagtcg accttctgca tcgtcgcctc ggaaacgttc ttgtgggtcg tgattgagcg
     6661 gccgaggaat acctcgcctt cgttctcgcc atagaccata ggcccgagca attccgacat
     6721 gccgtagcgc gtaaccatct cccgcgccat ttgggttgcc cgctcgaaat cgttcgaggc
     6781 gccggtagtc atctgattca tgaacaattc ttcggcaatt cgcccgccaa agagcacggc
     6841 gatccggctc agcaggtact gccggtcgta ggcgtagcga tcctgctcgg gaagttgcat
     6901 ggtcaaacca agcgcccgcc cgcgcgggat gatggtgacc ttgtgcaccg ggtcggactt
     6961 cggcaccagc ttggccacta ccgtatggcc agactcgtga tacgcggtgt tgagtttttc
     7021 ttcctcggtc atgaccatcg agcgccgctc ggcacccatc atgatcttgt ccttggcttt
     7081 ctcgaagtcc tccatgtcga ccagacgctt gttcgcacgt gcggcgaaca gcgccgcctc
     7141 gttgacgagg ttggccagat cggcacccga gaatcccggc gtaccgcgcg caatcacgcc
     7201 ggcgtcagca tcgggcgcta ccggcacctt gcgcatgtgg accttgagaa tctgctcccg
     7261 gccccggata tcgggcagcg gcacgactac ctgacgatcg aaacgtccag ggcgcagcag
     7321 ggccggatcg agaacgtccg gacggttggt cgcggcgatg acgatgacgc ccacgtgacc
     7381 ctcgaaaccg tccatctcga cgagcaactg gttgagcgtc tgctcgcgct cgtcgttgcc
     7441 gcccccgagg ccggcgccac gctggcgacc gaccgcatcg atctcgtcga tgaagatgat
     7501 gcaaggcgcg tgcttcttcg cctgttcgaa catgtcgcgg acgcgcgcag caccgacacc
     7561 gacaaacatc tcaacgaaat cggaacccga aatggaatag aacggcacct tggcctcgcc
     7621 ggcaatggcc cgcgcgagca atgtcttgcc ggtaccgggg gagccgacca tcagcacacc
     7681 cttgggaata cggccgccga gcttctgaaa cttcgacggg tcgcgtagaa aatcgaccag
     7741 ttcggaaacc tcttccttcg cctcgtcaca acctgcgaca tcggcgaagg ttatcgagtt
     7801 attcgactcg tcgagcatgc gcgcgcggct cttgccgaac gaaaaggcgc cgccgcgacc
     7861 accgccctgc atctggcgca tgaaaaacac ccagacgccg atcagcagca gcatcgggaa
     7921 ccagctgacg aagatggtca tcagcatcga ctgctcttcc tcgggccgcg cctcgatcga
     7981 cacaccgtac ttgagcaggt cagaaaccag ccagaggtca ggcggggcgt aggaagtaat
     8041 gcgccgtcca tcggtcgtta ccgccttgag gatacggcct tccatgacga ccttggcgat
     8101 ccggccctcc tccacttcct ggatgaactg ggagtactcc ataacgctct gcgtctgttg
     8161 ccgggcgttg aactggttga acacggtcat cagcacgagg ccaataacca tccagatcgc
     8221 cagattcttg aacatgttgt tcacgtcgat atgcccttcg ttacgtcgtt tcgtaaaagt
     8281 gattctagcg cgtcactgcg ccacagcctt taaacctttg cccagaagat agacttcggc
     8341 acttcgatca cgcgatgctt cgggttttct ttcgacgaaa accacgaatc gccttcgcat
     8401 ttcgtttcgg aatgattcga agtcccgtcc gtgaaaggct ttgaccagca gcgctccgtc
     8461 tggttgcagc cagtttccgg caaattcgag cgcgagttcg ttcagatagg ccgcgcgcgc
     8521 ctgatcggtg gcggctacac ctgtcagatt gggggccatg tccgaaatca caaggtcaac
     8581 gggcgcccca gcgagtgctg tttctagcgc tgccagggtg gaatcctcgg tgaaatcgcc
     8641 ctgaatgaag gtcacgccgc tgatcggatg catctccagc cgatcaagcg cgatgacgcg
     8701 cccgcgttcc ccgaccttcg atgcagccac ctggctccat cctcccggcg ccgaccctag
     8761 atcgacgacc tgcgtaccgg gccgcagcag attgtcctgg cggtcgatct cgagcagctt
     8821 gtaggccgcc cgcgagcgcc aaccgtcacg cttggagcgc tgcacgtata cgtcattgac
     8881 gtgctgattc atccacgcct tgctggtctt gcttcggctc attggctaga attcgcgctc
     8941 actggagaac cccaatgctt tcaattactg ccgaggagcg ccgcgcactg cgggcccagg
     9001 ctcacgcact caaacccgtt gtcatgatcg cggaaaacgg cctgagcgcc tcggtgatgc
     9061 aggaaatcga ccgtagtctg gcggcgcacg gcctgatcaa gatccgcgtc ttcagcgacg
     9121 atcgggagct tcgtgaaacg cagcttgaag agatttgtac cgctcacggc gccgcgccgg
     9181 tgcagcacat cggcaagttg ctggtgatct ggcggccgct ggttgacgat ggcggaaaaa
     9241 agccggtgcg tccgcgcgcg ccgcggcaaa ccaagcgcag cttccagggc ggcgaacgct
     9301 gaacgcccgg tttcaaatgt aacgaacgtc gagaatttcg tattcacgca tgccgcccgg
     9361 agcctgcacc tgcgccaggt caccggcgaa cttgccgatc agtgcgcgcg ccaccggtga
     9421 tccaaccgag atcttgccgg ccttgatgtc agcctcgtcg tcgccgacga tctggtaggt
     9481 cacggtatcg cccgattcgg cgtcctctag gtctacggtc gcaccgaaca cgcaacggcc
     9541 atcggcatcg agcgcccgcg gatcgatgat ctgcgcgttg ccgagcttgc cctcgatctc
     9601 ctgaatgcgt ccctcgatga agccctgccg ctccttggca gcgtcgtatt cggcattctc
     9661 cgatagatca ccgtgggcac gagcttcggc gatcgcggca atcaccgacg gacgttcgat
     9721 ggtcttcagt cgatgcagtt cggcgcgcag tttctcggca ccggatacgg tcagtggaat
     9781 cttgctcatg acgtctgtgc aaccagcctt tctgctgaat cggtcaaggt cagccgggct
     9841 aggccggcga cagcgacgcg tgtagatcct gaatcggata gacgacgagt tcgccaccgc
     9901 gccgcatgcc ttccgcggct gcttcggcgc cccagatcgt ggtgtagacg gtcacacgct
     9961 gcgcaaggcc cgaggttcgg atcgacgttg aatcggcgat cgcctgccgg cgctcgtcga
    10021 cggtgttgat gatcagggca atctcgttgt tcttgatcat gtcgaccaca tgaggccgac
    10081 cctcggtcac cttgttgacc gccgtgaccg gcagaccggt cgccgcgatc gccgccgccg
    10141 tgccacgtgt cgcgatcaac tggaatccct gctcgtgcag gtcacgggct atctcgacag
    10201 cctttgcctt gtcggaatcc ttgacgctga tgaagacacg accgctggcc ggcagcctaa
    10261 ttccggctgc cagctgcgac tttacgaagg cctcggcaaa gctgtagccg acgcccatga
    10321 cctcgccggt tgatttcatc tcgggcccaa gaatgctgtc cacgccgggg aacttggcga
    10381 aggggaacac cgcctccttc accgagtagt acggcgggat gatctcgcgc gtcaccccct
    10441 gcgaggcaag gctcctgccg gccatgcagc gcgccgcgat cttggccagc ggcagtccgg
    10501 tcgctttcga cacgaagggt accgtgcgcg aagcgcgcgg attcacttcc agtacgtaga
    10561 c
//
LOCUS       DATDAA010000255         2232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_18326,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000255 DATDAA010000000
VERSION     DATDAA010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18326"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..1631
                     /locus_tag="VIS73_08070"
     CDS             297..1631
                     /locus_tag="VIS73_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5763149.1"
                     /translation="MAVGLRASLRQLGERVFSEQDSEQDRLNKTLAIFAAGLMGFGAM
                     LWLVIYQAMGIKFSATVPFTYIVVSAASLGLFLWNRNFALYRFIQTTLFLFIPFVMQW
                     SIGSYVSSSGVMLWALLAPVGVLMFQGPRESLPWFFAYLVMTAVSGFFDYFLTEGTDS
                     GISAQSIAVFFALNFAAMSTIIYLLISFFVRQRDQLQARVDDQYRLLQDEQEKSERLL
                     LNILPGPIAERLKANQTTIADGFSDVTVMFADIINFTRLAEEMPPKFMVTLLNEVFSR
                     FDELAEKHGLEKIKTIGDAYMVAGGLDEHHDRLGSEGVERDYCSAVCGMAIDMRDYME
                     ALSGIRQARLQIHVGIGTGPVVAGVIGMKKFIYDLWGDTVNIASRVTEHAGPSTILVD
                     ATTYRRARNRYDFDGPSDIQVKGKGSLTVYRLVGPKPEAALGTALPQAGSAS"
     gene            complement(1729..>2232)
                     /locus_tag="VIS73_08075"
     CDS             complement(1729..>2232)
                     /locus_tag="VIS73_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamine synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HEY5763150.1"
                     /translation="LTRARFVAGDQAIGNAFEALRVKILRQEREPETLRREVLAMRQK
                     LLDGHPNRTPLFDLKHDRGGLIDVEFVVQYLVLGHAHRHPELTGNIGNLALLKLAARL
                     GLIAPERAQAAHDAYRRLRQLQHGLRLRGERYARIEPEAIAGERQAVEKLWADMFGEA
                     PSGVAGT"
BASE COUNT          369 a          689 c          727 g          447 t
ORIGIN      
        1 gtggtggcgt tgcgccacgg cacgaggcgg ccgtcatacc agatctgccc gtcacggtcg
       61 gccatcgagg acatttcgga acccatcgag gaaaaggcgc attttagcgc atgtcgcaag
      121 ctctaccgaa gcccgctcgt gccctgcacc cgaggtgccg ttgtgcggat gtcatgccgg
      181 ggtggaattt gccacacccg cttggtggta aactccgccc ccactcagta gaatgccctg
      241 gttaccgcag ccgctgcttg tgcgccagcg tgattgcggg tcaacaggga gcgttcatgg
      301 cggtcggttt gcgggcgtcg ctgcggcagc tgggcgagcg cgtgttcagc gagcaggaca
      361 gcgagcagga tcggttaaac aagacactcg cgattttcgc ggctggcctg atgggctttg
      421 gcgcgatgct gtggctcgtc atctaccagg cgatgggcat caagttttcc gcgaccgtgc
      481 ctttcacgta tatcgtggtg tccgcagcct cgctcgggtt gttcctgtgg aaccgcaact
      541 tcgccctgta ccgcttcatc cagacgaccc tgttcctgtt catcccgttc gtgatgcagt
      601 ggagcatcgg cagctacgtg agctcttcgg gcgtcatgct gtgggcgctg cttgcgccgg
      661 ttggcgtcct gatgtttcag gggccccgcg agtcgctgcc atggttcttt gcctacctcg
      721 tcatgaccgc ggtgtcgggg ttcttcgact acttcctcac ggaaggcacc gactcgggca
      781 tctcggccca atcgatcgcc gtgttttttg cgctcaactt cgccgcgatg tccaccatca
      841 tctacctgct gatcagtttc ttcgtgcgcc agcgtgacca gcttcaggcg agagtggacg
      901 accaataccg gctgctgcag gacgagcagg aaaaatcgga gcgtctgctg ctcaatatac
      961 tgccgggccc gattgctgag cgtttgaagg cgaatcagac gaccatcgcc gatggcttct
     1021 cggacgtgac cgtgatgttc gccgacatca tcaacttcac ccgcctggcg gaggagatgc
     1081 cgccgaagtt catggtgacg cttctgaacg aggtgttttc gcgcttcgac gagctcgccg
     1141 agaaacacgg actggaaaaa atcaagacga tcggcgatgc ctacatggtg gcgggcggcc
     1201 tcgacgagca tcacgaccgc ctgggcagcg aaggcgttga acgcgattat tgcagcgctg
     1261 tctgcgggat ggcgatcgac atgcgcgact acatggaggc gctgtccggc atcaggcagg
     1321 cgcggctgca gatccacgtc ggcatcggca cggggccggt ggtcgcgggc gtgatcggca
     1381 tgaagaagtt catctacgac ctctggggcg acacggtcaa cattgcgagt cgggtcaccg
     1441 agcatgccgg gcccagcacc atactcgtcg atgccaccac gtatcgtcgg gcgcgcaacc
     1501 gctacgactt cgacggcccc tccgacatcc aggtgaaggg caagggcagt ctgaccgtct
     1561 accggctggt cgggccaaag ccggaagcgg cgctcggtac ggcgttgccg caggcgggct
     1621 ctgctagctg atggtgagtc aggcgctgcg gcaggggttg atgcgcccgt ggtgtagcgt
     1681 gttccggtct cgctttactg tctctaggct cttcgaacgc gtctccggtc aggtgccggc
     1741 cacgccgctc ggcgcctccc cgaacatgtc tgcccagagc ttctcgacgg cctgccgctc
     1801 gcccgcaatt gcttcgggct caatccgcgc gtagcgctcg ccccgaaggc gcaggccgtg
     1861 ctgtagctgc ctcagtcggc ggtaggcgtc gtgtgccgcc tgcgcgcgtt ccggcgcgat
     1921 gagaccgagg cgcgccgcca gcttgagcag ggccagattg ccaatattgc cggtgagctc
     1981 tggatgccgg tgggcatgac cgagcacgag gtactgaacc acgaactcga catcgataag
     2041 gccgccgcgg tcgtgcttca gatcgaagag cggcgtgcgg ttgggatggc catcgagcag
     2101 tttttgccgc atggccagga cctcgcgtcg cagcgtctcg ggctcgcgtt cctggcgcag
     2161 gatcttcacc cgcagggcct cgaacgcatt gccgatcgcc tggtcaccgg cgacgaagcg
     2221 cgcgcgtgtc ag
//
LOCUS       DATDAA010000256        26612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_263022,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000256 DATDAA010000000
VERSION     DATDAA010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 26612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26612
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263022"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="VIS73_08080"
     CDS             complement(<1..1093)
                     /locus_tag="VIS73_08080"
                     /inference="COORDINATES: protein motif:HMM:NF014126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015652 - quaternary ammonium group:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0015697 - quaternary ammonium group
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEY5763151.1"
                     /translation="MSDLPRGIAKQRILINPPVFFSSAILTLAFVIFGVAFPEAAAKG
                     FGVVRDWTMQSAGWFYVLAVASFLIFVIALAVSRFGEIKLGPDHSTPDYSYASWFAML
                     FSAGMGIGLMFFGVAEPIMHYVTPPVGDPQTAEAARQAMRITFFHWGVHAWAIYAVVA
                     LSLAYFSFRHGLPLTIRSSLYPIIGDRINGPIGHAVDVFAVLGTIFGVATSLGFGVLQ
                     VNAGLNHLFDVPTSLTVQVILIAVITGFATLSVSLGLDSGIRRVSELNMILAILLLVF
                     VLVAGPTVFLLQTMVQNTGMYISNLFDMTFNLYAYEPSGWIGGWTLFYWAWWVAWSPF
                     VGMFIARVSRGRTIREFVVGVLLVPVGFTF"
     gene            complement(1399..2058)
                     /locus_tag="VIS73_08085"
     CDS             complement(1399..2058)
                     /locus_tag="VIS73_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763152.1"
                     /translation="MKRQRRALLRFAGASLIGLGGALGAVQRLLAAGLEPVAPGVHRV
                     KGAARINGRPAEPGMPVGPGDTVDTDAGAEIIYVIGKDAYLQREKSSVSFAGEAAVDA
                     LRVITGKLLSVFGKGEKRITTPTATIGVRGTGCYIEAETASTYFCLCYGEVEVMPTAT
                     PNAVHRYRTSYHDKPYEIGDTVQAAMKPAHVINHTDVELTLLESLCGRRPPFFGRDSY
                     Y"
     gene            complement(2135..2557)
                     /locus_tag="VIS73_08090"
     CDS             complement(2135..2557)
                     /locus_tag="VIS73_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEY5763153.1"
                     /translation="MFTPNRDEVRSFFREAWKKQRERAVLEGLEALAADIVAAHPEYQ
                     ALLEGPVEELSREFTPEGGEVNPFLHLSLHLAIAEQLSIDQPPGIAAAYRNLTATREP
                     HEALHVLLDCLGEAVWRASREGRLDHEAYLECIRCKGS"
     gene            complement(2565..3638)
                     /locus_tag="VIS73_08095"
     CDS             complement(2565..3638)
                     /locus_tag="VIS73_08095"
                     /inference="COORDINATES: protein motif:HMM:NF021926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HEY5763154.1"
                     /translation="MTVATALVAANDASPQLAASAVASALERAGITHAQGVLLFLTPH
                     FTRQAATAIHAASRAGNCLQIAGGIVAGVANESSWVFDRPAAVAMAFGAPLRLDAADD
                     GQGPHLAIAGASTLPPAWAALPPRFGTIYSDALSTHATPAWSQGRPATPPIVEVSVGG
                     AETHIAVSTGVRMLGGDWEIDAAHDNVVLTIGGRSAADSLRRALPAEYRTGLPLPLHL
                     LAALPAGEGRGPRHPPRLIPIAALNPDGSLTLGETLPAGQRYTWAIRQPVAAEADMRA
                     TIAALCERSERPADFGFFASCIGRGPYFYGGEDRDWLAIRDGLPGTPFIGVYGSGQVA
                     PYGRANHLLQNAVMLALFHTRPD"
     gene            complement(3635..4306)
                     /gene="nth"
                     /locus_tag="VIS73_08100"
     CDS             complement(3635..4306)
                     /gene="nth"
                     /locus_tag="VIS73_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY5763155.1"
                     /translation="MNRAQRLEFFRRLQEANPEPTTELRYDSPFQLLVAVILSAQTTD
                     RAVNKATEALFRDAPTAAAMAELGEEGIADYIRSIGLYHSKARHLAATCAVLRDQYGG
                     AVPRERAALEALPGVGRKTANVVLNTIFGEPTIAVDTHLFRVANRCGLAPGKTPRAVE
                     DALLKAVPAEYRRHAHHWLILHGRYTCTARRPACARCPVADLCRWPDKSAARAGAAAA
                     KQAPR"
     gene            4423..5064
                     /locus_tag="VIS73_08105"
     CDS             4423..5064
                     /locus_tag="VIS73_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763156.1"
                     /translation="MRGLKLLWVSLAALAVAGCAATRLKSTWVDPTDRGGPLAKVAVL
                     VFHEDDNLRRFAEDQASRTLMGSARIVPGYRLFERPDPDVDKVKDRLIAEGFDGVLMA
                     RTVSVDTQQQYIPPRTMAVPTGPVLVGPFVGAHGFIGYYRHVWGYTYQTLPGYTTEQT
                     TVVVESVLYRLPGGNPVWSAVSETRNPDSAAEVVHDLVTMIGRELADRDLIRR"
     gene            complement(5054..5734)
                     /locus_tag="VIS73_08110"
     CDS             complement(5054..5734)
                     /locus_tag="VIS73_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HEY5763157.1"
                     /translation="MIDRDNLKKVAGSGLWSQNTALVQALGLCPLLAVSTNAINGTTL
                     ALATIIVMMIANIAVAVLRNLIPYEIRIPVFILIIAALVTVIDLLFNAYLHGLYLVLG
                     IFIPLIVTNCIVLARVEAFAAKNPPLIAAFDGIFMGVGLLWTLALLGALRELLGSGTL
                     FGGIDLLVEGWQPLRLLPEDYPGLLLAILPPGGFILLGCLIAAKNVIDARAVRRRAAR
                     PPASAVSA"
     gene            complement(5731..6399)
                     /gene="rsxG"
                     /locus_tag="VIS73_08115"
     CDS             complement(5731..6399)
                     /gene="rsxG"
                     /locus_tag="VIS73_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HEY5763158.1"
                     /translation="MTGLRELTAPAMALRTATILLVFVVVFTALLAGAYRATRPAILA
                     TAAAERMRLISEVLPASVYDNDPLAARISIADDPRLGNAAATIGYRAMRGGKASAVVL
                     EATAPDGYAGRIRLLLAVDRKLRILGVRVLEHRETPSLGDYIEPKKDRNKTRPWITQF
                     DGQSLLTRPLAAWRVRKDGGEFDAYAGATVTPRAVIKTVAKALQFVEAEQATLFDDGA
                     EKRP"
     gene            complement(6396..7409)
                     /locus_tag="VIS73_08120"
     CDS             complement(6396..7409)
                     /locus_tag="VIS73_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HEY5763159.1"
                     /translation="MSTSPFMRQASSVRTVMATVMVALLPAIAAWVALFGAGVLLTLG
                     IASVTALAAEALMLRLRGLPIAPALGDCSALLTAWLLALCLSPLAPWWLVIVGTLFAI
                     IVAKHLYGGLGQNPFNPAMVGFAVCIVSFPALMSQWPAADVDTATQIGWILGGERVDG
                     IIQATPLDHLKTGLRLAEGAASVDQLRADTAVHGYFGGRGWEWVALAYLLGGVFLLAR
                     RIITWQIPFAYLGTVALVSGLFWLWRPEAFATPLFHLLSGGTMLAAFFIATDPVSGAT
                     TPRGKLVFAAGAGLLTVIIRLWGGYPDGVAFAILLMNLCVPLIDMYTQPPVFGATNKG
                     EAP"
     gene            complement(7418..9034)
                     /gene="rsxC"
                     /locus_tag="VIS73_08125"
     CDS             complement(7418..9034)
                     /gene="rsxC"
                     /locus_tag="VIS73_08125"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HEY5763160.1"
                     /translation="MSARLPFASLFSFRGGVKPPAHKEDSCDTPIARAALPERLVVPL
                     HQSIGGTPKPLVKTGDKVLKGQMIGAADGMISAAVHAPTSGRVEAVELAPQPHPSGLS
                     AMAVTIVPDGEERWGERLAVDLKTLTPVDARQRLQDAGVVGLGGAAFPSHAKLDATPG
                     RPLEELVINGAECEPFITCDDRLMRERAEDILRGTALLRDLMQPKRVLIGVEDNKPEA
                     IAALQEAARQIGESGFEIRAVPTLYPAGGAKQLIRVLTGKAIAGTVKRTTDFGVQCFN
                     VATAYTVWRALAHGEPVLSRVVTLAGSVGQPGNWELPIGMSVADAIALAKPKADSDGV
                     LMGGPMMGFRLPDTSAPVVKATNCLIAASPELLPPPPPEMPCIRCGECARACPHELQP
                     FEMYWWSRAKNFGKAQEYALFDCIECGCCSYVCPSNIPLVQYFRFAKGEIWSRERERL
                     AAEGARSRFEFRQFREERDKQEKAERLARAAAAQKAKAAAQAADPEAAAKQAEIAAAL
                     ERTRSQREAAAAAAAAPEPATAPKEETPPS"
     gene            complement(9039..9590)
                     /gene="rsxB"
                     /locus_tag="VIS73_08130"
     CDS             complement(9039..9590)
                     /gene="rsxB"
                     /locus_tag="VIS73_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HEY5763161.1"
                     /translation="MTSAILIMALGAVCLGAALGFASVRFKVKGDPLVEKIDAILPQT
                     QCGQCSYPGCKPYAEAIAKGEADINQCPPGGDEGVRKLADLLGREYKPLSEEHGVEKP
                     KQVAIIDEATCIGCTLCIQACPVDAIVGAAKQMHTIVADQCTGCELCVPPCPVECIRM
                     ESIEESIQTWKWKYPVIEIKRAA"
     gene            complement(9622..10206)
                     /gene="rsxA"
                     /locus_tag="VIS73_08135"
     CDS             complement(9622..10206)
                     /gene="rsxA"
                     /locus_tag="VIS73_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HEY5763162.1"
                     /translation="MTQYFFILLGAVLVNNVVLVKMLGLCPFMGVSKKLETAYGMGAA
                     TTFVLTVATGASFVIDHYLLQPFELEYLRTLSFIVTIAAVVQLTEMVIAKTSPLLQQV
                     LGIYLPLITTNCAVLGVPLLNISHGYGFVESLLFGMGSAIGFSLVLILFGGLRERIEG
                     ASVPAPFRGTAIAMVTAGLMSLAFMGFAGLDRFQ"
     gene            complement(10255..11325)
                     /locus_tag="VIS73_08140"
     CDS             complement(10255..11325)
                     /locus_tag="VIS73_08140"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEY5763163.1"
                     /translation="MLYTMMRSALFALDAEDAHNLTIATLARSPVSRLVNLVSPPLPE
                     TPVRVMGIDFPNRVGLAAGLDKNGAAIDGLAAIGFGFVEIGTVTPRPQPGNAKPRLFR
                     LAGAEAIINRMGFNNDGVERLLANVAAARFPAQGGVLGINIGKNFDTPIERAVDDYRI
                     CLEKVFAAASYVTINISSPNTKNLRDLQRDDALDALLSALKADQQRLTETHGKYTPLA
                     LKIAPDLDDTQIEAIAELLRRHRVDGVIATNTTLDRSGVAGLMHADEAGGLSGRPLFE
                     KSTAVVSRLARALQGEVPIIAAGGILSGADARAKIAAGAELVQLYTGLIYRGPCLVTE
                     IGRALAAAEDPASPAKPHVQQG"
     gene            complement(11396..12148)
                     /locus_tag="VIS73_08145"
     CDS             complement(11396..12148)
                     /locus_tag="VIS73_08145"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEY5763164.1"
                     /translation="MARPDDSSLPFSLLQFYATTPYSCSYLPNRLARSQVATPTHLIN
                     AEIYGDLVRAGFRRSGVFTYRPHCDTCQSCVPVRVPVAEFRANRSQRRTWSRLTQGPQ
                     ALAARELPLALHRDHFDLYQRYQTARHAGGGMDRDDRDQYAHFLLQSRVDTRLIEFRE
                     HHVLRMVSIVDVLSDGLSSVYTFFDPDLPAASLGTYSILWQIAQCRALALPYVYLGYW
                     IRESRKMAYKVHFRPLEGRIDERWQTVSPPRP"
     gene            complement(12159..12860)
                     /gene="aat"
                     /locus_tag="VIS73_08150"
     CDS             complement(12159..12860)
                     /gene="aat"
                     /locus_tag="VIS73_08150"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY5763165.1"
                     /translation="MIPWLEADDPFPPVSRALKEPNGLLAAGADLSPERLIAAYRRGI
                     FPWFNDGDPPLWWSPDPRTILEPGAFHVSRSLLKCLRQQRFAVSSNRDFAAILNACAT
                     TPRAGQSGTWITSSMQAAYRTLHELGYAHSVEVWRDGKLAGGIYGVAMGRIFFAESMF
                     SRARDASKVALARLADALHGAGFDLIDCQLPTAHLHSLGARDIPRAEFAARVSAAIAT
                     DDPGEWPAASIATAD"
     gene            complement(12857..13960)
                     /locus_tag="VIS73_08155"
     CDS             complement(12857..13960)
                     /locus_tag="VIS73_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5763166.1"
                     /translation="MADLSVTSLVQRYGTHTVVDGVSFEVPPGRIACLLGPSGCGKTT
                     LLRCIAGFEEIAEGEIRIAGESCSRPGYRKAPESRRIGMVFQDYALFPHLTIEANVAF
                     GLHGLSSGDRGRRVETMLDLVGLQGQGRKYPNELSGGQQQRVALARALAPKPALVLLD
                     EPFSNLDVDLRERLSHEVRDILKACGSTAILVTHDQREAFAMADEIGVIAEGKLQQWD
                     PPYKLYHEPTNRFVADFIGEGAFLPGTVLDEHRIEVELGVLDSRAPIECERRACCTCG
                     LSCRLDVLLRPDDIIHDDASPVAAEVLHKAFRGAEILYTLRLPSGASVLSLVPSHHNH
                     ALGERIGIRLDVDHVVTFRQQNAEGRQAVAFHR"
     gene            14092..15780
                     /locus_tag="VIS73_08160"
     CDS             14092..15780
                     /locus_tag="VIS73_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY5763167.1"
                     /translation="MHALRANRLLLVALLLAFAVAMPVASVLANVFSGGTSDTWRHLA
                     ATVLPEYVANTLLLCFGVGVGTVIVGTASAWLTVTHDFPGRRLFSWALVLPMAMPAYV
                     MAYVYTDLLQYVGPLQTSLREWFGWGRRDYWFPDVRSAGGAVLMFVCVLYPYVYLLAR
                     TAFLERASGMIEAARMLGVGPWASFFRVSLPLARPAIVAGASLALMETLADYGTVSYF
                     AVPTFTTGIYRAWFSLGDRIAAAQLAAALLGFVILLLALERLSRGRAQFHDTTGRNRP
                     LAGRQLRGWKALAATLVCALPLFLGFVLPALLLGRLALSEEATLSLSRFAQLAGNSVL
                     LAGVTALFAVALAVLLGYSGRLSGGWPARVLNRLVSLGYAVPGSVIAVGVLIPVTRLD
                     NWLAGQWMEWFGVNPGLLLTGGIAALVYAYLVRLLSVALQTVQAGLTKITRSMDDAAR
                     SLGASQGRVILGIHVPLLRGTLFTAMLLVFVDVMKELPATLVMRPFNLDTLATQTYTF
                     AADERLAEAALPALAIVAVGLLPLMILSRQIARQRAGGGKQDGDPAGSPVTATC"
     gene            complement(15785..16786)
                     /locus_tag="VIS73_08165"
     CDS             complement(15785..16786)
                     /locus_tag="VIS73_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5763168.1"
                     /translation="MLAFLGACVLTPATQAAEATLNLYSARHYQTDEALYADFTRSTG
                     IKINRIEGKDEELLERLKNEGANSPADVLLLVDAARLAKADALDLFAPVPSKLLDARI
                     PKQLRTPTWSAFSTRARVIVYNKSEVKPEQVANYEDLANPALKGKVCTRSGSHPYNLS
                     LVASLVAHKGEAWVETWAKGVVANFARAPKGGDTDQIRAVAAGECGVAISNSYYLARL
                     MRSKKEDDKKVVEAIGVVWPNQAGNGTHINVSGAGVLKNAPNRDAAVRFLEYLASDSA
                     QVYFADGNNEWPVVAGVKTANPALEKLGSFKADDLPITEIAARQATAQKVLDRAGYR"
     gene            17111..18127
                     /locus_tag="VIS73_08170"
     CDS             17111..18127
                     /locus_tag="VIS73_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY5763169.1"
                     /translation="MNSKLAIPASFKAFVIRDKDRAVSAAFERWSLDQLDAGEVVLRV
                     SHSSVNYKDALAATGKGRIIRRFPCIGGIDMAGTVVSSADSRFTPGDAVLATSFDIGV
                     AHHGGYAEYARLPASWVIPLPAGLDAGSAMALGTAGFTAALAITRMEQNGLRPGNGPV
                     LVTGATGGVGSLATVMLAQLGYRVTALTGKAAERPYLESLGASEILLRQELDLERIKP
                     LDAARWAGAIDNLGGPVLAWAASTMRQAGTIASVGLAASHELHTTVMPFILRGASLLG
                     VDSGYIGEPLRSGLWRRLAGDLKPRGLASMTRTIGFDSLPAIFGEFIDGQVRGRTVVR
                     IAAD"
     gene            18144..19274
                     /locus_tag="VIS73_08175"
     CDS             18144..19274
                     /locus_tag="VIS73_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5763170.1"
                     /translation="MSAENELPKVLIVDDSRIVRATLSKHLKGQYSIREEGDGEAGWQ
                     ALTVDTEIQVVLSDLTMPKLDGYGFLERIRSSKVSRIRNIPVLLISGDEDESARQRAK
                     TLGASDFIAKGIGTTELLTRLKSLMQLAQTQTELDESRDQQVKDPVSGLFTRVYAVEQ
                     AAQMLAYASRHQVPSSIIVVAVDQARMIREKAGDAAANQLMKALGQIMLQKVRREDTL
                     AIYEPNAVAVVSPSTPDGGAMIFVERLRETFEQANITFQGKRLVLTASVGFASVPPDK
                     AADAIMLMELAGKRMTEAVAAGGNRVIGSAGHVAGPPGVPSLETALTLARAGRQAELT
                     PHLAPLVLRVLPLLAAANSPLGLGLDIDQLQQKLKGTARKDA"
     gene            19323..21215
                     /locus_tag="VIS73_08180"
     CDS             19323..21215
                     /locus_tag="VIS73_08180"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HEY5763171.1"
                     /translation="MSKVFDFHKRSIEDRDGFWTEQAQLIDWNKPFEKVYDYSKPPFV
                     KWFVGGKTNLCHNAVDRHAAKRPNDRALIYISTETNEEVVYTFAELQAEVMRMAAIYK
                     SLGVKKGDRILIYMPMIAQACFAILASARIGAIHSVVFGGFASGSLATRIDDAKPTLI
                     VSSDAGMRGGKAVPYKHLLDEAINLSAHKPGKVLMVDRGIDKGFNKVAGRDVDYATLR
                     AEHMNADVPCEWLESSEPSYILYTSGTTGKPKGVQRDTGGYAVALASSMKHIYCGNAG
                     ETYFSTSDIGWVVGHSYIIYGPLIAGMATIMYEGTPLRPDAGIWWKIVQDYKVNVMFS
                     APTAARVLKKQDPAFMHKYDLSSLRHIFMAGEPLDETTHEWFMTELGKPVIDNYWQTE
                     TGWPMLAAMPGIEQTKIKLGSPSFPVYGYDLRIFREDGSECGANEKGIAAVVPPLPPG
                     CLSTVWGQDERFVSTYFSLFKEPLVYSSFDWAIKDDNGYYTILGRTDDVINVAGHRLG
                     TREIEEAVQAHKAIAEVAVVGVEDKLKGQEPVAFAVVRDASAVATKEMRDELEKAIKK
                     IVDANLGAIARPKHIYFVTALPKTRSGKLLRRSLQALAEGRDPGDLTTIEDPTALEQI
                     KKALAG"
     gene            21415..21618
                     /locus_tag="VIS73_08185"
     CDS             21415..21618
                     /locus_tag="VIS73_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763172.1"
                     /translation="MQHSPITGQRLIAVFLAGSLLFNYPLTALVDAADTWLGVPATVA
                     YLFLVWAALIALMAWIIERPGDH"
     gene            21680..24442
                     /locus_tag="VIS73_08190"
     CDS             21680..24442
                     /locus_tag="VIS73_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY5763173.1"
                     /translation="MLSTGSIVAVSAAYLAVLFGVAYYADRRADQGRSVIASSTVYAL
                     SLAVYCTAWTYYGSVGRAASSGTGFLPIYIGPTLLVALWWFLMRKMIRISKAQRITSI
                     ADFISSRYGKSHLLGGLVTVIAVIGIVPYIALQLKAVSNSFTILSHYPEIVMPGGVSV
                     SSWFADTAFYVALILAAFTILFGTRHLDATERHEGLVAAIAVESVVKLVAYALVGAFV
                     TFFLYDGFGDIFARAAADPELARLLEFGAGGNSHATWFSLTVLSMLAFMFLPRQFQVA
                     VVENVNENHLRRAIWLLPLYLLLINIFVLPIALGGLLHFGGQGVDADTYVLTLPMAHH
                     AELLALVVFVGGLSAATGMVIVETIALSTMVCNDLVMPALLRWRGNWLQTHANLTGLL
                     LGIRRAAILIIVLLGYAYFRMAGDAYALVAIGLISFSAVAQFAPVMIGGMYWRGATRD
                     GALAGLTAGFLVWAYTLLLPSFAKSGWIPRDFLEFGPWGITLLRPQQLLGLTGLDEIS
                     HCMFWSLFANIGCYVGVSLVRPTRDVQVAGQARMFVDALSNVPPEGHGFWRGQAEVSD
                     LLALLGRFLGPDRARIAFVDYALARGLAGIDALEADAGLVHYAETLLTGAIGSASARV
                     MVSSVVTEEPLGIDEVMHILDEASQVRAYSHELENKSRELEAATRDLREANERLKELD
                     RLKDDFMSSVTHELRTPLTSIRAFSEILHGDPKVELAERKRFLGIIVSETERLSRLVN
                     QVLDLAKIESGNADWIEETVDMREVVEQSVAATSQLFNDRGVTLAVDMPRAVPGLTAD
                     HDRLVQVVINLLSNAVKFAPAGKGRVEVRLADTAEGLRVDVRDNGPGVRREDRESIFE
                     KFRQGSGTLGKPIGTGLGLPISRQIIEHYGGRFWLEPGAGQGACFSFILPLKSDSTIT
                     SRRPNP"
     gene            24439..24813
                     /locus_tag="VIS73_08195"
     CDS             24439..24813
                     /locus_tag="VIS73_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5763174.1"
                     /translation="MSKKVLIADDEQNIVISVEFLLKREGFTVSVAGDGEEALAKVRA
                     DRPDLVVLDVMMPKKTGFEVCQEMRVDAALKHVRVLMLTAKGRDTEVAKGLALGADAY
                     MTKPFSTAELVAKVKELLARET"
     gene            24857..>26612
                     /locus_tag="VIS73_08200"
     CDS             24857..>26612
                     /locus_tag="VIS73_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEY5763175.1"
                     /translation="MKRYYKLIAMTLAVAVFLVGFAGVLGALLWADMQPAERAVVGPI
                     FEQRLGGVLLLIFLSIATAFLALRALHAAYVLEPRKLASEIDALVQDAAQRLTARGPG
                     ELKAVIASVNRLAERRQQHEEDVEEQIRVANRNAEEERNRFAALVAEINLGVVVCNRD
                     GRILLYNPKARENFSGGKDGVAPLGLGRSIYTVLDRQLIAHALEGVGEGRSSASRFIA
                     ASPGGRLLRVQIAPVTNEAASEATVGYLLMLEDITATFDSESKRDELLQSLTEGSRGP
                     LGNLRAAAETLHTYADMPAEDRERFFKVIRQEAERLGGRIESAAHEFAAVVKARWPLE
                     EMRAADLLAAARRRLGERLTIPVAIEEVAEDLWVRVDSFSLLQAITYLAARLVDEYSI
                     RDLRLRGLAEGGRVHLDLIWSGAAMSTETVMQWELDPMALAGEHSPLTVRDVIDRCGG
                     EIWLQRERVSHRAFFRFLLPATDATEVALSAREAMDSRPEFYDFDLFHWAADSDVLDD
                     SLLGDLSYTVFDTETTGLNPSQGDEIIQIGAMRIVNRRLLRNEFFEQFIDPEMPLPGR
                     STEITGITPEMLAGQPVIA"
BASE COUNT         4432 a         8333 c         9175 g         4672 t
ORIGIN      
        1 ggaaggtaaa gccgaccggc accagcagta cgccgacgac gaattcacga atggtgcggc
       61 cgcgcgaaac gcgcgcgatg aacatgccga caaagggcga ccacgcaacc caccaggccc
      121 agtagaacag ggtccacccg ccgatccaac cgctgggttc gtaggcgtag aggttgaagg
      181 tcatgtcgaa cagattcgag atatacatcc cggtgttctg gaccatcgtc tgcaggagaa
      241 aaacagtcgg tccggccacc aacacaaaga ccagcagaag gatggcgaga atcatgttca
      301 attcggatac gcgccggatt ccggagtcga gccccaaact gaccgacagc gttgcgaagc
      361 cggtgatcac cgcaatcagg atcacctgca ccgtgaggct ggtggggacg tcaaagagat
      421 gattcaagcc ggcgttgacc tgcaagacgc cgaagcccag cgaagtcgcg acaccgaaaa
      481 tggtgccaag gacggcaaat acgtcgacgg cgtggccgat cgggccgttg atgcgatctc
      541 cgatgatagg gtaaagggac gatcgaatgg tcagcggcag gccgtgccgg aaactgaagt
      601 acgccaatga aagcgcgaca acggcgtaga tggcccacgc gtgcacaccc cagtggaaga
      661 aggtgatgcg catcgcctgg cgcgcggctt cggcagtctg cgggtcaccg acaggcgggg
      721 tgacgtagtg catgatcggt tcggccacac caaagaacat caggccgatg cccatgccgg
      781 ccgaaaacaa catcgcaaac caggatgcgt agctgtagtc cggcgtgctg tggtccgggc
      841 cgagcttgat ttcgccaaac cgggagacgg caagggcgat cacaaatatc aggaagctgg
      901 cgacagcaag aacgtaaaac cagccagcag actgcattgt ccagtcgcgg accacgccga
      961 agcccttggc cgcagcctcg ggaaacgcga cgccaaaaat gacaaaggcc agcgtcagaa
     1021 tcgccgaact gaagaatacc ggggggttga tcaggatgcg ctgcttggca atcccgcgag
     1081 gtagatcact catcgaactc tcactttatg tgcgcgcgga acggaacagt ctaacaaccc
     1141 gtgtcaaggc ggcccccgca agccgagcga gtgtggcgct cggtcggccg ctgtgcgctg
     1201 ctttccgcgg agcttggcga ccaggttgcg aaagatccgg tggcacccga ccgggccgcg
     1261 gttgctcttg atgtagacga gggtgattcg cgagggaagt acgcgcggtg ctgtaaggcc
     1321 ggaatccttg gagccgttca ccttcgaacg tcaagtccgc aaagcatcag ccatttttcc
     1381 agccgcgccg gcaggcgttc agtagtagct gtccctgccg aagaagggcg ggcggcgacc
     1441 gcaaaggctt tccagcagag tcagttcgac gtcggtatgg ttgatgacgt gcgccggctt
     1501 cattgccgcc tgaacggtat cgccgatttc gtagggcttg tcgtggtaac tcgttcggta
     1561 tcggtgaacg gcgttcggcg tggccgtggg catgacttcg acctcgccgt agcacaggca
     1621 gaaataggtg ctggccgttt cggcctcgat gtagcagccg gtaccgcgca cgccgatcgt
     1681 cgcggtcggc gtggtgatac gtttttcgcc cttgccgaat accgatagca gcttgccggt
     1741 gatgacgcgc agcgcatcga cggcggcctc gccggcgaag ctgacgctgc ttttctcgcg
     1801 ctgcaggtag gcgtccttgc cgatgacgta gatgatttcg gcgccggcat cggtgtcgac
     1861 ggtatcgccg gggccaaccg gcattcccgg ttcggccggc cggccgttga tgcgcgccgc
     1921 gcccttgacg cgatggacgc cgggggcgac gggttcgagc ccggcggcga gcaggcgctg
     1981 aacggcaccg agcgcgccgc cgagtccgat caggcttgca ccggcgaagc gcagcagcgc
     2041 gcggcgttgg cgtttcatcg tgatgctcca ttcgtcatgc gacgggcgat catcgtacct
     2101 ccacgatctt gcgaccgccg gcgaggcggg ccgttcagct gcccttgcag cgaatgcatt
     2161 cgagataggc ctcgtgatcg agccgtcctt cgcggctggc ccgccagacg gcctcgccga
     2221 ggcagtcgag caacacgtgc agcgcctcgt gcggctcgcg cgtggcggtg agattgcggt
     2281 aggcggcggc gatgccgggg ggctggtcga tcgacagctg ttcggcgatc gccaggtgca
     2341 gcgagagatg caggaaggga ttgacctcgc cgccttcagg cgtgaactcg cgcgacaact
     2401 cctcgaccgg gccttcgagt aaggcctggt attcgggatg cgcggcgacg atgtcggcgg
     2461 cgagcgcctc gagaccttcg agaacggcgc gttcgcgctg ctttttccag gcttcgcgaa
     2521 aaaagctgcg cacctcgtcg cgattgggcg tgaacatgga tttctcagtc aggtctggtg
     2581 tggaagaggg cgagcattac ggcattttgc agcaggtgat tggcgcggcc gtagggtgcg
     2641 acctgaccgc tgccgtagac gccgatgaag ggggtaccgg gcaggccgtc gcggattgcc
     2701 agccagtcgc ggtcctcgcc gccgtagaag tacgggccgc ggccgatgca cgaggcgaag
     2761 aatccgaagt cggccgggcg ttcgctgcgc tcacacaacg cggcgatggt tgcgcgcatg
     2821 tcggcctcgg cggcgaccgg ctggcggatc gcccaggtgt agcgctggcc tgccggcagg
     2881 gtctcgccga gcgtgagcga gccgtcggga ttaagcgcgg cgatcggaat caagcgcggc
     2941 gggtgccggg gcccgcgtcc ttcgccggcg ggcagtgcgg cgagcaggtg cagcggcagt
     3001 ggcaggccgg tgcggtattc cgcgggcagc gcacggcgca gcgaatcggc ggcgcttcgg
     3061 ccgccgatgg tgaggacgac gttgtcgtgg gcagcgtcga tttcccagtc gccgccgagc
     3121 atgcggacgc cggtcgagac ggcaatgtgc gtttcggcgc cgccgaccga cacttcgacg
     3181 ataggcggcg ttgccggccg cccttggctc caggccgggg ttgcgtgcgt gctcagcgcg
     3241 tcgctgtaga tcgtaccgaa gcgcggcggc agggcagccc aggccggcgg cagcgtgctc
     3301 gcgccggcga tggccagatg cggtccctgg ccgtcatcgg cggcatcgag tcgcagcggc
     3361 gcgccaaagg ccattgctac cgcggccggt cggtcgaaca cccagctgct ttcattggcg
     3421 acaccggcga cgatgccgcc ggcaatctgc aggcagttgc cggcgcggct cgcggcgtgg
     3481 atggccgtcg ccgcctgccg cgtgaaatga ggcgtgagga agagcagcac gccctgcgcg
     3541 tgggtgattc cggcgcgctc gagtgcgctg gcgacagcgc ttgcggcgag ttgcggcgag
     3601 gcgtcgttgg cggcgaccaa tgcggtggcg acggtcatcg tggtgcctgt ttcgcggcgg
     3661 cggcgccggc ccgcgcggcg ctcttgtccg gccagcggca gaggtcggcg accgggcacc
     3721 gggcacacgc cggccggcgg gccgtgcagg tatagcggcc gtgcaggatc agccagtggt
     3781 gcgcgtggcg gcggtactcg gccggaactg ccttgagcag agcgtcctcg accgcgcgcg
     3841 gggtcttgcc gggcgccagg ccgcagcggt tggcgacgcg gaagaggtgg gtgtcgacgg
     3901 cgatcgtcgg ctcgccgaag atggtgttta ggacgacgtt ggcggtcttg cggccgacgc
     3961 cgggcagggc ttcgagcgcc gcgcgctcgc gcggtacggc gccgccgtac tggtcacgca
     4021 ggacggcgca ggtcgcggcg aggtggcgtg ccttgctgtg gtagaggccg atgctgcgga
     4081 tgtagtcggc gataccctct tcgccgagtt cggccatcgc cgccgccgtc ggtgcgtcgc
     4141 ggaacagcgc ctcggtggcc ttgttgacgg cgcggtcggt ggtctgtgcc gagaggatga
     4201 cggcgaccag cagctggaac ggcgagtcgt agcgcagctc ggtcgtcggc tcgggattgg
     4261 cttcctgcag tcgtctgaag aattcgaggc gctgcgcgcg gttcatgtcg gcggttcggc
     4321 agggaatggg cgaactgtac attgcttcgg cggctgtcgc ggcatccggg aacggtggcc
     4381 gcagaacacg ggtccactgc tgggtgtgac gaggaaacga cgatgcgcgg attgaagctc
     4441 ctgtgggtct cgctggcggc gctggccgtt gccggctgcg cggcgacgcg cctgaagtcg
     4501 acctgggtcg atccgaccga ccgcggcggg ccgctggcca aggtcgccgt gctggtcttt
     4561 cacgaggacg ataacctacg ccgctttgcc gaggatcagg cctcgcgcac gctgatgggg
     4621 agcgcgcgca tcgtgcccgg atacaggttg ttcgagcggc ccgatcccga tgtcgacaag
     4681 gtcaaggacc ggctgatcgc cgagggtttc gatggcgtgc tgatggcacg cacggtctcg
     4741 gtcgatacgc agcagcagta cattccgccg cgcacgatgg cggtgccgac cgggccggtg
     4801 ctcgtcgggc ccttcgtcgg cgcccatggc ttcatcggct actaccggca cgtctggggt
     4861 tacacctacc agacgctgcc cggttacacg accgagcaga cgacggtggt tgtcgaatcg
     4921 gtactgtatc ggctgcctgg cggcaacccg gtctggtcgg ccgtttccga gacgcgcaat
     4981 cccgattcgg cggccgaggt ggtgcacgat ctggtgacga tgatcggccg cgaactcgcc
     5041 gaccgcgacc tgatcaggcg ctgacggccg acgccggcgg ccgcgccgca cgtcgccgca
     5101 cggcacgcgc atcgatcacg ttcttggcgg cgatcaggca accgagcagg atgaagccgc
     5161 cgggtggcag gatcgccagc agcagtccgg gatagtcctc gggcaagagg cgcagcggct
     5221 gccacccctc gaccagcagg tcgatgccgc cgaacagcgt gccgctgccg agcagctcgc
     5281 gcagcgcgcc gagcagcgcc agcgtccaca gcaggccaac ccccatgaag atgccgtcga
     5341 aggcggcgat cagcggcgga ttcttggcgg cgaaggcttc gacgcgggcg agcacgatgc
     5401 agttggtgac gatcagcggg atgaagatgc cgagcacgag ataaaggccg tgcaggtagg
     5461 cgttgaacag caggtcgatg acggtgacca gcgccgcgat gatcaggatg aacaccggaa
     5521 tgcggatctc gtagggaatc aggttgcgaa ggaccgcgac ggcgatgttg gcgatcatca
     5581 tcacgatgat ggtcgccaac gccagcgtcg tgccgttgat cgcgttggtg ctgacggcga
     5641 gcagcgggca gaggccgagt gcctgcacca gtgccgtgtt ctgactccag aggccgctgc
     5701 cggcgacctt cttcaggttg tcgcgatcga tcatgggcgt ttctccgcgc cgtcgtcgaa
     5761 cagcgttgcc tgttcggctt cgacgaattg cagtgccttg gcgactgtct tgatcaccgc
     5821 gcgcggcgtg accgttgcgc cggcgtaggc gtcgaattcg ccgccgtcct tgcgcacgcg
     5881 ccaggcggcg agcggccgcg tcaacagcga ctggccgtcg aactgggtaa tccacgggcg
     5941 tgtcttgttg cgatcctttt tcggctcgat gtagtcgccg aggctggggg tttcccggtg
     6001 ttcgaggacg cgcacgccga gtatgcgaag cttgcggtcg acagcgagca gcaggcggat
     6061 gcggccggcg tagccgtccg gtgccgttgc ctcgagcacg actgccgagg ccttgccgcc
     6121 gcgcatcgct cggtagccga tggtcgccgc cgcattgccg agccgcgggt cgtcggcgat
     6181 gctgatcctc gccgccagcg ggtcgttgtc gtagaccgac gccggcagca cctcggagat
     6241 caggcgcatg cgttcggctg ccgctgtggc cagaatcgcc gggcgtgtcg cccggtaggc
     6301 gccggccagc agcgccgtga agacgacgac gaagaccagc aggatcgtcg ccgtgcgcag
     6361 cgccattgcc ggcgcggtca gttcgcgcag gccggtcatg gtgcctcgcc cttattcgtt
     6421 gcgccgaaga ccggcggttg cgtgtacatg tcgatcagcg gcacgcagag gttcatcagc
     6481 agaatcgcga aggcgacgcc atcggggtag ccgccccaga gacggatgat gaccgtcagc
     6541 agcccggcgc cggcggcgaa aaccagcttg ccgcgcggcg tcgttgcacc gctgaccggg
     6601 tcggtggcga tgaagaaggc cgcgagcatg gtgccgcccg acagcaggtg gaagagcggc
     6661 gtggcaaatg cttcgggccg ccaaagccag aacagtcccg agaccaacgc gacggtaccg
     6721 agataggcga acggtatctg ccaggtgatg atccgacgcg ccagcaggaa aacgccgccg
     6781 agcaggtagg ctagcgccac ccactcccag ccgcggccac cgaagtagcc gtggacagct
     6841 gtgtcggcac gcagctggtc gacgctggca gcgccctcgg ccaagcgcag gccggtcttc
     6901 aggtggtcga gcggtgtcgc ctggatgatg ccgtcaacgc gttcgccgcc gaggatccag
     6961 ccgatctgcg tcgccgtgtc gacgtcggct gccggccact gcgacatcag tgccggaaag
     7021 gaaacgatgc agaccgcgaa gccgaccatc gccggattga acgggttctg ccccagtccg
     7081 ccgtagaggt gcttggcgac gatgatggcg aacagggtgc cgacgatgac cagccaccag
     7141 ggcgccagcg gcgagagaca cagcgccagc agccatgcgg tgagcagggc gctgcaatcg
     7201 ccgagcgccg gcgcgatcgg caggccgcgc aggcgcagca tcaaggcttc ggcagcaagc
     7261 gcggtgacgc tggcgatgcc cagtgtcagc agcacgccgg caccaaacag ggcgacccag
     7321 gccgcgatcg ccggcagcag cgcgaccatg accgtggcca tgaccgtgcg cacgctcgac
     7381 gcctggcgca tgaaggggga agtcgacagc gtgacgctca tgacggggga gtctcttcct
     7441 tcggggcggt cgccggttcg ggggcggcag cggctgccgc ggccgcttcg cgttggctgc
     7501 gtgtgcgttc gagcgcggcg gcgatctcgg cctgtttcgc ggccgcttcg gggtcggctg
     7561 cttgtgccgc ggccttggcc ttctgggcgg cggcggcccg ggccagtcgc tcggcctttt
     7621 cctgcttgtc gcgttcttcg cggaactggc ggaattcgaa acgcgagcgt gcgccctcgg
     7681 cggcgagacg ctcgcgttct cgcgaccaga tctcgccctt ggcgaagcgg aagtactgga
     7741 ccagcggaat gttcgacggg cagacgtagc tgcagcagcc gcattcgatg cagtcgaaca
     7801 gcgcgtactc ctgcgccttg ccgaagttct tcgcgcgcga ccaccagtac atctcgaagg
     7861 gttgcagttc gtgcgggcag gcacgcgcgc attcgccgca gcgaatgcac ggcatttcgg
     7921 gcggcggcgg tggcaagagc tctggcgagg cggcgatcag gcagttggtt gccttgacca
     7981 ccggggcgct ggtgtcgggc aggcggaagc ccatcatcgg gccacccatc agcacgccat
     8041 cggaatcggc cttcggtttg gccagggcaa tcgcgtcggc aacggacatg ccgatcggca
     8101 gttcccagtt acccggctgg ccgaccgaac cggctagcgt tacgacccgc gacagcacgg
     8161 gctccccgtg ggcgagcgcg cgccagaccg tgtaggcggt ggcgacgttg aagcactgca
     8221 cgccgaagtc ggtggtgcgc ttgaccgtgc cggcgatcgc cttgccggtc agtacgcgga
     8281 tcagctgctt ggcgcctccg gccgggtaga gcgtcggcac ggcgcggatc tcgaagccgc
     8341 tctcgccgat ttgcctggcg gcttcctgca gcgcggcgat cgcttcgggc ttgttgtcct
     8401 cgaccccgat cagcacgcgc ttaggctgca tcaggtcgcg tagcagggcg gtgccgcgca
     8461 ggatgtcctc ggcgcgctcg cgcatcaggc ggtcgtcgca ggtgatgaag ggttcgcact
     8521 cggcgccgtt gatcaccagt tcctcgagtg gccggcccgg tgtcgcgtcg agctttgcgt
     8581 ggctgggaaa ggcggcgcca ccgaggccga cgacgccggc gtcctgcagg cgctggcgcg
     8641 catcgacggg cgtcagcgtc ttgaggtcga ccgcgaggcg ctcgccccag cgctcctcgc
     8701 cgtcgggaac gatggtcact gccattgcgc tcaggcccga gggatggggt tgcggcgcca
     8761 gttcgaccgc ctcgacgcgc cccgaggtcg gcgcgtgcac ggcggccgaa atcatgccgt
     8821 cggcggcgcc gatcatctgt cccttgagca ccttgtcgcc ggtcttgacc agcggctttg
     8881 gtgtgccgcc tatgctctgg tgcagcggca cgaccaggcg ctcgggcagc gctgcccgag
     8941 cgatcggcgt gtcgcagctg tcttccttgt gcgctggcgg cttgacgcca ccgcggaagc
     9001 tgaacagcga agcaaagggc aggcgcgcgc tcatcgcgtc aggccgcccg cttgatttcg
     9061 atcaccgggt acttccactt ccaggtctgg atcgattcct cgatgctctc catgcggatg
     9121 cactcgaccg ggcagggcgg cacgcacagc tcgcagccgg tgcactggtc ggcaacgatc
     9181 gtgtgcatct gcttggcggc gccgacgatc gcatcgaccg ggcaggcctg gatgcacagc
     9241 gtgcagccga tgcaggtggc ctcatcgatg atcgccacct gcttcggctt ctcgacgccg
     9301 tgctcctcgg agagcggctt gtattcgcgg ccgagcaggt cggcgagctt gcgcacgcct
     9361 tcgtcgccgc ccggcgggca ttggttgatg tcggcctcgc ccttggcgat cgcctcggcg
     9421 tagggcttgc agcccggata gctgcactgc ccgcactgcg tctgcggcag gatcgcgtcg
     9481 atcttctcga ccagcggatc gcccttgacc ttgaagcgga ccgaggcaaa gcccagcgcc
     9541 gcgccgaggc agacggcgcc gagcgccatg atcaggatcg cgctggtcat tcgccgctcc
     9601 gccgatgcag gcgcggcccg ttcattggaa tcggtccaga ccggcgaagc ccatgaaggc
     9661 gaggctcatc agcccggccg tgaccatcgc gatcgccgtg ccgcgaaacg gcgccggcac
     9721 gctggcgccc tcgatgcgtt cgcgcaggcc gccgaagagg atcagcacca gcgagaagcc
     9781 gatcgcgctg cccatgccga acagcagcga ttcgacgaaa ccgtagccgt gcgaaatatt
     9841 gagcagcggc acgccgagca ccgcgcagtt ggtggtgatc agcggcaggt aaatgccgag
     9901 cacctgctgc aagagcggcg aggtcttggc gatcaccatt tcggtgagct ggacgacggc
     9961 ggcgatggtg acgatgaagg acagcgtgcg aaggtattcg agctcgaagg gctgcagaag
    10021 atagtggtcg atgacgaagg acgcgccggt tgccacggtc agcacgaagg tcgtcgccgc
    10081 gcccatgccg taggccgtct cgagcttctt cgagacgccc atgaacgggc acaggccaag
    10141 catcttgacc agcaccacgt tattgacgag aacggcgccg aggagaatga agaagtattg
    10201 cgtcatgaaa tgccgcagga aagggggcga ttatcgattg cgcagggttt cgcttcaacc
    10261 ctgctggaca tgcggctttg ccggcgacgc tgggtcttcg gcggcagcga gcgcgcggcc
    10321 gatttcggtg accagacagg ggccgcgata aatcagaccc gtatagagtt gcaccagttc
    10381 ggcgccggca gcgatcttgg cgcgcgcatc ggcacccgag aggatgccgc cggcggcgat
    10441 gatcggcacc tcaccctgca gcgcgcgggc caaccggctg acgaccgccg tcgacttctc
    10501 gaacagcggc cggcccgaca gtccgccggc ttcgtcggcg tgcatgagtc cggcgacgcc
    10561 gctgcggtcg agcgtggtgt tggtggcaat aacgccatcg acgcgatgcc ggcgcagcaa
    10621 ttcggcgatc gcctcgatct gcgtgtcgtc gaggtcgggc gcaatcttca gcgccagcgg
    10681 tgtgtacttg ccgtgggtct cggtgagccg ctgctggtcg gccttcagcg cgctaagcag
    10741 cgcgtcgagc gcgtcgtcgc gctgcaggtc gcgcaggttc ttagtgttcg ggctggagat
    10801 gttgatggtg acgtagctgg cggcggcgaa gaccttctcg aggcagatcc ggtaatcgtc
    10861 gacggcgcgt tcgatcggtg tgtcgaagtt cttgccgatg ttgatgccga gaacgccgcc
    10921 ttgtgccgga aagcgcgcgg ccgccacgtt ggcgagcagg cgctcgacac cgtcgttgtt
    10981 gaagcccatg cgattgatga tcgcctcggc gccggccagg cggaacagcc ggggcttggc
    11041 gttgccgggc tgcggccgcg gcgttaccgt gccgatttcg acgaagccga agccgattgc
    11101 ggccagtccg tcgattgctg caccgttctt gtcgaggccg gcggcgaggc cgacgcggtt
    11161 ggggaagtcg atccccatga cgcgcactgg cgtctccggg agtggcggcg agaccagatt
    11221 gacgagcctg ctgaccggcg aacgggcgag cgtcgcgatg gtgaggttgt gggcgtcctc
    11281 ggcgtcaagg gcgaacagcg cggagcgcat catcgtatag agcatgagcg attttaccgc
    11341 aacgcagcat cgcgaggcgg ctcgcaatgg ggcttccgtt ggtggagtcg gcggttcagg
    11401 gtcgcggtgg cgaaacggtt tgccagcgtt cgtcgatccg cccctcgagc gggcggaagt
    11461 gcaccttgta ggccatcttg cggctctcgc ggatccagta gccaaggtag acgtagggca
    11521 gggcgagcgc ccggcactgg gcgatttgcc agaggatgct gtaggtgcca agcgaggcgg
    11581 ccggcagatc gggatcgaaa aaggtgtaca ccgaggacag gccatcggag agcacgtcga
    11641 cgatgctgac catgcgcagg acatggtgct cgcggaattc gatcagtcgc gtatcgacgc
    11701 gcgattgcag caggaagtgc gcgtactggt cgcggtcgtc gcggtccatg ccgccaccgg
    11761 cgtggcgcgc cgtctggtag cgctggtaga gatcgaagtg gtcgcggtgc aatgccagcg
    11821 gcagttcgcg tgcagccagc gcctgcggac cctgcgtcag tcgcgaccag gttcgccgct
    11881 ggctgcggtt ggcgcgaaac tcggcgaccg gcacgcgtac cggcacgcag gactgacagg
    11941 tgtcgcagtg cggccggtag gtgaacacgc cgctgcgacg aaagccggcg cgaacgaggt
    12001 ccccgtagat ctcggcgttg atcagatgcg tcggcgtcgc tacctgcgag cgcgccagcc
    12061 ggttcggcag atagctgcag ctgtagggtg tcgtcgcgta aaactgcagc agcgagaacg
    12121 gcagcgagga gtcgtcgggt cgggccatgg attgactatc agtcggcggt cgcgatcgat
    12181 gcggcgggcc attcgccggg atcgtcggtg gcgatggccg cgctgacgcg ggcggcgaac
    12241 tcggcgcgcg ggatgtcacg ggcgcccagc gaatggaggt gcgcagtcgg cagctggcag
    12301 tcgatcaggt cgaagccggc gccgtgcagc gcgtcggcga gccgcgccag cgccaccttc
    12361 gaggcgtcgc gtgcccgcga aaacatagac tcggcaaaga atattcgtcc catcgctaca
    12421 ccatagatgc cgccggccag tttgccgtcg cgccagactt cgaccgagtg ggcgtagccg
    12481 agttcgtgca gggtccgata cgcggcttgc atcgacgacg tgatccaggt tcccgactgc
    12541 ccggctcgcg gcgtggtcgc acaggcgttg aggatcgctg cgaagtcacg gttactgctg
    12601 acggcgaagc gctgctgacg caggcatttc agcagcgatc gcgatacgtg aaaggcgccc
    12661 ggttcgagaa tcgtccgcgg gtctgggctc caccagagcg gcgggtcgcc gtcgttgaac
    12721 cagggaaaga tgccgcggcg gtaggcagca atcagacgct caggagaaag gtcggcgccg
    12781 gcggcgagca ggccgttcgg ctccttcagc gcgcggctta ctggcggaaa cggatcgtcc
    12841 gcttccagcc agggaatcat cggtggaaag cgacggcctg gcgaccttcg gcgttttgtt
    12901 gccggaaggt aacgacgtgg tcgacgtcga ggcgaatgcc gatccgttca ccgagcgcgt
    12961 ggttgtggtg cgagggaacc agcgaaagga cactcgcccc cgagggcagg cgcagcgtgt
    13021 agagaatctc ggcgccgcga aatgccttgt gcaggacctc ggcggcgacc gggctggcat
    13081 cgtcgtggat gatgtcgtcg gggcgcagca gcacatcgag gcggcacgac aggccgcagg
    13141 tgcagcaggc gcgtcgttcg cactcgatcg gtgcccggct gtcgaggacg ccgagttcga
    13201 cttcgatgcg gtgttcatcg agaaccgtgc cgggcaggaa tgcgccttcg ccgatgaagt
    13261 cggcgacgaa gcgattggtc ggctcgtggt agagcttgta gggcggatcc cattgctgca
    13321 gcttgccctc ggcgatgacg ccgatctcgt cggccatcgc gaaggcttcg cgctggtcgt
    13381 gggtgacgag gatcgccgtg ctgccacagg ccttgagaat gtcgcgcact tcgtgcgaga
    13441 gccgctcgcg caggtcgacg tcgaggttgg agaagggttc gtcgagcagc accagcgccg
    13501 gtttcggtgc cagcgcgcgt gccagcgcaa cgcgttgctg ctgcccgccg gacaactcgt
    13561 tgggatactt gcggccctgt ccctgaaggc cgacgagatc aagcatcgtc tcgacgcggc
    13621 gcccgcgatc gccggaggac aggccgtgca gaccgaaggc gacgttcgcc tcgatcgtca
    13681 gatgcgggaa cagcgcgtag tcctggaaga ccatgccgat ccgacggctc tcgggcgcct
    13741 tgcggtatcc cgggcggctg cacgattcgc cggcgatgcg gatttcgcct tcggcgattt
    13801 cctcgaagcc ggcgatgcaa cggagcagcg tggtcttgcc gcaacccgag gggccgagca
    13861 ggcacgcgat ccggcctggc gggacttcga aggaaacgcc gtcgacgacg gtatgcgtgc
    13921 cgtagcgttg gaccagcgaa gtaacggaca gatcagccat gcgggccatt ataatgagcc
    13981 gttccccggt gcgccgcatc cggtgcgccg cgagccgatc cctgtttcgg ccgtcccagc
    14041 ggcgggccgg catgcgcccg gtctcttctg ccacgactca cgacgcgatc catgcacgcc
    14101 ctgcgcgcga accgactgct gcttgttgcc ctgctgctcg ccttcgcggt tgcgatgccg
    14161 gtagccagcg tgctcgccaa cgtgttctcg ggcggcactt cggatacctg gcggcacctg
    14221 gcggcgaccg tgcttcccga atacgtcgcc aatacgctgc tcctctgctt cggcgttggt
    14281 gtcggcacgg tcatcgtcgg tacggcgagc gcctggctga cggtgaccca cgatttccct
    14341 ggccggcgtc tgttttcctg ggcgctggtc ctgccgatgg cgatgccggc ctacgtcatg
    14401 gcctacgttt ataccgacct gctgcagtac gtcggaccgc tgcagaccag cctgcgcgag
    14461 tggttcggct ggggtcgacg cgactactgg ttccccgatg tccgcagcgc cggcggggct
    14521 gttctgatgt tcgtctgcgt gctttatccc tatgtatacc tcttggcgcg aaccgccttc
    14581 ctcgagcgcg ccagcggcat gatcgaggcc gcgcgcatgc tcggcgtcgg accgtgggcg
    14641 agcttttttc gcgtttcgct gccgctggcg cgacctgcga tcgtcgccgg cgccagcttg
    14701 gcgctgatgg aaacgctggc cgactacggc acggtttcct atttcgcggt gcccaccttc
    14761 acgaccggca tctaccgcgc ctggttctcg ctgggcgacc ggattgccgc agcacagctc
    14821 gccgcggccc tgcttggttt cgtcatcctc ctgctcgcgc tcgaacgcct ttcgcgcggc
    14881 cgcgcgcagt ttcacgacac caccgggcgc aaccggccgc ttgccgggcg gcaactgcgt
    14941 ggctggaagg cgctggcggc gacgctggtg tgcgccttgc cgctgtttct cggtttcgtg
    15001 ctgccggcct tgctgctcgg gcggctggcc ttgtccgaag aggcgacgct gtcgctgtcg
    15061 cgctttgcgc aactggccgg caacagcgtc ctgctcgccg gggtgacggc gctcttcgcc
    15121 gtggcgctgg ccgtgctgct cggttacagc ggtagactga gcggtggctg gccggcacgc
    15181 gtgctcaacc ggctggtgtc gctgggctac gcggttcctg gatcggtgat cgccgtcggc
    15241 gtgctgatac cggtgacgcg cctcgacaac tggctggccg ggcagtggat ggagtggttc
    15301 ggcgtcaatc ccggcttgct gctgaccggc ggaatcgcgg cgctcgttta tgcctatctg
    15361 gtccgtctgc tctcggtggc gctgcagacg gtgcaggccg gcctaaccaa gatcacccgc
    15421 agcatggacg atgcggccag gagtctcggt gcgtcgcagg gcagggtgat cctcggtatt
    15481 cacgtgccgc tgctgcgtgg caccctgttc acggcgatgc tgctggtctt tgtcgacgtg
    15541 atgaaggagt tgccagcaac gctggtgatg cggcccttca acctcgatac gctcgcgacg
    15601 cagacctata cctttgccgc cgacgagcgt ctcgccgaag cggccttgcc ggcgctcgcg
    15661 atcgttgccg ttgggttgct gcccttgatg atcctgtcgc ggcagattgc ccggcagcgc
    15721 gccggcggcg gtaaacagga cggggacccc gcagggtccc cggtgacggc gacgtgctga
    15781 tcggctagcg atagccggcg cggtcgagca ccttctgcgc agtggcctga cgggcggcga
    15841 tctcggtgat cggcagatcg tcggccttga aactgccgag tttctccagt gccgggttgg
    15901 cggtcttgac gccggcgacg accggccatt cgttgttgcc gtcggcaaag tagacctgcg
    15961 ccgaatcgct tgccagatac tcgaggaagc gtaccgccgc gtcgcggttc ggggcgttct
    16021 tcagcacgcc ggcgcccgag acgttaatgt gggtgccgtt cccggcctga ttcggccaga
    16081 ccacgccgat cgcctcgacg accttcttgt catcttcctt ttttgagcgc atcaggcgcg
    16141 ccaggtagta ggagttgctg atcgcgacgc cgcactcacc ggccgcgacg gcacggatct
    16201 ggtctgtatc gccgcccttc ggcgcgcgtg cgaagttagc gacgacgccc ttcgcccagg
    16261 tctcgaccca ggcctcaccc ttgtgcgcga ccagcgaggc caccagcgac aggttgtagg
    16321 ggtggctgcc cgaacgcgtg cagaccttgc ccttcagcgc cggattggcc aagtcctcgt
    16381 agttcgccac ctgttcgggc ttcacttcgc tcttgttgta gacgatgacg cgggcgcgcg
    16441 tcgaaaaggc tgaccaagtc ggcgtgcgca gctgcttggg aatccgggcg tcgagcagct
    16501 tcgacggaac cggcgcgaaa aggtctagtg cgtcagcctt ggcgaggcgc gcggcgtcga
    16561 ccagcagcag tacgtcggcg gggctgttgg cgccttcgtt cttgagtcgc tcgagcagct
    16621 cttcatcctt gccctcgatg cggttgatct tgattccggt gctgcgggtg aagtctgcgt
    16681 aaagggcttc gtcggtctgg taatggcggg ccgagtagag gttcagcgtt gcttcggcgg
    16741 cctgagttgc cggggtcagg acgcaggcgc cgaggaaggc cagcatcgta cgggaggcgc
    16801 ccaggcgttt gctgagggaa gggttcgggg actgcttgcg gcgagcattc atcgagtatc
    16861 tccaaagttg agggttgggt tggcagggga atcgcagcca tgccgcgaat ttgatgcggc
    16921 ggattatgcg tgcctgcttc tgttttgtaa ataagaatcg ttcgcaacaa aaattgtgac
    16981 gcgccaccat gtgccgccga ggattcgccg cgcccgccgc cgctgatgcc ggcggaggac
    17041 gcaagcggtg ggccggcagc gcggtcaggc ttgccggtcg ccgccggttt tgtgtttata
    17101 atccgtaatt atgaattcaa aactcgcgat cccggcgtcg ttcaaggcct tcgtcatccg
    17161 cgacaaagac cgcgccgtca gtgcggcctt cgaacggtgg tcgctcgacc aactcgacgc
    17221 cggcgaggtc gtgctgcggg tgtcgcattc gtcggtcaac tacaaggacg cgctggcggc
    17281 gaccgggaaa gggcgaatca tccggcgctt tccctgcatc ggcggcatcg atatggcggg
    17341 aaccgtggtc agcagtgcag actcgcgctt cacgccgggc gacgccgtgc tggcaacgag
    17401 tttcgacatc ggcgtcgcgc atcacggggg ttacgccgag tacgcgcgac tgccggcgag
    17461 ctgggtgatt ccactgccgg ccggccttga tgccggaagc gcaatggcgc tgggcaccgc
    17521 gggctttacg gcggcgctgg cgattacccg catggaacaa aacggattgc ggcccgggaa
    17581 tggtccggta ttggtcaccg gcgcaaccgg tggcgtcggt tcgctggcga ccgtgatgct
    17641 ggcgcagctt ggctatcgcg tgaccgcgct gaccggcaag gctgccgagc gcccctacct
    17701 cgagtcgctc ggcgcgagcg aaatcctgtt gcgccaggaa ctcgacctcg aacgaatcaa
    17761 gccgctcgac gcggcacgct gggcgggtgc catcgacaac ctgggcggtc ccgttctcgc
    17821 ctgggccgcc tcgaccatgc gccaggcagg gaccatcgcc agcgtcggtc tggccgccag
    17881 ccatgagctg cacaccacgg tgatgccctt tatcctgcgc ggcgcatcgc tgctcggcgt
    17941 cgattccggc tacatcggcg aaccgctgcg cagtgggctg tggaggcgac tggccggcga
    18001 cctcaagccg cgcggactcg catccatgac gcgcacgatc ggtttcgatt cgctgcccgc
    18061 gatcttcggc gagttcatcg acgggcaggt ccgcgggcga accgtcgtgc gcatcgccgc
    18121 cgattgacgt agcgtccgcc ccgatgagcg ccgagaacga acttcccaag gtcctgatcg
    18181 tcgacgattc gcgaatcgta cgcgcgacgc tttccaaaca cctcaagggt cagtattcga
    18241 ttcgtgagga aggcgacggt gaggccggct ggcaggccct gaccgtggat accgaaatcc
    18301 aggtcgtgct ttccgacctg acgatgccga agctcgacgg ctacggcttt ctcgagcgga
    18361 tccgcagctc aaaggtctcg cgcatacgca acattccggt cctgctgata tccggcgacg
    18421 aggacgaatc agcaaggcag cgggcgaaga cgctcggcgc ctcggacttt atcgccaagg
    18481 gcatcggcac caccgaactg ctgacgcgac tgaagtcgct gatgcagctg gcgcagacgc
    18541 agaccgaact cgacgaaagt cgcgatcaac aggtcaagga cccggtcagc gggctgttca
    18601 cgcgcgtcta cgccgtcgag caggcggcgc agatgctcgc ctacgcgagc cgccatcagg
    18661 tgccgagcag catcatcgtg gttgccgtcg atcaggcacg gatgatccgc gagaaggccg
    18721 gtgatgcggc agcgaaccaa ctgatgaagg cgctcggaca gatcatgctg cagaaggtca
    18781 ggcgcgagga cacgctggcg atctacgaac ccaacgcggt ggccgtcgtg tcgccgtcga
    18841 cccccgacgg tggggcaatg atctttgtcg aacgcctgcg cgagaccttc gagcaggcaa
    18901 acatcacctt tcagggtaaa cgtctagtac tgaccgccag cgtcggcttt gcctcggtgc
    18961 cgcccgacaa ggcagccgac gccatcatgc tgatggaact cgccggcaag cgaatgaccg
    19021 aggcggttgc cgccggtggc aaccgcgtga ttggcagtgc cggccatgtg gcagggccgc
    19081 cgggagttcc aagcctggag acggcgctga cgctggcgcg ggccggacgg caggcggaac
    19141 tgacaccgca ccttgcgccg ttggtgctgc gtgtattgcc cttgctagcc gcggcaaata
    19201 gcccgctcgg tctgggcctc gatatcgatc aattgcagca aaaactcaag ggaacggcgc
    19261 gcaaagacgc ctgatcctcc ccgttaggca gtgcaggaag ttaatcaaga gaggaaatgt
    19321 tcatgagcaa ggtattcgat ttccacaagc gttccatcga agacagggat ggtttctgga
    19381 ccgagcaggc ccagttgatc gattggaaca agcccttcga aaaggtctac gactattcca
    19441 agccaccctt cgtcaaatgg tttgtcggcg gcaagaccaa cctctgccac aacgccgtcg
    19501 accggcacgc cgccaagcgt ccgaacgacc gcgcgctgat ctacatctcg accgaaacca
    19561 acgaagaggt cgtctatacc ttcgccgagt tgcaggccga agtgatgcgc atggcggcga
    19621 tctacaagag cctcggcgtc aagaaaggcg atcgcatcct gatctacatg ccgatgattg
    19681 cgcaggcctg cttcgccatc ctcgcctcgg cccggattgg cgcaatccat tcggtggtct
    19741 tcggtggctt tgcttccggt tcgctcgcga cccgcatcga cgacgccaag ccgacgctga
    19801 tcgtttcctc ggatgcgggc atgcgcggcg gcaaggccgt tccgtacaag cacctgctcg
    19861 acgaggcaat caatctgtcg gcacacaagc cgggcaaggt cctgatggtc gaccgcggaa
    19921 ttgacaaggg tttcaacaag gtcgccggtc gcgacgtcga ctacgcgacg ctgcgcgccg
    19981 agcacatgaa tgccgatgtg ccctgcgagt ggctggagtc gtccgagccc tcgtacatcc
    20041 tgtacacctc gggtaccacc ggcaagccga agggcgtgca gcgcgacacc ggcggctacg
    20101 ccgtggcgct cgcctcgtcg atgaagcaca tctactgcgg caatgccgga gagacctact
    20161 tctcgacttc cgacatcggc tgggtcgtcg gccattccta catcatctac ggaccgctga
    20221 tcgccggcat ggccacgatc atgtacgaag gaacgccgct ccgtcccgac gccggcatct
    20281 ggtggaagat cgttcaggac tacaaggtca acgtgatgtt ctcggcgccg accgcggcgc
    20341 gcgtgctgaa gaagcaggat cccgctttca tgcacaagta cgacctgtcg tcgctgcgcc
    20401 acatcttcat ggccggcgaa ccgctcgacg agaccaccca cgaatggttc atgaccgaac
    20461 tgggcaagcc ggtcatcgac aactactggc agaccgaaac cggatggccg atgctggcgg
    20521 cgatgcccgg tatcgaacag accaagatca agctgggttc gccgagcttc ccggtctacg
    20581 gctacgacct gcgcatcttc cgcgaggacg ggtcggaatg cggtgccaac gagaagggaa
    20641 tcgcggccgt cgttccgccg cttccgccgg gctgcctgtc gacggtctgg ggccaggacg
    20701 agcgcttcgt ctcgacctat ttctcgctgt tcaaggagcc gctggtctac tcatccttcg
    20761 actgggcgat caaggacgac aacggttact acaccatcct tggccgcacc gacgacgtga
    20821 tcaacgttgc cgggcaccgt ctcggcacgc gcgaaatcga agaggcggtc caggcgcaca
    20881 aggcgatcgc cgaagtcgcc gtcgttggtg ttgaggacaa gctcaagggt caggaacccg
    20941 ttgcctttgc cgtcgtgcgt gatgcgtctg cggtggcgac caaggaaatg cgcgacgagc
    21001 tggagaaggc aatcaagaag atcgtcgatg cgaatctcgg ggcgatcgcc cggccgaagc
    21061 acatctactt cgtcacggca ctgccgaaaa cgcgctccgg caaactgctt cgccgttcgc
    21121 tgcaggcatt ggcggaaggg cgcgatcccg gcgacctgac aacgatcgaa gacccgacgg
    21181 cactcgagca gatcaagaag gccctggccg gctgatcggc ggtagctttg ctgagaccgg
    21241 cgggtcggcg tgtgccggcc cgccggcgtt tccaccgcag ctcctgagtg ggcggtggta
    21301 agtagtgaac ggttttcgcg catccgccgg ccgggcgcgg tgcctaatcg tctgcagcaa
    21361 acatggccag tggtgctttg tcgcgcccgg tagagctgtg acgaggagag gtggatgcag
    21421 cattcaccga taactggaca acgtctgatc gcggtgttcc tcgccggatc gctgctgttc
    21481 aactatccgc tgaccgcgtt ggtcgacgcc gccgacacct ggctcggcgt cccggcgacg
    21541 gtcgcctatc tctttcttgt ctgggccgcg ctgatcgccc tgatggcatg gatcatcgag
    21601 cggcccggag atcactgatg cgctcagtga ccaggcgcgc tgatacgcgc tgcgccagcg
    21661 ggtcgaaggc gatcgctccg tgctgagtac cggcagcatc gtcgcggtct cggcggccta
    21721 tctagccgtg ctgttcgggg tcgcttacta cgccgatcga cgtgccgatc agggacgttc
    21781 ggtgattgcc tcgtcgaccg tctatgcgct gtcgctggcg gtctactgca cggcctggac
    21841 ctactacggc agtgtcggac gcgccgcgtc ctcgggtacc ggcttcctgc cgatctacat
    21901 cggcccgacg ctgctcgtgg cgctgtggtg gttcctgatg cgcaagatga ttcgcatcag
    21961 caaggcgcag cggatcacct cgattgccga tttcatttcc tcgcgctacg gcaagagcca
    22021 tctgctcggc ggactggtca cggtcatcgc ggtgatcggc atcgttccct acatcgccct
    22081 gcagctcaag gcggtgtcga acagcttcac cattctttcc cactatccgg aaatcgtcat
    22141 gccgggcggc gtttcggtgt cgtcgtggtt tgccgatacg gccttctacg tcgcgctgat
    22201 tctcgccgcc ttcaccatcc ttttcggcac gcgacacctc gacgcgaccg aacggcacga
    22261 gggcctggtt gcggcaatcg ccgtcgagtc ggtggtcaaa ctcgtcgcct acgcgctggt
    22321 cggcgccttc gtcacctttt tcctctacga tggtttcggc gacattttcg cgcgcgccgc
    22381 cgccgatcct gaactggccc ggctcctcga gttcggcgcc ggcggcaata gccatgcgac
    22441 ctggttttcg ctgaccgtgc tgtcgatgct ggcgttcatg tttctgccgc ggcagttcca
    22501 ggtcgccgtg gtcgagaacg tcaacgagaa ccatctgcgc cgcgcgatct ggctgctgcc
    22561 gctctacctg ctgctgatca atatcttcgt gctaccgatc gcgctcggcg ggctgctgca
    22621 tttcggcggc cagggcgtcg atgccgatac ttacgtgctg accctgccga tggcgcacca
    22681 cgccgaactg ctggcgctgg tggtctttgt cggcggcctg tcggcagcga ccggcatggt
    22741 catcgttgag acgatcgcgc tgtcgacgat ggtttgcaac gatctggtaa tgccagcgct
    22801 gcttcgctgg cgtggcaact ggctgcagac gcacgccaac ctgaccggcc tgctgctcgg
    22861 cattcgccgc gcggcgattc tgatcatcgt gctgctcggc tatgcctatt tccgtatggc
    22921 aggcgatgcc tatgccctgg tcgcgatcgg gctgattagc ttttcggccg tggcgcagtt
    22981 cgcgccggtg atgatcggcg gcatgtactg gcgcggggcg acgcgcgatg gggcgctggc
    23041 cggattgacg gcgggctttc tggtctgggc ctataccctg ctgctgccgt cctttgccaa
    23101 gtcgggctgg ataccacgcg actttctcga gttcggcccc tggggcatca ccctgctcag
    23161 gccgcagcag ctgctcggcc tgaccggact cgacgagatt tcgcactgca tgttctggag
    23221 tctcttcgcc aatatcggct gttacgtcgg tgtgtcgctg gtgcggccga cgcgcgacgt
    23281 gcaggtggcc ggacaggcgc ggatgttcgt tgatgcgctg agcaacgtgc cacccgaagg
    23341 ccacggattc tggcgggggc aggccgaggt cagcgacctg ctcgcgctgc tcggacgttt
    23401 tctcggaccg gatagggcgc gcatcgcctt cgtcgactat gcgctcgctc gcggcctcgc
    23461 gggaatcgat gcgctcgagg ccgatgccgg tctggtgcac tacgcggaga cgttgctgac
    23521 cggcgcgatc ggcagtgcgt cggcgcgggt catggtgtcg tcggtggtca ccgaggagcc
    23581 gctgggaatc gacgaggtaa tgcacatcct cgacgaggcc tcgcaggtcc gggcctatag
    23641 ccacgaactc gagaacaagt cgcgagagct tgaagcagca acccgcgacc tgcgcgaagc
    23701 gaacgagcgc ctgaaggagc tcgatcgact caaggatgac ttcatgtcat cggtcaccca
    23761 tgagttgcgg acgccgctca cgtcgatccg cgccttctcg gaaatcctgc acggcgaccc
    23821 gaaggtcgag ctggccgagc gcaagcgttt cctcggcatc atcgtcagcg agaccgagcg
    23881 cttgagccgt ctcgtcaatc aggtccttga cctagccaag atcgagtcgg gcaacgccga
    23941 ctggatcgag gagaccgtcg acatgcgcga agtcgtcgag cagtcggtcg ccgcgaccag
    24001 ccagctgttc aacgatcgcg gcgtcacgct cgccgtcgac atgccgcgtg ccgtgcccgg
    24061 tctgacggcc gatcatgatc gcctggtcca ggttgtcatc aacctgcttt ccaatgcagt
    24121 caagttcgcg ccggcaggca aggggcgcgt cgaggttcgg cttgccgaca ccgcagaggg
    24181 gttgcgcgtc gatgttcgcg acaacggccc cggcgttcgt cgcgaagacc gcgaatcgat
    24241 cttcgagaag ttccggcagg gcagcggtac gctgggcaag ccgatcggta ccgggctcgg
    24301 gctgccgatc agccgccaga tcatcgagca ctacggcggt cgattctggc tcgaacccgg
    24361 cgcaggacag ggcgcgtgtt tctcgtttat cctgccccta aagagtgatt caacaataac
    24421 gagcaggaga cccaacccat gagcaagaag gtgctgatcg ccgatgacga gcagaacatc
    24481 gtcatttcgg tcgagtttct tctcaagcgc gaaggattta cggtgagcgt cgccggcgac
    24541 ggcgaggagg cgctggcgaa ggttcgtgcc gaccggccgg atctcgtcgt gctcgatgtg
    24601 atgatgccga agaagaccgg attcgaggtc tgtcaggaaa tgcgcgtcga tgctgcgctc
    24661 aagcatgtcc gcgtgctgat gctgacggcc aaggggcgcg ataccgaagt cgccaagggg
    24721 ctggcgctcg gcgctgatgc ctacatgacc aagccgttct cgaccgcgga actggtcgcc
    24781 aaggtcaagg aactgctggc acgcgaaaca tgagcgtggt cgttcggact gcaggcatcg
    24841 acggagcgcc caagccgtga agcgctacta caagctgatc gcgatgacgc tcgcggtcgc
    24901 cgtgtttctg gtcggcttcg ccggggttct cggcgcactt ctctgggccg acatgcagcc
    24961 agccgagcga gccgtcgtcg ggccgatatt cgagcagcgc ctgggtggcg tgttgctgct
    25021 gatcttcctg tcgattgcga cggcctttct cgccctgcgc gcgttgcatg cggcctacgt
    25081 gctcgagccg cgcaagctcg cctcggagat tgacgcgctg gtccaggacg cggcgcagcg
    25141 actgactgcg cgcggaccgg gcgagctgaa ggcagtcatc gcctcggtaa atcggctggc
    25201 cgagcgacgg cagcaacacg aagaggacgt cgaagagcag attcgggtgg ccaatcgcaa
    25261 tgccgaggaa gaacgcaacc gattcgctgc actggtcgcc gagatcaacc tcggcgtcgt
    25321 cgtctgcaat cgtgacggcc gaatcctgct ctacaacccg aaggcccgcg agaatttcag
    25381 cggcggcaaa gacggcgtcg caccgctggg gctcgggcgc tcgatctata cggtgctcga
    25441 ccggcaactg atcgcccacg cactcgaagg cgtcggcgaa ggccgcagca gcgcatcgcg
    25501 tttcattgcc gcctcccccg gcgggcgcct gctgcgcgtg cagatcgcgc cggtgaccaa
    25561 cgaagccgcg agcgaagcga cggtcggcta cctgctgatg ctcgaggaca tcaccgcgac
    25621 cttcgacagc gaatcgaagc gcgacgagct gctccagtcg ctgaccgaag gcagtcgtgg
    25681 accgctaggc aacctgcggg cggcggccga gacgctgcat acctatgccg acatgccggc
    25741 cgaggaccgc gagcgattct tcaaagtcat ccgccaggag gccgagcgcc tcggcggtcg
    25801 aatcgaatcg gcggcccatg agttcgcggc ggtggtcaag gcgcgctggc cgcttgagga
    25861 aatgcgcgct gccgacctgc tcgccgccgc ccgccgacgg cttggcgaaa ggctgacgat
    25921 tccggtggcg atcgaagagg tcgccgagga cctctgggtg cgcgtcgaca gtttttcgtt
    25981 gctgcaggcg atcacctatc tcgcggcgcg gctggtcgac gagtactcga ttcgcgacct
    26041 ccgcctccgc ggcctagccg agggcgggcg cgtccacctc gacctgatct ggtcgggtgc
    26101 ggcgatgagc accgagaccg tcatgcagtg ggagctcgac ccgatggcgc tggccggcga
    26161 gcactcgccg ctgactgtgc gcgacgtgat tgatcgctgc ggcggcgaga tctggctgca
    26221 gcgcgaacgc gtcagtcacc gtgccttctt ccgtttcctg ctgccggcga ccgacgctac
    26281 cgaggtcgcc ctcagcgccc gcgaggcgat ggacagccgg cccgagttct acgatttcga
    26341 ccttttccac tgggccgccg acagcgacgt gctcgacgac tccctgctcg gcgacctcag
    26401 ttacacggtc ttcgataccg agacgacggg cctgaatcct tcgcagggtg acgagatcat
    26461 tcagatcggc gcaatgcgca tcgtcaatcg gcgccttctc aggaacgagt ttttcgagca
    26521 attcatcgat cccgagatgc cgctgcccgg gcgctcgacc gagatcaccg gcatcacgcc
    26581 ggagatgctc gcgggccagc cggtgattgc ca
//
LOCUS       DATDAA010000257         5616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_193556,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000257 DATDAA010000000
VERSION     DATDAA010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193556"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1843
                     /gene="soxB"
                     /locus_tag="VIS73_08205"
     CDS             122..1843
                     /gene="soxB"
                     /locus_tag="VIS73_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HEY5763176.1"
                     /translation="MSMNRREFVQLLAVATAGGLPLSRALAGDNAAGALYDLPRFGQV
                     HLLHFTDCHAQLMPIYFREPSVNIGVADARGAPPHLVGEHLLRHFGIKSGSREAHAFT
                     YLDFAEAANRFGKVGGFAHLATLVRRLRATRPGALLLDGGDTWQGSGLSLWTNGQDMV
                     DACKLLGVDVMTGHWEFTLGADRVKEIIDKDFKGTLDFVGHNVKTLDFGDPVFQPYTL
                     REMNGVSIAVIGQCFPYTPIANPRWQVPDWTFGIQEEALQPVIDEARGKGAQVVVLLS
                     HNGMDVDLKLAGRVTGLDAILGGHTHDGIPAPISVKNAGGQTLVTNAGSNGKFLGVLD
                     FEVKNGRIADFRYKLLPVFSNLLPADAEMAAYIERVRAPYAAKLAEPLAVSEELLYRR
                     SNFNGTFDQLILDALMEVKGAEIGFSPGFRWGTTVLPGQTITREHLMDQTAITYPWST
                     LTDMSGEMIKTILEDVCDNLFNPDPYYQQGGDMVRVGGMSYTCAPNEKIGNRIQDMRL
                     NGKPIDAKKTYKVAGWAPVAEAARDAGGQPIWEVVETWLRAKKTIAPRKLNVPRLSGV
                     AGNLGIA"
     gene            1906..2424
                     /locus_tag="VIS73_08210"
     CDS             1906..2424
                     /locus_tag="VIS73_08210"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY5763177.1"
                     /translation="MGRLDSTVVAGSLIAATLLASGLATAAEKLTTQAATLTTGAALK
                     VAQTALADCQKGGYVVAVAVVDRSGIPLAVLRDNLAGPHTPETAIRKAWTAVSFRNNT
                     SSLLDLSGPGKPSSGIRDIPGAIVLGGGLTINAKGLLLGGVGVSGAPGGHLDDACGNA
                     GIKAIQDALELE"
     gene            complement(2484..3695)
                     /locus_tag="VIS73_08215"
     CDS             complement(2484..3695)
                     /locus_tag="VIS73_08215"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEY5763178.1"
                     /translation="MKLKRIAALTALALVPGISSATVGYFAHGYGMKSIGMGGVGIAL
                     PQDGLAAASNPAGMAWVGDRYDIGLNLFRPDRDATLGSGFGPAAGTFDGNGDENFFIP
                     EFGYNRTFDSTMSLGLSIYGNGGMNTSYENGVGNGFVPQFGANKPVGVDLAQLFIAPT
                     VSFKINSAHSVGASINFAYQRFKAEGLQNFGINNPGYDDSTGWGVRLGWLGELAPGVK
                     LGATWQSKTDMSKFDKYAGLFAEQGDFDIPENYGLGISFDAGSGLMIAADVMEIKYSD
                     VKSIANTGNAATVLGAADGPGFGWQDMTVYKVGVSYALNGNTVLRAGYATGKQPIPSN
                     ETFFNILAPGVVEDHLTLGATFKLEDRSEITLGYMHAFENTVRGTGSTAGIDLRMAQD
                     SFGMAYARQFK"
     gene            complement(3917..5038)
                     /locus_tag="VIS73_08220"
     CDS             complement(3917..5038)
                     /locus_tag="VIS73_08220"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEY5763179.1"
                     /translation="MEFTIHNQVLLLVFLIALVMGAVANKTNFCTMGAVSDWVNMGDT
                     GRLRAWLLAMALALTGVLVLEGSGTVSLDSEVFPPYRTANFAWLRYLLGGFMFGIGMT
                     LAGGCGNKTLVRIGGGNLKSLVVLAVASFFAYQMLWGDLYGAAFDSWISATTINLGAS
                     GMSSQTVPAILGGWFGIEGKGFHYGLGALIALGMLAFIFSQHEFRKSFDNILGGSVIG
                     LAVVAGWWISGGRMGAAWKEWADFATEAPSRVMVQSYTFISPMGDSARYLMEPTKLSF
                     INFGIMALSGVILGSLVYALFKKQFRIEWFHGREDVINHLVGGVLMGVGGVLSMGCTV
                     GQAITGFSTLALGSVLTFAAIVGGSALTMKYLYWRAMQD"
BASE COUNT         1122 a         1845 c         1657 g          992 t
ORIGIN      
        1 atcaaggacc tcgtcgcgct gatcctcgac ccgaattcgc cggtcaacag caagtaaggc
       61 gctcctcccc gggcgggccg caatggcccg cccgtgcttt acccttctcc ggagtcatgc
      121 gatgtcgatg aaccgacgcg agttcgtcca gctgctggct gttgcgacag ctggcggcct
      181 gccactttcc cgcgcactgg ccggcgacaa tgcggccggc gcactctacg acctgccgcg
      241 tttcgggcag gtacatctgc tgcatttcac cgactgccac gcgcagttga tgccgatcta
      301 ctttcgcgaa cccagcgtca acatcggcgt cgccgacgcc cgcggcgccc cgccgcatct
      361 ggtcggcgaa catctgttgc gccacttcgg catcaaatcc ggcagccgcg aagcgcacgc
      421 ctttacctac ctggacttcg ccgaggccgc aaaccgcttt ggcaaggtcg gcggctttgc
      481 tcacctcgcg acactggtcc ggcgcctgcg cgcgacgcgg cccggagcgc tgctgctcga
      541 cggcggcgat acctggcagg gatccggcct gtcgctttgg accaatggcc aggacatggt
      601 cgacgcctgc aagctgctcg gcgtagacgt gatgacaggt cactgggagt tcacgctcgg
      661 tgccgatcgc gtcaaggaga tcatcgacaa ggacttcaag ggaacgctcg atttcgtcgg
      721 ccacaacgtc aagacgctcg acttcggcga cccggtcttc cagccctaca cgctacgcga
      781 gatgaacggc gtgtcgattg cggtgatcgg ccaatgcttc ccctacacgc cgatcgccaa
      841 cccgcgctgg caggttcctg actggacctt tggcattcag gaagaggcgc tgcagccggt
      901 gatcgacgag gctcgcggca agggcgcgca ggtcgtcgtc ctgctctcgc acaacggcat
      961 ggacgtcgac ctcaagctcg ccggtcgcgt caccggcctc gacgccatcc tcggcggcca
     1021 cacgcacgac ggcattccgg cgccgatttc ggtcaagaac gccggtggcc agacgctggt
     1081 caccaacgcc ggctcgaacg gcaagttcct cggcgtgctc gatttcgagg tgaagaacgg
     1141 ccgcattgcg gattttcgtt acaagctgct gccggtcttc tccaacctgc tgcccgccga
     1201 tgccgaaatg gccgcctaca tcgaacgcgt gcgcgcgccc tacgcggcga aactcgccga
     1261 gccgctggcg gtgagcgagg aactgctcta tcgacgcagc aatttcaacg gcaccttcga
     1321 ccagctgatc ctcgatgcgc tgatggaggt caagggcgcc gagattggct tctcgccggg
     1381 cttccgctgg ggaacgacgg tgctgcccgg gcagacgatc acgcgcgagc atctgatgga
     1441 tcagacagcg attacctatc catggtctac gctgaccgat atgagcggtg aaatgatcaa
     1501 gaccatcctc gaggacgtct gcgacaacct attcaatccc gacccctact accagcaggg
     1561 tggcgacatg gttcgcgtcg gcggcatgag ctacacctgc gcgcccaacg agaagatcgg
     1621 caatcgcatt caggacatgc gcctcaacgg caagccgatc gacgcgaaga agacctacaa
     1681 ggtggccggc tgggcgccgg tcgccgaggc ggcgcgcgat gccggcggac aaccgatctg
     1741 ggaggtcgta gaaacctggc tcagagccaa gaagaccatc gcaccgcgca agctgaacgt
     1801 gcccaggcta agcggcgtcg ccggcaatct gggaatcgcc tgaactcggg cggtcgccgc
     1861 attctgcagt ccataccaac cacagacaga caaaggagca ccatcatggg gagactagat
     1921 tccaccgtcg tcgccggaag cctgattgcc gcgacgctgc tcgcctcggg cctggccacg
     1981 gccgccgaga agctgaccac gcaggcggcc acattaacga ccggcgcggc actgaaagtc
     2041 gcgcagaccg cgctcgccga ttgtcagaaa ggcggttacg tggtcgccgt cgccgtcgtc
     2101 gatcgcagcg gcatcccgct ggccgtgctg cgcgacaacc tcgccggtcc gcacacgcca
     2161 gaaacggcga tccgcaaggc atggaccgcc gtcagcttcc gcaacaacac gagcagcctg
     2221 ctcgacctct cgggaccggg caagccgtcc tccggcattc gcgacatccc gggcgcgatc
     2281 gttctcggcg gcggcctgac catcaacgcc aaagggttgc tgcttggcgg tgtaggcgtc
     2341 tctggcgcac cgggcgggca tctcgacgac gcctgcggca acgccggaat caaggcaatc
     2401 caggatgcgc tcgaacttga gtagcctccc gcacgacaaa aaaacggcgc cttcgggcgc
     2461 cgttttcatt cgaccgtgtc tctctacttg aactgccggg cgtaagccat accgaaagag
     2521 tcttgagcca tccgcaagtc gatacccgct gtcgaacccg taccgcgcac cgtgttctcg
     2581 aacgcgtgca tgtagcccag agtgatctcc gatcggtctt caagcttgaa ggtcgctcca
     2641 agcgtcaagt gatcctcaac gactccgggt gccagaatgt tgaagaaagt ctcgttgctt
     2701 ggtatcggct gcttgccagt tgcgtaacct gcccgcagaa ccgtgtttcc gttcaacgcg
     2761 tagctgacgc ccactttgta gaccgtcatg tcttgccagc cgaagccagg gccatcggcc
     2821 gcgccaagca cagtagccgc atttccagta ttcgcaatcg atttgacgtc gctgtatttg
     2881 atctccatta catccgcggc gatcatcagg ccagagccgg catcgaacga gatgcccagt
     2941 ccatagttct ccggaatatc aaagtcgccc tgttcggcaa acaggccggc gtacttgtcg
     3001 aacttagaca tgtccgtctt tgactgccaa gtcgcgccaa gctttactcc cggcgccagt
     3061 tctcctagcc agcccaagcg cacgccccac ccggtactgt catcgtagcc aggattgtta
     3121 ataccgaaat tctgtagacc ctcggccttg aaacgctggt aggcaaagtt aatgctggcg
     3181 ccgacgctgt gggcggagtt gatcttaaat gacacggtcg gcgcaatgaa cagctgggcc
     3241 aagtcgacac caacaggttt gttcgcccca aattgcggca cgaacccatt gccgacgccg
     3301 ttttcgtagc tggtgttcat gcccccattg ccgtagatcg aaagccccag cgacatcgtc
     3361 gagtcaaacg ttcggttgta gccaaactcc ggaatgaaga aattctcgtc gccgttgcca
     3421 tcgaacgttc cggcggcggg cccgaatccg gatcccaaag tagcatcgcg atccggccgg
     3481 aacagattca aaccgatgtc gtagcgatcc ccgacccaag ccatgccggc ggggttgctc
     3541 gccgcggcga gtccgtcctg cggcagcgcg atgccgacac cgcccatgcc gatcgatttc
     3601 atgccatagc cgtgcgcgaa gtagccgacg gtggcagagc tgatgcctgg aacaagcgcc
     3661 aaagcggtca gggcagcaat tctcttaagc ttcatgagtc tgtttctcct ctgtggtttt
     3721 tctgacaacg atggcgttgc ctgccaagcc tcccgtctgc actgccactt ccgacggacc
     3781 catctaggct gattcaagtg tcgggccctg ccgtccgctc gaaccatccc cctaaagagc
     3841 aatatcagcc tacccttaag tagggtttgt taattgactt cgatcaaacc acgacaaggc
     3901 aaccttgtgc atccggtcaa tcctgcattg cccgccagta gaggtacttc atcgtcagtg
     3961 ccgatccgcc gacgatggcg gcgaaggtca gcaccgagcc cagcgccagc gtcgagaagc
     4021 cggtgatcgc ctgccccaca gtgcacccca tcgacagaac gccgccaacg cccatcagca
     4081 cgccgccgac cagatggttg ataacgtctt cacgtccgtg gaaccactcg atgcggaact
     4141 gtttcttgaa cagcgcgtaa acgagcgagc cgagaatcac gccgctcagc gccatgattc
     4201 cgaagttaat gaagctcagc ttggtcggct ccatcaggta gcgcgccgaa tcgcccatcg
     4261 ggctgatgaa ggtataggac tgcaccatca ccctgctcgg cgcctcggtg gcgaaatcgg
     4321 cccattcctt ccaggctgcg cccattcgcc cgccactgat ccaccagccg gcaacgacgg
     4381 cgaggccgat gacgctaccg ccaaggatgt tgtcgaagct cttgcggaac tcgtgctgcg
     4441 agaagatgaa ggccagcatg ccgagtgcga tcaacgcgcc cagcccgtag tgaaagccct
     4501 tgccttcgat gccgaaccag ccgccgagaa tcgccggcac ggtctggctg ctcatgccgc
     4561 tggcgcccag attgatcgtg gtcgccgaga tccacgagtc gaacgcggcg ccgtagagat
     4621 cgccccagag catctgatag gcaaagaaag aagcgacggc cagcaccacc agcgacttga
     4681 ggttgccgcc gccgatgcgc accagcgtct tgtttccgca gccgccggcc agcgtcatgc
     4741 cgatgccgaa catgaaaccg ccgagcaggt agcgcagcca ggcgaaattg gccgtgcgat
     4801 aaggcgggaa gacctcgctg tcgaggctta cggtaccgga gccctcgagg acaagtacgc
     4861 cggtgagtgc cagcgccatc gccagcaacc aggcgcgaag ccgtccggtg tcgcccatgt
     4921 tgacccagtc ggacacggcg cccatcgtgc agaaattggt cttgttggca accgcaccca
     4981 tgacgagcgc aatcaggaat accagcagca gcacctgatt atgtatggtg aattccatcc
     5041 cccctccttg cgccggcagc ttatgtgcgg aaccgacagg caattctaag cgggtgacga
     5101 cgccaggaga attacaccaa tcggggatac ccgttcgggt gtccccgccg ggtcggatac
     5161 caggaagacc tcaggcggcg agatcggcga aacagaacag cgtgtcggct ccgatcgcgt
     5221 cgcaaaacac aatggtcagc acggcaccgc gggagcgggc gtaggcctgc acgccgccgg
     5281 ttggctgggc ggcaccacac gcgctgacgc cacccggtga ctcaagttgc cgcgccgcgt
     5341 cgtatcagga gagcagcatc ggttcggcca gccgttctcc ggctagcgcc gtggccgcac
     5401 gcagctgggc ggcgaattcc ggcgccgaat ggtcgttgag gaaaatggtc tgcatgcgtt
     5461 tggtgatgcc actgccccgc tcaggcactg gccctgatac gccaaacgcc gggtgaaccg
     5521 gcgtttggag agtcactgcg gcggcgcttc gccttagggc ttgatcatcg cgtagcccat
     5581 gcgctgcagg cgggcgactt cggcgacacc ggctgg
//
LOCUS       DATDAA010000258         5096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_158378,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000258 DATDAA010000000
VERSION     DATDAA010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158378"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(514..1635)
                     /locus_tag="VIS73_08225"
     CDS             complement(514..1635)
                     /locus_tag="VIS73_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEY5763180.1"
                     /translation="MLNELFALTGFARLTPQMLVMWVIIAGLLYLAVRKNFEPLLLVP
                     IAFGALLANLPTEGMVNPPVGDHPGGLFHYISQGIHLELFPPIIFLGVGALTDFGPVI
                     ANPRTFLLGAAAQFGVFATFIGATLIGFSTDQAAAIGIIGGADGPTSIFLANKLAPEL
                     LAPIAVAAYSYMALVPLIQPPIMRALTTPAERAIRMRSLRQVSRTEKLIFALVVTVLV
                     ILLVPAASALIGMLMLGNFLRECGVTERLSKAAQNEIINIVTILLGTSVGITMTGERF
                     LQVETLKILALGIVAFSISTASGVIMAKLMNLVSKEKINPLIGSAGVSAVPMAARVSQ
                     VEGQKADPGNYLLMHAMGPNVAGVIGTAIVAGYFIARLS"
     gene            complement(1688..2074)
                     /locus_tag="VIS73_08230"
     CDS             complement(1688..2074)
                     /locus_tag="VIS73_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY5763181.1"
                     /translation="MRNFRITVDGTAYEVCVEELESGDKPTPAPRAAVAPPRSAPPAA
                     APPPPVAPRPAAGSPGDILSPLAGTVVKVETSVGQQISQGQNLIMLEAMKMNTYVTAT
                     RAGTVRAIHVAAGASVAEGQPLLSVE"
     gene            complement(2071..2478)
                     /locus_tag="VIS73_08235"
     CDS             complement(2071..2478)
                     /locus_tag="VIS73_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HEY5763182.1"
                     /translation="MLDSLAFILSGFAVVMGALALLWAVSALIGRAFTIAATANTTPR
                     PPTPAAAPGVPAAHVAAISAAVAVITGGRGRVVAVRAPADVANAWALGGRANQLASRR
                     ERGNWAHLPATRTRSDSSSPQSKPPPDSGRRKP"
     gene            complement(2478..4031)
                     /locus_tag="VIS73_08240"
     CDS             complement(2478..4031)
                     /locus_tag="VIS73_08240"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEY5763183.1"
                     /translation="MAIDKNLLDELERRQAEALASGGPDRIAKRHAKGQLTARERLEG
                     LYARDTFQEFGLHAQHETRGFGMDGKSLPTDGVITGIGFIDGRPVAGFSQDFMVAGGS
                     LGRVHARKLCDLMDRAARSGMPVIGFNDSGGARIQEGVESLSGYGQVFNRNVLLSGVV
                     PQIAVICGPCAGGAVYSPALMDFLIMTRHSAHMFICGPEVIRAVTGQQTTKEEIGSAD
                     AHAIESGNIHFIAEDDAHALTLVRELLAYLPSNNMTDPPHRLDEEITLRDDLGIEELI
                     PSDPKMPYDVKEVIKSLSDEGSFLEVQKEFAANMVIGFGRIDGVVVGFVANQPTVKAG
                     ALDINASDKASRFIRFCNIFNIPLVTLVDVPGFLPGVAQEKQGIIRHGAKMLFAYGAS
                     TVPKITVIMRKAYGGAFLAMCSRDMGADLVLAWPTAEIAVMGAEGAVNILYRKEIEEA
                     EDSPARRAELIDAYRTEFASPYQSAARMFVHDVILPRRTKSAVSLGLRTLLSKRETRP
                     PKKHGNIPL"
     gene            complement(4325..4945)
                     /locus_tag="VIS73_08245"
     CDS             complement(4325..4945)
                     /locus_tag="VIS73_08245"
                     /inference="COORDINATES: protein motif:HMM:NF019960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY5763184.1"
                     /translation="MARKTKEEAQETRNRILDAAETVFQARGVSHTTLAEIASEAGVT
                     RGAIYWHFENKVDLFDAMIQRVLDPIELKLAEFQSNAGENPVDALRAIALYFLDRVAK
                     EPAYMRVLEITWHKCEYVGEMATIRDNHLECGNRYLDVLTEGFKLARKRGFLPASTDP
                     RYAAVGLMALVDGLVVNWTLDNKLFRLDRAAPAIIDSYLAGLAKGS"
BASE COUNT          886 a         1509 c         1764 g          937 t
ORIGIN      
        1 ggaggagatt tcgaatgctt cctctcgtgc aaccccatgg gtggccctct caaacgtcct
       61 ctctagtcaa taccgatcgg ggtttggcgg tcgagttcgg tttgtctctc tagctgttga
      121 atgcgcacac gtaaccagcg acatcacgcc aattctgagc gacagcacgc cgaatggaag
      181 cggccgctcg gtttgcacga cgtaaaaggc cgctaaggcc gaggagtact cggacagtca
      241 tcgctcgatg cgactgttcc gctcagaaac cggtcgttgg atttccgcag cggcgaatgt
      301 ctctttgggg tctaggtggt ctgtcaaggc gccgggttgt tggacgatct tcaacactcg
      361 gatttaccga aactcgagtc ccataaggtc gagcagtctg caatcggatt aactggtcaa
      421 gggtcggtta gtcgatcttt gccgcgctgc aagcggcgaa ccgctgcatc gaactgcagg
      481 gccgatcatg gtgatgcttt cacaagcgct tgcttagctc aggcgcgcga tgaagtaacc
      541 ggcgacgatc gcggtgccga tcaccccggc gacgttgggt cccatggcgt gcatgagcag
      601 gtagttgccg ggatcggctt tctgtccctc gacctggctg acgcgtgccg ccatcggcac
      661 ggccgatacg ccagccgatc cgatcagcgg attgatcttc tccttcgaaa ccaggttcat
      721 cagcttggcc atgattacgc ccgaggcggt cgagatcgag aaggcgacga ttccgagggc
      781 gaggatcttc agcgtctcaa cctgaagaaa gcgttcgccg gtcatggtga tgccgaccga
      841 ggtgccgagc aggatcgtaa cgatgttgat gatctcattc tgggcggcct tgctaagcct
      901 ttcggtgacg ccgcattcgc gcaggaaatt gccgagcatg agcatgccga ttagcgccga
      961 ggctgccggc acgagcagga tcaccagcac cgtcaccacc agcgcgaaga taagcttttc
     1021 ggtccgcgac acctggcgca gcgagcgcat gcggatcgcg cgctcggcgg gggtcgtcag
     1081 ggcgcgcatg atcggcggct ggatcagtgg caccagcgcc atgtaggagt aggcggcgac
     1141 cgcgatcggc gcgagcagct cgggggcgag cttgttggcg aggaagatcg aggtcggccc
     1201 gtcagcccca ccgatgattc cgatcgccgc cgcctggtcg gtcgagaagc cgatcagcgt
     1261 ggcgccgatg aaggtcgcga agactccgaa ctgggccgcg gcgccgagca ggaaggtacg
     1321 tgggttggca atcaccgggc caaagtcggt gagcgcgccg acgccgagga agatgatcgg
     1381 cgggaagagt tcgaggtgga tgccctggga gatgtaatgg aacagcccgc cggggtggtc
     1441 gccgaccggc ggattgacca ttccttcggt gggcaggttg gcgagcagcg ccccgaaggc
     1501 gatcggcacc agcagcaggg gttcgaaatt cttgcgcacc gcgaggtaga gcagtccggc
     1561 gatgatcacc cacatgacca acatctgcgg ggtcaggcgg gcgaagccgg tgagcgcgaa
     1621 aagctcgttc aacatgtcca tgcgttggcc tccggcgagg gcgcgcgggt gcgcggggga
     1681 ggaggcgtca ttccacgctg agcaggggtt gcccctcggc gaccgaggcg cctgcggcga
     1741 catggatcgc gcgtaccgtg ccggcgcgcg tagcggtgac ataggtgttc atcttcatgg
     1801 cttcgagcat gatcaggttc tgcccctggc tgatctgttg gccgacgctg gtttcgacct
     1861 tgaccacggt gccagcgagc gggctgagga tgtctccggg ggagccggcg gcgggccgcg
     1921 gtgcaaccgg cggaggcggg gcggccgcgg gcggcgcgga ccgaggcggt gcgaccgcag
     1981 cgcgtggtgc aggggtcggc ttgtcgccgg attcgagttc ctcgacgcag acttcgtaag
     2041 cggtgccgtc gacggtgatc ctaaaatttc tcatggcttc ctcctgccgc tgtcgggcgg
     2101 cggcttggac tgtggactgc tggaatccga tcgggtccgg gtggcgggca gatgcgccca
     2161 gtttcctcgt tcacggcgcg aggcgagctg attggcacgg ccgcccagcg cccacgcgtt
     2221 ggcaacatcg gcaggcgcgc gcaccgcgac gacgcgtccc cgcccgccgg tgatgaccgc
     2281 gaccgcagcc gaaatcgcgg cgacatgggc ggccgggacc cccggtgcgg cggccggggt
     2341 tggcgggcgg ggagtggtgt tcgcggtcgc ggcgatcgtg aaggcacgcc cgatcagcgc
     2401 gctcaccgcc cacagcagtg ccagtgcgcc catgaccacg gcaaaaccgg aaaggatgaa
     2461 ggccagagag tcgagcatca gagcgggatg ttcccgtgct ttttcggcgg gcgggtttcg
     2521 cgcttggaga gcagtgtgcg cagaccgagc gagaccgccg acttggtacg tcgcggcagg
     2581 atgacgtcgt ggacgaacat ccgcgccgcc gactggtagg gcgaggcgaa ttcggtgcgg
     2641 tacgcgtcga tgagttccgc gcggcgggca ggactgtcct cggcctcctc gatttccttg
     2701 cggtagagga tgttgaccgc gccctcggcg cccattacgg cgatctcggc cgttggccag
     2761 gcgagcacaa ggtcggcgcc catgtcgcgc gagcacatcg caaggaaggc gccgccgtag
     2821 gccttgcgca tgatcacggt gatcttgggc acagtcgacg cgccataggc gaagagcatc
     2881 ttggcgccgt ggcggatgat gccctgcttt tcctgcgcaa cgcccgggag aaagcccggg
     2941 acgtcgacaa gcgtgaccag cgggatgttg aagatgttgc agaagcggat gaagcgcgag
     3001 gccttgtccg aggcgttgat gtcaagcgcg ccagccttga ccgtcggctg gttggcaacg
     3061 aaaccgacga cgacaccgtc gatccgcccg aagccgatca ccatgtttgc ggcaaactcc
     3121 ttctgcacct caaggaagct gccctcgtcg gagaggcttt tgatcacctc cttgacgtcg
     3181 tagggcatct tgggatcgga cgggatcagt tcctcgatcc cgaggtcgtc gcgcagcgtg
     3241 atctcctcgt cgaggcgatg cggcgggtcc gtcatgttgt tcgagggcag gtaggcgagc
     3301 agctcgcgca ccagcgtgag cgcgtgggcg tcgtcttcag cgatgaagtg aatgttgccg
     3361 gactcaatgg cgtgggcgtc ggccgagccg atctcttcct tcgtggtctg ctggccggtc
     3421 acggcgcgga tcacctcggg cccgcagatg aacatgtgcg ccgaatggcg ggtcatgatc
     3481 aggaaatcca tcaaagccgg cgaatacacc gcgccccccg cacagggtcc gcagatgacc
     3541 gcgatctgcg gcaccacgcc cgagagcagg acattgcgat tgaagacctg accgtatccc
     3601 gagaggctct cgacgccttc ctggatgcgc gcgccgcccg agtcgttgaa accgatcacc
     3661 ggcatgccgc tgcgtgcggc gcgatccatg aggtcgcaga gcttgcgtgc gtggacacga
     3721 ccaagcgagc cgccggcgac catgaaatcc tggctgaagc ccgccaccgg gcggccgtcg
     3781 atgaagccga tgccggtaat tacgccatcg gtggggagcg acttgccgtc catgccgaag
     3841 ccgcgcgttt cgtgctgggc gtggaggccg aattcctgga aggtgtctcg cgcgtagagc
     3901 ccctcaaggc gctcccgtgc cgtcagctgg cccttcgcgt ggcgcttggc aatgcgatcg
     3961 ggccctcccg aagccagcgc ctccgcctgt cgtcgctcga gctcgtcgag caggttcttg
     4021 tcgatcgcca tggaacccct tcatcactgc cggacggttt gcgagcaacc gtcgtgcggc
     4081 ctcgcagggg catacactag cacgcggacg gcatgggaaa ggcggcgcgc ggggtcttcc
     4141 atgcgcgcgg ttcagctccg gcgcgtgctt gaaacgcccg tcttcctttg cgcacaacga
     4201 tgagaccgat tccgtggaaa gtgctgccca gcgggcggga acggaagacg gttccgttag
     4261 gtatcgagcc gcgcatcggc gcggcggagt tgcgaaggat gaggtgggcg cggtggcttt
     4321 cgcgtcaact tcccttggcc agtccagcca gatagctgtc gatgatcgca ggcgcggcgc
     4381 ggtccaggcg gaacagcttg ttgtcgagcg tccagttgac caccagtccg tcgaccagtg
     4441 ccatcaggcc aaccgcggcg taacgcgggt cggtggaggc gggcaggaag ccacgtttgc
     4501 gggcgagctt gaaaccttcg gtgagcacgt cgaggtaacg gttgccgcat tcgagatggt
     4561 tgtcgcggat cgtcgccatt tcgccgacgt attcgcactt gtgccaggtg atctcgagca
     4621 cgcgcatgta ggcgggctcc ttggcgacgc ggtcgaggaa gtagagggcg atggcgcgga
     4681 gtgcgtccac gggattttcc ccagcgttcg actggaactc ggcaagcttg agttcgatcg
     4741 gatcaaggac gcgctggatc atcgcgtcga aaaggtcgac cttgttttcg aaatgccagt
     4801 agatcgcgcc gcgggttacg ccggcttcgc tggcgatctc ggccagcgtc gtgtggctga
     4861 cgccgcgcgc ctgaaatacg gtttccgccg catcgaggat gcgattgcgg gtttcttgcg
     4921 cttcttcctt ggtttttcgg gccaacggaa ttctccgggt gattcagggt cgcttcaatg
     4981 acgcatgcga tctggcgacc gaacgtgttg cggattctat ctccgtattt acaaacatgc
     5041 acgcatgtat gtaagattcg cgggctcgaa tcgattctag gcgcgctttg ctggcg
//
LOCUS       DATDAA010000259         2094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_368738,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000259 DATDAA010000000
VERSION     DATDAA010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368738"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VIS73_08250"
     CDS             <1..451
                     /locus_tag="VIS73_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763185.1"
                     /translation="NENWDLYLAASWLDTEATGVQRVCDGETVDSCEGSPLFWAPEWA
                     GAAVLSADFPLSRGSISASLEGFWEGERGGGWGAFPETKIDSNLIASFRLDYVSDGNW
                     QAGVYVENLTDEFTYDGQNNNGGILPSHFFGHRMPRTAGVRFRYSWE"
     gene            complement(567..1250)
                     /locus_tag="VIS73_08255"
     CDS             complement(567..1250)
                     /locus_tag="VIS73_08255"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEY5763186.1"
                     /translation="MKTSKYLLAAGALLALVASPALAYEAGTWVFRAGVGVVAPDSDN
                     LVIDDGVDRITVNVDDGTSLTLMGTYMFTRNWGFDILASWPFNHDINVSIVDSVDPGF
                     SPVSAKIAETDHLPPTFSIQYHFLPDSTFQPYVGAGVNYTTFFSTDVEQALVDLGIDD
                     IDLDDSFGLALQFGGDFLFSDRWLINVDVRWIDIESDLEATALGETGELGTVKIDPWV
                     YSVNLGYRF"
     gene            complement(1411..>2094)
                     /locus_tag="VIS73_08260"
     CDS             complement(1411..>2094)
                     /locus_tag="VIS73_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY5763187.1"
                     /translation="GKLKPFGLMHVEFDGFELMVSRTGFTGDLGYELWTAPENAETLW
                     DYLFAAGEATGIRAMGTDALELARIEAGYIMAYIDFLPANATVRTGRTRSPFELGLDW
                     LVDFKKPNFNGRRALAEEKRSGSTWRLVKLDIEGNKPAHHAYIFASAKGGRNIGFVTS
                     AAWSPVCKQNIALGSVRMPHGAPGSTLWVEIFYQREMHWSRVMARAEVVDKPFWFPKR
                     RGLAPPELV"
BASE COUNT          395 a          632 c          669 g          398 t
ORIGIN      
        1 caacgagaac tgggacctgt acctggcggc aagctggctc gataccgagg caacgggcgt
       61 gcaacgcgtt tgcgacggcg aaacggtgga ctcctgcgaa ggcagcccgc tgttctgggc
      121 tcccgaatgg gccggtgccg ccgtgctttc cgccgacttc ccgttgagca ggggctcgat
      181 cagcgccagc ctggagggat tctgggaagg cgaacgcggc ggcggctggg gtgctttccc
      241 ggaaacgaag atcgactcca acctgattgc atcgttccgg ctcgactacg tctcggacgg
      301 caactggcag gcgggcgtct acgtcgagaa cctgaccgac gagttcacct acgacggcca
      361 gaacaacaac ggcggcatcc tgccttcgca cttcttcggg caccggatgc cgcggacggc
      421 cggtgtgcga ttccgttaca gctgggaata gccgggacag cggcttggac tgaaagggcc
      481 ggggcgcagt cgcgccccgg ctttttttgt ggccggtgac ccggcgtacg gatgatcgcg
      541 cttcgggaag cgctcctgcg gcttgcctag aagcggtagc cgagattcac cgagtacacc
      601 cacggatcga tcttgaccgt accgagctcg ccggtttctc caagcgcggt tgcttccagg
      661 tcggactcga tgtcgatcca gcgaacgtcg acgttgatca gccaccgatc actgaacagg
      721 aaatcaccgc caaactgcag cgcaagaccg aatgaatcgt cgaggtcgat atcgtcgatg
      781 ccgagatcga cgagtgcctg ctctacgtcg gtgctgaaaa acgtcgtgta gttcacgccg
      841 gccccgacat aaggttggaa cgtgctgtcc ggaaggaagt ggtactggat cgagaacgtc
      901 ggcggcaggt gatcggtttc ggctatcttc gcactgacgg gactgaagcc cggatcgaca
      961 ctgtcgacga tcgagacatt gatgtcatgg ttgaacggcc aggacgcaag gatgtcgaat
     1021 ccccagttcc gagtgaacat gtaggtaccc atcaacgtca ggctggtgcc gtcatcgaca
     1081 ttcacggtta tcctgtctac gccgtcatcg atgaccaggt tgtccgagtc cggagcaacc
     1141 acgccgacgc cggcacgaaa tacccaggtg ccggcttcat aggccagtgc gggtgatgct
     1201 actaatgcca gcagggcacc cgctgcaagc agatatttgc tggttttcat ggtgatttcc
     1261 tttagtccgc gaagggactg ttaaacattg caatgttttc cctggacttc ggatgagtcc
     1321 acgcgcgtat cttaggcctc gccgcgctgc agcaagtcag gagttcatgc agtccggctg
     1381 cggcgaatac gtagagaccg gtttttgcgc ctagacgagt tccggcggcg cgagcccgcg
     1441 gcgcttcggg aaccagaacg gcttgtcgac gacctcggcc cgggccatga cccgcgacca
     1501 gtgcatttcg cgctggtaga agatctccac ccacagcgtc gagcccggcg caccatgcgg
     1561 catccgcacg ctcccgagcg cgatgttctg cttgcagacc ggggaccagg cggccgacgt
     1621 gacgaaaccg atgttcctgc cgcctttcgc gcttgcgaaa atgtaggcat ggtgggcggg
     1681 cttgttgccc tcgatgtcga gtttcacgag ccgccaggtc gagccgctgc gtttctcctc
     1741 ggccagcgcg cggcgcccgt tgaaattggg cttcttgaaa tcgaccagcc agtcgaggcc
     1801 aagctcgaac ggcgagcgcg tgcggcccgt gcggacggtc gcgttcgccg gcaggaagtc
     1861 gatgtaggcc atgatgtagc cggcctcgat gcgcgccagc tccagcgcat cggtgcccat
     1921 ggcgcgtatg ccggtggctt cgccggcggc gaacaggtaa tcccagagcg tctcggcgtt
     1981 ctcgggggcc gtccacaact cgtagccgag gtcgcctgtg aatcccgtgc gcgaaaccat
     2041 gagttcgaac ccgtcgaatt cgacgtgcat gagcccgaaa ggcttgagct tgcc
//
LOCUS       DATDAA010000260         2880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_158528,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000260 DATDAA010000000
VERSION     DATDAA010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158528"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VIS73_08265"
     CDS             complement(<1..704)
                     /locus_tag="VIS73_08265"
                     /inference="COORDINATES: protein motif:HMM:NF040455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763188.1"
                     /translation="MRAVSKHALAILLVVAGLAAFSAEPGVQEAIRTEIEQLRETGRL
                     STGGIEVASGKLLARNYENRNFEPAWPDVESVNELIRLVESTADDGLDPADYHFEALD
                     RVRARLAAGERFDAAEQAAIDIGMTDSLVRIGYHRRFGKVNPNDLDPDWNFQRELRRR
                     DPAIVFQEAMDADSLAGYLERTFPRDKLYRRMQGYLVQYRDLAASGGWPRIPDGPTIR
                     PGDGDPRLSVLAQRLA"
     gene            809..1348
                     /locus_tag="VIS73_08270"
     CDS             809..1348
                     /locus_tag="VIS73_08270"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEY5763189.1"
                     /translation="MTKHSKRLLVLSALLSVAVMGSMIKPTKVRAADARQLSFYHTHT
                     RHSLSVIYYQNGEYVDSALDEINRFLKDFRTGDITEINPELLDLLHDVRNELGSEKAY
                     EVISAYRSPKTNEMLRATGSGVAKKSHHVKGNAIDVRLRGVRTKTLRETALRLGRGGV
                     GYYPKSDFVHMDMGPVRQW"
     gene            complement(1364..2302)
                     /locus_tag="VIS73_08275"
     CDS             complement(1364..2302)
                     /locus_tag="VIS73_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763190.1"
                     /translation="MRTSACALMLAAAFLSGNAIGQEMTQLSCKDFVPTQEALDRFPD
                     LAGACEGVVERDGELYGLFRAEVRRVRGNNITLHLPATGHTFTIKPEPSARVLIDGRK
                     VRPRDLMPRDEISIYLSASEFAKPDVQEIAFITESDFMIDVPADDVSTVSIPGRVITS
                     TARREAIVESVNKETREIKVLDANGKIHKFVAGEMVANFDQIEPRDRIVTEYLESVAV
                     FVVPEGTPEMGDMGLVEVAPLGEKPGMAMADTYMIAARVDAVDTDTRIVTLRGKDGFQ
                     TAIRVADDIELANLKVGDEVRMRVTEAVAISVVEAP"
     gene            complement(2360..>2880)
                     /locus_tag="VIS73_08280"
     CDS             complement(2360..>2880)
                     /locus_tag="VIS73_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763191.1"
                     /translation="DKLLVSSPDLDSAAVYDLISEIRRLQPALAAKRPFLFEQLGSEF
                     DASGSTFVLHPGSQAFAVRDEPTFYERYSGVAEVLVTLVIGLISGSYAVIQIYNRRRK
                     NRIDGFYTDVMAIRDSVSEHSSATEREAAVEKVQELQNKAFEMLIREKLAADESFRIF
                     VTLSKDIIAQLK"
BASE COUNT          558 a          932 c          838 g          552 t
ORIGIN      
        1 atcgccagcc gctgcgccag cacggacagg cgcgggtcgc cgtcgccagg acgtattgtc
       61 ggcccatcgg gtatccgcgg ccagccgccc gacgcggcca ggtcgcgata ctgcacgagg
      121 tagccctgca tacggcggta aagtttatcg cggggaaacg tgcgttcgag gtacccggcg
      181 agcgaatcgg catccatggc ctcctggaag acgatcgcgg gatcccggcg ccgcagctcg
      241 cgctggaaat tccagtcggg atcgagatcg ttcgggttca ctttgccgaa acgccggtga
      301 tagccgatgc ggacgaggct gtcggtcatg ccgatatcga tggcagcctg ctccgccgcg
      361 tcgaagcgct cgccggcggc aagcctcgca cgtacgcgat ccagcgcctc gaaatggtag
      421 tctgccggat cgagcccgtc atccgcggtc gactcgacca ggcgtattag ctcgttcaca
      481 ctctcgacgt ctggccaggc cggttcgaag ttgcggtttt cgtagttccg cgccagcaac
      541 ttgccggacg cgacctcgat cccgccggtg ctcaggcgcc cggtttcgcg cagctgctcg
      601 atctcggtgc ggatcgcctc ctggacgcct ggttcagcgg agaacgccgc aagacccgca
      661 accaccagca aaatcgccag tgcgtgcttc gataccgccc tcattccgaa attgcctccc
      721 gccggttttg tgcgctgatt agcggttccc ggccaccatg atgctagaat ctgcaacccc
      781 ataggtatcc gggataaccc tctacacgat gacgaaacac tcgaagcgac tactggtact
      841 gtccgccctg ctcagcgtgg ccgtcatggg cagcatgatc aagccgacca aggtccgcgc
      901 cgcggacgcc cgccagctct cgttctacca cacgcacaca cgccatagct tgagcgtcat
      961 ctattaccag aatggcgagt acgtcgactc ggccctggac gagatcaatc gcttcctgaa
     1021 ggacttccgc accggcgaca tcacggaaat caacccggaa ctcctggacc tgctgcacga
     1081 cgttcgcaac gaactcggca gcgaaaaggc ctacgaggta atttccgcgt accgatcacc
     1141 gaagaccaac gagatgctgc gcgcaacggg tagcggtgtc gcaaagaaga gccatcacgt
     1201 gaagggcaac gcgatcgacg tgcggctacg cggcgtgcgc accaagacac tccgcgaaac
     1261 cgcactccga ctggggcgcg gcggcgtcgg ctactacccg aagtccgact tcgtgcacat
     1321 ggacatgggg ccggtcaggc agtggtagcg ctataaaccc ggatcagggc gcttcgacga
     1381 ccgatatggc cacggcctcg gttacccgca tgcgaacctc gtccccgacc ttcaggttcg
     1441 ccagttcgat atcgtccgct accctgatcg cggtctgaaa tccgtccttg cccctcaggg
     1501 tcacgattct cgtgtccgtg tcgacggcat cgaccctggc ggcaatcatg tacgtgtcgg
     1561 ccatcgccat gccgggcttc tcgccgagcg gcgcgacctc gacgagcccc atgtcgccca
     1621 tctcgggggt gccttcggga accacgaaga cggccactga ctcgaggtat tcggtgacga
     1681 tgcggtcacg cggctcgatc tggtcgaaat tggccaccat ctcgccggcc acgaacttgt
     1741 gaatcttgcc gtttgcgtct agcaccttga tctcgcgggt ctccttgttg acggactcga
     1801 cgatcgcctc gcggcgcgcc gtgctcgtga tcacccgtcc cgggatggac actgtcgata
     1861 cgtcgtcggc cggcacgtcg atcatgaagt cggattccgt gatgaacgcg atctcctgca
     1921 cgtcgggttt tgcgaactcg ctcgccgaca ggtagatgct gatctcgtcc ctcggcatca
     1981 ggtcgcgcgg acgcaccttc ctgccatcga tcaatacacg tgccgagggt tccggcttta
     2041 tggtgaacgt atggccggta gccggcaggt gcaacgtaat attgttgccg cgcacccgcc
     2101 gcacctcggc tcgaaacagg ccgtagagtt caccgtctcg ctcgacgacg ccctcgcagg
     2161 caccggcgag gtcggggaat ctatccagcg cttcctgcgt gggaacaaaa tccttgcagc
     2221 tcagctgcgt catctcctgg ccgatggcgt tccccgacag gaaagccgcc gctagcatca
     2281 gtgcacaggc agacgttctc ataagttttc tcctttttgg gtcgcccgca gcagggactg
     2341 ccgggcgcgt aatcgtcgtt tacttaagtt gggcaataat atccttcgac aaggttacga
     2401 agatgcgaaa actctcgtcg gccgcgagct tctcccggat gagcatctca aacgccttgt
     2461 tctgcaactc ctggaccttc tcgaccgcag cttctctctc ggttgcagag ctatgctcac
     2521 tgaccgagtc tcgaatcgcc atgacatccg tgtagaaacc atcgatgcga ttcttacggc
     2581 ggcggttata gatctggata acggcataac tgccgctgat caggccgatg accagggtaa
     2641 cgagcacctc ggccacaccg gaatagcgtt catagaatgt cggttcatcg cggaccgcaa
     2701 atgcctgcga gcccgggtgc aacacgaatg tcgaacctga cgcatcgaac tcggaaccaa
     2761 gctgctcgaa caggaatgga cgctttgccg caagggcggg ctgcaggcgc cggatttcac
     2821 tgatcaaatc gtaaaccgcg gcgctgtcca ggtcggggct cgaaaccagc agcttgtcga
//
LOCUS       DATDAA010000261         3947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_508133,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000261 DATDAA010000000
VERSION     DATDAA010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508133"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VIS73_08285"
     CDS             <1..898
                     /locus_tag="VIS73_08285"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY5763192.1"
                     /translation="GIIYESRPNVTADAAALCLKAGNAAILRGGSEAIRSNRAIAALV
                     SEGLRAAGLPETAVQVIDTTDRAAVGELIAMPEFVDVIVPRGGKGLIERISREARVPV
                     IKHLDGNCHVYVDGDADLEMATQIVDNAKTQRYGTCNTAESLLVDASIARTALPRLLA
                     PLIAKQVEIRGCEATCALIPQAKPATEEDYGTEFLAPIISVRVVDGIAAAIEHINRYS
                     SQHTEAIVTASYEKATRFLREVDSSSVMVNASTRFADGFEYGLGAEIGISTDKIHARG
                     PVGLEGLTSQKWIVLGQGHIRG"
     gene            944..1549
                     /locus_tag="VIS73_08290"
     CDS             944..1549
                     /locus_tag="VIS73_08290"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEY5763193.1"
                     /translation="MHRLKNTISLLLGVGLLLATPLTRAQGAETISAGGTQFAAKADL
                     DGQPLLANGAGIRSKFFLDIYAAALYLPTKTSDPSAILAAEAPSRVHIVMLRDLSADR
                     FVSALEDGLRDNLTAARYQEIAPQRSELATAMRALGEAKKGDVILLERAQRDSTRLVV
                     NGSPRAEIAGARFFTALLRIWLGDQPVDSALKERLLGRDGR"
     gene            1589..2512
                     /gene="xerD"
                     /locus_tag="VIS73_08295"
     CDS             1589..2512
                     /gene="xerD"
                     /locus_tag="VIS73_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY5763194.1"
                     /translation="MIVSAHPASETRGAEEPLIDGFCDALWLEDGLARTTLDSYRSDL
                     LLLQRLLPRAAPVTQASEAELAQALADFSRTHKRTSQARLLSTLRRFYGWLVVNGLRA
                     DDPSLKLARPARSNRLPKVISEAQVEALLAAPDENTAQGLRDRAMLETLYASGLRVSE
                     LIGLTLGAVNVDMGVARVLGKGSKERLVPLGEMAIESIHRYQREARAKMLNGQVTDAL
                     FVTRRGGPMTRQRFWALIKGYATRAGIDSSKLSPHVLRHAFATHLLNHGADLRVVQLL
                     LGHADISTTQIYTHVARERLKALHARHHPRG"
     gene            2523..3014
                     /locus_tag="VIS73_08300"
     CDS             2523..3014
                     /locus_tag="VIS73_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEY5763195.1"
                     /translation="MQRNEHAPETPATRALRAARVAFSSHLYPYEEHGGTRVSARELN
                     VDEHAVVKTLVFEDEGAKPLIVLMHGDCKVSAKELARQIGCKRVEPCKPEVAHRQTGY
                     LVGGTSPFGTKKRLPVYLESSILDLPLIYLNGGRRGFLVGVHPHDVLVTLQAKPVKVA
                     LDK"
     gene            complement(3039..3443)
                     /locus_tag="VIS73_08305"
     CDS             complement(3039..3443)
                     /locus_tag="VIS73_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763196.1"
                     /translation="MQEFLIQVSSHALLWIIYLALYPMAFIWLRRAYRIIVKRDFSEV
                     ALRRGEPPENPEKYAPFTAATNLVAGTVIGSTAVAVFAVPLPFQTWTAIAGSTLWIKI
                     FADFVISRQAKLKLGRYLKEDKKPADRADGVD"
     gene            complement(3561..>3947)
                     /locus_tag="VIS73_08310"
     CDS             complement(3561..>3947)
                     /locus_tag="VIS73_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEY5763197.1"
                     /translation="GTVALAFGIAASSLFPAIMMGIFSKKMNKEGAIAGMLAGLTITL
                     FYVFAHKGIFFIKGTEFLDVFGGKPDFFFGISPNAFGGIGALVNFAVAFAVDKMTKEP
                     PEHIQHLVESVRIPRGSKAVDGSHAH"
BASE COUNT          743 a         1326 c         1260 g          618 t
ORIGIN      
        1 tggcatcatt tacgaatcac ggccaaacgt caccgccgac gcggccgcac tgtgcctgaa
       61 agcgggcaat gcggcaatcc tgcgcggcgg ctccgaggca atccgcagca accgggcgat
      121 tgccgcgctg gtcagcgagg gactgcgcgc cgcgggactg cccgaaaccg cggtacaggt
      181 gatcgacacc accgaccgcg cggcggtcgg tgaattgatc gcgatgccgg aattcgtcga
      241 cgtgatcgtc ccacgcggcg gcaaggggct gatcgagcgc atctcgcgcg aggcgcgggt
      301 gccggtgatc aagcaccttg acggcaactg ccacgtttac gtcgacggcg atgccgatct
      361 cgagatggca acgcagatcg tcgacaacgc caagactcag cgctacggca cctgcaatac
      421 cgccgaatcg ctgctggtcg atgccagcat cgcccggacg gcactgccgc gcctgctcgc
      481 gccactgatc gccaagcagg tcgagatccg cggctgcgag gcgacctgcg cgctgattcc
      541 acaggccaaa ccggcgaccg aggaggacta cggcaccgaa ttcctcgccc cgatcatctc
      601 ggttcgggtc gttgacggca tcgccgccgc gattgagcac atcaaccgtt attcgtcgca
      661 gcatactgag gcaattgtca cggcaagcta cgaaaaggcg acgcgtttcc tgcgcgaggt
      721 cgattcgagc tcggtcatgg tcaacgcatc gacccgcttc gccgacggtt tcgagtacgg
      781 tctcggtgcc gagatcggca tatccaccga caagatccac gcccgcggcc cggtcggcct
      841 cgaaggcctg accagccaga agtggatcgt gcttggccag ggacacatcc ggggctgagc
      901 ccgatttcgc tcggcgcgtt cgaccctttc acgggaggca agcatgcatc gcttgaaaaa
      961 caccatctcg ctccttctgg gcgtcgggct gctcctggcc acgccactga cccgggcgca
     1021 gggcgccgaa acgatctcgg ccggcggcac gcagttcgcg gcaaaggccg acctggacgg
     1081 gcagccattg ctcgccaatg gtgccggcat ccgcagcaag ttcttcctcg acatctacgc
     1141 cgccgcactt tacctgccga ccaagaccag cgacccatcg gcgatcctcg ccgccgaggc
     1201 gccgtcacgc gtgcatatcg tgatgctgcg cgacctcagc gccgaccgct ttgtatcagc
     1261 gctcgaggac ggactgcgcg acaacctaac cgccgcgcga taccaagaga tcgcgccgca
     1321 gcggtccgag ctggcgacgg ccatgcgcgc gctcggcgaa gccaagaagg gcgacgtcat
     1381 cctgctcgaa cgggcgcagc gcgacagcac gcgactcgtc gtcaacggct cgccgcgcgc
     1441 cgagatcgcc ggcgccagat tctttactgc gctgctgcgg atctggctgg gcgaccagcc
     1501 ggtcgattcg gcgctcaagg agcgcctgct cggacgtgac gggcgctgaa acccatctcg
     1561 tagggccagc gcgagggcgg tcgcgcgcat gatcgtcagc gcgcaccccg caagtgagac
     1621 ccgcggcgcc gaagagccct tgatcgacgg tttctgcgat gcgctgtggc tggaagacgg
     1681 gctcgcgcgg accaccctcg acagctaccg atccgacctg ctgctgctgc aacggctgct
     1741 gccgcgcgcc gcgccggtca cgcaggcgag cgaggctgaa ctggcgcagg cgctggctga
     1801 cttttcgcga acccacaagc ggaccagcca ggcgcgcctg ctgtcgacgc tgcggcgctt
     1861 ctacggctgg ctcgtggtca acggcctacg cgccgatgat cccagcctca agctggcccg
     1921 cccggcgcgc agcaaccggc tgcccaaggt aatcagcgaa gcgcaggtcg aggcactgct
     1981 cgccgcgccc gacgagaaca cggcgcaggg tttgcgcgac cgggcgatgc tggagaccct
     2041 ctacgccagc ggcctgcgcg tttccgaact gattggcttg acgctgggcg cagtcaatgt
     2101 cgacatgggc gtcgcccgcg tgctcggcaa gggctcgaaa gagcggctgg tgccgctggg
     2161 cgagatggca atcgagagca ttcaccgcta ccagcgtgaa gcgcgcgcca aaatgctgaa
     2221 tggacaggtc accgacgcgc tgttcgtcac gcgccgtggc ggcccgatga cccggcagcg
     2281 attctgggcg ctgatcaagg gctacgcgac ccgcgccggc atcgactcgt cgaagctgtc
     2341 gccgcacgtg ctgcggcacg cgttcgcgac ccatctgctc aaccacggcg ctgacctgcg
     2401 cgtcgtgcaa ttgctgctcg gccacgccga catctcaacc acgcagatct acacccacgt
     2461 cgcccgcgaa cgactgaagg cgctgcacgc gcgccaccat ccgcgcggct gacaacgcac
     2521 cgatgcaacg aaacgagcac gcccccgaaa cgccggccac gcgcgctttg cgcgcggcgc
     2581 gggtcgcctt ctcatcgcat ctctacccct acgaggagca cggcggcacg cgtgtctcgg
     2641 cacgagaact caatgtcgat gagcacgcgg tggtgaagac gctggtcttt gaggacgaag
     2701 gcgcgaagcc gctgatcgtg ctgatgcacg gcgactgcaa ggtatcggcc aaggagctgg
     2761 cccgtcagat cggctgcaaa cgcgtcgagc cgtgcaagcc cgaggtcgcg caccggcaga
     2821 ccggctatct ggtcggcggc accagcccct tcggcacgaa gaagcgactg ccagtctatc
     2881 tcgaatcgag catcctcgat ctgccgctga tctacctcaa cggcggccgc cgcggttttc
     2941 tcgtcggcgt gcacccgcac gatgtgctcg tcacgctgca ggccaagccg gtcaaggttg
     3001 cactcgacaa gtaggttctg ctgcgggcgc gcccgcgctc agtccacccc gtcggcgcga
     3061 tccgctggtt tcttgtcctc tttcagatag cggccgagct tgagcttggc ctgccggctg
     3121 atcacgaagt cggcaaagat cttgatccac agtgtcgaac cggcaatcgc ggtccaggtc
     3181 tggaacggca aaggcacggc gaaaaccgcg acggcggtcg acccgatcac ggtgccagcg
     3241 acgaggttcg tcgccgccgt aaagggcgcg tacttctccg ggttctccgg cggctcgccg
     3301 cggcgcagtg ccacttccga gaaatcgcgc ttgacgatga tccgataggc gcggcgcagc
     3361 cagatgaagg ccatcggata gagcgcgaga tagatgatcc agagcagcgc gtggctcgag
     3421 acctgaatga gaaattcctg catggctgtt ccgtgtatcc ggtgacgatg cgttaaaaga
     3481 aaagccccac caggcgaacc gggtggggcc ttggccccaa agcccgcacg cggcgggctt
     3541 gggggcgatt ctactagagc ttagtgggcg tgcgagccgt caaccgcctt ggaaccacgc
     3601 gggatacgca cggattcgac caggtgctga atgtgctccg gcggctcctt ggtcatcttg
     3661 tcgaccgcga aggcaaccgc gaagttgacc agcgcgccga taccgccgaa cgcgttcggc
     3721 gaaataccga agaagaagtc gggcttgcca ccgaagacat cgaggaactc ggtgcccttg
     3781 atgaagaaga tgcccttgtg cgcaaacacg tagaagagcg tgatggtcag gccagccagc
     3841 atgcccgcaa tcgcaccttc cttgttcatt ttcttcgaga aaatgcccat catgatcgcc
     3901 gggaacagcg acgaggccgc gataccgaag gccagcgcca cagtacc
//
LOCUS       DATDAA010000262         3115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_368874,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000262 DATDAA010000000
VERSION     DATDAA010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368874"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..607
                     /gene="accB"
                     /locus_tag="VIS73_08315"
     CDS             140..607
                     /gene="accB"
                     /locus_tag="VIS73_08315"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY5763198.1"
                     /translation="MDLRKLKKLIDLVQESGISELEVTEGEEKVRIAKHIPAPASHAP
                     MPTYYAQPAPAAAPAPTATPAVAVAAPAGDDTPEGHIVKSPMVGTFYRSASPSVPAFV
                     EVGASVNKGDTLCIIEAMKLMNEIESDASGVVKAILIDNGQPVEFGEPLFVIG"
     gene            695..2056
                     /gene="accC"
                     /locus_tag="VIS73_08320"
     CDS             695..2056
                     /gene="accC"
                     /locus_tag="VIS73_08320"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY5763199.1"
                     /translation="MFEKILIANRGEIALRIQRACREMGIKTVVVHSEADREAKYVKL
                     ADESVCIGPAASKESYLNVPRIIAAAEVTDSEAIHPGYGFLSENADFAERVESSGFVF
                     IGPRAETIRMMGDKVNAKDAMKAAGVPCVPGSDGALPEDPKEIVKIARAVKYPVIIKA
                     AGGGGGRGMRVVHTEGALISAVQTTRQEAGSFFNNPAVYMEKYLENPRHIEIQVLADQ
                     HGNAVYLGERDCSMQRRHQKIIEEAPAPGIERKAIHQVGEACAEACRRINYRGAGTFE
                     FLYENGEFYFIEMNTRVQVEHPVTELITGIDIVQEQIRIAYGEKLRFKQKDIRFQGHS
                     IECRINAEDPFTFVPSPGKIEFWHPPGGPGVRFDSHAYSGYTVPPHYDSMIAKVITKG
                     DTREQAIRRMRIALSEMGVQGIKTNIPLHQELMMDARFIEGSTSIHYLEHRLADKGEL
                     KKS"
     gene            2079..2984
                     /gene="prmA"
                     /locus_tag="VIS73_08325"
     CDS             2079..2984
                     /gene="prmA"
                     /locus_tag="VIS73_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY5763200.1"
                     /translation="MSWLSVSLVTDAIHAEALADALAAVGALSSSIEDDAAGTADESP
                     QFGEPGAEATLGWARSRVTALFAPDADLAQMIPAAAAAAGFAETPAYQAETLDDQDWV
                     RQTQAQFEPIRISERLWIVPSWHAAPDPEAINIELDPGLAFGTGSHPTTRLCLEWLVE
                     NVDRGADVLDYGCGSGILAIAAAKLGAGPVVGTDIDPQAVAAARDNAARNGVTIRFES
                     VSAGHDGRYRIVVANILANPLKALAPALCAHVDDGGRLALSGILVEQADELIDTYAPW
                     IALDVAGSREGWVCLAGQKTLPGAN"
BASE COUNT          593 a         1042 c          982 g          498 t
ORIGIN      
        1 gcggtcggtg tcatttccgg actcggcagc gaaggctatc tgctggcgct cgagttcgcc
       61 ctgaatcgcg tcgtccagcc ggactgacgc cacagcgccc gccggcgcgt ccagtacaag
      121 aacacagcaa ggagaaagca tggatcttcg taaattgaag aagttgatcg acctcgttca
      181 ggaatcgggg atttccgaac tggaagtcac cgagggcgag gagaaggtgc gcatcgccaa
      241 gcacattcct gcgccggcaa gtcacgcacc gatgccgacc tactatgcgc aaccggcgcc
      301 ggccgcagcg cctgccccca ccgctacacc ggcagtcgcc gtcgcggcac cggccggcga
      361 cgatacaccc gagggccaca tcgtcaagtc gcccatggtc ggcacgttct accgctcggc
      421 ctcaccgtcg gtaccggcct tcgtcgaggt cggcgccagc gtcaacaagg gcgacacgct
      481 atgcatcatc gaagcgatga agctgatgaa cgagatcgag tccgatgcct cgggcgtggt
      541 caaggccatc ctcatcgaca acgggcaacc cgtcgaattc ggcgagccgc tgttcgtcat
      601 cggctgatcg ccggttacag gttcgtgccg aaaatcccac agatcgacag ccgcggcagc
      661 cagccttgcg ctggcttcgc ggcggaggcc atacatgttt gaaaagatcc tgattgccaa
      721 ccggggggag atcgccctgc gcatccagcg cgcctgccgc gagatgggca tcaagaccgt
      781 tgtcgtgcac tccgaggccg accgtgaagc gaaatacgtc aagctcgccg acgagtcggt
      841 gtgcatcggg cctgctgcgt cgaaggaaag ctacctgaac gtaccgcgca tcatcgcggc
      901 ggccgaggtc accgactcgg aggccattca cccgggttac ggcttcctct ctgagaacgc
      961 cgactttgca gagcgcgttg aatcgtcggg cttcgtcttc atcggtccac gtgccgaaac
     1021 catccgcatg atgggcgaca aggtgaatgc caaggacgcg atgaaggcgg ccggcgtgcc
     1081 ctgcgtgccg ggttcggacg gcgcgctgcc cgaggacccc aaggaaattg tcaagatcgc
     1141 ccgcgcggtc aagtacccgg tgatcatcaa ggccgccggc ggtggcggcg gccgcgggat
     1201 gcgcgtcgtc cacaccgaag gcgcgctgat cagtgccgtg cagaccaccc gccaagaggc
     1261 cggcagcttc ttcaacaacc cggccgtgta catggagaag tacctggaga atccacggca
     1321 catcgagatc caggtactcg ccgaccagca cggcaacgcc gtctacctcg gcgaacgcga
     1381 ctgctcgatg cagcgccgcc atcagaagat catcgaagag gcccccgcac cgggtatcga
     1441 gcgcaaggcg atccaccagg tcggcgaagc ctgtgccgag gcctgccgcc ggatcaatta
     1501 tcgcggcgcc ggcaccttcg agttcctcta tgagaacggt gagttctact tcatcgagat
     1561 gaacacgcgc gtgcaggtcg agcatccggt caccgagttg atcaccggca tcgacatcgt
     1621 tcaggaacag attcgcatcg cctacggcga gaagctacgc ttcaagcaga aggacattcg
     1681 gttccagggg cattcgatcg aatgccggat caacgccgag gacccattca ccttcgtccc
     1741 ctcgccgggc aagatcgagt tctggcaccc gccgggtggt cccggcgtgc gttttgactc
     1801 gcacgcctac tcgggctaca cggtgccgcc gcactacgac tcgatgatcg ccaaggtgat
     1861 caccaagggc gacacccgcg agcaggcaat tcgccgcatg cgcatcgcgc tctcggaaat
     1921 gggcgtgcag ggcatcaaga ccaacattcc gttgcaccag gaactgatga tggatgcgcg
     1981 cttcatcgaa ggcagcacca gcattcacta cctggagcac cggctcgccg acaagggcga
     2041 gctcaagaag tcctgagcgt caacgcggag accgcggcgt gtcctggcta tccgtctcgc
     2101 tggtgaccga cgcgattcac gcggaggcgc tcgccgatgc actggcggca gtcggcgcac
     2161 tgtcgtcgtc gatcgaggac gacgccgcgg gcaccgccga cgagtcgccg cagttcggcg
     2221 agcccggtgc cgaggcgacg ctgggctggg cgcgttcacg agtcaccgcc ctctttgcgc
     2281 ccgatgccga cctggcgcag atgattcccg ctgccgcggc cgccgccggc ttcgccgaga
     2341 cacccgccta ccaggctgaa acgctcgacg atcaggactg ggtccgccag acgcaggcgc
     2401 agttcgagcc gatacgcatc agcgagcggc tgtggatcgt tccctcctgg cacgccgcgc
     2461 ccgatcccga ggcgatcaac atcgaacttg atccgggcct ggccttcggc accggttcgc
     2521 acccgacgac gcggctgtgc ctcgaatggc tggtcgagaa tgtcgaccgc ggcgccgatg
     2581 tcctcgacta cggctgcggc tcgggaattc tcgcgattgc ggcagccaag ctcggtgccg
     2641 gtccggtcgt cggcaccgac atcgacccgc aggcagtcgc cgccgcacgc gataacgcgg
     2701 cgcgcaacgg ggtcaccatc cgtttcgagt cggtcagcgc cgggcatgac ggccgatacc
     2761 gcatcgtcgt tgccaacatc ctcgccaacc cgctgaaggc actggcaccg gccctgtgtg
     2821 cccacgtcga cgatggcggc cggctggccc tgtcgggcat cctcgtcgag caggccgatg
     2881 agctgatcga cacctacgcg ccgtggattg cactcgatgt cgccggcagc cgcgagggct
     2941 gggtctgcct ggccgggcag aagacgttgc cgggagcgaa ctgatggccg tcatcgcccg
     3001 ctgcccgcac tgcgacacca gtttccgcgt tacgcccaag cagatgaagg cgaagagcgg
     3061 caaggtccgc tgcggctcgt gcaaggcggt attcaatgtc ctcgaacacc tgatc
//
LOCUS       DATDAA010000263         5246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_124228,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000263 DATDAA010000000
VERSION     DATDAA010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124228"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..2380
                     /gene="fliD"
                     /locus_tag="VIS73_08330"
     CDS             92..2380
                     /gene="fliD"
                     /locus_tag="VIS73_08330"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEY5763201.1"
                     /translation="MAISSPGIGSSLDVNSIVSQLMALEQRPLTLLGIKEARLQSRLS
                     AIGQVKGALSSIQSGLANLKDASRFSSFKGASSDATVASVTASTFAEPGTYNLDVTQL
                     ASAQKLNSQAFATAETVVGGGTLTLQFGSFNGASFTANSEKSAINLTLAADATLASVR
                     DAINGAKAGVTASIVNDGTGFRLAISSSQTGAAFGLKITASDLDGNNTDDAGLSRLVF
                     DPAGTKRLNESQAARDAAFTIDGIAISQAGNTVTNALSGVTLNLIKVGTSTLSVSRDT
                     SAVKSAIDGFVKAYNDGNKVLTQLGAFNAETREAGALQGDSTLRTIRGQMRSLLGGTL
                     PFASGGLRNLSEIGLSFALDGSLTLDASKLQAVLDDPTKDVASLFASVGRPSDPSIRF
                     GSATNAVKPGDYALSVTQLATRGTITASSLLNGVVAAGINDSVGLTVNGSVVNVSLSA
                     GSYSAAGFAAELQSRINGALSGTAASVAVTATGTGGSLAGSAAAPLLIDATNNTIDVT
                     LNGVTHTVTLTQDTYLSASAVAAEIQAKTNAAFAADGFAVAVTEAGGVLTISAANSFG
                     TGSSIAVNGLGADALLGVARTATAGAAGTLSITSTNYGSTSTLAGLSVDASLVTPGSI
                     DTASASGVDAAGTLGGASATGSGQVLNAAGLSVVVTGGATGNRGSVSFDRGFASQLDT
                     LLTSALSSTGLLESRVQGIQSSIDSIGDNRELIERRLVSIEARLRRQFTALDTLIAGL
                     SQTSNFLQQQLATLPGASSGNS"
     gene            2397..2822
                     /gene="fliS"
                     /locus_tag="VIS73_08335"
     CDS             2397..2822
                     /gene="fliS"
                     /locus_tag="VIS73_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HEY5763202.1"
                     /translation="MFGNYNAVSAYKSVGVDSAVTAADPHKLILLLLDGGLSAISIAK
                     GAMAQGQVAGKGEAISKAIDIVENGLRASLDKEQGGELALQLDLLYEYMGRRLLHANV
                     KNDSAALDEVSALLREIHAAWSEIRKRAIDQAANPAAAA"
     gene            2833..3132
                     /locus_tag="VIS73_08340"
     CDS             2833..3132
                     /locus_tag="VIS73_08340"
                     /inference="COORDINATES: protein motif:HMM:NF017237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HEY5763203.1"
                     /translation="MNSGDCWSQLADSAQRMANAAQSHDWDALIDQYGRFAAIAENLP
                     RHQLPLPSDAARREREQLIRRVLEDVEAVREQVGPWMTDVRPLLEAWRRSETPAP"
     gene            3129..4418
                     /locus_tag="VIS73_08345"
     CDS             3129..4418
                     /locus_tag="VIS73_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEY5763204.1"
                     /translation="MIPGTAIPTSRPQVDDTLTPARASQKVSDSLSDFQPGQRLVATI
                     QSQLANGTYRAMIAQRDVTLALPFSAKAGDQFKLEVVDTRGRVAFALAEDAPPAEGKT
                     QQPASTTTTLSQAGRVIGTLFAKPTPGAPPGSTAEPAPTPLNRGQPLAPPPTENGAQL
                     APALQKAVTQSGLFFEAHLAAWLAGKHAKAALMQEPLALLPRPATADAAGRGGEAAVR
                     GGSGGAGGPPVAAGTGHGGAPAASSTAATSSPSAAVAMTASAPQNTASRGLQAPPPAA
                     LPGAAAPAGDASTARGATAAPLFNLPTEAAPVVHQQLNALATNVYAFQAQAWIDQRVS
                     WEIVDPEGHQGGAEEGPAKTWRSRLSLTMPALGTVEAMVYLRGDRLDIRFEVPRQDTR
                     QTLQDARPALTARLTDLNLRLESLRIDAPAADEPADA"
     gene            4399..4752
                     /locus_tag="VIS73_08350"
     CDS             4399..4752
                     /locus_tag="VIS73_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEY5763205.1"
                     /translation="MSRQTPEPPPERAIDLRQLAVALAYRDGDNAPRVVAKGRGLLAE
                     AIIARAQGAGVYVHESPELVSLLMQVDLDERIPPQLYQAVAELLAWLYRLDRGLQPED
                     GKAGRSPLGGPVAAR"
     gene            complement(4749..>5246)
                     /locus_tag="VIS73_08355"
     CDS             complement(4749..>5246)
                     /locus_tag="VIS73_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEY5763206.1"
                     /translation="EMKSRFAGDVSTEHLQLVTFPAGAVMIPNPFNRIAGFSLGDQHF
                     VPGFPQMAWPMIEWVLETRYAGWFNLSPRVERAIWAHGPNAYESYLLELMERVVRDYP
                     DLRLFSLPSMGEGGQRRHLEIGVEGAPARVEAAMAAIIAGVERRGVAWCEPGSMPPET
                     PSTSP"
BASE COUNT          961 a         1797 c         1641 g          847 t
ORIGIN      
        1 cacacagccg ggtcaaatcg cggcacgcct tcaaggcttt aactttttgg ccgataatga
       61 gttatccctt aggcactcgg accgctgacc catggcgata tcctctcccg gcattggctc
      121 ctcgctcgac gtcaacagca tcgtttcgca gctgatggca cttgagcagc gtccactgac
      181 cttgctcggc atcaaggagg ctcgccttca aagtcggctg tcggcgatcg gccaggtcaa
      241 gggcgcgctt tcgtccattc agtcggggct tgccaatctc aaggacgcga gccgtttcag
      301 cagcttcaag ggagccagtt cggacgccac ggtagcttcg gtgacggcca gcactttcgc
      361 tgaaccgggc acatacaacc tcgacgtcac ccagttggca agcgcccaga agctcaattc
      421 acaggctttc gccacggccg aaacggtggt cggcggtggc acgctcaccc tgcaattcgg
      481 cagtttcaat ggcgcaagct tcaccgccaa cagtgaaaag tcagcaatca acctgacgct
      541 cgcggccgat gccacgctgg caagcgtgcg ggatgcaatc aatggcgcca aggccggggt
      601 cacggccagc atcgtcaatg acgggaccgg atttcgcttg gcgatttcgt cgagccagac
      661 cggcgctgcg ttcggcctca agatcactgc atcggatctc gatggcaaca ataccgacga
      721 tgccgggcta tcgcggctgg ttttcgaccc agccggcacc aagcgcctga acgagtcaca
      781 agccgcacgc gatgcggcgt tcacgatcga tggaatcgcg atcagccagg ccggaaacac
      841 ggtgaccaat gcgctctcgg gcgtgacgtt gaacctcatc aaggtgggaa ccagcacgct
      901 gtcggtgtcg cgtgatacat cagcggtcaa gtccgccatc gacggtttcg tcaaggcata
      961 caacgacggc aacaaggttc tgacgcaatt gggcgctttc aatgcggaga cccgcgaagc
     1021 cggtgcgctg cagggtgact caacgctgcg cacgatccgc gggcagatgc gctctctgct
     1081 gggcggaaca ctgcccttcg cctccggcgg actgcgcaac ctgtcggaaa tcggcctgag
     1141 tttcgcccta gacggctcgc tgacgctcga cgccagcaag ttgcaggcgg ttctcgacga
     1201 cccgaccaag gacgttgcct ctctattcgc ctccgtcggc cgccccagcg acccgagcat
     1261 tcgcttcggt tcggcaacaa acgcggtcaa gcccggcgac tacgcgctgt cggtcacgca
     1321 gttggcgacc cgcggaacga tcacggcatc gtcgctgctc aatggcgtcg tcgctgccgg
     1381 catcaatgac agcgtgggcc tgaccgtcaa cggtagcgtc gtcaatgtct cgctttccgc
     1441 aggttcgtac agcgccgccg gcttcgcggc cgagttgcag tcacgcataa acggggcact
     1501 gagcgggact gcggcgtccg ttgcggtgac ggccaccgga acaggtggtt cactcgccgg
     1561 ctcggccgcc gcgcccctgc tgatcgacgc aaccaacaac acgatcgacg tcacgcttaa
     1621 cggggtcaca cacaccgtga ccctgacgca ggacacgtat ctttcggcca gtgcggtcgc
     1681 cgccgagatc caggcaaaga ccaacgctgc cttcgcggca gacggattcg ccgtcgccgt
     1741 caccgaagct ggaggcgtgc tgaccatcag cgccgccaac agtttcggca cgggctcatc
     1801 gatcgcggta aatggcctcg gcgcggatgc actgctcggc gtcgcccgca ccgccacggc
     1861 gggcgcggct ggcacactga gcatcacgtc aaccaactac ggatcaacct cgacgctggc
     1921 cggattgtcg gtcgatgcgt ccctggtgac acccggctcg atcgacaccg ccagcgctag
     1981 tggtgtcgat gcggcgggca ccctaggcgg cgccagcgca accggttccg ggcaggtgct
     2041 caacgccgcc ggactctcgg tagtcgtcac gggcggcgcc acgggcaacc gtggttcggt
     2101 ttcgttcgac cgcggcttcg cttcgcagct cgacaccttg ctgaccagcg cactttccag
     2161 caccgggctg ctggagtccc gcgtgcaagg catccagtcg tcgatcgatt caattggcga
     2221 taaccgagaa ttgatcgagc gacgcctggt gtcgatcgaa gcgcgcctac gccgccagtt
     2281 cacggcgctg gataccttga ttgccgggct gtcgcagacc agcaacttcc tccaacagca
     2341 gcttgcgacc ctgccgggcg ccagcagcgg caattcctga aaaattggag cgcgccatgt
     2401 tcggtaacta caacgcggta tcagcctaca agtcggtcgg cgtcgattcc gcggtcaccg
     2461 cagccgatcc gcacaagctg atcctgctac tgctcgacgg cgggctgtcg gccatatcga
     2521 tcgccaaggg cgccatggcg cagggtcagg tagccggaaa gggcgaagcg atttccaagg
     2581 caatcgatat cgttgagaac ggtcttcgcg ccagcctcga caaggagcaa ggcggagagt
     2641 tagcgcttca gctcgacctg ctttatgagt acatgggccg gcgcctgctt cacgccaacg
     2701 tcaagaacga ctctgccgcg ctcgacgagg tcagcgcgct gctgcgtgaa attcacgcag
     2761 catggtccga aattcgcaag cgggcgattg accaggccgc caatcccgcg gctgccgcct
     2821 aaccaacttc caatgaattc gggcgactgc tggagccaac tggcggattc ggcccagcgc
     2881 atggcgaatg ccgcacagtc gcacgactgg gacgctctga tcgatcagta cggacggttc
     2941 gccgcaatcg ctgaaaacct gccgcggcac cagcttccat tgccgtccga cgccgcgcgc
     3001 cgcgagcgtg agcagctgat tcggcgagtt ctggaagatg tcgaggcagt ccgcgaacag
     3061 gtcggtccct ggatgaccga cgtccggccg ctgctcgaag cttggcggcg ctcggaaaca
     3121 cccgccccat gattcccggc accgcgatcc cgaccagtcg gccacaggtc gacgacacgc
     3181 tgacgccggc gcgcgcctcg cagaaggtca gcgattcgct ctccgacttc cagcccggcc
     3241 agcgcctggt ggcgacgatc cagtcgcaac tcgccaacgg cacctaccgc gcgatgatcg
     3301 cccagcgcga cgtgaccctc gcgctgcctt tttcggccaa ggccggcgac cagttcaagc
     3361 tggaggtggt ggatacacgc ggccgggtcg cctttgcgct ggccgaggat gcaccgcccg
     3421 ccgagggcaa gacacagcaa ccggcctcga cgacgaccac cttgagccag gccggacgcg
     3481 tcatcggtac gctgtttgcc aagccaacgc ccggcgcgcc cccgggcagc acggccgagc
     3541 cggcgccgac accgctgaat cggggccagc cgctggcccc gcccccaacc gaaaatggcg
     3601 cccaactggc acccgccctg cagaaagccg tgacccagag cggacttttt ttcgaagcgc
     3661 acctcgccgc ctggcttgcc ggcaagcacg cgaaagcagc gctgatgcag gaacccctcg
     3721 cgctgctgcc acggcccgcg acggccgatg ccgcgggacg cggtggcgag gccgcggtcc
     3781 gcggtggcag tggcggagcc ggtgggcccc cggtagcggc tggcaccggc catggtggcg
     3841 cgcccgccgc ctcaagcaca gcagccactt cctctcccag cgccgcagtg gcgatgacgg
     3901 cgtcggctcc gcaaaacacc gccagtcgcg gattgcaggc accgcccccc gcagcgttgc
     3961 cgggggccgc cgcgcccgca ggagacgcgt cgaccgcacg cggcgccact gccgcaccgc
     4021 tgttcaacct gcccaccgaa gcagcaccgg ttgtgcacca gcagctcaac gcattggcga
     4081 ccaacgtcta tgcgttccag gcgcaagcct ggattgatca gcgagtcagt tgggaaatcg
     4141 tcgatccgga gggccatcaa ggcggtgccg aggaagggcc ggcgaagacc tggcgttcgc
     4201 gcttgtcgtt gaccatgccg gcgctcggaa cggtcgaggc gatggtctac ctgcgcggcg
     4261 atcgcctcga catccgcttt gaagtgccgc gccaggatac ccggcagaca ctccaggatg
     4321 cccgtccggc gttgactgcg cggctgaccg acctgaacct ccgactcgaa tcgctgcgca
     4381 tcgatgcgcc ggctgccgat gagccggcag acgcctgagc cgccgcccga gcgcgcaatc
     4441 gatctgcgcc aacttgcggt cgcgctcgcc tatcgcgacg gcgacaacgc cccgcgcgtc
     4501 gtcgccaaag gccgcggcct gctcgccgag gcaatcattg cccgggcaca aggcgcaggc
     4561 gtctacgtgc acgaatcgcc cgagctggtt tcactgttga tgcaggtcga cctcgacgag
     4621 cggattccgc cgcagcttta tcaggcggtc gccgaactgc tcgcctggct gtatcggctt
     4681 gaccgcggcc tgcagcctga agacggcaag gccggccggt cgccgctagg cgggccggtc
     4741 gccgcccgct aaggactggt agagggcgtt tccggcggca tcgagccggg ttcgcaccaa
     4801 gcgacgccac gccgctcgac accggcgatg atcgccgcca tggccgcctc gacgcgtgcc
     4861 ggcgcccctt cgacaccgat ctctagatgg cgacgctgcc cgccctcgcc catcgacggc
     4921 agcgagaaca gccgcaggtc cggatagtcg cggacgacac gttccataag ctcgagcaag
     4981 tagctttcat aggcgttcgg gccatgcgcc cagatcgccc gttcgacgcg tgggctcagg
     5041 ttgaaccagc ccgcgtagcg ggtctcgagc acccactcga tcattggcca ggccatctgc
     5101 ggaaaaccgg gcacgaagtg ctggtcgccg agcgagaagc cggcgatgcg attgaaggga
     5161 ttggggatca tcactgcgcc ggccggaaag gtcaccagtt gcaggtgctc ggtcgataca
     5221 tcgccggcga atctcgattt catctc
//
LOCUS       DATDAA010000264         3044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_299415,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000264 DATDAA010000000
VERSION     DATDAA010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299415"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /gene="cysE"
                     /locus_tag="VIS73_08360"
     CDS             complement(<1..672)
                     /gene="cysE"
                     /locus_tag="VIS73_08360"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEY5763207.1"
                     /translation="MVDRIAWNWPSRPKVKAWRAAQQERQGMFDRLKEDIASVFDRDP
                     AARSFAEVLFCYPGIHALLMHRLAHRLWIARWLWLARFIAHISRFLTGIEIHPGARIG
                     RKFFIDHGMGVVIGETAEIGDGVTLYHGVTLGGTSWNEGKRHPTLGDGVVVGAGAKVL
                     GPILIGNNAKVGSNAVVFKEVPAGATAVGNPARVIDDEQAKQREEKAEKMGFSAYAVA
                     ENMDDP"
     gene            complement(669..1472)
                     /locus_tag="VIS73_08365"
     CDS             complement(669..1472)
                     /locus_tag="VIS73_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY5763208.1"
                     /translation="MQDSGANPEGAHSTAKDPLAGVLSRVRVVLCRPRHPGNIGAAAR
                     AMKTMGLSRLVLVAPERYVAPDDEALARASGADDVLLAAQVVDSLDVALQGVVCAAAL
                     TARRREVEPPRMALREASSGLIEHATRGGEIALVFGGETAGLSNDEVMRCDLIVSLST
                     NPVYGSLNLGAAVQVLAHELRMAAAGAALADPRAVGERLSPLATRDEIEGLMRHLEAQ
                     MAATGFFDAGNPKRLMPKLRRLFGRSEMERDEVNILRGFLASIERFGRK"
     gene            1503..2303
                     /locus_tag="VIS73_08370"
     CDS             1503..2303
                     /locus_tag="VIS73_08370"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY5763209.1"
                     /translation="MHPALNIAIKAARRAGQIINRASADIDIVRVASKSTNDFVTEVD
                     HAAEAAIIEVLRDAYPKHGILAEESGESLADAEYQWIIDPLDGTTNFIHGFPQYAVSI
                     GLAHRGQMTQAVIYDPGRNDLFTASKGRGAFLNDRRLRVSKRFRLNEALIATGFPFRS
                     FEHIDTYLAIFKELAQKTAGIRRPGSAALDLAWLAAGRTDGFWEFGLAPWDIAAGTLI
                     VTEAGGLVADLAGEANYLKTGNLVAGNPKIFAQLLQILGQHRNTALSA"
     gene            complement(2395..>3044)
                     /locus_tag="VIS73_08375"
     CDS             complement(2395..>3044)
                     /locus_tag="VIS73_08375"
                     /inference="COORDINATES: protein motif:HMM:NF044548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HEY5763210.1"
                     /translation="KKHKPKSKILVLDANPDIASKGKLFRKGWEKYYQGLIEYKGAQK
                     VTKVDAASNTVWVEGIEAVKGDVVNLVPPQRAGAIAGKAGLIGPDGKWCPVNQQTFES
                     SLVPDIHVIGDACIAGAMPKSGYSANSEAKICALNIVALMNGRETIALSGINTCYSFL
                     TDDEAVSVAAVYTFDEEKKAIVSVKDSGGVSPDLSKLEATYAESWLRNILTEMSS"
BASE COUNT          542 a         1045 c          899 g          558 t
ORIGIN      
        1 tggatcgtcc atattttcgg caacggcgta ggccgaaaaa cccatcttct ccgccttctc
       61 ttcgcgctgc ttcgcctgtt cgtcgtcgat gacccgcgcc gggtttccga cggcggtcgc
      121 acccgccggc acttccttga acacgaccgc attggatcca accttggcgt tgttgccgat
      181 caggatcggg ccgaggacct tggcgccggc gccaacgacg acaccatcgc ccagggtcgg
      241 atgccgcttg ccttcgttcc agctggtgcc gccgagcgtg acgccgtggt agagcgtcac
      301 gccgtcgccg atctcggcag tctcgccgat gaccacgccc atgccgtggt cgatgaagaa
      361 cttgcggccg atgcgggcgc ccggatggat ttcgatgccg gtcaggaatc tcgagatgtg
      421 cgcgatgaac cgggccagcc agagccagcg tgcaatccac agccggtgcg cgaggcggtg
      481 catcaggagg gcatggatgc cgggatagca gaagagcacc tcggcaaatg agcgtgcggc
      541 cggatcccgg tcaaaaaccg aggcgatgtc ttccttcaag cgatcgaaca ttccctgtct
      601 ttcctgttga gccgcgcgcc aagctttcac cttggggcgc gacggccaat tccaagcaat
      661 tctgtcgact attttctgcc gaagcgttcg atcgaggcaa ggaagccgcg caagatattg
      721 acctcgtcgc gctccatctc cgacctgccg aacagtcgcc gcagcttggg catcaggcgc
      781 ttcggattgc cggcgtcgaa gaatccggtc gccgccatct gcgcctcaag atgcctcatc
      841 aggccctcga tttcgtcgcg cgtcgccagc ggcgacaagc gttcgccgac agcccgcggg
      901 tcggccagcg cggcgccagc cgcggccatg cgcagctcgt gtgcgagcac ctgcacggcc
      961 gcgcccaggt tgagcgagcc gtacaccggg ttcgtcgaca aactgacgat caggtcgcag
     1021 cgcatcacct cgtcgttgct caggccagcc gtctcgccac cgaacaccag cgcgatctcg
     1081 ccgccgcggg tcgcgtgttc gatcaggccg ctgctcgctt cgcgtagcgc cattcgcggc
     1141 ggctcgacct cacgccgcct ggcggtcagc gcggcggcac agaccacgcc ctgcagtgcc
     1201 acgtcaagcg aatcgacgac ctgtgccgcc agcagcacat cgtcggcgcc cgacgcgcgc
     1261 gccagcgctt cgtcgtcggg cgccacgtag cgttcgggcg ccaccagcac cagtcgcgac
     1321 agccccatcg tcttcatcgc ccgcgctgct gccccgatat ttccggggtg gcgcggccga
     1381 cagaggacga cgcggacgcg acttaaaacc ccggcaagcg gatccttcgc ggtagaatgc
     1441 gcgccttcgg gattggcgcc gctgtcctgc atggcgctcc cactgttctt ttccagaccc
     1501 atatgcatcc agctctcaac attgccatca aggcggcgcg acgcgccggc cagatcatca
     1561 accgcgcatc ggccgatatc gatatcgttc gcgtcgcctc aaagagcacc aatgacttcg
     1621 tcaccgaagt cgatcacgcc gccgaagcag cgatcatcga agtgctgcgc gatgcgtatc
     1681 cgaagcacgg gattctcgca gaagaatcgg gcgaatcgct cgctgacgcc gaataccagt
     1741 ggatcatcga cccactcgac ggcacaacca acttcatcca cggcttcccg caatatgcgg
     1801 tgtcgatcgg cctcgcccat cgcgggcaga tgacccaggc ggttatctac gacccgggcc
     1861 gcaatgatct gttcacggcg agcaagggcc gcggcgcttt cctcaacgat cgccgcctgc
     1921 gcgtctcgaa acgctttcgc ctcaacgagg cgctgatcgc caccggcttt ccgtttcgca
     1981 gtttcgaaca catcgacacc tacctggcga tcttcaagga actcgcgcag aagaccgccg
     2041 gaatccgccg accgggctcc gccgcgctcg acctggcctg gcttgctgcc ggtcgcactg
     2101 acggcttctg ggagttcggt ctggcaccct gggatatcgc cgccggaacg ctgatcgtca
     2161 ccgaagcggg cggcctcgtc gccgaccttg ccggtgaagc gaactacctg aagaccggca
     2221 atctggtcgc cggcaatccg aagatattcg cgcagcttct gcagatcctc ggacagcacc
     2281 gcaacaccgc gctttccgcc tgaaccgcgt caccagaaga aatccccgtg cgttgccaca
     2341 cggggattgt cctgaacgaa ggaggcgcga gcccccttcg cgactgaccg gcgatcaact
     2401 cgacatttcg gtcaggatgt tgcgcaacca gctctcggcg taggtcgcct caagcttcga
     2461 caggtcgggc gatacgccac ccgaatcctt gaccgagacg atcgccttct tctcttcgtc
     2521 gaaggtatag accgccgcga cgctgaccgc ctcgtcgtcg gtaaggaagc tgtaacaggt
     2581 attgattcct gacagcgcga tcgtttcacg gccgttcatc agcgcgacga tgttcagcgc
     2641 gcagatcttc gcttcggagt tggccgagta gcccgacttc ggcatggcgc cggcgataca
     2701 ggcgtcaccg atgacgtgga tgtcaggaac cagcgaagac tcgaaagtct gttggttgac
     2761 cggacaccac ttgccgtcgg gaccgatgag gccagccttg ccggcaatcg caccggcacg
     2821 ctgcggcggc accaggttga ccacgtcgcc cttgaccgcc tcgatgcctt cgacccagac
     2881 cgtgttgctg gccgcgtcga ccttggtcac cttctgcgca cccttgtact cgatcaggcc
     2941 ctggtagtac ttctcccagc ccttgcggaa caacttgccc ttggaggcga tgtccgggtt
     3001 ggcgtcgagc acgaggatct tcgacttcgg cttgtgcttc ttca
//
LOCUS       DATDAA010000265         5755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_124451,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000265 DATDAA010000000
VERSION     DATDAA010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124451"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..955)
                     /gene="motA"
                     /locus_tag="VIS73_08380"
     CDS             complement(95..955)
                     /gene="motA"
                     /locus_tag="VIS73_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEY5763211.1"
                     /translation="MALIVGYIVVIASVLGGFALAGGHLGSLLQPVELIMIGGSAAGA
                     FFIANPPKVIKAVFKSIPTLFKGSRHSKARYVDLLALLYEILAKVRKEGLMSIESDVD
                     SPEQSPLFSKYPTIVSDHHLVEFTTDYLRMMVGGNLNAFEIENLMDNEIETHHHEAAL
                     PSGALAKLADGLPAFGIVAAVMGVVHTMESVGIPPAELGKLIAAALVGTFLGILLSYG
                     FVGPLATLLEHKADEEAKQYQCVKVVLLASMSGYAPQVAIEFGRKVLYASERPSFIEL
                     EQEIKERKSR"
     gene            1093..1671
                     /locus_tag="VIS73_08385"
     CDS             1093..1671
                     /locus_tag="VIS73_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY5763212.1"
                     /translation="MRYCTACGGELLLQVPAGDTLPRHVCGRCGEIHYQNPKMVVGCV
                     ATHDEHVLLCRRAIEPRHGLWTLPAGFMENGETSAAAAARETHEEACAVVEVGELFSL
                     VDVPHISQVHLFYLGRLHSDQHAAGIESLETGLFVEADIPWDELAFRSVSFTLRAFFA
                     DRRCGRFGVHTQVLAPQASIAPVVQCVADRKT"
     gene            complement(1627..2385)
                     /locus_tag="VIS73_08390"
     CDS             complement(1627..2385)
                     /locus_tag="VIS73_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY5763213.1"
                     /translation="MIPTAQAAPVRGRVAGLLRSAIAPAFLVSGLLHAVALSWGLAPG
                     HSAAPAGLGALPLQVRLAEPTPAPVASPTRPALAAAPRQSTAPPAAAVMAVPAPEVRR
                     LAVPAVSEAAARPVVAPPVATALDDSPRREPLPAVVSTAASRIADAQAESDTVRADYQ
                     ALLRAAAERLQRYPERARRLGFEGTAVVAVELQPDAAALRVVLVRSSGIGMLDRAALD
                     LLDRAAREVDPATASVGQGQLRFPVRYALHDGRD"
     gene            2414..3028
                     /locus_tag="VIS73_08395"
     CDS             2414..3028
                     /locus_tag="VIS73_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY5763214.1"
                     /translation="MNAPADLFTLAELSSRPLILALDAHGIPSRWISWQQACFYYARG
                     MIAWVAGDMSLIIRGGHSRITGEQSLITANSIIALRGAAHAASQLHRTPPLNNRELFH
                     RDRHICAYCGNEYGTVRLTRDHVLPLSQGGGDTWMNLVTACRGCNQRKGGRTPERAGM
                     QLLYAPYIPNRAEYLILSNRHILADQMDFLARHVPAQSRVRLAS"
     gene            3042..3824
                     /locus_tag="VIS73_08400"
     CDS             3042..3824
                     /locus_tag="VIS73_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEY5763215.1"
                     /translation="MNQTTGEPTPDEADLVRRFTGTRRLYGDVAADTFAAAHVCVIGI
                     GGVGSWVAEALARSAVGALTLVDLDMVAESNVNRQIHAVSGAFGAAKVEVMARRIAGI
                     NPACVMTTIEDFATADNAASLLGRGFSVIVDAIDDAPAKVALIAAARRLSLPLVTVGG
                     AGGRRDPTRIRCDDLARSAHDPLLARVRRDLRKQHHFPAKGEFGIRAVFSQEPMSVPP
                     EDACDPSGRLACSGYGSAVAVTGSFGFAAAAEALRLIGERAG"
     gene            complement(3825..4784)
                     /locus_tag="VIS73_08405"
     CDS             complement(3825..4784)
                     /locus_tag="VIS73_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5763216.1"
                     /translation="MQTLISYTDGIHAVDAMYVRPRLASIHLIVEKGRAAFFDTGTTH
                     SLPQVLAALDSLGLTPESVDYVIPSHVHLDHAGGSGAMMRAFPKATLIVHPRGARHLV
                     DPAKLVAGTVAVYGQQRTDELYGEIVPVDPARLVQASDGMVVDLAGRPLTLIDTPGHA
                     KHHLCLHDARTNAIFTGDMFGLSYRELDVAGKPFVFATTTPVHFDPPAMRRSLDRLLA
                     LKPKTAYLTHFSRVGAHVDDLERLAADLWRLVEAHVEIARFANWGEAADRHARIVKAL
                     WQLLLEEKARQGWTLDDAALRALFDDDLELNAQGLEVWLDTTV"
     gene            4897..5751
                     /locus_tag="VIS73_08410"
     CDS             4897..5751
                     /locus_tag="VIS73_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5763217.1"
                     /translation="MSHFPADDGVRIHVAVDSASAGGLPVVMLHGWTASADEWSPFLP
                     AITAHHPVYRWDARGHGRNNPEAATSAAAAHPTVQRMARDLDNLLSHFALDRVVAVGH
                     SMGALTLWQYIRDFGTRRLAKVCLIDQSPRLVTDGEWTHGIYGNFDPARSRAFVDDLR
                     ADFPEAVLRLTALGLNARAREKYLDNARGWQRSREMLARLDPDPLIACWESLVGADYR
                     DVLAAIDVPALLVYGGESNFYHAETAHYVASKIPDAILNIYEGTDHSPHQWQRERFAR
                     ELVDFINT"
BASE COUNT          976 a         1983 c         1861 g          935 t
ORIGIN      
        1 cgccaccgtg atggccatgc gcggccttct tgatgcgctt gacgattatc gggcgctggg
       61 aatcgtcact catgatgcat cagcccgtcg gcgctcagcg cgacttgcgt tccttgattt
      121 cctgctccag ctcgataaag ctcgggcgct cgctcgcata gagcaccttg cggccgaatt
      181 cgatggcaac ctgcggcgcg taacccgaca tcgacgccag cagcacgacc ttgacgcatt
      241 ggtactgctt ggcttcctcg tcggccttgt gctcgagcag cgtcgccagc gggccgacga
      301 agccgtagga aagcaggatg ccgaggaagg tgccgaccag cgctgcggcg atcagcttgc
      361 cgagttcggc cggcggaatg ccgaccgact ccatggtgtg cacgacgccc atcaccgccg
      421 cgacgatacc gaaggcgggc aggccatcgg caagcttggc gagcgctccc gacggcaagg
      481 ccgcttcgtg gtggtgcgtt tcgatctcgt tgtccatgag gttttcgatc tcgaaggcat
      541 tgaggttgcc gccgaccatc atgcgcaggt agtcggtagt gaactcgacc aagtggtgat
      601 ccgaaacgat ggtcggatat ttcgagaaca gcgggctttg ctcgggcgag tcgacgtcgg
      661 attcgatcga catcaggcct tccttgcgca ccttggcgag aatctcgtag agcaacgcca
      721 gcaagtcaac gtagcgcgcc ttgctgtggc gcgaaccctt gaacagcgtc ggaatcgatt
      781 tgaagaccgc cttgatgacc ttgggcggat tggcgatgaa gaaggcgccc gccgccgagc
      841 cgccgatcat gatcagctcg accggctgca gcagcgagcc gaggtggccg ccagcgagcg
      901 caaaaccccc cagcaccgag gcgatgacaa cgatgtagcc gacaatcagc gccatgcgtg
      961 cgccacccct cccctgcatg ttgtccagca gcgcggcgca tcccgcccgc ggcgattcgc
     1021 gattatcatc gcccgacgcc cgcatggcaa tgccgggtct gacaacgcga cgagttgaaa
     1081 ccgatgatcg tgatgcgcta ttgcactgcc tgtggcggcg agttgctgct gcaagttccg
     1141 gcaggggaca cgctgccgcg gcacgtctgt ggacgctgcg gcgagatcca ctaccagaac
     1201 ccgaaaatgg tcgtcggctg cgtcgccacg cacgacgagc acgtcctgct gtgccgccgc
     1261 gcgatcgaac cgcgccacgg cttgtggacg ctgcccgctg gcttcatgga gaacggcgaa
     1321 acgagcgccg cggcggcggc gcgcgaaacc cacgaggaag cctgcgcggt ggtcgaggtc
     1381 ggcgagctgt tcagccttgt cgacgtgccg cacatcagcc aggtgcatct gttctatctc
     1441 ggccgcctgc actccgacca gcacgcagcc ggcatcgaga gtctggaaac cgggttgttt
     1501 gtcgaagcag acatcccctg ggacgagctc gccttccgca gcgtcagctt tacgctgcgc
     1561 gcattcttcg ctgaccggcg atgcggccgc ttcggtgtgc acacgcaggt gctcgctccg
     1621 caggcctcaa tcgcgcccgt cgtgcagtgc gtagcggacc ggaaaacgta gctgcccctg
     1681 gccgaccgac gctgttgccg ggtcgacctc gcgcgcggca cggtcgagca gatcgagcgc
     1741 ggcacggtcg agcatgccga tgccgctcga gcgcaccagc accacgcgca gcgcggcggc
     1801 gtctggctgc aactcgaccg cgaccacggc cgtcccctcg aaacccagtc gccgcgcccg
     1861 ctcgggatag cgctgcagcc gctcggcagc ggcgcgcaac agtgcctggt aatcggcccg
     1921 gacggtatcg gattcagcct gagcgtcggc aatgcgggac gcagcggtgc tgaccacggc
     1981 cggtaacggc tcgcggcgtg gcgaatcgtc gagcgcagtg gctactggcg gggcaacaac
     2041 cggccgtgca gccgcttcgc tgaccgctgg taccgccagc cggcgtactt ccggcgccgg
     2101 taccgccatg actgcagcgg ccggtggcgc cgttgattgg cgcggcgcgg cggccagggc
     2161 cggtcgcgtc ggcgaggcga cgggggccgg tgtcggctcg gctaggcgaa cctgcaacgg
     2221 cagcgcgccc aagccggcag gcgccgcgga atggcccggc gccagacccc acgacagggc
     2281 cactgcgtgc aggaggcccg agaccagaaa ggccggcgca attgccgaac gcagcagccc
     2341 ggcgacgcgc ccgcgaacag gcgctgcctg ggccgttgga atcattgcgc cgctttgata
     2401 ggctacgcgc accgtgaacg ctcctgccga tttatttacg ctggccgaac tctcatcccg
     2461 cccgctcatt ctggcgctgg acgcgcacgg catcccgagc cgatggatca gttggcagca
     2521 ggcctgcttc tactacgccc gcggcatgat cgcctgggtc gccggcgaca tgtcgttgat
     2581 catccgcggc ggccactccc ggatcaccgg cgaacaatcg ttaataaccg ctaacagcat
     2641 catcgccctg cgcggcgctg cacacgctgc cagccagctg catcgcacgc caccgctcaa
     2701 caaccgcgaa ctgttccatc gcgaccggca catctgcgcc tactgcggca acgagtacgg
     2761 caccgtgcgc ctgacccgcg atcacgtcct gccactgtcg caaggtggcg gcgacacctg
     2821 gatgaacctg gtgacggcct gccgcggctg caatcagcgc aagggcggcc gcacgcccga
     2881 gcgtgccggc atgcaactac tctacgcacc ctacataccc aatcgcgccg agtacctgat
     2941 tctatcgaat cggcacatcc tcgccgacca gatggacttt cttgcccgcc acgttccagc
     3001 gcagagccga gtccggctcg cttcctgaag gccactcccg catgaaccag acgaccggcg
     3061 aaccgacgcc ggacgaggcc gacctcgtcc gccgctttac cggcacccgc cgcctctacg
     3121 gagacgttgc cgccgatacc ttcgccgcgg cccatgtctg cgtcatcggc atcggcggtg
     3181 tcggctcctg ggttgccgag gcactggccc gctcggcggt cggcgcgctg acgctggtcg
     3241 atctcgacat ggttgcggag tccaatgtaa accggcagat tcacgcggta tccggcgcct
     3301 tcggggcggc caaggtcgag gtgatggcgc ggcgcatcgc cggcatcaac ccggcctgcg
     3361 tgatgacaac gattgaggat ttcgcgactg ccgacaacgc tgcgtcgctg cttggacgcg
     3421 gcttctcggt gatcgtcgac gcgatcgacg acgcgccggc gaaggtggcg ctgattgccg
     3481 ctgcccgccg gctttcgctg ccgctggtga ccgtcggcgg tgccggcggc cggcgcgatc
     3541 cgacgcggat tcgttgcgac gacctggcgc gcagcgcgca cgatccgctg ctcgcgcggg
     3601 tccgccgtga cctgcgcaag cagcaccatt ttccggcgaa aggggagttc ggcatccgcg
     3661 ccgtgttctc gcaggaaccg atgagtgttc cgcctgaaga cgcttgcgac ccgagcgggc
     3721 gactggcctg cagcggctac ggctcggccg tcgcggtcac cggcagcttc ggttttgccg
     3781 cggcggccga agcactgcgc ctcattggcg agcgcgcagg ctaatcagac cgtcgtatcg
     3841 agccagacct ccaggccctg cgcgttcaac tccaggtcgt cgtcgaacag cgcacgcagc
     3901 gccgcatcgt ccagcgtcca tccctgtcgg gccttttcct caagcaacag ctgccacagg
     3961 gccttaacga tgcgagcgtg gcgatcagcc gcttcgcccc agttggcaaa gcgcgcgatc
     4021 tcgacgtgcg cctcaaccag gcgccataga tcggcggcca gccgctccag atcgtccacg
     4081 tgcgcgccga cacgcgagaa gtgcgtcagg taggccgttt ttggcttaag cgccagcagg
     4141 cggtctagcg agcgacgcat cgccggcgga tcgaaatgca cgggcgtcgt cgtcgcaaag
     4201 acgaagggct tgccggccac gtcgagctcg cgatacgaga ggccaaacat gtcgccggtg
     4261 aagatggcgt tggtacgcgc atcgtgcaga cacaggtggt gcttagcgtg ccccggcgta
     4321 tcgatcaatg tcagcggccg gccggcgagg tcgacgacca tgccgtcgct ggcttggacc
     4381 aagcgcgccg gatcgacggg aacgatctcg ccatacagct cgtcggtacg ctgctggccg
     4441 tacacggcga ctgtaccggc aaccagcttg gccggatcga cgaggtgccg tgcgccgcgc
     4501 gggtgaacga tcagcgtcgc cttcgggaag gcgcgcatca tcgcgcccga gccgccggcg
     4561 tgatcgagat gcacgtgcga agggatgacg tagtcaacgc tctcgggcgt cagcccgagg
     4621 ctgtcgagcg ccgccagtac ctgcggcagc gaatgtgtcg tgccggtatc gaaaaaggcc
     4681 gcgcggccct tctcgacgat caggtgaatc gacgccaggc gcgggcgaac gtacatcgcg
     4741 tcgacggcat gaatgccgtc ggtataggag atcagagtct gcatcgaagc ttccgtcgtt
     4801 ggtcgggcgg gaatggccgc gacccggcgc gttatcatcg ctcgcgcttc ttcgggtggc
     4861 acagccgcgg ccgcccattt tgccggaatt cccgcgatga gccacttccc tgccgatgac
     4921 ggcgtccgca tccacgttgc cgtcgacagc gcatctgcag gcggcctgcc ggtggtgatg
     4981 ctgcacggat ggacggcgag cgccgacgaa tggtcgccat tcctgccggc gatcaccgcc
     5041 caccaccccg tataccgctg ggacgcgcgc ggccacggtc gcaacaatcc ggaagcagcc
     5101 acctccgcgg cggccgcgca tccgaccgtg cagcgcatgg cccgcgatct cgacaacctg
     5161 ctgtcgcact tcgcccttga tcgcgtcgtc gccgtcggcc actctatggg cgcgctgacg
     5221 ctctggcagt acatccgcga cttcggcacg cgccgcctcg ccaaggtctg cctgatcgat
     5281 cagtcgccgc gactggtcac cgacggcgag tggacccatg gcatctacgg caacttcgac
     5341 ccggcgcgtt cgcgcgcctt tgtcgacgac ctgcgcgcgg actttcccga agcagtgctg
     5401 cggctgaccg cgctcggcct caacgcccgc gcccgcgaga agtacctcga caacgcgcgc
     5461 ggctggcaac gttcacgcga aatgctcgcg cgcctcgacc cggacccgct gatcgcctgc
     5521 tgggaaagcc tggtcggcgc cgactatcgc gatgtccttg ctgccatcga cgtgccggcg
     5581 ctgctggtct acggcggcga gagcaacttc taccacgccg aaaccgcgca ctacgtcgcc
     5641 tcgaaaatac ccgacgcgat cctcaacatc tacgaaggca cggaccactc gccgcaccag
     5701 tggcagcgcg aacgctttgc gcgcgaactc gtggatttca tcaacaccta ggtcc
//
LOCUS       DATDAA010000266         8781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_89002,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000266 DATDAA010000000
VERSION     DATDAA010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89002"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VIS73_08415"
     CDS             complement(<1..359)
                     /locus_tag="VIS73_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEY5763218.1"
                     /translation="MRKFLRRYLPGHEAIRDSRWLRPFSGSLLHPELWHLTRRSAAGA
                     VATGLACGLIPGPFQMLGAALVALIFRVNLPLALFVTLYTNPLTIVPLYLLAFEIGRW
                     TLGATDARFVAPPELVWP"
     gene            704..1594
                     /locus_tag="VIS73_08420"
     CDS             704..1594
                     /locus_tag="VIS73_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5763219.1"
                     /translation="MMGWWRRLRGAWVEREVVCASAAGLHRMAYVEWGNPKSDEVVIC
                     VHGLTRNGRDFDELARALSSRYRVVCPDIVGRGRSDWLKDPTHYGFPQYVADVTTLIA
                     RLDVTHVHWVGTSMGGLIGMFLASLDGNPIKSLVLNDVGPVIEGRALARIGEYVGQAL
                     VFADYAEAEAHIRTVAAPFGELTDRQWRHLTEHSIRRRDDDTWEMRYDPAIGKVFRML
                     APTQNIELWEIFDRIACPLLVTRGAESDLITHETAAKMVARHGVSGELAEFAGIGHAP
                     PFMNREQIDTVRDFIRRASH"
     gene            complement(1610..2383)
                     /locus_tag="VIS73_08425"
     CDS             complement(1610..2383)
                     /locus_tag="VIS73_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEY5763220.1"
                     /translation="MMRKLFGWLSPHKPQPLPEALWQATIATLPFLRGLSPDEIARLK
                     TLAEQFLAEKEFSAAEPLVLSDAVCLSIAVQGCLPILNLGLGWYRGWVGIVVYADEFV
                     IPREIQDEDGVVHRYDEVASGEAWEGGPLLVSWSDAQLAGEGYNVVIHEFAHKLDMLN
                     GEIDGIPPLHPGMDENVWSRVLLDAYADLCDRVDAGEDCAMDPYAAEDPTEFFAVASE
                     AFFETPDLLNADYPLLYAQLASFYRQDPLSRMAPRGLLE"
     gene            complement(2380..3285)
                     /locus_tag="VIS73_08430"
     CDS             complement(2380..3285)
                     /locus_tag="VIS73_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5763221.1"
                     /translation="MRWRFDHPVLLLSLTTLFWAGNWIVGRAFRDDVPPIALSFFRWN
                     VALACVLPLAWEELKTHEALVRRHWKILFFLGALGTALYNALTYIGLQTTTATNGVLL
                     NSFIPIVIVAINWLALRRQPSAIAIVGIAISFVGVMAIVARGSFANLTTLAFNVGDIW
                     IIASVFGWAIYTICLRLRPAELPPMATLAVLAAAGLVVMAPAFLFEWLVLGRTTNPTG
                     AAMAGILYTGIFPAFLGYIFWNRAVAEIGSEQAGLYIHLMPVFGIVLSSVFLGEHPQG
                     FHFAGIALILAGIALTSRRPVAIQP"
     gene            3410..5629
                     /locus_tag="VIS73_08435"
     CDS             3410..5629
                     /locus_tag="VIS73_08435"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY5763222.1"
                     /translation="MVSVVHSVGHSADDDISLARIAEGLSAVDAERVGSAARFARDIY
                     EERLLGSGESVWAHALGTALIAAGIKLDVEGRCAALLFAVPAYVEDAVPKLASRFGEA
                     IAQLVGGLSRLDHLRPIARGFVADADSSAEEKRAQIEILRKMLLAMVEDIRVVLLRLA
                     SRTQTLRHYARHEDELRVQVARETLDIYSPLANRLGIWELKWELEDLAFRYLYPDTYK
                     TIASQLDEKRIEREQFIATAIETLHRELAAMGIKAEIQGRPKHIYSIWNKMRRKDVPF
                     DEVYDVRALRVLVDEVKDCYSVLGLVHNLWAPISKEFDDYISRPKGNNYRSLHTAVHC
                     DDGRSLEIQIRTHEMHRHAELGVAAHWRYKEGGARGDGDRYEDKIAWLRQLLSWRDEV
                     AGSSDWVQQYKQAALHETIYVLTPQGRVIDLPLDATPVDFAYRLHTDLGHRCRGAKVD
                     GVLVPLNTPLKTAQRVEIVAAKQGGPSRDWLNPDLGYLTSKSARGKVRQWFASLQHDE
                     TVAEGRATVGKELQRAGLTSVNHEQLAARLHFAKADDLYAAMARGDVSGRQLAAAARG
                     EEAQDAAPGAAPVGSRQTRASDDAQSDILIEGVGKVLTQLARCCKPAPPDPIGGFITR
                     GKGVSIHRADCASFAHLAAMHPERVLDTEWGGKTRGVFGVDILIDADDRQGLLRDVSD
                     VLARERVNVTAVKTQSKQARATMRFTAEIENLDRLQVVLKQLQGVAGVVSARRAGGS"
     gene            complement(5626..8166)
                     /locus_tag="VIS73_08440"
     CDS             complement(5626..8166)
                     /locus_tag="VIS73_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5763223.1"
                     /translation="MPAKLSHASRVAPLTGGHRFSLTRYFAVTSLVCILMIAAALGWI
                     YQHLAMRELSRQAEIHNAELGQTLGNALWHRFDPLVARSPRLDSETILREIAERRLRE
                     DVITLMSGSKVIKVKLYNLDGLTVFSTDLTQVGALAAGNPGVKSALAGSVRSDLTHRN
                     TFDGFEGTLQNLDVLATYRPAIDSTGTIRGVFEIYTDVSDFVAATREVRSLVIGAVLA
                     LLGILYVLLHLLITRAQHILDQQSGELDATIDTIAKNNRELDQRVTDRTAELLAANQT
                     LQREVADRVAVEGKLRLAAQVFENSVEGITITDPEQTILAVNRAFTVVTGFSEAEAIG
                     QTPRLLKSGLQSPSFYKSMWKSLESTGHWQGEILNRRKNGEIYPEWLSISVVKDSFGN
                     VSNYVGVFSDISSLRDSEERIEYLSHHDAVTGLPNRLHFNQHVVMAIDAAVRQPTQFA
                     LVFLDLDHFKTINDSLGHDTGDAFLSEIATRLRRGVRSGDTLARLGGDEFITLVENID
                     DANDAALVAQYMLDTLEAPFTVEGHELLISASIGISLFPIDGSDVHTLVKHAEAAMYR
                     AKDVGRNAVRFYTPEMTANASERLQTEMQLRRALDNGEMTLQYQPQVELATGALVGAE
                     ALVRWTHPERGLVPPPKFIPITEETGLINDLGAWAFREACEQLQRWDAEGFHLPRLAV
                     NVSVRQIEREGFIDIVADILRDTGVSADRIEIEITESILMQTGKAPHLLAMLRELGVS
                     LSIDDFGTGYSSLSYLRRLPINKLKIDQSFIDDVTRNASDAAIVRSVIALATTMSLTT
                     VAEGVENEQQVEFLKREGCSVAQGYFFSPPMAPNELAAWAAQQRSANA"
     gene            complement(8200..>8781)
                     /locus_tag="VIS73_08445"
     CDS             complement(8200..>8781)
                     /locus_tag="VIS73_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEY5763224.1"
                     /translation="TGLGRAQKLHHLWQAPFSLHPNIIKAIERETANAQAGKPARIMA
                     KMNSLLEPKVIDALYVASQAGVKIDLLVRGACALRPGVAGLSENIRVRSIIGRFLEHH
                     RIFCFHNEGEDNVYLSSADWMERNFFRRIELCFPILDPRLKKRVLAEGLRVYLRDNHQ
                     AWELLADGRYEPRRGRGEPICAQYDLMKLLGDR"
BASE COUNT         1569 a         2658 c         3033 g         1521 t
ORIGIN      
        1 ggccagacca gttcgggcgg tgcgacgaat cgggcgtcgg tggcaccgag tgtccagcgg
       61 ccgatttcga aggcgagcag gtagagcgga acgatcgtca gcggattggt gtagagcgtg
      121 acgaacagcg ccagcggcag attgacccgg aagatcagcg ccaccagtgc ggcaccgagc
      181 atctggaagg gtccgggtat gaggccgcag gcgagcccgg tggcgacggc gccggccgcg
      241 ctgcgccgcg taagatgcca gagttccggg tgcagcaggc tcccggaaaa tggccgcaac
      301 caacgactgt cgcggatggc ttcatgcccg ggcaaataac gtcgcaggaa cttgcgcatc
      361 gcaccttctc gaatggcggg attctagccg agcgcccggt cgcctcacgc tggtcgggcg
      421 cgatactgtt cgcgtgctcg ccggaaccgg cgccgcagta ctgctcgttg ccggactgat
      481 cttacgcggc catcaactcg cgcccacact gctgatcgcc gcctcgttcg ccggaggctc
      541 tgcgtggtgc ggcgagcggg ccagtctggc gaaccgatcg cgttggtacg gtgagcgtgc
      601 atctcggtgc acaactcgcg gtgacgggcc tgtgtggcga acggccgagg tactggcgag
      661 gcagtgatac catcgccggc cttcgagcag taggagaagc ggaatgatgg gttggtggcg
      721 tcgattgcgc ggagcctggg tcgagcgcga ggtggtttgc gcgagtgcgg ccggcttgca
      781 ccggatggcc tacgtcgaat ggggcaaccc gaagagcgac gaggtcgtca tctgtgtcca
      841 cgggctgaca cgcaacggac gcgatttcga cgagctggcg cgcgcgctgt cctcgcgtta
      901 tcgcgtggtc tgtcctgaca tcgtcggtcg cgggcgcagc gactggctga aggatccgac
      961 acactatggg ttcccgcagt acgttgccga tgtgacgacg ctgatcgcgc ggcttgatgt
     1021 gacgcacgtg cactgggtcg gcacctcgat gggcggcctg atcggcatgt tcctcgcgag
     1081 cctcgacggc aatccgatta agagcctggt gctcaacgac gtcgggccgg tgatcgaggg
     1141 cagggcgctg gcacggatcg gcgagtacgt cggccaggcg ctggtgttcg ccgactacgc
     1201 cgaggccgag gcgcacatcc gcacggttgc cgctccgttt ggcgaactga ccgatcggca
     1261 atggcggcac ctgacagagc acagcattcg acggcgcgac gacgacacgt gggagatgcg
     1321 ctacgaccca gccatcggca aggtcttccg gatgctggcg ccgacgcaga acatcgaact
     1381 ctgggaaatc ttcgaccgca ttgcctgccc gttgctggtg acgcgcggcg ccgagtcgga
     1441 cctgatcacg cacgaaacag cggcgaagat ggtggcgcgg catggtgtca gcggcgagct
     1501 ggcggagttc gccggcatcg gccatgcgcc accgttcatg aatcgtgagc agatcgacac
     1561 tgtacgcgac ttcattcgcc gcgcctcgca ctgacctggc gacgcggcgc tattcgagca
     1621 gaccgcgcgg tgccatgcgg ctcagcgggt cctgccggta gaagctggcg agctgcgcgt
     1681 agagcagcgg atagtcggca ttgagcaggt ctggcgtttc aaagaacgct tcgctggcca
     1741 ccgcaaagaa ctcggtcggg tcctcggcgg cgtaggggtc catcgcgcag tcttcaccgg
     1801 catcaacgcg atcgcagagg tcggcgtagg cgtcgagcag cacgcggctc cagacgtttt
     1861 cgtccatgcc cggatgcagc ggcgggatgc cgtcgatctc gccattaagc atgtcgagct
     1921 tgtgcgcgaa ctcgtggatg accacgttgt agccctcgcc ggcgagctgc gcatcggacc
     1981 acgagacgag cagcggcccg ccctcccagg cctcgcccga ggcaacctcg tcgtagcggt
     2041 ggacgacgcc gtcctcgtcc tggatttcgc gcggaatcac gaactcgtcg gcgtagacga
     2101 cgatgccgac ccagccgcga taccagccca gcccgaggtt aaggatcggc aggcagccct
     2161 gtacggcgat cgacaggcag accgcgtcgc tcagcacaag cggctcggca gcgctgaatt
     2221 ccttttcggc gaggaactgc tcggccagcg tcttcaaccg cgcgatctcg tcaggcgaca
     2281 gcccacgcag gaacggcaag gtcgcaatcg ttgcttgcca gagtgcctcg ggtagcggct
     2341 gcggtttgtg cggcgagagc cagccgaaga gcttgcgcat catggctgta tcgccaccgg
     2401 gcggcggctg gtgagggcga tcccggcgag gatcaacgcg atgccggcaa agtggaagcc
     2461 ctgcgggtgt tcgccgagga agaccgagga gaggacgatg ccgaacaccg gcatcagatg
     2521 gatgtagagg ccggcctgct cgctgccgat ctcggccacg gcgcgattcc agaagatgta
     2581 gccgagaaag gcgggaaaga tgccggtgta gaggatgccg gccatcgccg cgccggtcgg
     2641 attggtagtg cgcccaagaa ccagccattc gaagaggaag gccggcgcca tgaccaccag
     2701 cccggcggcc gcgagtacgg cgagcgttgc catcggcggc agctcggccg ggcgcaggcg
     2761 caggcatatc gtgtagatcg cccagccgaa caccgaggcg atgatccata tgtcgccgac
     2821 gttgaaggcc agcgtggtca ggttggcgaa ggagccgcgc gcgacgatcg ccatgacgcc
     2881 gacgaaggaa atggcgattc cgacgatggc aatcgccgag ggctggcgac gcagcgccag
     2941 ccagttgatg gcgacgatca cgatcgggat gaacgagttg agcagaacgc cgttggtggc
     3001 cgtggtggtt tgcaggccga tgtaggtcag cgcgttgtag agcgccgtgc cgagggcgcc
     3061 gaggaagaac aggatcttcc agtgccggcg caccagcgcc tcatgcgtct tcagctcttc
     3121 ccaggcgagc ggcaggacgc aggccagcgc gacgttccag cggaagaagg acagcgcgat
     3181 cggcggcacg tcgtcgcgaa aggcgcgacc gacgatccag ttgccggccc agaacagggt
     3241 cgtcagcgag agcagcagta ccgggtgatc gaagcgccag cgcacggtgg attccctgca
     3301 ggagaagccg cgattatgcc gcgttgcgcc agcccagggg tggctctgaa agcgcgtcga
     3361 gcgcggcgtt caggcaacct taagccggca ctcatataat cgcgacatca tggtttcagt
     3421 cgttcactcg gtcggccata gcgccgacga tgacatctcc ctcgcccgca ttgccgaagg
     3481 cctgagcgcc gtcgacgccg aacgtgtcgg ctccgcggcg cgcttcgcac gcgacatcta
     3541 cgaggagcgc ctgctcggca gcggcgaatc ggtctgggcg cacgcgctgg ggaccgcgct
     3601 gatcgctgcc ggcatcaagc tcgatgtcga agggcgctgc gccgcactgc tgttcgcggt
     3661 gccggcctac gtcgaggatg cggtgcccaa gctcgcgagt cgttttgggg aggcgatcgc
     3721 gcagctggtt ggcggactgt cgcgcctcga ccacctgcgg ccgatcgccc gtggctttgt
     3781 cgccgacgcc gattcgagcg ccgaggaaaa acgtgcgcag atcgagatcc tgcgcaagat
     3841 gttgctggcg atggtcgagg acatccgcgt cgtactgctg cgccttgcct cgcgcacgca
     3901 gacgctgcgc cattacgcgc gacacgaaga cgagctgcgc gtgcaggtgg cgcgcgagac
     3961 gctggacatc tattcgccgc ttgccaatcg cctcggcatc tgggagttga agtgggagct
     4021 ggaggacctg gcgttccgat acctgtatcc cgatacctac aagacgattg ccagccagct
     4081 cgacgagaag cgcatcgagc gagagcagtt catcgccacg gcgatcgaga cgctgcaccg
     4141 cgaactggcg gcgatgggga tcaaggcgga aattcagggg cgtccgaagc acatctacag
     4201 catctggaac aagatgcggc gcaaggacgt gcccttcgac gaggtctacg acgttcgggc
     4261 gttgcgcgtg ctggtcgacg aggtcaagga ctgctacagc gtgcttggcc tcgtgcataa
     4321 cctgtgggcg ccgatctcca aggagttcga cgactacatc tcgcgaccca agggcaacaa
     4381 ttaccgttcg ctccacacag ccgtccactg cgacgatggc cgctcgctcg aaatccagat
     4441 ccgtacccac gagatgcatc gccacgccga acttggcgtc gccgcgcatt ggcgctacaa
     4501 ggaaggcggc gcgcgcggtg atggcgaccg ttacgaggac aagatcgcct ggctgcgcca
     4561 gttgttgtcg tggcgcgacg aagttgccgg atcatccgac tgggtccagc aatacaagca
     4621 ggcggcactg cacgagacga tctacgtgtt gacgccgcag gggcgcgtca tcgacctgcc
     4681 gctcgatgcg acgccggtcg acttcgccta ccgcctgcat accgacctcg gccatcgctg
     4741 ccgcggcgcc aaggttgacg gcgtgctggt gccgctcaat acgccgctga agaccgcgca
     4801 gcgcgtcgag atcgtcgccg ccaaacaggg cggcccgtcg cgcgactggc tcaatcccga
     4861 tctcggatat ctgaccagca agtcggcacg gggcaaggtt cgccagtggt ttgccagtct
     4921 ccagcacgat gagaccgttg ccgaagggcg ggccaccgtg ggcaaggaac tgcagcgcgc
     4981 tgggctgaca agcgtcaatc acgagcagct cgccgcgcgg ctgcattttg ccaaggccga
     5041 cgatctttat gcggcgatgg cgcgcggcga tgtttcaggg aggcagttgg cggccgcggc
     5101 gcgcggtgag gaagcgcagg acgcggcgcc cggggcggca cccgtcgggt cgcgacaaac
     5161 gcgggccagt gacgatgcgc agtcggacat cctcatcgaa ggcgtcggca aggtgttgac
     5221 gcagctggca cgctgctgca agccggcgcc gcccgatccg attggcggtt tcatcacgcg
     5281 cggcaagggg gtgtcgattc atcgcgccga ttgcgcgagc ttcgcccatc tggccgccat
     5341 gcatccggag cgcgtcctcg ataccgaatg gggcggcaag acgcgggggg tcttcggcgt
     5401 tgacatcctg atcgacgccg acgaccggca gggcctgctg cgtgatgtgt cggacgtgct
     5461 ggcgcgcgag cgggtcaatg tcactgcggt caagacccag tcgaagcagg cccgcgcgac
     5521 gatgcgcttc acggccgaga tcgagaacct cgatcggctg caggtggtgc tcaagcagct
     5581 gcagggggtg gccggggtgg tcagcgcgag gcgggcgggc ggcagctagg cgttggcgga
     5641 tcgttgctgc gcagcccagg ccgctaactc gttcggagcc atgggcgggc tgaagaagta
     5701 tccctgggct acgctgcagc cctcgcgctt tagaaattcg acctgctgct cgttttcgac
     5761 gccttcggcg accgtggtca gcgacatcgt tgtcgccagc gcgatgactg agcgcacgat
     5821 cgcggcgtcg ctggcattac gcgtcacgtc gtcgatgaag gactggtcga tcttgagctt
     5881 gttgatcggc aggcggcgca ggtaggacaa ggaggaatac ccggtgccga agtcgtcgat
     5941 cgacagcgag acaccgagtt cgcgcagcat tgccagcagg tgcggcgctt tgccggtctg
     6001 catgaggatc gattcggtga tctcgatttc gatgcgatcg gcgctgacgc cggtgtcacg
     6061 gaggatgtcg gcgacgatat cgatgaaacc ttcgcgttcg atctgccgaa ccgagacgtt
     6121 tacggccagg cgcggcaggt ggaatccctc ggcgtcccac cgttgcagct gctcgcaggc
     6181 ttcgcggaag gcccaggcgc cgaggtcgtt gatgagcccg gtttcctcgg tgatcggaat
     6241 gaatttgggc ggcggaacga ggccgcgctc gggatgggtc cagcgcacca gcgcctcggc
     6301 cccgaccagc gcgccggtgg ccagttcgac ctgcggctgg tactgcagcg tcatttcgcc
     6361 gttgtcgagc gcccgccgta gctgcatttc ggtctgcagg cgctccgagg cgttggcggt
     6421 catttccggg gtgtagaagc ggaccgcgtt gcgtccgaca tcctttgccc ggtacatggc
     6481 ggcctcggca tgcttgacca gcgtatgcac gtcgctgccg tcgatcggga acagactgat
     6541 gccgatgcta gccgagatca gcagttcgtg tccttcaact gtaaatggcg cctccagcgt
     6601 gtcgagcatg tactgcgcga cgagcgctgc gtcgttggca tcgtcgatgt tttcgaccaa
     6661 ggtgatgaac tcatcgccgc ccaggcgagc cagcgtgtcg ccgctgcgca cgccgcggcg
     6721 caggcgcgtg gcgatctcgc tgaggaatgc atcgccggtg tcgtgtccca gcgaatcgtt
     6781 gatcgttttg aagtgatcga ggtcgagaaa gaccagcgca aactgcgttg gctgacgtac
     6841 ggccgcgtct atggccataa ccacgtgctg gttgaaatgg aggcggttgg gcagcccggt
     6901 gacggcgtcg tggtgcgaga ggtactcgat gcgctcctcg gaatcgcgga ggctggagat
     6961 gtcggaaaag acgccaacgt aattcgagac gttgccgaag gaatccttga ccacgctgat
     7021 cgacagccac tcggggtaga tctcgccgtt cttgcgccgg ttgaggattt caccctgcca
     7081 gtggccagtc gactcgagcg atttccacat cgacttgtag aaactcggcg attgcaggcc
     7141 tgacttgagc agccgtggcg tctggccgat cgcctcggct tcgctgaacc cggtgaccac
     7201 ggtgaaggcg cgattgaccg cgaggatggt ctgctcgggg tcggtgatgg tgatgccctc
     7261 gaccgagttc tcgaagacct gcgcagcgag cctgagcttg ccttcgacgg ctaccctatc
     7321 ggcaacctcg cgctgcagtg tctgattggc ggccagcagt tcggcggtgc gatcggtcac
     7381 gcgctggtcg agttcacggt tgttcttcgc gatggtgtcg atcgttgcgt cgagttcccc
     7441 cgattgctgg tcgagtatgt gctgtgcccg cgtgatcagg aggtgcagga ggacgtagag
     7501 gattcccagc agggcgagta ccgcaccgat caccagagag cgcacctcgc gggtggcggc
     7561 gacgaagtcg ctgacgtccg tatagatctc gaagacgccg cggatcgttc cggtcgaatc
     7621 gatcgccgga cggtaggtcg ccaatacatc gaggttctgc agcgtgcctt cgaagccgtc
     7681 gaaggtgttg cggtgcgtga ggtcgctgcg cacgctaccg gccagcgccg acttgacgcc
     7741 cgggttgccg gccgccagcg cgccgacctg cgtcagatcg gtcgaaaaca cggtgaggcc
     7801 gtcaaggttg tagagcttga ccttgatcac cttcgatccc gacatcaggg tgatgacgtc
     7861 ctcgcgcagg cggcgttcgg cgatctcgcg gaggatcgtc tcgctgtcga gcctcggtga
     7921 gcgggcaacc agcgggtcga agcgatgcca gagggcgttg cccagtgttt gtccgagttc
     7981 ggcgttgtga atttccgcct gccgcgacaa ttcgcgcatc gccagatgct ggtagatcca
     8041 gccgagcgcg gcagcaatca tcaggatgca gacgagactg gtcaccgcga aatagcgggt
     8101 caggctgaag cggtgcccgc cggtcagcgg cgctacccgg ctggcgtgcg acagtttcgc
     8161 cggcatcgat ctccccctgt gctgcctttc atcggctgat caacgatcgc cgagcagctt
     8221 cattagatcg tattgtgcac agattggctc gccgcggccg cggcgtggtt cgtaacggcc
     8281 atcggcgagc agctcccacg cctgatgatt atcgcgaaga tagacgcgca gaccctcggc
     8341 gaggacgcgc ttcttcaggc gcggatcgag gatggggaag caaagctcga tgcgacggaa
     8401 gaagttgcgc tccatccagt cggcactcga cagatagaca ttgtcctcgc cttcgttgtg
     8461 gaagcagaag atgcgatggt gttcgagaaa gcgaccgatg atcgagcgga cgcggatgtt
     8521 ctccgacaaa ccggcaacgc caggacgcag cgcgcaggcg ccacgaacca gcaggtcgat
     8581 cttgacgccc gcctgcgatg ccacgtagag cgcatcgatc accttcggct cgagcagcga
     8641 gttcatcttg gccatgatgc gcgccggctt gccggcctgg gcgtttgccg tttcgcgttc
     8701 gatcgccttg atgatgttgg ggtgcaacga aaatggtgcc tgccacagat gatgcagctt
     8761 ctgcgcgcgt ccgagtccgg t
//
LOCUS       DATDAA010000267         3814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_159087,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000267 DATDAA010000000
VERSION     DATDAA010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159087"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="VIS73_08450"
     CDS             <1..144
                     /locus_tag="VIS73_08450"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY5763225.1"
                     /translation="HDFDDVVVIRFADQESLNGWFASPAYQALIPLRLEAADVSLVAY
                     ADD"
     gene            complement(129..1163)
                     /locus_tag="VIS73_08455"
     CDS             complement(129..1163)
                     /locus_tag="VIS73_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY5763226.1"
                     /translation="MRIAAIEDRLAGKGAKLTHRARLLRHWLQDLPDTHGPVRLAHFL
                     PRAVREEWPAVQAELAALAAVSSAHADTAGASRLLVALADGHAVETVLLPRDGVCVST
                     QVGCAVGCGFCMTGRDGLLRQLGSAEIVAQVVLARQRQRVKKVVFMGMGEPAHNLDNV
                     CEAIEALGTHGAIGHKNLVLSTVGDERVFARLPTMSVRPALALSLHTTRAALRATLLP
                     RAPRIDPQELVERCTAWAEETGYPIQYQWTLLAGVNDGDDEIETLAALLRGRYAVMNL
                     IPFNEVEGSGYRRPSPARVEAFALALYRAGVLTKIRDSAGQAIDGGCGQLRSRLAPLR
                     PVRRALQSSA"
     gene            complement(1248..2015)
                     /locus_tag="VIS73_08460"
     CDS             complement(1248..2015)
                     /locus_tag="VIS73_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY5763227.1"
                     /translation="MAIWLRFQVANGEAFGLLEDETVRVHDGDLFGALRDTGVTRALG
                     ELRLLQPCRPGKLIGLWNNFHARAAHEGWDHPPHPLYFVKTDNCYAAHGDEIRRPVSY
                     DGPVVFEGELGIVIGRTCRHIDAAQADDFIFGYTCVNDVTAREILKADPSFPQWVRAK
                     SFDTFGPFGPVIATGIDPDSLTVKTRLNGQLLQDYPVADMFYRPREIVARISQDMTLH
                     PGDVIACGTSLGAGPMESGDRVEVEIAGVGTLSNRFV"
     gene            complement(2029..2760)
                     /locus_tag="VIS73_08465"
     CDS             complement(2029..2760)
                     /locus_tag="VIS73_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY5763228.1"
                     /translation="MDAQYILMFVVVLVAYFVRGLTGFGSGLIAIPILAMFAPLTFVV
                     PVIMTLDFISSFILGGVDRSKTDWTEVGSLLPFGLLGALLGTYVLVNFPTTPVLVALA
                     LFTVFFGARNALGIQPKGAISRLWSIPAGIVGSGAGALFGTSAPPYIMYLTHRLPDKT
                     AARATFSWLFIIDGGFRLILFAIAGLLLTVDALTAIAIGLIPMGIGLFGGNHAHVRIS
                     RDVLLRFVGGFLVFSGGTLLTRVMV"
     gene            complement(2832..>3814)
                     /locus_tag="VIS73_08470"
     CDS             complement(2832..>3814)
                     /locus_tag="VIS73_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5763229.1"
                     /translation="EKMRDALTDFRFAWQTNTYQIGLSVGLVPINDAAQTTIGILSAA
                     DAACYSAKERGRNRVQVYRPNDTELAQRQGEMQWVAKITKAIEEDRLCLYQQAIRSID
                     GSAEGGDHYEILVRMRDESGNLVPPIAFIPAAERYNLMPAIDREIIRKSFAAFDRIYA
                     PGSTRRLTRASINLSGNSLGDEGLTLFIRQQFADHDVRPEQICFEITETAAIANLTHA
                     VSFIRELKLLGCSFSLDDFGSGLSSFAYLKSLPVDTLKIDGGFVKDMPQDPIDTAMVR
                     AINNIGHVMGLKTVAEFVENDVILGMLKEIGVNYAQGYGVAVPAPLDDLS"
BASE COUNT          687 a         1248 c         1248 g          631 t
ORIGIN      
        1 catgactttg acgacgtcgt cgtcatccgc ttcgccgacc aagaatcgct gaacggctgg
       61 ttcgcctcgc cggcgtacca ggcgctgata ccgctgcgcc ttgaggccgc cgacgtctcg
      121 ctcgtcgcct acgccgacga ctgaagcgcc cgccgcaccg ggcgcagcgg cgccagccgt
      181 gaccgcaatt gcccgcagcc gccgtcgatc gcctgcccgg cggaatcccg gatcttggtc
      241 aggacgccgg cacggtagag ggccaaggca aaggcctcaa cgcgcgccgg tgacgggcgg
      301 cggtagccgc tgccctcgac ttcattgaac gggatgaggt tcatcaccgc ataacgaccg
      361 cgcagcagcg ccgccagcgt ctcgatttcg tcgtcgccgt cgttgacgcc ggcgagcagc
      421 gtccactggt actggatcgg atatccggtc tcctcggccc aggccgtgca ccgctcgacc
      481 agttcctgcg gatcgatgcg cggcgcccgc ggcaacagcg tcgcccgcag cgccgccctg
      541 gtcgtgtgca gcgacagggc cagcgccggc cgcaccgaca tcgtcggcaa gcgcgcaaag
      601 acgcgttcat cgccgaccgt cgagagtacg aggttcttgt ggccgatcgc gccgtgcgtg
      661 ccgagcgcct cgatcgcctc gcagacgttg tcgaggttgt gcgccggctc gcccatgccc
      721 atgaacacca ccttcttcac gcgctggcgc tgccgcgcca gcaccacctg cgcaacgatc
      781 tcggcgctgc cgagctggcg tagcaggcca tcgcgcccgg tcatgcagaa gccgcagccg
      841 actgcacagc cgacttgcgt cgagacgcag acgccgtcgc gcggcaggag cacggtctcg
      901 accgcgtggc cgtcggccag cgcgaccagc aggcggctcg cccccgccgt gtcggcgtgc
      961 gcgctgctga cggcagccag cgccgcgagt tctgcctgga cggccggcca ctcctcgcga
     1021 accgcgcgag gcagaaagtg ggccaggcgt accgggccat gcgtatcagg caggtcttgc
     1081 agccagtggc gcaacagccg tgcgcggtgc gttagcttcg cgcccttgcc ggcgaggcgg
     1141 tcttcgattg cggcgatgcg catgcgcggt cggcgtcgtg gtcactcgtg gcgcattgta
     1201 gggcgtcgcc ggggcggcgc ggtgctgggg caccctcggc cggcgagtca tacgaatcgg
     1261 ttgctcagcg tgccgacacc ggcaatctcg acctcgacgc gatcgcctga ttccatcggc
     1321 ccggcaccga gcgaggtgcc acaggcgata acatctcccg gatgcagcgt catgtcctgc
     1381 gaaatccggg cgacgatctc gcgcggccgg tagaacatgt cggccaccgg gtaatcctgc
     1441 aacagctgtc cgttgaggcg cgtcttcacg gtcagcgaat cgggatcgat tcctgtggcg
     1501 atgaccggcc cgaagggccc gaaggtatcg aagctcttcg cacgaaccca ctgcggaaaa
     1561 gagggatcgg ccttgaggat ctcccgcgcg gtgacatcgt tgacgcatgt gtatccgaag
     1621 atgaagtcgt cggcctgcgc ggcatcgatg tggcggcagg tcctgccgat gacgatgcca
     1681 agttcgccct cgaagaccac cggcccgtcg tagctgaccg gccggcggat ctcgtcgcca
     1741 tgcgcagcgt agcagttgtc ggtcttgacg aagtaaagcg ggtgcggcgg atgatcccag
     1801 ccctcgtgcg cggcgcgcgc gtggaaattg ttccacaggc caatcagctt gcccggccga
     1861 cagggttgca gcagtcgcag ctcgccaagc gcccgagtca cgccggtatc gcgcagcgca
     1921 ccgaacagat cgccgtcgtg gacgcgaacg gtttcgtctt cgagaaggcc gaatgcctcg
     1981 ccgtttgcca cctgaaatcg cagccagatc gccatcgctc cgccctcctc agaccatcac
     2041 gcgcgtcagc agcgtcccgc cgctgaagac caggaatccg ccgacaaaac gcagcagcac
     2101 atcgcggctg atgcggacgt gcgcatgatt accgccgaac agcccgatcc ccatcgggat
     2161 cagcccgatc gcgatggccg ttagcgcatc gacggtcaac agcagaccgg caattgcaaa
     2221 gaggataagc cggaagccac cgtcgatgat gaacagccag gagaaggtcg cgcgcgctgc
     2281 cgtcttgtcg ggcagacgat gggtcaggta catgatgtac ggcggtgcgc tggtgccgaa
     2341 cagcgcgccg gcaccactgc cgacgatgcc ggcggggatt gaccacagcc gcgagatcgc
     2401 gcctttcggc tggataccga gtgcgttgcg cgcgccaaag aagaccgtga acagcgccaa
     2461 tgcgacgagc accggggtcg tcgggaaatt caccagcacg taggtaccga gcagcgcgcc
     2521 gagcaggccg aagggcaata gcgagccgac ctcggtccag tcggtcttgc tgcggtcgac
     2581 gccgcccaga atgaaggagg aaatgaagtc gagcgtcatg atcaccggca cgacaaaggt
     2641 cagcggcgca aacatggcaa ggatcggtat cgcgatcagg cccgagccga atccggtcag
     2701 gccgcgaacg aagtacgcga cgaggacgac cacaaacatc aggatgtact gcgcgtccat
     2761 ctatcacctc caccgttgcc tggcattcgg caagctccgg cggggtgccg gctactcatt
     2821 actccggcat atcatgatag atcgtcgagc ggcgccggca ctgcgacgcc atagccctgc
     2881 gcgtaattga cgccgatttc cttgagcatg ccgagaatta cgtcgttctc gacgaactcg
     2941 gcaacggtct tcaggcccat gacgtggcca atgttgttga tggctcgcac catcgccgtg
     3001 tcgatcggat cctgcggcat gtccttgacg aagccgccgt cgatcttcag cgtgtcgacg
     3061 ggcaggctct tgaggtaggc gaacgaggac aggccactgc cgaaatcgtc gagcgagaag
     3121 ctgcagccga gaagcttcag ttcgcggatg aaggaaaccg cgtgcgtcag gttggcgatc
     3181 gcggccgtct cggtgatctc gaagcaaatc tgctcggggc gcacgtcgtg gtcggcgaac
     3241 tgctggcgga tgaacagcgt cagcccttcg tcgcccaagg aattgccgga caggttgatg
     3301 ctggcgcgtg tcaggcgcct agtcgatccc ggggcgtaga tgcgatcgaa ggcagcaaac
     3361 gattttcgga tgatctcgcg atcaatcgcc ggcatcaggt tgtagcgttc ggccgccgga
     3421 ataaaggcga tgggtggcac cagattgccg gactcgtcgc gcatgcggac gagaatttca
     3481 taatggtctc cgccctccgc cgatccgtcg atgctgcgga tcgcctgctg gtagaggcag
     3541 agccggtctt cctcgattgc cttggtgatc ttggccaccc attgcatctc gccctgacgc
     3601 tgcgccagct cggtgtcgtt gggccgatag acctggaccc ggttgcgccc gcgctccttg
     3661 gccgaatagc aggcggcatc tgctgccgac aggatcccga tcgtcgtctg cgccgcgtcg
     3721 ttgatcggca ccaggccgac cgagaggccg atctggtagg tattcgtctg ccaggcgaag
     3781 cggaagtcgg tcagcgcatc gcgcatcttc tcgg
//
LOCUS       DATDAA010000268         2724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_194253,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000268 DATDAA010000000
VERSION     DATDAA010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194253"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VIS73_08475"
     CDS             <1..578
                     /locus_tag="VIS73_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763230.1"
                     /translation="SREYDLLLAEHYDDLVVGLGGTVSPGGAVLRGDLVVTDTDTDTY
                     VQLVTNWNYSWTWRGRNMSGALEYHFNGFGQHDGRYDPASLAGNPDLLARIARGETFT
                     LGRHYVAGSVLVEMTPLWTVTPTLLANVTDPSALLQVVTSFSLSDNMTFLGSLNMPIG
                     PEGSEFGGIETDQGLYLSSGPGIFAQLAWYF"
     gene            complement(589..1146)
                     /locus_tag="VIS73_08480"
     CDS             complement(589..1146)
                     /locus_tag="VIS73_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY5763231.1"
                     /translation="MRIRLARILDELREDHRNLTRLLDLLERECRDVPEEGEPDMELL
                     HDIMHYMTVYPDAIHHPREDLVYTAMREHCVDLAEGLESVPDDHREIAELGATLRKDI
                     EAIISGAAVTRQHLLEDLVNYVGHLRSHMEWEESDLFPRADLLARDNETATLDVSQHM
                     GDDPVFGATTARSFSNLLANLQRAQ"
     gene            1446..>2724
                     /locus_tag="VIS73_08485"
     CDS             1446..>2724
                     /locus_tag="VIS73_08485"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY5763232.1"
                     /translation="MVVSEAQELAIGAQNYAPMQQAEGGIYDIDPELTEYVSEVGNKL
                     AAVSDRPLPYEFVVLNNSVPNAWALPGGKIAINRGLLTELNSEAELAAVLGHEIVHAA
                     AKHSAQQMSRGVLLQSAVLATAMVTGDNSFGHFAVGSAAVGAQLINSTYGRSAESESD
                     KYGMTYMSRAGYDPTGAVTLQETFVRLSEGQDSDWLSGLFASHPPSRARVDANRKTAA
                     SLPAGGTMGVERYEAALAKTMAVKPAYDAYDEGREALAEKKTGEALAMADKAIELFPD
                     EGHFHALRGDARLVDGKYDMAKKNYDSAIRRRDDYFYYYLRRGLAETEIGDLDTAKSD
                     LERSIEMLPTGPAHLALGDIETTRGNDRKAMEHYAIVAKSGGEIGEAATRKLQRLQLP
                     YSPGEVIPRRCDADSAGNLVVSVKNTTPWAIEDV"
BASE COUNT          505 a          828 c          916 g          475 t
ORIGIN      
        1 gcagccggga gtatgacctg ctgctggccg agcactacga cgacctggtc gtggggctcg
       61 gtggcaccgt cagccccggc ggggcggtgc tgcggggcga cctggtggta accgataccg
      121 acacggacac ttacgtgcag ctggttacta actggaacta ctcatggacc tggcgcggca
      181 ggaacatgag cggtgcgctc gaataccact tcaacggctt cggccagcac gatggccgct
      241 acgaccccgc gagcctcgcc ggcaacccgg acctgctggc acgcatcgcg cgcggcgaga
      301 ccttcacgct ggggcggcac tacgtcgcgg gcagtgtcct cgtggaaatg acgcccctgt
      361 ggacggtgac gccgacgctg cttgcgaacg tcaccgaccc cagcgcattg ctgcaagtcg
      421 tgacgtcgtt cagcctgtcc gacaacatga cctttctcgg tagcctgaac atgccgatag
      481 gaccggaggg cagcgagttt ggtggcatcg agaccgacca gggcctgtac ctgtccagcg
      541 ggccgggcat cttcgcgcag ctcgcctggt acttctgacc ggcgcgggct actgggcgcg
      601 ttgcaggttt gcgagaaggt tcgagaaaga cctggccgtc gtggcgccga acacggggtc
      661 gtcgcccata tgctggctca cgtcgagagt cgccgtctcg ttatcccttg cgagcaaatc
      721 ggcgcgcgga aacaggtcgc tttcctccca ctccatgtgg ctgcgcaggt ggccgacata
      781 gttgacgagg tcctccagca ggtgctgccg cgtaacagct gcgccggaga tgatggcctc
      841 gatgtccttg cgcagcgtgg ccccgagttc tgcgatttcg cggtgatcgt ccggtacgct
      901 ctcgagtcct tccgcgaggt cgacacaatg ctcgcgcatc gccgtgtaga ccaggtcctc
      961 gcgcggatgg tggatggcat ccggatagac cgtcatgtag tgcatgatgt cgtggaggag
     1021 ctccatgtcg ggctctccct cttccggaac gtccctgcac tcgcgttcca gcaggtccag
     1081 cagtcgggtg aggtttcgat gatcttcgcg taattcgtcg agaatccggg cgagtctgat
     1141 tcgcattggc aattcacctc tgtttaccgc gcctatcatg cgtgccgtgc gtccgggggg
     1201 taatcgggga atccacctac gcaggggtgg aaagcgtcca caacgaacgg cttcgagggc
     1261 caaccccccc gttgggcgat tcccgcgccg tgtcccctgg ccgtttcaga tagggcgggg
     1321 ccttgatacc gcggcgatcc ggtagcaatg tatagcccat gagaaggaga ctactggcag
     1381 tcgtcgtcct cgccgcctcg atgaccggct gtgccgtcaa cccggttacc gggaagccgg
     1441 atttcatggt tgtcagcgag gcgcaggaac tcgcaatcgg tgcgcagaac tatgccccga
     1501 tgcagcaggc cgagggcggc atctacgaca tcgacccgga actcaccgaa tacgtcagcg
     1561 aagtcggcaa caagctggcg gccgtgagcg accggccgct tccgtatgag ttcgtggtac
     1621 tcaacaactc ggtaccgaac gcctgggcac tgcccggggg caagatcgcc atcaatcgcg
     1681 ggctgctcac cgagctgaat tcggaggcgg agctcgcggc ggtgctgggg catgaaatcg
     1741 tgcacgcggc agcgaagcac tccgcacagc agatgtcccg cggcgtgttg ctgcagagcg
     1801 cggtgctggc gacggcgatg gttaccggcg acaacagttt cggtcatttc gcggtaggca
     1861 gcgcggccgt gggcgcccag ctcatcaatt ccacctacgg gcgcagcgcg gagtcggagt
     1921 ccgacaaata cggcatgacc tacatgtcgc gtgccggcta tgaccctacg ggcgccgtga
     1981 cgctgcagga gaccttcgtt cgcctcagcg aaggccagga cagtgactgg ctcagtggcc
     2041 tgtttgccag ccacccgccc tcgagagcgc gcgtggacgc aaacaggaag acagccgcga
     2101 gtttacccgc gggcggtacg atgggcgtcg agcgctacga ggcagccctg gcgaagacga
     2161 tggcggtcaa gcctgcctac gatgcctacg acgaagggcg cgaagcgctt gcggagaaga
     2221 agaccgggga agcgttggcg atggcggaca aggccatcga gctgtttccg gacgagggtc
     2281 acttccatgc gctgcgcggc gatgcccggc tggtcgacgg caagtacgat atggcgaaga
     2341 agaactacga tagcgcgatc cgtcggcgcg acgactattt ctactattac ctgcgtcgcg
     2401 gtctggccga gacggaaatc ggggatctcg ataccgcgaa gtcggacctg gaacgcagta
     2461 tcgagatgct gccgacggga cctgcgcatc tggctctcgg ggatatcgaa acgacgcgcg
     2521 gcaacgaccg gaaggcgatg gagcactacg cgatcgtcgc gaagagtggc ggggagatcg
     2581 gtgaggcggc gacgcgaaag ctgcagcgcc tgcagctccc gtacagtccg ggcgaagtca
     2641 taccgcggcg ctgtgacgcg gatagcgccg gtaacctcgt ggtatcggtg aagaacacga
     2701 cgccctgggc catcgaggat gtgc
//
LOCUS       DATDAA010000269         2327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_194389,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000269 DATDAA010000000
VERSION     DATDAA010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194389"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..1224
                     /gene="glyQ"
                     /locus_tag="VIS73_08490"
     CDS             289..1224
                     /gene="glyQ"
                     /locus_tag="VIS73_08490"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY5763233.1"
                     /translation="MSSAKDAQSFLSFQDLILRLQQYWAERGCVVMQPYDKEMGAGTF
                     HPATFLRAVGPEPWSAAYVQPSRRPTDGRYGDNPFRLQHYYQYQVVIKPSPDDFQDLY
                     LESLEAIGIDTRVHDIRFVEDNGESPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLEC
                     RPVTGEITYGLERLAMYLQNVESIFDIVWTDGPLGRITYGDVYHQNEVEQSRYNFDEA
                     DVDTLFHDFDHAEHDCERLIEKNLALPAYEKMLAASHLFNLLDARQAISVSERQRYIL
                     RVRTMARAVAEAYYKSREDLGFPMVDSVRARSESA"
     gene            1221..>2327
                     /gene="glyS"
                     /locus_tag="VIS73_08495"
     CDS             1221..>2327
                     /gene="glyS"
                     /locus_tag="VIS73_08495"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY5763234.1"
                     /translation="MTAADLLVEIGTEELPPKALRGLMDAFATNLGNEIDEARLAHGA
                     LHAYASPRRLAVIVDALATGQEDRRVSQKGPPVSVAYGEDGKPLPPATAFARKCGIDV
                     AELGRMETDKGAWLIAETVEQGRPTAELLPALIEKALADLPIPRRMRWGDGDAEFVRP
                     VHWVVLLHGAGVVAGSVMGVPCGDTTMGHRFHSDGPIKIAEPAAYLETLEEQGHVIAD
                     FEVRRGLVCDGVEAMAERAKGHVVEGEALFDEVAALVEWPVPVLGRFDETYLELPREV
                     VVSTLTGHQRYFPIADDSGSLLPSFVTVANLESKEPDKVRDGNERVIRPRLADAAFFW
                     DSDRRKPLSIRREALRDVVYQRGLGSLYDKSSRTA"
BASE COUNT          426 a          745 c          754 g          402 t
ORIGIN      
        1 tgcatttagc gtgccaaaat ggtgcttcgc ttaactattt gtttttgtta ggctgatgac
       61 ccaataacgt ggagagctcg caccagtata gtgcaaaaac aacccggctc gcactaaatt
      121 agtgcggggc ggggtggctg caccttctgg aaggcggcta gtggcacgct ttcggggccc
      181 ggaccgcagc cggggctcgc gggcggccgg ggtgccgggc ccggtggtat ccgagtcggc
      241 tcaactaccc tatactgcgc gggtttgccg caagtgaccc ccgtttcaat gagtagtgcc
      301 aaggacgcac agtcgtttct ctccttccag gacctgattc tccgcctgca gcaatactgg
      361 gcggagcgtg gctgtgtcgt gatgcagccg tacgacaagg agatgggcgc cggtacgttt
      421 catccggcga ccttcctccg cgcggtcggc ccggagccct ggagtgccgc gtacgtgcag
      481 cccagccggc ggcccacgga cggccgttac ggcgacaacc cgttccgcct gcagcactat
      541 taccagtacc aggttgtcat caagccgtcc ccggacgact tccaggacct gtacctcgag
      601 tccctggaag cgatcggcat cgacacgcgc gtgcacgaca tccgtttcgt cgaggacaac
      661 ggggagtcgc cgacgctcgg cgcctggggg ctcggctggg aggtctggct gaacggtatg
      721 gaggtcaccc agttcactta cttccagcag gtgggcggcc tcgagtgccg cccggtcacg
      781 ggcgagataa cctacggtct cgagcgcctc gcgatgtatc tgcagaacgt cgagagcatc
      841 ttcgacatcg tctggaccga cggtcctctg ggccgcatca cctacggtga cgtgtatcac
      901 cagaacgagg tcgaacagtc ccgctacaac ttcgacgaag ccgacgtcga cacgctgttc
      961 cacgacttcg atcacgccga gcacgattgc gagcgcctga tcgaaaagaa cctggcactg
     1021 cccgcttacg agaagatgct cgcggcatcg catctgttca atctcctcga cgcgcgccag
     1081 gcaatctccg taagcgaacg ccagcgctac atcctgcgcg tccggaccat ggcgcgcgcc
     1141 gtcgcggagg cgtattacaa gagccgcgag gacctcggct tcccgatggt cgattcggta
     1201 cgcgcccgga gcgaaagcgc atgacggcgg ccgacctgct cgtcgaaatc ggtaccgagg
     1261 agctgccgcc taaagcactt cgcgggctca tggacgcgtt cgcgacgaac ctcggcaacg
     1321 agatcgacga agcgcgactc gcgcacggtg cgctgcatgc gtatgcgagc ccgcgacgac
     1381 tggccgtaat cgttgacgcg ctggccaccg gccaggaaga ccggcgggta tcgcagaaag
     1441 gcccgcccgt ttcggtcgcc tacggagaag acggcaagcc gctgccgccc gcgaccgcgt
     1501 tcgcaagaaa gtgcggcatc gacgttgccg aacttggccg catggagacg gacaagggtg
     1561 cgtggctcat tgccgaaacc gtcgaacagg gcaggccgac cgccgaattg ctgccggcgt
     1621 tgatcgagaa ggcactcgcc gacctgccga ttccgcgccg catgcgctgg ggggacggcg
     1681 atgcggagtt cgtgcgcccg gtgcactggg tcgttttgct gcacggcgcc ggtgtcgtcg
     1741 ccggctccgt catgggcgtg ccctgcggcg acaccacgat ggggcaccgg tttcattccg
     1801 atggccccat aaagatcgcg gaacccgccg cctacctgga gactctcgaa gagcaggggc
     1861 atgtcattgc cgatttcgag gtccgccggg ggcttgtctg cgatggcgtc gaggcaatgg
     1921 ctgaacgggc gaagggccac gtggtggaag gggaggcgct tttcgacgaa gtggcagcac
     1981 tcgtggaatg gccggtgcct gtcctcggcc gttttgacga gacgtatctc gaactgccac
     2041 gcgaggtggt cgtttcaacg ctcaccggcc accagcgcta cttcccgatc gccgacgaca
     2101 gcggctcgct cctgcccagt ttcgttacgg tagcaaacct cgaaagcaag gaacccgaca
     2161 aggtccgcga cggcaacgag cgcgtgatcc gcccgcgcct cgcggacgcc gcctttttct
     2221 gggacagcga ccggcgcaag ccgctgtcga tccgccgtga ggcactgcga gacgtggtct
     2281 accagcgcgg cctgggaagc ctctacgaca agagcagccg aaccgcc
//
LOCUS       DATDAA010000270         6226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_89454,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000270 DATDAA010000000
VERSION     DATDAA010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89454"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VIS73_08500"
     CDS             complement(<1..760)
                     /locus_tag="VIS73_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763235.1"
                     /translation="MNSETPDSSQALRGTMPRVRAARQPFHGGFRLLRWYSLSSLMAM
                     TVVAIVFAIIGGRFMATETLQRDAVLTAQFIHSMIETESQHWGFAPNASLAEFLDPKV
                     DPSARGFTVAQVKAARAEFYDHIRLLPDELLANVFSPDMVIVWSTNPSLVGREDKENR
                     NLREAFGSKEMVARGHLERDHSKKEQDFVIEPRELFIENYIPLRAVSGEVVAVVEVYK
                     EPKDLLAIIARGERLVWGASALAAALVYMVTFGIV"
     gene            1570..1854
                     /locus_tag="VIS73_08505"
     CDS             1570..1854
                     /locus_tag="VIS73_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY5763236.1"
                     /translation="MNKSELIEAVAKATGESKAAAGRAVDAVIDSVVGAVAKGDNVTL
                     VGFGTFKSTKRAAREGKNPKTGEKIKIPAATVPRFSAGAAFKAAVAKKKK"
     gene            complement(1970..2788)
                     /locus_tag="VIS73_08510"
     CDS             complement(1970..2788)
                     /locus_tag="VIS73_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEY5763237.1"
                     /translation="MCRLYTFRSTHPRKVECELIRAQNSLLAQSRCDGVGEIHPDGWG
                     LGRYDADGRLAVARQPESAFAASGFRAAAAEAFSTNVIAHVRKATVGQIAPENTHPFH
                     HHHWLFAHNGTLAGFPAFRAVLLEEMPVAQRAAIRGTTDSEHLFHYLLGLREREPNRS
                     LIDLLRHGIRRIAMLAEERAPGAHVAFNCFLSDGSETVGVRWGKPLWVVERPRVHPCE
                     VCGGVLHVGEIGSDDYRAVVVASERITLGEDWREIPNQSLFRIGPSVDWRCEPL"
     gene            complement(2829..3182)
                     /locus_tag="VIS73_08515"
     CDS             complement(2829..3182)
                     /locus_tag="VIS73_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763238.1"
                     /translation="MAQDDSQFRGGPQIRVVSIPGGWFGKLLALLLAAAIGVLALTFS
                     LAILAVVFVIGLVIWAYFLIRRALLGSPSASRSQQASRTRRQAPDGEPGGRVIEGEVL
                     GRDDADRQTGPGKRD"
     gene            3300..3770
                     /locus_tag="VIS73_08520"
     CDS             3300..3770
                     /locus_tag="VIS73_08520"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEY5763239.1"
                     /translation="MLGSIQDFFRRQIEPKLADPGAADDSGLKLATAALLVEMMRMDR
                     SVLDAERASVVATLTREFGVAASAIDELLALAEAEAQEATDYFQFTSLINRSASAAQK
                     VRIIESMWRVAFADGHLDDHEAHLMKKVAGLLHIPHADYINAKTRAREGVARKG"
     gene            3848..4615
                     /locus_tag="VIS73_08525"
     CDS             3848..4615
                     /locus_tag="VIS73_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY5763240.1"
                     /translation="MLELLSDPQTWMAFATLTALELVLGIDNVIFISILVDRLPPEQR
                     ERARRIGLFLAMFMRIALLLLLAWIVGLTKPLFHLFSLGFSGRDLILIAGGFFLVWKS
                     THEVHQLLEGIEGEASSAVKATFTAIITQIIIIDLVFSLDSIITAVGMVREVEIMVAA
                     VVVSVALMMMYARIIGEFVSAHPTIKMLALSFLMVVGVTLIVEGFGYHVPKGYIYFAM
                     AFSVGVEVLNIRLRKKRTATPVKLHEAYTAATDEAGK"
     gene            4622..>6226
                     /locus_tag="VIS73_08530"
     CDS             4622..>6226
                     /locus_tag="VIS73_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5763241.1"
                     /translation="MLRLSELTLPLDHPPEALHTAAASRLRIAPDAIVALHVARRGVD
                     ARKRSAIRFVYSLDLELDDEPAVLAAHAGDPQLRPTPDTRYRFVATADRAPARRPLIV
                     GFGPAGLFAALILAQSGFRPIVLERGKAVRERTKDTWDLWRRHQLHAESNVQFGEGGA
                     GTFSDGKLYSQIRDPRHLARKVLEEFVKAGAPEEILFVSKPHIGTFRLVSMVEKIRAE
                     IIALGGEIRFSSRVDDLLIDNGQVRGAVLAGGETLAADHVVLALGHSARDSFEMLAAR
                     GVFVEAKAFAVGFRIEHPQSLIDAARFGSAAGNPLLGAADYKLVHHAQQGSGRGRAVY
                     SFCMCPGGQVVAATSEPGRVVTNGMSQYSRAERNANAALVVNVEPTDYATWGKGPGDP
                     LAGIAFQRDLESRAFALGGENYDAPAQRVADFLAGRASTALGSVIPSYQPGVKPTDLS
                     AALPDFAIAAIREAIPAFAKKIRGYDLPDAVLTGVETRTSSPLRIRRGGDFQSINTRG
                     LYPAGEGAGYAGGILSAAIDGIEVAEA"
BASE COUNT         1130 a         2126 c         1936 g         1034 t
ORIGIN      
        1 ggacgatgcc gaaggtcacc atataaacca gggccgcggc cagagcactt gccccccaga
       61 ccaagcgctc gcctcgtgcg atgattgcca acaggtcctt gggctccttg tatacctcga
      121 cgaccgcgac gacttcgccg gaaaccgcgc gcagcggaat gtagttctcg atgaagagtt
      181 ccctgggttc gatgacgaag tcctgctcct tcttactgtg gtcgcgctcg aggtgtccgc
      241 gggcgaccat ttccttcgag ccgaacgcct ctcgcagatt gcggttttcc ttgtcctcgc
      301 gtccgaccag cgagggattg gtcgaccaga cgatgaccat gtcgggcgaa aagacattgg
      361 cgagcaactc gtcgggcaga agccggatgt gatcgtagaa ctcggcgcga gctgctttca
      421 cctgcgcgac ggtaaaaccc cgcgcgctgg gatcgacctt cggatcaaga aactcggcaa
      481 gcgatgcatt gggtgcgaaa ccccagtgct ggctttcggt ctcgatcatg ctgtggatga
      541 attgcgcggt gaggacggcg tcgcgctgca gcgtctcggt cgccatgaaa cgcccgccga
      601 tgatggcgaa gacaatcgcc accacggtca tcgccatcag gctagacagc gaataccagc
      661 gcagcagccg gaagccgccg tgaaagggct gccgagcggc acgcacgcgc ggcatcgtgc
      721 cgcgcagcgc ctgcgaagag tccggtgttt cgctgttcat gaagcgaatg taggtgaaac
      781 gcagctaaca ccggggaatt atccccaata cttgtccaag tttgtcacgg tttccgcatt
      841 attgggggaa tgcacccagc gctccgtcga tcccccaccc gcgctttccc ggcaagccga
      901 cgcgagtcct tggcctgacg caccgtggca ctacgttgcc ggcggcgaag gacgcctccg
      961 aaatcgacgc gaccaagagc tcgcgtcgag cacgaacagc atccggcaag tgaccggaac
     1021 ccagcctcgc tggcgatgga cgcaccggcc gcgcatcgcc ggtcgcgaca gaaccgacta
     1081 gcgccaaacg gaatcgcggt cgatgccggc aacactcgtg cgcctgccgg gcgagcggtg
     1141 tttgcgccag cacatcacca cccgtggcca gtgcccgacc caggtccgat tacggcgagc
     1201 accgcatcga ccagtcaccg cagcgaaatc cgcgccagcg cccttaccgt cgccgtggcc
     1261 gcgcccttcg ccgaattcat cgaatatgct gcacaacgtg cggccgaatc gcttgccaga
     1321 gaatgcgatt cgacggatga aagcaacgct gcaacagacc tttagcgccg caagttcaag
     1381 cgacgacgcc ggttcgctcg tgcgaaacgc ccggccggca acggcttccg gcggttgcat
     1441 ggattctgca ttgacgcaac agtcaagaaa aaaattttgc cgaattggcc gaaagacaga
     1501 ttggcaggcg cctccagctc tgttttaatg gcgctgcctt tttcactcac acccaaaaaa
     1561 ggagcacaca tgaacaaatc ggaactgatc gaagccgtcg ccaaggctac cggcgagagc
     1621 aaggccgcag ctggccgcgc cgttgatgcg gtgattgatt cggtcgttgg cgcggttgcc
     1681 aagggcgaca acgtcacgct ggttggcttc ggcaccttca agtcgaccaa gcgcgcagca
     1741 cgcgaaggca agaacccgaa gactggcgag aagatcaaga ttccggcggc aaccgtgccg
     1801 cgtttctcgg ctggcgccgc cttcaaggcc gccgtcgcca agaagaagaa gtaatcgcag
     1861 gcagccaagg ctgcacgcat gaactggccg gggcgacccg gccttttctt ttgcacaacc
     1921 gccggcacgc agggtagccg catttcgcca tcccgaccgg catcgcgact cagagtggct
     1981 cgcaacgcca gtcgacgctc gggccgatgc gaaacagcga ctggttggga atctcgcgcc
     2041 agtcctcgcc caacgtgatc cgctcggaag cgacaaccac ggcccggtag tcatcgctgc
     2101 caatctctcc gacatggagc acacctccgc agacctcgca ggggtgcacg cgtggccgct
     2161 cgaccaccca aagcggcttg ccccagcgca cgccaacggt ttcgctgcca tcactcagaa
     2221 agcagttgaa ggcgacatgc gcgccgggcg cccgctcctc ggcgagcatc gcaattcgcc
     2281 ggatgccatg ccgcaggaga tcgatgaggc tgcggttcgg ctcacgttcg cgcagcccca
     2341 gcaggtagtg gaacaagtgc tcgctgtcgg tcgtgccgcg gattgcggcg cgctgtgcca
     2401 ccggcatttc ctccagcagc acggctcgga aggcggggaa gccggccaac gttccgttgt
     2461 gtgcgaacag ccagtggtgg tgatggaagg ggtgggtgtt ctccggcgcg atctggccga
     2521 ccgttgcctt gcgcacatgg gcgatgacgt tggtcgagaa ggcctcggcg gcagcggcac
     2581 gaaagccgct ggcggcgaag gccgattcgg gctgccgggc aacggcgagc ctgccgtcgg
     2641 catcgtagcg gcccaacccc cagccgtcgg gatggatttc cccgacgccg tcacagcggc
     2701 tttgcgcgag cagcgagttc tgcgcgcgga tcagttcaca ctcgaccttg cgcgggtgtg
     2761 tcgagcgaaa cgtatagagg cggcacatgg ctggtcgggt gtcgtgcctc ttctggcggt
     2821 cgcctgaatc agtcgcgctt gccgggaccc gtttgccgat cggcatcatc gcggccgagc
     2881 acttcgccct cgatgacgcg cccgcccggc tcgccgtcgg gcgcctgccg gcgcgttcgc
     2941 gaagcctgct gcgagcggga tgccgatggc gacccgagca gtgcgcggcg gatcaggaag
     3001 taggcccaga tcaccaggcc aatgacgaac accaccgcga gtatcgccag cgagaaggtc
     3061 agcgccagga cgccaatcgc cgccgcgagc agcagcgcca gcagcttacc gaaccagccg
     3121 ccggggatgc tgaccacgcg aatctgcggg ccgccacgga attgcgaatc gtcttgcgcc
     3181 atgttccaga agtgtatttc gttcggcgcg catggtaaca gttcggcctg cccatcgcgg
     3241 ccacgaggcg gcggccgcac tatactttgg cctcaccatc aagcgattgt cgaaccatca
     3301 tgctgggaag cattcaggac ttcttccgcc gccagatcga accgaagctc gccgatcccg
     3361 gcgccgctga cgactccggc ctgaagctgg cgactgcggc gctgctggtc gagatgatgc
     3421 gaatggatcg cagcgtgctc gatgccgaac gcgccagcgt ggtcgcgacg ctgacccgcg
     3481 agttcggcgt cgcagcgtcg gcgatcgatg agttgctggc gctggcagag gccgaagccc
     3541 aggaagcgac cgattacttc cagttcacct cgctgatcaa tcgcagcgcc tcggcggcgc
     3601 agaaggtcag gatcatcgaa tcgatgtggc gcgtcgcctt cgccgacggg cacctcgacg
     3661 accacgaggc gcacctgatg aaaaaggtcg ccggcctgct gcacatcccg cacgccgatt
     3721 acatcaacgc caagacccgc gcccgcgaag gcgttgcccg caagggctaa gcgacgcact
     3781 gtggcagaat cgcgcgtctg gcacgacggc gctgccggcg cgacgaaatg cctgaacgga
     3841 gactgacatg cttgagctgt tgagcgaccc gcaaacctgg atggcgttcg cgacgctcac
     3901 ggcgctcgag ctggtgcttg gcatcgataa cgtcatcttc atctccatcc tcgtcgaccg
     3961 cctgcccccc gagcagcgcg aacgggcgcg ccgtatcggc ctcttcctcg cgatgttcat
     4021 gcgcattgcg ctgctgctgc tgctcgcctg gatcgtcggc ctgaccaagc cgctcttcca
     4081 cctcttcagc ctcggcttct cggggcgcga ccttatcctc atcgccggcg gcttcttcct
     4141 cgtctggaag agcacgcacg aggtccatca actgctcgaa ggcatcgagg gagaagcctc
     4201 aagcgcggtc aaggcgacct tcaccgcgat catcacgcag atcatcatca tcgacctcgt
     4261 gttctcgctc gactccatca tcaccgcggt cggcatggtc cgcgaggtcg agattatggt
     4321 cgccgccgtc gtcgtttcgg tggcgctgat gatgatgtac gcgcgcatca tcggcgagtt
     4381 cgtctcggca cacccgacga tcaagatgct cgcactgtcc ttcctcatgg tggtcggcgt
     4441 cacgctgatc gtcgagggct tcggctacca cgtgcccaag ggctacatct atttcgcgat
     4501 ggccttctcg gtcggcgtcg aggtgctcaa catccgcctg cgcaagaagc gcacggcgac
     4561 gccggtcaaa ctgcacgagg cctataccgc cgcgaccgac gaagcgggca agtaacacac
     4621 cgtgctgcgc ctctccgagc tgacgctacc gctcgaccac ccgcctgagg cgctgcacac
     4681 ggccgccgcc agccggctgc ggatcgctcc cgacgcaatc gtggcgctgc acgtcgcccg
     4741 ccgcggcgtc gatgcgcgca agcgctcggc gatccgcttc gtctactcgc tcgatctcga
     4801 actcgacgac gaaccggccg tccttgccgc ccacgccggc gacccgcagc tgcggccgac
     4861 gcccgacacc cgctatcgct ttgtcgccac ggccgacagg gcaccggctc gacgcccgct
     4921 gatcgtcggc ttcggcccgg ccggcctctt tgccgcgctg atcctcgccc agtcgggatt
     4981 tcgcccgatc gtgctcgaac gcggcaaggc ggtgcgcgaa cgcacgaaag acacctggga
     5041 cctgtggcgc cgccaccagc tgcacgccga gtcgaatgtg cagttcggcg agggtggcgc
     5101 cggcaccttt tccgatggca agctctacag ccagatccgc gacccgcgcc acctggcgcg
     5161 caaggtactt gaggaattcg tcaaggcagg cgctcccgag gaaatcctct tcgtctcgaa
     5221 gccgcacatc ggcaccttcc gactggtatc gatggtcgag aagatacgcg ccgaaatcat
     5281 cgcgctcggc ggcgagatcc gcttctcgtc gcgcgtcgac gacctgctga tcgataacgg
     5341 tcaggttcgc ggcgccgtgc tcgccggcgg agagacactc gccgccgatc acgtggtgct
     5401 cgcacttggc cacagcgccc gtgacagctt cgaaatgctc gccgcgcgag gtgtcttcgt
     5461 cgaagcgaag gccttcgccg tcggcttccg catcgagcat ccgcaatcgc tgatcgacgc
     5521 cgcgcgtttc ggcagcgccg ctggcaaccc gctgctcggc gccgccgact acaagctggt
     5581 ccatcacgcg cagcagggca gcggccgcgg tcgcgccgtc tacagtttct gcatgtgccc
     5641 gggcggccag gtcgtcgccg cgacctcgga accggggcgc gtcgtcacca acggcatgag
     5701 ccagtactcg cgcgccgagc gtaacgccaa cgcggcgctg gtggtgaacg tcgagcccac
     5761 cgactacgca acttggggca aggggccggg cgacccgctc gccggcatcg ccttccagcg
     5821 cgatctcgag tcgcgcgcct tcgccctcgg cggcgaaaac tacgacgccc cggcgcagcg
     5881 cgttgccgac ttcctcgccg ggcgcgcatc gacggcgctg ggcagtgtca tcccctccta
     5941 ccagcccggc gtcaagccga ccgatctcag cgctgcattg cccgacttcg caatcgccgc
     6001 gatccgcgaa gcgatccccg ccttcgcaaa gaagattcgc ggctacgacc tgcccgacgc
     6061 cgtgcttacc ggcgtcgaga cgcgcacctc ctcgccgctg cgcatccggc gcggcggcga
     6121 tttccagtcg atcaacacgc gcggcctgta cccagccggc gaaggcgctg gctacgccgg
     6181 cggtatcctg tcggcagcga tcgacggcat tgaggttgcc gaagcc
//
LOCUS       DATDAA010000271         4830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_89584,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000271 DATDAA010000000
VERSION     DATDAA010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89584"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..137
                     /locus_tag="VIS73_08535"
     tRNA            62..137
                     /locus_tag="VIS73_08535"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:95..97,aa:Trp,seq:cca)
     gene            174..524
                     /gene="secE"
                     /locus_tag="VIS73_08540"
     CDS             174..524
                     /gene="secE"
                     /locus_tag="VIS73_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY5763242.1"
                     /translation="MVDNLKLGLSLLLLGSGIAGFYALDDQAMVLRVLAVLGGVAAAV
                     AVAWFTAPGQQFFVFAQEATSESRKVVWPSRKETVQTTAMIFAFAVVMAILLWLADKG
                     LEWVLYDLVLGWRK"
     gene            521..1054
                     /gene="nusG"
                     /locus_tag="VIS73_08545"
     CDS             521..1054
                     /gene="nusG"
                     /locus_tag="VIS73_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY5763243.1"
                     /translation="MMMRWYVVHAYSGFEKSVQRALVDRIARSGMQDKFGQILVPVEE
                     VVEMKGGQKSISERKFFPGYVLVEMEMTDDTWHLIKSTPKVTGFVGGVGNKPTAISEK
                     EVEKIMQQMQEGVEKPRPKVLFEVGEMVRVKDGPFTDFTGSVENVNYEKSKISVSVTI
                     FGRSTPVELEFSQVEKS"
     gene            1150..1581
                     /gene="rplK"
                     /locus_tag="VIS73_08550"
     CDS             1150..1581
                     /gene="rplK"
                     /locus_tag="VIS73_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY5763244.1"
                     /translation="MAKKIVGYIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     QTQGVEPGLPIPVVITAFADKSFTFVMKTPPASILIKKAAGVQKGSPKPHTDKVGKLT
                     RAQCEEIAKAKMPDLTAADMDAAVRTIAGSARSMGITVEGL"
     gene            1583..2278
                     /gene="rplA"
                     /locus_tag="VIS73_08555"
     CDS             1583..2278
                     /gene="rplA"
                     /locus_tag="VIS73_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY5763245.1"
                     /translation="MAKATKRQQALQGKVDRVKVYSLSEALALAKQTATAKFDESIDL
                     AVNLGVDPKKSDQVVRGSVVLPAGTGKTVRVAVFAQGEKAEAAKAAGADIVGFEDLAA
                     EVKAGTINFDVVIATPDAMRVVGQLGQILGPRGLMPNPKVGTVTMDVTTAVKNARAGQ
                     VQYRTDKAGIVHCTIGRASFGVEQLEANAKAMFDALNKAKPAAAKGQYLKKLCVSSTM
                     GPGIRVDQATIAG"
     gene            2563..3066
                     /gene="rplJ"
                     /locus_tag="VIS73_08560"
     CDS             2563..3066
                     /gene="rplJ"
                     /locus_tag="VIS73_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEY5763246.1"
                     /translation="MGLNLEEKKAVVAEVSAQVANAQTIVLAEYRGVEVGDLTRLRAS
                     ARKSGVYLRVLKNTLVRRAVADTSFAPLAEQMTGPLIYGISTDPVAAAKVLNDFAKGN
                     EKFELRAGSYSGKLLDKSAVQALASIPSRDELLAKLLGIMQAPVSGFAGALAALAKQR
                     EEQGAAA"
     gene            3107..3481
                     /gene="rplL"
                     /locus_tag="VIS73_08565"
     CDS             3107..3481
                     /gene="rplL"
                     /locus_tag="VIS73_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEY5763247.1"
                     /translation="MAISKEDILEAVGNMTVMDLNDLVKAFEEKFGVSAAALAVAAPG
                     AGGGAAAAEEQTEFTVVLAATGDKKVEVIKVVRAATGLGLKEAKDLVDGAPKPVKEGI
                     PKADAEALKKQLEDAGAKVEVK"
     gene            3724..>4830
                     /locus_tag="VIS73_08570"
     CDS             3724..>4830
                     /locus_tag="VIS73_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY5763248.1"
                     /translation="MTYSFTEKKRIRKSFAKRANVQDVPFLLATQLESYTSFLQAATA
                     PGRRKTQGLQAAFSSIFPISSHSGNARLEFVSYQLAEPIFDVKECQQRGLTYASALRA
                     KVRLVIMDREAATETIKEVKEQEVYMGEIPLMTTTGSFVVNGTERVIVSQLHRSPGVF
                     FEHDRGKTHSSGKLLFSARVIPYRGSWLDFEFDPKDFLFFRIDRRRKMPVTILLKAIG
                     LTNEQILAEFFEFDTFHITKKGIELELVAERLRGQTLPFDILDKAGKPIVAKEKRITA
                     KHIREMDAAGLKRLPVPDEYMLGRVLGNTIIDTESGEIVVNANDEITETELAKLREAE
                     VAEISTLYTNDLDRGSFISNTLRTDETASKQAARI"
BASE COUNT          958 a         1406 c         1558 g          908 t
ORIGIN      
        1 ggtcggagcg tagttgctcg gagcgccccg gaggccgggg cgctcttggt gtctttgccg
       61 caggggcata gctcaattgg cagagcgtcg gtctccaaaa ccgaaggttg ggggttcgag
      121 accctctgcc cctgccactg gaaggaaaga tgggcgcttg cgcccttgag gtcatggtcg
      181 acaatctgaa gttgggtctc tcgctgttgc tgctcggatc cggtatcgcc ggcttttatg
      241 cgctggacga tcaggcaatg gtcctgcgtg ttctcgccgt gcttggcggt gtcgcggctg
      301 cggtcgcggt tgcctggttc accgcgccgg gccagcagtt cttcgtcttt gcccaggaag
      361 cgacctccga gtcgcgcaag gtcgtctggc cgtcaaggaa agagacggtg cagaccaccg
      421 ccatgatctt cgcctttgcc gtggtgatgg cgatcctcct ctggctggcc gacaagggcc
      481 ttgagtgggt cctctacgac ctcgttcttg gctggaggaa gtgatgatgc gttggtatgt
      541 ggtgcacgcc tactcaggct tcgaaaagag cgtccagcgg gcgttggtcg accgcatcgc
      601 gcgttcgggc atgcaggaca agttcgggca gattctggtt ccggttgagg aagtcgtcga
      661 aatgaagggc ggccagaaga gcatttccga acggaagttc ttcccgggtt acgtgctggt
      721 cgagatggag atgaccgacg acacctggca cttgatcaag agcacgccca aggtcaccgg
      781 cttcgttggc ggcgtcggca ataagccaac cgcgatttcc gaaaaggaag tcgagaagat
      841 catgcagcag atgcaggagg gcgttgagaa gccgcgcccg aaggtgctgt tcgaggtagg
      901 cgagatggtt cgcgtcaagg acggcccgtt caccgatttc accggcagcg tcgagaacgt
      961 caactacgag aagagcaaga tcagcgtctc ggtgacgatt ttcgggcgct cgacgccggt
     1021 cgagctggaa ttcagtcagg tcgagaagag ctgactgcgg tagggccccg gcgacggggc
     1081 ggttccccgc acgcggggtt ttttcgggga gcgcctgacg gcgcgttcgc acccaacagg
     1141 agctgaatca tggcaaagaa aatcgtcggc tacatcaagc tgcaagtgcc ggccggcaag
     1201 gcgaatcctt cgccgccgat cggaccggcg ctgggccagc gcggtctcaa cattatggaa
     1261 ttctgcaagg ccttcaatgc ccagacgcag ggcgttgagc caggcctgcc gattccggtc
     1321 gtcatcaccg cttttgcgga caagtcattc acctttgtga tgaagacgcc gccggcgtcg
     1381 attctgatca agaaggccgc tggcgtgcaa aagggctcgc cgaagccgca caccgacaag
     1441 gttggcaagc tgacgcgtgc gcaatgcgaa gagatcgcca aggcgaaaat gcccgatctc
     1501 acggccgccg acatggacgc tgcggtgcgg acgatcgctg gttctgcccg cagcatgggc
     1561 atcacggtgg agggtctctg aaatggcgaa agcgacaaag cgtcagcagg cgctccaggg
     1621 caaggttgat cgcgtgaagg tctactcctt gagcgaggcg ctggcactgg ccaagcagac
     1681 cgcgacggct aaattcgacg agtcgattga cctcgcggtc aatctcggcg ttgatccgaa
     1741 aaaatccgat caggtcgtcc gcggctcggt tgtgctgcct gcgggcaccg gcaagaccgt
     1801 acgcgtcgcc gtgttcgcgc agggcgagaa ggccgaggcg gccaaggctg ccggtgccga
     1861 tatcgtcggc tttgaggatc tggctgccga ggtcaaggct ggcacgatca atttcgacgt
     1921 ggtcatcgcc acgccggacg cgatgcgcgt ggtcggccaa ctcggtcaga ttctcggccc
     1981 gcgtggcctg atgccgaacc ccaaggtcgg cacggtgacg atggacgtga cgacggcggt
     2041 caagaatgcc cgcgccggtc aggtccagta ccgcaccgac aaggccggca tcgtgcattg
     2101 cacgatcggc cgcgcctcct tcggcgtcga gcagctggaa gccaacgcca aggccatgtt
     2161 cgacgcactg aacaaggcca agcccgctgc agcgaagggg cagtatctga agaagctttg
     2221 cgtgtcgagc acgatgggcc cgggaatccg ggtcgatcag gcaacgatcg ccggctgatc
     2281 atcacgcggc gagcgttcgc cgcgtaccga actggatgcg gcgaatcata tcgtcgcgtc
     2341 aacgaaactt tgggccgggg cggcagccgt atcgtcgttc ccggatcgtc aaagaccgca
     2401 ggtgtctccg gttcgccggg acttaagcgt gaaagcggcc tgcgcagatg gtgtgcccag
     2461 aacaggattt gccggcttgc cggctcgctc ctgatccagg tcgccgtggg tgcggcggcg
     2521 cgcaagcgtt gccgtgtgtg aacttgatag gagaaaagac ccgtgggtct caatctggaa
     2581 gagaaaaaag ccgtcgtggc tgaggtgtcg gcgcaggttg ccaatgccca gacgattgtg
     2641 ctggctgaat accgtggtgt agaggtcggt gacctcacgc gcctgcgtgc ttcggcccgc
     2701 aagtcgggcg tctacctgcg tgtgttgaag aacacgctgg tgcgccgcgc cgtcgccgat
     2761 acgtccttcg ctcccttggc cgaacagatg accggtccgc tgatctatgg catttcgacg
     2821 gatccggttg cggcggcaaa ggtgctgaac gactttgcca aaggcaacga gaaattcgaa
     2881 ctgcgtgccg gttcgtattc gggcaagttg ctggacaagt cggctgtaca ggcgctcgcc
     2941 tcgattccga gtcgcgacga attgctcgcc aaactgctgg gcatcatgca ggcaccggtg
     3001 tccggctttg ccggcgcgct ggcggctctg gccaagcagc gcgaagagca gggcgccgca
     3061 gcctgaacga cgaactttga aaatctgatc ttaggagtta tttgaaatgg caatcagcaa
     3121 ggaagacatt ctggaagccg tcggcaacat gacggtcatg gatctgaacg atctggtcaa
     3181 ggcgttcgaa gagaagttcg gcgtgtccgc cgcggctctg gccgtcgctg cgccgggtgc
     3241 aggcggtggc gctgccgctg cagaagagca gaccgagttt acggtcgttc tcgccgcaac
     3301 cggtgacaag aaggtcgagg tcatcaaggt cgttcgcgcg gctaccggtc ttggcctcaa
     3361 ggaagccaag gacctggtcg atggcgcacc gaagccggtc aaggaaggca ttcccaaggc
     3421 cgacgcggaa gcgctcaaga agcagctcga ggacgcgggc gccaaggtcg aggtcaagta
     3481 attccgatct cgatgatcgg gctgggggcg gttttcgcca ccagcccgtt tgtgctttcc
     3541 ggccgtcgga ggtgtttcgg cgtattgaat gacgaacagt ttgaactgtc gcgtggatac
     3601 acgagagggt ctggcaacag caaacgctgc cagattctgc cgtgtgcaca tgcctcgatc
     3661 gatcctgcga acgggaacgc actgtcccta cgcatcacac tttgactgca tctcggagcg
     3721 aacatgacct actccttcac cgagaagaaa cgtatccgca agagcttcgc caagcgcgcg
     3781 aacgttcagg acgttccctt tctgcttgcg acccagcttg agtcctacac ctccttcctc
     3841 caggccgcca ccgcgccggg ccggcggaag acccagggcc tgcaggcggc tttctcgtcg
     3901 atcttcccga tctcctcaca ctcggggaat gcccgactcg aattcgtcag ctatcagctg
     3961 gccgaaccga tctttgacgt caaggaatgc cagcagcgtg gcctgaccta cgcttcggcg
     4021 ctgcgcgcca aggttcgcct ggtgatcatg gatcgcgaag cggcgaccga gacgatcaag
     4081 gaggttaagg agcaggaagt ctacatgggc gaaatcccgc tgatgacgac cactggttcc
     4141 ttcgtcgtca acggtaccga gcgggtgatc gtcagccagc tgcaccgttc gccgggcgtc
     4201 ttctttgagc acgaccgggg caagacccac agttcgggca agctgctgtt ttcggcgcgc
     4261 gtgattccct accgcggttc ctggctcgac ttcgagttcg acccgaagga tttcctgttc
     4321 ttccggatcg accgtcggcg caagatgccg gtgacgatcc tgctcaaggc aatcggcctg
     4381 accaacgagc agatcctcgc cgagttcttc gagttcgata cattccacat caccaagaag
     4441 ggcatcgagc tcgaactggt cgccgagcga cttcgtggcc agaccctgcc cttcgacatc
     4501 cttgacaagg ccggcaagcc gatcgtcgcc aaggaaaagc ggatcaccgc caagcacatc
     4561 cgcgaaatgg atgcggctgg cctcaagcgt ttgccggtgc ccgacgagta catgctcggt
     4621 cgtgtgctgg gcaacacgat catcgatacc gagtcgggcg agatcgtcgt caatgcgaac
     4681 gatgaaatta ccgagaccga gctcgcaaag ctgcgcgaag ccgaagtcgc cgagatcagc
     4741 acgctctaca ccaatgacct cgatcgtggt tcgttcatat cgaacacgct gcgaaccgac
     4801 gaaaccgcct cgaagcaggc cgcgcgcatc
//
LOCUS       DATDAA010000272         3028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_439371,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000272 DATDAA010000000
VERSION     DATDAA010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439371"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VIS73_08575"
     CDS             complement(<1..678)
                     /locus_tag="VIS73_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5763249.1"
                     /translation="MNYWLLLRGLGRDARHWGVFPRQLRDALGEGEAEVLTMDLPGNG
                     QRNDEPSPASVAAMVDDCRSRLAPKLCGEPLQLLALSLGGMVALDWLCRHPDEIARCV
                     LINTSLGGVNPVYQRLRPRQYWRLLRLLLGAAPESAEALILAMTSNRPDKQLLAEWVT
                     YHRERPVERGNLLRQLQAAARFRAPTVMPPTPVLLLASEHDQLVDVACSRRLSRRYHW
                     PLIVHGSA"
     gene            1076..2437
                     /gene="nifN"
                     /locus_tag="VIS73_08580"
     CDS             1076..2437
                     /gene="nifN"
                     /locus_tag="VIS73_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifN"
                     /protein_id="HEY5763250.1"
                     /translation="MAKVLPGKKACTVNPLKMSAPIGAALAFLGMDRALPLLHGSQGC
                     TAFGLVLFVRHFRETIPLQTTAMSEVTTILGGMDNVEQALLNIKKRADPALIGICSTG
                     ITEAKGDDVAGFLTLIRERHPELAGTEVVYVSTPDFVGGFADGWAKAVSAMVRDLVPA
                     TPVRTPWQVNILAGSHLSVGDIEEIRETVEAFGLTPIILPDLSGSLDGHIPENFLPHT
                     LGGTRLEDVRRMGASAMTIAIGESMRPAADLLWKQAGVPFEVLDRLTGLAACDGFIRM
                     LSDLSHNEVPSRYRRQRSQLQDAMLDGHFYFGGQKVAIGAEPDLLYALSHFFADLGAE
                     VVCAVAPQDAPILERVAAETVQVGDLEDFEQGATGAGLLVTHSHGRQAAARLGIPFYR
                     VGIPTFDRLGAAHRCTVGYRGTRNLIFEVGNLFIERLHAHENAVETADVTGNAHAPSV
                     AGH"
     gene            2460..2879
                     /gene="nifX"
                     /locus_tag="VIS73_08585"
     CDS             2460..2879
                     /gene="nifX"
                     /locus_tag="VIS73_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifX"
                     /protein_id="HEY5763251.1"
                     /translation="MKVAFATEDLKTVDAHFGWARQLAVYEIGPESHALLEVVQFDGD
                     LKEDGSEDKLEPKLNAIRDCAIVYVAAIGGSAAAKVVNLRVHPIKVTQPEAIADIIVK
                     LQGVLRGTPPPWLRKVLLKDKPRSFDFDDEESEAHHG"
BASE COUNT          544 a          960 c          966 g          558 t
ORIGIN      
        1 ggcgctgcca tggacgatga gcggccagtg ataacggcgc gacaggcggc gcgagcaggc
       61 aacatcgacc agttgatcgt gttcgctggc gagcagcaag accggcgtcg gtggcatcac
      121 cgtcggcgcg cgaaagcggg cagccgcctg caactgccgc agtagattgc cgcgctcgac
      181 cgggcgctcg cggtggtatg tcacccactc ggcaagcaac tgcttgtcgg ggcgattgct
      241 ggtcatcgcc aggatcagtg cttcggccga ctcgggcgcg gcgccgagca ggagccgcaa
      301 caatcgccag tactgccgtg gacgcagccg ctggtaaacc ggattgacgc cgccgaggct
      361 ggtgttgatg agcacacaac gcgcaatctc gtccggatgg cggcacagcc agtcgagcgc
      421 aaccatgccc ccgagcgaca gtgcaagcag ttgcagtggt tcgccgcaaa gcttcggcgc
      481 aaggcgagag cggcagtcat cgaccatcgc cgcgaccgat gccgggcttg gttcgtcatt
      541 gcgctgaccg ttgccgggga ggtccatcgt caggacctcg gcctcgccct cgcccagtgc
      601 gtcccgtaac tgacgcggaa aaacgcccca gtggcgagcg tcgcggccga gtccgcgcag
      661 caataaccag tagttcaagc tttcaactcc tcgatacaga accggcgatt gttctggctc
      721 acgcctgcca atccgacgca gcttcaggtg ctcgatgtcg ccttgttgct acgaccagca
      781 gggctgtcgt tgattggcga gcgatcgtgg cagtctatcg gcgcctggcc atgagcgccc
      841 gaatcctcgc caactgcgcc tggcgaacac ggtgcaactt cgtcgtcttc ttcgacctgt
      901 ctcggatcgt ctcaatcatt gccggacttg tcggccttcc gacaaatctc tgtcgggtct
      961 gcgtcgcgcc gacttggtac ggccgatcaa gttcggcaag tgactgaacc ggcagaatta
     1021 attcagctgg cactgatcgt gcaaaggccg gcttgtcttc ctgcgagtgc ccgtcatggc
     1081 gaaggtccta cccggcaaga aagcctgcac cgtcaatccg ctcaagatga gcgcgccgat
     1141 cggcgccgcg cttgccttcc tcggcatgga tcgggcgctg ccactgctgc atggttcgca
     1201 aggatgcacg gctttcggat tggtgctctt cgtccgtcat tttcgcgaga cgattccgct
     1261 gcagacgacc gcgatgagcg aggtgacgac aatcctcggt ggcatggaca acgtcgaaca
     1321 ggcgttgctg aacatcaaga agcgtgccga tccggcattg atcggcattt gctcgaccgg
     1381 catcactgag gccaaggggg acgacgtcgc cggtttcctt acactgatcc gcgagcgtca
     1441 tcctgagctg gccggcaccg aggtcgtcta cgtgtcgacc cccgatttcg ttggcggctt
     1501 cgccgacggt tgggcaaaag ccgtgagcgc gatggttcgc gatttggttc cggcgactcc
     1561 ggtgcggacc ccctggcagg tgaacatcct tgccggttcg cacctcagcg tcggtgatat
     1621 cgaggagatc cgcgagaccg ttgaagcctt cgggctgacc ccgatcatcc tgcccgacct
     1681 ctcaggctcg ctcgacggcc atattcccga aaactttctg ccccatacgc tgggcggaac
     1741 gcggctggag gatgtgcgcc gcatgggcgc atcggcgatg accatcgcca tcggcgaatc
     1801 gatgcgcccg gctgccgacc tgctctggaa gcaggcgggc gttcccttcg aagtgctcga
     1861 tcgactgacc ggactggcgg cctgcgacgg tttcatccgc atgctttccg acctttccca
     1921 caacgaggtg ccgtcgcgct atcggcggca gcgcagccaa cttcaggatg cgatgctcga
     1981 cgggcacttc tatttcggcg ggcagaaggt cgcaatcggc gccgagccgg atctgctgta
     2041 cgcgctgtcg catttcttcg ccgacctggg tgccgaggtc gtctgcgccg tggcgccgca
     2101 ggatgcgccg attctcgagc gggtcgcggc cgagaccgtg caggttggcg atctcgagga
     2161 cttcgagcag ggcgcgaccg gagccggtct tctggtgacg cattcgcacg ggcgccaggc
     2221 agcggcgcgg ctcggaatcc cgttctatcg cgtcggcatt ccgaccttcg atcgtctcgg
     2281 tgccgcgcac cgctgcacgg tcggctaccg cggcacccga aatctgatct tcgaagtcgg
     2341 caacctcttt attgagcggc ttcacgccca cgagaacgcc gttgagacgg cggacgtcac
     2401 cggaaacgcc catgcgccat ctgtcgctgg tcactgaacc catcacagga gaacgggtca
     2461 tgaaagtcgc cttcgccacg gaggacctga agacggtcga tgcgcatttc ggctgggcca
     2521 ggcaactcgc tgtctatgaa atcggcccgg aatcgcatgc gctgctcgag gtcgtgcagt
     2581 tcgacggcga cctcaaggag gacggcagcg aggacaagct cgaacccaag ctcaacgcca
     2641 ttcgcgactg cgccatcgtc tacgtcgcgg cgatcggtgg ctcggcggcg gcgaaggtcg
     2701 tcaacctgcg cgtgcatccg atcaaggtca cgcagccgga agccatcgcc gacatcatcg
     2761 tcaagctgca gggcgtgctt cgcgggacgc cgccgccctg gttgcgcaag gttctactca
     2821 aggacaagcc gcgcagtttc gatttcgacg acgaggaaag cgaggcgcac catggctgaa
     2881 acgatggatc gcgaagcgac tgtcgtcgcg tcggcctttc tcggagaact ggtcaaggtg
     2941 ctgcgcgctc aggacagcta cggtcattgg gatggcaaat cggacgccga agtgctcgcg
     3001 ccctatgtca tcgacaagga agcgaggc
//
LOCUS       DATDAA010000273         3397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_125241,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000273 DATDAA010000000
VERSION     DATDAA010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125241"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VIS73_08590"
     CDS             complement(<1..210)
                     /locus_tag="VIS73_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3364 domain-containing protein"
                     /protein_id="HEY5763252.1"
                     /translation="MPQSAEKVLDHAGLFSEPEYKEIFARKAAEFEGGCGAAKVAEVA
                     EWTKTMEYREINFQRTALTINPAKAC"
     gene            complement(222..1667)
                     /gene="nifD"
                     /locus_tag="VIS73_08595"
     CDS             complement(222..1667)
                     /gene="nifD"
                     /locus_tag="VIS73_08595"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein alpha chain"
                     /protein_id="HEY5763253.1"
                     /translation="MSAVLDTKTRNQALINEVLEVYPEKAKKKRAKHLNVYEEGKPDC
                     GVKSNIKSLPGVMTIRGCAYAGSKGVVWGPIKDMVHISHGPVGCGHYSWATRRNYYNG
                     TTGVDAFGALQVTSDFQEKDIVFGGDKKLDKLIDEITQVFPLAKGISIQSECPIGLIG
                     DDIEAVSKKKSKEVNLPVVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKADYNAPFES
                     TPYDVVLMADYNIGGDAWSSRILLEEMGLRVIGQGTGDATLKEIEMMPRAKLLLLHCY
                     RSMNYIARHVEEKYGVPWFEYNLFGPTKIAESLRGIAAFFDDTIKAKAEEVIAKYTKL
                     SQEVIDKYKPRLAGKKVMLYVGGLRPRHVVGAYEDLGMEVVGTGYEFAHGDDYQRTTK
                     YVKDGTLIYDDVTGFEFEKFAEAIRPDLVGSGVKEKYIFQKMGIPFRQMHSWDYSGPY
                     HGYDGFAIFARDMDMAINAPVWSMTKAPWKK"
     gene            complement(1692..2564)
                     /gene="nifH"
                     /locus_tag="VIS73_08600"
     CDS             complement(1692..2564)
                     /gene="nifH"
                     /locus_tag="VIS73_08600"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HEY5763254.1"
                     /translation="MSLRQIAFYGKGGIGKSTTSQNTLAALAEMGQKILIVGCDPKAD
                     STRLILHAKAQDTVLSLAADAGSVEDLELEDVLRVGYRDIRCVESGGPEPGVGCAGRG
                     VITAINFLEENGAYDGVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMYA
                     ANNISKGILKYANAGGVRLGGLVCNERKTDKEWELADALAKKLGTQLIHFVPRDNVVQ
                     HAELRRMTVLEYDPESKQAEEYRQLARKVHENKGNGVIPTPISMEELEDLLLEFGIMQ
                     KDDEAIVGKAKAAA"
     gene            complement(2782..>3397)
                     /locus_tag="VIS73_08605"
     CDS             complement(2782..>3397)
                     /locus_tag="VIS73_08605"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY5763255.1"
                     /translation="WQGVLVIAAADSEAALRAEAVFAGHLSAFDYAALSLIGRDAPLV
                     TMRLAHRQAPEADELELLLAAAGLGAAASDSDGPAVMEFADASRRVAKRARIEAQRLV
                     AFAFFGEVAAAGWLREAMLAGDPLDGYRRYLFAPLAQPPVARPSRGRVLCNCLDVAEA
                     DIAQAVADGADSLDVLKERLGAGTRCGGCLPELRRRFEQLAATV"
BASE COUNT          600 a         1085 c         1028 g          684 t
ORIGIN      
        1 gcaggccttg gctgggttga tcgtcaatgc agtccgctgg aaattgatct cgcggtactc
       61 catcgtcttg gtccactcgg caacttcggc gaccttggcg gcaccgcagc cgccttcgaa
      121 ctcggccgcc ttgcgcgcaa agatttcctt gtactcgggc tccgaaaaca gaccggcgtg
      181 gtcgagaacc ttttcagctg attgaggcat ggcaatgctc cttatttctt ccagggggct
      241 ttggtcatcg accagaccgg cgcgttgatc gccatgtcca tgtcgcgcgc gaaaatcgcg
      301 aagccgtcat aaccgtggta cgggcctgag tagtcccagc tgtgcatctg gcggaaggga
      361 atgcccatct tctggaagat gtacttctcc ttgacgcccg agcccaccag gtccggacgg
      421 atcgcctcgg cgaacttctc gaattcgaag ccggtcacgt cgtcgtagat cagcgtcccg
      481 tccttcacgt acttggtcgt gcgctggtag tcgtcgccgt gggcgaactc atagccggtc
      541 ccgacgacct ccataccgag gtcctcgtag gcgccgacga cgtggcgcgg acgcagtccg
      601 ccgacgtaga gcatcacctt cttgccggca agacgcggct tgtatttgtc gatgacttcc
      661 tgcgagagct tggtgtactt ggcgatcacc tcttccgcct tggccttgat cgtgtcgtca
      721 aagaaggcag cgatgccacg cagcgactcg gcgatcttgg tcggcccgaa caggttgtac
      781 tcgaaccaag gcacgccata cttctcttcg acgtggcgag cgatgtagtt catcgatcga
      841 tagcagtgca gcaacagcag cttggcgcgc ggcatcatct cgatttcctt gagcgtcgcg
      901 tcgccggtgc cttgaccgat cacgcgcagg cccatctctt cgagcaggat gcgcgaggac
      961 caggcatcgc caccgatgtt gtaatcagcc atcagcacga cgtcgtaggg ggtggattcg
     1021 aagggcgcgt tgtagtcggc cttgtcgaaa acccagtcac ggatcgcgtc gttggcaatg
     1081 tggtggccca acgactggga aaccccacgg aagccttcgc accggaccgg aacgactggc
     1141 agattcactt ccttcgactt cttcttcgac accgcctcga tgtcatcacc aatgaggccg
     1201 atcgggcact ccgactgaat cgagatgccc ttggccagcg ggaagacctg cgtgatctcg
     1261 tcgatcagct tgtcgagctt cttgtcgccg ccgaagacga tgtccttctc ctggaagtcg
     1321 gaggtcacct gcagcgcgcc gaaggcgtcg acaccggtcg tgccgttgta gtagttgcga
     1381 cgcgtcgccc acgagtagtg gccgcagcca actgggccat ggctgatgtg caccatgtcc
     1441 ttgatcgggc cccagaccac gcccttggaa ccggcgtagg cgcacccgcg gatcgtcatc
     1501 acgcccggca gggacttgat gttcgacttg acgccgcaat cgggcttgcc ctcttcgtac
     1561 acgttgaggt gcttggcgcg cttcttcttc gccttttcgg gataaacctc gagcacctcg
     1621 ttgatcagcg cctgattgcg cgttttcgta tccaataccg cactcatgtc gattctcctg
     1681 tgtgcgttga gttaggcggc tgccttggcc ttgccgacaa tggcctcgtc atccttctgc
     1741 ataattccga actcgagcag caggtcttcc agttcttcca tcgagatcgg ggttgggatg
     1801 acgccgttac ccttgttctc gtgaaccttg cgcgcaagct ggcggtactc ttcggcctgc
     1861 ttcgactccg ggtcgtactc gagcaccgtc atgcggcgca gttcggcgtg ctggacgacg
     1921 ttgtcgcgcg gcacgaagtg aatcagctgg gtaccgagct tcttcgccag cgcatcggcg
     1981 agttcccact ccttgtcggt cttgcgctcg ttgcagacca gaccgccaag gcgcacgccg
     2041 ccggcgttgg cgtacttgag gatgcccttc gagatgttgt tggcggcgta catggccatc
     2101 atctcgcctg acatgacgat gtagatctct tgcgccttgt tctcgcggat cggcatggcg
     2161 aaaccaccgc aaaccacgtc gccgagcacg tcgtaggaga cgtagtcgac gccgtcatat
     2221 gcgccgtttt cctcgaggaa gttaatcgcg gtgatgacgc cgcgaccagc acagccgacg
     2281 ccgggttccg gaccgcccga ctcgacgcaa cggatgtcgc ggtaaccgac gcgcagaacg
     2341 tcttcgagtt ccagatcctc gaccgaaccg gcatcggcgg cgagcgacag caccgtgtcc
     2401 tgcgccttgg cgtgcaggat caggcgggtc gagtcggcct tcgggtcgca gcctacgatc
     2461 aggatcttct ggcccatctc ggccagcgca gcgagggtgt tctgggaggt ggtcgacttg
     2521 ccgataccac ctttgccata gaacgcaatc tgacgaagtg acattacatt tctccttgaa
     2581 gagtgaccta catttcacat ccggcaagcc gggacggtct ctctttcgca aggggtatgc
     2641 cagctcggtt tgatcggctc gtaacgacct tccagcaagg cttttcggcg ctttcaaccg
     2701 gcgcgaaata cctgtcgcga acacgacaat gtccgccgcc gggcggccgg gcccggacaa
     2761 agtgaagcaa gtggcggcgg ttcagacggt cgcggcgagt tgttcgaagc ggcgtcgcaa
     2821 ctccggcagg caaccgccgc accgggtgcc ggcgcctagc ctttccttca gcacatcgag
     2881 gctgtcggcg ccgtcggcga ccgcttgcgc gatgtcggcc tcggccacgt ccaggcagtt
     2941 gcagagaacc cggccgcgcg acgggcgcgc caccggtggt tgcgccagcg gcgcaaatag
     3001 gtaacgtcgg tagccgtcca gtggatcacc agcaagcatc gcctcgcgca accagccggc
     3061 cgccgcgacc tcgccgaaaa aggcgaaggc gaccagacgt tgggcctcga tccgcgctcg
     3121 cttggcgacc cgccgcgacg catcggcgaa ttccatcacc gccggcccgt cggaatcgct
     3181 ggccgccgca ccaagccctg cggcggccag cagcaactca agttcgtcgg cttcaggagc
     3241 ttgccgatgc gccaggcgca tcgtcaccag cggcgcatcg cgtccgatga gcgacaaggc
     3301 agcgtagtcg aaggcggaga ggtgccctgc gaacactgct tcggcccgca gcgccgcttc
     3361 ggagtcggcg gccgcgatga caagcacgcc ctgccag
//
LOCUS       DATDAA010000274         5924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_195007,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000274 DATDAA010000000
VERSION     DATDAA010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195007"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VIS73_08610"
     CDS             <1..689
                     /locus_tag="VIS73_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5763256.1"
                     /translation="IAASTAKRKLILVDAVFSMDGDCSDLVALLDIARRYDAWLVIDD
                     AHGFGVRGRNGRGSLDAERIGSDERIVYVGTLGKAAGLSGAFVAADETVVDWLIQSSR
                     THIFTTAAIPALSAAIVESIGCIRAGDDRRAQLEQLKERLRTGCAGLKWPLLESTTPI
                     QPLLIGDSHDALRLAEGLRQRGFWVPAIRPPTVPAGTARLRISLSATHSLEQVDALVA
                     ALHDLAKGAE"
     gene            686..1465
                     /locus_tag="VIS73_08615"
     CDS             686..1465
                     /locus_tag="VIS73_08615"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5763257.1"
                     /translation="MNSLGVTKLGQGPDLVFLHGWGFDSRVWQPVAEALSDSFSVRCI
                     DLPGYGASRDAPITSFDESVAAVLKQIPAGATLVGWSLGGMVALACARREPARLGGLV
                     LVATTPSFVRRDGWTQGMPPLMLGGFKVALRADLLGLLQHFAGLMNHGDQNARDLTRL
                     FKLLGKTDEPIDEALLLGLDWLRDIDLRDALPAIATPTLLIHGDADPLMPIDAAQAMA
                     EALPQARLETFPGVAHVPFFGDPARFVQCLRDFARSPAPAC"
     gene            1459..2223
                     /locus_tag="VIS73_08620"
     CDS             1459..2223
                     /locus_tag="VIS73_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5763258.1"
                     /translation="MLNRPVQAAFNRAAEHYDAVATTQSVVAARLFELLQLQRLPAPR
                     RILDAGCGTGGALVSFREAWPASETVALDFAPAMLAAAGKSAVSVCGNLERLPIRNCS
                     IDLYWSSLAIQWCELSRAVAEAVRVLRPGGTLAVTTLSVLTFAELRAAFAGIDSYGHT
                     LEFLEPDAIEAEIRRGGFAQLSRSRLMVTEHHQDLRTLLRRVKATGANQISGRRRPGL
                     LGRHAFAEAERRYEAFRAAEGLPLPYDVVMIIARRS"
     gene            2220..2909
                     /gene="bioD"
                     /locus_tag="VIS73_08625"
     CDS             2220..2909
                     /gene="bioD"
                     /locus_tag="VIS73_08625"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEY5763259.1"
                     /translation="MTAGVFVTGTDTGVGKTLVASALLRAACQAGLRAVGMKPVAAGT
                     DALGRQEDVEALMAAGNVVVERSAINPYCLAPPIAPHIAADEAGVMIEPERIRTACEA
                     LRARADLVVVEGAGGILVPLGPGLDTADLAKQLELPVLVVVGLRLGCINHALMTMEAA
                     ASRGLRITGWIANCVDPEMARLPQNVDSLRARINYPLLAQLDWRVNDDAAATAMKATL
                     DALLAPNHSQN"
     gene            2919..3524
                     /locus_tag="VIS73_08630"
     CDS             2919..3524
                     /locus_tag="VIS73_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEY5763260.1"
                     /translation="MKFLFDLFPVILFFAVFKIAGHYPADSQALASQLLGTGVLEAQA
                     PILMATAVAILATFAQISWVWTRHRKVDTMLWVSLAVITVFGGATLLFQNETFIKWKP
                     TVLYWLFAAILLGSSLFFRKNLMHKLLSEKIALTDAGWRTINLSWIGFFTAMGVINLV
                     VAYRFSTDTWVNFKLFGGMGLTLVFVLIQGLMLARHIVEEK"
     gene            3526..3828
                     /locus_tag="VIS73_08635"
     CDS             3526..3828
                     /locus_tag="VIS73_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY5763261.1"
                     /translation="MWYAIVAEDVANSLERRLAARPAHVERLKALVDAGRLLVAGPFP
                     AIDGADPGPAGFTGSLIVAEFASLTEARQWADADPYIAAGVYERVTVKPFKKVLPA"
     gene            3825..4088
                     /locus_tag="VIS73_08640"
     CDS             3825..4088
                     /locus_tag="VIS73_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEY5763262.1"
                     /translation="MSVETSLRERLAALEPTKLEIADDTEKHAGHAGAAGGGRHFRLT
                     IVSGHFTGKPLLARHRLVIQAAGELMQGAIHALSINAKAPGEV"
     gene            4153..4947
                     /locus_tag="VIS73_08645"
     CDS             4153..4947
                     /locus_tag="VIS73_08645"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5763263.1"
                     /translation="MTLNSLRFSAVAVLLALSTGTQALAAADKPLATVNGKAISQSYA
                     DVFVREQLAKGVADSPELRNAVREELIRREALVQAARAKGVDKSPEVKARVEFSQQEV
                     LIGAFIQKHLSDNPISDEKAKAEYEKMKAGMTAREYKARHILVESEADAKAIIAKLDG
                     GAKFEDLAKESKDPGSKANGGDLGWNQPSAFVKPFSDAMVALEKGKYTQSPVQTDFGY
                     HVILTEDSRTSEPPAFEQIKPQLLQHLQQQEVGKLIAQIREKAKVK"
     gene            complement(5092..5316)
                     /locus_tag="VIS73_08650"
     CDS             complement(5092..5316)
                     /locus_tag="VIS73_08650"
                     /inference="COORDINATES: protein motif:HMM:NF018369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HEY5763264.1"
                     /translation="MKRVLLAFAVSLLAMPAFAQIKPCEELKAEIAAKIEANGVTTYQ
                     LEIIPIDQETDWKVVGTCDGQTKKIVYRRG"
BASE COUNT         1077 a         1961 c         1791 g         1095 t
ORIGIN      
        1 tgatcgccgc gtcaacggca aaacgcaagc tgatcctcgt tgacgccgtg ttcagcatgg
       61 atggcgactg ctctgatctg gttgccttgc tcgacatcgc ccgccggtac gacgcctggc
      121 tggtcattga cgacgcccac ggcttcgggg tccggggccg caacggccgc ggcagccttg
      181 acgctgaacg aatcggaagc gacgaacgga tcgtctacgt cggcacattg ggcaaggctg
      241 ccgggctatc aggcgccttc gtggcagccg acgagacggt cgtcgactgg ctgatccagt
      301 cgtcgcgaac ccacatcttc acgacggcgg caatcccggc cctgtcggcg gccatcgtcg
      361 aaagcatcgg ctgcatccgt gccggcgacg accgccgcgc ccagctcgaa cagctgaagg
      421 aacgccttcg caccggctgt gccggactga aatggcccct cctcgaatca acaacgccga
      481 tccagccgct gctgatcggc gacagtcacg atgcgcttcg cctcgccgag ggcctgcgcc
      541 agcgcggctt ctgggtacct gcgatccggc cgccaacggt gccagccggc acggcacgac
      601 tgcgcatttc gctgtcggca acgcactcgc tggagcaggt tgatgccctg gtcgccgccc
      661 tgcacgatct ggcaaagggt gcagaatgaa ctcgctcggc gtcaccaagc tggggcaagg
      721 gcccgacctc gtctttctgc acggctgggg gtttgactcg cgcgtctggc agccggttgc
      781 cgaagcgctg agcgactcgt tctcggtgcg ctgcatcgac ctgcccggct atggcgccag
      841 ccgcgacgca ccaatcacat catttgacga atcggtcgcc gccgtcctca aacagatccc
      901 cgctggcgca acgctggtcg gctggtcgct cggcggcatg gtcgcccttg cctgcgccag
      961 gcgcgagccc gctcgcctcg gcggtctggt cctggtcgcg acaacaccga gcttcgtcag
     1021 gcgcgacggc tggacccagg gcatgccgcc gctgatgctc ggcggtttca aagtagcgct
     1081 gcgggccgat ctcctcggcc tgctgcagca ttttgcgggc ctcatgaacc atggcgacca
     1141 gaacgcacgt gacttgaccc gtctattcaa gctgctggga aaaacagacg agccgatcga
     1201 cgaggccttg ctgctggggc tggactggct gcgagatatt gacctgcgcg atgccttgcc
     1261 ggcgatagcc acccccacgc tgctaattca cggcgacgca gatccgctga tgccaatcga
     1321 tgctgcgcag gcaatggccg aagccctccc gcaggcgcgc ctcgaaacct ttccgggggt
     1381 cgcccacgtg cctttcttcg gcgaccctgc gcgttttgtc caatgcctgc gcgacttcgc
     1441 gcgctcgccg gctcccgcat gctgaaccgt ccggttcagg ccgccttcaa ccgcgccgcc
     1501 gaacactatg acgccgtcgc gacgacacag agcgtggtgg cggcgcgcct gttcgagttg
     1561 ctgcaactgc aacgattgcc agcgccgcgc cgcatccttg atgccggttg tggcacaggc
     1621 ggagccctcg tttcgtttcg cgaggcctgg cctgccagtg aaaccgtggc gctcgatttc
     1681 gccccggcca tgctcgccgc cgcaggcaag tcggccgtct ccgtctgcgg caacctggag
     1741 cggctgccga tccgtaattg ctcgatcgat ctctactggt cgagccttgc catccagtgg
     1801 tgcgagctgt cgcgagcggt cgccgaggcg gtgcgagtgc tgcggccggg cggcaccctc
     1861 gccgtgacga cgctgagcgt gcttaccttt gccgagttgc gtgcggcctt cgccggcatc
     1921 gatagctatg gccacacact ggagttcctc gaacccgacg ccatcgaggc cgaaatacgg
     1981 cgcggcggat ttgcccagct gagccgtagc cggctcatgg tgaccgagca ccaccaggac
     2041 ttgcgcacgc tgctgcgccg ggtcaaggcg accggcgcca atcagatcag cggccggcgt
     2101 cgcccgggac ttctcgggcg gcacgccttc gccgaagccg agcggcgcta tgaggctttt
     2161 cgcgccgccg aggggctgcc actaccttac gatgtcgtga tgatcatcgc gaggcgttcg
     2221 tgactgccgg ggtattcgtc accggcaccg acaccggcgt tggcaagacg ctggtggcgt
     2281 ccgccctgct acgcgcggcg tgccaggccg gactgcgcgc cgtcggcatg aagccagtgg
     2341 cggccggaac cgatgcgctg gggcggcagg aagacgtaga ggcactgatg gccgccggca
     2401 acgtcgtcgt cgagcgctcc gcgatcaacc cctattgcct ggcgccgccg atcgcaccgc
     2461 acatcgccgc cgatgaggcc ggcgtgatga tcgagcctga acggattcgc accgcctgcg
     2521 aggcacttcg cgcccgagcg gacctcgtcg tcgtcgaggg cgccggcggc attctcgtgc
     2581 cgcttggacc gggactcgat acggccgatc tcgccaaaca gcttgagttg ccggtcctgg
     2641 tcgtcgtcgg cctacgcctg ggctgcatca accacgccct gatgaccatg gaagccgccg
     2701 ccagccgcgg tcttcgcatc actggctgga tcgccaactg tgtcgatccg gaaatggcgc
     2761 gcctgccgca gaatgtcgat tcgctacggg cgcgaattaa ctatccgcta ctcgcccaac
     2821 tcgactggcg ggtcaacgac gacgcggcag cgaccgcaat gaaggccacg cttgacgccc
     2881 tgctcgcccc aaaccattcc caaaactgag ttgccaacat gaaattcctc ttcgacctct
     2941 ttccggtcat tctcttcttc gccgtcttca agattgccgg ccactatccg gctgactcgc
     3001 aggcactggc gtcgcagctg ctgggcaccg gggtgctgga ggcacaggcg cctatcctca
     3061 tggccaccgc ggtggcaatt ctggcaacct tcgcccagat cagctgggtc tggacccgcc
     3121 atcgcaaggt cgacaccatg ctttgggtca gccttgccgt catcacggtc tttggtggcg
     3181 caaccctgct gtttcagaac gagaccttca tcaagtggaa gccgacggtc ctctattggc
     3241 tgttcgcggc gatcctgctt ggatcttcgc tgttcttccg caagaacctc atgcataaac
     3301 tcctgtccga aaagatcgcg ctgaccgacg ccggctggcg gacgatcaac ctgtcgtgga
     3361 tcggcttctt cacggcgatg ggtgtcatca acctggtcgt cgcgtaccgc ttttccaccg
     3421 acacctgggt caatttcaag ttgttcggcg gcatgggcct gacgctggtc ttcgtcctga
     3481 tccagggcct gatgctggcc cgccatatcg ttgaggagaa atagcatgtg gtatgcaatc
     3541 gttgccgaag acgttgccaa ttcgctcgaa cgacggctgg cggcaaggcc ggcgcatgtc
     3601 gagcggctga aagcgctagt cgatgccggg cgcctgctgg tcgcaggccc attcccagca
     3661 atcgatggtg ccgaccccgg accggccgga tttaccggaa gcctgatcgt cgccgagttc
     3721 gcgtcgctca ccgaggccag gcagtgggcc gacgccgacc cctacatcgc tgccggtgtc
     3781 tacgagcggg ttaccgtcaa gccgttcaag aaggtcctgc ccgcgtgagc gtcgaaacca
     3841 gtttgcgcga acggctcgcg gcgctcgaac ccacgaaatt ggaaatcgcc gacgacaccg
     3901 aaaaacacgc cgggcatgct ggcgccgctg gcggcggtcg tcatttcagg ctgaccatcg
     3961 tttccgggca tttcaccgga aaaccgcttc tagcgagaca tcggctcgtc atccaggctg
     4021 ctggcgagct gatgcagggt gcaattcatg cgttgagcat caacgccaag gcgcccggtg
     4081 aagtatgatt cggcggcgcg gtcgagactg actgccagct cttaatcttc cctgactacc
     4141 aatggaatgt tcatgacctt gaattcgcta cgcttttccg ccgttgccgt actgctcgcg
     4201 ttgagcaccg gcacccaggc ccttgcggca gccgacaaac cgctagcaac cgtcaatggc
     4261 aaggccattt cgcaaagcta cgctgacgtc ttcgttcgcg agcaactcgc caagggcgtc
     4321 gccgacagcc ccgagctgcg caacgcagtc cgcgaggaac tgatccgtcg cgaggcgctg
     4381 gtacaggctg cgcgcgccaa gggcgtcgac aagtcgccgg aagtcaaggc gcgggtcgag
     4441 ttctcgcaac aggaagtgct gatcggcgcc ttcatccaga agcacctgag cgacaacccg
     4501 atttccgacg agaaggccaa ggccgagtac gaaaagatga aggccggcat gaccgcccgt
     4561 gagtacaagg cgcggcacat cctcgtcgag tcggaagccg acgccaaggc gatcattgcc
     4621 aagctcgacg gcggcgccaa gttcgaggac ctggccaagg agtccaagga tccgggttcg
     4681 aaggctaatg gcggcgatct tggctggaat cagcccagtg ccttcgtcaa gcccttctcc
     4741 gacgcgatgg tcgcgctgga aaaaggcaag tacacccagt caccggtgca gaccgatttt
     4801 ggctaccacg tgatcctgac cgaagacagc cggacctccg agccgccggc gttcgagcag
     4861 atcaagccgc aactgctcca gcacctccag cagcaggaag ttggcaagct gatcgcacag
     4921 atccgcgaaa aggccaaggt caagtaagcg tgaatgccgg aggcaggccc tgcctgggcc
     4981 cgcccccggt acctacccgc cggccggccc ggcgcggcac cagagaccga ccgcgctcca
     5041 ggtcatcgct atccacacct ctttaggcta ccaacagccg tcgctcgttt gtcagccgcg
     5101 acggtagacg atcttcttgg tttggccgtc gcaggttccg acaactttcc agtccgtttc
     5161 ctgatcgatc gggatgatct cgagctgata ggtggtcacg ccgttggctt cgatcttcgc
     5221 ggcgatctcg gccttcaatt cctcgcaggg tttgatctgc gcgaaagcag gcatagccag
     5281 cagcgaaacg gcgaaagcaa gtagtacgcg tttcatggga tctcctcaaa aggggtatga
     5341 caggggaaga ccattctagt cgcgctaccg catttggaac gatgccgcga cgagcacttc
     5401 gagagacaag ttcgccggtt gtcgcgatca acttgatctt tcccattttc ttttacgatt
     5461 agtgaattct aacgccccta ttgaaaggtg cgccgcaagc gggtctcggc gagatccgcc
     5521 tattgcaacg ctggaaatca aactaatcaa aaactccgca gcgccgctag aaacctgaat
     5581 tgtgagtcgt tccccggaaa acgactggcc tcaccgccag ccgaaatcac cagagccact
     5641 tccccgcgcc gccgaatggc cgccgcaaga gcagcgcatc aaagccggaa aattattttg
     5701 gtttaccaat tgtgaatctt gctgcattgc actatattga gcatcgctcg gtgcactgca
     5761 cggggcccta ctaacctaaa ggacattgaa gacatgaagc cgtacctcct cgccgccgcc
     5821 ctcgtcgttt tcgcggtttc cgcctgtggc aagaaagaag aagccgcccc ggttgctgcg
     5881 cccgcacccg ttgtcgctcc cgctcccgct cccgctccgg ctga
//
LOCUS       DATDAA010000275        11088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_370180,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000275 DATDAA010000000
VERSION     DATDAA010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11088
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370180"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VIS73_08655"
     CDS             complement(<1..355)
                     /locus_tag="VIS73_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY5763265.1"
                     /translation="MIVQLFRRGWSALAAHIDAPLFAVAMILALIGLASVYSASVDVP
                     ARVNAQLANMAIAFAAMWIIARVPPRHMARVAVFIYVVGLLLLLGVWLFGVKVNGARR
                     WLDLGVGRIQPSEFMK"
     gene            complement(352..2241)
                     /gene="mrdA"
                     /locus_tag="VIS73_08660"
     CDS             complement(352..2241)
                     /gene="mrdA"
                     /locus_tag="VIS73_08660"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY5763266.1"
                     /translation="MAGFTFADEEAWELRQFRQRAVVAGLVVLVAFVILFGRFVYLQV
                     FRHDYYLTRAQENRISIVPVVPQRGMIVDRNGTVLARNDSAFTLEITPGRTAGLEQTI
                     DLVAQLIEIDSRDRRRFRKLLEESKNFESLPLKVRLSDEEVARVSAQLYRLPGVEVKA
                     RQFRQYPLGGLASHALGHMGRISERDRDFLVASDSVANYRGTDHIGKLGLENAYELDL
                     HGTTGYQEVETDAAGRAVRVLSRNAPTPGNDLTLTIDAELQAVAEAAIGERRGAAVAL
                     DPNSGAILALVSTPTFDPNLFVDGIGIQEWDELNTSEDRPMLNRALAGTYPPGSTFKP
                     YMALAALTLGKRTPQQAIDDRGLFVFGNHVFRDDKVGGHGMVDMHKSIVESCNTYYYI
                     LANDLGIDAIDSFMAPFGFGQLSGIDIPGELEGVLPSQEWKRKRFRTPGQQRWYAGET
                     ISVGIGQGYNAYTPLQLALAMATLANGGTVYRPHLVAAIGDRADGTAVPVVPEVVRKL
                     PLKEEHVAVIHRALIDVNRDGTGRWAFAKAPYTAAGKTGTAQVYSLRGEKYDEKTAAD
                     TLKDHSLFIAFAPAEAPTIAVAVVVENAGFGATSAAPAARKIIDQYLLAGKADATGGN
                     KVALP"
     gene            complement(2302..2826)
                     /gene="mreD"
                     /locus_tag="VIS73_08665"
     CDS             complement(2302..2826)
                     /gene="mreD"
                     /locus_tag="VIS73_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY5763267.1"
                     /translation="MMHIGSPGSRILLPARGWFIALSIAIGFVLDLLPMGYSPTLPDF
                     SALILCFWAIREPRRVGMGIALILGIFADVAAGSVMGQHALAYVALAYLCGTVSRRVV
                     WFDLVSQAAHVWVLLLIATCIRIASGLVGGGEFPGWAFLIGPLIGAVLWIPMNYLLLL
                     PQYQPIERDDNRPI"
     gene            complement(2823..3746)
                     /gene="mreC"
                     /locus_tag="VIS73_08670"
     CDS             complement(2823..3746)
                     /gene="mreC"
                     /locus_tag="VIS73_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY5763268.1"
                     /translation="MDRQPPPFFKRGPAPLAQLSFYVALSIALMIVDLRFHYLAVVRD
                     VVAAVVQPLQIIAQVPVRGWLSVHEYFAATSELQAEKALLREAREAVGPQLLRVEQLE
                     TENERLRRLLELKAREPAAGATVRVLHATRDPFIHRIIIDRGLGQGVIPGQGVMDEAG
                     LIGQVTRVLPLSSEVTLLTDKTQATSVQLVRTGLRTVAFGAGRGLFELRYLPAGADIE
                     VGDLLVSSGLDGVYLPGLPVARVTVVSRENEYAFARVLAIPVSGIEHFGEVVLLQPPA
                     PIAEPHPEFGKTADELLPAPAKSRRRQGVKP"
     gene            complement(3818..4861)
                     /locus_tag="VIS73_08675"
     CDS             complement(3818..4861)
                     /locus_tag="VIS73_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY5763269.1"
                     /translation="MFGYLRSYFSNDLAIDLGTANTLIYLRSKGIVLDEPSVVAIRTE
                     GGPHAKKTIQAVGRQAKEMLGKAPGQITVIRPMKDGVIADFTVTEQMLKQFIKKVHDS
                     KLFSPSPRIIICVPCGSTQVERRAIRESAIGAGASQVYLIEEPMAAAIGAGLPVADAT
                     GSMVVDIGGGTTEVGVISLGGMVYSGSVRVGGDKFDEAIINYIRRNYGMLIGEITAEN
                     IKKEIGSAFPGSEVREMEVKGRNLAEGIPRSFTISSNEILEALTEPLNQIVSAVKSAL
                     EQTPPELGADIAEKGMVLTGGGGLLRDLDRLLMEETGLPVIVAEDPLTCVVRGSGIAL
                     EKMDKLTSIFASE"
     gene            5061..5348
                     /gene="gatC"
                     /locus_tag="VIS73_08680"
     CDS             5061..5348
                     /gene="gatC"
                     /locus_tag="VIS73_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004574467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY5763270.1"
                     /translation="MSLELDQVKRIAALARIAIGDDEAQAVRDQLNRILAMVETMQAV
                     DTTGIEPMAHAQDLSQRLREDRVSESDRRSDFQRLAPEAEAGLYLVPKVIE"
     gene            5405..6877
                     /gene="gatA"
                     /locus_tag="VIS73_08685"
     CDS             5405..6877
                     /gene="gatA"
                     /locus_tag="VIS73_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY5763271.1"
                     /translation="MTDASLSELRAQLDARKVSSRELTQACLERIAARNAELNAFIST
                     DAEKSLTLADAADRRIAAGEAGPLTGIPLAHKDIFCAKGWRTSCGSKMLADFVSPYDS
                     TVVARCDAAGAVNLGKLNMDEFAMGSSNENSHFGPARNPWDLNRVPGGSSGGSAVAVA
                     AGLVSAATGTDTGGSIRQPAAWCGLTGLKPTYGAVSRWGMVAYASSLDQGGPIARSAA
                     DCAVLLNAIAGFDEKDSTSVERPAEDYARALAAPAERRLKGLRIGLPTAFFGPGIAPG
                     VRAAVDAAIAQFRELGAEIREVDLPNASLSLPAYYVIALAEASSNLSRFDGVRYGYRA
                     SEYGDLADMYAKSRAQGFGAEVKRRILVGTYVLSHGYYDAYYLQAQKIRRLIADDFRR
                     TFAECDLIAGPTTPTTAFRIGEKSDDPVQMYLDDIFTVPVNLAGLPAMSIPCGMADGL
                     PVGLQLIGDYFSEARLIGAGDAYQQATDWHQRRPGGEARK"
     gene            6874..8319
                     /gene="gatB"
                     /locus_tag="VIS73_08690"
     CDS             6874..8319
                     /gene="gatB"
                     /locus_tag="VIS73_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY5763272.1"
                     /translation="MSLGNWEVVIGIETHAQLQTHTKIFSGASTDFGAEPNVQACLVD
                     LAYPGVLPVLNRQAVECAIRFGLAVGAQVAPRSVFARKNYFYPDLPKGYQISQFELPV
                     VQGGALTILSGDSEKTIRLTRAHLEEDAGKSLHEDFHGKTGIDLNRAGTPLLEIVSEP
                     DLRSSAEAVAYARTLHALVRWIGICDGNMQEGSFRCDANVSVRRPGAPLGTRREIKNL
                     NSFRFMQQAIDYEVRWQIETIEDGGRIEQATVLFDPDSGETRAMRSKEDAHDYRYFPD
                     PDLLPLLIADDWVSRVRATLPELPNAKRARFVADYGLSTYDATSLTASLDTADYYEAV
                     VAIAGKAQAKPAANWVMVDLAARLNKEGLEIAASPVSAAQLGGLVARIGDGTISNSIA
                     KKVFEALWAGEGAGADEVIEKQGLKQITDTGAIEAMVDAVIAANPQNVAEFRAGKEKA
                     FNALVGQVMKAAKGKANPQQVNELLRRKLAE"
     gene            complement(8368..8967)
                     /locus_tag="VIS73_08695"
     CDS             complement(8368..8967)
                     /locus_tag="VIS73_08695"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY5763273.1"
                     /translation="MLLALSAAPAIAQNLYCCKDNSGRKVCGDRLPTQCVGRPHTVRG
                     PGGYTTKVEGFISPSERKAREAEERRRREAEEAAAEQLRLDRVLLSTYGNVRDIDIAH
                     ERAREDVTKAIEEAERRIDVANKRRAKYQNEAEFYKKKAIPPEIKRGLRDTEIEIRSQ
                     TDLIAVKRKEFEGMAARFADERERYLNLTVRGRSERLER"
     gene            complement(8987..9628)
                     /gene="pyrE"
                     /locus_tag="VIS73_08700"
     CDS             complement(8987..9628)
                     /gene="pyrE"
                     /locus_tag="VIS73_08700"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY5763274.1"
                     /translation="MRVADDFIAFSVAQQVLRFGEYKTKAGRLSPYFFNAGLFNDGAS
                     LDRLAQFYARTILASGVAFDMLFGPAYKGIALAAGTAMALAREGRNLPFAFNRKEAKD
                     HGEGGMLIGAPLKGRVLIIDDVISAGTSVRESVELIRAQGATPAGVVIALDRMERGQG
                     ELSAVQEVERDYRIPVISVASLTDLMSFLDARGDLAQHRSAVAQYRSTYGVAA"
     gene            9669..10451
                     /locus_tag="VIS73_08705"
     CDS             9669..10451
                     /locus_tag="VIS73_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY5763275.1"
                     /translation="MLRIITLNLNGIRAAWRKGFHDWLAAQGASVVCLQELKARDADL
                     SAEMRAAAGLSGHFLHAEQAGYSGVGLYLDRPPAALATGIDDAEFDAEGRVLRADFDD
                     FSIVSLYLPSGSSSEERQAAKFRFMARFVTVMDEWRRSGREFIICGDWNIAHREIDLK
                     NWKSNQKNSGFLPEERAWLSGVFEEQGWVDVYRQLHPEATDACYTWWSNRGQAWAKNV
                     GWRLDYQIATPGIAACARTAEIYKAQRFSDHAPLIIDYDWPA"
     gene            complement(10433..>11088)
                     /locus_tag="VIS73_08710"
     CDS             complement(10433..>11088)
                     /locus_tag="VIS73_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY5763276.1"
                     /translation="LVSRIPGRDVEQPAAAHCTAIGDWLAAMHAAQSDFVARWPNPRG
                     VVWRSTAAAEVRQWLPAEQHRLLDEALAADAQLAALDLPRGLVHADLFRDNALFADAD
                     HAVLGGVIDFYFAGEDALLFDLAVVANDWCVVAGLPDAAREDALLAAYGRRRPLTAAE
                     RRAWPAMRQVAALRFWLSRLLDAQAPRSGTMVLIKDPDEYARLLALLSGAAAQAGQS"
BASE COUNT         1900 a         3564 c         3725 g         1899 t
ORIGIN      
        1 gcttcatgaa ttccgatggc tggattcgcc cgacgcctag gtcgagccag cggcgggcgc
       61 cgttgacctt gaccccgaac agccagacgc cgagcagcag cagcaggcca acgacgtaga
      121 tgaagaccgc gacccgcgcc atgtgccgcg gcggcacgcg ggcgatgatc cacatcgcgg
      181 cgaaagcgat tgccatgttg gccagctggg cgttgacacg cgccggcacg tcgaccgagg
      241 cgctgtacac cgaggcgagg ccgattagcg ccaggatcat cgccaccgcg aatagcggtg
      301 cgtcgatatg ggccgcgagt gcgctccagc cgcggcggaa gagttggacg atcatggcag
      361 tgccaccttg ttgccgccgg tggcgtccgc ctttccggcc agcaggtact ggtcgatgat
      421 cttgcgcgcc gccggggcgg cagacgtggc gccaaaaccg gcgttctcga cgacgacggc
      481 aacggcgatc gtcggcgcct cggccggcgc gaaggcgatg aacagcgagt ggtccttgag
      541 tgtatcggcc gcggtctttt cgtcgtattt ctcgccgcgc aggctgtaga cctgcgccgt
      601 gccggtcttt ccggccgccg tatagggcgc cttggcgaag gcccagcggc cggttccgtc
      661 gcggttgacg tcaatcaagg cgcggtgaat gacggcaacg tgctcttcct tcagcggcag
      721 cttgcgcacg acttcgggca caaccgggac cgccgtcccg tcggcgcggt cgccgatcgc
      781 agcaaccaga tgcggccggt agacggtgcc gccattggcc agcgttgcca ttgccagcgc
      841 cagttgcagc ggcgtgtaag cgttgtagcc ttggccgatg ccaaccgaaa tcgtctcgcc
      901 ggcataccag cgttgctgcc ccggggtacg gaaacgcttg cgtttccatt cctgcgaagg
      961 cagcacgcct tccagttcgc cgggtatgtc gatgccggaa agctggccaa agccgaaagg
     1021 ggccatgaag ctgtcgattg cgtcgatgcc aaggtcattg gcaaggatgt agtagtaggt
     1081 gttgcacgat tcgacgatcg acttgtgcat gtcgaccatg ccgtggccgc cgaccttgtc
     1141 gtcacggaag acgtgattgc cgaagacgaa cagcccgcgg tcgtcgatcg cctgctgcgg
     1201 cgtgcgcttg ccgagcgtca gcgcggcgag cgccatatag ggcttgaagg tcgaccccgg
     1261 cggataagtg ccggcgagcg cgcgattgag catcggtcgg tcttccgagg tgttcagttc
     1321 gtcccattcc tggatgccga tgccgtcgac gaagagattc gggtcgaagg tcggcgtcga
     1381 taccagggcg aggatggcgc cgctgttagg atcgagcgcg accgcggcgc cgcggcgctc
     1441 gccgatcgcg gcttcggcga cggcctgcaa ctcggcgtcg atggtcagcg tcaggtcgtt
     1501 tccaggggtc ggcgcgttgc gcgagagtac ccgcacggct cgaccggcag cgtcggtttc
     1561 cacctcctgg tagccggtgg tgccgtggag gtcgagttcg taggcgtttt ccaggccgag
     1621 cttgccgatg tggtcggtgc cgcggtagtt ggcgaccgaa tcgctggcga cgaggaagtc
     1681 gcggtcacgc tccgagatcc gccccatgtg gccgagggcg tgcgaggcga gtccgccgag
     1741 cgggtactgg cggaactggc gggccttgac ctcaacgccg ggcagccggt aaagctgcgc
     1801 cgagacgcgc gcgacctctt catcggagag ccgtaccttt agcggcagag actcgaagtt
     1861 cttcgattcc tcgagcagct tgcgaaagcg gcggcgatcg cgcgaatcga tctcgatcag
     1921 ctgcgcgacg aggtctatcg tctgctcgag gccggcggtt cgccccgggg tgatctccag
     1981 agtgaaggcg ctgtcgttgc gcgccaggac cgtgccgttg cggtcgacga tcatgccgcg
     2041 ctgcgggacg accgggacga tcgagatccg gttctcctgc gcgcgcgtca ggtagtagtc
     2101 gtggcggaag acctgcagat agacgaagcg cccaaacagg atgacgaagg cgaccagcac
     2161 gacgagcccg gcgacgaccg cacgctggcg gaactggcgc agttcccagg cttcctcgtc
     2221 ggcaaaggtg aagccggcca tcgtcaggcg cgaccggtcg gctgcggcga gaccggcaat
     2281 cggatgtggc gcagcgttgc ctcagattgg ccggttgtca tcgcgctcga tcggctggta
     2341 ctgcggcagc agcagcaggt agttcattgg aatccacagt acggcgccga tcagcggccc
     2401 gatcaggaac gcccagccgg gaaattcgcc gccgccgacc aggccgctgg caatgcggat
     2461 gcaggtggcg atcagcagca gcacccagac gtgagccgcc tgcgagacca ggtcaaacca
     2521 gaccacgcgg cgcgacacgg tgccgcagag ataggcgagc gcgacgtagg ccagcgcgtg
     2581 ttggcccatg acgctgccag ccgcgacgtc ggcgaagatg ccgaggatca gtgcgatgcc
     2641 catgcccacc cggcgtggtt cgcgaatcgc ccagaagcag agaattagcg ccgagaagtc
     2701 gggcagcgtc ggcgaatagc ccatcggcag caggtcgagc acgaagccga tggcgatcga
     2761 cagggcaatg aaccagccgc gtgccggcag caagattctc gagcccggcg agccaatgtg
     2821 catcatggct tcactccctg gcgccgccgg ctcttcgccg gcgcaggtag caattcgtcg
     2881 gcggtcttgc cgaattcggg atggggctcg gcgatcggcg ccggcggctg aagcagcacg
     2941 acctcaccga aatgctcgat tcccgatacc ggaatcgcga gtacgcgcgc gaacgcgtac
     3001 tcgttttcgc gggaaacgac ggtgacgcgg gcgaccggca ggcccggcag atagacgccg
     3061 tcgagccccg aggaaaccag caggtcgccg acttcgatgt cggcaccggc aggtaggtag
     3121 cgtagttcga acaggccgcg gccggcgccg aaggctaccg tgcgcagtcc ggtgcggaca
     3181 agctggaccg aagttgcctg cgtcttatcg gtgagcagcg tcacttccga ggagagcggc
     3241 agaacacggg tcacctggcc gatcaggccg gcctcgtcca ttacgccctg gccgggaatg
     3301 acgccctggc ccagtccgcg gtcgatgatg atgcggtgga tgaagggatc acgcgtggcg
     3361 tgcaggacgc ggaccgtggc gccggcggcg ggttcgcgcg ccttgagttc gagcaggcgg
     3421 cgcagccgct cgttttcggt ctcgagctgt tcgacgcgca gcagctgcgg tccgaccgct
     3481 tcgcgggctt cgcgaagcaa ggccttctcg gcctgcagtt cgctggtcgc agcgaagtat
     3541 tcgtgaaccg acagccagcc gcgcacggga acctgggcaa tgatctgcag gggctggacg
     3601 accgccgcga cgacgtcgcg aacgaccgcc aggtagtgaa agcgcaggtc aacgatcatc
     3661 agcgcgatcg acagggccac gtagaagctg agctgggcca gcggcgcagg gccgcgcttg
     3721 aaaaacggag ggggttgccg gtccaaacgc ctagatcccg tgttgcagcc agttcatcgg
     3781 acgacgtccg gcgtagcgtc cgccacgccg gcgacgctca ctcgctggcg aaaatgctgg
     3841 tcagcttatc catcttctcg agcgcgatgc ccgagccgcg caccacgcag gtcagcgggt
     3901 cctcggcgac gatcaccggc aacccggttt cttccatcag caggcggtcg aggtcgcgca
     3961 gcaggccgcc gccgccggtg aggaccatgc ccttttcggc gatgtcggcg ccgagttcgg
     4021 gcggtgtctg ctcaagcgcg cttttcacgg ccgagacgat ctggttgagc ggctcggtca
     4081 gcgcctcgag gatctcgttg ctcgagatcg tgaagctgcg cgggatgcct tcggcgaggt
     4141 tgcggccctt gacttccatt tcgcgaacct cggagccggg gaaggccgaa ccgatttcct
     4201 tcttgatgtt ctcggcagtg atttcgccga tcagcatgcc gtagttgcgg cggatgtaat
     4261 tgatgatcgc ctcgtcgaac ttgtcgccgc cgacgcgaac cgatcccgag tacaccatgc
     4321 cgccaagcga aatgacgccg acctcggtgg tgccgccgcc aatgtcgaca accatcgatc
     4381 cggtggcgtc tgccaccggc agcccggcgc cgatcgcggc ggccatcggc tcttcgatca
     4441 ggtagacctg cgacgcgccg gcgccaatcg ccgactcgcg aattgcacgg cgctcgacct
     4501 gggtcgatcc gcagggcacg cagatgatga tgcgcggact cggcgagaac agcttcgagt
     4561 cgtgcacctt cttgatgaac tgcttgagca tctgctcggt gacggtgaag tcggcaatca
     4621 cgccgtcctt catcgggcgg atgactgtga tctgccccgg tgccttgccg agcatttcct
     4681 tggcctgccg accgaccgcc tgaatggtct tcttggcatg cggcccaccc tcggtgcgga
     4741 tggccactac cgacggctcg tcgaggacga tacccttgct gcggaggtag atcagcgtgt
     4801 tggcggtacc gaggtcgatt gccagatcgt tggaaaagta actgcgcaga tagccgaaca
     4861 tgtgcgaatg gtcccgagcg aatcgattgg cgcgccccgg tgcgggtcct ggaaagccgt
     4921 tgcgacgact ctccgcaagg ccagcgggcg cgcggataaa agggaatatg ataccttatc
     4981 ccgttttcgc gaataaggct tctggcgccc cacgcgggca tttcctgtcg ggatcgccac
     5041 tcgcttaccg gtctgcctcc atgtccctag aacttgatca ggtcaagcgc atcgccgcgc
     5101 ttgcgcgaat cgccattggc gacgacgagg cgcaagcggt ccgcgaccag ttgaaccgca
     5161 tcctcgccat ggtcgaaacc atgcaggccg tcgataccac cggcatcgag ccgatggccc
     5221 atgcgcagga tctctcacag cgactgcgcg aggaccgggt cagcgaatcc gaccggcgtt
     5281 ccgacttcca gcggcttgcg cccgaagctg aagccggcct ctacctcgtt cccaaagtca
     5341 tcgaatgagc gggtcggcag acactgcctg agcccgcccc gtttcgaccc cattgatcgc
     5401 ttccatgact gacgccagcc tctccgaact gcgagcacaa ctcgacgcgc gcaaagtctc
     5461 cagccgcgaa ctgactcagg cctgcctcga gcggatcgcc gcgcgcaatg ccgaactcaa
     5521 tgcctttatc agcaccgacg ccgagaaaag cctgacactg gctgacgccg ccgatcgccg
     5581 gatcgcggcc ggcgaggcag ggccgctgac cggcattccg ctggcgcaca aggacatttt
     5641 ctgcgccaag ggatggcgga ccagttgcgg ctcgaagatg cttgccgact ttgtcagccc
     5701 ttacgattcg accgtcgtcg cccgctgcga cgccgccggc gcggtcaatc tcggcaagct
     5761 caacatggac gagttcgcga tgggctcgtc gaacgaaaat tcgcacttcg gcccggcccg
     5821 caatccttgg gacctgaacc gcgtgcccgg cggttcgagc ggcggttcgg ccgtggcggt
     5881 cgctgccggc ctggtttcgg ccgcaaccgg caccgatacc ggcgggtcga tccgccagcc
     5941 ggccgcttgg tgcggcctga ccggcctcaa gcccacctac ggcgcggtgt cgcgctgggg
     6001 tatggtcgcc tacgcttcgt cgctcgacca aggcgggccg atcgcccgct cggcggccga
     6061 ctgtgccgtg ctgctcaacg ccatcgccgg tttcgacgag aaggattcaa cctcggtcga
     6121 gcgccccgcc gaggattacg cgcgggcgct cgcggcgccc gccgaacggc ggctcaaagg
     6181 tttgcggatc ggtttgccga ctgccttttt cggcccaggc atcgcgcccg gcgtacgcgc
     6241 cgccgtagat gcggcgattg cgcagttccg tgaactcggc gccgagatcc gcgaagtgga
     6301 tctgccaaac gccagcctgt cgctaccggc gtactacgtc atcgcgctcg ccgaagccag
     6361 ttccaacctg tcgcgcttcg atggagtgcg ctacggctac cgggcgtccg aatacggcga
     6421 tctcgccgat atgtacgcca agtcgcgggc acaggggttc ggtgccgagg tgaagcggcg
     6481 catcctcgtc ggcacctacg tgctttcgca cggttactac gatgcctatt acctgcaggc
     6541 gcagaagatc cgccgcctga ttgccgacga tttccgtcga acatttgccg agtgcgacct
     6601 gatcgccggc ccgaccacgc cgacgacggc attccgaatc ggcgagaagt cggacgatcc
     6661 ggtgcagatg tacctcgacg acatctttac cgtaccggtc aacctcgccg gcctgccggc
     6721 gatgtcgatt ccctgcggca tggccgatgg cttgccggtc ggcctgcagc tgatcggtga
     6781 ttacttctcc gaggctcggc tgataggtgc tggcgatgca taccagcagg caacagactg
     6841 gcaccagcgg cgtccgggcg gggaggcacg caaatgagtc tcggcaactg ggaagtcgtc
     6901 ataggcatcg agacgcacgc gcagcttcag acccatacga agatcttttc gggcgcctcg
     6961 accgatttcg gcgccgagcc caacgtgcag gcctgtctag ttgacctcgc ctatcccggc
     7021 gtgctgcccg tgctcaaccg gcaggcggtc gaatgcgcga tccgcttcgg tctcgcggtc
     7081 ggcgcgcagg tggcgcctcg ttcggtcttc gcgcgcaaga actacttcta tccagatctc
     7141 cccaagggct accagatcag ccagttcgag ctgccggtgg tgcagggcgg cgccctgacg
     7201 atcctcagcg gcgacagtga aaagacgatc cggctgacgc gggcgcatct cgaggaggac
     7261 gccgggaaaa gccttcacga ggacttccat ggcaagaccg gtatcgatct caatcgcgcc
     7321 gggacgccgc tgctcgagat cgtttccgaa cctgatctcc gctcgtcagc cgaggccgtc
     7381 gcctacgcca ggacactaca cgcgctggtg cgttggatcg gcatctgcga cggcaacatg
     7441 caggaaggca gtttccgctg tgacgccaac gtctcggtac gtcgcccggg ggcgccgcta
     7501 gggacgcggc gcgagatcaa gaaccttaat tccttccgtt ttatgcagca ggcgatcgac
     7561 tacgaggtcc gttggcaaat cgagacgatc gaggacggcg gccgtatcga gcaggcaact
     7621 gtgcttttcg accccgatag tggagagacg cgggcgatgc gcagcaagga agacgcgcac
     7681 gactaccgat attttcccga ccccgacctg ctgccgttgc tgatcgccga cgactgggtg
     7741 tcgcgggtgc gcgcgacgct gcccgaactg cccaacgcca agcgcgctcg cttcgtcgcc
     7801 gattacggcc tgtcgaccta cgatgcgacg tcgctgactg cgtcgctcga caccgccgac
     7861 tactacgagg cggtcgtcgc gatcgccggg aaggcgcagg cgaagcctgc ggccaactgg
     7921 gtgatggtcg acctcgccgc caggttgaac aaggagggac tggagatcgc cgcctcgccg
     7981 gtttccgctg cccagctcgg cgggctggtg gcgcgcatcg gcgacgggac gatttccaac
     8041 agtatcgcca aaaaggtgtt cgaagcgctg tgggccggcg agggcgccgg cgccgacgag
     8101 gtgatcgaaa agcaggggtt gaagcagatc actgacaccg gcgcgatcga ggcgatggtc
     8161 gatgccgtga tcgccgccaa tccgcagaac gtcgccgaat tcagggcagg caaggaaaag
     8221 gccttcaacg cgctggttgg tcaggtgatg aaggcggcca agggcaaggc caatccgcag
     8281 caggtgaacg agctgttgcg gcgcaagctc gccgagtgat caggcgtcgg taggcggcgc
     8341 cgtctggccg ccgtcggcga ctcccgacta gcgttcgagc cgctcgctgc gaccgcgcac
     8401 ggtcaggttg agatagcgct cgcgctcatc ggcgaagcgc gcggccattc cctcgaattc
     8461 cttgcgcttg acggcgatca ggtcggtctg cgacctgatt tcgatctccg tgtcgcgcag
     8521 gccgcgcttg atttcgggcg ggatcgcctt cttcttgtag aactctgcct cgttctggta
     8581 cttggcgcgg cgcttgttcg ccacgtcgat ccggcgttcg gcctcttcga ttgccttggt
     8641 gacgtcctcg cgggcgcgct cgtgagcaat gtcgatgtca cggacgttgc cgtaggtgga
     8701 cagcagaacg cggtctaggc gcagctgttc ggcggcggcc tcctcggcct cgcgtcggcg
     8761 ccgctcctct gcctcgcgtg ccttgcgttc gctcgggctg atgaagccct cgaccttggt
     8821 cgtatagccg cccggtccgc gaaccgtgtg cggcctgccg acgcattgcg tcggcaggcg
     8881 gtcgccgcag accttgcgcc cgctgttgtc cttgcagcag tacaggtttt gcgcgatcgc
     8941 cggcgctgca ctcagcgcga gcagcatcgt catcagggca gcagttttat gcggcaacgc
     9001 cataggtgct ccggtattgg gcgacggcgc tgcgatgctg cgcgagatcg ccgcgggcgt
     9061 cgagaaagct catcaagtcg gtcagcgagg cgacgctgat caccgggatt cgatagtcgc
     9121 gctcgacttc ctgtaccgcc gacaactcgc cctgcccgcg ctccattcgg tcgagggcga
     9181 tgaccacgcc ggccggcgtg gcgccctgcg cgcggatcag ttcgaccgac tcgcgcaccg
     9241 aggtgccggc cgagatcacg tcatcgatga tcaacacgcg gcccttgagc ggcgcgccga
     9301 tcagcatgcc accctcgccg tggtccttgg cttcctttcg gttgaaggcg aagggcaggt
     9361 tgcggccctc gcgcgcgagc gccatcgcgg taccggcggc gagcgcgatg cccttgtacg
     9421 cagggccgaa cagcatgtcg aaggcgacgc cgctggcgag aatcgtgcgc gcgtagaatt
     9481 gcgccagccg gtcgagcgag gcgccgtcgt tgaagaggcc ggcattgaag aagtagggcg
     9541 agagacggcc cgccttggtc ttgtattcgc cgaaacgcag cacctgctgc gccaccgaaa
     9601 aggcgatgaa atcatccgct acgcgcatcg ggttcgcggc cttcgtgccg ggagcttggg
     9661 gagaactcat gttacgcatc atcacgctca acctcaacgg catccgcgcc gcctggcgca
     9721 aaggatttca cgactggctg gcggcgcagg gcgcttcagt ggtatgcctg caggaactga
     9781 aggcaaggga tgccgatctg tcggccgaga tgcgcgccgc ggctgggctc tccggccact
     9841 tcttgcacgc tgagcaggcc ggttacagcg gcgtcggcct ctatctcgac cgccccccgg
     9901 cagcactggc gaccggtatc gatgacgccg agttcgacgc cgaggggcgc gtgcttcgcg
     9961 ccgatttcga cgatttctcg atcgtttcgc tctacctgcc ctcgggttca tcgagcgagg
    10021 agcggcaggc ggcgaagttt cgtttcatgg cgcgcttcgt cacggtcatg gacgagtggc
    10081 ggcgaagcgg gcgcgaattc atcatttgcg gcgattggaa cattgcgcat cgcgagatcg
    10141 acctgaagaa ctggaaatcg aaccagaaga actcgggttt cctccccgag gagcgcgcct
    10201 ggttgtccgg cgtattcgag gagcagggct gggtcgacgt ctatcgtcag ctgcatcccg
    10261 aggcgaccga tgcctgctac acatggtggt cgaaccgcgg acaggcttgg gccaagaatg
    10321 tcggctggcg gctcgattac cagatcgcca cgccgggcat cgcggcgtgc gccaggacag
    10381 cggagatcta caaggcgcag cgcttctccg accacgcgcc gctgatcatc gactacgact
    10441 ggccggcctg agctgctgcg cccgacagta atgcgagcag tcgggcgtat tcgtccggat
    10501 ccttgatcag taccatcgtg ccgctgcgcg gcgcctgcgc gtcgagcagg cgcgagagcc
    10561 agaagcgcag cgcagcaacc tgccgcatcg ccggccaagc gcgccgttcg gcggccgtca
    10621 gcgggcgcct tcgtccgtag gcagcgagca gcgcgtcttc gcgtgctgcg tcaggcagac
    10681 cagcgaccac gcaccagtcg ttggcgacga ccgccaggtc gaagagcagc gcgtcttcgc
    10741 cggcaaagta gaagtcgatg acgccgccca ggaccgcgtg gtcggcgtcc gcaaacagcg
    10801 cgttgtcgcg gaagaggtcg gcgtggacca ggccgcgcgg aagatcgagc gcggccagtt
    10861 gcgcatcggc tgccagcgct tcgtcgagca ggcggtgctg ttcagccggc agccattggc
    10921 gaacctccgc tgctgccgtt gaccgccaga caacgccgcg cggatttggc cagcgggcga
    10981 cgaagtcgga ttgcgctgcg tgcatcgccg ccagccagtc gccgatcgcc gtgcagtggg
    11041 ccgccgccgg ctgctcgaca tcgcgccccg ggatgcggct gaccagtg
//
LOCUS       DATDAA010000276         8280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_159962,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000276 DATDAA010000000
VERSION     DATDAA010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159962"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /gene="nuoL"
                     /locus_tag="VIS73_08715"
     CDS             complement(<1..1459)
                     /gene="nuoL"
                     /locus_tag="VIS73_08715"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY5763277.1"
                     /translation="MDMKSLYLLVPLAPLVGAAIAGLFCRVIPRWVAHTVTIAGVAIA
                     MIASMVIFKDVMAGNTFNGNVYTWMTSGDFGFHVGFLIDSLTVTMMLVVTSVSLMVHI
                     YTIGYMSEDPGYNRFFSYISLFTFSMLMLVMSNNFMQLFFGWEAVGLVSYLLIGFWYT
                     RPTAIYANLKAFLVNRVGDFGFILGIGLVLAYFGSLDYATVFAKAPDFASTTINLWGA
                     AEWSLMTVVCILLFIGAMGKSAQVPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVA
                     RMSPLFELSTTALGFVIVIGAITALFMGFLGIIQNDIKRVVAYSTLSQLGYMTVALGA
                     SAYAAGIFHLATHAFFKALLFLAAGSVIIALHHEQDMRKMGGLKKYMPITYWTSVIGT
                     LALIGFPGFSGFFSKDALIEAVHLSGTWGAGYAFWCVLLGVFVTSLYSFRLLFLVFHG
                     EERIDPHAKEHLHESPWVVTGPLIALAIPSVFIGWL"
     gene            complement(1464..1769)
                     /gene="nuoK"
                     /locus_tag="VIS73_08720"
     CDS             complement(1464..1769)
                     /gene="nuoK"
                     /locus_tag="VIS73_08720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY5763278.1"
                     /translation="MLSLSHYLILGAILFAISVVGIFLNRKNLIVLLMAIELMLLAVN
                     MNFVAFSHFLGDVSGQIFVFFILTVAAAESAIGLAILVVLFRSLRTIHVDDLDSLKG"
     gene            complement(1824..2426)
                     /locus_tag="VIS73_08725"
     CDS             complement(1824..2426)
                     /locus_tag="VIS73_08725"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY5763279.1"
                     /translation="MDFKTGVFFFLATILVFAAVRVVTARNPVVAALFLVLSFFSAAG
                     IWLLLTAEFLAMTLIVVYVGAVMVLFLFVVMMLDINIERMRKGFWSYLPLSALVGGLM
                     VAEMWMVLKAQYSGVAQSAVPAVAADYSNTKELGRLIYTDYVYPFELAAVILLVAIVA
                     AIALTHRGPKKTRYTDPAKQLAVKAKDRMRVLQMPAEPKE"
     gene            complement(2436..2924)
                     /gene="nuoI"
                     /locus_tag="VIS73_08730"
     CDS             complement(2436..2924)
                     /gene="nuoI"
                     /locus_tag="VIS73_08730"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY5763280.1"
                     /translation="MGAMKDVFKSLFLKELFQGMAVTGRYFFARKITVQYPEEKTPQS
                     HRFRGLHALRRYPNGEERCIACKLCEAVCPALAITIESEQRDDGSRRTTRYDIDLTKC
                     IFCGFCEEACPVDAIVESRVFEYHGEKRGDLYYTKPMLLANGDRYEQELAADRETDAP
                     YR"
     gene            complement(2935..3984)
                     /gene="nuoH"
                     /locus_tag="VIS73_08735"
     CDS             complement(2935..3984)
                     /gene="nuoH"
                     /locus_tag="VIS73_08735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY5763281.1"
                     /translation="METLMEFGVGVFGGAWPVVWTLAKIVLIIAPLLLGVAYLTYFER
                     KVIAYMHQRIGPNRVGPLGLIQPIADGLKLLLKEIIVPANASSKLFLIAPMLCLGPAL
                     AAWAVVPFADGLVLADINAGLLYIFGMTSLGVYGVIVAGWASNSKYAFLGAMRCAAQV
                     VSYELAMGFALVCVLLVSSSLNLSDIVNGQGKGQFAEMGLGFLSWNWLPLLPMFVVYF
                     ISGVAETNRAPFDVAEGESEIVAGFHTEYSGMAFALFFLGEYANMILVAALTSILFLG
                     GWLSPIGFLPDGIWWLLIKMSAMLFLFLWFRATFPRYRYDQIMRLGWKVFIPLTLIWL
                     VVIAAWVVSPWNIWA"
     gene            complement(3984..6326)
                     /gene="nuoG"
                     /locus_tag="VIS73_08740"
     CDS             complement(3984..6326)
                     /gene="nuoG"
                     /locus_tag="VIS73_08740"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEY5763282.1"
                     /translation="MLEIEIDGKAAQVADGSTVIEAAQQLGVYIPHFCYHKKLSIAAN
                     CRMCLVQVEKAPKPLPACATPVTNGMKVFTHSELAVKAQKGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGDVKSRFKEEKRVVFHKNVGPLVSMQEMTRCIHCTRCVRFGQEI
                     AGIMELGMANRSNHAEIMTFVGRSVDSELSGNMIDLCPVGALTSKPFRYAARSWELAR
                     RKSVAPHDSLGSNLVVQVKGNRVMRVLPAENEAINECWLSDKDRFSYEGLNADDRLTR
                     PMVKQDGRWHEVDWNVALDYAAHGLKDVARTQGADAIAALAHPCSTLEELHLVQKLVR
                     GLGSGHVDSRPRAVDFTADGKTKGAPWLGMPIAALSELDSVLVVGSFLRKDHPLVAQR
                     LRLAAKKGTQVCLLGVAGDDPLMALAVNAVTAPSRLPKMLAGIAKAAAELKGATVAKE
                     LAEVVPCDNAKKIAAVLADGGEKKAILLGNVVTQHAQAAQLQALAQEVGQITGATVGF
                     LGEAANSVGAHVAKALPNEGALNARSMFESPRSAYLLFGIEPEFDCANPQLAVSVLKQ
                     AKLVVMATAFRNERALEYADVLLPIGAFVETSGTYINCEGRVQSVHGAVKPVGDARPG
                     WKVLRVLGNLTGVAGFDYESSEAVRGEMVSESTEFVGDRLDNAIAGVSLALPAAFEGL
                     QRIADVPIYRTDALVRHASSLQLARDSAAPTLRASAETLGKLGLKAGEQAQVKQGAGS
                     ATLTVALDDGVPSGCVRVAAACEETAALGEMFGAITVERA"
     gene            complement(6357..7685)
                     /gene="nuoF"
                     /locus_tag="VIS73_08745"
     CDS             complement(6357..7685)
                     /gene="nuoF"
                     /locus_tag="VIS73_08745"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY5763283.1"
                     /translation="MAILGAINPVLTKGWNGDAGWRLEHYVKRGGYDALKKVLSNQIP
                     QADVINEVKKSVLRGRGGAGFPTGLKWSFMPRSFPGDKYVVCNTDEGEPGTFKDRDLI
                     RFDPHSVIEGMAIAAYAMGASRGYNYIHGEIWELYERFEEALDEARAAGMIGKNILGS
                     GFDFELFAHHGYGAYICGEETALLESIEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FASVPYILREGGESFLNLGKENNGGTKLFSIAGHVNKPGNYEIPLGTPFSTLLEMAGG
                     MRGGRKLKAVIPGGSSSPVVPGATMMDTTMDYDSIAKAGSMLGSGAVIVMDETTCMVN
                     ILERLSYFYHEESCGQCTPCREGTGWLYQVIRRIENGEGRPEDLDLLNSVSDNIMGRT
                     ICALGDAAAMPVKSFVKHFRDEFAYHIEHKTCLVPKDVQWAGSGFFVAAE"
     gene            complement(7686..8159)
                     /gene="nuoE"
                     /locus_tag="VIS73_08750"
     CDS             complement(7686..8159)
                     /gene="nuoE"
                     /locus_tag="VIS73_08750"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEY5763284.1"
                     /translation="MLTEQSLKRIDREVAKYPDGQAQSAVMAALAIAQEEKGWLEKDM
                     IEFVANYLKIPAMAAYEVASFYHMYHLEPVGKSVLTVCTCVPCALSGGRHAGEYLKEK
                     LGIGYNETTSDGRFTLKEGECFGTCGDAPLVMVNNRRMCSKMTPEQIDKLIVELP"
     gene            complement(8159..>8280)
                     /locus_tag="VIS73_08755"
     CDS             complement(8159..>8280)
                     /locus_tag="VIS73_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763285.1"
                     /translation="RAPGFIHLAAMDEMARGHMIADVVTIIGSQDIVFGEIDR"
BASE COUNT         1680 a         2586 c         2570 g         1444 t
ORIGIN      
        1 tgagccagcc gatgaacacg gacggtatcg cgagggcgat cagcggtccg gtgacgaccc
       61 acggcgactc gtgcaggtgc tctttggcat gcggatcgat gcgctcctcg ccgtggaaca
      121 ccaggaacag caaccggaag ctgtaaagcg atgtcacgaa tacgcccagc aatacgcacc
      181 agaaggcata acccgccccc caggtcccgg acaggtgcac ggcctcgatc agggcgtcct
      241 tggagaagaa gccagagaag cctggaaagc cgatcagcgc aagcgtcccg atcaccgacg
      301 tccagtaggt gatcggcatg tacttcttga gcccgcccat cttgcgcatg tcctgctcat
      361 gatgcagcgc gatgatcacg gagcccgccg cgaggaacag cagcgccttg aagaaagcgt
      421 gggtcgcgag atggaagatg ccggcggcgt acgcagacgc gccgagcgcc accgtcatgt
      481 agccgagctg cgagagcgtc gagtaggcga cgacgcgctt gatgtcgttc tggatgatgc
      541 cgaggaagcc catgaacagg gccgtgatcg cgccgatgac gataacgaac ccgagcgccg
      601 tggtcgacag ctcgaacagc ggcgacatcc gagcgaccat gaaaatgccg gccgtaacca
      661 tcgtcgcggc atggatcagc gccgagatcg gggtcgggcc ctccatggaa tcgggcagcc
      721 agacgtgtag cggcacctgc gccgacttgc ccatcgcgcc gatgaacagg aggatgcaga
      781 cgacggtcat cagcgaccat tcggcggcac cccacaggtt gatcgtggta ctagcgaagt
      841 cgggcgcctt ggcaaagacg gtcgcgtaat caagcgagcc gaagtaggca aggacgagac
      901 cgatgccgag gatgaaaccg aagtcgccaa cgcgattgac caggaaggcc ttgaggttgg
      961 cgtagatggc cgtcggccgc gtgtaccaga agccgatcag caggtaggaa accaggccta
     1021 cggcctccca gccaaagaag agctgcatga agttgttgct catgacgagc atcaacatcg
     1081 agaaggtgaa cagcgagata tagctgaaga agcggttgta gcccggatcc tcgctcatgt
     1141 agccaatggt gtagatgtgc accatcagcg acaccgaggt gaccaccagc atcatcgtca
     1201 cggtcaggct gtcgatcagg aacccgacgt ggaaaccgaa gtcgccgctg gtcatccagg
     1261 tatagacgtt gccgttgaag gtattgccgg ccatcacgtc cttgaagatc accatcgacg
     1321 cgatcatcgc gatcgccacg ccggcgatcg tcaccgtatg cgcgacccag cgcggtatga
     1381 cgcggcagaa caggccggcg atggcggcgc caaccagcgg cgccagcgga acgagcaggt
     1441 agaggctttt catgtccatg cgatcaaccc ttcaggctgt cgagatcatc gacgtggatc
     1501 gtccgcagcg agcggaagag cacgacgaga atggcaaggc cgatcgccga ctctgctgcg
     1561 gcaacggtca ggatgaagaa gacgaagatc tggcccgaaa catcgcccag gaagtgcgag
     1621 aaggccacga aattcatgtt gacggcgagg agcatcagct cgattgccat cagcagcacg
     1681 atcaggtttt tccggttgag gaaaatgccc acgacgctga tcgcgaacaa aatcgcaccg
     1741 agtatcaggt aatgcgacag cgaaagcaca ggaacctccc acgcaagggg tgcgaacccc
     1801 tcgccgttat gctcagttaa acgttattcc ttcggctcgg cgggcatctg cagaacccgc
     1861 atgcgatcct tggccttgac cgccagctgc ttcgcggggt ctgtgtaacg ggtcttcttg
     1921 ggcccgcggt gcgtcagtgc aatcgccgcg acgatggcga cgagcaggat caccgccgcc
     1981 agctcgaacg gatacacgta gtcggtatag atcagccgcc ccagttcctt cgtgttgctg
     2041 taatccgccg cgacggccgg caccgccgac tgggcaacgc cggaatactg tgccttgagc
     2101 accatccaca tctctgcaac catcaggccg ccgaccagcg ccgacagcgg caggtagctc
     2161 cagaaaccct tgcgcatgcg ctcgatgttg atgtcgagca tcatcacgac gaagaggaaa
     2221 agcaccatca ccgcgccgac gtagacgacg atcagcgtca tcgcgaggaa ttccgccgtc
     2281 agcagcaacc aaatgccggc cgccgagaaa aaggacagca cgagaaagag cgccgcgaca
     2341 accgggttgc gcgcggtgac gacgcgcacc gccgcgaaca ccaagatggt cgcgagaaag
     2401 aagaaaacgc ctgtcttgaa atccatgttt gcgtctcagc ggtaaggggc gtcggtttcg
     2461 cggtcggcag caagttcctg ctcgtagcgg tcgccgttag ccaacagcat gggcttggtg
     2521 tagtagaggt caccacgctt ttcgccgtgg tattcgaaga cccgcgactc gacgatggca
     2581 tctaccgggc aggcttcctc gcagaatccg cagaagatgc acttggtcag gtcaatgtcg
     2641 tagcgcgtgg tgcggcgtga gccgtcatcg cgctgctcag actcgatggt gattgccagc
     2701 gccgggcaca ctgcctcgca caacttgcag gcaatgcagc gttcttcgcc attgggataa
     2761 cggcgcagcg cgtgcagccc gcggaagcga tggctctgcg gtgtcttttc ttcggggtac
     2821 tggaccgtga tcttgcgggc gaagaagtag cgccccgtta cggccatccc ctgaaacagt
     2881 tccttgagga acaggctctt gaatacgtct ttcatggcac ccatgactgt ctcctcaggc
     2941 ccagatgttc cagggcgaaa ccacccaggc ggcgatcacg acgagccaga tcagcgtcag
     3001 cggaatgaag accttccagc ccagacgcat gatctggtcg taccggtagc gcgggaaggt
     3061 cgcgcggaac cagaggaaca ggaagagcat ggccgacatc ttgatcaaca gccaccagat
     3121 gccgtccggc aggaatccga tcggcgacaa ccagccgccg aggaagagga tcgaggtcag
     3181 cgccgcgacc aggatcatgt tggcgtattc gccgaggaag aacagcgcga aggccattcc
     3241 cgaatactcg gtgtggaagc cggcaacgat ctccgactcg ccttcggcaa cgtcgaaagg
     3301 cgcgcggttg gtctcggcga cacccgagat gaagtagacg acgaacatcg gcagcagcgg
     3361 cagccagttc cacgagagga agcccagacc catttcggca aactgcccct tgccctgccc
     3421 attcacgatg tcgctgagat tcaggctgct cgacaccagc agcacgcaga ccagcgcgaa
     3481 acccatcgcc agttcatacg agaccacctg cgcggcgcaa cgcatcgcgc cgaggaaagc
     3541 gtacttggag ttcgacgccc atccggcgac gatgacgccg tagacgccaa gcgaggtcat
     3601 gccgaagatg tagagaaggc ccgcgttgat gtcggcgagc accaggccgt cggcaaacgg
     3661 aaccaccgcc caggccgcca gcgcggggcc aagacagagc atcggcgcaa tcaggaagag
     3721 cttcgaactg gcattggccg ggacgatgat ctccttgagc aggagcttca ggccatcggc
     3781 gatcggctgg atcaggccca gcgggccgac ccggttgggg ccaatccgct ggtgcatgta
     3841 ggcaatgacc ttgcgttcga agtaggtcag gtaagcgacg ccgagcagca agggagcgat
     3901 aatcaggacg atcttcgcca gcgtccagac caccggccat gcgccgccga agacgccgac
     3961 accgaattcc atcagggttt ccatcacgca cgctccacgg taatcgcgcc gaacatctcg
     4021 ccgagcgctg ccgtttcttc gcaggcggcg gccacgcgta cgcagcccga aggcacgccg
     4081 tcgtcgagcg ccactgtcag ggtcgccgaa cccgcgccct gcttcacctg cgcctgttca
     4141 ccggccttca ggccgagctt gccgagggtt tcggcagatg cgcgcagcgt cggcgcagcc
     4201 gaatcgcgcg ccagctgcag cgacgaggca tggcgaacca atgcgtcggt ccggtagatc
     4261 ggtacgtcgg caatgcgttg cagaccctcg aaggccgccg gcagcgccag cgacacgccg
     4321 gcaatggcgt tgtcgaggcg gtcgccgaca aactcggtac tttcggagac catttcgccg
     4381 cgcaccgctt cgctcgactc gtagtcgaac ccggcaacgc cggtcaggtt gccgagcacg
     4441 cgcaggacct tccagcccgg acgcgcatcg ccgaccggct tgacggcgcc atgaacgctc
     4501 tgcacccggc cttcgcagtt gatgtaggtg cctgaggtct cgacgaaggc accgatcggc
     4561 agcagcacat cggcgtactc gagcgcgcgc tcgttgcgga aggcggtcgc catcacgaca
     4621 agtttcgcct gcttgagcac ggagacggcg agttgcggat tagcgcaatc gaattcgggt
     4681 tcgataccaa acagcaggta ggcactgcgc ggcgattcga acatcgaacg cgcgttgagc
     4741 gcgccctcgt tcggtaatgc cttcgcgaca tgcgcgccaa cactgttggc ggcctccccg
     4801 aggaaaccga ccgtcgcgcc ggtaatttgc ccgacttcct gcgccagcgc ctgcagctgt
     4861 gcagcctgcg cgtgctgggt gacgacattg ccgagcagga tggctttctt ctcgccaccg
     4921 tcggcaagca cggcggcgat cttcttagcg ttgtcgcagg gaacgacctc ggccagttcc
     4981 ttggcaacgg tagcaccctt gagctcggcg gccgcctttg cgatgccagc gagcatcttc
     5041 ggcaagcgcg acggtgcggt gactgcgttg acggccagcg ccatcagcgg atcgtcgcca
     5101 gcgacaccaa gcaggcagac ctgcgtcccc ttcttggcgg cgaggcgcag acgctgcgcg
     5161 accaacgggt gatccttgcg caggaagctg ccgaccacga gcaccgaatc gagttcggac
     5221 agcgcggcga tcggcatgcc aagccagggc gcgcccttgg tcttgccatc ggccgtgaaa
     5281 tcgacggcgc ggggacgcga atcgacatgt cccgaaccca gcccgcgaac tagcttctga
     5341 accaggtgga gctcttcgag cgtcgagcag ggatgcgcca gcgcggcaat cgcgtcggcg
     5401 ccctgcgtgc gtgcgacatc cttcaggcca tgggcggcgt aatcgagtgc gacgttccag
     5461 tcgacctcgt gccagcggcc atcctgtttg accatcgggc gggtcaagcg gtcgtcggcg
     5521 ttgagccctt cataggaaaa acgatccttg tcggacagcc agcattcatt gatcgcctcg
     5581 ttctcggccg gcagcacgcg catcacgcgg ttgcccttga cctgaacgac gaggttcgaa
     5641 cccagcgaat cgtgcggcgc caccgacttc cggcgagcca gttcccagct gcgcgcggcg
     5701 tagcggaagg gcttgctggt cagcgcgccg accgggcaaa ggtcgatcat gttgcccgac
     5761 agctcggaat cgacgctgcg accaacgaag gtcatgattt ctgcgtgatt gctgcggttg
     5821 gccatgccga gttccatgat gccggcgatc tcctggccaa agcggacgca gcgcgtgcaa
     5881 tggatgcagc gcgtcatctc ctgcatcgac accagcggcc cgacgttttt gtggaagacg
     5941 acacgcttct cttccttgaa gcggctcttg acgtcgccgt agccaaccgc cagatcctgc
     6001 aactggcact cgccgccctg gtcgcagatc gggcaatcga gcggatggtt gatcaggagg
     6061 aactccatca cgcccttctg cgctttgacg gccaactcgg agtgcgtaaa gaccttcatc
     6121 ccgttggtca cgggcgtcgc gcaggccggt agcggcttgg gcgccttctc gacctgcacc
     6181 aggcacatgc ggcagttcgc cgcgatcgag agtttcttgt ggtagcagaa atgcggaatg
     6241 tagacgccaa gctgctgggc ggcctcgatc accgtgctgc cgtccgctac ctgcgcggcc
     6301 tttccgtcga tttcgatttc caacatgtcg ttaatttccg atccgtgggc cgcgcgtcat
     6361 tcggcggcga cgaagaaacc gctgcccgcc cactgaacgt ccttgggcac caggcaagtc
     6421 ttgtgttcga tgtgataggc gaactcgtcg cggaaatgct tgacgaagct cttcaccggc
     6481 atcgccgcgg cgtcgccgag cgcgcagatg gtccgcccca tgatgttgtc gctgacgctg
     6541 ttgagcaggt cgagatcttc cggacggcct tcgccgttct cgatgcggcg gatgacctga
     6601 tacagccagc cggtaccctc gcggcagggc gtgcactggc cgcaggattc ctcgtgatag
     6661 aagtacgaga gccgctcgag gatattgacc atgcaggtgg tctcgtccat cacgatcacc
     6721 gcacccgatc caagcatcga cccggccttg gcgatcgagt cgtagtccat cgtcgtatcc
     6781 atcatcgtcg caccggggac gacgggcgac gaggagccac ccgggattac cgccttcagc
     6841 ttgcgtccgc cacgcatgcc gcccgccatc tcaagcagcg tcgagaacgg cgtaccgagc
     6901 gggatttcgt agttgccggg cttgttgacg tggcccgcaa tcgagaacag cttggttccg
     6961 ccgttgttct ccttgccgag attgaggaag gactcgccgc cctcgcgcag gatgtacggg
     7021 accgatgcga aggtctcggt gttgttgatt gtcgtcggct tgccgtaaag gccgaacgag
     7081 gccgggaaag gcggcttgaa gcgcggctgc cccttcttgc cctcgatcga ttcgagcagc
     7141 gccgtctctt caccgcagat gtaggcgccg tagccgtgat gggcgaacaa ctcgaagtca
     7201 aatcccgaac cgaggatgtt cttgccgatc atgccggccg cacgcgcctc gtcaagggct
     7261 tcttcgaagc gctcgtagag ctcccagatc tcgccgtgga tgtagttgta gccacggctc
     7321 gcacccattg cgtaggccgc gatggccatg ccttcgatga ccgaatgcgg gtcgaaccgg
     7381 atcaggtcgc gatccttgaa ggtgcccggc tcgccttcgt cggtgttgca gacaacgtac
     7441 ttgtcacctg ggaaggagcg cggcatgaag ctccacttga ggccggtcgg gaaacccgcg
     7501 ccgccacggc cccgcagcac cgacttcttc acctcgttga ttacgtcggc ctgcggaatc
     7561 tgattcgaca gaaccttctt cagcgcgtcg tagccaccgc gcttgacgta gtgttcgaga
     7621 cgccagccgg cgtcgccgtt ccagcctttg gtcaggactg gattgatcgc accgaggatc
     7681 gccatttacg gtagctccac gatgagtttg tcgatctgct cgggtgtcat cttcgagcac
     7741 atgcggcggt tgttcaccat caccagcggc gcatcgccgc aggtgccaaa gcactcgcct
     7801 tccttcagcg tgaagcgccc gtccgaggtc gtctcgttgt agccaatacc gagtttttcc
     7861 ttgaggtatt cgccggcgtg gcgcccgccc gacagggcac agggcacgca ggtgcatacg
     7921 gtcagcacgc tcttgccgac cggctcgagg tgatacatgt ggtagaaact cgccacctcg
     7981 taggccgcca tcgccgggat cttcaggtaa ttggcgacaa actcgatcat gtctttttcg
     8041 agccagccct tttcttcctg ggcaatggcc agcgccgcca tcacggccga ctgggcctgg
     8101 ccatcggggt acttggcgac ctcgcggtcg attctcttca gggattgttc ggtcagcatc
     8161 agcggtcgat ctcgccaaaa acgatgtctt gtgaaccaat gatcgtgacg acgtcggcga
     8221 tcatgtggcc gcgtgccatt tcatccatcg cagccaaatg gatgaagccc ggagcccgca
//
LOCUS       DATDAA010000277         2431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_404866,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000277 DATDAA010000000
VERSION     DATDAA010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404866"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..527)
                     /gene="accB"
                     /locus_tag="VIS73_08760"
     CDS             complement(60..527)
                     /gene="accB"
                     /locus_tag="VIS73_08760"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY5763286.1"
                     /translation="MDLRKLKKLIDLVQESGISELEVTEGEEKVRIAKHIPAPATHAP
                     MPTYYAQPAPAAASAPAATPAVAVAAPAGDDTPEGHIVKSPMVGTFYRSASPSVPAFV
                     EVGASVNKGDTLCIIEAMKLMNEIESDASGVVKAILIDNGQPVEFGEPLFVIG"
     gene            complement(580..1083)
                     /gene="aroQ"
                     /locus_tag="VIS73_08765"
     CDS             complement(580..1083)
                     /gene="aroQ"
                     /locus_tag="VIS73_08765"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY5763287.1"
                     /translation="MKKAKRQSGAAKAEAESPARARVLVLHGPNLNLLGIREPQHYGA
                     HTLAEINRALAVRADAAGVDLEAFQSNHEGALIERVHVAREQGVSFIVINPAGYTHTS
                     VALRDALAGVDIPFVEVHLSNVFSREPFRHHSYFSDLAVGVISGLGSEGYLLALEFAL
                     NRVVQPD"
     gene            complement(1215..1751)
                     /locus_tag="VIS73_08770"
     CDS             complement(1215..1751)
                     /locus_tag="VIS73_08770"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY5763288.1"
                     /translation="MNKPRTLIAVLIVVSAITGASLALYLKAGIPGAVSIPEASLAQL
                     QASSFPDVRNVDQPYSQWQGKIVVLNFWATWCPPCREEMPMFSRLQDELRDRDVQFVG
                     IGIDSPSAIKEFDLRTPMRYPLLIGGSQSLDLARALGNPSGGLPYTVVFDRAGKPVMS
                     RLGLIDEASLSAALQPLL"
     gene            complement(1785..2384)
                     /locus_tag="VIS73_08775"
     CDS             complement(1785..2384)
                     /locus_tag="VIS73_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763289.1"
                     /translation="MTRRRSNGTAHGNPHLQAQVAHAAARLMAEDGITDYGHAKRKAL
                     RTLGLSDSHPLPPNSEVEAALKSYLALYQADEQPLRLRELREEALRLMQFFAPYRPYL
                     IGSVLDGTAGRNSNIDIQLFADSAKDVEIFLLDHGIGYAHGQPRHDRAEAVFVIETDA
                     GGANLIVYPLAEERVVYRNREGRVRERARAQTVELLLAA"
BASE COUNT          409 a          748 c          832 g          442 t
ORIGIN      
        1 aggctggctg ccgcggctgt cgatctgtgg ggttttcggc acgagcctgt aaccggcgat
       61 cagccgatga cgaacagcgg ctcgccgaat tcgacgggtt gcccgttgtc gatgaggatg
      121 gccttgacca cgcccgacgc gtcggactcg atctcgttca tcagcttcat cgcctcgatg
      181 atgcacagcg tgtcgccctt gttgacgctg gcgccgacct cgacgaaggc cggtacagac
      241 ggcgaggccg agcggtagaa agtgccgacc atcggcgact tgacgatgtg gccctcgggt
      301 gtgtcgtcgc cggccggtgc cgcgacggcg accgcgggtg tagcggcggg ggcagacgct
      361 gcggccggcg ccggttgagc atagtaggtc ggcatcggtg cgtgggtggc cggcgctgga
      421 atgtgcttgg cgatacgcac cttctcctca ccctcggtga cttccagttc agaaatcccc
      481 gattcctgaa cgaggtcgat caacttcttc aatttacgaa gatccatgct ttctccttgc
      541 tgtgttcttg tactcgaagc gccggcgggc gctgtggcat cagtccggct ggacgacgcg
      601 attcagggcg aactcgagcg ccagcagata gccttcgctg ccgagtccgg aaatgacacc
      661 gaccgcaagg tcggaaaaat acgaatggtg acggaacggc tcgcgggaga agacgttcga
      721 cagatgtacc tcgacgaaag ggatgtcaac gccggccagc gcgtcgcgca gcgcgacgct
      781 ggtatgcgtg tagcccgccg gattgatgac gatgaagctg accccttgct cgcgggcaac
      841 gtgcacccgt tcgatcagcg cgccctcgtg attgctctgg aacgcctcga ggtcaacgcc
      901 ggccgcatcg gcacgaaccg cgagcgcgcg attgatctcg gccagcgtgt gggcaccgta
      961 atgctgcggt tcgcggatgc caagcaggtt gagatttggc ccgtgcagga ccagaactcg
     1021 ggcacgtgcc ggagattctg cttctgcctt agcggcgccg gattggcgtt ttgccttctt
     1081 catggccgca agtttgccgc agatcgccgc attttgtcca gtttcggctt tccgaaggcg
     1141 ctgccttggc ggcgcgcggc ctgtccgaag aatacccgat ccgcgcgctt gccgacggtt
     1201 cgcggccagc tggcctagag cagcggctga agtgccgcgc tgagcgaggc ctcgtcgatc
     1261 aggccgagcc gcgacatcac cggcttgccg gcacggtcaa agaccaccgt atacggcagg
     1321 ccgccgctgg gattgccgag cgcgcgggcc agatccaggc tctgcgagcc gccgatcagc
     1381 agcgggtaac gcatcggcgt gcgcaggtcg aattccttga ttgccgacgg cgaatcgatg
     1441 ccgatgccga cgaactgcac gtcccggtcg cgcaactcgt cctgcaggcg cgagaacatt
     1501 ggcatttcct cgcggcaggg ggggcaccag gtcgcccaga aattgaggac gacgatcttg
     1561 ccctgccact ggctgtaggg ttgatcgaca ttgcgcacgt cggggaagct gctcgcctgc
     1621 aactgcgcaa ggcttgcctc gggaatcgac accgcgccgg ggatgccggc cttgaggtag
     1681 agcgccagcg aggcgccggt gatcgccgag acgacgatca ggacggcgat cagcgttcgc
     1741 ggcttgttca acaacggcga tggcggcatc ggcaaagcaa gggttcaggc ggcaaggagc
     1801 agttcgaccg tctgtgcccg cgcccgttcg cgaacccgtc cctcgcggtt gcggtagacc
     1861 acacgttcct cggccagcgg atagacgatc aggttggcgc cgccggcatc ggtctcgatg
     1921 acgaagacgg cctcggcccg gtcgtgacgc ggctgcccgt gggcgtaacc gatgccgtgg
     1981 tcgagcagga aaatctcgac atccttggcg ctgtctgcaa acagctggat gtcgatgttc
     2041 gagttgcggc cggccgtgcc gtcgagcacc gagccgatca gataggggcg gtagggcgcg
     2101 aagaactgca tcaggcgcag cgcttcctcc cgcagttcac gcagccgcag cggctgttcg
     2161 tcggcctggt agagcgccag atagcttttc agcgccgcct cgacttcgct gttcggcggc
     2221 agcgggtggc tgtcggacag gccgagcgtg cgcaaggcct tgcgtttggc gtggccgtaa
     2281 tcggtgatgc catcctcggc catcaggcgg gcggccgcgt gggcgacctg cgcctgcaga
     2341 tggggattgc catgggcggt gccattcgag cgtcggcggg tcatctttcg ttcccgtaga
     2401 atcgcgggcc gcagcgattc taccggcgaa c
//
LOCUS       DATDAA010000278         2799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_264515,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000278 DATDAA010000000
VERSION     DATDAA010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264515"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VIS73_08780"
     CDS             <1..840
                     /locus_tag="VIS73_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HEY5763290.1"
                     /translation="YRNTVRAYWLTGNASAGGLAQRLAGSSELFRAGGRQPQSSVAFI
                     AAHDGFTLHDLVSYARRHNEANGEGNRDGQSDNHSTNCGVEGETDDPAVFAQRRRLQR
                     ALLATLFVSQGVPMLQAGDELGRTQRGNNNAYCQDNALAWIDWSSADVGLVDFVSRLA
                     DLRGRYPQLRRLDWLSGEADDDGNPDVLWWHPQGRAMHAADWNGSERAFAARLAGTMR
                     GEAALLLLFNPGDARDFVLPDGLWALQLSSADPDERAVVAGGTTRPVAANSLDILAER
                     GDQ"
     gene            837..2363
                     /gene="malQ"
                     /locus_tag="VIS73_08785"
     CDS             837..2363
                     /gene="malQ"
                     /locus_tag="VIS73_08785"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY5763291.1"
                     /translation="MSPPRLRRSGILLHPTSLPGAHGIGDLGAGARRFVDWLEAAGQK
                     AWQVLPLGTPGPGNSPYMSDSAFAGNALLIDLAALAEAGWLAPGRLAEAPGFPADAVN
                     YHGARRFKHACLAEAAARFYATAAADERAAFAQFRQAQAHWCEDHALFAVLTEQLGGP
                     WDRWPEPLARRQTAALARARREHAVAIDATVFAQWCFYRQWAALRAQAAAAGIAIIGD
                     MPIYVAQHSADVWANPGLFELDATGQARVIAGVPPDYFSETGQRWGNPLYRWPAHAAD
                     GYRWWAARLRHAFALFDSVRIDHFRGFESYWEIPAAAETAIDGRWRQGPGAGLFTALR
                     VQLGELPLIAEDLGIITPEVTALRENLGLPGMRILQFAFDGKPANPYLPHNYRADSVV
                     YTGTHDNDTTNGWWESLDEATRVQVREYLGSDGRAIHWDMIRASMASVADLAILPMQD
                     VLGLGSEARLNRPGEADGCWIWRLDESRIEHWHAPLLASLAERYGRGQIDGREKLPAR
                     "
     gene            complement(2360..>2799)
                     /locus_tag="VIS73_08790"
     CDS             complement(2360..>2799)
                     /locus_tag="VIS73_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY5763292.1"
                     /translation="LSAAIALAVFLPIVSDMSGCSGNQAVAVSMRELSLGIVKPYEVL
                     RVWIQEIKVGVLNGITLGILLGAVAWLWKDNYWLGLVVGAALALNTMVAVSLGGTVPL
                     VLKRIGVDPAVASGPILTTVTDMCGFFLVLSLATLVLPQLAGV"
BASE COUNT          455 a          949 c          961 g          434 t
ORIGIN      
        1 taccgcaata ccgtgcgcgc ctactggctg accggcaacg ccagcgccgg cggactagcg
       61 cagcgtctgg ccggatcgag cgagctgttt cgcgccggtg gtcgacagcc gcaaagcagc
      121 gttgccttca tcgccgctca cgacggtttt acgctgcacg atctggtcag ctacgccagg
      181 cgccacaacg aggccaacgg cgagggcaac cgcgatgggc agtccgacaa tcattcgacc
      241 aactgcggcg tcgagggcga gaccgacgac ccggccgtgt tcgcgcagcg ccgccgcctg
      301 cagcgggcac tgctggcgac gctgttcgtt tcgcagggcg tgcccatgct gcaggccggc
      361 gacgaactcg ggcgtacgca acgcggcaac aacaatgcct attgccagga caacgcgctg
      421 gcctggatcg actggtcgag cgccgatgtc gggctcgtcg atttcgtttc ccggctggcc
      481 gatctgcgcg gtcgctaccc gcagttgcgg cgactcgact ggcttagcgg cgaagccgac
      541 gacgacggca atcccgacgt gctgtggtgg catccgcagg gcagggcgat gcacgcagcc
      601 gactggaacg gcagcgagcg cgccttcgcc gcgcggctgg cgggaacgat gcgcggcgag
      661 gcagcgctcc tgctgctgtt caaccccggt gacgcacgcg atttcgtgct gcccgatggg
      721 ctttgggccc ttcagctgtc gagcgccgat cccgatgaaa gggctgtcgt ggccggtgga
      781 acgacgcgac cggtggcggc gaacagcctc gatatcttgg ccgagcgagg tgaccaatga
      841 gcccgccacg gctgcgccgc agcggcatcc tgctgcaccc gacttcgctg cccggcgccc
      901 acggcatcgg cgacctcggg gcaggtgccc gacgattcgt cgactggctg gaagcggccg
      961 gccagaaggc ctggcaggtg ttgccactcg gcacgccggg cccgggcaat tcgccctaca
     1021 tgagcgactc ggccttcgcc ggcaatgcgc tgctgatcga cctggccgcc ttggccgagg
     1081 ccggctggct cgcccccggg cgcctggcgg aagcgccggg tttcccagca gatgcggtta
     1141 actaccatgg cgcccgtcgc ttcaagcacg cctgcctggc ggaggcggca gcgcgtttct
     1201 acgcgacggc ggcggccgac gaacgggctg cgttcgcgca gttccggcag gcgcaggcgc
     1261 actggtgcga ggaccatgcc ttgttcgcgg tgctgaccga gcagctgggc ggcccctggg
     1321 accgctggcc cgagccgctg gcacggcgcc agacagcggc gctggctcgg gcgcggcgcg
     1381 agcacgcggt ggcgatcgat gcgaccgtct tcgcgcagtg gtgcttctac cggcagtggg
     1441 cggcgctgcg cgcgcaagcc gctgcggccg gcatcgccat catcggtgac atgccgatct
     1501 acgtcgccca gcacagcgcc gatgtctggg ccaaccccgg gctgttcgag cttgacgcca
     1561 ctgggcaggc gcgggtcatc gctggcgtgc cacccgacta cttcagcgaa accggtcagc
     1621 gctggggcaa tccgctctat cgctggccgg cgcatgccgc cgatggctac cgttggtggg
     1681 cggcgcgctt gcgccatgcc tttgcgctct tcgacagcgt tcgcatcgac cacttccgcg
     1741 gctttgagtc gtactgggag attccggccg cggccgaaac ggcgattgac gggcgctggc
     1801 ggcaggggcc gggcgctgga ctgtttacgg cgctgcgcgt acagctcggc gagttgccgc
     1861 tgatcgccga agacctcggc atcatcacgc ccgaggtcac cgccttgcgc gagaacctcg
     1921 gtttgcccgg catgcgcatc ctccagttcg cgttcgacgg caaaccggcg aatccgtacc
     1981 tgccgcacaa ctacagagcc gactcagttg tttataccgg cacccacgac aacgacacca
     2041 cgaacggctg gtgggaatca ctcgatgagg ccacgcgcgt gcaggttcgc gaatatctgg
     2101 gcagcgacgg cagggcgatc cactgggaca tgatccgcgc ctccatggct tcagtcgccg
     2161 acctggcgat cttgccaatg caagacgtgc ttggcctggg cagcgaggcg cgtctgaaca
     2221 ggccgggcga agccgacggc tgctggatct ggcggctcga cgaatcccgc atcgagcact
     2281 ggcatgcgcc gctgctcgcg tcgctggctg agcgctacgg gcgaggccag attgatgggc
     2341 gggaaaagtt gcctgcgcgc tagacgccgg cgagctgcgg cagcaccagt gtcgccaggc
     2401 tcagcacgag gaagaatccg cacatgtcgg tcaccgtggt caggatcggg cccgaggcga
     2461 ccgcagggtc gacgccgatg cgtttcagca ccagcggcac ggtcccgccc agagagaccg
     2521 ccaccatggt gttgagggcc agcgcggcac cgacgacgag ccccagccaa tagttgtcct
     2581 tccatagcca ggcgaccgcg ccgagcagaa ttccgagcgt gatgccgttc aagacgccga
     2641 ccttgatctc ctgaatccag acccgcagca cctcgtaggg tttgacgatg cccagcgaca
     2701 gctcgcgcat gctgacggca acggcctggt ttcccgaaca gccgctcatg tcggagacga
     2761 tgggcagaaa cacggccagc gcaatcgctg cgctcagcg
//
LOCUS       DATDAA010000279         2396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_20082,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000279 DATDAA010000000
VERSION     DATDAA010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20082"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VIS73_08795"
     CDS             <1..506
                     /locus_tag="VIS73_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY5763293.1"
                     /translation="AKKVPMVVLVNGGSASASEIVAGALQDHKRAVIMGTQTFGKGSV
                     QTVLPLPGNAAIKLTTARYFTPSGASIQAKGITPDIVAEEPALGDGSPHRIREADLAR
                     HLEGEDDAKKPEKSKTDKQAPGGKPADDEEDDTPRARFEFGGENDTQLKQALNLLKGL
                     QIMQARP"
     gene            523..957
                     /locus_tag="VIS73_08800"
     CDS             523..957
                     /locus_tag="VIS73_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763294.1"
                     /translation="MKRDPDHVKLAPQTAADLIKQREIGFAKFPPGQVAEAADELKAL
                     ERLEVTPAFETRQVQVGYSLTDYTLRELEEHLEDRGYHLDNTLMAKVMRALIHYVEDT
                     QLHNLGVPERLIKKSHEAYVRAWEQHAHGDRDETPPEWREYQ"
     gene            complement(994..2064)
                     /gene="ribD"
                     /locus_tag="VIS73_08805"
     CDS             complement(994..2064)
                     /gene="ribD"
                     /locus_tag="VIS73_08805"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY5763295.1"
                     /translation="MARALALAAQGLWTTSPNPRVGCVLVRDGEIIGEGWHRKAGEAH
                     AEIEALADAKKRAPGRIAGATAYVTLEPCAHTGRTGPCVEALVKAGVGRVVAAMEDPN
                     PLVAGRGIAALRAAGIAVDTGPGAAEAAELNVGFVSRMNRGRPWVRVKLAATIDGRTA
                     LAGGESQWITGPEAREDGHRFRARACAVLTGSGTIAIDDARLTVRLPGVERQPQRVVI
                     DGALRISPRSRVLACGETWIATASDDAAAAAPLQATGAELLHLPGADGRVDLVALLGE
                     LARRGINELHVEAGPRLAGALMARGLVDELLCYLAPGLIGDSGRGLISLPAPASLSDR
                     VALRWHDIRQVGSDLRVIARLD"
     gene            complement(2126..>2396)
                     /locus_tag="VIS73_08810"
     CDS             complement(2126..>2396)
                     /locus_tag="VIS73_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEY5763296.1"
                     /translation="MGLAWRKRPVTVEAVDAAILRIEDKLLVLGEREIPSEKVGELVM
                     RELKKLDKVAYIRFASVYRNFEDAAEFSDAVREVTRAPAGRTRRS"
BASE COUNT          419 a          855 c          784 g          338 t
ORIGIN      
        1 cggcgaagaa ggtaccgatg gtggtactgg tcaacggcgg ctcggcctcc gcctcggaga
       61 tcgtcgccgg cgcgctgcag gatcacaagc gcgccgtgat catgggtacg cagaccttcg
      121 gcaagggttc ggtgcagacc gtgctgccgc tgccgggcaa cgccgcgatc aagctgacca
      181 cggcgcgcta cttcacgccc tcgggcgcat cgatccaggc caagggaata acgcccgaca
      241 tcgtcgccga ggagccggcg ctgggcgatg gctcgccaca ccgcatccgc gaagccgatc
      301 tcgcccgcca cctcgaaggc gaggacgatg ccaagaagcc ggaaaaatcc aagaccgaca
      361 agcaggcgcc gggcggcaaa ccggccgacg atgaagaaga cgacacgccg cgggcgcgct
      421 tcgaattcgg cggcgagaac gacacgcagc tcaagcaggc gctcaacctg ctgaagggcc
      481 tgcagatcat gcaggcacgc ccctgagccg gcaccaagca cgatgaaacg cgatcccgat
      541 cacgtgaagc tggcgccgca gaccgcggcc gacctgatca agcagcgcga gatcggattc
      601 gccaagtttc cgccgggaca ggtcgccgag gccgccgacg aactgaaggc gctcgaacgc
      661 ctggaggtca caccggcctt cgaaacccgc caggtccagg tcggctacag ccttaccgac
      721 tacacgctgc gcgaactcga agagcacctc gaagaccgcg gctaccatct cgacaacacg
      781 ctgatggcca aggtcatgcg cgcgctgatc cactacgtcg aggataccca gctgcacaac
      841 ctcggggttc ccgagcggct gattaagaag tcgcacgagg cctacgtccg tgcctgggag
      901 cagcatgccc acggtgaccg cgacgaaacg ccaccggagt ggcgcgagta ccagtaggaa
      961 tttgatgcgg ccgggcgatt ggcccgccgc agttcagtct aagcgggcga tgacgcgcag
     1021 atcgctgccg acctgccgga tgtcgtgcca gcgcagcgca acgcgatcgg agagactcgc
     1081 cggcgccggc aatgaaatca gcccgcggcc gctgtcaccg atgagtcccg gcgccagata
     1141 gcacagtagt tcatcgacca gtccacgcgc catcagcgcg ccggccaggc gcggcccggc
     1201 ctcgacatgc agctcgttga tgcctcggcg ggccagttca ccgagcaggg cgaccaagtc
     1261 gacccgcccg tcggcgcccg gcagatgcag cagctcggcg cccgtggctt gcagcggcgc
     1321 ggccgccgcc gcatcgtcgc tggcggtggc gatccaggtt tcgccgcagg cgagcacccg
     1381 cgaccgcggc gaaattcgca gtgcgccgtc aatgacgacg cgttgcggct gccgctcgac
     1441 gcccggcagg cggaccgtaa gtcgcgcgtc gtcgatggcg atcgtgccgc tgccggtgag
     1501 caccgcacag gcgcgggcgc ggaaacggtg gccgtcctcg cgcgcttcgg gcccggtgat
     1561 ccactggctc tcgccgccgg cgagcgcagt ccgtccatct atcgtcgccg ccagctttac
     1621 ccggacccag gggcggccgc ggttcatgcg cgacacgaaa ccgacgttca actcggccgc
     1681 ttccgccgcg cccggccccg tatcaacagc aattccagcc gcgcgcaagg ccgcgatgcc
     1741 gcggccggcg accagtggat tggggtcttc catcgccgcg acgacgcgcc cgactcccgc
     1801 cttaaccagc gcctcgacgc aggggccggt tcgcccggtg tgcgcgcagg gctccagcgt
     1861 gacgtaggcc gtcgccccag cgatgcgccc gggtgcacgt ttcttcgcat cggccagcgc
     1921 ttcgatttcg gcgtgcgctt cgccggcctt gcggtgccag ccttcgccta tgatttcgcc
     1981 gtctctgacg aggacgcacc caacccgcgg attggggctg gtggtccaca ggccctgcgc
     2041 ggcgagtgcc agcgcccgcg ccatgttcct gtagtcgtcg gcgtcggggg ctcccccggc
     2101 agatgaagtc gcgtcggcca cccgatcagc tgcgccgcgt tcggcccgcc ggcgcgcgcg
     2161 tcacttcgcg aacggcgtcg gagaactcgg ccgcatcctc gaagttgcgg tagaccgaag
     2221 cgaagcggat gtaggcaacc ttgtcgagct tcttcagttc gcgcatgacc agctcgccga
     2281 ccttctccga gggtatttca cgttcaccga gcaccagcag cttgtcctcg atgcgcagga
     2341 tcgccgcatc gacggcctcg acggtaaccg gtcgcttgcg ccacgccagc cccatc
//
LOCUS       DATDAA010000280         2089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_475103,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000280 DATDAA010000000
VERSION     DATDAA010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475103"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /gene="dctP"
                     /locus_tag="VIS73_08815"
     CDS             <1..596
                     /gene="dctP"
                     /locus_tag="VIS73_08815"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY5763297.1"
                     /translation="QLFTKPPVKELGDLKGLKIAGAGTNLRYLDGLGATGVAGPLTGY
                     YNKVQTGVVDGAFLWPEAAVRFKLMEVAPHMLKADLGSVNTKVVPVNAGTWKKLPPEV
                     KKVLQDVAIAYRDHLAKLAAEKGNSAEAAFKKRGGTITTMSDEARAAWAKGLPNLAKE
                     WAAKLDSQGKPGSKVLAAYMNALRAAGAKPLRHWDRE"
     gene            676..1134
                     /locus_tag="VIS73_08820"
     CDS             676..1134
                     /locus_tag="VIS73_08820"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY5763298.1"
                     /translation="MIGALLVIGLLILINADSLGRTLFSAPILGVIEIVELSLVAIVF
                     LALGDAIRAGRLTRSEGLLTLLRDRAPRVSQALRIIFDLVAAAFFGLIVVGGVPRAIE
                     SYERGEFKGTAQVFTVPEWPVKLIIVIGAIAGIMAFLVAVWREFHAGRRR"
     gene            1171..>2089
                     /locus_tag="VIS73_08825"
     CDS             1171..>2089
                     /locus_tag="VIS73_08825"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY5763299.1"
                     /translation="MDPVGIGILSVVLVVALIYAGLYVPVALGVVSVLGVWLMRDRLD
                     LSINLLTLAVGDAVGDYNFATIPLFMVMGLVVSRIGIGRDVYDVANDSFRRVKGGLGI
                     ATVAANALFASVTGSSIASASVFTRVSLPEMRRLNYGRRFCVGVVAGSSVLGMLIPPS
                     VMMIIYAFVAEQSVGHMFLAGVIPGVLLAASFAIGIVMMAHFTPGFVGGRQPDTDTPS
                     LSLATKAKMLGPVVLLVVAVLGGIYGGVVTATEAGALGALIALIIGFLRRALDRRSLW
                     EIFVEAGYVSAALLLLIIAANMYSRMLGVA"
BASE COUNT          322 a          612 c          713 g          442 t
ORIGIN      
        1 atcaactctt taccaagccg ccggtcaagg agctgggcga tctgaaaggt ctgaagatcg
       61 ctggcgccgg caccaacctg cgctatctcg acggcctcgg cgcgaccggc gtcgccggcc
      121 cgctgaccgg ttactacaac aaggtccaga ccggcgtcgt cgacggggcg tttctctggc
      181 cggaagctgc ggtgcgcttc aagctcatgg aagtggcgcc tcatatgctt aaggccgacc
      241 tgggctcggt caataccaag gtcgtgccgg tcaacgctgg aacctggaaa aagttgcccc
      301 ctgaagtgaa aaaggtgctg caggatgtcg caatcgctta ccgcgatcac ctggcgaagc
      361 ttgcagctga aaaaggtaat tctgccgagg ccgctttcaa gaagcgcggc ggcacgatca
      421 cgaccatgtc cgacgaggcg cgcgcagcgt gggccaaagg cctgcccaac ctcgccaagg
      481 agtgggcggc gaagctggat agccagggca aaccaggcag caaagtgctt gctgcttaca
      541 tgaacgcgct ccgtgcagcc ggggcgaagc cactgcgcca ctgggatcgc gagtaaccgg
      601 cgacagcgcg tgggcgtgga tgcgggatac ggcgcgggga tccggtggtt ctaccgggcc
      661 ctcgccgtgc tcaacatgat cggcgccctg ctggttatcg gtctgctcat tctgatcaat
      721 gcggattcgc tggggcggac gctcttttcc gcgcccattc tcggggtcat cgagatcgtc
      781 gagctttcgc tggtggcgat cgtgtttctg gcgctgggtg atgcgatacg ggccgggcgc
      841 ctcacgcgct cggaggggtt gctgacgctg ctccgcgacc gcgcgccgag ggtctctcag
      901 gcgctgcgga taatttttga cctcgtcgcc gcggcgtttt tcgggctgat cgtcgtcggg
      961 ggcgttccga gggcgatcga gtcctacgag cgcggggaat tcaagggcac ggcgcaggtc
     1021 ttcacggtgc cggaatggcc ggtgaagctg atcatcgtca tcggcgcgat cgccggcatc
     1081 atggcgttcc ttgtggcggt gtggcgcgag ttccacgccg gccgccgccg atgagcgggt
     1141 gagcggtagc gttccctcac gaagcacgac atggatcctg tcggaatcgg catattgtcg
     1201 gtagtgctgg tcgttgcgct gatctacgcg gggctatacg tcccggtggc gctgggagta
     1261 gtttcggttc tgggcgtgtg gctgatgcgc gaccgcctcg atctttccat caatctcctt
     1321 acgctcgcgg tcggcgacgc ggtcggcgat tacaactttg cgaccatacc gcttttcatg
     1381 gtgatgggcc tggtcgtaag ccgcatcggc atcgggcgcg acgtctatga tgtcgccaat
     1441 gattccttcc gccgcgtgaa gggcggcctc ggaatcgcga ccgtggcggc gaatgcgctg
     1501 tttgcgtcag tcacgggctc ttcgatcgcg tccgcgtccg tgttcacacg ggtgtcgctg
     1561 ccggagatgc gcaggctcaa ctacgggcgc cgcttctgcg tcggtgtcgt tgctggaagt
     1621 tcggtgctcg gcatgctcat tccgccgagc gtgatgatga tcatttacgc tttcgtggcc
     1681 gagcagtcgg tcggtcatat gtttctcgcg ggggtgattc cgggcgtgct gctcgcggca
     1741 tcatttgcga tcggtatcgt gatgatggcg catttcacgc cgggctttgt cggaggccgc
     1801 cagcccgata ccgacacccc gagcctttcg ctggccacga aggcgaaaat gctggggccg
     1861 gtggtgctgc tcgtcgtagc ggttctgggc ggcatctacg gcggcgtggt cacggccacc
     1921 gaggctggtg cgctgggcgc actgattgcg ctcatcatcg gtttcttgcg tcgcgcgctg
     1981 gatcgcagga gcctgtggga aatcttcgtc gaggccggct acgtctctgc tgcactgctt
     2041 ttgttgatca tcgcggccaa tatgtacagc cgcatgctgg gggtcgccg
//
LOCUS       DATDAA010000281         2447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_509868,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000281 DATDAA010000000
VERSION     DATDAA010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509868"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VIS73_08830"
     CDS             <1..618
                     /locus_tag="VIS73_08830"
                     /inference="COORDINATES: protein motif:HMM:NF017773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5763300.1"
                     /translation="ALLQQRLGLDKRMLLFSWPSSSYLTYTGDARDLERSVDELNALL
                     TLAAEAVGHDRIVLMAHSMGSRGVVDALRARGQEAEFGSVIFIAPDMRRDVFSDNASM
                     LQQKASQVTVYTSDNDRVLWLSTTVNASGRLGVAKEFDLEHATVIDITPTGTNDISGH
                     LYHMLNPAVIEDLRALLETDPPDAERAFRRAPEDTPGFWRLEPVP"
     gene            673..2259
                     /locus_tag="VIS73_08835"
     CDS             673..2259
                     /locus_tag="VIS73_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5763301.1"
                     /translation="MKDKVAAFATVDPFEGMTAEDPGRAQNLVGGQWVSTTAVRDDIV
                     DPMNGRRFLQIPDTMDFGPFIDGLRSCPKTGLHNPMKNSERYVMLGRVCARAAALLAE
                     RDVSDYFTRLVQRVMPKSWNQCAGEVQITRVFLENFAGDGVRFLARGFSNPGDRTGQE
                     SRGYRWPYGPVVVVAPFNFPLEIPALQVMGALFMGNRPLVKIDSKVRIVFEQFLRLLI
                     HCGLPAGDIDMVHCRGENMGKLIGEARDIIRLVQFTGSSEVAERVAATMNGAVRIEDA
                     GFDWKIIGPDYDEQWLDYVAWQSDEDAYNASGQKCSAQSILFVHADWAPALLPKIGDL
                     AGRRTLDNLSIGPVLSWSNDRIKRHVDAVLALPGAELLFGGTPLAGHAIPECYGAWEP
                     TAVRVPLDAICGKHFELLTTELFGPFQVVATWSDDELDKVLDVFERMSQHLTAAVVSA
                     DPIFQQKVLGATVNGTTYCGMRARTTGAPQNHWFGPCGDPRGAGIGTPEAIRLTWSGH
                     REIILDQGALDSGWSTPPVA"
     gene            complement(2256..>2447)
                     /locus_tag="VIS73_08840"
     CDS             complement(2256..>2447)
                     /locus_tag="VIS73_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763302.1"
                     /translation="EVQWGVIASRDEFVIRPKRARYQVRAAQRDDYQALTRAMSEVLQ
                     QFSRDVAESLQGVMLEADD"
BASE COUNT          443 a          797 c          767 g          440 t
ORIGIN      
        1 gcattactgc aacagcgcct ggggctcgat aaaaggatgc tgctgttctc atggccctcg
       61 agctcttacc tgacctatac cggcgacgcc agggatctcg aacggagcgt cgacgaactc
      121 aatgcgctgc tgaccctggc cgcggaggcc gttggtcacg atcgcatcgt actgatggct
      181 cacagcatgg gatcgcgtgg cgtggtcgac gctctgcggg cacgtggcca agaagcggaa
      241 ttcggcagcg tgatcttcat cgcccccgat atgcggcgcg acgtgttcag cgataacgcg
      301 agcatgctgc agcagaaagc ctcccaggtc accgtctaca cgtcggacaa tgaccgtgtg
      361 ctgtggctgt cgacaaccgt caacgcatcg gggcgccttg gcgtggccaa ggaattcgac
      421 ctcgagcatg cgaccgtcat cgacatcacg ccgaccggca ccaacgatat ttccgggcat
      481 ctgtaccaca tgctcaaccc ggccgttatc gaagatcttc gcgcgctgct ggagacggac
      541 ccgccggatg cagagcgcgc atttcgtcgc gccccggaag acacgccagg cttctggcgc
      601 ctggagccag tcccctgaca ttctcctgcc agcattcgta caatgccggg tatgccaaga
      661 gagggactcg ccatgaagga caaagtagct gcattcgcca ccgtggatcc gttcgaggga
      721 atgaccgcgg aagatcccgg ccgggcacaa aacctcgttg gcggccagtg ggtatccacc
      781 acggcggtgc gcgacgatat cgtcgacccg atgaacggca gacgcttcct gcagatcccg
      841 gatacgatgg acttcgggcc attcatcgac ggcctgcgga gctgtcccaa gaccggcctc
      901 cacaacccga tgaagaactc cgagcgctac gtcatgctcg gccgcgtctg tgcacgtgcc
      961 gccgcgctgc tggccgagcg ggacgtttcc gactacttca cgcggctggt acagcgggtc
     1021 atgccgaaga gctggaacca gtgcgccggc gaggtccaga tcacccgggt cttcctcgag
     1081 aatttcgccg gcgacggcgt gcgcttcctg gcgcgggggt tttcgaaccc gggcgaccgc
     1141 accggccagg agtcgcgcgg ctaccgctgg ccttacgggc ccgtagtcgt cgtcgcaccg
     1201 ttcaacttcc cactggagat cccggcgctg caggtcatgg gtgctctgtt catgggcaat
     1261 cggccgctgg tgaagatcga ttcgaaggtc aggatcgtct tcgagcaatt cctgcgcctc
     1321 ctcatccatt gtggattgcc cgcgggcgac atcgacatgg tgcactgccg cggcgagaac
     1381 atgggcaagc tgattggcga ggcccgggac atcatccggt tggtgcagtt cacgggctct
     1441 agcgaggtcg ccgagcgcgt cgccgcgacc atgaacggcg ccgtgcgcat cgaggacgcc
     1501 ggcttcgact ggaagatcat cgggcccgac tacgacgagc aatggctcga ttatgtggcc
     1561 tggcagtcgg acgaggatgc ctacaacgcc tccggtcaga aatgctcggc gcagagcatc
     1621 ctcttcgtcc atgccgactg ggcacccgca ctgttgccga agatcggcga tcttgctggc
     1681 agacgtacgc tcgacaatct cagcatcggg ccggtgctgt cgtggagtaa cgatcgtatc
     1741 aagcgccatg tcgatgcggt gctggctttg ccgggcgccg aactgctgtt cggcggcaca
     1801 ccgctcgccg gccatgccat ccccgagtgc tacggcgcct gggagccgac cgcggtccgt
     1861 gtgccgctcg acgcgatctg cggcaagcac ttcgaactgt tgacgacaga gctgttcggg
     1921 ccattccagg ttgtcgccac ctggagcgac gacgaactgg acaaggttct cgacgtattc
     1981 gagcgcatgt cacaacacct cacggcggct gtcgtaagtg ccgacccgat cttccagcag
     2041 aaggtactcg gtgccaccgt gaatggcacg acctactgcg gcatgcgcgc gcggaccacc
     2101 ggcgcaccgc agaatcactg gttcggtccc tgtggcgacc cgaggggtgc gggcatcggc
     2161 acgccggaag cgatccgcct gacctggagc ggtcaccgcg agatcatact ggaccagggt
     2221 gcactggatt ccgggtggag tacgccgccg gtcgcttagt cgtccgcctc gagcatgacc
     2281 ccttgcagcg actccgccac gtcccggctg aactgttgca gcacctcgct catggcccgc
     2341 gtcaacgcct ggtaatcgtc gcgctgcgcc gcccggacct ggtagcgcgc ccgttttggc
     2401 cggataacga attcgtcccg ggacgcaatc acgccccact gtacctc
//
LOCUS       DATDAA010000282         3761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_230711,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000282 DATDAA010000000
VERSION     DATDAA010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230711"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VIS73_08845"
     CDS             <1..548
                     /locus_tag="VIS73_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY5763303.1"
                     /translation="YVATAKLLAQRIPGLQILVPLVTRETRLMFEAAMYRQGATELPI
                     RLLFGHAHDALGAADVALVASGTATLEAALLKCPHVIAYKMSPVSYQIMRRMAYQPYV
                     GLPNVLAGELLAPEILQDQATPEALADALLALYEDRERRSTIISRFTEIHRELRQGTA
                     ERAANAILEGLPAGAPALAVA"
     gene            535..1131
                     /gene="rnhB"
                     /locus_tag="VIS73_08850"
     CDS             535..1131
                     /gene="rnhB"
                     /locus_tag="VIS73_08850"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY5763304.1"
                     /translation="MQWPDATTCGVDEAGRGPLAGPVFAAAVLLDPDHPFPGIRDSKA
                     MSATARDLLAATIRERAIAWSVAEASVEEIDRLNILQATLLAMRRAVEILVPAARAAL
                     IDGNRLPLLAIPATTVVKGDSKVAAIAAASILAKTARDARMMSLHADHPEFGFDRHMG
                     YPTAAHFEAIERFGVTPHHRRSFAPVRRALAGESIRAS"
     gene            1128..1919
                     /locus_tag="VIS73_08855"
     CDS             1128..1919
                     /locus_tag="VIS73_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY5763305.1"
                     /translation="MNAARLSSADNPLIKRTRRLVSHARDRREAALVVLDGAHLLAAW
                     SAAGRDLEYVLLGDKGADDAEIVDLLASFPRATIHRVSDRLLASVSPVETPTGVVAVA
                     KQPFPAQAPDERADSVLLDGVQDPGNLGGILRTAAATGWRQVLLSPDCTQAWSPKALR
                     AGMGAQLALQIHEGVDLVAFLGGYQGLSLAADVGAQESLFALSHAPDLPLAWAFGAEG
                     RGLRPAVLAAVGKRVGIPMSAATESLNVGAAAALCLYEGWRRRSR"
     gene            2120..3052
                     /locus_tag="VIS73_08860"
     CDS             2120..3052
                     /locus_tag="VIS73_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5763306.1"
                     /translation="MDYRAPRWLPDGHAQTLWPVFAKEALPSYRRERWDTPDGDFIDV
                     DWIDGPQTVPCVVLFHGLEGSSRSHYARSLMLAVARQGWRGAVVHFRGCSGEANRLPR
                     AYHSGDADEIDWILRRFAGASSMPLFACGISLGGNALLKWLGERGTDAALVRATAAVC
                     APLDLAAANEVLTRGFSRVYTWHFLRTLIPTALERNRRFPGIIDPDRVLRARSLRDFD
                     DAVTAPLHGFRDADDYYTRSSAKAWLGGVQVPTLMINSANDPFLPITALPNAAQVASA
                     VRIEVPAAGGHVGFHAPDGPWLPCRLLRWYGDFL"
     gene            complement(3146..>3761)
                     /locus_tag="VIS73_08865"
     CDS             complement(3146..>3761)
                     /locus_tag="VIS73_08865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY5763307.1"
                     /translation="PRSYYEFLATVQAEGKHGEAFHYRTANTDALGWVLARVSGRNVA
                     QLLSERIWSRLGAEQDAYMSVDSIGTPFAGGGLNTGLRDLARFGEMVRNNGRYNGQQI
                     LPPDVVADIRRGANPDNFAKAGYRNLPGWSYRNMWWVTHNPHGAFMARGVHGQALYID
                     PMAEMVIARFASHPVAGNAANDPTTLPAFEALGRHLIQVTKPKS"
BASE COUNT          629 a         1246 c         1213 g          673 t
ORIGIN      
        1 cttacgtcgc gaccgcgaag ctgctcgccc agcgcattcc cggcctgcag attctggttc
       61 cgctggtcac acgcgagaca cgcttgatgt tcgaggccgc gatgtaccgg cagggcgcca
      121 ccgaactgcc gatccggctg ctttttggcc atgcgcacga tgcactgggt gcggcggatg
      181 tggcgctggt cgccagcggc acggcgacgc tcgaggcggc gttgttaaag tgcccgcacg
      241 tcatcgccta caagatgtcg ccggtttcgt accagatcat gcggcggatg gcctaccagc
      301 cctacgttgg cctacccaac gtgttggccg gtgaactgct ggcgcccgaa atcctgcagg
      361 accaggcgac gcccgaagcg ctggctgacg cgctgctcgc actctacgaa gaccgcgagc
      421 ggcggagcac gatcatttcc cgctttaccg agatccatcg cgagctgcgg cagggcacgg
      481 ccgagcgcgc cgccaacgcc atccttgaag gcctgccggc cggagccccc gcgcttgcag
      541 tggcctgatg ccacgacctg cggtgtcgat gaggcaggac gtgggccgct cgcgggcccg
      601 gtcttcgccg cggcggttct gcttgatccc gatcacccat ttccaggaat ccgggattcg
      661 aaagccatgt cggcaaccgc gcgtgatttg ctggcggcaa cgatccgcga gcgggcgatc
      721 gcctggtcgg tcgccgaagc atccgtcgag gagatcgacc ggctcaacat cctgcaggcg
      781 acgctgctgg cgatgcgccg cgccgtcgag atcctcgtcc cggcagcccg ggcggcactg
      841 atcgacggca accgcctgcc gttgctggcg atcccggcga ccaccgtggt caagggtgat
      901 tcgaaggtcg cggcgattgc cgccgcgtcc atcctcgcca agaccgcgcg cgacgcacga
      961 atgatgagcc tgcacgccga ccatcccgaa ttcggcttcg atcggcacat gggataccca
     1021 accgcagcgc atttcgaggc gatcgaacgt ttcggcgtca cgccacacca ccgccgcagc
     1081 ttcgctcccg tccggcgcgc gcttgccggc gagtcgattc gcgcatcgtg aacgcggctc
     1141 gactgagctc ggccgacaac ccgctgatca agcgcacgcg gcgcctcgtc agccatgcgc
     1201 gagaccggcg cgaggcggcc ctggtagtgc tcgacggcgc ccatctgctg gccgcatgga
     1261 gcgccgcagg acgggatttg gaatacgtgc tgcttggcga caagggtgcc gacgatgccg
     1321 agatcgttga cctactcgcg agctttccac gggccacaat ccatcgggtc agcgaccggt
     1381 tgctggccag cgtttcgccc gtcgaaacgc cgaccggcgt ggttgccgtc gcgaaacaac
     1441 catttcccgc ccaggctccc gacgaacgcg ccgactcggt cctgctcgat ggtgtgcagg
     1501 atcccggaaa cctcggcgga atcctgcgca cggccgcagc gaccggctgg cggcaggtat
     1561 tgctctcacc cgactgcacg caggcctgga gtccgaaagc cttgcgcgcc ggtatgggcg
     1621 cgcagctggc gctgcagatt cacgaagggg tcgatctggt agcctttcta ggcggctatc
     1681 agggcctgtc gctcgccgct gatgttggtg cacaggaatc gctgtttgcg ctatcccatg
     1741 cgcccgacct gccgctggcc tgggccttcg gcgccgaagg gcggggcttg cgacccgccg
     1801 tgctggctgc cgtcgggaaa cgcgtcggca tcccgatgtc cgctgcgacc gagtcgctta
     1861 atgttggtgc agccgcggcg ctctgtctct acgaaggctg gcggcggcga agccgctgat
     1921 ccagaccgct cgtttcatcg gctattggcc aatactgccg gagcacttgg cgaacgtgcg
     1981 cggcagcttg gggtcggatg gattcctgtg tcgaaccatt cggctaatcc gcaaagaggt
     2041 tagccggcga tggcagcccg tttccggctc gactcgcggt aaccatcgac tctgctgatc
     2101 tcgtttggaa aggggacgga tggactaccg tgcgccgcga tggctaccgg acggacacgc
     2161 gcaaacgctc tggccggtct tcgccaagga ggcgctgccg agttaccggc gcgagcgttg
     2221 ggatacgccg gacggcgact tcatcgacgt cgactggatc gacggcccgc agaccgtccc
     2281 ctgcgtcgtg ctgttccacg ggctcgaggg cagttcgcgc agccattacg cgcgcagcct
     2341 gatgctggca gtggcgcggc agggctggcg cggcgcggtc gtgcatttcc gagggtgttc
     2401 gggcgaagcc aaccggctgc cacgcgccta tcactcgggc gacgccgatg aaatcgactg
     2461 gatactgcgc cgctttgccg gcgcgagttc catgccgttg ttcgcctgcg gcatctcgct
     2521 ggggggaaac gccttattga agtggttggg cgagcgtgga accgacgcag cgctggtgcg
     2581 cgccaccgct gccgtttgcg cgccgctcga cctcgcagca gccaacgagg tgctgacccg
     2641 cggcttcagc cgagtgtaca catggcattt tctgcgaacg ctcataccga cggcgctgga
     2701 acgcaatcgc cggtttcccg gaattatcga tcctgaccgc gttcttcggg cgcgcagctt
     2761 gcgcgatttc gacgatgcgg tgacggcacc gctgcacggt ttccgcgacg ccgatgacta
     2821 ctacacgcgc agcagcgcca aggcctggct cggaggggta caggtgccaa ccctgatgat
     2881 caacagcgcc aacgatccgt tcctgccaat aaccgccttg ccgaacgccg cgcaagtcgc
     2941 ctcggcggtt cgaatcgagg tgcccgccgc aggcggccac gtcggattcc acgcgccaga
     3001 cggaccgtgg ctaccgtgcc gtcttctccg gtggtacggc gatttcctgt aagcatcagt
     3061 tcgagaacgc catcgtgcaa taagcctcgc attgcgcggt gagattgccg atccagcgcg
     3121 acatctgcgc ggtgcgtatg gcagttcagg acttcggttt cgtcacctga atcaaatggc
     3181 ggccgagggc ctcaaaggcc ggcaaagttg tagggtcatt cgcggcattg ccggctaccg
     3241 ggtgactggc gaaccgagcg atcaccatct cggccatcgg gtcgatgtac agcgcctgcc
     3301 cgtgtacgcc gcgcgccatg aaggcaccat gtggattgtg ggtaacccac cacatgttgc
     3361 ggtaactcca ccccggcagg ttgcgatagc cggctttagc aaagttatct gggtttgcgc
     3421 cacggcggat atcggcgacg acatccggcg gcaatatctg ctgcccgttg taccgaccgt
     3481 tgttacgcac catctcgcca aaacgcgcaa ggtcgcgcaa gccggtgttt aagccgcccc
     3541 cggcaaaggg cgtaccgatc gaatccactg acatgtaggc atcctgctct gcgcccaggc
     3601 gactccagat tcgctcagac aagagctgcg caacgttgcg gccgctaacc cgggccagaa
     3661 cccagccgag cgcatccgtg ttcgcagtac ggtaatggaa cgcttcgccg tgcttgcctt
     3721 ccgcctgcac ggtggccaaa aactcgtaat agcttctcgg t
//
LOCUS       DATDAA010000283         2832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_440275,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000283 DATDAA010000000
VERSION     DATDAA010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440275"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VIS73_08870"
     CDS             complement(<1..431)
                     /locus_tag="VIS73_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763308.1"
                     /translation="MSLLSPKRLCIGLAPDRVELARVHSGLRGAVVGEAQARVCVPEA
                     GAKPWTAALATLEQLLGEPTAKAGEARVTLSNHFVRYLVLPWDSALLGTDELHAAATQ
                     HFERVFGDTAVEWELRVSPGEFGEPVLACAIDRTLLEALDA"
     gene            complement(412..2391)
                     /locus_tag="VIS73_08875"
     CDS             complement(412..2391)
                     /locus_tag="VIS73_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY5763309.1"
                     /translation="MSSDQPDRPANAGAAAPPAKGRRRGRDGRGKRVSEAQADLLQDA
                     LPATETLAEAPPEVAAVPTAEPAAPVPAAATTAAAVAESSVVSAETRGEAPPQSGSGE
                     APLRVPREMIAEAMAESAASGIHVVDIVEARLGLSPPEFVACLGRTLGLDSLSMDELH
                     ALTPDFDVLPYAQAAQRECVALRDADGRLLAAIGEPFDPALRLWAETAIGEPFDWRLT
                     YSADLRALLSRQEESMRAMDSVLESGQSAAGVDADGTEALSIASISEGDSPAVKLVSS
                     TIYDALRANTSDIHIETGTNGLTIKYRLDGVLATLGNVQGRDLSDQVLSRIKVMAELD
                     ISERRVPQDGRFKVLIKGRAVDFRVSIMPSIHGEDAVLRILDKHTLIGEQQTLRLESL
                     GFDEAVKARIRALAKKPYGMLLVTGPTGSGKTTTLYAALNEINSGQDKIITIEDPVEY
                     QLNGVLQIPVNEKKGLTFSRGLRSILRHDPDTILIGEMRDAETAQIAVQAALTGHLVF
                     STVHANNVFDVISRSMAMGLEPYAFVSALNGIVAQRLMRKICAECAVDQEPDDELLTA
                     SGLKRKDVEGYTFKVGRGCGHCRGTGYQGRKAIAEILLVNDEIREMILRGEPVRRLRE
                     VAAAHGTHSLRESALECVRRGETTLQEINRVTFVA"
     gene            complement(2381..>2832)
                     /locus_tag="VIS73_08880"
     CDS             complement(2381..>2832)
                     /locus_tag="VIS73_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5763310.1"
                     /translation="LWQGPGIGERLRSYELARFYRTLAMLLNSGMPLPRSIDMSSALL
                     SPVLRDRLKAAAREIREGVPVSRAFERHGLTTPIARRLLGVGERSGRMAEMVDRIASF
                     YEDELTRWVDWFMLLFEPALMIIIGAIVGVILVLLYMPIFELAENVQ"
BASE COUNT          454 a          940 c          973 g          465 t
ORIGIN      
        1 caggcgtcga gcgcctccag cagcgtacga tcgatcgcgc aggccagcac cggctcaccg
       61 aactcgccgg gcgagacgcg cagctcccac tcgacggcgg tgtcaccgaa gacgcgctcg
      121 aagtgctggg tcgcggcagc gtgcagttcg tcggtgccga gcagagcgga atcccagggc
      181 agcacgaggt agcgaacgaa atggttggaa agcgtgacgc gtgcttcgcc ggctttcgcc
      241 gtcggctcgc cgagcagttg ctccaatgtc gccagcgccg cggtccaggg tttggcgcca
      301 gcctcgggaa cgcagacgcg cgcctgcgcc tcgccaacca cggcgccacg cagaccgctg
      361 tgaacccgcg ccagttcgac gcgatcgggc gcgaggccga tgcagagtcg cttaggcgac
      421 aaaagtgaca cggttgatct cctgaagcgt ggtttcgcca cggcgtacgc attcgagcgc
      481 cgactcgcgc aaggaatgcg taccgtgggc agcggcgacc tcgcgcaggc ggcggaccgg
      541 ttcgccgcgc aggatcatct cgcgaatctc gtcgttgacc agcaggatct cggcgatcgc
      601 cttgcgcccc tgataaccgg tgccgcggca gtggccgcag ccgcggccga ccttgaacgt
      661 gtatccctcg acgtcctttc gcttcaggcc cgaggcggtg agcaactcgt cgtcgggttc
      721 ctgatcgacc gcacactcgg cgcagatctt gcgcatcaga cgctgggcga cgatgccgtt
      781 aagtgccgaa acgaaggcat agggttcgag tcccatcgcc atcgagcggc tgatgacgtc
      841 gaagacgttg ttggcgtgca cggtgctgaa caccaggtgg ccggtgagcg cggcctgaac
      901 ggcaatctgc gcggtctcgg cgtcgcgcat ctcgccgatc agaatggtgt cggggtcatg
      961 ccgcaggatc gagcgcaggc cgcgcgagaa ggtcagcccc ttcttctcgt tcaccggaat
     1021 ctgcagcacg ccgttgagct ggtattccac cgggtcttcg atcgtgatga tcttgtcctg
     1081 gccgctgttg atctcgttca gggccgcgta cagcgtcgtg gtcttgccgc tgccggtcgg
     1141 cccggtcacc agcagcatgc cgtagggctt cttcgccagc gcccggatgc gcgccttcac
     1201 cgcttcgtca aaacccagcg attcgagccg cagggtctgc tgttcgccga tcagcgtgtg
     1261 cttgtcgaga atccgcagca ccgcatcctc gccgtggatg ctgggcatga tcgaaacgcg
     1321 gaagtcgacg gcgcggccct tgatcagcac cttgaaacgg ccgtcctgtg gcacgcgccg
     1381 ctcggaaatg tcgagctcgg ccatgacctt gatccgcgac agcacctggt cggagaggtc
     1441 tctgccctgg acgttgccga gcgtcgccag cacgccgtcg aggcggtact tgatggtcag
     1501 gccgttggtc ccggtttcaa tgtggatgtc gctggtgttg gcgcgcagcg catcgtagat
     1561 cgtcgagctg acgagcttga cggcagggct gtcgccctcg ctgatcgagg cgatcgacaa
     1621 ggcctcggtg ccgtcggcat cgacgccggc ggcgctctgc cccgactcga gcacgctgtc
     1681 cattgcccgc atcgactctt cctggcgcga cagcagcgcg cgcaggtcgg ccgaatacgt
     1741 gagccgccag tcgaagggtt cgccgatcgc ggtctcggcc cacagacgca gcgccgggtc
     1801 gaagggctcg ccgatcgcgg cgaggaggcg gccgtcagca tcgcgcagcg ccacacactc
     1861 gcgctgcgcg gcctgcgcgt agggcagcac gtcgaagtcg ggcgtcagcg cgtgcaactc
     1921 gtccatcgac aggctgtcga ggccgagcgt gcgtccgagg caggcgacga actcgggcgg
     1981 agagaggcca agccgggcct cgacgatatc gacgacgtga attccgctgg cggccgactc
     2041 ggccatcgct tccgcgatca tctcgcgcgg cacgcgcagt ggcgcttccc cgctccccga
     2101 ttgcgggggg gcttccccgc gggtttcagc agacaccacc gacgattcgg ccaccgcggc
     2161 agccgtagtc gctgcggctg gtaccggcgc agctggctca gcggtcggca cggcagccac
     2221 ttcgggcggg gcctcggcaa gcgtctcggt tgccggcaat gcgtcctgca gaagatcggc
     2281 ctgcgcctcg ctcacccgct tgccgcggcc atcgcggccg cggcgccgtc ccttggcggg
     2341 cggcgcggcg gcaccggcgt tggccggtcg atcgggctgg tcactggaca ttttcggcaa
     2401 gctcgaagat gggcatgtag aggaggacga ggatcacgcc gacgatcgcg ccgatgatga
     2461 tcatcagcgc aggttcgaag agaagcatga accagtccac ccagcgtgtc agttcatctt
     2521 cgtagaacga tgcgatgcga tcgaccattt cggccattcg gccgctgcgt tcaccgacgc
     2581 cgagcaggcg gcgggcgatc ggcgtcgtca ggccgtggcg ctcgaaggca cgcgacaccg
     2641 gcacgccctc gcggatctct cgggcggcgg ccttgagccg gtcgcgcagg accggcgaga
     2701 gcagcgcgct cgacatgtcg atcgaacgcg gcaggggcat tccgctgttg agcagcatcg
     2761 ccagcgtgcg atagaagcgg gccagctcgt agctgcgcag gcgctcgccg atgccgggac
     2821 cctgccacag gc
//
LOCUS       DATDAA010000284         3556 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_125981,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000284 DATDAA010000000
VERSION     DATDAA010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125981"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VIS73_08885"
     CDS             <1..417
                     /locus_tag="VIS73_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763311.1"
                     /translation="LLGARNNEPPVDEKDENVCWPMPGSGEIDIFEHHSDGGPEHYVA
                     RIIEDLGHCDGGDWETGQVALKADLTEYHEYSVEWREGDLVFARDGAEVYRLAGRADD
                     FAEPMFAILNFAKINDAPMEGEWVMEVDWVRHESLR"
     gene            448..1701
                     /locus_tag="VIS73_08890"
     CDS             448..1701
                     /locus_tag="VIS73_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEY5763312.1"
                     /translation="MNDKNGNAGLWAVLGPGLLVAATGIGAGDLATASFTGSILGTAV
                     LWAAVVGAFLKYVVTEGLARWQLATGDTLLEGLVHRIGRPVGWFFLPYLVIWSFFVAS
                     ALMSACGVTLHAMVPIFDDPKHGKVVFGALASLVGLGLVLAGGYELFDKVMRVCIGVM
                     FVTVIVTAVLLWPGTGQVLKGLVVPAIPDAGGQGLSWTVALMGGIGGTLTVLCYGYWI
                     REEGRTGGGFLKTCRIDLAAGYAITALFGVAMVIIGSTIEIEGRGATLLVRLSDQLVE
                     PLGQFGKWLFLVGAAGAVFSSLLGVWQAVPYLFADTLGLLREDGRASADAAIDTRSPA
                     YRGYLFALAIAPMVGLFWRFQDIQKLYAVIGATIIPFLAIALLVLNGRADWVGKRFRN
                     RPATVVVLLATLAFFLWIGWQSIWG"
     gene            complement(1753..2355)
                     /gene="amrA"
                     /locus_tag="VIS73_08895"
     CDS             complement(1753..2355)
                     /gene="amrA"
                     /locus_tag="VIS73_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEY5763313.1"
                     /translation="MSTQALHRHAPDPGKTRDAAGLELLRLARASIEHGLVYGRPLPV
                     DNDALPRALAAMAATFTTLRFQGQLRGCCGTLEPVRTLAEDVAYSAFRAAFRDVRFDP
                     VGKHELAAIRLEVSVLSPLEPIRFSREADLVGGLTPGIDGLVIIAEGRRATLLPKVWE
                     MLPDPQQFLAALKTKCGLSGTYWSERLEFQRYRTTSYAEE"
     gene            2598..>3556
                     /locus_tag="VIS73_08900"
     CDS             2598..>3556
                     /locus_tag="VIS73_08900"
                     /inference="COORDINATES: protein motif:HMM:NF004996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY5763314.1"
                     /translation="MQTDSKQLRPEPGGELASPRPDFEQAEAAWTSVPAEAAALQRIA
                     TDTHYPLLERVAAFEDLIDAEVGDTDLTRVRNIERETNLRQLYVKFEGGNPSGTQKDR
                     IAFAQAKDALRRGFDCMTLATCGNYGAATALAASLAGIRCIVYIPEEYAAKRLPEMTR
                     YNAEIVRVSGDYEAAVQASSDAASQRDWYDANPGGNNTALQLAAYGEIAREIYDDMRD
                     APAAVAVPVSNGTLLAGIYRGFLSLYRRDKTSRVPHMVAGSTYKQNPIIAAFLSGADH
                     CPDLDPAQIRETEINEPLVNWHSIDGDLALAAVRDSAGWADYAT"
BASE COUNT          626 a         1146 c         1157 g          627 t
ORIGIN      
        1 ttgctcggcg cccgcaacaa cgagcccccg gtcgacgaga aagacgagaa cgtctgctgg
       61 ccgatgccgg gctcggggga aatcgacatc ttcgaacacc acagcgacgg tggcccggag
      121 cattacgtgg ctcgcatcat cgaagacctc ggccactgcg acggcggtga ctgggagacc
      181 ggccaggtgg cgctgaaggc cgatctcacc gagtaccacg aatactcggt ggaatggcgg
      241 gagggagacc tggtctttgc gcgtgacgga gccgaggtct atcgtctcgc cggccgcgcc
      301 gatgattttg cggagccgat gtttgccata ctcaatttcg cgaagatcaa tgatgcgccg
      361 atggaaggcg aatgggtcat ggaagtggac tgggtacggc acgaatcgtt gcggtgaccg
      421 ggcgaccgag gacttcgcac ggcagctatg aacgataaga acggaaacgc cggcttgtgg
      481 gcggttctcg ggccaggcct cttggtggcg gcaacgggca tcggcgcagg cgacctcgcg
      541 accgcgagct tcacgggcag catcctgggc acggccgtgc tctgggcggc ggtcgtgggc
      601 gcgttcctga agtacgtggt aacggaaggc ctcgcgcgct ggcagctggc caccggggac
      661 accctgctcg aaggactcgt gcaccgcata ggccgcccgg tgggctggtt cttcctgcct
      721 tacctcgtca tctggtcgtt cttcgtcgcg tcggcgctga tgagcgcctg cggcgtgacc
      781 ctgcacgcga tggtcccgat tttcgacgac cccaagcacg gcaaggtcgt cttcggcgcg
      841 ctcgcgagcc tcgtcggcct gggcctcgtc ctcgccgggg gctacgaact cttcgacaag
      901 gtgatgcggg tttgcatcgg cgtcatgttc gtcacggtca tcgtgacggc cgtactgctg
      961 tggcccggca ccggccaggt gttgaagggc ctcgtcgtgc ccgcgatacc ggacgccggc
     1021 ggccagggat tgagctggac cgtcgcgctg atgggcggca tcggcggcac gctcaccgtg
     1081 ctctgttatg gctactggat ccgggaggaa ggccggacgg gcggcggatt cctgaaaacc
     1141 tgccgcatcg acctggccgc cggctatgcg atcaccgcgc tgtttggtgt cgccatggtc
     1201 atcatcggca gcacgatcga aatcgagggc cgcggcgcaa ccttgctcgt gcgtctgtcc
     1261 gatcagctgg tcgagccgct gggccagttc ggtaaatggc tgttcctcgt cggcgccgcc
     1321 ggcgccgtat tcagcagcct gctcggcgta tggcaggcgg tgccctacct gttcgcggac
     1381 acgctcggcc tgctgcgcga agacggaagg gcatcggcag atgcggcgat cgatacccgc
     1441 tcgccggcct accggggcta cctgttcgca ctggcgatcg caccgatggt gggtctcttc
     1501 tggcgattcc aggatatcca gaaactgtac gccgtcatcg gcgccactat catcccgttc
     1561 ctcgcgatcg cactcctggt cttgaacggt cgtgccgact gggtcggcaa gcgctttcgc
     1621 aaccggccgg cgaccgtcgt cgtgctgctc gccacgttgg cgttcttcct ctggatcggc
     1681 tggcagtcaa tttggggata gtggcttcaa ataccaggcg aatcatgagg cccgcctggt
     1741 gccaaagctc acctattctt ccgcgtacga cgtggtccgg taccgctgga actcgagccg
     1801 ctccgaccag tacgtccccg acaggccgca cttcgtcttt agcgcagcaa ggaactgctg
     1861 cggatcaggc agcatctccc agactttcgg cagtaacgtc gcacggcggc cttcggcgat
     1921 gatgaccagc ccatcgatgc ccggcgtcag gccgccgacc agatcggctt cacgtgagaa
     1981 gcgtatcggt tccagtggcg aaagcactga gacctccagc ctgatggcag ccagttcgtg
     2041 tttgccgacg gggtcgaagc gcacgtcgcg gaatgcggcc ctgaacgcgg agtaagccac
     2101 gtcctccgca agcgtgcgca ccggctcgag cgttccgcaa cagccacgca gctggccctg
     2161 gaagcgcagg gtagtgaaag tcgccgccat cgccgccagc gcccggggca atgcatcgtt
     2221 atcgaccggt aaaggccgcc cgtagacgag tccgtgctcg atcgaggcac gtgccaggcg
     2281 caacagctcc aagccggcgg catctcgggt cttgccggga tccggcgcat gccggtgcaa
     2341 ggcctgcgtg ctcatcgtcc cgcgattatc ggctcgatca tgctggtgta ttggggactg
     2401 aacactctat gtcaagaccg gccctccagg gggacagcga ccccaagcgg cgggcaattg
     2461 agaacattgt ctcctgattg cgatattgcg aataagcgaa gattcgtatt gcgccggccg
     2521 cgcttttgcg ccacgtttat ccggttattc ggtgtcgcag tcgaagcagc gtgctctgct
     2581 gctgatttga ggacacgttg cagaccgatt cgaaacaact ccgcccagag cccggtggcg
     2641 aattagcttc accgcggccg gattttgagc aagcggaagc ggcatggacc agcgtgccag
     2701 cggaagcagc cgccctgcag cgcattgcca ccgatactca ttatccgttg ctggaacgcg
     2761 tcgccgcgtt cgaggacctg atcgatgcag aggtcggcga cacggacctg acccgtgtcc
     2821 gcaacatcga acgcgagacg aacctgcgcc agctgtacgt caaattcgag ggcggcaatc
     2881 cgtccggcac ccagaaggac cgtatcgcgt ttgcgcaggc gaaagacgcg ctgcgccgcg
     2941 gcttcgactg catgacgctc gcgacctgcg gcaactacgg cgctgcgacc gcgctggccg
     3001 ccagcctcgc ggggattcgg tgtatcgtct acatccccga ggaatacgcc gccaagcggc
     3061 taccggagat gacccgctac aacgccgaaa tcgtgcgcgt ttccggcgac tacgaggcgg
     3121 ccgtgcaggc gtcgagcgac gccgcgagcc agagagactg gtacgacgca aatccgggcg
     3181 gcaacaacac cgccctgcag ctcgccgcgt acggcgagat tgcgcgcgag atttatgacg
     3241 acatgcgcga cgcgcccgcc gcggttgccg tgccggtgtc caacggcacg ttgctcgcgg
     3301 gcatctaccg cggcttcctg agcctctacc ggcgcgacaa gacgtcccga gtgccgcaca
     3361 tggtcgccgg cagcacctac aagcagaacc cgatcatcgc cgcattcctg agcggcgccg
     3421 atcactgtcc ggacctcgac cccgcgcaga tccgcgaaac cgagatcaac gaaccgctgg
     3481 tcaactggca ctcgattgac ggcgatctcg cgctggctgc cgtccgcgac agcgcaggct
     3541 gggccgacta cgccac
//
LOCUS       DATDAA010000285         2246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_510120,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000285 DATDAA010000000
VERSION     DATDAA010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510120"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VIS73_08905"
     CDS             complement(<1..418)
                     /locus_tag="VIS73_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763315.1"
                     /translation="MADRPLLRRNFFAGLAPALAVALVLPMEGARAQVEAQGYREPSQ
                     VLVNIVDAPPMPAVSASPDKEWLLLMQSPSLPPIADLAQRELRVAGTRIAPQTNGPSR
                     SRDYVDAWFKRVADGRETLFSGLPADPKLGDFAWSPD"
     gene            766..1974
                     /locus_tag="VIS73_08910"
     CDS             766..1974
                     /locus_tag="VIS73_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY5763316.1"
                     /translation="MGAGSLLGMVAVVWVIMPVVERWSSATLSVPLERVRVAEVVRAD
                     LVSDVSVQGRVVAAVSPTLYAPAPGTITLEVESGASVVEGQVLAEIYSPELANQLDQA
                     EAQLEQQSMELERQRIGARQEALEKRKAADLAEVARVAARREMRRADEAYERKVIPVI
                     DLEKAKDDLSNAELAFDHAKADADLFGERAEFEYKAGKLKLDRQQLLVDELRRQVEQL
                     SIRSPVSGIVGDLLVDQRAAISRDTPIMAVVDLSRFEIDALIPETYADDLAIGIDAEI
                     HVGSEIFRGRLVAVSPEIVDGQVRGRVRFGGAMPERLRQNQRLTTRLLLDEVHDVLTV
                     KRGQFLESGGGRVAYVVADDNIARRRGIETGGRSLSDVEIVAGLDEGERVIISSIDQF
                     NHADTVLLTD"
     gene            1996..>2246
                     /locus_tag="VIS73_08915"
     CDS             1996..>2246
                     /locus_tag="VIS73_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5763317.1"
                     /translation="MLQMKNIAKVYRTDAVETHALREFSLTVDEGEFVSVTGPSGSGK
                     TTFLNIAGLLEGYSAGAYELDGHDVSDLDDRQRSRLRNE"
BASE COUNT          423 a          724 c          760 g          339 t
ORIGIN      
        1 cgtccggcga ccacgcgaag tcgcccagct tcggatcggc gggcagaccc gagaagagtg
       61 tttcccgccc gtctgcgacg cgcttgaacc aggcgtccac gtaatccctg ctcctcgacg
      121 gcccgttcgt ctgcggcgcg atgcgggtgc cggcgaccct tagttcgcgc tgcgcgaggt
      181 ccgcgatcgg cggcaggctg gggctttgca tcaggagcag ccattctttg tcagggctgg
      241 cgctgaccgc gggcatcggc ggcgcatcga cgatattgac gagcacctgc gacggctccc
      301 ggtagccctg ggcctcgacc tgggccctgg cgccctccat gggcaggacg agcgcgacgg
      361 ccagcgccgg cgcaaggccg gcgaaaaagt tgcgtcggag caatggtcgg tcggccatga
      421 gatcacctcg attgagttga cgggacgaag agggcccgtc ggagcggcca gcatagccag
      481 tgcgggaagg gggttcaatc tgcaagaggc gcgccgggcg agctcccgtg cgcaccgagc
      541 gccgcccccg cgcagaatcg ccgcggaatc tcgagcccga tcccagccga atcgcgaccg
      601 cgttatcaaa tttcggtcgg gccgataaac gtctcggcgg aaacgcgcat ctaaaccaaa
      661 aacaggcaca tttcgcctgc cgcagggaga acgaagctgg ccatagaaga cacatccgcc
      721 caggacgtcg cgcttgcacc gcgcagcaag cgaacccgca acctcgtggg cgccggcagt
      781 ttgctgggaa tggtcgccgt cgtctgggtc atcatgcccg tcgtcgaacg ctggtccagc
      841 gcaacgctgt cggtgccgct cgagcgcgta cgtgtcgccg aggtggtgcg cgcggatctc
      901 gtgagtgacg tgtcggtgca gggcagggta gttgccgcgg taagcccgac cctctatgcc
      961 ccggcgccgg gcacgataac gctcgaggtc gagtccggcg cgagcgtcgt cgagggccag
     1021 gtgcttgccg aaatctacag cccggaactg gccaaccagc tggaccaggc ggaggcgcag
     1081 ctcgagcaac agtcgatgga actcgagcgg cagcggatcg gcgcaagaca ggaggcgctc
     1141 gaaaaacgca aggcggccga cctggccgag gtcgcccggg tcgccgcgcg gcgcgagatg
     1201 cggcgcgccg acgaggccta cgaacgcaaa gtcataccgg tcatcgacct ggaaaaggcg
     1261 aaggatgatc tcagcaatgc cgagctcgct ttcgatcacg caaaagcgga cgcggacctc
     1321 ttcggcgagc gcgccgaatt cgaatacaag gcgggcaagc tcaagctcga ccggcagcag
     1381 ctcctcgtcg atgagctaag gcgccaggtc gagcagctgt cgatccgctc gccggtcagc
     1441 ggcatcgtcg gcgacctgct ggtcgaccag cgcgccgcca tatcgaggga tacgccgatc
     1501 atggccgtcg tcgacctgtc gcgtttcgag atcgacgcgc tgattccgga gacttatgcc
     1561 gacgatctcg cgatcggcat cgacgccgaa atccacgtcg gcagcgagat cttccgcggc
     1621 cggctggtcg cggtttcgcc cgaaatcgtc gacggccagg ttcgcggccg ggtgcgcttc
     1681 ggcggcgcga tgcccgaaag gctgcgccag aaccagcgct tgacgacgcg actgctgctc
     1741 gacgaggtgc acgacgtgct gacagtgaaa cgcggccagt tcctggagtc gggcggcggc
     1801 cgcgtcgcct acgtcgtcgc cgacgacaac atcgccaggc ggcgcggcat cgaaaccggc
     1861 ggccgcagcc tgagtgacgt ggaaatcgtc gccgggctcg acgagggcga gcgcgtcatt
     1921 atttcgagta tcgaccagtt caaccatgcc gacaccgttc tgctgacgga ctgacggccg
     1981 cgaaggagat ttgaaatgct gcaaatgaag aacatagcga aggtgtaccg aaccgacgcc
     2041 gtcgagacgc acgcacttcg cgagttctcg ctgaccgtcg acgaaggtga gttcgtgtcg
     2101 gtcaccggcc cgtccggctc gggcaagacc acgttcctga acatcgccgg attactcgag
     2161 ggatactccg cgggcgccta cgaactggac ggtcacgacg tctcggacct cgatgaccgc
     2221 cagcgttccc ggcttcgcaa cgagaa
//
LOCUS       DATDAA010000286         4530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_405638,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000286 DATDAA010000000
VERSION     DATDAA010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405638"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VIS73_08920"
     CDS             complement(<1..760)
                     /locus_tag="VIS73_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEY5763318.1"
                     /translation="MTEAHQTPALLADVGGTHVRFAQLGKDGRPFAVRCLALADFDTF
                     EAALADYLAQSGARAITAAAIALAAPVGDGRRPVRLTNARWTIDPAALRTRFGWSRVE
                     LLNDFAALAMSLPYLPATELGQVCGGQAEVRGVRAVIGPGTGLGVASLVAAGRGWIAV
                     PGEGGHVTLPAADAREASIIAVARREHPHVSAERLVSGSGLPLLLRCVCAVDGLPLAP
                     ETMSPEAIVAAALGGDVPACVATVDTFLALLGTVA"
     gene            948..1283
                     /locus_tag="VIS73_08925"
     CDS             948..1283
                     /locus_tag="VIS73_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763319.1"
                     /translation="MMIDDAIPEQELRCEERRGFDEQPVGEFTIRIAERSVPVLALQD
                     VSSGGLRADVSCSIGISTPILLCYRRADIYVELNAITCWESPIADRPGEARTVGIELL
                     SPTILMNLL"
     gene            complement(1295..2524)
                     /locus_tag="VIS73_08930"
     CDS             complement(1295..2524)
                     /locus_tag="VIS73_08930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5763320.1"
                     /translation="MFPLLRYFSIASLISVIVTTVALGVLHHRIEREQLLKIGESNHV
                     ALTQSFANSLLPQFRRLAETARQVDIQSLPVHPEFSTMHSGVLDAMRNTQVVKVKVYD
                     LSGTTIYSTEAAQVGRDYSNNAGFLAARGGEPRSELTHRDRFSAFDREIVDRDVLSSY
                     VALQASGSDTAEGVLEVYSDVTEWIAHADQQARIITLAVIVSLCLLYTVLHIIVQRAD
                     RILRSQYAKQKKSEAEIRNLAFYDSLTRLPNRRLLQDRLQQAVVASTRTRQWGALLFV
                     DLDHFKTVNDHYGHEQGDSLLVQVAQRLSAGVREVDTVARWGGDEFIVVLEEIGDSRD
                     SAMRHAQAVAEKLLVAFQEPFMLGNEACRNTPSVGVTLFLGQEVPVGQLLREADAAMY
                     GAKSAGRNTVRVFGEKP"
     gene            2643..3080
                     /locus_tag="VIS73_08935"
     CDS             2643..3080
                     /locus_tag="VIS73_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763321.1"
                     /translation="MSILTNIVAAEDDEIEAVGEAEHPIDEWSGIEMRDIDTAKIVAL
                     HALLTGDLLDDAWANYEPIYVSAAEGAIVLRLADELAAKLAELDEDAVANVGAELAAT
                     EAFEAEGWDTDEITGMVDEMANLARLADSQCQALLIWMHPLRT"
     gene            complement(3104..3529)
                     /locus_tag="VIS73_08940"
     CDS             complement(3104..3529)
                     /locus_tag="VIS73_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEY5763322.1"
                     /translation="MESHLTDIARVIQLAVAPVFLLTALATMISALNTRLGRIVDRRR
                     VVFARSDSAADAESAGELDLLARRSRLVYFAIFCVVSAALLVCLVVAGAFLGALLSVD
                     LARMVAALFIGAMLAMIAGLSLFLREVYLAVRAGSHNRR"
     gene            complement(3580..>4530)
                     /locus_tag="VIS73_08945"
     CDS             complement(3580..>4530)
                     /locus_tag="VIS73_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5763323.1"
                     /translation="EADIAFAEVGTPTWDGLPLDRYLSVLDMLNPMHRLWSDGRWNKL
                     TVAHGCYWKKCSFCDVGLDYIGRFDGAPATLLVDRIEAVIAETGQTGFHFVDEAAPPK
                     ALRALAEELQRRNLAISWWGNIRFEKSFSPELCALLADSGCIAVSGGLEVASDRLLKL
                     MQKGVSVEQVARVTRAFSEAGILVHAYLMYGFPTQTVQDTVDALEYVRQLFDAGCISS
                     GFFHRFTCTVHSPVGLHPERYGITLEPLPPGGFARNDVRFTDPTGVDHEALGQGLTKA
                     LYNYMHGIGLDQDLRGWFPLRVPKTRVPRDFVARALAAAQ"
BASE COUNT          849 a         1516 c         1434 g          731 t
ORIGIN      
        1 cggcaaccgt tccgagcagc gccagaaagg tatcgacggt cgccacgcag gccggcacat
       61 cgccacccag cgccgcggca acgatagcct ccgggctcat cgtttccggc gccagcggca
      121 ggccatcgac tgcacagacg cagcggagca gcaagggcag gccgctgccg gatacaagtc
      181 gttcagccga aacatgcggg tgctcgcgcc gcgccaccgc gatgattgag gcctcgcgcg
      241 catcggctgc cggcagcgtc acgtggccgc cctcacccgg caccgcgatc catccgcggc
      301 cagcggcaac gagcgaggcg acgccgagtc cggtgccggg gccgatcacc gcccgcaccc
      361 cacgcacctc ggcctgcccg ccgcagacct gtcccagctc ggtggccggc aagtacggca
      421 gcgacatcgc aagcgccgca aagtcgttga gcagctcgac gcggctccag ccgaaacgcg
      481 tacgcagtgc cgcgggatcg atcgtccacc gggcattggt caggcgcacg ggccggcgcc
      541 cgtccccgac cggcgccgcc agcgcgatcg ccgccgccgt tatcgctcgt gcaccgcttt
      601 gcgcgaggta atcggcaagc gccgcttcga aagtgtcgaa atcggcgagg gcgaggcaac
      661 gcacagcaaa cggtcggccg tctttgccta gctgcgcgaa gcgcacatgg gtgccgccga
      721 catcggccaa cagcgctggg gtctggtgcg cctcagtcat ggagcaccgc ggcgatgcag
      781 cccctgcctg ttccggaatt gccgctggaa tacatgaggg atcgcccgga ccggacattg
      841 gaagcaccat tatgcttttt ttgccgcaac cgtgaagagt tcctcacgtc atagctggcg
      901 ggcgcttatc atcgccacgc ccacctagtc cgtgagaaag acgagcgatg atgatcgacg
      961 acgcgattcc cgaacaggaa ctgcgctgcg aggagcgacg cggcttcgac gaacagccgg
     1021 tgggcgagtt cacgatccgc atcgccgagc gcagcgttcc cgtgctggcc ttgcaggacg
     1081 tctcctcggg cggcctacgc gccgatgtct cctgctcgat cggcatctcg acgccgattc
     1141 tcctctgcta ccgccgcgcc gacatctacg tcgagctcaa cgcgatcacc tgctgggaat
     1201 cgccgatcgc cgatcgaccg ggagaggccc gcacggtcgg catcgaactg ctgagcccga
     1261 cgatcctgat gaacctgctc tagggcccgc ccgctcaggg cttctcgcca aacacccgca
     1321 cggtattccg cccggccgac ttggcgccgt acatcgcggc gtcggcttcg cgcagcaact
     1381 gccccaccgg aacctcctgc ccgaggaaca gcgtcacgcc gacgctgggc gtgttgcggc
     1441 atgcctcgtt gccgagcatg aacggctcct ggaaggcaac gagcagcttt tcggcaaccg
     1501 cctgcgcgtg gcgcatcgcc gaatcgcgcg agtcgccgat ttcctcgagg acgacaatga
     1561 actcgtcgcc gccccagcgg gctaccgtat cgacctcgcg gacgccagcg ctcaggcgct
     1621 gcgccacctg tacgagcagg ctgtcgccct gctcgtgccc gtagtggtcg ttgacggtct
     1681 tgaaatgatc gagatcaacg aacaacagcg cgccccactg gcgtgtccgc gtgctggcga
     1741 cgacggcctg ctgcaggcga tcctgcagca ggcggcgatt cggcagccgg gtcaatgaat
     1801 cgtaaaaggc cagattgcgg atttccgctt cggatttctt ctgttttgcg tactgcgagc
     1861 gcagtatccg gtcggcacgc tggacgatga tgtgaagcac tgtgtagagc aggcacaagg
     1921 aaacaatcac agccagcgtg atgattcgcg cctgttggtc ggcgtgggcg atccactcgg
     1981 tgacatcgct gtagacttcg agcacgcctt cggcggtatc gctgccgctg gcctgcagcg
     2041 cgacgtagct cgacagcacg tcgcgatcaa cgatttcgcg gtcgaaggcg ctgaagcggt
     2101 cgcggtgcgt cagctcgctg cgtggctcac cgccacgcgc cgcgaggaag cccgcattgt
     2161 tgctgtaatc ccggccgacc tgcgcggctt cggtggaata gatggtcgtg ccgctgaggt
     2221 cgtagacctt caccttgacc acctgcgtgt tgcgcatcgc atcgagcacg ccgctgtgca
     2281 tagtactgaa ttcgggatgc accggcagcg actggatatc gacctgcctt gccgtttcgg
     2341 ccagcctgcg gaactggggc aggagcgaat tggcaaagct ctgggtcagc gcgacgtggt
     2401 tgctctcgcc aatcttcaac agttgctcgc gctcgatccg gtggtgcagc acaccgagcg
     2461 ccacggtcgt gacgatgacc gaaatcaggc tcgcaatcga gaaatagcga agcagcggaa
     2521 acacgatgcc tcccggcgcg caaaatgcgc tcattatctt cccattgccc gctggggcgc
     2581 ggacaatccg cccggagcag cggatgccgc gcgacaacct tcacatcttt cggagaattt
     2641 ccatgtccat cctcaccaac atcgtcgccg ccgaagatga cgagatcgag gccgtcggcg
     2701 aagccgagca tccgatcgac gaatggagcg gcatcgaaat gcgtgatatc gataccgcca
     2761 agatcgtcgc cctgcacgcg ctgcttaccg gcgatctgct tgacgatgcc tgggcaaatt
     2821 acgaaccgat ctatgtctcg gccgccgaag gcgcgatcgt cttgcgcctc gccgatgaac
     2881 tggccgcgaa actggccgag cttgacgagg acgcggtcgc caatgtcggc gccgaactcg
     2941 ctgccaccga ggccttcgag gccgagggtt gggataccga cgagatcacc ggcatggtcg
     3001 acgaaatggc caacctcgca cggctcgctg actcgcagtg ccaggccctg ctgatctgga
     3061 tgcacccgct gcggacctga atcaagccag tccggggcgt tgatcagcgc cgattgtggc
     3121 tgccggcgcg aacggccaga tacacttcgc gcaggaacag gctcaggccg gcgatcatcg
     3181 ccagcatcgc accgatgaac agcgccgcga ccatgcgcgc cagatcgacc gagagcagcg
     3241 cgccgaggaa ggcgccggcg accaccaggc aaaccagcag tgccgccgag acgacgcaga
     3301 agatcgcgaa atagacgagg cggctgcgcc gcgccagcag atcgagttcg ccggccgatt
     3361 cggcgtcggc ggcgctgtcg ctgcgcgcga aaacgacccg ccggcggtcg acgattcgcc
     3421 ccaggcgcgt gttaagcgcg ctgatcatcg tcgccagcgc cgtcagcagg aagacgggcg
     3481 cgaccgcgag ttggatgacg cgggcaatgt cggtcagatg cgattccatg gtctaccttt
     3541 ctgattgcgc tggcggctac tgccgcttca ttggcgccat tactgtgccg cggccagcgc
     3601 ccgggcgacg aaatcgcgcg gcacgcgcgt cttcggcacc cgcagcggaa accagccgcg
     3661 caggtcctgg tcgaggccga ttccgtgcat gtagttgtac agcgccttgg tcagcccctg
     3721 gccgagcgcc tcatggtcga cgccggtcgg atcggtgaag cgcacgtcgt tccgggcgaa
     3781 ccctcctggc ggcagcggct cgagcgtgat gccgtagcgt tcggggtgaa gcccgaccgg
     3841 tgaatgcacg gtgcaggtaa agcgatgaaa gaagccagag gagatacagc ccgcgtcgaa
     3901 gagctggcgc acgtactcga gcgcatcaac agtgtcctgc acggtttgcg tcggaaagcc
     3961 gtacatcaag taggcatgga cgaggattcc cgcctcgctg aaggcacggg tgacgcgcgc
     4021 cacctgctcg accgagacgc ccttctgcat cagcttgagc aggcgatctg atgccacttc
     4081 cagcccgccc gagaccgcga tgcagccgga atcggccagc aacgcgcaca actcgggcga
     4141 gaacgatttc tcgaagcgga tattgcccca ccaggaaatc gccagattgc gccgctgcag
     4201 ttcctcggcc agtgcccgca gcgccttggg cggcgccgcc tcatcgacga aatgaaagcc
     4261 ggtctgcccg gtctcggcaa tcaccgcctc gattcggtcg accaggagtg tcgccggcgc
     4321 gccgtcgaaa cgaccaatgt agtcgaggcc gacatcgcag aaactgcatt tcttccagta
     4381 gcagccgtgc gccaccgtca gtttgttcca gcgaccgtcc gaccacaggc ggtgcatcgg
     4441 attgagcatg tcgagcaccg acaggtagcg gtccaacggc aacccatccc aggtcggcgt
     4501 gccgacctct gcaaacgcaa tatcggcttc
//
LOCUS       DATDAA010000287         2437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_335845,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000287 DATDAA010000000
VERSION     DATDAA010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335845"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VIS73_08950"
     CDS             <1..408
                     /locus_tag="VIS73_08950"
                     /inference="COORDINATES: protein motif:HMM:NF023327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HEY5763324.1"
                     /translation="NPYLHWIINRSYGDELPHALREEHFETIRANLDRLEWHRVGLHE
                     YLDRAANESIDRFNLSDVFEYLSEGESGLVFERIAEVGRPGGRLAYWNMLVPRHRPET
                     LAHRLRPLPELAAALHESARTFFYSDFVVEQVV"
     gene            405..1748
                     /locus_tag="VIS73_08955"
     CDS             405..1748
                     /locus_tag="VIS73_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763325.1"
                     /translation="MIAAILQEPWLGIAVVAALVVLLLAALTLLRRLARIGAETVRKL
                     FHTGTGVVALSLPHLFDTLWPVVSICAAIMVGLVLIRVVPALEKGPGQVLQAVSRKSF
                     GEFWFVLGVGLAFAIAGRDPVVYSVGILTLAVADATAALVGVVYGRHRFDVVGGTKSA
                     EGSIAFFLVAFLCVHIPILLFTDTGRPESLLMAFNAGLLVMFAEASAARGADNFVLPV
                     LVVVLLDVFAEMTAAGLMLHAAVIVALGVLIAGYKRRTTLSGDALIGAVLAAYVCWSF
                     GGWRWLVAPLTLFFTYTWLVGRPQLESDETFHADVLLAIVAPGVVLVTAYAELGNPAL
                     YAPFVTAWSANLAVIGTFHGQLRDPDSPALREALVNSLKALVVLVPGLLAAGQLTLAE
                     ALAAVASVPTALLLFALIGRRLRDAPTSAGAWSAVPFSIGIAIAACFGLCAALGA"
     gene            complement(1753..>2437)
                     /locus_tag="VIS73_08960"
     CDS             complement(1753..>2437)
                     /locus_tag="VIS73_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763326.1"
                     /translation="GPVAFNTWFPYRFRLADGEPRIFDWEPCNPPDYVAAVENAGFRI
                     DERYNSTAFADLEAVASRFEPDYEKALQEGFRFEPFGTASAGALIPTLYRLSHAAFVD
                     NYLFEPIPEALFADAYIGLAGKGRPVRGWLLSMPRGEAAGFLYTFIDREGGETIAVLK
                     TIGVVPEMRGRRLSNALVCVALRDAVAAGVDYAVGALVHTGIQSESYAVKGSFLWRHE
                     YAIWRKEPA"
BASE COUNT          336 a          825 c          818 g          458 t
ORIGIN      
        1 aatccctacc tgcactggat cataaatcgt tcctacggcg acgagctgcc gcacgcgctt
       61 cgcgaagagc acttcgagac catccgcgcc aatctcgatc ggctcgagtg gcatcgcgtc
      121 ggcctgcacg agtacctgga ccgggcggcg aacgaatcga tagatcgctt caacctgtcg
      181 gacgttttcg aatacctctc cgagggcgaa tccggcctgg tcttcgagcg gatcgccgag
      241 gtcggtcgcc ccggcggccg gctggcttac tggaacatgc tggtgccgcg ccaccggccg
      301 gagacgctgg cgcatcggct tcgcccgttg cccgagctcg cggcggcgct gcacgagtcc
      361 gcgcggacct ttttctacag cgacttcgtc gtcgagcagg tcgtgtgatc gccgcgatcc
      421 ttcaagagcc ctggctcggc atcgccgtcg tcgccgcgct cgtggtcctg ctccttgccg
      481 cgctcacgct tttgcgccgg ctggcccgca taggcgccga aaccgtgcgc aagctgttcc
      541 ataccggcac cggcgtggtc gccttgagcc tgcctcacct gttcgacacg ctgtggccgg
      601 tcgtctcgat ctgcgcggcg atcatggtcg ggctggtcct gatacgggtc gttcccgcgc
      661 tcgagaaagg cccgggccag gtgctgcagg ccgtgtcgcg gaagtcgttc ggcgagttct
      721 ggttcgtgct cggcgtcggc ctggccttcg cgatagcagg gcgtgacccg gtcgtctaca
      781 gcgtcggcat tctcacgctc gccgtggcgg atgcgacggc ggccctggtc ggcgtcgtct
      841 acgggcgcca ccgcttcgac gtcgtcggcg gcacgaagag cgccgagggc tcgatcgcct
      901 tctttctcgt tgcattcctg tgcgtgcaca tcccgatcct gctgttcacg gacaccggcc
      961 gcccggagtc cctgctgatg gccttcaacg ccggcctgct ggtcatgttc gccgaggcga
     1021 gcgccgctcg cggcgccgac aacttcgtgc tgccagtgct ggtggtggtg ctgctcgacg
     1081 tgttcgcgga aatgaccgcc gccgggctca tgctgcacgc cgcggtcatc gtcgcgctcg
     1141 gcgtcctgat tgccggctac aaaaggcgga cgaccctgtc gggggatgcg ctgatcggtg
     1201 cggtcctggc ggcctacgtt tgctggagtt tcggcggctg gcgctggctg gtcgcgccgc
     1261 tgacgctgtt ctttacctat acctggctgg tgggccggcc gcagctcgaa tccgatgaaa
     1321 catttcacgc cgacgtgctg ctcgccatcg tcgccccggg ggtggtcctg gtgacggcct
     1381 acgccgaact cggcaatccg gcgctttacg cgccgttcgt gaccgcctgg agcgctaacc
     1441 tcgccgttat cggcaccttt cacggccaat tgcgcgaccc cgacagcccg gccctgcgcg
     1501 aagccctggt caacagcctg aaagccctcg tggtgctcgt ccctgggctg cttgccgccg
     1561 gccagctcac gctcgccgag gcgctggcgg cggtcgcgag cgtccccacg gcgctgttgc
     1621 tgttcgcgct catcggcagg cggctgcgcg acgcgcccac gagcgccggg gcctggagcg
     1681 cggtgccgtt ttcgatcggc atagcaatcg ccgcctgctt cgggttgtgc gctgcgctgg
     1741 gcgcctgagg cttcaggccg gttcctttct ccagatggcg tattcgtgcc gccacaggaa
     1801 gctgcctttg acggcatagg actcgctctg tatgccggtg tgcacgaggg cccccacggc
     1861 atagtcgacg cctgccgcga cggcgtctct cagcgcaaca cagacgagcg cattcgacag
     1921 ccgccggccg cgcatctcgg ggacgacgcc gatcgtcttc aggacggcga tcgtttcccc
     1981 gccctcccga tcgataaagg tatagaggaa gcccgcggcc tcgccgcggg gcatggacag
     2041 aagccagccg cggacgggcc ggcccttgcc ggccaggccg atatacgcat cggcgaacag
     2101 cgcttcaggt atcggctcga acaggtagtt gtccacgaag gccgcgtggc tcaggcggta
     2161 gagcgtcggg atcagggcgc cggcggacgc cgtgccgaaa ggctcgaagc ggaagccttc
     2221 ctgcaaggct ttttcgtagt cgggctcgaa gcgcgaggcg accgcctcga gatcggcgaa
     2281 cgcggtgctg ttgtatcgtt cgtcaatccg gaagccggcg ttttcgaccg cggcgacgta
     2341 gtccggcggg ttgcagggtt cccagtcgaa aatgcgtggc tcgccgtcgg cgagcctgaa
     2401 gcggtacgga aaccaggtgt tgaacgcgac cggcccc
//
LOCUS       DATDAA010000288         2706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_91252,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000288 DATDAA010000000
VERSION     DATDAA010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91252"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VIS73_08965"
     CDS             <1..332
                     /locus_tag="VIS73_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763327.1"
                     /translation="VLVSRKLHTASELHRPGHAGVANAIGAANAQVGSEGERIVSYRR
                     TPRETVIEEMCADLRQRLLKAGARAGTVKIADVEETAVSYMADDSTRIRVKMVGDLAL
                     SAKERGR"
     gene            329..1420
                     /locus_tag="VIS73_08970"
     CDS             329..1420
                     /locus_tag="VIS73_08970"
                     /inference="COORDINATES: protein motif:HMM:NF044937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HEY5763328.1"
                     /translation="MSVTITHEDVADLCAGSVFLATGGGGDPYVSRLLVEEALKKYGP
                     VGLVPLREVPDDACVVAIGEVGAPTVSLEQLPIGIESLQVVDRFEEWTGRKITHLVSF
                     EVGGANSVIPLIASAAKGIPLIDGDGMGRALPEAQMMTFAIEGVSPCPAVAVDYTGGA
                     VYFDTKDTLLYERQVRNVAMAMGGMIFTAEHPMTGAEARRAVVPGTISFALRLGRLLS
                     ANRGSAVVAEAPLAELFAASDYGVLKRLYTGKIVDIRRKVAGGFDVGEAVLEAVGADL
                     PPMTLSIKNEFLVAKVGDRVVASVPDLITMVDHETSTPINSERVHYGQRVTVFGIGCP
                     PHYRTERALGVVAPRAFGFDFDYLPIENL"
     gene            complement(1462..>2706)
                     /locus_tag="VIS73_08975"
     CDS             complement(1462..>2706)
                     /locus_tag="VIS73_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY5763329.1"
                     /translation="LVTILSGCMVPDAAYPLCRKAVELDPYNAAAHRRLALTCRLIGR
                     LDEAAEAAGIALRFDAHDYEMIGLRSSLGKATAEDNHVVELETLLAAGCRNQLGAARV
                     AYALAKELEDLGRHDRAFKFLDAGAEFKRKTIKYDVGADLRMMRKVREVFTTDAMAAA
                     SPGHDSEEPIFILGLPRTGSTLLERVLSSHSKVYAAGELNHFSAAMMEHIRSLGPIRD
                     QADLIEKSLLVDHRAVGERYIARSRPFTGHTPRFIDKLPGNFMTLGPIHLSLPKASII
                     HVRRAPIDACYAIYKFLFNEAYAWSYNLEELAKYYLAYHKLMAHWREVLPGRIVEIAY
                     EDMVGDLEPAARKLVGDLGLDWEDACLQFHRNEAAAMTGSAAQVRQKIYASSVGRWKD
                     YKSELRPVIKALKKGGVDPYTP"
BASE COUNT          432 a          852 c          942 g          480 t
ORIGIN      
        1 cggtgctcgt gtcccgcaag ctccatacgg cttccgagct ccaccggccc gggcacgccg
       61 gggtcgccaa cgccatcggc gcggcgaacg cgcaggtggg cagcgaaggc gaacggatcg
      121 tctcctaccg gcgtacgccg cgcgagacgg tgatcgagga gatgtgtgcc gacctgcggc
      181 agcggctttt gaaggccggc gcgcgggccg ggacggtaaa gatcgccgac gtcgaggaga
      241 cggcggtgtc ttacatggcc gacgattcga cgcgcatccg cgtgaagatg gtcggcgacc
      301 ttgccttgag cgcgaaggag cggggccgat gagcgtgact attacccacg aggacgtcgc
      361 cgatctctgc gccggctcgg tgttcctggc cacgggcggg ggcggcgatc cctatgtgag
      421 ccggctgctc gtcgaagagg ccctcaagaa atacggcccg gtcggtctcg ttccgctgag
      481 ggaggtgccg gacgatgcct gcgtcgtcgc catcggcgag gtcggcgcgc cgaccgtgag
      541 tctcgagcaa ttgccgatcg ggatcgaaag cctgcaggtc gtcgatcgct tcgaggagtg
      601 gacaggccgc aagatcacgc acctggtttc tttcgaggtg ggcggtgcca actccgtcat
      661 cccgctgatc gcctccgccg cgaagggcat cccgctgatc gacggcgacg gcatgggccg
      721 cgcgctgccc gaggcgcaga tgatgacctt cgccatcgag ggcgtgagcc cgtgcccggc
      781 ggtcgccgtc gattacaccg gtggcgccgt gtatttcgac acgaaggaca cgttgctcta
      841 cgagcgccag gtcagaaacg tggcgatggc catgggcggc atgatcttca cggccgagca
      901 cccgatgacc ggcgcggagg ccaggcgggc cgtcgtgccc gggacgatca gcttcgccct
      961 gcggctcggc cgcctgctgt ccgctaacag gggcagcgcc gtcgtggccg aagcgccgct
     1021 cgccgagctc ttcgccgcgt cggactacgg cgtcctcaag cgcctctaca ccggcaagat
     1081 cgtcgacatc cggcgcaagg tcgcgggcgg cttcgacgtg ggcgaggcgg tgctcgaggc
     1141 ggtgggcgcc gacctgccgc cgatgacgct gtcgataaag aacgagttcc tggtcgcgaa
     1201 agtcggcgac cgcgtggtcg cctcggtgcc cgatctcatc acgatggtcg atcacgaaac
     1261 ctccaccccg atcaattcgg agcgggtcca ttacggtcag cgggtcacgg tgttcggcat
     1321 cggctgcccg ccgcattacc gcacggagag ggcgcttggg gtcgtcgcgc cgcgcgcatt
     1381 cggcttcgat ttcgattacc ttccgatcga aaatttataa agcgcacccg ggagggcgca
     1441 tataaccagg cgatctttaa ctcacggcgt gtagggatcg acgccgcctt ttttcagcgc
     1501 cttgatgacg ggccggagtt ccgacttgta atccttccag cggccgaccg acgaggcgta
     1561 aatcttctgc cggacctgtg cggcgctgcc ggtcatcgcg gccgcctcgt tgcggtggaa
     1621 ctgaaggcag gcgtcttccc agtcgaggcc gaggtcgccg accagtttgc gggccgccgg
     1681 ctcgaggtcg cccaccatgt cttcgtaggc gatctcgacg attctgccgg gaagcacttc
     1741 gcgccagtga gccatcagct tgtggtaggc gaggtagtac ttggccagtt cctcgaggtt
     1801 gtaggaccag gcataggcct cgttgaacag gaacttgtaa atcgcgtaac aggcgtcgat
     1861 cggcgcccgg cggacgtgga ttatactcgc cttcggcagc gagaggtgga tgggccccag
     1921 ggtcatgaag ttgcccggca gcttgtcgat gaatcgcggc gtatggccgg taaacggccg
     1981 cgagcgggcg atgtagcgtt cgccgacggc gcgatgatcg accagcaggc tcttctcgat
     2041 caggtcggcc tggtcgcgaa tcgggccgag gctgcggatg tgctccatca tcgccgccga
     2101 gaagtggttg agttcgccgg ctgcgtatac cttcgagtgg ctcgacagca cccgctccag
     2161 cagggtcgag ccggtgcgcg gcaggcccag aatgaatatc ggctcctcgc tgtcgtggcc
     2221 gggcgaggcc gccgccatgg cgtccgttgt aaagacttcg cgcaccttgc gcatcatgcg
     2281 caggtcggcg ccgacgtcgt acttgatggt cttgcgcttg aattcggcgc cggcgtcgag
     2341 gaatttgaag gcgcggtcat ggcgtccgag gtcttcgagt tccttggcga gcgcgtaggc
     2401 gacccgcgcg gcccccagct ggttccggca gcccgcggca agcagcgtct ccaattcgac
     2461 gacatggttg tcttccgccg ttgctttccc gagggaggac cgcaggccga tcatctcgta
     2521 gtcgtgcgcg tcgaagcgga gcgcgatacc ggccgcttcg gcggcctcgt cgagtctgcc
     2581 gatcaggcgg caggtcagcg ccagccgcct gtgcgccgcg gcgttgtagg ggtccagttc
     2641 gacggccttc ctgcaaagcg ggtacgcggc gtcgggcacc atgcagcccg acagaatcgt
     2701 cacgag
//
LOCUS       DATDAA010000289         8197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_161187,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000289 DATDAA010000000
VERSION     DATDAA010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8197
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161187"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VIS73_08980"
     CDS             complement(<1..558)
                     /locus_tag="VIS73_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY5763330.1"
                     /translation="MKQHDLSRWTHGHRFDSGNPAGERGTRAVLWITLVAMVVEIVAG
                     LSFNSMALLADGWHMSSHALAIGLSVFAYAAARRYAGDQRFAFGTWKIEILGGYTSAI
                     LLLAIAALMVAGSVERIISPQPIAYREALLVAVLGLAVNLVCAVILGRAHGHDHGHPG
                     HGHHAHGEAHHHGHDLNLRSAYIHVI"
     gene            803..931
                     /locus_tag="VIS73_08985"
     CDS             803..931
                     /locus_tag="VIS73_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763331.1"
                     /translation="MLWAGKFNELVEAIREGVAYVKVHTTSASGGVASGELRVQIR"
     gene            complement(1035..1625)
                     /locus_tag="VIS73_08990"
     CDS             complement(1035..1625)
                     /locus_tag="VIS73_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5763332.1"
                     /translation="MNYDYTRFLLRGWIFHFFGRQRTAYAAYAEAFRINGNSAHAAKA
                     LGYIDAQRKHYHQAVGWYREAVRIAPDDAGNWYNLGYVLAEDGFRQEAAEIFRKAVAL
                     SPEMDRAWYGMGLALAHEGDHAGAAEAFQQAADRQPMHGAVWYHLGMARLYCCQPEHV
                     EKAIRHLAEFDPRTCHRLIVDTDRSDLEHLVHDRLL"
     gene            1702..2007
                     /locus_tag="VIS73_08995"
     CDS             1702..2007
                     /locus_tag="VIS73_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763333.1"
                     /translation="MDIATQVLLIKLVRALVEVAGFCLLGQGAVAIFAGPRRESNPIY
                     QLFRVVTRPVIALLRAITPKFIIDRHLPYLAFFILFWAWILLALLRVSICRANGLTC"
     gene            2088..2178
                     /locus_tag="VIS73_09000"
     tRNA            2088..2178
                     /locus_tag="VIS73_09000"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2122..2124,aa:Ser,seq:gga)
     gene            2378..3991
                     /locus_tag="VIS73_09005"
     CDS             2378..3991
                     /locus_tag="VIS73_09005"
                     /inference="COORDINATES: protein motif:HMM:NF015894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HEY5763334.1"
                     /translation="MPIAEISLVEDQARAVILSWQPAGEVAPVSEEQIVAELEAAGFA
                     QLWRDSTAIQQAAHRISQGASYSGVVARRVDAQYKVAVAPNKLVAALAVLAAQGGKAA
                     SVDDARAALAKAGVVHGVEEEKLAAAVAAASGTPVEVAQGTPPQHGKDGWLEPLVDVN
                     VNRFPKIDDKGHVDFRDMGVIPTVQVGDALMRRHPPTAGVKGVSVLGAEVPAVDGKDI
                     KFAVRLQGVVADPQDADLLRAEVVGQPVLLHDGVTVEPVLRLETVSVESGNVSFGGSV
                     IIKGDVQTGMKIVVDGDVTVEGVIESAEISAGGNVLARGGIIGNAPQTAQGAQSSTHT
                     AKITAKGNVTTRYAENALIVAEQSVIIDESLVQCDVTAVDRVQVGKPGKRKGHILGGF
                     VRATLGVDAEYLGGAGSGQTRVFVGANPLLQQAIDAQKKRVAGKLAEHGEISKVLKIL
                     KARPDRKEMFDKARATLMKISEEIAEAMAEEKRLVAQLGHADEAEIRVSEAITAGTMV
                     AIGKKSRYVTEDGRGASFRLSGGEIVSGD"
     gene            complement(4010..4879)
                     /locus_tag="VIS73_09010"
     CDS             complement(4010..4879)
                     /locus_tag="VIS73_09010"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY5763335.1"
                     /translation="MSLFGASKLPPISPFAQELFSLSIEADDAEQRLLRICRSEPELS
                     IRIVAVANSVVYRRGGAEVLEPLQAIRRVGLARTKQLATAMLFGQKLAGYLAPGLAEE
                     IWLHSLTMAAAAQEIAQTKGHPDPSAAYLVGLIHDLGHLVAEVGKAGSIAQAVSSAVT
                     NKLPLDKAEDKEWETNHARQAVELLTLWHAPANIVQVIGEHHQENADPNDLPAFVAGA
                     EEIARFAGIAERVHGGRADPFAPLAIDAQTLSTTLAQRLALKPEQIAVIAKRIVDQVE
                     GFQEAARALAGKR"
     gene            5143..6264
                     /locus_tag="VIS73_09015"
     CDS             5143..6264
                     /locus_tag="VIS73_09015"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5763336.1"
                     /translation="MDEGASGQWLGIGWRSRHAMHRDFLATDRSTSIGPDAFTGIAGM
                     AERAYDVPRTNFVGCMRGGAALTPQVGRFYPPAFLQGLQTRRPFRVLAVSPDTLRIDP
                     SPALAGRALEALSVPIIPGVAVRKWAAASDPLAVLKRAGVGFQGAANETETEFGLAMR
                     LLRADDGFDASFYSAPRLVHHLDAAARLLLADCNARLLGGLPDGAPVLDLMSSWTTHL
                     PAESGLTLTGLGMNAAELAANPLLTERVLHDLNEQPCLPFPNRHFSAAICSLSVEYLV
                     EPLAVFAELARVLRPGAPVVLSWTERWFPPKAVSIWSELHPYERVAMVVDCLRLALGW
                     HDIVTESYRGLMRPTDDRYADQLPEADPLYLVRALSVSR"
     gene            complement(6272..>8197)
                     /locus_tag="VIS73_09020"
     CDS             complement(6272..>8197)
                     /locus_tag="VIS73_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763337.1"
                     /translation="LRWAEIAAQTNTELGYLVAMLAPQLLARLDTDGSEALILAALER
                     YDQSGGRAAMEMLRDIEGFRLRRERGNAVARLELVESRLERLLHGLSGRRLRIEEAGD
                     GRAWTDTEVVYLPSAIADAYKHADNIAHYRVRAAILWGQTRFGTFNGDADATLARWHD
                     GDAARRWFAVLEAARIEAAIAEELPGLAAEIAALRGPWPDTVAQRIAPLAAGASLAGT
                     LHALDALRDDGEPTPPELPHAGAIDLAAALRVRAERIARETEILRRALAAHAGRPGAI
                     RPPAGTDQSAEPLPFALFAAGEIAALPPQAAAAARSLMQDLGEIPPELLSPAGDAPWA
                     PTTIAEPGASFDYGARPADYTYDEWDYRRNAYRSGWCHLFEHTVREGDADYVPQVRHR
                     YRAQIAQLRRRFEMLRGENRVLRQQPDGEEIDLDALIAARADRRAGTPASANLYSQRT
                     RNDRSLAVMLMIDMSGSTKGWVNDAEREALVMLGEAVEALGDSWAIYGFSGWTRTRCD
                     IFRVKAFAEAYDESVQRRIAAIEPKDYTRMGVAIRHLTRDLIRQPTRHHLLITLSDGK
                     PDDFSDEYRSRYGIEDTRRALLEARAQGVRSYCVTIDRNGADYLRHLYGPARYTVLDD
                     VKTLPLKIADIYRRLVT"
BASE COUNT         1334 a         2545 c         2889 g         1429 t
ORIGIN      
        1 gatgacgtgg atgtaggccg agcgcaggtt gaggtcgtgg ccgtggtgat gcgcttcgcc
       61 atgcgcgtga tggccatgcc cgggatgccc gtggtcgtgc ccgtgcgcgc ggccgaggat
      121 cacggcgcag acgaggttga ccgcaaggcc gagcacggca accagcagcg cctcacggta
      181 ggcaattggc tgcggcgaga tgattcgctc gaccgagccg gccaccatca gcgccgcaat
      241 cgcgagcagc aggatggcgc ttgtatagcc gccgagaatt tcaatcttcc aggtgccgaa
      301 ggcgaaacgc tggtcgccgg catagcggcg cgctgcggca taggcgaaca ccgaaagtcc
      361 aatcgccagc gcgtgcgaac tcatgtgcca gccatcggcg agcagcgcca tcgagttgaa
      421 ggacagccca gcaacgatct cgacgaccat cgccaccagc gtgatccaca gcacggcgcg
      481 cgtgcctcgc tcgccggccg gattgccgga gtcgaagcgg tgcccgtggg tccagcggct
      541 gaggtcgtgc tgcttcatgc gcggaggtcg agaggtggcg acagtgggga ttgtagccag
      601 caccggacca gatgccggaa tggggcaggg caatcgtgac cgagctgagc cccaccgcga
      661 gagggcggca ccatacagct ctcgatcccg gcggacgcga caacgacaac agcggtgtgg
      721 gcggcggaac gaatccggtg acgtcccggc caaccgggtt ggtgggagac cacgattccg
      781 cgatgccggc gcgcccgggc agttgctctg ggccggcaag ttcaacgagc tagtcgaggc
      841 gatccgcgaa ggggtggcct acgtaaaagt ccacaccact tcggcttcgg gtggtgtcgc
      901 cagcggcgag ctgcgcgtgc agattcggta ggtgccgcaa gcgggcacaa actcgaacct
      961 ggtcgcaggg tcggacgacg cggtgacggc gttcggcccc gcgccattta cgggtcgaat
     1021 tctccggagc cgtcctacag caagcggtcg tgaacgagat gctcgagatc gctgcgatcg
     1081 gtgtcaacga tcagccggtg gcaggtgcgc gggtcgaact cggccaggtg gcggatggcc
     1141 ttttctacgt gctcgggttg gcagcaatac agccgcgcca tgcccaggtg gtaccagaca
     1201 gcaccgtgca tcggctggcg gtcggccgct tgctggaagg cctcggcggc accggcatga
     1261 tcgccttcat gggcgagggc gaggcccatc ccgtaccagg cgcgatccat ttccggtgac
     1321 agcgcgactg ccttgcggaa gatctcggcc gcctcctgcc gaaaaccgtc ttcggccagc
     1381 acgtagccaa ggttgtacca gttccccgcg tcgtcgggcg cgatgcgcac cgcctcgcga
     1441 taccagccga ccgcttggtg gtaatgcttg cgctgtgcat cgatatagcc gagcgccttg
     1501 gccgcgtgcg ccgaattccc attgatccgg aacgcctctg cataggccgc gtaggccgtg
     1561 cgttggcggc cgaagaaatg aaagatccag ccgcggagca ggaatcgggt gtagtcgtag
     1621 ttcatcgttg ggtctttcgg cggttccgct agaatcgccg gcccgccggg tcggttccgg
     1681 ccgattgtat gcgagctgcc catggatatc gcaacgcagg tgctgctgat caagctggtc
     1741 cgcgcgctgg tcgaggttgc cggcttctgc ctgctcgggc agggcgcggt cgccatcttc
     1801 gccggccccc ggcgcgagtc gaatccgatc taccaactct ttcgcgtcgt cacccgcccg
     1861 gtgatcgcct tgctgcgggc aattacgccg aaattcatca tcgatcggca cctgccgtat
     1921 ctcgccttct tcattctctt ctgggcctgg atactgctcg cgctgctgcg agtgagcatc
     1981 tgccgcgcca acggcctgac ctgctgaacc ccgccgaccc gagtttcgct ataatcgcgc
     2041 ccttcgccga aacggcagca ctgccgggca ggcgattgca gcattgcgga gagatggccg
     2101 agtggtcgaa ggcgcacgcc tggaaagtgt gtatacggca aaaccgtatc gagggttcga
     2161 atccctctct ctccgccagt aacaccgata caacgcgccg ctcggcgcgt ttttcttttc
     2221 ttcctttgtg ttcgcctgct cgctctccgc catgccagcg ggcgcctctg cggccatatg
     2281 cgatagctcg atgctaggat gcggcgcaaa cgccgggccc gtcggcgaaa tgcctaggcc
     2341 ttcagaatta ccagtgacag aacgcgaagg tcagaagatg ccgatagcgg aaatcagtct
     2401 cgtcgaagat caggcgcgag cagtcattct ctcttggcaa ccggccggcg aggttgcgcc
     2461 ggtcagcgag gaacagatcg tcgccgaact cgaggcggcc ggcttcgcgc aactctggcg
     2521 cgattcaacg gcgatccagc aggcagcgca ccggatcagc cagggcgcca gctacagcgg
     2581 cgtcgtcgct cgccgcgtcg atgcccagta caaggtggcg gtggcgccga acaagctcgt
     2641 tgcggcgctg gcggtgctgg cagcgcaggg tggcaaggcc gcgtcggtag acgatgcgcg
     2701 cgcggcgctg gccaaggccg gcgtggtgca cggcgtcgaa gaggagaagc tggcggccgc
     2761 cgttgccgca gccagtggca caccggtaga ggtcgcgcag ggcacgccgc cgcagcacgg
     2821 caaggacggc tggctcgagc cactggtcga tgtcaacgtc aatcgcttcc ccaagatcga
     2881 tgacaagggt cacgtcgact tccgcgacat gggcgtgatt ccgaccgtgc aggtcggcga
     2941 tgcgctgatg cgccggcatc cgccaaccgc cggcgtcaag ggggtcagcg tgctgggtgc
     3001 agaggtgccg gccgtcgacg gcaaggacat caagtttgcc gttcgcctac agggcgtcgt
     3061 cgccgatccg caggacgccg atctgctgcg cgccgaggtg gtcggtcaac cggtcctgct
     3121 gcacgatggc gtgacggtcg agccggtgct acggctggag acggtcagcg tcgaatcggg
     3181 caacgtcagt ttcggcggca gcgtgatcat caagggcgat gtgcagaccg gcatgaagat
     3241 cgtcgtagac ggcgacgtca cggtcgaggg cgtcattgag tcggccgaga tttccgctgg
     3301 cggtaacgtg ctcgcgcgcg gcggcatcat tggcaacgcg ccgcagacag cgcagggcgc
     3361 gcagtcgtcg acgcacacgg cgaagatcac cgcgaagggc aacgtgacga cgcgctacgc
     3421 cgagaacgcg ctgatcgttg ccgagcagtc ggtgatcatc gacgagtcgc tggtccagtg
     3481 cgacgtgact gccgtcgacc gtgtgcaggt gggcaagccg ggcaagcgca agggccacat
     3541 cctcggcggc ttcgtgcggg cgacgctggg tgtcgacgcc gagtatctgg gcggcgccgg
     3601 cagcgggcag acgcgggttt ttgtcggcgc caacccgctg ctgcagcagg cgatcgacgc
     3661 gcagaagaag cgcgtcgccg gcaagcttgc cgagcacggc gagattagca aggtgctgaa
     3721 gatcctcaag gcccgccccg atcgcaagga gatgttcgac aaggcgcggg cgacgctgat
     3781 gaagatttcc gaggaaatcg ccgaggcaat ggccgaggag aagcgtctgg tggcgcagct
     3841 tggccacgcc gacgaggcgg aaatccgcgt gtccgaggca atcaccgccg ggaccatggt
     3901 cgcgatcggc aagaagtcgc gctatgtgac cgaagacggt cgcggcgcga gcttccggtt
     3961 gagcggcggc gaaatcgtca gcggcgactg aaggccgcta tttcgccggt cagcgtttgc
     4021 cggcgagtgc ccgcgcggct tcctggaagc cctcgacctg atcgacgatg cgcttggcga
     4081 tcaccgcgat ctgctcgggc ttaagcgcca ggcgctgcgc cagcgtggtc gacagcgtct
     4141 gcgcgtcgat cgccagcggc gcgaaggggt cggcgcggcc accgtgcacg cgttcggcga
     4201 ttcccgcgaa gcgggcgatt tcctcggcgc cggcgacgaa ggccggcagg tcgttggggt
     4261 cggcattttc ctggtgatgc tcgccgatca cctgaacgat attggccggt gcatgccaca
     4321 gcgtcagcag ctctaccgcc tggcgcgcgt ggttggtttc ccactccttg tcctctgcct
     4381 tgtccaatgg cagcttgttg gtgaccgccg atgaaaccgc ttgcgcgatg ctgccggcct
     4441 tgccgacttc ggcaacgagg tggccgaggt cgtggatgag gccgacgagg taggcggccg
     4501 acggatcggg atggcccttg gtctgggcga tttcctgtgc agcggcagcc atggtcaggc
     4561 tgtgtagcca gatctcctcg gccagccccg gcgccagata gcccgccagc ttctgtccaa
     4621 acagcatggc cgttgccagt tgcttggtcc gcgccagacc aacgcggcgg atggcctgca
     4681 gtggctcgag cacctcggcg ccgccgcggc ggtagacgac cgaattggcg acggcgacga
     4741 tgcggatcga caactcgggt tcggaacggc aaatccgcag caggcgttgc tcggcgtcgt
     4801 cggcttctat cgagagcgag aacagctcct gagcgaaggg cgagattggg gggagtttgc
     4861 tggcaccgaa cagtgacata tcggataacc tcgttggctg ggtcgtgatg agttaagaac
     4921 gcccctgcag gggggatcgc gagatgacgg cccggagttt agcacccacg attggtcgat
     4981 tcaggggcgt aggctcggga attggggcgc tgtccccggc ggcgaccaaa gcgattgcat
     5041 ccgtgacgga acggccagtg ttcagccttc attcccttgt tgcgcctagg ctgctgcgtt
     5101 gcgcgccgga ttgcggggat ggcaacactc gggagagcat ttgtggacga aggcgcatca
     5161 ggtcagtggc ttggcatcgg ctggcgcagt cggcacgcga tgcaccgcga tttcctcgcg
     5221 actgatcgct ccacgtcaat tgggccagac gcgtttacgg gaatagccgg aatggccgag
     5281 cgcgcgtacg acgtgccgcg gacgaatttt gttggctgca tgcgaggcgg cgcagctctg
     5341 acgcctcagg ttggtcgctt ctacccgcct gctttcttgc agggattgca gacccggcgg
     5401 ccatttcgtg tgctggcggt ctcgccggac acgctgcgaa tcgacccgag cccggcgctt
     5461 gccgggcggg cgttggaagc gttgtccgtg ccgattatcc cgggtgtcgc cgtcaggaaa
     5521 tgggcggctg ccagcgatcc gctggcggtt ctgaagcggg ctggcgtcgg ctttcagggg
     5581 gcggcgaacg aaactgaaac cgagttcggg ttggccatgc gcctcctgcg tgccgacgac
     5641 ggatttgacg caagtttcta ctcagcgcca cgactggtcc accaccttga tgcggcggcc
     5701 cgcctgctgc tggccgactg caatgcgcgc ctcttgggtg gcttgccgga cggtgcgccg
     5761 gtgctcgacc tgatgagtag ttggactacg cacctgccgg ccgagtccgg gctgacgctg
     5821 accggactgg ggatgaacgc ggccgaactg gcggccaatc cgttgctgac ggagcgtgtt
     5881 ctccacgacc tgaacgagca gccttgcttg ccgttcccta atcggcattt ctcagcggcg
     5941 atttgctctt tgtcggtcga gtatttggta gagccgctgg cagtgttcgc tgagcttgcc
     6001 cgcgtgctgc ggccgggtgc gccagtggta ctgtcgtgga ccgagcgctg gtttccgccc
     6061 aaggccgtat cgatctggtc agagttgcat ccgtacgaac gtgtggcgat ggtggtcgat
     6121 tgcctgcggt tggcactcgg ttggcacgat atcgtcaccg agtcgtaccg aggtttgatg
     6181 cgtccgaccg acgaccgcta cgccgaccaa ctgccggagg ccgacccgct ttatctggtg
     6241 cgggcgttgt cggtgagcag gtgaggcgcg ttcacgtaac cagccggcgg tagatgtcgg
     6301 cgatcttcag cggcagggtc ttcacgtcgt cgaggaccgt gtaacgcgcc ggtccgtaga
     6361 ggtggcgcag gtagtcggcg ccgttgcggt ctatcgtcac gcagtagctg cgaacgccct
     6421 gcgcgcgcgc ttcaagcagg gcgcgtcggg tgtcctcgat gccgtagcgg ctgcggtatt
     6481 cgtcggagaa gtcgtcgggt ttgccatcgg agagcgtgat cagcaggtgg tgtcgcgtcg
     6541 gctggcggat gagatcgcgc gtcagatggc ggatcgccac gcccatccgc gtgtagtcct
     6601 tgggttcgat tgcggcgatg cgccgctgca ccgactcgtc gtaggcctcg gcaaaggcct
     6661 ttacgcgaaa gatgtcgcag cgcgtccgcg tccagccgga gaagccgtag atcgcccagg
     6721 agtcgccaag tgcctcgacc gcctcgccga gcatcaccag cgcctcgcgc tcggcatcgt
     6781 tgacccagcc cttggtcgag ccgctcatgt cgatcatcag catgactgcg agcgagcggt
     6841 cgttacgcgt gcgctggcta tagaggttgg ccgatgcggg agtgccggcg cggcggtcgg
     6901 cccgcgcggc gatcaacgca tcaaggtcga tttcctcgcc gtcaggctgc tggcgcagca
     6961 cgcgattctc gccacgcagc atttcgaagc gccgccgtag ctgcgcgatc tgtgcacggt
     7021 agcggtggcg gacctgcggc acatagtcag cgtcgccttc gcgcaccgtg tgctcgaata
     7081 ggtgacacca gccgctacgg taggcgttac gccggtagtc ccactcgtcg taggtgtagt
     7141 cggcggggcg tgccccgtag tcgaagctgg cgcccggttc ggcaatcgtc gtcggtgccc
     7201 agggcgcgtc gcccgccggc gagagcagtt ccggcggaat ttcgccgaga tcctgcatca
     7261 gcgaacgagc ggcggcggcg gcctgcgggg gcagggcggc gatttcgccg gcggcgaaca
     7321 aggcgaaagg tagcggctcg gccgattgat cggttccggc cggcggcctg atggcgcccg
     7381 gtcttccggc gtgggcagcg agcgcgcggc gcaggatttc cgtttcgcgc gcgatacgtt
     7441 cggcacgcac gcgcagcgcc gctgccagat cgatcgcgcc ggcatgcggc agttcgggtg
     7501 gcgtcggctc gccgtcgtcg cgcagcgcgt ctagcgcgtg cagtgtcccg gcaagcgagg
     7561 ccccggcggc gagtggcgcg atgcgttgcg ccacggtgtc cggccacggc ccgcggaggg
     7621 cggcgatttc ggccgcgagc ccgggcagtt cctcagcgat cgctgcctcg atgcgggcag
     7681 cctcgaggac cgcgaaccag cgccgggcgg cgtcgccatc gtgccagcgc gcgagcgtgg
     7741 cgtcggcatc gccgttgaat gtgccaaaac gggtctggcc ccagaggatg gcggcgcgca
     7801 cacggtaatg cgcgatgttg tcggcatgct tgtaggcatc ggcgatcgcg ctcggcagat
     7861 agaccacctc ggtgtcggtc caggcgcgcc catcgccggc ctcttcgatc cgtaggcggc
     7921 gacccgacaa cccgtgcagc agccgttcga gccgcgattc gaccaattcg aggcgagcca
     7981 cggcgttgcc gcgttcgcgg cggaggcgga agccctcgat gtcgcgcagc atctccatcg
     8041 cggcgcggcc gccggactgg tcatagcgtt cgagcgcggc gaggataagc gcctcgctgc
     8101 cgtcggtatc gaggcgcgcc agcagctggg gcgccagcat cgccaccaga tagccgagtt
     8161 cggtgttggt ttgcgccgcg atctcggccc agcgcaa
//
LOCUS       DATDAA010000290        11967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_91258,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000290 DATDAA010000000
VERSION     DATDAA010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11967
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91258"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /gene="thiL"
                     /locus_tag="VIS73_09025"
     CDS             complement(<1..564)
                     /gene="thiL"
                     /locus_tag="VIS73_09025"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY5763338.1"
                     /translation="MASEFSLIAWHFTRPTPNAVLGPGDDCALLAPSPGMELAVTTDM
                     LVAGTHFLPDADPEALGWKTVAVNLSDLAAMGAQPRWLLLAGALPEADGDWLAAFSRG
                     LFDCADAFGAELVGGDTTRGPLCLSLTAIGELPSGAALRRDGAQVGDELWVSGSPGLA
                     ALGLRHLLDGLTLPETVLAPAMSALHRP"
     gene            complement(575..1060)
                     /gene="nusB"
                     /locus_tag="VIS73_09030"
     CDS             complement(575..1060)
                     /gene="nusB"
                     /locus_tag="VIS73_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY5763339.1"
                     /translation="MSEDRPQREPKPANRRPPGRSARRRARELVVQGLYQWRVAGQDA
                     AAIEHHLSADPEYARADRALFVELLNGALDDSAALIATLTPLLDRPYTDLSPVEAGIL
                     LLAAHELAAHPETPYRVIINEAIELAKTFGGTDGHKYVNGVLDRLAAELRAAEIRGAT
                     R"
     gene            complement(1057..1560)
                     /gene="ribH"
                     /locus_tag="VIS73_09035"
     CDS             complement(1057..1560)
                     /gene="ribH"
                     /locus_tag="VIS73_09035"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY5763340.1"
                     /translation="MAPSTPRFDDIPEVPADLDGSGLSIGIVMARFNIDIGDGLLSAC
                     TAELRKLGVAAEDILITTVPGALEIPLTLQALAESGRFDALVALGAVVRGETYHFEVV
                     SNDSCRGVLEVQLATSVPIANGILTTEDDDQALARMHEKGTDCARTAVEMANLLISLE
                     AEEEDAA"
     gene            complement(1570..2685)
                     /gene="ribBA"
                     /locus_tag="VIS73_09040"
     CDS             complement(1570..2685)
                     /gene="ribBA"
                     /locus_tag="VIS73_09040"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEY5763341.1"
                     /translation="MAPAAFIAPTAEIVAELAAGRMVILVDAEDRENEGDLVIAAEHC
                     TPEAINFMAKHGRGLICLTLTEAHCQQLQLPLMVRDNRTPHGTAFTMSIEAAQGVTTG
                     ISAYDRSHTVRVAAARDARPQDIVQPGHIFPLMAQNGGVLVRAGHTEAGCDLSAMAGL
                     SPASVICEILKDDGTMARLDDLVEFAQTHGLKIGTIADLIQYRSRNESLVERISEREI
                     TTAGGTFTLIAFSDRVADEVHLALVKPGFDAQREALVRVHEPLSVLDFLLAGTGHHAF
                     SIEETLAAIDRFGCGALVLMHRGEGGAELRRRLAPEAATAPSARKWDPRLYGIGAQIL
                     RDIGVRRMRLLTSRTQMPSMAGFDLEITGFVADPSQL"
     gene            complement(2690..3295)
                     /locus_tag="VIS73_09045"
     CDS             complement(2690..3295)
                     /locus_tag="VIS73_09045"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY5763342.1"
                     /translation="MFSGIIASVGTIVDVTPKSGGVRLAIDAGGLDLNDVALGDSIAC
                     NGVCLTVISRASGRFVVDVSSESLACTVGLSAPGPLNLEKALRLADRLGGHLVSGHVD
                     GVGEVLRFDLLGEGADANRLLEIRAPAELARYIARKGSITVNGVSLTTNSVAGDRFTI
                     NLIPHTLGATTLGTLAAGQRVNLEVDLIARYCERLLAAQEG"
     gene            complement(3377..5167)
                     /gene="dsbD"
                     /locus_tag="VIS73_09050"
     CDS             complement(3377..5167)
                     /gene="dsbD"
                     /locus_tag="VIS73_09050"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEY5763343.1"
                     /translation="MLHRLFGLFALLLTFSGPLAAQEFLNPLVAFQPSVRALDDHTLE
                     VRYQIADGYYLYRDRFAFSVAGEGTIQLDAPQFAKGKMKQDELFGLVEVYFKEAVARV
                     TVQRAGSDGALPLSLSLVSQGCADGGICYPPQTQQVSVDLPALGSTTAAAPRGGDESG
                     EIAALLSNASFWLVLVSFFGFGLLLSFTPCVLPMVPILSGIIVGQSGKGHPTPRRRAF
                     MLSLVYVLGMAVTYAAAGIAAGMSGTLLAAALQNVWVLGSFALVFVVLSLSMFGFYEL
                     QLPAFLQSKLSDEANHFKGGTYYGVASMGAVSALIVGPCVAAPLAGALLYISQSGDAL
                     LGGAALFAMALGMGVPLLLVGLSAGSLVPHTGPWMEAVKRVFGVLLLATALWMVSPVI
                     PAWLVMAGWAALLIVSAIFMHALDGLPPHSSGWQRFWKGVGVMMLLYGTALLVGTLAG
                     NRDPLQPLGGMLVRGPSAVAGQAHETEFRRVASVAELDGVVKAAGKPVMLDFYADWCV
                     SCKEMERFTFSDPAVRARLAKFVLLQADVTANSAEDQALLQRFGLFGPPGIIFFTAGG
                     NEVQGLRVVGFQNAETFAGMLDRALASSAI"
     gene            complement(5170..5508)
                     /gene="cutA"
                     /locus_tag="VIS73_09055"
     CDS             complement(5170..5508)
                     /gene="cutA"
                     /locus_tag="VIS73_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEY5763344.1"
                     /translation="MSALLVLTNLPDRDSATAMATALVERGLAACVNSLGACRSTYRW
                     DGRIESAEEFPLLIKTTTERYPALEAAVSELHPYDVPELVAVEITTGLPAYLGWLAEC
                     CKPASDQETT"
     gene            5670..5746
                     /locus_tag="VIS73_09060"
     tRNA            5670..5746
                     /locus_tag="VIS73_09060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5704..5706,aa:Arg,seq:ccg)
     gene            5890..6663
                     /locus_tag="VIS73_09065"
     CDS             5890..6663
                     /locus_tag="VIS73_09065"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEY5763345.1"
                     /translation="MSAGWRWISDGATLFRRNPLALWPVVAGYWLTLGLASALPLIGL
                     LIAAILIPPLAVGTMTACRESAAKRPVTLLTLYVAFRPPGRDFRILQRLFALGALHFA
                     LTLLILGLASLFDGGALFRVVTGAQTLVDAAGQPGVQSAANIALLFTAPLTMAFWFAP
                     VLVAWHDLTIAKAVVFSFVACWRNWKPFIAYGLGLMFISLLVVALVGQFALGLGMLQA
                     TGPLLTILFLLVLAPILFATFYISYRDVFTAPPAVDETA"
     gene            6668..7321
                     /gene="nadD"
                     /locus_tag="VIS73_09070"
     CDS             6668..7321
                     /gene="nadD"
                     /locus_tag="VIS73_09070"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY5763346.1"
                     /translation="MSGRAPLGLFGGTFDPIHLGHLRLAEEAQEFLGLETVRFVPAGQ
                     PPHRAGPGVAAAERLAMVCLATRDNPAFAVDPSEVESDEPSYTVPTLERLRREFGSAR
                     PIVLLLGADAFSALPTWHRWHDLLELAHIAVVHRGGKAPVAAELPAELVQACITRHAE
                     DPAALRESPAGCVLAVPMTPLAISATDIRARLAARRSVRYLLPPEVLDYIRSRRLYA"
     gene            7327..7680
                     /gene="rsfS"
                     /locus_tag="VIS73_09075"
     CDS             7327..7680
                     /gene="rsfS"
                     /locus_tag="VIS73_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY5763347.1"
                     /translation="MDLKKLLKLITDALEDVKARDIEVIPTAKLTAMFDYIVVASGDS
                     NRQVRALAKSVQDKVREAGGEVLSVEGEGTGDWVLVDLGAVVVHVMQPAVRSYYALEE
                     LWSARPVKRKAAGAS"
     gene            7697..8167
                     /gene="rlmH"
                     /locus_tag="VIS73_09080"
     CDS             7697..8167
                     /gene="rlmH"
                     /locus_tag="VIS73_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY5763348.1"
                     /translation="MKLTIVAVGNRQPAWVDDGCQEYLKRMPRELATRVLEVKPEPRS
                     GGRQRAQLLAAERERIEAAVAPRSRRVAMDERGADLTTVALAERLRRWAEDGRDVDLL
                     IGGADGLDAALRDTADETIRLSSMTLPHGLARLLLCEQLYRAHTVLAGHPYHRE"
     gene            8164..8784
                     /locus_tag="VIS73_09085"
     CDS             8164..8784
                     /locus_tag="VIS73_09085"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY5763349.1"
                     /translation="MIATNQARIYLASRSPRRRELLHQIGVPFDTLLFRSGRRDDDDV
                     DETPLANEDPVDYVQRLARAKAAFGVELVRERRLIAQPLLAADTTVVFGDEILGKPGS
                     IDEAAAMLGKLSGGTHRVLTAVAVAYGERIEHALSVSEVRFRPLADEEIQRYARSGEP
                     MDKAGAYGIQGRAATFVSHLSGSYTGVMGLPLFETAALLGKFGIAI"
     gene            8862..10316
                     /gene="rng"
                     /locus_tag="VIS73_09090"
     CDS             8862..10316
                     /gene="rng"
                     /locus_tag="VIS73_09090"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEY5763350.1"
                     /translation="MSEQILINFTPAETRVAVMDQGVVQEVHIERTASRGIVGNVYLG
                     KVVRILPGMQSAFIDAGLNRTAFLHVADILQARENGGSPKPIERLLGEGQSLVVQAIK
                     DPIGTKGARLSTQISLAGRMLVYLPQEKHIGVSQRIEGESERESLRARLTSLVPPDEA
                     GGFIVRTSAETASDDDLRADITYLRKVWQDISAAALTSRAPALLYHELMLGQRVLRDF
                     VTPETEKIVADSRENFQKLAAFAQEYTPAVAPLLDHYTGERPLFDLHGVEDEIQKALA
                     RRVELKSGGYLILDQTEAMTTIDVNTGAFVGHRNFDETIFKTNLEAAQVIARQLRLRN
                     LGGIIIIDFIDMAEREHQDAVLDEFNRALARDHTKMTVSGFTALGLVEMTRKRTRESL
                     AHVLCELCPICDGRGEIKTARTVCYEILRELMREARQFNAREYRIIGSNAVIDLFLDE
                     ESQSLAMLSDYIGKPISLQAESGYVQENFDIVLL"
     gene            complement(10360..10791)
                     /locus_tag="VIS73_09095"
     CDS             complement(10360..10791)
                     /locus_tag="VIS73_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY5763351.1"
                     /translation="MNDRNGYDIEDLKPGISASMAKTVTEADIILFSGVSTDVNPVHL
                     DEEWASTTMFKGRIAHGMLSAGFISAVLANRLPGPGTIYLGQTLKFKGPVRPGDTVRT
                     VCTVKEAIVEKKRAVIETNCYVGDKLVIEGEATVMCTSRAG"
     gene            10904..11800
                     /locus_tag="VIS73_09100"
     CDS             10904..11800
                     /locus_tag="VIS73_09100"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY5763352.1"
                     /translation="MAIAAPVSASRPEQSSQMVDFARLLRGIDIPSCPEVLAALKAEM
                     QAASPNPVRIGRLISQDVALSAGLLKIANSGLFAPARQVTTMADALALLGIGQAFQWI
                     IKELLQQAIAAGSARRFERFWDSAAYTAAICAQLAGSVSGTTRQAAWDFGLFRDCGIP
                     ILSARFPDYGETLRQANDETRHSFTRVEDLRHGSNHAVVGCLMARTWGLAEAVSEAIL
                     CHHDYQVLERDSGVGSEVQTLVALHVLAEQVVALHLGREAPNEWRKGHQTAAAFLGLS
                     RAGVADLVDEVRSQIDRRRVAG"
BASE COUNT         1967 a         3926 c         4094 g         1980 t
ORIGIN      
        1 cggccggtgc agcgctgaca ttgccggagc gagtaccgtt tcgggcaggg tcagcccgtc
       61 gagcaggtgg cgcagaccaa gggcagcaag ccccggtgaa ccggagaccc agagctcgtc
      121 gccaacttgt gccccgtcgc ggcgcagcgc ggcgcccgac ggcagctcgc cgatcgccgt
      181 gaggctgagg cacagcgggc cgcgcgtcgt gtcgccaccg accagctcgg cgccgaaggc
      241 gtcggcgcag tcgaagaggc cgcgggaaaa cgccgccagc cagtcgccat cggcctccgg
      301 gagcgcgccg gcgagcagca gccagcgggg ctgggcgccc atcgccgcga gatcggacag
      361 attgaccgcg acggtcttcc agcccagcgc ctcgggatcg gcgtcgggca gaaaatgcgt
      421 gccagcgacc agcatgtcgg tggtgacggc gagctccatg cccggcgagg gggccagcag
      481 cgcgcaatcg tcgccggggc cgagaactgc gttgggagtc ggtcgcgtga agtgccaggc
      541 gatcagcgag aactcggagg ccacgccgcg gctttcagcg cgtcgcgccc ctgatctcgg
      601 cggcgcgcag ttcggccgcg agccgatcga ggacgccatt gacgtacttg tgcccgtcgg
      661 tgccgccgaa ggtcttggcc agttcgatcg cctcgttgat gatcacgcgg tagggcgttt
      721 cggggtgcgc ggccagttcg tgggcggcga gcagcaggat gccggcttcg accggcgaca
      781 gatcggtgta gggccggtcg agcagcggcg tcagcgtggc gatcagcgcc gcgctgtcat
      841 cgagcgcgcc gttaagcagc tcgacgaaca gcgcgcggtc ggcgcgggcg tattcggggt
      901 cggccgacag gtggtgctcg atcgccgccg cgtcctgccc ggcgacacgc cattggtaga
      961 ggccctgtac caccagctcg cgggcgcgcc ggcgagccga gcggccggga ggccgtcggt
     1021 ttgccggttt cggctcgcgc tgcgggcggt cctcgctcac gccgcgtcct cttcctcggc
     1081 ctcgagcgaa atcagcaggt tggccatctc caccgcggtg cgggcgcaat cggtgccctt
     1141 ctcgtgcatg cgggcgagcg cctgatcgtc gtcctcggtg gtgaggatgc cgttggcgat
     1201 gggtacgctg gtcgccagtt gcacttcgag cacgccgcgg cagctgtcgt tggagaccac
     1261 ctcgaagtga taggtctcgc cgcggacgac cgcgcccagc gcgaccaagg catcgaagcg
     1321 gccgctttcg gcaagcgcct gcagcgtcag cgggatttcc agggcgccgg gaacggtggt
     1381 gatcaggatg tcctcggcgg caacgccgag cttgcgcagt tcggccgtac acgccgagag
     1441 caggccgtca ccgatgtcga tgttgaagcg ggccatgacg atgccgatcg acaatccgct
     1501 cccgtcgaga tcggcgggca cttcgggaat gtcgtcaaag cggggagtgc tgggggccat
     1561 ggtgggttct cacagttggc tgggatcggc gacgaagccg gtgatctcga ggtcgaatcc
     1621 ggccatcgag ggcatctggg tgcggctggt cagcaggcgc atgcgacgga cgccgatgtc
     1681 gcgcaggatt tgcgccccga tgccgtagag gcgcgggtcc cacttgcgcg cggagggcgc
     1741 ggtggctgcc tcgggggcga gacggcgacg cagttcggcg ccgccctcgc cgcgatgcat
     1801 cagcaccagc gcgccgcagc cgaagcgatc gatcgccgcc agcgtctctt cgatcgagaa
     1861 ggcgtggtgg ccagtaccgg ctagcaggaa atcaaggact gagagcggtt cgtggacgcg
     1921 caccagtgcc tcgcgttggg cgtcgaagcc gggcttgacc agcgccaggt gaacttcgtc
     1981 ggcgacccgg tcggagaagg cgatcagcgt gaaggtgcca ccggcagtgg tgatctcgcg
     2041 ttcggaaatc cgctcgacga gcgactcgtt gcggctgcgg tactgaatca ggtcggcgat
     2101 cgtgccgatc ttcaggccgt gggtttgcgc gaactccacc agatcgtcga gccgcgccat
     2161 cgtcccgtcg tccttgagga tttcgcagat caccgaagcc ggcgacaggc cggccatcgc
     2221 cgacaggtcg cagccggcct cggtgtgccc ggcgcgaacc agcacgccgc cgttctgcgc
     2281 catcagcggg aagatgtggc cgggttggac gatgtcctgc gggcgcgcat cgcgggccgc
     2341 ggcgacgcgc accgtgtgcg agcggtcgta ggccgagatg ccggtggtca cgccttgcgc
     2401 ggcctcgatc gacatcgtga aggcggtgcc gtgcggcgtg cggttgtcgc gcaccatcag
     2461 cggtagttgc agctgttggc aatgcgcctc ggtcagcgtc aggcagatca gaccgcggcc
     2521 gtgcttggcc atgaagttga tcgcctcggg cgtgcagtgc tcggcggcga tgacgagatc
     2581 gccctcgttc tcgcggtcct cggcatcgac gaggatgacc atgcggccgg ccgccagttc
     2641 ggccacgatc tcggcggtgg gggcgatgaa ggctgcaggc gccatggttt tagccttcct
     2701 gggcggcgag caggcgctcg caatagcggg cgatcaggtc gacctcgagg ttgacgcgct
     2761 ggcctgcggc cagcgtgccg agcgtggtcg cgccgagcgt gtgtggaatc aggttgatcg
     2821 tgaagcggtc gccggcgacg ctgttggtcg tcaggctaac gccgttgacg gtaatcgagc
     2881 ccttgcgcgc tatgtagcgg gccagttcgg ccggcgcgcg gatctcgagc aggcgattgg
     2941 cgtcggcgcc ctctcccagc agatcaaagc gcagcacctc gccgacgccg tcaacgtgac
     3001 ccgaaaccag atggccgcca agccggtcgg cgagccgcaa ggccttctcg aggttgagcg
     3061 gacccggtgc cgagaggcca accgtgcagg ccagcgactc gctggaaaca tcgaccacga
     3121 agcggccgga tgccctgctg atgacggtca ggcagacgcc gttgcaggcg atcgagtcgc
     3181 cgagcgcgac atcgttcaga tcgagtccgc cggcatcgat cgcgaggcga acgccgccgc
     3241 tcttcggcgt cacgtcaacg atggttccga cgctggcgat aatcccggaa aacacgctgg
     3301 cagttcgctt tcgaggctac tgggaaaggc cgcgagtcta gcacgcgagg ccctgccacg
     3361 gccttcccca aagggcttag atcgcgctgc tggcgagcgc tcggtcgagc atgccggcga
     3421 aggtctcggc attctggaag ccgaccacgc gtagcccctg cacctcgttg cccccggcag
     3481 tgaagaagat gattccaggt gggccaaaca gcccgaagcg ctgcagcagc gcctgatcct
     3541 cggcgctgtt ggcggtcacg tcggcctgca gcaggacaaa tttcgccagc cgggcgcgta
     3601 ccgccgggtc gctgaaggtg aagcgctcca tctccttgca cgagacgcac cagtcggcat
     3661 agaagtcgag catcaccggc ttgccggcgg ccttcacgac gccgtcgagc tcggcgaccg
     3721 aggcgacgcg gcggaactcg gtttcgtggg cctgtccggc gacggcgctc gggccgcgga
     3781 ccagcatgcc gcccagcggc tgcagcggat cacggttgcc ggccagtgtg ccgaccagca
     3841 gcgccgtgcc gtagagcagc atcatgaccc cgacgccctt ccagaaacgc tgccagcccg
     3901 agctgtgcgg cggcagtccg tcaagcgcgt gcatgaagat cgccgagacg atcagcagcg
     3961 ccgcccagcc cgccatgacc agccaggccg gtatcaccgg cgaaaccatc cacagcgcgg
     4021 tggcgagcag aagcacgccg aagacccgct tcaccgcttc catccagggg ccggtgtgcg
     4081 ggaccagcga gcccgccgag aggccgacca gcagcagcgg cacgcccatg cccagtgcca
     4141 tcgcgaacag cgctgcgccg ccgagcagtg cgtcgcctga ctggctgatg tagagcagcg
     4201 ccccggccag cggcgcggca acacaggggc cgacgatcag cgccgacact gcgcccatcg
     4261 aggcgacacc gtagtaggtg ccgcccttga aatgatttgc ctcgtccgag agcttgctct
     4321 gcaggaaagc cggtagctgc agctcgtaga agccgaacat cgacagcgag agcacgacga
     4381 agactagcgc gaagctgcct agcacccaca cgttctgcag cgccgcggcg agcagcgtgc
     4441 ccgacatccc ggcggcgatg ccggcggcgg cgtaggtcac cgccatcccc agtacgtaga
     4501 ccagcgagag catgaaggcg cggcgccgtg gagtcggatg gcccttgccg ctctggccga
     4561 cgatgatgcc ggaaaggatg ggaaccatcg gcagcacgca gggcgtgaac gagagcagca
     4621 ggccgaaacc gaaaaagctg acgaggacga gccagaagct cgcattgctc agcagcgccg
     4681 caatctcgcc cgactcgtcg ccgccgcgcg gcgccgccgc cgtcgtactg cccagcgccg
     4741 gcagatcgac cgaaacctgc tgcgtctgtg gcggataaca gatgccgccg tcggcgcagc
     4801 cctgcgagac cagcgacagc gacaagggca aggccccgtc gctgccggcg cgctgcacgg
     4861 tgacccgcgc gacggcctcc ttgaagtaca cctcgaccag tccgaacagc tcgtcctgct
     4921 tcatcttgcc tttggcaaac tgcggtgcgt cgagctggat ggtgccctcc ccggccacag
     4981 agaacgcgaa gcgatcgcgg tacaggtagt agccgtcggc gatctggtag cgcacttcca
     5041 gcgtgtggtc gtcgagggcg cggaccgaag gctggaaggc gaccagcgga ttgaggaatt
     5101 cctgcgccgc gagcgggccg gaaaaggtca gcagcagtgc gaagaggcca aacagtcggt
     5161 gcagcatcgt caggtcgttt cctggtcgga cgccggcttg cagcattcgg caagccagcc
     5221 gaggtaggcc ggcagtcccg tcgtaatctc gacggcgacg agctccggga cgtcgtaggg
     5281 gtgaagttcg ctgaccgcgg cttcaagcgc cgggtagcgc tcagtcgtgg tcttgatcag
     5341 caggggaaac tcctctgccg attcgatccg gccgtcccag cgataggtcg agcgacaggc
     5401 ccccagggaa ttgacacagg ccgccagccc gcgttcgacg agcgcagtgg ccatcgccgt
     5461 cgcgctgtcg cggtcgggga gattggtcag tacgagcaaa gcggacacgt cgtggctggt
     5521 cgttcggctt gttggcgaag ccctgttccc gcaggcgggc agcgccgagc ttcggcgtcg
     5581 ggactcgaat cttacgccgt ttctgtttcc cgctggcccg gcgctgtcgt agaatgccgc
     5641 ctgttccggc caactgcggc cggctctgcg cgcccgtagc tcagctggat agagtactgc
     5701 cctccgaagg caggggtcgg acgttcgaat cgtctcgggc gcgccaaatt tccacgtctt
     5761 ccgtttccga gccctttctc gtggcgctac ggccatcggg gctgcggcgc cttggtttag
     5821 tattgggcgc tgatcgactt gtccggtgtt tcccaccttg cgaacctgat gcaggcagaa
     5881 aaattgcgca tgagcgccgg atggcgctgg atctccgacg gcgcgacgct gtttcgccgc
     5941 aatccgctgg cgctctggcc ggtggtcgcc ggctactggc tgacgttggg cctcgcgagc
     6001 gcgctcccgc tgatcggcct gctcatcgcg gcgatcctga ttccgccgct ggcggtaggg
     6061 acgatgacgg cgtgccgcga gtcggcggcg aagcggccgg tgacgctgtt gacgctctac
     6121 gtcgccttcc gtccgccggg gcgtgacttc cgtatcctgc agcggctttt tgcgctcggc
     6181 gcgttgcatt tcgcgctgac attactgatc ctcggcctcg cctcgctgtt cgatggcggc
     6241 gccctgtttc gcgtggttac cggggcgcag accctggtcg atgcggccgg ccagccgggc
     6301 gtgcagtcgg cggccaacat tgccctgctg ttcacggcgc cgctgacgat ggctttctgg
     6361 ttcgccccgg tgctggtcgc ctggcatgat ctgaccatcg ccaaggcagt cgtgttctcc
     6421 ttcgtcgcct gctggcgcaa ctggaagcca ttcatcgcct acgggcttgg cttgatgttc
     6481 atttcgctgc tggtggtcgc tctggtcggc cagttcgcgc tcgggctggg catgcttcag
     6541 gcaaccggcc cgttgctcac catcctcttc ctgctggtgc tggcgccgat cctttttgcc
     6601 accttctata tcagctaccg cgatgtcttc acggcaccgc cggcggtaga cgagaccgcc
     6661 tgagacgatg agcggtcgcg ccccgcttgg gcttttcggc ggcaccttcg atccgattca
     6721 tctgggacat ctgcgccttg ccgaagaagc gcaggagttc ctcgggctgg aaacggttcg
     6781 cttcgttccc gctgggcagc caccgcatcg cgccgggccc ggagttgcgg cggccgagcg
     6841 gctggcgatg gtctgcctcg cgacgcgaga caatccggcc ttcgccgtcg accctagcga
     6901 agtcgagtcc gacgagccga gctatacggt accgacgctc gagcggcttc gccgggaatt
     6961 cggaagcgcc cggcccatcg tgttgttgct cggcgctgac gccttttcgg cgctgccgac
     7021 ctggcatcgc tggcacgacc tgcttgagct tgcacacatt gccgtcgtcc accgcggcgg
     7081 caaggcgccg gtggccgcgg aattgccggc cgagctagtt caggcctgca tcacgcgtca
     7141 cgccgaggac cccgccgcct tgcgcgaatc gcctgccgga tgcgtgctgg cggtgccgat
     7201 gacgccgctg gcgatttcgg cgaccgacat ccgcgcgcgg ctggccgccc gccgcagcgt
     7261 tcgctatttg ctgccgcctg aagttctcga ctatattcgg agccgccggc tttacgcctg
     7321 aacggaatgg atctcaagaa actcctcaag ctgatcaccg atgcgctcga agacgtcaag
     7381 gcgcgcgaca tcgaggtgat cccaactgcc aagctgacgg cgatgttcga ctacatcgtc
     7441 gtcgccagtg gcgattcaaa ccgacaggtt cgcgccctgg ccaagagcgt tcaggacaag
     7501 gtccgcgaag ccggcggcga agtgctgtcg gtcgaaggcg aaggaaccgg tgactgggta
     7561 ctcgtcgatc tcggcgccgt cgtcgtccat gtcatgcagc ctgccgtgcg cagctattac
     7621 gcgctcgagg aattgtggtc ggcccgcccg gtcaagcgga aagcggcagg cgcgtcgtga
     7681 tgatcacagg ctgcccgtga aactgaccat cgtcgccgtt ggcaaccgcc agcccgcctg
     7741 ggtcgacgat ggctgtcagg aatacctcaa gcggatgccg cgcgagctgg cgacccgggt
     7801 actcgaagtg aagccggagc cgcgcagcgg cggccggcag cgtgcccagc tgcttgctgc
     7861 cgagcgcgag cgaatcgagg cggcggtggc accgcgcagc cggcgcgtgg cgatggatga
     7921 gcgtggtgcc gacctaacca cagtggcgct cgccgaacga ctgcgccgct gggccgagga
     7981 cggacgcgac gtcgacctgc tgatcggcgg cgccgacgga ctcgacgcgg cgctgcgcga
     8041 cacggccgac gagacgatcc gcctgtcgag catgaccttg ccgcacgggc tggcgcggct
     8101 gctgctctgc gaacagctct accgcgccca cacggtactg gccgggcatc cctaccaccg
     8161 cgaatgattg ccaccaacca agcgcgcatc tatctcgcct cgcgcagtcc gcgccggcgc
     8221 gagctgctgc accagatcgg cgtgcccttc gatacgctgc tctttcgcag cggtcgccgt
     8281 gacgacgatg acgtcgacga gacgccgctg gccaacgagg atccggtcga ttacgtgcag
     8341 cggctggcgc gggccaaggc ggccttcggc gtcgagctgg tgcgcgagcg gcggctgatc
     8401 gcgcagcccc tgcttgccgc cgataccacc gtcgtcttcg gcgacgagat cctcggcaag
     8461 ccggggtcga tcgacgaggc ggcggcgatg ctcggcaagc tctcgggcgg cacgcaccgg
     8521 gtgttgacgg ccgtcgccgt agcctacggc gagcgcatcg aacacgcgct gtcggtgagt
     8581 gaggttcgat tccgcccgct agcagacgag gaaatccagc gctacgcccg ctcgggcgag
     8641 ccgatggaca aggccggcgc ctacggcatc caggggcggg ccgcgacctt cgtcagccat
     8701 ctttccggca gctataccgg ggtgatgggg ctgccgctgt tcgagacggc ggcgctgctc
     8761 ggcaagttcg gcatcgccat ctgagtctgc cgtctcgcgg cacgtgcggc acaaacgctc
     8821 gcgtagaatc ctcggcaacc cagcaatcac cgataggcgc tatgagcgag cagatcctga
     8881 tcaatttcac gcctgccgag acccgcgttg cggtcatgga ccagggcgtg gtccaggaag
     8941 tccacatcga gcggaccgcc agtcgcggca tcgtcggcaa cgtctatctc ggaaaggtgg
     9001 tgcgcatcct accgggcatg cagtcggcct tcatcgacgc cggcctgaac cgcaccgcat
     9061 ttctccacgt cgccgatatc ctgcaggcgc gcgagaacgg cggatcgccg aagccgatcg
     9121 agcggctgct cggcgaaggg caatcgctgg tggtgcaggc gatcaaggac ccgatcggaa
     9181 ccaagggcgc gcggctgtcg acgcagatct cgctggccgg ccgcatgctc gtctacctgc
     9241 cacaggaaaa gcacatcggt gtttcgcagc ggatcgaggg cgagagcgag cgcgagagcc
     9301 tgcgggcgcg cctgaccagc ttggtcccgc ccgacgaggc gggtggcttc atcgtccgca
     9361 ccagcgccga aaccgcatcc gacgacgacc tgcgcgccga catcacctac ctgcgcaagg
     9421 tctggcagga catcagtgcc gcggcgctaa ccagtcgcgc gccggcgctg ctctaccacg
     9481 agctgatgct ggggcagcgc gtgctgcgtg atttcgtcac ccccgagaca gaaaagatcg
     9541 ttgccgattc gcgggagaac ttccagaagc tcgctgcctt tgcccaagag tacaccccgg
     9601 cggtcgcgcc gctgctcgat cactacacgg gcgagcggcc gctgttcgac ctgcacggcg
     9661 tcgaggacga gatccagaag gcactggcgc gccgcgtcga actgaagtcg ggtggttacc
     9721 tgatactcga tcagaccgaa gcgatgacga cgatcgacgt aaacaccgga gccttcgtcg
     9781 gtcaccgcaa cttcgacgaa acgatcttca agaccaatct cgaggcagcg caggtcatcg
     9841 cacgccagct ccggctgcgc aacctcggcg gtatcatcat catcgacttt atcgacatgg
     9901 cggagcgcga gcatcaggat gctgtgctcg acgagttcaa tcgcgccctg gcacgcgacc
     9961 acacgaagat gacggttagc ggcttcaccg cgctgggcct agtcgagatg acccgcaagc
    10021 gcacccgcga atcgcttgcc catgtgctct gcgagctctg cccgatctgc gacggacgcg
    10081 gcgagatcaa gaccgcgcga acggtctgct acgaaatcct gcgcgaattg atgcgcgagg
    10141 cgcgccagtt caatgcgcgc gaataccgca tcatcggcag caacgcggtg atcgatctct
    10201 tccttgatga ggagtcgcag tcgctggcga tgctttccga ctacatcggc aagccgatct
    10261 cgctgcaggc ggaaagcggc tacgtgcagg agaacttcga catcgtgctg ctctgaagcg
    10321 acccgaggct gacgccgcgg gtctgtcctg cgcctatgct tagccggctc gcgaggtgca
    10381 catgacggtc gcttcgccct cgatcaccag cttgtcgccg acgtagcagt tggtctcgat
    10441 caccgcgcgc ttcttttcga cgatggcctc cttgaccgtg cacaccgttc gcaccgtgtc
    10501 gccgggacgg accggcccct tgaacttaag cgtctgcccg aggtagatcg ttcccggtcc
    10561 gggcaaccgg ttggcgagaa cggccgagat gaagccggcc gagagcatgc cgtgggcgat
    10621 gcgccccttg aacatcgtcg tcgatgccca ttcctcgtcg aggtggacag ggttcacgtc
    10681 ggttgaaaca cccgagaaga gaatgatatc ggcctcggtc accgtcttcg ccatcgatgc
    10741 ggagatgccg ggcttgaggt cctcgatgtc gtaaccgttc ctgtcgttca tgatcgtttc
    10801 gccagagtgg aaagccgccg atgatagcgc gccggcgcgc cggtggatgc cagcaggacg
    10861 cgcgcagagt aagctgacgc ttttctccag tcggtccggt tcgatggcta tcgccgcccc
    10921 cgtttctgcc agtcgccccg agcagtcgtc ccagatggtc gacttcgcgc gcttgctgcg
    10981 gggcatcgat attccgtcct gccccgaggt gctcgcggcg ctgaaggccg aaatgcaggc
    11041 ggcttcgccc aatccggtgc ggatcggccg actcatcagc caggacgtgg cgctttcggc
    11101 gggcttgctg aagatcgcca attctggcct gttcgccccg gccagacagg tgacgacgat
    11161 ggctgacgcg ctggcgctgc tcggcatcgg ccaagctttc cagtggatca tcaaggagtt
    11221 attgcaacag gcgatcgccg ccggcagcgc gcgtcgcttc gagcgattct gggattcggc
    11281 cgcctatacg gcggcgatct gcgcgcaact ggccggttcg gtcagcggca cgacgcggca
    11341 ggcagcctgg gatttcggcc tctttcgcga ttgcgggatt cccatcctca gcgcgcgttt
    11401 ccccgactac ggcgagacgc tgcggcaagc caacgatgaa acgcggcact cattcacccg
    11461 cgtcgaggac ctgcgccacg gcagcaacca cgcggttgtc ggctgcctga tggcgcgcac
    11521 ctggggtttg gctgaagcgg tcagcgaagc gattctttgc catcacgact atcaggtgct
    11581 cgagcgcgac agcggcgttg gcagcgaggt gcagaccctc gttgcattgc acgtcctggc
    11641 cgagcaggtg gtcgcgctgc acctcgggcg cgaagcgccg aacgagtggc gcaagggcca
    11701 ccagacggcg gccgcctttc tcgggctgtc gcgcgccggg gtcgccgatc tggtcgacga
    11761 ggtgcgcagc cagatcgacc gccggcgggt cgccggctag ggagtcctgc tagcgggcgg
    11821 cgatcgcctg ccggtataca tcggccgcaa tggtcggact gtctgccgag tagttgaggt
    11881 ggcggacaat gccgtccttg agaccgtaga tccaggcatg taccgcgagc gactggccgc
    11941 gcagccaggc gtcgcggacg atcgtcg
//
LOCUS       DATDAA010000291         3213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_231444,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000291 DATDAA010000000
VERSION     DATDAA010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231444"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VIS73_09105"
     CDS             complement(<1..732)
                     /locus_tag="VIS73_09105"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5763353.1"
                     /translation="MFTLQILVNGVVLGCLYACIAAGFSLVWGVLNVINLMHGSFIVL
                     GSYLAWGAYNAAGLSPWATLLIAAPLFFVVGYLIQRGVLNRVIAAPVLVTLTLTFGLD
                     LILNNAMLLAFKADYRKLILDPPLGTVALGSVVVPVDRIVAMVFALVLTGALYLILRK
                     SRIGRAIVAVRLDRDAATLMGVNVRSIYAAAFAFGTAMAGCAGVLLALIFPISPLASI
                     PYLGKAFVVCVLGGLGSVPGAVVGGL"
     gene            complement(802..2049)
                     /locus_tag="VIS73_09110"
     CDS             complement(802..2049)
                     /locus_tag="VIS73_09110"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5763354.1"
                     /translation="METRMPSKLPNLKSGHSSIARVAASIALALMPAAFAASSFAQDT
                     VRFGAPLPLTGALAPEALKQQQGYDLWAEIVNKSGGLNVGGKKMKVEIVYTDYQSNTP
                     RAVQAAEQLISQEKVQFLFSPFGSGATKAASTVSEKYGIPTIAATASSSQVYDQGYKY
                     LFGTFTPNDTLTNPLSDIVTKKAPEVKRVAILARNDLFPLAIAQEMEKSAKQRGMSVV
                     FFEKYAINTMDHSSALAQIKAANPQWIFATGYINDLVLIRKQMADQRIEAPVVTMVAG
                     PAYQEFIDAAGAGAENISSAAWWHPAARYAGTDIFGSTENYVKLFREKYKSIPDYAQA
                     SASAAGALFQLAIEKAGSVDPAKVRDQLAKMNANTFFGPVKFGPSGQITSLEPPVFQI
                     QGGKTIVVHPASIRQGDLKLGVK"
     gene            2273..3172
                     /locus_tag="VIS73_09115"
     CDS             2273..3172
                     /locus_tag="VIS73_09115"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5763355.1"
                     /translation="MLQQQALRYFAEVARSGSLRHAAEVFDIAPSAVSRQIAQLEDHL
                     QVALFTRSTRGMTLTAAGIELLEHVNESNLRIDRLRRRLDDLSELRRGTVRLAVVEAA
                     TNSFLPEVLSSFSAEHPGIGFQITVCGTAEVSERVLSNAAEVGLAFGSPSRDDLLLRA
                     RIAQPLQLVCRPRHPLSGSGSLALKQLDGVAAALPDRSFGIRRLIDRAADAARVRLRI
                     ALESDSLQLIKNVVGGSDLVTFMPPMTLERERAAGTLVAIDLQGSGFSKATIDVIRAR
                     GHDLSHAASRFLAWLVQAARRHR"
BASE COUNT          598 a         1066 c          974 g          575 t
ORIGIN      
        1 aagtccgcca accaccgcgc cgggaacgct gcccagcccg cccagcacgc agaccacgaa
       61 cgccttgccc agatacggta tcgacgccag cggggagatc gggaagatca gggcaagaag
      121 cactccggcg catcccgcca tcgccgtacc gaacgcgaat gccgcggcgt agatcgaccg
      181 cacgttcacg cccatgaggg tggcggcatc gcggtccagt ctgaccgcga cgatcgccct
      241 gccgatccgc gacttgcgca ggatgaggta aagggcaccg gtgagcacca acgcgaacac
      301 catggccacg atccggtcga ctggaaccac aaccgagccc agcgcaacgg ttcccagcgg
      361 cgggtcgagg atgagcttgc ggtagtcggc cttgaacgcc agcagcatcg cgttgttcag
      421 gatcaggtcg agcccgaagg tgagcgtaag agtcaccaac accggcgcgg caataacccg
      481 attgagcacg ccgcgctgaa tcaggtagcc gacgacgaag aacagcggtg ctgcgatcag
      541 cagcgtggcc cacgggctca agcccgccgc attgtatgcg ccccaagcca gataggaccc
      601 gagcacgatg aacgacccgt gcatcaggtt gatgacgttc aacacgcccc aaacgaggga
      661 gaagcctgcc gctatgcacg cgtacaggca gcccagcacc actccgttga cgaggatctg
      721 aagcgtgaac atggttgccc gaagcgcccg cggatcccga cccgcgcctg cgcgcccccg
      781 agacgtgcga ttgcagttct actacttcac gccgagcttc aggtctccct ggcggatcga
      841 ggccggatgg accacgatcg tcttgccccc ctggatctgg aacaccggag gctcgagcga
      901 ggtgatctgg ccgctcggcc cgaacttgac cggcccgaag aacgtgttcg cgttcatctt
      961 cgcgagttgg tccctcacct tggccggatc gacagatccc gccttttcga tcgccagctg
     1021 aaacagcgcc cctgccgccg acgccgaggc ctgcgcgtaa tccgggattg acttgtactt
     1081 ctcgcgaaac agcttcacgt agttctcggt cgacccgaag atgtccgtcc cggcatagcg
     1141 cgcagccggg tgccaccagg ctgcgctgct gatgttctcg gcacccgcac ccgccgcgtc
     1201 gatgaattcc tgatacgcgg ggccggccac catcgtcacc accggagcct cgattcgctg
     1261 atctgccatc tgctttctga tcagcacgag gtcgttgatg tagccggtcg cgaagatcca
     1321 ctgtgggttt gcggccttga tctgcgccag cgccgacgaa tgatccatgg tgttgatcgc
     1381 gtatttctcg aagaacacca cgctcatgcc gcgctgcttg gccgacttct ccatctcctg
     1441 cgcgatcgcc agcgggaaca ggtcattgcg cgcgagaata gccacgcgct tgacctcagg
     1501 cgccttcttc gtgacgatgt ccgacagcgg attcgtcaac gtgtcgttgg gcgtgaaagt
     1561 gccgaacagg tacttgtagc cctgatcata gacctgcgac gacgacgcgg tcgcggcgat
     1621 cgttggaatg ccatacttct cggatacggt gctggccgcc ttcgtggcgc cggacccgaa
     1681 cggcgagaac aggaattgca ccttttcttg cgaaatcaac tgctcggctg cctgcacggc
     1741 ccgcggcgtg tttgactggt agtcggtgta tacgatctcc accttcatct tcttgccgcc
     1801 gacgttcaag cctcccgact tgttcacgat ctcggcccac aagtcatatc cctgctgctg
     1861 cttgagtgcc tctggcgcga gcgcgccggt cagcggcagc ggagctccga agcgaaccgt
     1921 atcctgcgcg aacgacgaag cggcgaacgc cgcaggcatc agcgccagag cgatcgacgc
     1981 cgcgactcgc gcgattgaag aatgaccgga cttgaggttc ggtagcttgg aaggcatacg
     2041 cgtctccatc gttaacatgc cgcctctatt gaccattgaa gtcaccgcag tcgatgcggt
     2101 cgacttagac taacatcgac tgaattgcgg gggaattgcc ccctattcga cttccgcgtt
     2161 ctaatttgga gaacacgcgg gtggtcggcc tgatcgaaca accgttgccc ggcccgcgcg
     2221 acagtccggt ttcgagcggt ccgcttggac atcaactcga gtgagttccc gaatgctgca
     2281 gcagcaggcg ttgcgatact tcgccgaagt cgcccgatcg ggctctctgc ggcacgctgc
     2341 ggaggtcttc gacatcgcgc cgagcgcggt cagccgccag atcgcgcaac tcgaggatca
     2401 cctgcaggtc gctctgttca cgcgatccac ccgcggcatg acgctcacgg ccgccggtat
     2461 cgaactgctc gagcacgtga acgagagcaa tctgcgaatc gacaggctcc ggcgccggct
     2521 cgacgatctc agcgagttgc gccgcggcac agtacggctg gcggtcgtcg aggcagccac
     2581 gaacagcttt ctgcccgagg tgctttccag cttttccgcc gagcaccccg gcatcgggtt
     2641 ccagatcacc gtttgcggaa ctgctgaggt ctcagaacgg gttctttcga acgcggcgga
     2701 ggttggtctt gcgttcggat cgccatcgcg cgacgacttg ctcctgaggg cgcggatcgc
     2761 gcagccgctg caactcgtct gcaggccccg ccacccgttg agcggaagcg gttcgctcgc
     2821 gctcaagcaa ctcgacgggg tggcagccgc gcttcccgac cgcagcttcg ggattcgccg
     2881 gctcatcgac cgcgccgcgg acgccgcccg ggtccggctg agaattgcgc tcgagagcga
     2941 ctcgctgcaa ctcatcaaga acgtcgtggg cgggtccgat ctggtgacat tcatgccgcc
     3001 tatgacgctg gagcgcgaac gggctgctgg caccctcgtc gcgatcgacc tgcaaggcag
     3061 tggcttttcc aaagcgacga tcgacgtcat cagagcgcgc ggtcacgacc tctcgcatgc
     3121 ggcgtcccgc tttctcgcct ggctcgtgca agcggcgcgt cggcaccgct gagcgcgcga
     3181 cacgcccacc gcgtgcgttc tgatatcaac aac
//
LOCUS       DATDAA010000292        10428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_476113,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000292 DATDAA010000000
VERSION     DATDAA010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10428
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476113"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VIS73_09120"
     CDS             complement(<1..261)
                     /locus_tag="VIS73_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763356.1"
                     /translation="MDQQLIYRKTTLGDEAIQSRAKLSEHELRVILIMVDGKKSVGEI
                     VSKFGNLQAVEQALYRLEGDGFIAPTDGHISDLELERTHTQIQ"
     gene            339..414
                     /locus_tag="VIS73_09125"
     tRNA            339..414
                     /locus_tag="VIS73_09125"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:372..374,aa:Asn,seq:gtt)
     gene            complement(533..2374)
                     /gene="typA"
                     /locus_tag="VIS73_09130"
     CDS             complement(533..2374)
                     /gene="typA"
                     /locus_tag="VIS73_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY5763357.1"
                     /translation="MSRPLRNIAIIAHVDHGKTTLVDQLLRQSGTFAAHQQMTERVMD
                     SNDIERERGITILSKNTAVDYQGTHINIVDTPGHADFGGEVERVLGMVDGVLLLVDAV
                     EGPMPQTRFVTKKALALGLRPIVVVNKVDRDGASPDNAVNLTFDLFDKLGASDEQLDF
                     PIVYASGLDGWAAMSLEAPRENMVPLFETILAHVPPPEAELDAPLQFQISALDYSSYV
                     GRIGVGRIRRGRVKAGQQVLVLFGTEAEMAEHERTPLKAKVNQVFGFKGLEKVSMPEG
                     LAGDIVQITGIEDLVLGATIADPDKPVALPLLRVDEPTLSMQFMVNTSPLAGTEGKFV
                     TSRQVRERLHKELLTNMALRVHDTADAEIFDVSGRGELHLSILLENMRREGFELAVGR
                     PRVVKKVIDGVECEPYEMLTVDIEENHQGAVMESLGLRRGELQDMVPDGRGRVRLEYR
                     IPARGLIGFQGDFMNMTRGTGLKSHIFDEYAPVREGQVAERRNGVLVSQEDGEAVAYA
                     LWKLQERGRMFVSPGDKLYEGMVIGIHARDNDLVVNPVKGKQLTNVRASGKDEAVVLT
                     PPIDLTLESAIEFIADDELVEITPKTVRVRKRFLKEHERKRASREAV"
     gene            complement(2542..3261)
                     /locus_tag="VIS73_09135"
     CDS             complement(2542..3261)
                     /locus_tag="VIS73_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763358.1"
                     /translation="MKTFWKALAVIVLAHLSPVALAADAAELDVQAGEMERLSEIDST
                     EAELAVAERFVTLAGSAENAERLVFALRNGYETTLAGRDGDDQSATRIYPVTGHLGLG
                     NVFVSLALAQEALHQAGIDQASPAEIDAALNGGEVFVDGEAVRLRGVLAQRVDGVGWG
                     RIAKSMGVRLGPVVASIRSANDGLQRGHLQVARSEAGSERSPARVEKADRPERPARPD
                     RPDRPDRPDRPDRPDRPGKAK"
     gene            complement(3330..3722)
                     /locus_tag="VIS73_09140"
     CDS             complement(3330..3722)
                     /locus_tag="VIS73_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEY5763359.1"
                     /translation="MSHTGIDNTHFQQIGGEPAVRELVERFYDLMELETPYTGIRALH
                     PADLAHSRDKLFWFLCGWLGGPQHYIERFGHPRLRARHMPFAIGEPERDQWMACMRQA
                     MGELKLDPRLQRELGDAFHKTADFMRNS"
     gene            complement(3719..5935)
                     /locus_tag="VIS73_09145"
     CDS             complement(3719..5935)
                     /locus_tag="VIS73_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HEY5763360.1"
                     /translation="MSTAKAITSYRPYWAKRFGIAPFLPTSSAEMAALGWDSCDVVLV
                     TGDAYIDHPSFGMALVGRLLEAQGFRVGIIAQPDWRSAEAFRALGKPNLFYGVTAGNM
                     DSMVNRYTSERKVRSDDAYTPGGAPDRRPDRAVTAYAQRCREAFHGVPVVIGSIEASL
                     RRIAHYDYWSDTVRRSVLVDSKADLLLFGNAERALVELAHRLAKGVPIGEIRDLRGSA
                     FMVPIGWMPADDWHALDSTSLDTPGPVAPHPDPYAMEAPGPGGSPTAPAATAQPIRLM
                     SRAERRAALQAERAKTVIRLPAYEAVKDNPMLYAHASRVFHLESNPGNARCLVQSHGS
                     GAGQRDVWLNPPPIPLTTAEMDHVYGLPYARAPHPGYAGAKIPAWEMIRFSINIMRGC
                     FGGCTFCSITEHEGRIIQSRSEQSILNEIEEIRDKTPGFTGVISDLGGPTANMYRMAC
                     KDPEIESSCRLLSCVYPDICPNLGTDHAPLVSLYRKARALPGIKKVLIGSGLRYDLAV
                     RSPEYVRELVTHHVGGYLKIAPEHIAEGPLAHMMKPGIGAYDRFKAMFEKFSKEAGKE
                     QYLIPYFIAAHPGTTDEDMVELALWLKRNGFRLDQVQTFLPTPMALATTMYHTGRNPL
                     KKVAASATGVDVIREGRRRRLHKAFLRWHDPDNWPLLRDALQRMGRSELIGNGKRHLI
                     PSFQPAGTGRPLAAAGADAARLGAGSRGRAPAARSPGERRGPRAAASRPLPAKRGR"
     gene            complement(5969..8491)
                     /locus_tag="VIS73_09150"
     CDS             complement(5969..8491)
                     /locus_tag="VIS73_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5763361.1"
                     /translation="MRFGSTDGGIGQPWKMAARALWRERHLGSLRLLVAATVLAVAAL
                     SSVGFFADRVRQALNAQSHLLLGADLRLVSQQPWDDSTRRTIVDAGADLAETVMFPSM
                     VGHGEAFQLVDVKAVSDGYPLRGRLRISDDIGQDDRVATGVPAPGTVWIDERLVAALG
                     AGPGDVVQLGGASLRIDAILTLEPDRGINFFSLSPRLMLNLADLPQTGLLQTGSRVRY
                     RLLVAAGAARIALLEAALTPRLGRGQQIENAANARPEIRNALDRAERFLGLAAILTVV
                     LSTVAIALAVRRYMQAAYDGLAVMRCLGATQGVIQGLVLRQFLLLALGAALLGIALGF
                     AAHWVLSAFLATLLAIALPLPGWQPAAHGLAVGVLLVMSLILPRLLQLKHLPSLRVIR
                     REIGPPAQSAWFAYGVAGLLVFGLMTLIAGDLRLSIRVFGGFIAAGAFFVGFAYVGVR
                     SLVRWRRVANPSGWLGEIIAAVGRRPLAVAVQVGGLGLGLMALLLLTVTQRELISAWE
                     RAAPADAHNRFVINIQPDQVEAVQALMRDGGIDSPLQPMVRARLVRIGEQPVAPEDFS
                     DERARRLTEREFNLSWRADLPEGNEVAGGRWFATNEGPGAASVEAGLAETLGIKLGDT
                     LQFDLAGTPVSATVVGLRRLKWESMRVNFFVIFSPGTLEDFPASYIASFHAANGQGDV
                     ASRLLQRFPNLTVIDVSAMLEQLRGITDQVIAAVQFLFVFSLFAGVTVLYGTFAVAFD
                     ERRHEIAVMRALGATRARLARTLRLEFAAIGALAGFFAAAGATLIGWVLARQVFEITL
                     APSYWLVPSATVGGSLTIVLVGRYALQALLSAPPMQSLRNAE"
     gene            complement(8497..9864)
                     /gene="radA"
                     /locus_tag="VIS73_09155"
     CDS             complement(8497..9864)
                     /gene="radA"
                     /locus_tag="VIS73_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY5763362.1"
                     /translation="MVKAKTAYVCQGCGASNSKWQGQCAGCGEWNTLVESVVEASRGS
                     GRFQGIAAASSLQVLGDIEARETVRLPTGISEFDRALGGGIVPGAVVLIGGDPGIGKS
                     TLLLQSLSLMAAGHPVLYVTGEESGEQVALRARRLQVDARQVKLLAEINLERILVTLD
                     AERPAVAVIDSIQTVYSEALSSAPGSVAQVREGAAQLTRYAKQSGTAIILVGHVTKEG
                     ALAGPRVLEHIVDTVLYFEGDTHSSFRLVRAVKNRFGAVNELGVFAMTDRGLRGVTNP
                     SALFLTPHEQPVAGACVLVTQEGTRPLLVEVQALVDSGHGGSPRRLSVGLEQNRLAML
                     LAVLHRHAGIFCLDQDVFVNAVGGVRIAEPAADLAILLAIVSSLRNRPLPDKLAVFGE
                     VGLAGEVRPAPRGQERLREAAKLGFRTAIVPKANLPRRGMEGIEGMEVIGVERIEQAV
                     TAALA"
     gene            complement(9864..>10428)
                     /gene="alr"
                     /locus_tag="VIS73_09160"
     CDS             complement(9864..>10428)
                     /gene="alr"
                     /locus_tag="VIS73_09160"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY5763363.1"
                     /translation="RTLARDWTGEISVANSATILRFPEVGGAWSRPGIMLYGGSPFGW
                     SDPARRAEAIGLRPAMTLRSQLLSVQQLARGETVGYGRTFVAEAGMRVGVVACGYADG
                     YPRHAPSGTPVLVNGVRTRLIGRVSMDMLTVDLTPCPAAQAGDPVVLFGEGLPADEVA
                     AAAGTISYELFCALAPRVPVRELARVA"
BASE COUNT         1829 a         3521 c         3360 g         1718 t
ORIGIN      
        1 ctgaatctgg gtatgggtac gttccagttc aaggtccgaa atatggccat cggtcggcgc
       61 aatgaacccg tcgccttcga gtcgatacag cgcctgttcg accgcctgca ggttgccgaa
      121 cttcgaaacg atttcgccga cgctcttctt gccgtcgacc atgatcagga tgacccgaag
      181 ctcgtgctcg gataacttgg cgcgcgactg gatcgcttcg tcgccaagtg tggtttttcg
      241 atagatcagt tgttggtcca cgtcgctgtc gtccttgatc gggtctctgt tttttcttga
      301 cgagactgcg tccggcgcct attatgcgcg ccttcgattc cccgatagct cagttggtag
      361 agcaacggac tgttaatccg tgtgtccctg gttcgagccc aggtcgggga gccagaatac
      421 gaaaaagggt cagcatcgcg ctggcccttt ttctttgctg aatcggcaac gccgcgccga
      481 ttatcacacc gcaatagcgg caaatccacc cggtccgacc gcgcgcggcg gctcagactg
      541 cctctcgcga ggcgcgcttg cgctcatgct ccttgaggaa gcgcttgcgg acacgaaccg
      601 ttttcggagt gatctcgacc agttcgtcat cggcgatgaa ctcgattgcc gactcgagtg
      661 tcaggtcgat tggcggagtc agcaccacag cctcgtcctt gcccgaagca cggacgttgg
      721 tgagctgctt gcccttgacc ggattgacga cgaggtcgtt gtcgcgggcg tgaatgccga
      781 tgaccatgcc ctcgtagagc ttgtcgccgg gcgatacgaa catccggccg cgctcctgca
      841 gcttccacag cgcgtaggct acggcctcgc cgtcttcctg cgacaccaac accccgttgc
      901 ggcgctccgc aacctgccct tctcgaaccg gcgcgtactc gtcgaagatg tgactcttca
      961 gaccggtacc gcgcgtcatg ttcatgaagt cgccctgaaa tccgatcagg ccgcgcgctg
     1021 gaatccggta ctcaaggcga acccggccgc ggccatcggg aaccatgtcc tgcagctcgc
     1081 cacgacgcag ccccagcgat tccatgaccg ccccctggtg attctcctcg atatcgaccg
     1141 tgagcatctc gtagggctcg cattcgacgc catcgatgac tttcttgacg actcgcggac
     1201 gaccgacagc cagctcgaag ccctcacgtc gcatattctc tagaagaatc gacagatgca
     1261 gttcaccacg tccagagacg tcaaagatct cggcgtcggc cgtgtcgtga acgcgcaagg
     1321 ccatgttggt cagcagttcc ttgtgcagcc gttcgcgaac ctgccggctg gttacgaact
     1381 tgccttcggt gccggccagc ggagaggtgt tgaccatgaa ctgcatcgac agcgtcggct
     1441 cgtcgacgcg cagcagcggc agcgcgacgg gcttgtctgg gtcggcgatt gtggcgccaa
     1501 gcacgaggtc ctcgatgccg gtgatctgca cgatgtcgcc tgcaagacct tcgggcatcg
     1561 acaccttctc cagtcccttg aagccgaaaa cctgattgac cttggccttt agtggcgtcc
     1621 gctcgtgctc ggccatttcc gcctcggtgc cgaatagcac cagcacctgc tggccggcct
     1681 tgacccgtcc gcggcgaatc cggccaacgc cgatgcgacc gacgtagctc gaatagtcga
     1741 gcgccgaaat ctggaattgc agcggcgcat cgagttcggc ttccggcggc ggcacgtgcg
     1801 caaggatggt ctcgaacagg ggcaccatgt tctcgcgtgg cgcctccagc gacatcgccg
     1861 cccagccatc gaggcccgag gcgtagacga tcggaaaatc gagctgttcg tcgctcgcgc
     1921 cgagcttgtc gaacaggtca aaggtgaggt tcaccgcatt gtcaggcgag gctccgtcac
     1981 ggtcgacctt gttgaccacg acgatcggcc gcaggccgag cgccagcgcc ttcttggtga
     2041 caaagcgcgt ctgtggcatc ggcccctcga cggcatcgac cagcaacagc accccgtcga
     2101 ccatgcccag cacgcgttcg acctcgccac cgaagtcggc gtgtcccggc gtgtcgacga
     2161 tattgatgtg agtgccctgg tagtccaccg ccgtgttctt ggagaggatg gtgatgccac
     2221 gctcgcgctc gatgtcgttg gaatccatta cgcgctcagt catctgctgg tgggcggcga
     2281 aggttccgga ctggcgcagg agctggtcga cgagggtggt cttgccgtgg tcgacgtggg
     2341 cgatgatggc gatgttgcga agcgggcggc tcatggtgca gtgcaataaa aagggaggcc
     2401 gcatgctacc acatggcagc cctgaatccc gcgccgaatc tttgattttc tttatctttt
     2461 ccgaaagagg agcgaacgcg cgacgatgtc agcgcttggt gcgcgccgcg tttcggcaga
     2521 cgtagatgcg cgaagggcgg cttacttggc cttccccggt cgatcgggcc ggtccggtcg
     2581 atcgggccgg tccggacgat cgggcctgtc agggcgcgcc ggccgctcgg gccgatcggc
     2641 cttttccacc cgcgccggcg atcgctcgct gcctgcctcg ctgcgggcaa cctgcaggtg
     2701 accccgctgc aggccatcgt tggccgagcg aatcgaagcc acgaccggac caagccgaac
     2761 ccccatcgac ttggcgatgc gcccccagcc gacgccgtcg acgcgctgcg ccaatacgcc
     2821 acgcaggcgc acggcctcgc cgtcgacgaa tacttcgccg ccattcaatg cggcgtcgat
     2881 ttcggccgga ctagcttggt cgattccagc ctgatgcaag gcctcctgcg ccagcgccag
     2941 cgatacgaag acgttgccga gtcctaggtg gccggttacc ggatagatcc gcgttgccga
     3001 ctggtcatcg ccgtcgcgcc ccgccagtgt tgtctcgtag ccattgcgca gggcgaacac
     3061 caatcgctcg gcgttctcag cggacccggc cagcgttacg aagcgctcgg ctacggccag
     3121 ttcggcctcg gtgctgtcga tttcggacaa gcgctccatt tccccggcct gaacatcaag
     3181 ttcggctgcg tcggcggcca gcgccactgg cgacaaatgc gccagaacga tgactgcgag
     3241 ggctttccag aatgtcttca ttgctttccc catctcggaa tgcgtgaatg cgtaccgatc
     3301 cttcttacag gcagtatagt ccacgccacc tagctatttc gcatgaaatc cgcggtcttg
     3361 tggaatgcgt caccgagttc tcgctgcagg cgcggatcga gcttgagttc gcccatcgcc
     3421 tgccgcatgc aggccatcca ctggtcgcgc tcgggctcgc cgatggcaaa aggcatgtgc
     3481 cgcgcccgca gccgcggatg cccgaagcgc tcgatgtagt gctgcggccc acccagccag
     3541 ccgcacagga accagaacag cttatcgcgg gaatgggcga ggtcggccgg gtgcagggcg
     3601 cgaattcccg tgtagggggt ctccagctcc atcaggtcgt agaaacgttc gaccagctcg
     3661 cggaccgcgg gctcgccgcc gatctgctgg aagtgcgtgt tgtcgatgcc agtgtggctc
     3721 atcgtccgcg cttcgccggc agcggccgcg acgcggcggc acgcggaccg cggcgctcgc
     3781 ccggactccg cgccgccggc gcccgcccgc gacttccggc gccgaggcgc gctgcgtccg
     3841 ccccggccgc agctagcggc cggcctgtgc cggctggctg gaagctcggg atcaggtggc
     3901 gcttgccgtt gccgatcagc tcgctgcgtc ccatccgttg cagggcatcg cgcagcagcg
     3961 gccagttatc gggatcgtgc cagcgcagga aggccttgtg caggcgacga cggcgaccct
     4021 cgcggatgac gtcgacaccg gtcgctgacg ccgcaacctt cttcagcggg ttcctgccgg
     4081 tgtgatacat cgtcgtcgcc agcgccatcg gcgtcggcag gaaggtctgc acctggtcga
     4141 ggcggaagcc gttccttttc agccacagcg ccagctcgac catgtcctcg tcggtcgttc
     4201 cgggatgcgc ggcgatgaaa tacgggatca ggtactgctc cttgccggcc tccttcgaga
     4261 acttttcgaa catcgccttg aagcggtcgt aggcgccgat tccgggcttc atcatgtgcg
     4321 cgagcggccc ctcggcgatg tgctcggggg cgatcttcag gtagccgccg acgtggtgcg
     4381 tcaccagttc cctgacgtac tcgggagagc gcacggcgag gtcgtagcgc aggcccgagc
     4441 cgatcagcac cttcttgatt ccgggcagcg cgcgggcctt gcggtagagc gagaccagcg
     4501 gcgcgtggtc ggtaccgaga ttggggcaga tgtccggata gacgcaggac agcaaccgac
     4561 aggacgactc gatctcggga tccttgcagg ccatgcggta catgttggcg gttggcccgc
     4621 cgaggtcgga gatcaccccg gtgaaacccg gcgtcttgtc gcggatttcc tcgatctcgt
     4681 tgaggatcga ctgctccgag cggctctgga tgatgcgacc ctcgtgctcg gtgatcgagc
     4741 agaaggtaca gccgccaaag cagccgcgca tgatgttgat cgaaaagcgg atcatctccc
     4801 aggctgggat ctttgccccc gcgtaaccgg ggtgcggtgc ccgtgcgtag ggcaggccgt
     4861 agacgtggtc catctccgcg gtggtcagcg gaatcggcgg cggattgagc cagacgtcgc
     4921 gctgccctgc cccgctgcca tgcgactgca ccaggcagcg cgcgttgccg ggattcgatt
     4981 caaggtgaaa gacgcgcgag gcatgggcgt agagcatggg gttgtccttg accgcctcgt
     5041 aggccggcag acggatcacc gtcttcgcgc gctcggcctg cagggccgcc cgacgctcgg
     5101 cgcgactcat cagtcggatc ggctgggccg ttgcggcggg cgccgtcggc gaaccccccg
     5161 gccccggcgc ctccatcgcg tagggatcgg gatgcggcgc aaccgggccg ggtgtatcga
     5221 gcgacgtcga atcgagtgca tgccagtcgt cggccggcat ccaaccgatc gggaccatga
     5281 aggccgagcc gcgcaggtcg cggatctcgc cgatcgggac gcccttcgcc aagcggtgtg
     5341 ccagttcgac gagcgcccgc tcggcattgc cgaagagcag caggtcggcc ttcgaatcga
     5401 ccagcaccga gcggcgcacg gtatcggacc agtagtcgta atgcgcgatg cgccgcaagc
     5461 tggcctcgat cgagccgatc accactggca cgccgtgaaa ggcttcacga cagcgctgcg
     5521 cgtacgcggt cacggcgcgg tcgggacggc ggtcaggcgc gccgcccggg gtgtaagcgt
     5581 cgtccgagcg caccttgcgc tccgaggtat agcgattgac catcgaatcc atgttcccgg
     5641 cggtcacgcc gtagaacagg ttcggcttgc ccaaggcgcg gaaggcttcg gccgaacgcc
     5701 aatcgggttg ggcgatgatc ccgacgcgaa agccctgtgc ctcgagcagg cggccgacca
     5761 gcgccatgcc gaagctcggg tgatcgatgt aggcgtcgcc ggtgaccagc acgacgtcac
     5821 aggaatccca gcccagcgcc gccatttcgg cagacgaggt cggcaggaag ggggcgatgc
     5881 cgaaacgctt cgcccagtag gggcggtacg aggtaatggc tttcgcggtg ctcatcggtc
     5941 tcgccgaagg atcgggtttc atgtcatctc actcggcatt gcgcagggac tgcatcggcg
     6001 gcgccgacag cagtgcctgc aaggcatagc gcccgaccag cacgatcgtc agcgagccgc
     6061 cgacggttgc cgacggcacc agccagtagc tgggcgccag cgtgatctcg aagacctgtc
     6121 gcgccagcac ccagccgatc agcgtcgcgc cggcggccgc gaaaaaaccg gccagcgctc
     6181 cgattgccgc gaattcgagc cgcagggtgc gggccagacg ggcacgggtt gccccaagcg
     6241 cgcgcatcac ggcaatctcg tgccgccgct catcgaaggc gacggcgaag gtaccgtaga
     6301 gaactgtaac gccggcaaac agcgagaaga cgaacaggaa ctgcacggca gcgatgacct
     6361 gatcggtaat cccgcgcagt tgttcgagca tggcgctgac atcaatgacg gtcaggttcg
     6421 gaaaacgctg cagcaggcgg ctggcgacat cgccctgccc gttcgcggca tggaagctgg
     6481 cgatatagct ggcggggaag tcttcgagcg tacccggtga gaagatcacg aagaagttga
     6541 cgcgcatcga ttcccatttg agccgccgca ggccgaccac cgtcgccgat accggagttc
     6601 cggccagatc gaactgcaag gtgtcgccaa gcttgatgcc gagcgtctcg gcgaggccgg
     6661 cctcgaccga ggcggcgccc ggaccctcat tggtggcaaa ccagcggcca ccggcaacct
     6721 cgttgccttc gggtagatca gcgcgccagg aaaggttgaa ctcgcgctcg gtcaggcgcc
     6781 gcgcgcgttc gtcggaaaaa tcttcgggcg caacaggctg ctcgccgata cggacgagcc
     6841 gcgcccgcac catgggctga agcggcgagt cgatgccacc gtcacgcatg agcgcctgta
     6901 cagcctcgac ctgatcgggc tggatgttga tgacgaagcg gttgtgcgcg tcggctggcg
     6961 ctgcgcgttc ccaggccgag atcagctcgc gctgggtcac cgtcagcagc agcagcgcca
     7021 tcagccccag ccctaggcca ccaacctgca cggcaactgc cagcggccga cggccgaccg
     7081 cggcaatgat ctcgcccagc caaccggaag gattggcgac ccggcgccag cgcaccagcg
     7141 accggacacc gacataggcg aacccgacga aaaaagcacc cgcagcgatg aagccgccga
     7201 aaacccggat gctcaggcgc aggtcgccgg caatcagcgt catcagacca aagaccagca
     7261 gccccgcgac cccgtaggcg aaccacgccg actgcgccgg gggcccgatc tcgcggcgaa
     7321 tgacgcgcag gctaggcaag tgcttcagtt gcaacaggcg cggcaggatg aggctcatca
     7381 ccagcaagac gccaaccgcc agcccgtgcg cggccggctg ccagcccggc agcggcaggg
     7441 caatagcaag cagcgtcgct aggaaggccg agagcaccca gtgcgccgcg aagccgagcg
     7501 caatgcccag cagcgccgca ccgagtgcga gtagcaggaa ctgccgcagc accagaccct
     7561 ggatcacccc ttgcgtcgcg ccgaggcagc gcatcacggc caagccgtcg taggccgcct
     7621 gcatgtagcg gcgtacggcc agcgcgatcg ctaccgtcga cagcaccacg gtcaggatcg
     7681 ccgcgaggcc gagaaaacgt tcggcgcgat cgagtgcatt gcgaatctcg ggacgggcgt
     7741 tggccgcgtt ctcgatctgc tggccgcggc ccagccgcgg cgtcagtgcc gcctcgagca
     7801 gcgcgatacg ggccgcgccg gcggcaacaa gcaggcgata acgaacccgg ctgccggtct
     7861 gcagcagccc ggtctgtggc agatcggcga gattaagcat cagccgcggc gagaggctga
     7921 agaagttgat gccgcgatca ggttccagcg tcaggatcgc gtcgatccgc aggctcgcgc
     7981 cacccagttg aacgacgtcc ccgggtccgg cgccgagtgc cgccaccagc cgctcgtcga
     8041 tccagaccgt gcccggtgcc ggaacaccgg tggcaacccg gtcgtcctga ccgatgtcgt
     8101 cgctgatgcg cagccggcca cgcagcggat agccgtcgct gactgccttg acatcaacga
     8161 gctggaaggc ctctccgtgg ccgaccatgc tcggaaacat gacggtctcg gccagatcgg
     8221 caccggcatc gacgatcgtc cgccgcgtcg aatcgtccca aggctgctgc gagaccaggc
     8281 gcaggtcggc gccgagaagc aagtgcgact gggcgttaag cgcctgccgc acgcggtcgg
     8341 caaagaagcc gacgctgctc agcgcggcga cggcaagcac ggtcgccgcg accagcaggc
     8401 gcagcgagcc gagatggcgt tcgcgccaga gcgcgcgcgc cgccattttc cacggctggc
     8461 cgatgccgcc gtcggtacta ccgaagcgca tgcccgtcac gccaatgccg ccgtgaccgc
     8521 ctgctcgatc cgttccactc cgatcacttc cattccctcg attccttcca tgccgcgccg
     8581 gggcagattg gccttgggca cgatcgccgt acggaagccc agcttcgccg cctcgcgcag
     8641 gcgctcctgc ccgcgcggcg ccggacgcac ctcgcccgcc agaccgactt cgccgaagac
     8701 cgccagcttg tcgggcagcg gccggttgcg cagcgaggag acgatcgcca gcaggattgc
     8761 caggtcagcc gccggctcgg cgatccgcac gccaccgact gcattgacga agacatcctg
     8821 atcgaggcaa aaaatcccgg cgtgtcgatg taatactgca agcagcatcg ccagccggtt
     8881 ctgttcgagg ccaaccgaga gccgtcgcgg gctgccgcca tggccagaat cgaccagcgc
     8941 ctgtacttca actagcaatg gccgcgtacc ttcctgagtc accaggacgc aggcgccggc
     9001 gaccggttgc tcgtgcggcg tgaggaacag cgccgacggg ttggtgacgc cgcgcagccc
     9061 gcggtcggtc atcgcgaaca cgccgagttc gttcaccgcg ccgaagcggt tcttgactgc
     9121 acgcacgagc cggaacgacg aatgcgtatc gccttcgaaa tagagcacgg tatcgactat
     9181 atgctcgaga acgcgcgggc cggccagcgc tccctccttg gtcacgtggc caacgaggat
     9241 gatcgccgtg ccgctctgct tggcgtagcg cgtcagctgc gcggccccct cgcgcacctg
     9301 cgctaccgat cccggcgccg agctcagcgc ctccgaatag acggtctgga tcgaatcgat
     9361 caccgcgacc gccggccgct cggcgtcgag cgtcacgaga atgcgttcga ggttgatctc
     9421 ggcgagcagc ttgacctgcc gcgcatcgac ctgcagccgc cgcgcgcgca gcgcgacctg
     9481 ctcaccggac tcctcgcccg tgacgtagag caccgggtgg ccagccgcca tcagcgacaa
     9541 cgattgcagc agcagcgtgc ttttgccgat tcccggatca ccaccgatca gcaccaccgc
     9601 gccgggcacg atgccgccac ccagggcccg gtcgaattcc gagataccgg tcggcaggcg
     9661 caccgtttca cgcgcttcga tgtcgccgag cacctgcaag ctgctcgccg cggcgattcc
     9721 ctggaaacgc ccgctgcccc ggctggcctc gacgacggat tcgaccagcg tgttccactc
     9781 tccgcaaccc gcgcactggc cctgccattt ggaattgctg gcgccgcagc cctggcagac
     9841 gtaggccgtc ttcgctttca ccatcacgcg acccgcgcca gttcgcgaac cggaacccgc
     9901 ggtgccagcg cgcagaacaa ctcgtagctg attgtccccg ccgctgccgc cacctcgtcg
     9961 gccggcaggc cctcgccgaa cagcacgact ggatcgccgg cctgtgctgc aggacaaggc
    10021 gtcagatcga cggtcagcat gtccatcgat acgcggccga tgagccgcgt ccgtacaccg
    10081 ttgaccagca ccggcgtccc ggagggcgcg tgccgcgggt acccgtcggc gtagccgcag
    10141 gcaaccacgc caacgcgcat cccggcctcg gcaacgaaag ttcgcccgta gccaaccgtc
    10201 tcgccacgcg ccagttgctg gaccgacaac aactggctgc gcagggtcat tgccggccgt
    10261 agcccgattg cttcggcgcg gcgcgccgga tcgctccagc cgaaggggct gccgccgtag
    10321 agcatgatcc ccggccggct ccaggcgccg ccgacctcgg gaaaacgcag aatcgtcgcc
    10381 gagttggcga cgctgatctc gccggtccag tcgcgcgcca gcgtccgg
//
LOCUS       DATDAA010000293         2048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_371720,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000293 DATDAA010000000
VERSION     DATDAA010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371720"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VIS73_09165"
     CDS             <1..677
                     /locus_tag="VIS73_09165"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5763364.1"
                     /translation="GGTAIIFMAHRSARRDQEDMFMHRFSTLVVAAFLSLCVTVAWSG
                     DVKKGWAAYNALDYAAAKAEWQELADAGDAEAAYGIGMLYGNGFGVDMDDALALKYYG
                     IAAEKGHKEAQFNLAVMHQNGWGMSPNEKLANEWYLKAADQGQKQAQMALGRYYAMDF
                     SSSYDPVKAYKWFSLAKRLGDIDASTKQDFIMSRMTAEQIAEGDALVEAWASTNKEIV
                     ANAPEF"
     gene            complement(745..>2048)
                     /locus_tag="VIS73_09170"
     CDS             complement(745..>2048)
                     /locus_tag="VIS73_09170"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY5763365.1"
                     /translation="EHGSLPWSELLAEPARIAREGYTLLPGAAARHQTGFAQFSDNPG
                     FQSVFVEDDGTAFDAGDVIVQPALARTIERLAAHGADDFYHGQIAQQIAADMAANGGH
                     VTTEDLAAYEVLDGRYITTRYRNHQIHTLAAPAGGGLVVKTLNILENFDLAEFSEVQW
                     AAVVNQALAIAINSMADDRAELDLEKVTSKAWAAAQAATITVPAAATESTASAPLTPQ
                     VASSRTDWSGENWGADSHHTTHFTIADCDGATVSITQTVGPLFGSSVITPDLGFVYAT
                     TMGSYLSEADQAPGSRPRTTIAPTFVTLDGDVVLVLGAAGGIHILSAIVQTISRHLDQ
                     GHDPVSAVALPRVHPERLTTESGERRTYGDKVNLEMTAERGWAPEIAGQLEDAGFEVL
                     PVEEHASFGRVHLIADSGGSWLGVADLDWEGTSAGTSCTNR"
BASE COUNT          363 a          616 c          681 g          388 t
ORIGIN      
        1 cgggaggcac tgcgatcata ttcatggcgc acaggagtgc gcgccgcgat caggaggata
       61 tgttcatgca caggttctca acgctcgtcg tcgctgcgtt cctgtccctg tgcgtgaccg
      121 tcgcctggtc tggcgacgtc aaaaaaggct gggctgccta taacgcgctc gactacgcag
      181 ccgcgaaagc cgaatggcag gaactggcgg atgccggcga tgccgaggcg gcctatggca
      241 tcggcatgct gtacggcaac ggcttcggcg tcgatatgga tgacgcgctg gcgctgaagt
      301 actacgggat tgcggccgaa aaaggccata aagaagcgca gttcaacctg gccgtgatgc
      361 accagaatgg ctggggcatg tcgcccaacg agaaacttgc gaacgagtgg tatctgaaag
      421 cagccgacca gggccagaag caggcccaaa tggcgctggg ccgctattat gcgatggact
      481 tctccagctc atatgacccg gtgaaggctt acaagtggtt tagcctggcg aaacggctcg
      541 gtgacattga tgcttccacc aaacaggatt tcattatgag ccgcatgact gccgaacaaa
      601 tcgccgaggg tgatgccctg gtcgaggcat gggcaagcac gaacaaggaa atagtcgcta
      661 acgcccccga attctagatc ttctctgctc tctctccgag ggtgccgggc ccggccagcg
      721 atggtcgggc ccgatttgat tctgctaacg attggtgcaa ctcgtaccgg ccgacgtgcc
      781 ttcccagtcg agatcagcga cgccaagcca gctgccgccc gagtcggcga tgagatgcac
      841 gcgaccaaaa gaggcgtgtt cttcgaccgg caacacctcg aaaccggcat cttcgagttg
      901 gccggcgatc tccggcgccc agccgcgttc ggccgtcatc tcgagattga ccttgtcgcc
      961 ataggttctg cgttcgccgg actccgtggt gagtctctcc gggtggacgc ggggcagggc
     1021 gaccgccgag accgggtcgt ggccctggtc gaggtggcgc gagatcgtct gcacgatggc
     1081 cgagaggatg tggatgccgc ccgcggcacc gagtaccagc acgacgtcac cgtcgagcgt
     1141 tacgaatgtc ggtgcaatgg tcgtgcgcgg ccgcgatccc ggcgcctggt cggcttcgga
     1201 cagatagctg cccatggtgg tggcatagac gaagccgagg tcgggcgtga tgacgctcga
     1261 gccgaatagc gggccgaccg tttgcgtaat gctcaccgtc gcaccgtcgc agtcggcgat
     1321 cgtgaaatgc gtcgtgtgat ggctgtcggc gccccagttt tcgccactcc agtccgttct
     1381 cgaagacgct acttgtggtg tcagtggcgc gctcgccgtc gactccgtgg ccgccgccgg
     1441 cacggtaatc gttgccgcct gtgccgccgc ccaggccttg ctcgtcacct tttcgaggtc
     1501 gagttccgcg cggtcgtcgg ccatgctgtt gatggcgatc gcgagcgcct ggttgacgac
     1561 cgccgcccat tgcacctcgc tgaactcagc caggtcgaag ttctcgagga tattgagcgt
     1621 tttgacgacg aggccaccgc ctgccggcgc cgcgagcgtg tggatctggt ggttgcggta
     1681 acgcgtcgtg atgtagcgac cgtcgaggac ctcgtacgcg gcgagatctt ctgtcgtcac
     1741 gtggccgcca ttggccgcca tgtccgcggc gatctgctgc gcgatctgcc cgtggtagaa
     1801 gtcgtcggcg ccgtgtgcgg cgaggcgctc gatcgtacgg gcaagcgcgg gttggacgat
     1861 gacgtcacct gcatcgaatg ccgtgccgtc gtcctcgacg aagacgctct gaaaccccgg
     1921 gttatcgctg aattgcgcga agccagtctg gtgacgcgcg gccgcaccgg gcaacagggt
     1981 gtagccctcg cgtgcgatac gtgccggctc ggccagcagc tcggaccagg gaagggagcc
     2041 gtgttcgg
//
LOCUS       DATDAA010000294         2078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_476367,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000294 DATDAA010000000
VERSION     DATDAA010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476367"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VIS73_09175"
     CDS             complement(<1..604)
                     /locus_tag="VIS73_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY5763366.1"
                     /translation="MYRGLSNQDLMTGALDGLINKLDLKGQTLGEVALGAVVKHSRDW
                     NLARECVIESGLSIRTPAFDLQRACGTSIEAAIQIANKIALGQIDAGIAGGTDTVSDA
                     PIVYPDPYRHMLLDMHAAHSLMDRIKPLFRFRPGFLKPELPGVTEPRTGLSMGESCEL
                     TAKEWDVQREHQDQLALASHMKAAAAYDEGFYEDLVVPFND"
     gene            757..>2078
                     /locus_tag="VIS73_09180"
     CDS             757..>2078
                     /locus_tag="VIS73_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY5763367.1"
                     /translation="MFATSPATRRQVSSIARISGPLIINFLAVAGMNLADVMMSGRLG
                     ADALAAVAVGSSSWMLAFSACLGLLMALSPIISRLYGAGEMHKIGRYARHGMYLGLGL
                     GVVILLVGRSNAQALLTMIGIDPEFRPLAITYVRALLFGAPGILIFIALRFTTEGIGY
                     TRPVMFTSIFSLACNVFLNYTLMFGNFGAPAMGVEGCAYASAITMWLVACALGLYVSV
                     SPKLKPLKVFTRIGQFRPKLFREIIALGVPISITITAEVGLFSVVSVMVGTRGVDITA
                     AHQIALSYASTMFMIPLALSSATTVQVGHMLGAGRDTDARIAGFAGIAMCAAFMAVSS
                     LVLLVFRDQIIRFYTDDPVVTGIALSLLLVAAIFQIADGVQISAAAALRAFKDTRVTM
                     AISVFAYWIVAFPLAYLATITYQLPANNIWAAFIAGLGVAAVLLTWRF"
BASE COUNT          327 a          639 c          656 g          456 t
ORIGIN      
        1 cgtcattgaa cggtaccacg aggtcttcgt agaaaccttc gtcataggcc gcggcagctt
       61 tcatgtgact ggcaagtgcc agctggtcct ggtgctcgcg ctgcacatcc cattccttgg
      121 ccgtaagttc gcagctttcg cccatcgaca ggcccgtccg cggttcggtt accccaggga
      181 gctcgggctt gaggaaaccc ggcctaaagc ggaacagtgg cttgatgcga tccattaatg
      241 aatgcgcggc atgcatgtcg agcagcatgt ggcgatacgg gtcggggtag acgattggcg
      301 catcgctgac cgtgtcggtg ccaccggcga tgccagcgtc aatctgaccg agggcaatct
      361 tgttcgctat ctggatggcg gcttcgatgc tggtgccgca ggcgcgctgc aggtcgaagg
      421 ccggggtccg aatggaaagt cccgactcga tgacgcattc gcgcgcgaga ttccagtcgc
      481 gggagtgctt gacgacggcg ccgagggcga cttcgccgag tgtctgtccc ttgaggtcca
      541 gcttgttgat gagaccgtcg agcgcccccg tcatcaggtc ctggttggac agtccgcggt
      601 acatcgagtg agagcgacaa aaaggaatac gcgctccgcc tactacagct acccgaatgg
      661 cgtgtccgtc atgcagcatg ctttgatctc cttcttgtcc aggcgcgcaa gaactttaca
      721 ccattcgtgc gtcccgccct aggttaagat gcgccgatgt tcgcgacttc ccccgccacc
      781 cgcagacagg tttcatcgat cgcccggatc agcggtccgc tgatcatcaa tttcctggcg
      841 gttgccggca tgaacctggc cgacgtgatg atgtcgggcc ggctcggcgc ggatgcgctt
      901 gccgccgtgg cggtggggtc gagcagctgg atgttggcat tttcggcctg tctaggtctg
      961 ctcatggcgc tttcgccgat catctcgcgc ctgtacggcg ccggcgagat gcacaagata
     1021 gggcgctatg cgcgccatgg aatgtacctg gggctagggc tcggcgtggt gatcctgctg
     1081 gtcgggcgct ccaatgccca ggcgctgctg acgatgatcg gcatcgaccc ggaattccgg
     1141 ccgctcgcca tcacttacgt cagggcgctg ctgttcggcg cccccggcat cctgatcttt
     1201 atcgccctgc ggttcacgac cgagggaatc ggctatacgc gtccggtgat gttcacgtca
     1261 atcttctccc tggcctgcaa cgtgttcctc aactacacgc tgatgttcgg caacttcggt
     1321 gcgccggcca tgggcgtcga gggttgcgcg tatgcgagcg ccattacgat gtggctcgta
     1381 gcctgcgcgc tgggtttgta cgtgtcggtc agcccgaagc tgaaacccct caaggtcttc
     1441 acccgcatcg ggcagttccg gccgaagctg tttcgcgaga tcattgcgct tggcgtcccg
     1501 atctcgatca cgatcacggc cgaagtcggc ctgttctcgg ttgtgtccgt catggtcggg
     1561 acgcgtggcg tcgacattac ggccgctcac cagatcgcgc tcagctacgc gtcgacgatg
     1621 ttcatgatcc ccctggcgct gtcctccgcg acgaccgtgc aggtcggcca tatgctgggc
     1681 gccgggcgcg ataccgatgc gcgcatcgcg ggctttgccg gtatcgcgat gtgtgccgcg
     1741 ttcatggccg tgtcatcgct cgtgctgctc gtcttccgcg accagatcat caggttctac
     1801 accgacgacc cggtcgtaac cggcattgcc ctcagcttgc tgctggttgc ggcgatattc
     1861 cagattgcgg atggagtgca gatttcggcg gcagccgcgc tgcgtgcctt caaggacacg
     1921 cgtgtgacga tggcgatcag cgtgttcgcc tactggatcg tcgcgttccc gctcgcctac
     1981 ctcgcgacga ttacgtacca gctgccggcg aacaatatct gggcggcgtt catcgcgggg
     2041 ctcggcgttg ctgcggtgct gctgacctgg cgattcaa
//
LOCUS       DATDAA010000295         2258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_265998,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000295 DATDAA010000000
VERSION     DATDAA010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265998"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VIS73_09185"
     CDS             <1..1330
                     /locus_tag="VIS73_09185"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY5763368.1"
                     /translation="VYARIAELPAEQRAAVETDIEAVYARRPPLAMVNSDKGITNLHV
                     PSDVIIDASMPAALRSSGKMWGPDGKLADTHAMIPDRCYAGIYQAVFDDCREHGAFDV
                     PTMGNVCNVGLMAQKAEEYGSHDKTFEIPVAGTVRVVDEAGAVLLEHAVEPGDIWRMC
                     QTKDLPIRDWVKLAVNRARATGSAAIFWLDPYRAHDANLIAKVDEYLGRHDTAGLDIR
                     VMSPVEAMKATLERVRNGEDTISVTGNVLRDYLTDLFPILELGTSAKMLSIVPLLAGG
                     GLYETGAGGSAPKHVQQFVAEGHLRWDSLGEFLALAVSIEDLAEKSGNAKARVLAKAL
                     DKANAAFLENNKAPSRKVNELDNRGSHFYLAMYWAKAMAEQTADPELAASFAPLAEAL
                     AAREEAILGELIDCQGAPVDIGGYYHPDEARTSAAMRPSATLNAALAALA"
     gene            complement(1363..2133)
                     /locus_tag="VIS73_09190"
     CDS             complement(1363..2133)
                     /locus_tag="VIS73_09190"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEY5763369.1"
                     /translation="MRARALATIALMLLPALVSAQGYWSREGEIDTDPNAYAEFAFTR
                     VIFESGYQALPGPGNTAWRDWPESDMHFIRGINRLTAVDIEDRSRAIRLTSEQLFDRP
                     WIYALEVGTWLLSREEADNLREYLLRGGFLVVDDFHGSYQWLGFMRSMKLVFPDRAIV
                     DVSEEDAIMSMHFEVDHSEPIPGIMALRYGVTYEHDGYTPTWKGVYDDEGRLMVIINF
                     NVDLGDAWEMADEPWYPEKYTATAYRYGVNYVLYAMTH"
BASE COUNT          413 a          734 c          727 g          384 t
ORIGIN      
        1 cgtttacgcg cgcattgccg aactgcccgc tgagcagcgc gcagcggtcg aaaccgatat
       61 cgaggccgtc tacgcgaggc gtccgccgct cgctatggtc aattcggaca agggcattac
      121 gaacctgcac gtgccgagtg acgtcatcat cgacgcgtcg atgcccgccg ccttgagatc
      181 gtccggcaag atgtggggac ctgatggcaa gctcgcggac acgcatgcaa tgatccccga
      241 tcgctgctat gccggtatct accaggcggt gttcgatgat tgccgtgagc acggcgcgtt
      301 cgacgtgccg acgatgggca acgtatgcaa cgtcggcctc atggcgcaga aagccgagga
      361 atacggttcg cacgacaaga ccttcgaaat tcctgtagcc ggcaccgttc gggtcgtcga
      421 cgaagccggc gccgtgctgc tcgagcatgc cgtggaaccc ggcgacatct ggcgcatgtg
      481 ccagaccaaa gacctgccga tacgggactg ggtcaagctc gcggtaaacc gggcgcgcgc
      541 gaccggctcg gccgccatct tctggctcga cccttaccgg gcccacgatg ccaacctgat
      601 tgcaaaggtc gacgaatacc tcggtcgcca cgacaccgcc ggcctggaca tacgggtcat
      661 gtcgccggtc gaggcgatga aggccactct ggagcgggtc cggaatggcg aggacacgat
      721 ctcggtgacc ggcaacgtgc tgcgcgatta cctgaccgat ctgttcccga tcctcgaact
      781 cggcaccagc gccaagatgc tgtcgatcgt gccgctgctc gccggcggcg gactctatga
      841 aaccggcgcg ggcggctcgg ccccgaagca cgtgcagcag ttcgtcgccg aaggccacct
      901 gcgttgggac tctctcggcg aatttctcgc gctggcggta tccatcgaag acctcgccga
      961 gaaatcgggc aatgcgaagg cgcgcgtcct ggcaaaggcg ctcgacaaag ccaatgcggc
     1021 attcctcgag aacaacaagg cgccgtcccg caaggtcaat gaactcgaca atcgcggcag
     1081 ccatttctac ctggcgatgt actgggcaaa agccatggcc gagcagaccg cggatcccga
     1141 gctggcagcg agcttcgcgc cgctggcgga agcgctggcc gcccgggagg aggccatcct
     1201 gggggaactg atcgactgcc agggagcgcc ggtcgatatc ggcggctact atcatcccga
     1261 cgaggccagg accagcgcgg ccatgcggcc cagcgcgacg ctcaacgcgg cgctggcggc
     1321 gctggcctga agcgaaccgg gccgcggcgc cggcgcgccg cctcagtgcg tcatggcgta
     1381 gagcacgtag ttgacgccgt aacggtaggc cgtcgcggtg tacttctcgg ggtaccaggg
     1441 ctcgtcggcc atctcccacg cgtcgcccag gtcgacgttg aaattgatga tcaccatcag
     1501 gcggccctcg tcgtcgtaga cgcctttcca cgtcggagtg tagccgtcgt gctcgtaggt
     1561 cacgccatag cgcagcgcca tgatgccggg aataggctcg gagtgatcga cctcgaaatg
     1621 catgctcatg atcgcgtctt cctcgctgac gtcgacgatc gcccggtccg ggaacaccaa
     1681 tttcatcgag cgcatgaatc ccagccactg gtaggagccg tgaaaatcgt cgacgaccag
     1741 gaagccgccc cgcagcaggt actcgcgcag gttgtccgct tcttcccggg acaaaagcca
     1801 ggtgccgacc tcgagcgcat agatccacgg ccggtcgaac agctgctcgc tggtcaggcg
     1861 aatggcgcgg ctccggtcct cgatgtcgac ggccgtcagc cgattgatgc cgcggatgaa
     1921 atgcatgtcg gattccggcc agtcccgcca ggccgtgttg cccggccccg gcagcgcctg
     1981 gtagccggat tcgaaaatga cgcgcgtgaa cgcgaactcg gcataggcgt tggggtcggt
     2041 gtcgatctcg ccctcccggc tccagtatcc ctgggccgag accaacgcgg gcagcagcat
     2101 cagggcgata gtggcaagcg ctcttgctct catcccggtg cggatcgctc ctcggcgcgg
     2161 cgggttggtt gcgtcatttc gccagcgggt ccggacgcac ctggaaatgc acgacgattg
     2221 gcttttgtcc cttgccgcct tccttcagcc atggcgtc
//
LOCUS       DATDAA010000296         2519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_336553,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000296 DATDAA010000000
VERSION     DATDAA010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336553"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VIS73_09195"
     CDS             <1..822
                     /locus_tag="VIS73_09195"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEY5763370.1"
                     /translation="ALFITLVALVINSAFTGDFSTAVSFLFTADLSKITPYVVLAAVG
                     QAFFSVNVGVGAVLTYSAYLPKDVNLFRSAIIVAMGDTLVALLAGLAIFPIVFANGLD
                     PAEGPGLIFVTLSTAFAQMPGGSIIGTGFFLMILFAALTSSISMLETMTARIFEIRGI
                     TRTAAASMIGAGTFLLGLVTVFSFSTWENTFPLGQFEVFATKTPFDLIDYLVSNVLMP
                     LGGMLYAIFAGWWLSRQTLLSEIGLGDGALFKLWMVLVRIIAPLAVAAVFVFNLT"
     gene            908..2203
                     /locus_tag="VIS73_09200"
     CDS             908..2203
                     /locus_tag="VIS73_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HEY5763371.1"
                     /translation="MSDKKKFLFVSLDALISDIAWQVRAGGHEVRYFIASESERDIAD
                     GFVPKVDDWESHVEWADVIVFDDVMGQGEHAARLRSEGKAVIGGTPYTDRLEDDRTFG
                     QQELKKHGISIIPFQEFTDFDSAIEYVEANPSAYVIKPSGEAQNIKRLLFVGREDDGK
                     DVIHVLESYKAAYADTVKVFQLQRRVSGVEVAVGAFFNGREFMLPINVNFEHKPLFPG
                     NIGPATGEMGTCMYWSGRNRLFSATLAKLEDRLREEGYVGYIDVNCIVNGHGIYPLEF
                     TARFGYPTIFIQADGINMPIADFLAGMAEGSLRDFKTRKGFQIGVRVVVPPFPYNDPK
                     TVAANSKDRVIIFRRPFNEGIHIEDVKMVNGEWLITGHSGVVLVVTGMGISVKQAQAQ
                     AYQRIRNILIPNMYYRTDIGDRWVEDHDKLHSWGYLRES"
     gene            complement(2222..>2519)
                     /locus_tag="VIS73_09205"
     CDS             complement(2222..>2519)
                     /locus_tag="VIS73_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763372.1"
                     /translation="IPSGPPSREAYSVSNLKGILRWTTWAEKCFRFWAGQNSDCSICI
                     RVCPYNRDFSKWYNRLWRRLAGTRLRKLMLGLDVWLVKRERKSSAAWWRAGGNS"
BASE COUNT          449 a          814 c          768 g          488 t
ORIGIN      
        1 gcgctgttca ttactctcgt cgccctcgtc atcaactcgg cgttcacggg cgatttttcg
       61 accgcggtca gcttcctgtt cacggccgat ctcagcaaga tcacgcccta cgtcgtgctc
      121 gccgccgtcg gccaggcctt cttctcggtc aacgtgggcg tcggcgccgt gctgacctac
      181 tcggcctacc tgccgaagga cgtgaacctg tttcgctcgg ccatcatcgt cgccatgggc
      241 gacacgctcg tcgcgcttct ggccggcctg gccatcttcc cgatcgtctt cgccaacggc
      301 ctggatccgg ccgaggggcc gggcctgatc ttcgtcacac tttcgacggc cttcgcgcag
      361 atgcccggcg gctcgatcat cggcacgggt ttcttcctga tgatcctgtt cgcggcgctc
      421 acctcgtcga tatccatgct cgagacgatg acggccagga tcttcgaaat ccgcggcatc
      481 acccggacgg ccgccgcatc catgatcggc gccggcacct tcctgctcgg gctcgtgacc
      541 gtattctcct tctcgacctg ggagaacacc ttcccgctcg gccagttcga ggttttcgcc
      601 acgaagacgc ccttcgatct gatcgattac ctcgtgtcga acgtcctgat gccgctgggc
      661 ggcatgctgt acgctatttt cgccggctgg tggctttcga ggcagacgct tctgtccgag
      721 atcggtctcg gggacggcgc cttgttcaag ctctggatgg tgctggtgcg cattatcgcg
      781 ccgctggcgg tcgcggcagt tttcgtcttc aacctgacct gattttttcg ttgacgcggc
      841 cccgattcgg gcgcctaatg cgcgccttcg cacagcgtgc gctcgtcaat ttcgggggct
      901 tttggccatg tccgacaaaa agaaattcct cttcgtctcg ctcgatgcgc tgatcagcga
      961 tatagcctgg caggtgcgcg cgggggggca cgaggtccgc tacttcatcg ccagcgaatc
     1021 ggagcgcgac attgccgacg gattcgtgcc caaggtggac gactgggagt cgcacgtcga
     1081 gtgggccgac gtcatcgtgt tcgacgacgt aatgggccag ggcgaacatg cggcgcggct
     1141 gcgcagcgaa ggcaaggccg tcatcggcgg cacgccctat accgaccggc tcgaggacga
     1201 ccggaccttc ggccagcagg agctgaagaa acacggcata tcgatcatcc ctttccagga
     1261 attcaccgat ttcgacagcg cgatcgaata cgtggaggcc aacccttcgg cctacgtcat
     1321 caagccgagc ggcgaagcac agaacatcaa gcgcctgctg ttcgtcggcc gcgaggacga
     1381 cggcaaggac gtcattcacg tgctcgagtc ttacaaggct gcgtacgcgg acaccgtcaa
     1441 ggtcttccag ctgcagcgcc gcgtcagcgg cgtcgaggtc gccgtgggcg cgtttttcaa
     1501 cggcagggag ttcatgctgc cgatcaacgt gaacttcgag cacaagccgc tctttcccgg
     1561 caacataggc ccggcgacgg gcgagatggg cacctgcatg tactggagcg ggcgcaaccg
     1621 cctgttctcg gccacgctcg ccaagctcga ggacaggctg cgcgaggaag gctacgtggg
     1681 ctacatcgac gtcaactgca tcgtcaacgg ccacggcatc tatccgctcg aattcacggc
     1741 gcgtttcggt tacccgacga tcttcatcca ggcggacggc atcaacatgc cgatcgccga
     1801 cttcctcgca ggcatggcgg aaggcagcct gcgcgatttc aagacgcgca agggcttcca
     1861 gatcggagta agggtcgtcg tgccgccttt cccgtacaac gacccgaaga ccgtcgcggc
     1921 gaactccaag gatcgcgtca tcatcttccg caggcccttc aacgagggca tacacatcga
     1981 agacgtcaag atggtcaacg gcgaatggct catcaccggg cacagcggcg tcgtgctggt
     2041 cgtcaccggc atgggcatct ccgtcaagca ggcgcaggcg caggcctacc agcgcatcag
     2101 gaacatcctg attccgaaca tgtactaccg tacggacatc ggcgaccgct gggtcgagga
     2161 ccacgacaag ctgcattcgt ggggctacct gcgggagtcg tgaacggcgg ccgcgaaggt
     2221 ctcaggaatt gccgcccgcc cgccaccacg cggccgagga ttttcgctcg cgcttgacca
     2281 gccagacgtc gagcccgagc atcagtttgc gcaggcgagt gccggcgagc ctgcgccaca
     2341 ggcggttgta ccacttggaa aagtcgcggt tgtacgggca aacccggatg cagatcgagc
     2401 agtcggaatt ctgccccgcc cagaagcgaa aacatttttc cgcccatgtc gtccagcgca
     2461 gaatgccctt gaggttggag acgctgtagg cctcgcgcga gggcggtcct gacgggatg
//
LOCUS       DATDAA010000297         6163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_302280,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000297 DATDAA010000000
VERSION     DATDAA010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302280"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VIS73_09210"
     CDS             complement(<1..187)
                     /locus_tag="VIS73_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="signal protein"
                     /protein_id="HEY5763373.1"
                     /translation="MGLRLKFNLVLLGVFLVGFAATGAVSYRLLRSNAEDEVLRAGQL
                     MMENALAIRGYTVSQIKP"
     gene            complement(266..1414)
                     /locus_tag="VIS73_09215"
     CDS             complement(266..1414)
                     /locus_tag="VIS73_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763374.1"
                     /translation="MWRIAGEVLAQDTRGPARRLQAGDSVFVGERISASATGEAVLKT
                     SDAGIIAVRPGAVFAAERFVAEGGRRDSATYRIFTGALRVLTGWIGRLNSREQRIATP
                     TATIGIRGTDHEPYVLTDDLAVRLVQKAGTYDKVNSGGTTLEAGAGSVDIDPGRVGFA
                     KAPPRSKDRALMTLALPVLLDRIPDFYVPGRFDDEIDRLATTLKAEAASALERQRQAA
                     PADGGGAAALKLREPSPLELTGRARRDDVCNANQVARQWLGELDRGIAAGKVNDILRL
                     FDAKLVLRVNARSRDGSQSVIELPRDEFARSAAAAVANLSEFKQQRPNTIARPLEIGR
                     CDRLSVRSVSIEQGRQGGTPYRFESLEEYVLEQRGQDWIAIKAESTQR"
     gene            1892..2437
                     /locus_tag="VIS73_09220"
     CDS             1892..2437
                     /locus_tag="VIS73_09220"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEY5763375.1"
                     /translation="MVALLLPAVLVRDRNRWRGADRFRDAVSEVLWVVTAFTVAHSIT
                     LSLAALSVVTLPSRLVESVIALSVALAAANNLRPVVARRRWLVALGFGLIHGFGFASV
                     LADLGLPSESLLLSLMGFNLGVELGQLAIVSGFLPLAFLLRRTPFYRRVVFIGGSLVA
                     LAVSLVWFFERATGHTIYWPG"
     gene            2665..3591
                     /locus_tag="VIS73_09225"
     CDS             2665..3591
                     /locus_tag="VIS73_09225"
                     /inference="COORDINATES: protein motif:HMM:NF006452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEY5763376.1"
                     /translation="MNTSESIASQPLFRDYTQFRVRFEPEDGVLRVSMQGRPVPCYSE
                     ALLEEARAIQCSIEAGGGLIEHAGEMRRLEYVVLDSQVPGVFNLGGDIALFRRLIESR
                     DRDGLTRYARLCVDVCHRNIVHYNLPCLSISLVAGKALGGGMESALSGNVVIAERSAE
                     FGLPEVLFNLFPGMGAYNLIARRIGPRLAEELITSGRTYSGEELHRMGLVDVLANDGQ
                     GEKALEEYLHANKRKQKTLHALAAVRETLSPVTHEDLLKVVPIWVESALGLDARDLKV
                     MERLVKAQSRAFVEPTPAGVTTAGASVRMSLA"
     gene            complement(3568..6039)
                     /locus_tag="VIS73_09230"
     CDS             complement(3568..6039)
                     /locus_tag="VIS73_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5763377.1"
                     /translation="MTADPIAKPVDGQAQPARLKDVEFEQALLRTIVVGILLIYLLVK
                     SALDASGIDEITVPLAFAYFAAALVLMITVHLRPGAYPARRLLGMVLDNSGATACLWL
                     AGDAGAVAIGVYLWVAFGNGFRYSRRYLFASQAMAVVGFSSVLAFTPHWQEHPYLGAG
                     ILVMLLVLPIYVATLIARLQDALARAEVANEAKTRFVSNVSHELRTPLNGIIGMNEVL
                     KAKKLTPEQRDIVLTLGSAAKAMAKLVDEILDLSRIEAGKLVIAREPMDLHRVVGSVS
                     KLMRNQAEAKGLRLTLNIASDVPFAVVGDESHLRQVLLNLVGNAIKFTEQGEVRINIT
                     TIGDDGPRVGVRFDVIDSGVGIDPAHHHRIFERFAQADNSTIRRYGGTGLGVTIAKHL
                     VEAMDGEMGLISKVGAGSTFWFRLDFERVQAEPPGSEFSGVGVLLLAPASDRSFSDIA
                     VALARSGAFTRFCNASGEVVEAVEYHRDIYQWVVVVSNDGTSEDALTLPRSLRRELKE
                     HRISFIGCSGTSPIDTLGALGQGYSYAFNRRTRPETVVNAIHACFAEAERATDPVTQL
                     AAPANVLPALLVDDNETNRTVAKLLLENAGYRVVTACDGDEALDLLGSRDFSVILLDM
                     HMPGMSGLDVLKTYRFMSQPGRRAKVIILSADTTPKLMQETIAAGANAYLMKPIDREQ
                     LLRTLHTLLGGGAPVDAAPEKPAEEVLDYALLGSLAELSPGGNFMHDLITGFLTDAEE
                     LLARIDEAVSEQDHAAFADRIHALKGSAGNIGAIVLFKACAAARTLSPKAFRADGAKA
                     AGVLRDCLERSAEALNAYLGQRHPN"
BASE COUNT          994 a         1891 c         2081 g         1197 t
ORIGIN      
        1 cgggcttgat ctggctgacc gtgtagccgc ggatcgccag cgcgttttcc atcatcagct
       61 ggccggcacg aaggacctcg tcctcggcgt tgctgcgcag cagtcggtag gaaacggcac
      121 cggttgccgc gaagccgacg agaaacacgc cgagcagaac caggttgaac ttaaggcgca
      181 gacccatgct gtactccccc caggggttgg cttcttgctg aagattgacc ggcgcgccgg
      241 cgcgtagttc gcgtctgcaa ggcggtcagc gctgcgtcga ctcggctttg atcgcgatcc
      301 agtcctgtcc gcgttgttcg agtacgtact cttccaacga ttcgaaacgg tagggcgtgc
      361 caccctggcg gccctgttcg atcgacaccg agcgcaccga cagccgatcg caacgcccaa
      421 tttccagcgg tcgggcaatg gtgttggggc gctgctgttt gaactcgctg aggttcgcga
      481 ccgcggcggc cgcgcttcgc gcgaactcgt cacgtggcaa ttcgatcacg ctctgcgagc
      541 catcacggct gcgtgcattg acgcgcagga ccagcttcgc gtcgaacagg cgcaggatgt
      601 cattgacctt gcccgcggcg atgccgcggt cgagttcgcc aagccactga cgggcaacct
      661 ggtttgcgtt gcaaacatcg tcgcggcggg cgcggcccgt caattcaagc ggcgagggct
      721 cgcgcagttt cagcgcagca gcgccgccgc catctgccgg tgcggcctgg cgctgtcgct
      781 cgagcgcgct tgccgcttcg gctttcagcg ttgtggcgag tcggtcgatc tcgtcgtcga
      841 agcggcccgg cacgtagaag tcgggaatgc gatcgagcag caccggcagc gccaacgtca
      901 tcagcgcccg gtccttgctg cgcggcggtg ccttggcaaa accgactcgg ccgggatcga
      961 tgtcgacgct gccagcgccc gcttctagtg tcgtgccgcc actattgacc ttgtcgtagg
     1021 tgccggcctt ttgaacaaga cgcaccgcga ggtcgtcggt cagcacatag ggttcgtgat
     1081 cggtaccgcg aatgccgatg gtggccgtcg gggtggcgat tcgctgttcg cggctgttga
     1141 gtcggccgat ccagcccgtg agtacccgga gggccccggt gaagatgcga taggtcgcgc
     1201 tgtcgcggcg gccgccttcg gccacaaaac gctcggcggc gaagacggca ccgggtcgga
     1261 cggcgatgat tccggcgtcg gaagtcttga gcaccgcttc gccggtggcc gaagcgctga
     1321 tccgttcgcc gacgaatacc gaatcgccgg cctgcagacg acgcgctggg cctcgtgtgt
     1381 cttgtgcgag tacctcgccc gcgatacgcc acacggatgc gaacggccgg ggcgactggg
     1441 gatcagcagc cgccgcctgc ggcggaaagc cgatacatcc cgcggtgagc aggacgaggc
     1501 ctgcgagtcg cgccagcagg cgtttttcgg gagtggccac aatcatgatt cgtgggctca
     1561 gcagatgtgg gcgagcggcg attgaaacat gatccgtcac taggcaccag aacggcggcg
     1621 cctacaccgt cctgcgaatc gccggcgaat gcccgtcggg cagtggagtg gtgacctcga
     1681 catcgattac cgcctgctgt tcgaactcga tgcgaatcac cgtggcttgc taaatctcga
     1741 tctcgacggg acgatccata gcgcagtttt tagccccgat cggactcagc agcgattcgc
     1801 cgccggcgga tcgtcgcggc tcggttagtt cggccagtat cttggtgacg gggtttggca
     1861 tatctggatc ggcatcgacc acatcctctt tctggttgcg ctgctgctgc ctgccgttct
     1921 ggttcgcgat cgaaatcgct ggcgaggtgc cgaccgattt cgggacgcgg ttagcgaggt
     1981 gctgtgggtg gtgactgcgt tcaccgtcgc ccattcgatc acgttgtcct tggccgcgct
     2041 cagcgtggtc acgctgccgt ctcggctggt cgaatcggtc attgcgctct cggtcgcctt
     2101 ggccgcggcc aataacctgc ggccggtcgt cgcgcgccga cgctggttgg tcgcgcttgg
     2161 cttcggcctc attcatggct tcggctttgc cagcgtgctc gccgatctcg gcttgccgag
     2221 cgagtccttg ctgctttcac tgatgggctt caacctcggc gtcgagctcg gtcagctggc
     2281 catcgtctcg ggttttctgc cgcttgcctt cctcttgcgc cggacgccgt tttatcgtcg
     2341 ggtggtcttc attggcggtt cgctggtagc gctcgcggtg tcgctggtct ggttcttcga
     2401 gcgcgccacc ggccacacga tctactggcc gggctgactc cgagtccggt tcgctgccgg
     2461 cgcaatggcg gctcgggtat ttacgcgcgc gggcgtcccg gctaatctcg ttgccagccg
     2521 tttagcgatc gtcaaatgtt ccggtggttt ccgacgcgcg gcgtcgctgt ttggggtgct
     2581 gcgtcggtac ggcaatcgtc acgacagaac gacggtttat ttgtctgtgc cgattcgtcg
     2641 gcgcgtccgt tttccggagg tcccatgaac accagcgaat ccattgccag tcagccgctt
     2701 ttccgtgact acacccagtt tcgcgttcgt ttcgaacccg aagacggtgt gcttcgggtg
     2761 tcgatgcagg ggcggccggt gccttgctac tcggaggcgc tgctcgaaga ggcgcgggcg
     2821 atccagtgtt cgatcgaagc cggtggcggc ctgatcgaac acgctggcga gatgcgccgg
     2881 cttgagtacg tcgtgctcga ctcgcaagtt cccggggttt tcaatcttgg cggtgacatc
     2941 gcgctgttcc ggcggctgat cgaatcccgc gaccgcgacg gcctgacgcg ttatgcgcgc
     3001 ctgtgcgtcg acgtctgtca ccgcaacatc gttcactaca acctgccgtg cctgtcgatt
     3061 tcgctggtcg ctggcaaggc gctcgggggc ggcatggaat ctgccctctc gggaaacgtg
     3121 gtgattgccg agcgcagcgc cgaatttggc ctgcccgaag tgctgttcaa cctgtttccg
     3181 gggatggggg cgtacaacct gatcgcccgg cggattggtc cgcgcttggc cgaggaattg
     3241 attaccagcg ggcgtaccta ttcaggtgag gagttgcacc ggatggggct cgttgacgtg
     3301 ctggccaacg acgggcaggg tgagaaggca ctcgaggaat acctgcacgc caacaagcgc
     3361 aagcagaaga cgctgcatgc cctggcggcg gttcgcgaga cgctgtcgcc ggtgacgcac
     3421 gaggatctgc tgaaagtggt gccgatctgg gtcgagtcgg cgctcgggct cgacgcgcgc
     3481 gacctcaagg tgatggagcg tctggtcaag gcgcagtcgc gcgctttcgt cgaaccgaca
     3541 ccggctggcg tcactaccgc cggggcttca gttcggatgt cgttggccta ggtacgcgtt
     3601 cagagcctcg gcgctacgct cgaggcaatc gcgcaagacg ccggccgcct tcgcgccgtc
     3661 tgcgcgaaac gctttggggc tgagcgtgcg cgcagcggcg caggccttga acaggacgat
     3721 cgcgccgata ttgccggcgc ttcccttgag cgcgtgaatg cggtcggcaa aggcggcatg
     3781 gtcttgttcg ctgacggcct cgtcgatgcg tgccagcagt tcttcggcat cagtgagaaa
     3841 gccggtgatc aggtcatgca tgaagttgcc gccgggtgac aactcggcaa ggctgcccag
     3901 cagggcgtaa tcgagaactt cctcggcggg tttttcaggt gccgcgtcta ccggcgcgcc
     3961 gccacctagc aacgtgtgca gcgttcgcag caactgctcg cgatcgatgg gtttcatcag
     4021 gtaggcgttg gcgccggcgg cgatggtttc ctgcatcagt ttcggcgtcg tgtcggccga
     4081 gaggatgatc accttggccc gccgaccagg ctggctcatg aagcggtagg tcttgagcac
     4141 gtcgaggccg ctcatgccgg gcatgtgcat gtcgaggagg atcaccgaga agtcgcgtga
     4201 gccgagcaga tcgagcgcct cgtcgccatc gcaggccgtg accacgcgat agccagcgtt
     4261 ttcgagcagc agcttggcga cggtacggtt ggtctcgttg tcgtctacca gcaatgccgg
     4321 cagaacgttg gccggcgcgg ccaactgggt caccgggtcg gtggcgcgtt cggcttcggc
     4381 gaagcaggcg tggatcgcat tgacgacagt ttccgggcgg gtccgccggt tgaaggcgta
     4441 gctgtagccc tgaccgagcg caccgagcgt gtcgatcggc gaggtgccgc tgcagccgat
     4501 gaaggaaatc cggtgttcct tgagttcgcg acgcagcgag cgcggcagcg tcagcgcatc
     4561 ttcgctggtg ccgtcgttgc tgacgaccac tacccactgg tagatatcgc ggtggtactc
     4621 gactgcttcg accacttcac cggaagcgtt gcagaagcgc gtgaaggcac cgctgcgtgc
     4681 gagagcgacg gcgatgtcgg aaaagctgcg gtcgctggcc ggcgccagca gcaggacgcc
     4741 gacacccgag aactccgatc ccggaggttc ggcctggacg cgttcgaagt cgagccggaa
     4801 ccagaaggtg ctgccggcgc cgaccttgct gatcagaccc atctcgccgt ccatcgcctc
     4861 gaccaggtgc ttggcgatgg tgacgccgag gccggtgccg ccgtagcggc gaatcgtcga
     4921 gttgtcggcc tgggcgaagc gctcgaaaat gcggtggtgg tgtgcagggt cgatgccgac
     4981 gccggaatcg atgacgtcga agcggacgcc gaccctcggg ccgtcatcgc caatcgtggt
     5041 gatgttgatc cgcacctcgc cctgctcggt gaacttgatc gcgttgccaa cgaggttaag
     5101 cagtacctgg cgcaaatggc tctcgtcgcc gactaccgcg aacggtacgt cggaggctat
     5161 gttcagcgtc agccgcagcc ccttggcttc ggcctggttg cgcatcagct tgctgacgct
     5221 gccgaccacg cggtggaggt ccatcggttc gcgggcgatt accagcttgc cggcctcaat
     5281 gcgtgacagg tcgagaatct cgtcaaccag cttggccatc gccttggctg ccgagcccag
     5341 cgttaatacg atgtcacgtt gctcgggcgt cagcttcttc gccttgagca cctcgttcat
     5401 gccgatgatg ccgttgagcg gcgtgcgtag ttcgtggctg acgttggaga cgaagcgcgt
     5461 cttcgcttcg ttggccactt cggcgcgggc cagcgcgtcc tgtaggcgcg cgatcagcgt
     5521 cgcgacgtag atgggcagga ccagcagcat gaccaggatg ccggcaccta gataagggtg
     5581 ctcctgccaa tgcggggtga aggcaagcac gctggaaaag cccacaacgg ccatggcctg
     5641 cgaggcgaac aggtagcggc ggctgtagcg gaagccgttg ccgaaggcga cccagaggta
     5701 aacaccgatg gcgacggcgc cggcatcgcc ggcgagccac aggcatgccg tcgcccccga
     5761 gttgtcgagc accatgccga gcagccggcg cgccgggtac gctcctggcc gcaggtggac
     5821 ggtaatcatc aataccagcg ccgcggcgaa ataggcgaag gccagcggta ccgtgatttc
     5881 gtcgatgccg ctggcgtcca gcgcggattt caccagcaga tagatgagca ggatgccgac
     5941 gacgatggtc cgcagcagcg cctgctcgaa ctcgacgtcc ttcagtcgag ccggctgcgc
     6001 ctgcccgtcg acaggtttgg cgatggggtc cgcagtcatt ggaacgactc gatgatctgg
     6061 cggacttccg actgacgatc gaggaccgcg cgcacgcaca gcggatcaaa cgacacgcca
     6121 gcctgctcgg caatgtaggc aaaggcatcg gcaatcggcc acg
//
LOCUS       DATDAA010000298         3159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_372141,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000298 DATDAA010000000
VERSION     DATDAA010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372141"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1022)
                     /locus_tag="VIS73_09235"
     CDS             complement(144..1022)
                     /locus_tag="VIS73_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5763378.1"
                     /translation="MTATTPFHCERIDLRGLGYAVRHWGPEGAPQLFFLHGWLDTSAT
                     FQFVVDALRKDWHVIAPDWRGHGDSEWLDRAYWFPDYYADLDALLSRYSPNAPARLVG
                     HSMGASIAGTYAGVRPDRVAQVVMMDFLGLPPSTADEAPARLAQWLDAVAEPPKPRHY
                     PDHDAFAHRLMKSNPRLTDERAAFLARHVTRTHADGSVTLACDPWHRVPAPVRYQTED
                     VLAGWRRISAPVLNLLAAEGFVIDRFGRQPEELARRLGCFQNQREQTLADCGHNLQHD
                     QPEQVAAAIEAFLAHV"
     gene            complement(1178..1253)
                     /locus_tag="VIS73_09240"
     tRNA            complement(1178..1253)
                     /locus_tag="VIS73_09240"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1218..1220),aa:Val,seq:tac)
     gene            complement(1348..1833)
                     /locus_tag="VIS73_09245"
     CDS             complement(1348..1833)
                     /locus_tag="VIS73_09245"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HEY5763379.1"
                     /translation="MRITIFTTGGTIDKVYFDAKSEFHVGDSEIPEFLRVASVVLPTQ
                     IESLLRKDSLDVDDADRAMIRERVIDESASRILITHGTDTMVETARVLAGIPDKTIVL
                     TGAMRPSRQRVSDAVFNIGFALAAVQLLPPGVYLAMHGEILDPEKARKNRAAGRFESA
                     G"
     gene            complement(1830..>3159)
                     /locus_tag="VIS73_09250"
     CDS             complement(1830..>3159)
                     /locus_tag="VIS73_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEY5763380.1"
                     /translation="ACREASVEVEALLYCPDYRVRNPGTAGIDPARIIDAPRREALLP
                     TIRAILPEANDPGTGNDPAVHRFLADLLELVADVTAVVGEAEALTTRLSGGLADWARR
                     IECRPQRLRVTGTAGSGKTQLALAVYRDAVNAGRRPLYVCYNRPLAEHIRAIVPAGGE
                     VASYHQLADQISRRLGQTPDFSQAGAFAALEAVLDRYQPDHDQRFDVLIVDEGQDFKP
                     DWCANLLRLLHADGSAWWLEDPMQNLYGRAPVPLADWVHLRADTNYRSPQDIVDALNR
                     LLPFAKPLESGCPIAESGIDVLSYRDADELLTRTVGAVTRAIGAGFRRQQIAIVTYRG
                     REHSAFTGRTRIGPYPLIAPTGEYDLFGNPVLTSGDVIIDSVHRFKGRAAACVILTEI
                     DFAELDEQATRKLFVGATRARLKLILVIAEASENLLKSRLDDAIQQAEAT"
BASE COUNT          508 a          938 c         1143 g          570 t
ORIGIN      
        1 caaggccggc gatgtccggc cttgttcttt cttgccggcg ggtcatcagt ctgccgatta
       61 gtcaccccgt ggcggtgcag gcgccgtcag ccgggccgcg gcgtgacggg tggcttgtgg
      121 gcttcggcga gctggttggc ctatcaaacg tgcgccagaa atgcctcgat tgccgccgcc
      181 acttgttcgg gttgatcgtg ctgcagattg tgtccgcagt cggcaagcgt ctgctcgcgc
      241 tgattctgga agcagccgag gcgacgcgcg agctcttcgg gctgccggcc gaagcggtcg
      301 ataacgaaac cctctgccgc gagcagattc agaacgggtg cgctgatccg gcgccagccg
      361 gccagcacat cctcggtctg atagcgcact ggcgccggca cccgatgcca aggatcgcag
      421 gccagcgtga cgctgccatc ggcgtgcgtc cgggtgacgt gccgtgcgag aaacgcggcg
      481 cgctcgtcgg tgagtcgggg attcgatttc atcagtcgat gtgcgaaggc atcgtggtcc
      541 gggtagtgcc gcggcttcgg cggttcggcg acggcgtcga gccactgcgc cagacgggcc
      601 ggtgcttcat cggccgtgct cggtggcagg ccgaggaaat ccatcatcac cacctgcgca
      661 acacgatccg ggcgcactcc ggcgtaggtg ccggcgatgc tcgcgcccat gctgtgacca
      721 acgagtcgcg cgggtgcgtt cggcgagtaa cgggaaagca gcgcgtcgag gtcagcgtag
      781 taatcgggaa accagtacgc acgatcgagc cactctgagt cgccgtgccc gcgccagtct
      841 ggcgcgatca catgccaatc tttgcgcagt gcgtcgacga cgaactggaa ggtcgccgag
      901 gtatccagcc agccgtgcag gaagaacagc tgcggtgcac cctcgggccc ccagtgccgg
      961 accgcgtagc caagcccgcg gaggtcgatt cgttcgcagt ggaagggtgt cgtggccgtc
     1021 atgggcggat ggcgggtcgg tggctttggg cgaacttcgg atcagttggg ggcgccagta
     1081 gcaggcctgt ccagccagca tacgacatgc attgcttgca gccattgcgt tcagcggaaa
     1141 agacaatggc ccgcattgct gcaggccatt gatttgatgg tgggtgctga cggggtcgaa
     1201 ccgccgacat tcgccttgta agggcgacgc tctaccaact gagctaagca cccgaatccg
     1261 ggttcgccgg cgcaagcggc ggcgagggcg cgtattagag caagcgccgc cttgtcagtc
     1321 aaggcgggcc gtgccggtgg cttcaactca accggctgac tcgaatcgac ctgcggcgcg
     1381 gttcttgcgc gctttttcgg ggtcgaggat ctcgccgtgc atcgccaggt agacgccggg
     1441 cggcagcaac tgcaccgccg cgagtgcgaa accgatattg aatactgcat cgctgacccg
     1501 ctgccgcgag gggcgcatcg caccggtgag aacgatcgtt ttgtcgggaa tcccggccag
     1561 tacccgcgcg gtctcgacca tggtgtcggt tccgtgggtg atgaggattc gcgatgcgga
     1621 ttcgtctatg acgcgttcgc ggatcatggc gcggtcggca tcgtcgacgt cgaggctgtc
     1681 cttgcgcagc agagattcga tctgcgttgg cagcacgacc gaggcgacgc gcaagaattc
     1741 cggaatttcc gagtcgccga cgtggaactc gctcttggcg tcgaaataga ccttgtcgat
     1801 ggtgccgccg gtagtgaaga tggtgattct catgtggcct ccgcttgctg gattgcgtca
     1861 tccagccgtg atttgaggag attctcgctt gcctcggcga tgacgagaat cagcttgagc
     1921 cgcgcgcgcg tggcgccgac gaagagcttg cgcgtggcct gttcgtcgag ttcggcgaaa
     1981 tcgatctcgg tgaggatgac acaggcggca gcgcggccct tgaaacgatg gaccgagtcg
     2041 atgatgacat caccggaagt cagcaccggg ttgccgaaga gatcgtactc cccggtcggc
     2101 gcgatcagcg ggtaggggcc gatgcgcgtg cggccggtga atgccgagtg ctcgcggccg
     2161 cgataggtga cgatggcaat ctgctggcgg cggaagccgg cgccgatggc gcgggtgacc
     2221 gcgccgacgg ttcgggtcag cagttcgtcg gcatcgcggt aactcagcac atcgattccc
     2281 gattcggcga tcgggcagcc cgattcgagt ggtttggcaa acggtagcag gcgattgagc
     2341 gcgtcgacga tgtcctgcgg gctgcggtag ttggtgtccg cgcgcagatg gacccagtcg
     2401 gccagcggaa ccggcgcgcg cccgtagaga ttctgcatcg ggtcttcgag ccaccaggcg
     2461 ctgccgtccg cgtgcagcag gcgcaggagg ttggcgcacc agtcgggctt gaagtcctgt
     2521 ccttcgtcga cgatcagcac gtcaaaacgt tggtcgtggt cgggctggta gcggtcaagc
     2581 acggcctcga gcgcggcaaa cgcgccggct tgggaaaagt cgggggtttg gccgagtcgg
     2641 cggctgatct ggtcggcgag ctggtgatag ctcgctactt caccgccggc ggggacgatg
     2701 gcgcgaatgt gctcggccag cggccggttg tagcagacgt agagcggccg gcggccggcg
     2761 ttgacggcat cgcgatagac ggcgagcgcc agctgggtct tgccgctgcc ggcggtgccg
     2821 gtgacgcgca ggcgctgcgg ccggcattcg attcgccgcg cccagtcggc gagtccgccc
     2881 gacagtcgcg tggttagcgc ttcggcttcg ccgacgacgg cggtgacatc ggcaacgagt
     2941 tcgagcaggt cggcgaggaa gcggtgcacg gccggatcgt tgcccgtgcc ggggtcgttt
     3001 gcctccggga ggatggcgcg tatcgtcggc agcagcgctt cccggcgcgg agcgtcgatg
     3061 atccgcgccg ggtcgatgcc cgccgttccg gggttgcgga ctcggtagtc ggggcaatag
     3121 agcagggcct cgacctccac gctggcttcg cggcaggcg
//
LOCUS       DATDAA010000299         5497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_336749,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000299 DATDAA010000000
VERSION     DATDAA010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336749"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VIS73_09255"
     CDS             <1..543
                     /locus_tag="VIS73_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763381.1"
                     /translation="PPRRILAMIHKNEARLCSVGWFRTAERENFARYSTPIHVDRPHI
                     VLANSDAWAAVSAKGALLTLMDDPDLRLGLVDGYSYGTYLDALIAARQGPTNTDSLDA
                     SQLLRMLAVRRVDFALFDREEADAMVTGNESAKALRTVFLNGMPEGQRRHLICSLQVS
                     EAEIKAINAVIAREPRTVRR"
     gene            complement(550..1173)
                     /locus_tag="VIS73_09260"
     CDS             complement(550..1173)
                     /locus_tag="VIS73_09260"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY5763382.1"
                     /translation="MAARYFRAPLKAALRRKHTAPGAELPDPGPPSDGGDDQALLAAA
                     LADVKPLPPCNRARIQKPRPAPLPRQREADEQLALRESLAEPDGPLLLEGGAEESFAQ
                     PGVSRRTLADLRRGRWIAEAVLDLHGMKRNEARQAIDAFLDDCLAQRRRVVRIIHGRG
                     LGSPGGVSILKLLSRQWLLGRAGVLAYCQARQQDGGEGAIQVLLRGY"
     gene            complement(1179..2141)
                     /gene="trxB"
                     /locus_tag="VIS73_09265"
     CDS             complement(1179..2141)
                     /gene="trxB"
                     /locus_tag="VIS73_09265"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY5763383.1"
                     /translation="MATQANRHCRLLILGSGPAGYTAAVYAARANLKPVLITGLAQGG
                     QLMTTTDVDNWPADAQGVQGPELMARFEEHARRFETEVIFDHIHTAKVGARPFTLIGD
                     SGTYTCDALIIATGASAQYLGLPSEEKFMGKGVSACATCDGFFYRNQDVAVIGGGNTA
                     VEEALYLSNIAKHVTLVHRRDTFRAEKILIDHLMAKVKEGKISLKLDATLDEVLGDDS
                     GVTGMRIKGTKDGKTEDLKLMGVFIAIGHKPNTDLFVGQLEMDGGYLITECGRNGNAT
                     QTSVAGVFAAGDVQDHIYRQAVTSAGTGCMAALDAERYLDALGS"
     gene            2368..2985
                     /locus_tag="VIS73_09270"
     CDS             2368..2985
                     /locus_tag="VIS73_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY5763384.1"
                     /translation="MPLFSMLPDSELDRIAQLANSRTVPRGTLLVRQGDPTDALFILI
                     SGSIKVYNCDDEGREVILSILGPGEFFGEMGLIDGAPRSANVLARESCDLIVLGKDAF
                     RRCLDEQSDVAWQMMRSLVGRLREADRKIESLALMDVYGRVAKLLLEQSEPVDGRRVV
                     TRKLPKQDIAKMVGASREMVSRVMKGLESSGYISIEEAGIVLLEH"
     gene            3066..5384
                     /locus_tag="VIS73_09275"
     CDS             3066..5384
                     /locus_tag="VIS73_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY5763385.1"
                     /translation="MPPRSRNASVALANSQTSALPDRLAAILQESRWLILAAVALFLS
                     LSLAGFDRADPGWSHAVQVDEVANPAGRFGAWLADLSLFLFGHSAWWLVALLLVLVWS
                     GYRRLHAASTPTSRRALFAVLGGFVLLLVSSCALEALRFHSHGAELPFESGGMLGTEL
                     ALLTLRYLGFTGGTLVLITLMAAGSAVFFGFSWLRLAERLGTLAELGYFAARDAFERW
                     NDRRIGEQVAKKREAVVEVEKKKVEMHEPIFIEMPETEVKVSKKAEKRIERERQVPLF
                     PDAVDGGLMPPLHLLDAAAPVTDLPSAETLEFTSRLIERKLGDFGVEVKVVAAYPGPV
                     ITRYEIEPATGVKGAVVVNLAKDLARALSLVSIRVVETVPGKSCMALELPNPKRQTVR
                     LSEIISSRAYHDQKSALTLTLGKDIGGHPVVADLAKMPHLLVAGTTGSGKSVGINAMI
                     LSLIYKSEPDLVRLILVDPKMLELSVYEGIPHLLAPVVTDMRQAANALSWCVGEMERR
                     YKLMSATGVRNLAGFNSRVVEAKKNGSPLKNPLSLTPETPEALETMPYIVVVIDELAD
                     LMMVVGKKVEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRISFQVSSK
                     IDSRTILDQMGAEALLGQGDMLHLAPGTGYPTRVHGAFVADDEVHRVVDYLKRAGPPE
                     YVEEVLAGEETEGEGGEGGGADDAESDALYDQAVEIVLKSRRASISLVQRNLRIGYNR
                     AARMIEAMEKSGLVSAMDSRGGREVLAPVRSE"
BASE COUNT          881 a         1736 c         1863 g         1017 t
ORIGIN      
        1 ccaccgcggc ggattctcgc aatgatccac aagaacgagg cgcgcctgtg ttcggtcggg
       61 tggttccgca ccgctgagcg cgagaacttc gcgcgctatt cgacaccgat ccatgttgac
      121 cggccgcaca tcgttctcgc caattccgac gcatgggccg cagtcagcgc caagggcgcg
      181 ctgctgacgc tgatggacga cccggacctg cggctcggcc tggtcgacgg ctactcctat
      241 ggcacctatc tcgacgcgct gatcgccgcc cggcaggggc cgaccaacac cgattcgctc
      301 gacgcgtcac agctgctgcg aatgctcgcg gtccgccgcg tcgatttcgc gctgttcgac
      361 cgtgaggaag ccgacgcgat ggtcaccggc aacgagtccg ccaaggcttt gcgcaccgtt
      421 ttcctgaacg gcatgccgga gggacagcgg cgccacctga tctgcagcct gcaggtcagc
      481 gaggccgaaa tcaaggcgat caacgcggtg atcgcgcgcg agccgcgcac ggtgcggcgc
      541 tgagccgact cagtagccgc gcaacagcac ctgaattgcg ccttcgccgc cgtcctgctg
      601 ccgtgcctgg cagtaggcga gcacgccggc tcggccgagc agccactggc ggctgagcag
      661 cttaagaatg gagaccccgc ccggcgagcc gagcccgcgt ccgtgaatga tgcggaccac
      721 gcgccggcgc tgcgccaggc agtcatcgag gaaggcgtcg atggcctgtc gcgcttcatt
      781 gcgcttcatg ccgtggaggt cgaggaccgc ttcggcaatc cagcgcccac gccgcagatc
      841 ggcgagcgtt cgtcgcgaga cgccgggctg ggcgaaggat tcctcggcgc cgccttcaag
      901 cagcaatggg ccatcgggtt ccgcgaggct ttcgcgcaag gccagctgct cgtcggcctc
      961 gcgctgtcgc gggagtggag ccgggcgcgg tttctggatt cgcgcccggt tgcagggcgg
     1021 cagcggcttg acgtcggcaa gcgccgcggc gagcagcgcc tgatcgtcgc cgccgtcgct
     1081 tggcggtccg gggtcgggca gttcggcgcc gggcgcggtg tgctttcggc gcagcgccgc
     1141 tttcagcggc gcccgaaaat agcgcgccgc catcgtcctc agctgcccag cgcgtcgagg
     1201 tagcgctcgg cgtcgagcgc cgccatgcaa ccggttcccg ccgaagtgac ggcttggcga
     1261 tagatgtggt cttgcacgtc accggcggcg aatacgccgg caacgctggt ctgcgtggcg
     1321 ttgccgttgc ggccgcactc ggtgatcagg tagccgccgt ccatctcaag ctggccgacg
     1381 aagagatcgg tgttcggctt gtggccgatg gcgatgaaga cacccatcag cttgaggtct
     1441 tctgtcttgc cgtccttcgt gcccttaatg cgcatgccgg tgacaccgct gtcgtcgccg
     1501 agcacttcgt cgagggtcgc gtcgagcttg aggctgatct tgccttcctt gaccttggcc
     1561 atcaggtggt cgatcaggat tttctcggcg cggaaggtgt cgcgtcgatg gaccagcgtc
     1621 acatgcttgg cgatgttgga gaggtagagc gcttcctcga ccgccgtatt gccgccgccg
     1681 atgaccgcga catcctgatt gcgatagaag aagccgtcgc aggtggcaca ggcagaaacg
     1741 cccttgccca tgaacttctc ttcggacggc aggccgaggt actgcgccga agcgccggtg
     1801 gcgatgatca gcgcgtcgca ggtgtaggtg cccgagtcgc cgatcagcgt gaacggccgc
     1861 gcccccactt tggcggtatg gatgtggtcg aagatgacct cggtctcaaa acgccgcgcg
     1921 tgctcctcga agcgcgccat cagttctggt ccctgcacgc cctgcgcgtc ggccggccag
     1981 ttgtcgacgt cggtggtggt catcagttgc ccgccctgcg cgaggccggt gatcagcacc
     2041 ggcttgaggt tggcgcgggc ggcgtagacg gcggcggtgt agccggcggg gccggaaccg
     2101 aggatcagca gtcggcagtg acggtttgct tgggtggcca tggcagtctc gatggtgggt
     2161 tggcgagttc gcgaaggcgc gaattatacc ggcggtgatt gcgtgcctca ggcggctgcg
     2221 cgatacgccc cacttgcgcg aaagcgcacc gcaatggcat ggtgacggcc aagcgttggc
     2281 gcgtataatc ctgccgaaaa tcggagtcaa gtacagatgg ctgaaaaatg tagcgcaatt
     2341 cgcgtatcgg caacgacact tcgttcgatg ccgcttttct cgatgcttcc ggattctgag
     2401 ctcgaccgga tagcgcagct cgccaacagc cgcaccgttc cgcgcggcac gctgctcgtc
     2461 cgccagggcg atccgaccga cgcgctgttc atcctgatct cgggcagcat caaggtctac
     2521 aactgcgacg acgagggacg cgaggtcatc ctcagcatcc tcggtccggg agagttcttc
     2581 ggcgagatgg gtctgatcga cggcgcgccg cgttcggcca acgtcctcgc ccgcgaatcc
     2641 tgtgacctca tcgtgcttgg caaggacgcc tttcgccgct gcctcgacga acaatccgat
     2701 gtcgcctggc agatgatgcg cagcctcgtc ggtcgtctgc gagaggccga tcgcaagatt
     2761 gagagtctgg cgctgatgga tgtttatggc cgtgtcgcca agctcttgct cgagcagtcg
     2821 gaaccggtcg acggacggcg ggtggtgacg cgcaagctgc cgaagcagga tatcgccaag
     2881 atggtcggcg cctcgcgcga gatggtcagc cgcgtgatga aggggcttga gtccagcggc
     2941 tacatctcga tcgaggaagc cggcatcgtc ttgctcgagc attgaccgcc gcgacggtcg
     3001 acgtcgcggc tgcgccgggc acgccgcccc tataatggcg ccttcttccg caacggattt
     3061 tcttcatgcc gcctcgttcg cgtaacgcga gtgtcgcgtt ggcgaattcg cagacctcgg
     3121 ccctgccgga ccggctggcc gcgattctcc aggagagccg ctggctgata ctggctgccg
     3181 tggcgctgtt cctcagcctc tcgctcgccg gtttcgaccg cgctgatccg ggttggtcgc
     3241 acgccgtcca ggtcgatgaa gtcgccaacc cggccggccg tttcggcgcc tggctcgccg
     3301 atctctcgct gttcctgttt ggacattcag cttggtggct ggtcgccctg ctgctggtgc
     3361 tcgtgtggtc cggctaccgg cgcctgcatg cggcgtcgac gccgaccagc cggcgcgcgc
     3421 tgtttgccgt gctcggcggc ttcgttctgc tgctggtttc cagctgcgcg ctcgaagctt
     3481 tgcgttttca cagccacggc gccgaactgc cgttcgagtc gggtggcatg ctcggcaccg
     3541 agctagcgct gctgacactg cgctacctcg gttttaccgg tggcacgctg gtcctgatta
     3601 cgctgatggc ggcggggtcg gcggtgttct tcggattctc ctggctgcgc ctcgccgaac
     3661 gtctggggac gctggctgaa ctcggctatt ttgccgctcg cgatgccttt gaacgctgga
     3721 acgaccgccg catcggcgag caggtcgcga agaagcgtga agcggtggtc gaggtcgaga
     3781 agaagaaggt cgagatgcat gagccgatct tcatcgagat gcccgagacc gaggtcaagg
     3841 tgtcgaagaa ggccgagaaa cgcatcgagc gagagcggca ggtgccgctg tttccggacg
     3901 cagtcgatgg cggcctgatg ccgccgctgc acttgctcga tgccgcggcg ccggtcaccg
     3961 atctgccgtc ggccgagacg ctcgaattca cttcccgcct gatcgaacgg aagctcggtg
     4021 acttcggcgt ggaggtcaag gtagtcgcgg catatccggg cccggtgatc acccgctacg
     4081 agatcgagcc ggccaccggc gtcaagggtg ccgttgtcgt caatctggcc aaggacctgg
     4141 cgcgggcgct gtcgctggtg tcgatccgcg tcgtcgagac ggtgccgggc aagtcgtgca
     4201 tggcgcttga gctgcccaat cccaagaggc agaccgtgcg cctctcggaa atcatcagtt
     4261 cgcgcgccta ccacgaccag aaaagcgcgc tgacgctgac gttgggcaag gacattggcg
     4321 gccatccggt ggttgccgat ctcgccaaga tgccgcactt gctggtcgcc ggcaccaccg
     4381 gatctggcaa gtcggtcggc atcaacgcga tgatcctgtc gctgatctac aagtccgaac
     4441 ccgatctagt gcggttgata ctggtcgatc cgaagatgct tgagctgtcg gtctacgagg
     4501 gcatcccgca tctgctggcg ccggttgtca ccgacatgcg gcaggcggcc aacgcgctgt
     4561 cgtggtgcgt tggcgagatg gagcgtcgct acaagctgat gagcgcaacc ggcgtgcgca
     4621 acctagccgg cttcaacagc cgcgtcgtcg aggcgaagaa gaacggcagc ccgctgaaga
     4681 acccgctttc gctgacgcct gagacgccgg aggcgctcga gaccatgccc tacatcgtcg
     4741 tcgtcattga cgaactggcc gacctgatga tggtggtcgg caagaaggtc gaggagttga
     4801 tcgcgcgtct ggcgcaaaag gcgcgcgccg ccggcatcca cctgattctg gcgacgcagc
     4861 ggccgtcggt cgacgtgatt accggcctga tcaaggccaa tatcccgacg cggatttcct
     4921 tccaggtgtc ttcgaagatc gactcgcgga ccattctcga ccagatgggc gccgaggcgc
     4981 tgcttgggca gggcgacatg ctgcatctgg cgccggggac aggttatccg acccgcgtgc
     5041 acggcgcttt cgtcgccgac gacgaggtcc atcgcgtcgt cgattacctt aagcgcgccg
     5101 ggccgcccga gtacgtcgag gaagtcctcg ccggcgagga aaccgagggc gaaggcggag
     5161 agggcggtgg cgcagacgat gccgagtccg atgccctcta cgaccaggcc gttgagatcg
     5221 tgcttaagtc gcgccgtgcc tcgatatcgc tggtgcaacg caatctgcgc atcggctaca
     5281 accgggccgc gcgcatgatc gaggcgatgg aaaagtcggg gctcgtatcg gcgatggatt
     5341 cgcgcggtgg ccgcgaagtg ctggcgccgg tgcgcagcga atgaagcggc ttctcctcgc
     5401 cgccgcgctg gtgttgctgg ccagcgttcc cgcgcgtgcc gacaacgtcg cgctgttgcg
     5461 cgatttcatg gcgatgacga cctcgctgca ggcagaa
//
LOCUS       DATDAA010000300         2275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_372251,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000300 DATDAA010000000
VERSION     DATDAA010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372251"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..974)
                     /gene="folD"
                     /locus_tag="VIS73_09280"
     CDS             complement(114..974)
                     /gene="folD"
                     /locus_tag="VIS73_09280"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEY5763386.1"
                     /translation="MAARLLDGKQIAAEVRAAVAARVAERTASGRRVPGLAVIQVGDD
                     PASRVYVGSKHRACAEVGLHSEAHELPVETGQAELLALIDRCNADPALDGILVQLPLP
                     AHIDADAVIERIDPAKDVDGFHPYNIGRLALRNPQLRPCTPRGIMTLLERTGIELAGR
                     DAVVVGASNIVGRPMMLELLHARCTVTVCHSRTTDLHGHVGRAEVLVVAVGKPAFVPG
                     DWVRDGAVVIDVGINRLPDGRLTGDVGFEAACERAAWITPVPGGVGPMTVATLIENTA
                     DAADARDAAA"
     gene            1130..1206
                     /locus_tag="VIS73_09285"
     tRNA            1130..1206
                     /locus_tag="VIS73_09285"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1164..1166,aa:Pro,seq:tgg)
     gene            1240..1316
                     /locus_tag="VIS73_09290"
     tRNA            1240..1316
                     /locus_tag="VIS73_09290"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1274..1276,aa:Arg,seq:tct)
     gene            1356..1431
                     /locus_tag="VIS73_09295"
     tRNA            1356..1431
                     /locus_tag="VIS73_09295"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1389..1391,aa:His,seq:gtg)
     gene            1445..1520
                     /locus_tag="VIS73_09300"
     tRNA            1445..1520
                     /locus_tag="VIS73_09300"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1478..1480,aa:Lys,seq:ctt)
     gene            1738..2124
                     /locus_tag="VIS73_09305"
     CDS             1738..2124
                     /locus_tag="VIS73_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763387.1"
                     /translation="MFDPITIPYDCVMLFNVVKLKEGVDIEEIELALGEMCNVVKNTY
                     GDEEGGFIAGQCFRYAGFVSEEGSIGSSKTTEDHLAIVTYWKSFDQHERSHADHAFNE
                     KFSMLEQYCEETYEIGYELMWQGALE"
BASE COUNT          376 a          712 c          752 g          435 t
ORIGIN      
        1 tgatcgcggc ggggcgccgc tcctacaatc catgttcccc tgtaggaggg ccgcccccgg
       61 cccgacaagc gcctggatgg gtgacgggga gcggtatccc cgcttttcag acttcaggcc
      121 gccgcgtcgc gcgcgtccgc cgcgtcggcg gtgttctcga tcagcgtcgc caccgtcatc
      181 ggcccgaccc cgccgggtac cggcgtgatc caggcggcgc gctcgcacgc agcctcgaac
      241 ccgacgtcgc cggtcagccg cccgtcgggc agccggttga tgccgacgtc gatcaccacg
      301 gccccgtcgc gcacccagtc gccgggcacg aaggccggct tgccgaccgc gaccaccagc
      361 acctcggcgc ggcccacgtg gccgtggaga tcagtggtgc gcgagtggca cacggtcacg
      421 gtgcagcgcg cgtgtagcag ttccagcatc atcggccgtc cgacgatgtt ggaggcgccg
      481 accaccaccg cgtcgcgccc ggccagctcg atgccggtgc gctcgagcag ggtcatgatg
      541 ccgcgcggcg tgcacggtcg cagctgcggg ttgcgcagcg cgaggcggcc gatgttgtag
      601 gggtggaagc cgtcgacgtc cttggccggg tcgatgcgtt cgatcaccgc gtcggcgtcg
      661 atgtgggccg gcagcgggag ttgcacgagg atgccgtcga gcgccgggtc ggcgttgcag
      721 cggtcgatca gcgcgagcag ctcggcctgt ccggtctcca cgggcagctc gtgcgcctcg
      781 gagtggaggc cgacctcggc gcaggcgcgg tgcttgctgc cgacgtagac gcgcgaggcg
      841 gggtcgtcgc cgacctggat cacggccagg cccggcacgc ggcggccgga ggcggtgcgt
      901 tcggccaccc gcgcggcgac ggcggcgcgc acctcggccg cgatctgctt gccgtcgagc
      961 aaccgtgcgg ccatggctca tccccctcgg aaaacctgat tttcccacgg ccctgcgaag
     1021 gcgaacaagg cgcgttccga ctcgattgac cccccgccga gggcgcgggt atcatgcgcg
     1081 atctgcgtgc ggcgaccgtg ttactgcgtg tgctatgatc ccgcgccatc ggggtatagc
     1141 gcagcctggt agcgcacctg ctttgggagc agggtgtcgg gggttcaaat ccctctaccc
     1201 cgaccagacg cagctgcgcc agcggaccga catcgacatg cgcccgtagc tcagctggat
     1261 agagcatcgg ccttctaagc cgagggtcgc aggttcgagt cctgccgggc gcgccaaatg
     1321 atgcggcccc cgggtcgcgg cggttggaat caatggtggg cgtagctcag ttggtagagc
     1381 cccggattgt gattccggtg gtcgtgggtt cgatccccat cgctcacccc atagatgtaa
     1441 atttgggctg ttagctcagt tggtagagca gctgactctt aatcagtagg tccggggttc
     1501 gaatccctga cagcccacca aaaaaacaaa ggcttatcga gaaatcgata ggcctttttc
     1561 tttggtttct ggatttgggt tcccctatgg gttcccttgt catcgctgcc tgattattac
     1621 cgcgcctggt ggatagcaac tccatccgaa cggtcttcac gggagtgccc aataatttcc
     1681 ctaaggtttg actacgctag gggggctcgc caccgagtgt tgacgttgga ggtcgccatg
     1741 tttgacccga tcaccattcc ctacgattgc gtcatgctgt tcaacgtcgt caagctcaag
     1801 gaaggggtcg acatcgagga aatcgaattg gccctcggag agatgtgcaa cgtcgtcaag
     1861 aatacctatg gcgacgagga gggcggattc attgccgggc agtgtttccg ctatgccggc
     1921 tttgtctcgg aggaaggatc aatcggctct tcgaagacca cggaagatca cctcgcgatc
     1981 gtcacctact ggaaatcgtt cgatcagcac gagcgttcac atgccgatca cgccttcaat
     2041 gagaagttca gcatgctcga gcagtactgc gaggagacct atgagatcgg ctacgaactg
     2101 atgtggcagg gcgcactcga gtgaggcgct tgctcgccgc ctagcttttt tgcacaggtg
     2161 ctgctgggtt cgaatgcctg acagcccacc aatatcaagg cctgccgggc gatcggcagg
     2221 ccttttttcg ttgttcggcg tcgtgagcga tcacactctt gttgaccgtg ggcat
//
LOCUS       DATDAA010000301         2442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_372369,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000301 DATDAA010000000
VERSION     DATDAA010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372369"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VIS73_09310"
     CDS             <1..681
                     /locus_tag="VIS73_09310"
                     /inference="COORDINATES: protein motif:HMM:NF028495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HEY5763388.1"
                     /translation="DWEIAAAGEGSASEQWRLRASDAEFALELSLDALKPPVLNGIDG
                     LSQKSSEPGNASYYYSITRWQAQGNVQIGGDDYTVSGLAWLDREWSSSALGADQQGWD
                     WFALQLADGSELMFYNLRKADGSQDEHSAGTWVSPDGTARHLDRDEVSIEADDTWPSP
                     EGGAYPSAWTIELPGYGLSLKVAPVLDDQELFTTVRYWEGAVDVTGTREGASVDGRGY
                     VELTGYAD"
     gene            complement(708..1505)
                     /locus_tag="VIS73_09315"
     CDS             complement(708..1505)
                     /locus_tag="VIS73_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY5763389.1"
                     /translation="MHRRLLLLALLAPVALAAAEETAWPGGIAFIELGPADGAPPVVE
                     YDGKRVLVMQDGDRWRAAVGIPLEAPMGRATIRVAGDITLSFDVVEHVYREQRLNVSK
                     SYVSLSDENLARVGRERKIIDAALTNWRETKLDGVDLAAPVDGPQSSSFGLRRFFNDE
                     PRAPHKGMDIAAAEGTAVLAPRDGVVTATGDYYFNGNTVIIDHGQGYVTMYCHLSEIR
                     VDEGQDLAAGDLIGAVGATGRVTGPHLHFGTYLNGTAVDPALLLTRE"
     gene            complement(1498..>2442)
                     /gene="xseA"
                     /locus_tag="VIS73_09320"
     CDS             complement(1498..>2442)
                     /gene="xseA"
                     /locus_tag="VIS73_09320"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY5763390.1"
                     /translation="QFELLKNKLAAEGLFDEAHKQELPALPARIGVITSPSGAAIRDI
                     ISVLRRRFPSIPVVIYPAAVQGEAAPAEIIAALETAIRRDECDVLIMGRGGGSLEDLW
                     AFNDEKLARAIFDSPIPIVSAVGHEIDFTIADFVADVRAPTPSGAAEIVVPDRLDWLR
                     AVNSLTARVARLGQRALEDKGQTLDWLSRRLLQGSPQSTLRRQEAQLREVRHRLAAAM
                     RQSALWLRPQLTSLSQRLSSSGRNRIAAASHRLDLAMRGLHSVSPLATLDRGYAIVER
                     ADTGKVVLNAADVAAGDDIRARLAQGELIATVKSTKNA"
BASE COUNT          434 a          770 c          804 g          434 t
ORIGIN      
        1 gactgggaaa ttgctgcggc gggcgagggc agtgcttccg aacaatggcg gctgcgcgca
       61 agcgacgccg agtttgcgct cgagctgtcg cttgatgcgc tgaaaccgcc ggtcctgaac
      121 ggtattgacg gtctgtccca aaaatcgtcc gaacccggca acgcctcgta ttactactcg
      181 atcacgcgtt ggcaggccca gggcaacgtg cagatcggcg gcgacgacta cacggtgtcg
      241 ggtctcgcct ggctggatcg ggagtggagc agcagcgcgc tcggcgccga ccaacagggc
      301 tgggactggt ttgcgctgca gctcgccgac ggcagcgagc tgatgttcta caacctgcga
      361 aaagccgacg gctcgcagga cgaacacagc gccggcacct gggtctcgcc ggatggcaca
      421 gcgcggcacc tggatcgcga tgaagtttcg atcgaagctg atgatacctg gccgagtccc
      481 gaaggcggcg cctatccgtc tgcctggacg atcgagctgc cgggctacgg gctgagtctg
      541 aaagttgccc ctgtcctgga cgaccaggaa ttattcacaa cggtgcggta ttgggaaggt
      601 gccgtggatg tgacgggtac gcgcgaaggc gcctctgtcg atgggcgcgg ttatgtcgaa
      661 ttgaccggtt acgcggacta ggtgactttc gacctcgggc gaagacttta ttctcgagtc
      721 aacaaaagag cgggatcgac ggcagtgccg ttcaggtagg tgccgaagtg caggtgcggg
      781 cccgtgacgc gccccgtcgc gccgaccgca ccgattaaat caccagcggc caggtcctgt
      841 ccctcgtcaa cacggatttc gctgaggtgg cagtacatcg tgacatagcc ctgaccgtga
      901 tcgatgatca cggtattgcc gttgaagtaa taatcgccgg tcgcggtcac gacgccgtct
      961 ctaggcgcca gcaccgccgt accctcggct gcggcgatat ccatgccttt gtggggcgcg
     1021 cgcggttcgt cattgaagaa ccggcgcagg ccgaagctcg aactttgcgg accatcgacg
     1081 ggggcggcca ggtctacgcc atcgagcttg gtctcacgcc agttcgttaa agccgcatcg
     1141 atgattttgc gttcgcgtcc gacgcgagcg agattttcgt cgctcagcga cacgtagctc
     1201 ttactgacgt tcagccgttg ttcgcggtag acatgctcga cgacgtcgaa agacagcgtg
     1261 atgtcgccgg caacccgaat cgttgcgcgc cccatgggcg cctccagggg tatgccgacc
     1321 gccgcgcgcc agcgatcgcc atcctgcatc acgagcaccc gcttgccgtc gtactcgacg
     1381 accggcggtg ccccgtcggc agggccgagc tcgataaatg caatgccgcc gggccacgcc
     1441 gtctcctctg ctgccgcaag cgcaaccggc gcgagcaacg ccaggagcag caggcgccta
     1501 tgcatttttc gtagacttca cggtcgcgat cagttcgcct tgcgccaggc gcgcgcgtat
     1561 atcgtccccg gcggcgacgt cggcggcatt cagcacaacc ttccctgtgt ctgcgcgttc
     1621 gacgatcgcg tagccccgat ccagcgttgc gaggggactg accgaatgca ggccgcgcat
     1681 ggcgaggtcc agccggtgac tcgcagccgc aatccggttg cgcccactgc tgctcagccg
     1741 ttgagacagc gaggtcagct gcgggcggag ccacagggcg ctctgacgca tggcggcggc
     1801 gagacgatga cgtacctctc tcagctgggc ctcctggcgc cgcagtgtcg attgcgggct
     1861 accctgcagc agccggcggc tcagccagtc cagcgtctgg cccttgtcct cgagggcgcg
     1921 ctgtccgagc cgggcgacgc gcgcggtgag ggagttgacc gcgcgcagcc agtcgagccg
     1981 gtccggcacc acgatctcgg cggcgccgga gggggttggg gcgcgaacgt cagcaacgaa
     2041 gtctgcgatc gtaaaatcga tctcgtggcc tacagcgctg acaatcggta tcggcgagtc
     2101 gaagatcgcc cgcgccagtt tctcgtcatt gaatgcccag aggtcctcga gcgagccgcc
     2161 gccgcgtccc atgatcagca cgtcacattc gtcgcgccga atcgccgtct caagcgcagc
     2221 gataatttcg gccggcgcgg cttcaccctg cacggcagcc gggtagatca cgacggggat
     2281 ggaggggaag cgacgtcgaa gcacgctgat aatgtcgcga atcgcggccc cgctcggcga
     2341 cgtgatgacc ccgatgcggg ccggcagagc aggcaattcc tgcttgtggg cttcgtcgaa
     2401 cagcccctcg gcggcgagtt tgttcttcaa taactcgaac tg
//
LOCUS       DATDAA010000302         3917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_372614,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000302 DATDAA010000000
VERSION     DATDAA010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372614"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VIS73_09325"
     CDS             <1..406
                     /locus_tag="VIS73_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763391.1"
                     /translation="RRKAADVFWAFMYWYFFGGGAARIIAGALTPAAVEELGDRIEIV
                     VEDPARAAAAQMTLKNLRKEAKAFEKTFTASGKAVKRSYRDHAADLAEIEAVLDQLNR
                     DWERSQERALDLRFELRERLTREEWAALHSRD"
     gene            469..1062
                     /locus_tag="VIS73_09330"
     CDS             469..1062
                     /locus_tag="VIS73_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763392.1"
                     /translation="MLRFVTAGLAVVVSLTLAAPDTHAQRAGQSATVRTGTVTAMKSV
                     DLNDGNAIKGALVGGAFGAALTRSSKGSSRRDRNAAIGAVLGGAAAASKTRPGRIYTV
                     TTNEGTMIQIATEQTEIRVDDCVFVEEAGGSANIRRAPATACDPASQEVMKDAAIIEE
                     MQEEASECAAAKQELVDADSDDAIDRAVRKVKLLCYD"
     gene            complement(1074..2057)
                     /locus_tag="VIS73_09335"
     CDS             complement(1074..2057)
                     /locus_tag="VIS73_09335"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF024801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY5763393.1"
                     /translation="MGLLVDGEWRDQWYDTKSTGGRFKRDISRFRNWVTADGRAGPSG
                     VSGFQAEAGRYHLYVSYACPWAHRTLIFRKIKGLEDFISVSVVHWYMAENGWTFADGD
                     GVVADPIHDADYLYQVYQAADSSYTGRVTVPVLWDTTKDTIVSNESADIIRMFNSAFD
                     TIGATEGDCYPEPLREEIDEVNARVYDTVNNGVYKAGFATTQEAYEEAVEPLFESLEW
                     LDGRLSKRRYLMGDRITEADWRLFTTLLRFDPVYVGHFKCNRRRIVDFENLWPYVRDL
                     YQVPGVADTVHMDHIKSHYYASHDTINPTRIVPVGPEIDYSEAHGRDALSG"
     gene            complement(2089..2706)
                     /locus_tag="VIS73_09340"
     CDS             complement(2089..2706)
                     /locus_tag="VIS73_09340"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006822499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEY5763394.1"
                     /translation="MTIYTLPKLDYDYKDLEPHLSSEILELHHGKHHAAYVDGANATL
                     DNLAEARESGDYGALNKLEKDLAFHLSGHVLHSMLWKNMSPNGGGQPDGNLAAAIKES
                     FASFDAMQSQLSAAAMGIQGSGWASLAWDPLSGSLVVQQVYDHQGNVGSATIPLLVLD
                     MWEHAFYLQYRNVKAKWVDAFWNLVNWDDVEKRLAGARKTDLVLD"
     gene            2953..>3917
                     /locus_tag="VIS73_09345"
     CDS             2953..>3917
                     /locus_tag="VIS73_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HEY5763395.1"
                     /translation="MADVLAFDPGYDPRIIGLGIALAIGFVIGLEREWAEDKPVGVRS
                     FALIAAFGGLCALLLPETGGWLIATGLLALGAILILHVYRRKLEGITTLVAAFIVYLL
                     GAAAVAGYWLPAIVLGGAVMVLLHWKKPLHSWVLRLGDRDFEIIVRFVLIALVILPVL
                     PDQDLGPYSAFNPFTAWLMVVLIVGINLVGFVAFRLVGTRAGGWLAGLLGGLVSSTAT
                     TISYAGLSKRERGLGPVAALVILVASAVVYGRVLVELAVVAPDLLESMAGPAATFTAI
                     LLVLSVLVFRAVRSNTDSELPERENPARIRQALVFAALYVVILFGV"
BASE COUNT          684 a         1172 c         1272 g          789 t
ORIGIN      
        1 gcggcgcaag gcggctgacg tgttctgggc gttcatgtac tggtatttct tcggcggcgg
       61 cgccgcacgc attatcgccg gtgcgctcac gcccgcggcg gtcgaagagc tgggcgaccg
      121 tatcgagatc gtggtcgagg acccggcgcg cgccgcggcc gcgcagatga cgctgaaaaa
      181 tctcaggaag gaggctaagg ccttcgagaa gactttcacg gcatccggca aagcggtgaa
      241 gcgttcgtac cgcgaccacg cggctgatct cgccgagata gaggccgtgc tcgatcagct
      301 gaaccgagac tgggagcgga gccaggagcg ggcgctcgac ctgcgcttcg aactgcgcga
      361 gcggttgacg agagaggagt gggcggccct gcattcgaga gattagtccc ggcacttcag
      421 gtcggggcat aattggccaa gatttcaccg atatccaggg agaagaacat gcttcgcttt
      481 gtaaccgccg gtcttgccgt cgtcgtgagc ctgacgctgg ccgcgcccga tactcatgcc
      541 caacgcgccg gccagtcggc caccgtgcga acgggtaccg tcacggcgat gaaatccgtg
      601 gatctgaatg acggcaacgc gatcaagggc gcgctggtcg gtggcgcttt cggcgccgcg
      661 ctgacgcgat ccagcaaagg ctcgagccgc agggatcgca acgcggcgat cggcgccgtt
      721 ctgggcggag cggccgcagc ctccaagaca cggccgggtc gcatctacac ggtgaccacc
      781 aacgaaggca cgatgatcca gatcgcgact gagcagaccg agatccgggt cgacgactgc
      841 gtgttcgtcg aggaagccgg cggcagtgcc aacattcgcc gggcgccggc cacggcctgc
      901 gatcccgcat cgcaggaggt gatgaaagat gcggcgatca tcgaggagat gcaggaagag
      961 gcgagcgagt gcgctgcggc caagcaggaa ctcgtcgatg ccgacagcga cgatgcgatc
     1021 gaccgcgccg ttcgtaaggt gaaactgctc tgttacgact agccgacgcg atctcaaccc
     1081 gatagtgcgt cacggccgtg cgcctccgaa tagtcgattt cggggccgac cggcacgatg
     1141 cgcgtcgggt tgattgtgtc gtggctggcg taatagtgcg acttgatgtg gtccatgtgc
     1201 accgtgtcgg ccacgccggg gacctggtac aggtctcgca catagggcca caggttctcg
     1261 aaatccacaa tgcgtcggcg gttgcacttg aagtggccga cgtataccgg gtcgaagcgc
     1321 agtagcgtcg tgaacaggcg ccagtccgcc tcggtgattc ggtcgcccat caggtagcgc
     1381 cgcttcgaca ggcggccgtc gagccattcg agtgattcga acagcggctc gacggcttct
     1441 tcgtaggctt cctgcgtggt cgcgaaaccc gccttgtaaa cgccgttgtt gacggtgtcg
     1501 tagacgcggg cgttgacttc atcgatctcc tcgcgcagcg gctcgggata gcaatcgcct
     1561 tcggtcgctc cgatcgtgtc gaacgccgag ttgaacatgc ggatgatatc ggcggactcg
     1621 ttgctgacga tcgtgtcctt cgtcgtgtcc caaaggaccg gaaccgtcac ccggccggta
     1681 taggacgagt cggctgcctg gtagacctgg tacaggtagt cggcatcatg gatcggatcg
     1741 gcgaccacgc cgtcgccatc cgcaaacgtc cagccgttct cggccatgta ccagtggacg
     1801 acggagacgg agataaagtc ttcgaggcct ttgatcttgc ggaatatgag ggtgcggtgc
     1861 gcccaggggc aggcatagga aacgtacagg tgataccggc ctgcctccgc ctggaaaccg
     1921 gacacgccgc tgggtcccgc gcggccatcg gcggtgaccc agttacgaaa ccgcgatatg
     1981 tcgcgcttga agcggcctcc cgtggacttg gtgtcgtacc attggtcgcg ccattctcca
     2041 tcaaccagca aacccattcc ctcaactcct tgttggcgac gtgttcgttc agtccagtac
     2101 gaggtcggtt ttcctggcgc ccgcgaggcg cttctcgacg tcatcccagt ttacgaggtt
     2161 ccagaatgcg tcgacccact tggccttcac gttgcggtac tgcaggtaga aggcgtgttc
     2221 ccacatgtcc aggacgagca gcgggatggt tgcgctgccg acgttgccct gatggtcgta
     2281 aacctgctgg acgaccaggc tgccggacag cgggtcccat gcgagactgg cccatccgga
     2341 gccctggatg cccatagcgg ccgccgacag ctggctctgc atggcgtcga atgaggcgaa
     2401 cgattccttg atggccgcag cgaggttgcc gtccggttgg ccgcccccgt tggggctcat
     2461 attcttccaa agcatggaat gcagaacgtg gccggatagg tggaaagcaa gatccttctc
     2521 gagcttgttc aatgcgccgt aatcgcccga ttcacgcgcc tcggcgagat tatcgagggt
     2581 cgcgtttgcg ccatccacgt acgccgcgtg gtgcttgccg tggtgcagtt ccaggatttc
     2641 cgaggaaaga tgtggctcca ggtccttgta gtcgtagtcc agtttcggaa gcgtgtagat
     2701 agtcacagtt cagtcccttt tcataatcga aagaaaaaca gccggcgatt gattcgccgg
     2761 cactggaatg ctgctcgtgt ctgtttagat ttggggctga tcggaaattt gcaaggcacg
     2821 cgccaccaac tgtctttgag agacatatgg gcacccggtg cgccttaaat tgagggaaga
     2881 tacgtagacg atgccggtgc cttatcgcgt agtctcttaa atgcgtgcga actcggcgcg
     2941 cgggagcttg atgtggctga tgtgctcgct ttcgatcccg gctacgaccc ccggatcatc
     3001 ggactgggaa tcgcgctcgc gatcggtttc gtcatcggtc ttgagcgaga atgggccgag
     3061 gacaaacccg tcggcgtgcg ttccttcgcc ctgatcgcgg catttggcgg tctatgcgcc
     3121 ctcctgctcc cggagacggg cggttggctg attgccaccg gcctgctggc gcttggcgcc
     3181 atcctcatcc tgcacgttta tcgccgcaag ctggaaggaa taaccaccct ggttgcagca
     3241 ttcatcgtgt acctgctcgg tgcggccgcc gtagccggct actggttgcc cgcgatcgtg
     3301 ctgggcggcg ccgtcatggt gctgctgcac tggaagaagc cgctgcacag ctgggttctg
     3361 cggctgggcg accgggattt cgagatcatc gtgcgcttcg tgctgatcgc gctggtgatt
     3421 cttcccgttc tgccggatca ggatctcggc ccttactcgg cattcaatcc atttacggca
     3481 tggttaatgg tcgtgctgat cgtcggcatc aacctggtgg gtttcgtggc ctttcggctc
     3541 gtcggcacac gcgccggcgg ctggctggcc gggctgctcg gtggcctcgt gtcgagtacc
     3601 gcaaccacca tcagctacgc cggcctgtcg aagcgggaac gcggtctcgg cccggttgcg
     3661 gcgctcgtca tcctcgttgc cagtgcggtc gtctacggcc gggtcctcgt cgagctggcc
     3721 gtcgtcgccc ccgacctgct cgagagcatg gccgggcccg cggccacctt caccgcgata
     3781 ttgctggtgt tgagcgtcct ggtattccgt gccgttcgca gcaacaccga tagcgaattg
     3841 ccggaacgcg agaatcccgc gcgcatccgc caggcactcg tattcgcggc gctttacgtc
     3901 gtcatcctgt tcggggt
//
LOCUS       DATDAA010000303         3710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_337245,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000303 DATDAA010000000
VERSION     DATDAA010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337245"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VIS73_09350"
     CDS             complement(<1..255)
                     /locus_tag="VIS73_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEY5763396.1"
                     /translation="MHILILGIGNTLLSDEGVGVAAMQMLADELGSDPDLEFLDGGTL
                     SFTLAVPISACDGLIVLDAAMIDGPSGSVGVYEDEAMEEFL"
     gene            complement(268..1731)
                     /gene="glgA"
                     /locus_tag="VIS73_09355"
     CDS             complement(268..1731)
                     /gene="glgA"
                     /locus_tag="VIS73_09355"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEY5763397.1"
                     /translation="MRVLHLAAEIYPLVKTGGLADVIAALPPALAARDLDVRVLLPGH
                     PAILDGLRDARTVITLGPAFGAAVIKLVGGRLEPSGISAYVIDAPFLYRREGNPYVDP
                     LGIDWTDNHRRFALLGWIAAHLASGELDAEWIPQIVHGHDWHAGLAPAYIAQNPAMTT
                     ATVFTIHNLAYRGIFGIDAAGELGLAARKLTPMGLEFHGQISFMKAGLVYSRRLTTVS
                     PTYAREICTPEYGEGLDGVLRDRGPALSGILNGVDYTVWSPETDTAIKARYSARNLGG
                     KALCKADVQTAFGLPVEAGVPLFTVVSRLSAQKGLDLLEAILPELSGAGAQLVVLGSG
                     DGAIEMQWRRAAATHPHAVAVHVGYDESISHRLIAGADALIVPSRFEPCGLTQLYALR
                     YGTVPVVRRVGGLADTVVDASPANLAAGTATGFVFGESNGDALASRLHDACALFRDKA
                     AWKRLQKTGMGQDFSWNESAAHYETLYVDMLGATTGH"
     gene            complement(1747..3030)
                     /gene="glgC"
                     /locus_tag="VIS73_09360"
     CDS             complement(1747..3030)
                     /gene="glgC"
                     /locus_tag="VIS73_09360"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY5763398.1"
                     /translation="MNFKERRTYRLPGRAIAIVLAGGRGSRLHELTDRRAKPAVYFGG
                     KFRIVDFALSNCVNSGIRRIGVVTQYKSHSLLRHLQRGWSFLRGEVNEFVDLLPAQQR
                     VDEESWYRGTADAVYQNIDIIDTYHPTPEYVVVLAGDHVYKMNYASMLVDHVEHGAEC
                     TVACIEVPRQEASGFGVMAVDETNRIVDFVEKPADPPCMPGKPDTALCSMGIYVFNAK
                     YLYAELERDIADPKSSHDFGKDIIPAAVRNGVAQAHPFGRSCVFAPEEHHKDAYWRDV
                     GTIDAYWDANIDLTSTEPHLNLYDRGWPVWTYQQQLPPAKFVHNQPGRRGVAVESTVS
                     GGCIISGELNRSLLFSICRIHSYAKVNWSVLLPEVDVGRGARLTRCIVDHGCVIPEGL
                     VVGEDPEEDARRFRRTANGITLITRQMLDRIARQK"
     gene            3287..>3710
                     /locus_tag="VIS73_09365"
     CDS             3287..>3710
                     /locus_tag="VIS73_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5763399.1"
                     /translation="MRVSATDRCDLRCGYCIPNGHAGFCERPDVLRADEFARLISLFA
                     ELGVSRVRLTGGEPLTRADLPKIAERIGAIAGIDDASLSTNATQLASQAIALHAAGVR
                     RLNVSLDSLGRQRFAAIVGRDCLDDVLRGLEAASAAGFS"
BASE COUNT          597 a         1182 c         1283 g          648 t
ORIGIN      
        1 gaggaattcc tccattgcct cgtcttcgta gacgccgacg ctgccgctcg ggccgtcgat
       61 catcgctgcg tcgagaacga tcagtccgtc gcaggccgaa atcggtaccg ctagcgtgaa
      121 ggagagcgtg ccgccgtcaa ggaattcaag atcggggtcg ctgccgagtt cgtcggccag
      181 catctgcatt gccgcgacgc cgacgccttc gtcggagagc agtgtgttgc cgatgccgag
      241 gatgaggatg tgcatgcggg gcgtggttca gtggccggtc gtggcgccga gcatgtcgac
      301 gtagagcgtt tcgtagtgcg cggctgactc gttccacgag aaatcctggc ccatgccagt
      361 cttctgcagc cttttccagg ccgccttgtc gcggaagagc gcgcaggcgt cgtgcagtcg
      421 ggaggcgagt gcgtcgccgt tcgactcacc gaacacgaat ccggtggcag tgcccgcggc
      481 gaggttggcg gggctggcgt cgaccacggt gtcggcgagg ccgccaacgc ggcgcaccac
      541 cggcaccgtg ccgtaacgca acgcgtaaag ctgggtcagc ccgcagggtt cgaaacgcga
      601 gggcacgatc agcgcgtcgg caccggcgat caaccggtgc gagatggact cgtcgtaacc
      661 gacgtggacg gcgaccgcgt gcgggtgcgt ggccgccgca cgccgccact gcatctcgat
      721 cgcgccatcg ccgctgccga gcacgaccaa ctgcgccccg gcgccggaca gttcgggcag
      781 gatcgcttcg agcaggtcga gtcccttctg tgcagacagc cggctgacca ccgtaaacag
      841 cggcaccccg gcttcaacag gcagtccgaa ggccgtttgc acgtcggcct tgcacaacgc
      901 cttgccgcca agattccggg ccgaatagcg ggccttgatc gcggtgtccg tttcggggct
      961 ccacaccgtg taatcgacgc cgttgaggat gcccgagagg gctgggccgc gatcgcgcag
     1021 cacgccgtcg aggccctcgc cgtactccgg cgtgcagatt tcccgcgcat aggtcgggct
     1081 gaccgtggtg aggcgtcgtg aatagaccag accggccttc atgaaggaga tctggccgtg
     1141 gaattcaagg cccatcggcg tcagctttcg cgccgccagg ccgagttcgc cggccgcatc
     1201 gattccgaag atgccgcgat aggcgaggtt atggatggtg aaaaccgtcg ccgtggtcat
     1261 cgccgggttc tgcgcgatat aggccggcgc caggccggcg tgccaatcgt ggccgtggac
     1321 gatctgcgga atccattctg cgtcgagctc gccgctcgcg aggtgggcgg cgatccagcc
     1381 gagcagcgcg aagcggcggt ggttgtcggt ccagtcgatg ccgagggggt ccacgtaagg
     1441 attgccctcg cggcggtaga ggaagggtgc gtcgatgacg taggcggaga tgcccgaggg
     1501 ttcgaggcgg ccgccgacca gcttgatcac cgcggcgccg aaggcgggcc ccagcgtgat
     1561 cacggtccgg gcgtcgcgca gtccgtcgag aatcgccgga tgccccggca gcagcacgcg
     1621 gacgtcgagg tcgcgggcgg ctagcgccgg gggcagggca gcgatgacgt cggcgaggcc
     1681 gccagtcttg accagcgggt agatttcggc ggcgaggtgg agcacgcgca tcgtctgccc
     1741 gctcgtctat ttctgccgcg cgatccggtc gagcatctgc cgagtgatca gcgtgatgcc
     1801 gttggccgtg cgacggaagc ggcgggcgtc ctcctcgggg tcttcgccga ccaccagccc
     1861 ctcgggaatc acgcagccgt ggtcgacgat gcagcgggtc agccgtgcgc ctcgcccgac
     1921 atcgacctcg ggcagcagca ccgaccagtt caccttggcg taggagtgga tgcggcaaat
     1981 cgaaaacagc aatgagcggt tcagttcgcc ggagatgatg cagccgccgg aaaccgtcga
     2041 ctcgactgca acgccgcggc ggcccggctg gttgtggacg aacttggccg gcggcaactg
     2101 ctgctggtag gtccacacag gccagccgcg atcatagagg ttgaggtgcg gctcggtgct
     2161 ggtgaggtcg atattggcgt cccagtaggc gtctatggtg ccgacgtcgc gccagtatgc
     2221 gtccttgtga tgttcctcgg gtgcgaacac gcagctgcgg ccgaaggggt gcgcctgcgc
     2281 cacgccgttt ctcaccgcgg ccggaatgat gtccttgccg aagtcgtggc tggatttcgg
     2341 gtcggcgata tcgcgttcga gttcggcgta caggtatttg gcgttgaaga catagatgcc
     2401 catactgcac agcgccgtat cgggcttgcc gggcatgcac ggcgggtcgg cgggtttctc
     2461 gacgaaatcg acgatgcggt tggtctcgtc gacggccatg accccgaagc cgctcgcttc
     2521 ctggcgcggt acctcgatgc aggccaccgt gcattcggcg ccgtgctcga cgtggtcgac
     2581 cagcatcgac gcgtagttca tcttgtagac gtggtcaccg gcgagcacca cgacgtactc
     2641 gggggtcggg tggtaggtgt cgatgatgtc gatgttctga tagacggcgt cggccgtgcc
     2701 gcggtaccag gattcttcgt cgacgcgctg ttgcgctggc aacaggtcga cgaactcgtt
     2761 gacctcgccg cgcaggaagc tccacccgcg ctgcaggtgg cgcagcagac tgtgcgactt
     2821 gtactgcgtg accacgccga tgcggcggat tcccgagttc acgcagttcg acaacgcgaa
     2881 atcgacgatg cggaatttgc cgccgaagta gaccgccggc ttggcccggc ggtcggtcaa
     2941 ctcatgaaga cggctgccgc gaccgcccgc gagcacgatg gcaattgccc ggccggggag
     3001 gcggtaagtc ctgcgttcct tgaagttcat gctgtcctcc actgcgcatc cgctcgttct
     3061 ccggagcggc tttcgcttca agtaaacacc aattgcgcgg catgcgcgag tttttacgga
     3121 tggatcaaat gccgctcgcg gcgcgcttga gggcccaatg gtgcgccagt acggtcggca
     3181 ggcatcggca cgccaccgga gccaacgctt gagccagccg taatcgcttc gtcagcacgg
     3241 tcaatcgacc acgctcgccg accgcttcgg gcggcgcatc agctacctgc gcgtatcggc
     3301 caccgaccgc tgcgatctgc gctgcggcta ctgcattccc aatggccacg ccggtttctg
     3361 cgagcggccc gacgtgctgc gcgccgacga gttcgcgcgg ctgatcagtc tgtttgccga
     3421 gttgggtgtg tcgcgggtgc ggctgaccgg cggcgagcca ctgacccgtg ccgacctgcc
     3481 taaaatcgcg gagcgaatcg gcgccatcgc cggcatcgac gatgcctcgc tgagcaccaa
     3541 cgccacgcag ctcgcgagcc aggcgattgc cctgcacgcc gccggcgtgc gccgcctcaa
     3601 cgtcagcctc gattcgctcg gccgccagcg cttcgccgcc atcgtcggcc gagattgcct
     3661 ggacgacgtg ttgcgcgggc ttgaagcggc cagcgccgcc ggtttctcgc
//
LOCUS       DATDAA010000304         2123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_302890,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000304 DATDAA010000000
VERSION     DATDAA010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302890"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VIS73_09370"
     CDS             <1..1294
                     /locus_tag="VIS73_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY5763400.1"
                     /translation="IDIGLERTAFLHASDIARPVTPDNGEDVPAIRELVREGDEIMVQ
                     VVKDPLGNKGARLTTYITLPSRHLVLLPRGSAVGISARIEDEEERERLRSLVGDLISE
                     QELACGVIVRTVAEGAESDALSADLRYTLKLWDVVQERCRNSSVKSLVHEDLSLPLRV
                     LRDMVTGDVERILVDSPTDFEAMQEFAATFLPDATPAIECYRRRRPIFDLHGIEEEIR
                     KSLERSIPLKSGGYLIFDQTEAMTTIDVNTGGYVGHRNLEETIYRTNLEAAVAIARQL
                     RLRNLGGIIIIDFIDMEQADHRDNVLQVLEQALARDHARHQITPLSPLGLVEMTRKRT
                     RESLQHVLCEDCPVCTGRGFVMTAETVCFEIFREIIRQSRQFEFDEVMVLANEGVIEL
                     LLDEQARSLAELAEQTGKSIRLQTEALYLQDQFDVVLM"
     gene            1302..>2123
                     /locus_tag="VIS73_09375"
     CDS             1302..>2123
                     /locus_tag="VIS73_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763401.1"
                     /translation="MKQLLRKLFSILAYTAATVVILMAVVVGLFRLFLPRLPEYQNEI
                     KAWASDAIGIGVEFSGMNARWGLSGPELEFYDGELMRPESQARLLAADRVRIGISVGS
                     LVFDRSLVVDHVFISDTSIEVRQLEGGDWWLQGSPLSELVPEGGGVAQQPTAFDLVLE
                     DVEVVFLQAGAERPREFLVPRAIASIDQNRIAVDALVRLPEDLGRQVEFSATRLVNIP
                     QEERGWDVSVEADNVDLSGWSDLHEFPGRRFLSGGGDLELSMAIAAGELVNATGQL"
BASE COUNT          383 a          627 c          697 g          416 t
ORIGIN      
        1 catcgacatc ggtctcgagc gcactgcgtt tctgcatgca tccgacattg cccgtccggt
       61 cactcccgac aatggcgaag acgtgcctgc catacgcgag ctggttcgcg agggcgacga
      121 gatcatggtg caggtcgtca aggacccgct gggtaacaag ggcgcccgcc tgacgacgta
      181 tatcacgctg ccgtcgcggc atctcgtcct gttgccgcgg ggcagcgcgg tcggcatctc
      241 cgctcgcatc gaggacgagg aagagcgcga gcgcctgcgg tcgctggtag gggatctgat
      301 tagcgagcag gaactcgctt gcggcgttat tgtgcgcacc gtcgccgagg gcgccgagag
      361 cgacgccctt tcggccgacc tgcgctatac cttgaaactc tgggacgtcg ttcaggaacg
      421 gtgcaggaac agcagtgtaa aaagcctggt acacgaggac ctgtcgctgc cgctgcgcgt
      481 gctgcgcgac atggtgaccg gggatgtcga gcgcatcctc gtcgattcac ccaccgactt
      541 cgaggccatg caggaattcg ccgcgacctt cttgccggat gcgacgccgg cgatcgaatg
      601 ctacagacgg cggcggccga tctttgacct gcacggcatc gaggaagaga tacgcaagtc
      661 gctcgagcgc agcatccctc tgaagtcggg ggggtacctg atcttcgacc agaccgaggc
      721 aatgacaacg atcgacgtca acacgggcgg ctacgtcggt catcgcaatc tcgaggagac
      781 gatctaccgc accaacctcg aggccgccgt ggcgatcgcc cgacagcttc gcctgcgtaa
      841 tctcggtggc atcatcatca tcgatttcat tgacatggag caggcagacc atcgcgacaa
      901 cgtgctgcaa gtgctcgagc aggcactggc gcgcgatcat gcccgccacc agatcacgcc
      961 gctgtcaccg cttggcctgg tcgagatgac gcgcaagcgc acgcgcgaga gtttgcagca
     1021 tgtgttgtgt gaggactgcc ccgtctgcac ggggcggggt ttcgtcatga ccgcggaaac
     1081 ggtctgtttc gagatctttc gtgagatcat tcggcagtcg aggcagttcg aattcgacga
     1141 ggtcatggtg ctggccaatg aaggcgttat agagcttctc ctcgacgagc aggcgcgcag
     1201 tctggccgag ctcgcggaac agaccggcaa gtcgattcgc ctgcagacgg aggccctgta
     1261 cctgcaggac cagttcgacg tggtcctgat gtaggtaagc gatgaagcag ctgctgcgga
     1321 aactgttttc gattctggcc tatacggccg cgacggtcgt gatcctgatg gctgtcgtcg
     1381 tcggcctgtt ccgcctgttc ctgccgcggc ttcccgaata ccagaacgag atcaaagcct
     1441 gggcgagcga tgccattggt atcggggtcg agttctctgg catgaatgcc cgctggggtc
     1501 tgagcggccc cgagctcgaa ttctatgacg gcgaactgat gcggccggaa agccaggcgc
     1561 gcctgcttgc agccgatcgc gtccgcattg gtatatccgt cgggagtctc gtgtttgatc
     1621 ggtctttagt ggttgatcac gttttcatca gtgacacctc gatcgaggta cgccagctcg
     1681 aaggcggcga ctggtggctg cagggctcac cactcagtga gctggtgcca gaaggcggag
     1741 gtgtggcgca gcagccgacc gcattcgatc tggtgcttga agatgtagag gtcgtgttcc
     1801 tgcaggccgg tgccgagcgg ccgcgcgagt tcctggtgcc acgcgcgatt gcgagcatcg
     1861 accagaaccg cattgccgtg gatgcacttg ttcgccttcc cgaggatctc gggcggcagg
     1921 tcgaattctc cgcgacgcgg ctggtgaata tcccgcagga ggagcgtggc tgggacgtca
     1981 gtgtcgaggc cgacaacgtc gatctttccg ggtggtccga cctgcacgag ttccccggcc
     2041 ggcgcttcct ctccggcggt ggcgatctgg agctgtcgat ggcgatcgcg gcgggtgaat
     2101 tggtcaatgc caccggccaa ctc
//
LOCUS       DATDAA010000305         2963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_22326,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000305 DATDAA010000000
VERSION     DATDAA010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22326"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="VIS73_09380"
     CDS             complement(<1..1634)
                     /locus_tag="VIS73_09380"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HEY5763402.1"
                     /translation="MLFDGAAAATVEQLDTLDPLPDFQDSTQQTDTQSLAEAQQVQPG
                     TAEVIESSLSDALLDPEASNGGHELLFIDPKVTDYQQLVDLAKPNVEVVVLDPLRDGL
                     QQITETLEGRSDISAIHIVSHGEAGKLYLGAGSVTLTTVVARAAELGAWSASLAPGAD
                     LLLYGCDIAAGDAGRAFVDTLSAATGADVTASTDTTGNWAYGGDWNLEYATGSIEASI
                     GLVPEVQDEWFAVLATTSFQQGVNSYAGTQDTTLWEETPDATSGANTTFDVDLDANLG
                     FESQSILRFDNIFGAGADQIPLGAIIDDVQVVLTTNGTSNTTDTINLYRMLVDWNEST
                     ATWNTLTGGLNSGVDYLATADASLTGVGDGVAANVDAIFTGANLVATLQAWSDGATSN
                     YGWAVLNSGTDGITFRSSETGTLTYRPELIVTWHLASGFANDDAASYSDEVLADSPIN
                     YFRLGEASAPLVDIGSSPATDIQLLGGVLLGQPGAIAGDSDTAVVFDGVDDAIVIGET
                     SGYATGPANGTIQFWFRTDSLTSTQGLFSRDATGTA"
     gene            complement(2141..2908)
                     /locus_tag="VIS73_09385"
     CDS             complement(2141..2908)
                     /locus_tag="VIS73_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5763403.1"
                     /translation="MLAGIQQLVLVAAKPPVGFFAYPGKPSTLWPKDCALDVLARPEE
                     DAVAALEALADELGAPREVPLPAIEAPKANVRGAFEPLAFAHAFAGVIPENAIVVEDA
                     VTSGRALFAPTFGAAPNDWLQNTGGAIGIGLPAATGAAVACPDRKVICLQADGAGMYT
                     LQALWTQARESLDVVTVLFANRVYRILQGELAAVGAKPGPAADRLFGLAPPELDWLKL
                     ANGMGVEAARVETLEAFADVFRAACGRRGPFLIEFRI"
BASE COUNT          517 a          937 c          997 g          512 t
ORIGIN      
        1 tcggcagtgc cggtcgcgtc gcgcgagaac agaccctgcg tgctcgtgag actgtcggtc
       61 ctgaaccaga actggatcgt gccgttcgcc ggcccggtcg cgtagccgct ggtctcgccg
      121 atgacgatgg cgtcatcgac gccgtcgaaa acgacggcag tgtcgctgtc gcccgcgatc
      181 gcacccggct ggccgagcag gacgccgccg agcaactgga tgtcagtggc cggcgacgag
      241 ccgatgtcga ccaggggcgc cgaagcctcg ccgagtctga agtagttgat tggcgagtcc
      301 gccagcactt cgtcggagta ggaagccgcg tcgtcgttgg cgaagcccga ggcaagatgc
      361 caggtgacga tgagctctgg ccggtaggtg agcgtgccgg tttccgatga ccggaacgta
      421 atgccgtcag tgccgctgtt gaggacggcc catccgtagt tgctggttgc gccatccgac
      481 caggcctgca acgtcgccac aaggttcgcg ccggtaaaga tcgcgtccac gttggcagcg
      541 acaccgtccc cgacaccggt caaggaagca tcggctgtcg cgaggtagtc gacgcccgag
      601 ttcaatccac cggtcaacgt attccaggtt gcggtcgact cgttccagtc gacgagcatc
      661 cggtaaaggt tgatggtgtc ggtggtgttt gacgtaccgt tggtggtcag gactacctgg
      721 acatcatcga tgatggcgcc gaggggtatc tggtcggcgc cggcgccaaa gatgttgtcg
      781 aacctgagta tcgattgact ctcaaagccc aggttcgcgt ccaggtcgac gtcgaaggtg
      841 gtgtttgcgc ccgacgtggc gtcgggcgtt tcttcccaca gcgtggtgtc ctgcgtgcca
      901 gcgtatgaat tgacaccctg ctggaacgag gtcgtcgcca gcaccgcgaa ccactcgtcc
      961 tgcacttccg gcacgaggcc gatcgacgct tcgatcgagc cggtggcgta ttccaggttc
     1021 caatcgccgc cgtaggccca gttgcccgtc gtgtcggtcg acgccgtgac gtcggcgccg
     1081 gtggcggcgg agagcgtgtc gacgaacgcg cggcccgcgt caccggcggc gatgtcgcag
     1141 ccgtagagca gaaggtcggc gcccggcgcg aggctcgcgc tccacgcccc gagctcggcg
     1201 gcgcgcgcga ccaccgtggt gagcgtgacg ctgccggcac cgaggtagag cttgcccgcc
     1261 tcgccgtgcg agacgatgtg gatcgcgctg atgtcgctgc ggccctcgag cgtctcggtg
     1321 atctgctgca ggccgtcgcg cagcggatcg aggacgacga cctcgacgtt gggcttcgcc
     1381 aggtcgacga gctgctggta gtcggtgacc ttcggatcga tgaagaggag ttcgtggccg
     1441 ccgttgctcg cttcggggtc gagcagcgcg tcgctcagcg acgattcgat gacttccgcg
     1501 gtgccgggct gaacctgctg cgcctcagcg agcgattgcg tgtcggtctg ctgcgtgctg
     1561 tcctgaaaat ccggcagcgg atcgagcgtg tccagctgct cgacggttgc agcggccgcg
     1621 ccgtcgaaca gcatgcgcag ctcgagcgcg agcgcgctgc ggcgtggtcg ccactgcggg
     1681 gtgctgcgtg ctttcttcgc agacacgctg ccgactcgca tctcactcct cattcgagtc
     1741 gacaggatat ttaggcaagt caataaccac gctcgactac gaccgttcct tgacaaaaaa
     1801 ttgatcgtta gaggcgatag ttataacaag aatgaagtag ttgctgtaag ccaatctttt
     1861 cacagtattt tatttgaacc aggggttgtc gttagaaagc cgcagatata acgcaacaca
     1921 ttgaagttaa aacgttttct ttaaaacgaa ggaaagcgcg ccgaatatcg gcgacgaacg
     1981 gcgcgaattg gccggtctca gcgccaggct gcacgggcga cacatgcgcc ggccgtggct
     2041 ttgactcagg ctactgattt aaaaaggtaa aaacgcatca gcgccttatg atgcgacgca
     2101 tcataagagg ctcagaaggt cccgcgcgga gcggccgagg ctagatgcgg aattcgatga
     2161 ggaaggggcc gcgccgcccg caagccgccc ggaacacgtc ggcgaaggcc tccagcgtct
     2221 cgacgcgcgc ggcctcgacc cccatgccgt tcgccagctt gagccagtcg agctccggcg
     2281 gcgcgaggcc gaacaggcga tcggccgcgg gtcccggctt tgcgccgacc gcggcgagct
     2341 cgccctgcag gatgcgatag acgcggttgg cgaacagcac cgtcaccacg tcgagcgatt
     2401 cgcgcgcctg cgtccatagc gcctgcagcg tgtacatgcc cgcgccgtcc gcctgcaggc
     2461 agatcacctt gcgatcggga caggcgaccg cagcgccggt cgccgccggc aggccgatgc
     2521 cgatggcgcc gccggtgttc tgcagccagt cgttgggcgc cgcgccgaag gtcggcgcga
     2581 acagcgcgcg ccccgaggtc actgcatcct cgacgacgat ggcgttctcc gggatgacgc
     2641 cggcaaaggc atgcgcgaag gcgagcggct cgaatgcgcc gcgcacgttt gccttcggcg
     2701 cctcaatcgc aggcaacggc acctcgcgcg gcgcgccgag ctcgtcggcg agcgcttcga
     2761 gcgcggcgac ggcatcttcc tccggccgcg ccagcacgtc gagcgcgcag tccttcggcc
     2821 atagcgtgct cggcttgccc ggataggcga agaacccgac cggcggcttc gccgccacca
     2881 gcaccagttg ctggatgccg gccagcacct cgagcgccag gtcgacgaca tagggaatgc
     2941 gatcgacggg cacgcgcccg cgg
//
LOCUS       DATDAA010000306         2904 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_477285,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000306 DATDAA010000000
VERSION     DATDAA010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477285"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VIS73_09390"
     CDS             complement(<1..898)
                     /locus_tag="VIS73_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEY5763404.1"
                     /translation="MPEASAAVAVREFADSPWRLHQPFEPAGDQPQAIEALIAGLADG
                     LSFQTLLGVTGSGKTFTMANVIARTGRPALVLAPNKTLAAQLYSEFREFFPENAVEYF
                     VSYYDYYQPEAYVPSRDLFIEKDSSINDHIEQMRLSATKSLIERRDCLVVATVSCIYG
                     IGDREDYHAMILHLREKDRIAQRDIIRRLAEMQYSRNDIDFQRGNFRVRGDVIDVFPA
                     EHAEHALRISLFDDEVERLDLFDPLTGQIERRLSRFTVYPSSHYVTPRSTVLRAIEAI
                     KQELRERIAFFDGQAKLIEAQRI"
     gene            1032..2243
                     /locus_tag="VIS73_09395"
     CDS             1032..2243
                     /locus_tag="VIS73_09395"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HEY5763405.1"
                     /translation="MSTSLFAGVELAPRDPILGLTEAFNADTRSTKVNLGVGVYYDDN
                     GKIPLLAAVRAAEEARLKAAPPRGYQPIEGPAAYNNAVQNLLFGNGAELISAGRVVTA
                     QALGGTGALRIGADFIKRFVPGAVVYISDPSWENHRALFEAAGFTVETYPYYDPSTRG
                     ANFAAMKAKLATLTVGSTIVLHACCHNPTGADLTTAQWTEVVEVCRERGLVPFLDMAY
                     QGFADGIEPDAAAVRAFTASGLSFFVSSSFSKSFSLYGERVGALTIVTQNKEETARVL
                     SQLKRVIRTNYSNPPTHGGAIVAAALSSPELRKLWEDELAGMRDRIRAMRSGLVEQLA
                     AKGVSQDFSFVTRQRGMFSYTGLSAEQVERLKTEFGIYAVSTGRICLAALNTKNLPYV
                     AEAVAAVLKTG"
     gene            complement(2295..>2904)
                     /locus_tag="VIS73_09400"
     CDS             complement(2295..>2904)
                     /locus_tag="VIS73_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEY5763406.1"
                     /translation="ESFQASATVSGNSVTTDKIEGRLFGGVVKGSLRFSWGESMSLET
                     DIASEFMNTPVLLTALGMPAVVDGQVDGRVGFSVQAENWSKLVSSVPMEGDFLATKGS
                     LRGMDLVEAVRRAGQQPYRGGGTKFDTLKGRFAWDGKRLRLSQIDLASGIVSAYGAVQ
                     IDDEGRIDGDVNVMIRASASRTREPVSFSGTIKDPLLTAGRQ"
BASE COUNT          527 a          982 c          860 g          535 t
ORIGIN      
        1 cgatccgctg cgcttcgatg agcttggcct ggccgtcgaa gaacgctatc cgttcacgca
       61 gttcctgctt gatcgcctcg atggcacgta gcaccgtcga ccgtggcgtc acgtagtggc
      121 tcgacggata gaccgtgaac cgcgacagcc ggcgttcgat ttgcccggtc aagggatcga
      181 agagatcgag gcgttcgact tcatcgtcaa agagcgagat gcgcagcgca tgctcggcat
      241 gctcggcggg aaagacgtcg atgacgtcac cgcgaacgcg gaagtttccg cgctggaagt
      301 cgatgtcgtt gcgcgaatac tgcatctcgg cgagacggcg gataatgtcg cgctgggcga
      361 tgcggtcctt ctcccgcaaa tgcaggatca tcgcgtggta gtcctcgcga tcgccgatgc
      421 cgtaaatgca ggaaaccgtt gccacgacca ggcaatcccg ccgctcgatc agcgacttgg
      481 tagctgacag ccgcatctgc tcgatgtggt cgttgatcga actgtccttc tcgatgaaca
      541 ggtcgcgcga cggcacgtag gcctcgggct ggtagtagtc gtagtacgag acgaaatact
      601 cgaccgcgtt ctccggaaag aactcgcgaa actctgaata gagctgcgcc gccagtgtct
      661 tgttcggcgc cagcaccagc gccggccggc ccgttcgggc gatgacgttg gccatcgtga
      721 aggtcttgcc cgatccggtt acgccgagca gcgtctggaa ggacaatccg tcggcaaggc
      781 cggcaatcag cgcttcgatc gcctgcggct ggtcacccgc cggctcaaac ggctggtgca
      841 ggcgccaggg actgtcagcg aactcgcgaa ccgcgacggc ggcacttgct tcaggcatcg
      901 atgcattcga ttcgttttcg gagcccgcat tatgcggcag gcactctgcg ggcagccgtt
      961 cggcccgtgt taagatttgc gccgtttttg ccgcggcccg cgcggtgacc acactgcctt
     1021 ctggagcttc gatgagcacc tcattgttcg ccggtgtcga actggccccc cgcgacccga
     1081 ttctcggcct gacagaggcc ttcaacgccg atacccgcag caccaaggtc aatctgggcg
     1141 tcggtgtcta ttacgacgac aacggcaaga tccccttgct cgccgctgtc cgggccgccg
     1201 aagaagcgcg actgaaggcc gcgccgcccc gcggctacca gccgatcgag ggccctgccg
     1261 cctacaacaa cgccgtccag aacctgctgt tcggcaacgg cgccgaactg atttctgccg
     1321 gacgcgtggt gaccgcgcag gcactgggcg gtaccggcgc actgcgcatc ggagctgact
     1381 tcatcaagcg ctttgttccg ggcgccgtcg tctacatcag cgatccgtca tgggaaaacc
     1441 accgggcgct gttcgaggcc gccggcttca ccgtcgaaac ttacccctac tacgacccat
     1501 cgacgcgcgg cgccaacttc gcggcgatga aggcgaagct cgctacactc accgtcggct
     1561 cgaccatcgt cctccacgcc tgctgccaca acccgaccgg cgccgacctc accacggcgc
     1621 aatggaccga agtcgtcgaa gtctgtcgcg agcgcggtct cgtgcccttc ctcgacatgg
     1681 cttatcaggg tttcgccgat ggcatcgaac ccgacgccgc ggccgttcgg gctttcacag
     1741 catcggggct gtcgttcttc gtctcgagtt cgttttccaa gtcgttctcg ctctacggcg
     1801 agcgcgtcgg cgcactgact atcgtcaccc agaacaagga agaaaccgcg cgggttctgt
     1861 cgcagctgaa gcgcgtgatc cgaaccaatt actcgaaccc gccgacgcac ggcggcgcca
     1921 tcgtcgccgc cgcgctgtcg agccccgaac tgcgcaagct ctgggaagat gaactcgccg
     1981 gaatgcgtga ccgcattcgc gccatgcgta gcggcttggt cgagcagctg gcggccaaag
     2041 gcgtcagcca ggacttctcc ttcgtcacgc gccagcgcgg tatgttctcc tatacggggc
     2101 ttagcgcgga gcaggtcgag cgactgaaga ccgagttcgg catctacgcc gtatcgacgg
     2161 gccggatctg tctcgcggca ctcaatacga agaatctgcc ctacgttgcc gaagcagtcg
     2221 ccgcagtcct gaagaccggc taaagcacta tcagcgcggt cgctgccccg cgtccagcga
     2281 ccggggctcg acccttactg gcgccctgcc gtcaacagcg ggtccttgat cgtcccgctg
     2341 aagctgaccg gctcgcgcgt ccgtgatgcc gatgcccgga tcatcacgtt gacgtcgcca
     2401 tctatccggc cctcatcatc gatctgcacg gcgccatatg cgcttacgat tcccgacgcc
     2461 agatcgatct gcgacaagcg aaggcgcttg ccgtcccagg cgaaacgccc cttcaatgta
     2521 tcaaacttgg tgccgccacc acggtagggc tgctgccctg cgcggcgcac cgcctcgacc
     2581 agatccatcc cgcgcaggct acccttggtc gcgaggaaat cgccttccat cggaacgctc
     2641 gacaccagct tcgaccagtt ctcggcctgc accgaaaagc ccacgcgccc atcaacctgc
     2701 ccgtcgacga cagcaggcat tccaagtgca gtgagcagta ccggcgtgtt catgaactcg
     2761 ctggcgatgt ccgtctccag cgacatcgac tcgccccagc tgaagcgcag gctacccttg
     2821 accacgccgc cgaagaggcg tccctcgatc ttgtcggttg tcacgctgtt gcccgacacc
     2881 gtcgcgcttg cctggaagga ctca
//
LOCUS       DATDAA010000307        10063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_92562,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000307 DATDAA010000000
VERSION     DATDAA010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10063
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92562"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VIS73_09405"
     CDS             complement(<1..224)
                     /locus_tag="VIS73_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY5763407.1"
                     /translation="MRCPFCAAEETTVVDTRINEDGDIVRRRRRCLQCDKRFTTYERA
                     EVRLPQIVKKNASRVDFDREKVSSSMWLALR"
     gene            complement(282..1532)
                     /gene="glyA"
                     /locus_tag="VIS73_09410"
     CDS             complement(282..1532)
                     /gene="glyA"
                     /locus_tag="VIS73_09410"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY5763408.1"
                     /translation="MFSAQDSLAKADPELWSAIQNENRRQEEHIELIASENYVSRPVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEHVDVAEQLAIDRLKKLFGAEAANVQANSGSQAN
                     QAVLMAFVKPGETIMGMSLAEGGHLTHGMALNMSGKWFNVVAYGLNDKEEIDYPAMEK
                     LAHEHKPKLIIAGASAYALRIDFERFARVAKAVGAIFMVDMAHYAGLIAAGYYPNPVP
                     HADVVTSTTHKTLRGPRGGVILMKAEHEKAINSAIFPGLQGGPLMHVIAAKAVAFKEA
                     AQPAFKKYQEQVIANARVMSRVLAEERGLRIVSGRTESHVFLVDLRAKNITGKEAEAA
                     LGRAHITVNKNAIPNDPQKPFVTSGIRIGSPAMTTRGFTEIESEKVAHLVADVLDAPT
                     DEAVIERVRGEVSALCKRFPVYGA"
     gene            complement(1663..2322)
                     /locus_tag="VIS73_09415"
     CDS             complement(1663..2322)
                     /locus_tag="VIS73_09415"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5763409.1"
                     /translation="MKHSENQVSSVARFCLIRHGETPWNIERRLQGHRDIDLNAAGRH
                     QAQATALWLAREPIDALYASDLLRARHTAERIGISLALECRTLTDLRERRYGLFEGLT
                     YAEAALQFPADYARFEARDPDFVPPGAGESLVAFYLRVTATLQGLASRHPGQTVVVVT
                     HGGVLDVVNRFVRHLPLSAPRDFPIPNAGLNWLSVRGGAWEIESWGEVAHLADLSLDE
                     L"
     gene            2453..3919
                     /locus_tag="VIS73_09420"
     CDS             2453..3919
                     /locus_tag="VIS73_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY5763410.1"
                     /translation="MLASLKALLSRCQDGVLSFWRASRRRYASVEASAVRANDRLWHF
                     WHHDPRVRQTRLIITSEITARVVFIGGAIVVGLAAVLFAIMTDHASDLFHDLLTRWPL
                     LPLVLTPAGFVIIVWFTRRFFPGTGGSGIPQTVAAAHVPPHTDLRPWLSLRIAIGKVF
                     LTIGAVACGASVGREGPTVQVGASLMLMLRPLRILGRVASRRNLVIAGGAAGIAAAFN
                     TPLGGIMFAIEELSRYRIFRANSLTLLAVIAAGLMSLALLGNYSYFGEANVTLPWPRG
                     IGAILVAGAVGGLFGGLFARLMIASATGLPSRLSNLRSQRPYRFAAICGLTVAALGIL
                     WGGLAFGTGYVDTRAALESGEALPYGAGLIKIATTWISYLSGIPGGIFAPSLGVGAGI
                     GSDLAEFFPSLPRAAIILLVMSAYLAGFTQAPITSFIIVMEMSSNNEMLAPLMTAAVV
                     GHLIARMVCPTPLYHALAVLLTPESLGPNEQGRRTRQG"
     gene            complement(3959..4303)
                     /locus_tag="VIS73_09425"
     CDS             complement(3959..4303)
                     /locus_tag="VIS73_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763411.1"
                     /translation="MFPPLFAEDEVDRALPASEVTHGDAKAPPSDEQLTAEAVAMLKS
                     LFPRIHDRLGATWGTGQCEAYLDRLILDDRGDRDGFPPEVLTALLILQRVPTKEFGSF
                     KRRDPWDVGFRK"
     gene            complement(4635..5117)
                     /locus_tag="VIS73_09430"
     CDS             complement(4635..5117)
                     /locus_tag="VIS73_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763412.1"
                     /translation="MSLENWELASYIVTVIGLPLAVFVFFVEQRKERDNEEEAGYQML
                     SDAYSDFLKVVLDNPDLRLRSAMHVADLTPEQRERMQIIFEMLVALFERAYLVAYEPA
                     MQGEKARRWNSWADYMGEWCRREDFRRLLPTLLQGEDPDFAAYIGRLAAAEKARAQSG
                     "
     gene            complement(5119..5502)
                     /gene="gloA"
                     /locus_tag="VIS73_09435"
     CDS             complement(5119..5502)
                     /gene="gloA"
                     /locus_tag="VIS73_09435"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEY5763413.1"
                     /translation="MRLLHTMIRVGDLERSIAFYTEVMGMRLLRRKDYPEGRFTLAFV
                     GYRPEEETAVIELTHNWDTDRYELGNAFGHLAVAVDDAKAACDAIRARGGKVVREAGP
                     MKHGGSVIAFVEDPDGSKIELIERA"
     gene            complement(5504..6109)
                     /locus_tag="VIS73_09440"
     CDS             complement(5504..6109)
                     /locus_tag="VIS73_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5763414.1"
                     /translation="MIRLPFLFLAALALPLPAHAETLEGDQRLAQQLVTVRCALCHGL
                     DGQSLSPLYPKLASQNAEYIVKQIFNFKTGQRESTVMRDVANTLSAADIRALANYFSR
                     LPITPHPPSDPEVAAVGRYIYFKGNPLSGVAPCVTCHGVYATGGAQMPRLAGQHADYL
                     ERQLKGFIDSSRSNDMSMHAVVRGLTELEIRGVSQYLSGQE"
     gene            complement(6161..6937)
                     /locus_tag="VIS73_09445"
     CDS             complement(6161..6937)
                     /locus_tag="VIS73_09445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEY5763415.1"
                     /translation="MAPKPLSTPLQIELPLWSVAEPPPAPKAGDSLRRLLIGDQWVTY
                     RLRRGRRRRLTLTVDADGLRIGAPLSLPLQAIEGFAASHHDWIVRKLGEWQARSRRTL
                     VAVADGIAIPVLGRQLLLRVVPGARCREWSADQLRLPATGDLPAALETALREHARSHF
                     QARVVHYTSRLGVAAPPLSIGAARTRWGSCSRAGIRLNWRLIHFRALLVDYVIAHEVA
                     HLREMNHGPRFWKLVGTLFPDYAACRAALREEADALPIYR"
     gene            complement(6900..7655)
                     /locus_tag="VIS73_09450"
     CDS             complement(6900..7655)
                     /locus_tag="VIS73_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5763416.1"
                     /translation="MKHLRATLFIIAVIAFSVPLTTLIVICWHLPWSWRVTLSMAWRR
                     CFMASVRGILGIHMKIKGRENIPPGPCIILSKHQSAWETVAVQEVFHPRWLVFVLKRE
                     LIKLPFIGWALAATRMISIDRSAGSTALEQIVEQGRNRLEAGFSIVVFPEGTRVAPGT
                     SRRYKGGGAHLAIATGYPVVPVAHNAGELWPRNAFVKTPGTVTLSVGPAIDPAGKSVD
                     ALTREVESWIEGEMRVISPQRYGAKAAVDAAAD"
     gene            complement(7652..8188)
                     /gene="gmhB"
                     /locus_tag="VIS73_09455"
     CDS             complement(7652..8188)
                     /gene="gmhB"
                     /locus_tag="VIS73_09455"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEY5763417.1"
                     /translation="MKIIVLDRDGVINQDSVHFIKSPAEWVPIPGSLEAIARLNQAGY
                     RVVVATNQSGIERGLFDMDTLNAIHDKMLKTLATVGGRIDAIYYCPHAAESDCACRKP
                     KPGMFRRIAECFNTDLEGVLAVGDGLRDLQAAAAAGATPCLVLTGKGEQTREAGRLPK
                     GTRTFPDLAAVVDELLEP"
     gene            complement(8198..>10063)
                     /gene="glyS"
                     /locus_tag="VIS73_09460"
     CDS             complement(8198..>10063)
                     /gene="glyS"
                     /locus_tag="VIS73_09460"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY5763418.1"
                     /translation="DAQGKPTPALLKKLAALGADESVVAGLRREGVGKAEALFYESVA
                     PGATLADGLQKAIEAALAALPIPKVMSYQLADGWSTVNFVRPAHALVALHGSEVVPVA
                     VLGLQAGRETRGHRFEAAVERVVIADADAYAETLARDGAVIASFAERRAEIARQLADA
                     ATKLGGGVRPIADEALLDEVTALVERPNVLVGQFESEFLAVPQECLILTMKANQKYFP
                     LLDPSGRLTNRFLLVSNIRPADPGAVIGGNERVVRPRLADAKFFFDQDRKKSLESRVP
                     GLAKVVYHSRLGTQGERVQRVAAIARAIADRLGGGSLTQQAEQAAVLAKADLLTDMVG
                     EFPELQGIMGRYYAQHDGLPAEVADAIEDHYKPRFAGDSLPRGRIGTVVALADKLETL
                     VGLFGIGQIPTGDKDPFALRRHALGVIRMLAEGELDLPLADLLAVAAQAFARVDGFGD
                     ATTALADFVYERLAGSLREQGYTAQEVDAVVSQRPQRLGDIPKRLAAVRSFAALPEAA
                     SLAAANKRVGNILKKVDGAVAASVSAGLLKEAAEVALAGALAEVGPRANGAFAAGDYT
                     GSLQALAALRAPVDRFFDDVMVNVDDPALRANRLGLLATLHGAMNQVADLSKLSA"
BASE COUNT         1692 a         3315 c         3321 g         1735 t
ORIGIN      
        1 cgcaacgcca gccacattga cgaggagacc ttctcacggt cgaagtcgac acgcgaggcg
       61 ttcttcttga cgatctgtgg caggcgcacc tcggcacgct cgtaggtggt gaagcgcttg
      121 tcgcactgca ggcagcgacg acggcggcga acgatatcgc cgtcctcgtt gatccgcgtg
      181 tcgaccacgg tcgtttcctc ggcagcgcag aacggacatc tcatgaatgc ctctctccct
      241 tgatgccgcg cctaaagcca ggccgaggca cgacgcagaa ctcaggcacc gtataccggg
      301 aaacgcttgc agagcgccga cacctcgccg cgcacgcgtt cgatcactgc ttcgtcggtc
      361 ggcgcatcga gcacgtcggc gaccaggtgc gccacctttt ccgattcaat ctcggtgaaa
      421 ccacgcgtcg tcatcgccgg cgagccgatg cgaatgcccg aggtaacgaa gggtttctgc
      481 ggatcgttgg ggatcgcgtt cttgttgacg gtgatgtggg cgcgcccgag cgcggcctcg
      541 gcttccttgc cggtaatgtt cttggcgcgc aggtcgacga gaaagacgtg cgattcggta
      601 cgcccggaga cgatgcgcag gccgcgctcc tcggcgagca cccgcgacat cacgcgggca
      661 ttggcaatca cctgctcctg gtacttcttg aacgccggct gcgcagcctc cttgaaggcc
      721 accgccttgg ccgcgatcac atgcatcagc gggccgccct gcagtcccgg aaagatcgcc
      781 gagttgatcg ccttttcgtg ttcggccttc atcaagatca cgcctccgcg gggaccacgc
      841 agcgtcttgt gcgtagtcga ggtgacgacg tcggcgtgcg gcaccggatt cgggtagtag
      901 ccggcggcga tcagcccggc gtaatgcgcc atgtcgacca tgaagatcgc gccgacggcc
      961 ttggcaacac gggcgaaacg ctcgaaatcg atgcgcagcg cgtaggccga ggcgccggca
     1021 atgatcagct tcggcttgtg ctcgtgggcg agcttctcca tcgccgggta gtcgatctct
     1081 tccttgtcgt tgaggccgta ggcgactacg ttgaaccact tgccgctcat gttgagcgcc
     1141 atgccgtgcg tcagatgccc gccctcggcg aggctcatgc ccatgatcgt ttcgccgggc
     1201 ttgacgaagg ccatcagcac cgcctggttg gcctgcgaac ccgaattcgc ctggacgttg
     1261 gccgcttcgg caccgaacag cttcttcagt cgatcgatcg ccaactgctc agcgacgtcg
     1321 acgtgttcgc aaccgccgta gtagcgcttg cctgggtagc cctcggcgta cttgttggtg
     1381 agttgcgaac cctgcgcctc catcaccggg cggctgacgt aattctccga cgcaatcagc
     1441 tcgatgtgtt cttcctgtcg gcggttttca ttctggatgg cgctccacag ttcgggatcg
     1501 gccttggcca agctgtcttg ggcggaaaac atgaaattcc tttcagagat ggcggcgcgc
     1561 cggccaaacg gcatgggcgg cttgaaaacg cgcaatttta tcatcggctt aggccgatcc
     1621 gggacgccgc gacgaatgcc gcggccgctt tcctgccgcg actcagagtt cgtcgagcga
     1681 aagatcggcc aagtgggcga cttcgcccca gctttcgatc tcccaggcgc cgccgcgaac
     1741 cgacagccag ttcaggccgg cattggggat cgggaagtcg cgcggtgccg agagcggcag
     1801 gtggcggacg aagcggttaa ccacatcgag aaccccgccg tgggtgacga cgacgaccgt
     1861 ctgccccggg tggcgggagg ccaggccctg cagcgtcgcg gtcactcgca ggtagaaggc
     1921 gaccagactc tcgccggcgc cgggcgggac gaaatcgggg tcgcgcgctt caaagcgggc
     1981 gtagtcagcg gggaactgca gcgccgcctc ggcgtaggtc agcccctcga aaagcccgta
     2041 gcgacgctcg cgcagatcgg taagcgtccg gcattcgagc gcgagcgata tgccgatccg
     2101 ctcggccgta tgacgcgccc gcagcagatc gctcgcgtaa agcgcatcga ttggttcgcg
     2161 cgccagccac aacgcggtgg cctgcgcctg atggcggccg gcggcgttga ggtcgatgtc
     2221 gcgatggccc tgcaggcgcc gctcgatatt ccacggtgtc tcgccgtggc ggatcaggca
     2281 gaagcgcgca acgctcgaaa cctgattctc cgaatgcttc atgaagtctg ctcgctgcaa
     2341 aaacgggcgg cggccgacgt ccggctgcca ggcaatttgg tccgcgagtg tagccaaagc
     2401 acgccggtca ggggagaata aaaaggatga tgcccgcaag ccccgccacg ccgtgctcgc
     2461 gtcactgaag gcgcttctct ctcgctgcca agatggcgtg ctgagcttct ggcgcgcatc
     2521 gcggcgccgc tatgccagcg tcgaggcaag cgccgtgcgt gctaacgacc gtctctggca
     2581 tttctggcat cacgacccgc gcgttcgcca gacgcgcctc atcatcacct ccgaaatcac
     2641 cgctcgagtc gtctttatcg gcggcgcgat tgtcgtcggc ttggccgccg tcctgttcgc
     2701 gatcatgacc gatcacgcca gcgatctgtt ccacgatcta ctgacgcgct ggcccctgct
     2761 gccattggta ctgacgcccg ccggcttcgt catcatcgtc tggttcaccc gacgcttctt
     2821 ccccggcacc ggcggcagtg gcatcccgca gaccgtcgca gcggcgcatg tgccgccaca
     2881 taccgacctg cgtccctggc tgtcgctgcg catcgccatc ggcaaggtct tcctgaccat
     2941 cggggccgtc gcctgcggtg cctcggtcgg ccgagaaggc ccgaccgtgc aggtcggcgc
     3001 ctcgctgatg ctgatgctgc ggccgctgcg catcctcggt cgggtcgcat cgcgacgcaa
     3061 cctggtgatc gccggcggcg cggccgggat cgccgccgcc ttcaacacgc cgctcggcgg
     3121 aatcatgttc gccatcgaag agttgagccg ttaccgcatc tttcgcgcga acagcctgac
     3181 tttgctggcg gtgatcgccg ccggtctgat gtcgctcgcg ctgctcggca actacagcta
     3241 ctttggcgag gccaacgtca cgctgccctg gccgcgcggc atcggtgcca tcctcgtcgc
     3301 cggcgcggtc ggcggcctgt tcggcggcct gtttgcgcgg ctgatgatcg cctcggccac
     3361 gggtcttccc agccggctgt cgaatctccg ctcccagcgg ccctaccgct tcgccgcgat
     3421 ctgcgggctg accgtggcgg ccctcggaat cctctggggc ggactggcct tcggcaccgg
     3481 ctacgtcgac acccgtgccg ccctcgaaag cggcgaagcc ctgccttacg gggccgggct
     3541 gatcaagatc gcgacgactt ggatttccta tctttcggga ataccgggcg ggatcttcgc
     3601 gccgtcgctg ggcgtcggcg ccggaatcgg cagcgacctc gccgagtttt tcccctcgct
     3661 gccacgcgcg gcgatcatcc tgctggtgat gtcggcctac ctcgccggct tcacgcaggc
     3721 gccgatcacc tccttcatca tcgtcatgga gatgagcagc aacaacgaga tgctcgcgcc
     3781 gctgatgacc gcggcggtgg tcggccacct gatcgcccgc atggtctgcc cgacaccgct
     3841 ctatcacgcg ctcgctgtgc tgctgactcc ggagtcgctg gggcccaatg aacaggggcg
     3901 gcgcacccgc caaggctgag accggccgcc gccagggcag gcgcgggaac cgggaagact
     3961 acttacgaaa cccgacgtcc caaggatcgc gacgcttaaa gctgccgaat tccttggtag
     4021 gcacgcgctg caggatcagc agggcggtca gcacttcggg cggaaaaccg tcgcggtcac
     4081 cacggtcatc gagaatcaag cgatcaagat aggcttcgca ctgcccggta ccccaggtcg
     4141 caccgagccg gtcgtgaatc cgcgggaaca aggacttgag catcgcaacc gcctcggcgg
     4201 tgagttgctc atcgctgggc ggcgccttgg cgtcgccatg cgtaacttca ctcgccggca
     4261 gggcgcgatc gacttcgtct tcggcgaaga gcggcggaaa caagctgcca ctgctgtcat
     4321 tcgggctttt cggcaaacaa tccacctgct gttcaatcct gtcgttgtac ccggaactgt
     4381 ccggttccag gcgcgttggc gcgcggggtg caattctgac cgatggaatg acccagtggt
     4441 tgcaccaatc atcgtgcgct gcggaattct gtcacgcgcg agccgtgcgt ttcggcaggc
     4501 cgcgctcccc ggcaacgttc ccaccggaac cgattgatcc acggcgaccg taatcggcgg
     4561 gcgcgggcgc gcttttcttg ttgcactgca atgcgcgaga ggacgtcggc gacgaaggaa
     4621 actgagacgt cgactcagcc cgactgcgcc cgtgcctttt cggcggcggc gaggcggccg
     4681 atgtaggccg cgaagtccgg gtcttcgccc tgcagcaagg tcggcagcag gcgccggaaa
     4741 tcctcgcggc gacaccactc gcccatgtag tccgcccatg aattccagcg ccgcgccttc
     4801 tcgccctgca tcgctggttc gtaggcgacc aagtaggcgc gctcgaacag cgccacgagc
     4861 atctcgaaga tgatctgcat gcgctcacgc tgttcggggg tcagatcggc aacgtgcatt
     4921 gccgaacgca gccgcaggtc gggattgtcg agaacgacct tgaggaaatc actgtaggcg
     4981 tcggagagca tctggtagcc cgcctcttcc tcgttgtcgc gttccttgcg ctgttcgacg
     5041 aagaacacga agaccgccag cggcaggcca atgacggtga cgatgtagct ggccagctcc
     5101 cagttttcca ggctcacgtc aggcgcgttc gatcagttcg attttggagc cgtccggatc
     5161 ctcgacgaag gcgatcaccg agccgccgtg cttcatcggc ccggcctcac gaacgacctt
     5221 gccgccgcgc gcgcgaatcg cgtcgcaggc ggccttggcg tcatcgacgg cgacggcgag
     5281 atgtccgaag gcattgccga gttcgtaacg gtcggtatcc cagttgtggg tgagttcgat
     5341 caccgccgtc tcttcctcgg ggcggtagcc gacgaaggcg agcgtgaagc ggccttcggg
     5401 gtagtccttg cgccgcagca ggcgcatgcc catgacttcg gtatagaagg cgatggagcg
     5461 ctccaagtcg ccgacgcgaa tcatcgtgtg cagcagtctc atttcattcc tgcccggaca
     5521 gatattggga aacgccgcgg atctcgagct cggtcagccc gcgtacgaca gcgtgcatcg
     5581 acatgtcgtt gctgcgggac gaatcgatga aacccttgag ctgccgttcg agatagtcgg
     5641 cgtgctgacc cgcaagccgc ggcatctggg cgccaccggt ggcgtagaca ccatgacagg
     5701 tcacgcaggg agcgaccccc gacagcggat tgcccttgaa gtagatgtag cgtccgaccg
     5761 cggcgacctc gggatcggag gggggatgcg gtgtgatcgg caggcggctg aagtagttgg
     5821 cgagcgcgcg gatatcggcg gccgaaagcg tgttggcgac gtctcgcatg accgtggatt
     5881 cgcgctggcc ggtcttgaag ttgaagatct gcttgacgat gtactcagcg ttctggcttg
     5941 ccagcttggg atagagcggg ctcaggctct ggccgtcgag tccgtgacaa agcgcgcaac
     6001 gcaccgtcac gagttgttgc gccaaacgct ggtcaccctc aagcgtttcg gcgtgggccg
     6061 gtagcggcag ggccaatgcg gcaaggaaca ggaaaggcag gcgaatcatc agcaatatcc
     6121 cggcagcgtg gaatcgatca tggccgcatt catagcacaa ctaacgatag atcggcagcg
     6181 cgtcggcctc ctcgcgcagc gcggcacgac aggcagcgta gtcgggaaag agcgtgccga
     6241 ccagcttcca gaagcgcggg ccgtgattca tctcgcgcag gtgcgcgacc tcgtgggcga
     6301 taacgtagtc gacgagcagc gcgcgaaagt ggatcaagcg ccagttgagg cggattccgg
     6361 cgcgcgagca actgccccag cgggtccgcg ccgcgccgat cgagagcggt ggcgccgcga
     6421 caccaaggcg tgacgtgtag tggacaacgc gcgcctggaa atggctgcgc gcgtgctcgc
     6481 gcagagccgt ttccagcgct gccggcagat cgccggtggc cggcagccgc aactgatcgg
     6541 ccgaccattc gcggcagcgg gcgccgggaa cgacccgcaa caacagctgc cggccgagca
     6601 ccggaatcgc gatgccatcg gccaccgcga cgagggtccg ccgcgatctc gcctgccact
     6661 caccgagctt gcgcacgatc cagtcgtggt gagaagcggc gaagccttcg atcgcctgta
     6721 gtggcagact caacggcgcg ccgatccgca ggccgtcggc atcgacagtc agcgtcaagc
     6781 ggcggcggcg gccacggcgc aggcggtagg tgacccactg atcgcctatc agcaggcggc
     6841 gcagcgaatc accggccttg ggcgccggtg gcggttcggc caccgaccag agcggcagct
     6901 caatctgcag cggcgtcgac agcggctttg gcgccataac gttgcggcga gatcacgcgc
     6961 atttcgccct cgatccagct ttcgacctca cgcgtcagcg cgtcgacgct cttgccggcg
     7021 ggatcgatcg ccggcccgac gctcagcgtg acagtccccg gagtcttgac gaaggcgttg
     7081 cgcggccaca gttcgccggc gttgtgcgca accggcacga ccgggtaacc ggtggcgatc
     7141 gccagatgcg cgccgccacc cttgtagcgg cggctggtgc cgggcgcaac gcgcgttccc
     7201 tcgggaaaga cgacgatcga gaatccggct tcgaggcggt tgcgcccctg ctcgacgatc
     7261 tgctcgagcg ccgtggagcc tgcgctgcgg tcgatcgaga tcatgcgcgt agcggccagc
     7321 gcccagccga tgaagggcag cttgatcagt tcacgcttga ggacgaaaac cagccagcgc
     7381 ggatggaaga cctcctgcac tgccaccgtt tcccaggccg actggtgctt gctgagaatg
     7441 atgcacggtc cgggcggaat gttctcgcgc cccttgatct tcatgtggat gccgagaatg
     7501 ccgcgcaccg aggccatgaa gcagcgtcgc caggccatgg aaagcgtgac ccgccaagac
     7561 cacggcagat gccagcagat gacgatgagc gtggtcagcg gcaccgagaa ggcgatgacg
     7621 gcaatgatga acagcgtggc gcgaaggtgt ttcatggttc gagcagttcg tcgacgaccg
     7681 cggcgaggtc ggggaaggtt cgggtgccct tgggcaggcg gccggcctcg cgcgtctgct
     7741 cgcccttgcc ggtgagtacc agacaaggcg tggcaccggc cgcggcagcg gcctgcaggt
     7801 cgcgcaggcc atcgccgacg gcgagaacgc cttcaaggtc ggtattgaag cattcggcga
     7861 tgcgccggaa catgccgggc ttgggcttgc ggcaggcgca gtcgctctcg gccgcgtgcg
     7921 ggcagtaata gatggcgtcg atccgcccgc cgaccgtggc cagcgtcttc agcatcttgt
     7981 cgtgaatcgc gttgagcgta tccatgtcga acaggccgcg ctcgattccc gactggttgg
     8041 tggcgacgac cactcggtag ccggcttggt tgaggcgggc gatcgcctcg aggctgccgg
     8101 gaatcggcac ccactcggcc ggcgacttga tgaaatgcac cgagtcctgg ttgatcaccc
     8161 cgtcgcggtc gagaacgatg attttcacgg ccgtcttcta cgccgaaagc ttggacaggt
     8221 cggcgacctg gttcatcgcg ccgtgtaacg tcgcgagcag gccgagccgg ttggcgcgca
     8281 gcgccggatc gtcgacgttg accatcacgt cgtcgaagaa acgatcgacc ggcgcgcgca
     8341 acgcggccag cgcctgcagc gagccggtgt agtcgccggc ggcgaaggcg ccgttggcgc
     8401 gcgggccgac ctcggccagc gcgccggcca gcgcgacttc ggccgcctcc ttgagcaacc
     8461 cggcgctgac gctggcggca acggcgccgt cgaccttctt caggatgttg ccgacgcgct
     8521 tgttggccgc ggccagcgag gccgcctcgg ggagcgcggc gaagctgcgc actgcagcga
     8581 gtcgcttcgg aatgtctccc aggcgctgcg gccgctggct gacgaccgca tcgacctcct
     8641 gcgccgtata gccctgctcg cgcaagctgc cggcaaggcg ctcgtagacg aaatcggcca
     8701 acgcggtggt tgcatcgccg aaaccgtcga cccgggcaaa tgcctgcgcg gcaacggcga
     8761 gcagatcggc cagcggcaga tcgagttcgc cttcggccag catgcgaatc acgcccagcg
     8821 cgtgacggcg cagcgcgaag gggtccttgt cgccggtggg aatctggccg atcccgaaca
     8881 ggccgaccag cgtctccaat ttgtcggcga gcgcgacgac ggtaccgatc cgcccgcgtg
     8941 gcaggctgtc gccggcaaag cgcggcttgt agtggtcctc gattgcatcg gcgacctcgg
     9001 ccggcaggcc gtcgtgctga gcgtaatagc ggcccatgat gccttgcagt tcagggaact
     9061 cgccgaccat gtcggtcagt aggtcagcct tggccagcac cgcggcctgc tccgcctgtt
     9121 gcgtcagcga gcccccgccg aggcggtcgg cgattgcgcg ggcaatcgcc gcaacgcgct
     9181 gcacgcgctc gccctgtgtg ccaagcctgc tgtgataaac gaccttcgcc agcccgggaa
     9241 cgcgcgactc gagcgacttc ttgcgatcct gatcgaagaa gaacttggcg tcggcaaggc
     9301 gcggacggac cacgcgctcg ttgccgccga tcaccgcgcc cgggtcggcc ggccggatgt
     9361 tgctgaccag caggaagcgg ttagtgagcc ttccggacgg gtcgagcagc gggaagtact
     9421 tctggttcgc cttcatcgtc aggatcaggc attcctgcgg cacggcgagg aattccgatt
     9481 cgaactgacc gaccagcacg ttgggtcgct cgaccagcgc cgtcacctcg tcgagcagcg
     9541 cctcgtcggc gatcgggcga accccgccgc cgagtttcgt ggcagcgtcg gcgagctgcc
     9601 gcgcgatttc ggcacggcgt tcggcgaagg acgcgatcac cgccccgtcg cgtgcgagcg
     9661 tctcggcata ggcgtcggcg tcggcgatga cgacgcgctc gacggccgcc tcgaagcgat
     9721 ggccccgcgt ctcccggcca gcctgcagtc ccaaaactgc taccgggact acctcgctac
     9781 cgtgaagcgc caccagcgca tgcgcgggcc gcacgaagtt cacggtgctc catccgtcgg
     9841 caagctgata gctcatcacc ttcgggattg gcagcgccgc gagcgccgcc tcgatcgcct
     9901 tctgcaagcc gtcggcaagc gtcgcaccgg gcgcgacgct ttcatagaac agcgcttcgg
     9961 ccttgcctac gccttcgcgg cgcaagccgg cgaccaccga ttcatcggca ccgagcgccg
    10021 ccagcttctt gagcagcgcc ggcgtcggct tgccctgcgc atc
//
LOCUS       DATDAA010000308         2288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_407527,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000308 DATDAA010000000
VERSION     DATDAA010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407527"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VIS73_09465"
     CDS             complement(<1..1088)
                     /locus_tag="VIS73_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEY5763419.1"
                     /translation="MTQESNCPFAGAHGARTTLGVQSNRDWWPKLLNLKILHQHSAPS
                     NPLGGDFNYADEFNKLDYGALKKDLTALMTDSQDWWPADWGHYGGLFIRMAWHSAGTY
                     RVADGRGGAGHGNQRFAPVNSWPDNGNLDKARRLLWPIKQKYGNRISWADLMILAGNV
                     ALESMGFNTFGFAGGREDIWEPEEDVYWGRETEWLGDKRYSGDRQLENPLGAVQMGLI
                     YVNPEGPNGNPDPVASGRDVRETFARMAMNDEETVALVAGGHTFGKAHGAGDPKLVGP
                     EPEGAAIEEMGLGWINRLGSGTGADTTTSGIEGAWKPNPTKWDMGYFDMLFGYEWELV
                     KSPAGAHQWLAKNVRDEHMIPDAHDPAK"
     gene            complement(1279..1830)
                     /locus_tag="VIS73_09470"
     CDS             complement(1279..1830)
                     /locus_tag="VIS73_09470"
                     /inference="COORDINATES: protein motif:HMM:NF018201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763420.1"
                     /translation="MLRGLEAASAAGFSPVKINMVVQAGVNDDEVGAMVEYCRARGFV
                     LRLIERMPIGSAASEAWQSLQGIRQRLIEEHALIDTVIPGGGPARYLGSPDGSLTIGF
                     ITPMSQHFCDTCNRVRLAANGDLYTCLDAAGATPLGVQLRAGANDASLRARIAEAIWD
                     RPESHDFGKGRDRKIRIMSVTGG"
BASE COUNT          384 a          728 c          749 g          427 t
ORIGIN      
        1 ttcttcgccg gatcgtgggc gtcggggatc atgtgctcgt cgcgcacatt cttcgccagc
       61 cattgatggg cgccggccgg gctcttcacc aactcccact cgtagccgaa gagcatgtcg
      121 aagtagccca tgtcccattt cgtcgggttc ggcttccacg cgccttcgat gccgctggtc
      181 gtggtgtccg cgcccgtgcc cgagccgaga cggttgatcc agccgagccc catttcttcg
      241 attgctgcgc cttctggctc gggcccgaca agcttgggat cgcccgcgcc gtgcgccttg
      301 ccgaaggtgt gtcccccggc gaccagcgcc acggtttcct cgtcgttcat cgccatgcgg
      361 gcgaaggtct cgcgcacgtc acggcccgag gcaaccgggt cggggttgcc attggggcct
      421 tcgggattca cgtagatcag gcccatctgc acggcgccga gcgggttctc gagctggcgg
      481 tcgccactgt agcgcttgtc cccaagccac tcggtttcgc gaccccagta gacgtcttct
      541 tcgggttccc agatgtcctc tcggccaccg gcaaagccga aggtgttgaa gcccatcgat
      601 tcaagcgcga cgttgccggc aaggatcatc aggtcggccc aagagatgcg gttaccgtac
      661 ttctgcttga tcggccagag caggcgccgt gccttgtcga ggttgccgtt gtcgggccag
      721 ctgttgaccg gcgcgaagcg ctggttgccg tggccggccc caccccgccc atcggcgacc
      781 cggtaggtgc cggcgctgtg ccaggccatg cggatgaaca ggccgccgta gtgtccccag
      841 tcagcgggcc accagtcctg cgagtcggtc atcagcgccg tcaggtcctt cttcagcgcc
      901 ccgtagtcaa gcttgttaaa ctcgtcggca tagttgaaat ccccgcccag cggattcgag
      961 ggcgcgctgt gctggtgcag gatcttcagg ttgagcagct tcggccacca gtcgcgattg
     1021 gactgcactc cgagcgtggt acgggcacca tgggcaccgg caaacggaca gttgctttcc
     1081 tgagtcatga tatttcctcc ggtaaacgtg aaaaggctgg gtgatggacc cagcctactg
     1141 ccgacgcttc ctggatgcca ttggaaaata ctattccgac gattggctcg cgctaattag
     1201 ccgccatcaa tgcctttccc gcgttgtgcc ggcgtaggca atgcgcgaat cgccagcgcg
     1261 gaaaacaacg tttgcctgct aaccgccggt taccgacatg atgcggatct tccggtcgcg
     1321 acccttaccg aagtcgtggg attccggccg atcccatatc gcctcggcga tccgcgcgcg
     1381 cagcgatgcg tcgttggctc cggcacgcag ttgcacaccg agcggcgtcg caccggcggc
     1441 gtcgaggcag gtgtagagat cgccgttagc ggcaagccga acgcgattgc aggtatcgca
     1501 gaagtgctgc gacatcggcg tgatgaaacc gatggtgagc gaaccgtcgg ggctgccgag
     1561 gtagcgcgcc gggccaccgc cgggaatcac ggtgtcgatc agggcgtgtt cttcgatcag
     1621 gcgctggcgg atgccctgca ggctctgcca ggcctcgctg gccgcgctgc cgatgggcat
     1681 gcgttcgatc aagcggagca cgaagccgcg ggcgcggcag tactcgacca tggcgccgac
     1741 ctcgtcgtcg ttgacgcctg cctggacgac catgttgatt ttgaccggcg agaaaccggc
     1801 ggcgctggcc gcttcaagcc cgcgcaacac gtcggccagg caatctcggc agacgatggc
     1861 ggccgaacga atcgaggctg acgttgaggc ggcgcacgcc ggcggcgcgc agcacgtcgg
     1921 gccgctcgca gaagccggct tggccattgg gaatgcagca gccgcagcgc agatcgcagc
     1981 ggtcggtgac ctatacgcgc aggcagctga tgcgccgccc gaaacggtcg gcgggcgtgg
     2041 tcgattgacc gtgttgacga agcgattgcg gctggctcaa gtgttggcct cggtggcgtg
     2101 ccgatgccta ccgatcgtac tggcgcaccg cggggccctc aagcgcgcag cgagcggcat
     2161 ttgatccatc tgtaaaaact cgcgcatgcc gcgcaattgg tgtttacttg aagcgaaagc
     2221 cgctccggag aacgagcgga tgcgcagtgg aggacagcat gaacttcaag gaacgcagga
     2281 cttaccgc
//
LOCUS       DATDAA010000309        12982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_232793,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000309 DATDAA010000000
VERSION     DATDAA010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12982
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232793"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VIS73_09475"
     CDS             <1..452
                     /locus_tag="VIS73_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY5763421.1"
                     /translation="YVIIGTAAVKNPGFLHDACGAFPGHIIVGLDARDGKVATDGWSK
                     LTGHDVIDLGKKYEDYGVEAIIYTDIGRDGMLSGVNVEATERLADALRVPVIASGGLS
                     SLQDVERLCAAAESGVIGTIAGRAIYDGSLDLAEAQKLADRLSGAAR"
     gene            452..1213
                     /gene="hisF"
                     /locus_tag="VIS73_09480"
     CDS             452..1213
                     /gene="hisF"
                     /locus_tag="VIS73_09480"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019868453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY5763422.1"
                     /translation="MQLAKRIIPCLDVTAGRVVKGTNFVELRDAGDPVEIAARYNEQG
                     ADELTFLDITASSDQRDIILHVVESVASQVFIPLTVGGGVRTIADVRRLLNAGADKVS
                     INTAAVQNPDVVAEAAGKVGRQCIVVAIDAKSVGEGKWEVFTHGGRNRTGIDAIDWAR
                     RVEALGAGEILLTSMDRDGTKAGFDLPLTRAVADAVGIPVIASGGVGNLQHLADGVTE
                     GRADAVLAASIFHFGEYTVRQAKEFMRDRAIEVRL"
     gene            1238..1645
                     /gene="hisI"
                     /locus_tag="VIS73_09485"
     CDS             1238..1645
                     /gene="hisI"
                     /locus_tag="VIS73_09485"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY5763423.1"
                     /translation="MSGDLDHSLSWLDALRWDADGLIPAIAQDADSGRVLMFAWMNRE
                     ALAETADTRRAVYWSRSRRRLWRKGEESGHFQQVRAIGTDCDGDVILLGIEQQGGIAC
                     HTGRASCFFNQLREDRWVAVDPVLKNPEEIYRK"
     gene            1642..1965
                     /locus_tag="VIS73_09490"
     CDS             1642..1965
                     /locus_tag="VIS73_09490"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEY5763424.1"
                     /translation="MKNVEVLDRLSETLLARRKADPDTSYTAKLLANAPDLILKKIGE
                     ESAELIMAAKDGKRLNIVWEVTDVWFHTLVLLAHYGLTAEDVLNELRRREGISGIDEK
                     AARNK"
     gene            1962..2312
                     /locus_tag="VIS73_09495"
     CDS             1962..2312
                     /locus_tag="VIS73_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEY5763425.1"
                     /translation="MSDCLFCRIAKGEIPSRKVFEDDEILAFHDINPQRPVHFLMIPK
                     RHIESLAQATAADAAIIGRMLVKANALAVEQGSPDGFRVIINTGRVGNQEVSHLHIHI
                     VGGTDPVGPMLKKL"
     gene            2325..2561
                     /gene="tatA"
                     /locus_tag="VIS73_09500"
     CDS             2325..2561
                     /gene="tatA"
                     /locus_tag="VIS73_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEY5763426.1"
                     /translation="MGAMSIWHWLIVLVIILLVFGTKKLRNIGQDLGGAVKGFKDGVK
                     EGTAEKTADGQQQIAGEGKQGETIDVEVKEKTRS"
     gene            2618..3028
                     /gene="tatB"
                     /locus_tag="VIS73_09505"
     CDS             2618..3028
                     /gene="tatB"
                     /locus_tag="VIS73_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEY5763427.1"
                     /translation="MFDIGFSELMILAVVALVVIGPERLPKVARTVGHLMGRFQRYVS
                     DVKSDIDRELRLDEMKKLREEVERQARGIETQVSSQMSAVQKDIEKPVADIAQLTADG
                     ECLDAAKPADSPVPEAAAPAPEQASLPPVAPKSS"
     gene            3025..3783
                     /gene="tatC"
                     /locus_tag="VIS73_09510"
     CDS             3025..3783
                     /gene="tatC"
                     /locus_tag="VIS73_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY5763428.1"
                     /translation="MSDEPQAPEEGFLQHLIELRDRLIRSLLAVLVIFICVFPFAKEL
                     YALLAQPLIAALPAGGQMIATDVVGVFLVPMKVALLVAFLIALPYVLFQVWAFVAPGL
                     YSHEKKLVLPLVAASVLLFFLGMAFAYFLVFPTVFKFMSSVAPEGVAWMTDIEKYLSF
                     AMTTFIAFGVTFEVPVLVIVLVRVGMVSVEKLKEIRPYVIVGAFVIGAIFTPPDVISQ
                     VMLAVPMWLLYELGIVMARVVGTRAGADKEAPSS"
     gene            complement(3792..4637)
                     /locus_tag="VIS73_09515"
     CDS             complement(3792..4637)
                     /locus_tag="VIS73_09515"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEY5763429.1"
                     /translation="MCQLLGMNCNVPTDICFSFTGFQARGGGTDFHADGWGIAFFEGK
                     GVRLFLDPEPSVSSRIADLVKAYPIHSLNVIAHIRRSTQGVVSLENTHPFQRELWGRY
                     WVFAHNGNLERRDDGGDMDPRPVGNTDSEWAFCGLMRSLQERFGNTAPERDALFDAVA
                     DYSRDIGRLGEFNFMLSNGDWLLAHCSTHLAYIVRRAPFAKAHLCDEDVTVDFNQVTT
                     PNDRVAVIASIPLTDNEAWKTMPAGSLWLFEQGEPVRQVETVRGPDKPPPTRNRPGYV
                     PPTTC"
     gene            complement(4646..5815)
                     /locus_tag="VIS73_09520"
     CDS             complement(4646..5815)
                     /locus_tag="VIS73_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY5763430.1"
                     /translation="MHRFWLIFAQAVTVTLAVFFVVTTLKPEWLGGVRLHTVVPVLEA
                     SAPSAGSASAGYRDAARKALPSVVHVFTSKEVPAPRHPLLDDPIFKHFFGDRFELETQ
                     RTNSLGSGVIVSPAGYILTNYHVIEAASDIEVALADSRSFRARVVGADPESDLAVLQL
                     TPKTGADLSTTPLPAVTFAPADSAQIGDVVLAIGNPFGVGQTVTMGIVSALGRSHLGI
                     NTFENFIQTDAAINPGNSGGALIDTSGNLLGINTAIYSQSGGSLGIGFAIPVSSARAI
                     MEQIIQTGSVTRGWIGVEAQEITEELAEAFGLPNARGALIAGVLRGSPADDAGIRPGD
                     ILLAVGGKAIDDPQAMLESIAALKPGDSVPFSIRRDNVTREQPVRIGKRPPIRRQ"
     gene            5861..6604
                     /locus_tag="VIS73_09525"
     CDS             5861..6604
                     /locus_tag="VIS73_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEY5763431.1"
                     /translation="MQRDELLRHLDATLEAPRFKDYCPNGLQVEGASEVRRIVCGVSA
                     SQALIEAAVEIGADTLLVHHGWFWRGEDPRVLGMRRRRLATLLAHEFNLIAYHLPLDA
                     HSEFGNNAGLAKACGWRVEGRFLDQEIGYFGAAADDSVAALAARLETVLGRQPTVVGN
                     TGRALRRIAWCTGAAQDSLEAAVAIGADAFISGEISERTVLAARECDIAYLAAGHHAT
                     ERFGPIALAEHLGARFGLDARFIDIDNPA"
     gene            6635..7318
                     /locus_tag="VIS73_09530"
     CDS             6635..7318
                     /locus_tag="VIS73_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY5763432.1"
                     /translation="MKTVIPAWEHPSLSVAGTDARFPLRRIFCVGRNYADHAREMGHD
                     PTREPPFFFTKPADAAIQCETVAYPPATADLHHEIELVVALKAGGADLKPESALDCVF
                     GYAVGVDLTRRDLQAKAKAKGHPWDMAKGFDQSAPIGIVQPATEIGHPQRGAITLAVN
                     GETRQQGDLAQMIWSIPEVIAELSRLVRLAPGDLIFTGTPAGVSAIVCGDRVSGEVDG
                     VGRVEFTLV"
     gene            7459..10080
                     /gene="alaS"
                     /locus_tag="VIS73_09535"
     CDS             7459..10080
                     /gene="alaS"
                     /locus_tag="VIS73_09535"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY5763433.1"
                     /translation="MKSAEIRQAFLKFFESKGHQIAASSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYKRATTSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RDAIRYAWELLVEVFKLPKDKLWVTVYAEDDEAFDIWTKEIGVPVERCIRIGDNKGAR
                     YASDNFWMMGDTGPCGPCTEIFFDHGEHIPGGPPGSPEEDGDRYIEIWNNVFMQFNRD
                     EAGVMHPLPKPSVDTGMGLERIAAVLQHVHSNYEIDLFQKLIAAAGRETGQGNLDQPS
                     LRVLADHIRACSFLLADGVIPGNEGRGFVLRRIIRRAIRHGWKLGARAPFFHRIVPDL
                     VAEMGEAYPELKATQSRVMDVLKLEEERFFATIEHGMAILEGELAAMAATGATVFNGE
                     TAFKLHDTYGFPLDLTADICREKQVSVDSAAFDVAMARQKEQARAAGKFRMSAQLDYD
                     GPATTFLGYDTLEAKGNVLALYRDGSAVNELKEGELGVVVLDQTPFYAESGGQVGDRG
                     ALQSVHGIFAVEDTQKIQAAVFGHHGVVRTGTLTVGNGVTARVDLVARARTARHHSAT
                     HLMHKALREVLGAHVQQKGSLVDPEKTRFDFAHNQPMTAAETRRVESLVNAEILANTA
                     TEAKVMPLEEAQKSGAMMLFGEKYGDEVRVLSIGSSTELCGGTHVGRSGDIGLLKIVS
                     EGGVAAGVRRVEAVCGDVALAWIDSQQDALGAVTHALKVQPQEVAARVGQLADQVKAL
                     DRELARLKSKMAAAQGDDLAAQAIEVKGTRVLAALLEGADVATLRETIDKLKDKLKSA
                     AIVLGAVADGKVTLIAGVTSDLTGKLRAGDLVNMVAQQVGGKGGGRPDMAQAGGTDPA
                     ALPKALASVPDWVRSRC"
     gene            10088..12790
                     /locus_tag="VIS73_09540"
     CDS             10088..12790
                     /locus_tag="VIS73_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5763434.1"
                     /translation="MAADETHAKPALASPRIFSLTRIRWLSALMFLLMLSVGASVLWV
                     DWRSLVQVRQHAGSEVVNAQAAAIQLRLTTAVSATYALAAVLRQHDYQVEPAAFEALA
                     GELAEYHAGVSSLQYAPDGIIQHIVPLAGNEAAIGHNLMAHTERNREARLAIETRRLT
                     LAGPFSLVQGGVGLAGRLPIFRPTDKGKVEFWGFAIALIRVPELLQAAGIGHLRELGY
                     RYRLWRLHPDTGRPHVFAADQSIEGDLSDLVDPVTIDFEVPNGRWHLSAEPVEGWLSP
                     TRSDFLLKGVLVLFVALALTLTLYTLLRQPLILGDLVTRRTRELEETNTRLAEEIAVR
                     RQAEEQLRLGAMVFESSAESIMITDRSDRIVSVNDAFTRITGFTREEALGQPAILLDS
                     GRHDAAFLKVMDEALVRDGRWQGEIWSRRKSGELFPQWLGISVLSDENGNVTHYVSIA
                     SDISERKASAERIHYLAHYDSLTGLPNRVLLRDRVQQAMVHADRQHTKIGLMLLDLDR
                     FKVVNESLGHDLGDQLLLRVVDRLREAVRNTDTISRQGGDEFLIVVPDQRGAEVAARI
                     AEKIQRLLAQPFEIGEHEVRVTASIGIAIYPDDGVDFDTLMKLCDIAMYFAKESGRAT
                     YRLYTQAMKDDSVDRLAIEAELSHAVEEKQFELHYQPQIHLDGDRVIGAEALIRWRHP
                     EKGLIPPGRFIPVAEETGLIGPIGDWVLREACRQAVAWRAEGLPPLLVSVNLSGIQFR
                     SEGLVEQVATVLRETGLEAEYLELELTESTLLQDVESVMRTMRELKAVGVKLAIDDFG
                     TGYSSLSYLKRFAVDKLKIDQSFIRDLCIDSDDAAIVQAVIQLGQSLNLQTIAEGTET
                     REQVDALRAKGCMQVQGYYFSKPLPPEQFAAFLRNFPSGDATGG"
BASE COUNT         2253 a         4158 c         4290 g         2281 t
ORIGIN      
        1 cctacgtcat catcggcacc gccgcggtga agaaccccgg tttcctgcac gatgcctgcg
       61 gcgcctttcc cgggcacatc atcgtcgggc tcgatgccag ggacggcaag gtggcgaccg
      121 acggctggtc gaagctgacc ggccacgacg ttatcgacct cggcaagaag tacgaggact
      181 acggggtcga ggccatcatt tataccgaca tcggtcgcga cggcatgctt tcgggcgtga
      241 acgtcgaggc gaccgagcgc ctggctgacg cgttgcgcgt accggtcatc gcctcgggcg
      301 ggctgtcgtc gctgcaggat gtcgaacggc tgtgcgctgc cgccgaatca ggcgtcatcg
      361 gcaccatcgc cggtcgtgcg atctacgacg gttcgctcga cctggccgaa gcccagaagc
      421 tcgccgatcg cctgtccggc gcggcccgat agtgcagctt gccaagcgca tcatcccctg
      481 cctcgacgtc accgccgggc gggtggtgaa ggggaccaac ttcgtcgaac tgcgtgatgc
      541 cggcgatccg gtcgagatcg ccgcgcgcta caacgaacag ggcgccgacg aactgacctt
      601 cctcgacatc acggccagtt ccgaccagcg cgacatcatc ctccacgtcg tcgagtcggt
      661 tgcctcgcag gtcttcattc cgttgacggt cggtggcggc gtgcgcacga ttgccgacgt
      721 gcggcgcctg ctcaatgccg gtgccgacaa ggtgtcgatc aacaccgcgg ccgtgcagaa
      781 ccccgatgtc gttgcagagg ccgctggcaa ggtcggccgc cagtgcatcg tcgttgccat
      841 cgatgccaag tcggtgggcg agggcaagtg ggaagtgttt acgcacggcg gccgcaatcg
      901 caccggcatc gacgctatcg actgggcgcg tcgcgtcgag gcgctcggcg ccggcgagat
      961 cctgctcacc agcatggacc gcgacggcac caaggcgggc ttcgacctgc cgctgacccg
     1021 tgccgtggcc gatgcagtcg gaattccggt gatcgcctcg ggtggcgtcg gcaatctgca
     1081 gcacctcgcc gacggcgtca ccgaaggccg tgccgacgcg gtgctggcgg cgtcgatctt
     1141 ccatttcggc gaatacacgg tccgccaggc taaggaattc atgcgcgatc gcgcgattga
     1201 ggtgcggctg tgagcgcctc gacgcgaggg tagtcgggtg agcggcgacc tcgaccactc
     1261 gctttcctgg ctggacgcgc tgcgctggga cgccgacggc ctgatcccgg ccatcgcgca
     1321 ggacgcggac agcggccgtg tgctgatgtt cgcctggatg aatcgcgagg cgcttgccga
     1381 aacggccgac acgcggcggg cggtatactg gtcgcgttcg cgtcgccggc tctggcgcaa
     1441 gggcgaggaa tcgggtcatt tccagcaggt gcgggccatt ggcaccgatt gcgacggcga
     1501 cgtgatcctg ctcgggatcg aacagcaagg cggaatcgcc tgccacaccg gccgcgcgag
     1561 ttgctttttc aatcagttgc gggaagatcg ctgggttgcc gtcgatccgg tgttgaagaa
     1621 tcccgaggag atctatcgga aatgaaaaac gtcgaagttc tcgatcgcct gtcggagacg
     1681 ctgctcgcac ggcgcaaggc cgaccccgat acctcctaca cggccaagct gctggccaac
     1741 gcgccggacc tgatcctcaa gaagatcggc gaggaatcgg ctgaattgat catggcggcc
     1801 aaggacggca agcgcctgaa catcgtctgg gaagtcaccg acgtctggtt ccacacgctg
     1861 gtgctgctcg cgcactacgg gcttaccgcc gaggacgtat tgaacgagtt gcgccgtcgc
     1921 gaaggcattt ccggaatcga cgaaaaggcg gcgaggaaca aatgagcgac tgcctgttct
     1981 gccggattgc gaagggcgag attccgtcgc gcaaggtctt cgaggacgac gagatcctcg
     2041 cctttcacga catcaacccg cagcggcccg ttcattttct gatgattccc aagcgccaca
     2101 tcgagtcgct ggctcaggcg accgctgccg atgcggcgat catcggccgg atgctggtca
     2161 aggccaacgc gcttgccgtc gagcagggca gtccggacgg atttcgtgtc atcatcaata
     2221 cgggacgggt cggcaatcag gaagtgtcgc acctgcatat ccacatcgtc ggcggcacgg
     2281 acccggtcgg gccgatgctg aagaaactct gaaggggcaa gaaaatgggc gccatgagca
     2341 tttggcactg gctgatcgta ctggtgatca tcctgctggt attcggtacc aagaaactgc
     2401 gcaacatcgg ccaggacctg ggcggtgcgg tcaagggctt caaggacggg gtgaaggaag
     2461 gcacggccga gaagaccgcc gacgggcagc aacagatcgc cggcgagggc aagcagggcg
     2521 agaccatcga cgtcgaggtc aaggaaaaga cccgctcctg atcccggctg gccggcgtca
     2581 tcgacggtga cgctgtgatc cggaccggac ccaacgcatg tttgacatcg gtttctccga
     2641 gttgatgatt ctcgcggtcg tggcgctcgt cgtcatcggc cccgagcgcc tgcccaaggt
     2701 tgcgcgcacc gttggccacc tgatggggcg ctttcaacgt tacgtcagcg acgtcaagtc
     2761 ggatatcgac cgcgaacttc gcctcgatga gatgaagaag ctgcgcgagg aggtcgagag
     2821 gcaggcgcgc ggcatcgaaa cgcaggtttc cagccagatg tcagccgtac agaaggacat
     2881 cgagaagccg gtcgccgaca tcgcccagct gaccgccgac ggcgagtgcc ttgatgccgc
     2941 gaagcctgcc gattcgcccg tgccggaagc cgctgctccc gcgccggagc aggcctcgtt
     3001 accgccagtc gcaccgaaaa gctcatgagt gacgaaccgc aagcgccgga agaaggcttt
     3061 ctccagcacc tgatcgaact gcgtgaccgg ttgattcgtt cgctgctcgc ggtgctggtg
     3121 atcttcatct gcgtctttcc gtttgccaag gaactctacg cgctgcttgc gcagccgctg
     3181 atcgcggcgc tgccggccgg cggccagatg atcgccaccg acgtggtcgg cgtcttcctc
     3241 gtgccgatga aggtcgcgct tctggtcgcc ttcctcatcg cgttgcccta cgtgctcttc
     3301 caggtctggg cctttgtcgc gccaggcctg tactcgcacg agaagaagct ggtgctgccg
     3361 ttggtcgcgg cctcggtgct gctcttcttc ctcggcatgg ccttcgccta cttcctggtg
     3421 tttccgaccg tcttcaagtt catgtcatcg gtggcgcccg aaggcgtcgc ctggatgacc
     3481 gacatcgaaa aatacctgtc ctttgcgatg accaccttca tcgccttcgg agtcaccttc
     3541 gaggtgccgg tgctggtgat cgtgctggta cgcgttggca tggtcagcgt cgagaagctc
     3601 aaggaaatcc gcccctatgt gatcgtcggc gctttcgtca tcggcgcgat cttcacgccg
     3661 cccgacgtca tctcgcaggt catgctggca gtgccgatgt ggttgctcta cgaactcggc
     3721 atcgtcatgg cgcgcgtcgt cggcacacgc gccggcgcgg acaaggaagc gccctcttcc
     3781 tagccgctct gctagcaggt cgtcggcggg acgtagccgg gccggttgcg cgtcggcggc
     3841 ggcttgtcgg gaccccgcac cgtttccacc tgccgcaccg gttcgccctg ctcgaacagc
     3901 cagaggctgc cggccggcat cgtcttccac gcctcgttgt cggtcagcgg aatgctggcg
     3961 atcaccgcga cgcggtcgtt tggtgtcgtt acctggttga aatcgacggt gacgtcctcg
     4021 tcgcagaggt gtgccttggc gaacggcgcg cggcggacga tgtaggcgag gtgcgtcgag
     4081 cagtgcgcga gcagccagtc gccgttggag agcatgaagt tgaattcgcc caatcggccg
     4141 atatcgcggc tgtaatcggc aactgcatcg aacagcgcat cccgctcggg cgccgtattg
     4201 ccgaaacgct cttgcaggct gcgcatcagg ccacagaagg cccactcgct gtcggtattg
     4261 ccaaccggcc gcgggtccat gtcgccgccg tcgtcgcgtc tttcgaggtt gccgttgtgc
     4321 gcaaaaaccc agtagcggcc ccacagttcg cgctggaagg ggtgggtgtt ctccagcgaa
     4381 accacgcctt gggtcgagcg tcggatgtgc gcgatgacat taagcgagtg aatcgggtag
     4441 gccttgacca gatcggcgat ccgcgaggaa accgagggct ccgggtcgag gaagaggcgt
     4501 acgcccttgc cctcgaagaa cgcgatgccc cagccgtccg catgaaagtc ggtgccgccg
     4561 ccgcgtgcct ggaatccggt aaacgaaaag cagatgtcgg tcgggacatt gcagttcatg
     4621 ccgagcagct gacacatttg tggacctact ggcggcggat tggtggccgc ttgccgatcc
     4681 gtaccggttg ctcgcgcgtg acgttgtcgc ggcggatgct gaagggtacc gagtcgccgg
     4741 gcttcagcgc agcgatcgat tcgagcatgg cctgcggatc gtcgatcgct ttaccgccga
     4801 cggcgagcag gatgtcacca gggcggatgc ctgcgtcgtc ggccgggctg ccgcgcagga
     4861 cgccggcgat cagcgcgcca cgcgcgttcg gcaggccgaa ggcctcggca agctcctcgg
     4921 tgatctcctg cgcttcgacg ccgatccagc cacgcgtgac gctgccggtc tggatgatct
     4981 gttccatgat cgcgcgtgcc gaggacaccg ggatcgcaaa gccaatgccg agcgagccgc
     5041 cgctttgcga gtagattgcc gtgttgatgc ccagcaggtt gccgctggta tcgatcagcg
     5101 cgccgcccga attgcccgga ttgatcgccg catcggtctg gatgaaattc tcgaaggtgt
     5161 tgattccgag gtggctgcgc ccgagggcac tgacgatgcc catggtcacg gtctggccga
     5221 cgccgaacgg gttgccgatc gccagcacga catcgccgat ctgagccgag tcggccgggg
     5281 cgaaggtgac cgcaggcaat ggcgtcgtcg aaagatcggc accggtcttg ggggtcagct
     5341 gcagcacagc gaggtcgctc tccgggtctg cgccgacgac tcgggcgcga aagctgcggc
     5401 tatcggcgag cgctacctcg atgtcactgg ccgcttcgat gacatggtag ttggtgagga
     5461 tgtagccggc cgggctgacg atgacaccac tgccgaggct gttggtgcgc tgggtttcga
     5521 gttcaaagcg gtcgccgaaa aagtgcttga agatcgggtc gtcgagcagc gggtggcggg
     5581 gcgccggcac ttccttgctg gtgaatacgt gcaccaccga gggcagcgcc ttgcgcgccg
     5641 cgtcgcggta gccggcgctg gcggaacccg ccgagggagc gctcgcctca aggaccggca
     5701 ccaccgtgtg cagccgtacg ccgccgagcc attctggctt cagcgtggta acgacgaaga
     5761 agaccgcaag cgttacggtg accgcctgcg cgaaaatcag ccagaatctg tgcacgttcg
     5821 aatgtgacgc tcaatgggaa gccaaggggg aaaccgctcg atgcaacgtg acgaactgct
     5881 gcgccatctc gatgccacgc tggaagcgcc tcgcttcaag gattattgcc caaacggcct
     5941 gcaagtggaa ggcgcgtccg aagtcaggcg catcgtctgc ggcgtcagcg ccagccaggc
     6001 cctgattgag gccgccgtgg aaatcggcgc cgacacgctg ctggtccacc acggctggtt
     6061 ctggcgcggc gaggacccgc gcgtgctggg catgcgccgg cgacggctgg cgacgctgct
     6121 ggcgcacgag ttcaacctga tcgcctacca cctaccgctc gacgcgcact ccgaattcgg
     6181 caacaacgcc ggcttggcca aggcctgtgg ctggcgcgtc gaggggcggt ttctcgatca
     6241 ggagattggc tattttggcg cggccgccga cgattcggtc gcggcgctgg cggcacgatt
     6301 ggaaacggtg ctcgggcgcc agccgaccgt ggttggcaac acgggtcggg cgctgcggcg
     6361 aatcgcctgg tgcacgggcg cggcgcagga ctcgctggag gcagctgtgg cgatcggcgc
     6421 cgatgccttc atctcgggcg agatatccga gcgcacggtg ctggccgcgc gcgaatgcga
     6481 catcgcctat ctcgccgccg ggcaccatgc gaccgagcgc ttcggtccga ttgcgctggc
     6541 tgagcacctc ggtgcgcgct tcgggctgga cgcgcgcttc atcgacatcg acaatccggc
     6601 ctgagcttcg gcgtcacgcc tgaaaggcac caacatgaaa accgtcattc ccgcctggga
     6661 acatccatcg ctgtcggtcg ccgggaccga tgcccggttt ccactgcggc gaatcttctg
     6721 tgtcggccgc aattacgccg accacgcgcg cgagatgggt cacgacccga cgcgcgagcc
     6781 gccgttcttc ttcaccaagc cggccgacgc cgcgattcag tgcgagactg ttgcctaccc
     6841 accggcgacc gccgacctgc accacgagat cgagctggtc gttgcgctca aggccggcgg
     6901 tgccgacctc aagcccgagt cggccctcga ctgcgtattc ggttacgcgg tcggcgtgga
     6961 tctgacgcgg cgtgacctgc aggccaaggc caaggccaag gggcacccct gggatatggc
     7021 caagggcttc gaccagtcgg cgccgatcgg catcgtgcag ccggcgacgg agatcggtca
     7081 cccgcagcgc ggtgcgatca cgctggccgt caacggcgag acccgccagc agggcgatct
     7141 cgcgcagatg atctggtcca tccccgaggt catcgccgaa ttgtcgcggc tggtccggct
     7201 ggcgccgggc gacctgatct tcaccgggac gccggccggc gtgtcggcga tcgtttgcgg
     7261 cgatcgcgtc agcggcgagg tcgacggcgt tgggcgcgtc gagtttacgc tcgtctgatc
     7321 gccatcgtcc gtgccgcggg gctttgcgcc gcgccgggcg gcggccccga tgctagaatc
     7381 cgcgctcccg tgaccgctcg cgcctgtgcg gcgcacgacg gtttgtctcc ggcacgttta
     7441 ttgcgagttc gagtccccat gaaaagcgcc gaaatccgcc aggctttcct caaattcttc
     7501 gagtcgaagg ggcaccagat cgccgcctcc tcgtcgcttg ttccgcacga cgacccgacg
     7561 ctcttgttca ccaacgccgg gatgaaccag ttcaaggacg tcttcctcgg tttcgacaag
     7621 cgcccctaca agcgggcgac gacttcgcag aaatgcgtgc gtgccggggg caagcacaac
     7681 gacctcgaga acgtcggcta cacggcgcgg catcacacct tcttcgagat gctcggcaac
     7741 ttcagcttcg gcgattactt caagcgcgac gcgattcgct atgcctggga actgctggtc
     7801 gaggtcttca agctgcccaa ggacaagctc tgggtcaccg tctacgccga ggacgacgag
     7861 gccttcgaca tctggaccaa ggaaatcggg gttccggtcg agcgctgtat ccgcatcggc
     7921 gacaacaagg gtgcgcgtta cgcctccgac aacttttgga tgatgggcga cacgggcccc
     7981 tgcggcccct gcaccgaaat ctttttcgac cacggcgagc acattcccgg cggccccccg
     8041 ggatcgcccg aggaggacgg cgatcgctac atcgagatct ggaacaacgt gttcatgcag
     8101 ttcaaccgcg acgaggccgg cgtcatgcat ccgctgccca agccctcggt cgataccggc
     8161 atggggcttg agcgcatcgc cgccgtgttg cagcacgtcc actccaacta cgagatcgac
     8221 ctgttccaga agctgattgc cgctgccggg cgcgagaccg ggcagggcaa tctcgaccag
     8281 ccttccctgc gcgtcctcgc cgaccacatc cgcgcctgtt ccttcctgct tgccgacggc
     8341 gtgattccgg gcaacgaggg ccgcggcttc gtgctgcgcc ggatcatccg ccgggcgatc
     8401 cgtcacggtt ggaagctcgg cgcacgtgcg cccttcttcc accgcatcgt tcccgacctg
     8461 gtagccgaaa tgggcgaggc ctatcctgaa cttaaggcca cgcagtcacg cgtgatggat
     8521 gtgctcaagc tcgaggaaga gcgtttcttc gccaccatcg agcacggcat ggcgattctc
     8581 gaaggcgaac tggcggcgat ggccgccacc ggcgcaacgg tcttcaacgg cgagaccgcc
     8641 ttcaaactgc acgacaccta cggcttcccg ctcgatctga ccgccgacat ctgccgcgag
     8701 aagcaggtca gcgtcgattc ggcggccttc gacgtggcga tggcgcggca gaaggaacag
     8761 gcgcgcgccg ccggcaagtt ccgcatgagc gcccagcttg actacgacgg accggcaact
     8821 acctttcttg gctacgacac gctcgaggcc aagggtaatg tcttggcgct ttaccgcgac
     8881 ggcagtgccg tcaatgaact caaggagggc gaactcggtg tcgtggtgct cgaccagacg
     8941 ccgttctacg ccgaatccgg cggccaggtc ggcgaccgcg gtgcgctgca gtcggtgcac
     9001 ggcatcttcg ccgtcgagga cacgcagaag attcaggccg ccgtattcgg ccaccacggt
     9061 gtcgttcgca ccggcacgct gacggtcggc aatggcgtca cggctagggt cgacctcgtc
     9121 gcccgcgccc gcacggcgcg ccaccactcg gcgacgcacc tgatgcacaa ggccctgcgc
     9181 gaggtgctcg gcgcgcatgt gcagcagaag ggctcgctgg tcgatcccga gaagacacgc
     9241 ttcgattttg cgcacaacca gccgatgacc gcggccgaaa cccgtcgcgt cgaatcgctg
     9301 gtcaacgccg aaatcctcgc caacacggcg accgaagcga aggtgatgcc gctcgaggaa
     9361 gcacagaagt ccggcgcgat gatgctcttc ggcgagaagt acggcgacga ggtgcgcgtg
     9421 ctgtcgattg gctcgtcgac cgaactctgc ggcggcaccc acgttggccg cagcggcgat
     9481 atcggcttgc tgaagatcgt ttccgaaggc ggcgtggctg ccggtgtccg tcgcgtcgag
     9541 gcagtctgtg gcgacgttgc gctggcctgg atcgattccc agcaggacgc cctcggtgcg
     9601 gtgacgcatg cgctcaaggt tcagccgcag gaggttgcgg cgcgcgtcgg ccagctcgcc
     9661 gatcaggtca aggcgctcga ccgggagctg gcgcggctga agtcgaagat ggccgccgcg
     9721 cagggcgacg acctcgccgc acaggcaatt gaggtcaagg gcaccagggt gctcgccgcg
     9781 ctgctcgagg gtgccgacgt ggcgacgctg cgcgaaacaa tcgacaagct caaggacaag
     9841 ctgaagagcg ccgcgatcgt tcttggcgca gtcgccgacg gcaaggtgac gctgatcgcc
     9901 ggggtcacat ccgacctgac cggcaagctc agggccggcg acctggtcaa catggtcgcc
     9961 cagcaggtcg gcggcaaggg tggtggccgg cccgacatgg cgcaggccgg tggtaccgat
    10021 ccggcggcgt tgccgaaggc gttggcctcg gttccagact gggtgcgcag caggtgctga
    10081 accgctgatg gcagctgacg aaacccacgc caagcccgcc ctggcgtcgc cgcgaatttt
    10141 ttcgcttacg cggatccgtt ggctgtcggc gctgatgttc ttgttgatgc tgagtgttgg
    10201 cgccagcgtc ctttgggtcg actggcgctc gctggtgcag gtcaggcagc atgccgggtc
    10261 cgaggtggtc aacgcgcaag cggcggcgat ccagctgcgg ctgacgacgg cggtgtcggc
    10321 gacctatgcg ctggcggcgg tgctgcgcca gcacgactat caggtggaac cggcggcctt
    10381 cgaggcgctg gcaggcgagc tggccgagta tcacgccggt gtaagcagcc ttcagtacgc
    10441 gcccgacggc atcattcagc acatcgttcc gctcgccggc aatgaggctg cgatcggtca
    10501 caacctgatg gcccacaccg aacgcaaccg cgaggcgcgg ctggcgatcg agacacgtcg
    10561 cctgacgctc gccggcccct ttagcctggt ccagggcggc gtcggtctcg ctggccgact
    10621 gccgatcttt cggcccaccg acaagggcaa ggtcgagttc tggggttttg ccatcgcact
    10681 gatccgcgtg cccgaactgc tgcaggcggc cgggatcggg cacctcagag aactcggtta
    10741 tcgctaccgg ttgtggcgcc tgcatcccga taccggccga ccgcatgtct ttgccgccga
    10801 ccagtcgatc gagggcgact tgtccgatct cgtcgatccg gtgacgatcg acttcgaggt
    10861 gcccaacggc cgttggcatc tgagcgccga accggttgag ggctggttgt cgccaacccg
    10921 cagcgacttc ctgctcaagg gcgtgctggt gctcttcgtc gcgctggcgc tgacactgac
    10981 actgtacacg ctgctgcgcc agccgctgat tctgggggat ctggtgacac gccgaacgcg
    11041 agagctggag gagaccaaca cccggctcgc cgaggaaatc gccgtgcgcc ggcaagccga
    11101 ggagcagtta cggcttggcg cgatggtctt cgagtcgagc gccgaatcaa tcatgatcac
    11161 tgaccgcagc gatcgtatcg tctcggtcaa cgacgcgttc acgcgaatca ccggttttac
    11221 acgcgaggaa gcgctcggcc agccggcgat actcctcgat tccgggcggc acgacgccgc
    11281 tttcctcaag gtgatggacg aggcgctggt tcgtgacggt cgctggcagg gcgagatctg
    11341 gagccggcgc aagagcggcg agctgtttcc gcagtggctc ggcatcagtg tactcagcga
    11401 cgaaaacggc aacgtcacgc actacgtgtc gattgcctcc gacatctccg agcgcaaggc
    11461 atcggccgag cgcatccact acctcgcgca ctacgattcg ctgaccggcc tccccaaccg
    11521 tgtcttgctg cgcgaccggg tgcagcaggc gatggtacac gccgaccggc agcacaccaa
    11581 gatcggcctc atgctgctcg atctcgaccg cttcaaggtc gtcaacgaat cgctcggcca
    11641 cgatctcggc gaccagctct tgctgcgcgt cgtcgatcgc ctgcgcgaag ccgtgcgcaa
    11701 caccgatacg atcagccggc agggcggcga cgaattcctc atcgtcgtgc ccgaccagag
    11761 gggcgccgag gtggcggccc gaatcgccga gaagatccag cgcctgctcg cgcagccatt
    11821 cgagatcggc gaacacgagg tccgggtcac cgccagcatc ggcatcgcga tctatccgga
    11881 cgacggggtc gacttcgaca cgctgatgaa gctctgcgat atcgcgatgt acttcgccaa
    11941 ggaaagcggc cgcgccacct accgcctgta cactcaggcg atgaaggacg acagcgtcga
    12001 ccgcctggcg atcgaggccg agctgtcgca cgcggtcgag gaaaagcagt tcgagctgca
    12061 ctaccagccg cagattcacc tcgacggcga tcgtgttatc ggtgccgagg cgctgattcg
    12121 ctggcgccat cccgagaaag ggttgattcc gccgggacgt ttcatcccgg tggctgagga
    12181 aaccggcctg atcggcccga tcggtgactg ggtgctgcgc gaggcctgcc gtcaggcggt
    12241 cgcctggcgt gccgaaggcc tgccgccact gctggtttcg gtcaacctct cgggcatcca
    12301 gttccgcagc gaggggctgg tcgaacaggt ggcaaccgtc cttcgtgaaa ccggcctcga
    12361 ggccgagtat ctcgaactcg aactgaccga atcaacgctg ctccaggatg tcgagagcgt
    12421 catgcggacg atgcgcgaac tcaaggcagt cggcgtcaag ctggcgatcg acgacttcgg
    12481 caccggttat tcgagccttt cctacctcaa gcgtttcgcc gtcgacaagc tgaagataga
    12541 ccagtcgttc attcgtgacc tgtgcattga ctccgacgac gcggcaatcg tccaggcggt
    12601 catccaactc ggacagagcc ttaacctgca gacgattgcc gaaggcaccg aaacgcgcga
    12661 gcaggtcgac gcgctgcgcg ccaagggctg catgcaggtg cagggatact atttcagcaa
    12721 gccgctgccg ccggagcaat tcgctgcgtt tttgcgcaac ttcccgtcag gcgacgctac
    12781 aggcggttga ggcaggcggg gttcgccggt ggcgcttcag tcgtttcccg aattcttgcc
    12841 gatctggttg gcccaggcca gcgccgccgc cagggcgacc gtgaagactt cagtctgcag
    12901 catcgggaag gcgcgtagcc gcgccgtaac cacctcgggg tcttcggttt cggttgattc
    12961 gacgagcgcc agcagttcgc cg
//
LOCUS       DATDAA010000310         2087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_267070,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000310 DATDAA010000000
VERSION     DATDAA010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267070"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1992)
                     /locus_tag="VIS73_09545"
     CDS             complement(<1..1992)
                     /locus_tag="VIS73_09545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY5763435.1"
                     /translation="MRLFIILVLLISSLAVHADDSVLRNTDIFELEYAAEPQISPNGS
                     SVAYLRTSMDIMSDRAVPNIWQVDANGGNHRPLLSGPDNYSSPRWSPNGDRLAYVSGI
                     PGRGAQLHVRWMDTGQMGVISNVRQAPKALSWSPDGKHLAFVMFVRDDNEPLAKPPAA
                     PKGADWAPGAKVIETMPYRFDGRGYLETGHDHVFVVSAEGGTPRQLTSGDFDHRGPLA
                     WMPGGERIVFAANRLDDAEHHPVESELWAVTVASGELEQLTDRKGPDRGAAISPDGKR
                     IAYLGFDNKRLAWQSSDVYVMDVETRETRNLTAGFDRDIDDVQWAGSSNRLLVRFEDR
                     GRTHIGRLSLNGKVEPLVEDVGGTSIGRPYNSGSFSVANNGAFAYTAGRADRPADVGL
                     GRAGRKPARLTRLNDDLLGTKTLGSVEEITWKSSAGGLEIQGWLVYPPNFDPEQKYPL
                     ILEIHGGPHTAYGPHFSAEAQLYAAAGYVVLYANPRGSTSYGEKFSLEIHHDYPGEDY
                     DDLMSGIDAVIARGFVNEDALYVTGGSGGGVLTAWIVGKTDRFRAAVVAKPVINWTSF
                     TLTSDITHFVSRHWFPAMPWEDPSGYWERSPLSAVGNVSTPTALLTGEDDFRTPMPES
                     EQFYQALKLRKVDTALIRIPEASHGIAARPSQLIAKVDNI"
BASE COUNT          389 a          664 c          623 g          411 t
ORIGIN      
        1 aatattgtcg accttggcga tgagttgcga gggccgcgcg gcgatcccgt gcgacgcctc
       61 cggaattcga ataagcgccg tatcgacttt tctcagtttc agcgcctggt agaactgctc
      121 cgactccggc atcggcgtcc ggaaatcgtc ttcaccggtc agcagtgcgg tcggagtgct
      181 tacgttgcca acagcagaca gcggcgaacg ctcccaatac ccgctgggat cctcccacgg
      241 catcgccggg aaccagtgcc gggacacgaa gtgagtgatg tcggacgtca atgtaaagct
      301 ggtccagttg ataaccggct tggcaacgac ggctgcacgg aatcgatccg tcttgccgac
      361 gatccatgcc gtcagcacgc caccgcccga cccgcccgtt acgtacaggg cgtcctcgtt
      421 cacgaatccg cgtgcgataa ctgcatcgat accagacatc aggtcatcgt agtcctcacc
      481 gggatagtcg tggtgtatct ccaggctgaa tttctcgccg taactcgtcg aaccacgcgg
      541 gttggcgtac agcacgacat aaccggctgc cgcatacagc tgcgcctcgg ccgagaagtg
      601 cggtccgtag gcggtatgcg gcccgccgtg tatttcgaga atcaacgggt atttctgttc
      661 gggatcgaag ttgggcgggt acaccagcca gccctgtatc tcgaggccgc cggccgatga
      721 tttccaggtg atctcctcca cggaccccaa cgttttggtt ccgagtagat cgtcgttgag
      781 tcgcgtcaac cgggccggtt tgcggcctgc ccggcccagt cccacgtcgg cgggccggtc
      841 cgcccgtcct gccgtgtagg caaaagcgcc attattggcc acggaaaacg agccgctgtt
      901 gtaagggcgc ccgatggacg tccccccgac atcctcaacc agcggctcga ccttgccatt
      961 cagcgaaagg cgcccgatgt gggtgcgccc tcggtcctcg aagcggacca ggaggcggtt
     1021 ggacgaaccc gcccattgca cgtcgtcgat gtcgcgatcg aaaccagccg tcaggttgcg
     1081 cgtttcacgc gtttcgacat ccatgacgta gacgtccgag ctttgccacg ccagccgctt
     1141 gttgtcgaaa ccgagatagg caatccgctt gccgtcgggt gatatggcgg cgccgcggtc
     1201 cggtcccttg cggtcggtca gctgttcgag ctcaccgctg gcaaccgtca cggcccacaa
     1261 ttccgactcg accggatgat gctccgcatc gtccaggcga ttcgccgcga agacgatgcg
     1321 ctcgccgccc ggcatccacg cgagcgggcc gcgatggtca aaatcgccac tggtcagctg
     1381 cctgggtgta ccaccctcgg cggacacgac gaaaacatga tcgtgcccgg tttcgaggta
     1441 gcctcgacca tcgaatcggt agggcatcgt ttcgatgact ttggccccgg gggcccagtc
     1501 cgcacctttc ggtgccgccg gcggtttcgc aagtggttcg ttgtcgtcgc gcacgaacat
     1561 cacgaaagcg aggtgcttgc catccggtga ccagctcagt gctttgggcg cttgcctcac
     1621 gttgctgatt acccccatct ggcccgtatc catccagcga acgtgcagct gtgcgccacg
     1681 gccgggtata ccgctaacgt aagccaggcg gtcgccgttc ggcgaccagc gcgggctcga
     1741 gtagttgtcc ggccccgaca acagcggacg gtgattgccc ccgttcgcgt cgacttgcca
     1801 gatgttcggc acggcacggt cggacatgat gtccatcgag gtgcgcaggt aagcgacgct
     1861 ggacccattc ggggatatct gtggctcggc ggcatactcg agttcgaata tgtcggtgtt
     1921 tctcaggacc gagtcatcgg cgtggacagc cagcgacgaa atcaacagca ccaagataat
     1981 gaacagtctc atggaatccc cgctactcct gtaaagcggc gaagatagca tattgcccca
     2041 tttatcctgc agcgcagcaa tgcccctctt cagaacgtga ccgattg
//
LOCUS       DATDAA010000311         2102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_442572,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000311 DATDAA010000000
VERSION     DATDAA010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442572"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VIS73_09550"
     CDS             <1..469
                     /locus_tag="VIS73_09550"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEY5763436.1"
                     /translation="GGFTDVLLSRGAARVYAVDVGHGQLAWKLRNDDRVIVLERTNAR
                     HLTAEQVAQPVDIVVCDASFIGLEIVLPAPLALAAPGAWLVALIKPQFEVGRGEVGKG
                     GVVRDPAQHQAVCDRIRGWLDGLDGWHVVDIVESPITGPEGNREFLIAARKSG"
     gene            649..1500
                     /locus_tag="VIS73_09555"
                     /pseudo
     CDS             649..1500
                     /locus_tag="VIS73_09555"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(1866..>2102)
                     /locus_tag="VIS73_09560"
     CDS             complement(1866..>2102)
                     /locus_tag="VIS73_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044551230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY5763437.1"
                     /translation="GDEALRSALVCGAMAVVQQARRGRGNPTPWLAVLVARKEPKQAA
                     VALANKTARIAWKMMMTGEAWDPHRGRPPRPAAA"
BASE COUNT          350 a          759 c          659 g          334 t
ORIGIN      
        1 cggcggcttc accgacgtgc tgctgagccg cggcgcggcg cgggtctacg ccgtcgatgt
       61 cggccacggc cagctagcct ggaagctgcg caacgacgac cgggtgatcg tgctcgagcg
      121 taccaatgcg cgccatctga cggccgaaca ggtggcgcaa ccggtcgaca tcgtggtctg
      181 cgacgccagc ttcatcgggc tggagatcgt gctgccggcg ccgctggcgc tggccgcgcc
      241 cggcgcctgg ctggtcgcgc tgatcaagcc gcagttcgag gtcggcaggg gcgaggtcgg
      301 caagggcggc gtggttcgcg atccggcgca gcatcaggcc gtctgcgatc gcatccgcgg
      361 ctggctcgac gggctggacg gctggcacgt cgtcgatatc gtcgaaagcc cgatcaccgg
      421 gccggagggc aaccgcgagt tcctcatcgc ggcgcggaag tcggggtagg gcgcgttttc
      481 ctcttcgtcg ctcccgcgca ggcgggagcc tatgaacgcc gggcccttcc cggggttgtc
      541 ccatgttcat gggtccccgc attcgtgttt ggttgcgcgg gattggttgg acggaatcgg
      601 tgtagaccga ggcatctcgt tcattagaga gcgtctcgca tcatggacat gaaactgcac
      661 gccaacgcaa cgacgacccc gaagatccgt acctacatac agcagagcac ggcgccggtc
      721 gctgtgctgg ctgcagaact tggcgtcagc gagacgacgg tccgccgctg gcgcggccgc
      781 accacggtga ccgaccggtc gcatgcgcca acgacacgca ccaccacgct gtcgccggtc
      841 gaggagcggt tgatctgcga gctgcgccgc gaattggcac tgtcgctcga cgacatctgc
      901 gaggttatgc agcgctgcgt gcgcaccgcc atctcgcgca gcgccgtcca ccgctgcctc
      961 aagcgccatg gcctcaaccg ccagccgcgg cccgaaaggc cagcgcccgg cgccttcgaa
     1021 cagacgccgg tcggcttcat ccatatcgac ctgaagcatc tgcccgccct gcgccggcgc
     1081 aagagctatg ccttcgtcgc catcgaccgg gccacccgct tcgtctatgt cgaggtcgcc
     1141 gacagccgct cggccgagac catcgccgcc tgcctggtac gcttcctcga cgccttcccg
     1201 caaccggtcc gcaccatcct taccgacaat ggttcggagt tcaccgaccg ctttgccgtc
     1261 gacatgaagg acaagcccga cggcaaaccc tccggccgac accccttcga ccgcctgtgc
     1321 gccgaacgcg acatcgagca ccgcctgacc agaccctaca aaccgcagac caacggcatg
     1381 gccgagcgct tcaaccggcg gctcgccgaa gccctgcgca acgccacgcc cgatacccga
     1441 aacaccggca agaaccgctt cgccagccac gacgaacgca accgcttcat ccttgacttc
     1501 gttcatgcct acaatcgcac aagactgaaa tgcctcggct atcgcgctcc gattcaaacc
     1561 ctgcacaatc tcccggaaca caacacgcaa gcggggaccc atatgtggac ggcccccgta
     1621 cctgcaagag atttcgacga agacgatcgg atcgcttgcg atcatatgta cggcctttcg
     1681 gtgcggtcgc acatgaccgc cggccaagat ggatttcgcg acgcgaggtc caaacatgaa
     1741 cgcggcgtcg tggcgccgct gggtcccacg gagtgtctcg cgttccggat cgatcgatcg
     1801 ctccaccttc cctcctgcaa gttccggcaa cggcggggca gcagcgctgc gcccgccgat
     1861 ctcgttcagg cggcagccgg ccgcggcggc cggccgcggt gcgggtccca ggcctcgccg
     1921 gtcatcatca tcttccaggc gatgcgcgcg gtcttgttgg cgagcgccac ggcggcctgt
     1981 ttgggctcct tgcgcgcgac cagcacggcc agccacggcg tcgggttgcc gcggccgcgc
     2041 cgcgcctgct gcacgaccgc catcgcgccg cacaccagcg cgctgcgcag cgcttcgtcg
     2101 cc
//
LOCUS       DATDAA010000312         4325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_232991,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000312 DATDAA010000000
VERSION     DATDAA010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232991"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VIS73_09565"
     CDS             <1..500
                     /locus_tag="VIS73_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY5763438.1"
                     /translation="YVIEPTHTLPKFSYSHFGYSTQSSNFTKVSGKITLDRAAKKGSV
                     NVTIDAKSINTGVPLFNEHIQAADFFDTAMFDTITFVSDKMHFDGDKPASVDGKLTIK
                     GVTKPVTLKITSFHCMPHPIVKKDACGANAEAVVKRSDFNMSKYVPYVSDEVTLTIPV
                     EAIKE"
     gene            509..1369
                     /locus_tag="VIS73_09570"
     CDS             509..1369
                     /locus_tag="VIS73_09570"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEY5763439.1"
                     /translation="MTGAPLPAASLFVPHGAPTFALTPGAAGAALGRLGRELARPRAV
                     VVVSPHWDTATPAIGGALAPETIHDYGGFPRELYSLRYPATGNYCVASEVRDRLDDAG
                     IAAQIDLGRGLDHGAWVPLRFLYPDADVPVVPLSVQSRLGPTHHLALGRALAPLTADG
                     VLVVASGNLTHNLGDFMRSLQGAPPPAYVQQFPDWIANHLATGDIDELLDYRRRAPGA
                     TAAHPSDEHLLPLFVAIGAAAGESGAASASAEYRGIADAVLAMDLYRFTPASAAQARV
                     PHPLPERKTA"
     gene            1366..1611
                     /locus_tag="VIS73_09575"
     CDS             1366..1611
                     /locus_tag="VIS73_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEY5763440.1"
                     /translation="MSEKTEIPMKSPCAVNVEAGKSYWWCACGKSKSQPFCDGSHKGS
                     SFTPLEYKAEKSETVYFCGCKQTAGRPKCDGSHKKLA"
     gene            1645..2211
                     /locus_tag="VIS73_09580"
     CDS             1645..2211
                     /locus_tag="VIS73_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEY5763441.1"
                     /translation="MSKVAIVFHSGFGHTARQAQAVLEGAKREAGTAAKLYPVHEIGD
                     TQWAELAAADAIIFGAPTYMGSVSAPFKDFMDKSSKVWFTQGWKDKLAAGFTNSASQS
                     GDKLNSLIQLMVFANQHAMIWVGLGLMPGNNNSKGSVDDLNRLGSFTGAMSQSNNDQG
                     AEAMRESDLETARLLGQRVAQQVTRFAK"
     gene            2461..3924
                     /locus_tag="VIS73_09585"
     CDS             2461..3924
                     /locus_tag="VIS73_09585"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5763442.1"
                     /translation="MSTCFSTLGLAEPLLRAVADLGYTTATPVQAEAIPAALKGTDWM
                     VSSQTGSGKTAAFLLPCLNPLVEAGPQPMNALLGPSILVLCPTRELAQQVAAEAISLV
                     RHTRGIRVATVVGGMPFGKQIASLRGAAVVISTPGRLLDLVRRRQLRMDHVTRLVVDE
                     ADRMLDLGFVDDLDEIHKLCKNREQTLMYSATFAGRVMALAGRVMREPGRIELATAQD
                     RHTDITQTLHWADGLAHKKKLLDHWLRDAGLDQAVVFASTQADTEIIAGELAEIGHSV
                     AALHGAMPQALRNRRIKSLREGHIRVLVATDVAARGIDVPNISHVINFGLPMKAEDYV
                     HRIGRTGRAGRSGTAVTIAEHRERYKIRDIERFTQQTIAAQIIPGLEPSARPTPTRSG
                     PRNGSSFGKGPRPGGGYGGGYKGAGDGSRGQWQNRSADDRGGHQPRGEFRGEGRSDSR
                     GDSRGTWRNGPARQERSGGGSSWAGKSGRADRNDRGV"
     gene            3958..4197
                     /locus_tag="VIS73_09590"
     CDS             3958..4197
                     /locus_tag="VIS73_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763443.1"
                     /translation="MAIESVSSASVAAAPSTTLQPRQPTEDRNSVQAQPPDRQREPER
                     TQTNQAVQQAAEPDAQRPTVNSTGQTIGRIVNTTA"
BASE COUNT          817 a         1574 c         1309 g          625 t
ORIGIN      
        1 gctacgtaat cgaaccgacc cacacgttgc ccaagttctc gtacagccac ttcggctact
       61 cgacgcagag cagcaacttc accaaggtca gcggcaagat cacgctcgac cgcgccgcga
      121 agaagggctc ggtcaacgtg acgatcgatg ccaagtcgat caacaccggc gtgccgctct
      181 tcaacgagca catccaggcc gccgacttct tcgacacggc gatgttcgac accatcacct
      241 tcgtctccga caagatgcat ttcgacggcg acaagccggc cagcgtcgac ggcaagctga
      301 cgattaaggg cgtcaccaag ccggtgacgc tgaagatcac ctcgttccac tgcatgccgc
      361 acccgatcgt caaaaaggat gcctgtggcg ccaatgccga agccgtggtc aagcgctccg
      421 acttcaacat gagcaagtac gtcccctacg tcagcgacga agtcacgctg acgataccgg
      481 tcgaagcgat caaggagtaa gcaccgccat gaccggcgcg ccactgcctg ccgccagcct
      541 gttcgtgccg cacggggcgc cgaccttcgc gctgaccccc ggggcggccg gggcagcgct
      601 gggtcgcctc ggccgcgagc tggcaaggcc gcgcgccgtc gtcgtcgtct cgccgcactg
      661 ggacacggcg acgccggcga tcggcggcgc gctggcgccc gaaaccatcc acgactatgg
      721 cggctttccg cgcgagctct attcgctgcg ctacccggcc accggcaact actgcgtcgc
      781 cagcgaagta cgcgaccggc tagacgatgc cggcatcgcg gcgcagatcg acctcggccg
      841 cggactcgac cacggcgcct gggtgccgct gcgcttcctc taccccgacg ccgacgtgcc
      901 ggtagtgccg ctgtcggtgc agagccggct cggacctacc caccacctcg cgctcggccg
      961 tgcgctggcg ccgctgacgg ccgatggcgt gctggtcgtc gcctcgggca acctgacaca
     1021 caacctcggc gacttcatgc gcagcctgca gggcgcgcca ccgcccgcct acgtgcagca
     1081 gtttcccgac tggatcgcca accacctcgc caccggcgac atcgatgaac tgctcgacta
     1141 ccgtcggcgt gcccccggcg cgacggcggc gcacccgagc gacgagcact tgctgccgct
     1201 cttcgtagcc atcggcgccg ctgccggcga gtccggcgcg gcaagcgcga gcgccgaata
     1261 ccgtggcatc gccgacgccg tgctggcgat ggacctttat cgctttaccc ccgcgagcgc
     1321 agcgcaggcg cgcgtgccgc acccccttcc cgaaaggaaa accgcatgag cgaaaaaacc
     1381 gaaatcccga tgaaatcccc ctgtgccgtc aacgtcgaag ccggcaagtc ctactggtgg
     1441 tgcgcctgcg gcaagagcaa gtcgcagccg ttctgcgacg gctcgcacaa gggctcatcg
     1501 ttcaccccgc tcgaatacaa ggccgagaag agcgaaacgg tctacttctg cggctgcaag
     1561 cagacggccg gtcggccgaa atgcgacggc agccacaaga agctcgcttg aatcgaaaac
     1621 ctacaccgaa tctacggaga ccccatgagc aaagtagcaa tcgtttttca cagcggcttc
     1681 ggccacaccg cgcgtcaagc gcaggccgtc ctcgaaggcg ccaagcgcga ggccggcacc
     1741 gcggccaagc tataccccgt ccacgaaatc ggcgacacgc agtgggccga actggctgcc
     1801 gccgacgcca tcattttcgg cgcgccaacc tacatgggca gcgtctcggc gccgttcaag
     1861 gatttcatgg acaagagctc aaaggtctgg tttacgcagg gctggaagga caagctcgcc
     1921 gccggcttca ccaactctgc ctcgcaatcg ggagacaagc tcaactcgct gatccagcta
     1981 atggtcttcg ccaaccagca cgcgatgatc tgggtcggcc tcggcttgat gcccggcaac
     2041 aacaacagca agggcagcgt cgatgacctc aaccgcttgg gcagcttcac cggcgccatg
     2101 tcccagtcga acaacgacca gggggccgag gcgatgcgcg aatccgacct tgaaaccgcc
     2161 cgcctgcttg gccagcgggt cgcccagcag gtgacgcgct tcgcgaaata atcctcgctt
     2221 tccggccgcg actcctgcat aatgccgcgc cgcagcaaat attgactgcg gcgcggcgtt
     2281 ccagcggctc gagagagctc ccggatacga cgcctgaaac atcgccccca gccagggtgg
     2341 acgccacaag cgggccgccc gagtcgcaga atctgcattc cgtctgttgg tcgatgtctt
     2401 gccggatttc ggctcaccac tgacggagcc gcatcgccga tagcgatgcg ggaaaacccc
     2461 atgtctactt gtttctcgac gctcggcctg gccgagcccc tgctgcgcgc tgtcgcagat
     2521 ctcggctata cgaccgccac cccggttcag gccgaagcca ttcccgccgc cctcaagggc
     2581 accgactgga tggtcagtag ccagaccggc agcggcaaga ccgccgcctt cctgctgccc
     2641 tgcctcaacc cgctggtcga agccggcccg cagccgatga acgcgctgct cggcccgagc
     2701 atcctcgtcc tctgcccgac gcgcgaactc gcgcagcagg tggccgccga ggcgattagt
     2761 ctggttcgcc acacgcgcgg cattcgcgtc gccaccgttg tcggcggcat gcccttcggc
     2821 aagcagatcg ccagcctgcg aggcgccgcc gtcgtcatta gcacgccggg tcggctgctc
     2881 gacctcgttc gccgccgtca gctgcgcatg gatcacgtga cgcgcctcgt cgtcgacgag
     2941 gcggaccgca tgctcgacct gggcttcgtc gatgacctcg acgaaatcca caagctgtgc
     3001 aagaaccgcg aacagacgct gatgtactcg gccaccttcg ccggtcgcgt catggcgctg
     3061 gccggtcgcg tcatgcgcga accgggccgc atcgaactcg ccaccgcgca ggaccgccat
     3121 acagacatca cgcagacgct gcactgggcc gacggccttg cacacaagaa gaagctgctc
     3181 gatcactggc tgcgcgacgc cgggctcgac caggctgtcg ttttcgccag cacacaggct
     3241 gacaccgaga tcatcgccgg cgaactcgcc gagatcggcc actcggtcgc cgcactgcat
     3301 ggcgccatgc cgcaggccct gcgcaaccgc cgcatcaaga gcctgcgcga agggcacatc
     3361 cgcgtgctgg tcgccaccga cgtcgccgcc cgcggcatcg acgtgccgaa catcagccac
     3421 gtcatcaact tcggcctgcc aatgaaggcc gaggactacg tgcaccgcat cggccgcacc
     3481 ggccgtgccg ggcgcagcgg caccgccgtc accatcgccg agcaccgcga gcgctacaaa
     3541 attcgcgaca tcgagcgctt tacgcagcag accatcgccg cgcagatcat tcccggcctc
     3601 gagccgagtg cccgccccac ccccacccgc agcggcccgc gcaacggcag cagcttcggc
     3661 aagggaccgc gtcccggcgg cggctacggc ggcggctaca agggtgccgg cgacggcagc
     3721 cgtggccaat ggcagaaccg cagcgccgac gatcgcggcg gccaccagcc gcgcggcgag
     3781 ttccgtggcg aaggccgcag cgactcacgc ggtgattcac gcggcacttg gcgcaacggc
     3841 ccggcccggc aggaacgttc gggcggcggc agcagctggg caggaaaatc ggggcgtgct
     3901 gacagaaacg accgcggcgt ctaaactggg cctgtcagtt cacccggagt aatgctcgtg
     3961 gcaatcgaat ccgtctcatc ggccagcgtc gccgcagcac ccagcaccac gctgcaaccg
     4021 cgccagccga ccgaagaccg caattcggtt caggcccagc cgccggatcg gcagcgcgaa
     4081 cccgagcgca cgcagacaaa tcaggccgtg cagcaggcgg cggagcccga cgcgcaacgc
     4141 ccgaccgtca acagcaccgg gcagaccatt ggccgcatcg tcaacacgac ggcttgaact
     4201 ttagcagcgc ccacccgaaa gcggccttta ggccgctttt tctttggccg ctcagcatcg
     4261 gtgcgaaacg gacgacgggt gggtggattg cctgctttcg accctcagcg gaccttcgcg
     4321 ctccg
//
LOCUS       DATDAA010000313         2634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_92988,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000313 DATDAA010000000
VERSION     DATDAA010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92988"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="VIS73_09595"
     CDS             complement(<1..1063)
                     /locus_tag="VIS73_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5763444.1"
                     /translation="MSANPEDTRTTNAAWQERKLAAIARGQGNIAPVYIERGVNAELF
                     DVEGNRYIDFGTGIAVCNTGHSNPRVVAAVKDQVERFSHTCVMVTPYDSAVRLAEALN
                     ELAPGNTPKKSIFVTTGAEAVENTIKIARAYTGRRGVIAFNGGFHGRTLMAMGLTGKI
                     TPYKNLFGPFPGEVYHAPYPIAYHDVSVEDSLKAIDNLFKADIAPTDVAAIIVEPVQG
                     EGGFYPAPDEFLQALRAICDKHGIVMIADEIQTGFGRTGRFFASEYAGIEPDIITVAK
                     GVAGGYPLAAVTGKAEIMDAPLPGGLGGTYAGSPVGCAAALAVLEEIREHGLVDRALE
                     IGARFRARLEALQGKHPDIV"
     gene            complement(1073..2569)
                     /locus_tag="VIS73_09600"
     CDS             complement(1073..2569)
                     /locus_tag="VIS73_09600"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY5763445.1"
                     /translation="MSQALQEVASVDWSLSDPSLLRTQAYINGEWVDADDGATFQVLN
                     PATHEVVAEMANVGAAETRRAIEAAEVAQKAWAAKSAIERSGVLRKWYDLVMANVDDL
                     GILMTVEQGKILAEARGEVVYAASFIEWFAEEGKRVYGDVLPQPFTDRRGIVIKQPVG
                     VCAAITPWNFPAAMLTRKVAPALAVGCSMVAKPPQETPLSMTALAELADRAGLPAGLL
                     NVLPTNNSRSVGDEMCANPIVRKLTFTGSTGVGKILTKQCADTLKRLSMELGGNAPVI
                     VFDDADLKLAAAGAAASKYRNSGQTCVCANRILVQDTVYDEFIEELKKVVGTYKVGYG
                     LDESVTHGPIITRKALEDIKALVDDAIEHGATVAAGGEFGEGDSHFVEPTILCNVTPD
                     MRIFSEEIFGPIAPIFKFSTEEEAIEMANDTEFGLAAYVFTNSQGRSWRVAEGIEYGM
                     VGVNEALFSSTYIPFGGVKESGQGREGSYYGVDEYVETKFICLGNLNA"
BASE COUNT          430 a          864 c          830 g          510 t
ORIGIN      
        1 cgacgatatc cggatgcttc ccctgcaatg cctcgagccg cgccctgaag cgcgcgccta
       61 tctcgagcgc gcggtcgacg aggccatgct cgcggatttc ctcgagtacg gcgagcgccg
      121 cggcgcagcc gaccggcgaa ccggcgtacg tgccaccgag accgcccggc agcggcgcgt
      181 ccatgatctc cgccttgccc gtcacggccg ccagcggata gccgcccgcc acacccttgg
      241 ccacggtgat gatgtcgggc tcgatgcccg cgtactccga ggcgaagaac cggcccgtgc
      301 ggccgaagcc ggtctggatc tcgtcggcga tcatgacgat gccgtgcttg tcgcagatag
      361 cgcgtaatgc ctgcaggaac tcgtcaggcg cagggtagaa cccgccctcg ccctggaccg
      421 gctccacgat gatcgcggcg acatcggtcg gtgcgatgtc ggccttgaac aggttgtcga
      481 tggccttgag cgaatcctcg accgacacgt cgtggtacgc gatcgggtac ggcgcgtgat
      541 agacctcgcc cgggaacggg ccgaacaggt tcttgtacgg cgtgatcttg ccggtgagac
      601 ccatcgccat gagcgtgcgc ccgtggaagc cgccattgaa tgcgataacc ccgcgacgcc
      661 cggtgtaggc gcgcgctatc ttgatcgtgt tctcgaccgc ttctgcaccg gtggtcacga
      721 agatcgactt cttcggcgta ttcccgggag ccagctcgtt cagggcctcg gccaggcgca
      781 cggccgagtc atagggcgtc accatgacgc aggtgtggct gaaacgttcc acctggtctt
      841 tcacggccgc aacgacgcgc gggttgctgt gccccgtgtt gcagaccgcg atcccggtgc
      901 cgaagtcgat gtagcggttg ccttcgacgt cgaacagctc ggcattaacg ccacgctcga
      961 tatagacggg cgcgatgttg ccctgcccgc gcgcgatggc ggcgagtttg cgttcctgcc
     1021 atgcggcgtt ggtggtgcgg gtatcttcag gattggcgct catggtcttt actcacgcgt
     1081 tcaggttgcc gaggcagatg aacttcgtct ccacgtattc atcgacgccg tagtaggaac
     1141 cttcgcggcc ctggcccgac tccttgacgc caccgaaggg aatataggtt gacgagaaca
     1201 gcgcctcgtt gacgccgacc atcccgtact cgataccctc ggcgacgcgc catgagcggc
     1261 cctggctgtt ggtgaacacg tacgcagcca ggccgaactc ggtgtcgttg gccatctcga
     1321 tggcttcttc ctcggtgctg aatttgaaga tcggggcgat gggtccgaat atctcttcgc
     1381 tgaagatccg catgtcgggc gtcacgttgc acaggatcgt cggttcgacg aagtgcgagt
     1441 cgccctcgcc gaattcgccg ccagccgcaa ccgtggcgcc gtgctcgatg gcatcgtcga
     1501 ccagtgcctt gatgtcctcg agcgccttgc gcgtgatgat cggaccgtgc gtcacactct
     1561 catcgaggcc gtaacccacc ttgtaggtgc ccacgacttt tttcagttct tcgatgaact
     1621 cgtcgtagac cgtgtcctgc acgaggatgc ggttggcgca cacgcaggtc tggcccgagt
     1681 tgcggtactt cgacgctgca gcacccgccg ccgccagctt caggtccgcg tcgtcgaaca
     1741 cgatgaccgg ggcgttgccg ccgagctcca tcgacaggcg cttgagcgta tcggcgcact
     1801 gcttcgtcag gatcttgccc acgcccgtcg agccggtgaa cgtcagcttg cgcacgatcg
     1861 ggttggcgca catctcgtca ccgacagagc gtgagttgtt cgtcggcagg acattgagca
     1921 gtcccgccgg caatccggcc cgatcggcaa gctcggccag cgccgtcatc gacagtggcg
     1981 tctcctgcgg cggcttggcc accatcgaac agccgactgc aagcgcgggc gcgaccttgc
     2041 gggtcagcat cgccgccggg aaattccagg gcgtgatcgc cgcgcacacg ccgaccggtt
     2101 gcttgatcac gatgccgcgc cggtccgtga acggctgcgg caggacgtca ccgtaaacgc
     2161 gcttgccttc ctcggcgaac cactcgatga aagaggcagc atagacgacc tcgcccctgg
     2221 cctcggccag gatcttgccc tgctcgaccg tcatgaggat gccaagatcg tcaacgttcg
     2281 ccatgaccag gtcgtaccac ttcctgagca cgccggagcg ttcgatcgca gactttgccg
     2341 cccaggcttt ttgtgccacc tccgctgcct cgatcgcgcg ccgggtttcc gccgccccga
     2401 cattggccat ctcggcgaca acttcgtgtg tcgcgggatt caggacctgg aaggtcgcgc
     2461 cgtcgtcggc gtcgacccat tcgccgttga tgtaggcctg ggtcctgaga agggagggat
     2521 cactgagcga ccagtctacg ctggctactt cctggagtgc ttgcgacatg attgtgtcct
     2581 gcagaatctg ggtgaaaccg gccgaccatt atgccacgcc gggggttcgt gccg
//
LOCUS       DATDAA010000314        13611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_57733,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000314 DATDAA010000000
VERSION     DATDAA010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13611
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57733"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VIS73_09605"
     CDS             <1..1126
                     /locus_tag="VIS73_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY5763446.1"
                     /translation="DGVAKADYEKVVDHCYLCDLCYMTKCPYTPPHEWNLDFPHLMLR
                     AKAVKHKKGENKLRDKVLTSTDSVGRFAGIPVVAQTINAVNKVGIARKALQGVAGIHA
                     EAWLPEFTSRPLRKRLADLDGQGKAEPAGRTKGRVALFATCYMNRNEPGPGEDLAAVY
                     THNGIPLRLAEKENCCGMPKLELGDLETVEKLKNGNIPVLAKLVDEGWDLTALIPSCV
                     LMFRKELPLLFPDDPDVRKVADAFFDPFEYLVLRHKEGKLKTDFKNQLGKVAYQASCH
                     QRVQNIGPKTKEALSLVPGTEVEVIERCSGHDGTYAVKAEYHQYAMKIVRPVVARIRE
                     FAPDHYGSDCAMAGHHIGHALADGKDPAHPMSLLRKAYGI"
     gene            1151..1726
                     /locus_tag="VIS73_09610"
     CDS             1151..1726
                     /locus_tag="VIS73_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEY5763447.1"
                     /translation="MLRHDDLYSLEQYARLRPEFRARVIEHKKHRRLPLGAHAALYFE
                     DALTMQYQVQEMLRIERIFEPAGIQDELDVYNALVPDGSNWKATFMIEYADAEERRVA
                     LGRLIGVERALWVQVADFDKVRPIANEDLDRETPDKTASVHFVRFEFSPPMIAAMKAG
                     APIRAGIDHPQCTVETEIAGSVRESLVADFS"
     gene            complement(1758..2108)
                     /locus_tag="VIS73_09615"
     CDS             complement(1758..2108)
                     /locus_tag="VIS73_09615"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY5763448.1"
                     /translation="MLPGLFLLALSTVTIFALAPPPAVPQPFHVWDKLQHGIAYAALA
                     WLGGMAFPARHLLLICGLVLHGAAIEVMQATLTSTRHGDPRDWLADCMGVAIGLLALA
                     AWKRWAPRSARSAR"
     gene            complement(2297..3292)
                     /locus_tag="VIS73_09620"
     CDS             complement(2297..3292)
                     /locus_tag="VIS73_09620"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEY5763449.1"
                     /translation="MTTSTIAPRLGAWFCVACLTLAGALPLAAVAETGTPAQQQAARQ
                     ESQPLNNAPVWREVRQEKAHFTNLPQNEAGVLIQSAGNTWRQTRNGPVTVYGGTLILV
                     VAAAILIFYAIKGPLSLHDSPTGRLMERFPPFERLVHWTVAGTFVLLAITGLGILFGK
                     HVLIPVIGHSAFGYLMVAGKTVHNYVGPLFALATVAMIVIWAKDNVWHPLDSKWISKA
                     GGLLTGEHVPSGRFNFGEKSWFWIGVTILGLTVSASGLFVNFPNFEQGRADMQTANLI
                     HSIASIVMMALSFGHIYMGTIGVKGAFQSMKTGYVDETWAREHHEQWYIENKKSG"
     gene            complement(3303..3530)
                     /locus_tag="VIS73_09625"
     CDS             complement(3303..3530)
                     /locus_tag="VIS73_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763450.1"
                     /translation="MNNKNILTAALMGVALAGLSACSEYSQVVEYKPGNYQGKSDTRP
                     WEGGKFAGDKKAWETALTNRAQAQNEYKRVE"
     gene            complement(3536..4153)
                     /gene="fdh3B"
                     /locus_tag="VIS73_09630"
     CDS             complement(3536..4153)
                     /gene="fdh3B"
                     /locus_tag="VIS73_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HEY5763451.1"
                     /translation="MARMKFLCDAERCIECNGCVTACKNEHEVPWGVQRRRVVTINDG
                     VPGERSISVACMHCSDAPCMAVCPTDCFYKTEEGVVLHDKDACIGCGYCFFACPFGAP
                     QFPNGPASFGARGKMDKCTFCAGGPEEDHSKAEFEKYGRNRIAEGKLPSCAEMCATKA
                     LLAGDAAVVADIYRERVLRRGKAGEAWGWLTAYGQPEQPAADKRG"
     gene            complement(4173..7082)
                     /locus_tag="VIS73_09635"
     CDS             complement(4173..7082)
                     /locus_tag="VIS73_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEY5763452.1"
                     /translation="MLVKKAKTRSADSAAPLVGTTQAQSSGILGQTIDRRTFLKRSGV
                     AAGGGVLATQLPYSMIGRAEAAAPPNAKPGVEVKRTVCTHCSVGCAIDAVVENGVWVR
                     QEPVFDSPLSLGGHCAKGASVREHGHGEHRLKYPMKLVNGKYQKISWEQAINEVSEKL
                     MAIRKESGPDATYWVGSSKHNNEQAYLFRKFVSMFGTNNTDHQARICHSTTVAGVANT
                     WGYGAMTNSYNDMQFSKAAMYIGSNAAEAHPVSLVHMLHAKENGCKMIVVDPRFTRTA
                     AKADIYARLRSGSDIPFIWGLLYHIFKNGWEDKAYIKARVYGLDKVREEVMQWTPDKV
                     EDVTGVPEAEVYRIAETMAKNRPSTVVWCMGQTQHTIGNANTRAMCILQLALGNVGVS
                     GGGTNIFRGHDNVQGATDIGPNPDSLPGYFGIAEGSWKHWCSVWDVDYEWIKSQFASE
                     ALMQKPGITVSRWIDGVLEQADAIDQPNNVRAVVFWGHAPNSQSRGLDMVEAMKKLDM
                     LVVVDPYPSASASMAAMVRKDGVYLLPAATQFETSGSVTASNRSIQWREKVIEPLFES
                     RPDHTLMYAFAKKFGYEKELCKHIKIEKDKQGWDEPNVEDILREINKGTWTIGYTGQS
                     PERLKLHMQNMHTFDVKTLRAVGGPCDGDYFGLPWPCFGTPELKHPGTPNLYDTSKHV
                     MEGGGNFRARFGVEKDGVSLLAADGSASKGAELQMGYPEFDHALVKKLGWWDELTDSE
                     KALAEGKNWKTDLSGGIQRVVMKHGCHPYGNAKARAVVWNFPDPVPKHREPLYSPRPD
                     LVDKYPTHDDKKAFWRLPTLYKSVQDSAKNASKDFPLIMTSGRLVEYEGGGEETRSNP
                     WLAELQPEMFAEVNPRDANNAGFRNGDFIWVETPSKGKLKVRAQVTNRVGPGTVFLPF
                     HFSGHWQGKDMLEFYPDGAAPIVRGESVNQATTYGYDAVTMMQETKTTLCRVSKA"
     gene            complement(7093..7299)
                     /locus_tag="VIS73_09640"
     CDS             complement(7093..7299)
                     /locus_tag="VIS73_09640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763453.1"
                     /translation="MKKQTPENLKRRGFLLAAGVGGAGAVAAVAIGVGAETGAQASSA
                     GKADKASGYAETPHISNYYRRARV"
     gene            complement(7327..7953)
                     /locus_tag="VIS73_09645"
     CDS             complement(7327..7953)
                     /locus_tag="VIS73_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEY5763454.1"
                     /translation="MSEMVAELPTDSTLHADEDRARADIYALLSRLFASAPDSAFLET
                     LHRHASERANDDHALGQAWRVLAGAVAGTDAAAIEEEYTRLFLSIGRPEVMLYGSAYL
                     TGFLMEEPLVYLRSDLATLGLDRRLGVGESEDHFSALCDVMRHLILSRTDAPGLARQR
                     DFFLQHMAPWIDDFADALESAPDAVFYPRAAPLLRAFFGVERLAFDML"
     gene            complement(7950..9488)
                     /locus_tag="VIS73_09650"
     CDS             complement(7950..9488)
                     /locus_tag="VIS73_09650"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY5763455.1"
                     /translation="MSADGRMAIIGEADVVTGWAERLAGQREVTALLLAAPASETTAG
                     PTRNFAYRTVSTPTLDGHLGAFTVNWLDGDAPRQGVFDVVLDLSPEPLLKRVELPQGY
                     LAPGRDPLDQALAVIDLLAFDGDFEKPRYVEVNERLCAHSRAKQAGCSNCISVCATEA
                     ISSAGESIHLDPYLCQGCGTCATVCPSGALAYQYPRVADAGLAVKAQIAAYRKAGGVN
                     PRVLFLAEQAGRKALAEMDQSGGSLPDNVIAIELWSADAVGLDLLLGTLSLGAEQLAV
                     LGAGSHDLAPLRQQARLAQLILAGLGYDGERVRIIDCDAPDWLAEITTWTPADAIPTA
                     EFRLLRDKRTTLEFVFDHLIRHAPAASPVIELPEGAPFGSVTVSDACTLCMSCTSACP
                     PGALKAAADTPRLSFLERLCLQCGVCANTCPEQAITLTPQLRVKDRRAERVLREAEIF
                     HCSVCGVAMGAAPTITNMMAKLSGHSMFATPEAKARLTMCADCRVVDLVTHEKSIKAW
                     ELSE"
     gene            complement(9630..10112)
                     /locus_tag="VIS73_09655"
     CDS             complement(9630..10112)
                     /locus_tag="VIS73_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HEY5763456.1"
                     /translation="MTSRRFLDRWSRLKQAAEKAQQIGEALPASGAAAQRAAEEMLVD
                     LGPDSDFRAFLNEEVSEAVRRQAMKTLFADPRFNVIDPLDIYIDDYSISDPIPAEMLA
                     TLNQAKFLWEEKTASDDSDVAAQDCTDVARSSECGGSEPSEAAGSTAAVTTDSPGEKS
                     "
     gene            complement(10109..10573)
                     /locus_tag="VIS73_09660"
     CDS             complement(10109..10573)
                     /locus_tag="VIS73_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HEY5763457.1"
                     /translation="MNRFPIAVIIEKTRLDNRWADERWEVVGVVPAFDDLGEIPRRIF
                     ADETREQFLAARLELELFRDEAPGYHLNLTSPEPRVFIVWRLEDGFARPCNATVSYTE
                     AARFMDASEEVDGTPIPEEIANWLGEFVNTHYKPEPRRKVRRNDPFTIAVKS"
     gene            10714..11517
                     /locus_tag="VIS73_09665"
     CDS             10714..11517
                     /locus_tag="VIS73_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEY5763458.1"
                     /translation="MLRPDITCARRTLTFQIDARNERGELVPTAIAGENPLTLYLDKR
                     EVVTLMTLGAAPEALALGYLRNQHLVARLEDVATVHVDWDVDAVAVTTRPGVEVDMSR
                     IEKRTVTTGCGQGTMFGDLMEEIDSIRLPAKSALDESTLYALLDAVRLHESIYKQAGA
                     VHGCALAAGGEILMFVEDVGRHNAVDAIAGQMWLDGLDGGDKIFYTTGRLTSEMVIKT
                     AQMGIPVLVSRSGLTQMGWEIAQRIGMTMVGRAQGKHYLLFTGAERFRK"
     gene            11514..12125
                     /gene="mobA"
                     /locus_tag="VIS73_09670"
     CDS             11514..12125
                     /gene="mobA"
                     /locus_tag="VIS73_09670"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEY5763459.1"
                     /translation="MTVPEPTVTGVVLAGGLGRRMGGTDKGLQMLRGRTMVEWVIERI
                     VPQVDTLIINANQNLERYASFGHPVVPDRIPDFAGPLAGLHAALSAAATPLVATVPCD
                     SPFLPGDLVRRLREAMVGNEANLAVARTFDQPHPVFCLCRRDLLPHLTGYLEGGGRKI
                     DHWYASLKVVEVAFDDVADAFENINTRDELQRFAAGPPPAGVK"
     gene            complement(12158..12652)
                     /gene="mobB"
                     /locus_tag="VIS73_09675"
     CDS             complement(12158..12652)
                     /gene="mobB"
                     /locus_tag="VIS73_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEY5763460.1"
                     /translation="MKIIGFAGWSGCGKTTLIEQVIGLLRQRGLTVSLIKHAHHNFEI
                     DHAGKDSFRHRQAGCGQVLVTSARRWALMQELGDAPELTLDEALSRLAPCDLVLVEGF
                     KYASIPKIEIYRAALGKPQLFPGDSNFIAVVSDTAVDTSLPQLDINQPSAVAEFIALT
                     ISGG"
     gene            12831..>13611
                     /gene="moaA"
                     /locus_tag="VIS73_09680"
     CDS             12831..>13611
                     /gene="moaA"
                     /locus_tag="VIS73_09680"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY5763461.1"
                     /translation="MPVGVESTSEGPLRDVSNRPLRDLRISVTDRCNFRCVYCMPKSA
                     FGRDHAFLPRDEVLSFEEIERVVRLFVARGGSKVRLTGGEPLLRRNIEELIARLAAIP
                     DVELTLTTNGSLLRRKAAALKSAGLKRVTISLDSIDDTVFRAMNDVDFPLADVLNGID
                     AAAEAGLAPLKINMVVKRGVNDHTLVDMARHFRGSGHILRFIEFMDVGATNGWQLDSV
                     VPSAEVVARIGAQFPLQAIDPNYEGEVAERWRYEDGSGEIGV"
BASE COUNT         2514 a         4275 c         4295 g         2527 t
ORIGIN      
        1 tgacggcgtc gccaaggccg actacgagaa ggtcgtcgac cactgctacc tttgcgatct
       61 ctgctacatg accaagtgtc cgtacacgcc gccgcacgag tggaatctcg attttccgca
      121 cctgatgctg cgggccaagg cggtcaagca caagaagggc gagaacaagc tgcgggacaa
      181 ggtgctgacc agcaccgatt cggtcgggcg cttcgccggg attcccgtgg tcgctcagac
      241 aatcaacgcc gtcaacaagg taggcatcgc gcgcaaggcg ctgcagggtg tcgccggcat
      301 ccacgccgaa gcgtggctgc ccgagttcac cagccggccg ctcaggaagc ggcttgccga
      361 tctcgacgga cagggcaagg ccgagccggc agggcggacc aagggccgcg tcgcgctgtt
      421 tgccacctgc tacatgaacc gcaacgagcc cggccccggt gaggatctcg ccgcggtgta
      481 cacacacaat ggcatcccgc tgcgcctggc cgaaaaggag aactgctgcg gtatgcccaa
      541 gctcgaactc ggcgatctcg aaaccgtcga gaagctcaag aacggcaaca tcccggtgct
      601 cgccaaactg gtcgacgagg gctgggacct gacggcgctg attccctcct gcgtgctgat
      661 gtttcgcaag gagttgccgc tgctgtttcc cgacgacccc gacgtgcgga aggttgccga
      721 cgccttcttc gaccccttcg agtacctcgt actgcgacac aaggaaggca agctgaagac
      781 tgatttcaag aaccagctcg gcaaggtcgc ctatcaggca tcgtgccacc agcgggtgca
      841 gaacatcggc ccgaaaacca aggaagcgct atcgctggtg cccggtaccg aggtcgaggt
      901 catcgagcgt tgctcgggcc acgacggcac ctacgcggtg aaggccgaat accaccagta
      961 cgcgatgaag atcgtccggc cggtggttgc gcgcatccgc gagttcgcgc ccgaccacta
     1021 cggtagcgac tgcgcgatgg ccggccacca tatcggtcat gcgctggccg acggcaagga
     1081 cccggcgcat cccatgagcc tgctgcgcaa ggcctacggg atctgaaaca gaccatcaaa
     1141 gaggaagacc atgctcagac acgacgatct ctacagtctc gaacagtacg cacgcctgcg
     1201 cccggagttc cgcgcacgcg tcatcgagca caagaagcac cgccgcctgc cgctcggcgc
     1261 gcacgcggcg ctctacttcg aagacgcact aacgatgcaa taccaggtgc aggagatgct
     1321 gcgcatcgag cgcatcttcg aacccgcagg tatccaggat gaactcgacg tctacaacgc
     1381 gctcgttccc gacggtagca actggaaggc gacgttcatg atcgagtacg ccgatgccga
     1441 ggaacgccgc gttgcgttgg gccggctgat cggtgtcgag cgcgcgctct gggtgcaggt
     1501 tgccgatttc gacaaggttc gcccgatcgc caatgaagat cttgaccgcg aaacacccga
     1561 caagaccgcg tcggtgcact tcgtacgttt cgaattcagc ccgccgatga tcgcggcaat
     1621 gaaggccggc gcaccgatcc gcgccggtat cgaccatccg caatgcaccg tggagaccga
     1681 gatcgccgga tcggttcgcg agtcgctggt cgcggatttc agctagggcc gccaggcagc
     1741 gccggctgcc gctcgtatca gcgggcgctc cgggccgacc ggggcgccca tcgcttccaa
     1801 gcggcgaggg cgagcagtcc gatggcgaca cccatacagt cggccagcca gtctcgcgga
     1861 tcgccatggc gcgtgctcgt caaggtggcc tgcatgacct cgatcgcggc accgtgcagt
     1921 acgagtccgc agatcagcag cagatgccga gccggaaacg ccattccgcc cagccaagca
     1981 agtgcggcat aggcgatccc gtgttgcagc ttgtcccaga cgtggaaggg ctgcgggact
     2041 gccgggggtg gggcgagcgc gaaaatcgtc accgtcgaca acgccaacag gaacaggccc
     2101 ggaagcaaac ggtctttccg caaactgggg cgcagagttg tatttggcat gggtcagatt
     2161 cgcctgcgcg gcgggatttg gcgctgagga agacgaagcg acatggcgag gcgcagttcc
     2221 ctctgagcag aaagcgcaac gggcggggat tgaaaacccg cccgtcggtc agcgttgagt
     2281 cgagaagtgg tcttggctag cccgatttct tgttctcgat gtaccactgc tcatgatgct
     2341 cgcgcgccca ggtttcatcg acgtaaccgg ttttcatcga ctggaaggcg cctttgacgc
     2401 caatcgtgcc catgtagata tgcccaaaag acagggccat catgacaatt gaagcgatcg
     2461 agtgaatcag gttggcagtc tgcatgtctg cgcggccctg ctcgaaattc gggaagttca
     2521 cgaacagccc ggatgcagac actgtcagcc caaggatcgt gacgccaatc cagaaccagc
     2581 tcttctcgcc gaaattgaat cgccccgacg gtacgtgttc gccggtcaac aacccgccgg
     2641 ccttgctgat ccattttgaa tcgagggggt gccagacgtt gtccttggcc cagatgacga
     2701 tcatcgcaac ggtcgccaac gcgaacagcg ggccgacata gttgtggacg gtcttgccgg
     2761 caaccatcag gtaaccgaac gctgaatgac cgatgaccgg aatcagtacg tgtttgccaa
     2821 agagtattcc cagtccggtg atcgctagta ggacgaaggt tccagcaacg gtccagtgca
     2881 ccagccgctc gaatggcggg aagcgctcca tcagtcgccc agtcggcgaa tcatgcaggc
     2941 tgagtggccc tttgatcgcg tagaagatca ggatcgcagc ggcaacgacc agaatcagag
     3001 tgccgccgta gacggtgacc ggaccattgc gggtttgccg ccacgtgttg ccggccgact
     3061 ggatcaggac cccagcctcg ttctgcggga gattggtgaa atgcgctttt tcctggcgga
     3121 cctctcgcca gaccggggca ttgttcagcg gttggctctc ctggcgcgcg gcctgctgct
     3181 gcgccggcgt tccggtctcg gcaactgcgg ccagcggcag cgctcccgcg agcgtgaggc
     3241 aggccacaca aaaccaggcg ccaagcctgg gtgcaatcgt tgaagtagtc atggcgacct
     3301 cctcactcga cccgcttgta ctcgttctgg gcctgcgcgc gattggtcag ggcagtctcc
     3361 caggccttct tgtcacccgc gaacttgccg ccctcccacg gtcgcgtatc ggacttgccc
     3421 tgatagttgc ccggtttgta ctcgacgacc tgagagtact cgctgcaagc cgagagtccg
     3481 gcaagcgcaa cacccatcag cgcggcagtc agaatgttct tgttgttcat ggcgatcacc
     3541 cccgtttgtc ggctgcaggt tgttccggtt ggccgtaagc cgtcagccag ccccaggcct
     3601 cgccggcctt gccgcgtcgc aatactcgct cgcgatagat gtcggcgacg accgcggcgt
     3661 ccccggcgag caaggccttg gtcgcgcaca tttcggcaca cgacggcaac ttgccctcgg
     3721 cgattcgatt gcggccgtac ttctcgaact cggccttcga gtggtcctct tcggggccgc
     3781 cagcgcagaa ggtacatttg tccatctttc cgcgggcgcc gaacgaagcg ggaccgttgg
     3841 gaaactgggg cgcaccgaac gggcaggcga agaagcagta gccacagccg atgcaggcgt
     3901 ccttgtcgtg gaggacgaca ccctcttcgg tcttgtaaaa acaatcggtc gggcagaccg
     3961 ccatgcaggg cgcgtcagaa caatgcatgc aggccaccga aatcgatcgc tcgccgggaa
     4021 cgccgtcgtt gatggtgacg acgcgtcggc gctggacacc ccaagggacc tcatgctcgt
     4081 tcttgcaggc cgtgacgcag ccattgcact cgatacagcg ctccgcgtcg caaagaaatt
     4141 tcatgcgtgc cattatttgt ctcctcgctc gctcaggcct tggacacgcg acagagcgtg
     4201 gtcttggtct cttgcatcat cgtcaccgcg tcgtaaccgt aggtagtcgc ctgattgacc
     4261 gactccccgc gcacgatcgg cgctgcgccg tccgggtaga actccagcat gtccttgcct
     4321 tgccagtgcc cggaaaaatg gaatggcagg aacaccgttc cggggccgac ccggttcgtg
     4381 acctgcgcgc gaaccttgag cttgcccttt gacggagttt cgacccagat gaagtcgccg
     4441 ttgcggaagc cggcattgtt cgcatcgcgc ggattcacct cggcaaacat ctcgggctga
     4501 agttcggcca gccacgggtt ggaacgcgtt tcctccccgc cgccttcgta ctcgacgagc
     4561 cgccccgagg tcatgatcag cgggaaatct ttggacgcgt tcttcgcgct gtcctgcact
     4621 gacttgtaga gcgtcggcaa gcgccagaaa gccttcttgt cgtcatgcgt cgggtacttg
     4681 tcgacgagat cgggtcgggg tgagtacagc ggttcccggt gcttcggcac cgggtccggg
     4741 aagttccaga ccaccgcgcg cgccttggcg ttgccgtagg ggtggcaacc gtgtttcatg
     4801 accacgcgct ggatcccgcc cgagagatca gtcttccagt tcttgccctc agcgagggcc
     4861 ttctcgctgt ccgtcagttc atcccaccag cccaatttct tgaccagcgc gtgatcgaac
     4921 tcgggatagc ccatttgcag ttccgcccct ttggacgccg aaccgtccgc cgcaagaagc
     4981 gatacgccat ccttctcgac gccgaaacgg gcgcggaagt tgccgccgcc ttccataaca
     5041 tgcttggaag tgtcgtacag attcggcgtg cccggatgtt tgagttccgg cgtgccgaaa
     5101 cacggccacg gcagtccgaa gtaatcgccg tcgcaggggc cgcccacggc gcgcagggtc
     5161 ttcacgtcga aggtgtgcat gttctgcatg tgtagcttca gacgctccgg cgactggccg
     5221 gtatagccga tggtccaagt gcccttgttg atctcgcgca ggatgtcctc gacgttgggt
     5281 tcgtcccagc cctgcttgtc cttctcgatc ttgatgtgct tgcagagctc cttctcgtag
     5341 ccgaacttct tggcgaaggc gtacatcagt gtgtgatcgg gccgtgactc gaacagcggc
     5401 tcgatgacct tttcacgcca ctgaatcgaa cggttcgatg cagtcaccga gcccgaggtc
     5461 tcgaactgcg tcgccgccgg cagcagatag accccgtcct ttctgaccat ggccgccatc
     5521 gaggccgatg ccgacgggta ggggtcgacg acgaccaaca tgtccagctt cttcattgcc
     5581 tcgaccatgt ccaggccgcg cgactggcta ttcggcgcgt gtccccagaa caccacggcg
     5641 cgaacattgt tgggctggtc gattgcgtcg gcctgctcga ggacaccgtc aatccagcgc
     5701 gaaaccgtga tgcccggttt ctgcatcagc gcctcggagg caaactggct cttgatccac
     5761 tcgtagtcaa cgtcccacac cgagcaccag tgtttccagg agccctcggc gatcccgaaa
     5821 taacccggca gcgaatccgg gttggggccg atgtcggtgg cgccctgcac gttgtcgtgg
     5881 ccgcggaaaa tgttggtgcc accccccgat acgccgacgt ttccgagcgc cagctgcagg
     5941 atgcacatgg cgcgcgtatt ggcgttgccg atcgtgtgct gggtctgacc catgcaccaa
     6001 acgacggtcg aagggcggtt cttggccatg gtctcggcga tgcgatagac ctcggcctca
     6061 ggcacgccgg tgacatcctc gaccttgtcc ggcgtccact gcatgacctc ttcacggacc
     6121 ttgtcgaggc cgtagacacg ggccttgata taggccttgt cttcccagcc gttcttgaag
     6181 atgtgataga gcaggcccca gatgaaggga atgtcggaac ccgagcgcag ccgtgcatag
     6241 atgtccgcct tggccgccgt acgcgtgaag cgcgggtcga caacgatcat cttgcagccg
     6301 ttctccttgg cgtgaagcat atgcaccagc gataccgggt gtgcttcggc ggcgttcgag
     6361 ccgatgtaca tcgccgcctt ggagaactgc atgtcgttat aggagttggt catcgccccg
     6421 tatccccaag tattggcgac gccggcgacg gtagtcgagt ggcagatacg ggcctggtgg
     6481 tcggtgttgt tggtgccgaa cattgatacg aacttgcgga acaggtaggc ctgttcgttg
     6541 ttgtgcttcg acgacccgac ccagtaggtt gcgtccgggc cgctttcctt acggatcgcc
     6601 atcagctttt cggagacctc gttgatcgcc tgctcccaac tgatcttctg gtacttgccg
     6661 ttaaccagct tcattggata cttcagacga tgctcgccat ggccgtgctc acgaaccgag
     6721 gcgcccttgg cgcaatggcc gccaagactg agtggcgagt cgaaaactgg ttcctgccgg
     6781 acccagacgc cgttctcgac gaccgcgtcg atggcgcaac cgaccgagca atgcgtgcag
     6841 acagttcgtt tcacttcgac gccgggcttg gcattcggcg gcgcggcggc ttcggcgcgg
     6901 ccgatcatgc tgtagggaag ttgcgtggcg agtactccgc cgccggccgc taccccggaa
     6961 cgcttaagga aggtccgtcg gtctatggtt tggccgagaa tgccggagga ctgtgcctga
     7021 gtagtgccga ccagcggtgc cgcggaatcg gccgaacgag tctttgcctt cttgacgagc
     7081 atgatggcct cctcaaaccc gcgcgcggcg gtagtagttg ctgatgtgcg gcgtttcggc
     7141 gtagccgctg gccttgtcgg ccttgcccgc actcgatgcc tgcgcgccgg tttccgcgcc
     7201 tacaccgatc gcgacggcgg ctacggcgcc agcgccgccg acaccggcgg cgaggaggaa
     7261 accacgtcgc ttcaggttct cgggggtttg cttcttcatg atgtctcctc ctcgttctgg
     7321 ggcctgctag agcatgtcga acgccaggcg ttcgacacca aaaaaggcgc gcaacaaggg
     7381 cgctgcgcgc gggtagaaaa cggcgtccgg ggctgactca agagcgtcag caaagtcgtc
     7441 gatccagggg gccatgtgct gcaggaagaa atcccgctgc cgcgcaagtc ctggggcatc
     7501 ggttctgctc aagatcagat ggcgcatgac atcgcacagg gccgagaagt gatcttcgga
     7561 ttcgccgacg ccgagacggc ggtcaaggcc gagcgtggcc agatcgctgc gcagataaac
     7621 caggggctct tccatcagaa aaccggtcag atacgccgag ccatagagca tgacctcggg
     7681 ccggccaatg ctcaggaaca ggcgggtgta ttcctcttcg atggccgccg catcggtgcc
     7741 ggccactgcg ccggccagca ctcgccacgc ctgacccagc gcgtggtcat cgttggcccg
     7801 ctcgctggca tggcgatgca gtgtttccag aaaggccgaa tcgggtgcgc tggcgaagag
     7861 ccgcgagagc agcgcgtaga tgtcggcacg ggcacgatct tcgtcggcgt gcagcgtcga
     7921 atccgtcggc agctcggcga ccatctcgct cattcggaaa gctcccatgc cttgatgctc
     7981 ttctcgtggg tgaccaggtc aacgacccgg caatcggcac acattgtcag acgcgccttc
     8041 gcttcgggtg tcgcaaacat gctgtgcccc gacagcttgg ccatcatgtt ggtgatcgtc
     8101 ggcgctgcgc ccatcgccac gccgcagacg ctgcagtgga agatctcggc ctcgcgcagc
     8161 acgcgttcgg cgcggcggtc cttgacccgt agctgcggcg tcagcgtgat tgcctgctcc
     8221 ggacaggtat tggcgcagac gccgcattgc agacacaggc gttcaaggaa ggacaggcgc
     8281 ggggtatcgg cagctgcctt gagtgcgccg ggtgggcagg cgctggtgca ggacatgcac
     8341 agcgtgcatg catcgctgac cgtaacgctg ccgaagggcg caccttcggg aagttcgatc
     8401 accggcgatg cggccggcgc gtgccgaatc agatggtcga agacgaattc gagcgtcgtt
     8461 cgcttgtcac gcaggaggcg gaactcggcc gtcggaatgg catcggcggg cgtccaggtg
     8521 gttatttcgg caagccagtc gggcgcatcg cagtcgatga tccgcacgcg ttcgccgtca
     8581 tagccgaggc cggcaagaat cagctgagcg agtctggcct gctggcgcaa cggtgccaga
     8641 tcgtgcgagc ccgccccgag cacggcaagc tgctcggcgc cgagcgacag ggtgccgagc
     8701 agaagatcca gcccgacggc atcggcactc cacagttcaa tcgcgatcac gttgtcgggc
     8761 aaggaaccgc cggattggtc catctcggca agtgctttcc gacccgcttg ttcggcgagg
     8821 aacagcacgc ggggattgac gccgccggcc ttccgatacg cggcgatctg ggccttgacc
     8881 gcgagaccgg cgtcggcgac gcgcggatac tgatacgcca gtgcgcccga cgggcagacg
     8941 gtggcgcagg tgccgcagcc ctggcacaga taggggtcga ggtggatcga ctcaccggcg
     9001 ctggaaatcg cttcggtcgc gcacaccgag atgcaattgc tgcagcccgc ttgctttgcg
     9061 cggctgtgcg cacacaggcg ctcgttgact tcgacgtaac gcggtttctc gaagtcgcca
     9121 tcaaaggcca gcaggtcgat gactgccagg gcctggtcga gggggtcgcg cccgggcgcc
     9181 aaatagccct gcggcaattc gactcgcttc agcagcggct cgggcgagag atcgagcacg
     9241 acatcgaaga caccctgccg cggggcgtcg ccgtcgagcc aattgaccgt gaaggcgcca
     9301 agatggccgt cgagcgttgg cgtgctgacg gttcggtatg cgaagtttcg cgtcggtccg
     9361 gccgtggttt cggatgccgg cgcggccagc aaaagggcgg tcacttcgcg ctgcccggcg
     9421 agccgttcgg cccaacccgt cacgacatcc gcttcgccga tgatggccat ccgcccatcg
     9481 gcggacatgc ttaccgccgg tactggctcg ggggctggca gcgcgctcag cgctgcacgc
     9541 gccgcaattg ccggcacagc gtcagagggt cttgcagaat tggaactggt tggactcatg
     9601 acagagatcc gcctcgctcg cactgcgttt caggattttt cacccgggga gtcagtggtg
     9661 acagccgcag tggaccctgc ggcttcagat ggctccgatc caccacactc tgaactcctc
     9721 gctacatctg tgcaatcctg cgccgcaaca tcgctgtcat cagatgccgt tttctcttcc
     9781 cacaagaact tggcctggtt gagcgttgcc aacatctcgg cggggatcgg gtcgctgatt
     9841 gagtagtcgt cgatgtaaat gtctagcgga tcgatgacat tgaatcgcgg gtcggcgaac
     9901 agggtcttca tcgcctgccg ccgcacggct tcgctgacct cttcgttgag gaaggcgcga
     9961 aagtcactgt cgggaccaag gtcgaccagc atctcttcgg ctgctcgttg tgccgcggcg
    10021 ccgcttgccg gaagcgcttc gccaatctgc tgcgccttct cggcggcttg ctttagccgc
    10081 gaccaacgat cgaggaagcg acggctggtc atgatttgac cgcaatcgtg aaagggtcat
    10141 tacgcctgac cttgcggcgc ggctccggct tgtaatgcgt gttgacgaac tcgccgagcc
    10201 agttggcgat ttcttcgggg ataggcgttc cgtcgacctc ctcgctggcg tccatgaagc
    10261 gcgccgcctc ggtgtagctg acggtcgcat tgcaggggcg ggcgaagccg tcctcgagcc
    10321 gccagacgat gaacacacgg ggctcgggcg aggtcaggtt gaggtggtag cccggggcct
    10381 cgtcgcggaa aagctcgagt tccaggcgcg ccgcgaggaa ctgctcgcgc gtctcgtcgg
    10441 caaagatccg gcgcgggatt tctccgagat catcgaaggc tggaacgacg ccgacgactt
    10501 cccagcgctc gtcggcccag cgattatcga ggcgggtttt ttcgatgatc acggcgatcg
    10561 gaaatcgatt catgaggata gcccggggta gtcatgacgg cgtgaacact tcagcatagt
    10621 cgaatcagac tacacctgtc ggcgagcccc gtccgcgtgt ccatgcgatt ttgagactag
    10681 aatttcctcc cgcaattgtt ggctgatcga ctgatgctcc gccctgatat cacctgcgcc
    10741 aggcgcacgc tgacctttca gattgacgcg cgcaacgagc gcggcgagct ggtaccaacg
    10801 gccatcgctg gcgagaaccc gctgacgctc tacctcgaca agcgcgaggt cgtcaccctg
    10861 atgacgttgg gcgcagcccc cgaggcccta gcattgggat acctgcgcaa tcagcatctg
    10921 gtcgcccgac tcgaagatgt ggcgaccgtg catgtcgact gggacgtcga cgcggtggcc
    10981 gtcactacgc ggcccggcgt cgaggtcgac atgagccgga tcgaaaagcg gacggtaacc
    11041 accggctgtg gccagggcac gatgttcggc gacctgatgg aagagatcga ttcgatccgc
    11101 ctgcctgcca aaagcgcact cgacgaatcg acgctctacg cgctgcttga tgccgtgcgc
    11161 cttcacgaat cgatctacaa gcaggccggg gctgtgcatg gctgcgcact tgccgcgggc
    11221 ggcgagatcc tgatgtttgt cgaagacgtc ggccgccaca acgcggtcga tgcgattgcc
    11281 ggccagatgt ggctcgacgg actcgatggc ggcgacaaga tcttctacac caccgggcgg
    11341 ctcacctcgg aaatggtcat caagaccgcg cagatgggaa ttcccgtgct cgtttcgcgg
    11401 tcggggctga cgcaaatggg ctgggaaatc gcgcagcgga tcggcatgac gatggtcggc
    11461 cgtgcgcagg gcaagcatta cctgctgttt accggcgccg agcgttttag gaaatgactg
    11521 tgcccgagcc aacggtgacc ggcgttgtcc tcgcgggcgg actcggcagg cgcatgggcg
    11581 gcaccgacaa gggccttcag atgctgcgcg ggcgaaccat ggtcgaatgg gtgatcgagc
    11641 ggatcgtgcc gcaggtcgac acgctgatca tcaacgcaaa ccagaatctc gagcgctacg
    11701 cgagcttcgg acacccggtg gttcccgacc gcatcccgga tttcgccggc ccgctcgccg
    11761 gtctacacgc tgccctgtcg gcggcggcga ccccgctggt cgcgaccgtg ccctgcgact
    11821 cgccgtttct gcccggcgat ctggtgcggc gcctgcgcga agcaatggtg ggcaatgaag
    11881 cgaacctggc ggttgcgcgc accttcgacc agccgcaccc ggtgttctgt ctgtgccgcc
    11941 gtgatctcct gccgcatctg accggctatc tcgaaggcgg tggccgcaag atcgaccact
    12001 ggtacgccag cctcaaggtg gtggaagtcg cattcgatga cgtcgccgac gccttcgaga
    12061 acatcaacac gcgcgacgag ctgcagcgtt tcgcagccgg cccgcctcca gccggcgtca
    12121 agtaggcgcg actcgccggc aagcgattcc ggtcatgtca gccgccgcta atggtcagcg
    12181 caatgaactc ggctaccgcc gagggctgat tgatgtccag ttgcggcagg ctggtatcga
    12241 cggcggtgtc gctgaccacc gcaatgaagt tcgaatcgcc cggaaacagt tgcggcttgc
    12301 ccagcgcggc gcggtagatt tcaatcttcg gaatcgaggc gtacttgaag ccctcgacca
    12361 gcaccagatc gcagggcgcc aggcgcgaca aggcctcgtc cagcgtcagt tccggcgcat
    12421 cgcccaactc ctgcatcagt gcccagcgcc tggccgaggt caccaatacc tgcccacaac
    12481 ccgcctggcg gtgacggaac gagtccttgc ccgcgtggtc aatctcgaag ttgtgatgag
    12541 catgcttgat caaggaaacc gtcaggccgc gctgacgcag caaaccgatt acctgttcga
    12601 tcaatgtggt cttgccgcaa cccgaccaac ccgcaaagcc aatgatcttc atcctgatcc
    12661 cagccagtgc cgcggactgc cgaggtccgc agcgatattt cgttatcggc aaactgccgg
    12721 agacgcgagt ttcgtcgcta gcggaaaggc aagtcaattg aatcggcaat cgatcgatgg
    12781 ctaagcccgt cattcccatt cttgaccatc gtctcgtcgc cgcaacgccg atgccagtcg
    12841 gtgtcgaatc gaccagcgag ggaccgctgc gcgacgtcag caatcgaccg ctgcgcgact
    12901 tgcgcatatc ggtcaccgac cgctgcaact tccgttgcgt ctactgcatg ccgaagtccg
    12961 cgttcgggcg cgaccatgcc tttttgccgc gcgacgaagt gctgagcttc gaggaaatcg
    13021 aacgggtcgt acggctgttc gtcgcgcgcg gcggcagcaa ggtccgactg accggcggcg
    13081 agcctctgct gcggcggaac atcgaggagc tgatcgcacg gctggcggcg ataccggatg
    13141 tcgagctgac gctgaccacc aacggctcgc tgctgcggcg caaggcggcc gccctgaagt
    13201 cggctgggct gaaacgcgtc accatcagcc tcgactcgat cgacgacacg gtcttcagag
    13261 ccatgaacga cgtcgacttt ccgctcgccg acgtactcaa cggcatcgat gccgcggccg
    13321 aggcgggtct ggcgccgctg aagatcaaca tggtcgtcaa gcgcggcgtc aacgaccaca
    13381 cgctggtgga catggcgcgc catttccgtg gctccggtca tatcctccgt ttcatcgagt
    13441 tcatggacgt gggcgccacc aacggctggc agctggattc agttgttccg tcggcagagg
    13501 tggtcgcccg tatcggcgcg cagtttccgc tccaggcgat cgaccccaac tacgagggcg
    13561 aggtcgccga gcgctggcgc tatgaagacg gcagcggaga gatcggtgtc a
//
LOCUS       DATDAA010000315         4204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_338071,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000315 DATDAA010000000
VERSION     DATDAA010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338071"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="VIS73_09685"
     CDS             complement(<1..154)
                     /locus_tag="VIS73_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HEY5763462.1"
                     /translation="MAISVFDLFRIGIGPSSSHTVGPMRAARQFALRLERDGHLASTA
                     GLRIELF"
     gene            complement(347..1375)
                     /locus_tag="VIS73_09690"
     CDS             complement(347..1375)
                     /locus_tag="VIS73_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY5763463.1"
                     /translation="MSRKQSRSVSLALQGGGAHGAFTWGVLDALLDEDFSFDGVTGTS
                     AGAMNAVCLAQGLMAGGRDGAREALRNFWTAVAATSPFQNMFDENGKASATAKMMAQW
                     ANYLTPEQINPMDINPLRDIIEQQIDFPALRKRSPIRLFIAATHANSGKLRIFHNDSL
                     SADALLASACLPTIHRTIYINRQPFWDGGYSANPAVFPLVYDCDASDILLVLLTPLRY
                     QEAPVTADHIRERLGELAFNSTFLREMRMFAHMREYVTRSRLPDWLVKRLPHGRLGWR
                     VADLRFHAISAEKFLGAMPVESKGNMSMDFFSTLFDEGRAVARRWLRRNRHEVGRNQT
                     LNLTKVFY"
     gene            1743..2366
                     /locus_tag="VIS73_09695"
     CDS             1743..2366
                     /locus_tag="VIS73_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763464.1"
                     /translation="MEVIHKITGQIASVGLPLFAVTLTAVPCANTPVLLILHWHGFRR
                     SGDSGEAAEPPVSVPGSALQLNERWDEMARLDEAMLDAAWQCGAWELDREERRACNTV
                     GADEREAAACLQAFGEPPLAGTSETLVADAPDQREMLRLGAAVGYVRWQFRPVRGGLW
                     GPSPEDCSLAADGSRDPPCPVKPLATVGPRVSRTRYQLGRCDRILLP"
     gene            2630..2950
                     /locus_tag="VIS73_09700"
     CDS             2630..2950
                     /locus_tag="VIS73_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY5763465.1"
                     /translation="MQIRPLYDRIIVKRLESQRTTAAGIVIPDTAAEKPEQGEVVAVG
                     DGRLLQDGSVRPLQVKPGERVLFGKYAGQTVRVDGEELLVMREEDVLGVIEGAAAGSF
                     KKAA"
     gene            2987..>4204
                     /gene="groL"
                     /locus_tag="VIS73_09705"
     CDS             2987..>4204
                     /gene="groL"
                     /locus_tag="VIS73_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764708.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY5763466.1"
                     /translation="MAAKDVKFHDSARARIVRGVNVLADAVKVTLGPKGRNVVLERSF
                     GSPLITKDGVTVAKEIELKDKFENMGAQMVKQVASKTADVAGDGTTTATVLAQSIVSE
                     GMKYVAAGMNPMDLKRGIDKAVQAAVDELAKQSKPCATNKEIAQVGSISANSDEDIGQ
                     IIARAMDKVGKEGVITVEDGKSLDNELEVVEGMQFDRGYISPYFINNPDKQKAVLDDP
                     LILLFDKKISSIRDLLPVLEEVAKAGKPLLIIAEDVEGEALATLVVNSMRGVLKVAAV
                     KAPGFGDRRKAMLEDIAILTGGTVISEETGLQLEKVTLKDLGRAKRVEVQKENTVVID
                     GAGDAAKIEARVAAIRRQIDDATSDYDREKLQERLAKLAGGVAEVRVGAATEVEMKEK
                     KSRVEDALHATRAA"
BASE COUNT          822 a         1247 c         1394 g          741 t
ORIGIN      
        1 cgaacaattc gatgcgcaga ccagccgttg acgcgaggtg gccgtcgcgt tcgaggcgca
       61 acgcgaactg ccgcgcagcg cgcatcggcc cgaccgtgtg tgaactagac ggaccgatgc
      121 caatgcggaa aagatcgaac acgctgatcg ccatcgctgt ctcctgcgaa tccgtgggct
      181 gccctgcgga gcgtcccggg agaaaagcat acgccgggac gcgcggcgtg cgggtcgtca
      241 ctgcgctagc gtcctctatc aagactcgcc gattcaccgt tgccgcgacc gtctttcggc
      301 cggaataggc ccggcagtgt tccgtttgcc gacggtcgga tggcgcttag tagaagacct
      361 tggtgaggtt cagcgtctga ttgcgcccga cctcatggcg gttgcgccgc agccaacggc
      421 gcgcgacggc gcgtccctcg tcgaacagag tcgaaaagaa atccatgctc atgttgccct
      481 tgctctcgac gggcatggcg ccaagaaatt tctccgcact gatcgcgtga aaccgcaggt
      541 cggcgacgcg ccagcccagc cgaccgtgtg gcaagcgctt caccagccag tccggaagtc
      601 ggcttcgggt cacgtattcg cgcatgtgcg caaacatccg catctcccgc aggaaggtcg
      661 aattgaaggc gagttcgccg aggcgctcac ggatgtgatc ggccgtcacc ggcgcctcct
      721 ggtatcgcag gggcgtcagc agcacgagca ggatgtcgga tgcgtcgcag tcgtagacca
      781 gcggaaacac cgcgggattg gcgctgtagc caccgtccca gaacggctgc cggttaatgt
      841 agatggtgcg gtggatcgtc ggcaggcagg ccgacgccag cagggcgtct gcggaaagcg
      901 agtcgttgtg aaaaatgcgc agcttgccgg aattggcgtg ggttgccgcg atgaaaagac
      961 gaatcgggct gcgcttgcgc agggcgggga agtcgatttg ctgctcgatg atgtcgcgca
     1021 gcggattgat atccatcgga ttgatctgct ccggcgtcag gtaattcgcc cactgagcca
     1081 tcatctttgc cgtggccgat gccttgccgt tctcgtcgaa catattctgg aatggcgagg
     1141 tcgcggcaac cgccgtccag aaattgcgca atgcctcgcg ggcaccatcc cggccacccg
     1201 ccatcaggcc ctgcgcgagg cacacggcgt tcatggcgcc ggcgctggtg ccggtgacac
     1261 catcgaaaga aaagtcctcg tcgaggaggg cgtccagtac gccccaggta aaggcgccgt
     1321 gagcgcctcc gccttgcagc gccaggctga cgctacgaga ttgcttacgg ctcatggggt
     1381 ttcgtggctc ggaccaaatc gattaaccag ttctgagagg gtgggcggca aacagtcaca
     1441 gtctttgatt gaaacgctgg cgccgccggt tgcggccacg gccctgcgcc aagctcgcgg
     1501 gcaagggtac ttcagaaccg gaccttctca tttgaggcga cactatcacg gtagcgtgtg
     1561 catcactccc agattgtctg atcgattcgg cgataaccgc caggcggctg gtcatggctg
     1621 agcgggaacc gatcttcagg ttgccttgat cgattaggac aacatcgcga gacgaccagt
     1681 ttgcgacggc cgatggtttt gctaaatttt ctccgtgatc gcgtgctgat ccggaggtct
     1741 ccgtggaagt gatccataag attaccgggc agatcgccag cgtcggtctg ccgctcttcg
     1801 ccgtgacgct tacggccgtt ccatgtgcca acacgccggt actgctgatt cttcactggc
     1861 acggtttccg tcgctcgggc gattccggcg aagcggcgga gccgccggtg tcagttcccg
     1921 gctccgctct gcaactcaac gaacgatggg acgaaatggc tcggcttgac gaagccatgc
     1981 tcgacgccgc gtggcaatgc ggtgcctggg aactcgatcg tgaagaacga cgggcttgca
     2041 acaccgtcgg cgccgatgag cgcgaggcgg cggcttgcct gcaggctttc ggtgagcctc
     2101 ctctagctgg gacttccgaa acgctggtcg ccgatgcacc tgaccagcgc gagatgctgc
     2161 gtctcggggc cgcggtcggt tatgtccgct ggcagtttcg cccggtccgc ggtgggcttt
     2221 ggggtccctc acccgaggat tgctcgctgg ctgccgacgg cagccgcgat ccgccttgtc
     2281 cggtcaagcc attggcaacg gtcggcccgc gggtgtcacg gacacgttat caattggggc
     2341 gctgcgaccg gatcttgctc ccgtagcggc agccggaagc cctgctcggc caagccgggc
     2401 aacccgcacg cgtggacttc cggtggctgg catttcggtg accaacagcc ggccgatgtt
     2461 catcgccagg ccatctcgcc tagggtcttg aaaccccaaa aaccgaccct atttcttcac
     2521 tcgccgacct tgatcggcac cgtcgactgc ttcgcgcccg gctccagcgg ggcgcggcgc
     2581 ggcgtaggtc ttcaattgac ttgatttgct atcgcacagg agatcaatga tgcagattcg
     2641 tcccctttac gaccggatca tcgtcaagcg cctggagagc cagcgcacga ctgctgccgg
     2701 gattgtcatt cccgataccg cagcagagaa gccggagcag ggagaggttg ttgccgtcgg
     2761 cgatggccgc ctcctgcagg acggcagcgt gcggccattg caggtgaagc cgggcgagcg
     2821 agtcctcttt ggcaagtacg ccggccagac cgtcagggtt gatggcgaag agttgctcgt
     2881 catgcgcgag gaagacgtgc ttggcgtgat cgaaggcgcg gctgccggct cattcaagaa
     2941 agccgcgtaa gcccaacaga cgacagagca gagaaggaga taagtcatgg cagcaaagga
     3001 tgtgaagttt cacgacagtg cacgggcccg catcgtgcgc ggcgtcaacg tcctggccga
     3061 cgcggtcaag gtgacgctgg gcccgaaggg ccgcaatgtg gtgctcgagc gaagctttgg
     3121 ctcgcccctg atcaccaagg acggcgtgac ggtcgccaag gagatcgagc taaaggataa
     3181 gttcgagaac atgggcgcgc agatggtgaa gcaggtggcc tcgaaaacgg ccgacgtggc
     3241 cggtgacggc acgaccaccg cgactgtgct ggcccagtcg atcgtcagcg aaggcatgaa
     3301 gtatgttgcc gccgggatga acccgatgga cctcaagcgc ggcattgaca aggcggtgca
     3361 ggctgccgtc gacgagctcg cgaagcaatc caagccctgc gcgaccaaca aggagattgc
     3421 ccaggtcggc tcgatctcgg cgaactcgga cgaggacatc ggtcagatca tcgccagggc
     3481 aatggacaag gtcggcaagg aaggcgtgat caccgtcgag gacggtaagt cgctcgacaa
     3541 cgagctcgaa gttgtcgaag gcatgcagtt tgaccgtggc tacatcagcc catacttcat
     3601 caacaacccg gacaagcaga aggcggtact cgacgacccg ctgatcctgc tcttcgacaa
     3661 gaagatcagc agcattcgcg acctgctgcc ggtgctcgaa gaagtcgcca aggccggcaa
     3721 gccgctgctg atcatcgccg aggacgtaga gggcgaagcg ctggcgacgc tggtggtcaa
     3781 cagcatgcgc ggcgtgctca aggtggctgc agtcaaggcg ccgggtttcg gcgatcgtcg
     3841 caaagccatg ctcgaggaca tcgcgattct tacgggtggg acggtgattt cggaagagac
     3901 aggcctgcag cttgaaaagg tgaccctgaa ggatctaggc cgcgccaagc gtgtcgaggt
     3961 gcagaaggag aacaccgtgg tgatcgacgg cgctggcgat gccgcgaaga tcgaggcgcg
     4021 cgttgcggcg atccgtcgtc agatcgacga cgcgaccagc gactacgatc gcgagaagct
     4081 gcaggagagg cttgccaagc tcgcaggcgg cgtcgccgag gtcagggtcg gagcggcgac
     4141 cgaggtcgag atgaaggaga agaagtcccg cgtcgaggac gcgctgcacg cgacgcgcgc
     4201 ggcc
//
LOCUS       DATDAA010000316         5199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_23182,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000316 DATDAA010000000
VERSION     DATDAA010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23182"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2277)
                     /locus_tag="VIS73_09710"
     CDS             complement(<1..2277)
                     /locus_tag="VIS73_09710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5763467.1"
                     /translation="MPMDEQQPGRKLEPQEASPRANARVNQPALAQQAVFDNAAVGIA
                     FTDHRQFVRCNRRWCEMFGYTAEELTGCPAKMLYPTDDAYEALGAAASPALAEGECFQ
                     TEVEMRRKDGDLFWVRIWGRAVDPSRPTGGTVWIAEDCTAEREARSREREVRRQLEAI
                     FETAPIGVAVIANRVLLQCNRRFEEIFGYASGEMLGYTTRLWYASDEDYAGINAITDE
                     AFARGSTHRREQVLRRKDGQPFWCRLTGRCFDPREPQAGAVFLLEDVSERRTREESLR
                     AALDEQQSIFHNAAVGILLVRNRVVQRCNRRLESIFGYEPGGLNGRSTAVLFASHADF
                     EVIGRDAYAALEQDEIYADEWQVKRADGSLFWVRATGHAVTDVEEAGGIGVIWIIDDV
                     TDRHDMQEQLLAAQRDLEQRVAERTAALAEANALLKAEVFDRIKAEERVWQIAHHDTL
                     TGLPNRSLFTDRAQQALVQAGRSGQRVAVLFIDLDRFKQINDSLGHEVGDIFLKGVAA
                     RIAAAVRQSDTVARLGGDEFVVLAQQVNGVEDVRRIAGKIIGGLREAVAAGSHELHPS
                     ASIGIAIYPEDGSDLAALLRAADTAMYAAKAGGRNDYRFFSGEMADETARLFDLEQAL
                     RRAVETDGLRVVYEPVVDIGRRRVCAIEAKLRWYDAERGEVPPESFRPVAEESGLIIP
                     IGERLLLRAATQAAAWARQGYPQVPVVVDLSPRQFWHKSLVSKLRDLLAETSLPPARL
                     ELDIDENALMRDAATSQEV"
     gene            complement(2344..3219)
                     /locus_tag="VIS73_09715"
     CDS             complement(2344..3219)
                     /locus_tag="VIS73_09715"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5763468.1"
                     /translation="MKIGFIGLGDMGRHMAGHLLAAGHELGVWARRREAAEELVGGGA
                     GWFNSAAALAGRSDVVITMVTKDEDVREIALGPQGLREGFARDSIHLDMSTIAPSTAR
                     DLAASYAERGVHFIDAPVSGGPTGAAAASLSIMAGGDDGPVERVMPIFEALGKTIVHI
                     GPVGAGQVAKICNQMIMVATIEACAEAVSLWHATGVDGEAGLKALSGGSAASRALEVF
                     GGRMVRGDFAAGVQARLHHKDYAIIASEARAMGVAMPLTAAVAEQLNALMALGFGRDD
                     TSSLLRVLEFPKRES"
     gene            complement(3230..4108)
                     /locus_tag="VIS73_09720"
     CDS             complement(3230..4108)
                     /locus_tag="VIS73_09720"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5763469.1"
                     /translation="MRIGFIGLGLMGRPMALNLIKGGHAVSVWARRVESTKPLADAGA
                     KVMASPKALAAGVEVVVSMVADGPDVEQVMLGKDGVIEGGNKGLIAIDMSTIQPSTAK
                     QVNAELGKKGIEFLDAPVSGGDVGAINGTLSIMVGGPKAAFDKAKPVFECMGKNIVYI
                     GETGAGQVTKAANNLVVAMTVIAVSEAFCLARKSGVDPAKVREALLGGFANSKVLDLH
                     GQRLLDRNFKPGFKAWMHQKDLHIALRSAHELNLAMPGTALTAQLYNAAVAAGYGEDD
                     STSVYRVLEKLANLEQ"
     gene            complement(4228..4428)
                     /locus_tag="VIS73_09725"
     CDS             complement(4228..4428)
                     /locus_tag="VIS73_09725"
                     /inference="COORDINATES: protein motif:HMM:NF025436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative motility protein"
                     /protein_id="HEY5763470.1"
                     /translation="MNVDSATTSAFSAIASQRVGDAVSVSVPSKTLDIQTVGALALID
                     AIPAAPSTSLTVHLGTLINTKA"
     gene            complement(4501..4731)
                     /locus_tag="VIS73_09730"
     CDS             complement(4501..4731)
                     /locus_tag="VIS73_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEY5763471.1"
                     /translation="MSVDRAIRIMAGSFILISLALGVAASPLFVSVHFLWLTAFVGAN
                     LLQSGFTRFCPAETILVKLGVAKADGASCAAR"
     gene            complement(4747..>5199)
                     /locus_tag="VIS73_09735"
     CDS             complement(4747..>5199)
                     /locus_tag="VIS73_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5763472.1"
                     /translation="LVLIYLLVVAQFKSYLVPLIIMAPIPLTIIGVMPGHALLGAQFT
                     ATSMIGMIALAGIIVRNSILLVDFIREQVAAGMPLAEAIVQSAVVRAKPIALTALAAM
                     LGALFILDDPIFNGLAISLIFGIFVSTVLTLVVIPLLYFVAFRRQSAT"
BASE COUNT          854 a         1756 c         1714 g          875 t
ORIGIN      
        1 cacctcctgc gaggttgccg catcgcgcat cagcgcgttc tcgtcgatgt cgagttcgag
       61 ccgcgccggc ggcaggctgg tttcggcgag caggtcgcgc agcttggata ccagcgactt
      121 gtgccagaac tggcgcggcg acaggtcgac taccaccggc acctgcgggt aaccctggcg
      181 cgcccaggcg gccgcctgcg tcgccgcgcg caacaacagg cgctcgccga tcggaatgat
      241 caggccgctt tcctcggcga ccggccggaa gctctcgggc ggcacctcgc cgcgttcggc
      301 atcgtaccag cgcaactttg cctcgattgc gcagacccgg cggcgaccga tgtcgacgac
      361 cggttcgtag acgacgcgca gcccatcggt ttcgacagcg cgccgcagcg cctgctcgag
      421 atcgaacagc cgcgccgtct catcggccat ttcgccggag aagaaccggt agtcgttgcg
      481 cccgccggcc tttgccgcgt acatcgcggt gtcggcggcg cgcagcagtg ccgcgaggtc
      541 ggaaccgtcc tcggggtaga tggcgatgcc gatcgacgcc gacgggtgca gttcgtggct
      601 gccggcggca acggcctcgc gcaggccacc gatgatcttg ccggcgatgc ggcgaacgtc
      661 ctcgactccg ttgacctgct gcgcgagcac gacgaactcg tcgccaccca ggcgcgccac
      721 ggtgtcggac tggcgaaccg ccgcggcgat ccgcgcggcg acgcccttga gaaagatgtc
      781 gccgacctcg tggccgagcg aatcgttaat ctgcttgaaa cggtcgaggt cgatgaagag
      841 cacggcgacc cgctgcccgc tgcggccggc ctgcaccagc gcctgctgcg cgcggtcggt
      901 aaacaggctg cggttgggca agccggtcag cgtgtcgtgg tgggcaatct gccagacccg
      961 ctcctcagcc ttgatgcgat cgaaaacctc ggctttgagc aaggcattgg cttcggccag
     1021 cgccgccgtc cgttcggcaa cgcgctgctc gagatcgcgc tgcgcagcga ggagctgctc
     1081 ctgcatgtcg tggcgatcgg tgacgtcgtc gataatccag atcacgccga tgccgccggc
     1141 ctcctcgaca tcggtgacgg cgtgcccggt cgcccggacc cagaacagac tgccgtcggc
     1201 gcgcttgacc tgccattcgt cggcatagat ctcgtcctgc tcgagcgctg cgtaggcatc
     1261 gcggccaatc acctcgaagt cggcgtgcga ggcgaacagc accgccgtcg agcgaccgtt
     1321 aagtccgccc ggctcgtagc cgaagatcga ttcgagccgc cggttgcagc gctgcacgac
     1381 gcggttcctg accaacagga tgccgacggc ggcgttgtga aagatcgact gctgctcgtc
     1441 gagcgccgcg cgcaggctct cctcgcgggt ccgccgctcg ctgacgtcct cgagcaggaa
     1501 gaccgcgccg gcctgcggtt cgcgcggatc gaagcagcgc ccggtgagcc ggcaccagaa
     1561 cggctggccg tccttgcggc gcaggacctg ctcgcgccgg tgcgtgctgc cgcgcgcgaa
     1621 ggcttcgtcg gtgatcgcgt tgatgccggc gtagtcctcg tcgctggcgt accagagccg
     1681 cgtcgtgtag ccgagcattt cgcccgaggc gtagccaaag atttcctcga agcggcgatt
     1741 gcactgcagc agcacgcggt tggcgattac cgcgacgccg atcggggcgg tctcgaagat
     1801 cgcctcgagc tgtcgcctga cttcgcgttc gcggctgcgc gcctcgcgct cggccgtgca
     1861 gtcctcggcg atccatacag tgccaccggt cggccgtgac ggatcgacgg cgcgccccca
     1921 gatgcgcacc cagaacaggt cgccgtcctt gcgccgcatc tcgacctcgg tctggaagca
     1981 ctcgccctcg gccagtgccg gcgatgcggc agcgccgagc gcctcgtagg cgtcgtcggt
     2041 cggatagagc atcttcgccg ggcagccagt aagttcttcg gccgtatagc cgaacatctc
     2101 gcaccagcgg cggttgcaac ggacgaactg gcggtggtcg gtaaaggcga taccgaccgc
     2161 ggcgttgtcg aatacggcct gctgggcgag cgccggctgg ttgacgcggg cgttcgcccg
     2221 cggtgacgcc tcctgcggtt ccagcttgcg ccccggctgc tgttcgtcca tcggcatcag
     2281 tcgcgtgccg catgtcgcga cagcgcggtc ccggcttcgc cgctcgacgg cggcttgttg
     2341 ttcctaggat tctctcttgg ggaattccag cacgcgcagc aggctgctcg tgtcatcgcg
     2401 gccgaacccc agcgccatca aggcattgag ctgctcggcg acggcggcag tcagtggcat
     2461 cgccaccccc atcgcccgcg cttcagaggc gattattgca tagtctttat gatgcagacg
     2521 ggcctggacg ccggccgcga agtcgccgcg gaccattcgc ccgccaaaga cttcgagcgc
     2581 gcgcgaggcc gccgacccgc ccgacagcgc cttcagcccg gcctcgccgt caacgccggt
     2641 ggcatgccaa agcgagaccg cctcggcgca ggcctcgatt gtcgcgacca tgatcatctg
     2701 gttgcagatc ttggcgacct gacccgcacc aaccggtccg atatggacga tggtctttcc
     2761 gagcgcctcg aagatcggca tcacccgctc caccggccca tcgtcgccgc cggccatgat
     2821 cgacaggctt gccgctgccg ccccggtcgg accacccgac accggcgcgt cgatgaagtg
     2881 cacgccgcgc tcggcatagc tcgctgcgag atcccgcgcc gtcgatggcg cgatcgtgct
     2941 catgtcgagg tgaatcgagt ccctggcaaa tccctcgcgc aggccctgtg ggcccagcgc
     3001 gatctcgcgc acatcctcgt ccttggtcac catcgtgatc acgacgtcgc tgcgccccgc
     3061 cagcgcagcc gccgaattga accagccagc gccaccgccg accagttctt cggccgcctc
     3121 acgtcgccgg gcccagacgc ccaattcgtg gcccgcggcg agtagatggc cggccatgtg
     3181 ccggcccatg tcgccgagac cgatgaatcc gatcttcatc ggccgacctc tattgctcaa
     3241 ggttcgcgag tttctccagg acgcgataga ccgaggtcga atcgtcctca ccgtagccgg
     3301 ccgccaccgc ggcgttatag agctgcgcgg tcagcgccgt gccgggcatc gccagattca
     3361 gctcatgggc gctgcgcaac gcgatatgca ggtccttctg gtgcatccag gccttgaagc
     3421 cgggcttgaa gttgcggtcg agcaggcgct ggccgtgcag gtcgagcact ttgctgttgg
     3481 caaagccgcc caggagcgcc tcgcgcacct tggccggatc gacgcccgat ttccgcgcca
     3541 gacaaaaggc ttccgagacg gcgatgacgg tcatcgccac caccaggttg ttggcggcct
     3601 tggtcacctg tccggcaccc gtctcgccga tgtagacgat gttcttgccc atgcattcga
     3661 agaccggctt ggccttgtcg aaagcggcct tgggcccgcc gaccatgatc gacagcgtgc
     3721 cgttgattgc gccgacgtcg ccgcccgaaa ccggcgcgtc gaggaattcg attcccttct
     3781 tgccgagttc ggcgttgacc tgcttcgccg tcgacggctg gatcgtgctc atgtcgatgg
     3841 caatcaggcc cttgttgccg ccctcgatca ccccgtcctt gccaagcatc acctgctcga
     3901 cgtcagggcc gtcggcgacc atcgagacga caacctcaac cccggcggcc agcgccttcg
     3961 ggctggccat gaccttggcg ccggcatcgg cgagcggctt ggtcgattcg acgcgccgcg
     4021 cccagaccga cacggcatgc ccgcccttga tcaggttgag ggccatgggg cggcccatca
     4081 ggccgagtcc gatgaatccg atacgcatag tggtctcctg ttcttgttgg ctttgccggc
     4141 acggagcgac cgctggggca agcgaggttt tgactcggaa tcgcgcagcc tgtttcggcg
     4201 ctagccgcgc aagtcgtaag acgcggctca ggctttcgta ttgatcagcg taccaaggtg
     4261 caccgttagg ctcgtgcttg gcgcggccgg gatcgcgtcg atcagcgcca gcgcgccgac
     4321 cgtctgaatg tcgagcgtct tgctcggcac gctcacgctg acggcatcgc cgacgcgttg
     4381 ggaagcgatg gcgctgaagg cactggtcgt ggcgctgtca acgttcatgg gcggctcgat
     4441 gcagaggtga ctaccggaac aagatagccc cggcagcctt ggcgatcaag cggccgtcga
     4501 tcagcgcgcg gcgcagctgg cgccatcagc cttcgcgaca cctagcttga cgaggatcgt
     4561 ttctgccgga caaaagcgcg tgaagccact ctgcagcagg ttggcgccga caaaggcggt
     4621 gagccagagg aagtgcaccg agacaaacag cgggctcgcc gcgacgccga gggcgagcga
     4681 gataagaatg aaactgccgg ccatgatgcg gatggcgcga tcgacggaca tgaagatctc
     4741 cttggttcag gttgcggatt gacgtcggaa ggcgacgaag taaagcagcg gaatcaccac
     4801 cagcgtcagc acggtggaga cgaagatgcc gaagattagg ctgatcgcca gcccgttgaa
     4861 gatcggatcg tcgaggatga acagcgcgcc gagcatggcg gcgagcgcgg tcagcgcgat
     4921 cggcttggca cggaccacgg ccgactgcac gattgcctcg gccagcggca tgccggcggc
     4981 gacctgctcg cggatgaagt cgacgagcag gatcgagttg cggacgatga tgccggcgag
     5041 cgcgatcatg ccgatcatcg aggtggcggt gaactgcgcg ccgagcagcg cgtgtccggg
     5101 catgacgccg atgatcgtga gcgggatcgg cgccatgatg atcagcggca ccagatagct
     5161 cttgaactgc gcgaccacca gcaggtagat cagcaccag
//
LOCUS       DATDAA010000317         7459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_408254,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000317 DATDAA010000000
VERSION     DATDAA010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408254"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /locus_tag="VIS73_09740"
     CDS             <1..1580
                     /locus_tag="VIS73_09740"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEY5763473.1"
                     /translation="EKAAAEGQPAPEMKMPKSKFDEAKAYQTKLAYVFGGIATVVGLL
                     ILGVKPKEGNFDYFLSIFPGMAYILLIAFVVRWGLDPMFGNWGKAAEPTLGWNFARIF
                     NLNYVVLGIVIGIIIVNVFKIPDWAANGVRTARFFLKTGVILLGALYSATELAQLGAL
                     SVVMIGVFVLGSVWMVLVMGKRMQAGNSMTGVLSAGMGVCGVSATVAASPVVNAKAGE
                     IAYTIGTILVWGVGCMFIFPTIGHLLGMGPVQFGAWAGTGILNSAQVAGAALAFDPKG
                     IETLKVAEIFNITRVLFLPIIVLWLAAWYVKHEEGAQQVNLTQVLVSKFPLFVLGFIL
                     MFALSSMGAFTPAGHYQGKYFSADKVKEESLLKEKDVVALQSELDRLRAPEGSAQLDQ
                     HFADKAVTVGKHKATAADAHQAMADLIANRKNVSRQNDDVLKAVAKLSGLDEGAKGAL
                     DKAHKAVWHTSKTIAAFRDWLAWLFAFGLIGLGMQITTAALKQAGGKPLVIGGVVGTV
                     KAIGSLIVVLLFVREFI"
     gene            1593..1721
                     /locus_tag="VIS73_09745"
     CDS             1593..1721
                     /locus_tag="VIS73_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763474.1"
                     /translation="MSEQKFERGTNLSIFVSDRREDIGALIFALVIAIGIVAFVGG"
     gene            1729..2253
                     /locus_tag="VIS73_09750"
     CDS             1729..2253
                     /locus_tag="VIS73_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5763475.1"
                     /translation="MRESRAPELMPGVPAVPMRVMKAPASLLLCHHGTAGARAAAGLA
                     LELAEPGTTTLIHCLVVPDLWAGMQGDDWLNNASTRDDFGRYVEQMLEDDARREFADV
                     EARCRESNIACESIMRLGEPTETVLRVAAETAADLVVIGPPRRKGEEGLRSRIDLDRL
                     LRGLHCPLLIAPRP"
     gene            2223..2840
                     /locus_tag="VIS73_09755"
     CDS             2223..2840
                     /locus_tag="VIS73_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763476.1"
                     /translation="MPPADCAAPVAPDGDSAHAETVLPLAQAEVEHFVRDPERLWRLN
                     PHIHIESWQALPDGFRCHLRNELNGFEGELAVRVDEVVSGAERQLRYDYARGLRLCTE
                     ITVATDGDGSRLTLVDRYPRVEDPEDPRLVEVDRSLVPWLAAIRRHLLARQRWGWLPG
                     WRWWNEHLLAGMPPRSRRIVRLIVWISAIEFVLFAAVVVVWRLMD"
     gene            complement(2862..3248)
                     /locus_tag="VIS73_09760"
     CDS             complement(2862..3248)
                     /locus_tag="VIS73_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763477.1"
                     /translation="MKIRFIAIAAVLVTSLGALAPTALAADPQPHSVAALNENRATLA
                     GKTIQVAGKVVKVNNGIMGRNFVHVQDGSGDAATGSNNLIVTSKQTARVGDQVAVSGI
                     VVVDRDFGSGYLYPLLIEEASITPKP"
     gene            complement(3350..4633)
                     /locus_tag="VIS73_09765"
     CDS             complement(3350..4633)
                     /locus_tag="VIS73_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763478.1"
                     /translation="MLTLRRLGDALSLQEREGPPVALAALYGFCILFAYYLLRPVRDE
                     IAAADRGNLQVLWTAVFLVMLLAVPLYSTAVARLRRGLFIPLANRFFAVNLLLFYAAL
                     VLLPESARPWIDRTFYVWLSVFALFVVTIFWGLVVDLFRDEQGRRLFGLIAVGASLGG
                     IAGSATTAALAGHLPVFVLLLIAVLPLEVANWLARALDRRAEHAPTQLQREPEAPIGG
                     SAFSGIALVFHDPQLRRIAAWILLMTFASTMLYFLQANLLGEAVTDRGERREFLARID
                     LAVNLLTLTGQALLAAPLIARLGLGVTLALLPMVAATGFAVLGSALLALPTGPTLAAL
                     AVVRVLYDATRHALAKPAREVLFTRVPREARYKSKAFIDAAVYRGGDLLSGWCYVGLV
                     AIGLGGGLIALLAVPVAIVWALLGRRLAGALATGS"
     gene            complement(4650..5588)
                     /locus_tag="VIS73_09770"
     CDS             complement(4650..5588)
                     /locus_tag="VIS73_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5763479.1"
                     /translation="MRRFASISRRRWLAAGAALGATALLPARTSHAGTLGVMATRAIP
                     GTGERLPVIGLGTNRYYAGDLVNLRRLRDTLLSFARNGGKVIDTAPAYGESQRIIGDI
                     LGEAGLVSRLFLATKVDRENPVAAEASIARSLKLLNTPRIDLMQLHNLRGVDTTLPLL
                     REQQAAGRIRYIGITSARDKQYEEMEAVIRREPLDFIQIDYAVGNRSAADRLLPLAAD
                     RGMATLISLPFGGGRQFAAVEGHPLPPLAAEIGCRSWAQLFLKYVLSHPAVTCVIPGT
                     TQPAHVEENLHAGHGPLPDAAMRRAIERHYDAIVGA"
     gene            complement(5599..6312)
                     /locus_tag="VIS73_09775"
     CDS             complement(5599..6312)
                     /locus_tag="VIS73_09775"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY5763480.1"
                     /translation="MPHLLRQLRPLAIAAGVLLSSFAIAAEAPLRIGIIGTGKIGGAL
                     AEHWAKAGHELVISSRHPEQLKDLAASLGPKVRAGTPAEAAAFGEVILISVPYGALPQ
                     VGRDYATAMRAKVVLETGNPFPGRDGAMAEPARAKGTGAASSEYLPGVRLVRAFTSVP
                     HWSIRDDAHRSGERVAIPLAADDGTALKLAERLVRDAGFEPVVVGGLAEARRFDVGSA
                     AFGRAMTARELRATLRLEQ"
     gene            complement(6321..7250)
                     /locus_tag="VIS73_09780"
     CDS             complement(6321..7250)
                     /locus_tag="VIS73_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5763481.1"
                     /translation="MSRARFLRLAAGAAAAAVATPWLAQAAERPDGSPSRQPMARRRV
                     PSSGEMLPVVGLGTARVFDHAPGSAEYQRLPAVLAALFAAGGSVIDSSPMYGRAERTI
                     GELLAAASSRDQAFLATKVWTRGRDEGRRQMEESRALLQASKIELMQIHNLVDWRTQL
                     ATLREWRAEGRIRYLGITHYTASAYDELEAVMRAERFDFLQVNYSLDEDEAARRILPL
                     AAERGIAVLVNRPFGGGGLLRRLRDRRLPPWAAEAGATSWAQLLLKFALAHPAVTCVI
                     PGTSRAEHMVDNAAAGNGPLPDDAFWRRHRDSI"
BASE COUNT         1232 a         2376 c         2608 g         1243 t
ORIGIN      
        1 ccgaaaaggc cgcggcggaa ggccagccgg cgcccgagat gaagatgccg aagtcgaaat
       61 ttgacgaggc caaggcctat caaacgaagc tggcctacgt cttcggcggc atcgccaccg
      121 tcgtcggcct gctgatcctc ggcgtcaagc cgaaggaagg caacttcgac tatttcctgt
      181 cgatcttccc cgggatggcc tacatcctgc tgatcgcttt cgtcgtgcgt tggggactag
      241 acccgatgtt cggcaactgg ggaaaggcgg cggagccgac gctgggctgg aacttcgcgc
      301 gcatcttcaa cctgaactac gtagtgctcg gcatcgtcat cggcatcatc atcgtcaacg
      361 tcttcaagat ccccgactgg gcggcgaacg gcgtgcgcac cgcgcgcttc ttcctgaaga
      421 ccggcgtgat cctcttgggc gcgctctaca gcgcgaccga actggcgcag ctcggcgcgc
      481 tgtcggtggt gatgatcggc gtcttcgtcc tgggttcggt atggatggtg ctggtcatgg
      541 gcaagcgcat gcaggccggc aattcgatga ccggcgtgct ctcggccggc atgggcgtct
      601 gcggcgtctc ggccaccgtc gccgcatcgc cggtggtcaa cgccaaggcc ggcgagatcg
      661 cctacacgat cggcacgatt ctggtctggg gcgtcggctg catgtttatc ttcccgacca
      721 tcggccacct gctgggcatg gggccggtgc agttcggcgc ctgggccggt accggcatcc
      781 tcaattcggc gcaggtcgcc ggcgcggcgc tggccttcga cccgaagggc atcgagacgc
      841 tgaaggtcgc cgagattttc aacatcacgc gcgtgctatt cctgccgatc atcgtgctct
      901 ggctcgccgc ctggtacgtc aagcacgagg aaggcgcgca gcaggtcaac ctgacccagg
      961 tgctggtctc gaaattcccg ctgttcgtcc tcggcttcat cctgatgttc gcgttgtcgt
     1021 ccatgggcgc cttcacgccg gccggccact accagggcaa gtacttcagc gccgacaagg
     1081 tgaaggagga gtcgctgctc aaggagaagg acgtcgtggc gctgcagtcc gagctcgatc
     1141 gcctgcgcgc gccggaaggt tccgcccagc tcgaccagca cttcgccgac aaggctgtca
     1201 ccgtcggcaa gcacaaggcg accgccgctg atgcgcatca ggcgatggcc gacctcatcg
     1261 ctaaccggaa gaacgtcagc cgccagaacg acgacgtact gaaggcggtg gcgaaactgt
     1321 ccgggctgga tgagggcgcc aagggcgcgc tggacaaggc gcacaaggcc gtctggcata
     1381 cgtcgaagac gatcgccgcc ttccgcgact ggctggcctg gctgttcgct ttcggcctga
     1441 tcgggctggg catgcagatc accaccgcgg cgctcaagca ggccggcggc aagccgctgg
     1501 tcatcggcgg cgtggtcggc accgtcaagg cgatcggttc gctgatcgtg gtcctgctgt
     1561 tcgtgcggga attcatttaa aggagaacgg acatgagcga acagaaattc gagcgcggca
     1621 ccaatctgtc gatcttcgtc agcgaccgcc gggaggacat cggcgcgctg atctttgccc
     1681 tggtgatcgc catcggcatc gtggctttcg ttggcggctg aggccggcat gcgcgaatcg
     1741 agagccccgg agctgatgcc gggtgtgcct gcagtgccga tgcgggtgat gaaagcgccc
     1801 gccagcctgt tgctctgcca ccacggaact gccggggcac gggccgccgc tgggctggcg
     1861 ctggagcttg ccgagcccgg tacgactacg ctgattcact gcctggtcgt tcccgatctg
     1921 tgggctggca tgcaggggga tgactggctc aacaatgcct cgacgcgaga cgattttggc
     1981 cgctacgttg agcagatgct ggaggacgac gcgcggcgtg agttcgccga tgtcgaggcg
     2041 cgctgtcgcg aatcgaatat cgcctgtgag tcgatcatgc ggctgggcga gcccaccgag
     2101 actgtgctgc gggtggccgc cgagacggcc gccgatctcg tcgtgatcgg cccgccgcgg
     2161 cgcaagggtg aggagggact gcgttcgaga atcgatctcg accggctgct gcggggtttg
     2221 cattgccccc tgctgattgc gccgcgcccg tagcgccgga cggcgacagc gcccacgccg
     2281 aaaccgtcct gccgctggcg caggccgagg tcgagcactt cgtccgtgat cccgaacggc
     2341 tctggcggct caatccgcat attcatatcg aaagctggca ggctctgccc gatggttttc
     2401 gttgccactt gcgcaatgag ctgaacggct tcgagggcga attggccgtg cgcgtcgacg
     2461 aagtcgtttc cggcgcggaa cggcagcttc gttacgacta cgcgcgtggc ctgcgcctct
     2521 gcaccgaaat caccgtcgcc actgatggcg acggttcgcg gctgacgctg gtcgatcgtt
     2581 atccacgcgt agaggatccg gaagacccgc gcctagtcga ggtcgatcgc agtctggtgc
     2641 cttggctcgc ggcaatccgt cgccacctgc tcgcccggca acgctggggc tggttgccgg
     2701 gttggcgctg gtggaacgag catctcctgg ccgggatgcc gccgcgcagc cggcgcatcg
     2761 tgcgcctgat cgtctggatc agcgccatcg agttcgtgct tttcgcagcg gttgtcgtcg
     2821 tctggcggct tatggactga gccgcagcga tcggccggcg ctcaaggctt gggcgtgatg
     2881 ctcgcttctt cgatcaacag cggatagaga tagccgctgc cgaaatcgcg gtcgacgacg
     2941 acaatgccgg aaaccgcgac ctggtcgccg actcgtgctg tttgcttgct ggtgacgatc
     3001 aggttgttcg agccggtggc ggcgtcgccg ctgccgtcct gcacgtgaac gaagttgcgg
     3061 cccatgatgc cgttgttgac cttgaccacc ttcccggcga cctggatcgt cttgccggcg
     3121 agcgtggcgc ggttctcgtt aagtgcggcg acactgtgcg gctgcggatc ggcggctagc
     3181 gctgtgggag cgagcgcgcc gagggacgtg acgagaacgg cggcgatggc gatgaatcgg
     3241 atcttcatgg gaattccttt gcagagggga tgagcggcac tagtcggtgg ccgcgattat
     3301 agcgacggca tcggctttcc cggcgcacga gcttgccgct tgtcgatgac tacgaaccgg
     3361 ttgccagcgc gccggcgagt cggcggccga gcagggccca gacgattgcc accggtaccg
     3421 cgagcagcgc gatgaggccg ccgccaaggc cgatcgccac cagcccgacg taacaccatc
     3481 cgctcagcag gtcgccgccg cgatagaccg cggcgtcgat gaaggccttc gacttgtagc
     3541 gcgcctcgcg cggcacgcgg gtgaacagca cttcgcgcgc cggcttggcc agcgcgtgcc
     3601 gggtcgcgtc gtagaggaca cggacgacgg ccagcgccgc tagcgttggg ccggtcggca
     3661 aggccagcaa tgccgagccg agcacggcga agccagtcgc cgcgaccatc ggcagcaggg
     3721 cgagggtcac gccgaggccg agccgggcga tcagtggcgc agcgagcagc gcttggccgg
     3781 tgagcgttag cagattgact gcgaggtcga tgcgcgcgag gaattcgcgg cgttcgccgc
     3841 ggtcggtgac cgcttcgccg agcaggttgg cctgcaggaa gtagagcatg gtcgaagcga
     3901 aggtcatcag cagaatccac gccgcgatgc ggcgtagctg cgggtcatga aagaccagcg
     3961 cgatgccgct gaaggcgctg cccccgatcg gcgcctcggg ctcgcgctgc agttgcgtcg
     4021 gtgcgtgctc ggcgcgccgg tcgagcgcgc gagcgagcca gttggccact tcgagcggca
     4081 gcactgcaat cagcaggagc acgaataccg gcaggtgtcc agccagcgcc gccgtcgttg
     4141 ccgatccggc gatgccgccg agcgaggcgc cgacggcaat gagaccgaac agccggcggc
     4201 cctgttcgtc gcggaacagg tcgacgacca agccccagaa gatggtgacc acgaatagcg
     4261 cgaagacgct cagccagaca tagaaggtcc ggtcgatcca cggccgggcc gattcgggca
     4321 gcaataccag cgccgcgtag aacagcagca ggttgactgc gaagaagcgg ttggccagcg
     4381 ggatgaagag gcctcggcgg aggcgggcga ccgccgtcga gtagagcggc acggcaagca
     4441 gcatgacgag gaagacggcg gtccacagaa cctgcaggtt gccgcggtcg gcggcggcga
     4501 tttcgtcgcg caccgggcgc agcaggtagt aggcgaagag gatgcagaag ccgtagagcg
     4561 ccgccaacgc gaccggcggt ccctcgcgct cttgcagtga cagcgcgtca ccgagtcggc
     4621 gcagcgtaag catgcggccc gccgccacct caggcgccaa cgatggcgtc gtaatggcgt
     4681 tcgatcgcgc gccgcatcgc cgcatcgggt agcggcccgt gacctgcgtg cagattttcc
     4741 tcgacgtgcg ctggctgcgt cgtgccggga atcacgcagg tgacggccgg gtgcgacagg
     4801 acgtacttga ggaacagttg cgcccagctg cggcagccaa tctcggctgc caacggtggc
     4861 agcggatgtc cttcgactgc cgcgaactga cggccaccac caaaggggag gctgatcagc
     4921 gtcgccatgc cgcggtcggc ggccaagggc agcaggcggt ccgccgcgct gcgattgccg
     4981 acggcgtagt cgatctggat gaagtcgagc ggttcgcggc gaataaccgc ttccatttcc
     5041 tcgtactgct tgtcgcgcgc gctggtgatt ccgatatagc ggatgcggcc cgccgcttgc
     5101 tgctcgcgca acagcggcag cgttgtatcg acgccgcgca ggttgtgcag ctgcatgagg
     5161 tctatccgcg gcgtattaag cagtttgagc gaacgcgcga tgctcgcttc ggcggcgacc
     5221 gggttttcgc ggtcgacctt ggtcgccagg aagaggcggc tgaccagacc cgcttcgccg
     5281 aggatgtcgc cgatgatgcg ctgggattcg ccataggctg gcgcggtatc gatgaccttg
     5341 ccgccgttgc gggcgaagct cagcaacgtg tcgcgcaggc ggcgcaggtt gacgaggtcg
     5401 ccggcgtagt agcggttggt gccgagcccg atgaccggta ggcgctcacc ggtgcctgga
     5461 attgcccgcg tcgccatcac gccaagcgtg cccgcatggc tggtcctcgc cggcagcagg
     5521 gcggtcgcgc ccagtgcggc gccggcggcc agccagcggc gccgggaaat cgaggcaaac
     5581 cttcgcatca ccatactttc attgctccaa cctgagtgtg gcgcgtaact cgcgcgccgt
     5641 catcgcccgg ccgaacgccg ccgaaccgac atcgaagcgg cgggcctcgg cgagtccgcc
     5701 gaccaccacc ggctcgaagc cggcgtcgcg gaccaggcgt tcggcaagct tcaatgcggt
     5761 cccgtcgtcg gctgcgaggg ggatcgcgac gcgctcgccg ctgcgatgcg catcatcgcg
     5821 aatcgaccag tgcggcaccg aggtgaaggc gcgcaccagc cgtacgccgg gcaggtactc
     5881 cgacgaggcg gcgccggttc cctttgcgcg ggccggctcg gccatcgcgc cgtcgcggcc
     5941 cggaaatgga ttgccggtct cgagcacgac cttggcgcgc attgcggtcg cgtaatcgcg
     6001 gccgacctgc ggcaacgcgc catagggtac ggaaatcagg atcacctcgc cgaaggcggc
     6061 ggcttcggcc ggcgtgccgg cacggacttt aggaccgagg cttgcggcga gatccttcag
     6121 ctgttcgggg tgccgcgagg aaatcaccag ttcatggccg gccttggccc agtgttcggc
     6181 gagcgcgccg ccgatcttgc cggtgccgat gatgccgatt ctcagcggtg cttcggcggc
     6241 gatagcgaac gacgaaagca gtacgccggc ggcgatggcg agagggcgaa gctggcgaag
     6301 gaggtggggc atcgggcttc tcagatcgaa tcccggtggc gacgccagaa ggcatcgtcg
     6361 ggcaaggggc cattgcccgc cgcggcattg tcgaccatgt gctcggcccg gctggttccg
     6421 ggaatgacgc aggtaacggc ggggtgggcg agcgcgaact tgagcagcag ctgcgcccag
     6481 ctcgtcgcgc cagcttcggc tgcccacggg ggcagccggc ggtcgcgcag gcgacgcagc
     6541 aaaccgccgc cgccaaacgg ccgatttacc agcaccgcga tgccgcgctc ggcggccagc
     6601 ggcaggatgc gtcgcgccgc ctcgtcctcg tcgagcgaat agtttacctg caggaaatcg
     6661 aaacgctcgg cgcgcatcac cgcctcgagt tcgtcgtagg cgctcgccgt gtaatgggtg
     6721 atgccgagat aacggatgcg gccttcggcg cgccattcac gcagggttgc cagctgggtc
     6781 cgccagtcga cgaggttgtg aatctgcatc agctcgatct tgcttgcctg caacagcgct
     6841 cgcgattcct ccatctgccg tcgtccctcg tcgcggccgc gtgtccatac ctttgtcgcg
     6901 agaaaggcct ggtcgcggct actggcagcc gccagcaatt cgccgatggt ccgctcggca
     6961 cggccgtaca tcggcgagct atcgatcacc gagccgccgg cggcgaacag agcggcaagc
     7021 acagcgggca gacgctggta ctcggcgctg cccggcgcgt ggtcgaaaac gcgcgcagtg
     7081 ccaagaccga ccaccggcag catttcgccg ctgcttggca cgcgtcgccg ggccatcggc
     7141 tggcggctag gcgaaccgtc tggcctctcg gctgcctgcg ccagccaggg tgtcgcgact
     7201 gcggccgccg cggcgccagc ggccagccgc agaaaccggg cgcggctcat cgcgggcgag
     7261 gagtctgggg tagctttcgt tcggttttcg ggtggggaca tcgcgaactc cgataccaat
     7321 gtagcccgct tggcgccggc atcgcggttc tgaccagcag cgcgcacatg aagtgcccga
     7381 gacgatgcga attggtcacg atcgtgaatg cccctgcagt gtagagtcgg ctcgccgggg
     7441 cctattcccc aggctgaca
//
LOCUS       DATDAA010000318         2483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_303952,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000318 DATDAA010000000
VERSION     DATDAA010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303952"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VIS73_09785"
     CDS             complement(<1..943)
                     /locus_tag="VIS73_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5763482.1"
                     /translation="MTPPPAADLLDQLRGRIGAGNVLTAAGDVAPYLTDWRGRYTGSA
                     RAVLRPGSTAEVAAVVACCAAARVAIVAQGGNTGLVGGATPDADGHAVVLSLARLNAV
                     RTIDLANNTLTAEAGCTLSAVREAADREGRLFPLSLAAEGSCTIGGNLATNAGGVQVL
                     RYGNARALCLGLEVVLPDGQVWGGLRGLRKDNAGLDLKQLFIGSEGTLGIITAAVLKL
                     VTKPAACATAWLAVPDVAAAVAVMGRLQTRFPERLDSYELISRATLDLTLAELPGTSD
                     PLPGARTPWLVLLELTDGGEAETLRNALESTLAGDIEA"
     gene            complement(940..1881)
                     /gene="rssA"
                     /locus_tag="VIS73_09790"
     CDS             complement(940..1881)
                     /gene="rssA"
                     /locus_tag="VIS73_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HEY5763483.1"
                     /translation="MQRPRIALALGSGSARGWAHIGVLRELQAAGIEADVVAGTSIGA
                     LVGAAYAANELDALEGWVTKMGWRDVLALVDPVFAGGLLKGDRLMTRFGAHFDVTDFS
                     GMSRPFGCVATDLSTGHEIWLREGKVLDAVRASIAMPGLFSPVKHNGGFLVDGALVNP
                     VPVSLARAMGGQIVIAVELGADLVGYRRRQRQASAEQVNDERGGEGEGDASSPSGMRW
                     FDSLRKLLPARIGNGDGAESGSLPVPTILDVMNTSINIMQVRIARSRLAGEPADVLVQ
                     PRLATIGLLDFHRAEESIAEGRAAMRLVLPAVHRLLE"
     gene            complement(1885..>2483)
                     /locus_tag="VIS73_09795"
     CDS             complement(1885..>2483)
                     /locus_tag="VIS73_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5763484.1"
                     /translation="LMAEGKILPYLDVPLQHASQRILKAMKRPASAENALARIRAWRA
                     VCPDITIRSTFIAGFPGETEADFEELLSFLEEAELDRVGCFAYSDVDGATANALPGAL
                     PEEVREERKSRLMWVQEDISARRLAARIGQRMAVLVDEIEEDGIVVARSPGDAPDIDG
                     LVFIDGLQDYEVNPGDFIEVEIVDADTHDLFAQPVVEA"
BASE COUNT          450 a          860 c          786 g          387 t
ORIGIN      
        1 cggcttcgat atcgccagcc agagtcgatt caagcgcatt gcgcagggtt tccgcttcgc
       61 caccatcggt cagctccagc aatacgagcc aaggtgtcct cgcacccggc aaggggtcac
      121 tggtgcccgg aagctcggcc agcgttaaat ccagcgtcgc ccgcgagatc agttcgtagc
      181 tgtccagccg ttccggaaag cgggtctgca gcctgcccat gaccgcgacc gccgctgcca
      241 cgtcgggcac cgccagccat gcggtggcac acgctgcggg cttcgtcacg agcttgagga
      301 ctgcggcggt gatgatgccg agggtaccct ccgagccgat gaagagctgc ttgagatcga
      361 gcccggcgtt gtccttgcgc aggccgcgca atccacccca gacctggcca tcgggcagca
      421 ccacttcgag tccaaggcac agcgcgcggg cattgccgta gcgcagcacc tgaacgccgc
      481 cggcgttggt ggcgaggttg ccgcctatcg tgcaactgcc ctctgcggcc agcgaaagcg
      541 ggaacaggcg cccctcgcgg tcggccgcct cacgcaccgc tgagagcgta cagccagcct
      601 cggcagtcag cgtgttgttg gcgaggtcga tcgtgcgaac ggcattcagc cgagccagcg
      661 aaagcacgac cgcgtgtccg tcggcatcgg gcgtcgcgcc gccgaccaaa cccgtattgc
      721 cgccctgggc gacgatggca acgcgcgctg ccgcacagca ggcaacgacg gcggcgactt
      781 cggcggttga gcccggccgc agcaccgcgc gagcacttcc cgtgtagcgg ccacgccagt
      841 cggtgagata cggtgcaaca tcgccggcag cggtcagcac gttgccggca ccaatccggc
      901 cacgcagctg gtcgagcaga tcggctgccg gcggtggcgt cattcgagca gtcggtgcac
      961 ggccggcagc actaggcgca tcgcggcacg accctcggcg atcgactcct cggcgcgatg
     1021 gaaatcgagc agcccgatcg tggccagtcg cggctggacc agcacgtcgg ccggttcgcc
     1081 ggcgagccgg ctgcgggcaa ttcgaacctg catgatgtta atgctggtgt tcatgacatc
     1141 gaggatggtc ggcaccggca ggctgcccga ttcagcccca tcgccgtttc caatccgggc
     1201 cggcagcaac ttccggagac tgtcgaacca gcgcattccg ctcggcgaac tcgcgtcccc
     1261 ctcgccctcg ccgccacgtt catcgttgac ctgttctgca cttgcctgac gttgacgccg
     1321 acgatacccg acgaggtcgg cgccgagttc gacggcgatg acgatctgcc cgcccatcgc
     1381 gcgcgccagc gacacgggaa ccggattaac cagcgcaccg tcgaccaaaa agccaccgtt
     1441 gtgcttgacc ggtgaaaaga ggcccggcat cgcgatcgaa gcacgaaccg catcgagaac
     1501 cttgccttcg cgcagccaga tctcgtgccc agtcgacaga tcggtcgcga cgcacccgaa
     1561 gggcctgctc atgcctgaaa aatcggtcac gtcgaaatgc gcgccaaaac gcgtcatcag
     1621 tcgatcgccc ttgagcagcc caccagcaaa gaccgggtcg accagcgcga gtacatcgcg
     1681 ccagcccatc ttcgtcaccc agccttcaag ggcgtcgagt tcgttggcgg cgtaagccgc
     1741 gccgaccagg gcgccaatcg acgtgccggc aacgacatcg gcctcgatgc cggcggcctg
     1801 cagctcacgg aggacaccga tgtgcgccca accgcgcgcc gagccgctac cgagtgcgag
     1861 tgcgatgcgt ggacgctgca tcgctcatgc ctccaccacg ggttgcgcaa aaagatcgtg
     1921 cgtatcggcg tcgacgatct cgacttcgat gaagtcgccg ggattgacct cgtagtcctg
     1981 caggccgtcg atgaagacca ggccatcgat gtctggcgcg tcgccgggcg aacgcgcaac
     2041 gacgataccg tcctcctcga tctcgtcgac cagcacggcc atgcgctgcc cgatccgggc
     2101 cgcaaggcgg cgcgccgaaa tgtcttcctg aacccacatt agccgcgact tgcgctcctc
     2161 gcggacttcc tccggcaatg cgccgggcag tgcgttggcc gttgcgccat cgacatcgga
     2221 gtaggcaaaa cagccgacgc gatccagctc ggcctcctcg aggaaagaaa gcaactcctc
     2281 gaagtcggcc tcggtctcgc cgggaaagcc ggcgatgaag gtgctgcgga tcgtgatgtc
     2341 gggacagacc gcccgccacg cgcgaatccg cgccagcgcg ttctcggccg atgctggccg
     2401 cttcatggcc ttgagaatcc gctggctggc gtgctgcagc ggtacgtcga ggtagggcag
     2461 gatcttgcct tcggccatca acg
//
LOCUS       DATDAA010000319         3287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_93760,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000319 DATDAA010000000
VERSION     DATDAA010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93760"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VIS73_09800"
     CDS             complement(<1..915)
                     /locus_tag="VIS73_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEY5763485.1"
                     /translation="MSASEPLWRRAISGAQILFVAFGALVLVPILTGLDPSLALLGAG
                     VGTLIFQALTGRQVPIFLGSSFAFIAPIIYSVQTWGVPATMGGLACASLMYFVLAALV
                     RRHGADFIHRMMPPVVIGPIIMIIGLSLAAVAVNLAMGKTGDGKAELMSYGTSLVLAG
                     ISLAVTIGVSVWAKGFFRLVPILAGVGAGYLAAVLLGLVDFSQVAEAPWIAMPQFVTP
                     EWNIAAILFMIPVALAPAIEHVGDVIAIGGVTGKDYTEKPGLHRTLAGDGAAVAVAGF
                     IGGPPVTTYSEVTGAVMLTKNYNPAIMTW"
     gene            complement(997..1623)
                     /gene="upp"
                     /locus_tag="VIS73_09805"
     CDS             complement(997..1623)
                     /gene="upp"
                     /locus_tag="VIS73_09805"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY5763486.1"
                     /translation="MPVVAVHHPLVQHKIGLMREAEISTKKFRELTAELARLLTYEAA
                     RDFDLEACTIAGWAGPVEVQQIKGKKVTVVPILRAGLGMLDGVLDLIPGAKVSVVGIA
                     RNEQTLQPVPYFERFVGKLDERLALIIDPMLATGGSLIATIDMLKRKGARQIKALVLV
                     AAPEGIRALEAAHPDVHVYTAAIDSHLNEHGYIIPGLGDAGDKIFGTK"
     gene            complement(1702..2493)
                     /locus_tag="VIS73_09810"
     CDS             complement(1702..2493)
                     /locus_tag="VIS73_09810"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY5763487.1"
                     /translation="MLLELVLLFLAGVFGGVLNSVAGGGSFITFPALMWVGVAPISAN
                     ATNTFASCAGYLSGVYALRRDLVAHRAELPRLVAISLLGGIAGAWLLLQTPEAVFRAA
                     IPWLLLFATVLFIYGGQINQQLKALAAGHRHASAVGAILLAVLLLAVCVYGGFFNAGL
                     GIITLSYLALAGHSNINAMNGLKLLVSTAVSLIAIVLFIANGAIAWYEGTIVLAGTLL
                     GGYVAAHVSRKLPQQQVRRFVIVASVAITAYFFYATYFVAPAAVG"
     gene            complement(2675..3085)
                     /locus_tag="VIS73_09815"
     CDS             complement(2675..3085)
                     /locus_tag="VIS73_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763488.1"
                     /translation="MIRQALLAASLLAASTGALATDFGHVERHVLRPVEHLTLSVGSL
                     LHDGFNITYGIGGHRHENRSHSSRVIVVPPPVRVIHRHRHESRHSHWNRHDHGRRAWR
                     DHDRHDDRRGWGNKRRGDDRWDDRRGRDRNRSRH"
BASE COUNT          593 a          986 c         1170 g          538 t
ORIGIN      
        1 ccaggtcatg attgccgggt tgtagttctt ggtcagcatg acggcgccgg ttacttcgga
       61 ataggtggtc accggcggcc cgccgatgaa gccggcgacc gcaaccgcgg cgccgtcgcc
      121 ggccagcgtt cggtgcaggc cgggcttctc ggtgtagtcc ttgccggtga cgccgccgat
      181 ggcgatgacg tcgccgacgt gctcgatcgc cggcgccagc gccaccggaa tcatgaacag
      241 gatggcggct atattccatt ccggcgtgac gaactgcggc atggcaatcc acggcgcttc
      301 agcgacttgc gaaaagtcga ccagccccag cagcacggcg gccaggtagc cggcaccgac
      361 gcccgccagg atcggcacca ggcgaaagaa gcccttggcc cataccgaga caccgatggt
      421 caccgcgagc gagatgccgg ccagaacgag cgaggtgccg tagctcatca attccgcctt
      481 gccgtcgccg gtcttgccca tcgccagatt gactgcgacg gcagcgagcg acaggccgat
      541 gatcatgatg atcggcccga tcactaccgg cggcatcatg cggtggatga agtcggcgcc
      601 gtgtctgcgc accagcgcgg cgagcacgaa gtacatcagg ctggcgcagg ccagcccgcc
      661 catcgtcgcc ggaacgcccc aggtctgcac cgaatagatg atcggggcaa tgaaggcgaa
      721 cgaggagcca aggaagatcg gcacctgccg ccctgtcagt gcctggaaga tcagcgtgcc
      781 gacaccggcg ccgagcaggg ctaggctggg atcgaggccg gtcaggatgg gaacgaggac
      841 gagggcgccg aaggcgacga agaggatctg cgcgccagag attgcgcggc gccagagcgg
      901 ttcggaggct gacattaggg ggttctctag gcgttgtgtt ggaaggtatt ccgacgggta
      961 cagtgcgccg gatggcccgg cgcacccggc gatcgattac ttggtgccga agatcttgtc
     1021 gccggcatcg cccaggccgg gaatgatgta gccgtgttcg ttgaggtgcg agtcgatcgc
     1081 cgccgtatag acgtgcacat ccggatgcgc cgcctccagt gcacgaatgc cttcgggcgc
     1141 ggcaaccagc accagcgcct tgatctgtcg ggcgcccttg cgcttgagca tgtcgatggt
     1201 ggcgatcagg gaaccgccgg tggcgagcat cgggtcgatg atcagcgcca ggcgctcgtc
     1261 gagcttgccc acgaaccgct cgaagtaggg taccggctgc agcgtctgct cgttgcgggc
     1321 gatgccgacg acgctgacct tggcgcccgg aatcagatca aggacgccat cgagcatgcc
     1381 cagtccggcg cgcaggatcg gcacgacggt gaccttcttg cccttgatct gctgcacttc
     1441 gaccggcccc gcccagccgg cgatggtgca cgcctcaagg tcgaaatctc gcgccgcctc
     1501 gtaggtaagc agccgagcca attcggcggt cagctcgcgg aatttcttgg tgctgatttc
     1561 ggcctcgcgc atgaggccga tcttgtgctg aaccagcggg tggtgaacgg caacgaccgg
     1621 catcgaggaa actccgaaaa aagcgcgcgc aaggttagcc gatttgggcg ttcgacgcac
     1681 gggcgccggc gcccgaaaga atcatccgac cgccgcaggc gccacgaaat aggtcgcgta
     1741 gaagaagtac gcggtgatgg cgacgctcgc gacgatgacg aagcggcgga cttgctgctg
     1801 cggcagcttg cgcgagacgt gcgcggcgac atagccgccg agcagcgtgc cggccagtac
     1861 gatcgtgcct tcgtaccagg caatcgcgcc gttggcgatg aagagcacta tggcaatcag
     1921 cgacacggcc gttgatacca gcagcttgag gccgttcatg gcgttgatgt tgctgtggcc
     1981 ggcgagcgcc aggtagctca gcgtgatgat gccgaggccg gcgttgaaga agccaccata
     2041 gacgcagacg gcgagcagca ggacggcgag caagatcgcg cccacggccg aggcgtggcg
     2101 gtggccggcg gcaagcgcct tcagttgctg attgatctgc ccgccgtaga tgaagagcac
     2161 ggtggcgaac agcagcagcc agggaatcgc cgcgcgaaac acggcctctg gcgtctgcag
     2221 caacagccag gcaccggcga tgccgccgag caggctgatt gcaaccaggc gcggcagctc
     2281 ggcgcggtgc gccaccagat cccggcgcag cgcatagacg ccgctcaggt agccggcgca
     2341 agaggcaaag gtgttggtgg cgttggcgct gatcggcgcg acgccgaccc acatcagtgc
     2401 cgggaaggtg atgaagctgc ctccaccggc aaccgaattg agcacgccgc cgaaaacgcc
     2461 ggctaggaac aggagcacga gttcgaggag catcggtctt ggctttggct ttcttccggc
     2521 cggtggcttg ggtcgattga ggcggacaaa tcgggtggcg cctgctcgtt tgcccgatgc
     2581 ggcgacggac tgcgagtatc ggcacgacga gtgaacaaga caagggcctg cgctgtggca
     2641 ggccccgtag gggtacggct atcgagtggc ttgcttagtg ccgcgaccgg ttgcggtctc
     2701 ggccacgacg gtcgtcccag cggtcgtcac cacgccgctt gttgccccag ccgcgacggt
     2761 catcgtggcg atcatggtcg cgccaggcgc ggcggccgtg gtcgtggcgg ttccagtggc
     2821 tgtggcggct ttcatggcga tgccggtgga ttacccgtac cggcggcggc acgacaatga
     2881 cgcggctgga gtggctgcgg ttttcatgac gatggccgcc gatgccgtag gtaatgttga
     2941 agccgtcgtg gagcaggctg ccgaccgaca gcgtcaggtg ttcgaccggg cgcaggacat
     3001 gcctctcgac atggccgaag tcggtggcga gggcgccggt gctagcggcg agcagggagg
     3061 cggcgagaag tgcttggcgg atcatggctg ttctccttgg tggctgttgt tgttggctta
     3121 gcggtcgtcg tcgatggaga ccgtgactcg taggttcacg aagcggcccg ggtcgtaggg
     3181 cagctgtgtg gcgtactcgc ggccgtcgta gcggtagcga acctggtagc cgctgatgac
     3241 ctggcggaca tcttcggtgc tgcggcaacg ttcgacgacg cgtgcgg
//
LOCUS       DATDAA010000320         6642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_23505,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000320 DATDAA010000000
VERSION     DATDAA010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23505"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VIS73_09820"
     CDS             <1..691
                     /locus_tag="VIS73_09820"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY5763489.1"
                     /translation="SRVRAFETGRCFLRGDAGETVPGYRQPRMLGILMAGTVATEQWG
                     ERDRQVDFYDLKGEVETLLQGQRAAFERVAHPALHPGRSAAVVVAGQRIGHFGELHPR
                     WVQQYELEAPPMLGELALDALLAADVPAYRVLANTPTVVRDLALVVAQDVAFVTIRAA
                     IEKHRAAIVRAVDLFDVYAGKGVPEGKKSLAFRVVMQDTEKTLQDSEVEEAVASLVRG
                     LSQTLGAELRS"
     gene            705..1019
                     /locus_tag="VIS73_09825"
     CDS             705..1019
                     /locus_tag="VIS73_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEY5763490.1"
                     /translation="MKMTLTKAELADILFEKVGLNKREAKDMVEAFFDEVRDALQRGE
                     GVKLSGFGNFQLRDKPQRPGRNPKTGEEIPITARRVVTFHASQKLKAEVETSYHGQRD
                     AS"
     gene            997..1365
                     /locus_tag="VIS73_09830"
     CDS             997..1365
                     /locus_tag="VIS73_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY5763491.1"
                     /translation="MDSATQAKVGKEDLPPIPAKRYFTIGEVSELCGVKPHVLRYWEQ
                     EFNQLKPVKRRGNRRYYQHHEVLLVRRIRELLYSEGFTISGARNRLESGEGAGESDGG
                     KLSFAELRNELLSIVRLLKA"
     gene            complement(1452..3050)
                     /gene="aceB"
                     /locus_tag="VIS73_09835"
     CDS             complement(1452..3050)
                     /gene="aceB"
                     /locus_tag="VIS73_09835"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEY5763492.1"
                     /translation="MTLKLPAGVQVNAPIHPRFDEILTHDALALVAKLHRAFNGRRHE
                     LLKKRVERQARIDAGEMPDFLPETKAIRDGDWKVAPLPKALECRRVEITGPVEAKMII
                     NGFNSGADSYMTDFEDSNSPNWFNQIQGQVNLFDAIRRKLVFKNEAGKEYRLNDKIAT
                     LQIRPRGWHLDEKHILVDGERVSGGIVDFGLVFFHNAKEQIARGAGPFYYLPKMESHL
                     EARLWNDIFVMAQEHIGLPKGTIKATVLVETILATFEMEEILYELRDHSSGLNAGRWD
                     YIFSCIKKFKKNKDFCLANRSAITMEVPFMRSYALALVKACHKRGAPAMGGMSALIPI
                     KNDPAANEKALAGIRRDKTRDANDGYDGGWVAHPGLVGIAMEEFVKVLGDRPNQWDKQ
                     RTDTYTAQDFLNFQPEKPITEDGLRNNINVGIHYLGSWLAGNGCVPIHNLMEDAATAE
                     ISRSQVWQWVVSPKGVLDDGRRVTVEMVRPMIAEELAKVKAEVSAQGENTETYDQAAV
                     IFDKMSLTPEYPEFLTLPLYEAMD"
     gene            complement(3136..3843)
                     /locus_tag="VIS73_09840"
     CDS             complement(3136..3843)
                     /locus_tag="VIS73_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEY5763493.1"
                     /translation="MANPTQDKPVIKAIAFDAYGTLFDVLSVGALAEQLFPGKGAELA
                     NLWRVKQIDYTRLRTLSDRYRDFYEVTRDALRMAARSLALELDAGTEQRLMNQYACLA
                     AFPENLGALKELKALGVPMAILSNGTPAMLEIAVKSAGMGAIFDHLLSVDSVRQYKTA
                     AAAYQLGPDAFGLPAAEILFVSSNCWDACGATWFGYTSFWINRANQPLEELGVTPNAT
                     GRLLTEVLNFVRTQAPA"
     gene            3982..4881
                     /locus_tag="VIS73_09845"
     CDS             3982..4881
                     /locus_tag="VIS73_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5763494.1"
                     /translation="MDIFKQIEAFVQVATRGGLSAAADGLGVTPAMVGRRLDALEARL
                     GVKLMQRTTRRMSLTFEGQAFLEDCQRILHELANAEASVSLGGVKASGHIRMTAPAGF
                     GRRHVAPLLRSFLATHAEVTVNLDLADRLVDLVNESIDCAIRVGELSDSNLVSIRLAE
                     NRRLVVASPAYLARRGQPQTPDDLARHNCLSLGEQRGWLFADRERPESGFTLKVAGNF
                     ECNDGAVLHEWALAGEGLAWRSLWEVGEDLAAGRLVPVLEDWATPPLGIYAVFPQRRY
                     LPLRVRLLIDFLKNTYGNPDYWT"
     gene            complement(4904..5116)
                     /locus_tag="VIS73_09850"
     CDS             complement(4904..5116)
                     /locus_tag="VIS73_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763495.1"
                     /translation="MPRSRLLYLTTMLAAAAVLLAACDRRAWYEGTQAGQRIQCMRLP
                     PAEARDCEARIPGSYEGYERERQGDR"
     gene            complement(5121..6002)
                     /locus_tag="VIS73_09855"
     CDS             complement(5121..6002)
                     /locus_tag="VIS73_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY5763496.1"
                     /translation="MTRFRLLPAVLGALALTACATSDGPYTPPLDDGRLPTALEMRQL
                     PGSLIGRGTRQFERVWLADPTSRYSHAVFGTTLNAESLLAVTRFGETYQVTLPPERVF
                     EDIEPRVIDLDRDALEEILVVESDRDKGASLAIYGTPIGAKQLVKLGATPFIGRAQRW
                     LHPLGAGDFDGDGWLDVALVETPHIGGSLVLYRYRQGRLSEFARFFGVSTHEIGSREL
                     DTARVVRGRLRDHLLTPDQSWHKLQLLTWRPTGIHRVKEWRLPARLRRIDPPQGGQVA
                     VVLADGQMLRLELPDLE"
     gene            complement(6030..6518)
                     /locus_tag="VIS73_09860"
     CDS             complement(6030..6518)
                     /locus_tag="VIS73_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEY5763497.1"
                     /translation="MAKKTRDIGIGIAEKDRARIAGGLNRLLADSFTLYLTTHNFHWN
                     VTGQQFNSLHLMFMDQYTELWNAIDPIAERIRALGFPAPGSYASFGKLTTLPDAPAKP
                     PKAMQMVENLARGHEAVAATAREVFVIAEAANDQPTADLLTQRMDIHEKTAWMLRAIL
                     KD"
BASE COUNT         1156 a         2008 c         2194 g         1284 t
ORIGIN      
        1 gagccgcgtc cgcgcgttcg agacgggccg ctgtttcctc cgtggcgatg ccggcgaaac
       61 cgtgccggga taccgtcagc cacgcatgct cggcatcctg atggccggca ccgttgccac
      121 cgagcagtgg ggcgaacgcg atcgtcaggt cgacttctac gatctgaagg gcgaggttga
      181 aacgctcttg cagggacaac gcgctgcctt cgagcgcgtt gcccacccgg cactgcatcc
      241 ggggcgatcg gcagcggtgg ttgttgccgg tcagcgtatt ggtcacttcg gcgaactgca
      301 cccgcgctgg gtgcagcaat atgaactcga ggcaccgccg atgctgggcg aactggcgct
      361 ggatgcgctg ctggctgctg acgtgccggc ttaccgcgtt ctggcgaaca caccgacggt
      421 ggtgcgcgat ctggccttgg tcgtcgctca ggatgttgcc ttcgtgacga ttcgtgcggc
      481 gatcgagaag catcgtgcgg cgatcgtcag ggcggtcgat ttgttcgacg tatatgccgg
      541 aaagggagtg ccagaaggga aaaaaagcct tgcgttccgc gtagttatgc aagatactga
      601 aaaaaccctt caggattctg aagtcgaaga agcggtcgcc tcgctggttc gcggattgag
      661 ccagacgctc ggcgctgagc ttcgctcgtg ataccagtca ggaattgaaa atgacgctca
      721 ccaaagcgga actggccgat attcttttcg agaaagtcgg cctgaacaag cgcgaggcga
      781 aggacatggt tgaggccttc ttcgacgagg tgcgcgatgc cctgcaacgt ggcgagggag
      841 tcaaactgtc cgggttcggc aacttccagc tgcgcgacaa gccgcagcgt ccggggcgca
      901 atcccaagac cggcgaggag attccgatca ccgctcgccg tgtcgtcacc tttcatgcca
      961 gccagaaact gaaggcggaa gtcgagacgt cctatcatgg acagcgcgac gcaagctaag
     1021 gtcggcaagg aagaccttcc gccgatcccg gccaagcgct acttcactat cggtgaggtc
     1081 agcgaactct gtggtgtcaa accgcatgtg ctccgttact gggagcagga gtttaatcag
     1141 ctcaaaccgg tcaagcgtcg gggcaaccgc cgctactacc aacaccatga agtcctgctg
     1201 gtgcggcgca tccgcgaact gctctacagc gaaggcttta cgatcagcgg cgcgcgcaat
     1261 cggctcgaat ccggggaggg cgcaggagaa tccgatggcg gaaaactgtc cttcgccgaa
     1321 ctgcgcaacg aactgctgtc catcgtccga ttgctgaaag cctgatcgct cgatttgttc
     1381 gtcggcaagc cggcgattcg aaccagacaa cggcgcccgc gggcgccgtt tccatgtgcc
     1441 ggtgagccga ctcagtccat cgcctcgtag agcggcagcg tgaggaactc ggggtactcc
     1501 ggcgtcaggc tcatcttgtc gaagatcacc gcggcctgat cgtaggtctc ggtattttcg
     1561 ccctgggcgc tgacttcggc cttcaccttc gccagttcct cggcgatcat cggtcgcacc
     1621 atctcgacgg tgacgcgccg gccgtcatcg agcacgccct tcggcgagac cacccactgc
     1681 cagacctgcg agcgagagat ctcggccgtt gccgcatctt ccatcaggtt gtggatcggc
     1741 acgcagccgt tgccggcaag ccatgagccg aggtagtgga tgccgacatt gatgttgtta
     1801 cgcaggccgt cctcggtgat cggcttttcg ggctggaagt tgaggaagtc ctgggccgtg
     1861 tacgtatcgg tgcgctgctt gtcccactgg ttggggcggt cgccgagtac tttgacgaac
     1921 tcttccatcg cgatgccgac caggcccgga tgcgcgaccc atccaccgtc gtagccatcg
     1981 ttggcgtcac gcgtcttgtc gcggcgaatg ccggccagcg ccttttcgtt ggccgctggg
     2041 tcgttcttga tcggaatcag cgcgctcatg ccgcccatcg ccggtgcgcc gcgcttgtgg
     2101 caggccttga ccagtgccag cgcatagctg cgcatgaacg gcacttccat cgtgatcgcg
     2161 cttcgattgg cgaggcagaa atccttgttc ttcttgaact tcttgatgca cgagaagatg
     2221 tagtcccagc gaccggcgtt gaggcccgag gagtggtcgc gcagctcgta aagaatttct
     2281 tccatctcga aggtggcgag gatggtctcg accagcaccg tcgccttgat cgtccccttc
     2341 ggcagtccga tgtgctcctg cgccatgacg aaaatgtcgt tccacaggcg tgcctcaagg
     2401 tgcgattcca tcttcggcag gtagtagaag ggaccagcgc cgcgggctat ctgctccttg
     2461 gcgttgtgga agaacaccag gccgaaatcg acgatgccgc cggagacgcg ctcgccgtca
     2521 acaaggatgt gcttctcatc caggtgccag ccgcgcggac ggatctgcag cgtcgcgatc
     2581 ttgtcgttga gcctgtattc cttgccggcc tcgttcttga agaccagctt gcggcggatc
     2641 gcgtcaaaga gattcacctg accctggatc tggttgaacc agttggggct gttcgaatcc
     2701 tcgaaatcgg tcatgtacga atcggcgccc gaattgaagc cgttgatgat catcttcgct
     2761 tcgacgggcc cggtgatctc gacgcggcgg cattcgagcg ccttgggcag tggcgcgacc
     2821 ttccagtcgc cgtcgcggat ggccttggtt tcaggcagga agtcgggcat ctcgccggcg
     2881 tcgattcggg cctggcgttc gacgcgcttc ttcagcagtt cgtggcggcg gccattgaag
     2941 gcgcgatgca gcttggcgac caaggccagc gcatcgtgcg tgaggatttc atcaaaacgc
     3001 ggatgaatcg gtgcgttcac ctgaacgccg gcgggcagct tgagagtcat ggtgtctcct
     3061 tgaagggggt tggtcagaga tgggtcttgt cagaataggt cggggtcagt ccgctgccgc
     3121 ccgtgtgtac ggccatcagg cgggcgcttg cgttcggacg aagttcagca cctcggtcag
     3181 caggcggccg gtggcattcg gcgtaacgcc gagttcctca agcggctgat tggcgcggtt
     3241 gatccagaag ctggtgtagc cgaaccaggt tgcaccgcag gcgtcccagc agttcgacga
     3301 cacgaagagg atctcggcgg ccggcagccc gaaggcgtct ggccccagtt gatacgcggc
     3361 ggcggcagtc ttgtattggc gcaccgagtc gaccgacagc aggtgatcga agatcgcgcc
     3421 cattccggcg ctcttgacgg cgatttcgag catcgctggc gtaccgttgg agagaatcgc
     3481 catcggcacc ccaagtgcct tcagttcctt cagtgcgcca aggttctcgg gaaaggcggc
     3541 gaggcaggcg tactggttca tcagccgctg ctcggtgccg gcgtcgagtt caagcgccag
     3601 cgagcgggcg gccatgcgca gtgcgtcgcg cgtcacttcg tagaaatctc ggtagcgatc
     3661 cgacagcgtc cgcaggcgag tgtagtcgat ctgcttgacg cgccacaggt tggcaagctc
     3721 ggctcccttg cccgggaaga gctgctcggc cagcgcgccg accgacaaca cgtcgaaaag
     3781 cgtgccgtag gcgtcgaagg cgatcgcctt gatcaccggt ttgtcttggg tggggtttgc
     3841 catcttgcct gcctcggtcg agctgccgcg gacggagcac gacagtaaat tgtctgtcgg
     3901 tgcattctag aacatatcgt taatgcgtta aacgaactat atttgccaac accttttaca
     3961 aaaagtatgt aatgggctgt catggatatc ttcaagcaga tcgaagcctt cgttcaggtt
     4021 gctacgcgcg gggggctgtc ggcggcagcc gatggcctcg gcgtcacgcc ggcaatggtc
     4081 gggcgccggc tcgacgcact cgaggcgcgg ctgggcgtca agctgatgca acggaccacg
     4141 cgcaggatgt cgctgacctt cgaagggcag gccttcctcg aggattgcca gcgcatcctg
     4201 cacgagctcg ccaacgctga ggcctcggtc agcctgggcg gcgtcaaggc aagcggacac
     4261 attcgcatga ccgcgccggc gggtttcggg cggcgccacg tcgcaccgct gctgcgctcg
     4321 ttcctcgcca cccatgccga agtgacggtg aacctcgact tggctgatcg cctcgtcgat
     4381 ctggtcaacg agagcatcga ttgcgcgatc cgtgttggcg agttgagcga ttcgaatctg
     4441 gtgtcgatcc gtcttgccga gaaccgccgc ttggtcgtcg ccagcccggc ctatctggcg
     4501 cgtcgcggcc agccgcaaac gccggacgat ctggctcgcc acaactgcct gtcgctgggc
     4561 gagcagcgcg gttggttgtt tgccgatcgc gagcggccgg aaagtggctt cacgctcaag
     4621 gttgccggca atttcgagtg caacgacggt gccgtattgc acgagtgggc cctggccggc
     4681 gaaggcttgg cctggcgttc gctgtgggaa gtcggcgagg acctggcagc cgggcggctg
     4741 gtgccggttc tcgaggactg ggcgacgcca ccgttgggta tctatgcggt gtttccgcag
     4801 cgccgctacc taccgctgcg ggtccgactg ctcatcgatt tcctaaagaa cacctacggc
     4861 aatcccgact actggacctg aggcgacggg agcgggagcc gcgctaccgg tcgccttgcc
     4921 gctcgcgttc gtagccctcg tagctgccgg gaattcgggc ttcgcagtcg cgggcctcgg
     4981 ccggcggcag gcgcatgcac tggatgcgct gcccggcctg cgtgccctcg taccaggcgc
     5041 ggcggtcgca ggcagcaagc agcacggcgg cggctgccag catggtggtg aggtaaagca
     5101 gacgcgatcg cggcatcgcc ttattcgagg tccggcagct caaggcgcag catctggccg
     5161 tcggcaagca cgaccgccac ttgcccaccc tgaggcgggt cgatgcggcg cagccgggca
     5221 ggcaggcgcc attccttgac gcggtggatg ccggtcggtc gccaggtcag caactgcagc
     5281 ttgtgccagc tctgatcggg agtcagcagg tgatcgcgca ggcggccgcg aacgacgcgt
     5341 gcggtatcga gttcgcggct gccgatctcg tgcgtggaca cgccgaagaa gcgcgcaaac
     5401 tcggacaagc gcccctggcg gtagcggtag agcacgaggc tgccgccgat gtgcggggtt
     5461 tcgaccagtg cgacgtcgag ccagccgtcg ccatcgaaat cgccggcgcc gagcggatgc
     5521 agccaacgct gggcgcggcc gatgaagggg gtggcgccca gcttgaccag ttgcttggcg
     5581 ccaattggcg tgccgtaaat cgccagcgag gcgcccttgt cgcggtcgct ctcgacgacg
     5641 aggatctctt cgagtgcgtc gcggtcgagg tcgattacgc gcggctcgat gtcttcgaag
     5701 acacgctcgg gcggcagcgt cacctggtag gtctcgccaa agcgggtcac cgccagcagc
     5761 gattccgcgt tcagcgtagt accgaaaacc gcgtgcgagt agcggctcgt tggatcagcg
     5821 agccagaccc gctcgaactg ccgcgtgccg cgtccaatca ggctgcccgg cagctggcgc
     5881 atctccagcg cggtcggcag gcgaccgtcg tcgagcggcg gcgtgtacgg gccgtcgctg
     5941 gtggcgcagg ccgtcaacgc cagcgcgcca agcacggccg ggagcaggcg aaagcgcgtc
     6001 acgcgtggct cttcacggcg ctgctcccct cagtccttga ggatggcgcg cagcatccac
     6061 gccgtcttct cgtgaatgtc catgcgctgg gtcagcaagt cggcggtcgg ctggtcgttg
     6121 gcggcttcgg cgatcacgaa tacctcgcgc gccgtcgcgg caacggcttc atgaccgcgc
     6181 gccagattct cgaccatctg catcgccttg ggtggcttgg ccggtgcatc gggcagcgtg
     6241 gtcagcttgc cgaaggatgc gtaggaaccg ggggccggaa atccaagcgc gcgaatgcgt
     6301 tcagcgatcg ggtcgatggc gttccacagt tcggtgtatt gatccatgaa catcaggtgc
     6361 agcgaattga actgctgacc ggtgacgttc cagtggaaat tgtgggtggt cagatagagc
     6421 gtgaatgagt cggcgagcag gcgattaagc cccccggcaa tgcgcgcgcg atccttctcc
     6481 gcaatgccga tcccgatgtc ccttgttttc ttggccatgc tgttctcctt ttcgatacca
     6541 gttgttctta gatgagctgg cttgccgtgg tcatcgtagc aaacccgcct gcgaaatagc
     6601 agccggcggg ccaactagcc agcgtccgga ccgcagtcgg ca
//
LOCUS       DATDAA010000321         3829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_443527,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000321 DATDAA010000000
VERSION     DATDAA010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443527"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1354
                     /locus_tag="VIS73_09865"
     CDS             44..1354
                     /locus_tag="VIS73_09865"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY5763498.1"
                     /translation="MKPINVGLLGIGTVGGGTFSVLSRNASEITRRAGRPIQITVVAD
                     RDTARAKQLTAGSCRVTDDAFAVVSDPEVDIVVELIGGYSVARELVLKAIANGKHVVT
                     ANKALLAIHGTEIFAAAQKHGVMVAFEAAVAGGIPIVKAVREGLTANRIEWIAGIING
                     TTNFILSEMRDKGLAFETVLKEAQALGYAEADPTTDIEGFDAAHKITILSALAFGIPV
                     QFDKAHVEGISKLDATDIRYAEQLGYRIKLLGIAKRRADGIELRVHPTLIPAKRLIAN
                     VEGPMNAVVVSGDAVGTTLYYGKGAGAEPTASAVIADLVDVTRMHTADPEHRVPHLAF
                     QPDALSNLPILPMAEIETGYYLRLRVADKTGVLADITRILADQQISIDAMIQREPAEG
                     EDQTDIILLTHVTREKQIDAAIVRIEALEAVLGKLTRLRCEELQ"
     gene            1369..2526
                     /locus_tag="VIS73_09870"
     CDS             1369..2526
                     /locus_tag="VIS73_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763499.1"
                     /translation="MSPLTAGLAAVAAFVVAAILAASGAPQALVAHLVFAVGVVPLVF
                     AAILHFVPVLTRTGAPSSTLALLPWAAQAAGGLAVLAFAGWMPRGALSIAAAIDLLLA
                     GVLALWILRRRGAALGGAHPGWRWYLAALAALMVALAAIAAIELFPEAFRSLRALHIH
                     LNTLGFLGLAAFGTLPVLLPTAVRRPDPGARRWLAACLPLVATGAALVAVGAALSTAT
                     IPFDLPLIAAIVGAVLLVSAALALLWRWSSAYGVTAIAGGGAASSLAAAVAGFVVLLP
                     IGVLHAFPVLAQGAAVAAFGGLFLLPLVSGALSQLLPVWRFPGVDTVARQRLAWVLGS
                     WSRLRVACFLSGGVLFALGRADWAIAVVATGLLLLLGVTLVGLNESRAGGR"
     gene            complement(2498..3175)
                     /locus_tag="VIS73_09875"
     CDS             complement(2498..3175)
                     /locus_tag="VIS73_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5763500.1"
                     /translation="MDRFATMPWHRQWVARVRVDFWLKGVGITAFIWGFFVAYFWLLR
                     NPFFPVAVMPTTPPDDWISFAPASLGLYLTLWVYVTFPPSLIVDRAQLVRYGIAAALL
                     CGTGLLIFLFWPSAVPAGLVSEAEQLSAGFALLKGIDAAGNAFPSLHVATAVFSAFWL
                     DRLLRQMNAGVLVRGINVAWCCGIVYSTLATKQHVFLDALSGTILGIVAAWLSLAWIS
                     ARPPGIR"
     gene            3290..>3829
                     /locus_tag="VIS73_09880"
     CDS             3290..>3829
                     /locus_tag="VIS73_09880"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927113.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY5763501.1"
                     /translation="MRYLSTRGHSAPQRFCDILLGGLAPDGGLYLPEVYPQIRRSELD
                     AWRHLPYADLAFAILSKFIDDIPAEQLRDICQRTYSAETYCHSRSPELVAEITPVRWL
                     EDGELGLLELSNGPTLAFKDMAMQLLGNLFEHVLEARGERINILGATSGDTGSAAEYA
                     MRGKQRVRVFMLSPEGKMSA"
BASE COUNT          619 a         1281 c         1266 g          663 t
ORIGIN      
        1 ggtacgtgac cggcacaacc ccgctcagga gactcagtta cacatgaaac cgatcaacgt
       61 aggcttgctg ggcattggca ccgtcggcgg cggcaccttc tccgttcttt cgcgtaacgc
      121 aagcgagatc acccgccgcg ccggacggcc gatccagatc accgtggtcg ccgaccgcga
      181 cacggcgcgg gccaaacaac tgaccgccgg cagctgtcgt gtcaccgacg atgcctttgc
      241 ggtggtcagc gaccccgagg tggatatcgt cgtcgaattg atcggcggct attcggttgc
      301 ccgcgaactc gtgctcaagg ccatcgccaa cggcaagcac gtggtcacgg ctaacaaggc
      361 gttactggcg atccacggca ccgagatctt cgccgccgcg cagaagcacg gcgtgatggt
      421 tgccttcgag gcagcggtcg ccggcggcat ccccatcgtc aaggcggttc gcgagggctt
      481 gaccgcgaac cgtatcgagt ggatcgccgg catcatcaac ggcacgacca acttcatcct
      541 ttccgaaatg cgtgacaagg gcctcgcctt tgagaccgtg ctcaaggaag cgcaggcgct
      601 cggctatgcc gaagccgacc cgacgaccga catcgaaggc ttcgacgccg cgcacaagat
      661 cactattctc tcggcgctcg ccttcggcat cccagtgcag ttcgacaagg cgcacgtgga
      721 gggcatcagc aagctcgacg ccacggatat ccgctacgcc gagcaactcg gttatcgtat
      781 caagctgctc ggcatcgcca agcggcgcgc cgatgggatc gaactgcgcg ttcacccgac
      841 gctgattccg gcgaagcgcc taatcgccaa tgtcgagggc ccgatgaacg cggtcgtcgt
      901 cagcggcgac gcggttggca cgacgctcta ctacggcaag ggcgccggcg ccgagccgac
      961 cgccagcgcg gtcatcgccg atctggtcga cgtcacccgt atgcatacgg ccgaccccga
     1021 gcaccgcgtg ccgcacctgg ccttccagcc cgacgccttg tcgaacctgc ccatcctgcc
     1081 gatggccgag atcgagaccg gttactacct gcgcctgcgc gtcgccgaca agaccggcgt
     1141 gctcgccgac attacccgca tccttgccga ccagcagatt tcgatcgatg cgatgatcca
     1201 gcgcgagccg gccgagggcg aggaccagac cgacatcatc ctgctcacgc acgtgacccg
     1261 ggagaagcag atcgacgctg cgatcgtccg aatcgaggca cttgaggccg ttctcggcaa
     1321 gctcacgcgg ctgcgctgcg aagaactgca gtaagcggcc ctgcggccgt gtcgcccctg
     1381 acggcgggac tcgcggccgt tgccgccttc gtagtggcgg cgattctcgc cgcgagcggc
     1441 gcgccgcagg cgctcgtcgc ccaccttgtg ttcgcggtcg gcgtcgtgcc actggtgttc
     1501 gcggcgatac tgcatttcgt accggtgctg acccgcaccg gcgcgccgtc gtcgacgctg
     1561 gcactgctcc cctgggctgc acaggcggcc ggaggactcg ccgttctggc gttcgccggc
     1621 tggatgccgc gcggcgccct gagcatcgcc gccgcgatcg acctgctgct ggccggcgtg
     1681 ctggccttgt ggattcttcg ccgccgcggc gcggcgctcg gcggtgccca tccgggctgg
     1741 cgctggtact tagcggcact tgccgccctg atggtcgcgc ttgccgcaat cgcggctatc
     1801 gaactttttc ctgaggcatt ccggtcgctc agggcgctgc acatacacct caacacgctc
     1861 ggctttctcg gtctggccgc cttcgggaca ctgccggtcc tgctgccgac ggccgtgcga
     1921 cggcccgacc ccggcgcacg gcgctggctt gccgcgtgcc tgccgctggt ggcgacgggc
     1981 gctgcgctgg ttgccgtcgg cgcggcgctg agcacggcga cgatcccctt cgatctcccg
     2041 cttattgcgg cgatcgtcgg cgcggtgctg cttgtttcgg ccgcgctcgc gctgctctgg
     2101 cgctggagca gcgcgtatgg cgtcactgcg atcgccggcg gcggtgcagc cagctcgctg
     2161 gcggcggcgg tagcgggatt cgtcgtgctg ctgcccatcg gcgtgctgca tgcgttcccg
     2221 gtgcttgccc agggcgccgc agtcgcggcc ttcggtgggc ttttcctgct gccgctggtc
     2281 agcggggcac tgagccagct tttgcctgta tggcgttttc ccggcgttga cacggtcgca
     2341 cggcagcgcc tggcatgggt actggggagt tggagtcgcc tgcgcgtcgc ctgtttcttg
     2401 agcggcggcg tgctgttcgc gcttgggcgc gccgactggg cgatcgcggt ggtcgcgacc
     2461 ggcctgctgc tactgctcgg cgtgacgctg gtggggctca acgaatcccg ggcgggcggg
     2521 cgctgatcca ggccagcgac agccaagcgg ccacgatgcc gaggatcgtt ccggagagcg
     2581 cgtcgagaaa gacgtgctgc ttggtggcaa gtgtcgagta gacgattccg caacaccagg
     2641 cgacgttgat gccgcgcacg agaacgccgg cattcatttg ccgcaacagc cggtcgagcc
     2701 agaaggcaga aaacaccgca gtcgccacat gcagcgacgg gaatgcgttg ccggccgcat
     2761 cgatcccctt gagcagcgca aagccggccg acagctgctc ggcttcggag accagtcctg
     2821 ccggcaccgc gcttggccag aacaggaaga tcagcaggcc tgtcccgcag agcagggccg
     2881 cggcaatgcc atagcggacc agttgtgcgc gatcgacgat cagtgatggc gggaaagtga
     2941 cgtaaaccca gagcgtcagg tagaggccta gcgatgccgg cgcgaagctg atccagtcgt
     3001 cggggggtgt cgtcggcatg accgccaccg gaaagaaggg attccttagc agccagaagt
     3061 aggcgacgaa gaatccccag atgaaggcgg tgatgccaac gcccttcagc cagaagtcga
     3121 cgcggacgcg ggcgacccat tgccgatgcc agggcatcgt cgcgaagcgg tccactggca
     3181 gggaagggtg atggctggcg ggaggcattg ccgggactgc tgcggctgtt ggcgagggcc
     3241 gccatgataa ccttgcggcc ctttgtaatc cgcgattcaa cggcctctca tgcgctatct
     3301 atcgacccgc gggcactcag ccccacagcg tttctgcgac atcctgctcg gcggcctggc
     3361 gcccgatggc ggcctctatc tgcccgaggt ttatccgcag atccgtcgct ccgaactcga
     3421 cgcgtggcgg cacttgccct atgccgacct ggcgttcgcg atcctctcca agttcatcga
     3481 cgacattccg gccgaacagc tgcgtgatat ctgtcagcgc acctacagcg ccgagaccta
     3541 ttgccacagc cgttcaccgg aattggttgc cgaaatcacg ccggtgcgct ggctggagga
     3601 tggcgaactc ggcctgctcg agctctccaa cggtccgacg ctggcgttca aggacatggc
     3661 gatgcagctt ctcggtaacc tcttcgagca tgtgctcgag gcgcggggcg agcgcataaa
     3721 catcctcggc gccacctcgg gtgacacggg gtcggccgcc gaatacgcga tgcgcggcaa
     3781 gcagcgcgtg cgcgtcttca tgctctcgcc cgaagggaag atgagcgct
//
LOCUS       DATDAA010000322         7873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_58473,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000322 DATDAA010000000
VERSION     DATDAA010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7873
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58473"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /gene="trpB"
                     /locus_tag="VIS73_09885"
     CDS             <1..1004
                     /gene="trpB"
                     /locus_tag="VIS73_09885"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY5763502.1"
                     /translation="SRLTERLGGARIYLKREDLCHTGAHKINNTVGQALLARRMGKPR
                     VIAETGAGQHGVATATVAARYGMECIVYMGAEDIKRQAANVYRMKLLGATVVPVTSGS
                     KTLKDALNEAMRDWVTNVENTFYIIGTVAGPHPYPMMVRDFQSVIGDECIEQMPELIG
                     KQPDYAVACVGGGSNAMGLFYPYLDRPEVRLVGVEAAGDGIASGRHAASLTAGRPGVL
                     HGNRTYLLQDADGQIIETHSISAGLDYPGVGPEHAWLKDSGRASYESVDDAEALKAFH
                     DLCRFEGIIPALESSHALAYAARLAPTLPVGKSILVNLSGRGDKDMHTVAEKSGIVF"
     gene            1007..1816
                     /gene="trpA"
                     /locus_tag="VIS73_09890"
     CDS             1007..1816
                     /gene="trpA"
                     /locus_tag="VIS73_09890"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY5763503.1"
                     /translation="MSRIDSTFAALRAQGRKAFIPFVTAGDPDPALTVAVMQALADNG
                     ADVIELGVPFSDPMADGPTIQRASERALAKGMTLARVLAIASEFRAANPGIPVVLMGY
                     ANPIEAMGLAKFGQAAKAAGVDGVLVVDYPPEESEAFAACMTENGLDPIFLLAPTTTD
                     ARMAQIAAVARGYIYYVSLKGVTGSGTLDLGEIAGKVASIRAQSGLPVGVGFGIRDAR
                     TAAAIAAIADAVVVGSRLIEAMESAGHDRALEAVAALAAEIRAGVDTASKG"
     gene            1821..2687
                     /gene="accD"
                     /locus_tag="VIS73_09895"
     CDS             1821..2687
                     /gene="accD"
                     /locus_tag="VIS73_09895"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY5763504.1"
                     /translation="MSWLQKLLPPKIKREGKERRNVLPEGLWSKCQACEAVLYASDLK
                     QNHNVCPKCSFHIRLRARDRLDLLLDADGRFEIGAEVLPVDPLKFKDSKRYAERLMDA
                     AESTGETDSLVVMQGAIETLPVVVAAFDFEFMGGSMGSVLGERFVRGVNTALEQQLPF
                     VCVTASGGARMQEGLFSLMQMAKTTASLTRLAESRLPYISVLTDPTMGGVSASFAFVG
                     DVVIAEPGALVGFAGPRVIEQTVREKLPEGFQRAEFLLEKGAIDMIVDRRELRGRLAQ
                     LISTLMKLPAPN"
     gene            2734..4047
                     /gene="folC"
                     /locus_tag="VIS73_09900"
     CDS             2734..4047
                     /gene="folC"
                     /locus_tag="VIS73_09900"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEY5763505.1"
                     /translation="MSKPQSLAQWLAHIEALHPRGQQGIELGLDRVAEVARRLGQRPF
                     CPVITVGGTNGKGSVCAYLESILRRGGFRVGCYTSPHLLAYNERIRVDSAPATDEAIC
                     AAFARVDAARAGSARIGLTYFEFGTLAAWEVFAGADLDVIILEVGLGGRLDAVNIYDA
                     DLAILTSVGIDHAEYLGPDRESIGREKSGIFRAGVPAIVADGAPPASVVDAATAVGAD
                     LRLIGRDFGFLRSLENKAQWTFWMRREGELRRRPMAYPGIRGSIQLANAAAAVAGLDC
                     LRERLPVGMQAIRQGLIETELAGRFQVIPGQPALVIDVAHNPQAAQVLDANLGEMGFF
                     PVTTAVVGMMADKDIEGSLRPLIGRVDRWCCASLPGTRGASAETLAEVVRRLGGKEVH
                     AFDSVAAALDFAREKSGEADRIAAFGSFVTVAAVLAERDRRSACG"
     gene            4040..4672
                     /locus_tag="VIS73_09905"
     CDS             4040..4672
                     /locus_tag="VIS73_09905"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY5763506.1"
                     /translation="MAEQENDLELKKRARRRLVGAVAFAVLAVALLPTVMDEEPPRVA
                     SDIAIRIPGQDEARPVPLTATPESRPAPPVAPADPPPPSASADDDSPKAERPKVESPS
                     SEKPKPAVKPALAPAEVDAKRAQSILEGRPNEQEYVLLIGAYANMANVTALTGKLSGL
                     GIKTYTESVTLPQGKRTRVRAGPFETRAAAEAARDKMKRIGVSGQIAVRP"
     gene            4680..5171
                     /locus_tag="VIS73_09910"
     CDS             4680..5171
                     /locus_tag="VIS73_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY5763507.1"
                     /translation="MTVFDFVVLAVIVISAAFGGWRGFAGELLALAAWVLAVVAAWMF
                     GEEIGQAVFGNLIDDVSMRLVAGFVAVAVLVLFLAGLIRFAVREFIKAVGLTPTDRAL
                     GVLFGLSRGLAIVVVLVALGGLTPMPKEKWWRQAVLSAPTETLVVALKPWLPDGLARR
                     IRF"
     gene            5188..6732
                     /gene="purF"
                     /locus_tag="VIS73_09915"
     CDS             5188..6732
                     /gene="purF"
                     /locus_tag="VIS73_09915"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY5763508.1"
                     /translation="MCGILGAVANSGVNQLLYDGLMVLQHRGQDAAGIATAEGSTFNL
                     HKGPGLVRDVFRTRNMRALPGGMGIAHCRYPTAGSAFDFAEAQPFYVNSPFGIMLGHN
                     GNLTNSDSLREEMFRQDRRHINTNSDSEVLLNVLAHELQDRARGTQLDVDTIFAAVQG
                     VHRRCRGAYAVVAMIAGYGLLAFRDPHGIRPMVFGRNDAADGTEYLVSSESVALDTLG
                     FELERDVAPGEAIFIDLDHRLHSRQCADRPVLSPCIFEYVYLARPDSVMDGVSVYETR
                     LRMGEHLAEKVRRVLGPEQIDVVIPIPDSSRPAAMQLAQALGLSYREGFVKNRYIGRT
                     FIMPGQSTRRKNVRQKLNTVDREFEGKRVLLVDDSIVRGTTSREIVQMARDAGATRVY
                     FASAAPPVRYPNVYGIDMPTLAELIATGRTDAQIAAEIGADALVYQDLDALKAAVRGS
                     NPALSGFDASCFDGRYITGDVSAEYLAEIELRRGGASHSAASDGNSSRQLMLSLPDED
                     ELGEAA"
     gene            6729..>7873
                     /locus_tag="VIS73_09920"
     CDS             6729..>7873
                     /locus_tag="VIS73_09920"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEY5763509.1"
                     /translation="MSKGLPEGLHLDSLAVRAGQERSQFNEHSEALYLTSSFVFDSAA
                     QAAARFSGDEDGNVYSRFTNPTVTMFEERLAALEGAEDCVATASGMSAILSLVMAHLK
                     AGDHIVASGSLFGATVQMFTNLLPRFGIETSFVAQSRPDDWQAALRPNTRLLFVETPS
                     NPLTEIADIGALVAIARAHGALLAVDNCFCTPALQRPLDLGADLVIHSATKYLDGQGR
                     VLGGAVCGRKALVEEVFKFLRTAGPTLSAFNAWVLLKGIETLGLRMAAQSRAALELAR
                     WLEAHPKVGRVYYPGLDSHPQHALASRQQKSGGAILGFEVQGGRSEAWRVVDHCRLLS
                     ITANLGDVKTTLTHPATTTHGRISAEARTAAGISESLLRVAVGLEALS"
BASE COUNT         1298 a         2485 c         2648 g         1442 t
ORIGIN      
        1 cgtcccgcct gacggagcgg ctcgggggag cgcggatcta cctcaagcgg gaggacctct
       61 gccacaccgg tgcgcacaag atcaacaaca ccgtcggtca ggcgctgctg gcgcggcgca
      121 tgggcaagcc gcgggtaatc gccgaaaccg gtgcaggcca gcatggcgtc gcgacggcaa
      181 ccgttgccgc gcgttacggc atggaatgca tcgtctacat gggcgccgag gacatcaagc
      241 gccaggccgc caacgtctat cgcatgaaat tgcttggcgc gaccgtggtt cccgtgacct
      301 cgggttcgaa aacgcttaag gatgcgctga acgaagcgat gcgcgattgg gtgaccaacg
      361 tcgagaacac cttctacatc atcggcaccg tcgccgggcc gcatccctat ccgatgatgg
      421 tccgcgattt ccagtcggtg atcggtgacg agtgcatcga gcagatgccc gaactgatcg
      481 gcaagcaacc ggattacgca gtggcttgcg tcggcggcgg ctcgaacgca atgggcctgt
      541 tctaccccta cctcgatcgt cccgaggttc gtctggttgg cgtcgaggcg gcgggcgacg
      601 ggatcgccag cgggcgccac gcggcgtcgc tgaccgctgg ccggcccggc gtcctccacg
      661 gcaaccgcac ctatctgctg caggacgcgg acgggcagat catcgagacg cactcgattt
      721 cggccggtct ggattatccc ggcgtcggtc ccgagcacgc ttggctcaag gattccggcc
      781 gggcgagcta cgaatcggtc gacgacgccg aggcgctcaa ggcctttcac gatctctgcc
      841 gattcgaggg gatcattccc gcgcttgagt cgagccatgc cttggcgtac gctgcgcgcc
      901 tcgcaccgac gctgccggtg ggcaagagca ttctcgtcaa tctgtccggc cgcggcgaca
      961 aggacatgca caccgtggcc gagaaatccg gaatcgtctt ctgaccatgt cgcgaatcga
     1021 ctcaactttc gccgccttgc gggcgcaagg gcgaaaagcc tttatcccct tcgtcaccgc
     1081 cggtgatccc gacccggcct tgaccgtcgc ggtgatgcag gcgcttgccg ataacggtgc
     1141 cgacgtgatc gagttgggcg taccgttttc cgacccgatg gccgatggcc cgacgatcca
     1201 gcgcgcgtcg gagcgggcgc tggccaaggg tatgacgctc gcgcgcgtgc tcgccatcgc
     1261 cagcgaattc cgcgctgcca atccggggat tccggtggtg ctgatgggct acgcgaatcc
     1321 gatcgaggcg atgggcttgg ccaaattcgg ccaagccgcg aaggcggccg gcgtcgacgg
     1381 cgtgctcgtg gtcgattacc cgccagagga atcagaagcc tttgctgcct gcatgaccga
     1441 aaacgggctt gacccgatct tcctgctggc gccgacgacg accgatgcgc gaatggcgca
     1501 gatcgctgcc gtggcgcgcg gatacatcta ctatgtctcg ctcaagggcg tgacagggtc
     1561 cggcaccctc gacctcggcg agattgccgg taaggtcgcg tcgattcgcg cgcagagtgg
     1621 tttgcccgtc ggcgttggct ttggcatccg cgacgccagg accgccgcgg cgatagccgc
     1681 aattgctgac gcggtcgtgg tcggcagccg attgattgaa gcgatggaaa gtgccggtca
     1741 cgaccgcgcg ctcgaagccg tcgcagcact ggcggcggaa atacgagccg gtgtcgatac
     1801 agcaagcaag gggtgagacg atgagctggt tacaaaaatt gttgccgccg aagatcaagc
     1861 gcgagggcaa ggaacggcgc aacgtgctgc ccgaaggcct gtggagcaag tgccaggctt
     1921 gcgaggcggt gctctacgcg tcggacctca agcaaaacca caacgtctgc cccaagtgca
     1981 gctttcatat ccgcctgcgt gcgcgcgatc gtctcgatct gctgcttgat gccgacggcc
     2041 gtttcgagat cggagccgag gtgctgccgg tcgaccccct caagttcaag gacagcaagc
     2101 gctacgccga acgactgatg gatgccgccg agtcaaccgg cgaaaccgat tcgctggtgg
     2161 ttatgcaggg cgcgatcgaa accttgccgg tcgtcgtcgc tgcctttgat ttcgagttta
     2221 tgggtggctc aatgggctcg gtccttggcg agcggtttgt gcgtggcgtc aatacggccc
     2281 tcgaacagca gcttcccttt gtttgcgtca cggcttcggg cggcgcgcgc atgcaggagg
     2341 gcctgttctc gctgatgcag atggcgaaga ccacggcttc gctgacgcgg ctcgccgagt
     2401 cgcgcctgcc ctacatttcg gtgctcaccg atccgaccat gggtggcgtc tccgcttcgt
     2461 tcgccttcgt cggcgatgtg gtgattgccg agcccggcgc gctggtcggc ttcgccggcc
     2521 cgcgcgtgat cgagcagacg gtacgcgaga agctgcccga gggctttcag cgcgcagagt
     2581 tcctgctcga gaagggcgcg atcgacatga tcgtcgatcg tcgcgaactg cgcgggcggc
     2641 tcgcccagct catctccacc ctcatgaagc tgccggcgcc caattgacgc agcgtagttc
     2701 gcccgcgcgg cgaacgaggc agtagggttc cccatgtcca agccacaatc gctggcgcaa
     2761 tggctggcgc acatcgaggc tctgcaccca cgcgggcagc aaggcattga gctcgggctc
     2821 gaccgcgttg ccgaagtcgc gcggcggctc ggacagcgac cgttctgccc ggtgatcacg
     2881 gttggcggta ccaacggcaa gggctcggtc tgcgcctatc tcgaatcgat tctccggcgt
     2941 ggcggatttc gcgttggctg ctacacgtcg ccgcatctgc ttgcctacaa cgagcgcatt
     3001 cgcgtcgatt cggcgccggc gaccgatgag gcgatctgcg ccgccttcgc gcgagtcgac
     3061 gcggcgaggg ccgggtctgc gcgaatcggg ctcacctact tcgagttcgg gacgctggcg
     3121 gcctgggagg ttttcgcggg ggcggatctc gatgtgatca ttcttgaggt cgggctaggc
     3181 ggccggctcg acgccgtcaa catctacgat gcagatctgg cgattctcac cagcgtcggc
     3241 atcgaccacg ccgagtacct cggcccggac cgcgagtcga tcggccgcga aaagtccgga
     3301 atttttcgcg ccggggttcc ggcgatcgtc gccgacggcg cgccgcccgc gagcgttgtc
     3361 gatgccgcaa cggcggtcgg tgcagacctg cgcctgatcg ggcgcgactt cggttttctg
     3421 cgttcgctcg agaacaaggc gcagtggacc ttctggatgc gccgcgaggg cgagctgcgg
     3481 cggcgaccga tggcctatcc gggaatccgc ggcagcatcc agctcgccaa tgcggcggcc
     3541 gcagtcgcgg gtctagactg ccttcgcgag cggctgccgg tggggatgca ggcgatccgt
     3601 caggggctga tcgaaaccga actcgccgga cgctttcagg tgattcccgg gcagcctgca
     3661 ctggttattg atgtcgccca caacccgcag gcggcccagg tgctcgacgc caatctcggc
     3721 gagatgggct tctttccagt gacgaccgcc gtggtcggga tgatggccga caaggacatc
     3781 gagggcagct tgcggccgtt gatcggccgc gtcgatcgct ggtgctgcgc cagcctgccc
     3841 gggactcgcg gggcgagcgc ggagacgctt gccgaggtcg tccgccggct cggcggcaag
     3901 gaggttcacg ccttcgattc ggtcgctgcg gcgctcgatt tcgcacggga gaaaagcggc
     3961 gaagctgata gaatcgcggc cttcggttca tttgtaacgg tagcggcggt tctcgcagag
     4021 cgcgaccgac ggagtgcgtg tggctgagca ggaaaacgat ctcgagttga aaaagcgcgc
     4081 gcgccgccgt ctagttggcg ccgttgcctt cgcagtgctg gccgtcgcct tgctgccgac
     4141 ggtgatggac gaggagccgc cgcgcgttgc cagcgatatc gcgatccgca taccggggca
     4201 ggacgaagca cgtcccgtac cgctgaccgc gacgcccgaa tcgcgcccgg cgccgcccgt
     4261 tgccccagcc gaccccccgc cgccatcggc cagcgccgac gatgatagtc cgaaggccga
     4321 acggccgaag gtggagtcgc caagcagcga gaaaccgaaa cctgctgtga aaccggcgct
     4381 tgcgccggcc gaggtcgatg ccaagcgcgc gcagtcgatt ctcgaagggc gccccaacga
     4441 gcaggagtac gtcctgctga tcggcgccta cgccaacatg gccaacgtga ctgcgctgac
     4501 cggcaagctg tccgggctgg gcatcaagac ctacaccgaa tcagtaacgt tgccgcaggg
     4561 caagcgtacc cgcgtgcgtg ccggcccgtt cgagactcgt gctgccgccg aggccgcccg
     4621 cgacaagatg aagcggattg gtgtcagcgg acagatagcc gtgcggccct gacgaggcga
     4681 tgacggtatt cgatttcgtg gtgctggcgg tcatcgtgat ttcggcagca ttcggaggtt
     4741 ggcgtggttt tgccggcgaa ctgctggcgc tcgcggcctg ggtgctggcg gtcgtggcgg
     4801 cctggatgtt cggcgaggaa atcgggcagg ctgtttttgg caatctgatc gacgacgtgt
     4861 cgatgcggct ggtcgccgga ttcgttgccg tcgccgtgct cgtgctgttt cttgccgggc
     4921 tgatccgctt tgccgtgcgt gaattcatca aggcggtcgg gctgacgccg accgatcggg
     4981 cgctgggtgt attgttcggg ctgtcgcggg ggctggcaat cgtcgtggtt ctggtggcgc
     5041 ttggcgggct gacgccgatg cccaaggaaa aatggtggcg ccaggccgtg ttgtcggcgc
     5101 cgaccgaaac actggtcgtg gcactgaagc cgtggttgcc ggatggtctg gctaggcgga
     5161 tcaggttctg aagaggcggg tctccttatg tgcggaattc tcggtgcggt agcaaactcg
     5221 ggcgtcaatc agttgctcta cgacggcttg atggtcctcc agcatcgcgg tcaggacgcg
     5281 gcggggatcg ccaccgccga aggcagcacc ttcaacctgc acaagggtcc gggactcgtc
     5341 cgcgatgtct ttcgcacgcg caacatgcgc gcactgccgg gcggcatggg cattgcacac
     5401 tgccgctatc cgactgccgg ttcggctttc gattttgccg aggcgcagcc gttctatgtg
     5461 aactcgccct tcggaatcat gcttgggcat aacggcaact tgaccaattc cgattcgctg
     5521 cgtgaagaga tgttccggca ggaccgccgg cacatcaaca ccaactcgga ttccgaagtg
     5581 ctgcttaacg tgctggctca tgaactgcag gaccgtgcgc gcggaacgca gctcgatgtc
     5641 gacaccatct ttgccgcagt gcagggcgtt caccgccgct gccgtggcgc ctacgcggtg
     5701 gttgcgatga tcgccggcta cggcctgctg gcttttcgcg acccgcacgg catccggccg
     5761 atggtctttg gccgcaacga tgccgccgat ggcaccgagt atctggtctc ctccgagtcg
     5821 gtggcgctcg ataccctggg tttcgaactc gaacgcgatg ttgcgcctgg cgaagcgatc
     5881 ttcatcgacc tcgaccaccg cctgcattcg cgccagtgcg ccgaccgccc ggtgctctcg
     5941 ccgtgcatct tcgagtacgt ctacctcgct cgcccagatt cggtgatgga tggcgtttcg
     6001 gtttacgaga cgcgcctgcg catgggcgag cacctcgccg aaaaggttcg ccgcgtcctg
     6061 ggtccggaac agatcgacgt ggtcattccg attcccgatt cgagccgtcc ggcggcgatg
     6121 cagctggcgc aggccctggg gctcagctat cgcgaaggct tcgtcaagaa tcgctacatc
     6181 ggtcgcacct tcatcatgcc cgggcagtcg acgcggcgga agaacgtgcg gcagaagctc
     6241 aatactgtcg atcgcgaatt cgagggcaag cgcgtgctgc tcgttgacga ctcgatcgtc
     6301 cgcggcacga ctagccgcga gatcgtgcag atggcgcgcg atgccggagc gacgcgggtc
     6361 tatttcgcct ccgccgcacc gccggttcgt tatccgaacg tctacggcat cgacatgccg
     6421 acgctggcgg agctgatcgc gacgggccgc accgacgcac agatcgccgc cgaaatcggc
     6481 gccgacgcgc tggtctatca ggatctcgat gcgcttaagg cggccgttcg cggctccaat
     6541 cccgccttgt cgggatttga cgcctcgtgc ttcgacggcc gttacatcac cggcgacgtc
     6601 agtgccgagt accttgccga gatcgaactg cgccgcggcg gcgcttccca ctcggcggcg
     6661 agcgacggga attcaagccg gcagctgatg cttagcctcc cggacgagga cgagctggga
     6721 gaggcggctt gagcaagggc ttgccggaag ggctgcatct cgattcgctg gcggttcgtg
     6781 ccgggcagga acgcagccag ttcaacgaac attccgaagc cctgtacctg acctcgagct
     6841 ttgtcttcga cagcgcggca caggcggccg cccgtttttc gggcgacgag gacggcaacg
     6901 tctattcgcg tttcaccaat ccgaccgtga ccatgttcga ggagcggctg gccgcgctcg
     6961 aaggcgccga ggattgcgtg gcaacggcct cgggcatgtc ggcgatcctc tcgctggtca
     7021 tggcacatct caaggctggc gaccacatcg tcgcctcggg cagcctgttc ggcgcgactg
     7081 tgcagatgtt taccaacctc ctgccacgct tcggcatcga gaccagcttc gtcgcgcagt
     7141 ccaggccaga cgactggcag gcggcgctgc gcccgaatac gcgtttgctc tttgtcgaga
     7201 cgccgtcgaa tccgctgacc gagattgccg acatcggcgc actggtggcg attgcccgcg
     7261 cccacggggc gctgctcgcc gtcgacaact gcttctgtac gccggctttg cagcgtccgc
     7321 tcgatctggg agcggatctc gtcatccatt cggcgaccaa gtacctcgac gggcaggggc
     7381 gggtgctggg cggcgccgtc tgcggccgca aggcgctggt ggaagaggtg ttcaagtttc
     7441 tgcgcacggc cggaccgacg ctctcagcat tcaacgcctg ggtgctgctc aagggcatcg
     7501 agacactcgg cctgcgcatg gctgcgcaat cgcgggcggc gcttgaactg gcgcgctggc
     7561 tcgaagcgca tccgaaagtc ggtcgtgtct actacccggg tctggacagc catccgcagc
     7621 acgcgctggc gagccggcag cagaagtcgg gcggcgcgat tctcgggttt gaagtgcagg
     7681 gagggagaag cgaagcctgg cgcgtcgtcg atcattgccg tctgctgtcg atcaccgcca
     7741 atcttggcga cgtcaagacc acgcttacgc acccggcaac gacgacgcac ggccgcatca
     7801 gtgccgaggc gcgcaccgcc gctgggatat cggagagcct cttgcgtgtt gcagttggcc
     7861 tggaggccct gtc
//
LOCUS       DATDAA010000323         2990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_443585,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000323 DATDAA010000000
VERSION     DATDAA010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443585"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VIS73_09925"
     CDS             <1..572
                     /locus_tag="VIS73_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5763510.1"
                     /translation="FEARLPDLVVIDINLKDEVEGGFELCRELRSRSPDLPIIFLTAR
                     DSEFDAVSGLRLGADDYLTKDISLPHLMARVAALFRRLDAIRQPSGKESRIRRGELEM
                     DIDRMTVRWRDRPVGLTLTEFWMVHALARYPGHVKNRQQLMDAAQAVLDDNTITSHIK
                     RIRRKFLAIDPDFAAVETVYGMGYRWLTE"
     gene            569..2605
                     /locus_tag="VIS73_09930"
     CDS             569..2605
                     /locus_tag="VIS73_09930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5763511.1"
                     /translation="MNLRRQLLLVSLLTLVLPWGGCQFIRETESALREGQQQMLAGTA
                     QAIADSLSQFPYEFITEGGGGRLYLHPLQHAPLLDGYADDWTIAAGALENLRGKDGII
                     RYVAGAWQQHLYLYVDARDARKVYRPATGTARPRRYDHVALTSVGGDGRQTVFRFAAE
                     APGEIVASREADGRVERDTRVTAYWLDTAAGYQLEIRLPRNLAGARLGITVNDTDDAS
                     TAGVESASFGGGPPGPLAGVSPVLQSVAAGYVQPGLRLIITDKDGWRLAQAGSVSSSG
                     GPQAAPSSGWMRIAYDLLLEPGAEAALAEPSPNGREQQGYISGALGGQAASRWFRSPD
                     TGRAVVSVAQPIWSGTVQTGAIVLQQGTDAILSLTNQALTRLIVLTLAVTVVVAAVLI
                     GYASWLSLRIRALSSAALEALDEKRLKSALPSALAGDEVGDLSRSFSSVLRQLGIYND
                     YLQSLASKLSHELRTPLTIVRSSLENLEHEKLSAEAIEYTERAREGTERLQKILNAMS
                     EASRTEELIDNVEPEHFDLARILEPTVAAYADAWPERRFRFENRADAGGVNGAPELII
                     QMLDKLVDNAVDFSNSGDTIVVSLEREAETVVLAVTNPGPPLPEELREGLFRSMVSFR
                     KNDAGKHLGLGLYIARLIAEGHEGSIDAVNTGEGVSFRVRLPLSAPRRPVQKPR"
     gene            complement(2590..>2990)
                     /locus_tag="VIS73_09935"
     CDS             complement(2590..>2990)
                     /locus_tag="VIS73_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5763512.1"
                     /translation="SVRFLFNRQPVEIIGADHVTGVKLVETELGEPGPDGRRHPSPVA
                     GSEHILPADAVIIAFGFRPNPPPWFADFGIATLPNGRVRVDSIGPYPFQTTNPRIFAG
                     GDMVRGSDLVVTAVFEGREAARGITRYLGF"
BASE COUNT          501 a         1008 c         1003 g          478 t
ORIGIN      
        1 ccttcgaggc cagacttccg gacctggttg tcatcgatat caatctgaag gacgaagtgg
       61 aaggcggctt cgagctctgc cgggaactcc gcagccgttc gcccgatctg ccgatcatct
      121 tcctgacggc gcgggacagc gagttcgacg cggtatccgg actgcggctc ggcgccgacg
      181 actacctcac caaggacatc agcctgccgc acctgatggc ccgggtcgcc gccctgttcc
      241 gtcggctcga cgccattcgg caaccgtcgg gcaaagaaag ccggatccgg cgaggcgagc
      301 tcgaaatgga catcgaccgg atgaccgttc gctggagaga ccggccggtc gggctcacgc
      361 tgaccgagtt ctggatggtc cacgcgctcg cgcgctaccc cgggcacgtg aagaatcgcc
      421 agcagttgat ggatgcggcc caggccgtgc tggacgacaa cacgatcacc tcccatatca
      481 agcgaatccg gcgcaagttc ctcgccatcg acccggattt cgccgccgtc gagaccgtct
      541 atggcatggg ctatcgctgg ctgactgaat gaacctgcgc cggcagttgc tcctggtcag
      601 cctgttgacg ctggtgctgc cctggggggg atgccagttc atccgcgaga cggagtccgc
      661 cctgcgcgag ggacagcagc agatgctggc cggcacggct caggcgattg ccgactcgct
      721 gtcgcagttt ccctacgaat tcatcacgga aggcggcggc ggccggctct acctgcaccc
      781 attgcaacac gcgccgctgc tcgacggtta tgccgacgac tggaccatcg ccgccggcgc
      841 gctcgaaaac ctgcgcggca aagacggcat cattcgctac gtcgccggcg cctggcagca
      901 gcacctgtac ctgtacgtcg acgccaggga cgcgcgcaag gtgtatcgtc cggcgacggg
      961 cacggcccgg ccacggcgtt acgaccacgt tgcgctgacg agcgtcggcg gggacgggcg
     1021 ccagacggtc ttccgctttg ccgctgaggc gccgggagag atcgttgctt cacgcgaagc
     1081 ggacggccgg gtcgagcgcg acacccgggt tacggcctac tggctggaca ccgccgccgg
     1141 gtaccagctc gagatccgcc tgccgcgtaa cctcgccggc gcccggctcg ggatcacggt
     1201 gaacgatacc gacgatgcgt ccacggccgg cgtcgagagc gcgagcttcg gcggcggccc
     1261 gcccgggcct cttgccggcg tgtcgccggt tctgcagagc gtcgccgcgg gctacgtgca
     1321 gccggggctg aggctgatca ttaccgacaa ggacggctgg cgcctggcgc aggccggcag
     1381 cgtgtcctct tccggcgggc cgcaggcggc gccgtcgtcc ggctggatgc gcatcgctta
     1441 cgacctgctt ttggagccgg gcgccgaagc cgcgctcgcc gagccgtcgc cgaacggcag
     1501 agagcagcag ggatatattt ccggggcgct cggcggccag gccgcgtcgc gctggttcag
     1561 gagccccgat accggccggg cggtggtctc ggttgcccag cctatctggt cgggcacggt
     1621 ccagaccggc gccatcgtgc tgcagcaggg gaccgatgcc atcctgagcc tgaccaacca
     1681 ggcgctgacc cggctcatag tgctgacgct cgccgtaacc gtcgtggtcg ccgcggtcct
     1741 gatcggctac gcgagctggc tgtcgctgcg catccgcgca ctgagctcgg ctgcgctcga
     1801 ggcactcgac gaaaaacgcc tgaagagcgc gttgccgagc gcgctcgccg gcgacgaggt
     1861 cggcgatctg tcgcgcagtt tctccagcgt gctgcggcag ctgggcatct acaacgatta
     1921 cctgcagtcg ctcgccagca agctctctca cgagctgcgc acccccctta cgatcgttcg
     1981 ttcgtcgctc gagaacctgg aacacgagaa gctgtcggcc gaggcaatcg agtacaccga
     2041 gcgggcgcgc gagggcaccg aacgcctgca gaagatcctg aacgcaatga gcgaggccag
     2101 ccgcaccgag gaactgatcg acaacgtcga gccggaacat ttcgatctcg cccgaatcct
     2161 cgagccgacc gttgccgctt acgcggacgc ctggccggaa cggcgctttc gattcgaaaa
     2221 cagggcggac gccggcggcg tcaacggcgc gcccgagctc atcatccaga tgctcgacaa
     2281 gctggtcgac aacgcggtcg atttcagcaa ctccggcgac acgatcgtcg tttccctgga
     2341 gcgcgaggcc gaaaccgtcg tgctcgccgt gaccaatccg ggtccgccgt tgcccgagga
     2401 gctgcgggaa ggccttttca gatcgatggt ctcgtttcgc aagaacgacg cgggcaaaca
     2461 cctgggactc ggcctctata tcgcccggct catcgccgag gggcacgagg gctccataga
     2521 cgccgtgaac accggcgagg gcgtttcttt ccgcgtgcgg ctgccgctgt cggcgccgcg
     2581 ccggcccgtt cagaaaccga gatagcgggt gatgccgcgg gccgcctcgc ggccttcgaa
     2641 caccgccgtg acgaccaggt ccgagccgcg aaccatgtcg ccgccggcga agatcctggg
     2701 attggtcgtc tggaaagggt atgggccgat cgaatcgacg cgcaccctgc cgttcggcag
     2761 cgttgcaatg ccgaagtcag caaaccaggg cggcggattc gggcgaaacc cgaaggcgat
     2821 gatgaccgcg tcggcgggga gtatgtgctc gctcccggcc acgggactcg ggtggcgcct
     2881 gccgtccggc ccgggctcgc cgagttccgt ttcgacgagc ttcacgccgg tgacgtgatc
     2941 ggcgccgatg atctctaccg gctgccggtt gaaaaggaat cgaacgctct
//
LOCUS       DATDAA010000324         6472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_268189,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000324 DATDAA010000000
VERSION     DATDAA010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268189"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VIS73_09940"
     CDS             complement(<1..1194)
                     /locus_tag="VIS73_09940"
                     /inference="COORDINATES: protein motif:HMM:NF002204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEY5763513.1"
                     /translation="MNAGRQAFLISAAHKSSGKTTVSIGLAAALNSRGLVVQTFKKGP
                     DYIDPMWLTQATGRACRNLDLYLSDEAACRRAFADYGRDAAVAIVEGNKGLYDGLALD
                     GSNSNAALAHALDLSVILVIDCRGMIRGVAPLVLGYQAFDPGLRIAGVILNRVGGPRH
                     ERKLREVLACYTQVPVLGAIPETPELSLTERHLGLMPSNEDPEAADKIATLARITARC
                     VDIDALLALCPAAPSAPPATTPVIPFPARAPRANRDCTLRVGVIRDRAFGFYYADDLD
                     ALRAAGGELHFIDSLRDNRLPEIDALFIGGGFPETLAASLEANAPLRGELRRAIDAGL
                     PTYAECGGLMYLSRGIQWQGHRYEMVGVIAAETVVRPKPVGRGYVELEATAEHPWWPA
                     GTRLKG"
     gene            complement(1226..2461)
                     /gene="nrfD"
                     /locus_tag="VIS73_09945"
     CDS             complement(1226..2461)
                     /gene="nrfD"
                     /locus_tag="VIS73_09945"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY5763514.1"
                     /translation="MSAKPFAQLEERYFYALVGAGLLVLLVALLAFHDTETRGHIITG
                     MNNQIVWGTPHVFAIFLIVAASGVLNIASMGSVFGKAIYKARAPLSGLLSIAMLAGGL
                     MVLLLDLGRPDRLIVAITHYNFKSIFAWNIILYTGLFAIVAVYLWTMMERRMNRYSKP
                     AGFTAFFWRIILTTGTGSIFAFLVARQGFGTAVLAPLFVVMSFSWGMAVFYIVQAAMF
                     SWNDIELHGHVQKRMNNLLGIFIAGTFYFVVVYHLTNLYFERQIDFERFILLEGGAPT
                     LALWLGYVLIGTALPLLLLYHPTLGRPRDTLFASIFVLVGAFALLYSLIIGGQSFPLE
                     LFPGFEASSSFGDGQIAAYRPSLPEILLGLGGLAAAFLITTIGVRILPFLPDVSEHDD
                     RHAPAAGSAAAAAPATAAK"
     gene            complement(2465..3229)
                     /locus_tag="VIS73_09950"
     CDS             complement(2465..3229)
                     /locus_tag="VIS73_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY5763515.1"
                     /translation="MSSDRKPRAAEAAPNGKRRAFVVAGATAMLGTLIAPGIRLIEVA
                     GASEAAGKTRLASDKVRWGLLIDANRCASDCDVCVQACNKENHLPGGTKPTDVQWIRK
                     IDLKDKGSGRELSLPMMCQHCANPPCVDVCPTGASMKRADGIVLVDRHSCIGCRYCMM
                     ACPYKARSFVHEPVTDQNPLVPRGKGCVESCTFCVHRVDAGRLPACVEACPNQAMLFG
                     DLNDADSAISRRIRSVASTRVRADLGLDIAVRYEGI"
     gene            complement(3226..3618)
                     /locus_tag="VIS73_09955"
     CDS             complement(3226..3618)
                     /locus_tag="VIS73_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763516.1"
                     /translation="MKRLHNAIIVLFSAASLLAAGPALAEVPKPSVEIAQPDTQCVAP
                     PEEMRRNHMAMLTHQRDRTLRLGERGAPITLNGCINCHASKASGSVAGAKGDFCAGCH
                     AYAAVKLDCFECHQPKASLASLSKEMPR"
     gene            complement(3625..5598)
                     /locus_tag="VIS73_09960"
     CDS             complement(3625..5598)
                     /locus_tag="VIS73_09960"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY5763517.1"
                     /translation="MSMSTQKETTALNFRRYKEGDVDYDDYHEKIFNASWTHKCPTYI
                     QSTPPCQGSCPSGEDIRGYLNIVRGIEKPPLGADGKPVMPWQEYAWRRLTQANPLPAV
                     MGRVCPAPCETGCNRNEVEDHVGINSVEHFLGDYAIQNKLQYIKPEQSTGKKIAIVGS
                     GPAGLSCAYQLALKGHEVTIFDDHEHLGGMMRYGIPGFRTPRDLLDAEIQRILDLGVK
                     TRMKCRVGTDITLEQINKDFDAVFLGLGAQAGRVLPVEGAQNVPNVITATSFLKAFND
                     GRLQHVGKRVVVVGGGDTSIDVATVARRLGHIEHAKPTDFDDAIAGRMAHDVAEVSAK
                     QGAEVTLTSVFPIDKMQANKHEIEQALAEGIAIKGGLVPVGLVKDANGRATALRVAEC
                     DAKMVGGKLDMKIKEGSEFDIETDLVVSAIGQGVDFTGLEVFDNGKGGVSADRNYQVA
                     GKPGVFAGGDVLRPHLLTTAVGHGAIAAEGIDRFLLGEEQEKRPKIDVHVFDLMRKLV
                     EKGQQIGEIKEPLRGTDGARDAVHNYDNRSDRYVITHKELFLGHFPYTPRNVRSVITL
                     DKESALGNFDERLQALVEEKAVAEAKRCMSCGLCFECDNCVVYCPQTAVKKVPKKEST
                     TGRYVYTDYDRCIGCHICRDVCPTGYIQMGLGE"
     gene            complement(5642..>6472)
                     /locus_tag="VIS73_09965"
     CDS             complement(5642..>6472)
                     /locus_tag="VIS73_09965"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY5763518.1"
                     /translation="LITPSADFFAEPHIDSLIGYGKVFHAAGLSWTLSSKASEAGNFG
                     LFIGNYDQLRTIALRIREAALELGVKRIVVGECGHAWRVAYSFWNTLTGIGDGGDDPF
                     AKMLQKQLDHRYKQPAHICEVTWDLIQQGALSFDKDANDHRIVTFHDSCNVARGSRMG
                     DTAGGQFEIPRNIVRAVANHFHEMSPDTTLERTFCCGGGGGLLTDELMDIRVKGALPR
                     MEALQRVVDEHGVNFMATICAICKAQFTKVLPYYGFKMSLVGGVHQLVSTAIRLGDKP
                     "
BASE COUNT         1066 a         1929 c         2310 g         1167 t
ORIGIN      
        1 gcccttgagg cgggtgccgg cgggccacca ggggtgctcg gcggtcgctt cgagttcgac
       61 gtagccgcgc ccgaccggtt tgggccggac aacggtttcg gcggcgatta cgccgaccat
      121 ctcgtagcga tgtccctgcc actggatgcc gcgcgagagg tacatcaggc cgccgcactc
      181 ggcgtaggtc ggcaggccgg catcgattgc gcggcgcagc tcgccgcgca acggtgcgtt
      241 ggcttcgagg ctggcggcaa gcgtctcggg aaagccacca ccgatgaaca gcgcgtcgat
      301 ttcgggcagg cggttgtcgc gcagcgaatc gatgaagtgc agctcgccgc cggcggcgcg
      361 caacgcatcg aggtcgtcgg catagtagaa gccgaaagca cggtcgcgga ttacgccgac
      421 gcgcagcgtg cagtcacggt tggcgcgtgg cgcgcgggcc ggaaacggaa tgaccggcgt
      481 cgtcgcgggc ggagctgatg gcgctgcagg gcacagcgct agcagcgcgt cgatgtcgac
      541 gcagcgcgcg gtgatccgcg ccagcgtggc tatcttgtcg gcggcctcgg ggtcttcgtt
      601 gctcggcatg agaccgaggt ggcgctcggt cagcgacaat tcaggggttt cgggaatggc
      661 gccgagcacg ggaacctggg tgtagcaggc gagcacttcg cgtagcttcc gctcgtggcg
      721 cgggccgccg acgcgattga ggatgacgcc ggcgatgcgc aagccggggt cgaaggcctg
      781 atagccgagc accagcggtg ccacgccgcg gatcatgccg cggcagtcga tgaccaggat
      841 caccgacagg tcgagcgcgt gggcgagcgc cgcgttgctg ttgctgccgt cgagcgcgag
      901 tccgtcgtac aggcccttgt tgccctcgac gatcgccacc gcggcgtcgc ggccgtagtc
      961 ggcaaaggct ctccggcagg cggcttcgtc ggagagatag agatcgaggt tgcggcaggc
     1021 gcggccggtg gcctgcgtga gccacatcgg gtcgatgtag tcgggtccct tcttgaatgt
     1081 ctgcaccacc agtccgcgcg aattcagcgc cgccgcgagg ccgatgctga ccgtggtttt
     1141 tcccgacgac ttgtgggcgg ccgagatgag gaaggcttgg cggccggcgt tcatcgctca
     1201 gccctgacga ccgtggtggc tcacgttact tggcggccgt ggccggtgcg gcggcggctg
     1261 cagagcccgc tgcgggcgcg tgtcggtcgt cgtgctcgga gacatcgggg aggaagggca
     1321 ggatgcgaac gccgatcgtg gtgatcagga aggcggcggc gaggccgccg agcccgagca
     1381 ggatttcggg caggctcggg cggtaggcgg cgatctggcc gtcgccgaaa ctgctgctcg
     1441 cttcgaaacc ggggaagagt tcgagcggaa aggactggcc gccgatgatc agcgaataga
     1501 gcagggcaaa ggcgcctacc agcacgaaga tcgaggcgaa cagcgtgtcg cgcgggcggc
     1561 caagggtcgg gtggtagagc agcagcagcg gcagcgcggt tccgatcaat acgtagccga
     1621 gccacagcgc gagcgtcggt gcgccgcctt cgagcaggat gaagcgctcg aaatcgatct
     1681 gtcgttcgaa gtagagattg gtcaggtggt agacgacgac gaaatagaag gtgcccgcga
     1741 tgaagatacc gagcagattg ttcatgcgct tctgcacgtg gccgtgcagc tcgatgtcgt
     1801 tccagctgaa catcgcggcc tgcacgatgt agaagaccgc catgccccag ctaaaggaca
     1861 taacgacgaa cagcggcgcg agtacggcgg tgccgaatcc ctggcgcgcg acgaggaagg
     1921 cgaagatcga gccggtacca gtcgtgagga tgatccgcca gaaaaacgcg gtgaagccgg
     1981 cgggctttga gtaacggttc atgcgccttt ccatcatcgt ccacagatag acggcgacga
     2041 tcgcgaagag accggtgtat aggatgatgt tccaggcgaa aatcgacttg aagttgtagt
     2101 gggtgatcgc gacgatcagc cggtcggggc gaccgaggtc gagcagcaga accatcaggc
     2161 cgccggcgag catggcgatc gagagcaggc cggagagtgg cgcgcgcgcc ttgtagatgg
     2221 ccttgccaaa caccgaaccc attgaggcga tgttgagcac gcccgaggcg gcgacgatca
     2281 ggaagatcgc gaagacgtgc ggcgtacccc agacgatctg gttgttcatg ccggtgatga
     2341 tgtggccgcg ggtttcggtg tcgtggaagg cgagcagcgc caccagtagg accagcaggc
     2401 cggcgccgac cagcgcgtag aagtagcgtt cctcgagttg ggcgaagggc ttggcggaca
     2461 tggctcagat tccctcgtag cggaccgcga tgtcgaggcc gaggtcggcg cgcacgcggg
     2521 tcgaggcgac gctgcgaatg cgtcgtgaga tggcgctgtc ggcatcgttg aggtcgccga
     2581 aaagcatcgc ctgattgggg caggcttcga cgcaggcggg cagccggccg gcatcgacgc
     2641 ggtggacgca gaaggtgcag gactcgacgc agcccttgcc gcgaggaacc agcgggttct
     2701 ggtcggtgac cggttcgtgg acgaagctgc gcgccttgta ggggcaggcc atcatgcagt
     2761 agcggcagcc gatgcagctg tggcggtcga ccagcacgat gccgtcagcg cgtttcatcg
     2821 aggcgccggt cgggcagacg tcgacgcagg gcgggttggc gcagtgctgg cacatcattg
     2881 gcagggagag ctcgcggccg ctgcccttgt ccttgagatc gatcttgcgg atccattgca
     2941 cgtcggtcgg cttggtaccg cccggcaggt ggttttcctt gttgcacgcc tgcacgcaga
     3001 catcgcagtc gctggcgcag cggttggcgt cgatcagcag gccccagcgc accttgtccg
     3061 aagccagccg ggtcttgccc gccgcctcgc tggcgccggc gacttcgatc agccggattc
     3121 ccggcgcgat tagcgtgccg agcattgccg tcgcgccggc gacaacaaag gcccggcgct
     3181 tgccgttcgg cgcggcctcg gcggctcgcg gcttgcggtc tgaactcatc gcggcatttc
     3241 cttcgacagg ctggcgagcg aggccttggg ttggtggcac tcgaagcagt cgagcttgac
     3301 ggcggcgtag gcatggcaac ccgcgcagaa gtcgcctttg gcgccagcca ccgaaccact
     3361 ggccttgctc gcgtggcaat tgatgcagcc gttcagcgta atcggcgcgc cgcgctcgcc
     3421 aagccgcagc gtgcggtcgc gctggtgggt gagcatcgcc atgtggttac ggcgcatttc
     3481 ttcaggcgga gcgacgcatt gcgtgtcggg ctgcgcgatc tcgaccgagg gcttcggaac
     3541 ctcggcaagg gcgggcccgg ccgccagcag gctggcagcc gagaacagca cgatgatcgc
     3601 gttgtgcagc cgtttcatcg tggcctactc gccgagtccc atctggatgt agccggtcgg
     3661 gcagacgtcg cggcagatat ggcagccgat gcagcggtcg tagtcggtgt acacgtagcg
     3721 gccggtggtc gactccttct tcggcacctt cttgaccgcg gtctgcgggc agtagacgac
     3781 gcagttgtcg cattcgaagc agaggccgca gctcatgcag cgcttggctt cggcgacggc
     3841 cttttcctcg accagggcct gcaggcgctc gtcgaagttg ccgagggcgc tttccttgtc
     3901 gagcgtgatc accgagcgga cgttgcgtgg cgtgtaggga aagtgtccga ggaacagttc
     3961 cttgtgggtg atgacgtagc gatccgagcg gttgtcgtag ttgtgcacgg cgtcgcgggc
     4021 gccgtcggtg ccgcgcagcg gctccttgat ctcacctatc tgctgcccct tctcgaccag
     4081 cttgcgcatc aggtcgaaga cgtggacgtc gatcttcggc cgcttctcct gttcctcgcc
     4141 caagaggaag cggtcgatgc cttcggcggc aatcgcgccg tggccgactg ccgtggtcag
     4201 caggtgcgga cgcagtacgt cgccaccggc gaagacgccg ggcttgccgg cgacctgata
     4261 gttgcggtcg gcgctgacgc cgcccttgcc gttgtcgaag acttccaggc ccgtgaagtc
     4321 gacgccctgg ccgatcgccg agacgacgag gtcggtctcg atgtcgaact cgctgccttc
     4381 cttgatcttc atgtcaagct tgccgccgac catcttggcg tcgcactcgg cgacgcgcag
     4441 cgcggtggcg cggccattgg catccttgac caggccgacc ggaacgaggc cacccttgat
     4501 cgcgatgcct tcggcgagtg cctgttcgat ctcgtgcttg ttcgcctgca tcttgtcgat
     4561 cgggaacacc gaagtcagcg ttacttcggc accttgcttg gccgatacct cggcgacgtc
     4621 atgggccatg cggccggcaa ttgcgtcgtc gaaatcggtc ggcttggcgt gctcgatgtg
     4681 gccgagacga cgggcaacgg tcgccacgtc gatcgaggta tcgccaccgc cgaccaccac
     4741 gacacgcttg ccaacgtgtt gcaggcgccc gtcgttgaag gccttgagga aggaggtggc
     4801 ggtgatgacg ttggggacgt tctgcgcacc ttcgaccggc agcacccgcc cggcctgcgc
     4861 accgaggccg aggaatacgg cgtcgaagtc cttgttgatc tgttcgagcg tgatgtcggt
     4921 gccgacgcgg cacttcatgc gcgtcttcac gccgaggtcg aggatgcgct ggatctcggc
     4981 atcaagcagg tcgcgcggcg tgcggaagcc tgggatgccg tagcgcatca tgccgccgag
     5041 gtgctcgtgg tcgtcgaaga tcgtcacttc gtggcccttg agcgcgagct ggtaggcgca
     5101 ggagaggccg gccgggcctg aaccgacgat ggcgatcttc ttgccggtcg attgctcggg
     5161 cttgatgtat tgcagcttgt tctggatcgc gtagtcgccg aggaagtgct cgaccgagtt
     5221 gatgccaacg tggtcctcga cctcgttgcg gttgcagccg gtctcgcagg gcgccgggca
     5281 gacgcggccc atcacggccg gcagcgggtt ggcctgcgtc agtcggcgcc aagcgtattc
     5341 ctgccagggc atgaccggct tgccgtcggc gccaagcggc ggcttctcga tgccacggac
     5401 gatattgaga tagccgcgaa tgtcctcacc tgaggggcag ctgccctgac acggcggcgt
     5461 gctctggatg taggtcgggc acttgtgggt ccaactcgcg ttgaagattt tctcgtgata
     5521 gtcgtcgtaa tcgacatcgc cttccttgta gcggcggaag ttcagcgcgg tggtttcctt
     5581 ctgggtgctc atcgacatgt gcgtctccgt ataggtgtta tgggccgttg cgtcgtgccg
     5641 atcagggctt gtcgccgagg cgaatcgccg tgctgaccag ctggtggacg ccgccgacca
     5701 agctcatctt gaaaccgtag taggggagga ccttggtgaa ctgcgccttg cagatggcgc
     5761 agattgtcgc catgaaattc acgccgtgct cgtcaaccac gcgctgcagc gcctccatgc
     5821 gtggcagcgc gcccttgacg cggatgtcca tcagttcgtc ggtcagcagt ccgccgccgc
     5881 cgccgcagca gaaggttcgc tcaagcgtgg tgtcgggcga catctcgtgg aagtggttgg
     5941 cgaccgccct gacgatgttg cgcggaatct cgaactgtcc gcccgccgta tcgcccatgc
     6001 gtgagccgcg ggcgacgttg cacgagtcgt ggaaggtgac gatgcggtgg tcgttggcgt
     6061 ccttgtcgaa ggaaagcgcg ccctgctgga tcagatccca ggtcacctcg cagatatgcg
     6121 cgggctgctt gtagcggtgg tcaagctgct tctgcagcat tttggcgaag gggtcgtcgc
     6181 cgccatcacc gatgccggtc agcgtgttcc agaagctgta ggcgacgcgc caggcgtggc
     6241 cgcactcgcc gacgacgatg cgcttgacgc cgagttcgag cgccgcttcg cggatgcgta
     6301 gtgcgatggt tcgcagttgg tcatagttgc cgatgaacag cccgaagtta ccggcctcgg
     6361 acgccttcga cgacagcgtc caggagaggc cggccgcgtg gaagaccttg ccgtagccaa
     6421 tcaggctgtc gatgtgcggc tcggcgaaaa aatcggccga aggcgtgatc ag
//
LOCUS       DATDAA010000325         2666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_338760,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000325 DATDAA010000000
VERSION     DATDAA010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338760"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..993
                     /locus_tag="VIS73_09970"
     CDS             307..993
                     /locus_tag="VIS73_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY5763519.1"
                     /translation="MDTFGIVLRFFQNGGIFMYIILGVLVLGLAIALERWLFLTSAKI
                     SNRRLWDRIFPMMQGADFRRAQKTASESSTSIGNMIGFGLGCMFTAQGTADRRMLPAV
                     EMAMEESLMEAVPRLEKRTPYLAILANVATLLGLLGTIIGLIDAFSAVANADPAEKGN
                     LLSASISVAMNTTAFGLIAAIPLLLVHALLQNKTTEIVDSLEMAAIKVLNLIRQPETA
                     VSGDAVPMKR"
     gene            1007..1525
                     /locus_tag="VIS73_09975"
     CDS             1007..1525
                     /locus_tag="VIS73_09975"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY5763520.1"
                     /translation="MASGLRRRRSHPPEELNVTAFMNLMVVLVPFLLISAVFNQITIH
                     ELNLPTLSEPAVEPKEDKPTLVLEVIIRKDSLDVQDRNSGRLKLIPNGAIGHDFVALK
                     EKLREIKVAFPAVAAATLLLEQDVPYDVLIQTMDAVRTDVVKKDGADAPVELFPDVSI
                     GDAPRLAAEATP"
     gene            1522..2025
                     /locus_tag="VIS73_09980"
     CDS             1522..2025
                     /locus_tag="VIS73_09980"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY5763521.1"
                     /translation="MKQTSRRAKRMNKQHKRRRGGAALNLVALMDIFTILVFFLLVNT
                     SGIQPQGTKVELPQSIAEKVPAETILITVSNEAIVVQGRRVAETPAVLATDSLIIEEL
                     RQELSYHAKRTGTGDETEERPVTIMGDKEIPYRLLKRIMLTCTQTGYSQVSLAVTRKG
                     KAGGAAS"
     gene            2022..>2666
                     /locus_tag="VIS73_09985"
     CDS             2022..>2666
                     /locus_tag="VIS73_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763522.1"
                     /translation="MSTAVVAAFTPALPWAVNPVEQQRGRRIAATAVVLFVLFSALMT
                     VFDPPPIDRAEAEKLPPRLAKLVLEKKEAPPPKPEPVIEKPKEEPKPEVKEETPNEEP
                     KVKPTEQQVEKAREKAKKSGLLAMKDQLAALRDLSTASSLRQPQAKVGAEGTEQRVER
                     DLIGRKATSGSGGVATTAVAHSGGGTLAGRTTTQVEVPVSAGESLAETRAKARSP"
BASE COUNT          516 a          857 c          843 g          450 t
ORIGIN      
        1 cgaacaagtc cgaagcggct gcaccaactg ctcagacaag acccgcgccg agcgtttctc
       61 gaaccgctgc gagtactttg ccggccgtgc cgatcgtgct tgatagcgac ttgccaatcg
      121 tcgacgatcc gcttgcacgg ccgcttcccg tcaggtagtc gtacgcgtgc gatcgagcgg
      181 tcctggtagt caatttgtag gccgcggcat gcctcggtac ccgaaatgca tgcggcgagg
      241 tggttctctg ccgccgaatc gtcgataatc tcgccccatt cgcgaaccaa tctgaggagt
      301 caacgcgtgg ataccttcgg gatcgttctc cgcttttttc aaaacggcgg catcttcatg
      361 tacatcatcc tcggcgtcct tgtgctcggt ctggccatcg cgctcgagcg ctggctgttc
      421 ctgacgagtg ccaagatctc caatcgccgt ctctgggatc gcatctttcc gatgatgcag
      481 ggcgccgatt tccgcagggc gcaaaagacc gccagcgaat cgtcgacatc gatcggtaac
      541 atgatcggtt tcggtctcgg ctgcatgttt accgcgcagg gaaccgctga tcggcggatg
      601 ctgccggccg tcgagatggc gatggaagag agcctgatgg aagcggtgcc gcggctggaa
      661 aagcgcacgc cctatctggc gattctcgcc aacgtcgcga cgctgctcgg cttgcttggc
      721 accatcatcg gcctgatcga cgctttcagc gccgtcgcca acgccgatcc ggcggaaaag
      781 ggcaatctgc tgtcagccag tatctcggtg gcgatgaaca ccactgcgtt tggcctgatt
      841 gcggcgattc cgctgctgct cgtgcatgca ctgctgcaga acaagaccac cgaaatcgtc
      901 gacagcctcg aaatggcggc gatcaaggtg ctcaacctga tccgtcagcc tgagactgcg
      961 gttagcggcg atgccgtgcc aatgaagcgc tgaatcggaa tcgaccgtgg ccagcggcct
     1021 gcgacgccgg cggagccatc cgcccgagga actcaacgtc accgccttca tgaatctgat
     1081 ggtggtgctg gtgccgttcc tgctgatctc ggcggtcttc aaccagatca ccatccatga
     1141 gctgaacctg ccgacgctgt ccgagccggc ggtcgagccc aaggaggaca agccgacgct
     1201 cgtgctcgaa gtgatcatcc gcaaggacag cctcgacgtg caggatcgca acagcggccg
     1261 cctcaagctg attcccaacg gcgcgatcgg ccacgacttc gttgccctca aggagaagct
     1321 gcgcgaaatc aaggtggcgt ttcccgccgt tgccgccgcg acgcttctgc tcgagcagga
     1381 tgttccctac gacgtcttga tccagaccat ggacgccgtg cggaccgacg tcgtcaagaa
     1441 agatggcgcc gatgcgccgg tcgaactgtt cccggacgtt tccatcggcg atgcaccgcg
     1501 acttgccgcg gaggccaccc catgaagcag acgtcgcgcc gcgcaaagcg catgaacaag
     1561 cagcacaagc gtcggcgcgg tggtgctgcg cttaacctcg tggcgctgat ggacatcttc
     1621 acgattctgg tgttcttcct gctggtcaac acctcgggca tccagccgca ggggaccaag
     1681 gtcgaactgc cgcaatcgat tgccgagaag gtgcccgccg agaccattct catcaccgta
     1741 agcaacgagg cgatcgtcgt ccaggggcgg cgcgtggctg agacgcccgc cgtgctggcg
     1801 accgactcgc tgatcatcga ggagctgcgg caggagctca gctaccacgc caagcgcacg
     1861 ggtactggcg acgagaccga ggaacgcccg gtgacgatca tgggcgacaa ggaaattccc
     1921 taccggctgc tcaagcgcat catgctgacc tgcacgcaga ccggctacag ccaggtgtcg
     1981 ctggcagtca ctcggaaggg caaggccggg ggcgctgcct cgtgagcacg gcagtcgtcg
     2041 ccgcctttac gccggcgctg ccgtgggccg tcaatccggt cgagcagcaa cgcgggcggc
     2101 gcattgccgc gacagccgtt gttctcttcg tgctgttctc ggcgctcatg acggtgttcg
     2161 atcccccgcc gatcgatcgg gccgaggccg agaaactgcc gccgcgactg gccaaactcg
     2221 tccttgagaa aaaggaagcg ccgccgccca aacccgagcc ggtgatcgag aagcccaagg
     2281 aagaaccgaa gcccgaggtc aaggaagaga cgccgaatga agaacccaag gtaaagccga
     2341 ccgagcagca ggtcgagaag gcgcgcgaga aggccaagaa atccggtctg ctggcgatga
     2401 aggaccagct cgcggcgctg cgcgatctga gcactgcctc gtccttgcgt cagccgcagg
     2461 ccaaggtcgg cgccgaaggc acggagcagc gcgtcgagcg cgacctgatc ggccgcaagg
     2521 cgaccagcgg ctcgggcggc gtcgccacca cggccgtcgc gcacagcggg ggcggcacgc
     2581 tggccgggcg cacgacgacg caggttgagg ttccggtcag cgccggcgaa agcctcgccg
     2641 aaacgcgggc caaggcccgt tcgccg
//
LOCUS       DATDAA010000326         2244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_198938,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000326 DATDAA010000000
VERSION     DATDAA010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198938"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VIS73_09990"
     CDS             <1..191
                     /locus_tag="VIS73_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763523.1"
                     /translation="SPGRSGALFDAGTRALNGVVGAGERLSASALEVEITQRCPADDA
                     GTRGRARERGIRQIGRAD"
     gene            complement(204..635)
                     /locus_tag="VIS73_09995"
     CDS             complement(204..635)
                     /locus_tag="VIS73_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5763524.1"
                     /translation="MSAESDVRRASEQFYAALNRMGNGEKGAMADAWSHAPAVTAMHP
                     IGGRSTGWDAVRDSFDEVAGMAADAKIGLKDQFIQVHGDLAYELGVEHGTFKMAGQPV
                     SLEHRVTNIYRREAGGWKLIHHHTDTSPGMLEVLSRLQSAS"
     gene            complement(709..1035)
                     /locus_tag="VIS73_10000"
     CDS             complement(709..1035)
                     /locus_tag="VIS73_10000"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEY5763525.1"
                     /translation="MSLYMTRARYTPEACKGMLSNPGDRASAAKAMFEAAGIKMHHIW
                     MTANGDVVTVVEGNAVSGAAVSMVVMASGAFTEVDSIELITPEQQLEAMKLGGSVASK
                     YRAPGK"
     gene            complement(1149..1697)
                     /locus_tag="VIS73_10005"
     CDS             complement(1149..1697)
                     /locus_tag="VIS73_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763526.1"
                     /translation="MLGAAAALAATLVPLRRARGHTPYRQWDVFRKRYLLILTSRSDA
                     EGDAIGDAWVALLRERLPLSRAMVSRARDMLRVASLLRTDQAKLAVLSHADARAMSAG
                     EPPHEEYGPMPLQLMIDNGTHVLVVRGDFPLHHGYLITVSLLERAQQLHLSVPLEGKF
                     GMAVHPGARAAALGEELDEPRS"
     gene            complement(1784..>2244)
                     /locus_tag="VIS73_10010"
     CDS             complement(1784..>2244)
                     /locus_tag="VIS73_10010"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEY5763527.1"
                     /translation="GDVHYAQGDGEVAGTAIEMGAVVTVQTEIRKGMAASVKQPQFEG
                     GSQLKQLEPDAFHATVGYPFKGAGEIPPQWAYLGSEKLKPLTQLSNDLTMAARNSLLE
                     MIAWLQKTKGLTSEQAYVLTSVACDLRVGNVVDVPNYTVSTICPLEIFKQ"
BASE COUNT          379 a          745 c          700 g          420 t
ORIGIN      
        1 gcagccctgg aaggtcgggg gcgctttttg acgcaggcac gagggcctta aacggcgtcg
       61 tcggtgcggg cgagcgcttg agcgcgagcg cgctcgaggt cgagatcacg cagcgttgcc
      121 cggccgacga cgctggcacc cgcggccgtg cgcgcgaacg cggcatcagg cagatcggcc
      181 gcgcggatta gggcaactcg ccgctacgag gcagattgca ggcggctcaa cacctcgagc
      241 attcccggcg acgtgtccgt atggtggtgg atcagcttcc atcccccggc ctcgcgccgg
      301 tagatgttgg tgacgcgatg ctcgaggctg acaggctgtc ccgccatctt gaacgtaccg
      361 tgctcgactc ccaattcgta ggccaaatcg ccgtgcacct gtatgaactg gtccttcagg
      421 ccgatcttgg cgtctgccgc catgcccgcg acttcgtcga atgagtcccg caccgcgtcc
      481 cagcccgtgc tgcggccgcc gatgggatgc atcgcggtca ccgcgggagc gtgggaccag
      541 gcgtcggcca ttgcgccctt ctcgccgtta cccatccgat tcaaggcagc atagaactgc
      601 tcggacgccc tgcgaacgtc actttccgct gacataatca gtctcctttc aggcgttcaa
      661 aggcgcggtc gctgcgcaac ggaatcgccg atgggatcgg cgtcgcgcct actttccggg
      721 tgcgcggtac ttcgacgcga cgctgcctcc caacttcatc gcctcgagct gctgttccgg
      781 ggtaatcagc tcgatcgaat cgacctcggt gaatgcgccg ctcgccatga ccaccatgct
      841 taccgcggcc cccgacaccg cgttgccttc gaccacggtg accacgtcgc cgtttgccgt
      901 catccagatg tggtgcatct tgatgccggc ggcttcaaac atcgctttgg ctgcgcttgc
      961 gcggtccccc ggattggaga gcatgccttt acaggcttca ggcgtgtagc gcgctcgcgt
     1021 catataaaga ctcatgatga atacctcctc ttgatcctcg caaaaccgct tgtcggcgaa
     1081 gaatccgacg cattcaatct acacccgggg gcactcgcgg gcaatgtccg gtttgcggcg
     1141 cgaggcgcct aggatcgcgg ctcgtcgagc tcttcaccga gcgcggcggc gcgcgcgccg
     1201 ggatgaaccg ccatgccgaa cttgccttca agcggcacgc tcagatgcag ctgctgcgcc
     1261 cgctcgagca gcgacaccgt gatcaggtaa ccgtggtgca gcggaaaatc accgcgcaca
     1321 accagcacgt gggtgccgtt gtcgatcatc agctgcaacg gcatgggccc gtattcttcg
     1381 tgtggtggct cgccggcaga catggcccgt gcatcggcgt gcgacaacac cgcaagcttt
     1441 gcctgatcgg tccggagcag gctggccacc cgcagcatgt cccgcgcgcg actcaccatg
     1501 gcgcgcgaca gcggcaggcg ttcgcgtagc agcgcaaccc atgcgtcgcc gatcgcgtcg
     1561 ccttccgcgt cgcttcgcga cgtcagaatg aggaggtagc gtttgcggaa gacatcccac
     1621 tgccggtaag gcgtatgccc ccgcgcccgg cgcaagggta caagcgtggc ggccagcgca
     1681 gcggcggcgc ccagcaagag tcgtcttcgg cgcgcgtcgg gctgaaaaaa aagcgagcac
     1741 tgggctcgct ttttctcgta acgcctgtgc cggtgcggcg acgtcactgc ttgaagattt
     1801 ccaatgggca aatggtcgag acggtgtagt tcggcacgtc aaccacgttg ccgacgcgca
     1861 ggtcgcaggc cacgctcgtc aggacatacg cctgctcgct ggtcagcccc ttcgtcttct
     1921 gcagccaggc gatcatctca agcaaggaat tgcgcgcggc catcgtcagg tcgttcgaca
     1981 gctgggtcag gggcttgagc ttctcggagc cgaggtaagc ccactgcggc ggaatttcgc
     2041 cggcgccctt gaagggatag ccgaccgtcg catgaaacgc atcgggctcg agctgcttga
     2101 gctgcgaacc gccttcgaat tgcggctgct tcacgctcgc cgccatgcct tttcgtattt
     2161 cggtctgcac cgtcacgacg gcgcccattt cgatcgccgt gccagcgact tcgccgtcgc
     2221 cctgcgcata gtgaacgtca ccga
//
LOCUS       DATDAA010000327         8343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_304476,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000327 DATDAA010000000
VERSION     DATDAA010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304476"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..388)
                     /locus_tag="VIS73_10015"
     CDS             complement(161..388)
                     /locus_tag="VIS73_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEY5763528.1"
                     /translation="MTGHWRTIDARGLQPPEPFVLTMEALDGLPAGASLLVLLHREPF
                     PLYQVLDEQGYRRDSRWHDDGTFEIVIHRDR"
     gene            complement(391..837)
                     /locus_tag="VIS73_10020"
     CDS             complement(391..837)
                     /locus_tag="VIS73_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY5763529.1"
                     /translation="MTVLRDLMADDHRRCDELFAQAEQAAAKGDWAATAAAFAGFAQA
                     LERHLAAEESDLFPAFEAATGMTMGPTQMMRHEHQEMRALLVETGEAIERRDDEAFRG
                     LTETLLIMMQQHNLKEENILYPMCDANLAATSGQIAGRLRALIGHG"
     gene            complement(1396..3093)
                     /locus_tag="VIS73_10025"
     CDS             complement(1396..3093)
                     /locus_tag="VIS73_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5763530.1"
                     /translation="MVSLEETGRRIEQQIDPLGVMTSLVNAQMAWMLHPVELSHALTG
                     LAGDLFKLQTHIAHRALGLSSDDVIAPNTDDARFADPVWTESASWDIVKEWYLGLTHR
                     LQNMAYETPGLSEKERRRAAFWSRKWLNAMAPTNFLVTNPVAMRKFVETHGESLRAGW
                     DHFLRDLKAKNVLMVDPEAFKVGVDLATTPGAVVARNRLVEIIRYTPTTETVLSIPIV
                     IVTPWINKFYILDLNTRKSLVRYLVAQGFTVFITSWKNPDAGMSEVSFDDYLIEGVHF
                     AVETAREICKVPQVHAAGYCIGGTTLATYMAWANRHFGEPDKVPVAHWTLFTTLTDFG
                     KPGDIEVFIDEPAIEWLEQSMKQQGYLDGQQMASSFRLLRSNTLIWHYVVHSYLYGEP
                     LPAFDVLFWNMDTTRMPERMHSFYLREFYLHNKLIHRDALTLAGEPIYLGRIEQPLYA
                     VTAEDDHIAPWRQCYRIRKAVAGPMRFVLSSSGHILGIVNPPVNPPKRKYRVADPERG
                     DKAEDFLEDATAHAGSWWEDWTRWLSERSGARVKPPPMAREGNPVLCDAPGEYVLAK"
     gene            3273..3716
                     /locus_tag="VIS73_10030"
     CDS             3273..3716
                     /locus_tag="VIS73_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5763531.1"
                     /translation="MFKHILIPTDGSELSQKNAQRAISFAKEAGARVTAFHAMPEYPV
                     TYYGEGALIDPTTPEKFSEMMNKQADEHLAAVAAQCKAAGVACETMKSTSDVPYEAII
                     AAAEKAGCDLIFMASHGRRGISALLLGSETHKVLTHTTIPVLVHR"
     gene            complement(3717..4208)
                     /locus_tag="VIS73_10035"
     CDS             complement(3717..4208)
                     /locus_tag="VIS73_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEY5763532.1"
                     /translation="MSALVVFAWGNPSRGDDALGPLLAERLQRLPAVRSGEVALVEDF
                     QLQVEHAMDLGSRSLALFIDAASGLDLPFVFSAITPRFQPTHSTHAIAPESVLAVAGD
                     VLGEAPPPAFLLAVSGQDFDLGAPLSPAAQANLEAAWGFIGELLAAPDAAAWQARAGA
                     PAG"
     gene            complement(4213..5700)
                     /locus_tag="VIS73_10040"
     CDS             complement(4213..5700)
                     /locus_tag="VIS73_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEY5763533.1"
                     /translation="MTQLETASNPETLRRVAIEPVSRVEGHGKVTLLLDEDNRVHQVR
                     LHIVEFRGFEKFIQGRPYWEVPVMVQRLCGICPVSHHLAASKAFDAIVGATTLTPTAE
                     KNRRLMHYGQMLQSHALHFFHLSSPDLLFGFDSDAARRNIVGVAAAHPEVAKQGILLR
                     KFGQEVIRMTAGKRVHGTGAIPGGVNKSLSREERAELQKDCYQMVSWSRDAVQLIKKL
                     HKANAAEYDRFGYFESNLMSLVRADGAMDFYHGGLRAREANGKPIFDGVDYRGYSELI
                     HEEVKPWSYMKFPFLTSLGKEQGWYKVGPLARVQNCDYIPTPLAEVERREFVAHGQGK
                     LVHAPLAYHWARMIEMLHAAETIKDLLHDNDIVGSDLMAGGERQREGVGVIEAPRGTL
                     FHHYRVDDNDLVTMCNLIVSTTNNNQPMNAAVRQVARQYLDGKELNEGLLNHVEVAIR
                     AFDPCLSCATHALGKMPLEVELVDAEGACLDRLWRGSDGRATRGD"
     gene            complement(5723..6268)
                     /locus_tag="VIS73_10045"
     CDS             complement(5723..6268)
                     /locus_tag="VIS73_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HEY5763534.1"
                     /translation="MSETKKLRVATTSLAGCFGCHMSFLDIDERILELVELVEFDRSP
                     LTDIKHCGPCDIGLIEGGICNAENVHVLREFRANCKTLIAVGACAVNGGLPAQRNHLD
                     LRDCLQEVYQHSLGVVDGQVPNDPELPLPLDKVHPIHEIVRIDYFIPGCPPSGDAIWK
                     FLTDLIAGRTPRLSHPMLHYD"
     gene            complement(6265..6987)
                     /locus_tag="VIS73_10050"
     CDS             complement(6265..6987)
                     /locus_tag="VIS73_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY5763535.1"
                     /translation="MSGQFMLDGQPVEFEEGQTILHAALAADHFIPHLCHHPEFKPHG
                     SCKLCTVRANGRYVSACTMAAKEGMEVENNTPELGDKRRTLLQMLFVEGNHFCPSCDK
                     SGNCMLQALAYELEVMTPNFPQFFPQRPVDASHPDVILDFNRCILCELCIRASRDVDG
                     KSVFALSGRGITKHLIVNAESGHLGDTDFSIADKAAHVCPVGVIQPKRTGFAVPIGSR
                     QYDARPISAQIDELTSRKAEQK"
     gene            complement(6984..>8343)
                     /locus_tag="VIS73_10055"
     CDS             complement(6984..>8343)
                     /locus_tag="VIS73_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY5763536.1"
                     /translation="ELVRARTPVEQWPAEYFEVVDNICRRDVLLDSPLKPGEALRAAV
                     ARGAEAMLAELKLSNLRGRGGAGFTTAVKWDSCRQAPVAEKHQRVVVCNADEGEPGTF
                     KDRVLLTRHADLVFEGMTIAALTIGANKGYLYLRGEYLYLLPALQANLERRRREGMLG
                     KGILGTVGFDFDIDIHLGCGAYVCGEETALIESLEGKRGIPRVRPPFPVTNGYLGRPT
                     IVNNVETLAKAALIAVSGGAWFAGLGTKQSTGTKVLSISGDVERPGIYEYPFGVTVEQ
                     VLRDCGAGNAQAVQVSGASGVCLDANEFRRRIAFEDVPTAGAFMVFGPDRDLFEVARN
                     FAHFFAHESCGFCTPCRVGTSLVMNCLDKIADGAGTQHEINEIFKLHRLMHAGSHCGL
                     GQAACNPVIDTLTKFRPAYERRLRSLDFAPAFDLDKALAAARQMTGRDDADAHFANEA
                     NK"
BASE COUNT         1488 a         2645 c         2754 g         1456 t
ORIGIN      
        1 tgcacgaagg ccagcgtcgc cggcgcccag cgcgaggcac tcgctgccgg gccgacgatc
       61 agcagcagca atccggcagc cgcgaggaac aacggcgccg tgagaaagaa gcgcagcggc
      121 accgtgatcg gcggcgcctg atcgaagccg agctgcggcg tcatcggtcg cggtggatga
      181 cgatctcgaa ggtaccgtcg tcgtgccagc ggctatcgcg acgatagccc tgctcgtcga
      241 gcacctgata gagcgggaag ggttcgcgat gcagcagcac cagcagcgaa gcgccggccg
      301 gcagtccgtc gagcgcctcc atggtcagca cgaagggctc gggcggctgc aggccgcggg
      361 cgtcgatcgt gcgccagtgc ccggtcactt tcagccgtgc ccgatcagcg cccgcagccg
      421 gccggcgatc tggccggacg tcgccgcgag gttcgcgtcg cacatcggat agaggatgtt
      481 ctcttccttg aggttgtgct gctgcatcat gatcagcagc gtctcggtaa gcccgcggaa
      541 cgcttcgtcg tcgcgccgct cgatcgcctc accggtctcg accagcaacg cgcgcatttc
      601 ctgatgctcg tggcgcatca tctgcgtcgg ccccatcgtc atcccggtcg ccgcctcgaa
      661 agccggaaac aagtccgact cttcggctgc caaatgccgt tcgagcgcct gcgcgaagcc
      721 ggcaaaggca gcggccgtcg ccgcccagtc gcccttcgcg gccgcctgtt cggcctgcgc
      781 gaacaactcg tcgcagcggc ggtgatcatc ggccatcagg tcgcgcaata cggtcatcgg
      841 ttactccatc gggggcgtgg aaggcgggcg cagtgtgggg catcgccacg cctgaggtca
      901 ttgactcgaa tcaaattcgg gctgcgtcgc gatggagtaa ccgctacctg ctgtgcccct
      961 ccgctgactg agtctttacc ccgataaatg cactcacgtc atgatcaagt cggctttagc
     1021 cgcggtcgat caacgttgat gcaagccatt tgtttccaaa tgccccacaa accacggcaa
     1081 agcacaggta ggtagcaagg ttcagcacga cgagcacttc actacccaga cccgccgcca
     1141 atccgtcaag cacttcaaga gcgttgagcg cgccaactag catgctgcca ggaacccata
     1201 agcgaatcta gagcgcccag agggggccaa acaacagggc cagccaagaa aagcccttct
     1261 gtacatcaac ccgggatcca gacggatgag caaagctctt tattcggtct ttcatcggtt
     1321 acttatgggg tctctgcgcc aagcgaagga tgcgcgacag tcgagccagc gagaacgcgg
     1381 atgcttgcag cgatcctact tcgcgagaac atactcgccc ggcgcatcgc atagcaccgg
     1441 gttcccctcg cgcgccatcg gcggcggctt gacgcgcgcg ccgctgcgct cggataacca
     1501 gcgcgtccag tcttcccacc agctgccggc atgcgccgtg gcgtcttcga ggaagtcttc
     1561 ggccttgtcg ccgcgctctg gatcggcaac gcggtactta cgcttgggcg gattgaccgg
     1621 cggattgacg atgccgagga tgtgcccgga actcgaaagg acgaaacgca tggggccggc
     1681 gacagccttg cggatgcggt agcactggcg ccacggcgcg atgtggtcgt cctcggcggt
     1741 gaccgcgtag agcggctgct cgatgcgacc gaggtagatc ggctcgccgg cgagcgtcag
     1801 cgcatcgcgg tggatcagct tgttgtgcag gtagaactcg cgcaggtaga aactatgcat
     1861 gcgttcgggc atgcgcgtcg tgtccatgtt ccagaacagc acgtcgaatg ccggcagcgg
     1921 ctcgccgtag aggtagctgt gcaccacgta gtgccagatc agcgtattcg agcgcagcag
     1981 gcggaaagac gaggccatct gctggccgtc gagatagccc tgctgcttca tcgactgttc
     2041 aagccactcg atcgccggct cgtcgatgaa gacctcgatg tcgccgggct tgccaaaatc
     2101 ggtgagcgtg gtgaacagcg tccagtgagc gaccggcacc ttgtctggct cgccgaagtg
     2161 ccggttggcc caggccatgt aggtcgccag cgtggtgccg ccgatacagt agccggccgc
     2221 atgaacctgc ggcaccttgc agatctccct agccgtctcg acggcgaagt gcacaccctc
     2281 gatcaggtag tcgtcgaaag agacctcgga cattccagcg tctgggttct tccagctggt
     2341 gatgaagacc gtgaagccct gcgcgacgag gtagcggacg agactcttcc tggtgttgag
     2401 gtcaaggatg tagaacttgt tgatccaagg cgtgacgatg acgatgggga tcgagaggac
     2461 cgtctctgtg gtcggcgtgt aacggatgat ctcgacgagg cggttgcgcg cgaccactgc
     2521 gcctggcgtc gtcgccaggt cgacgccgac cttgaaggcc tcaggatcga ccatcagcac
     2581 gttcttcgcc ttgaggtcac gcaggaagtg atcccagccg gcacgcaggc tttcgccgtg
     2641 cgtctcgacg aacttgcgca tcgccaccgg gttggtgacg aggaagttgg tcggcgccat
     2701 cgcattgagc cacttgcgcg accagaaggc cgcgcggcga cgttccttct ccgacagtcc
     2761 gggcgtctcg taggccatat tctgcagccg gtgcgtcagc ccgagatacc attctttgac
     2821 gatgtcccag ctcgccgact cggtccacac cggatcggcg aagcgcgcat cgtcggtgtt
     2881 cggtgcgatc acgtcgtcgc tggaaaggcc cagcgcgcgg tgggcgatgt gcgtctgcag
     2941 cttgaagagg tcgccggcga ggccggtcag cgcgtgcgaa agctctaccg ggtgcagcat
     3001 ccaggccatc tgcgcgttga ccagcgaggt catgacgcct agcggatcga tctgctgctc
     3061 gatccgccga cccgtctctt ccagcgaaac catgcctagg acatcgccat cggcgtggtt
     3121 gcggcccgac cgtttcttgc catcagtcat ggcgactctc ccggtgaaga caacgctagt
     3181 gtagggccaa gcctggcgcc gcaacagtcg gcaaaccact gccgggcggc tgcgctatag
     3241 tggaatcgtg gacaaaccga gggaggctcg ccatgttcaa acacatcctg atcccgaccg
     3301 acggctcgga actctcgcag aagaatgcgc agcgggcgat cagctttgcc aaggaggccg
     3361 gcgcccgcgt cacggccttc cacgcgatgc cggaataccc ggtgacctac tacggcgaag
     3421 gcgcgctgat cgatccgacg acgcccgaga agttctccga gatgatgaac aagcaggccg
     3481 acgaacacct cgcggcggtg gccgcacaat gcaaggcggc cggtgtggcc tgcgagacga
     3541 tgaagtcgac cagcgacgtg ccctacgagg cgatcatcgc ggcagccgaa aaagccggct
     3601 gcgacctgat cttcatggcc tcgcacggcc ggcgcggcat ctcggcgctg ctgctcggca
     3661 gcgagacgca caaggtgctg acgcacacga cgattccggt gctcgtgcac cgctgatcag
     3721 ccagccggcg caccggctcg tgcctgccac gccgctgcat cgggcgctgc cagcagttcg
     3781 ccgatgaagc cccaggccgc ttccaagttc gcctgcgccg ccggcgacag cggcgcccct
     3841 agatcgaagt cttgaccgct gacggcgagc agaaatgccg gcggcggcgc ctcgccgagc
     3901 acatcaccgg cgaccgcgag cacgctctcg ggggcgatcg cgtgcgtcga atgggtgggc
     3961 tggaagcgcg gcgtgatcgc cgaaaagacg aagggtaaat cgaggccgct ggccgcgtcg
     4021 atgaacagcg caagcgagcg gctgcccaga tccatcgcat gctcgacctg caactggaaa
     4081 tcctcgacca gcgcaacttc gccggaacgc acggccggca atcgctgcag gcgttcggcg
     4141 agcagagggc cgagcgcgtc gtcgccgcgg ctagggttgc cccaggcaaa gacgaccagt
     4201 gcgctcaccg gctcagtcgc cgcgcgtcgc ccgcccgtcg ctgccgcgcc agaggcgatc
     4261 gaggcaggcg ccctcagcgt cgacgagttc aacttcgagc ggcatcttgc ccagcgcgtg
     4321 cgtggcacag gagaggcaag ggtcgaaggc gcggatagcc acctcgacgt ggttgagcag
     4381 gccctcgttg agttccttgc cgtcgaggta ctgccgcgcg acctgacgaa ccgcggcgtt
     4441 catcggctgg ttgttgttgg tggtcgagac gatcaggttg cacatggtaa cgagatcgtt
     4501 gtcgtcgacg cggtagtggt ggaacagcgt gccgcgcggc gcctcgatca cgccgacgcc
     4561 ctcgcgctgg cgctcgccgc cggccatcag atcgctgccg acgatatcgt tgtcgtggag
     4621 caggtccttg atcgtttccg ccgcatgcag catttcgatc atccgcgccc agtggtaggc
     4681 cagcggcgcg tgtaccagct tgccctggcc gtgggcgaca aactcgcggc gctcgacctc
     4741 ggccagcggc gtcgggatgt aatcgcaatt ctgcacgcgc gccagcggcc ccaccttgta
     4801 ccaaccctgt tccttgccca atgaggtgag aaacggaaac ttcatgtagc tccacggttt
     4861 cacttcctcg tggatcagct cgctgtagcc gcggtaatcg acgccgtcga aaatcggctt
     4921 gccgttcgcc tctcgggcgc gcaggccgcc gtggtagaaa tccatcgcgc catcggcacg
     4981 gaccaggctc atcaggttcg attcgaagta gccgaagcgg tcgtactcgg cggcgttggc
     5041 cttgtgcagc ttcttgatca gctgcacggc atcgcggctc cacgacacca tctggtagca
     5101 atccttctgc agttcggccc gctcttcgcg cgacagcgac ttgttgacgc cgccgggaat
     5161 cgcgccagtg ccatgcaccc gcttgccagc cgtcatgcgg atcacttcct ggccgaactt
     5221 gcgcaacagg atgccctgct tcgccacctc gggatgcgcc gcggcgacgc cgacgatgtt
     5281 gcgccgggcg gcgtcgctgt cgaagccgaa cagcagatcg ggcgaggaga gatggaagaa
     5341 atgcagtgca tgcgactgca gcatctgccc gtagtgcatc agccggcgat tcttttccgc
     5401 cgtcggcgtc agcgtcgtcg cgccgacgat cgcatcgaag gccttgctcg ccgccagatg
     5461 gtgcgagacc gggcagatgc cgcacaggcg ctgcaccatc accggcactt cccagtacgg
     5521 ccggccctga atgaatttct cgaagccgcg gaattcgacg atatgcaggc gaacctgatg
     5581 aacgcggttg tcctcgtcga gcagcagcgt caccttgccg tgtccctcga cgcggcttac
     5641 cggttcgatg gcaacgcggc gaagcgtctc gggattgcta gcggtttcca gttgggtcat
     5701 cgtcatcagc cttcgaattc ggtcaatcgt agtgcagcat cgggtgggag agccgcggcg
     5761 tccgcccggc gatcaaatcg gtcaggaact tccagatcgc gtcgcccgac ggtgggcacc
     5821 cggggatgaa atagtcgatg cggacgatct cgtggatcgg atgcaccttg tcgagcggca
     5881 gcggcagctc ggggtcgttg ggcacctggc cgtcgaccac gccaaggctg tgctggtaga
     5941 cctcctgcag gcagtcgcgc aagtcgaggt ggttgcgctg cgccggcagt cctccgttga
     6001 cggcgcaggc accgacggcg atcagcgtct tgcagttggc gcggaactcg cgcagcacgt
     6061 gcacgttctc ggcgttgcag atgccacctt cgatgagacc gatgtcgcag ggaccgcagt
     6121 gcttgatgtc ggtgagcggc gagcgatcga actcgacgag ttcgacgagt tcgaggatgc
     6181 gctcgtcgat gtcgaggaag gacatgtggc agccgaagca gccggcaagc gaggtcgtcg
     6241 cgaccctgag tttcttcgtt tcgctcattt ctgctcggcc ttccgcgagg tgagttcgtc
     6301 gatctgggcg ctgatcggcc gcgcgtcgta ctggcggcta ccgatcggca cggcgaaacc
     6361 cgtgcgcttg ggctggatca cgccgaccgg gcagacgtgc gcagccttgt cggcgatcga
     6421 gaaatcggtg tcgccgagat gacccgattc ggcgttgacg attaagtgtt tggtaatgcc
     6481 gcggcccgag agcgcgaaga cgctcttgcc atcgacgtcg cggctggctc ggatgcacag
     6541 ctcgcagagg atgcagcggt tgaagtcgag gatcacgtcg gggtgcgagg cgtcgaccgg
     6601 ccgctgcggg aagaactgcg ggaagttcgg cgtcatcact tccagttcgt aggccagcgc
     6661 ctgcagcatg cagttaccgc tcttgtcgca cgagggacag aagtgattgc cttcgacaaa
     6721 gagcatctgc agcagcgtgc gccgcttgtc gccgagttct ggggtgttgt tctcgacctc
     6781 catgccctcc ttcgcggcca tcgtgcaggc cgaaacgtag cggccgttgg cgcggaccgt
     6841 acacagcttg cagctgccgt gcggcttgaa ctccgggtga tggcagaggt gcgggatgaa
     6901 gtggtcggcc gccagcgccg cgtgcaggat cgtctggcct tcctcgaatt cgacgggctg
     6961 tccgtcaagc atgaactgtc cactcatttg ttcgcctcgt tcgcaaagtg ggcgtctgcg
     7021 tcgtcgcggc cagtcatctg ccgcgcggcg gcgagcgcct tgtcgagatc aaaggccggc
     7081 gcgaaatcga gcgagcgcaa gcgccgctcg taggccggcc gaaacttggt cagcgtatcg
     7141 atcaccggat tgcatgccgc ctgcccgagg ccgcaatggc tgccggcgtg catcaggcga
     7201 tggagcttga agatctcgtt gatctcgtgc tgggtaccgg cgccgtcggc gatcttgtcg
     7261 aggcagttca tcaccagcga ggttccgacg cgacagggcg tgcagaagcc acagctctcg
     7321 tgggcgaaga aatgggcgaa gttgcgcgcc acctcaaaca ggtcgcggtc ggggccgaag
     7381 accatgaagg cgccggcagt cggcacgtct tcgaaggcga tgcggcggcg gaactcgttg
     7441 gcatcgagac agacgcccga agcgccgctg acctgcaccg cctgcgcgtt gccggcgccg
     7501 cagtcgcgca gcacctgctc gacggtgacc ccgaaagggt actcgtagat gcccggccgc
     7561 tcgacgtcgc ccgagatcga tagcaccttg gtgcccgtcg actgcttggt gccgaggccg
     7621 gcaaaccagg caccgccgga aacggcgatc agcgccgcct tggccagcgt ctcgacgttg
     7681 ttgacgatgg tcggccgccc gaggtagccg ttggtaaccg ggaaaggcgg acgaacgcgc
     7741 gggatgccgc gcttgccttc gagcgattcg atcagcgcgg tttcctcgcc gcagacatag
     7801 gcgccgcagc ccaagtggat atcgatgtcg aagtcgaagc cgacagtgcc gagaatgccc
     7861 ttgccgagca tgccctcgcg gcggcggcgt tcgagattgg cctggagcgc cggcaacaga
     7921 tagaggtatt cgccgcgcag gtagaggtag cctttgttgg cacctatcgt cagcgcggcg
     7981 atggtcatgc cttcgaagac gagatccgca tggcgcgtca gcagcacgcg atccttgaag
     8041 gtaccgggtt cgccctcgtc ggcattgcaa acgacaacac gctggtgctt ttcggcgact
     8101 ggcgcctggc ggcaggagtc ccatttgacg gcagtggtga agccggcgcc gccgcggccg
     8161 cgcaggttgg agagcttcag ctcggcgagc attgcctcgg ccccgcgcgc gacagcggcg
     8221 cgcagcgcct cgccgggctt gagcggcgag tcgagcagga cgtcgcggcg acagatgttg
     8281 tcgacgacct cgaaatactc ggccggccat tgctcgaccg gcgtgcgcgc ccgcaccagt
     8341 tcg
//
LOCUS       DATDAA010000328         2426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_129304,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000328 DATDAA010000000
VERSION     DATDAA010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129304"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VIS73_10060"
     CDS             complement(<1..568)
                     /locus_tag="VIS73_10060"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY5763537.1"
                     /translation="MRVKTLLLAASASMAPFAGPAQEPAVGAEFRVDSDASWIRILAW
                     PDGPLKRFGHHHVISHHGISGSVLVAEDPLDSTFSLEISVADLVVDDPESRALEGAEF
                     QGEVPQEDVEGTRANMLGERLLHGEAFPTIQIRSASIEGSLPEVEIVATLVVKGAENT
                     LRFPARIELTDDALVARGEIELAHAALGL"
     gene            724..1329
                     /locus_tag="VIS73_10065"
     CDS             724..1329
                     /locus_tag="VIS73_10065"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY5763538.1"
                     /translation="MPASIEHTHPTQRAITMRVSWIILPTLFSGAAFAEAEASEWQLV
                     EGSSTVRFIGVQEGSAFRGRFENFTAIIDFDPADPAAGRIVGVVKMDSAKTGDAERDA
                     TLLEEDWFNPAVHPDSSFESERIERLDDGSYAAHGQLTIVGVSQPVTMAFEFQVEDST
                     AHFSGSFDILRLDFGVGWDATNWIDDEVGVQVELDLQRQTR"
     gene            1339..>2426
                     /locus_tag="VIS73_10070"
     CDS             1339..>2426
                     /locus_tag="VIS73_10070"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5763539.1"
                     /translation="MAKQGSLQPKSVSKREFLEALTAFGGVSAVLTALDGWGMGVASA
                     AEAPPDLAGRAEGTSVLILGAGLSGMTAAYELGLRGYDCRILEARPFAGGRCQSSRAG
                     FTTTTVGGETRTCEFDEGHYFNHGPWRLPSYHHAVFHYVRKFGVPMEIMVQENDLAYV
                     QYDDADGPLAGRRLRRREIKADMRGYTAELLAKLADQGALDKELTYQDRERLVDYLIL
                     QGFLDARDLSYKGTVHRGLAAFDAMSPGSPDTASEALAFKDLLQSGLGNIFQGVTTID
                     HPTTMYQPVGGMDQIARAFEREVGEMITFNAEVQELRQGDDGVTVPYRDTASGEIRVA
                     RADYCITTIPLGILSKLPADLSDRCKEA"
BASE COUNT          450 a          763 c          780 g          433 t
ORIGIN      
        1 acaggccgag cgcggcatgc gcaagttcta tttcgccgcg cgcgacgagc gcgtcgtccg
       61 tcaactcgat gcgggccgga aaccttaggg tgttttccgc gcctttcacg acgagcgtgg
      121 cgacgatctc gacctccggc aggctgccct cgatcgaagc ggacctgatt tgaatcgtcg
      181 gaaacgcctc gccgtgcagc agccgctcac cgagcatgtt ggccctcgtg ccctcgacgt
      241 cctcctgcgg gacctcgccc tggaactccg cgccttcgag ggcccgcgat tcgggatcgt
      301 cgaccacgag atcggccacg gaaatttcca ggctgaaggt cgaatcgagc ggatcttcgg
      361 caaccagcac ggagcccgag atgccgtggt gcgaaatcac gtggtgatga ccgaagcgct
      421 tcagggggcc gtccggccag gcgaggatac ggatccagct ggcgtcgctg tcgacgcgaa
      481 actccgcgcc caccgccggt tcctgcgcgg ggccggcaaa cggcgccatg cttgcggacg
      541 ccgccagcag tagggttttt acgcgcatgc gctgttctcg ctgacttaac aaatcttttc
      601 gccgggcgga tcttactatg ttgtctaatc gcatggcacc gccgtcacga acaacgaaat
      661 cgacggcggt acacgattga aaatcgcttc ctcagccgtc aggatacacg ggcgccggcc
      721 cggttgccgg cgtcaatcga gcatactcat ccgactcaga gggccataac gatgcgggta
      781 agctggatca ttttaccgac gcttttttca ggcgctgcgt tcgcggaagc cgaagcctcc
      841 gaatggcagc tcgtcgaggg ctcgagcacg gtgcgcttta tcggcgtgca ggaaggatcc
      901 gctttccggg gacggttcga aaatttcact gcgatcatcg acttcgatcc cgctgacccc
      961 gccgccggca ggatcgtcgg cgtggtcaag atggattccg caaagaccgg ggacgccgag
     1021 agagatgcaa cgctcctgga ggaagactgg ttcaatccgg cggtccaccc ggattcgagc
     1081 ttcgaatccg agcgcatcga gcggctggac gacggctcct acgccgccca cggacagctg
     1141 accatcgtcg gcgtgagcca gccggtcacg atggccttcg aattccaagt cgaggattcg
     1201 accgcgcatt tttccggcag cttcgacatc ctgcgcctgg actttggcgt tggctgggat
     1261 gcgaccaact ggatcgacga cgaggtcggc gtccaggtgg aactcgattt gcagcgacag
     1321 acccgatagg gaaatcccat ggcgaaacaa ggatctctgc agcctaaatc tgtcagcaag
     1381 cgtgagtttc tcgaggcgtt gaccgccttt ggcggcgtat ccgccgtatt gacggcgctc
     1441 gatggctggg gcatgggcgt tgcctccgcc gcggaggcgc cgcccgatct tgccgggcgc
     1501 gccgaaggca ccagcgtgct gatcctgggc gccgggctct ccggcatgac cgcggcctac
     1561 gagctcggcc ttcggggtta cgactgccgg atcctggagg cccgaccttt cgccgggggc
     1621 cgctgccagt cctcgcgcgc aggattcacc acgacgacgg tgggcgggga aacccgtacc
     1681 tgcgagttcg acgagggcca ctatttcaac cacgggccct ggcgcctgcc gagttatcac
     1741 cacgcggtat tccactacgt ccgcaagttc ggcgttccga tggagatcat ggtccaggag
     1801 aacgacctcg cctacgtgca gtacgacgac gcggacgggc ccctggccgg tcgccgcctg
     1861 cgccggcgcg aaatcaaggc cgacatgcgg ggctacacgg cggagctttt ggcgaaactc
     1921 gcggaccagg gggcgctcga caaggagctg acctaccagg acagggaacg gctggtcgac
     1981 tacctcattc tccagggctt cctggatgcg cgggatctga gttacaaggg aaccgtgcac
     2041 cggggcctgg cggcgttcga cgccatgagc cccggcagcc cggatacggc ctcggaggcc
     2101 cttgctttca aggacctgtt gcagtccggc ctcggcaata ttttccaggg cgtcacgacg
     2161 atcgaccatc cgacgacaat gtatcagccc gtcggtggca tggaccagat cgcccgggcc
     2221 ttcgagcgtg aagtgggcga aatgatcact ttcaatgccg aggtccagga attgaggcaa
     2281 ggcgacgatg gcgtcacggt gccctaccgg gatacggcca gcggagaaat ccgggtcgcc
     2341 agggcagact actgcatcac cacgattccg ctgggcatac tatcgaagct gccggcggat
     2401 ctgtcggatc gctgcaagga ggcgat
//
LOCUS       DATDAA010000329         2022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_443828,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000329 DATDAA010000000
VERSION     DATDAA010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443828"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VIS73_10075"
     CDS             complement(<1..485)
                     /locus_tag="VIS73_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEY5763540.1"
                     /translation="MTELRALDGNEAVARVAYAVSEICSIYPITPSSPMAEMADVWSA
                     SGKPNIWGQVPDIIEMQSEGGAAGTAHGALQTGALTTTFTASQGLMLMLPNMYKIAGE
                     LTPTVFHVAARSLAAQGLSIFGDHQDVMAARTTGFGLLAASSVQAAQDLALISHAATL
                     EA"
     gene            complement(482..>2022)
                     /locus_tag="VIS73_10080"
     CDS             complement(482..>2022)
                     /locus_tag="VIS73_10080"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY5763541.1"
                     /translation="HACPAGENIQAWLALAQEGDFEAAWRELVSNNPLPSIHGRVCYH
                     PCEDVCNRAFTDSAVSIHAVERYLGDLALEKGWQVERPGKTSGKRILVIGAGPSGLSA
                     AYHLVRLGHDVEIREAGPVAGGMIHFGIPAYRMPRKELQAEIERIENMGVRITLNHRV
                     EDVLAEKEDGGFDAVFVAVGAHISKKIDIPSREAGKILDAVSFLSSTKQGETPKLGRR
                     VAIYGGGNTAMDAARTAKRLGVEEAMIIYRRDRSSMPAHDFEADEALEEGVVINWLRT
                     IKEIDRSTFKVEIMELDETGRPQPTGKFETLEADSLILALGQDTDTGFLEKVPGIAFE
                     WDHTVIVDDNMMTGHDGIFAGGDMVPSERSVTIAVGHGKHAARHIDAYLRGAKYRRGP
                     RNPVIGHERLHLWYRTHAPKVEQERLPVANRQTNFDEIMQNLSETDALFESQRCLSCG
                     NCFECDGCYGACPEGAVIKLGPGRRYRFDYAVCTGCAVCFEQCPCHAIEMIDEQQVGG
                     MSAL"
BASE COUNT          308 a          646 c          674 g          394 t
ORIGIN      
        1 gcttcgagcg tcgccgcgtg gctgatgagg gcgagatcct gcgcggcctg caccgacgac
       61 gcggccagca ggccgaagcc ggtggtgcgc gcggccatca cgtcctggtg gtcgccgaaa
      121 atcgacaggc cctgcgcggc cagggaacgc gccgcgacgt ggaacaccgt cggcgtgagt
      181 tcgccggcga tcttgtacat gttgggcagc atcagcatca ggccctgcga cgccgtgaat
      241 gtcgtcgtca gcgcgcccgt ctgcagcgcg ccgtgcgccg tgccggccgc accgccctcg
      301 ctttgcattt cgatgatgtc cggcacctgg ccccagatgt tgggtttgcc gctcgcggac
      361 caaacgtcgg ccatctcggc catcggtgac gacggcgtga tcgggtagat ggagcagatc
      421 tcgctgacgg cgtaggcgac acgggcgact gcctcgttgc cgtcgagtgc gcgtaactcg
      481 gtcatagcgc cgacatcccg ccgacctgct gctcgtcgat catctcgatt gcatggcatg
      541 ggcactgctc aaagcacacg gcgcagcccg tgcacaccgc gtaatcgaag cggtagcgcc
      601 tgccgggtcc gagcttgatc accgcgccct cggggcaggc accgtagcag ccgtcgcact
      661 cgaagcagtt gccgcaggac aggcagcgct gggattcgaa cagggcatcg gtctccgaca
      721 ggttctgcat gatctcgtcg aaattggttt gccggttcgc gacgggcaag cgttcctgtt
      781 cgaccttggg ggcgtgggtg cggtaccaga gatgcagccg ctcgtggccg atgaccgggt
      841 tacgcggtcc gcgccggtac ttcgcaccgc ggagatacgc gtcgatgtgc ctggccgcgt
      901 gtttaccgtg gccgaccgcg atcgtcacgg agcgttcgct cggcaccatg tcgccaccgg
      961 cgaaaatgcc gtcatgcccg gtcatcatgt tgtcgtcgac gatgaccgta tgatcccact
     1021 cgaacgcgat gccggggacc ttctcgagga agcccgtgtc agtgtcctgg cccagtgcga
     1081 ggatcaacga gtctgcttcc agcgtttcga atttgccggt cggctgcggc cgtcccgttt
     1141 cgtcgagctc catgatctcg accttgaacg tcgaacgatc gatctccttg attgtgcgca
     1201 gccagttgat gacgacacct tcctcgaggg cctcgtccgc ctcgaagtcg tgcgccggca
     1261 tgctgctgcg gtcgcggcgg tagatgatca tggcctcttc cacaccgagg cgcttggccg
     1321 tgcgcgccgc atccatcgcc gtgtttccgc cgccgtagat ggcgacgcgg cggccgagct
     1381 tcggcgtttc gccctgcttg gtgctggaga ggaagctgac ggcatcgagg atcttgccgg
     1441 cttcgcggct cgggatgtcg atcttcttgc tgatgtgtgc gccgacggcg acgaacacgg
     1501 catcgaagcc gccgtcttcc ttttccgcga gcacgtcttc gacgcggtga ttgagcgtga
     1561 tcctgacgcc catgttctcg atgcgctcga tctcggcctg caattccttg cgcggcatgc
     1621 ggtaagccgg aatgccgaaa tggatcatgc cgccagcgac ggggcctgct tcgcggatct
     1681 cgacgtcgtg gccgagtctt acgagatggt aggcggccga caggccactg gggccggcac
     1741 cgatgacgag gatcctcttg ccgctggtct ttccgggtct ctcgacctgc cagcctttct
     1801 cgagggcgag gtcgccgaga tagcgctcga cggcatggat gctgacagcg ctgtccgtga
     1861 acgcgcggtt gcatacgtcc tcgcacgggt gataacagac acggccatgg atggagggca
     1921 aggggttgtt actgaccagt tcccgccagg cggcctcgaa gtcgccttcc tgcgcgagcg
     1981 ccagccaggc ctggatgttt tcgccggccg ggcaggcgtg gt
//
LOCUS       DATDAA010000330         4115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_479079,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000330 DATDAA010000000
VERSION     DATDAA010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479079"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VIS73_10085"
     CDS             complement(<1..518)
                     /locus_tag="VIS73_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY5763542.1"
                     /translation="MPQVINTNIASLNSQRNLNTSQTALSQALQRLSSGLRINSAKDD
                     AAGLAISERFTTQIRGLNQATRNANDGISLAQTAEGALGEINNNLQRIRELAVQSANA
                     TNSASDRQALDQEVQQRLAEIDRIAAQTSFNGQKVLDGTFGNAAFQVGANVGETISVN
                     LTTSARTNAMGQ"
     gene            734..823
                     /locus_tag="VIS73_10090"
     tRNA            734..823
                     /locus_tag="VIS73_10090"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:768..770,aa:Ser,seq:tga)
     gene            complement(875..1327)
                     /gene="queD"
                     /locus_tag="VIS73_10095"
     CDS             complement(875..1327)
                     /gene="queD"
                     /locus_tag="VIS73_10095"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEY5763543.1"
                     /translation="MLITRRLEFDAGHRIPDHASQCRHLHGHRYAIEVTLSGDIIERD
                     GDAANGMVMDFSEVKSIARQQVVDAWDHAFLVYAGDQAVTAFLATIPGHKTVVLDRVP
                     TAENLARIAFDLLDRAYRDSYGNHLQLEQVRLYETPNCWADARRIDQH"
     gene            1450..2268
                     /locus_tag="VIS73_10100"
     CDS             1450..2268
                     /locus_tag="VIS73_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5763544.1"
                     /translation="MERHDRDSAGMRYIYAVLSRRAGGVSLGINLNTNNACNWACVYC
                     QVPGLTRGGPEPVDLALLERELSCLLAAIAAGDTAAGIESFGGAQVVDVAFSGNGEPT
                     SAPEFAAALDIVARQLAANRLAVPIRLITNGSLLGRARVRAAIETLGGLQGEVWFKVD
                     RAGSSASRSVNGVARSTKRTIADLLRCAALAPTWVQTCWFANAAGAPDDSEVAAYLAL
                     LAEVRHAVRGVHLYGLARQSMQPGAEHLRRLDAVELEALAKRIRALNLVVTVSP"
     gene            complement(2348..2653)
                     /locus_tag="VIS73_10105"
     CDS             complement(2348..2653)
                     /locus_tag="VIS73_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5763545.1"
                     /translation="MTTTKTPEKIDAREIRKKLGLNQQQFWSRVGVTQSGGSRYESGR
                     NMPRPVRELLRLVHVEQIDLQKIRREDMEVLDYLRSEDPEQLKNLRKLSKAKGRKAA"
     gene            3081..3173
                     /locus_tag="VIS73_10110"
     tRNA            3081..3173
                     /locus_tag="VIS73_10110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3115..3117,aa:Ser,seq:gct)
     gene            complement(3133..3339)
                     /locus_tag="VIS73_10115"
     CDS             complement(3133..3339)
                     /locus_tag="VIS73_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763546.1"
                     /translation="MAKIAIEPKPAAETATQSMPDARGDTDTILGELPNTRRKAPQKK
                     KERTGGALFVKLAETKGFEPLIPF"
     gene            3323..>4115
                     /locus_tag="VIS73_10120"
     CDS             3323..>4115
                     /locus_tag="VIS73_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5763547.1"
                     /translation="MAIFAKIAYAAGVDLQTMLFLRFSIAGIAMLVLMLATRRRWPRG
                     RDLLVLLLLGGVGYVGQSYCFFSSLQHASAGVTALLLYLYPALVALLGWLFLKRPLSM
                     ARIVAIALALAGTAMTVLGALHSTPLGMALGASAALIYAVYILVGEGVTPRVGSVPAS
                     TVIICAAALVYGAAWASGPAELPANGAGWLAVAAIAVLSTLVAILFFFVGLGRLGAAD
                     ASTVSTLEPLATIALAAVFLDERLSAMQIGGGAIIVAAVILIARVR"
BASE COUNT          611 a         1189 c         1433 g          882 t
ORIGIN      
        1 atctgaccca tggcgttggt gcgcgcactg gtcgtcaggt taaccgagat cgtctcgccg
       61 acattcgcgc caacctggaa tgcagcgttg ccgaaagtgc cgtcaagcac tttttggccg
      121 ttaaacgagg tttgagcggc gatccggtcg atttcggcca ggcgctgctg gacttcctgg
      181 tccagcgcct gccggtccga ggcgctgttg gtcgcgttgg ccgactgcac ggccaactcg
      241 cgaatccgct gcaagttgtt gttgatttcg cccagcgcgc cttctgccgt ctgcgccagc
      301 gaaatgccgt cgttggcgtt ccgcgttgcc tgattcaggc cacgaatctg ggtggtgaat
      361 cgctcggaaa tcgccaggcc ggctgcgtcg tccttggcgc tgttgatgcg caggcccgac
      421 gagaggcgct ggagtgcttg cgacagggcg gtctgcgagg tgttgaggtt gcgctgcgag
      481 ttcagcgagg caatgttggt attaattact tgcggcatgg cgatctcctc tagggaattg
      541 atctggttga gttttgaggg cgcgaagttc gatgcctgtc gcgcccttga gtagatttac
      601 gggcgtcgtg cgccgaactt tagggtggct ggagaaatcc tttgggagct cgaagctgcg
      661 ccggcgaacg ccgagcttag gcctgcggag gtgctaagat gcgcgccgct tagctaccca
      721 gcgcggcaac gcaggaggtg tggcagagtg gttgaatgca ccggtcttga aaaccggcga
      781 tggtgaaagc catccgtgag ttcgaatctc accgcctccg ccaagaaaaa acgcgccgtt
      841 tggcgcgttt tgttatttcg atcaaccgct tggatcagtg ctggtcgatc cgtcgggcgt
      901 cggcccagca gttcggcgtc tcgtagaggc ggacctgctc cagttggaga tgattgccgt
      961 agctgtcgcg gtaggcgcgg tcgagcaggt cgaaggcaat ccgcgcgagg ttctcggcgg
     1021 tcggcacgcg gtccagtacc acggtcttgt ggcccgggat cgtggccagg aaggccgtga
     1081 cggcctggtc gccggcatag accagaaagg cgtggtccca ggcgtctaca acctgctggc
     1141 gggcgatgga cttgacctcg gagaagtcca ttaccatgcc gttggccgcg tcaccgtcgc
     1201 gctcgatgat gtcgcccgac agggtgacct cgatcgcgta gcggtggccg tgcaggtgcc
     1261 ggcactggct ggcgtgatcg ggaatgcgat ggccggcgtc gaattcgagg cgtcgcgtga
     1321 tcagcatggt gggcttgggt ggcggtccgg cgcggaaaga gcgcggattc tactgcatgc
     1381 aggcacgagt caaggcagag gagcggcggg caggcgatga gcgcagcgga aaaagttcgg
     1441 aggctgacgg tggaacggca cgaccgcgac agcgccggga tgcgctacat atatgcagtg
     1501 ctgtcgcggc gtgcgggcgg cgtctcgctt ggcatcaacc tcaataccaa caacgcgtgc
     1561 aactgggcct gcgtctattg ccaggtcccg ggtttgactc gcggcgggcc cgagcccgtt
     1621 gatctcgccc tgcttgagcg cgagctgtcg tgcctgctgg ccgcaattgc ggccggcgat
     1681 acggcggccg gtatcgaatc ctttggtggc gcgcaggtcg tcgacgtggc gttttcggga
     1741 aacggcgagc cgacctcggc tcccgagttt gccgcggcgc tcgacatcgt ggcgcggcaa
     1801 ttggccgcaa accggctggc ggttcctatc cgcctgatta ccaacggcag tctgctcggc
     1861 cgcgccaggg ttcgggccgc cattgaaaca ctcggcgggc tgcagggcga ggtttggttc
     1921 aaggtcgatc gggccggctc atcagcttcc cggtcggtga acggggtggc gcgatccacc
     1981 aagcggacga tcgccgacct gttgcgctgc gccgcgctgg cgccgacctg ggtacagacc
     2041 tgctggttcg ccaatgctgc cggcgcgccg gacgacagcg aggtcgctgc ctatctcgcc
     2101 ctcctggccg aggtcaggca tgccgtgcgc ggggtgcacc tgtatggact ggcgcggcag
     2161 tcgatgcagc cgggcgccga gcacctgcgt cgcctcgacg cggtcgaact cgaggcattg
     2221 gccaagcgaa ttcgtgcctt gaatctggtg gttacggtca gtccctgacg ctgggcggag
     2281 agggggctga agcccggcga tgaaaaaggg cgccacggcg cccttgcttg cagactgctc
     2341 ggcgagctca ggcagccttg cggcccttgg ccttggacag cttgcgcagg ttcttcagct
     2401 gttcggggtc ttcgctgcga aggtagtcga gaacttccat gtcctcgcgg cggatctttt
     2461 gcaggtcgat ctgctcaacg tgcaccagac gaagcaactc acgcaccggg cgcggcatgt
     2521 tgcggccgct ttcgtagcgc gaaccaccgc tctgggtgac gccgaccctc gaccagaact
     2581 gctgttgatt caggccgagc ttcttgcgaa tctcgcgtgc gtcgattttc tccggtgtct
     2641 ttgtcgtggt catccgtatt cgccttaagt cgtaatgtcg atgtggtggg atccttggct
     2701 gcctacccga aagccataca gggctggtat cactaaggtg ataggcatta taggtatcga
     2761 tcataaatat gcaagctgcg atacggaact atttggcgtt tttttgcgcc ggccgtcgat
     2821 cgcggcagca ttccgactgg gggttagcat ggaccggttg gatcgagtgg cgattatttc
     2881 gttgggatgg ctggcgaacg gtatgcgggg gcggtggcca ataccggcat cgatgcggac
     2941 gcgatcttcc gggcgattga aatggatcgc atcagttccg cggcttggca tttcgggagc
     3001 agggggcgtc agagccggtg ggaacgccct gtcgtcgttt gaccgtccgc gggctagcgg
     3061 ctatagtctc gcccgctttc ggagacgtgg ccgagtggtc gaaggcactc ccctgctaag
     3121 ggagcatacc ccttaaaagg gtatcaaggg ttcgaatccc ttcgtctccg ccagctttac
     3181 gaagagcgcg ccgccggtgc gctctttctt tttctgcggc gccttgcgcc gggtattggg
     3241 taactcgccc aggatggtgt cggtgtcccc tcgcgcgtcg ggcatgcttt gggttgcggt
     3301 ttcggcggcc ggttttggct cgatggcgat ctttgccaag atcgcctacg cggccggtgt
     3361 tgatctgcag acaatgctct tcctgcgctt ctcgatcgca ggtatcgcga tgctggtcct
     3421 catgctggcg acgcggcgcc gctggccgcg cggccgcgat ctgcttgttc tgttgctgct
     3481 cggcggtgtc ggctatgtcg gccagtccta ctgcttcttt tcgtcgctcc agcacgcgag
     3541 cgccggcgta accgccctgt tgctctacct gtatccggcg ctggtggcgc tgctcggctg
     3601 gctgttcctg aagcgcccgc tgtcgatggc gcgaatcgtg gcgattgcgc tggcgctcgc
     3661 ggggactgcg atgaccgttc ttggcgcgct gcacagcacg ccgctcggca tggcgctggg
     3721 ggcgtcagca gcgctgatct acgcggtata catactggtc ggcgagggtg tgacgccgcg
     3781 cgtcggcagc gttccggcat cgacggtcat catttgtgcg gccgcgctcg tctatggcgc
     3841 agcctgggcc agcggccctg ccgagttgcc ggcgaatggc gctgggtggt tggccgtggc
     3901 ggcaatcgcg gtcctgtcga cgctggtggc gatcctgttt ttctttgtcg ggcttggtcg
     3961 gcttggggcg gccgatgcct ccacggtctc gacgctcgaa ccgctggcaa cgatcgcact
     4021 ggcggcggtg tttctcgacg agcgtctgtc ggcgatgcag atcggcggcg gcgcgatcat
     4081 tgtcgcggcg gtgatcctga ttgcgcgggt gcgtg
//
LOCUS       DATDAA010000331         3065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_268756,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000331 DATDAA010000000
VERSION     DATDAA010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268756"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VIS73_10125"
     CDS             complement(<1..358)
                     /locus_tag="VIS73_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005173136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY5763548.1"
                     /translation="MLIIPAIDLKDGHCVRLKQGLMDDATVFSEDPGEMAATWLAQGA
                     RRLHIVDLNGAFAGKPKNERAIKSIVAALGDEIPVQLGGGIRDLDTIEAYLDDGITYV
                     IIGTAAVKNPGFLHDAC"
     gene            complement(414..1052)
                     /gene="hisH"
                     /locus_tag="VIS73_10130"
     CDS             complement(414..1052)
                     /gene="hisH"
                     /locus_tag="VIS73_10130"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY5763549.1"
                     /translation="MARIVVVDSGMGNLRSVSQAIAHVAGADAVTISDSPEAVRRAER
                     VVFPGQGAMPDCTRELEARGLHQAVVEAAGEKPFLGICIGEQMLFDWSDEGGVPGLGV
                     FAGRVERFPEAAMHDASGARLKVPHMGWNGVRQRQRHALWRSIDDGSRFYFVHSYFVV
                     PADQSIIAGESTHGVAFTAAVARDNIFAVQFHPEKSATAGLQMLTNFVGWKP"
     gene            complement(1089..1676)
                     /gene="hisB"
                     /locus_tag="VIS73_10135"
     CDS             complement(1089..1676)
                     /gene="hisB"
                     /locus_tag="VIS73_10135"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY5763550.1"
                     /translation="MRQADIQRNTQETRIRVRIDLDGSGRGQFKTGVPFLDHMLDQIA
                     RHGMIDLEVEAEGDLHIDAHHTVEDIGITLGQAVAKALGDKRGVTRYGHAYVPLDEAL
                     SRVVVDLSGRPGLSFNVAFTRAWIGQFDVDLIREFFQGLVNHAALTLHIDNLRGDNAH
                     HQAETVFKAFGRALRMAATVDPRAAGTIASTKGSL"
     gene            1789..2847
                     /gene="hisC"
                     /locus_tag="VIS73_10140"
     CDS             1789..2847
                     /gene="hisC"
                     /locus_tag="VIS73_10140"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY5763551.1"
                     /translation="MSRYWSDTVRRLTPYVPGEQPKLDRLVKLNTNENPYGPSPRALA
                     AMRAASDDSLRLYPDPNADLLKATIAHRFGIAANEVFVGNGSDEVLAHVFQALLKHER
                     ALRFPDITYSFYPVYAGLYGIAVETVPVDADYGVRVAGYAGPCGGIVIPNPNAPTGVA
                     LPLAEIETLVASHPECVVVIDEAYVDFGAESAVPLIRRFPNLLVVQTLSKSRSLAGLR
                     VGFALGHAELIDALERVKNSFNSYPLGRLAQIGAIAAIEDEAWFDETRGKVIASRQRL
                     TAELTTLGFVVLPSQANFVFASHRSRRADELAAALRERKVIVRHFKAARIDNHLRITI
                     GDDGQCDALLAALREILA"
     gene            complement(2844..>3065)
                     /locus_tag="VIS73_10145"
     CDS             complement(2844..>3065)
                     /locus_tag="VIS73_10145"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY5763552.1"
                     /translation="SLGDYCAGPNHVLPTSGSARFSSPLGVYDFQKRSSLIRVSAVGA
                     QTLGRIASTLAWGEGLPAHARSAEFRLDP"
BASE COUNT          514 a         1015 c          998 g          538 t
ORIGIN      
        1 cgcaggcatc gtgcaggaaa ccggggttct tcaccgcggc ggtgccgatg atgacgtagg
       61 tgatcccgtc gtcgaggtag gcctcgatgg tgtcgaggtc gcggatgccg ccacccagct
      121 gaaccggaat ctcgtcgcca agcgcggcta cgatcgactt gatcgcccgc tcgtttttcg
      181 gtttgccggc gaaggcgccg ttgaggtcga cgatgtgcag gcgccgcgcc ccctgcgcca
      241 gccaggtcgc tgccatctcg cccggatcct cggagaagac ggtcgcgtcg tccatgaggc
      301 cttgcttcag gcggacgcag tggccgtcct tcaagtcgat tgcgggaatg atcagcatga
      361 tgggttcggg acgtcgagtc ggaagaaaat ggcttgtcag caggtgcgcg tagtcagggt
      421 ttccagccga cgaagttcgt cagcatctgc aatcccgccg tcgcgctctt ctcaggatgg
      481 aactgaacgg cgaaaatatt atccctcgcc accgccgcgg taaaggccac gccgtgcgtg
      541 gattcgccgg cgatgatcga ttggtcggcg ggaaccacga agtaactgtg cacgaaatag
      601 aagcgcgatc catcatcgat cgagcgccac agtgcatgcc gctggcgctg gcggacgccg
      661 ttccagccca tgtgcgggac cttcagccgg gcgcccgagg catcgtgcat cgccgcctcg
      721 gggaagcgct cgacccgtcc ggcgaagacg ccgagccctg gcacgccgcc ttcgtcgctc
      781 cagtcgaaga gcatctgttc gccgatgcag atgccgagaa atggcttctc gcctgcggcc
      841 tcgaccacgg cctgatggag gcctcgcgcc tcgagttcgc gcgtgcagtc aggcatggcc
      901 ccctgaccgg gaaagaccac gcgctcggcg cggcggaccg cctcgggcga atcgctgatc
      961 gtgactgcgt cggctccggc gacatgcgca atcgcctgcg ataccgagcg cagattgccc
     1021 atgccggagt cgacgacgac gatgcgtgcc atgttcgatt caaccctctt cggccagccc
     1081 gatagttcct acaggctgcc tttcgtcgag gcgatggtgc cggcagcgcg cggatcgacg
     1141 gtcgccgcca tgcgcaatgc ccgcccgaag gccttgaaca cggtctcggc ctgatgatgg
     1201 gcgttgtcgc cgcgcaggtt gtcgatgtgc aaggtcagcg ccgcgtgatt gaccagcccc
     1261 tggaaaaact cgcgaatcag gtcgacgtca aactggccga tccaggcgcg cgtgaaggcg
     1321 acgttgaagg acagccccgg gcggcccgac aggtcgacga cgacgcgcga gagcgcctcg
     1381 tcgagcggca cgtaggcgtg cccgtagcgg gtgacgccgc gcttgtcgcc gagggccttg
     1441 gcgactgcct ggccgagcgt gatgccgata tcctcgaccg tgtgatgcgc gtcgatgtgc
     1501 aggtcgccct ctgcctcgac ctcgaggtcg atcatgccgt ggcgtgcaat ctggtcgagc
     1561 atgtggtcga gaaaggggac gccggtcttg aactggccgc ggccactgcc gtcgagatcg
     1621 atgcggaccc ggattcgggt ctcctgcgtg ttgcgctgaa tgtcggcttg ccgcatggtg
     1681 tgggcgctga agcgttggtt cgggcggcca tgatagcatt caaaagccgc atgcaaggcg
     1741 gccttcagcc ccgccaatca acgctttcgg accgtttccc caccgcccat gagccgctac
     1801 tggagtgaca ccgttcgccg cctgacgccc tatgttccgg gcgagcaacc caagctcgac
     1861 cggctggtca agctcaatac caacgagaac ccctacggcc cctcgccgcg cgcactggcc
     1921 gcgatgcgtg ccgccagcga cgattcgctg cgcctctacc ccgatcccaa cgccgatttg
     1981 ctgaaggcga cgatcgccca ccgcttcggg atcgctgcca acgaagtctt cgtcggcaac
     2041 ggttccgatg aggtgctggc ccacgtcttt caggctttgc tcaagcatga gcgagcactt
     2101 cgcttccccg acatcaccta cagcttctac ccggtttatg cggggctcta cggcattgcc
     2161 gtcgaaacgg ttccggtcga cgccgattat ggtgtgcgcg tcgccggcta cgccgggccc
     2221 tgcggcggca tcgtcatccc gaacccgaac gcgccgacgg gcgtcgccct gccgctggcg
     2281 gaaatcgaga cgctggtggc atcgcacccc gaatgcgtgg tggtgatcga cgaagcctac
     2341 gtcgacttcg gcgccgaatc ggcggtgccg ctgatccgcc gctttcccaa tctgctggta
     2401 gtgcagacgc tgtcgaagtc gcgttcgctg gccggtctgc gcgtcggctt cgccctcggg
     2461 cacgccgagc tgatcgacgc gctggaacgc gtcaaaaaca gcttcaattc gtatccgctc
     2521 ggccgtctcg cgcaaatcgg cgcgattgcg gcgatcgaag acgaggcctg gttcgacgag
     2581 acgcgcggca aggtcatcgc cagccgccag cgcctgacgg ccgagctgac gacgctcggt
     2641 tttgtggtgc tgccctcgca ggccaatttc gtcttcgcca gccaccgctc acggcgggcc
     2701 gacgaactgg ccgccgcgct gcgcgaacgc aaggtgatcg tccgccattt caaggcggca
     2761 cgaatcgaca accacctgcg cattaccatc ggcgacgatg gccaatgcga cgcgctcttg
     2821 gcggcgctgc gcgaaatcct cgcttagggg tctaggcgaa actcggccga acgggcgtgc
     2881 gccggcaggc cttcgcccca tgccagcgtc gaggcgatcc gccccagcgt ctgcgcgccg
     2941 accgccgata cgcggatcag gctcgagcgt ttctggaagt cgtagacgcc aagtggcgat
     3001 gagaaccgcg cgctgcccga ggtcggcagc acgtggttgg gtccggcgca gtaatcgccc
     3061 agcga
//
LOCUS       DATDAA010000332         2957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_234483,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000332 DATDAA010000000
VERSION     DATDAA010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234483"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..413)
                     /locus_tag="VIS73_10150"
     CDS             complement(39..413)
                     /locus_tag="VIS73_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763553.1"
                     /translation="MTCRPVLVFVASILLALPAAAQIAKSGAGTIDDIQITGDRDVPM
                     AMVVLPWLEPAVAQPPAYFQSGLPRVFEHDRSLAHDPLNRSVAVLQMAKQEAVEQAQV
                     LEAEREAASKKRRRRSRDGDAD"
     gene            complement(415..>2957)
                     /locus_tag="VIS73_10155"
     CDS             complement(415..>2957)
                     /locus_tag="VIS73_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5763554.1"
                     /translation="REAGIHYESLLKDYADLTDGADVRYQLARIQDITGQPEKVQESL
                     TAFVRQYPQDPRIADATFRLAELRFGFGDYEGARVAYEGVLKRGDERYRDQAEYKLAW
                     SLFKQGEHRSALPRFLAVIDRAADPAKGSDRLTRERMKDAFRAAALTYSYLDGPADIE
                     RSFARGPKKAYVADLYASLAQLYIDRDRINDAARTWEALNKHYPDDSRAPELLAGVVQ
                     GAREEGLSKIALDMQEQYIVRYAKGSDYWNRAEPDARAAIDARMQPMLAEMAKMYHAD
                     AQQQKNAVSRQKAIRYYDQYVTTFPADKDTPGYRFLLAEARYENGELAAALTDYEAAA
                     YQYGPHAKAAEAGYATLVASQQLVERARGDDARKRELRALATRSDRFAQGFPQDLRVE
                     AVLAKAGEDLLLLGESAEAAKIGNALIARKPEANIRRRAVLILAHGQFESGGFAEAEK
                     AYAEALSLRPDAKQAKELTDRLGLAVYRQAEAARAAGQATEAIAGFLRVAKTAAGAEA
                     VPNAEIDAAALLLETKRWGEAIDVLERFGKNYPGHQLGTDVPTRLAYAYENDRQYLKA
                     AELLETISQTEKDDALARQTLWRSAELRDKGGRRDLTLATWERYLKRYPAPLEKASEV
                     RQLLADAAAKSGDKPTRNKWLNEIIATHGKGGAEATVRVAYLAAQAHLVYGDEAGAEF
                     DVLKLKLPLDKSLAAKRKALEQSLKWYDGAGRYGIAEVTTNATYKTADLYARLAKDLM
                     SSDRPSGLTALEKEQYDILLEEEAFPFEDKATQIHELNHQRLKDGIYDGWVKQSMGAL
                     ATLVAAKYDRAIPEISFFSYEPPRPEPVKAAPVGTPAQRKEGGNAKEG"
BASE COUNT          407 a          926 c         1035 g          589 t
ORIGIN      
        1 tctacggcag cacgcggcac tgatcccgcc atccctactc agtccgcatc cccgtcacgc
       61 gatcggcgcc ttcgcttctt cgaagccgcc tctctttccg cctcgagcac ctgcgcctgt
      121 tcgacggcct cctgtttggc catctgcagg acagcgactg accggttgag cggatcatgc
      181 gccagactgc gatcgtgttc gaagacccgt ggtagcccgc tttgaaagta ggcaggcggt
      241 tgcgcaactg ccggttccag ccagggcagc acgaccatcg ccatcggcac gtcgcggtcg
      301 ccggtaatct gaatgtcatc aatcgtgcct gccccggatt tcgcgatctg ggccgccgcc
      361 ggcagagcga gcaagatcga ggcaacgaag accagcaccg ggcggcaggt caattcaacc
      421 ctccttggca ttgccgccct ccttgcgctg cgcgggagtc ccgactggcg ccgccttgac
      481 cggttctggc cttggcggct cataggagaa gaaggatatc tccgggatgg cacgatcgta
      541 cttggcggcg accagcgtcg ccagcgcgcc catgctctgc ttcacccagc catcgtagat
      601 accgtccttc agccgctggt gattgagttc gtggatctgc gttgccttgt cttcaaacgg
      661 gaaggcctct tcctcgagca ggatgtcgta ctgctccttc tccagcgcgg tcagccccga
      721 gggccggtcg gaactcatca ggtccttggc cagccgggcg tagaggtcgg cggtcttgta
      781 ggtggcgttg gtggtgactt cggcgatgcc gtagcggccg gcgccgtcgt accacttgag
      841 gctctgctcg agcgccttgc gcttggcagc gagcgacttg tcgagcggca gcttgagttt
      901 gagcacatcg aactcggcgc cggcttcgtc gccgtagacg aggtgtgcct gggcggcgag
      961 gtaggcgaca cggacggtag cctcggctcc gcccttccca tgcgtggcga tgatttcgtt
     1021 gagccacttg ttgcgggtgg gcttgtcgcc agacttggct gcggcgtcgg ccaatagctg
     1081 ccgcacttcg ctggccttct cgagcggcgc cgggtagcgc ttgaggtagc gctcccaggt
     1141 ggcgagcgtg aggtcgcgcc ggccgccctt gtcgcgcagt tcggcagagc gccacagcgt
     1201 ctgccgggcc agcgcgtcgt ctttctcggt ctgcgagatg gtttcgagca gctcggcggc
     1261 cttgaggtac tggcgatcgt tctcgtaggc gtaggcgagc cgcgtcggca cgtcggtacc
     1321 gagctggtgg ccggggtagt tcttgccgaa gcgctcgagg acgtcgatcg cctcacccca
     1381 gcgcttggtt tccagcagca gggcggcagc gtcgatctcg gcgttgggca cggcctcggc
     1441 cccggcggcg gtcttggcga cgcgcaggaa gccggcgatg gcctcggtgg cctggccggc
     1501 ggcgcgcgcg gcttcggcct ggcggtagac ggcaagaccc agccggtcgg tgagttcctt
     1561 ggcctgtttc gcatcgggcc gcaggctgag cgcctcggcg taggccttct cggcttcggc
     1621 aaagccgccg gactcgaact ggccgtgggc gaggatgagg acggcgcgac ggcggatgtt
     1681 agcttcaggt tttctggcga tcagggcgtt gccgatcttc gcggcttcgg cggattcacc
     1741 caggagcagc aggtcttcac cggccttggc gaggacggct tcgacgcgca ggtcctgcgg
     1801 gaagccctgg gcgaaacggt cgctgcgcgt cgcaagcgct cgcagttcac gcttcctggc
     1861 gtcgtcaccc ctggcccgct cgaccagttg ctggctggcc accagcgtgg cgtagccggc
     1921 ctcggcggcc ttggcgtgcg ggccgtactg gtaggcggca gcttcgtagt cggtgagcgc
     1981 cgcggccaac tcgccgttct cgtagcgagc ctcggccaac aagaagcggt agcccggggt
     2041 gtccttgtcg gcggggaagg tggtgacgta ctggtcgtag tagcggatgg ccttttgccg
     2101 ggagacggcg ttcttctgct gctgggcgtc ggcgtggtac atcttggcca tttcggcgag
     2161 catgggctgc atgcgggcgt cgatggcggc ccgcgcgtcg ggttcggcgc ggttccagta
     2221 gtcgctgcct ttggcgtagc ggacgatgta ctgctcctgc atgtcaagcg cgatcttgct
     2281 caggccttct tcgcgcgcgc cctggacgac gccggcaagc agttcggggg cgcggctgtc
     2341 gtcggggtag tgcttgttca gtgcttccca ggtgcgggcg gcgtcgttga tgcggtcgcg
     2401 atcgatatag agctgcgcca gactggcgta gagatcggcg acgtaggctt tcttcgggcc
     2461 gcgggcgaag ctccgttcga tgtcggcggg gccatccaga tacgagtagg tgagcgcggc
     2521 ggcgcggaag gcgtctttca tgcgctcgcg cgtgagccgg tcgctgccct tggcggggtc
     2581 ggcggcacgg tcgatgacgg cgaggaagcg cggcagcgca ctccggtgtt cgccttgctt
     2641 gaagagactc caggcgagct tgtattccgc ttgatcccgg taacgttcgt ccccacgctt
     2701 caagaccccc tcgtacgcca ccctcgcccc ttcgtaatca ccaaatccaa agcggagttc
     2761 ggcgagacgg aaggtcgcat cggcgattcg cgggtcctgc ggatactggc ggacgaaggc
     2821 ggtgagcgac tcctgaacct tttccggctg cccggtgatg tcctgaattc gcgccagttg
     2881 gtagcgaacg tcggcgccat cggttaggtc agcgtagtcc ttgagcaagc tctcgtagtg
     2941 gatgccggcc tcacgcg
//
LOCUS       DATDAA010000333         2134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_199391,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000333 DATDAA010000000
VERSION     DATDAA010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199391"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VIS73_10160"
     CDS             complement(<1..880)
                     /locus_tag="VIS73_10160"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY5763555.1"
                     /translation="MSGYPLLSLAIWIPILAGLVVLATGSDRNAPLARIIALFGAVVG
                     FLVTIPLYTGFDLTTADMQFVELASWIPRFNVNYYLGVDGISVLFVLLNSFITILVVL
                     AGWKVIQNKVSQYMGAFLIMSGLMNGIFSALDAVLFYVFFETSLIPMYIIIGVWGGQN
                     RVYAAIKFFLYTLMGSLLLLVAFLYLFLESGGSFNILDWHRTPLTMTAQILCFLAFLV
                     AFGVKVPMWPVHTWLPDAHVEAPTGGSVVLAAIQLKLGAYGFIRFAMPITPDGAHELA
                     WLMITLSLIAVVYIGFV"
     gene            complement(903..>2134)
                     /locus_tag="VIS73_10165"
     CDS             complement(903..>2134)
                     /locus_tag="VIS73_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY5763556.1"
                     /translation="LFELSTTALGFVIVIGAITALFMGFLGIIQNDIKRVVAYSTLSQ
                     LGYMTVALGASAYSVAIFHLMTHAFFKALLFLAAGSVIIAMHHEQDIRNMGGLRKYMP
                     ITWITSLIGSLALIGTPFFAGFYSKDLIIESAKFATVPGATFAYYCVLAGVFITAFYS
                     FRMYFLVFHGEERFGKPHAAHDDHHAAEAHGDDAHADAHGDDDHSHDDHHHGLAPGEK
                     PHETPWVVWAPLVALAIPSVIIGYIGVEPMLFGGYFDNVIAVDATAHPAMAMIAEHFH
                     GAMALGLHAFQTLPFWLAAAGVGLSWFFYMKRPDIPAAIQVKFSGLYKLLDNKYYFDR
                     FNEIFFAGGARGLGKGLWRGGDVGVIDGVLVNGSAKLVGWIASVVRHFQSGYIYHYAF
                     TMLIGLFVLMTLWVNRG"
BASE COUNT          513 a          612 c          682 g          327 t
ORIGIN      
        1 ccacaaagcc gatgtagacc accgcaatca gcgacagcgt gatcatcagc caggccaact
       61 cgtgtgcgcc gtccggagtg atcggcatcg cgaaccggat gaaaccgtag gcgccgagct
      121 tgagctgaat ggctgccagc accaccgaac caccagtcgg cgcctcaacg tgcgcatcgg
      181 gcaaccaggt gtgcaccggc cacatcggca ccttgacgcc aaaggcaacg aggaaggcga
      241 ggaagcagag aatctgcgcc gtcatggtca gcggcgtgcg gtgccaatcg aggatgttga
      301 agctgccgcc cgactcgaga aacagataaa gaaaggccac cagcagcagc agcgagccca
      361 tcagggtgta gaggaaaaac ttgatcgccg cgtagacgcg gttctggccg ccccagacac
      421 cgatgatgat gtacatcgga atcagcgagg tctcgaagaa aacgtagaac agcacggcat
      481 cgagtgccga gaagatgccg ttcatcaggc ccgacatgat caggaaggcg cccatgtact
      541 gcgacacctt gttctggatc accttccagc cggcgagcac gacgagaatc gtgatgaagc
      601 tgttgagcag gacgaagagc accgagatgc catcgacgcc aaggtagtaa ttgacgttga
      661 agcgcggaat ccagctggcg agttcgacga actgcatgtc ggcagtcgtc agatcgaagc
      721 cggtatagag cgggatcgtg acgaggaaac caaccaccgc accaaacaac gcaatgattc
      781 gcgccagcgg cgcgttgcgg tcggagccgg tggcgagtac caccaggccc gcaagaatcg
      841 ggatccagat ggcgagactc agcaaggggt agccagacat attgttttcc gaaaaacgtc
      901 gtttatccgc ggttgaccca gagcgtcatc aggacgaaca ggccgatcag catcgtgaag
      961 gcgtagtggt agatgtagcc ggactggaag tggcggacca ctgaagcgat ccagccaacc
     1021 agcttcgccg aaccgttgac gagaacaccg tcgatgacgc cgacgtcgcc accgcgccag
     1081 aggcccttgc ccaagccgcg cgcaccgcca gcaaagaaga tttcgttgaa gcggtcaaag
     1141 taatacttgt tgtcgagcag cttgtagagg ccgctgaact tcacctgaat cgccgccggg
     1201 atatcgggac gcttcatgta gaaaaaccag gacagaccca cgccagcagc tgccagccag
     1261 aacggcagtg tctggaaggc gtgcaggccc agcgccatgg ccccgtggaa gtgctcggcg
     1321 atcattgcca tcgccgggtg cgccgttgca tcgacagcaa tcacgttgtc gaagtagccg
     1381 ccgaagagca taggctcgac gccgatgtag ccgatgatga ccgaggggat cgccagcgcg
     1441 accagcggcg cccagaccac ccaaggcgtc tcatgcggct tctcgccggg cgccaggccg
     1501 tgatgatggt cgtcgtggct gtgatcatcg tcgccatgtg cgtctgcatg ggcgtcgtcg
     1561 ccatgcgcct cggccgcgtg atggtcgtcg tgcgcggcgt gcggcttgcc gaaacgctcc
     1621 tcgccgtgga agaccaggaa gtacatgcgg aaggagtaga aggccgtgat gaagacgccg
     1681 gccagtacgc agtagtaggc aaaggtcgca ccggggacgg tggcgaactt cgccgactcg
     1741 atgatcaggt ccttcgaata gaagccagcg aagaacggcg taccgatcag tgcaagcgaa
     1801 ccgattagcg aggtgatcca ggtgatcggc atgtacttgc gcaggccacc catgttgcgg
     1861 atgtcctgct cgtgatgcat ggcgatgatg accgaaccgg ccgcgaggaa cagcagcgcc
     1921 ttgaagaagg cgtgggtcat caggtgaaag atcgccaccg agtaggccga agcaccgagc
     1981 gcaaccgtca tgtagccgag ctgcgagagc gtcgagtagg cgacgacacg cttgatgtcg
     2041 ttctggatga tgccgaggaa gcccatgaac agggccgtga tcgcgccgat gacgataacg
     2101 aacccgagcg ccgtggtcga cagctcgaac agcg
//
LOCUS       DATDAA010000334         2107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_94760,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000334 DATDAA010000000
VERSION     DATDAA010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94760"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            519..>2107
                     /locus_tag="VIS73_10170"
     CDS             519..>2107
                     /locus_tag="VIS73_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5763557.1"
                     /translation="MNADLLQGFYLGDLLVDPATGRVTGRSGSSHLPSKAVEVLLCLA
                     SSPGQLVSRRKLIATVWGEGRGSDEALSHAVSEIRHALGDHHEQPVFIQTLPKRGYRL
                     LLDPDPVIAGNSTIIKNGGPVRDLSLLDNLVQRGVIETGVAYLIFGWLLIQVADIVFD
                     QLHFPDWAGTFVTLLVIAGFPIALLLSWFLEIRGGNAVLDRVTPKNARRRRVSRTYVS
                     VISAFAIAAVAVFAYDRTVGLPQARPVADAQPGPEVVLPPIEEHSLAVLPFLNLDGSD
                     ETAVFANGLVDDVLTSLARVPGLLVASRGDAYSLEPNSPSKKVRERLRVARYVEGSVQ
                     ITDDRIRVIVQLIDSETGFHVMSRSFDRPREDFFDIRDEVTSLTVANMRPALPPKTRM
                     AEVQTVDDPALDVYMLYRRGVDASRRPTTAETITESLDWFDRALAIDPDYAAAHAGKC
                     NTYAIGYPETDDAEFIAKAEASCARALALNPNLDVVHTSLGLLYVSTGRYDAAIEAYS
                     RALDTDPSNVDALLGLGDAYR"
BASE COUNT          374 a          719 c          634 g          380 t
ORIGIN      
        1 gtcggaccgg cacgccgcgc gagcaatatc acgcccgcga tcgcgacgcg ctcgagctga
       61 acgcttgttc gccctgtgcg ggcccggcaa taacgggtcc gcgcagggcg cgtggcgtta
      121 tctgcgcccg cgcttcactg ctgtgcgcgc atcactgtaa cccccccgct ggctgaccgt
      181 tccggcgttt tccgcagcga cgctcgccgg atcaagaagt cacgaactcc attttaggaa
      241 aagaagcaac tgcatgggtg tagcgcccgc cgctccagac cgctaccccc atcgcctgat
      301 cgcgagatcg agttccctca acgaagttcc cgcacgggtt gagcgccggc cgcttagcgg
      361 aatcccgcga tcacttgtct accagattga ttccagtcga cgagactctg gtacggaggc
      421 gatggcgggc actctgcgcc gacggcgcga gcagcgtata attacgccga ctgtacaact
      481 gtcgtccgtc ccggaaggca gtcgatgggg gcagtcgcat gaatgccgat ctcctgcagg
      541 ggttctacct cggggacctg ctcgtcgatc ccgccaccgg gcgggtgacc ggccgatcgg
      601 gttcctcaca ccttccatcg aaggcggtgg aagtcctgtt gtgtctcgcc tcctcacccg
      661 gccagctcgt ctcgcggcgc aagctgatcg cgaccgtctg gggcgaaggc cgcggcagcg
      721 acgaggcgct gagccatgcc gtcagcgaaa ttcgtcacgc gctcggcgac caccacgagc
      781 agcccgtctt catccagaca ttgccgaaac gcggctaccg gctgttactc gatccggatc
      841 ccgtcattgc aggaaactcg acgatcatca agaacggcgg tcctgttcgt gacttgagcc
      901 tgctcgacaa tctcgtgcag cgcggcgtca tcgagacggg cgtggcctac ctgatcttcg
      961 gctggctgct gatccaggtc gccgatatcg tcttcgacca gctgcacttc ccggactggg
     1021 ccggtacgtt cgtgacgctg ctcgtgatcg cgggattccc gatcgcgtta ctgctctcct
     1081 ggtttctcga gattcgcggc ggcaatgccg tgctcgaccg cgtcacgccg aagaacgccc
     1141 ggcgcagacg ggtgagccgg acctatgtct ccgtgatcag cgccttcgcg atcgccgcgg
     1201 tcgccgtgtt cgcctatgac aggaccgtcg gattgccgca ggctcgccca gtcgcggacg
     1261 cgcagcccgg acccgaagtc gtgctgccgc cgatcgaaga gcactcactc gccgtactcc
     1321 ccttcctgaa cctcgacggc agcgacgaga ccgcggtgtt cgccaacgga ctcgtcgacg
     1381 acgtgctgac aagcctcgca cgcgtcccgg ggctgctggt cgcatcgcgc ggcgacgcct
     1441 actcactcga acccaattcg ccctcgaaaa aagtgcgcga gcggctccgc gttgcacgct
     1501 acgtcgaggg cagcgtacag atcacagacg atcgcatccg cgtgatcgtg cagctgatcg
     1561 attcggagac cggtttccac gtcatgtcgc gcagcttcga tcgcccgcgc gaggacttct
     1621 tcgatattcg cgacgaagtc acgagtctca cggtcgcaaa catgcgcccc gcactgccgc
     1681 cgaagacacg catggccgaa gttcagaccg ttgacgatcc ggcgctggac gtctacatgc
     1741 tctaccgccg cggtgtcgat gcgtcgcgga ggccgacgac ggccgaaacc atcaccgagt
     1801 cgctggactg gttcgacagg gcgctcgcga tcgatcccga ctacgccgct gcgcacgccg
     1861 gcaaatgcaa tacctacgcg atcggttacc cggaaaccga cgacgcagaa tttattgcaa
     1921 aggccgaggc atcctgcgcg cgggcgctgg cactgaatcc taacctcgac gtcgtccaca
     1981 catcactcgg cctgctatac gtatcgacgg ggcgctacga tgccgctatc gaagcgtact
     2041 cgcgggcact ggacacggac ccgtcaaatg tcgatgcgct gctcggtctc ggtgacgcct
     2101 accggat
//
LOCUS       DATDAA010000335         2550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_199612,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000335 DATDAA010000000
VERSION     DATDAA010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199612"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..495)
                     /locus_tag="VIS73_10175"
     CDS             complement(109..495)
                     /locus_tag="VIS73_10175"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEY5763558.1"
                     /translation="MRHKFLGVGEPGYHPIRKLRTVVSGLRYAVLYDWSVTYKLVLSL
                     VVLCFAFAYREWVDFLVVLVATAMVLTAEILNSAIEALCDFVEDRHNEKIKIIKDISA
                     AGVGISILVWMIVLVVEVTQVPGIGR"
     gene            complement(492..1109)
                     /locus_tag="VIS73_10180"
     CDS             complement(492..1109)
                     /locus_tag="VIS73_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5763559.1"
                     /translation="MRQSLTTLAAVALTGSLVGAIGLAVTGQVPAIDAAGLALADNVR
                     RPALDAAFGTITWLGSIVVLLPLALVVALRVAPRLGWRAATLAPAVLLATTALAQVGK
                     LVFERARPDLYPALTTMPADASFPSAHAAQAAAVALALLMHSSLAGRQRAVLAAGLGF
                     LVGAVALSRIYLQVHFPSDVVAGLLLAVAVVIGLRALPFWRGAKL"
     gene            complement(1106..1324)
                     /locus_tag="VIS73_10185"
     CDS             complement(1106..1324)
                     /locus_tag="VIS73_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763560.1"
                     /translation="MDTIARVALANRQSPQAIGNLFRSSLRPVADARVDVPEVQESTR
                     VSFSKGAAVAASRPASSAVALYRWVQSL"
     gene            complement(1337..2221)
                     /gene="htpX"
                     /locus_tag="VIS73_10190"
     CDS             complement(1337..2221)
                     /gene="htpX"
                     /locus_tag="VIS73_10190"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEY5763561.1"
                     /translation="MNLKRVGLFLMTNLAVLVTLSVVAHLFGFNQFVSAAGLDFGALL
                     GFAALFGFGGAFISLAMSKWVAMKSVGARVIEAPANDLELWLVATVRRQAQAAGIGMP
                     DVAIYDADDVNAFATGMKRNHALVAVSTGLLRKMTRDEAEAVLAHEVSHIANGDMVTL
                     ALIQGVVNTFVIVIARAVGYFVDRVLLKNEGGLGVGYYATFIIMEIGLGILASLLVMH
                     FSRQREFRADAGGASIAGRSRMIAALRRLKASHEPSALPDRVAAFGISGNRAGGWRAL
                     FTTHPPLEARIAALEAAN"
     gene            complement(2305..>2550)
                     /locus_tag="VIS73_10195"
     CDS             complement(2305..>2550)
                     /locus_tag="VIS73_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEY5763562.1"
                     /translation="EVYPWAWAFFLVFILASTFTLLNLFIAVIVNAIQHENQAAAAAE
                     EPPPNDSQVAALRAEIRALRAELRQSTPTRHRRRRGT"
BASE COUNT          518 a          821 c          857 g          354 t
ORIGIN      
        1 aaattctagg agaacgactt ctggaaatcc ccggggcgcc gacatttccg ggacgtcacg
       61 agtcgcgcgc accacgtccc gatgctcgcg ggttggcgaa agaatggatc agcggccgat
      121 ccccggaacc tgcgtaacct cgacgaccag cacgatcatc cagacgagga tactgatgcc
      181 gacaccggct gcggagatgt ccttgatgat cttgatcttc tcgttgtgac ggtcttcgac
      241 gaaatcgcaa agtgcttcga tcgcgctgtt aagtatctcc gctgtcagca ccatggcggt
      301 cgcgacgagg accacgagaa agtcgaccca ctcacggtag gcgaaggcga agcagagcac
      361 caccagcgac agcaccagct tgtaggtgac cgaccagtca tagagcacgg cgtaacgcag
      421 gccggaaacc accgtgcgca acttacgaat cgggtgatag ccgggttcgc cgacaccgag
      481 aaacttgtgt ctcacagctt ggctccccgc cagaacggca gcgcgcgcag gccgatcacg
      541 acggccacgg caagcaacag gccggcgacg acgtcgctgg gaaagtgcac ctgcaggtaa
      601 atccgcgaca gcgcgacagc gccgacgagg aagccgagac cggcggccaa caccgcgcgc
      661 tggcgaccgg cgaggctcga atgcatgagc agcgccaacg ccacggccgc cgcctgcgcc
      721 gcgtgcgcgc tgggaaagct ggcatcagcg ggcatcgtcg tcagcgccgg atagaggtcc
      781 gggcgggcgc gctcgaagac gagcttgccc acctgcgcca gcgcagtggt cgcgagcagc
      841 acggccggcg ccagggtggc ggcacgccag ccaagacgcg gcgccacgcg cagcgcgacg
      901 acgagcgcca agggcagcag cacgacgatc gagccaagcc aggtgatcgt cccgaaggcc
      961 gcatccaggg ccggccggcg cacattgtcg gccagcgcca gcccggccgc gtcgatggcc
     1021 ggaacctggc cggtgacggc aaggccgatg gcgccgacga gactgccggt cagcgcgacg
     1081 gccgctagcg ttgtcaggga ttgcctcaca aggactgcac ccagcgatag agggcgacgg
     1141 ccgaactcgc tggtcgactc gctgccacgg cggcgccttt ggaaaacgac acccgggtcg
     1201 actcctgcac ctcgggaaca tcgacccgcg cgtcggcaac cggacgcagg ctgctgcgga
     1261 acaggttgcc gatggcctgc ggcgactgcc gattggccag ggcgacgcgc gcgatcgtgt
     1321 ccatggtcaa ggcgcctcag ttagccgctt cgagcgcggc gatacgcgcc tcaagcggcg
     1381 gatgcgtggt gaacagcgca cgccacccgc cggcgcgatt gcccgagatg ccgaaggcgg
     1441 cgacgcggtc cggcagcgca ctcggctcgt ggctcgcctt caggcggcgc agcgcagcga
     1501 tcatccggct gcgaccggcg atgctggcgc caccggcatc ggcacggaat tcgcgctggc
     1561 gcgagaagtg catgactagc agcgaggcaa ggataccgag gccaatctcc atgatgatga
     1621 aggtggcgta gtaaccgaca cccaggcctc cttcattctt gagaaggacg cgatcgacaa
     1681 agtagccgac ggcacgggca atgacgatga cgaaggtgtt aaccacgccc tggatcaggg
     1741 cgagcgtcac catgtcgccg ttggcgatgt gcgagacctc gtgcgccagc acggcttcag
     1801 cctcgtcgcg cgtcatcttg cgcaacaacc cggtcgaaac ggcgaccagc gcgtgattgc
     1861 gcttcatgcc ggtggcgaag gcgttgacgt catcggcgtc gtagatcgcc acgtcgggca
     1921 tgccgatgcc cgccgcctgc gcctgccggc gaacggtggc gaccagccac aactcgaggt
     1981 cgttggccgg cgcctcgatg acgcgggcgc cgaccgactt catcgccacc catttcgaca
     2041 tcgccagcga gatgaaggcg ccaccgaagc caaacagtgc cgcaaagccg agcaaggcgc
     2101 cgaagtcaag gccggcggca gagacgaact ggttgaagcc gaacaggtgg gcgacgaccg
     2161 acagcgtcac cagcacggca aggttagtca tcagaaagag accgacacgt ttgaggttca
     2221 tggtcttact ccgtagaaaa cgtggccagc gttggcctgg gtttatcgga ctgggttcac
     2281 gagagcgatg cggttctcaa gcgatcatgt ccccctcctt cgccgatgcc gggtcggcgt
     2341 ggattgacgc agctcggcac gcagggcgcg gatttccgcg cgcagcgcgg ccacttgcga
     2401 atcgttaggc ggcggctcct cggccgcggc cgccgcctga ttctcgtgct ggatggcgtt
     2461 gacgatcacg gcgatgaaca ggttgagcaa cgtgaaggtc gaggcgagga tgaagaccag
     2521 gaagaaggcc caggcccacg ggtaaacctc
//
LOCUS       DATDAA010000336         2226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_269048,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000336 DATDAA010000000
VERSION     DATDAA010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269048"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VIS73_10200"
     CDS             <1..349
                     /locus_tag="VIS73_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="involved in the export of lipooligosaccharides
                     during nodulation; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nodulation factor ABC transporter ATP-binding
                     protein NodI"
                     /protein_id="HEY5763563.1"
                     /translation="FMDEAERLCDRLAVLDHGKVVVEGAPHELIASHIEPEVVEIFAV
                     ADEPGTIQRAVELCQALAARTEISGETAFLYCDDADPILARLHGDRSLRVMRRAANLE
                     DVFLKLTGRDLRD"
     gene            342..1166
                     /locus_tag="VIS73_10205"
     CDS             342..1166
                     /locus_tag="VIS73_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5763564.1"
                     /translation="MTETTADKAITAIRRRRADWLPELSWRWITVWRRNALVWRKMWA
                     ASVLGNLADPLIYMVGLGYGLGALVPKIDGAPYVVFLAAGTVCYSTMNAATFETLYSA
                     FSRMHVQKTWEALLNAPLSLDDVLLGELIWAATKSLLSGLAILLVIWILGLSSGGLSL
                     WVLPVIFVVGLSFSALGLIMNALAPGYDFFTYYFTLFITPMSLISGVFFPLDQLPGVI
                     RGIAEALPLSHGVALVRPLLKGEVPEAIGWHLLMLVATTAIAFLIARRLTRRRLLG"
     gene            1376..1891
                     /locus_tag="VIS73_10210"
     CDS             1376..1891
                     /locus_tag="VIS73_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5763565.1"
                     /translation="MAPVSRILVVDDEPVFADNLRDYLSRSSPDVRVAHDAQTALMAA
                     TEFSPEIVLMDCRLGAADGIAVLDRIRAERPDCRALLMTAHPREDVIRAADALGVSTV
                     LSKPFSFAELRDAMLACQVPTGSLEGFQERRIGERRQCEEASDSPTGFLQGLSARLER
                     RVKGGRRNSDG"
     gene            complement(1942..>2226)
                     /locus_tag="VIS73_10215"
     CDS             complement(1942..>2226)
                     /locus_tag="VIS73_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5763566.1"
                     /translation="VLLAPSAEVTPKQLAICPGLAPETGCDRNFQDADSYCMSIPQKG
                     VKLSEVERDLVIKALERTDWNVTKSAKLLGLSRDMLRYRIEKLELQRPPN"
BASE COUNT          358 a          722 c          720 g          426 t
ORIGIN      
        1 tttcatggac gaggccgaac ggctgtgcga ccgcctcgcc gttcttgatc acggcaaagt
       61 cgtcgtcgaa ggcgctccgc acgagttgat cgccagtcac atcgaacccg aggtcgtcga
      121 gatcttcgcc gtcgccgacg aaccggggac gatccagcgg gccgtcgaac tctgtcaggc
      181 gctggcggca aggaccgaga tctccggcga gactgctttc ctctactgcg atgacgccga
      241 tccgattctt gcccggctgc acggcgaccg ctcgctgcgc gtcatgcgcc gcgccgccaa
      301 cctcgaggac gtattcctca aactgaccgg gcgagacctg cgtgactgag acgacggccg
      361 acaaggcgat caccgcaata cggcgtcggc gtgccgattg gctgcccgag ctgtcgtggc
      421 gctggatcac ggtctggcgt cgcaacgccc tcgtctggcg caagatgtgg gcagcctcgg
      481 tgctcggcaa cctggccgat cccctgatct acatggtcgg gctcggctac ggactcggcg
      541 cactggtgcc gaagatcgac ggcgccccct acgttgtctt tcttgccgcc ggcaccgtct
      601 gctacagcac gatgaacgcg gcgaccttcg agacgctgta ctcggcgttc tcccgcatgc
      661 acgtgcagaa gacctgggaa gcgttgctca atgcgccgct ctcgctcgac gatgtactgc
      721 tcggcgaact gatctgggcg gcgaccaaga gcctgctttc ggggctggcg atcctgctgg
      781 tgatctggat tctcggccta tcttccggcg gtctctcgct gtgggtgctg ccggtgatct
      841 ttgtcgtcgg cctgtcgttt tcggcgctcg gtctgatcat gaacgcgctg gccccaggct
      901 acgacttctt tacctattac ttcacgctgt tcatcactcc gatgtcgctg atttccggtg
      961 tcttcttccc gctcgaccag ctgcccggcg tgattcgggg tattgccgag gcgctgccac
     1021 tttcgcacgg cgtcgcgctg gtacgcccgc tgctgaaagg ggaagtcccc gaggcgatcg
     1081 gctggcatct gctgatgctg gtggcgacga cggcgatcgc cttcctgatt gcccgtcggc
     1141 tgacccggcg gcgcctgctc ggctaggatt gcaccttggc tggggcaaat ccctcaccaa
     1201 ctttgggggg tgcgcccgaa ttggggtaca gaatgctgcc cgataagggt ccgatcggcc
     1261 ccatggcacg tgggtcccgg aaacgcaccg acgaccgccg gctggcatga agcatgctgc
     1321 cccgtatcgt cgccggcagt actaacaaac aacaaagcgc acgagggggg gccagatggc
     1381 accggtaagc cggattctag tggttgatga cgagccggtc tttgctgaca acctgcgcga
     1441 ttacctgtcg cgcagcagcc cagacgtgcg cgtcgcccac gatgcgcaga cggcgctgat
     1501 ggccgccacc gagttctcgc cggaaatcgt gctgatggac tgccggctcg gcgccgccga
     1561 cggcatcgct gtccttgacc gaattcgcgc cgaacgcccg gactgccgag cgttgctgat
     1621 gacagcacac ccgcgtgaag acgtcatccg cgccgccgac gcgctcggcg tatcgacggt
     1681 actgtccaag cccttctcgt tcgccgagct tcgcgatgcg atgctcgcct gccaggtgcc
     1741 gacgggatcg ctggaaggct ttcaggaacg gcgcatcggc gagcggcgcc aatgcgagga
     1801 ggcgtccgat tcgccgacgg gattccttca gggcttgtct gcgcgcctcg agcggcgcgt
     1861 caaaggcggc cgtcgcaaca gcgacggctg aaccgaggcg gacgcccacc gcggcgtccg
     1921 ggtcagcgca tgttcaggtg ttcagttggg tgggcgctgc aattcgagct tctcgatccg
     1981 gtagcgcagc atgtctcgcg agaggccgag cagcttggcc gacttggtga cgttccagtc
     2041 ggtccgctcg agcgccttga tcaccaggtc gcgctcgacc tcggagagct tgacaccctt
     2101 ctgcggaatc gacatgcagt acgagtcggc atcctggaaa ttgcggtcac aaccggtttc
     2161 gggcgcaaga cccggacaga tcgccaactg cttgggcgtt acctcagcgg agggggcgag
     2221 caggac
//
LOCUS       DATDAA010000337         4460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_409800,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000337 DATDAA010000000
VERSION     DATDAA010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409800"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..277
                     /locus_tag="VIS73_10220"
     CDS             41..277
                     /locus_tag="VIS73_10220"
                     /inference="COORDINATES: protein motif:HMM:NF019028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763567.1"
                     /translation="MKKAAAKPAAKKPAAKKPAAKKPAAKKVAAKPSPAPAASAPAAV
                     SAPAAAPSTAAAPGMKSALNPSAAWPFPSGSRPK"
     gene            complement(347..1513)
                     /locus_tag="VIS73_10225"
     CDS             complement(347..1513)
                     /locus_tag="VIS73_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY5763568.1"
                     /translation="MSATVRTDAIEPFHVVELLTRAKQLEAGGHDVIHMEVGEPDFPT
                     PPGIVAAATDALQRKPLGYTPALGIPELREAIADFYWHQHRVRISPARIVVTAGASGA
                     LTLACACLTAPGSRWLLPDPGYPCNRSFVRAFDGQPVPLATSAASNFQPTAEQVANAW
                     NERTGGVILASPSNPTGTVIDPAELAEIAATVHGRGGALVVDEIYSGLVYDSPPTTAL
                     AHPGIADEQLWIIQSFSKYWQMTGWRLGWLVVPEAHLRAVEKLAQNLFIAPSTPAQYA
                     ALAAFSAETLMLVEARRRAFKQRRDTLVPLLAELGFGVPAIPGGAFYVYADASALTTD
                     SYAYSRRLLETAHVAITPGIDFGQTSPERFVRFAYTTAAERITEAAERIARLCK"
     gene            complement(1629..3470)
                     /locus_tag="VIS73_10230"
     CDS             complement(1629..3470)
                     /locus_tag="VIS73_10230"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEY5763569.1"
                     /translation="MTASAAPSYVKHEALKKWVAEIAALTQPDQVYWADGSQEEYDRL
                     CAEMVTAGTLIKLNAQKRPNSYLAFSDPSDVARVEDRTYICSKNKDDAGPTNNWEDPA
                     KMRATLNGLFKGCMKGRTMYVIPFSMGPLGSPIAHIGVEISDSPYVVVNMRTMTRMGK
                     AVFDVLGTDGEFVPCVHTVGAPLEAGQQDAKWPCNPKTKYIVHYPETREIWSYGSGYG
                     GNALLGKKCFALRIASSMARDEGWLAEHMLILGVESPQGEKTYVAAAFPSACGKTNFA
                     MLIPPKSFNGWKIYTVGDDIAWIKPRKDKDGVTRFYAINPEAGFFGVAPGTSEKTNSN
                     ALATLKENVIFTNVALTDDGDVWWEGMTKEAPAHLIDWQGKDWTPEDGKAGRKAAHAN
                     SRFTAPASQCPSVDPNWEDPAGVPISAFIFGGRRATTVPLVYEAFNWNFGVYMAATLG
                     SETTAAAFGKQGVVRRDPFAMLPFCGYHMGDYFNHWLRMGHAVDESPRIFCVNWFRMN
                     EKGEFMWPGFGENMRVLKWIVDRVRGRVGAKETGLGWMPKFADIDWTGLDIDAKAFES
                     LTQVDANAWQQEIASHKDWFDKLGEKLPKELTLKRELFALGLWKDAA"
     gene            3722..>4460
                     /locus_tag="VIS73_10235"
     CDS             3722..>4460
                     /locus_tag="VIS73_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEY5763570.1"
                     /translation="MDQKQLREAALAYHRLPRPGKIAVTPTKQLTNQYDLSMAYSPGV
                     AAACEEIVADPSQASTLTSRANLVGVITNGTAVLGLGNIGALAAKPVMEGKGVLFKKF
                     AGIDVFDIELHQPDPDKLIEAIASMEPTFGGINLEDIKAPECFVIEQRLRELMDIPVF
                     HDDQHGTAIIATAGLINALLLTGRELKDCRMVVNGAGAASIACVELLKAMGLPHDHAI
                     LCDSKGVVYQGREEGMNQWKSAHAANTK"
BASE COUNT          807 a         1389 c         1436 g          828 t
ORIGIN      
        1 gaaaccagcc gcgaataagc cggcagcgaa gaagcctgcg gtgaagaagg cagcggcgaa
       61 acccgccgcg aaaaagccgg cagccaagaa gccggcagcc aagaagcctg cggcgaagaa
      121 ggtcgccgcc aagccttccc ctgcgccggc agcatcggca cctgcagcgg tttctgctcc
      181 ggctgcggcg ccctcaacgg cggctgcgcc gggcatgaag tcggcgctca atccgtcggc
      241 ggcctggccc ttcccatcgg gatcgcggcc gaagtaagcg gcgatcggat cgcggccatt
      301 gaggtcgcga tccaccgctc tccaagcgcg cctctggcgc gctttttcat ttgcagagcc
      361 tggcgatgcg ttcggcagcc tcggtgatcc gctctgccgc cgtcgtgtag gcgaagcgaa
      421 caaagcgttc gggcgatgtc tggccgaaat cgatgccggg tgtaatggct acgtgagccg
      481 tttcgagcag gcgccgcgaa tacgcgtagg agtcggtcgt cagcgccgat gcgtcggcgt
      541 agacgtagaa cgcgccgccg gggatggccg gaacgccaaa accgagttcg gcaagtagcg
      601 gcaccagtgt gtcgcgacgt tgcttgaagg ccctccgccg ggcttcgacg agcatcagcg
      661 tctccgcgct gaatgcggcg agcgcagcgt attgcgcggg cgtggacggg gcgatgaaca
      721 ggttttgtgc gagtttctcg accgcgcgca gatgtgcctc cggaaccact agccagccga
      781 ggcgccagcc ggtcatctgc cagtacttgg aaaagctctg gatgatccac agctgctcgt
      841 cggcaatgcc cggatgcgcc agcgccgtcg tcggcggcga atcgtagacc agccccgaat
      901 agatctcatc gaccacgagc gcgccgccgc ggccgtgcac ggtagcggcg atttcggcaa
      961 gctccgccgg gtcgatgacg gtcccggtgg gattcgacgg gcttgcaagg atgacgccgc
     1021 cggttcgttc gttccaggcg ttggcgacct gttcggccgt tggctggaaa ttgctcgcgg
     1081 cgctggttgc cagcggcacc ggttggccat cgaaagcacg aacgaaactg cggttgcatg
     1141 ggtagcccgg gtccggcagc agccagcgcg agcctggtgc ggtcaggcag gcgcaggcta
     1201 gggtcagggc gccggaagca ccggccgtga cgacgatccg cgccggcgaa atccgtaccc
     1261 ggtgctgatg ccagtagaaa tcggcgatcg cttcgcgcag ttccgggatg ccgagagccg
     1321 gcgtgtagcc cagcggtttt cgctgtagcg cgtcggtggc cgcagcgacg attcccggcg
     1381 gcgtcgggaa gtcgggctcg ccgacctcca tatggatgac gtcgtgaccg ccggcttcca
     1441 gttgcttggc acgggtcagc agttcgacga cgtggaaggg ctcgatggcg tcggtgcgga
     1501 cggtagcgct gatcacgata gcggagctcc gcggtttggc cggcaattgt tgctgcgccg
     1561 gaatggaaaa agccgcaacc cggattccgg atcgcggcct ctgccctggg gtttctggcg
     1621 cccgccgttc aggcggcgtc tttccacaag ccgagcgcaa acagctcgcg ctttagcgtc
     1681 agttcctttg gcagtttttc gccgagcttg tcgaaccagt ccttatggct ggcgatttcc
     1741 tgctgccagg cattggcgtc gacctgagtg aggctttcga atgccttggc atcaatgtcg
     1801 agtccagtcc agtcgatgtc ggcgaacttg ggcatccagc cgagcccggt ttccttcgcg
     1861 ccgacacgac cacgaacacg gtcgacgatc cacttgagca cgcgcatgtt ctcgccaaag
     1921 ccgggccaca tgaactcgcc cttctcgttc atgcggaacc agttaacgca gaagatccgt
     1981 ggcgattcgt cgaccgcgtg ccccatgcgc agccagtggt tgaagtaatc gcccatgtgg
     2041 tagccacaga acggcagcat cgcgaagggg tcgcggcgga ccacaccttg cttgccaaac
     2101 gcggccgccg tggtttcgga accaagcgtg gcagccatgt agacgccaaa attccagttg
     2161 aacgcttcgt agaccagcgg taccgtcgtc gcccggcgac cgccgaagat gaatgccgaa
     2221 atcggtacgc ccgccggatc ctcccagttc gggtcaaccg acgggcactg cgaggccggc
     2281 gccgtgaagc gcgagttggc gtgggcggcc tttcgaccag ccttgccatc ttcgggcgtc
     2341 cagtccttgc cctgccagtc gatcaggtgc gccggtgctt ccttggtcat gccctcccac
     2401 caaacatctc cgtcatcggt cagggcgacg ttggtgaaga tgacgttttc cttgagagtc
     2461 gccagcgcgt tggagttggt cttttccgat gtcccggggg ccacgccgaa gaagccggct
     2521 tccgggttga ttgcgtagaa acgcgtgacc ccatccttgt ccttgcgcgg cttgatccat
     2581 gcaatgtcat cgccaaccgt atagatcttc cagccgttaa acgacttcgg tggaatcagc
     2641 atggcgaagt tggtcttgcc gcaggccgac gggaaggctg ctgcgacgta tgtcttctca
     2701 ccctgtggcg actcgacgcc gaggatcagc atgtgctcgg cgagccaacc ttcgtcgcgc
     2761 gccatgctcg aggcaatgcg caacgcaaag cattttttgc cgagcaacgc gttgccgccg
     2821 tagcccgagc cgtaggacca gatttcgcgg gtttcgggat agtggacgat gtacttggtc
     2881 ttggggttgc agggccattt ggcatcctgc tggcctgctt cgagcggcgc gccaaccgtg
     2941 tgcacgcagg gaacgaattc accgtcggtg ccgagaacgt cgaacaccgc cttgcccatc
     3001 cgggtcatcg tccgcatgtt tacgacgacg tagggcgagt cggagatctc cacgccgata
     3061 tgagcgatcg ggctgccgag cggacccatc gaaaacggaa tcacgtacat cgtccgcccc
     3121 ttcatgcagc ccttgaacag gccgttgagg gtggcacgca tcttcgccgg gtcttcccag
     3181 ttgttggtcg ggccggcgtc gtccttgttc ttcgagcaga tgtaggtccg gtcctcgacg
     3241 cgggcaacgt ccgaggggtc ggagaacgcc agataggaat tcggccgctt ctgcgcgttg
     3301 agcttgatca gcgtgccggc cgtaaccatt tctgcgcaca ggcggtcgta ctcttcctgc
     3361 gatccgtcgg cccaatacac ttggtcgggt tgggtcagcg cggcgatttc agctacccat
     3421 ttcttgagcg cttcgtgttt cacatagctc ggcgcggcgg atgcggtcat ggaaggtctc
     3481 cgtggaaaat gaagaattcg gctgtccatc cgtgaaaacg gatgcgtcgg gcgatcggcg
     3541 actgggcatc gttgcgcagt cggcgccggt cggcgacagg gggttggtta cgggaaaacc
     3601 gtaattatgg cataaggctt ctggtcttag aatccttagc caaagccccg gagcgtccgg
     3661 cttcttgccg gccgatcgag caacgcgccg tccggcccat cacctcattc aggaaaacgc
     3721 aatggaccaa aaacaactcc gtgaggccgc tttggcctat caccgtcttc cccgtcccgg
     3781 caagattgcc gttacgccga ccaagcaact aaccaaccag tacgacctgt cgatggcgta
     3841 ctcaccgggc gtagccgccg cctgcgagga gatcgtcgcc gatccgtcac aggccagcac
     3901 gctgacctcg cgcgccaacc tggttggtgt catcaccaac ggcacggcgg tcctcggcct
     3961 tggcaacatc ggcgcgctcg ccgccaagcc ggtcatggaa ggaaagggcg tgctgttcaa
     4021 gaagttcgcc ggtatcgatg tcttcgacat cgaactgcac caacccgacc cggacaaact
     4081 gattgaggcg attgcctcga tggagccaac cttcggcggg atcaacctgg aggacatcaa
     4141 ggcgcccgag tgcttcgtca tcgagcagcg gctgcgtgaa ctgatggata ttccggtctt
     4201 ccacgatgac cagcacggca ccgcgatcat cgcgaccgcg ggcctgatca acgcgctgct
     4261 gctgaccggc cgcgagctca aggactgccg catggtggtc aacggcgccg gcgccgcctc
     4321 gatcgcctgc gtcgagctgc tcaaggccat ggggctgccg catgaccatg ccatcctgtg
     4381 cgattccaag ggcgtcgtct accagggccg cgaggagggc atgaaccagt ggaaatcggc
     4441 gcatgcggcc aacaccaagg
//
LOCUS       DATDAA010000338         2897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_130036,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000338 DATDAA010000000
VERSION     DATDAA010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130036"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(425..2212)
                     /gene="msbA"
                     /locus_tag="VIS73_10240"
     CDS             complement(425..2212)
                     /gene="msbA"
                     /locus_tag="VIS73_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEY5763571.1"
                     /translation="MSARPGSSAQQPDTPSGVLYRRLLQWVKPYWKQFALAVLCMVAT
                     AATEPMFPALMKYLLDNGFKAGSKAELWAIPVAMVAIFAVRGVAVYATTYGMAWISGR
                     VVMNLRAAMFDRLMTLPTRFYDDQSSGTLISKVAYDVINVSQAATNVITVIVRDTLTV
                     IGLLGFLLYLDWKLTMIALTVGPVIFGVIKIFSKRLRAASRNSYAAMGLITHILEEGV
                     GAHKVVKIFGGRDYESGRFHGAINRFRTAAIRETAAAASTVPLTQLAASFSVAIITWV
                     ALVQASSETGSAGSFIAFITALLMLLAPVKRLTDVNAPLQRGLAAAESIFELLDAEPE
                     DDSGKTVLPRSRGEIRFDGVHFSYLGANRAALSGIDLAVEPGRTVALVGPSGSGKTTL
                     AALIPRFYHASAGRVLIDGHNLEDVTLASLRASIALVSQDVVLFNDTVGANIAYGMAA
                     GASREAIIAAAEAANAWQFIREMPEGLDTIVGENGVKLSGGQRQRLAIARAFLKDAPI
                     LILDEATSALDSESERLIQGALESLMRHRTTIVIAHRLSTIENADLIVVLDGGRIVER
                     GRHDELLATKGVYARLWQLQHEVGGEIIP"
     gene            complement(2241..2816)
                     /gene="cobC"
                     /locus_tag="VIS73_10245"
     CDS             complement(2241..2816)
                     /gene="cobC"
                     /locus_tag="VIS73_10245"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase family protein"
                     /protein_id="HEY5763572.1"
                     /translation="MQLFLIRHPAPRIAPGTCYGRSDLELRESPAALAAEVATLLPAA
                     TCLFSSPLQRCRELALAISAEAVIDERLVEMDFGEWELRAWNEINRDGIDRWAAAPFD
                     FAPPGGESAMAMATRVIAFAREQLAPRGRSIAVVSHHGPLRVLAAIMLGQPRESWLSL
                     SFEQGTVSLVEVGDHGAAFRYRNHRPIAQHR"
     gene            complement(2801..>2897)
                     /locus_tag="VIS73_10250"
     CDS             complement(2801..>2897)
                     /locus_tag="VIS73_10250"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEY5763573.1"
                     /translation="RRLGGYTGDCLGATQQASELACYLGLACSFS"
BASE COUNT          491 a          900 c         1000 g          506 t
ORIGIN      
        1 ggtcaagccc aacggcaagc ccccctgaac ggccgcaatc gggcgataca cccatagacc
       61 gcttctggcc gcactcggtc ctccaagtac tggcggcgac tatatgacaa acgacacgcg
      121 caacaacgac tctcgcttcg cggacattgc tgttttccgc ccctgacggg ccgccagact
      181 ttctcttgtc ttgccgagag aaagtcggca aagagaaggc gcacccagtg tggcgccgca
      241 ctgcgcgagg tttcctgcgc tactcagccc cggcgtgggt tgcggaactc gccgctgtct
      301 cttcgcgagt caatagaaag cgtgcccgcg cgtcaggtgc cgaaaataac ggttacgaaa
      361 tccatagtcg accggtcggt tgcattgact ctatcggccg ggcgctcagg gtcaaactgc
      421 gcggtcaggg aatgatctcg ccgccgactt cgtgctgcag ctgccagagc cgcgcataga
      481 cgcccttggt cgcgagcagt tcgtcgtggc ggccgcgttc gacgatgcgt ccgccgtcga
      541 ggacgacgat caggtcggcg ttctcgatcg tcgataggcg gtgggcgatg acgatggtcg
      601 tgcggtggcg catcagcgat tcgagcgcgc cctggatcag gcgttcggat tccgagtcga
      661 gcgccgaagt cgcttcgtcg aggatcagga tcggcgcgtc cttgaggaag gcgcgggcaa
      721 tcgccagtcg ctggcgctga ccgcccgaca gcttgacgcc gttttcgccg acgatggtgt
      781 cgagcccttc gggcatctcg cggatgaact gccaggcgtt ggcagcttcg gcggcggcga
      841 tgatcgcctc gcggctggcg ccggcggcca tgccgtaggc gatgttggcg ccgaccgtgt
      901 cgttgaacag tacgacgtcc tgactgacca gcgcgatgct ggcgcgcagg ctggcgagcg
      961 tgacgtcttc gaggttgtgg ccgtcgatca gcacgcgccc ggcgctggcg tgatagaagc
     1021 gtgggatcag cgccgccagc gtggtcttgc cgctgcccga agggccgacc agcgcgacgg
     1081 tcctgcccgg ttcgactgcg aggtcaattc ccgagagcgc ggcgcggttg gcgccgaggt
     1141 agctgaagtg cacgccgtca aagcggatct cgccgcgtga acgcggcagt acggtcttgc
     1201 ccgagtcgtc ctcgggttcc gcgtcgagca gctcgaagat tgattcggca gcagcgaggc
     1261 cgcgttgcag cggcgcgttg acatcggtga ggcgcttcac cggcgcgagc agcatcagca
     1321 gggcggtgat gaaggcgatg aagctgcctg cgctgccggt ctcgctgctg gcctggacca
     1381 acgcgaccca ggtgatgatc gccaccgaga acgacgcggc tagctgggtc agcggcaccg
     1441 tcgacgcggc agcggcggtc tcgcggatcg ccgccgtgcg gaagcggttg atggcgccgt
     1501 ggaagcgacc tgattcgtag tcgcgaccgc cgaagatctt gaccaccttg tgcgcgccga
     1561 caccctcttc gaggatgtgc gtaatcaggc ccatcgccgc gtagctgttg cggctggcgg
     1621 cacgcaggcg cttgctgaag atcttgatga cgccgaagat taccggtccg acggtgaggg
     1681 cgatcatcgt cagcttccag tcgaggtaga gcaggaagcc gagcagcccg atcacggtca
     1741 gcgtgtcgcg gacgataacg gtaatgacgt tggtcgccgc ctggctgacg ttgataacgt
     1801 cgtaggcgac tttcgagatc agcgtgcctg acgactggtc gtcgtagaag cgtgtcggca
     1861 gggtcatcag gcggtcgaac atcgccgcgc gcaggttcat caccacccgg cccgagatcc
     1921 acgccatgcc gtaggtggtg gcgtaaacgg cgacaccgcg aaccgcgaaa atggcgacca
     1981 tcgccaccgg gatcgcccac agttcagcct tgctgcctgc cttgaagccg ttgtcgagca
     2041 gatacttcat cagcgccggg aacatcggct cggtcgctgc cgtcgcgacc atgcacagca
     2101 ccgccagcgc aaactgcttc cagtagggct ttacccattg cagcagccgg cgatagagca
     2161 cgccgctggg cgtgtcgggt tgctgggcgc tgctgccggg tcttgcggac atggtttctc
     2221 gattcaagga gctacgcggg tcagcggtgt tgggctattg gtcggtgatt ccggtaacga
     2281 aaagcggctc catggtcacc cacctcgacc aggctgaccg taccctgttc gaacgacagg
     2341 ctgagccagc tttcgcgggg ctggccgagc atgattgccg ccagtacgcg caagggaccg
     2401 tggtgggaaa ccacggcgat gctgcggccc cggggggcca gctgttcgcg cgcgaaggct
     2461 atcacacggg tcgccatggc cattgccgat tcgcctcccg ggggtgcgaa gtcgaaaggg
     2521 gcggcagccc agcggtcgat gccgtcgcga tttatctcgt tccatgcgcg caattcccac
     2581 tcgccgaaat ccatctcgac gaggcgctcg tcgatcacgg cttcggcgct gatggccaat
     2641 gccagttcgc ggcagcgttg cagggggctg ctgaacaggc acgtcgcggc tggcagcaac
     2701 gtggccacct cggcggcgag tgctgcaggc gattcgcgca gctcgagatc gctgcgcccg
     2761 tagcaggtgc ccggcgcgat ccggggcgcc gggtggcgga tcaggaaaag ctgcacgcga
     2821 ggccgaggta gcaggcgagt tcgctggcct gctgcgtcgc gcctaggcag tcgccggtgt
     2881 aaccgccgag gcggcgg
//
LOCUS       DATDAA010000339         2384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_24688,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000339 DATDAA010000000
VERSION     DATDAA010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24688"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VIS73_10255"
     CDS             complement(<1..491)
                     /locus_tag="VIS73_10255"
                     /inference="COORDINATES: protein motif:HMM:NF020134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763574.1"
                     /translation="MTTMGIVATGTWFPDTCVTAAEIAQRSGIPEPVVREKLGIERKF
                     VADPRVHPNQMAVNAARKAIDAAGIDPKDIDVVLCTTEEWREYLLWTTAIDLAWEIGA
                     RKAWGVDVHTRCATTIAALKMARGLLASDPSIDTILVGGGYAVSHFIDLGDVDTSFLF
                     NIGA"
     gene            complement(496..1098)
                     /locus_tag="VIS73_10260"
     CDS             complement(496..1098)
                     /locus_tag="VIS73_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5763575.1"
                     /translation="MSSYGRRPKTDRGRKTRNRLLEAAEIEFGEKGFHEAAISGITQR
                     AGVALGTFYTYFESKQEIFTALVSFMSERTRQWVAERVAGATDRLAAERLGLAAYIEF
                     VRQHKGIYRIMSEAEFIANDAFRAHYEGFARSYCANLDKAGERGEIRRGDYEVWSWAI
                     IGINVFLGMRFAEWDEGEAPLRIAGSVLDLIGNGMSRDEA"
     gene            1233..>2384
                     /locus_tag="VIS73_10265"
     CDS             1233..>2384
                     /locus_tag="VIS73_10265"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY5763576.1"
                     /translation="MKEKRGSRNLLIAVAVAAALPAPVLAQQPEVAALEGIVVTSRRM
                     EESLQDVPGAVSAFSEQTIERIGAADSTGLQGLVPNLNIVQGRGSSNATNIYIRGVGQ
                     PDALQTFDPAVGFYVDDVYYSRIRGTQLDLFDIERIEVLRGPQGTLYGKNTIGGALKV
                     ITRRPSQERQTMLQATAGNYGQLEGRISVSGPLGESLAGGVSLFSAQRNGYVTNPTTG
                     AKYNDRDVQAGRVALSWKPSENVAVDWSADYADEDNALVLGQPINTLTTLFGVPILVL
                     PANVPEFRFTATPTPGLPNSTKLTHQGTALNVAWEVSEALTLRSITAYRELESTDYID
                     IDATVIETGDVLVDVDQDQISQEFQANYVWERLSFVGGLYYLRENVESHQ"
BASE COUNT          417 a          796 c          764 g          407 t
ORIGIN      
        1 gcgccgatgt tgaacaggaa ggaggtgtcc acgtcgccca ggtcgatgaa gtgggagacg
       61 gcatagccgc cgcccaccag gatggtgtcg atggaagggt cgctggccag caggccccgg
      121 gccatcttga gcgcggcgat cgtggtcgca cagcgggtat gcacgtcgac gccccaggcc
      181 ttgcgtgcgc cgatttccca ggcgaggtcg atggccgtgg tccacagcag gtactcgcgc
      241 cactcctccg tggtgcacag gaccacgtcg atgtccttgg ggtcgatgcc ggccgcatcg
      301 atggccttgc gggccgcatt gacggccatc tggttcgggt ggacccgcgg atccgccacg
      361 aacttgcgct cgatgccgag cttctcgcgg accaccggct cggggatgcc gctgcgctgc
      421 gcgatttccg ccgcggtgac gcaggtgtcc gggaaccagg tgccggtcgc gacgatgccc
      481 atggtggtca ttgcgtcatg cctcgtcccg cgacatcccg ttgccgatca ggtccagcac
      541 cgacccggcg atccgcaagg gcgcttcgcc ctcatcccac tccgcaaatc gcatgcccag
      601 gaagacgttg atgccgatga tcgcccagga ccagacctcg taatcgcccc ggcggatctc
      661 gccgcgctcg cccgccttgt cgaggttcgc gcagtaggag cgggcgaacc cttcgtagtg
      721 cgccctgaag gcgtcgttgg cgataaactc ggcctccgac atgatccggt agatgccctt
      781 gtgctggcgg acgaactcga tgtaggcggc caggccgagc cgctccgctg ccagccggtc
      841 cgttgcgccg gcgacccgct cggcgaccca ttgccgcgtt cgctcgctca tgaaggaaac
      901 gagggctgtg aagatctcct gcttgctttc gaagtaggta tagaaggttc cgagcgccac
      961 gcccgcccgt tgcgtgatgc cgctgatagc ggcctcgtgg aagccctttt cgccgaactc
     1021 gatctcggcc gcctcgagca ggcgattgcg cgtctttcta ccccgatcgg ttttcggacg
     1081 gcggccataa cttgacactt gattcacgtt tcataatctt gcattctttc gccaggatgg
     1141 caaggcgtca ttgctcattg ggcgatcctg gatcgagagc cgcggctgcg gcgccagcat
     1201 cgcgctaaca caacactgtc aaggggaatt gcatgaaaga gaagcgcggt tccaggaacc
     1261 tgctgatcgc ggtcgccgta gccgctgcgc tgccggcacc cgtgctcgcc cagcagccgg
     1321 aagtggcggc cctggagggg atcgtggtca cgtcgcggcg catggaagag tcgctgcagg
     1381 acgtgcccgg cgcggtgtcg gcgttctccg agcagaccat cgagcgcatc ggcgcggccg
     1441 acagcaccgg gctacagggc ctagtgccca acctcaacat cgtgcagggc cgcggctcat
     1501 ccaatgcgac caacatctac atccgcggcg tgggccagcc cgatgcgctg cagacgttcg
     1561 atccggcggt cggtttctac gtcgatgacg tctattactc gcgcatccgg ggtacgcagc
     1621 tggacctgtt cgacatcgag cggatcgagg ttctgcgcgg cccgcagggc accctgtacg
     1681 gcaagaacac gatcggcggc gcgctcaagg tcatcacgcg ccggcccagc caggaacgtc
     1741 agaccatgct gcaggcgacg gccggcaact acggccagct ggaaggccgc atctcggtct
     1801 ccggccctct cggcgagtcg ctggccggag gcgtgtcgct gttcagtgcg cagcgcaacg
     1861 gctatgtcac caacccgacg acgggtgcga aatacaacga tcgcgacgtc caggccggcc
     1921 gcgtggccct gtcctggaag ccgtcggaaa acgtggcggt cgactggtcc gccgactacg
     1981 ccgacgaaga caacgcgctc gtgctgggac agccgatcaa caccctgacg accctgttcg
     2041 gcgtgccgat cctcgtgctg cccgccaacg tcccggaatt caggttcacc gccacgccga
     2101 cgcccggcct gcccaactcg acgaaactca cccaccaggg tacggcgctc aacgtggcat
     2161 gggaggtcag cgaggccctc accctgcgtt cgatcaccgc gtatcgcgaa ctggaaagca
     2221 ccgactacat cgacatcgac gcgacggtca tcgaaaccgg cgacgtcctc gtcgacgtcg
     2281 atcaggacca gatcagccag gaattccagg ccaactatgt ctgggagagg ctgagcttcg
     2341 tgggcggact ctactatctg cgcgagaacg tcgagtcgca ccag
//
LOCUS       DATDAA010000340         2158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_24690,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000340 DATDAA010000000
VERSION     DATDAA010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24690"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(423..1226)
                     /locus_tag="VIS73_10270"
     CDS             complement(423..1226)
                     /locus_tag="VIS73_10270"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY5763577.1"
                     /translation="MNPPMLVNPLAERLKDGGLGLALMIRHARTVDIALAARTCGFDA
                     LYFDLQHTPTPEEVVAQISVAAIGAGVTPLVRVPDRDYAAALRMLDSGALGIVMPDVS
                     SAEDARHAVSHCKFAPLGNRGAYGSWPQLGYRALPALEARQALNDATLLIVMIENQAA
                     LENVEAITAVPGVDIVHVGSNDLATDLGVAGELTHPKVLAAIERVIAACRKDGKVPGV
                     GGLTGGDPRRIEQVVRLGARFLTAATEWNLMLSAGQERVKALRALEQKA"
     gene            complement(1386..>2158)
                     /locus_tag="VIS73_10275"
     CDS             complement(1386..>2158)
                     /locus_tag="VIS73_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5763578.1"
                     /translation="IHHPDVRRMLLSMKAQTEAMRALAYFAAALLDRSKHHPDAAQRR
                     RDQAMLDLLTPVVKGWCTEQAIEIASTGVQIHGGMGFIEEAGAAQYLRDARITTIYEG
                     TTGIQANDLVGRKVGAEAGATALALIKAMRSVASKLEAGPFADTRGSLKDAIDGLEQA
                     TRWIVDSFPRDAKAVAAVSVPYLKLFGTVSGGWMMARAALIASEQLKQPDADQGFLEA
                     KLATARFYAEHELPKAAALAREVIHGAASVLALDPAKF"
BASE COUNT          379 a          705 c          668 g          406 t
ORIGIN      
        1 ttacgtcgcc ggtgagcggt gacgggcaat gccggcgacg cgctacagct tggccgccaa
       61 gtccgctagg gtcgagaagg aagaaggaag ctttaccgca acggacgcca agaacgcgga
      121 gtaaaagaaa agctttgaca caaaggacac taaggttaca acggattaca agcgccaatt
      181 agaagacacg gcgagcgtcg tctccggagc accggtattc ccgtatgcca gcctgagcgc
      241 tgcctttgcg aacttggggt gcgtactggt cgctgttcct tggcgctctt ggcgcccttt
      301 gcggtgaggc cgttatcttc ctggcgccct ccgcggtaag gctttgcggt cctggccgga
      361 gtttggctca cgattcacga tttacagccg cttcagtcca cccgctacgc atcatccact
      421 attcacgctt tctgctcgag agcgcggagc gctttgacgc gctcctgccc ggcggacagc
      481 ataaggttcc attccgttgc ggccgtgagg aagcgggcgc ccagtctcac cacctgctcg
      541 atgcgcctgg gatcaccccc cgtcaaaccg ccgacacctg gaactttgcc gtccttgcgg
      601 cacgccgcaa tcacccgctc gattgcggcg agcaccttgg gatgagtgag ctcaccggcg
      661 acaccgagat cggtggcaag atcgttgctg cccacgtgca cgatgtcgac ccccggcacg
      721 gcagtgatcg cctccacgtt ctcgagcgcc gcctggtttt cgatcatgac gatgagcagc
      781 gtcgcgtcgt tcagtgcctg gcgcgcctcg agcgccggta gcgcccggta gcccagctgc
      841 ggccagctgc cgtaggcacc gcgattcccg agcggcgcga acttgcagtg actgacggca
      901 tggcgcgcgt cctcggcgga agatacgtcc ggcatgacaa tgccgagggc gccgctgtcg
      961 agcatccgca gcgctgcagc atagtcgcgg tcgggaacgc gcaccagcgg ggtgacaccg
     1021 gcaccgattg cggcgaccga gatttgggcg acgacttcct ctggcgtggg cgtatgctgc
     1081 aaatcgaagt agagcgcgtc gaagccgcac gttcgcgcag ccagcgcaat atccaccgtg
     1141 cgcgcatgcc ggatcatcag ggcgagcccc agcccgccgt ccttaagccg ctcggcgagc
     1201 gggttcacta acatgggggg attcaaagcc atctcctatc ctttgaaatg cattacgggc
     1261 gacgggtccg acacccgtga gtgttaaatg gtgaattttg attgttgaat ggagttttgc
     1321 tctgcttggc ggcttggcag ctcaatacca gttgttgctt ccctcggcgc cccggcggtt
     1381 cctcgctaaa actttgcggg atcaagagcc agcacgctcg ccgcgccgtg gatgacctca
     1441 cgcgcgagcg cagccgcttt cggcagctca tgctccgcgt agaacctggc ggttgcgagc
     1501 ttggcttcga gaaagccctg gtccgcgtcg ggttgcttga gctgctcgct ggcgatcagc
     1561 gcggcgcggg ccatcatcca cccccccgag acggtgccga aaagcttgag gtacggcacc
     1621 gaaacagcgg caaccgcctt tgcgtcccgc ggaaagctat ccacaatcca gcgcgtcgcc
     1681 tgctccagcc cgtcaatcgc gtccttcaga ctgccgcgcg tgtcagcgaa gggcccggcc
     1741 tccagctttg atgccacgct gcgcattgcc ttaatcagcg ccagcgccgt cgcgccggcc
     1801 tcggcgccga ccttgcgccc gacgagatcg ttcgcctgga tgccggtcgt gccttcatag
     1861 atggtcgtaa tgcgcgcatc ccgcaggtat tgcgcggcgc cagcctcctc gatgaaaccc
     1921 atgcccccgt gtatctggac gccggtcgag gcaatctcga tggcttgctc ggtgcaccag
     1981 cctttgacga ctggcgtgag cagatccagc atcgcctgat cacgccgcct ctgcgcggca
     2041 tcggggtggt gcttcgacct gtcgagcaac gcggcagcga aatacgccag ggcgcgcatc
     2101 gcctccgtct gggccttcat cgacagcagc atccggcgca cgtcgggatg atggatga
//
LOCUS       DATDAA010000341         2012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_59692,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000341 DATDAA010000000
VERSION     DATDAA010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59692"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /gene="dtd"
                     /locus_tag="VIS73_10280"
     CDS             <1..346
                     /gene="dtd"
                     /locus_tag="VIS73_10280"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY5763579.1"
                     /translation="DGESDLVWTAQKLVRLRVFADAQGVMNDSVVDVGGALLVVSQFT
                     LFAATRKGTRPSWGRAAAPELARPMFDRFVGLLEKELGRPVPTGVFGAEMAVDLCNDG
                     PVSLMLDSKLRE"
     gene            386..>2012
                     /locus_tag="VIS73_10285"
     CDS             386..>2012
                     /locus_tag="VIS73_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY5763580.1"
                     /translation="MEETLFGLMLVMTAAVLTVALARALGLPSLLAYLAVGVALGPHG
                     LRLVEESEQAAHLAEFGVVFLMFSIGLEFSMARLKSMRFLVFGMGGAQMGLTMLATIA
                     GTMFYFGQDWRVGLTIGAVCAMSSTAIVAKMLSERFELHTAAGRQTMGVLLFQDLAVV
                     PLLILIPALAAPAGDLAFTLGQALVIAIITLLLVGIIGPRVMRAWFDRVAKGRSDELF
                     MLSVLWMVLVLAGITAWAGLSMALGAFLAGVLISETRYRHQVEADVRPFRDVLLGVFF
                     LTIGMRLDLYYVITHLPQTALAVLLLVLAKGGIVFLISMAIRSPLHVALRTAAQLAQA
                     GEFGFVLIELGGQRGLIPEDVFQVTIAAMLLSMFLAPVLINRAGGVVNRLQRGAWAND
                     ARVVHAVAAHSMDLKDHVILCGYGRAGQNVARFLDSEQIPYLALDLDAERIRFAEGQQ
                     QQVVFGNAERAEVLTAAGLKRARAVIITYPDAASAELVVQRVRRQRKDIPVVVRATDD
                     SALDRLRNAGATEVVPEVLEGSLMLGVHALAELG"
BASE COUNT          284 a          623 c          690 g          415 t
ORIGIN      
        1 cgacggcgag tcggatctcg tctggaccgc acagaagctg gttcgccttc gcgtattcgc
       61 cgatgcgcag ggcgtgatga acgactcggt agtcgacgtc gggggagcac ttctggtggt
      121 cagccagttc acgctgttcg ctgccacgcg caagggaacg cggccatcgt gggggcgggc
      181 tgcagcgccc gaacttgcgc gacccatgtt cgaccgtttt gtcgggctgc tggaaaagga
      241 actcgggcgc ccggtgccaa ccggcgtatt cggcgccgaa atggcggtag atttgtgcaa
      301 cgacggcccg gtaagcttga tgctggattc gaagctgagg gaatagggcg gcggtaatcg
      361 cggctcgcgc ggagcgcttc aagtcatgga agaaacgctg ttcggactga tgctggtgat
      421 gaccgcggcc gtgctgaccg tggcgctggc gcgcgcactc ggtcttccct ctctgctagc
      481 ctatctcgcc gtcggcgttg ccctcgggcc gcatggtctg cgcctggtgg aagagagcga
      541 gcaggctgcg catctggccg aattcggggt tgtctttctg atgttctcga tcggcctcga
      601 attcagcatg gcgcgattga agtcgatgcg ttttctggtc ttcggcatgg gcggtgccca
      661 gatgggcctg accatgctgg cgacgattgc cggcacgatg ttctacttcg gtcaggactg
      721 gcgcgtcgga ctgacgatcg gcgcggtctg cgcgatgtcg tcgacggcga tcgtcgccaa
      781 gatgctctcc gagcgtttcg agctgcacac cgccgccggg cggcagacca tgggcgttct
      841 gctgtttcag gatctcgccg tcgtgccgct gctgatcctg attccggcgc tggcggcacc
      901 ggccggcgat ttggccttca ccctcggtca ggccttggtc attgcgatca ttacgctgct
      961 gctggtcggc atcattgggc cgcgcgtcat gcgcgcctgg ttcgaccgcg tcgccaaggg
     1021 gcggtccgat gagctgttca tgctcagcgt gctgtggatg gtgctggtgc tggccggaat
     1081 caccgcctgg gctgggctgt cgatggcact gggtgccttc ctcgccggcg ttctgatttc
     1141 ggagacgcgt taccggcacc aggtcgaggc cgacgtcagg ccgttccgcg atgtgctgtt
     1201 gggtgttttc ttcctcacga tcggcatgcg cctcgatctt tattacgtca ttacccacct
     1261 gccgcagacc gctctggccg tgttgctgct ggtgctggcc aaggggggca tcgtcttcct
     1321 gatctcgatg gcgatccgca gtccgctgca cgttgccctg cgcaccgcgg cccaactggc
     1381 gcaggccggt gagttcggtt tcgtgctgat cgaattgggc ggccagcgcg gcctgattcc
     1441 cgaggacgtg tttcaggtga cgatcgccgc gatgctgctg tcgatgtttc tcgcgccggt
     1501 gctcatcaat cgcgcgggcg gggtggtcaa ccgcctgcag cgcggcgcct gggccaacga
     1561 cgcgcgggtc gtccatgcgg ttgccgctca cagcatggac ctcaaggatc acgtcatcct
     1621 ctgcggttac gggcgggccg gtcagaacgt ggcgcgcttc ctcgactccg agcagatccc
     1681 ttacctcgcg ctcgacctcg acgccgagcg catccgcttt gccgaaggcc agcagcagca
     1741 ggtggtgttc ggcaatgccg aacgcgccga agtactgacg gccgccggtt tgaaacgcgc
     1801 ccgcgcggtc atcattacct acccggatgc ggcgtcggcc gagctcgtcg tccagcgagt
     1861 ccggcgacag cgcaaggaca tcccggtggt ggtccgggcg accgacgaca gcgcgctcga
     1921 ccggctgcgc aatgccggcg ctaccgaggt cgtccccgaa gtcctcgaag gcagcctgat
     1981 gctcggcgtg cacgcgctgg cagaattggg tg
//
LOCUS       DATDAA010000342         6032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_130428,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000342 DATDAA010000000
VERSION     DATDAA010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130428"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VIS73_10290"
     CDS             complement(<1..644)
                     /locus_tag="VIS73_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY5763581.1"
                     /translation="MLDLFGRHAAHEAKRQLAAQQSVNESLTAKLDAMKAEKDTLTRE
                     LDSLRKERQAFSALFENFRAYGDSLIETQQTLASLATRLREEKQETVAAATLSAESRD
                     AIQRIAKSLGRLASDSTQAVTQVASLHASAEKIGGIVNLIKEIADQTNLLALNAAIEA
                     ARAGEAGRGFAVVADEVRKLAERTTNATSEISGLVGEIQRETADAQASMGDLSA"
     gene            complement(746..1252)
                     /gene="folK"
                     /locus_tag="VIS73_10295"
     CDS             complement(746..1252)
                     /gene="folK"
                     /locus_tag="VIS73_10295"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY5763582.1"
                     /translation="MSAAPPVTAFIALGANLDDPQRQLKGAVARLAGLTDSRLIRHSS
                     LYRTAPVGVGPQPAFVNAVAEVETRLPADALLGELLEIEQQAGRVRLQQGAPRTLDLD
                     LLLYGGQCLRRPGLAVPHPRMHLRAFVLVPLLEIAPDAQIPGRGSAAAWYPVVYRQAI
                     ERLAESHH"
     gene            complement(1249..2616)
                     /gene="pcnB"
                     /locus_tag="VIS73_10300"
     CDS             complement(1249..2616)
                     /gene="pcnB"
                     /locus_tag="VIS73_10300"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEY5763583.1"
                     /translation="MIRKFISRVFSRRKPAERKGPAMVKASAHRLRPEQLSQGARRTI
                     EGLQAKGFEAYVVGGAVRDLLLGLVPKDFDIATDATPEEVRATFRRSRVIGRRFQIVH
                     VMMGAETLEVTTFRGPQDERHVQKDSHGRILRDNVFGGIEDDAARRDFTVNALYYDPK
                     RDLIVDYHHGVADLGARTLRMIGKPELRYREDPVRMLRAVRLAAKLGLQIDPQTRQPI
                     RVMADLLENVPPARLFDEMLKLLTSGHSVACIEQLRADGLHHGLLPLLDVILGQPLGE
                     RFVMLALAKTDARVRNEQSISPGFLFATLLWHEVMASWQKRQEAGEHTFPALFEAMDE
                     VLDQQSEKLAITKRIAGDIKEIWGLQARFDQRSGKRPHGLLAHPRFRAGYDFMLLRVE
                     AGEAEAELGDWWTDFLDAEGPAREAMLLPASGEKKKRRRRRRKPRAGGGSDDGAGKAE
                     PPDAE"
     gene            complement(2731..3414)
                     /locus_tag="VIS73_10305"
     CDS             complement(2731..3414)
                     /locus_tag="VIS73_10305"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5763584.1"
                     /translation="MTTPAAPLNLALFDLDNTLLDGDSDFEWAQFLIARGVLDREVHE
                     AKNLAFFEAYKAGTLDIHAFLDFQLAPLARHPRAQLDAWHREFMAERIAPIIGEKARH
                     LVDGHRSRGDLCALVTATNSFVTGPIARAFGIAHLIATIPAQVDGRFTGKPAGTPSFR
                     EGKVTRVDAWLEAHGLWWGSFAKTSFYSDSLNDLPLLARVSDPVAVDADPTLAAHASA
                     HGWRTMSLR"
     gene            complement(3411..4133)
                     /locus_tag="VIS73_10310"
     CDS             complement(3411..4133)
                     /locus_tag="VIS73_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEY5763585.1"
                     /translation="MSTAAGTTEAQPGRQLLLDLLPPPAPNFDNFIVGGNAEALAALR
                     EWLSLPGSPTCFLLWGEAHSGRRHLLAASGLPLVDAAATELDVAAALAAQTAPALAVA
                     NADRLDAAGQTALFVAFNHRRQNRGKLLLSSVQPPAALAIREDLRTRLGSGLVYRLNA
                     LSDAEKSAALLARAEALGLELPAGTLDFLFSRSARDLGTLTALIDALDRYTLEHRRPV
                     TLPLLRRLLESEPNPEPLTVNR"
     gene            complement(4144..5316)
                     /locus_tag="VIS73_10315"
     CDS             complement(4144..5316)
                     /locus_tag="VIS73_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY5763586.1"
                     /translation="MAHGPEQPAQASPDSGTARADATSSPAAAVQSAAGGAPDGTLLL
                     RQIIPVVVVAALLWLLVALAPVLEPFAAAAMLAYICNPLVERLEALGIGRAFGSVLTT
                     VLLLLAFLGFLLVVVPLFIEQSARLADRLPVLLRAFGERGLPWLNEKLGGAIPLDLAS
                     LADLARQHAESLRGAAAKAFVALSQRGAAVAGLLGLLLLVPVVTWYLLIDWPRITFAA
                     QSLAPKRWQARISEVTGEIDHVLGQFMRGQLSVMLALATFYSIALAVAGIEYALALGL
                     ITGLLCFIPYVGFGLGLILALLSAFLQNEGWQPFVWVGAIFGLGQLIESFFLTPYLVG
                     ERIGLHPVVVILALLVFGQLLGFFGVVIALPVSAVLLVALRHALAAYRASDFFSRQ"
     gene            5478..>6032
                     /gene="purM"
                     /locus_tag="VIS73_10320"
     CDS             5478..>6032
                     /gene="purM"
                     /locus_tag="VIS73_10320"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY5763587.1"
                     /translation="MSIPATPLSYRDAGVDIDAGDALVDRIKPLAKKTMRDGVLAGIG
                     GFGALFEVPKRYREPVLVSGTDGVGTKLKLAFQLKRHDTVGQDLVAMSVNDILVQGAE
                     SLFFLDYFACGKLDVDTAAAVVGGIARGCELAGCALIGGETAEMPSMYPDGEYDLAGF
                     AVGVVEKAKIIDGRTIVPGDVVLGL"
BASE COUNT          952 a         1908 c         2188 g          984 t
ORIGIN      
        1 tgcgccgaga ggtcgcccat gctagcctgc gcgtcggccg tctcgcgctg gatctcgccg
       61 accagtccgg agatctcgct ggttgcgttg gtggtccgct cggcgagctt gcgcacctcg
      121 tcggcgacca ccgcaaagcc gcggcctgcc tcgccggcgc gggcggcttc gatggccgca
      181 ttgagcgcca gcaaattggt ctggtcggcg atctccttga tcaggttgac gatgccgccg
      241 atcttttccg cgctggcgtg caggctggcg acctgcgtca cggcctgcgt ggagtcgctc
      301 gccagacggc cgaggctctt ggcgatgcgc tggattgcgt cgcggctctc ggccgacagc
      361 gtggccgcgg cgacggtctc ctgcttctct tcgcgcaggc gcgtggcgag gctggcgagc
      421 gtttgctgcg tctcgatcag cgagtcgccg taggcgcgga aattctcaaa cagcgccgaa
      481 aacgcctgcc gctccttgcg gagcgaatcc agttcgcggg tcagcgtgtc cttttctgcc
      541 ttcatggcgt cgagcttggc ggtcagcgat tcgttgacgg attgctgggc agcgagctgg
      601 cgcttcgcct cgtgcgctgc atggcggccg aatagatcaa gcatggcggt tcctcgatgg
      661 ggcgttttgg gctggcgcgg ccggtttcgg ttgctggcct gcgggcgctc aagggcgcgt
      721 agtttacccg atgcctgctc tcactttagt ggtggctttc cgcgagccgc tcgattgcct
      781 gccgatacac caccggatac caggccgctg cgctgccccg gccaggaatc tgggcgtcgg
      841 gggcgatttc gagcagcggc acgaggacga aggcgcgcag gtgcatgcgc ggatgcggta
      901 cggccagtcc gggccggcgc aggcattggc cgccgtagag cagcaggtca aggtcgagcg
      961 tgcgtggcgc cccctgttgg agccgtacgc ggccggcctg ttgttcgatt tcgagcagct
     1021 ccccgagcag ggcgtcggcg ggcaggcggg tttcgacttc ggcaacggca ttgacgaagg
     1081 ccggctgtgg cccgaccccg accggcgccg tgcggtagag agaagaatgg cggatcaggc
     1141 gcgagtcggt gagacccgcg aggcgggcaa ccgcgccctt gagctgccgt tgcggatcgt
     1201 cgaggttggc gccgagtgcg atgaaggcag tgaccggcgg cgccgcgctc attccgcgtc
     1261 gggcggctcg gccttgcccg cgccgtcgtc ggaaccgccg cctgcacgcg gcttgcggcg
     1321 acgacgacga cgtttcttct tctcgccgct ggccggcagc agcatcgcct ctcgtgccgg
     1381 gccttcggcg tcgaggaagt ccgtccacca gtcgccgagt tcggcttcgg cctcgccggc
     1441 ctcgacccgc aacaacatga agtcgtaacc ggcgcgaaag cgcgggtggg caagcaggcc
     1501 gtgggggcgc ttgcccgaac gttggtcgaa gcgcgcctgc agtccccaga tctccttgat
     1561 gtcgccggca atccgcttgg tgatggcgag tttttccgat tgctggtcga gcacctcgtc
     1621 catcgcctcg aacagtgccg gaaaggtgtg ttcgcccgct tcctgccgct tctgccagct
     1681 ggccatgact tcgtgccaga gcagcgtggc gaagaggaat cccggcgaga tcgactgctc
     1741 gttgcgcacg cgcgcatcgg tcttggccag cgcgagcatg acgaagcgct cgccgagcgg
     1801 ttgcccgaga atcacgtcga gcagcggcag caatccgtgg tgcaggccgt cagcgcgcag
     1861 ctgctcgatg caggcgaccg agtggcccga ggtcaggagc ttgagcatct cgtcgaacag
     1921 ccgtgccggt ggcacgtttt cgagcagatc ggccatgacc cggatcggct gccgcgtctg
     1981 cgggtcgatc tgcaggccga gcttggctgc caggcgaacc gcgcgcagca tgcgtaccgg
     2041 gtcttcgcgg tagcgcagtt cgggcttgcc gatcatgcgc agggtgcggg cgccgaggtc
     2101 ggcgacgccg tggtggtagt cgacgatcag gtcacgcttc ggatcgtagt agagcgcgtt
     2161 gacggtgaag tcgcggcgcg ccgcgtcgtc ctcgatgcca ccgaagacgt tgtcgcgcag
     2221 gatgcggccg tgcgagtcct tctgcacgtg gcgctcgtcc tgcggcccac ggaaggtggt
     2281 tacctccagg gtttccgcac ccatcatcac gtggacgatt tggaagcggc ggccgatcac
     2341 gcgcgagcgg cggaaggtgg cgcgcacctc ttcgggtgtc gcgtcggtgg cgatgtcgaa
     2401 atccttgggg acgagaccga gcagcaggtc gcgtaccgca ccgccgacca cgtaggcctc
     2461 gaaacccttg gcctgcaggc cttcgatcgt gcgtcgcgcg ccctgcgaca gctgctcggg
     2521 gcgaaggcgg tgcgccgagg ccttgaccat cgccgggcct ttgcgctcgg cgggcttgcg
     2581 gcgggaaaaa acgcgggaaa tgaacttgcg gatcatcggg tcatcgagtt gtcggcgccg
     2641 ggatcgcgcc gctgggcttg ctgccgcgac cgcttacctg agatgggtgg gcggtcggaa
     2701 cgaggccggc atgataacgc taaccggacg ctatctgagc gacatcgttc gccagccgtg
     2761 ggcactggcg tgggcggcga gcgtcggatc ggcatcgacg gcaaccggat cggagacgcg
     2821 cgcgagcagc ggcaggtcgt tgagcgaatc gctgtagaag ctggtcttgg cgaaactgcc
     2881 ccaccacagg ccgtgtgctt cgagccaggc atcgacgcgg gtcaccttgc cttcgcgaaa
     2941 cgagggtgtg ccggccggtt tgccggtaaa gcggccgtcg acctgcgccg ggatcgtcgc
     3001 aatcaggtgg gcgatgccaa aggcgcgggc aatcggcccg gtgacaaagc tgttggtcgc
     3061 cgtcaccagc gcgcagaggt cgccgcgact gcgatgcccg tcgaccagat ggcgcgcctt
     3121 ctcgccgatg atcggggcaa tgcgttcggc catgaattcg cggtgccagg cgtcgagctg
     3181 cgcgcgcgga tggcgcgcca gtggcgccag ctggaagtcg aggaaggcat ggatgtcgag
     3241 ggtgccggcc ttgtaggctt cgaagaaagc taggtttttc gcttcgtgaa cctcccggtc
     3301 gagcacgccg cgagcgatca ggaactgggc ccattcgaag tcgctgtcgc cgtcgagcag
     3361 cgtgttgtcg agatcgaaga gggcgagatt gagcggcgcg gctggggtcg tcatcggtta
     3421 acggttagcg gttcgggatt cggttcggat tcgagcaggc ggcgcagcag cggcagcgtt
     3481 accgggcggc gatgttcgag cgtgtagcga tcgagcgcat caatcagcgc ggtcagcgtg
     3541 ccgagatcgc gggcgctgcg actgaacagg aagtcgagcg tcccggccgg caattcgagg
     3601 ccgagcgcct cggcgcgcgc cagcaatgcc gccgacttct cggcatcgga cagtgcgttg
     3661 aggcggtaga ccagccccga gccgagccgt gtgcgcaggt cctcgcggat cgccagcgcc
     3721 gccggtggct gcacgctgct gagcagcagc ttgccacggt tctgccggcg gtgattgaag
     3781 gcgacgaaga gcgcagtctg cccggctgcg tcgaggcgat cggcattggc gacggcgagc
     3841 gccggcgccg tctgcgcggc aagcgccgcg gcaacatcga gttcggtggc ggccgcatcg
     3901 acgagcggca ggccgctggc ggcgagcaga tggcggcggc cgctgtgggc ttcgccccag
     3961 agcaggaagc aggtcgggct gccggggagg ctcagccatt cgcgcagcgc ggcgagtgct
     4021 tctgcgttgc cgccaacgat gaagttgtcg aagttcggcg ccgggggcgg cagcaggtcg
     4081 agcaacagtt gccggcccgg ctgcgcttcg gtggttcctg ccgcggtgct catgggcggg
     4141 cgctcattgg cgtgaaaaga aatcgctggc gcgataggcg gcgagcgcgt ggcgcagcgc
     4201 gaccagcagc acggcgctga ccggtagcgc gatgaccacg ccgaagaaac cgagcaactg
     4261 gccgaagacc agcagcgcca ggatgacgac caccgggtgc agcccgatcc gctcgccgac
     4321 cagatatggc gtaagaaaga agctctcgat cagctggccg aggccgaaga tcgcgccgac
     4381 ccagacgaag ggctgccagc cctcgttttg cagaaaggcg gagagcagcg ccaggatcag
     4441 gccgaggccg aagccgacgt aggggatgaa gcagagcagg ccggtgatca gccccagcgc
     4501 cagtgcgtac tcgatgccgg cgacggccag cgcgatgctg tagaaggtgg ccagcgccag
     4561 catcaccgac agctggccgc gcatgaactg gccgagcacg tggtcgattt cgccggtgac
     4621 ctcggaaatg cgcgcctgcc agcgtttcgg tgccagtgac tgggcggcaa aagtgattcg
     4681 cggccagtcg atcagcaggt accaggtgac caccggcacc agcagcagca ggccgagcag
     4741 gcccgctact gcggcaccgc gctgcgacag cgcgacgaac gctttcgccg ctgcaccgcg
     4801 caggctctcg gcgtgctgcc gggcgaggtc agcgaggctg gccagatcga gcggaatcgc
     4861 gccgccgagt ttttcattga gccagggcag gccgcgctca ccgaaggcac gcagcaagac
     4921 cggcagccga tccgctaagc gggcgctttg ttcgatgaac agcggtacga cgaccagcag
     4981 gaagccgagg aaggcgagca gcagcagcac cgtggtcagc acgctgccga aggcgcggcc
     5041 gatgcccagc gcttccagtc gctcgaccag tggattgcag atgtaggcca gcatcgcagc
     5101 cgcggcgaag ggttcgagca ccggggccag cgccaccagc agccagagca gggccgcgac
     5161 gacgacgacc gggatgatct ggcgcaggag cagggtgccg tccggcgcgc cgccggcggc
     5221 cgattgcacc gctgccgccg gactactggt ggcgtcagcg cgggcggtgc cgctgtcggg
     5281 gctggcttgc gcaggctgct cgggtccgtg ggccatggtc atttgcgata gaattgcgcg
     5341 ccatttggcg ccggaacggg ccgcatcgtg cgctgacgcg cggcgctcgc ctcagggtct
     5401 tccggccgcg aattgtagcc cgcgcctcgc gtcgctgtcc gctgccagcc cggcggcatt
     5461 caaccggtgt atccgacatg agcattccag ccactcccct ttcctatcgt gacgccggtg
     5521 tcgacatcga cgccggtgac gccctcgtcg accgaatcaa gccgctggcg aagaaaacca
     5581 tgcgcgacgg cgtgctcgcc ggcatcggcg gtttcggcgc gctgttcgaa gtgccgaagc
     5641 gctaccggga gccggtgctg gtctccggta ccgacggcgt cggcaccaag ctcaagctcg
     5701 ccttccagct caaacggcac gacaccgttg gccaggacct ggtggcgatg agcgtcaacg
     5761 acatcctcgt ccagggcgcc gaatcgctgt tcttcctcga ctacttcgcc tgcggcaagc
     5821 tcgatgtcga taccgccgcc gcggtcgtcg gcggcattgc cagaggttgc gaactcgccg
     5881 gctgcgcgct gatcggtggc gagacggcgg agatgccgag catgtacccg gacggcgagt
     5941 acgacctggc cggctttgct gtcggcgtcg tcgagaaagc caagatcatc gacggccgga
     6001 cgatcgtacc cggtgacgtc gtgctcgggc tg
//
LOCUS       DATDAA010000343         2149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_514692,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000343 DATDAA010000000
VERSION     DATDAA010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514692"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VIS73_10325"
     CDS             complement(<1..888)
                     /locus_tag="VIS73_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY5763588.1"
                     /translation="MEQRFMSNSPSGWRRLVLPAIASMAGVEAFAQSAGAAPVLDAEA
                     REHVAATAAHHLCSGLFVVGRDLSRPVEDVLANDIARFPQFRWSEDYEFRIDFDERSA
                     TVRAPGVLPAQKARYHGDQGCTILPAGVDEPFFEPVEVVSTLPDPATQPWPLGDAAAL
                     GTFPEIDGQKLASAFDRAMSREEHNTRALVVAYRGKIVGERYAEGFGPDTPQIGWSMG
                     KSVTAALIGVLAGAGELDLNEPAPIDEWRGAGDPRGAIRLRDLLNMSSGLEFQNYDDG
                     DRVDAWSASNEHSRIYFDAV"
     gene            913..1239
                     /locus_tag="VIS73_10330"
     CDS             913..1239
                     /locus_tag="VIS73_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763589.1"
                     /translation="MPLSLVVQSARIGASGASSPKSQFHPATSYNGRSIRLEWNTAMR
                     QIALTCGILIVLLIATVGCLYIFEVMTLESAGSAVTKYGSAIVLLGACAAAITFLLGR
                     RKDHQE"
     gene            complement(1250..>2149)
                     /locus_tag="VIS73_10335"
     CDS             complement(1250..>2149)
                     /locus_tag="VIS73_10335"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEY5763590.1"
                     /translation="RDDGRRSRGTGRNRPPGGVKRQLARTKSTKRRNDMTRSLLIALI
                     LAALAGSPTRAQTPDTVFLEELTWTEVRDAIDSGKTTVIVPTAGTEQNGPHMVLGKHK
                     YRMNWGAERIARELGNALVAPVMTYVPEGQIDPPGGHMRYAGTISIPPDVFKAVLEAT
                     ARSLAVHGFTDIILIGDSGPNQQPMRDVADMLNAEWADGEVRLYHVSDWYTSAAFEDW
                     LLEKGETPETIGTHAGLVDTALLLAVAPEHVRMDIAAAGKGRDVDGVTGDPTGATAEL
                     GRTGLEFYVEAAMAQIRSLMADR"
BASE COUNT          321 a          739 c          690 g          399 t
ORIGIN      
        1 gaccgcgtcg aaatagatcc gcgaatgctc gttcgaggcg ctccaggcgt cgacgcggtc
       61 gccgtcatcg tagttctgga attcgagccc gctgctcatg ttcaagagat cgcgcagcct
      121 tatggcgcct cgcggatcgc cggcgccccg ccactcgtcg atgggcgccg gttcgttaag
      181 gtccagctcg cccgctccgg cgagcacgcc gatgagcgcg gccgtgacgc tcttgcccat
      241 ggaccagccg atctgcggcg tgtcggggcc gaagccctcc gcgtagcgct cgccgacgat
      301 cttcccgcgg taggcgacca cgagcgcccg cgtgttgtgt tcctcccggg acatcgcccg
      361 gtcgaaggca gaggcgagct tctggccgtc aatttcggga aaagtgccga gggcggcggc
      421 gtcgccgagc ggccagggtt gtgtggcggg gtccggcagc gtggaaacca cctcgaccgg
      481 ttcgaagaac ggttcgtcga cgcccgccgg caggatcgtg cacccctggt cgccgtggta
      541 gcgggccttc tgcgccggca ggacgccggg cgcccgcacc gtggcgctgc gttcgtcgaa
      601 atcgatccgg aattcgtaat cttcgctcca cctgaactgc gggaagcgcg caatgtcgtt
      661 cgccaggaca tcctcgaccg ggcgcgaaag gtcccggccg acgacaaaca gcccggagca
      721 caagtgatgc gccgccgtcg cggcgacgtg ctcgcgcgct tccgcgtcca gcaccggcgc
      781 ggcgcccgca gactgcgcaa aggcttcaac gcccgccatg ctcgcgatcg ccggcagcac
      841 gaggcggcgc catccggagg gtgaattgct catgaagcgc tgctccatcg gcggcacggt
      901 gttcctaggt taatgccttt atcgctggtc gttcaatcgg cccggattgg cgcctccggc
      961 gcctcgtccc cgaaaagcca attccatcct gcgacgagct acaatggccg ttcaatccgc
     1021 ctcgaatgga ataccgccat gcgccagatc gcattgacct gcggcatcct gatcgtttta
     1081 cttatcgcca cggtcggctg cctctacata ttcgaagtca tgaccctgga aagcgccggc
     1141 tccgccgtga cgaaatacgg ttcggccatc gttttgctgg gcgcctgtgc ggcggccatt
     1201 accttcctgc tggggcgccg caaggatcac caggagtagg cgcagccatt cagcgatcgg
     1261 ccatcagcga ccggatctgc gccatcgccg cttcgacgta gaattccagg cctgtcctgc
     1321 cgagctccgc cgtcgccccg gtcgggtcgc cggtgacgcc gtcgacgtcc cggcccttgc
     1381 cggcggccgc aatgtccatg cgcacgtgct cgggagcgac ggccaggagc agcgccgtgt
     1441 ccaccagccc ggcatgcgtc cctatcgttt cgggagtctc gcccttttcg agcagccagt
     1501 cctcgaacgc cgccgacgtg taccagtccg atacgtggta caggcggacc tccccgtccg
     1561 cccattccgc gttcagcatg tcggcgacgt cccgcatcgg ttgctggttc ggaccgctgt
     1621 cgccgatcag gatgatgtcg gtgaagccgt ggacggccag gctccgcgcc gtcgcctcca
     1681 ggaccgcctt gaaaacatcc ggcggaatcg aaatcgtgcc tgcatagcgc atgtgcccgc
     1741 ccggcggatc gatctgccct tcgggcacgt aggtcatcac cggcgccacc agcgcattgc
     1801 ccagctcgcg ggcgatgcgc tcggcgcccc agttcatgcg gtacttgtgc ttgccgagaa
     1861 ccatgtgcgg cccgttctgc tcggtgccgg ccgtcggcac gatcaccgtc gtcttgccgc
     1921 tgtcgatggc gtcgcggacc tcggtccagg tcagttcctc gaggaagacg gtgtccggcg
     1981 tctgcgcccg agtgggcgat ccggcaaggg cagcgagtat cagggcgatc aggagggatc
     2041 tcgtcatgtc gtttcttcgc ttggtggatt tcgttcgggc gagttgccgt ttcactccgc
     2101 cgggcggccg atttcggcca gtgccgcgac ttcttctgcc gtcatctct
//
LOCUS       DATDAA010000344         3165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_514704,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000344 DATDAA010000000
VERSION     DATDAA010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514704"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..587
                     /locus_tag="VIS73_10340"
     CDS             45..587
                     /locus_tag="VIS73_10340"
                     /inference="COORDINATES: protein motif:HMM:NF019448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763591.1"
                     /translation="MRYLANESRDPRLDEVVMALCAEMLVASGLESARDAARSRADAA
                     LTSGKAAEVFERMVVGLGGPPDFVTGHEQHLPVASLQRAVYPDTSGFLSTVDAFRLGQ
                     AVIGLGGGRRKLGDTLNLAVGLAEVAPIGARVDHERPLAVVHANSVPAAKKAAALVLE
                     ACEISDSEPATGDVIHDLLN"
     gene            679..1905
                     /locus_tag="VIS73_10345"
     CDS             679..1905
                     /locus_tag="VIS73_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEY5763592.1"
                     /translation="MSGKLIGLVGIAVILGIAYLISSNRKAINIRIVGAAFALQFALA
                     VFIIKVPAGQAVIESMSNVVLGIMGYSRVGIEFIFGGLATESSVASFAVNVLPMIVFF
                     SALMSVLYHIGIMQLIVRLVGGALRWIIGTRAIESLNAAANIFIGQTEAPLVVRPYLK
                     DLTEPQMFAVMVSGVASIAGTVLLAYVQFGAELKYLLAASFMAAPGGLLMAKIIMPDE
                     QAESAAEDDAHLKLGKSPHENVILAAAVGSADGVRLAVNIGAMLVAFIALIALLNGLL
                     GALAGLVGIESLTLQQIFGWLFSPLMYLLSIPWNEAQEAGALFGEKIILNEFVAYSHL
                     NEYLAGMSERTVAIVTFSLCGFANLSSIAILLGGLGSLVPEKKELIARFGLKAVAAGS
                     LSNLMSAALAGLLLPL"
     gene            1916..2857
                     /locus_tag="VIS73_10350"
     CDS             1916..2857
                     /locus_tag="VIS73_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEY5763593.1"
                     /translation="MSGRPIIIDCDPGQDDAVNLLLALSSPDELDVLAITTVAGNVPL
                     ELTERNTRQICDIAGRTDMPVYAGCDRPMRRPLLTAEKVHGNTGIDGIEIVEPVTPLR
                     DGHAVDFIIEALGAAEAPVTLVPTGPLTNIGTAIEREPAILGSVAEIVLMGGAMREGG
                     NHSPSAEFNILVDPDAADIVFRCGRPVTVFGLDVTHQVLATDERRARIRAIGNPAGQA
                     TAGMLDFFNRHDTAKYGSLGAPLHDPCTVAWLLKPELFEGKPCNLTVETESELTMGHT
                     AVDFWQVTERPHNVTWMHSVDADGFYALLVERLERYG"
     gene            2850..>3165
                     /locus_tag="VIS73_10355"
     CDS             2850..>3165
                     /locus_tag="VIS73_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY5763594.1"
                     /translation="MAEAPSIVVVGSVNLDIVATAERLPLAGETVTGAALDRFPGGKG
                     ANQALAARRLGARVALVAAVGDDADAGQALALLRADGVDLSQCSVEPAAPTGVALIAV
                     APS"
BASE COUNT          495 a         1067 c         1068 g          535 t
ORIGIN      
        1 gtctcggcac gaccgccggc aacgcgctcg aaatagccga agcgatgcgc tacctcgcga
       61 acgaatctcg cgatccgcgg ctcgacgagg tcgtcatggc gctgtgcgcc gagatgctgg
      121 tcgcgtcggg cctcgaatcc gctcgcgacg cggcgcgatc ccgggcggac gcggcgctga
      181 cgtcagggaa ggcggccgag gtcttcgagc ggatggtcgt cgggctcggc ggcccgccgg
      241 atttcgtcac cggtcacgag cagcacctgc cggtcgccag cctgcagcgg gcggtctatc
      301 ccgacacgtc gggcttcctg tccaccgtcg atgcgttcag gctgggccag gctgtgatcg
      361 ggctcggcgg cgggcgccgg aagctcggtg atacgctgaa ccttgccgtc ggccttgccg
      421 aggtcgcgcc gatcggcgcc agggtcgatc acgagcggcc gctggccgtg gtccacgcga
      481 attcagtccc ggccgccaaa aaggcagcgg cgctcgtgct cgaggcctgc gagatcagcg
      541 attccgagcc tgcgacgggc gacgttatcc acgacttgct gaactgatat tggtagggaa
      601 aagcggccac ggagccggta tccttgctcg gcaggacctg ccaataacaa taaatccgct
      661 tcagccaggg ggccacctat gtcgggcaaa ctgatcgggc tcgtgggcat cgccgtgatt
      721 ctcggcatcg cctacctgat ttcgagcaac cgcaaggcga tcaatatccg catcgtcggc
      781 gcggccttcg cgctgcagtt cgccctcgcg gtattcatca tcaaggtccc ggcgggccag
      841 gcggtgatcg agtcgatgag caatgtcgtg ctcgggatca tgggctattc gcgcgttggc
      901 atagagttca ttttcggcgg cctggcgacg gaaagcagcg tcgccagttt tgcggtcaac
      961 gtgctgccga tgatcgtctt cttttccgca ttgatgtcgg tcctctatca catcggcatc
     1021 atgcagctga tcgtcaggct ggtcggcggg gccttgcggt ggattatagg gacccgggcg
     1081 atcgagtcgt tgaatgccgc cgccaacata ttcatcggcc agaccgaggc gccgctggtc
     1141 gtgcggccgt atttgaagga cctgaccgag ccgcagatgt tcgcggtcat ggtgtccggc
     1201 gtggcgtcga tcgccggcac cgtcctgctg gcctacgtgc agttcggcgc cgagctcaag
     1261 tacctgctgg cggcgagctt catggccgcg cccggcggcc tattgatggc caagatcatc
     1321 atgccggacg agcaggcgga gtcggctgcc gaggacgacg cccacctgaa gctcggcaag
     1381 agcccgcacg agaacgtgat cctggccgcc gcggtgggtt cggccgacgg cgtcaggctg
     1441 gcggtcaata tcggcgcgat gctggtcgcc ttcatcgcgc tgattgccct tctcaacggc
     1501 ctgctcggcg cgcttgccgg cctcgtcggc atcgaaagcc tgacgctgca gcagatcttc
     1561 ggctggctgt tctcgccgct gatgtacctc ttgagcattc cctggaacga ggcgcaggag
     1621 gccggcgcgc tgttcggcga aaagatcatc ctcaacgagt tcgtcgccta cagccacctc
     1681 aacgagtacc tcgccggaat gagcgagcgc accgtcgcca tcgtcacctt ctcgctgtgc
     1741 ggtttcgcca acctgtcctc catcgcaatt ctgctgggcg gtctcggcag cctcgtgccc
     1801 gagaagaagg aactcatcgc acgcttcggc ctcaaggccg tggccgccgg ctcgctgtcc
     1861 aacctgatga gcgcggcgct cgcggggctg ctgttgccgc tgtgaggcac agcggatgag
     1921 cggccgcccg atcatcatcg actgcgaccc ggggcaggac gacgccgtca acctgctgct
     1981 ggcgctgtcg tcgcccgacg agctggacgt gctcgccatc accacagtgg ccggcaacgt
     2041 gccgctcgaa ctcaccgaac gcaatacccg gcagatctgc gacatcgcgg gccgcacgga
     2101 catgccggtg tacgccggct gcgaccggcc catgcgccga ccgctgctta ccgccgaaaa
     2161 agtgcacggc aataccggca tcgacggcat cgaaatcgtc gagccggtga cgccgctccg
     2221 cgacgggcac gcggtcgact tcatcatcga ggcgctggga gcggcggagg cgccggtcac
     2281 gctggtgccc acgggaccgc tcaccaacat cggcacggcg atcgagcgcg agcccgcgat
     2341 tctcggttcg gtggccgaga tcgtgctgat gggcggcgcg atgcgcgagg gcggcaatca
     2401 ctcgccgtcg gccgagttca atatcctcgt cgatcccgac gccgcggata tcgtgtttcg
     2461 ctgcggccgg cccgtcaccg tcttcgggct cgacgtgacc caccaggtcc tggcgaccga
     2521 tgagcgccgt gcgcgcatcc gggcgatcgg caacccggcc ggccaggcga cggcgggcat
     2581 gctggacttc ttcaaccgcc acgacacggc caagtacggg tcgctcggcg cgcccctgca
     2641 cgatccctgc acggtcgcgt ggctgctgaa gccggagctc ttcgaaggca aaccgtgcaa
     2701 cctcaccgtc gagacggagt cggagctcac gatgggacac accgccgtcg acttctggca
     2761 ggtcaccgag cggccgcaca acgtcacctg gatgcacagc gtcgacgccg acgggttcta
     2821 cgcgctgctg gtcgaaaggc tcgagcgcta tggctgaagc tccctcgatc gtcgtcgtag
     2881 gctcggtgaa tctcgacatc gtcgccacgg cggagcgcct gccgctggcc ggcgagaccg
     2941 tgaccggcgc cgcgctcgac cgctttcccg gcggcaaggg cgccaaccag gcgctggccg
     3001 cgcgccgcct gggggcccgg gtcgcgctgg tcgccgcggt gggcgacgac gccgatgccg
     3061 gccaggcgct cgcgctcctg cgagcggacg gggtcgacct gtcgcaatgc agcgtcgagc
     3121 cggcggcgcc gaccggcgtc gcgctgatcg cggtggcgcc ctccg
//
LOCUS       DATDAA010000345        19610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_200247,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000345 DATDAA010000000
VERSION     DATDAA010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 19610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19610
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200247"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2119)
                     /locus_tag="VIS73_10360"
     CDS             complement(<1..2119)
                     /locus_tag="VIS73_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY5763595.1"
                     /translation="MAFKLKLPSFGKKKDSSTTTVAPATVIDKTILPSTAKKPEGGHG
                     GVLMLSRFPIAKQLQMLGVVVAILIMLAVGLIYRDNRENAYTQAYTNNAHQMRLLSQV
                     LARVGVQAGQGRRAAFRELKLSREQFTQLLSVLAVGGEQAGMSLPPVPDQVRGQIDVI
                     SAQWRKSDEDAAALLDQETSLVSLANSVSEINKKNEESTRLLDQFVAQRTTLGSARDV
                     SYANQMQVLSLRIAKNANALLASDRFPPELAFAIDRDVKGLREILDGFADGSGPLRIS
                     AVGDAAGKKLLAEVDGLFAEDFKVISGVLADMQKLVRAKEASARLTSESEALLAASTK
                     LEETFVDSSARTTFFIALFVIALLIVTVIALMAKVFFDDVHHRATESDRQRKESESTN
                     RSNQDAILRLMNELGDLADGDLTVTATVSEDITGAIADSINYTIDELRVLVGRINDAA
                     TRVSTTTESAQKTSARLLAAAELQSREIQNAGHSALEMARSMNQVSSNANQSAQVARQ
                     SLAAAEKGTEAVEDSIKGMNDIRDQIQETSKRIKRLGESSQEIGEIVELISDITEQTN
                     VLALNAAIQAASAGDAGRGFTVVAEEVQRLAERSGEATKQIAAIVKTIQTDTQDAVSA
                     MEQSTQGVVEGAKRSDAAGQALSEIGRVSRDLAGLIETISSATQTQSKSATSVAEAMQ
                     RILHITEQTTAGTQKTAAAVGELA"
     gene            complement(2165..2677)
                     /locus_tag="VIS73_10365"
     CDS             complement(2165..2677)
                     /locus_tag="VIS73_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY5763596.1"
                     /translation="MAKKLSLREFQEYLAKRLTSAARGEISSALLGVQSGKESWLLNL
                     ADAGEIIPVAAMTAVPLTHPWFCGVANIRGNLYSVVDFSAFRGAEPTPINANARLVLI
                     GTRFNVNCALLVSRMLGLKPGDAFKPAAADGELPGWIAGDLEDNEGRRWRHLDVPGLI
                     ATPGFMQVGL"
     gene            complement(2700..3062)
                     /locus_tag="VIS73_10370"
     CDS             complement(2700..3062)
                     /locus_tag="VIS73_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5763597.1"
                     /translation="MPVKKILVVDDSPTERHYMVELLTKNGYVVTTAENGEEGIAKAK
                     AEHPDLVLMDVVMPGLNGYQATRTLTRDEATKDIPVIVCTSKGQETDRIWCLRQGAHD
                     YMVKPVNADELLKKIAAL"
     gene            complement(3073..3435)
                     /gene="pilG"
                     /locus_tag="VIS73_10375"
     CDS             complement(3073..3435)
                     /gene="pilG"
                     /locus_tag="VIS73_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HEY5763598.1"
                     /translation="MVIDDSNTIRRSAEIFLSKAGCQVILAEDGFDALAKIADSQPDV
                     IFCDIMMPRLDGYQTCALIKKNPRFSGTPVVMLSSKDGLFDRARGRMVGSDEYLTKPF
                     TKDSLLQAVAQHAGAAQA"
     gene            3428..3559
                     /locus_tag="VIS73_10380"
     CDS             3428..3559
                     /locus_tag="VIS73_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763599.1"
                     /translation="MTITLRPRSSFSSANYEVSGIRVASGLGLWETREILTVLAGVA"
     gene            3725..4378
                     /gene="thiE"
                     /locus_tag="VIS73_10385"
     CDS             3725..4378
                     /gene="thiE"
                     /locus_tag="VIS73_10385"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY5763600.1"
                     /translation="MPVQRPFPIRGLYAVTPESVDRAQLLAFAKQALAGGAVLLQYRD
                     KSADSAERLARAAELRQLCSAAGVPLIVNDAVDLALAVGADGVHLGADDGDPAAARSR
                     LGDGAIVGVSCYASLERAERALAAGADYLAFGAAFASPTKPAAVPVSLPTIAEACRRF
                     PARVAVIGGITAENAVPLVAAGVSLLAVITDLCAAPDLMARARQYQSLFTGSGHDHA"
     gene            4365..5645
                     /gene="hemL"
                     /locus_tag="VIS73_10390"
     CDS             4365..5645
                     /gene="hemL"
                     /locus_tag="VIS73_10390"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY5763601.1"
                     /translation="MTTRNEMLFQKAQRHIPGGVNSPVRAFGSVGGTPRFFAEARGAY
                     AWDADGKKYIDYVGSWGPAIVGHAHPEVLKAVIAAAEKGLSFGAPTEQEIVLADALCD
                     HLPGMEMIRLVSSGTEATMSAIRLARGFTGRDGLIKFEGCYHGHSDSLLVKAGSGALT
                     FGNPSSGGVPADLAKHTMVLDYNDIDQLEGAFARHADQIACVIVEPVAGNMNLIAPKP
                     GFLKAMRELCTQHGAVLIFDEVMTGFRVGPKCAQGLYGIVPDLTTLGKVIGGGMPLGA
                     FGGRRDIMEKIAPLGPVYQAGTLSGNPVAVAAGLATLKIVLADGFYEALTAKTKRFAE
                     GMATAGRDAGRAFSAQSVGGMFGLYFSATPPTSYREMMAGDREAFGRFFHAMLDEGVY
                     LAPAAFEAGFVSAAHSDADIDATVAAAKRAFAKI"
     gene            complement(5664..7106)
                     /gene="mgtE"
                     /locus_tag="VIS73_10395"
     CDS             complement(5664..7106)
                     /gene="mgtE"
                     /locus_tag="VIS73_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY5763602.1"
                     /translation="MDAEIEHADSEELQHRLGEVQRLLARQKVVEDLVERQEMQRHEL
                     VEDLVHRQNLAELKARLDRMHPADIAFILEALPLDDRMIVWDLVKAEHDGEILLEVSD
                     AVRECLIETMDTREIVAVAEQLDADELADLVPDLPEEVIADVFQALPAEEREQLRAAM
                     SYPEGSVGALMDFEMVTVREDVTVEVVLRYLRRLRELPDHTDKLFVVDRDNRLRGVLP
                     INAILVSDPEVEVSSLMDNDFISFEPIDEAEDASQSFERYDLVTAPVVDADERVIGRL
                     TLTAVMDYVVEENETEILNQAGLREEEDIFAPVWDSVKNRWAWLAVNLVTAFVASRVI
                     GAFEGSIEKLVALAALMPIVAGIGGNSGNQTITMIVRAIATGQVEPSAMQRLLKKELG
                     VALFNGVVWGGLLGLIAWWLYGSAALGVVMTAAMLLNLLLAASAGVLVPMLRHRLGAD
                     PAVGGSVLITALTDSGGFFIFLGLATLFLL"
     gene            complement(7204..7875)
                     /gene="mtgA"
                     /locus_tag="VIS73_10400"
     CDS             complement(7204..7875)
                     /gene="mtgA"
                     /locus_tag="VIS73_10400"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEY5763603.1"
                     /translation="MLLLVGIVLVVLAYQVWLFGHVLWWSRHDPESTRFMDIRLAELR
                     VRKPEAEIKQRWVPYARISNHLKRAIVAAEDAKFVDHEGFDWEGIQRAIEKNQQKGRA
                     VAGGSTISQQLAKNLFLTPQRSYWRKAEEALITLMIEATMDKQRILEIYLNVVEWGNG
                     VFGAEAAARHYFGVSAAGLDAWQAAWLAGALPNPRYYDRNRGAPGLARKTLIIVERMP
                     DAAIP"
     gene            complement(7928..8782)
                     /gene="aroE"
                     /locus_tag="VIS73_10405"
     CDS             complement(7928..8782)
                     /gene="aroE"
                     /locus_tag="VIS73_10405"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY5763604.1"
                     /translation="MTDRYAVLGNPIAHSKSPAIHAQFAAQCGEDMHYEAILAPIDGF
                     AATLGGFLAAGGRGANVTVPFKEEAWRLADRRTPRAEAAGAVNTLAVSAEGLLGDNTD
                     GAGLVRDLRANLGQQLTGCRVLLLGAGGAARGVLAPLLAENPAEILIANRTPERAQAL
                     AAQAGDRSRVAGIGFAELPARGFDVVINATSASLAGAVPPVAACHFGAGALAYDMMYG
                     AGDTAFLAFVRGLARPDGPPLRFADGLGMLVEQAAEAFALWRGKRPDTAPVFAALRAD
                     LATPASRR"
     gene            complement(8795..9682)
                     /locus_tag="VIS73_10410"
     CDS             complement(8795..9682)
                     /locus_tag="VIS73_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY5763605.1"
                     /translation="MVAAIASSMRNAYGADRRFAWALGASLAAHALILSIHFGLPSAE
                     RIFRERPLDVVLVNAKTTRKPQKAEVLAQSNLDGGGNTDESRRARTPLPASTQHIAGD
                     KLEAAQQRVFQLEKLQRDLLAQIKREAPKVNAGKTKNAQPQETPPTVSGRDLADNALA
                     MARLQAEIDRNLEAYAKRPRRAFVGARAAEYRFARYVEDWRLKVERIGTLNYPPAARG
                     KLYGNLIMTVIVRADGQVEAVEVNRSSGHKVLDDAARRIVQMAAPFAPFPSDIRRDTD
                     IIEITRTWFFTRDDALRSQ"
     gene            complement(9685..11610)
                     /locus_tag="VIS73_10415"
     CDS             complement(9685..11610)
                     /locus_tag="VIS73_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEY5763606.1"
                     /translation="MHLLFEEDGSFRGGTILADNTTSLQVETATGKRAKIKRAQVLLE
                     FGDPAPGELLPAAEPLAAGIDAAFLWECCGDGEFAFTDFANDYYGHRPSAVEAAAVLL
                     ALHAAPIHFHRKGKGRFRKAPPDILAAALAGQEKKRQQAETVERLAAQLAGGDLPAEI
                     AQAMPAILYRPDRNRLETKALEAACEQAQATPAQLLLRCGALHSPYDFHLGGFLFANF
                     PDASEVPAPPSGSFGAELPRAEAAAFSIDDVQTTEIDDAFSVQALPGIGWRIGIHIAA
                     PALAFTPDSPAGAQARARLSTVYMPGHKITMLPDDWVGSCTLIEGRDAPAVSLYLTVS
                     EQFEILGHESRIERVAIAANLRLHELEPLFNEQTLTNGLGEFRFARELETLWRLALAC
                     ESRRGKPAAKQGIFDFHFRVDGDVSSPGADLSNCRIHIEERRRGSPIDLLVSELMIVA
                     NSTWGGLLDEHRVAGLYRTQTSGKVRMSTQAAPHEGLGVAQYAWCTSPLRRYADLLNQ
                     WQLVACLRDDNPPFANRSEALFGALRDFEIAYASYAEFQRQLERFWCLRWLAQEGLVG
                     DTPLRATAMRREGQFRLDHLPLVLNVSSAPALTPGTAILLRIASIDELALGVDARHLE
                     TLAEHSGADDDAVEEIA"
     gene            complement(11617..12204)
                     /locus_tag="VIS73_10420"
     CDS             complement(11617..12204)
                     /locus_tag="VIS73_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEY5763607.1"
                     /translation="MIVYLHGFCSSPASAKARQLADYLARLGRANEFRCPALPPDPVQ
                     AIAAIEATLADLPSPATLIGSSLGGFYSTCLAERHGLQAVLVNPALPTHLETVPLIGE
                     HRNFHSGEAFTFTAAHQQALLDMAPRHLTPNRYLLLVETGDEVLDAQVTTTALAGARQ
                     VVLAGGDHSFTRFTEFLPTIVDYADAARNAAQPQI"
     gene            complement(12201..13019)
                     /locus_tag="VIS73_10425"
     CDS             complement(12201..13019)
                     /locus_tag="VIS73_10425"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY5763608.1"
                     /translation="MDALLAALLLGIVEGVTEFLPVSSTGHLIVVGDLVGYNDETSKV
                     FKIVIQLAAILAVCWDYRERLTKVAMGFGREPAANRFVLLLFVGFLPAAVLGLLFHGA
                     IKALLFNPITVATALVVGGFLILLIERRADSARIHEVDDMGWRDALKVGFAQALAMIP
                     GTSRSGATIMGGLVFGLSRKTAAEFSFFLAIPTMFAATAYDLYKNWALLSADDLPIFA
                     VGFVASFVSAMWAVKGFIRYVAHHSFAVFAWYRIAFGLLVLASWQLGWVDWSTT"
     gene            13127..13816
                     /locus_tag="VIS73_10430"
     CDS             13127..13816
                     /locus_tag="VIS73_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763609.1"
                     /translation="MTKKICKCLLIVGFAVGVSAVHAQGLRAPAAGLASDGLLDRYWE
                     SGGLKSFMQTLPKTERESGPLLGGWSYAVGEDSGFSVGLGWQPGETTEDIGYLGAAGA
                     SWRQRFYGFWDPVITAGLSIMRQPHQESGLVGQLYGAHVSLGLNPSSDWGISLGLGYR
                     DAMDANPLATAAERADQRAVYGDLAAVYRLQRNLELQGQITGLDLRSRSLAANPDSTR
                     EVGIRMRYFFD"
     gene            complement(13801..14757)
                     /locus_tag="VIS73_10435"
     CDS             complement(13801..14757)
                     /locus_tag="VIS73_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763610.1"
                     /translation="MLSNTRALDVTGTVRTDDPSAVARACIAIQQHRYGKDAVDSQFL
                     FRAFEEARRAYFGESPDWLACDTPYHDLRHALDTALCATRLADGFDIAAKSAEQRLGA
                     RLAATTTVLALLHDTGFLRSRHQEDGQDGARFMAEHEARSVAWVRASPLAAAWPELAA
                     CAELIQATNIAADALASAAVADQRRAAMANIIGTADFLAQIADRYYLEKCRDFLYTEF
                     VRAGMDRRQEADGSTTVLYLSGEDLLRQTPGFFASFGPQRLDQRFARCHTCLDVHFGG
                     RNPYLEAASSNLARLEKLLATGRLGELRRRPRALPPSAQSKK"
     gene            complement(14776..15381)
                     /gene="gstA"
                     /locus_tag="VIS73_10440"
     CDS             complement(14776..15381)
                     /gene="gstA"
                     /locus_tag="VIS73_10440"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEY5763611.1"
                     /translation="MKLYYSPGACSLSPHIVLAETGLPYTLERVDLKTRKTEAGIDFS
                     TINSKGYVPVLRLDDGAFLTEGPAIVQYIADRAPDSGLAPAAGTLPRYRLQEWLSFIG
                     TELHKTFSPLWHSQNDEIRQAASTALERRFDWLTPQLVKRPFLMGDRFTVADAYLFTV
                     LNWTSFLKIPLDRWQALSAYLAQIAARPAVLRTLDEEGLRR"
     gene            complement(15505..16614)
                     /locus_tag="VIS73_10445"
     CDS             complement(15505..16614)
                     /locus_tag="VIS73_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEY5763612.1"
                     /translation="MSAQMPSALFSPIALRGLTLANRIVIAPMCQYSAVDGLIDDWHL
                     IHLGSLAHSGAGLLIVEATAVTPEGRISDRCPGLWSDAHEAAFARVLAGVRRHSAMPL
                     AIQLAHAGRKASCAAPWLGGRQLALDAGGWNTLAPSPLAFRDADRVPLELDAAGLARI
                     RQSFVDAAQRADRLGFDGIELHGAHGYLLHQFLSPLANRRTDDYGGSLANRLRFPLEI
                     FDAVRAVWPERKPLGMRVSATDWVEGGWDVAQTAALGHELKARGADYIHVSSGGLSPA
                     QQIVAGPGYQVPFARTIRAETRLTTIAVGEITRAQQAEEILGSGDADLVALARGILYD
                     PRWPWHAAAELGAQVDAPPQYWRADLSARGRLFRD"
     gene            complement(16622..18538)
                     /gene="thiC"
                     /locus_tag="VIS73_10450"
     CDS             complement(16622..18538)
                     /gene="thiC"
                     /locus_tag="VIS73_10450"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEY5763613.1"
                     /translation="MNAKDQFIAATAHVDSAAVEPLPNSRKVYIEGSRPDLRVPMREI
                     SQATTPTGFGGEANPPVFVYDCSGPYSDPAAKIDIRKGLPAMRAQWIAERGDVESLPD
                     LSSQFGRERAADKALDELRFPGLHRKPLRAKAGMNVSQMHYARKGIITPEMEFVAIRE
                     NNNRAAYIESLRATGAQGEKMAKLLTRQHKGQDFGASIPGEITPEFVRSEVARGRAII
                     PNNINHPESEPMIIGRNFLVKINANIGNSALGSSIQEEVEKMTRSIRWGGDTVMDLST
                     GKNIHETREWIIRNSPVPIGTVPIYQALEKVNGRAEDLTWDIFRDTLIEQAEQGVDYF
                     TIHAGVLLRYVPMTANRMTGIVSRGGSIMAKWCLAHHKESFLYTHFEDICEIMKAYDV
                     AFSLGDGLRPGSIYDANDEAQLGELETLGELTDIAWKHDVQTIIEGPGHVPMHLIKEN
                     MDLQLKHCKEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWYGTAMLCYVTPKEHLGL
                     PDKDDVKEGIITYKLAAHAADLAKGHPGAQIRDNALSKARYEFRWDDQFNLGLDPDKA
                     KSFHDETLPKESAKVAHFCSMCGPHFCSMKITQEVREFAAAQGIAETEALTKGMETKA
                     VEFVKTGAELYKKV"
     gene            complement(18587..19444)
                     /gene="thiD"
                     /locus_tag="VIS73_10455"
     CDS             complement(18587..19444)
                     /gene="thiD"
                     /locus_tag="VIS73_10455"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY5763614.1"
                     /translation="MIASATSSASPSRTPNVVSIAGVDPSGGAGIFADLKTFSALGAY
                     GCGVVAALTAQNTRSVTGVHLPPTDFIRLQIDTLFADVAIASAKIGMLGTAGVVRTVA
                     DALLGRPLPYLVLDPVMVAKSGDRLLADDAIAMLREALFPLATMITPNLPEAAVLLKL
                     STPESPKEMRRAAEKLREILPHSGRRWVLLKGGHLPGGDLLDLLFDGDNMIELESRRV
                     ETNNTHGTGCTYSAAIAALLPQRPEVPSAVLDAHRWLARAIERSGDLAVASTPTGHGP
                     VHHFHALWR"
     regulatory      complement(19432..19537)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         3102 a         6175 c         6841 g         3492 t
ORIGIN      
        1 aagcaagttc gccgaccgcg gcggcggtct tttgcgtacc ggcggtcgtc tgttcggtga
       61 tgtggaggat tcgctgcatc gcctcggcga ccgaggtcgc cgatttggac tgggtctgcg
      121 tcgcgctcga aatcgtttcg atcaggccgg ccaagtcacg cgaaacgcgg ccgatctcgg
      181 acagcgcctg accggcggcg tcggatcgct tggccccttc aaccacgccc tgcgtgctct
      241 gttccatggc cgataccgcg tcctgcgtat cggtctgaat cgtcttgacg atcgccgcga
      301 tctgtttagt cgcctcgccc gagcgctcgg ctagtcgctg aacttcctcg gcaacgaccg
      361 tgaagccgcg gcctgcgtcg ccggccgaag cggcctgaat cgccgcgttc aatgccagca
      421 cgttggtctg ttcggtaatg tcggaaatca gttcgacgat ctcgccaatc tcctgcgagg
      481 attcgccaag tcgcttgatc cgtttcgagg tctcctggat ctggtcacga atgtcgttca
      541 tgcccttgat cgagtcttcc accgcctcgg tacccttctc ggcagcggcc agcgactggc
      601 gcgccacctg cgccgactgg ttggcgttgg aggaaacctg gttcatcgaa cgcgccattt
      661 ccagcgccga gtggccggcg ttctgaattt cgcgtgattg cagttcggca gcggcgagca
      721 gacgcgctga ggttttctgc gccgattcgg tcgttgtcga cacgcgtgtt gcggcgtcgt
      781 tgattcgtcc gaccagcacg cggagttcgt caatcgtgta gttgatcgag tctgcgatgg
      841 cgccggtgat gtcctcggaa acggtcgcgg tgacggtcag gtcgccgtcg gccaggtcgc
      901 cgagctcgtt catcagtcgc agaatcgcgt cctggttcga acggttggtg ctctcagatt
      961 ccttgcgctg ccggtcggat tcggtggcgc ggtggtgaac gtcgtcgaag aagaccttgg
     1021 ccatcaaggc gatcactgtg acgatcagca gggcaatcac gaacagcgcg atgaagaagg
     1081 tcgtacgggc actagagtcg acgaaggttt cctcgagctt tgtcgaagcc gcgagtagcg
     1141 cctccgattc gctggtcagc cgggccgagg cctccttggc gcggaccagc ttctgcatgt
     1201 cggcaagcac gccgctaatg accttgaagt cctcggcgaa gaggccgtcg acttctgcca
     1261 gcagcttctt gccggccgcg tcgccgaccg ccgatatgcg cagcgggccg ctgccgtcgg
     1321 cgaagccgtc gagaatctcg cgcaggccct tgacgtctcg gtcgatggcg aaggccagtt
     1381 cgggcgggaa gcgatcgctg gccagcagcg cgttggcgtt cttggcgata cgcagcgaca
     1441 gtacctgcat ctggttggcg tacgacacgt cgcgcgccga tcccagcgtc gtgcgctggg
     1501 cgacgaactg gtcaagcagt cgggtggact cctcgttctt cttgttaatt tcgctgacgg
     1561 aattggccaa gctgaccaga ctggtttcct ggtcgagcag cgctgccgcg tcttcgtcgc
     1621 tcttgcgcca ctgcgcgctg atcacgtcga tctggccgcg gacctggtcg ggtaccggcg
     1681 gcagcgacat gccggcctgc tcgccgccga cagcgagcac gctcagtagc tgggtgaact
     1741 gctcgcgcga cagtttcagt tcgcggaagg cagcgcgacg cccctggccg gcctgaacgc
     1801 cgacccgtgc cagcacctgc gagagcaggc gcatctggtg cgcgttgttg gtgtacgcct
     1861 gggtgtaggc gttttcgcgg ttgtcgcgat agatgaggcc gacggcgagc atgatcagga
     1921 tcgccaccac cacgccgagc atctgcagct gcttggcgat cgggaagcgc gagagcatga
     1981 ggacgccccc gtgtccgccc tccggtttct tggcggtact gggcaggatc gtcttgtcga
     2041 tgacggtcgc cggcgcgacg gtggtcgtgg acgagtcctt cttcttgccg aaggacggca
     2101 gtttcagttt gaacgccatg cttgcctctc ccgggcggtc atttcgtgga tgcccgtttc
     2161 ttgatcaaag tccaacctgc atgaaaccgg gcgttgcgat caggcccggc acgtcgaggt
     2221 gccgccagcg gcggccttca ttgtcttcga ggtcgccggc gatccagccc ggcagttcac
     2281 cgtcggccgc cgccggcttg aacgcgtcgc cgggcttcag gcccagcatc cgcgagacca
     2341 gcagcgcgca gttcacattg aagcgcgtgc cgatcagcac cagtcgtgcg ttggcattga
     2401 tgggcgtcgg ctcggcaccg cggaaggccg agaagtcgac gaccgaatac aggttgccgc
     2461 ggatgttagc gacgccgcag aaccacgggt gcgtcagcgg caccgctgtc atcgccgcca
     2521 ccgggatgat ttcgcctgcg tcggccagat tgagcagcca cgattccttg cccgactgaa
     2581 cgccgagcag cgccgacgag atctcgcctc gcgccgcgct ggtcaggcgc ttggccaggt
     2641 attcctggaa ttcgcggagg ctgagtttct tggccattaa ggaaagggcg cctgctcggt
     2701 cagagcgccg cgatcttctt cagcagttcg tcggcgttga ccggcttcac catgtaatcg
     2761 tgggcgccct ggcgcagaca ccagatgcgg tcggtttcct gtcccttgga ggtacagaca
     2821 atgaccggga tgtccttggt cgcctcgtcg cgcgtcagcg tgcgagtcgc ctggtagcca
     2881 ttgagcccgg gcatgacgac atccatcaga accaggtcgg gatgctcggc cttggccttg
     2941 gctatgcctt cctcgccgtt ctccgcggtg gtgaccacgt aaccgttctt ggtcagcagc
     3001 tcgaccatgt agtggcgttc ggtgggcgag tcgtcgacaa cgagaatttt cttgaccggc
     3061 atgatctttc tcctacgcct gtgccgcgcc tgcgtgctgc gcgacggcct gcaacagact
     3121 gtctttggta aagggtttgg tgaggtactc gtccgagccg accatccggc cgcgggcacg
     3181 atcgaaaagg ccgtccttgg acgacagcat gaccacgggg gtccctgaaa agcggggatt
     3241 cttcttgatc agcgcgcacg tctgatagcc atcgaggcgc ggcatcatga tgtcacagaa
     3301 aatgacgtcg ggctggctgt cggcaatctt cgccagcgca tcgaagccat cctcggcgag
     3361 gatcacctgg catccggctt tcgacagaaa aatctcggca ctgcggcgga tggtgttgga
     3421 atcatcgatg accatcacct tgcggccgcg cagttcgttt tcgtctgcca actatgaagt
     3481 ctccggaatc cgcgtcgcca gcgggcttgg gttatgggaa acgcgcgaaa tactaactgt
     3541 tcttgccggt gttgcctagc aggcgcaaat cgtctagttc ggcccggtgt gacggttttg
     3601 gcggggtcgg cgaatgtcgg cgacgggcct agtccggcaa tcgatgaaac ggtgcatcgg
     3661 ctagaatcgc cgctttcagc cgtcgccgcc tccggctcgg cagccattcg agccgccttc
     3721 tgcaatgcct gtgcaacgcc cgttcccgat ccgcggtctc tacgccgtaa cgccggaaag
     3781 cgtcgatcgc gcgcagctgc tggcgtttgc gaaacaggcg ctggccggcg gcgctgtcct
     3841 cctgcagtac cgcgacaaaa gcgccgattc tgccgaacgg ctggcacgcg ctgccgagct
     3901 gcgccagctt tgctcggcag ccggggtgcc gctgatcgtc aatgacgcgg tcgacctggc
     3961 gcttgccgtc ggcgccgacg gggtgcatct gggtgccgac gacggcgacc cggcagccgc
     4021 ccgcagccgt ctgggcgacg gcgcgatcgt cggtgtctcc tgctacgcga gccttgagcg
     4081 cgccgagcgc gcgctggccg ccggtgccga ctaccttgcc ttcggcgccg ccttcgcctc
     4141 gccgaccaag cccgccgcgg tgccggtatc cttgccgacc attgccgagg cgtgccggcg
     4201 cttcccggcg cgcgtcgcgg tcatcggcgg catcaccgcc gagaatgcgg tgcccctcgt
     4261 cgcggcaggc gtcagcctgc tggcggtgat taccgacctg tgtgccgcgc ccgacctcat
     4321 ggcgcgcgca cggcaatacc agtctctctt tacaggatca ggccatgacc acgcgtaacg
     4381 aaatgctgtt ccagaaagcc cagcggcaca tccccggtgg cgttaattca ccggtgcgcg
     4441 ccttcggctc ggtcggcggc acgccgcgct tcttcgcaga ggcgcgcggc gcctacgcct
     4501 gggacgccga tggcaagaag tacatcgact acgtcggctc ctgggggccg gcgatcgtcg
     4561 gccacgcgca ccccgaggtg ctgaaggcgg tgatcgccgc tgccgagaag gggctttcct
     4621 tcggcgcgcc gaccgagcag gagatcgtcc tcgccgacgc cctgtgcgac cacctgcccg
     4681 gcatggaaat gattcgcttg gtctcctcgg gcaccgaggc gacgatgagc gcaattcgcc
     4741 tcgcccgcgg tttcaccggg cgtgatggcc tgatcaagtt cgaaggctgc taccacgggc
     4801 actcggacag cctgctggtg aaggccggct cgggggcgct gaccttcggc aacccgagtt
     4861 cgggcggcgt gccggccgat cttgccaagc acacgatggt tctcgactac aacgatatcg
     4921 atcagctcga gggcgcattc gccaggcacg ccgaccagat cgcctgtgtc atcgtcgaac
     4981 cggtcgccgg caacatgaac ctgatcgcgc ccaagcccgg ctttctcaag gcgatgcgcg
     5041 agctgtgcac ccagcacggt gctgtgctga tcttcgacga ggtaatgacc ggcttccgcg
     5101 ttggccccaa gtgtgcgcag ggcctctacg gcatcgttcc cgacctgacc acgctgggca
     5161 aggtgatcgg cggtggcatg ccgcttggcg ccttcggtgg gcggcgcgac atcatggaga
     5221 agatcgcgcc gcttggaccc gtctatcagg ccgggacgct gtcgggcaat ccggtggccg
     5281 tcgccgccgg cctggcgaca ctgaagatcg tgctcgccga cggcttctac gaagcgctga
     5341 cggcaaagac caaacgtttc gccgagggca tggcgacagc aggccgggat gccgggcggg
     5401 cgttttcggc ccagtcggtc ggcggcatgt tcgggcttta cttctcggcg acgccgccga
     5461 ccagctaccg tgaaatgatg gctggcgacc gcgaggcctt cggtcgcttc ttccacgcga
     5521 tgctcgacga gggggtctat ctggcgccag cggcgttcga ggccggtttc gtctcggccg
     5581 cgcacagcga cgccgacatc gacgcgaccg tcgccgctgc gaagcgggct ttcgcgaaga
     5641 tctgagccga gcggggccgg cgcttagagc aggaagagcg ttgccagccc gaggaagatg
     5701 aaaaagccgc cggagtcggt gagcgcggtg atcagcaccg agccgccgac cgcggggtcg
     5761 gcgccgaggc ggtgccgcag catcggcacc agcacgccgg ccgaggcggc gagcaagagg
     5821 ttaagcagca tcgcggcggt cattacgacg cccagcgccg cgctcccgta gagccaccag
     5881 gcgatcaacc cgagcagtcc gccccagacg acgccgttaa acagtgcgac gccgagttcc
     5941 ttcttgagca ggcgctgcat cgccgagggt tcgacctgac cggtggcgat cgcgcggacg
     6001 atcatggtga tcgtctggtt tcccgagttg ccgccgatgc cggcgacgat gggcatcagc
     6061 gctgccagcg cgacgagttt ctcgatcgat ccctcgaagg cgccaatcac ccgcgaggcg
     6121 acgaaggccg tcactaaatt gacggccagc caagcccagc ggttctttac tgagtcccag
     6181 accggcgcga agatgtcttc ctcctctcgc aggccggcct ggttaaggat ttcggtctcg
     6241 ttctcctcga ccacgtagtc catcaccgcg gtcagcgtca gacgaccgat cacccgctcg
     6301 tcagcgtcga ccaccggcgc cgtcaccagg tcgtagcgtt cgaaggactg cgaggcatcc
     6361 tcggcttcgt cgatcggctc gaagctgatg aagtcgttgt ccatcagcga gctgacttcg
     6421 acctcggggt cggagaccag gatggcgttg atcggcagca cgccgcgcag gcgattgtcg
     6481 cggtcgacga cgaacagctt gtcggtatgg tcgggcagtt cgcgaaggcg gcgcaggtag
     6541 cgaagaacga cctcgaccgt cacgtcttcg cgcacggtca ccatctcgaa gtccatcagc
     6601 gccccgaccg aaccctcggg ataggacatc gccgcgcgca actgctcgcg ctcctcggcg
     6661 ggcagcgcct ggaagacgtc ggcgatgact tcttcgggca ggtcggggac gaggtcggcg
     6721 agttcgtcgg cgtcgagctg ctcggcgacg gcgacgattt cccgcgtgtc catcgtttcg
     6781 atcagacatt cgcggaccgc atcggagact tcgagcagga tctcgccgtc gtgctcagcc
     6841 ttgaccagat cccagacgat catccggtcg tcaagcggca gcgcttcgag gatgaaggcg
     6901 atatcggccg ggtgcatgcg gtcgagccgc gctttcagct cggcgaggtt ctgccggtga
     6961 acgaggtctt cgaccaattc gtggcgctgc atctcctggc gttcgacgag atcctcgacc
     7021 accttctgcc gcgcgagcag acgctgcacc tcgcccaggc ggtgctgcaa ctcttcgctg
     7081 tcagcgtgtt cgatttcggc gtccatatcg ctccgcggcc tgccgacggc ggtgccgaca
     7141 ggccgcggat tgtagccgct cgacccggca ggcgccacgc gggcgccggt cgtgctcagc
     7201 ggatcagggg attgccgcgt cgggcattcg ctcgacgatg atcagcgttt tgcgcgccag
     7261 tccgggggct ccgcgattgc gatcgtagta gcgcgggttg ggcagtgcgc cggcgagcca
     7321 ggcggcctgc caggcgtcga ggccggcagc ggagacgccg aaatagtgcc gcgccgccgc
     7381 ctcggcgccg aaaacaccgt taccccattc gacgacgttg aggtagatct cgaggattcg
     7441 ctgcttgtcc atcgtcgcct cgatcatcag cgtgatcagc gcctcctccg ccttgcgcca
     7501 gtaggaacgt tgtggcgtca ggaacaggtt tttcgccagt tgctgcgaga tggtcgagcc
     7561 gccggcgacg gcgcgaccct tctgctggtt cttctcgatc gcccgctgga tgccttccca
     7621 gtcgaagcct tcgtgatcaa cgaacttcgc gtcctcggca gcgacgatgg cgcgcttgag
     7681 gtggttcgat atccgtgcgt aggggaccca gcgctgcttg atctcggcct cgggtttgcg
     7741 gacgcgcagt tcggccagtc ggatgtccat gaagcgtgtc gattcgggat cgtgccgcga
     7801 ccaccagagc acatgcccga acagccagac ttgataggcg aggacgacga gcacgatgcc
     7861 gaccagcagg agcatggtgc cgcgcgccag tcggcggcgg aaccggatcg cgaagggcat
     7921 gcgggcgtca gcgccgggat gccggcgtcg ccagatcggc gcgcagcgcc gcgaataccg
     7981 gcgcggtgtc gggccgcttg ccgcgccaga gcgcgaaggc ctcggcggcc tgctcgacca
     8041 gcattcccag tccgtcggcg aagcgcagcg gcggcccatc ggggcgcgcg aggccgcgta
     8101 cgaaagcgag aaaggcagtg tcgccggcgc cgtacatcat gtcgtaggcg agcgcgccgg
     8161 cgccgaagtg gcaggcagcg accggcggca ctgcaccggc gagactggcc gaggtcgcgt
     8221 tgatcacgac atcgaagccg cgcgccggca gctcggcgaa accgattccc gcgacccgcg
     8281 agcgatcgcc ggcctgcgcc gccagtgctt gcgcgcgctc gggcgtgcgg ttggcgatca
     8341 gaatctcggc ggggttttcg gcgagtagcg gcgcgagcac gccgcgcgcg gcgccgccgg
     8401 cgccgagcag cagcacccgg cagccggtga gttgctggcc gaggttggct cgcaggtcgc
     8461 gaaccaggcc ggcgccatct gtgttgtcgc cgagcaggcc ctcggcgctg accgccagcg
     8521 tgttgacggc gccggccgct tcagcgcgcg gcgtgcgccg gtccgcgagc cgccaggcct
     8581 cttccttgaa gggcacggtg acgttggcgc cacggccgcc ggcggcaaga aagccgccga
     8641 gcgtcgcggc aaagccgtcg atcggcgcga ggatggcctc gtagtgcatg tcttcgccgc
     8701 actgcgcggc gaactgcgcg tggattgccg gcgacttcga gtgggcgatg gggttgccga
     8761 ggacggcgta gcgatcagtc acgggggcct gcgctcattg cgaccgcagc gcatcgtcgc
     8821 gggtgaagaa ccaggttcgg gtgatctcga tgatgtcggt gtcgcggcga atatccgacg
     8881 gaaacggcgc gaacggcgcg gccatttgga cgatgcggcg ggcggcgtcg tcgagcacct
     8941 tgtgtcctga cgagcggttg acctcgaccg cctcgacctg cccgtcggca cggacgatga
     9001 cggtcatgat caggttgccg tagagcttgc cgcgcgccgc cggcgggtag ttgagcgtgc
     9061 cgatgcgctc gaccttgagc cgccagtcct cgacatagcg ggcaaatcgg tactcggcgg
     9121 cacgggcgcc aacgaatgcg cgccgcgggc gcttggcgta ggcctcgagg ttgcggtcga
     9181 tctcagcctg cagccgggcc atcgccagcg cattgtcggc gaggtctcgc ccagagaccg
     9241 ttggtggcgt ttcctgcggt tgtgcgttct tggtcttgcc ggcattgacc ttcggcgcct
     9301 cgcgcttgat ttgtgccagc aggtcgcgtt gcagcttctc gagctggaag acgcgctgct
     9361 gtgccgcttc gagcttgtcg ccggcgatgt gctgggtcga tgccggcagc ggcgtccggg
     9421 cgcggcgcga ttcatcggtg ttgccgccgc cgtcaaggtt cgactgcgcg agaacctcgg
     9481 ccttctgcgg cttgcgcgtg gtcttggcgt taactaacac gacatcgagc gggcgttcgc
     9541 ggaagatccg ttcggcactg ggtaggccga aatggatgct gaggatcagc gcgtgcgcgg
     9601 caagcgaggc gccgagcgcc caggcgaagc ggcggtctgc cccgtaggcg ttgcgcatcg
     9661 acgaagcgat cgccgccacc atgttcaggc gatttcctcg acggcgtcgt cgtctgcccc
     9721 gctgtgttcg gccagcgttt cgaggtggcg ggcgtcgacg ccgagcgcca gctcgtcgat
     9781 gctggcgatg cgcagcagga tcgccgtgcc cggtgtgagc gccggcgccg aggagacgtt
     9841 gaggaccagc ggcagatggt cgaggcggaa ctgcccttcg cggcgcatcg ccgtcgcgcg
     9901 cagcggcgtg tcgccgacca gtccctcctg ggccagccag cggaggcacc agaaacgttc
     9961 gagctgacgc tggaactcgg cgtagctggc ataggcgatc tcgaaatcgc gcagtgcacc
    10021 gaacagcgct tccgagcggt tggcgaaggg tgggttgtcg tcgcgtaggc aggcgacgag
    10081 ctgccactgg ttgagcaggt cggcgtagcg gcgcagcggc gaggtacacc aggcgtactg
    10141 ggcgacgccg agtccctcgt gtggtgccgc ctgggtgctc atgcgtacct tgcccgaggt
    10201 ctgcgttcgg tagaggccgg cgacccggtg ctcgtcgagc agtccgcccc aggtcgagtt
    10261 ggcgacgatc atcagctcgg atacgagtag gtcgatcggg ctgccgcgac ggcgttcctc
    10321 gatgtggatg cggcagttcg agagatcggc gcccggcgac gagacatcgc cgtcaacgcg
    10381 gaagtggaag tcgaagatcc cctgcttggc ggccggcttg ccgcgccgcg attcgcaggc
    10441 gagcgccagg cgccagagcg tttccagctc gcgggcgaag cggaactcgc cgaggccgtt
    10501 ggtcagcgtc tgttcgttga acagcggctc gagttcgtgc aggcgcagat tggcggcgat
    10561 ggcgacgcgc tcgattcggc tttcgtggcc gaggatctcg aactgctcgg ataccgtcag
    10621 atagagcgag accgccggcg catcgcgccc ctcgatcagc gtacagctgc cgacccagtc
    10681 gtcgggcagc atcgtgatct tgtgccccgg catgtagacg gtcgacaggc gcgcccgcgc
    10741 ctgggcgccg gccggcgaat cgggcgtgaa ggcgagcgcc ggcgcggcaa tgtggatgcc
    10801 gatgcgccag ccgatgccgg gcagggcctg caccgaaaag gcgtcgtcga tctcggtcgt
    10861 ctgtacgtcg tcgatcgaga acgccgccgc ttcggcgcgc ggtagctcgg cgccgaaact
    10921 gccgctgggc ggcgccggca cttcgctcgc gtcaggaaag ttggcgaaga gaaagccgcc
    10981 gagatggaaa tcgtagggcg agtgcagcgc gccgcagcgc agcagcagct gcgccggcgt
    11041 ggcctgcgcc tgctcgcagg cggcttcgag cgccttggtc tcgagccggt tgcgatccgg
    11101 gcggtagagg atcgccggca ttgcctgggc aatctcggcc ggcaaatcgc cgccggcaag
    11161 ctgggccgcg aggcgctcga ccgtttccgc ctgctgccgc ttcttctcct gcccggccag
    11221 cgctgcggcg aggatgtcgg gcggtgcctt gcggaagcgg cccttgccct tgcgatgaaa
    11281 gtggatcggc gcggcgtgta gcgccagcag cacggcagcg gcctcgaccg cgctcggccg
    11341 gtggccgtag tagtcgttgg cgaaatcggt gaaggcgaac tcgccgtcgc cgcagcattc
    11401 ccagagaaag gcggcgtcga tgccggcggc aagcggctcg gcggccggca gcagttcgcc
    11461 cggtgccgga tcgccgaact caaggagcac ctgcgcgcgc ttgatcttgg cgcgtttgcc
    11521 ggtggccgtt tcgacctgca gcgaggtcgt gttgtcggcc agaatcgtgc cgccgcggaa
    11581 actgccgtct tcttcaaaaa gcaggtgcat ggccgattat atctggggct gcgcggcgtt
    11641 tcgggcggcg tcggcgtagt cgacgatcgt cggcaggaac tcggtgaagc gcgtgaaact
    11701 gtggtcgccg ccggccaaaa cgacctgccg ggcgccggca agcgcggtgg ttgtgacctg
    11761 cgcatcgagc acctcgtcac cggtctcgac cagcagcagg tagcggttcg gcgtgagatg
    11821 ccgcggtgcc atgtcgagca gcgcctgctg gtgtgccgcg gtgaaggtga aggcctcgcc
    11881 gctgtggaag ttgcggtgtt cgccgatcag cggcacggtt tcgaggtgcg tcggcagcgc
    11941 cggattgacc agtaccgctt gcagaccgtg ccgttcggcg aggcaggtgc tgtagaagcc
    12001 gccgagcgag ctgccgatca gcgttgccgg cgacggcaag tcggccagcg ttgcctcgat
    12061 cgcagcgatc gcctgaaccg ggtccggcgg cagcgccggg cagcggaatt cgttggcgcg
    12121 gccgaggcgc gcgaggtagt cagcgagctg ccgggccttg gccgatgctg gcgacgagca
    12181 gaaaccgtgc agatagacga tcaagtcgtg ctccagtcga cccagccgag ctgccagctc
    12241 gccagcacca gcaggccgaa ggcgatccgg taccaggcga agactgcgaa ggaatggtgc
    12301 gcgacgtagc ggatgaatcc cttgaccgcc cacatcgccg agacgaagga ggcgacgaaa
    12361 ccgacggcga agatcggcag gtcgtcggcc gagagcagcg cccagttctt gtagaggtcg
    12421 taggcggtgg cggcgaacat ggtcgggatc gccagaaaga aggagaactc ggcggcggtc
    12481 ttgcgcgaca ggccgaagac gaggccgccc atgatcgtcg cacccgagcg cgaggtgccg
    12541 ggaatcattg ccagcgcctg cgcgaagccg accttgagcg catcgcgcca gcccatgtcg
    12601 tcgacctcgt ggatgcgtgc gctatcggca cggcgctcga tcaacaggat caggaatccg
    12661 ccgacgacca gagccgtcgc caccgtgatc ggattgaaca gcagtgcctt gatcgccccg
    12721 tggaacagca ggccaagcac tgcggcaggc agaaagccga cgaacagcag gaggacgaag
    12781 cggttcgcgg ccggttcgcg gccgaagccc attgccacct tggtcagccg ttcgcggtag
    12841 tcccagcaga cggcgaggat ggcggcaagc tggatgacga tcttgaagac cttgctggtc
    12901 tcgtcgttgt aaccgaccaa gtcgccgacg acgatcaggt ggcccgttga ggacacgggc
    12961 aggaactcgg tcacgccttc gacgatccca agcagtaggg cggccagcag cgcgtccatg
    13021 ggtgtgctcg aaaaggttag aatgctagca cgctgtcagt agccaggcgc cggccgggat
    13081 tcgcccgatt ccctgactgg gtaaggcttg tcgcaaggag tgccgcatga ccaagaaaat
    13141 ctgcaagtgc ctgctgatcg tcggcttcgc cgtcggcgtt tcggctgttc acgcgcaggg
    13201 tcttcgcgcg ccggccgcag gcctggctag tgacgggctg cttgatcgct actgggagag
    13261 cggcgggctg aagagcttca tgcagacctt gccgaaaacc gaacgcgagt ccgggccgct
    13321 gctcggtggc tggtcctacg cggttggcga ggattcagga ttcagtgtcg gactcggttg
    13381 gcagccggga gagaccaccg aggacatcgg ctatctcgga gctgccggcg cgagttggcg
    13441 gcagcgcttc tacggcttct gggatccggt gattacggct ggcctgtcga tcatgcgtca
    13501 gccgcatcag gagagcggtc tggtcggcca gttgtacgga gcgcatgtct cgctgggcct
    13561 taacccgagc agcgactggg gtatctcgct cgggctcggt tatcgcgatg cgatggatgc
    13621 caatccgctg gccacggctg ccgagcgtgc cgatcagcgc gccgtctacg gcgatctcgc
    13681 cgcggtttat cgcctgcagc gcaatctcga gttgcagggg cagatcaccg ggcttgacct
    13741 ccgctcgcgc agtcttgccg caaaccccga cagcacgcgc gaagtcggca tccgcatgcg
    13801 ttacttcttc gactgagctg aaggcggcag ggctcgcggc ctgcgccgca actcgcccag
    13861 gcgaccggtc gccagcagtt tctccaggcg cgccagattg ctgctggccg cctccaggta
    13921 gggattccgt ccaccgaaat gcacgtcgag gcaggtgtgg cagcgggcga aacgctgatc
    13981 gagtcgttgc gggccgaagc tggcaaagaa tcccggcgtc tgccgcaaca ggtcctcgcc
    14041 cgagagatat agaaccgtcg ttgagccatc agcctcctgc cgacggtcca tgccggcgcg
    14101 gacgaactcg gtgtagagaa agtcccggca tttctcgagg tagtagcggt cagcgatttg
    14161 cgcgaggaag tcggcggtac cgatgatgtt ggccatcgct gcgcgtcgtt gatcagcgac
    14221 ggccgcggat gcgagcgcgt cggcggcgat gttggttgcc tggatcagct cggcgcaggc
    14281 ggcgagttcg ggccacgcgg ccgccaacgg tgaggcacga acccaggcga cgctgcgcgc
    14341 ctcgtgctca gccatgaagc gcgcgccatc ctggccatct tcctggtgac gactgcgcag
    14401 aaaccccgtg tcatgtagca gcgcgagtac ggtcgtggtc gccgccagtc gggcgccgag
    14461 gcgctgttcg gccgatttcg cggcaatgtc gaagccatcg gccaagcgcg ttgcgcaaag
    14521 tgccgtgtcg agcgcatgac gcaggtcgtg atagggcgtg tcgcaggcga gccagtcagg
    14581 cgattcgccg aaatacgcgc gacgcgcctc ctcgaaggcg cggaacagga actgcgaatc
    14641 gacggcgtct ttgccgtaac ggtgctgctg gatggcgata caggcgcgcg cgacggcgct
    14701 cgggtcgtcg gtgcgaaccg tgccggtcac gtcgagcgct cgggtgttcg acaacatcga
    14761 agtggcgccg ccgcttcagc ggcgcaggcc ttcctcgtcg agggtgcgca ggacggcagg
    14821 gcgggctgcg atctgggcca ggtaggcaga caacgcctgc cagcgatcaa gcggtatctt
    14881 caaaaagctg gtccagttga gcacggtgaa aaggtaggcg tcggcgaccg tgaatcgatc
    14941 acccatgagg aagggccgct taaccagttg cggcgtcagc cagtcgaaac gccgttcgag
    15001 cgcggtgctt gctgcctggc ggatttcgtc gttctgcgag tgccagagcg ggctgaatgt
    15061 cttgtgcagc tcggtgccga tgaagctcag ccactcctgc agacggtagc gcggcaaggt
    15121 gccggccgcc ggcgcaagcc ccgagtcggg tgcgcggtcg gcgatgtact ggacgatggc
    15181 gggcccttcg gtgaggaagg cgccgtcgtc gagccgcagc acggggacgt agcccttgct
    15241 gttgatcgtc gagaaatcaa tgcccgcctc ggtcttccgc gtcttcaggt cgacgcgctc
    15301 aagcgtgtag ggcaggcccg tctccgccag cacgatgtgc ggcgagagcg agcaggcccc
    15361 gggcgagtag tagagcttca tggggtttgc tcctcggatg cgatggactc agtctggatg
    15421 ttagccacgc cggcgggctt ccgctcagcg gaatgaagcg gagcgccgca atctgcgcgg
    15481 gagctgctca cccgcggcgg gggctcagtc gcggaagagc cgcccgcgtg cggacaggtc
    15541 ggcgcgccag tactgcggcg gcgcatcgac ctgggcgccg agttcggcgg cggcatgcca
    15601 aggccagcgc ggatcgtaga ggatgccgcg ggcgagggcg acgagatcgg cgtcgccgct
    15661 gccaagtatt tcctcggcct gctgcgcccg cgtgatttcg ccgacggcga tcgtcgtcag
    15721 tcgggtttca gcgcggatcg ttcgtgcgaa cggcacctga tagccgggtc cggcgacgat
    15781 ctgctgggcc ggcgagaggc cgccgctgga gacatggatg tagtcggcgc cgcgcgcctt
    15841 gagttcgtgg ccgagcgcgg cggtctgcgc gacatcccac ccgccttcga cccaatcggt
    15901 cgccgagacg cgcatgccca gcggtttgcg ctcgggccag accgcccgta ccgcgtcgaa
    15961 gatctccagt gggaagcgca gacgattggc cagcgaaccg ccgtagtcgt cggtccgccg
    16021 gttcgctagc ggcgagagga actgatgcag caggtagccg tgcgcgccgt gcagttcgat
    16081 gccgtcgaag ccgagtcggt cggcacgttg cgcggcgtcg acaaaggatt gcctgatccg
    16141 ggccagcccc gccgcgtcga gttcgagcgg cacgcgatcg gcgtcccgaa aggccagcgg
    16201 tgaaggggcg agcgtgttcc agccgccggc gtcaagcgcc agctgccggc cgccgagcca
    16261 aggtgctgcg caggacgcct tgcggccggc gtgggcgagc tggatcgcca gcggcatcgc
    16321 cgagtggcgg cggaccccgg ccagcacgcg ggcaaaggcc gcctcgtgtg cgtcgctcca
    16381 caggccgggg cagcgatcgg agatgcgacc ttcgggcgtg accgcggttg cttcgacgat
    16441 cagcaggccg gcgccgctgt gcgcgaggct gcccaggtgg atcaggtgcc agtcgtcgat
    16501 caggccatcg acggccgagt attggcacat cggcgcgatg acgatgcggt tggccagcgt
    16561 cagcccgcgc agcgcgatcg gcgaaaagag tgcggatggc atctgtgcgg acatcgggcg
    16621 ttcagacttt cttgtacagc tcggcgccgg tcttcacgaa ctcgacggcc ttggtttcca
    16681 tgcccttagt cagcgcctcg gtctcggcaa tgccctgcgc cgcggcgaac tctcgtactt
    16741 cctgggtgat cttcatcgag cagaagtgcg ggccgcacat cgagcagaaa tgcgcgacct
    16801 tggccgactc cttgggcagc gtttcgtcgt ggaagctctt cgccttgtcg gggtcgaggc
    16861 cgaggttgaa ctggtcgtcc cagcggaatt catagcgcgc ctttgagagt gcgttgtcgc
    16921 gaatctgcgc gcctgggtgg cccttggcga ggtcggcggc gtgcgccgcg agcttgtagg
    16981 tgatgatgcc ttccttgacg tcgtccttgt cgggcaggcc taggtgctct ttcggcgtca
    17041 cgtagcagag catcgccgtg ccgtaccagc cgatcatcgc cgcgccgata cccgaggtga
    17101 tgtggtcgta gccgggggcg atatcggtgg tcagcgggcc gagcgtatag aacggcgcct
    17161 ccttgcagtg cttcaactgc aggtccatgt tctccttgat caggtgcatc ggcacgtgcc
    17221 ccggaccttc gatgatcgtc tgcacgtcgt gcttccaggc gatgtcggtc agttcgccga
    17281 gggtttcgag ttcaccgagc tgcgcttcgt cgttggcgtc gtagatcgag ccgggacgca
    17341 ggccgtcgcc gagcgagaag gcgacgtcgt aggccttcat gatttcgcag atgtcctcga
    17401 agtgcgtgta gaggaagctt tccttgtggt gggccaggca ccacttggcc atgatcgatc
    17461 cgccgcggct gacgatgccg gtcatgcggt tggcggtcat cggcacgtag cgcaagagca
    17521 cgccggcgtg aatggtgaag tagtcgacgc cctgctcggc ctgctcgatc agcgtgtcgc
    17581 ggaagatgtc ccaggtgagg tcctcggccc tgccgtttac cttttcgagc gcctggtaga
    17641 tcggcacggt gccgatcggc accggcgaat tgcggatgat ccactcgcgc gtttcatgaa
    17701 tgttcttgcc ggtggacagg tccatcaccg tgtcgccgcc ccagcggatc gacctggtca
    17761 ttttctcgac ttcttcctgg atgctcgagc cgagcgccga gttgccgatg ttggcgttga
    17821 tcttcaccag gaagttgcgg ccgatgatca tcggctccga ttccgggtgg ttgatgttgt
    17881 tggggatgat cgcgcggccg cgcgccacct cgctgcgcac gaactccggg gtgatctcgc
    17941 cggggatcga cgccccgaag tcctggccct tgtgctgacg cgtcagcagc ttggccatct
    18001 tctcgccctg cgcaccggtg gcgcgcagcg actcaatgta ggcggcgcgg ttgttgttct
    18061 cgcggatcgc gacgaactcc atctccgggg tgatgatgcc cttgcgtgcg taatgcatct
    18121 gactgacgtt catgcctgct ttggcgcgca gtggtttgcg gtgcaggccg ggaaagcgca
    18181 gttcgtcgag ggccttgtcg gccgcgcgct cgcggccgaa ttgcgacgac aggtcgggca
    18241 gcgactcgac gtcgccgcgc tcggcgatcc attgcgcgcg cattgccgga aggcctttgc
    18301 ggatgtcgat cttcgccgcc gggtcggaat aggggcccga gcagtcgtag acgaagaccg
    18361 gcgggttggc ctcgccacca aagcccgtcg gcgtcgtcgc ctgcgagatt tcgcgcatcg
    18421 gcacccgcag gtcggggcgc gatccttcga tgtatacctt gcgcgaattc ggcagcggtt
    18481 cgacggccgc cgagtcgaca tgggcggtcg cggcgatgaa ttgatccttg gcgttcatgg
    18541 ggtgactccg gtcgttgggg cggcgcggct ttgcagaagc acgcgcttat cgccaaaggg
    18601 cgtggaagtg gtggaccggg ccgtggccgg tcggcgtcga tgcgactgcg agatcgcccg
    18661 aacgttcaat cgcgcgcgcc agccaacggt gcgcgtcaag cacggcgctc ggcacctctg
    18721 gtcgttgcgg cagcagcgcg gcgatggcgg ccgagtaggt gcagccggtg ccgtgcgtgt
    18781 tgttggtttc gacccgacgg ctttcgagtt cgatcatgtt gtcgccatcg aacagcaggt
    18841 cgagcagatc gccgccgggc aggtggccgc ccttgagcag cacccagcgg cgcccgctgt
    18901 gcggcaggat ttcgcgcaac ttctcggcgg cgcggcgcat ttccttcggc gattcgggcg
    18961 tgctcagctt gagcagcaca gcggcctcgg gcaggttggg ggtgatcatc gtcgccagcg
    19021 ggaacagcgc ttcgcgcagc atcgcaatcg cgtcgtcggc gagcagcctg tcgccgctct
    19081 tggcgaccat caccggatcg agcaccagat agggcagggg tcgcccgagc agcgcgtcgg
    19141 cgactgtgcg caccacccct gcggtgccga gcatgccgat cttcgcgctg gcgattgcca
    19201 cgtcagcgaa cagggtgtcg atctgcaggc gaatgaaatc ggtgggtggc agatgcacgc
    19261 cggtgacgga ccgcgtgttc tgcgcggtca gcgccgcgac gacgccgcag ccgtatgcgc
    19321 cgagtgccga gaaggtcttg aggtcggcaa agatgccggc accacccgag gggtcaacac
    19381 cggcgatcga gacgacgttg ggggtgcgac tgggtgatgc ggacgacgtg gcagatgcga
    19441 tcaacgtttt cctccgccgg gatcaaccgg atcaggttcc aagggtctct ctcagccagc
    19501 ccggcgcgcg ccgtacttgg cacccctaac gcgaggaggc attctatcag gcggccggta
    19561 tcgtctcggc tgcgcaagcc ggtgcgacgc cggctgggca tgacggcctc
//
LOCUS       DATDAA010000346         3365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_340176,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000346 DATDAA010000000
VERSION     DATDAA010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340176"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1737)
                     /locus_tag="VIS73_10460"
     CDS             complement(<1..1737)
                     /locus_tag="VIS73_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEY5763615.1"
                     /translation="MDLQTITYLVVGASFALYIGIAMWARAGSTSDFYVAGGGVPPVM
                     NGMATAADWMSAASFISMAGLISNLGYGGGLFLMGWTGGYVMLACLLAPYLRKFGKFT
                     VPQFIGDRYYSQTASTVAVICLLVASITYIIGQMTGVGVAFSRFLGVSSSTGIYIGMG
                     IVFMYAVFGGMKGITYTQVAQYLVLISAYTVPAIFISLQLTGNPIPQLGLGSNLVGQD
                     VSLLAKLDQIVTELGFGQYTTSVPANSTLNMFVYTFSLMIGTAGLPHVIMRFFTVPKV
                     RDARLSAGWALVFIAILYTTAPAVAAMAKTNLIKTVTPGVVAGTADTFAADSQIMYEE
                     RPDWMKRWEVTGLLKFDDKNGDGRIQYYNDKNKNPEFLAKAEGAGWKGNELTVNADII
                     VLANPEIALLPNWVIALVAAGGLAAALSTAAGLLLAISSAISHDLIKNVFMPDISEKG
                     ELLAGKISMAGAIVVAGYLGLNPPGFAAGTVALAFGIAASSLFPAIMMGIFSKKMNKE
                     GAIAGMLVGLGVTLFYVFAHKGIFFIKGTGYVDLIGGPNSFFGITPEAFGGIGALINF
                     MVAFAVDKVTKEP"
     gene            complement(1749..2003)
                     /locus_tag="VIS73_10465"
     CDS             complement(1749..2003)
                     /locus_tag="VIS73_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HEY5763616.1"
                     /translation="MAEDVKGYWPAVLGLITKVFVIWFLVSYGAGILFAPMLNSISLG
                     GYPLGFWFAQQGSMYVFVGLIFWYAKQMDKIDQRFDVHED"
     gene            complement(2166..2534)
                     /locus_tag="VIS73_10470"
     CDS             complement(2166..2534)
                     /locus_tag="VIS73_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY5763617.1"
                     /translation="MDIITLEALRAETLLGVYPRERVLAQTVEIDLAIGFSSASAGAS
                     DDLADTIDYAEVVTRIRGDLAASRFHLLEALAEHVASLLLAEFGASWVRVSVTKLGVI
                     PGVRRVGLTIERSLGTGLPE"
     gene            2604..3215
                     /gene="plsY"
                     /locus_tag="VIS73_10475"
     CDS             2604..3215
                     /gene="plsY"
                     /locus_tag="VIS73_10475"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY5763618.1"
                     /translation="MTASPLLLPLMLLAAYLLGSIPFAVVSSRLFGLADPRSYGSGNP
                     GATNVLRSGSKAAAALTLIGDSAKGAIAVALGAAAGLADAGLIAVGLAAFLGHLFPLF
                     LGFKGGKGVATAAGVLLAIDYRLGLGVLAVWVGVAVLSRYSSLAALVAAASAPLLAYL
                     MGGTSAGGIALLGLMSVALIAKHHANIKRLLAGEEGRIGRKKA"
BASE COUNT          668 a         1063 c         1049 g          585 t
ORIGIN      
        1 cggctccttg gtcaccttgt cgaccgcaaa ggcaaccatg aagttgatca gcgcgccgat
       61 accgccgaag gcttccggcg tgatgccgaa gaagctgttg ggtccaccaa tcagatcgac
      121 gtaaccggtg cccttgatga agaagatgcc cttgtgcgcg aacacgtaga aaagcgtgac
      181 gccgaggcca accaacatgc ccgcaatcgc accttccttg ttcatcttct tggagaagat
      241 gcccatcatg atggccggga acagcgacga ggccgcgata ccgaaggcca gcgccacagt
      301 acccgccgcg aatcctggtg gattcaggcc aaggtaaccg gcaaccacaa tcgctccagc
      361 catcgagatc ttgcccgcga gcagttcgcc cttctcggag atgtccggca tgaacacgtt
      421 cttgatcagg tcatgcgaga tcgccgagga gatcgccagc aacagacccg ccgcggtgga
      481 aagcgcagca gccagaccac cggcagcaac cagcgcgatg acccagttcg gcaacaaggc
      541 gatttccggg ttggccaaca cgatgatgtc ggcgtttacc gtcagctcgt tgcccttcca
      601 gccagccccc tcggccttcg ccaagaattc cgggttcttg ttcttgtcgt tgtagtactg
      661 gatgcggccg tcgccgttct tgtcatcgaa cttgagcagg ccggtgacct cccagcgctt
      721 catccagtcc ggacgctctt cgtacatgat ctgcgaatcg gcggcgaacg tatcagcggt
      781 accggcaacg acgcccggcg taaccgtctt gatcaggttc gtcttggcca tcgcggcaac
      841 ggcgggagcc gtcgtgtaca ggatggcgat gaagaccagc gcccaacccg ccgacagacg
      901 agcgtcacga accttcggca ccgtgaagaa acgcatgatg acgtgcggca ggccagccgt
      961 accgatcatc agcgagaagg tgtagacgaa catgttgagc gtcgagttcg ccggcaccga
     1021 ggtcgtgtac tggccaaaac caagttcagt aacgatttgg tcgagcttgg cgagcagcga
     1081 gacgtcctgg ccgacgaggt tcgaaccgag gcccagctgc gggatcgggt tgccggtcag
     1141 ctgcagcgaa atgaagatcg ccggaaccgt gtaggccgag atcagtacca ggtactgggc
     1201 gacctgggtg taggtaatac ctttcatgcc gccgaacacg gcgtacatga agacgatgcc
     1261 cataccgatg tagataccgg tcgaggacga cacaccgagg aagcgcgaga aggccacgcc
     1321 gacaccggtc atctggccga tgatgtaggt gatcgaagcc accagcaggc agatcaccgc
     1381 aacggtcgac gccgtctgcg agtagtaacg gtcaccgatg aactggggaa ccgtgaactt
     1441 gccgaacttg cggaggtagg gcgcgagcag gcaggccagc atcacgtagc cgccggtcca
     1501 gcccatcagg aacaggccgc cgccgtagcc gaggttggag atcaggccgg ccatcgaaat
     1561 gaacgaggct gccgacatcc agtcagccgc cgtcgccatg ccgttcatga ccgggggaac
     1621 gccgccgccg gcgacgtaga aatctgaagt cgatccggca cgtgcccaca tggcgatgcc
     1681 gatatacagc gcgaaggagg cgccgacgac cagatacgtt atggtttgaa gatccatgta
     1741 tccgctcctt agtcttcgtg aacgtcgaaa cgttggtcaa ttttgtccat ctgcttcgcg
     1801 taccagaaga tcaggccgac gaagacatac atcgaacctt gttgcgcgaa ccagaacccg
     1861 agcggatagc cgcccagcga gatgctgttc agcatcggtg cgaacaggat gcccgccccg
     1921 taggacacca ggaaccagat cacgaaaacc ttcgtgatca gcccaagcac ggcgggccag
     1981 tagcccttca cgtcttcagc cattgttatt gcctcctcga agtttgtatt gctgcgtagg
     2041 tggaaccgac ggcgtagccc caccaggact ccaccggtgc gcggagtttt aacgacccgc
     2101 cttactaaag acttacaggg cgtaatagat ttacgcggag taaaccgcgc tggcagcgcg
     2161 tttcctcatt ccggaaggcc cgtgccgagg ctgcgctcga tggtcagacc aactcggcgg
     2221 acgccaggaa tgacaccgag cttggtgacc gagacgcgga cccaggaggc accgaattcg
     2281 gcgagcagca ggctcgccac atgctcggcc aacgcctcca gcaggtggaa gcgactggcg
     2341 gctaggtcgc cgcggatgcg ggtcaccacc tcggcgtaat cgatggtgtc ggccaagtcg
     2401 tcgctggcgc ccgcactggc gctcgagaat ccgatcgcca ggtcgatttc taccgtttgt
     2461 gccagaaccc gttcgcgggg gtaaacgccg agcagggttt cggcgcgcag ggcttccagc
     2521 gtgatgatgt ccatggttct cgcgcgcgcc gttcgggcgc cggcagatac aataagggcc
     2581 cgaattctaa gcctgcagat gcgatgaccg cgagcccgct cctcctcccg ctgatgctgc
     2641 ttgccgcata cctgctcggt tcgattccgt tcgcggttgt gtcgagtcgg ctgttcggcc
     2701 ttgccgaccc gcgcagctac ggctcgggca atcctggcgc aaccaacgtc ttgcgcagcg
     2761 gcagcaaggc ggcggcggcg ctgaccttga tcggcgacag cgcgaagggc gcgatcgctg
     2821 tcgccctcgg cgcagcggcc ggcctcgccg acgccggcct gatcgcggtc gggctggcgg
     2881 cctttcttgg ccacctcttc ccgctctttc tcggttttaa gggtggcaag ggtgttgcga
     2941 cggcggccgg cgtattgctg gcgatcgact accgcctggg cctgggcgtg ctggccgtct
     3001 gggtcggcgt cgccgtatta agccgctatt cctctctggc agcgctggtc gccgcggcct
     3061 cggccccgtt gctggcctac ttgatgggcg gcacgtcggc gggcggcatc gcgctgctcg
     3121 gactgatgtc ggtggcgctg atcgccaagc accacgccaa catcaaacgg ctgctggccg
     3181 gcgaggaagg ccggatcggc cgcaaaaagg cctgaagccc gcgttcgatt gcactaagcg
     3241 ccgagcgtca gctccgccag cggccaccgc ggtcgcacgc cgaaagcccc cggatccttg
     3301 gccggcgccc ccgccgccag ccgcaaagcg cccgccagcg caatcatggc gccgttgtcg
     3361 gtgca
//
LOCUS       DATDAA010000347         4981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_235353,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000347 DATDAA010000000
VERSION     DATDAA010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235353"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VIS73_10480"
     CDS             <1..956
                     /locus_tag="VIS73_10480"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5763619.1"
                     /translation="SFFAMMQRRWRGDFEPKAEPAGGYEAFAAEWSTPVDRELIGRRH
                     GEPVITWLGHATLLLQVGGLNILIDPQFSSHAGPWGVFGAKRRVPVPIMPADLPPIDF
                     VLISHNHYDHLDAPSIRRLDAIPGNRTRYLVPLGVKAWFDDQNIGPVEEMDWWNFRQY
                     GEVAIHFTPAQHWSKRTPWDTNRSLWGGFALFYWPGGIGRPSVVPWRFLYTGDTGYSP
                     DFAEIRKRLGRFDLLAAPVGAYEPRDFMAPHHINPDEALQIMDDLGARNLLGVHWGTF
                     ELTQEAFDRPPQDLDAARQARDIAASRIWLFKHGESRRVPR"
     gene            complement(963..1541)
                     /locus_tag="VIS73_10485"
     CDS             complement(963..1541)
                     /locus_tag="VIS73_10485"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY5763620.1"
                     /translation="MAVVAPCRPRRRTGILAALALTATLLAAGEPLDAHAASGDASFY
                     ALTMPLTRPDGSAAQMADWRGRQAIIAMDHTQSLVVCSDTIRRLRAVQTAADRLGKGF
                     DYIVISLDPQADTPAQWARYKAFFDVDRPGWHFLNAGVDDTQALIERLGIRISYDQGY
                     RFHAVKLFRVDASGHLVRTLEGYDRNTEAFLQ"
     gene            complement(1554..2852)
                     /locus_tag="VIS73_10490"
     CDS             complement(1554..2852)
                     /locus_tag="VIS73_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5763621.1"
                     /translation="MLLEPLELRAGSANEALAAESLAALMRDTDTLGIVGVVILRLMP
                     DVALMRLRPEQLPDFHAAVYDLLRRNLREPDRLLRTDRFEWTVLLPALPSPAALTLAM
                     LRLTRAFETLQPELLRFSPKIDARYGGALFPDVSGDAIHLLQSARIACVEGTTTESPY
                     ALYEAHFEDKSTRQQHLSMEVAAALNASSFELHLQPQIHLGTGLCIGAEALLRWRRSV
                     NDWVAPPLVIAMIERAGLRPAFNRWLFNHTMQIAQDLSTAGIEAPISINLSAGDLQDN
                     DLAMLLEQAMQTFNITPQRVRLEITETALVNQTENAQSLVAQLGAIGVALSIDDFGTG
                     YSGMTHLRLLPVREIKIDQMFVLSIAESEIDRSIVQAILDLGRRLEIAVIAEGVETAD
                     AARVLHGMGCEAAQGYLYGRPMPVSDFIGWHRARTPAGLD"
     gene            complement(2842..4161)
                     /locus_tag="VIS73_10495"
     CDS             complement(2842..4161)
                     /locus_tag="VIS73_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY5763622.1"
                     /translation="MKKYIVPVADLVPGMFVTDLDRPWLGTPFLLQGFHVDGADQIEE
                     LRRLCKVVTVDPARSHCRRVDFTAYERDEQPAKPHDFAEEPEVAIENDDFYSAAYLVR
                     NRKLPKGRGTPPVINPSDGQSQLVAEMIYSASIVDDVQSVMNAIFASIESGVKPDFVE
                     LASLTMEMAQSVARNTDALLWLARLKETDSYSYDHAIDVSVHMMVFASFLGYPQHSVE
                     SLGLAGMLQDIGKSEVDPILLQKTGALDDDERSQIRAHVASSLDMLRKTAVFSPSLFD
                     IVANHHERHDGSGYPRGIAGEVLSLHAGMAGMLDTYCAMLRARPYSDGLSNQHVLEEL
                     NRMRGKAFRETLVDQFVQCIGLYPIGTLVDLNSGEVAVVIQQNQVRRLQPKVMVLLGP
                     DKSVERHPRTLDLLMQPKTPTGEAYRIVRALAGNAYGIDPRDYYLVA"
     gene            4351..>4981
                     /locus_tag="VIS73_10500"
     CDS             4351..>4981
                     /locus_tag="VIS73_10500"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HEY5763623.1"
                     /translation="MKYRDLREFLVGLEKRGELRRVGVAVDPHLEMTEICDRTLRVGG
                     PAVLFEQPTGHSMPVLGNLFGTPGRVALGMGEEPEGWQTSLREIGKLLAYLKEPEPPK
                     GLKDAWEKLPVLKKVLDMAPSVKSSAACQEIVWEGSEVDLSRLPIQTCWPGDAAPLIT
                     WGLTVTRGPHKKRQNLGIYRQQVIARNKVIMRWLAHRGGALDFRDHCVAN"
BASE COUNT          836 a         1545 c         1710 g          890 t
ORIGIN      
        1 gatcgttttt cgcgatgatg caaaggcgct ggcgcggcga ctttgagccg aaagcggagc
       61 cggcgggcgg ctacgaggcc ttcgccgccg agtggtcgac gccggtcgat cgcgaactga
      121 tcgggcgtcg ccacggcgag ccggtgatca cctggctcgg ccacgcgacc ctgttgctcc
      181 aggtcggtgg cctgaacatc ctgatcgacc cgcagttctc cagccacgcc gggccctggg
      241 gtgtgttcgg cgcgaagcgc cgggtgccgg tgccgatcat gccggccgat ctgccgccga
      301 tcgacttcgt gctgatctcg cacaatcact acgaccatct cgatgcgccg agcatccgcc
      361 ggctcgacgc gattccgggc aaccggacgc gctatctggt gccgcttggc gtcaaggcct
      421 ggttcgacga tcagaacatc ggcccggtcg aagagatgga ctggtggaac ttccgccagt
      481 acggcgaggt ggcgatccat ttcacgccgg cgcagcactg gagcaagcgc acgccatggg
      541 ataccaaccg cagcctgtgg ggtggcttcg cgctgttcta ctggcccggc ggcatcggcc
      601 ggccgagcgt cgttccctgg cgcttcctgt acaccggcga caccgggtac tcgccggact
      661 tcgccgagat ccgcaagcgg ctcggccgct tcgacttgct cgccgcgccg gtcggcgcct
      721 acgagccgcg cgacttcatg gcgccgcacc acatcaatcc cgatgaggcg ctgcagatca
      781 tggacgacct tggcgcgcgc aacttgctcg gcgtgcactg gggcactttc gaacttacgc
      841 aggaagcctt cgaccggccg ccgcaggatc tcgacgccgc gcggcaggca cgcgatattg
      901 ctgcctcgcg catctggctg ttcaagcacg gcgagtcacg tcgggtgccg cgctagacag
      961 ggctactgca ggaaagcctc ggtgttgcgg tcgtagccct cgagcgtgcg gaccaggtgc
     1021 ccgctggcgt cgacgcgaaa cagcttcact gcgtggaagc gatagccctg gtcgtagctg
     1081 atgcgaatgc cgaggcgctc gatcagcgcc tgcgtgtcgt cgacaccggc gttgaggaag
     1141 tgccagcccg gccgatcgac atcgaagaag gccttgtagc gggcccattg cgccggcgtg
     1201 tcggcctgcg gatcgagact gatcacgatg tagtcgaagc ccttgcccag acgatcggcc
     1261 gcggtctgca cggcgcgtag gcggcggatc gtatcggagc agacgaccag actctgcgtg
     1321 tggtccatcg cgatgattgc ctgtcgaccg cgccagtcgg ccatttgcgc ggcgctgccg
     1381 tcgggccgcg tcagcggcat ggtcagcgcg tagaagctgg cgtcgccgct tgccgcgtgc
     1441 gcgtccaagg gttcgcctgc ggcgagcagc gtggcggtca gtgcgagcgc cgcgagaatt
     1501 cctgtccggc gccgcggccg gcagggggca acaacagcca cggcccgcat gcctcagtcg
     1561 agaccggccg gcgtcctcgc ccggtgccag ccgatgaaat cggataccgg catcggtctg
     1621 ccgtagagat acccctgtgc cgcctcgcaa cccatgccgt gcaacacacg cgcagcgtcg
     1681 gcggtttcaa cgccttcggc gatgaccgca atttccagtc ggcggccgag gtcgaggatc
     1741 gcttggacga tcgagcgatc gatttccgac tcggcgatgc tgagcacgaa catctggtca
     1801 atcttgattt cgcgcacggg cagcagccgg aggtgcgtca tgcccgagta gccggtgccg
     1861 aagtcatcga tcgacagcgc gacgccgatt gcaccgagtt gtgcgactag tgactgggcg
     1921 ttctcggtct ggttgaccag cgcggtttcg gtgatttcca gccgaacgcg ctggggcgtg
     1981 atgttgaagg tttgcatcgc ctgctcgagc agcatcgcga ggtcgttgtc ctgtaggtcg
     2041 cctgccgaca ggttgatcga gatcggggcc tcgatgccgg ccgtggagag gtcctgcgcg
     2101 atctgcatcg tgtggttgaa cagccaacga ttgaaagccg ggcgcagccc ggcccgttcg
     2161 atcattgcga tcaccagcgg tggggcgacc cagtcgttga cgcttctccg ccagcgcagc
     2221 agtgcctcgg caccgatgca cagcccggtg ccgaggtgga tctgtggctg caggtgcagt
     2281 tcgaagctgc tggcgtttaa ggccgctgcg acttccatcg acagatgttg ctggcgcgtc
     2341 gacttgtcct cgaagtgcgc ttcgtacagc gcgtagggcg actcggtcgt cgtgccttcg
     2401 acgcaggcga ttcgcgccga ttgcagcagg tggatcgcgt cgccgctcac atccgggaac
     2461 agagcgccgc cgtagcgcgc gtcgattttg ggcgaaaagc gtagcaactc aggctgcagt
     2521 gtttcgaaag cgcgggtcag ccgcagcatt gccagcgtta gcgccgcagg gctaggcaac
     2581 gccggcagca ggaccgtcca ctcgaagcgg tcggtgcgca gcaggcggtc gggttcgcgc
     2641 agattgcgac gcagcaggtc gtagaccgcg gcgtgaaaat cgggcaactg ctcggggcgt
     2701 aggcgcatca gcgcgacgtc gggcatcagc cgcaggatta ccacgccgac gatgcccagc
     2761 gtgtcggtgt cgcgcatcag cgccgcgagc gactcggctg ccagcgcctc attggcactg
     2821 ccggcgcgta gttcgagtgg ttcaagcaac aaggtagtaa tcccgcgggt cgatgccgta
     2881 ggcgttgccg gccagcgcac ggacgatgcg gtaggcctcg ccggtaggcg tcttcggttg
     2941 catcagcaag tcgagcgtgc gcggatggcg ttcgacgctc ttatctgggc cgagcagcac
     3001 catcaccttg ggctgcaggc ggcggacctg attctgctgg atgacgacgg cgacttcgcc
     3061 gctgttgaga tcgaccagcg taccgatcgg gtagaggccg atgcattgca cgaactggtc
     3121 aaccagcgtc tcgcggaagg ccttgccgcg catgcggttc agttcctcga gcacgtgctg
     3181 gttagagagg ccatcgctgt agggccgggc gcgcagcatc gcgcagtagg tatcgagcat
     3241 gccggccatt ccggcatgca gcgacagcac ctcgccagca atgccgcgcg gatagcccga
     3301 gccgtcgtgg cgctcgtggt ggttggcgac gatgtcgaaa agcgagggcg agaatacggc
     3361 tgtcttgcgc agcatgtcga gcgaactggc gacgtgggcg cggatctgac tccgctcgtc
     3421 gtcgtcgagc gcgccggtct tctgcagcag gatcggatcg acttcgctct tgccgatgtc
     3481 ctgcagcatg ccggcgagcc caaggctctc gaccgagtgc tgcggatagc cgagaaagga
     3541 ggcgaagacc atcatgtgta ccgacacgtc gatcgcgtga tcgtaggagt agctgtcggt
     3601 ttccttgagg cgcgccagcc acagcagtgc gtcggtattg cgggcgaccg actgcgccat
     3661 ctccatcgtc agcgaggcga gttcgacgaa atcgggtttc acgccgcttt cgatcgaggc
     3721 gaagatcgca ttcatcaccg actgcacgtc gtcgacgatg ctcgccgagt agatcatctc
     3781 ggcgacgagc tggctttgcc cgtcggacgg attgattacc ggtggggtgc cgcgcccttt
     3841 aggcagcttg cgattgcgga ccaggtaggc tgccgaatag aagtcgtcgt tctcgatcgc
     3901 tacctcgggt tcttcggcga aatcgtgcgg cttcgcgggc tgctcgtcgc gctcgtaggc
     3961 ggtgaagtcg acgcggcggc agtgcgagcg tgccggatcg acggtcacca ccttgcacag
     4021 tcggcgcagt tcctcgatct ggtcggcgcc gtcgacgtgg aaaccctgca gcagaaaggg
     4081 cgtacccagc caagggcggt caaggtcggt gacgaacatt ccgggaacga gatcggcgac
     4141 cgggacgatg tatttcttca tggtgtacct gacgctcctt tggctgtgtg cccgtcacgg
     4201 cgcatgcgct cgcgcctgtc ttttcccgat gatagcggca ggcgccgcca cccggacgga
     4261 attcgtcaca cccgcgccga tataatcggc cgccatggcg acgctccaaa ccgcggcgcg
     4321 gcactggaat cgagagactg gaggcggcca atgaagtata gagatctgcg cgagtttctc
     4381 gtcgggctgg agaaacgcgg cgaattgcgg cgggttggcg ttgccgtcga tccgcatctg
     4441 gaaatgaccg aaatctgcga tcgcacgctg cgcgtcggcg gaccggcggt gcttttcgag
     4501 cagccgacgg gccactcgat gccggtgctc ggcaacctgt tcggcacgcc cggccgcgtc
     4561 gcgctgggca tgggcgagga gcccgaaggt tggcagacct cgttgcgcga gatcggcaag
     4621 ctgctcgcct atcttaagga acccgagccg cccaagggcc tcaaggacgc ctgggaaaag
     4681 ctcccggtgc tgaaaaaggt gctcgacatg gcaccgagcg tgaagagttc ggcggcctgc
     4741 caggagatcg tctgggaggg cagcgaggtc gacctgtcgc gcctgccgat tcagacttgc
     4801 tggcccgggg atgcggcgcc gctgattact tgggggttga cggtgacgcg tggcccgcac
     4861 aaaaagcggc agaacctcgg tatctaccgg cagcaggtga tcgcccggaa caaggtgatc
     4921 atgcgctggc tggctcatcg cggcggcgcg ctcgattttc gcgaccactg cgtggcgaat
     4981 c
//
LOCUS       DATDAA010000348         5534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_480205,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000348 DATDAA010000000
VERSION     DATDAA010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480205"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VIS73_10505"
     CDS             complement(<1..192)
                     /locus_tag="VIS73_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEY5763624.1"
                     /translation="MTYQTTLTVATRGRGMTDLSDQVAAAVRAAGIDIGIVQVFVRHT
                     SCSLTITENADPDVRRDLET"
     gene            complement(239..670)
                     /locus_tag="VIS73_10510"
     CDS             complement(239..670)
                     /locus_tag="VIS73_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763625.1"
                     /translation="MGMLDRLFGSKPDYPMLPTDNPARARLDGMRGPLEDLAQRVSDP
                     LEVVPADDEAFVFLGKPPKRFGIAWLHDGKVVGIKELMEEHHLSATDANRLISQIGDA
                     YAAAEGAPRYAADIAGKTVVVIPSDDLEHQVHDLVEAATHH"
     gene            844..1422
                     /gene="cobU"
                     /locus_tag="VIS73_10515"
     CDS             844..1422
                     /gene="cobU"
                     /locus_tag="VIS73_10515"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEY5763626.1"
                     /translation="MVELILGGARSGKSRLAEQRALDWQASGRRVVYVATAQAGDAEM
                     AARIAQHRAGRPAEWRTVEVTLTLAETLRQEAAADSGVVVDCLTLWLSNLFFAGRAAA
                     QAEAGERIACELLEREVDALAATLPRLAGEVVLVSNEIGCGVVPMNAVARSFADRQGW
                     LNQQIAGLAARVTLVAAGLPLALKQQATGRGV"
     gene            1500..1952
                     /locus_tag="VIS73_10520"
     CDS             1500..1952
                     /locus_tag="VIS73_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY5763627.1"
                     /translation="MNSLFKFLHLASVVVWVGGMFFAYLCLRPVAAAQLQPPLRLALW
                     AGVFARFFPWVWAAVVLIVVTGLSTMLAIGFAAAPLHWHLMLTLGLVMSAIFAYVYFR
                     PYGALRAAVAAQNWPAGGAALGTIRQLVGVNLAIGVLTILSATVGRLL"
     gene            1952..2836
                     /locus_tag="VIS73_10525"
     CDS             1952..2836
                     /locus_tag="VIS73_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEY5763628.1"
                     /translation="MRWRAAVALALAGLGGAALAQPVSVVDDTGATVSLARPAARIVA
                     LTPHIVENLYAVGAGGQIVGAVEFSDYPSAARAIPRIGSYERFDVERIVALAPDLVIG
                     WESGNNRAQLAQLQAMGLTVYRSQPDTIDDVADELAAFGRLSGNAASGARAAEDYRGR
                     AAILRARFGAKARVPVFYQIWDSPLMTVGRRQIIGDVIDLCGGENVFRDLETMAPQVS
                     VEAVLAADAEVFVAGGMGEVRRDWLENWRRWSQLKAVQRDNLFFVPADWMQRHTSRLL
                     DGADLLCAHLELARSRRP"
     gene            3027..3815
                     /locus_tag="VIS73_10530"
     CDS             3027..3815
                     /locus_tag="VIS73_10530"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5763629.1"
                     /translation="MLALPALAAPYAFGVVPQRSAVLTAQYWNPILNYISTATGIQIE
                     LKMARTAPEHAEGIGRGEYDLLYSNHQFKPENDTQGYRVFARPIDASISGQIVVPADS
                     QLQKIEDLAGKEVVFPSQVAFVGFTVPMDGLKRAGIEVKALFAGNQEGAMGQLRAGRA
                     VAAGVNSQVMADYAKREAYGYRVLWASESYLNIPLAAHPRVPAAHVEALRKAFVAMSE
                     DPAGQLVLRTSAALVKQSPPYGFVVASDSEYDNQRRVYRGAVSR"
     gene            3826..>5534
                     /locus_tag="VIS73_10535"
     CDS             3826..>5534
                     /locus_tag="VIS73_10535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5763630.1"
                     /translation="MATIASGVWQRLSLTARLLIGCGLALGVYGATLLYTSISREVDN
                     SRAARDEQLQNELALVALTVTEQAIIGDYASLRQMLDARVSRAGDIVSIIWRDSRGNP
                     VAVESPREAVIAPPWFVAALGLRDVEGSLPIVVGGVSYGEVSLRIATSASVNWIWRNA
                     KDELQMLLIATGTIFGFIILIVTGALKPLGELAIFARRFGEGDVTVRVPDSATPELAP
                     TVDALNRMADGLNVVLISLDRARTTLAQEKEKAEVTLASIADAVLTTDVEGRVEFANA
                     VAQRLCGCTRAACEKLPIDAVVDLRDENGSVVGRLVDEVLVGGRPYAGSGHHVLVSRL
                     GERTAVEFSAAPIRGRDGTQVGAVIVIHDVSQTHRMATELSWQATHDSLTSLVNRREF
                     ERRLELMVERVRHGNSEHCLLYIDLDQFKVVNDTCGHVAGDELLRQVSTVLEDQIRES
                     DTLARLGGDEFGVLLENCPIAQGEHVAEKMRDALADFRFAWQTNTYQIGLSVGLVPIN
                     DAAQTTIGILSAADAACYSAKERGRNRVQVYRPNDSELAQRQGEMQWVAKITKAIEED
                     RLCL"
BASE COUNT          836 a         1782 c         1916 g         1000 t
ORIGIN      
        1 cgtctcgagg tcgcggcgaa cgtcggggtc ggcgttctcg gtgatcgtca gcgagcaact
       61 ggtgtggcgg acgaagacct gcacgattcc aatgtcgatg ccggccgcgc gaacggcggc
      121 tgcgacctga tcgctcaagt cggtcatgcc gcggccgcgt gtggcgaccg tcagggtggt
      181 ctggtaggtc attgcgcaga gtctacccgg tcatgggcgc cggggactct gcgttgtttc
      241 agtggtgagt ggccgcctcg accaggtcat ggacctggtg ttcgagatcg tccgatggaa
      301 tcaccacgac ggtcttgccg gcgatatcgg cggcgtagcg cggcgcgccc tcggcagccg
      361 cgtaggcgtc gccgatctgg ctgatcaggc ggttggcgtc ggtggccgac aggtgatgct
      421 cttccatcag ctccttgatg ccgacgactt tgccgtcgtg caaccaggcg atgccgaagc
      481 gctttggtgg cttgccgaga aagacgaagg cctcgtcatc ggccggaacc acctcgaggg
      541 gatcggatac gcgctgggcg agatcctcga gcgggccgcg catgccatcg aggcgggcgc
      601 gggcggggtt gtcggtgggc agcatcgggt agtcgggttt gctgccgaac aggcgatcaa
      661 gcattcccat ggtttcctcc tttccagcgt gtgacggaag gttccggctg ccagtatgtg
      721 ccgccaaaac ccccctaagc ctaggacacg ctggacggca ttgccaatgc ccctggaata
      781 gagcgccggc gcgcgggcga ttcggtcacc cgcccaggtg ccgaatcgct atcatcggca
      841 gccatggtcg aactgatatt gggcggtgcc cgctcgggaa aaagccgcct cgccgagcag
      901 cgcgcgctgg actggcaggc gagcggtcgt cgcgtcgtct atgtggcgac ggcacaggct
      961 ggcgatgccg agatggccgc gcggatcgcg cagcaccgcg ccgggcggcc cgccgagtgg
     1021 cggaccgtcg aggtgacgct gaccctggcg gaaacgctgc ggcaggaggc agccgcagac
     1081 agcggcgtcg tcgtcgactg cctgacgctg tggctctcca acctgttctt tgccggtcgc
     1141 gccgcggcgc aggccgaggc cggcgagcgc attgcctgcg aactgctcga acgcgaggtc
     1201 gatgcactcg ccgcgacctt gccgaggctt gccggcgagg tcgtgctggt cagcaacgaa
     1261 atcggctgcg gcgtcgtgcc aatgaacgcg gtggcgcgtt cgtttgccga ccgccagggc
     1321 tggctcaacc agcaaatcgc cggcctcgcc gcaagggtga cgctggtggc ggcgggcttg
     1381 ccgctggcgc tgaagcagca ggcaaccggg cgcggagttt gagttttgcc gaggagttgc
     1441 ggccgacgcg gtaggattcg cgcgtctgcc tctatcaacg cttcccggag tgcccgtcga
     1501 tgaattccct gttcaagttt ctgcatctcg cctcggtcgt tgtctgggtc ggcggcatgt
     1561 tcttcgccta cctttgcctg cgcccggtcg ccgctgccca attgcagccg ccgttgcgac
     1621 tcgcgctgtg ggccggcgtc ttcgcgcgct tcttcccgtg ggtgtgggcg gctgtggtgc
     1681 tgatcgtcgt taccggactg agcacgatgc tggcaatcgg tttcgccgcc gcccccttgc
     1741 actggcatct gatgctgacc ctcggcctgg tgatgagcgc gatcttcgcc tacgtgtatt
     1801 tccgccccta cggtgcgctg cgcgctgcgg tcgctgcgca gaactggccg gccggtggcg
     1861 cggcgcttgg cacgattcgc cagctggtcg gtgtcaacct ggccatcggc gtgctgacca
     1921 tcctcagcgc gaccgtcggg cgcctgctct gatgcgctgg cgcgccgccg tcgcgctggc
     1981 ccttgcgggc cttggcgggg cggcactggc gcagccggtc agcgtcgtcg atgacaccgg
     2041 cgcgaccgtg tcgctggcgc gaccggccgc gcgcatcgtc gcgctgaccc cgcacatcgt
     2101 cgagaacctc tacgcggtcg gtgccggcgg gcagatcgtc ggcgccgtgg agttcagcga
     2161 ttacccgtcg gcggcgcgcg cgattccccg catcggcagt tacgagcgct tcgacgtcga
     2221 gcgcatcgtc gcgcttgccc cagatttggt catcggctgg gagagcggca acaaccgcgc
     2281 ccagctggca cagctgcagg cgatgggtct tacggtctat cgctcgcagc ccgacacgat
     2341 cgacgatgtc gccgatgaac tcgcggcttt cggcaggctg tcgggcaatg cggcaagcgg
     2401 cgcgcgtgcc gccgaggact atcgcggtcg cgccgcgatc ctgcgcgcgc gcttcggcgc
     2461 caaggcgcgg gtgccggtgt tctaccagat atgggattca ccgttgatga cggtgggccg
     2521 gcggcagatc atcggcgacg tgatcgacct gtgcggcggc gagaacgtct ttcgcgatct
     2581 cgaaacgatg gcgccgcagg tatctgtcga agcggtgctc gccgccgacg ccgaggtctt
     2641 cgttgccggc ggtatgggcg aggttcgtcg cgactggctg gagaactggc ggcgctggag
     2701 ccagctaaag gccgtgcagc gcgacaatct gttcttcgtc ccggctgact ggatgcagcg
     2761 ccacacgtcg cggctgcttg acggcgccga cctgctttgc gcgcatctgg agctggcgcg
     2821 cagtcgccgg ccgtaaagcg gcgtatcgcg gtagccgtcg cttctcccgc ccgccggttg
     2881 agtatgctac ggtcacgccg ggatttgatc cgcgcttcat tggacgaggg aggggatcca
     2941 tggcaatcac ggcgtgcttg tgcccagctg gttttcgaag acggtggcgc cgcctatctt
     3001 cactgggggc cgccggcttc ttcgcgatgc tggcactgcc ggcgctggcc gcgccctatg
     3061 ccttcggcgt cgtgccgcag cgcagcgccg tgctcacggc acagtactgg aaccccatcc
     3121 tcaactacat cagcacggca accggcatcc aaatcgaact gaagatggcg cggacggcgc
     3181 ccgagcacgc cgaaggcatc ggccgcggcg aatacgacct gctctattcg aatcaccagt
     3241 tcaagccgga aaacgacacg cagggctacc gggtcttcgc gcgaccgatt gatgcgtcga
     3301 tttccgggca gatcgtcgtc cccgccgatt cgcaattgca gaagatcgag gatctagccg
     3361 gcaaggaggt ggtgtttccc tcgcaggtcg ccttcgttgg ctttaccgtg cccatggacg
     3421 gcctgaagcg ggccggcatc gaagtcaagg ccctgttcgc cggcaatcag gaaggcgcaa
     3481 tgggccagtt gcgcgccggc cgggcggtcg ccgccggcgt caattcgcag gtgatggccg
     3541 actacgcgaa gcgcgaggct tacggttacc gcgttttgtg ggcttccgaa agctatctga
     3601 acattccgct ggccgcccat ccgcgggttc ccgctgcgca tgtcgaggcg ttgcgcaagg
     3661 ccttcgtcgc gatgtccgaa gatccggcgg ggcagctcgt tctgcgcacc agtgcggcct
     3721 tggtcaagca atcaccgccc tacggtttcg tcgtcgccag cgattccgag tacgacaacc
     3781 agcgtcgcgt ctatcgcggc gccgtctcac gctaggcagg tatcagtggc gacaatcgcg
     3841 tcgggagtct ggcagcggct cagtctcacg gctcggttgc ttatcggttg cggtctggcg
     3901 ctcggcgtct acggcgcgac gctgctctat acctcgatca gccgcgaggt cgacaacagc
     3961 cgagccgcac gcgacgagca gctgcagaac gaactggccc tcgtcgcgct gaccgtgacc
     4021 gagcaggcga tcatcggcga ctacgcctct ttgcggcaga tgctcgacgc gcgcgtctcg
     4081 cgcgccggcg acatcgtctc gatcatctgg cgagactcgc gcggcaaccc ggttgcggtg
     4141 gagagcccgc gcgaggccgt gatcgcgccg ccctggttcg ttgcggcgct tggcctgcgc
     4201 gatgtcgagg gttcgctgcc gatcgtcgtc ggcggcgttt cctatggcga ggtatcgctg
     4261 cgcatcgcga ccagcgccag cgtcaactgg atctggcgca acgccaagga tgagttgcag
     4321 atgctgttga tcgccaccgg tacgattttc ggtttcatca tcctcatcgt taccggtgcg
     4381 ctgaagccgc tcggtgagct ggcgatcttc gcccggcgct tcggcgaggg cgacgtgacg
     4441 gtccgcgttc ccgactcggc gacgccggag ctggcgccga ccgtcgacgc gctcaaccgc
     4501 atggccgatg gccttaacgt cgtgctgatc tcgctcgacc gcgcgcgaac gactctggcg
     4561 caggaaaagg agaaggccga ggtaacgctg gcgtcgatcg ccgatgcggt cctgaccacc
     4621 gacgttgaag gccgggtcga attcgccaac gcggtcgccc agcgcctttg cggttgtacg
     4681 cgggcggcct gcgagaagct gccgatagac gccgtggttg acctgcgcga cgaaaatggt
     4741 tcggtggtcg gacggctggt cgacgaggtg ctggtcggtg gtcgccccta cgcggggagc
     4801 ggtcaccacg tcctcgtctc gcgtctgggc gagcggacgg ccgtcgagtt ctcggccgca
     4861 ccgattcgcg gccgcgacgg cacccaggtc ggcgcggtga tcgtcatcca cgacgtcagc
     4921 cagacccatc gcatggcgac cgagttgtcc tggcaggcca cgcacgattc gctgaccagc
     4981 ctagtcaacc ggcgcgagtt cgagcggcgg ctcgaactca tggtcgagcg ggtgcgccac
     5041 ggcaacagcg agcactgcct gctctacatc gacctcgacc agttcaaggt ggtcaacgac
     5101 acctgcggtc acgttgccgg cgacgagttg ctgcggcagg tgtcgactgt gctcgaggat
     5161 cagattcgcg agagcgacac actcgcccgc ctagggggcg acgagttcgg cgtgctgctc
     5221 gagaactgcc cgattgctca gggtgagcac gtcgccgaga agatgcgcga tgcgctggcc
     5281 gacttccgtt tcgcctggca gacgaatacc taccagattg gtctctcggt cggcctagtg
     5341 ccgatcaacg acgcggcgca gacgacgatc gggattctgt cggcggccga cgccgcctgc
     5401 tactcggcca aggaacgcgg gcgcaaccgg gtccaggtct accggcccaa cgacagcgag
     5461 ctggcgcagc gccagggcga gatgcagtgg gtggccaaga tcaccaaggc aatcgaggaa
     5521 gaccggctct gcct
//
LOCUS       DATDAA010000349         4780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_95542,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000349 DATDAA010000000
VERSION     DATDAA010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95542"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VIS73_10540"
     CDS             complement(<1..265)
                     /locus_tag="VIS73_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763631.1"
                     /translation="MPIFRATAIWFLILVLAIANGAFRESVLIPALGVPSAPMLSGLV
                     LGSVIFLCAWFLLPRPLPPRQAWAIGAWWLGMTLVFEFSFGRLV"
     gene            complement(378..785)
                     /locus_tag="VIS73_10545"
     CDS             complement(378..785)
                     /locus_tag="VIS73_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HEY5763632.1"
                     /translation="MAQVVFYEKPGCTGNSQQRAMLVAAGHELDVRDLLRTPWTAETL
                     HPFFGGLPVVQWFNPRAPAVRDREIDPSQLDEAAALAAMIAAPILIRRPLLQVGDERR
                     CGFDPKTINRWIGLLAADARRIDAEACQSTRCP"
     gene            complement(821..1384)
                     /locus_tag="VIS73_10550"
     CDS             complement(821..1384)
                     /locus_tag="VIS73_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763633.1"
                     /translation="MTTPLFSRLEGKLLSPTFSGPTQKANRYGFRGELGLKFPPSVAG
                     EKRPPEIKADQVILAAEGESLVFLALHVDSLAYLDHVRETFGDALVAGGKYFIFAGNV
                     DISKKFSLELDGVTFFVLPLDEATVYNEMLDLLYLERGDLKRVPNEGKIDAIVDAAAS
                     VSGFSAMPYTQALAEMGPVKVADNRPV"
     gene            1630..1944
                     /locus_tag="VIS73_10555"
     CDS             1630..1944
                     /locus_tag="VIS73_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HEY5763634.1"
                     /translation="MQPQPATPNYAYFRGDLVYAATDLYNDGPQADGSSGLPEAKPGA
                     LLAAAGTRGMVVNVGHPERAPGEVIYLVAFEGAEDRELGLPFGCLPEELTQDESRVGA
                     GS"
     gene            1941..2192
                     /locus_tag="VIS73_10560"
     CDS             1941..2192
                     /locus_tag="VIS73_10560"
                     /inference="COORDINATES: protein motif:HMM:NF022143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HEY5763635.1"
                     /translation="MTQTDPQSIHGHVILRQLAETPMTRTAIAARARHDFGAGARFHT
                     CDTQGLSFDALFDLLAARGKIVADGELWATETAKVCADE"
     gene            complement(2218..2625)
                     /locus_tag="VIS73_10565"
     CDS             complement(2218..2625)
                     /locus_tag="VIS73_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5763636.1"
                     /translation="MRPRISMITLGVRDVSRATAFYETGLGWPRMHSPPEVAFFTLNG
                     SWLGLYGRDDLAGDAGVPAEGSGFPGFALAHNVDSEAEVDALLLQAAKAGGTIVRPAH
                     KTTWGGYAGYFADPDGYLWEIAHNPLFWVGPRD"
     gene            complement(2856..3485)
                     /locus_tag="VIS73_10570"
     CDS             complement(2856..3485)
                     /locus_tag="VIS73_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763637.1"
                     /translation="MRRSILLLCTTALLGACASPPIKDPASRYFDPPSGSQVVVRQAI
                     DIAAGYAHAVIQDGKSVTPSALRRYEPFCEIEVNDVLQRDQQVGPGDFAITRVIRQQQ
                     QVGLVPTNRYAGRDEPLRLAGVGFGIGVGSGGGFGWSLGIGIPIGANDRESGPLVFNL
                     VSMRLHSAAQPNVRELRCSGGWASISEVVYPTLAEMNKALGALAEIRLP"
     gene            3779..>4780
                     /gene="nifB"
                     /locus_tag="VIS73_10575"
     CDS             3779..>4780
                     /gene="nifB"
                     /locus_tag="VIS73_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HEY5763638.1"
                     /translation="MTAPAASPTSRKVIPLMLAGAKPAGPCSSAAKGNCGAGSSAQLP
                     QHIRDKVHDHPCYSAEAHNHFARMHVAVAPGCNIQCNYCNRKYDCANESRPGVVSEKL
                     SPELAARKVLAVAARIPQLSVVGVAGPGDALAIPERTFKTFELIQAAAPDIRLCLSTN
                     GLALPDHVERIKALGVDHVTITINMVDPEIGAQIYPWIFFDHKRRRGVEAATILHERQ
                     MLGLEMLVEAGILVKVNSVMIPGINDRHLVEANEAVKSRGAFLHNIMPLISDPAHGTV
                     FGLNGQRGPTPAELKELQDACEGDMQLMRHCRQCRADAVGMLGEDRGAEFSSDRIEAI
                     "
BASE COUNT          871 a         1487 c         1619 g          803 t
ORIGIN      
        1 cgacgaggcg cccaaaagag aactcgaaga ccagcgtcat tcccagccac caggcgccga
       61 tggcccaagc ctggcgcggt ggtaacggtc gcggcagtag gaaccaggcg cagaggaaga
      121 tcacgctgcc gagtaccaga ccgctgagca ttggcgccga cggaacgccc agcgccggga
      181 tcagaacgct ttcgcggaaa gcgccgttag cgatggcgag gacgaggatc aggaaccaga
      241 tggcggtggc gcggaatatg ggcatagcgc tggtgtcggc gatattgcgc cagcataggc
      301 ggtggtgggg ttgccgtcca tcggcgaggc ggcgcgactc tcagctcgcc gccttgtcgt
      361 gggttgcgtc tggcccgcta cgggcagcgg gtcgactggc acgcctcggc gtcgatgcgc
      421 cgggcgtcgg cagcaagcag gccgatccag cggttgatcg tcttgggatc gaaaccgcag
      481 cgtcgttcgt cgccgacctg taacagcggc cggcggatca gaatcggggc ggcgatcatc
      541 gccgcgagtg ctgcggcttc atcgagctgc gacggatcga tttccctgtc gcgcacggcc
      601 ggcgcgcgtg ggttaaacca ctgcacgacc ggtaggccgc cgaagaatgg gtgcagcgtc
      661 tcggcagtcc acggcgtgcg caacaggtct cgtacgtcga gttcgtgccc ggccgcgacc
      721 agcatcgcgc gttgttgcga gttgccggtg cagcctggtt tttcgtagaa gacgacctgt
      781 gccatggccg ccgaggcttg ttggattgca agccgcgcaa tcagaccggg cggttgtcgg
      841 cgaccttcac cgggcccatt tcggcgagcg cttgggtgta gggcatcgcc gagaatccgg
      901 acaccgaagc ggcggcgtcg acgatcgcgt cgatcttgcc ctcgttggga acacgcttga
      961 gatcgccgcg ctcaaggtag agcaggtcga gcatctcgtt gtagaccgtg gcctcgtcaa
     1021 gcggcaggac gaagaaggtg acgccgtcga gctccagcga gaactttttc gaaatgtcga
     1081 cgttgccggc aaagatgaag tacttcccgc cggcgaccaa ggcgtcgccg aaggtttctc
     1141 ggacgtggtc gagataggcg agcgaatcga cgtgcagcgc gaggaagacc agcgattcgc
     1201 cttcggccgc gaggatcacc tggtcggctt tgatctccgg cgggcgcttc tcgccggcaa
     1261 ccgagggggg aaacttaagg cccagttcgc cgcggaatcc gtagcggttg gccttctgcg
     1321 tggggccgct gaaagtgggg gagagcagct tgccttcgag gcgggaaaag agaggcgtgg
     1381 tcatatcggg tctcgagtct ggttgattaa cgctgagcca aacgcgagaa gcgtgccagt
     1441 cacccaagtg gctgatcgca agcgatattc tcgaaatcag cgcagggcgg tgcgacgcct
     1501 tgtccggttc acgacacggt tttgcgacac gatgatcggg cgaccggcaa ctctccgccg
     1561 agacgacggt gatagcggcg ttggcgcgtc gcttgcatgg gttgtcgtgc cctgccaatg
     1621 gagagcccca tgcagcctca gccagcaacg ccgaactatg cgtattttcg cggcgatctc
     1681 gtctatgccg cgaccgatct gtacaacgat ggcccgcaag ccgacggcag cagcgggcta
     1741 cccgaggcca agccaggcgc gctgctcgct gcggcaggca cgcgcggcat ggtcgtcaac
     1801 gtcgggcacc ccgagagggc accgggcgag gtgatctacc tcgttgcgtt cgaaggcgcc
     1861 gaggatcgcg aactcggact ccccttcggc tgcctgcccg aggaactgac gcaggacgag
     1921 tcccgcgttg gagccggctc atgacgcaga ccgatccgca gtcgatccac ggccatgtga
     1981 ttctccgcca actggccgaa acgccgatga cgcggacggc gatcgccgcc cgcgcacgac
     2041 acgatttcgg cgcgggggcg cgttttcaca cctgcgacac gcagggcttg tccttcgacg
     2101 cattgttcga cctgctcgcg gcgcgcggca agatcgtcgc cgatggcgag ttgtgggcaa
     2161 ccgagacagc gaaggtctgc gccgacgagt gaatcgccgg caccgatatc gacgacctca
     2221 gtcgcgtggg ccgacccaga acagcgggtt gtgggcgatc tcccagagat agccgtcggg
     2281 gtcggcaaag taaccggcat agccgcccca ggtggtcttg tgcgccgggc ggacgatcgt
     2341 cccgccggcc ttcgcggcct gcagcaacag cgcatcgacc tcggcttcgg agtcgacgtt
     2401 gtgggccagc gcgaaacctg ggaagccgct accttcggcc gggacacccg cgtcgcctgc
     2461 gaggtcatcg cgtccgtaca ggccgagcca ggaaccgttc agtgtgaaga acgcgacctc
     2521 gggcggcgag tgcatgcgcg gccagccgag cccggtctcg tagaaggcgg tggcgcgtga
     2581 aacgtcgcgc acgccgagcg tgatcatgct gatgcggggt ctcatgtggt gaggcctccc
     2641 ggtggacagc ctatcgctcc cgcgccagaa atcgcgaagc agtaacgtgg ggctttgatg
     2701 tcgagccgtg acgtcgtgag caatgtattc atcgccgtac tgcgggtcgc gctgcatctc
     2761 cttcgcacaa ggacgatctc gcacggcggc gcggtcgatc catgcgtttg cggagccgca
     2821 agccgtgggg gcaggcgctt atccaatttg gtgccctacg gcagacgaat ttcggccagt
     2881 gcgccgagcg ccttgttcat ctcggcgagc gttgggtata cgacttccga tatcgaggcc
     2941 cagccgccgc tgcagcgcag ttcgcggaca ttgggctggg ccgcggaatg caggcgcatt
     3001 gacacgagat taaagaccag cgggccgctt tcgcggtcgt tggcaccgat cggaatgccg
     3061 atgccgaggc tccagccgaa gccaccgcca ctgccgacac cgattccaaa cccgacgccg
     3121 gcgagtcgca gcggctcgtc gcgtcccgcg tagcggttgg tcggcacgag gccgacctgt
     3181 tgctgctggc ggatgacacg ggtaatcgcg aaatcaccgg gaccgacctg ctggtcacgc
     3241 tgcaggacgt cgttcacctc gatctcgcag aacggctcgt agcgtcgcag ggcgctgggt
     3301 gtcaccgact tgccgtcctg gattaccgca tgggcgtagc cggcggcgat atcgatcgcc
     3361 tggcggacga ccacctggct gccgctcggc gggtcaaagt agcgcgaagc cgggtctttg
     3421 atcggcgggc tggcgcaagc gccgagcagg gcggtggtgc aaagcagcag gatggagcgt
     3481 cgcatggtgt tctccgcggg ccggagtgtc ctcgtcggcg atcgccgacg aggcaggcca
     3541 cctgaatcag accgccggcg ccagtttcgg tttccaccgg ccgatcgatg tcgcttgtcg
     3601 tgatcgcccc gtaattgttg caaagcggac agcggtgtct ggttaacgga acgagtataa
     3661 aacgcttaca aaacagttgc atagcgcgct acaaggcatg tggcacgggc gttgcagtag
     3721 gcagtgcgag acgaagccgg catcgccctt cgtcgccagc cactgaccgg agtccgtcat
     3781 gacagcgcct gcagcgagcc cgacatcccg caaggtcatc cccctgatgc tggccggcgc
     3841 gaagccggcg ggcccgtgtt ccagcgccgc caagggcaac tgcggcgccg gcagctcggc
     3901 gcagctgccg cagcacattc gcgacaaggt gcacgatcat ccgtgctaca gcgccgaggc
     3961 gcacaaccac tttgcgcgca tgcacgttgc cgtagcgccc ggctgcaaca tccagtgcaa
     4021 ctactgcaac cgcaagtacg actgtgccaa cgagtcgcgt cccggcgtcg tgtcggaaaa
     4081 actctcgccc gaactggcag cgagaaaggt gctggcggtt gccgcgcgga ttccgcagct
     4141 gtcggtggtt ggcgtcgccg ggcccggcga tgcgctggcg attcccgagc ggacgttcaa
     4201 gaccttcgaa ctcatccagg ccgccgcgcc ggacatccgc ctttgcctgt cgaccaacgg
     4261 actggccttg cctgatcacg tcgagcggat caaggcgctc ggcgtcgatc acgtgactat
     4321 caccatcaac atggtcgatc ccgagatcgg cgcccagatc tacccgtgga tcttcttcga
     4381 tcacaagcgc cgccgtggcg tcgaagcagc gacgattctt cacgagcggc agatgctcgg
     4441 actggaaatg ctggtcgaag ccgggattct ggtgaaggtc aattcggtca tgatcccggg
     4501 catcaacgac cgccatctgg tcgaagccaa tgaggcggtc aagtcacgcg gtgcctttct
     4561 ccataacatc atgccgctga tctccgaccc ggcgcacggg acggtcttcg ggctgaatgg
     4621 gcagcgcggt ccgacgccgg cggaactcaa ggaattgcag gacgcatgcg aaggcgacat
     4681 gcaactgatg cgccattgtc ggcaatgccg cgccgacgcc gtgggcatgc tcggcgaaga
     4741 tcgcggcgcg gaattctcca gcgaccggat cgaagcaatc
//
LOCUS       DATDAA010000350         2547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_305808,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000350 DATDAA010000000
VERSION     DATDAA010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305808"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VIS73_10580"
     CDS             <1..438
                     /locus_tag="VIS73_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763639.1"
                     /translation="ISCYTALGDTEGSRRAAELALQRAEAVLAHDQNNSMVAGYSAYA
                     LAALGEAERAKARMERALLIDPDNFNMRYNFGCALCMYLQDKEAALDMLGPIFDTITD
                     TFLPYAKADPDLELLRDDPRYAAMVAAAEARLANANDPQATES"
     gene            617..2449
                     /locus_tag="VIS73_10585"
     CDS             617..2449
                     /locus_tag="VIS73_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763640.1"
                     /translation="MSFFAELKRRNVFRVGVAYILAGWVVLQAADFALDLVDAPNWVI
                     QVFVIAVAAGLPLALLFAWAYEMTPAGIRREKEVDRSQSITRNTGRKLDRTIIGLLVL
                     AIVVLGVERVFFAGEPAADAEATAAAGVDTPKSIAVLPFTDMSQAQDQEWFADGLAEE
                     ILNALVKVPDLRVAARTSSFQYKGSGLAIGDIASELGVAHVLEGSVRSGADRIRVTAQ
                     LIRASDGFHLWSQNYDRDVDDMIGIQEDLAVNIANALETSMDPQALAEMARVGTASVD
                     AYREYLRGVQLVLEAVNISHGAEEQRAAYEHFERARSLDPGFADAHFQAANYWIVELS
                     PSRTDTGSSGLTPGESLREYHDRIDQAIDNAKTEADRLRSLASRAVVDLQLREARRLY
                     EQYLDMRPNDDPARAEAMGILDWLSEDAAAEAELGRAAALGQTSLFWASNYIGAAYTV
                     ADPAEAADVGLALLERWPNSSGMVYQVHRTLLWAGRFEEASRLAARYNTLVPGGSLLV
                     AAREACAAGDRATAEKILEQLEEEEDPNVSQLWILHNMLGNTVEETEVLRPLEQNGVP
                     YQLASFLTYHKFDPRPVPELMAILEREGVERPEPAVPPFRCPPR"
BASE COUNT          460 a          796 c          827 g          464 t
ORIGIN      
        1 attagctgct acacggcact gggagatacc gagggaagcc gtcgcgctgc ggagctggca
       61 ctccagcgcg ccgaggccgt cctggcgcat gaccagaata attccatggt ggcaggttac
      121 agcgcttacg ctttggcggc gctcggagag gcagaacgcg ccaaagcgcg catggaacgc
      181 gcacttttga ttgatccaga caatttcaac atgcgctaca acttcggctg cgccttgtgc
      241 atgtatctgc aggacaagga agccgcactg gacatgcttg gccctatttt cgacacgatc
      301 acggatacct tcctgcctta tgcaaaagcc gatccggacc tcgagttgct gcgcgatgat
      361 ccgcgctatg ctgccatggt cgccgcagcc gaagcgcgcc tcgcaaatgc aaacgacccg
      421 caggcgacag agtcctgaca gccgacacgc gcagccacgg ttcccctggt ctttgagtta
      481 actgttgcag tagagcatgc gtttccgggg atgcggtgcg tggcgcttac ccgtgttgct
      541 gccgggtccg aagagaaagc tcggctgtgc gctggccttt ttgctaacct tgcgcagcga
      601 tgatcgagga aacgcgatgt ccttttttgc ggagctgaag cgccggaacg tattcagggt
      661 aggggtcgcc tacatcctgg cgggctgggt cgtgctgcag gccgcggact tcgcgctcga
      721 cctcgtcgat gcacccaact gggttatcca ggtcttcgtt atcgccgtgg cggccggcct
      781 gccgctggcg ctcctgttcg cctgggctta cgagatgacg ccggcgggga tcaggcgcga
      841 gaaagaggtc gaccgcagcc agtccatcac gaggaatacg gggcgcaagc tcgatcgcac
      901 gatcatcggc ttgctcgtgc tagccatcgt agtgctgggc gttgaacgcg tgttctttgc
      961 cggcgagcct gcagccgatg ccgaagcgac cgcggcagcc ggggtcgaca ctcccaagtc
     1021 gattgccgtg ctgcctttta ccgacatgag ccaggcgcag gaccaggagt ggtttgccga
     1081 tgggctcgcc gaagagattc tcaatgccct cgtgaaggtc ccggacctgc gggtggcggc
     1141 ccgcacgtcc tctttccagt acaaggggtc cggcctggcc attggcgata ttgccagcga
     1201 gctgggtgtc gcccatgtgc tcgaagggtc cgtgagaagc ggcgcggatc gcatccgcgt
     1261 cacggcacag ctcatccggg ccagcgacgg ctttcacctg tggtcgcaga actatgaccg
     1321 cgacgtcgac gacatgatcg gcatccagga ggacctggcg gtcaatatcg ccaatgcgct
     1381 cgaaacctcg atggacccgc aggcgctcgc cgagatggcg cgcgtcggta cggcgtcggt
     1441 cgacgcctac cgtgaatacc tgcgcggcgt ccagctggtc cttgaggcag tcaatatttc
     1501 gcatggcgca gaagagcagc gggccgccta cgagcacttc gagcgggccc ggtccctcga
     1561 cccgggtttt gccgacgcgc attttcaggc ggcaaattac tggattgtag aactctcacc
     1621 gtcgcgcacc gatacgggtt cctcggggct gacaccgggg gagagcctgc gcgagtatca
     1681 cgaccgcatc gaccaggcga tcgacaatgc gaagaccgag gcggaccggc tgcgcagcct
     1741 ggccagccgg gccgtcgtcg acctgcagtt acgggaagcc cggcgtctct atgagcagta
     1801 tctcgacatg cggcccaatg atgatccggc ccgagccgaa gctatgggta ttctggactg
     1861 gttgtccgag gacgcggccg cggaagcaga actgggtcgc gcggcagcac taggtcagac
     1921 cagcctgttt tgggcctcca actacatcgg tgcagcctac acggttgccg accctgcgga
     1981 ggccgcggat gtcggcctgg cgctcctcga gcgctggccg aattccagtg gcatggtcta
     2041 ccaggttcac cggaccctgc tctgggccgg gcggttcgag gaggcgtcgc gccttgcggc
     2101 ccgctacaac acgctggtgc ccggcggcag tctgctggtg gcggccaggg aggcctgcgc
     2161 cgcgggtgat cgggccacgg ccgagaagat tctcgagcag ctcgaagagg aggaagaccc
     2221 gaacgtctcc caactctgga tactgcacaa catgctcggc aacacggtcg aggaaacgga
     2281 agtactgcgc ccgctggagc aaaacggcgt gccctaccag cttgctagtt ttctcaccta
     2341 ccacaagttc gacccgcggc cggtccccga gctcatggcg atactcgaac gcgaaggcgt
     2401 cgagcgaccg gaaccggccg tgccgccgtt tcgctgcccg ccgcgctgaa ccggcactcc
     2461 agtcgtcgcc ggatcgtagc gtgccgggct cggcaagtct gccgtcgcct ggcaatcctt
     2521 agagatgcca tgactcattc gacgggt
//
LOCUS       DATDAA010000351        10149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_340381,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000351 DATDAA010000000
VERSION     DATDAA010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10149
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340381"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VIS73_10590"
     CDS             complement(<1..530)
                     /locus_tag="VIS73_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY5763641.1"
                     /translation="MNTVADIKNHVVADLKLADWGRKEIRIAETEMPGLMAIREEFAK
                     AQPLKGARIAGSLHMTIQTAVLIETLQALGADVRWASCNIFSTQDHAAAAIAAAGTPV
                     FAVKGETLTEYWDYTHRIFEWADGGTPNMILDDGGDATLLLHLGARAEKDPSVLGKPA
                     SEEERVLFAAIKKRNA"
     regulatory      complement(607..690)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            1040..2527
                     /locus_tag="VIS73_10595"
     CDS             1040..2527
                     /locus_tag="VIS73_10595"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY5763642.1"
                     /translation="MAKVKLGGRLWLRAALSCLLSVVAVPVLAADQAERALAFVAELV
                     ARGEIRAGTTLRLAFKPGNINALLGADLELQREWEEKTGTRISARVMPNQPTLANFAS
                     YPDFDLTVGRPYEHQELLDKALIAPLDDLAARFGFAIDDHGASGFIHPRLQATFGGRL
                     LAIPADGDVTLLYLRHDLLDDPAEQKAYRARTGRSLAIPRTWAEYDAQVRFFHRPDKE
                     FYGAAEERDPEGGWMYWMQRYLPQQAPYQPLFDEQARPLIDSPAGVSATEHYVHSARH
                     GPPQATEEGRDYSYVLPLFMQGKAYSAMTTIAGAKLFTSAGSSVLGRFSAIPMPGVMV
                     DGRLVRRNMPIYGNNLMVAKASPNQALAFLFAMWLTDPVVSLRTVGARGGHTDPYRWH
                     HLTDPRIESIYTPAALSVFAREWDVAVPSGMGLIDESEYATVLDRQLWLAARGEITPA
                     EAMKRTAREWERLTERIGRDRQIASLQAFRAEMLRREPLPPGGGR"
     gene            2541..5078
                     /locus_tag="VIS73_10600"
     CDS             2541..5078
                     /locus_tag="VIS73_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5763643.1"
                     /translation="MNTRRRFGLVAQSLFGFAVGGVLVAILIFAGDRMLRTGIARLQA
                     TLDQQILPLADLQRLQSRLNYLRLDELEMLSVRDVFQLPTQAARMRGDLRVVDAALAE
                     FAERLGQNQPADSIRLRGHWQEYRGLLDGKLRHAEAMDIAAIQRESKTTSYLPYVAIE
                     SLLAEIAVAKRTAAEQAYQRTVDEQAGQRRVFMSLVLAGTLLLITVLAWSGRTVIRRI
                     GILNDSARRLASGGEGWHADVGGNDEISDLAEAFNRMREEVLARERALQEARDELENR
                     VVERTRELRDSNERLTLLSEVVEQNPVGILIADRSGRIDYVNPAYSTLTGQPASRLVG
                     QPLELLLAVDHMRAAAEATPAVLDGGPEWQDEQVSYRDDGGEYWERLRILAVRDANGN
                     SSHLLMIREDISERREQEERIAYQAHYDALTALPNRVLALDRLVQVTGQARRDGERAG
                     VMFLDLDNFKEINDTLGHEAGDELLQLAATRLRGAMREADTVARLGGDEFLVILAGLG
                     RGKDAAGVADKIIESFTAPFPVAGRDLVVSPSIGLSIFPDDGDEPAVLLRNADLAMYE
                     AKEAGRNTYRFFNQAIHDLSLRRLEIGRRLRLAMAGGELRLLYQPLIHAPSGRMIGAE
                     ALLRWDNDELGSVAPEIFIPVAEQGGLIVEIGGWVLREACARAAHWRTRQPDFLMAVN
                     VSPRQFRETDFAELVGRTLAEQKLPAAALEIEVTEGLLLHNQDDVKAVLSALRELGVR
                     LSMDDFGTGYSSLSYLRAFPFTTLKIDRGFVRDVSEDAGDRDLVIAAVGMAKALGLRV
                     IAEGVETDAQWMFLNGIDCDIVQGYRFGKPVSAQDFEATWLSGIREH"
     gene            complement(5128..6459)
                     /locus_tag="VIS73_10605"
     CDS             complement(5128..6459)
                     /locus_tag="VIS73_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5763644.1"
                     /translation="MAELARAVADEGYTTPTPIQTQAIPLILAGRDVLAAAQTGTGKT
                     AGFALPLLQRLASNPLKPAARRVRALVLTPTRELAAQVEEAIKTYGQHLSLKSTLVYG
                     GVGINPQIAALKPGVDVLVATPGRLLDHAGQRTVDLSAIEILVLDEADRMLDMGFIHD
                     IKRVLKLLPAKKQTLLFSATFSDEIRRLAEGLLDNPASVEVARRNADSELVSQVIHPV
                     AKNRKRELLRDLIADGRWEQVLVFTRMKHGANRLAEQLNKDGITAAAIHGNKSQGART
                     KALSGFKNGDIRVLVATDIAARGLDIEQLPHVVNYELPNVSEDYVHRIGRTGRAGSPG
                     HAVSLVSPDEMDFLRGIERLLKKRLHQVVMPGYDGPPPPAMVEADSRPPRPPGRGQSP
                     GRGNSQRRPQGGAASGKPKAGGSPAAKPAARPAAQRPASGAPALLARRRTP"
     gene            complement(6755..7429)
                     /locus_tag="VIS73_10610"
     CDS             complement(6755..7429)
                     /locus_tag="VIS73_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY5763645.1"
                     /translation="MLTLTELLQNSHWATGLSRDELARASAGTTERAVAEGGYVCHKG
                     EPVEHWIGIVDGLVKISNFSFEGKAITFTGVPPGGWFGEGSLMKSELRKYDVVALQDS
                     RVAYMNRGTFHWLLDHSISFNRFLIAQLNERLAQAYARIESDCLLGPDGRVARCLANL
                     FNPVLYPGLGRQLRVSQEELGYLTGLSRQRVNRALAALAEQNLLRARYGRIEVLDLDG
                     LRGYSG"
     gene            7623..9584
                     /locus_tag="VIS73_10615"
     CDS             7623..9584
                     /locus_tag="VIS73_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5763646.1"
                     /translation="MPDGKHRQALDTFPRLLFFHAQRRAEKTAIREKEYGIWQTWSWA
                     EAGDNVRALACGLAELGFSRGDNLAIIGDNRPRLYWSMAAAQCLGGVPVPLYQDGIAA
                     EVKFVLENAEVRFAIVEDQEQVDKLLEIRDQLPNLSHIIYDDPRGMRHYNQPFLHGIE
                     EVLEMGRIHARNHPDFLDDEVAKGKGDDVAIMLYTSGTTGNPKGVCQTHGSLIAAAEG
                     GCQFDNLTADEEILSYLPMAWVGDNLFSYAQALVAGFTINCPESGDTVMTDLREIGPT
                     YYFAPPRVYENLLTTVMIRMEDAGFIKRRMFHYFMDVARRCGAEILDGKDVSASDRLL
                     YALGNLLVYGPLRNVLGMSRIRVAYTAGAAIGPDLFRFYRSIAINLKQLYGSTETCAY
                     VCLQPDGGVKLDSVGKPAPGVEVKIADNGEVTVRGKAMLSGYYKRPDATAEAFNAEGY
                     FLTGDAGYFDDEGHLKIIDRAKDVGRVANGTMFAPNYIENKLKFFTHIKEAVAFGDGR
                     DYVTAFINVDPAAVGDWLERRGIAYSGYPDMAAKDVVYDLVKECVEQVNADLAQDPTL
                     GGSQVKRFLVLPKELDADDGELTRTRKVRRGFIAERYGDLIEALYSGQESCHITTVVK
                     YEDGRSNSIEADVRICDANMLPWSPAKAA"
     gene            9602..>10149
                     /locus_tag="VIS73_10620"
     CDS             9602..>10149
                     /locus_tag="VIS73_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5763647.1"
                     /translation="MAKTIGDVVLDLQNISLAFGGVKALTDISFDVREHEIRAIIGPN
                     GAGKSSMLNVINGVYTPQQGRILFDGRERKQMEPHDAAVHGIARTFQNIALFKGMTVL
                     DNIMTGRVTKMKTNFLQQAIWFGPARREELAHRRKVEEVIDFLEIQHIRKTPVGRLPY
                     GLQKRVELGRALAAEPSMLLLD"
BASE COUNT         1692 a         3278 c         3414 g         1765 t
ORIGIN      
        1 tcggcgttgc gcttcttgat cgctgcgaag agcacgcgct cttcttccga ggctggcttg
       61 ccgagaaccg aggggtcctt ttcggcgcgg gcgcccagat gcagcagcag cgttgcgtcg
      121 cctccgtcgt cgaggatcat gtttggcgtt ccgccatcgg cccattcgaa gatacggtgc
      181 gtgtagtccc agtactcggt cagggtctcg cccttgactg cgaacaccgg cgtgccggcc
      241 gcggcgatcg ccgcggcagc gtggtcctgc gtcgagaaga tgttgcacga tgcccagcgg
      301 acgtctgcgc cgagtgcctg cagcgtctcg atcagcacgg cggtctggat cgtcatgtgc
      361 agcgagccgg cgatgcgcgc gcccttgagc ggctgggcct tggcgaattc ctcgcggatg
      421 gccatcaaac cgggcatctc ggtctcggcg atacggattt ccttgcggcc ccagtcggcc
      481 agcttcaggt cggcgacaac gtgattcttg atatcagcca cagtgttcat agcgaactcc
      541 tacgatgtgg ccgggggggg tggcgagggc ggttctgggc tcgacatccc caacccggca
      601 gggttggtcg gtcgagcgcc gttgaagcag accggagcgt tccgggggtc gagcctggca
      661 gaccatgctg tcgcagcgct cctcgaccga ggcgcggatt atagacgaaa cgtcccccgg
      721 ttcagggcgg cgcgaccgct gagtgtcgga gccatccttg atcaagcacc gccggtcaat
      781 gcgctatttt tccagtcagg tcgagccaga accgatgttt catctaggaa ccatcgacta
      841 ccgggctgaa cggggcgaca cgacgcctgg caagggtgat tcgtttgagc attactgctc
      901 attatgtggg cgttgggcag acgatggtcg ccagtcggca ggcatcctgt cagggggaat
      961 ggtgagcgcg actgtgcgaa aatcagcggc gaatcacgag cgtggcgccg atcgcggcgg
     1021 gattcgaggg aggacaggga tggcgaaggt caaactcggg gggcgactct ggttgcgtgc
     1081 ggcgctgtcg tgcctgctgt cggtcgtcgc tgtgcccgtc ctggcggccg accaagccga
     1141 gcgcgcgctg gcgttcgttg ccgaactggt tgcgcgcggc gagattcgcg ccggcacgac
     1201 gcttaggctg gcgtttaagc ccggcaatat caacgcgctg ctcggcgccg acctcgaact
     1261 gcagcgcgag tgggaggaaa agaccggcac gcggatcagc gcccgggtca tgcccaacca
     1321 gccgacgctg gccaacttcg ccagctatcc cgattttgat ctgaccgtcg gccggcccta
     1381 cgagcaccag gagttgctcg acaaggcgct gattgcgccg ctcgacgatc tggctgcacg
     1441 cttcggattt gccattgacg accacggcgc cagcggattc atccacccgc gactgcaggc
     1501 caccttcggc ggtcggctgc ttgccattcc cgccgacggc gacgtgacgc tgctctacct
     1561 gcggcacgac ctgctcgacg atcccgccga acagaaggcc tatcgcgcgc gcactggccg
     1621 cagcctcgca atccccagga cctgggccga atacgacgcc caggtacgtt tcttccaccg
     1681 ccccgacaag gagttctacg gcgccgccga ggagcgtgac ccggagggtg ggtggatgta
     1741 ctggatgcag cgctatctgc cgcaacaggc gccgtaccag ccgctgttcg acgagcaggc
     1801 gcggccgctg atcgattcgc cggccggcgt cagcgcgacc gagcattatg tccactcggc
     1861 acgccacggt ccgccgcagg ccaccgaaga ggggcgcgac tacagctacg tgctaccgct
     1921 gttcatgcag ggcaaggctt attcggcgat gaccacgatt gccggcgcca agctgtttac
     1981 ctcggcggga tcgtcggtac tcggccgttt ttcggcgatt ccgatgcctg gggtcatggt
     2041 cgacggccgg ttggtccggc gcaacatgcc catctacggc aacaacctga tggtcgccaa
     2101 ggcatctccg aaccaggcgc tggccttcct gttcgcgatg tggctgaccg atcccgtcgt
     2161 ttcgctgcga acggttggcg cccgcggtgg ccataccgac ccctaccgtt ggcatcacct
     2221 gaccgacccg cgaatcgaat cgatctacac gccggcggcg ctgtcggtat ttgcccgtga
     2281 atgggacgtc gcggtgcctt cggggatggg gctgatcgac gaaagcgaat acgcgaccgt
     2341 tctcgatcgc cagctctggc tcgccgcgcg cggcgagatc acgcccgccg aggcgatgaa
     2401 gcgcacagcg cgcgagtggg agcggctgac cgagcgcatc ggccgcgacc ggcagatcgc
     2461 ctcgctgcag gccttccgcg ccgaaatgct gcgccgcgaa ccgttgccgc ccggcggcgg
     2521 acgatgattc ggtcgctgtt gtgaatacgc gccgccgttt cgggctggtc gcccagtcgc
     2581 tattcggttt cgccgtcggc ggcgtcctcg tcgcgatact gatctttgcc ggcgaccgca
     2641 tgttgcgcac gggcatcgcc cggctgcagg cgacgctcga ccagcagatc ctgccgttgg
     2701 ccgatctgca gcgcctgcaa tcacgcctca actacctgcg tctcgacgag ctcgagatgc
     2761 tgtcggtgcg cgatgtcttc cagttgccga cccaggccgc acgcatgcgc ggcgacttgc
     2821 gcgtcgtcga cgcggcgctc gccgagttcg cggagcggct gggacaaaac cagccggccg
     2881 actcgatccg cctgcgggga cactggcagg agtatcgcgg tctgctcgac ggcaagctgc
     2941 gtcatgccga agcgatggac attgcggcga tccagcgtga atcgaaaaca acgtcctatc
     3001 tgccttacgt cgcgatcgag agcttgcttg ccgagatcgc cgtcgccaag cgcacggcgg
     3061 ccgagcaggc ctaccagcgc acggtcgacg aacaggccgg ccagcgccga gtgttcatga
     3121 gcttggtgct cgcgggcacg ctgctgctga tcacggtgct ggcctggtcc gggcggactg
     3181 tcattcgccg gatcggcatc ctcaacgaca gcgcgcgccg gctggcgtcg ggtggcgagg
     3241 gctggcacgc cgatgtcggt ggcaatgacg agatctccga tctggccgag gccttcaacc
     3301 gcatgcgcga agaggtgctg gcccgcgagc gggcgctgca ggaagcacgc gacgaactcg
     3361 agaaccgcgt cgtcgagcgc acgcgcgaac tgcgcgattc caacgagcgg ctgacgctgc
     3421 tctccgaagt cgtcgagcag aaccccgttg gtatcctgat cgctgatcgg tcgggccgca
     3481 tcgactatgt caatccggcg tattcgacgc tgaccgggca gccggcgtca cgcctggtcg
     3541 gtcagccgct cgagctgctg ctcgcggtcg atcacatgcg tgccgccgcc gaggcgacgc
     3601 cggccgttct cgacggcggc cccgagtggc aggacgagca ggtcagctac cgcgacgacg
     3661 gcggcgagta ctgggagcgc ctgcgcatcc tcgccgtgcg cgacgccaac ggcaacagct
     3721 cgcacctgct gatgatccgc gaggacatca gcgagcgccg cgagcaggag gagcggattg
     3781 cctaccaggc gcactacgac gcgctgaccg cgttgcccaa tcgcgtgctg gcgctcgacc
     3841 gcctggtgca ggtgaccggg caggcccgtc gtgacggcga gcgcgccggc gtaatgttcc
     3901 tcgatctcga caacttcaag gaaatcaacg acacgcttgg gcacgaagcc ggcgacgagt
     3961 tgctccagct ggcggcgacg cgcctgcgcg gtgcgatgcg cgaggccgat accgttgccc
     4021 ggctcggcgg cgacgagttt ctggtgatcc tcgccggtct tggccgtggc aaggatgcgg
     4081 cgggcgtcgc cgacaagatc atcgagtcgt ttacggcacc gtttccggtt gccgggcgcg
     4141 acctagtggt ctcgccgtcg atcggcctgt cgatcttccc cgatgacggg gacgagcccg
     4201 ccgtcctgct gcgcaatgcc gatctggcga tgtacgaggc caaggaggcc gggcgcaaca
     4261 cctatcgttt cttcaatcag gcgatccacg acctgtcgct gcgccgtctc gagatcggcc
     4321 gccgccttcg cttggcgatg gccggcggcg agctgcgcct gctctaccag ccgctgatcc
     4381 acgccccgag cggccggatg atcggcgccg aagccttgct gcgctgggac aacgatgaac
     4441 ttggatcggt ggcgccggag atcttcattc cggtcgccga gcagggcggc ctgatagtcg
     4501 agatcggcgg ctgggtgctg cgcgaggcct gcgcgcgggc agcgcactgg cgcacccggc
     4561 agcccgattt cctgatggcg gtcaacgtct caccgcgcca gttccgcgaa accgattttg
     4621 ccgaactcgt cggcaggacg ctcgccgagc agaagctgcc ggccgccgcg ctcgagatcg
     4681 aggtcaccga aggcctgttg ctgcacaacc aggacgacgt caaggccgtg ctctctgcgc
     4741 tgcgtgagct tggcgtgcgc ctgtcgatgg acgacttcgg taccggctat tcctcgctaa
     4801 gctacctgcg cgccttcccg ttcaccacgc tgaaaatcga ccgcggattc gtccgtgacg
     4861 tgtctgagga tgctggtgac cgcgatctgg ttatcgccgc cgtcggcatg gccaaggcgc
     4921 ttggcctgcg ggtcatcgcc gaaggcgtcg agaccgacgc gcagtggatg ttcctcaacg
     4981 gcatcgactg cgacatcgtc cagggatacc gtttcgggaa gcccgtcagc gcgcaggatt
     5041 tcgaagcgac ctggctgtcc ggcattcgcg agcactgact gcggcgcaga cgggatgtga
     5101 cccgcctgcc gctttcaggc cagcgcgtca gggcgtgcgg cgacgcgcca gcaacgccgg
     5161 cgcaccactg gccgggcgct gcgccgcggg gcgtgctgcc ggcttggcag ccgggctgcc
     5221 gcccgccttc ggcttgcccg atgccgcgcc accctgcggc cgccgctgcg aattgccgcg
     5281 gcccggtgat tgaccacggc cgggaggacg cggcgggcgg ctgtcggcct cgaccatggc
     5341 cggcggcggc gggccgtcgt agcccggcat caccacctga tgcaggcgtt tcttcagcag
     5401 ccgctcgatg ccacgcagga aatccatctc gtcgggcgat accagcgaca cggcgtggcc
     5461 tggactgccg gctcgaccgg tgcggccgat gcggtgcacg tagtcttcgg agacgttcgg
     5521 cagctcgtag ttgaccacgt gcggcagctg ttcgatgtcg aggccgcgcg ccgcgatgtc
     5581 ggtggcaacc agtactcgga tgtcgccgtt cttaaatccc gagagtgcct tggtccgggc
     5641 gccctggctc ttgttgccgt gaatcgctgc ggcggttatg ccatccttgt tgagttgctc
     5701 ggcgaggcgg ttggcgccgt gtttcatgcg cgtgaatacc agcacctgct cccagcggcc
     5761 gtcggcgatc aggtcgcgca gcagttcgcg cttgcggttc ttggcgaccg ggtgaatgac
     5821 ctggctgacc agttcgctgt cggcgttgcg gcgcgccact tcgacactgg ccgggttgtc
     5881 gagcaggcct tcggcgaggc ggcggatttc gtcggagaag gtcgccgaga acagcagcgt
     5941 ctgctttttc gccggcagca gcttcaacac ccgcttgatg tcgtggatga agcccatgtc
     6001 gagcatgcgg tcagcctcgt cgaggacgag aatctcgatc gccgagaggt ccacggtgcg
     6061 ctggccggcg tggtcgagta ggcgaccggg ggtggcgacc agcacgtcga cgccgggctt
     6121 cagcgcggca atctgcgggt tgatgccgac gccgccatag accagcgtcg acttgagcga
     6181 caggtgctgc ccgtaggtct tgatcgcttc ctcgacctgc gcggcgagtt cgcgggtcgg
     6241 cgtcagtacc agcgcgcgga cgcgccttgc ggccggctta agcgggttcg acgcgaggcg
     6301 ctgcagcagc ggcagggcga agccggcagt cttgcctgtg ccggtctgcg ctgccgcgag
     6361 tacgtcgcgg ccggcaagga tcaacgggat cgcctgagtc tggatggggg tgggcgtggt
     6421 gtagccttcg tcggcaacgg cacgggcgag ctcggccatc aggccgagcg attcaaattc
     6481 agatttcaag atgactcctg cacgcgtccg cgcgctctga tgcgcgttcc ggtacggggc
     6541 gtggcaacgg atgggtaggg gcgcgggttc cgctgcgcga caggcgaggc tggctcgctg
     6601 tggcggtgtc cggtcggggc ggcgccgggg gcgctggcaa cgacgcggaa ggaacctgcg
     6661 gacgcccggc ttgaccggat tgcacaggtc gggcgaaggc gcattgtacg ccttatggcg
     6721 cggcgcagca gatttgcgcg gtgagcggct gggatcagcc gctgtagccg cgcaggccat
     6781 cgaggtcgag cacctcgatc cgaccatagc gcgcgcgcag cagattctgc tcggccagcg
     6841 ccgccagggc gcggttgacg cgctggcgcg aaagccccgt gaggtagccg agttcttctt
     6901 gcgagacccg caactggcgg ccgagccccg ggtagagcac cgggttgaag aggttggcga
     6961 ggcagcgcgc gacgcggccg tcgggtccca gcaggcagtc cgattcaatc ctcgcgtagg
     7021 cttgcgccag ccgctcgttg agctgggcga ttaggaagcg attgaagctg atgctgtggt
     7081 cgagcagcca gtggaaagtg ccgcgattca tgtaggcgac acggctgtcc tgcagcgcga
     7141 ccacgtcgta cttgcgcagc tcgctcttca tcagcgatcc ctctccgaac cagccgccgg
     7201 gcggcacgcc ggtgaaggtg atcgccttgc cctcgaagga gaaattgctg atcttgacca
     7261 gaccgtcgac gatgccgatc cagtgttcga ccggctcgcc cttgtggcag acgtaaccgc
     7321 cttcggcgac cgcgcgctcg gtggtcccgg cgctggcgcg ggcgagttcg tcgcggctca
     7381 gtccggttgc ccagtgcgag ttctgcagca gttcggtcag cgtcagcatg gtgttccgtg
     7441 gcgaggtgac agcaaattgt ccatgaattg tctgttcggc gacaattgaa ttcgacatgc
     7501 ttggctacac tgccagcaga caaaagctgt gcgccgcatt caaacgaccg gaaaaccggc
     7561 agcgcacgcg acgataacgg gttggagaca cgtgccttca tcggcgcgcg aggaggggat
     7621 tcatgccgga cgggaagcat cgtcaggcgc tcgacacctt tccgcgactg ctgttcttcc
     7681 acgcgcaacg acgcgcggaa aagacggcga ttcgcgagaa ggagtacggc atctggcaga
     7741 cctggtcttg ggccgaggcg ggcgacaacg ttcgcgcgtt ggcatgcggc ctcgctgaac
     7801 tcggtttttc gcgaggcgac aatctcgcca tcattggcga caaccggccg cgcctctact
     7861 ggtcgatggc cgctgcgcag tgcctgggcg gtgtcccggt gccgctctat caggatggca
     7921 tcgccgccga ggtgaagttt gtcctcgaga acgccgaggt tcgcttcgcc atcgtcgaag
     7981 atcaggagca ggtcgacaag ctgctcgaga ttcgcgacca gctgcccaat ctcagccaca
     8041 tcatctacga cgatccgcgc ggcatgcgcc actacaacca gcccttcctg cacggcatcg
     8101 aggaagtgct ggagatgggc cgaatccacg cccggaacca tcccgatttt cttgacgacg
     8161 aggtcgccaa ggggaagggc gacgacgtcg cgatcatgct ctacacctcg ggcaccaccg
     8221 gcaatccgaa aggcgtctgc cagacccacg gctcattgat agccgccgcc gaggggggct
     8281 gccagttcga caatctgacc gccgacgagg agatcctctc ctacctgccg atggcctggg
     8341 tcggagacaa cctgttctcc tacgcgcagg cgctggtcgc cggcttcacg attaactgtc
     8401 ccgagtcagg cgacacggtg atgaccgacc tgcgagagat cggcccgacc tactacttcg
     8461 cgccgcctcg ggtttatgaa aacctgctga cgacggtgat gatccgtatg gaagacgccg
     8521 gcttcatcaa gcgccggatg tttcattact tcatggacgt cgcgcgccgt tgcggcgccg
     8581 agatcctcga cggcaaggat gtctcggcca gcgaccggct gctctacgcg ctcggcaacc
     8641 tgctggtcta cgggccgctg cgcaacgtgc tggggatgag ccgtatccgg gtggcctaca
     8701 ccgctggcgc ggcgatcggc cccgacctgt ttcgctttta ccgctcgatt gcgatcaacc
     8761 tcaaacagct ctacgggtcg accgagacct gcgcctacgt ctgcctgcag cccgatggcg
     8821 gcgtcaagct cgattcggtc ggcaagccgg cgcccggcgt cgaggtgaag attgccgaca
     8881 acggggaagt cacggtccgc ggcaaggcga tgctctcggg ttactacaag cgtcccgacg
     8941 cgacggccga ggccttcaac gccgagggct atttcctcac cggcgacgcc ggctatttcg
     9001 acgacgaggg gcacctgaag atcatcgacc gcgccaaaga cgtcggcagg gttgccaacg
     9061 gcacgatgtt cgcgcccaac tacatcgaga acaagctcaa gttcttcacg cacatcaagg
     9121 aagccgtggc cttcggcgac ggccgagact acgtcaccgc cttcatcaac gtcgacccgg
     9181 ccgccgtcgg cgactggctc gaacggcgcg gcatcgccta ctcgggttat cccgacatgg
     9241 cggccaagga cgtcgtctac gacctggtca aggaatgcgt cgagcaggtc aatgccgacc
     9301 tggcacagga tccgacgctg ggcggctcgc aggtcaagcg ctttctggtg ttgcccaagg
     9361 agctcgatgc tgatgacggc gagttgacgc gcacgcgcaa ggtacggcgc ggcttcatcg
     9421 ccgagcgcta cggcgacctg atcgaggccc tctattcggg gcaggagtcc tgccacatca
     9481 ctaccgtcgt caagtacgag gacgggcgca gcaacagcat cgaggccgac gtacgcatct
     9541 gcgatgcaaa catgctgccc tggtcaccgg cgaaagccgc ctgagcgcgg aagggagaaa
     9601 catggccaag accatcggcg acgtcgttct cgatctgcaa aacatctcgc tcgcgttcgg
     9661 cggcgtcaag gcgctcaccg atatttcctt cgacgtccgt gagcatgaga tccgcgccat
     9721 tatcggcccc aacggcgccg gcaagagctc catgcttaac gtcatcaacg gcgtctacac
     9781 gccgcagcag ggacgcatcc tgttcgacgg tcgcgagcgc aagcagatgg agccgcacga
     9841 cgcggccgtg cacgggatcg cgcgcacctt ccagaacatt gcgctgttca agggaatgac
     9901 cgtgctcgac aacatcatga ccgggcgcgt taccaagatg aagaccaact tcctgcagca
     9961 ggcgatctgg ttcggaccgg cgcggcgcga agaattggcc caccggcgca aggtcgagga
    10021 ggtgatcgac ttcctcgaga ttcagcacat tcgcaagacc ccggtcgggc gcctgcccta
    10081 cggcctgcag aagcgcgtcg agctcggccg cgcgctggcc gccgaaccga gcatgctgct
    10141 gctcgacga
//
LOCUS       DATDAA010000352         2836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_306147,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000352 DATDAA010000000
VERSION     DATDAA010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306147"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..1271)
                     /locus_tag="VIS73_10625"
     CDS             complement(312..1271)
                     /locus_tag="VIS73_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5763648.1"
                     /translation="MGFGVAFALAAGLMWGLVFVGPLLLPEYPAALQSFGRYLAFGLI
                     ALPLGWLDRREIARLTSSDWIEALKLAAIGNVLYYLCLASAVQRAGGPLPTMIIGTLP
                     VVIAITANLRDHRRDGSLPWARLLPSLLLIAAGIACVNHDELTVIAGQSADERARYFL
                     GAVFAVGALACWTWYPLRNADWLRNHPGRNPRAWATAQGLATLPLALLGYVGLWGWLA
                     ATGSDFAMPVGPRPLVFIGLMLAIGFFASWLGTLCWNEASQRLPTALVGQLIVFETLA
                     ALAYAFALRGEWPGEFTATGIGLLIVGVGWALRIKPVHTSAIV"
     gene            complement(1368..2075)
                     /locus_tag="VIS73_10630"
     CDS             complement(1368..2075)
                     /locus_tag="VIS73_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5763649.1"
                     /translation="MSDEVIKIVQVDDHGLCRQALSDLLEARGGMKVLATIGDPLEAP
                     EILKKLKPDLAIIDLRMGALDGLSLVRRLGEEGSDVPIVILTMSDSKEDMATALRLGV
                     RGYLLKDMEPADVIDSIRRAAKGELVVAPAMSNKLASVLQDGPTKEKKTSLQQLTDRE
                     RQILEHVAAGKSNKAIALSLNISHDTVKLHVRHILAKLNMSSRVEAAVFAVEQQMTGQ
                     MSEGEPITRLPVRNRSH"
     gene            complement(2068..>2836)
                     /locus_tag="VIS73_10635"
     CDS             complement(2068..>2836)
                     /locus_tag="VIS73_10635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5763650.1"
                     /translation="EDCRPNIKTLLRPIGQLLGLTLENSILERERSRASIVHERQAMA
                     ADIHDSLAQTLAFGRMRVPLIEDAVVEHDTQQALKFCDELKRELTTAHGALRGLITQF
                     RAGLDSAGLRRGLREAIARFREASGIALEFDNQIPDLKLRGEVEVQVFYIVQEALANV
                     CKHAKATRTRLSIEYVEGGVEFSVEDNGAGQGVGQIAQRSGKEGQGSFGLRIMQERAE
                     RFGGRLDIENMPKGGTRVRVFIPKDAVTEGTGEIRNE"
BASE COUNT          522 a          922 c          916 g          476 t
ORIGIN      
        1 tgctccgctc ggagaccagc cgtaggatca ccggaacggc gaatgtcgcc tcggggtcgg
       61 gtccggtcct accgaccctg cggcagatca cccgaaagca gtcgctgaaa actaagggca
      121 gcaacctggc gggcagcagt cgcttaattc cactggtgcg accggcagca aaggccgaaa
      181 acgcgccact tctaacctac cgaagcgcgt taagggcttg ggagagccct cgacggaatc
      241 gaccaacggt gcatgagcga agcctctcgc ccactgcgcg gatcactcgc gcgccattgc
      301 ggaacgcatg gtcagacgat cgccgaggta tgcacgggct tgatgcgcag cgcccagccg
      361 acgccgacga tcaggaggcc gataccggtc gcggtgaatt cgccaggcca ttcgccgcgc
      421 agcgcgaagg cataggccag cgcggccagc gtttcgaaga cgatgagttg gccgaccagc
      481 gcggtcggca ggcgctggct cgcttcgttc cagcacagcg tgcccaacca tgaggcgaag
      541 aatccgatcg ccagcatcag gccgatgaag accagtggcc gcggcccgac gggcatcgca
      601 aaatcgctgc cggtggcggc cagccatccc cacagcccga cgtagccgag cagcgccagc
      661 ggcaaggtag ccaacccctg tgcagtagcc caggcgcgcg ggttgcggcc cggatggttg
      721 cgcagccagt cggcattgcg cagcggatac caagtccagc aggcaagcgc cccgactgcg
      781 aacaccgcgc caaggaaata gcgagcccgt tcgtcggccg attggccggc gatgactgtg
      841 agttcgtcgt ggttgacgca tgcgatgcct gccgcgatga gcagcagcga gggcagcagg
      901 cgcgcccagg gcaggctgcc gtcgcgtcgg tgatcacgca ggttggcggt gatcgcgatg
      961 accactggca gggtgccgat gatcatcgtc ggcagggggc cgccggcgcg ctggacggcg
     1021 ctggccagac acaggtagta aaggacgttg ccgatagcgg cgagcttgag cgcctcgatc
     1081 cagtcgctgc tggtaaggcg cgcgatctcg cggcgatcga gccagccgag tggcagggca
     1141 atgagaccga atgccagata acgcccgaag gattgcaggg ccgcggggta ctcgggcagc
     1201 agcagcggac cgacgaatac caatccccac atgagccctg cggcaagcgc gaaagcgaca
     1261 ccgaagccca cgagctgaaa cctccgaaaa cggccgtcag gccggtcgaa tcacatcagg
     1321 cgttgcggcg gtagctcaaa cgcgcgcccg ctgactggct ttgctaccta atggctgcgg
     1381 ttgcgcaccg gcaggcgggt gatcggctcg ccctcgctca tctgtccggt catctgctgc
     1441 tcgacggcga agacggccgc ctcgacgcgc gaggacatgt tgagcttggc gaggatatga
     1501 cgaacgtgca gcttgacggt gtcgtggctg atgttgagcg agagcgcgat cgccttgttg
     1561 cttttgccgg cggcgacgtg ttcgagaatc tgccgttcgc ggtcggtgag ctgctgcagc
     1621 gaggtcttct tctcctttgt cggaccgtcc tgcagcacgc tggccagctt gttcgacatc
     1681 gccggcgcaa ccaccagctc gcccttggcg gcacggcgaa tggagtcaat gacgtcggcc
     1741 ggctccatat ccttaagcag atatccgcga acgccgagcc gcagcgctgt cgccatgtcc
     1801 tccttcgagt cgctcatggt caggatgacg atggggacgt cactgccctc ctcgcccaga
     1861 cggcggacca gactcagccc gtcgagcgcg cccatgcgca ggtcgatgat cgccagatcg
     1921 ggcttgagct tcttcaggat ctccggtgct tcgagcggat cgccgatggt cgccagcacc
     1981 ttcatgcctc cgcgcgcctc gagcaggtcg gacagcgcct gacggcaaag gccatggtcg
     2041 tcgacctgaa cgatcttgat cacttcgtca ctcattgcga atctctcccg taccttcggt
     2101 gactgcgtct ttaggaatga acacgcggac ccgcgtgccc cccttgggca tgttctcgat
     2161 atcgagccgg ccgccgaagc gctcggcgcg ctcctgcatg atacgcagac caaacgatcc
     2221 ttggccttcc ttgccactcc gctgcgcaat ctgcccgacg ccttgaccag cgccgttgtc
     2281 ctcgaccgaa aactccacgc caccttcaac gtactcgatg ctgagccgcg tgcgggtcgc
     2341 cttggcgtgc ttacagacgt tggccagtgc ttcctggacg atatagaaaa cctgcacctc
     2401 gacctcgccg cgcaatttga ggtcgggaat ctggttgtcg aactcgagcg cgatgccact
     2461 cgcctcgcga aagcgcgcga tcgcctcgcg cagaccacgc cgcaggccgg ctgagtctag
     2521 gccggcacga aactgcgtga tcagcccgcg cagcgcgccg tgcgcggtgg tcagttcgcg
     2581 tttaagttcg tcgcagaact tcagggcctg ctgcgtatcg tgctcgacca cggcgtcctc
     2641 gatcagtggg acgcgcatgc ggccaaaggc cagcgtttga gccagcgagt catggatgtc
     2701 ggcggccatc gcctgccgtt cgtggacgat gctcgcgcgc gaacgctcgc gctcaagaat
     2761 cgagttctcc agcgtcaggc cgagcagctg gccgatcggt cgcagcagcg tcttgatgtt
     2821 gggacgacag tcctcg
//
LOCUS       DATDAA010000353         4506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_515217,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000353 DATDAA010000000
VERSION     DATDAA010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515217"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VIS73_10640"
     CDS             <1..647
                     /locus_tag="VIS73_10640"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY5763651.1"
                     /translation="GDNRKVNVLAYVQASVKPIQILYHSVDSVLPQICAEKKSAVFQD
                     LYKSFTSRRKPKCLTIERINLQSLSDAEHAALDKHSLSFSSGPMIRLTMATSDGRYGT
                     LALAFTSTISADEEEAYIESLKKWGHQYVTSQLEDSLSRSLWEAPFKDPYPITVTGAN
                     PTSTAPVTQPAGAQPAPSSDDVFSMLEKLKSLHEQGILTDQEFNSKKAELLKRL"
     gene            complement(702..953)
                     /locus_tag="VIS73_10645"
     CDS             complement(702..953)
                     /locus_tag="VIS73_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763652.1"
                     /translation="MASWLARLGAPRGRAATCATAYALVATLPLLGIVNTPRTLYLVS
                     EAAQYAVGNVAEWYGGPIFTAFFLLRAWLWRQTSFAANP"
     gene            complement(1122..1835)
                     /locus_tag="VIS73_10650"
     CDS             complement(1122..1835)
                     /locus_tag="VIS73_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763653.1"
                     /translation="MSPEPKRGTIALWGLPLLAGLLPLLATVIAFNLSVAQGYFATCN
                     PFIDGCVSISRAAREGLGNILFRALLLPAATLQAIVWLLSGSWLRQLGAPHDRLLRVL
                     PWIGVTAAVFLMLYGTFLGTEGEGYRLMRRYGINVYFGFTCIAMLIVAGALRTASRVR
                     RELSHSALLVYALVAALPLLGIINTMRTLYLNSEAAQYAVGNITEWWGGLMFTVFFVV
                     VAWLWWRTRFDAALRASIV"
     gene            complement(1857..2579)
                     /locus_tag="VIS73_10655"
     CDS             complement(1857..2579)
                     /locus_tag="VIS73_10655"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY5763654.1"
                     /translation="MRRTSPATIATGVVRVALAFASALLAAGVALAAPDRVEIRYAVS
                     YGEARVFEESDLFVRDGDRYSIVSEAKPVGIAALFLSDIRRESRGRVTRGGLQPERFV
                     DDRGRRGQRIAEFDWAAQRITLRHDGVVETRELAVGTLDQSAFHFNFMFAPPVEAGLA
                     VTLSDGRRVKDYRYRFVGREKLTTSVGEIDTLHFAREIAAGDARTFDVWLAPAHHHLP
                     VRIRYVEGNDVFDSRVTGIEIR"
     gene            complement(2566..>4506)
                     /gene="polA"
                     /locus_tag="VIS73_10660"
     CDS             complement(2566..>4506)
                     /gene="polA"
                     /locus_tag="VIS73_10660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY5763655.1"
                     /translation="QGFRSWLKALNDPEGAKGEAKPAAAAADAPTPAALDASGKHREA
                     YATVLDEGAFANWMQRIEAADAVAFDSETTSLDPLAAEIVGLSFALGDGDACYIPLAH
                     HYAGVPDQLDRDTVLRRLKPWLENPGAKKIGQNLKYDQHVLANHGIALRGVADDTLLE
                     SYALDAGREGVRGHDLGELARRHLGLETIAYTELCGKGASQIGFEQVDIERAAAYAAE
                     DADLTWRIHRVLSERLAAEPAIGTVYRDIEMPVREVLFRMERNGVLIDGERLSRQSHE
                     IGQRLMALEGEAHALAGQPFNLASPKQLGEILFTRLGLPVKKKTPSGGPSTDEEVLTE
                     LALDYPLPKLLLEHRSLAKLKGTYTDKLPLMVNAGTGRVHTSFSQSTAVTGRLASSDP
                     NLQNIPVRTAEGRKIRAAFVAPQGAKIVSADYSQIELRIMAHLSGDSRLCEAFAAGED
                     IHRATAAGIFGTDLDAVGPDQRRVAKVINFGLIYGMSAFGLARELGLERGAAQAYIEQ
                     YFARYPGVARYMEATRASAHDRGYVETVFGRRLWLPEIRARQQARRQGAERAAINAPM
                     QGTAADLIKLAMIAVQDWLTAGKLATKLVLQVHDELVLEVPNAELARVQEHLPKLMTG
                     VAELKVPLAVDVGTGDNWDEAH"
BASE COUNT          858 a         1421 c         1449 g          778 t
ORIGIN      
        1 taggagacaa ccggaaagtg aacgtgctgg cctatgttca ggcatcggtt aaacctatac
       61 aaattctgta tcactcagtt gattcagtcc ttccgcaaat ctgcgccgaa aagaaatctg
      121 cggtattcca agacctttac aagtccttta ccagccgccg taaaccgaag tgcctaacga
      181 ttgaaagaat taacctacag tcgctttctg atgctgagca cgctgcactc gacaagcact
      241 cgctcagctt ttcctcggga ccaatgattc gattgaccat ggcaacttcc gatggtcgct
      301 acggcaccct ggctctggcc ttcaccagca cgatttcggc cgacgaggaa gaggcataca
      361 tcgagtcact taaaaaatgg gggcaccagt acgtcacttc acaactcgag gactcgttga
      421 gccgatcctt gtgggaagcc cccttcaaag acccgtaccc aatcacagta acgggagcca
      481 atccgacttc gaccgctccg gtcacccaac cggctggcgc acaacctgcg cccagttctg
      541 atgacgtgtt ttcgatgctc gagaaactca agtcgctcca cgagcagggc atcctcaccg
      601 accaagaatt caacagtaag aaagctgaat tactgaagcg cctgtaaggg actacttgtc
      661 cgacccgtag gcaattcgat ggactgtggc agaggctgcg cctatggatt agctgcaaag
      721 ctggtttgcc gccacaacca cgcccgcagc aagaagaagg cagtgaagat cggaccgcca
      781 taccactcgg cgacgttgcc aacggcgtat tgagctgcct cgctaaccag atagagcgtg
      841 cgtggggtat tgacgatgcc gagcagcggc agcgtcgcga ccagagcgta ggcggtggcg
      901 caggttgcag cgcggccgcg cggtgcgcct agcctcgcca gccatgaagc caaacgatcg
      961 cctgcagtgc cataaaggac agacaccgaa tgactccgtc cacacgacgt gactaattcg
     1021 taggaaagtc tgcttcggtt aatggcgaac agcggtttcg ggtcggcagc cgccggataa
     1081 cctttgcctg cggatgatcc gccctgccga tatacgtcag cttagacgat gctcgcccgc
     1141 agcgccgcat caaaccgtgt tcgccaccac agccaggcga cgactacgaa gaacacggta
     1201 aacatcagac caccccacca ctcggtgata ttgccgaccg catattgcgc cgcctcgcta
     1261 ttgaggtaca gcgtgcgcat cgtgttgatg atgccgagca gcggcagcgc ggcgactagc
     1321 gcgtagacga gcagcgccga gtggcttagc tctcggcgca cgcgagaagc agtccgcagc
     1381 gccccggcga cgatcagcat cgcaatgcag gtaaagccga agtagacgtt tatgccgtag
     1441 cggcgcatca ggcggtaacc ctcgccctcg gtgccgagga aggttccgta gagcatgagg
     1501 aagaccgcgg cggtgacgcc gatccacggc aagacgcgca gcaagcgatc gtgcggggcg
     1561 ccgagttgcc gcagccacga gccgctcaac agccagacga tcgcctgcag cgtcgccgcc
     1621 ggcagcagca gggcgcgaaa gaggatgttg ccgagccctt cgcgcgcggc acggctgatc
     1681 gagacgcaac cgtctataaa gggattgcag gtagcaaagt agccctgcgc gacggaaaga
     1741 ttgaaggcaa tcaccgttgc tagcagcggc agcagaccag ctagcagcgg caggccccag
     1801 agggcaatgg tgccgcgctt gggttccgga ctcatggcta ggcgcgccgg cggcagttag
     1861 cggatctcga tgccggtgac ccgcgaatcg aagacatcgt tgccttcgac gtagcggata
     1921 cgaaccggca gatgatggtg cgccggcgca agccagacgt cgaaagtccg cgcgtcgccc
     1981 gcggcgattt cacgcgcgaa atgcagcgtg tcgatttcac cgaccgacgt cgtgagcttc
     2041 tcccgaccga caaagcggta acggtagtcc ttgacccgcc ggccgtcact gagcgtgacc
     2101 gcaaggccgg cctcgaccgg tggcgcgaac atgaagttga aatggaaggc cgactgatca
     2161 agcgtgccaa cggccaactc gcgggtttcg accacgccgt cgtggcggag cgtgatccgt
     2221 tgggcagccc agtcgaactc ggcgatgcgc tggccacgcc ggccgcggtc gtcgacgaag
     2281 cgctcaggct ggagcccgcc cctggtgacg cggccgcggc tttcgcggcg gatgtcggag
     2341 aggaagagcg ctgcgatgcc gacgggtttc gcctcgctga cgatcgagta gcgatcaccg
     2401 tcgcggacga acaggtcgct ctcctcgaag acgcgtgctt cgccgtagga aacggcgtaa
     2461 cgaatctcga cgcgatcggg cgccgccagc gctacgccgg ccgccagcaa ggccgacgcg
     2521 aaagcgagcg ccacgcggac gacgccggtc gcgatggtcg ccgggctagt gcgcctcatc
     2581 ccagttgtcg ccggtaccga catcgacggc gagcggcacc ttgagttcgg cgacgccggt
     2641 catcagtttc ggcagatgct cctgcacgcg cgccaattcg gcattgggca cttcgagcac
     2701 aagttcatcg tggacctgca gaacgagttt cgtcgccagc ttcccggcgg tcagccaatc
     2761 ctgcacggca atcatcgcca gcttgatcag gtcggcggcc gtgccctgca tcggcgcgtt
     2821 gatcgccgca cgctcggcgc cctgccggcg tgcctgctgg cgcgcgcgga tttccggcag
     2881 ccacaggcgg cgaccgaaaa cagtctcgac gtagccgcgg tcgtgggcgc tggcgcgggt
     2941 cgcttccatg tagcgcgcga cacccggata gcgtgcgaaa tattgctcga tgtaggcctg
     3001 cgcggcgccg cgctcgagac cgagttcgcg cgccaggccg aaggcgctca tgccgtagat
     3061 caggccgaag ttgatgacct tggcgacgcg ccgctggtcg gggccgaccg catcgagatc
     3121 ggtgccgaag atgccggcgg cggtggctcg gtggatgtcc tcgccggcag cgaaagcctc
     3181 gcacagccgc gaatcgcccg ataggtgcgc catgatccgc agctcgatct gtgagtagtc
     3241 ggcggagacg atcttcgcgc cctggggggc gacgaaggcg gcgcggatct tccgcccttc
     3301 ggcggtgcgc accggaatgt tctgcaggtt ggggtcggaa ctcgccagcc ggccggtaac
     3361 cgccgtcgac tgcgaaaagc ttgtgtgtac gcgaccggtg ccggcgttga ccatcaaggg
     3421 cagcttgtcg gtgtaggtgc ccttaagctt ggcgagactg cgatgttcga gcagcagttt
     3481 tggcagcggg tagtcgagcg cgagttcggt cagcacctcc tcgtctgtcg acggcccgcc
     3541 cgagggcgtt ttcttcttca ccggaagccc gagcctggtg aagaggattt cgccgagctg
     3601 cttgggactg gcgaggttga acggctgccc ggcgagcgcg tgcgcctcgc cttcgagcgc
     3661 catcagccgc tgcccgatct catgactctg ccgcgacagc cgttcgccgt cgatcagcac
     3721 gccgttgcgc tccatgcgga acagcacttc gcgcaccggc atctcgatgt cgcgatagac
     3781 ggtgccgatc gccggctccg cggccagccg ttcactcagc acgcgatgaa ttcgccaggt
     3841 caggtcggcg tcctcggccg cgtaggcggc ggcgcgctcg atgtcgacct gctcgaagcc
     3901 gatttggctc gcgcccttgc cgcacagctc ggtgtaggcg atggtttcga gcccgaggtg
     3961 gcggcgcgcg agttcgccga gatcgtgccc gcgcacgcct tcgcggccgg cgtcgagcgc
     4021 gtaggattcg agcagcgtgt cgtcggcgac gccacgcaag gcgatgccgt ggttggcgag
     4081 cacgtgctgg tcgtacttga ggttctgccc gatcttcttt gcgcctggat tctcgagcca
     4141 gggcttgagc cgccgcagta ccgtgtcgcg atcgagctga tcgggtacgc cggcgtagtg
     4201 atgcgcgagc gggatgtaac aggcgtcgcc gtcgccgagc gcaaacgaca gcccgacgat
     4261 ctcggccgcc agtggatcga ggctggtcgt ctcgctatcg aaggcgacag cgtcggctgc
     4321 ctcgatgcgt tgcatccagt tggcgaaagc gccttcatcg agcacggttg cataggcttc
     4381 gcgatgcttt cccgaggcat cgagcgctgc cggcgtgggc gcatcagcgg cggcagccgc
     4441 cggcttcgct tcgcctttcg ccccctcggg atcgttcaag gccttcagcc agctgcgaaa
     4501 gccctg
//
LOCUS       DATDAA010000354         2876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_270225,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000354 DATDAA010000000
VERSION     DATDAA010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270225"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VIS73_10665"
     CDS             <1..270
                     /locus_tag="VIS73_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763656.1"
                     /translation="EGGLIERARELRQQGTVIEDTAPNRMLRMAPETSTLEEGLTEHY
                     CDETQTADPNSWLECILELERQGLHEAARLERERLVEAFPPPMLP"
     gene            402..1379
                     /locus_tag="VIS73_10670"
     CDS             402..1379
                     /locus_tag="VIS73_10670"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY5763657.1"
                     /translation="MKSPVRVTITGAAGQIGYQLAFRIASGQMLGNDQPVILQLLEIP
                     QALDALKGVVMELDDCAFRTLAGVVATDDPNVAFKDSDYGLLVGARPRGPGMERKDLL
                     EANAAIFSVQGKAINDHASRDIRILVVGNPANTNALITSSNAPDIDPANITAMTRLDH
                     NRAIAQLAQKTDSHVTDVKQMIIWGNHSATQYPDINHASVAGKPATELVDENWLSDEF
                     IPVVQQRGAAIIKARGASSAASAASAAIDHMHDWALGTNGDDWVSMAIPADGSYGIGP
                     GIIYSYPVRCSGGKYEIVQGLDINGFSRARMDATDAELREERAAVESLL"
     gene            1462..>2876
                     /locus_tag="VIS73_10675"
     CDS             1462..>2876
                     /locus_tag="VIS73_10675"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY5763658.1"
                     /translation="MSSFIVSLFWLLLFVGGAIYLAYQRIDLRTSTIAAGLAVLAYTI
                     FGEGAFLWKLVLWAAFGAMIVPNLIEFRREKITKPLLEVYRKMLPSMSDTEREALEAG
                     NVWWEGELFSGMPEWDRLMSYPAPKLSDEEQAFLDGPCETLCRMIDDWEICHERADMP
                     KEVWDYIIENRFFAMIIPKQYGGLEFSAYANACVIMKLASRSATASSTIGVPNSLGPA
                     ELLLHYGTEEQKNRYLPGLAAGTEIPCFALTSPQAGSDAASIIDSGVVCKGEWGGETI
                     VGIRLNWDKRYITLAPIATVLGLAFKLYDPDHLIGDQDEYGITAALIPTDTPGVTVGR
                     RHHPLTIGFQNGPTSGKDVFVPLDYIIGGRDMAGKGWKMLVELLSVGRAITLPSTAAG
                     GGQAASYATGAYAIIRKQFNTPIANFEGVGEALTRIAGYTYIMTSAVSVTSGAIDQGE
                     KPAVPSAILKYHCTELGRKVS"
BASE COUNT          569 a          940 c          891 g          476 t
ORIGIN      
        1 gaggggggcc tgatagagcg ggcccgggag ttgcgacagc agggaaccgt catcgaggac
       61 acggcgccga accggatgct tcgcatggcg ccggagacca gcacgctgga agaagggcta
      121 acggagcact attgcgacga aacacagacc gccgacccga acagctggct cgaatgcatc
      181 ctggaactcg aacgccaggg cctgcacgag gccgctcgtc tcgagaggga acgcctggtg
      241 gaagcctttc cgccaccaat gctcccctga tttcggttct tcccgatgtc ccgcgccggg
      301 cgcgatgcta gaatcgccgg ccccggttat tgcaaagtcg tgctgcgggc tcgccgggga
      361 acggcagcat ccattgagac cggccaacag ggaacaacac catgaaatca cccgttcgcg
      421 taacgatcac aggggctgcc ggccagatcg gctaccaact cgcctttcgt atagcctccg
      481 ggcagatgct tggcaacgac cagcccgtca ttctgcagct gctcgagata ccgcaggccc
      541 tcgacgccct caagggtgtc gtcatggaac tcgatgactg cgcattcagg acgctcgcgg
      601 gcgtcgtagc gacggacgac ccgaacgtgg ccttcaagga cagcgattac ggcctgcttg
      661 tcggcgctcg cccgcgcggc ccggggatgg aacgcaagga cctgctcgaa gccaatgctg
      721 cgatcttctc ggtccagggc aaggccatca acgaccatgc gagtcgcgac atccgcatcc
      781 tcgtcgtggg caacccggcc aacaccaatg cgctgatcac gagcagcaac gcgcccgaca
      841 tcgacccggc caacatcacg gccatgaccc ggctggacca caaccgcgcc atcgctcagc
      901 tcgcgcagaa gacggacagc cacgtcaccg acgtgaagca aatgattatc tggggcaacc
      961 actcggcgac ccagtacccg gacatcaacc acgcaagcgt cgcgggcaag ccggccacgg
     1021 agctcgtgga cgagaactgg ctgtcggacg agttcatccc ggtcgtccaa caacgtggcg
     1081 ctgccatcat caaggcccgc ggggcctcgt ccgccgcatc cgccgcctcg gccgccatcg
     1141 accacatgca cgactgggcg ctcggcacca acggcgacga ctgggtcagc atggcgatcc
     1201 ccgcggacgg cagttacggc atcggaccgg gcataattta ctcgtatccg gtgcgctgca
     1261 gcggcggcaa gtatgaaatc gtccagggcc tcgacatcaa cggcttcagc cgcgcgcgca
     1321 tggacgccac cgacgccgaa ctgcgcgaag agcgggctgc cgtcgagtcc ttgttgtgac
     1381 gtgacgccct cgatggcgca gcatgcgtca cgatgatagg gtttcaggga aacgcaattt
     1441 cctgattcgg gagtatcgcg cgtgtccagt tttatcgtga gccttttctg gctgctgctg
     1501 ttcgttggcg gcgccatcta ccttgcctat cagcggatcg atctgcgcac gtccacgatc
     1561 gcggccggac tcgccgtgct cgcgtacacg atcttcggtg agggcgcctt cctgtggaag
     1621 ctggtcctgt gggccgcatt cggcgccatg atcgtgccga acctgatcga gttccgccgc
     1681 gagaagatca ccaagccgct gctcgaggtc tatcgcaaga tgctgccgtc gatgtccgac
     1741 acggagcgcg aagctttgga agcgggcaat gtctggtggg aaggcgaact gttctccggc
     1801 atgccggagt gggaccgcct gatgtcctat ccggcaccga aactctctga cgaagagcag
     1861 gcattcctcg acggcccctg cgaaacgctg tgccgcatga tcgacgactg ggagatctgc
     1921 cacgaacgtg cggacatgcc caaagaagtc tgggattaca tcatcgagaa caggttcttc
     1981 gcgatgatca tcccaaagca gtacggcggg ctggaattct cggcctatgc gaacgcctgc
     2041 gtcatcatga agctcgcgag ccgcagcgcc acggcctcct cgacgatcgg cgtgcccaat
     2101 tcactgggtc cggccgagtt gctgctgcac tacggcaccg aagagcagaa aaaccgctac
     2161 ctccccggac tggcagccgg tacggaaatt ccctgctttg cgctgacttc gccgcaggcc
     2221 ggctcggatg cggcgtcgat catcgacagc ggcgtcgtgt gcaagggcga gtggggaggc
     2281 gagaccatcg tgggcatccg cctcaactgg gacaagcggt acatcacgct tgcgcccatc
     2341 gcgaccgtgc tcgggctggc tttcaagctc tacgacccgg accacctcat cggcgaccag
     2401 gatgaatacg gcatcacggc ggccctgatc ccgaccgaca cgccgggcgt gaccgtcggc
     2461 cggcgacatc acccgctgac catcggcttc cagaacggtc cgacttccgg taaggacgtg
     2521 ttcgtgccac tcgactacat catcggcggc cgggacatgg ccggcaaggg ctggaagatg
     2581 ctggtcgagc tgttgtccgt cggccgtgcg atcacgttac cgtcgaccgc ggcaggtggc
     2641 ggccaggcag cgagctatgc gacgggcgcc tacgccatta ttcgcaagca gttcaatacg
     2701 ccgatcgcga atttcgaggg cgtcggcgag gcgctgaccc gcatcgcagg ctacacctac
     2761 atcatgacct ccgccgtatc ggtcacgtcc ggcgcgatcg accagggcga gaagccggcg
     2821 gtaccctccg caatactgaa gtaccactgc acggagctgg gtcgcaaggt cagcaa
//
LOCUS       DATDAA010000355         4024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_235984,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000355 DATDAA010000000
VERSION     DATDAA010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235984"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VIS73_10680"
     CDS             <1..1005
                     /locus_tag="VIS73_10680"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEY5763659.1"
                     /translation="GIKELWEVDAAKHRPGLVMHTAGWPMASDTYGGGFIYHLNDRQV
                     SVGFVVGLGYRNPYLSPFEEMQRYKTHPALRDLFAGGKRIAYGARAIVAGGIQSLPKL
                     VFPGGALIGDDAGFLNASRIKGSHAAIKSGMLAADACANALAAGRAQDEITDYLRAFR
                     QSWLHDELYRARNFKPWMGKGLALGSVMVGIDQLLLRGRAPWTLHHADDSGKLEPAAE
                     CERIDYPKPDGEITFDRLSSVFLSNTNHEEDQPCHLQLADPAIPVNFNLSRYDAPEQR
                     YCPAGVYEIVRDGDGNEPRLQINAQNCVHCKTCDIKDPTQNITWVVPQGGEGPNYPNM
                     "
     gene            complement(1049..2686)
                     /locus_tag="VIS73_10685"
     CDS             complement(1049..2686)
                     /locus_tag="VIS73_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY5763660.1"
                     /translation="MDDMNQRAASGLQIWVKLVASVAILIGAAFALLMYVAASSQHDT
                     AIQQSHDFADSVNQMTMAQLLYMKVTKTYKERALYLEQVRESSGVHDLRILRSEANAY
                     QMGDDDEADQRMAPMEKAALADGKARYEVMRENGKDFLMAVIPSRNVTNYLGKDCQEC
                     HDEVKADEILGAVSMKVSLDVVNQRVSASIWKNLSVAIIVLLGMVVLVYWFVKRVVTA
                     PLQRMAQGLDEIAGGGGDLTRQLPVDSRDEIGATADSFNRMMAELRHLIAGVGRNAVE
                     VAEATRALEAQAHGIAESSQEQSEKSAGAAAATEEMVVSIGSVAESSEEVNALSQRSR
                     DGTERGQRSLGELEAQVGHVARAVNEIAATVERFVHSTESISRLTREVKEIADQTNLL
                     ALNAAIEAARAGEHGRGFAVVADEVRKLAEKSSRSASEIDEVTKTLHSESSSVNGAIA
                     DGLQVLDASREALGKVGEVLAESSEVVVQVAQGMDSISAATDEQKQAAHLVAQNVEAI
                     AQLTEDNARAVDAMTKAAKGLAQLAEGLKSDMDKFKV"
     gene            2823..3155
                     /locus_tag="VIS73_10690"
     CDS             2823..3155
                     /locus_tag="VIS73_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018993034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5763661.1"
                     /translation="MRKFSVSLFATVFGLFSTTAALALDAGAAEKLARKSGCLKCHAP
                     DKDKDGPSMKELAAKWKGKADAEEKLFKHATTKPMVKIDGKEEEHTTLKTEDAGEIRN
                     VIQWYLSH"
     gene            complement(3236..>4024)
                     /locus_tag="VIS73_10695"
     CDS             complement(3236..>4024)
                     /locus_tag="VIS73_10695"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEY5763662.1"
                     /translation="KADEVFERIIKEGLDSNLPVVASEFLGTHWPKVLKAAYLGGGQG
                     GAQWTVALQTLTDLIWSLKPKDDSDQRSELVKRLPGLLKRIAACLDRAEVSAEDREPF
                     MNCMMETHSTLMKGAQKKPHPSKEAEAKAAALCPPKPAPAVVKVVTRTVAEDGVEVEA
                     VSVDGKLKSNRPIRSSEIGNVCVGDWVEFLRRGVWVRARLSWVSPARGVMMFTNPETH
                     KALSVTPEAMALQLKSGDARMLEEGSLVDRVLDKAINSMESARR"
BASE COUNT          735 a         1333 c         1184 g          772 t
ORIGIN      
        1 ggcatcaagg aactctggga agtcgatgcg gcaaagcacc ggccgggcct cgtcatgcac
       61 acagccggct ggccgatggc gagcgatacc tacggcggcg gcttcatcta tcacctcaac
      121 gatcgccagg tatcggttgg attcgtcgtc ggcctcggct accgcaatcc ctacctctca
      181 cccttcgagg agatgcagcg ctacaagacg cacccggcgc tgcgcgatct gttcgccggc
      241 ggcaaacgaa tcgcctacgg cgcgcgggcg atcgtcgccg gcggcattca atccttgccg
      301 aagcttgtgt ttccgggcgg cgcactgatc ggcgacgatg cgggttttct caacgcctct
      361 cgcatcaagg gctcgcatgc ggcaattaag tcgggaatgc tggccgccga tgcttgcgcg
      421 aacgcgctcg ccgccggccg cgcccaggac gaaatcaccg attatctgcg ggccttccgg
      481 cagagctggc tgcacgacga actctaccgg gcacgcaatt tcaagccgtg gatgggcaag
      541 gggctcgccc tcggcagcgt gatggtggga atagaccagt tgctgctgcg cggtcgcgca
      601 ccgtggacgc tgcaccacgc cgacgacagc gggaaactcg agccggctgc cgaatgcgaa
      661 aggattgact atccgaagcc ggacggcgaa atcaccttcg accggctgtc ctcggttttc
      721 ctgtcgaaca ccaaccacga ggaagaccag ccctgccatc tgcagctggc cgacccggcg
      781 attccggtca acttcaacct atcgcgctac gacgccccgg agcagcgcta ctgcccggcc
      841 ggcgtctacg aaatcgtgcg cgacggcgac ggcaacgagc cgcgactgca gatcaacgcg
      901 cagaactgcg tgcactgcaa gacctgcgat atcaaggatc caacccagaa catcacctgg
      961 gtcgtgccgc agggcggcga gggaccgaac tacccgaaca tgtgacacct acgccgccgg
     1021 ctggccggcg gcgctgcatt ccgcggtgtc agaccttgaa cttgtccatg tcggacttga
     1081 gcccctcggc cagttgcgcg aggcccttgg ccgccttcgt catggcatcg acggcacggg
     1141 cgttgtcctc ggtcagctgc gcaatcgcct cgacgttctg agcgaccaga tgcgccgcct
     1201 gcttctgctc gtcggtggcg gcgctgatcg aatccatgcc ctgcgccacc tgaacgacca
     1261 cttcgctcga ctcggcgagc acctcgccga ccttgcccag tgcttcccgg gacgcatcga
     1321 gtacctgaag gccgtcggcg atcgcgccat tgacggagga agactcgctg tgcagcgtct
     1381 tggtcacttc gtcgatttcg ctcgccgaac gcgatgactt ctcggccagc ttgcgcacct
     1441 cgtcggcaac gaccgcgaag ccgcgaccat gttcgccggc gcgcgccgcc tcgatcgctg
     1501 cgttgagcgc gagcagattg gtctgatcgg cgatttcctt cacctcgcgc gtcagacgcg
     1561 agatcgactc tgtgctgtgc acgaaacgtt cgacggtcgc cgcgatctcg ttgacagccc
     1621 gggcgacgtg cccgacctgc gcctcgagtt cgccaaggct gcgctggccg cgctcggtac
     1681 cgtctcggct gcgctgcgac agggcattga cctcctcgct gctttcggca acgctgccga
     1741 tgctgaccac catctcttcg gtggccgccg ccgcgcctgc cgacttctcg ctctgctcct
     1801 gcgaactctc ggcgattccg tgcgcctggg cttcaagcgc gcgggtcgct tcggcaacct
     1861 cgacggcatt gcggccgacg ccggcaatca ggtgtcgcag ttccgccatc atgcgattga
     1921 aggaatcggc ggtggcgccg atctcgtcac gcgaatcgac gggcagctgg cgcgtcaggt
     1981 cgcctccgcc accggcaatt tcatccagcc cctgcgccat gcgctgcaag ggcgcagtca
     2041 ccacgcgctt gacgaaccag tacacgagca cgaccatccc cagcagcacg atgatcgcta
     2101 cgctgaggtt cttccagatc gaggcgctaa cgcgctggtt caccacgtcg agcgaaacct
     2161 tcatgctcac ggcgccgagg atttcgtcgg ctttcacttc gtcatggcat tcctggcaat
     2221 ccttgccgag gtagttcgtg acgttgcgtg acgggatgac tgccatcagg aagtccttgc
     2281 cgttctcgcg catcacctcg tagcgcgcct tgccatcggc gagcgccgcc ttttccatcg
     2341 gcgccatgcg ctggtcggct tcgtcgtcgt cgcccatctg gtaggcattg gcctcgctgc
     2401 gcaggatccg aaggtcgtgc acaccggacg actcgcgcac ctgctccaga tacagcgccc
     2461 gctccttgta ggtcttggtg accttcatgt agagaagctg tgccatggtc atctgattga
     2521 ccgaatcggc aaagtcgtgc gactgctgga tcgccgtgtc gtgctgcgaa ctggcagcaa
     2581 cgtacatgag tagtgcgaaa gcggcaccga tcagtatcgc caccgaagca accagcttga
     2641 cccagatctg cagaccactc gccgcccgtt gattcatgtc atccatttct tttttcctgc
     2701 gttgtgggat cgtgtcgatc ccttgccgcg caaatattgc cttggtgata ggaattgcct
     2761 gccgcgaaat ctatcactaa gatgccccca cctgaaacaa caaattcccc caaggagaaa
     2821 acatgcgcaa gttcagcgtt tctttattcg ctacggtgtt cggactgttt tcaacaactg
     2881 ccgcgctcgc actcgatgcc ggcgccgccg agaagctcgc gcgaaagagc ggctgcctca
     2941 agtgccacgc gccggacaag gataaggatg gaccgtcgat gaaggagctg gcggccaagt
     3001 ggaagggcaa ggcggacgcc gaggagaagc ttttcaagca cgcgacaacc aagccgatgg
     3061 taaaaatcga cggcaaggaa gaggaacaca cgacgctgaa gactgaagac gcaggcgaga
     3121 tccgcaacgt catccagtgg tacctgtcgc actagacgcg tttcactgga cgatggaatg
     3181 gaaacgggcc gcgatgcggc ccgtttgctt tttgtgtcgt cgcagccggt atgcgttaac
     3241 gccgcgcgct ttccatcgag ttgattgcct tgtcgagtac gcgatcgacc aacgagccct
     3301 cttctaacat tcttgcgtcg ccactcttca gttgcagcgc catcgcctcg ggcgtcaccg
     3361 acagtgcctt gtgggtctcg ggattggtaa acatcatcac gccacgcgcc ggactgaccc
     3421 aggacagtct ggcccggacc cagacgccgc ggcgaaggaa ttcaacccaa tcgccaacgc
     3481 aaacgttgcc aatctcgctc gaacgaatcg gccggtttga tttgagcttg ccgtcgacgc
     3541 tgaccgcctc gacctcgacg ccgtcctcgg caacggttcg ggtcactacc ttgacaaccg
     3601 ccggcgccgg cttaggcggg cacagcgcag cggccttggc ttccgcctcc ttcgatgggt
     3661 gtggcttttt ctgcgcgccc ttcatcagcg tcgaatgcgt ttccatcatg caattcatga
     3721 atggctcgcg gtcttcggca ctgacctcgg cgcgatcaag gcaggccgcg atccgcttga
     3781 gcagtccggg cagacgcttg accaactcgc tacgctggtc gctgtcatcc ttgggcttca
     3841 ggctccagat cagatcggtc agcgtctgca gcgcgacagt ccactgggcg ccaccctgtc
     3901 cgccgcccag ataggccgcc ttgagcacct tcggccagtg agtgccaaga aattccgagg
     3961 ccaccaccgg caggttcgaa tccaggcctt ccttgatgat gcgctcgaag acctcgtcgg
     4021 cctt
//
LOCUS       DATDAA010000356         2481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_270314,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000356 DATDAA010000000
VERSION     DATDAA010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270314"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /locus_tag="VIS73_10700"
     CDS             complement(<1..1517)
                     /locus_tag="VIS73_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY5763663.1"
                     /translation="MNAPSHRRPLGQVLISQGILSEDQLRIALLEQMKSNLPVGKLLV
                     KLGFVSEATLREALSESLGTQGVDLTRAIVDPSALKLVPREIAKRHHLLPLDYNAGEH
                     LFKLAVADHNDIIALDKVRSLLPEGTQIDTLLAGEGDIAKAIDQHYGYELSIDGILHE
                     IETGEIDWSSLQSSSNEYSQPVVRLIDAILTDAVKRDASDIHFEPEQNFLRVRYRIDG
                     ILRQIRSLHKSYWPAMAVRIKVMSGMNIAESRAPQDGRINLTISGRQIDFRVAAQPTI
                     HGENIVLRILDRQKGIVPLEALGLADDHLDLLKVMSGMNIAESRAPQDGRINLTISGR
                     QIDFRVAAQPTIHGENIVLRILDRQKGIVPLEALGLADDHLDLLKVMIARPEGIILVT
                     GPTGSGKTTTLYSILNHLNADSVNIMTLEDPVEYPMAMVRQTSIAETTKLDFADGIRS
                     IMRQDPDIILVGEVRDNETAQMAFRASMTGHQVFSTLHTNSAIGAIPRLLDIGILP"
     gene            complement(1534..2394)
                     /locus_tag="VIS73_10705"
     CDS             complement(1534..2394)
                     /locus_tag="VIS73_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5763664.1"
                     /translation="MAPAPARPIAVAPPATIQAVQPPAAETASSPSVQAQATEAIQAA
                     PAVRRPAPAPVASAELRAVKAANVEARRPRPSSGGASTGAQQIAVTFGTSQLDAALSM
                     GYSALNRGDLDAATIAYQEALRIEPRQLDALLGLSAIAAHKGEVDRAAAGYFRVLEID
                     PRNPSAHVGLLGLGATGDVNPRESRLRSLLSQQPVGGYEAGMLQFGLGNLYASQRRWS
                     DAQQAFFSAHASDAGNPDYLYNLAISLDHLQQHSLALRHYQAARSAAESRPGTFDRRD
                     LDARIAELSR"
BASE COUNT          428 a          734 c          850 g          469 t
ORIGIN      
        1 ggcaggatgc cgatgtccag caagcgcgga atggcgccga ttgccgaatt ggtgtgcagc
       61 gtcgagaaca cctggtggcc ggtcatcgag gcgcggaaag ccatctgcgc cgtttcgttg
      121 tcgcgcacct cgccgaccag aatgatgtcc gggtcctgac gcatgatcga gcggatgcca
      181 tcggcaaagt cgagcttggt ggtctcggcg atcgacgtct gccgcaccat cgccatcggg
      241 tattcgaccg ggtcttccag cgtcatgatg ttgacgctgt cggcgttgag gtggttgaga
      301 atcgaataaa gcgtggtcgt cttgccgcta ccggtcgggc cggtgacgag aatgatgcct
      361 tccggacggg cgatcattac cttgagcaga tcgaggtgat cgtcggcaag cccgagcgcc
      421 tcgagcggca cgatgccctt ctgacggtcg agaatgcgca ggacgatatt ttcgccatga
      481 atcgtcggtt gtgccgcaac ccggaaatcg atctgccgac cgctgatcgt caggttgatc
      541 cggccgtcct gcggcgcccg gctttcggcg atgttcatcc cgctcattac cttgagcaga
      601 tcgaggtgat catcggcaag cccgagcgct tcgagcggca cgatgccctt ctgccggtcg
      661 aggatgcgca ggacgatgtt ttcgccatga atcgtcggct gtgccgcaac ccggaaatcg
      721 atctggcggc cactgatcgt caggttgatc cggccgtcct gcggtgccct gctctcggcg
      781 atgttcattc cgctcatcac cttgatgcgg accgccatcg ccggccagta tgatttatgc
      841 aacgagcgaa tctggcgaag gatgccgtcg atgcggtaac gcacccgcag gaaattctgc
      901 tcgggctcga agtgaatgtc ggaggcgtcg cgcttgactg catcggtgag aatcgcgtcg
      961 atcaggcgca cgaccggctg gctgtactcg ttactcgagg actgcaggct ggaccagtcg
     1021 atttccccgg tttcgatctc gtgcaggatg ccgtcgatcg acagctcgta gccgtagtgc
     1081 tggtcgatcg ccttggcgat gtcgccctcg ccggcaagca gggtgtcgat ctgcgtgcct
     1141 tcgggcagca gcgaacgtac cttgtcgagg gcaatgatgt cgttgtggtc ggccaccgcc
     1201 agtttgaaca ggtgttcgcc ggcgttgtag tcgagcggca gcaggtgatg ccgtttggcg
     1261 atctcgcgcg gaacgagttt cagtgccgac gggtcgacta tggcgcgcgt gagatcgacg
     1321 ccctgcgtac caaggctttc cgatagggcc tcccgcagtg tcgcctcgga gacgaatccc
     1381 agcttcacga gcagcttacc gaccggcagg ttgctcttca tctgctcgag cagcgcgatt
     1441 cgcagctgat cctcggagag gatcccctgc gagatcagga cctggccgag cgggcgacgg
     1501 tgggagggtg cgttcatcgg atcgcggcgc agactagcgg cttaactccg cgatgcgcgc
     1561 gtcgagatcc ctgcggtcga aggtgcccgg tcgcgactcg gcggcggatc gtgctgcctg
     1621 atagtggcgg agcgcgaggg aatgctgctg caggtggtcg aggctgatcg cgaggttgta
     1681 caggtaatcg gggtttccgg catcgctggc gtgggccgaa aaaaacgcct gctgtgcgtc
     1741 gctccagcgg cgctgcgagg cgtagagatt tcccaagcca aactggagca ttcccgcctc
     1801 atagccgccg acgggctgct gggacaggag cgagcgcagg cggctttcac gcggattgac
     1861 gtcgcccgtg gcgcccaagc cgagcaggcc gacatgggcc gacggattgc gggggtcgat
     1921 ttcgaggacg cggaagtaac cagccgcggc gcgatcgacc tcgcccttgt gggcggcgat
     1981 cgccgaaagt cccaacaagg catccaactg acgaggctcg atccgcaggg cttcctgata
     2041 ggcgatcgtc gccgcgtcaa gatcgccccg gttaagtgcc gagtaaccca tcgatagcgc
     2101 ggcatcgagc tgtgaagtgc cgaaggtcac agcgatctgc tgggcacccg tcgacgcgcc
     2161 gccgctggat gggcgcggac ggcgcgcctc aacgttggcg gctttgacgg cacgcaattc
     2221 agcagacgcc actggggccg gtgcaggtcg gcgaacggcg ggcgcagcct gaatcgcttc
     2281 tgttgcctgt gcttgcacgg atggcgacga agctgtttcg gcggccgggg gctgcaccgc
     2341 ctggattgtg gccggcggtg caacggcgat cgggcgggcc ggcgcaggcg ccatggcgac
     2401 ccggcccgat ggaaccaggc gggaagccga gtcggttacc gccatgtact ggtaccaaaa
     2461 gtatccggtc acagcgagac c
//
LOCUS       DATDAA010000357        11072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_166237,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000357 DATDAA010000000
VERSION     DATDAA010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11072
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166237"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VIS73_10710"
     CDS             <1..1000
                     /locus_tag="VIS73_10710"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY5763665.1"
                     /translation="ATATGIVGAVVTLMGLLALPAMLKANYDAKLASGVIAAGGTLGI
                     LIPPSIMLIVYGATSAISVVKLYAAAMLPGILLAGLYVVYVIGRAMLNPSLAPKLPKE
                     EVSDVGFFEVTLLLMKAFFPLAFLILSVLGAILFGLATPSEAAAVGALGGVFLAAAYR
                     ALTWERLKESVFLTARTSAMVCWLFVGSWTFSSVFSYLGGEGVIKDFVLSMDLTTTTF
                     LILSQAIIFLLGWPLEWSEIIIIFVPIFLPLLPHFDVDPMFFGILVALNLQTSFLTPP
                     MAMSAYYLKGVAPPSLQLTTIFSGCLPFLAMVIVAMVIVHVFPQFVFALPEYFYGN"
     gene            1005..2366
                     /locus_tag="VIS73_10715"
     CDS             1005..2366
                     /locus_tag="VIS73_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY5763666.1"
                     /translation="MDRSPPNELSLLTATDAAEGIRAGYFTAEDLVLACLKTIDQREP
                     DVQAWTYLDPDYALKQAQQADEMRAAGVPTGPLHGVPIGIKDIIDTRDMPTEDGTALH
                     SGRRPLDDATVVSRLRAAGAVIMGKTVTTEFATYAPGKTRNPHNPAHTPGGSSSGSAA
                     AVASGMVPLALGTQTNGSVIRPASYCGVVGFKPSFGSISRTGVLRTARSLDQIGVFAR
                     TVGDAALLAQALFGHDPEDSATRPGACPPLVRVANEVPPLPPSIGMVRTPFWNDLDEA
                     AREGLLEIAELLGGRCADFPLPDSAARAIDWHRQVMESEIAANLAEDFARGAAQMSDS
                     LRTQIARGREIRAVDYLDAKSRMSLLRDALDEPFEHFHALLAPATFGAAPKGLDSTGD
                     PRLCTLWTYCGLPTLSLPMLSDEHGMPIGVQLVGRRGDDARLLRTARWLIEQVSGGAK
                     EGN"
     gene            2368..2535
                     /locus_tag="VIS73_10720"
     CDS             2368..2535
                     /locus_tag="VIS73_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763667.1"
                     /translation="MNANLVTGAIGLVLLLAFVLPPLLKLQNVALTVVALITIAMAVY
                     EFYECVRDKDN"
     gene            2540..3511
                     /locus_tag="VIS73_10725"
     CDS             2540..3511
                     /locus_tag="VIS73_10725"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEY5763668.1"
                     /translation="MKPRILVSRAVFPEVIDRLASVAEIDAHTEDAPLAPDELARRLQ
                     GKQGALTMISDRVDAAVLGDSPTLKAVCNVAVGYNNLDLDALTARGIAATNTPGVLDD
                     TTADLAWALILASTRRVVSADKWVRAGQWDGWRFLDWLGDDVHHATLGIVGMGRIGSA
                     VARRAAGFEMKVIYHNRRPLPASEASGAQWVTFPDLLATADVIVLMVPYSPATHHLIG
                     EKELAQMKPSAHLINVARGGVVDDDALIAALSARHIAGAGLDVFENEPALDRRFCDLD
                     NVVVTPHIGSASRGTRLAMAMRAADNLLQVLKGEKPRDLLNPAALAR"
     gene            3511..4422
                     /locus_tag="VIS73_10730"
     CDS             3511..4422
                     /locus_tag="VIS73_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY5763669.1"
                     /translation="MSTALELRGVTFCALKHADGTETLGLVLPEGIVDLRAAAANGTA
                     APLTLDAALQGGDRKALMRLVDACRNSTAPRLDPEQVNYGRLFRHPGKIIGIGLNYRK
                     HAREIGMDEPQNPPVFSKYDNALLAHRGAITLPPPDVASHFDYETELLIVIGRHARNV
                     AVDDALDYVAGYCTANDFSARDLQIGSPGGQWVLSKTFDGFAPIGPWFVSADQIENPN
                     ELQLQTWVNGELRQSSSTADFIFNVQQIIAYLTRHWPLEPGDIIFTGTPEGVIQGKPP
                     EQRVWLRPGDRIESDIAGLGRLQFSLQ"
     gene            4437..5933
                     /locus_tag="VIS73_10735"
     CDS             4437..5933
                     /locus_tag="VIS73_10735"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY5763670.1"
                     /translation="MDTLGHLINGQRLIAGGRTQEVFNPAEGRAEKRVELAAKSTVEQ
                     AIAAAEAAFPAWRATPPAKRARIMMRFKELLDEHAETICELITIEHGKVLADSLGELQ
                     RGIENVEYACYAPELLKGEHSRNVGPGIDSWSEFQALGVCAGITPFNFPVMVPLWMWP
                     MAVVCGNTFVLKPSERDPSSTLFVAQLAHDAGFPPGVLNLVNGDKEAVDTLLTDPRVA
                     AISFVGSTPVAEYIYRTGTSHGKRVQALGGAKNHALVMPDADIPNAVAALMGAAFGSC
                     GERCMAIPIVVAVGDETADAVVAGLREEMAKMRIGPGTHPGIDMGPLVTRAHLDKVTG
                     YIEQGVREGATLVVDGRDVSVAGHDSGYYLGPCLFDHVRPGMTIHQEEIFGPVLGIVR
                     VGTLQEGMDLIDAHEYGNGTCIFTRDGEAARYFTDHIRIGMVGVNVPLPVPVASHSFG
                     GWKRSLFGDLAIYGPDGVRFYTRRKTITQRWPSAGLREGAVFSFPSHR"
     gene            6018..6659
                     /locus_tag="VIS73_10740"
     CDS             6018..6659
                     /locus_tag="VIS73_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5763671.1"
                     /translation="METASVESTPRRRGRPPKSTATALDTRAALLRCGMELLTEKGFH
                     STGLDELLARAGVTKGSFYHCFPSKEVFGLAVIDAYDAYFAGKLDRSLLSTERQPLDR
                     IADFIADARAGMVRHHFRRGCLIGNLGQDSPALPEAYRDRLERVFLDWQARLARCLEL
                     ARDAGLIADSANCNELAEFFWIGWEGAVLRSKLMASPRPLDVFTTAFFSMLPR"
     gene            6727..7710
                     /locus_tag="VIS73_10745"
     CDS             6727..7710
                     /locus_tag="VIS73_10745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HEY5763672.1"
                     /translation="MFKAIVIDKDDAGYRAGIREVDDAELPEGNVTVDVAYSTLNYKD
                     GLAITGKGPVVRRFPMVPGIDLVGTVKSSSHPDCKPGQKVVLNGWGVGETHWGGLGQR
                     ARLNGDWLIPLPASFTPQQAMAIGTAGYTAMLCVLTLERHGVTPDKGEIVVTGAAGGV
                     GSVAVAILAKLGYTVVATTGRPTETDYLKSLGAASVIDRAELSAPGKPLGKERWAGAV
                     DVVGSHTLANVCATTRYGGVVTACGLAGGMDLPATVAPFILRGVTLAGVDSVMCPRPQ
                     RLLAWERLGRDLDPAKLGVVAHEIGLSEAIPAAADLIAGKVRGRIIVDVNR"
     gene            7716..8294
                     /locus_tag="VIS73_10750"
     CDS             7716..8294
                     /locus_tag="VIS73_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302438.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEY5763673.1"
                     /translation="MTAPISRYPVPELKDLPEDIRARILSVQEKSGFVPNVFLALAHR
                     PAEFRAFFAYHDALMEKDSSLTKAEREMIVVATSNANECQYCVIAHGAILRVRAKNPL
                     IADQVAVNYRKADITPRQKAMLDFALKVCLQAQRVDDADFARLREHGFADEDIWDIAA
                     VSAFFGMSNRMANFMSMRPNDEFHLLGRLPKA"
     gene            8416..>11072
                     /locus_tag="VIS73_10755"
     CDS             8416..>11072
                     /locus_tag="VIS73_10755"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEY5763674.1"
                     /translation="MSTRKRWLLLGGALLLLLVVALRASMPTAMSAGLRAWLEAQGAS
                     DIRIGEIRLRLLDGQLELDGLSFTAGGEESRIDSARFELEWSTTDTRRVWLRRIEIDG
                     LRTRVTLAEDGAATLAGLAPASKDKTSAGALVVMVDEASLLDGEIIVSHRGTSHRLRL
                     DQVTAGFGITTRQSAAGFSLVGAWNDIPAEISGVLASDDTRQSVQLQIDSKDLRLDEL
                     AKELETLTGKPVKLAGSATIKAALSYTAPAAKPARLLAAVDLRLADLDYGFGAGTFSA
                     RTVSWLGQIEIAGEAIALSGEIDATSPGVSTDSADSASTTQWNAGRLGWQGTLSKAGE
                     SPDWRMDGAVLVRGNVVQRDGAPLARVAEIRSSGRLQLRDSLPDYDGDLALDGLTIDM
                     PLGPASGVRAEALQWTGKVRGLADGRFEADGLLSAKNLHATRADGQGQLINIGTARAD
                     GMRLRGETISTETIALATVSVLGGDQEAAKSTSRATLQRLGIERLRFAPGALRVAALT
                     IDGLDARVIRRADGSWLPTSPLADPGDQQPADATTTAGATKPFTTQIDRLRIDESSRI
                     DFEDRAVTPPHRRTLHLKKFNVDGFDSASAKPARIDVAARIDEHAEFSAAGDWRLAAG
                     TPETDLKLSVRRLELPPLTGYTSDAIAQSIISGQLDSDTTLTIADGQLKADNALILRR
                     LELKAGTSDAGGQTDRLDLGMPLDTALRLLRDRKGDIRLQLPVRGTLTDPKFEIRDAL
                     RVATRNAMQYAALSYIKHAIQPFGTLVTLAELGAAGLNAAIRLDPIRFSTASASFDQR
                     GLDYLERLAAMLAERPKMQLRICGKASSSEFAESVTFKAQAPEQRADTLLQLAQSRAE
                     AIKRALIDVHGVGAERLFLCQPETGEDESL"
BASE COUNT         1933 a         3766 c         3453 g         1920 t
ORIGIN      
        1 tgccacggcc accggcatcg tcggcgccgt ggtgacgctg atgggcctgc tggcactgcc
       61 ggcgatgctc aaggccaatt acgacgccaa actcgcgtca ggtgtaatcg ctgctggcgg
      121 aacactcggc atcctgatcc cgccgtcgat catgctgatc gtctacggcg cgacctcggc
      181 gatctcggtg gtcaagctct acgctgcggc gatgcttccc ggcatcctgc tcgccggact
      241 gtacgtggtg tacgtcatcg gccgcgccat gctcaatcca tcgctcgcgc cgaagctacc
      301 gaaggaagaa gtttccgatg tcgggttctt cgaggtcacg ctgctgctca tgaaggcctt
      361 cttcccgttg gccttcctga ttctctccgt gcttggtgcg attctcttcg gattggcaac
      421 cccgtcggaa gccgcggccg ttggcgcgct tggtggcgtc ttcctcgcgg cagcctaccg
      481 ggcgctgact tgggaacggc tcaaggagtc ggtcttcctc accgccagaa cctcggcgat
      541 ggtgtgctgg ctgttcgtcg gctcgtggac cttttcgtcg gtgttttcct acctgggcgg
      601 cgaaggcgta atcaaggatt tcgtgctgtc catggacctg acaacgacga ccttcctgat
      661 cctctcgcag gcgatcatct tcctgctcgg ctggccgctc gaatggagcg agatcatcat
      721 cattttcgtg ccgatcttcc tgccgctgct gccgcacttc gacgtcgacc cgatgttctt
      781 cggcatcctc gttgcgctga acctgcagac ctccttcctc acgccaccga tggcgatgtc
      841 cgcgtactac ctgaaggggg ttgccccgcc aagcttgcag ctgacgacca ttttcagcgg
      901 ctgcctgccg ttcctggcga tggtcattgt cgcgatggtg atcgtgcacg tcttcccgca
      961 attcgtcttt gcgcttcctg aatatttcta tggcaactga cccaatggac cgatctcctc
     1021 ccaacgaact cagtctgctc accgccaccg atgcggccga gggcatccgt gccggttact
     1081 tcacggccga agatctggtt ctcgcctgcc tgaagaccat tgaccagcgc gaacccgacg
     1141 tccaggcgtg gacctatctc gatcctgact acgctctgaa acaagcgcag caggccgatg
     1201 aaatgcgcgc tgccggggtt cccaccggcc ccctccatgg ggttccgatc ggcatcaagg
     1261 acatcatcga cacacgcgac atgccaaccg aagacggtac tgccctgcac tcgggccgcc
     1321 gccctctcga cgatgcgacc gtagtttcgc gacttcgcgc ggccggcgcc gtcatcatgg
     1381 gcaagacggt aacgaccgag ttcgcaacct acgcgccggg caagacacgc aatccgcaca
     1441 acccggcgca cacgcctggc ggctcctcga gcggttcggc ggccgcagtg gcgtcgggca
     1501 tggtgccgct ggccctcggt acgcaaacca acggctcggt gatccgcccg gcgtcgtact
     1561 gtggtgtggt cggcttcaag ccaagctttg gcagcatttc ccggaccggc gtattgcgca
     1621 ccgcgcgcag cctcgaccag atcggggtct tcgcccgcac agtgggcgac gcagcgctgc
     1681 tggcacaggc gctgtttggc cacgaccccg aggattccgc cacccgtccg ggcgcctgcc
     1741 cgccgctggt acgcgtcgcc aacgaggtgc cgccgctacc gccgtcgatc ggcatggtgc
     1801 gcaccccctt ctggaacgat ctcgacgaag cggctcgcga aggcctgctc gagatcgccg
     1861 agttgctcgg cggacgctgc gcagactttc cgcttcctga ttctgcggca cgcgccattg
     1921 attggcatcg ccaggtcatg gaatcggaga tcgccgccaa cctcgccgag gatttcgcgc
     1981 gcggagcggc gcagatgagc gatagcctgc gcacgcagat tgcacgcggt cgcgaaatcc
     2041 gcgccgtcga ctacctcgat gcaaagtcgc gtatgtcctt gcttcgcgat gctctcgacg
     2101 agccgttcga gcacttccat gccctgctag cgccggcgac cttcggcgct gcacccaaag
     2161 gcctcgactc aaccggcgat ccgcgcctgt gcacgctgtg gacctactgc ggcctgccga
     2221 cgctgtcgct gccgatgctg agcgacgagc atggcatgcc gatcggcgtc caactggtcg
     2281 ggcgccgcgg cgacgatgcc cgcctgctgc gaaccgcgcg ctggctgatc gaacaggtca
     2341 gcggcggcgc caaggaagga aactgacatg aacgcgaacc tcgtgaccgg tgcgatcggc
     2401 ctggtgctgc tgctggcctt cgtcttgcca cccctgctca agctccagaa cgtcgcgctg
     2461 actgtggtcg ccctgatcac catcgcgatg gccgtctacg agttctacga atgtgttcgc
     2521 gacaaggaca actgacgcaa tgaagcccag gattctggtt tcccgagccg tttttcccga
     2581 agtcatagac cgtctggcat cggttgccga aatcgacgcg cataccgaag acgcaccgct
     2641 tgcgcctgac gaactggccc gccggctgca aggcaagcag ggggcgctga cgatgatcag
     2701 cgaccgcgtc gacgcagcgg tgctgggaga ctcgccgacg cttaaggcgg tctgcaacgt
     2761 cgccgtcggc tacaacaatc tcgatctcga cgcgctcacc gcgcgcggca tcgccgctac
     2821 caacacaccg ggcgtcctcg atgacaccac cgccgatctc gcctgggcgc tgatcctcgc
     2881 ctcgacccga cgtgtcgtca gcgccgacaa gtgggttcgc gccggacagt gggacggttg
     2941 gcgctttctc gactggctgg gcgacgacgt acaccacgcg acgctcggca tcgtcggcat
     3001 gggccgaatc ggcagcgccg tcgcgcgccg tgccgccggc ttcgagatga aggtgatcta
     3061 tcacaatcgc cggccgcttc ctgccagcga agcctcgggc gcgcagtggg tgacctttcc
     3121 cgacctgctg gcgacggctg acgtcattgt gctgatggtg ccctactcgc ccgccacgca
     3181 ccatctgatc ggcgagaagg aactggcgca gatgaagccg agcgctcacc tgatcaacgt
     3241 cgcccgcggc ggcgtcgtcg atgacgatgc cttgatcgcg gcgttgtcgg cacgccacat
     3301 cgctggcgcc gggctcgacg tcttcgagaa cgaaccggcg ctcgaccgcc gcttctgtga
     3361 cctcgacaac gtcgtcgtca cgccgcacat cggttcggcc tcgcgcggca cccggctggc
     3421 catggcgatg cgggctgccg acaatctctt gcaggtgcta aaaggtgaaa agccgcgcga
     3481 cctgctcaat cccgcggcgc tggcgcgctg atgtccacgg cgctagaact gcggggcgtc
     3541 accttctgcg cactcaagca tgctgacggc acggaaacac tcgggttggt actgccagaa
     3601 ggcatcgtcg acctgcgcgc tgccgcagcg aacggcaccg ctgcgccgct gacgcttgat
     3661 gccgcgctgc agggcggcga tcgcaaagcg ctgatgcggc tcgtcgacgc ctgccgaaac
     3721 agcacggcac cccgcctcga ccccgaacag gtcaactacg gacggctctt ccggcacccg
     3781 ggcaagatca tcggcatcgg ccttaactac cgcaagcacg cgcgcgagat cggcatggac
     3841 gaaccgcaga atccgccggt gttctccaag tacgacaacg ccttgctcgc ccatcgcgga
     3901 gcgatcaccc tgcccccacc cgatgtcgca agccacttcg actacgagac cgagctgctg
     3961 atcgtgatcg gccgccacgc gcgcaatgtc gccgtcgacg acgcgctcga ctacgtcgcc
     4021 ggctactgca cggccaacga cttctcggcg cgcgacctgc agatcgggtc acccggcggc
     4081 cagtgggtac tgtcgaaaac gttcgatgga tttgcaccga tcggcccctg gttcgtcagc
     4141 gccgaccaga ttgagaaccc caacgaattg cagctgcaga cctgggtcaa tggcgaattg
     4201 cgccagtcgt catcgacggc cgatttcatc ttcaacgttc agcagatcat tgcctacctg
     4261 actcgtcact ggcccctgga accgggtgac atcatcttca cgggcacacc ggaaggcgtg
     4321 attcagggca agcctcccga gcaacgtgtc tggctgcggc ctggcgaccg catcgagagt
     4381 gatatcgccg gactcggccg cctgcagttt tcactgcagt aaggggaggt gtattcatgg
     4441 atacgctcgg acatctgatc aacggccagc gactgattgc cggtggtcgc acgcaggagg
     4501 tcttcaatcc ggcggaaggt cgcgctgaga agcgcgtcga gctggcagcg aagagcaccg
     4561 tcgagcaggc catcgccgca gccgaagcgg cattcccggc ctggcgggca acgccgccag
     4621 caaagcgcgc ccgaattatg atgcgtttca aggagctgct cgacgagcac gccgagacga
     4681 tctgcgagct gatcacgatc gaacacggca aggtactcgc cgactcgctc ggcgagttgc
     4741 agcgcggcat cgagaacgtc gaatacgcct gctatgcgcc cgagttgctc aagggcgaac
     4801 acagccgcaa cgtcggtccc ggcattgact cctggagcga gttccaggcg ctcggcgtct
     4861 gtgccggcat caccccattc aacttcccgg tgatggtgcc actgtggatg tggccgatgg
     4921 cagtggtgtg cggcaacacc ttcgtcctca aaccctccga acgcgacccg tcgtcgacct
     4981 tgtttgtcgc ccagctggcg cacgatgccg gctttccgcc cggcgttcta aacctcgtca
     5041 acggcgacaa ggaggcggtc gacacgctgc tcaccgatcc gcgcgtggcg gcgatcagct
     5101 ttgtcggctc gacaccggtt gccgagtaca tctaccgcac cggcactagc cacggcaaac
     5161 gcgtgcaggc actgggaggc gcaaagaacc acgcactggt gatgcccgat gccgatattc
     5221 cgaacgccgt cgcagcgctg atgggcgccg cctttggctc ctgcggcgag cgttgcatgg
     5281 cgattccgat cgtcgtcgcg gtcggcgacg agaccgccga cgcggtcgta gccggtctgc
     5341 gcgaagaaat ggctaagatg cgcattggcc cgggcaccca tccggggatt gacatgggtc
     5401 cactggtaac gcgcgcccac cttgacaagg tcaccggcta catcgaacaa ggcgtgcggg
     5461 aaggcgcgac cttggttgtt gacggccggg acgtgagcgt cgccggccac gattcgggct
     5521 actacctcgg cccctgcctg ttcgaccatg tccgccccgg aatgacgatt caccaggaag
     5581 aaatcttcgg cccggttctc ggcatcgtcc gcgtcggcac cctgcaggaa ggcatggacc
     5641 tgatcgatgc ccatgagtac ggtaacggga cctgcatctt cacccgcgac ggcgaggcgg
     5701 cgcgctactt caccgaccac atccggatcg gcatggtcgg cgtcaatgtg cccttgccgg
     5761 ttccggtagc cagccactcc ttcggcggct ggaagcgatc gctgttcggc gacctggcaa
     5821 tctacggccc tgacggagtg cgcttctata ccaggcgcaa gaccatcacc caacgctggc
     5881 catcggccgg cctgcgcgaa ggcgcggtgt tcagcttccc cagccatcgc tgacggaacg
     5941 gtggtcgcgg gggatcggca acagcctttc tgaaacgcca ccttgcgtta ataaaccatt
     6001 cggtctatta ttgaccgatg gaaactgcat ccgtcgaatc gacaccccgc cggcgtgggc
     6061 gaccgcccaa gtccaccgcc acggcgctcg acacgcgggc ggccctgctc cgttgcggca
     6121 tggaattgct gaccgagaag ggatttcaca gcaccggact cgacgagctg ttggcgcggg
     6181 ccggcgtcac caagggcagc ttctatcatt gctttcccag caaggaggtc tttggcctgg
     6241 cggtaatcga cgcctacgat gcctacttcg ccggcaaact cgatcgctcg ctgctgagca
     6301 cagaacgtca gccgctcgac cgcatcgccg atttcatcgc cgacgcacgc gccggcatgg
     6361 tgcgccacca cttccgccgc ggctgcctga tcggcaatct cgggcaggac tcaccggcgc
     6421 tgcccgaggc ctaccgggac cgcctcgaac gggtctttct tgactggcag gccagactcg
     6481 cgcgttgcct ggaacttgcg cgcgatgccg gcttgatcgc ggactcggcg aactgcaacg
     6541 aactcgccga gttcttctgg atcggctggg agggagcggt actgcgcagc aagctgatgg
     6601 cctcgccgcg accgctcgac gtattcacca ccgccttttt ctcaatgctg ccgcgctgac
     6661 cgacgatcca tcgaccggcg ttgcggcctt tcgcacacca catccattca cagaggagaa
     6721 ctacacatgt tcaaggcaat cgtaatcgac aaagacgacg cgggttaccg ggccggcatt
     6781 cgcgaggttg atgatgccga gttacccgag ggcaacgtca cggtcgacgt cgcctattcg
     6841 acactcaact acaaggacgg tctggcgatc accggcaagg gtccggtcgt gcgccgcttc
     6901 ccgatggtgc cgggaatcga cctggtcggc accgtcaaga gcagcagcca tcccgactgc
     6961 aagccggggc agaaggtcgt cctcaacggc tggggcgtcg gcgaaacgca ctggggcggc
     7021 cttggccagc gcgcccgcct caacggcgac tggctgatcc cgctgcccgc ctcgttcacg
     7081 ccgcaacagg cgatggcgat cggtaccgcc ggttacacgg cgatgctctg cgtgctcacg
     7141 ctcgaacgcc acggtgtgac gccggacaag ggcgaaatcg tcgtcaccgg cgccgccggc
     7201 ggtgttggca gcgttgccgt cgccatcctc gccaagctcg gctacacggt cgtcgccacc
     7261 accgggcggc cgacggaaac cgattatctg aagtcgcttg gcgccgccag cgtcatcgat
     7321 cgcgcagagc tctcggcgcc cggaaaaccg ctgggtaagg agcgctgggc cggtgccgtc
     7381 gacgtcgtcg gctcgcacac gctcgccaat gtctgcgcga caacacgcta tggcggcgtc
     7441 gtcaccgcct gcggcctagc cggcggcatg gacctgccgg cgacggtcgc acccttcatc
     7501 ctgcgcggcg tcacgctcgc cggtgtcgac agcgtcatgt gcccgcgccc gcagcgactc
     7561 cttgcctggg agcgcctggg ccgcgacctc gatccggcca agctcggtgt agtcgcccac
     7621 gaaatcgggc tttcagaggc aattccggca gcggccgacc tgattgccgg aaaggtgcgc
     7681 ggccggatca tcgtcgacgt caatcgctga tcgctatgac cgctccgatc agtcgttacc
     7741 cggtgcccga gcttaaggat ctgcccgagg acatccgggc acgcatcctg agcgttcagg
     7801 agaagtcggg tttcgtgccc aacgtcttcc tcgcgctggc gcaccggccc gccgagttcc
     7861 gcgcgttctt cgcctaccac gatgcgctga tggagaagga cagcagcctc accaaggccg
     7921 agcgcgagat gatcgtcgtc gccacctcga acgccaacga gtgccagtat tgcgtaatcg
     7981 cccacggcgc gatcctgcgc gttcgcgcga agaatccgct gatcgccgat caggtggcgg
     8041 tgaactaccg caaggcggac atcacgccgc ggcagaaggc gatgctcgac ttcgctttga
     8101 aggtctgcct gcaggcgcag cgagtcgatg atgccgattt cgccagactg cgggagcacg
     8161 gttttgcgga cgaagacatc tgggatatcg cagccgtctc ggctttcttc ggtatgagca
     8221 accggatggc caatttcatg tcgatgcggc cgaacgacga gttccacctg ctcgggcgac
     8281 tgcccaaggc ctgaccgaaa ccgcgtcgat tcgtcatgcg acaatgacgc atcggcgcgc
     8341 atgcccgtgc cttggcgctg cccatcgcgc cacgcctctt ctggctgcgc cgcccgtact
     8401 caaggaacca ctatcgtgtc tacccgcaag cgctggctgc ttctcggcgg cgcactcctg
     8461 ctgctgctcg tcgtcgccct gcgtgcgagc atgccgaccg cgatgtcggc aggactgcgc
     8521 gcctggctcg aggcgcaggg tgccagcgat atcaggatcg gcgagatccg actccgcctg
     8581 ctcgatggcc agcttgaact cgatgggctg agcttcaccg ccggtggtga ggaatcccga
     8641 atcgacagcg cgcgcttcga gcttgagtgg tcgaccaccg atacgcgccg ggtctggctc
     8701 aggcgcatcg agattgacgg actgcgaaca cgcgtgacgc ttgctgagga cggcgcggcc
     8761 accctcgccg gccttgcgcc ggccagcaag gacaagacga gcgccggcgc cctcgtggtg
     8821 atggttgatg aggccagcct gctcgatggc gaaatcatcg tcagccaccg cggcaccagt
     8881 caccgcctcc gcctcgacca ggtgacggcc ggcttcggca tcaccacccg gcagtcggct
     8941 gccggcttca gcctggtcgg cgcctggaac gacatcccgg ccgaaatcag tggggtactg
     9001 gccagcgacg atacgcgcca atcggtgcag ttgcagatcg acagcaagga tcttcgtctg
     9061 gatgagttgg cgaaagagct ggagacgctg accggcaagc cggtgaaact cgccggcagc
     9121 gcgacgataa aggccgcgct gagctatacg gcgccagccg ccaagccggc gcgcctgctg
     9181 gccgccgtgg acctgcggct cgcggatctc gactacggtt tcggggcggg caccttcagc
     9241 gcccgcacgg tcagctggct cggacagatc gagatcgccg gcgaggccat cgcgctgagc
     9301 ggcgaaatcg acgccacctc acccggcgta tctactgaca gcgcggattc cgcttcgaca
     9361 acgcaatgga atgccggtcg cctgggctgg caaggcacac tgagcaaggc cggagaatcg
     9421 cccgactggc gcatggacgg agccgtgttg gtgcgcggta acgtcgtcca gcgtgatggc
     9481 gcgccgctgg cccgtgtcgc ggaaatccgc agcagcgggc gcctgcaact acgcgattcg
     9541 ctaccggatt acgacggcga cctggcgctg gacggcctga cgatcgacat gccgctcggc
     9601 ccggcctccg gcgtccgcgc cgaggcactg caatggacag gcaaggtccg cgggcttgcc
     9661 gatggtcgct ttgaggccga tggcttgctt tcggcaaaga acctgcatgc cacccgcgcc
     9721 gatggccagg ggcagctgat caacatcggc accgcccgcg ccgatggcat gcgactgcgc
     9781 ggcgagacga tctcgaccga aaccattgcc ttggccaccg tatcggtgct cggtggcgac
     9841 caggaggcgg ccaaatccac cagtcgcgcg accctgcagc gcctcggtat cgagcgcctg
     9901 cgcttcgcgc cgggcgcgct gcgcgtcgcc gcgttgacca tcgatggact cgacgcccgg
     9961 gtgattcgcc gcgccgacgg aagctggctg ccgacgagtc cgcttgccga tcccggcgac
    10021 cagcaacccg cggatgcgac cacgacagcc ggcgcaacga agccctttac cacgcagatc
    10081 gatcgcttgc gtattgacga atcgagccgg attgatttcg aggaccgcgc cgtaacaccg
    10141 ccgcaccggc gcacgcttca cctgaaaaaa ttcaacgtcg acggcttcga cagcgcctcg
    10201 gccaaaccgg cgagaatcga tgtcgctgcc cgcatcgacg agcacgccga gttcagcgcg
    10261 gccggcgatt ggcggctggc ggccggcact ccggaaacgg atctcaagct ctctgtcagg
    10321 cgtcttgagt tgccaccgct gactggctat accagcgatg cgatcgctca gtcgattatc
    10381 agcgggcagc tcgacagcga tacgaccttg acgatcgccg atggccagct caaggcagac
    10441 aacgcgctga tcctgcgtcg gctcgaactc aaggccggca ccagcgatgc cggcggccaa
    10501 accgaccgcc tcgacctcgg catgccgctg gacacggcgc tgcgcctgct gcgcgaccgc
    10561 aagggcgaca tccgtttgca gttgccggtt cgcggcaccc tcaccgaccc gaagttcgag
    10621 atccgcgatg cgttgcgcgt ggcaacgcgc aacgcgatgc agtacgcggc gctctcctac
    10681 atcaagcatg cgatccagcc cttcggaacg ctggtaacgc ttgccgagct cggcgcggcc
    10741 ggtctcaatg ccgcgattcg cctcgatccg atacgtttct cgacggccag cgccagcttc
    10801 gaccagcgcg ggctggatta tctggaacgc ctcgcggcga tgctcgcgga gcggccgaag
    10861 atgcagctgc gcatctgtgg caaggcgagc agcagcgagt tcgccgagag cgtcacgttc
    10921 aaggcgcagg cgcccgaaca acgcgccgac acactgctgc agctcgccca gtcgcgcgcc
    10981 gaggcgatca agcgggcgct gatcgatgtc cacggcgtcg gcgccgagcg cctgtttctc
    11041 tgccagcccg aaaccggaga agacgagtcg ct
//
LOCUS       DATDAA010000358         2758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_25989,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000358 DATDAA010000000
VERSION     DATDAA010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25989"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VIS73_10760"
     CDS             <1..696
                     /locus_tag="VIS73_10760"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY5763675.1"
                     /translation="ICWATKGFELHTGKLPHVVAAEVLGAERPVAVLSGPTFAREVGD
                     GLPTALTIAANDAGFAQDLAVALSGENFRAYTSDDMIGVEVGGAVKNVLAIGAGMSDG
                     LGFGANSRVALITRGLVEMTRLGVALGARRETFMGLAGMGDLVLTCTDNLSRNRRMGL
                     ALAAGKSVDEAQEEIQQVVEGVLAAQAVHEVAEKLGIEMPICHQVYRILYAGASPREA
                     VGALMRRDLKSEH"
     gene            793..2268
                     /locus_tag="VIS73_10765"
     CDS             793..2268
                     /locus_tag="VIS73_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5763676.1"
                     /translation="MDRRQFCRSAVAAGVTAAFLPGCGKETPVATRADTGIAAVSLDG
                     AEIELSKAAIREFGDAMSGPVMLSGHPQYDSVRMIWNGMHDKRPALIARCLNAEDVSN
                     AVSFARDHDVLVAVRGGGHSWPGKSVCDGGLMIDLSPMHNANADADTRRARVEGGALL
                     NNLDTAALEKGMVTTAGVVSHTGCGGFTLGGGFGRLNRKYGLTIDNVTGAQIVTADGR
                     IRRISANEEPDLFWAIRGGGGNFGVVTEFEYQLHPFDRNVLSGNIVWPVEQARDVLEF
                     YAEWSAGLSDELYVGPSLARIPDAGDSVIMEVVYNGDPAVGEKELAPLRSIGKPTVDG
                     VTVQDYMIMQTQEDATLAHGVRSYAKNGMVKEWSQGLVDTMIDAYDPRVFLTNHVAGG
                     AVKRVGETDTAFPHRNAELMLVFVTGWGDPAMDQEMIQATRAQYAAIESYMGGYYDNI
                     EFDGDEAAGNYGPAYSRLSKIKGRYDPGNLFRLNSNIEPAA"
     gene            complement(2269..>2758)
                     /locus_tag="VIS73_10770"
     CDS             complement(2269..>2758)
                     /locus_tag="VIS73_10770"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEY5763677.1"
                     /translation="MGCGILNDMFSLILYQPEIPPNTGNIIRLCANTGVGLHLVEPLG
                     FELDEPRLRRAGLDYREFANVQVHGSLGDALDVLGNPRLFALSTRGSTRYDTPLYRRG
                     DAFLMGPETRGLPQALLDSLPRERRLRLPMRSGNRSLNLSNAAAVVVYEAWRQLGFEQ
                     GT"
BASE COUNT          512 a          856 c          912 g          478 t
ORIGIN      
        1 atctgctggg cgaccaaggg tttcgaactg cataccggca agctgccgca cgtcgttgcc
       61 gccgaggtgc tgggcgccga gcgaccggtc gcggttttgt ccggcccgac cttcgccagg
      121 gaggtcggcg acggcctgcc gacggcactg accatcgccg cgaacgacgc gggttttgcg
      181 caggatctgg cggtagccct ctccggtgaa aattttcgcg cctatacctc cgacgacatg
      241 atcggtgtcg aggtgggcgg cgccgtcaag aacgtgctgg ccatcggcgc gggcatgtcc
      301 gatggcctcg gcttcggcgc gaactcccgc gtagccctga tcacccgcgg cttggtcgag
      361 atgacgcggc tgggagtggc gcttggcgca agacgcgaaa ccttcatggg tctggcgggc
      421 atgggcgacc tcgtgctcac ctgtacggac aacctctcgc gcaaccggcg catgggcctc
      481 gcgctggcag cgggcaaatc ggtcgatgaa gcccaggaag agatccagca agtcgtcgag
      541 ggcgtgctgg ccgcccaggc cgtgcacgag gttgccgaga aactcgggat cgaaatgccg
      601 atctgccacc aggtgtatcg gatactgtat gcgggcgcat ccccgcgcga agccgtgggc
      661 gcactgatgc gccgggacct gaaatcggag cattgagacc gcctcggctc gctattcgcc
      721 accgatcctc taaacttttt ccctatacag cgctatttgc tgtaagcgct tcaaaaatat
      781 aggggaacaa tgatggacag acgtcaattc tgccggtccg cggtcgccgc cggcgtcacc
      841 gccgcattcc tgccgggctg cggcaaggag acaccggttg ctacccgggc ggatacgggc
      901 atcgccgccg tgtccctgga cggtgccgag atcgaactga gcaaagccgc catcagggag
      961 ttcggcgatg ccatgtccgg gcccgtgatg ctctccgggc atccacagta cgactcggta
     1021 cgtatgatct ggaatggcat gcacgacaag cgcccggcgc taatcgcccg gtgcctgaat
     1081 gcggaggatg tcagcaatgc cgtgagtttc gcgcgtgacc acgacgtgct cgttgcggtg
     1141 cgcggcggcg gccacagctg gcccggcaag tcggtctgcg acggcgggct gatgatcgac
     1201 ctgtcaccga tgcacaacgc caatgccgac gcggacacgc gccgggcccg cgtcgagggg
     1261 ggtgcgttgc tcaataacct cgatacggct gcactcgaga aaggcatggt gaccaccgcc
     1321 ggtgtcgtct cccacaccgg ctgcggcggg tttacgctgg gcggtggctt cggccggctc
     1381 aatcgcaaat acggcctgac gatcgacaac gtcacgggcg ctcagatcgt caccgccgac
     1441 ggccggatcc ggcgcatcag cgcgaacgaa gagcccgatc tcttctgggc gattcgtggc
     1501 ggcggcggca atttcggcgt ggtaacggaa ttcgaatacc agctgcaccc gttcgaccgc
     1561 aatgtgctat ccggcaatat cgtctggccg gttgaacagg cgcgggacgt gctcgaattc
     1621 tacgcggaat ggtcggccgg cctctcggat gagctgtacg tcggaccctc cctggcaagg
     1681 attccggacg ccggggactc cgtgatcatg gaagtcgtgt acaacggcga tcccgccgtt
     1741 ggcgaaaagg aactggcccc gctgcgctct attggcaaac cgaccgttga cggcgtcacg
     1801 gtgcaggact acatgatcat gcagacgcag gaagacgcta ccctcgcgca tggcgtccgt
     1861 tcctacgcca agaacggcat ggtgaaggag tggtcgcagg gactcgtcga tacgatgatc
     1921 gacgcctacg atccgcgggt gttcctgacc aaccacgtcg ccggcggcgc cgtgaagcgc
     1981 gtcggcgaga ccgacaccgc gttcccgcat cgtaatgccg agctcatgct cgtgttcgtc
     2041 acgggctggg gcgatccggc catggaccag gagatgatcc aggcaacgcg cgcgcaatac
     2101 gccgcgatcg aatcctacat ggggggctat tacgacaaca tcgagttcga tggcgacgaa
     2161 gcggccggca actacggacc ggcttactcg cgattatcga agatcaaggg gcggtacgat
     2221 ccgggcaacc tgttcaggct gaacagcaac atcgagcctg cggcctgatc aggtgccctg
     2281 ctcgaagccg agctgccgcc aggcttcgta gacgacaacc gcggcggcat tcgacaggtt
     2341 gaggctgcgg ttgcccgatc gcatgggcag ccgcaacctt cgttcccgcg gcaggctgtc
     2401 gagcagcgcc tggggcaggc cacgtgtttc cgggcccatc aggaacgcgt cgccgcgccg
     2461 gtaaagcggc gtgtcgtagc gggtgctgcc gcgggtgcta agcgcaaaaa ggcgcggatt
     2521 gcccagcacg tcaagcgcat ccccgagcga tccatggacc tgtacgtttg cgaattcccg
     2581 gtagtcgaga ccggcccggc gtaagcgcgg ttcgtccagt tcgaaaccca gtggctccac
     2641 gaggtgcaga cccacgccgg tgttcgcgca aaggcgaata atgttgccgg tgttgggcgg
     2701 aatttccggc tggtacagga tcaggctgaa catatcgttt agaatgccgc atcccatg
//
LOCUS       DATDAA010000359         4044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_236136,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000359 DATDAA010000000
VERSION     DATDAA010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236136"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VIS73_10775"
     CDS             complement(<1..678)
                     /locus_tag="VIS73_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEY5763678.1"
                     /translation="MSTTSQRVVVDPITRIEGHLRIEAETDADGRITRASSAGTMVRG
                     IEIVLRGRDPRDAWAFAQRICGVCTLVHGIASVRSVENALKIEIPKNAELIRNLMIGA
                     QYIHDHVMHFYHLHALDWVDVVSALKADPKATSELAQSISGYAKSSPGYFADVQKKLK
                     NFVEAGQLGIFAGGYWGHPAYRLPPEANLMAVAHYLDALVWQREVVKLHAIFGGKNPH
                     PNFLVGGA"
     gene            complement(697..1968)
                     /locus_tag="VIS73_10780"
     CDS             complement(697..1968)
                     /locus_tag="VIS73_10780"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY5763679.1"
                     /translation="MTATLAKGVNAFREVIDAPIASFFSSCVHCGLCAEACLFYTETG
                     DPKYTPIHKLEPLRRVWQQEYTALGRLTGLLGLNQPITDEELAEWSPLVYDSCTMCGR
                     CAMVCPVGNDLTYMIRKMREGMAIAGHAPEGLVTATTRAITAGSPAGVKWVTVQAQIK
                     RMEKQFGVTVPVNQENVDYLVLMSSWEILNYPEYLGAVGRIFRQAGKTWTLAEDAYEA
                     TNSGIQIGVSDLAKELVIRIVTAAETLKAKCVISPECGHAYTALRWDGPNLIGRPYGF
                     KVQHILEVLDELRAEGKLKLKPPQDGMTQPLTYHDPCQIARRGGVVEQPRRLLELIAT
                     DFREMAESGTMNWCCGGGGGVSANERAEPLRIVAFKKKKSQIEALGVNTLITACANCR
                     VVMEEAIEHYGMKVEMLGLTEVVAEQLVDEK"
     gene            complement(1965..2624)
                     /locus_tag="VIS73_10785"
     CDS             complement(1965..2624)
                     /locus_tag="VIS73_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763680.1"
                     /translation="MSNLELLTLARGPILDVALLIFAAGVVLRGFEVFSLGRKADLSP
                     ARPDSPGSGWQTILSRSIPPPGMINRAPATYIAGYIFHLGLFATLFLFAPHIEIIRDL
                     TGLSWPALPSGVIDAIAVASIIALIVLLVDRLNNPVKRLLSGLGDYLAWTLTLLPLVT
                     GYLAVNHLLLEYTLMLAIHVLSVAALLALLPFTKLFHTFSVFASRWYNGNIFGRKGVA
                     S"
     gene            complement(2621..3769)
                     /locus_tag="VIS73_10790"
     CDS             complement(2621..3769)
                     /locus_tag="VIS73_10790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HEY5763681.1"
                     /translation="MKLPHPGRQPAKEPSPLLDQPGSVGDGLAIYGVSRREFMKFCAS
                     VTAALSLPATMRPAIAAALSTARRQSVIWLSFQECTGCTESITRSHSPTIENLIFDLI
                     SLDYHHTLQAASGDAAEHAREAAMREHAGKYLLIVDGSIPMGNPGYSTIAGISNHDML
                     LETARDAAAIVAVGTCAAFGGIPKANPNPTGAVSVGKLIKGKPIVNVPGCPPIPAVMT
                     GVLAHFLTFGQLPELDDSGRPKVFYGETIHDRCYRRPFYDQGKFAETFDDEGARRGWC
                     LYKLGCRGPVTYNACATSKWNDGTSFPIQSGHGCLGCSEANFWDAGSFYKALSVPITP
                     AQKTLSTGAAVGLAAGVAVTWFNRRQKAAAKAQHQPAEVADLTRGDES"
BASE COUNT          690 a         1204 c         1375 g          775 t
ORIGIN      
        1 cgcgccgccg acgaggaagt tagggtgcgg attcttgccg ccgaagattg cgtgcagctt
       61 gaccacctcg cgctgccaga ccagtgcatc gaggtaatgc gcgaccgcca tcaggttggc
      121 ttccggcggc agcctgtacg ctgggtgccc ccagtagccg ccggcgaaga tgccgagctg
      181 gccggcctcg acgaagttct tcagtttctt ctgcacgtcg gcgaagtagc cgggcgagga
      241 cttcgcgtag ccggagatgc tctgcgcgag ttcggaagtt gccttggggt cggccttcaa
      301 tgccgagacg acgtcgaccc agtcgagggc gtgcaggtga tagaagtgca tcacgtggtc
      361 gtggatgtac tgcgcgccga tcatcaggtt gcggatcagt tcggcgttct tcgggatctc
      421 gatcttcagt gcgttctcga ccgagcgtac cgaggcgatg ccgtgcacca gcgtgcacac
      481 gccgcagatg cgttgggcga aggcccaggc gtcgcgcggg tcgcggccgc gcagcacgat
      541 ctcgatgccg cggaccatgg tgccggccga ggaggcacgg gtgatgcgac cgtcggcatc
      601 ggtttcggct tcgatgcgca ggtggccttc gatgcgcgtg atcggatcga cgacgacgcg
      661 ttggctggtg gtgctcatat cagttctcct cggcccttat ttctcgtcaa ccaactgttc
      721 ggcgacgact tcggtcaggc cgagcatctc gaccttcatg ccgtagtgct cgatggcttc
      781 ttccatgacg acgcggcagt tggcgcaggc ggtgatcagc gtgttcacgc caagcgcctc
      841 gatctgtgac ttcttcttct tgaaggcgac gatgcgcagc ggctcggcac gttcgttggc
      901 ggagacgcca ccgccgccgc cgcagcacca gttcatcgtt ccgctctccg ccatctcgcg
      961 gaaatcggtg gcgatcagtt cgagcagacg ccgcggctgc tcgacgacgc cgccgcggcg
     1021 ggcgatctgg caggggtcgt ggtaggtcaa cggctgggtc atcccgtcct gcggcggctt
     1081 gagcttgagc ttgccttcag cgcgcagttc atcaagcact tcgaggatgt gctgaacctt
     1141 gaagccgtag gggcggccga tcaggttggg gccgtcccag cgcaatgccg tgtaggcatg
     1201 gccgcactcg gggctgatca cgcacttcgc cttgagcgtc tcggcggcgg tgacgatgcg
     1261 aatgacgagt tctttggcga gatcggagac gccgatctgg atgccgctgt tggtcgcctc
     1321 gtaggcatcc tcggccagtg tccaggtctt gcccgcttgc ctgaagatcc gaccgacggc
     1381 accaaggtat tcgggatagt tgaggatctc ccacgaggac atcagcacca gatagtcgac
     1441 gttctcctga ttgacgggaa ccgtcacgcc gaactgcttc tccatgcgct tgatctgcgc
     1501 ctggacggtg acccatttca cgccggccgg gctgcccgcg gtgatcgccc gcgtggttgc
     1561 cgtgactagg ccctccgggg cgtggccggc gatcgccatg ccttcgcgca tcttgcggat
     1621 catgtaggtg aggtcgttgc cgaccgggca gaccatcgcg caacggccgc acatcgtgca
     1681 gctgtcgtag accagcgggc tccactcggc cagttcctcg tcggtgatcg gctggttgag
     1741 cccgagcagg cccgtgagcc tgcccagcgc cgtgtactcc tgctgccaga cgcggcgcag
     1801 cggttcgagc ttgtggatcg gcgtgtattt cggatcgccg gtttcggtgt agaagaggca
     1861 ggcctcggcg cacagcccgc aatgcacgca gctcgagaag aagctggcga tcggggcgtc
     1921 gatgacttcc cggaaggcgt tgacgccttt ggcgagggtc gcggtcacga tgccactcct
     1981 tttcgcccga agatgttgcc gttgtaccag cgcgaggcga agacgctgaa ggtgtggaac
     2041 agtttggtga atggcagcag cgccagcagc gccgcgaccg agagcacgtg gatcgccagc
     2101 atcagcgtgt actcgagcag cagatggttg acggcgagat agccggtgac cagcggcagc
     2161 agcgtcaggg tccacgcgag gtagtcgcct aggcccgaga gcaggcgttt caccgggttg
     2221 ttgaggcggt cgaccagcag cacgatcagc gcaatgatcg aggcgacggc gatcgcgtcg
     2281 atgacgcccg aaggcagcgc cggccaactg agaccggtga ggtcacggat gatctcgatg
     2341 tgcggcgcaa agaggaacag cgtcgcgaac agcccgaggt gaaagatgta accggcgatg
     2401 taggtggccg gcgcgcggtt gatcatgccg ggcggcggga tcgagcgcga caggatcgtc
     2461 tgccagccgc ttcccggaga atcgggacgg gccggcgaga gatcggcctt gcggcctagg
     2521 ctgaagacct cgaaaccacg caggacgacg ccggcggcga agatcagcag cgcaacgtcg
     2581 aggatcggcc cgcgcgccag cgtcagcagt tcgagattgc tcatgattcg tctccccgcg
     2641 tcaggtccgc gacttcagcc ggctggtgct gcgcctttgc cgcggccttc tgccgtcggt
     2701 tgaaccaggt caccgcgaca ccggcggcga ggccgacggc ggcaccggtc gacagcgtct
     2761 tctgcgccgg cgtgatcggc accgagagcg ccttgtagaa ggaaccggca tcccagaagt
     2821 tcgcttccga gcagccgagg caaccgtgac ccgactggat cgggaagctc gtgccatcgt
     2881 tccacttgct ggtcgcgcag gcgttgtagg tcaccgggcc gcgacagccc agcttgtaga
     2941 gacaccagcc acggcgcgcg ccttcgtcgt cgaaggtttc ggcgaacttg ccttggtcgt
     3001 agaaggggcg gcgataacag cggtcgtgga tggtctcgcc gtagaagacc ttcgggcgcc
     3061 cggagtcgtc gagttcgggc agctggccga aggtcaggaa gtgcgccagc actccggtca
     3121 tcaccgcagg gatcggtgga cagcctggca cattcacgat cggcttgccc ttgatcagtt
     3181 tgccgaccga gaccgcgccg gtcggattgg ggttggcctt gggtatgccg ccaaaggcgg
     3241 cgcaggtgcc tacggcgacg atggctgcgg catcgcgggc ggtttcgagc agcatgtcgt
     3301 gattgctgat gccggcgatc gtcgaataac ccgggttgcc catcggaatc gagccgtcga
     3361 cgatcagcag gtacttgccg gcgtgctcgc gcatcgctgc ttcgcgtgcg tgttccgctg
     3421 cgtcgcccga ggccgcctgc aacgtgtggt gatagtcgag tgagatcagg tcgaagatca
     3481 ggttctcgat cgtcggcgaa tgcgagcggg tgatcgactc ggtgcagccc gtgcattcct
     3541 ggaaggagag ccagatcacc gattgccgcc tggcggtcga cagggcagcg gcgatcgctg
     3601 gtcgcatagt ggcgggtagc gatagcgccg ccgtcacgct ggcacagaac ttcatgaatt
     3661 cacggcggct tacaccgtag attgccagtc catcgccgac gctaccgggc tgatcaagca
     3721 gcgggcttgg ctccttggcg ggttgcctgc ccgggtgcgg cagtttcatg tcggcctccc
     3781 ctgttttgag cggacgtttc agatggatca ctgcactcat tgataggcgt ctgcgaccaa
     3841 cgccttcttg acctcgatca aggacgaata agtaattgct gaaagactta ggccgactcg
     3901 ccagatgaga actatttttg atttcacgac gactctggcg tcaggcgctt ggtcgctatt
     3961 accaaagtgc gattgaaggc gtgtgggccg gcgagtagag tatttctacg cgagccaaag
     4021 attatttttt cgcttcattt gcga
//
LOCUS       DATDAA010000360         7300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_96118,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000360 DATDAA010000000
VERSION     DATDAA010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96118"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1371
                     /gene="fabF"
                     /locus_tag="VIS73_10795"
     CDS             133..1371
                     /gene="fabF"
                     /locus_tag="VIS73_10795"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY5763682.1"
                     /translation="MTRRRVVITGLGVVSPVGNTVADAWKNILAGVSGIGMISRFDTT
                     TFSSRIAGEVRDFDIGQYLSPKEARRMDRFIHYGMAAGIQAVRDAGLEASPADAERIG
                     VAIGSGIGGLPMIEDTKDEYIAGGVRKVSPFFVPGSIINMISGNLSIMYGFQGPNIAL
                     VTACTTGTHSIGEAARIIEYGDADVMVAGGAESTVSPLGVGGFCAARALSGRNDDPQA
                     ASRPWDKDRDGFVLGEGAGVVVLEEYERAKKRGAKIYAEVSGYGRSADAYHMTQPAES
                     GEGAARCMGNALKNAGLNPDQIQYLNAHGTSTPLGDLAETMAVKRCFGDHAKRLVVNS
                     TKSMTGHLLGAAGGIEAVFTALAVHDQVSPPTINLDSPGEGCDLDYCAKTARPMKIDA
                     ALSNSFGFGGTNGTLLLRRV"
     gene            1538..1843
                     /locus_tag="VIS73_10800"
     CDS             1538..1843
                     /locus_tag="VIS73_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763683.1"
                     /translation="MFAAGVLLRASGKHAGVWLFDEDADGIGRLRLATGSVDDAPTFA
                     LRRRTCRPFLLVIEAGDVASGRSLRLGVLIDAVPREDFRRLAAWLRRARCAQGAGPE"
     gene            complement(1782..3404)
                     /gene="nadB"
                     /locus_tag="VIS73_10805"
     CDS             complement(1782..3404)
                     /gene="nadB"
                     /locus_tag="VIS73_10805"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEY5763684.1"
                     /translation="MGSKSHEFDVLIIGSGLAGQSAALRLADRFRVALVSKKSLSDSA
                     SAYAQGGIAAVLDDQDSIDAHIRDTLTAGAGLNNRRATRFVVENGKRAIDWLIEQGVP
                     FTRSGASYHLTREGGHSTRRVIHVNDATGSAVQKVLSEKIAQHPNITVLEDHIAIDLI
                     TGDRLHGATRRCYGAYLLRLADSTVLTVAAQHTVLATGGAGKAYLYTTNPVTATGDGI
                     AMAWRAGCRIANMEFIQFHPTCLYHPHVPSFLISEAVRGEGGILQLPDGTRFMPEHDP
                     RAELAPRDIVARAIDYEMKKRGLDFVHLDISHKEPGFVREHFPTIYARCLELGIDITK
                     DPIPVVPAAHYTCGGVMTDLDSRTDLPGLYAAGESAHTGLHGANRLASNSLLECLVYS
                     AAAAADIDRQPRVKMPALPEWDESRVTDADEEVVIAHNWDELRRFMWDYVGIVRTNKR
                     LRRALHRIRLLSHEIDEFYANFRVSNDLLELRNLVVNAELIVRCAMRRRESRGLHYSL
                     DFPEIADRARNTIIPGRRPVRNARVEATPPAA"
     gene            3561..4160
                     /gene="rpoE"
                     /locus_tag="VIS73_10810"
     CDS             3561..4160
                     /gene="rpoE"
                     /locus_tag="VIS73_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HEY5763685.1"
                     /translation="MTEREVDQLLVERAQQGDKKAFDELVTKYHKKLGRLLSRFVRDA
                     AEVEDVTQEAFIKAFRALPAFRGDSAFYTWLYRIGINTAKNYLVAQGRRAPTSTEYDA
                     NEAETFDDGDQLRDINTPESLLLSKQIGETVNVAMEGLPEELRTAIVLREIDGLSYEE
                     IAKMMQCPIGTVRSRIFRAREAIAGRLRPLLDTAPDKRW"
     gene            4172..4591
                     /locus_tag="VIS73_10815"
     CDS             4172..4591
                     /locus_tag="VIS73_10815"
                     /inference="COORDINATES: protein motif:HMM:NF016160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HEY5763686.1"
                     /translation="MSETLARAQVLRLNGTRAVIQAVDQGCGRCHQPGGCGGYRALTA
                     GRREYQVDNRFGAKVGDFVEVSVAEGTVARLAGLIYLLPLAGVIGGAVLGGDGVAGIA
                     TAIAGGLGGYLAARHWVRRENLVEPTVVRVCSNHQEK"
     gene            4594..6042
                     /locus_tag="VIS73_10820"
     CDS             4594..6042
                     /locus_tag="VIS73_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY5763687.1"
                     /translation="MKCLSLRFLRAPALALGIVGALTAGYVPAAAATGLPDFTLLVSQ
                     QGPSVVNISTTQTVKARSGPNLPFDENDPMYEFFRRFFPRQPGIPHDFEHKSLGSGFI
                     ISGDGYVMTNAHVVDQADEITVRLTDKREFSAKLIGADKRTDIALLKIDASGLPSVTL
                     GNPESLKVGEWVVAIGSPFGFENSVTAGIVSAKGRSLPQENYVPFIQTDVAVNPGNSG
                     GPLFNLKGEVVGINSQIYSRTGGFMGLSFAIPIDLAMEIQGQLRASGKVNRGRIGVVI
                     QEVTKELAESFGLTKPGGALVNAVEKGGPADKAGLEPGDVILKFDGKLVEQSGDLPRI
                     VGATRPGNKVVVQVWRKGAARDIKLTVGEMPEDRQVQGQPSKGSGNANKAVERLGIVV
                     SELSAAQRAELEIKGGVLVDEVKGGAARSDLRAGDIILAVIHRGSGFDVGSLEELNEA
                     LRKVDKSSNVTLLVRRGEVQTFVTVRGNGGGR"
     gene            6049..6297
                     /locus_tag="VIS73_10825"
     CDS             6049..6297
                     /locus_tag="VIS73_10825"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY5763688.1"
                     /translation="MAAGRLTLYGRGYCHLCDDMREALQPLLDEFGASLVEIDVDSDP
                     ALEDRYGELVPVLLGGEDELCHYFLDAPRVRAYLLEIR"
     gene            6355..>7300
                     /locus_tag="VIS73_10830"
     CDS             6355..>7300
                     /locus_tag="VIS73_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY5763689.1"
                     /translation="MRHIRNFSIIAHIDHGKSTLADRLIHRCGGLADREMEEQVLDSM
                     DIERERGITIKAQTAALRYQAADGQIYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIELGVEVLPVLNKIDLPSAEPDNARREIEEVIGIDASEAIL
                     ASAKTGLGVDEVLELVVRKVPPPVGDPEAPFKALIIDSWFDNYVGVIMLVRVIDGQLR
                     PKDRIVLMAEGSTHLCEQVGVFTPKSVAREQLSAGEVGFITAGIKDLTVAKVGDTVTR
                     LDHRAAKPLPGFKEIKPQVFAGLYPVEANQYDGLRDSLE"
BASE COUNT         1314 a         2176 c         2440 g         1370 t
ORIGIN      
        1 gccgagaaga tcacgacggt caagcaagct gtcgattacg tcgtcgcgca caagaagtaa
       61 cctactgttt tccgtatcgc ccggctgcgt cgtgcggccg gcgctccgtt tcaattctcc
      121 ctggagtaat ccgtgacacg ccgaagagtc gtcatcactg gcctgggggt cgtttctccg
      181 gtcggcaaca ccgtagcgga tgcctggaag aacatccttg caggcgtatc cggcattggc
      241 atgatctcgc gtttcgatac cacgaccttt tcgtcgcgca tcgccggcga agttcgtgat
      301 ttcgatattg gccagtatct gtcgccgaag gaagcacggc gcatggatcg cttcatccac
      361 tacggcatgg cggcgggcat ccaggcggtg cgcgacgccg gactcgaagc gagcccggcc
      421 gatgccgagc gcatcggtgt ggcaatcggt tcgggcatcg gcggcctgcc gatgatcgag
      481 gacaccaagg acgagtacat cgccggcggc gtccgcaagg tttcgccctt cttcgtgccc
      541 ggatcgatca tcaacatgat ttccggcaat ctctcgatca tgtacggctt ccagggtccg
      601 aatatcgcgc tggtcaccgc atgcaccacg ggtacccaca gcattggcga ggcagcgcgg
      661 atcattgaat acggcgatgc cgacgtgatg gttgccggtg gtgccgagtc gacggtgtcg
      721 ccgcttggtg tcggcggctt ctgcgccgcc cgcgcgctct cggggcgcaa cgacgacccg
      781 caggcggcca gccgtccatg ggacaaggac cgcgacggct tcgtgctcgg cgagggtgcc
      841 ggcgtcgtcg tgcttgagga gtacgagcgc gccaagaagc gcggcgcgaa gatttatgcc
      901 gaggtgagcg gctatggccg cagcgctgac gcctaccata tgacgcagcc cgccgagagc
      961 ggcgagggcg cggcgcggtg catgggcaat gcactgaaga acgcgggcct caacccggac
     1021 cagattcagt atctcaacgc ccacggcacg tcgacgccgc tcggtgacct tgccgaaacg
     1081 atggcggtca agcgctgctt cggcgatcac gccaaacggc tggtggtcaa ctcaacgaag
     1141 tcgatgaccg gacatctgct gggcgccgcc ggcggcatcg aggcggtgtt cacggcgctt
     1201 gccgttcacg accaggtttc accgccgacg atcaacctcg actcgccggg tgagggctgc
     1261 gacctcgatt actgcgcgaa gaccgcacga ccgatgaaaa tcgacgccgc cttgtcgaat
     1321 tccttcggtt tcggcgggac caacggcacg ctgctgctgc gccgcgtctg agccggaatg
     1381 gccgttgcta gcagttagcg gccagcttct ctgcggggca cgatcagccg ctcgcttctt
     1441 gcgctggcgt tgaccattct ggcgatcgcg cttgccgcga tcgccaccgc tcactcccgt
     1501 cttgccgatt cttgcgatgg cgttctgggc actgctggtg ttcgccgctg gcgtgctgtt
     1561 gcgcgcgtcg ggcaagcacg ccggtgtctg gctcttcgac gaagatgccg acggaatcgg
     1621 acgcctgcgc ctggcaacgg gaagcgtcga cgatgcgccg acattcgcgc ttcggcgccg
     1681 aacctgccgg ccgtttctgc tcgttatcga ggccggggac gtggcgagcg gccggtcgct
     1741 ccggctcggc gtgctgattg acgcggtgcc gcgcgaggat ttcaggcggc tggcggcgtg
     1801 gcttcgacgc gcgcgttgcg cacagggcgc cggcccggaa tgatggtgtt acgcgcccgg
     1861 tctgcgattt cgggaaagtc gaggctgtag tgcagcccgc ggctttcgcg gcgacgcatc
     1921 gcgcaacgga cgatcagttc tgcattgacg accagatttc gcagttcaag cagatcgttg
     1981 ctgacgcgga agtttgcgta gaactcgtcg atctcgtggg agagcaggcg aatgcgatgc
     2041 agcgcgcgcc gcaggcgctt gttggtgcgg acgatgccga cgtaatccca catgaagcgg
     2101 cgcaactcgt cccagttgtg ggcgattacc acctcctcgt cggcatcggt gacgcggctt
     2161 tcatcccatt cggggagcgc gggcatcttg actcgcggct ggcgatcgat gtcggctgcc
     2221 gctgccgcgg aatagaccag acattcgagc aatgaattgc tggccaaccg gttggcgccg
     2281 tgcagtccag tgtgcgcgga ttcgccagcg gcgtagagcc cgggcaggtc ggtccgtgaa
     2341 tcgagatcgg tcatgacgcc gccgcaggtg tagtgcgcag ccggaaccac ggggatcggg
     2401 tctttggtga tgtcgatccc gagttcgagg cagcgcgcat agatcgtcgg gaaatgctca
     2461 cggacgaagc ctggttcctt gtgcgagatg tcgaggtgaa cgaaatcaag gccgcgtttc
     2521 ttcatctcgt agtcaatcgc gcgggcgacg atgtcgcgcg gcgcgagttc ggcgcgcgga
     2581 tcgtgctcgg gcatgaagcg tgtcccgtcg ggcagctgga gaatcccccc ctcgccgcga
     2641 acggcctccg aaatcaggaa ggaagggaca tgcgggtgat agaggcatgt ggggtggaac
     2701 tggatgaatt ccatgttggc aatccggcag ccggcgcgcc aggccatcgc gatgccgtca
     2761 ccggtggcgg tgaccggatt ggtcgtgtag agatatgcct tgccggcgcc gccagtggcg
     2821 aggaccgtgt gttgggctgc gacagtcagc accgtgctgt cggcaagccg gagaagatag
     2881 gcaccgtaac agcgccgggt cgcgccgtgg agccggtcgc cggtgatcag gtcgatcgcg
     2941 atgtgatcct cgaggaccgt gatgttgggg tgctgcgcga tcttttccga aaggactttc
     3001 tgaaccgctg acccggttgc gtcgttgacg tggatgacac gccgcgtgct gtgtcctccc
     3061 tcgcgggtca gatggtagct tgcgccgctg cgcgtgaatg gcacgccctg ttcgatcagc
     3121 cagtcgatcg cgcgcttgcc gttttcgacg acgaagcgtg tggcgcggcg gttgttcaga
     3181 ccggcgccgg cggtcagcgt gtcgcggata tgtgcatcga tcgaatcctg atcgtcgagc
     3241 accgcggcga tgccgccctg cgcataggca ctggcgctat cgctaagcga tttcttgctg
     3301 accagcgcga cccggaaacg gtcggccagg cgcagcgcgg ccgattgccc ggcgaggccg
     3361 ctgccaatga ttaaaacgtc gaactcgtgc gactttgacc ccattgcggg cgccccgtct
     3421 tgaatgctga aggggtggcc ccatcaggtt ggacacgtgt cagggaatgc agaatctgcc
     3481 gaccaatatg cgcgtgtgac tacgttatac tgcctcgcat aactcgaaga accatacccg
     3541 ccgggaaacc agagatcaag atgaccgaac gcgaagtcga ccagcttctg gtcgagcgcg
     3601 cacaacaagg ggacaagaag gcgtttgacg agcttgtaac gaagtatcac aagaagctcg
     3661 ggcgtctgtt gtcacgcttt gttcgcgatg cggccgaagt ggaggatgtg acccaggagg
     3721 ccttcatcaa ggccttccgc gcgctgccgg cttttcgcgg cgacagcgcg ttctacactt
     3781 ggctctaccg tattggcatc aatacggcaa agaactatct cgttgcgcag ggccggcggg
     3841 cgccgacctc gaccgagtac gacgcgaacg aggccgaaac cttcgacgac ggcgaccaac
     3901 tgcgcgacat caacaccccc gagagcctgc tgctgtcaaa gcagatcggc gagaccgtga
     3961 acgtggcgat ggaagggctg cccgaggagt tgcggacggc gatcgtgctg cgcgaaatag
     4021 acggcctgag ttacgaggaa atcgccaaaa tgatgcagtg tcctattggc accgttcgtt
     4081 cgcggatttt ccgtgcgcga gaggcgattg ccggccgttt gcgtccactg ctggacaccg
     4141 cgcccgacaa gcgctggtag gacaggcagt cgtgtcggaa acgctcgctc gtgcgcaagt
     4201 tctgcgcctg aatggcacgc gtgccgtcat ccaggccgtt gaccaggggt gcgggcgctg
     4261 ccaccagccg ggtggttgcg gcggttaccg agcattgacc gccggccggc gcgaatatca
     4321 ggtcgacaat cggtttggcg ccaaagtcgg cgacttcgtc gaagtctcgg tagccgaagg
     4381 caccgttgcc cgtctcgccg gcctaatcta tctgctgcct ttggcgggcg tcattggcgg
     4441 ggccgttctg ggcggcgacg gcgtcgccgg catcgcgaca gccatcgccg gcggcctggg
     4501 cggctacctg gctgccaggc attgggtccg ccgcgaaaac cttgtcgagc cgactgttgt
     4561 cagagtctgc agtaatcatc aggagaaatg accatgaagt gtctttcgct gcgcttcctg
     4621 cgcgccccgg cgctagccct tggaatcgtc ggcgcgctga cggccggata cgtaccggcg
     4681 gcggcggcga ccggtttgcc ggatttcacc ctgctggtca gccagcaggg cccgtcggtc
     4741 gtcaatatca gcacgacgca gacggtcaag gcccgtagcg gtccgaacct gccgtttgat
     4801 gaaaacgatc cgatgtacga gttctttcgg cggtttttcc cgcgccagcc gggaattccg
     4861 cacgacttcg agcacaagtc gctgggttct ggcttcatca ttagcggcga cggttatgtg
     4921 atgaccaatg cgcatgtggt tgaccaagcc gatgagatta ccgtgcggct tacagacaaa
     4981 cgtgaattca gcgccaagct gatcggcgcc gacaagcgga ccgacattgc cctgcttaag
     5041 atcgacgcct cgggattgcc gagcgtgacc ctggggaatc ccgagtcgct caaggtcggc
     5101 gaatgggtcg tggcgatcgg ttcgcccttt ggcttcgaga actcggtcac cgccggaatt
     5161 gtcagcgcca agggacgcag cctgccgcaa gagaactacg ttccgttcat tcagaccgac
     5221 gtggcggtaa accccggtaa ttcgggcggg ccgctgttca acctgaaggg cgaggtggtt
     5281 ggcatcaaca gccaaatcta tagccgcacg ggcggtttca tgggtttgtc ctttgcgatt
     5341 ccaattgact tggccatgga aatccagggc cagctgcgtg ccagcggcaa ggtcaatcgc
     5401 ggccggatcg gggtggtcat ccaggaggta acgaaggagc ttgccgaatc atttggcctc
     5461 accaagccgg gaggtgcgct ggtcaacgcc gtcgagaagg gtgggcccgc cgacaaggcg
     5521 gggctggagc cgggcgacgt gattctcaag ttcgatggca agcttgtcga gcaatcgggg
     5581 gatctgccgc gaatcgtcgg cgcaacccgc cccggcaaca aggttgtggt gcaggtctgg
     5641 cgcaagggcg ctgcccgcga catcaagctg accgtcggcg agatgccaga ggatcgccag
     5701 gtgcagggtc agccgtccaa gggaagcggc aacgccaaca aggctgtcga gcgcctgggt
     5761 attgtcgtca gcgagttgag cgcggcacag cgtgcggagc tcgagatcaa gggcggagtc
     5821 ctagtcgacg aggtcaaggg aggcgctgct cgcagcgacc tgcgcgccgg cgacatcatt
     5881 ctcgcggtca tccaccgcgg aagcgggttc gacgtcggtt ccctcgaaga gctgaacgag
     5941 gcgctgcgca aggtcgacaa gagcagcaat gtcaccctgc tggtccggcg aggcgaagtg
     6001 cagaccttcg tcacggtgcg cggcaacggc ggcgggcgct gaggcggcat ggcagcaggc
     6061 cggctgacct tgtacggcag aggctattgc cacctctgtg acgatatgcg ggaggccctg
     6121 cagccgctgc tcgacgagtt cggtgccagc ttggtcgaga tcgacgttga cagcgatccg
     6181 gcacttgaag accgctacgg cgagttggta ccggtattgc tggggggcga ggacgagctc
     6241 tgtcattact tcctcgatgc gccccgcgtc cgtgcgtatc tgcttgaaat tcgatagaat
     6301 cgcgcccgca caacaacgct tagagtgagg gcgctacagc gccccttttt tttgatgcgt
     6361 cacatccgca acttctcaat catcgcgcat atcgaccacg gcaaatcgac gctggccgat
     6421 cgtctcatcc accgctgtgg gggtcttgca gaccgcgaga tggaggagca ggtccttgat
     6481 tcgatggata tcgagcgcga gcgcggcatt accatcaagg cgcagactgc ggcgctccga
     6541 tatcaggcgg cagatggcca gatctaccag ctcaatctca tcgacacgcc gggacacgtt
     6601 gatttctctt acgaagtctc gcgttcgctg tcggcctgcg agggcgcgct gctggtggta
     6661 gacgcctcgc agggcgtcga ggcgcagacc gttgccaact gctatacggc gattgaactc
     6721 ggcgtcgagg tcttgccggt cctcaacaag atcgatctgc catcggccga gcccgacaac
     6781 gcgcggcgtg aaatcgagga ggtcatcggc atcgatgcct ctgaggcgat tctggcgtca
     6841 gcgaagacgg gcctgggcgt cgacgaggtg ctcgaactgg tggtgcgcaa ggttccaccg
     6901 ccggtgggtg accccgaggc gccgttcaag gcgctgatca tcgactcatg gttcgacaac
     6961 tacgtcggcg tcatcatgct cgtccgcgtg atcgacggcc agttgcgccc gaaggacagg
     7021 atcgtcctga tggccgaggg cagtacccac ctgtgcgaac aggtcggcgt gtttacgccc
     7081 aagtcggttg cccgcgagca actgtcggcc ggcgaggtcg gcttcattac cgccggcatc
     7141 aaggacctga ccgtggccaa ggtcggcgac acggtgacgc gccttgacca tcgtgctgct
     7201 aagccgctgc cgggtttcaa ggagatcaag ccgcaggttt tcgccggtct gtatccggtc
     7261 gaggccaatc agtatgacgg gctccgcgat tcgctcgaga
//
LOCUS       DATDAA010000361         2817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_60748,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000361 DATDAA010000000
VERSION     DATDAA010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60748"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..2033)
                     /locus_tag="VIS73_10835"
     CDS             complement(120..2033)
                     /locus_tag="VIS73_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763690.1"
                     /translation="MSLRDRRRVAIAAIVGAALAMLPSFGQAMAPTVNGHLRLVQPAS
                     EVVAADAARTIVIDAGAYRGPLILIVDGTDVSARVRREGERLVYELSAPLNEGVHRVQ
                     VIAPDPSGPGNAEWTFTVGAGVAASGRREVYSRSSLSASASRVFGDPVQGDDWVSNAS
                     LGIDVGVRSGETELSLQTQLAYASTADNNDFEPNGYVLTLRNDDDSLALGDVSFRGTP
                     LTAPGLARRGALAQLNFAGTAIQIAQVNSGTVTGWRDGLGSDNQIRAASLSQPITRSG
                     DDPFTLTAIVVRGESANAGSSNVATVEPPTSGRVAGLQGSGRLFGTGLNFEGAWSDFD
                     ADTTDGVAARRDQAVVLQLDGALGPIGLSGSYQYAGPDFSSIANPGANRDREQFGLSA
                     GTAAGIHTFSATVSRARDNVDDDLTRPVIVNDSAGLTWAMNPSDWPTLSLSYQAGRVE
                     SEKEPPATTPTRSTNDALSAAASLAREHWAANLALSRSGVADAANGDSDATSAQFSVT
                     LHPTQDLNLAPTVAYTRSEAAAVTRETRLATLTLGWRVAEVWTLDGQLGHSADEADDG
                     STDSVRQNGTLRLAWSGSWLKSLFPGQQASVSLAASWSDLEDRVAPANNTSSSSVLLG
                     INVAFPLSGSYGF"
BASE COUNT          501 a          934 c          877 g          505 t
ORIGIN      
        1 ccgcgccctg cgccggcagc gcaccggacg cgcccgggat caccgggcgc atatcagcgc
       61 aggcgccgag cgcgccggcc accgccagaa tcgccagcca atgcctcggg ttgagtcgct
      121 tagaacccat agcttccgct cagtggaaag gcgacattga tgccaagcag cacgctgctg
      181 ctcgaggtgt tgtttgccgg cgcaacccgg tcttccagat ctgaccacga ggccgcaagc
      241 gacacgcttg cctgctgccc gggaaacagt gacttgagcc agctgccgga ccaggcgagg
      301 cgcagcgtgc cgttctggcg aacgctgtcg gtgctgccgt catcggcttc gtcggccgaa
      361 tggccgagtt ggccatccaa ggtccagacc tcggcgacgc gccaaccgag cgtcaaggtc
      421 gccagccttg tctcgcgcgt caccgcggcg gcttcgctgc gcgtgtaggc gacagtcggc
      481 gccaggttca ggtcctgagt ggggtgcagg gtcactgaaa attgcgccga tgtcgcgtcg
      541 ctgtcgccgt tcgccgcgtc cgcaacccca gaccggctga gcgcgagatt ggccgcccag
      601 tgctcgcgag ctagcgacgc cgccgcagag agtgcgtcgt tggtactgcg tgtcggcgtg
      661 gtcgccggcg gctccttctc gctctcgacg cgcccggcct gataggaaag cgagagcgtt
      721 ggccagtcac ttgggttcat ggcccaggtc aatccggcgc tgtcattgac gatcaccggc
      781 cgcgtcaggt catcgtcgac gttgtcgcgc gctcgcgaaa ccgtcgccga gaatgtatga
      841 atgccggcgg cggtgcccgc cgatagtccg aactgttccc ggtcgcggtt ggcgccggga
      901 ttggcaatgc tcgaaaagtc cggcccggcg tattgataac taccacttag cccgatgggc
      961 ccgagcgcgc cgtctagctg caacaccacc gcctgatcgc gccgcgcagc gacaccatcg
     1021 gtggtatcgg cgtcaaaatc actccaggca ccctcgaagt tcaatcccgt accgaacagt
     1081 cgaccggaac cctgcaggcc ggcaaccctg ccgctggtcg gcggttccac ggtcgcgacg
     1141 ttgctcgagc cggcattggc gctttcgcct ctgaccacaa tcgcggtcag ggtgaacggg
     1201 tcgtcgcctg agcgcgtgat cggctgcgat agcgaggccg cgcggatctg gttgtcggaa
     1261 ccgagtccgt cgcgccagcc ggttaccgtg ccgctgttga cctgggcgat ctggattgcc
     1321 gtgccggcga agttgagctg cgccagcgcg ccgcggcgcg ccaggccggg cgctgtcagt
     1381 ggggtgccgc ggaacgagac atcgccgagc gcgagcgagt cgtcgtcgtt ccgcaaggtc
     1441 aggacgtagc cgtttggctc gaagtcgttg ttatccgccg tgctcgcgta cgcgagctgg
     1501 gtctgcagcg agagttcggt ttcgcccgag cgcacgccta catcgatgcc gaggctggca
     1561 ttgctgaccc agtcgtcgcc ctgcaccgga tccccgaaca cgcggctggc ggaggcagac
     1621 aggctgctgc gcgaatacac ctcccgcctt ccgcttgcag cgactccggc gccaacggtg
     1681 aatgtccatt cggcgttgcc tggccccgaa gggtcgggag caatcacctg tacccggtga
     1741 acgccttcat tgagcggtgc cgacagctcg tataccagtc gctcaccctc gcgccgcaca
     1801 cgcgcactga catcggtgcc atcgacgatc aggatcagcg gcccccgata ggcgccggca
     1861 tcgatgacga tggttcgagc cgcatccgcg gcgacgacct cagaagccgg ctggacgagc
     1921 cgcagatgcc cgttgaccgt cggcgccatt gcctgcccga aggacggcag cattgcgagc
     1981 gcggccccaa cgattgctgc gatcgcgacg cgcctccggt cccgaagact catggcgtcg
     2041 ccagaacgcg tgggggaaaa tcaagcatgc tggtgcgcac tcgccggtgt gagtctgcga
     2101 acgaggcaat gcgatctcgc tgccttcggc gatttctgcg taacgcccca ctccgggtgg
     2161 gcagctgccg agttgaccta atcgcaacgc ccatttccgc ctccccggat aaaggcttat
     2221 ggatcgatag gtcgcctgcg catgccgtcg ggtccaactg ggctgtgagt tccagcgcgc
     2281 gcctgcaccg tccagcggcg tccgtcggtc gaatcccgat tcccccttag ggaatctccg
     2341 tcactcgccg gtaccgttgg atttggcgcg cccgcccttc ggttcctccg ctaaccacat
     2401 gaaattcctc aattacttcc aaccgcacgg taaattatcg cgacggtagg tgcgcaccga
     2461 atagaaggat aggctaccca attaacatct tccagaaata tttttgctta gatgacttgc
     2521 accatctcaa taacggaatg cacgaaatac caacgacgtc agcaagatgg tgttgcgtgt
     2581 cgcgcttggg cttgactgct cgaaaccagc tcttcagtca aaggagatgc cggacaacgg
     2641 cacaccgacg gaaagcggca gtcaactcga tccaagccaa acgtccgaat aggcgtgggt
     2701 acctgcgtag gcagtcggtc aacccttcga gcgaagccga cgctcggatc tccattccta
     2761 gacagaccta ccggccggtc acggccgatt ttttccttca ccctcaccaa actgaag
//
LOCUS       DATDAA010000362         2152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_60796,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000362 DATDAA010000000
VERSION     DATDAA010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60796"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VIS73_10840"
     CDS             <1..715
                     /locus_tag="VIS73_10840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY5763691.1"
                     /translation="LGYVLEAEFFERSPGLQTLSIDRYPPTPCYDWRQIHKLFEMPAL
                     NRDMKDVTQPPATVELLRLAKAGDRGATNALFERLFPPLLRFARGRLPGWARGMIDTT
                     DLVQETLLSAYGVIGKDQAIGDGAIHAYLRATLKNRLIDELRKVERRPRLDEMDKTQR
                     GNSATPLEELIGIEALERYEDALGKIPRQDRDAVLARIELGLSYAEIARMLDRPSADA
                     ARMSVSRAMLRLARAMSHE"
     gene            708..>2152
                     /locus_tag="VIS73_10845"
     CDS             708..>2152
                     /locus_tag="VIS73_10845"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5763692.1"
                     /translation="MSDALTQIAASVSDGEDIDWQSLLETTDEPATLANLKIVALLAD
                     SLKRLDEPPAPASWGGLDVRSRIDGGAYADVYKAYDAALDREVALKLYRHAEGPRSER
                     LLAEGKLLARVDHPNVVRVHGAAQHDGVTGLWMECIDGESLQDRIIAGQTPSGREAAV
                     TGLDIARGLAGIHAAGVIHGDIKAANVLRDDKGRIRIADFGSGFDIESAEQAIRVSGT
                     PLYLAPEVLDGQPPSVASDIYSLGVLMFFLLSGRYPREAGSIDELRAAPADARHHLRD
                     LRPDLDDELQRIVERCLADDPGSRPRSAGEAAEALSAYLGGAAPQRAVFDKRQWIAAA
                     VATVVLISLILMRSPGYSLDVELLVSADGRPAVSVADGGVVGLGDSLQLALASDKPLY
                     VYVFSESGSGESWGLFPLPALGLDNPVAAADARRLPGDGWSWLVSHPAATETIHLVAS
                     PSPVAELESAYAGLPPAEQSGAFASRGIGRVV"
BASE COUNT          360 a          696 c          747 g          349 t
ORIGIN      
        1 cctcggatac gtcttggagg ctgagttctt tgaaaggagc ccgggtttgc agacattatc
       61 aatagaccgc tatccgccga cgccttgcta tgattggcgg cagatccaca agctcttcga
      121 gatgcctgcg ttgaaccggg acatgaaaga cgtgacgcaa ccgccggcaa cggtagagtt
      181 gctgcggctg gccaaggccg gggacagggg cgctaccaat gcgctcttcg aacgcttgtt
      241 cccgcccctg ctgcgcttcg cccgcggccg cctgccgggt tgggcgcgcg gcatgatcga
      301 caccaccgac ctggtccagg aaacgctgct ttccgcctac ggcgtgatcg ggaaagacca
      361 ggcgattggc gatggcgcga ttcacgcgta cctgcgcgcg actttgaaaa accgcctgat
      421 cgacgaactg cgcaaggtgg aacgccgtcc caggctcgat gaaatggaca agacgcagcg
      481 cggaaattcg gcaacgccgc tcgaggagct tatcggcatc gaggcgctgg agcgctacga
      541 agatgcgctc ggtaaaatcc cccggcagga ccgcgatgcc gtgctggcgc ggatcgaact
      601 cggcctgtcc tatgccgaga tcgcccgcat gctcgaccgt ccctcggccg acgcggcgcg
      661 catgtcggtg agccgcgcaa tgctccgcct ggcgagggcg atgagtcatg agtgatgcgc
      721 tgacgcaaat cgcggcgagc gtctccgacg gcgaggacat cgactggcag tcgctgctcg
      781 aaaccacgga cgagcccgcg acgctggcca atctgaaaat cgtcgcgctg ctggccgaca
      841 gcctgaaacg actggacgag ccgccggcgc cggcaagctg gggcgggctg gacgtgcgtt
      901 cgcgaatcga cggcggcgct tacgccgacg tctacaaggc ctacgatgcg gccctcgatc
      961 gtgaggtcgc cctgaaactt taccggcatg ccgaggggcc gcgctcggaa cgcctgttgg
     1021 ccgagggcaa gctgctcgcc agggtcgatc acccgaacgt cgtgcgtgta cacggggccg
     1081 cgcaacacga cggcgtgacc ggtctgtgga tggaatgcat cgacggcgag tcgctgcagg
     1141 acaggatcat cgcggggcag acgccgagcg gccgcgaggc ggcggtgacg ggactcgata
     1201 tcgcccgcgg gctggccggt atacatgccg ctggcgtgat ccacggcgat atcaaggccg
     1261 ccaacgtgct gcgcgacgac aagggcagga tccgcattgc ggatttcggg tcgggtttcg
     1321 acatcgaaag cgccgaacag gcgattcgcg tcagcggcac gccgctctac cttgcgccgg
     1381 aagttctgga cggtcagccg ccgtccgtcg catccgatat atacagctta ggcgtgctga
     1441 tgttcttcct tttgagcggt cgctacccgc gggaggccgg gtccatcgac gagctgcggg
     1501 cagcgccggc ggacgcccgg catcacctgc gcgacctgcg ccccgacctt gacgacgaac
     1561 tgcagcgaat cgtcgaacgc tgcctggccg acgatccggg gtcgcggccg cgcagcgcgg
     1621 gggaagccgc cgaggccttg tccgcctacc tcggcggggc ggcgccacaa cgagccgtgt
     1681 tcgacaagcg tcagtggatc gcggcggcgg tcgccaccgt cgtgctgatt tctctcatcc
     1741 tgatgcgttc gccgggttat tcgctggacg tcgagctgct ggtatcggcc gacggccggc
     1801 cggcggtgag cgtggcggac ggcggcgtcg tcggcctggg cgacagcctg cagctggcgc
     1861 tggcctcgga caagcctctc tatgtttacg tattcagcga gagcggcagc ggcgaaagct
     1921 ggggcctgtt tcccctgccg gcgctgggcc tcgacaaccc cgtggcagcg gccgatgccc
     1981 gcaggcttcc aggggacggc tggtcctggc tcgtcagcca cccggcggcg acggaaacca
     2041 ttcatctcgt cgccagcccg tcgcctgtcg cggaactgga atcggcctac gcgggcctgc
     2101 cgccggcgga gcagtcaggg gccttcgcct cccgcgggat cgggcgggtg gt
//
LOCUS       DATDAA010000363         2899 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_201474,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000363 DATDAA010000000
VERSION     DATDAA010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201474"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VIS73_10850"
     CDS             complement(<1..227)
                     /locus_tag="VIS73_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5763693.1"
                     /translation="MTDHSGFFDAVRTAGISPSDSEDIRLQKSLLMFATGLVSVASML
                     WLFLYWQMGPQLSATIPFAFQLLLAGNMAFYL"
     gene            423..1391
                     /gene="ispB"
                     /locus_tag="VIS73_10855"
     CDS             423..1391
                     /gene="ispB"
                     /locus_tag="VIS73_10855"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEY5763694.1"
                     /translation="MPQDRLYALIAPDMSAVDAVIRDRLHSEVELVRDVAEYIVRSGG
                     KRMRPALVLMAAKASGYQGAAHHELAAVVEFIHTATLLHDDVVDGSDLRRGRQTANAV
                     FGNAASVLVGDFLYSRAFQMMVSVGSMRVMEVLSEATNVIAEGEVLQLMNCHDADVDV
                     DRYLKVIRYKTAKLFEASARIGGVLGGVSPEVEEGLAQFGIHLGTAFQLVDDVLDYSG
                     DEVDTGKHVGDDLAEGKPTLPLIYAMQRGTGEQSATIRQAIEVGGRDAMPAVMDAVRA
                     TGALAFTQRQAELEIDAANTALAVLPPSQYKDSLLEFAAFSVSRRS"
     gene            1405..1481
                     /locus_tag="VIS73_10860"
     tRNA            1405..1481
                     /locus_tag="VIS73_10860"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1439..1441,aa:Pro,seq:cgg)
     gene            1562..1786
                     /locus_tag="VIS73_10865"
     CDS             1562..1786
                     /locus_tag="VIS73_10865"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HEY5763695.1"
                     /translation="MGRLLLLIALAVLALFIWRKLTAGPALGRRDAPQVKEEEIVACA
                     HCGLHVPASEALREDDDVYCCSEHLRRGRR"
     gene            1737..>2899
                     /locus_tag="VIS73_10870"
     CDS             1737..>2899
                     /locus_tag="VIS73_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY5763696.1"
                     /translation="MTTSTAVQSTSGEDDVNHADSRARGNREAASPSVAMPWRPLVYL
                     SVYRLLLATLLLAGALSAIDSFSLGFASGSTRRIFSGLLGVYWAICLASLVGVWRFRR
                     SFDFQLTVQVVVDVLLFSALIFLSGGLRSGFGVMLLVTLAGASLVGQGRLTLFYAALA
                     TIGVLGQEVIQSVSGDFEPSDFFLAGILCLGFFGTAGTVRVLARRLIANEELARRRGI
                     ALRRQMEVSDTVIALMQDGILVVTASGLIQQANPRARILIGLDEPGDDSVAEISPALA
                     ADFAAWLAGDQAVSPCDFRARGSGLQLQARFVRVAGEGDDTLIFIDDAGRARAEAQKV
                     KLAALGRLTASIAHEIRNPLSAIRHAGELLGETLGNEQDQRLIRIVADNTKRL"
BASE COUNT          473 a          886 c          972 g          568 t
ORIGIN      
        1 aggtagaagg ccatattgcc ggccagcagc aattggaagg caaagggaat ggtggccgaa
       61 agctgcggcc ccatctgcca gtaaagaaac agccagagca tcgacgcgac gctgaccaag
      121 cccgtggcga acatgagcag cgacttctgc aggcggatat cttcgctgtc gctcggcgag
      181 atgccggcgg tgcggacggc gtcaaagaat ccggaatggt cggtcactga ggtctcggca
      241 gggggtgcgc ggcgggggcg gagcctatct tcgcaatttg gactcggttc ggtcaatggc
      301 tggatttgac cgtggcatga atcgccgcgc cgcctcgggg tgcgccagcc gccgatgaac
      361 gtggcgcgag atcggcgatc ccgctatatt tgcgcctccg tttttccgct ggtctgtcat
      421 ttgtgccaca agatcgcctt tatgccctga tcgccccgga catgagcgcc gtcgacgcgg
      481 tcatccgcga tcgtctgcac tccgaagtcg agctggtccg cgatgtagcc gaatacatcg
      541 tccgcagtgg cggcaagcgt atgaggccgg cgctggtgtt gatggcagcc aaagccagcg
      601 gctatcaggg agcggcccac catgaactgg cggcggtagt cgagttcatt cataccgcga
      661 ccctgctcca cgacgatgtg gtcgacgggt ccgacctgcg gcgtggccgg cagactgcca
      721 atgccgtgtt cggcaatgcc gctagcgtgc tggttggcga tttcctgtat tcgcgcgcct
      781 tccagatgat ggtgtcggtc gggtcgatgc gggtgatgga ggtgctgtcc gaggcgacca
      841 acgtcattgc cgagggcgag gtccttcagt tgatgaactg ccacgacgcc gatgtcgacg
      901 tcgatcgcta tctcaaggtc atccgctaca agacggccaa gctgttcgag gcttcggcaa
      961 ggataggtgg cgttctgggt ggagtgtcgc cggaggtcga ggaaggcctg gcgcagttcg
     1021 gcattcactt gggaaccgcg tttcagctgg tcgacgacgt gctcgactat tcaggagacg
     1081 aggtcgatac cggcaagcac gtcggcgacg atctggccga aggcaagccg acgctgccgc
     1141 tgatctacgc gatgcagcgg ggcactggcg agcagtcggc gacgattcgt caggcgatcg
     1201 aggtcggcgg gcgcgatgcc atgccggccg tcatggatgc cgtacgggcg accggggcgc
     1261 tggcgtttac ccagcgccag gccgaactcg aaatcgatgc agcgaacacg gctttggccg
     1321 ttcttccgcc ttcccaatac aaggattcgt tgctagaatt cgccgccttc tcggtgtcac
     1381 gtcgcagcta gcgtgaaacc tcgtcggagt gtagctcagc ctggtagagc actgcgttcg
     1441 ggacgcaggg gccggaggtt cgaatcctct cactccgacc atttgacgat ggccctcttc
     1501 gattcgactg gtctcgcccg gcctgctggc cgttccggcg ggcatcggca ataatccacg
     1561 catgggccgc ttacttctcc tgattgcctt ggccgttctg gcgctgttca tctggcgcaa
     1621 gctgacggct ggcccggcac tcggtcggcg cgacgcgccg caagtcaagg aagaagaaat
     1681 cgtcgcctgc gcgcattgcg gcctgcacgt gccggcgtcc gaggcgcttc gcgaagatga
     1741 cgacgtctac tgctgttcag agcacctccg gcgaggacga cgttaaccac gccgacagtc
     1801 gcgcccgcgg gaaccgcgag gcggcatccc cttcggtggc gatgccctgg cggccgctgg
     1861 tttacctcag tgtttatcga ctgctgctgg cgacgctgct gctcgccggc gcgctgagcg
     1921 ccatcgattc gttctcgctc ggattcgcgt caggaagtac caggcgaatc ttcagcggtc
     1981 tactcggagt ctattgggcg atttgcctgg cgtcgctggt cggcgtctgg cgctttcgac
     2041 ggtccttcga tttccagctg acggttcagg tagtcgtcga tgtcctgctc ttcagcgcct
     2101 tgatctttct cagcgggggg ctgcgctcgg gtttcggcgt catgctactg gtcacgctgg
     2161 ccggcgccag tctggtcggg cagggccgac tgacgctgtt ctacgcggcc ttggcgacca
     2221 tcggcgtgct cgggcaggag gtcatccaga gtgtcagcgg tgacttcgag ccctcggatt
     2281 tctttctcgc cggcatactc tgcctcgggt tcttcggaac cgccgggacc gtccgcgtgc
     2341 ttgcccgtcg gctgatcgcc aacgaggagt tggcgcgtcg acgcggcatc gcactacgtc
     2401 ggcagatgga ggtcagcgac acggtgatcg ccctgatgca ggacggcatt ctggtggtga
     2461 cggcatccgg cctgatccag caggccaatc cgcgggcccg catactgatc ggtctcgacg
     2521 agcccggcga cgacagcgtc gccgagattt cgccggcatt ggctgccgat ttcgcggcgt
     2581 ggctcgctgg cgatcaagcg gtatcgccct gcgactttcg cgcccgcggc agcggcctcc
     2641 agttgcaggc acgttttgtc cgcgtcgcgg gcgaaggcga cgacacgctg atctttatcg
     2701 atgatgccgg gcgtgcccgc gccgaggcgc agaaggtcaa gctcgcggcg ctcggcaggc
     2761 tgacagcgag catcgcccac gagatccgca acccgctgtc ggcgattcgc cacgccgggg
     2821 agctgctcgg cgagacgctg ggcaacgagc aggatcagcg cctgattcgc atcgtcgccg
     2881 acaacacgaa acgcctgga
//
LOCUS       DATDAA010000364         2504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_376800,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000364 DATDAA010000000
VERSION     DATDAA010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376800"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..2086
                     /gene="ilvD"
                     /locus_tag="VIS73_10875"
     CDS             230..2086
                     /gene="ilvD"
                     /locus_tag="VIS73_10875"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY5763697.1"
                     /translation="MPVYRSKTSTHGRNMAGARALWRATGVKEGDFGKPIIAVVNSFT
                     QFVPGHVHLKDLGQLVAREIEVAGGIAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPAIFVSGGPMEAGKVKWQGKII
                     AVDLVDAMVKAGDEKVSDAEVDAFERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GTIVATHAARQNLFLEAGRRIVGLAKRYYEHDDATVLPRGIATKAAFENAMALDVSMG
                     GSTNTVLHMLAAAQEGGVDFTMADIDRISRKVPCLCKVAPAISTVHIEDVHRAGGIFG
                     ILGELDRAGLIQREVPTVHAPSMGEAIDFWDVKRNTTKAVHRWYSAAPGGVPTQVAFS
                     QDRAYPTLDLDREAGVIREKAHAFSQDGGLAVLHGNIAEDGCIVKTAGVDESIWNFTG
                     RARVFESQEAAVEAILGHKVVAGDVVVIRYEGPMGGPGMQEMLYPTSYLKSRGLGKEC
                     ALLTDGRFSGGTSGLSIGHASPEAAAGGAIGLIEEGDAIVIDIPHRRIHLAVAEEELT
                     RRRAAMEAKGADAWKPVSRERVVSQALQAYALMTTSADRGAVRDLGRLTAKS"
     gene            complement(2028..2489)
                     /locus_tag="VIS73_10880"
     CDS             complement(2028..2489)
                     /locus_tag="VIS73_10880"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY5763698.1"
                     /translation="MGCELAQIAKSIVFRATASDRAVVVVTSGALRVDEQQVAAVIGE
                     PVGKAGADFVRSRTGFAIGGVGTGSRARGEGEDLGRDSRRLKRTHDTRRRRGGVERGC
                     VDDANYRTTPLSLRNILRAGCVAPPPGGRAAALTTWRSADRDRARHRGQPR"
BASE COUNT          431 a          850 c          818 g          405 t
ORIGIN      
        1 attcttttct gcctagcgga gtctcagcta gctgaagatt atccccgatc gctggcgcct
       61 cggtgccgcc gggtttggcg aatgctccac catcactgcc ggagacgccg tcactgctag
      121 aatcgacgct cccgtcgaac ccgacgcaag aagctgcccc gtgccggacc cggcacgaat
      181 cgctaccccc gcgccccaag gcgcaatagt ttgccgcagg agaccccgca tgcccgtcta
      241 tcgttcgaag acttccaccc acggccgcaa catggctggc gcgcgcgccc tctggcgcgc
      301 gaccggcgtg aaggaaggcg acttcggcaa gccgatcatc gcggtggtca attccttcac
      361 ccagttcgtt ccgggccacg tgcacctgaa ggatctcggc caactcgtcg cccgcgagat
      421 cgaggtggcc ggcggcatcg ccaaggagtt caacacgatc gccgtcgacg acggaatcgc
      481 catgggtcac ggtggcatgc tctattcgct gccgagccgc gagttgatcg ccgactcggt
      541 ggagtacatg gtaaacgcgc actgtgccga cgccatggtc tgcatctcca actgcgacaa
      601 gatcacgccg gggatgctga tggcggcgat gcgcctcaac attcccgcga tcttcgtctc
      661 gggcgggccg atggaggccg gcaaggtcaa atggcagggc aagatcatcg ccgtcgatct
      721 ggtcgatgcc atggtcaagg ccggcgatga aaaggtctcc gacgccgagg tcgacgcctt
      781 cgaacgctcg gcgtgcccga cctgtggctc gtgttcaggg atgttcacgg ccaactcgat
      841 gaactgcctg accgaggcgc tcggtttgtc gctgcccggc aacggcacca tcgtcgccac
      901 ccacgccgcc cgccagaacc tgttcctcga ggccggccgg cgcattgtcg ggttggccaa
      961 gcgttattac gagcacgatg acgccacggt gctgccgcgc ggcatcgcca ccaaggctgc
     1021 cttcgagaac gcgatggcgc tcgacgtgtc gatgggcggg tcgaccaaca ccgtgctgca
     1081 catgctcgcc gccgcgcagg aaggcggcgt cgacttcacg atggccgaca ttgaccggat
     1141 ttcacggaaa gtcccttgcc tgtgcaaggt tgccccggcg atctcgaccg tgcacatcga
     1201 ggacgtgcac cgtgccggcg gcatcttcgg catcctcggc gaactcgacc gcgccgggct
     1261 gatccagcgt gaggtgccga ccgtgcacgc gccgagcatg ggtgaggcga tcgatttctg
     1321 ggacgtcaag cgcaacacca ccaaggccgt gcaccgctgg tatagcgccg cgccaggtgg
     1381 cgtgccgacg caggtcgcct tctcgcagga ccgagcctat ccgacactcg acctcgaccg
     1441 cgaagccggg gtcatccgcg aaaaggccca cgccttttcg caggacggcg ggctcgccgt
     1501 gctccacggc aacattgccg aggacggctg catcgtcaag acggccggcg tcgacgagtc
     1561 gatctggaac ttcaccggcc gcgcccgagt gttcgagagc caggaagccg cggtcgaggc
     1621 catcctcggc cacaaggtcg ttgctggcga cgtggttgtc atccgctacg aaggaccgat
     1681 gggcggcccc ggcatgcagg aaatgcttta tccgacgagt tacctcaaat cgcgcgggct
     1741 tggcaaggaa tgcgcgttgc tcaccgacgg ccgcttctcg ggcggcacct cgggcctgtc
     1801 gatcggccac gcctcgccgg aagcggcggc tggaggcgcg atcggtctga tcgaggaagg
     1861 cgacgcgatc gtcattgaca tcccgcaccg gcgaatccac cttgcagtgg ccgaagaaga
     1921 actgacgcgt cgtcgcgcgg cgatggaggc gaaaggcgcc gacgcctgga agccggtcag
     1981 ccgcgaacgc gtcgtctcac aggcgctgca ggcctatgca ctgatgacta cctcggctga
     2041 ccgcggtgcc gtgcgcgatc tcggtcggct gaccgccaag tcgtgagcgc cgccgcccgg
     2101 ccacccggag ggggcgcgac acatcccgcg cggagaatgt tgcgtagcga tagcggggta
     2161 gtccggtaat tcgcgtcgtc gacacagcca cgctcgacgc cgcctcttcg acggcgcgtg
     2221 tcgtgcgtgc gcttgaggcg gcgggagtcg cgaccgagat cctcgccttc gccgcgggca
     2281 cgcgaaccag tgccgacgcc gccgatcgcg aagccggtcc tgctgcgcac gaagtcggcg
     2341 ccggccttgc cgaccggctc gccgatcacc gcggcgactt gctgctcgtc gacgcgcagc
     2401 gcgccgctgg taacgacgac gacggcccga tcactggctg tggcgcgaaa gacgatcgac
     2461 ttggcgatct gcgccagttc gcaacctata gccgctgcgg cgtc
//
LOCUS       DATDAA010000365         3358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_515862,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000365 DATDAA010000000
VERSION     DATDAA010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515862"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VIS73_10885"
     CDS             <1..436
                     /locus_tag="VIS73_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY5763699.1"
                     /translation="VMRRVVEAGVPLFQLGNRAYCEEEREARRQYKVHAIDADQLVPQ
                     GIHSITLPADFPPQVFFTLDVDGIDPAVLPATGTPVPGGLSWYQTLGLFESVARQRRI
                     VGFDILEFAPIAGFHAYDFAAALLAYKLMGIVQRTAGAGSEP"
     gene            707..1810
                     /gene="ydiK"
                     /locus_tag="VIS73_10890"
     CDS             707..1810
                     /gene="ydiK"
                     /locus_tag="VIS73_10890"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HEY5763700.1"
                     /translation="MQRDLVRTILEVLVIAALVVASLWILSPFLASIVWATMIVVATW
                     PLFLALEARLWGRRGLAVTVMTLAILLVLFVPLSLAIGALVQNADAAVAFGKRIAQEG
                     LPPPPRSVETLPLVGEYLAKAWRAVLELGLADLWKRIAPYLGEVAKWIAARAGGLGLV
                     AVHLLLTAVISAILYVNGEAAARMAHRFATRLAGKAGEASVVLAGQAIRAVAIGIVVT
                     ALIQSVVGGIGLLVAGVPHVGMLAALMFMFCIAQLGPGFVLFPAVAWMYWQGVHGWAT
                     FLLVWSVGVVLMDNFLRPILIRRGADLPLLLIFAGVIGGLLAMGLLGLFVGPVVLAVT
                     YRLTEAWVDEALPQAAPATPTAQRANTSNDEPT"
     gene            complement(1774..>3358)
                     /locus_tag="VIS73_10895"
     CDS             complement(1774..>3358)
                     /locus_tag="VIS73_10895"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY5763701.1"
                     /translation="MFVRNLPVDVARKACGDVVLAVTVGNPPPTRGDLKDALSVLGRA
                     ASLGTELNERAQLATLGKQDVAVKVVLDGIGATSFDRIPEAIPIGEAAARKLAARLGR
                     YSLSRAQYAAWRAGLGDVSVARVERIDEVRIEGTEDVNPEVLRHFIAMKPGDAFDPEK
                     ASADTTRLVGRGDFENVDFHMQREDGRNVLTYTVREKNLGTNQLLFDLNMQTDFQGDT
                     RWGIRVDHRRSWVNRLGGEWRNTLQVGQPALLASEFYQPIDEAQRFFVAPRVYFSSSF
                     EDLFLDEQRVARLEVERYGIAADVGIAFDTWGEWRFGLDRGRADIDVDIGNPLAVSGQ
                     RIAIGQWQTRLEYDQLDNAVFPTRGGRGLIRYYESRKSLGADDEYRKGRLYWRSVVSY
                     GRNVVEFAVRGGTDFGSATPVYDQFALGGLNNFSGYQIGALRGREYLLGTAGFRRRIF
                     DLTGTLGTGVYLGGGVEAGNVFKRLDRTPASGVIWSGSAYLIIDSTLGPLYAGAGYSE
                     GGRLAGYLYVGSSFDVFAR"
BASE COUNT          499 a         1158 c         1115 g          586 t
ORIGIN      
        1 cgtcatgcgc cgcgtggtcg aggccggcgt gccgctgttt cagctcggca accgcgccta
       61 ctgcgaggag gagcgcgagg cacgcaggca atacaaggtg cacgccatcg acgccgacca
      121 gctggtaccg cagggcatcc acagcatcac cctgcctgcc gattttccgc cgcaggtttt
      181 ctttacgctg gacgtcgacg gcatcgatcc cgcggtgctg ccggccaccg gcacgccggt
      241 gcctggcggg ctgagctggt accagacgct cggactgttc gagtcggtgg cgcgccagcg
      301 ccgcatcgtc ggcttcgaca tcctcgagtt cgcaccgatc gcgggcttcc atgcctatga
      361 cttcgccgcc gcgctgctcg cctacaagct gatgggcatc gtgcagcgga cggccggagc
      421 cggcagcgag ccttgacaag ggcccgatcc ggcacccgtc ggggcgcgtg gttcgggttt
      481 ccggggccgc taccagctga agggcttccg caccggcacg atcgatgacg gcgccacgat
      541 gtgggccgtg gcccccgcat acgatttcgc gcggcgcagc tgcgttggca tcacctgcgg
      601 cacgccgacg agactaagcg tatccagaca cgtacagccg agccctgcgc gcggcagcgg
      661 tcgtgcgcgt ggcatcatgt tggcctttgg ctgacccagg acgtccatgc agcgcgacct
      721 cgtccgcacg atactcgaag tgctcgtcat tgcggcgctc gtcgtcgcgt cgctgtggat
      781 cctaagtccg ttcctcgcct cgatcgtctg ggcgacgatg atcgtcgtcg ccacctggcc
      841 gctgttcctc gcgctcgagg cgcgcctctg ggggcggcgc ggcctggcgg tgacggtgat
      901 gacgctcgcc atcctgctag tgctgttcgt ccccttgtcg ctcgccatcg gggcgctggt
      961 gcagaacgcc gacgcggcgg tggccttcgg caagcgcatt gcgcaggaag gcctgccgcc
     1021 gccgccgcgc agcgtggaaa cgttgccgct cgtcggcgag tatctcgcca aggcctggcg
     1081 cgcagtgctc gagctcggcc ttgccgacct gtggaaacgc attgcgccct atctgggtga
     1141 ggtcgccaag tggatagccg cgcgcgcggg cggcctcggc ctggttgcgg tccacctgct
     1201 gctcaccgcg gtgatttcgg cgatcctcta cgtaaacggc gaagcggcgg cgcgcatggc
     1261 acaccgcttc gccacgcgcc tcgccggcaa ggccggcgag gcttccgtcg tgctcgccgg
     1321 acaggcgatc cgcgccgttg ctatcggcat cgtggtcacg gccctcatcc aatccgtggt
     1381 cggcggcatc ggcctgctcg tcgccggcgt gccgcacgtc ggcatgctcg cggcgctcat
     1441 gttcatgttc tgcatcgcgc agctcggccc gggcttcgtc ctgttccccg ccgtagcgtg
     1501 gatgtactgg cagggcgtgc acggctgggc gaccttcctg ctggtgtggt cggtcggcgt
     1561 ggtgctgatg gacaacttcc tgcgcccgat cctcatccgg cgtggcgctg acctgccgtt
     1621 gctcttgatc ttcgccggcg tgatcggcgg gctgctggcg atgggtctgc ttggcctgtt
     1681 cgtcggaccg gtggtactcg cggtgaccta ccgcctcacc gaggcctggg tggacgaggc
     1741 cctgccgcag gcggcgcccg ccacaccgac ggctcagcgc gcaaacacat caaacgacga
     1801 gccgacgtaa agatagcctg cgagccgtcc gccctccgag tagccggcgc ctgcgtaaag
     1861 cgggccgagc gtgctgtcga tgatcaggta ggccgaccct gaccagatca cgccggacgc
     1921 gggtgtgcgg tcgaggcgct tgaacacgtt ccccgcttcc acaccgccac cgagatacac
     1981 cccggtgccc agcgttccgg tcagatcgaa gatgcgccgc ctgaagcccg cagtgccgag
     2041 caggtactcg cgaccacgca atgcgccgat ctgatagccg gagaaattgt tcagaccgcc
     2101 gagcgcaaat tgatcgtaga ccggagtcgc gctgccgaag tccgtgccgc cgcgcacggc
     2161 gaattcgacg acgttgcgcc cgtaggaaac gaccgagcgc cagtagagcc gacccttgcg
     2221 gtactcgtcg tcggcaccaa ggcttttgcg cgactcgtag tacctgatca gcccgcggcc
     2281 gccgcgcgtc gggaacaccg cgttgtcgag ctgatcgtat tccaagcgcg tttgccattg
     2341 gccgatcgcg atgcgctggc cgctgacggc aagcggattg ccgatgtcga cgtcgatgtc
     2401 ggcgcggccc cgatcaaggc cgaagcgcca ctcgccccag gtgtcgaacg cgatgccgac
     2461 gtcggccgca atgccgtatc gttccacctc gagtcgtgcg acccgctgct cgtcaaggaa
     2521 cagatcctcg aaggagctcg agaagtacac gcgtggcgca acgaagaagc gttgcgcctc
     2581 gtcgatcggc tggtagaact cggaggccag cagcgccggc tggccgacct gcagcgtgtt
     2641 gcgccactcg ccacccagcc ggttgaccca gctgcgacgg tgatcaacgc ggatgcccca
     2701 gcgcgtgtcg ccctggaaat cggtctgcat attgaggtcg aacagcagct ggttcgtgcc
     2761 gaggtttttc tcgcgcaccg tgtaggtgag cacgttgcga ccgtcttcgc gctgcatgtg
     2821 gaaatccacg ttctcgaagt caccccgacc gacgagtcgc gtggtgtcgg cggacgcctt
     2881 ttccgggtcg aacgcatcgc ccggcttcat ggcgatgaaa tggcgcagca cctccggatt
     2941 cacgtcttcg gtgccctcga tgcgcacctc gtcgatgcgt tcgactctgg ccaccgatac
     3001 gtccccgaga ccggcgcgcc aggcggcgta ctgcgcgcgc gaaaggctgt agcgccccag
     3061 ccgcgccgcc agcttgcgcg cagccgcctc tccgatcgga atcgcttcgg gaatgcggtc
     3121 gaagctcgtc gccccgatgc cgtcgagcac caccttcacc gcaacatcct gcttgccgag
     3181 cgtcgcgagc tgggcgcgtt cgttcagttc ggtgccgagc gaggcggcgc ggccgagcac
     3241 cgatagcgcg tccttcaagt cgccgcgcgt gggcggcgga ttgccgaccg tcacggcgag
     3301 cacgacatcg ccgcaggcct tgcgcgccac gtcgaccggc aggttgcgca cgaacatg
//
LOCUS       DATDAA010000366        13241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_131681,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000366 DATDAA010000000
VERSION     DATDAA010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13241
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131681"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2107
                     /locus_tag="VIS73_10900"
     CDS             <1..2107
                     /locus_tag="VIS73_10900"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY5763702.1"
                     /translation="RRRVHEDERRGAARTVAETEARIKALEALQQRVQGSGRLSEWLR
                     QHNLVGATPLWRHLRVDAGWEDALEAVLRERLTAISVGDAERVLEWATERSDAKLALI
                     LPDTKTGATAADAADALLTKVRCDDTQLMSVVREWLRGVRVANDLASALAGRTSLAEG
                     ESLVVPAGHLVGRHSVQLFAADEAQHGLLERQREIESLQAALVDQQAALEAVDAALEA
                     IDGERAALDEQISAQREAMEAARNRTHQIQVSLLRIEQIVERYRERHAQIEDGLAQFV
                     AEEETARGALMEAESAMTEARQEIEALQTGYNEAREALEKAERQLRDERESLAAAERE
                     WRESEFAIRECNGKIDEAENVQEQAKSQLARIDSDEAGARVEIAHNDDEDLEPKRQAA
                     LEHQVECERLLAEKREQVAGAENELRSLDEHRLQVEQGLEPLRERVGELKLKAQAATL
                     NAAQYQEQLSAANADEEALAPMLTGARAGSLQSAITGLQRDIEALGPVNLAALDELNT
                     ARERKGYLDDQANDLNEAVETLENAIRRIDRETRELLKATFDSVNTHFGQLFPALFGG
                     GRAELVMTGEEILDAGVQVVAQPPGKKNASIHLLSGGEKALTAIALVFGLFQLNPAPF
                     CLLDEVDAPLDDTNTERFCKMVSQMSKQTQFLYISHNKITMEMAEQLVGVTMPESGVS
                     RIVEVDIDEALRLRDQEAA"
     gene            2113..3195
                     /locus_tag="VIS73_10905"
     CDS             2113..3195
                     /locus_tag="VIS73_10905"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HEY5763703.1"
                     /translation="MSELQLAIALLGALGIAAVVAYNAWDSRRHRHLGDRVLPRMAGD
                     PLFDGDQKPARAGESEATRDAEPAARVEPVIDTAPGRAAAADRPEPHLLSPDFDFVLS
                     LTTLDPVSGERLMSLAGELSRANQRRVAVLGLDEVSRRWQAADGTGAYLAMLAGLQLT
                     DRRGAADEAEIALFSGAVRRFADEVMAVVDAPPLQQAAEQARQLDGLCARVDTQVGIN
                     VVGRNMAFSAARLRTLIERNTADFAEDGSALVTDAEGNPQFVIFDHDAQSLAPDSIAS
                     ATIRGLTFLLDVPRVADGARVFARMVQIARDFAGTLGGELVDDQRLPLTDAALDPIGR
                     QIAQQQELMAASGITPGSALARRLFA"
     gene            3195..5237
                     /gene="ligA"
                     /locus_tag="VIS73_10910"
     CDS             3195..5237
                     /gene="ligA"
                     /locus_tag="VIS73_10910"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY5763704.1"
                     /translation="MPVPSAVAAEIARLREEIDAHNQRYYVLDAPTISDAAYDALFRR
                     LQELEAEYPEALTPDSPTQRVGGAATATFSPVRHAVPMLSIRTETDTEAAAAFQFDAR
                     IRRELALDESAAAIEYLAELKFDGLAVNLRYEKGRLVRAATRGDGEIGEDVTANVRTI
                     AAIPSRWQGSPIPEVVEVRGEVFMARAAFEQLNLQQQERDEKVFVNPRNAAAGSLRQL
                     DPKISARRPLDFFAYGIGEVVGAELPPTQHALLDWLAAAGLPVCDERRVCAGPQALAD
                     FHARIGELRAQLPFDIDGVVYKVDALALQAKLGFVTREPRWAVAHKYPPQEETTTVLG
                     IEVQVGRTGKLTPVARLAPVFVGGVTVTNATLHNEDEIRRKDVRIGDTVVVRRAGDVI
                     PEVVGVIADKRPADSREFVMPAACPICASPAVRVEGEAATRCSGGLHCPAQRKQALLH
                     FAQRKAINIDGLGEKLVDQLVDSGRVTTPADLYALTAGDLAGMERMGEKSTANLLSAI
                     VASRDTSFARFIFSLGIPNVGEATAKDLARAFGRLDGLVQADEAALELVPDVGPIVAR
                     SIRQFFADNDNRAVIAALVDPKRGGIHWPDAEATVASGICAGKTFALTGTLPTLSREA
                     ATALIEAAGGRVSGSVSKKTHYLVAGADAGSKLEKARTLGVAILDESQLKAMVGED"
     gene            5237..6109
                     /gene="galU"
                     /locus_tag="VIS73_10915"
     CDS             5237..6109
                     /gene="galU"
                     /locus_tag="VIS73_10915"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY5763705.1"
                     /translation="MSAKRTVRKAVFPVAGLGTRFLPATKASPKEMLPVVDKPLIQYA
                     VEEAIAAGITDMIFVTGRTKRSIEDHFDKAYELETELELKGKHALLDQVRSILPANVN
                     CIYIRQTEPLGLGHAVLCARPIVGDEPFAVLLADDLLDATVPVMKQMADVYGRFGCSA
                     VAVQDVPRADTASYGIVDASPMAERIERVAAIVEKPKPADAPSTLAVVGRYLLTPRIF
                     DHLEKLGRGSGGEIQLTDGISNLLKEEEVLAYRYEGVRYDCGSKLGYLKATVALGERH
                     PEVGEAFREFLESR"
     gene            6109..6678
                     /locus_tag="VIS73_10920"
     CDS             6109..6678
                     /locus_tag="VIS73_10920"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEY5763706.1"
                     /translation="MAEKPSNKVGAEEARQILAGADLIHDAETVGRAIDQLAEDLTAA
                     VGQDNPLVLCVMTGGVVLTGQLLPKLAFPLEFDYLHATRYRQGTSGSTLSWRAAPWTN
                     VRDRCVLVLDDILDQGITLAAVKNRILQMGAARCVCAVLADKECSEARPVAADFAALK
                     VPDRYVFGMGMDIYGAWRNLPAIYALKDG"
     gene            6671..7378
                     /locus_tag="VIS73_10925"
     CDS             6671..7378
                     /locus_tag="VIS73_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763707.1"
                     /translation="MAEAAGAQPAIDRWAHEAAIHDAAGDYAWLAERLPGTRVIEIGC
                     GSGHASVAMLAREVELLVIEPLAACRQMLAERLAAAAIDGGRVRILDASADSLDSSAR
                     EALAAFAPDCIVCWLAGGSAEQLAQAGASAATDPVKAFREHLHRQLAELAAALPSVRA
                     VHLADRTAFPWQIKDTARDTLVSYHRASGFKDLPFTISRDDALFRRLDVNRWSPELRR
                     RGGPSPVLGSLIARRSA"
     gene            7394..8155
                     /locus_tag="VIS73_10930"
     CDS             7394..8155
                     /locus_tag="VIS73_10930"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HEY5763708.1"
                     /translation="MLAIIGGSGLTRLSSLDVSHREIARTPYGEASCPLVIGSLEGYP
                     AVFLARHGFGHTIPPHEVNYRANLWALRDRGAKAVVSIASVGGIREDLRPGDLLIPDQ
                     IIDYTWGRKSTFHEGDGCTVHHVDFTLPYDSALRDRLIRVASGAGIGFAAGGVYAATQ
                     GPRLETAAEINRLARDGADVVGMTGMPEAALAREIGLPYAAINVVANYAAGRGDSSQA
                     IRLDAIQPILDESMGRVRQLLQVFARDLASDLKQV"
     gene            complement(8135..10732)
                     /locus_tag="VIS73_10935"
     CDS             complement(8135..10732)
                     /locus_tag="VIS73_10935"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEY5763709.1"
                     /translation="MPADPQTIARLRDSGREGLAALAEGFARDGNPRKFVEARAEQVD
                     RILVELWTASGLADRATMVAVGGYGRGELYPASDVDILILLPEQAPRETLGAIESLIG
                     TFWDIGLEVGHSVRSIADCLAVATGDITIETTLIEARHLAGDRALFDALVKAVRELVQ
                     PGAFFKAKRLEQNERYLRFQETPYSLEPNCKESPGGLRDLHVILWIVKAAGLGNRWVD
                     LEKQGLITAEEVRSLRQCERFLQDLRIRLHLQVGRREDRLVFDVQTKLAESLGYRASE
                     TRRSSEVMMQAYYRNAKLVTQLNTILLQNLGTLILGKSGAEITPIDDRFQRVGNLLDL
                     LDEKLFQRQPQAILASFATIQRIPELKGMTARTLRALWRARNRIDDAFRADPRNRATF
                     LAILQSPHGIVHEFRRMNQYGILGRYLPAFGKIVGQMQHDLFHVYTVDQHILMVMRNV
                     RRFTMDEFAHEHPLESRLISAFPRHWLLYVAALFHDIAKGRGGDHSQLGMVDADDFCR
                     VHCLSAEDTELVVWLVREHLTMSHIAQKSDITDPRVVDDFARFVGDERHLTALYLLTV
                     ADIRGTSPKVWNAWRAQLLATLFRATRAVLRYDKEAPPAQGIVEERQQEAMELLRRFA
                     LAESVQEHLWNELDTAYFLRHDADEIAWHTRALHYRTLGREPVIKARLNRHANDDDEI
                     TFDAGLQVMVYTPDEPTLFLRLVGFFARTGFSIVDARIHTTRHGYALDTFVLLDVLDR
                     DNDRELLNYVEHELSAHLKGSAPPLIPSSGRVSRQVRHFPITPEVTIRPDDRGDHFVL
                     TVTAADRPGLLFGIAEILAAHRVALHSAKIATLGDRISDTFLVSGPALTQSGDRIRLE
                     TALLDLLQV"
     gene            complement(10750..11550)
                     /gene="map"
                     /locus_tag="VIS73_10940"
     CDS             complement(10750..11550)
                     /gene="map"
                     /locus_tag="VIS73_10940"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY5763710.1"
                     /translation="MTVVIKTPDEIEGMRVAGRLASEVLDFITPHVRAGVTTGELDRL
                     CHDYMVDTQKTIPAPLNYAPAGYTPYPKSICTSVNHQVCHGIPGDRKLRNGDIVNLDI
                     TVIKDGLHGDTSRMFMVGEPSTLARRLCQVTFECMWRGIARIRPGAHLGDIGEAIQRH
                     AEINGYSVVREFCGHGIGRRFHEEPQVLHYGRAGSGLRLEAGMIFTVEPMINAGKAAI
                     RSLGDGWTVVTKDHSLSAQWEHTVLVTPTGYEVLTVSAESPPAPETPA"
     gene            11852..12613
                     /gene="rpsB"
                     /locus_tag="VIS73_10945"
     CDS             11852..12613
                     /gene="rpsB"
                     /locus_tag="VIS73_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY5763711.1"
                     /translation="MSVTMRQMLEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLEQ
                     TLAKYNEAMAFVRKLSANRGNVLFVSTKRQAREIIAEEAARAGAPFVDQRWLGGMLTN
                     FKTVKLSIKRLKDMEQISTEGGLERMSKKEALTMSRDLEKLQKSLGGIKEMNALPDAI
                     FIVDVGYHKIAVTEAAKLGIPVIAVVDTNHSPEGISYVIPGNDDSSRAIRLYARGVAD
                     AILEGRAQSTQEIVSAGKDEDEFVEVAEGEETGEA"
     gene            12721..>13241
                     /gene="tsf"
                     /locus_tag="VIS73_10950"
     CDS             12721..>13241
                     /gene="tsf"
                     /locus_tag="VIS73_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY5763712.1"
                     /translation="MAEITAAMVKELREKTDAPMMECKKALTEAAGDIAKAEEILRVK
                     LGNKASKAAARVAAEGIVGISITADGKVGAIIEVNSETDFVAKNDDFIALTNNCAQLV
                     VDHNPTDVAALSGLPMGEGTVESTRTALVGKIGENMSIRRFNRVAAKGSLASYIHGGA
                     KIGVLVDLVGGDE"
BASE COUNT         2329 a         4257 c         4464 g         2191 t
ORIGIN      
        1 ccgccgccgc gtccacgagg acgagcgacg cggcgcggcg cgcaccgtcg ccgagaccga
       61 agcgcgcatc aaggcgctcg aggcgctgca gcagcgcgtg cagggcagcg gccgtctcag
      121 cgagtggttg cgccagcaca atttggtcgg cgcgactccc ctgtggcggc acctgcgcgt
      181 cgatgccggt tgggaggacg cgcttgaagc ggtgctgcgc gagcgcctga ccgcgatctc
      241 ggtcggcgac gccgagcgcg tgctcgaatg ggcgaccgag cgttccgacg ccaagctggc
      301 gctgattctt cccgatacga agacaggcgc caccgcggcc gatgccgccg atgcgctgct
      361 gaccaaggtg cgctgcgacg acacgcagct gatgagcgtg gtccgcgagt ggctgcgcgg
      421 cgtacgcgtc gccaacgatc tggcgagcgc gcttgccggc cggacttcac tggccgaagg
      481 cgagtcgctg gtggttccgg cgggacatct ggtcggccgt cacagcgtcc agctgtttgc
      541 tgccgacgag gcgcagcacg gtttgctcga gcgccagcgc gagatcgaat cgctgcaggc
      601 ggcgctggtc gaccaacagg cggcgctcga ggccgtcgat gcggcgctcg aagcgatcga
      661 cggcgaacgc gccgcgctcg acgagcagat ctcggcgcag cgcgaggcga tggaagccgc
      721 gcgcaatcgc acccaccaga ttcaggtttc gctgctgcgc atcgagcaga tcgtcgagcg
      781 ctaccgcgag cgacacgccc agatcgaaga cgggctggcg caatttgtcg ccgaggaaga
      841 gacggcgcgt ggcgccctga tggaagccga gtcggcgatg accgaagcgc gccaggagat
      901 cgaggcgctg cagaccggct acaacgaggc gcgcgaggcg ctggaaaagg ccgagcgcca
      961 attgcgcgac gagcgcgaat cgctggccgc cgccgaacgc gaatggcgtg agtcggaatt
     1021 cgccatccgc gaatgcaacg gcaagatcga cgaggccgaa aacgtccagg agcaggccaa
     1081 gtcgcaactg gcccgcatcg acagcgacga ggccggcgca cgcgtcgaga tcgcccacaa
     1141 cgacgacgag gatctcgagc cgaagcggca ggcagcgctc gagcatcagg tcgaatgcga
     1201 acggctgctg gccgagaaac gcgagcaggt cgctggcgcc gagaatgaac tgcgctcgct
     1261 ggacgagcac cggctgcagg tggagcaggg tctggagccg cttcgcgagc gcgtcggcga
     1321 actcaagctc aaggcacagg cggcaacgct caacgccgcc cagtatcagg aacagttgag
     1381 cgccgccaat gccgacgaag aggcgctggc accgatgctc accggcgctc gtgccggctc
     1441 cttgcagtcg gcgattaccg gccttcagcg cgacatcgaa gcgctcggcc cggtcaacct
     1501 cgcggcactc gacgaactga acacggcgcg cgagcgcaag ggctacctcg acgatcaggc
     1561 caacgacctt aacgaggcgg tcgagacgct ggagaacgcc atccgccgca tcgaccgcga
     1621 gacacgcgag ctgctcaagg ccaccttcga cagcgtcaac acgcatttcg ggcagctctt
     1681 cccggcgctc ttcggcggcg gtcgcgccga gctagtgatg accggcgagg aaatcctcga
     1741 tgccggcgtg caggtcgtcg cccagccccc gggcaagaaa aatgcgtcga tccacctgct
     1801 ctcgggcggc gagaaggcgc tgaccgcgat tgcgctggtg ttcggcctgt tccagctcaa
     1861 cccggcgccg ttctgcctgc ttgacgaggt cgatgcaccg ctggacgata cgaacactga
     1921 gcgcttctgc aagatggtta gccagatgtc gaagcagacg cagttcctct acatcagcca
     1981 caacaagatc acgatggaaa tggccgaaca gttggtcggc gtgaccatgc ccgagtcggg
     2041 cgtgtcgcgg atcgtcgaag tcgatatcga cgaagccctg cgcctgcgcg atcaggaagc
     2101 ggcctgagcc cgatgagcga actccagctc gccatcgctc tgctcggcgc tctcggtatc
     2161 gccgcggtcg tcgcgtacaa cgcttgggat tcgcgtcgtc accgccatct cggcgaccgt
     2221 gtgctgccac gcatggccgg cgaccctttg ttcgacgggg atcagaagcc ggcgcgggcc
     2281 ggcgagtcgg aagcgacccg tgatgccgag ccagccgcgc gcgtcgagcc ggtcatcgat
     2341 accgcgccgg gtcgcgccgc cgccgccgat cgccccgagc cgcatctgct gtctccggat
     2401 ttcgatttcg tgctgtcgct gaccacgctc gacccggtct cgggcgagcg gctgatgagt
     2461 ctggccggcg aactcagccg cgcgaaccag cgccgcgtcg ccgtactcgg actcgacgag
     2521 gtcagccgac gctggcaagc cgccgacggc accggcgcct atctcgcgat gcttgctggg
     2581 ctgcagctta ccgaccgtcg tggtgccgct gacgaggccg agatcgcgct gttctcgggc
     2641 gccgtgcgcc gcttcgccga tgaggtgatg gccgttgtcg atgcgccacc gctgcagcag
     2701 gccgccgagc aggcgcgcca gctcgatgga ctatgcgccc gcgtcgatac gcaggtcggc
     2761 atcaacgtcg ttggccgcaa catggccttc tcggcagcgc gcctgcgcac gctgatcgag
     2821 cgcaataccg ctgatttcgc cgaggacggc agcgcgctcg tcaccgatgc cgagggcaac
     2881 ccgcagttcg tcatcttcga tcacgacgcc cagtcgttgg cgccggacag tattgcctcg
     2941 gcgacgatcc gcggcctgac cttcctgctc gacgtgccgc gcgtcgccga cggtgcccgt
     3001 gtctttgcgc gcatggtcca aatagcgcgc gactttgccg gcacgctggg cggcgaactg
     3061 gtcgacgacc agcgcctgcc gctcaccgac gccgcgctcg atccgatcgg tcgccagatc
     3121 gcccagcagc aggagctcat ggcggcaagc ggcatcacgc cgggtagcgc gctggcacgc
     3181 cgcctgttcg cctgatgccg gttccgtccg cggtcgccgc cgagattgcc cggctgcgag
     3241 aggaaatcga cgcccacaac cagcgctact acgtcctcga tgcgccgacg atttccgacg
     3301 ctgcgtacga cgcgctgttc cgccgcctgc aggagcttga agccgaatac ccggaagcct
     3361 taacgccgga ttcgccgacg cagcgtgtcg gcggggcagc cacggcgacc ttctcgccgg
     3421 tccgccatgc ggttccaatg ctgtcgatcc gcaccgagac cgataccgag gctgccgcag
     3481 ccttccagtt cgacgcgcgc atccgtcgcg agctggcgct cgacgaatcg gccgccgcca
     3541 tcgagtatct cgccgagctg aagttcgacg gcctggccgt caacctgcgc tacgagaagg
     3601 gccgcctggt gcgcgcggcg acacgcggcg acggcgagat cggcgaggac gtcaccgcca
     3661 atgtgcggac gattgccgcc attccgtcgc gctggcaggg gagtccgatt cccgaggtgg
     3721 tggaggtgcg tggcgaggtg ttcatggccc gcgctgcctt cgaacagctc aatctccagc
     3781 agcaggagcg cgacgaaaaa gtcttcgtca atccgcgcaa tgcagcggcc ggcagcctgc
     3841 gccagcttga tccgaagatc agcgcgcggc ggccgctcga tttctttgcc tacggcatcg
     3901 gcgaggtcgt cggcgccgag ctgccgccga cacagcacgc gctgctcgac tggctggccg
     3961 ctgccgggtt gcccgtgtgc gatgagcggc gcgtctgcgc gggaccacag gcgctcgccg
     4021 attttcacgc gcggatcggc gagttgcgcg cgcaactgcc cttcgacatc gatggggtcg
     4081 tctacaaggt cgacgcgcta gcgctgcagg cgaaactcgg gttcgtcact cgcgagccac
     4141 gctgggccgt ggcgcataag tacccgccgc aggaggaaac gacgacggtg ctgggcatcg
     4201 aggtgcaggt cggccgtacc ggcaagctga ccccggtggc gcggctcgcg ccggtattcg
     4261 tcggcggtgt cacggtcacc aacgccacgc tgcacaacga ggacgagatc cgccgcaagg
     4321 acgtccggat cggcgacacg gtcgtcgttc gccgcgccgg cgacgtgatt cccgaggtcg
     4381 tcggcgtcat cgccgataag cgcccggccg acagccgcga gttcgtcatg ccggcggcct
     4441 gcccgatttg tgcctcgccg gcggtgcgcg ttgaagggga ggcggcaacg cgctgtagcg
     4501 gcgggctgca ctgcccggcg cagcgcaagc aggcgctgct gcactttgcg cagcgcaagg
     4561 cgatcaacat cgacggactc ggcgagaaac tcgtcgatca gctggtcgac tccggccgcg
     4621 tcaccacgcc ggccgacctc tatgcactaa cggccggcga tcttgccggc atggagcgca
     4681 tgggcgagaa gtcgacggcc aacctactgt cggcgatcgt cgcgagtcgc gatacgagct
     4741 tcgcgcgttt cattttttcg cttggaatcc ccaacgtcgg cgaggcgacg gccaaggatc
     4801 ttgcgcgcgc ctttggccgg ctcgacggcc tggtgcaggc cgacgaggcg gcgctcgaac
     4861 tggttcccga tgtcgggccc atcgtcgccc gcagcatccg ccagttcttt gcggataacg
     4921 acaaccgggc ggtgatcgcg gcgctggttg acccaaaacg tggcggaatt cactggcccg
     4981 atgccgaagc caccgtcgct tcgggcattt gcgctggcaa gacatttgca ttgaccggta
     5041 cactgccaac cctgagccga gaggcggcga cggcactgat cgaggccgcc ggtggccgag
     5101 tcagcggctc ggtttcgaag aagacgcatt acctggtagc cggcgctgac gccggcagca
     5161 agctcgagaa ggcgaggacg ctcggcgtgg cgatcctcga tgaatcacaa ctcaaagcga
     5221 tggtgggaga agattgatgt cggcgaaaag aacagtccgc aaagcagtat ttccggttgc
     5281 cggtcttggc acgcgctttc tgccggcgac caaggccagt ccgaaggaga tgctgccggt
     5341 cgtcgacaag ccgctgatcc agtacgcagt cgaggaggcg atcgctgccg gcattaccga
     5401 catgatcttc gtcaccggcc gcaccaagcg ctcgatcgag gaccatttcg acaaggccta
     5461 cgaactcgag accgaacttg aactcaaggg caaacacgcg ctgctggacc aggttcgcag
     5521 catcctgccg gccaacgtca attgcatcta catccgccag accgagccgc tgggcctcgg
     5581 ccatgcagtg ctctgcgcca ggcccatcgt cggcgacgag ccctttgccg tgctgctcgc
     5641 cgacgacctg ctcgacgcaa cggtgccggt gatgaaacag atggccgatg tctacggccg
     5701 ctttggttgc agcgcggtcg ccgtgcagga tgtaccgcgc gccgataccg ccagctacgg
     5761 catcgtcgat gcctcgccga tggccgagcg tatcgaacgg gtagcggcga tcgtcgagaa
     5821 acccaagccc gccgatgcac cgtcgacgct cgccgtcgtc ggacgctacc tgctgacgcc
     5881 gcgcatcttc gatcacctcg aaaagctcgg ccgcggctcc ggcggcgaga tccagctgac
     5941 cgacggcatc tcgaacctgc tgaaggagga agaggtgctc gcctatcgct acgagggggt
     6001 ccgctacgac tgcggttcca agctcggcta tctgaaggcg accgtggcgc tcggcgagcg
     6061 gcacccggag gttggcgagg cctttcgcga attcctggag agccgttgat ggccgaaaag
     6121 ccaagcaaca aggtgggcgc cgaggaggcg cggcagatcc tcgccggcgc cgacctgatc
     6181 cacgacgccg aaacagtcgg ccgggccatc gatcaactcg ccgaagacct gacggcggcg
     6241 gtcggccagg acaatccgct ggtgctctgc gtgatgaccg gcggcgtcgt gctcaccggt
     6301 cagttgctgc ccaagctcgc ctttccgctc gaattcgatt acctgcacgc tacccgctac
     6361 cgtcagggga cgagtggcag cacgctgtcc tggcgcgcgg ccccgtggac caacgttcgg
     6421 gatcgctgcg tgctggtgct cgacgacatc ctcgaccagg gcatcacgct ggccgctgtg
     6481 aagaaccgca tcctgcagat gggcgcggcc cgctgcgtgt gcgcggtgct cgccgacaag
     6541 gagtgcagcg aagcgcgccc ggttgccgcc gacttcgccg cgctcaaggt gcccgaccgc
     6601 tatgttttcg gaatgggcat ggatatctac ggcgcctggc gcaacctgcc agcgatctac
     6661 gcgctgaaag atggctgagg cggccggcgc acagccggct atcgatcgct gggcccacga
     6721 ggcggcgatc cacgatgcgg cgggggacta cgcctggctg gccgaacggc tgccgggcac
     6781 tcgtgtcatc gagatcggtt gcggttcggg tcacgccagc gtcgcgatgc tggcgcgcga
     6841 agtcgaactg ctggtgatcg agccactggc ggcgtgcagg cagatgctgg ccgagcggct
     6901 cgcggcggca gccatcgacg gcgggcgggt gcgtatcctc gacgcatcgg ctgactcgct
     6961 cgactcgtcg gcacgcgagg cgctcgccgc gttcgcccct gactgcatcg tctgctggct
     7021 cgccggcggc agcgccgaac agcttgccca ggctggtgcc agtgcggcga ccgatccggt
     7081 caaggccttc cgcgagcacc tgcaccggca gctggccgag ctggcggcgg cgttgcccag
     7141 cgtgcgcgcc gtgcatctgg ccgaccgcac ggcatttccg tggcagatca aggacaccgc
     7201 gcgcgacacg ctggtcagct accatcgcgc ttcgggtttc aaggacctgc cattcacgat
     7261 ttcccgcgat gacgcgctgt ttcgccggct cgacgtgaat cgctggtcgc cggagctgcg
     7321 gcggcgcggc ggcccaagcc cggtgctcgg atccctgatc gcccgccgat ccgcctgact
     7381 aggagaacaa caaatgctcg ccatcattgg tggtagcggc ctgacgcgac tgtccagcct
     7441 cgacgtatcg caccgcgaga ttgcccggac cccctacggc gaggcctcct gcccgctcgt
     7501 catcggcagc cttgagggtt atccggcagt gtttctggca cgccacggct tcggccacac
     7561 gatcccgccg cacgaggtga attaccgggc caatctctgg gctttgcgcg acaggggcgc
     7621 gaaggcggtt gtctcgatcg cctcggtcgg cggcatccga gaagacctgc ggccgggcga
     7681 cctgctgatt cccgaccaga tcatcgacta tacctggggc cgcaagtcga ccttccacga
     7741 aggcgacggc tgcaccgttc atcacgttga tttcaccctg ccttacgaca gcgccctgcg
     7801 agatcggctg atccgcgtcg cctcgggcgc cggcatcggc ttcgccgccg gcggcgtcta
     7861 cgcggcgacg cagggcccac gtctggaaac ggcggccgag atcaaccgtc tggcgcgcga
     7921 cggagccgac gtggtgggga tgaccggcat gccggaagca gcgctggcgc gcgagatcgg
     7981 actgccctac gcggcgatca acgtcgtcgc caactatgcg gccggtcgcg gggacagcag
     8041 ccaggcgatc cggctcgatg caatccagcc gatcctcgat gagtctatgg ggcgtgttcg
     8101 acagctgctg caggtgtttg cccgcgacct cgcctcagac ctgaagcagg tctagcagcg
     8161 ccgtttcgag gcggatgcgg tcgccgctct gggtgagcgc cgggccgctg acgagaaagg
     8221 tgtcggaaat ccggtcgccc agcgtcgcga tcttcgccga gtgcagcgcg acgcgatggg
     8281 cggcaagtat ttcggcgatg ccgaagagca ggcccggtcg gtcggccgcg gttaccgtga
     8341 ggacgaagtg gtcgccgcga tcgtcagggc gtatggtgac ttcgggcgtg atcgggaaat
     8401 gcctgacctg ccgtgaaacg cgaccgctgg atggaatcaa cggcggtgcg ctgcccttga
     8461 ggtgcgcgga aagctcgtgc tcgacgtagt tgagcagttc gcgatcgttg tcgcggtcga
     8521 gtacgtcgag caggacgaag gtgtcgagcg cgtagccgtg ccgcgtcgta tggattcgcg
     8581 cgtcgacgat cgaaaagccg gtgcgcgcga agaagccaac cagccgcaga aacagcgttg
     8641 gctcgtcggg cgtgtagacc atgacctgca ggcccgcatc gaaggtgatt tcgtcatcgt
     8701 cgttggcgtg ccggttgagg cgggccttga taaccggctc gcggcccagc gtccggtagt
     8761 gcagggcgcg ggtgtgccag gcaatctcgt cggcgtcgtg gcgcaggaag tacgcggtat
     8821 caagttcatt ccataggtgt tcctggacgc tttcggctag cgcgaagcga cgcaacagtt
     8881 ccatcgcttc ctgttgcctc tcttcgacga taccctgcgc cggcggagct tccttgtcgt
     8941 agcggagaac agcccgtgtg gcgcggaaca gcgttgccag caactgggcg cgccaggcgt
     9001 tccagacctt cgggctggtc ccgcggatat cggcaacggt cagcaggtag agcgcggtga
     9061 ggtgtcgctc gtcacccacg aaccgggcga agtcgtcgac gacccgcgga tcggtgatgt
     9121 ccgatttctg ggcaatgtgc gacatcgtca ggtgctcgcg gacgagccag acgaccaatt
     9181 cggtatcttc ggccgacagg cagtgcacgc ggcagaagtc atcggcatcg accattccca
     9241 gctgcgaatg gtcgccgccg cgtcccttgg cgatgtcgtg gaagagggca gcgacgtaga
     9301 gcagccagtg gcgcgggaag gccgatatca ggcgtgattc gagcgggtgt tcgtgggcga
     9361 actcgtccat cgtaaagcgg cggacattgc gcatcaccat caggatgtgc tggtcgaccg
     9421 tgtaaacatg gaagaggtcg tgctgcatct ggccgacgat cttgccgaag gccggcaggt
     9481 agcggccgag aatcccatac tggttcatgc gacggaactc gtggacgatg ccgtgcggac
     9541 tctgcagtat ggcgagaaag gttgctctgt tacgcgggtc ggctcggaac gcgtcgtcga
     9601 ttcggttacg ggcgcgccac agcgcgcgca gggtgcgcgc cgtcatcccc ttgagttcag
     9661 gaatgcgctg tatcgtcgcg aagctcgcca gaatcgcttg cggctgacgc tgaaagagtt
     9721 tctcgtcgag cagatcgagc aggttgccga cgcgctggaa gcggtcgtcg attggcgtga
     9781 tttcggcgcc gctcttgccg aggatcagcg tgccgagatt ctgcagcagg atcgtgttga
     9841 gctgcgtcac cagcttggcg ttgcggtagt aggcctgcat catgacttcg cttgatcggc
     9901 gggtctcgct ggcgcgatag ccgagcgatt cggcgagttt ggtctgcacg tcgaagacca
     9961 agcggtcttc gcggcggccg acctgcaggt gcaggcgaat tcgcaggtcc tgcaggaaac
    10021 gttcgcactg gcgcaggctg cggacttcct cggcggtgat cagaccctgt ttttcgagat
    10081 cgacccagcg attgccgagt ccggcggcct tgacgatcca gagaatgaca tgaaggtctc
    10141 gcaggccacc cgggctttcc ttgcagttcg gctcgaggct gtacggcgtc tcctgaaagc
    10201 gcaggtagcg ttcgttctgc tccaggcgct tcgccttgaa gaaggcgccg ggctgaacca
    10261 gctcgcggac cgccttgacc agtgcatcga acagcgctcg gtcgccggcc aggtggcggg
    10321 cctcgatcag cgttgtttcg atggtgatgt cgccggtagc gacggccagg cagtcggcga
    10381 tgctgcggac gctgtggccg acttcgagcc cgatgtccca gaaggtgccg atcagcgact
    10441 cgatcgcccc cagcgtctcg cgcggggcct gctcgggaag caggatcagg atgtcgacgt
    10501 cggaagccgg gtagagctcg ccacgaccgt aaccgccgac ggcgaccatg gtcgcccgat
    10561 cagccaagcc gctggcggtc catagctcga cgagaatgcg gtcgacctgc tccgcgcggg
    10621 cctcgacgaa tttccgcgga ttgccgtccc gggcaaagcc ctcggcaagc gccgcaaggc
    10681 cttcccgacc gctatcgcgc aggcgggcga tggtctgggg atcggcgggc atcgaaggca
    10741 gtagcctggt caggccggcg tttccggggc cggcggcgac tcggcggaaa cggtcaggac
    10801 ctcgtagccg gtcggcgtca ccaacaccgt gtgttcccac tgcgccgaca ggctgtggtc
    10861 cttggtgacc acggtccaac cgtcgccgag cgaacggatc gccgccttgc cggcgttgat
    10921 catcggctcg accgtgaaga tcatcccggc ctcgaggcgc agtcccgaac cggcgcggcc
    10981 gtagtggaga acctgcggct cctcgtggaa tcgccggccg atgccatgcc cgcagaactc
    11041 gcggaccacc gaatagccgt tgatctcggc gtgccgctgg atcgcctcgc cgatgtcgcc
    11101 gaggtgggct cccgggcgga tgcgggcgat gccgcgccac atgcattcga aggtgacctg
    11161 gcacagccgg cgcgccagtg tcgacggttc gccgaccatg aacatacgac tggtgtcgcc
    11221 atgcaggccg tccttgatga cggtgatgtc gagattgacg atgtcgccgt tgcgcagctt
    11281 gcggtcaccg ggtatgccgt ggcagacctg atggttaacc gaggtgcaga tcgacttcgg
    11341 atacggcgta tagcccgccg gggcgtagtt gagcggtgcc gggatcgtct tctgcgtgtc
    11401 gaccatgtag tcgtggcaga ggcggtcgag ttcgccggta gtgacgccgg cgcggacgtg
    11461 gggggtgatg aaatcgagca cttccgaggc cagtctgccg gccactcgca tgccttcgat
    11521 ttcgtctggc gtcttgatca ctacggtcat ggcggatggt cggcaggtgc cgcgcaaaag
    11581 gttcgcagaa tagcgactcg ggtaagcatc ggcaaatcga cagcgcggcg ccgcagaggt
    11641 tctccgggca tttcacaaag tgcttcacct ctgattccgt gctagaatcc ggcggttttt
    11701 ttggtgcctg accggtattt tcggcggcat caaggaaatg cgaacgcggg gcgcaatccc
    11761 tgcgaattca cacactcggt cgaaagggtt ttccggaata tccggaaacg ttgaccgggt
    11821 ggcggaaaaa ccctttcagg agctacctgt catgtcagta accatgcgtc aaatgctgga
    11881 ggccggcgtt catttcggcc atcagacccg tttctggaac ccgaagatgg cacagtacat
    11941 cttcggcgcc cgcaacaaga tccacatcgt caacctcgag cagacgctcg ccaagtacaa
    12001 cgaggcgatg gccttcgtgc gcaagctgtc tgccaaccgc ggcaatgtgc tgttcgtcag
    12061 caccaagcgt caggcccgcg agatcatcgc cgaggaagcg gcccgcgccg gcgcgccctt
    12121 cgtcgaccag cgctggctag gtggcatgct gaccaacttc aagacggtca agctgtcgat
    12181 caagcgcctc aaggacatgg agcagatctc caccgagggc ggccttgagc ggatgtcgaa
    12241 gaaagaggcg ctgacgatgt cgcgcgatct ggaaaagctg cagaagagcc tcggcggcat
    12301 caaggaaatg aatgcgctgc ccgacgccat cttcattgtc gacgttggct atcacaagat
    12361 cgccgtcacc gaagccgcca agctcggcat tccggtgatc gctgtggtcg ataccaacca
    12421 ctcgccggaa gggatctcct acgtgatccc gggcaacgat gactccagcc gggcgattcg
    12481 cctctacgca cgtggcgttg ctgatgcgat cctcgagggc cgcgcccagt cgacgcagga
    12541 aatcgtcagc gccggcaagg atgaggacga gttcgtcgag gttgccgagg gcgaggaaac
    12601 aggcgaagcc tgattcacac ccacagaccg ggatcgcccg attacccagt cgggcatccc
    12661 gttttcttta tcggccgggg caatcgccgc ggccgcgaat tcgagatacg aggtacagga
    12721 atggctgaaa tcaccgccgc aatggtgaag gaactacgcg aaaagaccga cgctccgatg
    12781 atggagtgca agaaggcact gaccgaggca gcaggggata tcgccaaggc cgaggagatc
    12841 ctccgcgtca agctcggcaa caaggcgagc aaggccgctg cccgggtcgc cgccgaaggc
    12901 atcgtcggca tcagcatcac cgccgatggc aaggtcggtg ccatcatcga ggtcaattcg
    12961 gaaaccgact tcgttgccaa gaacgatgac ttcatcgcgc tgaccaacaa ctgcgctcag
    13021 ctcgtcgtcg atcacaatcc gaccgacgta gcagcccttt ccggactgcc gatgggcgag
    13081 ggcacggtcg agtcgacgcg cacggcgctg gtcggcaaga tcggcgagaa catgtcgatc
    13141 cgccgcttca accgtgttgc ggccaagggt tcgctcgctt cctacatcca cggcggcgcc
    13201 aaaattggcg ttctcgtcga tctggtcggc ggtgacgagc a
//
LOCUS       DATDAA010000367        12416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_60978,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000367 DATDAA010000000
VERSION     DATDAA010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12416
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60978"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VIS73_10955"
     CDS             complement(<1..645)
                     /locus_tag="VIS73_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEY5763713.1"
                     /translation="MQMDVVDYEIFGTDMQYVEVELDPGEAAVGEAGAMMYMQDGIEM
                     DTVFGDGSQQGGFFGKLMGAGKRLLTGESLFTTVFHNEGQGKKRVAFSAPYPGRIIPV
                     HLAEIGGTLICQKDSFLCAAKGVSIGIALQKRLGVGLFGGEGFIMQKLEGDGLAFVHA
                     GGTLLERTLQPGETLRVDTGCVVAFQTSVNFDIQYVGKIKSALFGGEGLFFATLR"
     gene            819..3242
                     /locus_tag="VIS73_10960"
     CDS             819..3242
                     /locus_tag="VIS73_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5763714.1"
                     /translation="MNPETDSMLADRLSASQLEAILEIADDAIVCVDRNLRIALFNRG
                     AERIFGYKANEAIGQPLNLLIPDGARPRHTAHVTDFHNSGASARQMAERSEISGRRKD
                     GTTFPAEATISQLAVDGGVIFTAILRDVTLRKAHEQELENARAVAEAATQAKSMFLAN
                     MSHELRTPLNAVVGMTSLLLDTAITEEQRDYAQTIRASSETLLAIINDILDYSKIEIG
                     RLEIESQPFDLRRAIEESLDLVAPIAGEKNLNLAYLIAEGVPAGIQGDVTRLRQILVN
                     LLANATKFTHHGEVLVSVEGSPLDNGHFELHFSVADTGIGIPVDRIDQLFQSFTQVDA
                     STTRKYGGSGLGLAISRRLAEMMGGRMWVDSVAGEGSTFHFTIEAAPIDARSAHNVLQ
                     ERSAALSGKRLLIVDDNTTNRRILVKQALLWGMLPSAAATPVEAIDLIRHGHAFDAGI
                     LDMHMPDMDGLMLAREIRRLRDANSLPLIMLTSLVQRPKPAEAEQLAFSAFLNKPIKP
                     TALFDALAVALRLPPASAPAVSTTTRRVRGNGSRNLRLLVAEDNTVNQKVMQQLLAHL
                     GYQADVASTGGEALDALERQPYDVVLMDVQMPEMDGLEATRRIRTHFGASPYIVAMTA
                     NAMVGDREECLKAGMNDYLPKPVELTQLSIVLDRAAAAAGEAPNAVTTDAAETLAADG
                     TAHETAPRIIDRRQIDQLRSLEGPDNPNLVAEIVDMFLRDAPQHLDGLAAAAAAGDAA
                     ALKAIAHRFLSSIENLGAIAMRERCMTLEALGREGRSDGAAELVARLRDDFAAAREAL
                     LSLLGATAR"
     gene            3239..4402
                     /locus_tag="VIS73_10965"
     CDS             3239..4402
                     /locus_tag="VIS73_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HEY5763715.1"
                     /translation="MKPDFDVAILGGGLAGASLALALRASGRRIAVVDARPANASVAA
                     SDPRAYAISPTAMEFLETIGVWRHLDRQCVGPVVAMDIRGDRGGRLRFSAFEARREAL
                     AWMVEAETLEAEIRETARRQAGIDWLAPATPTAMTQNTDAATLTLSTGVRLSARLLVG
                     ADGRDSWLRETAGIAATTTGYGEQGIAACYACEQAHGDTARQWFHGDSVLAWLPLPEQ
                     RISIVWSAPDSLATHLTALDPEALALRVGAAGDHVLGRLTPLGPARGFPLRLIRVPDP
                     IASRIALIGDAAHGIHPLSGHGINLGFSDARELAAAITDCPPGGDVGGERVLRRYRRR
                     RAEEVRLLQEATHGLRQLFASRAPGLSLLRNLGLSVTDRLPRVKSLLTRYAMG"
     gene            4433..5149
                     /locus_tag="VIS73_10970"
     CDS             4433..5149
                     /locus_tag="VIS73_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEY5763716.1"
                     /translation="MSFPRNNPFAAALVALALALPLAAQANDKVSAAFEKAFRVKPEK
                     VTRIEAIGLYEIVVGGDILYTDEKVTHLIQGSLIDVKTRTNLTEERLNKLLAINFAEL
                     PLDLAVKQVRGNGKATLVTFEDPNCGYCKRLARDLTNLEDTTIYTFLYPILSADSTEK
                     SRAIWCAKDRARAWNDWMIDGKAPTAATCDAPLEQLVELGQRYRVTGTPTIFFTDGSR
                     APGAIPLQQIRERLAKIAGK"
     gene            5239..6108
                     /locus_tag="VIS73_10975"
     CDS             5239..6108
                     /locus_tag="VIS73_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763717.1"
                     /translation="MGLLNGLLGRRKASPTAAHPLLEHVPAVLGDEILAAPDFAETLI
                     PALEHADAYLKNQVAAIPGPLTLSSATRPTDPLMSRIFADDAELRAGLGRSLEMKESI
                     VQLAVAGHVHSYALLGMRLKRGVTPAPGEAPVFADHTVKAIADTEAHARGELRVSALR
                     RIIKNYDQHLAKLREKQQLLKLEWNIENNPAGKDDGNEFVHAKTELTPGNMVRGLAAW
                     LRRPEEVLKLTNGDVRIPGRSADGAQSGHYELPMLHCADRRQWIVCMVAFPTYEAAEA
                     LKAESRIHRYIFV"
     gene            complement(6122..6307)
                     /locus_tag="VIS73_10980"
     CDS             complement(6122..6307)
                     /locus_tag="VIS73_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY5763718.1"
                     /translation="MLSCKEVTHLLSQEQDRELGFGERLSLRLHLAMCRGCSNFKQQM
                     SFLRAACRRYAGRDERP"
     gene            complement(6301..6903)
                     /locus_tag="VIS73_10985"
     CDS             complement(6301..6903)
                     /locus_tag="VIS73_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5763719.1"
                     /translation="MDERQQSGVLAEGDLAEIRSAMLRFARLQLRDEAAAEDAVQEAL
                     AAALVNADKFAGRASARTWVFSILRNKIVDVIRTQSRSINVSSLMGEGASFDETFDSL
                     FKASEHWQPQARPRDWGDPETSLSEQQFWAVFDACLDHLPEKTARVFMMREFLELESG
                     EICQELAISTDNLYVILHRARNSLRLCLDQSWFVAGEARC"
     gene            complement(6968..7777)
                     /locus_tag="VIS73_10990"
     CDS             complement(6968..7777)
                     /locus_tag="VIS73_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HEY5763720.1"
                     /translation="MNPVARHVGRWLAGWLTRPRVAYTSVSPPTDSRLLAETLQAGDV
                     LLVEGNTRISAAIKYLTQSTWSHAALYVGPQDSAEDPLRALVFIEADTIDGVRCVSLA
                     KYDGLHTRICRPVGLTEEDRGRVVAYALERIGLQYDLKNIFDLARYLFPTPPVPQRWR
                     RRMLTLGSGDPTRAICSTLIAQCFQSVRYPILPQVKIVPAARPGCETCVDEVLTPRHH
                     SLFVPRDFDTSPYFAIVKPTLGEAFDYRRLHWSNDEAEKDLQPSPTAAAGR"
     gene            complement(7886..8569)
                     /locus_tag="VIS73_10995"
     CDS             complement(7886..8569)
                     /locus_tag="VIS73_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY5763721.1"
                     /translation="MTTTRIVVSLLAVVLGLSACATDEFGNSRKMTDAEKGALIGAIG
                     GAVVGGAVTKKKNRKKGVVIGAIGGGLAGAAVGNYMDNQKRDLEKVLMPERNAGAIEI
                     ERLPDNALRVTMTDQTAFAVNSADIQPGFTSTMDKIASVVNRYGKTTITVAGHTDSTG
                     SAEYNQGLSERRAKTVENYFLSKGVVPQRLDSYGFGETSPRSSNSTEAGRMLNRRVEI
                     DIEPVVADQ"
     gene            8785..9336
                     /locus_tag="VIS73_11000"
     CDS             8785..9336
                     /locus_tag="VIS73_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763722.1"
                     /translation="MLNRLPFALLLATASLAAGAEGTRFMPMFTDPDFEFRPTLAAVV
                     GALKPESNPSGLYLGTEVSFNSGVFQTEARRVRSHLTFGHYDQSGLKLTTIDFSPRYT
                     VPMGSGFSIGGGPGIAYLRSTVASRTASHLAWMVAGGVSFRKGGYYAGVDLRYQDTRE
                     KLVGTSPRDADNWGLASKIGYNF"
     gene            complement(9375..10601)
                     /locus_tag="VIS73_11005"
     CDS             complement(9375..10601)
                     /locus_tag="VIS73_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5763723.1"
                     /translation="MTADTAAPRSSRGFDAGTPEYRRANLAMFVAGLACFGMLYGTQP
                     ILPLLAGHFAVSPAAISLSVSAGTFALALALVPASVISDRIGRRTLMRVSLVAAAGFA
                     LFAAFAQSFGQLLLLRALLGLSLAGVPAAALAYLGEEIAPSAQGRAIGLYIAGNALGG
                     MSGRYLSALVTDWADWRIGLATLGVLGAAAAAYFWRALPASRRFVPRHLPFSAVHRDI
                     AGLFADPGLPRLFLVALLLMGAFVGVYNFLGFRLIVPPYDLSPTAIGAIFLLYAVGSW
                     ASAWAGRLADRFGRRNVLWVMVTIAGAGLLLTLAGGLWLIIAGVAVFTFGYFGAHTAA
                     SGWVGRRAGERRALASALYLGGYYIGGSVIGALTGFAWSGYGWPGVVALLALCVGGAL
                     WLALWLRTVPQRQVSG"
     gene            complement(10855..11091)
                     /locus_tag="VIS73_11010"
     CDS             complement(10855..11091)
                     /locus_tag="VIS73_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763724.1"
                     /translation="MKKTMVALAMLAFSGAALAFHCPKDMKQIDAALEKTSLSGEQLA
                     EVKKLRADGEAFHKAGKHQEAVDALAKAMKMLGV"
     gene            11433..11867
                     /locus_tag="VIS73_11015"
     CDS             11433..11867
                     /locus_tag="VIS73_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763725.1"
                     /translation="MATVPPKSLPRWAKRLGYLVFFVAVGALVWTQLPRGAYPTDLGR
                     IGSGRPALVLAYDINSLGGMTVMKLMDELRGEYADRVEFLVADLGVPAGQAFAQRHGA
                     NSGTVVMLSDTGSHLRTLHLPANSQVLREALDAALPQRMGSY"
     gene            complement(11898..>12416)
                     /locus_tag="VIS73_11020"
     CDS             complement(11898..>12416)
                     /locus_tag="VIS73_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5763726.1"
                     /translation="QSIVSRRLDPLDHAVVSVTRFHSGFAHNVIPAKAELAGTARSFR
                     AEVQDQIEQALADVAAGIAASHGVAARVDYRRGYPATHNDASEAARCAAVAGIVAGEA
                     RVTTTLRPSMGAEDFSFFAAQRPSCYAWLGAGPGAGGCTLHSPHFDFNDALLPVGIRY
                     WTALVEQVLPVG"
BASE COUNT         2259 a         4316 c         3899 g         1942 t
ORIGIN      
        1 acgcagcgtc gcgaagaaca ggccctcgcc gccaaacagc gccgacttga tcttgccgac
       61 gtactggata tcgaaattga cgctggtctg gaaggcgacc acacaaccgg tatcgacacg
      121 cagggtttcg ccgggctgga gggtgcgctc gagcagcgtg ccgccagcat gaacgaaggc
      181 gaggccatcg ccctcaagct tctgcatgat gaacccctcg ccaccgaaga ggccgacgcc
      241 aagccgtttc tgcagcgcaa ttccaatcga tacgcccttg gcggcgcaaa ggaaggaatc
      301 cttctggcag atcagcgtgc cgccgatctc ggccagatgc accggaatga tgcgtcccgg
      361 gtagggcgcc gaaaaggcga cgcgcttctt gccctgccct tcgttgtgga aaaccgtggt
      421 gaacagcgac tcaccggtca ggaggcgctt gccggcgccc atcagcttgc cgaagaagcc
      481 tccctgctgc gagccgtcac cgaagacggt gtccatctcg atgccgtcct gcatgtacat
      541 catcgcgccg gcttcgccga ccgccgcctc gccggggtcg agttcgacct cgacgtactg
      601 catgtccgtc ccgaagatct cgtagtcgac cacgtccatc tgcaagctca tttcatccct
      661 ccgcaggaaa accgctaaga ataagtcctg gtgaattgtg cgatcgacgc ctgtggtcgg
      721 caagatcgcc ggtccgtgac aaaatcgagc ctcccgcgca aacgcatctt gccgcgcgca
      781 acctttgccc ggcgattgag tcagaccgca cccgacccat gaaccccgaa acggactcga
      841 tgctcgccga tcgactcagc gcctcgcagc ttgaagccat tcttgagatt gccgacgacg
      901 caatcgtctg cgtcgaccgc aacctccgga tcgcgctgtt caaccgcggc gccgagcgaa
      961 ttttcggcta caaggccaat gaggcgatcg gccagccact caacctgctg atccccgacg
     1021 gggcacgacc gcgacacacc gcgcatgtca ccgacttcca caacagcggc gccagcgccc
     1081 ggcagatggc cgaacgcagc gagatcagcg gtcgccgcaa ggacggcacc accttcccgg
     1141 ccgaggcgac gatttcgcaa ctggccgtcg acggcggggt gatcttcacg gccatcctcc
     1201 gtgatgtcac gctacgcaag gcgcacgagc aggagctcga gaacgcccgt gcggtagcgg
     1261 aggcggcaac ccaggcgaag agcatgttcc tcgccaacat gagccacgag ttgcgaacgc
     1321 ccctcaacgc ggtcgtcggc atgaccagcc tgctccttga cacggccatc accgaagagc
     1381 agcgcgacta cgcgcagacg atccgcgcca gcagcgaaac cctgctggca atcatcaacg
     1441 acatcctcga ctactcgaag atcgagatcg gccgcctcga gattgagagc cagcccttcg
     1501 acctgcggcg cgccatcgag gagtcgctcg acctcgtcgc gccaatcgcc ggcgaaaaga
     1561 atctcaacct cgcctatctg atcgccgaag gcgtacccgc cggcatccag ggcgacgtca
     1621 cccggctgcg gcagattctc gtgaacctgc tcgccaacgc caccaagttc acccaccacg
     1681 gcgaggtgct ggtctcggtc gaaggcagcc cactcgacaa cggccatttc gaactgcact
     1741 tctcggtagc cgacaccggc atcggcatcc cggtcgaccg gatcgaccag ctgttccagt
     1801 ccttcaccca ggtcgacgcc tcgaccacgc gcaagtacgg cggcagcggc cttggactgg
     1861 cgatcagccg ccgcctcgcc gagatgatgg gtggccggat gtgggtcgac agtgtcgccg
     1921 gcgaaggttc gaccttccac ttcaccatcg aggcagcgcc gatcgacgcg cgcagtgcac
     1981 acaacgtatt gcaggagcgt tcggcggcgc tgtcgggcaa gcgcctgctg atcgtcgacg
     2041 acaacaccac caaccgacgc atcctggtca agcaggcgct tctctggggc atgctgccct
     2101 cggcggcggc gacgccggtc gaggcaatcg acctgattcg ccacggccac gccttcgacg
     2161 ccggcattct cgacatgcat atgcccgaca tggacgggct gatgctggcg cgggagattc
     2221 gccgtctgcg tgatgcgaat tcgctgccgc tgatcatgct gacctcgctg gttcagcggc
     2281 cgaagcctgc cgaggccgag caactggcgt tttcggcctt tctcaacaag ccgatcaagc
     2341 cgacagcact gttcgatgcg ttggcggtcg ccttgcgctt gccgcctgcg agtgcaccgg
     2401 cggtatcgac aacgacccgc cgcgtacgtg gcaacggttc gcgcaacctg cgcttgctgg
     2461 tcgccgagga caacaccgtc aaccagaagg tcatgcagca gttgctcgcc cacctcggct
     2521 atcaggccga cgtagcctcg accggcggcg aggcgctcga cgcgctcgag cgccagccct
     2581 acgacgtcgt gctgatggac gtgcagatgc cggagatgga cgggctcgag gcaacccggc
     2641 ggattcgcac gcacttcggt gcctcaccct acatcgtggc gatgaccgcc aacgcgatgg
     2701 tcggcgatcg tgaggagtgc ctcaaggccg gcatgaacga ctacctgccc aagcccgtcg
     2761 agctgacgca actgagcatc gtcctcgacc gcgccgccgc ggccgccggc gaggcgccca
     2821 acgcggtcac gactgacgct gcagaaacgc tagctgcgga cggaactgca cacgaaaccg
     2881 cgccgcggat tatcgatcgg cgccagatcg accagcttcg ctcgctggaa ggcccggaca
     2941 acccgaatct cgtcgccgag atcgtcgaca tgttcctgcg cgatgcgcca cagcacctcg
     3001 acggcctcgc cgccgccgcc gccgcgggcg acgccgccgc actcaaggcc atcgcccatc
     3061 gatttctctc gagcatcgaa aacctcggtg cgatagcgat gcgcgagcgc tgcatgacgc
     3121 tcgaagcact cgggcgggag ggccggagcg acggcgctgc ggaactcgtc gctcgcctgc
     3181 gcgacgactt cgcggcggcg cgcgaggcgc tgctgagcct gctcggcgcc accgcccgat
     3241 gaaacctgac ttcgacgtcg caatcctcgg cggcggcttg gccggcgcca gcctggcgct
     3301 cgcgctgcgc gccagcggcc gccggatcgc cgtcgtcgat gcgcgtccgg caaacgcctc
     3361 ggtagcggcc agcgatccgc gcgcctacgc aatcagcccg acggcgatgg aattcctcga
     3421 aacgatcggc gtctggcggc acctcgatcg gcaatgcgtc ggcccggtgg tagcgatgga
     3481 tatccgcggc gaccgaggcg gccggctgcg attttcggcc ttcgaagcca ggcgcgaagc
     3541 gctggcgtgg atggtcgagg ccgagacgct cgaagccgaa atccgcgaga ccgcacgccg
     3601 acaggccgga atcgactggc tggcaccggc gacgccaacg gcaatgaccc agaacaccga
     3661 cgcggcaacg ctgacgctct cgaccggcgt gcgcctttcg gcccgcctgc tggtcggcgc
     3721 cgacggtcgc gactcgtggc tgcgcgaaac agccggcatc gccgccacga ccaccggcta
     3781 cggcgaacaa ggcatcgccg cctgctacgc ctgcgaacaa gcgcatggcg acactgcccg
     3841 tcagtggttc catggggaca gcgtgctcgc ctggctaccg cttcccgagc agcgcatctc
     3901 gatcgtctgg tcggcacccg actcgctggc cacgcatctc accgcgctcg atcccgaagc
     3961 gctggccctg cgcgtcggcg ccgccggcga ccacgtactc ggccgcctga caccgctggg
     4021 cccggcgcgc ggctttccgc tgcgcctgat ccgcgtgccc gacccgatcg ccagccgcat
     4081 cgcgctgatc ggagacgccg cccacggcat ccacccgctt tcggggcacg gcatcaatct
     4141 cggcttcagc gacgcgcgcg aactcgcggc ggcgatcacc gattgcccgc ccggcggcga
     4201 tgtcggcggc gagcgcgtcc tccgccgcta ccgccgccgc cgcgccgagg aagttcgcct
     4261 tctccaggag gccacgcacg gactgcgcca gctgttcgca tcccgtgcgc cggggctgtc
     4321 gctgctgcgc aatctcggtc tgtcggtcac cgaccgcctg cctcgcgtaa aatccctgct
     4381 tacccgctac gcgatgggct gaggcccgcg ccggcatttc cggagtcatt cgatgtcatt
     4441 tccccgcaac aacccgttcg ccgcggctct ggtcgccctc gccctcgccc tgccgctcgc
     4501 cgcccaggcc aacgacaagg tcagcgccgc cttcgagaag gccttccgcg tcaagcccga
     4561 aaaggtcacc cggatcgagg ccatcggcct ctacgaaatt gtcgtcggcg gcgacatcct
     4621 ctacaccgac gaaaaggtca cccacctgat ccagggcagc ctaatcgacg tgaagacccg
     4681 gaccaacctt accgaggagc ggctgaacaa gctgctggca atcaacttcg ccgagttgcc
     4741 gctcgatctc gcggtcaagc aagtgcgcgg caacggcaag gcgacgctgg tgaccttcga
     4801 agaccccaac tgcggctact gcaagcggct cgcgcgcgac ctgaccaatc tcgaagacac
     4861 gacgatctac accttccttt acccgattct ctcggccgat tcgaccgaga agtcgcgcgc
     4921 gatctggtgc gccaaggacc gcgcccgtgc ctggaacgac tggatgatcg acggcaaggc
     4981 accaaccgct gccacctgcg acgcccctct tgagcaactg gtcgaactgg gccagcgtta
     5041 tcgcgtcacc ggcacgccga cgatcttctt caccgacggc tcgcgcgcac ctggcgccat
     5101 tccgctgcag cagatccgcg agcggctggc caagatcgcc ggcaagtgac cgccatcacc
     5161 gaggaagtag cgtaggcggc gccgctgcgt taaggttcgc tcataaaatg cgcaacaaag
     5221 aaagttcagg gggattgcat gggattactt aacggactct tgggcaggcg caaggcgtcg
     5281 ccgactgccg cccacccgct gctcgaacat gtgccggccg tactcggcga cgagattctc
     5341 gcggcgccag atttcgccga gaccctgatc cccgcgctcg agcacgccga tgcctacctg
     5401 aagaatcagg tagcggcgat tccgggcccc ctgacgctgt ccagcgcaac gcggccgacc
     5461 gacccgctaa tgagccgcat cttcgccgac gacgccgagc ttcgcgccgg gctcggccgc
     5521 agcctcgaga tgaaggagtc gatcgtccag ctagcggtcg ccggacatgt gcacagctac
     5581 gcgctgctcg gcatgcgtct gaagcgtggc gtcacgccgg cgccgggcga agcgccggta
     5641 tttgccgatc acacggtcaa ggcgattgcc gataccgaag cgcatgcccg cggcgaactg
     5701 cgcgtttccg cgctgcgccg gatcatcaag aactacgacc agcatctcgc caagctgcgc
     5761 gaaaagcagc agctgcttaa gctcgagtgg aacatcgaga acaatccggc aggcaaggac
     5821 gacggcaacg aattcgtcca cgccaagacc gagctgacgc cgggcaacat ggtgcgcggc
     5881 ctagccgcct ggctacgccg acccgaggaa gtgctcaagc tcaccaacgg cgatgtacgc
     5941 atcccgggcc gcagcgccga cggggcgcag agcggccatt acgagctgcc gatgctgcat
     6001 tgcgccgacc ggcgccaatg gatcgtctgc atggtcgcct tcccgaccta cgaggccgcc
     6061 gaggcactca aggccgagtc gcggattcac cgctacatct tcgtctgacc cggcccagcc
     6121 ttcaggggcg ctcgtcccgc ccggcgtagc gccggcaggc cgcgcgaagg aagctcatct
     6181 gctgcttgaa gttggaacag ccgcgacaca tcgccaggtg cagtcgcaag gacaggcgtt
     6241 cgccgaatcc cagctcgcga tcctgttcct gcgacaacag atgggtcact tccttgcagc
     6301 tcagcatcgt gcctctcctg cgacaaacca gctctggtcc agacacaagc gcaggctgtt
     6361 gcgcgcccgg tgcaggatta catagaggtt gtcggtactg atcgccagtt cctgacagat
     6421 ctcgccggat tcgagttcga ggaactcgcg catcatgaaa acacgggcgg ttttctccgg
     6481 caaatggtcg aggcaggcgt cgaaaacggc ccagaactgc tgctcggaaa gactcgtctc
     6541 gggatcaccc cagtcgcgcg gacgcgcctg cggctgccag tgttcgctgg ccttgaacag
     6601 cgaatcgaag gtctcatcga aggaggcgcc ctcgcccatc agcgacgaga cgttaatgct
     6661 gcggctctgg gtgcggatca cgtcgacgat cttgttgcgc aggatcgaaa agacccaggt
     6721 ccttgcactg gcgcggccgg cgaacttgtc ggcattgacc agcgccgcgg ccagcgcctc
     6781 ctgcaccgca tcttcggcgg ccgcctcgtc gcgcaattgc aggcgcgcga agcgcagcat
     6841 tgcgctgcga atctccgcga gatcgccttc cgccaataca ccgctctgct gccgctcgtc
     6901 caccgctacc cttttgccat cggccccgac gagccgcact gtatccgctg tcgtcgccgg
     6961 tggccactca ccggccggcg gccgctgttg gcgacggctg cagatccttc tcggcttcgt
     7021 cgttcgacca atgcaggcgc cgataatcga aggcctcgcc caatgtcggc ttgacgatcg
     7081 cgaagtaggg cgaggtgtcg aaatcgcgcg gcacgaaaag gctgtggtgg cgcggggtca
     7141 ggacttcatc gacgcaggtc tcacatccag ggcgggcggc cgggacgatc ttgacctgcg
     7201 gcaggatcgg ataacgaacc gactgaaagc attgtgcgat caaggtcgag cagattgccc
     7261 gcgtcggatc tccgctgccc agcgtcagca tccggcgccg ccagcgctgg ggcaccggcg
     7321 gcgtcggaaa caggtagcgc gccaggtcga agatattctt caggtcgtac tgcaggccga
     7381 ttcgctccag tgcataggca acgactcggc cacggtcttc ctcggtaaga ccaaccgggc
     7441 gacagatccg tgtgtgcaac ccgtcgtact tggcgaggct gacgcaacgc acgccgtcga
     7501 tcgtgtcggc ctcgatgaaa accagcgccc gcagggggtc ttcggcggag tcctgcgggc
     7561 cgacgtagag cgccgcatgc gaccaggtcg actgggtgag gtacttgatc gccgcgctga
     7621 tccgcgtgtt gccctcgacc agcaggacat caccggcttg tagcgtctct gccagcagcc
     7681 tcgaatcggt tggcggcgaa acgctggtat aggccacacg cggccgggtc agccacccag
     7741 ccaaccagcg accgacgtgg cgggctacgg gattcatggc ggccctccga tcgaacaccg
     7801 tacatccatt ggacgcgcgg gcctcgccga tcttacgcgc acgctcggca cggggccgcc
     7861 cctgccgagc gccggacgtt tactgctact ggtcggcgac caccggttcg atgtcgatct
     7921 caacgcgccg gtttagcatg cggcccgctt cggtcgaatt gctcgaacgc ggcgaagtct
     7981 cgccgaagcc atacgagtcg aggcgctgcg gcacaacgcc cttgctcagg aagtagttct
     8041 caacggtctt agcgcggcgc tcggacagtc cctggttgta ctcggcagaa cccgtgctat
     8101 cggtatggcc ggcgacagtt atcgtggtct tgccatagcg attgacgacg ctggcgatct
     8161 tgtccatcgt gctcgtgaag ccgggctgga tgtccgccga gttgaccgcg aaggcggtct
     8221 ggtcggtcat cgtcacgcgc agcgcgttat cgggcagccg ctcgatttca atcgccccgg
     8281 cgttgcgctc gggcatcagc accttttcga gatcgcgttt ctggttgtcc atgtagttgc
     8341 cgactgcggc accggctagg ccgccaccga tcgcgccgat aacgaccccc ttcttgcggt
     8401 ttttcttctt ggtcacggcg ccaccgacaa cggcaccacc gatcgcaccg atcagcgcgc
     8461 ccttttcggc gtcagtcatt ttccgggaat tgccgaattc gtcggtggca caggccgaca
     8521 ggccgagaac gacagcgagc agggatacga cgatgcgtgt tgtagtcatg ggaagcctca
     8581 tgcagatgac aggaaattga tcgaacgggc cgcattatgc ctatgcggcg taccttcgcc
     8641 gcgcgacccc ggttggacaa cgctcatccc gacagttctg ctgaccgaag gagtagagtc
     8701 acgccgattg ccgggaagcc ggccgccggg tgaacgaacg accggcttgt gcggcaccgg
     8761 cgacgcgcgc cacagaggaa actgatgcta aaccgactgc cgtttgcgct cctgctcgcc
     8821 actgccagcc ttgcggcagg ggcagaaggc acccgcttca tgccgatgtt caccgatccc
     8881 gatttcgagt tccggccgac tctcgccgcg gtggtcggag cgctcaagcc ggagagcaac
     8941 ccgtctggcc tctacctggg caccgaagtc agcttcaaca gtggcgtttt ccagaccgag
     9001 gctcgacgcg tgcgaagcca cctcaccttc ggccactacg accagagcgg cctgaaactc
     9061 acaacgatcg atttctcgcc gcgctacacg gtgccgatgg gcagcggctt ctcgatcggc
     9121 ggcggaccgg gcatcgccta cctgcgctca accgtcgcca gtcgcacggc gagccatctt
     9181 gcttggatgg ttgccggcgg cgtcagcttc cgcaagggcg gctactacgc gggcgtcgac
     9241 ctgcgctacc aggacacccg cgagaaactc gtcggcacat caccccgcga tgctgacaac
     9301 tggggcctcg cgagcaagat cggctacaac ttctgatggc gttggccgcc ggtagcggcg
     9361 aacccgcccg taattcaccc gctcacctgt ctctgcggta ccgtacgtaa ccacagcgcc
     9421 agccacagcg caccgccgac gcatagcgcc agtaaggcca ccacgcccgg ccagccgtag
     9481 ccgctccagg caaagccggt cagtgcgccg atgacgctgc caccgatgta gtagccgcct
     9541 aggtacagcg ccgaggccag cgcccgccgc tcgccggcgc ggcgtccgac ccagccgctc
     9601 gccgcggtat gcgcgccgaa gtagccaaag gtgaatacgg cgacgccggc gatgatcaac
     9661 caaagcccgc cggccagtgt cagcagcagg ccggcgccgg cgatcgtcac catcacccag
     9721 agcacgttgc gccgcccgaa acggtctgcg agccgcccgg cccaggccga cgcccaactg
     9781 ccgaccgcgt aaagcaggaa gatcgcgccg attgccgtcg ggctcagatc gtagggcggc
     9841 acgatcaggc gaaagccgag aaaattgtag acgccgacga aggcccccat cagcagtagc
     9901 gctacgagga aaagccgcgg cagcccggga tcggcgaaca ggcccgcgat gtctcgatgc
     9961 acggccgaga acggcaaatg ccgtggcacg aaccggcgcg aagccggcag cgcccgccag
    10021 aagtaagccg ccgccgcggc gccaagaacg cccagtgtcg cgagaccgat tcgccagtcc
    10081 gcccagtcgg tgaccagcgc cgaaaggtag cgcccgctca tgccgccgag tgcgttgcca
    10141 gcgatataga gcccgatcgc ccgcccctgc gccgagggcg cgatctcctc gccgaggtag
    10201 gcaagcgccg ccgccggcac gccggccagc gacaggccca gcagcgcgcg cagcagcagc
    10261 agctggccga agctctgggc aaaggcggca aacagcgcaa agccggccgc cgccaccagc
    10321 gacacgcgca tcagcgtgcg ccgcccgatc cggtcggaaa tcacgctcgc cggcaccagc
    10381 gccagcgcca gcgcgaaagt gccggccgag accgagaggc tgattgccgc cggcgacacg
    10441 gcgaaatggc cggcaagcaa aggcaggatt ggctgcgtgc cgtagagcat gccgaagcag
    10501 gccagcccgg cgacgaacat cgccaggttg gcgcggcggt attcgggtgt cccggcgtcg
    10561 aagccgcggg aggatctagg ggcggctgta tcggccgtca tgggcgggac ccgaagcggt
    10621 gggttggcag gcgcccatcg taaatggcgc aggccggggc gtccatgtac ggcgccggcc
    10681 gagacgcttg cggaaacggg gacggcggac tcccgcgcta tgtccccgcg cccgcgccgg
    10741 cgccggcgcc gaagagttct ttcaggcgga ggccggaagc agcgaatggg ctcgcccctg
    10801 cggcaacgaa aacggcccgc gcaagcgggc cgttcgtgga cacaagaccg ggcttcagac
    10861 acccagcatc ttcatcgcct tggccagcgc atcgacggct tcctgatgct tgccagcctt
    10921 gtggaaggcc tcgccgtcgg cgcgcaattt cttcacctcg gccagttgtt cgccgctcag
    10981 gctggtcttc tccagcgccg cgtcgatctg cttcatgtcc ttcgggcagt gaaaggccaa
    11041 cgccgccccc gaaaatgcca gcatcgccag tgccaccatc gtcttcttca tcttcattct
    11101 cctaacggaa tgcgatcgcg taccacggca ctccaacggg cagcgtcagc ctggcaccga
    11161 tcgcaaatgc cggcaggccg ctgccttctg cgtcaacagc acaggcattg tctttgttcc
    11221 atcgggacga cggcatgttc cgaagcaccc ggcgctggca gcgggtcccg tgccctgcgg
    11281 agctagtggc aggaatcggc ttgctcgaat caagcagtcg tgccagcgcg gtcagccgat
    11341 ggcctttcgc cgatcgcgtt ccgccctggg cactagaacc ggcccgccgc tgtctatggg
    11401 aatcaaccca tctgaccgaa ggactccgat caatggcaac tgtcccaccg aaatcccttc
    11461 cgcgctgggc gaaacggctc ggctatctgg tctttttcgt cgcggtcggc gccttagtct
    11521 ggacccagct gccgcgtggc gcctacccga ccgacctcgg ccgaatcggc tccggacgcc
    11581 cggccttggt actcgcctac gacatcaact cgttgggcgg catgacggtg atgaaactga
    11641 tggacgagct gcgaggcgag tacgccgacc gcgtcgagtt tctggtcgcc gacctaggcg
    11701 tccccgccgg gcaggcgttc gcccagcgcc acggagcgaa cagtggcacg gtggtcatgc
    11761 tctccgatac cggttcgcac ctccgcacac tgcacctgcc ggcgaactcg caggtactgc
    11821 gcgaggcgct cgacgccgcg ctgccgcagc ggatgggtag ttactgagga gcggaggccg
    11881 gttcagtctg cgcccgatca acccaccggc agaacctgct ccaccaatgc cgtccagtag
    11941 cggataccga ccggcagcag tgcgtcgttg aagtcgaagt gcgggctgtg cagcgtgcag
    12001 ccgccggcgc cggggccggc gccgagccac gcgtagcagc ttgggcgctg ggcggcgaaa
    12061 aaggagaagt cctcggcgcc catcgacggg cgtagcgtgg ttgtcacgcg cgcctcgccg
    12121 gcgacgattc ccgcgacggc ggcgcagcgc gccgcctcgc tcgcatcgtt gtgcgtggcc
    12181 gggtagccgc gacggtagtc aacgcgggcg gcgacgccgt gactggcggc aatgccggcg
    12241 gcgacgtcgg cgagtgcctg ctcaatctgg tcctgcactt cggcgcggaa gctgcgcgcg
    12301 gtaccggcta gctcggcctt ggccggaatg acgttgtggg cgaaccccga gtggaagcgc
    12361 gtgacactga cgacggcatg gtcgagcgga tcgagccggc ggctgacgat gctctg
//
LOCUS       DATDAA010000368        10795 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_270941,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000368 DATDAA010000000
VERSION     DATDAA010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10795
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270941"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3180
                     /gene="rpoB"
                     /locus_tag="VIS73_11025"
     CDS             <1..3180
                     /gene="rpoB"
                     /locus_tag="VIS73_11025"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY5763727.1"
                     /translation="AIYRMMRPGEPPTEEAVEILFKGLFYADERYDISGVGRMKFNRR
                     LGRGADLEYKVVLTGASRAEASIKALANVLQVPPPMMQEMVADLHFGARSIVENLSRE
                     EAESFVASLKQAGAQVEVREQMTLSPRDIVDVIKILVELRNGRGEIDDIDHLGNRRVR
                     SVGELAENQFRAGLVRVERAVKERLSQAESDNLMPHDLINAKPISAAIKEFFGSSQLS
                     QFMDQTNPLSEITHKRRVSALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEGPNIG
                     LINSLALFARTNDYGFLETPYRKVVDGRVTEHIDYLSAIEEGHFMIAQANAEVEEKTG
                     RLTGELISCRNKGEFMLATRDRVEYMDVAPSQIVSVAASLIPFLEHDDANRALMGSNM
                     QRQAVPCLRPEKPLVGTGIERTVAVDSGTAVIALRGGTVDYVDAARIVVRVNDDEAVA
                     GEVGVDIYNLVKYTRSNQNTNINQRPMVKVGDQIAMGDVIADGASTDKGELALGQNML
                     VAFMPWNGYNFEDSILISEKVVAEDRYTSIHIEELTVVARDTKLGPEEITRDIPSLGE
                     AQLARLDESGIVYVGAEVLAGDVLVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDTS
                     LRVPAGIAGTVIDVQVFTREGIERDARAASIIDEMLRHYKQDLGDQMRIVERDTFARA
                     QRLLKGKIANSGPKKIARGTALTDEYLTSLDPHQWFDLRLADDETAGQMEQIRDGLEK
                     LRKDFDLAFEEKKKKLTQGDELPPGVQKMVKVYVAVKRRLQPGDKMAGRHGNKGVVSK
                     INPIEDMPYMADGSPVDVVLNPLGVPSRMNVGQILEVHLGWAGKGIGQKIDRMLRQQA
                     AVADVRKYVEQVYNSAGRAADIKSLNNGELLELADNLRAGVPFATPVFDGATEEDIKA
                     MLRLAEMPESGQVTLFDGRTGDAFERPVTVGYMHVLKLHHLVDDKMHARSTGPYSLVT
                     QQPLGGKAQFGGQRFGEMEVWALEAYGAAYVLQEMLTVKSDDVNGRTKVYENIVKGDH
                     KIDAGMPESFNVLVKEIRSLAIDIDLQRY"
     gene            3205..7425
                     /gene="rpoC"
                     /locus_tag="VIS73_11030"
     CDS             3205..7425
                     /gene="rpoC"
                     /locus_tag="VIS73_11030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY5763728.1"
                     /translation="MKALLDLFRQVTPEEEFDAITVGLASPDKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTLAKVRRERM
                     AHIDLASPTAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAYVVTDPGVTPLNRAQLL
                     SEDDYLAKVEEYGDEFKAVMGAEGVRDLLCSLQIDYEIDRMNAELETSGSETKNKKLG
                     KRLKVLEAFKKSGIKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIKGK
                     GGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKLMALELFKPFIFNRLEMMGLAT
                     TIKQAKKMVESQEPVVWDILEEVIREHPVMLNRAPTLHRLGIQAFEPTLIEGKAIQLH
                     PLVCAAFNADFDGDQMAVHVPLSIEAQMEARTLMLASNNVLSPANGTPIIVPSQDIVL
                     GLYYATRDKINGKGEGMVFADVSEVYRALAAGQVETHSRVSVRIREVMVAEDGSHSEK
                     ITRYQTTVGRSMLSEILPPGLPFKAIDKPLKKKEISKLIDESFRRCGLKDTVVFADKL
                     MQNGYALATRAGISFCADDMRVPTQKTAILAAAKDEVREIEAQYTNGLVTQGERYNKV
                     VDIWGRAGDQVAKVMMEQLGHEDTIDRHGKKVKQESFNSIYMMADSGARGSAAQIKQL
                     AGMRGLMAKPDGSIIETPIYSNFREGLNVLEYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVTQDLVITEDDCGTSNGFVMKALIEGGEVIEPLRERILGRVTADDVLDPETQNI
                     LFPAGTLIDEDACDTIDRLGVDEVKARTPITCDTRWGLCAKCYGRDLGRGSPVNVGEA
                     VGVIAAQSIGEPGTQLTMRTFHVGGAASRSAAASQVEAKSAGVVRFTSAVRYVSSAKG
                     DKVVISRSGEVLVTDDNGRERERHKVPYGAVLQVGDGQTIKAGVKLATWDPHTRPIVT
                     EYAGTVKFENVEEGRTVAKQIDDVTGLATLVVIDPKRGTKAQVKGVRPQVKLIDPVTE
                     EEVRIAGTEHSVAITFPIGSIITVADGQTVGVGDILARIPQESAKTRDITGGLPRVAE
                     LFEARAPKDIGMLAEFTGTVSFGKDTKGKQRLVITDLDGTTHESLIPKEKHVLVHDGQ
                     VVNKGELIVDGEADPHDILKMKGVEALARFITDEVQDVYRLQGVKINDKHIEVIVRQM
                     LRRVVITDPGDTRFIKSEQTERSEILDENDRMAEKGKRPALFEPMLLGITKASLSTDS
                     FISAASFQETTRVLTEAAIMGKRDELRGLKENVIVGRLIPAGTGLAYHNTRRAQKSGE
                     DLAAERGLFENMPTATAEEGGDAPVDAA"
     gene            7553..7930
                     /gene="rpsL"
                     /locus_tag="VIS73_11035"
     CDS             7553..7930
                     /gene="rpsL"
                     /locus_tag="VIS73_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY5763729.1"
                     /translation="MPTINQLVRKPRTAIKAKSKVPALNACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTQGVKDRKQSRSKYGAKRPKAA"
     gene            8027..8497
                     /gene="rpsG"
                     /locus_tag="VIS73_11040"
     CDS             8027..8497
                     /gene="rpsG"
                     /locus_tag="VIS73_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002006497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY5763730.1"
                     /translation="MPRRREVPKREILPDPKFGSVDLSKFVNVLMESGKKSVAERIIY
                     GAFDQITNKAGKDPLEVFGLALNNVKPLVEVKSRRVGGANYQVPVEVRPARRMALAMR
                     WLREAARKRGERSMGQRLAGELLEAAENRGGAVKKRDEVHRMAEANKAFSHYRF"
     gene            8536..10635
                     /gene="fusA"
                     /locus_tag="VIS73_11045"
     CDS             8536..10635
                     /gene="fusA"
                     /locus_tag="VIS73_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY5763731.1"
                     /translation="MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMAQEQERGITITSAATTCFWKGMGQDFPEHRINIIDTPGHVDFTIEVERS
                     MRVLDGACMVYCAVGGVQPQSETVWRQANKYKVPRLAFVNKMDRQGANFFKVVEQMKT
                     RLKANPIPIVIPIGAEENFEGVVDLIKMKAIYWDQESQGMKFDFRDIPAGLADKAQEW
                     REQMLEAAAEASEELMNKYLEEGDLSEADVLAGLRKRTIACEIQPMLCGTAFKNKGVQ
                     RMLDSVIELMPSPIDIPPVVGSDPDDAEKADSRAASDEAPFSALAFKIMTDPFVGQLT
                     FIRVYSGVLNSGDTIINPIKGRKERIGRLLQMHANNREEIKEVRAGDIAAVVGLKEAT
                     TGDTLCDMAKPIVLVRMEFPEPVIHVAVEPKTKADQEKMGLALNRLAQEDPSFRVRTD
                     EESGQTIISGMGELHLEILVDRMKREFNVEANVGAPQVAYRECIKKAVEQEGKFVKQS
                     GGRGQYGHVWIKIEPNEAGKGYEFVDAIKGGTVPREYIPAVDKGLQDTLPNGVLAGFP
                     VVDVKVTLFDGSYHDVDSNENAFKMAASMAFKDGMRKASPALLEPMMAVEVETPEDFM
                     GNVMGDLSSRRGMVQGMEDLPGGIKAVKAEVPLAEMFGYSTTLRSLTQGRATYSMEFK
                     HYAEAPKNVAEAVINKK"
     gene            10656..>10795
                     /locus_tag="VIS73_11050"
     CDS             10656..>10795
                     /locus_tag="VIS73_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY5763732.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITSILSKKFGGEA
                     KD"
BASE COUNT         2257 a         3323 c         3405 g         1810 t
ORIGIN      
        1 gcgatctatc gcatgatgcg tcccggcgag ccgccgaccg aggaagccgt cgagatcctc
       61 ttcaagggcc tgttctacgc tgacgagcgt tacgacattt ccggcgtcgg ccggatgaag
      121 ttcaaccgcc gtctcggccg cggcgccgac ctcgaataca aggtcgtgct gaccggcgcc
      181 agccgggcgg aagcctcgat caaggcgctg gccaacgtgc tgcaagtgcc gccgccgatg
      241 atgcaggaaa tggttgccga tctgcatttc ggtgcgcgtt cgattgtcga aaacctgtcg
      301 cgcgaagagg ccgagagctt cgtcgcctcg ctcaagcagg ccggcgccca ggtcgaggtg
      361 cgcgagcaga tgacgctcag cccacgtgac atcgtcgacg tgattaagat tctcgtcgag
      421 ctgcgcaacg gtcgtggcga aatcgacgac atcgaccacc tcggcaaccg ccgcgttcgg
      481 tcggttggtg aactggcaga aaaccagttc cgcgctggac tcgtccgcgt cgagcgcgcg
      541 gtcaaggagc gcttgagcca ggccgagtcc gacaacctga tgccgcacga cctgatcaat
      601 gccaagccga tcagcgctgc aatcaaggag ttcttcggtt cctcgcagct gtcgcagttc
      661 atggaccaga ccaatccgct gtcggagatc acccacaagc gtcgcgtctc ggccctcggc
      721 cctggcggtc tgacccgcga acgcgccggc ttcgaggtgc gcgacgtgca cccgacccac
      781 tacggtcgtg tctgcccgat cgagacgccg gaaggcccca acatcggcct gatcaactcg
      841 ctggcgctgt ttgcgcgcac caacgattac ggcttcctcg agacgcctta ccgcaaggtc
      901 gtcgacggac gcgttacaga acacatcgac tacctgtcgg cgatcgagga aggccacttc
      961 atgatcgccc aggccaacgc cgaggtcgaa gagaagaccg gccgcctgac cggcgagctg
     1021 atctcctgcc ggaacaaggg cgaattcatg ctcgccacgc gcgatcgtgt cgaatacatg
     1081 gacgtcgcgc cgtcgcagat cgtttcggtc gccgcctcgc tgatcccgtt cctcgagcac
     1141 gacgacgcca accgcgcgct gatgggttcg aacatgcagc gtcaggcagt cccctgcctg
     1201 cgcccggaga agccgctggt cggcaccggc atcgagcgga ccgttgccgt cgactcgggc
     1261 accgcggtca tcgcgctgcg cggcggtacg gttgattacg tcgatgcggc ccgtatcgtg
     1321 gtccgcgtca atgacgatga ggccgtcgcc ggcgaagtcg gtgtcgacat ctacaacctc
     1381 gtcaagtaca cccgctcgaa ccagaacacc aacatcaacc agcggccgat ggtcaaggtc
     1441 ggcgaccaga tcgcgatggg cgatgtcatc gccgatggcg cctctaccga taagggcgaa
     1501 ctggcgctgg gacagaacat gctggtcgcg ttcatgccct ggaacggcta caacttcgag
     1561 gactcgatcc tgatttcgga gaaggtcgtt gccgaggacc gttacacctc gattcacatc
     1621 gaggagctga cggtggtggc gcgcgatacc aagctcgggc ccgaggaaat cacccgcgat
     1681 atcccctcgc tgggcgaggc gcagctggcg cgtcttgacg aatcgggcat cgtctacgtc
     1741 ggcgccgagg tgctggctgg tgacgtgctg gtcggcaagg tgacccccaa gggcgagact
     1801 cagctaaccc ccgaagagaa actgctgcgc gcgatcttcg gcgagaaggc ctccgatgtg
     1861 aaggacacct cgctgcgcgt gccggctgga atcgctggca cggtcatcga tgtccaggta
     1921 ttcacccgcg aaggcatcga gcgcgacgcg cgggccgcgt cgatcatcga cgagatgttg
     1981 cgccactaca agcaggatct cggcgaccag atgcgcatcg tcgaacgcga cacctttgct
     2041 cgtgcccagc gcctgcttaa gggcaagatc gccaactccg ggccgaagaa gatcgcgcgt
     2101 ggtacggcgc tgaccgatga gtacctgact tcgctcgacc cgcaccagtg gttcgatctg
     2161 cgtctggccg acgacgaaac ggcggggcag atggaacaga tccgcgacgg tctcgagaag
     2221 ctgcgcaagg atttcgacct cgcctttgag gagaagaaga agaaactcac gcagggcgac
     2281 gaactgccgc caggcgtgca gaagatggtc aaggtctatg tggccgtcaa gcgccgcctg
     2341 cagccaggcg acaagatggc cggccgtcac ggcaacaagg gtgtcgtctc gaagatcaat
     2401 ccgatcgagg acatgcccta catggccgat ggcagcccgg tcgacgtcgt cctcaacccg
     2461 ctgggcgttc cctcgcggat gaacgtcggt cagattctcg aagttcacct cggctgggcg
     2521 ggcaagggga ttggccagaa gatcgaccgc atgctgcgtc agcaggccgc cgtcgccgac
     2581 gtgcgcaagt acgtcgagca ggtctacaac agcgccggcc gcgctgccga catcaagtcg
     2641 ctgaacaacg gcgaactgct cgagcttgcc gacaacttgc gtgccggtgt gcccttcgcg
     2701 acgccggtct tcgacggtgc gaccgaagaa gacatcaagg cgatgctcag gcttgccgag
     2761 atgcccgagt cgggacaggt gacgctgttc gacggccgca ccggcgacgc ctttgagcgt
     2821 ccggtgaccg tcggctacat gcacgtgctg aagctgcacc acctcgtcga cgacaagatg
     2881 cacgcacgtt cgaccggccc gtactcgctg gtcacgcagc agccgctcgg cggcaaggca
     2941 cagttcggcg gccagcgctt cggcgagatg gaagtctggg cgctagaggc ctacggcgcg
     3001 gcctacgtcc tgcaggagat gttgaccgtc aagtccgacg acgtgaacgg ccggaccaag
     3061 gtctacgaaa acatcgtcaa gggcgaccac aagatcgatg ccggcatgcc ggagtcgttc
     3121 aacgtgctgg tcaaggaaat ccggtcgctg gcgatcgaca tcgatcttca gcgctactaa
     3181 tgcattaaca ggagcacagc gaacatgaaa gcactgctcg accttttcag gcaggtcacc
     3241 ccggaagagg agttcgacgc gatcaccgtc ggtctcgcct caccggacaa gatccgctcg
     3301 tggtcttacg gcgaagtcaa gaagccggag acgatcaact accgcacctt taagcccgag
     3361 cgcgatggcc tgttctgcgc caagatcttc ggcccggtca aggactacga gtgtctttgc
     3421 ggcaagtaca agcgcctcaa gcatcgcggc gtaatctgcg agaagtgcgg cgtcgaagtg
     3481 acgctggcca aggttcgccg cgagcgtatg gcgcatatcg atctcgcctc gccgaccgcg
     3541 cacatatggt tcctcaagtc actgccgagc cgcctcggca tggtccttga catgacgctg
     3601 cgcgacatcg agcgcgtgct gtatttcgaa gcctatgtgg tgaccgatcc cggtgtgacg
     3661 ccgctcaacc gtgcgcagct gctgtcggaa gacgactatc tggcgaaggt cgaagagtac
     3721 ggtgacgagt tcaaggcggt gatgggcgcc gagggcgtcc gtgacctgct ctgctcgctg
     3781 cagatcgatt acgagatcga ccggatgaat gccgaactcg agacctcggg ttcggaaacc
     3841 aagaacaaga aactgggcaa gcgcctgaag gtccttgagg ccttcaagaa gtcgggcatc
     3901 aagccggaat ggatgatcct gaccgtgctg ccggtgctgc cgccggacct ccgtccgctg
     3961 gtgccactcg acggcggccg cttcgcgacc tccgacctta acgacctcta ccggcgcgtc
     4021 atcaaccgca acaaccgcct caagcgactg ctcgaactca aggcgcccga gatcatcgtc
     4081 cgcaacgaga agcgcatgct gcaggaggcc gttgactcgc tgctcgacaa cggccgtcgc
     4141 ggcaaggcga tgaccggcgc aaacaaacgt ccgctcaagt cgctcgccga catgatcaag
     4201 ggcaagggcg gccgcttccg tcagaacctg ctcggcaagc gcgtcgacta ttcggggcgt
     4261 tcggtaatcg tcgtcggccc gcagctcaag cttcaccagt gcggcctgcc caagctgatg
     4321 gcgctcgagc tgttcaagcc cttcatcttc aaccgtctgg aaatgatggg tctggcgacc
     4381 acgatcaagc aggcgaagaa gatggtcgaa tcgcaggagc cggtggtttg ggacatcctc
     4441 gaagaggtga tccgcgagca cccggtgatg ctgaaccggg cgccgacgct tcaccgtctg
     4501 ggcattcagg ccttcgagcc gacgctgatc gaaggcaagg cgatccaact gcacccgctg
     4561 gtctgtgcgg cgttcaacgc cgacttcgac ggcgaccaga tggctgttca cgtacccctg
     4621 tcgatcgagg cgcagatgga agcgcggacg ctgatgctgg cgtcgaacaa cgtgctgtcg
     4681 ccggctaacg gcacgccgat catcgtcccc tcgcaggaca tcgttctcgg cctctactac
     4741 gcaacccgcg acaagatcaa cggcaagggc gaaggcatgg ttttcgccga cgtctccgag
     4801 gtctatcgcg cgctggccgc aggccaggtc gaaacccatt cgcgcgtctc ggtgcggatc
     4861 cgtgaagtga tggttgccga agacggcagc cacagcgaga agatcacgcg ctaccagacg
     4921 accgtcggcc gctcgatgct ttcggaaatt ctgccacccg gactgccctt caaggccatc
     4981 gacaagccgc tgaagaagaa agaaatctcc aagctgatcg acgagtcgtt ccgtcgttgc
     5041 ggacttaagg acactgttgt tttcgccgac aagctgatgc agaacggcta cgcgctcgcg
     5101 acccgtgccg gtatctcctt ttgtgccgac gacatgcgcg tgccgacgca gaagaccgcc
     5161 atcctcgccg cggcgaagga cgaggtccgc gagatcgagg cgcagtacac caacggtctg
     5221 gttacccagg gcgagcgcta caacaaggtc gtcgacatct ggggccgagc cggcgaccag
     5281 gtcgccaagg tcatgatgga acaactcggt cacgaggaca ccatcgaccg tcacggcaag
     5341 aaggtaaagc aggaatcctt caattcgatc tacatgatgg cggactcggg cgcccgaggt
     5401 tccgcggcgc agatcaagca gctggccggc atgcggggcc tgatggccaa gcctgacggc
     5461 tcgatcatcg agacgccgat ttattcgaac ttccgtgaag gcctcaacgt tcttgagtac
     5521 ttcatctcga cccacggcgc ccgcaagggt ctcgccgaca cggcgctgaa gaccgccaac
     5581 tccggttacc tgacccgtcg tctggtcgac gtgacccaag atctggtgat taccgaggac
     5641 gattgcggca cctcaaacgg cttcgtgatg aaggcgctga tcgagggagg cgaagtcatc
     5701 gagccgttgc gcgagcgcat cctcggtcgc gtgaccgctg atgacgtgct cgatcccgag
     5761 acgcagaaca tcctcttccc ggcgggaacg ctgatcgacg aggacgcctg cgacacgatt
     5821 gaccgtctcg gcgtcgatga agtcaaggcg cggacgccga tcacctgcga cacccgctgg
     5881 ggtctgtgcg ccaagtgcta tggccgcgat ctcggacgcg gctcgccggt caacgtcggc
     5941 gaggcggtcg gtgtgatcgc cgcgcaatca atcggcgaac ccggcaccca gctgacgatg
     6001 cggaccttcc acgtcggtgg cgcggcatcg cgttcagcgg ctgcaagcca ggtcgaagcc
     6061 aagagcgccg gcgtcgtccg tttcacctcg gcagttcgct atgtcagcag tgccaagggc
     6121 gacaaggtcg tcatctcgcg ctcgggtgaa gtgctggtga ccgacgacaa cggtcgtgag
     6181 cgcgagcggc acaaggttcc gtacggcgca gtgctccagg tcggcgacgg ccagacgatc
     6241 aaggccggcg tcaagctggc gacctgggat ccgcataccc gcccgatcgt caccgaatac
     6301 gccggaaccg tgaagttcga gaatgtcgag gaaggccgca ccgtcgccaa gcagatcgac
     6361 gatgtgacag gcctggcgac gctggtggtc atcgacccga aacgcggcac caaggcgcag
     6421 gtcaagggtg tacgcccgca ggtcaagctg atcgacccgg tgaccgaaga ggaagtccgc
     6481 attgccggaa cggaacactc ggtggcaatt accttcccga tcggctcgat catcaccgtt
     6541 gccgacggtc agacggtcgg cgtcggcgac attctggcgc gcatcccgca ggaatcggcc
     6601 aagacccgcg acatcaccgg tggcctgccg cgcgttgccg agctgttcga ggcgcgcgca
     6661 cccaaggata tcggcatgct tgccgagttc accggcaccg tcagtttcgg aaaggacacc
     6721 aagggcaagc agcggctggt gatcaccgat ctcgacggaa ccacccatga aagcctgatc
     6781 cccaaggaaa agcatgtgct cgtccacgac ggacaggtgg tcaacaaggg tgagctgatc
     6841 gtcgacggcg aagcggatcc gcacgacatc ctgaagatga agggcgtcga ggcgctggcc
     6901 cgtttcatca ccgacgaggt gcaggacgtc taccgcctgc agggcgtaaa gatcaacgac
     6961 aagcacatcg aggtgatcgt tcgccagatg ttgcgccgcg tggtcattac cgatccgggc
     7021 gacacgcgct tcatcaagag cgagcagacc gaacgttcgg agattctcga cgaaaatgat
     7081 cgcatggccg agaagggcaa gcgtccggcg ctcttcgagc cgatgctgct cggcatcacc
     7141 aaggcctcgt tgtcgaccga ttcgttcatc tccgcggcgt ccttccagga aaccacccgc
     7201 gtgctgaccg aagcggcaat catgggcaag cgtgacgaac tccgcggatt gaaggaaaac
     7261 gtcatcgtcg gacgcctgat tccggccgga acgggtctcg cgtaccacaa cacgcggcgc
     7321 gcccagaaat cgggcgagga tctggcggcc gaacgtggat tgttcgagaa catgccgacg
     7381 gcgacagctg aagaaggtgg cgacgcgccg gtcgatgcgg cttgacgcag tgacgggcga
     7441 gcactagaat ctcgcctctt tttgcggcgg tggccgggtc aggccgtgcc gcaggacaac
     7501 gaaccggtcg aggcggctag tccgcctcgg cgtatttttg ggaattttgg acatgccgac
     7561 tatcaatcag ctggtgcgca agccgcgaac cgcaatcaag gccaagagca aggttccggc
     7621 gctcaatgcc tgcccgcaga agcgcggcgt ttgcacgcgt gtttacacga cgacgccgaa
     7681 gaagccgaac tcggcactgc ggaaggtctg caaggtgcga ctgaccaatg gtttcgaggt
     7741 catctcgtac atcggcggtg aaggtcacaa cctgcaggag cactcggtcg tcctgatccg
     7801 cggtggtcgt gtcaaggatt tgccgggcgt gcgttaccac accgtccgtg gctcgctgga
     7861 tacccagggt gtcaaggacc gcaagcaatc ccgctcgaag tacggcgcga agcgtccgaa
     7921 ggctgcctga catcggtcag gtggtttaag taagtagccg ctcattaggg gcggctgtgg
     7981 ggcgccaatc ggcgccccga ctgaatgttg aactgaaagg tagagcatgc cccgtcgtcg
     8041 cgaagtaccc aaacgcgaga tcctccccga tccgaaattc ggcagtgtcg atctctccaa
     8101 attcgtaaac gtcctgatgg aaagcggcaa gaaatcggtc gccgaacgca tcatctacgg
     8161 cgctttcgat cagatcacga acaaggctgg caaggatccg ctggaagttt tcggcctggc
     8221 cctgaacaac gtcaagccgc tcgtcgaagt caagagccgt cgtgttggcg gtgccaacta
     8281 tcaggttccg gtcgaagtgc gcccggcccg ccgcatggcg cttgccatgc gctggctgcg
     8341 cgaagcggcg cgcaagcgcg gcgagcgttc gatgggccag cgccttgcgg gcgaactcct
     8401 tgaagctgcg gagaaccgtg gtggtgcggt caagaagcgc gacgaagtgc accgcatggc
     8461 cgaagcgaac aaggccttct cgcactatcg cttctgagca tcaccagcga cctgaactca
     8521 tagcaaggta atcacgtggc acgtaaaact cccatcgagc ggtaccgcaa catcggtatc
     8581 tccgcgcaca tcgacgccgg caagacgacg acgaccgaac gaatcctttt ttataccggt
     8641 gtcaatcaca agatcggtga agttcatgat ggcgcggcca ccatggactg gatggcgcag
     8701 gagcaggagc gcggcatcac cattacgtcg gcggcgacca cctgcttctg gaagggcatg
     8761 gggcaggatt tccccgagca ccgcatcaac atcattgaca cgccggggca cgtcgacttc
     8821 accattgagg tcgagcgctc gatgcgcgtg ctcgacgggg cgtgcatggt ctattgcgcg
     8881 gttggtggcg ttcagccgca gtccgagacc gtctggcgtc aggccaacaa gtacaaggtg
     8941 ccccgcctgg ccttcgtgaa caaaatggac cgtcagggtg ccaatttctt caaggtcgtc
     9001 gagcagatga agacgcgcct gaaggcgaac cccattccca tcgtcattcc catcggtgcc
     9061 gaagagaact tcgagggggt tgtcgacctg atcaagatga aggcgatcta ctgggatcaa
     9121 gagtcgcagg gaatgaagtt cgacttccgc gacatcccag ccggcctcgc cgacaaggcg
     9181 caggagtggc gcgagcagat gctcgaggcc gccgccgagg cgtccgagga actgatgaac
     9241 aagtacctcg aggaaggcga cctgtcggaa gccgacgtgc tcgccggact gcgcaaacgc
     9301 acgatcgcct gtgaaatcca gccgatgctc tgcggtacag ccttcaagaa caagggcgtg
     9361 cagcggatgc tcgactcggt gatcgagctg atgccttcgc cgatcgatat tccccccgtg
     9421 gtcggctcgg acccggatga cgcggagaag gccgacagcc gcgccgcgag cgacgaagcg
     9481 ccgttctcgg cgctggcatt caagatcatg accgacccct tcgtcggaca attgaccttc
     9541 atccgtgtct actccggcgt gctcaactcg ggcgacacga tcatcaaccc gatcaagggt
     9601 cgcaaagaac ggatcgggcg cctgctgcag atgcacgcga acaaccgcga ggaaatcaag
     9661 gaagtccgcg ccggcgatat cgctgccgtg gttggtctga aagaggcgac gaccggcgac
     9721 accctctgcg atatggccaa gccaatcgtt ctggtgcgca tggagttccc ggagccggtg
     9781 attcacgtcg ccgtcgagcc gaagaccaag gccgaccagg agaagatggg cctcgcgctc
     9841 aaccgcttgg cccaggaaga cccctcgttc cgcgtgcgta ccgacgaaga gtcgggccag
     9901 acgatcatct ccggcatggg tgaactgcac ctcgagattc tcgtcgatcg catgaaacgc
     9961 gaattcaacg tcgaggccaa tgtcggtgcg ccgcaggtcg cctaccgtga atgcatcaag
    10021 aaggcggtcg agcaggaagg caagttcgtc aagcagtcgg gcggccgtgg ccagtacggc
    10081 cacgtctgga tcaagatcga gccgaacgag gccggcaagg gctacgagtt cgtcgatgcg
    10141 atcaagggcg gcacggttcc gcgcgaatac atccccgccg tcgacaaggg cctgcaggac
    10201 acgctgccaa acggcgtgct cgccggcttc ccggtggtcg atgtcaaggt cacgttgttc
    10261 gacggttcgt accacgacgt cgactcgaac gaaaacgcct tcaagatggc cgcctcgatg
    10321 gccttcaagg acggaatgcg caaggccagc ccggcgctgc tcgagccgat gatggcagtc
    10381 gaggtcgaga ccccggaaga cttcatgggc aacgtcatgg gcgacctctc gtcgcgccgc
    10441 ggcatggtcc agggcatgga agacctgcct ggcggcatca aggcggtcaa ggccgaggtt
    10501 ccgctcgccg agatgttcgg ttactcgacc acgctgcgtt cgctgacgca gggccgggcg
    10561 acctattcga tggaattcaa acactacgct gaagcgccga agaacgtcgc tgaagccgtc
    10621 atcaacaaga agtaagcaga aaggaccaga agaaaatggc caagggaaag tttgagcgta
    10681 cgaagccgca cgtgaacgtg gggacgattg ggcacgttga ccatggcaag accacgttga
    10741 cggcggcgat cacgtcgatt ctgtcgaaga agtttggtgg cgaggcgaag gacta
//
LOCUS       DATDAA010000369         5715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_166902,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000369 DATDAA010000000
VERSION     DATDAA010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166902"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VIS73_11055"
     CDS             <1..654
                     /locus_tag="VIS73_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY5763733.1"
                     /translation="GLTGQQPESADGKSMEPLMAHGIQAMSIGFMIDVDTPMVWRGPM
                     VTQALEQLLTQTNWNDLDYLIVDMPPGTGDTQLTLAQKVPVTGAVIVTTPQDIALIDA
                     RKGLKMFEKVGIPILGIVENMSIHICSKCGHAEHIFGEGGATRMCQDYDVELLGALPL
                     EMSIREMADSGKPTVVGAPDSKAAEIYRAIARLVAIRVAEKARDMSSKFPNIVVQHS"
     gene            674..1585
                     /locus_tag="VIS73_11060"
     CDS             674..1585
                     /locus_tag="VIS73_11060"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY5763734.1"
                     /translation="MDWLAEVWNFPLLKTGDGDLLLGRLLGLLLIVVGAWWGAVVIER
                     MIKRVARNARAGAGSQAAIFTWARIARYLVWIVATMVGLQYLGVDLASFALIGGAVGV
                     GIGFGLQNIFSNFISGVILLLEKTLKVGDFVDLESGVRGHVREISLRFTRISTNDNVD
                     VVVPNSEFVNGRVVNWTLDDRLRRTHVAFGVAYGTDKDAVREAALKAARRVESTFEDD
                     TRKAEVWLVGMGESSLDFELVVWVGADFVARPASIEAKYLWAIHDELEQAGISIPFPQ
                     RDIHFDPQGLTVRLDRGRGSNSGTGGA"
     gene            1649..2218
                     /gene="dcd"
                     /locus_tag="VIS73_11065"
     CDS             1649..2218
                     /gene="dcd"
                     /locus_tag="VIS73_11065"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY5763735.1"
                     /translation="MAIKSDKWIRRMAAEHGMIEPFSPQLVREANGQKIVSYGTSSYG
                     YDIRCADEFRVFTNINSTIVDPKAFDPNSFVEVSGKGYCVIPPNSFALARTVEYFRIP
                     RSVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGCAQVLF
                     FESDEVCETSYKDRGGKYQGQVGVTLPKI"
     gene            2296..4533
                     /locus_tag="VIS73_11070"
     CDS             2296..4533
                     /locus_tag="VIS73_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HEY5763736.1"
                     /translation="MRFHFPVIIIDEDFRSENASGLGIRALAKAIEDEGIEVLGVTSY
                     GDMSSFAQQQSRGSAFILSVDDEELVNEAEETIEELRAFVAEVRLRNEGIPIFLHGET
                     RTSRHIPNDVLRELHGFIHMFEDTPEFIARYVVREAKAYLESLPPPFFRALTHYAADG
                     SYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNAVEELGQLLDHTGPVAASE
                     RNAARITHADHLFFVTNGTSTSNKIVWHSTVAPGDIVVVDRNCHKSILHSIIMTGAIP
                     VFLMPTRNNFGIIGPIPKDEFRWKNIEKKIANHPFASKVKGKPRVLTITQSTYDGILY
                     NVEEIKEMLDGKIDTLHFDEAWLPHAAFHDFYGDYHAIGEGRPRCKESMVFSTQSTHK
                     LLAGLSQASQILVQESKERQLDRDVFNEAYLMHTSTSPQYAIIASCDVAAAMMEPPGG
                     TALVEESIREALDFRRAMRKVGGEYGNDWWFKVWGPNDLSEEGIEEREAWMLRAGEKW
                     HGFGKLAKNFNMLDPIKATIITPGLDVDGDFADDAGLPAAIVTKYLAEHGVIVEKTGL
                     YSFFIMFTIGITKGRWNTMLTELQQFKDDHDKNAPLWRVLPEFCQKHPRYERIGLRDL
                     CRQIHQVYKKNDVARLTTEMYLSNMEPAMRPADAFAKMAHREIERVPIDALEGRVTSV
                     LLTPYPPGIPLLIPGERFNRTIVRYLQFAREFNETFPGFETDIHGLVKTVNGDRVNYY
                     VDCVL"
     gene            complement(4512..>5715)
                     /locus_tag="VIS73_11075"
     CDS             complement(4512..>5715)
                     /locus_tag="VIS73_11075"
                     /inference="COORDINATES: protein motif:HMM:NF042682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HEY5763737.1"
                     /translation="MTGLAQKYGAVFSHIRLAASPEAIHAARIATGDADAVIGGDLVV
                     SAGPDALSRMVAGQTRAIINSAETPTAEFTRDPDWKLPGAAMRRDITDTIGAEAVSFV
                     DAHELAVALCGDALYMNALLLGTAWQQGMIPLALASIDRAFGINGVAVEKNREAFLWG
                     RRLAVDRDGVLRRAGMAPAAVAADGLDARIARREERLRAYQNAALAGRYRRLVDRVRF
                     TDTVGDGALSAAVAEQFFRLLAIKDEYEVARLLSDPDFLTGLRADFVASDGGAARLSF
                     HLSIPLLAGIDPESGRPRKRRFGQWLLPLLGVLARLRFLRGSALDPFGHSTERRAERA
                     LADEYERDISAALASLSAGNYETAVELARLPESIRGYGPLKMAAIEATRERRLELLAL
                     LAQSTQST"
BASE COUNT         1061 a         1798 c         1827 g         1029 t
ORIGIN      
        1 ggtctgaccg gccagcagcc cgagagcgcc gacggcaagt ctatggagcc gcttatggct
       61 cacggtattc aggcgatgtc gattggattc atgatcgacg tcgatacgcc gatggtctgg
      121 cgtggaccga tggtgacgca ggcgctcgag caactgctga ctcagaccaa ctggaacgac
      181 ctcgactatc tgatcgtcga catgccgccg ggaaccggcg acacccaact gacattggcg
      241 cagaaggtgc cggtgaccgg cgcggtgatc gtcaccacgc cgcaggacat tgcgctgatt
      301 gacgcgcgca agggcctgaa gatgttcgag aaggttggca ttccgattct cggaatcgtc
      361 gaaaacatgt cgatacacat ctgttcgaag tgcggccacg ccgagcacat cttcggcgaa
      421 ggcggcgcga cgcgcatgtg ccaggactac gacgtcgaac tgcttggcgc cctgccgctg
      481 gagatgtcga tccgggaaat ggccgatagc ggcaagccga cagtggtcgg tgcccccgac
      541 tcgaaggctg ccgaaatcta ccgggcgatc gcgcggctgg tcgcgatccg cgtcgccgag
      601 aaggcacgcg acatgagcag caagttcccc aatatcgtcg ttcagcacag ctaagtacta
      661 gtcggacgac actgtggact ggctcgccga ggtctggaat tttccactgc tgaagaccgg
      721 cgacggcgac ctgttactcg ggcgcctgct cggcctgctg ctgatcgtcg tcggcgcctg
      781 gtggggcgcg gtcgtcatcg agcgcatgat caagcgcgtc gcgcgcaatg cgcgcgctgg
      841 agccggaagt caggcggcga tcttcacctg ggcgcggatc gcgcgttatc tggtgtggat
      901 cgtcgcgacg atggtcggtc tgcagtacct tggcgtcgac ctggcgagct tcgcgctgat
      961 cggcggtgcg gtcggcgtcg gtatcggttt cggcctgcag aacatcttca gcaatttcat
     1021 ctcgggcgtc atcctgctgc tggagaagac gctcaaggtc ggcgacttcg tcgatctcga
     1081 gtcgggtgtt cgtggccatg tacgggagat cagccttcgt ttcacccgca tcagcaccaa
     1141 cgacaacgtc gatgtcgtcg ttcccaactc ggagttcgtc aacggccgcg tggtgaactg
     1201 gacccttgac gaccgcctgc ggcgtacgca tgttgccttt ggcgtggcct acggcaccga
     1261 caaggatgcc gtgcgcgagg cggccctgaa ggccgcacgt cgggtcgagt cgaccttcga
     1321 ggacgatacg cgcaaggccg aggtctggct ggtcggcatg ggcgagtcct cgctcgattt
     1381 cgagctggtg gtctgggtcg gtgccgattt cgttgcccgc ccggccagca tcgaggccaa
     1441 gtacctgtgg gcgatccacg acgagcttga gcaggccggg atttcgattc ccttcccgca
     1501 gcgcgacatc catttcgacc cgcagggcct gacggtcagg ctcgaccggg ggcgtggatc
     1561 gaattcagga acaggcggcg cgtgacaaga cccgccaagg gtgaaaaaat cgcggccttt
     1621 cttcagtcag caggcggcga caaagctcat ggccatcaaa tcggataaat ggattcgccg
     1681 catggcggcc gaacacggca tgatcgagcc gttttcgccc caactcgtgc gcgaggcaaa
     1741 cggccagaag atcgtttcct acggcacatc aagctacggt tacgatattc gctgcgccga
     1801 cgaatttcgg gtgtttacca acatcaactc gaccatcgtc gaccccaagg ccttcgatcc
     1861 gaattccttc gtcgaagtct cgggcaaggg ttattgcgtc attccgccga actcgttcgc
     1921 gctggcgcgc acggtcgagt atttccggat tccgcgctcg gtgctcaccg tctgcctcgg
     1981 caagagcacc tacgcgcgct gcggcatcat cgtcaacgtc acgcccttcg agccggaatg
     2041 ggaaggctac gtgacactcg agttctccaa tacaacgccg ctcccagcca agatctatgc
     2101 tggcgagggc tgtgcccagg tgctgttctt cgaatccgat gaggtctgcg agacgtcgta
     2161 caaggatcgt ggcggcaagt atcagggcca ggtcggcgtc acattgccga aaatctgacc
     2221 ggctgcggac cgaacggcgc tgtcaccatt tactgtgacc cgctcaggcg ggtcgcttgc
     2281 tcttgaggag ccgatatgcg tttccatttt ccggtcatca tcatcgacga ggattttcgt
     2341 tccgagaacg cgtctggcct cggcattcgg gccttggcca aggcgatcga ggacgagggc
     2401 atcgaggtgc tcggcgtcac cagctacggc gacatgagtt ccttcgcaca gcagcagagc
     2461 cgcgggtcgg ctttcatcct ttcggtcgac gacgaggaac tggtcaatga ggctgaggag
     2521 accattgaag aactacgtgc cttcgtcgcc gaagtccggt tgcgcaacga gggcatcccg
     2581 atcttcctgc atggcgagac gaggaccagc cgccacattc ccaacgacgt gctgcgcgag
     2641 ctgcatggct tcatccacat gttcgaagac acgccggagt tcatcgcccg ctacgtcgtg
     2701 cgcgaggcca aggcctacct cgagtcgttg ccgccgcctt ttttccgggc gctgacgcac
     2761 tacgcggccg acggttcgta ctcctggcac tgccccggcc actcgggcgg tgtcgccttc
     2821 ctgaaaagcc cggtcgggca gatgttccac cagttcttcg gcgagaacat gctgcgcgcc
     2881 gatgtctgca atgctgtcga ggaactcggc cagctgctcg accataccgg gcccgttgct
     2941 gcctcggagc gcaacgcggc gcgcatcaca cacgccgacc atctgttctt cgtcaccaac
     3001 ggcacctcga cctcgaacaa gatcgtctgg cattcgacgg tggcgccggg cgacatcgtc
     3061 gtcgtcgacc gtaactgcca caagtcgatt ctccactcga tcatcatgac cggcgcgatc
     3121 ccggtcttcc tgatgccgac gcgcaacaac ttcggcatca tcggcccgat cccgaaggat
     3181 gaattccgct ggaagaacat cgagaagaag atcgccaacc acccgttcgc ctcgaaggtg
     3241 aagggcaagc cgcgcgtgct gacgatcacc cagagcacct acgacggcat cctttacaac
     3301 gtcgaagaga tcaaggagat gctcgacggc aagatcgaca cgctgcactt cgacgaggcg
     3361 tggctgccgc acgcggcctt ccacgacttc tacggcgact accacgctat cggcgagggc
     3421 aggccgcgct gcaaggagtc gatggtcttt tcgactcagt cgacccacaa gctgctggcc
     3481 ggtctgtcgc aggcgtcaca gatcctggtg caggaatcca aggagcggca gctcgatcgt
     3541 gacgtgttca acgaggccta cctcatgcac acgtcgacca gcccgcagta cgcgatcatc
     3601 gcgtcctgcg acgtcgcggc ggcgatgatg gagccgccgg gcggcacggc gctggttgag
     3661 gaatcaatac gcgaggcgct cgacttccga cgggcgatgc gcaaggtcgg cggcgaatac
     3721 ggcaacgact ggtggttcaa ggtctggggc ccgaacgacc tgtccgaaga aggcatcgag
     3781 gagcgcgagg cgtggatgct gcgcgccggc gagaagtggc acggcttcgg caagttggcc
     3841 aagaacttca atatgctcga tccgatcaag gcaacgatta tcacgcccgg actcgacgtc
     3901 gacggcgatt tcgccgacga cgccgggctg cccgccgcga tcgttaccaa gtatctggcc
     3961 gagcacggag tcatcgtcga gaagaccggt ctttactcgt tcttcatcat gttcacgatc
     4021 ggcatcacca agggccgttg gaacacgatg ctgaccgaac tccagcagtt caaggacgac
     4081 cacgacaaga acgccccgct gtggcgcgtc ttgccggagt tctgccagaa gcacccgcgg
     4141 tatgagcgta tcggcctgcg cgatctgtgt cggcagattc atcaggttta caagaagaac
     4201 gacgttgcac gcctgaccac cgagatgtac ctgtcgaaca tggagccggc gatgcgcccg
     4261 gcagatgctt tcgccaagat ggcgcaccga gaaatcgagc gggtgccgat cgacgcgctc
     4321 gaaggccgcg tcaccagcgt gctgctgacg ccctatccgc cggggattcc gctgctgatc
     4381 ccgggcgagc gtttcaaccg gaccatcgtc cgctacctgc agttcgcgcg cgagttcaac
     4441 gagaccttcc cgggcttcga gacggacatc cacggtctgg tcaagaccgt caacggcgac
     4501 cgtgtcaact actacgtcga ctgcgtgctc tgagctaaca gtgccaacaa ctccaggcgg
     4561 cgttcgcggg tcgcctcgat tgctgccatc ttgagcgggc cgtagccgcg gattgattcg
     4621 ggcagccgag ccagctcgac cgccgtttcg tagttcccgg cggacaggct ggcaagcgcg
     4681 gcgctgatgt cgcgctcgta ttcgtctgcc aaggcccttt cggcgcggcg ttcggtgcta
     4741 tggccgaagg ggtcgagcgc actgccgcgc aggaagcgca ggcgcgcgag gacgccgagc
     4801 agcggcagca gccactgccc gaagcggcgc ttgcgcggcc ggccactctc tggatcgatg
     4861 ccggccagca gcgggatcga taggtggaac gagaggcgcg ccgcgccgcc gtcgctggcg
     4921 acgaagtcgg cccgcaggcc ggtcaggaaa tccgggtcgc tgagcagacg cgccacctcg
     4981 tattcgtcct tgatcgccag cagacggaag aactgctcgg cgaccgccgc cgacagcgcg
     5041 ccgtcgccga ccgtatcagt aaagcggacg cgatcgacca gacggcggta gcggcctgcc
     5101 agcgcggcgt tctggtaagc gcgcaagcgc tcttcccgtc gcgcgatgcg agcgtcgagg
     5161 ccgtctgcgg ccacagccgc cggcgccatc ccggcgcggc gcaggacgcc gtcgcgatcg
     5221 actgccagtc ggcggcccca gagaaaggcc tcacggtttt tctcgacggc cacgccgttg
     5281 atcccgaaag cgcggtcgat cgatgccagt gccaacggaa tcatgccctg ctgccaggcg
     5341 gtgccgagca gtaacgcgtt catgtagagc gcgtcgccgc acagcgcgac ggcgagttca
     5401 tgggcatcga cgaaggatac ggcttcagcc ccgatcgtgt cggtgatgtc gcgtcgcatt
     5461 gccgcaccgg gcagtttcca gtcggggtcg cgtgtgaatt cggcggtcgg ggtctcggcg
     5521 gaattgatga tcgcccgcgt ctggccggcg accatgcgcg acagcgcgtc ggggccggca
     5581 ctgaccacca ggtcgccgcc gatcaccgcg tcggcatcgc cggtggcgat ccgggcagcg
     5641 tggattgcct cggggctggc tgcaagccgg atgtgcgaaa acacggcgcc gtatttctgt
     5701 gcgaggccgg tcatg
//
LOCUS       DATDAA010000370         3676 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_481560,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000370 DATDAA010000000
VERSION     DATDAA010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481560"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VIS73_11080"
     CDS             complement(<1..725)
                     /locus_tag="VIS73_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY5763738.1"
                     /translation="MLLRIVAIVFPIFAVIAVGYVYGRLRKPDMTFANQLNMDVFIPA
                     LVFGALASKSFSIETYALLGFAGLLVVLGSGALAIPVARMLRVPVNTFVPPMMFNNSG
                     NMGLPLMMLAFGEAMLPAAVMLFLVENVLHYSLGTWMLDHRARLTTLWRIPVILAAIA
                     GLAVSLSGFQPWPPFLTAVRMLGDVSIPLLLFSLGVRLTDSAFQDLRIGLIGAVFCPL
                     SGVLIAYAAALLLGIEGIQRDML"
     gene            complement(785..2119)
                     /gene="hslU"
                     /locus_tag="VIS73_11085"
     CDS             complement(785..2119)
                     /gene="hslU"
                     /locus_tag="VIS73_11085"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEY5763739.1"
                     /translation="MSAMMPHEIVHELDKHIVGQDKAKRAVAIALRNRWRRAQVAEPL
                     RQEITPKNILMIGPTGVGKTEIARRLARLADAPFIKIEATKFTEVGYVGRDVDTIVRD
                     LVEVAIKDGRERAKRSVQSRAEDAAEERVLDALLPVARGAPFDGEAKPAEESATRQKF
                     RKKLREGDLDDKEIEIDVASPAMSAEIFSPPGMEELTGQIQSMFQNLGGGRKKSRKVK
                     VSEALRLLADEEASRLINDEEVKLDSLANVESNGIVFLDEIDKIAARSDIHGADVSRQ
                     GVQRDLLPLVEGTTVTTKYGMVRTDHILFIASGAFHLSKPSDLIPELQGRFPIRVELE
                     PLSVDDFERILVQTDACLTRQYEALLETEGAKLGFTAEGVRRLAEIAFAVNATTENIG
                     ARRLHTVMERLLEEVSFDAGRSGAMTVTVDRAFVDQRLAGLAANEDLSRYVL"
     gene            complement(2181..2717)
                     /gene="hslV"
                     /locus_tag="VIS73_11090"
     CDS             complement(2181..2717)
                     /gene="hslV"
                     /locus_tag="VIS73_11090"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEY5763740.1"
                     /translation="MESWHGTTILSVRRAGSVALGGDGQVTLGSIVIKGGARKVRRLH
                     QDRILAGFAGGTADAFTLFERFEAKLEKHQGNLLRSAVELAKDWRTDRMLRRLEAMLA
                     VADREHSLIITGLGDVLEPEHGIVAIGSGGPYAQAAARALIENTDFTPEEVVRRSLEI
                     AADLCIYTNRQFTIETLD"
     gene            complement(2740..>3676)
                     /locus_tag="VIS73_11095"
     CDS             complement(2740..>3676)
                     /locus_tag="VIS73_11095"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY5763741.1"
                     /translation="AIAFANDRDDETEATLRAAIADGEQAAAAERIWLMLFDFYLIAG
                     RRDAFAPLELEFAKRFEKQPPVWREISQKGGPKVAVAGSNAFSGDLVGANAEGLAQFE
                     QWLGADAKPKVDLSKIASVDVPGCERFLAAVQKARKQKKALEFSGCDSLAELLKPLVA
                     AGDEAQAYWRMLLECHQMQGQQEDFENLAVDFAVTFEISPPSWEAPPSTGKLAKPTPA
                     AAKGDDAFYLTGEIRGGRFDGLEAYLGEQAHPVVDMAAVTRMDFASAGTLLNLVAPHK
                     QRGVDLTLRHPNRLVAELLGLMGIGGVATLVFAKR"
BASE COUNT          695 a         1217 c         1115 g          649 t
ORIGIN      
        1 atcaacatgt cacgctgaat tccttcgatt cccagcagca gcgccgcggc gtaggcgatc
       61 aacacgccag agagcgggca gaagaccgcc ccgatcaggc cgatccgcag gtcctggaac
      121 gccgagtcgg tcaggcggac acctagcgag aacagcagca gcggaatcga gacatcaccg
      181 agcatgcgca cggcggtcag gaacggtggc cacggctgaa atcccgacag gctcaccgcc
      241 aacccggcga tcgcggcgag aattaccgga atccgccaga gcgtggtcag cctcgcccga
      301 tggtcgagca tccaggtgcc aagcgagtaa tgcagcacgt tctcaaccag gaaaagcatc
      361 actgccgccg gcagcatcgc ttcgccgaag gccagcatca tcagtggcag gcccatgttc
      421 cctgagttgt tgaacatcat cggcggaacg aaggtattga ccggtacccg cagcatccgc
      481 gccaccggta tcgccaatgc cccggagccc agcaccacca gcaggcctgc gaatcccagc
      541 agcgcgtagg tttcgatgct gaaggatttc gacgccagtg cgccaaagac cagcgccgga
      601 atgaacacat ccatgttcag ctggttggca aaggtcatgt ccggcttgcg caggcgccca
      661 tagacgtaac cgacggcaat gaccgcaaag atcgggaaga cgatcgcgac gattcgcagc
      721 agcactcact cgactccgat taattggacg gccaagccgt cggctccagg caggctggca
      781 gagatcagag gacgtagcgc gacaggtctt cgttcgcggc caagcccgcc aaacgctggt
      841 cgacgaaggc ccggtcaacg gtaacggtca tcgcgccgct gcgcccggcg tcgaaggata
      901 cttcctcgag cagccgctcc atcaccgtgt gcaggcgtcg cgcgccgata ttctcggtgg
      961 tcgcgttgac ggcaaaggca atctcggcaa gtcgacgcac gccctcggcg gtaaagccga
     1021 gcttcgcccc ttcggtttcc agcagcgctt cgtactggcg cgtcaggcag gcatcggtct
     1081 gcaccagtat ccgttcgaaa tcatcgaccg agagcggctc gagctcgacc cggatcggga
     1141 agcggccctg caactcgggg atcaggtctg agggcttgga caggtggaac gccccgctgg
     1201 caatgaacag gatgtggtcg gtccgcacca tgccgtactt ggtcgtgacg gtcgtccctt
     1261 cgacgagcgg cagcaggtcg cgctggacgc cctgccgcga cacatcggcg ccatggatgt
     1321 cgctgcgcgc ggcgatcttg tcgatctcgt caaggaaaac aataccgttc gattcgacgt
     1381 tggccagcga gtcgagctta acctcctcat cgttgatcaa ccgagacgcc tcctcgtcgg
     1441 cgagcagccg caaagcctcg ctgaccttga ccttgcgcga ttttttccgc ccgccaccga
     1501 ggttctgaaa catcgactga atctgcccgg tcagctcttc catgcccggt ggcgagaaga
     1561 tctcggcact catcgccgga ctggcgacat cgatctcgat ctccttgtcg tcgagatcgc
     1621 cctcgcgcaa cttcttgcga aacttctgcc gcgtcgcaga ctcctctgca ggcttggcct
     1681 caccgtcgaa tggcgcgccg cgtgcgaccg gcagcaaggc gtcgagaacc cgctcctcgg
     1741 ccgcgtcttc tgccctgctc tgcacacttc tcttggcgcg ctcgcggccg tccttgatcg
     1801 ccacctcgac gagatcgcgc acgatggtgt cgacatcgcg gccaacgtag ccgacctcgg
     1861 tgaacttggt cgcctcgatc ttgatgaacg gcgcatcggc gagccgtgcc agccgtctgg
     1921 caatctcggt cttgccaacg ccggtggggc cgatcatcaa gatgttcttc ggtgtgatct
     1981 cctgccgcag cggctcggcg acctgcgccc gccgccagcg attgcgcagc gcaatcgcca
     2041 ccgcacgctt ggccttgtcc tggccgacga tgtgcttgtc gagttcatga acgatttcat
     2101 ggggcatcat cgccgacatt cagggctccg ggttgtttcg gttgctttca acgaacgcca
     2161 ccagcaacgc aaggcggcga tcagtccagc gtctcgatcg tgaactgacg gttggtgtag
     2221 atgcagagat cggcggctat ctcgagcgag cgacggacaa cttcctcggg ggtgaaatcg
     2281 gtgttctcga taagtgcccg tgccgctgcc tgtgcgtagg ggccacccga gccgatggcg
     2341 acgatgccgt gctccggttc aagcacgtcg ccaagtccgg tgatgatcaa cgagtgctca
     2401 cggtcggcta ccgccaacat cgcctcaagg cgccgcagca tccggtcggt ccgccagtcc
     2461 ttggcgagtt cgacggcaga gcgcaacagg ttcccctgat gcttctcaag cttcgcctcg
     2521 aagcgctcga acagtgtgaa ggcatcggcc gttcctccgg cgaagccggc gagaatgcgg
     2581 tcctggtgca ggcgacgaac cttgcgcgcc ccacccttaa tgacgattga gcccagcgtg
     2641 acctgcccgt caccgcccag cgcgacgctg ccggctcgac ggacggaaag gatggtcgta
     2701 ccgtgccagg actccatcgc gacccgatcg actcgcgaat cagcgcttgg cgaagacgag
     2761 cgtcgcgacg ccgccgatgc ccatcaggcc gagcagttcg gcaaccaggc ggttgggatg
     2821 acgcagcgtc aggtccacac cgcgctgttt atgcggcgcg accagattga gcagggtgcc
     2881 ggcgctggcg aagtccatgc gcgtcaccgc cgccatatcg accaccggat gcgcctgctc
     2941 gccgagatag gcttccagcc catcgaaacg cccaccacga atttcgccgg tgagatagaa
     3001 cgcatcgtcg cccttggctg cggccggcgt cggtttcgcc agtttcccgg ttgacggcgg
     3061 cgcttcccag gacggcggcg agatttcgaa ggtgaccgcg aagtcgaccg cgagattctc
     3121 gaagtcctcc tgctggccct gcatctggtg gcactcgagc agcatgcgcc agtaggcctg
     3181 cgcctcgtcg cctgcagcaa ccagtggctt gagcagttcg gccagcgaat cgcaaccgga
     3241 gaactcaagc gccttcttct gtttccgcgc cttctggact gcagcgagga atcgctcgca
     3301 accgggcacg tcgacgctgg caatctttga caaatcgacc ttcggcttgg catcggcgcc
     3361 aagccactgc tcgaactgcg cgaggccttc cgcattggcg ccgaccagat cgccagaaaa
     3421 ggcgttgctg ccggcaacgg caactttggg gcctcccttc tggctaatct cgcgccagac
     3481 cggcggctgc ttctcgaaac gtttggcgaa ttcgagttcg agcggcgcaa acgcgtctcg
     3541 ccgcccggcg atcaggtaga aatcgaacaa catcagccag atacgctcgg ccgcggcggc
     3601 ctgctcacca tcggcgatcg ccgcgcgcag cgtcgcttcg gtctcgtcgt cgcggtcatt
     3661 agcgaaggca atcgca
//
LOCUS       DATDAA010000371         2622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_167140,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000371 DATDAA010000000
VERSION     DATDAA010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167140"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VIS73_11100"
     CDS             <1..358
                     /locus_tag="VIS73_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763742.1"
                     /translation="VNDRLRAKLDYWRIKPPSAYDFEDQFFPGQPGVSLESGGPSGDN
                     RYPVDTGRLWNHHSYENVLYGMDFLRDESREWFGDNRPPSAILKRVHGATRAAPGKLP
                     PHDVWLKRMVGMNDFR"
     gene            complement(430..999)
                     /locus_tag="VIS73_11105"
     CDS             complement(430..999)
                     /locus_tag="VIS73_11105"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEY5763743.1"
                     /translation="MNIDTPLRRLGAVDSEALREAILAQEDQAWKEDKYRQEEFEVHH
                     ATESIVVLFVDLERWPQVVVKQEPGWPRLADVALPLMNDIIRKHYPPGGTVIRAMAAK
                     LLAGGIINPHVDRHPSFHVGHRIHVPITTNPRVRFMIDGRPYQFEVGEAYEINNQKTH
                     SVMNKGAEDRITFIFDYVPPEQIEALNAA"
     gene            complement(1014..>2622)
                     /locus_tag="VIS73_11110"
     CDS             complement(1014..>2622)
                     /locus_tag="VIS73_11110"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5763744.1"
                     /translation="LISLDAETGKENWTRRVCDPTRSYSISDSPYVGGGKVFVGNAGS
                     ESPDGDRGYVAAYDANSGNELWRFYIVPSADPEENTSAAMKMAYATWSGDTLAKHGGG
                     GHSWNEMTYDPVSNLLFFGTSGSYPYVYKDRSPEGGDNLFLSSVVAVNADTGEYAWHY
                     QTVDQDSWDYNATMNIVLADLTIAGKSRETVLIAPKNGFHYALDRHTGELLTAGKFAK
                     VTWATHINMETGRPVYDPAGEYWKAEPGQAVAVWPNMWGAHNWNPMAYHPGLGLSYVP
                     VVDAPSVVEPRGDGTFKDDTVVFTEVDGKPFAPGKLVAFDPVSQSIRWTVEHELPYNG
                     GAMATAGNLVFQGNATGRFVAYAADTGETLWSVHTGSAINAAPASYLLDGTQYVVVPV
                     GGGGGVQFNYPEMHATEDAQGPFRLMAFSLGGDGALPGPTRADRALPEQPPLEASAEV
                     IASGKALYAENCKGCHGLNGVSRHGGTVPDLRYSSLEGHLAWNGVVIGGALRANGMPG
                     FGNLGVDEADAIRNYVLSLSERIRAGE"
BASE COUNT          415 a          835 c          887 g          485 t
ORIGIN      
        1 agtcaatgac aggctcaggg ccaagctcga ctactggcgc ataaagccgc cgtccgccta
       61 cgatttcgaa gaccagtttt tcccgggtca acccggtgtt tccctggagt cgggcggacc
      121 gagcggcgac aatcgctacc cggtcgatac cggccggctg tggaatcacc acagttacga
      181 gaacgtgctc tacggcatgg actttctgcg cgacgaaagc cgcgagtggt tcggtgacaa
      241 tcggccgccg tcggcgatac tgaagagggt acacggcgcg accagggccg cgcccggcaa
      301 actgccgccc cacgacgtgt ggctgaagcg catggtcggc atgaacgatt ttcgctgatc
      361 ccggtgacgc tgtccgcatg ccgccggcag catgcggagg tcatgcggcg cgtataaacg
      421 ggcctccact caggccgcgt tcagggcctc gatctgctcc ggcggcacgt agtcgaagat
      481 aaacgtgatc cggtcctcgg cgcccttgtt catcaccgag tgggtcttct ggttattgat
      541 ctcgtaggcc tcgccgacct cgaactggta ggggcggccg tcgatcatga aacgaacccg
      601 cggatttgtc gtgatcggca cgtggatgcg gtggccgacg tgaaacgacg gatgcctgtc
      661 gacgtgcggg ttgataatgc cgccggcgag aagttttgcc gccatcgcgc gaatcaccgt
      721 gccgcccggc gggtaatgtt tccggatgat gtcgttcatc agcggcagcg cgacgtcggc
      781 cagccgcggc cagcccggct cctgcttgac gaccacttgc ggccagcgtt cgaggtcgac
      841 gaacagcacg acgatcgact cggtcgcgtg atggacctcg aattcctcct ggcggtactt
      901 gtcttctttc caggcctggt cttcctgggc gaggatggct tcgcgcagcg cttcgctgtc
      961 gaccgcgccg aggcgcctca atggggtgtc gatattcatg ggaatcccgt tgcttactcg
     1021 ccggcccgaa tccgctcgga cagggacagg acatagtttc tgattgcgtc ggcctcgtca
     1081 acgccgaggt tgccgaaccc cggcatgccg tttgcgcgca gcgcgccgcc gatcacgacg
     1141 ccgttccagg cgaggtgccc ctcaagcgac gagtagcgca ggtccggcac cgtgccgccg
     1201 tgacgcgaga cgccgttcag gccgtggcag cccttgcagt tctccgcgta aagtgccttg
     1261 ccgctggcga tgacttcggc gctcgcctcg agcgggggct gctctggcaa ggcgcggtcg
     1321 gcgcgagtcg gcccggggag cgcgccgtcg ccgcccaggc tgaaggccat gagcctgaag
     1381 ggcccctggg catcctcggt ggcgtgcatt tcgggataat tgaactggac gccgccgccg
     1441 ccgccgacgg ggacgacgac gtactgggtg ccgtcgagca ggtagctcgc cggcgccgcg
     1501 ttgatggcgg agccggtgtg caccgaccag agcgtctcgc cggtatccgc cgcgtaggcg
     1561 acgaagcggc cggtcgcgtt gccctggaag acgagattgc cggcggtggc catggcgccg
     1621 ccgttgtatg gcagttcgtg ctcgacggtc cagcgaatgc tctgcgacac cgggtcgaag
     1681 gccaccagct tgcccggcgc gaagggcttg ccgtcgactt ccgtgaacac caccgtgtcg
     1741 tccttgaagg tgccgtcacc acgcggctcc accacggaag gcgcgtcgac caccggcacg
     1801 taagacaggc ccagcccggg atggtaggcc atcgggttcc agttgtgcgc gccccacatg
     1861 ttgggccaga cggcaacggc ctggccgggc tccgctttcc agtattcacc ggccggatcg
     1921 tacaccgggc ggcccgtctc catgttgatg tgcgtcgccc aggtgacctt cgcgaacttg
     1981 cccgcggtca gcagttcgcc ggtgtggcga tcgagcgcgt agtgaaagcc gttcttcggc
     2041 gcgatcagga cggtctcgcg cgacttgccg gcgatcgtga gatcggcgag cacgatgttc
     2101 atcgtggcgt tgtaatccca gctgtcctgg tcgaccgtct ggtagtgcca ggcgtattcg
     2161 ccggtatcgg cattgacggc aaccacggac gacaggaaga ggttgtcgcc gccctccggg
     2221 ctgcggtcct tgtagacgta gggataggag ccggaggtgc cgaagaacag caggttggag
     2281 acgggatcgt aggtcatctc gttccagctg tggccgccgc cgccgtgttt cgcgagcgtg
     2341 tcgcccgacc aggtcgcgta ggccatcttc atcgccgcgc tggtgttttc ttccgggtcg
     2401 gcgctcggca cgatgtaaaa gcgccacagc tcgttgccgc tgttcgcgtc gtaggccgcg
     2461 acgtaaccgc ggtcgccgtc cggcgattcg gagccggcgt tgccgacgaa taccttgccg
     2521 ccgccgacgt agggcgaatc ggagatcgaa tagctccgcg tcgggtcgca gacgcgcctg
     2581 gtccagttct ccttgccggt ttccgcgtcg agcgagatga gc
//
LOCUS       DATDAA010000372         3699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_237190,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000372 DATDAA010000000
VERSION     DATDAA010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237190"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..952
                     /locus_tag="VIS73_11115"
     CDS             275..952
                     /locus_tag="VIS73_11115"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY5763745.1"
                     /translation="MKYWPHLVLLGLIVALFLWERWKPRVVDRLEENALVLLMLLITG
                     VSFGQMVARYMFNTGWSAALEFTTVCFSWLILIGMSFGIKRGTHLGVDILIKALPNPA
                     TRWFGIFGAAMGVLYGMILLDATWLQLLGVETRSGAIDYWAKMYKINIGSEELRWPEF
                     IQEWFGVKDRVQRWLVLIILPISLALIAFRSLQAMIEIYRGDRTTLIAGHEAEDLVKE
                     NLGVLKD"
     gene            962..2581
                     /locus_tag="VIS73_11120"
     CDS             962..2581
                     /locus_tag="VIS73_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY5763746.1"
                     /translation="MEPIFLFGMVLTLLFLGVPVGLSLGLSAILYITFFTTDSLSSVA
                     ISLFGVGQHYTLLAIPFFILASSFMSTGGVAKRLIRFAIATVGHLRGGLAMASVLACM
                     MFAALSGSSPATVVAIGTIAIAGMRKVGYSKDFAAGLIANAGTLGILIPPSIVMVVYA
                     ASVDVSVGRMFLAGVIPGIVAGLMLMVTIYIVARWKNLPAEPWPGISEFLASGKDAFW
                     GLFLIIIIMGGIYGGVFTPTEAAAAAAVYAAFVAVFIYRDMGPFKDKPWVREGDSKES
                     AVGFKASVYGLTFFVVFSILSFFAVGDNKAVSSATRYLWGAGLSVGFVIGYLFWRGHS
                     GSPIRAAHLLAVSTPIWAYNLKLMAKGLGKALTGDDTRHTVLEASKTTVMLMFIIANA
                     LLFAHTLTAERIPQTITEWMLGAGFTWFTFLIAVNILLLIGGQFMEPSGLLLIVAPVV
                     FPIAIELGVDPIHLGIIMVVNMEIGMITPPIGLNLFVTSGITGMSLGRVVKAALPFVG
                     VLFIFLILVTYVPALSTWLPYLLMGPEVITK"
     gene            2836..>3699
                     /locus_tag="VIS73_11125"
     CDS             2836..>3699
                     /locus_tag="VIS73_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5763747.1"
                     /translation="MPLSHLTETLAASLASLTRGASTPSPFVVMVIDDQATAREILAA
                     IVRSIDPQAQVHKFEHPVDAVSWAASNDVDLVLTDLRMPVMDGVEVIRRLRVLPHCDD
                     VPMVVVTMVEERAIRHAALDAGATDFLNKPLDKHEAIVRCRNLMTMRGQQKLLRDRAQ
                     HLQMRVDQATVQLRNSELQTLFMLAKAGEFRDSDTGNHVIRVSQISGLIARAIKADEA
                     DIIETSALLHDIGKIGISDTILLKPGRLTAEEMDVMREHTRIGYSILLEGKTHYTEIG
                     AEIAISHHERFD"
BASE COUNT          653 a         1217 c         1106 g          723 t
ORIGIN      
        1 cggcacagcg caaggcctgg gtcgacgcga tgaagccagt gtggcagcag ttcgaaggcg
       61 agatcggcaa ggacatcgtc gaagccgctg ccggctcctg agcctctacg gcgggcggcg
      121 tgggttcccg cgtcgcccga tcttccgctc gctgtccgga cctggtttcc ggccggcggg
      181 cggtgccgat aaccggcgcg cagtgctggc gcgctgacaa tcggccccgc agtgaagcta
      241 tcgtccgagc aaccgccaac ctagggagca agccatgaag tactggcccc accttgttct
      301 cctcggcctc atcgtcgccc tcttcctgtg ggagcgatgg aagccgagag tcgtagaccg
      361 tctcgaagaa aacgccctgg tgctgctgat gctgctgatc accggcgtct cattcggcca
      421 gatggttgcc cgctacatgt ttaacacggg ctggtccgca gcactcgaat tcaccaccgt
      481 ctgtttcagc tggctgatcc tcatcggcat gagcttcggc atcaagcgcg gcacccacct
      541 gggcgtcgac atcctgatca aggccttgcc caacccggca actcgctggt tcggcatctt
      601 cggcgcggcg atgggtgtgc tctacggcat gatcctgctc gacgccacct ggctgcagct
      661 gctcggcgtc gagacgcgct ccggcgccat cgactactgg gcgaagatgt acaagatcaa
      721 catcggcagc gaagagttgc gctggccaga attcatccag gaatggttcg gcgtcaagga
      781 tcgggtccag cgctggctgg tgctaataat cctgccgatc agcctggcgc tgatcgcctt
      841 ccgcagcttg caggcgatga tcgaaatcta ccgcggcgat cgcaccacgt tgatcgccgg
      901 ccatgaagcc gaggacctcg tcaaagagaa cctcggcgta ctcaaggact gagacgacga
      961 catggaaccc atcttcctct tcggcatggt tctcacgctg ctcttcctcg gcgtcccggt
     1021 gggcctttcg ctcggcctgt ccgcgattct ctacattacc ttcttcacca ccgattcgct
     1081 gtcctcggtg gcgatctcgc tgttcggcgt cggccagcac tacacgctgc tagcgattcc
     1141 cttcttcatc ctagcctcgt ccttcatgtc caccggcggt gtcgccaagc ggctgatccg
     1201 ctttgccatt gccacggtcg ggcacctgcg cggcggcctg gcgatggcct cggtgctggc
     1261 gtgcatgatg ttcgccgcgc tctcgggctc gtcaccggca acggtggtgg cgatcggcac
     1321 aatcgcgatt gccggaatgc gcaaagtggg ctatagcaag gatttcgccg ccggcctaat
     1381 cgccaacgcc ggaacgctgg gcatactgat tccgccatcg atcgtcatgg tcgtctacgc
     1441 ggcctcggtc gacgtttcgg tcggccggat gttcctcgcc ggcgtcattc ccggcatcgt
     1501 cgccggattg atgctgatgg tgacgatcta catcgtcgcc cgctggaaga acctgccggc
     1561 cgaaccctgg cccggcatca gcgagtttct ggcgtcgggc aaggatgcgt tctggggcct
     1621 gttcctgatc atcatcatca tgggcggcat ctacggcggc gtcttcacgc cgaccgaagc
     1681 agcagcggcg gcggccgtct acgcggcctt cgttgcggtc ttcatctacc gcgacatggg
     1741 tccgttcaag gacaagccct gggtgcgcga gggggacagc aaggaaagcg ccgtgggctt
     1801 caaggcctcg gtctacggac tcacattctt cgttgtcttc tcgatcctct ccttcttcgc
     1861 cgtcggcgac aacaaggcag tcagttcggc gacgcgctac ctgtggggcg ccggactgtc
     1921 ggttggcttc gtcatcggct atctgttctg gcgtggccac agcggttcgc cgatccgcgc
     1981 cgcgcatctg ctcgcggtca gcacaccgat ttgggcctac aacctcaagc tgatggccaa
     2041 gggactgggc aaggcgctaa ccggcgacga cacccggcat acggttctcg aagcctcgaa
     2101 gaccacggtg atgctgatgt tcatcatcgc caacgccttg ctgttcgccc acacgctgac
     2161 tgccgagcgg attccgcaga cgatcaccga gtggatgctc ggcgccggct tcacctggtt
     2221 caccttcctg attgccgtga acatcctgct gttgatcggc ggccagttca tggagccctc
     2281 aggcctgctg ctgatcgtcg ccccggtggt gttcccgatc gccatcgaac tcggcgtcga
     2341 tccgatccac ctcggcatca tcatggtggt aaacatggag atcggcatga tcacgccacc
     2401 tataggcctg aacctgttcg tcacctcggg gataacgggg atgagtctgg gcagggtggt
     2461 caaggcggcg ctgcccttcg ttggcgtgct cttcatcttc ctgatcctcg tcacctacgt
     2521 gccggcgctg tcgacctggc tgccgtacct gctgatgggc ccggaagtca ttaccaagta
     2581 gcgccacgga cagacgccct cagggcatct cgccccgcaa cgcccggcca gacagccggg
     2641 cgtttttttc gcggcacagg atttccggct gcacaccccg ccgaatagag ccgggacggc
     2701 tcattcaggg gcgaaaggca agcgttgcac gagtaaacgc gacgtaattc ctgtgcgcca
     2761 acccgtcgcc ggactacaaa taggcgacta gaatcggcgg gaagccaaca ataacttccg
     2821 aatggccctg atttcatgcc tctttcacac ctgaccgaaa cgctggcggc gagcctggca
     2881 tccctgacgc ggggcgcttc gacgccatcg cccttcgtcg tgatggtcat cgacgatcag
     2941 gcgacggcac gcgagatcct cgccgccatc gtccgcagca ttgatccgca agcgcaggtt
     3001 cacaagttcg agcatccggt cgatgccgtc tcctgggccg ccagcaacga tgtggatctg
     3061 gtgcttaccg acctgcgcat gccggtcatg gacggcgtcg aggtgatccg ccgcctgcgc
     3121 gtgctgccgc actgcgacga cgtgccgatg gtcgtggtga cgatggtcga ggagcgcgcc
     3181 atccgccacg ccgcgctcga cgccggtgcc accgacttcc tcaacaaacc gctcgacaag
     3241 cacgaggcga tcgtccgttg ccgcaacctg atgaccatgc gcggccagca gaagctcttg
     3301 cgcgaccgcg cccagcattt gcagatgcgc gtcgatcagg caacggtcca gttgcgcaac
     3361 agcgaactgc agacgctgtt catgctggcc aaggccggcg aattccgcga cagcgatacc
     3421 ggcaaccacg ttatccgcgt atcgcagatc tccggactga tcgcgcgtgc aatcaaggcc
     3481 gacgaggccg atatcatcga gacttcggcg ctgctccacg acatcggcaa gatcggcatc
     3541 tccgatacga tcctgctcaa gcccggccgc ctgacggccg aggaaatgga cgtgatgcgc
     3601 gagcacacac gcatcggcta ctcgatcctg ctggaaggca agacgcatta caccgagatc
     3661 ggcgccgaaa tcgccatcag tcaccacgaa cgattcgat
//
LOCUS       DATDAA010000373         3883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_97156,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000373 DATDAA010000000
VERSION     DATDAA010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97156"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VIS73_11130"
     CDS             <1..434
                     /locus_tag="VIS73_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763748.1"
                     /translation="LDIRFKRINYNESVRPKIYDRMISERRQIAERFLSEGNGEAARI
                     RGNRLRDLNKIQSEAYRQVEEIRGVADAKATEIYAGAYNQSPEAVTFYEFTRTMQAYQ
                     AIIAENTTLVLSTDSDLFKFLKGMSPDGAGVPLVSTDAIRQ"
     gene            499..>3883
                     /locus_tag="VIS73_11135"
     CDS             499..>3883
                     /locus_tag="VIS73_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HEY5763749.1"
                     /translation="MSKWIRDLDDAHRQAGAANRARMISVVDYFARHRANSPPWQDTA
                     PVREEIFGVERLDRHAESLAAAQPVTTKPQHVTSLHARLYGNAKALLRAYRVCAAELE
                     RGRDVVPAAEWLLDNYHLVEAQIREIRNDLPPGYYRQLPKLAEGPFAGYPRVFGLAWA
                     FVAHTDSHFDPVILRRFIVAYQRVQPLTIGELWAVAITLRIVLVENLRRLADQIIAGQ
                     AARADADALANRLLVSGDARSALEADILSRSSASLSTTFAAQLAKRLRDQDPKETPAL
                     GWLEERLGLQGQSIDGAVQHEQRSRGASNVTVRNIITSMRLISDIDWAEWFESVSLVD
                     QRLCANSAFAAMDFPTRNAYRSAIEELARGSSSTELEVAEQALTAARLAAAGAVEATE
                     AERVADPGYHLIAKGRRALERTIGFRPPARLWVTRVGRRLGIGGYVGTILLTTVTLLL
                     LAMWALSLAGLGLGWLTLFALVAFLPATEAATAFVNRAITWGFGAITLPGLDLTEGVP
                     QSLRTLVAVPTLLTNEADILEQVERLEVHHLAGAGGDLTFALLSDWIDADQEVLPRDA
                     HLLAIATEAIGQLNRRYGPGPAGDRFLLLHRRRVFNASESQWMGWERKRGKLHELNRL
                     LLGATDTTFVSVAGCPFQVPTGVRYVIALDADTRLPRDVAAKLIGKMAHPLNRPMFCK
                     VERRVVDGYAILQPRVTPSLPVGREGSLYQRVFSSPSGIDPYAAAVSDVYQDLFGEGS
                     YTGKGIYDVDAFEAALSGRVPENALLSHDLFEGIFARAGLASDVEVVEEFPSRYDVAG
                     KRQHRWTRGDWQLLPWIFGRSTANGSFPSIGRWKMLDNLRRSLLAPFTLMAFGLCMLL
                     PAPAGVVGALLVLVAMAIPPFLSTFFAVLPHRSGIRLDRHMRMLGADLYLAASQTLLA
                     VVFLADNAWRMGDAVARTLVRLSLTRRHLLEWTTAAQATAGPRLDYRGFYRQMAGGTS
                     LGLGMTAGVIGFAPHAWPLAAPFGLLWLAAPALALWASRAPTVGRRLAVSDTDARYLR
                     LTARRTWRYFETFVTAAENNLPPDNFQEDPTPLVARRTSPTNLGLYLLSAVAARDFGW
                     AGTMKTVARLEATLESMQSLPRYRGHFFNWYGTRDLKVLE"
BASE COUNT          664 a         1260 c         1242 g          717 t
ORIGIN      
        1 tgctcgatat acgattcaaa cggatcaact acaatgaaag tgtgcgcccg aagatttacg
       61 accggatgat cagcgaacgg cgtcagatcg ccgagcgctt cctgtccgag ggcaacggcg
      121 aggccgcccg catccgcggc aaccggctgc gcgatctgaa caagatccag tcggaggcct
      181 accggcaggt cgaagagatc cgcggcgtgg cggacgcgaa ggccaccgag atctacgcgg
      241 gcgcttataa ccagagtccc gaagcggtga cgttctatga gttcacgcgg accatgcagg
      301 cctaccaggc gatcatcgcg gaaaacacca cgctggtgct ttccacggac agcgatctgt
      361 tcaagttcct caagggcatg agcccggatg gcgcgggggt cccactggta agcacagacg
      421 cgatacggca ataacacgtg agcgacgtgc gccgcacctt cgttccgcgt tcggcgaagg
      481 agcagaacct acccgccaat gagcaaatgg attcgcgacc ttgacgatgc gcatcgtcaa
      541 gcaggcgcgg ccaatagagc gcgcatgata tcggtcgtgg actactttgc ccggcaccgc
      601 gcgaattcac caccatggca agacaccgcc ccggttcggg aggagatctt cggggtcgaa
      661 cggctcgacc ggcacgccga aagcctcgcg gccgcgcagc cggtaacgac taagccgcaa
      721 cacgtcactt ccttgcatgc gcgactgtat ggcaatgcca aggctttgct cagggcgtat
      781 cgcgtctgtg ccgcagagct cgagcgcggc cgcgatgtcg tgccggcagc ggaatggctc
      841 ctcgacaact atcatctggt cgaggcgcag atccgcgaga tccgaaatga tctgcccccc
      901 ggctactatc gccagctgcc gaagctggcc gaggggcctt tcgccggtta tccgcgggtg
      961 tttggccttg cctgggcgtt tgtcgcgcac accgacagcc acttcgatcc cgtcatactg
     1021 cgacggttca ttgttgccta tcagcgggtt cagccgctca cgatcggcga gctgtgggcg
     1081 gtggcgatca cgttgcggat cgttctcgtg gagaatcttc gccggctggc cgaccagata
     1141 atcgcgggac aagcggcgcg cgctgacgcc gatgcgctgg ccaacaggct cctggtatca
     1201 ggggacgcgc ggtcagcgct cgaagccgac atcttgtcgc gctcgtccgc gtccttgtcc
     1261 acaaccttcg ccgcgcaact cgcaaagcgg ctgagagacc aggatccgaa agagacgcct
     1321 gcgcttggat ggctggaaga gcgcctcgga ctccaagggc aatccatcga cggggcggtg
     1381 cagcacgagc aacgcagccg cggggcatcc aacgtcaccg tgcgcaatat catcaccagc
     1441 atgcggctga tctccgatat cgactgggcc gagtggttcg agagcgttag cctcgtcgac
     1501 cagcgcctgt gcgcaaatag cgcctttgcc gcaatggatt ttcctacccg caacgcctac
     1561 cgaagcgcga tcgaggagct ggcccgcggg tcgtcgtcca cagagctcga ggtcgccgag
     1621 caggcgctga ccgcggcgcg tttggcggcc gccggggcag tcgaagccac ggaggcggaa
     1681 cgtgtcgccg atcccggcta tcacctgatc gccaagggca ggcgcgcgct ggagcggaca
     1741 atcgggtttc ggccgcctgc tcgactgtgg gtcacccgcg ttggccgccg tcttggtatc
     1801 ggtggctatg tggggacgat cctgcttacc acggtaacct tgctgcttct cgcgatgtgg
     1861 gcgctttcat tggccggcct aggtctcggc tggctgacgc tatttgcgct ggttgccttc
     1921 ctcccggcta cggaggcggc gactgccttt gtgaaccgcg cgatcacctg gggttttggc
     1981 gcaataaccc tgccgggtct ggatctgacg gaaggcgtgc ctcaatcgct tcgaacgctc
     2041 gttgccgtgc cgacgttgct gacgaatgag gcggatattc tggagcaggt cgagcggctc
     2101 gaggttcacc acctggccgg cgccggcggc gatctcacct tcgcccttct gtcggactgg
     2161 atcgatgccg accaggaagt cctcccgcgt gatgcccatc ttctcgccat cgccaccgag
     2221 gcgatcgggc agctgaaccg ccggtacgga ccgggtcccg ctggcgatcg ctttttgctg
     2281 ttgcatcggc ggcgcgtgtt caatgcgtcc gagagccaat ggatggggtg ggagcgcaag
     2341 cgaggcaagc tgcacgagct gaaccgacta ctactcgggg ccaccgacac gacattcgtg
     2401 tctgtcgccg ggtgcccgtt ccaggtacca accggcgtgc gctatgtcat cgcgctcgac
     2461 gcagacacca gactaccgag agatgtagca gcgaagctga ttggcaagat ggctcatccg
     2521 ctgaatcgcc cgatgttttg caaagtcgag cgacgcgtcg ttgacggtta cgccatcctc
     2581 caaccccggg taacgccctc gctgccggtc ggccgcgagg gttcgcttta tcaacgagtc
     2641 ttttccagcc ccagcggtat cgatccttac gcagccgccg tctccgacgt ctatcaagac
     2701 ctcttcggtg aggggtcgta taccggcaag gggatctacg acgtcgatgc cttcgaggca
     2761 gcgctctcgg gccgggtgcc ggagaatgcg ctgctcagcc atgacctgtt tgaagggatc
     2821 ttcgcccgcg cgggtcttgc ctcggatgtc gaggtcgtcg aggaattccc ctctcgctac
     2881 gacgtcgccg gcaaacgcca gcatcgctgg acacgcggcg actggcagct cctgccctgg
     2941 atattcggac gctcgaccgc aaacggatcg tttccgtcga tcggccgctg gaaaatgctg
     3001 gacaatctgc ggcgatcgct gctggcgcca ttcacgttga tggccttcgg cttgtgcatg
     3061 ctgctgccgg cgccggccgg tgtcgtgggc gcccttctcg tcctcgtcgc catggcgatc
     3121 ccgccctttc tatcgacctt cttcgccgta ctgccgcatc gctcggggat ccgcctggac
     3181 cgccacatgc gcatgctggg cgcagaccta tatctggcgg cttcgcagac actgcttgcg
     3241 gttgtattcc tggccgacaa cgcctggcgt atgggcgacg ccgtcgcgag aacgctggtc
     3301 cggctgtcgt tgacgcgccg ccatctgctc gagtggacga ccgccgcgca agcgaccgcc
     3361 ggtcctcggc tcgattaccg gggcttctac cgacagatgg cgggcggcac ctcgctgggc
     3421 cttggcatga cagccggagt aatcggattc gcgccgcacg cttggccact cgccgcgccc
     3481 ttcggcctgt tgtggctggc cgcgccagcg ctcgccttgt gggccagccg cgcgccgacc
     3541 gtcgggcggc ggctcgccgt ttcggacacg gatgcacgct acctgcgcct gacggcgcga
     3601 cggacctggc gctacttcga gaccttcgtg accgcggcgg agaacaatct gccgccggac
     3661 aatttccagg aggacccgac gccgctcgtc gcccgacgca cctcgccgac caacctgggc
     3721 ctgtacctgc tttcggcagt ggccgcgcgc gatttcggct gggccgggac gatgaaaacg
     3781 gtggcgcgac tggaagcgac gctcgagtcc atgcaaagcc tcccgcggta ccgcgggcat
     3841 ttcttcaact ggtacgggac acgggacctg aaggtgctcg agc
//
LOCUS       DATDAA010000374         2495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_342083,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000374 DATDAA010000000
VERSION     DATDAA010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342083"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VIS73_11140"
     CDS             <1..680
                     /locus_tag="VIS73_11140"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY5763750.1"
                     /translation="AAIIIMAVIGLINFQGIHHAWLASRHDGIAAIVTFVATLGFAPH
                     LDNGILIGAGLAIGLFLYRTMQPRVAILGRHADGNLRDREVFPELPKCPEVIILRFDG
                     RLYFANVPYFEDAVLAAVADNPQAQILLIAGSAINEVDASGQEALRNLVRRLRGAGVE
                     VMFSGLKKQIYDVLRATHLEEDLGLTNFYATDEEALAAIGARLGAETIAPLLCRPVAG
                     VQAQSGQ"
     gene            677..2134
                     /locus_tag="VIS73_11145"
     CDS             677..2134
                     /locus_tag="VIS73_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY5763751.1"
                     /translation="MTERVSLSRAAQLLGTTRAALQGKIREGLLPAFDGEVSLADLRR
                     LYPDLEVEASGAYEKVADIKKHAFGRRILERALPSQEALALRIFDQSEELAGVRRHLE
                     QYHALIAATIAALETEPAGALRDATLARLRGGLAEVLAHEHDDSLETMQNILRVVSAQ
                     VTVRPSGRQFLVEGNDSLLQAGLKAGLRLPYGCGSGSCGLCKARVVGGTVRRIAHSDY
                     GLSEAERQSGVVLTCVCAPNADLTLEVLEANDAGDIPHQEITATVRKVETLAPDTRLL
                     HLQTPRSNRLRFLAGQSLTLGLTVAGEDIAATLPIASCPCDDRNLQFHVGRDDSDFSR
                     LLFADRVRPGETLNLRGPLGEFVISGEGIRPYVFVAADLGFAPIKSLIEHAMAIDDND
                     RFALVWASSRSDGAYLDKLCRMWAQAFDDFHYLPAASPSIADAASAAAAATREIPFLG
                     DAEIFVAGPEAFVAAVESALQLSGIACDRLHGFGF"
     gene            2201..>2495
                     /locus_tag="VIS73_11150"
     CDS             2201..>2495
                     /locus_tag="VIS73_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763752.1"
                     /translation="MAEIGIHGIAVLLLTVGAFVSFAMQRVPIETTALVVIAALALGF
                     ALFPYETAGGALKPTAFFLGFGHEALVAICGLMILGQGLTVTGALEPVARRLSV"
BASE COUNT          395 a          913 c          775 g          412 t
ORIGIN      
        1 tggcagcgat catcatcatg gcggtgatcg gcctgatcaa ctttcagggc atccatcacg
       61 cctggctcgc cagccgccac gacggcatcg ccgcgatcgt caccttcgtc gccacgctcg
      121 gtttcgcgcc acacctcgac aacggcatcc tcatcggcgc cgggctagcg atcggccttt
      181 tcctctaccg gacaatgcag ccgcgcgtcg cgatccttgg ccgccacgcc gacggcaacc
      241 tgcgcgaccg cgaagtcttt ccagagctgc cgaaatgccc ggaggtgatc atcctccgtt
      301 tcgatggccg cctctacttc gccaacgtcc cctattttga agatgccgtg cttgccgccg
      361 tcgcggacaa tccgcaggcg cagatcctgc tgatcgccgg cagcgccatt aacgaggtcg
      421 acgcatcggg gcaggaagcg ctgcgcaacc tggtccgccg cctgcgcggc gccggcgtcg
      481 aggtcatgtt cagtggcctg aagaaacaga tctacgacgt attgcgcgcg acgcacctcg
      541 aggaagacct tgggctgacc aacttctacg ccactgacga agaggcgctg gcggccatcg
      601 gcgcgcgcct cggcgccgaa accattgcgc cgctgctgtg ccggccggtc gccggcgttc
      661 aggcgcagag cggtcaatga ccgagcgcgt ctcgctctcc cgcgcggcgc aactgctcgg
      721 caccactcgc gccgcactgc agggaaagat ccgcgagggg ttactgccgg ccttcgacgg
      781 cgaggtgtcg ctcgccgact tgcgccgcct ctacccagac ctcgaggtcg aggcgtccgg
      841 ggcctacgag aaggtcgccg atatcaagaa gcacgccttc ggccgtcgca tcctcgagcg
      901 tgcgctgccc tcccaggaag cgctggccct acggattttc gaccagagcg aggaacttgc
      961 cggggtgcgc cggcatctgg agcaatatca cgcgctgatt gcagcgacga ttgccgccct
     1021 cgaaacggag cctgccggcg ccctccgcga cgcgacactg gcacgactgc gcggcggcct
     1081 cgccgaggtc ctcgcccacg aacacgacga cagcctggag acgatgcaga acatccttcg
     1141 cgtagtgtcg gcacaggtaa cggtgcgccc cagcggtcgc cagttcctcg tcgaaggcaa
     1201 cgacagcctg ctgcaggccg gtctcaaggc tgggctgcgt ctgccctacg gttgcgggtc
     1261 gggaagctgc ggcctgtgca aggcgcgcgt tgtcggcggc acggtgcggc ggatcgcgca
     1321 ttcggactac ggactctccg aagccgagcg gcaaagcggc gtggtcctca cctgcgtctg
     1381 cgcgccgaac gccgacctga cgctggaagt actcgaagcc aacgacgccg gcgacattcc
     1441 acaccaggaa atcaccgcca ccgtgcgcaa ggtcgagacg ctcgcgccgg acacgcgcct
     1501 cctgcacctg cagacgccgc gcagcaaccg gctgcgtttc ctcgccggac aatcgctgac
     1561 gctgggcctg accgtcgccg gcgaggacat cgcggcaacg ctgccgattg ccagttgccc
     1621 gtgcgacgac cgcaacctgc agtttcacgt cgggcgcgac gacagcgact tctcgcgcct
     1681 gctcttcgcc gaccgggttc ggccgggcga aacgctcaat ctgcgcggcc cgctcggtga
     1741 gttcgtcatc agcggcgaag gcatccgccc ctatgtcttc gtcgccgccg atctcggctt
     1801 cgcccctatc aagagcctca tcgagcacgc catggcgatc gacgacaacg accgcttcgc
     1861 gctggtctgg gccagcagcc gcagcgacgg cgcctatctc gacaagctct gccgcatgtg
     1921 ggcgcaggcc ttcgacgact tccattacct cccggcggcc tcgccatcaa ttgccgacgc
     1981 ggcatcggct gccgccgctg cgacgcgcga aatccccttc ctcggcgatg ccgaaatctt
     2041 cgtcgccggc cccgaggcat tcgtcgcggc ggtcgaatcg gcgctccagc tcagcggcat
     2101 cgcctgcgac aggctgcacg gcttcggctt ttaggcgaga atcgcccggc ggccccggcg
     2161 gccgccacgt cctcctgacg cagaaggcga ctcccccacg atggctgaaa tcggaatcca
     2221 cggcatcgcc gtcctgctgc tgaccgtcgg cgccttcgtc tcctttgcga tgcagcgggt
     2281 gccgatcgag accacggcac tggtcgtcat cgccgcgctg gcgctcggct tcgcgctgtt
     2341 tccctacgag acggccggcg gcgcgcttaa gcccacagcc ttcttcctcg gctttggcca
     2401 cgaggcgctg gtcgccatct gcggcctgat gatcctcggc cagggtctca ccgtcaccgg
     2461 cgccctcgaa ccggtcgccc ggcgcctctc ggtgc
//
LOCUS       DATDAA010000375        15256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_97170,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000375 DATDAA010000000
VERSION     DATDAA010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 15256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15256
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97170"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VIS73_11155"
     CDS             complement(<1..1157)
                     /locus_tag="VIS73_11155"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5763753.1"
                     /translation="MAIEDTTESREQSPKTWELLEKLATAGDSKTLEAYVDSIGPSEA
                     FRALLRLAPEHREKVLTTLSPEEAADLIEEIPDEHASDLIESLPAKQAASILSEMDVD
                     DQVDVIADMHDDDAEAILAEMAPEDAAHVRRLIRYDPDVAGGLMSTDYLAYGESALVG
                     DVIDDLSKRADEREEPEHDAHVYVVSTTGKLIGVLDLRDLVLARRTTPLASILKSSLF
                     VRAGATLDELETFFDRYDFQAVPVIDAHQELVGVVGRDEVAEAIAIRSQEEYAKSKGI
                     IGGDEIRSLPVLTRAGRRLSWLSLNIVLNIVAASVIAMYFDTLSAAIALAVFLPIVSD
                     MSGCSGNQAVAVSMRELSLGIVKPYEVLRVWIQEIKVGVLNGITLGILLGAV"
     gene            complement(1365..2345)
                     /locus_tag="VIS73_11160"
     CDS             complement(1365..2345)
                     /locus_tag="VIS73_11160"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY5763754.1"
                     /translation="MNFMKKIVPAAIALAFAGAAQAQVSIYGLIDMSVGKNEVVDGKT
                     AKTDIHSGGDDSQSQGNSTTRIGVKGSFDVDKDVKAHFKFETNGITSNGAVNGPAFFN
                     RQAWLGFSHTKMGEIRVGRQDSIPFQVMGAFDFNGQSNGATSAYSGVGVWATDRQSRS
                     VQYISPEMSGFKAQVGYVPRGDNTDLRDASTYDDNTRGVASAAVTYSGIKNLVIGASH
                     EGKKTLNGEDFNSVAASYDFGFLKAMAGYTDAGLNATGPTLGVTAPVAGFTMGAHVAQ
                     NKDTKAKALELFINREIFKNTYAYAEALRLDNKAPAVDLNSYAVGVIFVF"
     gene            2697..3488
                     /locus_tag="VIS73_11165"
     CDS             2697..3488
                     /locus_tag="VIS73_11165"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5763755.1"
                     /translation="MKKIVLAGLVLAFATGFAQAKEWKQIRLATEGAYPPFNETAPDG
                     SVRGFDIDIGNAVCAEMKAKCSWVKQEWDGMIPALLSRKFDAIIASMSITEERKKKVD
                     FSDKYYTTPLALVAKEGSPLVPTAESLKGKKVGVQRGTIADNFATKFWEPKGVSVVRY
                     AKQEEAYLDLGAGRMDAGFSDMLEAEGGFISKPEGKGFAFVGNKVFGKTAEEKAVIGE
                     GIGIAVRKKDAELKAAFNKAIAAIRANGTYNTIRAKYFKYDIYGE"
     gene            3607..4299
                     /locus_tag="VIS73_11170"
     CDS             3607..4299
                     /locus_tag="VIS73_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5763756.1"
                     /translation="MNDVIDYLPAILAGSLVTLKVGLMALVLALALGLVGASAKLSRS
                     RWLSWPATAYTSLVRGVPDLVMMLLVFYGGQVIANELTDALEWPPLEINPFTAGFITI
                     GFIFGAYFTETFRGAFMAVPRGQIEAGQAYGMSRWQVFARVLFPQMLRHALPGLGNNW
                     QVMLKSTAIVSLIGLADMTWLADQAGRTTRQPFVFYLIVCVLYLAMTWVSGRAIGWLD
                     RKYAAGVRQPAS"
     gene            4299..5027
                     /locus_tag="VIS73_11175"
     CDS             4299..5027
                     /locus_tag="VIS73_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5763757.1"
                     /translation="MFDFDVIRESLPQFLSGTWLSIHLVVLSLTAGLAIAIPFAVAAN
                     SPNPWVRRPVATYVYFFRGTPMLVQLLLIYYGLSQFEWMQAAWADGHPFWILFREPYF
                     CAWLAFALNTGAYTTEIIAGSMRHIPHGEIEAAQAIGMTRLTQLRRIVLPGALRRAIP
                     AYSNEVILMLQGSAIASAVTLVDITGAARNTYSRHFAPFESFIFAGLIYMALTLVLVG
                     LFRLAETRWLAHLQPRKPSGAIQP"
     gene            5041..5802
                     /locus_tag="VIS73_11180"
     CDS             5041..5802
                     /locus_tag="VIS73_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5763758.1"
                     /translation="MYQLMVEDLHKRYGDHEVLKGVSLRAKAGDVISIIGSSGSGKST
                     FLRCINFLEHPSAGKITVSGEELRLVSDRHGALVPDAKQLQRVRTELAMVFQHFNLWA
                     HMTAFENVVEAPIHVLKLPRAEAIARAEKYLAQVGIQQWRNSYPAHLSGGQQQRVAIA
                     RALAMEPSVLLFDEPTSALDPELVGEVLKVMRNLADEGRTMVVVTHEMGFAREVSDHV
                     IFLHQGVIEEEGPPAELFGNPKSERLRQFLSGSLK"
     gene            5807..6724
                     /locus_tag="VIS73_11185"
     CDS             5807..6724
                     /locus_tag="VIS73_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5763759.1"
                     /translation="MKRLQADLLLLLAALIWGTSFVAQHTGMRDVGPLLFTGSRFLLG
                     ALVVAPFAWWEWQRLARAGRLPDRRALYGGVALGGLLFLGAIFQQIGIAGTTVSNAGF
                     LTALYVPLVPLIAAMFLGDRVHWIVWPASVGCLAGTYLLGGGSLTALVAGDFWVMLSA
                     LFWAAHVLVVGRVAVRMGTPMGAAFLQFLVCGLLGMVLGSVVETVDAAALLRALPSIA
                     YAGILSVGIGFTLQVVAQRHTPAADAAILLSCETLFAAIAARMFLGETLSAVQIVGGT
                     LIFASVLSIQILPILRFGRAKIMRPRPAA"
     gene            complement(6757..7401)
                     /gene="leuE"
                     /locus_tag="VIS73_11190"
     CDS             complement(6757..7401)
                     /gene="leuE"
                     /locus_tag="VIS73_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HEY5763760.1"
                     /translation="MFYGVTDLATFILGTIFIVLLPGPNSLYVMTVASRSGVGRGYRG
                     ACGIFVGDAILMLLAATGAASLLHASPALFGVIKYAGAGYLAWIGLGLLRSAPALWRG
                     TAHDAERPPDAAGRPFRTALIISLMNPKAILFFVSFFIQFVDPAYPNPVVPFLILGVI
                     VQICSALYLSALIFGGVYLAEQFRRRRRLSGIATGGVGALFMGFGFKLAGATLG"
     gene            7538..8338
                     /locus_tag="VIS73_11195"
     CDS             7538..8338
                     /locus_tag="VIS73_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763761.1"
                     /translation="MIIRCLSLLLLVLSMTVVRAEQSTAIASATATPAGFDLALQFSD
                     YLYDEPTVAVYLDGRQLGLRGAYTHELDGERFARVDLRAAYGAITYHGTGTAKRQPNF
                     IVETRYVLGHDFFPGESLGLAPFVGIGYRFLYNDLRGLTSTGAAGYRRHSHYLYLPLG
                     LTARFVTAGGWIVAPTLEYDHFLRGRQYSHLSDTGIPGFVDVVNTQRDGHGYRASLQF
                     EKGTLSFGPWVHIWRIADSDTVRFSPTMVGREPENNTREIGIEFISRF"
     gene            complement(8363..8803)
                     /locus_tag="VIS73_11200"
     CDS             complement(8363..8803)
                     /locus_tag="VIS73_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763762.1"
                     /translation="MAIEFIPETQLRNATPPFGMGIIRLTAAAAIVMVSTLPTANAEQ
                     RFPDVLAAMVRLSGPERFDFDVTVSSPYDTPQRYADGFRVTDGAGKVLGERKLWHDHA
                     GEQPFTRDLHGVSIPRGIRRVRIQARDQRHGYGGTAIEVELPGR"
     gene            complement(9019..9699)
                     /locus_tag="VIS73_11205"
     CDS             complement(9019..9699)
                     /locus_tag="VIS73_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY5763763.1"
                     /translation="MPTLYIANKNYSSWSLRPWVLMRELGIPFEEKLKPFGLPGSFRS
                     FSPSGKVPCLVEGDTVVWDSLAIVEYLAERHPGVWPADAEARAWARSAAAEMHSGFST
                     MRTIFGMNCGLRVRVDPLPLALRDDLARLEELWGDGLSRFGGPFLAGASFSAVDAFFA
                     PVAFRIQTFQPPLGATASAYAQRLLALPAMQSWYADALAEVWRDEAHDAEAREFGTIT
                     ADLRATAA"
     gene            complement(9731..10237)
                     /locus_tag="VIS73_11210"
     CDS             complement(9731..10237)
                     /locus_tag="VIS73_11210"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY5763764.1"
                     /translation="MGKRDSAVTAAPHRYQAIAAGPDFAIGVLCTDEEIVALHYLPPQ
                     PALAPKNLLAAEALRQIDAYFADPRFQFSLPLRPTGTPFQRRVQAEVARIPSGETRSY
                     GEVARAIRSGARAVGGACGANPFPLAIPCHRVVAAGGALGGVSGRDETSGEWVIAVKR
                     RLLEHERQ"
     gene            complement(10237..11049)
                     /locus_tag="VIS73_11215"
     CDS             complement(10237..11049)
                     /locus_tag="VIS73_11215"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY5763765.1"
                     /translation="MPTPSDLDDIARELKQAQDNSAQITPASDRFPGFDLAAGYAVGE
                     RIHRLRVAEGDLPVGRKVGMTLRSRWPELGVSAPIWAWLRQRSVRRFRQDDASLSLAP
                     FCQPKIEPELVVHFRNAPPAGADEAAMIAAIDWVAPGIEIVQTHYRNGFGKPPDSLAD
                     CAHHAALLIGTPMPVDQLGSEVVGQMTRLSVRLCCGGTLRETGTAENVLGSPLTALCE
                     IATIMAAHGAPLAAGEVVTTGTITTPCPIAVGETWEVTFDGIALPALSVRFE"
     gene            complement(11139..11846)
                     /locus_tag="VIS73_11220"
     CDS             complement(11139..11846)
                     /locus_tag="VIS73_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763766.1"
                     /translation="MEGTTIKTLLLSALLLAAGCDQTTISKPSPPAAASAQVTGSACV
                     IDERWLQHDYDHAVDYANPGVRTDFFLLVYSHSPSFCQRMAERGRHDETLLQCRSPNR
                     FGWVIHGLWGESESAYRRREDRSHPRFCKGDLPPLPLEAIKPYLCMSPGTRLLQGQWE
                     KHGACDFDSAGAYFDKTKALFERFPVPPGELKAGAAVSWMKDNSPALRDKWLHRSGDE
                     FGICFSTAFEVISCPRR"
     gene            complement(12055..12471)
                     /locus_tag="VIS73_11225"
     CDS             complement(12055..12471)
                     /locus_tag="VIS73_11225"
                     /inference="COORDINATES: protein motif:HMM:NF020479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HEY5763767.1"
                     /translation="MSLCFAAGALTAVLNIQAFTLAWTHSIEKIRWEEDWRIENGKLV
                     VVEARIRGTGAGMEPPDGAKLIDGVWHYRPALAPLESVTLAHSPYAKPYELCVAGLCR
                     PLTAALPGIGASAPIVLSPCTAPSQASASPAANTRR"
     gene            complement(12468..14507)
                     /locus_tag="VIS73_11230"
     CDS             complement(12468..14507)
                     /locus_tag="VIS73_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEY5763768.1"
                     /translation="MSELRLAEFGWTQGREARLLFLIAIAFSAFQIVTAAFSPLSSQI
                     VRAVHVGFLLLLIFGVFQGWGPTGKARPLHAWPLAATGFVLAFYHWIFEADLIQRAGD
                     PTIADIVVGTITIALVFEAARRVMGIALPLICGIFLAYALFGNHLPGTLAHRGYDFMQ
                     VVEHLGFGTEGIYGVPTYVSSSYIFLFILFGSFLEKAGMIQLFTDFAMGTVGHTKGGP
                     AKVSVVASGLMGTINGSGVANVVTTGQFTIPLMKRFGYKPEFAGGVEATSSMGGQIMP
                     PVMGAVAFIMAETINVPYVEVAKAATIPAMLYFITAFWMVHLEAGRRHLSGLAKADCP
                     DPWAAVKKRWYLLLPLIVLVWLLFSGYTPLFSGTVGLALTAILIFGAAIAMRLQGNAL
                     RVAFWIGLGILCSSFVKWGIVAYFVLIAALVAGCALVHGGRGTLTVAVNALADGARHA
                     LPVGIACALVGIIIGVLTLTGAATTLASAIIAVGEGSLLASLMLTMLVCLVLGMGIPT
                     IPNYIITSSLAAPALLELGVPLIVSHMFVFYFGILADLTPPVALAAFAAAPIAGVSGM
                     KIGVQAVRVAIAGFVVPYMAVYAPELMLQGDWTIASTGYVIFKALVAIGLWGAASIGY
                     LFAPLGWFHRVWAGVSAALLIAALPATDELGLGGAALFIAWALWQQRRAAKPASA"
     gene            complement(14579..>15256)
                     /locus_tag="VIS73_11235"
     CDS             complement(14579..>15256)
                     /locus_tag="VIS73_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY5763769.1"
                     /translation="WKGNADAGFKAKLDKLRGLAATYPNYIQIVASADSGIKSLTDLK
                     GKRVAVGAPKSGTELNARAIFKGAGMSYNDFSKTEYLPFGESVELLKNRQLDATLISA
                     GLGVAAIRDLATAVKIVIVTIPAEVTTKIGDAAYQPATVPANTYDGQTADVQTIAIRN
                     FLATHSGVSDDAVYAMTKAMFDNLGDLQAAHAAAKGISKENAAAGMPLPLHPGAEKYY
                     REQGVLK"
BASE COUNT         2640 a         4717 c         5153 g         2746 t
ORIGIN      
        1 accgcgccga gcagaattcc gagcgtgatg ccgttcaaga cgccgacctt gatctcctga
       61 atccaaaccc gcagcacctc gtagggtttg acgatgccca gcgagagctc gcgcatgctg
      121 acggcgacgg cctggtttcc cgagcagccg ctcatgtccg agacgatggg cagaaacacg
      181 gccagcgcaa tcgctgcact cagcgtgtcg aaatacatgg cgatcacgct ggcggcgacg
      241 atgttgagca cgatgttcag gctcagccag gacaggcgcc gccccgcacg cgtgagcacc
      301 ggcagcgagc ggatctcgtc gccgccgatg atgccctttg acttggcgta ttcctcctgc
      361 gagcggatgg cgatggcctc ggccacctcg tcgcggccga ctacgccaac cagttcctgg
      421 tgtgcgtcga tgaccggcac cgcctggaaa tcgtagcggt cgaaaaacgt ctccagctcg
      481 tcgagcgtcg ccccggcgcg tacgaacagc gatgacttga ggatcgaagc cagcggcgtg
      541 gtccgccgtg cgaggactag gtcgcgcaga tcgagcacgc cgatgagctt gccggtggtg
      601 gagaccacat atacgtgggc atcgtgttcc ggctcctcac gctcgtcggc gcgtttgctg
      661 agatcgtcga taacatcgcc gaccagcgcg gactcgccgt aggcgaggta gtcggtcgac
      721 atcagtccac cggcgacatc cgggtcgtag cggatcaggc gacggacgtg cgcggcgtcc
      781 tccggggcca tctcggcgag aatcgcctcg gcatcgtcgt cgtgcatgtc ggcgatgacg
      841 tcgacctggt cgtcgacgtc catctcactc aggatcgatg ccgcctgctt ggccgggagg
      901 ctttcgatca ggtcggaggc gtgctcgtcg gggatttcct cgatgagatc ggcggcctcc
      961 tcaggcgaca gcgtggtcag gaccttctcc cgatgttcgg gtgcgagtcg gagcagcgcg
     1021 cgaaatgcct cgctggggcc gatgctgtcg acgtaggcct ccaatgtctt gctgtcgccg
     1081 gcggtggcga gtttctcaag cagttcccag gtcttcggtg actgctcgcg cgattcggtc
     1141 gtgtcttcga ttgccattgc tgtctgctcc acctggacgt tcgcgccgcg cgcgacatgc
     1201 ccgctcgtat ttactatggg tcgccacgaa aagataggga cgccgcgagc gacagcgcgt
     1261 gttgcgtttc gtggaaggca ggcaagaaaa aacccgctgc cgaaacgaca gcgggtctag
     1321 atcgcgaaaa ccggcgatgc gacagccggt ttggcgggtg cagtttagaa cacgaagatc
     1381 acgccgacgg cgtaggagtt cagatcgaca gcgggcgcct tgttgtcgag gcgcagtgct
     1441 tcggcgtagg cataggtgtt cttgaagatc tcgcggttga tgaacagttc gagggccttg
     1501 gccttggtgt ccttgttctg ggcaacgtgg gcacccatcg tgaaaccggc aaccggggcc
     1561 gtcacgccca gcgtcgggcc agtggcgttg agaccggcgt cggtgtagcc cgccattgcc
     1621 ttgaggaacc cgaagtcgta gctggctgcg accgagttga agtcttcgcc attcagcgtc
     1681 ttcttgccct catgcgaggc gccgatcacc aggttcttga tgccgctgta ggtaacggca
     1741 gcggatgcga cgccacgggt gttgtcgtcg taggtggagg cgtcgcggag gtcagtgttg
     1801 tcaccacgcg ggacgtagcc aacctgcgcc ttgaagccgc tcatttcggg cgagatgtac
     1861 tgcaccgagc gcgactggcg atcggtcgcc cagacgccaa cgccggaata ggccgaggtg
     1921 gcaccgttgg actggccgtt gaagtcgaat gcgcccatca cctggaaggg gatcgagtcc
     1981 tggcgaccga cgcgaatctc gcccatcttg gtgtgcgaga agccgagcca cgcctgacga
     2041 ttgaagaacg ccgggccgtt gaccgcgcca ttgctggtga tgccgttggt ctcgaacttg
     2101 aagtgggcct tgacgtcctt gtcgacgtcg aagcttccct tgacgccgat acgcgtcgtc
     2161 gagttgccct gcgattggct atcgtcaccg cccgagtgga tgtcggtctt agcggtcttg
     2221 ccgtcgacga cttcgttctt gccgacgctc atgtcgatga ggccgtagat cgaaacctgg
     2281 gcttgggcgg caccggcgaa ggcgagtgca atcgcggccg ggacgatctt cttcatgaag
     2341 ttcattgtca tctctctcga attggttgct gggttggtcc atacgcgccc gtattggggc
     2401 gagcgcggat tatccacgag tcagcgatgg ctggcgtgag gcgcgcaaat ttgttcaatc
     2461 tgtggtggca taagtgttgc aaatttgcca ctgatatcgt ttcaaacaga atttgcggtg
     2521 aggtgcgcag acagggaggt tcccgccttg gcaggacacc ggccttcttt gggcgttgag
     2581 gccgggtagc gcttctgtca tgattcgcgc cttgccgagg tgcccgctgg ccacgcccgc
     2641 gaactggagc ggtggttttc cggacggaac ggcgtaaaaa gatctctgga gaagacatga
     2701 agaaaatcgt cctcgccggc ctcgtgctgg catttgccac cggtttcgcg caggccaagg
     2761 aatggaagca gattcgtctg gcaaccgagg gtgcctatcc cccgttcaac gaaacggcgc
     2821 cagacggctc ggtgcgcggc ttcgacatcg acatcggcaa tgcggtctgt gccgagatga
     2881 aggcgaagtg cagctgggtc aagcaggagt gggacggcat gattccagcg ctgttgtcgc
     2941 gcaagttcga cgcaatcatc gcctcgatgt cgatcaccga agaacgcaag aagaaagtcg
     3001 atttttccga caagtactac acgacgccgc tcgcgctggt cgccaaggaa ggcagcccgt
     3061 tggtgccgac cgccgaatcg ctcaagggca agaaggtcgg cgtccagcgc ggcacgatcg
     3121 ccgacaactt cgcgaccaag ttttgggagc cgaagggcgt gagcgtggtg cgctacgcca
     3181 agcaggaaga ggcctatcta gaccttggtg ccggccgcat ggatgcgggt ttttccgaca
     3241 tgctcgaagc cgagggtggg ttcatcagca agcctgaggg caagggtttc gccttcgtcg
     3301 gcaacaaggt cttcggcaag acggctgagg agaaggccgt catcggcgag ggcatcggca
     3361 tcgccgtgcg caagaaggac gcggagctca aagccgcctt taacaaggcc attgccgcta
     3421 ttcgcgccaa cggtacttac aacacgattc gcgccaagta cttcaagtac gacatctacg
     3481 gcgagtgatt tccgctcgcc ttaccggcgg ggctgtggtc ttagtgccgc gtccgccggt
     3541 gccaagaatc tcccggcggg gcagcggcct tagtgctgcg tccgccggcg caaggattac
     3601 cgcagtttga acgacgtaat cgactacctc ccggccatcc tcgccggcag cctcgtcacg
     3661 cttaaggttg gactgatggc gctcgtgctg gcactggcac tggggctggt cggggcttcg
     3721 gcgaagcttt cgcgctcgcg ctggctgtct tggcctgcga cggcctacac ctcgctggtt
     3781 cgtggcgtgc ccgacctggt gatgatgctg ctggttttct acggcgggca ggtgatcgcc
     3841 aacgagctga ccgatgcgct cgagtggccg ccgctcgaaa tcaacccgtt taccgctggc
     3901 ttcatcacca tcggcttcat cttcggcgcc tatttcaccg agacctttcg cggcgccttc
     3961 atggccgtgc cgcgcgggca gatcgaggcc gggcaggcct acgggatgtc tcgctggcag
     4021 gtattcgcgc gcgtgctgtt tccgcagatg ctgcggcatg cgctgccggg actcggcaac
     4081 aattggcagg tcatgctcaa gagcacggca atcgtttcgc tgatcgggct cgccgacatg
     4141 acctggctgg ccgatcaggc cgggcgaacg acgcgccagc cctttgtctt ctacctcatc
     4201 gtctgcgtcc tctatctggc gatgacctgg gtttccggtc gggcgatcgg ctggctcgat
     4261 cgcaagtacg cggcgggtgt ccgccagccg gcaagctgat gtttgacttc gacgtcatcc
     4321 gcgaaagcct gccgcagttt ctcagcggca cctggctgtc aatccatctg gtcgttctct
     4381 cgctgactgc cggactggcg atcgccatcc ccttcgccgt cgccgccaat tcgccgaacc
     4441 cttgggtgcg ccgtccggtg gcgacctacg tctatttctt ccgtggcacg ccgatgctgg
     4501 tccagctgct gctgatctac tacgggttgt cgcagttcga gtggatgcag gcggcctggg
     4561 ccgacgggca tccgttctgg attctgttcc gcgagccgta tttctgcgcc tggctggcct
     4621 tcgcgctgaa caccggcgcc tataccaccg agatcatcgc cggctcgatg cggcatattc
     4681 cgcacggcga gatcgaggcg gcgcaggcga taggcatgac gcgcctgacg cagctgcgcc
     4741 gcatcgtcct gccgggtgcc ttgcgtcgcg cgattccggc ctacagcaac gaggtgatcc
     4801 tcatgctcca gggcagcgcg atcgccagcg cggtgacgct ggtcgacatc accggtgcag
     4861 cgcgcaatac ctactcgcgc catttcgcgc catttgaaag cttcatcttc gccggcctga
     4921 tctacatggc cctgacgctg gtgttggtcg gcctgttccg cctcgcggag acgcgctggc
     4981 tggcccacct gcagccgcgc aagccgagcg gcgcgatcca accctgagaa gagaattcgc
     5041 atgtaccaac tgatggtcga ggacctgcac aagcgctacg gtgaccacga ggtgctcaag
     5101 ggcgtttcat tgcgcgccaa ggccggcgac gtgatcagca tcatcggctc gtcgggctcg
     5161 ggcaaaagca ccttcctgcg ctgcattaac ttcctcgagc acccgagcgc tgggaagatc
     5221 acggtcagtg gagaggagct gcgcctggtg tcggatcgac acggcgcgct ggtccccgac
     5281 gccaagcagt tgcagcgcgt gcgcaccgag ctggcgatgg tttttcagca tttcaacctg
     5341 tgggcgcaca tgaccgcgtt tgagaacgtc gtcgaggcgc cgatccacgt gctcaagttg
     5401 ccgcgcgccg aggcgatcgc ccgtgccgaa aagtatctcg cgcaggtcgg catccagcag
     5461 tggcgcaaca gctatccggc gcatctctcg ggcggccagc agcagcgcgt ggcgattgcc
     5521 cgggcgctgg cgatggagcc gtctgtcctg cttttcgacg agccgacctc ggcgctcgac
     5581 cccgaactcg tcggcgaagt gctcaaggtc atgcgcaatc tcgccgacga ggggcggacg
     5641 atggtcgtcg tcacccacga gatgggtttt gcgcgtgagg tgtccgatca tgtgatcttc
     5701 ctacaccaag gcgtgatcga agaggagggg ccgccggccg agctgttcgg caatccgaag
     5761 agcgagcggc tgcgccagtt cctgtcgggc agcctgaagt aagcccatga agcgtctgca
     5821 ggccgacctg ttgctgctgc tcgcagcact gatctggggc acgtcctttg tcgcccagca
     5881 caccggcatg cgcgacgtcg ggccgctcct gtttaccggc agccgcttcc tgctcggggc
     5941 gctggtcgtc gcaccttttg cctggtggga gtggcagcgc cttgctcgcg ctggccgcct
     6001 gcccgaccgt cgcgcgctgt acggtggggt cgcgctcggc ggtctgcttt ttctcggggc
     6061 gatcttccag cagatcggca ttgccggcac gacggtcagc aatgcgggtt ttctcaccgc
     6121 gctgtacgtg ccgctggtgc cgctgattgc cgcgatgttt ctcggcgatc gcgtgcactg
     6181 gatcgtctgg ccggccagcg tcggttgcct cgccggcacc tacctactcg gcggcggctc
     6241 gctgacggcg ctggtggcgg gcgatttctg ggtgatgctc agtgcgctgt tctgggccgc
     6301 tcacgtgctc gtcgtggggc gcgttgccgt gcggatgggg acgccgatgg gcgctgcctt
     6361 cctgcagttt ttggtctgtg gcctgctcgg catggtgcta ggcagcgtcg tcgaaactgt
     6421 cgatgcggcg gcgctgctgc gtgcgctgcc atcgatcgcc tacgccggaa tcctttcagt
     6481 cggcatcggt ttcacgctgc aggtcgtcgc gcagcgccac acgccggccg ccgatgcggc
     6541 aattctgctc agttgcgaga cgctgttcgc ggcaatcgcg gcacggatgt tcctcggcga
     6601 aacgctgtcg gccgtgcaga ttgtcggtgg cacgctgatt ttcgcctcgg tgctgtcgat
     6661 ccagatcctg ccgattctgc gctttggtcg cgcgaagatc atgcggccga gacccgccgc
     6721 ctaagcgtac cgggcgtagc ccggcgacgc catcgatcag ccgagtgttg cgcccgccaa
     6781 cttgaagccg aagcccatga acagggcgcc cacgccgccg gtagcgatgc ccgagaggcg
     6841 ccgacgccgg cggaactgct cggctagata gaccccgccg aagatcagcg ccgagagata
     6901 gagcgcgctg cagatctgca cgatcacgcc gaggatcagg aagggaacga ccgggttcgg
     6961 gtaggctggg tcgacgaact ggatgaagaa ggagacgaaa aagaggatcg ccttcggatt
     7021 catcaggctg atgatcaacg ccgtgcgaaa cggccggccg gcagcgtcgg gcggacgctc
     7081 ggcgtcgtgc gcggtgccgc gccagagcgc cggggcgctg cgcagcagac caagcccaat
     7141 ccaggcgaga tagccggcgc cggcgtactt gatgacgccg aacagggccg gactggcgtg
     7201 gagcagcgag gccgcgccgg tcgccgcgag cagcatcagg atcgcatcgc cgacgaagat
     7261 gccgcaggcg ccgcgatagc cgcggccgac gccggagcgc gaagcgacgg tcatcacgta
     7321 gagcgagttc ggtccgggca gcagtacgat gaagatcgtg ccgaggatga aagtcgcgag
     7381 atcggttaca ccgtagaaca tcgggctctg ctcaggaaaa gtggcgcggc tggtagcgcg
     7441 cctgccggga cggcagcgag tttccgccgc cgtggaatga tgcccgaaac cggcactatt
     7501 tcgccagtat tggcgatgga cagcctagac tcgcgccatg atcattcgtt gcctgtcact
     7561 gctgctcctg gtgttgtcaa tgaccgtcgt ccgggccgag caatcgaccg caattgcgag
     7621 tgcaaccgcg acgccggccg gcttcgacct cgcgctgcag ttctccgact acctgtacga
     7681 cgagccgacg gttgcggtct acctcgacgg ccggcagctg ggcctgcgtg gcgcctatac
     7741 gcatgagctt gacggcgagc gtttcgcccg cgtcgatctg cgggctgctt acggtgcgat
     7801 cacctaccac ggcaccggca cggccaagcg acagccgaac ttcatcgtcg agacgcgcta
     7861 cgtgctggga catgactttt tccccggcga atcgctcggc ctcgccccct ttgtcggcat
     7921 cggctaccgc ttcctctaca acgatctgcg cggcttgacc agcacaggcg cggccggcta
     7981 ccggcgccat tcgcactacc tttatttgcc gctaggcctg acggcgcgat ttgtcacggc
     8041 gggtggctgg atcgtcgcgc cgacgcttga gtacgaccac tttctccgcg gccggcagta
     8101 ctcgcatctg agcgacacgg gcataccggg tttcgtcgat gtcgtgaata cccagcgtga
     8161 cggacacggc tatcgcgcat cgctgcagtt cgagaaaggc accttgagct tcgggccgtg
     8221 ggtgcacatc tggcggattg ccgattctga caccgtgcgt ttttcgccga cgatggttgg
     8281 gcgcgaaccg gagaacaaca cgcgggaaat cggcatcgaa ttcatctcgc gcttctagct
     8341 tgtcggtccg agtcgcgccc ggctaccgac ccggcaactc gacttcgatc gcagtaccgc
     8401 cgtagccatg gcgctggtca cgtgcctgaa tgcgtaccct gcggattccg cgagggatcg
     8461 ataccccatg caggtcgcgc gtaaatggtt gttcaccggc gtggtcgtgc cagagcttac
     8521 gttcgccgag gaccttgccc gcgccatcgg tgacgcgaaa gccgtcggcg tagcgctgcg
     8581 gtgtgtcgta gggtgaagac accgtgacgt cgaagtcgaa acgttcaggg ccgctcagcc
     8641 ggaccatcgc agcgaggaca tcgggaaagc gctgctcggc attcgcggtc ggtagggtgc
     8701 tcaccatcac gatcgcagca gctgcggtta agcggatgat gcccatgccg aatggcggcg
     8761 tcgcgttgcg gagttgcgtt tcagggatga attcgatcgc catcggtgct cccaattagc
     8821 ttgttctttg gagtcgatcc gcatcgccac cgccttttcg cgcttgcacc agctattgcg
     8881 gacctgggcc gagcgtgatc gagcccgccg cgaattgcgc aacctcgaga agccaccctg
     8941 cgcgatgtcg gcatcagtcg cgccgatgca gatttcgaag gcggcgagcc gttctggcgg
     9001 gaatgagcgg attcgctgct acgccgcggt cgcgcgcagg tcggcggtga tcgtgccgaa
     9061 ctcgcgggcc tcggcatcgt gcgcttcgtc gcgccacacc tcggccagcg cgtcggcgta
     9121 ccacgattgc attgccggca gagctagcag ccgctgcgcg taggcactgg cggttgcccc
     9181 aaggggtggc tggaaggtct gaatgcggaa tgccaccggc gcgaagaagg cgtcgactgc
     9241 gctgaagctc gcgccggcga ggaaaggtcc gccgaaccgc gacaagccgt cgccccacaa
     9301 ttcctcgagg cgtgcgaggt cgtcgcgcag cgccagcggc aaggggtcga cgcgcacccg
     9361 cagtccgcag ttcatgccga agatcgtgcg catcgtcgag aagcccgaat gcatctcggc
     9421 cgctgccgag cgcgcccagg cccgcgcctc cgcatcggca ggccagacgc cggggtgacg
     9481 ctcggcgagg tactcgacga tagcgagtga atcccagacc acggtgtcgc cctcgacgag
     9541 gcagggcacc ttgcccgacg gcgaaaagct gcggaacgat ccgggtaagc cgaatggctt
     9601 gagcttttcc tcgaagggaa tgcccagttc gcgcatcagc acccaggggc gcagcgacca
     9661 cgacgagtag ttcttgttgg cgatgtagag cgtcggcatg ggtttctcct agcgtgatct
     9721 ttggcggcga ttattgccgt tcgtgctcga gcagacggcg cttcacggcg atcacccact
     9781 cgccgctggt ttcgtccctg ccgctgacgc cgccaagggc cccgccggcg gcgacgaccc
     9841 ggtggcaggg aatcgctagc ggaaacggat tggcaccgca ggcgccgccc accgctcgcg
     9901 ctccgctgcg gattgcgcgg gcgacctcgc cgtagctgcg cgtctcgccg ctggggatgc
     9961 gcgcgacctc ggcctgcacg cggcgctgga agggcgtgcc ggtcgggcgc agcggcaacg
    10021 agaactggaa acgcggatcg gcgaaatagg catcgatttg gcgcagtgct tcggcggcca
    10081 gcaggttttt cggggccagc gcgggctgcg gtggcaggta gtgcagggcg acaatctcct
    10141 cgtctgtgca cagcacgccg atcgcgaagt ccgggccggc ggcaatggcc tggtagcggt
    10201 gcggtgccgc tgttacggcg ctgtcgcgct tgcccattac tcgaagcgga ccgacagcgc
    10261 gggcagcgcg atgccgtcga aggtcacctc ccaggtctcg ccgacggcga tcgggcaggg
    10321 cgtggtgatc gtgccggtgg tcaccacttc gcccgccgcg agcggcgcgc cgtgggcggc
    10381 catgatcgtc gcgatctcac agagcgccgt gagcgggctg ccgagcacgt tttccgccgt
    10441 gccggtttcg cgcagcgtgc cgccgcagca gaggcgcacc gacagccgtg tcatctgtcc
    10501 gacgacctcg ctccccagtt ggtcgaccgg catcggcgtg ccgatgagca gcgccgcgtg
    10561 gtgcgcgcaa tcggccagcg aatcgggcgg cttgccgaag ccgttgcggt aatgtgtctg
    10621 cacgatctcg atgcccggcg cgacccagtc gatcgcagcg atcatggccg cctcgtcggc
    10681 acccgccggc ggcgcattgc ggaaatgcac caccagctcg ggttcgatct tcggctggca
    10741 aaagggtgcc aggctgaggc tcgcgtcgtc ttgccgaaaa cggcggacgg agcgctggcg
    10801 cagccaggcc cagatcggcg ccgaaacgcc cagttccggc cagcgtgacc gcagcgtcat
    10861 gccaaccttg cggccgaccg gcaggtcacc ctcggccacg cgcaggcggt gaattcgctc
    10921 gcctaccgcg tagccggcgg ccaggtcgaa gcccggaaaa cgatcggatg ctggcgtgat
    10981 ctgcgccgaa ttgtcctgcg cctgcttgag ttcgcgggca atgtcgtcga ggtctgaagg
    11041 ggtcggcacg ggttctggct cgctgaagta ggtcatcaat ggagcaaatc gctggtgtcg
    11101 agtctaactt tgcccacggg gtatcggccg gcaatcgatc agcggcgcgg acacgagatc
    11161 acttcgaagg ccgtggaaaa gcaaatcccg aattcgtcgc ccgaacgatg cagccacttg
    11221 tcgcggagcg ccgggctatt gtccttcatc cagctcacgg cggcgccggc ctttaactca
    11281 ccgggtggca cggggaatcg ctcgaacaag gccttcgtct tgtcgaaata ggcgccggcg
    11341 gagtcaaagt cacaggcgcc atgcttttcc cattggccct gcagcaggcg agtgccgggg
    11401 ctcatgcaca ggtagggttt gatcgcctca agtggcagcg gcggaagatc gcccttgcag
    11461 aagcgcggat gactgcggtc ctcgcggcgg cggtaggcgc tctcggactc gccccacaag
    11521 ccgtggatga cccagccgaa acgattcgga gaccggcact gcaggagcgt ctcatcgtgg
    11581 cggccacgct cggccatccg ctggcaaaac gacggcgaat gcgagtacac gagaaggaag
    11641 aagtctgtcc tgacgccggg attggcgtaa tcgacggcat ggtcgtagtc gtgctgcagc
    11701 catcgctcgt cgattacgca agcgctaccg gtgacctgcg cgctggcagc agcgggcggc
    11761 gatggcttcg atattgtggt ctggtcgcag cctgccgcaa gcaagagagc agatagcagt
    11821 agcgtcttga tcgtagtgcc ttccatttca gcaatattct gttgaacttg ccggaaactc
    11881 gtgcgtagtt tcttttcaaa tccgcggcgc gtgcgggggg cccccggctt ctcgggcggg
    11941 cgaagcaatc gacgccatga tcgacgaaaa cgcatggtgg ttagccgcga cgacaccgag
    12001 taggcgaacg cgttgggcgc aggctgagct tcgaaggcca gtctgccgcg gcccttatcg
    12061 ccgcgtgttg gcggccgggc ttgctgaagc ctgactcgga gcggtgcagg gcgacaacac
    12121 gattggcgcg ctcgcgccga ttccggggag ggcagccgta agggggcggc agaggccggc
    12181 tacgcatagc tcgtagggct ttgcatacgg cgaatgcgcc agcgtcaccg attccagagg
    12241 cgcgagtgcc ggccggtagt gccagacgcc gtcgatgagt ttcgcgccat cgggcggctc
    12301 catgccagcg ccggtgccgc ggattcgggc ttcgacgacg accagcttgc cgttctcgat
    12361 ccgccagtct tcttcccagc ggatcttttc gatcgagtgc gtccaggcga gcgtgaatgc
    12421 ctggatgtta agtaccgccg tcagtgcccc ggcggcaaag cagaggctca tgccgacgcc
    12481 ggcttggcgg cgcgccgttg ctgccagagc gcccaggcga tgaagagcgc ggcaccgccg
    12541 agacccagct cgtcggtggc gggcagggcg gcaatcagca gcgcggccga gacaccggcc
    12601 cacacgcgat ggaaccagcc gagtggcgcg aacaggtagc cgatcgacgc ggcgccccag
    12661 aggccgatgg cgaccagcgc cttgaagatg acataaccgg tcgaggcgat ggtccagtca
    12721 ccctgcagca ttagttcggg cgcgtagacg gccatgtagg gcacgacaaa gccagcgatg
    12781 gcgacacgaa cggcctgaac gccgatcttc atgcccgaga cgccggctat cggcgccgcg
    12841 gcgaaggcgg cgagcgcaac cggcggtgtc aggtcggcga ggatgccaaa gtagaagacg
    12901 aacatgtggc tgacgatcag cggcaccccg agttcgagca gcgccggtgc ggcgagcgag
    12961 gaggtgatga tgtagttggg gatggtcggg atgcccatgc cgagcacgag gcagacgagc
    13021 atggtcagca tgagggaagc cagcaggctg ccctcgccga cggcgatgat cgccgaggcg
    13081 agcgttgtcg cggcgccggt gagcgtgagc acgccgatga tgatgccgac cagcgcgcag
    13141 gcgatgccaa ctggcagtgc gtggcgcgcc ccgtcggcaa gcgcgttgac ggcaacggtc
    13201 agcgtgccac gcccaccatg gaccagtgca caaccggcga ccagcgcggc gatcaatacg
    13261 aagtaggcga cgatgcccca cttcacgaag gaggagcaga gaatgccgag gccgatccag
    13321 aaggcgacgc gcaacgcatt gccctgcagg cgcatcgcga tcgccgcacc gaagatcagg
    13381 atcgcggtga gcgccaggcc cacggttccg gaaaagagcg gcgtgtagcc ggagaagagc
    13441 agccagacca gcacgatcag cggcagcagc aggtaccagc gctttttgac cgcggcccag
    13501 gggtccgggc agtcggcctt ggcgaggccc gagagatgcc gccggccggc ttcgagatgg
    13561 accatccaga aggcggtgat gaagtagagc atcgccggga tcgtcgccgc cttagcgacc
    13621 tcgacgtagg gcacgttgat tgtctcggcc atgatgaagg cgacggcgcc catcaccggc
    13681 ggcatgatct ggccacccat cgaagaggtg gcctcgacgc cgccggcgaa ctccggcttg
    13741 tagccgaatc gcttcatcag tgggatcgtg aactggccgg tggtgacgac gttggcgaca
    13801 cccgagccgt tgatcgtgcc catcaacccc gaggcgacga ccgatacctt ggccgggccg
    13861 cccttggtat ggccgacggt gcccatcgcg aaatcggtga acagctggat catcccggcc
    13921 ttctcaagga aagagccgaa gaggatgaag aggaagatgt agctcgacga cacataggtc
    13981 ggcacgccat agatgccttc ggtgccgaag cccaggtgct cgacgacctg catgaagtcg
    14041 tagccgcggt gggcgagcgt tccgggcaag tggttgccga acagtgcgta ggccaggaag
    14101 atgccacaga tcagcggtag cgcgatgccc atcacgcgcc gggccgcttc gaagaccagc
    14161 gcaatcgtga tcgtgccgac gacgatgtcg gcgatggtcg ggtcgccggc acgctggatc
    14221 aggtcggcct cgaaaatcca gtggtagaag gcgaggacga agccggttgc ggcaagcggc
    14281 caggcgtgca gtggccgcgc cttgccggtt gggccccaac cctggaagac accgaagatc
    14341 agcagcagca ggaagccgac gtgaacggcg cggacgatct gcgaggaaag cgggctgaac
    14401 gcggcagtga cgatctggaa cgccgaaaag gcgatcgcga tcaggaacag gaggcgcgcc
    14461 tcccgcccct gggtccagcc aaactcggca agccgcagct cagacatttt gcgtcctttc
    14521 ctgccccgaa aaaagaaccg gcagtgggtg ccggttctac tttctcacgg actacggttt
    14581 acttgaggac gccctgttcg cggtaatact tttcggcgcc cggatgcagc ggcagcggca
    14641 tgccggctgc ggcgttttcc ttgctgatgc ccttggccgc ggcgtgcgca gcctgcaggt
    14701 cgccgaggtt gtcgaacatc gccttggtca tcgcgtagac cgcgtcgtcg gaaacgcccg
    14761 agtgcgtcgc gaggaagttg cggatcgcga tcgtctgcac atcggcggtc tggccgtcgt
    14821 aggtgttggc gggcacggtg gccggctggt aggcggcatc gccgatcttg gtggtgacct
    14881 cggcgggaat cgtgacgatg acgatcttca ccgcggttgc gaggtcgcgg attgccgcga
    14941 cgccgaggcc ggcggagatc agcgtggcgt cgagctggcg gttcttcagc agttcgaccg
    15001 actcgccgaa gggcaggtac tcggtcttcg agaagtcgtt atagctcatg ccggcgccct
    15061 tgaagatggc ccgggcgttg agttcggtgc ccgacttcgg cgcgccgacg gcgacgcgct
    15121 tgcccttgag gtcagtgagc gacttgatgc ccgaatcggc cgaggcgacg atctggatgt
    15181 agttcgggta ggtcgcggcg agcccgcgca gcttgtcgag cttggccttg aagccggcgt
    15241 cggcgttgcc cttcca
//
LOCUS       DATDAA010000376         4425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_61832,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000376 DATDAA010000000
VERSION     DATDAA010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61832"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1145)
                     /locus_tag="VIS73_11240"
     CDS             complement(150..1145)
                     /locus_tag="VIS73_11240"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY5763770.1"
                     /translation="MKKLIGLALSTAALMLAAQGAHANDCDKGERVVKLSHVTGGTTH
                     PKVVAANDFAARVNKEMNGSLCVEVYPNSTLFGDSKELEALLLGDVQLLAPSLSKFGS
                     YTKKLGVFDLPFIFKDMDAAIRFTNSPTGKKLLNSMEDVGFVGLGYWMSGMKYFSANK
                     PLLTPADAKGLKFRVQTSDVAKAMIEAMGASPQPMAFAEVYGALQTGVVDGQENTWSN
                     IYTKKFFEVQDGVTETNHQLLAYLFMTTKDFLDSLKPDVRAKFLKIADDVTQETNESV
                     KQAELKNRQNIIDSGGKVRPLTAAQRKAWVDAMKPVWQQFEGEIGKDIIDAAAGS"
     gene            1584..2669
                     /locus_tag="VIS73_11245"
     CDS             1584..2669
                     /locus_tag="VIS73_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY5763771.1"
                     /translation="MAAGLVGLRLAWLGAGFASVILGFAKAVFGHGYNGSMPMPQADL
                     ALARAGEVQFSAKPVIVFGEFLADCFPDRKVAGGAPFNVACHLQGLGASPLLATRIGQ
                     DAGSHFLLDAMKRCALDVALLQFDPLRASGQVDVIVEGGGHRFEIAPDQAFDFIDADP
                     LLAAVDCLDAKRQSPSLIYFGTLAQRGRGSREALRRLLDRCDAPRFVDLNLRPPWVSR
                     DVCGYALTQAKLVKMSEAELRTVAGWLGEDCQKSEGEDLGRRVATRYGIDRLFVTRGE
                     QGAWCVSGGEVIRVSSPAPPSADGDAVGAGDAFSAVLIVGTLGGWPLSLMLERAAHFA
                     SAVCGIRGATPASSDFYRPFLDNWNTR"
     gene            2666..>4425
                     /locus_tag="VIS73_11250"
     CDS             2666..>4425
                     /locus_tag="VIS73_11250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5763772.1"
                     /translation="MTRASRQSPLHIMLINVHGLLRGHNPELGRDSDTGGQIVYVLEL
                     AKALDRDPAVGKIDVLTRQINDPEVDGDYRVAEESLGEKTTLLRLPCGPEKYLRKELL
                     WPHLDQMVDRCLVLLRERGRLPDLIHSHYADAGYVGRQLSLLLGIPQIHTGHSLGRPK
                     RARLLASGRKAEAIDRQYHFDRRIDAEEDVLYNASLVITSTRQEIDEQYGLYRNSDRP
                     RFAVIPPGTDTERFSAPGRRKLSERLLAQIDRFLSDHRKPIILTIARADARKNLGGLL
                     AAFAGDRELRERANLVIVAGSRANIREMDSAQSRVLQDLLLDIDLHDLWGSVAIPKEI
                     AQEDIPDLYRLVARRHGVFVNSALTEPFGLTLIEAAASGLPIIAPDDGGPRDIIANCR
                     NGVLVDTLSPEAIASALKSTLGDQRQLETWAANGLAAVQRHYSWAAHTKRYLKEVRDV
                     MQRERKQARRRHQVWSGGKSTLPLVRDILISDIDNTLIGDGDGLSELTAWLQARRGQV
                     GFGIATGRTIDSTLKILKKWRVGVPDVLVTAVGSEIYYGPGCIADTGWSSHIRHLWRR
                     DELATAMTALPGLELQAEAD"
BASE COUNT          761 a         1344 c         1427 g          893 t
ORIGIN      
        1 gctcggacga cagcttcact gcggggccga ttgtcagcgc gccagcactg cgcgcctact
       61 atcggcgccg cccgcaggcc gtaaacccgg tccggacagc gagcggaaga tcgggcgacg
      121 cgggaaccca cgccgcccgt cgtacagact caggagccgg cagcggcgtc gatgatgtcc
      181 ttgccgatct cgccttcgaa ctgctgccac accggcttca tcgcgtcgac ccaggccttg
      241 cgctgtgccg cggtcagcgg gcggaccttg ccacccgagt cgatgatgtt ctggcggttc
      301 ttcagttcgg cctgcttcac gctctcgttg gtttcctgcg tgacgtcgtc ggcaatcttg
      361 aggaacttgg cgcggacgtc aggcttcagg ctgtcgagga agtccttggt ggtcatgaac
      421 aggtaggcga gcaactggtg gttggtctcg gtgacgccgt cctgcacttc gaagaacttc
      481 ttggtgtaga tgttcgacca ggtgttttcc tgtccgtcga cgacgccggt ctgcagcgcg
      541 ccatacactt cggcaaatgc catcggctgc ggcgaggcgc ccatggcctc aatcatcgcc
      601 ttggcgacgt cggaggtctg cacgcggaac ttcaggccct tggcatcggc tggcgtcagc
      661 agcggcttgt tcgccgagaa gtacttcatg ccgctcatcc agtagccaag gccaacgaag
      721 ccgacgtctt ccatcgagtt caggagcttc ttgccggtgg ggctgttggt gaagcggatt
      781 gcggcgtcca tgtccttgaa gatgaacggc aggtcgaaga cgccgagctt cttggtgtac
      841 gaaccaaact tcgagagcga gggtgcgagc agctggacgt cgccgagcag cagggcctcg
      901 agttccttcg agtcgccgaa tagcgtgctg ttgggataga cctcaacgca gagcgagcca
      961 ttcatttcct tgttgacgcg cgccgcgaag tcgtttgccg caacaacctt cgggtgtgtc
     1021 gtgccgccgg tgacgtgcga gagcttgacg acgcgctcgc ccttgtcgca gtcattggcg
     1081 tgggcaccct gcgcagccag catcagggct gccgtcgaca gagccagtcc gatcaatttt
     1141 ttcattctca tctcctccat gcgttgttgg attcattgac gcgtagcgac cccggtcgga
     1201 accgctgcgt cttcatgcct caatggaaat ggcggagtgg ctcgaatgcg cgccacccgc
     1261 tgccaccgtg cttatatcat gagcgatagc gtggagatca agtcctgacg cggttcggcg
     1321 ccgtatggat gcgccgcaat aatgaggtcg ctgatcatcg aatcgactga ataacgttgt
     1381 ttgcgcacga tcgaaatgcg atatgggatc gtttattcgg cgtctatgtt gcagcgtaaa
     1441 accatctccg tgaggcgctt cgcgcgacac gtcgtccatc gaaacgcgag cgctcgcttt
     1501 tcctcgggat cgaaattgat ctcaccgctc gattcgcggg gacgcagtca tcggtgcagc
     1561 aattggtgag gcccatctga tccttggcgg cgggcctcgt cggcctgcgc ctcgcctggc
     1621 tcggcgcggg atttgcctct gtcattcttg gttttgccaa agccgttttc gggcatggtt
     1681 acaatggctc catgccaatg ccacaagcgg acctcgcgct tgcccgtgcg ggcgaggtcc
     1741 agttttcagc caaaccggtc attgttttcg gggagtttct tgcggattgt tttccggacc
     1801 gcaaggtggc gggtggggcg ccattcaacg tggcgtgtca tctgcagggc ttgggtgcct
     1861 cgccgttgct ggctacacgg attggtcagg acgccggttc gcatttcctg ctcgacgcga
     1921 tgaaacgctg cgcgctcgat gtcgccttgc tgcagttcga tccgctgcgt gcgagcggtc
     1981 aggttgacgt gatcgtcgag ggtggcggtc accgcttcga aatcgcgccg gaccaggcat
     2041 ttgatttcat cgacgcggac ccgcttcttg ctgccgtgga ttgccttgat gcgaagcgcc
     2101 agtccccgtc cctgatctac ttcggcaccc tcgcgcaacg tggcagaggt tcgcgcgagg
     2161 cgcttcgccg cttgctcgat cgatgcgatg caccccgatt cgttgatctg aatctgcggc
     2221 cgccatgggt cagtcgtgac gtctgtggat acgcgctaac acaggcgaag ctggtcaaga
     2281 tgagcgaggc ggaactgcgt acggtggctg gctggcttgg cgaggattgt caaaagagcg
     2341 aaggcgagga tctcggtcgc cgtgtcgcga ctcgctatgg catcgaccgg ctgttcgtga
     2401 cccgcggcga gcaaggcgcg tggtgtgttt ccggcggcga ggtaattcgt gtgtcgtcgc
     2461 ccgcgccacc gtcggcggac ggtgatgccg tcggtgcagg cgatgccttt tccgcagtcc
     2521 tgattgtcgg cacgctcggc ggctggcccc tgtctctgat gctggagcgg gcggcgcact
     2581 ttgcctcggc cgtgtgcgga atacgcggcg cgaccccagc ctcgtcggac ttctaccggc
     2641 catttctcga caactggaat acccgatgac ccgcgcttca cgacaatcgc cgctgcacat
     2701 tatgctgatc aacgttcacg gcctgttgcg cgggcacaac cctgaactcg ggcgcgattc
     2761 cgataccggc ggacagatcg tctacgtgct ggaactggcc aaggcgctgg accgtgaccc
     2821 ggcggtcggc aagatcgatg tattgacccg gcagatcaat gatcccgagg tcgatggtga
     2881 ctatcgggtc gccgaggaat cgctgggcga gaagacaacc ctgctgcgtc tgccctgtgg
     2941 ccccgaaaag tatctgcgca aggagctgct ctggccacac ctcgaccaga tggtcgaccg
     3001 atgtttggtt ctgctccgcg agcgcggccg attacccgac cttatccaca gccactacgc
     3061 cgatgccggt tatgtcggcc ggcagctgtc gctgctgctc ggcattccgc agattcatac
     3121 cggacactcg ctggggcggc ccaagcgcgc gcggctattg gccagcgggc gtaaggccga
     3181 ggccatcgac cgccagtacc atttcgatcg gcggatcgat gccgaagagg acgttctgta
     3241 taacgcgtcg ctggtcatca ccagcacgcg ccaggagatc gacgagcaat acggcctgta
     3301 ccgcaacagt gatcggccgc gatttgccgt gattccgccg gggacagaca ccgagcggtt
     3361 ttccgcgccg gggcgacgca agttgtccga acgtttgctg gcgcagatcg accgcttttt
     3421 gagcgatcac cggaagccga tcatcctgac gatcgcccgt gccgatgcgc gcaagaatct
     3481 cggcggcctg cttgccgcgt tcgccggcga tcgggagttg cgcgagcgcg ccaacctggt
     3541 gatcgttgcc ggcagccggg cgaacatccg cgaaatggat tcggcgcaga gccgagtact
     3601 gcaggatctg ttgctcgaca tcgacctcca cgatctgtgg ggaagcgtcg ccatcccgaa
     3661 ggaaattgcg caggaggaca tcccggatct ctaccgcctc gtcgcgcgac gccatggggt
     3721 gttcgtcaat tcggcgctga ccgaaccgtt cggcctgacg ctgatcgaag cggcagccag
     3781 cggattgccg atcatcgccc ccgacgatgg cgggccgcgc gacatcatcg ccaactgccg
     3841 caacggcgtg ctggtcgata cgctgagtcc ggaggcgatt gccagtgcgc tcaagagcac
     3901 gctcggcgac cagcgtcagc tggaaacctg ggctgccaac gggctggcgg cggtgcagcg
     3961 ccactacagc tgggccgcac acaccaagcg ctacctcaag gaagtgcgcg atgtcatgca
     4021 gcgtgagcgc aagcaggcgc gtcggcggca tcaggtctgg agcggtggaa agtcaacgct
     4081 gccgctggtt cgcgacatcc tgatcagcga tatcgacaac acactgattg gcgacggcga
     4141 tgggttgagc gaactgactg cctggctgca ggcgcggcgc ggccaggttg gcttcggcat
     4201 tgccaccggc aggaccatag acagcacgct gaagattctg aaaaagtggc gcgtcggcgt
     4261 tcccgacgtg ttggttaccg cagtcggcag cgagatctat tacgggccgg gctgcattgc
     4321 cgacacgggt tggtcgtcgc acatccgcca cctgtggcga cgcgacgagc tggccacggc
     4381 gatgacggcg cttcccgggc ttgaacttca ggcggaagcg gatca
//
LOCUS       DATDAA010000377         2572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_61967,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000377 DATDAA010000000
VERSION     DATDAA010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61967"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VIS73_11255"
     CDS             complement(<1..199)
                     /locus_tag="VIS73_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY5763773.1"
                     /translation="MAIYKIGTRQPRLGGGVWIADNATVIGDVRLGEHASVWFNAVLR
                     GDNDPISVGTNANIQDGSVLHT"
     gene            305..2326
                     /locus_tag="VIS73_11260"
     CDS             305..2326
                     /locus_tag="VIS73_11260"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY5763774.1"
                     /translation="MHVFFAPAIALMNRLRFAQKFILMAAIATVLVGVLSWQLVSIRS
                     EQVAVSRIEASGLPIVSAALGLARDIQQHRGLSSGVLGGSLALEPRRAEREKIVGEAI
                     PALEARMPERLRNDSHWASIKARWVEIVAGGLEMSTTLNFAEHSQLVGDMLVIVGDLA
                     DHYRLTNDVDTEIYYLLDAGLVKVPQALEAVGQVRGKGTGVLARKMMFDEESVLLNSR
                     LVEAEGAFRAFDLAVERIGLLDPELGAELAEPAKALKSDFAGFSQLVRSTILLNNFYG
                     VEPKAFFDRTTAVIEKGYEFIDKLALPKAEAGIERRIAALQARTAATLMLIAAMIAAI
                     AYVCIGAYLAINEGVRNLRDAARGMAQGDLTVRAAESSRDEVGEIAHGFNAMAVAVHG
                     LVGEVAQNAEAVLRAAQVLEASAGKIARASGEQSEASSSMAAAVEEMTVGIDHINRNA
                     QEADQNTREAGELSQQGAGAVNGVVHEMNEIAESVQDSARTIRELGEQSKKISAIVGT
                     IREIADQTNLLALNAAIEAARAGEAGRGFAVVADEVRKLAERTAQSTHEIAEIVRAIQ
                     SGTGAAVTAMETGVSRVEKGVVRSREAGESMSGIDRAMQHVVQTVGEISNALREQSAA
                     STELAKAVERIAQQTEQNTAVVADNAKTTAELRQRAEALQEEVGRFRLS"
     gene            complement(2293..>2572)
                     /locus_tag="VIS73_11265"
     CDS             complement(2293..>2572)
                     /locus_tag="VIS73_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763775.1"
                     /translation="GATDARFVAPPELVWPEVMNWFSGLVDWAAGLGKPLGVGLLLLA
                     STLAASGWLAVQVGWRLHLMRAWRARRAARAAREQKSDQDKRKRPTSS"
BASE COUNT          470 a          782 c          882 g          438 t
ORIGIN      
        1 cggtgtgcag gaccgagcca tcctggatat tggcgttggt gcccaccgag atcgggtcat
       61 tgtcgccgcg cagcacggcg ttgaaccaga ccgatgcgtg ctcgcccagc cggacgtccc
      121 cgatgaccgt tgcgttgtcg gcgatccaga cgccaccgcc cagtcgtggt tggcgggtgc
      181 cgatcttgta gattgccatc gcgtagatcc tttctttgtc tggttcctcc ggcattctag
      241 gcgaatcagg ttgccgcgcc ccgctgaatt cttctagact ccggccacta ctcgggggaa
      301 ttccatgcac gtattttttg cgccggccat cgccttgatg aaccggctgc gctttgcgca
      361 gaagttcatc ctgatggcgg ccattgccac ggtcctggtc ggggttctca gctggcaact
      421 ggtcagcatc cgcagcgagc aggttgcggt ctcgcgcatt gaagcgtcgg gcctgccgat
      481 cgtatcggcg gcacttggac tggcgcgcga catacagcag catcgcggcc tgtcgtcggg
      541 cgtactcggc ggcagcttgg ccctcgagcc gcggcgtgcc gagcgcgaga agattgtcgg
      601 cgaggccata ccggcgttgg aagcgcgcat gcccgagcgc ctgcgcaacg attcgcactg
      661 ggcctcgatc aaggctcgtt gggtcgagat cgtcgccggt ggcttggaga tgtcgacgac
      721 gctgaatttt gccgagcatt cgcaactggt cggcgacatg ctggtcatcg tcggcgatct
      781 cgccgaccac tatcggttga ccaacgacgt cgacaccgaa atctactatc tgctcgatgc
      841 cggattggtc aaggtgccac aggcgctcga agctgtgggt caggtgcgcg gcaaggggac
      901 cggtgtgctc gcacgcaaga tgatgttcga cgaagagagc gtcctgctca acagccggct
      961 ggtggaggcc gaaggcgcgt ttcgcgcgtt tgacttggcc gtcgaacgaa tcggcctgct
     1021 cgatccggag cttggtgccg agctggccga accggccaag gctttgaaaa gcgactttgc
     1081 cgggttttca caactcgtcc gcagcaccat cctgctcaac aatttttacg gcgttgagcc
     1141 caaagcgttt ttcgaccgta cgaccgcggt gatcgaaaag ggctacgagt tcatcgacaa
     1201 gctggcgctg ccgaaggccg aggccggaat cgagcgtcga atcgcggcgt tgcaggcacg
     1261 gacggccgcg acgttaatgc tgattgcagc catgatcgcg gcgattgcgt acgtctgcat
     1321 cggtgcctat ctggccatca acgagggcgt tcgcaacctg cgcgatgccg ccagaggcat
     1381 ggctcagggc gacctgacgg tgcgcgccgc cgagtccagc cgcgatgaag tcggtgagat
     1441 cgctcatggt ttcaacgcga tggccgtggc ggtgcacggt ctggtcggcg aggtggcgca
     1501 gaatgccgag gcagtgctgc gcgcggcgca ggtgcttgaa gcgtcggcag gcaagatcgc
     1561 gcgcgccagc ggtgagcaga gcgaggcttc gagttcgatg gcggcagcgg tcgaggaaat
     1621 gaccgtgggt atcgaccaca tcaatcgtaa cgcccaggag gccgaccaga acacccgcga
     1681 ggccggcgag ctgtcgcagc agggggcagg cgccgtcaac ggcgtcgtcc acgagatgaa
     1741 cgagatcgcc gagagcgttc aggattcggc gcggacgatt cgcgagctcg gcgagcagtc
     1801 gaagaagata tccgccatcg tcggcacgat ccgcgaaatc gccgaccaga ccaacctgct
     1861 cgcactcaat gcggcgatcg aggcggcgcg tgccggtgag gcggggcggg gctttgccgt
     1921 cgttgccgac gaagtgcgca agctcgccga aaggaccgcg cagtcgacgc acgagatcgc
     1981 cgagatcgtc cgggcgatcc agagcggcac cggtgcggcg gtgacggcga tggagaccgg
     2041 tgtctcacgc gtggagaagg gcgtcgtgcg ctcgcgcgag gctggcgaga gcatgagcgg
     2101 catcgaccgg gcaatgcagc acgtggtgca gacagtcggt gaaatatcga atgcgctgcg
     2161 cgagcagagc gcagcgagca ccgaactggc caaggccgtc gagcgcatcg cgcagcagac
     2221 cgagcagaac accgcggtgg tcgccgacaa cgcgaagacg accgccgagt tgcgccagcg
     2281 cgccgaggcg cttcaggaag aagtcggacg cttccgtttg tcctgatcgc tcttctgttc
     2341 gcgagcagca cgggcagcgc ggcgcgcgcg ccaggcgcgc atcaggtgta gccgccagcc
     2401 gacctggacc gccagccagc cgctggctgc cagcgttgag gcaagcagca acaggcctac
     2461 acccagcggt tttcccaaac cggctgccca atcgaccagt cccgagaacc agttcatgac
     2521 ctcaggccag accagttcgg gcggtgcgac gaatcgggcg tcggtggcac cg
//
LOCUS       DATDAA010000378         5155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_307795,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000378 DATDAA010000000
VERSION     DATDAA010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307795"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..863
                     /gene="tsaB"
                     /locus_tag="VIS73_11270"
     CDS             141..863
                     /gene="tsaB"
                     /locus_tag="VIS73_11270"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY5763776.1"
                     /translation="MCDKPRRLLAFDASIEPGSAALLVDGSGVDGRAAATGENSSQAL
                     LPAATALLAARKLALGDLDAIAFGCGPGSFTGLRVACGIVQGLAYGLRLPVVPIDSLA
                     AVALLAIDAAPQDSGELLVTMDARMGEVYCAHYRVETGMPRRIGEPQVLPPDEVALPT
                     GVNRCAGNALLAYPLLAERFASAGLTLLPDLLPTAGAVARLAVEALAAGRVVSAMEAT
                     PVYVRDKVALTTRERLAAGGRA"
     gene            869..1330
                     /gene="rimI"
                     /locus_tag="VIS73_11275"
     CDS             869..1330
                     /gene="rimI"
                     /locus_tag="VIS73_11275"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY5763777.1"
                     /translation="MLRSQADFSPLVLTDLDLVVSIEDAIYPFPWTRGNFVDSLRAGY
                     SGWAYRIDGVLIGYAVCMSVLDEIHLLNLSIAQTWQGRGHGARLLRFIMDVYGASGSR
                     QLLLEVRPSNTAAIKLYRQFGFVRIGVRRNYYPAANGHEDAWVMVRELSSC"
     gene            1324..2022
                     /locus_tag="VIS73_11280"
     CDS             1324..2022
                     /locus_tag="VIS73_11280"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY5763778.1"
                     /translation="MLSREQILEEMGLWPIWRLRQSDGSATAAQSVVDSQPATATAPV
                     AGESAWDTLNREIAACRNCGLCAARQQAVVGVGDRQADWLFIGEGPGAEEDQRGEPFV
                     GPAGKLLDAMLAAIGLARGDKVYIANAVKCRPPQNRTPTAEEIATCRPFLERQIDLIK
                     PRLIVLLGKAAVQAVLGREEAMARLRGEVFSFRGVPVLATYHPAYYLRSPLEKARGWE
                     DLCRARRLMREQTD"
     gene            complement(2042..3307)
                     /gene="lplT"
                     /locus_tag="VIS73_11285"
     CDS             complement(2042..3307)
                     /gene="lplT"
                     /locus_tag="VIS73_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEY5763779.1"
                     /translation="MPFGFYIIMAAQFFSALADNALLIAAISLLRDLEAPPEYEPLLK
                     TFFTLSYVLLAAFVGAFADSMPKWRVMFISNLIKIGGCLMMFVGFHPLAAYAVVGLGA
                     AAYSPAKYGILTEYLPHRKLVVANGWIEGLTVGAIILGVVVGGALIRPDVATAIIPPE
                     SFLFTAGLDTAAELTILVVGLFYVIAAIFNMYIPDTGVDHKPLKKNPWYLVHDFTHCF
                     LLLWRDRLGQISLAVTTLFWGAGATLQFIVIKWAEASLNLDLSKSAMLQGVVAIGIAV
                     GAVIAARLITLRKSVRVIPIGIAMGFVVIGMNFIDTYWLAIPMMILIGALSGFFVVPM
                     NALLQHRGHILMGAGHSIAVQNFNENLSILFMTGAYFLMIRADLSIYAVIAMFGLFVS
                     GSVYLVKLRHEANQREYDDVRHLDDGDNH"
     gene            complement(3423..4310)
                     /gene="prmB"
                     /locus_tag="VIS73_11290"
     CDS             complement(3423..4310)
                     /gene="prmB"
                     /locus_tag="VIS73_11290"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY5763780.1"
                     /translation="MTDHLITLRDHLRYSVTRFEAAGLTYGHGSDNAWDEAAYLLCHA
                     LHLPLERLDAFLDARLLPEERAVLLDRIERRASERLPAPYLTGEAWLSGHRFRVDRNV
                     IVPRSFIAELLDEGLNPWIADPMAVGKVLDLCTGSGCLAILAALAFPEASIDAIDISP
                     GALAVAHANRGDYKLESRLRLIESDLFTSLGAERYDLIISNPPYVDADAMAALPDEYR
                     HEPSLALAGGSDGLDLVHRIFAGARARLNPGGLLVVEIGHNRAVLEAAYPDTGFVWLE
                     TEAGDEYVFLLHRDEIPLR"
     gene            complement(4307..>5155)
                     /gene="dapE"
                     /locus_tag="VIS73_11295"
     CDS             complement(4307..>5155)
                     /gene="dapE"
                     /locus_tag="VIS73_11295"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY5763781.1"
                     /translation="RGAADMKGSLAAFVTAAERFVERHPTHPGSIAFLLTSDEEGDAT
                     DGTTVVVEHLRQAGETIDFCIVGEPTAVEVLGDTVKNGRRGSLSGRLTIRGVQGHIAY
                     PHLAKNPIHLAAPAIAELAGISWDSGNAHFPPTSWQVSNIRAGTGATNVIPGTCELLF
                     NFRFSTASTPEGLRSRFESVLHKHGLDFEVQWTLGAQPFLTTNGPLTKAVLAAIDETL
                     GVTATLSTSGGTSDGRFIATICPQLVELGPVNATIHKVDECVAVADLAQLSNLYFRLL
                     ERLLVE"
BASE COUNT          914 a         1562 c         1795 g          884 t
ORIGIN      
        1 acgaggccgt tgccgtctcg actgtttctg atctggcgat gaatcgtctg ggcctcggca
       61 acgccgatct gcggcgcgtt gtcgcgcggc tcgatgctga tctcaaacac accttccaga
      121 tactgtttgg ctgagtcggc atgtgcgata aaccccgccg actgctcgcc ttcgacgcca
      181 gcatcgaacc cggatcggcc gcgttgctgg tcgacggctc gggcgtcgac ggacgcgctg
      241 ccgccactgg cgagaactca tcgcaggcac tgctgccggc ggcaacggcg ctgctcgccg
      301 cgcgcaagct cgcccttggc gatctcgacg cgattgcctt cggttgcggc cccggatcct
      361 tcaccggcct gcgggttgcc tgcggtatcg tccaggggct ggcctacgga ctgcgcctgc
      421 cggtggtacc gatcgactcg ctggcagccg ttgcattgct ggcgatcgat gccgcgccac
      481 aagacagcgg cgagctgctg gtgacgatgg atgcacgcat gggcgaggtc tattgtgccc
      541 actaccgcgt cgagactggc atgccgcgcc gaatcggcga gccgcaggtg ctgccgccgg
      601 acgaggtggc gctgccaacc ggcgtcaacc gttgcgccgg aaacgccttg cttgcctatc
      661 cgctgctcgc cgaacggttc gcgtctgccg ggcttaccct gctgcccgat ctgctgccga
      721 cggccggcgc ggttgcccgt ctggccgttg aggcgcttgc ggcgggacgg gtggtcagcg
      781 cgatggaggc gacgcccgtt tatgtccgcg acaaggtggc gctgaccacc cgtgaacgac
      841 tcgccgcagg ggggcgggcg tgagcgccgt gctccggtcg caagcggatt tttcgccgct
      901 cgtgctgacc gaccttgacc tggttgtgtc gatcgaggat gcgatctacc cgttcccgtg
      961 gacccgcggc aacttcgtcg actcgctgcg cgccggctac agcggctggg cctaccgcat
     1021 cgacggggtg ctgatcggct acgcggtatg catgagcgtg ctcgacgaga ttcacctgct
     1081 caatctcagc atcgcccaga cctggcaggg gcgcggccat ggggcgaggc tgctgcgctt
     1141 tatcatggac gtctatggcg cttcgggttc gcggcaactg ctgctcgagg tccgtccaag
     1201 caatacggcg gcgatcaagt tgtaccggca gttcggtttc gtgcggatcg gcgtgcgtcg
     1261 caattactac ccggctgcca acgggcacga ggacgcctgg gtgatggtca gggagctgtc
     1321 gtcatgttga gtcgcgaaca gattctcgaa gaaatgggcc tgtggccgat ctggcgtctg
     1381 cgccaatccg acgggtcggc gaccgcggcg cagtcagtgg tcgactcgca gccggcaacg
     1441 gccaccgcgc cggttgccgg ggagtccgcc tgggacacac taaaccgtga gatcgctgcc
     1501 tgccgcaact gtgggctctg tgcggcccgc cagcaggccg tggtcggcgt cggcgaccgc
     1561 caggccgact ggcttttcat tggcgaaggt ccgggtgccg aggaggacca gcgtggcgaa
     1621 cccttcgtcg ggcccgccgg gaaactgctc gacgcaatgc tcgcggcaat cggcctagcg
     1681 cgcggcgaca aggtgtatat cgccaacgcg gtgaaatgcc ggccgccgca gaaccgcacc
     1741 ccgaccgccg aggagatcgc cacctgccgg cccttcctcg agcgacagat cgacctgatc
     1801 aagccgcggc tgattgtgct cttgggcaag gccgcagtgc aggcggtact cgggcgcgag
     1861 gaagcgatgg cacgcctgcg cggcgaggtg ttttcctttc gcggcgttcc cgtgctcgcc
     1921 acctaccacc cggcgtatta cctgcggagc ccgctggaaa aggcgcgcgg atgggaggac
     1981 ttgtgtcgag cccggcgact gatgcgcgaa cagacagact gaacccagca atccagctat
     2041 ttcagtggtt gtcgccgtcg tcgaggtggc ggacgtcgtc gtactcgcgc tggttggcct
     2101 cgtggcgcaa cttgaccaga tagacgctgc ccgagacaaa caaaccgaac atcgcgatga
     2161 ccgcgtagat cgacaggtcg gcacggatca tcaggaagta ggcgccggtc atgaagagaa
     2221 tcgacaggtt ctcgttaaag ttctgcacgg caatcgagtg gccggcgccc atcaggatgt
     2281 ggccgcggtg ttgcagcagc gcattcatcg gcacgacgaa gaaccccgac agcgcgccga
     2341 tcaggatcat catcggaatc gccagccagt aggtgtcgat gaaattcatg ccgatgacga
     2401 cgaagcccat cgcgataccg atggggatga cccgtaccga ttttcgcagc gtgatgagcc
     2461 gggcggcgat gaccgccccg acggcgatgc cgatcgccac cacgccctgc agcattgccg
     2521 acttggatag atcgaggttg agcgaagctt cggcccactt gatgacgatg aactgcagcg
     2581 tcgcgccggc accccagaac agcgtggtta ccgcgagcga gatctgcccg aggcgatcgc
     2641 gccagagcag caggaaacag tgcgtgaagt cgtggacgag ataccacggg ttcttcttca
     2701 gcggcttgtg gtcgacgccg gtgtcgggga tgtacatgtt aaagatcgcc gcgatcacgt
     2761 agaacaggcc gacgacgaga atcgtcagct cggccgccgt atcgaggccg gcggtaaaga
     2821 ggaagctctc gggcggaatg atcgcggttg cgacgtcggg gcggatcagc gcgccaccga
     2881 cgacgacgcc gagaatgatc gcgccgacgg tcaggccctc gatccagccg ttggcgacga
     2941 cgagtttgcg gtgcggcagg tactcggtga ggatgccgta cttggccggg gaataggcgg
     3001 cggcgccgag gccgacgacg gcgtaggcgg cgagcggatg gaagccgacg aacatcatca
     3061 ggcagccgcc gatcttgatc aggttgctga tgaacatgac acgccacttc ggcatcgagt
     3121 cggcgaaggc gccgacaaat gccgcgagca gcacgtagga aagcgtgaag aaggtcttca
     3181 gcagcggctc gtactcgggc ggcgcctcga ggtcgcggag cagcgagatg gcggcaatca
     3241 gcagcgcgtt gtcggcgagc gcagaaaaaa actgcgccgc catgatgatg tagaagccga
     3301 aaggcatcga ctgctgaggg gtccgcgagg ggttgtcgaa ctcgttgcga ggctggccgg
     3361 aagggcttcg cgacggggta gcagtcttat aacacggatg ctttaccgca ggctttcatg
     3421 gatcagcgaa gcggaatttc gtcacggtgg agcaggaata cgtactcgtc gccggcctcg
     3481 gtttcgagcc aaacgaatcc ggtgtccgga tacgccgcct cgagcaccgc gcggttgtga
     3541 ccgatctcga ccaccagcag gccccccgga ttcaggcgcg ccctggcgcc ggcaaatatc
     3601 cggtggacca ggtcgagtcc gtcgctgccg ccagccagcg ccaggctcgg ttcgtgtcgg
     3661 tattcatcgg gcagtgccgc catcgcatcg gcatcgacat agggcggatt ggagatgatc
     3721 aggtcgtagc gctcggcgcc tagcgaggtg aacaggtccg actcgatcag ccgcagacgg
     3781 gattcgagtt tgtagtcacc gcggttggca tgggccaccg cgagcgcgcc gggggagatg
     3841 tcgatggcgt cgatactggc ttcgggaaag gccagtgccg caagaatcgc caggcaaccc
     3901 gatccagtgc acaggtcgag cactttgccg accgccatcg gatcggcgat ccaggggttc
     3961 aagccttcgt cgagcagttc ggcaataaac gagcggggga cgatgacgtt gcggtcaacg
     4021 cggaagcggt gacccgagag ccaggcttcg ccggtcaggt agggcgccgg caggcgttcg
     4081 ctggcacgtc gttcgattcg gtcgagcagc acggcacgtt cttcgggcag caaccgggcg
     4141 tcgaggaagg catcgaggcg ttcgagcggt aggtgcagcg cgtggcagag caggtaggcg
     4201 gcctcgtccc aggcgttgtc gctgccgtgg ccgtaggtga gcccagctgc ctcgaagcgc
     4261 gtgacggaat agcgcaggtg gtcgcgaagg gtgatcaggt ggtcggtcat tcgacgagca
     4321 gtcgttcgag caggcggaag tagaggttgg acagctgcgc gaggtcggca accgcgacgc
     4381 actcgtcgac cttgtggatc gttgcgttga ccggaccgag ctcgaccagt tgcgggcaga
     4441 tcgtcgcgat gaagcggccg tcggaggtgc caccggatgt cgacagcgtc gccgtgacac
     4501 cgagggtctc gtcgatggcg gcgagcaccg ccttggtcag gggtccgttg gtggtcagga
     4561 aaggttgggc gcccagcgtc cactgcacct cgaagtctag gccgtgcttg tgcagcaccg
     4621 actcgaagcg actgcgcagc ccctcgggcg tgctggctgt cgagaagcgg aaattgaaga
     4681 gcaactcgca ggtgccggga atcacattgg tcgcgccggt gccggcgcgg atattggaga
     4741 cctgccaact ggtcggtgga aaatgcgcgt tgccggaatc ccaggagatc ccagccagtt
     4801 cggcgattgc cggtgcggcc agatgaatcg ggttcttcgc cagatgcgga taggcgatat
     4861 gcccctggac tcccctgatc gtcaaccggc ccgacagcga gccgcggcgg ccgttcttga
     4921 ccgtgtcgcc aagcacctcg acggcggtcg gctcgccgac gatacagaag tcgatcgtct
     4981 cgccggcctg cctcaggtgt tcgacaacga cggtggtgcc gtcagtggca tcgccttcct
     5041 cgtcggaggt cagcaggaag gcaatgctgc ccgggtgggt cgggtgccgt tcgacgaatc
     5101 gttcggcggc cgtgacgaag gccgcgagcg agcctttcat gtcggcagcg ccacg
//
LOCUS       DATDAA010000379         2089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_62026,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000379 DATDAA010000000
VERSION     DATDAA010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62026"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VIS73_11300"
     CDS             <1..1059
                     /locus_tag="VIS73_11300"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY5763782.1"
                     /translation="DTVGYTVPTEFDRLFQYLKEHVPGIDGITLSVHCHDDLGMAVAN
                     SLAAVAAGARQVECTINGIGERAGNCSLEEIVMALKTRSEFFGLDTAIDTRRLYPTAR
                     LVSGITGMAIPRNKAIVGENAFAHEAGIHQHGMLQHHSTYEIMRPDDVGISKSNLVLG
                     KHSGRHAFRDRVRELGFALDEFETNRAFQEFKKLADRKKDIYDGDIEAIIMNVDSASS
                     GPWILRSLEISTATDEQAAASLRLMDEKGKERNETAKAEGPVEAAFRVLEETTGIDLD
                     LKKFELHSASVGEDAQGEATVTVEYDGYSYRGHGTSTDIVEAACRAYLEVINRILRRR
                     ELGKQDGPVAADINRASI"
     gene            1074..2033
                     /locus_tag="VIS73_11305"
     CDS             1074..2033
                     /locus_tag="VIS73_11305"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEY5763783.1"
                     /translation="MSFEGGKLIWLNRRMVPWEQATVHVLSHALHYGSSVFEGIRMYK
                     TPDGPKMFRLTDHMQRLFDSAKIYRLPIPYERDELIAACKEVVLANELSNGAYIRPIA
                     LRGYGDIGLAPKPEHPVDVAIAAWEWGAYLGKEGLEQGVDVCVSSWQRVAPNTVPALA
                     KAGGNYLSSTLISTEAKRLGFAEGIALSTDGTVSEGAGENLFVIREGKIATPSAASSI
                     LTGITRDTVMKLAARAGLTVEEKSIPRESLYLADEMFFTGTAAEITPIKSVDRIQIGE
                     GRRGPITKQLQDAFFGLFSGETEDEEGWLESLDAPPVAAAGGT"
BASE COUNT          486 a          600 c          640 g          363 t
ORIGIN      
        1 gacaccgtgg gttacaccgt gcccacggaa tttgatcgcc tgttccagta tctcaaggaa
       61 catgtgcccg gcatcgacgg cataacgctg agcgttcatt gccacgacga tctgggtatg
      121 gcggttgcaa acagtctcgc cgcggtcgct gcgggcgcga ggcaggttga atgcacaatc
      181 aatggcattg gggaacgggc aggcaactgt tcgctcgagg aaatcgtaat ggcgctcaag
      241 acgcgcagcg aattctttgg cctcgatacg gcgatcgata ccaggcggct ctacccgacg
      301 gcgcgactcg tctccggcat taccggcatg gccattccac gaaacaaggc gatcgttggc
      361 gagaacgcat tcgcacatga agccggcatt catcagcatg gcatgctgca gcatcattca
      421 acctacgaga tcatgcggcc cgacgacgtc ggcatcagca aatccaacct ggtgctcggc
      481 aagcacagcg gacgccacgc gtttcgcgac cgggtgcggg aactgggatt cgcgctcgat
      541 gaattcgaga ccaaccgtgc cttccaggaa ttcaaaaaac tggccgaccg caagaaagac
      601 atttacgacg gcgatatcga agccatcatc atgaacgtgg acagtgcctc ttccggaccg
      661 tggatactga gatctctgga gatttccacg gccacggatg aacaggcggc cgcaagcctc
      721 aggctgatgg acgaaaaggg taaggagagg aacgaaaccg caaaggcgga agggccggtc
      781 gaggcagcgt ttcgggtgct cgaggagacg accggcatcg acctggattt gaagaaattc
      841 gaattgcaca gcgcgtcggt cggtgaggat gcccagggtg aggcgaccgt caccgtggaa
      901 tatgacggct acagttatcg tggacacggc accagcaccg atatcgtcga ggcagcctgc
      961 cgtgcatacc tcgaggtcat taatcgcata ctgcgacgtc gtgaactcgg caagcaagac
     1021 ggaccggtgg ccgcggacat caatcgcgca tctatttaat gtaagctacg cgcatgagct
     1081 ttgaaggtgg aaaattaatc tggctgaatc ggcgcatggt gccgtgggag caggcgacgg
     1141 ttcacgttct gtcgcatgca ttgcattatg gatcctccgt gttcgaaggc atccgcatgt
     1201 acaagacgcc cgacggaccc aaaatgttcc ggctgacgga ccacatgcag cgcctcttcg
     1261 attcggcaaa aatctatcgg ctaccgatac cctatgagcg cgacgaactg atcgccgcct
     1321 gcaaggaagt cgtgctcgcc aacgagctga gcaacggcgc ctacatacgc ccgatcgcct
     1381 tgcgtggcta tggcgacatc ggcctggcgc cgaagccgga gcatccggtc gacgttgcaa
     1441 tcgcggcctg ggaatggggc gcctacctcg gcaaggaagg actcgagcaa ggcgtcgacg
     1501 tctgtgtgtc gagctggcag cgggtcgcgc cgaacacggt ccctgccctg gccaaagccg
     1561 gcggcaatta cctctccagc acgctgatca gcaccgaggc aaaacgtctc ggctttgccg
     1621 agggaatcgc gctgtccacc gacggcaccg tcagcgaagg cgccggcgaa aacctgttcg
     1681 tcattcgtga aggcaagata gccaccccgt cggcggcctc gtcgatactc accggcatta
     1741 cacgcgatac cgtcatgaaa ctcgcagcgc gtgcgggact gaccgtggaa gaaaagtcga
     1801 taccgcgcga gtcgctgtac ctcgccgacg agatgttttt cacgggaacc gcagcggaga
     1861 tcacgccgat caagtctgtg gaccggatcc agattggcga aggccggcga ggtccgatca
     1921 cgaagcaact gcaggacgcg ttcttcggcc tgttttccgg ggaaaccgag gacgaggaag
     1981 gatggctcga aagtctcgac gcaccaccgg tcgccgcggc gggcggcacg taacaaaaaa
     2041 gtgggcgcaa aaaacgcaag gacgcttttc gagaaaatct ggtccgagc
//
LOCUS       DATDAA010000380         9781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_516878,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000380 DATDAA010000000
VERSION     DATDAA010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9781
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516878"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VIS73_11310"
     CDS             complement(<1..513)
                     /locus_tag="VIS73_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5763784.1"
                     /translation="MYLQHFGLQELPFSLTPDTGFTFAHAGHQSALNTVMLALQNGEG
                     FVKVTGEVGTGKTLLCRRLLRALRDDYATAYLPNPNLDPAALYRALGEELGVAEVGGG
                     DAYQLHKAFNAALLKIARDGRSVVLLVDEAQAMPAETLEALRLLSNLETEKRKLLQIV
                     LLGQPELDERL"
     gene            complement(524..2239)
                     /gene="mshL"
                     /locus_tag="VIS73_11315"
     CDS             complement(524..2239)
                     /gene="mshL"
                     /locus_tag="VIS73_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HEY5763785.1"
                     /translation="MTASHRLPSRILLACLIGLGACSSTFRHGDDTLTRIGDEMKAPP
                     APATSEQRVAAQLLPPLEVPLPKMADAIEPRFDLAVNNAPAAEVFLAIASGTRYSMLL
                     PNDLSGSVTLSLKDVTVHEALDAMRELYGYQYRVSGTRVYVQPNTLQSRVFQVNYLSG
                     RRQGMSELRVTSSSISSSQNPGSPISGSASASSTPAQPATGSGSRGSSESSRVVTSSD
                     SDFWAELRISLEALVGNADGRSVVINPMSGIVVVRGMPDDLRNVERYLAATQLTVERQ
                     VMLEAKIIEVVLNDSFQSGINWAALGGANRRYAIGVSGANATLDTSGVFGTNSVGVLP
                     GAGGALTTSAGGTGFYGLALQSNNFAGLLNFLETQGSVQVLSSPRIATLNNQKAVLKV
                     GADEFFVTNVTTTTSSTTSGSTTSPTITLQPFFSGIALDVTPQIDDQNNIILHVHPAI
                     SVVAEKQKQIDLGSLGLFNLPLAASAINETDSIVRVQDGTVVAIGGLMKQEQTDSRSQ
                     VPGAGDVPGLGALFGQRAKAFRKSELVVLIKPTIIQGEQSWARDLLDTRERLQKYGVP
                     ADSSR"
     gene            complement(2236..2679)
                     /locus_tag="VIS73_11320"
     CDS             complement(2236..2679)
                     /locus_tag="VIS73_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763786.1"
                     /translation="MKAWRTLLLTLAIGAIAGAAAAETLRDPTRPAFDIDAGKGVASA
                     RPGLPAGDPPATSYVAPRGPRLQSILTRSDGRSLAVIDGKTLAVGDRFGSRRLIAIDI
                     ASVTLRGPEGRRVLHLTPAATKTNSRTELRKQQSPAGAQPGRNYP"
     gene            complement(2676..3359)
                     /locus_tag="VIS73_11325"
     CDS             complement(2676..3359)
                     /locus_tag="VIS73_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763787.1"
                     /translation="MKAQWDQLALRFAAISQRERLMLAGATLLAIGFVLNLLWLSPAL
                     GQRATAHKVLAQKSAEAQTLRAQIAQLKQQADLERVQGEATLKSLQQDYRVVSDALGE
                     LGRNLIAAREMPAFLRNVMPRTRGVEIVAVRTLSVAPLLPVAENANDGGAQAAADAAD
                     PGNLYRHSVEIKLAGRYSDLYDWLLALERAPKKVLWGDLTLEVQKYPVNVLTLTVHTL
                     SLDRAWLSI"
     gene            complement(3356..3991)
                     /locus_tag="VIS73_11330"
     CDS             complement(3356..3991)
                     /locus_tag="VIS73_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763788.1"
                     /translation="MPGYINLYDPALRRPREWVTLNSLALAAGVLLVLMLALGAGLRW
                     DAARADGQRQALEADMAALSGEMAAVSAPAGALAEMRRKIAETRAALTRKSEVIEVLQ
                     RGGMLRSDAFSGYLAGLSRRDVDGLWLTGFSIGPGPLAMEIRGRVSTSSALPAYLDQL
                     GSDTAFAGRSFASLAVARGSSSEGDGRPYYADFVLKAELPADPSASRGGGK"
     gene            complement(3991..4908)
                     /gene="pilM"
                     /locus_tag="VIS73_11335"
     CDS             complement(3991..4908)
                     /gene="pilM"
                     /locus_tag="VIS73_11335"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY5763789.1"
                     /translation="MGKAANTGGLVGICPHGDGALVLGLSSATDAPMVRLVDVLPLDR
                     ADPEALRKAIRDKGLRSARFALLLGASDYQLLQADAPAVEEGELREALRWSIKDSVSF
                     SVEQASIDFIELPTRGSAAGRSRQLLVAAASHTSLRPWVEFFQDAGLDLAVIDVPELA
                     LRNLAVRFESGKRGFLFLNFDDEGGLLTFVHDGELLNWRRLETTLPMIAEITPDRREG
                     LFDRIALELQRSVDNFERQSGGINIGRLALLQSDGLDGLIAHLEGNLSLPVEAVELSS
                     VIGFDAKLPAGVQPAHLATLCGLALRAET"
     gene            complement(4996..7623)
                     /locus_tag="VIS73_11340"
     CDS             complement(4996..7623)
                     /locus_tag="VIS73_11340"
                     /inference="COORDINATES: protein motif:HMM:NF042738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HEY5763790.1"
                     /translation="MTNSRKSLLRASIQWVCAALFLAAGAAHGACSSYAGQISINEYN
                     LISSNTPTGLPYVELKVLNPGVVTATSNFAGWTVRSYTGVQANKIFDRNLQSIYTSAN
                     TSANNVCGPTTSEYIRIPFGNNELQEDANLVLSDGAGNVVDVFRVSRTASLPDFYSGD
                     YSVCARANLPFDTDLANVDPNRKNIQRLPDGTGNWAQSPSNGNNSEDSLCTSNDNILL
                     VTKITGSPNPAVDTDVAYAISIRNGGNAAMTNVTATEVLPAGLNFVSATGLSQGSFSS
                     ATGVWAVGSLAAGAAATMTLTARTTAAGQFVNTVTGRAAELGDGFTQGSATINVTSGA
                     APGSFNACHDSTACPPASRLFTRIAGVPFALNLAALKGDGSVETTFSQSAAVSLVSGS
                     CPAGTVVANLGTSTFASGRATVVGISVPSAQRQLRVRIDHQGSSYCSGDAFAIRPSTL
                     TLTSNPLLNNASLTGLPKAVAGSFFTLAASSGVPSGYDGIPAIAGSVVGHDSVIVAPG
                     TLVGAFGVADGISSSGVFSYHDVGRVIFAADAIRDTGYTVVDQAAGDCLPGSAANTPD
                     ASGRVGCWIGSAQSGLGRFIPARFNAQLSVAAACGGASGFTYLGQDALAVNLQVTAES
                     ESGTVLSRYNAALPTMATLTLGALNGGAAFDSTRISQPALPAPGFTAGAFIFSDTVAV
                     DRLQAGPDGPFDDFRFSLSVSDPQDAVVFEKRNGVDLSPFATTVESDPTRLRYGRLRM
                     SNAYGSELTQLPVPLLAESWTGSAWAQNTLDACTALSTPLPGASFSEYQRNLNVGETS
                     GTLANPLAAGNARLILSAPGAGNAGAVRITIASPVWLKYRWDDVDQGGDGDIFDDDPS
                     ALISFGVSRNNFIFMRENY"
     gene            complement(7632..8138)
                     /locus_tag="VIS73_11345"
     CDS             complement(7632..8138)
                     /locus_tag="VIS73_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763791.1"
                     /translation="MRRESKRAASRHHGFALPSAIFLLVVLAGLAGFIVTLNSTQQRA
                     QVQDVEGVRAFWAARGAVDYALTLALAPADTSGATSFAPCPAGVPGGTVNGFSIVLSC
                     ERSPAAGHHTESGVNLVIYTVQATASRGTLGGLGYVERQVSVIAAKCKDPNASLPGGS
                     PDPRHRCL"
     gene            complement(8128..9105)
                     /locus_tag="VIS73_11350"
     CDS             complement(8128..9105)
                     /locus_tag="VIS73_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY5763792.1"
                     /translation="MRCRSMSSSAALASPSRSADCVSALPPMCPTDPMAGTGFSRGFT
                     LIEMIMTIAITGIVAGMVVVFLRQPIDAFVDTGRRAEMSDTADGAIRRMVRDVRRAVP
                     NTLRVLRVGSIDYVEFIPARAGARYRFDNACFTSGCSSLTTMGSLVDDASGLPRICTG
                     VGTPAGCPAADRIIVFNPYNNAAGDCSVANPSAYCEFVGGDPAATVTGVTPAGDADVV
                     QFVNSRFRPGNGSAAARFQIAEQPVTYACDPISGTITRFSGYGVQAVQPTPPLGGQSH
                     LLARGISACAMTYASGAFERWALLAMQFTLTQAGESATFYQEVHVDNAP"
     gene            complement(9011..9598)
                     /locus_tag="VIS73_11355"
     CDS             complement(9011..9598)
                     /locus_tag="VIS73_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY5763793.1"
                     /translation="MCTSRRVRLSQFRRQRGMSLVELIIFIVIVGVSVAGVLAVFSTT
                     ARSSADPLVRKQLIAVAEALLEEVALQPFTYCDPDDGNALWANAAATGAGDCATQAEA
                     IGPEAGESRYSLLTPFDNVNDYAGFSMSPVRDLQNLPIPALAPYSAAVAVSPVGGSFG
                     LAADDALQIDVVVSGAGESFTLSGLRFRFAPNVSH"
     gene            complement(9588..>9781)
                     /locus_tag="VIS73_11360"
     CDS             complement(9588..>9781)
                     /locus_tag="VIS73_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763794.1"
                     /translation="SGPDGSPFAVSAPAGTVIAPVLVFRFDCLGQPMQNDTQPLATTT
                     FTVGGGRTVTVEAETGYVH"
BASE COUNT         1783 a         3147 c         3214 g         1637 t
ORIGIN      
        1 caggcgctcg tcgagttcgg gctggccgag cagaacgatc tgcagcagtt tccgtttttc
       61 ggtttcgaga ttggacaaga gccgcagtgc ctcaagcgtc tcggccggca tggcctgcgc
      121 ctcgtcgacg agcaggacga ccgagcggcc gtcgcgggcg atcttgagca gggcggcgtt
      181 gaaggccttg tgcagctgat aggcatcgcc gccgccgacc tcggcgacgc caagctcctc
      241 gccgagcgca cgatagagcg ccgcggggtc gaggttggga ttgggcaggt aggccgtcgc
      301 gtagtcgtcg cgcagcgcgc gcagcagccg gcgacagagc agggtcttgc cggtgcccac
      361 ctcgccggtg accttgacaa agccttcgcc gttctgcagc gccagcatga ccgtgttgag
      421 cgccgactga tggccggcgt gggcaaaggt gaagccggtg tcgggcgtca gcgagaaagg
      481 cagttcctgc agtccgaagt gctggaggta caaggccgtc ccgtcagcgt gacgagtccg
      541 cgggaacgcc gtacttctgc aggcgctcgc gggtatcgag caggtcgcgc gcccagctct
      601 gttcgccctg gatgatggtc ggcttgatca gcacgaccag ttcgctcttg cggaaggcct
      661 tcgcacgctg cccgaacagc gcaccgaggc cgggcacgtc accggcgccg ggcacctggc
      721 tgcgcgaatc ggtctgctcc tgcttcatca ggccgccgat ggcgaccacc gtgccgtcct
      781 ggacgcggac aatgctgtcg gtctcgttga tcgccgacgc ggcgagcggc aggttgaaca
      841 ggccgagcga accgagatcg atctgcttct gcttctcggc gacgacgctg attgccggat
      901 gcacgtgcag gatgatgttg ttctggtcgt cgatctgcgg cgtgacgtcg agcgcgatac
      961 ccgagaagaa cggctgcagc gtgatcgtcg gcgaggtcgt cgatcctgag gtggtcgagg
     1021 aggtggtggt ggtgacgttg gtgacgaaga actcgtcggc accgaccttg agcacggcct
     1081 tctggttgtt gagcgtggca atgcgcgggc tcgacagcac ctgcacgctg ccctgcgttt
     1141 cgagaaagtt gagcaggccc gcgaagttat tgctctgcag ggccaggcca tagaagccgg
     1201 tgccgccggc cgatgtcgtc agcgcaccac cggcaccggg cagcacaccg accgaattgg
     1261 tgccgaacac gccgctggtg tcgagcgtgg cgttggcgcc cgacacacca atcgcgtagc
     1321 gccggttggc gccgccgagc gcagcccagt tgatgcctga ctggaaggaa tcgttgagga
     1381 cgacctcaat gatcttggct tcaagcatca cctgccgctc aacggtcagc tgcgtcgccg
     1441 cgaggtaacg ttcgacgttg cgcaggtcgt cgggcatgcc gcgaacaacg acgatgcccg
     1501 acattgggtt gatgacaacg ctgcggccat cggcattgcc gaccagcgcc tcgagcgaga
     1561 tgcgcagctc tgcccagaaa tcgctgtcgg aggaagtgac gacgcggctc gattccgagg
     1621 aaccgcgcga accgctgccg gtcgccggtt gtgccggcgt gctgctcgcg ctggccgagc
     1681 cgctgatcgg cgaaccggga ttctgcgacg acgagatcga actgctggtg acgcgcagct
     1741 cgctcatccc ctgccggcgg cccgacaggt agttgacctg aaaaacgcgc gactgcagcg
     1801 tgttgggctg gacatagaca cgcgtgccac tgacccggta ttggtagcca tagagttcgc
     1861 gcatggcgtc gagcgcctcg tggacggtga cgtccttgag gctcagagtc acgctgccgg
     1921 agagatcgtt gggcagcagc atgctgtagc gcgtacccga tgcgatggcc agaaagacct
     1981 cggcggccgg cgcgttgttg accgcgagat caaagcgcgg ctcgatcgca tcggccatct
     2041 tcggaagcgg cacctcaagc ggcggcagca gttgcgcggc gacgcgctgt tcgctggttg
     2101 ccggcgccgg cggcgccttc atctcgtcgc cgattcgcgt cagcgtgtcg tcgccgtggc
     2161 ggaacgtgct gctgcaggca ccaaggccaa tcaggcaggc gagcaggatg cggctcggga
     2221 gtctatggct tgcggtcacg ggtaattcct tcctggctgt gccccggcgg gcgactgctg
     2281 cttgcggagt tcggtccgac tgttcgtctt ggtggcggcc ggcgtcaggt gcaggacccg
     2341 acggccttca gggccgcgca gcgtcacgct ggcgatgtcg atggcgatca ggcgccggct
     2401 gccgaagcga tccccgacgg ccagggtctt gccgtcaatg accgccagcg aacggccatc
     2461 gctgcgggtc aggatgcttt gcagacgcgg gccgcgcggc gcgacgtagc tcgttgccgg
     2521 cgggtcgccg gccggcaggc ccgggcgcgc gctggcaacg cccttgccgg cgtcgatgtc
     2581 gaacgctggc cgggtgggat cgcgcagggt ctcggctgcc gcggcgccgg cgatcgcgcc
     2641 gatagccagc gtgaggagga gcgtgcgcca ggccttcata tcgacagcca tgcgcggtcg
     2701 aggctcagcg tgtgcacggt cagcgtcagc acgttcaccg ggtacttctg cacctcaagc
     2761 gtcagatcgc cccagaggac tttcttcggc gcccgttcga gcgccagcaa ccagtcgtag
     2821 agatcggaat aacgaccggc gagcttgatc tcgacgctgt gccgatagag gttaccgggg
     2881 tcggcagcgt ctgccgccgc ctgggcgccg ccatcgttgg cgttttcggc caccggcagc
     2941 aagggcgcga ccgacagcgt gcggaccgca acgatttcga caccgcgcgt gcgcggcatc
     3001 acgttgcgca ggaaggccgg catttcgcgg gcggcgatca ggttgcgtcc cagttcgccg
     3061 agcgcatcgc tgacgactcg gtaatcctgc tgcaggctct tcagcgtggc ctcgccctgc
     3121 acacgctcga gatcggcctg ttgcttcaac tgggcgattt gtgcgcgcag tgtctgtgct
     3181 tcggcgctct tctgcgccag caccttgtgc gcggtggcgc gctgccccaa ggcaggcgac
     3241 agccagagca ggttgagaac gaagccaatc gcaaggagcg ttgcgccggc gagcatcagc
     3301 cgctcgcgct gcgagatcgc cgcaaagcgc agcgccagct gatcccactg cgccttcatt
     3361 tcccaccccc gcgcgacgcg ctcggatcgg ccggcaattc ggccttcagc acgaaatcgg
     3421 cgtagtacgg ccggccatcg ccttcgctgc tgctgccgcg tgctaccgcc aggctggcga
     3481 aactgcgacc ggcgaaggcc gtatcgctgc cgagctggtc gaggtaggca ggcagcgcgg
     3541 aagacgtgga cacgcgaccg cggatctcca tcgccagcgg gccggggccg atcgaaaagc
     3601 cggtcagcca tagaccgtcg acgtcgcgcc gggagaggcc ggccagatag ccggaaaacg
     3661 cgtcggaacg cagcatgccg ccgcgctgca ggacctcgat gacttcgctt ttgcgggtca
     3721 gcgccgcgcg ggtctcggct atcttccggc gcatctcggc cagcgcgccg gccggcgcgc
     3781 tgactgcggc catctcgccg ctgagcgcgg ccatgtctgc ttccagcgcc tggcgctggc
     3841 cgtcggcgcg cgccgcatcc cagcgcaacc cggcgccaag ggcaagcatc agcaccagca
     3901 gaacgccggc ggcgagtgcc agcgaattga gcgtcaccca ctcgcgcggg cggcgcagtg
     3961 ccggatcgta gaggttgatg taaccgggca tcaggtttct gcccgcagcg cgaggccgca
     4021 gagcgtcgcc aggtgggccg gctgcacgcc ggccggcagc ttggcgtcga aaccgatcac
     4081 cgaagacagc tcgaccgcct cgaccggcag cgagaggttg ccctctaggt gcgcgatcag
     4141 gccgtcgagc ccatcgctct ggagcaaggc gagacgaccg atgttgatgc cgcccgactg
     4201 gcgctcgaag ttgtcgaccg agcgctgcag ttccagcgcg attcggtcga acaagccctc
     4261 gcggcggtcg ggggtgatct ccgcgatcat cggcagcgtc gtctcgaggc gccgccagtt
     4321 gagcagttct ccgtcatgaa cgaaggtgag caggccgcct tcgtcgtcga agttgagaaa
     4381 gaggaaaccg cgcttgccgc tctcgaaacg aacggcgaga ttacgcaacg ctagttccgg
     4441 cacgtcgatg accgcgaggt cgagcccggc gtcctggaag aactcgaccc agggacggag
     4501 actcgtgtga ctggccgctg cgaccagaag ctgccgagac cggccggcgg cgctaccgcg
     4561 ggtcggcaac tcgatgaaat cgatcgacgc ctgctcaacc gaaaagctga ccgaatcctt
     4621 gatcgaccat cgcagcgcct cgcgcaattc gccttcctcg accgccggcg catccgcctg
     4681 cagcaactga tagtcactgg cgccgagcaa caacgcgaaa cgagcactgc gcagtccctt
     4741 gtcgcggatc gccttgcgca gggcttcggg gtcggcgcga tccaatggca gcacatcgac
     4801 taggcgcacc atcggcgcat cggttgcaga cgagagaccg agcaccaagg cgccgtcgcc
     4861 gtgcggacaa atgccgacca accctccggt attcgctgcc ttacccattg acgtttccaa
     4921 agctgcgggc gacgccccga aaacctatgc gccgcctgcg aatggcggcg atgataaccg
     4981 ctgacacccc tgtcatcagt agttttcccg catgaagatg aaattgttcc gtgacacgcc
     5041 gaagctgatc aacgcgctcg ggtcatcatc aaatatgtcg ccgtcgccac cttgatcgac
     5101 atcgtcccag cggtacttga gccaaacggg cgacgcgatc gtgatgcgca cagccccagc
     5161 gtttcccgct ccaggggccg aaagtatcag ccgcgcgttg ccggcagcca gcggattcgc
     5221 cagcgtgccg ctggtctctc cgacattcag attgcgctga tattccgaaa aacttgcccc
     5281 cggcagcggc gtcgagagcg cagtacaggc atcgagcgtg ttttgtgccc acgcgctacc
     5341 ggtccaagac tcggcaagta gcggcaccgg cagctgtgtt agctccgagc cgtaggcatt
     5401 gctcatccgc agtcgtccat aacgaagcct agtcgggtcg gactcaaccg tcgtcgcaaa
     5461 gggcgacagg tcgacgccgt tgcgcttttc gaagacaacg gcatcctgtg ggtcactgac
     5521 gctcaggctg aagcggaagt cgtcgaacgg cccgtcagga ccggcctgca agcgatcgac
     5581 ggcaaccgtg tcggagaaaa tgaatgcgcc ggccgtaaaa cccggcgccg gcaatgctgg
     5641 ctggctaatg cgcgtcgaat cgaaggccgc gccgccgttg agcgctccga gcgtcagcgt
     5701 cgccatcgta ggcaaggccg cgttgtagcg gctaagcacg gtgccgcttt cgctttcggc
     5761 ggtaacctgc aggttaaccg ccagcgcatc ctgaccgaga taggtgaaac cgctggcgcc
     5821 accgcaggca gcggcgaccg acaactgggc attgaaacgc gccggtatga atcgcccaag
     5881 cccactctgt gcgctgccga tccagcaacc cacacgacct gacgcgtcgg gagtgttggc
     5941 cgcgctaccg gggagacagt ctcccgccgc ctggtcgacg accgtataac cggtatcgcg
     6001 tatcgcgtcg gccgcaaata tcactcgccc gacatcgtga tacgagaaga ccccgctgga
     6061 cgaaataccg tcggcgacgc caaaagcacc gaccagcgtg cccggcgcga caatgaccga
     6121 atcgtggccg accacgcttc cggcaatggc tggtataccg tcgtaaccgc ttggcacgcc
     6181 cgaacttgcc gctagcgtga aaaacgaccc ggcgaccgcc tttggcaggc cagtcagtga
     6241 agcgttgttc agcagcgggt tgctcgtgag cgtcagcgtc gaggggcgaa tcgcaaaggc
     6301 gtcaccactg cagtaactgg aaccttggtg gtcgattcgt acacggagtt ggcgctgggc
     6361 gctaggcacg gaaatgccga cgactgtcgc ccgcccactg gcgaaggttg acgtcccgag
     6421 gttggccacc acggttccgg ctgggcagct tccggatacc agacttacgg cggcgctctg
     6481 tgagaatgtc gtttcgacgg aaccatctcc tttcagcgcc gccagattca gcgcgaaggg
     6541 tacgccggca atacgggtaa acaagcgact tgcgggaggg cacgccgtcg aatcgtgaca
     6601 ggcattgaag ctacccggtg ccgcgccgga agtcacattg attgttgcag aaccttgggt
     6661 aaaaccgtcg ccaagttctg ccgctcgacc ggttaccgtg ttgacaaatt gcccggccgc
     6721 ggtggtccgt gcggttaagg tcatcgtcgc ggctgcccca gctgccagcg acccaacggc
     6781 ccagaccccg gtcgcgcttg agaaacttcc ttggcttagt ccagtcgcag acacgaaatt
     6841 cagtccggct ggcaacactt ctgttgcggt gacgttggtc atggcggcat tgccgccgtt
     6901 gcgaatcgaa atagcgtatg caacgtcggt atcaaccgcc ggattcggac tgcccgtgat
     6961 cttggtgacc agcaaaatgt tgtcattgct ggtgcacaac gagtcctcac tgttgtttcc
     7021 gttagacggg gactgagccc aattgccggt gccgtccggt aaccgctgga tattcttacg
     7081 gttggggtcg acgttggcca ggtcggtatc gaaaggcagg ttggcgcgcg cgcagaccga
     7141 atagtcgcct gagtagaagt ccggcaacga ggcggttcgc gacaccctga acacgtcgac
     7201 aacattcccc gcgccgtcgc ttaacactag gttcgcgtcc tcctgtagct cgttgttgcc
     7261 aaacgggata cgaatgtatt ccgaagtcgt cggtccgcag acgttgttcg ccgatgtatt
     7321 ggcgctcgtg tagatcgatt gcaggtttcg gtcaaatatc ttgttggcct gcacccctgt
     7381 atacgagcga accgtccaac ccgcgaagtt ggacgtggcc gtgaccacac ccgggttcag
     7441 caccttcagt tccacgtacg gtaaacccgt gggagtgtta gaagaaatca ggttgtactc
     7501 attgatactg atttgcccgg cgtagctcga acaggcgccg tgcgccgcgc cggcggcaag
     7561 aaacagcgcg gcacaaaccc actgaataga tgcacgcagt agcgacttac gcgagttcgt
     7621 cattaagagc ctcacaagca tcggtgacga gggtccggag aaccaccggg cagcgaagca
     7681 ttcgggtctt tgcactttgc tgcgatcacg ctgacctgcc gctcgacgta acccaacccg
     7741 cccaaggtgc cgcgactggc ggttgcctgg accgtgtaaa tcacgaggtt aaccccggat
     7801 tcggtatggt gcccagccgc cggcgagcgc tcgcaactga gcacaatcga aaacccgttg
     7861 accgtgccgc cgggaacacc ggccgggcat ggcgcaaagc tcgtcgcgcc actcgtgtcg
     7921 gccggcgcca gcgcaagggt cagcgcatag tcgacggcgc cgcgcgccgc ccagaaggcg
     7981 cggacgcctt cgacatcctg tacctgcgcc cgctgctgcg tcgagttcag tgtgacgatg
     8041 aatcccgcga gacccgccag caccaccagc aggaatatcg ccgatggcag cgcgaacccg
     8101 tgatgacggg acgcggcgcg cttcgattca cggcgcattg tcaacgtgaa cctcctgata
     8161 gaacgtcgcc gattcgcccg cctgggtcag cgtgaattgc atcgccagca gcgcccagcg
     8221 ctcgaaggcc ccggacgcat aggtcatcgc gcaggcgctg atgccgcgcg ccaacagatg
     8281 cgactgcccg ccgagcggtg gtgttggctg caccgcctgc acgccgtagc cgctgaatcg
     8341 cgtaatcgtg ccggaaatcg gatcgcaggc gtaggtcacc ggctgctcgg cgatctggaa
     8401 gcgcgctgcg gccgaaccgt tgcccggccg gaaccggctg ttgacgaact gcacgacgtc
     8461 ggcgtctccg gccggggtga cgccggtaac cgttgctgcg gggtcaccgc caacgaattc
     8521 gcagtaggcg ctgggattgg ccaccgaaca gtcgccggcg gcattgttat agggattaaa
     8581 gacgatgatg cggtcggcag ccggacaacc ggccggcgtc ccgactccgg tgcatatccg
     8641 cggcagtccg ctcgcgtcat cgacgagact gcccatcgtc gtcaggctgc tgcagccgct
     8701 cgtgaagcac gcgttgtcga agcgataccg cgcgccagcc cgggcgggaa tgaactcgac
     8761 gtaatcgata ctgccgacgc gcagcacacg caaggtgttc gggactgccc ggcgcacgtc
     8821 gcgcaccatg cgccggatcg ccccgtctgc cgtatccgac atctctgccc gccgcccggt
     8881 atcgacgaag gcgtcgatcg gctgacggag gaagacgacc accatgccgg cgacgatgcc
     8941 ggtaatggcg atcgtcatga tcatctcgat caacgtgaag cctctcgaga aaccggttcc
     9001 ggccatggga tcagtgggac acattggggg caaagcggaa acgcaatccg ctgagcgtga
     9061 aggactcgcc agcgccgctg acgacgacat cgatctgcag cgcatcgtcg gccgccagcc
     9121 cgaacgaacc gccaaccgga ctcaccgcca ccgcagcact gtacggagcc agcgccggaa
     9181 tcggcaggtt ctgcaggtca cggaccggac tcatcgaaaa cccggcgtag tcgttgacgt
     9241 tgtcgaacgg tgtaagcagc gaatagcgag actcccccgc ctcggggccg atcgcctcag
     9301 cctgcgtcgc gcagtcgccg gcgcctgtcg ctgccgcatt cgcccagagg gcattgccgt
     9361 cgtcggggtc gcagtaggtg aagggctgca acgcgacttc ttcgagcagc gcctcggcaa
     9421 cggcgatcag ctgcttgcgc acgagcggat cggcgctgct gcgcgccgtc gtgctgaaaa
     9481 ccgccagcac gccggcgacc gaaacgccga cgatgacgat gaagatgatc agctcgacca
     9541 gcgacatgcc tcgctggcgc cggaattggc tcagccgaac gcggcggcta gtgcacatag
     9601 ccggtctctg cctcgacggt gacggttcgc ccgccgccga cggtgaaagt cgtggtcgcc
     9661 agcggctgcg tgtcgttctg catcggctgg ccgaggcaat cgaaacggaa cacgagcacc
     9721 ggtgcaatga cggtgccagc cggcgccgac accgcgaagg gcgaaccgtc gggaccgctc
     9781 a
//
LOCUS       DATDAA010000381         3098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_516959,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000381 DATDAA010000000
VERSION     DATDAA010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516959"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VIS73_11365"
     CDS             <1..1073
                     /locus_tag="VIS73_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763795.1"
                     /translation="EPVATPAVEIPAVALAAPAVAAVMPSTRDAAPLARSRVAAIKLE
                     KGKARSDRLIIELRGDEPDMVDLRLEDDQLKVSFTGVRLGENIAPKKDNWPGSRVVAS
                     VEAVDLGGSVDLTIHSRVAAWSYRGAQGKRRLVIDLTAAAKQPVAKAAKPAVVAKAQR
                     PKPAAKPSPKAAAPAKPVVAKAPAKAAASTAASTATAAAGTAATSAAAAVASVAAAAP
                     APAAKVAEPPVQIALAMPESRPAAKPAKKERGKNLKYKDGSDLAPCPPARPEDQIIGA
                     FDEDAVKEILDRIGCPQCHAYVQKKTGPPFREVIKKYKGDPACVIHRLKTNDTHNEEG
                     VTDDIAASEFKPIADYVATRIK"
     gene            1235..2146
                     /locus_tag="VIS73_11370"
     CDS             1235..2146
                     /locus_tag="VIS73_11370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HEY5763796.1"
                     /translation="MPETKTVRNAATRLALALAVLLPAGILAPAAQAQEERELKPCVE
                     CHEAFFAIADSKHFVAGDSRTPKGQGAECESCHGDALAHNKAPRSKDRLPTRFGAKQP
                     AEPQNAKCLTCHQTGTRIHWQGSQHDRAQVPCASCHKAHAAKDPVMVEETQAGVCFEC
                     HKDRRADMLKVSAHPLKTGFFVCSSCHQSHGSTTRQLLAKNTVNDTCYTCHADKRGPF
                     LWEHRPAVDDCTNCHNPHGTNNPPMLKVRLPYLCQNCHQAGGHPPVAMSGTEIASGDI
                     RMIGRACVNCHQKVHGSNHPSGARLHR"
     gene            2189..>3098
                     /locus_tag="VIS73_11375"
     CDS             2189..>3098
                     /locus_tag="VIS73_11375"
                     /inference="COORDINATES: protein motif:HMM:NF023282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HEY5763797.1"
                     /translation="MTQNQIRFRPRADSVATVAALAALAALSSPAAGEPMLHAPSLYR
                     ASDMSRFVDNYVELGIGYNSKDSYKFGEWNGLREKGAYPIFGFNWLSRARDNGANYWE
                     LRGSGLGLETRKFGLEGGSQGRWRLNASADRLVRSELDSARFVHTVGLGTNNLSGISP
                     PTTVPYKIEQGRDFYRLGFSATLSDNWNASVNYREDRRDGTRVMGAPFEGGLGRPLNV
                     PYQIDDHTQQVDAQLSYADELSQLQFAYHFSRYENDAGALELENDSATVVGRVSLMPS
                     NDYHQLSLTGAYRVTASTRVAAKLSYG"
BASE COUNT          651 a         1036 c          909 g          502 t
ORIGIN      
        1 cggagccagt cgctacgccg gccgtggaga ttccggccgt tgctctcgcc gccccggccg
       61 tcgccgccgt catgccgtcg acccgcgatg ctgcaccgct ggcacgctcg agggttgccg
      121 ccatcaagct tgaaaagggc aaggcacgca gcgaccgcct gatcatcgaa ctgcgcggcg
      181 acgaacccga catggtcgac ctgcgtctgg aagacgatca gctgaaggtc agcttcaccg
      241 gcgttcgcct cggcgagaac attgcgccca agaaggacaa ctggcccggt agccgcgtcg
      301 tcgcctcggt cgaggcggtc gatctcggtg gcagcgtgga tctgacgatc cactcgcgcg
      361 tcgccgcctg gagctatcgc ggcgcgcagg gcaagcgccg tctggtcatc gacctcaccg
      421 ccgccgccaa gcagccggtg gccaaggcag cgaagcctgc cgtcgttgcc aaggcccagc
      481 gaccgaagcc cgcagccaag ccgtcgccga aggcggccgc gcctgccaag cccgtcgttg
      541 caaaggcccc ggcgaaagcg gctgcttcaa cggccgccag cacggctacg gccgcggcag
      601 gaacggccgc aacatcggct gccgccgccg tcgcgagcgt tgctgccgcc gcaccagcgc
      661 cggccgcgaa ggtcgccgaa ccgccggtgc agatcgcgct ggccatgccg gaatcccgcc
      721 cggccgccaa accggcgaag aaggagcggg gcaagaacct caagtacaag gacggctcgg
      781 acctcgcccc ctgcccgccc gcaagacccg aagaccagat catcggcgcc ttcgacgagg
      841 acgcggtcaa ggaaatcctc gaccgcatcg gctgcccgca atgtcatgcc tacgtccaga
      901 agaagacggg cccgcccttc cgcgaggtca tcaagaagta caagggtgat cccgcctgtg
      961 tgatccaccg cctcaagacc aacgacacgc acaatgaaga aggcgtgacc gacgacatag
     1021 ccgcctccga gttcaagccg attgccgact atgtcgcgac gcggatcaag taatccgccg
     1081 ccgcgccgta aatcgggata ggcttgcttc gccagaaaca atttacaatc aaatcaatta
     1141 gaagcaccat tgatttaaga ttttttgtta ggatttagcc gcagcctcaa ggggcttgag
     1201 gcgcaaaacc cgaaacaggg gatgggtaat gccgatgccg gaaacaaaga ccgttcgtaa
     1261 cgccgccact cgactggccc ttgccctcgc cgtgctcctg ccggccggga tcttggcgcc
     1321 ggcagcacag gcacaagaag agcgcgagct caagccctgc gtcgagtgcc atgaggcttt
     1381 ttttgccatc gccgacagca agcacttcgt cgccggcgat tcgcgtacgc ccaaaggcca
     1441 gggcgccgaa tgcgaaagtt gccacggtga cgcgcttgcc cacaacaagg cgccgcgttc
     1501 gaaggatcgt ctgccgaccc gcttcggagc caagcaaccc gctgaaccgc aaaacgcaaa
     1561 atgcctaacc tgccaccaga ccggcacccg tatccactgg caaggtagcc agcacgatcg
     1621 ggcccaggtt ccctgtgcca gttgccacaa agcgcacgcg gccaaggatc cggtgatggt
     1681 cgaggagacc caggctggcg tctgcttcga gtgccacaag gaccgtcgtg ccgacatgct
     1741 caaggtttcg gcgcacccgc tgaagaccgg gttcttcgtc tgttcttcgt gccaccagag
     1801 ccacgggtcg accacgcgcc aactacttgc taaaaacacc gtcaacgaca cctgctatac
     1861 ctgccacgcc gacaagcgcg gtcccttcct ctgggaacac cggccggctg tggatgactg
     1921 cacgaactgc cacaacccgc acggcaccaa caatccgccg atgctgaaag tgcggttgcc
     1981 ctacctgtgc caaaactgtc accaagcggg cggccatccg ccggtcgcaa tgagcggaac
     2041 ggagattgcg tccggagaca taaggatgat cggccgcgcc tgcgtcaact gtcaccaaaa
     2101 ggtacacggc tccaaccacc cgtctggtgc gcgcctgcat cgctgagccc cgatcacgaa
     2161 ccgaatcatg aagtccagag gtgaggacat gacccagaac cagattagat tccgtccccg
     2221 cgccgattcc gtggcaactg tcgctgcgct ggctgcgctg gctgcgctgt cgtcgcctgc
     2281 ggccggcgag ccgatgctgc acgcgcccag cctgtaccgc gcttccgaca tgtcgcgctt
     2341 cgtcgacaac tacgtcgagc tgggcatcgg ctacaacagc aaggactcgt acaagttcgg
     2401 cgaatggaac ggcctgcgtg aaaagggcgc ctatccgatc ttcggcttca actggttgtc
     2461 gcgcgcccgc gacaacgggg ccaactactg ggagctccga ggctctggac taggtctcga
     2521 aacccggaaa ttcggcctcg aaggtggctc acaggggcgc tggcgactga acgcgagcgc
     2581 cgatcgtctg gttcgctctg agttagacag cgcgcgcttc gtacacacag ttggtttggg
     2641 caccaacaac ctttccggca tatcaccgcc taccacagtc ccctacaaga ttgagcaggg
     2701 gcgtgacttc tatcggctgg gatttagcgc cacactgtca gacaactgga atgcatcggt
     2761 taactaccgt gaagaccgcc gcgacggcac tcgggttatg ggtgcgccat ttgaaggagg
     2821 tctgggtaga ccgctgaatg tgccctacca gattgatgac cacacccagc aagtagacgc
     2881 gcagctgtcc tatgcagatg agctttcaca acttcaattc gcttatcatt tttcacgcta
     2941 cgaaaacgac gctggcgcgc ttgaactgga gaatgactcc gcaactgttg tgggacgcgt
     3001 ctccctcatg ccgagcaatg actaccatca gctatcgctg acgggcgctt accgggtgac
     3061 cgcgagcacc cgggtagccg ccaaactttc ctacggcg
//
LOCUS       DATDAA010000382        16284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_447485,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000382 DATDAA010000000
VERSION     DATDAA010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 16284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16284
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447485"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VIS73_11380"
     CDS             <1..448
                     /locus_tag="VIS73_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HEY5763798.1"
                     /translation="ARRAARSGADTGIASGREADVLVRLAAGEAIGSLLSTQATPLAA
                     RKQWLADHLQLAGALWLDAGAEKALLQGRSLLPIGVTRVEGDFERGEALACRSPDGRE
                     VARGLVNYSSAECRKIAGKASTAIESLIGYVDETELIHRNNLVLTG"
     gene            complement(438..749)
                     /locus_tag="VIS73_11385"
     CDS             complement(438..749)
                     /locus_tag="VIS73_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763799.1"
                     /translation="MKHANHPFLPLFSTFSHFKARLTHGQARFVIFSMLTTTYRHFLR
                     SNLENKFIKNQWAIPETKCVALRVAKQAQAIVADSDVAAGEAGREAAGCLAPPSGRIS
                     R"
     gene            741..1193
                     /gene="mraZ"
                     /locus_tag="VIS73_11390"
     CDS             741..1193
                     /gene="mraZ"
                     /locus_tag="VIS73_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY5763800.1"
                     /translation="MFQGATALSLDTKGRISVPARYRDDLLAAGQGRLVLTAHPHGCL
                     LLFPLPAWEPLRDQILKTPSFNAQSQPLKRVLVGNAREESLDAAGRLLIAPELRDTAQ
                     LEKTVWLVGLGTHFEIWSDAGWKRQNSLAHDAMQSVASAPPGFEDIAL"
     gene            1190..2131
                     /gene="rsmH"
                     /locus_tag="VIS73_11395"
     CDS             1190..2131
                     /gene="rsmH"
                     /locus_tag="VIS73_11395"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY5763801.1"
                     /translation="MSDAAGHRTVLLNEAIDGLAPRAEGIYVDATFGRGGHSRELLKR
                     LGPRGRLIAIDRDPDAVKSGRAISDSRFALHHAAFSELAAVLVQAGLEGAAGVVDGVL
                     LDLGVSSPQIDVAARGFSFRQDGPLDMRMDTTRGETAADWLARADEKDIAEVIKKNGE
                     ERFAVQIAKKVVAARSGERIARTRQLAALVAQAVRSREPGQDPATRTFQALRIHVNRE
                     LEELSLILPLAFDALRGGGRLVVISFHSLEDRMVKRFMRDRATPLAPPKGVPVRAAEI
                     PPAPARLVGRAIRASMAEIAENPRSRSAVLRVLEKLH"
     gene            2136..2402
                     /gene="ftsL"
                     /locus_tag="VIS73_11400"
     CDS             2136..2402
                     /gene="ftsL"
                     /locus_tag="VIS73_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEY5763802.1"
                     /translation="MARLNFLLTLILTVLALVAVTAQHRARSAFQALESAEQHARALE
                     EEYSQLQLEMSTWAQHGRIERIASERLRMHLPAPEAMIHAGGGL"
     gene            2399..4198
                     /locus_tag="VIS73_11405"
     CDS             2399..4198
                     /locus_tag="VIS73_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY5763803.1"
                     /translation="MSGSRAHKYAESPVLKLSMPPWRSRFLLVLLVSGMLLLIARAFQ
                     LQVLEAEFLNREGESRYLREMSIEASRGRIFDREGNVLAVSTPVRSIWAVPSDVNATP
                     AQIQEIAKLLGQTPAELKRKLASDKRFVFLARQIAPETADRVSAMKIAGVFQDKEFRR
                     FYPGAELSAHLVGFTGVDGKGLEGIELALQADLTGVDGARTVIRNRRGEIVEDVGLRK
                     PARDGRDVALALDSKIQYIAYTQLRQAVTEHRAKAGSVVVLDARNGEVLALANWPTYN
                     PNNRQGLSGSALRNRAVTDTFEPGSTLKPFTVALALEKGRYRFNSRIDTTPGRLTIGR
                     ATISDAKPHGVLTVAEVLQKSSNVGTAMMAADLPSRDMWTMYDALGFGKPPALGFPGE
                     VGGRLRAWNGWRPIEKATMSYGHGISMSLVQLARSYTVFGNGGRLLPLSLLKRDYQAS
                     IGTPLLAPQAAREVLAMLEMAVQDGGTAPKARIPGYRVAGKTGTAHKLEGGSYANKYV
                     ASFVGLAPVSSPRLIVALMIDEPSAGKYYGGDVAAPVFAGIMEASLRSLGVMPDLTRL
                     QATARSTPPPATPEAPAREAQVAAANLTVERQL"
     gene            4195..5691
                     /locus_tag="VIS73_11410"
     CDS             4195..5691
                     /locus_tag="VIS73_11410"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY5763804.1"
                     /translation="MIGVVDSIAEEAIARLAAQGVAVRRIACDSRRVGPGDVFMAWPG
                     ARHDARNYIIDAVGRGASGVIWEARAYRFPAALRVANVGVEDLQRHAGEIAHLVYGKP
                     SEAMRLIGITGTNGKTSCSHWLADALAASGERCAVVGTLGNGLPGRLAGSANTTPDAI
                     TLHALLAEWQRDSIAACAMEVSSIGLDQHRLSGARFDTAVFTNLTHDHLDYHGDMDAY
                     AAAKARLFDWPDLRAAVLNLDDPFGVDLAERSGAPIKIGYTLTGKSAPGLSAIVAARD
                     VHATPTGTAFTLACGEDSQRVVVPLIGRHNVQNLLAVAGALLVGDYGIERIAALLPLL
                     KAPPGRLQTLGGSGVPLVVIDYAHTPDALRNVLEALRPVAESRGGRLIAVFGCGGNRD
                     KGKRPQMGEIAARLADRVWLTSDNPRHEEPLAIIADISAGVLAAGVKPTVDADRAHAV
                     RTAIAAADARDVVLLAGKGHEAYQEIAGERLPYSDAITAQRSLARELP"
     gene            5688..7124
                     /gene="murF"
                     /locus_tag="VIS73_11415"
     CDS             5688..7124
                     /gene="murF"
                     /locus_tag="VIS73_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY5763805.1"
                     /translation="MSGAFDMTLAEVAQAIAAERIGGKPQTLATGVVTDSRAEVAGAL
                     FVALKGEHFDGHEYLAAVKAAGAVAAIVDRRRVAELSVIGLPLIAVADPRRTLGDLAA
                     VWRKRFAIPLVGVTGSNGKTTTKEMIAAIFAARARRTGLADAAVVATRGNLNNEIGVP
                     MMLLELTPQTRYAVIEMGMNHPGEIARLAALAAPTVAVVTNAQRAHLEGMGSLAAIVA
                     EKASIFEALSADGVAIINADDPNAERFARVAGARRQLLASLDGSGDVVGRYRPTAAGS
                     TVEISDVSGTVEIALPLFGRHNARNAVLATAAALAAGATLSDAAAGLATLRNVGGRLR
                     LRAAKGGARLLDDSYNANPDSMRAAIDVLADSPGTRILVIGDMGEIGPERLALHAEMG
                     AYARASGIERLFALGESARAAVEAFGAAGTHWKTPEDLSAALLPLLDADTTVLVKGSR
                     FMRMERVADALADESSATTQTKEDARCS"
     gene            7115..8203
                     /gene="mraY"
                     /locus_tag="VIS73_11420"
     CDS             7115..8203
                     /gene="mraY"
                     /locus_tag="VIS73_11420"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005297383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY5763806.1"
                     /translation="MLLMIAQWLAEDVRAFNVFTYITLRAVLAAMTALVIAFIAGPRV
                     IRWLAEKKIGQAVRSDGPQTHLIKSGTPTMGGVLILIAILLTTLLWGDLTNRYIWVVV
                     FVTAGFGAVGWIDDWRKVVQRNPRGLPARWKFFWQSMIGVAAALYLGLWPTVPAAQEL
                     IVPFFKTIAYPLGAFGFIVLTYLVIVGTSNAVNLTDGLDGLAIMPTAMIASALAIFAY
                     VAGHVVFARYLGLPHIPGAGELAVFCGAMAGAALGFLWFNTYPAEVFMGDVGALALGA
                     ALGTVAVIVRQEIVLAIMGGVFVAETLSVAAQVLYFKATGGKRIFRMAPLHHHFELGG
                     WKETQVVVRFWIITIMLVLFGLSTLKLR"
     gene            8200..9579
                     /gene="murD"
                     /locus_tag="VIS73_11425"
     CDS             8200..9579
                     /gene="murD"
                     /locus_tag="VIS73_11425"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY5763807.1"
                     /translation="MRLAGKNILVLGLGESGLAMARWCARQGSALRVADSRAAPPNAA
                     ALARELPAAQLDCGPFTEALLDGIDRIALSPGVALAEPIVQAALRRGIPVVSEIELFA
                     EGLRGQSEAAQILAITGSNGKTTTTALAAALSTAAGRVTVAAGNISPSALDALMAALD
                     AGALPELWVLELSSFQLETTATLGPVAAAVLNLSEDHLDRYVDMAAYAAAKARILDGA
                     ATAVLNREDPRVLAMRRDGQKTVTFGLDVPPTPEDFGLVDGWIVRGGTRLVAQNLLRL
                     HGSHNAANAMAALALCEAAGIELSTVLPALCAFAGLPHRVEWIARIDGVDYYDDSKGT
                     NVGATLAALQGMGRPVAIILGGDGKGQDFSPLVEAIATHARHVALIGRDAARLEAVLA
                     GCGVPMTRCVDMPSAVAACASAAQAGDAVLLSPACASLDMYRSYAHRAEVFVAAVRSL
                     PTYRAEAER"
     gene            9576..10739
                     /gene="ftsW"
                     /locus_tag="VIS73_11430"
     CDS             9576..10739
                     /gene="ftsW"
                     /locus_tag="VIS73_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY5763808.1"
                     /translation="MIGADLAHTHREPAEIDMAIVWSAFALLLIGAVMVYSASIAIAQ
                     GSAFAGYKPEFFLIRHVVFVGTGLLAALVAFQIPLNLWQKMAPVLFLAAIVLLAIVLI
                     PGIGKSVNGAQRWLPLGIINLQPSELMKLFVVLYAADYTVRKLPLMHDLKRAFLPMAA
                     VLVVVGGLLLAEPDFGAFVVIIGIAMGVLFLGGMKTRLFALLTVILLVAFVLLIWSSA
                     YRRERILGFMDPWADPLGRGYQLSHALIAFGRGEVFGVGLGASVEKLFYLPEAHTDFL
                     LAVIAEETGFLGVALVVVLFAVLVQRAFAVGRRAISLERPYAGLVAQGLGLWLGAQAF
                     INMGVNMGLLPTKGLTLPLMSFGGSGIVANCMAVAVLLRVDWENRQLMRGGGW"
     gene            10864..11916
                     /gene="murG"
                     /locus_tag="VIS73_11435"
     CDS             10864..11916
                     /gene="murG"
                     /locus_tag="VIS73_11435"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY5763809.1"
                     /translation="MIMAGGTGGHIMPGLAVADLVRARGWSVVWLGNPEGMEARLVPA
                     KGYPLAPVKFSALRGKGVLRKLLLPLALLRGFYDSLRAIRRWRPDVVIGFGGYVSFPG
                     GMMASLLGRPLAIHEQNAIAGLTNRVLARLADAVLCGFPKALPGGQWCGNPVRAAIAE
                     LPAPELRYGERSGPLNVLVVGGSLGAVALNDVVPRAVARFAASERPRVCHQAGERHID
                     TLRAGYAEAGVAAECIPFIDDMAAAYAEADLVICRAGALTVAEVAAAGVAAIFIPFPH
                     AVDDHQTVNARFLADAQAAILLPQSQLDADRLATTLRSLDRDALMAMAIKARALARPD
                     ATETVAATCERLGGKA"
     gene            11913..13304
                     /gene="murC"
                     /locus_tag="VIS73_11440"
     CDS             11913..13304
                     /gene="murC"
                     /locus_tag="VIS73_11440"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY5763810.1"
                     /translation="MKHKIKHLHFIGVGGSGMSGIAEVLANQGYRVSGSDLSASATTE
                     RLAKAGVRIAIGHDAANITGADAVVVSTAVKADNPEVLAAREAHLPVVPRALMLAELM
                     RIKQGIAIAGTHGKTTTTSLVASILAEAGFDPTFVIGGRLNAAGVNARLGQGEFLVAE
                     ADESDASFLHLSPVISVVTNIDADHMETYGHDFSRLKQTFVEFLNRLPFYGVAVLCVD
                     DANIREIMPEVAKQIVGYGFAAEASIRAEDLVADGAQMRFTLVRQNGVTTRTPVVLNT
                     PGRHNVLNALAAIAVATEIGADDAAILKALAEFKGVGRRFQRCGEIAAIGGGHYTLVD
                     DYGHHPVEMEATLAAARGAFPGRRIVLAFQPHRYTRTRDCFEDFVRVLSNADAVVLSE
                     VYAAGEAPIVAADGRSLARALRVAGRVEPVFAETIDEMENAIGELARDGDVVLVMGAG
                     SIGGLPSRLGACS"
     gene            13301..14215
                     /gene="murB"
                     /locus_tag="VIS73_11445"
     CDS             13301..14215
                     /gene="murB"
                     /locus_tag="VIS73_11445"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY5763811.1"
                     /translation="MSEPLNFAQLRGRWQADVPMGPQLSWRAGGHARRAYVPADIDDL
                     CACIATLPADEPLLFVGLGSNLLVRDGGFPGTVFLLHGTLRQLQLDGEFIYAEAGVTS
                     PKLARFVARQGRVGGEFLAGIPGTIGGALAMNAGCYGGETWPLVDRVLVVDRRGERRV
                     MAAAEFSFAYRHVEPRDKAEAPLFFLAAWFRFPQGDGEASRQRIAELLAKRQATQPLD
                     LPNAGSVFRNPEGDFAARLIEAAGLKGLTVGGAQVSTKHANFIVNFAGCASAADIEAL
                     IETVQNEVQVRFGVSLQREVKIVGEPKR"
     gene            14212..15141
                     /locus_tag="VIS73_11450"
     CDS             14212..15141
                     /locus_tag="VIS73_11450"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY5763812.1"
                     /translation="MSGSDFGKVAVLLGGDSAEREVSLNSGGRVLAALTRGGVDAHAF
                     DPASRPLAELAEFDRVFIVLHGRHGEDGTIQGLLELMGIPYTGSGVLASAVGMDKWRC
                     KLLWQAAGLSLPDYRLLDANSDFDAIEAALGLPLFVKPACEGSSLGVTMVTRPGMLRS
                     AYEEAAVFDPLVIAEKAVLGGEYTVGILGSGESMQALPIIKIEPATAFYDYEAKYTRD
                     DTRYLCPAGLSPEREAELRAESLRAFRILGGRGWGRVDFLMDGDGKAYFLEANTAPGM
                     TDHSLVPMAARAAGIDYDELVLRVLALAQTGGG"
     gene            15145..15885
                     /locus_tag="VIS73_11455"
     CDS             15145..15885
                     /locus_tag="VIS73_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEY5763813.1"
                     /translation="MWHRPNLLTATADLLFFAGAFVLAIVATLALARAPWFPLREVVV
                     SEPLRETRRPEIEAALRDSLHPSGIWTVSTERVRTAIERLPWVRRAEVRRVWPHRLVI
                     HIEEHRAVARWGAGNAQLLNDRGEVFFASTSRPPRLELIGPLGSAAEVLARHREFGNL
                     LAPLGAPIARLELSSRLAWRIRLDDGLLIELGREQARAPVAARLKRFAAAYRELIADA
                     SRRAAVVDLRYVNGFAMRLAGNGAGAVN"
     gene            15893..>16284
                     /locus_tag="VIS73_11460"
     CDS             15893..>16284
                     /locus_tag="VIS73_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY5763814.1"
                     /translation="MGKESRNLIVGLDIGTSKIVALVAELDDNGAINVLGVGTQDSKG
                     LRKGVVVNIEETVGAISRAIQEVELMANRQVAEVYTGIAGSHIKSFNSSGTVAIKDKE
                     VTPMDVERVIEVARAMPIPAEQQILHILT"
BASE COUNT         2557 a         5417 c         5464 g         2846 t
ORIGIN      
        1 ggcgcggcgc gctgcgcgca gcggcgccga tacggggatc gcctccggcc gcgaggccga
       61 cgtgctggtt cgcctggctg ccggcgaagc catcggcagc ctgctgagca cgcaggcgac
      121 gccgctggcg gcgcgcaagc agtggctcgc cgaccacctg cagctggccg gggcgctgtg
      181 gctcgacgcc ggtgccgaga aggcgctgct tcaggggcgt agcctgctgc cgatcggcgt
      241 cacgcgcgtc gaaggcgact tcgagcgcgg cgaggcgctg gcttgccgca gccccgatgg
      301 tcgcgaagtg gcgcgtggtc tggtgaacta cagttccgcc gagtgccgca agatcgccgg
      361 caaggcgtcg acggcgatcg aatcgctgat cggctacgtc gacgaaaccg aactgatcca
      421 ccgcaacaac ctcgtcctca ccggctgatc cggccgctcg gcggcgcaag gcagccggct
      481 gcttccctcc ccgcttcccc cgcagcgacg tctgagtccg cgacgatcgc ttgcgcctgt
      541 ttggcgactc ttaaagcgac acatttagtt tccggtattg cccattgatt tttaatgaat
      601 ttgttttcca ggtttgatcg caggaagtgt cgataagttg ttgtcagcat tgaaaaaatc
      661 acgaatcgtg cttgaccgtg ggtaagccgt gccttaaagt gggagaaggt ggaaaaaagt
      721 ggtagaaagg ggtggttggc gtgtttcaag gggcgacagc gctcagtctc gataccaagg
      781 ggcgaatctc cgtcccggcc cgctatcgag acgatttgct tgctgccggc caaggccggc
      841 tggttctgac cgcccatccg cacggctgcc tcctgctgtt tccgctcccc gcctgggagc
      901 cgctgcgtga ccagatcctc aagaccccta gcttcaatgc ccagtcgcag ccgcttaagc
      961 gcgtgttggt cggcaatgcg cgcgaggaat cgctcgacgc cgccggccgc ctgctgatcg
     1021 ctcccgaact gcgtgacacc gcgcagctgg aaaaaactgt ctggctggtc ggcttgggaa
     1081 cccatttcga gatatggagc gacgctggct ggaagcgtca gaacagtctc gcgcacgacg
     1141 ccatgcaatc ggtggcgagt gctccccccg gattcgagga cattgcgctg tgagcgatgc
     1201 cgctgggcat cgcaccgtac tgctgaacga agcgatcgac ggactggcgc cgcgcgccga
     1261 gggaatctac gtcgatgcca ccttcggtcg cggtgggcac agccgggaat tgctcaaacg
     1321 gctcgggccg cgcggccgcc tgatcgcgat cgatcgtgat cccgatgcgg tcaagtccgg
     1381 ccgggcaatc agcgactcac gtttcgcact gcatcacgcg gccttttcgg aactggcggc
     1441 ggtgctggtg caggcaggtt tggagggtgc tgcgggggtg gttgacgggg tgctgctcga
     1501 cctcggcgtg tcctcaccgc agatcgatgt ggcagcacgg gggttcagtt tcaggcagga
     1561 cggcccgctc gacatgcgca tggatacaac gcgcggcgag acggcggcgg actggctggc
     1621 gcgggccgat gaaaaagaca ttgcggaggt gatcaaaaaa aatggcgaag aacggtttgc
     1681 tgtccagatt gcaaagaagg ttgtggctgc tcggagcggc gaacgtattg cccggacaag
     1741 gcagctggca gcgctcgtcg ctcaagccgt gcgcagccgg gagcctgggc aggacccggc
     1801 gacgcggacc tttcaagctc tacggattca cgtcaaccgg gagcttgaag agctatctct
     1861 gatcctgccg ctggcgttcg atgccctgcg cggcggcgga cgcctggtgg tgatcagctt
     1921 tcactcgctg gaggaccgca tggtcaagcg tttcatgcgt gatcgggcaa cgccgctggc
     1981 accaccaaag ggcgtgccgg tgcgtgccgc ggaaatcccg ccggcgccag cgcgtctggt
     2041 tggtcgtgcg atccgcgcgt cgatggccga gattgccgaa aacccgcgat cgcgcagcgc
     2101 tgtcctgcgc gtcctcgaga agctgcacta gcgacatggc ccgcctgaac ttcctgttga
     2161 cgctgatcct cacggtcctc gcgctcgttg ccgtgaccgc gcagcatcgc gcgcgttcgg
     2221 cgtttcaggc gctggaaagc gccgagcagc acgctcgcgc gctcgaggag gagtacagcc
     2281 agctgcagct cgagatgtcg acctgggcgc agcacggccg catcgagcgc atcgccagcg
     2341 agcgtctgcg catgcacctg ccggcgcccg aggcgatgat tcatgcggga ggcggcctgt
     2401 gagcggttcg cgcgcccaca agtacgccga gtcgccggtg ctaaagctgt cgatgccgcc
     2461 atggcgctcg cggttcctgc tcgtgctgct ggtatccggc atgctgctgc tgatcgcacg
     2521 tgcctttcag ctgcaggtgc tcgaggccga gttcctcaat cgcgaaggcg agtcgcgtta
     2581 cctgcgcgaa atgtcgatcg aagcctcgcg cggccgcatc ttcgaccgtg aaggtaatgt
     2641 cctcgcggtg tcgacgccgg tacgatcgat ctgggcggtt cccagcgatg tcaacgcgac
     2701 gccagcacag attcaggaga tcgccaagct gctcgggcag acgccggcgg agctgaagcg
     2761 caagctggcc agcgacaagc gtttcgtctt tctggcccgc cagatcgccc cggagaccgc
     2821 tgaccgagtc tcggccatga agattgccgg cgtctttcag gacaaggaat tccgccgctt
     2881 ctatcccggt gccgagttgt cggcgcacct cgtcggcttt accggcgtcg acggcaaggg
     2941 cctcgaggga atcgagctgg cgctgcaagc ggatctgacc ggcgtcgatg gcgcccgcac
     3001 ggtgatccgc aaccgccgcg gcgagatcgt cgaagacgtc ggtctgcgca agccggcccg
     3061 cgacggccgc gacgtcgcgc tggcgctcga cagcaagatt cagtacatcg cctacacgca
     3121 gttgcgccaa gcggtcaccg agcatcgcgc caaggctggc agcgtcgtcg tcctcgacgc
     3181 gcgtaacggc gaggtgctgg cgttggccaa ctggccgacc tataacccga acaaccgcca
     3241 gggtctgtcg ggtagcgcct tgcgcaaccg ggccgttacc gacaccttcg agccgggatc
     3301 gacgctcaag ccgtttacgg ttgcgctggc gctggaaaaa ggccgttacc gcttcaactc
     3361 gcggattgac accacgccgg gccggctgac gataggccgg gcgacgatct ccgatgccaa
     3421 gccgcatggc gtgctgaccg ttgccgaggt gttgcagaaa tcctcgaacg tcggcaccgc
     3481 gatgatggcc gccgatctgc cgtcgcgcga catgtggacg atgtacgacg cgctcggctt
     3541 cggcaagccg ccggcgctgg gattccccgg agaagtcggc ggacgcctgc gcgcctggaa
     3601 cggctggcgg ccaatcgaga aggcaacgat gtcctacggt catggcatct cgatgagcct
     3661 cgttcagctc gcccggtcgt ataccgtctt cggcaacggc ggtcgcctgc tgccgctgtc
     3721 gctgctcaag cgcgactacc aagcatcgat cggcacgccg ttactcgcgc cgcaggccgc
     3781 gcgagaagtg ctggcaatgc ttgaaatggc ggtacaagac ggcggcacgg cgccgaaggc
     3841 gcggatcccc ggctatcgcg tcgcaggcaa gaccggtacc gcccacaagc tcgaaggcgg
     3901 ctcctacgcc aacaagtacg tcgcctcatt cgtcggcctt gccccggttt ccagtccgcg
     3961 gctaatcgtc gccctgatga ttgacgaacc ttcggctggc aagtattacg gcggcgacgt
     4021 tgcggcgccg gtgttcgcgg ggatcatgga ggcctcgctg cgctcgctgg gcgtgatgcc
     4081 cgacctgact cgcctgcagg cgaccgcgcg ttcgacgccg ccgccggcaa cgccggaagc
     4141 gccggctcgg gaggcgcagg tggccgccgc caacctgacc gtggagcggc agctgtgatc
     4201 ggcgtcgtcg acagcatcgc cgaggaggcc atcgcccgcc ttgcggcaca gggcgttgcc
     4261 gttcggcgaa tcgcctgcga cagccgccgg gtcggaccgg gcgacgtctt catggcctgg
     4321 cccggtgccc gtcacgatgc gcggaactac atcatcgatg ccgtcggccg cggcgcctcg
     4381 ggggtcatct gggaggcgcg cgcctaccgc ttcccggcgg ccttgcgcgt cgccaacgtc
     4441 ggcgtcgagg atctgcagcg tcacgccggc gaaattgccc atctggtcta cggcaaacct
     4501 tcggaagcga tgcgcctgat cggcatcacc ggcaccaacg gcaagaccag ttgcagccac
     4561 tggcttgcag atgcgctggc cgccagcggc gagcgctgtg ccgtggtcgg caccctcggc
     4621 aacggtcttc ccggtcggtt ggccggtagc gccaatacca cgcccgacgc gatcacgctg
     4681 cacgcgctgc tggccgaatg gcagcgcgat tcgattgccg cctgcgcgat ggaagtatcg
     4741 tcgatcggcc tcgaccagca ccggctgtcg ggcgcccgct tcgatactgc ggtgttcacc
     4801 aacctgaccc acgatcacct cgactaccac ggcgacatgg atgcttatgc cgcggccaag
     4861 gcccggctct tcgactggcc cgacctgcgg gccgccgtgc tcaacctcga tgacccgttc
     4921 ggcgtggacc tggccgaacg cagcggtgcg ccgatcaaga tcggctacac cttgaccggg
     4981 aagagcgctc cggggctctc cgctatcgtc gcggcgcgcg acgtacacgc gacgccgacc
     5041 ggtaccgcat tcacgctggc ctgtggcgag gattcgcagc gcgtcgtcgt gccgctgatc
     5101 ggtcgtcaca acgtgcagaa cctgctcgct gtggctggcg ccttgctggt tggcgactac
     5161 ggcatcgagc ggatcgccgc gctgctgccg ctgttgaagg cgccgccggg tcgcctgcag
     5221 accctgggcg gctccggcgt gccgctggtc gtcatcgact acgcccacac gcccgacgcg
     5281 ctacgcaacg tgctcgaggc gctgcgcccg gtggccgagt cgcgcggcgg acggctaatc
     5341 gcggtgtttg gctgcggcgg aaatcgtgac aagggcaagc gcccgcaaat gggcgagatc
     5401 gctgcgcgac tagctgaccg cgtctggctg accagcgaca acccgcggca cgaggagcct
     5461 ctggcgatca ttgccgacat ctcggccggt gtgctcgcgg ccggcgtcaa gccgaccgtc
     5521 gatgccgacc gcgcccacgc ggtgcggacc gccattgcag ccgccgacgc gcgcgacgtc
     5581 gtgctgctcg ccggaaaggg tcacgaggcc taccaggaaa ttgccggcga acgccttcca
     5641 tacagtgacg cgatcaccgc gcagcgctcg ctggcgaggg agttgccgtg agcggcgcct
     5701 tcgacatgac ccttgccgaa gtcgcgcagg cgatcgccgc cgaacgcatc ggcgggaagc
     5761 cgcagaccct cgccaccggg gtggtgaccg acagccgtgc cgaggtcgcc ggcgccctat
     5821 tcgtcgcgtt gaagggcgag cacttcgacg gccacgaata cctcgcggcg gtgaaggcgg
     5881 ccggcgcggt cgccgcgatc gtcgatcgac gccgcgtcgc cgaactgtcc gtgatcggcc
     5941 tgccactaat cgccgtagcc gatccgcggc gcacgctcgg cgatctcgcc gccgtctggc
     6001 gcaagcgctt cgcgataccg ctggtcgggg tgaccggttc gaacggcaag accaccacca
     6061 aggaaatgat cgccgcgatt tttgcggcgc gggcgcgccg caccggcctc gccgatgcag
     6121 cggtggtcgc tacccgcggc aacctcaaca acgaaatcgg cgtgccgatg atgctgctgg
     6181 agctgacgcc gcaaacacgc tacgcggtga tcgaaatggg catgaaccat ccgggcgaga
     6241 tcgcgcgtct cgccgcgctg gccgcgccga cggtcgctgt agttaccaat gcccagcgtg
     6301 cccacctcga gggcatgggc tcgctcgccg caatcgtcgc cgagaaggca tcgattttcg
     6361 aagcgttgtc ggcagacggc gtcgccatca tcaatgccga cgacccgaac gccgagcgct
     6421 tcgcccgcgt tgcaggcgcc cgccgccagc tgctggcttc gctcgatggg agcggtgacg
     6481 tcgtcggccg ctatcgcccg actgcggccg ggtccacagt cgaaatcagc gatgtctcgg
     6541 gcaccgtcga aattgcgctg ccgctgtttg gtcgtcacaa cgcgcgcaac gccgtcctcg
     6601 ccaccgccgc ggcgctcgcc gccggtgcaa cgctgagcga tgccgccgcc ggtctggcga
     6661 cgctgcgcaa tgtcggtggg cggctgcggc tgcgcgccgc aaagggcggc gcccgcttgc
     6721 ttgacgacag ctacaacgcc aatcccgact cgatgcgggc ggcaatcgac gtgctcgccg
     6781 attctcccgg aacgcgaatt ctggtgatcg gcgacatggg cgagattggc cccgagcggc
     6841 tggccttgca cgccgagatg ggcgcctacg ccagggccag cggaatcgag cgattgttcg
     6901 cgctcggcga gtcggcgcgc gccgcggtcg aggcttttgg agccgccggc acgcactgga
     6961 agacgcccga agacttgagt gccgccctcc tgccgctgct ggacgccgat acgaccgtgc
     7021 tggtgaaagg ctcacgcttc atgcgcatgg aacgcgtcgc cgacgcgctc gccgacgagt
     7081 cgtcagcgac cacgcaaacc aaagaggacg cccgatgctc ctaatgattg cccagtggct
     7141 tgccgaggac gtgcgcgcct tcaacgtctt cacctacatc acgctgcgcg ccgtgctcgc
     7201 ggcgatgacc gcgctggtga tcgccttcat tgccggcccg cgcgtgatcc gctggctggc
     7261 cgagaaaaag attggccagg cggtgcgcag cgacgggccg cagacacacc tgatcaagtc
     7321 gggtacgccg actatgggcg gggtgctaat cctgatcgcg atcctgctga cgacgctgct
     7381 ttggggggac ctcaccaatc gttacatctg ggtggtggtc ttcgtcaccg cgggttttgg
     7441 cgcggtcggc tggatcgacg actggcgcaa ggtggttcag cgcaatccgc gcggattgcc
     7501 ggcacgctgg aaatttttct ggcagtcgat gattggcgtc gccgcggcgc tctacctcgg
     7561 tctttggccc actgtaccgg ccgcgcagga gctgatcgtg ccgttcttca agacgatcgc
     7621 ctatccgctc ggcgccttcg gcttcatcgt cctcacctat ctggtcatcg tcggcaccag
     7681 caatgcagtg aacctgaccg acggcctcga cggactggcg atcatgccga cggcgatgat
     7741 tgcgtcggcg ctggcgatct tcgcctacgt cgccggccac gtcgtctttg cccgctacct
     7801 cggcctgccg cacattcccg gcgccggcga actggctgtc ttctgcggcg cgatggccgg
     7861 cgcggcactc ggcttccttt ggtttaacac ctacccggcc gaagtcttca tgggtgacgt
     7921 cggtgcgctg gcgctgggtg ccgcactcgg caccgtcgcc gtcatcgtcc gccaggaaat
     7981 cgtgctggcg atcatgggcg gcgtcttcgt cgccgagacc ctgtcggtgg cggcgcaggt
     8041 cctctacttc aaggccaccg gcggcaagcg gatcttccgc atggcgcctt tgcaccatca
     8101 ctttgaactc ggcggttgga aagaaaccca ggttgtcgtt cgcttttgga tcatcacgat
     8161 catgctggtc ctgttcggtc tgtcgacctt gaagctgaga tgagactggc cggaaaaaat
     8221 atcttggtgc ttggcctcgg tgaatcgggg ctggcgatgg cgcgttggtg cgcccgccag
     8281 ggttcggccc tgcgcgtcgc cgacagccgt gcagcgccgc cgaatgcggc ggcgttggcg
     8341 cgcgaattgc ccgccgctca gctggattgc ggaccgttca ccgaggcgct gctcgacggc
     8401 atcgaccgca tcgcgctgtc gcccggcgta gcgttggccg agcccatcgt gcaggcggca
     8461 ttgcgccgcg ggattccggt cgtgtccgaa atcgagcttt ttgcagaagg cctgcgcggg
     8521 cagagcgaag ccgcccagat actggcgatc actggcagca acggcaagac gaccaccacg
     8581 gcgctcgccg ccgcactcag tacagcggcc gggcgggtga ccgtcgccgc cggcaacatt
     8641 tcgccctcgg cgcttgatgc gctgatggcc gcgctcgacg cgggagcgct acccgaactc
     8701 tgggtgctgg aactctccag cttccaactc gagaccacag caacgctcgg gccggtagcg
     8761 gcggctgtcc tcaacctcag cgaggaccac ctcgaccgct atgtcgacat ggccgcgtat
     8821 gcggcagcga aggcgcggat tctcgacggc gcggcaaccg ccgtgcttaa ccgagaagat
     8881 ccacgggttc tggcgatgcg ccgcgacggg cagaagaccg tcactttcgg cctcgacgtc
     8941 cccccgacgc ctgaggactt cgggctggtt gacggctgga tcgtccgcgg cggcacgcgc
     9001 ctcgtcgcac agaacctgtt gcgcctacat ggcagtcaca atgctgccaa tgcgatggcg
     9061 gcgctggcgc tgtgcgaggc cgccggcatc gagctatcga cggtgctgcc ggcactctgc
     9121 gccttcgccg gccttccgca ccgcgtcgag tggatcgccc gcatcgatgg cgtcgactac
     9181 tacgacgact cgaagggcac caacgttggc gctacgctgg ccgccttgca gggcatgggc
     9241 cggccggtcg cgatcattct cggcggcgac ggcaagggcc aggacttctc gccgctggtc
     9301 gaagcgatcg ctacgcacgc tcgacacgtc gcgctgatcg gccgcgacgc ggcccgactc
     9361 gaagcggtgc tggccggttg cggcgtgccg atgacccgct gtgtcgacat gccgtcggca
     9421 gtcgccgcct gcgctagcgc tgcccaggcc ggtgacgcgg tcctgctgtc cccggcctgc
     9481 gccagcctcg acatgtatcg cagctatgcg caccgcgccg aagtcttcgt cgcagcggtg
     9541 cgcagtctgc cgacttaccg ggcggaggcc gagcgatgat cggcgccgac ctcgcccata
     9601 cccaccgcga accggccgag atcgacatgg cgatcgtctg gtcagctttt gcgctgctgt
     9661 taatcggcgc cgtgatggtc tattcggcct cgatcgccat cgcccagggc agcgcattcg
     9721 ccgggtacaa acccgagttc tttctgatcc gccacgtggt cttcgtcggg accggcctgc
     9781 tcgccgcgct ggtggccttc cagattccgc ttaacctctg gcagaagatg gcgccggtgc
     9841 tttttctcgc cgccatcgtg ctgcttgcca tcgtgctgat tccggggatc ggcaagtcgg
     9901 tcaacggcgc gcagcgctgg ctgccgctgg gaatcatcaa cctgcagccg tccgagctga
     9961 tgaagctgtt cgtcgtgctc tacgccgccg attacacggt gcgcaagctg ccgctgatgc
    10021 acgacctcaa gcgcgccttc ctgccgatgg ccgccgtgct cgtggtggtc ggggggctgt
    10081 tgcttgccga gcccgacttc ggcgccttcg tggtgatcat cggcatcgcc atgggcgtac
    10141 tgtttctcgg gggcatgaag acccgtctgt tcgcgctgct gacggtaatc ctacttgttg
    10201 ccttcgttct gctgatctgg tcttcggcct atcggcgcga acggatactc ggcttcatgg
    10261 acccctgggc cgatccgctc ggtcgcggct accagctctc gcacgcactg atcgccttcg
    10321 gtcgcggcga agtgttcggc gtcggcctag gcgccagcgt cgagaagctc ttctacctcc
    10381 ccgaagcgca taccgacttc ctgcttgcag taatcgccga ggagactggt ttcctcggcg
    10441 tcgccctcgt cgtcgtgctg ttcgccgtgc tggtacagcg agcctttgcg gtcggccgtc
    10501 gggcgatcag ccttgagcgc ccctatgccg gcctcgtcgc gcaaggcttg ggcctctggc
    10561 tcggcgccca ggccttcatc aacatgggcg tcaacatggg cctcctgccg acgaagggtt
    10621 tgacgctgcc gttgatgagc ttcggcggct cgggaatcgt cgccaactgc atggccgtcg
    10681 ccgtacttct gcgcgtggat tgggaaaaca ggcagctcat gcgtggagga ggttggtgat
    10741 gccgtcattc ttgcctgttc cggcgatggc taacgtgcgc agcacgtcaa gcgcgcagcg
    10801 cggccatagc caagaacgcc aactcatgcg tggaggtcga tggtgaccgc cgctcccacg
    10861 ttgatgatca tggccggcgg aaccggcggg cacatcatgc ccggtctcgc cgtcgccgat
    10921 cttgtccggg cgcgtggctg gtcggtggtc tggctcggca atccggaggg tatggaggcg
    10981 cgtctggtgc cggccaaggg ctacccgctg gcgccggtca agttctcggc gctgcgcggc
    11041 aagggggtct tgcgcaagtt gctgctgccg cttgcgctgc tgcgaggctt ctacgattcg
    11101 ctgcgggcga tccgccgctg gcggcccgac gtggtgatcg ggttcggtgg ctacgtgagc
    11161 ttccccggcg gcatgatggc ctcgctgctc ggacgcccgc tggccatcca cgagcagaat
    11221 gcgatcgcag gactaaccaa ccgggtgctg gcacggcttg ccgatgccgt gctctgcggt
    11281 tttcccaagg cgctgccggg cgggcagtgg tgcggtaatc cggtgcgcgc ggcgatcgcc
    11341 gaactgccgg cgcccgagtt gcgctatggc gagcgcagtg gtccgctcaa cgtactggtc
    11401 gtcggcggct cgcttggtgc cgtggcgctg aacgacgtcg tgccgcgagc cgtcgcgcga
    11461 ttcgcagcgt cggagcggcc gcgcgtctgt catcaggcgg gcgaacgcca catcgatacg
    11521 ctgcgcgcgg gctacgccga agccggggta gccgccgagt gcattccctt tattgacgac
    11581 atggccgctg cctacgccga ggccgacctg gtgatctgcc gcgccggcgc gcttaccgtc
    11641 gctgaggtcg ccgccgccgg cgtcgcggcg atctttattc cgttcccgca cgcggttgac
    11701 gatcaccaga ccgtcaatgc gcgctttctc gccgacgcgc aggcggcgat cctgctgcca
    11761 cagagtcagc tcgacgccga tcggctggcg accacgctgc gcagtctaga ccgcgatgcg
    11821 cttatggcga tggcgatcaa ggcgcgcgcg ctggcgcggc cggatgcgac ggagacggtc
    11881 gcggcgacct gcgaacgcct cggaggcaag gcatgaaaca caagatcaag cacctgcact
    11941 tcatcggcgt cggcggctct ggcatgagcg gtatcgccga agtgctggcc aaccagggct
    12001 accgcgtcag cggttccgat ctctcggcaa gcgcgaccac cgaacggctg gccaaggcgg
    12061 gggttcgcat cgccattggc cacgacgcgg caaacattac cggcgccgat gccgtcgtgg
    12121 tctcgaccgc ggtcaaggct gacaaccccg aagtgcttgc cgcacgcgag gcgcatttgc
    12181 cagtggtgcc gcgggccttg atgctcgccg agctgatgcg catcaagcag ggcatcgcca
    12241 tcgctggcac gcacggcaag accaccacca caagcctcgt cgcgtcgatt ctcgccgagg
    12301 caggcttcga cccgaccttc gtcatcggcg gccgcctcaa cgccgcaggc gtcaatgccc
    12361 ggctcgggca gggcgagttt ctcgtcgccg aagccgacga gtcggacgcc tctttcctcc
    12421 atctgtcgcc agtgatctcg gtcgtcacca acatcgacgc cgaccatatg gagacctacg
    12481 ggcatgactt cagccgactc aagcagacct tcgtcgagtt cctcaaccgc ctgcccttct
    12541 atggcgtcgc cgtgctctgt gtcgacgacg ccaacatccg cgagatcatg ccggaagtgg
    12601 ccaagcagat cgtcggctac ggctttgccg cggaggcctc gatccgcgcc gaggatttgg
    12661 tcgccgacgg cgcgcagatg cgctttacgc tggtacggca gaacggcgtg acgacgcgga
    12721 cgccggtcgt cctcaacacg ccgggacggc acaacgtcct caacgcgctg gcggcgatcg
    12781 ccgtcgccac cgagatcggt gccgacgacg cggcgatcct caaggccctc gcggagttca
    12841 agggggtcgg ccggcgcttc cagcgctgtg gcgagattgc cgctattggc ggcggtcact
    12901 acacgctggt cgatgattac ggtcatcacc cggtcgaaat ggaagcgacg ctcgcggcgg
    12961 cgcgcggtgc attcccgggc cggcgcatcg tgcttgcctt tcagccgcac cgctacaccc
    13021 gtacccgcga ctgcttcgag gattttgtgc gcgtgctctc caatgccgat gccgtcgtgc
    13081 tgagcgaggt ctatgctgcc ggcgaagcgc cgatcgtcgc cgccgacggc cgctcgctgg
    13141 cgcgcgcact gcgcgtcgcc gggcgagtcg agccggtctt cgccgagacc atcgacgaga
    13201 tggaaaatgc aattggcgaa ctcgcacgcg acggcgacgt cgtgctggtc atgggcgccg
    13261 gatcgatcgg cggcctgccc tcgaggctgg gcgcatgttc atgagcgagc cgcttaactt
    13321 cgcacaactg cgcggtcgct ggcaggccga cgtgccgatg gggccgcagc tctcgtggcg
    13381 ggccggcggc catgcccggc gcgcctacgt accggccgac atcgacgatc tctgcgcctg
    13441 cattgccacg ctccccgccg acgagccgct gctcttcgtc gggctgggca gcaacctgct
    13501 ggtgcgcgac ggcggcttcc caggcacagt cttcctgctg cacggcacct tgcgccagct
    13561 gcagctcgat ggtgagttca tctacgccga agcgggcgtt accagcccga agctggcgcg
    13621 ctttgtcgcc cgtcagggcc gcgtcggcgg cgagttcctc gccggaattc ccggaaccat
    13681 cggcggtgcg ttggcgatga acgccggatg ctacggcggc gagacctggc ctctggtcga
    13741 ccgcgtgctg gtcgtcgatc ggcgcggcga gcgacgggtc atggccgcag ccgaattttc
    13801 gtttgcctat cgccatgtcg agccgcgaga caaggccgaa gcgccgctct ttttcctcgc
    13861 tgcctggttc cgctttccgc agggcgacgg cgaggcctcg cgccagcgaa ttgccgaact
    13921 gttggcgaag cgacaggcga cgcagcccct ggacctgccg aacgctggat cggtgttccg
    13981 taaccccgaa ggcgattttg ctgcgcggct aatcgaggca gccggactca agggtctgac
    14041 ggtcggtggt gcacaggtgt cgaccaagca cgccaacttc atcgtcaatt tcgccgggtg
    14101 tgccagtgcg gccgatatcg aggcgctgat cgagaccgtg cagaacgagg tacaggtgcg
    14161 ctttggcgtg agcctgcaac gcgaagtgaa aatcgtcggg gaacccaagc gatgagcggg
    14221 tcggatttcg gcaaggtggc agtgctgctc ggcggcgatt ccgccgagcg cgaggtatcg
    14281 ctgaacagcg gcggccgtgt gctcgcggcg ctcacgcgcg gcggcgtcga cgcgcatgcc
    14341 ttcgacccgg cgagccgtcc gctggccgag ctggcggaat tcgaccgtgt cttcatcgtg
    14401 ctgcacggcc ggcacggcga ggacgggacg atccaaggct tgctcgaact gatggggatt
    14461 ccctacaccg gcagcggcgt gctcgcgtcg gcggtgggta tggacaagtg gcgctgcaag
    14521 ctactctggc aagccgccgg gctgtcgctt cccgattacc gtctgctcga cgccaacagc
    14581 gatttcgacg ccatcgaggc ggcgctcgga ctccccttgt tcgttaagcc ggcctgcgaa
    14641 gggtccagcc ttggcgtgac catggtgaca cgccccggca tgctccgcag cgcctacgag
    14701 gaagcggcgg tcttcgaccc gctggtgatc gccgaaaagg cggtgctcgg cggcgaatac
    14761 acggtcggca tcctcggtag cggcgaatcg atgcaggcct tgccgatcat caagatcgag
    14821 cctgcaaccg ccttctacga ctacgaggcg aaatacacgc gcgacgacac gcgttacctc
    14881 tgcccggccg gcctttcgcc cgagcgggag gccgaactgc gcgccgagtc gttgcgtgcc
    14941 ttccggatcc tcggtgggcg cggttggggc cgggtcgact tcctgatgga tggcgatggc
    15001 aaggcctact tcctcgaagc caataccgcg cccggcatga ccgaccactc gctggtgccg
    15061 atggctgccc gggcggccgg tatcgactac gacgaacttg tgctgcgcgt cctagcgctg
    15121 gcgcagacag gcggaggcta ggccatgtgg caccgcccga acctgctgac cgcgaccgcc
    15181 gacctgctct tctttgccgg cgccttcgtg ctcgcgatcg ttgccacgct ggccctggcg
    15241 cgcgcaccgt ggtttccctt gcgcgaggtg gtcgtcagcg agccgctgcg cgagacccgc
    15301 cggccagaga ttgaagcggc gctgcgcgac tcgctgcacc cctcgggcat ctggacagtc
    15361 agcaccgagc gtgtgcggac ggcgatcgag cgcctgccct gggtgcgccg cgccgaggtg
    15421 cggcgcgtct ggccccaccg gctggtgatt catatcgagg agcatcgcgc cgtggcgcgt
    15481 tggggtgcag gcaacgcaca gctgcttaac gatcgtggcg aggttttttt cgccagcacc
    15541 agtcgtccgc caaggctcga actaatcggt cccttaggca gtgcggccga ggtgctcgcc
    15601 cgccaccgcg aattcggcaa cttgctggcg ccgctcggtg cgccgatcgc caggctcgaa
    15661 ctgtcgtcgc ggctagcctg gcgcattcgg ctcgacgacg ggctgctcat cgaattggga
    15721 cgcgagcagg cgcgtgcccc cgtcgccgct cgcctgaagc gcttcgcagc cgcctatcgc
    15781 gaactgatcg ccgacgcatc gcggcgggcc gcggtggtcg acctgcgcta tgtcaacgga
    15841 tttgcaatgc gcctcgccgg caatggcgcc ggcgcggtca actgaggaag tcatgggcaa
    15901 ggaaagcagg aacctgatcg tcggcctcga catcggcacc tcgaagatcg tggcgctggt
    15961 ggccgaactc gacgacaacg gggcaatcaa cgtgctcggc gtcggcacgc aggattcgaa
    16021 aggcctgcgc aagggcgtgg tcgtcaacat cgaggagacc gtcggcgcga tttcgcgtgc
    16081 gatccaggaa gtcgagctga tggccaatcg acaggtggcc gaggtctaca ccggcatcgc
    16141 cggcagccac atcaagagct tcaattccag tggcacggtg gcgatcaagg acaaggaagt
    16201 cacaccaatg gacgtcgagc gtgtgatcga agtcgcgcgt gcgatgccca tcccggccga
    16261 acagcagatc ctgcatatcc tgac
//
LOCUS       DATDAA010000383         4819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_202860,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000383 DATDAA010000000
VERSION     DATDAA010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202860"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VIS73_11465"
     CDS             complement(<1..937)
                     /locus_tag="VIS73_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763815.1"
                     /translation="MDNSLGFLREWLPFARNDRTDRVTAALQEWLAGLGQMAESERAR
                     LLTDRLKQLLAAQENPLAQAKILAAVDAEAGLLLPPLEMTVAAAPLPLDDDVSEAALA
                     GDNLLKGLAAGYLQLAAQLMGRRSASGVAETLGRALTAAAATLLRRQLLAYRAYAPAS
                     ASSWLAFHRIYGLAQAHKTQIPGAFAQIELHYHAALLLALADPMKFSREALPLLQKLS
                     EGMARYVRLYPTAGHKLPETASLFLVLPSEGRPARPLARNESQPLDYDGYIVDCNPAV
                     GALRKEIARHELAGGRSANDDADLTVMAALLSMWRG"
     gene            complement(1064..2368)
                     /locus_tag="VIS73_11470"
     CDS             complement(1064..2368)
                     /locus_tag="VIS73_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY5763816.1"
                     /translation="MSAIEKIPGLPPVGDQGLLRFLTCGSVDDGKSTLIGRLLFDTKA
                     ILADTLSHIETTSQRRGLSAVDLSLLTDGLQAEREQGITIDVAYRYFATGTRKYIIAD
                     APGHEQYTRNMVTAASTAHLAIILVDARRGVVTQTRRHSLVAQLMGIRHLVVAVNKMD
                     LVDYDEAVFQKIKDDYLDFAHKVGLTDGETDIRFLPLSALQGDMVVDRGSRLTWHNGP
                     TLLEILESTPCAHCEHSEPFRFPIQYVIRPQASGDQQLHDYRGYAGRVESGTVKVGDA
                     VTILPSGRSTTIQSIQIGGQEIAAAVPDQSVTLLIADDIDISRGDMIVRSHNAPQTVK
                     ELEATLSWLAESPLDRNRTYLIRHTTREVKAKLAAIDYRLDVNTLEHLGAEQLAMNDI
                     ARVRFRLAQPLFVDSYENNRATGAFIVIDEATNNTVGAGMIA"
     gene            complement(2368..3309)
                     /gene="cysD"
                     /locus_tag="VIS73_11475"
     CDS             complement(2368..3309)
                     /gene="cysD"
                     /locus_tag="VIS73_11475"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004694369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY5763817.1"
                     /translation="MSTLQATATSAATVDTRVSHLDWLESEAIHIMREVAGQCRNPAL
                     LFSGGKDSICLLRIAEKAFRPARFPFALLHIDTGHNYPEVIDFRDRRAAELGERLIVR
                     TIEDSMARGSVVLKHADESRNKHQSVSLLEAIEEFGFDACIGGARRDEEKARAKERIF
                     SFRDEFGQWDPKNQRPELWDLYNARVHKGENIRAFPISNWTELDVWQYIARESLELPP
                     IYFAHEREIVRRNGTLVPVTPLTPARPGDTVETLSVRFRTVGDITCTAPVLSTAATVD
                     DIIAETATTTITERGATRLDDQASDAAMEQRKKEGYF"
     gene            complement(3306..4007)
                     /locus_tag="VIS73_11480"
     CDS             complement(3306..4007)
                     /locus_tag="VIS73_11480"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY5763818.1"
                     /translation="MQTRVAAKAAAVEQLLGEISEEFGKVAFASSLGAEDQVLTDILI
                     KSGLPIEIFSLDTGRLPTETYDLIAAVEGRYETKLSIYYPKHEAVEQYVRSEGINGFY
                     ESVAQRKSCCYVRKVEPLKRALAGKDAWITGLRAEQAATRSALPVREFDAGNGLEKFN
                     PLADWSEQEVWAYIREHEVPYNALHDKHFPSIGCSPCTRAIAAGEDVRAGRWWWESPE
                     SKECGLHVKKVGAQQ"
     gene            complement(4068..4589)
                     /locus_tag="VIS73_11485"
                     /pseudo
     CDS             complement(4068..4589)
                     /locus_tag="VIS73_11485"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
BASE COUNT          781 a         1449 c         1665 g          924 t
ORIGIN      
        1 ggccgcgcca catcgagagc agcgcggcca tcaccgtcag gtcggcatcg tcgttggcgc
       61 tgcggccgcc ggccagttca tggcgggcga tttccttgcg cagggcgccg acggccgggt
      121 tgcagtcgac gatgtagccg tcgtagtcca gcggctgtga ttcgttgcgc gcgagcggcc
      181 gcgcaggccg gccttcgctc ggcagcacga ggaagagcga agcggtttcg ggcagcttgt
      241 ggcctgcggt cggatagagc cggacgtagc gggccatgcc ttcggagagc ttctgcagca
      301 gtggcagggc ttctcgcgag aacttcatcg gatcggccag cgcgagcagc agcgcggcgt
      361 ggtagtgcag ttcgatttga gcgaaggcgc cgggaatctg cgtcttgtgc gcctgcgcga
      421 ggccgtagat gcggtgaaag gcgagccagg acgatgctga cgccggcgcg taggcacggt
      481 aggcgagcag ttgccggcgc agcagcgtcg ccgcggctgc tgtcagcgca cggcccaagg
      541 tttctgcgac gcccgaagcc gaccggcgac ccattagctg tgcggcgagc tggaggtagc
      601 cagccgccag ccccttgagc aggttgtcgc cggcgagtgc ggcctccgac acatcatcat
      661 caagtggcag cggtgcggcg gcaacggtca tttcgagcgg cggcagcagc aggcctgctt
      721 cggcatcgac ggcggcgagg atcttcgcct gcgccagcgg gttctcctgg gcggcgagca
      781 gctgcttcag acggtcggtg agcaggcgcg cacgctcgga ctcggccatc tggcccagcc
      841 cggcaagcca ctcctgcagt gcggcggtaa cccggtcagt acggtcgttg cgcgcgaacg
      901 gcagccactc gcgcaggaat cccagcgaat tgtccatcgg cggatttgtt gttctccctg
      961 atgccgccga tgatcgctgc tattccgatg tcctgcaagc tgccgcgccg gccgcgccgg
     1021 cactattgcg cagtggctga cgcggcaggc gcgaatttgc cgctcaggcg atcatgccgg
     1081 cgccgacagt gttgttggtc gcctcgtcga tgacgatgaa ggcgccggtt gcccggttgt
     1141 tctcgtagct gtcgacgaac agcggctgcg ccaagcgaaa gcgaacccgc gcgatgtcgt
     1201 tcatcgccag ctgctcggcg ccgaggtgct cgagcgtatt gacgtcgagc cggtagtcga
     1261 ttgcggcgag cttggccttg acctcgcgcg tggtgtgccg gatcaggtag gtccgattgc
     1321 gatcgagcgg tgattcagcc agccagctca gcgttgcctc gagttccttc actgtctgcg
     1381 gcgcgttgtg gctgcgcacg atcatgtcgc cgcgcgagat atcgatgtcg tcggcaatca
     1441 gcagcgtcac cgactggtcg ggaacggccg cggcaatttc ctggccgccg atctggatcg
     1501 actggatcgt cgtgctgcgc cctgatggca ggattgtcac cgcgtcgccg accttgaccg
     1561 tgcccgactc gacccttccg gcgtagccac ggtagtcatg cagctgctgg tcgcccgagg
     1621 cctgtggccg gatcacgtac tggatcggaa agcggaacgg ctcgctgtgc tcgcaatggg
     1681 cgcagggcgt gctttcgagg atttcgagca gcgtcggccc gttgtgccag gtaagcctcg
     1741 atccgcggtc gacgaccatg tcgccctgca gtgccgacag cgggaggaag cggatgtccg
     1801 tttcaccatc ggtcaggccg accttatgcg cgaagtccag gtagtcgtcc ttgatcttct
     1861 ggaacaccgc ctcgtcgtaa tcgactagat ccatcttgtt cacggcgacg accagatgac
     1921 ggattcccat cagctgcgct accagcgagt ggcggcgcgt ctgcgtcacc acgccgcggc
     1981 gggcatcgac gaggatgatt gccaggtgcg cagtggaggc ggcggtcacc atgttgcgcg
     2041 tgtactgctc gtgccctggc gcatcggcga tgatgtactt gcgcgtgccg gtggcgaagt
     2101 agcggtaggc gacgtcgatg gtgatgccct gttcgcgctc ggcctgcagg ccgtcggtca
     2161 gcagcgagag atcgacggcc gagaggccgc ggcgctgcga ggtggtctcg atatgcgaca
     2221 gcgtgtcggc gaggatcgcc ttagtgtcga agaggagccg cccgatcagc gtgctcttgc
     2281 cgtcatcaac gctgccgcag gtgagaaagc gcagcagacc ctggtcgccg acgggtggca
     2341 gccccggaat tttctcgatc gcgctcatca aaagtaacct tcctttttgc gttgctccat
     2401 ggcagcgtca gatgcctgat cgtcaaggcg cgttgcgccg cgttcggtaa tcgtggtcgt
     2461 tgccgtctcg gcaatgatgt cgtcgacggt cgcggctgtg gacagcaccg gcgccgtgca
     2521 ggtgatgtca ccgaccgtgc ggaagcgcac cgagagtgtt tccaccgtgt cgcccggccg
     2581 ggccggcgtc agcggcgtca ccggcaccag cgtgccgttg cggcgaacga tttcgcgctc
     2641 gtgcgcaaag tagatcggcg gcagttcgag gctctcgcgg gcgatgtact gccagacgtc
     2701 gagttcggtc cagttcgaga tcggaaaggc gcggatgttt tcgcccttgt gcacgcgggc
     2761 gttgtagaga tcccacagct cgggccgttg gttcttcggg tcccactggc cgaactcgtc
     2821 gcggaaggaa aagatgcgtt ccttggcgcg tgccttctcc tcgtcgcggc gggcaccgcc
     2881 gatgcaggcg tcgaagccga attcctcgat cgcttcgagc agcgacaccg actggtgctt
     2941 gttgcgcgat tcgtcggcgt gcttgagcac gacgctgccg cgggccatcg aatcctcgat
     3001 cgtgcggacg atcagccgtt cgccgagttc ggcggcgcgg cgatcgcgga agtcgatcac
     3061 ctcggggtag ttgtggccgg tgtcgatgtg cagcagcgcg aagggaaagc gcgccgggcg
     3121 gaaagccttt tcggcaatgc gcagcaggca gatcgaatcc ttgccgcccg agaacagcag
     3181 ggccgggttg cggcactggc cggcgacctc gcgcatgatg tgaatggctt ccgactccag
     3241 ccagtccaga tgcgagacac gggtgtcgac tgtcgcggcc gaggtcgcgg tcgcctgcag
     3301 ggtgctcatt gctgtgctcc gactttcttc acgtgcaggc cgcactcttt tgactcgggc
     3361 gactcccacc accagcggcc ggcgcggaca tcttcgccgg cggcgatggc gcgggtgcag
     3421 ggggagcagc cgatgctcgg gaagtgcttg tcgtgcagcg cgttataggg aacctcgtgc
     3481 tcgcggatat aggcccagac ttcctgctcg ctccagtcgg ccagcggatt aaatttctcg
     3541 aggccgttgc cggcgtcgaa ttcacgcacc ggcagcgcgc tgcgggtggc ggcctgctcg
     3601 gcgcggagcc cggtgatcca ggcgtccttg ccggccagtg cgcgcttcag cggctcgacc
     3661 ttgcgcacgt agcagcagct cttgcgctgt gccaccgatt cgtagaaacc gttgatgcct
     3721 tcgctgcgaa cgtattgctc gacggcctcg tgcttcgggt agtagatcga cagcttagtc
     3781 tcgtagcgcc cctcgactgc ggcgatcagg tcgtaggttt cggtcggcag acgcccggtg
     3841 tcgagcgaga agatttcgat cggcaggccg gacttgatga ggatgtcggt cagtacctgg
     3901 tcttcggcgc cgaggctgga ggcgaaggcg accttgccga actcttcgct gatctcgccg
     3961 agcagttgct cgaccgcggc cgccttggcg gcgacgcggg tctgcagcgg cgcgttgaga
     4021 tcgatcagcg gcgcgctctg gctggcgcgg cggcggaaca gcgggcgcgg ttggtcggcg
     4081 gcggcctggt aggcatcggg gaagtcggtg aggctggcga ccgcttcctc gatgtcgcgg
     4141 tcggcgcgca gcgcgaagga gtcgaagcca caacgcttca ggtagaacag ctggtcctgc
     4201 agcacgtcgc cgagggcgcg cacttcacct tcgtagccgt agcgctcgcg cagcaggcgg
     4261 gccgtggagt acccgcggcc gtcggcgaac ttcgggaagt tgatgccgat gacctggaag
     4321 tagtgcaagt cgtcggccac cgcttcgacc acctcgttgc tgtcgagcca gaggccgatg
     4381 cgcttgtagc gcgagatgat ttcttgtttg cgcgccgtcc acagcgacag cgggaagagg
     4441 tgctggccgg tgcttagttc gaacgatgct ggcgtgtcgc catcgttcag tcgaatctgg
     4501 ttccagttgt cgtcgatgac ctgctctgcg cgaatgatct ttgccatttg ggtgcctgcc
     4561 ttttcatgaa gtgtttcgat ggaaggcatg ctaacaatgc gcaaaagaaa agaaaaagaa
     4621 taaaaagtca gatataaata accggacgat ctaaacggct ggggccggtc gccttcgcaa
     4681 gaatcgacga caagcaactg taaataaaag aattattgtt cagacgccgg cgcagaacga
     4741 cgaaggggcc gtcgcgcgac ggccccttcg tcgttctgcg ccggcgtctg aacaataatt
     4801 cttttattta cagttgctt
//
LOCUS       DATDAA010000384         2626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_202885,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000384 DATDAA010000000
VERSION     DATDAA010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202885"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VIS73_11490"
     CDS             <1..756
                     /locus_tag="VIS73_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY5763819.1"
                     /translation="ADAITVASTTSTEQSGLFGHLLPAFTAATGIEVKVVALGTGQAL
                     DVGRRGDADVLFVHDRNAEEKFVADGFGINRRDVMYNDFVIVGPKADPAGAKGAADAV
                     DALKRIAASGAAFASRGDKSGTHSAELRFWKAADIDPKSLAGYKELGAGMGPTLNTAA
                     GMNAYTLSDRGTWLSFKNRQELEVVFSGDPRLFNPYGVILVNPARHPHVKQAAAQRFI
                     DWIVSPVGQSLIASYRIAGENLFFPSATQQAVK"
     gene            1121..1363
                     /locus_tag="VIS73_11495"
     CDS             1121..1363
                     /locus_tag="VIS73_11495"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HEY5763820.1"
                     /translation="MDDGRSSGRLIGDGDSGLQLNDTAVEILLRCNGSKNVERLIVEM
                     VELYSGSDECEIASGVQEFLDEALLRGWIDRSADLG"
     gene            complement(1387..>2626)
                     /locus_tag="VIS73_11500"
     CDS             complement(1387..>2626)
                     /locus_tag="VIS73_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY5763821.1"
                     /translation="NAQFEQIFRQSVKAVLDHATVRGSDPLALVTAKGLTLQTKVIPG
                     ALMKTPLRVFTFPRAEAVRAEKRPPVVEQRESSLDQLDTGDPHVRAVLHKVRRVLGKD
                     IPVLIHGETGAGKELLARAIHDDGPRFDKPFVAVNCAAIPEGLIESELFGYEEGAFTG
                     AKRKGNGGRIQQADGGTLFLDEIGDMPAALQARLLRVLQERVVNPLGSTKSIPVDIVL
                     VCATHRRLKELVAEGTFREDLYYRLNGLTVNLPPLRSRTDLAPLVALILTRLQPPNTP
                     LPQIEPEVLELFVRHPWPGNIRQLSNLLRTALVMAGDSDCITVDHMPDDFLEEFEAVE
                     QVPSQAHRVSAPTATAGATASASPTGLAEAGAGDLESTTLNLIQRALEQHAGNVSAAA
                     RQLGISRNTLYRKLKQIWKL"
BASE COUNT          495 a          853 c          806 g          472 t
ORIGIN      
        1 gccgatgcaa tcaccgtggc ctcgacgacc tcgaccgagc agtctggcct cttcggccac
       61 ctgctgccgg ccttcaccgc cgccactgga atcgaagtca aggtcgtcgc cctcggcacc
      121 gggcaggcgc tcgacgtggg gcgacgcggt gacgccgacg tgctgttcgt gcacgatcgc
      181 aacgccgagg agaaattcgt cgccgacggc ttcggcatca atcggcggga cgtgatgtac
      241 aacgatttcg tgatcgtcgg ccccaaggct gacccggcgg gggccaaggg cgcagccgat
      301 gcagtcgatg cgctgaagcg gatcgcggcc tctggtgccg ccttcgcctc gcgcggggac
      361 aagagcggca cgcactcggc cgaactgcgc ttctggaaag ccgccgacat cgatccgaag
      421 agcctcgccg gttacaagga actcggcgcc ggcatggggc cgaccctcaa caccgcagcc
      481 ggcatgaacg cctacacgct ttccgaccgt ggcacttggc tttccttcaa gaaccgtcag
      541 gaacttgaag ttgtcttttc cggtgaccca cgcctcttca acccctatgg ggtcatcctc
      601 gtcaatcccg ccagacatcc acacgtgaag caggcagctg cgcaacgctt catcgactgg
      661 atcgtctcgc cagtcgggca atcgttgatc gcgtcctaca ggatcgccgg cgagaatctg
      721 ttcttcccga gcgccacaca gcaggcggtc aagtaaccgc gccaaccggt cggtcgcaat
      781 tcgcaagaat ggcctgcccc ggttattcaa tacccaatca agccaagcgt tgtccgttga
      841 tcctgccagc gcaaatacat ctcgcggatc ggctttgcgc cagtcgcccg caacagctgt
      901 tcaaccatgt gacagtctcg gctcgcatcc cgcgacggaa tttcgggcca acacagggat
      961 ttaacgggaa tcggtattcg acagccctcg aatgctttgc ggaacgaaaa ctgctttgac
     1021 aaccgcatta gcgcaaaacg gagcctcgcg aatgggatcg ggcgtaccca agctgtcatc
     1081 tgccgaccgg cctcgccttt cagcgaattt ccgcctctgc atggacgacg ggcggagctc
     1141 tgggcgactg atcggcgacg gagacagcgg tctgcaactc aacgacaccg ccgtcgagat
     1201 cctcttgcgc tgcaacggca gcaagaacgt cgaacgcctg atcgttgaga tggttgagtt
     1261 gtattcgggc agcgacgagt gcgaaatcgc cagcggcgtc caggagtttc tcgacgaagc
     1321 actgctccgt ggctggatag accgtagcgc agacctcggt tagtcagtca acctgcgggc
     1381 caccgctcag agtttccata tctgcttgag cttgcggtac agcgtgttgc ggctgatgcc
     1441 gagctggcgt gccgccgccg agacgttgcc ggcgtgctgc tcaagcgccc gctggatgag
     1501 gttcagcgtc gtcgactcaa ggtcgccggc accggcttcg gcaagcccgg taggcgaagc
     1561 actggccgtc gccccagcag tcgccgtcgg cgcgctgacc cggtgcgctt gagacggcac
     1621 ctgctcgacc gcctcgaact cttcgaggaa gtcatcgggc atgtggtcga ccgtgatgca
     1681 atcgctgtcg ccagccatga ccaacgcggt tcgcagaagg ttcgagagct ggcgaatgtt
     1741 tcccggccaa ggatggcgga cgaacaactc aaggacttca ggttcgatct ggggcagcgg
     1801 cgtgtttggc ggctgcagac gagtcaggat cagcgccacc agcggcgcca ggtcggtccg
     1861 cgagcgcagc ggcggcaaat tgacagtcaa accgttcaat ctgtagtaga ggtcttcgcg
     1921 gaacgttcct tcggcaacga gttccttgag tcgccgatgg gtcgcgcaga ccagtacgat
     1981 gtcgacggga atcgatttcg tcgatcccag cggattgaca acccgctcct gcaacacgcg
     2041 aagcaaccgc gcctgcaaag cagccggcat gtcgccgatt tcgtcgagaa acagcgtacc
     2101 gccatcggcc tgctggatac gcccgccgtt gcccttgcgc ttcgccccgg tgaaggcacc
     2161 ttcctcgtaa ccaaaaagct cggactcgat gagcccctcg ggaatcgcgg cgcaattgac
     2221 agcgacgaag ggcttgtcga agcgcggccc gtcgtcgtgg atggctctcg ccagcagttc
     2281 cttgccggca ccggtctcgc cgtggatcag caccggaatg tccttgccca gcacgcgccg
     2341 gaccttgtgc aggaccgcgc ggacatgggg atcgcccgta tcgagctggt cgagcgaact
     2401 ctcgcgttgc tcgacgaccg gcgggcgctt ctccgcgcgc accgcttcgg cacgggggaa
     2461 ggtgaacacc cgaagcggcg tcttcatgag ggcgccggga atcaccttgg tctgcagcgt
     2521 cagccccttc gcggtcacca acgccaatgg atcgctgccg cgtaccgtcg cgtggtcgag
     2581 caccgccttc accgactgcc ggaagatctg ctcgaactgc gcgttg
//
LOCUS       DATDAA010000385         5238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_202983,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000385 DATDAA010000000
VERSION     DATDAA010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202983"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VIS73_11505"
     CDS             <1..750
                     /locus_tag="VIS73_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HEY5763822.1"
                     /translation="EVNEAVKSRGAFLHNIMPLISDPAHGTVFGLNGQRGPTPAELKE
                     LQDACEGDMQLMRHCRQCRADAVGMLGEDRGAEFSSDRIEAIAAEVDATTVSESRAAY
                     RDFVERERADQQAAAAQARTALLSADETRLVAVATKGSGRINQHFGHAEEFQVYEVSA
                     AGAKFVGHRKVSLYCQGGFDDDEAMPGIVDALAGCSAVLVAKIGHCPKKDLAAAGIEA
                     VESYAHEYIEASALAWFREHLNATRVAATAA"
     gene            765..1175
                     /locus_tag="VIS73_11510"
     CDS             765..1175
                     /locus_tag="VIS73_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY5763823.1"
                     /translation="MCVTLSESAERFMKRILRFSGAPPGSGFRLVVRSGGCSGFDSQF
                     SVEPEPLDGDAVVDQNGWRLFLEAETCALLRGATIEFAESRIDGGLRYSLPGGAASCG
                     CSSGSGASKSTTVGIQFMRRPGTCSKPSGASGRG"
     gene            1178..1471
                     /locus_tag="VIS73_11515"
     CDS             1178..1471
                     /locus_tag="VIS73_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HEY5763824.1"
                     /translation="MLARDSDEVELVGDPAFEFGEKVRALKNVRNDGTYYGRDIGDLL
                     VKKGDVGYVKTVGTYLQQFYIYGVDWIDRGVVVGMKARELESLDSNAGGSSRF"
     gene            1490..1699
                     /gene="nifT"
                     /locus_tag="VIS73_11520"
     CDS             1490..1699
                     /gene="nifT"
                     /locus_tag="VIS73_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nitrogen fixation protein NifT"
                     /protein_id="HEY5763825.1"
                     /translation="MKVTVRRESDGDFSVYVAKKDLEARVVASERDALWGGTVTLDNG
                     WRLEMPEMAADTRLPLTVEARKLAD"
     gene            1704..2603
                     /locus_tag="VIS73_11525"
     CDS             1704..2603
                     /locus_tag="VIS73_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEY5763826.1"
                     /translation="MTSQSIERLAATLVAGKLAPYLGPELLLLEAANAWLPVRPETLA
                     ERLAKRSAVPGRLRRNLTAAAQYIENFRHRRVLRGLVREVFTDRALPTRLHHLLAALP
                     LPLVVDVGYDAAMAAALAEHRDPSEWGQVQAVSRADEKADVWTQHYAAGGERVDPRAG
                     RGWRTLLYKPFGSILPEANFLVSDSDFVEVLTEIDIQTPIPENVQLRRSSLGFLFVGC
                     RFDNQLARTYARQVSKRSSGPHFAVMQGELTANEARFLAEQGIQRIDMATADFVLQLG
                     AAIEKALIAGAKRNQEAVAPVAM"
     gene            complement(2573..2983)
                     /locus_tag="VIS73_11530"
     CDS             complement(2573..2983)
                     /locus_tag="VIS73_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HEY5763827.1"
                     /translation="MAITLNWDSKFEIGHERIDDEHRIFLGLIRDLSNEASPEGDRTR
                     ATRLLLEIYKYADFHFVSEENVMADIAYPELDAHRRLHLALLAELRDCMFRFRSADGT
                     AEQAVEFLFTWFALHTTREDKRIADYIATGATAS"
     gene            complement(3118..4041)
                     /gene="draG"
                     /locus_tag="VIS73_11535"
     CDS             complement(3118..4041)
                     /gene="draG"
                     /locus_tag="VIS73_11535"
                     /EC_number="3.2.2.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047407 - ADP-ribosyl-[dinitrogen
                     reductase] hydrolase activity [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyl-[dinitrogen reductase] hydrolase"
                     /protein_id="HEY5763828.1"
                     /translation="MKPAAALTLLDRARGAYLGLAIGDALGATVEFMTSGEIRHQYGV
                     HREIIGGGWLRLKPGQVTDDTQMSLAMGEAIINAGAFDLKAIADAWAAWLRSRPIDVG
                     NTCRRGIQRYLIDGSLSKEACEGDGGNGALMRNLPVILLAHDDPQRRVEWSLQQAHLT
                     HHHPLSDAAVVAFAHLTADLLAGAAPEVIRDHADTLVASHRCFRFDPYPRRSSGYVVD
                     TVQTTLDAFFTTGDFEACLLRAVKWGGDADTNGAIAGWLAGAAYGAGAIPARWLKKLD
                     PDVRASVDNQANLLVAMNGSRPESVIEMTQT"
     gene            complement(4038..4865)
                     /locus_tag="VIS73_11540"
     CDS             complement(4038..4865)
                     /locus_tag="VIS73_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--dinitrogen-reductase
                     ADP-D-ribosyltransferase"
                     /protein_id="HEY5763829.1"
                     /translation="MTPPPSKDVGEAQRWHTSNLIGIPTALLASPAFNAEPPDVHISG
                     VRETNRTLFAMLLEATNIEESMLAFQIYMRTVFELDLPASRDVQGRRRYRASYLRLLR
                     GWGFDANGREGAVMKGWVESRFGLLPTFHKAPLRHYPSAGWMTYLEEKMSARFTNNSI
                     NAQLDLLYEYSQWMFARWLLPGARHLRLYRGVNDLAEHQVVEWLGKRLAIARQNNLVS
                     FTDRRDIADQFGDSILEVDVPIAKIVFANSLLPGSTLKGEGEFLVLGGDYQVKVATL"
     gene            complement(4879..5085)
                     /locus_tag="VIS73_11545"
     CDS             complement(4879..5085)
                     /locus_tag="VIS73_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEY5763830.1"
                     /translation="MPLFDFHCAKCECTSEMLVRHDAEPACPQCGHPQLQKLMAKPAA
                     PGTTKELIGKARRRAKAEGHFSNY"
BASE COUNT          934 a         1609 c         1746 g          949 t
ORIGIN      
        1 gaagtcaacg aggcggtcaa gtcacgcggc gcctttctcc ataacatcat gccgctgatc
       61 tccgacccgg cgcacgggac ggtcttcggg ctgaatggac agcgcgggcc gaccccggcc
      121 gaactcaagg aattgcagga tgcctgcgaa ggcgacatgc aactgatgcg gcattgtcga
      181 caatgccgcg ccgatgccgt gggaatgctc ggcgaagatc gcggcgcgga attctccagc
      241 gaccggatcg aagcaatcgc cgccgaggtc gacgcgacaa ccgtcagcga atcgcgcgcg
      301 gcctatcgcg attttgtcga acgcgaacgc gccgaccagc aggccgccgc cgcgcaggcg
      361 cgtacggcgc tgctttctgc cgacgagacc cgtctcgtgg ccgtagcaac caagggctcc
      421 ggccgaatca accagcattt tgggcatgcc gaggagttcc aggtatacga ggtctccgct
      481 gccggcgcca aattcgtcgg tcatcgcaag gtgagtctct actgtcaggg cggtttcgac
      541 gacgacgaag cgatgccggg catcgtcgat gcgctggccg gctgcagcgc ggtgctagtg
      601 gcgaagatcg gccattgccc gaagaaggac cttgccgcgg ctggcatcga ggccgtcgag
      661 agctacgccc atgagtacat cgaggcctcg gcgctggcgt ggttccgtga gcaccttaac
      721 gcgacgcgag tggccgcgac ggccgcctga cccgggagaa caccatgtgc gtgacattga
      781 gcgaatccgc cgagcgtttc atgaagcgaa tcctgcgctt ctcaggggcg ccgcccggat
      841 caggtttccg cctagtcgtg cgctcgggcg gatgctctgg tttcgactcg cagttctccg
      901 tcgagcctga gccgctggac ggtgacgccg tagtggatca gaacggctgg cgcctgttcc
      961 tcgaagccga gacctgcgcg ctcttgcgtg gagccaccat cgaatttgcc gagagccgaa
     1021 tcgacggcgg cctacgctac agcctgccgg gcggagcggc ttcctgcggt tgtagctcgg
     1081 gcagcggcgc cagcaagtcg acgacggtcg gtattcaatt catgcggcgg ccgggcacct
     1141 gcagcaagcc atccggcgca tcgggcaggg gttgatcgtg ctggcgcgtg acagcgacga
     1201 ggtcgagctg gtcggcgatc ccgcgtttga attcggcgag aaggttcgcg cgctcaagaa
     1261 cgtgcgcaac gacgggacct actacggccg cgacatcggc gacctgctgg tgaaaaaagg
     1321 cgacgtcggc tacgtgaaga cggttggtac ctacctgcag cagttttaca tctacggcgt
     1381 cgactggatt gatcgcggcg tcgtggtagg catgaaggca cgcgagcttg aatcgctaga
     1441 ttcaaacgcc ggcggttcat cgaggttctg agcgaaggga gcgaacaata tgaaggtaac
     1501 ggtacgcagg gaaagcgacg gcgacttctc cgtctacgtg gcaaagaagg atctcgaggc
     1561 ccgggtggtc gccagcgagc gcgacgccct ctggggcggc acggtgacgc tcgacaacgg
     1621 ctggcgtctg gagatgcccg agatggctgc cgacacgcgc ttgccgctca cggtcgaagc
     1681 gcgcaagctg gcagactagg ccgatgacat cccagtcgat cgagcgcctt gcggcgacac
     1741 tggtcgccgg caagcttgcg ccctatctcg gccccgaact cctgcttctg gaagcagcca
     1801 atgcctggtt gccggttcgc ccagagacgc ttgccgaacg actggcgaaa cgctcggctg
     1861 ttcccggacg cttgcgccgc aacctcaccg ccgctgcgca gtacatcgag aattttcggc
     1921 accggcgggt gttgcgtggc ctggtccgcg aagtgttcac cgatcgggcg ttgccgaccc
     1981 gcctccacca cctgctcgca gcgctgccac tgccgctcgt ggtcgacgtt ggttacgacg
     2041 cggcgatggc cgcggcgctc gccgagcatc gcgacccgag cgagtggggg caggtgcagg
     2101 cagtgagccg tgccgacgag aaggccgatg tctggacaca gcactacgcg gccggcggcg
     2161 agcgggtcga tccgcgcgcc ggccgcggct ggcgaacgtt gctttataag cccttcggct
     2221 cgatcctgcc tgaggccaac ttccttgtct cggattccga cttcgtcgag gtgctgaccg
     2281 agatcgacat ccagacgccg attcccgaga acgttcagct gcggcgtagt tcgctcggtt
     2341 ttctctttgt cgggtgtcgc ttcgacaacc aactggcaag gacctatgcg cgccaggtca
     2401 gcaagcgatc gagcggtccg cacttcgccg taatgcaggg cgaattgacc gccaacgagg
     2461 cgcgtttcct ggccgagcag ggcatccagc gaattgatat ggcgaccgcc gatttcgtcc
     2521 tgcagctcgg agcggcgatc gagaaggctt tgatcgccgg tgcgaagcgc aatcaggagg
     2581 cggtagcgcc tgtggcgatg tagtcggcga tccgtttgtc ttcgcgcgtg gtgtgcagcg
     2641 cgaaccaggt gaacaggaat tcgacggcct gctcggcagt cccgtcggcg ctgcggaagc
     2701 ggaacatgca gtcgcgaagc tcggcgagca gcgccagatg cagacgacga tgcgcgtcca
     2761 gttcgggata cgcgatatcc gccatcacgt tctcttcgct gacgaagtgg aaatccgcgt
     2821 acttgtagat ctccaacagc agacgggtag cgcgtgtccg atcgccctcc gggctcgcct
     2881 cgttcgaaag gtcgcggatc aggccaagaa atattcgatg ctcgtcgtcg atgcgctcat
     2941 gcccgatttc aaacttgctg tcccagttca gcgtgatcgc catggtttcc ttttcgactg
     3001 gcatccgttg cgcatggaag atcggccacc gggccctgcg cattgatgcg aattcaaagt
     3061 agggcttgag cttaccgggc acagcaacat cgcggggtag cgcctatttg ccatggatca
     3121 agtttgtgtc atttcgatca ctgattcggg tcggctgccg ttcatcgcaa ccagcaagtt
     3181 cgcctggttg tcgaccgatg cccggacgtc cgggtcgagc ttcttcagcc agcgcgccgg
     3241 aatcgccccg gcgccatagg cggccccggc caaccagcca gcaatggcgc cattggtgtc
     3301 ggcatcgccg ccccatttca ccgcgcgcag caggcaggcc tcgaagtcgc cagtcgtgaa
     3361 gaaggcatcg agtgtcgtct gcacggtatc gacgacgtag ccgctcgacc ggcgcgggta
     3421 gggatcgaag cgaaagcagc ggtggctggc gacgagtgtg tcggcatggt cgcgaatgac
     3481 ctccggcgct gcaccggcca gcaggtcggc agtcaggtgc gcgaaggcga ccacggccgc
     3541 gtcggacaac ggatggtgat gcgtcaggtg tgcctgctgc agcgaccact cgacgcgcct
     3601 ctgcgggtca tcgtgggcga gcaggatgac cggcaggttg cgcatcaatg caccgttgcc
     3661 accatcgccc tcgcacgcct ccttgcttag cgacccgtcg atcagataac gctggatgcc
     3721 gcggcgacag gtattgccga catcaatggg ccggctgcgc aaccaagcgg cccaggcatc
     3781 ggcaatggcc ttcaggtcga aagcgccggc attgatgatt gcctcgccca tcgccagcga
     3841 catctgcgtg tcgtcggtga cctggccggg tttcagcctg agccagccgc caccgatgat
     3901 ctcgcggtgt acgccgtact ggtgacggat ttcgccggac gtcatgaact cgaccgtggc
     3961 cccgagtgca tcgccgatag cgagtcccag gtaggcgccc cgcgcgcggt ccagtagcgt
     4021 tagtgcggcg gcgggcttca cagtgtcgcc accttgacct gatagtcgcc gccgaggacg
     4081 aggaactcac cctcgccctt gagcgtcgag ccgggcagca gcgaattggc gaagacgatt
     4141 ttggcaatcg gcacgtcgac ttcgaggatc gaatcgccga actggtcggc gatgtcccgg
     4201 cggtcggtga aagaaaccag gttgttctgg cgggcgatcg ccaggcgctt gcccagccac
     4261 tccacaacct ggtgctcggc caggtcgttg acgccgcggt agagacggag atgccgcgcg
     4321 ccgggcagca gccagcgggc gaacatccac tggctgtact cgtagaggag gtcgagctgc
     4381 gcgttgatgc tgttgttggt aaagcgcgca ctcatctttt cctcgagata agtcatccaa
     4441 ccggcagatg ggtagtggcg cagtggtgcc ttgtggaagg tcggcagcag tccgaagcgg
     4501 ctttcgaccc agcctttcat gacggcgccc tcgcggccgt tggcgtcgaa accccagccg
     4561 cgcagcagcc tcaggtagga ggcgcggtag cgccgccgcc cttggacatc gcggctcgcc
     4621 gggaggtcga gctcgaatac cgtgcgcatg tagatctgga aggcgagcat cgactcctcg
     4681 atgttggttg cctccaagag catcgcgaac agcgtccggt tcgtctcgcg cacgccggag
     4741 atgtgaacat ccggcggttc ggcattgaac gccggactgg ccagcagcgc cgtcgggatg
     4801 ccgatcagat tgctcgtgtg ccagcgctgt gcctcgccga cgtctttgct cggcggtggc
     4861 gtcaacgcaa cgccgcgatc agtaattgct gaaatggcct tcggctttcg cgcgccggcg
     4921 agccttgccg atcagttcct tggtggtgcc gggggccgcc ggcttggcca tcagcttctg
     4981 cagttgcgga tggccgcact gcggacaggc gggctcggcg tcgtggcgaa ccagcatctc
     5041 gctggtgcat tcgcatttgg cgcaatggaa gtcgaacagg ggcattgcgg tctccatagt
     5101 cagggcaaga gtacgcagca agatcggtgc cgaaagtggg catgctcctt gcggtaggtc
     5161 gcgcatgctt aacatcctgc tgattgacga acgacgggaa cgcgccgtgg aaatctgcgg
     5221 tggtctggtc atggctgg
//
LOCUS       DATDAA010000386        11834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_272159,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000386 DATDAA010000000
VERSION     DATDAA010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11834
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272159"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VIS73_11550"
     CDS             <1..183
                     /locus_tag="VIS73_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763831.1"
                     /translation="MNPSQLWETTMDPQVRRLLKVQIEDAIGADEIFSTLMGEPVEPR
                     RAFIESNALRARNIDV"
     gene            complement(266..2890)
                     /locus_tag="VIS73_11555"
     CDS             complement(266..2890)
                     /locus_tag="VIS73_11555"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEY5763832.1"
                     /translation="MTKSLIIAEKPSVAVDIAKALGQVPRQGDFFENDEVIVASAVGH
                     LLELTVPDEYEVKRGKWSFTHLPVLPPHFSLKPIDKTAERLRLLLRLIKRKDVGVLVN
                     ACDAGREGELIFRYLAQYAKTDKPIRRLWLQSMTPASIRDGFAHLRSDGEMQPLADAA
                     RCRSEADWLIGINGTRAMTAFNSAGGGFYKTPVGRVQTPTLAILVEREKRIRAFRSKP
                     FWEVEAEFQAVDGSYRGRWIDLGFRREDRDDEHARAERIWVEGDAVAVQARCQGKPAT
                     ATDESKPTTQLSPLLFDLTSLQREANARFGFSAKNTLGLAQALYEKHKVLTYPRTDSR
                     YLPEDYIATVKETLGVLGSTAYARFANEVAKSGWVHPNKRIFNNAKVSDHFAIIPTAL
                     APKQLSEPEQKLYDLVVKRFLAVFYPAAEHLVTTRITRIGDDAFKTDGKILVAAGWLT
                     VYGKGEQDDDAQLVKLGPDERVWANEVTVKANETKPPARFTEATLLSAMEGAGKLIDD
                     EELRAAMSAKGLGTPATRAATIEGLIYEEYVHREGRELVPTAKADSLLFALEKLGVDE
                     VRSPELTGHWEYQLKQMEAGSLQRDAFMEHIVATTSELVDRIKNGQIPDEAFRTLEAP
                     CPKCGGVVQENYRKFLCKACDFSLWRVVASRQLEPEEVSTLLQNRSVGPLQGFRSKMG
                     RPFAAILKLNAENGLEFDFGQNRDDEEGAEAPDFSGQEPLGACPKCQSKVYEHGLSYV
                     CEQAVGPDRKCDFRSGKIILQQTVETDEMRKLLRDGRTSLLKNFVSQRTRRKFSAYLV
                     RSPEGKVVFEFEKREPRKTAGGAAKKPSGEAAEAAAPKKAAAKAKGKSPTKAESITAK
                     RAAASKTTAKARKTKSSS"
     gene            complement(2980..3435)
                     /locus_tag="VIS73_11560"
     CDS             complement(2980..3435)
                     /locus_tag="VIS73_11560"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEY5763833.1"
                     /translation="MIDILIYLLHSYPAFAAQPLPDKLVHQLKAAGFESDEIETALEW
                     LGTIAQADSADQSYPAPRLSGIRVFAAPEVSALGVEGLSLLLRLEQTGHLDLETRELI
                     IDRVMSFGEAPVSARRLRVVTLAVLWRRSPSMDPLLVEELLTDSTSRLH"
     gene            complement(4526..5623)
                     /locus_tag="VIS73_11565"
     CDS             complement(4526..5623)
                     /locus_tag="VIS73_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEY5763834.1"
                     /translation="MHRIIAALSGALLALTPGFASAQSAAAETVVETRGNVLQLAPDA
                     PDRYVVVKGDTLWGISSRYLLSPWNWPELWGMNLEEVRNPHLIYPGDVLVLDRQAKRL
                     YLERNGKLVDTGREPSTDARLSPQVHVVDNRGAIPSIPADALQPFLTTPLIVEANELA
                     GAPRVIGAQENRVVLGSGDRVFVGGLPGTDQLEWHFYRPGDPLRDPDTGEVLGHEAIY
                     LGDGTLERTGDPALLLIKRAKLEIGQGDRLITAKRALVTNYLPRRPPSGFSGKVAAIH
                     GKGTIGGQYSVITLNRGRQSGLEIGHVVALERAGRIVGVRSEDRGFEQVELPSDRYAL
                     AFVFRTFDRIAYALVLHATVQISIGDVARAP"
     gene            5783..6286
                     /gene="def"
                     /locus_tag="VIS73_11570"
     CDS             5783..6286
                     /gene="def"
                     /locus_tag="VIS73_11570"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY5763835.1"
                     /translation="MALLPILRYPDERLRKVAKPVVTVDERIRRLATDMAETMYAAPG
                     IGLAATQVDVHEQLVVIDVSENKNDLHVFVNPRIEASDGTQEGEEGCLSVPGVYDTVT
                     RAEHIRVHYLDLEGKARKLDAEGLLAVCIQHELDHLQGKVFVDYLSRLKQTRIRSKLT
                     KQARITA"
     gene            6351..7283
                     /gene="fmt"
                     /locus_tag="VIS73_11575"
     CDS             6351..7283
                     /gene="fmt"
                     /locus_tag="VIS73_11575"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY5763836.1"
                     /translation="MKIVFAGTPVFAVPSLRALVDAGHEVSLVLTQPDRPAGRGLRLQ
                     ASPVKEAAQAFDLPILQPPTLKDAAIQARLAEEKADAMVVVAYGLILPKVVLDQPRHG
                     CLNVHASLLPRWRGAAPIQRALLAGDSETGVSIMAMDAGLDTGAVYRRRHLPISASDT
                     AATLHDRLASLGAEALIETLANLNAGTATAQAQPVAGVTYAAKIDKAEAQLDWQRSAI
                     EIGRSIRAFDPFPGALTRLDGAPCKIWAARPIDLVDARAAPGEVLAVDGEGITVACGS
                     GRLLLTSLQRAGGRRLATKDFIAGYPLPVGARFG"
     gene            complement(7197..7994)
                     /locus_tag="VIS73_11580"
     CDS             complement(7197..7994)
                     /locus_tag="VIS73_11580"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEY5763837.1"
                     /translation="MARSCRPADRRRLPGTPEVTVWRRASPPRSGFPDTARHRDWHYV
                     NQPIGESPDRVLRGRLHEAIPQLRAWFADPASLYGAYSLVWLLHLVADLHQPLHVSTR
                     RLPGGGHDAGGNEVDVLIDAALLPHRRRDRSPKLHACWDELVAPPWLAGEQLARLRDR
                     PVHEHPRRGGPVDPAAWRAQSFALAVDAYRGLKASGRPTRISADAHRQNQRIAERQVT
                     LAGYRLADLLGAALAAAENSAESGTDRQRIAGDKILRRQPAAASALQ"
     gene            8144..9430
                     /gene="rsmB"
                     /locus_tag="VIS73_11585"
     CDS             8144..9430
                     /gene="rsmB"
                     /locus_tag="VIS73_11585"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEY5763838.1"
                     /translation="MERSSDATLAAALLLAARQIAVVIEGASLQGHEIAAVAENQRGQ
                     VHDLVYGSLRRYGWGDFLIGRLMPRPPEDTLLRGLLLAALYRLETRPDIAHVVVHQAV
                     EAASRFARGRYTRLVNAVLRNAKRREDELRTALAADPVASNWHPAWWLAELKASYPER
                     WSDIVAAGNEIPPMTLRVNRRRAGADEVVHRFAAEGIALDRVGTLGLLLPRPMAVERL
                     PAYADGLWSVQDAGAQRAAELLAPRDGERVLDACAAPGGKTAHLAELADLELLALDND
                     AIRSRRINENLVRLGLRATIKVGDAGLPARWWDGKPFDRILLDAPCTASGVVRRHPDA
                     KWLRRAEDLAGFAAQQQRLLRALWPLLASGGKLLYATCSVFPRENGEQVSRFLAEQGG
                     ARDVTPGGAADRDPGLQLLPCPEHDGFFYALLEKHG"
     gene            9405..10010
                     /locus_tag="VIS73_11590"
     CDS             9405..10010
                     /locus_tag="VIS73_11590"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEY5763839.1"
                     /translation="MRCWKNTADRQQANQRRRGLAGLAAALCLSAQLAIAAGGIEVIE
                     AQLVPGEAAYRLDADFQITLPPEIEDLINRGIAVHFALSFDLIRERKYWFNRTVSEQR
                     REIRLIYHPITRSYRVSTGLLHQSFDTLDAALRSIARVRGWAVAELAAVNPRKVYRAT
                     LRLELDRGLLPRPFQLQVIATDAWTLDSGEYHWDYHRGAEQ"
     gene            10007..>11834
                     /locus_tag="VIS73_11595"
     CDS             10007..>11834
                     /locus_tag="VIS73_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY5763840.1"
                     /translation="MRRLMVVVAALLGLGLHILLTSLAANTALFTRYYPLLVLLNAVL
                     TLALIGLVIRQVRRLWREHRAGVFGSKLKLRLVLMLAGMAVVPGILLYAVSVQFVTRS
                     IESWFDVKVEKALEAGLALGRSALDTLLDDLARKGDAMVIELAGHPDAVRQAMLGRLR
                     EQGGVPVAAIFSPNGQIIATASAGLAEQRLPVPEVSELRQARLGRTLKRIDSSSENAV
                     LRVLVRIAARDLAEEPRILQLSLPVPPALMKNADEVQAVYRDYQELQLGRKGLTQIYA
                     LTLSMTVLLALSAALAIAFYFARRLSAPLSILAEGTQAVAQGDFTPRRAFKSRDELGI
                     LTQSFNQMTRQLDEARRDAERHRQELEAARAYLESILANLSAGVLVFDRRFVLRLRND
                     GAATILAEDFTGLVGEPLEAWARQPGFARAIRDGFAQSPGNAWQGQVELARDAGPSAG
                     MPQFLLLRGSRLPEAGGGGYVVVFDDITRLVAGQKSAAWGEVARRLAHEIKNPLTPIQ
                     LAAERLPRKLADRIDPESLEIVARGVHTIVDQVRAMKDMVDDFRDYARMPQPVLARVD
                     LGDVVRDVLALYEGSRNAVDSKLDPASLFVLGDRTQLRQVIHN"
BASE COUNT         2105 a         3923 c         3723 g         2083 t
ORIGIN      
        1 atgaacccaa gtcagctttg ggagacgacg atggatcccc aagttcggcg tttgctcaag
       61 gtgcagattg aggatgcaat cggtgccgac gagatatttt caacgctgat gggcgaaccg
      121 gtcgaaccgc gcagggcttt catcgaaagc aatgcgttgc gggctcggaa tatcgacgtc
      181 tgattaatcc gacaacaaag cggtagtcaa aaaagagaag gggccatgcg gccccttctt
      241 tgtttcacgt gaaacaacca agccgtcagc ttgagctttt cgttttccga gccttcgctg
      301 tcgtcttgct tgccgcggcc cgctttgccg tgatgctctc cgccttggtc ggggacttgc
      361 cctttgcctt agccgccgcc tttttcggcg cggcggcctc ggcagcttca ccgctcggtt
      421 tcttggcagc ccccccggca gtcttccgtg gctcgcgctt ctcgaactcg aagacgacct
      481 taccctctgg actgcgaacc agatacgctg agaatttgcg gcgcgtccgt tgcgatacga
      541 agttcttcag caggctggtt cggccatcac gcagcagctt gcgcatctcg tcggtctcta
      601 cggtctgctg cagaataatc tttcctgaac ggaagtcgca cttgcggtcc ggaccgacgg
      661 cctgttcgca aacgtaggac agcccgtgtt cgtagacctt gctctggcac tttgggcacg
      721 cgcccagggg ctcctgaccc gaaaaatccg gcgcctcggc gccttcctcg tcgtcgcgat
      781 tttgcccaaa atcgaattca agcccgttct cggcattcag cttgagaatg gccgcgaagg
      841 gcctgcccat cttgctgcga aatccctgca atggtccgac gctgcgattc tgcaacagtg
      901 tgctgacctc ttcgggttcg agctggcgac tggcaacgac ccgccacagc gagaagtcgc
      961 aggccttgca caagaacttg cggtaatttt cctgaaccac tccgccgcac ttggggcagg
     1021 gggcctctag cgtacgaaag gcctcatcgg gaatctggcc gttcttgatg cgatcgacaa
     1081 gttcgctcgt cgtcgccacg atgtgctcca tgaaggcatc ccgctgcagg ctgccggctt
     1141 ccatctgttt caactggtat tcccagtgac cggtcagttc gggcgaacgg acttcatcga
     1201 caccgagctt ctcgagcgcg aagagcagtg aatcagcctt ggcagtcggt accagctcac
     1261 ggccctcgcg atggacgtat tcctcgtaga tcaggccttc gatggtcgcc gcgcgggtcg
     1321 ccggcgtgcc gagacccttg gcgctcattg ccgcacgcaa ctcctcatcg tcaatgagtt
     1381 tcccggcacc ctccatcgcc gacaacagcg ttgcttcggt aaagcgcgcc ggcggcttgg
     1441 tctcattcgc cttgaccgtt acctcgttcg cccaaacgcg ttcgtcggga ccaagcttga
     1501 ccagctgcgc gtcatcgtcc tgttctccct tcccgtacac cgtgagccag ccggcagcaa
     1561 ccagaatctt gccgtcggtc ttgaaggcat cgtcgccgat tcgcgtgatc cgtgtcgtca
     1621 ccagatgctc ggccgccggg tagaagaccg ccaggaagcg tttgactacc aggtcataga
     1681 gcttctgctc gggttcgctc aactgcttgg gcgcaagagc cgttgggata atcgcaaagt
     1741 gatcgctcac cttggcgttg ttgaaaatcc gcttgttggg gtgtacccag ccgcttttcg
     1801 cgacttcgtt ggcaaatcgc gcgtatgccg tcgaacccag aacgccaagc gtctccttca
     1861 ctgtcgcgat gtaatcttcg ggaaggtagc ggctgtcggt tcgcgggtag gtcagaacct
     1921 tgtgcttctc gtacagggct tgggcgaggc ctaatgtatt cttagccgaa aacccgaagc
     1981 gagcgtttgc ttcccgctga aggctggtca gatcgaagag cagcggagag agctgggtgg
     2041 tcggcttgct ctcgtcggtg gccgtagcgg gctttccctg acagcgcgcc tggacggcaa
     2101 ccgcgtcacc ctcgacccag atgcgctcgg ctctggcgtg ttcgtcgtcc cgatcctcac
     2161 ggcgaaagcc gagatcgatc caacggcccc ggtagctgcc atcgaccgcc tggaactcgg
     2221 cttcgacctc ccaaaagggt ttggagcgaa acgcacggat ccgtttctcg cgctcgacca
     2281 gaatcgccag cgtcggggtc tggacccttc caaccggggt cttgtagaag ccacccccag
     2341 cggagttaaa ggccgtcatc gctcgcgtcc cgttgatgcc gatgagccaa tcggcttccg
     2401 aacggcaacg cgcggcgtcg gcaagcggtt gcatctcgcc atccgagcga aggtgcgcaa
     2461 agccgtcacg aatcgacgcc ggggtcatcg actgcaacca cagacgccga atcggtttgt
     2521 cggtcttcgc gtattgggcc aggtagcgaa agatcaactc tccttcacgg ccggcgtcac
     2581 aggcattgac cagcacacca acgtccttgc gcttgatcag gcgaagcagc aagcgcaggc
     2641 gctcagcggt cttgtcgatc ggcttcagtg aaaagtgcgg tggcagaacc ggcaaatggg
     2701 tgaacgacca cttgccgcgt ttgacttcat actcgtcagg aacggtcagt tccagcaggt
     2761 gaccgactgc agaagcgacg atgacctcat cgttctcgaa gaaatcgccc tgccgtggaa
     2821 cctggccgag cgccttggcg atgtctacgg caaccgacgg tttctcggca atgatcaggg
     2881 acttggtcat gtttgattat tcgacacccg aatcgggcgc cgccatgtta gagaacgggg
     2941 ttggcaaccg gcaagcggcg ccggccatca gacagggagt cagtgcagcc gactcgtcga
     3001 atcagtcagc agttcttcga ccagcaatgg gtccatgctc ggactgcgcc gccaaagaac
     3061 cgccagtgtt acaacccgca ggcgccgcgc cgaaaccgga gcctcaccga aactcatcac
     3121 ccggtcaatg atcaactcgc gcgtctcgag gtcgagatgg ccggtctgct cgagccgcag
     3181 cagcaaagag agcccctcta cgccgagcgc ggagacctcc ggtgcggcaa aaacacgaat
     3241 cccggaaagt cgcggcgctg gatacgactg gtcggccgaa tcggcctgcg caatcgttcc
     3301 tagccattcc agtgccgttt cgatctcgtc agactcgaag ccggcagcct ttagctgatg
     3361 cacgagcttg tccggcagtg gttgtgccgc aaaggccgga taactgtgga gaaggtaaat
     3421 caggatgtct atcattgctg cctgtcagtc gacccgctgg aagtatccgc cggcacgcac
     3481 aataacgcgg cccgacaact caagttcaag aaggatcgca agcacggcat cccccgtcaa
     3541 gccgaccgcc gcgcaaatct ggtcgaccga gcacggagtg tgacccatcg ccgcgagaac
     3601 cggttctccc catcgccgca cgaaacaccc gctcccgcga gcggcaaagg tgtcgcccgc
     3661 ggaccccgtt ccagttcgtt ggtgatgtcc tcaaccgtct caacgagttt cgagccgtcg
     3721 cgaatcagcc gatcgcagcc ttgggattca cgatcaagtt cgccggcaag ccgggcggta
     3781 atcttagagc cactatcaac cgcagtttcg accaccagaa cccaaccgcc aagcgtcgaa
     3841 atcgggcggt tgcggcgcgc aaaattagcc gccagtggcg gaacgctggg tggaaactcg
     3901 gtcagcagtg aaccgcaacc agcgacttga tgagccagat ccagctgccg tgacgggtaa
     3961 atgagatccg agcctgttcc gagtcccgca atcgtggatc ccgatccgcg caagccaccg
     4021 cagtttgcgg caccgtctat tccgagtgcc atgccgctga cgacccatac cccggagcaa
     4081 ctcggggttt gggcgaaccg ctcggcgcta cgttcgccac cggtgctcgc cgcacgacgc
     4141 ccaacaagat agccagcgac ggccgcgtca caagcgaggg ctcgccgaca aaaaagacga
     4201 ttcagcgtgg gtcgctcaga tccagcagcc gacggaggaa ggcttcgtcc gaacaaagca
     4261 cgccacgccg ttcgccacca tctagccgag cgagcgcctc gttcagtctc gtttcgtttg
     4321 ctgccgcatc gaaagactcg gcctgagcgc cgatcaggcg gcgccacgca cccgggccgg
     4381 cggacaccgc gccggatgga ctgccgaggc cttaagcacc accgttggcc gcccggtccg
     4441 atccctggga gcatgaccac ccgtagccaa gccgcccgct catccatcac cgcgacgggg
     4501 cactcgcttg cgaggcgggg gcctctcagg gtgcccgggc gacgtcgccg atgctgattt
     4561 gtaccgtcgc gtggagtacc agggcgtagg cgatccggtc gaaggtacgg aacacgaagg
     4621 ccagcgcgta gcggtcactg ggcagctcga cctgctcgaa gccccggtcc tcgctgcgca
     4681 cgccgacaat ccgcccggcg cgctcaagtg cgaccacgtg gccgatctca agtccgcttt
     4741 ggcgaccgcg attgagcgta atcaccgagt actggccacc aatggtgcct ttgccatgga
     4801 tcgccgcaac cttgccggaa aacccggaag gcggacgccg cggcagataa ttggtgacca
     4861 gcgcacgttt ggcggtgatc aggcggtcgc cctgaccgat ctcgagcttg gcccgcttga
     4921 tcagcaatag cgccggatcg cctgtccgtt cgagcgtacc gtcaccgaga tagatcgcct
     4981 cgtgacctag cacctcgccg gtatcggggt cgcgcagcgg gtcgcccggc cgataaaagt
     5041 gccattcgag ttgatcggtc cccggcagac caccgacgaa cacgcgatcc ccagagccga
     5101 ggaccacgcg gttctcctgg gcgccgatga cgcgcggtgc cccggcgagt tcgttggcct
     5161 cgacgatcag tggcgtagtc aggaacggtt gcagcgcgtc ggcgggaatc gatggaattg
     5221 cgccccggtt gtcgacaacg tggacctgcg gggaaagacg ggcatccgtt gacggctcac
     5281 gcccggtatc gaccagtttg ccgttgcgtt ctagataaag gcgcttggcc tggcgatcga
     5341 gtaccagcac gtcgccgggg taaatcagat gcggattgcg gacttcttcg aggttcatgc
     5401 cccagagctc gggccagttc cacggcgaga gcaggtagcg gctgctgatg ccccagagcg
     5461 tgtcgccctt gacgacgacg tagcgatccg gcgcatcggg cgccagttgc agcacgttgc
     5521 cgcgagtttc gacaaccgtt tcggccgccg cggactgtgc cgaggcaaat cccggtgtca
     5581 gcgcgagaag cgcgccacta agcgcagcta taattcgatg catcttcgta cctccgaccg
     5641 gaaggaacgc tgaaccggca agtccggtcc aattcctgaa cctgcccgcc acgatgccaa
     5701 atcactaatg ttagccggca ccgacttgcg gcagcttctt agattctgca cgtaatcctt
     5761 taactgttca agaattttta caatggcact actcccgatt ttgcgttatc ccgacgagcg
     5821 gctgcgaaag gtcgccaagc ccgtggtgac cgtcgacgag cgaatccgtc gcctggcaac
     5881 cgacatggcc gagaccatgt acgccgcacc cggcattggc ctcgccgcta cacaggtcga
     5941 cgtgcacgag cagctggtcg tcattgacgt ctccgaaaac aagaacgacc tgcacgtgtt
     6001 cgtcaatccg cgcatcgaag ccagcgatgg cacgcaggag ggcgaggagg gctgcctttc
     6061 ggtgccgggc gtctacgaca ccgttacccg ggccgaacac atccgcgtcc attacctgga
     6121 tctggaagga aaagcgcgca agctcgacgc cgaaggcctg ctcgccgtct gcattcagca
     6181 cgagctcgat catctccagg gcaaggtttt cgtcgactac ctgtcgcggc tcaagcagac
     6241 gcgcatccgc agcaagctaa ccaagcaggc gcgcattacc gcctgagccg aatcccgccg
     6301 gcggcgcatt ggcgtccgcg cgccggttac ccaatggaag tcgctcccag atgaaaatcg
     6361 ttttcgcggg aacacccgtg ttcgccgttc ccagcctgcg cgcactggtc gatgccggcc
     6421 acgaggtgtc gctggtcctc acccagcccg accggccggc ggggcgtggt ctcagactgc
     6481 aggcgtcgcc ggtcaaggaa gcggcgcagg cattcgattt gcccatcctg cagccaccga
     6541 cgctgaagga tgccgcgatc caggcgcgcc tcgccgaaga gaaggccgac gccatggtgg
     6601 tcgttgccta cggcctgatc ctgcccaagg tcgtactcga ccaaccccgg cacggttgcc
     6661 tcaatgttca cgcctcgctg ctgccgcgct ggcggggtgc cgcgccgatc cagcgcgcgc
     6721 tgctcgcggg cgacagcgaa accggggtgt cgatcatggc catggacgcc ggcctcgaca
     6781 ccggcgccgt ctaccggagg cgccacttgc cgatcagtgc aagcgatacc gcggcgacgc
     6841 tgcatgatcg cctcgcaagc ttgggcgccg aggccctcat cgaaacgctc gcaaacctga
     6901 acgctggaac cgcgaccgcg caggcgcagc cggtggcggg cgtgacctac gcggcgaaga
     6961 tcgacaaggc ggaggcccaa ctcgactggc agcgttccgc gatcgaaatc ggccgttcga
     7021 tccgtgcctt cgatccgttt cccggcgcac tgacgcgcct tgacggcgcg ccctgcaaga
     7081 tctgggcagc aaggccaatc gacctcgttg acgcaagggc cgcacccggc gaagtactgg
     7141 cagtcgatgg cgaggggatc accgtcgcct gcggcagcgg caggctgctg ctcacctcac
     7201 tgcagcgcgc tggcggccgc cggctggcga cgaaggattt tatcgccggc tatccgctgc
     7261 cggtcggtgc ccgattcggc tgagttttct gccgccgcga gcgcggcgcc gagcagatcg
     7321 gccagccgat agccggccag cgtcacctgc cgctcggcaa tccgctggtt ctggcgatgg
     7381 gcgtccgcgc tgatgcgggt cggcctgccg ctggccttta ggccgcggta ggcatcgaca
     7441 gcgagcgcga agctctgcgc ccgccaggct gcaggatcga ccggcccgcc gcgccgagga
     7501 tgttcgtgaa cgggacgatc gcgcagccgg gcgagttgct cgccggccag ccacggcggt
     7561 gcgacgagct cgtcccagca ggcgtgaagc ttcggcgacc gatcgcgcct gcggtggggt
     7621 agcagcgcgg cgtcgatcag cacatcgacc tcgttgccgc ccgcgtcatg gccgccacct
     7681 ggaagccggc gagtcgaaac atgcaggggc tggtgcaggt cggcaaccag atgtagcagc
     7741 cagaccagcg aataggcccc gtacagcgag gccggatcgg cgaaccaagc ccgcagctgc
     7801 gggatcgctt cgtgcaggcg gccgcgcagc acccggtcgg gcgactcacc gatcggctgg
     7861 ttcacgtagt gccagtcgcg gtgccgcgcc gtgtcgggaa aacccgagcg cggcggcgat
     7921 gctcgccgcc agacggtgac ctccggcgtt ccaggcagcc gccggcgatc cgcaggacga
     7981 caggagcgcg ccaatcgcca gccggatcag ccagcgcacc gcccacaacc cgtttcgctc
     8041 aagcaccatc gaggcattat gcccagccac tgcgctgctt gcggcgatcg ccccggcatg
     8101 gcaccgtgcc ggtccgactt cccgaataag ccatcaatat tccatggagc gatcaagcga
     8161 cgcgacgctc gcggctgcac ttctgctcgc ggcccggcag attgccgtgg tcatcgaggg
     8221 tgccagcctg cagggccacg agatcgccgc cgtcgccgag aaccagcgcg gccaggtgca
     8281 cgacctcgtc tacggcagcc tgcgccgcta cggctggggt gatttcctga tcggtcggct
     8341 gatgccgcgc ccacccgagg acaccctgct gcgcggcttg ctgctcgcag cgctctaccg
     8401 tttggaaacc cggcccgaca tcgcgcatgt cgtggtccat caggcggtcg aagccgccag
     8461 ccgcttcgcg cgcggccgct acacgcgact ggtcaacgca gtgctgcgca acgccaagcg
     8521 acgagaagac gagctacgca cggcgctggc ggcggatccg gtggcgtcga actggcatcc
     8581 ggcctggtgg ctggccgaac tgaaggcgtc ctatcccgag cgctggtcgg acatcgttgc
     8641 cgccggcaac gagattccgc caatgacctt gcgcgttaat cgccgccgcg ccggtgccga
     8701 cgaggtggtg caccggttcg ccgccgaggg aatcgcgctt gatcgggtcg gcacactcgg
     8761 cctgctcctg ccgagaccaa tggcggtcga gcgactgccg gcctacgccg acggcctgtg
     8821 gtcggtgcag gatgccggcg cgcagcgggc cgccgaactg ctagcgccgc gcgacggtga
     8881 acgagtcctc gacgcctgtg ccgcccccgg cggcaagacc gcgcacctag cggaactcgc
     8941 tgatctcgaa ttgctggcgc tcgacaacga cgccattcgc agtcgccgga tcaacgagaa
     9001 tctcgttcgt ctcggcctgc gggcgacgat caaggttggc gacgccgggc tgccggcgcg
     9061 ctggtgggat ggcaagccct ttgaccgcat cctgctcgac gcaccgtgta cggcttcggg
     9121 cgtggttcgt cgccaccccg acgccaaatg gctgcgtcgt gccgaggatc tcgccgggtt
     9181 cgccgcccag cagcaacgcc tgctccgagc gttgtggccg ctgctggcga gcggcggtaa
     9241 actcctctac gcgacctgtt ctgtctttcc gcgagaaaac ggcgagcagg tctcgcgctt
     9301 tctcgccgag caggggggcg ctcgggacgt aacgccaggc ggcgctgcgg atcgcgaccc
     9361 cggcctgcaa ctacttccct gtccggagca cgacggcttc ttctatgcgc tgctggaaaa
     9421 acacggctga ccggcagcag gcgaatcagc gccgacgagg tctcgccggc cttgccgccg
     9481 cgctctgcct gagtgcccag ctcgctattg cggccggtgg catcgaagtc atcgaggcac
     9541 agctggttcc gggcgaagcg gcctaccggc tcgacgcgga tttccagatc acgctgccgc
     9601 cggagatcga ggacctgatc aatcgtggca tcgccgtgca ctttgcgttg agcttcgacc
     9661 tgatccgcga gcgcaagtac tggttcaacc gcacggtatc cgaacagcgc cgggaaatcc
     9721 gtctgatcta tcacccgatc acccgtagct accgcgtgtc gacaggcctg ctgcaccaga
     9781 gcttcgatac actcgatgcc gcgttgcgca gcatcgcccg ggtccgcggc tgggccgtcg
     9841 ccgagctggc tgcggtcaac ccgcgcaagg tctaccgtgc cacgctgcga ctcgagcttg
     9901 atcgcggcct gctgccccgg ccctttcagt tgcaggtgat cgccaccgat gcgtggacgc
     9961 ttgacagcgg cgagtatcac tgggactacc atcgtggagc cgaacaatga ggcggctgat
    10021 ggtcgtcgtc gcggcgctgc tcggcctcgg cctacatatc ctgctgacct cgctggccgc
    10081 caacacggcg ctgtttacgc gctattaccc gctgctggta ctgctcaacg cggttctcac
    10141 gctggcgctg atcgggctgg tcatccgcca ggtccgccgc ctgtggcgcg agcaccgcgc
    10201 cggagtcttc ggctccaagc tcaagctgcg gctggtgctg atgctggctg gcatggcggt
    10261 ggtgccgggc atcctgctct atgcggtgtc ggtgcagttc gtcacgcgca gcatcgaaag
    10321 ctggttcgac gtcaaggtcg agaaggcgct ggaagccggt ctggcgctcg gccgcagcgc
    10381 tctcgacacg ctgcttgacg atctggcgcg caagggcgat gcgatggtga tcgaactggc
    10441 aggacaccca gacgcggtgc gtcaggcgat gctcggccgc ctgcgcgagc agggcggcgt
    10501 gcctgtcgca gcgatcttct caccgaacgg acagatcatc gccaccgcca gcgccgggct
    10561 cgccgagcag cgcctgccgg tgcccgaggt cagcgagctg cgtcaggcgc gactcgggcg
    10621 cacgctcaag cgcatcgaca gttccagcga gaacgccgtg ctgcgcgtgc tggtccgcat
    10681 cgccgcgcgt gatctcgccg aagagccgcg aatcctgcag ttgagtctgc cggtgccgcc
    10741 agcactgatg aagaacgccg acgaagttca agccgtctat cgcgactatc aggagctgca
    10801 actcggccgt aaagggctga cgcagatcta cgcgctgacg ctttcgatga cggtattgct
    10861 cgcgctcagc gcggcgctgg cgatcgcctt ctacttcgca cggcggctgt cggcgccgct
    10921 gtccattctc gccgagggta cgcaggccgt tgcgcagggc gatttcacgc cgcggcgggc
    10981 gttcaagagc cgcgacgaac tcggtatcct cacccagtcc ttcaaccaga tgacgcgcca
    11041 gctcgacgag gcgcggcgcg acgccgagcg gcaccggcag gagctggagg cggcgcgcgc
    11101 ctacctcgaa agcatcctcg ccaatctctc ggccggggtg ctggtcttcg atcggcgctt
    11161 cgtcctgcgc ctgcgcaatg acggcgccgc gacgattctt gcagaggact tcaccggtct
    11221 ggtcggcgaa ccgctcgagg cgtgggcgcg gcagccggga tttgcccgtg ccattcgcga
    11281 cggcttcgcc cagtcgcccg gcaacgcctg gcagggacag gtcgaactgg cgcgcgatgc
    11341 cggaccgtcg gcgggcatgc cgcagttcct actgttgcgt ggctcgcgtc tgcccgaggc
    11401 cggcggcggc ggctacgtcg tcgtcttcga cgacatcacg cgactggtcg ctggccagaa
    11461 gtcggcggcc tggggcgaag tggcgcgacg cctggcccac gaaattaaga acccgctgac
    11521 gccgatccag cttgccgccg aacgtctgcc gcgcaagctc gccgatcgca tcgatcccga
    11581 gtcgctggag atcgtcgccc gcggcgtgca caccatcgtc gaccaggtgc gggcgatgaa
    11641 ggatatggtc gacgacttcc gcgattacgc cagaatgccg cagccggtgc tggcgcgcgt
    11701 cgatttgggt gacgtcgtgc gcgatgtcct tgcgctctac gagggcagcc gcaacgcagt
    11761 cgacagcaag ctcgatccgg cttcgctgtt cgtgctcggc gaccgcacgc aactgcggca
    11821 ggtgatccac aacc
//
LOCUS       DATDAA010000387         2432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_483080,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000387 DATDAA010000000
VERSION     DATDAA010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483080"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VIS73_11600"
     CDS             <1..475
                     /locus_tag="VIS73_11600"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HEY5763841.1"
                     /translation="PPSFIHHPASTLPHIEIHGAKLTVIVGEAFGHRSPVATLWPTLY
                     VDALLPAGASLEVPAEHEQRAVYVVDGAVRVTETAVTLAQMAVLESGRSVTLQALTAS
                     RVMLLGGARFKTPRYIWWNFVSSSPERIERAKQDWRTNRFPEVPGETEFIPLPER"
     gene            complement(662..>2432)
                     /locus_tag="VIS73_11605"
     CDS             complement(662..>2432)
                     /locus_tag="VIS73_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylosucrase"
                     /protein_id="HEY5763842.1"
                     /translation="EMLATNYIRRSDSLKAVDLQRERDHNWFLSQQWVGMALYCHRFA
                     GDLKGLKANLGYLQELGINLLHIMPMMDCPRGKNDGGYAVRDFRALDPRSGTLEDLDA
                     LIRNMDKRGMLIALDVVVNHTSEEHEWARRACAGETAYQDYYYTFVDREYPDLYEESM
                     PEIFPETAPGNFTWNEEMQRWVMTVFNNYQWDLNYRNPAVFIEMMDIILFWANRGVDV
                     LRLDAVAFLWKRLGSSCQNEREAHLILQLFKDCCQVVAPGVLFIAEAIVAPVEVIKYF
                     GEDAVIAKECEIAYNATYMALLWDAVATQRADLLNIGIRNLPDKLERATWLNYVRCHD
                     DIGWGFADEDIRQAGYEPRAHRSFLVDYYTGQFEGSPARGSAFAFNKRTGDARISGSL
                     ASLAGLESAIEAGDAEATAMAIRTIVMLHALIMSFGGIPLLFYGDAIGTINDASYLSD
                     HEKHDDNRWMHRPNFDWEKAKLRQQPGSVEHRIFTALRHLIAVRKETAAFADLDNREL
                     LAVENPHLFVFARYDPMRTGPRILVVANFSLATQALPLAGLRAKGFLTRDHAKDIRSG
                     DIVPLAEDALAVGGREFFWLREA"
BASE COUNT          432 a          757 c          785 g          458 t
ORIGIN      
        1 cccaccgtcg ttcatacatc accccgcgtc cacgcttccg cacatcgaaa tccatggcgc
       61 caagctgacc gtcatcgtcg gcgaagcgtt tggtcaccgc tcgccggtgg cgaccttgtg
      121 gccgaccctg tacgtcgacg ccctacttcc ggcaggtgct tcgctcgaag ttccggccga
      181 gcacgaacag cgcgcggtct atgtcgtcga tggcgcggtg cgtgtgaccg agacagcggt
      241 tacgctcgca cagatggcgg tgctcgaaag cggcagaagc gtcacgctgc aagcgctgac
      301 cgcttcaaga gtcatgttgc tgggcggtgc gcgtttcaag acaccgcgct atatctggtg
      361 gaattttgtc tcgtcttcgc ccgaacgtat cgaacgggcg aagcaagact ggcgcaccaa
      421 tcgattccca gaagtaccgg gcgagaccga gttcataccc ctgcccgaac gctgaatcgt
      481 cgggcagctt gccaccgagc cacggacacg cgactggcag cgttcgcacc agcaccgccg
      541 atcgtcgatc gccgcgcgat ggagcccgat tcggcgctaa tcgtcgagcg tgtggattac
      601 ctacaagcgg ttctttaaca gccgaccgag aactgacgcg gggatcgccg tcgccttacg
      661 cttacgcttc gcgcagccag aagaactctc ggccgccgac cgccagcgcg tcttcggcaa
      721 gcgggacgat gtcgccgctg cgaatgtcct tggcgtgatc tcgggtcaga aaccccttgg
      781 cgcgcaggcc ggccaacggc agcgcctggg tggcgagcga gaagttggcg accactagga
      841 tgcgcgggcc ggtgcgcatc gggtcgtagc gcgcgaagac gaacaggtgc ggattttcga
      901 cggccagcag ttcgcggttg tctaagtcgg cgaaggccgc cgtctccttg cgcacggcga
      961 tcaggtggcg cagtgcggtg aagatacggt gctcgacgct gcccggctgc tgacgaagtt
     1021 tggccttctc ccagtcgaag ttcggacggt gcatccagcg gttgtcgtcg tgcttttcgt
     1081 ggtcgctgag gtaggacgcg tcgttgatcg tgccgatcgc gtcgccgtag aacagcaatg
     1141 gaatgccgcc gaaggacatg atcagcgcgt gcagcatcac gatggtccgg atcgccatcg
     1201 ccgtcgcctc cgcatcaccg gcctcgatcg ccgattcgag gccggcgagc gaagccagcg
     1261 aaccggagat tcgggcatcg ccggtccgct tgttaaaggc aaaggcgctg ccgcgcgccg
     1321 gcgacccttc gaactggccg gtgtagtagt cgaccaggaa gctgcggtgc gcgcgcggct
     1381 cgtagccggc ctggcggatg tcttcgtcgg caaagcccca gccgatgtcg tcgtggcagc
     1441 gcacgtagtt gagccaggta gcgcgttcca acttgtctgg aaggttgcga atgccgatgt
     1501 tcaggaggtc ggcgcgctgc gtcgccaccg catcccacag cagcgccatg taggtggcgt
     1561 tgtaggcgat ctcgcattcc ttggcaataa ccgcgtcttc gccgaagtac ttgatcacct
     1621 cgaccggggc gacgatcgcc tcggcgatga acagcacgcc cggcgcaacg acctgacagc
     1681 agtccttgaa cagctgcagg atcaggtgcg cctcgcgctc gttctggcag ctgctgccga
     1741 gccgcttcca gaggaaagcg acggcgtcga gccgcaacac gtcgacgccg cgattggccc
     1801 agaacaggat gatgtccatc atctcgatga acaccgccgg gttgcggtag ttcaggtccc
     1861 actggtagtt gttgaaaacc gtcatcaccc agcgctgcat ctcctcgttc caggtgaagt
     1921 tgcccggcgc ggtttccgga aagatctcgg gcatcgactc ctcgtagaga tccggatact
     1981 cgcggtccac aaaggtgtag tagtagtcct ggtacgcggt ctcgccggca caggcgcggc
     2041 gcgcccactc gtgttcttcg gaggtgtggt tcaccaccac gtcaagcgcg attagcatgc
     2101 cgcgcttgtc catgttgcgg atcagtgcgt cgaggtcttc aagtgtgccg ctgcgcgggt
     2161 cgagggcgcg aaagtcgcga accgcgtagc cgccgtcgtt cttgccgcgc gggcagtcca
     2221 tcatcggcat gatgtgcagg aggttgatgc cgagctcctg caggtagccc aagttagcct
     2281 tgagcccttt gagatcgccg gcgaagcgat ggcagtacag cgccatgccg acccactgct
     2341 ggctcaggaa ccagttgtgg tcgcgctcgc gttggagatc gactgccttg agcgaatcgg
     2401 acctgcggat gtagttggtc gccagcatct cg
//
LOCUS       DATDAA010000388         4349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_483276,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000388 DATDAA010000000
VERSION     DATDAA010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483276"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VIS73_11610"
     CDS             complement(<1..343)
                     /locus_tag="VIS73_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763843.1"
                     /translation="MTLIGPPIIAMPEYSALLAGSGSPQSIAIKRHGTRLASRNVAKW
                     PGSSVRAWRMIATGFNGGIRWSAWRHFTVAGALAMAMSGALAADVTLIGTSASRAVFR
                     INGVTKIIRPGQ"
     gene            333..878
                     /locus_tag="VIS73_11615"
     CDS             333..878
                     /locus_tag="VIS73_11615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEY5763844.1"
                     /translation="MSVNDPLPWIGKECELIRHAVTSSVPVIGHCLGGQLLSHALGGA
                     VSASPSREIGWRPIRLDDSEQAAQWFGSPGREVTVFQWHGETFSIPDGTTRIASGDDC
                     PNQAFALGPHLAMQFHVEMTAELIRIWCEEWREVFDPTRRRQASIQTADEMLAQLEIH
                     LAPMRTLAERIYGRWLHGLRN"
     gene            complement(879..1364)
                     /locus_tag="VIS73_11620"
     CDS             complement(879..1364)
                     /locus_tag="VIS73_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY5763845.1"
                     /translation="MPSFDIVSEVDIVALKNALDVSGRKIGNRHDFQGTSAAVELNDK
                     TIVIHGDSEFQLDQIERVVLPELTAKKIDIKCLEKGSVQKVSGNKVKLEIKVRVGVEQ
                     ELAKKVVRLLKDSKLKVQAAIQGDAVRVSGAKRDVLQDAIALVRKEVTDFPLQYNNFR
                     D"
     gene            complement(1427..1744)
                     /locus_tag="VIS73_11625"
     CDS             complement(1427..1744)
                     /locus_tag="VIS73_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HEY5763846.1"
                     /translation="MPQFDKVSVVKKANVYFDGKCVSHTVVLADGTRKSVGVIMPASL
                     SFNTGEPEIMEGVAGSCRVRLKGETGWTRYTAGESFAVPGNSSFEIACDEPYHYVCHF
                     ASK"
     gene            complement(1746..1973)
                     /locus_tag="VIS73_11630"
     CDS             complement(1746..1973)
                     /locus_tag="VIS73_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEY5763847.1"
                     /translation="MTATPTLFGFTEAQIAEFGVTFGIGAFILFMLFIIGEIAWRSKA
                     GKTGTIVLFVVLAFGMFGFVAKTVIQKIWGI"
     gene            complement(1986..3269)
                     /locus_tag="VIS73_11635"
     CDS             complement(1986..3269)
                     /locus_tag="VIS73_11635"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY5763848.1"
                     /translation="MSDVKKVVLAYSGGLDTSVILKWLQDSYQCEVVTFTADLGQGEE
                     LEPARSKAIQFGIKPKNIFIDDLREEFVRDFVFPMFRANTVYEGEYLLGTSIARPLIA
                     KRLVEIARKTGADAISHGATGKGNDQVRFELGAYALMPNVKVIAPWREWDLLSREKLM
                     AYADKHGIPVDMKHRKGGSPYSMDANLLHISYEGRHLEDPAAEAEESMWRWTVSPEKA
                     PNKAEYLDLEFKRGDLVAINGKKMKAHELLATLNKIGGKHGVGRLDLVENRYVGMKSR
                     GCYETPGGAILLKAHRAIESVCLDREVAHLKDDLMPRYASLVYNGYWWSPERRALQAL
                     IDQTQDCVNGWVRLKLYKGNVIVTGRDSKTDSLFDPTIATFEDDAGAYDQKDAGGFIK
                     LNALRLRIAENARQQRAAPAKRSAKAAKSERKRKK"
     gene            complement(3322..4254)
                     /gene="argF"
                     /locus_tag="VIS73_11640"
     CDS             complement(3322..4254)
                     /gene="argF"
                     /locus_tag="VIS73_11640"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY5763849.1"
                     /translation="MSAVRHFLQYKDISSEEQDYLFARTKWIKGEFKSFRKYWPLVDR
                     TLVMIFEKASTRTRLSFEAGMQQLGGTAIYLNTRDSQLGRGEPVEDAAQVISRMSDLV
                     MIRTFEQEIIDRFAGSSRVPVINGLTNEYHPCQILADIYTYIEHRGSIRGKTVAWIGD
                     SNNVCNTWLQAAEVFDFNVHVSTPPGYEVEPDRAGLYGTDHFEQFADPMDAARGADLV
                     TTDVWTSMGFEAENEERRVAFADWQVDAEMMRVASPTALFMHCLPAHRGEEVTADVID
                     GPQSVVWDEAENRLHVQKALMEFLLLGRIDTPDA"
BASE COUNT          802 a         1380 c         1305 g          862 t
ORIGIN      
        1 cctgccccgg gcggataatc ttggtgaccc cgttgatgcg aaacaccgcg cggctcgccg
       61 aggtcccgat cagcgtcacg tcggccgcca gcgcgcccga catcgccatc gccagtgctc
      121 cggcgaccgt aaaatgacgc cacgcagacc accggattcc gccgttgaaa cctgttgcaa
      181 tcattcgcca cgcccgcacc gaagagccgg gccattttgc cacattcctc gaggccaacc
      241 gggtgccgtg gcgcttgatc gcgatcgact gcggcgaccc acttccagcg agcagtgcgg
      301 aatactccgg catcgcgatg atgggcggac cgatgagtgt caacgatccg ttgccgtgga
      361 tcgggaagga atgcgaactg attcgtcacg ccgttaccag cagtgttccg gtcattggcc
      421 attgcctcgg cggccaactg ttgagccatg cgcttggcgg ggcggtcagt gcgagcccgt
      481 cgcgcgagat cggctggcgc ccgattcgcc tcgacgacag cgaacaggct gcgcaatggt
      541 tcggatcgcc ggggcgcgag gtcacggttt tccagtggca cggtgaaaca ttttcgattc
      601 cggacggcac gacaagaatc gccagcggcg acgactgccc gaatcaggcc ttcgcgctgg
      661 ggccgcatct ggcgatgcag tttcacgtcg aaatgactgc cgaactgatc cggatctggt
      721 gcgaagagtg gcgcgaggtt ttcgacccga cgcggcggcg gcaggcgtcg attcagacgg
      781 ccgacgagat gctggcgcaa ctcgagatcc atctcgcgcc gatgcggaca ctcgccgagc
      841 gcatctacgg ccgctggctg cacggcctgc ggaactgatc agtcgcggaa attgttgtac
      901 tgcagcggga agtcggtgac ttccttgcgc accagcgcaa tcgcatcctg cagtacgtcg
      961 cgcttcgcgc cggaaacacg cacggcgtcg ccctggatgg ccgcctgaac cttgagcttc
     1021 gagtccttga gcagcctgac gacctttttc gccagttcct gctcgacacc cacgcgcacc
     1081 ttgatctcca gcttcacctt gttgcccgag accttctgga cgctgccctt ttccaggcat
     1141 ttgatatcga tctttttcgc ggtcagttcc ggcaggacaa cgcgctcgat ctggtcaagc
     1201 tgaaattcgg agtcaccgtg gatcacgatg gtcttgtcgt tcagttcgac ggcggccgag
     1261 gtgccctgaa agtcatggcg gttgccaatc ttgcggccgg atacgtcgag cgcgttcttc
     1321 agcgcgacga tatcgacttc ggaaacgatg tcgaaggatg gcatctcatt gctcccggtt
     1381 aactcgggaa agcgggcgtc ccgcgttccc gatggctgcc tgcaacctac ttggatgcga
     1441 aatggcagac gtagtggtaa ggctcgtcgc aggcaatctc gaacgaagag ttccccggca
     1501 cggcaaagga ttcaccggcc gtatagcgtg tccagccggt ttcgcccttg agccgaaccc
     1561 ggcaactgcc tgcgacgcct tccatgatct ccggctcgcc ggtgttgaag ctcagcgacg
     1621 ccggcatgat gacgccaacg ctcttccgcg ttccgtcggc aagcacgacc gtatggctca
     1681 cgcacttgcc atcgaagtag acgttggcct tcttgaccac cgaaaccttg tcgaactgcg
     1741 gcatatcaga ttccccagat tttttggatg acggtcttgg cgacgaaacc gaacatgccg
     1801 aaggccagca cgacgaaaag aacgatggtc ccggtcttgc cggctttcga ccgccaggcg
     1861 atttcaccga tgatgaagag catgaaaagg atgaatgcgc caatcccgaa ggtgacgccg
     1921 aattcagcaa tctgcgcctc ggtgaaaccg aacagcgtcg gcgttgcagt catccctgag
     1981 cgaccttatt tcttgcgctt gcgctccgat ttcgcggctt tcgcagaccg cttcgccggc
     2041 gccgcgcgct gctgccgcgc gttctcggca atgcgcaggc gcagggcatt gagcttgatg
     2101 aaaccaccgg catccttctg gtcgtaggcg cccgcatcat cctcgaaggt ggcaatggtc
     2161 ggatcgaaca gggaatcggt cttcgaatca cgaccggtga cgatcacgtt gcccttgtac
     2221 agcttgagcc gtacccagcc gttgacgcaa tcctgcgtct ggtcgatcag ggcctgcaac
     2281 gcacggcgct ccgggctcca ccaatacccg ttgtagacca ggctcgcgta gcgcggcatc
     2341 aggtcgtcct tcagatgtgc aacctcgcgg tcgaggcaga ccgactcgat tgcccggtgc
     2401 gccttaagca ggatcgcccc gcccggggtc tcgtagcagc cgcgcgactt catgccgacg
     2461 tagcggttct cgacgaggtc gagacggccg acgccgtgct tgccgccgat cttgttcagg
     2521 gtcgccagca gctcgtgcgc cttcatcttc ttaccgttga tggcgacgag atcaccgcgc
     2581 ttgaactcga ggtcgagata ctcggccttg ttcggcgcct tttccggcga cacggtccag
     2641 cgccacatgc tctcctcggc ctcggccgcc ggatcctcga ggtggcgtcc ttcgtaggaa
     2701 atgtgcagca ggttcgcatc catcgagtac ggcgaaccgc ccttgcgatg cttcatgtcg
     2761 accggaatac cgtgcttgtc tgcgtaagcc atcagctttt cgcgcgagag cagatcccat
     2821 tcacgccacg gcgcgatgac cttgacgttg ggcatcaggg cataggcgcc gagttcgaaa
     2881 cggacctgat cgttgccctt gccggtagcg ccatgcgaaa tcgcgtcggc gccggtcttg
     2941 cgggcgattt caacgaggcg cttggcgatc aatgggcggg cgatcgaggt tccgagcaga
     3001 tactcgcctt cgtagacggt gttggcacgg aacatcggaa acacgaaatc gcggacaaac
     3061 tcttcgcgca ggtcatcgat gaaaatgttc ttcggcttga tgccgaactg gatcgccttg
     3121 gagcgcgccg gctcgagctc ttcgccctga cccaggtcgg cggtgaaggt caccacttcg
     3181 cactggtagc tgtcctgcag ccacttgagg attaccgaag tatcgaggcc tcccgagtag
     3241 gccagtacca cttttttgac gtcgctcatc ttgtcttccg ttggatttgc gggcgaccga
     3301 cgccgcccgg agttggcatg actacgcgtc gggcgtatcg attcgcccga gcagcaggaa
     3361 ttccatcagt gccttctgca cgtgcaggcg gttctcggcc tcatcccaga ccaccgactg
     3421 cggcccgtct atcacgtccg ccgttacctc ctcgccgcgg tgggcgggca ggcaatgcat
     3481 gaacaaggca gtcggcgacg caacgcgcat catctcggcg tcgacctgcc agtccgcgaa
     3541 ggcgacgcga cgctcctcgt tctcggcttc gaagcccatc gaagtccaca catctgtcgt
     3601 caccagatcg gcgccacgtg ctgcgtccat cgggtcggca aactgctcga agtggtcggt
     3661 gccgtacagc ccggcacggt ccggctcgac ttcatagccc ggcggcgtcg agacatggac
     3721 attgaaatcg aaaacctcgg cagcctgcag ccaggtattg cagacgttgt tcgagtcacc
     3781 gatccacgcg acggtcttgc cgcgaatcga gccgcgatgc tcgatgtagg tgtagatgtc
     3841 ggcgaggatc tggcaggggt ggtactcgtt ggtcaggccg ttgatgaccg ggacccgcga
     3901 actgcccgca aagcgatcga tgatctcctg ctcgaaggtg cggatcatca ccaggtcgct
     3961 catccgcgag atcacctgcg cggcatcctc gacgggctcg ccgcgaccaa gttgcgagtc
     4021 gcgcgtattc aggtagatgg ccgtgccgcc gagttgctgc atgccggcct cgaaggacag
     4081 gcgggtgcgg gtgctggcct tttcgaagat catcaccagc gtccggtcga ccagcggcca
     4141 gtacttgcgg aacgacttga attcgccctt gatccacttc gtgcgggcga acaggtaatc
     4201 ctgctcctcg ctcgaaatgt ccttgtattg cagaaaatgc cgaacagcgc tcacgacgcc
     4261 cccctcaagc cgacgcgttc agaaacgccc tgaccaactc gccgaggcgc gacaccagtt
     4321 cgtcgacctc gtcatcggtg atcaccagc
//
LOCUS       DATDAA010000389         4286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_308748,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000389 DATDAA010000000
VERSION     DATDAA010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308748"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..403)
                     /locus_tag="VIS73_11645"
     CDS             complement(176..403)
                     /locus_tag="VIS73_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763850.1"
                     /translation="MQFQLEKYGFRIRTRSGGIVDNLSIHAAEPDAAEQKLRRMYPGC
                     QILESWNERARLGPQATSFEEVIDLIAPSHV"
     gene            complement(486..1304)
                     /locus_tag="VIS73_11650"
     CDS             complement(486..1304)
                     /locus_tag="VIS73_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5763851.1"
                     /translation="MRIAAAWRQSGGRLASNWASGWSFIELVEYPWRFGRDSTPPPVQ
                     PIGWKCCSAACLALAMHFASWISSLEYPSVASSTALVTTNPLWIGLASFFLFGERLSR
                     LTIAEIALSLCGSLLIFVSDSQQAGGPDPLLGNGLALLGSWCSSAYLILGRRLRANLS
                     LSLYIWLAYGVAALFLFGAAGLAGAPLIGFGLIGRLALLGMAIGQQLIGHTSYNWSLR
                     HVSPTFVAVVTLGEPVGSAPLAFVLLDESLAGLQSAGFVLLLAEIYLPVRGEGR"
     gene            1231..2589
                     /locus_tag="VIS73_11655"
     CDS             1231..2589
                     /locus_tag="VIS73_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763852.1"
                     /translation="MNDQPLAQLLANLPPDCRQAAAILNRGCACLALDHDRLAAALAE
                     DGGDAVAAMLAERPHLFADSLLFVADRHLEQMAALVAAVEAVVALPAYVERILGHSPD
                     IARFEPRAKGVFLGFDFHEGADGPRLIEINTNAGGGLLAATLIDAQPECCISTAGTGG
                     AALKELAGARAYHAPVVVAAFVDMFRAEWRLERGDAPLHRIAIVDEAPAGQYLAPEFE
                     LFRRLFLRHGIDALIVDPAELALRDGVLVAGELPVDLVYNRLTDFAFDQPAQAVLREA
                     YLGRAAVVTPHPRAHALFADKRNLIVLRDEDWLRGIGVDAATRATLIELIPETREVTP
                     ENAAEFWRDRRRWFFKPFAGYGSRAAYRGEKLTTRVFETIARGGYVAQSLVPPSQRRA
                     TSARGGGDLKVDVRNFAYGGHVQLVSARLYQGQTTNFRTPGGGFASVFAVRCGDTEMV
                     AA"
     gene            2586..3107
                     /gene="mobB"
                     /locus_tag="VIS73_11660"
     CDS             2586..3107
                     /gene="mobB"
                     /locus_tag="VIS73_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEY5763853.1"
                     /translation="MKVFGIAGYSGSGKTTLIEKLIPLLRGHGLSVSVIKHTHHDFDL
                     DRPGKDSWRHRQAGAAEVVLAGEHRWALLHELRETPRPPLESIVARLAPVDLVLVEGF
                     KHEPIAKLEVIRHELGKPTLWPDDPHVVAIASDRPVDTTLPGFGLDAAEEIARFIAAR
                     VSGALPNQSGEES"
     gene            3104..3358
                     /gene="moaD"
                     /locus_tag="VIS73_11665"
     CDS             3104..3358
                     /gene="moaD"
                     /locus_tag="VIS73_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEY5763854.1"
                     /translation="MNVRLLFFASLREAIGVASESLVLPAEVASVGALRGHLAARGGN
                     WQRLAAGGNVRCALDQTMADDAAALREGAEIAFFPPVTGG"
     gene            3360..3830
                     /gene="moaE"
                     /locus_tag="VIS73_11670"
     CDS             3360..3830
                     /gene="moaE"
                     /locus_tag="VIS73_11670"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HEY5763855.1"
                     /translation="MDQTRDSVRVQGADFDAAAELAALRLGRNDIGAIASFVGLVRDS
                     HAGAEIRGMTLEHYPGMTEKALEAIVDEARQRWSLLGVRVIHRVGPLAPGEQIVLVAV
                     ASAHRGEAFDACEFIMDYLKTRAPFWKHEQTPDGGRWVDARETDDTAAARWQRD"
     gene            complement(3941..>4286)
                     /locus_tag="VIS73_11675"
     CDS             complement(3941..>4286)
                     /locus_tag="VIS73_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763856.1"
                     /translation="ADPVVLDSLGRFRLPNEHTDPRARPLAGDPDAALANTSRGALSA
                     FLRLADRHQQILQLVIDRLCRQGQTQNGKEQTQANDKSHGGDRRFRSVAGSARWLGEL
                     RKPTGDTGSFHY"
BASE COUNT          742 a         1382 c         1454 g          708 t
ORIGIN      
        1 gggaggtctg ccagacgatc atcaacgaga acgtctcggt cgccgagagc ccggcccttc
       61 gtcgcgatgt cttttcgcac gcgccgggca gccagggggc caccgactac caggacctct
      121 acgtcgaact gcgggcgaaa ggaatgctgt agcgcgacca cgcgcggcgg gaacctcaga
      181 cgtgggacgg cgcgatgagg tcgatcactt cctcgaagga ggttgcctgc ggtccgagcc
      241 gggcacgctc gttccacgat tcgaggatct ggcaaccggg gtacatgcgg cgcagcttct
      301 gctctgcagc gtcgggttca gcagcgtgaa tcgacaggtt gtcgacgatt cctcccgacc
      361 gggtccgaat ccggaagccg tacttctcaa gctgaaattg catgtcgcac cgcctgcagt
      421 cgaaacgggg tgccccaata tagtcctatg ctcccggtgc ttcaagcagg cggcatgagc
      481 cggcatcaac gcccctcgcc gcgtacaggg aggtagattt cggcaagcag cagcacgaag
      541 ccggccgatt gcagtccggc gagcgactcg tcgagcagca cgaaggccag cggcgcgctg
      601 ccaaccggtt ctcctagcgt aaccacggca acgaaggtcg gcgatacgtg gcgtaacgac
      661 cagttgtagc tggtatggcc gatcagctgc tggccaatcg ccatccccag cagggccagc
      721 cgcccaatca ggccgaagcc gatgagcggt gcgccagcga ggccggcggc gccgaacagg
      781 aacagcgcgg cgacgccgta ggcgagccag atgtacaggg acagcgacag attggcgcgc
      841 aggcggcgac cgaggattag gtaggcagaa gaacaccacg agccaagcag cgccaacccg
      901 ttgccgagca gtgggtcggg tccgccggcc tgttggctgt cggatacgaa gatcagcagc
      961 gagccgcaca gcgacagcgc gatttcggca atcgtcagcc ggctcagccg ttcgccgaac
     1021 aggaagaagc tggcgagacc gatccacagc ggattggtcg tgacgagcgc ggtcgacgag
     1081 gcgaccgagg ggtattcgag cgagctgatc caggaagcga aatgcatggc tagggcgagg
     1141 caagcggcac tgcagcactt ccacccgatc ggctggaccg gcgggggcgt agaatcccgc
     1201 ccgaaacgcc atggatactc gacgagttca atgaacgacc agccactggc ccagttgctc
     1261 gccaacctgc cacccgattg tcgccaggcc gcagcgattc tcaatcgcgg ctgtgcctgc
     1321 ctcgcccttg accacgaccg gctcgccgcc gcactggccg aagacggggg cgacgcggtg
     1381 gcggcaatgc tcgccgagcg gccgcacctg ttcgccgatt cgctgctatt cgtggccgac
     1441 cgtcacctgg aacagatggc ggcgctggtc gccgccgttg aagcggtggt tgcgctgccg
     1501 gcctacgtcg agcggatcct cggccacagc ccggacatcg cccgctttga gccgcgtgcc
     1561 aaaggggtat ttctcggctt cgatttccac gaaggcgccg acgggccgcg gctgatcgag
     1621 atcaacacca atgccggcgg tgggctgctt gcggcgacgc tgatcgacgc ccagcccgag
     1681 tgttgcatca gcacggcagg aacgggcggg gcggcgctga aggagttggc cggtgcgcgg
     1741 gcctaccatg cgcccgtggt ggtcgccgcc ttcgtcgata tgtttcgcgc cgaatggcgc
     1801 ctcgaacgcg gcgatgcgcc gctgcaccgg atcgccatcg tcgacgaagc gccggcgggc
     1861 cagtatctgg cgcccgaatt cgagctcttt cgccgactat ttctgcgcca cggcatcgac
     1921 gcgttgatcg tcgatcccgc cgaactcgcc ttgcgcgacg gcgtgctggt cgccggcgag
     1981 ttgccggtcg acctggtcta caaccggctg accgatttcg ccttcgacca gccggcgcag
     2041 gctgtgctcc gcgaggccta cctcggcagg gccgcggtgg tcacgccgca cccgcgcgcg
     2101 cacgccctgt ttgccgacaa gcgcaatctc atcgtcctgc gtgacgagga ctggctgcgc
     2161 ggcatcggcg ttgacgccgc aacgcgcgcg acgctgatcg aattgattcc cgagacccgc
     2221 gaagtcacac cggaaaacgc ggcggaattc tggcgagacc ggcgccgctg gttcttcaaa
     2281 cccttcgccg gctacggcag ccgcgccgcc taccgtggcg agaagctgac gacgcgtgtc
     2341 ttcgaaacca tcgcccgagg gggctatgtc gcccagtcgc tggtgccgcc ctcgcagcga
     2401 cgcgccacct cggcgcgtgg cggcggcgac ctcaaggtgg acgtgcgcaa ctttgcctac
     2461 ggcgggcacg tgcaactggt ctcggccagg ctctatcagg gccagaccac caatttccgc
     2521 acgcccggcg gtggcttcgc ctcggtcttt gccgtgcgtt gcggtgatac tgaaatggtt
     2581 gccgcatgaa ggtctttggc atcgccggct acagtggcag cggcaagacg acgctgatcg
     2641 agaagctgat tccgctgctg cgcggccacg gtctgtcggt ttcggtgatc aagcacaccc
     2701 accacgattt cgacctcgac cggccgggca aggattcctg gcggcaccgg caggctgggg
     2761 cggccgaggt ggtgctcgct ggcgagcacc gctgggccct gttgcacgag ctgcgcgaaa
     2821 cgccgcgacc gccgctggaa agcatcgttg ccaggctggc accagtcgac ctcgtgctgg
     2881 tcgaaggttt taagcacgag ccgatcgcca agctcgaggt gatccgccac gaactcggca
     2941 agccgacgct ttggccagat gatccacacg tcgtcgcgat cgccagtgac cggccggtcg
     3001 atacgacgct gccgggtttc ggcctcgacg ccgccgaaga aattgctcgt ttcattgcag
     3061 cccgcgtgtc gggcgcgttg ccgaatcagt ccggagagga atcatgaacg ttcgtctgct
     3121 gttctttgcc agcctgcgcg aggccatcgg ggtcgccagt gaatcgctgg tgctgccggc
     3181 cgaagtggcc agcgtcggcg cgctgcgcgg ccatctggcg gcgcgtggcg gcaactggca
     3241 acggctcgcc gccggtggca acgtgcgttg cgcgctcgac cagacgatgg ccgacgacgc
     3301 ggcagccttg cgcgagggcg cggagatcgc cttctttccg ccggttactg gaggctgaaa
     3361 tggaccagac gcgcgattcg gttcgggttc agggcgccga cttcgacgca gccgccgagc
     3421 ttgccgcgct gcggctcggg cgcaacgaca tcggcgcgat tgcgagcttc gtcgggctag
     3481 tgcgtgacag ccacgccggc gccgaaatcc gcgggatgac gctcgaacac tacccgggga
     3541 tgaccgagaa ggcgcttgaa gcgattgtcg atgaggcccg acagcgctgg tcgctgctcg
     3601 gcgtgcgcgt catccaccgc gtcgggccgc tggcgccggg ggagcagatc gtccttgtcg
     3661 ccgtcgcctc ggcgcaccgc ggcgaggcct tcgacgcctg cgagttcatc atggactacc
     3721 tgaagacgcg ggcgccgttc tggaaacacg agcagactcc cgacggcggc cgttgggtcg
     3781 atgcgcgcga gaccgatgac acggcggcag cgcgctggca gcgcgactga gcggcattcc
     3841 gagaatgcgg aaaaagtctc accgctagaa ggaaaatgat gccaggcggg ggttttcccc
     3901 cgtgttgcgg acatagttcc tcgctatccg atcagcgatg tcaataatgg aaacttcctg
     3961 tatcgccagt cggttttcgt agttcgccga gccagcgagc actgccggca acgctgcgaa
     4021 accgccgatc gccgccatga gatttatcgt tcgcctgggt ttgttctttg ccgttctggg
     4081 tctggccttg ccggcacagg cggtcgatga cgagttgaag gatttgctgg tgtcgatcgg
     4141 ccagtcgcag gaaagcgctg agcgcaccgc gggaggtgtt cgccagcgcg gcgtctgggt
     4201 cgcccgccaa gggccttgct cgcggatcgg tgtgctcatt gggaagccgg aagcgcccga
     4261 gcgaatccag aactaccggg tctgcg
//
LOCUS       DATDAA010000390         2051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_272764,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000390 DATDAA010000000
VERSION     DATDAA010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272764"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(503..1609)
                     /locus_tag="VIS73_11680"
     CDS             complement(503..1609)
                     /locus_tag="VIS73_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY5763857.1"
                     /translation="MRRTLPLLATLLLLTGCGSLVRGSWPAFTSSPLISDAAIRISPG
                     YTLTVEKIVFSAAGAALLYTLYDPLAPNWTIEEETVGEDTYRLTLTMKRFIIGGEGEA
                     LAVLRRWAEAVQHEGRYDAYRIDRFEQGIESTTPVARRYAHATVRMTRQPHPELPAVQ
                     IARADPTVAVDVRERQADGTPSMAQEFTATRAEPLPRPPPRPGGVDDRADRLPAADVE
                     MPKAMAEAPAASSTRLPRPPPIGVSQVMLNARVLFAFDSARLTPAGQQALEREVIANK
                     AELGRLDVLLVSGHTDRIGSYEYNQRLSEARARAVRSFLIGRGFDEKRVLSVGYGKSR
                     PLAGLACDDTLPRGALIECLAPQRRVELDFRAGE"
     gene            complement(1609..>2051)
                     /locus_tag="VIS73_11685"
     CDS             complement(1609..>2051)
                     /locus_tag="VIS73_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HEY5763858.1"
                     /translation="SGDLGVSLVINGTPADGDSFAVQASTPQSLFDTVKNLANVAATP
                     VPGGASGNTLYTGSLGEGLSALDRALDSVLEVRATTGTRLKELDALGESSADLDLQYT
                     GRVSELQDLDYAAALSDFTRRQVQLEAAQRSFVQTARLSLFNFI"
BASE COUNT          336 a          643 c          706 g          366 t
ORIGIN      
        1 ccactctccc tatcggatgc tcaaattgcg aatttttccg tgttggtctg caccacccta
       61 tcggatcaac cacggcaccg acgagacacg atggatacgc atctttcgaa atcaatcgtt
      121 agccggtgct acgcctctat accaagggta ggcgccttgc ctggtaccac attgcgctag
      181 gtcaactgct atgcgcgatt ggtgcatgtc gggcgaaccc atccctatcc cgaccggggc
      241 ttttcaccaa taaaggaacc cgaagccgct atcgaatcca aaaccgcgtg atttgcccca
      301 tatcgaacgc gcttcaggct ccgaaatccc actcgcccac tcagcctgcc tcgatgagga
      361 tcgagttcgg cgcattgcgc gatacaactg tattgaaagg tagatcgcga ttccacagtc
      421 tggtcgcaag ccgacatctt caatgcggtg gctatacgtc cactatgacc gatcacccgc
      481 acttcgctaa tcccgcagcg ctttactccc ccgcccggaa atccaactcg acgcgccgtt
      541 gcggcgcgag gcattcgatc agggcgccgc gcggcagggt gtcgtcgcag gccaagccgg
      601 cgagtgggcg gctcttgccg tagccgacgc tgagcacgcg cttctcgtcg aagccgcggc
      661 cgatcaggaa gctgcgcacg gcacgggcgc gtgcttcgga gaggcgctgg ttgtattcgt
      721 aggagccgat gcggtcggtg tggccgctga ccagcaggac atcgagacgc ccgagttcag
      781 ccttgtttgc gataacttcg cgttcgagcg cctgctgccc ggctggggtc aggcgggcgc
      841 tgtcgaacgc aaacaggact cgggcgttga gcatgacctg gctgacgccg atcggtggcg
      901 ggcgcggcag tcgcgtgctc gacgcggccg gcgcttcggc catggccttc ggcatttcca
      961 cgtcggcggc cggcaggcga tcggcccggt cgtcgacgcc tcccggtcgc ggcggcgggc
     1021 gtggcagcgg ctcggcgcgt gtggcggtga actcctgtgc catcgacggc gtgccatcgg
     1081 cttggcgctc gcggacatcg acggcgacgg tcgggtcggc gcgggcgatc tgcacggccg
     1141 gcagttcggg gtggggctgg cgggtcatgc gcacggtggc gtgggcgtag cgccgggcca
     1201 ccggggtggt cgattcgatg ccctgctcga aacggtcgat gcggtaagcg tcgtagcgcc
     1261 cttcgtgctg cacggcctcg gcccagcggc ggagaacggc gagcgcctcg ccctcgccgc
     1321 cgatgatgaa gcgcttcatg gttaacgtca gccggtaggt gtcttcgccg acggtttctt
     1381 cctcgatggt ccagttcggc gccagcggat cgtagagcgt atagagcagc gcggcgccgg
     1441 cagcgctgaa gacgatcttc tccacggtca gcgtgtagcc cggcgaaatt cggatcgccg
     1501 cgtcggagat cagcgggctc gaggtaaagg caggccagct gccacggacc agcgagccgc
     1561 agccagtcag cagcagcagg gtcgcaagga gtggcagcgt acggcgcatc agatgaagtt
     1621 gaagaggctc aggcgcgcgg tctggacgaa ggagcgctgc gccgcttcga gctgtacctg
     1681 gcggcgcgtg aagtcggata gcgccgccgc gtagtcgagg tcctgcaatt cggagacgcg
     1741 gccagtgtat tgcaggtcga ggtcggccga gctttcgccg agcgcgtcga gttccttgag
     1801 gcgcgtgcca gtggtggcgc gtacttcgag caccgagtcg agcgcgcggt cgagcgccga
     1861 caggccctct ccgaggctgc cggtgtacag cgtgttgccg ctggcgccgc ccggcaccgg
     1921 cgtcgcagcc acgttggcga gattcttgac ggtgtcgaac aggctctgcg gcgtgctggc
     1981 ctgcacggcg aagctgtcgc cgtcggccgg cgtgccgttg atgacgaggc tgacgccaag
     2041 atcgccggaa g
//
LOCUS       DATDAA010000391         2576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_203735,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000391 DATDAA010000000
VERSION     DATDAA010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203735"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /gene="ubiG"
                     /locus_tag="VIS73_11690"
     CDS             complement(<1..671)
                     /gene="ubiG"
                     /locus_tag="VIS73_11690"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEY5763859.1"
                     /translation="MERPLNADPAELEKFSQLAHRWWDPTSEFRPLHEINPLRLEWID
                     GIAGLAGKTMLDVGCGGGILTETAARKGAVVTGIDLSERALKVAQLHLLESGLPVQYA
                     LVSPEEYAQTHAGSFDVVTCMEMLEHVPDPGRTVAACAQLLKPGGQGFFSTINRNPKS
                     YLFAIIGAEYVLKLLPKGTHDYARFIRPSELMGWCRDAGLRPIEVKGMTYNPFTRVYR
                     LGKDC"
     gene            complement(754..1386)
                     /locus_tag="VIS73_11695"
     CDS             complement(754..1386)
                     /locus_tag="VIS73_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY5763860.1"
                     /translation="MNKNMWLSLVAAAGLALSAASFNAIGAAHTMGESKDGHWTDAGK
                     TVWKNGSGQCWKAGYWTPAMAIEECDPDLIKKPGRPAPAPAAKPRPRPVADKVTLAAD
                     VLFDFDKSVIKPEGRARLDDLVSKIGGINLEVVIAIGHTDSIGSDAYNQKLSVRRAES
                     VKAYLVSKGVPANRIYTEGKGEKQPVASNKTRDGRAKNRRVEIEVIGTRK"
     gene            complement(1439..>2576)
                     /locus_tag="VIS73_11700"
     CDS             complement(1439..>2576)
                     /locus_tag="VIS73_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HEY5763861.1"
                     /translation="GLVNAHTHAAMTLMRGLADDLPLMRWLTEHIWPVEMRHVTPEFV
                     RDGTLLACAEMLRGGITCFNDMYFYPEAALQAASTAGMRAALGMIVVDFPTAYASDPD
                     DYLQKGLALRDRYRDDPLASFCLAPHAPYTVSDEALHKVAMLAGELDVPVHIHVHETE
                     DEVARSVAEHGARPLERLRRLGLLGPNFMAVHAVHLSDDDILLLLRHNASVVHCPSSN
                     LKLASGFAPIARIAGDGVNIALGTDGAASNNRLDLLGEMRLAALLGKAVARNAEAIPA
                     HSALRAATLGGAAALGLEPKIGSIVPGKEADLTAVDLSAPELSPCYDPVSHLVYAAGR
                     EHVSHVWVRGRLILEDGAFSGSLTTGLESRLELWQNFIKPGPGR"
BASE COUNT          435 a          839 c          838 g          464 t
ORIGIN      
        1 tcgcagtcct tgccgagccg atacacgcgc gtgaacgggt tgtaggtcat gcctttcact
       61 tcgattgggc gcaggccggc gtcgcggcac cagcccatca actcggaggg gcgaatgaag
      121 cgcgcgtagt cgtgcgtgcc cttgggcagc agcttgagga cgtactcggc gccgatgatc
      181 gcgaacagat aggacttcgg attgcggttg attgtcgaga agaagccctg gccgcctggt
      241 ttcagcagct gggcgcaggc cgcaaccgtg cgtcccggat ccggcacgtg ctcgagcatc
      301 tccatgcagg taacgacgtc gaagctgccg gcgtgcgtct gcgcgtattc ctcgggcgag
      361 accagtgcgt actgtaccgg cagcccgctc tccagcaggt ggagctgcgc gaccttgagg
      421 gcgcgctcgg acagatcgat gcccgtgaca accgcgccct tgcgcgctgc tgtttccgtc
      481 aggatgccgc cgccgcagcc gacgtcgagc atggtcttgc cggcaagtcc ggcgataccg
      541 tcgatccatt ccaggcgcag cggattgatc tcgtgcagcg ggcggaactc gctggtgggg
      601 tcccaccagc ggtgcgcgag ctggctgaat ttctccagct cggccggatc ggcgttcaga
      661 ggacgttcca tgcggaatta ggccaacgcg cggcagtttt gttcggcaaa aaaaaagccc
      721 tgccgtagca gggctttttc gagaggagaa accttacttg cgggtgccga tgacctcgat
      781 ttccacgcga cggttcttcg cgcggccgtc ccgggtcttg ttgctcgcca ccggctgctt
      841 ctcacccttg ccttcggtgt agatgcggtt ggcgggcaca cccttcgaga cgaggtaggc
      901 cttgaccgac tcggcacgac gcaccgacag cttctggttg tacgcgtcgc tgccgatcga
      961 gtccgtgtgg ccgatcgcga tcacgacttc caggttgatg ccgccgatct tcgagaccag
     1021 gtcgtcgagg cgcgcacgcc cttcgggctt gatcaccgac ttgtcgaaat cgaagagcac
     1081 gtcggcggcc agcgtcacct tgtcggcgac cgggcgcgga cgcggcttcg cggcaggcgc
     1141 aggcgccgga cgcccgggct tcttgatcag atcgggatcg cactcctcga tcgccatcgc
     1201 cggcgtccag tagccggcct tccagcactg gccggagccg ttcttccaca ccgtcttgcc
     1261 ggcatccgtc cagtggccgt ccttggattc tcccatcgtg tgcgctgcac cgatggcgtt
     1321 aaacgatgct gccgaaagcg cgagacccgc agccgcgacc agcgacagcc acatgttctt
     1381 gttcatgccg tttcctctct gagctgactt tattgaatgg tccaaaaatt acgttgtttc
     1441 aacgccctgg accaggcttg atgaaattct gccataactc caagcgcgat tccaaccctg
     1501 tggtcaaaga accgctgaac gcgccatcct ccaaaatgag cctgccgcgg acccagacat
     1561 ggctcacgtg ttcccttccg gcagcataga ccaggtgcga caccgggtca tagcatggcg
     1621 agagctcggg ggccgaaaga tccaccgcgg tcaggtcggc ctccttgcca ggcacgatcg
     1681 atccgatctt gggctcgagc ccgagggcgg cggcgccccc tagcgtggcg gcccgcaggg
     1741 cgctatgcgc cggtatcgcc tcggcattcc gggccaccgc cttgcccagc agcgccgcca
     1801 ggcgcatctc gccgagcagg tcgaggcggt tgttgctggc cgcgccatcg gtgcccagcg
     1861 cgatgttgac gccgtcgccg gcgatacgcg cgatcggggc aaagccgctc gccagtttca
     1921 ggttcgaaga gggacagtga actaccgaag cattgtggcg caaaagcaac aaaatatcgt
     1981 cgtccgacag gtgcaccgcg tgcaccgcca tgaaattcgg tcccagcagg ccgagccgac
     2041 gcaggcgttc gagcggtcgc gcgccgtgct cggcgaccga gcgcgccacc tcgtcctcgg
     2101 tttcgtggac atgaatgtgc accggcacgt ccagctcccc ggcgagcatg gcgaccttgt
     2161 gcagcgcctc gtccgacacc gtgtacggcg cgtgcggcgc gaggcagaag gacgcgagtg
     2221 gatcgtcgcg gtagcggtcg cgcagggcga gccctttctg caggtagtca tccgggtcgg
     2281 aggcgtaggc ggtcggaaaa tcgaccacga tcatgccgag cgcggcgcgc atgcccgctg
     2341 tcgacgccgc ctgcagcgcg gcctccggat agaagtacat gtcgttgaag caggtgatgc
     2401 cgccgcgcag catctcggcg caggcgagca gggtgccgtc gcgcacgaat tccggcgtga
     2461 cgtgacgcat ctcgaccggc cagatgtgct cggtcagcca gcgcatcagc ggcaggtcgt
     2521 cggcgaggcc acgcatcagc gtcatcgccg cgtgggtgtg cgcattcacc agcccg
//
LOCUS       DATDAA010000392         8596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_168796,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000392 DATDAA010000000
VERSION     DATDAA010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168796"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1434)
                     /locus_tag="VIS73_11705"
     CDS             complement(115..1434)
                     /locus_tag="VIS73_11705"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HEY5763862.1"
                     /translation="MSCCHHYALAPGGDAVFAVDVSAITYGAGCLGEAGDHAAALGLK
                     RVAVFTDRRLADSPHVHRVLASLAAAGIDAVLYDSVQVEPTDTSFFAAARFAAEGRFD
                     GHVSVGGGSVIDTAKAANLYSTYPAELLAYVNAPIGEARPVPGPLKPHIACPTTCGTG
                     AECTGIAVFDYVAKAVKTGIVSRRLRPTLALIDPETTATLPASVVAASGFDVLSHALE
                     SYTALPHTQRAAAARPSLRPMSQGANPWSDIGCEKALKLTGTYLERAVRDPSDSEARH
                     QMMFAAMLAGIAFGNAGVHLPHGMSYAVAGLVHDFRAPDYPDDVPMVPHGMSVIVNAP
                     AVFRFTADACPERHLDAARWLGADLGGKSDPAAADDAGELVARRLLQLMRATGIPNGI
                     GGVGYGPRDLDALAAGAWAQQRLVRNAPKTVVEADLRRLFNDSLHYW"
     gene            complement(1481..2203)
                     /locus_tag="VIS73_11710"
     CDS             complement(1481..2203)
                     /locus_tag="VIS73_11710"
                     /inference="COORDINATES: protein motif:HMM:NF025221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEY5763863.1"
                     /translation="MAISRRRLLHSLFGGALAGVVTLASSPRALAALPAISAKDAAAG
                     LREALARGARSAVAQLSVRDGFFGDARVRIPLPTQIAQGEKMMRRFGMGRYLDGLSET
                     LNRAAETAVADATPLLLDSLQKMTLADARSIVAGKDDAATQYFRSSAGAALHARFLPV
                     VEVATANLALKPQYEKLSRRAARFGLIGEADADLDNWVTTRTLDGLWLVLGEQERAIR
                     QDPVGTGSALLQRVFGNLGRSR"
     gene            2369..3436
                     /locus_tag="VIS73_11715"
     CDS             2369..3436
                     /locus_tag="VIS73_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEY5763864.1"
                     /translation="MPTPATNAVRLLLLATLVGASTTDAVEPLPALAANAAAVTVSGI
                     SSGGYMAVQYQVAHSQTVSGAAVLAGGPYQCAEGSLWRAMRNCMTPSDWSPAPDLARQ
                     LSALEHAARAGKIDQPEGLRDDRVWLLSGGNDHTVERPVVDALAAFYEHWTAAGALRY
                     LKPPDPGHAMISATDAEANACVTTDPPFINRCPDPDGTGLYDAPKEMLRHLLGPPPEP
                     AAGAGQVVEFDQSAFVRGRPDDAGLAAAGFAYVPAACRQESCRIHVAFHGCRQSAAQI
                     GRRYIDKVGYNAWADRYGLIVLYPQTVPRSGFAFGSWRWLYNPKACWDWWGYTGSDYA
                     TREGSQIKAVKAMIDRLGSVR"
     gene            3537..4463
                     /locus_tag="VIS73_11720"
     CDS             3537..4463
                     /locus_tag="VIS73_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5763865.1"
                     /translation="MSSTHFLYLILLGAIWGGSFLFTRIGIADLGTSWLVMLRVFLGA
                     SFLLLAALALRRNLGLREHWRHWVFLGAMNTAVPFLLFAYGAHELSASLLSIINSTAP
                     MWGVAIGAVLVRGLPGRREIAGLAVGSIGVTLLVLRDPSALQASSALPVFSALLAPLC
                     YGMSSHYARRFTSQLPPFSVALGSQWAAVLCVLPLLAFSGPPAIGSFTAVPVGTWWAA
                     LALGVLCTGIAYLIFFKLIREIGAPSTLTVTFLIPLFGILWGAIFLGEPVSPGTFVGA
                     ACVLLGTAWVTGFSPTRLWQKRRAAPVVVPPA"
     gene            4460..5314
                     /locus_tag="VIS73_11725"
     CDS             4460..5314
                     /locus_tag="VIS73_11725"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY5763866.1"
                     /translation="MNTGHYETVAKAIRYLDAQAELQPALGELAVAVGLSEGHLQRIF
                     VEWAGISPKRFLQALTRERARACLRDSQPLVEAALASGLSGSGRLHDLFVECEGVTPG
                     EVARLGDGLTLGYGFVETPFGRALLASSARGLCHLRFVDSRDAGALAELRNEWPAARL
                     IEDATAAAEIAARVFSGARGSAPGIAPLRLWLKGTNFQFQVWQALLRLPSGTVASYGQ
                     LAHGLAKPDASRAVGNAVGHNPVAWLIPCHRVIRETGALGGYRWGLDRKRTMLARELA
                     MTAEVRVG"
     gene            complement(5327..6367)
                     /locus_tag="VIS73_11730"
     CDS             complement(5327..6367)
                     /locus_tag="VIS73_11730"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HEY5763867.1"
                     /translation="MRPISILLLAATLTATMPLGAETPAALAPGMVNPGYVEPPKWFK
                     ESFLILNEDIAEARQSGRRVMLYFYQDGCPYCAKLVRENFGDKAIAEKTQRHFDVIAL
                     NLWGDREVTDLGGQTMSEKAFAQSIGVQFTPTLLLLDEAGNTALRLNGYTPPHQFSVA
                     LDYVAGQEDKKQKFIDYLRAHATDAASGKLHPQPWLQPSPLELNRRPSGDERPLLVLF
                     EQKVCAGCDEMHREGFPRPEVAGLLKRFRIARVDIAAQEMLTTPAGKRLSMRDWAREA
                     NVVYTPSFAFFDTSGSEVFRVEGYLRPFHLASSLDYVASGGYRTQPEFQRFIEARAAA
                     RRARGERVELMK"
     gene            complement(6492..7211)
                     /locus_tag="VIS73_11735"
     CDS             complement(6492..7211)
                     /locus_tag="VIS73_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY5763868.1"
                     /translation="MKQPRCLKPVWSKGRSARKPAPFWRTLAFLSALGFIGGPAIAVD
                     VGPYVGLGVGGTSYPDATDWVANEVAARVSASSGTPSTASASQKDTGTGAKLFGGYSL
                     SDNASIELTYFDLGETKLTVATTPVVTSSTYTVEGTAFSAALLLSQEVPGDLKLFAKM
                     GLYRADTDITTRRISGTAVVTERESASNTGTHFGLGMTYLMSRTVHLRIEWENLFRVG
                     DDIKTARGDVSMFSASMLHRF"
     gene            complement(7260..>8596)
                     /locus_tag="VIS73_11740"
     CDS             complement(7260..>8596)
                     /locus_tag="VIS73_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEY5763869.1"
                     /translation="SSSGYFRDVQKKVAGFVESGQLGIFAKAYWGHPEYRLPPEANLM
                     AVAHYLDALVWQREVVKLHAIFGGKNPHPNFLVGGAPCAISIDPNNPPKGGGQRGGDA
                     GTALNITGLQTVQHAIRQMREFVDQVYVPDTLAIAGFYKDWFKQGEGVGNFMTLGEFP
                     ARGMADPSGYMIPPGVILDRDLSKIHPIDLDAENEIQEFVSHAWYRYSVGKDKGLHPY
                     RGETALDYTGPKPPYQHLNVEEDYSWLKSPRWRGMPVEVGPLARVLMLYASGHAQTHE
                     LVYKTLAHLDLPIAALYSTLGRTAARTLETKLIADAMQGWYDELVANIRAGDLSTANA
                     SMWEPSSWPARAQGVGVMEAPRGALSHWIVIENQKIANYQAIVPSTWNAGPRDAAGNQ
                     GPYEAALAGHRLHDAKQPIEILRTIHSFDPCIACAVHVAGPDGNELTRIRVV"
BASE COUNT         1329 a         2724 c         2980 g         1563 t
ORIGIN      
        1 gaactcgaag gcgaagttgg gcaggcgtcg gcgcgcgatg ctgcgcaggt cttcgatggt
       61 cagggcgcgg cggaagtcgc ggcagccgtg cgggttgcgg cggtggctca tcgtctacca
      121 gtaatgcagc gaatcgttga acaggcgccg caggtcggcc tcgaccacgg ttttcggcgc
      181 attgcggacg agccgctgct gcgcccaggc tccggcggcg agcgcgtcga gatcgcgcgg
      241 gccgtagcct acgccgccga tgccgttggg aatgccggtg gcgcgcatca gctgcagcag
      301 ccgcctggcg acgagttcgc cggcatcgtc ggcggctgcc ggatcggact tgccgccgag
      361 gtcggcgccg agccagcgtg ccgcgtcgag gtgacgctcg gggcaggcgt cggcggtgaa
      421 gcggaaaacc gccggcgcgt tgacgatgac cgacatgccg tgcggcacca tcggcacgtc
      481 gtcggggtag tcgggtgcgc ggaaatcgtg gaccagaccg gcgaccgcgt agctcatgcc
      541 gtgcggcagg tgcacgccgg cgttgccgaa ggcgattccc gcgagcatgg cggcgaacat
      601 catctggtgc cgggcttcgc tgtcgctggg gtcgcgcacc gcgcgttcga gataggtgcc
      661 ggtgagcttc agtgccttct cgcagccgat gtcgctccac gggttggcgc cctggctcat
      721 tggccgcagg ctaggtcgcg ctgctgccgc gcgttgcgtg tgcggcagcg ccgtgtagct
      781 ctccagcgcg tgtgagagca cgtcgaagcc cgaggcggcg acgaccgagg ccggcagcgt
      841 ggccgtggtt tccgggtcga tcagcgccag tgtcggccgc aggcggcggc tgacgatgcc
      901 ggtcttgacg gccttggcga cgtagtcgaa gaccgcgatg ccggtgcact cggcgccggt
      961 gccgcaagtg gtcgggcagg cgatgtgcgg cttgagtggg ccgggtaccg ggcgtgcttc
     1021 gccgatcggc gcgttaacgt aggcgagcag ctcggcgggg taggtcgagt agaggttggc
     1081 ggctttcgcg gtatcgatca ccgagccgcc gcctaccgag acgtggccgt cgaagcggcc
     1141 ttcggcggcg aagcgcgccg cggcgaaaaa ggacgtatcg gtcggttcga cctgcaccga
     1201 gtcgtagagc acggcgtcga tgccggcagc ggcgagcgag gcgagcacgc gatgcacatg
     1261 cggcgaatcg gcgaggcgtc tgtcggtgaa cacggcgacc cgcttgagcc cgagcgccgc
     1321 ggcatggtcg ccggcttcgc cgagacagcc ggcgccgtag gtgatcgccg agacgtcgac
     1381 ggcgaagacg gcatcgccgc cgggtgccaa tgcgtagtgg tgacagcaac tcatagcctt
     1441 acctctactt gtgtctgccg gggtgcaggc gacacgccgc tcagcgcgag cgacccagat
     1501 tgccgaatac ccgctgcagc agggcgctgc cggtgccaac cgggtcctgg cgaatcgcgc
     1561 gctcctgctc gccgagaacc agccatagcc cgtcgagcgt gcgcgtcgtg acccagttgt
     1621 cgagatcggc atcggcctcg ccgatcaggc cgaaacgcgc ggcacggcgc gagagttttt
     1681 cgtattgcgg tttcagcgcc aggttcgcgg tcgcgacttc gacgaccgga agaaaacgcg
     1741 cgtgcagcgc cgcgccggcg ctactgcgga agtattgcgt cgcggcatcg tccttgccgg
     1801 cgacgatcga gcgagcgtcg gccagcgtca tcttctgcag cgagtcgagt agcagcggcg
     1861 ttgcatcggc gaccgcggtt tcggcggcgc ggttaagcgt ttccgaaaga ccgtcgaggt
     1921 aacggcccat gccgaagcgg cgcatcatct tctcgccctg agcaatctgt gtcggcagcg
     1981 gaatgcgcac gcgggcgtcg ccgaagaaac cgtcgcgaac tgagagctgg gcgaccgcgg
     2041 atcgggcacc acgcgccagc gcttcgcgga gcccggccgc tgcgtccttg gcgctgatcg
     2101 cgggcagcgc ggccagcgcc cggggcgagc tggcgagcgt gacgacaccc gccagcgcac
     2161 cgccaaacag cgaatgcagc aatcgtcgcc ggctaattgc catgtcacgg aactcccttc
     2221 tgctcggtcg gggcattcta aaggctatga ccttggtccg gggccgtcgc gtaaattgca
     2281 cttgtcattg ttgcgctatg actcaagaat gactgtgacg atgccgttga acgtattgcc
     2341 tcaagggata tcccactaca agttgccgat gcccacgcct gccaccaacg ctgttcgtct
     2401 cctcctgctc gccaccctgg tcggcgcatc gacgacagat gccgtcgagc ccttgccggc
     2461 actcgccgcc aacgcggcgg cggtcaccgt gtcggggatt tcttccggtg gctacatggc
     2521 cgtgcagtac caggttgcgc actcgcagac cgtcagcggc gccgccgtgc tcgccggcgg
     2581 gccctatcag tgtgccgagg gttcgctctg gcgcgcgatg cgcaactgca tgacgccgtc
     2641 ggactggtcg cctgcccccg atctcgcacg ccagttgtcg gcactagagc atgcggcccg
     2701 cgcgggaaag atcgatcagc ctgagggcct gcgcgacgac cgcgtctggc tgctgtcggg
     2761 cggaaacgac catacggtcg agcgaccggt agtcgatgca ctggcggcgt tttacgagca
     2821 ctggacggca gccggcgcgc tgcgctacct gaagccgccc gaccccgggc acgcgatgat
     2881 ttcggcgacc gatgccgagg cgaatgcctg cgttacgacc gatccaccat tcatcaaccg
     2941 ctgtcccgac ccagacggca ccggcttgta cgatgccccg aaggaaatgc tccgacatct
     3001 gctcggtccg ccgccggaac cggctgccgg cgcggggcag gtggtcgagt tcgaccagag
     3061 cgctttcgtt cggggtcgtc ctgacgacgc tggcctcgcc gcggccgggt tcgcctacgt
     3121 gccggccgcc tgccgccagg agtcgtgtcg gatccacgtc gcattccacg gctgccggca
     3181 aagcgccgcg cagatcggtc gtcgctacat cgacaaggtc ggctacaacg cctgggcgga
     3241 tcgctacgga ctgatcgtgc tttatccgca gaccgtgccg cgcagcggct tcgctttcgg
     3301 ctcctggcgc tggctgtaca accccaaagc ctgctgggac tggtggggct ataccgggag
     3361 cgactacgcc acccgcgagg gctcgcagat caaggccgtc aaggcgatga tcgaccgcct
     3421 cggcagcgtg cgctgaaagc gggtcgacag gggcctccgg ccgcccacgg taattaagtt
     3481 cgccgatcgc aggattcggg tcgccaagcg tgcgtccggc tgccagaatc ctcgccatgt
     3541 cttccacgca cttcctctat ctgatactgc tcggcgccat ctggggcggc tctttcctgt
     3601 tcacccggat cggcatcgcc gacctcggca ccagctggtt ggtgatgctg cgggtcttcc
     3661 taggggccag cttcctgctg ctggcggcgt tggccctgcg ccgcaacctc ggattgcgcg
     3721 agcactggcg gcactgggtt tttctcgggg cgatgaacac cgccgttcca tttctgctgt
     3781 ttgcctacgg cgcgcatgag ttgagtgcct cgttgctgtc gatcatcaac tcgacggcgc
     3841 caatgtgggg cgtcgccatt ggcgccgtgc tggtccgtgg cctgccggga cgccgcgaga
     3901 tcgccgggct cgcggtcggc agcatcggcg tcacgctgct ggtcctgcgc gacccgagtg
     3961 cgctgcaggc gtcgtcggcg ctgccggttt tttcggcatt gctggctccg ctttgctatg
     4021 gcatgtcgag tcattacgcg cggcgcttca ccagccagtt gccgccgttt tcggtagcgc
     4081 tcggcagcca gtgggcggcg gtcctttgcg tcctgccgct gctcgccttc agcgggccgc
     4141 cggcgatcgg cagcttcacg gcagtgcccg tcggcacctg gtgggcagcg ctggcgctcg
     4201 gagtgttgtg caccggtatc gcctacctga tcttcttcaa gctgatccgc gagatcggcg
     4261 cgccgtcgac gcttacggtg accttcctga tcccgctatt cggcattctc tggggcgcga
     4321 tcttcctcgg cgagccggta tcgcccggca ccttcgtcgg cgctgcctgc gtgctgctgg
     4381 ggacggcctg ggtcaccggc ttcagcccaa cgcgcctgtg gcagaagcga cgcgccgcgc
     4441 cggtggtggt accgccggca tgaacaccgg ccattacgaa acggtcgcga aggcgatccg
     4501 ctacctcgac gctcaggcgg aattacagcc cgccctcggc gagctcgcgg tggcggtcgg
     4561 actctcggaa gggcacctgc agcgcatctt tgttgaatgg gcagggatca gccctaagcg
     4621 attcctgcag gcgctgacgc gcgaacgagc gcgcgcctgc ttgcgcgatt cgcagccgct
     4681 ggtcgaggca gcgctggcca gcgggctgtc gggcagcggg cggctgcacg atctgttcgt
     4741 cgagtgcgag ggggtgaccc ccggcgaggt cgcgcggctc ggcgacggac tgacgctggg
     4801 ctacggattc gtcgagacac cgtttggccg ggccttgctg gcgagcagtg cgcgcggtct
     4861 ttgtcatctg cgcttcgtcg acagtcgcga tgccggcgca ctggccgaac tgcgcaacga
     4921 atggccggcg gcacggctga tcgaggacgc gacggccgct gcggaaatcg ccgcgcgggt
     4981 cttctccggt gcccgtggca gtgcgccggg gattgcgccg ctacggcttt ggctgaaggg
     5041 gaccaacttt cagttccagg tctggcaggc gctgctgcgc ctgccttccg ggacggtggc
     5101 gagctacggc caactggcgc atggcctagc gaaacccgat gccagccgcg ccgtcggcaa
     5161 cgcggttggc cacaacccgg ttgcctggct gattccctgc caccgcgtca tccgcgaaac
     5221 cggcgcactc ggcggctacc gctggggact cgatcgcaag cggaccatgc tggctaggga
     5281 actggcgatg accgccgagg tccgcgtcgg atagcgactt ttcggtctac ttcatcagtt
     5341 cgacgcgttc gccgcgggcg cggcgtgccg ctgcgcgggc ctcgatgaag cgctgaaact
     5401 ccggttgcgt tcggtaaccg cccgatgcca cgtagtcgag cgaagaggca aggtggaagg
     5461 ggcgcaggta gccttcgacg cggaagacct cgcttccgct tgtatcgaag aaggcgaagc
     5521 tcggtgtgta gaccacgttc gcttcgcgcg cccagtcgcg catcgacagc cgcttgccgg
     5581 caggcgtcgt cagcatttcc tgcgctgcga tgtcgacgcg ggcaatgcgg aaacgtttga
     5641 gcaagccggc gacctcgggc cggggaaatc cctcgcggtg catctcgtcg cagccggcgc
     5701 agaccttctg ttcgaagagc accagcaggg gccgctcgtc gcccgagggc cggcggttca
     5761 gctccagcgg cgaaggctgc agccagggct ggggatggag cttgccgctt gccgcatcgg
     5821 tggcatgggc gcgcaggtag tcgatgaatt tctgtttctt atcctcttgc ccggcgacgt
     5881 agtcgagcgc aaccgagaat tggtgcggcg gcgtgtagcc attgagtcgc aaggcggtgt
     5941 ttccggcctc gtcgagcagc agcaaggttg gcgtgaactg gacgccgata gactgcgcaa
     6001 aggccttctc gctcatcgtt tgaccgccga gatcggtgac ctcgcggtct ccccagagat
     6061 taagcgcgat gacgtcgaag tgccgttgcg tcttctcggc gattgccttg tcgccgaagt
     6121 tctcgcgaac cagcttcgcg cagtaggggc agccgtcctg atagaagtac agcatcacgc
     6181 gccgtcccga ctgccgggcc tcggcgatgt cttcattaag gatcaggaag gattccttga
     6241 accatttcgg cggttcgacg tacccgggat tcaccatgcc cggcgccagc gcagcggggg
     6301 tttcggcgcc aagcggcatc gtcgccgtca gggtagcggc cagcaacagg atcgaaatcg
     6361 gtcgcatggg agttttccag ggagtcgcga aggccgttgc cagtcggctg gaagagcagt
     6421 ttcagcgtat caattccgcg ccgtttcgcc accccccgaa cggggcatgg cggtcggctg
     6481 cgagaatggt ttcagaatcg gtggagcatc gaggccgaga acatcgatac atcgccgcgc
     6541 gcggtcttga tgtcgtcgcc cacgcggaag aggttctccc actcgatgcg caagtggacg
     6601 gtccgggaca tcaagtaggt catgccgagt ccgaagtgag tgccggtatt ggaggccgac
     6661 tcgcgctcgg tgacgactgc ggttccgctg atgcgacgtg tggtgatgtc ggtatcggcg
     6721 cgatagagcc ccatcttggc gaacagtttg agatcgccgg gaacctcctg cgagagcagc
     6781 agggctgcag aaaaggcggt tccttcaacg gtgtaggtcg acgacgtgac aaccggcgtg
     6841 gtggccacgg tgagcttggt ttcgccaagg tcaaaatacg tgagttcgat cgaggcgttg
     6901 tcgctcagcg agtagccccc gaaaagcttg gcgccggtac ctgtgtcctt ctggctggcc
     6961 gaagcggtgc tcggcgttcc cgacgaggcg ctgacccggg cagcgacctc gttcgcgacc
     7021 cagtcggtgg catcgggata gctggtcccg ccgacgccga gtcctacata agggccgacg
     7081 tcgacggcaa tggccggccc gccgatgaat ccgagcgccg aaaggaatgc gagcgtccgc
     7141 cagaagggcg ccggctttct ggcggaccgg cctttgctcc aaaccggttt gaggcaacgt
     7201 ggctgtttca tgatcatctc ctttgagaag tggcagattg ccaatttgcg gcgagctgcc
     7261 tagacgactc ggatgcgagt caattcgttt ccgtcggggc cggcgacatg cacggcacaa
     7321 gcgatgcagg gatcaaagct gtgaatcgtg cggaggattt cgatcggctg cttcgcgtcg
     7381 tggagacggt ggccggccaa cgcggcttcg tagggaccct gattacctgc cgcgtcacgc
     7441 gggccggcgt tccaggtgct cggcacgatc gcctgatagt tggcgatctt ctggttttcg
     7501 atgacgatcc agtgcgacag cgcgccgcgt ggagcctcca tgacgccgac gccctgggcg
     7561 cgtgccggcc agctgcttgg ttcccacatg ctggcgttgg ccgtcgatag gtcgccggca
     7621 cggatgttgg caaccagttc gtcgtaccag ccctgcatcg cgtccgcgat cagtttcgtt
     7681 tccagcgtcc gggcggcggt gcgaccgagc gtcgagtaca gcgctgcaat cggtaagtcg
     7741 aggtgtgcca gcgtcttgta gacaagttcg tgcgtctgcg cgtgcccgga cgcgtagagc
     7801 atcagcaccc gtgccagcgg cccgacctcg accggcatgc cgcgccagcg tggcgacttg
     7861 agccaggagt aatcctcttc gacattgagg tgctggtagg gcggtttggg tcccgtgtag
     7921 tcaagcgcgg tctcgccgcg ataaggatgc aggcctttgt ccttgccgac cgagtagcgg
     7981 taccaggcgt ggctgacgaa ctcctggatt tcgttttcgg cgtcgaggtc gatcgggtgg
     8041 atcttcgata ggtccctgtc gaggatcacg cccggcggaa tcatgtagcc gctcggatcg
     8101 gccatgcccc tggccgggaa ttcgcccagc gtcatgaagt tgccgacgcc ttcgccctgc
     8161 ttgaaccagt ccttgtagaa gccggcgatt gccagcgtgt ccggcacgta gacctgatcg
     8221 acgaattcgc gcatctgccg gatggcgtgc tgcacggtct gcaggccggt gatgttgagc
     8281 gccgtccccg catctccgcc gcgctggccg ccacccttgg gcgggttgtt cgggtcgatg
     8341 ctgatcgcgc agggcgcgcc gccgacgagg aagttagggt gcggattctt gccgccgaag
     8401 attgcgtgca gcttgaccac ctcgcgctgc cagaccagtg cgtcgagata gtgggcgacg
     8461 gccatcaggt tcgcctcagg cggcagccgg tattcggggt gtccccagta ggctttcgcg
     8521 aaaatgccca gctgccccga ctcgacgaag ccggcgacct ttttctgtac gtcccggaag
     8581 tagcccgagg atgagc
//
LOCUS       DATDAA010000393         2549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_413775,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000393 DATDAA010000000
VERSION     DATDAA010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413775"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VIS73_11745"
     CDS             complement(<1..283)
                     /locus_tag="VIS73_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5763870.1"
                     /translation="MDFDVVVVGGGPAGLAAAIRLKQRARESGQEISVCLIDKGSEIG
                     AHILSGAVIDPQALNELLPDWQSLGAPLDTQVSEDRFLILGESSAFRVPE"
     gene            436..846
                     /locus_tag="VIS73_11750"
     CDS             436..846
                     /locus_tag="VIS73_11750"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY5763871.1"
                     /translation="MSADRQLVHRTEIPIRWGDLDAYGHVNNTVFFRFMEQARVEWLA
                     TLSHALKPHGDGPVIINAACTFLVQLNYPGTVGVELYVGPPGRSSIPTYYEIRCEGVL
                     VAEGESKVVWMDFASGKSVALPAAIRSRLPGADT"
     gene            887..1681
                     /locus_tag="VIS73_11755"
     CDS             887..1681
                     /locus_tag="VIS73_11755"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY5763872.1"
                     /translation="MNSQAEDPYIERTDRADGVTTLTLNRPAQFNPLSTAMLSAVQAA
                     LDTIAADPAIRVVVIAGQGKAFCAGHDLREMRANPDKAFQQALFRQAGKLMMTLTRLP
                     QPVIARVHGVAAAAGCQLVAMCDLAVAADVAKFSVPGINVGLFCSTPAVGLSRNMGRK
                     QAMEMLLTGEFIDALEAQRRGLVNRVAPADALDAEIERLVASILAKSPAAISMGKRLF
                     YDQGEMGLDGAYQLAAETMACNMMLEDAAEGIDAFSEKRKPTWKGK"
     gene            1886..2188
                     /locus_tag="VIS73_11760"
     CDS             1886..2188
                     /locus_tag="VIS73_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5763873.1"
                     /translation="MSAFSQFSIAGDFDEKAAKKTIKNNDCGKCHAEKKTKKGPSYKK
                     TAADYAGKADAYAKISKHLREEPMVKLEDGTEEKHKAIEVENEAELMNLVNYILAQ"
     gene            2271..>2549
                     /locus_tag="VIS73_11765"
     CDS             2271..>2549
                     /locus_tag="VIS73_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEY5763874.1"
                     /translation="MKRVGAALILSALVGGMSLPVFAMDEAKDIVLVGDAKCTKCHDE
                     ADDPKVLAVGKTRHGVNADKRTPTCTSCHGDSEDHIAKAGRGGEPAPKV"
BASE COUNT          544 a          777 c          785 g          443 t
ORIGIN      
        1 attcggggac ccggaaggct gaggattcgc cgaggatcag gaagcggtcc tcgctcacct
       61 gcgtatcgag tggggcgcca agcgattgcc agtcggggag caactcgttt agcgcctgcg
      121 gatcgatgac ggcgccggac aggatatggg cgccgatctc cgagcccttg tcgatcaggc
      181 agacggaaat ctcctgtccg ctctcgcggg cgcgctgctt gagccgaatt gccgccgcca
      241 ggccggctgg gccgccgccg acgaccacca catcaaaatc catcgcctcg cgttcggatt
      301 cgactgcttc gctcaccgca ttctccccgc ttcggattgg ttatcaccgc atggtcttgc
      361 catgctgccg cgagtctata acatacgcta cagtatggga catcgcccga agccgggcag
      421 cacagggatt cccgcttgag cgctgatcga caactcgtcc accgcactga aatccccatt
      481 cgctggggcg atctcgatgc ctacggacac gtcaacaaca cggtcttctt ccgcttcatg
      541 gagcaggcgc gggtcgaatg gctggccacg ctgagccacg cgttgaagcc tcacggagac
      601 gggccggtca tcattaacgc cgcctgcacg tttctcgtac agttgaatta cccgggaacc
      661 gtcggcgtcg agctttacgt cgggcctccc ggccgcagca gcattcccac ctactacgag
      721 atccgctgcg agggcgtctt ggtggccgag ggcgagtcga aagtggtgtg gatggatttc
      781 gcaagcggca agtcagtggc gctgcccgct gcaatccgat cccgcctgcc cggtgccgat
      841 acttgagccc gcggccccac ccgttcccgg taccagagga gagatcatga acagccaggc
      901 cgaagatccc tacatcgaac gcaccgaccg agcagatggc gtgaccacgc tgacgctcaa
      961 ccggccggcc cagttcaacc cgctgtcgac ggccatgctc tcggcggtgc aggcggcgct
     1021 cgacacgatc gctgccgatc cggcgatccg cgtcgtcgtg attgccggcc agggcaaggc
     1081 cttttgcgcc ggacacgacc tccgcgaaat gcgcgccaac ccagacaagg ccttccagca
     1141 ggcgctgttt cgccaagccg gcaagctgat gatgacgctc acgcgccttc cgcagccggt
     1201 catcgcgaga gtgcacggcg ttgccgccgc ggccggttgc cagctggtgg cgatgtgcga
     1261 tctggcagtc gccgccgatg tcgcgaagtt ctccgtgcct ggaatcaacg tgggcctgtt
     1321 ctgttcgacg ccggcggtcg gcctgtcgcg caacatggga cgcaaacagg cgatggaaat
     1381 gctgcttacc ggggaattca tcgacgccct tgaggcgcag cgccgcggct tggtcaaccg
     1441 tgtcgcaccc gccgacgcgc tcgacgccga gatcgagcgg ttggtcgcga gcattctggc
     1501 gaagagtccc gctgccattt ccatgggcaa gaggctcttc tacgatcagg gcgaaatggg
     1561 cctcgatggc gcctaccagc tcgccgccga aaccatggcc tgcaacatga tgcttgaaga
     1621 cgcggctgag ggaatcgacg ctttctcgga gaagagaaag ccgacgtgga agggaaagtg
     1681 attgacttcc ctcaagtgcg catcttcttc ctgcagagta caatttcgcg cgacgtcaga
     1741 aaaagatgct gagcaatagt ctcagtgggt ttcggtaaag ggatcaatat ggggcgcaag
     1801 gtacgccgag aactttcggt gtcagcatgg agacgatgtg aacaattttc gtagaatcac
     1861 acttcaatcg gcgctgatcg ttctggtgag tgctttctcc cagttcagca ttgccggcga
     1921 cttcgacgag aaggcagcca agaaaacgat taagaacaac gactgcggta agtgccacgc
     1981 cgagaaaaag accaagaaag gtccgtcgta caagaaaacc gctgccgact acgccgggaa
     2041 ggcggacgct tacgccaaga tcagcaagca cctgcgcgag gagccgatgg tcaagctcga
     2101 agacggcacc gaagaaaaac ataaggccat cgaagtcgag aacgaggccg agcttatgaa
     2161 tctggtcaat tacatcctgg cccaatagcc tgcccggaca ccggacgaat tcgaaactgc
     2221 aatagacaat ccgcgtgcag catcggcgtg gaaacggagg gagtgaaaag atgaaacgag
     2281 tcggtgcagc attgattctt agcgcgctgg tcggcggcat gtcgctgccg gtcttcgcga
     2341 tggacgaggc caaggacatc gttctggtcg gcgacgccaa gtgtacgaag tgccacgacg
     2401 aagccgacga tccgaaggtg ctcgcggtcg gcaagacccg tcacggcgtc aacgctgaca
     2461 agcggacacc aacctgtacc agctgtcacg gagacagcga ggatcacatc gccaaggccg
     2521 gccgcggtgg cgaaccggcg ccgaaggtc
//
LOCUS       DATDAA010000394         5022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_238677,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000394 DATDAA010000000
VERSION     DATDAA010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238677"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VIS73_11770"
     CDS             complement(<1..486)
                     /locus_tag="VIS73_11770"
                     /inference="COORDINATES: protein motif:HMM:NF019977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HEY5763875.1"
                     /translation="MSVILEVEHVTTYRYSKPVEFGLHRVAFHPRAAHDIRVLYADLS
                     VSPHSRQHWIHDVFSNSVAVVEPLVPAETLSFTARFAIEHLGVKNQELPVDQDAKLYP
                     FQYSDDDRLNLSSFLPLQYPNDAAAVSGWVAGFLPQRGTIHTRDVLSNINQFVRTDFT
                     YA"
     gene            complement(591..1376)
                     /gene="rsmA"
                     /locus_tag="VIS73_11775"
     CDS             complement(591..1376)
                     /gene="rsmA"
                     /locus_tag="VIS73_11775"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY5763876.1"
                     /translation="MNRHIPRKRFGQHFLVDRAIVDAIVEAIAPREGETLIEIGPGLG
                     ALTEPLLAKIDRLHVVEIDRDLIAHWHCHREAARVIVHASDALAFDFGALGNALRIVG
                     NLPYNISTPLLFHLADFATQVRDMHFMLQKEVIERMVAAPGDSARGRLSVMLQARYRM
                     DWLLDVPPESFDPPPKVDSAVVRMIPLAASERVDRAAPAAFSRVVAAAFGQRRKMLRN
                     TLRGLIDAEALQGLGVDPQARAEELSVDEFAAIAAALGPSDAV"
     gene            complement(1373..2395)
                     /gene="pdxA"
                     /locus_tag="VIS73_11780"
     CDS             complement(1373..2395)
                     /gene="pdxA"
                     /locus_tag="VIS73_11780"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY5763877.1"
                     /translation="MPPTSKSASKSRDRNLVPASSRPLIAVTSGEPAGIGPELCARLA
                     GRFPQARLVVLGDRELLAARGGAEVWRDYAAAADRGDSRLECLHLPLAVPSVASRLDV
                     ANADYVIALLDRAIAGCVAGEFAAMVTAPVHKGVIADSGRAFSGHTEYLAERTATQRV
                     VMMLTGGGLRVALVTTHLALSAVPAAVTATAIDETVRIVHRELQAKFGIAAPRILVAG
                     LNPHAGEGGHMGREEIEVIAPALERLRGEGINAVGPLPADTLFTRDVLAGSHAQLAMY
                     HDQGLAVLKYASFGHGTNVTLGLPIIRSSVDHGTALDLAGTGRADPGSLFAAVEQAID
                     MVSRRV"
     gene            complement(2358..3686)
                     /locus_tag="VIS73_11785"
     CDS             complement(2358..3686)
                     /locus_tag="VIS73_11785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5763878.1"
                     /translation="MSARITASRLIVTLSLLLVTTAFAASKDATPAKPPPTVEIESIA
                     AVVNTEVVTQRELDARLAAARRQLSRQNIAIPPNEALLRQVLERLILERVQLQVARDV
                     GIRVDEAQLDQSIARIAESNKMTLAAFRKAVEADGIPWVRFREDLRHEILLARVRDRE
                     VDNRVSVSDAEVDHAFEIEQRLGKDRQEVDLGHIIVRVPESGDPTQVATLRTRIDEAR
                     RKLLAGEDFARVAATYSDAADALQGGRIGVRGLDRLPSLYAEVARSLAVDGVSEVLRS
                     AAGFHVLKLFARRGATEEPVRQTHVRHILIRPDEILSDGDVQQRLRGFRERIVNGDDF
                     GDIARSNSQDGSAPKGGDLGWVYPGVTVPDFEQAMDALAINEVSKPVRSPFGWHLIQV
                     LERRMAPVPPDRQKDLLRQALRERKADEAYQEWLRQIRDAAYVEIRIEIS"
     gene            complement(3697..>5022)
                     /locus_tag="VIS73_11790"
     CDS             complement(3697..>5022)
                     /locus_tag="VIS73_11790"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEY5763879.1"
                     /translation="YYRDLSSRASTSAQTQLVRQGLLGWSAGAWTADIKATRYQTLQP
                     DPANLVARPYELMPQFNLTGWQALAGDLTGVLTAQYTDFQHPDSDRIEARRTVLYPQL
                     AWPNVRPWGYVTPKIGLHLTRYSFAGDAAEAAGLPDAYRREVPIASLDSGLVFERQSR
                     WFDKDLTQTLEPRLFYLYVPLRDQSFLTNNQINFDSGIADFNFAQIFSENLFTGQDRI
                     ADANQLTVAVGSRFIEPDNGVERLNVTLGQRFYFAPQTVTLSPGDSRRTERKTDLLGA
                     ASGRVSDSVWVDGGMQYNPRDGRMERSYLGTRWLAGTGRSLSASYRLVREQTAPFDIA
                     IEQLDIAGQWPIFGGWQGVGRYNYAIDESRPIEVIAGLEYRADCWSVRTVLQRYATTA
                     DDTVSAVFIQLELTDFSRIGSSPLELLRRSVPGYGRADQSVSDPVFGNP"
BASE COUNT          886 a         1572 c         1742 g          822 t
ORIGIN      
        1 tgcataggtg aagtccgtgc ggacgaactg gttgatgttg gacagcacgt cacgggtatg
       61 gatggtgcca cgctgcggca ggaagcccgc gacccatcca ctgacggcag cggcatcgtt
      121 cggatactgc agcggcagaa aactcgacag gttcaaccga tcgtcgtctg agtactggaa
      181 gggatagagc ttggcgtcct gatcgaccgg gagctcctga ttcttcacgc ccaggtgctc
      241 gatggcaaag cgcgctgtaa agctcagcgt ttcggctggc accagcggct cgaccaccgc
      301 caccgagttg gagaacacgt cgtgaatcca gtgctggcgg ctatgcggcg aaacgcttag
      361 gtcagcgtac agcacccgga tgtcgtgggc ggcgcgcggg tgaaaagcca cgcggtggag
      421 accgaattcc accggcttgg aataccggta agtggtgacg tgttcgactt caagaatcac
      481 ggacatgaat gcgggccgga aattgggggg cgcccatcat cgccgattag ggctgaaggc
      541 aaaggccggc gcgcggattg ccggcccggc cgttgatcac accctcgaaa tcagacagcg
      601 tcgctggggc ccagcgccgc cgcaatcgcg gcaaattcgt cgacgctcag ctcctcggcg
      661 cgcgcctggg ggtcgacgcc gagcccctgc agcgcctcgg catcaatcag cccgcgcagc
      721 gtgttgcgca acatcttgcg gcgctggccg aaggcggcgg caacgacccg ggagaatgcg
      781 gcgggcgcag cacgatcaac acgctcgctc gcagccaacg gaatcatgcg cacgacggcc
      841 gaatcgacct tgggtggcgg gtcgaaggac tcgggcggca cgtcgagcag ccagtccatc
      901 cggtagcgcg cctgcagcat caccgacagc cggccacgcg ccgagtctcc gggagcggcg
      961 accatgcgct cgatgacttc cttctgcagc atgaagtgca tgtcacgcac ttgggtggcg
     1021 aagtcggcga ggtggaagag cagcggcgtc gagatgttgt aggggagatt gccgacgatt
     1081 cgcagcgcgt ttccgagcgc gccgaagtcg aaggcgagcg catcgcttgc gtgcacgatg
     1141 acgcgcgccg cctcgcgatg gcagtgccaa tgagcgatca ggtcgcggtc gatctcgacc
     1201 acgtgcagac ggtcgatttt cgccagcagc ggttcggtca gcgcgccgag gccagggccg
     1261 atctcgatca gcgtctcgcc ctcgcgcggc gcgatcgcct cgacgatggc atcgacaatc
     1321 gcgcgatcga ccaggaaatg ctggccaaag cgcttgcgtg gaatgtgccg gttcatacgc
     1381 ggcgcgacac catgtcgatc gcctgctcga cggcggcaaa gaggctgcct ggatcggcac
     1441 ggccggtgcc ggctaggtcg agcgcggtac cgtgatcgac cgagctgcgg atgatcggca
     1501 ggccgagcgt gacgttggtg ccgtggccga agctcgcgta cttgagtacg gctagcccct
     1561 gatcgtgata catggcgagc tgcgcgtgcg agccagccag cacgtcgcgc gtaaacagcg
     1621 tgtcggccgg taaggggccg acggcattga tgccttcgcc gcgcaggcgt tcgagcgccg
     1681 gcgcaatcac ctcgatctcc tcgcggccca tgtggccgcc ctcgccggcg tgcgggttga
     1741 gtccggcgac gaggattcga ggagcggcga tgccgaattt cgcctgcagt tcgcggtgga
     1801 cgatgcgcac ggtctcgtcg atggccgtcg cggtaaccgc cgcgggaacc gcggatagcg
     1861 ccagatgcgt ggtgaccagc gcaacgcgca ggccgccgcc ggtgagcatc atcaccacgc
     1921 gctgcgtcgc agtgcgctcg gcgaggtatt cggtatggcc gctgaaggcc cggcccgagt
     1981 cggcgatcac gcctttgtgc acgggtgcgg tgaccatcgc ggcaaactcc ccggcgacgc
     2041 agccggcgat ggcgcgatcg agcagggcga tgacgtagtc ggcattggcg acatcgaggc
     2101 gactggcgac agacggcacg gcgagcggca gatgcaggca ctctaggcgg gagtcgccgc
     2161 ggtcagcggc ggctgcgtag tcgcgccaga cctcggcacc gccgcgtgca gcgagcagct
     2221 cgcgatcacc gagtaccacc agccgcgcct gcggaaaacg tccggccagt cgcgcgcaga
     2281 gttcggggcc gatgccggcc ggctcgccgc tggtaaccgc gatcagcgga cgcgaacttg
     2341 cgggcacgag attccggtca cgagatttcg atgcggattt cgacgtaggc ggcatcgcga
     2401 atctgccgca gccattcctg ataagcctcg tcggccttgc gttcgcgcag tgcctgccgc
     2461 agcaggtcct tctggcggtc gggcggcacc ggcgccatgc gccgttcgag cacctggatc
     2521 aggtgccagc cgaagggcga gcgtacgggc ttgctgacct cgttaatcgc cagcgcatcc
     2581 atcgcctgct cgaaatcggg aacggtgaca ccgggataga cccagccgag gtcgccaccc
     2641 ttgggcgccg atccgtcctg cgaattgctg cgcgcgatat cgccgaaatc atcgccatta
     2701 acgatgcgtt cgcgaaagcc gcgcaggcgc tgctggacgt cgccgtcgga aagaatctcg
     2761 tcggggcgaa tcaggatgtg ccgcacatgc gtctgccgaa ccggctcttc ggtcgcgcca
     2821 cggcgcgcga acaacttgag cacatgaaag cccgcggcgc tgcgcagcac ctctgacacg
     2881 ccgtcaacgg ctaggctgcg cgccacctcg gcgtagagcg agggtaggcg gtcgagacca
     2941 cggacgccga tgcggccgcc ctgcaaggcg tcggcggcat ccgaataggt cgccgcaacg
     3001 cgggcgaaat cctcgccggc aagcagcttg cgtcgcgctt cgtcgatgcg cgtgcgcaac
     3061 gttgccacct gcgtcgggtc gccgctctcg ggaacgcgga cgatgatgtg gccgaggtcg
     3121 acctcctgcc ggtccttgcc cagccgctgc tcgatctcga aggcatgatc gacctcggca
     3181 tctgaaacgc tgacgcggtt gtcgacctcg cggtcgcgta cgcgggcaag taggatttcg
     3241 tgccgcaggt cctcgcggaa gcgcacccag ggaatcccgt cggcttcgac ggccttgcga
     3301 aacgctgcca gcgtcatctt gttcgactcg gcgatgcggg ctatcgattg atcgagctgt
     3361 gcctcgtcga cgcggatgcc cacgtcccgg gcgacctgaa gctgcacgcg ctcgaggatc
     3421 agccgctcga gcacctggcg cagcagggcc tcgttgggcg ggatcgcgat gttctgccgc
     3481 gacagctggc ggcgcgccgc ggccagtctg gcgtcgagtt cgcgctgggt gacgacttcg
     3541 gtattgacga cggcagcgat gctctcgatc tcgacggtcg ggggcggctt cgccggcgtg
     3601 gcgtccttac tggcggcgaa ggccgtcgtc accagcagca gcgacagggt gacgatgagt
     3661 cgggaagcag tgattctggc tgacatcgat gatctctcag gggtttccaa aaaccggatc
     3721 ggacaccgac tggtcggccc gtccgtagcc cggcacgctg cgccgcagga gttccagcgg
     3781 actcgagccg attcgcgaga agtcggtgag ttcgagttgg atgaagacgg ccgacacggt
     3841 atcgtcggcc gtcgttgcat agcgctgcag gacggtcctt accgaccagc aatcagcgcg
     3901 gtattcgaga ccggcgatga cttcgatcgg ccgactctcg tcgatcgcgt aattgtagcg
     3961 cccgacgccc tgccagccac cgaagatcgg ccactgcccg gcgatgtcga gctgctcgat
     4021 ggcgatgtcg aagggcgccg tctgttcgcg gaccagccgg tacgaggcgg aaagcgaacg
     4081 gccggttccg gcgagccagc gcgtaccgag ataggaacgc tccatgcgcc cgtcgcgcgg
     4141 gttgtactgc atgccgccgt cgacccagac gctgtcggaa acacgccccg aggcagcgcc
     4201 aaggagatca gtcttgcgct cggttcggcg cgaatcgccc gggctcaacg tgaccgtctg
     4261 cggtgcgaaa tagaagcgct ggccgagcgt gacattgagt cgttcgacgc cgttgtcggg
     4321 ctcaatgaag cgtgacccga cggcgacggt cagctgattg gcatcggcga tacggtcctg
     4381 gccggtaaac aggttttcgg aaaagatctg cgcaaagttg aaatcagcga tgccgctgtc
     4441 gaagttaatc tggttgttgg tgaggaagct ctgatcgcgc agcggcacgt agaggtagaa
     4501 cagcctcggc tcgagcgtct gcgtcagatc cttgtcgaac catcgtgact gtcgctcgaa
     4561 gactagcccg ctgtcgaggc tggcaatcgg cacttcgcgc cggtaggcgt cgggcagacc
     4621 agccgcctcg gcggcatcgc ccgcgaaact gtagcgcgtg aggtgcagcc cgatcttcgg
     4681 cgtgacgtaa ccccagggac gaacgttggg ccaagcgagc tgcggataaa gcacggtgcg
     4741 gcgcgcctcg attcggtccg aatcgggatg ctggaaatcc gtgtattgcg cggtgagcac
     4801 accggtgaga tcgccggcca gcgcctgcca gccggtcaga ttgaactgcg gcatcagctc
     4861 gtagggacgc gccacgaggt tggccggatc gggctgcaac gtctggtagc gcgttgcctt
     4921 gatatcggcg gtccaggcgc cggcgctcca gccgagcagg ccctggcgta ccagctgggt
     4981 ctgcgccgag gtactggcgc ggctcgacag gtcgcggtag ta
//
LOCUS       DATDAA010000395        20564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_448381,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000395 DATDAA010000000
VERSION     DATDAA010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 20564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20564
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448381"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VIS73_11795"
     CDS             <1..461
                     /locus_tag="VIS73_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763880.1"
                     /translation="FRRLLDIGAALAVLRARAGVSPPTVRFIDDWQESSAHAGTTLGR
                     HWIMQVSEHLDRFRQLQVHVKPLSTTMPAHRFTEPHRRRNGPELHQALLAFDRLAGYP
                     MAWFFNMVATHTVPTWVAVNVVEDAGQEFRYLPERDIAVVRNWLHRPYAP"
     gene            477..929
                     /locus_tag="VIS73_11800"
     CDS             477..929
                     /locus_tag="VIS73_11800"
                     /inference="COORDINATES: protein motif:HMM:NF016160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HEY5763881.1"
                     /translation="MTAERIATIRELDDDSVTVEIAMDSACGSCSSRTHCGSKSARII
                     RLPLAACDAFAAGRLPVTGERVRLMSDLPVIRAGLLGYLLPVLALIAGATAGQIALVS
                     TAEANAQPLIADLGAAAGALLGLVGALVALRRRDRGLRLRLAPVESRG"
     gene            complement(918..1379)
                     /locus_tag="VIS73_11805"
     CDS             complement(918..1379)
                     /locus_tag="VIS73_11805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HEY5763882.1"
                     /translation="MAIEKLDASLTLDHAIIDHDHQELFETIQTVNAIIASVNADADD
                     REWSSRVASAIDALRAATIAHFRSEETIMRMGAYPGIDAHRHQHVLLIDELDRFLSGR
                     LVPGPEGSRLAVKFLEEWFEFHIRIWDSALVRWLKGHDGKRAKRSGTGSAS"
     gene            complement(1535..2665)
                     /gene="nifV"
                     /locus_tag="VIS73_11810"
     CDS             complement(1535..2665)
                     /gene="nifV"
                     /locus_tag="VIS73_11810"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HEY5763883.1"
                     /translation="MVAICINDTTLRDGEQTAGVAFSTVEKLAIAEALGNAGVGEIEI
                     GVPAMGSREREVINAICALDLPARTVVWGRMCADDLRDALSCRSDIVHLSTPASELQI
                     RGKLGRDPDWAVANLQAIVRQAVDAGATVSVGAEDASRASLDLLCRLLDAAQEAGAVR
                     FRFADTVGRLDPFETHAVIGALRARSDLELEMHAHDDLGLATANTLAAVRAGASHVNT
                     TVNGLGERAGNAALEEVAMGLRVVHGIDCGVVLTSLREISSLVARAAGRPVADNKSIV
                     GRGVFTHESGIHVDGLLKDPTNYQAFDPAELGRSHRLVLGKHSGSAAVIGAYRRLGID
                     LDEARVHAILEQVRNHSAETKRPPGFRDLMRYYFSTTGAVAH"
     gene            complement(2809..3729)
                     /locus_tag="VIS73_11815"
     CDS             complement(2809..3729)
                     /locus_tag="VIS73_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY5763884.1"
                     /translation="MPATHYHVRHETAYEYTVPITLSRHIAHLEPRMTRWQRIVRHDL
                     EVVPVPGQVIPGVDPFGNPVARISIATPHERLAVVGDTEVAVAPRPWAAANFADYWDW
                     LRTVEGLRYRGGLPVSVTRFRFESPHVRVKRELAALATPSLDTEANLAAACLDLTRRI
                     HSEFAYEPTSTEIGTSVLEVLHTKKGVCQDFAHLMISALRSNGLAARYVSGYLLTEAA
                     PGSERLVGVDASHAWVAVYCPNDVSGGDWIEFDPTNGCLADTRYITLGWGRDFSDVSP
                     LRGVILGGGEHDMSVAVTVSPVDPNGSLRD"
     gene            complement(3762..6260)
                     /locus_tag="VIS73_11820"
     CDS             complement(3762..6260)
                     /locus_tag="VIS73_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY5763885.1"
                     /translation="MATTLLSLYPRPDDRFDELLAPDGSVRVHWQPLIERLEAATPGE
                     MAGWLDFIQGRIQENGVTYNVYADPKGADRPWALDPLPMVLPPQEWSYLEQAVTQRAR
                     LLDAVLGDLYGAQQLLAEGLVPPALVYGHPGFLWPSQGVAPVGGNYLHFYAVDLARSP
                     NGKWWVIADRTQAPSGAGYALENRLVVSRLYPELFQGQRVQHLAGFFRALQENLTAQA
                     PTDPGESPLIVLLTPGPYNETYFEHAYLARYLGFSLVEGQDLTVRGDTLFLKTLTGLK
                     RVHVVLRRLDDDYCDPLELRSESTLGIAGLLQVVRAGRVLIANALGSGVLENPALSGF
                     LPAICESLLGETQMMPSVASWWCGEVAALEYVLDNLDDLVIKPVYPAQRLEPVFGRDL
                     SGAQRADMIARIEASPHAFMAQELVHLSQAPIVSRGVERRLLARSIGLRVFVVAGPDG
                     YRVMPGGLTRVASSSDVPVISMQRGGSSKDTWVISESPVSTFSMLKRRLGAADILRAA
                     PYVPSRSVENLYWLGRYTERSENAARLLRAVINRLTSDDDEDGLRSALEVAERLALIP
                     RSAMSSDPAVARRVLRGIYDADWPGSLVAIGRRVGWAAGHVRERLSADHWHALSRLQD
                     ALRPPIGRPRLDHATSMLDRVVLACTSLAGFVMDDMARDDGWRFLIIGRRIERLQHLS
                     GMVAGFLRQCEGAAGETDCLLEVADGLETYRYRYQRPPELVPVIDLVVCDPDNPHSVM
                     FQIDKLGRYLDALKHEALSAALAPLREAVAVVAKFDLATLEIHSAESGECPACAEFAA
                     DLERIWQCSAMLAEAIGRCAFTHLGPESSRSVGA"
     gene            complement(6298..9615)
                     /locus_tag="VIS73_11825"
     CDS             complement(6298..9615)
                     /locus_tag="VIS73_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY5763886.1"
                     /translation="MSIHVCLNHRTEYAYERAISLGPQVVRLRPAAHSRTRVLSYSLR
                     VTPEDHFINWQQDPHGNWLARLVFPEKTKRFAIEVDVVAEMAVLNPFDFFLEPGVEKF
                     PFAYEKGLRHDLLPFLEKIPATPKFAAYLAEVPREEMRTIDFLVALNQRLCTDIRYLI
                     RLEPGVQTPEETLVKASGSCRDSAWLLVQLLRHLGLAARFVSGYLIQLKADVKSLDGP
                     SGTEVDFTDLHAWCEVYLPGAGWVGFDPTSGLLAGEGHIPLACSPEPSSAAPVSGVVE
                     PCKSELEHHMTVTRVWEAPRVTKPYTPEQWKAIDALGHAVDVRLNAADARLTMGGEPT
                     FISIDDPDGAEWNTAALGPTKRRLAAEVFHRMREHYAPQGLAHFGQGKWYPGEQLPRW
                     SLNCFWRRDGEPIWSDPALYADEAKPSGADAALAGRFLTALAKRLGLNTKHVFPAFED
                     AFYYLWRERQLPVNVDPFDSRLEDKLERARLARVFSKGLDAEVGYVLPIAKEGEEWRS
                     SPWFLRAERCYLHPGDSPVGYRLPIDSQPWVAKADYPWLHVQDPFDARSPLPAKPDLN
                     RQHVTATRPSLTSGASPAAGPAPRADVAPRPGESASTVTRTAMCAEAREGTLYLFMPP
                     LTTADDYLELVAAIEATAETLATPVLLEGYEPPKDPRLNSFRVTPDPGVIEVNIQPAA
                     SWEALSEQTSFLYETARESRLATEKFMVDGRHTGTGGGNHFVLGGATPADSPFLRRPD
                     LLRSLISYWHNHPSLSYLFSGLFIGPTSQAPRVDEARNDALYELELAFKQLPQRGESA
                     PPWLVDRVLRNLLVDVTGNTHRAEFCIDKLYSPDSATGRLGLLEMRAFEMPPHAQMSL
                     AQQLLLRALVARFWEEPYAPPKLVRWGTELHDRFLLPHFVWQDFGDVIDDLRGSGYAF
                     EANWFAPHLSFRFPFVGDFEARGASIEIRQALEPWHVMGEEGAAGGAVRYVDSSVERV
                     QVKADGLSADRYVLTCNGVPVPLQPTGTVGEWVAGVRYRAWQPESCLHPTIGVHAPLT
                     FDLYDTWTQRALGGCRMHVAHPGGRNYVTFPVNAFEAESRRLARFFRHGHTPGGFVPA
                     TPTVNAEHPFTLDLRRP"
     gene            complement(9837..10634)
                     /gene="murI"
                     /locus_tag="VIS73_11830"
     CDS             complement(9837..10634)
                     /gene="murI"
                     /locus_tag="VIS73_11830"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY5763887.1"
                     /translation="MDSTQQAPIGVFDSGVGGLSVLRAIRRELPHENLIYIADSAHAP
                     YGDRDRDYVEARAMALLDYLSKAGAKAVVVACNTATVIAAERLRASYALPIIAMEPAI
                     KPAASCTRSKVIGVLATTQTLASPSVARLCERHGDGISILLQPCPGLVEQIERGNIAG
                     DATRVLLTNYLSPLLAAGADTLVLGCTHYLFLEPLIRELAGPEVAVVDSSAAVARELR
                     RRLGSAVRGVSDGDGCEELLTTAETVVDLQRVIRQLWSAAASVKRLA"
     gene            complement(10785..11528)
                     /locus_tag="VIS73_11835"
     CDS             complement(10785..11528)
                     /locus_tag="VIS73_11835"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEY5763888.1"
                     /translation="MATGFALAASAQAAAGACTAETGASRNTLVELFTSEGCSSCPPA
                     DGWLSALKDRDDLIALAFHVDYWDRLGWTDRFASPRFTQRQHDKASQARSSYVYTPQV
                     LVDGGDFRGWSAYPGGAGLARDQAAVLRLRLAASRAGDVISVKLASEPVREGAWAPRR
                     PSAAWIAVVEDGLASEVAAGENAGKQLRHDRVVRGWHGPFPLDASGRLELARDLSVAG
                     TRPGQSRIVAVVEEAGGGQILQALQLACH"
     gene            complement(11646..12545)
                     /locus_tag="VIS73_11840"
     CDS             complement(11646..12545)
                     /locus_tag="VIS73_11840"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5763889.1"
                     /translation="MAAFVRAIELGGFSAAAREFGVTPSALSKLVARLEDRLGVRLVH
                     RTTRKLSLTPEGETYFARCQRILADIAEAEDEIGRTRRGPTGLLRIGVGTAFGMHQLV
                     PALPRFLERYPGIRLELSVTDRRLDILDEGVDLMIRMGALSDSTLVARKICDFERVVC
                     ASPDYLARHGTPQRPEELLSHNCLYVSTMPELRRWPFRCGEAVTELEVGGIVGSDSAE
                     ALLQLAVEGVGILRLSELIVGAAIARGELVPILADCHQPESLPIYAVYPSARQRSPKV
                     SAMLDFLIEHFAHQPWRSSGDPL"
     gene            12686..12859
                     /locus_tag="VIS73_11845"
     CDS             12686..12859
                     /locus_tag="VIS73_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763890.1"
                     /translation="MERIDYGTYIRRAHQLRTDAIADFGRGIVMALVRAYRSARRSLA
                     AELNRQAPTTRAC"
     gene            complement(12906..13634)
                     /locus_tag="VIS73_11850"
     CDS             complement(12906..13634)
                     /locus_tag="VIS73_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HEY5763891.1"
                     /translation="MTYCVAMRVDAGIVFLSDSRTNAGVDHINSFRKMVYWENVDDRV
                     MVMLTSGNLSISQAVINLINEKISRGDPDSLYAAPNLFEASVLVGAALREVHARDAAA
                     LAASGIDFNAAFIFGGQIQGERPRLFHIYAAGNFIEATDDTPYFQIGESKYGKPIIDR
                     VVKRSTSLAEAAKCALISMDSTIRSNLSVGLPLDLLVMETDALKVRDHQNIESNHAYF
                     QTIRNQWGVRLREAFAELPNPEWL"
     gene            complement(13704..14645)
                     /locus_tag="VIS73_11855"
     CDS             complement(13704..14645)
                     /locus_tag="VIS73_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEY5763892.1"
                     /translation="MLSRTADHLFWMSRYSERAENLARLLDVTYQMSLVPQDERAAQR
                     NWNAVITLNSLEKDFAATGRAPSAQAVLEFMVTDDSNPASIRTCLRLARENAHAVRGA
                     MTAEMWETMNATWLELRRKSFDEIEAGGIAEFFEWVKERASLFRGATFGTMLQDEAFH
                     FIRIGVLLERADNTARLLDVKYHGLAARDAEGTARDFYQWAAVLRSLSAFEVYRKVFR
                     DAITPARVAELLIFNEDLPRSLHACIKGVLERLPKIANDRSKETERRIGMLHAMLRYG
                     RIEDVLANGLHPYLEHFMDSVYDIGDGISSDFLLGRN"
     gene            complement(14638..16062)
                     /locus_tag="VIS73_11860"
     CDS             complement(14638..16062)
                     /locus_tag="VIS73_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY5763893.1"
                     /translation="MPNKFFDEMTDSSGAIRSHYESFENWLANMASERLKRKRAEADL
                     TFHRMGITFAVYGEESGKERLIPFDIVPRIIPAHEWATLQNGLKQRVRALNMFLHDIY
                     HDQKILKSGIVPSEEVLGNAQYRKEMQGVDVAADIYAQIAGVDIVRAGEGEFYVLEDN
                     LRVPSGVSYMLEDRRMMMRLFPELFAMQRVAPVDHYPDLLLDTLRAVAPDAIDDPTVV
                     VMSPGAYNSAYFEHAFLAQQMGVELVEGRDLFVDHDTVYMRTTRGPTKVDVIYRRIDD
                     DFLDPKSFRADSMIGVAGLFDAYRAGRVTLCNAIGTGVADDKSIYPYVPEIIRYFLDE
                     EPILNNVPTWRCREAKSLEHVLANLESLVVKEVHGAGGYGMLVGPAASKKEVEDFRAH
                     LIANPGKYIAQPTLALSTCPTFVDAGIAPRHIDLRPFVLSGKDVNLVPGGLTRVALRE
                     GSLVVNSSQGGGTKDTWVLENGHA"
     gene            complement(16247..17335)
                     /gene="modC"
                     /locus_tag="VIS73_11865"
     CDS             complement(16247..17335)
                     /gene="modC"
                     /locus_tag="VIS73_11865"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEY5763894.1"
                     /translation="MTAVAASGADAGITACFKLAYPGFSLDVDLTLPGHGITAFFGHS
                     GSGKTTLLRAIAGLERVDDGRLVVGGEVWQDATGCLPTHARPLGYVFQEASLFPHLSV
                     RANIEYGLRRAGAAESGFDAIVDLLGISPLLGRKPERLSGGERSRVAIARALVTSPRL
                     LLMDEPLAALDAARKAEFMPYLERLHRELNIPVLYVTHAIDEVARLADHLVLLADGRV
                     QASGPTAATLTRTDLPLARGDEASAIIDGTVAVQDDVYRLTRIDCAAGPVWVSQLALP
                     IGTAVRARVLARDVSIAIEKARETSILNLIPARVEAVVPDGPGRALVTVNAKGVYLLS
                     RITERSVALLGVRPGREVVAQIKGVAVL"
     gene            complement(17332..18006)
                     /gene="modB"
                     /locus_tag="VIS73_11870"
     CDS             complement(17332..18006)
                     /gene="modB"
                     /locus_tag="VIS73_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEY5763895.1"
                     /translation="MLSPDDLAAIWLTLKLATVTTVVLLVIGTPIAWWLARTRSAWRG
                     PVGALVAMPIVLPPTVIGFYLLLLMGPNGPVGQFTQWAGIGLLPFTFWGLVVASVFYS
                     MPFVVQPIQNAFEAIGERPLEVAATLRARPLDAFFSVAVPLARPGFLTAAILGFAHTV
                     GEFGVVLMIGGNIPGATRVVSVQIYDHVEAMEYAQAHWLAAGMIVFSFLVLLALYSLA
                     PTRKRS"
     gene            complement(18062..18448)
                     /locus_tag="VIS73_11875"
     CDS             complement(18062..18448)
                     /locus_tag="VIS73_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763896.1"
                     /translation="MKIAIAVKPDLRTVSGHAGQTPDWVVFDCQPGMPPPVPQAICLS
                     RAQLPHYFRDDGPHPLHGVDVFVAASAGEGFVRHMQQWGASVLLTGERDADVALATIL
                     RGESLADPRFDMTAALCRLRDLFSRH"
     gene            complement(18469..19149)
                     /gene="modA"
                     /locus_tag="VIS73_11880"
     CDS             complement(18469..19149)
                     /gene="modA"
                     /locus_tag="VIS73_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5763897.1"
                     /translation="MAVASNFARPAKVLVEEFEQKTGHRATIATGSTGKFYAQIRNGA
                     PFDVLLSADSETPRRLVDERLGLAGSRMTYAIGRLVLWSARAGVVDPQGAVLRQGQFR
                     KLAIANPKLAPYGAAAREVLGKLNVLDIATPRLVQAENIAQAHQFVASGNAELGFVAL
                     SQVIVDGRVGAGSGWVVPSELHSPIRQDAVMVTEAKNNRAAAAFMDFLSTEVARRIIR
                     NHGYEVVP"
     gene            complement(19256..19726)
                     /locus_tag="VIS73_11885"
     CDS             complement(19256..19726)
                     /locus_tag="VIS73_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifQ"
                     /protein_id="HEY5763898.1"
                     /translation="MHRARSDSDAGADLRLIDHRWRAGGEMAEAGERNEEFEDLRDLL
                     LDGAINAADPAVAELAAAMARACLGHDHLWHDLGLATRDELSRILYTHFPNLAALNVG
                     RMRWKKFFYKQLCERLDVRACRAPSCGVCANQAECFGPEDVPIKRMSWSLAVSL"
     gene            complement(19707..20009)
                     /gene="fdxB"
                     /locus_tag="VIS73_11890"
     CDS             complement(19707..20009)
                     /gene="fdxB"
                     /locus_tag="VIS73_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin III, nif-specific"
                     /protein_id="HEY5763899.1"
                     /translation="MSTFTVTLPSGVEWTPHFATGVDPAKCIGCGRCFKTCAREVLEL
                     MGVDDDGEYVALDDDDDADEYEKKVMTVGHPENCVGCESCGKVCGKKAISHAPRAL"
     gene            complement(20038..20244)
                     /locus_tag="VIS73_11895"
     CDS             complement(20038..20244)
                     /locus_tag="VIS73_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCE_0567 family metalloprotein"
                     /protein_id="HEY5763900.1"
                     /translation="MGDEKSIDMRKLSARATQAKMDLHDLSEELPLGWERIMEQAQKT
                     YDAFAALAAAKSEAAVLAPAKASS"
     gene            complement(20260..>20564)
                     /locus_tag="VIS73_11900"
     CDS             complement(20260..>20564)
                     /locus_tag="VIS73_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NifX-associated nitrogen fixation protein"
                     /protein_id="HEY5763901.1"
                     /translation="MPIIDDPDPDVLWRLELYYNAIGLSIERATGIMCAPMIKINHEG
                     FGRAVLLAGRVVAYSKHHRDVHRFGFDSLEKLAEGGDRVVAEAGNLIQRYRDLAEM"
BASE COUNT         3771 a         6681 c         6669 g         3443 t
ORIGIN      
        1 ccttccggcg gctgctcgac atcggcgccg cactcgccgt actgcgagcc cgcgccgggg
       61 tatcgccacc gaccgtgcgt ttcatcgacg attggcagga aagcagcgcg cacgccggca
      121 cgacgctcgg gcgacattgg atcatgcagg tcagcgaaca ccttgaccgc ttccgacaac
      181 tccaggtaca cgtgaaaccg ctcagcacca ccatgccggc acatcgcttt accgagcccc
      241 accggcggcg caacggcccg gagttgcatc aggcgctgct cgccttcgac cgcctggctg
      301 gatatccgat ggcctggttc ttcaacatgg tggcgacgca cacggtcccg acgtgggttg
      361 ccgtcaacgt ggtcgaggac gccggccagg agtttcgcta cctccccgag cgcgacatcg
      421 ccgtggtgcg taactggctt caccgaccgt acgcgccgta ggctggattt ccgacgatga
      481 cggccgaacg catagcaaca attcgcgaac tcgatgacga ctcggtcacc gtggaaatcg
      541 ccatggacag cgcctgcggg tcctgttcga gccgcacgca ttgcggcagc aaaagcgccc
      601 ggatcattcg gctgcccctg gcggcatgtg acgccttcgc cgctggccgg ctaccggtaa
      661 ccggcgagcg cgttcgattg atgagcgacc tgccagtaat ccgtgcgggc ctgcttggct
      721 atctgctacc ggtgttggct ttgatcgcgg gcgccaccgc aggtcaaatt gcgctggtgt
      781 ccacagccga ggctaacgcg cagccgctga ttgcagacct gggcgccgcc gccggcgccc
      841 tgcttggcct ggtaggggca ttggttgcgc ttcgccggcg cgaccgcggg cttcgcctgc
      901 gcctcgcacc ggtcgaatca cgaggctgaa ccagtacccg atcgcttcgc gcgcttgccg
      961 tcgtgcccct tgagccagcg caccaacgcg ctgtcccaga tgcgaatgtg aaactcgaac
     1021 cattcttcga gaaacttcac cgcgagccga ctgccttcgg ggcctgggac gagccggccg
     1081 gacaggaacc tgtcgagttc gtctatcagc agcacgtgct gatggcggtg cgcgtcgatc
     1141 cccggatagg cacccatgcg catgatcgtt tcttccgagc ggaaatgcgc gatcgtggcg
     1201 gcgcgtagcg catcgatcgc acttgccacc cggctactcc actcacgatc atccgcgtcg
     1261 gcattcaccg acgcgatgat cgcgttgact gtctggatcg tctcgaacag ttcctgatgg
     1321 tcatgatcga tgattgcgtg atccagcgtc agcgaagcat ccagtttctc gatagccacg
     1381 ttcccccctt ttttcgaaga agtcaggcgc cgtcatgcgc ggttcaagcc accgcgtcta
     1441 tcctgtccgg caccgcggtt gtaatgatat gtcttcggtg accagccgtc gcgggagggt
     1501 ttcgctgaac ggtcaaacgg gccacatggc ctgctcagtg cgcgaccgcg ccggttgtcg
     1561 agaaatagta acgcatcagg tcgcgaaacc ctggtggtcg cttggtttcc gcgctgtggt
     1621 tgcgcacctg ttcaaggatc gcatgcaccc gcgcctcgtc aagatcgatg cccaatcggc
     1681 gatacgcgcc gatgaccgcg gccgagcccg agtgcttgcc gagaaccagg cggtgactgc
     1741 gacccagttc ggcgggatcg aaggcctgat agttcgtcgg gtcctttaac agcccgtcga
     1801 cgtgaatgcc agattcatgc gtaaaaacac cacgcccgac aatgctcttg ttgtcggcca
     1861 ccggccggcc ggcggcgcgc gcgacgagcg acgaaatctc gcgtagcgag gtcagcacca
     1921 ccccgcaatc gattccgtgg acgacacgca gccccatcgc gacttcctcg agcgccgcgt
     1981 tgcccgcgcg ctcgccgagg ccgttgaccg tcgtgttgac gtggctggca ccggcgcgaa
     2041 cggccgccag cgtattggcc gttgccaggc cgaggtcgtc gtgcgcgtgc atttccagtt
     2101 cgaggtcgct gcgggcgcgc agcgcgccga tcaccgcgtg cgtctcaaac ggatcaaggc
     2161 ggcctaccgt gtcggcgaag cggaaacgca cggcgcccgc ttcctgcgcg gcatcgagca
     2221 ggcggcaaag caggtcaagc gaggcgcgcg aggcgtcctc ggcgccaacg ctgaccgtcg
     2281 cgccggcgtc cacggcctgg cggacgatcg cctgcaggtt ggcgaccgcc caatccggat
     2341 cgcggccgag cttgccgcga atctgcagtt ccgaggccgg cgtcgacaag tgcacgatgt
     2401 cagaccggca ggacagcgca tcgcgcaagt catcggcgca catccggccc cagacgacgg
     2461 tacgcgccgg caagtcgagc gcgcagatcg cgttgatcac ttcgcgctcg cgcgacccca
     2521 ttgccggaac gccgatctcg atttcgccga cgccggcgtt gccgagcgcc tcggcgatcg
     2581 ccagcttctc gacggtgctg aaggcgacgc cggcggtttg ctcgccgtca cgcaaggttg
     2641 tgtcgttgat gcagatggcg accatgtcag gctccggaca gggaaatccc ctcatgcgga
     2701 gaactgcaat ttccctgcca gccgtcacgc catgcgcctt ggccgccgac gccttcggtg
     2761 cgcaatggct cggccccgac ggcgtgtcgg aatcacgaca aacctgggtc agtcgcggag
     2821 ggaaccgttc ggatcaaccg gggaaacggt aaccgcgacg ctcatgtcgt gttcgccgcc
     2881 gccgaggatc acgccgcgca acggggatac gtcactgaaa tcgcggcccc agccgagcgt
     2941 gatgtagcgg gtatcggcca ggcaaccgtt ggttggatcg aattctatcc agtcgccgcc
     3001 gctcacatcg ttcgggcaat agaccgcgac ccaggcatgc gaggcatcaa caccgaccag
     3061 acgttcgctg ccaggagctg cttcggtcag caggtagccc gagacgtagc gcgctgcgag
     3121 gccattcgag cgcagcgccg agatcatgag gtgcgcgaaa tcctggcaga cacccttttt
     3181 tgtatgcagt acttcaagga ccgaggtgcc gatctcggta ctcgttggct cgtacgcaaa
     3241 ttcggaatgg atacgacgag tcagatccag gcaggccgcg gcgaggttcg cctcggtgtc
     3301 gagggatggc gtagcgagcg cggccagctc gcgcttgacg cgcacgtgcg gcgactcgaa
     3361 gcggaagcgg gtcaccgaca ccggcagtcc gccgcgatag cgcaatcctt cgaccgtgcg
     3421 cagccagtcc cagtagtcgg cgaagttcgc agcagcccag ggccgcggcg cgacagccac
     3481 ctcggtgtcg ccaacgacgg cgagccgctc atgcggggtg gcgatcgaaa tccgtgcgac
     3541 cggattaccg aagggatcaa ccccggggat cacctgcccc ggaaccggca cgacctcgag
     3601 atcgtgccgg acgatgcgct gccagcgcgt cattcgtggc tcaaggtggg caatgtggcg
     3661 cgacagcgtg atcggcaccg tgtattcgta ggccgtttcg tggcgtacgt gataatgcgt
     3721 cgccggcacg ccgttctcct tcgatgctgg agcggcggcg ttcatgcgcc gaccgagcgt
     3781 gaagactcgg gacccaggtg ggtgaaggca caacggccga ttgcttcggc cagcatagcc
     3841 gagcactgcc agatccgttc gaggtcggcg gcgaactcgg cacaggcagg acactcgccg
     3901 gactcggccg aatggatctc gagcgtggcg aggtcgaact tggcgaccac ggccacggct
     3961 tcgcgcagcg gcgccagcgc ggcgctcaag gcctcgtgtt tcagggcgtc gagatagcgt
     4021 ccgagcttgt cgatctggaa catcaccgaa tgggggttgt ccggatcgca gaccaccagg
     4081 tcgatgacgg gcaccagctc gggcggacgc tgatagcggt aacgataggt ttcgagtccg
     4141 tcggcgactt cgaggagaca gtcggtttcg cccgccgcgc cttcgcactg ccggaggaat
     4201 cccgcaacca tgccgctcag atgctgcagg cgctcgatgc gccgaccgat gatcaggaaa
     4261 cgccagccgt catcgcgcgc catgtcgtcc atcacgaagc cggcgagaga ggtacacgcc
     4321 agcaccacgc ggtcgagcat gctcgtcgcg tggtcgagac gcggccggcc gattgggggc
     4381 cgcagcgcat cctgcagccg tgacagcgca tgccagtggt cggccgacag gcgctcgcgc
     4441 acgtggcctg ctgcccagcc gacgcgccgc ccgatggcga ccagactgcc aggccagtcc
     4501 gcatcgtaga ttccacgcag cacgcgacgg gcaaccgccg gatcgctgct catcgcactg
     4561 cgcgggatca atgccagccg ctcggcaacc tccagcgcgc tgcgcaatcc gtcttcgtcg
     4621 tcgtcgctgg tcaggcgatt gatgactgcg cgcagcagtc gcgcggcgtt ctcactgcgt
     4681 tctgtgtaac ggcccagcca atagaggttc tcgacgctgc ggctcggcac gtacggtgcc
     4741 gcgcgtagga tgtcggccgc gccgaggcgg cgcttgagca tgctgaaggt actgaccggc
     4801 gactcggaga tgacccaggt gtccttgctg ctgccgccgc gctgcatcga gatgaccggc
     4861 acgtcgctcg aactggcaac gcgcgtcagg ccaccgggca tcacgcggta gccgtctggc
     4921 ccggccacga cgaagacgcg caggcctatg ctgcgggcaa gcaggcgccg ctcaacgccg
     4981 cggctgacga tcggcgcctg cgagaggtgc accagttcct gcgccatgaa ggcatgcggg
     5041 ctggcctcga tgcgggcgat catgtcggcg cgctgagccc cggacagatc ccgcccgaag
     5101 acgggctcca ggcgttgcgc gggatagacg ggcttgatca ccaggtcgtc gagattgtcg
     5161 agcacgtatt caagcgccgc cacttcgccg caccaccaag atgccaccga gggcatcatc
     5221 tgcgtttcgc cgagcagcga ttcgcagatc gccggcagga atccgctgag cgccggattc
     5281 tcgaggactc cgctgccgag cgcgttggcg atcagcacgc ggccggcacg aaccacctgc
     5341 agcaggccgg cgatccccag cgtcgattcg gagcgcaatt cgagtggatc gcaatagtcg
     5401 tcatcgagac gccgcaacac gacgtggacg cgtttcagtc cggtcagagt cttcaggaac
     5461 agcgtatcgc cgcgcaccgt caggtcttgg ccctcgacca gagaaaaccc gaggtagcgc
     5521 gccagatacg catgctcgaa ataggtttcg ttgtacggcc ccggcgtcag caacacgatc
     5581 agcggcgact cacccgggtc ggtcggcgcc tgcgccgtga ggttttcctg cagggcgcgg
     5641 aagaaaccgg cgaggtgctg gacgcgctgc ccctggaaca gctcggggta taggcgcgac
     5701 accaccagac ggttctccaa tgcgtaacca gcgcccgatg gcgcctgcgt ccgatcggcg
     5761 atgacccacc atttgccgtt cggcgagcgc gccagatcga ccgcgtagaa gtgcaggtaa
     5821 ttgccgccga ccggtgcaac cccctggctc ggccagagga agcctgggtg gccgtagacc
     5881 agcgccggtg gcaccagccc ctcggcgagc aactgctgtg caccgtagag gtcgccgaga
     5941 actgcatcga gcaggcgcgc gcgttgcgta accgcctgct cgaggtagct ccattcctgc
     6001 ggcggcagga ccatcggcag cggatcgagt gcccagggac ggtcggctcc cttgggatca
     6061 gcgtagacgt tgtaggtaac gccgttttcc tggatccggc cctggatgaa atcgagccag
     6121 ccggccatct cgccgggtgt cgctgcctct aagcgctcta tcagcggctg ccagtgcacc
     6181 cgaacgctgc cgtcgggcgc aagcaactcg tcgaaacggt cgtccgggcg tgggtacagc
     6241 gatagcagcg tggtcgccat ctaatcggtg cgcggccggg ccgccctcct ctgctgctca
     6301 gggacggcgc aagtctaacg tgaatggatg ctcggcattg accgtcggcg tcgccggtac
     6361 gaagccgccc ggtgtgtgcc catgccggaa aaagcgcgcc agacgtcggc tctcggcctc
     6421 gaaggcattg accgggaagg tgacgtagtt acggccgccg gggtgcgcaa cgtgcattcg
     6481 acagccgccg agcgcgcgtt gcgtccaagt gtcgtacagg tcgaaagtca agggcgcgtg
     6541 cacgccaatc gtcgggtgca ggcacgactc cggctgccag gcgcgataac gcacgccggc
     6601 gacccactcg ccgaccgtgc cggtcggctg cagcggcacc ggaacgccat tgcaggtgag
     6661 cacgtagcga tcggccgaca ggccatcggc cttgacctgg acgcgctcga ccgaggaatc
     6721 gacgtagcgc accgcgccac cggcggcacc ctcctcgccc atcacatgcc agggctcgag
     6781 cgcctggcga atctcgatgc tcgcaccgcg cgcctcgaaa tcgccgacga aggggaagcg
     6841 gaacgacagg tgtggcgcga accagttggc ttcgaaggca tagccgctac cgcgcaggtc
     6901 gtcgatgacg tcgccgaagt cctgccagac gaaatgcggc agcaggaagc ggtcgtgcag
     6961 ttcggttccc cagcgcacca gcttcggcgg cgcgtagggt tcttcccaaa agcgcgcaac
     7021 cagcgctcgc agcagcaact gctgggcgag gctcatctgt gcgtgcggcg gcatttcgaa
     7081 ggcgcgcatc tcgagcaggc cgagtcgccc cgtcgcgctg tcaggggaat acagcttgtc
     7141 gatgcagaat tcggcgcggt gcgtgttgcc ggtgacgtcg acgagcaggt tgcgcagcac
     7201 gcggtcaacc agccacggtg gcgcgctctc gccccgctgc ggcaactgct tgaaggcgag
     7261 ttcaagctcg tagagcgcgt cgttgcgcgc ctcgtcaacg cgcggcgcct gcgaggtcgg
     7321 gccaataaag aggccggaga acaggtacga aaggctcggg tggttgtgcc agtacgaaat
     7381 caggctgcgc agcagatcgg gccgcctgag gaaaggcgaa tcggccggag tcgccccgcc
     7441 cagcacgaag tggttgccgc cgccggtgcc ggtgtggcga ccgtcgacca tgaatttctc
     7501 ggtcgccagc cgcgactcgc gcgccgtctc gtaaaggaag gaggtctgct cgctgagcgc
     7561 ttcccagctc gccgccggct ggatgttcac ttcgatcacg ccggggtcgg gtgtcacccg
     7621 gaaactattc agtcgcgggt ccttcggcgg ctcgtatcct tcgagcaaca ccggcgtggc
     7681 caacgtttcg gcggtcgcct cgattgccgc gaccagttcg agatagtcgt cggccgtcgt
     7741 cagcggcggc atgaacaggt agagcgtacc ctcgcgcgcc tcagcgcaca tcgccgtgcg
     7801 cgtcaccgtt gacgctgact cgccggggcg cggcgcgacg tcggcgcgcg gcgccgggcc
     7861 ggcggccgga ctcgcgcctg atgtcaggct gggccgcgtg gcggtgacgt gctgccggtt
     7921 caggtccggc tttgccggca gcggcgaacg cgcgtcgaac gggtcctgca cgtgcaacca
     7981 cgggtaatcg gccttggcaa cccagggctg tgagtcgatc ggcagacggt agcccaccgg
     8041 cgaatcaccg ggatgcagat agcagcgctc ggcgcgcagg aaccaggggc tgcttcgcca
     8101 ttcctcacct tctttggcta tcggcaacac gtagccgacc tcggcatcga gacccttcga
     8161 gaagacgcgc gccagacgcg cgcgctcgag cttgtcctcg agccggctgt cgaatggatc
     8221 gacgttgacc ggcaattgcc gctcgcgcca gagatagtaa aaagcgtcct cgaaagccgg
     8281 aaagacatgc ttcgtgttga ggccgagacg cttggcgagc gccgtcagga agcgccccgc
     8341 cagcgccgca tcggcaccgc tcggttttgc ctcgtcggcg tagagcgccg gatcgctcca
     8401 gatcggctcg ccgtcgcgac gccagaaaca gttgaggctc cagcgcggca actgctcgcc
     8461 ggggtaccac ttgccctggc cgaagtgcgc gagaccctgc ggtgcgtaat gctcacgcat
     8521 ccggtggaag acctcggccg caagacgccg cttggtcggc cccagggccg cggtgttcca
     8581 ctcggcgccg tcgggatcgt cgatcgaaat gaaagtcggc tcgccaccca tcgtcagccg
     8641 tgcgtcggcc gcgttcagcc ggacgtcgac cgcatgcccg agcgcgtcga tcgctttcca
     8701 ctgctcgggc gtatacggct tggtcacgcg cggcgcttcc cagactcggg tgaccgtcat
     8761 gtggtgctcg agctcgctct tgcagggttc gaccacgccg ctgaccggcg ccgccgacga
     8821 cggctcgggc gagcaggcga gcggaatatg cccctcgccg gcgagaagac ccgacgttgg
     8881 gtcgaagccg acccaccctg cgcccggcag gtagacctcg caccaggcgt gcaggtcggt
     8941 gaaatcgacc tcggtgcccg acggaccgtc gagcgacttg acgtcggcct tgagctggat
     9001 caggtagccc gagacgaagc gcgccgccag accaagatgc cgcagcagct gcaccagcag
     9061 ccaggccgag tcgcggcacg acccggaggc cttgaccagc gtctcctcgg gcgtctgcac
     9121 gccgggttca aggcggatca ggtagcggat atcggtacat agccgttgat tcagcgcgac
     9181 caggaaatcg atcgtgcgca tttcctcgcg cggcacttcg gcaagatacg cagcgaactt
     9241 tggcgttgcg ggaatttttt cgagaaacgg caacaggtca tggcgcagcc ccttttcgta
     9301 ggcaaaagga aacttctcga cgccgggctc gaggaagaaa tcgaaggggt tgagcacggc
     9361 catctcggcc accacgtcta cctcgatcgc gaagcgcttg gtcttctcgg ggaaaaccag
     9421 tcgcgccagc cagttgccgt gcgggtcctg ctgccagtta atgaaatggt cttccggcgt
     9481 aacccgaagt gaataggaga gtacgcgagt gcgcgaatgc gccgccgggc gcaggcgcac
     9541 gacctgaggg ccgagcgaaa tcgcgcgttc gtaggcgtat tcggtacggt ggttcagaca
     9601 gacgtgtatg gacacaaacg ttctccgctc agggcgacct tggatgagct agcaagacgc
     9661 gaaccatcat cgcgacggaa gaaacgccgc gccataacgg cgaataggat gcagtctgga
     9721 aagcggacgc accaataggc gtcggcagtc gcaactgcgc accagtttaa cgcatcgatt
     9781 tcacgcgcca gccgaggagg cgtgtcggcc gatcggtcgg ctgaacgagg gcagctctac
     9841 gccaatcgtt ttaccgatgc cgcggcgctc cacaactgcc tgatgacccg ttgaagatcg
     9901 acgaccgtct ccgccgtggt cagaagctcc tcgcatccgt ctccgtcgct gacgccacgg
     9961 accgcgctgc cgagtcgccg ccgcagttcc cgcgcgaccg ccgctgagga gtcgacaact
    10021 gccacctctg gcccggccag ctcacgaatc agcggctcga gaaaaaggta gtgggtgcag
    10081 ccgagcacga gcgtatcggc gccggcagcc agcagcggcg acaggtagtt tgtcagcaac
    10141 acgcgagtcg catcaccagc aatgttgccg cgctcgattt gctcgaccag gccggggcag
    10201 ggctgcaaca gaatgctgat tccgtcgccg tggcgttcgc acaatcgtgc gacggagggg
    10261 ctcgctaggg tctgcgtggt ggcgaggacg ccgattacct tcgaacgcgt gcagctggcg
    10321 gccggcttga tcgctggctc catcgcgatg atcggcagcg cgtagctggc gcgcagccgc
    10381 tcggcggcga tcacggtggc ggtgttgcag gcaacgacga cggccttggc gcctgccttg
    10441 gaaagataat caagcagcgc catcgcccgc gcctcaacgt agtctcgatc acgatcgccg
    10501 taaggggcgt gcgccgagtc ggcgatatag atcaggttct cgtgcggcag ctcgcgacgg
    10561 atcgcccgca acaccgaaag gccgccgacg ccggaatcga agacgccgat tggcgcctgc
    10621 tgcgtggaat ccattttgta gcgcggtggt accgatccgt cgggaagaag tcagcgccga
    10681 cgggcgtcgg cggcatgtcg cgccgagttg cccggcgacg cgcggcaatc tacgccttcg
    10741 gcccacggtg tcaatggtgc ccgactcgct gcgctgcgtg gcgctcaatg gcaagcgagc
    10801 tggagcgcct gcaggatctg gccgccaccc gcctcctcga ccacggcgac gatacggctc
    10861 tgcccgggcc gcgtgccagc gaccgacagg tcgcgcgcca gctcgagtcg cccgctggca
    10921 tccagcggaa acggcccgtg ccagccgcgg acgacgcgat cgtggcgaag ctgcttgccg
    10981 gcgttctcgc cggccgccac ctcgctggcg aggccgtcct cgaccacggc tatccaggcg
    11041 gcgctcggcc gccgtggcgc ccatgcccct tcccgcacag gttcgctcgc cagtttgacc
    11101 gagatgacgt cgccggcacg cgaagcggcc agccgcaggc gcaggaccgc ggcttgatcc
    11161 cgcgccagtc cggcaccgcc tggatacgcg gaccacccgc ggaaatcacc gccatcgacg
    11221 agcacctgcg gcgtataaac atagctgctg cgagcctggc tcgccttgtc gtgctggcgc
    11281 tgggtaaagc gaggcgaggc gaagcggtcg gtccagccca gccgatccca gtagtcgacg
    11341 tggaaggcca gcgcaatcag gtcgtcgcgg tctttcagcg cgctcaacca accgtccgcc
    11401 ggcgggcagc tgctgcagcc ctcggaggta aacagctcga ccagcgtgtt gcgtgaggcc
    11461 ccggtttcgg ccgtgcaggc gcccgccgcg gcctgcgccg acgcggccag tgcgaaaccg
    11521 gtggccatcg cgagaaacgc tcggcacccg ccgccatggc cgcgccgcgc ttcctgaaat
    11581 ctcgtttgat ccatccgggt ccccgtttct ggaatgaccg tcggtcggcg gcgggccttc
    11641 attccttaca gcggatcgcc gctgcttcgc cagggctggt gcgcgaaatg ctcgatgagg
    11701 aaatcgagca tcgccgaaac cttgggcgaa cgctgccgcg ccgatgggta gacggcgtag
    11761 atcggcagcg attcgggctg atggcaatcg gcgaggatgg gcaccagctc gccacgcgcg
    11821 atcgccgcgc cgacgatcag ctcgctcagg cggaggatgc cgacgccttc gacagccagt
    11881 tgcagaaggg cctcggcact gtcggagccg acgattcccc caacctcgag ctcggtcacc
    11941 gcctcaccac agcgaaacgg ccagcgtcgg agttcgggca tggtcgagac atagaggcag
    12001 ttgtgcgaga gcagctcctc gggacgctgt ggcgtgccgt ggcgtgcgag gtaatcaggt
    12061 gatgcgcaga cgacgcgctc gaagtcgcag atcttgcggg cgaccagcgt cgaatcgctc
    12121 aatgcgccca tgcggatcat caggtctacg ccttcgtcga ggatatcgag tcggcggtcg
    12181 gtgaccgata gctcgaggcg gattcccgga tagcgctcaa ggaagcgcgg cagcgccggt
    12241 acgagctggt gcatgccgaa agccgtgccg acgccgattc gcagcaagcc ggtcggcccg
    12301 cggcgggtgc ggccgatttc gtcctcggct tcggcgatgt cggcaaggat gcgctggcag
    12361 cgggcaaagt aggtctcgcc ctcgggtgtc agcgacagct tgcgcgtcgt ccgatgcacc
    12421 agccggaccc ctaggcgatc ttcgaggcgg gccacaagct tggacagcgc cgacggcgtc
    12481 accccgaatt cgcgtgcggc ggcggaaaag ccaccgagtt cgatggcgcg gacaaaggcg
    12541 gccatttcac caccgctgca acggtttatt tgtgccatag cagcacagat gttattccat
    12601 ctttgctaat tgtttctttt tcggcagcaa ttatagtgat cgcacggtga cggccagtga
    12661 ggccgaccga gatcgggaga tgagcatgga acggatcgac tacggaacct acatacgccg
    12721 ggcccaccag ttgcggaccg acgccatcgc cgacttcggc cgggggatcg tcatggcgct
    12781 ggtcagggct tatcgcagcg cccgtcgttc gctcgccgca gaactgaatc gccaggcacc
    12841 caccacccgg gcctgctgag caaaggggcc gaccgcccgg cgccgcccta acccgcgcgt
    12901 tctaactaga gccattcggg attgggaagt tcggcaaacg cctcgcgcag gcgcacgccc
    12961 cactggttgc ggatggtctg gaaataggcg tggttagact cgatgttctg gtgatcgcga
    13021 accttcagcg cgtcggtctc catcaccagc agatcgagtg gcagaccaac cgagagattg
    13081 gagcgaatcg tcgaatccat cgaaatcagc gcgcacttgg ccgcctcggc caaagaggta
    13141 ctgcgcttga cgacgcgatc gatgattggt ttgccgtatt tcgactcgcc aatctggaaa
    13201 tatggcgtgt cgtcggttgc ctcgataaag ttgcctgccg cataaatgtg gaacaaccgc
    13261 ggccgctccc cctggatctg gccaccgaag ataaaggctg cgttgaaatc gattcctgat
    13321 gccgccagcg ccgccgcatc gcgtgcatgc acttcgcgca gcgcagcgcc gacaagtacc
    13381 gatgcctcga acaggttggg cgctgcgtac aggctatccg gatcgccgcg gctgatcttc
    13441 tcgttgatca ggttgatgac cgcctggctg atcgagagat tgcccgaggt cagcatgacc
    13501 atgacccggt catcgacgtt ctcccaatac accatcttgc gaaacgagtt gatgtgatca
    13561 accccggcgt tggttcggga gtccgacagg aagacgatgc cggcatcgac gcgcatcgcg
    13621 acgcaatagg tcatgaggaa gattccgcca gaaaagcgcg cagtctaccg gcccgccgcc
    13681 ggaccggtaa ctgggttcag cgattaattt cggccgagca ggaagtcgga gctgatgccg
    13741 tcgccgatgt cgtacaccga atccatgaag tgctcgagat acgggtgcag gccattggcc
    13801 agaacgtcct cgatgcggcc gtagcgcaac atggcgtgga gcatgccgat gcgccgttcg
    13861 gtctccttcg accggtcgtt ggcgatcttg ggcaatcgct ctagcacacc cttaatgcag
    13921 gcgtgcaggc tgcgcggcag atcttcgttg aaaatcagca gttcggccac gcgtgccggg
    13981 gtaatggcgt cgcggaagac cttgcgataa acctcgaagg ccgacagcga gcgtaggaca
    14041 gcggcccact ggtagaagtc ccgggcagtt ccttcggcat cgcgtgcggc caagccgtgg
    14101 tacttcacat cgagcagccg cgcggtgtta tcggcgcgct cgagcagcac gccgatgcgg
    14161 atgaagtgga aggcctcgtc ctgcagcatg gtaccgaagg tggcgccacg gaaaagcgat
    14221 gcgcgttcct tcacccactc aaagaactct gcgatgccgc ccgcctcgat ttcatcgaac
    14281 gacttgcggc gcagttcgag ccaggtcgcg ttcatcgttt cccacatctc ggccgtcatt
    14341 gcgccacgta cggcatgagc gttctcacgc gcaaggcgca gacaggtccg gatcgatgcg
    14401 ggattgctgt cgtcggtgac catgaattcc agaaccgctt gggcactcgg cgctcgaccg
    14461 gttgcagcga agtccttctc caggctgttc agcgtgatga cagcgttcca gttgcgttgc
    14521 gccgcacgct catcctgcgg cacaagcgac atctggtagg tgacgtcgag caggcgcgcg
    14581 agattctcgg cgcgctcgga gtagcgggac atccagaaca ggtgatccgc agttcgacta
    14641 agcatgaccg ttctccagca cccaggtgtc tttcgtgccc ccaccctggg atgagttgac
    14701 caccagcgag ccctcgcgaa gcgccacgcg ggtcaggcca ccgggaacga gattgacgtc
    14761 cttgccagag agcacaaagg gccgcaggtc gatatgccgc ggcgcgattc ccgcgtcgac
    14821 gaaggtcggg caggtcgaca gcgccagcgt cggctgggcg atgtactttc cggggttggc
    14881 gattaggtgt gcgcggaagt cctcgacttc cttcttgctc gccgccggcc cgaccagcat
    14941 gccgtagccg cccgcaccat ggacttcctt gacgaccagt gactcgaggt tggccaatac
    15001 atgctcgagc gatttcgcct cgcggcagcg ccaggtcggc acgttgttga ggatgggttc
    15061 ttcgtcaagg aagtagcgaa tgatctcggg aacgtagggg tagatcgact tgtcatcggc
    15121 aacgccggtg ccgatcgcgt tgcaaagcgt gacccggccg gcgcggtagg cgtcgaaaag
    15181 cccggcgacg ccgatcatcg aatcggcgcg gaaggatttt ggatcgagga agtcatcgtc
    15241 gatgcgacga tagatcacgt cgaccttggt cggaccgcgc gtcgtccgca tgtagacggt
    15301 gtcgtggtcg acgaacagat cgcgcccctc gacgagttca acgcccattt gctgcgcgag
    15361 gaaggcgtgt tcgaagtagg cgctgttgta ggcgccaggc gacatgacga caacggtcgg
    15421 atcgtcgatt gcatcgggcg cgactgcgcg cagcgtgtcg agcaacaggt ccgggtaatg
    15481 gtcgaccggc gcaacccgtt gcatggcaaa cagttcgggg aacagccgca tcatcatgcg
    15541 ccggtcttcg agcatgtacg acacaccgga cgggacacgc aggttgtctt cgaggacgta
    15601 gaactcgccc tcgccggcac gcacgatatc gacgccggcg atctgcgcgt agatgtccgc
    15661 cgcgacgtcg acgccctgca tctccttgcg gtactgcgcg ttgccgagca cctcttccga
    15721 cggaacgatg cccgacttca gtattttctg gtcgtggtag atgtcgtgca ggaacatgtt
    15781 gagcgcgcgc acgcgctgct tgaggccgtt ctgcagcgtc gcccactcgt gtgccggaat
    15841 gatccgcggc acgatgtcaa aggggatcag ccgttccttg ccggactcct cgccgtagac
    15901 ggcaaaggta atccccatgc ggtggaaggt cagatcggcc tcggcccgct tccgcttcaa
    15961 tcgctcggac gccatgttgg ccagccagtt ctcgaatgat tcatagtgcg aacggatcgc
    16021 accggacgaa tccgtcatct catcgaaaaa tttgttcggc attgcacccc ctagcgttga
    16081 tcgtatgacg cacggagcat gccagcgcaa gaccgtgcgc cgacgcccgc ctggccgtat
    16141 cgattgcaga aacctcggga aaacttagca ccaattcggt gcagccaaga ctcaagttgg
    16201 ggctgctgcg ctgcaggatg aaccggcgca acgcagtgac tcggtgctac agaaccgcaa
    16261 cccccttgat ttgcgcgacc acttcgcgac cgggtcgaac gccaagcaaa gccacggatc
    16321 gttcggtaat tcttgagagc aagtatacgc ccttggcatt gacggtcacg agcgcgcgtc
    16381 cgggaccgtc cgggaccacc gcttcgaccc tggccgggat caggttgagg atgctggttt
    16441 cgcgggcctt ttcgatggcg atgctgacgt cacgcgcgag gacgcgcgct cggactgcgg
    16501 tgccgatcgg caatgccagt tgggaaaccc agaccggacc tgccgcacag tcgattcgcg
    16561 tcagccgata gacgtcatcc tggaccgcga cagtaccgtc gatgatcgcc gaagcttcgt
    16621 caccgcgtgc caatggcagg tcggttcgcg ttagcgtcgc cgccgtaggg ccgctggcct
    16681 gtacccggcc gtcggcgagc agcaccagat gatcggccaa tcgagcgacc tcgtcgattg
    16741 catgggtgac gtagagcacg gggatattga gttcgcgatg caggcgttcg aggtagggca
    16801 tgaactcggc cttgcgcgcc gcgtcaagtg ccgccagcgg ttcatccatc agcagcagtc
    16861 gcggcgacgt caccagcgcc cgagcaatgg caacgcggct ccgctcgccg cctgacagcc
    16921 gctcgggctt gcgaccgagc agcggtgata tccccagcaa gtcaacgatg gcgtcgaatc
    16981 cgctttctgc cgcgccagca cggcgcaaac catactcgat gttggcgcgc accgagagat
    17041 gcggaaacag gctggcttcc tgaaagacgt agccgagcgg ccgcgcgtgc gttggcaggc
    17101 agcctgtcgc gtcctgccag acctcgccac cgacgaccag acggccatca tcgacgcgtt
    17161 cgaggccagc gatggcccgc aacaaggtcg tcttgcccga gccagaatga ccaaagaacg
    17221 cggtgatacc gtgacccggc agcgtcaggt cgacgtcgag cgagaagccc gggtaggcga
    17281 gcttgaagca cgcggtgatg ccggcatccg cgccgctggc tgcgaccgca gtcacgaccg
    17341 cttcctagtt ggcgccagtg aataaagcgc cagcaacacg agaaaggaaa agacgatcat
    17401 gccagctgcc agccagtgcg cctgcgcata ctccatcgcc tcgacgtgat cgtagatctg
    17461 taccgagacc acgcgcgtcg cgcctggaat gttgccgccg atcatcagca cgacgccgaa
    17521 ctcaccgacc gtgtgcgcga aaccgaggat tgctgcagtc aggaaccctg gccgcgccag
    17581 gggtaccgct acgctgaaga acgcatcaag cggtcgggcg cgcagcgttg ccgcgacttc
    17641 gagcggtcgc tcgccgatcg cctcgaaggc attctggatc ggctgcacga caaagggcat
    17701 cgagtagaac accgaggcga cgacgaggcc ccagaaggtg aacggcagaa gaccaatgcc
    17761 cgcccactgc gtaaactgcc ctaccgggcc gttcggcccc atcagcagca gcaggtagaa
    17821 gccgatcacc gtcggcggca gcacgatcgg catcgccacc agtgcgccga ccggaccccg
    17881 ccaggccgag cgcgttcgcg ccaaccacca ggcgatcggc gtgccgatga ccagcagcac
    17941 gaccgtggtc acggtcgcca gtttgagcgt cagccagatc gcggcgaggt catcgggact
    18001 cagcatggaa gcgaagctta ccgttaggcg tggtggcgcg acagggctgc cgcggcgcgg
    18061 ttcagtggcg tgagaacagg tcgcgcagtc tgcagagcgc ggccgtcatg tcaaatcgcg
    18121 gatcggccaa ggactcaccg cgcaggatcg tggcaagcgc gacatcggcg tcacgctcgc
    18181 cggtcagcaa cacgctcgcg ccccactgct gcatgtggcg gacaaagccc tcccctgcac
    18241 tggccgcgac gaaaacatcg acgccgtgca atgggtgggg cccgtcatcg cggaaataat
    18301 gcggcaactg cgcccggctt aggcagatcg cctgcggcac cggcggaggc attccgggct
    18361 ggcagtcgaa caccacccag tccggcgtct gtcccgcgtg accggaaacc gtacgcagat
    18421 ccggcttgac ggcaatagcg atcttcatcg tctgggatag gccgttgctc aggggacgac
    18481 ctcgtatcca tgattacgga tgatccgtcg agcaacctcg gtcgacagga aatccatgaa
    18541 agcggctgcc gctcgattgt tctttgcttc cgtaaccatc accgcgtcct gtcgaatcgg
    18601 ggaatgcaat tcagacggca cgacccagcc ggagccagca cccacgcgac catcgacgat
    18661 cacttgcgac agggccacga agccaagctc ggcgttgccg ctggcaacga actgatgggc
    18721 ctgcgcgatg ttctccgcct gaactaggcg cggggtggcg atatccagaa cattcagctt
    18781 gcccaacact tcgcgcgccg ctgcgccgta gggagccaat ttcggattgg cgatcgccag
    18841 cttgcgaaac tgcccctgcc gcaacactgc gccctgcgga tcgacgacgc cggcccgggc
    18901 cgaccacaga acgaggcggc cgatggcata ggtcatccga ctgcccgcca gccccaggcg
    18961 ctcgtcgacc aggcgccgcg gcgtctcaga atcggccgac agcaacacat cgaacggcgc
    19021 gccgttgcgg atctgcgcat agaacttgcc ggtcgagccg gtggcaatcg tcgcccgatg
    19081 cccagtcttc tgctcgaatt cctcgaccag caccttggct ggccgcgcga aattcgaggc
    19141 aacggccaca cgaaccagct cggccgccgc cgagaaaggc agaaggctca acgcgacgga
    19201 aagaaacagg cgcggcagac ggattgcggc cagggaatgg gagcgcatgc gcgcactata
    19261 gcgacacggc caatgaccag ctcatccgct tgatcggtac gtcctcgggt ccaaaacact
    19321 cggcttggtt ggcacagacg ccgcaggatg gcgctcggca ggcgcgcacg tcgagccgtt
    19381 cgcagagttg cttgtagaag aactttttcc agcgcatgcg acccacgttg agcgccgcca
    19441 agttcgggaa atgcgtgtag agaatccgcg acagttcgtc gcgtgtcgcc agcccgaggt
    19501 cgtgccagag gtggtcgtgc ccaagacagg cacgcgccat cgccgccgcc agttcggcca
    19561 cggcgggatc agcagcgttg attgcgccgt ccagcaatag gtcacgcagg tcctcgaact
    19621 cctcgttgcg ctcgccggcc tccgccattt cgcctccggc ccgccaacga tgatcgatca
    19681 gccgcaggtc ggcgccggcg tcggaatcag agcgcgcgcg gtgcatgact gatcgccttc
    19741 ttgccgcaga ccttgccgca ggattcgcag ccgacacagt tctcgggatg cccgaccgtc
    19801 atcaccttct tctcgtactc gtcggcgtca tcgtcgtcgt cgagagcgac gtattcgccg
    19861 tcgtcgtcga cccccatcag ttcgagaacc tctcgcgcgc aggtcttgaa gcagcgaccg
    19921 cagccgatgc acttggctgg atcgacgccg gtggcgaaat gaggcgtcca ttcaacgccg
    19981 gacggcaagg tgacggtaaa ggtgctcatc gttcgctcct gtaggaatcc ttggtgttca
    20041 ggacgacgcc ttggccgggg ccagcacggc ggcttcgctt tttgctgccg caagggccgc
    20101 gaacgcgtcg taggtctttt gcgcctgttc catgattcgc tcccacccca gcggcagctc
    20161 ctcggacaga tcgtgcagat ccatctttgc ctgcgtggcg cgcgcggaaa gcttgcgcat
    20221 gtcgattgat ttctcatcgc ccatcgtgtt ctccttcgat cacatttctg cgagatcgcg
    20281 gtagcgctga atcaggttgc cggcctcggc aacgaccctg tcgccgccct cggccagctt
    20341 ctccagcgaa tcaaaaccga accggtgaac gtcgcggtga tgcttgctgt aagccaccac
    20401 gcggccggcg agcaggactg cccgcccaaa gccttcgtga ttgatcttga tcatcggcgc
    20461 acacatgatt ccggtggccc gctcgatcga caggccgatc gcgttgtaat agagctccag
    20521 ccgccagagc acgtcgggat ccgggtcgtc gatgatcggc atgg
//
LOCUS       DATDAA010000396         4950 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_168966,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000396 DATDAA010000000
VERSION     DATDAA010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168966"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..198)
                     /locus_tag="VIS73_11905"
     CDS             complement(10..198)
                     /locus_tag="VIS73_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763902.1"
                     /translation="MDQTPGRIDADHEPELSDEDILDAMQRVPGYIDISTEDFRVIYH
                     LAWRHAVERLRTRAGDQR"
     gene            345..1208
                     /gene="pstC"
                     /locus_tag="VIS73_11910"
     CDS             345..1208
                     /gene="pstC"
                     /locus_tag="VIS73_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY5763903.1"
                     /translation="MERFVEGMLFLAAMVSVFTTLAIVAILVWESIVFFRHVPIIDFL
                     TDKEWTPLFADAHFGIAPLLAGTLTTTLVALCVAIPIGTIIAIYLSEFASHRLREIAK
                     PFLELLGGIPTVVYGFFALLVVTPVLQKLFPDLPGFNMLSAGLVMGVMIIPYVSSLSE
                     DAMRAVPMQMREGSYAMGATRLQTALFVVTPAAFSGIASSYILGISRAVGETMVVAIA
                     AGMQPNLTMDPTEPASTITAYIVQVALGDLPHGSIGYQTIFAAGLTLMLLTLVFNIIG
                     HILRRRFREAY"
     gene            1208..2149
                     /gene="pstA"
                     /locus_tag="VIS73_11915"
     CDS             1208..2149
                     /gene="pstA"
                     /locus_tag="VIS73_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY5763904.1"
                     /translation="MQQPVHKPTFDVAATRALIAKHKRYDLIFAAIGLIALTAATLVF
                     VALFADMISKGADRLSLEFFTNFPSRRAGQAGILSAWVGSILVMIVTAASAIPIGVAA
                     GLYLEEYGRKNWFTDVIEINVTNLAGVPSIVYGLLALGLFVYAFGFGQSILTAGLTLG
                     LLILPVVIVATREAIRTIPQIIREAAYACGATKWQLVAHHILPYSLPGILTGVIIGLA
                     RAIGETAPVITIGALTFIAFLPESPISSEAPYVNADWLFSPFTVMPIQMFNWISRPEE
                     AFHLNAAAAGLVLMAMTISMNAVAIAIRYHYRKRIKW"
     gene            2143..2925
                     /gene="pstB"
                     /locus_tag="VIS73_11920"
     CDS             2143..2925
                     /gene="pstB"
                     /locus_tag="VIS73_11920"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY5763905.1"
                     /translation="MVTESKAAPIKANAKALNFYYGDFQALKNINFPIEENRITALIG
                     PSGCGKSTFLRCFNRMHDLYPNTRYEGDITMQPDGVNIISNTVDPIEVRMRISMVFQK
                     PNPFPKSIYDNVAYGLKVRGVRNRNYIDQKVEEALRGAALWDEVKDRLNDLAFNLSGG
                     QQQRMCIARALATEPEILLFDEPTSALDPIATAKIEELVTELRKRLTILIVTHNMQQA
                     ARVSDKTAFMYLGEVIELDDTDKIFTNPSSKLTEEYITGRFG"
     gene            3163..3663
                     /locus_tag="VIS73_11925"
     CDS             3163..3663
                     /locus_tag="VIS73_11925"
                     /inference="COORDINATES: protein motif:HMM:NF040826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HEY5763906.1"
                     /translation="MNARSPNNGFRPARRDQLRQERVHDSYKLRGKLPEPAVCSQCGA
                     VYHAGRWQWGKRPTGAQEVACAACQRIADHYPAGFVHVGGDFFAARRDEIIRLLRHHE
                     EKQKAHHPLARIMAIEDEAGGVVLTTTDLHLAHDLGEALYHAYQGDLDFHYNEGETLL
                     RVHWRH"
     gene            3669..>4950
                     /locus_tag="VIS73_11930"
     CDS             3669..>4950
                     /locus_tag="VIS73_11930"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5763907.1"
                     /translation="MEAPTAALDRPPPLAAERLRRRCDPAELGFATTAEVEEPVDIVD
                     QSRAIDAVRFGIAIKRAGFNLFVLGSPGSGRHAAVRRLLLARAADEPVPGDICYVNNF
                     TEPDRPCLLQLPPGRGAKFKADMEQFVAELATAITAAFESDEYRTRIEAIHEEFKEKE
                     EKALRELGQASAETGVALLRTPHGFAFSPIKDEETMKTEDFEALPDEEKKRYAKLMEE
                     HGEQLARLMHQIPRWRRKMEVRIKDIGRETMQFAVGHLIEDLKGHYTDLANVGSFLDA
                     VMDDVIESAEELREAPKTEDGIATITGMLPLGRYQVNLFVDNGTTGAAPVVVEDNPIY
                     PNLVGRVDHVAHMGTLVTNFTMIVAGALHRAQGGYLMLDADKLLRHPYSWEGLKRALK
                     SGQVRIESLGQIYGLTSTESLTPEPMPLSVKVVLV"
BASE COUNT          929 a         1693 c         1432 g          896 t
ORIGIN      
        1 aacggccact caacgctgat ctcccgcgcg agtgcgcagc cgttcgaccg cgtgccgcca
       61 cgccaagtgg taaataaccc gaaaatcctc ggtactgatg tcgatgtagc ctggaacccg
      121 ctgcatcgcg tctaggatgt cctcgtcgct gagttcgggc tcgtggtcgg cgtcgatgcg
      181 ccctggcgtc tggtccatga tttctccgca ggcgattgcc ttggttcttg tcgcacggat
      241 gtaacaattc cttcacataa tggcgcccac tgctaccacg cgccatcccc ctacctgtgc
      301 ttccagatcc caattccttc gcctaccgct tccggcgctt ctacatggag cgtttcgtcg
      361 aaggcatgct gttcctggcc gcgatggtct cggttttcac gacgctggcg atcgtcgcta
      421 tcctcgtctg ggaatcgatc gtcttcttcc gccacgtgcc gatcatcgat ttccttaccg
      481 acaaggaatg gacgcccctc ttcgcggacg cccacttcgg catcgcgccg ctgctcgccg
      541 gcacgctgac cacgaccctc gtcgcgctgt gcgtggcgat cccgatcggc acgatcatcg
      601 caatctatct gtcggaattc gcctcgcacc gcctgcgtga gatcgccaag cccttcctcg
      661 aactgctggg cggcattccg accgtggtct acggcttctt cgcgctgctg gtcgtcacgc
      721 cagtgctgca gaagctgttc cccgacctgc cgggattcaa catgctctcg gccggcctgg
      781 tgatgggcgt gatgatcatt ccctacgtca gctcgctgtc ggaggacgcg atgcgggcgg
      841 taccgatgca aatgcgcgaa ggctcctacg cgatgggggc gacacggttg cagacggcac
      901 tgttcgtcgt taccccggcg gcgttttcgg gaatcgcctc ctcctacatc cttggcatct
      961 cgcgcgccgt cggcgagacc atggtcgtcg ccatcgccgc cggcatgcag ccgaacctga
     1021 cgatggaccc gaccgaaccg gcgtcaacga tcaccgccta catcgtccag gtggccctcg
     1081 gcgacctgcc gcacggctcc atcggctacc agactatctt cgccgcgggc ctgacgctga
     1141 tgctgctgac gctggtcttc aacatcatcg gtcacatcct gcgtcgccgt ttccgcgagg
     1201 cctactgatg caacaaccag tgcacaaacc gaccttcgac gttgccgcca cgcgcgcgct
     1261 gatcgccaag cacaagcgct acgacctgat cttcgcggcg atcggcctga tcgcgctgac
     1321 cgccgcgaca ctggtcttcg tcgcactgtt cgccgacatg atcagcaaag gcgccgatcg
     1381 cctgtcgctc gaattcttca ccaactttcc gtcgcgccgc gccggccagg ccggcatcct
     1441 ttcggcctgg gtcggttcga tcctggtgat gatcgttacc gccgccagcg ccatcccgat
     1501 cggcgtcgca gccggcctct acctcgagga atacggccgc aagaactggt tcaccgacgt
     1561 gatcgagatc aacgtcacca acctcgccgg cgtgccctca atcgtttacg gcctgctcgc
     1621 cctcgggctc ttcgtctacg ccttcggctt cggccaatca atcctcaccg ccggcctgac
     1681 gctgggcctc ctgatcctgc cggtggtgat cgtcgccacc cgcgaggcta tccgcacaat
     1741 cccacagatc atccgcgaag cggcctacgc gtgcggcgcc accaagtggc aactggtcgc
     1801 tcaccacatc ctgccctact cgctgcctgg cattctcacc ggggtgatca tcggcctcgc
     1861 ccgagccatc ggcgagacgg cgccggtgat caccatcggc gcgctgacct tcatcgcctt
     1921 cctgcccgaa tcgccgatca gcagcgaggc gccctacgtc aacgccgact ggctgttctc
     1981 gcccttcacg gtcatgccga tccagatgtt caactggatc tcgcggccgg aagaggcgtt
     2041 tcacctcaat gcagcggcag cgggtctcgt gctgatggca atgacgattt ccatgaacgc
     2101 ggtggcgatc gccatccgct accactaccg gaagagaatc aaatggtgac cgaatccaaa
     2161 gccgccccga tcaaggccaa cgccaaggcg ctgaacttct actacggcga cttccaggcg
     2221 ctgaagaaca tcaacttccc gatcgaggaa aaccgcatca cagccttgat cgggccgtcg
     2281 ggctgcggca agtcgacctt cctgcgctgc ttcaatcgca tgcatgacct ctacccgaat
     2341 acacgttacg agggcgacat caccatgcaa cccgacggcg tcaacatcat cagcaatacg
     2401 gtcgacccga tcgaggtgcg catgcgcatc tcgatggttt tccagaaacc caacccgttt
     2461 cccaagtcga tctacgacaa cgtcgcctac ggcctcaagg tgcgcggcgt gcgcaaccgc
     2521 aactacatcg accagaaggt cgaggaagcg ctgcgcggcg ccgcactgtg ggacgaggtc
     2581 aaggaccgac tcaacgacct cgcattcaac ctgtcaggtg gccagcagca gcgcatgtgt
     2641 atcgcccgcg cccttgccac cgagccggaa atcttgctct tcgacgagcc aacctcggca
     2701 ctcgacccca tcgccaccgc gaagatcgag gagctcgtca ccgaactacg caagcgcctg
     2761 accatcctca tcgtcacgca caacatgcag caggccgcgc gcgtctccga caagaccgcc
     2821 ttcatgtacc tcggcgaagt catcgaactc gacgacaccg acaagatctt caccaaccct
     2881 tcgtcgaagc tgactgagga atacattacg ggtcgattcg gttaatcaac ccgcgatgtc
     2941 cgtgatgtat cggtaaaggc tgccccccgc cacggcggtc tattggcgaa gccgacggcc
     3001 gcgaaaccaa tatccggtgt tctggccgag tccggcccca acttgggttg cattgcggcg
     3061 accaaagtcc gtgcccaata aggccgttct gcgcagcttt ccaatcatcg cgccccggct
     3121 ggactatgct tagtgcagga ttgttcgtga gggagaaaaa tcatgaatgc acgcagtccg
     3181 aacaacggat tccgcccggc acgacgggac caacttcgcc aggagcgcgt tcacgacagc
     3241 tacaagctgc gtggcaagct tcccgagccc gccgtctgca gtcagtgcgg cgccgtctat
     3301 cacgccgggc gctggcagtg gggtaaacgc ccgacgggcg cccaggaagt tgcctgcgcc
     3361 gcctgtcagc ggatcgccga tcactatccg gccggtttcg ttcacgtcgg cggcgacttc
     3421 ttcgccgccc ggcgcgacga gatcattcgc ctactgcggc accacgagga aaagcagaag
     3481 gcacaccatc cgctggcgcg aatcatggcg atcgaggacg aggccggagg tgtcgtgctg
     3541 accaccactg acctgcatct ggcgcacgac ctcggcgaag cgctgtatca cgcctatcag
     3601 ggcgacctcg acttccacta caacgagggc gagaccctgc tgcgcgtgca ctggcggcac
     3661 tagtcatcgt ggaggcgccg accgccgccc tggatcgccc ccctccgctg gccgccgaac
     3721 ggcttcgccg ccgctgcgac ccggcggagc tgggcttcgc cacgaccgca gaggtcgagg
     3781 agccggtcga catagtcgat caatcgcgtg cgatcgacgc ggtgcgtttc ggcatcgcga
     3841 tcaagcgcgc cggcttcaac ctcttcgtgc tcggctcgcc gggcagcggc cgccatgccg
     3901 ccgtacgccg gctcctcctt gcacgggccg ccgacgagcc ggtccccggc gacatctgct
     3961 acgtcaacaa ctttaccgag cctgaccgac cgtgcctgct gcaactgccg ccgggtcgcg
     4021 gcgcgaagtt caaagccgac atggagcagt tcgtcgcaga actggcgacc gcgattaccg
     4081 ctgcctttga gagcgacgaa taccgaacgc gcatcgaggc gatccacgag gagttcaagg
     4141 aaaaggaaga aaaggcgctg cgcgagcttg ggcaggcctc ggccgagacc ggcgtcgcgc
     4201 tgctgcgcac gccgcacggc ttcgccttct caccgatcaa ggacgaggag acgatgaaga
     4261 cggaggactt cgaggcactg ccagacgagg agaagaagcg ctacgcgaag ctgatggagg
     4321 agcatggcga acagctggcg cggcttatgc accagatccc gcgctggcgc cgcaagatgg
     4381 aggtccggat caaggacatc ggccgtgaga cgatgcaatt cgccgtcggt cacctgattg
     4441 aggatctcaa gggccactac accgacctcg ccaacgtcgg tagttttctc gacgcggtga
     4501 tggacgatgt gatcgaaagc gccgaggagc tgcgcgaggc accgaagacc gaggacggca
     4561 tcgcgacgat caccggcatg ctgccgctgg gacgctacca ggtgaacctg ttcgtcgaca
     4621 acggcaccac cggcgcggcg ccggtggtgg tcgaggacaa cccgatctac ccgaacttgg
     4681 tcgggcgcgt tgatcacgtc gcgcacatgg gtacgctggt caccaacttc acgatgatcg
     4741 tcgccggcgc actgcatcgc gcgcaaggcg gctacctaat gctcgacgcc gacaagttgc
     4801 ttcggcaccc ctactcgtgg gagggtctga agcgcgcact gaagtctggt caggttcgga
     4861 tcgagtcgct gggacaaatc tacgggctaa ctagcacgga atcgctcacg cccgaaccga
     4921 tgccgttgtc ggtcaaggtc gtgctggtcg
//
LOCUS       DATDAA010000397         4142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_98753,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000397 DATDAA010000000
VERSION     DATDAA010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98753"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VIS73_11935"
     CDS             <1..697
                     /locus_tag="VIS73_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5763908.1"
                     /translation="LDCGFTIRETEARLARLGLAPDGIAGILLTHEHGDHAAGVFRFA
                     RRHSLPVWLTYGTCSACEANIGDEVDIRLIDSHAPFAVGDIEVRPFPVPHDAREPTQF
                     VFSDGGSRLGVLTDIGESTRHVEQMLSGVDALVIECNHDRAMLAASRYHDSLKRRIGG
                     RLGHLANDLAAGIVAAIDVSRLQHLVAAHLSAENNRPELAVAALAVAAGCEASWIDVA
                     DQEQGLAWRDVNA"
     gene            complement(710..1186)
                     /locus_tag="VIS73_11940"
     CDS             complement(710..1186)
                     /locus_tag="VIS73_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763909.1"
                     /translation="MSRKLLLSWSDLQQARLETLSRADADIAIFDRDLVRFALGSREI
                     DAILRQLLSSKPNARLRIALHDTARATTTQPRLLQLLLRFGHAVTIRETPENLRQLAD
                     EMLIVDSRDAVIHFQFDQPRGKVIGDDPDEIQPYLRRFEELWAECGPPISATSLGL"
     gene            1333..2643
                     /gene="rlmD"
                     /locus_tag="VIS73_11945"
     CDS             1333..2643
                     /gene="rlmD"
                     /locus_tag="VIS73_11945"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEY5763910.1"
                     /translation="MPTAHIESLDYEAQGVARVDGKTVFVEGALSGEVVEYASYRRKP
                     TFERAVATEIRQSSPQRVVPRCPSFGVCGGCSMQHLDFSAQVAAKQRVLEDNLWHIGR
                     LRPDQVFAPIYGQAWGYRHRARLGVRLVPKKGGVLVGFHERRSSYICDMQSCEVLPAS
                     VSKLLVPLRAVVGSLSIARRVPQIEVSIGGAQIVLVFRILEPLEAADHLILRDFAQSA
                     GVNVMLQPKGPDSVVPLWPVEPDPLSYELPEFGISIGFLPTDFTQVNHEINAVLVRRA
                     LALLQPMAGERVADMFCGLGNFSLPIARSGAYVVGVEGSDGLVDRARQNAARNGLASQ
                     TEFHAANLFAVDPESLERWGGFDKMLIDPPREGAAELVRALPGEKGPERIVYVSCNPA
                     TLARDAAVLVTEKGYRLAGAGVVNMFPQTSHVESIALFLKSGGD"
     gene            complement(2690..2848)
                     /locus_tag="VIS73_11950"
     CDS             complement(2690..2848)
                     /locus_tag="VIS73_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763911.1"
                     /translation="MPEQVALTGPYSQKTQSCETNYVSATLTLYLDICSICASLLRLI
                     MALAGERD"
     gene            complement(2841..4034)
                     /locus_tag="VIS73_11955"
     CDS             complement(2841..4034)
                     /locus_tag="VIS73_11955"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5763912.1"
                     /translation="MLLIRDLCAQLLRISTETVAGKDSAIGVEADKLAGRLLTAKGAD
                     DFSRVAADLKRFVFRLTLAGDDSNDLNLAVLHLLQLLVENVGELIVDDQWLHGQIAVV
                     KDLIAAPRNLRAIEDAERRLRDVIIQQSTLKASLLEARDAMKEMLSGFVQRLAHFADD
                     TSHYHDKIEVCARKISAAADIRDLNDVLREVMQETRAIQLTAQRSRDELREARQRVND
                     AEQRIQQLQDELGKASDLVRHDQLTGMLNRRGLEEAFEREISRSARHDTPICLSVLDI
                     DDFKKLNDSLGHQVGDAALVHLTEVIRGAVRPQDTVARYGGEEFVIIMPDTVLDDARE
                     ALVRLQRALTKRFFLHDNQRLLITFSAGVTQLAAGCTRDEAVKRADELMYEAKRAGKN
                     RVIGA"
BASE COUNT          681 a         1259 c         1382 g          820 t
ORIGIN      
        1 gctcgactgc ggctttacca tccgcgaaac tgaggcgcga ctggccaggc tcgggctcgc
       61 accggacggc attgccggca ttctgctgac ccacgagcac ggcgatcacg ccgccggtgt
      121 attccgtttc gcgcgccggc attcgctgcc ggtctggctc acctacggaa cctgttcggc
      181 ctgcgaggcg aatatcggcg acgaggtcga tattcgtctt atcgattcgc acgcgccgtt
      241 cgccgtcggc gatatcgagg ttcggccgtt tccggtgccg cacgacgcgc gcgagcccac
      301 ccagttcgtg ttttcggatg gcggctcgcg gctcggcgta ctgaccgata tcggcgaatc
      361 gacccgccat gtcgagcaga tgctgagcgg cgtcgatgcg ctggtgatcg agtgcaacca
      421 cgaccgcgct atgctcgcgg cctcccgtta tcacgactca ctcaagcggc ggatcggcgg
      481 ccgcctcggc cacttggcca acgatctcgc ggccgggatc gttgctgcga tagacgtgtc
      541 gcgcctgcaa catctggtcg ccgcccacct gagcgcggaa aacaaccgac ccgagcttgc
      601 cgttgcggcg ctggctgtcg cagcgggttg cgaagcgtcg tggattgacg ttgccgatca
      661 ggagcagggc ctagcgtggc gcgacgtcaa cgcctgacga tgtttcgcct cagagcccaa
      721 ggctggtggc gctgatcggc gggccgcatt cagcccagag ctcttcgaaa cgtcgaaggt
      781 agggctggat ctcgtcgggg tcgtcgccga tgaccttgcc gcgcggctgg tcgaactgga
      841 agtgaatcac cgcatcgcgt gaatcgacga tcagcatctc gtcggcgagt tgccgaagat
      901 tttccggcgt ttcccggatc gtgacggcat ggccgaagcg tagcaggagc tgcagcaggc
      961 gcggctgcgt ggtcgtcgcg cgggcggtgt cgtgcagcgc gattcgcagg cgggcgtttg
     1021 gcttggacga gagcagctgg cgcaggatcg cgtcgatctc gcggctgccg agcgcgaaac
     1081 gcacgaggtc gcgatcgaag atggcaatgt cggcgtctgc gcgcgacagg gtttcgagtc
     1141 gcgcttgctg caggtcgctc cagctgagca gcaattttcg gctcatggtt tcggtaaatc
     1201 gtgaggtatt gacggattac ccgatgatta tcgggttcgc gcaaaagctc aagtggggcc
     1261 ggcgagctcg tcgagtcgtc gcggctcagc caagcgccgg tagaatcccc ccctttttcc
     1321 gggattgagc cgatgccaac tgcccatatc gaatcgctgg actacgaggc gcaaggcgtt
     1381 gcacgggtcg atggcaagac ggttttcgtc gaaggggctc tttccggcga agtcgtcgag
     1441 tacgcgagct atcgccgcaa gccgaccttc gaacgcgcgg tcgccaccga gatccgacaa
     1501 agctcaccgc agcgcgtggt gccgcgctgt ccgagttttg gggtgtgcgg cggctgcagc
     1561 atgcagcatc tcgatttttc ggcgcaggtg gcagcgaagc agcgggtcct cgaggacaac
     1621 ctctggcaca tcggtcgtct tcgtccggat caggtcttcg cgccgatcta tggccaggca
     1681 tggggctacc gccatcgggc ccggctcggc gttcgattgg tgccgaagaa gggcggggtt
     1741 ctcgtcgggt ttcatgaacg gcgcagcagc tatatctgcg acatgcagag ctgcgaagtc
     1801 ctaccggcaa gcgtgtcgaa gctgctagtg ccgctgcgtg cggtcgtcgg atcgctgagc
     1861 attgcccgcc gtgtgccaca gatcgaagtg tcgatcggcg gtgcgcagat cgtgctggtc
     1921 ttccgcatac ttgagccgct ggaagccgcc gatcacctaa ttcttcgcga cttcgcgcag
     1981 tcggccggcg tcaacgtgat gctgcagccg aaggggccgg attcggtcgt cccgctgtgg
     2041 cccgtagagc cggatccgct gagctacgag ctgcccgagt tcggaatcag catcgggttc
     2101 ctgccgaccg acttcacgca ggtgaatcac gagatcaacg cggttctggt ccgtcgtgcc
     2161 ttagccttgc tgcaaccgat ggcgggcgag cgggtcgccg acatgttctg cgggctcgga
     2221 aacttctcgc tgccgatcgc gcgttcaggt gcctacgtgg tcggcgtcga aggcagcgat
     2281 ggactggttg accgcgcgcg gcagaacgcc gcgcgaaacg gcttggcgtc gcagaccgag
     2341 ttccacgcgg cgaacctgtt cgccgttgat ccagaatcgc tggaacgctg gggcggcttt
     2401 gacaagatgc tgatcgatcc gccgcgcgaa ggcgctgcgg agcttgtcag ggcactgccg
     2461 ggagagaagg ggccggagcg aatcgtctac gtgtcctgca acccggcgac gctggcgcgc
     2521 gacgccgcgg tcctcgtcac cgaaaagggc taccgattgg ccggtgccgg ggttgtcaac
     2581 atgtttccgc agacctcgca cgtcgagtcg atcgcgctgt tcttgaagtc gggcggcgat
     2641 tgaggaacgg cggctgtggc tgccgtccat cggtttttga ggctagggct cagtcgcgtt
     2701 caccggcaag agccatgatc aggcgcagca ggctggcgca gatgctgcaa atgtcgaggt
     2761 agagcgtcag cgttgccgac acgtagttgg tctcgcagct ctgggttttc tggctgtacg
     2821 gtccggttag ggcaacctgc tcaggcaccg atgacccggt tctttccggc ccgctttgct
     2881 tcgtacatca gttcgtcggc acgcttgact gcctcgtcgc gggtgcagcc agccgccagt
     2941 tgcgtcacac cggcactaaa ggtgatcagc aaccgctggt tgtcgtgcag gaaaaagcgc
     3001 ttggtcagcg cgcgttgcag ccgcaccagg gcctcgcggg catcgtccag aaccgtgtcg
     3061 ggcatgatga tcacgaattc ttcgccgccg tagcgcgcca cggtgtcctg cgggcgaacg
     3121 gcgccgcgga taacctcggt caggtgcacc agcgccgcgt cgcccacctg gtggcccagc
     3181 gagtcgttga gtttcttgaa gtcgtcgatg tcgagtaccg agaggcagat tggcgtgtcg
     3241 tgccgtgccg agcgcgagat ctcgcgctcg aaggcctcct caaggccgcg gcggttcagc
     3301 atgcctgtca gttggtcgtg gcgtaccagg tcgctggcct tgccgagttc gtcttgcaac
     3361 tgctggatcc gctgctcggc atcgttgacc cgctgacgtg cttcgcgcag ttcgtcgcgc
     3421 gagcgttggg cggtcagctg aatcgcccgg gtttcctgca tgacctcgcg caggacatcg
     3481 ttaaggtccc ggatatctgc tgcggcggat atctttcgcg cgcagacttc gatcttgtcg
     3541 tggtaatgac tggtgtcatc ggcgaaatgc gccaggcgct ggacgaagcc cgaaagcatt
     3601 tccttcatcg catcacgggc ttcgagcagg ctggccttca gcgtactctg ctgaatgatg
     3661 acgtcgcgaa gtcgccgctc ggcgtcctcg atcgcccgga ggttgcgcgg cgcggcgata
     3721 agatccttga ccactgcaat ctggccgtgg agccactggt catcaacgat caattcgcca
     3781 acgttttcga ccagcaattg cagcagatgc agcaccgcga ggttgaggtc gttcgaatca
     3841 tcaccggcga gcgttaggcg aaagacgaac cgttttaggt cagctgcaac ccgggagaaa
     3901 tcgtcggcgc ccttggccgt gagaagccgg cccgccaact tgtccgcctc gacgccgatc
     3961 gcggagtctt tgcccgcaac cgtttccgtc gagatgcgga gcagttgggc gcaaagatcc
     4021 ctgatcagga gcatcccatc agtcgagtcc gaagccgctc cggcgcgtac cgacggcggg
     4081 ctttcgtcgg gagcattagc gcttcgagtc ggcgccgcgg cccacttttt tagcagttcg
     4141 cg
//
LOCUS       DATDAA010000398        11201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_63499,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000398 DATDAA010000000
VERSION     DATDAA010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11201
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63499"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VIS73_11960"
     CDS             <1..455
                     /locus_tag="VIS73_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY5763913.1"
                     /translation="IKTVNALSHYTDWTVGHVHSGALGWVAMVSIGSIYYLLPKLFGK
                     SEMYSVKLITAHFWIATIGVVLYIASMWISGVMQGLMWRAVNADGTLTYSFVEAVKAS
                     YDFWAIRFLGGVLFLGGMVLMAYNMFKTIAGGRTDDEPVAQVVAAHHA"
     gene            471..1097
                     /gene="ccoO"
                     /locus_tag="VIS73_11965"
     CDS             471..1097
                     /gene="ccoO"
                     /locus_tag="VIS73_11965"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEY5763914.1"
                     /translation="MKLTHDLIERKLGLMIVLTLLVVSVGGLLEIVPLFFQKSTTTPV
                     EGVKPYTAVQLAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGEFVYDHPFQWG
                     SKRTGPDLHRVGERYSDEWHIAHLMQPRDVVPESNMPAYPWLAERKAKDTVAANIDTK
                     MAALNKVGLHKYSADEIAGAKAAVGDLTEMQVLVAYLQGLGLALKNTR"
     gene            1107..1277
                     /locus_tag="VIS73_11970"
     CDS             1107..1277
                     /locus_tag="VIS73_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEY5763915.1"
                     /translation="MDINDARSLITVLAFATFIGIVIWAYGRGRKAAFDEAAALPFTE
                     EDRPAGAQHPRG"
     gene            1292..2200
                     /gene="ccoP"
                     /locus_tag="VIS73_11975"
     CDS             1292..2200
                     /gene="ccoP"
                     /locus_tag="VIS73_11975"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEY5763916.1"
                     /translation="MSDFVSEFWNIYVVVIVLGSVLACAWLLWAQSKHKPTVSASGDV
                     GTSGHVWDDIEEYNHPLPKWWMWMFYITVVFSLVYLALYPGLGSFGGMLGWTSVGQYQ
                     AERERVDAQVAPIYERFVQMDLKAVAADKEAQEMGRRLYLTYCSQCHGSTAAGAKGFP
                     NLTDKDWQWGGEPEQIVATISQGRVGMMPPFAHLGEAAIVDLANYVRSLAGYQHDAAA
                     AARGAESFVGSGCVGCHGMDAKGNSLMGAPNLTDKTWLYGSSEAEIVSGITNGRQNKM
                     PAWQQFLGDAKVHVLSAYVYSLSNKP"
     gene            2339..3748
                     /gene="ccoG"
                     /locus_tag="VIS73_11980"
     CDS             2339..3748
                     /gene="ccoG"
                     /locus_tag="VIS73_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEY5763917.1"
                     /translation="MSGPNTQKQPLPDSGGMYEKYRKVYAREIKGAFTNWRWIMAWLT
                     QLVFYGLCWIEWNGRQAVLFHLVERKFYLFGLVLWPQDVFYLAVLLIISAYGLFLFTA
                     VAGRLFCGYACPQTVYTLIFMWIENRIEGSRNARMKRDAAQTSGDKALRKGAKYLAWT
                     VLSLWTGFTFVGYFTPIHELWDSVLAWTLGPWETFWIFFYAAFAYLMAGIVREQMCKY
                     ICPYARFQAVMFDPDTLIISYDNARGEPRGARRKAIDYKAQGLGDCIDCGICVQVCPT
                     GIDIRNGLQLECIACAACVDACDQVMDQMNYPRGLVRYSTENALARHYTRKDIYAHLL
                     RPRTLIYSSILLAITAAMIWSLYARIPFKADVIRDRGSLGREIDDGRIENVYTLRLMN
                     TGEVERRYSIAVSGLPSATLDGETEIATPAAATLAVPIAVRAAPDDGKAGANQIFFEI
                     VAMDDPSIRLREKATFMRP"
     gene            3754..4260
                     /locus_tag="VIS73_11985"
     CDS             3754..4260
                     /locus_tag="VIS73_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEY5763918.1"
                     /translation="MDRQLDQQPWYRQRWPWLLMVPPAVAIVAGAVTIWIAVVTSDGL
                     VEDDYYKQGLGVNQRLARDEAAQRLGIGADLMLSADGRSLRAMLSADAPLDAEIRLRL
                     THPTRKGYDQTVSLLRQEDGLYLASLEQAVNGRRIVTLESPGGEWRLVGDWDTSQVDA
                     VALGAPRR"
     gene            4350..4931
                     /locus_tag="VIS73_11990"
     CDS             4350..4931
                     /locus_tag="VIS73_11990"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEY5763919.1"
                     /translation="MQIVIRAMAAVLALATISGNALAGDATTAVGTGIGAAAGAAIGD
                     QQGGKQGAVIGGAVGGAVGAAVSTKGSGKKGAVVGGAIGGASGAAIGHDVGGAQGAVA
                     GAAIGGAAGGVIGKNVTQKEPVNRTAVGVAGGTAATGVVVGTRRGERDDERYRVRDRD
                     DHGKHKHKGKKRKHCDDDHPGRGHAYGKYKNCD"
     gene            complement(5013..5279)
                     /gene="infA"
                     /locus_tag="VIS73_11995"
     CDS             complement(5013..5279)
                     /gene="infA"
                     /locus_tag="VIS73_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY5763920.1"
                     /translation="MAKEEQIEMQGVVEEVLPDSRYRVTLENGHKLIAYTAGKMRKHR
                     IRILAGDPVTLEMSPYDLNKGRITFRHLETRGGGGAPPPRRNRR"
     gene            complement(5615..6826)
                     /locus_tag="VIS73_12000"
     CDS             complement(5615..6826)
                     /locus_tag="VIS73_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5763921.1"
                     /translation="MRADDFLRLALRALLAQRMRSFLTLLGIAVGIAAVILLTSIGEG
                     LHRYVMNEFTQFGTNIVEIKPGKNTGSGGPGGLQTTVRPLTIADARALERIANVTAVT
                     PVIWSNAEVEANVSGNARVRRTVIYGVGPSFLEAFQSSVRAGRFLPNDDSESARAFVV
                     LGAKLKAELFGPENPLGERLRIGGLQFRVIGVMEPKGQILGQDLDDTAFIPTARALEM
                     LNKEGVAQINVSYAEGVPAASVVRAVKAMLIARHGREDFSIVTQEDMLRTLSKILDVL
                     TVAVGALGGISLLVGGVGIVTIMTISVAERTNEIGLLKAVGAPQQTILLLFLGEAVAL
                     SLVGGSLGLAAGAGLAQLLNLFLPALPVHTPWRFVLWAELASALIGLAAGVLPAKRAA
                     GLDPVEALHAE"
     gene            complement(6831..8030)
                     /locus_tag="VIS73_12005"
     CDS             complement(6831..8030)
                     /locus_tag="VIS73_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5763922.1"
                     /translation="MRPADLLHFAARAATGTPLRTLLMVFAMSIGVAAVVVLTALGDG
                     ARRYVMAEFSALGTNLVIVLPGRSETGGFNPANVITNTPRDLTVADAESLLRLPSVER
                     VAPLTVGNSEAAFSGRLREVMIIGTTASYFRLRQLPLAQGSYLPEDSRVSAAVIGAAL
                     RTELFGNEGAIGRMLRVGDRRYRVVGVLAASGQGMGFNVDELVFVPVDSALAMFNSSS
                     LFRIIVEVRSREAIPATRDRVEKALAARHDGELDFTVVTQDAVLATFDKILGALTIAV
                     GGIAAISLAVAGILVMNVMLVSVTQRTGEIGLLKAVGASGRTIRIAFLTEAALLSTAG
                     ALVGFVLGQLGSWTLRLAYPALPAYPPDWAVVAALSTAIVTGLVFGVLPARRAASLDA
                     INALAKR"
     gene            complement(8056..8778)
                     /locus_tag="VIS73_12010"
     CDS             complement(8056..8778)
                     /locus_tag="VIS73_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5763923.1"
                     /translation="MVELAKINRSFTLGDSVVHALREVELEIAAGEYMSIMGPSGSGK
                     STLLNLIGLLDRPNSGSYRLEGRDVTTLSGDEQARVRRDRIGFVFQSFHLVPRLTAAE
                     NIGLPMMLAGIDPAERRKRIDRALADFGLQSRADHRPDQLSGGQRQRVAIARATIMQP
                     AILLADEPTGNLDRATGEEVVRLLESLNESGVTLLVVTHDGGLGERARRRIVMEDGAV
                     QSDTRRQDETPRARTPPAEGQT"
     gene            complement(8863..10008)
                     /locus_tag="VIS73_12015"
     CDS             complement(8863..10008)
                     /locus_tag="VIS73_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5763924.1"
                     /translation="MKPIVKRLLLGIVIIAALVAAIAFLTRPEPIKVVVAAADRGRVE
                     STLSNTRAGTLESCQRTRLSTILGGRIDALLVKEGDRVAQGQVLMRLWNEDQQAQATL
                     AEAQLMVTRQRIDEICTVADNAGREAERQAQLQREGFVSAGREDQARTEAKARRAACE
                     TARADVGQAEARLRAVQVEQRRTVLRAPFAGTVAKIVGEVGEYSTPSPPGVQTPPAID
                     LFDPGCLYVKAPMDEVDAPKIRAGLPARITLDALGKRVFEARVGRVAPVVTALEKQAR
                     TVDVDVLFAKPEEATGLLVGYSADAEIILDTRDSALRVPTAALQEDGRVLLLEGDKLV
                     ARMLKTGVSNWEYTEVLDGLAEGAQVVTSLEREGVRDGASAVVDDRR"
     gene            complement(11012..>11201)
                     /locus_tag="VIS73_12020"
     CDS             complement(11012..>11201)
                     /locus_tag="VIS73_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763925.1"
                     /translation="RGRRRRIRGRHFLFTPGEGARRGLLDTETPGIAGIGSGTRTALG
                     AWRFVRVGRRGFRIARPG"
BASE COUNT         1863 a         3629 c         3860 g         1849 t
ORIGIN      
        1 cgatcaagac cgtcaacgcg ctgtcgcact acacggactg gaccgtcggc cacgtgcatt
       61 cgggcgcgct cggctgggtg gcgatggtgt cgatcggctc gatctactat ctgctgccga
      121 agctcttcgg caagagcgag atgtacagcg tcaagctgat caccgcgcac ttctggatcg
      181 ccaccatcgg cgtcgtcctc tacatcgcct cgatgtggat ctcgggcgtc atgcagggcc
      241 tgatgtggcg cgcggtcaac gctgacggca cgctgacgta cagctttgtc gaggcggtca
      301 aggccagcta cgacttctgg gcgatccgtt tcctcggcgg cgtgctgttc ctcggcggca
      361 tggtgttgat ggcctacaac atgttcaaga cgattgccgg cgggcgcacc gacgacgaac
      421 cggtggccca ggtcgttgcg gcgcaccacg cctgatcagg gagagacgaa atgaaactca
      481 cgcatgactt gatcgagcgc aagctcggcc tgatgatcgt gctcaccctg ctcgtggtga
      541 gcgtcggcgg cctgctggag atcgtgccgc tgttcttcca gaagtcgacg acgacaccgg
      601 ttgagggcgt caagccctat accgcggtgc agcttgccgg tcgtgacatc tacatccgtg
      661 aaggctgcta caactgccat tcgcagatga tccgcccctt ccgcgccgag accgagcgct
      721 acggccatta ctcggtggcc ggcgagttcg tctacgacca tccgttccag tggggcagca
      781 agcgcaccgg tcccgatctc catcgcgtcg gcgagcgcta ctccgacgaa tggcacatcg
      841 cccacctgat gcaaccgcgc gacgtggttc ccgagtcgaa catgccggcc tacccctggc
      901 tggccgaacg caaggcgaag gacacggtcg cggcgaacat cgataccaag atggcggcgc
      961 tcaacaaggt cggcctgcac aagtattcgg ccgacgagat tgccggcgcc aaggcagcgg
     1021 ttggcgacct gaccgaaatg caggtgctcg tcgcctacct gcagggtctc ggtctggccc
     1081 tcaagaacac gcggtgaggt gaagccatgg acatcaatga cgcacgttct ctgattaccg
     1141 ttctggcttt cgcgaccttc atcggcattg tcatttgggc ctacggccgt ggccgcaagg
     1201 ccgcattcga cgaagccgct gcgctgccgt ttaccgaaga agacagaccg gcgggtgcgc
     1261 agcacccgcg cggctgacag ggagatcgaa catgagcgat ttcgttagcg aattctggaa
     1321 catctacgtc gttgtcatcg tgctggggag cgtgctggcc tgtgcttggc tgctttgggc
     1381 gcagagcaag cacaagccga ccgtcagcgc ctccggcgat gtcggtacca gcggccacgt
     1441 ctgggacgac atcgaggagt acaaccaccc gttgcccaag tggtggatgt ggatgttcta
     1501 catcacggtt gtcttcagct tggtctacct cgcgctctat ccgggcctcg gcagcttcgg
     1561 cggcatgctc ggctggactt cggtcggcca gtaccaggcg gaacgtgaac gcgtcgacgc
     1621 ccaggtggcg ccgatctacg agcggttcgt ccagatggac ctgaaggccg tggcggccga
     1681 caaggaagcg caggaaatgg gccgccgcct gtacctgacc tactgttcgc agtgtcacgg
     1741 ctcgaccgcg gccggcgcca agggattccc taatctgacc gacaaggact ggcagtgggg
     1801 gggcgagccc gagcagatcg tcgccaccat ttcgcagggg cgcgtgggca tgatgccgcc
     1861 gtttgcccat ctgggtgagg ctgccatcgt tgacttggcc aactacgtgc gttcgctggc
     1921 gggctaccag cacgatgctg ccgcagccgc tcgcggtgcg gaatccttcg ttggctccgg
     1981 ctgcgtcggc tgtcacggta tggacgcgaa aggcaattct ctgatgggag cgccaaacct
     2041 gaccgacaag acttggctct acggcagcag cgaagctgag atcgtgtccg gcatcaccaa
     2101 cggtcggcag aacaagatgc cagcctggca gcagttcctc ggcgatgcca aggttcatgt
     2161 gctgtcggcc tacgtctaca gcttgagcaa caagccctga acgttttccg ggagcggcta
     2221 ggtccgctcc cggcgtttcg cgttggtcgt acgacgggcc ttgagaattt cgtggccaat
     2281 tttttcacgc cgaaaattag catcccatga aattctgaat gttcttggaa gtgctttgat
     2341 gagcggtccc aacacgcaga agcagccctt gcccgacagc ggcggcatgt acgagaagta
     2401 ccggaaggtc tacgcccgcg agatcaaggg ggcattcacc aactggcgct ggataatggc
     2461 ttggctgacc cagctggtct tctacggcct gtgctggatc gagtggaacg ggcggcaggc
     2521 agtgctcttc caccttgtcg aacgcaagtt ctacctgttc gggctggtgc tttggccgca
     2581 ggatgtcttc tatctggcgg tgctgctgat catctcggcc tacggcctgt tcctgtttac
     2641 ggccgtcgcc gggcgactgt tctgcggcta cgcctgtccg cagaccgtct acacgctgat
     2701 cttcatgtgg atcgagaacc gcatcgaagg cagccgcaac gcccgcatga agcgcgatgc
     2761 ggcccagacc agcggtgaca aggccttacg caagggagcg aagtatcttg cgtggaccgt
     2821 gctgtcgctg tggaccggat tcacctttgt cggctatttc acgccaatcc acgaactctg
     2881 ggattcggtg cttgcctgga cgctcggccc ctgggagacc ttctggattt tcttctacgc
     2941 cgccttcgcc tacctgatgg ccggcatcgt gcgcgagcag atgtgcaaat acatctgccc
     3001 ctacgcgcgc ttccaggcgg tgatgttcga tcccgacacg ctgatcattt cctacgacaa
     3061 cgcgcgcggc gagccgcgcg gtgcccggcg gaaggccatc gactacaagg cgcagggact
     3121 cggtgactgc atcgactgcg gcatctgcgt gcaggtctgc cccactggaa ttgatatccg
     3181 caacggcctg cagctcgagt gcattgcctg cgccgcctgt gtcgatgcct gcgatcaggt
     3241 gatggaccag atgaactatc cgcgcggcct cgttcgttac tccaccgaga acgcgctggc
     3301 tcgccattac acgcgcaagg acatctacgc ccacctgctg cggccgcgga cgctgatcta
     3361 cagctcgatc ctgctggcga tcaccgcggc gatgatctgg tccctttacg cgcgcattcc
     3421 gttcaaggcc gacgtgatcc gtgaccgcgg cagcctaggc cgcgagatcg acgacggacg
     3481 gatcgaaaac gtctatacgc tgcgcctgat gaataccggc gaagtcgaac gacgctacag
     3541 catcgctgtc agcggcctgc cgtcggcgac attggacggc gaaaccgaga tcgcaactcc
     3601 agcggcggca acgctggccg tgccgatcgc cgttcgcgcc gcgcccgacg acggcaaggc
     3661 tggcgccaat cagatcttct tcgaaattgt cgcgatggac gacccatcga ttcgcctgcg
     3721 cgaaaaagcc actttcatgc gaccctgagc atcatggaca gacaactcga tcaacaaccc
     3781 tggtatcggc aacgctggcc ctggcttctc atggtgccgc cggcggtggc gatcgtcgcc
     3841 ggcgcggtga cgatctggat cgccgtcgtc acctccgacg ggctggtgga ggacgactac
     3901 tacaagcagg gtctgggcgt taaccagcgg ctcgcccgtg acgaagcggc gcagcgactg
     3961 ggcatcggtg ccgacctcat gctctctgct gatggccgtt cgctacgcgc aatgctcagc
     4021 gccgacgcac cgctagacgc cgagattagg ctcaggctga cgcatccgac gcgcaagggc
     4081 tatgaccaga cagtaagcct cttgcggcag gaagacggcc tttacctcgc cagtctcgag
     4141 caggccgtga acggtcgacg catcgtcacg ctcgaatcgc cgggcggcga atggcggctc
     4201 gtcggcgact gggataccag ccaggtcgat gcggtcgctt tgggcgcgcc acgacgctga
     4261 cagcggcgct gcgcgccgag aagactccgt ctagaatgcg cacagcggct cgctgattgg
     4321 cagttctgcc gcaacgaagg agtaagtgaa tgcagattgt gatcagggca atggccgctg
     4381 tgttggcgct cgccaccatc agtggcaatg cgctcgccgg cgatgcgacg accgccgttg
     4441 gcaccggcat cggtgctgcc gcgggtgcgg cgatcggcga ccagcagggc ggcaagcagg
     4501 gcgctgtgat tggcggcgcg gtgggtggtg cggtgggcgc ggccgtgtca accaagggtt
     4561 cgggaaagaa aggtgcggtg gtcggtggtg cgatcggcgg cgcttccggg gccgcgatcg
     4621 gccacgatgt cggcggcgcg cagggcgcgg ttgccggcgc cgccatcggt ggcgcggcgg
     4681 gtggcgtgat cggcaagaac gtcacgcaga aggagccggt taaccggacc gcggttggtg
     4741 tcgccggcgg cacggcggcg accggcgtcg tggtcggcac gcggcgtggc gagcgcgatg
     4801 acgagcgcta ccgggtacgt gatcgggacg atcacggtaa gcacaagcac aagggaaaga
     4861 agcgcaagca ttgcgacgac gaccatccgg gtcgtggcca cgcttacggc aaatacaaga
     4921 attgcgactg acaggactgg cggcagcggt cggcagcccg tgcgcgtccg gcgcctgtcg
     4981 tcaggcggcg cgcggaatgg agatggatct tctcagcggc ggttgcgacg cggcgggggg
     5041 gcgccaccgc caccacgggt ctcaaggtgg cggaaggtga tccggccctt gttcaggtcg
     5101 tagggcgaca tctccagcgt caccgggtcg ccggcgagaa tgcggatgcg gtgcttgcgc
     5161 atcttgcccg cggtgtaggc gatgagcttg tgcccgtttt cgagcgtgac gcggtagcgc
     5221 gaatcgggca gcacttcctc gacaacgccc tgcatttcga tctgttcttc cttggccatt
     5281 ctgttctcct gttcggtggc cttcgcctgc ctggccggca ttggcgaacg gcttttttcc
     5341 agtcgcgcag cggtgcaaag cgccgcctgg ataagcggag gttgccttgc ggcgctgaag
     5401 gacccggcat ggccggatcg tgaatcgggg tgaggcgagc cgttcgcgtt ggcggctggc
     5461 tgccggtggt gccgtggccg acgatgaaac cgtcgtcaaa ggcgccgatg gcacccgaca
     5521 ctatgcctga tattgcaccg cggtacaaat ccgcgccttg cgccaacggg cgccgaacgg
     5581 gcgccgaacg gcggcgaacg accgacccga ggtctcattc ggcgtgcagg gcctcgaccg
     5641 gatcgaggcc cgctgcgcgc ttcgccggca gcacgccggc ggccagcccg atcagggccg
     5701 acgccagctc ggcccagagc acgaagcgcc agggcgtgtg cacgggcagc gccggcagaa
     5761 acagattgag cagttgcgcg agtccggcgc cggccgccag gccgagactg ccgccgacca
     5821 gcgacagggc caccgcctca ccaagaaaga gcaggaggat cgtctgctgc ggcgcgccga
     5881 cggccttcag caaaccgatc tcgttggtcc gttcggccac cgagatggtc atgatggtga
     5941 cgatgccgac cccgccaacc aagagcgaga tgccgcccag tgcgccgacg gcgacggtca
     6001 gcacatcgag gatcttcgac agcgtgcgca gcatgtcttc ctgggtgacg atcgagaagt
     6061 cctcgcggcc atggcgggcg atcagcatgg ccttgaccgc gcgcacgacg ctggccgccg
     6121 gtacgccctc ggcgtaggac acgttgatct gcgccacgcc ttccttgttg agcatctcga
     6181 gcgcgcgggc ggtggggatg aaggcggtgt cgtcgaggtc ctggccgagg atctggccct
     6241 ttggttccat gaccccgatg acgcggaact gcaggccgcc gatgcgcagg cgttcgccga
     6301 gcgggttctc ggggccgaac agctcggcct tgagcttggc gccgaggacg acgaaggcac
     6361 gcgccgactc gctgtcgtcg ttcggcagga aacgtccggc gcgtacgctc gactggaagg
     6421 cttcgaggaa gctcggtcca acgccgtaga tcaccgtgcg gcggacccgc gcgttgccgg
     6481 agacgttggc ctcgacctcg gcgttgctcc atatcaccgg tgttaccgcc gtgacgttgg
     6541 cgatccgctc gagcgcgcgg gcgtcggcga tggtcagcgg gcgcaccgtc gtctgcaggc
     6601 cgcccggccc gccgctgccg gtgttcttgc ccggcttgat ctcgacgatg ttggttccga
     6661 actgggtgaa ctcgttcatc acgtagcgat gcagaccctc gccgatgctt gtgagcagga
     6721 tcaccgcggc gatgccgacg gcgatgccga gcagcgtcag aaagctgcgc atgcgttgcg
     6781 cgagcagcgc gcgcaacgcc aggcggagga agtcgtcggc gcgcatcggc tcagcgcttc
     6841 gccaacgcgt tgatggcgtc gagcgacgcg gcgcggcgcg ccggcaatac gccgaacacc
     6901 agcccggtca cgatggcggt ggagagcgcc gctaccacgg cccagtcagg cgggtatgcc
     6961 ggcagcgccg gataggcgag gcgcagtgtc catgagccga gctggccgag cacaaagccg
     7021 accagcgcgc cggctgtcga cagcagcgcc gcctcggtga ggaaggcgat gcggatcgtc
     7081 cgccccgagg cgccgactgc cttgagcagt ccgatttcgc cggtgcgctg ggtaaccgat
     7141 accagcatca cgttcatcac cagaattccg gcgacggcga ggctgatcgc ggcgatgcca
     7201 ccgacggcga tggtcagcgc gccgaggatc ttgtcgaagg tggcgagcac ggcatcctgg
     7261 gtgaccacgg tgaagtcgag ttcgccatcg tggcgtgccg cgagcgcctt ctcgacgcgg
     7321 tcgcgggttg ccggaatcgc ctcgcggctc ctgacctcga cgatgatgcg aaacagcgag
     7381 ctgctgttaa acatcgccag cgccgaatcg accggcacga acaccagctc gtcgacgttg
     7441 aaacccatcc cctggccgct cgccgcgagc acgccgacga cgcggtagcg ccggtctccg
     7501 acgcgcagca tgcggccaat cgcgccttcg ttgccgaaca gctcggtacg aagcgcggcg
     7561 ccgatgacgg cggcggatac gcgcgaatcc tcgggcaggt agctgccctg cgccaacggc
     7621 aactggcgca ggcgaaagta gctggccgtg gtgccgatga tcatcacctc gcgcaggcgt
     7681 ccgctgaagg ccgcttccga attgccgacc gtcagcggtg cgacgcgctc gaccgaaggc
     7741 aggcgcagca gcgactcggc gtcggcaacc gtaagatcgc gcggcgtatt ggtgatgacg
     7801 ttggcaggat tgaagccgcc agtctccgag cgcccgggaa ggacgatcac caggttggta
     7861 ccgagcgccg agaactccgc catgacgtaa cggcgggcac cgtcgccgag ggccgtgagc
     7921 acgaccacag cggcgacgcc gatgctcatc gcaaagacca tcagcagcgt acgcagcggg
     7981 gtgccggtgg cggcgcgggc ggcgaagtgg agcaggtcgg cggggcgcat ggccgtcagc
     8041 gggcgcgtcg tcgcatcagg tttgaccctc ggcgggcggc gtgcgtgcgc ggggcgtttc
     8101 atcctggcgg cgggtatccg actgcaccgc gccatcctcc atgacgatgc gccgacgggc
     8161 gcgctcgccg aggccaccgt cgtgggtgac caccaacagc gtcacgccgg attcgttaag
     8221 cgattcgagc aggcgcacga cctcctcgcc ggtggcgcgg tcgaggtttc cggtcggctc
     8281 gtcggcgagc aggatggcgg gttgcatgat cgtcgcccgg gcgatcgcaa cgcgctgccg
     8341 ctggcccccc gagagctggt cggggcgatg gtcggcacgg ctctgtagcc cgaagtcggc
     8401 gagggcacga tcgatgcgct tcctgcgttc ggccggatca atgccggcga gcatcatcgg
     8461 caggccgatg ttctcggcgg cggtcagacg cggcaccagg tgaaagctct ggaagacgaa
     8521 gccgatgcgg tcgcgtcgca cgcgcgcctg ttcgtcgccc gaaagcgtcg ttacgtcgcg
     8581 gccctcgagc cggtagctgc cgctgttggg ccggtcgagc aggccgatca ggttgagcag
     8641 tgtcgacttg ccagagcccg aggggcccat gatcgacatg tactcgccgg cggcgatctc
     8701 gagttcaacc tcgcgcagtg cgtggacgac gctgtcgccg agcgtgaagc tgcggttgat
     8761 cttcgccagt tcgacgattg tgccgctgtc gctgccgccg gtcggcgacg ccgcggcggc
     8821 gggctggtcc ggatcgggcg gcaagcggcc ggccacggcg cttcagcggc ggtcgtcgac
     8881 aaccgcgctg gcgccgtcgc gaacgccttc gcgctcgagc gaggtgacca cctgcgcgcc
     8941 ttcggcgagt ccgtcgagga cctcggtgta ttcccagttg ctgacgccgg ttttcagcat
     9001 ccgcgcgacc agcttgtcgc cttcgagcag cagcacgcgg ccatcttcct gaagcgcggc
     9061 agtgggcacg cgcagcgccg aatcacgggt gtcgagaatg atctcggcgt cggcgctgta
     9121 accgacgagc aggccggtcg cctcttccgg cttggcaaac aacacgtcga catcgaccgt
     9181 gcgcgcctgc ttctcgagtg ccgtgaccac tggcgcgacg cgcccgacgc gggcttcgaa
     9241 gacccgcttg ccgagcgcgt cgagcgtaat ccgcgcgggt agaccggcgc ggatcttcgg
     9301 cgcatcgact tcgtccatcg gcgccttgac atagaggcag cccggatcga ataggtcgat
     9361 cgccggcggc gtctgtacgc cgggcggcga gggcgtcgaa tattcgccga cttcgccaac
     9421 gatcttcgcc accgtgccgg caaagggtgc gcgcaacacg gttcgtcgct gttcgacctg
     9481 cacggcgcgc agccgggctt cggcctggcc gacatcggca cgcgcggttt cgcaggcagc
     9541 gcgccgcgcc tttgcctcgg tgcgcgcctg gtcctcgcga ccggccgaga cgaagccctc
     9601 gcgctgcagt tgtgcctgcc gttcggcctc gcgcccggcg ttgtcggcaa ccgtgcagat
     9661 ctcgtcgatg cgctgccggg tcaccatcag ctgcgcttcg gcgagcgtcg cctgcgcctg
     9721 ctggtcctcg ttccacaggc gcatcaacac ctggccctgc gcgacccgat cgccttcctt
     9781 gaccagcaag gcatcgatgc ggccgccgag gatcgtcgac aggcgtgtgc gctggcagga
     9841 ttccagcgtg ccggcacggg tattggagag ggtcgattcc acccggccgc ggtcggcagc
     9901 ggcgacgacg accttgattg gctcgggacg ggtcagaaag gcaatggccg cgaccagtgc
     9961 ggcgatgatc acgatgccta gcagcagccg tttgacgatc ggtttcacaa ggcgatccca
    10021 atggaattgg cgcaagcgaa tagtctatca gggcatgctg atgcgccagc cacgatgcgg
    10081 gttttttcga cctgcggcgg catgtgccgc tgacggctcg cgtacactga cggtccaaca
    10141 gcgattcaaa ggcatgacca tgggcgattc gtccgacaac aagcccggca aactttctct
    10201 gccgacgcgt gccgagggcg acgcgccggc gcgaccgggc ggccgccgtg cgccggtccg
    10261 cagcagcggg gtcaggcgtc cacccggccc gccggccgaa aagggcagtg gcgcgccgcc
    10321 acgcggcgaa ggacgtggcg gcgatcgtcc gccccaccgg gaaggcggcc cgcggcccac
    10381 cgagggcgct gcccccgggc ggcgctttga tcgtggcgag cggcccctcg gccgcagtga
    10441 gcaggagcgc gacccacgcc gcgaggggcg gcccggcgat cgtccgcggc caccgcgaga
    10501 cggtgcgccg cgttttgacc gcgagcggcc agctaaaccc tacgaccgtg gcgaccgacc
    10561 gcgccgggac gacagcgatt cgcgcgggcc gcgccgcccg cgcgaaggct tcgtcgggcc
    10621 agcaggtgag ggcgggcgtc cgccgcgcag cggtgagcgg ccacgtttcg aacgtagtga
    10681 tggaggcgcg ggcacggtgg gtgacgcgcc gcgccgcccg cgcgacgaat cgccgcgctg
    10741 gggaagcgat cgacccgcgc gtgccgaggg tgagcccggg cgtggtcccg gtcgctgggg
    10801 cgagcgtcca cggcgacccg acgattcgcg cggcgacagt gatcgtccgc gatttggccg
    10861 tggaccaggc ggtccgcgtg gcggcccagg cggcgatacc ccccggcgcc cccggcgcga
    10921 cgaaggcggc gcgcgcgatg gcgggcgccc ggccgagtcg cggccgcgct ggccgcgtgc
    10981 ggacaggccg ccagcgggcg agcgtccggc gtcagcctgg gcgcgcgatc cggaagcccc
    11041 gtctgccgac tcgaacgaag cgccaagcgc cgagcgcagt ccgcgtcccg ctgccgatac
    11101 ccgcaatgcc gggcgtttcg gtgtcgagca gaccgcgccg cgccccttcg cctggcgtga
    11161 acagaaagtg acggccgcgg attcgccgac gccggccgcg g
//
LOCUS       DATDAA010000399         3186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_204345,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000399 DATDAA010000000
VERSION     DATDAA010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204345"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /gene="metG"
                     /locus_tag="VIS73_12025"
     CDS             <1..1175
                     /gene="metG"
                     /locus_tag="VIS73_12025"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HEY5763926.1"
                     /translation="HALFWPAMLSHAGYRVPTKIFAHGFLTVDGAKMSKSRGTFITAE
                     SYLAQGLDPEWLRYYYAAKLTASMEDLDLALDDFVAKVNSDLVGKYVNIASRSAGFIA
                     KRFAGQLTVCEESLPAIKAIREAAPRIAELYESREFGKAIREIMALTDLANQYVDSVK
                     PWEIAKQAGREGELHAACTNALNLFRLLTILLKPVLPALAGKVADFLKLAPFRWKDLD
                     TVLAAGHAITPYTHLMTRIDPKQIAALVDANRQSLAPQAPAATQPTETKKMTDEKPDA
                     PTISIDDFLKVDLRVARIVDAGVVEGADKLIRLSLDIGETENGEPKLRQVFAGIKSAY
                     DPATLVGRLTVMVANLAPRKMKFGLSEGMVLAASGEEPGLYILSPDSGATPGMRIK"
     gene            1255..2319
                     /locus_tag="VIS73_12030"
     CDS             1255..2319
                     /locus_tag="VIS73_12030"
                     /inference="COORDINATES: protein motif:HMM:NF044596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763927.1"
                     /translation="MDTHSKPIAERIDWLFAHAARHSALFCSPDATLARNRYLARHPT
                     AIAVMKCMDGRINLPVATNTPLGIIQPYRNLGGIFDLGWPHLGEVLAHYVQRRIAEGR
                     RVLFLVTYHFSRGNRERGCAGFHYDTAAAFAHTQHLLGQFETVFGRGHQAVYPLICGF
                     ETDDDALILHGADGATLELASLADATTLPDRLTALYPDMPPQMRDDLLPLLKGNLARI
                     AEVRQTERRLDIEHREWMICLGRGFDFLHMPNLALIVGPYSPQLADPVSTAASIIESN
                     MRAGRIPDDGFLLLASVPYEELGSDRARAELKARFMAGFAAEVIRSRHPALAEKMTVH
                     RAVLDWRSRRLETLPEETSS"
     gene            2316..2600
                     /locus_tag="VIS73_12035"
     CDS             2316..2600
                     /locus_tag="VIS73_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY5763928.1"
                     /translation="MTESIARHLIIEGRVQGVWFRGSMVTEAERLGVAGWVRNRADGC
                     VEALVSGEAMAVQALIDWARRGPPTAKVARVRVEPAEPAPITGFAQKPDD"
     gene            2826..>3186
                     /locus_tag="VIS73_12040"
     CDS             2826..>3186
                     /locus_tag="VIS73_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY5763929.1"
                     /translation="MQKKLIAAAVAALASTAVLADATVYGRLDIGYSSKSAETTGAAK
                     TTTTDTGAMSKLTTSRLGWNVKEDLGGGLSAFGNLELGINALGSAQGASATVAVDSGT
                     AGVPFNTRTAYVGLKSDS"
BASE COUNT          594 a         1152 c          942 g          498 t
ORIGIN      
        1 ttcacgcact gttctggccg gcgatgcttt cgcacgccgg ctatcgcgtg ccgacgaaga
       61 tcttcgcgca cggcttcctc accgtcgacg gcgccaagat gagcaagagc cgtggcacct
      121 ttatcaccgc cgagagctac ctcgcgcagg gactcgaccc cgagtggctg cgctattact
      181 acgcggccaa gctcaccgct tcgatggaag acctcgacct ggcgctcgat gacttcgtcg
      241 ctaaggtcaa cagcgatctg gtcggcaagt acgtcaacat cgcctcgcgc agcgccggct
      301 tcattgccaa gcgcttcgcc gggcaactta ccgtctgcga ggaatcgctg ccggcaatca
      361 aggcgatccg cgaagcggcg ccacggatcg ccgaactcta cgaatcgcgc gaattcggca
      421 aggcgatccg cgaaattatg gcgctcaccg atctggccaa ccagtacgtc gactcggtga
      481 agccttggga aatcgccaag caggcgggcc gcgagggcga actccacgcg gcctgcacca
      541 acgcgctgaa cctcttccgc ctgctgacga tcctgctcaa gccggtactg ccggcgctgg
      601 ccggaaaggt cgccgacttc ctcaagctcg cgccgttccg ctggaaggac ctcgacaccg
      661 tgctcgccgc cggccatgcg atcaccccct acacccacct gatgacccgc atcgacccca
      721 agcagatcgc cgcgctggtc gacgccaacc gccaatcgct tgcgccgcag gcacctgccg
      781 ctacccaacc gaccgaaacg aagaaaatga ccgacgaaaa gcccgacgcc ccgacaatct
      841 ccatcgacga cttcctcaag gtcgacctgc gcgtcgcccg catcgtcgat gccggcgttg
      901 tcgagggcgc cgacaagctg atccgcctct cgctcgacat tggcgaaacc gaaaacggcg
      961 agccgaagct ccgccaggtc ttcgccggca tcaagtcggc ctacgacccg gccacgctgg
     1021 tcggccgtct gacggtgatg gtcgctaacc tcgcgccacg caagatgaag ttcggcctct
     1081 ccgaaggcat ggtgctcgcc gcctcgggtg aagagccagg cctctacatc ctttccccgg
     1141 acagcggcgc gacaccggga atgcgaatca agtaagcgca gcgccaccgc cagccgcggc
     1201 cgccaacttg ccggccgcgc tgcgcttgcc taccatcgct gcgggaggca gggaatggat
     1261 acgcacagca aaccgatcgc cgaacgtatc gactggctgt tcgcgcacgc cgcgcgacac
     1321 tcggcgctgt tctgcagccc cgacgcgacg ctcgcccgca accgttatct ggcacgccac
     1381 ccgacggcca ttgcggtcat gaaatgcatg gacggccgca tcaacctgcc ggtcgctacc
     1441 aacacgccgc tcggcatcat ccagccctat cgaaacctcg gcggcatctt cgacctcggc
     1501 tggccgcacc tcggcgaagt gcttgcccac tacgtacagc gacgcatcgc cgaaggccgc
     1561 cgtgtgctgt tcctggtgac ctaccacttc tcgcgcggca accgcgagcg cggctgtgcc
     1621 ggcttccact acgacaccgc cgccgccttc gcgcacacgc agcacctgct cggccagttc
     1681 gaaaccgtgt tcggcagggg ccaccaagcg gtttacccgc tgatctgcgg cttcgagacc
     1741 gatgacgacg cgttgatcct gcacggcgcc gacggcgcga cgctcgaact cgcatcgttg
     1801 gccgatgcga ccacgctgcc ggatcgactg accgccttgt atcccgacat gccgccgcag
     1861 atgcgcgacg acctgctgcc cttgctcaag ggcaacctcg cccgcatcgc cgaggttcgc
     1921 cagaccgagc gccgcctcga catcgaacac cgcgaatgga tgatctgcct cggccgcggc
     1981 ttcgatttcc tgcacatgcc caacctcgcg ctgatcgtcg gcccctacag cccgcagctc
     2041 gccgaccccg tcagcacagc ggcgagcatc atcgaatcga acatgcgcgc cggccgcatt
     2101 cccgacgacg gcttcctgct cctcgcctcg gtgccctacg aggaactcgg cagcgaccgg
     2161 gcccgagccg agctcaaggc gcgcttcatg gccggcttcg ccgccgaagt catccgcagc
     2221 cgccacccgg cgctcgccga aaagatgact gtccaccgcg ccgtgctcga ctggcgctcg
     2281 cgccggctgg aaacgctgcc cgaggagacc agctcgtgac cgagtcgatt gcccgccacc
     2341 tgattatcga aggccgggtc cagggtgtct ggtttcgcgg cagcatggtc accgaggccg
     2401 agcgccttgg cgttgccggt tgggtgcgca atcgcgccga cggctgcgtc gaggcactcg
     2461 tatcgggcga agcaatggcc gtgcaggcgc tgatcgactg ggcgcggcgc ggcccgccga
     2521 cggccaaagt cgcccgcgtg cgcgtcgaac cggccgagcc cgcgccaatt accggttttg
     2581 cccagaagcc ggacgactga gcgccacact caagggcgcc gaagggcgcg gcgcccgttg
     2641 ttgcgcaaac gcaacatgtg cagcgcaaca taccgaaaat gctggccgtt tgtcttgtat
     2701 cgttatcgca acaggtcaag aatccactcc agcttccggg gcgagccccg aaagtcgggt
     2761 ttcaaggctg taccaaactg tttcacccta tcgggcaaga atgcccacaa cgaggagaaa
     2821 actgcatgca aaagaaactg atcgccgccg ctgttgccgc tctggcttcg accgccgtcc
     2881 tggctgacgc caccgtttac ggtcgtctgg acatcggcta cagcagcaag tcggcggaga
     2941 ccactggcgc cgccaagacg acgacaaccg acacgggcgc gatgagcaag ctgaccacgt
     3001 cgcgcctcgg ctggaacgtc aaggaagacc tcggcggcgg cctcagcgcc ttcggtaacc
     3061 ttgagctggg catcaatgct ctgggttcgg cgcagggcgc gtccgcaacc gtcgcggttg
     3121 acagcggcac ggcaggcgtc ccgttcaaca cccgtactgc ctacgtcggc ctgaagagcg
     3181 acagcg
//
LOCUS       DATDAA010000400         2217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_518417,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000400 DATDAA010000000
VERSION     DATDAA010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518417"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2217
                     /gene="carB"
                     /locus_tag="VIS73_12045"
     CDS             <1..>2217
                     /gene="carB"
                     /locus_tag="VIS73_12045"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY5763930.1"
                     /translation="NPRVSRSSALASKATGFPIAKIAAKLAVGYTLDEIANDITGETL
                     ASFEPTLDYCVVKIPRWTFEKFPETQDILTTAMKSVGETMAIGRTFKEALQKGLRSLE
                     IGRYGLGGDGCDGEPPDRATIEKRLAQPNSQRVFYLRHALRAGMTIAAIHALTAIDPW
                     FLNQIAQLVRFEDSLPAALENLSPDTLRQAKRLGFSDIQLGHLTGSNELRVRERRAAL
                     GLRPVFKLVDTCAAEFEAATPYYYSTYEDENEARVSERRKVMILGGGPNRIGQGIEFD
                     YCCVHASFALRAEGVESIMVNSNPETVSTDYDTSDKLYFEPLTCEDVLHIVETEKPTG
                     VIVQFGGQTPLNLATALAAAGVPILGTQPESIDRAEDREQFQAMLQKLGLTQPANGTA
                     MALEAAAATADAIGYPVIVRPSFVLGGRAMKIVYDKKDLQGFARLAFMASPGHPVLID
                     KFLEDAIEIDVDAIADGERTVIGGIMEHIEAAGVHSGDSACVLPPISLSATLLEEIEG
                     ATRAMAAELKVVGLMNVQYAVKDGRLYVIEVNPRASRTVPFVSKATGVPLAKLATRVM
                     LGRSLEELGLQRQVVPAHVSVKDAVLPFDRFPEVDLLLGPVMKSTGEVMGIDVDFGAA
                     YAKAQLGAGQRLCAGGTVFISVQDRDKAAVLPIAARFAAIGFTIMATGGTAAFLARNG
                     IPNEPINKVSMGRPHVVDAIKNGQIQLVVNTGGGDAPRRDGYTIRRTALKFNVPYVT"
BASE COUNT          414 a          721 c          719 g          363 t
ORIGIN      
        1 aacccccgcg tgtcgcgcag ttcggcgttg gcctccaagg cgaccggctt tccgatcgcc
       61 aagatcgccg ccaaactggc ggtgggctac acgctggacg aaatcgccaa cgacatcacc
      121 ggcgagacgc tggcctcgtt cgaaccgacc ctcgactact gcgtcgtcaa aatcccccgc
      181 tggactttcg agaaatttcc cgagacccag gacatcctga ccacggccat gaaatccgtg
      241 ggtgaaacca tggccatcgg ccggaccttc aaggaggcct tgcagaaagg gctgcgttcc
      301 ttggagatcg gccgctacgg cctgggcggc gacggctgtg acggtgagcc gcccgatcgt
      361 gcgacgatcg aaaaacggct ggcgcaaccc aactcccagc gggtcttcta cctgcgccac
      421 gccctgcgcg ccggcatgac gatcgcggcg atccatgccc tgacggccat cgacccctgg
      481 tttttgaacc agatagcgca gctggtcagg ttcgaagaca gcctgccggc ggccctcgaa
      541 aacctgtcgc ccgacacgct gcgacaggcc aagcggttgg gcttttccga catccagttg
      601 ggtcacctga ccggcagcaa cgagctgcgc gtcagggagc ggcgcgcggc gctgggcctc
      661 cggccggtgt tcaaactggt cgacacctgt gcggccgagt tcgaggccgc cacgccttat
      721 tattacagca cctatgaaga cgagaacgaa gcgcgcgtct ccgagcgccg caaagtcatg
      781 atcctgggcg gtgggcccaa ccgcatcggc cagggcatcg aattcgacta ctgctgcgtg
      841 cacgcttcgt tcgccctgcg ggcggaaggt gtcgaaagca tcatggtcaa cagcaacccg
      901 gagacggtca gtacggacta cgacacctcc gacaagctgt atttcgaacc gctgacgtgc
      961 gaggacgtcc tgcacatcgt cgagaccgaa aaaccgaccg gggtgatcgt gcagttcggt
     1021 gggcagaccc cgctgaatct ggcgaccgct ttggccgccg ccggggtgcc gattttggga
     1081 acccagcccg aaagcatcga ccgggccgag gaccgcgaac agttccaggc gatgctccaa
     1141 aagctcggcc tgacgcagcc ggccaacggg accgccatgg cgctcgaagc ggccgccgcc
     1201 acggccgatg cgatcggcta cccggtcatc gtgcggcctt ccttcgtcct gggcgggcgc
     1261 gccatgaaga tcgtttacga caagaaggac ctgcagggct ttgcacgtct ggcgttcatg
     1321 gcctcgccgg gacacccggt gctgatcgac aaatttctgg aagacgcgat cgaaatcgac
     1381 gtggacgcca tcgccgacgg cgagcgcacc gtcatcgggg gcatcatgga acacatcgaa
     1441 gccgccgggg tgcactccgg ggactcggcc tgcgtgctgc cgccgatcag cctttcggct
     1501 acgcttctgg aggaaatcga gggcgccacc cgggccatgg ccgccgaatt gaaggtcgtc
     1561 ggcctgatga acgtccaata tgcggtcaag gacggccgcc tctacgtaat cgaggtcaat
     1621 ccgcgtgcct cgcgcacggt ccccttcgtc agcaaggcca ccggggtgcc gctggccaag
     1681 ctggccaccc gggtgatgct cggccggagt ttggaggaac tgggtttgca gcgccaggtg
     1741 gtgccggctc acgtgtcggt caaggacgcc gtcctgcctt tcgaccgctt tccggaggtc
     1801 gacctcctgc tcgggccggt gatgaaatcg accggggagg tgatgggcat cgatgtggat
     1861 ttcggcgcgg cctatgccaa ggcccaactg ggcgccggac agcggttgtg cgccgggggc
     1921 acggtgttca tcagcgtgca ggaccgcgac aaggcggcgg tgctgccgat cgccgcacgg
     1981 tttgcggcca tcggctttac gatcatggct accggcggaa cggccgcgtt tttggcgcgc
     2041 aacgggatac ccaatgagcc gatcaacaaa gtgtccatgg ggcggcccca cgtcgtcgac
     2101 gcgatcaaga acggccaaat ccagttggtg gtcaacacgg gcgggggcga cgcgccgcgc
     2161 cgcgacggct acaccatccg acgcacggcg ctgaaattca acgtgcccta cgtgacg
//
LOCUS       DATDAA010000401         5133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_239248,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000401 DATDAA010000000
VERSION     DATDAA010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239248"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2845)
                     /locus_tag="VIS73_12050"
     CDS             complement(<1..2845)
                     /locus_tag="VIS73_12050"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEY5763931.1"
                     /translation="MKNSPQDTPQANEQLALADIPAPPAIAPLPEQDISAEVLIEKYA
                     KGKEASVHDVRRRVARALAAIEIDKERPRWEERFLWAQENGFVPAGRINSAAGTDLAA
                     TLINCFVQPVGDSIVETVEGKPGIYTALAQAAETMRRGGGVGYDFSSIRPVGALVKGT
                     QSRASGPVSYMRVFDRSCETVESAGARRGAQMGVLRCDHPDIEAFVHAKDHGDLTNFN
                     ISIGVTDEFMKAVEADGEVELKHRAEPNADAKSEGAYQRDDGIWVYRKVRARDLWDQV
                     MKSTYDHAEPGILFLDRINRDNNLYYCEEIEATNPCAEQPLPPYGCCCLGSINLTLFV
                     RNSFSDTAEFDFAAFGEVVKVSTRMLDNVLDATHWPLDMQREEAANKRRIGLGYTGLG
                     DALAMLRLRYDRPEARAMAARITEFMRDTAYLTSVELAKERGPFPLFNAELYLSGGNF
                     ASRLPNEVKAEVRKHGLRNSHLLSIAPTGTISLAFADNASNGIEPPFSWYYTRKKRMT
                     DGTIKEYAVEDYAWRLYKHIGGDVERLPDYFVTALEISAKAHEEMVAAVAPYIDTSIS
                     KTVNVPGDYPYADFQDLYIKAWKSGLKGLATYRPNSVLGSVLSVESSKTAEDKKQPQD
                     FVTNDANRRLQIKTLPAPVLASLRWPGRPQFPEGNLAWTYMLNYPHGKVALFVGHLEQ
                     EGKALPFEIWVNGPDVPRGLGAVAKTLSMDMRANDREWLKLKLETLAKTPSSEAFDMA
                     FPPHGELKRMPSIVSALAQVVDFRIEKLGWFAQQGETPVLDAMFSLKEPKTGTDGTLS
                     WTVDVLNPSTGEDFVLGLKEITLPDGVTRPYSVWLAGNYPRALDGLTKLLSLDMRVID
                     PAWIGMKLRKLLNYSEPLGDFMAFVPGTRKQANWPSTVAYVAALMLHRYAMLGILDEA
                     GLPLRQMGILEAPAAGRVRVMQGGQCPECGNHTM"
     gene            complement(3164..4354)
                     /locus_tag="VIS73_12055"
     CDS             complement(3164..4354)
                     /locus_tag="VIS73_12055"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEY5763932.1"
                     /translation="MTQAILTINAGSSSIKFALFALETGEPPAEQAAVVGQVDGIGAR
                     GRLVARDPAGKHEQALGLSGDQAEQHAQAMDFVLRWITEHETGWQITAVGHRVVHGGA
                     IHSAPVLLNDEIVGQLGRFNVLAPLHQPHNLNGIRAISRLLPEVPQVACFDTAFHRTM
                     PPVAELFGLPREITAEGVKRYGFHGLSYEYIARSLPKHLGEKARGRVIVAHLGNGASM
                     AALVAGKSVGSTMGFTAIDGLMMGTRTGNIDPGVLLYLMDNKGMDAKALTDLLYKKSG
                     LLGVSGISQDMRTLLASDAPEAAEAVELFCYRIVRELGSLVAAAGGIDALVFTGGIGE
                     HAAEVRRRVCTQLGWLGVTLDEAANGADATRISAAGSAVAVLVIPTNEEWMIAGHTRE
                     LLAH"
     gene            complement(4351..>5133)
                     /locus_tag="VIS73_12060"
     CDS             complement(4351..>5133)
                     /locus_tag="VIS73_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HEY5763933.1"
                     /translation="KIRSLADELSLDLSTYRIINTKHSHEAAATAVTLARNGDVEALM
                     KGSLHTDELMGEVVARTGGLRTARRISHVFVMDVPTYPRPLLITDAAINIAPTLEDKV
                     HIIQNAIDLAHVMGIAEPKVAILSAVETVNPKIQSTLDAAALCKMADRGQIKGGLLDG
                     PLAFDNAVSLVAAKTKGIRSAVAGQADILVVPDLESGNMVAKQLEYLADALTAGVVLG
                     AAVPIVLTSRADTAETRSASCAIAALMAHAKRKATAAAANAA"
BASE COUNT          804 a         1564 c         1811 g          954 t
ORIGIN      
        1 tcatggtgtg gttgccgcat tccgggcact gcccgccctg catgacgcgc acgcgccccg
       61 cggcgggtgc ttcgaggatt cccatctggc gcagcggcag gccggcctcg tcgaggatgc
      121 cgagcatcgc gtagcggtgc agcatcagtg ccgcgacgta ggccaccgtc gacggccagt
      181 tggcctgctt gcgcgtgccg ggaacgaagg ccatgaagtc gccgagcggc tcagaatagt
      241 tgaggagctt tctcagcttc atcccgatcc acgccgggtc gatgacgcgc atgtcgagcg
      301 agagcagctt agtcaggccg tcgagcgcac gcgggtagtt gccggcgagc cagaccgagt
      361 agggccgtgt gacgccgtcg ggcagcgtga tttccttgag accgagcacg aagtcttcgc
      421 cggtcgaggg gtttagaaca tcgacggtcc acgacagcgt cccgtcggtg ccggtcttcg
      481 gctccttgag gctgaacatc gcgtcgagga ccggggtctc gccctgttgc gcgaaccagc
      541 cgagcttttc gatgcggaag tcgacgacct gtgccagcgc cgagacgatc gagggcatgc
      601 gcttcagttc gccgtgtggc gggaaggcca tgtcgaaggc ttccgacgag ggcgtcttgg
      661 ccagcgtttc gagcttgagc ttgagccact cgcggtcgtt ggcgcgcatg tccatcgaca
      721 acgtcttggc gacggcgccc aggccgcgcg gcacgtccgg gccgttgacc cagatctcga
      781 agggcagcgc cttgccttcc tgctcgagat ggccgacgaa cagcgcgacc ttgccgtgcg
      841 ggtagttaag catgtaggtc cacgcgaggt tgccctcggg gaactgcggc ctgcccggcc
      901 agcgcaggct ggccagtacc ggcgccggaa gcgtcttgat ctgcaggcgg cggttggcgt
      961 cgttggtgac gaagtcctgc ggctgcttct tgtcctcggc ggtcttgctc gattcgaccg
     1021 agagcaccga gccgagcacg ctgttcggcc ggtaggtggc gaggcccttg agtcccgatt
     1081 tccaggcctt gatgtagagg tcctggaagt ctgcgtaggg gtagtcgccg ggaacgttga
     1141 cggtcttcga gattgaggtg tcgatgtagg gggcgaccgc cgctaccatt tcctcgtgcg
     1201 ccttcgccga gatttccagc gcggtgacga agtagtcggg cagacgctcg acgtcgccgc
     1261 cgatgtgctt gtagaggcgc cacgcgtagt cctcgaccgc gtattccttg atcgtgccgt
     1321 cggtcatgcg cttcttgcgc gtgtagtacc aagagaaggg cggttcgatg ccgttgctgg
     1381 cgttgtcggc gaaggccagc gagatcgtcc cggttggcgc gatcgacagc aggtgcgagt
     1441 tgcgcagacc gtgcttgcgg acttcggcct tcacctcgtt gggcaggcgc gacgcgaagt
     1501 tgccgccgga aaggtagagc tcagcattga agagtgggaa ggggccgcgc tctttggcga
     1561 gttcgaccga ggtgaggtag gcggtgtcgc gcatgaattc ggtgatgcgg gcggccatcg
     1621 cgcgggcctc gggacggtcg tagcgcaggc gcagcatcgc cagcgcgtcg ccgaggccgg
     1681 tgtaacccag gccgatgcgg cgcttgttgg cggcttcctc acgctgcatg tcgagcggcc
     1741 agtgcgtcgc gtcgagcacg ttgtcgagca tgcgcgtcga gaccttgacc acttcgccga
     1801 aggccgcgaa gtcgaactcc gcagtgtcgg aaaacgagtt gcggacgaaa agcgtcaggt
     1861 tgatcgaacc caggcagcag cagccgtagg gcggcagcgg ctgttcggcg caggggttgg
     1921 tcgcctcgat ttcctcgcag tagtagaggt tgttgtcgcg attgatgcgg tcgaggaaga
     1981 ggattcccgg ctcggcgtgg tcgtaggtcg acttcatgac ctgatcccac aggtcgcggg
     2041 cgcggacctt gcggtagacc cagatgccgt cgtcgcgctg gtaggcgcct tcgctcttgg
     2101 cgtcggcgtt cggttctgct cggtgcttga gttcgacctc gccgtcggcc tcgacggcct
     2161 tcatgaattc gtcggtgacg ccgatcgaga tgttgaagtt ggtgaggtcg ccgtgatcct
     2221 tggcgtggac gaaagcctca atgtccggat ggtcgcagcg cagcactccc atctgtgcgc
     2281 cgcggcgagc acctgccgat tcgacagtct cgcaggagcg atcgaagacg cgcatgtagg
     2341 agaccgggcc cgaggcgcgc gactgcgtgc ccttgaccag cgcgccgacc gggcggatcg
     2401 aggagaagtc gtagccgacg ccgccaccgc ggcgcatggt ctcggccgcc tgcgccagcg
     2461 ccgtgtagat gccgggtttg ccttcgacgg tctcgacgat cgagtcgccg accggctgca
     2521 cgaagcagtt gatcagggtc gccgccagat cggttccggc agcggagttg atgcggccgg
     2581 cgggtacgaa gccgttttcc tgcgcccaga ggaagcgctc ttcccagcgg gggcgctcct
     2641 tgtcgatctc gatcgcagcc agcgctcgcg cgacgcggcg gcgcacgtca tgaaccgagg
     2701 cctctttgcc cttggcgtac ttctcgatca ggacttcggc ggagatgtcc tgttcaggca
     2761 gcggagcgat ggcgggcggg gcgggaatgt cggccaatgc gagctgttca ttggcctgcg
     2821 gcgtgtcctg cggcgagttt ttcatgcggc tcctcttgtg tactaggtca tggcgcagcc
     2881 tttgcggctg ccagcgtatt tgtgaaaaac acactagata tagtgtgtcg gtgtgtctcc
     2941 ggcactaaat atagttgcag tcccgcctgc aaggcaagga gaaatccgag aaatttctgc
     3001 gcgcctgtgg atagttccgg gacagaccgg ggaaaagctg gggataagaa aaggggcgcg
     3061 aatcgcgccc cttcgtggtg ccgcccctga tggggaaacc ggccaatgtg ttgtgatcga
     3121 gtcacgcctg tggcgcgaac gctacagtgc ccggcgcaat gcctcagtgc gcgagcagct
     3181 cccgggtatg gccggcgatc atccattcct cgttggtcgg aatgaccagt accgcaaccg
     3241 cactaccggc agccgagatg cgcgtggcat cggcgccgtt ggccgcctcg tcgagcgtga
     3301 caccaagcca gcccaattgc gtgcagacgc ggcggcggac ttcggcggcg tgctcgccga
     3361 tgccgccggt gaacaccagg gcgtcgatgc cgccggcggc ggcgaccagc gaaccgagtt
     3421 cgcggacgat gcgatagcag aacagctcga ccgcctcggc agcttcgggc gcgtcgctgg
     3481 ccagcagcgt gcgcatgtcc tggctgattc ccgagacgcc gagcaggccc gacttcttgt
     3541 agagcagatc ggtcaacgcc ttggcgtcca tgcccttgtt gtccatcagg tagagcagca
     3601 cgccgggatc aatgttgccg gtgcgcgtgc ccatcatcag gccgtcgatc gcggtgaagc
     3661 ccatcgtcga gccgacgctc ttgccggcga ccagcgccgc catcgaggcg ccattgccga
     3721 ggtgggcgac gatgacgcgg cccctggcct tttcgcccag atgcttcggc agcgagcggg
     3781 cgatgtattc gtaggagagc ccgtgaaagc cgtagcgttt gacgccttcg gcagtgatct
     3841 cgcgcggcag gccgaacagc tctgcaaccg gcggcatcgt ccggtgaaag gccgtgtcga
     3901 agcaggcgac ctgcggcacc tcgggcagca ggcgcgatat ggcgcggatg ccgttaaggt
     3961 tgtgcggctg gtgcagcggc gcgaggacgt tgaagcggcc gagctggccg acgatttcat
     4021 cgttcagcag gacgggtgcg gaatggatcg cgccgccgtg gacgacgcgg tggccgacgg
     4081 cagtgatctg ccagccggtt tcatgttcgg tgatccagcg cagcacgaag tccatcgcct
     4141 gcgcgtgctg ctcggcctgg tcgccgctca ggccaagggc ctgctcgtgc ttgcccgccg
     4201 ggtccctggc caccagccgg cccctggcgc cgatgccgtc aacctggccg acgaccgccg
     4261 cctgctcggc cggcggctcg ccggtttcga gcgcgaacag cgcaaacttg atcgacgacg
     4321 atccggcgtt gatcgtcagg atggcctgtg tcatgcggcg tttgccgcgg cagcggtggc
     4381 cttgcgctta gcgtgggcca tcagcgcggc aatcgcgcag gaggccgagc gcgtctcggc
     4441 ggtgtcggcc cgcgaggtca gcacgattgg tacggcggcg ccgaggacga cgccagcggt
     4501 cagcgcgtcg gcgaggtatt cgagctgctt ggcgaccatg ttgcccgatt cgaggtccgg
     4561 cacgacgagg atgtcagcct ggccggcaac cgccgagcgg atgcccttgg tcttggccgc
     4621 caccagcgac acggcgttgt cgaaagcgag cggaccgtcg agcaggccgc cctttatctg
     4681 gccacggtcg gccatcttgc acagcgccgc ggcatcgagc gtgctctgta tcttcgggtt
     4741 gacggtttca accgccgaaa ggatcgccac ctttggctcg gcgataccca tcacgtgcgc
     4801 gaggtcgatc gcattctgaa tgatgtgcac cttgtcctcg agcgtcggcg caatgttgat
     4861 cgcggcgtcg gtgatcaaga gcgggcgcgg ataggtcggc acgtccatga cgaagacgtg
     4921 gctaatgcgg cgtgccgtcc gcaggccgcc ggtgcgggcg actacctcgc ccatcagctc
     4981 gtcggtgtgc aggcttccct tcatcagcgc ctcgacgtcg ccgttgcggg cgagcgtgac
     5041 ggccgtcgcg gccgcttcgt ggctgtgctt ggtattgatg atgcggtagg tcgacaggtc
     5101 taggctcagt tcgtcggcga gcgagcggat ctt
//
LOCUS       DATDAA010000402         7675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_448873,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000402 DATDAA010000000
VERSION     DATDAA010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448873"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1267
                     /gene="nuoH"
                     /locus_tag="VIS73_12065"
     CDS             179..1267
                     /gene="nuoH"
                     /locus_tag="VIS73_12065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY5763934.1"
                     /translation="MPAALLDLWTSLPPLAQYLLTVTFKILVVTIGVILVVAFSTYFE
                     RKVIGRIQSRVGPNRVGPLGLLQPFADVFKLLFKEVIVPAKSDRFLFVIAPLLALIPA
                     LATWAVIPLNSWYVIADINAGLLYVLALTSVGVYGVILAGWATNSKYAFLGAMRSAAQ
                     IVAYEIAMGFALVGILMAAGSLNLGDIVEAQSGGIHKWFLFPLLPLFLVYWISGVAET
                     NRAPFDVAEGESEIVAGFHVEYSGVAFALFFLAEYANMVLISSLTAIFFLGGWASPFE
                     GIELLAPIGEIAAIGWVVNGGFPWLFIKMAFFMYTFFWLRATFPRYRYDQIMRLGWKV
                     FIPVTIVWIAVEGAMAWFRIGPWAGLAA"
     gene            1284..1772
                     /gene="nuoI"
                     /locus_tag="VIS73_12070"
     CDS             1284..1772
                     /gene="nuoI"
                     /locus_tag="VIS73_12070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY5763935.1"
                     /translation="MNRLVSYIKTFALWELLKGLSVTQRNFFRKSVTLEYPEEKTPQS
                     PRFRGLHALRRYPNGEERCIACKLCEAVCPALAITIESDVSDDGTRRTSRYDIDLFKC
                     IYCGFCEEACPVDAIVETRIHEYHMEAPGENIMTKDKLLAVGDKYDAMISADKAADAK
                     YQ"
     gene            1781..2386
                     /locus_tag="VIS73_12075"
     CDS             1781..2386
                     /locus_tag="VIS73_12075"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY5763936.1"
                     /translation="MTFEAILFYLFAAVLVGAAVGVVTARNPVHAVMFLVLAFFQSAC
                     LWIMAEAEFLGIVLVLVYVGAVMVLFLFVVMMLDIRVEEVRRTMHRYWALGAAVAALM
                     AVEMVQLIWLRGRDPAATSDFVPYPEGYSNTKALGSVLYTEHVYAFEIAAVILLLAIV
                     AAITLTMRKRPGLKVQNISKQVAVKASDRIRIVKMDAEKKQ"
     gene            2383..2688
                     /gene="nuoK"
                     /locus_tag="VIS73_12080"
     CDS             2383..2688
                     /gene="nuoK"
                     /locus_tag="VIS73_12080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY5763937.1"
                     /translation="MIGLHHYLTLAAILFVLSIAGIIINRRNLIVLLMCIELMLLAVN
                     FNFIAFSRYLGDDTGQVFVFFILTVAAAEAAIGLAILVVLFRNRRSINVQELNTMKG"
     gene            2694..4664
                     /gene="nuoL"
                     /locus_tag="VIS73_12085"
     CDS             2694..4664
                     /gene="nuoL"
                     /locus_tag="VIS73_12085"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY5763938.1"
                     /translation="MLNYYLTIVLAPLAAAIIAGIFGKQIGRAGAHWVTIVAVGISMV
                     LSAIVLKNLYWDGYEPENISIYTWAVTDGLRMEVGFLVDRLTALMMVVVTFVSFAVHV
                     YTIGYMHDDPGYQRFFSYICLFTFSMLMLVMSNNFLQLFFGWEAVGLVSYLLIGFWFK
                     RENAIFANLKAFLVNRVGDFGFILGIAAVVAFTGSLDYAEVFGQANSVAAQTIEIFAG
                     KPWNAMTVICVLLFIGAMGKSAQAPLHVWLPDSMAGPTPISALIHAATMVTAGIFMVA
                     RMSPLYELSATALAVVIVIGAITAFFTGLVGIVQNDIKRVVAYSTLSQLGYMTVALGA
                     SAYSVAIFHLMTHAFFKALLFLAAGSVIIALHHEQDIRKMGGLRKYMPITYWTSLIGT
                     LALIGFPGTSGFFSKDLLIEAVKVSHWQGDGAVYWIAYLSVLLGVFVTALYSFRMFFL
                     VFHGETRMDKETAADVHETPWVVTGPLILLAIPAVVIGWLTVESILFGGFFGDAMVVR
                     QGIDVFAKLEQTFWHGPAALVRHSVVTPVFWLAAAGALTAWFLYLKRPGIPARIKERC
                     LPLYNLLDRKYYMDDLYINGFAAWGRGIGNFLWKKGDELVIDGVIVNGTANSIGKLAA
                     VMRQIQTGYLYTYALAMIIGLTMLLTWLIWFR"
     gene            4681..6195
                     /locus_tag="VIS73_12090"
     CDS             4681..6195
                     /locus_tag="VIS73_12090"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY5763939.1"
                     /translation="MLTQNLLSLLIWLPIAGGVALLVIGDDNDVKSSRAAAMRMTALV
                     TSLLVFVLSIGLYAGFDMTTASMQFVERSSWVPAIDAWYYLGVDGISAPLILLTTFIT
                     PLVVIAGWDTIKVRPAQYFAAFLVLEGLMVGVFSALDGVLFYVFWEAMLIPMFLIIGV
                     WGGARRIYATLKFFLYTFLGSVLTLVAFIYLYGQTGTFDLQTWMAAPLGLTAQKYIFI
                     GFLLAFAVKVPMWPVHTWLPDAHVEAPTGGSVILAAIMLKMGGYGFVRLSLPIVPDGS
                     EYFAGLVIALSLIAVAYIGFVALMQKDMKKLIAYSSIAHMGFVTLGFFLYWYISGSGS
                     VLGIQGGMVQMVSHGLISGALFLCVGVLYDRVHSRQIADYGGVANTMPIFASFMVLFA
                     MANAGLPGTSGFVGEFMVIIASFKANFWFAFVAATTLVLGAAYTLWMVKRVVYGEVAN
                     EKVEALEDLNARELIVLGVLAVSVLLLGLWPAPLVEMMDASIEQLVQQLGQSKL"
     gene            6117..7652
                     /gene="nuoN"
                     /locus_tag="VIS73_12095"
     CDS             6117..7652
                     /gene="nuoN"
                     /locus_tag="VIS73_12095"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEY5763940.1"
                     /translation="MARTAGGNDGCEHRTTRSAARAEQTMMSYIDFPFPFWFVAAPEI
                     VLLTLICVVLIADLFIDQERRIWTFWLSMAALAITMAVVFMTGPYRQMDVMDGAYISD
                     PLSHVLKISAIGFVGLAFFYARDYLRANELHKGEFYLLGLFGLLGIMIMISANSLLIM
                     YLGLETMALSIYALVAIDRNNVVSAESAMKYFVLGAIASGALLYGISWVYGVTGTLRF
                     DEIAAALAADPGLNNLSLWFGLAFLIVGIAFKFGAVPFHMWLPDVYEGARSPVTLYIA
                     TAPKLAALVFALRILVDALGGLHDVWDDMIMVVAVLSLLAGNVIAIAQTNIKRMLGYS
                     TIGHVGFILLGIFCGTDKGYASSLFYTITYIVAAAGAFGIVILLSRRGFEAENLGDFK
                     GLNARSPWFALMMMFILFSLAGVPPFIGFFGKLNVIDATLSAGYPGLAVLMVLASVVG
                     AYYYLRVIWYMYFEEAEDQAVLQASTDTRVIMSLNGAATLALGIAPGWLWALCTAVIA
                     ASS"
BASE COUNT         1203 a         2399 c         2399 g         1674 t
ORIGIN      
        1 acatcgcggg cgacaacacg atcgactgcg agggcagcct gaagggttcg aaagccgccg
       61 acgacctggc gcgggagatc gatacgccga tctactcggt ggacagcctc gtgaggcgcg
      121 ccagggcgct gcagctcaca ccggcagcca ggcgtgtggc cggggagggc ggcgactgat
      181 gcctgctgct ctgctcgatc tctggacctc gctgcccccg ctggcgcaat acctcctgac
      241 ggtcacgttc aagatcctgg tcgtcaccat cggcgtgatt ctcgtcgtgg cgttctcgac
      301 ctacttcgag cgcaaggtca tcggcagaat ccagtcccgg gtcggtccca accgggtcgg
      361 gccgctcggc cttttgcagc ccttcgccga cgtcttcaag ctgctgttca aggaggtcat
      421 cgtcccggcg aagtccgacc gtttcctgtt cgtgatcgcg cctttgctgg cgctgatccc
      481 ggcgctggcg acctgggccg tgatcccgct gaacagctgg tacgtcatcg ccgatatcaa
      541 cgccggtctt ctttacgtcc tggcgctgac atcggtcggg gtttatggcg tcatcctggc
      601 cggctgggcg accaactcga agtacgcgtt cctcggcgcc atgcgctcgg cggcgcagat
      661 cgtcgcgtac gagatcgcga tggggttcgc gctggtcggc atcctgatgg cggccggcag
      721 cctgaatctc ggcgacatcg tcgaagctca gtccgggggc atccataagt ggttcctgtt
      781 cccgctgctg ccgctgttcc tggtctactg gatctcgggc gtggcggaga ccaatcgtgc
      841 gcctttcgac gttgcagaag gcgagtcgga aatcgttgcc ggtttccacg tcgaatactc
      901 tggcgtcgct ttcgccctgt tcttcctggc cgagtacgcc aacatggtgt tgatctcgtc
      961 cctgacggcg atcttcttcc tcggcggctg ggcctcgccg ttcgagggca tcgagctgct
     1021 cgcgcccatc ggcgagattg ctgcgatcgg ctgggtggtc aacggcggct tcccgtggct
     1081 gttcatcaag atggccttct tcatgtacac gttcttctgg ctgcgcgcga cgttcccgcg
     1141 ctaccgctac gaccagatca tgcggctggg ctggaaggtc ttcattcccg tgacgatcgt
     1201 ctggatcgcc gtggaaggcg ccatggcctg gttccggatc gggccctggg cggggctggc
     1261 agcgtaggag cgggcaaaac accatgaatc gactcgtcag ctacataaag accttcgcgc
     1321 tctgggagct tctcaagggc ctgtcggtga cgcagcgcaa cttcttccga aagagcgtca
     1381 cgttggagta ccccgaggag aagacgccgc agtcgccgcg cttccgcggg cttcacgcac
     1441 tgcgccgcta cccgaacggc gaggagcgct gcatcgcctg caagctttgt gaggccgtgt
     1501 gcccggcgct cgcgatcacg atcgagtcag acgtcagcga cgacggcacg cgccgcacga
     1561 gccgttacga catcgatctc ttcaagtgca tctattgcgg tttttgcgag gaggcctgtc
     1621 cggtcgacgc catcgtcgag acgcgcatcc acgagtacca catggaagcg cccggcgaga
     1681 acatcatgac caaggacaag ctgctggcgg tcggcgacaa gtacgacgcg atgatctcgg
     1741 ccgacaaggc ggcagacgcg aaataccagt aggcgtagcg atgacattcg aggcgatact
     1801 gttttatctc tttgcggccg tgctggtcgg cgccgccgtg ggcgtcgtaa ccgctcgcaa
     1861 cccggtccac gccgtcatgt tcctggtgct ggccttcttc cagtcggcct gcctgtggat
     1921 catggccgag gccgagttcc tcggcatcgt gctggtgctc gtctacgtcg gcgcggtaat
     1981 ggtgctgttc ctgttcgtcg taatgatgct cgacatccgc gtcgaggagg tcaggcgcac
     2041 gatgcaccgc tactgggcac tgggggccgc ggtagccgcg ctgatggcgg tcgagatggt
     2101 gcagttgatc tggctgcgcg ggcgcgaccc cgccgcaacc agcgactttg tcccgtatcc
     2161 cgagggctac agcaatacca aggcactcgg ctcggtgctt tacaccgagc acgtctacgc
     2221 cttcgagatc gccgccgtca tcctgctgct cgcgatcgtc gcggctatca cgctgacgat
     2281 gcgcaagcgg cccgggctca aggtgcagaa catatcgaag caggtcgccg tcaaggccag
     2341 cgaccggata cgcatcgtca agatggacgc ggagaagaag caatgatcgg gctgcaccac
     2401 tacctgacgc tggccgcgat cctgttcgtg ctgagtatcg cgggcatcat catcaaccgc
     2461 cgtaacctga tcgtgctgct gatgtgcatc gagttgatgc tgcttgcggt caacttcaat
     2521 ttcatcgcct tctcgcgcta cctcggtgat gacacgggcc aggtgttcgt gttcttcatc
     2581 ctgacggtcg cggccgcgga ggccgccatc ggtctggcca tcctcgtcgt gttgttccgc
     2641 aaccggcgct ccatcaacgt gcaagaactc aacacgatga agggctagcc ggcatgctga
     2701 actactacct caccatcgta ttggcgccgc tggccgcggc gatcatcgcc ggcatcttcg
     2761 gcaagcaaat cggccgtgca ggcgcccact gggtgacgat tgtcgcggtc ggcatctcga
     2821 tggtgctgtc cgcgatcgtg ctcaagaacc tgtactggga cggctacgag cccgagaaca
     2881 tcagcatcta cacctgggcg gtgaccgacg gcttgcgcat ggaggttggc tttctcgtcg
     2941 accgcctgac ggctctcatg atggtggtgg tcaccttcgt ctcgttcgcc gtgcacgtct
     3001 acacgatcgg ctacatgcac gacgacccgg gctaccagcg cttcttcagc tacatctgtt
     3061 tgttcacgtt ctcgatgctc atgctcgtga tgtcgaacaa cttcctgcag ctgttcttcg
     3121 gctgggaggc ggtgggtctc gtgtcctatt tgctgatcgg cttctggttc aagcgcgaaa
     3181 acgcgatttt cgccaacctc aaggccttcc tggtcaaccg cgtcggcgac ttcggcttca
     3241 tcctcggcat tgcggccgtg gttgccttca ccggctctct cgactatgca gaggtcttcg
     3301 gccaggcgaa ctcggtggcg gcccagacca tcgagatctt cgccggtaag ccgtggaacg
     3361 cgatgaccgt gatctgcgtg ctgctcttca tcggcgccat gggcaagtcg gcgcaggcgc
     3421 cgctgcacgt gtggctgccc gactcgatgg ccggcccgac gccgatctcg gccctgatcc
     3481 atgcggcgac catggtcacg gccggtatct tcatggtggc gcgcatgtcg ccgctctacg
     3541 agctgtcggc gacggcgctc gcggtcgtca tcgtgatcgg cgcgatcacg gccttcttca
     3601 cgggtctcgt cggcatcgtg cagaacgata tcaagcgcgt cgtagcctac tcgacgttgt
     3661 cccagctggg ctatatgacc gttgcgcttg gcgcatccgc ctacagcgtc gcgatcttcc
     3721 acctgatgac ccacgccttc ttcaaagcgc tgctgttcct cgccgcaggc tcggtcatca
     3781 tcgcgctgca ccacgagcag gacatccgca agatgggcgg gctgcgcaag tacatgccga
     3841 tcacctactg gacatcgctg attggcacgc tggcgctgat cggcttcccg ggcacgagcg
     3901 gatttttctc caaggacctc ttgatcgagg cggtcaaggt gtcgcactgg cagggcgacg
     3961 gagccgtcta ctggattgcc tacctgagcg tgttgctcgg cgtattcgtg acggcgctgt
     4021 actcgttccg gatgttcttc ctcgtcttcc acggtgagac gcgcatggac aaggagacgg
     4081 ccgccgacgt ccacgagacg ccctgggtcg tgaccgggcc acttatcctg ctggccatac
     4141 cggccgtcgt catcggctgg ctgacggtcg agtcgatcct gtttggtggc ttcttcggcg
     4201 atgcgatggt cgtccggcag ggcatcgacg tcttcgcgaa gctggagcag acgttctggc
     4261 atggtccggc ggcgctggtg cggcactcgg tggtcacgcc ggtcttctgg ctggccgccg
     4321 cgggcgcgct gacggcctgg ttcctgtacc tcaagcggcc aggtattccg gcaaggatca
     4381 aggagcgctg cttgccgctc tacaacctgc tggatcgcaa gtactacatg gacgacctgt
     4441 acatcaacgg tttcgctgcc tggggtcgcg gcatcggcaa tttcctctgg aagaagggcg
     4501 acgagctggt catcgacggc gtcatcgtca acggcaccgc caactccatc ggtaagctcg
     4561 cggccgtcat gcgccagatc cagacgggct acctctacac ctatgcgcta gcgatgatca
     4621 tcgggctgac gatgctcctc acatggctga tctggttcag gtagggcagg ggcgacgacg
     4681 atgctgacgc agaacctgtt gagtctcctg atctggctac cgattgccgg cggcgtggcg
     4741 ttgctggtca tcggcgacga caatgacgtc aaatcgtcgc gcgctgcggc catgcgcatg
     4801 acagcgctcg tcacgagcct gctggtgttc gtgctgagca tcggcctcta cgcgggcttc
     4861 gacatgacga ccgcctcgat gcagttcgtc gagcggtcgt cctgggtccc ggccatcgat
     4921 gcctggtact acctgggcgt ggacggcatc tcggccccgc tgatcctgct gacgaccttc
     4981 atcacgccgc tcgtggtgat cgccggatgg gacaccatca aggtcaggcc ggcgcagtat
     5041 ttcgcggcct tcctggttct cgagggcctg atggtcggcg tgttctcggc gctcgacggg
     5101 gtcctgttct acgtattctg ggaagccatg ctgatcccga tgttcctgat catcggcgtc
     5161 tggggcggcg cgcggcgcat ctatgcgacg ctgaaattct tcctgtacac gttcctcggc
     5221 tcggtgctga cgctggtcgc gttcatctac ctttacgggc agacgggtac cttcgacctg
     5281 cagacctgga tggctgcgcc gctcgggctg accgcgcaga agtacatttt catcggtttc
     5341 ctgctggcct ttgccgtcaa ggtgccgatg tggccggtgc acacctggct gccggacgcc
     5401 catgtcgagg cgccgacggg cggctcggtg atcctggccg ccatcatgct gaagatgggc
     5461 ggctacggtt tcgtcaggct gtcgctgccg atcgtgccgg atggcagcga gtactttgcc
     5521 ggcctcgtga tcgcgctgtc gctgatcgcc gtggcctaca tcggcttcgt cgcgctgatg
     5581 cagaaggaca tgaagaagct gatcgcctat tcctcgatcg cgcacatggg cttcgtgacc
     5641 ctcggctttt tcctctactg gtacatcagc ggctccggct ccgttctcgg catccagggc
     5701 ggcatggtac agatggtctc ccacgggctg atctcgggcg ccctgttcct ctgcgtgggc
     5761 gtgctttatg accgggtcca cagccggcag atcgccgatt acggcggcgt cgccaacacg
     5821 atgccgatct tcgcctcatt catggtgctg ttcgcgatgg ctaacgcggg cctgccgggc
     5881 acctccgggt tcgtcggcga gttcatggtg atcatcgcca gcttcaaggc caacttctgg
     5941 ttcgcgttcg tggccgctac caccctcgtt ctcggtgccg cctatacgct ctggatggtg
     6001 aagcgtgtgg tctatggcga agtcgccaac gagaaggtgg aagcgctcga ggacctcaat
     6061 gcgcgcgaac tgatcgtgct cggcgttctc gcagtctcgg tactgctgct cggcctgtgg
     6121 cccgcaccgc tggtggaaat gatggatgcg agcatcgaac aactcgttca gcagctaggg
     6181 cagagcaaac tatgatgtcc tacatcgact ttccgttccc gttctggttt gtcgccgcac
     6241 cggaaatcgt gctgctgacg ttgatctgcg tggttctcat tgccgacctt ttcatcgacc
     6301 aggagcgccg aatctggacc ttctggctca gcatggcggc gctggcgatt actatggccg
     6361 tggtgttcat gacaggcccc taccgacaga tggatgtcat ggacggcgcc tacatcagcg
     6421 atccgctgtc gcacgtcctc aagatttcgg cgatagggtt tgtcgggctg gcattcttct
     6481 atgcgcgcga ctacttgcgt gccaacgaac tccacaaggg cgagttctac ctgctcggac
     6541 tcttcggcct gctgggcatc atgatcatga tctcggccaa cagtttgctg atcatgtacc
     6601 tcggtctcga gaccatggcg ctatcgatct acgccctggt ggcgatcgac cgcaataacg
     6661 tggtttcggc cgaatcagcg atgaagtact tcgtgctggg ggccatcgcc tcgggcgcgc
     6721 tgctgtacgg catctcctgg gtgtacggag tgaccgggac gctgcgcttc gatgagattg
     6781 ccgcggcgct ggcagcggac ccggggctta acaacctgtc gctgtggttc ggccttgcat
     6841 tcctgatcgt tggtatcgcg ttcaagttcg gcgccgtgcc gttccacatg tggctgcccg
     6901 acgtttacga gggcgctcgc tcgcccgtga cgctctatat cgcgacggcg cccaagctgg
     6961 cggcgctggt atttgcgctg cgtattctcg tcgacgcgct gggcggcctg cacgatgtct
     7021 gggacgacat gatcatggtc gtggcagtcc tgtcgctgct ggccggcaac gtgatcgcga
     7081 tagcccagac caacatcaag cgcatgctgg gctattccac gatcggacac gtcggcttca
     7141 tcctgctggg tatcttctgc ggcaccgaca agggctatgc ctcgtcgctg ttctacacga
     7201 tcacctatat cgtcgcggca gccggcgcgt tcggcatcgt gatcctgctc agcaggcggg
     7261 gtttcgaagc cgagaacctg ggggacttca agggcctgaa cgcgcgcagc ccctggtttg
     7321 cgctgatgat gatgttcatc ctgttcagcc tggccggtgt gcctccgttc atcggtttct
     7381 tcggcaagct gaacgtcatc gatgcgaccc tcagcgccgg ctaccccggt cttgccgtgt
     7441 tgatggtgct ggcctcggtc gtcggtgcct actactacct gcgggttatc tggtacatgt
     7501 acttcgagga ggccgaggac caggcggtgc tgcaggccag caccgacacc cgcgtcatca
     7561 tgagcctgaa cggtgcagcg acgctcgcgc tgggcatcgc cccgggctgg ctctgggcct
     7621 tgtgtacggc cgttatcgcc gcatcatctt aggaacggcc tcagtcacgc caggc
//
LOCUS       DATDAA010000403         3664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_273517,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000403 DATDAA010000000
VERSION     DATDAA010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273517"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="VIS73_12100"
     CDS             <1..1230
                     /locus_tag="VIS73_12100"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEY5763941.1"
                     /translation="GKGHGSDVAILLGLQGETPEAVDTDAVAGIVAAIRARQQIVLLG
                     SHPVAFDEAEHLRLHKRKALPYHPNGMRFEALDAAGAALDSRVYFSVGGGFVVDEAAI
                     DAGFVPPSVAALRHSFATAAELLEHCRTQGKPVSRIMLENELAWRDESTIRADLLHLW
                     AVMQDCVRRGCAGQGVLPGGMKVRRRAADLYRQLRDSQEAALRDPLTVLDWVNLYALA
                     VNEENAAGGRVVTAPTNGAAGIVPAVLHYYARFVPGASDDGIVRFLLTAGAIGILFKL
                     NASISGAEVGCQGEVGSACSMAAGALAEVLGGTPEQVENAAEIGMEHNLGLTCDPVGG
                     LVQVPCIERNAMASIKAINAARIALRGDGRHFVSLDRVIRTMRDTGRDMHTKYKETAR
                     GGLALNVVEVPVNFVEC"
     gene            complement(1237..1779)
                     /locus_tag="VIS73_12105"
     CDS             complement(1237..1779)
                     /locus_tag="VIS73_12105"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY5763942.1"
                     /translation="MSLPVSDPVLLVSGAIVIAWLSRKSLRKRGSHGYYRFFAAEILL
                     VLVVLNRHSSGDQTIANGFLYTSAGLLILGYGALHRFGRASKAREDEALLGFEKTTDL
                     VTGGVFRFIRHPMYASLLALTWGFFFRVPSTLAFSLAAAVSYLLYLTARADERECLAY
                     FGQPYADYMGRTRRFLPFLF"
     gene            complement(1808..>3664)
                     /locus_tag="VIS73_12110"
     CDS             complement(1808..>3664)
                     /locus_tag="VIS73_12110"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5763943.1"
                     /translation="MLSSIGRLDSHRVRTGRLTDDEWSRLSFALGKMHEKPLYIDESG
                     ALNPIELRGRARRLHRQCGKLGLVVVDYLQLMTTTRQGENRATEVSDISRALKSLARE
                     LDVPVIALSQLSRKVEERNDKRPLMSDLRECVVGETLVLLTDGRRVPIRELVGTTPEV
                     WAVDARQKVVAARADKVWSVGRKPVFDLLLASGRRLTATGEHRVLTGSGWMTLNEIEP
                     GQRVALARSLPATRDADDWPDSHCVLLGHLIGDGSYLSGQPLRYTTGSEENSKAVTAA
                     AEGFGVTVNRHAGRGAWHQLVFSGNGNRWQAAGVNKWLRELGVFDQRSSEKRVPQAVF
                     RLDEAKIALFLRHLWATDGSIHVRGDGQRGSARIYFTSVSRGLAGDVAALLLRLGVVA
                     RIKTLKQASGNPLYNVDVSGHTMQQQFLDTVGAFGPRCEPAKSLRAKLADSGSNPNVD
                     TLPVEIFAAVRAAMRRQGVTTREMANLRGTSYGGSSHFRFAPSRATLSSYAERLKDET
                     LQTWAESDLFWDKVVTVEPAGDAEVFDLTVPGPASWLADGVVTHNSGAIEQDADIIMM
                     MYREEYYKPDTQDKGTAEVIIGKQRNGPTGTVRLTFLGEYTRFESQARAAAR"
BASE COUNT          633 a         1223 c         1193 g          615 t
ORIGIN      
        1 ggcaaggggc acggcagcga cgtcgcgata ctgctcggcc tgcagggcga gacgcccgag
       61 gcggtcgata ccgacgcggt cgctggcatc gtcgcggcta tccgcgcgcg gcagcagatc
      121 gtcctgctcg gcagccatcc ggtggccttt gacgaggccg aacacctgcg cctgcacaag
      181 cgcaaggcgc tgccctatca ccccaacgga atgcgcttcg aagcgctcga cgccgccggc
      241 gcggcactcg acagccgcgt ctacttttcc gtgggcggcg gtttcgtcgt tgacgaggca
      301 gcgatcgacg ccgggttcgt accacccagt gtcgccgcgc tgcgccattc gttcgccact
      361 gctgccgagc tgctcgagca ctgccgcacc cagggcaaac cggtcagccg gatcatgctc
      421 gagaacgaac tcgcctggcg cgacgagtcg acgatccgcg ccgacctgct gcatctctgg
      481 gcggtgatgc aggattgcgt gcggcgcggc tgcgccgggc aaggcgtcct gcccggcggc
      541 atgaaggtgc gccggcgtgc cgccgacctc taccggcagc tgcgcgattc gcaggaggcg
      601 gcgctgcgcg acccactgac ggtactcgac tgggtcaacc tctacgcgct cgccgtcaac
      661 gaggagaacg ccgccggtgg gagggtcgtc accgccccga ccaatggcgc tgcaggcatc
      721 gtgccggccg tccttcacta ctacgctcgc ttcgtcccgg gtgccagcga cgatggcatc
      781 gtgcgcttcc tgctcactgc tggcgcgatc ggcatcctct tcaagctcaa cgcatcgatc
      841 tccggagccg aggttggctg ccagggcgaa gtcggctcgg cgtgttcgat ggcggccggc
      901 gcgctcgccg aggtactcgg cggcacgccc gagcaggtcg agaacgccgc cgagatcggc
      961 atggagcaca acctcgggct gacctgcgac ccggtcggcg gcttggtgca ggtgccctgc
     1021 atcgaacgca atgcgatggc ctcgatcaag gcgatcaacg ccgcccgcat cgcgctgcgc
     1081 ggcgacggtc gccacttcgt ctcgctcgac cgcgtgatcc gcacgatgcg cgacaccggg
     1141 cgcgacatgc acaccaagta caaggaaacc gcccgcggcg gattggccct caacgttgtc
     1201 gaggtgccgg tcaacttcgt cgagtgttga gcgcagctag aagaggaacg gcaggaaacg
     1261 ccgggttcgg cccatgtagt cggcgtatgg ctggccgaag tacgccaggc actcgcgctc
     1321 gtcggcgcgc gcggtcaggt agagcaggta cgaaaccgcg gcagccagcg agaaggccaa
     1381 ggtcgacggc acgcgaaaga agaagcccca agtcagcgcc agcaacgagg catacatcgg
     1441 gtggcggatg aatcggaaga cgccgccggt gacgagatcg gttgtcttct cgaatccgag
     1501 caatgcctcg tcctcgcgcg ccttgctggc gcgcccgaat cgatgcaggg ccccgtagcc
     1561 gaggatcagt agccccgcac tggtgtagag aaagccgttg gcgatcgtct ggtcgccact
     1621 cgagtgccga ttgaggacga ccagcacgag caggatctcc gccgcgaaaa accggtagta
     1681 cccgtggctg ccgcgcttgc gcaggctctt gcgcgacagc caggcgatga cgatggcacc
     1741 gctgaccagc agcaccggat cgctgacagg caagctcacc tccggttacc ccgctcgctg
     1801 cgttggctca gcgcgccgcc gcccgcgcct gactctcgaa gcgcgtgtac tcgccgagga
     1861 aggtcaggcg gacggttccc gtcgggccgt tacgctgctt gccgatgatg acctcggcgg
     1921 tccccttgtc ctgcgtgtcg ggtttgtagt actcctcgcg gtacatcatc atgatgatgt
     1981 ctgcatcctg ctcgatggcg cccgagttgt gcgtgacgac tccatcggca agccaactgg
     2041 cggggccggg aaccgtgaga tcgaacacct cggcatcacc ggccggttcg acggtgacca
     2101 ccttgtccca gaacaggtcc gactcggccc aggtctgcag ggtttcgtcc ttgagtcgtt
     2161 cggcgtacga cgaaagcgtt gcgcgcgacg gtgcgaaacg gaagtggctg gacccgccat
     2221 acgacgtgcc ccgcaagtta gccatttctc gtgtcgtcac cccttgccgg cgcatcgcag
     2281 cgcgaaccgc tgcaaagatc tcgaccggaa gtgtgtcgac gttgggattc gaaccgctgt
     2341 cggcaagctt tgcccgcagg cttttggccg gctcacagcg cggcccgaag gcgccaacgg
     2401 tatcgaggaa ttgctgctgc atcgtgtgtc cggaaacatc gacgttgtag agcgggttac
     2461 ccgatgcctg tttcagtgtc ttaattcgtg caacaacccc caaacgcaat agcagcgccg
     2521 ccacatcgcc ggcgaggccg cgactaacgc tggtgaagta gattcgcgcc gagccacgct
     2581 ggccatcgcc ccgcacgtgg attgatccgt cagtcgccca cagatgccgc aggaacagcg
     2641 cgatcttcgc ttcgtcgagc cgaaagaccg cctgcggaac gcgcttctct gaagaacgtt
     2701 gatcaaagac gcccaactct cgaagccatt tgttgacccc ggcagcctgc cagcggttgc
     2761 cgtttccgga aaagaccaac tgatgccaag cgccgcgccc ggcgtgtcga ttgacagtga
     2821 caccgaatcc ttcggcggca gcggtgactg ccttgctgtt ttcctcggag ccggtcgtgt
     2881 agcgcagggg ttggccggaa aggtagctgc cgtcgccgat cagatgcccg agcaggacgc
     2941 agtgcgagtc cggccagtcg tccgcgtcgc gtgttgccgg caagctgcgc gccagcgcga
     3001 cccgttgacc aggctcgatt tcgttgagcg tcatccagcc gctgccggtc agcacgcgat
     3061 gctcgccagt cgcggtcaat cgccgtccgc tagcgagcag caggtcgaaa accggcttcc
     3121 taccgaccga ccagaccttg tcggcgcgtg cggcaacgac cttctgccgt gcatcgacgg
     3181 cccagacttc gggtgtcgtg cccaccaact cgcggatcgg tacgcgacgg ccgtcggtca
     3241 gcagcacgag cgtctcgccg actacgcatt cgcgcaggtc gctcatcagc ggccgtttgt
     3301 cgttgcgctc ctcgactttg cgcgagagct gcgacagcgc gatcaccggt acgtcgagtt
     3361 cgcgcgccag cgacttgagc gcccgcgaga tgtcggagac ctcggtcgcg cggttctcgc
     3421 cctgccgcgt cgtggtcata agctgcaggt agtcgacaac gaccaggccg agcttgccgc
     3481 actggcgatg cagccgccgt gctctgccac gcagttcgat cggattgagc gcgcccgact
     3541 catcgatgta gagcggcttc tcgtgcatct tgccgagcgc aaaggaaagc cgcgaccact
     3601 cgtcgtcggt cagccggccg gtgcgcacgc ggtgggaatc gaggcgaccg atcgacgaga
     3661 gcat
//
LOCUS       DATDAA010000404         5270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_99261,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000404 DATDAA010000000
VERSION     DATDAA010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99261"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VIS73_12115"
     CDS             <1..845
                     /locus_tag="VIS73_12115"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927113.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY5763944.1"
                     /translation="VPGMFDDAQDVVKAVSNDAAFKARYKIGAVNSINWARVAAQIVY
                     YFKGYFAATEDNDQTVSFAVPSGNFGNICAGHVARMMGLPIHRLILATNENDVLHEFF
                     RTGVYRPRKAAETEVTSSPSMDISKASNFERFVFDLVGRDPAVVKALWAQVDAGGSFS
                     LADSPYWQRIGEFGFASGRSSHAERLATISAVWEKYHVMIDTHTADGLRVAVEQREEG
                     IPMLILETAQPAKFGETIREALGIDPIRPADLADIEQLPQRVVSLPPDSAAIKAFIAK
                     TVDG"
     gene            838..1470
                     /locus_tag="VIS73_12120"
     CDS             838..1470
                     /locus_tag="VIS73_12120"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5763945.1"
                     /translation="MVEPNFHEAMWSKRYRDVGDGFLFGSEANRYLRQHVGALPAKGR
                     VLCVADGEGRNSVWLAQQGFLVTATEISPVAVDKARTHARRAGVEVDYRLQDILDWDA
                     PTDAFDAVVAVFIQFAAQSEQPKLFAQLKAALRPGGVLILLGYTLKQLEYKTGGPPCA
                     EQLYDEALLREHFDDCEVLRLDVYEDELAEGTGHCGRSALIGLIARRPPL"
     gene            complement(1465..1602)
                     /locus_tag="VIS73_12125"
     CDS             complement(1465..1602)
                     /locus_tag="VIS73_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763946.1"
                     /translation="MLFALFYVVAITILVLHFTGFLARHNLEWLVLVLAVATFPAVIY
                     L"
     gene            complement(1737..2606)
                     /gene="nadC"
                     /locus_tag="VIS73_12130"
     CDS             complement(1737..2606)
                     /gene="nadC"
                     /locus_tag="VIS73_12130"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY5763947.1"
                     /translation="MNPSDFPFPLHEVIAANVGAALAEDVGSGDLTASLADPATRRRA
                     TVIAREAAVLCGSAWFDACFARIDPAVSIRWALADGDRVAPGATICHLEGPARSLLTG
                     ERSALNFLQLLSGVATKTAEFVALIAGTRAQIVDTRKTLPGLRVAQKYAVCCGGGGNH
                     RMGLFDAILIKENHIAAAGGIARALERAAEIAASSGGICRFIQIEVENLDQLELALAS
                     GAKMILLDNFKPVQLAEAVARNAGRAVLEASGGIDRQSLLAIAETGVDRISIGAITKD
                     IKALDLSMRIDVN"
     gene            complement(2673..3557)
                     /locus_tag="VIS73_12135"
     CDS             complement(2673..3557)
                     /locus_tag="VIS73_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5763948.1"
                     /translation="MNGSHPLRAVLLVLLALCLFAALDATAKYLAARYPVAWLVWGRY
                     AVHLLFMLIVFGPRLGRSLIATGKPVRQVLRALMLTGVTAFNIAALTTLPLAETTAIL
                     FLAPILVAMLAGVWLGETVGRFRTAMALLGFVGVVLIVKPFGGDAGLDGGGVLYALAA
                     AGCYAVYQLMTRQLSPTENTLTMVFYTALTGTVAMTLGMPWFVTLSIPSWTDALLIAS
                     LGLYGGVGHYLLTLAFRDAPASTLSPLLYVQLLWATLLGAVLFGNLPDLLGVTGMLMI
                     AGGGVAIALRERQRARRP"
     gene            complement(3578..5092)
                     /locus_tag="VIS73_12140"
     CDS             complement(3578..5092)
                     /locus_tag="VIS73_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY5763949.1"
                     /translation="MPIQSIADATTPLLLPEALRQLESVHAADGLMERAGAAACEVAL
                     RLRADDARPVLVVAGPGNNGGDAAVVARLLATRGIGVTLSVIGDAANRPADAAAAFAR
                     LDGSGVLVREGVPENACVGLIIDGLFGIGLTRSLTGKFAEAIAAVSSIVSRNAAPVLA
                     LDCPSGLNAASGEQTGPAVPASVTIAFIAAKPGLYTADGPDLCGEIQIDTLGIPSAEI
                     EALADGRLNELDALDAVLSPRPKNSHKGDFGSLGILGGAPGMTGAAILAGRAALKIGC
                     GRVFVGLRDERIAFDPYQPELMLRNPSGLAKAPLEALVCGPGLGTDKIADALLIDILQ
                     SPLPLVLDADALTLIGADGLAAARLRTRHPFTVITPHPGEAARMLKVPVREIGRDRVA
                     AACELASEFNVWVALKGCGTVIASSSGAWWINTSGNPLLASAGTGDVLAGLVGAFLAQ
                     KHAPGTALRAAVHLHGAAADSLHARGMGMGVAAGELIDEIRSVAAAHLATRASA"
BASE COUNT          958 a         1661 c         1794 g          857 t
ORIGIN      
        1 cggtgccggg catgttcgac gacgcccaag atgtcgtgaa ggcggtttcc aatgatgccg
       61 cgtttaaggc gcgctacaaa atcggcgccg tcaattcgat caactgggcg agggttgcgg
      121 cgcagatcgt ctattacttc aagggctact tcgctgcgac cgaggacaac gatcaaaccg
      181 tgagctttgc cgttccctct ggcaatttcg gcaacatctg cgccggtcac gtcgcgcgca
      241 tgatgggttt gccgatccac cggctgatcc tcgcgaccaa cgagaacgat gtcctccacg
      301 agttcttccg gaccggcgtc tatcgcccac gcaaggcggc cgaaaccgag gtgacatcaa
      361 gcccgtcgat ggatatatcg aaagcgtcaa acttcgagcg tttcgtattc gacctggtcg
      421 gtcgcgaccc ggcggtggtc aaggcgctgt gggcacaggt cgacgctggc ggcagcttca
      481 gcctcgccga ttcgccctat tggcagcgga tcggcgagtt cggttttgcg tcaggccgca
      541 gcagccacgc cgagcgcctg gccacgatca gtgcggtctg ggagaagtac cacgtgatga
      601 tcgacacgca cacggcagac ggcctgcggg tcgctgtcga gcagcgtgag gagggtattc
      661 cgatgctgat tctggaaacc gcgcagcccg cgaaattcgg cgaaacgatc cgggaagcgt
      721 tgggtatcga cccgattcgc cccgccgatc tcgctgacat cgagcagctg ccgcagcgcg
      781 ttgtttcgct gccgccggac agcgccgcga tcaaggcctt catcgccaag accgtcgatg
      841 gttgagccca atttccacga ggcgatgtgg tcgaagcgtt accgcgatgt cggtgacggg
      901 tttctcttcg gcagcgaggc caatcgctac cttcggcagc acgtcggcgc cttgccggcg
      961 aaggggcgag tgctctgcgt tgccgacggg gaaggccgca actcggtgtg gctggcgcag
     1021 cagggtttct tggtcaccgc caccgagatc tcgccggttg ccgtcgacaa ggcgcgcacg
     1081 cacgcgcgtc gcgccggtgt cgaagtcgat taccggcttc aggacattct cgactgggat
     1141 gcgccgaccg atgccttcga tgcggtcgtc gcggtgttca tccagtttgc cgcgcagagc
     1201 gagcagccga agctgttcgc gcagctcaag gcagccctgc gcccgggcgg cgtgttgatc
     1261 ctgctcggct acacactgaa gcagctcgaa tacaagaccg gtggtcctcc ctgcgccgaa
     1321 cagctgtacg acgaggcgtt gctgcgcgag cacttcgacg attgcgaagt gctacggctg
     1381 gatgtctacg aggacgaact ggcggaagga acggggcact gcgggcggtc agcgctcatc
     1441 gggctgatcg cgagacgacc accgctatag atagatgacg gcggggaagg ttgcgaccgc
     1501 aagaaccagc accagccact cgaggttgtg ccgtgcgagg aaaccagtga aatgcagtac
     1561 cagaatcgta atggcaacga cgtagaacag cgcgaatagc attctttccc ccgattgcgg
     1621 cgttacgcgg cgcggcgcct tgagaaaccc tgtgcatttc agcgaatgga ttgccgagaa
     1681 catgattccg cgctgattat agctatgtag tgcgctgttg tcgtcaatag atcgggtcag
     1741 ttgacatcga ttcgcatcga cagatcaagc gcttttatgt ccttcgttat agcgccgatc
     1801 gagatgcgat cgacgcccgt ctcggcaatg gcgagcaggg actggcggtc gatgccacct
     1861 gatgcctcaa gaacggcgcg cccggcattg cgggccaccg cttcggcgag ctgaacgggt
     1921 ttgaagttgt cgagcaggat catcttggcg ccgctggcca aggccagttc gagctggtcg
     1981 aggttttcga cctcgatttg gatgaagcgg cagatgccgc cgcttgacgc ggcgatttcg
     2041 gctgcacgtt cgagcgcccg agcgatcccg ccagcggcgg cgatgtggtt ttccttgatc
     2101 aggatggcgt cgaacagccc catccggtga ttgccgccgc cgccacagca gaccgcatat
     2161 ttctgtgcaa ctcgcaggcc cggcagcgtc ttgcgcgtat cgactatctg cgcacgggtt
     2221 ccggctatca gggcaacgaa ctcggcggtc ttggtggcga cgcctgacag cagctggaga
     2281 aaattcagcg cgctgcgctc acctgtcagc aggctgcgcg ccggcccttc taggtggcag
     2341 atcgtcgcgc cgggcgcgac gcggtcaccg tcggcgaggg cccagcggat cgacacggcc
     2401 gggtcaatcc gggcgaaaca ggcgtcgaac caggccgaac cgcacaggac cgcggcttcc
     2461 cgggcgatga ccgtggcgcg cctgcgggtt gcggggtcgg ccaagctggc tgtgaggtcg
     2521 ccgctgccga cgtcttcggc aagtgcggcg ccgacgttgg cggcgatgac ctcatgcagc
     2581 ggaaacggaa agtcgctcgg attcatttcg gctcagcagc cctgcgcaga aaaagagtgc
     2641 gcattctacg acgcgtggcg ccgatcgccg cgctacggcc tgcgggcacg ctggcgctcg
     2701 cgcagcgcga tcgctacgcc gccgccggcg atcatcagca ttccggtaac gccgagcagg
     2761 tcggggagat tgccaaagag cacggcaccg agcagggtgg cccagagcag ctggacgtaa
     2821 agcaggggcg acagcgtcga cgccggtgcg tcgcggaagg ccaacgtcag caggtagtgg
     2881 ccaacgccac cgtaaagacc cagcgaggcg atcaatagcg catctgtcca cgacggtatc
     2941 gacaacgtga cgaaccacgg catgccgagg gtcatcgcca cagtgccggt cagcgccgtg
     3001 tagaagacca tcgtcagcgt gttctcggtc ggcgacagct ggcgcgtcat caattggtag
     3061 accgcgtagc agcctgccgc cgccagcgcg tagaggacgc cgccgccgtc gagtccggcg
     3121 tcgccgccaa agggcttgac gattagcact acgccaacga agccgagcag cgccatcgcc
     3181 gtccgaaatc gaccgaccgt ctctccgagc cagacgccgg cgagcatggc gacgagaatc
     3241 ggtgcgagga acaggatcgc cgttgtttcg gccagcggca gcgtcgtcag ggcggcgata
     3301 ttgaaggcgg tgacgccggt cagcatcagg gcgcgcagaa cctgccgaac cggcttgccg
     3361 gtggcgatca ggctgcggcc cagacgtggg ccgaagacga tcagcatgaa cagcagatgc
     3421 acggcgtatc gaccccagac cagccaggcg accgggtagc gcgcggcgag gtacttggcc
     3481 gtcgcgtcga gggcggcgaa cagacacagc gccaagagaa cgagcagcac cgcgcgcagc
     3541 ggatgcgatc cgttcatggc gacgtcgggt ccggacttca ggcggaggcg cgcgtcgcga
     3601 gatgcgcggc cgcgacgctg cggatttcgt cgatcaattc gccggcagcg acacccatgc
     3661 ccatgcccct ggcgtggagg ctgtcggcgg cggcgccgtg caggtgaacg gcggcacgga
     3721 gcgccgtccc cggcgcgtgc ttctgggcga gaaaggcacc gaccagaccg gcgaggacgt
     3781 cgccggtgcc cgcgctggcg agcagcgggt tgccgctggt gttgatccac caggcgcccg
     3841 atgacgacgc gatcaccgtg ccgcaaccct tgagcgccac ccaaacgttg aactcactag
     3901 ccagctcaca cgcggccgcg acgcggtcac ggccgatctc gcgaaccggc accttgagca
     3961 tccgcgcggc ttcgcccggg tgcggcgtga tgaccgtaaa cggatgccgc gtgcgcagac
     4021 gggcagcggc caggccgtcg gcgccgatca gcgtcagcgc atcggcgtcg agcaccagcg
     4081 gcagcgggct ttgcaggata tcgatgagca gggcgtcggc gatcttgtcg gtgccgagcc
     4141 cggggccgca gaccagcgct tcgagcggcg ccttcgccaa gccgctcgga ttgcgcagca
     4201 tcaattccgg ctggtacgga tcgaaggcga tccgttcgtc gcgcaggccg acgaagaccc
     4261 ggccgcagcc gatcttgagt gcggcgcgcc cggcgagaat cgcggcgccg gtcatccccg
     4321 gcgcaccgcc aaggatgccg agcgaaccga agtcgccctt gtgactgttt ttcgggcgcg
     4381 gtgacaggac cgcgtcaagc gcgtccagtt cgttcagccg gccatcggca agcgcttcga
     4441 tttcggcaga tggaatgccg agcgtgtcga tttggatttc tccgcaaaga tcggggccgt
     4501 cggctgtgta cagccccggc ttggcggcaa tgaaggcgat cgtcacgctg gccggtacgg
     4561 ccggcccggt ctgctcgccg cttgcggcgt taagcccgga cgggcagtcg agggccagca
     4621 ccggcgccgc gttgcggctg acgatacttg agacggcggc gattgcctcg gcgaacttgc
     4681 cggtcagcga acgggtcagc ccgatgccga aaaggccgtc gatgatcagg cctacgcagg
     4741 cgttttccgg gaccccttcg cgaaccagta cgccgcttcc gtcaagtcgg gcaaaggcgg
     4801 cagcggcgtc ggccggccgg ttggcggcat cgccaatgac ggacagcgtc acgccgatac
     4861 cacgggtggc cagcaggcgg gcaacgactg ccgcatcgcc gccgttgttg cccggaccgg
     4921 cgacgaccag taccggtcgc gcatcatcgg cacgcaggcg cagcgcgact tcgcaggcgg
     4981 cggcaccggc ccgctccatc aggccatcgg cggcgtgcac cgattcgagc tggcgcagcg
     5041 cctccggcag cagcagcggg gtcgtggcat cggcgatcga ttgaatcggc atggcgcaat
     5101 tctaggcgcg attcgcacgc ttcaggccag gactgcgcaa atcggcgaag agatcgaggg
     5161 ccaggcagat cgtgttcagg tgaacggtca cgctgtcctc gatcggcttc gcgtagccac
     5221 cggccatgca gatcgcgacc ggccagtggc gctggcggca ggtttcgata
//
LOCUS       DATDAA010000405         3551 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_449200,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000405 DATDAA010000000
VERSION     DATDAA010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449200"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VIS73_12145"
     CDS             <1..714
                     /locus_tag="VIS73_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY5763950.1"
                     /translation="QADAALARALDLQRRSFVSAAAVDQAQAEADAARAARQAADAAL
                     SHAEIRAPFTGIVADRHADVGDLAAPGVPLLTVYRPGAARAVVQLPSAQAAAIRAVGA
                     ALRATIAIGGDAEPIAASRVQVLPVADAATHVVEVRLELPPDAARRAVPGSAVRATFA
                     VGESASLALPAAAIVHRGGIAAAYVRDSEGRFRLRQLRLGEATADGEIEILAGLVAGE
                     IVALDPVKAGIERRRAAER"
     gene            730..>3551
                     /locus_tag="VIS73_12150"
     CDS             730..>3551
                     /locus_tag="VIS73_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5763951.1"
                     /translation="MGISGRIAAYFQHSAMTPLLAIILLLLGVFATLVTPREEEPQID
                     VTMANVFVPFPGATAQDVESLVSIPAEQVLSRMSGVEHVYSVSRPGMSILTVQFEVGV
                     PNQTAVVRLHETVQSHRDWLRPGLGVGEPIVKPKGIDDVPIVALTFWSKDPDRSGFDL
                     QQVARAAETELKRVPGTRDVSTIGGPDHVIRVAMDVESMNAHGVTAQDIRDALQVSNG
                     LQTAGRLVSGNREILLQTGTYIESAADVRAIVVAVRERKPVYLRDVATITDGPDQPPR
                     YVTHSVDGETWPAVTVQVSKKPGVNAADVAEALTRRVESLRGAVIPEGVEVSVTRNYG
                     ATATDKAQKLIGKLVFATAAVVLLVLLALGRREALIVGVAVTLTLAATLFASWAWGFT
                     LNRVSLFALIFSIGILVDDAIVVVENIHRWQALHPGRPLRELIPGAVDEVGGPTILAT
                     FTVIAALLPMAFVTGLMGPYMSPIPINSSLGMFISLAVAFVVTPWLAAKALGHSGAGH
                     EQESRLDRALGNFFRRVLTPFLDPQTGGRARAMLWGVVALLIAVSMGLAVVKLVVLKM
                     LPFDNKSEFQVVLDMPVGTPVEETARVLGELGAVVARIPEVADFQSYAGTAAPINFNG
                     LVRQYYLRSDPQQGDIQVNLVDKADRQRKSHEIAVSVRERIQDIARANGGNAKVVEVP
                     PGPPVLSPIVAEIYGPDYIGQQRVAKAVRAAFEATPDIVAIDDSVDADAPKTTLHVRQ
                     SKAALLGVAQADIVEVVRIGLSGEDVTPAHNTNSKYEIPVRITLPPQRAGSLDALLKM
                     RVRSQSGAMVPVAELVEVRHGQREKTIYHKDLLPVVYVVGDAGGKLDSPLYGLADIHA
                     AVAGMNLDTIERAGGELEPYFIRQPDDPFAGFSMKWDGELQVTYETFRDMGIAYAVGL
                     VLIYLLVVAQFKSYLVPLIIMAPIPLT"
BASE COUNT          568 a         1212 c         1170 g          601 t
ORIGIN      
        1 caggccgacg cggcactggc gcgggcgctg gatctgcagc gccgcagctt cgtcagcgcc
       61 gccgccgtcg atcaggcgca ggccgaggcc gatgcggccc gggccgcccg gcaggccgcc
      121 gacgcagcgc tgagccacgc ggagatacgc gccccgttta ccggaatcgt cgccgaccgc
      181 cacgccgacg tcggcgattt ggcagcgccc ggtgttccgc tgctgactgt ctaccgtccc
      241 ggcgcggcgc gtgcagtcgt gcagcttccc tccgcgcagg cggccgccat acgcgctgtc
      301 ggcgcagccc tgcgcgcaac gatcgccatt ggcggggatg ccgaaccgat tgccgcttcg
      361 cgcgttcagg tcctgccggt tgccgatgcg gcgacgcacg tcgtcgaggt tcgccttgaa
      421 ctgcccccgg acgcggctcg gcgcgctgtg cccggcagcg ccgtgcgcgc caccttcgca
      481 gtcggcgaga gcgccagcct ggccctgccg gcagcggcga ttgtccaccg gggcggcatc
      541 gcggctgcct acgtgcgcga cagcgaaggc cgtttccgcc tgcgccagct ccggctcggc
      601 gaagcgacag ccgatggcga gatcgaaatc ctcgccggcc tcgtcgccgg tgaaatcgtc
      661 gcgctcgatc cggtgaaggc cggcatcgaa cggcgccgcg ccgccgaacg ctgagcgggc
      721 aaaacggaca tgggtatttc cggacgcatc gcggcttact tccagcactc ggcgatgacg
      781 ccattgctgg cgatcatcct gctgctgctc ggagtcttcg cgacgctggt gacgccgcgc
      841 gaggaagagc cgcagatcga cgtgacgatg gccaacgtct tcgtgccctt ccccggagcg
      901 accgcgcagg acgtcgaatc tttggtctcg attcccgccg agcaagtgct ttcacgaatg
      961 agtggcgtag agcacgtcta ttcggtgtcc cgtccaggga tgagcatact caccgtgcag
     1021 ttcgaagtcg gcgtgcccaa ccagaccgcc gtcgttcgcc tgcacgagac cgtccagtca
     1081 caccgggact ggctgcggcc cggcctcggc gtcggcgaac ccatcgtcaa gccgaagggc
     1141 atcgacgatg tgccgattgt cgcgctgacc ttctggagca aggaccccga tcgatcgggt
     1201 ttcgacctgc agcaagtggc gcgcgcggcc gagaccgaac taaagcgggt ccccggcacg
     1261 cgcgacgttt cgacgatcgg cgggcccgac cacgtcatcc gcgtcgcgat ggatgtcgag
     1321 tcgatgaacg cgcacggcgt taccgcgcaa gacatccgcg acgcgctgca ggtgtccaac
     1381 ggtctccaga ccgccggccg gttggtgtcg ggcaaccgcg aaatcctgct gcagaccggc
     1441 acttatatcg aatcggccgc cgacgtccgg gcgattgtcg tggcggtccg ggaacgcaag
     1501 cccgtctacc tccgcgacgt cgcgaccatc accgacgggc cagaccagcc gccgcgttac
     1561 gtcacgcaca gcgttgacgg cgagacctgg ccggcggtca ccgtgcaggt ttcgaaaaag
     1621 cctggcgtca atgctgccga cgtcgccgaa gcgctgacac gccgcgtcga aagcctcagg
     1681 ggtgcggtga ttcccgaagg cgtcgaggta tcggtgaccc gcaactacgg cgcgaccgcg
     1741 accgacaagg cgcagaagct gatcggcaag ctcgtcttcg ccaccgccgc ggtcgttctg
     1801 ctcgtgctgc tggcgctggg ccgacgcgag gcgctgatcg tcggcgtcgc ggtgacgctg
     1861 acgttggcgg cgaccctgtt cgcgtcgtgg gcctggggtt tcaccctgaa tcgcgtctcg
     1921 ctgtttgcct tgattttctc gatcgggatc ctcgtcgacg acgccatcgt cgtcgtcgag
     1981 aacatccatc gctggcaggc gctgcatccg ggtcgaccgt tgcgcgaact gattccgggc
     2041 gccgtcgacg aagtcggcgg gccgacgatc ctcgccacct ttacggtcat cgccgcgctc
     2101 ctgccgatgg ctttcgtcac cggcctgatg gggccttaca tgagcccgat tccgatcaat
     2161 tcctcgctcg gcatgttcat ctcgctggcc gttgccttcg tcgtcacccc ctggctggcg
     2221 gccaaggcgc tcggccattc gggcgccggt cacgagcagg aaagccgtct cgatcgggcc
     2281 ctggggaatt ttttccgtcg cgtgctgacg ccctttctcg acccgcagac cggtggtcgg
     2341 gcgcgggcga tgctgtgggg cgtggtggcg ttgctgattg cggtatcgat gggactcgcc
     2401 gtggtcaagc tggtagtgct gaagatgttg cccttcgaca acaagagcga attccaggtc
     2461 gtgctcgaca tgccggtcgg cacgccggtc gaggaaacgg cgcgcgtgct cggcgaactc
     2521 ggcgcggtcg tcgccagaat ccccgaggtc gccgatttcc agagctatgc cggtaccgct
     2581 gcaccgatca acttcaacgg cctcgttcgc cagtactacc tgcgcagcga tccgcagcag
     2641 ggagacatcc aggtcaatct ggtcgacaaa gccgatcgcc agcgcaagag ccacgagatc
     2701 gccgtctcgg tgcgcgagcg aatccaggat atcgcgcggg ccaacggcgg caacgccaag
     2761 gtcgtcgaag tgccgccggg gccgccggtg ctctcgccga tcgtcgctga aatctacggg
     2821 ccggactaca tcggccagca gcgcgtcgcc aaggccgttc gcgcggcttt cgaggcgacg
     2881 cccgacatcg tcgcgatcga cgactcggtc gacgccgatg caccgaagac caccctgcac
     2941 gtacgccaaa gcaaggcggc actgctcggg gtggctcagg ccgacatcgt cgaggtggtg
     3001 cgcatcggtc tctcaggcga ggacgtgacg ccggcgcaca acaccaattc gaagtacgag
     3061 atcccggtgc gcatcacgct gccgccgcaa cgcgccggca gcctcgacgc cctgctcaag
     3121 atgcgcgtgc gcagccagtc tggggcgatg gtgccggtcg ccgagctggt cgaggtccgc
     3181 cacggccaac gcgagaagac catctatcac aaggatctgc tgccggtggt atacgtcgtc
     3241 ggcgacgccg gcggcaagct cgactcgccg ctctacggcc ttgctgatat ccacgccgcg
     3301 gttgccggca tgaatctcga cacaatcgaa cgggcgggtg gcgaactcga accctatttc
     3361 atccgccagc ccgacgaccc gttcgccggt ttttcgatga agtgggatgg cgagctgcag
     3421 gtcacctacg agaccttccg cgacatggga atcgcctacg ccgttggcct ggtgctgatc
     3481 tacctgctgg tggtcgcgca gttcaagagc tatctggtgc cgctgatcat catggcgccg
     3541 atcccgctca c
//
LOCUS       DATDAA010000406         3833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_380081,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000406 DATDAA010000000
VERSION     DATDAA010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380081"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VIS73_12155"
     CDS             <1..190
                     /locus_tag="VIS73_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M48"
                     /protein_id="HEY5763952.1"
                     /translation="LDRFDRVLPGDPGIAFLKGVSLDAMGDRQRAAEHYAGFLRAGAQ
                     GDPARFAASRLQAFSAGR"
     gene            275..1546
                     /locus_tag="VIS73_12160"
     CDS             275..1546
                     /locus_tag="VIS73_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5763953.1"
                     /translation="MKLETLAVHAGYSPDPTTKAVAVPIYQTTSYAFDDTQHGADLFD
                     LKVQGNIYTRIMNPTQDVLEQRVAAMEGGIAGLALASGMAAITNSILTIAEAGDNIVT
                     SSTLYGGTYNLFAHTLPQYGIDVRFADYRDPASFEKLIDGKTKAVFCESVGNPLGNIT
                     DFEPLAKIAHKHGVPLIVDNTVPSPYLCRPFEHGADIVVHALTKYLGGHGNSIGGIIV
                     DSGKFPWAEHKTRFKRLNEPDVSYHGVVYTEALGPAAYIGRARVVPLRNMGAAIAPFN
                     SFLILQGIETLALRMDRICDNTLKVAQFLKGHAKVGWVSYAGLPDHADHALVKKYMGG
                     KASGIMTFGVKGGSAGGARFQDALKLVTRLVNIGDCKSLACHPASTTHRQLGPEEMKK
                     AGVSEDMVRLSIGIEHIDDIIADLDQALAKV"
     gene            1644..2033
                     /locus_tag="VIS73_12165"
     CDS             1644..2033
                     /locus_tag="VIS73_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763954.1"
                     /translation="MPIPWLTVLKAVPWTEVISNAPKVANGARKLWDSIGNKQVEAVD
                     GGSDKSAQPMTLPEVEAELARQRAAVADLQQQMQASSELIASLAEQNAQLIARVDENR
                     RRLRQLLVGLLATAALGLAALWMASGT"
     gene            complement(2062..2355)
                     /locus_tag="VIS73_12170"
     CDS             complement(2062..2355)
                     /locus_tag="VIS73_12170"
                     /inference="COORDINATES: protein motif:HMM:NF022786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HEY5763955.1"
                     /translation="MSEEAVPAEFWARADRVIDLANEQVKASTIGKVSASLLYAAARF
                     NAFNVASKAASVDDMKSDRDAAIEYFTGQYRRMFIENLDDYVENFAKYKPQGD"
     gene            complement(2557..2871)
                     /locus_tag="VIS73_12175"
     CDS             complement(2557..2871)
                     /locus_tag="VIS73_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763956.1"
                     /translation="MKAWIAGVAGLLLVGCTTVQFGRDFEIARFDSEVRIGTTDQSGV
                     RALLGAPVSTGISMAGGERLVEWTYYYGDGKLSKLSEAKFKLLQIRFDSAGKVESFNW
                     SE"
     gene            complement(3410..>3833)
                     /locus_tag="VIS73_12180"
     CDS             complement(3410..>3833)
                     /locus_tag="VIS73_12180"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HEY5763957.1"
                     /translation="KEAAIPAVLGLIVAGSALTRAPLVRVLLYTPMLLDTGKIDMALA
                     ERGNLPAFEARLRAATWMLAASFAFSSAMNFALATWIVKSPAGSAAFNEELGRLTLLS
                     YPIIALPAALMMMGVLWYLAIGVKRLAGLRLTDALHGK"
BASE COUNT          764 a         1301 c         1093 g          675 t
ORIGIN      
        1 cctggaccgc ttcgaccgcg tgctgcccgg cgatccggga atcgcattcc tcaaaggcgt
       61 gagtctcgac gccatgggcg atcgtcaacg cgctgctgag cactacgcgg gcttcctgcg
      121 cgccggcgcc cagggtgatc cggcgcgctt cgcggcatcc cggctgcagg cgtttagcgc
      181 cgggcgttaa gcacccgacg cactcagcga cccggcaata cttcaggcgg ggctagaatt
      241 cgtcgctttc aataccaacc agcggagggg atgcatgaaa ctcgaaacac tggccgtaca
      301 cgccggttac agcccggacc cgaccaccaa ggccgtcgcg gtgccgatct accagaccac
      361 ctcgtatgcc ttcgacgaca cgcaacacgg cgccgacctc ttcgacctca aggtccaggg
      421 caacatctac acgcggatca tgaacccgac gcaggacgtg ctcgagcagc gcgttgccgc
      481 gatggaaggc ggcattgccg ggctggcgct ggcctcgggc atggcggcaa tcaccaattc
      541 gatcctgacc atcgccgagg ccggcgacaa catcgtcacc tcgtcgacgc tctacggcgg
      601 cacctacaac ctcttcgccc acacgctgcc gcagtacggc atcgacgtgc gcttcgccga
      661 ctaccgcgat ccggcctcct tcgaaaaact gatcgacggc aagaccaagg cggttttctg
      721 cgaatcggtc ggcaacccgc tcggcaacat caccgacttc gagccgctcg ccaaaattgc
      781 ccacaagcat ggcgtgccgc tgatcgtcga caacacggtg ccctcgccct acctgtgccg
      841 cccctttgag cacggcgccg acatcgtcgt ccacgcgctg accaagtacc tcggcggcca
      901 cggcaactcg attggcggca tcatcgtcga ctcgggcaag tttccctggg cagagcacaa
      961 gacgcgattc aagcgcctca acgaacccga cgtctcctac cacggcgtcg tctacaccga
     1021 ggcactcgga ccggccgcct acatcggccg cgcgcgcgtc gtgccgctgc ggaacatggg
     1081 cgcggccatc gcgccgttca acagtttcct gatcctgcag ggcatcgaga cgctggcgct
     1141 gcgcatggac cgcatctgcg acaacacact gaaggtcgcg cagttcctca agggacacgc
     1201 aaaggtcggc tgggtcagct acgccggcct gcccgatcac gccgaccacg cgctggtcaa
     1261 gaagtacatg ggtggcaagg cctcgggaat catgaccttc ggcgtcaaag gcggcagcgc
     1321 cggcggcgcg cgcttccagg atgccctcaa gctggttacg cgtctggtga acatcggcga
     1381 ctgcaagagc ctcgcctgcc atccggcgag taccacgcac cgccagctcg gccccgagga
     1441 aatgaagaag gcaggcgttt ccgaagacat ggtccgcctg tcgatcggca tcgagcacat
     1501 cgacgacatc attgcggacc tcgaccaggc gctcgccaag gtctgaacct tcgcggcgag
     1561 ctttggccca accgaaacgg cgcatcaccg atgcgccgtt tctccatccg caaagcgcac
     1621 aacccatcac aacaggaacc gacatgccga tcccctggct caccgtcctc aaggccgtcc
     1681 cgtggaccga ggtcatcagt aatgcgccca aggttgccaa cggcgccaga aaactctggg
     1741 attcgatcgg caacaagcag gtagaagctg tcgacggtgg ctctgacaag tccgcgcagc
     1801 caatgaccct gcctgaagtc gaagccgagc ttgcccgcca gcgcgcagcc gtcgccgacc
     1861 tccagcagca gatgcaggcc tcgtccgaac tcatcgcctc gctcgccgag cagaacgcgc
     1921 aactgatcgc ccgcgtcgac gagaatcgcc gacgcctgcg ccagcttctc gtcggcctgc
     1981 ttgctactgc cgcgctcgga ctggcagcct tatggatggc gtctggaacg tgaagcggga
     2041 tgtccggcgg aaccgcctgg ctcaatcgcc ctgcggcttg tacttcgcaa aattctcgac
     2101 atagtcatcg aggttttcga tgaacatcct ccggtactgc ccggtgaagt actcgattgc
     2161 tgcgtcccgg tcgcttttca tgtcatcgac actggccgct ttcgaggcaa cgttgaaggc
     2221 gttgaaacgc gccgccgcat agagcagcga ggcactgacc ttgccgatcg tcgaggcctt
     2281 gacctgctcg ttggcgagat cgatcacgcg atcggctcgc gcccagaact cagccggaac
     2341 agcctcttcg ctcataaccg aacctttcca ttcaagacat tgaacgattg aatcaggccc
     2401 aacaggactc gccgccatcg cgcaccgact tcctgccgtt tcggaagtcc gtggaatttc
     2461 gggaagagcc cgttaaacgc tcggcaagca ggcagctgtt ccaataggac ccagtgggac
     2521 acccggccac gaaaagggcc gcgcctgttc cccttgctac tcggaccagt tgaacgactc
     2581 cacctttccc gcggaatcga aacggatctg cagcagcttg aacttcgctt ctgacagctt
     2641 cgacagcttg ccgtcgccgt agtagtaggt ccactccacc aaacgctcgc ctcctgccat
     2701 cgagatgccc gtggataccg gcgccccgag cagcgcacgg accccgctct ggtctgtggt
     2761 accgattcgc acctcggagt cgaagcgtgc gatctcaaag tcacgaccga actgcaccgt
     2821 ggtgcacccg acgagcaaca agccggcaac gccggcgatc catgccttca tcgatgtccc
     2881 ctcgttagtc gcgccccggc gaggcgcgtt cagtctgaag gatagaagat tcccatttgg
     2941 taggaatcct gccgtgggct tggcctcgcc tatccccact ccggcatcgt gggctggcaa
     3001 cgccgcgcga cagcgacagc gcttgatcca actgcgccat ttcgataacc agatgctcgg
     3061 caattatcat ccgcgtgacg tttgccacag aagtcttcta tgctcgcttc tcggccattc
     3121 cggactttga cgctgcgcgc tttcaccggc acgcagtcat gcgtcctcca gaccactgcg
     3181 ggagacctga cttactcggt ggacagcggc ccgctcaacc gcgacggtga gagctacctg
     3241 ctgctcgtac acccgatgcc ctagggacaa agttggcacc actactcgga acaatctggc
     3301 tgccatatgc gcgtcgttca tcgactaggg ggtatctgcc atgcgtgtgc aaagtacatt
     3361 gcccttctta attctttcag cctgaatcgc ccccgcgctc aatcagtctt tatttgccgt
     3421 gcaatgcatc ggtcagacgc aagccggcaa gacgtttaac accgatcgcc aaataccaaa
     3481 gcacacccat catcatcagc gcggcaggca gggcgatgat ggggtagctc aacagggtca
     3541 gcctgccgag ttcttcgttg aacgcggcag agcccgctgg gcttttgacg atccacgtgg
     3601 ccagtgcgaa gttcatggcc gacgaaaatg cgaaggaggc ggccagcatc caggtggcgg
     3661 cgcgcaggcg ggcctcgaag gcgggcagat tgcctcgttc ggccagagcc atgtcgattt
     3721 tccctgtgtc gagcagcatt ggggtgtaga gcaacacgcg caccaggggc gcgcgcgtga
     3781 gcgcggaacc ggcgacgatc aggcccagca ctgcgggaat cgctgcctcc ttc
//
LOCUS       DATDAA010000407         2297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_518856,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000407 DATDAA010000000
VERSION     DATDAA010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518856"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /gene="lgt"
                     /locus_tag="VIS73_12185"
     CDS             <1..507
                     /gene="lgt"
                     /locus_tag="VIS73_12185"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY5763958.1"
                     /translation="MSFHGGFLGVVLAVWLFARKHGLRFWNVIDAVAPVVTIGLGAGR
                     IGNFINGELWGKETTVPWSFNVNGRALHPSQLYEALLEGLVLFVILWVFSSKKRPAMA
                     VSAMFLIFYGLFRCIVEFYRMPDADKGYVLFGWVTTGQVLSAPMFVAGVIMLYLAYRR
                     DARTEELR"
     gene            509..1303
                     /gene="thyA"
                     /locus_tag="VIS73_12190"
     CDS             509..1303
                     /gene="thyA"
                     /locus_tag="VIS73_12190"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY5763959.1"
                     /translation="MQQYLAMMRHIRETGARKEDRTGTGTLSVFGHQMRFDLGEGFPL
                     VTTKKLHLRSIIHELLWFLSGDSNVRYLRENGVTIWDEWADENGDLGPVYGVQWRRWQ
                     TAAGDTVDQVADVARRLREDPDSRRIIVCAWNVADIPRMALPPCHCLFQFYVADSKLS
                     CQLYQRSADVFLGVPFNIASYALLTHMLAQQADLNVGDFIWTGGDCHLYLNHLEQADE
                     QLSREPLPLPRLAITRKPESIFDYRFEDFEILNYRSHPHIKAAVAV"
     gene            1307..1822
                     /gene="folA"
                     /locus_tag="VIS73_12195"
     CDS             1307..1822
                     /gene="folA"
                     /locus_tag="VIS73_12195"
                     /inference="COORDINATES: protein motif:HMM:NF008037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HEY5763960.1"
                     /translation="MRISIIVATSANNVIGADGGLPWHLPEDLRRFREVTMGKPMIMG
                     RATWDSIGRALPGRRSIVLTRQADFLAEGARVVRSVDEALAAAGDVDEVMVIGGGEIY
                     REFLPRTDRIYLTRLEADIQGDTRFPELDMEQWLVAGAEEYPAGGEREFGFTIEVLDR
                     RADSTGPGDIE"
     gene            complement(1940..>2297)
                     /locus_tag="VIS73_12200"
     CDS             complement(1940..>2297)
                     /locus_tag="VIS73_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763961.1"
                     /translation="AGWDRVFEEFEKAQMANANAIADMALENYVEMRDTVRDPKHVLR
                     HALAFELERRLPTRFVPRYSMVMFHDEIPYATARERGALQRRLLEELTATANSLDDID
                     LDAAVALAESRLPPLR"
BASE COUNT          372 a          754 c          754 g          417 t
ORIGIN      
        1 atgtccttcc acggcggttt cctcggcgta gtgctggcgg tgtggctgtt cgcccgcaag
       61 cacggcctgc gcttctggaa cgtgatcgat gccgtcgccc cggtcgtgac catcggcctc
      121 ggcgccgggc gcatcggcaa tttcatcaac ggcgagctgt ggggcaagga aacgacggtg
      181 ccctggagct tcaacgtcaa cggtcgcgcc ctgcacccgt cgcaactcta cgaggcgcta
      241 ctcgagggcc tggtcctgtt cgtcatcctc tgggtcttct cgtcgaagaa gcgcccggcc
      301 atggcggtat cggccatgtt cctgatcttc tatggcctgt ttcgctgcat cgtcgagttc
      361 taccgcatgc ccgacgccga caagggatac gttctgttcg gctgggtcac gacgggccag
      421 gttctcagcg cgccgatgtt cgtcgccggc gtcataatgc tctacctcgc ctatcggcgt
      481 gatgcgcgca cggaggaatt acgctgatat gcagcagtac ctcgcgatga tgcgccacat
      541 ccgggaaaca ggtgcgcgca aggaggatcg taccggcacg gggacgctga gcgtattcgg
      601 tcaccagatg cgcttcgatc tcggcgaggg ctttcccctg gtgacgacaa agaaactgca
      661 tcttcgctcg atcatccacg aactcctctg gttcctgagc ggcgattcca acgtgcgcta
      721 cctgcgcgaa aacggcgtga ccatctggga cgaatgggcg gacgagaacg gcgatctggg
      781 accggtttac ggcgtgcagt ggcggcgctg gcagacggcc gccggcgata ccgtcgacca
      841 ggttgccgac gtcgccaggc gtctccgcga ggacccggat tcccggcgga tcatcgtctg
      901 tgcgtggaac gtggcggaca tcccgcgcat ggccctgccg ccctgccact gcctgttcca
      961 gttctacgtg gccgacagca agctgtcctg ccagctttac cagcggagcg ctgacgtctt
     1021 cctcggcgtg cccttcaaca ttgcgtctta tgcgttgctg acgcacatgc tggcccagca
     1081 ggccgacctc aatgtcggcg acttcatctg gaccggcggc gactgccacc tttatctcaa
     1141 tcacctcgag caggccgacg agcagctgtc gcgcgagccc ctgccgctgc cgcgactggc
     1201 gatcacgcgc aagccggaga gcatcttcga ttatcgattc gaagacttcg agatccttaa
     1261 ctaccggtcg cacccccaca tcaaggcggc cgtggcggtc tgattcatgc gcatctcgat
     1321 catcgtcgca acgtccgcca ataacgtcat cggcgccgac ggcgggttgc cctggcacct
     1381 gcccgaggat ttgcgccgct tcagggaagt cactatgggc aagccgatga tcatgggccg
     1441 ggcgacctgg gattccatcg gccgcgcgct gccgggccgc cgcagcatcg tcctcacgcg
     1501 gcaggctgac tttctggccg agggcgcccg ggtggtccgc tcggtcgacg aagcgctggc
     1561 cgcggcgggc gacgtcgacg aggtcatggt catcggcggc ggcgagatct accgggagtt
     1621 cctgccgagg acggatcgca tctacctgac gcgcctggaa gcggacatac agggcgatac
     1681 gcgttttccg gagctcgaca tggagcagtg gctggtggcc ggcgccgaag aatacccggc
     1741 gggcggggaa cgcgagttcg gcttcacgat cgaggtgctg gaccgccggg cggactcgac
     1801 cgggcccggc gatatcgagt agcgaagccg gctgggcccg ccgccgcggc cgcggtccgg
     1861 cgcccgggaa ttcgaattcg ttcataacga caccaaccgg gacccggcgc ggcaatccgg
     1921 ctgcccggcg ccgcggcggt caacgaagcg gcggcaggcg cgactccgcg agcgccacgg
     1981 ccgcatcgag gtcgatatcg tccaggctgt tcgcggtcgc ggtcagttcc tccagcagcc
     2041 gccgctgcag cgcgccgcgt tccctggccg tcgcataagg gatctcgtcg tggaacatga
     2101 ccatcgaata gcgcgggacg aagcgcgtgg gcaggcgccg ctccagttcg aaggccaggg
     2161 cgtgccgcag gacgtgttta gggtcgcgga ccgtgtcgcg catctcgacg tagttttcga
     2221 gcgccatgtc ggcgatcgcg ttggcgttgg ccatctgcgc cttttcgaac tcctcgaaaa
     2281 cccggtccca gccggcc
//
LOCUS       DATDAA010000408         2092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_310033,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000408 DATDAA010000000
VERSION     DATDAA010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310033"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1891)
                     /locus_tag="VIS73_12205"
     CDS             complement(<1..1891)
                     /locus_tag="VIS73_12205"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5763962.1"
                     /translation="MKSHAKVVVIGGGVVGVGALYHLAKKGWGEEVVLLEKAELTSGS
                     TWHAAGLLPLFNMSYSVGQIHKYSLRLYRQLEAETGQDVGFRQVGNLRLAMNDDRMDE
                     YYQYAATARTIGVDVRFLTPDDILELWPMCNIEGLVGGIFHPDDGYIQPADLTQALAR
                     GARNMGATIHRKTPALNIEQTSSGAWLVETPSGNITCEHVISATGNYARKTGEMVGLD
                     VPVIPVEHQYIVTEPHPELVRRRDSGAPEMGVLRESDGSWYLREENGGFILGPYEKGA
                     PACYVDGPDPRAEYELFQEDIERLAPHIEAAMNRVPAFAEVGIKQVYNGAIAYTPDGN
                     PIIGPAWDLDNFWLSEGHSFGITAAGGAGWQLAEWIVEGEPTIDMLGVEPRRFGDYAT
                     KKYLVEKNEEAYSHVFIIHYPDEERPACRPLRTAPCYERMKERGAVFGQKFGWERPNF
                     FATDGMPQEDDWSFRRSRWFEAIRKEVMNTTENVGVLDMTAFAKCRIAGPGAEAFLDG
                     FLANSLPARTGGMNLSHALNRNGGIHSEFTVCREAADSFYLVSAGALQRLDHDYIRRY
                     MPRDGSVQFTPLTGAIGVLVVAGPKARQLLQRVTDADLSNEAFRWLSARDIVVGAAPA
                     KAMRVN"
BASE COUNT          320 a          675 c          696 g          401 t
ORIGIN      
        1 agttgacgcg catggccttg gccggcgccg cgccgacgac gatgtcccgc gcgctgagcc
       61 agcggaacgc ctcgttcgac aggtcggcgt cggttacgcg ctgcaacagc tgccgcgcct
      121 tcgggccggc gacgaccagg acgccgatcg cgccggtcag cggggtgaac tgcacggagc
      181 cgtcgcgcgg catgtagcgc ctgatgtagt cgtgatcgag gcgctgcagg gcgccggcgg
      241 acaccaggta gaacgagtcc gccgcttcgc ggcagaccgt gaattccgag tgaatgccgc
      301 cgttccggtt caacgcgtgc gagagattca tgcctcccgt cctggcgggc aggctgttcg
      361 ccaggaaacc gtcgaggaag gcctcggcgc cgggcccggc gatgcggcac ttggcgaagg
      421 cggtcatgtc gagcacgccg acgttctcgg tcgtgttcat gacctccttg cgaatggcct
      481 cgaaccagcg cgagcgccgg aaggaccagt cgtcctcctg gggcatgccg tcggtcgcga
      541 agaaattggg ccgctcccag ccgaatttct ggccgaacac ggcgccgcgc tccttcatgc
      601 gttcatagca cggggccgtc ctcaacgggc ggcacgcggg cctttcttcg tccgggtaat
      661 gaatgatgaa gacgtgcgaa taggcttcct cgtttttctc gacgaggtac tttttcgtgg
      721 cgtagtcgcc gaagcggcgc ggttccacgc cgagcatgtc gatcgtcggc tcgccctcga
      781 cgatccactc ggccagctgc cagccggcgc cgcccgcggc cgtgatgccg aagctgtggc
      841 cctcgctaag ccagaagttg tcgaggtccc aggccgggcc gatgatcggg ttgccgtccg
      901 gcgtataggc aatggcgccg ttgtagacct gcttgatgcc gacctcggca aaggccggga
      961 cccggttcat cgccgcctcg atgtgcggcg cgagccgctc gatgtcttcc tggaacagtt
     1021 cgtactcggc gcggggatcc gggccgtcca cgtagcaggc gggcgcgcct ttctcgtagg
     1081 ggcccaggat gaagccgccg ttttcctcgc gcaggtacca ggagccgtcg gactcgcgga
     1141 gcacgcccat ttccggcgcg ccgctatcgc gccgcctgac cagctcggga tgcggttcgg
     1201 tgacgatgta ctggtgctcg accgggatga ccgggacgtc gaggcccacc atctcgccgg
     1261 ttttccttgc gtagttgccg gtggcgctga tgacgtgctc gcaggtgatg ttgccgctcg
     1321 gcgtttcgac cagccaggcg ccggaggatg tctgctcgat gttgagcgcc ggcgtcttgc
     1381 gatgaatcgt ggcgcccatg ttgcgagcgc cgcgggcgag cgcctgggtc aggtcggccg
     1441 gctggatgta accgtcgtcc gggtgaaata ttccgccgac cagcccctcg atattgcaca
     1501 tgggccacag ctcgagaatg tcgtccggcg tcaggaagcg cacgtcgacg ccgatggtcc
     1561 tggcggtcgc ggcatactgg tagtactcgt ccatgcggtc gtcgttcatc gccagcctga
     1621 ggttgccgac ctggcggaag ccgacgtcct ggccggtttc cgcttcgagc tgcctgtaaa
     1681 gcctgagcga gtacttgtgg atctggccga cgctgtagct catgttgaag agcggcagca
     1741 ggcccgcggc gtgccaggtc gagccggagg tcagctcggc cttctcgagc aacacgacct
     1801 cctctcccca gccttttttc gccaggtgat agagcgcgcc gacgccgacg acgccgccgc
     1861 ctatcacgac cactttagcg tgcgatttca tctgtttcgt tcctgtcgaa tcgtttctta
     1921 ttcttcgcaa gggggagcgg ctaatttatc cgatcgatcc gcctctgccc atttcgagtg
     1981 cacatgctga agcccgcccg tcagaaatac gggcgcggct ccttcgcgaa cagccgcctc
     2041 accatcggct cgaaatgttc gagcggcagc gtgtcgtaat ccgggtcgaa cg
//
LOCUS       DATDAA010000409         2176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_519001,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000409 DATDAA010000000
VERSION     DATDAA010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519001"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VIS73_12210"
     CDS             complement(<1..710)
                     /locus_tag="VIS73_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5763963.1"
                     /translation="MDKNLSRRQFLNLVGAAGGTTAVYQTSRALGLLEDTGPVATLDM
                     KNVADGGKNVAILGAGIAGLTIAYELERAGYRVTVLEASHRAGGRNFTVRHGTVIDEM
                     GNRQVCDFDDDPNLYFNAGPARIPGHHRRTLHYCRILDVPLIVKANASRQAYTHEEDH
                     FGGKPVRVGRYTTDTRGFVAELLYKAVDKNAFEQPLSAEDRERMLVFARDYGDLDAEG
                     LYKGSSRAGYKSGGFGRDA"
     gene            complement(789..1139)
                     /locus_tag="VIS73_12215"
     CDS             complement(789..1139)
                     /locus_tag="VIS73_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763964.1"
                     /translation="MSESLQDSIEAIEAGYEFLLAYAAQGRETDEGPGGSEVRDTLNS
                     MAAAAASIGAALAADGDPFSAVVVEDARKSGAAIALVLAQEAISSELVDNLNASIHLR
                     ALLTDLFLLSETMS"
     gene            complement(1182..1940)
                     /locus_tag="VIS73_12220"
     CDS             complement(1182..1940)
                     /locus_tag="VIS73_12220"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY5763965.1"
                     /translation="MDIASYKNDVWGQEVLRGVSWDLLWLVIVAAFVIIALHAVIEAL
                     RKRAEKPSSEGARVTRHDTIDRAYHWVMAASVFVLLVTGVFPIIGLEFAWLEIHWIAG
                     LVLTIAVVFHIVRAFVAQDWREMSFSGSDLKEPFDDSVKPGKYSAAQKSMHLAVSVLT
                     LLVIVSGLVMFAMIDTPWWERSNALSEAMLGIVFFLHGISTLGLIGLICLHVYFALRP
                     EKLFYTRSMIRGWISRDELAAHHDPQRWTPDEAS"
     gene            complement(1945..>2176)
                     /locus_tag="VIS73_12225"
     CDS             complement(1945..>2176)
                     /locus_tag="VIS73_12225"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763966.1"
                     /translation="MSMDGDTVADSRIVCGGVACTPYRLRSVERALRGKPLSGELADS
                     VAPLASKGAKPLNYNHFKLPLMENLVRRAMRS"
BASE COUNT          379 a          704 c          686 g          407 t
ORIGIN      
        1 gcatccctgc cgaagccgcc cgacttgtac ccggcccgcg acgagccctt gtacaggccc
       61 tctgcgtcga ggtcgccgta gtcccgggca aagacgagca tgcgctcgcg atcctcggcg
      121 gacagcggtt gctcgaacgc attcttgtcg acggccttgt acagcagttc ggcgacgaat
      181 ccccgggtat ccgtggtgta gcgcccgacc cggaccggct tgccgccgaa atggtcttcc
      241 tcgtgagtgt aagcctggcg gctggcgttc gccttgacga tcagcggcac gtcgaggatc
      301 cggcaatagt gcagggtacg ccggtggtgg cccgggattc gcgccggccc ggcgttgaaa
      361 tagaggttgg gatcgtcgtc gaaatcgcag acctgccggt tgcccatctc gtcgattacc
      421 gtcccgtgcc gcaccgtgaa gttgcggccg cccgcgcgat gcgaagcctc caggaccgtg
      481 acccggtagc cggcgcgctc gagttcgtag gctatcgtca agcccgcgat gccggcgccg
      541 agaattgcga cgttctttcc gccgtccgcg acgtttttca tgtccagcgt tgcgaccggc
      601 cccgtgtctt cgagcagccc cagcgcccga ctggtctggt acacggctgt cgtgccgccg
      661 gcggcgccga cgaggttcag gaattgtcgt cgcgagagat tcttgtccat cggtgatgct
      721 gccgcttgtc tcttaacggg attggtacag ccgtgccgtg cttagttccc gaagacctcc
      781 gggaatagtt agctcattgt ttcgctgagc aggaaaagat ctgtcaggag ggcgcgcagg
      841 tgaatcgacg cgttcaggtt gtccaccagc tcggagctga tcgcctcctg cgcgaggacc
      901 agtgcgatgg ccgcccccga tttgcgggcg tcttccacga ccaccgctga aaacgggtcg
      961 ccgtccgccg ccagcgcggc gccgatcgac gcggccgctg ccgccatcga gttcagcgtg
     1021 tcgcggacct cggagccgcc cggcccctcg tcggtttccc ggccctgcgc cgcgtaggcg
     1081 aggaggaatt catagccggc ctcgatggcc tcgatactgt cctgcagtga ttcgctcacg
     1141 ttgtcatccg tgttgagtga tgaatgcccg ccgggatcga gtcaggaggc ttcgtccggg
     1201 gtccagcgct gcggatcgtg gtgcgccgcc agttcgtccc gggaaatcca gcccctgatc
     1261 atcgagcgcg tatagaagag tttctccggc cgcagcgcga agtagacgtg caggcagatg
     1321 agcccgatca gccctagggt cgatattccg tgaaggaaaa acacgatgcc cagcatcgcc
     1381 tcggacagcg cattgctgcg ctcccaccac ggcgtgtcga tcatggcgaa catgacgagt
     1441 ccgctgacga tgacgagcag cgtgagcacg ctgacggcca ggtgcatgct tttctgcgcc
     1501 gccgaatatt ttccgggctt gacgctgtcg tcgaagggtt ccttgaggtc gctgcccgaa
     1561 aaactcatct cgcgccagtc ctgggcgacg aacgcacgca cgatatgaaa gacgacggcg
     1621 atcgtgagaa ccaggccggc aatccagtga atctccagcc aggcgaactc gagcccgatg
     1681 atcgggaaca cgcccgtcac cagcaagacg aacacgctgg ccgccatgac ccagtgatag
     1741 gcgcggtcga tcgtgtcgtg ccgcgtgacg cgcgcgcctt cgctggacgg tttttcggcc
     1801 ctcttgcgaa gagcttcgat gactgcgtgc agggcaatga tgacgaacgc ggccacgatc
     1861 accagccaga gcaggtccca ggaaacgccc cggagaactt cctgccccca gacgtcgttc
     1921 ttgtatgagg caatatccac tcgatcaact ccgcatcgcg cgccgcacga ggttttccat
     1981 cagcggcagt ttgaaatgat tgtaattaag cggtttcgcg cccttgctgg cgagcggcgc
     2041 cactgaatcg gcaagttcgc cggacagcgg cttgccgcgc agcgcccgct cgacgctcct
     2101 gagccggtag ggcgtgcagg cgacgccgcc gcacacgatt cggctgtcgg caaccgtatc
     2161 gccatccatc gacatc
//
LOCUS       DATDAA010000410         2202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_273858,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000410 DATDAA010000000
VERSION     DATDAA010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273858"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VIS73_12230"
     CDS             <1..544
                     /locus_tag="VIS73_12230"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEY5763967.1"
                     /translation="TVNTERDSYAQIKFSDGGRVTLKPNTTVRLDQYRFAKEKPQEDS
                     FIYSLLRGGLRAVTGLLGRRSKDAYELKTATATVGIRGTTFSADDCATERTSPDCARL
                     APAVYVGVTDGEIVVRNAQGELGIAAGRFGLIERDKRPLFLSTDPGLHFTPPASFIAS
                     LAGGSTLNAGRAMECVVGQR"
     gene            683..>2202
                     /locus_tag="VIS73_12235"
     CDS             683..>2202
                     /locus_tag="VIS73_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY5763968.1"
                     /translation="MAAAQKSGTSPLPARLAGLLREARWLLVAVAAVYLFLILATFHQ
                     SDPGWSHSATGAIAQNAGGRVGAWLADLLLYLFGLSAYWWVGFCLAIVVSGYRRLHGA
                     PVPDHRSLGVMLAGFGLLLLASAGLEAIRLHSLQAELPLSPGGLVGDAVGAIAAGTLG
                     FTGATLALVALVAIGISLFSGMSWIKVAEFTGFVLESAYASAKSAWERRQDRKQGALA
                     REEREVVVESGRKREEDHPPLRIEPQLVEIKKSDRVQKERQVPLFDDLPDTPLPPIKL
                     LDEVRHDDEQVTADTLEFTSRLIEKKLADFGVTVKVLAAYPGPVITRYEIEPAVGVKG
                     SQIVNLIKDLARALSVVSIRVVETIPGKSCMGLEIPNPHRQAVRLSEILGSEAYHALH
                     APLALALGKDIAGNPVVVDLAKMPHLLVAGTTGSGKSVAINAMILSLLYKSEPRQVRL
                     ILVDPKMLELSVYQDIPHLLAPVVTDMKQAANALSWCVAEMDRRYKLMSWLGVRNLS"
BASE COUNT          344 a          744 c          741 g          373 t
ORIGIN      
        1 caccgtcaac acggagcgcg acagctacgc gcagatcaag ttctcggatg gggggcgcgt
       61 cacgctcaag ccgaacacta ccgtgcgtct tgaccagtac cgcttcgcga aggaaaagcc
      121 gcaggaagat tcgtttatct acagcctgct gcgtggaggc ctgcgggccg tgaccggact
      181 gctcgggcgg cgcagcaagg atgcctacga gctgaagacg gcgaccgcca ccgtcggcat
      241 acgcggaacg acgttctcgg cagacgactg cgccaccgag cggacctcgc ccgattgcgc
      301 gcgcctcgcg cccgccgtct acgtcggcgt cacggacggc gagatcgtgg tccgcaacgc
      361 gcagggcgag cttggcattg ccgccggtcg gttcggcctc atcgagcgcg acaagcgacc
      421 gctgttcctg tccaccgacc cgggcctgca tttcacgccg ccggcgagct tcatcgcctc
      481 cctggccggc ggcagcacac tgaatgcggg ccgggcgatg gaatgcgtcg tcggccagcg
      541 ctgagccgtc ggcagcgcag gccgtcccac ccgtcgtaag cccccgagtg ctgtgcgcgg
      601 tcatgcgcgt cgcatgaccg cgccgtggcc ggcgttgcgc cgcgacgggt acgatacaat
      661 cgtcgtttcc ggacccatcg cgatggcggc tgcacagaaa tccggcacct ctcccttgcc
      721 ggccagactt gcgggtctgc tacgcgaagc gcgctggctg ctggtcgcgg tcgcggcggt
      781 ttacctgttc ctcatcctcg cgacgtttca ccagtcggat cccggctggt cccatagcgc
      841 gaccggcgcc atcgcgcaaa acgcgggcgg ccgcgtcggt gcgtggctgg ccgacttgct
      901 gctctacctg ttcggcctgt ccgcctattg gtgggtcggg ttctgtctcg cgatcgtcgt
      961 ctccggctat cgccgcttgc acggcgcgcc ggtacccgac catcgctcgc tcggcgtgat
     1021 gctggcgggc ttcgggctgc tgctgctcgc gagcgcgggg ctcgaggcaa tccgcttgca
     1081 ttcactgcag gccgagctgc cgctctcacc cggcggactg gtcggcgacg cagtcggcgc
     1141 gatcgccgcg gggacgctgg gcttcaccgg cgccacgctg gcgctggtgg cgctggtggc
     1201 gatcggcatc agcctgttca gcggaatgtc ctggatcaag gtcgccgaat tcacgggctt
     1261 cgtgctcgag tcggcctacg cgtccgccaa gtccgcctgg gaacgcaggc aggatcgcaa
     1321 gcagggggcg ctggcgcgcg aggagcgcga ggtcgtcgtc gagagcggcc gcaagcgcga
     1381 ggaagaccat ccgccgttgc gcatcgagcc gcaactggtc gagatcaaga agtcggaccg
     1441 cgtgcagaag gagcgtcagg taccgctgtt cgacgacctg ccggacacgc cgctgccgcc
     1501 gatcaagctg ctcgacgaag ttcggcacga cgatgagcag gtcaccgccg acacgctgga
     1561 gttcacgtca cgcctcatcg aaaagaagct tgccgatttc ggcgtcacgg tgaaggtgct
     1621 cgcggcctac cccgggccgg tgatcacgcg ctacgagatc gagcctgcag tcggcgtgaa
     1681 gggcagccag atcgtcaatc tgatcaagga cctcgcgcgc gcattgtccg tggtgtcgat
     1741 tcgagtggtg gagaccatcc cgggcaagtc ctgcatgggg ctggagatcc caaatccgca
     1801 tcggcaggcc gtgcgcctgt ccgagatcct cggctcggag gcctaccacg cgctgcacgc
     1861 gccgctcgcc ctcgccctgg gcaaggacat cgccggcaac ccggtggtcg tcgatctcgc
     1921 caagatgccg catctgctgg tcgcgggcac gaccggttcc ggcaagtcgg tcgcgatcaa
     1981 cgcgatgatc ctttcgctgc tctacaagtc tgagccgcgc caggtgcgcc ttatcctggt
     2041 cgacccgaag atgctcgagt tgtcggtcta ccaggacatc ccgcacctgc tggcaccggt
     2101 ggtgaccgac atgaagcagg cggcgaacgc gctcagctgg tgcgtcgcgg agatggatcg
     2161 ccgctacaag ctgatgtcct ggctcggcgt gcgcaatctc tc
//
LOCUS       DATDAA010000411         4401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_484688,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000411 DATDAA010000000
VERSION     DATDAA010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484688"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1581)
                     /gene="pap"
                     /locus_tag="VIS73_12240"
     CDS             complement(94..1581)
                     /gene="pap"
                     /locus_tag="VIS73_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HEY5763969.1"
                     /translation="MFESAELGHTVDKKTFESEAARLRAALLEAQHQLVESAKFPVVV
                     LINGMDGAGRGETIHVLNEWMDPRFVQTHAFNIPTEEERERPFMWRFWRALPPKGRVG
                     ILFGSWYSELVHLMMGGGRDEEEEADLRAAAINRFERMLTDEGALVVKFWFHLSKQGQ
                     HDRLKELEGDPQTKWRVTKEDWKRYKEYSRFRQVAETILRNTSKANSPWYIIDGSDAR
                     FRSLTVAEILLEAIQQRLQRPGGSRRVAEAPIFHSALDRRNVLTELDLEQTLSKARYA
                     KELRAYQERLGQLMRSPAFASRAVVAVFEGNDAAGKGGAIRRITQAVDARHFQVVPIA
                     APTEEERAQPYLWRFWRHLPRTGRLTIFDRSWYGRVLVERVEGFAAEADWMRAYAEIN
                     DFEDQLIRHGIIVVKFWLAISPEEQLARFKEREAIEHKRFKITAEDWRNRDKWDAYET
                     AVCDMVDRTSTEIAPWTLVEANNKRFARVKVLKTLVKRLEAALKA"
     gene            complement(1623..2363)
                     /locus_tag="VIS73_12245"
     CDS             complement(1623..2363)
                     /locus_tag="VIS73_12245"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY5763970.1"
                     /translation="MRAMPERRIFVDVPIAPEQSMALPDSAAHHLARVLRVREGELLA
                     LFDGSGGEYPAVVAAVTRGGVQVQVGALRVVERESALQITLVQALQAADKMDTTIQKA
                     VELGVSRIVPVAAERSVLRLSGERAERRQAHWRHVVAAACEQCGRNRLPGVETIRTLP
                     EWFGEPSTVPLRLLLSPAGDAGLSALPPASAVELLVGPEGGFAEHELASAALAGYRPL
                     RAGPRVLRTETAGPALIAALQARWGDFA"
     gene            2464..3024
                     /locus_tag="VIS73_12250"
     CDS             2464..3024
                     /locus_tag="VIS73_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY5763971.1"
                     /translation="MVSLNPPACDFGWPAPAFKLPGTDGKQHDLASLAGPKGLLVMFI
                     CNHCPYVKAILDRLIRDCRELQALGIGVVAISANDTDAFPEDNFDNMKRLAQAKGFSF
                     PYLFDEDQSVARAYDAVCTPDFFGFNGKLELQYRGRFDASRKDTAPADARRDLFEAMK
                     QVAETGHGPTEQIPSIGCSIKWRGEG"
     gene            3030..3854
                     /locus_tag="VIS73_12255"
     CDS             3030..3854
                     /locus_tag="VIS73_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY5763972.1"
                     /translation="MTASAAELATALAPAQDALLALLREVADAEVMPRFLRVRSRQKD
                     DGTLFTEADLAAQHVLAEALPTIIDAPIVGEEMPEAEQRAAWAAGDGGLWTVDPIDGT
                     TNFINGLPFFALSVAYRLGGRTRLAATYNPITREMFHARDGGGAWLNDNRLPLRETAG
                     HLGRAVANIDYKRIPRDLADRLALHPPVYSVRNFGSSTLEWAYLAAGRLDVYLHGGQM
                     PWDYAAGELLLAEAGGRAGSLTDDTLAGPLWLRPVVAAASPALYREWANWLRNPAA"
     gene            3929..>4401
                     /locus_tag="VIS73_12260"
     CDS             3929..>4401
                     /locus_tag="VIS73_12260"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEY5763973.1"
                     /translation="MKRMTLSRYLIEAQREEGVVCADLRLLIEVVSRACKSIAIAVGK
                     GGLGEVLGEAGSDNVQGEAQKKLDVISNEILLDANEWGGHLAAMASEEMDLPHAIPNR
                     FPKGEYLLLFDPLDGSSNIDVNVSVGTIFSVLKCPEGVDPTSPEAFQQPGTKQVAA"
BASE COUNT          741 a         1568 c         1347 g          745 t
ORIGIN      
        1 gtagggcgcg acctggcgga accagtcaac gaagctcaac tcggcgccgc tgacctcggc
       61 ctcggccggt ttttccattt cactgtccat tctctaggcc ttcagtgccg cctcgaggcg
      121 cttcaccagc gtcttcagca ccttgacccg ggcaaaacgc ttgttgttgg cttcgaccag
      181 cgtccacggg gcgatctcgg tactggtgcg atcgaccata tcgcagaccg ctgtttcgta
      241 ggcgtcccat ttatctcggt tgcgccagtc ctcggcggtg atcttgaagc gcttgtgctc
      301 gattgcttcc cgctccttga atcgcgccag ctgctcttcg ggcgagatcg caagccagaa
      361 cttgacgacg atgatgccgt ggcggatcag ctggtcctcg aaatcgttga tctcggcgta
      421 ggcacgcatc cagtcggcct cggcggcgaa accctcgacc cgctcgacga gcacgcgtcc
      481 gtaccaggag cgatcgaaga tcgtcagcct gccggtgcgc ggcagatggc gccagaaacg
      541 ccacaggtag ggctgggcgc gctcctcctc ggtcggcgcg gcgatcggca cgacctggaa
      601 atgccgcgcg tcgacggcct gggtgatgcg ccggatggcg ccgcccttgc ccgccgcgtc
      661 gttgccctcg aaaaccgcga ccaccgcgcg cgaagcgaat gccggactgc gcatcaattg
      721 ccccagccgc tcctgatagg cgcgcagctc cttcgcgtag cgcgccttgc tcaacgtctg
      781 ctcgaggtcg agttcggtca gtacgttgcg ccggtcgagc gcgctgtgaa agattggcgc
      841 ttcggcaaca cgccgcgagc cgccaggccg ctgcagtcgc tgctggatcg cctcgagcag
      901 gatctcggca acggtcagcg aacggaagcg ggcgtcgctg ccgtcgatga tgtaccaggg
      961 cgagttggcc ttgctcgtgt tccgcaggat ggtctcggcg acctggcgaa agcggctgta
     1021 ttccttgtag cgtttccagt cttccttggt cactcgccac ttggtctgtg ggtcgccctc
     1081 gagttccttt agccgatcgt gctggccctg cttcgacagg tggaaccaga acttgacgac
     1141 cagcgcccct tcgtcggtga gcatccgctc gaagcggttg atcgctgcgg cacgcagatc
     1201 ggcctcttct tcttcgtcgc gcccgccacc catcatcagg tggacgagtt cgctgtacca
     1261 cgagccgaac aggatgccga ctcgaccctt ggggggcaat gcccgccaga agcgccacat
     1321 gaacggccgc tcgcgctcct cctcggtagg aatgttgaag gcgtgggtct gcacgaagcg
     1381 cgggtccatc cactcgttaa gcacgtggat cgtctcgccg cggccggcac cgtccatgcc
     1441 attgatcagc acgacgaccg gaaacttcgc gctctcgacc agctggtgct gcgcctcgag
     1501 cagcgccgcg cgcaagcgcg ccgcctcgct ctcgaaggtc ttcttatcga ccgtgtggcc
     1561 gagttccgcc gattcaaaca tgccgcatcc ccttagtccc taccgtcgtt tcatgttgcc
     1621 ggtcaggcga aatcccccca gcgcgcctgc agcgccgcga tgagtgccgg tccggcggtt
     1681 tcggtacgca gcacgcgcgg cccggcgcgc agaggccggt atccggccag cgccgcgctc
     1741 gccagttcat gctcggcaaa cccaccttcc gggccgacca gcaactcgac ggcgctcgcc
     1801 ggcggcaacg ccgacaggcc cgcgtcgccc gccggcgaga gtagcaggcg cagcgggacc
     1861 gtggaaggct cgccgaacca ctcgggcaag gtgcgaattg tctcgacgcc cggcagccga
     1921 ttgcggccgc actgctcgca ggccgccgcg accacatggc gccagtgcgc ctgtcgccgt
     1981 tcggcacgtt cgcccgaaag gcgcaagacg ctgcgttcgg ccgccactgg aacgatccgc
     2041 gagacgccca gctcaacggc cttctggatc gtcgtatcca tcttgtcggc tgcctgcagt
     2101 gcctgcacca gcgtgatctg cagggccgac tcgcgctcga cgacgcgcag cgcgccaacc
     2161 tgcacctgca cgccgccgcg cgtcacggcc gccacaactg ccggatactc gccgccgctg
     2221 ccatcaaaaa gtgccagcaa ttcgccctct cgcacgcgca gcacacgcgc cagatggtgg
     2281 gctgccgaat caggcagcgc catcgactgc tcgggtgcaa tcggcacatc aacaaagatc
     2341 cggcgttcgg gcattgctcg catgggcagg tggcctgccg aaatgaagtt gttaccattg
     2401 gccggattcg gccgggattc ccggcaccac cgccggagac ggcggcaccg gaaaggaggc
     2461 gctatggtat cgctcaaccc gcccgcttgc gattttggct ggcctgcacc ggcattcaag
     2521 ctgcccggaa ccgacggcaa gcagcatgac ctcgccagcc tcgccggccc caagggcctg
     2581 ctggtgatgt tcatctgcaa ccactgcccc tacgtcaagg ccatactcga ccggctaatc
     2641 cgcgactgcc gcgaactgca ggcactcggc atcggcgtcg tcgcgatcag cgccaacgat
     2701 accgacgcct tccccgaaga caacttcgac aacatgaagc ggctcgccca ggcgaagggc
     2761 ttttcgtttc cctatctctt cgacgaagac cagtcggttg cccgcgccta cgacgccgtg
     2821 tgcacccccg acttcttcgg tttcaacggt aagctcgaac ttcagtaccg cggccgattc
     2881 gacgcctcgc gcaaggacac cgcccccgcc gatgcgcggc gcgacctgtt cgaggcgatg
     2941 aagcaggttg ccgaaaccgg gcacggacca accgagcaga ttcccagcat cggctgctcg
     3001 ataaagtggc gcggcgaggg ctgagggcga tgaccgcttc cgccgctgaa ctcgctaccg
     3061 cactcgcgcc ggcccaagat gcgctgctcg ccttgctgcg agaagtcgcc gatgccgaag
     3121 tgatgccgcg cttcctgcgc gttcgcagcc ggcagaagga cgatggcaca ctgtttaccg
     3181 aagccgacct cgccgcccag cacgtactcg ccgaggcgct gccaacgatc atcgacgcgc
     3241 ccatcgtcgg cgaggaaatg ccagaagccg aacagcgtgc ggcgtgggct gccggcgacg
     3301 gcggcctgtg gaccgtcgac ccgatcgacg gcacgaccaa cttcatcaac ggcctgccct
     3361 tcttcgcgct gtcggtcgcc taccgtttag gcggccggac ccggctcgcc gccacctaca
     3421 acccgatcac gcgcgaaatg ttccatgccc gcgacggcgg cggcgcctgg ctcaacgaca
     3481 accgcttgcc cctgcgcgag accgccggcc atctaggccg tgccgtcgcc aacatcgact
     3541 acaagcgcat cccgcgggac ctcgccgacc gccttgcctt gcacccgccg gtctactcgg
     3601 tgcgcaactt cggttcgagc acgctcgaat gggcctacct cgcggccggc cgccttgatg
     3661 tctacctgca cggcgggcag atgccctggg attacgcggc cggtgaactg ctgctcgccg
     3721 aagccggcgg ccgcgccggc tcgctgaccg acgacacgct tgccgggccg ctctggctgc
     3781 gcccggtggt cgccgccgcc agcccggcgc tgtatcgcga atgggccaac tggcttcgca
     3841 acccggccgc ctgatcgacg cgtttaccga gaacgcgagc ggcgacccgt tagaattcgc
     3901 gccctccccc tcagctcagg acacgcccat gaaacgcatg acgctcagcc gctatctgat
     3961 cgaagcccag cgcgaggagg gcgtggtctg cgccgatctg cgcctgttga tcgaggtcgt
     4021 ctcgcgcgcc tgcaaatcaa ttgccatcgc cgtcggcaag ggcggactcg gcgaagtgct
     4081 cggcgaggcc ggcagcgaca acgtgcaggg cgaggcgcag aagaagctcg acgtcatctc
     4141 caacgagatc ctgctcgatg ccaacgagtg gggtggccac ctcgcggcga tggcctccga
     4201 ggaaatggac ctgccgcacg cgatccccaa ccgcttcccc aagggcgagt acctgctgct
     4261 cttcgatccg ctcgacggct cctcgaacat cgatgtcaac gtctcggtcg gaactatctt
     4321 ctcggtcctc aagtgccccg aaggcgtcga ccccacctca cccgaggcat tccagcagcc
     4381 gggcacgaaa caggttgccg c
//
LOCUS       DATDAA010000412         2836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_415106,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000412 DATDAA010000000
VERSION     DATDAA010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415106"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..886)
                     /locus_tag="VIS73_12265"
     CDS             complement(140..886)
                     /locus_tag="VIS73_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763974.1"
                     /translation="MTYRKLAGGILAASVAWLVVLAPAEPAIAQDGGGLIQVAEVQVK
                     MDRVGEFIDLQQQMAEAARAEGRSGTSVWQAAHGRMNMFHIVSGRDNFAAYDEGMDPP
                     MSGEEWARWVGRILQTIESRTVMTLRLYPDLVIPRGEDQERNLARLRITTVKPGMNDE
                     YEEWIRDKLMPALREGGQTGVSWQKVVTGGNPSTWFGVTEYSSFAQMDPPGPLDYMNE
                     RRRASMLDEAWSMTSGAKILVMRYRADLSY"
     gene            1089..1973
                     /locus_tag="VIS73_12270"
     CDS             1089..1973
                     /locus_tag="VIS73_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763975.1"
                     /translation="MPQSHAVSGWRFFWLAATMVSLAMIVAMARADLASAAAISSLIQ
                     LSVRCAVPWLYLAFAASSVQAVFPGAAGRWLLMNRKYIGLVFAAAMAWQGFFILWLVV
                     VHTDYYVEEVYVLRDAIEGVVGYLFLALMTITSFGFARRRLSARSWKLLHTAGIYYLW
                     GYAFIVYWWALFIYRNPVLLDYVYYWGGFAACCLRAAAWGKRQLAQSEKRARFHPSLS
                     YAGFALVGLGLLGGALGSLWQPLTDKYLYGYEITRIPELYLPYWPFEPFLPLAIIAAG
                     IYLVSRAGDQVTPEAQAV"
     gene            complement(1970..>2836)
                     /locus_tag="VIS73_12275"
     CDS             complement(1970..>2836)
                     /locus_tag="VIS73_12275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5763976.1"
                     /translation="RGLIEALIATVVVAAFAYWIEVTGVTAGVSLQRVIVIYSGIMLV
                     LSPILYFVMSDDEHEKPAEKEDYNAQQLWRDVGTIGRKPEIWLCTICILIGYQLFWAT
                     YSFSAYIQSELGMSAVAAGSITAAKLWMRPIGAATAGFAGDLIDRERVLAGLYIGSAA
                     ALAVITMLPVGAGFAAAGGIVLAIGVLTYGIRGIFWATLESAGVEARIKGLAIGMICL
                     VGFSPDIYLPLINGYLLERYPGRTGYAIYFHGIAAMGLIGAWAALKLRSMAQAKAGLS
                     VAASEQPVVETG"
BASE COUNT          474 a          897 c          919 g          546 t
ORIGIN      
        1 ccgctatcga agcgcagcgg tcgaaatgat cgcgcctgcc cggattcggc ggcggcggga
       61 tggtcgcggg gtggccggtc cgaaaaggcc tggatgccgg gaacgcgccc cgaccggggg
      121 ccggggcgcg aacggtgagt cagtaactaa ggtcggcgcg atagcgcatg accaggatct
      181 tggcgcctga agtcatcgac cacgcctcgt ccagcatcga ggcccggcga cgctcgttca
      241 tgtagtccag cggacccggc gggtccattt gggcgaaact ggaatactcc gtaacgccga
      301 accaggtaga ggggttgccc ccggtgacga ccttttgcca cgaaacgccg gtctggccgc
      361 cctcccgaag agccggcatc agcttgtcgc gaatccattc ctcatactcg tcattcatgc
      421 ccggttttac ggtggtaatc cgcaacctgg ccaggttccg ttcctggtcc tcgccgcgag
      481 gaataaccag gtcgggatag aggcgaagcg tcatcacggt ccgcgattcg atcgtctgca
      541 gaatgcggcc gacccatcgc gcccattctt cgcccgacat cggcgggtcc atgccttcgt
      601 cataggcggc aaagttgtcc ctgccggaaa cgatgtggaa catgttcatg cggccgtgag
      661 cggcttgcca cacgctggtg ccgctgcggc cttccgccct tgccgcctcg gccatttgct
      721 gctgcaggtc gatgaattca ccgacgcgat ccatcttgac ctggacctcg gccacctgga
      781 taagaccccc accgtcctgg gcaatcgcgg gttctgcggg cgcgaggacg accagccagg
      841 caacggacgc ggcgagaatg ccgcctgcga gttttctgta agtcattttc ttctccttgt
      901 tttcgtgtcc ggcaaaatgg gactgatcgg agcccggacg ttcctgacag tgtagtggag
      961 aatgcgggct ccgccagcgg aacgggtgac gggcgtgctc gcccctgtcc cggcctcagc
     1021 ggcgccgggg tttccaggcc ccggattatt gctaccatgg cctgtcgccg ggtaaacact
     1081 ggaagtcgat gccacagagt cacgcagtca gcggatggcg gtttttctgg ctggccgcca
     1141 cgatggtctc cctcgcgatg atcgttgcga tggcgcgcgc cgatcttgcg agcgccgccg
     1201 ccatatcgtc gctgatccag ctctccgtgc gctgcgcggt cccctggctt tatctcgcgt
     1261 tcgcggcgtc gtccgtgcag gccgtgttcc ccggggccgc cggcaggtgg cttttgatga
     1321 acaggaagta catcggcctg gtgtttgcgg cggccatggc gtggcaggga ttcttcatcc
     1381 tgtggctggt cgttgtgcac acggattact acgtcgaaga ggtgtacgtg cttcgcgacg
     1441 cgatagaagg cgtggtcggc taccttttcc ttgcgctcat gacgatcacc tcgttcgggt
     1501 tcgcgcgccg gcggctgtcg gccaggtcct ggaaactgct gcacaccgcc ggcatctatt
     1561 acctctgggg ctacgcattc atcgtttact ggtgggcgct ctttatctac cggaacccgg
     1621 ttcttctcga ttacgtttat tactggggcg gattcgcggc ctgttgcctg cgggccgcgg
     1681 cctggggaaa gcggcagctt gcgcaaagcg aaaaaagggc gcggtttcac ccgtcgctca
     1741 gctatgcggg cttcgcgctc gtcgggctgg gtttgctggg aggagctctc ggttccctgt
     1801 ggcagccgct caccgacaag tacctgtacg gatacgagat tacgcggatc ccggaactct
     1861 acctgccgta ctggcctttc gagcctttcc tgccgctggc catcattgcg gcgggcatct
     1921 acctggtctc cagggcgggc gaccaggtta cgccggaggc gcaggcggtc tagcctgttt
     1981 cgacgacggg ctgttcgctc gcggcgaccg aaagtcccgc tttcgcctgc gccatcgaac
     2041 gcagcttgag cgccgcccag gcgccgatca gccccatcgc ggcaatgccg tggaagtaaa
     2101 tggcgtaacc cgtgcgcccg ggataccgct ccagcaggta gccgttgatc agcggcaggt
     2161 agatgtcggg cgagaagccg acgaggcaga tcatgccgat cgcgagcccc ttgatgcggg
     2221 cctcgacgcc cgccgattcg agcgtcgccc agaagatgcc gcggatgccg tatgtcaaaa
     2281 cgccgatggc cagcacgatg ccgccggcgg cggcgaaacc cgcgccgacc ggcagcatgg
     2341 tgatgacggc gagcgcggcg gcgctgccga tgtaaaggcc ggcgagcacc cgttcgcggt
     2401 cgatgaggtc gccggcaaaa cccgccgtgg ccgcgccgat ggggcgcatc cacagtttcg
     2461 ccgcggtgat cgagccggcg gccacggcgc tcatgccgag ttcgctctgg atataggcgg
     2521 agaacgaata ggttgcccag aaaagctggt agccgatcaa tatgcagatc gtgcagagcc
     2581 atatctccgg tttgcgcccg atcgtgccga cgtcccgcca cagctgctgc gcgttatagt
     2641 cctccttctc ggccggcttt tcgtgctcgt cgtcgctcat gacgaaatac aggatcggcg
     2701 aaagcacgag catgatgccg gaataaatga cgatgacccg ctgcagcgac acgcccgcag
     2761 tcacgccggt gacttcgatc cagtaggcga aagccgcgac gacgacggtc gcgatcagcg
     2821 cctcgatcag gccgcg
//
LOCUS       DATDAA010000413         5437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_274127,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000413 DATDAA010000000
VERSION     DATDAA010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274127"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /gene="rimO"
                     /locus_tag="VIS73_12280"
     CDS             complement(<1..1058)
                     /gene="rimO"
                     /locus_tag="VIS73_12280"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY5763977.1"
                     /translation="MTQRGSKADAGKPPRVGFVSLGCPKALVDSENILTQLRAEGYVV
                     APDYAGADLVVVNTCGFIDAAVEESLAAIGEAVEENGRVIVTGCLGARDEGELVRRAH
                     PKVMAVTGPHAAAEVMQHIHAALPPRHDPLTSLVPSAGVKLTPGHYAYLKISEGCNHG
                     CTFCIIPSLRGPLVSRPIGDVIEEAQRLAESGVRELLLIAQDLSAYGVDVKYRTGFHG
                     GRPVRTRLKELCEELAKLGIWVRLHYVYPYPAVDDLIPLMAEGKILPYLDVPLQHASQ
                     RILKAMKRPASAENALARIRAWRAICPDITIRSTFIAGFPGETEADFEELLAFLEEAE
                     LDRVGCFAYSDVDGATANA"
     gene            complement(1055..1615)
                     /gene="phaR"
                     /locus_tag="VIS73_12285"
     CDS             complement(1055..1615)
                     /gene="phaR"
                     /locus_tag="VIS73_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEY5763978.1"
                     /translation="MSDQARTIKKYPNRRLYDTHTSSYITLADVKDLVMSFEEFKVID
                     AKSGDDLTRSILLQIILEEETANVPLFSTELLAQMIRFYGHAMQGMLGKYLENNIRTF
                     VDFQAKMQPQSRPLSAGDAQMQNELWAQFLNFQGPAMQTMMSSYMEQSRKMVEQLQEQ
                     MESQTRNMFTGFNYPAGSEPKNTGKE"
     gene            complement(1681..2424)
                     /gene="phbB"
                     /locus_tag="VIS73_12290"
     CDS             complement(1681..2424)
                     /gene="phbB"
                     /locus_tag="VIS73_12290"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEY5763979.1"
                     /translation="MTQRVAFVTGAMGGIGTAICQELAKAGHKVVAAYHPEFDKPDEW
                     MAEQQAAGFKDFITVAGDVGDEASCAKMLAEAETKAGQIDILVNNAGITRDRFFVKMD
                     RGQWDAVINTNLNSAYNLTKPVLAKMTERGWGRIINISSVNGVKGQAGQANYSAAKAG
                     LIGFTKAVAGETAAKGVTVNAIAPGYIATKMVMAIREDILKGIVDSVPMKRLGKPEEI
                     GAACVYLASENAGFITGATLNINGGLYCQ"
     gene            complement(2468..4234)
                     /gene="phaC"
                     /locus_tag="VIS73_12295"
     CDS             complement(2468..4234)
                     /gene="phaC"
                     /locus_tag="VIS73_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEY5763980.1"
                     /translation="MFNVQDGIFPAAQNFAQSGQALADQFLKLVGQAMSAQQGKPVAL
                     PSPDPAELGDLQQRFVSDQARLWQSMLQKGAEQIPGKGAAVPDAKGAAEFRVSPEPGD
                     RRFAAKEWRESPVFDYIQQAYLLNARYLREFVELVPVEEGRLKDRVRFIARQYIDALA
                     PSNFAGTNPEFIRLAVESKGESVRSGVENLIGDFEKGRISMTDESVFEVGKNLATTPG
                     SVIFENELIQLIQYAPTTEKVAKRPFLIVPPCINKYYILDLQPENSFVRFAVEQGHTV
                     FLVSWRNPKEAQAKRTWDDYLEQGPITAIGVVREITKAKQINALGFCVGGTILTSALA
                     VLKARGEDPVASLTLLTTLLDFSDTGEIGLFIDEQSVAAREATIGQGGLLTARELSGT
                     FSALRANDLVWNYVANNYLKGQKPQAFDLLYWNGDNTNLPGPFACWYMRNLYHANNLR
                     VPGKLEMCGVKVDLGKLEMPAYLLATREDHIVPWQTAYQSTRLLGGKTRFVLGASGHI
                     AGVINPAAKNKRSHWLEGNQRSGAEDWLSSAKEVPGSWWTDWAEWLGSHGDGEVAAPK
                     TPGNKTYKVIEPAPGRYVREKA"
     gene            4440..4628
                     /locus_tag="VIS73_12300"
     CDS             4440..4628
                     /locus_tag="VIS73_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763981.1"
                     /translation="MYVVAIGWLYVVTLMAFAEPSIVAGVLTFIFYGLLPVSLLFWLT
                     GAFARRSARRRDKGARGT"
     gene            complement(4691..5218)
                     /locus_tag="VIS73_12305"
     CDS             complement(4691..5218)
                     /locus_tag="VIS73_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763982.1"
                     /translation="MVDLLFDYLRDLEANRCDPKGQSGKISVGVVEVGANCEEVSLTA
                     STPGWLGLFVKVGYEQSLRYEYVKAGKERFLAQQAGLDGHVRPHFGEYGAAFDGKLTI
                     YAGGFAKAEAAGAGIDAKAGGSFTVDGNGNVSDFARQTEVSASGSVGGKGVSGGGERT
                     IYKERLAFSPSRSEN"
BASE COUNT          971 a         1717 c         1646 g         1103 t
ORIGIN      
        1 gcgttggccg ttgcgccatc gacatcggag taggcaaaac agccgacgcg atccagctcg
       61 gcctcctcga ggaaggccag cagctcctcg aagtcggcct cggtctcgcc cggaaagccg
      121 gcaataaagg tgctgcggat cgtgatgtcg ggacagatcg cccgccaagc gcgaatccgc
      181 gccagcgcgt tctcggccga tgcaggccgc ttcatggcct tgaggatccg ctggctggcg
      241 tgctgcagcg gtacgtcgag gtagggcagg atcttgcctt cggccatcaa cggaatcagg
      301 tcgtcgacgg ccggataggg atagacgtaa tgcaggcgaa cccagatgcc gagcttggcg
      361 agttcctcgc acaactcctt gaggcgcgtg cgcaccggac gcccgccgtg aaacccggtc
      421 cggtacttga cgtcgacacc gtaggcggag aggtcctgcg cgatcagcag cagttcacgc
      481 acgcccgatt cggccagccg ttgtgcttct tcgatcacgt cgccgatcgg ccgcgagacg
      541 agcggcccac gcagcgaggg aatgatgcag aaggtgcagc cgtggttgca gccttcggaa
      601 atcttcagat aggcatagtg gcccggcgtc agcttgacgc ccgccgacgg caccaggctc
      661 gttaaaggat cgtggcgcgg cggcagtgct gcgtggatgt gctgcatgac ctccgccgcc
      721 gcatgcggcc cggtcaccgc catcactttg ggatgagccc gacgaaccag ttcaccctcg
      781 tcgcgtgcgc caaggcagcc ggtgacgatg acccgaccgt tctcctcaac cgcctcgccg
      841 atcgccgcca acgactcctc gaccgcggca tcgatgaagc cgcaggtgtt gacgacgacg
      901 agatcggccc ccgcgtagtc gggcgcgacg acgtagccct cggcgcgcaa ctgggtgagg
      961 atgttttccg aatcgaccag cgctttcgga caacccagag acacgaaacc gacgcgcggc
     1021 ggcttgccgg cgtcggcctt cgaaccgcgt tgtgtcattc cttgcccgtg ttctttggct
     1081 cggagccggc cgggtagttg aatccggtaa acatgttgcg cgtctggctt tccatctgtt
     1141 cctgcaattg ctcgaccatc ttgcggctct gctccatgta ggacgacatc atcgtctgca
     1201 tcgccggacc ctggaagttc aggaactgcg cccagagttc gttctgcatc tgggcatcgc
     1261 ctgcgctgag cggccgcgac tgcggctgca tcttggcctg gaaatcgacg aaggtacgga
     1321 tgttgttctc cagatacttg ccgagcattc cctgcatcgc gtgcccgtag aagcggatca
     1381 tctgcgcgag cagctcggtc gagaacaacg gcacgtttgc cgtttcttct tcgagaatga
     1441 tctgcaacag aatgctgcgt gtcaggtcgt cgcccgactt cgcatcgatt accttgaatt
     1501 cctcaaagct cattaccagg tccttgacat ccgccagggt gatgtagctg ctggtatggg
     1561 tgtcgtacag cctccgattc ggatacttct tgatcgttcg cgcctggtct gacacttgtt
     1621 cctccctcat tcgcgccccg ctcgggcggc cagcagctgc cgaccgccct cccgcgtcgc
     1681 ttactggcag tacagcccgc cgttaatgtt tagcgtcgcg ccggtgatga aaccggcatt
     1741 ctctgatgcc agataaacgc aggccgcacc gatttcttcg ggcttgccca gacgcttcat
     1801 cggtaccgaa tcaacgatgc ccttgaggat gtcctcgcgg atcgccatga ccatcttggt
     1861 ggcgatgtag cccggcgcaa tcgcgttcac agtgacgcct ttggcggcgg tttcgccggc
     1921 cactgccttg gtgaagccga tcaggccggc cttcgctgcc gagtagttgg cctggcctgc
     1981 ctggcccttg acgccgttga ccgacgagat gttgatgatg cggccccagc cacgctcggt
     2041 catcttggcg agaaccggct tggtcaggtt gtaggcgctg ttgaggttgg tgttgatcac
     2101 cgcatcccac tggccacgat ccatcttgac gaagaaacgg tcacgcgtga taccggcatt
     2161 gttcaccaga atgtcgatct gcccagcctt cgtctcagct tcggcaagca tcttcgcgca
     2221 cgaggcctcg tcaccgacgt caccggcaac cgtaatgaaa tccttgaaac cggcagcctg
     2281 ctgctcggcc atccactcgt ccggcttgtc gaattccggg tgatacgcgg cgacgacctt
     2341 gtgaccggcc ttggcgagtt cctggcaaat tgcagttccg atgccaccca tggcaccggt
     2401 gacgaaagcg acgcgttgag tcatctgttc ttctcctgtt gttgcgggtt gtgaaatcgt
     2461 aagtcgatca ggctttttcg cggacgtagc gtccgggagc tggctcgatc accttgtagg
     2521 tcttgttgcc cggcgtcttg ggcgcggcca cttcgccatc gccgtggctg ccgagccatt
     2581 cggcccagtc ggtccaccaa ctgccgggca cttctttggc gctcgaaagc cagtcctcgg
     2641 cgccactgcg ctggttgccc tcgagccagt gactgcgctt gttctttgcg gcgggattga
     2701 tgacgccggc gatatggccg ctcgccccga gcacgaatcg ggtcttgccg ccaagcaatc
     2761 gcgtcgactg gtaggccgtc tgccagggaa cgatgtgatc ctcccgggtt gccagcaggt
     2821 aggccggcat ctccaacttg ccgaggtcta ccttgacgcc gcacatctcg agcttgcccg
     2881 gcacgcgcag gttgttggcg tggtacaggt tacgcatgta ccagcaggca aagggacccg
     2941 gcagattggt gttgtcgccg ttccagtaga gcagatcgaa ggcctgcggc ttctggccct
     3001 tgagatagtt gttggcgacg taattccaga ccagatcgtt ggcgcgcagc gccgaaaagg
     3061 tcccggacaa ttcgcgcgcg gtgagcaggc caccctgacc gatcgtcgcc tcgcgcgcgg
     3121 caacgctctg ctcgtcgatg aacaggccga tctcgccggt atcggaaaaa tcgagcagcg
     3181 tggtcagcag cgtcaggctc gcgaccgggt cctcgccgcg cgccttgagc accgccagcg
     3241 ccgaggtcag gatggtgccg ccgacgcaaa agcccagcgc attgatctgt ttcgccttgg
     3301 tgatctcgcg aacgacgccg atcgcggtga tcggcccctg ctcgaggtaa tcgtcccagg
     3361 tgcgcttggc ctgcgcctcc ttgggattgc gccaggaaac cagaaagacc gtatgcccct
     3421 gttccactgc gaagcggaca aaggagttct cgggctgcag gtcgaggatg tagtacttgt
     3481 tgatgcacgg cgggacgatc aggaacggcc gcttggcgac cttctcggtc gtcggcgcat
     3541 actggatcag ctggatcagt tcgttctcga agatcacgct gcccggcgtc gtcgcgaggt
     3601 tcttgccgac ctcaaagacc gactcgtcgg tcatcgagat gcgtcccttc tcgaaatcgc
     3661 cgatcaggtt ttcaacaccg ctgcgaacgc tctcgccctt cgactccacg gccaagcgaa
     3721 tgaactcggg attggttccg gcgaagttcg acggtgcgag cgcatcgatg tactggcggg
     3781 cgataaagcg cacgcgatcc ttgaggcgcc cttcttcgac cgggaccagc tcgacgaact
     3841 cgcgaaggta ccgggcgttt agcaggtaag cctgctggat gtaatcgaag accgggcttt
     3901 cacgccattc cttggcagcg aagcggcggt cgcccggctc ggggctgacg cggaactccg
     3961 cggctccctt ggcatcggga actgccgcgc ctttgcccgg tatttgctcg gcaccctttt
     4021 gcagcatgct ttgccagagg cgtgcctgat cagaaacaaa acgttgttgg aggtcgccaa
     4081 gctctgcagg atcgggcgat ggcaaggcca ccggcttgcc ctgctgggcg gacatcgcct
     4141 gcccgactag cttgaggaac tggtcggcca aagcctgccc ggactgggcg aaattctgcg
     4201 ctgcgggaaa aatgccgtcc tgcacgttaa acattcgttc gtgcctttcg gaagtcgctt
     4261 gcgggctgag aagtggcacc agataagggc gccaccgatt cttgggaggg tttcattctg
     4321 catggcagca tcgtcgagtg tcaataagac atgcggcggc gcgcaacgtt cgagaatgac
     4381 catatatcag tcgatgttat cgtccgcggc agaacttgtt ttccgaggag aagcgaagga
     4441 tgtacgtcgt cgcaatcggc tggctctacg tcgtcacgct gatggccttt gccgagccca
     4501 gtatcgtcgc tggcgtgctg accttcatct tctacggact cctgccggta tcgctgctgt
     4561 tttggctcac tggagcattc gcccgccgat ccgcccggcg cagagataaa ggcgcacgcg
     4621 gaacctaaac ggcagcagcc aacgtgtagc tgtctcagaa gaaacgacta caagggctcg
     4681 cacgcccaat ctagttttcg gagcggctcg ggctgaacgc caggcgctcc ttgtagatcg
     4741 tcctctcgcc gccgccgctg acccccttgc cgccgaccga acccgaggcg cttacctcgg
     4801 tctgtcgggc gaaatcgctg acgttgccgt tgccgtcgac ggtaaaagag ccgccggcct
     4861 tggcatcgat tccggcgccg gccgcctcgg ccttggcgaa accgccggcg tagatggtca
     4921 gcttgccgtc gaaggccgcc ccgtactcgc cgaaatgcgg cctgacatgg ccgtcgagtc
     4981 cggcctgctg ggcgaggaaa cgctccttgc cggccttcac gtattcgtaa cgcaacgact
     5041 gctcatagcc gaccttgacg aacaggccca gccagcccgg cgtcgaggcg gtgagcgaaa
     5101 cctcctcgca gttcgcaccg acctcgacga caccgaccga aatctttccc gactgtccct
     5161 tcggatcgca gcggttcgcc tcgaggtccc tcaaatagtc aaacaaaagg tcaaccatgc
     5221 gtttgttcga agctctgcat gcatcggcgc cttgtgcctg cgccgaaacg aacctgccga
     5281 cttggcccgc cagcgacagc gtcgcttctg cagcgaagcg cttcagtcgc gcgttccctt
     5341 cctggtgatg cgccggatcg ccgaaatacg aaacaacagc cgacagtcgc tttgatgcat
     5401 ccgtaaacag atctcgaaac gccgcgtcga aagtttt
//
LOCUS       DATDAA010000414         3861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_274184,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000414 DATDAA010000000
VERSION     DATDAA010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274184"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..478)
                     /gene="ccoS"
                     /locus_tag="VIS73_12310"
     CDS             complement(263..478)
                     /gene="ccoS"
                     /locus_tag="VIS73_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEY5763983.1"
                     /translation="MEILYLLIPISLVLVFVIGAAFFWSVRGGQYDDLEGPAHRILLD
                     DDRPRSVRERETDKDEKQSDDRKSELP"
     gene            complement(478..3009)
                     /locus_tag="VIS73_12315"
     CDS             complement(478..3009)
                     /locus_tag="VIS73_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY5763984.1"
                     /translation="MKHDPSGDCYHCGLPVPASVELSVTLDGAQQPMCCAGCQAVASA
                     IIDNGLADYYRHRDALPNSPREAIPAALDEMRLFDHAAVQKTLVRDLGDDAREVSLML
                     EGITCAACVWLNEQHLARVPGVTAVSINYATRRARVQWDSRRCALSDILAAVAAIGYR
                     AHPYDVSRHEAIAGRERREAIWRLFVAGFGAMQVMMYAIPTYVAADGEMTEDIRQLMH
                     WAGLILTLPVMAYSARPFFTNALRDLRLRRLGMDVPVALGLGVAFAASVWATVSAHGA
                     VYFDSVSMFVFLLLGGRFLEMRARQKAVAIGESIARLVPALASRIPGWPATREPQTVA
                     AAELVAGDFVLVKAGETVAADGVLVEGVSSFDESLLTGESLPRARAVGESLTAGTCNV
                     DSPVVFRVEQGGDETRLATIVRLMERAASERPAIVALADRVASWFVFTMLCIAALVFY
                     YWFGVDPERAIWVTVSVLVATCPCALSLATPVALTVANGTLAQQGLVVSRAGSVEALA
                     QATRIVFDKTGTLTTGRPGLERVDVLAGDDDKTCLTLAARLEAASAHPLARALRDAAT
                     RHGAAGGQMAENILVAPGQGLEGSVDGRRLRIGRPDYVAGLHGRPLTPAMSDAAAGAA
                     SLVALGDTQGWIALFVLRDELRPGAANAIAALRKMGYRLAILSGDHASAVEAVAGSLA
                     IREWHAGKLPAEKLERVRRWQSDGERVLMIGDGINDGPVLAQAQVSIAMGAGAQLAKV
                     QADCILIGDDLDSLVGCLRTARRARRVIAQNLAWALAYNALVIPFAALGWVTPWMAGI
                     GMSGSSLVVVANALRIARPLRGRSVRGLDSANGTSSVLTAATPEA"
     gene            complement(3126..>3861)
                     /locus_tag="VIS73_12320"
     CDS             complement(3126..>3861)
                     /locus_tag="VIS73_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEY5763985.1"
                     /translation="GGGSGLGAATARMAVAAGGKAVIADVNAAAGEVLAKELGGDCRF
                     VHCDVADEVSAGSAVDFAIKEYGSLEVLVNCAGVAPAEKTLGKEGPHRLDSFARTIQI
                     NLIGTFNMIRLAAEAMSRNQPDADGCRGVIVNTASVAAFDGQMGQAAYAASKGGIVGM
                     TLPIARDLARNGIRVMTIAPGIMETPMLAGMPPEVQQALGSMVPFPSRLGRPQEFAAL
                     FRHIVENNYLNGEVIRMDGAIRMQPK"
BASE COUNT          733 a         1351 c         1190 g          587 t
ORIGIN      
        1 ttcgtgaatc ggcaaaaaaa ccttgtctgg cgcaaggtca ttgccgcgaa ccaccgaaat
       61 cagcctctca agcggacaca tgcggcatcg atctacctat taacaatgcc aacgccattg
      121 cggcgtagca aaaaaaacgg gcgcatcttg cgacgcgccc caaccccaac agagaaacat
      181 ccagttttcc ggtgcgcacc ctgcaccgcg ccggagaccg cggcaattat cccctggcgg
      241 ccggatgtcc cattgaccct agtcaaggaa gctcgctttt cctgtcgtcg ctttgttttt
      301 cgtccttatc ggtctcgcgc tcgcggaccg atcgcggacg gtcgtcatcg agcaggatcc
      361 ggtgcgcggg cccttcgagg tcgtcgtatt ggccaccgcg caccgaccag aagaatgccg
      421 cgccgatcac gaataccagc accagcgaga tcggaatcag caggtaaagg atctccatca
      481 agcctccggc gtcgctgctg tcagcaccga cgatgtgcca ttcgcagaat ccagtccacg
      541 taccgacctg ccgcgcaggg gcctcgcaat gcgcaaggcg ttggcgacaa cgaccagcga
      601 acttccggac atgccgattc cggccatcca cggcgtcacc cagccgagcg ccgcgaaggg
      661 aatgacgagc gcgttgtaag ccaacgccca ggcaaggttc tgcgcaatga cccggcgcgc
      721 gcgacgcgcc gtgcgcaggc agccaaccag gctgtcgagg tcatcaccga tcaggatgca
      781 gtcggcctgg accttggcga gttgcgcgcc ggcgcccatc gcgatcgaaa cctgcgcctg
      841 cgccagcacc ggtccgtcgt tgatcccgtc gccgatcatc aagacccgct cgccgtcgct
      901 ttgccagcgg cgaacccgct cgagtttctc ggcaggcagc ttgccggcgt gccattcgcg
      961 gatcgccagc gatccggcga cggcctcgac cgccgacgca tggtcgccgc tgaggatggc
     1021 cagccggtag cccatcttgc ggagtgctgc gatggcattc gccgcaccgg gacgcagctc
     1081 gtcgcgcaga acgaacagcg cgatccagcc ctgcgtatcg ccgagcgcca ccagcgatgc
     1141 ggcgccggca gcagcatcgc tcatcgccgg cgtcagaggc cgcccatgca ggccggcaac
     1201 gtaatcgggg cgaccgatgc gcagccgccg gccatcgacc gagccttcca gcccctgccc
     1261 cggcgcgacg aggatgtttt cggccatctg tccgccggca gcaccgtgcc gcgtggccgc
     1321 gtcacgcagc gcacgtgcca gcggatgtgc cgacgccgct tcgagccgcg ccgcaagcgt
     1381 caggcaggtc ttgtcgtcgt caccggccag tacgtcgacg cgctcgagtc cagggcgtcc
     1441 ggtagtcaag gtaccggtct tgtcgaagac gatacgggtc gcctgcgcca gcgcctctac
     1501 tgacccggcg cgcgaaacca ccaagccttg ttgggcgagc gtgccgttgg caacggtcag
     1561 cgccaccggc gtggccagcg acagcgcgca ggggcaggtc gccaccagca ccgaaacggt
     1621 gacccagatt gcgcgctcgg ggtcgacgcc aaaccagtag taaaagacta gcgcagcaat
     1681 acagagcatg gtgaagacga accacgaggc cacccgatcg gccagcgcga cgatcgccgg
     1741 ccgctcggag gcagcacgct ccatcaggcg gacgatggtc gccaggcggg tttcgtcgcc
     1801 gccctgctcg acgcgaaaga ccactggcga gtcgacgttg caggtgccgg cggtcagcga
     1861 ctcgccgacg gcgcgagcac gcggcagact ctcgccggtg agcaaggact cgtcgaagct
     1921 gctgacgcct tccaccagaa ccccgtcggc ggcaacggtt tcgccggcct tgaccagcac
     1981 gaaatcgccg gcgacaagtt cagcagccgc aaccgtctgc ggctcgcgcg tcgccggcca
     2041 gccgggaatg cgactggcca acgccggcac cagtcgggca attgactcgc cgatggcaac
     2101 cgccttctgc cgcgcccgca tttcgaggaa acgcccgccg aggagcagga agacgaacat
     2161 gcttaccgag tcgaaataga ctgcgccgtg agcgctgact gtcgcccaga cgctcgccgc
     2221 aaaagcgacg ccaagcccca acgcaaccgg cacatccatg ccgaggcggc gcagcctcag
     2281 gtcgcgcagt gcattggtga agaaaggccg cgccgagtac gccatcacag gcagggtcaa
     2341 gatcagcccg gcccagtgca tcaattggcg gatgtcctcg gtcatctcgc cgtcggcggc
     2401 aacgtaggtc gggatcgcgt acatcatcac ctgcatggcg ccgaaaccgg caacgaacag
     2461 gcgccagatc gcctcgcgcc gctcgcggcc ggcgatcgcc tcgtggcgcg agacgtcgta
     2521 gggatgtgcg cgatagccaa tcgccgcgac ggctgcgagg atgtcggaga gtgcgcagcg
     2581 gcggctgtcc cactggacgc gcgcgcgccg cgtcgcgtag ttgatgctga cggcggttac
     2641 gcccggcacc cgcgccaggt gctgttcgtt gagccagaca caggcggcgc aggtaatgcc
     2701 ttcaagcatc agcgagactt cgcgggcatc atcgccgaga tcgcgaacca gggtcttctg
     2761 caccgccgcg tggtcgaaca gccgcatctc atcgagcgcc gccggaatcg cctcgcgcgg
     2821 cgagttcggt agcgcgtcgc gatgccggta ataatcggcc aaaccgttat cgatgatcgc
     2881 cgaggcgacg gcctggcaac cggcgcagca catcggctgc tgcgcaccgt cgagagttac
     2941 cgacagctcg acgctggcgg gaaccggcag gccgcagtgg taacaatcgc cgctgggatc
     3001 gtgcttcaag atcggggcca accgcggcgt ctcgccgccg gccaggagga tttcagagcc
     3061 gccagtatac aagggccgca gtcgtcgccc gaccgtgcca gaccgccacc cgaacggcca
     3121 atctgctact tcggctgcat gcggatcgcg ccgtccatgc ggattacctc gccgttgagg
     3181 tagttgttct caacgatgtg ccgaaacagc gccgcgaact cctgcggccg gccaaggcgc
     3241 gatggaaacg gcaccatcga ccccagcgcc tgctgcacct cgggcggcat cccggccagc
     3301 atcggcgttt ccatgatccc cggggcaatc gtcataaccc ggatgccatt gcgcgccaga
     3361 tcgcgggcaa tcggcagagt catgccgaca atgccgccct tcgacgccgc gtacgctgcc
     3421 tggcccatct ggccatcgaa ggccgcaacc gaggccgtat tgacaatcac gccccggcag
     3481 ccatcggcat cgggctggtt acggctcatc gcctcggccg ccagccggat catgttgaag
     3541 gtgccgatca gatttatctg aatcgtccgt gcgaaggaat caagccggtg cggcccttcc
     3601 ttgccgagcg ttttctcggc cggcgcgacg cctgcgcagt taactaggac ctcaagcgac
     3661 ccatattcct taatcgcgaa atcgactgcg ctaccagcgc tcacctcgtc tgcgacgtcg
     3721 cagtgaacaa agcgacaatc accacctagc tctttggcaa gcacctctcc ggctgccgca
     3781 ttcacatcag caatcaccgc ttttccgccc gctgcgacgg ccattcgcgc cgttgccgca
     3841 cccaagcctg aaccgccccc g
//
LOCUS       DATDAA010000415        11211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_100023,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000415 DATDAA010000000
VERSION     DATDAA010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 11211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11211
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100023"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VIS73_12325"
     CDS             <1..736
                     /locus_tag="VIS73_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HEY5763986.1"
                     /translation="LQPRQTNQLLSHASHLLSDLTHFAGIVLVPKRAMPKIRHIEFVG
                     LAERRILLILVTADGDVQNRILLTERAYTPSELVTAGNQLTANYAGLDFDQIRSRLRD
                     ELSALRGELHTLMKAAIDGGEGDSDTSTPFVLSGERNLLDIDDFSSNVRRLRKLFDLF
                     EQRSELMRLLDVSEKAAGVQIYIGGESGLAPLDECSVVMAPYTANGKVVGSVGVIGPT
                     RMAYERVIPIVDITARLLGNAMSPKE"
     gene            744..1835
                     /gene="hemH"
                     /locus_tag="VIS73_12330"
     CDS             744..1835
                     /gene="hemH"
                     /locus_tag="VIS73_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY5763987.1"
                     /translation="MSAPTFRHGTPPSTAVVIVNLGTPEAPTKEALRRYLREFLSDRR
                     VVDLPRAIWLPILHGIILNTRPAKSAAKYASIWTPEGSPLAVHTERQAKLLKGSLGER
                     GHHVLVRHAMRYGEPSIPTVLDELAGLNATRILLLPLYPQYAASTSATVADAAFRHLL
                     GYRNQPELRTVRAFCDDPGYIASCVAQIREHWIKHGEPDRLLLSFHGLPKVLLERGDP
                     YYCECQKSGRLIAEGLGIADERLAITFQSRFGRAEWLQPYTAETLKSWGKDGLNRVDV
                     FCPGFVGDCLETLEEIALEAKSDFLAAGGKTFHYISCLNERADWISALADLAERALGG
                     WPTKASIPADAMSRQAARAKALGARNLVS"
     gene            complement(1868..2482)
                     /gene="msrA"
                     /locus_tag="VIS73_12335"
     CDS             complement(1868..2482)
                     /gene="msrA"
                     /locus_tag="VIS73_12335"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY5763988.1"
                     /translation="MNPIRSIFIALAVALSMALPARAAQPGPAQAPAIATFAGGCFWC
                     VEEAFDKIDGVLSTTSGFMGGKVANPTYEQVTSGTTGHREVVRIVFDPARTSYEKLLD
                     SFWRNVDPTQKNGQFCDSGEQYRSEIFVHDDAQKRAAEASRARLNQDSRFAGDIQTII
                     TPASTFYPADDYHQDFHEKHFARYQYYKSACGRVARLRFLWRDR"
     gene            2688..3476
                     /locus_tag="VIS73_12340"
     CDS             2688..3476
                     /locus_tag="VIS73_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY5763989.1"
                     /translation="MKTANTPLRAVIFDVDGTLADTEPEGHRVAFNRAFEEFNLGWYW
                     SVQDYGELLATTGGKERIRRFIDSGAPLPALVDRIGLDTAIARLHALKSEIYAAMVLA
                     GRIPLRPGVLRLIRELRESGIRLAVATTTTPSSLASLIRANFGGDMTKLFDVIGAGDI
                     VPHKKPAPDIYQWVLRRLELPPENCIAIEDSRPGLAAARGAGLKTVVTVSAYTAGDSF
                     PDALAVVSDLGEPDRPLRPIAGTPATGPCIDRAQLERWHRGETP"
     gene            3666..7142
                     /locus_tag="VIS73_12345"
     CDS             3666..7142
                     /locus_tag="VIS73_12345"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEY5763990.1"
                     /translation="MTDRRSARSHKKIRSILVANRGEIAIRVFRAASEMGIRTVAIYA
                     AEDRFALHRFKADESYLVGEGQKPIAAYLDIADIVRIARAAGVDAIHPGYGFLSENPD
                     FAEACAAEGIIFIGPRPEVMRSLGNKVSAREMAIAAGVPVVPATGALPRDIEAVKRLA
                     AEVGYPVMLKASWGGGGRGMRAIETEAELPGLLEVARREAKAAFGNDEGYLEKLVRRA
                     RHVEVQIMGDTHGNIVHLFERDCSVQRRNQKVVERAPAPYLSDEQRSKLCESAVRLAK
                     SAGYTHAGTVEYLMDADSGEFYFIEVNPRIQVEHTVTEEVTGVDVVKAQIRVSEGAAI
                     GDPDGFLPRQEEIRLNGHALQCRITTEDPENGFTPDYGRMTAYRSPAGFGVRLDGGTA
                     YTGAVITPFFDSLLVKTTVWGHSTDEAIARMDRALREFRIRGVSSNLQFLENVIAHPL
                     FRSGECTTRFIDTTPDLFKFKKRRDRATRILRFLGENAINGNPEVAGRKLPELPLPLP
                     HKPVCDLSSPIPPGSRDAFKSLGAQGFAQWMREEKRVLLTDTTMRDAHQSLFATRMRT
                     RDMLEIAPHYARLAPELLSLECWGGATFDVAMRFLKEDPWDRLARLREAVPNILLQML
                     LRGSNAVGYTSYADNVVRYFVEQAAKNGIDLFRVFDSLNWVDNMRVSMDAVIEAGALC
                     EGTICYTGDLFDGARPKYNLKYYVGLAKELEKAGAHILGIKDMAGVCRPRAARELIRV
                     LKQEVGMPIHFHTHDTSGIAAASVLAAVEAGVDAIDGALDAMSGLTSQPNLGAIATAL
                     AGGDRDPGVNLGHLQGLSHYWEGVRRYYSPFEPDIRSGTSDVYQHEMPGGQYTNLREQ
                     ARAMGLEHRWPEISKAYADVNLLFGDIVKVTPTSKVVGDMAIYMVANDLSAAEVADPK
                     KEIAFPESVVSLFRGELGFPPDGFPAELQKKVLRDAKPLSGRAGASIPDVDLDAVRKE
                     AESAIGRHLSDTDLASWLMYPKVFRDFADHHRRYGDVSMLPTQAFFYALPEREEIAVD
                     IDRGKTLVIRQTGTSELVDDEGRIKVFFELNGQPRLVRIAKLGAAPSGPKHPQADESN
                     TNHLGAPMPGSVVTVAVHAGQKVAKGAPLVSIEAMKMETMLSADRDGTIKAVHVKPGD
                     KVAAKDLLVEYA"
     gene            7321..9291
                     /locus_tag="VIS73_12350"
     CDS             7321..9291
                     /locus_tag="VIS73_12350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5763991.1"
                     /translation="MEARTVEPTNDAPPFELRRYFSIASGLIILTLVLLLAFSYRYSE
                     VLEQTGHAGQRNEILARTYANVLWPKFAEFLLRTDLSPAARRTDPETARLHATIAQMS
                     RGIQVVKVKVYNVHGTAIYSSVLDEIGEDKHDNPAFQRALDGELTNELTHRGRMSVSE
                     GEIEDVDVVSTYFPIRQAVNEDPVAVFELYSNVTDSVARIEAITLRLFLALWAVFLLL
                     YLALLAIVGHADRILKRQYSSLKEDETRLLAKAEELQREILERQEVERALRRSEKLAD
                     TANRAKSEFLSSMSHELRTPMNAILGFAQLLDTEPDSPLSQNQRKFVAQITRAGEHLL
                     GLINQVLDLARIESGKLSLSIEPVPLAPLVDECLPLIQSLADEHGIGRITVELGELRV
                     NADHFRVKQVVLNLLTNAIKYNRAGGTVSVTAMHKGSMVRITVEDTGSGIPADRFPEL
                     FQPFNRLVRETSDVEGTGIGLALSKRLVESMAGTIGVDSVVDQGSRFWIELPAAGADA
                     AILSLRNAAAGTAAGALKNASMKDMTVLYVEDNPANRLLMKEMFNRLPAVRLLTASDG
                     ETAIAMARATPFDLVILDINLPGIDGFEVLRRLRRDGLDPAVPVIAVSANAMPHDIER
                     GEAAGFAQYHTKPLQFDEFLRAVSRLLGKSRS"
     gene            9288..10397
                     /locus_tag="VIS73_12355"
     CDS             9288..10397
                     /locus_tag="VIS73_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY5763992.1"
                     /translation="MKDFQLRLSAAPILVVDDIEANVILLENLLRQRGYQQVMSTSDP
                     LQVAPLHRQYNFALILLDLQMPRLDGLGVIRQLRENAAEEHLPVLVITAQTDEKLRLQ
                     ALEMGARDYVTKPFVVAELAHRIRNLLEVELAYRDRLAYADVLEEEVRQRTRELQATQ
                     NEILRRLALAGEYRDNETGNHVMRVSHGSQALARAAGLDETTAELILHASPMHDVGKI
                     GIPDYILLHPGRLQGEELKRMRSHVEIGGRILDGHDAPVMRMAHRIAMAHHERWDGGG
                     YPKGLAGEAIPIEARVVAICDVFDALTSSRPYKAAWTIEAATDYLRSQAGRHFDPQLI
                     EAFLGILPQIIAIRENFIDAPTTDLPIDEQIDIQR"
     gene            10534..>11211
                     /locus_tag="VIS73_12360"
     CDS             10534..>11211
                     /locus_tag="VIS73_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5763993.1"
                     /translation="MTASTPPSPTTSATPPLDSRQERVLLVTLAGIQFCHIVDFMVMM
                     PLGPILLDELAIAAHEFGLLVSAYTFTAAIAGVLVAGFVDRFERKRLLLITFALFALA
                     TLACGLSPDFATLLVARCAAGAFGGVLGAMVHTMVGDLIPYERRGRASGTVMAAFSAS
                     TVAGVPLSLFLANHFGWRAPFVFIALLTIGFLVLGWAKLPEMRGHLADADPAVRSRAR
                     PLAAMGRV"
BASE COUNT         2078 a         3806 c         3367 g         1960 t
ORIGIN      
        1 gctccagccg cggcaaacga accagctact gagtcacgcc tcacatctgc tctccgatct
       61 gacgcacttc gcgggcatcg tgctcgtacc gaaacgcgcg atgccgaaga tccgccacat
      121 cgagttcgtc ggccttgccg agcggcggat cctgttgatc ctggtcaccg ccgatggcga
      181 cgtacaaaac cgcatcctgc tcaccgagcg tgcctacact ccgtccgaac tggtcacagc
      241 gggcaaccag ctgaccgcca attacgccgg cctcgatttc gaccagatcc gcagccgatt
      301 gcgcgacgag ctgtcggccc tgcgcggcga gctgcacacg ctgatgaagg cggccatcga
      361 cggcggcgaa ggcgattcgg acacttccac gcccttcgtc ctctcgggcg agcgcaatct
      421 gctcgacatc gacgacttca gctcgaacgt ccgtcggctg cgcaagctgt tcgatctttt
      481 cgagcaacgt tccgagctga tgcgcctgct cgacgtctcg gaaaaggccg ccggcgtgca
      541 gatctacatc ggcggcgaat ccggacttgc gccgctcgat gaatgcagcg tggtcatggc
      601 gccctatacc gccaatggca aggtcgtcgg ctcggtcggc gtcatcggtc cgacccggat
      661 ggcctacgag cgcgtgattc cgatcgtcga tataaccgcg cgcctgctcg gcaatgcgat
      721 gagccccaag gagtaagccc gccatgagcg ccccaacctt ccgccacggc acgccgccaa
      781 gcacggctgt cgtcatcgtc aacctcggca ctccggaggc accgaccaag gaagctctgc
      841 ggcgctacct gcgcgaattc ctttccgacc gccgcgtcgt cgacctaccg cgcgcgatct
      901 ggttgccgat tctccatggc atcatcctca acacgcggcc agcaaagtca gcggcgaaat
      961 acgcgtcgat ctggactccc gaggggtcgc cactggcggt ccacaccgaa cggcaggcga
     1021 agctgctcaa gggctcgttg ggtgaacgcg gccaccacgt actggtgcgc cacgcgatgc
     1081 gctacggcga accctcgatc ccgaccgtgc tcgacgagct tgccggcctg aatgcgacgc
     1141 gcatcctgct cctcccgctc tacccgcaat acgcggccag cacctcggcg acggttgccg
     1201 acgcggcatt ccgtcatctg ctgggctacc gcaatcagcc cgaactgcga acggtccgcg
     1261 ccttctgcga cgacccgggc tacatcgcct cctgcgtcgc ccaaatccgc gagcactgga
     1321 taaagcacgg cgaacccgac cgtctgctgc tgagctttca cggcctgccc aaggtgctgc
     1381 tcgaacgcgg cgacccctat tactgcgagt gccaaaagag cggaaggctg atcgccgaag
     1441 gcctcggcat tgccgacgag cggctggcga tcacctttca gtcgcgcttt gggcgcgccg
     1501 aatggctgca gccgtacacg gccgagacgc tgaaatcctg gggcaaggac ggcctcaatc
     1561 gcgtcgatgt tttttgccct ggctttgtcg gcgactgcct cgagacgctc gaggagatcg
     1621 cgctcgaagc gaaaagcgat ttcctcgctg ccggcggcaa gaccttccac tacatcagct
     1681 gcctcaatga acgtgcggac tggatttcgg cgctcgccga cctcgccgaa cgggcgcttg
     1741 gtggctggcc aaccaaggcc tcaatcccgg ccgacgccat gagtcgccag gccgcccgcg
     1801 ccaaggcgtt gggcgcgagg aacctcgttt cctagttctc agttaatcga ccgaacccgc
     1861 cggtgggcta gcgatcccgc cagaggaagc gaagccgcgc gacacggccg caggccgact
     1921 tgtagtactg gtagcgcgcg aagtgtttct cgtggaagtc ctggtggtag tcgtcggccg
     1981 gatagaaggt cgacgccggc gtgataatcg tctggatgtc gccggcgaag cggctgtcct
     2041 ggttgagccg ggcgcgtgag gcctcggccg cacgtttctg ggcatcgtcg tgaacgaaga
     2101 tctcgctgcg gtactgctca cccgaatcgc agaactggcc attcttctgc gtcggatcga
     2161 cattgcgcca gaagctgtcg agcagtttct cgtagctggt ccgcgccggg tcaaagacga
     2221 tgcggacaac ctcgcggtgc ccggttgtgc cgctggtcac ctgctcgtag gtcgggttgg
     2281 cgaccttgcc gcccatgaat cccgacgtcg tcgatagcac gccgtcgatc ttgtcgaaag
     2341 cctcctcgac gcaccagaag cagcccccgg caaaagtcgc tatggccggc gcctgggctg
     2401 gtcccggttg tgccgcccgc gccggcagtg ccatcgacaa tgccacggct agcgcgataa
     2461 agatgctgcg gatcggattc acggtggcga ccgttcctga aggtgatgac agttgcttgt
     2521 gactggaaac gcccgtcatt gttcccgcgg ccacgacagg caaccgatcg attgaccgta
     2581 atttttaact aatgctttta gtcattagtt tttgcttttc ctaaccattt cggtcgccta
     2641 gacttcaggt ctcgcacaga agcagcaacg ccatttccca agcctcgatg aaaacagcca
     2701 acaccccgct gcgcgccgtg atcttcgacg tcgacggcac cctcgccgac accgagccgg
     2761 aaggccatcg cgtcgccttc aaccgcgcct tcgaggaatt caatctcggc tggtactggt
     2821 cggtccagga ctacggcgaa ctgctggcga ccaccggcgg caaggagcga atccggcgat
     2881 tcatcgacag cggcgcgccg cttccggcgc tggtcgaccg aatcggttta gacacggcga
     2941 ttgcccgcct gcatgctctg aagagcgaga tctatgcggc aatggtgctt gctggccgga
     3001 tcccgctgcg ccctggcgtc ctgcgcttga tccgcgagct gcgcgagtcc gggattcgcc
     3061 tggcagtcgc cacgacgacg acgccgtcca gcctggccag cctgatccgc gccaatttcg
     3121 gcggcgacat gacgaaactg ttcgacgtga tcggcgccgg cgacatcgtt ccccacaaga
     3181 aaccggcgcc cgacatctat cagtgggtac tcaggcgact ggaactaccg cccgagaact
     3241 gcatcgccat cgaggattcg cgtcccggtc tcgccgccgc ccgcggcgcc ggcctcaaga
     3301 ccgtagtcac ggtcagcgcc tatacggccg gcgacagctt ccccgatgcg cttgccgtgg
     3361 tttcggacct cggcgagcca gaccggccgc tgcggccgat tgcgggcaca ccagctaccg
     3421 ggccatgcat cgaccgggcc cagcttgaac gctggcaccg tggcgagacg ccgtagccag
     3481 cagattgcct cacaggcaca agccataggt gtttctggcc gccctgacat cccggcagga
     3541 gaatacagcg ccacccgact atcggtctga ccactgtacc gactcttgcg attcgactag
     3601 gctggcgtac gcttcgatcg acgctcccca cgccaccccc gttcaactgc cgaaagctat
     3661 cgaccatgac tgatcgccgt tctgcacgct cccacaagaa gatccgcagc atcctcgtcg
     3721 ccaaccgcgg cgagatagcc atccgcgtct tccgcgccgc atcggaaatg ggcatccgga
     3781 ccgtggcgat ctacgccgcc gaagaccgct tcgcgcttca ccgcttcaag gctgacgaaa
     3841 gctatctggt cggagaaggc cagaaaccga ttgccgccta tctcgacatc gccgacatcg
     3901 ttcgtatcgc cagggcggcc ggggtcgatg ccatccatcc gggctacggc tttctttccg
     3961 agaatccgga cttcgccgag gcttgcgcgg cagagggcat catcttcatc gggccgcgcc
     4021 ccgaggtcat gcgcagcctc ggcaacaaag tctcggcgcg cgaaatggcc attgcggcgg
     4081 gcgttcccgt ggtcccggca accggcgccc tgccacgcga catcgaggca gtgaagcggc
     4141 tggcggccga agtgggctac ccagtcatgc tcaaggccag ttggggcggc ggcggtcgtg
     4201 gcatgcgcgc aattgaaacc gaggctgaat tgccgggtct gttagaagtt gcccgccggg
     4261 aggcgaaggc cgcgttcggc aacgacgagg gctacctgga gaaactggtg cgccgggccc
     4321 gtcacgtcga agtgcagatc atgggcgaca cgcacggaaa catcgtccac ctgttcgagc
     4381 gcgactgctc ggtgcagcgg cgaaaccaga aagtcgtcga acgcgccccg gcaccctatc
     4441 tctccgacga gcagcgcagc aagttgtgcg aatcggctgt ccgcctggcg aaatccgctg
     4501 gctacacgca cgcgggcacc gtcgagtacc tcatggacgc cgatagcggg gagttctact
     4561 tcatcgaagt gaatccgcgg attcaggtcg aacacacagt caccgaagaa gtcaccggtg
     4621 tcgacgtagt caaggcccag atccgcgttt ccgaaggcgc agcgatcggc gatccagatg
     4681 gcttcctgcc gcgacaggag gagattcgtc tcaacggcca cgcgctgcag tgccggatca
     4741 ccaccgaaga cccggagaac ggcttcacac ctgactacgg gcgaatgacg gcctaccgca
     4801 gcccggccgg cttcggcgta cgcctcgacg gtggaactgc ctacaccggc gccgtcatca
     4861 ccccgttctt tgactcgcta ttggtcaaga ccaccgtctg gggccattcg accgacgagg
     4921 caatcgcgcg aatggaccgc gcgttgcgtg aattccgcat ccgcggcgtt tcgtcgaacc
     4981 tgcagttcct cgagaacgtg atcgcccatc cgctgttccg cagcggcgaa tgcacgaccc
     5041 gtttcatcga caccacgccc gacctgttca agttcaagaa gcgccgcgac cgcgcgacgc
     5101 gaatcctgcg cttccttggc gagaacgcca tcaatggcaa tcccgaggtc gccggccgca
     5161 agctgcccga actgccgttg ccgctaccgc acaagccggt ctgcgatctc tcgtcgccga
     5221 ttccgcctgg ctcgcgcgat gccttcaagt cgcttggtgc gcagggtttc gcacagtgga
     5281 tgcgcgagga aaagcgtgtc ctgctgaccg atacgacgat gcgcgacgcg caccagtcgc
     5341 tgtttgcgac gcgcatgcgc acgcgcgaca tgctcgagat tgcgccgcat tacgcccgcc
     5401 tcgcccccga gctgctctcg ctcgagtgct ggggtggcgc gaccttcgac gtcgcgatgc
     5461 gcttcctcaa ggaagacccg tgggatcgcc tggcccgcct gcgcgaagcc gtccccaaca
     5521 tcctcctgca aatgctcttg cgcggctcaa acgcggtcgg ctatacgagc tatgcagaca
     5581 acgtcgtgcg ctatttcgtc gagcaggcgg caaagaacgg catagatctg ttccgggtat
     5641 tcgactcgct taactgggtc gacaacatgc gcgtgtcgat ggacgccgtc atcgaagccg
     5701 gcgccctgtg cgaaggaacc atctgctaca ccggcgacct gttcgacggc gctcgcccga
     5761 agtacaacct caagtactac gtcgggctcg ccaaagaact ggagaaggcc ggcgcgcaca
     5821 tccttggcat caaagacatg gccggcgtct gccgcccgcg cgcagcccgc gaactgatcc
     5881 gcgtgctcaa gcaggaagtc ggtatgccga tccatttcca tacccatgac acctcgggca
     5941 tcgccgccgc cagcgtgctc gctgccgtcg aagccggcgt cgatgcgatc gacggcgcgc
     6001 tcgacgccat gtccggactc acctcgcagc ccaacctcgg ggcgattgcc acggcgctcg
     6061 ccggcggcga ccgcgatccg ggcgtgaatt tggggcacct gcagggtctg tcgcattatt
     6121 gggaaggcgt tcggcgctac tactcgccct tcgagccaga catccgctcg ggcacttcgg
     6181 atgtctatca gcacgagatg cccggtgggc agtacaccaa cctgcgcgaa caggcgcgag
     6241 cgatgggtct cgagcaccgc tggccggaga tttccaaggc ctacgccgac gtgaacctgc
     6301 tcttcggcga catcgtcaag gttacgccga cctcgaaggt ggtcggcgac atggcgatct
     6361 acatggtcgc caatgacctg agcgcagccg aagtcgccga tccgaagaag gagatcgcct
     6421 tcccggaatc ggtagtgtcg ctgtttcgcg gcgaactggg tttcccgccc gacggttttc
     6481 cggccgaatt gcagaagaaa gtgctgcgcg acgccaaacc gctgtcgggc cgcgccggcg
     6541 ccagcatccc cgacgtcgac ctcgatgccg tccgcaagga ggccgaaagc gccattggtc
     6601 gccatctttc cgacaccgat ctcgcctcgt ggctcatgta tcccaaggtc ttccgcgact
     6661 ttgccgatca ccatcgccgc tacggcgacg tctcaatgct gccgacgcag gcctttttct
     6721 acgcgctgcc cgagcgcgag gagattgccg tcgacatcga tcgcggcaag accttggtca
     6781 ttcgtcagac tggcaccagc gaactcgtcg acgacgaggg acgaatcaag gtcttcttcg
     6841 aactcaacgg acagccgcgc ctggtgcgga tcgctaagct tggcgccgca cccagcgggc
     6901 ccaaacatcc gcaggccgat gagtcgaaca ccaaccatct gggcgcaccg atgccgggca
     6961 gcgtcgtcac ggtcgccgtc catgccggcc agaaagttgc caagggcgcg ccgctggttt
     7021 cgatcgaagc gatgaagatg gagaccatgc tgtctgcgga ccgcgacggc acgatcaaag
     7081 cggtgcatgt caagcctggc gacaaggtgg cggccaagga cttgctggtc gagtacgcct
     7141 gagcggggcc cggcggagcg gccggcgtga cgccgcccgc ccgccgcgat tgtcgatgag
     7201 cctgatggcg cacatggcaa acgaatgtct ttgggtgtag cgttgcagcc atagggaacc
     7261 attccgcccc agccatcaac aagccagtcc ttgcccgaag caaggacaga gatcgtcatc
     7321 atggaagcgc gtacggtcga accgacgaac gacgcaccgc cgtttgaact gcggcgttac
     7381 ttctcgatcg ccagcggcct catcatcctc acgctggtcc tgttgctggc gttctcctac
     7441 cgctacagcg aagtgcttga gcagaccggg catgcgggcc agcgcaacga aatcctcgcg
     7501 cgcacctacg ccaacgtgct ctggccaaag ttcgccgagt tcctgctgcg cacggacctc
     7561 tcccccgccg caagacggac cgaccccgaa acagctcgcc tgcacgcgac gatcgcccaa
     7621 atgtcgcgcg gtattcaggt agtcaaggtc aaggtgtaca acgtccacgg cactgctatc
     7681 tattcctccg tcctcgacga gatcggcgag gacaagcacg acaacccggc tttccagcgc
     7741 gctctcgacg gtgagctgac caacgaactg acgcaccgag gccgcatgag cgtctccgag
     7801 ggcgagatcg aggatgtcga cgtcgtttcg acctatttcc cgatccgaca ggctgtgaac
     7861 gaggacccgg ttgccgtgtt cgagctgtac tccaacgtaa ccgactcggt cgcccgcatc
     7921 gaagccatca cgctgcggct gttcctcgca ctgtgggcgg tctttctgct gctttatctg
     7981 gcgctgctgg cgatcgtcgg ccatgctgac cggattctca agcgccagta cagttcgctg
     8041 aaggaagacg aaacccgcct ccttgcgaag gccgaagaac tccagcgcga gattcttgag
     8101 cgtcaggaag tcgaacgcgc actgcgccgc agcgaaaagc tggccgacac cgcaaaccgc
     8161 gccaagtccg agtttctgtc gtctatgagc cacgagctgc gcacgccgat gaacgcgatc
     8221 ctcggtttcg cgcaactgct ggacaccgaa cccgattcgc cgctgtcgca aaaccagcgc
     8281 aagttcgtcg cgcagatcac ccgcgccggc gaacacctgc tcgggttgat caaccaggtg
     8341 ctcgatctgg ctcgaatcga gtcgggcaag ctgtcgctgt cgatcgaacc ggtgccgctg
     8401 gcacccctgg tcgacgagtg cctgccgctg atccagagtc tcgcagacga gcatgggatc
     8461 ggcaggatta ccgtcgagct tggcgagctt cgcgtaaacg ccgaccactt ccgcgtcaag
     8521 caggttgtgc tgaacctgct gaccaacgca atcaagtaca accgcgcagg cggcacggtc
     8581 tcggtcacgg caatgcacaa gggatcgatg gttcgcatca ccgtcgaaga cacgggcagc
     8641 ggcattcccg ccgaccgctt tcccgagctg ttccagccct tcaaccgact cgttcgcgaa
     8701 acgtctgatg tcgaaggcac cggtatcggt ctggcgctca gcaagcggct ggtcgagtcg
     8761 atggcgggga ccatcggtgt cgacagcgtt gtcgaccagg gcagccgctt ctggatcgaa
     8821 ctccccgccg cgggcgccga tgccgcgatc ctgtcgctgc gcaatgccgc ggcaggtacg
     8881 gcggcaggcg cgctgaaaaa cgccagtatg aaagacatga ccgtactcta cgtcgaggac
     8941 aaccccgcca atcggctgct gatgaaggag atgttcaatc gcctgccggc ggttcggctg
     9001 ctcaccgcca gcgacggaga gaccgccatc gccatggcgc gtgcgacccc gttcgatctg
     9061 gtgattctcg acatcaacct ccccggtatc gatggcttcg aagtgctacg gcggctgcgt
     9121 cgcgacggcc ttgacccggc ggtgccggtc atcgccgtca gcgccaatgc catgccgcac
     9181 gacatcgaac gtggcgaggc cgcaggcttc gcccaatacc acaccaagcc gcttcaattc
     9241 gacgaattcc tgcgcgccgt atcgcggctg ctcggaaaat cgagatcatg aaggacttcc
     9301 agttaaggct gtcggcagcg ccgatcctcg tggtcgacga catcgaggcc aacgtcatcc
     9361 tcctcgagaa cctgctgcgc cagcgcggct accagcaggt catgagcact tccgacccgc
     9421 tgcaggtggc gccactacat cgccagtaca acttcgcgct gattcttctt gatttgcaga
     9481 tgccacggct tgatggcctc ggcgtgattc gccagttgcg cgaaaacgct gcagaggagc
     9541 atctgccggt actcgtcatc accgcgcaga cagatgagaa gctgcgtctg caggcccttg
     9601 agatgggcgc gcgcgattac gtcaccaagc ccttcgtggt cgccgagttg gcccaccgga
     9661 tccgcaacct gctcgaggtc gagctcgcct atcgcgaccg cttggcctac gccgacgtgc
     9721 tcgaagaaga agtgcggcag cgcacgcgtg aactgcaggc gacccagaac gaaatcctcc
     9781 gccgcctggc gcttgccgga gagtaccgcg acaacgagac cggcaatcac gtcatgcgcg
     9841 tcagccacgg ctcgcaggcg ctggcccgcg ccgccgggct cgacgaaacc acggcggaat
     9901 tgatcctgca cgcgagcccg atgcacgacg tcggcaagat cggcattccc gattacatcc
     9961 tgctccaccc gggccggctg cagggcgaag aactgaaacg catgcgcagc cacgtcgaga
    10021 tcggtgggcg aattctcgat ggacacgacg caccggtgat gcgaatggca catcgcatcg
    10081 ccatggccca tcacgaacgc tgggacgggg gcggttaccc caagggtctc gccggcgagg
    10141 cgattccgat cgaggcgagg gtcgtggcga tctgcgatgt cttcgacgcg ctgacctcgt
    10201 ctcgccccta caaagccgcg tggacgatag aagccgcgac cgactacctg cgctcgcagg
    10261 ccggccggca tttcgatccc caactgatcg aggccttcct cggcatcctc ccgcagatca
    10321 tcgccattcg cgaaaacttc atcgacgccc ccaccaccga cctgccgatc gacgagcaga
    10381 tcgacatcca gcgttgaatc gaccgccacc gccaacacac tggccggatg ggcaatgccg
    10441 cgcgactcct ccgcggcgac cggtcgcgcc ggcgagttag aagtgatccg gctccggcca
    10501 gcgagtatcc tccgcgatcc ggacccgtca ccaatgaccg caagcacccc gccctcaccc
    10561 accaccagcg ctacaccccc gctcgactcg cgacaggagc gggtgctgct ggtaaccctg
    10621 gccggcatcc agttctgcca catcgtcgac ttcatggtaa tgatgccgct cgggccgatt
    10681 ctgctcgatg agctggcgat cgcggcgcac gagttcggac tgctcgtctc ggcctacacc
    10741 ttcacggcgg caattgccgg cgtgctggtt gccggattcg tagaccgctt cgagcgcaag
    10801 cgcctgctgc tcatcacctt cgcactgttc gccctggcca cactggcctg cggcctgtcg
    10861 cccgatttcg caacactgct cgtcgcccgc tgcgcggccg gcgcctttgg cggcgtcctt
    10921 ggcgctatgg tccacaccat ggtcggcgat ctgattccct acgaaaggcg cggccgtgcc
    10981 agcggcacgg tcatggctgc attctctgca tcgacggtgg ccggtgtgcc gctgtcgctc
    11041 tttctcgcca accatttcgg ctggcgcgca ccgttcgtct tcatcgcgct gctgacgatc
    11101 ggctttctcg tactgggatg ggcgaaactg cccgagatgc gcggccatct tgccgacgcc
    11161 gaccccgcag tgcgcagccg cgcccggccg ctggcggcga tgggccgtgt g
//
LOCUS       DATDAA010000416         5214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_30085,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000416 DATDAA010000000
VERSION     DATDAA010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30085"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VIS73_12365"
     CDS             complement(<1..243)
                     /locus_tag="VIS73_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763994.1"
                     /translation="MTLLAALAICAQAAESVNFRDAVSARFGREQLHFFDKLIAAKRE
                     APSSPVKSLIESSLWVKGQQNPPPLNMTPVGLGAARA"
     gene            complement(270..800)
                     /locus_tag="VIS73_12370"
     CDS             complement(270..800)
                     /locus_tag="VIS73_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5763995.1"
                     /translation="MFSPEERRRFRFQLASSCRSVAGVVLFACAANAAVADNGTKALL
                     ERALAAETESLAILLDHHRLEEDLLSPDVDRLGVFLTLPPGLAAEVEQVQLLVDASSH
                     NPALALPKSRLPLTVPLFVQRVGPGAHSVRLDLRLAGSRGVISQSVSVDKPAGARFVE
                     FRLQGGRSPQLSVVQW"
     gene            complement(863..1474)
                     /gene="metW"
                     /locus_tag="VIS73_12375"
     CDS             complement(863..1474)
                     /gene="metW"
                     /locus_tag="VIS73_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEY5763996.1"
                     /translation="MTVFERNDYQVIARWIGSGERVLDLGCGDGALLRELTAAKNIVG
                     YGVEKDPANVQACIVNGVNVIQSDLEDGLRGFEDQFFHHVVMSLSLQTVRQTQLLLRE
                     MLRVGREAVVSFPNFAYRPNRESIAIGRMPVSEALPYQWFDSPNVRFFTIADFENLCA
                     KDGIEIREALFFDDDQPVTEDPNLNADVAVYRLGRPKGVAGDG"
     gene            complement(1471..2598)
                     /locus_tag="VIS73_12380"
     CDS             complement(1471..2598)
                     /locus_tag="VIS73_12380"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEY5763997.1"
                     /translation="MGIDGSVGVVTPRTLRCDSGIRLQGGAFLPGFDLVFETYGELNA
                     AKSNAVLVCHALSGNHHVAGVYADQPKNEGWWDNLVGPGKPLDTNRFFVVGVNNLGGC
                     YGSTGPASIDPATGKPWGARFPVVTVEDWVDAQAWLADQLGIDAWAAVIGGSLGGMQA
                     LDWTLRYPERIRHALVIASTAKLSAQNIAFNEVARQAILTDPDFHGGDYYAHGVVPVR
                     GLRLARMVGHITYLSDDQMAAKFGRQLRDGTHKYNYDVDFEIESYLRYQGDRFTTYFD
                     ANTYLITTKALDYFDPARDYDGNLAAALARAKADFFVASFSTDWRFAPARSRELVYAL
                     VHNTRNVSYVEIDSSAGHDSFLLADAVYHDAVRAYFGRITL"
     gene            complement(2776..4101)
                     /locus_tag="VIS73_12385"
     CDS             complement(2776..4101)
                     /locus_tag="VIS73_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEY5763998.1"
                     /translation="MTRNRKTLALLPLAAALQLLPAGAAVAESIVEAWRDALASDPVY
                     TSARASRAASAERSTQGRAGLLPNVTLTANAVRNDIEVNGISDSSTSRAYELRLTQPL
                     WRAQNWIAYDQAKLTAAQADAEFRQAEQDLILRVAQAYFDVLVAEENLRVVESQKLAI
                     GQQLELAKKSYEVGTATITDVHEAQSRFDLSTAQGIAAENDLAVRQRALAVVTGKEYA
                     AFVPLREPVTLASPEPMDMTSWSAAAERDNPAVVAQQAQTEIASLEADRARAGHQPTL
                     DLIATAGQSDRGRSQFTGTLSDTESRTIGVYLTVPLYQGGGTQSAANEAAYRRDAAVA
                     GLDAARRNAAQGARQAYLGVVSGLAQIRALEAAVVSSEAALASNRLGYEVGVRINIDV
                     LNAEQQLSAARRDLVKARYDTLLAQMQLKAAVGALDEAVLQQTAALMSR"
     gene            complement(4125..4448)
                     /locus_tag="VIS73_12390"
     CDS             complement(4125..4448)
                     /locus_tag="VIS73_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY5763999.1"
                     /translation="MQQLSPRELADWLNASDRLRPQLLDVREPWEAEICRIEGAELIP
                     MRTIPQRIGELASDSPIVVICHHGVRSLQVALYLARAGFAEVHNLQGGMAAWADQVAP
                     AMARY"
     gene            complement(4457..5113)
                     /locus_tag="VIS73_12395"
     CDS             complement(4457..5113)
                     /locus_tag="VIS73_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY5764000.1"
                     /translation="MDFDKARFNMVEQQIRPWEVLDPTVLGLLMTVRREDFVPTPYRQ
                     LAFADVEIPLSDKAVMLAPKIEAKMLQDVGLKSTDRVLEIGTGSGYMAALLASLTQEV
                     TTVEIDRGLAEQARKNLEAAGIANVRVELGDGLKGWPAKAPYDVVVVSGGVAAVPEAL
                     FEQLKPNGRLIAIVGTAPLMRVELYTRDAEGAITSVTLFETETPMLANASRPAPAFAF
                     "
BASE COUNT          922 a         1675 c         1718 g          899 t
ORIGIN      
        1 agcccgcgcc gcgcccagcc cgaccggcgt catgttgagc ggcggcggat tctgctggcc
       61 cttcacccac agcgaggatt cgatcaacga cttgaccggt gaactcgggg cttcgcgttt
      121 ggccgcaatc agcttgtcaa agaagtgcag ctgttcgcga ccgaaacgtg cggaaactgc
      181 gtcgcggaaa ttgacggact cggccgcctg agcgcagatc gcaagcgccg cgagcagcgt
      241 catgagaaac aactgaaacc gtgaaatgct taccactgga ctaccgacag ctgcgggctc
      301 cgcccaccct gcaagcggaa ctcgacgaaa cgcgcaccgg ccggcttgtc gaccgacacc
      361 gactggctga tcacgccgcg gctgcctgcg agccgcagat cgagcctgac cgagtgcgcg
      421 ccgggtccga cgcgttgcac gaagagcggc acggtcaggg gcaagcggga cttcggcagg
      481 gccagcgccg ggttgtggct cgaggcgtcg accagcagtt gcacctgttc gacctcggcc
      541 gccaggcccg gcggcaaggt caggaagacg ccaaggcgat cgacatctgg ggagagcagg
      601 tcctcttcga gccggtggtg gtcgaggagg atcgccaggc tctcggtttc ggctgccagc
      661 gcccgctcaa gcagagcctt ggtgccgttg tcggcgaccg cagcgttcgc cgcgcaggcg
      721 aacaggacca ccccggcgac ggaccggcag gaactggcga gctggaaccg gaaacgacgg
      781 cgttcttctg gtgagaacac tcagaacctc cctgatgcag gaggctagca gaatcgatgc
      841 cagataaatt atcccgctgc gactagccgt cgcctgccac gcctttcggc ctgccgaggc
      901 gatagacagc gacatcggcg ttgagattgg gatcctcggt gacaggctga tcgtcgtcga
      961 agaacagcgc ttcgcggatt tcaatgccat ctttggcaca gagattctca aagtcggcaa
     1021 tcgtaaagaa gcggacgttg ggcgagtcga accactgata gggcagcgcc tcggaaaccg
     1081 gcatacgccc gatcgcaatc gactcgcggt tcggccgata ggcgaaattc gggaacgaga
     1141 ccacggcctc gcggccaacg cgcagcattt cgcgcagcag cagctgcgtc tggcgaaccg
     1201 tctgcagcga cagcgacatc accacgtggt ggaagaactg gtcctcgaac ccgcgcaatc
     1261 cgtcttcgag gtcgctctgg atcacgttga caccgttgac gatgcaggcc tgcacgttgg
     1321 ccgggtcctt ttcgacaccg tagccgacga tgtttttggc cgcggtaagt tcgcgcagca
     1381 gcgcgccgtc gccgcaaccg agatcgagca cgcgttcgcc gctgccgatc cagcgggcga
     1441 tgacctgata atcgttgcgc tcgaagaccg tcatagcgtg atcctcccga agtaggcgcg
     1501 caccgcatcg tggtagacgg catcggcgag caggaaggag tcgtggccgg cgctcgagtc
     1561 gatttcgacg taactgacat tgcgcgtgtt gtgtacgagc gcgtagacga gttcgcgcga
     1621 gcgcgccggc gcgaagcgcc agtcggtcga aaagctggcg acgaagaagt cggccttggc
     1681 cctcgccagc gccgccgcga ggttgccgtc gtaatcgcgc gcgggatcga agtagtcgag
     1741 cgccttggtc gtaatcaggt aggtgttagc gtcgaaatag gtggtgaacc ggtcgccctg
     1801 gtaacgcagg taggactcga tttcgaagtc gacgtcgtag ttgtacttgt gcgtgccgtc
     1861 gcgcagctgg cgcccgaact tggcggccat ctggtcatcg gacagatagg tgatgtggcc
     1921 gaccatgcgc gccagccgca gcccgcgcac cggaaccacg ccgtgcgcgt agtaatcgcc
     1981 gccatggaag tcgggatcgg tgaggatcgc ctgccgcgcc acttcgttga aggcaatatt
     2041 ttgcgccgaa agcttggcgg tcgaggcaat gaccagcgcg tgacggatgc gctcggggta
     2101 gcgcagtgtc cagtcgagcg cctgcatgcc acctagactg ccgccgatga ccgcggccca
     2161 ggcgtcgatg ccgagctgat cggcaagcca ggcctgcgcg tcgacccagt cctcgaccgt
     2221 gacgaccggg aagcgggcac cccagggctt gccggtcgcc gggtcgatcg aagccggccc
     2281 cgtcgagcca tagcagccac cgagattgtt gacgccgacg acgaagaaac gattggtatc
     2341 gagtggcttg ccgggtccga ccaggttgtc ccaccagcct tcgttcttcg gctggtcggc
     2401 gtagacgccg gcgacgtggt ggttgcccga gagcgcatgg cagaccagca cggcgttgct
     2461 cttcgctgcg ttgagttcgc cgtaggtttc gaagacaaga tcgaaccccg gcaggaaggc
     2521 gccgccctgc aagcggattc ccgaatcgca gcgcaaagtc cgtggcgtca ccaccccgac
     2581 cgaaccgtca atgcccattc tgattgccca aacaataaac ccagcctgcg acgacggttt
     2641 cgcgcggact gggtcctcgc tttagccgta cttgttaagc gcccgcaagc tgatcgtcaa
     2701 atcggcgctg aacgcgaact tatcgcgtcg gaaagaaatg gtcaaacccg gtaccgcgcc
     2761 gccggtcgct cggcgtcagc ggctcatcaa ggccgcggtc tgttgcagca cagcctcgtc
     2821 gagcgcgccg accgccgcct tcagctgcat ctgggccagc agcgtgtcgt agcgcgcctt
     2881 gaccagatcg cgccgcgccg ccgagagctg ctgctcggcg ttgaggacgt cgatgttgat
     2941 gcgcacgccg acctcgtaac cgaggcgatt ggaggcgagc gccgcttcgg aggaaacgac
     3001 ggccgcctcg agtgccctga tctgcgccag tccgctgacc acgcccagat aggcctgccg
     3061 cgcgccctgc gcggcattgc ggcgggcggc gtctagcccg gcaaccgcgg cgtcacgccg
     3121 ataggcagct tcgttcgcag ccgactgggt gccgccaccc tgatagagcg gcacggtcag
     3181 gtagacgccg atcgtccggc tctcggtatc gctcagcgtg ccggtgaact ggctgcggcc
     3241 gcggtcggac tgccctgccg tggcgatcag gtcgagcgtc ggctgatggc cggcgcgcgc
     3301 gcgatcggcc tcaagcgagg cgatctcggt ctgcgcctgc tgcgcaacga ccgccggatt
     3361 gtcgcgctcg gccgccgccg accacgaggt catgtccatc ggctccgggc tagccagcgt
     3421 gaccggctcg cgcagcggaa cgaaagccgc gtattccttt ccggtgacga cggccagcgc
     3481 acgctgcctg accgccaggt cgttctcggc ggcgatgccc tgcgccgtgc tcaggtcgaa
     3541 acgcgactgc gcttcgtgca catcggtaat cgtcgccgtg ccgacttcgt agctcttttt
     3601 ggcgagttcg agctgctgcc cgatggccag cttttgcgac tcgacaacgc gcaggttttc
     3661 ctcagcgacg agtacgtcga aataggcctg cgcaacgcgc aggatcaggt cctgctcggc
     3721 ctgacgaaac tcggcatcgg cctgcgccgc ggtgagcttc gcctgatcgt aggcaatcca
     3781 gttctgcgcc cgccagagcg gctgcgtcag ccgcagttcg taggcacgcg aggtggaact
     3841 gtcgctgatg ccgttgacct cgatgtcgtt gcgcacggca ttggccgtca aggtcacgtt
     3901 cggcagcaat ccggcgcgtc cctgcgtcga ccgttcggca ctggcagcgc gactcgcgcg
     3961 tgcgctggtg tagacgggat cgctggcgag cgcgtcgcgc caggcctcga cgatggactc
     4021 ggccactgcc gcaccggcag gcagaagctg caatgcggcc gcgagcggca ggagggctaa
     4081 cgtctttcga ttgcgtgtca ttacgaatct ccggcttagc agcgtcagta gcgggccatc
     4141 gccggggcga cctggtcggc ccaggccgcc atgccgccct gcaggttatg gacttcggcg
     4201 aagccggcgc gcgccagata gagcgcgacc tgcaggctgc gcacgccgtg gtggcagatg
     4261 acgacgatcg gcgaatcgct cgccagctcg ccgattcgct gcggaatcgt tcgcatcggg
     4321 atcaattccg caccctcgat ccggcagatt tcggcctccc agggttcgcg cacgtcgagc
     4381 agctgcggcc gaaggcgatc gcttgcgttc agccaatcgg ccagttcgcg tgggctcagc
     4441 tgctgcatcg cgtgggtcag aaggcgaagg ccggcgccgg acggctcgca ttcgcgagca
     4501 tcggggtctc ggtctcgaag agcgtcaccg atgtgatcgc accttcagca tcgcgggtgt
     4561 acagctcgac gcgcatcagc ggcgcggttc cgacgatggc aattagccgg ccattgggct
     4621 tgagctgctc gaacagcgcc tcgggtaccg cggcgacacc gccggagacc acaaccacat
     4681 cgtagggcgc cttggccggc cagcccttga ggccgtcccc cagctcgacg cggacgttgg
     4741 cgatgccggc ggcctcgaga ttcttccgtg cctgctcggc gagaccgcgg tcgatttcga
     4801 cggtggtgac ttcctgcgtc agcgaggcga gcagagcagc catgtagccg ctgccggtgc
     4861 cgatttcgag gacgcggtcg gtagacttca gcccgacgtc ctgcagcatc ttggcttcga
     4921 tcttcggcgc cagcatcacc gctttgtcgg acaacgggat ctcgacgtcg gcaaaggcca
     4981 gctgccggta gggagtcggc acgaaatcct cgcgccgtac ggtcatcaag agcccgagca
     5041 cggtggggtc gagcacctcc cacggtcgga tctgctgctc gaccatgttg aagcgcgcct
     5101 tgtcgaaatc cattgtctgt tccatttcag taacctcgtc caatattagc aaattctgat
     5161 ttgcatgtcc ccagcagggc gcgattatac gttgcagtga attgacgtcc cggc
//
LOCUS       DATDAA010000417         5496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_170400,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000417 DATDAA010000000
VERSION     DATDAA010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170400"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="VIS73_12400"
     CDS             <1..1492
                     /locus_tag="VIS73_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5764001.1"
                     /translation="DHSVRHFENRAIVAILSSQGVYCRDDSETAKRFLGTSGVLRDIT
                     ERKKAEETIAYQAFHDLLTGLPNRVLFRDRLDLAINQAKRRRHRVGVMYLDIDRFKVV
                     NDTYGHAEGDELLRYFARRVRNCLRGSDTLSRQGGDEFTILLPDLETNDSIEAIAEKI
                     LNELRPPFSVGGKDFTTSASIGIAVYPENGHGAEELLRNADIAMYEVKHRGKNGHLRY
                     AADMSANHAERIELDNEFRIALDMNEFELFFQPQISVSQRRVIGVEALVRWRHPSRGL
                     VPPNSFIPLAEENGLIFRLSDWVLDEACRQMAVWSAAGLDDLRVAVNISPLEFEREDF
                     VERVVAPARRHGVNPSRFEIEITENLLVHDAERVTGHIGALREFGMRVSIDDFGTRYS
                     SLNYLRRFSVNAVKIDQSFVRDIKYRDDKSPVISAIVGIARGFDLNVIAEGVETVEQA
                     DALGDLGCDDMQGYLFCKPKPAAELIDFLRTPALPGKLAEQVRRRA"
     regulatory      1571..1643
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            1699..5442
                     /gene="metH"
                     /locus_tag="VIS73_12405"
     CDS             1699..5442
                     /gene="metH"
                     /locus_tag="VIS73_12405"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY5764002.1"
                     /translation="MQPDRTAELKALLARRILILDGAMGTMVQQHHLTEADYRGDRFD
                     DSAKDLKGNNDLLVLTRPDVIGGIHRNYLEAGADLIETCTFNGTRVSQAEYGLEELAY
                     EINVAGARLVRALCDEYTAKNPAKPRFCAGVLGPTSRTLSISPDVNDPGFRNTSFDAL
                     VADYADSAKGLIEGGADILLIETVFDTLNAKAAVFAVEQVFDELGQRLPVMISGTITD
                     ASGRTLSGQTAEAFWNSLAHARPISFGLNCALGAKDLRAYVEELSNVADTFISAHPNA
                     GLPNPLSPTGYDETPEQLASEIREWAKSGLVNIVGGCCGTSPAHIAAIAQAVSDLTPR
                     AIPALEKKLRTAGLEPFNVGADSLFVNVGERTNVTGSKAFARMILEGRFDDALAVARN
                     QVEAGAQVIDINMDEAMLDSLAAMERFLKLIASEPDISRVPIMLDSSKWSVIEAGLKC
                     IQGKGIVNSISMKEGEAEFLRQARLTLRYGAAAIVMAFDEQGQADTFARKTEICARAY
                     KLLTGIGFPPEDIIFDPNIFAIATGIPEHDNYAVDFIEAVRWIKENLPHAKTSGGVSN
                     VSFSFRGNDGVREAIHTVFLYHAIQAGLTMGIVNAGQLGVYDDLDPALRDKVEDVVLN
                     RHPGAGEALVEAAQSVKEGKAKNTGPDLSWREQAVEARLSHALVKGITDFVVADTEEC
                     RAALAAAGQPPLSVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAHLLPYI
                     EAEKQRTGATNKGRVIMATVKGDVHDIGKNIVGVVLSCNGYEVIDLGVMVPCEKILAA
                     AREHEAQVIGLSGLITPSLEEMSHVASEMQRQGFAETGIPLLIGGATTSRPHTAIKIA
                     PHYQGTVVYVPDASRAVGVVTTLLSIDHAAGFKAEIAADQENARATHAAKAGPTLVDL
                     ATARANALRSDWTAAAPACCSTAIKAGFQPVTPVAPKHPGLHVLTPGLAPLREYIDWS
                     PFFQSWDLVGRYPAILDKPEVGETARQLFADAQTMLDQLIAERWLQPRAVFALWPAAS
                     DGDDIVFFEDETRRTERGRWVGLRNQQKQPDGRPNVALADFVAPTDSGVPDWAGAFAV
                     TAGHGIEEHVARFEAAHDDYSAILLKALADRLAEACAEWLHREVRMCYWGYDADERLD
                     NEALIGEQYRGIRPAPGYPACPDHTAKAELFRLLDAPTNAGMKLTESYAMLPAAAVSG
                     FYLAHPQARYFAITRIGDDQLADWARRKGMPVGEAARWLAPLL"
BASE COUNT         1034 a         1892 c         1660 g          910 t
ORIGIN      
        1 ggaccactcg gtccgccatt tcgagaatcg ggcgattgtc gcaatcctca gttcgcaggg
       61 cgtctactgc cgcgatgact cggaaaccgc gaagcgcttc ctcggcactt cgggcgtatt
      121 gcgcgacatc actgaacgca agaaggccga agaaacgatc gcctatcagg ctttccacga
      181 cctgctgacc ggcctaccca accgcgtcct cttccgcgac cgcctcgatc tggcgatcaa
      241 ccaggcgaag cggcggcgcc atcgcgtcgg cgtcatgtac ctcgacatcg accgcttcaa
      301 ggtcgttaac gacacctacg ggcacgccga gggcgacgaa ctgctgcggt actttgcgcg
      361 ccgggtccgc aattgcctgc gcggcagcga cacgctgtca cgccaaggcg gcgatgagtt
      421 caccatcctg ttgcccgacc tcgagaccaa tgactcgatc gaggcgatcg ccgagaagat
      481 cctcaacgag ctgcgcccgc cgttcagcgt cggtggcaag gatttcacca cctctgcgag
      541 catcggtatc gcggtctacc ccgagaacgg gcacggcgcc gaggaactgc tgcgcaacgc
      601 cgacatcgcg atgtacgagg tcaagcatcg cggcaagaac ggccacctcc gctacgccgc
      661 ggacatgagc gccaatcacg ccgagcggat cgagctcgat aacgagttcc ggatcgcgct
      721 cgacatgaac gagttcgagc tcttcttcca gccacagatc agtgtttcac aacgccgcgt
      781 catcggcgtc gaggcattgg tgcgctggcg acacccgagc cgcgggctgg tgccgccgaa
      841 cagcttcatc ccactggccg aagaaaacgg cctgattttc cggctcagcg actgggtgct
      901 cgacgaggcc tgccggcaga tggccgtctg gtcggctgct gggctcgacg acctgcgcgt
      961 cgctgtcaac atctcgccgc tggagttcga acgcgaggac ttcgtcgaac gcgttgtcgc
     1021 cccggcacgc cgccacggcg tcaacccgtc gcgctttgag atcgaaatca ccgagaacct
     1081 gctggtccac gatgccgagc gcgtcaccgg tcacatcggc gccctccgcg aattcggcat
     1141 gcgcgtctcg atcgatgact tcggcacgag atattcctcg ctcaactatc tgcggcgctt
     1201 ctcggtcaat gcggtcaaga tcgaccaatc cttcgtccgc gacatcaagt accgcgacga
     1261 caaatcgccg gtgatcagcg cgatcgtcgg catcgcccgc ggcttcgatc tcaacgtcat
     1321 cgccgaaggc gtcgaaaccg tcgagcaggc ggatgccctg ggcgaccttg gctgcgacga
     1381 catgcagggc tatctgttct gcaagcctaa gccggcggcc gaactgatcg atttcctccg
     1441 cacgccggcg cttccgggca agctcgccga acaagtgcgc cgacgcgcct gaaccgactg
     1501 cggcggggag gccgcccaaa cgattcgatc cgcttggcaa gccgcgaagg cagcaggtaa
     1561 actccagccc attgccgagg agcgttgcga ccccagcccc gctggagcca ggctcggcaa
     1621 tcgaacaacg gcgctcgcaa actttcaggg tttgtcgcgc cgttttgcat tggcgccgcc
     1681 cgcaacggaa ggaccgccat gcagcctgat cgcacagccg aactcaaagc acttctcgcc
     1741 cgccgcatcc tgattctcga tggcgcgatg gggacgatgg tgcagcagca ccatctgacc
     1801 gaagccgatt accgcggcga tcgcttcgac gacagtgcca aggacctcaa gggcaacaac
     1861 gaccttctcg tcctgacccg ccccgacgtc atcggcggca tccaccgcaa ttacctcgag
     1921 gccggcgccg acctcatcga gacctgcacc tttaacggca cgcgcgtctc gcaggcggaa
     1981 tacgggctgg aagagctcgc ctacgaaatc aacgttgccg gcgcacggct agtgcgtgcg
     2041 ctgtgcgacg aatacacggc gaagaacccg gccaagcccc gcttttgcgc cggcgtgctg
     2101 gggccgacct cgcgcacact gtcgatctcg cccgacgtga acgatccggg gttccgcaac
     2161 accagcttcg acgcactggt cgccgactac gccgattcgg ccaaggggct gatcgaaggc
     2221 ggcgcggaca ttctcttaat cgagaccgtg ttcgacacgc tcaacgccaa ggccgcggtg
     2281 tttgccgtcg agcaggtttt tgacgaactt ggccagcgtc tgccggtgat gatttccggc
     2341 acgatcaccg acgcctcggg ccgcacgctc tcggggcaga ccgccgaggc attctggaat
     2401 tcgctggcgc acgcgaggcc gatttccttt ggcctgaact gcgcgcttgg cgctaaggac
     2461 ctgcgcgcct acgtcgaaga actttcgaac gttgccgaca ccttcatttc ggcgcacccc
     2521 aacgccggcc tgcccaatcc gctctcaccg acgggctacg acgagacccc cgaacagctc
     2581 gccagcgaaa tccgtgagtg ggcaaagtcc ggtctggtga acatcgtcgg cggttgctgc
     2641 ggcacttcgc cggcgcacat cgcggcgatt gcgcaggcgg tttctgattt aaccccgcgg
     2701 gccattccgg cactggagaa gaagctgcgc acggcggggc tggaaccgtt caacgtcggc
     2761 gccgactcgc tctttgtcaa cgtcggcgag cgcaccaacg tcaccggctc gaaggccttc
     2821 gcgcgcatga tcctcgaagg ccgcttcgac gacgcactgg cggtggcgcg caatcaggtc
     2881 gaagccggcg cgcaggtcat cgacatcaac atggacgagg cgatgctcga ttcgctcgcc
     2941 gcgatggagc gctttctcaa gcttatcgcc tccgaaccag acatctcgcg cgtgccgatc
     3001 atgctcgact cgtctaagtg gagcgtcatc gaggctggcc tcaagtgcat tcagggcaag
     3061 ggcatcgtca actcgatttc gatgaaggaa ggcgaggctg aattcctgcg tcaggcccgg
     3121 ctcacgctgc gttacggcgc tgccgccatc gtaatggcct tcgatgaaca gggtcaggcc
     3181 gacacctttg cgcgcaagac cgagatctgt gcccgcgcct acaagctgct caccggcatc
     3241 ggcttcccgc ccgaagacat catcttcgac cccaatatct tcgccatcgc caccggcatt
     3301 cccgagcacg acaactacgc tgttgatttc atcgaggcag tgcgctggat caaggaaaac
     3361 ctgccgcacg ccaagacttc tggcggcgtc tccaacgtca gcttcagctt ccgcggcaac
     3421 gatggcgtgc gcgaggcgat ccacacggtc tttctctatc acgcgattca ggcgggcctg
     3481 accatgggca tcgtcaacgc cggccagctc ggcgtctacg acgaccttga tcccgccttg
     3541 cgcgacaagg tcgaggatgt agtgctcaac cgccatccgg gggccggcga ggcactggtc
     3601 gaggcggcgc aaagcgtcaa ggaaggcaag gcgaaaaaca ccggacccga tctctcctgg
     3661 cgcgaacaag cggtcgaggc gcggctctcg cacgcgctgg tcaagggcat caccgacttc
     3721 gtcgtcgccg acaccgagga gtgccgcgcg gcgctggccg ccgccggcca accaccactc
     3781 tcagtcatcg aaggcccgct gatggccggc atgaacgtcg tcggcgacct cttcggcgcc
     3841 ggcaagatgt tcctgccgca ggtagtgaaa tcggcacgcg tcatgaagca ggcggtcgcg
     3901 catctgcttc cctacatcga agccgaaaaa cagcgcaccg gcgcgaccaa caagggccgc
     3961 gtcatcatgg ccacggtcaa gggcgacgtg cacgatatcg gcaagaacat cgtcggcgtc
     4021 gtactctcgt gcaacggcta cgaagtcatc gacctcggcg tcatggtgcc ctgcgaaaag
     4081 attctcgccg cggccaggga acacgaagcg caggttatcg gcctgtcggg cctgatcacg
     4141 ccatcgctcg aagagatgag ccacgtcgcg tccgagatgc agcgtcaggg atttgccgaa
     4201 acgggcatcc cgctcctgat cggcggcgcc accacctcgc gtccgcatac ggcgatcaag
     4261 atcgcgccgc actaccaggg caccgtcgtc tatgtgcccg atgcctcgcg cgccgtcggc
     4321 gtggtcacca cgctgctgtc gatcgaccac gccgccggct tcaaggccga gatcgccgcc
     4381 gaccaggaga acgcccgtgc gacgcatgcg gccaaggccg gcccgacctt ggttgatcta
     4441 gccaccgccc gcgccaacgc gctgcgcagc gactggacgg cagccgcgcc ggcctgctgc
     4501 agcactgcga tcaaggccgg cttccagccg gtgacgcccg tggccccgaa gcatccaggt
     4561 ttgcacgtac tcacaccggg gctcgccccg ctgcgcgagt acatcgactg gagtcccttc
     4621 ttccagagtt gggacttggt cggtcgctac ccggcgatcc tcgacaaacc cgaggtcggc
     4681 gaaacggcgc gacaactctt cgccgatgcg cagacaatgc tcgaccagtt gattgccgag
     4741 agatggctgc agccccgcgc agtgtttgcg ctctggccgg cggcgtccga cggagacgac
     4801 atcgtcttct ttgaggacga gacacgccgc accgagcgcg gccgctgggt cggcctgcgc
     4861 aaccagcaga aacaacctga tggccgaccc aacgtagcgc ttgccgactt cgttgcgccg
     4921 acggactccg gcgtgcccga ctgggccggc gcctttgccg tcaccgccgg ccacggcatc
     4981 gaagaacacg tcgcccgctt tgaagcggct cacgacgact actcggcgat cctgcttaag
     5041 gcgctcgccg accgccttgc cgaagcttgc gccgagtggc tgcaccgcga agtgcgcatg
     5101 tgctactggg gctacgacgc cgatgagcgc cttgacaacg aggcgctgat tggcgagcaa
     5161 taccgaggca tccggccggc accgggctac ccggcctgtc cagaccatac cgccaaggcc
     5221 gagctgttcc gcctgctcga cgcccccacc aacgccggca tgaaactgac cgagagctac
     5281 gcgatgctgc cggccgccgc tgtgtccggc ttttatctgg cgcacccaca agcgcgctat
     5341 ttcgcgatta cgcgaatcgg cgacgaccaa ctcgccgact gggcgcgccg caaaggcatg
     5401 ccggtaggcg aggcggcgcg ctggctcgca ccattgcttt gagcgaatcc cccgttgcac
     5461 acggtgttgc aacacgaccg gtggcgcacc ctcggc
//
LOCUS       DATDAA010000418         4597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_135223,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000418 DATDAA010000000
VERSION     DATDAA010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135223"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..2408
                     /gene="bamA"
                     /locus_tag="VIS73_12410"
     CDS             120..2408
                     /gene="bamA"
                     /locus_tag="VIS73_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEY5764003.1"
                     /translation="MNRHLIAGILTGLFSASVAAIQPFVVRDIRIEGIQRTEAGTVFN
                     YLPVRVGDKFDDERASEAVRALFATGFFTDVRIESEGDVLVVVIAERPAIAQIDFIGL
                     KEFDQEQLKKGLREIGLGESRIFDRSLLEKAEQELKRQYLARGKYAVRIVTTVTPLER
                     NRVSISFSIDEGDVAKIRQINIVGAEAIEEAELLDLFELRTPNFMSWFTRNDQYSKQK
                     LSADLETLRSYYLDQGFLEFAIDSTQVSISADRTDIFITISIREGARFTVSGIKVAGD
                     LVLPDEDYRELVKIKAGDTFSRATLNATNKAIADRLGNEGYAFANVNAVPEIDREKSS
                     VAFTLFVDPGRRAYVRRINITGNTRTGDAVIRREMRQMESAWFDASKISRSRERIDRL
                     GFFTEVTVDTPPVSGTTDLVDVNLNVAEKPTGSLMLGVGFSSSENLVVSGSVSQENLF
                     GTGRNLAVQVNSGKVNRVYSVSYTNPYFTIDGISQGFDIYHRTNDPTSLSVGSFRTKT
                     NGVGLRFGFPIGETQSLGAGLSVEQTETTIFDDSPQRYKDFVDTFGAKTLTVMNTLSW
                     GHDSRDSAYYPTRGGTRRLSGEIGLPAGDLRYYRLTANAQQFFPISRDYTLWTNVELG
                     YADGLGGKPLPFFKNYYAGGIGSVRGYDTASLGPRILNADGTFSNEGGNRRVVGNLEF
                     LTPFPGSGRDRAVRLGYFIDGGMVYGKNQSVDLGELRYSAGVALAWYSPIGPLKFSLA
                     KPLNDEVGDRTQRFQFQLGSVF"
     gene            2423..2938
                     /locus_tag="VIS73_12415"
     CDS             2423..2938
                     /locus_tag="VIS73_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY5764004.1"
                     /translation="MSPISRILLLSAALAISGNVSAAELKVGVVRLDRLLRDAPVAVA
                     AQKRIESEFSAREQEVVKLEKQLKSTQENYEKNAITLSESERRAKERDINDMAREVAR
                     RQREFREDLDLRRNEALKEVIARADQAIKKIAEAEKFDLILQDAVFVSQRLDITDKVI
                     KALGEAKEGGK"
     gene            2943..3983
                     /gene="lpxD"
                     /locus_tag="VIS73_12420"
     CDS             2943..3983
                     /gene="lpxD"
                     /locus_tag="VIS73_12420"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY5764005.1"
                     /translation="MGERRATPGYRLSELAARLGGTVVGDDVLVSCVAPLESADAEAI
                     SFLANPKFRRHLDSTQAGAIIIAPKHADATSRPRILHDNPYACYARVVALLNPPERPA
                     PGAHPTAVIGSAVPASAAIGAGAVIGTEVEIGECVVIGANAVIGNRVVVGEGSFIDSG
                     VVVRHDCRIGQRAIIHSGAVIGADGFGFAKDGDDWVKIPQVGRVVIGDDVEIGACTSI
                     DRGALDDTRIGNGVKLDNQIQIAHNVTIGDYTAIAGCVGIAGSTKIGARCTIGGAAMI
                     IGHLELCDGVHVSAGTLISKSIRKPGHYTGVFPVDSHESWLHNAAQLRNLDALAKRVR
                     ELENRLQQLEQS"
     gene            3989..4423
                     /gene="fabZ"
                     /locus_tag="VIS73_12425"
     CDS             3989..4423
                     /gene="fabZ"
                     /locus_tag="VIS73_12425"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY5764006.1"
                     /translation="MDIREVLDHLPHRYPFLLVDRVIDLVPGESIHAYKNVTVNEPFF
                     VGHFPHHPVMPGVLIMEALAQAAGILSFKTMGSKPTDDSVFYFVGIDGARFKKPVLPG
                     DQLHLHVRVQREMRGIWKYKAEARVDGAIVAEAELMCAKRDL"
     gene            4423..>4597
                     /locus_tag="VIS73_12430"
     CDS             4423..>4597
                     /locus_tag="VIS73_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HEY5764007.1"
                     /translation="MPTRIHPTAIVHPGATIGEGVEIGPYSIIGEHVSIGANTWVGPH
                     VVIEGHTTIGADNR"
BASE COUNT          903 a         1437 c         1386 g          871 t
ORIGIN      
        1 gctccgccct ctgatcgggt gatggaatac gggcagcgcc tcggtctggc cttgctcgtc
       61 atgttgatgg ttttcgcctt ctacaacgat atccatcgtc tggtatccgg ttaattgcca
      121 tgaatcgtca tctgatcgcc ggtatcctca ccggcctttt ttctgcgtcc gtcgcggcca
      181 tccagccctt tgtcgtgcgc gatatccgta tcgagggtat ccagcgcacc gaagccggaa
      241 cggtattcaa ctacctgccg gttcgcgtcg gtgacaagtt cgacgacgag cgtgcctcgg
      301 aagccgttcg ggcgctgttc gccacgggtt tcttcacaga tgtgcgcatc gagtccgaag
      361 gcgatgtcct cgtcgtcgtc atcgccgagc gtccggccat cgcgcagatc gatttcatcg
      421 gcctcaagga attcgaccag gagcagctga agaagggctt gcgcgagatc ggtctcggcg
      481 agtcgcggat cttcgaccgc tccttgctcg agaaggccga acaggaactc aagcgccaat
      541 acctggcgcg cggcaagtac gcggtgcgca tcgtgaccac ggttacgccg ctcgaacgca
      601 atcgcgtctc gatcagcttc tcgatcgacg aaggcgacgt cgcgaagatc cgccagatca
      661 atatcgtcgg tgccgaggca atcgaagaag ccgaactgct cgatctgttc gaactgcgta
      721 cgcccaactt catgagctgg tttacccgca acgaccagta ttcgaaacag aaactgtcgg
      781 ccgacctcga aaccctgcgc tcgtactacc tcgaccaggg cttccttgaa ttcgccatcg
      841 attcgaccca ggtctcgatc tccgccgatc gcaccgacat cttcattacc atctcgatcc
      901 gcgaaggcgc gcgctttacc gtttccggga tcaaggttgc cggcgacctc gtgctgcccg
      961 acgaggacta tcgcgaactg gtgaagatca aggctggcga taccttctcg cgggcgacgc
     1021 tcaatgccac caacaaggca atcgccgacc ggctgggcaa cgagggctac gctttcgcca
     1081 acgtcaacgc cgttcccgag atcgatcgcg agaaaagcag cgtcgccttc acgctgttcg
     1141 tcgacccggg ccgacgcgct tacgtccgcc gcatcaacat cactggcaac acacggaccg
     1201 gcgacgcggt cattcgccgc gaaatgcggc agatggagag cgcttggttc gacgcgagca
     1261 agatctcccg ttcgcgtgaa cgcatcgacc gcctcggctt ctttaccgag gtgaccgtcg
     1321 atacgccgcc agtatcggga acgaccgatc tcgtcgacgt caatctgaac gtcgccgaga
     1381 agcccacggg cagcctgatg ctcggcgtcg gcttctcgag ttcggagaac ctcgtggtct
     1441 cgggctcggt gtcgcaggag aacctcttcg gcaccggaag gaacctggcc gtgcaggtca
     1501 acagcggcaa ggtcaaccgg gtctactcgg tctcgtacac caatccgtat ttcaccatcg
     1561 acgggatcag ccagggtttc gacatttacc accgcaccaa cgacccgact tcgctgtcgg
     1621 tcggcagctt ccgcaccaag accaatggtg ttggtctgcg cttcggtttc ccgatcggcg
     1681 agacgcagtc actgggtgcg ggcctctcgg tcgagcagac cgaaacgacg atcttcgacg
     1741 acagcccgca gcgctacaag gatttcgtcg acaccttcgg cgcaaagacc ctgaccgtga
     1801 tgaacaccct gtcgtgggga cacgacagcc gcgacagcgc ctactacccg acccgcggcg
     1861 gtacccgacg gctgagtggc gagattggcc tgccggccgg tgacctgcgc tattaccggt
     1921 tgaccgcgaa tgcccagcag ttctttccga tttcccgcga ctacacgctg tggaccaacg
     1981 ttgaactcgg ttacgcggac ggactgggcg gaaagcccct gccattcttt aagaactact
     2041 acgccggcgg tatcggctcg gtgcggggct acgatacggc gagcctcggc ccgcgtatcc
     2101 tcaacgccga cggaacattc tcaaacgagg gcggcaatcg ccgggtggtc ggcaacctcg
     2161 aattcctgac cccgtttccc ggtagcggtc gtgaccgggc tgttcgcctc ggctatttca
     2221 ttgatggcgg catggtctac ggcaagaacc agtcggtcga tctcggcgag ctgcgttatt
     2281 cggccggcgt cgcgctggcg tggtattcgc cgatcggtcc gctaaaattc agcttggcca
     2341 agccgctcaa cgacgaagtc ggcgacagga cccagcgatt ccaattccag ttgggaagtg
     2401 tgttttgaca aggagaactg cattgagccc catttccaga attctgttgc tgagcgcggc
     2461 gctcgcgatc agcggcaatg tctcggcggc cgagttgaag gtcggcgttg ttcgcctaga
     2521 ccgcttgttg cgcgatgctc cggtcgcggt tgcggcgcag aaacggatcg agtccgagtt
     2581 ctcggcacgc gagcaggagg tcgtcaagct cgagaagcag ctcaagtcga ctcaggaaaa
     2641 ctacgagaag aacgcgatca cgctgtccga atccgagcgt cgcgccaagg aacgggacat
     2701 caacgacatg gcccgcgaag tcgcacgaag gcagcgcgag tttcgtgaag accttgacct
     2761 tcgccgcaac gaggcgctca aggaagtcat cgcgcgcgcc gatcaggcaa tcaagaagat
     2821 cgccgaagcc gaaaaattcg acctgattct gcaggacgcg gtcttcgtca gccagcggct
     2881 cgacatcacg gacaaggtca tcaaggccct aggcgaagcc aaggaaggcg gcaagtaagt
     2941 ccatgggcga gcgtcgcgcg acaccgggtt accggctgag cgagctcgcc gcgcgcctcg
     3001 gcggaactgt tgtcggtgac gatgtcctcg tttcctgcgt agccccgctc gagagcgccg
     3061 acgcagaggc gatcagcttc cttgccaatc ccaagttccg ccgtcacctt gattcgaccc
     3121 aagccggcgc gatcatcatt gcgcccaaac acgccgatgc gacgagtcgg ccgcggattc
     3181 tccacgacaa cccctacgcc tgctacgcgc gcgtcgtcgc gctgctcaat ccgccggagc
     3241 gtccggcgcc cggtgcccat ccgactgctg tgatcggctc ggcggtgccg gcttcggcag
     3301 ctatcggggc aggggcggtg atcggcaccg aggtcgagat cggcgaatgc gtcgtgattg
     3361 gcgccaatgc ggtcattggc aacagggtcg tcgtgggcga gggcagcttc atcgattccg
     3421 gggtggtggt tcgccacgat tgccgaatcg gccagcgggc gatcatccat tcgggtgctg
     3481 tgatcggtgc cgatggcttc ggcttcgcca aggacggcga cgactgggtg aaaatcccgc
     3541 aggtcggccg ggtcgtaatc ggcgacgacg tcgaaatcgg cgcctgcacc tcgatcgacc
     3601 gtggcgcgct cgacgatacg cggatcggca acggcgtgaa gctggacaac cagatccaga
     3661 tcgcccacaa cgtgacgata ggcgactaca cggcgatcgc gggatgcgtt ggcattgccg
     3721 ggagtacgaa aataggcgcc cggtgcacga taggcggcgc ggcgatgatc atcggccacc
     3781 tcgaactctg cgacggcgtt catgtgtcgg cgggaacgct gatctcgaaa agcattcgca
     3841 agcccggtca ttacaccggt gttttccccg ttgatagcca cgaatcgtgg ctgcacaatg
     3901 ccgcgcagct gcgcaactta gacgcgctgg ccaagcgcgt ccgcgaacta gaaaaccgat
     3961 tacaacaact ggagcagtca tgaccgccat ggacatccgc gaggtgctcg atcacctgcc
     4021 gcaccgctac cccttcctgc tggtcgaccg tgtcatcgat ctggtaccgg gcgagtctat
     4081 ccacgcctac aagaacgtca ccgtcaacga gccattcttc gtcgggcatt ttccgcacca
     4141 tccggtcatg cccggcgtgt tgatcatgga ggcgctggca caggcggcgg gaatcctgtc
     4201 attcaagacc atgggctcca aaccgaccga cgattcagtc ttctactttg tcggcatcga
     4261 cggagcacgg tttaagaagc ctgttttgcc gggcgatcag cttcatctgc acgttcgcgt
     4321 gcagcgcgaa atgcgcggta tttggaagta caaggccgaa gcgcgggtcg atggtgcaat
     4381 tgttgccgaa gcggagctga tgtgcgcgaa gcgagatctc tgatgcccac ccggattcat
     4441 cccacggcca tcgtccaccc cggtgcgacg attggcgagg gggtggagat cgggccctac
     4501 tcgattatcg gcgagcatgt ctcgattggc gccaacacct gggtcggtcc acatgttgtc
     4561 atcgaaggtc acacgaccat cggcgccgac aaccgga
//
LOCUS       DATDAA010000419         7756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_345127,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000419 DATDAA010000000
VERSION     DATDAA010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345127"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(379..966)
                     /locus_tag="VIS73_12435"
     CDS             complement(379..966)
                     /locus_tag="VIS73_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEY5764008.1"
                     /translation="MEHQLPPLPFAPDALAPHMSKETFEYHYGKHHQAYVTNLNNLIK
                     GSEYENLDLEAIVKKAPAGGIYNNAAQVWNHSFFWKCLKPKGGGAPTGALAEAIDKKW
                     GSFDDFKKAFQASAVGNFGSGWTWLVKKGDGSLDIVNTGPAGTPLKTGEGKALLTIDV
                     WEHAYYIDYRNARPKFVETFLNSLANWEFAAANYA"
     gene            complement(1060..2436)
                     /gene="xseA"
                     /locus_tag="VIS73_12440"
     CDS             complement(1060..2436)
                     /gene="xseA"
                     /locus_tag="VIS73_12440"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY5764009.1"
                     /translation="MNETAVRFQSAEQAISVAELNQRVRQLLEQEIPLLWVRGEISNL
                     TFAASGHLYFTLKDEQAQVGCVMFRSRAQMLGWRPAAGQRVEARGRVTLFESGGRYQL
                     QIDALKPAGLGDLHARFIALRDRLAAEGLFDPARKRALPPLPARIGIVTSPQAAALRD
                     VVTTLRRRAPQVDLIVYPTPVQGEAAAAQIIAALRNAERRAECELLILCRGGGSLEDL
                     WCFNDEALARCLRAITIPVVCGVGHETDFTIAEFAADLRAPTPTAAAELACPDSLALR
                     RQISDAQRRLRGALRQRLDNAAQRVDSLARRLRHPAERLLASRQSLAERSARLARGMG
                     ALIAGARQLVYHARIRHQRLDLRTGLLARSRMAHAANERRLLLANQAQCAMRRQQLGA
                     LAARLRSLDPRQVVERGYAIVTDPKGAVLSDAQAVELNQQIHVNLRNGCLLASVDNKL
                     PPQLPTEE"
     gene            2785..3399
                     /locus_tag="VIS73_12445"
     CDS             2785..3399
                     /locus_tag="VIS73_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY5764010.1"
                     /translation="MFAIIQAAGWPIWPLLFASVVAVALIIERSVSLRRVKIVPPGLL
                     ARVVEELRTAQGPALIQLVQNLEAHSPLGRVLAAAVRNRNATRDVMKEAVEEVGRATA
                     HMLERYLTTLGTIASISPLMGLFGTVVGMIEIFGSQSPSGSNPQQLAHGISIALYNTG
                     FGLMIAIPALIFWRHFRALVDSFVVDMEQQAIRLVELLHGERRG"
     gene            3419..3835
                     /locus_tag="VIS73_12450"
     CDS             3419..3835
                     /locus_tag="VIS73_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY5764011.1"
                     /translation="MDFRRARGRDEPEINLIPMIDVLLVILIFLMITTTYSKFSGMEI
                     NLPTADATVKDQLPNEIDVVVSAEGQVMVNRAPVTSGNAERLAEALRRAAGTRKDPVI
                     VINADAKASHQSVVDVMQAAQIAGYPHISFAIQSPR"
     gene            3835..4896
                     /gene="lpxK"
                     /locus_tag="VIS73_12455"
     CDS             3835..4896
                     /gene="lpxK"
                     /locus_tag="VIS73_12455"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY5764012.1"
                     /translation="MRRTDGSFAEQLEQDWYLPRLTPLTSALRPLAVVFGGLAWLRRM
                     AYRAGLLRSQRVGVPVVIVGNITVGGTGKTPLVVWLSEALRAAGLKPGIVLRGYRAGG
                     ADAGAVREVRADSDAASFGDEAVLLARRTSVPVFAGRDRPAAARALRQANPEVDVILS
                     DDGLQHYAMARDFEIAVFDRRGTGNGRLLPAGPLREPVSRLASVDAIVCHACGCEAAY
                     PMASETPLLKMRLAGTVFVNLHDASRRCSAADLAERQCYAIAGTGAPHRFFDALLELG
                     IVGATTRAFADHHRYSAGDLAFAQGGTLLMTEKDAVKCATLAHPDAWYLPVSATFVDD
                     DGRRLLAMVMEKIDGYPPA"
     gene            4877..5056
                     /locus_tag="VIS73_12460"
     CDS             4877..5056
                     /locus_tag="VIS73_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY5764013.1"
                     /translation="MDTRLLDILVCPVCKAELDYRKDARELICKPCRVAYPIRDDIPI
                     MLEDEARRLEESEAP"
     gene            5053..5823
                     /gene="kdsB"
                     /locus_tag="VIS73_12465"
     CDS             5053..5823
                     /gene="kdsB"
                     /locus_tag="VIS73_12465"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEY5764014.1"
                     /translation="MTTRFHAVVPARFGSTRLPGKPLIDLCGKPMVVRVAERAAASGA
                     ATVTIATDDERVLTVAEAHGIDCLLTRTDHPTGTDRLGEVVARKGWDSETLVVNVQGD
                     EPLIDPALIAHAAAMLADSGADIATLAHPIVDVGEFFNPGIVKVVLRKNGDASYFSRA
                     PIPYARDAFAKTREALPAGLPALRHVGLYAYRAEFLSAFSTLSPAPAENWESLEQLRA
                     LHHGYRITVGIVDEMPTPGVDTPEDAERIRRLIDQGGD"
     gene            5956..6609
                     /gene="adk"
                     /locus_tag="VIS73_12470"
     CDS             5956..6609
                     /gene="adk"
                     /locus_tag="VIS73_12470"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY5764015.1"
                     /translation="MRLILLGAPGAGKGTQAQAICERYGIPQISTGDMLRAQIKAGTP
                     LGLEAKKHMDGGSLVPDAVIIGMVKERLKQDDCGKGYLFDGFPRTIPQAEAMKAAGVP
                     IDYVLEIDVPDSDIVARMAGRRVHLASGRTYHVSFNPPKVEGKDDMTGEALIQRDDDK
                     EATVKKRLEIYHVQTKPLVDFYSRWAESGDSAAAKVRKVSGVGKVSEITERVFGALA"
     gene            6780..>7756
                     /locus_tag="VIS73_12475"
     CDS             6780..>7756
                     /locus_tag="VIS73_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY5764016.1"
                     /translation="MSVLMLALACAVLAIIYGWISSSQILALSAGNARMQEIAKAIQE
                     GASAYLARQYKTITVVGIVVAVLIAIFLDASIAVGFVIGAVLSGAAGFIGMNVSVRAN
                     VRTAEAARGGIAPALDVAFKGGAITGMLVVGLGLLGVAGYYAALLAFGVEQDVALHAL
                     IGLAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNV
                     GDCAGMAADLFETYAVTIVATMVLGALTAAKVAGFGDSLVLYPLALGAVSIVASIIGC
                     YFVKTSDGGKIMGALYKGLAVAGILALIAYYPVTAWLIGAGDGAQITTGT"
BASE COUNT         1291 a         2399 c         2562 g         1504 t
ORIGIN      
        1 cccatcgtga tatttttcgc gaaccattga atggcctgac ttccgtccgc actctttgat
       61 cggttgaacg gcaggtcatc gactcattgc cgcccgcgtc ccgtcacggg cccaacggct
      121 gcaaaggccg aggaggggcc gctggggctg ccggaaggtg gcgccggaat gaaaaccaca
      181 aagtcgtgaa ttcttgagca acagaaacag ctgtgggcgc caattggcgc ccacaggctt
      241 gggaaatcag tccagaaact gactaagaag cacatccagc caagatcttc acgactttat
      301 tgatcgatgc tcccggcgga gttcacgact ttattgtcag gtttcaggac tttatttgta
      361 agaaacaccg ctgcccgatc aggcgtagtt ggctgccgca aattcccagt tggcgaggct
      421 gttgaggaag gtctcgacga acttcgggcg ggcgttgcgg tagtcgatgt agtaggcgtg
      481 ctcccaaaca tcgatggtca gcagtgcctt gccctcgccg gttttcagcg gggtgccggc
      541 ggggccggta ttgacgatgt cgagcgagcc gtcacccttc ttcaccagcc aggtccagcc
      601 cgagccgaag ttgccgacgg cggaggcctg gaaggccttc ttgaagtcgt cgaaggagcc
      661 ccacttcttg tcgatcgcct cggccagcgc gccggtgggg gcgccgccgc ctttcggctt
      721 caggcacttc cagaagaaac tgtggttcca cacctgcgcg gcattgttgt aaatgccgcc
      781 ggccggggcc ttcttgacga tggcttcgag gtcaaggttc tcatactcgc tgcccttgat
      841 cagattgttg aggttggtca cataggcctg atggtgcttg ccgtagtgat attcaaacgt
      901 ttccttcgac atgtgcggcg caagcgcgtc gggagcaaag ggcagaggcg gaagttgatg
      961 ttccatggtc ggttctccgt attgtgttgt ggtgactgag cgggtgtgtc agggatcggc
     1021 cttgcgccgc aactcccggc cgggagtgtg gcacgaagct cattcttcgg tcggcaactg
     1081 gggcggcagc ttgttatcga cgcttgccag caggcaaccg ttgcgcaagt tgacgtgaat
     1141 ctgctggttt agttccaccg cctgcgcatc gctgagaact gcgcccttgg ggtcggtcac
     1201 gatcgcgtag ccacgctcga cgacctgccg cggatcgagg cttctgagcc gggcggccag
     1261 tgcgccgagc tgctgccggc gcatcgcgca ctgtgcctgg ttggcgagca gcaggcgcct
     1321 ttcgttcgcg gcatgcgcca tgcggctgcg ggcgagcagc cccgtgcgca ggtcgaggcg
     1381 ttggtggcgg atgcgggcgt ggtagaccag ctggcgagcg cctgctatca gcgcacccat
     1441 gcctcgagcg agacgggcgc tgcgttcggc aagggattgg cgcgaggcga gcagccgctc
     1501 ggccggatgg cgcaagcgac gggccagact gtcgacccgt tgcgcggcgt tatcgagacg
     1561 ctggcgcagg gcgccacgca ggcgtcgctg ggcgtcgctg atctgccggc gaagcgccag
     1621 cgagtcgggg cacgccagtt cggcggcggc agtcggcgtc ggcgcacgca ggtcggcggc
     1681 gaattcggcg atcgtgaaat cggtttcgtg gccgacaccg caaacgaccg gaatcgtaat
     1741 cgcgcgaagg cagcgtgcga gtgcttcgtc gttgaagcac cagagatctt ccaggctgcc
     1801 accaccgcgg caaaggatca gcagttcgca ctcggcgcgg cgttcggcgt tgcgcagggc
     1861 ggcgatgatc tgcgccgccg cggcttcccc ctggaccggt gtcggataga cgatcaggtc
     1921 gacctgtggc gcgcggcggc gcagcgtcgt aaccacgtcg cgcagggctg ctgcctgtgg
     1981 cgaggtaacg attccgattc gtgctggcaa cggtggcagc gcgcgcttgc gcgccggatc
     2041 gaacaggcct tcggcggcaa gcctgtcgcg cagcgcgatg aagcgcgcgt gcagatcgcc
     2101 gagccccgcg ggtttcaatg catcgatctg cagctggtag cgaccgcccg actcgaacag
     2161 cgtgacccgg ccgcgcgcct cgacgcgctg gccagctgca ggccgccagc cgagcatctg
     2221 ggcgcgcgag cggaacatca cgcagccgac ctgcgcctgt tcatccttga gcgtgaaata
     2281 gaggtggccg gaggcggcga aggtgaggtt ggagatttcg cctcgaaccc agagcagcgg
     2341 gatttcctgt tccagcagtt gtcggacccg ctggttcagt tcggcaaccg agatcgcctg
     2401 ttcggccgac tgaaagcgca cggcagtttc gttcatgtgc cgattctaga cgcttcgtca
     2461 aggcgtcgtg gagcgggcga agcggtgcgc agtggatgcg ggttgccacg cccaattttt
     2521 tggcggacga ccaaaacccc agtcgccccg gtcgcttaat gaattcaatg acaggcttcg
     2581 cacacggtta tccacagaat ctggggataa ctcggcaggt cgttgccgcg ggcagtcgcc
     2641 ttgccgcggt gacggcgtgc cacggtcttt cctgtccggt ccggtcggtg cggccgggtg
     2701 ctgttgttgc cgagcgcgcg tcgcttgtcc tgcaagcctg cggcgcgcta cagttcgcgc
     2761 cttgatgaat tgagggttgg tgccgtgttc gcaatcattc aggccgctgg ttggccaatc
     2821 tggccgctgt tgttcgcgtc ggtcgtcgcc gtggcgctca tcattgagcg cagtgtcagt
     2881 cttcgccggg tgaaaatcgt tccgcccgga ttgctggccc gagttgtcga agaactgcgg
     2941 acggcacagg gaccggccct gatccagctg gtgcaaaacc ttgaggcgca ttcgccgctc
     3001 gggcgcgtgc tcgccgccgc ggttcgcaac cgcaacgcca cccgcgacgt catgaaggag
     3061 gcggtcgagg aagtcggccg cgccacggcg cacatgctcg agcgctatct gacgacgctg
     3121 ggcacgattg catcgatcag ccccctgatg ggcctgttcg gcacggtcgt tggcatgatc
     3181 gagatcttcg gctcgcagtc accgtcgggc tcgaacccgc aacaactcgc ccacggcatt
     3241 tcgatcgcgc tctacaacac cggtttcggc ctgatgatcg cgatcccggc attgatcttc
     3301 tggcggcatt tccgcgcgct ggtcgattct ttcgtcgtcg acatggagca gcaggcgatt
     3361 cgtctggtcg aactgctgca cggcgagcgc cgcggctgag cggacggcag gcgcgaccat
     3421 ggacttccgc cgtgcccggg ggcgtgacga acccgagatc aatctgatcc cgatgatcga
     3481 cgtcctgctg gtgatcctga ttttcctgat gatcaccacg acctactcga agttttcggg
     3541 aatggaaatc aatctgccga cggccgacgc cacggtcaag gatcagctac ccaacgagat
     3601 cgatgtcgtt gtcagcgccg aggggcaggt catggtcaac cgtgcgccgg taacctcggg
     3661 caacgccgag cgacttgccg aggccctgcg tcgcgcggcc ggcacacgca aggacccggt
     3721 gatcgtcatc aacgccgacg ccaaggcatc ccaccagagc gtcgtcgacg ttatgcaggc
     3781 ggcgcagatc gcgggctatc cgcacatttc cttcgccatt cagtcgccgc gctgatgcgc
     3841 cggacggatg ggtccttcgc ggaacaactc gagcaggatt ggtacctgcc gcgcctgacg
     3901 ccgctgacca gcgctctgcg cccgctcgcc gtggttttcg gcggcctcgc ctggctgcgt
     3961 cgcatggcgt atcgcgccgg cttgctgcgc agccagcgcg tcggggtgcc ggtggtgatc
     4021 gtcggcaaca tcaccgtcgg cgggaccgga aagactcccc tcgtcgtctg gttgagcgaa
     4081 gccttgcgcg ccgcgggtct gaagcccggt atcgtccttc gcggttatcg cgcggggggt
     4141 gccgatgccg gcgccgtgcg cgaggtgcgc gccgattcgg atgcggcgag cttcggcgac
     4201 gaagccgttc tgctcgcccg acgtaccagt gttccggttt tcgccggacg cgaccggccg
     4261 gcggcggcca gggccttgcg ccaagccaac cccgaggtgg acgtgattct cagcgacgac
     4321 ggcctgcagc attacgcaat ggcccgcgat ttcgaaattg cggttttcga ccggcggggc
     4381 accggcaacg gtcggctgct gcccgccggg ccgctgcgtg agccagtttc gcggctggcc
     4441 tctgtggatg cgatcgtctg tcacgcctgc ggctgcgagg cggcgtatcc gatggccagc
     4501 gaaactccct tgctgaagat gcggctggcc ggaacggtct tcgtcaatct gcacgacgcg
     4561 agccgccgct gctcggcagc cgatcttgcc gaacgtcagt gttatgcaat tgccggaacc
     4621 ggtgcgccgc atcgcttttt cgacgccctg ctggaattag gcatcgtcgg ggcgacgacg
     4681 cgcgccttcg ccgatcacca tcgctacagc gcgggcgatc tcgcgttcgc gcagggcggc
     4741 acgctgctga tgaccgagaa ggatgcagta aaatgcgcga cgctcgcgca cccggacgct
     4801 tggtacctac cggtgtcggc gacgtttgtt gacgacgacg gccgccgtct gctggccatg
     4861 gtgatggaga agatcgatgg atacccgcct gcttgacatc ctcgtttgcc cggtctgcaa
     4921 agccgagctc gattaccgca aggacgcgcg ggaactgatc tgcaagccgt gccgcgttgc
     4981 ctatccgatt cgcgacgaca ttccgatcat gcttgaggac gaagcacgcc gcctggaaga
     5041 atccgaagcg ccgtgacaac gcgtttccac gcggtcgtcc ctgcgcggtt tgggtcgacc
     5101 cgtctgcctg gcaagccctt gatcgatctc tgcggcaagc cgatggtggt tcgggtcgcc
     5161 gagcgggcgg ccgccagcgg tgcagcgaca gtcaccattg cgaccgacga cgagcgggtc
     5221 ctgacggtgg cagaggccca cggcatcgat tgcctgctca cgcgaaccga ccacccgacc
     5281 ggcaccgatc gtctgggcga agtcgtcgcc cgcaagggct gggattccga aacgctggtg
     5341 gtcaacgttc agggcgacga gccgctgatc gacccggcgt tgatcgcaca cgcggcggcc
     5401 atgctcgcgg attccggcgc cgacatcgcg acgctcgcgc atccgattgt tgatgtcggg
     5461 gagttcttca atcccggcat cgtcaaggtg gttctgcgca aaaacgggga tgccagctac
     5521 ttcagtcgcg caccgattcc ctacgcccgc gacgcctttg cgaagacgcg cgaggcactg
     5581 ccggccggtc tgccggcgct gcgacacgtc ggtctctatg cctatcgcgc cgaattcctc
     5641 tcggccttct cgaccctgtc gccggcgccg gcagagaact gggagtcgct ggagcaactg
     5701 cgcgccctgc accacggcta tcgaatcacg gtgggaatcg tcgacgagat gccgacaccg
     5761 ggcgtcgata cgcccgaaga tgccgaacgg atcaggcgcc tgattgacca aggcggcgat
     5821 tagcggtaat ttccggcgct cttccgggcc accgtcagcc ctgccgtacc cataatgcga
     5881 cgatcggacg caccagctgc cgtcgccgcg accgacaacg gattcagacg agtattccca
     5941 acgagagggt gagttatgcg attgattctt ttgggcgcgc cgggtgccgg aaaaggtacg
     6001 caggcgcagg caatctgcga acgctacggc attccgcaaa tttccacggg tgacatgctg
     6061 cgggcgcaga tcaaggccgg cacgccgctg ggtctcgaag cgaagaagca catggacggc
     6121 ggcagccttg tccccgacgc cgtcatcatt ggcatggtca aggagcgcct caagcaggat
     6181 gactgcggca agggttacct gtttgacgga tttccgcgga cgattccgca ggccgaggcg
     6241 atgaaggccg ccggtgtgcc gatcgactac gtgctcgaaa ttgatgtgcc cgactccgac
     6301 atcgtcgcgc gcatggccgg gcgccgtgtc cacctggcct cgggccgcac gtaccatgtc
     6361 agcttcaacc cgcccaaggt ggaaggcaag gacgacatga ccggcgaggc gctgatccag
     6421 cgcgacgacg acaaggaagc cacggtgaag aagcgtctgg agatctacca cgtccagacc
     6481 aagccactgg tcgacttcta ttcgcgttgg gccgagagcg gcgattcggc tgctgccaag
     6541 gtgcgcaagg tcagcggagt cggcaaggtt tcggagatca ccgaacgcgt atttggtgca
     6601 ttggcctgac cagaaatcgt cgcggtgaaa cggcgggcgc ttcgggcgcc cgctcgcttt
     6661 tgcaggccgc ccctgtcata atcgcgcggt ttgcccggat gggtttcgcc cggaacggta
     6721 acggcagctt cacagtaatc ggctagaaaa agccgcccac tttaatcagg aggttattga
     6781 tgtccgtatt gatgctcgct ctcgcgtgcg ccgtgctggc cattatctac ggctggattt
     6841 ccagcagtca gattctggct ctgtcggccg gcaacgcccg tatgcaggaa atcgccaagg
     6901 ccattcagga aggtgcttcg gcctatctgg ctcgccaata caagacgatt accgtcgttg
     6961 gtatcgtggt tgcagtgctg attgccattt tcctcgacgc cagcatcgct gttggcttcg
     7021 tgattggtgc ggtgctttcc ggcgctgctg gtttcatcgg catgaacgtc tcggttcgcg
     7081 ccaacgtgcg taccgcagaa gccgcgcgtg gcggcatcgc cccggcgctc gacgtggcct
     7141 tcaagggtgg cgccatcacc ggcatgctgg tggttggcct cggtctcctt ggtgttgccg
     7201 gctactacgc ggccttgctc gcctttggcg ttgagcagga cgttgcgctg cacgccctga
     7261 tcggtctggc tttcggctct tcgctgattt cgatcttcgc gcgactcggt ggcggcattt
     7321 tcaccaaggg cgccgacgtc ggtgctgatc tggtcggcaa ggtcgaagcc ggtattccgg
     7381 aagatgatcc gcggaacccc gccgtgattg ccgacaacgt cggtgacaac gtcggtgatt
     7441 gtgccggtat ggcagccgac ctgttcgaaa cctacgcggt gacgattgtc gccacgatgg
     7501 tgctcggtgc gctgactgcc gccaaggttg ccggtttcgg tgattcgctg gtcctctacc
     7561 cgctggcgct tggtgctgtg tcgatcgttg cgtcgatcat cggttgctac ttcgtcaaga
     7621 cttctgacgg cggcaagatc atgggcgctc tgtacaaggg tctggcggtt gccggcatcc
     7681 ttgcgctgat cgcctactac ccggtcactg cctggctgat cggcgctggc gacggcgcgc
     7741 agatcaccac cggcac
//
LOCUS       DATDAA010000420         8136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_135244,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000420 DATDAA010000000
VERSION     DATDAA010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135244"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VIS73_12480"
     CDS             complement(<1..560)
                     /locus_tag="VIS73_12480"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY5764017.1"
                     /translation="MTARNLLVELFVEELPPKALKHLGEAFAASLAASLRQSGLASAE
                     TAVTAYASPRRMAAHLTGVLAVAADKPLVQKLMPVSVGLDAQGKPTPALLKKLAALGA
                     DESVVAGLRREGVGKAEALFYESVAPGATLADGLQKAIEAALAALPIPKVMSYQLADG
                     WSTVNFVRPAHALVALHGSEVVPVAV"
     gene            complement(561..1502)
                     /gene="glyQ"
                     /locus_tag="VIS73_12485"
     CDS             complement(561..1502)
                     /gene="glyQ"
                     /locus_tag="VIS73_12485"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY5764018.1"
                     /translation="MATDKPSFQDVILRLQNYWSAHGCALLQPYDMEVGAGTSHTATF
                     LRAIGPEPWNAGYVQPSRRPKDGRYGENPNRMQHYYQYQVVLKPAPENILELYLGSLE
                     ALGFDLKQNDVRFVEDDWENPTLGAWGLGWEVWMNGMEVTQFTYFQQVGGIDCKPITG
                     EITYGLERLAMYLQGVENVFDLTWTVGPNGRLSYGDVYHQNEVEQSAYNFEHSDAEFL
                     FTAFGAHEKQAQHLMGAQLALPAYEQVLKAAHTFNLLDARGAISVTERAAYIGRIRNL
                     ARSVAQSYLDSRARLGFPMAPKPWADEVLAQLEKRAA"
     gene            complement(1540..3078)
                     /gene="lnt"
                     /locus_tag="VIS73_12490"
     CDS             complement(1540..3078)
                     /gene="lnt"
                     /locus_tag="VIS73_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY5764019.1"
                     /translation="MPLRRLPRPTELLAAFAAGVLLVAAFAPLAWYPLAPLALAVLYL
                     LGLHQPGTRAAAAIGFAFGLGCFLGGVSWVYVSLSVFGGMVAWLAALSTLVFCMVLAL
                     FPALAVAVFARLSTAGLRVPLFSAAWVLGEWLRGTLFTGFPWLAVGYSQGGDSPLAGL
                     APLFGTYGVSFVTALIAACLGEAVWRWRSGYCPASGHPRWAYAAVPLLIAALLGAGGA
                     VLGILEWTHPAGRPVRVALLQGNIAQDMKWRPESFQTSLRTYDALFDAHPADLTVLPE
                     TALPAFWEALPPAFVESLREQARARGGDVLIGAVAGTGERYTNSVFSVGTAPTQRYDK
                     VHLVPFGEFVPPGFATLMRQLSIPMSGFSAGRADQPPLRVGGTTVAVNICYEDVFGDA
                     LRPGARAAAILVNVSNTAWFGRSLAQPQHLQIARMRARETGRPMLRATNTGMTAIVEP
                     DGRVSAVLEPFVRGALVADVQGRTGETPYLRWGDRPVLIASLLLLAVALIVGRARRLG
                     RAAD"
     gene            complement(3047..3898)
                     /locus_tag="VIS73_12495"
     CDS             complement(3047..3898)
                     /locus_tag="VIS73_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEY5764020.1"
                     /translation="MDSSAERPGFLERLTTLLLREPEDRDQLLRLLHSAYERNLLDAD
                     ALSITEGALAASDTSVRDVMVPRAQMDVVDVDESIGNIVRYALERAHSRFPVTQGSKD
                     NVIGILLAKDLLRLQLEDDFDLRDWLRPAVFIPESKRLNVLLREFRVSRNHMAVVVDE
                     YGGVAGLVTIEDVLEQIVGDIEDEYDFDEAADNIQIDQAGRYRVKAQTEIDAFNAAFG
                     TSLEDPECDTIGGLLLKQFGRVPKRNEIIEVASLRVQILRADSRRLYTLLIDRPRHAA
                     PAPAATD"
     gene            complement(3934..4350)
                     /gene="ybeY"
                     /locus_tag="VIS73_12500"
     CDS             complement(3934..4350)
                     /gene="ybeY"
                     /locus_tag="VIS73_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY5764021.1"
                     /translation="MQYAGDRALAPARDAVRRWARAALAGGAQVTIRFVDEDEGRALN
                     RDYRGKDYATNVLSFPYESEPRVFGDLVLCTTVAAREAVAQGKALEAHLAHLIVHGML
                     HLQAYDHDNDNDAEAMETREREILAALGYDDPYATD"
     gene            complement(4394..5371)
                     /locus_tag="VIS73_12505"
     CDS             complement(4394..5371)
                     /locus_tag="VIS73_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY5764022.1"
                     /translation="MANPQQARRSRPREIVLAPVDNARLANLCGALDEHLRQIESALD
                     VSISRRGERFTLHGDSAQTAKAAEALQTFYASADGGLTVDDIQLGLIELANAPLARAA
                     AGPDQVQLMTRKTELHGRTPRQIDYLKAVAAHDITFAIGPAGTGKTYLAVACAVDALE
                     REHVERIVLTRPAVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLMGFDRVAKLFEKG
                     AIEIAPLAYMRGRTLNHSFIILDEAQNTTPEQMKMFLTRIGIGAKAVVTGDVTQVDLP
                     RGQQSGLIEARHVLSAVRGIGIIEFGAEDVVRHPLVARIVQAYDAFRET"
     gene            complement(5364..6707)
                     /gene="miaB"
                     /locus_tag="VIS73_12510"
     CDS             complement(5364..6707)
                     /gene="miaB"
                     /locus_tag="VIS73_12510"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY5764023.1"
                     /translation="MKKLYIKTFGCQMNEYDSDRIADVLAAGEGVAKTDRPDDADIIV
                     FNTCSIREKAQEKVFHELGRARLLKLQKPDLLIGVGGCVASQEGEAIVRRAPYVDVVF
                     GPQTLHRLPQLIAQRRSEGRAAVDVSFPEIEKFDAMPAPTVEGGTAFVSVMEGCSKFC
                     TYCIVPFTRGDEVSRPLADVLAEVERLTVQGVREITLLGQNVNAYRGEMDDGEPADLA
                     LLIELLAEMPAVERIRFTTSHPCEMNARLIETFARVPKLVSHLHLPVQSGSDRVLAAM
                     KRGYTILEYKSVVRKLRAVRPDLSLSTDFIVGFPGETEAEFEATMKLIDEIGFDASFS
                     FVFSPRPGTPAADMADSTAEAVKLARLARLQKRIDELAATVSQGMVGSIQRVLVEGVS
                     KKHAEELAGRTDNNRIVNFAGNPRLIGHFVPVTVTAALPHSLRGEVLTREAVANG"
     gene            6809..6885
                     /locus_tag="VIS73_12515"
     tRNA            6809..6885
                     /locus_tag="VIS73_12515"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6843..6845,aa:Met,seq:cat)
     gene            6981..7856
                     /locus_tag="VIS73_12520"
     CDS             6981..7856
                     /locus_tag="VIS73_12520"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY5764024.1"
                     /translation="MTAAILHIDLDALPPLSPLAGKLLRLDWNDEASDRHLVEIIRSE
                     PQLSARIVGLANSVAFAAPGGRPVIAVEACLQRIGVRRAALLSVAALFGHAVSHQLPA
                     RAAETLWLHALAVAHAAQEIAHLKRLPDLDTAYLLGLLHDLGYFLIEYSRAGSLDALA
                     DATVRDHITQEEAEHRLFGADHAELTWRLLEHWELPDELIEPIRVHHHSGIDPQGMAA
                     LILGAEHLARAGETVAALYADLGHPFAPLAVDRLGLETLFDDRLALSAEAVDGLLEGI
                     IDAVASLRECARVLA"
     gene            complement(7903..>8136)
                     /locus_tag="VIS73_12525"
     CDS             complement(7903..>8136)
                     /locus_tag="VIS73_12525"
                     /inference="COORDINATES: protein motif:HMM:NF025701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HEY5764025.1"
                     /translation="VALGQRPTAGYGLELASTRGELRDRQLSIEISRQQPAADSMTAQ
                     VLTSPCLLIEIDAAANRVDAVVVRDPAVGSVAR"
BASE COUNT         1388 a         2707 c         2699 g         1342 t
ORIGIN      
        1 aaaactgcta ccgggacgac ctcactaccg tgcagcgcca ccagcgcatg cgcgggccgc
       61 acgaagttca cggtgctcca tccgtcggca agctgatagc tcatcacctt ggggatcggc
      121 agcgccgcga gcgccgcctc gatcgccttc tgcaagccgt cggcaagcgt cgcgccgggc
      181 gcgacgcttt catagaacag cgcttcggcc ttgcctacgc cttcgcggcg caggccagcg
      241 accaccgatt catcggcacc gagcgccgcc agcttcttga gcagcgccgg cgtcggcttg
      301 ccctgcgcat ccagcccgac cgacaccggc atcagctttt gcacgagcgg cttgtcggcg
      361 gcgacggcga gcacaccggt caggtgcgcg gccattcgcc gcggcgaagc gtaggcggta
      421 accgcggttt cggccgatgc caggcccgac tgtcgcagcg aggccgcgag cgacgctgca
      481 aaggcctcgc cgagatgctt gagcgccttg ggcggcagtt cctcgacgaa cagctcgacg
      541 agcagattcc tggccgtcat ctaggcagcc ctcttttcca gctgcgccag cacttcgtcg
      601 gcccagggtt taggcgccat cgggaagccg agccgcgcgc ggctgtcgag gtagctctgc
      661 gccacgctgc gcgccaagtt gcggatacga ccgatgtagg cggcgcgctc ggtgaccgag
      721 atggcgccgc gcgcgtcgag caggttgaag gtatgcgcgg ccttcagcac ctgttcgtag
      781 gcgggcagcg cgagctgcgc acccatcagg tgctgcgcct gtttctcgtg cgcaccgaag
      841 gctgtgaata ggaactcggc gtcagaatgt tcgaagttat aggccgactg ctcgacctcg
      901 ttctggtgat aaacgtcacc gtatgacagc cggccgttcg ggcccacggt ccaggtcagg
      961 tcgaagacat tttcgacgcc ctgcaggtac atcgccaatc gctcgaggcc gtaggtgatc
     1021 tcgccggtaa tcggcttgca gtcgatgccg ccgacctgct ggaagtaggt gaactgggtg
     1081 acctccatgc cattcatcca gacttcccag cccagccccc aggcgcccag cgtcgggttt
     1141 tcccagtcgt cctcgacgaa acgcacgtcg ttctgcttta gatcgaagcc gagcgcttcg
     1201 agcgagccga gatagagctc aaggatgttt tcgggcgccg gtttcaacac tacctggtac
     1261 tggtagtagt gctgcatgcg gttcgggttc tcgccgtagc ggccgtcctt cggccggcgc
     1321 gagggctgga cgtagccggc gttccacggc tcggggccga tcgcgcggag aaaggtcgcg
     1381 gtgtggctgg tgccggcgcc gacttccatg tcgtagggtt gcagcagcgc gcagccgtgc
     1441 gcgctccagt agttctgcag gcgcaggatc acgtcctgga agctcggttt gtcagttgcc
     1501 atcacggcca agggtcgatt gcggaagggg ctgattctat cagtctgccg cgcgtcccag
     1561 ccgccgcgca cggccgacga tgagcgccac cgcaagcagc agcaggctgg cgatgaggac
     1621 cggccgatcg ccccagcgca gatacggtgt ctcgccggtc ctgccctgca cgtcggcgac
     1681 cagcgcgccg cgcacaaagg gttcgagaac ggcgctaacg cgcccatccg gctcgacgat
     1741 cgcggtcatc ccggtattgg tcgcgcgcag catcggccgg cccgtctcac gggcgcgcat
     1801 ccgggcgatt tgcagatgct gcggctgcgc cagcgagcgg ccgaaccagg ccgtgttgga
     1861 aacgttgacg agaatcgccg ccgcacgggc gcccggccgc agcgcgtcgc cgaagacatc
     1921 ctcgtagcag atgttgacgg cgaccgtcgt gccgccgacc cgcagcggcg gctggtcggc
     1981 acgtcccgcc gaaaagcccg acatcgggat cgacagctgg cgcatcagcg tcgcgaaacc
     2041 cggcggcacg aattcgccga agggcaccag atgcaccttg tcgtagcgct gggtcggcgc
     2101 cgtgccgacg ctgaaaaccg agttggtata gcgctcgccg gtgccggcca ctgcgccgat
     2161 cagcacgtcg ccaccgcgag cccgagcctg ttcccgaagc gactcgacga aggccggcgg
     2221 caacgcctcc cagaacgccg gcagcgcggt ctcgggtagc acggtgagat cggccgggtg
     2281 cgcgtcgaac aaggcgtcat aggtgcgcag tgaggtctgg aagctctccg gccgccattt
     2341 catgtcctgc gcgatgtttc cctggagcag cgcgacacga accggccggc cggccgggtg
     2401 ggtccactcg aggataccga gcacggcgcc gcctgcgccg agcagagccg cgatcaacaa
     2461 tggcaccgct gcatacgccc agcgcgggtg cccgctcgcc gggcagtaac cgctgcgcca
     2521 gcgccagacg gcctcgccga ggcaggccgc gatcaacgcc gtaacgaagg agacgccgta
     2581 ggtaccgaac agcggcgcca gtcccgcgag cggcgaatcg ccgccctgcg agtaaccgac
     2641 ggcaagccag ggaaagccgg tgaacagggt gccgcgcagc cattcgccga gtacccaggc
     2701 ggcggagaag agcggcacgc gcaggcccgc cgtcgataac cgggcaaaca ccgccacggc
     2761 cagtgccggg aacagcgcga ggaccatgca aaacaccagc gtggacagcg ccgcgagcca
     2821 ggcgaccatg ccgccgaaca cgctgagact gacgtagacc caggagacgc cgccgaggaa
     2881 gcagccgaga ccgaacgcga aaccgatcgc cgcggcggcc cgggtaccgg gttggtgcag
     2941 gccaagcagg tagagcaccg ccagagccag cggtgccagc ggataccagg cgagcggcgc
     3001 gaaggcggca accagcagaa cgccggcggc aaaggccgcc agcagctcag tcggtcgcgg
     3061 caggcgccgg agcggcatgg cgcgggcgat cgatcagcag cgtataaagc cggcggctgt
     3121 cggcacgcag gatctgcacg cgcaggctgg cgacctcgat gatctcgttg cgcttcggta
     3181 cgcgcccgaa ttgtttgagc agcaggccgc cgatggtgtc gcattcgggg tcttcgagcg
     3241 aggtgccgaa ggcggcgttg aacgcgtcga tctcggtctg tgccttgacg cggtagcgac
     3301 cggcctggtc gatctggata ttgtcggcgg cctcgtcgaa gtcgtactcg tcctcgatgt
     3361 cgccgacgat ctgttcgagc acatcttcga tcgtcaccag gccggcgacg ccgccgtact
     3421 catcgacgac gacggccatg tggttgcgcg agacgcggaa ctctcgcagc agcacgttga
     3481 ggcgcttgct ctcgggaatg aatacggccg gccgcagcca gtcgcgcaga tcgaagtcgt
     3541 cttcgagttg caggcgcagc agatccttgg ccagcaggat gccgatcacg ttgtccttgc
     3601 tgccctgggt gaccggaaag cgcgagtgcg cgcgctcgag cgcgtaacgg acgatgttgc
     3661 cgatcgactc gtcgacatcg acgacatcca tctgcgcgcg cggcaccatg acgtcgcgga
     3721 cactggtatc ggacgcggcc agcgcgcctt cggtgattga cagcgcatcg gcgtcgagca
     3781 gattgcgctc gtaggccgaa tgcagcaggc gcagcagctg gtctcggtct tccggctctc
     3841 gcagcagcag ggtcgtcaga cgttcgagaa atccggggcg ttcggcagaa ctatccatgg
     3901 cgtgcgtcgc cgcggcgcgg cgaccgtcgt gggtcaatcg gtggcatagg ggtcatcgta
     3961 tccgagggct gccaggatct cgcgttcgcg ggtttccatc gcctcggcat cattgtcatt
     4021 gtcatggtcg tacgcttgca ggtgcagcat accatgcacg atcaggtggg ccagatgcgc
     4081 ttccagggcc ttgccctgcg ccactgcttc gcgcgccgcc acggtcgtgc acagcaccag
     4141 atcgccaaac acgcgcggtt cgctttcgta ggggaaggaa agcacgttgg tcgcgtagtc
     4201 cttgccgcgg tagtcacggt taagcgcgcg cccttcatcc tcgtcgacga aacggatcgt
     4261 tacctgtgcg ccgccggcaa gcgccgcgcg cgcccagcgc cgcaccgcgt cgcgcgcggg
     4321 agccaaggcg cgatcgccgg cgtattgcac gctgaggtcc aggcgtcggg gcggacgcgc
     4381 gccgccggcc tgctcaggtt tcgcggaagg catcgtaggc ctggacgatg cgggcgacga
     4441 gcgggtgacg aaccacatcc tcggcgccga actcgatgat gccaatgccg cgcactgctg
     4501 acagcacatg gcgcgcctcg atcaggccgc tctgctggcc gcgcggcagg tcgacctgcg
     4561 taacgtcacc ggtgacaacc gctttggcac cgatgccgat gcgggtaagg aacatcttca
     4621 tctgctcggg cgtggtgttc tgcgcctcgt cgagaatgat gaaggaatga ttgagggtgc
     4681 ggccgcgcat gtaggcaagc ggcgcaattt cgatcgcacc cttctcgaac agcttggcca
     4741 cgcggtcaaa acccatcagg tcgtagagcg cgtcgtagag gggccgcaga tagggatcga
     4801 ccttctgcgc cagatcgccg ggcaggaaac cgagccgctc gccggcctcg acggcgggcc
     4861 gcgtcagcac gatgcgctcg acgtgttcgc gctccagggc atcgacggcg caggcgactg
     4921 cgaggtacgt cttgccggtg ccggcaggcc caatggcgaa ggtgatgtcg tgggcggcga
     4981 ccgccttgag atagtcgatc tggcgcggcg tacggccgtg cagctcggtc ttgcgggtca
     5041 tcagctgcac ctggtcggga ccggcagcgg cacgggcaag tggcgcattc gccaattcga
     5101 tgaggccaag ctggatgtcg tctaccgtca gtccgccatc ggcgctggca tagaaggttt
     5161 ggagcgcctc ggctgccttc gccgtctgcg ccgaatcgcc gtgcagcgtg aagcgctccc
     5221 cgcgccggct gatcgatacg tcgagcgccg attcaatctg ccgcaggtgc tcgtcgagcg
     5281 ccccgcagag attcgccagc cgcgcgttgt cgaccggcgc gagcacgatc tcgcgcggac
     5341 gactgcgtct tgcctgctgc gggttagcca ttggcgacgg cttcgcgggt cagcacctcg
     5401 ccgcgcagcg agtgcggcag cgccgcggta acggtcaccg gcacgaaatg gccgatcagc
     5461 cttgggttgc cggcgaagtt cacgattcgg ttgttgtcgg tgcggccggc cagctcttcg
     5521 gcgtgcttct tcgagacgcc ttcgaccagc acccgctgga tgctgccgac catcccttgg
     5581 ctgaccgttg cggcgagttc gtcgatgcgc ttctgcaggc gcgccagccg cgccagcttg
     5641 accgcctcgg cggtgctgtc ggccatatcg gcggccggcg tccccgggcg cggactgaag
     5701 acaaagctga aggaggcgtc gaagccaatc tcgtcgatca gcttcatcgt cgcctcaaat
     5761 tcggcctcgg tttcgccggg aaagccgacg atgaaatcgg tcgacagcga aaggtcgggc
     5821 cgcacggcac gcagcttgcg gacgacgctc ttgtattcga ggatcgtgta accgcgcttc
     5881 atcgccgcca gtacgcgatc cgaacccgac tgcaccggca ggtgcagatg cgagacgagc
     5941 ttcggaactc gggcgaaggt ttcgatcagc cgcgcattca tttcgcatgg atgcgacgta
     6001 gtgaagcgaa tgcgctcaac cgccggcatc tctgccagca attcgatcag cagtgccaag
     6061 tcggcgggct cgccgtcgtc catctcgccg cgataggcat tgacgttctg cccgagcagc
     6121 gtgatctcgc gcacgccttg cacggtcagc cgctcgacct cggcgagcac gtcggcgagc
     6181 ggccgcgaga cctcatcgcc gcgcgtaaag ggaacgatgc agtaagtaca gaacttcgaa
     6241 cagccctcca tcaccgagac gaaggcggtg ccgccctcga ctgtcggcgc cggcatggcg
     6301 tcgaacttct cgatctcggg gaatgacaca tcgaccgccg cccggccttc gctgcggcgc
     6361 tgggcgatca gttgcggcag ccgatgcagc gtctgcggcc cgaacaccac gtcgacgtag
     6421 ggcgcgcgac gaacgatcgc ctcgccttcc tggctggcaa cgcagccgcc gacgccgatc
     6481 agcaggtcgg gcttctgcag cttgagcagg cgcgcgcggc caagctcgtg gaagaccttc
     6541 tcctgcgcct tctcacggat cgagcaggtg ttgaaaacga tgatatcggc atcgtctggg
     6601 cgatcggtct tggcgacgcc ctctccggct gccagcacgt cggcgatgcg atccgagtcg
     6661 tactcgttca tctggcaacc gaaggttttg atgtacagct tcttcacgaa agaaaaaatc
     6721 cttgtacggc aggggcttgc attctagcag ggggccggcg cgtcttgacc gaggaaaggg
     6781 cgggccgtaa aatcccggcc ctcggtttgg tgatgtagct cagacggtta gagcgatgga
     6841 ttcataaccc ataggtcggc ggttcgattc cgcccatcac caccactcac tccctcgggc
     6901 cgatcctcac ggatgcggcc cgttgtgttt tctgccccga cctatactgg attgacaagc
     6961 acacgcggga ggggcgagcg atgaccgcag ccatccttca catcgatctc gacgcgctgc
     7021 cacctctctc gccgctggca gggaagttgc tgcgcctcga ctggaacgac gaagcgagtg
     7081 accggcacct cgtcgaaatc atccgcagcg agccgcagct gagcgcgcgc atcgtcggac
     7141 tggcgaattc ggtcgccttc gccgcccccg gcggcagacc ggtgatcgcg gtcgaggcct
     7201 gcctgcagcg catcggcgtg cgccgcgccg cgctgctctc ggtggcggcg ctgttcggcc
     7261 acgcggtctc gcaccagttg ccagcgagag ccgccgagac gctttggctg cacgcgctgg
     7321 ccgtcgccca tgccgcgcag gagatcgcgc acctgaaacg cctgcccgac ctcgacaccg
     7381 cctatctgct tggtctgctg cacgacctcg gctatttcct gattgagtat tcgcgcgccg
     7441 gatcgctcga tgcgctggcg gatgccaccg tccgcgacca catcacgcag gaggaagccg
     7501 agcatcgtct gttcggtgcc gaccacgccg aactgacgtg gcgcctgctt gaacactggg
     7561 aactccccga tgagctgatc gagccgatcc gcgtccatca tcattcgggc atagacccgc
     7621 aggggatggc ggcgctgatc ctcggtgccg aacacctcgc acgcgccggg gaaaccgtcg
     7681 ccgcgctcta cgccgacctg ggccaccctt ttgcaccgct ggcagtcgac cggctcggcc
     7741 ttgaaacgct gttcgatgac cggctcgcgc tatcggcaga ggccgtcgac ggccttctcg
     7801 aaggaatcat cgacgccgta gcgagtcttc gcgaatgcgc ccgggtcctc gcttgatccc
     7861 gtccgcgcgc ccaatctcgc tccccgaggt gagtcgcggc agctagcgcg ccaccgaccc
     7921 gacagctggg tcgcgcacga cgacagcgtc cactcggttc gccgcggcgt cgatttcgat
     7981 caacagacac ggtgaagtca gcacctgcgc cgtcatcgaa tctgctgccg gctgctgccg
     8041 gctgatctcg atcgacagct gccggtcgcg caactcgcca cgggtcgatg ccagttcgag
     8101 gccgtatcca gccgtcggtc gctgacccag cgccac
//
LOCUS       DATDAA010000421         8467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_345193,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000421 DATDAA010000000
VERSION     DATDAA010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8467
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345193"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..168)
                     /locus_tag="VIS73_12530"
                     /pseudo
     CDS             complement(40..168)
                     /locus_tag="VIS73_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
     gene            125..448
                     /locus_tag="VIS73_12535"
     CDS             125..448
                     /locus_tag="VIS73_12535"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEY5764026.1"
                     /translation="MPSITSSRLSSVAPLLDSPVGLDGFTVCSTVLVAGSDIEAETLA
                     ACRAISPREPSTRVGITALRKVLVARYLGHSSESARAWFIDLWRILRPAVTGREVAIP
                     RIWHT"
     gene            459..761
                     /gene="ureA"
                     /locus_tag="VIS73_12540"
     CDS             459..761
                     /gene="ureA"
                     /locus_tag="VIS73_12540"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEY5764027.1"
                     /translation="MELTPREKDKLLIFTAALLAERRKARGLKLNYPEAVAFITAGIL
                     EGARDGCSVAELMSSGATLLSRDEVMDGVAEMIPEIQVEATFPDGTKLVTVHNPIV"
     gene            771..1076
                     /locus_tag="VIS73_12545"
     CDS             771..1076
                     /locus_tag="VIS73_12545"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEY5764028.1"
                     /translation="MIPGEMQIEPGEIELNAVRRTVTVTVANTGDRPVQVGSHYHFFE
                     ANDALSFDRAAARGMRLNIAAGTAVRFEPGQARTVELVDYAGARVVYGFQGKVMGKL"
     gene            1192..2892
                     /gene="ureC"
                     /locus_tag="VIS73_12550"
     CDS             1192..2892
                     /gene="ureC"
                     /locus_tag="VIS73_12550"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEY5764029.1"
                     /translation="MKISRQAYAEMFGPTVGDRVRLADTELWIEVEKDYTIYGEEVKF
                     GGGKVIRDGMGQSQRVSSEVADTVVTNALIVDAVAGVIKADIGLKGGQIQAIGKAGNP
                     DVQPGVTIVIGPGTEIIAGEGMIVTAGGIDSHIHFICPQQIDEALMSGVTTMLGGGTG
                     PATGTYATTCTPGPWHIHSMLAAADAFPMNLGFLGKGNASRPEALVEQVEAGAMGLKL
                     HEDWGTTPAAIDNCLSVAEKADVQVAIHTDTLNESGFVEATIAAFKGRTIHTFHTEGA
                     GGGHAPDIIKAASLPNVLPSSTNPTMPYTVNTIDEHLDMLMVCHHLDASIAEDVAFAE
                     SRIRRETIAAEDILHDLGAFSMMSSDSQAMGRVGEVIIRTWQTAHKMKVQRGALKEDT
                     ARNDNQRVLRYIAKYTINPALTHGIAHVVGSIAPGKLADLVLWKPAFFGVKPSLIVKG
                     GMIAAAAMGDPNASIPTPQPVHYRPMFGSFGGGLRTALTFVSQAALNNPAVAALGLAK
                     RLEPVAGCRTVKKADMVRNSATPAIDVDSETYAVRADGELLVCEPAAELPLAQRYFLF
                     "
     gene            2895..3419
                     /gene="ureE"
                     /locus_tag="VIS73_12555"
     CDS             2895..3419
                     /gene="ureE"
                     /locus_tag="VIS73_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HEY5764030.1"
                     /translation="MLLIERFAEGGGDAAEVLELPFDARCRSRLRTRVVSGEECGLFL
                     ERGTVLRGGDRLLANDGRIVAVAAAAESLMEASTDDRAGAALLIARAAYHLGNRHVAV
                     QLMPGRLRFVADHVLGDMVRGLGLSLIALSAPFEPEGGAYGSHAHGGHAHPHGHSSEG
                     EGRGPRIHDGFTYK"
     gene            3416..4093
                     /locus_tag="VIS73_12560"
     CDS             3416..4093
                     /locus_tag="VIS73_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HEY5764031.1"
                     /translation="MIPALPRLLQLASPALPVGAYTYSQGLEWAVEVGTVHDEASAQR
                     WIGHLLAHNLGCYELPTLGRLMAAWEVGDIAAVRALDARYLASRETAELRAESLQMGH
                     SLRRLLGELGGVPASLIDRVGEIEQPAFITIWSGVATTWKIAPPDALAAWVWSWAENQ
                     TMAAIKSVPLGQAAGQRTLLAVGDRIGALVAAALTQDETDDSNFAPGFSIACACHETQ
                     YSRLFRS"
     gene            4090..4734
                     /gene="ureG"
                     /locus_tag="VIS73_12565"
     CDS             4090..4734
                     /gene="ureG"
                     /locus_tag="VIS73_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEY5764032.1"
                     /translation="MNQSVKKDAAGGRAAALRVGIGGPVGSGKTALTLALCRALRDRY
                     EIAAVTNDIYTAEDAEFLVRHAALAPERIIGVETGGCPHTAIREDASINLEAVDRLNA
                     RFSGLQIVFIESGGDNLAATFSPELSDLTIYVIDVAAGDKIPRKGGPGITKSDLLVIN
                     KIDLAPLVGASLEVMDRDARRMRGERPFLFSNMKSGKGLSEIIAFIEKQGLIGV"
     gene            complement(4750..5517)
                     /locus_tag="VIS73_12570"
     CDS             complement(4750..5517)
                     /locus_tag="VIS73_12570"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEY5764033.1"
                     /translation="MQGNEQIADTVAALVREARRILVITGAGISADSGLPTYRGTGGL
                     YNGRLTKSGMSIEEVLSGTTFARAPQLTWEYLLEIERACRGAQPNPAHHAIAELGETR
                     QVTVLTQNIDGLHRVAGTRDLIEIHGTLYRLLCLGCGARSEVPDYAELDLPPTCRACG
                     ELLRPEVVLFGEALPRECLHALHNALLDGPDLIVSIGTSSVFPYIAEPIFYGRENGIA
                     TVEINPEETQISGAVDHIWRAGAAEAMVAVMDQLADE"
     gene            complement(5558..5992)
                     /locus_tag="VIS73_12575"
     CDS             complement(5558..5992)
                     /locus_tag="VIS73_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5764034.1"
                     /translation="MPQRPISELIAKQTIVTADESLPVTKAATLMRDAKVGALLVVKG
                     SKLAGIFTERDALYRVIAAGLDPAKTRLSEVMTSNPESIPANRPFGHALHLMHERGFR
                     HVPVVDHGRPVGMVSARDALGLELKAFEDELQDRDHITEILG"
     gene            6226..6720
                     /gene="nikR"
                     /locus_tag="VIS73_12580"
     CDS             6226..6720
                     /gene="nikR"
                     /locus_tag="VIS73_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HEY5764035.1"
                     /translation="MERITISLEAELAETFDQWLAARGYQNRSEAVRDLIRAQIAESR
                     NEAPGERHCVANLSYVYNHHERDLAERLMALQHDHHDLSVATMHAHLDHEHCLESAIL
                     KGPAKAVQRFAAALVAERGVQHGQLNLVTVDVEKGAHSHGGGKPHSHSHGKGGTHIHY
                     RPKP"
     gene            6890..7456
                     /locus_tag="VIS73_12585"
     CDS             6890..7456
                     /locus_tag="VIS73_12585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY5764036.1"
                     /translation="MKAKLRSDRLVYTGFFRLRQLTVDVPRYDGLVQDSVVREVVERS
                     DVAAAILVDPDRDAVVLVEQFRAGPFAAGDDGWLIDLVAGRIESGDTPEETIRREVAE
                     EAGVACADLCRLGHFYTAPHISSERVWLFAASVDSTGVSGTHGLAHEGEDIRPVVLPR
                     AKAVELMEASTLSLWAGVALRWLAAQPR"
     gene            complement(7453..>8467)
                     /locus_tag="VIS73_12590"
     CDS             complement(7453..>8467)
                     /locus_tag="VIS73_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEY5764037.1"
                     /translation="VPDAAGEAGARELLKAVSGLNADDLVLCLISGGGSALLPAPLPG
                     LTLADKQSITKSLLACGAPIDEINCVRKHLSAIKGGRLAAACAPARVVTLIISDVPGD
                     DPAAIASGPTVPDPSTVADARAVFTRYRLAIPPQLAAVLDGAAADTPKPGDPRFAGHE
                     TKIIASNQVSLEAAAAQVRQAGLTPVILGDGIEGESRDVARVHAAIARQITLHGQPVA
                     APCVLLSGGETTVTLRGNGRGGRNAEFNLALAIALKATSGIWSLAADTDGIDGVEDNA
                     GAFVGPDTLARALAAGRIAQADLDRNDAHGFFSATGDLLVTGPTRTNVNDFRAILVAP
                     PRD"
BASE COUNT         1373 a         2705 c         2949 g         1440 t
ORIGIN      
        1 gggctcgatc tgcatttcgc ctggaatcat cacggcctcc tagacgatcg ggttatggac
       61 agtcaccagc ttggtgccgt cgggaaaagt cgcctcgacc tggatctcgg ggatcatctc
      121 ggcgatgccg tccattacct cgtcgcggct cagcagcgtg gcgccgctgc tcgactcgcc
      181 ggttggcctc gacggtttta cggtgtgcag tacggtgctc gtcgccggca gcgacatcga
      241 ggccgagacc cttgccgcct gccgcgcgat atcgccgcgc gagccgtcga cccgagtcgg
      301 catcactgcg ctgcgcaagg tgttggtggc gcgctatctc ggccattcat ccgagagcgc
      361 tcgcgcctgg tttatcgatc tttggcgcat cctgcggccg gccgtgaccg gccgcgaggt
      421 cgctattccc cgcatctggc atacctgaag gaggagccgt ggaacttacc ccgcgcgaaa
      481 aggacaagtt gctgatcttc accgccgcgc tgcttgccga gcggcgcaag gcccgcggcc
      541 tgaagctgaa ctacccggag gcggtcgcct tcatcaccgc cggcatcctc gaaggcgcgc
      601 gcgacggatg cagcgtcgcc gagctgatga gcagcggcgc cacgctgctg agccgcgacg
      661 aggtaatgga cggcgtcgcc gagatgatcc ccgagatcca ggtcgaggcg acctttcccg
      721 acggcaccaa gctggtgact gtccataacc cgatcgtcta ggaggccgtg atgattccag
      781 gcgaaatgca gatcgagccc ggtgagattg aactgaatgc cgtgcgccgc acggttacgg
      841 tgacggtggc caacacgggg gatcgtccgg tacaggtcgg ctcccactat cactttttcg
      901 aggccaacga cgcgctctcc tttgatcgtg ccgcggcgcg cggcatgcgc ctcaacatcg
      961 cggccggaac cgcggttcgc tttgagccgg ggcaggcgcg caccgtcgaa ctggtcgact
     1021 atgccggtgc gcgggtggtc tacggttttc agggcaaggt gatgggaaag ctatgacgac
     1081 cttttcccca acccccttcc cgagggaggg ggtgaatgcg gttcgcgggc tgtgcccgct
     1141 cggcgtcatt gaatggtcac ctattcgggg cggcccagcg gaggtgacga aatgaagata
     1201 tcccgacagg cctatgccga gatgttcggc cccaccgtcg gcgaccgtgt gcggctcgcc
     1261 gataccgaac tgtggatcga ggtggagaag gactacacga tctacggcga ggaagtaaag
     1321 tttggcggtg gcaaggtcat ccgcgacggc atggggcaaa gccagcgtgt ttcctccgag
     1381 gttgccgata ccgtcgtcac caacgcgctg atcgtcgatg cggtagcggg cgtcatcaag
     1441 gccgacatcg gtctcaaggg cggccaaatc caggccatcg gcaaggccgg caaccccgat
     1501 gtccagcccg gcgtgacgat cgtgatcgga ccgggaaccg aaatcattgc tggcgagggg
     1561 atgatcgtca ccgctggggg catcgacagc cacatccatt tcatctgccc gcagcagatc
     1621 gacgaggcgc tgatgtcggg cgtcacgacc atgctcggcg gcggaaccgg acctgcgacc
     1681 ggcacctatg caaccacctg cacgccgggc ccctggcaca tccactcgat gcttgcagcc
     1741 gcggacgcat tcccgatgaa cctcggcttc ctaggcaagg gcaacgccag ccggcccgag
     1801 gcgctggtcg agcaggtcga ggccggcgca atgggcctca agctgcacga ggactggggc
     1861 acgacaccgg cggcgatcga caactgcctg tccgtggccg aaaaagccga tgtgcaggtt
     1921 gctatccaca ccgacacgct caacgaatcg ggcttcgtcg aggcgaccat cgctgccttc
     1981 aaggggagga cgatccatac cttccatacc gagggtgccg gcggtggaca cgcgccggac
     2041 atcatcaagg ccgcgtcgct gccgaacgtg ctgccctcgt cgaccaatcc gacgatgccc
     2101 tacacggtga acaccatcga cgagcatctc gacatgctga tggtgtgcca ccacctcgac
     2161 gcctcgatcg ccgaggacgt tgccttcgcc gagagccgga tccgtcggga gacgattgcg
     2221 gccgaggaca tcctccatga cctcggcgcc ttctcgatga tgagttccga ttcgcaggcg
     2281 atgggccggg tcggcgaggt gatcatccgt acttggcaaa ccgcgcacaa gatgaaggtg
     2341 cagcgcggcg cgctcaagga agacacggcg cgcaacgaca accagcgcgt cttgcgctac
     2401 atcgccaaat acacgataaa tccggcgctg acccatggca tcgcccacgt cgtcggctcg
     2461 atcgcaccgg gcaagctcgc cgacctggtg ctctggaagc cggcgttctt cggggtcaag
     2521 ccgagcctga tcgtcaaggg cgggatgatt gccgcggcgg cgatgggcga tcccaatgcc
     2581 tcgattccga cgccgcagcc ggtgcactac cgaccgatgt tcggcagctt tggcggcggc
     2641 ctccggaccg cgctgacctt cgtttcgcag gcggcgctga acaatcccgc ggtcgcagcg
     2701 ctcgggctgg ccaaacggct ggagccggtg gccggctgcc gcacagtgaa gaaggccgac
     2761 atggtccgca attcggcaac gccggcaatc gacgtcgact ccgaaaccta cgcggtgcgc
     2821 gccgacggcg aactgctggt ttgcgagccg gctgccgagt tgccgctggc gcagcgctac
     2881 ttcctgttct gagcatgctg ctcatcgaac gctttgccga gggcggtggc gatgccgccg
     2941 aggtgctgga attgcctttt gacgcgcgtt gccgcagtcg ccttcgaacc cgcgtcgtca
     3001 gtggcgagga atgtggcctg ttcctcgaac gcggcacggt gttgcgcggc ggcgatcgcc
     3061 tgcttgccaa cgacggccgg atcgtcgccg tcgccgccgc cgccgagtcg ctgatggaag
     3121 cgagtaccga tgatcgggcc ggagcggcgc tactgattgc ccgcgccgcg tatcacctcg
     3181 gcaaccgtca cgtcgccgta caactcatgc ccggccggct gcgcttcgtc gctgaccacg
     3241 ttttagggga catggtgcgc ggtttgggtc tctcgctgat cgcgctcagt gcgcccttcg
     3301 agccggaggg gggcgcctac ggcagccatg cgcacggtgg gcacgcgcat ccgcatggcc
     3361 acagcagcga aggcgaaggg cgtggcccgc gcattcacga cggctttacc tacaaatgat
     3421 tcccgcgctg ccaaggcttc tgcagctggc gagtccggca ttgccggtcg gtgcctatac
     3481 ctactcgcag ggactcgagt gggcagtcga ggtcggaacc gttcacgacg aggcaagcgc
     3541 gcagcgctgg atcggccacc tgctggcgca taacctcggc tgctacgagt tgcccacgct
     3601 cggtcgtctc atggccgcct gggaggtggg tgacatcgca gccgtgcggg cgctcgacgc
     3661 ccgctacttg gccagccgcg agaccgccga gctgcgcgcc gaatcgctgc aaatggggca
     3721 ttccttgcgt cgcctgctcg gcgagctggg tggtgtgccg gccagtctga tcgaccgggt
     3781 tggcgaaatc gaacagccgg ccttcatcac catctggagc ggcgtggcga ctacctggaa
     3841 gatcgctccg ccggacgcgc tggcggcatg ggtctggagt tgggccgaga accagacgat
     3901 ggccgcgatc aagtccgtgc cgctgggtca ggcggctggg cagcgcacgc tgctcgcggt
     3961 cggagatcgc atcggcgcgc tggtcgccgc ggcgctgacg caggacgaga ccgacgattc
     4021 caactttgcg ccgggcttct cgatcgcctg tgcctgtcac gaaacccagt actcaaggct
     4081 atttcgctca tgaaccaatc agtcaaaaag gatgcggcgg gtggacgggc cgcggcactg
     4141 cgggtcggca tcggcggccc agtcggttcc ggcaagaccg cgctgacgct ggcgctgtgc
     4201 cgggccttgc gcgaccgcta cgagatcgcg gcggtgacca acgacatcta caccgccgag
     4261 gacgccgagt ttctggtccg tcacgcggcg ctggcgccgg agcgaatcat cggcgtcgaa
     4321 acggggggct gcccgcacac ggcaatccgc gaggatgcct cgatcaacct cgaagcagtt
     4381 gatcgactca acgcgcgctt ttccgggttg cagatcgtct tcatcgaatc cggtggcgac
     4441 aacctggccg ccaccttcag cccggagttg tccgacctga cgatctacgt catcgacgtc
     4501 gccgccggcg acaagattcc gcgcaagggc gggccgggca ttactaagtc cgacctgttg
     4561 gtcatcaaca agattgacct agcgccgctg gttggcgcct cgctcgaggt gatggaccgc
     4621 gacgcccggc ggatgcgcgg ggagcgcccc ttcctgttct ccaacatgaa gagcggaaag
     4681 ggtctgtcgg agatcatcgc cttcatcgaa aaacagggcc tgatcggtgt ctgaagtcgc
     4741 cgcgattcct cactcgtcgg ccagttggtc cattaccgcg accatcgcct ctgccgcgcc
     4801 ggcccgccag atgtggtcga cagcgccgct gatctgcgtt tcctcgggat tgatctcgac
     4861 cgtagcgatg ccattttcgc ggccgtagaa gataggctcg gcgatgtagg ggaagacgct
     4921 gctggtgccg atcgaaacga tcaggtcggg gccatcgagc agggcgttat gcagcgcgtg
     4981 gaggcattcg cgcggcagag cctcgccaaa cagcacgacc tcggggcgca gcagttcgcc
     5041 gcaggcgcgg caggtgggcg gcaggtcgag ttcggcgtag tcgggcactt cgctgcgcgc
     5101 gccgcagcca aggcagagca ggcggtagag cgtgccgtgg atctcgatca ggtcgcgcgt
     5161 gccggcaacg cggtgcaggc cgtcgatatt ctgcgtcagc accgtcacct gacgcgtttc
     5221 gcccagctcg gcaatcgcgt ggtgcgcggg attcggttgc gcgccgcggc aggcgcgctc
     5281 gatctccaac aggtactccc aggtcagctg cggcgcgcgt gcgaaggtcg tgcccgagag
     5341 cacctcctcg atggacatgc cgcttttggt tagccggccg ttgtagagcc ccccggtgcc
     5401 acggtaggtg ggtaggcccg agtcggccga gatgccggcg ccggtgatga cgaggatgcg
     5461 ccgggcctcg cgcaccagcg cggcgaccgt gtcggcgatc tgctcgttgc cctgcatggc
     5521 ggtacgacgt cgaggcggcg gcggacctga ctggcggtca gccgagaatc tcggttatgt
     5581 ggtcgcggtc ctgcagttcg tcctcgaagg ccttgagttc gagaccgagc gcatcgcgcg
     5641 ccgagaccat gccgaccggc cggccgtggt cgaccaccgg tacatggcgg aagccgcgtt
     5701 cgtgcatcag gtgcagtgca tggccgaagg ggcggttggc agggatgctc tccgggttgc
     5761 tagtcatcac ctcggacagc ctcgtcttcg ccggatcgag gccggcggcg atcacgcggt
     5821 agagcgcgtc gcgctcggtg aagatgccgg caagcttgct gcccttcacc accagcaggg
     5881 caccgacctt ggcgtcgcgc atcagtgtgg cggccttggt gaccggcagt gattcgtcgg
     5941 ccgtgacgat ggtctgtttc gcgatcaatt cgctgatggg tctctggggc atagttgtct
     6001 cctgttgtgc gcggtcgaaa acgcgcggcg cctcatcggg tgcctcggcc cgtccatgat
     6061 gcggcagaat cggttttcgt gcagcccggc cggtggctcg cgggcgacgg cctcgcgcgg
     6121 cccggtgata agatcggctg ccgatggcag cggcatgagc tgccccgatc tgccggtgcg
     6181 gcgcggcaag cgcggcggcg ccggcgcctg cgaaagggcg cgcccatgga acgcatcaca
     6241 atctcgctcg aggccgaact ggccgagacc ttcgaccagt ggctcgccgc gcgcggctac
     6301 cagaatcgct cagaggccgt gcgcgacctg atccgcgctc agatcgcgga gtcgcgcaac
     6361 gaggcccccg gcgagcgcca ctgcgtcgca aacctttcct acgtgtacaa ccatcacgaa
     6421 cgcgacctcg ccgagcggct gatggcgctg cagcacgacc accacgactt gagcgtcgcg
     6481 accatgcacg cccacctcga ccacgagcac tgcctcgaat cggcgatcct caagggcccg
     6541 gcgaaggccg tgcaacgctt cgccgcggcg ctggtcgccg agcgcggcgt gcagcacggc
     6601 cagctcaatc tggtgaccgt cgacgttgag aagggcgcgc attcgcacgg cggcggcaag
     6661 ccgcacagcc attcgcacgg caagggtggc acgcacattc actatcgacc gaagccgtga
     6721 ccgatcggcc ggcgatcacg cgcgatgcgc gtcggttcga tagatcctgt ggcgagcccg
     6781 gcggacagga tggcgaccgt ggcggcatgc gcgagggatt tgggtccgcg aaggcggtcg
     6841 agcaggcgag tgcgctccgg ctcttctgtc tctgcttggt tgtggctcaa tgaaggccaa
     6901 gctgcgttcg gatcggttgg tctacacggg cttcttccgc ctgcggcagc tgacggtcga
     6961 tgtgcccagg tacgacggtc tggtgcagga ctcggtggtc cgtgaggtcg tcgagcgcag
     7021 tgatgtcgcc gcagcgatcc tggtcgatcc cgaccgagac gcggtggtgc tggtcgagca
     7081 gtttcgtgcc ggccctttcg cggctggcga cgacggctgg ctgatcgacc tggttgccgg
     7141 gcggatcgaa tccggcgata cgccggagga gacgattcgg cgcgaggtag ccgaggaggc
     7201 cggtgtcgcc tgtgctgatt tgtgtcggct agggcacttc tacaccgcac cgcatatcag
     7261 cagcgagcgg gtctggctgt tcgctgcgtc ggtcgattcg acaggggtgt cgggcaccca
     7321 tggtttggcg cacgagggcg aggacattcg gccggtcgtt ctgccgcgcg ccaaggccgt
     7381 tgagctgatg gaggccagca cgctatcgct gtgggccggt gtcgcgttgc gctggttggc
     7441 tgcccagccg cgttagtcgc gcggtggcgc taccagaatc gccctgaaat cattgacgtt
     7501 ggttcgcgtc ggcccggtga ccaacaggtc cccagtggcc gagaagaagc cgtgggcgtc
     7561 gttgcggtcg aggtcggcct gggcgatgcg gccggcggcg agggcgcggg ccagcgtgtc
     7621 ggggccgacg aaggcgccgg cgttgtcttc gacgccgtcg atgccgtcgg tgtcggcggc
     7681 cagcgaccag attcccgaag tggccttgag ggcgatggcg agtgcgaggt tgaactcggc
     7741 gttgcgtccg ccgcggccgt tgccgcgtag cgtcaccgtc gtctcgccgc ccgagagcag
     7801 cacgcagggc gccgccaccg gctggccgtg aagggtgatc tgccgggcga tggcggcgtg
     7861 gacgcgggcg acgtcgcgcg attcgccctc gatgccgtcg ccgaggatta ccggcgtgag
     7921 gccggcctgg cgtacctgcg cggcggcggc ttcgagcgag acctggttgc tcgcgatgat
     7981 tttcgtttcg tggccggcga agcgtgggtc gccgggcttg ggcgtatcgg cggcggcgcc
     8041 gtcgagtaca gcggcgagct gcggcgggat ggcgagccgg tagcgggtga acacggcgcg
     8101 agcatcggcg acggtgctcg gatcgggaac cgtcgggccc gaggcgatcg ccgccgggtc
     8161 atcgccgggg acgtcagaga tgatcagcgt caccacgcgt gccggcgcgc aggcggcggc
     8221 gagccgaccg cccttgatcg cggaaagatg tttgcgcacg cagttgatct cgtcgatcgg
     8281 cgcaccgcag gcaagcaagg acttggtgat ggattgcttg tcagcgagcg tcagccccgg
     8341 caatggcgcc ggcagcagcg cggagccgcc gcccgagatc aggcagagaa cgaggtcgtc
     8401 ggcgttgagg cccgagacgg ctttgagcag ttcgcgcgcg ccggcttccc cggcggcatc
     8461 gggaacc
//
LOCUS       DATDAA010000422        21597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_64878,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000422 DATDAA010000000
VERSION     DATDAA010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 21597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21597
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64878"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /gene="flgG"
                     /locus_tag="VIS73_12595"
     CDS             complement(<1..546)
                     /gene="flgG"
                     /locus_tag="VIS73_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEY5764038.1"
                     /translation="MIRSLWIARTGLDAQQMNIDVIANNLANVNTNGYKRSRAVFEDL
                     LYQTLRQPGAQSSQQTTIPSGLQLGTGVRPIAAEKIHTQGNLQRTENALDLAINGPGF
                     FQVLLPDGTTAYTRDGALHKDAQGQLVTASGYALQPAITIPDDAVTVTVGADGTVSVL
                     QAGTATPTQVGTIKLASFVNPS"
     gene            complement(565..1308)
                     /locus_tag="VIS73_12600"
     CDS             complement(565..1308)
                     /locus_tag="VIS73_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HEY5764039.1"
                     /translation="MDRVIYTAMTGAKHAFSQQAALSHNLANATTTGYRAQEHRFRAV
                     EVQGEGLPTRAFAVDASVKDVFDQGTMAFTGRALDAAIHGKGWFAVQGSDGREGYTRS
                     GNFTISPNGELQTTDGRNVLGDGGPLAIPPDSRVTIAGDGTVSVTNETGAQNVVNVVG
                     RLKLVNPPEASLIRGADGLFRTASGVPAPADDTVRLSPETLEGSNVNVVDAMVGMISL
                     ARQFEMQMRLVQSAEANERSAGQLLAPAR"
     gene            complement(1319..2632)
                     /gene="flgE"
                     /locus_tag="VIS73_12605"
     CDS             complement(1319..2632)
                     /gene="flgE"
                     /locus_tag="VIS73_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEY5764040.1"
                     /translation="MAFQQGLSGLNVSAKAIDVISNNIANAATVGFKSASAQFADVYA
                     ASLAGGGASAVGLGVSLAAVAQQFTQGNLTTTNNPLDLAINGSGFFRLSQGGTVTYSR
                     NGQFQTDKNGFLTNSQGARLTGYLAGTSGIIVPTSPGDIQIDSSDIAPVATGSSTGLS
                     GFRGVRANINLDARSTVPANAWAPSGADIITIDPTTYNFSTALTIYDSLGNPHTMSFY
                     FLKQPTSGDWEVRANVDGTLEANVTGFPATVNFDAFGAMTTAMPLNVSVDLAGVNANL
                     AAAGQPVPVNSANTPLTFDVDLGTSTQFGSAFGTNRLEQDGFAPGRLVGINISQDGIV
                     QGRYTNGQSRNLAQVALVNFTAPNGLQPLGGNQWAETSDSGPPLIGAPGTSSLGLITA
                     SAVEDSNVDLTAELVNMITAQRNYQANAQSIRTQDQILQTLVNLR"
     gene            complement(2644..3318)
                     /locus_tag="VIS73_12610"
     CDS             complement(2644..3318)
                     /locus_tag="VIS73_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HEY5764041.1"
                     /translation="MATIQPTTGATHEVIASLGNRQTAAAKEADDMHNRFLTLLVSQI
                     KNQDPLNPMDNAQVTSQLAQISTVNGLEQLNKTMEKLVSFYDEGRAMQAASMVGKHVL
                     VGGDGMELGAEGAIGGLELATAADNVRVVVKDANGLVMRTLELGSREPGSHVFGWDGR
                     TDAGATATPGSYRFAVEAMLGGEATASSPLEFGMVNAVVRSNNGFSLDLGELGNRSFQ
                     AVRRII"
     gene            complement(3366..3773)
                     /gene="flgC"
                     /locus_tag="VIS73_12615"
     CDS             complement(3366..3773)
                     /gene="flgC"
                     /locus_tag="VIS73_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEY5764042.1"
                     /translation="MSLFNMFHIAGSALTAQSARLNVVASNLANAESVAGPDGQPYRG
                     KQVVFETFQLPGSNNAQGVRVQQVVEDAAPPRLVYDPRNPAANGEGYVQMPNVSVVEE
                     MINMISASRSYQSNADVMNTARNLLQRTLAIGQ"
     gene            complement(3788..4195)
                     /gene="flgB"
                     /locus_tag="VIS73_12620"
     CDS             complement(3788..4195)
                     /gene="flgB"
                     /locus_tag="VIS73_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEY5764043.1"
                     /translation="MVSKLDNHFASQSAALDLRVRRQQMIATNIANADTPNYKATDID
                     FSRAFADALAGRGSGGVTLARTAPGHLAARQAMPDSHVQYRPEIQSAVDGNTVDMDIE
                     RAAFADNAMRYEAAVTFVNGRLRGLLDAMQPAR"
     gene            4415..5110
                     /gene="flgA"
                     /locus_tag="VIS73_12625"
     CDS             4415..5110
                     /gene="flgA"
                     /locus_tag="VIS73_12625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEY5764044.1"
                     /translation="MILLTLRSFLAGLLLSAALPAQAMDPPPHPAETAIRDYVMTQTR
                     GSGNRIEVAVDPLPQSILQRPCSAYVPALPAGTRLWGRSVVALNCAAPQSWVVYAPVN
                     IGVYGRYLKTARAIASGQALTTSDFVVSEGDLTGLPDGTFTQAGEALGLRTRVGLAAG
                     LPLGRHHVVVPPVVKQGDPVRVVAKGPGFAASSEGRALANAAEGETVRVRMPSGQTVT
                     GVAQKGGIVELPN"
     gene            5211..5495
                     /gene="flgM"
                     /locus_tag="VIS73_12630"
     CDS             5211..5495
                     /gene="flgM"
                     /locus_tag="VIS73_12630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HEY5764045.1"
                     /translation="MKIEGLNTTSPLAGGSKPKASSAPRADAPASAGDSVAIGAAAAL
                     ASGSEAPVDGARVQAIRQAISEGRFQINPEAIADRLIESARELIESQRRA"
     gene            5545..6003
                     /locus_tag="VIS73_12635"
     CDS             5545..6003
                     /locus_tag="VIS73_12635"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HEY5764046.1"
                     /translation="MESLIDLVSRECRLLTEFVALLGDEEQALVAGDTEALAKVTPRK
                     TEIVSRLNQIADLRNRNMRASGLPADTAGLESWRQRDSSSASAADQLITLAGQARERN
                     RVNGQLIALHLQKTQEALAALTGGDARRQTYGRDGQTEPAKTGYRLIDSA"
     gene            complement(5995..6825)
                     /gene="motD"
                     /locus_tag="VIS73_12640"
     CDS             complement(5995..6825)
                     /gene="motD"
                     /locus_tag="VIS73_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HEY5764047.1"
                     /translation="MSRRRAHLGHSGRRQDDHENVERWMVSYADFITLLFAFFVVMYA
                     ISSVNEGKYRVLSESLVSAFRSSAGGLPQSSALAVRVETKAADPGVNPEEQRSAHTHS
                     MRGVADDILQAMAPLVKQGQVSIIETDRGVTVEINDSILFDRGQAQLGTQAAQAMRSV
                     ARVLARSQFPIIIEGHTDNLPISTPQFPSNWELSAVRATTVLREFIAGGVAASRLTAI
                     GYGDQRPAAANDSAIGRARNRRVTIQIEAAIAGLPGDDSAATSAAPVAPAAPALGPSG
                     "
     gene            complement(6938..7678)
                     /locus_tag="VIS73_12645"
     CDS             complement(6938..7678)
                     /locus_tag="VIS73_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HEY5764048.1"
                     /translation="MDRLSIFGLIVGIGALLVGQLLDGGQTSSLAQLPALVIVIGGTL
                     GAVMLQTPSGTFGRGFRMLRWVWRPPQIDYRRMIEQIVSWSHLARREGLLALEAPANA
                     QQDLFVRKGLQLLVDGAEPDRLRDILEVEVSANEEYLRQAARIWESAGGYAPTIGILG
                     AVMGLIHVMENLTDPSRLGAGIATAFVATIYGVGLANLVFLPIGNKLKLQIARRVTYQ
                     EIIVDGLICIANGDNPRLIENRLKGYLT"
     gene            complement(7671..8420)
                     /locus_tag="VIS73_12650"
     CDS             complement(7671..8420)
                     /locus_tag="VIS73_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HEY5764049.1"
                     /translation="MYTAAGQLESDQLVEHFAPLVKRIAHYLVARLPASVHIDDLIQN
                     GMIGLIEAMSRYQTGLGAQFETYATQRIRGAMLDGLREADWLPRSLRRELRRVDALIH
                     QLEQDFGRPPSETELADGLGLSLAEYQKLLLDARGHQVLHMEDMVSDDEDDFLERHFT
                     DDEADPARILEELGMRESLVGAIGDLPEREKLVMALYYDEELNLREIGEVLGVTESRV
                     CQLHSQAIARLRAVVGASEPVVAIKRSRRRG"
     gene            complement(8436..9326)
                     /locus_tag="VIS73_12655"
     CDS             complement(8436..9326)
                     /locus_tag="VIS73_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5764050.1"
                     /translation="MPDPHGDQAAGLRRLFGGQHLRVVTFLAADAGVGKTQAVAGLAA
                     ALAAQGRQVLIVDENEGADCIAASLGSAVRADLLDVISGRCRLRDALIQLRPGLRVLP
                     AARAAGALDQVTAAQQEALFAAVGDLEVAPDVVLVDARHQHPLGFSPFALATSETVVT
                     LTAQPVSITAAYVLIKGVSQQYGRRRFRVLINRARSAGDAEQVYANLARVAGQRELAM
                     LDLAAVIPSDDAMRRAARLSKSVVEAFPESPAATALRQFASELLYWPSEDDPEGFEDL
                     MRQLLHLSQRITTPRAKPLR"
     gene            complement(9319..10629)
                     /gene="flhF"
                     /locus_tag="VIS73_12660"
     CDS             complement(9319..10629)
                     /gene="flhF"
                     /locus_tag="VIS73_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HEY5764051.1"
                     /translation="MNVKKIVASNAREALRQVKELLGEDSVILTNRNVAGGVEITAVS
                     AAEIAAVTVPARANPPPPREPKRETSRYGSDDDEGYRVSLASRRPKATAPAAATRPHV
                     DFSRSPGVAPAAATTETPSAQVVPNAVMEELRALRQMVEQQLAGFAWRDMTEQDPARG
                     ALLRHMLDAGFSPMFARGSANRVPEGLGDRAAIDWAQDALAHELRGAAPSIDLIDQGG
                     VYALVGPTGVGKTTTAAKLAARCVLRYGAGRVALVTTDGYRIGAHEQLRIYGRILGVP
                     VHGVRDGMELKATLRELSGKHMVIIDTMGMSQRDKNVAAQAAMLGDAKVKRLLLLSAT
                     SRSDTLDDVISAYGQQGVAGCIVSKVDEAVGLAGALDAVIRHRLPLHYVSNGQRVPED
                     LHQPNALYLAHRAFRGLPEDSPHRHRNAEPALLMGSLVAGAGHA"
     gene            complement(10626..12710)
                     /gene="flhA"
                     /locus_tag="VIS73_12665"
     CDS             complement(10626..12710)
                     /gene="flhA"
                     /locus_tag="VIS73_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEY5764052.1"
                     /translation="MASQVLGVDFTKLSLRQAAAPVLIVMILAMMVLPLPTFMLDLFF
                     TFNIALSIIVILVALSTVRPLDFSVFPTLLLVTTLLRLSLNVASTRIVLLDGHTGPDA
                     AGKVIEAFGNFLIGGNYTVGLVVFLILVIINFVVITKGAGRVAEVSARFTLDAMPGKQ
                     MAIDADLNAGLIGEEDARRRRAEISREADFYGAMDGASKFVRGDAIAGIIIMLINVVG
                     GLVVGMLQHNLDVGTAAKTYTLLTIGDGLVAQIPALIISVAAGMVVTRVGEPDTDVSQ
                     QFVAQLFSNPRALSVAGGIVGVLGLVPGMPNLVFILLASILFGAAHYLRKESLAAAEV
                     PAVVAPPPTVAEVEASWADVAPVDVLGLEVGYRLIPLVDKAQDGELLRRIRGIRKRFA
                     QEIGFLPPSVHIRDNLELRPTAYRIQLKGVEIGTGEAFPGQLMAINPGRVAGTVPGTA
                     AKDPAFGLQAVWIDPANREQAQAFGYTVVDASTVVATHLNHLLLSHASELLGRQETQQ
                     LLDHLAKEMPKLVEDLVPKTVALSTLQKVLQALLDEGVPIRDMRTIIETIAENAPRME
                     TPEELSAQVRIALGRAITQQLYPGNVELQVMSLDPTLERVLVQAMAGQSGGGAIEPGL
                     ADTLVSETGAAAGRQEALGLPAVLLVPASIRVLLARFLRRAVPQLKVLSHAEVPDNRI
                     IKVTSIVGGKTA"
     gene            complement(12703..13842)
                     /gene="flhB"
                     /locus_tag="VIS73_12670"
     CDS             complement(12703..13842)
                     /gene="flhB"
                     /locus_tag="VIS73_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEY5764053.1"
                     /translation="MAEESDLERTEPATGRRLEQAREEGQVPHSRELSAFLIMISAVG
                     ILWFLGGWMASRIAAGARKSLTIESTLLNGVESLFPRLIDSFADAMIALLPLLGGLML
                     VALISPAFLGAWIFSPKALTPKFSRLNPVNGLGRIVSKQGLVELAKAIVKAIVVGGVA
                     VLVIWNDLDELVLLATMAPEYGLQQLSDLVLLSFLLVAGSMALVVAVDVPFQLWHYHE
                     KLKMTRDEVKREHKEMEGDPQVKGRIRSLQREAARRRMMAEVPKADVIVTNPTHFAVA
                     LAYKPGMAAPKIIAKGRGEIAGKIRELGAKHGVPLVEAPPLARALFRWSEIGEQIPIR
                     LYAAVAEVLAYIFQLNRYREKGGEYPLPPKDIEVPAELDPGAVNG"
     gene            complement(14022..15893)
                     /locus_tag="VIS73_12675"
     CDS             complement(14022..15893)
                     /locus_tag="VIS73_12675"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEY5764054.1"
                     /translation="MSDFGGMDDLLQDFLQEARELLSDVDNKLVDLERAPHDKALLND
                     IFRGFHTIKGGAGFLNATELVTLCHLTENLFDRLRNGELALTPEIMDVIMEATRGVRD
                     MFGELGQRVQPQPAPVAMLNGLRNVLDGQAPAPAQPAAAAGGPDWQAMHAAVTGASAS
                     AGKAPAASASTAVAAAATTTVVAEGKPAAPVYGRRATDRPGATDSAPMGRRADDRGVV
                     VREATIRVDTARLDQVLNLSGEIGLTKNRLTSLRADILQGRSDSETLHALDQAVSQLD
                     LLVSDLQNAVMKTRMQPIGRLFQKYPRIARDLARQLGKDVELVLAGEDTEVDKTMIED
                     LADPLIHLIRNAVDHGVESPEEREAAGKPAKSVVRLEARQEGDHIMLIVADDGQGMSA
                     ERIRAIAVERRLVSEEEANTLDERQSFNLIFLPGFSSKAQISSVSGRGVGMDVVRTNI
                     QKLNGSIEIRSEPGRGSVFLISLPLTLAILPVLLVRLEDQPFALPLSMVREILPIERE
                     TVQEVGGRATLVVRGEILPVIQLSRLLGWPSERYPEFGVLMQAADRSFILGVDSFAGR
                     DDAVIKSLEDFRPRGVAGVTTLANGQIVLILDLKELLADFGQSESEAKGTRKRLEVS"
     gene            complement(15937..16758)
                     /gene="cheZ"
                     /locus_tag="VIS73_12680"
     CDS             complement(15937..16758)
                     /gene="cheZ"
                     /locus_tag="VIS73_12680"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HEY5764055.1"
                     /translation="MPEMTSMGDSDELQALFDSVAASSGTPAPAPAPAISPSGDSDDL
                     QALFDSIAGEAGQPSAAPGAPAAAGAANSSQAFNRVGQMARQLHDMLRGLGYDKLLSK
                     TVEAIPDATDRLAYVATLTEQAACRVLNATDVAMPVQDSLGAAGSGLAARWDRLYANE
                     LSVDEFRALADETRAFLKGQVPESVSATNAQLMEIMMAQDFQDLTGQVIKKVVTLAQD
                     LEKQMMALLIDIIPDERRTEAVSSLLNGPIINPAGRTDVVSNQAQVDDLLDSLGF"
     gene            complement(16761..17150)
                     /gene="cheY"
                     /locus_tag="VIS73_12685"
     CDS             complement(16761..17150)
                     /gene="cheY"
                     /locus_tag="VIS73_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HEY5764056.1"
                     /translation="MPDQKMKFLVVDDFSTMRRIVRNLLKELGFTNVDEAEDGQVALQ
                     KLQAGDVDFVVTDWNMPNMDGLALLQAIRASDALKHLPVLMITAEAKKENIIMAAQAG
                     ASGYIVKPFTGATLSEKLQKIFEKMGV"
     gene            complement(17185..18129)
                     /locus_tag="VIS73_12690"
     CDS             complement(17185..18129)
                     /locus_tag="VIS73_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEY5764057.1"
                     /translation="MATDLVERKNLLESVDARTRLAGSNQMEILLFSLGTRELFGINV
                     FKVREVGRAPRITRTPNMPKGVEGLISLRGNVIPVLALVHFIDLDDPPSELGSSMMVA
                     EYSKRTVGFLVHDVDRIIRVDWERVKAPESVLSNNSGLITAIIELENGQLVSILDVEQ
                     ILANAFGEAIIVDIPPANVSEDVTMFFVDDSVVARKKISEVLDKLRVKHKHATNGMEA
                     WTRLQAIAAHAQQTGRTVHDDIRVILVDAEMPEMDGYVLTKNIKADPRFDGVPVIMHS
                     SLSSEANRAMGKAVGVDGYVAKFDSGILADTLRPLLER"
     gene            complement(18156..19124)
                     /locus_tag="VIS73_12695"
     CDS             complement(18156..19124)
                     /locus_tag="VIS73_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEY5764058.1"
                     /translation="MSELLKSIDARTKLAGTNKLEILLFTLGTDSRNGRRETFGINVF
                     KVREVMRTPPVTAAPEMPDAVEGMVSLRGALVPVVDLAKYACVRTETPRDIMIVTEYN
                     GMTQGFLVESVDTILRLDWSSMRVPPPMLTAELGGLVTAVTELADGRLVMMMDVEKVL
                     AETAQMDDEMIYRALKPLPKSDATVFFADDSAVARKQLERTFEALGLNYMSSINGRAA
                     WDELERVASLAAAQGKPVSALISLILTDVEMPEMDGYMLTKKVKSDPRFAGIPVVMHS
                     SLSGGSNRTLGESVGVDEYVSKFEPQRLSEVVARRLGIGQDIVPAV"
     gene            complement(19270..19842)
                     /locus_tag="VIS73_12700"
     CDS             complement(19270..19842)
                     /locus_tag="VIS73_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HEY5764059.1"
                     /translation="MSRLIFIGASTGGTEALKEVLTAVPRDCPPILVVQHMPPTFTGS
                     FAKRLDGLCAPQVIEAQAGVPVRGGTVYIAPGSAHLRIRSQPGCYVTELDPSGPFNRH
                     MPSVDVLFNSAAAVVGRNVVGVILTGMGKDGAAGLLAIRSAGGRTLGQDEASCVVYGM
                     PREAAMIGAVEEALPLDRIAPRMLALAAGA"
     gene            complement(19839..20666)
                     /locus_tag="VIS73_12705"
     CDS             complement(19839..20666)
                     /locus_tag="VIS73_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEY5764060.1"
                     /translation="MVREFEFLPADFDRVRRLIHQKAGISLSDLKQDMVYSRLARRLR
                     ATNSPSFAAYLDRLERTGGAEWEQFVNSLTTNLTSFFRESHHFPLLADFLKKRGGRPT
                     RIWCCAASTGEEPYSIAMTVFEALGGSAGGTSIIASDIDTGVLAVARAGQYGGDRVER
                     LEAERLRRFFTESPAAAGKSFTVRAELRRIVDFRQINLLDASWSLGGTVDVIFCRNVM
                     IYFDKATQYRVLSSFAKVLAPDGLLFAGHSESFLHAADLFRSLGKTVYQPVGSARRG"
     gene            complement(20735..>21597)
                     /gene="motB"
                     /locus_tag="VIS73_12710"
     CDS             complement(20735..>21597)
                     /gene="motB"
                     /locus_tag="VIS73_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEY5764061.1"
                     /translation="DFVTAMMAFFLLMWLIGSTAKGDFEGIAEYFKTPMKVAFFGGSG
                     SGDASSIIKGGGTDITKTAGQLRRGDVSTRDRYTPQGGKGDDGESREREKEVLEQLKL
                     NIEDLITKTPSLQQFKNQLLLDITSDGLQIQIVDEQNRPMFDTASAELKPYTRQLLTE
                     IGRLLNGVPNRVSLSGHTDSAQFFGGNLGYSNWELSSDRANAARRELVAGGMDEFKVL
                     RVVGLASTVLFDRNDPLSPINRRISIVVLNQQAEQAMFRQDSGGLRLGTGDRVAAEQL
                     APAVAAPGAQ"
BASE COUNT         3655 a         7008 c         7125 g         3809 t
ORIGIN      
        1 cgacggattg acgaagctgg cgagcttaat ggtgccgacc tgcgtcggcg tcgccgtgcc
       61 ggcctgcagg accgagacgg tgccgtccgc gccgacggtc accgtcaccg catcgtcggg
      121 aatggtgatc gccggctgca gcgcgtagcc gctggcggtg accagctggc cctgagcatc
      181 cttgtgcaag gcgccgtcgc gcgtgtaagc ggtggtgcca tcgggcagaa gcacctggaa
      241 gaaaccgggg ccgttgatcg ccaggtcgag cgcgttctcg gtgcgctgca ggttgccctg
      301 ggtgtggatc ttctcggcag cgatcggccg cacgccggta ccgagctgca gccccgaggg
      361 aatcgtcgtc tgttgcgaac tttgcgcgcc gggttgacgc agcgtctggt agagcaggtc
      421 ttcgaagacc gcgcgcgagc gcttgtagcc gttggtatta acgttcgcca ggttgttggc
      481 gatgacgtcg atgttcatct gctgggcatc aagcccggtg cgggcaatcc acagggaacg
      541 gatcatcgtt tcttctccgg tctatcagcg ggccggtgcc agaagctggc cggcgctgcg
      601 ttcgttggct tcggcgctct gcaccaggcg catctgcatc tcaaactgcc gcgcgaggct
      661 gatcatgccc accatcgcgt cgacgacatt gacgttcgat ccttcgagcg tttccggaga
      721 cagccgcacc gtgtcgtcgg ccggcgccgg gacaccgctg gcggtgcgga acaggccatc
      781 ggcgccgcgg atcagcgagg cctcgggcgg attgaccagc ttgaggcggc caacgacgtt
      841 gacgacgttc tgcgcgccgg tctcgttggt caccgacacc gtgccgtcgc cagcaatggt
      901 tacccggctg tcgggcggaa tcgccagcgg cccgccgtcg ccaaggacgt tgcggccgtc
      961 ggtggtctgc agttcgccgt tggggctgat cgtgaagttg ccgctgcgcg tgtatccctc
     1021 gcggccatcg ctgccctgta ccgcgaacca gcccttgccg tggatcgcgg cgtcgagggc
     1081 gcgaccggtg aaggccatcg ttccctggtc gaaaacgtcc ttgaccgagg catcgacggc
     1141 gaaggcacgg gtcggcagtc cttcgccctg cacctcgacg gcacggaagc ggtgctcctg
     1201 cgcccgatag ccggtggtcg tggcgttggc caggttgtgc gacagtgcgg cctgctgcga
     1261 aaatgcgtgc ttcgcgccgg tcatcgccgt gtagatgacg cgatccatgt cctattcctt
     1321 aacggaggtt gaccagggtc tgcaggatct ggtcctgcgt gcggattgac tgggcgttgg
     1381 cttggtagtt gcgctgtgcg gtgatcatgt tcaccagctc ggctgtcagg tcgacgttgg
     1441 agtcttcgac cgccgaggcg gtgatcaggc cgaggctcga ggtgccgggt gcgccgatca
     1501 gcggcggtcc cgaatccgag gtctctgccc actggttgcc gccgagcggc tgcaggccgt
     1561 tgggcgccgt gaagttcacc agcgccacct gcgccaggtt gcgcgactgg ccgttggtgt
     1621 agcgaccctg gacgatgccg tcctgcgaga tgttgatgcc gaccagacgt cccggggcga
     1681 aaccgtcctg ctccaggcgg ttggtgccga aggccgagcc gaactgcgtg ctggtaccga
     1741 gatcgacgtc aaaggtcagc ggcgtattgg ccgagttcac cggcaccggt tggccggcgg
     1801 cggcgaggtt ggcgttgacg ccggcgagat caaccgagac gttgagcggc atcgccgttg
     1861 tcattgcgcc gaaggcatcg aaattgaccg tcgccgggaa gccggtgacg ttggcctcga
     1921 gcgtgccatc gacgttggcg cgcacctccc agtcgcccga ggtcggctgc ttgaggaagt
     1981 agaagctcat cgtgtgcgga ttgccgagcg agtcgtagat cgtcagcgcc gtcgagaagt
     2041 tgtaggtggt cgggtcgatc gtgatgatgt cggcgcccga cggtgcccag gcgttggccg
     2101 gcacggtcga tcgggcgtcg aggtttatgt tggcgcgcac gccgcggaaa cccgagaggc
     2161 ccgtgcttga accggttgcc accggcgcga tgtctgagga gtcgatctgg atgtcgcccg
     2221 gcgaagtcgg gacgataatt ccggaggtgc cggcaaggta gccggtgagg cgggcgccct
     2281 ggctgttggt caagaacccg ttcttgtcgg tctgaaactg gccgttgcgc gagtaggtga
     2341 ccgttccgcc ctggctcagg cggaaaaagc ccgagccgtt gatcgccaga tcgagcgggt
     2401 tgttggtggt ggtcaggttg ccttgggtaa attgctgcgc cactgcagcc agtgaaacgc
     2461 cgaggccgac ggcactggca ccaccgccgg cgagcgaggc ggcgtagacg tcggcaaatt
     2521 gtgccgaggc gctcttgaag ccgaccgttg ccgcgttggc aatgttgttg ctgattacat
     2581 cgatggcttt cgccgagacg ttgagtccgc tcagaccttg ctggaatgcc atgaccgctt
     2641 ctcctagatg attcgccgga cggcttggaa actacgattg ccgagctcgc ccaggtcgag
     2701 actgaatccg ttgttgctgc gaacaaccgc attgaccatg ccaaattcaa gcggtgacga
     2761 ggcagtggct tcgccaccga gcatcgcctc gacggcgaag cggtagctgc cgggagtagc
     2821 ggtcgcgccg gcgtcggtgc ggccatccca gccaaagacg tggctgccgg gctcgcggct
     2881 gccgagttcg agggtacgca tcaccagacc gttggcatcc ttgacgacga cccgcacgtt
     2941 gtcggcggca gtcgccagct caagcccgcc gatcgcccct tcggcgccaa gttccatgcc
     3001 gtcgccgccg accagcacgt gcttgccgac catcgaagcg gcctgcatcg cacgaccctc
     3061 gtcgtagaag gagacgagct tctccatcgt cttgttgagc tgctcgaggc cgttgacggt
     3121 gctgatctgc gccagttgcg aggtgacctg cgcgttgtcc atcggattga gcggatcctg
     3181 gttcttgatc tgcgaaacca gcagcgtcag gaatcggttg tgcatatcgt cggcttcttt
     3241 tgccgccgcc gtttgccggt tgccgagcga ggcgatcacc tcgtgcgtcg cgccggtcgt
     3301 cggttggatg gttgccatgg ctggttctcc ggatggattt caggactgcg tgtcaggact
     3361 gcggcttact ggccgattgc cagcgttcgc tgcaacaggt tcctggcggt gttcatcacg
     3421 tcggcgttgc tctggtacga gcgcgatgcg ctgatcatgt tgatcatttc ctcgaccacg
     3481 ctgacattgg gcatctgcac gtagccttcg ccgttggcag ccggattgcg cgggtcgtaa
     3541 accagtcgcg gcggcgccgc atcctcgacc acctgctgca ctcgaacgcc ctgggcgttg
     3601 ttcgatccgg gcaactggaa agtctcgaag accacctgct tgccgcggta cggctggcca
     3661 tcgggcccgg caacgctctc ggcgttggcg aggttgctgg cgacgacgtt gaggcgggcc
     3721 gactgcgcgg tcagcgcgct gccggcgatg tggaacatat tgaagaggct catcggcttg
     3781 tccttgctca gcgggccggc tgcattgcgt cgagcaggcc gcgcaggcgg ccattgacga
     3841 aggtgactgc cgcctcgtag cgcatcgcat tgtcggcaaa ggcggcgcgt tcaatgtcca
     3901 tgtcgacggt gttgccgtcg acggccgact ggatctccgg ccggtactgc acatggctgt
     3961 cgggcatcgc ctgccgtgcc gccagatgac cgggcgccgt gcgcgcaagg gtgacgccac
     4021 cgctgccgcg accagccagc gcgtcggcaa aggcccggct gaagtcgata tcggtcgcct
     4081 tgtagttggg cgtgtcggcg ttggcgatgt tggtcgcgat catctgctgc cggcgaaccc
     4141 gcaggtcgag tgccgcgctc tgcgatgcga aatgattgtc gagttttgaa accatcggct
     4201 tgcctgttct ggggatcgtc aatgaatcct ctgcgccttg cttagcgagt tctgtgccag
     4261 ccaatgaaat caatcagttg cggccatggc aacgtcgggg cggcaatgct tgccggccgc
     4321 tggcgccggt caccgggcac gcgcttgccg ttcgggtgag accggcaagc agttggcgct
     4381 tgggcctgca ccgccgctga tgcagaatgc gcggatgata ttgctcacgc ttcgatcgtt
     4441 cctcgccggc ctgctgctct ctgcggcctt gccggcacag gcaatggacc cgcccccgca
     4501 cccggcggaa acggcaatcc gcgattacgt aatgacgcag acccgcggca gcggcaaccg
     4561 gatcgaggtt gcggtcgacc cgttgccgca aagcatcctg caacggccct gctcggccta
     4621 cgtgccggcg ctgccggccg gtacgcgcct ctggggccgc agcgtcgtcg cactcaactg
     4681 cgcggcgccg cagtcatggg tcgtctatgc gccggtgaac attggcgtct acggacgcta
     4741 tctgaagacg gcaagggcga ttgccagcgg gcaagcactc accaccagcg acttcgtcgt
     4801 aagcgagggc gacctgaccg gtctgcccga cgggactttc acccaggccg gcgaggcgct
     4861 gggcctgcgc acccgcgtcg gcctcgccgc tggcctgccc ctgggccgcc accacgtcgt
     4921 cgtgccgccg gtagtcaagc agggcgaccc ggtgcgggtg gtcgccaagg gacccggctt
     4981 tgccgcgagc agcgagggcc gggcgctggc caacgccgcc gagggcgaaa ccgttcgggt
     5041 tcgcatgccc tcggggcaga cggtgaccgg cgtggcgcag aagggcggca tcgtcgaatt
     5101 gcccaactga attcggttgc tggcgcggga agaccgaatt ccgggtgtcg cgaactcaag
     5161 aattgcggca aactgccgtt agcgtgtttg accacacatt cgggactgcc atgaagatcg
     5221 aaggccttaa caccacctcg ccacttgccg gcggcagtaa gcccaaagca agctcggctc
     5281 cccgcgccga cgccccggcc agcgccggcg actcggtcgc catcggtgcc gcagcggcac
     5341 tggcatccgg aagcgaggcg ccggttgacg gcgcacgcgt ccaggcgatc cggcaggcaa
     5401 tcagcgaagg ccggttccag atcaaccctg aagcgatcgc cgaccgcctc atcgagtcgg
     5461 cacgcgagtt gatcgaatca cagcggcgcg cctgattcgg cagccgcccg agacagcggc
     5521 cagccggccg gcactagaca tccggtggaa tccctgatcg atctcgtcag ccgcgaatgc
     5581 cggttgctga ccgaattcgt agcgctgctc ggcgacgagg agcaggcgct ggtcgccggc
     5641 gacaccgagg cactggcgaa ggtcacccca cgcaagactg aaatcgtctc gcgcctcaac
     5701 cagatcgccg acctgcgcaa tcgcaatatg cgcgccagcg gcctgcctgc cgacacggcc
     5761 gggctggaaa gctggcgcca gcgcgacagc agcagcgcca gcgccgccga tcagcttata
     5821 acgctggccg ggcaggcacg cgaacgcaat cgcgtcaacg gccagttgat cgcactgcat
     5881 ctgcagaaaa cccaggaagc ccttgccgcc ctcaccggcg gcgatgcccg gcggcagacc
     5941 tacggtcgtg acgggcagac cgagccggcg aaaaccggct accggctgat cgactcagcc
     6001 tgaggggccg agagcagggg cagcgggcgc cacgggcgcc gccgaggtgg ccgctgagtc
     6061 atcgccgggc aggccggcga ttgcggcttc gatctgaatc gttactcgcc ggttgcgcgc
     6121 ccggccgatc gccgaatcgt tggctgcagc cggccgctga tcaccgtaac cgatcgcagt
     6181 cagccgcgag gcagcgaccc cgccggcgat gaactcgcgc agcaccgttg tcgcccgaac
     6241 cgccgacagt tcccagttgg atgggaactg cggcgtgcta atcggcaggt tgtcggtatg
     6301 cccttcgatg atgatcggga actgcgagcg cgccagaacc cgcgccaccg atcgcatggc
     6361 ctgcgccgcc tgcgttccga gctgggcctg gccgcgatcg aaaaggatgc tgtcgttgat
     6421 ctcgacggtg acaccgcggt cggtctcgat gatcgacacc tgcccctgct tcaccagcgg
     6481 cgccatcgcc tgcaggatgt catcggcgac gccgcgcatg ctgtgcgtgt gcgccgagcg
     6541 ctgctcctcg ggattgacac ccgggtccgc cgccttcgtt tcgacgcgta cagccaaagc
     6601 cgagctctgc ggaaggccac cagccgatga tcggaaggcc gataccaacg attcgctaag
     6661 cacacggtac ttgccctcgt tgacggagga gatcgcgtac atcactacga agaaggcaaa
     6721 cagcagcgtg atgaagtcgg cgtaagaaac catccagcgc tcgacattct cgtggtcgtc
     6781 ctgccgccgc cccgagtgac cgagatgcgc gcggcggcgg ctcacgtcag ataccccttc
     6841 agccggttct cgatgcgcct tgggctctgg cgtaaccctc tgccgcgggt tagcttgcac
     6901 cgcaatctcc tcgtcgttcc gcccccctcg tgtcccgtca tgtcaggtaa cccttgaggc
     6961 gattttcgat caaccgagga ttgtctccat tggcgatgca gatcaggccg tcgacgatga
     7021 tttcctgata ggtgacgcgg cgggcgatct gcagcttgag cttgttgccg atcggcagaa
     7081 agaccaggtt tgcgaggcca acgccgtaga tggtggcgac gaaggcggtg gcaattccgg
     7141 ccccaaggcg cgaaggatcg gtcaggtttt ccatgacgtg gatcaggccc atgaccgcgc
     7201 ctagaatgcc gatggtcggc gcgtagccgc cggccgactc ccagatccgc gccgcctgcc
     7261 gcaggtattc ctcgttggcc gagacctcga cctcgaggat gtcgcgcagg cggtctggct
     7321 ctgcgccgtc gaccaatagt tgcaggccct tacggacaaa caggtcctgc tgggcgttgg
     7381 ccggcgcctc gagcgccagc aggccttcgc ggcgtgcgag gtggctccag ctgacgatct
     7441 gctcgatcat gcgccgatag tcgatctgcg gcggccgcca gacccagcgg agcatgcgga
     7501 aaccgcggcc gaaggtgccg ctcggcgtct gcagcattac ggcaccgagc gtgccgccga
     7561 taacaatgac cagtgccggc agttgcgcca gcgacgaggt ctgcccaccg tcgagcaact
     7621 ggccgacgag cagcgcgccg atgccgacga tcagaccgaa gatactgagt ctatccacgt
     7681 cgccggctcc gcttaatggc gacgaccggt tccgacgccc cgactacggc gcgcaggcgg
     7741 gcgatagcct ggctgtgcag ctgacagacg cgcgattcgg tcacgccgag gacctcccct
     7801 atctcgcgca agttcagctc ctcgtcgtag tagagcgcca tcaccagctt ctcacgctcg
     7861 ggcaggtcgc cgatcgcacc gaccagtgat tcgcgcattc ccagttcttc caggattcta
     7921 gccggatcgg cctcatcgtc ggtgaaatga cgttcgagaa aatcgtcctc gtcgtcgctg
     7981 accatgtcct ccatgtgcag gacctggtgt ccacgcgcat cgagcagcag cttctggtat
     8041 tcggcgagcg acaggcctag gccgtcggcg agttcggtct cgctcggcgg tcggccaaaa
     8101 tcctgttcga gctgatgaat cagcgcatcg acccgccgca gttcgcggcg caggctgcgc
     8161 ggcagccagt ccgcctcgcg caggccatcg agcatggcgc cgcggatgcg ttgcgtggca
     8221 taggtttcga attgcgcgcc gaggccggtc tggtagcgcg acatcgcctc gatgaggccg
     8281 atcataccgt tctggatcag gtcgtcgata tgcacgctgg ccggcagacg ggccacgaga
     8341 tagtgcgcga tccgtttgac cagcggcgcg aagtgttcaa cgagctggtc cgattcgagc
     8401 tgaccggccg cggtatacat gggagtcttg ggccgctagc gaagcggctt cgcgcgcggt
     8461 gtcgttatgc gttggctcaa gtgtagcagt tgccgcatca ggtcctcgaa gccctccgga
     8521 tcgtcctcgc ttggccagta gagcagctcg gaagcaaact gacgcaatgc agtggccgcc
     8581 ggtgattcgg gaaaggcttc gacgaccgac tttgacagcc gggctgcacg gcgcatggcg
     8641 tcatcgctag gaataaccgc ggccagatcg agcatcgcca attcacgctg gcctgccacg
     8701 cgagcgaggt tggcgtagac ctgctcggca tcgccggcgc tgcgggcgcg gttgatcagc
     8761 acgcggaacc gacgacggcc gtactgctgg ctgacgccct tgatcaacac gtaggcagcg
     8821 gtgatcgaca ccggttgcgc ggtgagcgtc accacggttt ccgatgtcgc cagcgcaaag
     8881 ggcgagaacc ccagcgggtg ttggtggcgg gcgtcgacga gcacgacgtc gggtgcaact
     8941 tcgagatcgc cgacggctgc gaacagcgct tcctgctggg cggcggtgac ctgatcgagc
     9001 gcaccggcag cgcgggcggc cggcaatacg cgcaggcccg ggcgcaactg gatcagcgca
     9061 tcgcgcagac ggcaccgccc ggaaatgacg tcgagcaggt cggcgcgaac ggcgctgccg
     9121 aggctggcgg cgatacagtc ggcgccctcg ttctcgtcga cgatcagaac ctgccggccc
     9181 tgagcggcga gcgcggcggc gaggccggcg actgcctgcg tcttgccgac gccagcatcg
     9241 gcggcgagaa aggtcacgac acgcaggtgc tggccgccga acaggcgccg caggccggca
     9301 gcctgatcgc cgtgcggatc aggcatgtcc ggcaccggcg acgaggctgc ccatcagcag
     9361 cgccggctcg gcattgcgat gacggtgcgg cgagtcttcg ggaagcccgc gaaaggcgcg
     9421 gtgcgctaga taaagggcgt tgggctgatg caggtcctcg ggcacccgct ggccattcga
     9481 aacatagtgc agcggcaggc ggtggcgaat cacggcatcg agcgcgccgg caaggccgac
     9541 ggcctcgtcg accttgctga cgatgcagcc ggcgacgccc tgctgcccgt aggcgctgat
     9601 cacgtcgtcg agcgtatcgc tgcggctggt tgcggagagc aacaagaggc gcttgacctt
     9661 ggcgtcgccg agcatcgccg cctgggcggc gacgttcttg tcgcgctggc tcatgcccat
     9721 cgtgtcgatg atgaccatat gcttgccgga cagctcgcgc agcgtcgctt tcaattccat
     9781 tccgtcgcgc acgccatgaa ccggaacccc gaggatgcgg ccgtagatac gcagctgttc
     9841 gtgggcgccg atgcggtagc cgtcggtggt gaccagcgcg acacggccag cgccgtagcg
     9901 cagcacacag cgcgccgcca gcttggcagc cgtggtcgtc ttgccgacgc cggtcgggcc
     9961 gaccagcgcg tagacaccgc cttgatcaat caggtcgatc gacggtgcgg cgccgcgcag
    10021 ctcatgcgcc agggcatcct gcgcccagtc gatcgccgcg cgatcgccga ggccttcggg
    10081 aacgcggttg gcgctgccgc gcgcgaacat cggcgaaaag cccgcgtcga gcatgtgccg
    10141 cagcaaggca ccgcgagccg gatcctgttc ggtcatgtcg cgccaagcga agccggcgag
    10201 ctgctgttcg accatctgcc gaagcgccct cagttcttcc atgaccgcgt tgggcacgac
    10261 ctgtgccgat ggcgtctcgg tcgtggccgc cgccggcgca accccggggc ttcgcgagaa
    10321 gtcgacatgc ggccgcgtgg cggcggcagg cgcggtggct ttcggccgtc tgctcgccag
    10381 cgacacgcgg tatccctcgt cgtcgtcact accgtagcgc gatgtctcgc gcttcggctc
    10441 ccgcggcggc ggcgggttgg cccgtgcagg gacggtgacc gcggcgatct cggcggcgct
    10501 gaccgcagtg atctcgacgc caccggcgac gttgcggttg gtcaggatga ccgagtcttc
    10561 accgagcaat tccttgacct ggcgaagcgc ctcacgggcg ttgctggcga ctatcttttt
    10621 gacgttcatg cggttttacc cccgacgatc gaggtcactt tgatgattcg gttatccggc
    10681 acctcagcgt gcgagagcac cttgagctgc ggaacggcgc gacgcaggaa gcgcgcgaga
    10741 agaacccgga tgctggccgg caccagcaga actgccggca ggcccagtgc ttcctgacga
    10801 ccggcggcgg cgccggtttc gctgaccagc gtgtcggcaa gtcccggttc gatagcgccg
    10861 ccgcccgact gaccggccat tgcctgaacc agcacccgct caagcgttgg gtcgagcgac
    10921 atgacctgga gttctacgtt gcccgggtag agctgctggg tgatcgcgcg gccgagggca
    10981 attcgcactt gcgccgacag ctcttccggc gtttccatgc gtggcgcgtt ttcggcgatg
    11041 gtctcgatga tcgtgcgcat gtcgcggatc ggcacgcctt cgtcaagcag cgcctgcaga
    11101 accttttgca gcgtactgag ggcaacggtc ttcggcacca ggtcctcgac cagcttgggc
    11161 atttccttgg ccaggtggtc gagcagttgt tgggtctcct ggcgcccaag cagttcggaa
    11221 gcgtgcgaga gcaacaggtg attgaggtgc gtggcgacca cggtgctggc gtcgacgacg
    11281 gtgtaaccga aggcctgcgc ctgctcgcgg ttggccgggt cgatccacac cgcctgtagg
    11341 ccgaatgccg gatccttggc cgcggtgccc ggaaccgtgc cggcgacgcg gccagggttg
    11401 atcgccatca gctggcctgg gaaggcttcg ccggtgccga tctcgacgcc cttgagctgg
    11461 atgcggtagg ccgtcggccg caactcgagg ttgtcgcgga tgtggaccga cggcggcagg
    11521 aaaccgattt cctgcgcaaa gcgcttgcgg atgccgcgaa ttctccgcag cagttcgccg
    11581 tcctgcgcct tgtcgaccag cgggatcagt cggtagccga cctcgagtcc gagcacgtca
    11641 acgggtgcca cgtcggccca gctcgcctcg acttcggcaa cggtcggtgg cggtgctacc
    11701 acggcaggca cttcggcagc ggccaggctc tccttgcgca ggtagtgtgc agcgccaaac
    11761 aggatcgagg cgagcaggat gaagaccagg ttgggcatgc ccggaacgag gccgaggacg
    11821 ccgacgatgc cgccggcgac actgagcgcc cgcgggttgc tgaagagctg ggcgacgaac
    11881 tgctggctga cgtcggtatc gggttcgccg acacgcgtca ccaccatgcc cgccgccacc
    11941 gagatgatca gcgccgggat ctgcgcgacc aggccgtcgc cgatggtcag cagcgtgtag
    12001 gtcttggctg ccgtgccgac gtcgaggttg tgctgcagca tgccgacgac caggccgccg
    12061 acgacgttga tcagcatgat gatgatgccg gcgatcgcgt cgccacggac gaatttcgag
    12121 gcgccgtcca tcgcgccgta gaagtcggcc tcgcgcgaga tctcggcgcg ccggcggcgt
    12181 gcgtcttcct cgccgatcag gccggcgtta aggtcggcgt cgatcgccat ctgcttgccg
    12241 ggcatcgcat cgagcgtgaa gcgcgccgaa acttcggcaa cccgcccggc gcccttggtg
    12301 atgactacga agttgatgat cacgaggatc aggaagacga ctaggccgac cgtatagttg
    12361 ccgccaatca ggaagttgcc gaaggcctcg atcaccttgc cggcggcgtc ggggccggta
    12421 tggccgtcga gcagcacgat gcgggtcgag gcgacgttga gcgacaggcg cagcagcgtc
    12481 gtcaccagca acagcgtcgg gaataccgag aaatcgagcg gccgcaccgt cgacagcgcg
    12541 acgaggatga cgatgatcga cagcgcaatg ttgaaggtaa agaacaggtc aagcatgaag
    12601 gtcggcagcg gcaggaccat catcgccagg atcatgacga tgaggaccgg cgcggcagcc
    12661 tgccgcaggc tcagtttggt gaagtcgacg ccgagcactt gactagccat tcactgcacc
    12721 cggatcgagc tcggccggga cctcgatgtc cttcggcggc agcggatact cgccaccctt
    12781 ttcgcggtag cggttgagct ggaagatgta ggcgagtacc tcggcgacgg ctgcatacag
    12841 gcggatcgga atctgttcgc cgatttcgga ccagcggaaa agcgcccgcg ccagcggcgg
    12901 cgcctcgacc agcggcacgc cgtgcttggc gccaagctcg cggatcttgc cggcgatctc
    12961 gccccgaccc ttggcgatga tcttcggcgc ggccatgccc ggcttgtagg cgagcgccac
    13021 ggcgaagtgc gtcgggttgg tgacgatcac gtcggccttt ggcacctcgg ccatcatccg
    13081 ccgccgcgcc gcctcgcgct gcaggctgcg gatgcgcccc ttgacctgcg ggtcgccttc
    13141 catttccttg tgctcgcgct tgacctcgtc gcgggtcatc ttgagcttct cgtggtaatg
    13201 ccagagctgg aagggcacgt cgacggcaac gaccagcgcc atcgatccgg cgaccagcag
    13261 gaagctcagc agcacgaggt cgctcagctg ctgcagcccg tactcgggcg ccatcgtcgc
    13321 cagcagcacc agttcgtcga gatcgttcca gatcaccagc acggcgacgc cgccgaccac
    13381 gatcgccttg acgatggcct ttgcgagttc gaccagaccc tgcttggaaa cgatgcgacc
    13441 gaggccattg accggattga ggcggctgaa cttcggcgtc agcgccttcg gcgagaagat
    13501 ccaggcgccg aggaaggccg gcgagatcag cgccaccagc atcagaccgc cgaggagcgg
    13561 cagcagcgca atcatcgcgt cggcgaagga atcgatcagg cgggggaaca gcgattcgac
    13621 gccgttgagc agggtcgatt caatggtgag ggatttgcgc gcgccggcgg cgatccggct
    13681 ggccatccag ccgccgagga accagaggat tccgacggcc gaaatcatga tcagaaaagc
    13741 cgacagctcg cgcgagtggg ggacctgccc ctcctcgcgt gcttgctcta ggcgtctgcc
    13801 tgtcgctggt tctgtgcgtt cgagatcgct ttcttctgcc atagtcccag accggttgct
    13861 gcacgatacc ggccaggaat caaaaaatca agaaaaatta ggggtgtact aacggatgcc
    13921 gagaaaaaaa cttaagtgta cacattgagg taaacgtggt aagtcgttga aaaaatgggg
    13981 gcttgcgccc ccatctttta actcactgtt tttaaagact ttcaactgac ctcaagccgc
    14041 ttccgcgtac ccttggcctc gctttcgctc tgcccgaaat cagccagcaa ttccttgagg
    14101 tcgaggatca gcacgatctg gccgttggcc agcgtggtca cgccggcgac gccgcgcgga
    14161 cggaaatcct cgagcgactt gatcaccgcg tcgtcgcgcc cagcgaagct atcgacgccg
    14221 aggatgaagc tacggtcggc agcctgcatg agcacgccaa actccgggta gcgctcgctc
    14281 ggccagccga gcagacgcga cagctgaatc accggcagga tttcgccgcg aaccaccagt
    14341 gtcgcccgcc cgccaacctc ctgcacggtc tcgcgctcga tcggtaggat ctcgcgcacc
    14401 atcgacagcg gcagcgcaaa gggctgatcc tcaagcctga ccagcaggac cggcaggatc
    14461 gccagcgtca gcggcaagga aatcaggaag acgctcccgc gccccggctc cgagcgaatc
    14521 tcgatcgacc cattgagttt ctggatattg gtgcgaacca catccatgcc tacgccacgg
    14581 ccggagacac tggaaatctg cgccttgctg gaaaagccgg ggaggaagat cagattgaag
    14641 ctctgccgct cgtcgagcgt attggcctct tcctcgctga ccagtcgcct ttcgacggcg
    14701 atcgcgcgaa tccgctcggc gctcatcccc tgaccgtcgt cggcgacgat cagcatgatg
    14761 tggtcgccct cctgccgcgc ttcgaggcga acgacgctct ttgccggctt gccggcggcc
    14821 tcgcgctcct cgggcgactc gacgccgtgg tcgactgcgt tgcggatcag gtggatcaat
    14881 ggatcggcga gatcctcgat catcgtcttg tcgacttcgg tgtcctcgcc ggccagcacc
    14941 agttcgacat ccttgccgag ctggcgcgcc aaatcacggg cgatccgcgg gtatttctgg
    15001 aagaggcgcc cgatcggctg catgcgcgtc ttcatcacgg cgttctgcag gtcggagacg
    15061 agcaggtcga gctggctcac cgcctggtca agcgcgtgca gggtctcgct gtcgctgcgc
    15121 ccctgcagga tgtcggcgcg cagactggtc aggcggttct tggtcaggcc gatctcgccc
    15181 gaaaggttga gcacctgatc caggcgcgcc gtatcgacgc ggatggtcgc ctcgcggacg
    15241 acgacgccgc ggtcatcggc gcgccgcccc atcggcgccg aatcggttgc ccccggccgg
    15301 tcggtggcgc ggcgaccata gaccggcgcc gccggcttgc cctcggcgac gacggtcgtc
    15361 gtcgccgcag ccgccacggc ggtggaagcg gatgccgccg gtgccttgcc ggcagatgcc
    15421 gaggccccgg tcaccgccgc atgcatggcc tgccagtccg gcccgcccgc ggcagcggcc
    15481 ggctgagcgg gcgccggcgc ctgcccatca aggacgttgc gcaggccatt aagcatcgcc
    15541 accggtgccg gctgcggctg aacgcgctgg ccgagttcgc cgaacatgtc gcgcacgccg
    15601 cgggttgctt ccatgatcac gtccatgatc tcgggcgtca gcgcgagctc gccattgcgc
    15661 aggcggtcga agaggttctc ggtcagatga cagagcgtca ccagttcggt ggcgttgagg
    15721 aatcccgcac cgcccttgat tgtgtggaaa ccgcggaaga tgtcgttaag caaggccttg
    15781 tcgtggggcg cccgctcgag atcgacgagc ttgttgtcga cgtccgacaa cagctcgcgc
    15841 gcctcctgca ggaaatcctg caggagatcg tccatgccgc cgaaatcact catgccttgc
    15901 ctcccatcac gaacctcgcc gcactagccg ttccgctcaa aatccgagac tgtcgagcag
    15961 gtcatcgacc tgcgcctgat tgctcaccac atcggtccgc ccggccggat tgatgatcgg
    16021 accgttcagc aggctgctga cggcttcggt tcggcgctcg tcgggaatga tgtcgatcag
    16081 cagcgccatc atctgcttct cgagatcctg cgccagcgtc accaccttct tgattacctg
    16141 accggtaagg tcctggaaat cctgcgccat catgatttcc atgagctgcg cgttggtcgc
    16201 cgaaaccgac tctggaacct gccccttgag gaaggcgcga gtttcgtcgg ccagcgcccg
    16261 gaattcgtcg accgacagct cattggcgta gagccggtcc caccgtgcgg caagcccact
    16321 gccggccgcc ccgagcgaat cctgaacggg catcgccaca tcggtcgcat tgagcacgcg
    16381 acacgctgcc tgctcggtca gcgtcgcgac gtaggcaagc cggtcggtcg catcgggaat
    16441 cgcctcaacc gtcttcgaca agagcttgtc gtaacccagc ccgcgcagca tgtcgtgcag
    16501 ttgccgcgcc atctggccga cgcggttgaa ggcctgactg gaattggcgg cgcctgccgc
    16561 agccggtgca cccggggccg cactgggctg cccggcctcg ccggcgatcg aatcgaacaa
    16621 ggcctgcagg tcgtccgagt cgcccgaggg gctgatcgcg ggtgccggcg ccggtgccgg
    16681 cgtaccgctc gatgccgcga cgctgtcgaa aagagcctgg agctcgtcgg agtcgcccat
    16741 ggaggtcatt tcgggcatcg tcagaccccc atcttctcga aaatcttctg cagcttctcg
    16801 ctcagcgtcg ccccggtgaa gggcttgacg atatagccgc tggcgccggc ctgcgccgcc
    16861 atgatgatgt tttccttctt cgcctcggcg gtgatcatca gcaccggcag atgcttcagc
    16921 gcgtcgctgg cgcggatcgc ctggagcagc gccagcccgt ccatgttggg catattccag
    16981 tcggtgacga cgaaatccac gtcgccggcc tgcagcttct gcagcgcgac ctggccatcc
    17041 tcggcttcgt cgacgttggt gaacccgagt tccttgagca gattgcggac aatccgccgc
    17101 atcgtcgaaa agtcgtcgac gaccaggaac ttcatcttct gatcaggcat ctcgttggtg
    17161 ctccactctc agtttttatt ggtttcagcg ctcgagcagg gggcgcagcg tatcggcaag
    17221 gattcccgag tcgaacttcg ccacatagcc gtcgacgcct accgccttgc ccatcgccct
    17281 gttggcttcc gacgacagcg acgaatgcat gatcaccggc acaccgtcga aacgcgggtc
    17341 ggccttgatg ttcttggtca gcacgtagcc gtccatctcg ggcatctcgg cgtcgacgag
    17401 gattacccgg atgtcgtcgt gaaccgtgcg cccggtctgc tgcgcatgcg ccgcgatcgc
    17461 ctgcagccgc gtccacgcct ccataccgtt ggtcgcgtgc ttgtgcttga cgcgcagctt
    17521 gtcgagcacc tcgctgatct tcttgcgcgc gacgaccgag tcgtcgacga agaacatcgt
    17581 cacgtcttcg ctgacgttag ccggcggaat atccacaatg atcgcctcgc cgaaggcgtt
    17641 ggcaagaatc tgctcgacgt cgagaatcga caccagctgg ccgttctcga gttcgatgat
    17701 cgcggtgatc agccctgaat tgttgctcag caccgactcc ggcgccttca cgcgctccca
    17761 gtcgacgcgg atgatcctgt cgacatcatg aacgaggaac ccgacggtcc gcttgctgta
    17821 ctcggcgacc atcatcgacg aacccagctc gctcggcgga tcgtcgagat cgatgaagtg
    17881 caccagcgcc agcaccggaa tcacgttgcc gcgcagcgaa atcaggccct cgacgccctt
    17941 gggcatattc ggcgtgcgcg tgatccgcgg cgcccgtccg acttcgcgga ccttgaagac
    18001 gttgatgccg aacagctcgc gcgtgcccag cgagaaaagc aggatttcca tctggttgga
    18061 gccggctagg cgcgtgcgtg cgtcgacgct ctccagcagg ttcttgcgtt ccaccaaatc
    18121 agtggccatg tttcactccc tgagggttcc gcgcctcaga cggcgggaac gatgtcctga
    18181 ccgatgccga gccgacgcgc caccacctcg gaaagccgct gcggctcgaa cttcgacacg
    18241 tactcgtcga cgccgactga ctcgccgagc gtgcggttcg acccacccga cagcgacgaa
    18301 tgcatgacca caggaatccc ggcgaagcgc gggtcgctct ttactttctt ggtcagcatg
    18361 tagccgtcca tctctggcat ctcgacgtcg gtgaggatca ggctgatcag cgcactgact
    18421 ggtttgccct gcgccgcggc aagcgatgca acgcgttcga gttcatccca cgccgcccgg
    18481 ccgttgatcg acgacatgta attcagaccc agcgcctcga aagtccgctc gagctgcttg
    18541 cgcgccaccg ccgaatcgtc ggcgaagaac accgtcgcat cggacttggg tagcggcttg
    18601 agcgcgcggt agatcatttc gtcgtccatc tgtgccgttt cggcgagcac cttctcgacg
    18661 tccatcatca tcaccagtcg accgtcggcc agctcggtca ctgccgtgac caacccgccg
    18721 agttcggcgg tcagcatcgg tggcggcaca cgcatgctgc tccagtcgag ccgcaggatc
    18781 gtgtcgaccg actcgacgag gaagccctgg gtcatgccgt tgtattcggt gacgatcatg
    18841 atgtcgcgcg gcgtttcggt gcgcacgcac gcgtacttgg cgagatcgac caccggcacc
    18901 agcgcaccgc gcaggctgac cattccctcg acggcatccg gcatttcggg cgcggcagtc
    18961 accggcggcg tccgcatcac ttcgcggact ttgaagacgt tgatgccgaa ggtttcgcgg
    19021 cgtccgttgc gcgaatcggt gccgagcgtg aacagcagga tctcgagctt gttcgtcccc
    19081 gccagcttgg tccgcgcgtc gatgcttttc agtagttcag acatggaagt ccccgtgaga
    19141 cccgacccat tgctttggta ggaaccgaag gataccgtcc gaacgccccg ctttgtagcc
    19201 ctgcgggccg aatttgcaac ctaatgccgc tatcggcccg atccgatgac ggaccactgt
    19261 agccgcggat caggcgcccg ccgccaacgc cagcatgcgc ggggcaattc gatcgagcgg
    19321 cagagcctcc tcaaccgccc cgatcattgc cgcttcgcgc ggcatgccgt agaccacgca
    19381 gctggcctcg tcctggccca aggtccgccc ccccgcggat cgaatcgcca gcaagccggc
    19441 ggcgccatcc ttgcccatgc cggtaaggat gaccccgacg acattacggc cgacaactgc
    19501 tgccgccgag ttgaagagca cgtcgaccga gggcatgtgg cgattgaacg gaccgctcgg
    19561 gtcgagttcg gtgacgtagc agcccggctg cgaacggatc cgcagatgag ccgagcccgg
    19621 cgcgatgtag acagtgccgc cgcgcaccgg cacgccagcc tgcgcttcga tcacctgcgg
    19681 cgcacacagg ccgtcgaggc gcttggcgaa ggaaccggtg aaggtcggcg gcatatgctg
    19741 gacgaccagg atcggcggac agtcgcgcgg caccgcggtc agcacttcct tcagcgcctc
    19801 ggtcccgccc gtcgaggcgc cgatgaagat gagccggctc acccgcgcct cgcgctgccc
    19861 accggctgat agacggtctt gcccagcgac cggaacagat cggcggcgtg caggaagctc
    19921 tcggagtgcc ctgcgaagag caagccatcg ggagccagca ccttggcgaa gctcgacagg
    19981 acacggtact gcgtcgcctt gtcgaagtag atcatcacgt tgcggcagaa gatgacatcg
    20041 acggtgccgc ccagcgacca gctggcatcg agcaggttga tctgccggaa gtcgacgatt
    20101 cgccgcagct cggcgcgaac cgtgaagctc ttgccggcgg ccgccggcga ctcggtaaaa
    20161 aagcgtcgca gccgctcggc ctcgaggcgc tcgacgcggt cgccgccgta ctggccggcc
    20221 cgcgcgaccg cgaggacgcc ggtatcgatg tcggaggcaa tgatgctggt tccgccggcg
    20281 ctgccgccga gtgcctcgaa gaccgtcatc gcaatcgagt acggctcctc gccggtggac
    20341 gccgcgcagc accagatgcg cgtcggccgg ccaccgcgct tcttgaggaa gtcggcgagc
    20401 agcgggaaat ggtgcgattc acgaaagaac gaggtcaggt tggtcgtcag cgaattgacg
    20461 aactgctccc attcggcacc gcctgtgcgt tcgagtcgat cgaggtaggc cgcgaagctc
    20521 ggcgaattgg tcgcccgaag ccgccgcgcc agacggctgt agaccatgtc ctgcttgaga
    20581 tcggaaagcg agatgccggc cttctgatga atcagacggc ggacgcgatc gaaatcggcc
    20641 ggcagaaact cgaactcgcg caccatcgac gcggcgccgg atccgggcgt ccggactggc
    20701 ggcagggtca cgcgcgcctc gcggtcgacg gcaactactg cgcgcccggc gcggcaacgg
    20761 ccggcgccag ctgctcggcc gccacccggt cgccggtgcc caggcgcaag ccgccggagt
    20821 cctgcctgaa catcgcctgc tcggcctgct ggttgaggac gacgatgctg atccggcggt
    20881 tgatcgggct caacggatcg ttgcggtcga agagcaccgt cgaggccagg ccgaccacgc
    20941 gcagcacctt gaattcgtcc atgccgccgg cgactagctc gcgccgagct gcgttggccc
    21001 ggtccgacga cagctcccag tttgagtagc cgaggttgcc gccgaagaac tgcgccgagt
    21061 cagtatgccc ggaaaggctg acgcgattgg gcacgccatt gagcaggcgg ccgatctcgg
    21121 taagcagctg ccgcgtatag ggtttcagct cggcgctcgc ggtatcgaac atcggtcggt
    21181 tctgctcgtc gacgatctgg atctgcaggc cgtcggaggt gatgtcgagc agcaactggt
    21241 tcttgaactg ctgcagcgac ggcgtcttgg tgatcaggtc ctcgatgttc agcttgagtt
    21301 gctcgaggac ttccttctcg cgctcgcgcg actcgccgtc gtcgcccttg ccgccctgcg
    21361 gcgtgtagcg atcacgcgtc gagacatccc cgcggcgaag ctggccggca gtcttggtga
    21421 tgtcggtgcc gccgcccttg atgatgctcg acgcgtcgcc ggaacccgag ccgccgaaga
    21481 aggcgacctt catcggcgtc ttgaagtact cggcaatgcc ctcgaaatcg ccctttgccg
    21541 tcgagccgat cagccacatc agcaggaaga aggccatcat cgcggtcacg aagtcgg
//
LOCUS       DATDAA010000423         5360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_64995,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000423 DATDAA010000000
VERSION     DATDAA010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64995"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VIS73_12715"
     CDS             <1..729
                     /locus_tag="VIS73_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5764062.1"
                     /translation="FGAADPIGRTIRIGQRPFTVIGLLEAKGQSLSGRDQDDMVLVPL
                     TTGQRQIFGSRFEDRVRMFYVQGKSEQQMVEAEQAITALLRQRHRLRDGQPDDFYIRN
                     LAAIADSAAETARVMSLLLGGIASISLLVGGIGIMNIMLVSVTERTREIGLRRAIGAR
                     QKDIRVQFLLEAVLVSFAGCLVGLLAGIGIALAANAFLDTPILIAGRDLLIAVAVSGA
                     TGLMFGYFPAQRAAGLDPIEALRQ"
     gene            complement(790..1863)
                     /locus_tag="VIS73_12720"
     CDS             complement(790..1863)
                     /locus_tag="VIS73_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764063.1"
                     /translation="MNTSVFQKLPLAVALAAMPLATHASPAGTLLFAKGGTQIVDPSG
                     VSRIGKAGDLLQPGERVVTPDGAVCQIKLWDGTLVGVRPDTELKVELPEQASARAKSV
                     IQVLKGGLRVINLDLRDGRTRLPVTLQSPTGVVDLTNADAESRVVSPETLKKSGVKDP
                     GLYTRVQIGKGTIRTGTGEVPIAIRTVAFTPMIDVAPTLVTASGSLFTSTQLSSTTLS
                     TTTLTTATVDPKLTTSLSTTTLDPKLTTSLSTTTLDPKLTTSLSTTTLEPILSTSTLS
                     TTFTSPTLATSTYTAPTTTTTLSSPTLSTTYIAPTTYTAPTTTTTMVSSTTLSSPTLT
                     TTTTTSGTTTTFTSKTGSTLTLR"
     gene            complement(1891..3141)
                     /locus_tag="VIS73_12725"
     CDS             complement(1891..3141)
                     /locus_tag="VIS73_12725"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEY5764064.1"
                     /translation="MSSISSPLVRHNSLRLAVCGLSLLVAAAYSLSARAAEAAGTVMA
                     STGQVEVIDSGGGARKVARGSPLNQGDRIVTGRNGLVQIRMADGSYLSVRSNTEMALD
                     RFEVDMANDHKASSFLISLVKGGLRAITGLIGRSNPDAYKVRTPTATIGVRGTDHEPM
                     YIPDASPVFSALGAPGVYDKVNDGESYIQSAAGIVALKVGQIGHVGRPQEVPVLLKVV
                     PSFYQVDVKIDARPKEDFSVRRPLPTAAKPLLLRPTISSRVATPAAKLDPAATTSVIG
                     GTTTLAPKLSNTTTLSTSTLSTTTKDLTSSTTTLDPSLSTSTLSTTTLTSPTLSTSTT
                     TLDPSLSTSTLSTTTLTSPTLSTTTTTSPTTTISPTLSTSTLSTTTLTSPTLSTTTTT
                     TTTSPTLLNSTTLTSPTLSTTIQK"
     gene            complement(3154..4524)
                     /locus_tag="VIS73_12730"
     CDS             complement(3154..4524)
                     /locus_tag="VIS73_12730"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEY5764065.1"
                     /translation="MPVLGRYFVAGISRLLRTVASVVLLAGLSTAALAAIDTAILERA
                     ETLIQSGKADEAYLLLEPHELGNAGDLVFDYLFATAALESGRPSRATFIYERILAVEP
                     GYVGVRADMGRAYYALGDYARAKIEFETVLAVQNLPPDLRSTVEQYVSQAEARAKSKR
                     TIASVYTELAWGYDTNIAGATGARSIVITGLPPPNILDLNPPDTRTQDHYLAVALGGE
                     ITHQLSDVWAGFVGGDIRSRRYRNFDQIDNWSGDGRVGFNYAGGRWLLRGTLSGGQYT
                     QNSDRLRDSIGLGFDWRYAVSNSSQITLNLGQTRYTYVSAANRANDNDTLSGSIGWLQ
                     AIGDGSTLLSLSFAGGTETVRTARSDGDRSFGGPRFLVQTSIDESVGAFVTGGATGSS
                     YDTFNDSFLVTREETLYDAAAGVTFALKKGVALRPQLTYVRNKSNIVLFDSTRTDFSL
                     NLRLDW"
     gene            4754..>5360
                     /locus_tag="VIS73_12735"
     CDS             4754..>5360
                     /locus_tag="VIS73_12735"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY5764066.1"
                     /translation="MENVEHSTHLLRDGAVLLGAALVFVLAFQRLRLGAILGYLLAGI
                     VLGNHGLGLISDAASKLQIAEIGVMLLLFVVGLELSPSRLWRMRRDIFGLGSLQVILS
                     ALALWAFIGSVTLFTWQAALALALPLALSSTAQVLPMMRARGELDTPVGRRAFSILLF
                     QDVSIVPLIAIIALLSRAPADPSAPPGWQLALLTIVAVAGLI"
BASE COUNT          923 a         1687 c         1706 g         1044 t
ORIGIN      
        1 ttcggcgccg ccgacccgat cggccggacg atccgcatcg gccagcgtcc gttcacggtc
       61 atcggtctgc tcgaagccaa gggacaaagc ctctccggcc gcgatcagga cgacatggtt
      121 ctggtaccgc taaccaccgg ccagcggcag atcttcggca gccgcttcga agaccgcgtg
      181 cgcatgttct acgtgcaagg aaagtccgag caacagatgg tcgaagccga gcaagccatc
      241 accgcgctgc ttcgccagcg ccaccgcctg cgcgacggcc agccagacga cttctacatc
      301 cgcaacctcg ccgcaattgc cgacagcgct gccgagaccg cgcgcgtgat gtcgctgctg
      361 ctcggcggca tcgcctcgat ctcactgcta gtcggcggca ttggcatcat gaacataatg
      421 ctcgtctcgg tcaccgaacg gacgcgcgag atcggcctgc gccgcgccat aggcgcgcgc
      481 cagaaagaca tccgcgttca gttcctgctt gaagccgtgc tcgtttcgtt cgccggctgc
      541 ctcgtcggcc tgctcgctgg catcggcatc gcgctcgcgg caaacgcctt tctcgatacg
      601 ccgatcctca tcgccggccg cgacctgctc atcgccgtcg cggtatcggg cgcgaccggg
      661 ctgatgttcg gttacttccc ggcgcagcgc gccgccgggc tggacccgat cgaggcgctg
      721 cgccagtagc cgcgcccaaa aaaaacccgc cccacggggt cgatatcaat caggcgaatt
      781 cgactgcact tagcgcagcg tcagggtcga accggtcttg ctggtgaagg tggtcgtcgt
      841 tccagaggtc gtcgtcgtcg tggtcaaggt cggcgaactg agcgtcgtgc tggagaccat
      901 ggtcgtggtc gtcgtcggtg ccgtgtaggt cgtcggcgcg atataggtgg tcgacaaggt
      961 cggcgaactc aaggtcgtcg tcgtggttgg cgccgtgtag gtggaggtcg ccagcgtcgg
     1021 cgaggtgaac gtggtcgaca gcgtcgaggt ggacaagatc ggctcaagcg tcgtcgtcga
     1081 caggctggtg gtgagctttg gatcgagcgt cgtcgtcgat aggctggtgg tgagcttcgg
     1141 atcgagcgtg gtcgtcgata ggctggtggt gagctttgga tcgaccgtcg ccgtcgtcag
     1201 cgtagtcgtg ctcagcgtcg tcgaactcag ctgggtcgag gtaaacaggc ttccgctggc
     1261 ggtcaccagg gtgggcgcga cgtcgatcat cggcgtgaag gcgaccgtac ggatggctat
     1321 cggcacttcg cccgttcccg tacgaatcgt gcccttaccg atctgcacgc gcgtatacag
     1381 ccccggatcc ttgacgccgg acttcttgag cgtttccggc gaaacgacgc gcgactcggc
     1441 gtcggcattg gttaagtcga ccacgccggt cggcgactgc agggtgaccg gcaggcgcgt
     1501 ccggccgtcg cgcagatcca gattgatgac ccgcaggccg cccttaagca cttgaatcac
     1561 gctcttcgcc cgcgccgatg cctgctccgg caactcgacc ttcaactcgg tatcgggacg
     1621 gacaccgacc agcgtgccgt cccagagctt gatctgacaa acagccccat ccggcgtgac
     1681 gacgcgctcg ccgggctgaa gcaagtcgcc ggccttgccg atgcggctga ctcctgacgg
     1741 atcgacgatc tgcgtaccgc ctttggcaaa aaggagggtt ccggccggcg acgcatgcgt
     1801 tgccagaggc atcgccgcca gtgcaaccgc caagggcaac ttctggaata cggacgtgtt
     1861 catggtgatc tccttcatgc gcaacaaccc ctacttctga atcgtcgtcg acagggtcgg
     1921 tgaagtcagg gtcgtcgaat tcaacagcgt tggcgaagtc gtggtggtcg tggtcgtcgt
     1981 gctcagggtg ggcgaggtca gcgtcgtggt gctcaaggtc gatgtcgaca gcgttggcga
     2041 aatggtagtc gtcggcgacg tggtcgtcgt cgtggacaag gttggcgagg tcagcgtcgt
     2101 ggtgctcaag gtcgatgtcg acaatgacgg atccagtgtg gtggtgctag tactcagagt
     2161 aggcgacgtt aaggtcgtcg tgctcagcgt tgaggtcgac aagcttgggt caagcgttgt
     2221 cgtcgagctc gtcagatcct tggtcgtggt gctgagcgtg ctcgtactga gcgtcgtcgt
     2281 gttcgacagc ttgggggcca gcgtcgtcgt cccgccaatc acgctcgtcg tcgcagccgg
     2341 gtcgagcttg gcggccggcg tcgcgacgcg actgctgatc gtcggccgca aaaggagcgg
     2401 cttggctgcc gttggcagcg gccggcgcac actgaaatcc tctttcgggc gtgcatcgat
     2461 cttcacgtcg acctgataga agcttggcac gaccttgagc agcaccggca cctcctgcgg
     2521 cctgccgacg tggccgatct ggccgacctt cagcgcaacg atgccggcgg cgctctggat
     2581 gtaggactcg ccgtcgttga ccttgtcgta gacgccgggc gcacctaatg ccgagaatac
     2641 cggcgaggca tcagggatat acatcggctc gtggtcggta ccgcgcacgc cgatcgtggc
     2701 cgtcggggtc cggaccttgt acgcatcggg attgctgcgc ccaatcagac cagtgatcgc
     2761 acgcagccca cccttgacca gcgaaatcag gaagctcgac gccttgtggt cgtttgccat
     2821 atcgacctcg aagcgatcga gcgccatttc ggtgttcgag cggaccgaca ggtagctgcc
     2881 gtcagccatg cgaatctgca ccaggccatt tctcccggtg acgatccgat cgccctggtt
     2941 cagtgggctg ccgcgcgcga ccttgcgcgc gccgccaccg ctgtcgatca cttcaacctg
     3001 ccctgtcgat gccattacgg ttcccgccgc ttcggcggcc cgcgcactga gcgagtaggc
     3061 ggcggcgacc agcaggctca gcccgcacac ggcaagacgc agggaattat ggcgaacaag
     3121 cggacttgag attgacgaca cccggaactc tccctaccaa tcaagacgca gattcaacga
     3181 gaaatcggtt cgcgtcgagt cgaacaggac gatgttgctc ttgttcctaa cgtaggtgag
     3241 ttgcggccgc agtgcaacgc ccttcttgag ggcaaacgtg actcccgccg cggcatcgta
     3301 aagggtctct tcgcgggtca cgaggaagga gtcgttgaac gtgtcgtagc tcgaaccggt
     3361 cgcccctccg gtgacgaagg cgccgaccga ttcgtcaatg ctggtctgga ccaggaaacg
     3421 cggcccgccg aagctacggt cgccgtcgct gcgagcagtc cgaaccgttt cggttccgcc
     3481 cgcgaaactg aggctgagca gcgttgaccc atcgccgatc gcttgcagcc acccgatcga
     3541 accgctcagc gtgtcgttgt cgttggcacg gttggcggcc gatacgtagg tgtagcgggt
     3601 ctggccaaga ttaagtgtga tctggctcga attgctcacc gcatagcgcc aatcgaatcc
     3661 cagcccgatg ctatcgcgga gtcggtcgga attctgcgtg tactgcccgc cgctaagggt
     3721 cccgcgcagt agccagcggc cgccggcgta gttgaaaccg acgcgcccgt cgccggacca
     3781 gttgtcaatc tgatcgaagt tccggtaacg gcggctgcgg atgtcgccgc cgacgaaacc
     3841 agcccagaca tcgcttaact ggtgggtaat ttcaccgcct aaggcaacgg caagatagtg
     3901 atcctgggtc ctcgtatcgg gcggattcag gtcgaggatg ttcggcggcg gcaggcctgt
     3961 aattacgatg ctgcgggcac cggtcgcccc ggcgatgttg gtatcgtaac cccaggccaa
     4021 ctccgtataa acgctagcaa tcgtccgctt cgacttcgcc ctcgcctccg cctgactaac
     4081 gtactgctcg acagttgagc gcaggtctgg cggaaggttc tgcaccgcca acaccgtttc
     4141 aaactcgatc ttcgcccgcg cgtaatcgcc gagcgcgtaa taggcccgcc ccatatcggc
     4201 acgaacgccg acatagccgg gttcgaccgc cagaatccgt tcgtagatga aagtcgcgcg
     4261 gctcggcctg ccgctttcca gcgccgccgt cgcgaacaga tagtcgaaga cgagatcgcc
     4321 ggcattgccg agttcgtgcg gctcgagcag caggtaggcc tcgtcggcct tgcctgactg
     4381 gatcagtgtc tcggcacgtt cgagaatcgc cgtgtcgatt gcggccaacg ccgcagtcga
     4441 gagaccggcg agcaggacaa cgctggcaac tgtgcgcagg agccggctaa tgccggcgac
     4501 gaagtagcgt ccgagaactg gcaagatttg acccccgacg agtaggttca ggctgttgac
     4561 catgcgctgc cggcaggtcc gcgcccggca gggtttcgat tccagcggga cattctaagt
     4621 cgcgcgacga tgccaagtgc aactaagcac aatgcgaggc gcttgaaaag cgaatagttc
     4681 gcatccttgg taaaattcgc accctcttct gcttcggtca ttcgggcgcc catcctttcc
     4741 ggcgccacgc cccatggaaa acgtagaaca ctcaacgcac ctgctgcgcg acggtgcggt
     4801 gctgcttggc gccgccctcg tctttgtcct tgccttccag cggcttcgcc tcggagcaat
     4861 tcttggctac ctgttggctg gcattgtcct cggcaaccac gggctgggcc tgatctcaga
     4921 tgctgcgagc aagctgcaga tcgccgagat cggcgtcatg ctgctgctct tcgtcgtcgg
     4981 cctcgaactg agcccctcca ggctctggcg catgcgccgc gacatctttg gattgggcag
     5041 cctgcaggtc atcctgtcgg cgctggcgct gtgggcgttc atcggcagtg tcacgctgtt
     5101 tacctggcag gctgcactgg cgctggccct gccgctggcc ttatcgtcga cggcgcaggt
     5161 actgccgatg atgcgcgcgc gcggcgaact cgatactccg gtcggtcgac gcgccttctc
     5221 gatccttctg ttccaggacg tgtcgatcgt gccactgatc gcgatcatcg cactgctctc
     5281 gcgtgccccc gccgatccca gcgccccacc tgggtggcaa ctggcgctgc tgaccatcgt
     5341 tgcggttgcc ggcctgatcg
//
LOCUS       DATDAA010000424        16349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_100426,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000424 DATDAA010000000
VERSION     DATDAA010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 16349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16349
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100426"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VIS73_12740"
     CDS             <1..597
                     /locus_tag="VIS73_12740"
                     /inference="COORDINATES: protein motif:HMM:NF012246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HEY5764067.1"
                     /translation="WCRKNGLLLHVHRAMHGVIDRNPNHGINFRVLAKMLRLMGGDHL
                     HSGTVVGKLEGDREATLGWVDLMRDRHIKADRSRGIFFDQDWGPMPGVIPVASGGIHV
                     WHMPALVSIFGNDSVLQFGGGTIGHPWGNAAGAAANRVALEACVQARNEGRAIEKEGK
                     DILTNAAKHSPELKVAMETWKEIKFEFDTVDKLDVQHR"
     gene            637..993
                     /locus_tag="VIS73_12745"
     CDS             637..993
                     /locus_tag="VIS73_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HEY5764068.1"
                     /translation="MSNVQDYKSRLSDPASRMMGTFSYLPQMTPEQIRKQIEWIIKNG
                     WNPAIEHTEPAHAMSNYWYMWKLPMFGETDVDRILSELEACHKANPDNHVRLLGYDNF
                     KQSQGANMVVYRGKAV"
     gene            1070..1873
                     /locus_tag="VIS73_12750"
     CDS             1070..1873
                     /locus_tag="VIS73_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEY5764069.1"
                     /translation="MTDLIEQHRIQEEPYYRAVADEVALFEASYAAKMPMMLKGPTGC
                     GKTRFVEHMAWKLRKPLITVACNEDMTASDLVGRFLLDAAGTRWQDGPLAVAARHGAI
                     CYLDEVVEARQDTTVVIHPLTDNRRVLPLEKKGELLRAHADFHLVISYNPGYQSLMKD
                     LKQSTKQRFGALDFNYPEHATEVEIVAHEAAISAEVADKLVAIAERARNLKGHGLDEG
                     ISTRMLIHAGSLIARGVDPVAACRVALVRPITDDPDMRDALDAAVSTFF"
     gene            1963..4290
                     /locus_tag="VIS73_12755"
     CDS             1963..4290
                     /locus_tag="VIS73_12755"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HEY5764070.1"
                     /translation="MSVNLKDYAELIEELPENHRQLLEAEWVEAVRILSPRGLDNWLK
                     SAIALQRMGRGSDIVVAWIEEVPAIAREVGEDVIPDFAQQCLQFASRTSGAVIALVVG
                     TAPTAARRLGDPELFRAYLQLLNVLLGQAARGLRPMLEHLDQLLGVLTLGGLRRWAMW
                     GAQAHRTNFEEQTRYFGLESEEARAMLQQERKGTLFVDIHRRIGMYLRALWGRDFFMR
                     PTAGDFDSREGFKPFIADYFLHLPDAYDDWEGADGHKIAGLDLYRAAAAHCAAHVVVI
                     SERIDADHLNLLQQTCIGLFEDARAETLAIRRFPRLREFWRRFHAPRAEGSGIADYLQ
                     RIACALLDPAYDDADPLVTWAREEFAGSDLSTNQATCGMGLALADEFRRRALPFSPHH
                     DVQSVPYRDDNRYLWDYEEYSYEQAIAAGYTQEQQVRKYVSVSEMVNEVEVETAGDDA
                     QEIWVCATELFDDDGISFNQKEGREPVAEPRHYDEFDYHIQLNRPAWATVLEKRPKFG
                     DLAVVDRIIDDNRKLTQRLKYLLDALQPQGVQRIRKLEDGDEVDINAAIRALVDIRMG
                     QQPDPRVMMRSVRKTRDIAVQVLLDLSESTNDRVAGQDYTVLELTRAATVLLGEAIDK
                     VGDAFAVHGFYSDGRHNVFYQRYKDFDQPWNDLPKARLAGMEGRFSTRMGAAIRHAGH
                     HLAQVRAAKKLLIVITDGEPADVDVRDPQYLRYDAKKAVEEVGRAGVTPFCLTLDPRA
                     DQYVARIFGQRNYLILDHVERLPERLPQLYAGLTR"
     gene            4333..4695
                     /locus_tag="VIS73_12760"
     CDS             4333..4695
                     /locus_tag="VIS73_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764071.1"
                     /translation="MYARLADFPVLDRYPFRIPAAAWNLWYRLQHRHPPVAQRRIDLP
                     GLEPLQMILEERLWVCVDPTLADAPVIAWQRFESTGRSDLSAAMPCTVLQYHFGASRF
                     RDRIIAVMAEQIALLPKG"
     gene            4745..6127
                     /locus_tag="VIS73_12765"
     CDS             4745..6127
                     /locus_tag="VIS73_12765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY5764072.1"
                     /translation="MSEPKTRSRPAADTGELRSRLPLVLATLAIAAGLALAAWLLFSF
                     VRPAPPRVVTLVTGTPGGAYALFAERYRSALAREGIELRLLPSSGSVENLRRLKTEPD
                     VDLGFVQSGIASEPESTDLAVLGSMYLEPVWIFHRSPRPLTRLTELQGKRVAVGAPGS
                     GTQLLALQLLANTGISIGDPGLLALDEAEAAEALLAGRIDAAFLVAAPQAPAVEQLLG
                     TPGIQLLDLAHAEAYARRFPHLAPITLPAGAIDLGLPNPTSDRRLLGATASLIARADL
                     HPAIVNLLLQAAREVHGAPGLFQRAGEYPQQLSGRDLPVHPAAKRYFEYGPPFLQRYL
                     PFWLAVLADRLLIALLPIVALAIPLFRIAPAIYAWRKRSRIYRWYGELKFLEADIHEN
                     FSADRLDAYLEDLERIEELANRRRVPLAFSNELYTLREHIQLVRRELMQRAERAGATA
                     LTTARPGREG"
     gene            6521..8119
                     /locus_tag="VIS73_12770"
     CDS             6521..8119
                     /locus_tag="VIS73_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5764073.1"
                     /translation="MAASDTSNLMDGRAYRESLKRYKPTVFVDGQRIESVVDAPSLQP
                     GINAIAVSYDFAHDPALAPLMRARQTASGKTVNRMLHIDESAGDLLNKLEAVRVLCQE
                     TGCAQRYLAHDALNGILQAVSKIDDAKGSSEYAARFAAYLQRVQDKDLALGIAMTDAK
                     GDRSKRPNQQANPDAYVHIVERNVTGIVISGTKAIVTAAPYVHEFLVMPSRNMGEEDA
                     DFAVCCAVPIDAAGITIVARPAGRPGEKPEHGAPLFSRKYGQSTGVVIFDKVLVPWDR
                     VFLAGEWEFSGDVTYNYATHHRHSCIAARAGFGDLLIGAGALMCEANGLDPERKTNLR
                     DPMVELIKITEGFYACGVAASVYAVRDPHSQSFMPEPVYSNIGKLLLSTQIYDMHRLA
                     HEVSGGLIVALPGPDEDHNPATAATLSEVLRANPAVPYDKRIEVSRFIEDLTSSYQSG
                     WYSLISLHGGGSPAAMKQEIWRNYPVGNKVDLVERLLDRGVLADDQRSITRNKQPGRC
                     CDAGCSQPGQPFMVPLPGNGKRRR"
     gene            complement(8607..10058)
                     /gene="tldD"
                     /locus_tag="VIS73_12775"
     CDS             complement(8607..10058)
                     /gene="tldD"
                     /locus_tag="VIS73_12775"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEY5764074.1"
                     /translation="MTAIHLPPSLLQAHAALLTPFDLDESAISKALGHMLRHRVDYAD
                     LYFQQSRSEAWSLEEGIVKSGSFSIDQGVGVRAVSGERTAFAYSDDISEAALTDAAST
                     VRAIAAAGQSRRVPIIRRGEQPALYVGHDPLATLASADKVALLERLEGYARACDPRVR
                     EVMAHIAGEHEIILIAGSDGRLAADMRPLVRVSISVIVEENGRREQGSAGGGGRTDYA
                     MFTDELLKTYAQQAVHQAVVNLAARPAPAGPMTVVLGSGWPGILLHEAVGHGLEGDFN
                     RKGSSAFSGRIGQRVAAKGVTVVDDGTMPDRRGSLSVDDEGTPTGRTVLIEDGILKAY
                     LQDTMNARLMGVAPTGNGRRESYAHMPIPRMTNTVMLGGKRDPEEIVASVKRGIYASN
                     FGGGQVDITSGKFVFSMAEAYLIEDGKITHPVKGATLIGNGPDALTRVKMIGNDLRLD
                     SGVGTCGKEGQSVPVGVGQPTLRIDGLTVGGTG"
     gene            complement(10121..10945)
                     /locus_tag="VIS73_12780"
     CDS             complement(10121..10945)
                     /locus_tag="VIS73_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY5764075.1"
                     /translation="MSDIGRLRIAALQMTSTPRVADNLKIVDELAAEAAARGAQLVAL
                     PEYYPIMGSSEDAKVYAREPFAAGGNSGPIQSHLKALARRHGLWVIGGSIPLEASTPD
                     KILNSMLVYTPTGELAARYDKIHLVGFQKGVERYDEAASIEPGHEIVAFDAPFGRVGL
                     SICYDLRFPELYRAMGRPDLIVVPAAFTETTGRAHWEILLRARAIENQCYVLAVGQGG
                     QHESGRMTHGNSMIIDPWGEILDRLPKGQGVVIADFDRERIREVRASLPALDHRTM"
     gene            complement(10942..15015)
                     /locus_tag="VIS73_12785"
     CDS             complement(10942..15015)
                     /locus_tag="VIS73_12785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HEY5764076.1"
                     /translation="MAKRTAGVRMVKRRARPAPGWLARTGILLYWLVAVTAIVFSVLL
                     IALRLWGLPAIEAERERIVAAASEAIGVPMRIDNLRAGWQGINPRFEFFDVRIAGADG
                     QTRLTLPRVEAVVSWLSLAVLDLRLARLQIDGPNLVIERDAGGRLSVAGIALDDSQGG
                     ESPAADWLMRQHRLVITDGELTWLDRQRNAPPLHLNDIAFRLDNLGPWHRFALRGTPP
                     SAMASPLDLRGSLFGFDHDLEADWRGELYIDVDRVDLDAWQQWVNVPFELRSGGAGIR
                     AWVDIARSGPVGITADLRLAGTDLRLSKSLPLLSVELLAGRVGVRRHKAGFRVDLSRL
                     QLHTADGIHVDPVDADIAWQFEADGGSDGHVEVARIDLGTLARVAEFLPLDEGTRTLL
                     VDYQPAGRLSNLRASWLGKAAGLEAYSLISDFEDLGVRARGRFPGIQGLSGNLEANER
                     WGRLFLNSGPSGLELPAVFPEPLMAFDSLAGGVRWTRRDKLLEVELQALNFAGIDAVG
                     TARGRYEYRGDGPGYLDLSAELTRAEAPAVWRYLPHVVPPGTRDWLREALRKGIVDHA
                     ELRLEGELMHFPFEQGDGTFVVNARVRNATLAFQPGWPAVESLDADLRFAGSRMEIVS
                     RAGSILGVPLRRTVVALPNLAGFDPVLSIDGGAAGPIPSFLAFLDESPIGGAIGGFYR
                     DWRAEGEADLKLGLSIPLARPAETSVEGSVEFRDARLKLFDFLPAVNRVRGKLAFSEK
                     GAEARDLRGDFLGSPAKVTVASGEGNTEVRANGTLRADAAYRHYGWGWLPYLGGEAAW
                     EARVRVPPDGRVLVDVSSDLTGLASSLKAPLNKTADEALPLRVSYQRSSAGDRLETTL
                     GKQIAADLRGQLRGERWQIARGAVAIGEPLALPAKGFELRATMPNFDADFWQRVLRTE
                     SANGKPLAASSSQAAADAADAQAAERAAGPEAQGWPMEAWPQLSLRFVRLRYAERDFN
                     EVLLEGRPQDARLSGRVEAKEALGTWAWRPSPSGPGQLSARLQRLAVPAVSKPEATET
                     GVGAVVEAAVDTARAVLAAPGELVGKPAAVPPSAPPSAPAATGVLRPRLPDLDLQADD
                     ARVGAMGLGRLELMARNVGDDWLIDRFISTAADGEISASGGHYNHGDDETRLSISVTA
                     SNLGTMLDRLGYGGAVGSGSGRLGGAVRWSGGIPDYAPGRLGGDVVVHAEKGRFRSLE
                     NRGARLLGLLSLQNLPRRLLFDFGDVVSEGIAFDSIDGRFAIADGVMKTEGFRIDSPS
                     GRVLMAGDIDLGRETQDLRLAIRPAVGSTVALGAAIFANPIVGVATLLAQKLFRDPLD
                     KAFAVEYSVRGPWSEPQVERIGQVGGAAPGTAPVPTP"
     gene            15176..>16349
                     /gene="glnE"
                     /locus_tag="VIS73_12790"
     CDS             15176..>16349
                     /gene="glnE"
                     /locus_tag="VIS73_12790"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEY5764077.1"
                     /translation="MSEHAQPESDRWALATTHSRFLARLLTVRPELPEMLAPWSSRPL
                     DAAAMRGLLGPLDDADEAALGLALRRLRQAVMAQVIVRDLADGAPLAEIVGTMTTLAE
                     LSVNAALGLLHRQLAAQYGEPLDSRGRPQKLIVIGMGKLGGRELNVSSDIDLIFVYPE
                     DGETAGPQVIDAYDFFFRLGKRLIGIISDITEAGQVFRVDMRLRPDGDSGPLVLPLDA
                     LENYLIAQGREWERYAWIKSRVLNEGDNAQADWIAALQGTTHPFIFRKYLDYGAINAM
                     RDLHVQIRREVARKEMATHVKLGPGGIREIEFIAQVFQLIRGGRDATLRSRPTLKVLG
                     LLVERRLLPAETREELVAAYDFLRRVEHRLQYFEDAQTHQLPSTDADCEMLARSMGM"
BASE COUNT         2754 a         5779 c         5296 g         2520 t
ORIGIN      
        1 tggtgccgca agaacggcct gctgctgcac gtacaccgcg cgatgcacgg cgtcatcgac
       61 cgcaatccca accacggcat caacttccgc gtgctggcca agatgctgcg cctaatgggc
      121 ggcgaccacc tgcactcggg caccgtcgtc ggcaagctcg aaggcgaccg cgaagcgacg
      181 ctcggctggg tcgacctgat gcgcgatcga cacatcaaag ccgaccgctc gcgcggcatc
      241 ttcttcgatc aggattgggg cccgatgccg ggtgtgatcc cggttgcgtc cggcggcatc
      301 catgtctggc acatgccggc gctggtatcg atcttcggca acgactcggt gctgcaattc
      361 ggtggcggca cgattggcca cccctggggc aacgccgccg gcgccgctgc caaccgcgtc
      421 gcactcgaag cctgcgtgca ggcgcgcaac gaaggccggg cgatcgagaa ggaaggcaag
      481 gacatcctca ccaatgccgc caagcacagt cccgaactca aggtggcaat ggagacctgg
      541 aaggaaatca agttcgagtt cgataccgtc gacaagctcg acgtccagca ccgctgaaca
      601 gcattcgaac cgacaacccg ttcaaaggag tttgcaatga gcaacgtaca agactacaag
      661 tcccgcctgt cagacccggc cagccggatg atgggcacct tctcctacct gccgcagatg
      721 acgcccgagc agattcgcaa gcagatcgag tggatcatca agaacggctg gaacccggcg
      781 atcgagcaca ccgagccggc gcacgccatg tccaactatt ggtacatgtg gaagctgccg
      841 atgttcggcg agaccgacgt cgaccgcatc ctctccgagc tagaagcttg tcacaaggcc
      901 aatcccgaca accacgtgcg actcctcggc tacgacaact tcaagcagag ccagggcgcc
      961 aacatggtcg tctatcgcgg caaggccgtc tgattccgct gtgctgtcct gccgtcaccc
     1021 gctgggggaa ccagtgaacg acggcaggtc ttcccgaccg gagatccaca tgaccgatct
     1081 gatcgagcag caccgcatcc aggaagagcc ctactaccgc gccgtcgccg acgaagtcgc
     1141 gctgttcgag gccagttacg ccgcgaagat gccgatgatg ctcaaggggc cgaccggctg
     1201 cggcaagacg cgctttgtcg aacacatggc gtggaagctg aggaagcccc tgatcaccgt
     1261 cgcctgcaac gaggacatga ccgcctccga cctggtcggc cgcttcctgc tcgacgccgc
     1321 cggcacgcgc tggcaggacg ggccgctggc ggttgccgcg cgccacggcg caatctgcta
     1381 cctcgacgaa gtggtcgagg cgcgccagga caccacggtg gtcatccacc cgctcaccga
     1441 caaccgccgc gtcctgccgc tcgagaagaa gggcgaactg ctgcgcgcgc acgccgactt
     1501 ccatctggtg atttcctaca accccggcta ccagagcctg atgaaggacc ttaagcagtc
     1561 gaccaagcag cgcttcggcg cgctcgactt caactatccc gagcacgcca ccgaagtcga
     1621 gatcgttgcc cacgaggccg cgatcagcgc cgaggtcgcc gacaagctcg tcgccatcgc
     1681 cgagcgcgcc cgcaacctca agggccacgg cctcgatgaa ggtatctcga cgcgcatgct
     1741 gatccacgcc ggcagcctga tcgcccgcgg cgtcgacccg gtcgccgcct gccgcgtcgc
     1801 gctggtgcgg ccgatcaccg acgaccccga catgcgcgac gcgctcgacg cagccgtctc
     1861 gaccttcttc taggtggcgc tgacgcggaa ccccgccggg ccgcgtccgc gcacaatcgc
     1921 cgcccgctgc gaccgcagga tggcaggagg aatgaagtca ccatgagcgt gaacctcaaa
     1981 gactacgccg agctgatcga ggaactgccg gaaaaccacc ggcagctgct cgaggccgag
     2041 tgggtcgagg ccgttcgaat cctctcgcca cgcggcctcg acaactggct gaagagcgcc
     2101 atcgcgctgc agcgcatggg tcggggcagc gacatcgtcg tcgcctggat tgaggaagtg
     2161 ccggccatcg cccgcgaggt cggcgaggac gtcatccccg actttgccca gcagtgcctg
     2221 cagttcgcct cgcgcacttc gggcgcggtg attgcgctgg tcgtcggcac cgcgccgacc
     2281 gccgctcgcc gcctcggcga tcccgaactg ttccgagcct acctgcaact gctcaacgtg
     2341 ctgcttggcc aggcggcgcg gggcctgcgg ccgatgctcg agcacctcga ccagctgctc
     2401 ggcgtgttga cgctcggcgg cctgcggcgc tgggcgatgt ggggcgctca agcgcatcgc
     2461 accaacttcg aggagcagac gcggtatttc ggcctcgaat ccgaagaggc ccgggcgatg
     2521 ctccagcagg agcgcaaagg cacgctgttc gtcgacatcc atcgccgcat cggcatgtac
     2581 ctgcgcgcgc tgtggggacg cgacttcttt atgcggccga cggccggcga tttcgattcg
     2641 cgcgaaggct tcaagccctt catcgccgac tacttcctgc acctgcccga cgcctacgac
     2701 gactgggaag gtgccgacgg gcataaaatc gccggcctcg acctctaccg ggccgccgcc
     2761 gcccactgcg cggcgcacgt cgtggtcatc agcgagcgca tcgacgccga ccacctcaac
     2821 ctgctgcagc agacctgcat cggactcttc gaggacgccc gcgcggagac gctggcgatc
     2881 cggcgctttc cccggctgcg cgaattctgg cgccgctttc acgccccccg cgccgaaggc
     2941 agcggcattg ccgactatct gcaacggatt gcctgcgcgc tgctcgaccc tgcgtacgac
     3001 gatgccgacc cgctggttac ctgggcacgc gaggagtttg cggggtccga tctcagcacc
     3061 aaccaggcaa cctgcggcat gggcctggcg ctggccgacg agttccgccg ccgcgcgctg
     3121 cccttctcgc cacaccacga cgtgcagtcg gtgccctacc gcgacgacaa ccgctacctc
     3181 tgggactacg aggagtacag ctacgagcag gcaatcgccg ccggctacac gcaggaacag
     3241 caggtacgca agtacgtcag cgtctccgag atggtcaacg aggtcgaggt cgagaccgcc
     3301 ggcgacgacg cgcaggagat ctgggtctgt gccaccgagc tgttcgacga cgacggcatc
     3361 agcttcaacc agaaggaagg ccgggagccg gtcgccgagc cgcgccacta cgacgagttc
     3421 gactaccaca tccagttgaa ccgcccggcc tgggcaaccg tgctcgagaa gcggccgaaa
     3481 ttcggcgacc tcgccgtcgt cgaccgcatc atcgacgaca accgcaagct cacgcagcgg
     3541 ctcaagtatc tgctcgatgc gctgcaaccg cagggcgtgc agcgcatccg caagcttgaa
     3601 gacggcgacg aggtcgacat caacgccgcg atccgcgcgc tggtcgacat ccgaatgggc
     3661 cagcagcccg acccgcgcgt gatgatgcgc tcggtgcgca agacccgcga catcgccgtg
     3721 caggtgctgc tcgacctctc ggaatcaacc aacgaccggg tcgccggcca ggactacacg
     3781 gtgctcgaac tgacccgcgc cgccacggtg ctgctcggcg aggcaatcga caaggttggc
     3841 gacgccttcg ccgtccatgg cttctactcc gacggtcgcc acaacgtctt ctaccagcgc
     3901 tacaaggatt tcgaccaacc ctggaacgac ctgcccaagg cgcggctggc cggcatggaa
     3961 ggccgcttct caacccgcat gggcgcggcg atccgccacg ccggccacca cctcgcgcag
     4021 gtccgcgcag cgaagaagct cttgatcgtc atcaccgacg gcgagccggc cgacgtcgac
     4081 gtgcgcgacc cgcagtacct gcgctacgac gccaagaagg ccgtcgagga agtcggccgc
     4141 gccggcgtca cgcccttctg cctgacgctt gatccgcgtg ccgaccagta cgtcgcgcgc
     4201 atcttcggcc agcgcaacta cctgatccta gaccacgtcg agcgcctgcc cgagcgccta
     4261 ccccagctct acgccggcct cacgagatga accgcccgcc gcgggcactg cgcgccgcat
     4321 ccgcgccgtt gcatgtacgc gcggctcgct gacttcccgg ttctcgaccg ctatccgttc
     4381 cggattccgg cggcggcctg gaacctctgg taccgcctgc agcatcgcca cccgccggtg
     4441 gcgcagcgac ggatcgacct gccgggcctc gaaccgctgc agatgatcct cgaggagcgg
     4501 ctgtgggtct gcgtcgaccc gacgctcgcc gacgcgccgg tcatcgcctg gcagcgcttc
     4561 gagagcaccg ggcgcagcga cctgtcggcg gccatgccgt gcaccgtgct gcagtaccac
     4621 ttcggcgcca gccggtttcg tgatcgcatc attgccgtga tggccgaaca aatcgccttg
     4681 ctgccgaaag gctgaatagg gcagccgcat ggacagcagc cccgccgctc gttaggctgc
     4741 cgccatgtcc gaacccaaga cccgttcccg tcccgcggcc gataccggcg aactccgttc
     4801 gcgcctgccg ctggtgctcg ccacgctggc catcgccgcc ggcctcgcgc tcgccgcctg
     4861 gctgctcttc agcttcgtcc gccccgctcc gccgcgtgtc gtcacgctgg tcaccggaac
     4921 accgggcggc gcctacgcgc tgtttgccga gcgctaccga agcgcactgg cgcgcgaagg
     4981 catcgaactt cgcctgctgc cctcgtcggg ctcggtcgaa aaccttcgcc gcctgaaaac
     5041 cgagcccgac gtcgatctcg gcttcgtgca gagcggcatc gccagcgagc ccgagtcgac
     5101 cgacctcgcc gtgctcggca gcatgtacct cgaaccggtg tggatcttcc accgcagccc
     5161 tcggccgctg acgcggctta cagaactgca gggcaagcgg gtcgccgtcg gcgcgccggg
     5221 cagcggcacg cagctcttgg cgctgcagtt gctcgccaat accggcatct cgatcggcga
     5281 tcccgggctg ctcgccctcg acgaagccga ggctgccgaa gcgctgctcg ccggccgcat
     5341 cgacgccgcc ttcctcgttg ccgcgccgca ggccccggcg gtcgagcagc tgctcggcac
     5401 accgggcatt cagttgctcg acctcgctca cgccgaagct tatgcgcgcc ggttcccgca
     5461 tctggcgccg atcacgctgc cggccggcgc catcgacctc ggcctgccca atcccacgag
     5521 cgatcgccgc ctgctcggcg ccaccgcctc gctgatcgcc cgcgccgacc tgcacccagc
     5581 catcgtcaac ctgctgctgc aggctgcccg cgaggtccac ggcgcccccg gcctgttcca
     5641 gcgcgccggc gaatacccgc agcaactgtc gggccgggac ctgccggtcc atccggccgc
     5701 caaacgctac ttcgagtacg gcccaccttt tctgcagcgc tacctgccct tctggctcgc
     5761 cgtgctcgcc gaccggctgc tgatcgcgct gctgccgatc gtcgcgctcg ccatcccgct
     5821 cttccgcatc gccccggcga tctacgcctg gcgcaaacgc tcgcgcatct accgctggta
     5881 cggcgagctg aagttcctcg aagccgacat ccacgaaaac ttctccgccg accgcctcga
     5941 cgcctacctc gaagacctcg aacgcataga agaactcgcc aaccgccgcc gcgtcccgct
     6001 cgccttcagc aacgagctgt acacgctgcg ggaacacatc caactcgtcc gccgcgagct
     6061 gatgcagcgc gccgagcgcg ccggcgcgac ggcgctgacg acagcgcggc ctggtcggga
     6121 ggggtaagcg gcgtgcgccg gttcaggcgt cagtcgcttg cccgtcactc aggcggtatt
     6181 ggcattgcag ggaaatcgtg gattgcccac gctggattgc ccgccttgaa ttaggggccc
     6241 agagaaatcg agcctgaccc ctttcccttt tattcgcctt ttattccggc ccgccggccg
     6301 tcccggttgc gaaacgcgac gcgggccgca acccggagac gcacgccccg cgcgtgatcg
     6361 agcgctgccc cctcagcagg cctgccgttg cgccgcgccg ccggcgttcc gttcttccgc
     6421 cgaacccagc gcttcaactt gccatcgagg gaatttcaca ccgccaactc tggtcgaagc
     6481 caactctatc ggctagacgc cgaacacgcg aggaatgaac atggccgctt cggacaccag
     6541 caacttgatg gatggcaggg cgtatcgcga atccctgaag cggtacaaac ccaccgtctt
     6601 cgttgacggg caacggatcg aatcggtagt cgacgcgccg tcgctccaac ccggcatcaa
     6661 cgccatcgcg gtcagctatg acttcgcgca cgacccggcc ttggcgccac tgatgcgggc
     6721 caggcaaaca gcctccggca agacggtcaa tcggatgctg catatcgatg aatcggccgg
     6781 tgacctgctg aacaagctgg aagccgtgcg cgtgctatgc caggaaaccg gctgcgctca
     6841 gcgttacctg gcccacgacg ccctgaacgg catcctgcag gccgtctcga agatcgacga
     6901 tgcaaagggc tcgtcggagt acgccgcgag gttcgccgcc tacttgcagc gcgtccagga
     6961 caaggacttg gcgctgggca tcgcgatgac cgatgcgaaa ggcgaccgca gcaagcgccc
     7021 gaatcagcag gcgaacccgg acgcctacgt ccatatcgtc gaacgcaacg tcaccggcat
     7081 cgtgatatcc ggcaccaagg cgatcgtgac ggccgccccc tacgtccatg aattcctcgt
     7141 gatgccgtcg cgcaacatgg gcgaggagga cgccgacttt gcggtctgct gtgccgtgcc
     7201 gatcgacgcg gcggggatca cgattgtggc gcgacccgcc ggccggccgg gggagaagcc
     7261 ggagcacggt gcgccgctgt tctcccgcaa gtacggtcaa tcgaccggcg tggtgatctt
     7321 cgacaaggtc ctcgtgcctt gggacagagt cttcctcgcc ggcgaatggg agttctccgg
     7381 tgacgtgacc tacaactacg cgacccatca ccgccacagc tgcattgccg cccgcgccgg
     7441 cttcggcgac ctgctcatcg gcgccggagc gttgatgtgc gaagccaacg gcctcgaccc
     7501 cgagcggaag accaacctgc gcgacccgat ggtcgaactc atcaagatca ccgaaggctt
     7561 ctacgcctgc ggcgtcgccg ccagtgtcta cgccgttcgt gacccgcact cgcagagctt
     7621 tatgcccgaa cctgtgtatt cgaacatcgg caaattgttg ctctccaccc agatctacga
     7681 catgcaccgt ctcgcgcacg aggtatcggg cggactgatc gtcgctttgc cgggccccga
     7741 tgaagaccat aacccggcaa cggcagcgac cctgtcggaa gtcctgcggg ccaatccggc
     7801 cgtcccctac gacaagcgga tcgaagtatc acgcttcatc gaggacctga cctcctccta
     7861 ccagagcggc tggtattcgc tcatcagcct gcatggcggc ggctctccgg cagcaatgaa
     7921 acaggagatc tggcgcaact atcctgtggg caacaaggtg gacctggtcg agcgcctgct
     7981 cgaccgcggc gtcctagctg acgaccagcg ctccatcacc cggaacaagc agcccggacg
     8041 ttgctgtgat gctggttgca gccagcccgg ccagccattc atggttccgc tgccaggcaa
     8101 cgggaaacgg cgccgttgag cgaaacggca acggacccga gccgcgagct tcgccatcga
     8161 acagtgctgc acggccgggt tgccgggccc aatgtgagcc acccagaggg tccttccgca
     8221 gtgaaaatcg agcctggccc ttattgctcc ccgccttgtt tagtgttgac aaccggctcg
     8281 tgccacgcgc agacaagttt gcggccgggg ccgcggtcgc ggcggcaacg aggatcgcgt
     8341 cgagcgcagc ggacgcgacg accggggcgg acgcgccgag cgcccggaga aagccgagcg
     8401 cgctgagcgg gtcgaacgcg ccgagcgccc ggaacgcgtc gagcggatcg agcgcgccga
     8461 acgcgactga tagccgcttt ccggccgccg gctgagccgg tggtcgaaag acgagcgaac
     8521 cccggcgatt tcggtcgtcg gggtttttgc gtcaactgcg cgaatttcag cgatgctgcc
     8581 cgtgggcgag acagcggccg gatagcttag ccggtgccgc cgaccgtcag cccatcgatg
     8641 cgcagcgtcg gctggccgac gccgaccggc acgctctggc cttccttgcc gcaggtgccg
     8701 acacccgaat cgaggcgcag gtcgttgccg atcatcttca cccgcgtcag cgcgtcgggg
     8761 ccgttgccga tcagcgtcgc gcccttgacc gggtgtgtga tcttgccgtc ttcgatgagg
     8821 taggcctcgg ccatcgagaa gacgaactta ccgctggtga tgtcgacctg gccgccgccg
     8881 aagttggagg cgtagatgcc acgcttgacc gaggcgacga tctcttcggg atcgcgcttg
     8941 ccgccgagca tgacggtgtt ggtcatgcgc gggatcggca tgtgtgcata cgattcgcgc
     9001 cggccgttgc cggtgggcgc tacgcccatc agccgcgcat tcatggtgtc ctggaggtag
     9061 gccttgagga tgccgtcctc gatgagcacg gtacggccgg tcggcgtgcc ttcgtcgtcg
     9121 accgagagcg aaccgcggcg gtcgggcatg gtgccgtcgt cgacgacggt gacgcccttg
     9181 gcggcaacgc gctggccgat gcggccggag aaggccgaac tccccttgcg gttgaaatcg
     9241 ccctcaagcc cgtggccgac cgcctcgtgc agcaggatgc cgggccagcc cgagccgagc
     9301 acgacggtca tcggcccggc cggcgccggc cgcgcggcga gattgacgac ggcctggtgc
     9361 accgcctgct gggcgtaggt cttcagcagt tcgtcggtga acatcgcgta gtcggtgcgg
     9421 ccgccgccgc cggccgagcc ctgctcgcgg cgaccgttct cctcgacgat gaccgagatc
     9481 gacacgcgca ccagcggccg catgtcggcg gcgagccgcc cgtcggagcc ggcgatgagg
     9541 atgatctcgt gctcgccggc gatgtgcgcc atgacctccc gcacccgcgg gtcgcaggcg
     9601 cgcgcatagc cttcgagccg ctcgagcagc gccaccttgt cggccgaggc aagcgtcgcc
     9661 agcgggtcgt ggcccacata aagcgccggc tgctcgccgc ggcggatgat cggcacgcgc
     9721 cgggactgtc cggctgcggc gatggcgcgc acagtgctcg cggcatcggt gagcgctgcc
     9781 tcgctgatgt catccgaata ggcaaaggcg gtgcgttcgc cgctgacggc acgcacgccg
     9841 acaccctggt cgatgctgaa gctgcccgac ttgacgatac cttcctcaag cgaccaggcc
     9901 tccgagcgac tttgctggaa gtagaggtcg gcgtagtcga cccggtgccg cagcatgtgg
     9961 ccgagcgcct tcgatatcgc cgattcgtcg aggtcgaacg gggtcagcag cgcggcgtgc
    10021 gcctgcaaga gcgatggggg caagtgaatg gcggtcatcg ttacggttct ccgtggattc
    10081 ggtttgcggg ggcggcgggt ctttcccgct gccctccggc ctacatcgtg cggtgatcga
    10141 gcgccggcag gctggcgcgg acttcgcgga ttcgttcgcg atcgaaatcg gcgataacca
    10201 cgccctgtcc cttgggcagt cggtcgagga tctcgcccca agggtcgatg atcatgctgt
    10261 tgccgtgggt catgcgcccg ctctcgtgct ggccgccctg tccgaccgcg agcacgtagc
    10321 actggttctc gatggcacgc gcgcgcagca ggatttccca gtgggcgcgg ccggtggtct
    10381 cggtaaaggc agccgggacg acgatcaggt cgggccgccc catggctcgg tacagctctg
    10441 gaaatcgcag gtcgtagcag atcgacaggc cgacgcggcc gaaaggcgcg tcgaaggcga
    10501 cgatttcgtg ccccggctcg atgctcgcgg cttcgtcgta gcgctcgacg cccttctgaa
    10561 agccgaccag gtgaatcttg tcgtagcgtg ccgccagctc gccggtcggc gtatagacca
    10621 gcatgctgtt gaggatcttg tcgggcgtgc ttgcctcgag cgggatcgag ccgccgatca
    10681 cccacagccc gtggcggcga gccagcgcct tcaggtgcga ctggatcggc cccgaatttc
    10741 cgcctgccgc gaagggctcg cgggcgtaga ccttggcgtc ttcgctgctg cccatgatcg
    10801 ggtagtactc gggcagcgcg accagctgcg cgccgcgagc ggcagcctcg gcggcaagtt
    10861 cgtcgacgat cttcaggttg tcagcgacgc gcggcgtcga ggtcatctgc agcgcggcga
    10921 tgcgcaggcg gccgatatcg ctcatggcgt cggcaccggc gcggtgcccg gtgcggcgcc
    10981 gccgacctgg ccaatgcgct cgacctgcgg ctcgctccaa ggaccacgca ccgagtattc
    11041 cacggcgaag gccttgtcga gcggatcgcg gaaaagcttc tgggcgagca acgtggcgac
    11101 gccaacgatc gggttggcga aaatcgccgc gccgagcgcc accgtgctgc cgacggccgg
    11161 acggatcgcc agccgcaggt cctgcgtctc gcggccgagg tctatgtcgc cggccatcag
    11221 cactcggccc gaggggctgt cgattcggaa gccttcggtt ttcatcacgc cgtcagcgat
    11281 ggcgaagcgg ccgtcgatgc tgtcgaaggc gataccttca gagactacgt cgccgaaatc
    11341 gaaaagcaag cgccgcggca ggttctgcag actcagcaag ccgagcagcc gcgcaccacg
    11401 gttttccagc gagcggaaac ggcccttctc ggcatgcacg acgacatcgc cgccgagccg
    11461 acccggcgcg tagtctggaa tgccacccga ccagcgcacg gcgccgccca gacggccgct
    11521 gccgctgccg acggcgccgc cgtagccgag ccgatcgagc atggtaccaa ggttgctggc
    11581 agtgaccgaa atcgacagcc gcgtttcgtc gtcgccatgg ttgtagtgcc ctccgctggc
    11641 actgatctcg ccgtcggcgg cggtcgagat gaagcggtcg atcagccagt cgtcgccgac
    11701 gttacgcgcc atcagttcga gacggccgag acccatggcg ccgacgcgcg cgtcgtcggc
    11761 ctgcaggtcc aggtcgggca ggcgcggccg caggacgccg gtggcggccg gcgccgaagg
    11821 cggggcagac ggcggcaccg ccgctggctt gccgaccagc tcgccgggcg ccgcgagcac
    11881 cgcgcgcgca gtatcgacag cggcctcaac caccgcgccg acgccggtct cggtcgcttc
    11941 gggcttactg accgccggca ctgccagccg ctgcaggcga gcactcagct gaccgggccc
    12001 gcttggcgac ggccgccagg cccaggtacc gagcgcctcc ttcgcctcga cccggccgct
    12061 cagccgcgcg tcctgcgggc gcccctcgag caggacctcg ttgaagtcgc gttcggcgta
    12121 gcgcagccgc acaaagcgca agctgagctg cggccaggct tccatgggcc agccctgtgc
    12181 ctctggcccg gcggcccgtt cggccgcctg cgcatcggca gcgtcggcgg cggcctggct
    12241 agacgaggcg gcgagcggct tgccgttggc gctctcggtg cgcaacacgc gctgccagaa
    12301 atcggcgtcg aaattgggca tcgtggcgcg cagctcgaag cccttcgcgg gcagcgccag
    12361 cggctcgccg atggccactg cgccgcgcgc gatctgccag cgctcgccgc gcagctgtcc
    12421 gcgcaggtcg gcagcaatct gtttgcccag tgtcgtttcc agccggtcgc cggcgctgct
    12481 gcgttggtag ctcacgcgca gtggcagcgc ttcgtccgca gtcttgttga gcggcgcctt
    12541 cagactggag gccagcccgg tgagatccga gctgacatcg acgaggacac ggccatcggg
    12601 gggaacgcgc acacgcgcct cccaagccgc ctcgccaccg aggtagggca gccagcccca
    12661 gccgtaatgg cggtaggcgg cgtcggcgcg cagcgtgccg ttagcgcgga cctcggtatt
    12721 tccctcgccg ctggcgaccg tcaccttggc cggcgagccg aggaaatcac cgcgcagatc
    12781 gcgcgcctcg gcgcccttct cgctgaaggc gagcttgccg cgcacacgat taaccgccgg
    12841 caggaagtcg aacaacttga gccgcgcgtc gcgaaattca acactgcctt cgacgctagt
    12901 ctcggccggc cgcgccagag ggatcgacag gccgagcttg aggtcggcct cgccctcggc
    12961 gcgccagtcg cgataaaagc cgccgatagc gccgccgatt gggctctcgt cgaggaaggc
    13021 gaggaagctc ggaattggcc ctgccgcacc gccgtcgatg ctcagcaccg ggtcgaaacc
    13081 cgccagattg ggcagggcga cgacggttcg gcgcagtggc acgccaagaa tgctgccggc
    13141 gcgcgaaacg atctccatgc ggctgccggc gaagcgcaga tcggcgtcga gcgactcgac
    13201 ggccggccag ccgggctgga aggcgagcgt cgcgtttcgg actcgggcgt tgacgacgaa
    13261 ggtgccgtcg ccctgttcga agggaaaatg catgagttcg ccctcgagcc ggagctcggc
    13321 gtgatcgacg atgcccttgc gcaaggcctc gcgcagccag tcgcgcgtgc ctggcggcac
    13381 cacgtgcggc aggtagcgcc agaccgccgg cgcttcggca cgcgtcagct cggcgctgag
    13441 atcgaggtag cccggtccgt cgccacggta ctcgtaacgt ccgcgggcgg tgccgacggc
    13501 gtcgatgccg gcaaagttga gtgcctgcag ctcgacttcg agcagcttgt cgcggcgcgt
    13561 ccagcgcacg ccgccggcca gtgaatcgaa agccatcagc ggctcgggga agaccgccgg
    13621 caactccagt ccggacggtc ccgagttgag gaacagccgc ccccagcgtt cgttggcctc
    13681 gaggttcccg gagaggccct ggattccggg gaaacgacca cgagcgcgaa cgccgaggtc
    13741 ctcgaagtcg ctaatcaggc tgtaggcctc aaggcctgcg gccttgccca gccagctggc
    13801 acgcaagttg gagagccggc cggcgggctg gtagtcgacc agcagcgttc gcgttccctc
    13861 gtcgagcggc aggaactcgg cgacacgggc cagcgtacct aggtcgattc gcgccacctc
    13921 tacgtgccca tcgctgccgc cgtcggcctc gaactgccaa gcgatgtcgg cgtcgaccgg
    13981 gtcgacgtgg ataccgtcgg cggtgtgcag ctgcagccgc gacaggtcga cgcggaatcc
    14041 agccttgtgc cggcgcacgc cgacccgtcc ggcaagcagc tcgacgctca gcagcggcag
    14101 gcttttcgac aggcgcagat cggtaccggc cagccgcagg tcggcggtaa tgccgaccgg
    14161 gccgctccgg gcgatatcga cccaggcgcg aatcccggcg ccgcctgagc gcagttcgaa
    14221 gggcacattg acccactgct gccaggcatc gagatcgacg cggtcgacgt cgatgtagag
    14281 ttcgccgcgc cagtccgctt ccaaatcatg gtcgaagcca aacaggctgc cgcgcaggtc
    14341 gagcgggctg gccatcgccg acggcggcgt gccgcgcagc gcgaagcggt gccagggtcc
    14401 gagattgtcg aggcggaagg cgatgtcgtt gaggtgcagc ggcggcgcat tgcgctggcg
    14461 gtcgagccag gtcagttcgc cgtcggtgat gaccaggcga tgctgccgca tcagccagtc
    14521 ggctgccggg ctttcgccgc cctgcgaatc gtcgagcgca atgccggcaa ccgacagccg
    14581 cccgccggcg tcgcgctcga tcactaggtt cggcccgtcg atctgcaacc gcgccagtcg
    14641 caggtcgaga accgccagtg acagccagct gaccacagcc tcgacgcgcg gtagcgtcag
    14701 ccgcgtctgg ccgtcagcgc cggcgatgcg gacgtcgaaa aattcgaaac gagggttgat
    14761 gccctgccag ccggcgcgca ggttatcgat gcgcatgggc acgccgattg cctcgctcgc
    14821 ggcagcaacg atacgctcgc gctcggcttc gatcgccggc aggccccaca ggcgcagggc
    14881 gatgagcagc accgagaaga cgatcgcggt gaccgccacc agccagtaca gcaggatgcc
    14941 ggtccgcgcc agccaaccgg gtgcaggccg cgcgcggcgc ttcaccatgc ggaccccggc
    15001 ggtccgcttt gccattctcc gccgcctctc actacaatcc ctgccatcgc cgtttcgccc
    15061 gttacgcacc gcagcccgcc tgctggcgcg cgcgcgaagc gcgaattcta acccgccgac
    15121 gccactgtcc cgcccggcca gcccgcgaaa gaagcccgcc ttgtccgtcg aatcgatatc
    15181 cgagcacgcc caacccgaat ccgatcgctg ggcgctcgcc accacccaca gccgttttct
    15241 agcgcgactt ctcacggtcc gcccggaact gccggagatg ctcgcgccct ggtcttcccg
    15301 gccgctcgac gcggcggcga tgcgcggcct actcggacca ctcgacgatg ccgacgaagc
    15361 ggcgctcggc ctggcgctgc gccgcctccg gcaggcggtg atggcgcagg tgatcgtccg
    15421 cgacctcgcc gatggcgcac cgcttgccga aatcgtcggc accatgacga cgctcgccga
    15481 gttgtcggtc aatgccgcgc tgggcctgct gcaccgtcag ctcgccgcac agtacggcga
    15541 gccgctcgac agccggggac gcccgcagaa gctgatcgtc atcggcatgg gcaagctcgg
    15601 cggacgcgaa ctcaacgttt cgtcggacat cgacctgatc ttcgtgtatc ccgaggacgg
    15661 cgaaaccgcc gggccgcagg tgatcgacgc ctacgatttc ttcttccgcc tcggcaagcg
    15721 tctgatcgga atcatttccg acatcaccga ggccggccaa gtgtttcgcg tcgacatgcg
    15781 cctgcgcccg gatggcgatt cgggcccgct ggtgctgccg ctcgacgcac tggaaaacta
    15841 cctgatcgcg caggggcgcg agtgggaacg ctacgcctgg atcaagtcgc gcgtactcaa
    15901 cgagggcgac aacgcgcagg ccgactggat cgccgcgctg cagggcacga cgcacccgtt
    15961 catcttccgc aagtacctcg actacggcgc gatcaacgcg atgcgcgacc tgcacgtaca
    16021 gatccgccgc gaagtcgcgc gcaaggagat ggcgacgcac gtcaagctcg gccccggcgg
    16081 catccgcgag atcgagttca tcgcccaagt cttccagctt atccgcggcg gccgcgacgc
    16141 gacgctgcgc tcgcggccga cgcttaaggt gctcgggctg ctcgtcgaac gccggctgct
    16201 gccggccgaa acgcgcgagg aactggtcgc cgcctacgac ttcctgcgcc gcgtcgagca
    16261 ccgcctgcag tacttcgaag atgcgcagac gcaccagctg ccgagcaccg atgccgactg
    16321 cgaaatgctc gcccgatcga tgggcatgg
//
LOCUS       DATDAA010000425         2029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_450315,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000425 DATDAA010000000
VERSION     DATDAA010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450315"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..948)
                     /locus_tag="VIS73_12795"
     CDS             complement(37..948)
                     /locus_tag="VIS73_12795"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEY5764078.1"
                     /translation="MPLAMNREVFITCPVTGSGGTQDRSPHVPRSPKEIAESAIEAAR
                     AGAAVVHCHVRDPETGKASRKVEYYREVTERIRESETDVVINLTAGMGGDLVIGSPEQ
                     PLPLNEKGTDMASAEERMEHVAACLPEICTLDCGTMNFAEADYVMVNTPGMLRAMAKI
                     MKDLEVRVEIEAFETGHLWYAKQLVEEGLIEKPVMVQLCMGIPWGAPDDLNTFMAMVN
                     NVPPDWTFSAFSLGRNEMPFVAAAILAGGNVRVGLEDNLYLERGVLATNAQLVERAVT
                     IIENMGARVIGPDEVREKLKLQKRALP"
     gene            complement(963..>2029)
                     /locus_tag="VIS73_12800"
     CDS             complement(963..>2029)
                     /locus_tag="VIS73_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY5764079.1"
                     /translation="ADLSVSEFPFIWLRDNARENFHPDTKERTFDLTTVSADVAPDAV
                     ETDGASLQVRWPAQAGPSVYEGGWLYAHRPGRRRDGPARAGRLSWDAASMPSIPRADA
                     AACAGSPSELVNALLELKRCGLLIVDGLADDPEAGVDFGKQIGFLRETNFGVTYDVVN
                     RPEPNNLAYTSLGLPLHTDLPNQELIPGFQFLHCCRNDVDGGASVFADGLKICNDLKV
                     EAPADFEILSRTAIPWRFYDATADIRRHRPVINLDRNGGFEAFVFNAHIADLPDMPAD
                     TLYEFYPAYRRLMIRMRDPRYVIRYSLSPGEMVAFDNRRVLHGRTAFDAESGDRHLRG
                     YYIEQNEIDSRIRVLSRNYD"
BASE COUNT          332 a          640 c          677 g          380 t
ORIGIN      
        1 tgatcgcggc gactttcgcc ttcccggcgg attgcgtcac ggcagcgccc gtttctgaag
       61 tttcagcttc tcgcgcacct cgtccgggcc gatcacgcgc gcgcccatgt tctcgatgat
      121 ggtcacggca cgttcgacca gctgggcgtt ggtcgccagc acgccgcgct ccaggtacag
      181 gttgtcctcc agcccgaccc gcacgttgcc gccggcgagg atcgcggccg cgacgaacgg
      241 catctcgttg cggccgagcg agaacgccga gaaggtccag tcaggcggca cgttgttaac
      301 catcgccatg aaggtattga gatcgtcggg cgcgccccag ggaatgccca tgcagagctg
      361 caccatgacg ggcttctcga tcaggccttc ctcgacgagc tgtttggcat accagagatg
      421 accggtctcg aaggcctcga tctccacgcg cacctcgagg tccttcatga tcttcgccat
      481 cgcacggagc atgccgggcg tgttgaccat gacgtaatcg gcctcggcga agttcatggt
      541 gccgcagtcg agggtgcaga tctccggcag acaggcggca acgtgttcca tgcgctcctc
      601 ggcgctggcc atgtcggtgc ctttctcgtt gaggggcagc ggctgctccg gggagccgat
      661 gaccaggtcg ccgcccatgc cggccgtgag attgatgacg acgtcggtct cggactcgcg
      721 gatgcgttcc gtgacttcgc gataatactc gaccttgcgc gacgctttac cggtttcagg
      781 atcgcgaacg tggcagtgca ccaccgccgc gcccgccctg gcggcctcga tggcgctctc
      841 ggcgatttcc ttcgggctgc gcggcacgtg cggcgagcgg tcctgcgtgc cgccggaacc
      901 ggtgaccggg caggtgatga aaacttctcg gttcattgct aagggcatcg ggctgcctcc
      961 cgttagtcgt aatttctcga caggacgcga atacggctgt cgatttcgtt ttgttcaatg
     1021 tagtagccgc gcaagtgcct gtcaccggac tcggcgtcga acgccgtccg tccgtgcagc
     1081 acgcgccggt tgtcgaatgc caccatttct ccaggtgaaa gggaatagcg aatcacatat
     1141 cgtggatcgc gcatgcggat catcaggcgc ctgtatgcgg gataaaattc gtagagggta
     1201 tcggccggca tgtccggcag gtcggcgatg tgggcgttga aaacgaatgc ctcgaagccg
     1261 ccgttgcgat ccaggttgat gacaggccgg tgccggcgga tgtcagccgt cgcatcgtaa
     1321 aaacgccagg ggatggccgt gcggctgaga atctcgaaat cggccggcgc ctcgaccttg
     1381 aggtcgttgc agatcttcag tccgtcggcg aacacggagg cgccgccgtc gacgtcattc
     1441 ctgcagcagt gcaggaactg gaagccgggg atgagttcct ggttcggcag gtcggtatgc
     1501 agtggcagcc cgagcgacgt gtaggccagg ttgttcggct cgggcctgtt gacgacgtcg
     1561 taggtcacgc cgaagttggt ctcgcgcagg aagccgatct gtttgccgaa atcgacgccg
     1621 gcttccgggt catcggccag gccgtcgacg atcagaaggc cgcagcgttt cagttcgagc
     1681 agggcgttca ccagttcgga cggggaaccg gcgcaggccg ccgcatcggc tcgcggaatg
     1741 gacggcatcg acgccgcgtc ccaggagagg cgtcccgccc gcgccgggcc gtcccggcgc
     1801 cgaccgggcc ggtgggcata caaccagccg ccctcgtaga ccgacgggcc ggcctgcgcc
     1861 ggccagcgaa cctgcaggct ggcgccgtcc gtttccaccg cgtccggcgc gacgtccgcg
     1921 ctgaccgtgg tcaggtcgaa ggtgcgctcc ttcgtatccg ggtggaagtt ttcccgggcg
     1981 ttgtcccgca gccagatgaa cggaaactcg ctgacggaca ggtccgccc
//
LOCUS       DATDAA010000426         5929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_205946,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000426 DATDAA010000000
VERSION     DATDAA010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205946"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2932
                     /locus_tag="VIS73_12805"
     CDS             <1..2932
                     /locus_tag="VIS73_12805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEY5764080.1"
                     /translation="FRLACGMKLVALAQNRAGYGNLCALITLARRRAPKGQYQLTRGD
                     LDGLGAAGALPDCLVLWIPPEPLREASLDDGRWLAARLAGRAWLAVELLCGADDRSRL
                     DDLVDLSTACSLPLVAAGDVHLHVRKRRALQDTMTALRLNTTVDAAGFALYPNGERHL
                     RSRVRLARFYPPKLLAETLAIAERCTFSLDELRYEYPDDVAPAGSTPDAHLAAEVDAG
                     LARRYPAGVSTAVRAQVNYELKLIGELAYAPYFLTVHDIVGFARGRGILCQGRGSAAN
                     SAVCFALGITEVDPARASMLFERFISKERNEPPDIDVDFEHERREEVIQYLYAKYGRE
                     RAALTATVIHYRRKSALRDVGRALGFDATQIDALSASLAWWDKPEDWPERLAGVGLNP
                     ESPRVARWIALAKLLRGFPRHLSQHVGGFVISRGPLSRLVPIENAAMAERSVIQWDKD
                     DLDALGLLKIDILALGMLSAIRRALAMIAAQRGTPFALADIPAEDKATYDMLCRGDSM
                     GVFQVESRAQMAMLPRLKPRRFYDLVIEVAIVRPGPIQGDMVHPYLRRRDGREPIEAM
                     PPAVEAVLGRTLGVPIFQEQVMQLAIAAAGFTPGEADRMRRAMAAWRRRGGLEAFQDQ
                     LMAGLTANGYDESFAQRVCAQIQGFGEYGFPESHAASFALLVYASSWIKRHHPAAFLV
                     ALLNSQPMGFYRPSQLVQDAKRHGVVVLPVDVTASDVEATLVNASLQPARANATMTPA
                     VRLGLSTVRGLSSAAAERIVAARQAAPFADLVDFLGRCALARDDRDALTAADALRSLA
                     GHRRQAAWDAAAHHVRPALQGDLLADASAERETIALPAPREGEELIADYAHTGLTLRR
                     HPLSLLRPRLAAHRFLAVAEANNSPDSKLARVAGLVTCRQRPGTASGVIFVTLEDETG
                     WANVIVHAQLAEKQRAELLGARLLGVYGVIQREGRVVHLIAKRLVDCSAWLGELTVES
                     RDFC"
     gene            complement(2957..3409)
                     /locus_tag="VIS73_12810"
     CDS             complement(2957..3409)
                     /locus_tag="VIS73_12810"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEY5764081.1"
                     /translation="MNIQKQIAAFALALAASAAGAADGLVMVKSPYATKETMNRLAAQ
                     VEARGLKVFARIDHAAGAATIGATLRPTELLIFGNPQGGTPFMACAQTVGIDLPLKAL
                     VWEDVGGQVWLGYNAPAFLAKRHGVADCPVTAKLDGALAGIVGATVAK"
     gene            complement(3525..3914)
                     /locus_tag="VIS73_12815"
     CDS             complement(3525..3914)
                     /locus_tag="VIS73_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEY5764082.1"
                     /translation="MENQYGFGKTVQGDFAGTRERVIDELGKEGFGVLTEIDVAATMK
                     KKLDHDMAPYVILGACNPPFAKRAIEAEPTIGLLMPCNVVVRQDAAGIQVEFMDPGLM
                     SQMTANDAVVELAKEVREKIRRVMDAV"
     gene            complement(3939..4838)
                     /locus_tag="VIS73_12820"
     CDS             complement(3939..4838)
                     /locus_tag="VIS73_12820"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY5764083.1"
                     /translation="MDKCDSNDLPGEIPFPTSPVMPAQLAGSKRIHFRCHKGVSCFNA
                     CCKSIDISLTPYDIIRLKKRLGISSTEFLREYTFPYEFEKDGLAGVKFKPVESGTACR
                     FMVDEGCNVYEDRPTACRYYPVALLSMRRQDEYTDTQSYALVKEEHCRGHEDARELTI
                     DEYRAEQGLPEYDDLARGWRQLILKKKSSGPTVGKPSKRSLQLFFMACYDIDRFRAFV
                     ESESFSQVFVLPADERDAILADDVSLMQFAFRFLRQVLFGENTIELNGESVEQRRERL
                     RERQAQLEREAAERMAADEGYGD"
     gene            complement(5017..>5929)
                     /locus_tag="VIS73_12825"
     CDS             complement(5017..>5929)
                     /locus_tag="VIS73_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764084.1"
                     /translation="AWEDFLDMCVGQCGIWVGEDVDPREKNSELMPTEPYLLGSHSGC
                     CGIWVSGPTDVGAPTKEEYEGIPSHLPSGWNWGYRSMTTVKGLFTAGDGVGASGHKFS
                     SGSHTEGRLAAKAMVKYALDNADAKVELDADVNQLAEEIYKPVRTFLEHKDYTTAIDV
                     NPHYITPKMLQFRLQKIMDEYVAGVATYYNTNDKMLAVAEYKLGLLKEDAQKMRAKDL
                     HELLRAWENYHRILTAEAHMKHIQFREESRYPGFYYRTDKNFVDEKNWHCFVNSVYDK
                     NAKSWTVFKRKHVDLVDKSKLFKPAAH"
BASE COUNT          926 a         1972 c         1957 g         1074 t
ORIGIN      
        1 gttccgcctg gcctgcggga tgaagctggt ggcgctggcc cagaaccgcg ccggctacgg
       61 caacctctgc gcgctgatca cgctagcgcg gcgccgtgcg ccgaaggggc agtaccaact
      121 cacgcgcggc gatctcgatg gtctcggcgc ggccggcgcg ttgcccgatt gcctggtgtt
      181 gtggattccg cccgagccgc tcagagaagc cagcctcgac gacggccgct ggctcgccgc
      241 gcgccttgca ggccgcgcgt ggcttgcggt ggagctgctc tgcggcgccg acgaccgcag
      301 ccgcctcgac gacctagtcg atctgtccac agcctgcagc ctgccgctgg tggcggccgg
      361 cgatgtgcac ctgcacgtgc gcaagcgccg cgcgctgcag gacacgatga cggcgctgcg
      421 gctcaacacc acggtcgatg ccgcgggctt tgcgctctac cccaacggcg agcgtcacct
      481 gcgctcgcgc gtgcgcctcg cccgcttcta tccaccgaag ctgctggccg agacgcttgc
      541 catcgccgaa cgctgcacat tttcgctcga cgagctgcgc tacgaatacc ccgacgacgt
      601 ggcgccggcc ggcagcacgc ccgatgcaca cctcgccgcc gaggtcgatg ccggcctcgc
      661 ccgccgttat ccggcggggg tgagcacggc cgtgcgcgcg caggtcaatt acgagttgaa
      721 gctgatcggc gaactcgcct acgcgcccta cttcctcacc gtgcacgata tcgtcggctt
      781 cgcgcgcggc cgcggcatcc tctgccaggg gcgcggctcg gcggccaatt cggcggtgtg
      841 cttcgcgctc ggcatcaccg aggtggaccc ggcacgcgcc agcatgctct tcgagcgctt
      901 catctcgaaa gagcgcaacg aaccgccgga catcgacgtc gatttcgagc acgagcggcg
      961 cgaggaggta atccagtacc tctatgccaa atacggccgc gagcgcgccg cgctcaccgc
     1021 caccgtcatc cactaccggc gcaagagtgc gctgcgcgac gtgggccgcg cgctcggttt
     1081 cgatgcgacg cagatcgacg cgctctcggc ctcgcttgcc tggtgggaca agcccgagga
     1141 ctggcccgag cggctggccg gcgtggggct gaaccccgaa tcaccgcgtg tggcacgctg
     1201 gatcgcgctg gcgaaactgc tgcgcggatt tccgcgccat ctttcgcagc acgtcggcgg
     1261 cttcgtcatc tcgcgcgggc cgctgtcgcg cctggtaccg atcgagaacg cggcgatggc
     1321 cgagcgcagc gtgatccagt gggacaagga cgacctcgac gcgctgggcc tcttgaagat
     1381 cgacatcctc gcgctgggta tgctctcggc gatccgccgg gcgctggcga tgatcgcggc
     1441 gcagcgcggc acacccttcg cactcgccga cattcctgcc gaagacaagg cgacctacga
     1501 catgctctgc cgcggcgaca gcatgggcgt gtttcaggtc gagtcgcgag cgcagatggc
     1561 gatgttgccg cgcctgaagc cgcggcgctt ctacgatctg gtgatcgaag tggcgatcgt
     1621 tcgccccggc ccgatccagg gtgacatggt tcacccctac ctgcgccggc gcgatggccg
     1681 tgaaccgatc gaggcgatgc ccccggcagt ggaagcggtg ctcggccgca cgctcggcgt
     1741 accgatcttc caggagcagg tgatgcagct ggcgattgcc gccgccggct ttacaccggg
     1801 agaagccgac cgcatgcgcc gggcgatggc ggcgtggcgg cgacgcggcg ggctggaggc
     1861 gtttcaggat cagctgatgg ccgggctcac cgccaacggc tacgacgagt ccttcgcgca
     1921 gcgcgtctgc gcgcagatcc agggcttcgg cgagtacggc tttcccgagt cgcacgcggc
     1981 cagcttcgcg ctcctggtct acgcctcgtc gtggatcaag cgccaccacc cggcggcctt
     2041 tctcgtcgcg ctcctgaaca gccagccgat gggcttttac cgcccctcgc aactggtgca
     2101 ggacgccaag cgccacggcg tagtcgtact gccggtggac gtgacggcga gcgatgtcga
     2161 ggcgacgctg gtcaatgcca gcctccagcc ggcgcgggcg aatgccacga tgacgccggc
     2221 cgtgcggctc ggcctgtcga ccgtgcgcgg gctgtcgagt gccgccgccg aacgcatcgt
     2281 cgccgcgcgg caggccgcac ccttcgccga cctcgttgat tttctggggc gctgcgcgct
     2341 cgcccgcgac gaccgcgacg cgctcaccgc cgccgacgcg ctgcgctcgc tcgccggcca
     2401 ccggcggcag gcagcctggg acgctgcagc ccaccacgtc cgtccggcgc tgcagggcga
     2461 cctgctcgcc gacgccagcg ccgagcgcga aacgatcgcg ctgccggcgc cgcgcgaagg
     2521 cgaggaactc atcgccgact acgcgcacac cgggctgaca ctgcgccggc acccgctgag
     2581 cctgctgcgc ccgcgcctcg cagcccatcg cttcctcgcc gtggccgagg ccaacaactc
     2641 acccgatagc aagctcgcgc gcgtggcagg gctggtcacc tgccgccagc gccccggcac
     2701 cgcctcaggc gtcatcttcg tcacgctcga agacgaaacc ggctgggcca acgtgatcgt
     2761 ccacgcgcag ctggcggaaa agcagcgcgc cgaattgctc ggcgcacgcc tgcttggggt
     2821 ttatggcgtc atccagcgcg aaggccgcgt cgtgcacctg atcgccaagc gactcgtcga
     2881 ctgctcggcg tggctgggcg agttgacggt ggagagtcgg gatttctgtt gaggctggtt
     2941 cgccgtccgc cctcggctac ttcgcaaccg tcgcgccgac gatgccggcc agcgcgccgt
     3001 cgagtttggc ggtgaccggg cagtcggcga caccgtggcg cttcgccagg aatgccgggg
     3061 cgttgtagcc gagccagacc tgtccgccca cgtcttccca gaccagcgcc ttgagcggca
     3121 ggtcgattcc cacggtttgc gcacaggcca tgaagggcgt gccgccctgc gggttgccga
     3181 agatcagcaa ttctgtggga cgcagggtcg cgcctatcgt ggcggccccg gcagcgtgat
     3241 cgatccgcgc gaagaccttg agcccgcgcg cttcgacctg cgcggccaac cggttcatcg
     3301 tttccttggt ggcgtacgga ctcttgacca tcaccaggcc gtcggcagcg ccggcagccg
     3361 aggcggcaag ggccagggcg aatgcagcaa tctgtttctg gatgttcatg gctcgctccg
     3421 gttggtgagg aataaggccg accgcgtgcg ctcgccagcg tgacctgtcg atggtaacgc
     3481 cggaaccacg actgctttgc cgcgccaccg gcgcggagac ggacctatac ggcgtccatc
     3541 acccggcgaa tcttctcgcg cacttccttc gccagctcga cgacggcgtc gttggcggtc
     3601 atctggctca tcaggccggg gtccatgaac tcgacctgga tgccggcggc gtcctgacgg
     3661 acgacgacgt tgcagggcat gagaaggccg atggttggct cggcctcgat ggcgcgcttg
     3721 gcgaagggcg gattgcaggc accgaggatg acgtagggcg ccatgtcgtg atcgagcttc
     3781 ttcttcatcg tcgccgcgac gtcgatctcg gtgaggacgc cgaagccttc cttgccgagt
     3841 tcgtcgatca cgcgctcgcg ggtgccggcg aagtcgccct gcactgtctt gccgaatccg
     3901 tactggtttt ccatgttcgt tctcccgtgt tcgtggactc agtcgccgta gccttcgtcg
     3961 gcggccatgc gctccgctgc ttcgcgctcg agttgggcct gccgctcgcg caaacgctcg
     4021 cggcgctgtt cgaccgattc gccgttgagt tcgatggtgt tctcgccgaa cagcacctgg
     4081 cgcaggaagc ggaaggcgaa ctgcatcagc gatacgtcgt cggcgaggat cgcgtctcgt
     4141 tcgtcggcag gcaatacgaa aacctgcgag aaggactcgc tctcgacgaa ggcgcgaaag
     4201 cggtcgatat cgtaacaggc catgaagaaa agctgcaggc tgcgtttgga tggcttgccg
     4261 acggtcggcc ctgatgattt cttcttcagg atcaactgcc gccagccgcg cgccagatca
     4321 tcgtattcgg gcaggccctg ctcggcacgg tactcgtcga tggtcagttc gcgcgcgtcc
     4381 tcatgccccc tgcagtgctc ttccttgacc agcgcgtagg actgggtgtc ggtgtattcg
     4441 tcctggcggc gcatggacaa aagcgccacc gggtagtaac ggcaggcggt ggggcggtct
     4501 tcgtagacat tgcagccttc atcgaccatg aaacggcagg cggtgccgct ctcgaccggc
     4561 ttgaacttga cgccggccag cccgtccttt tcgaactcgt aggggaaggt gtactcgcgc
     4621 aggaactcgg tggagctgat gccgaggcgc ttcttcagcc ggatgatgtc gtagggcgtg
     4681 aggctgatat cgatcgactt gcagcaggca ttgaagcacg acacgccctt gtggcagcgg
     4741 aaatggatgc gcttgctgcc cgcaagctgc gccggcatga ccgggctggt gggaaacggg
     4801 atctcgcccg gaagatcatt cgaatcgcac ttgtccatcg tggtctctcg tcagttcctg
     4861 gagtcgcgcc gctcgctgct gcccgcattt cgcttgaggg ccgattcttg cacgcgatcc
     4921 gttgaaaaaa aagaaccggc accttctaaa aaaaaggacc gggtgcctca cggcactcgg
     4981 cccaagcaca acgcaaggag agattcgtgc gctgctctag tgcgcggccg gtttgaagag
     5041 tttgctcttg tccaccagat ccacgtgctt ccgcttgaac accgtccagc tcttggcgtt
     5101 cttgtcgtag accgagttta cgaagcagtg ccagtttttc tcgtcgacga agttcttgtc
     5161 ggtccggtag tagaagcccg gataacgcga ctcttcgcgg aactggatgt gcttcatgtg
     5221 cgcttcggct gtcaggattc ggtggtagtt ctcccaggcg cgcagcagct cgtgcaggtc
     5281 cttcgcacgc atcttctggg cgtcttcctt gagcaggccg agcttgtact cggcaacggc
     5341 aagcatcttg tcgttggtgt tgtagtaggt cgcgacgccg gccacgtact cgtccatgat
     5401 cttctgcagg cggaactgca gcatcttggg cgtgatgtag tgtgggttga cgtcgatcgc
     5461 tgtggtgtag tccttgtgct caaggaaggt gcgcaccggc ttgtagattt cctcggcgag
     5521 ctggttcacg tcggcatcaa gttcgacctt ggcgtcggca ttgtcgagcg cgtacttgac
     5581 cattgcctta gcagccagac ggccttcggt atgcgaaccc gaggagaact tgtgacccga
     5641 ggcgccgaca ccgtcaccgg ccgtgaacag gcccttgacc gtggtcatcg agcggtagcc
     5701 ccagttccag cccgagggca ggtgcgaggg aatgccttcg tactcttcct tggtcggcgc
     5761 accgacgtcg gtcgggcccg agacccagat gccgcagcag ccggagtgcg agccgagcag
     5821 gtagggctca gtcggcatca gttcggagtt cttctcacgc ggatcgacgt cctcgccgac
     5881 ccagatgccg cactgtccga cgcacatgtc gaggaagtct tcccatgct
//
LOCUS       DATDAA010000427         2816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_416212,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000427 DATDAA010000000
VERSION     DATDAA010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416212"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VIS73_12830"
     CDS             complement(<1..664)
                     /locus_tag="VIS73_12830"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY5764085.1"
                     /translation="MTTEALAAVARTREGALGIEQVMLDDPCRGELLVRLEACGICHT
                     DMKFRERLPLPAVLGHEGVGIVEAVGSDVRHARVGDRVLISYPFCTECPACRRAEPWC
                     CERIGELKFGGRRLDGSQPVSVQGAGVTSAFFQQSSFATHAITLEQSVIPVAEQLPAA
                     TLAALPCGVQTGAGAVMNTFGAGPGDSLMVFGAGAVGISAIRAARVAGLGTVICAEPL
                     AGR"
     gene            complement(707..1873)
                     /locus_tag="VIS73_12835"
     CDS             complement(707..1873)
                     /locus_tag="VIS73_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5764086.1"
                     /translation="MTFRFDPYSPEVDANPFPFYKTLRDEYPCFWSEEADMWVLSRYA
                     DISAALQDWKTYSSAKGNLMDELPNRAGATLGTTDPPRHDRLRMLIQMAVTKRALEHI
                     VEPTREAVNRYLDELEGQESFDFVTAYSARMTVDLLFFLFDMPEEDKREVRANAVLMV
                     QTDPVSRQKGPEHIAAYEFMTAYADKLIAIRKRKPGEDLLSQFILAEIDGEKLLDREV
                     RMTVATLLMAGIESLSGFMSMFAYNLANFPDARRALAADPSLIPDAVEESLRYNTSAQ
                     RFKRCLTRDVELHGQTMKEGDFVCLAYGSGNRDERQFENPDVYDIYRKPRGHLGFGRG
                     VHACLGAAIARLAVRVSFEEFLKRMPEFRQVERELPWTPSTNFRSPMRLVLARG"
     gene            1993..2583
                     /locus_tag="VIS73_12840"
     CDS             1993..2583
                     /locus_tag="VIS73_12840"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5764087.1"
                     /translation="MKSADLTGHCLSAIGGIALNIPRIETERLVLRAIDPARDFEPWS
                     KAMADAETVRFIGGQVLDRALAWRNMAMVVGHWSIRGYGFFSVEHRETGEWLGRVGPW
                     YPEGWPAPEIGWTIAREHWGRGYATEAARASLDYAFGTLGWRRAIHVILKGNERSAAV
                     AGRLGSVLVETRQGLPGVTDQEIEIYAQEAPGGQSD"
     gene            2576..>2816
                     /locus_tag="VIS73_12845"
     CDS             2576..>2816
                     /locus_tag="VIS73_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764088.1"
                     /translation="MTDATYAFESLLPIFAYFFIGLGLRRSGIANLEHAGFLFRILFL
                     VTLPALVFMSVSRANLGPTTVYLPLSGFLVNLSCAA"
BASE COUNT          499 a          937 c          888 g          492 t
ORIGIN      
        1 ggcggccggc gagcggctcg gcgcagatga ccgtgccgag gccggcaacc cgggcagccc
       61 tgatcgcgct gatgccgacc gcgcccgccc cgaagaccat caggctgtcc ccagggcccg
      121 cgccaaaggt attcatcacc gcgccggcgc cggtctgaac gccgcagggc aaagcggcga
      181 gtgtcgcggc cggtagctgc tccgcgaccg ggataaccga ctgctcgagg gtgatggcgt
      241 gcgtcgcgaa cgaggactgc tggaaaaacg cgctggtgac gccggccccc tgcaccgaga
      301 ccggctgcga tccgtcgagc cggcggccgc caaacttgag ttccccaatc cgttcgcagc
      361 accagggctc agcccggcga caggccggac attcggtaca aaacggatac gaaatgagga
      421 cccggtcacc aacccttgcg tgccggacat ccgagccgac ggcctcgacg ataccgacgc
      481 cctcgtggcc gagcaccgcc ggcaggggca ggcgctcgcg aaatttcatg tcggtgtggc
      541 agatgccgca ggcctcgaga cgcacgagca gttcgccccg gcacgggtcg tcgagcatga
      601 cctgctcgat gccgagcgcc ccttcgcgcg tgcgggcgac cgcggcgagg gcttcagtcg
      661 tcatgcgatc ggctacccat tacgtcggca ggaccggcct cgcccgtcag cctcttgcca
      721 ggaccaggcg catggggctc ctgaaattgg tggacggcgt ccacggcagc tcccgttcga
      781 cctgccgaaa ctccggcatg cgtttcagga attcctcaaa agacacccgg accgcgaggc
      841 gcgcaatcgc cgccccgagg caggcgtgaa ccccgcggcc aaaacccagg tgcccgcgcg
      901 gcttgcggta aatatcgtaa acgtccgggt tctcgaactg gcgctcgtca cggttgcccg
      961 atccgtaagc gaggcagacg aagtcgccct ccttcatggt ttggccatgc aattccacat
     1021 cgcgagtaag gcagcgtttg aagcgctgcg ccgaggtgtt gtagcgcagg ctctcctcga
     1081 ccgcgtccgg aatcagcgaa ggatcggccg ccagcgctcg gcgcgcgtcc gggaaattcg
     1141 ccaggttgta ggcgaacatc gacatgaacc cggacagcga ctcgatgccc gccatcaaca
     1201 gcgtcgccac ggtcatgcgc acctcgcggt cgagcagttt ttcgccgtcg atctccgcaa
     1261 gaatgaactg gctcagcagg tcttcgccgg gtttccgctt gcgaatcgca atgagcttgt
     1321 ccgcgtacgc ggtcatgaat tcgtaagccg cgatgtgctc cggtcccttc tgccggctta
     1381 ccgggtccgt ctgcaccatc aagacggcat tggcgcgcac ttcgcgcttg tcctcttccg
     1441 gcatgtcgaa aagaaagaac agcagatcca cggtcatgcg cgcggaatac gccgtgacga
     1501 agtcgaagga ttcctgccct tccagctcgt ccaggtagcg attgaccgct tcccgcgtgg
     1561 gctcgacgat gtgctcgagc gcccgcttgg tcacggccat ctggatcagc atgcgcaggc
     1621 gatcgtgcct gggaggatcg gtcgttccga gcgtcgcgcc cgcccggttc ggcaactcgt
     1681 ccatcaggtt gcccttcgcc gaggaatagg tcttccagtc ctgcagcgcc gcgctgatat
     1741 cggcgtagcg cgacaggacc cacatgtcgg cttcctcgct ccagaaacac ggatactcgt
     1801 cgcgcaaggt cttgtagaaa gggaacgggt ttgcgtccac ttccggggaa taggggtcga
     1861 aacgaaatgt cacgacagcc tccagcgcat tgtttccaag tctcccccgg gtcagtagac
     1921 gccatgccgg cgccggtagc ctcgattata atgcccgctt gcgtacacgc gattcagaat
     1981 gcttgcccgg ctatgaaatc ggccgacctg accgggcatt gcctgagcgc cattggaggt
     2041 atcgccctga atattcctcg aatcgaaacc gaacgcctgg tgcttcgcgc catcgatccc
     2101 gcgcgcgact tcgagccctg gagcaaggcg atggcggacg cggagaccgt gcgcttcatc
     2161 ggcggccagg tcctggaccg ggcgctcgcc tggcgcaaca tggcgatggt cgtcggccac
     2221 tggtcgattc ggggatatgg cttcttttcc gtcgagcacc gcgagaccgg cgaatggctg
     2281 ggccgggtcg gcccctggta cccggaaggc tggcccgccc cggaaatcgg ctggacaata
     2341 gctcgagagc actgggggcg cggctacgcg accgaggccg cacgggcatc gctcgattac
     2401 gccttcggaa cgctcggctg gcgaagagcg atccacgtca tcctgaaagg caatgagcgc
     2461 agcgccgccg ttgccggcag gctgggttcc gtcctggtcg aaacccgcca gggactgccc
     2521 ggcgtaaccg accaggaaat cgaaatttac gcgcaggagg cgccgggcgg gcaaagtgac
     2581 tgacgctact tatgccttcg aatcgctttt gccgattttc gcctatttct ttatcggcct
     2641 cgggttgcgc agaagcggca tcgcgaacct cgaacacgcc ggcttcctgt ttcgcatcct
     2701 gttcctggtc acgctcccgg cgctggtctt catgtcggtg tcgcgcgcaa acctgggccc
     2761 gaccacggtc tatctgccgc tcagcggatt cctggtcaac ctgtcctgcg ccgccg
//
LOCUS       DATDAA010000428         2861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_275221,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000428 DATDAA010000000
VERSION     DATDAA010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275221"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1341
                     /locus_tag="VIS73_12850"
     CDS             130..1341
                     /locus_tag="VIS73_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEY5764089.1"
                     /translation="MRVGLEVLLDDRQRRGALAGRRLALLAHPASLTRDLVHAIDAVA
                     ACTDLDLTAAFGPQHGLRGDKQDNMVESPDFVDPVHRVPVFSLYGEVRRPTAAMFEHF
                     DVLLVDLQDLGCRIYTFITTLRYVLELASVQGKAVWVLDRPNPAGRPVEGLTLRPGWE
                     SFVGAGPLPMRHGLTMGELARWFVATLKLDVELEVVAMGGWRPDSAPGYGWPTERSWV
                     NPSPNAPNLSMARAYGGTVMLEGTTLSEGRGTTRPLELFGAPDIDASAVIETMQALAS
                     EWLAGCRLRACWFEPTFHKHAGRLCSAVQIHVDDPAVYAHAGFRPWRLIALAFKAIRR
                     LAPGYPLWRDFPYEYEHDRLAIDLINGSPLLREWVDDSAAPPGELDRLARADEKAWVT
                     AREPFLMPEYR"
     gene            complement(1392..2117)
                     /locus_tag="VIS73_12855"
     CDS             complement(1392..2117)
                     /locus_tag="VIS73_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764090.1"
                     /translation="MNRNVFITLLAAAAVAACSPKSEEPAKPAAQSAAPAAPTTDLFA
                     KSASASPNAGTILSMEQAGIYTYAEVDTGAQKLWVAGGHIDAKVGDKVQWGDYAIMNN
                     FNSKALNRTFEQVLFVNNWGKEGAAMAPMAQHGTQPAGGAMAGHPPMAGHPPMGGGGQ
                     MPITGGANSGTVKTATTAAGYSYLEVDQSGKTTWIAVPEMRVKAGDKIAWAPGATMRN
                     FNAKSLNRVFDEIVFAGGAQVVQ"
     gene            complement(2214..2771)
                     /locus_tag="VIS73_12860"
     CDS             complement(2214..2771)
                     /locus_tag="VIS73_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764091.1"
                     /translation="MAATAHQPFLPFLVEELSRLHHNKNSGTLFATTINGRLAQFGLA
                     NGEIVFLSFQNVEGIEAVAAIRDQKMEVGTARFAPGRHRGAVLSLPPTAELLKLLAAG
                     AATTATERASTPSGSGAVLTPEARAIIEEELTEIGGPIAGMLCEELWEAGITNLGVAL
                     AELSKQLPDPNQAEALRHNVLRRLK"
BASE COUNT          409 a          915 c         1000 g          537 t
ORIGIN      
        1 tggcctgtcg gccgttcttg agcgtactga cggttcgctt gcctatttcg cactggcgca
       61 ccccagcgct cagcccgact tccacgatcg ccgtggctgg cgctgccgcc tggcggcaag
      121 ggctccggca tgagggtagg cctcgaagtc ctgctcgacg accggcagcg gcgcggcgca
      181 ctggccggcc gtcgcttggc gctgctcgcg catccggcat cattgacccg cgatctcgtc
      241 cacgcgatcg atgccgtcgc cgcctgcacg gatttggatt tgacagcggc attcgggccg
      301 cagcacggcc tgcggggcga caagcaggac aacatggtcg agtcgccgga tttcgttgat
      361 ccggtgcatc gggtgccagt gttcagcctt tacggcgaag tccgccggcc gacggcggcg
      421 atgttcgagc actttgacgt gctgctggtc gacctgcagg atctcggttg ccgtatctac
      481 accttcatca ccacgctgcg ctacgtgctt gagttggcct ctgtacaggg caaggcggtg
      541 tgggttctcg accgcccgaa cccggccggc cgaccggtcg agggactgac cctgcgtccc
      601 gggtgggaaa gcttcgttgg agctggaccg ctgccgatgc ggcacgggct gacgatgggc
      661 gagctggccc gctggtttgt cgcgacgctg aagctcgacg tcgaactcga ggtcgtggcg
      721 atgggcggct ggcggccgga cagcgccccg ggatacggct ggccgaccga gcgcagctgg
      781 gtgaatccga gtcccaatgc gcccaacctg tcgatggcac gcgcctacgg cggcacagtt
      841 atgctcgagg gaacgacgct ctccgaaggc cgcggtacga cgcggccgct cgagttgttc
      901 ggcgccccgg acatcgacgc gtcggcggtg atcgagacga tgcaggcgct cgcctcggaa
      961 tggctcgccg gctgccgcct gcgcgcctgc tggttcgagc cgaccttcca caagcatgcc
     1021 ggcaggctct gttccgcggt gcagatccac gtcgatgatc cggcggtcta cgcgcacgcg
     1081 ggcttccgac cctggcgact gatcgcgctg gccttcaagg ccattcgccg cctcgctccc
     1141 ggctacccat tgtggcggga cttcccctac gagtacgagc atgaccggct ggcgatcgac
     1201 ttgatcaacg gctcgccgct gctgcgtgag tgggtcgacg attcggcggc gccgccgggc
     1261 gagcttgacc gattggcgcg tgccgatgag aaggcttggg taacggcgcg cgagccattt
     1321 ctgatgcctg aataccgctg aaacgaaaag gcccggcgtc tgccgggcct gtgctgcgct
     1381 ctggctgtcg cttactggac gacctgcgcg ccgcccgcga agacgatctc gtcgaagacg
     1441 cgattaagcg atttggcgtt gaagttgcgc atcgtcgctc ccggcgccca ggcgatcttg
     1501 tcgccggcct tgacccgcat ctcgggaacg gcaatccaag tcgtcttgcc gctctggtcg
     1561 acttcgaggt aggaataacc ggccgccgtg gtcgcggtct tgacggtgcc ggagttggcg
     1621 ccaccggtaa tcggcatctg gccgccgccg cccatgggcg ggtggcccgc catcggggga
     1681 tggccggcca ttgcgccgcc ggccggctgc gtgccgtgct gggccatcgg tgccatcgca
     1741 gcgccttcct tgccccagtt gttgacgaac agtacctgtt cgaatgtgcg gttaagcgct
     1801 ttcgaattga agttgttcat gatcgcgtag tcgccccact gcaccttgtc gcccactttg
     1861 gcgtcgatgt ggccaccggc gacccagagc ttctgcgcac cggtgtcgac ttcggcgtag
     1921 gtatagatac ctgcctgctc catggacagg atggtgccgg cgttcgggct ggcgctcgcc
     1981 gacttggcga acaggtcggt ggtcggcgca gcgggcgctg ccgactgggc agcgggcttc
     2041 gcgggctctt cgctcttggg cgagcaggcg gcgacggcgg ccgctgcgag gagggtgata
     2101 aatacgttgc ggttcacgag gcgatctcca gactggggcc ggcacacaat gcggcggcgc
     2161 aaaatgctag cacggtcgcg ccgttggcgt ttgaacgggt gttcgagcgg gcctcacttc
     2221 aggcggcgca gcacgttgtg ccgcagtgcc tcggcctggt tggggtcggg cagctgcttg
     2281 gagagctcgg ccagcgccac gccgaggttg gtgatgccgg cttcccacag ctcttcgcag
     2341 agcatgccgg cgatcgggcc gccgatttcg gtcagttctt cctcgatgat tgccctggct
     2401 tcgggcgtca gtacggcacc gcttcccgac ggcgtcgatg cccgctcggt tgccgtggtc
     2461 gcggcgccgg cggcgagcag cttgagcagt tccgcagtcg gcggcaagga cagcactgcc
     2521 ccgcggtggc ggccgggcgc gaagcgggcg gtgccgactt ccatcttctg gtcgcggatc
     2581 gcggcgactg cctcgatgcc ctcgacgttc tggaaggaaa ggaaaacgat ctcgccgttg
     2641 gccagaccaa actgggccag gcgcccgttg atcgtggtcg cgaacagcgt gccgctgttc
     2701 ttgttgtgat gcagccggct cagctcttcg acgaggaagg gcaggaaggg ctggtgggcg
     2761 gtggcggcca tcaaagcacc ccacgcgaca ggtaccgcat tggccgtggg ctatcggtgg
     2821 cgatcatggt gaccgtcatg ccttcttgcg caggcgatcg a
//
LOCUS       DATDAA010000429         2254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_520414,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000429 DATDAA010000000
VERSION     DATDAA010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520414"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VIS73_12865"
     CDS             complement(<1..460)
                     /locus_tag="VIS73_12865"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5764092.1"
                     /translation="MVEPASPRIYRLSDAELAAISAHGITRRYPKNTIIVSEGDSTDG
                     LFIVLEGRVKVFVSDEDGHEVVLGTQGPGEYFGEMALLDAGPRSASVMTLEPSQMIVV
                     SRDNFRGFVEGNPAFAFSLIAKLIARVRALTENVKSLALMDVYGRLARLLL"
     gene            complement(469..1926)
                     /locus_tag="VIS73_12870"
     CDS             complement(469..1926)
                     /locus_tag="VIS73_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEY5764093.1"
                     /translation="MGKVTGFLEHQRLAEASEPVEERKRHYREFVLHLTDDEAKVQGA
                     RCMDCGTPFCMSGCPVNNIIPDFNDLVFRQDWRQAIATLHSTNNFPEFTGRVCPAPCE
                     EACVLRINNDPVGIKSIEHAIIDKAWEAGWVAPQPAAHKTGRRVAVVGSGPAGLACAQ
                     QLARAGHEVVVFEKNDRPGGLLRYGIPDFKLEKWLIDRRLEQLQAEGVSIRTNHMVGK
                     APVGAGNAEAVALDPVTITDEFDAVVLAGGAEQPRDLPIPGRTLEGIHFAMQFLPLQN
                     RRVAGDKGVADLWASDKHVIVIGGGDTGSDCVGTSNRHGARSVTQFELLPMPPDPERN
                     PVWPYWPTRLRTSSSHEEGVSRDWSVATKEFIGENGRVKALKAVRLEWKDGRMQEVPG
                     SEFTLPADLVLLAMGFVSPVQTMLEAFGVEKDARGNAQATTEGEGCYATSVPKVFAAG
                     DLRRGQSLVVWAIREGRQCARAVDEFLMGSSELPR"
     gene            complement(2039..>2254)
                     /locus_tag="VIS73_12875"
     CDS             complement(2039..>2254)
                     /locus_tag="VIS73_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764094.1"
                     /translation="KLSRDLWHLGEADEVILRRMVENQARYTASRRAQALLDNWPEVR
                     TKFVKVFPKEYRRALGELAEAHRKAAA"
BASE COUNT          338 a          806 c          722 g          388 t
ORIGIN      
        1 cgagcagcag ccgcgccagc cgtccgtaga catccatcag cgccagactc ttgacgttct
       61 cggtaagcgc gcgcacgcgc gcgatgagct tggcgatcag gctgaaggcg aaggccgggt
      121 tgccttcgac gaatccgcgg aaattgtccc tcgacacgac gatcatctgg ctcggctcga
      181 gcgtcatcac cgacgcggag cgcggcccgg cgtcgagcag cgccatctcg ccgaaatatt
      241 cccccggacc ctgggtgccg agcaccacct cgtgcccatc ctcgtcgctg acgaagacct
      301 tcacccgccc ttcgaggacg atgaacaggc cgtcggtgct gtcgccctcg ctgacgatga
      361 tggtgttctt gggatacctg cgcgtgatgc catgcgcgct gatcgccgcg agctcggcgt
      421 cgctgagacg gtagatacgc ggactggctg gctcgaccac ggcggctttc agcgcggcag
      481 ttcgctcgat cccatgagga attcgtcgac cgcccgggcg cactgccggc cttcgcggat
      541 cgcccacacg acgagcgact gaccgcgccg caggtccccc gcggcaaaca ccttgggcac
      601 cgaggtggcg taacagcctt cgccttcggt cgttgcctgc gcattgccgc gcgcgtcctt
      661 ctccacgccg aaggcctcga gcatcgtctg caccggggag acgaacccca tcgccagcag
      721 caccagatcc gcgggcagcg tgaattccga gcccggaacc tcctgcatcc tgccgtcctt
      781 ccattccagc cgtacggcct tgagcgcctt gacgcgcccg ttctcgccga tgaattcctt
      841 ggtcgcgacc gaccagtcgc gtgatacgcc ttcctcgtgc gaagaggacg tgcgcagccg
      901 cgtcggccag tagggccaga ccggattgcg ttcagggtcc ggcggcatcg gcagcagctc
      961 gaactgggtg accgaccgcg cgccatgccg gttcgacgtg ccgacacagt ccgagcccgt
     1021 atcgccgcca ccgatgacga tgacgtgctt gtcgctcgcc cacaggtcgg cgacgccctt
     1081 gtcgccggcg acacgccggt tctgcagcgg caggaactgc atcgcgaaat gaatgccctc
     1141 gagcgtgcgc cccgggatcg gcaggtcgcg cggctgttcc gcgccgccgg cgagcaccac
     1201 ggcgtcgaat tcgtcggtga tcgttaccgg gtcgagcgca accgcctcgg cgttgcccgc
     1261 gccgaccggc gccttgccga ccatgtggtt ggtccggatc gacacgccct cggcctgcag
     1321 ctgctcgagg cggcggtcga tcagccattt ttccagcttg aaatcgggga tgccatagcg
     1381 cagcagaccg cccggccggt cgttcttctc gaacacgacg acctcgtgcc ccgcgcgcgc
     1441 cagctgctgc gcgcaggcca ggcccgcagg gcccgagccg acgacggcga cgcgccggcc
     1501 ggtcttgtgc gccgccggct gcggcgcgac ccagcccgct tcccaggcct tgtcgatgat
     1561 cgcgtgctcg atcgacttga tgccgaccgg atcgttattg atgcgcagca cgcaggcctc
     1621 ctcgcagggc gccgggcaga cgcgtccggt gaactcggga aagttgttcg tcgagtgcag
     1681 cgtggcgatg gcctgacgcc agtcctgccg gaacaccagg tcgttgaagt cgggaatgat
     1741 gttgttcacc gggcagcccg acatgcagaa cggcgtgccg cagtccatgc agcgcgcgcc
     1801 ctggaccttc gcctcgtcgt ccgtcagatg caggacgaac tcgcggtagt gcctcttgcg
     1861 ctcctcgacc ggctcggagg cctcggcgag acgttgatgc tcgagaaatc cggtgacctt
     1921 acccatcgtt tgcttccgct cctgtgaccc ttgcgctgcg gggccttgat cgtgtcgctg
     1981 ccgggtgcgg gggacggcta ccacccgccc ccaaccggac ccggccctca cttttcggct
     2041 aggccgcggc cttgcggtgc gcctcggcga gctcgccgag cgcgcgccgg tattccttcg
     2101 gaaagacctt gacgaacttg gtccgcacct cgggccagtt gtcgagcagc gcctgcgccc
     2161 gccggctggc cgtgtagcgc gcctggttct cgaccatgcg ccgcaggatc acttcgtctg
     2221 cttcgcccag atgccacaga tcgcgcgaca gctt
//
LOCUS       DATDAA010000430         3366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_171528,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000430 DATDAA010000000
VERSION     DATDAA010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171528"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VIS73_12880"
     CDS             <1..737
                     /locus_tag="VIS73_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5764095.1"
                     /translation="GIAKACHREGAQLAFTYQTERFADRLAKFAGEFDSKLMFPCDVA
                     DDAQIEALFASLGKEWDGLDGLVHSIAYAPTEAIEGDFLEAISRDAFRIAHDISSYSF
                     AGMAKAARPMLLKGNKASVLCLSYLGAVRTMPNYNVMGLAKASLEAATRYMAVTLGPQ
                     GVRANSISAGPIKTLAASGIGSFSKLLAYNSHHSPLRRNVTIDEVGNAAAFLLSDLAS
                     GITGEIMYVDGGFNTTALGNSAPLPG"
     gene            complement(823..2715)
                     /locus_tag="VIS73_12885"
     CDS             complement(823..2715)
                     /locus_tag="VIS73_12885"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY5764096.1"
                     /translation="MFESIRSSKWFVQIFLFLIAISFALWGVESYLRDMGNEDTIATV
                     GGTKITQAEFAQALREQQDQLAEAMGASYTPDMLNTPEARRGLVAQLVNQRLLMLDAR
                     ERNIVVSDAMLAEVIKGIPSLMENGQFSNDRYEAFARARGYSVAGFESQLRRDLLIQQ
                     ALSAVGESAVGSEVGYKATYALLAEKREVAEVRLKPDSFRSGVKIDDEAVKAYYDANP
                     KRFEQAEQVRASYVTLTPEAIEKQVSVKDEDIRSWYEANKARYSQPEERRASHILIAA
                     DKDAAADVKAEARKRVEKLLADVRARPGEFARIARENSDDPGSAGNGGDLGYFARGAM
                     EPSFEDAAFALPDGGLSDIVVSDFGFHIIKVTGLNPGREKPFAEVKSEIERELRDQAV
                     QRRFAEAAEQFANVAYEQSDSLEPLVEQFKLKPVETGWVSRRNPVSAPGLSPKVVAAL
                     FTDDAVKEKRNTEAVEVAPRTLVVARVLEHRPAARLPLAEVEKDIRDFLIDEAAAKKT
                     AEAGEARLAELRAGKSASFPKPITISRLDHAGLPPVAARAVLDAPADKLPAYVGVAHP
                     DGGYGLYRISAVEAAPADDSQVSQALRTELVNLQLQQELQAYLASLRKRYEVEVNART
                     FEAARS"
     gene            complement(2781..2857)
                     /locus_tag="VIS73_12890"
     tRNA            complement(2781..2857)
                     /locus_tag="VIS73_12890"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2821..2823),aa:Asp,seq:gtc)
     gene            2993..>3366
                     /locus_tag="VIS73_12895"
     CDS             2993..>3366
                     /locus_tag="VIS73_12895"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEY5764097.1"
                     /translation="MARIHPDGWRELAASGAAERELQTLSQLAAGLSDRYAVYHGVHW
                     TRATAHYSVVGEIDFVIVGPSGKILLVEQKSGFLSEGEEGLCKPYANKSKNVAAQMAR
                     NTRNFISRLRGACPDASVEVEAL"
BASE COUNT          580 a         1156 c         1045 g          585 t
ORIGIN      
        1 atggcattgc caaggcctgt caccgtgagg gtgcgcaact cgccttcacc taccagaccg
       61 agcgtttcgc cgaccggctc gcgaaattcg cgggtgaatt cgacagcaag ctgatgttcc
      121 cttgcgatgt cgccgacgac gcgcagattg aggcgctgtt cgcctcgctc ggcaaggaat
      181 gggacggcct cgacggcctg gtccattcga tcgcctacgc gccgaccgag gcgatcgagg
      241 gcgactttct cgaagcgatc agccgcgatg ccttccgcat cgcccacgac atctcctcgt
      301 acagcttcgc cggcatggcc aaggcggcgc ggccgatgct gctcaagggc aacaaggcgt
      361 cggtgctctg cctttcctat cttggcgccg tgcgcacgat gcctaactac aacgtgatgg
      421 gtctggccaa ggccagcctc gaagcggcga cgcgctacat ggccgtcacc ctaggcccgc
      481 agggcgtgcg cgccaacagc atctcggccg gcccgatcaa gacgctcgca gcttcgggca
      541 tcggtagctt ctccaagctg ctcgcctaca actcgcacca ttcgccgctg cgccgcaacg
      601 tgacgatcga cgaggtcggc aatgccgccg ccttcctgct gtccgacctc gcttcgggca
      661 tcaccggcga gatcatgtac gtcgatggcg gcttcaacac cacggcactc ggcaactcgg
      721 cgccgctgcc cggctagcac tgcgggccgc tgcacgccac aagcaaagac gccggcaatt
      781 gccggcgtcc tgctttgtgc ttctggaacc tcggcgccgc gcttagctcc gcgccgcttc
      841 gaaggtccgc gcgttgactt caacctcgta gcgcttgcgc aggcttgcca gatacgcctg
      901 cagttcctgt tgcagttgga ggttgaccag ttcggtgcgt agcgcctgcg acacctgaga
      961 gtcgtctgcc ggcgccgctt cgacggccga tatgcggtag aggccgtaac cgccgtcggg
     1021 gtgcgccacg ccaacgtagg ccggcagctt gtcggccggc gcatcgagca ccgcccgtgc
     1081 ggccaccggc ggcaggccag cgtgatcgag acgcgagatc gtgatcggct tggggaagct
     1141 cgccgacttg ccggcacgca attcggcaag gcgcgcctca ccggcttcgg cagtcttctt
     1201 cgctgccgct tcgtcgatca ggaagtcacg gatgtccttc tctacttcgg ccaggggcag
     1261 gcgggcggcc gggcgatgct cgagcacgcg ggcaacgacc agcgtgcgcg gcgcgacctc
     1321 gacggcttcg gtattgcgct tttccttgac cgcatcgtcg gtaaacagcg cggcaacaac
     1381 cttgggcgac aaccccggtg ccgacaccgg attgcggcgc gacacccagc cggtctcgac
     1441 cggcttcagc ttaaattgct cgaccagcgg ctccaggctg tccgactgct cgtaggcgac
     1501 gttggcgaac tgctcggccg cctcggcaaa gcgccgctgc acagcctgat cgcgcagctc
     1561 gcgctcgatc tcggacttca cttcggcgaa cggcttttcg cggccgggat tgaggccggt
     1621 gaccttgatg atgtgaaaac cgaagtcgga gacgacgatg tcggacaagc cgccatcggg
     1681 caaggcgaag gccgcgtcct cgaagctcgg ttccatcgcg ccgcgcgcga agtagccaag
     1741 gtcgccgccg ttgccggccg agcccgggtc atcggaattc tcgcgggcaa tccgcgcgaa
     1801 ctcgccgggt cgtgcgcgaa cgtccgccag cagcttctcg actcgcttgc gcgcctcagc
     1861 cttgacgtcg gccgccgcat ccttgtcggc ggcgatcagg atatgactgg cgcggcgctc
     1921 ctcgggttgc gagtaacgcg ccttgttggc ctcgtaccag gaacggatgt cttcgtcctt
     1981 gacgctgacc tgcttctcga tagcttccgg cgtcaacgtc acgtaactcg cgcggacctg
     2041 ctcggcctgc tcgaatcgct tcggattcgc gtcgtagtag gccttgaccg cttcatcgtc
     2101 gatcttgacg cccgagcgaa agctgtccgg cttgagacgg acctcggcga cctcgcgctt
     2161 ctcggcgagc aaggcgtagg tcgccttgta accgacttcg ctgccgacgg ccgactcgcc
     2221 gaccgccgaa agcgcctgct ggatcagcag gtcgcgccgc agctgcgact cgaagccggc
     2281 caccgagtag ccgcgcgcgc gcgcaaaggc ttcgtaacgg tcattggaga actgcccgtt
     2341 ttccatcagc gagggaattc ccttgatcac ttcggcgagc atcgcgtcgc tgacgacgat
     2401 gttgcgctcg cgggcatcaa gcatcagcag gcgctggttg accaattgcg ccaccagtcc
     2461 ccgccgcgcc tcgggcgtgt tcagcatatc gggcgtatag ctcgcgccca ttgcctcggc
     2521 cagttggtcc tgctgctcgc gcagcgcctg ggcgaactca gcctgggtga tcttggtgcc
     2581 accgaccgtg gcgatcgtat cctcgttgcc catgtcacgc aggtaggact cgactcccca
     2641 gagggcgaag gagatggcaa taaggaagag gaaaatctgg acaaaccact tgctgctgcg
     2701 aatcgactcg aacatggcgg ctttatgacc ccgaaatcgg caagaaacaa aaaacaagcg
     2761 cgctctctgg cgcgcgatgc tggcggagcg gacgggactc gaacccgcga cccccggcgt
     2821 gacaggccgg tattctaacc aactgaacta ccgctcctcg cctgccatcc cggcctctgg
     2881 cggcaccggc acggcggcga agtatagcac cgccgcatgc gccgccattc caccgttgat
     2941 gcgaaaatgc gcatcccgcg gcctttccgc cgccactgcg cggcaaccga caatggcaag
     3001 gattcatccc gacggctggc gcgagctggc cgccagcggc gccgccgagc gcgaactgca
     3061 aacgctgtcg cagctcgccg ccggcctctc agaccgctac gccgtctacc acggcgtcca
     3121 ttggacgcgt gcgaccgcgc attacagcgt cgtcggcgaa atcgatttcg tgatcgtcgg
     3181 gccgagcggc aagatcctgc tcgtcgaaca gaaaagcggc ttcctcagcg aaggcgaaga
     3241 aggcctctgc aagccctacg ccaacaagtc gaagaatgtc gcggcgcaga tggcgcgcaa
     3301 cacccgcaac ttcatcagcc gcctgcgcgg cgcctgcccc gacgccagcg tcgaggtcga
     3361 ggccct
//
LOCUS       DATDAA010000431        10871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_311674,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000431 DATDAA010000000
VERSION     DATDAA010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 10871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10871
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311674"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(455..829)
                     /locus_tag="VIS73_12900"
     CDS             complement(455..829)
                     /locus_tag="VIS73_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764098.1"
                     /translation="MIDTAAIVGLATLVAVIVFFWKRFGIGSGHALGNRIAAHIGMPR
                     NLFYLFLDNGAKGSSRDLLAALEQANLDLDQASVELAPSLARGIERLEERFGTQGVYE
                     KVKPSVARLMSEFERKQSASEN"
     gene            complement(992..1915)
                     /locus_tag="VIS73_12905"
     CDS             complement(992..1915)
                     /locus_tag="VIS73_12905"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY5764099.1"
                     /translation="MTTLSIHHQTTYRYRESLEFGPHRLMLRPRESRNLRLIGHELSF
                     TPAATVTWAHDVSGNAVATAVFQEKSDTLVIDSLAELTLDVDRWPVFDIAASAANYPF
                     LLSEDDMTDLGALRLQSYFDSTGQLREWAQGFVAGNPTGTLALLRDICSGVAARVSYE
                     VREEDDAQSPEQTLARSRGSCRDFAVLFVDAVRSLGFGARIVSGYLFDPDQDALGSSG
                     CGSTHAWAEVYVPGAGWITFDPTNRSIGGYNLIPVAVARHIRQTMPVSGSFVGDSKAF
                     ERMEVRVDVAPTVHRRGAPVTPARAYPDVGA"
     gene            complement(2139..4124)
                     /gene="accC"
                     /locus_tag="VIS73_12910"
     CDS             complement(2139..4124)
                     /gene="accC"
                     /locus_tag="VIS73_12910"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY5764100.1"
                     /translation="MFKKILIANRGEIACRVIKTAKKMGIATVAVHSEADKDALFVEM
                     ADEAVHIGPAASKESYLVIDKIIAACKKTGAEAVHPGYGFLSENMEFSKRLEENGIVF
                     IGPKHYSVGAMGDKIASKKLALEAKVNTIPGYNDVIRDGKHAVEIAKGIGYPVMIKAS
                     AGGGGKGLRVAFNDKEAAEGFEACRNEAATAFGDDRIFIEKFVEGPHHIEIQIIADSH
                     GNTCYLWERECSIQRRHQKVIEEAPSPFIDDKVRKAMGEQAVALAKAVKYQSAGTVEF
                     VVGKDKSFYFLEMNTRLQVEHPVTEMITGLDLVELMIRVAAGEKLPFKQEDVKRDGWA
                     MECRINAEDPFRGFLPSTGRLVKFMPPTDADGVRVDTGVYEGGEISMYYDSMIAKLIC
                     HGGTREQAIKRMRDALNAFVIRGVSSNIPFQAALMQHPVFNSGIFTTAFIADHYPKGF
                     VAADVPHDDPGLLAAVAAFARRRFIDRARLTSGQMPGHARKVGSEWDVAIEGTHYSIT
                     VDPIDGGYAVVHGNDRYELVSDWSFGDLIFHGTCNGAPVCMQLERNGLRYRITHFGKQ
                     VDAMVLTRTASRLLSRMPEKIPPDLSRFLLSPMPGLLREVSVKVGQAVSVGEKLAVIE
                     AMKMENILRAEKDCVVKTIAAEPGVSLMVDQVIIEFE"
     gene            complement(4144..5676)
                     /locus_tag="VIS73_12915"
     CDS             complement(4144..5676)
                     /locus_tag="VIS73_12915"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEY5764101.1"
                     /translation="MHDIIRQLEEKRAAAVLGGGQKRIDSQHKKGKLTARERIELLLD
                     SDSFEEWDMFKEHRCTDFGMADEKVPGDGVVIGCGTVNGRLVFVFSQDFTVLGGSLSE
                     THAEKICKVMDHAIKVGAPIIGLNDSGGARIQEGVASLGGYADVFQRNVMASGVIPQI
                     SMIMGPCAGGAVYSPSMTDFIFMVKDSSYMFVTGPEVVKTVTHEDVTAEELGGAVTHT
                     SRSGVADLAFDNDVEALMMLRRLISFLPASNREKPPVIPLQDPAERMDYSLDTLVPDN
                     TNKPYDIKELIVKTVDDGDFFELQPDYAKNIVIGFGRMEGSTVGIVANQPLVLAGCLD
                     IKSSIKAARFVRFCDAFNIPVVTFVDVPGFMPGTAQEYGGIIKHGAKLLYAYAECTVP
                     KITVITRKAYGGAYDVMSSKHLRGDVNFAWPSAEIAVMGPKGAVEIIFREEKGDPEKL
                     AGREAEYKAKFANPFVAGARGFIDDVIMPHETRKRICRSLAMLRGKKLENPWRKHGNI
                     PL"
     gene            complement(5715..6731)
                     /gene="meaB"
                     /locus_tag="VIS73_12920"
     CDS             complement(5715..6731)
                     /gene="meaB"
                     /locus_tag="VIS73_12920"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEY5764102.1"
                     /translation="MTDLSATGATLPANDRRLADGVAAGERRSLAKAITLVESTRADH
                     QARAQDLLNALLPHSGNAIRLGISGSPGAGKSTFIEALGMWLIERGHKVAVLAVDPSS
                     SVSGGSILGDKTRMERLSMRTEAFIRPSPSAGSLGGVAEKTREAMLLCEAAGFDVVII
                     ETVGVGQSEITVAGMVDAFCLVQLPNAGDDLQAIKKGIVELADVVLINKADIDPRSTA
                     AARSQWASALTMLRPATPHWKPPVLTISALREEGIAAFWEAIEQYRTALNAAGALAEK
                     RRRQALDWMWALVDAGLRQRFRDHPGVREALARSLEAVASGQLTPAAAAHRLLDAQQS
                     PINT"
     gene            complement(6760..8922)
                     /gene="scpA"
                     /locus_tag="VIS73_12925"
     CDS             complement(6760..8922)
                     /gene="scpA"
                     /locus_tag="VIS73_12925"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEY5764103.1"
                     /translation="MADTKPTLPDSSQLDAWTKAAAKSAPGGQVEALNWITPEGLTVK
                     PLYTKRDVADLPHADTLPGFAPYLRGPQATMYAARPWTIRQYAGFSTAEASNAFYRKA
                     LAAGGQGVSVAFDLATHRGYDSDNPRVVGDVGKAGVAIDSVEDMKILFDGIPLDKISV
                     SMTMNGAVLPILAGYIVAAEEQGVKPEQLSGTIQNDILKEFMVRNTYIYPPTPSMKII
                     ADIFGYTASHMPKFNSISISGYHIQEAGGNQAIELAFTLADGMEYVRTGVASGMDVDT
                     FAGRLSFFWGVGMNFYLEIAKMRAARLLWHRIMSNFGAKSPKSLMLRTHSQTSGWSLT
                     EQDPYNNVVRTTIEAMAAVFGGTQSLHTNALDEAIALPTDFSSRIARNTQLIIQEETH
                     ITNVVDPWAGSYMMEKLTQDMADKAWAIIEEIEAMGGMTKAVESGWAKLQIETCAADK
                     QARIDSGKDVIVGVNKYKLAKEDPIEILDIDNHAVRESQVTRLKQIRKTRDAAAVDVA
                     LKALTEAAKTGKGNLLDLAVKAMRLRATVGEISDALEAVFGRYRAMHQTVSGVYGGVV
                     AEADSWKTLKADVEAFAKEEGRRPRIMIAKLGQDGHDRGAKVVASAFADLGFDIDIGP
                     LFQTPEEAARQAIENDVHAIGVSTLAAGHKTLVPQLIGALKHAGADDVIVFAGGVIPA
                     QDYAFLFESGAKAVFGPGTRIEDSAKTVLDEIRKARRA"
     gene            9149..9823
                     /locus_tag="VIS73_12930"
     CDS             9149..9823
                     /locus_tag="VIS73_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5764104.1"
                     /translation="MTRIAPTALYQEVAERLRQRIYAHEIEPGAWIDEQAIATQYGIS
                     RTPLREALKVLASEGLVTLKPRRGCYVTELSEQDLNDIFPLLAVLEGRCALEATRKAG
                     ASDLKRLAEIHERLEQAAAKGQTDRFFEANQQFHATIQTLAGNRWLTQMIADLRKVVK
                     LTRVLSLSLKGRLEQSLLEHRVIIKAMTAGEAEVAEKAMAAHLESAREALIRDLITAR
                     KSPRAA"
     gene            9891..10631
                     /locus_tag="VIS73_12935"
     CDS             9891..10631
                     /locus_tag="VIS73_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HEY5764105.1"
                     /translation="MSKKIAYVTGGMGGIGTSICQRLAKDGFTVVAGCGPNSPRKDKW
                     LAEQKALGFDFVASEGNVADWDSTVAAFAKVKAEVGEIDVLVNNAGITKDGVFRKMTP
                     ADWKAVIDTNLNSMFNVTKQVIDGMMDRGWGRVINISSMNGQKGQFGQTNYSTAKAGM
                     RGFTMALAQEVAGKGVTVNTVSPGYIGTDMVKAIREDVLQKIVGTIPVKRLGEPAEIA
                     SIIAWLASEESGFATGADFSLNGGNYMG"
BASE COUNT         1926 a         3409 c         3502 g         2034 t
ORIGIN      
        1 ctgcgctgct taccggtcgt ccgcgtagag tccatcggtt gaccgttttg ggtcgggtcc
       61 ggtcctacga ccctcgcgca gaccacccga aagccgtcgc tgagttcgac cgtcagctac
      121 cagactgcca gcggtcgctc aaacggctcc gcgtgaccga ccggaatggc cgacgagtac
      181 tcagccagca gtcgcaccga gcgactgctc cgcccagaat cctgccgtta gacctccggt
      241 acggcgaatg tctcttcggg gtcgaaagtg tgagtttgct ggaccagaca gcggccgttc
      301 agcgggattt tgagccgatg gtcagcttgg cgactcgatg ctgtccgacg cggcaggtct
      361 cagcctgaag cacatgctcg aatggcggcg cggtcgtgat ctgcagacga cagcgtggcg
      421 cgccggcgaa cccggtgtcg ggcaccgcag ggcgtcaatt ctccgaagcg gattgctttc
      481 gttcgaactc cgacatcagc ctcgccacgc tgggctttac cttttcgtac accccctggg
      541 tgccaaagcg ttcctcgagg cgttcgatcc ctctggcgag tgacggggca agttcgacac
      601 tggcctgatc cagatccagg ttcgcctgtt caagcgctgc caacaggtca cgtgacgagc
      661 ctttggcgcc attgtcgagg aacaggtaga acagatttct tggcatgcca atgtgcgctg
      721 cgatgcgatt tcccaaggcg tggccggagc cgattccaaa tcgcttccag aagaagacaa
      781 tcacggcaac gagggtagca agtccaacga ttgcggctgt atcgatcaag gttgtgcgcc
      841 ctcccggcgt ggttcagatg aagtgcctcg actgcaatgg atgcgaggcg tgatgtgatg
      901 cagccctggg cgttgacgca tgctcagcgg ccgggaatca gacgatgcca aacagaatgc
      961 cgcaagcgcc cacggcctgt gacgcgtccg cttacgcacc gacatccgga tatgcgcgcg
     1021 ccggtgtaac gggagccccg cgccgatgga cggtgggagc gacgtctacc cgcacctcca
     1081 tccgttcgaa cgccttgcta tcgcccacaa agctgccgct gaccggcatg gtctggcgga
     1141 tatggcgggc aacggcgacc gggatgagat tgtagccgcc aatgctgcgg ttggttggat
     1201 cgaaggtgat ccaacccgcg ccgggcacat agacctccgc ccaggcatgg gtcgacccgc
     1261 aaccgcttga gccgagcgca tcctgatccg gatcgaacag gtatcccgag acaatgcgcg
     1321 caccgaaacc gaggctgcgc accgcatcta caaacagtac cgcgaaatca cggcatgagc
     1381 cgcgactacg ggcgagcgtc tgctcgggcg actgcgcgtc atcttcttcg cgcacttcgt
     1441 agctgacccg cgcggcaacg ccggaacaga tgtccctgag cagggcgagt gtcccggtcg
     1501 ggtttccggc aacgaaacct tgcgcccact cgcggagctg gccggtggaa tcgaaataac
     1561 tctgcagccg caaggcgccg aggtcggtca tgtcgtcctc gctcagcagg aacgggtaat
     1621 ttgcagccga ggcagcgata tcgaagacgg gccaccggtc gacgtcgagc gtgagttcgg
     1681 caaggctgtc gatgaccagc gtgtctgact tttcctgaaa cacggccgtc gccacggcgt
     1741 tgccggaaac gtcgtgcgcc caggtcaccg tcgccgccgg cgtaaacgac agctcatgac
     1801 cgataagccg aaggttgcgg ctctcacggg gacgaagcat caggcgatgg gggccgaatt
     1861 caagcgattc ccgatatcga taggtcgtct ggtgatggat tgaaagcgtg gtcaacgggc
     1921 tggctccttg cgagccccat catagaccct cggtggccaa ttcctggcga acggcgagcg
     1981 cgggcatcaa acccacccaa tctccactcg gcgtcggggc gcaaaccggc gatgggggcg
     2041 gccttcattt gcttccgagg tcgatgtctg gcagcgctgt cgcccaaaga aaaagccccg
     2101 cactcggcgg ggcttgtcgt atcaggcgct tacccgaatc actcgaactc gatgatcacc
     2161 tgatcaacca tgaggctgac gccaggctcg gctgcgatcg tcttgaccac gcagtccttt
     2221 tcggcgcgga ggatattctc catcttcatc gcttcgatga cggcgagctt ctcgccgacg
     2281 ctgactgcct gaccgacctt gaccgacact tcacgcagca gacccggcat cggcgagagc
     2341 aggaagcgcg acaggtcggg cgggatcttc tcgggcatcc gagagagcag gcgcgaggcg
     2401 gtgcgcgtga gcaccatcgc gtcgacctgc ttgccgaagt gggtaatccg gtagcgcagg
     2461 ccgttgcgct cgagctgcat gcacaccggg gcgccgttgc aggtgccgtg gaagatcaga
     2521 tcgccgaagc tccagtccga aaccaactcg tagcgatcgt tgccgtggac gacggcgtag
     2581 ccgccgtcga ttggatcgac ggtgatcgag tagtgcgtgc cttcgatggc gacatcccat
     2641 tcgctgccga ccttgcgcgc gtggccgggc atctggccgc tggtcagtcg ggcgcggtcg
     2701 atgaagcggc gacgcgcgaa ggcggcgacc gcggcgagca gaccggggtc gtcatgcggc
     2761 acgtcggcgg cgacgaagcc cttggggtag tggtcggcga tgaaggccgt ggtgaagatg
     2821 cccgagttga acaccgggtg ctgcatcaag gccgcctgga acgggatgtt ggagctgacg
     2881 ccgcggatga cgaaggcgtt aagggcgtcg cgcatgcgct tgatcgcctg ctcgcgggtg
     2941 ccgccatgac agatcagctt ggcgatcatc gagtcgtagt acatcgagat ctcgccgcct
     3001 tcgtagacgc cggtgtcgac gcgcacaccg tcggcatcgg tcggcggcat gaacttcacc
     3061 agccgtccgg tcgagggcag aaagccacgg aaggggtctt cggcgttgat ccggcattcc
     3121 atcgcccagc catcgcgctt gacgtcttcc tgcttgaacg gcagcttttc gccggcggcg
     3181 acgcggatca tcaattccac gaggtcgagg ccggtgatca tttcggtgac cgggtgctcg
     3241 acttgcagtc gggtgttcat ttcgaggaag tagaaggact tgtccttgcc gacgacgaac
     3301 tcgacggtgc cggccgactg atacttgact gccttggcca atgccaccgc ctgctcgccc
     3361 atcgccttgc gcaccttgtc gtcgatgaac ggcgacggcg cttcctcgat caccttctgg
     3421 tggcgacgct ggatcgaaca ctcgcgctcc cacaggtagc acgtgttgcc gtggctgtcg
     3481 gcgatgatct ggatttcaat gtggtgcggg ccttcgacga atttctcgat gaagatccgg
     3541 tcgtcgccga aggcggttgc cgcttcgttg cggcaggcct cgaagccttc ggctgcttcc
     3601 ttgtcgttga aggccactcg caggcccttg ccgccgccac cggcggaggc cttgatcatc
     3661 accggatagc cgattccctt ggcaatctcg accgcgtgct tgccatcgcg gatgacatcg
     3721 ttgtagccgg gaatggtgtt gaccttggct tcgagcgcca gctttttcga ggcgatcttg
     3781 tcgcccatcg cgccgaccga gtagtgcttg ggcccgatga agacgatgcc gttctcttca
     3841 agtcgcttgg aaaactccat gttctccgag aggaagccgt agcccggatg aacagcctcg
     3901 gcgccggtct tcttgcaggc ggcgatgatc ttgtcgatga cgaggtagga ctccttcgag
     3961 gcagccgggc cgatatgcac ggcctcgtcg gccatctcga cgaagagcgc atccttgtct
     4021 gcctcggaat gcacggcgac cgtcgcgatg cccatcttct tggcggtctt gatgacgcgg
     4081 caggcgattt caccgcggtt ggcaatcagg attttcttaa acattgtctt ttctccccgg
     4141 cgatcagagc ggaatgttgc cgtgcttgcg ccacggattt tcgagcttct tgccacgcag
     4201 catcgccagc gagcggcaga tgcgcttgcg tgtctcgtgc ggcatgatca cgtcgtcgat
     4261 gaagccgcgc gcgccagcga caaaggggtt ggcgaacttg gctttgtact cggcctcacg
     4321 cccggcgagc ttctcggggt ctcccttctc ctcgcggaaa atgatctcga ctgcgccctt
     4381 cggacccatc accgcgattt cggccgacgg ccaggcgaaa ttcacgtcgc cacgcaggtg
     4441 cttcgaactc atgacgtcgt aagcgccgcc gtaagccttg cgggtgatga ccgtgatctt
     4501 gggcaccgtg cactcggcgt acgcatagag tagcttggcg ccatgcttga tgatgccgcc
     4561 gtattcctgc gcggtaccgg gcatgaaacc cggcacgtcg acgaaggtca ccaccgggat
     4621 gttgaatgcg tcgcagaaac gaacgaagcg cgcggccttg atcgaactct tgatgtcgag
     4681 gcagcccgct agcaccagcg gctggttggc gacgatgccg accgtcgagc cttccatccg
     4741 gccgaagccg atcacgatgt tcttggcgta gtcgggctgc agctcgaaga agtcgccgtc
     4801 gtcgacggtc ttcacgatca gctccttgat gtcgtagggc ttgttggtgt tgtcgggcac
     4861 cagcgtgtcg agcgagtaat ccatgcgctc ggcggggtcc tgcagcggta tcaccggcgg
     4921 cttctcgcgg ttggaggcgg gcaggaagct gatcagccgg cgcagcatca tcagcgcctc
     4981 gacgtcgtta tcgaaggcga ggtcggcaac gccgctgcgg ctagtgtggg tcacggcgcc
     5041 acccagttcc tccgcggtca cgtcctcgtg agtcacggtc ttaacgactt cgggaccggt
     5101 gacgaacatg taggagctgt ccttgaccat gaagatgaag tcggtcatcg acggcgagta
     5161 cacggcgccg ccggcgcagg ggcccataat catcgagatc tgcgggatga cgccgctggc
     5221 catcacgttg cgctggaaga cgtcggcgta accgccgagc gaagcgacgc cttcctggat
     5281 ccgcgcgcca cccgaatcgt tgaggccaat gatcggcgca ccgaccttaa tcgcgtggtc
     5341 catcaccttg cagattttct cggcgtgggt ttccgacagc gaaccgccga gtaccgtgaa
     5401 gtcctgcgag aagacaaaga cgaggcgacc gttgacggtg ccgcagccga tgacgacgcc
     5461 gtcgcccggc accttctcgt cggccattcc gaagtcggtg cagcgatgct ccttgaacat
     5521 gtcccactct tcgaaggagt cggaatcgag cagcagctcg atacgttcgc gcgcggtgag
     5581 tttgcccttt ttgtgctggc tgtcgatgcg cttttgtccg ccgccgagta cggccgcggc
     5641 acgcttctcc tccagttgcc ggatgatgtc atgcatggtg ttctccctgg aaacaggtct
     5701 ggatacgaaa tcgttcaggt gttgatcggt gactgctgcg catcgagcag tcggtgtgcc
     5761 gcggcggcgg gcgtgagttg cccgcttgcc acggcctcga gtgatcgggc gagcgcttcg
     5821 cgcacgcccg ggtgatcgcg gaaacgctgg cgcaggccgg catcgaccaa ggcccacatc
     5881 cagtcgagcg cctgccggcg gcgcttttcg gccagtgcgc ctgcggcatt taaggccgtg
     5941 cggtactgct cgatggcctc ccagaaggcg gcaatgcctt cctcgcgcag cgccgagatg
     6001 gtgagcaccg gcggcttcca gtgcggtgtc gccgggcgca gcatggtcag tgcgctggcc
     6061 cactggctgc gagccgcagc ggtcgagcgg gggtcgatgt cggccttgtt gatgagtacg
     6121 acatcggcga gttcgacgat gcccttcttg atcgcctgca gatcgtcgcc ggcgttgggc
     6181 agctggacca ggcagaaggc atcgaccatg ccggcgaccg tgatctcgct ctggccgacc
     6241 ccgacggtct cgatgatcac cacgtcgaag cccgccgcct cgcagagcag catcgcctcg
     6301 cgggtctttt cggcgacgcc gccgagcgaa cccgccgacg ggctcgggcg gatgaatgcc
     6361 tcggtccgca tcgacagccg ttccattcgc gtcttgtcgc cgagaatcga accaccggaa
     6421 accgaggaag acgggtcgac ggcaagtact gcgaccttgt gcccgcgctc aatcagccac
     6481 atgccgagcg cctcgatgaa ggtgctcttg ccggcccccg gagagccaga gatgcccagc
     6541 cggatcgcgt tgcccgagtg cggcagcagc gcgttgagca ggtcctgcgc gcgcgcctga
     6601 tgatcggcgc gggtgctctc gaccagcgtg atcgccttcg ccagtgagcg ccgttccccg
     6661 gcagccacac cgtcggcgag ccggcggtcg ttggctggca gggtggcacc cgtagccgag
     6721 aggtcagtca tggcggtcac gtgcgcgctg gcgcggcgat caggcgcggc gcgccttgcg
     6781 gatctcatcg agcacggtct tcgccgaatc ttcgattctc gtacccgggc cgaagacggc
     6841 cttggccccc gattcgaaga ggaaggcgta gtcctgcgct ggaatcacgc caccggcgaa
     6901 tacgatgacg tcgtcggctc cggcgtgctt gagcgcgccg atcagttgcg gaaccagcgt
     6961 cttgtggccg gctgccaggg tcgagacgcc gatcgcgtgg acatcgttct cgatcgcctg
     7021 ccgcgctgcc tcttctggcg tctggaaaag cgggccgatg tcgatgtcga aaccgagatc
     7081 ggcgaaggcc gaggcgacga ccttggcacc gcggtcgtgg ccgtcctgac cgagcttggc
     7141 gatcatgatc cgcggccggc ggccctcttc cttggcaaag gcttcgacat cggccttcag
     7201 ggtcttccag ctatcggctt cggccacgac gcctccatag acacccgaca cggtttgatg
     7261 catcgcgcgg tagcggccaa acacggcctc cagcgcgtcc gaaatttcgc cgacggtggc
     7321 gcgcaggcgc atggccttga ccgccaggtc gagcaggttt cccttgccgg tcttcgccgc
     7381 ctcggtgagc gccttcagcg ctacgtcgac tgccgccgca tcgcgcgtct tgcggatttg
     7441 cttgagccgc gtcacttgcg attcgcgtac ggcatggttg tcgatatcga ggatctcgat
     7501 cgggtcttct ttggccagct tgtacttgtt gacgccgacg atgacatcct tgcccgagtc
     7561 gatgcgcgcc tgcttgtcgg cagcgcaggt ctcgatctgc agtttggccc agcccgattc
     7621 gacggccttg gtcatgccgc ccatcgcctc gatttcctcg atgatggccc atgccttatc
     7681 ggccatgtcc tgcgtcagct tctccatcat gtaggagccg gcccaggggt cgacgacatt
     7741 ggtgatgtgg gtttcttcct ggatgatcag ctgcgtattg cgggcgatgc gcgaggagaa
     7801 atcggtaggc agcgcgattg cctcgtcgag cgcgttggta tgcagcgact gcgtgccgcc
     7861 gaagaccgcg gccatcgcct cgatggtcgt gcgtaccacg ttgttgtagg ggtcctgctc
     7921 ggtcagcgac cagccggagg tttggctgtg cgtgcgcaac atcagcgact tcgggctctt
     7981 ggcgccgaag ttcgacatga tccgatgcca gagcaggcgc gctgcacgca tcttggcgat
     8041 ctcgagatag aagttcatcc cgacgcccca gaagaaggac agtcggccgg caaaggtatc
     8101 gacgtccatg cccgaagcga cgcctgtgcg cacgtactcc atgccgtcgg cgagggtgaa
     8161 tgccagttcg atcgcctggt taccgccggc ctcctggatg tgataacccg agatcgaaat
     8221 cgagttgaac ttcggcatgt ggctcgccgt gtacccgaat atgtcggcga tgatcttcat
     8281 cgacggtgtc ggcggataga tataggtgtt gcggaccatg aactccttga ggatgtcgtt
     8341 ctggatggtc ccggagagtt gttcgggctt cacgccctgc tcttcggcag caacaatgta
     8401 gccggcgagg atcggcagca ccgcgccgtt catcgtcatc gagacgctga tcttgtcgag
     8461 cggaatgccg tcgaagagga tcttcatgtc ctcgaccgag tcgatagcta cgcctgcctt
     8521 gccaacatcg ccgacaacgc gcggattgtc cgagtcgtag ccgcggtgcg tggcgaggtc
     8581 aaaggccact gagacgccct ggccacctgc ggccagtgcc ttgcggtaga aggcgttgga
     8641 cgcctcggcg gtcgagaagc ctgcgtactg gcggatcgtc cacggccgcg ctgcgtacat
     8701 cgtcgcctgt gggccgcgta ggtagggcgc gaatccgggc agcgtgtcgg cgtgcggcag
     8761 gtcggcgacg tcccgcttcg tatagagcgg cttgacggtc aggccctcgg gcgtgatcca
     8821 gttgagcgcc tcgacctggc cgccgggcgc cgacttggca gccgccttgg tccaggcatc
     8881 gagttgggac gaatcgggca gcgtcggttt ggtgtcggcc atctgggatt cctttcctgc
     8941 gtcgggagag caggcagtca gtttagtaaa gccgcttgca gaagcgacag cccctcacaa
     9001 tgcgccgctt gagccgattc ccgctgacgg gttggacgag cgcgccccct tgcttgacgg
     9061 gcatcagcaa acataatact gtatccataa ttatgaatgc aagcggcgag acgtgcgcca
     9121 gactcgcccg tccgccaaag gacagcagat gacccgaatt gccccgacgg ccctctacca
     9181 ggaggtcgcg gaacgcctga gacagcggat ctacgcgcac gagatcgagc ccggcgcgtg
     9241 gatcgacgaa caggcgatcg ccacccaata cggaatttcg cgcaccccgc tgcgcgaggc
     9301 gctgaaggtg ctcgcctccg aagggctggt cacgctcaag ccacgccgcg gctgctacgt
     9361 caccgagctc tcggaacagg atctcaacga catcttcccg ctgctcgccg ttcttgaagg
     9421 gcgctgcgcg ctggaagcga cgcgcaaggc aggcgccagc gacctcaagc gccttgccga
     9481 aatccacgag cgactcgagc aggcggccgc gaaggggcag accgaccgtt tcttcgaagc
     9541 caaccagcag ttccacgcga ccatccaaac gctcgccggc aaccgctggc tgacgcagat
     9601 gatcgccgac ctgcgcaagg tggtgaagct gacccgcgtg ctttcactct cgctcaaggg
     9661 ccggctcgag cagtcgctgc tagaacaccg cgtgatcatc aaggcgatga ccgccggcga
     9721 ggccgaagtg gccgagaagg cgatggcggc gcatctggaa agcgcccgcg aggcgctgat
     9781 ccgcgacctc atcaccgccc ggaagtcccc acgcgccgcc tgaagcggcg agctttttcc
     9841 gcggcagcgg ccacccacgg caactgcccc aaacccgtca ggagaatgca atgagcaaaa
     9901 agatcgctta cgtcaccggt ggcatgggtg gcatcggcac ctcaatctgc cagcgcctcg
     9961 ccaaggatgg cttcaccgtc gtcgccggct gcggccccaa ctcgccgcgc aaggacaagt
    10021 ggctcgccga gcagaaggcg ctgggctttg acttcgtcgc ctcggaagga aacgtcgccg
    10081 actgggacag cacggtcgct gccttcgcca aggtcaaggc cgaagttggc gaaatcgacg
    10141 tcctggtcaa caacgccggc attaccaagg acggtgtctt ccgcaagatg accccggccg
    10201 actggaaggc cgtcatcgat accaacctca actcgatgtt caatgtcacc aagcaggtca
    10261 tcgacggcat gatggatcgc ggctggggtc gcgtcatcaa catctcgtcg atgaacggcc
    10321 agaagggcca gttcgggcag accaactact cgacggccaa ggccggcatg cgcggcttca
    10381 ccatggcgct ggcgcaggaa gttgccggca agggcgtcac cgtcaacacc gtgtcgccgg
    10441 gctacatcgg caccgacatg gtcaaggcga tccgcgagga cgtgctgcag aagatcgtcg
    10501 gcacgatccc agtcaagcgt ctcggcgagc cggccgagat cgcctcgatc atcgcctggc
    10561 tcgcctcgga ggaatcaggc ttcgccacgg gggccgactt ctcgctcaac ggcggcaact
    10621 acatgggctg acgcgcagcg tcggcgtttc ccagccccgg ccgcgtgccg gggttttcgt
    10681 ttctgcggag caacccattg atgactgacg aagccaactt tagcggccga attcgcgtcg
    10741 accgtgaggc cgactgggcg accctgaccc tggtcaatcc gggacggcgc aatgcgatca
    10801 accgggcgat gtggtttgct atcgcggcgg cgttgaatgg gctggccgaa gacgacgcat
    10861 tgcgctgcct g
//
LOCUS       DATDAA010000432         2674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_382104,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000432 DATDAA010000000
VERSION     DATDAA010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382104"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(398..1084)
                     /locus_tag="VIS73_12940"
     CDS             complement(398..1084)
                     /locus_tag="VIS73_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764106.1"
                     /translation="MKTTILSTLAATAVLFAAAPSLADKFEAVHSINYPDARQAEAAL
                     EGLMTDPAMKGAKLTLYAREFGEGGAAHLVVQDFDSYAEYMDSTAKRLASPGWTRYLL
                     DTSASAHYVGSDLAMVVDDHGAARRSAGYLAAYLINTTDPATYRAAIADLNKAVGNPG
                     VLRLIAFRTGTMAATHAVLIGGSDFKAVNEYLDKLFGSDAFAAFTAKVGPGRKVVGIQ
                     MYRRVATWGD"
     gene            complement(1271..>2674)
                     /locus_tag="VIS73_12945"
     CDS             complement(1271..>2674)
                     /locus_tag="VIS73_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5764107.1"
                     /translation="RDQSALSRMPTDSLAAYQSYLLGKHRMGARTSAGIAAAAEYFKQ
                     AVELDPDFAQAHVGLAETMILSSEYGSLSRTELAGAAMPLLERALALDDHLSSAHTAI
                     GAVRSKSSDTAGAEAAFRRAIELDHNNAVAFHWYADVLLLNRSQPESALALIQRAVEL
                     DPLSPAVIATLGQVLEALGRFDEARSQYESILEIDAASPVGYFLIGDFERFVNGRLDE
                     AVRWYRESIARDPGNAAVQGSISISYLELGDLQQTALWMDRAMAQNPHQYMPVAAGML
                     LAARLHRESDSLARASRLREIWRMDGLSLAVSAYLGDYQTILDVGADLYPELSCSAAT
                     GVSRRNIGQAINLSLARMEHGERECAARLLEGVLSVTSRMPRMGRFGFGITDVEAQSS
                     LGRTDAALAALRQAIDAGWRLSWGLPVELRPQLAALRNEPEFAAMTAEIERDMSIQLA
                     RVRQMEAAGQLAAIPER"
BASE COUNT          446 a          892 c          859 g          477 t
ORIGIN      
        1 aggcatcgtt gaccggttcg gggccttata gagaagtcgc caggcactcc ggaagcgcct
       61 caatttgttg ggaatagttc gcgttggaac gcgctgtagt cccgtacggg aagtgtcttc
      121 ggcagtaccg attcgtcgct aacacgtctg cgtcgggtcg ttagcggaca tgtcgacgcg
      181 gctggggcgg gtctaccggc taacgtcagt taccggccgg aagcgatcgt agcgggagca
      241 aacagaaacc ccggcgcgtg gccggggctc cgcagtcggt tcaggccttt cccggtcagg
      301 cgtgcccctg ccagaaaggc aagaccggcg acgccttgcc agcccggccc ggcccggcct
      361 tacggcccga ccggatcggc cgacagaagc tacggcgtca gtctccccag gtcgccacgc
      421 gtcggtacat ctggatgccg accaccttgc gacccgggcc cacctttgcg gtgaacgcgg
      481 cgaaggcgtc actcccaaag agcttgtcga ggtactcgtt gacagccttg aagtctgatc
      541 cgccgatcaa cactgcgtgt gtcgcagcca tcgtgccagt gcggaacgcg atcagccgca
      601 ggacacccgg gttgccgacc gccttgttga ggtcggcgat cgccgcccgg taagtcgcgg
      661 ggtcagtggt attgatgagg taggccgcga ggtagccagc gctgcgccgg gccgcgccgt
      721 ggtcgtcgac gaccatcgcc aggtcactgc cgacgtagtg cgcggatgcg ctcgtgtcga
      781 gcaggtagcg cgtccagccc gggctggcga ggcgcttcgc cgtcgagtcc atgtactcgg
      841 catagctgtc gaagtcctga acgacgaggt gcgcggcgcc cccctcgccg aactcccgcg
      901 cgtagagcgt gagcttggcg cccttcatgg ccgggtccgt catcagcccc tcgagggcgg
      961 cctccgcctg acgggcatcg ggatagttga tggagtgcac cgcctcgaac ttgtcggcga
     1021 gcgacggtgc cgccgcgaac agcaccgcgg ttgctgccag agtcgacaga attgttgtct
     1081 tcatcgtttt gcttccccct gattgcgctg gtgcgcgtat tggacgtggc gtcgtgccag
     1141 caagtcctcc cccctctggg aagacccgtg ccgcagtatg gacacaatcg ccacctcggc
     1201 aacgcctgcc gcgggccgcc ggcatcgcgt caggatgcct gtcacggcat cccgcctggg
     1261 gaacgcgagg tcatcgctcc gggatggcgg ccagttgccc cgccgcttcc atctgccgga
     1321 cgcgcgccag ctggatcgac atgtctcgtt ctatttcagc cgtcattgct gcgaactcgg
     1381 gttcattgcg cagggcagcg agctgtggac gcaattcgac cggcagcccc cagctaagcc
     1441 gccacccggc gtcgatcgcc tgccgcagcg ccgccagggc ggcatcggtc cgcccgaggc
     1501 tggactgggc ctcaacgtcg gtaatgccga agccaaagcg ccccatcctc ggcatgcgcg
     1561 atgtaacgct gagcactccc tcgagcaggc gtgcagcgca ctctcgttcg ccatgctcca
     1621 ttcgcgccag cgacaggttg atagcttgac cgatgttccg gcggctgact cccgtcgcag
     1681 ccgagcacga cagttccggg tatagatcgg cgccgacatc caggatcgtc tggtaatcgc
     1741 ccaggtacgc gcttaccgcc agcgacaagc cgtccatgcg ccagatttca cggagtcgac
     1801 tggctcgcgc caacgagtcg gattcgcgat gaagcctcgc tgcgagcaac attcccgcag
     1861 cgacaggcat gtactggtgc gggttttgcg ccatggcgcg gtccatccaa agtgcggtct
     1921 gctgcaagtc acccagttcc aggtagctga tcgaaatgct gccctgcact gccgcattgc
     1981 ctggatcgcg ggcgatactc tcgcgatacc agcgcacggc ctcgtcgagc cgtccattga
     2041 cgaagcgttc gaaatcgccg atcaggaagt aacccaccgg ggacgccgcg tcgatctcga
     2101 gaatgctctc gtactgggat cgtgcttcgt cgaagcgccc cagcgcctcc agcacctggc
     2161 cgagcgtggc aatcacggct ggcgacagcg gatccagctc cacggcgcgc tgaatcaacg
     2221 ccagcgcgct ctcgggctgg ctccggttca gcaacagaac gtcggcatac cagtggaatg
     2281 ccaccgcgtt gttgtgatcg agttcgatcg cgcgccgaaa ggctgcctcc gcacctgccg
     2341 tgtcgctgct cttgctgcgg acggcgccga tcgcggtatg cgcagacgaa aggtggtcgt
     2401 cgagagccag cgcccgttcc agaagaggca tggcggcgcc tgccaactct gttcgcgaca
     2461 ggctgccata ctcggagctc aggatcatcg tctcggcgag gcccacgtgc gcctgagcga
     2521 agtcggggtc cagctcgacg gcctgcttga agtattcggc cgcagctgcg atcccggcac
     2581 tcgtcctggc acccatccgg tgtttcccga gcaggtacga ctggtacgcc gccaggctgt
     2641 cggtcggcat cctcgacagc gcagattggt cccg
//
LOCUS       DATDAA010000433        14558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_206931,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000433 DATDAA010000000
VERSION     DATDAA010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 14558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14558
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206931"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..670)
                     /locus_tag="VIS73_12950"
     CDS             complement(164..670)
                     /locus_tag="VIS73_12950"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEY5764108.1"
                     /translation="MQKILRPIALSLVLLAPLVVQSQQAFDPGVAKPKVPGDYYVGAE
                     KAVYHVTQDADEKGYLAILGNLRNHHNALVATGVTPELKVVINGSGIRMLTLAQELEF
                     EANARLPGAIKEAKARGVQFEVCYNTLTGRKIKFSDLYDAKSEDIIPAGVAEVARLQR
                     MGYAMIKP"
     gene            complement(691..1023)
                     /locus_tag="VIS73_12955"
     CDS             complement(691..1023)
                     /locus_tag="VIS73_12955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HEY5764109.1"
                     /translation="MEIEVNGRQVVTDNEGYLANLEDWSEDLANAIAARESLEMTPDH
                     WEIVNFLRRGYEETGVVPNIRQLQKLLANEYGAEKADKTYLYELFPYGPAKQACRIAG
                     LPKPTGCV"
     gene            1208..1540
                     /locus_tag="VIS73_12960"
     CDS             1208..1540
                     /locus_tag="VIS73_12960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HEY5764110.1"
                     /translation="MPYTINGVECETDEENFLLEADFSDEAVQVIAAAEGITLGDQHW
                     MIVNYLRDKYRDDGHTPNFRNMLKELEELHPGHDWKKDLYDLFPEQPARQAARVAGLT
                     KPFGKGGY"
     gene            1642..2502
                     /locus_tag="VIS73_12965"
     CDS             1642..2502
                     /locus_tag="VIS73_12965"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY5764111.1"
                     /translation="MHTTLVSTETLAAHLVDPAWVVVDCRFTLTDPEAGRKAWEAWRI
                     PGARYAHLDQDLSSPRRRDSGRHPLPDPQRFIETLRRWGIGNSTQVVAYDDSFGAMAV
                     RLWWLLRWVGHPAVAVLDGGLPVWRRQRLPIDEATPAPRAEREPVALTPQPNAAPAVD
                     ANFVARIAADPRWRLIDARAEDRFAGEREPLDPVAGHIPGSVNRNFEDNLALDGTWLT
                     AEALRAEFTALLGDVAPDQVVHSCGSGVTACHNLLAMEHAGLAGSRLYAGSWSEWITD
                     PQRPVARGPA"
     gene            2511..3104
                     /locus_tag="VIS73_12970"
     CDS             2511..3104
                     /locus_tag="VIS73_12970"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEY5764112.1"
                     /translation="MRTTGFAAALIASLLLAPATHAADSADWVNDPDLDGIVDLLDPD
                     YQKLPINVARLPLKYAITTRYGSGERVVYTFEDPQCGFCRQLHRHLAAIGNLTVHTFV
                     LTFLGPESASRADLAWCSPDRTRAWDAIMRGGSVAATTAANCKAPTAQTMKLAGMLGI
                     NLTPTVFYADGSRMNGVKPQAEVEARLRQASAAAIGR"
     gene            complement(3209..5275)
                     /locus_tag="VIS73_12975"
     CDS             complement(3209..5275)
                     /locus_tag="VIS73_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEY5764113.1"
                     /translation="MSSTQSQFTQALRRYYTFYTGGFIGFVIVLGIFESLGVSSRVLG
                     YLFLFSTIMLYAGIGIMSKTADVAEYYVAGRRVPALFNGMATGADWMSAASFIGMAGT
                     LYATGFDGLAFIMGWTGGYCLVALFLAPYLRKFGQFTIPDFLGARFGGNVPRFIGVVA
                     AILCSFTYVVAQIYGVGIITSRFTGLEFGVGVFVGLAGILVCSFLGGMRAVTWTQVAQ
                     YIILIIAYMIPVVWLSMKHTGNPIPQIAYGYVLQQVGEREQQLNDRHSPDGAREESVR
                     QLFRERIVETEQRLQDLAAGGSIYLAQERSRRVANVTALYRANADLSAIEAAEKELTS
                     LPPDVATAQEQWTKEKANATVKASPLKSHAEAFAGIDENARDIARRNFLALVFCLMVG
                     TAALPHILMRYYTTPSVREARLSVFYSLLFIFLLYFTAPALAVLVKYEVYSNLVGSQF
                     ANLPSWVNAWKAVDAALMSITDINRDGIVQLAEITIGGDLIVLATPEIAGLPYVISGL
                     VAAGGLAAALSTADGLLLTIANALSHDVYYKMVDPNAPTARRLIVSKTLLLVVALLAA
                     YVTSMKPGNILFLVGAAFSIAGSAFFPALVCGVFWKRANQAGAVLGMLAGLGVCLYYM
                     VITYEFFGYSEPLWWDIKPIAAGLFGIPAGFLGVLVGSLLTPAPSREVQELVDHVRYP
                     NLGSDG"
     gene            complement(5275..5541)
                     /locus_tag="VIS73_12980"
     CDS             complement(5275..5541)
                     /locus_tag="VIS73_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HEY5764114.1"
                     /translation="MQLTQKHRDYWSRNLRVTGILLAIWFVVTFVAAFFARELNEVTV
                     LGFPLGFYMGAQGSLIVYVAIIFFYARYMNALDRKYGVHEGDEE"
     gene            complement(5729..7756)
                     /locus_tag="VIS73_12985"
     CDS             complement(5729..7756)
                     /locus_tag="VIS73_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEY5764115.1"
                     /translation="MIFFGGRLKRLFYIYAGSFVMLALALAWAERHGLSQRAIGFAFL
                     SYTVILYALIGLTARTHDVAEYYVAGRKVPALLNGMATAADWMSAASFIGLAGTLYLT
                     GYQGLAFVMGWTGGYVLVALLLAPYLRRFGQFTIPDFLGARYGGILPRLGGVVAAVLA
                     SFVYLVAQIYGVGLVASRFVGLQFEIGVMVGLAGILVCSFLGGMRAVTWTQATQYLIL
                     VVAYLTPVAILSYQVTGNALAPVAYGQVLQKIGAVEPAIFGDPREIAVRAEYGKRAEA
                     LQARIAGLPDSLREEREAFSAEINRLKLADAPMREIIEVERHRRDLPSTTDEARSRWE
                     QSWREANARQAPPAPHAEAFPAPNDEARTRSQINFLALVFCLMVGTAALPHILMRYYT
                     TTSVGDARMSVFWSLLFILALYLVAPAYAVLAKYEIYEKLVGIAISDLPNWIHAWGKL
                     GLVAIEDVNGDGRLQLAELSLNPDVIVLAMPEIAGLPYVISGLVAAGALAAALSTADG
                     LLLTIAGSLSHDIYYRILRPQAAPHWRLVVSKSVLMVVAVLAAAVATQRPATILSLVS
                     WAFSIAAASFFPAMVLGIFWRRANHAGAVVGMTAGLWTTIYYLVRTEFDSLPWLGVYG
                     IGMEPWLGIQSTAAGAWGVLVGFLVMIVVSLLTRPPSARTREFVDELRRPM"
     gene            complement(7821..8528)
                     /locus_tag="VIS73_12990"
     CDS             complement(7821..8528)
                     /locus_tag="VIS73_12990"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY5764116.1"
                     /translation="MNLMEMLGLGDSGSIKLSEAQMIRLLAWRDRPPVDMELVHPQQR
                     FTVVDVEASGLDLTDDYLLAIGAVSVDRGIIDSAQVFEASLATASDRLPTALPADAIV
                     RTRPAQDPAEALIGFLEFAGRSVLVSHRADFPRTMIERAVQRHLGIEFEPVWLDLAYI
                     LPGLFDSTTDFSTSLDQWLSRFSITNPAPHNALADAYVVARLLQRTIARAVADGLSTP
                     EALRDFEQRRRRAPRSY"
     gene            complement(8538..10451)
                     /locus_tag="VIS73_12995"
     CDS             complement(8538..10451)
                     /locus_tag="VIS73_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEY5764117.1"
                     /translation="MSGSAVTNMVAKDTEQFLVRHEPFDAMEAESLAYLVSKAKLAYY
                     PKGSVLLDPEHGAPPHLFIVRDGKVQRREVSELPTLLQLTGTLGAGDCFPLGAVMGQR
                     PSTNRYVAIDDVSVLLLPADDYLRLLQMSQVFGLFCTRYIAGLFNQSRQQLRLQYAQR
                     ASEQQTMSSPIETLLKRDAASVQAETPISAVLERMVNEHIGSMVIADPEGKPLGIFTQ
                     SDVAKRIVLPKLSLDQPIGSVMSPSPHVMTVGGTIYDAAVAMAMHGVRHVVVVNAVGR
                     LRGIVSERDLFAIQRVGLRQIRQSIESAPDVDTLKQTSGDVRQLAVNMLAQGVGSEQL
                     TQFISSLNDALTRRVIELNLARHDLSGIEWAWLSFGSEGRDEQTFSTDQDNGIIFVCP
                     ELMDEEKLTARFLEFAKEVNRDLDACGFPYCTGNIMASNPEWCLSLEGWQDKFSKWIR
                     SPDPTALLNATIFFDFRALYGETHLADTLRSWLFTLSQPNTIFLRMLAANALTADAPL
                     GRFRDFVTDGEKEDAGTIDLKKSGARLFVDGARVYSLATGTPNTNTQQRLRAACTKVG
                     MPKDEIAALIESFNFIQQLRLRNQHMQAQSGREPNNRVRPDDLNELDRHILKESFKQA
                     RKLQSRLRIDYHV"
     gene            complement(10712..12694)
                     /gene="acs"
                     /locus_tag="VIS73_13000"
     CDS             complement(10712..12694)
                     /gene="acs"
                     /locus_tag="VIS73_13000"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY5764118.1"
                     /translation="MSQNESGSHEVRIYNPPADLAAKAAVSGMDAYKALCAEAERDYE
                     GYWGRLAREHLVWKQPFTQVLDESKRPFFKWFADGKLNVSYNCLDKNVEAGLGDKTAI
                     IFEADNGEVSKVSYKELLVKVCKMANALKAQGVKKGDRVVIYLPMTIEGIVAMQACAR
                     IGAIHSVVFGGFSAQSVRDRISDAGAVMVITSDGQFRGGKSLPLKNIVDEAVALGGCD
                     SLKSVIVFKRTGGECKMVAGRDQWMHELVAKQADTCEPEWVEAEHPLFLLYTSGSTGK
                     PKGVQHSSGGYLLHAALTMKWTFDIKPDDIFWCTADIGWVTGHTYIAYGPLACGATEV
                     VFEGVPTYPDAGRFWKMIQDHKVSIFYTAPTAIRSLIKAADANAAIHPKSYNLKSLRI
                     LGSVGEPINPAAWEWYHEHVGGGRCPIVDTFWQTETGGHMITPLPGATPLVPGSCTLP
                     FPGIQFAVVDETGTELEWGKGGILVCKKPWPSMIRTIWGDPERFKTSYYPEDFQGKYY
                     LAGDGAIRDAKTGYFTITGRIDDVLNVSGHRMGTMEIESALVAHELVAEAAVVGRPDD
                     VTGEAICAFVVLKRPRPTGDEAKAIIKDLQTWVGKEIGPIAKPKDIRFGDNLPKTRSG
                     KIMRRLLRSLAKGEEVTQDISTLENPAILEQLKPGA"
     gene            13044..13574
                     /locus_tag="VIS73_13005"
     CDS             13044..13574
                     /locus_tag="VIS73_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HEY5764119.1"
                     /translation="MQSTIPVLERLIFWSRWLQAPLYLGLIVAQIVYVVQFMVELSHL
                     VHKVFEGHLAETDTMLIVLGLIDVVMIANLLIMVIIGGYETFVSRLELDGHPDKPEWL
                     SHVNAGVLKIKLSTAIIGISSIHLLKSFINAENLTQQTLQWQVVIHMVFIISALAMAW
                     TDRVMNQTLQLSKKAH"
     gene            13599..>14558
                     /locus_tag="VIS73_13010"
     CDS             13599..>14558
                     /locus_tag="VIS73_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020752349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEY5764120.1"
                     /translation="MTTIRQEDLIASIADAFQYISYYHPLDYITALGKAYEREESPAA
                     KDAIAQILTNSRMSAEGHRPICQDTGIGMVFLKVGMNVRWEGATMSLQKMVDEGVRRA
                     YANADNPLRASVLADPAGARKNTKDNTPAVVHFEIVEGDHVEVICAAKGGGSEAKAKF
                     AMLNPSDDLVEWILKTVPTMGAGWCPPGILGVGIGGTPEKAMLLAKEAIMAPVDIHEL
                     KERGPKTRAEELRLELFEKVNALGIGAQGLGGLTTVLDVKVLDYPCHAANLPVALVPN
                     CAATRHCHFHLDGSGPAKLEPPQLSDWPDVTWTADLKSATRVDL"
BASE COUNT         2736 a         4737 c         4651 g         2434 t
ORIGIN      
        1 tccgatcgcg tcgcaaaaca caatggtcag cacggcgccg cgtcatggtt gcggaagact
       61 gtctgcatgc tttctggttc ccctgccccg ctcaagcgcc tgttctgatt cgccaaacgc
      121 ctggggaccg gcgtttggcg agtgacggct gcggtgctgc gccttagggc ttgatcatcg
      181 cgtagcccat gcgctgcagg cgggcgactt cggcgacacc ggctgggatg atgtcctcgc
      241 tcttagcgtc gtaaaggtcg gaaaacttga tcttgcggcc ggtcaatgtg ttgtagcaga
      301 cctcgaactg gacgccacgc gccttcgctt ccttgatggc gccgggcaga cgggcgttgg
      361 cctcgaattc gagctcctgg gccagcgtca gcatgcgaat gccgctgcca ttgatgacga
      421 ccttcagctc tggagtaaca ccggttgcca ccagcgcatt gtggtggttt cgcagattgc
      481 cgagaatcgc cagatagccc ttctcgtctg cgtcttgggt gacgtgatac acggcctttt
      541 cggcaccgac gtagtagtcg ccgggaacct tgggcttggc aacgccgggg tcgaatgcct
      601 gctgcgattg gacgaccagc ggggccagca acaccagcga cagcgcaatt ggtcttagga
      661 ttttttgcat tgcatctcct ccatcagttg ttacacacag ccggttggtt tgggcaaccc
      721 ggcaatccga caggcctgct tcgccggccc atagggaaag agttcgtaca gataggtctt
      781 gtctgccttt tcggcaccgt actcgttggc caacagcttc tgcagctgac ggatattagg
      841 gacgacgccg gtctcctcgt aaccgcgccg caggaaattg acgatctccc agtggtccgg
      901 ggtcatctcc agcgactcgc gcgcggcgat cgcgttggcc aaatcctcgc tccagtcctc
      961 caggttggct agatacccct cgttgtcagt aaccacttgc cgaccattca cttcgatttc
     1021 catatcccgc tcccgctttg ttcggttgat agggtgcgcg gacgtgggct cccgcacgat
     1081 ggccaatcga cacgctcgcg gtgattaggt tcggcgcata ccccaccggg gttagtcggg
     1141 acgggcgatg gaggccgcct cccggctcgg ctagcatcaa cgttcccgct atccggagga
     1201 tggacgcatg ccctacacga tcaacggcgt cgagtgcgaa accgacgagg agaacttcct
     1261 gcttgaggcc gacttcagcg acgaagcggt acaggtcatc gccgctgccg aaggcatcac
     1321 gctcggcgat cagcactgga tgatcgtgaa ctacctgcgc gacaagtacc gcgacgacgg
     1381 ccacaccccg aatttccgca acatgctcaa ggagctcgag gagcttcacc ccggccacga
     1441 ctggaaaaag gatctgtatg atcttttccc cgagcagccg gcccggcagg ccgctcgcgt
     1501 cgccggccta accaagcctt tcggcaaggg cgggtactga gcaccggctt ccccgcggag
     1561 gtcaggcaac ccgaaaaggg cccggagtat ccttgaccga agcagggcga cggctctgcc
     1621 aacaccacgg cggagccccg catgcacacc acgctcgtca gcaccgaaac actcgcggca
     1681 catctggtcg accccgcgtg ggtcgtggtc gattgccgct tcacgctgac cgaccccgag
     1741 gcggggcgca aggcctggga agcctggcgc attcccggcg cgcgctacgc gcacctcgac
     1801 caagacctct cgagcccgcg ccgccgcgac agcggccgcc atccgctgcc cgacccacaa
     1861 cgcttcattg aaacactgcg ccgctggggc atcggcaact cgacgcaggt cgtggcctac
     1921 gacgacagct tcggcgccat ggccgtccgc ctctggtggc tgctgcgctg ggtcggccat
     1981 ccggccgtcg ccgtgctcga cggcgggctg ccggtctggc gccgccagcg cctgccgatc
     2041 gacgaagcta ccccggcccc gcgcgccgag cgcgaaccgg tagcattgac accccaaccc
     2101 aacgcggcac cggccgtcga tgcgaacttc gtcgcgcgca tcgccgccga tccacgctgg
     2161 cggctcatcg acgcccgtgc cgaagaccgc ttcgccggtg agcgcgaacc gctcgacccg
     2221 gtcgccggcc acatcccggg cagcgtcaat cgcaacttcg aagacaacct cgcgctcgac
     2281 ggcacctggc tcaccgccga ggccctgcgc gccgaattta ccgccctgct gggcgacgta
     2341 gcgcccgacc aagtcgtcca cagctgcggc tcgggcgtca cggcctgcca caacctgctg
     2401 gcgatggaac acgccggcct tgccggcagc cggctctacg cgggctcctg gagcgagtgg
     2461 atcaccgacc cgcagcgccc ggtcgcgcgc ggcccggcct gagacgaatc gtgcgcacaa
     2521 ccggctttgc ggcagcgctg atcgcctccc tcctgctggc cccggcgacg cacgccgccg
     2581 attccgccga ctgggtcaac gaccccgacc tcgacggcat cgtcgacctg ctcgacccgg
     2641 actaccagaa actgccgata aacgtcgcgc ggctgccgct caagtacgcc atcaccaccc
     2701 gctacggcag cggcgagcgc gtcgtctaca ccttcgaaga cccacagtgc ggcttctgcc
     2761 ggcaactgca ccgccacctc gcggcgatcg gcaacctcac cgtgcacacc ttcgtcctca
     2821 ccttcctcgg ccccgaatcg gcaagcaggg ccgacctcgc ctggtgcagt cccgaccgca
     2881 cgcgggcgtg ggacgcaatc atgcgcggcg gcagcgtcgc cgcgaccacg gctgccaact
     2941 gcaaggcacc gaccgcgcag acgatgaagc tcgccggcat gctcggcatc aacctcaccc
     3001 ccaccgtctt ctacgccgac ggcagccgca tgaacggcgt caagccgcag gcggaagtcg
     3061 aagcgcgtct gcgccaggcg agtgccgcgg ccatcggacg ctagggcaaa gttggtcgcc
     3121 gagactgaac accgtacagc gcgatatctg aacgccgtct ggcgctatca aagacagatt
     3181 ttctgcaccc catcttggct ggccaacatt agccgtcgct gcccagattc gggtagcgaa
     3241 cgtggtcaac gagttcctgt acctcgcggc tcggcgccgg cgtcagcagg ctgccgacaa
     3301 ggacgccgag gaagccggcc gggatgccga agaggccggc agcgatcggc ttgatgtccc
     3361 accagagcgg ctcgctgtag ccgaaaaact cgtaggtgat gaccatgtag tagaggcaga
     3421 cgccgaggcc ggcgagcatg ccgagcacgg cgccggcctg gttggcgcgc ttccagaaga
     3481 cgccgcagac cagcgccggg aagaacgccg agccggcgat cgagaaggcg gcgccgacca
     3541 ggaagaggat gttgcccggc ttcatcgagg tgacgtaggc ggccagcaac gcgacgacca
     3601 gcaacagcgt cttggagacg atcagccgcc gcgcggtcgg cgcgttgggg tcgaccatct
     3661 tgtaatagac gtcgtgcgag agcgcgttgg cgatcgtcag cagcaggccg tcggccgttg
     3721 acagggcggc tgcgaggccg ccggcggcga ccagcccgga gatgacgtag ggcaatccgg
     3781 cgatctcggg cgtcgccagc acgatcaggt cgccgccgat ggtgatttcg gcgagttgga
     3841 cgatgccgtc gcggttgatg tcggtaatcg acatcagcgc cgcgtcgacg gccttccagg
     3901 cgttcaccca actcggcaga ttggcgaact gcgagccgac gaggttgctg tagacctcgt
     3961 acttgaccag cacggccagc gccggtgcgg tgaagtagag caggaagatg aacagcagcg
     4021 aatagaacac cgacagccgc gcttcgcgta ccgatggcgt cgtgtagtag cgcatcagaa
     4081 tgtgcggcag cgcggcggtg ccaaccatca gacagaaaac cagcgcgagg aagttgcggc
     4141 gcgcgatgtc acgggcattc tcgtcaatgc cggcaaaggc ctcggcgtgt gacttgagcg
     4201 gcgaggcctt gaccgtggcg ttggccttct ccttggtcca ctgctcctgc gcagtcgcga
     4261 cgtcgggcgg cagactcgtt agctcctttt ctgcggcctc gatcgcgctc aggtcggcgt
     4321 tggcacggta cagcgcggtg acgttggcga cccgccggct gcgctcctgc gcgagataaa
     4381 tggagccgcc tgcagcgagg tcctgcaagc gctgctcggt ctcgacgatg cgctcgcgga
     4441 agagctggcg gacgctttcc tcgcgcgcgc cgtcgggcga atggcggtcg ttgagctgct
     4501 gttcgcgttc gccgacctgc tgcagtacgt agccgtaggc gatctgtggg atcgggttgc
     4561 cggtgtgctt catcgacagc cagaccaccg gaatcatgta ggcgatgatg aggatgatgt
     4621 actgcgcgac ctgcgtccag gtcaccgcgc gcatgccgcc gaggaaggag cagaccagga
     4681 tgccggcgag gccgacgaag acgccgacgc cgaactcgag cccggtaaag cggctggtga
     4741 taatgccaac gccatagatc tgcgcgacga cgtacgtgaa cgaacagagg atcgcggcga
     4801 ccacgccgat gaaacgcggc acgttgccgc cgaagcgggc gccgagaaaa tcagggatcg
     4861 tgaactggcc gaacttgcgc agatagggcg cgagaaacag cgccaccagg cagtagccgc
     4921 cggtccaacc catgatgaag gcgaggccgt cgaaaccggt cgcgtagagc gtgccggcca
     4981 tgccgatgaa ggacgcggcg ctcatccaat cggcgccggt ggccatgccg ttgaacagcg
     5041 ccggcacgcg ccggccggca acataatatt cggcgacatc ggcggtcttc gacataatgc
     5101 cgatgccggc gtagagcatg atcgtcgaaa acaggaacag gtagcccagc acccggctcg
     5161 atacgccgag cgactcgaag atgccgagga cgatgacaaa accgatgaaa ccgccggtat
     5221 agaaggtgta gtaccgacgc agcgcctgcg tgaactggct ctgggtcgac gacatcactc
     5281 ctcatcccct tcgtggacgc cgtacttgcg gtccagcgcg ttcatgtagc gcgcgtagaa
     5341 gaagatgatt gctacgtaga cgatcagcga tccctgggcg cccatgtaga agccgagcgg
     5401 aaagccgagc acagtgactt cattgagttc acgcgcgaag aacgcggcga cgaaggtgac
     5461 gacgaaccag atggcgagca ggattccggt tacccgcagg ttgcgcgacc aatagtcgcg
     5521 atgcttctgc gtaagctgca tgatatgtcc tcctcctttt gcgccgcgcc tccgggccag
     5581 ttaatccggc ctcgatgtgt tctggcgcgc cggcgctagg cgccacccgt acgctcgccg
     5641 ggccgcaggg gatcgtccat gctattggtc cgacgctgac aggaggctta cggcccgccc
     5701 ggcaggctac cgcggcacga ccctcagctc acatagggcg ccgcagttca tcgacgaatt
     5761 cacgcgtcct tgccgatggc ggtctggtca gcagactgac gacaatcatc accagaaagc
     5821 cgaccagcac gccccaggcg ccggcggccg tcgactggat gccgagccag ggttccatgc
     5881 cgatcccgta gacgcccagc cagggcaggc tgtcgaattc ggtgcgaacc agatagtaga
     5941 tcgtcgtcca caggccggcc gtcatgccga ccacggcgcc ggcatggttg gcgcgacgcc
     6001 agaagatgcc aagcaccatc gccggaaaga aggacgcggc ggcgatcgag aaagcccacg
     6061 aaacgaggct gaggatcgtc gccggtcgtt gcgtggcgac cgcggcggcg agcaccgcga
     6121 ccaccatcag caccgatttc gaaaccacca gccgccagtg cggcgccgcc tgcgggcgga
     6181 ggatgcgata gtagatgtcg tgcgacagcg atccggcaat cgtcagcagc aggccgtcgg
     6241 cggtcgacag cgctgcggcc agcgcgccgg cggcgaccaa gcccgagatg acgtagggca
     6301 agccggcgat ctcgggcatc gccagcacga tcacgtcggg gttgagcgac agttcagcca
     6361 attgcaggcg accgtcaccg ttgacgtcct cgatcgccac cagcccgagc ttgccccagg
     6421 cgtgaatcca gttcggcagg tcgctgatgg caatgccgac cagcttctcg tagatctcgt
     6481 acttggcaag caccgcgtag gccggcgcga ccaggtacag cgcgaggatg aacagcagcg
     6541 accagaatac cgacatccgc gcgtcgccaa ccgaggttgt cgtgtagtag cgcatcagga
     6601 tgtgcggcag cgccgcggtg ccaaccatca ggcagaacac cagcgcgagg aaattgatct
     6661 ggctgcgcgt gcgcgcctcg tcgttgggcg ccggaaaggc ctcggcgtgc ggtgccggtg
     6721 gcgcctggcg agcgttcgcc tcgcgccagg actgttccca gcgcgagcgg gcctcgtcgg
     6781 tcgtgctcgg caggtcgcgg cggtggcgtt cgacctcgat aatctcgcgc atcggcgcgt
     6841 cggccagctt gagtcgattg atctcggcgg aaaacgcctc gcgttcctcg cgcagtgaat
     6901 cgggcaggcc ggcgatgcgc gcctgcagtg cttcggcgcg ctttccgtac tcggcacgga
     6961 cggcgatctc gcgcggatcg ccgaagatcg ccggctcgac ggcaccgatc ttctgcagca
     7021 cctggccgta ggcgaccggc gccagcgcgt tgccggtcac ctggtaagag aggatcgcca
     7081 ccggcgtcag gtaggcgacg accaggatca ggtattgcgt tgcctgcgtc caagtcacgg
     7141 ctcgcatacc gccgaggaag gagcagacga ggatgccggc gaggccgacc atgacgccga
     7201 tctcgaactg caggccgacg aagcggctgg cgaccaggcc gaccccgtag atctgcgcaa
     7261 ccagatagac aaaggaggca agcaccgcgg cgaccacgcc gcccagccgg ggcaggatgc
     7321 cgccgtagcg ggcgccaagg aaatcgggaa tcgtgaactg gccgaatcgc cgcaggtaag
     7381 gcgcgagcaa cagggcgacg agcacgtaac caccggtcca gcccataacg aaggcgagcc
     7441 cctggtaacc cgtgaggtag agcgtaccgg ctagcccgat gaacgacgcg gcgctcatcc
     7501 agtcggccgc ggtcgccatg ccgttgagca gcgccggcac cttgcgccct gccacgtagt
     7561 actcggcaac gtcgtgcgtg cgcgccgtca gcccgatcag cgcgtagagg atcaccgtgt
     7621 aggagaggaa ggcgaagccg atcgcgcgtt gcgagagtcc atggcgctcg gcccaggcga
     7681 gcgccagcgc gagcatcacg aaggatccgg cgtagatgta gaacagccgc ttgaggcggc
     7741 cgccgaagaa gatcatcgcc gcggaggcag ggatggcgcc gcgcccgccc ccacccggtc
     7801 gcgctgcggc gcagccccca tcagtagctg cgcggcgcgc gccggcgacg ctgctcgaag
     7861 tcgcgcagcg cctcgggcgt cgacagaccg tcggcaacgg cccgcgcgat ggtccgctgc
     7921 aacaggcggg cgactacgta ggcgtctgcc agcgcgttgt gcggtgccgg gttggtgatc
     7981 gaaaagcgcg atagccactg gtcgagcgac gtcgaaaaat cggtggtgct gtcgaacagc
     8041 cccggcagga tgtaagcgag gtcaagccag accggctcga attcgatgcc gaggtggcgt
     8101 tgcaccgctc gctcgatcat tgttcgcggg aaatcggcgc gatgcgaaac caggaccgag
     8161 cgacccgcga attcaaggaa tccgatcagt gcctcggccg ggtcctgcgc cgggcgggtt
     8221 cgcacaatgg cgtcggccgg cagcgccgtg ggcaatcgat cagacgcggt ggccagcgag
     8281 gcctcaaaga cctgcgcgct gtcgatgatg ccgcgatcga ccgacaccgc gccgatcgcc
     8341 agcaggtagt cgtcggtgag atcgaggccg ctggcctcga cgtcgaccac cgtgaagcgc
     8401 tgctgcggat gaacgagttc catgtccacc ggcggccggt cacgccaagc cagcaaccgg
     8461 atcatctgtg cttcgctcag cttgatcgag cccgaatcac cgagtccgag catttccatc
     8521 agattcatcg cgctgcctca gacgtggtag tcgatgcgca agcgggactg cagctttcgc
     8581 gcctgcttga acgactcctt gaggatgtgc cggtcaagct cgttcaggtc gtcggggcgc
     8641 acccggttgt tcggctcgcg acccgactgc gcctgcatgt gctggttgcg caagcgcaac
     8701 tgctggatga agttgaatga ttcgatcagc gccgcgatct cgtccttggg cattcccacc
     8761 tttgtgcagg ccgcgcgcag ccgctgctgg gtattggtat tgggagtacc ggtcgccaac
     8821 gaatagacgc gcgcaccgtc gacgaacagg cgtgcacccg atttcttgag gtctatcgtg
     8881 cccgcatcct ccttctcacc gtcggtgacg aagtcgcgga aacgaccgag aggcgcgtcg
     8941 gcggtcaggg cattggcggc gagcatgcgc aagaagatcg tgttcggctg cgaaagcgtg
     9001 aacagccagc tgcgcaaggt gtcggccaga tgggtctctc cgtagagcgc ccgaaaatcg
     9061 aagaagatcg tcgcattgag cagcgcggtt ggatcgggcg aacgaatcca cttgctgaac
     9121 ttgtcctgcc aaccctcgag cgaaagacac cactcggggt tgctggccat gatgttaccg
     9181 gtgcaatagg gaaagccgca ggcgtcaagg tcgcggttga cctccttggc gaattcgaga
     9241 aagcgggccg taagcttttc ctcgtccatc agctcggggc agacgaagat gattccgttg
     9301 tcctgatcgg tgctgaaggt ctgttcgtca cgcccctcgg aaccgaagga caaccacgcc
     9361 cactcgatac ccgacaggtc gtgccgggcc aagttgagct cgatcacgcg ccgcgtcagc
     9421 gcgtcgttga gcgaggagat gaactgggtc agctgctcgg aaccgacacc ctgcgccagc
     9481 atgttgacgg cgagctggcg gacgtcgccg ctggtctgct tgagcgtatc gacgtcgggc
     9541 gccgactcta tcgactggcg aatctggcgc aggccgacac gctggatggc gaacaggtcg
     9601 cgttcggaga cgatgccgcg cagccgcccg accgcattga cgacgacgac gtggcggacg
     9661 ccgtgcatcg ccatcgccac ggccgcatcg tagatcgtcc cgccgacggt catcacgtgt
     9721 ggactcgggc tcatcaccga accgatcggc tggtcgagcg acagcttggg cagcacgatg
     9781 cgcttggcta cgtcggattg ggtgaagata ccgagcggct tgccttccgg atcggcgatc
     9841 accatcgagc cgatgtgttc gttaaccatg cgctcgagga cggcactgat cggcgtttcg
     9901 gcctggaccg acgcagcatc gcgcttgagc aaggtctcga tcggcgaact catggtctgc
     9961 tgttccgagg cgcgctgcgc gtactgcaag cgcagctgct ggcgcgactg gttgaacagc
    10021 ccggcgatgt agcgcgtgca gaagaggccg aacacctggc tcatctgcaa gaggcgcagg
    10081 taatcgtcgg ccggcagcag cagcaccgag acgtcatcga tggcgacgta acggttggtc
    10141 gacgggcgtt gtcccatcac cgcgccgagc gggaagcagt cgccggcacc gagtgtcccg
    10201 gtcagctgca gcagcgtcgg cagctcgctg acttcgcgtc gctggacctt gccgtcgcgg
    10261 acgatgaaca ggtgtggcgg cgcgccatgc tcggggtcga gcagaaccga gcccttcggg
    10321 taataggcca gcttggcctt ggaaacgagg taggcaagcg attccgcctc catcgcatcg
    10381 aatggctcgt gccggacgag gaattgctcg gtgtcctttg ccaccatgtt ggtgaccgcg
    10441 ctcccactca tttctgcctc cgtaattgag tgcccggagc ccgaaagaca gacgggccgg
    10501 cactttcgca ccggcccgaa ccgtttcttg cggcgccgca tccgggcgcc attcttatat
    10561 cacaccacct tggccacgtt attgtcgcga aacaacccgc aacgccgcgg ctcgatggcg
    10621 atccccccta ccccgccgaa gggctgccat gcagccgttt cgacaattca ttacgaccgg
    10681 cccgattggc ccagccaaaa caagcgccga atcaggcgcc cggcttgagc tgctccagaa
    10741 tcgccgggtt ctccagcgtc gagatgtcct gggtgacttc ctcgcccttg gcgagcgagc
    10801 gcagcaggcg gcgcatgatc ttgcccgagc gcgttttcgg caggttgtcg ccgaagcgga
    10861 tgtccttcgg cttggcgatc ggcccgatct ccttgccgac ccaggtctgc agatccttga
    10921 tgatcgcctt cgcctcgtca cccgtaggac gcggacgctt gagcacgacg aaggcgcaga
    10981 ttgcctcgcc ggtcacgtca tcgggacggc caacgacggc ggcctcggca accagctcgt
    11041 gggcgaccaa tgccgattcg atttccatcg tgcccatgcg gtgccccgag acgttgagca
    11101 cgtcgtcgat gcggccggta atcgtgaagt agccggtctt cgcgtcgcgg atcgcgccgt
    11161 cgccggcaag gtagtacttg ccctggaaat cttcgggata gtaggaggtc ttgaagcgct
    11221 ccgggtcgcc ccagatggtg cggatcatcg acggccaggg cttcttgcag acgaggatgc
    11281 cacccttgcc ccactctagt tcggtgccgg tttcgtcgac gacggcaaac tggatacccg
    11341 ggaaaggcag cgtgcacgaa cctggtacca gcggggttgc gcccggcagc ggggtgatca
    11401 tgtggccgcc ggtttcggtc tgccagaagg tatcgacgat cgggcaacgg ccgccaccga
    11461 cgtgctcgtg gtaccactcc caggcagccg ggttgatcgg ctcgccaacc gaaccgagga
    11521 tgcgcaggct cttcaggttg tagctcttcg gatggatcgc agcgttcgcg tcggcggcct
    11581 tgatcagcga gcggatcgcc gtcggcgccg tgtagaagat tgaaaccttg tgatcctgga
    11641 tcatcttcca gaagcggccg gcatccggat aagtcggcac gccttcgaaa accacttcgg
    11701 tcgcaccgca ggcgagcgga ccgtaggcaa tgtaggtatg gccggttacc cagccgatgt
    11761 cggccgtaca ccaaaagatg tcgtcaggct tgatgtcgaa ggtccacttc attgtcagcg
    11821 ccgcgtgaag caggtagccg ccgctcgagt gctgaacgcc cttcggcttg ccggtcgacc
    11881 ccgacgtgta gagcaggaac agcgggtgct cggcttcgac ccactcgggt tcgcaggtgt
    11941 cagcctgctt ggcaaccagt tcgtgcatcc actggtcgcg gccggcgacc atcttgcact
    12001 cgccgccggt acgcttgaag acgatgacgc tcttcagcga atcgcagcca cccaacgcaa
    12061 cggcctcgtc gacgatgttc ttcaacggca ggctcttgcc accacggaac tggccgtcgg
    12121 aggtaatcac cattaccgca ccggcgtcgc taatgcgatc acgcaccgac tgcgccgaga
    12181 agccgccaaa gacgaccgag tgaatcgcgc cgatacgggc gcaggcctgc atcgcgacga
    12241 tgccctcgat ggtcatcggc aggtagatga cgacgcggtc gcccttcttg acgccctgcg
    12301 ccttgagcgc attggccatc ttgcagactt tgaccagcag ttccttgtag ctaaccttgg
    12361 agacctcgcc gttgtcggcc tcgaaaatga tcgcggtctt gtcgcccagc cctgcttcga
    12421 cgttcttgtc gaggcagttg taggacacgt tcagtttgcc gtcggcaaac cacttgaaga
    12481 acggccgctt cgattcgtcg agcacctgcg tgaacggctg cttccagacg aggtgttcgc
    12541 gggcaagacg accccaatag ccttcgtagt cgcgctccgc ctcggcgcac agcgccttgt
    12601 aggcatccat ccccgagacg gcagccttag cggccagatc ggcgggcgga ttgtagatgc
    12661 ggacttcgtg gcttccggat tcgttttggg acatgggtca agctcctctg ttgtcttttc
    12721 gaaacatggt gctgcggaaa tttgaactga cgatggcaag gccgggcctt gggactgcga
    12781 ggccgacggc tggtgccacc cgctggcgca acgcatagcg gcgcgcaacg ataaccgaac
    12841 gattacaaca acataatctt acgtacccct tacgctgcaa tgcgccgagc aacgagcgcc
    12901 ccagcggggg caagcagaat gaccgattgg ccggcaaccc gcacgctgcg acgcaccatc
    12961 gacgatgcta gaattcgcgg ccggccgtat gcccatctgc agcgaaccgg cgcacccata
    13021 taccccaccc gcctgcgcaa gcgatgcaat cgaccatccc cgttctcgaa cgcctgatct
    13081 tctggagccg ctggctccag gctcccctct atctcggcct gatcgtcgcg cagatcgtct
    13141 atgtcgtgca gttcatggtc gaactctcgc acctcgtgca caaggtcttc gaaggtcatc
    13201 tcgccgaaac cgacacgatg ctgatcgtgc tcggcctgat tgacgtggtg atgatcgcca
    13261 acctgctgat catggtgatc atcggcggct acgagacctt cgtctcgcgc ctcgaactcg
    13321 acggccaccc tgacaaaccc gagtggctct cgcacgtcaa tgccggcgtg ctgaagatca
    13381 agctgtcgac ggcgatcatc ggcatctcgt cgatccacct gctgaagtcg ttcatcaatg
    13441 ccgagaactt gacccagcag acactgcagt ggcaggtggt gatccacatg gtcttcatca
    13501 tctcggcgct ggcgatggcc tggaccgacc gggtgatgaa ccagacgctg caactctcca
    13561 agaaagctca ctgacccgcc caccaaggaa ggaccgccat gaccacgatc cgtcaggaag
    13621 acctcattgc gtcgatcgcc gacgccttcc agtacatcag ctactaccat ccgctcgatt
    13681 acatcaccgc gctcggcaaa gcctatgagc gcgaggagtc gccggcggcg aaggacgcaa
    13741 ttgcccagat tctgaccaac agccgcatgt cggccgaggg ccatcgcccg atctgccagg
    13801 acaccggaat cggcatggtt ttcctcaagg tcggcatgaa cgtgcgctgg gaaggcgcga
    13861 cgatgagcct gcagaagatg gtcgacgaag gcgttcgccg tgcctacgcc aacgccgaca
    13921 acccgctgcg tgcctcggtg ctcgccgatc cggccggcgc ccgcaagaac accaaggaca
    13981 atacgccggc cgtcgtccac ttcgagatcg tcgaaggcga ccacgtcgag gtgatctgcg
    14041 cggccaaggg cggcggttcg gaagccaagg ccaagtttgc gatgctcaat ccctccgacg
    14101 atctggtcga gtggattctg aaaacggtac cgacgatggg cgccggctgg tgcccaccgg
    14161 gcattctcgg cgtcggcatc ggcgggacgc cggaaaaggc gatgctgctg gccaaggaag
    14221 ccatcatggc gccggtcgat atccacgaac tgaaggagcg tggcccgaag acacgcgccg
    14281 aggaactgcg gctcgagctc tttgaaaagg tcaatgcgct gggcatcggt gcgcagggcc
    14341 taggcggcct gaccacggtg ctcgacgtca aggtgctcga ctacccctgc cacgctgcca
    14401 acctgccggt ggcgctggtg cccaactgcg cggcgacccg ccactgccac ttccatctcg
    14461 acggctccgg cccggcgaaa ctcgaaccgc cgcaactgtc ggactggccg gacgtgacct
    14521 ggaccgccga cctcaaatca gcgacccgcg tcgatctg
//
LOCUS       DATDAA010000434         2544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_31736,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000434 DATDAA010000000
VERSION     DATDAA010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31736"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VIS73_13015"
     CDS             complement(<1..227)
                     /locus_tag="VIS73_13015"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764121.1"
                     /translation="MGVQPLQAAGRRAAAATLGGAIVKIIASRALVGLLWCLHWLPLP
                     VMRALGAGVGRLLYRFAGRRRRIAEINLRLC"
     gene            complement(158..1021)
                     /locus_tag="VIS73_13020"
     CDS             complement(158..1021)
                     /locus_tag="VIS73_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5764122.1"
                     /translation="MSLLFRLLSHLPLPPLHLLGTLTGWLVWALAPTYRRVLRGNLRL
                     ALGTTQAAALERAVAAESGKGLFEMPIMWCAPPERVVGLVREVSGWEVVEAARKRGAG
                     LVFLTPHLGCFEITAQYIATRIPIAVLYRIPKQPWLDRLMRQGRSRPGMTSWPADLSG
                     VRALMKSLRRGEAVGLLPDQAPKAGEGQWLPFFGRPAYTMTLAARLAEGGNQVVFVWG
                     ERLPRGAGFRVRFTLPGEPLTGDLSERAAALNREVEQLIRLRPEQYLWGYNRYKRPAG
                     APPPPPLEAQS"
     gene            1268..2431
                     /gene="metK"
                     /locus_tag="VIS73_13025"
     CDS             1268..2431
                     /gene="metK"
                     /locus_tag="VIS73_13025"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005246700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY5764123.1"
                     /translation="MTEYLFTSESVGEGHPDKVADQISDAVLDAILSEDPAGRVACET
                     LVSTGLVVMSGEITTKSHINYREIAQDTVRRIGYTHSDIGFDYKSCAILTAINRQSPD
                     IAQGVNNASDDNLDQGAGDQGLMFGYACRETDALMPFPIHFAHRIMQRQSEVRKDGRL
                     PWLRPDAKSQLTVRYVDGKPVAIDTVVVSTQHAPEISHAQLSEAVIEEVIKPVLPQEM
                     LTGNTRYLINPTGRFVVGGPHGDCGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDRS
                     AAYAGRYVAKNVVAAGLADKCEVQIAYAIGVARPVSLMVNTFGTGKIADEKIAALISQ
                     HFDLRPKGIIAELDLLRPIYAKTAAYGHFGRDEPEFTWERTNKAAALKSEAGL"
BASE COUNT          492 a          849 c          805 g          398 t
ORIGIN      
        1 aagcacaggc gcaggttgat ctcggcaatc cgccgtcgac gcccggcgaa acggtagagc
       61 agccggccga cgcccgcgcc gagcgcccgc atcacgggca gcggcaacca gtgcaggcac
      121 cagagcaggc cgaccagcgc acgactggcg atgatcttca cgattgcgcc tccaagggtg
      181 gcggcggcgg cgcgccggcc ggccgcttgt agcggttgta cccccacagg tattgctcgg
      241 ggcggaggcg gatcaactgc tcgacttcgc ggttgagcgc cgcggcacgc tcgctcagat
      301 cacctgtcag cggctcgccg ggcagcgtga agcggactcg gaatccggcg ccgcgcggca
      361 aacgctcgcc ccacacgaaa acgacctgat tccccccttc ggcgagtcgc gcggcgagag
      421 tcatcgtgta ggccggtcga ccaaagaacg gcagccattg cccctcgccg gccttgggtg
      481 cctgatcggg cagcaagccg acggcctcgc cgcggcgcag tgatttcatc agcgcccgca
      541 cgccagacag gtcggccggc catgaggtca tccccgggcg actgcgccct tgccgcatca
      601 ggcggtcgag ccagggctgt tttgggatgc ggtagaggac tgcaatcgga atccgggtcg
      661 caatgtactg tgcggtgatt tcaaaacagc cgagatgcgg ggtgaggaac acgaggcccg
      721 cgccgcgctt tcgcgccgcc tcgaccactt cccagccgct cacctcgcgc accagcccga
      781 cgacgcgctc gggcggcgcg caccacatga tcggcatttc gaataggccc ttgccggatt
      841 cggcggccac cgcacgctcc agagccgcag cttgcgtcgt gcccagcgcc aatcgaaggt
      901 tgccgcgaag gacgcggcgg taggtgggcg ccagcgccca gaccagccag cctgtcagcg
      961 taccgagcag gtgaagcggt ggcagcggca agtggctgag caggcgaaac agcaatgaca
     1021 tcgtgtcgat ggtccagggt agccaccgaa cggggggaac cgtcggggaa agtgcgaggg
     1081 cggtagtgcg aattgacccg ccgcaaggtg aaaaagtaga attgtatttt ccgccgagtt
     1141 aattggacaa cttgcggggc ggaaaccggc tgatgccggg gtaaaaatac tgctaaatcg
     1201 tcgccgctcg caccccggag ccggctacgc cggatcaacg gggtgttttc atttggagcg
     1261 gaatcacatg actgaatatc tctttacttc tgaatcggtc ggcgaggggc atcccgacaa
     1321 ggtcgccgac cagatctccg acgcggttct cgacgcgatt ctcagcgaag acccggctgg
     1381 ccgcgtggcc tgcgagacgc tggtttcaac cggtctcgtg gtgatgtcgg gcgaaatcac
     1441 caccaagtcg catataaact accgcgaaat cgcgcaggac actgttcgcc gcatcggcta
     1501 cacgcactcc gacatcggct tcgactacaa gagctgcgcg atcctcacgg cgatcaatcg
     1561 ccagtcgcct gacatcgcgc aaggggtcaa caacgccagc gacgacaatc tcgaccaggg
     1621 tgccggcgac cagggcctga tgttcggcta cgcctgccgc gaaaccgacg cgctgatgcc
     1681 cttcccgatt cacttcgcgc accggatcat gcagcgccag tcggaagtcc gcaaggacgg
     1741 acgcctgccc tggctgcgtc ccgacgcgaa aagccagctg accgttcgtt acgtcgacgg
     1801 caagcccgtg gcaattgaca ccgtggtcgt ttcgacccag cacgcccccg agatttcaca
     1861 cgcacagctg tcggaagcgg tgatcgagga ggtcatcaag cccgtactgc cgcaggagat
     1921 gctgaccggc aacacgcgct acctgatcaa cccgaccgga cgtttcgtcg ttggtggtcc
     1981 ccacggtgac tgcggcctca ccggccgcaa gatcatcgtc gatacctacg gtggtgctgc
     2041 ccaccacggc ggcggcgcat tctcgggcaa ggacccgtcg aaggtcgacc gctcggctgc
     2101 ctacgccggt cgctacgtgg cgaagaacgt cgttgccgcc gggctcgccg acaagtgcga
     2161 agtgcagatc gcctacgcga tcggtgtcgc acggccggtt tcgctgatgg tcaacacctt
     2221 cggcaccggc aagatcgccg acgaaaagat cgcggcgctg atcagccagc acttcgacct
     2281 gcgaccgaaa ggcatcatcg ccgaactcga cctgctgcgc ccgatctacg ccaagaccgc
     2341 ggcctacggt cacttcgggc gcgacgagcc ggagttcacc tgggaacgca ccaacaaggc
     2401 cgccgcgctg aaatccgaag ccggtctctg acgcgatccc gcgccgccat acagcatcgg
     2461 aaacagcccg cctctggcgg gccgtttctt ttttggcggt gccgatctgg ccctcgcgcc
     2521 aatttcgtca cgaagcgtac cgac
//
LOCUS       DATDAA010000435         3750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_346785,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000435 DATDAA010000000
VERSION     DATDAA010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346785"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VIS73_13030"
     CDS             <1..324
                     /locus_tag="VIS73_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5764124.1"
                     /translation="EALRDILNVANRQITIDGIQKAVADYFKIRVADMHSKKRSRNVA
                     RPRQVAMALAKDLTQMSLPEIGEAFGNRDHTTVLHACRTITDLRKKDGGLNRDYLVLE
                     QVLKR"
     gene            536..1642
                     /gene="dnaN"
                     /locus_tag="VIS73_13035"
     CDS             536..1642
                     /gene="dnaN"
                     /locus_tag="VIS73_13035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY5764125.1"
                     /translation="MVLVKAKRDAILEPLSAVSGIIERRHTLPILSNVLIEPGDGGLG
                     FLATDIEIEITARASIGEPGEARAVTVGARKLVDILRALPESADVTLQQQDKRLLVKA
                     GKSRFSLQTLPAEDFPRLARPGGETARFGLPQKALRHLLGMVQYAMAQQDIRYYLNGL
                     LMVVEDERLKLVATDGHRLAYAAMALESALPRQEVIVPRKTVIELGKLLADSDDPVEV
                     ELSAAQAAFRFGGVELVSKLVDGKFPDYARVIPTQHKNSLRVEREVLRQSLLRAAILS
                     NEKFRGVRWVLTDSSLKIVSSNAEQEEAQEELDVQYSGEALDIGFNVNYLLDVLNNIP
                     GAEVECLFGDAASSTLFQFASEPEFRYVVMPMRI"
     gene            1687..>3750
                     /locus_tag="VIS73_13040"
     CDS             1687..>3750
                     /locus_tag="VIS73_13040"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEY5764126.1"
                     /translation="MGEPESNAALETDSYGSESIKVLKGLEAVRKRPGMYIGDTSDGT
                     GLHHMVFEVVDNAVDEALAGYCDEIVVTIHTDSSISVRDNGRGIPTDVKEDDELKRSA
                     AEIVMTELHAGGKFDSNSYKVSGGLHGVGVSVVNALSEWLRLTIWRDGRVHEIEFSNG
                     VATGPLRVTGDTEKRGTEVHFLASKEVFGNVDYHVDILSRRLRELSFLNNGVRILLVD
                     QLSTKEESFEFSGGVKGFVEYMNRAKNVLHPNVFVATGEKDGIVADVAMQWNDTFQES
                     VLCFTNNIPQRDGGTHMTGLRAAMTRVLNKYIEEHELAKRAKVETTGDDMREGLACVL
                     SVKVPEPKFSSQTKEKLVSSEVRPAVEEVVAEHLRQFLDERPNDARVITGKIVEAARA
                     REAARRAREMTRRKGVLDSMGLPGKLADCQERDPANCELYLVEGDSAGGSAKQGRDRR
                     FQAILPLKGKILNVEKARFDKLLSSAEIATMITALGCGFGKDEYNPEKLRYHRIIIMT
                     DADVDGSHIRTLLLTFFYRQMPELVERGHIYIAQPPLYKAKLGKTERYLKDEHELNEF
                     MLRQALVDASLVAAAGREPITGDALSELAKSYLVSEAVIERASRWIDRESLVALLRGV
                     QIDLSRRETAEASAEALYAALTGNGVRVRADFDGKTDRYQIRIERSWHGNLRISNIDT
                     EFVASG"
BASE COUNT          778 a         1107 c         1204 g          661 t
ORIGIN      
        1 gaggcgctgc gcgacatcct gaacgtcgcc aaccgccaga tcaccatcga cgggattcag
       61 aaggcggttg cggactactt caagatccgc gtcgccgata tgcactcgaa gaaacgaagc
      121 cgcaatgtcg ctcggccgcg acaggtagcg atggcgctcg ccaaggatct gacgcagatg
      181 agtctgccgg agatcggcga ggccttcgga aaccgggacc atacgactgt gctgcatgcc
      241 tgccgaacca tcaccgacct gcggaaaaag gacggtggtc tgaatcgcga ctacctggtg
      301 ctggagcagg tactcaagcg atgaccggcc cgaccgtaaa agctgtgcct atcgttggga
      361 tgaattgtga gtaactcgtc gacgcgcgga cggcgcgcaa cttgttccaa gccgctcaca
      421 ggctatccac agcttcttag gcgtgacggt gcgcgacaac tggctggcgg aaatcgtatt
      481 ttcgctttat cccacgagat gcatggcacc tactacaact actaggtaaa taaaaatggt
      541 tctggtcaag gccaagcgag acgcaattct cgagccgctc tccgcagtca gcggaatcat
      601 cgagcgccgc cacacgctgc cgatcctctc caacgtgctc atcgagccgg gcgacggcgg
      661 cctggggttc ctcgcgaccg acatcgagat tgagataacc gcccgcgcat cgatcggcga
      721 acccggcgag gcgcgggccg tgaccgtcgg cgcgcgcaag ctggtcgata tcctgcgtgc
      781 gctgcccgag tcggccgacg tgacgctgca gcaacaggac aagcgcctgc tggtgaaagc
      841 gggcaagagc cgcttcagct tgcaaacgct gccggcggag gattttccgc gcctcgcccg
      901 gccgggtggg gagaccgcgc gtttcgggct gccgcaaaag gcgcttcgcc acctgctggg
      961 catggtgcag tacgccatgg cgcagcagga cattcgctat tacctcaacg gcctgctgat
     1021 ggtcgtggag gacgagcggc tgaagctggt tgccaccgac ggtcatcgtc ttgcatacgc
     1081 ggcgatggcg cttgagagtg cgttgccgcg ccaggaagtc atcgtgccgc gaaaaaccgt
     1141 tatcgagctc ggcaagctgc tcgccgacag cgacgatccg gtggaggtcg agctgtccgc
     1201 ggcgcaagcg gctttccgct tcggcggcgt cgaacttgtt tccaagctgg tcgacggcaa
     1261 gtttcccgat tacgcgcgtg tcattcccac gcagcacaag aatagcctgc gggtggagcg
     1321 cgaggttttg cgccagtcgc tgttgcgcgc ggcaatcctg tcgaacgaga agtttcgcgg
     1381 cgtgcgctgg gtgctgaccg attcgagcct gaaaatcgtg tcgtccaacg ccgagcagga
     1441 agaggcgcag gaggaactcg atgtgcagta cagcggagaa gcgctcgaca tcggattcaa
     1501 cgtcaactat ctgctggacg tgctgaacaa catcccgggc gcggaggtcg aatgcctgtt
     1561 cggcgatgcg gcctccagca ccctgttcca gttcgcgtcg gagcccgaat tccgctacgt
     1621 cgtaatgccg atgcggatat gaaaacgccg ctgcgaagcc gattttgttg agaaggaaga
     1681 acgatcgtgg gtgaaccgga aagcaacgcg gcattggaga cggattcgta cgggtccgaa
     1741 agcatcaagg tgctgaaggg cctcgaagcc gtccgcaagc gcccgggaat gtacatcggc
     1801 gatacctcgg acggcaccgg gctgcatcac atggtgttcg aggtagtcga caatgccgtc
     1861 gacgaggcgc tcgccggata ctgcgacgag atcgtcgtca cgattcatac cgacagctcg
     1921 atctcggttc gcgataacgg ccgcggcatt ccgactgacg tcaaggaaga cgacgaactc
     1981 aagcgctcgg cagccgaaat tgtcatgacc gaactgcacg ccggcggaaa gttcgacagc
     2041 aactcataca aggtctctgg cggattgcac ggtgtcggcg tttcggtggt caatgcgctg
     2101 tccgaatggc tgcgcctgac gatctggcgc gacgggcggg tgcacgagat cgaattcagc
     2161 aacggggtgg cgaccggacc gttgcgcgtc accggcgaca cagagaagcg aggcacggaa
     2221 gtccatttcc tcgcgtcgaa ggaggttttc ggcaacgtcg actatcatgt cgacatcctc
     2281 tcgcgccgtt tgcgtgagct ttctttcctc aacaacggcg tgcgcatcct gctcgtcgat
     2341 cagctctcga ccaaggagga aagcttcgag ttctccggcg gtgtgaaggg tttcgtggag
     2401 tacatgaacc gggcgaagaa cgttctgcat ccgaacgtgt tcgtcgccac cggtgagaag
     2461 gacggcatcg tcgccgatgt cgcaatgcag tggaacgaca cgttccagga atccgtgctc
     2521 tgtttcacca acaatattcc gcagcgcgac ggcggaacgc acatgaccgg tttgcgtgcg
     2581 gcgatgacgc gcgtcctgaa caagtacatc gaggagcacg agctcgccaa gcgcgcgaag
     2641 gtggaaacca ccggcgacga catgcgcgag ggactggcct gcgtgctgtc ggtcaaggtg
     2701 cccgagccga agttctcctc gcagacgaag gaaaagctgg tgtcgtcgga agtacggcca
     2761 gcggtcgaag aggtggttgc ggaacatctg cggcagttcc tcgacgagcg gccgaacgat
     2821 gcacgagtca ttaccgggaa gatcgtcgaa gcggcacgcg cgagagaggc cgcgcgtcgg
     2881 gcgcgcgaaa tgactcggcg caagggcgtg ctggattcga tgggcctgcc cggaaagctg
     2941 gccgactgcc aggaacgcga tcccgcgaac tgcgagctct acctggtcga gggcgattcg
     3001 gccggcgggt cggcaaagca aggacgcgac cgccgcttcc aggcgattct gccgctcaaa
     3061 ggaaagattc tgaacgtgga gaaggcgcgc ttcgacaaac tgctttcctc agccgaaatc
     3121 gcgaccatga tcacggcgct cggctgcggc ttcggcaagg acgagtacaa ccccgagaag
     3181 ctgcggtatc accgcatcat catcatgacc gatgcggacg tcgacggctc gcatatacgc
     3241 acgctgttgc tgaccttctt ctaccggcag atgcccgagc tcgtcgagcg cggccatatc
     3301 tacatcgccc agccgccgct ctacaaagcg aagctcggca agaccgaacg ctatctgaag
     3361 gacgagcatg aattgaacga gttcatgctc agacaggcac tggtggatgc ctccctcgta
     3421 gcggccgcgg gccgcgagcc gattaccggc gatgcgctat ccgagctggc gaagtcctac
     3481 ctggtttccg aggccgtcat cgaacgggcg tcgcgctgga tcgatcggga gtcactggtc
     3541 gcgctcctgc gaggcgtgca gattgatttg tcgcgccgcg agacggcaga ggcatctgcc
     3601 gaggcgctat atgccgcact gaccggcaac ggcgttcgag tgcgcgccga tttcgacggc
     3661 aagaccgatc gctaccagat ccgcatcgag cgcagctggc atggcaatct gcgcatctcg
     3721 aacattgata ccgagttcgt cgcctcgggg
//
LOCUS       DATDAA010000436         5101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_486783,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000436 DATDAA010000000
VERSION     DATDAA010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486783"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VIS73_13045"
     CDS             complement(<1..759)
                     /locus_tag="VIS73_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY5764127.1"
                     /translation="MKAIVVAPGVEAADAHSHLRQHGVVKSALLTPSIASSWQRCLDS
                     GVTAAEANCEPASGSEVREEQEKNHLLVSHALPVMETLYDQIIGTQSMVVLTNSEGYI
                     LHSLGDSDFLDRANRIALAPGVEWSEQGKGTNAIGTALVEERALVVHAHEHFVSANHF
                     LTCSASPICDPLGKVIGVLDVTGDHRSYSPHTLALVKMGVRLVENQLFSHAFPEALVL
                     HFHSRPEYLGTLAQGMMAFSPDGSVLSANHNACVQ"
     gene            1054..1482
                     /gene="pedF"
                     /locus_tag="VIS73_13050"
     CDS             1054..1482
                     /gene="pedF"
                     /locus_tag="VIS73_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-550 PedF"
                     /protein_id="HEY5764128.1"
                     /translation="MKRKLIPTMILAGLAIASTNAVAHGDVTPQAVDVSGLPPLGTVV
                     PESNPYRDNAAAIEVGTSAYNQNCARCHGLEAISGGIAPDLRKLDLDPDTDMYYYGSV
                     IAGKVRNGAVYMPPFGEILQQEAIWAIKAYLETRRASLDE"
     gene            1463..2320
                     /locus_tag="VIS73_13055"
     CDS             1463..2320
                     /locus_tag="VIS73_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY5764129.1"
                     /translation="MHRLTNKRFGRSAFALLLALASALTSPGASAEARDLDLIRENGV
                     IRFALYNDFPPYSANGKGIDYDIAEALASRLGVRFAPMWFTASDESIEDDLRNMVWKG
                     HYLGTGPADALIHAPVDPELIRRSPRVRFVAPYSRERLAVARDLKRIPRLDDLSELDG
                     MPIGVENASLASIVLLAERGGRLRDQVRHFKTPTAAIEALRKGEVAAVMAQWGETQGL
                     LGEADGYAVTPVPAIAGLGTRQWVLGIAVPKAHDSLGDALEAAMTALVADGELDRIFL
                     RHGVTRLKP"
     gene            2265..4508
                     /locus_tag="VIS73_13060"
     CDS             2265..4508
                     /locus_tag="VIS73_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY5764130.1"
                     /translation="MANSTGYSCATASPDSNPEGRTTTIAPERNTCSPDAVGARHRLH
                     ARLTRWSAACLVLALLAGALLANQASAGELTQADIERRILPPLRVAAKLPELAAWPIT
                     SELEPEAGPVAYVFESIDFAPIPGFEGTPFNLLVTLDRDGRFVDVEVLRQHEPVFLSG
                     LGEAPLLEFVRQYAGKSLHRPLSVSSAYGGKRRAGEDDTVLDGVTKATASVRILNQTV
                     LNSGLAVARARLGFGSGAARGPPAQPRMDFHVPRTTETLLDDGSIARLQLSNHEAERL
                     FQGTEAEGSDAEALRDPDGEQLTLTIAYLNPPTIGRALLGDARYAELMRALEANQPAF
                     WIATRGRTKLLAADFVPGTTPPDLVLTQDGLSLELRDFNHPVPDPPGLGPSNSAGIFV
                     VPYSAGLDPGSPMRFELGLRRAQGMILPRITKAVVALDYAPPAELFTRPPPPPPEWLL
                     AWQGRAFELAIIGAALLALSIVLARPRWISIDRQRLRRFRIAFLAFTLVFIGWIAQGQ
                     LSVVQITGAIKSLLAGGGLGSFLYDPVSLLLIAFTLVSFVVWGRGTFCGWLCPFGALQ
                     EFAVGLRERLGLREIAVRGVLARALRPLPFLTLTVLLASAAFAPAWAERLVEVEPFKT
                     AITVAFDRAWPFVAYAVVVLVTSAFVYKGFCRFLCPLGAAMALGGRLRRFDWLTRRRE
                     CGTPCQMCRRRCRYDAIETSGTIRYADCFQCLDCVGIYHDEKRCAPLVLYRKRARQGR
                     WDTLDTR"
     gene            4853..>5101
                     /locus_tag="VIS73_13065"
     CDS             4853..>5101
                     /locus_tag="VIS73_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764131.1"
                     /translation="MKKHAVGLIAGLVGGIVVSAPASAVELKGGDWTLNVNGTVNAFY
                     VNSNAKTTDVAGVTTSNKQANVQNGLLPGWIHFAATTQQ"
BASE COUNT          886 a         1785 c         1544 g          886 t
ORIGIN      
        1 ttgaacacag gcattgtggt tcgccgagag taccgaacca tcgggcgaga acgccatcat
       61 gccctgcgcc agcgttccca gatattcagg gcgcgaatgg aaatgcagca caagtgcctc
      121 gggaaatgca tgggagaaca gctgattctc gaccaagcga acacccatct tcacgagggc
      181 cagcgtatgt gggctgtagc tacggtgatc gccggtcacg tcgagcacgc cgatcacctt
      241 gcccagcggg tcgcaaattg gtgacgctga gcaggtgagg aaatggtttg ccgaaacgaa
      301 atgctcatgt gcgtggacaa ccagtgcccg ctcctcgacc agtgccgtgc cgatcgcatt
      361 ggttcccttg ccctgctcag accactcgac gcccggcgcc agcgcaattc tgttcgcccg
      421 gtcgagaaag tcggaatcgc caagtgaatg cagaatgtag ccttcggagt tggtcagcac
      481 gaccatgctc tgcgtgccga tgatctggtc gtagagcgtc tccatgaccg gcagcgcgtg
      541 gctcaccagc aggtggttct tctcctgctc ttcgcgcacc tcggacccgg atgcaggctc
      601 gcagtttgcc tcggcagcgg tgactccgct atccagacag cgctgccatg aactagcgat
      661 cgacggcgtc agcagggcgc tcttgacgac gccatgctgc ctcaaatgcg aatgcgcatc
      721 tgccgcctcg acgccaggcg ccacgacaat agccttcatt gctactcctc ctcgtgtcta
      781 cagctggctg gttgcccgta ggcaccgcct cgattcctgc tcttacgaca ggtagtcgta
      841 tgaaaccacg actaacagca tagctagcaa tacccgttcc accaacattg ctttgtggat
      901 cgtgacactt gattccgcat tacaaactat tccgggcttg cggaccggat ccgggcaaat
      961 ggcacgcaac ctgcaaaggc caatccgctg cccactggaa cccaagtgga gcccgaacac
     1021 ccgagtatgc acgaacagaa acaggagaca gcagtgaagc gaaaactaat ccccacgatg
     1081 atcctcgccg gcctcgccat tgccagcacc aacgccgttg cgcatggcga cgtcacgccg
     1141 caggccgtcg acgtcagcgg cctcccgccg ctgggcacgg tcgttcccga atccaacccc
     1201 taccgcgaca acgccgctgc gattgaagtc ggcacctcgg cctacaacca gaactgcgcg
     1261 cgctgccacg gcctcgaagc gatttccggc ggcatcgcgc ccgacctgcg caaactcgac
     1321 ctcgatcccg acaccgacat gtattactac ggctcggtga tcgccggaaa ggtccgcaac
     1381 ggtgccgtgt acatgccgcc ctttggcgag atcctgcagc aggaggcgat ctgggcgatc
     1441 aaggcctacc tggagacgcg ccgtgcatcg cttgacgaat aagcgcttcg ggcgctccgc
     1501 gttcgctctg ctgctggcgc tggcgagcgc gctgacgagc cccggcgcca gcgccgaggc
     1561 gcgagatctc gatctgatcc gcgaaaacgg cgtgatccgc ttcgcgctct acaacgactt
     1621 cccgccttat tcggccaacg gcaagggaat cgactacgac atcgccgaag cactggcgag
     1681 caggctcggc gtccgcttcg cccccatgtg gtttaccgcc tccgatgaaa gcatcgaaga
     1741 cgatctccgc aacatggtgt ggaaaggcca ctacctgggg accggcccgg ccgacgcgct
     1801 gattcacgcc ccggtcgatc ccgagctgat cagacgctcg ccgcgggtgc gctttgtcgc
     1861 cccctactcg cgcgaacgtc tcgcagtcgc ccgcgacctg aagcgcattc cgcgtctcga
     1921 cgacctctcc gaactcgacg gcatgccgat cggggtcgaa aacgcctcgc tggcctcgat
     1981 cgtcctgctc gccgaacgtg gcggtcgcct gcgcgaccag gtgcgtcatt tcaagacacc
     2041 cacggccgcg atcgaggcgc tgcgcaaggg cgaggttgcc gcggtcatgg cccaatgggg
     2101 cgagacccag ggcctgctcg gcgaggccga cggatacgcc gtaacgcccg tccccgccat
     2161 cgccggcctc gggacgcggc aatgggttct cggcatcgcc gtgcccaagg cccacgacag
     2221 cctgggcgac gcgctggaag cggcaatgac ggcgctggtt gccgatggcg aactcgaccg
     2281 gatattcctg cgccacggcg tcacccgact caaaccctga gggccggacc acgacgattg
     2341 cgccagaacg gaacacctgt tcgcccgatg cagtcggcgc ccgccaccga ctccatgcac
     2401 ggctgacgcg ctggtcggcg gcctgcctgg tccttgccct gctggcgggc gcgctgctgg
     2461 cgaaccaggc aagcgccggg gagctgacgc aggccgacat cgagcggcga atcctgccgc
     2521 cgctgcgggt tgcggcgaaa ctgcccgagc tcgcggcatg gccgatcacc agcgagctgg
     2581 aacccgaggc cggcccggtc gcctatgttt tcgaatcgat cgacttcgcg ccgattcccg
     2641 gcttcgaagg cacgccattc aacctgctgg tcacgctcga tcgtgacggt cgcttcgtcg
     2701 acgtcgaggt gctgcgccag cacgaaccgg tttttctcag cggcctcggt gaagcaccgc
     2761 tgctggaatt cgttcgccag tacgccggca agtcgctgca ccgtccgttg agcgtatcga
     2821 gcgcctacgg cggcaagcgg cgcgccggcg aagacgatac ggttctcgac ggcgtcacca
     2881 aggcgaccgc ctcggtccgc attctcaacc agaccgttct caattcgggc ctcgccgttg
     2941 cccgcgcccg cctcggcttc ggctcgggag ccgcccgtgg gccgccggcg cagccacgga
     3001 tggacttcca tgtgccgcgc acaacggaga ccttgctcga cgatggctcc atcgcccggc
     3061 tgcagctcag caatcacgag gccgagcgcc tctttcaggg aaccgaggcc gaaggcagcg
     3121 acgccgaagc gctgcgcgat cccgacggag aacagctcac actgaccatc gcctacctca
     3181 acccgccgac catcggccgg gcactgctgg gcgatgcgcg ctacgccgaa ctgatgcgcg
     3241 cgctcgaggc caaccagccg gcgttctgga tcgccacgcg cggacgcacg aaactgctcg
     3301 ccgccgattt cgttccgggc accacgcctc ccgacctagt gctgacccag gacggcctct
     3361 cgctcgagct gcgcgacttc aaccatccag tccccgaccc gccgggcctg ggtccgagca
     3421 actcggccgg catcttcgtc gttccttact ctgcggggct ggacccgggc agcccgatgc
     3481 gcttcgaact tggcttgcgc cgggcccaag gcatgatcct gccgcgcatc accaaggccg
     3541 ttgtggccct cgactacgcg ccgcccgccg aactgttcac ccgcccgccg ccgccgccgc
     3601 ccgaatggct gctggcatgg caggggcgcg cgttcgagct ggcgatcatc ggcgccgcgc
     3661 tgctggcgct gagcatcgtg ctcgcccggc cgcgatggat ttcgatcgac cgccagcgac
     3721 tgcgccgctt ccgcatcgcc tttctcgcct ttacgctggt cttcatcggc tggatcgcgc
     3781 agggacagct gtcggtggtg cagatcaccg gtgcgatcaa gtcactgctg gccggcggcg
     3841 gcctcggcag cttcctctac gatccggtca gcctgttgct gatcgccttc acgctggtca
     3901 gcttcgtggt ctggggtcgc ggcaccttct gcggatggct ctgccccttc ggcgcgctgc
     3961 aggagttcgc agtcggcctt cgcgagcgcc tcggcctgcg cgagatcgcc gtccggggtg
     4021 tcctggcgcg tgcgttgcgg ccactgccct tcctcacgct gaccgtcttg cttgcgtctg
     4081 cggcgttcgc cccggcctgg gccgagcgac tggtcgaggt cgaaccgttc aagactgcta
     4141 ttaccgttgc attcgaccgc gcctggccct ttgttgccta cgccgtagtc gtgctggtca
     4201 cgagcgcttt cgtttacaag ggattctgcc gcttcctctg cccgctcggc gcggcgatgg
     4261 cgctcggtgg ccgcctgcgc cgcttcgact ggctcacccg tcgccgcgaa tgcggaacgc
     4321 cgtgccagat gtgtcggcgg cgctgccgct acgacgccat cgagaccagc ggcacgatcc
     4381 gctacgccga ctgttttcaa tgtctggact gcgtcggcat ctaccacgac gagaagcgtt
     4441 gcgcgccgct ggtcctctac cgcaagcgtg cacgccaggg ccgctgggac accctcgaca
     4501 cgcgctgatt cccgctgaac cggcgtcaac aacggcccgc cggcggacgg gccgacgcgc
     4561 aactgcctcg cgatggcaca cctgtcattc gatcgcacag gtgatcgccc tgccactcct
     4621 gatcgcaagt cgggtcgatc gtcaccaccg aagccgcgag cgcggtcgac tgagcaattg
     4681 tggagcagtc aactgcgcca tccagacacg aattgtccag cctggaacaa cccggcgatc
     4741 gccgccggaa gcggcattta gccgatcgcc aattacgcgg ggctggcacg ttgattgccc
     4801 tgccgtgatg cgccgttttg gcgatcaaca aatccaacca tctggaggaa ctatgaaaaa
     4861 gcatgcagtc ggacttatcg ccggcctcgt cggcggaatc gtcgtctcgg cacccgcttc
     4921 ggccgtcgaa ctcaagggtg gcgactggac gctgaacgtc aacggcaccg ttaacgcttt
     4981 ctacgtcaac agcaacgcca agaccaccga tgtcgctggc gtcacgacgt cgaacaaaca
     5041 ggcgaacgtg cagaacggtc tgctgccggg ctggatccac tttgccgcga ccacgcaaca
     5101 g
//
LOCUS       DATDAA010000437         8157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_521297,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000437 DATDAA010000000
VERSION     DATDAA010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521297"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..105
                     /locus_tag="VIS73_13070"
     CDS             <1..105
                     /locus_tag="VIS73_13070"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEY5764132.1"
                     /translation="EDEAYAALRKLAMDRKRPLGDTARDVVEMAKFLL"
     gene            459..1898
                     /locus_tag="VIS73_13075"
     CDS             459..1898
                     /locus_tag="VIS73_13075"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5764133.1"
                     /translation="MKKTIDTTTESPLIALPSADQRRRDFIRRSLALSGAGVIGASGF
                     AHIPSAWAAGSEGIEKMKLKLGIIPLTDCAPLVIAKEKGYFKKHGLDVEISKEASWAN
                     IRDKVALGELDGAHMLAGMPLAATLGVGANKQDTITAFSMDLNGNGITVSNALYERMV
                     EADPEAMKKRPTTAIALKKVIDADKKAGKEPMTFAMVFPVSTHNYEIRYWMASAGIDP
                     DNDVRLIVIPPPQMVANLASNNIVGYCVGEPWNQRAVEMGIGRSIITNYEIWNNNPEK
                     VFGVTRAWAEKNPNTHLAAVRALIEAAQWMDKPENRSEVVRIIASKSYVNAPESVVEN
                     SMTGTWLYTKQEGPVQMPDFNVFYRYAATFPWRSHAVWFLTQMVRWGQVEEAINFRKV
                     AETVYRADLYREAAKDLGIAVPTIDYKTEGTNAAGWTLDKASSPIAMGPDRFFDGMRF
                     DPAKPVEYLRQFKTHSMKVALDQLAKVNA"
     gene            1955..2911
                     /gene="ntrB"
                     /locus_tag="VIS73_13080"
     CDS             1955..2911
                     /gene="ntrB"
                     /locus_tag="VIS73_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HEY5764134.1"
                     /translation="MEATLNPSSLIVPGGTSTAMSAAPAANSSAPPAAAGEAETRAGQ
                     SAEPTARHAINRKAMRTATLAASRWLIAVVAGLGALIVVWWAVSSHVSQFPGPLSTWH
                     AALELFADPFYDNGPNDQGIGWNVYESLKRVGIGFGLAAAVGIPLGFAIGRVGFLRSA
                     TDPVISLLRPVSPLAWLPIGLLVFKDANLSAIWVIFICSIWPMIINTAAGVRAMPQDY
                     LNVARVLDLPEAKIITKIYLPAVLPHILTGVRLSVGTAWLVIVAAEMLTGGIGIGFWV
                     WDEWNNLNVEHILIAIFVIGVVGLLLEQALMFVARRCSYESR"
     gene            2922..3716
                     /locus_tag="VIS73_13085"
     CDS             2922..3716
                     /locus_tag="VIS73_13085"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5764135.1"
                     /translation="MSKKIVQIEDVVQTFDTKQGSFCALQGIRLDIHAGECVSLIGHS
                     GCGKSTLLNLIAGLTSPTAGVLLCDDREIAGPGPERAVVFQNHSLLPWLTCFDNVYLA
                     VERVFGAKEGKAKLRKRTLAALELVNLAHAETKFPNEISGGMKQRVGIARALAMEPKI
                     LLMDEPFGALDALTRANLQDELMRIMADTGATTVMVTHDVDEAVLLSDRVVMLTNGPA
                     ATIGEILDVPLARPRNRLELANDPTYVSCRAAILDFLYRKQLRKAA"
     gene            complement(3877..4929)
                     /locus_tag="VIS73_13090"
     CDS             complement(3877..4929)
                     /locus_tag="VIS73_13090"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEY5764136.1"
                     /translation="MRRTSVSEAVEGCLLGCAVGDAIGLPYEGLSMGRARRFAKLPLR
                     HRFVCGRGMVSDDTDHTIFVAQALIRSRGETDVFRRVLAWRLRLWLLCLPAGIGWATL
                     RGIVRLWLGFPHSGVRSAGNGPSMRSGIIGAAVHADSVRRRALVEASTLLTHTDPRAL
                     AGALAVAEVTARLAVGAWNERPPSAELAALLNEASADPDWRRCVTAIASACDAADPIA
                     AAHEYFGGPGGVSGYVLHSVPFALVAWHHHFGDYQATLESVVLAGGDVDTVGAIAGAM
                     AGAVSGAAGIQVEWIDGIVDRPHGVDYIRMLARRLVDDSIPVATHFSPLLLPRGLAFT
                     VLVLAHGFRRLAPPYR"
     gene            complement(4926..5861)
                     /locus_tag="VIS73_13095"
     CDS             complement(4926..5861)
                     /locus_tag="VIS73_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764137.1"
                     /translation="MVSAAERKLILVTRRTRLEELVARYHSVAQARFYIEHLGADFSD
                     YVAEHDTYVAARATVSAVLEAHGRYQQIDRGFLPNFVFAADDIVLVLGQDGLLANTIK
                     YLNGQPVVGLNPDPQRWDGVLLPFEPRDLAPLLPDLLAERRDHKSVTMAEASLSDGQR
                     LLAVNDLFIGPRSHSSARYEIASGERREVQSSSGLIVSTGLGSTAWMRSIVTGSLAVA
                     AQFMPSTRQQPYTALPWDAPTLQFAVREPFPSQTSQATLVFGRINAGSALTLRSLMPE
                     NGVIFSDGIEADYVAFNSGVEAKIAVAARQGRILV"
     gene            complement(5845..6891)
                     /locus_tag="VIS73_13100"
     CDS             complement(5845..6891)
                     /locus_tag="VIS73_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY5764138.1"
                     /translation="MFGIGFIKAQPTTYLIQYRGGRAVREGVGLSFFYFSPTSSLVAV
                     PVASQDQPFIFELTTRDYQSVTIQGQVAFRVAEPHKAAALLDFSLRQDGSAYATDDPQ
                     KLKARVLAQVEVLAQQTIRGLSLQQALQADAAVVARIAAGLVEHREIASLGLEILDVS
                     VLTIKPTAETARALEAEAREAILKASDEAIYARRNAAVEQERTIRENELDTEVAVEQK
                     KRLIRETQLDAEASLRQRKAELRQADMEADITLEERRRAWVEKNAANSRTLAEAEAFR
                     VASVMTALEKADPRVVQSLAAVGMQPGQLIAQAFGGIAERAERIGQLNVSPELLQALI
                     PTAAGAENGRGQRS"
     gene            7047..7682
                     /locus_tag="VIS73_13105"
     CDS             7047..7682
                     /locus_tag="VIS73_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY5764139.1"
                     /translation="MPTQVIVTVDVVLLTLRDQRLQVALIKRPQEPFKDVLALPGGYV
                     HVDEDGDVEAAAFRVLRDKMGIRPPYLEQLASFSGARRDPRGWSLSVCHLALVAPDTV
                     ATTQWFPADKLPRLAFDHARLVAAAVTRVRNKSSYSALPGYLLPETFTLSELQAIYSQ
                     ILGEPIEKSSFRRKLRDLDFLEEIPGAFQGGRQRPAQLYRLKGMALFDRTI"
     gene            7700..>8157
                     /locus_tag="VIS73_13110"
     CDS             7700..>8157
                     /locus_tag="VIS73_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5764140.1"
                     /translation="MKSLQETTKDIVRQHYARAGIAASILSALAEAGKDVQRLTPADL
                     APVDQFHVRGRAATLELARVAGIDASMRVLDVGCGIGGTTRGLAAEFGCHVTGIDLTD
                     EYCRAAVLLSERVGLSDRVGFRQADATRLPFEAGSFDLVWTEHVAMNIAD"
BASE COUNT         1426 a         2662 c         2713 g         1356 t
ORIGIN      
        1 gaggacgagg cctatgcggc cctgcgcaag ctggccatgg accgcaagcg gccgctcggc
       61 gatacggcgc gcgatgtggt cgagatggcg aaattcctgc tctaagtttg tgcgtggcgt
      121 ctccctgttg caccgcaaaa gcgcactgcc gttgtcggca tcccgacaat cagcccatta
      181 cccgcgatgt tttcggcgcg ttgattccgg cgaaaggtgc gtcggcatgc tgtttctggt
      241 tgcaatgcgg taagggccgc tgaagctggc attgcgtttg cattagggtc agcaagcggt
      301 cggaacaatg gcgttccagt gccgtgtcag caaagcagtg ctgatggaca aaggcgtccg
      361 tctgccggga gttcctcctc tccttccggc cgacgagacg ccttttttcg ttcgagtgac
      421 cgtaaccgaa accgcccacg accccacgga gaagcaacat gaaaaagaca attgacacga
      481 cgaccgaatc accgctgatc gcacttccct cagccgatca gagacggcgc gatttcattc
      541 gccgttcact ggcgctgtca ggggccgggg tcatcggcgc ttcgggcttt gcccacatcc
      601 cgtcggcctg ggccgcgggc agcgaaggaa tcgaaaagat gaaactcaag cttggcatca
      661 tcccgctcac cgactgtgcg ccgctggtca tcgccaagga gaagggctac ttcaagaagc
      721 acggcctcga cgtggaaatt tccaaggaag cctcgtgggc gaacatccgc gacaaggtcg
      781 ccctcggcga actcgatggc gcgcacatgc tcgccggcat gccgctggcg gcgacgctgg
      841 gcgtcggcgc caacaagcag gacaccatca ccgccttctc gatggacctc aacggcaacg
      901 gcatcacggt ttccaacgcg ctctacgagc gcatggtcga agccgatccc gaggcgatga
      961 agaagcggcc gaccacggcg attgcgctga agaaggtgat cgatgccgac aagaaggccg
     1021 gcaaggagcc gatgaccttc gccatggtct tcccggtctc cacgcacaac tacgagatcc
     1081 gctactggat ggcatcggcc ggcatcgacc ccgacaacga tgtccgcctg atcgtcattc
     1141 cgccgccgca gatggttgcc aacctcgctt cgaacaatat cgtcggctac tgcgtcggcg
     1201 agccgtggaa ccagcgtgcc gttgagatgg gcatcggccg ctcgatcatc accaactacg
     1261 agatctggaa caacaatccg gaaaaggtct tcggggtgac gcgcgcctgg gccgagaaga
     1321 atcccaatac ccacctggcc gctgtgcgcg cgctgattga ggcggcgcag tggatggaca
     1381 agccggagaa ccgaagcgag gtcgtgcgca tcatcgcgtc gaagtcctac gtcaatgcgc
     1441 ccgagtcggt ggtcgagaac tcgatgaccg gaacctggct gtacacaaaa caggaaggcc
     1501 cggtgcagat gcccgacttc aatgtgttct atcgctacgc ggcgaccttc ccgtggcgtt
     1561 cgcacgcggt gtggtttctg acccagatgg tgcgctgggg gcaggtcgaa gaagcgatca
     1621 acttccgcaa ggtcgccgag acggtctacc gcgccgatct ttatcgtgag gcagccaagg
     1681 acctcggcat tgcggtacca accatcgact acaagaccga aggcacgaac gctgccggct
     1741 ggacgctcga caaggcgagc agcccgatcg cgatgggtcc cgaccgtttc tttgacggca
     1801 tgcgcttcga tccggcgaaa cccgtcgaat acctgcgcca gttcaagacg cattcgatga
     1861 aggtcgcgct cgatcagctg gcgaaggtca acgcctgact ttccgccggc gcggtcttgc
     1921 gccggccacg gattgcaaga tgaaggagat acccatggaa gccacattga accccagctc
     1981 gctgatcgtt ccgggcggaa ccagcaccgc gatgtcggcg gctccggccg caaactcgtc
     2041 ggcgccacct gccgcggcag gcgaagcgga aacgcgggcc ggacagtcag ccgaaccgac
     2101 ggcacggcac gcgatcaacc gcaaggcgat gcgaaccgcc acgctcgccg caagtcgctg
     2161 gctgatcgca gtcgtcgccg ggctcggcgc gctgatcgtc gtctggtggg cggtgtcctc
     2221 gcacgtgtcg cagtttcccg ggccgttgtc gacctggcat gcggcgctgg aactcttcgc
     2281 agaccccttc tacgacaacg gtcccaacga tcagggcatt ggctggaacg tctacgaatc
     2341 gctcaagcgc gtgggcatcg gcttcgggct cgccgccgca gtcggcatac cgcttggctt
     2401 tgcgatcggc cgcgtcggct tcctgcgtag cgcgaccgac ccggtgatca gcctgctgcg
     2461 gccggtgtcg ccgctggcct ggctgccgat cggccttctc gtgttcaagg acgccaacct
     2521 gtcggcgatc tgggtgatct tcatctgctc gatctggccg atgatcatca acaccgccgc
     2581 cggcgtgcgc gcgatgccgc aggactacct caacgtcgcc cgcgtgcttg acctgcccga
     2641 ggccaagatc atcaccaaga tctacctgcc cgccgtgctg ccgcacatcc tcactggcgt
     2701 gcgcctgtcg gtcggcaccg cgtggctggt gatcgtcgcc gccgagatgc tcactggcgg
     2761 gatcggcatc ggcttctggg tctgggacga atggaacaac ctcaacgtcg agcacatcct
     2821 gatcgcgatc ttcgtgatcg gcgtcgtcgg gttgctgctc gaacaggcgc tgatgttcgt
     2881 cgcacgccgt tgcagttacg agtcacgcta aggagaccga gatgtccaag aaaatcgtcc
     2941 agatcgagga cgtggtgcag accttcgata ccaagcaggg cagcttctgc gcgttgcagg
     3001 ggattcgcct cgacatccat gccggcgagt gcgtctcgct gatcggccac tcaggctgcg
     3061 gcaagtcgac gctactcaac ctgattgcgg ggctgacgtc gcccaccgcc ggcgttctgc
     3121 tctgcgacga tcgcgagatc gccggaccgg ggccagagcg cgccgtggtc ttccaaaacc
     3181 attcgctgct gccctggctc acctgtttcg acaacgtgta tctcgccgtc gagcgtgtct
     3241 tcggcgccaa ggagggcaag gccaagctca gaaagcgcac gctcgcggcg ctcgaactgg
     3301 tcaacctcgc gcacgccgaa accaagttcc cgaacgagat atccggcggc atgaagcagc
     3361 gcgtcggcat cgcccgggcg ctggcgatgg agccaaagat cctcttgatg gacgagccat
     3421 tcggcgcgct cgacgcgctg acccgcgcca acctgcagga tgagctgatg cggatcatgg
     3481 ccgataccgg cgcgacgacg gtgatggtga cgcacgacgt cgacgaggcc gtgctgctct
     3541 ccgaccgcgt cgtcatgttg accaacggcc cggcggcgac gatcggcgaa atcctcgatg
     3601 tgcccttggc gcggccgcgc aatcgcctgg aactcgctaa cgacccgacc tacgtgtcgt
     3661 gtcgtgcggc gatccttgat tttctctacc gcaagcagtt gcggaaagcg gcctgatcga
     3721 actcaatcgc caaggacccg atcgacgcac cgccgtcttg aacctgggcg gaaacaggcg
     3781 gtggcacgag caccgggcgt tgcccggacg agttcaagag tgaagggatc aagcaggtga
     3841 cgtcggtatt cggtcgaagc gcagggtcgg cggccgtcag cgatagggcg gcgcgagccg
     3901 gcgaaaaccg tgcgccagta ccagcaccgt gaacgccagc cctcgcggca agagcagcgg
     3961 cgaaaaatgc gttgcgactg gaatcgagtc gtcgaccagg cgccgtgcca gcatccggat
     4021 gtagtcgacg ccgtgtggac ggtcgacgat gccatcgatc cattcgacct gaatgccggc
     4081 ggcaccgctg actgcgccgg ccatggcgcc ggcgatggcg ccaaccgtgt caacgtcgcc
     4141 accggcgagg acgaccgatt cgagggtcgc ctgatagtcg ccgaagtggt ggtgccaggc
     4201 cacgagcgcg aatggcacgc tgtgcagcac gtagccggaa acgcctccgg ggccgccgaa
     4261 gtattcgtgc gccgcggcga tggggtcggc cgcatcgcag gcgctggcga ttgccgtgac
     4321 gcaacgacgc caatccggat cggccgatgc ctcgttgagc aaggccgcga gttcggcgct
     4381 cggcggtcgt tcgttccacg caccgacggc gaggcgggcg gtgacctcgg cgacggccag
     4441 cgcgccggca agtgcccgcg ggtcggtgtg tgtgagcagc gtcgacgctt cgaccagcgc
     4501 gcggcggcgc actgagtcgg cgtgcacggc cgcgccgatg atgccgctgc gcatgctcgg
     4561 accgttgccg gccgagcgca cgccgctgtg gggaaatccc agccacaggc gaacgatgcc
     4621 gcgcagcgtc gcccagccga tgccggccgg caggcacagc agccacagcc gcagccgcca
     4681 ggccagcacg cggcgaaaga cgtcggtttc cccgcgactg cgaatcagtg cctgggcgac
     4741 gaagatcgta tggtcggtgt cgtcgctgac catgccgcgg ccgcagacga agcggtggcg
     4801 cagcggcagc ttcgcgaatc ggcgcgcccg gcccatcgac agcccttcgt agggcaggcc
     4861 gatcgcgtcg ccaacggcac agccgagcag gcagccttcg accgcctcgc tgacgctggt
     4921 gcgcctcata cgagaatcct cccctggcgg gcggcgacag cgatcttcgc ctcgacgccc
     4981 gagttgaatg cgacgtaatc ggcttcgatg ccgtcgctga agatgacgcc attctcgggc
     5041 atcagcgagc gcagcgtcag cgcgctgccg gcgttgatgc gaccgaacac cagcgtcgcc
     5101 tgcgaggtct ggctcggaaa cggttcgcgc accgcgaact gcagggtcgg cgcatcccag
     5161 ggcagggccg tgtagggctg ttgtcgcgtg cttggcatga actgggcggc gaccgccagc
     5221 gagccggtga cgatgctgcg catccacgcc gtcgaaccca gcccggtgga gacgatcagg
     5281 ccgctcgatg actgcacctc gcgtcgctcg ccgctggcga tttcgtagcg cgccgagctg
     5341 tggctgcgcg ggccgatgaa gaggtcgttc accgccagca ggcgctgccc gtcggagagg
     5401 ctggcctcgg ccatcgtcac gctcttgtgg tcgcggcgct cggcgagcag gtcgggcagc
     5461 aagggcgcca agtcgcgcgg ctcgaagggc agcagcacgc cgtcccagcg ctgcgggtcg
     5521 gggttgaggc cgaccaccgg ttgcccgttg aggtacttga tcgtgttggc gagcagtccg
     5581 tcctgcccga gcaccagcac gatgtcgtcg gcggcgaaga cgaagttggg caggaagccg
     5641 cggtcgatct gctggtagcg gccgtgcgct tcgagcactg ccgataccgt ggcccgcgcc
     5701 gcgacgtagg tgtcgtgctc ggccacgtaa tcggagaagt cggcgcccag gtgctcgatg
     5761 taaaagcgcg cctgggcgac cgagtggtag cgcgccacca gctcctccag ccgggtgcgc
     5821 cgggtgacca ggatcagctt gcgctcagct gcgctgacca cggccgttct ccgcgccggc
     5881 cgctgtcggg atcagcgcct gcagcagctc cggcgagacg ttgagctggc cgatgcgctc
     5941 ggcccgctcg gcgatgccgc cgaaggcctg ggcgatcagc tggcccggct gcatgccgac
     6001 tgcggcaagc gactgcacga cgcgcgggtc cgccttctcc agcgccgtca tgaccgaggc
     6061 aacgcggaag gcctcggcct cggccagcgt ccggctgttg gcggcattct tctcgaccca
     6121 ggcccggcga cgttcctcga gcgtgatgtc ggcctccatg tcggcctggc gaagctcggc
     6181 cttgcgctgc cggaggctgg cctcggcgtc gagctgggtc tcgcggatca gccgcttctt
     6241 ctgctcgacg gcgacctcgg tatcgagttc gttctcgcgg atggtgcgtt cctgttcgac
     6301 cgctgcattg cgccgcgcgt aaatcgcctc gtcggaggcc ttgaggatcg cctcgcgcgc
     6361 ctcggcttcc agcgcgcggg cagtctcggc ggtcggcttg atggtcagca ccgagacgtc
     6421 gaggatctcc aggcccagcg aggcaatctc gcggtgttcg acgaggccgg cggcgatccg
     6481 cgcgaccacg gccgcgtctg cctgcagtgc ctgctgcaag ctcaggccgc gaatcgtctg
     6541 ttgtgccagc acttcgacct gcgccagcac ccgcgccttg agtttctgcg ggtcgtccgt
     6601 ggcataggcc gagccgtcct ggcggaggct gaagtcgagc agcgccgcgg ccttgtgcgg
     6661 ctcggcgacg cggaaggcga cctggccctg gatcgtgacc gactggtagt cgcgcgtggt
     6721 cagttcgaag atgaacggct ggtcctggct ggccaccggg acggctacca gcgacgaggt
     6781 gggcgagaag tagaagaagg aaagcccgac gccctcgcgc accgcgcgac cgccgcggta
     6841 ctggatcagg taggtcgtcg gttgcgcctt gatgaagccg attccgaaca tgttgcacct
     6901 ccgttctctg ggcctttggg tggccccgtc tgctgcgctt gtttatatag tggcgaaacg
     6961 ccacgaacac agtacagcac acatagtggc aaagagcaac taaaaagatt aggattcagg
     7021 caaaccactt ccggaagcca acgggcatgc ccacgcaggt aatcgtcacc gtcgatgtcg
     7081 tactgctgac gctgcgcgac cagcggctgc aggtggcatt gatcaagcgg ccgcaggaac
     7141 ccttcaagga cgtgctggcg ctgcccggtg gctacgtcca cgtcgacgag gacggcgacg
     7201 tcgaggcggc cgccttccgc gtcctgcgcg acaagatggg aatccggccg ccctacctcg
     7261 agcagctggc cagtttctcg ggagcacgtc gcgatccgcg cggctggtcg ctgagcgtct
     7321 gccacctggc gctggttgca cccgacacgg tggcgacgac ccagtggttc ccggccgaca
     7381 agctcccgcg gctcgccttc gaccacgcgc ggctcgtcgc cgccgccgtc acccgggtac
     7441 gcaacaagtc gtcgtattcg gcgttgccgg gctacctgtt gccggagacg ttcacgctgt
     7501 cggaactgca ggccatctac agccagatcc tcggcgagcc aatcgagaaa agctcgttcc
     7561 gccgcaagct gcgcgacctc gatttcctgg aagagatacc cggcgccttc cagggagggc
     7621 gtcagcgccc cgcgcagctg taccggctca agggcatggc actgttcgac cggacgatct
     7681 gatccgcgaa aggcatcggg tgaaaagcct gcaagagaca accaaggaca tcgttcggca
     7741 acactacgcg cgggccggca tcgccgcttc gatcctgtcg gcgctggcgg aagccggcaa
     7801 ggatgtgcag cggctgactc cggccgatct ggcgccggtg gatcagtttc acgttcgcgg
     7861 acgggcagcg acgctggagc tggcgcgggt ggccgggatc gacgcctcga tgcgcgtgct
     7921 cgacgtcggc tgcggcatcg gtggcaccac gcgcgggctt gccgctgagt tcggctgcca
     7981 cgtcaccggc atcgacctga ccgacgaata ctgccgcgcc gcggtgctgc tgagcgagcg
     8041 ggtgggcttg agcgaccgcg tcggatttcg ccaggccgac gccacgcgat tgcctttcga
     8101 ggcaggcagc ttcgatctgg tgtggaccga gcacgtggcg atgaacatcg ccgacaa
//
LOCUS       DATDAA010000438         3286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_172436,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000438 DATDAA010000000
VERSION     DATDAA010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172436"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VIS73_13115"
     CDS             <1..435
                     /locus_tag="VIS73_13115"
                     /inference="COORDINATES: protein motif:HMM:NF023282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HEY5764141.1"
                     /translation="TQLNNVLGGTAEGTENVIWDARLRQTTTAWGVTLRGRPLTRLAV
                     AAELSRSTDKAEHDLAQTGGTGTIGPSSLPDYRYRTTTLKLSGDYTLDRYSGVQVEII
                     ADQRENNDWTWPGWTYSDGTMVINPADETAVFAGVRYRYRWR"
     gene            complement(509..2215)
                     /locus_tag="VIS73_13120"
     CDS             complement(509..2215)
                     /locus_tag="VIS73_13120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5764142.1"
                     /translation="MSGIFNSIGQRLLLMVSAVVGIGMIGLVLIYTDRQEATIFRENE
                     QALQKVSDSVAEGLSALMLKGHAKAAEEFAQRLKTVPNIIDYRIIRPDGTEAFVDNAT
                     VDSVNDRLGDLEFFGRKGSPKPIAVLAPTDPDLVRARKEERRVISYETMPGGERLVTI
                     LGPIRAQEGCAACHDQSTGVRGFIKLTTSLKDIDADIQRTRNLAIVFIAVGLLLIVTA
                     IYWFARRTVVDHIININKAMATAATGDLSQRLSEKKGDEIGFMARSFNRMSDELLQIY
                     AGLRDERSKLTTIIRGADSGIVVTDSEQQIVLVNPSACKLLGKDEQQVVRDGLMATFD
                     DPDWLAAEFNRQAWDPKPAMLRRGDRILSIQASVIRDAGGAMIGSAILIRDVTREKAL
                     EETLRRESITDALTGLYNRRHFDETILAEFKRWERYQQPFALIMVDIDHFKKFNDTHG
                     HECGDRVLEAIGKVLGNISTHPCVACRYGGEELVVIMPGSRESIAAGLAEALRQQIAA
                     LVIDGMGVTASFGVAGVPGHRIEDGNALLKLADDALYRAKESGRNRVCVAPAEGADEA
                     PA"
     gene            complement(2212..>3286)
                     /locus_tag="VIS73_13125"
     CDS             complement(2212..>3286)
                     /locus_tag="VIS73_13125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5764143.1"
                     /translation="LAALVLIIVLTRYVLRRYIVTPIERVSEAMAKVSTGDLYQEVPV
                     FGSDELAVMARNFNKMTSDLRESYLGFNSEHNKLETIIMGTQEGIIATNGEGEIVLLN
                     PAAERLLEKSSDEILAGGFLNLLDDSARMEALLGREGRASERHDIVLYRRRVLDVLAT
                     AIHSSSGNLIGHTAILRDITQEKLMEQKLRDLSSVDPLTGLANRRRLNEVLAAEFELS
                     QRQKRPMSILMFDVDHFKRFNDEHGHDQGDRVLQAVAAATRECVRDVLDTVCRYGGEE
                     FLVIARETPQSGGVILAERVRSAVEEMRVDGLSVTISIGVAGLHERNAKSPESLIEGA
                     DAALYEAKRAGRNRVMAAPVVRS"
BASE COUNT          582 a         1068 c         1034 g          602 t
ORIGIN      
        1 actcagttga acaacgtctt gggcggaacc gcggaaggta ccgagaacgt tatctgggac
       61 gcccgcctca gacagaccac gaccgcctgg ggtgtcacgc tgcgtggtcg gccactgacc
      121 agactggcgg tcgccgccga actcagccgg tcgaccgaca aggccgagca cgacttggca
      181 cagaccggtg gcaccggaac catcgggccc tcatcactgc ccgactaccg ctaccgcacg
      241 acgacgctga agctctccgg cgattacacg ctggaccgtt acagcggcgt gcaagtggaa
      301 atcatcgctg accagcgtga gaataacgac tggacttggc cgggctggac ctacagcgac
      361 ggcacgatgg tcatcaaccc ggccgatgaa accgccgtct tcgccggcgt gcgctaccgc
      421 taccgctggc ggtaggaaac cgacgcaaag cgaaacgggc gcccttgggc gcccgttgct
      481 ttttcgcggc cgctgactac cgccggattc aggccggcgc ctcgtccgcg ccctcggctg
      541 gggcgacgca gacacggttg cgcccgctct ccttggcgcg gtagagcgca tcgtcggcga
      601 gtttaagcag ggcattgccg tcttcgatac ggtggcccgg cacgccagca acgccgaagc
      661 tcgccgtgac tcccatgcca tcgatgacca gcgccgcgat ctgctggcgc aacgcctcgg
      721 cgagtccggc ggcgatgctt tcccggctgc cgggcatgat caccacgagt tcctcgccac
      781 cgtaacggca ggcgacgcag gggtgcgtgc tgatgttgcc gagcaccttg ccgatcgcct
      841 ccagcacacg gtcaccgcat tcgtggccgt gcgtgtcgtt gaatttcttg aagtggtcga
      901 tatcgaccat gatcagggcg aacggctgct gatagcgctc ccagcgcttg aactcggcga
      961 ggatcgtttc gtcgaaatgg cgccggttgt agaggccggt caaggcgtcg gtgatcgatt
     1021 cgcggcgcaa cgtctcctca agcgccttct cccgcgtgac atcgcggatc aggatagccg
     1081 acccgatcat cgcgccgccc gcgtcacgga tcaccgatgc ctgaatcgac aagatgcggt
     1141 cgccacgccg gagcatcgcc ggcttcggat cccaagcctg ccggttaaac tcggcagcca
     1201 gccagtctgg gtcgtcgaag gttgccatca ggccatcgcg gaccacctgt tgctcgtcct
     1261 ttccgagcag cttgcacgcc gacgggttga ccagaacgat ctgctgttcg gaatcggtga
     1321 cgacgatccc cgaatcggcg ccgcgaatga tggtcgtcag cttgctgcgt tcgtcgcgca
     1381 ggccggcgta gatctgcagc agctcatcgc tcatgcggtt gaaggagcgc gccatgaagc
     1441 cgatctcgtc gcccttcttc tccgacagcc gctgcgagag gtcgccggtt gcggcagtcg
     1501 ccatcgcctt attgatgtta atgatgtgat cgacaacggt gcgccgcgcg aaccagtaga
     1561 tggcggtgac gatcagcagc agtccgacgg cgatgaagac aatggcgaga tttcgcgtgc
     1621 gctggatgtc ggcgtcgatg tccttgagcg aggtagtcag ctttataaat ccgcgaacgc
     1681 cggtgctctg gtcgtggcag gctgcacacc cctcctgggc gcgaatcggg ccgaggatgg
     1741 tcaccaggcg ctcgccgccg ggcatcgtct cgtaggaaat gacgcgccgc tcttccttgc
     1801 gggcacgaac gagatcggga tcggtcggcg ccagaacggc gatcggcttg ggtgagccct
     1861 tgcgtccgaa gaactccaag tcgccgagcc ggtcgttgac cgaatcgacg gtggcgttgt
     1921 cgacgaatgc ctcagtgccg tcggggcgga tgatccgata gtcgatgatg ttgggtaccg
     1981 tcttcagccg ctgggcaaac tcctcggcgg ccttggcgtg acccttgagc atcagcgccg
     2041 agaggccctc ggcgacgctg tcagaaacct tctgcagcgc ctgctcgttc tcccggaaga
     2101 tcgtcgcttc ctggcggtcg gtgtagatca gaaccaggcc gatcatgccg atgccgacca
     2161 cggcgctgac catcagcagc aggcgctgac cgatgctgtt gaagatgccg ctcatgagcg
     2221 caccaccggc gccgccatca cgcgattgcg acccgcccgc ttcgcttcgt agagggccgc
     2281 atcggcacct tcaatcagcg attccggcga cttggcgttg cgctcgtgca agccggcaac
     2341 gccgatgctg atggtcacgc tcaggccatc gacgcgcatc tcctcgaccg cgctgcgaac
     2401 ccgctcggcg aggatcacgc ccccgctttg cggcgtttct cgggcaatca cgaggaattc
     2461 ctcgccgcca taacgacaga ccgtatcgag cacatcgcga acgcactcgc gcgtcgcggc
     2521 ggcgacggcc tgcaggaccc ggtcaccctg gtcgtgacca tgctcgtcat tgaagcgctt
     2581 gaagtggtcg acgtcgaaca tcaggatcga catcggccgc ttctgccgct gcgacagctc
     2641 gaactcggcc gcgagcacct cgttgaggcg gcggcggttg gccagtccgg tcagcggatc
     2701 gacgctcgag agatcgcgca gcttctgctc catcagtttt tcctgcgtga tgtcgcgcag
     2761 gatcgccgtg tgcccgatca ggttgccgct tgaggaatgg atcgccgtcg ccagcacgtc
     2821 gagcacgcgc cggcggtaga ggacgatgtc gtgccgctcg gaagccctcc cctcgcgacc
     2881 gagcagcgct tccatgcggg cactgtcgtc gagcaggtta agaaagccgc cggccagaat
     2941 ttcgtccgag ctcttctcga gcaggcgctc ggccgccgga ttgagcagaa caatctcccc
     3001 ttcgccattg gtggcaatga tgccttcctg cgtacccatg atgatcgtct cgagcttgtt
     3061 gtgctcggag ttgaagccga gatacgactc gcgcaggtcg ctggtcatct tgttgaagtt
     3121 gcgtgccatc accgccagct cgtcagagcc gaacaccggc acttcctggt aaaggtcgcc
     3181 ggtcgacacc ttggccatcg cctcggaaac ccgttcgatc ggcgtcacga tgtagcggcg
     3241 caggacgtag cgcgtcagga cgatgatcaa taccagcgct gcaagt
//
LOCUS       DATDAA010000439         2725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_172489,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000439 DATDAA010000000
VERSION     DATDAA010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172489"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="VIS73_13130"
     CDS             complement(<1..1330)
                     /locus_tag="VIS73_13130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY5764144.1"
                     /translation="MKPEAPNGAPANPPGALPQNRDIWAIAAPMILSNVSVPLLGMVD
                     TGVTGHLEDAVYLGAVAIGSAIFGFLYAGFNFLRMGTTGIVAQRFGARDDDGLKVALG
                     QALIVATGIALALILLQAPVAALSLELLGPDAGIAPFARQYFDIRIWSAPATLANYAL
                     IGWFLGLQNARVPLLIVLVINLTNIALDLLFVLGLGMKVGGVALASVIAEFAGRAVGM
                     SRAADWLRRRPGRVRRRHLTSLPEYRAFFAVNAHLLVRTMALIFSFTFVTAAGARLGG
                     LFLAANAVLMNMVNLMAYALDGFAHAAEALVGKAVGARDGPALRRSVTLALRWSLGVA
                     AMFSAAFLVGGAAIIRLLTDLPEVIDTSLEYLPWVAALPLIGCWSYLYDGVFVGATRA
                     REMRDIMLISSFCVFVPAWYAFRFLGNHGLWLALTLFLAARGVGMHYHYRN"
     gene            complement(1332..2510)
                     /locus_tag="VIS73_13135"
     CDS             complement(1332..2510)
                     /locus_tag="VIS73_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5764145.1"
                     /translation="MAAADKRTLNPLDLYDIRSELTEEECMVQDSVARMVNEKVLPII
                     QEHFEKHTFPQELVREVADLGLLGSSIDGYDCAGLNAISYGLICQELERGDSGLRSFV
                     SVQSSLVMYPIHAYGSDEQKERWLPAMARGEAIGCFGLTEPQGGSDPANMKTHAKRDG
                     DDWIINGSKMWITNGNLAHAAVVWAMTDDGVRGFIVETDTPGFTAQEIENKFSLRASV
                     TSALFLDNVRVPKTNMLPGVVGLKGPLSCLTQARYGITWGVIGAAQACLGEVLDYTRD
                     RVLFGRPLSHTQTIQIRMADMARRITAAQLLSLRLGRMKDAGTMTPTHVSVAKWNNCR
                     AALDIARDARDILGGSGISAEYVPIRHMLNLESVITYEGTETVHQLVVGKELTGVNAF
                     "
BASE COUNT          507 a          888 c          892 g          438 t
ORIGIN      
        1 ggttccggta gtggtagtgc atgcccacgc cgcgggctgc gaggaacagc gtcagcgcaa
       61 gccagaggcc gtggttaccg agaaagcgga acgcgtacca ggcgggcacg aacacgcaga
      121 aggacgaaat cagcatgatg tcacgcattt cccgcgcccg cgtcgcgccg acgaagaccc
      181 cgtcgtagag ataagaccag cagcctatca gcggcagcgc cgccacccag ggcaggtatt
      241 ccagcgacgt gtcgatgacc tccggcaggt cggtcaggag ccggatgatc gccgcgccgc
      301 ccaccaggaa tgccgcggag aacatcgccg cgacgccgag cgaccagcgc agggccagcg
      361 tgaccgagcg cctcaacgcg ggcccgtcgc gcgcgccgac cgccttgccg accagggcct
      421 ccgcggcatg cgcgaagccg tccagcgcat aggccatcag gttgaccatg ttcatgagca
      481 cggcgttggc cgcgaggaac agtccgccca gcctggcgcc ggcggccgtg acgaaggtga
      541 atgaaaagat cagcgccatg gtgcggacca gcaggtgggc attgaccgcg aagaaggccc
      601 tgtactcagg gagcgacgtc aggtgccggc gccgcacccg gcccgggcga cggcgcagcc
      661 agtcggcggc ccggctcatg ccgaccgccc ggccggcaaa ttccgcgatc accgacgcca
      721 gggccacgcc accgaccttc atgcccaggc cgagcacgaa cagcaggtcg agagcgatat
      781 tggtgagatt gatgacgagc acgatcagga gcggcacccg cgcattctgc aggcccagga
      841 accagccgat cagtgcgtag ttggcgagcg ttgcgggggc gctccatatg cgaatgtcga
      901 agtactgcct ggcgaacggg gcgatcccgg catcgggccc gaggagttcg agcgacaagg
      961 cggcgacggg agcctgcaac agtatcagcg ccagagcgat ccccgtcgcc acgatcaggg
     1021 cctgcccgag cgccactttc aggccgtcgt cgtcgcgcgc gccgaatcgc tgcgccacga
     1081 tcccggtggt gcccatgcgc aggaaattga agccggcgta gaggaagccg aatatcgccg
     1141 agccgattgc gaccgcgccg agatagaccg cgtcctcgag atggcccgtc accccggtat
     1201 cgaccatgcc gagcagcggc accgagacgt tggacaggat catcggcgcc gcaatggccc
     1261 agatgtcgcg gttctgcggc agcgcgcccg gcggattggc gggcgcgccg ttcggcgcct
     1321 ccggcttcaa atcagaaggc gttgacgccc gtgagctcct tgccgaccac gagctggtgg
     1381 acggtctcag tgccctcgta ggtgatgacg ctctcgagat tgagcatgtg ccgaatcggg
     1441 acgtattcgg cgctgatgcc ggagccgccc aggatgtctc tcgcgtcgcg cgcgatatca
     1501 agggccgcgc ggcaattgtt ccacttcgcg accgatacgt gggtcggcgt catggtgccc
     1561 gcatccttca tccgcccgag cctcagcgac agcagctgag ccgcggtgat gcgcctcgcc
     1621 atgtcggcca tgcggatctg gatcgtctgc gtgtgcgaca agggccggcc gaacaggacg
     1681 cgatcccggg tgtaatcgag gacttcgccc aggcaggcct gcgcggcgcc gatcaccccc
     1741 caggtaatgc cgtagcgagc ctgggtcagg cagctcagcg ggcccttcag cccgaccacc
     1801 ccgggcagca tgtttgtctt cggcacgcgg acgttgtcga gaaacagcgc cgaggtaacc
     1861 gacgcgcgca gcgagaactt gttttcgatc tcctgggccg tgaaaccggg agtgtccgtt
     1921 tcgacgatga atccgcggac gccatcgtcg gtcatggccc agactaccgc cgcgtgcgcg
     1981 aggttgccgt tggtgatcca catcttggag ccgttgatga tccagtcgtc gccgtcgcgt
     2041 tttgcgtgcg tcttcatgtt cgccgggtcg gagccgccct gcggctcggt cagtccgaag
     2101 cagccgatcg cctcgccacg cgccatcgcc ggcagccagc gctccttctg ctcgtccgag
     2161 ccataggcgt ggatcgggta catcacgagg ctggactgca ccgacacgaa gctccttagc
     2221 ccgctgtcgc cgcgctcgag ttcctggcag atcagcccgt aggagatggc gttgagtccc
     2281 gcgcagtcgt agccgtcgat cgaactcccg agcagcccga gatcggcaac ctcacggacg
     2341 agctcttgtg gaaacgtatg cttctcgaag tgctcctgga tgataggcag gaccttttcg
     2401 ttcaccatcc gggcgaccga gtcctgcacc atgcattctt cttccgtcag ttccgaacga
     2461 atatcgtaaa gatcgagagg attgagtgtt ctcttgtcag cggcggccac ggtttctccc
     2521 cggcgtggtc aggaatataa cgagggctga ttctacccgc aggcgttcgt gccggacagc
     2581 cccgaaacag gattcgatcc cgtcatgcga tcaaaatgga atgccgattt ttcgatacag
     2641 gttggacagc agccagatcg gcgcgattat catgagctgg atgtcgtgaa aaacggcgcg
     2701 catgcccccg cggccgcgat gaccc
//
LOCUS       DATDAA010000440         3268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_207587,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000440 DATDAA010000000
VERSION     DATDAA010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207587"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /gene="uvrC"
                     /locus_tag="VIS73_13140"
     CDS             <1..1334
                     /gene="uvrC"
                     /locus_tag="VIS73_13140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY5764146.1"
                     /translation="ANRSRPCLLYQIRRCSAPCTGAIAADAYLDDVRMAKLFLRGQGQ
                     EVIRILTARMEEAAAALAFEQAARFRDQIQSLGQMRTRQHVTGIVDADTDVVVALEQD
                     GMLGINLAMIRAGEHLGDRPMFPTHGQHATPLEVLVSFVRQHYLKHPAPQRLLCNLEL
                     PAEVIGDLAVIAAEPIAVAAPRGDSQRSWVAQAKANLHLAFAARSGTQAQQLARLETL
                     RDALGLDDLPRRIECFDISHTQGERPVASCVVYREQGMSRRDYRRFNIADIKPGDDYA
                     AIAQAVRRRYDGLADGEGAPPDLVLIDGGLGQVNAARAALAEIGLADLPLVGIAKGEG
                     RKPGLEKLVPADGRQPIQLAPDSAGLHLLQQIRDEAHRFAIAGHRARRGKARRESALD
                     DVAGVGPKRRKLLIAHFGSVQGLREATTERIAQVPGIGAALADDIYKALHS"
     gene            1331..1927
                     /gene="pgsA"
                     /locus_tag="VIS73_13145"
     CDS             1331..1927
                     /gene="pgsA"
                     /locus_tag="VIS73_13145"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY5764147.1"
                     /translation="MIFNLPTSLTWVRVALVPALFVLFLLPASMIEPTVRDITATAMF
                     VAAAVTDWLDGYLARRLGQESAFGAFLDPVADKLMVAAALIILVALGRVEAVVAGIVI
                     GREITISALREWMAELGARASVAVSMVGKVKTVAQMVAISFLLYDAPLWGLPIHELGT
                     ALVYLAVVLTLWSMAYYLRCAWPLLKAGGRVKGKAADE"
     gene            1985..>3268
                     /locus_tag="VIS73_13150"
     CDS             1985..>3268
                     /locus_tag="VIS73_13150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="HEY5764148.1"
                     /translation="MCGIAGELRFDGAAPERAVLECMGERLARRGPDAHGYWAKDTVA
                     FIHRRLAIIDLSERSAQPMVDEAAQLALVFNGTIYNYPELRTALQGLGHDFRSDGDTE
                     VILRAYAEWGEACVERLHGAFAFAIWDGGRQQLFLARDRIGIKPLYYALDAQSLRFAS
                     SPQALLAGGGIDTRLDLAGLHNHFSLHGVVLPPRTILAGIRKLAQATTLTLDHSGKTR
                     SRRYWTFNAERPELPHNEHQWTEMVHAALRNATRKRLEIADVKVGVLLSGGLDSSLLV
                     ALLAEQGVADLMTFSIGFEDQPEEKGNEFEFSDAVAAYFGTRHHRYQIGNDEVLSRLP
                     EVVECMAEPMASQDVAAFYLLSERVSKTVKVVQSGQGADEVFAGYFWHQRMAEDKQGT
                     DLERFRRHYVDRDHSEFADMLADDFHGEDVTTPMLE"
BASE COUNT          526 a         1095 c         1078 g          569 t
ORIGIN      
        1 ttgccaaccg ctcgcgtccc tgccttctct accagattcg ccgctgcagc gcgccgtgca
       61 ccggagccat cgctgccgat gcctacctcg acgacgtgcg gatggccaag ctattcctgc
      121 gcgggcaggg acaggaagtg atccgcatcc tcaccgcgcg catggaggag gctgccgcag
      181 cgctggcctt cgagcaggcc gcgcgcttcc gagaccagat tcagtcgctt ggccagatgc
      241 gcacgcgcca gcacgtcacc ggcatcgttg acgccgatac cgatgtcgtc gttgcgctcg
      301 agcaggacgg gatgctcggc atcaatctgg cgatgatccg cgccggcgag catcttggcg
      361 accggccgat gttccccacg cacggccagc atgctacgcc gctcgaggtg ctcgtctcct
      421 tcgtccgcca gcactacctc aagcatccgg cgccgcagcg actgctctgc aatctcgaac
      481 tgccggctga ggtgattggc gatctcgcag tcattgccgc cgagccgatc gccgtggcgg
      541 cgccgcgcgg tgattcgcag cggtcctggg tggcgcaggc caaagccaac ctccatttgg
      601 ccttcgccgc ccgcagcggc acgcaggcgc agcagctggc gcgccttgaa acgctgcgcg
      661 atgcgctcgg actcgacgat ctgccccggc gaatcgaatg ctttgacatc agccacacgc
      721 agggcgagcg gccggtggct tcgtgcgtgg tctaccgtga acagggcatg tcgcgcaggg
      781 actaccggcg tttcaacatc gccgacatca agcccggcga cgactacgcg gcgatcgccc
      841 aggccgttcg ccgccgctac gacggcctcg ccgatggcga gggtgccccc cccgatctgg
      901 tgctgattga cggcgggctg gggcaagtca atgcggcgcg ggcggcattg gccgagatcg
      961 gcctcgccga tctgccgctg gtcggcatcg ccaaggggga gggccgaaag cccggtctgg
     1021 aaaagcttgt acctgcggat ggccggcaac cgatacaatt ggccccagac agcgcgggcc
     1081 tgcacctgtt gcagcagatt cgcgacgagg cgcaccgttt cgccatcgcc gggcatcggg
     1141 cccgacgcgg caaggcgcgc cgcgagtcgg cgctcgacga tgttgccggc gtcggtccca
     1201 agcggcgcaa gctgctgatt gcccatttcg gcagcgtgca gggcctgcgc gaggcgacaa
     1261 ccgagcggat cgcgcaggtg ccgggaatcg gtgccgcgct ggcagacgat atctacaagg
     1321 cgcttcactc atgatcttca acctgccgac atcgctgacc tgggtccggg tcgcgctcgt
     1381 gccggcactc tttgtgctgt tcctgctgcc ggcgtcgatg atcgaaccga cggttcgcga
     1441 catcaccgcc accgcgatgt tcgtcgccgc cgcggtgacc gactggctag acggttacct
     1501 ggctcgccgc ctcggtcagg aatcggcgtt tggtgccttt ctcgaccccg tcgccgacaa
     1561 gctcatggtc gccgcggcgc tgatcattct ggtcgcgctg ggacgcgtcg aagccgttgt
     1621 cgccggcatc gtcatcggcc gtgagatcac gatttcggcg ttgcgcgaat ggatggccga
     1681 actcggtgct cgcgccagcg tggccgtgtc gatggtcggc aaggtcaaga ccgtcgcgca
     1741 aatggtggcg atttccttcc tgctctacga cgcgccactg tggggcctgc cgatccatga
     1801 actgggcacg gcgctggtct atctcgccgt cgtgctgacg ctctggtcga tggcctatta
     1861 cctgcgctgc gcctggcccc tgctcaaggc gggtggacgc gtcaagggca aggccgccga
     1921 tgaatgagat ccgcggtgtc cggccgcagc ttgctgcggt gcaccgcgca ttcaccccta
     1981 gatcatgtgc ggcatagccg gcgaactgcg tttcgacggg gcggcacccg agcgcgccgt
     2041 gctcgagtgc atgggcgagc ggctggcgag acgcggtccc gacgcgcacg ggtactgggc
     2101 gaaggacacg gttgccttca tccatcgccg actggcgatc atcgacctct ccgaacgctc
     2161 ggcgcagccg atggtcgacg aggcagcgca gctggcgctg gtctttaacg gcaccatcta
     2221 caactacccg gaactgcgca cggcgctgca ggggctgggc cacgactttc gctccgacgg
     2281 tgataccgag gtcatcctgc gtgcctacgc cgaatgggga gaagcctgcg tcgaaagact
     2341 gcacggagcc tttgccttcg cgatctggga cggcgggcgc cagcagctct ttcttgctcg
     2401 tgaccggatc ggcatcaagc cgctctacta cgcgctcgat gcgcagtcgc ttcgcttcgc
     2461 ctcctcgccg caggcactgc tcgccggcgg cggcatcgac acgaggctcg atctggccgg
     2521 gctgcacaat catttcagcc tgcacggcgt cgtcttgccg ccgcgaacga ttctcgcggg
     2581 catccgcaag ctggcgcagg cgacgacgct gacgctggac cactcgggca agacgcgaag
     2641 ccgtcgctac tggaccttca atgccgagcg ccccgagctg ccgcacaacg agcatcagtg
     2701 gaccgagatg gtccatgccg cactgcgcaa cgcaacgcga aagcgactcg agatcgccga
     2761 cgtcaaggtc ggcgttctgc tctcgggggg actcgattcg tcgctcttgg tggcgctgct
     2821 ggccgagcag ggcgtcgccg acctgatgac cttttcgatc ggctttgagg accagcccga
     2881 ggaaaagggc aacgagtttg agttctccga tgccgtcgcc gcctacttcg ggacgcgcca
     2941 ccatcgttac cagatcggca acgacgaggt gctgtcgcgg ctgcccgaag tcgtcgagtg
     3001 catggccgag ccgatggcga gccaggatgt cgccgccttc tacctgcttt ccgagcgggt
     3061 ttcaaagacg gtcaaggtgg tgcagagcgg gcagggcgcc gacgaggtgt tcgccggcta
     3121 cttctggcat cagcgcatgg cagaggacaa gcagggcacc gacctcgagc gcttccgccg
     3181 gcattacgtc gatcgcgatc acagcgagtt cgccgacatg ctcgccgacg acttccacgg
     3241 tgaggatgtg acgacgccca tgctcgag
//
LOCUS       DATDAA010000441         2771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_347207,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000441 DATDAA010000000
VERSION     DATDAA010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347207"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VIS73_13155"
     CDS             <1..545
                     /locus_tag="VIS73_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY5764149.1"
                     /translation="GMRVLVERCLDGQGQTESTFLERGVESFRRHYARSNGESARLFP
                     GAREGLDALRAAGLPLACVTNKPAEFTIPLLERLGIAEYFSAVVAGDTLPYKKPRPEP
                     IRHACAQIGVSATENVHIGDSLHDCEAAHAAGCCYFHVPYGYADPAQPVPVAIGKCDA
                     LVSDLVDAASRVGAINQGQH"
     gene            761..2248
                     /gene="trpE"
                     /locus_tag="VIS73_13160"
     CDS             761..2248
                     /gene="trpE"
                     /locus_tag="VIS73_13160"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEY5764150.1"
                     /translation="MTETEFNALAAQGYNRIPVTLETFADLDTPLSIYLKLAGGPYSY
                     LLESVQGGERFGRYSFIGLSSPTRIAVYGHDVMVLTGHRISEREQDTNPLDFIGKYLS
                     RFRAAPMAGLPRFCGGLVGYFGYDTVRYIETRLTRTGKNGAGAGDLGTPDILLLLSEE
                     IAVVDNLSGKLTLVVYAEPGVPGAYGKARQRLRELLGRLREPAKIPDETPHKAPAAAS
                     VFGEAAFKKAVLKAKQYITDGDIMQVVLSQRMSKAYGATPLALYRALRTLNPSPYMFY
                     FDFEDFQVVGASPEILVRLEGDAVTVRPIAGTRRRGASAEEDQELADELLGDEKERAE
                     HVQLLDLGRNDIGRVAQTGSVRVTENMLVERYSHVMHIVSNVEGRLLPGLDALAVLKA
                     TFPAGTVSGAPKVRAMEIIDELEPVKRGVYAGAVGYLGFHGDMDVAIAIRTGVVKDGE
                     LHVQAGAGIVADSDPAAEWQETQSKARALLRAAEMAEDGLDTSLE"
     gene            2340..>2771
                     /locus_tag="VIS73_13165"
     CDS             2340..>2771
                     /locus_tag="VIS73_13165"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004938990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY5764151.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGQRVEVYRNDAIGVAEIARLKP
                     AYLVVSPGPCAPKQAGISLAAIREYAGKIPLLGVCLGHQAIGEAFGGKIVHARRLMHG
                     KVSPVTHTGKGVFAGLPNPLTCARYHSLAIERASLPDCLEIT"
BASE COUNT          470 a          946 c          861 g          494 t
ORIGIN      
        1 agggcatgcg cgttctcgtc gagcgctgcc tcgacggtca ggggcagacc gaaagcactt
       61 tcctcgagcg cggcgtcgaa tccttccgcc gtcactacgc gcgtagcaac ggggagtcgg
      121 ctcggctgtt cccgggtgcc cgcgagggcc tggacgcctt gcgtgcggcc ggtttgccct
      181 tggcttgcgt taccaacaaa ccagccgaat tcaccatacc gctgcttgag cgcctcggca
      241 ttgccgagta tttctcggcc gtcgtcgccg gcgacacgtt gccctacaag aagccgcgcc
      301 ccgaaccgat ccgccacgcc tgcgcgcaga tcggcgtgtc cgccaccgaa aacgtccata
      361 tcggcgactc gctgcacgat tgcgaggcgg cccatgcggc gggctgctgc tacttccacg
      421 tgccctacgg ctatgcagat ccggcgcagc cggtgccggt tgccattggc aaatgcgatg
      481 cgctagtatc cgacctcgtc gacgccgcat cgcgcgtcgg cgccatcaat cagggccaac
      541 actgaccgac tgctcgccgc acacgccatc gtgaaaccgg aaaacgttca atcgccaatc
      601 gccttcctgc tggcccgctg cctcgccggc tggtggcgtt cgcgcccgat ctgaacgcag
      661 gccgcgcccc gggccgatat ggggctaccc gaacgctttc acgaacacct ggctgccgtg
      721 ccatcgcgca acgcgacagc cctcgccgga tgagtacgtc atgaccgaaa ccgaattcaa
      781 tgccctcgcg gcgcagggat acaaccgcat cccggtcacg ctcgagacct tcgccgacct
      841 cgatacgccg ctgtcgatct acctcaagct ggccggcggc ccttattcct acctgctcga
      901 atcggtgcag ggcggcgagc gtttcggtcg ctactccttc atcggcctat cgagcccgac
      961 gcggatcgcc gtctacggcc acgacgtcat ggtgcttacc gggcaccgga tcagcgagcg
     1021 cgagcaggat acgaatccgc tcgacttcat cggcaagtac ctgtcgcgat ttcgcgccgc
     1081 gccgatggcc ggcctgccgc gcttctgcgg tggactggtc ggctatttcg gctacgatac
     1141 cgtgcgctac atcgagacgc gtctcacgcg caccggcaag aacggcgccg gcgctggcga
     1201 cctcggcacg cccgatatcc tgctgctgct ttccgaagag attgcggttg tcgataacct
     1261 ctcgggcaag ctgacgctgg tcgtctatgc cgagcctggg gtgcccggcg cctacggcaa
     1321 agcccgtcag cgcctgcgcg agctgctcgg tcgcctgcgc gagccggcga agattcccga
     1381 cgagacgccg cacaaggcgc cggccgcggc gtcggtcttc ggcgaagcag cattcaagaa
     1441 ggccgtgctc aaggccaagc agtacatcac cgacggcgac atcatgcagg tcgtcctctc
     1501 gcagcgcatg agcaaggcct acggggccac tccgctggcg ctctaccgcg cgctgcgcac
     1561 gctcaacccc tctccctaca tgttctattt cgacttcgag gatttccagg tcgtcggcgc
     1621 ttcccccgag attctcgtcc gtctcgaagg cgacgcggtg acggtgcgcc cgatcgccgg
     1681 cacccgccgc cgtggtgcca gtgccgagga ggatcaggag ctcgccgacg agctgctcgg
     1741 cgacgaaaag gagcgtgccg agcacgtcca gctgctcgac ctcgggcgca acgacatcgg
     1801 ccgtgtcgcg cagaccggat cggtccgcgt cacagaaaac atgctggtcg agcgctattc
     1861 gcacgttatg cacatcgtct cgaacgtcga gggacggctg ctgccgggcc ttgatgcgct
     1921 ggctgtgctc aaggcgacct tcccggccgg caccgtttcc ggtgcgccca aggtgcgggc
     1981 gatggaaatc atcgacgaac tcgaaccggt caagcgcggc gtctacgccg gcgccgttgg
     2041 ctaccttggc ttccacggcg acatggacgt tgccatcgcc attcgcaccg gtgtggtcaa
     2101 ggacggcgag ttgcacgtgc aggctggtgc cggcatcgtc gccgactccg acccggccgc
     2161 cgagtggcag gaaacgcaga gcaaggcccg cgccctgctg cgcgccgccg agatggccga
     2221 agacggcctc gatacgagcc tggaatgagg ccagccatga tgcttatgcg cgcttcggtc
     2281 gccgctcgtc caatgagacg ttgcgatacg ttcgagattg ttttccggga gtcatcgcca
     2341 tgctgctgat gatcgacaac tacgactcct tcacctacaa cctcgtccag tacttcggcg
     2401 aactgggcca gcgggttgag gtctatcgca atgatgcaat cggtgtcgcc gaaattgcgc
     2461 gtctcaagcc tgcttaccta gtggtttcgc cggggccgtg tgcgccgaag caggcgggta
     2521 tatcgctggc ggcgatccgc gagtacgcgg gaaagattcc gctgcttggg gtctgcctcg
     2581 gtcatcaggc aatcggcgaa gctttcggcg gcaagatcgt gcacgccagg cggctcatgc
     2641 acggcaaggt cagcccggtt acgcatacgg gcaagggggt ctttgccggc ctaccgaacc
     2701 cgctgacctg tgcgcgctac cattcgctgg caatcgaacg tgccagcctg ccggactgcc
     2761 ttgaaatcac g
//
LOCUS       DATDAA010000442         4619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_487253,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000442 DATDAA010000000
VERSION     DATDAA010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487253"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VIS73_13170"
     CDS             complement(<1..602)
                     /locus_tag="VIS73_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5764152.1"
                     /translation="MLLIASETIRSNRTLNDVVREMSFTLSFFASPATFTELMRGQSR
                     RIVLLTEDDVCAETVRTLKGAKDRAPFGVIIAADRRALREVKNAELVDKLASYDNIEW
                     VGKDFDFDHLSASARRCRRRMLRVSRRELESALEHHEIVLRYQPKVERNSGQEWLTRE
                     AEALIRWHHPEHGLIGPLEFLPEAEAFGLMADLSEFVLREA"
     gene            891..1259
                     /locus_tag="VIS73_13175"
     CDS             891..1259
                     /locus_tag="VIS73_13175"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEY5764153.1"
                     /translation="MDLTIPRVLMWQFVVGAALAAILWAVFGTVAGYSALLGSLASVI
                     PNGFLGLRLMAPRRDPGALKKAAWSGEMGKLALTVLLFVLVFTQVKPLSAAALFAGFI
                     AAQLTVFSGFLMRSETDTED"
     gene            1268..2116
                     /gene="atpB"
                     /locus_tag="VIS73_13180"
     CDS             1268..2116
                     /gene="atpB"
                     /locus_tag="VIS73_13180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004773235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY5764154.1"
                     /translation="MAAEGGQGPQSSGDYITHHLQNLQVCKDETGEWVWNQCSGNLMA
                     INVDSMFWSIFLGLTFCYLFWRVATKSSTEKPTKFQAAIEIIVEMVDSSVKDTFHGNS
                     RLIAPLALTVFVWVFLMNLMDLVPVDWIPGLAKLIGIEYMKVVPTTDVNVTFGMSIAV
                     FFLILIYTHMAKGPTALIQDLMLHPIAPSFKGAAILAAPFIMAFNLVLEVVALIAKPI
                     SLSLRLFGNMFAGELIFILIALIGWFQLPLHFGWAVFHVLIVTLQAFIFMMLTIVYLS
                     LASEKH"
     gene            2170..2448
                     /gene="atpE"
                     /locus_tag="VIS73_13185"
     CDS             2170..2448
                     /gene="atpE"
                     /locus_tag="VIS73_13185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY5764155.1"
                     /translation="METVIAFTAIAVALLIGLGALGVGIGMGLLGGRFLEGAARQPEL
                     APMLQTKMFLVVALLDAVAMIGVGIALFFAFANPFVDQLRNALQPVAG"
     gene            2538..3008
                     /locus_tag="VIS73_13190"
     CDS             2538..3008
                     /locus_tag="VIS73_13190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004411.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY5764156.1"
                     /translation="MELNATLLGQTLAMIVFVVFCMKYIWPPILSALEERQKQIEEGL
                     AAADKSQEKLAEATAEADKIVAEARHQATGILDQAHARANEIVAEGKDAGVKERERQL
                     AAAKAEIEQETNKARDELRGQVSAIAIASAEKILNREIDDKAHEDILGKLAQEI"
     gene            3012..3575
                     /locus_tag="VIS73_13195"
     CDS             3012..3575
                     /locus_tag="VIS73_13195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY5764157.1"
                     /translation="MADNNTVARPYARAVFEFAQENGVLTELSESLAAAKALLEDGQV
                     VKFLGTPAISDAERLTFLQGLFAEVVGKDSVFAGGSEHGTNFFRLLLENGRVAALPEI
                     ADRFEALKARVENTVDAVITAAAPLSAAQQQAMAKSLKDKLGRNVNVTTEIDENLIGG
                     AVIRAGDVVIDGSLRARLHGLANALIK"
     gene            3589..>4619
                     /gene="atpA"
                     /locus_tag="VIS73_13200"
     CDS             3589..>4619
                     /gene="atpA"
                     /locus_tag="VIS73_13200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY5764158.1"
                     /translation="MALKASEISTLIKERIENFEASAEARDVGTVIAVTDGIVRIHGL
                     ADARYGEMLEFPGNTFGMALNLEQDSVGAVVLGDYKHISEGATVKTTGRILEVPIGDA
                     LLGRVVDSLGNPIDGKGPIKAEATAPIEKVAPGVIERQSVSQPVQTGLKSIDSMVPIG
                     RGQRELIIGDRQTGKTALAIDTIINQVGTGIKCIYVAIGQKASSIAGVVRKLEEEGAM
                     EHTIVVAAAAADSPAMQYIAPYSGASMGEYFMDKGQDALIIYDDLTKQAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNAAHVEAVSGGKVKGKTGSLTALPIIETQA
                     GDVSAFVPT"
BASE COUNT          921 a         1384 c         1432 g          882 t
ORIGIN      
        1 gcctcacgca gcacgaattc actgaggtcg gccatgagac cgaatgcttc cgcctcaggc
       61 aggaactcga gcggcccgat caggccgtgc tcggggtgat gccagcggat cagggcctcg
      121 gcctcgcggg tcagccactc ctggcccgag ttccgctcca ccttgggctg gtagcgcagc
      181 acgatttcgt ggtgctcgag ggcgctttcg agctcccgcc gcgagacacg caacatgcgc
      241 cgccggcagc gacgggccga tgcgctgagg tgatcgaaat caaaatcctt gccgacccac
      301 tcgatattgt cgtacgaggc cagcttgtcg acgagttcgg cgttcttcac ctcgcgcagg
      361 gcgcggcggt ctgctgcgat gatgacgccg aacggcgccc gatccttggc gcccttgagc
      421 gtacggactg tctccgcaca cacgtcgtcc tcggtcagga gcacgatgcg gcgggactgc
      481 ccgcgcatga gctccgtgaa cgttgccgga ctggcaaaga aacttaaggt aaagctcatc
      541 tcccgcacaa cgtcattgag ggtgcggttg gagcgaatgg tttcacttgc aatcaaaagc
      601 atggcggccg gaattatggt gactcacccc ctatttatac cgggcaaaag cggactcgaa
      661 tgtgcgccgt gtcgcattgg gagaaatttg ccgaaacccc ataaattcgc gggaaaaagt
      721 ggcatgaagc gatctggaaa tgagcggcgt ttcgtgttgc cacacacagg tagcgcctct
      781 ataatgcgca cgcaattcgc aggcccgcct ggaatgaaca cacctcaagc gccacacccg
      841 caggcttaag gcttcggaag tggcgttttt agtgccggag gactggagaa atggatctca
      901 ctatcccccg cgtgctgatg tggcaattcg tggtcggggc ggccctggcc gcaatcctct
      961 gggccgtttt cgggacggtg gctggttact cggcactgct gggcagcctg gccagcgtta
     1021 tcccgaacgg atttctgggc ttgcggctca tggcaccacg ccgtgacccg ggtgcgctca
     1081 agaaggcggc ctggagcggc gaaatgggaa agcttgcgtt gaccgtgctc ttgttcgtgc
     1141 tcgtgttcac gcaggtcaag ccgctctcgg cagcggcact gtttgcagga ttcatcgctg
     1201 cgcagctgac ggtcttttcc ggcttcctga tgcgcagcga gactgatacg gaagactgag
     1261 taaagacatg gcagctgaag gcggacaagg gcctcaatcg tcaggcgatt acatcaccca
     1321 tcacctgcag aacctgcagg tctgcaagga cgagaccggc gagtgggtct ggaatcaatg
     1381 cagtggcaac ctgatggcga tcaacgtcga ctcgatgttc tggtcgatct tcctcggcct
     1441 gaccttctgc tacctgttct ggcgcgtggc gacgaaatcg tcgaccgaaa agccaacgaa
     1501 atttcaggcc gccatcgaga tcatcgtcga aatggtcgac agttcggtca aagacacctt
     1561 ccacggcaat agccgcctga tcgcaccact ggccctgacc gtgttcgtct gggtgttcct
     1621 gatgaacctg atggacctgg tgccggttga ctggatcccg gggctggcga agctgattgg
     1681 catcgagtac atgaaggtgg taccgacgac ggacgttaac gtcacgttcg gcatgtcgat
     1741 cgcggtcttc ttcctgatcc tgatctatac ccacatggcc aaggggccga cggcactgat
     1801 ccaggacctg atgctgcacc cgatcgcgcc gtcgttcaaa ggcgcggcaa tcctggctgc
     1861 gcccttcatc atggcgttca acctggttct cgaggttgtg gcgctgatcg ccaagccgat
     1921 ctcactgagc ctccggctgt tcggcaacat gtttgccggg gaactgatct tcatcctgat
     1981 tgcgttgatc ggctggttcc agctgccgct gcacttcggc tgggcggtat tccacgtcct
     2041 gatcgttacg cttcaggcgt ttattttcat gatgctgacc atagtttatt tgagcctggc
     2101 atcagagaag cactgattcg atttgcttta aaaaccaacg tttggattga cactccctag
     2161 gagaaactga tggaaactgt tatcgcgttc acggctatcg ctgtcgcact cctgatcggc
     2221 cttggcgccc tcggtgtagg tatcggcatg ggcctcctcg gcggccgctt cctggaaggc
     2281 gccgcccgcc agccggagct ggcaccgatg ctgcagacga agatgttcct cgtcgtcgcc
     2341 ctgctcgacg ccgtagcgat gatcggtgtc ggtatcgcgc tctttttcgc gttcgccaac
     2401 ccgttcgttg accagctgcg caacgccctg cagccggtcg ctggataaac gccggtaggg
     2461 ggttttaacc ccgctagcgg gttcgttcgc ggcgcacgcc gctcctgcgg gccctgctac
     2521 cggtattgga gaaatccgtg gaattaaatg caactctctt aggccagacc ctcgcgatga
     2581 tcgtcttcgt cgtgttctgc atgaagtaca tctggccgcc gatcctcagt gcgctcgaag
     2641 aacgccagaa gcagatcgag gaaggtcttg ctgcggccga caagagccag gagaagctcg
     2701 ccgaggcgac ggccgaggcc gacaagatcg tcgcggaagc tcgccaccag gccaccggca
     2761 ttctcgacca ggcacacgcc cgcgccaacg aaatcgtggc cgaaggcaag gacgccgggg
     2821 tgaaggaacg cgagcgccag ctcgccgccg ccaaggccga gatcgagcag gaaaccaaca
     2881 aggcgcgcga cgaactgcgc ggccaggtat cggctatcgc gatcgcttct gccgagaaaa
     2941 tcctgaaccg cgagatcgac gacaaggcgc acgaggacat cctgggcaag cttgcgcagg
     3001 agatttgagc cgtggctgac aacaacaccg tcgctcgccc gtatgcccgg gccgtcttcg
     3061 aatttgcgca ggaaaacggc gtgctgaccg agctgtcgga atccctggcg gccgcgaagg
     3121 ccctgctcga agatggacag gtcgtcaagt tcctggggac gcccgcgata tcggatgccg
     3181 agcggctgac gttcctgcag ggactgtttg cagaagtcgt gggcaaggac tcggtctttg
     3241 ccggcggaag cgagcacggc acgaacttct tcaggctgtt gctcgagaac ggccgcgtcg
     3301 ccgcgttgcc ggagatcgcg gaccggttcg aggccctcaa ggccagggtc gagaacactg
     3361 tcgacgcagt catcacggcg gccgcaccgc tcagcgcagc acagcagcag gccatggcga
     3421 agtcgctgaa ggacaagctc ggacggaacg taaacgtcac cacggaaatt gacgaaaatc
     3481 tcatcggtgg cgccgtgatt cgcgccggcg atgtcgtaat tgatggatca ctgcgtgcca
     3541 ggctccacgg cctcgcgaac gcgctgatca agtaaagagg aaaaggaaat ggcactcaaa
     3601 gcttctgaaa tcagcacgct gattaaagaa cgcatcgaga acttcgaggc atccgcggaa
     3661 gcgcgtgacg tcggcaccgt tatcgcggtt accgacggca ttgttcgcat ccacggcctg
     3721 gctgacgctc gctacggcga gatgctggag ttcccgggca acaccttcgg tatggcactc
     3781 aacctcgagc aggactcggt gggtgcggtt gtcctgggtg actacaagca catctcggaa
     3841 ggcgcgaccg tcaagacaac gggccggatt ctcgaggtgc ccattggtga cgccctgctg
     3901 ggccgcgtcg tcgactcgct cggcaacccg atcgatggca agggcccgat caaggccgag
     3961 gccacggcgc cgatcgagaa ggttgcgccg ggcgtcatcg agcgccaatc ggtttcgcag
     4021 ccggtccaga cgggacttaa gtcgattgac tcgatggtgc cgatcggccg tggccagcgc
     4081 gagctgatca tcggtgaccg ccagacgggc aagacggctc tcgcgatcga cacgatcatc
     4141 aaccaggtcg gcacgggcat caaatgcatc tacgttgcta tcggccagaa ggcctcctcg
     4201 atcgcgggcg tcgtaagaaa gctcgaggaa gagggcgcga tggaacatac gatcgtcgtt
     4261 gctgccgctg ccgccgacag ccccgccatg cagtacatcg caccgtactc cggtgcgtcg
     4321 atgggtgagt acttcatgga caagggtcaa gacgcactca ttatttacga cgatctcacg
     4381 aagcaggcct gggcctatcg ccaggtgtcg ctgctcctca ggcgtccgcc gggccgtgaa
     4441 gcctatccgg gcgacgtttt ctacctgcac tcgcgcctcc tggagcgagc tgcccgcgtc
     4501 aacgcggcac acgtcgaggc agtgtcgggt ggcaaggtga agggcaagac cggctcgctc
     4561 acggcgctgc cgatcatcga aacgcaggcc ggtgacgtat ccgcattcgt gccaacgaa
//
LOCUS       DATDAA010000443         3460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_276498,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000443 DATDAA010000000
VERSION     DATDAA010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276498"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /gene="mnmC"
                     /locus_tag="VIS73_13205"
     CDS             <1..609
                     /gene="mnmC"
                     /locus_tag="VIS73_13205"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR03197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEY5764159.1"
                     /translation="GADGIDLGRYEQVVLAGATDICSLAVSQHLPLFRFRGPVTHLPD
                     EAIPGLKAVVCREGYLTPALDGQHCVGASFHRGGEPAIRAEDHLANLDRLERMIPGTA
                     ARWREQPLEGRVGFRPVSPDKVPIVGRLQTADAQAQGRDLSAVPRLPGLHVATGYGAR
                     GLVWAPLMAEVLACEIAGEALPIESDLAGAIDPARFVARARR"
     gene            629..2239
                     /locus_tag="VIS73_13210"
     CDS             629..2239
                     /locus_tag="VIS73_13210"
                     /inference="COORDINATES: protein motif:HMM:NF028537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HEY5764160.1"
                     /translation="MRLPPASPIFVALLVALYLTVALNVAFFSGVVVAFSDTLAGPFI
                     VSTALVVWAVHALLLLPFSGRLLIRPVAAVLILITAPAAYFAFAYNVHFDATMLRNVA
                     QTNPAEAFELLSLRFIGFVIALGLLPAALVLRIPLREGGIQRPLLGRLLIGVALLVLA
                     IACIVGFSSRYATLFREHKALRYQVLPAALIHAGFRYAAETLRRDAAMTPVATDARVA
                     ESDRDRELLILVVGETARGDHFSLSGYARQTNPLLAGEQVISFRDVEACGTSTAVSVP
                     CMFSIYDASDYSDAKGRETETLLDALTHAGVHVLWRDNNSSSHSVAGRVPYEDFKDPA
                     RNPVCDEECRDEGMLAGLQQYIDGHPAGDIVIVLHQMGNHGPAYYKRYPPRFERFTPV
                     CRTVQLAECSRDEIVNAYDNAILYTDYFLSQVIALLKQNTGKFEAAMLYLSDHGESLG
                     ENGLWLHGMPKAIAPGEQTRAASIFWFGDSFEPADAAAVRQRQGQVISHHNLFHTVLG
                     MLEIETHDYDPRLDLRHPQAGAALSRPD"
     gene            2302..>3460
                     /locus_tag="VIS73_13215"
     CDS             2302..>3460
                     /locus_tag="VIS73_13215"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY5764161.1"
                     /translation="MNDFPRPPHAATLAARLVANRLTRRDVLWLFGAGAGAAAASALG
                     GCATSPVSGKRILVGMSEAQERAIDQEVAPHQFSQDLGAVQENGVNRYVAETGARLHV
                     LTHRPGMPYSYRVLNANYVNAYTFPGGAMGVTRGIMTELDNEAELAALLGHELGHVNA
                     RHAAQRQGQAMVAQAAVAGLGVAGADSPWGGVLAIGGQVGASALLSSYSRDNEREADA
                     LGQDYMVQAGYPASGMTELHGLLVAQEKSQPSLLQTMFSSHPMSGERMETARQLATTR
                     YAASLKVNPQRERYMDNTAGLRRLKPAIDACKNGEAAMAGKKLPEAERQFGLALKAAP
                     RDYAANVLMARCLAAQDKSTEAMRYVEAARTIYPQEAQAHKLAGVLQLQARD"
BASE COUNT          548 a         1238 c         1106 g          568 t
ORIGIN      
        1 ggcgccgacg gcattgatct cggccgctac gagcaggtcg tgctcgccgg cgccaccgac
       61 atctgctcgc tcgccgtctc gcaacacctg ccgctgtttc gcttccgcgg cccagtcacg
      121 cacctgccgg acgaggcgat cccggggctc aaggccgtcg tctgccgcga gggttacctg
      181 accccggcgc tcgacggtca gcactgcgtt ggcgcctcgt tccatcgtgg cggcgagcct
      241 gcgattcgcg ccgaggatca tctggccaac ctcgaccggc tcgaacgcat gatcccgggc
      301 acggccgccc gctggcgcga gcagccgctc gagggtcgcg tcggcttccg tccggtttcg
      361 cccgacaagg taccgatcgt cggccgcctg cagaccgccg acgcccaggc gcagggccgc
      421 gacctgtcgg cggtaccgcg gctgccaggg ctccacgtcg ccaccggcta tggcgcgcgc
      481 ggcctcgtct gggcgccgct gatggccgag gtgctggcct gcgagattgc cggcgaagcg
      541 ttgccgatcg aatcggacct ggccggcgca atcgacccgg cgcgcttcgt cgcccgggcc
      601 cggcgctgat cgtataaatt tccacgcgat gcgtctgcct cccgcctcgc cgatcttcgt
      661 cgcgctgctc gtcgcgctct acctcacggt ggcactcaac gtggcgttct tttccggcgt
      721 ggtcgtcgcc ttcagcgaca cgctcgccgg gccgttcatc gtctcgaccg cgctggtggt
      781 ctgggcggtt cacgcgctgc tgctgctgcc cttcagcggg cggctgctga tcaggccggt
      841 tgcggctgtg ctgattctga tcacggcgcc ggctgcgtat ttcgccttcg cctacaacgt
      901 gcatttcgac gcgacgatgc tgcgcaatgt cgcgcagacc aatccggcgg aggcattcga
      961 gctgctcagc cttcgcttca tcggcttcgt cattgcactc gggctgctgc cggcggccct
     1021 ggtgctgcgc attccgttgc gcgaaggcgg aattcagcga ccgctgctgg gccgcttgct
     1081 gatcggcgtc gcgctgctgg tgctggcgat cgcctgcatc gtcggtttca gcagccgtta
     1141 tgcaacgctg ttccgcgagc acaaggcgct gcgctaccag gtactgccgg cggcgctgat
     1201 ccatgccggc ttccgctatg ccgccgaaac cctgcgccgc gacgccgcga tgaccccggt
     1261 ggcgaccgac gcgcgcgttg ccgagtccga ccgcgaccgc gaactgctga ttctcgtcgt
     1321 cggcgaaacc gcccgcggcg accacttctc gcttagcggc tacgcgcggc agaccaatcc
     1381 gctcctggcg ggcgaacagg tgatttcctt ccgtgacgtc gaagcctgcg gcacctcgac
     1441 cgcggtctca gtcccttgca tgttctcgat ctacgatgca tcggactatt cggacgccaa
     1501 aggccgggaa accgaaaccc tgctcgacgc cctgacccac gccggtgtcc acgtgctctg
     1561 gcgtgacaac aactccagct cgcactcggt cgccggccgc gttccctacg aggatttcaa
     1621 ggacccggca cgcaacccgg tctgcgacga ggaatgccgc gacgaaggca tgctcgccgg
     1681 cttgcagcaa tacatcgacg gccatcccgc cggcgacatc gtcatcgtcc tgcaccaaat
     1741 gggcaaccac ggccccgcct actacaagcg ctatccgccg cgtttcgaac gcttcacccc
     1801 ggtctgccga accgtgcaac tggccgagtg ctcgcgcgat gaaatcgtca acgcctacga
     1861 caacgcaatc ctttacaccg actacttcct gtcgcaggtc atcgccctgc tgaagcagaa
     1921 cacaggtaag ttcgaggcgg ccatgctcta cctcagcgac cacggcgaat cgctcggcga
     1981 aaacggcctc tggctgcacg gcatgccgaa ggccatcgcg cccggcgagc aaacccgcgc
     2041 cgcgtcgatc ttctggttcg gcgacagctt cgagccggcc gacgctgccg cggtccgcca
     2101 gcgacagggg caggtaatca gccaccacaa cctcttccat accgtgctcg ggatgctgga
     2161 gatcgagacg cacgattacg atccacgcct ggacctccgg cacccgcaag ccggggcagc
     2221 cttgtccagg cccgattagc tgcctgctaa cggcgcctga atcggcataa tctgcttatc
     2281 gcctcaccgc cggagcctgc gatgaacgac ttcccccgac cgccccacgc tgccacactc
     2341 gccgcacggc tggtcgccaa ccgcctcacc cgccgcgatg tgctctggct gtttggtgcg
     2401 ggcgccggtg cggcggccgc atcggcgctt ggcggttgcg ccacctcgcc ggtaagcggc
     2461 aagcgaatcc tggtcggcat gagcgaggcg caggagcgcg ccatcgacca ggaggttgcg
     2521 ccgcaccagt tctcgcagga cctcggtgcc gtgcaggaga acggcgtaaa ccgctacgtc
     2581 gccgagaccg gcgcacgcct gcacgtactg acgcaccggc cgggcatgcc ctactcgtac
     2641 cgcgtgctca atgccaacta cgtcaatgcc tataccttcc ccggcggcgc gatgggcgtt
     2701 acccgcggga tcatgaccga actcgacaac gaggccgaac tcgccgcgct gctcggccac
     2761 gaactgggcc acgtcaacgc gcgccacgcc gcccagcggc agggacaggc gatggtcgcg
     2821 caggcggcgg tcgccggcct gggcgtcgcc ggcgccgatt cgccgtgggg tggcgtactg
     2881 gcgatcggcg gtcaggtcgg cgccagcgcg ctcttgtcgt cctactcgcg cgacaacgag
     2941 cgcgaggccg atgcgcttgg gcaggattac atggtccagg ccggctatcc cgcgagcggc
     3001 atgaccgaac tgcacggcct gctggtggcg caggagaaat cgcaaccctc gctgctgcag
     3061 acgatgtttt cctcgcaccc gatgagcggc gagcgcatgg aaacggcgcg gcagcttgca
     3121 acgacgcgat acgccgcgag cctgaaggtg aatccgcaac gcgagcgcta catggacaac
     3181 accgccggcc tgcgccgcct gaagccggcg atagatgcgt gcaaaaacgg cgaggcggcg
     3241 atggcgggca agaagctgcc ggaagccgaa cgccagttcg gcctggccct aaaggcggcg
     3301 ccgcgggact acgcggcaaa cgtgctgatg gcgcgctgcc tggcggcgca ggacaagtcg
     3361 accgaggcga tgcgctatgt cgaggcggcg cgcacgatct accctcagga ggcacaggcg
     3421 cacaagctgg ctggcgttct gcagttgcag gctcgcgatc
//
LOCUS       DATDAA010000444         2208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_242453,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000444 DATDAA010000000
VERSION     DATDAA010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242453"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VIS73_13220"
     CDS             complement(<1..462)
                     /locus_tag="VIS73_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764162.1"
                     /translation="MHSLMPADFPHALLDHSPDAVAGDRAFRVDAGFDRTGRGLQLRY
                     RLRGPIAELRLDHAPGSLPSARLWAHSCFEAFVAADAARYREFNFAPNGQWAAFDFVD
                     YRQPAEPTATPSAAPLLESWRDGDDFFLVAELPDDLIPASDGERALGLSAVL"
     gene            725..1132
                     /locus_tag="VIS73_13225"
     CDS             725..1132
                     /locus_tag="VIS73_13225"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HEY5764163.1"
                     /translation="MGKIEWGPEYETGVREIDSQHRTLLGIYNDLSDALELDTAGQAA
                     ATTLDRLLHYTDFHFKTEERMMVEHGYPMSSAHTAEHYVLIERSKLLQHRLQGGDQKV
                     AVDTLDFLKRWLINHIQKVDRDLAEYLQSRGVN"
     gene            complement(1145..1501)
                     /locus_tag="VIS73_13230"
     CDS             complement(1145..1501)
                     /locus_tag="VIS73_13230"
                     /inference="COORDINATES: protein motif:HMM:NF020469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HEY5764164.1"
                     /translation="MLVSFESSTDGQLQMFADVAAPLLRYLRKTPAPRGVITAEELPE
                     LLAALQRLRDGSDRAAPLDGDAHAAADLDANEIPVSLRQRVAPLIELMQRTMGNEGYL
                     MWRAPPDFGSENSGRR"
     gene            1574..>2208
                     /locus_tag="VIS73_13235"
     CDS             1574..>2208
                     /locus_tag="VIS73_13235"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5764165.1"
                     /translation="MSHLDDFTAGDSWNDLIGVPGDEVERLAVQEVTRRQRNDDPVSV
                     LDADCGHGTLAVRLAQAGASVTALIDCAKGPGKTARGLIAKDLPIAFVACRAIDLTAP
                     PAGGPFDLIVLRRSLPFLRYAEATALLTRLNGWLKIGGKIIVSTFGVHSHFGDAYPDV
                     EKPVQKRFAPLPDELARQYGIAGPICLYSERDLFILVFESGIGVLRSFTTT"
BASE COUNT          413 a          726 c          714 g          355 t
ORIGIN      
        1 aaggacggcc gacaggccga gcgcccgttc gccgtcggac gccgggatta ggtcgtcggg
       61 cagttcggcg acgaggaaga aatcatcgcc gtcgcgccac gattcgagca gcggagcggc
      121 agaaggggtc gccgtcggct ccgctggttg tcgataatct acgaaatcga atgccgccca
      181 ctggccattg ggtgcgaagt tgaattcgcg atagcgcgcc gcatcggcgg cgacaaaggc
      241 ctcgaagcag ctgtgcgccc agagccgcgc gctcggcagc gaacccggcg catggtcgag
      301 gcggagctcg gctatcggtc cgcgcaggcg gtaacgcagc tgcagtccgc gcccggtccg
      361 atcgaagccg gcatcgacgc gaaacgctcg gtcaccggcg acggcgtccg gcgaatggtc
      421 gaggagggcg tgcgggaaat cggcgggcat gaggctgtgc attctagggc acggccgatt
      481 cgacgcgaaa cacaatgact aggcagttgt catcggcgta ggtcggcgcg ccgaaatgcg
      541 ccgctgtgac agagtgtgca gcgcgcgaca ttatcgccga atcgccctaa ttacgggctt
      601 gtcggcagga tggccgcagg tataatcccc catcgccaag gccgcgcaac cgaaatgtgc
      661 cggacgatac cagccagtga cgcgcaatcc gccgcgggtc gcgcgggcaa caacaggaga
      721 cgtggtgggg aaaatcgagt ggggcccgga atacgagacc ggcgtccgtg aaatcgacag
      781 ccagcatcgc acgctgctcg gaatctacaa cgatctcagc gacgcgctcg aactggacac
      841 cgccggtcaa gctgccgcca cgaccctcga ccgactgctg cactacaccg atttccactt
      901 caagaccgaa gaacggatga tggtcgagca cggctatccg atgtcgagcg cgcatacggc
      961 cgaacactac gtgctgatcg agcgatccaa gctgctgcag caccgactgc aaggcggaga
     1021 ccagaaggtg gcggttgata cgctcgactt cctcaagcgc tggctgatca accacatcca
     1081 gaaggtcgac cgcgacctcg ccgagtatct ccagtctcgc ggcgtgaact gaggccgctg
     1141 cgcctcagcg gcgccctgag ttctcgctgc cgaaatcggg tggcgcccgc cacatcaggt
     1201 atccctcgtt gcccatcgtc cgctgcatca actcgatcag aggcgcaaca cgctgccgca
     1261 gcgataccgg gatttcgttg gcatcgaggt cggcggccgc gtgggcatcg ccatcgagcg
     1321 gtgcagcccg gtcgctgcca tcgcgcaggc gttgcagggc cgcgagcaac tcaggcagtt
     1381 cctcggccgt tatgacgccg cgcggcgcgg gtgtcttgcg caggtagcgg agcaggggtg
     1441 cggcgacgtc ggcgaacatc tgcaactggc cgtcggtcga ggattcgaag ctcacaagca
     1501 tgatttcaat ccgtgcgaga atgcggccac cttaccgaga acggccgcca gaagccagcc
     1561 atccgacacc gcgatgagtc atttagacga tttcactgcc ggcgacagct ggaacgacct
     1621 gatcggcgtt cccggcgatg aggttgagcg cctcgcggtc caggaagtca cgcggcgcca
     1681 gcgcaacgac gatccggttt cggtcctcga tgctgactgc ggacacggca cgctggctgt
     1741 tcgcctggcg caggccggcg cctcggtcac cgcgctgatc gactgtgcga agggcccagg
     1801 caagacggcg cgcggactca tcgccaagga cctgccgatc gccttcgtcg cgtgtcgcgc
     1861 gatcgatctc acggcgccgc cggcgggtgg cccctttgac ctgatcgtcc tccgccgcag
     1921 cctgcccttc cttcgctacg ccgaggcaac ggcgctgctc acccgcctca acggctggct
     1981 gaagatcggc ggcaagatca tcgtctccac cttcggcgtt cattcgcact tcggcgacgc
     2041 ctaccccgac gtcgagaaac ccgtgcagaa gcgcttcgcg ccgctgcccg acgaactcgc
     2101 ccggcagtac gggatcgccg ggccgatctg cctttattcc gagcgcgacc tcttcatcct
     2161 cgtcttcgag tcgggaattg gcgtgttgcg ctcgtttacc acgaccca
//
LOCUS       DATDAA010000445        31286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_66853,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000445 DATDAA010000000
VERSION     DATDAA010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 31286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31286
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66853"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..2446
                     /locus_tag="VIS73_13240"
     CDS             194..2446
                     /locus_tag="VIS73_13240"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5764166.1"
                     /translation="MLALTAITAIALVRLWLAGTGAHDAERADTEAAATRHATTIAAV
                     TAETLRSRLESLDHTLRQMAIAYAEHQQIPLEDLRITSLSQPPGLALNFVRIDARGFV
                     RDDEYQTERAGQPQRYLGDLPLFRYHASNPDDALRVDPPAKSRLVDRWTIPVSRVLRD
                     DEGRFAGVVLLELSPEQLSADFRALGLDSNDVIGIIHADGSYLARSYRLDEALGNKVR
                     GDHAYLFPGAALSGSFRGQGGVDGVERIWAYQRLPGFELSVVVGLDLTGPSHSMLARH
                     ESERDNMILATAMAVALLGALLALLWRLDASLRALQASEGRLKRALEGASEVAWEWRA
                     DSDRVTLLGNCRLFLGIAEPSIELELAAWRGRVHPSDLPSLTDAWREHLAGQADEFEA
                     EYRVLGGENSYRWVLDRGLTEHRDAQGRPSRIAGVLIDIDPIKRAQLTITRLSARYQQ
                     LFASANEGIYVVNTQGEIEMMNRAAQTLTGWREEEVLGKQAHALFHEALAGHPDHSWP
                     TCPLNMAMKTGDVRRGERMLYWRQDGSAMPVELSIGPLLEGDECDGAIVVFSDITERL
                     ATERKLELLATTDELTGLANRRRFVEIAERDLRRAAREGTPATLIMMDLDHFKTVNDR
                     FGHAMGDRVLKGVTDACAAQLRAVDVFGRLGGEEFAVLLANIEPERALEVAERMRVAA
                     AEREIRDGQRSVAVTVSLGLAHAGFGETLDSLLARADAALYGAKESGRDRIVVADPAG
                     AETAQADASA"
     gene            2554..3414
                     /locus_tag="VIS73_13245"
     CDS             2554..3414
                     /locus_tag="VIS73_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764167.1"
                     /translation="MESLARALLLSVALAFAGAAAAQGGGDSDAQRRLLEQKLRLVEM
                     LLKSPAAQAREGEAAASVAEGREALARARTALSEARLDEAATLADQALKSVSAASRRG
                     ASAPLSESAQRKSLDDLGSQLASYRSSIVELGQDPKLGDAARDLLTRIDSARDESNRL
                     AADGQLGDANRTLAAAYRLAVEEISRLRQGQEVVMSLKFDSPADEYAYDQRRFASNEI
                     LVEQMVAQGKADGGRQMLVDKFSADARRIRDEAEGLAAAGDHPGAVVLMEKAVGQLNR
                     ALQTMGVPVF"
     gene            3424..4230
                     /locus_tag="VIS73_13250"
     CDS             3424..4230
                     /locus_tag="VIS73_13250"
                     /inference="COORDINATES: protein motif:HMM:NF025055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HEY5764168.1"
                     /translation="MISLTRRSGVFSRATLALLAILAAGPLFAAAADDALATINIAGE
                     QRMLSQRIVKAYLQLGLNVQPLAAKTQLDSAIRRFEANQQLLLPAIAGDPDSRRAGEL
                     LTTAWEQMRPLTSGAPTLENAQRLSAYGETVLNAAEMLVNVYADRSGVDEHRLLRLMA
                     RQRMLSQRIAKTYLLRSWGDQSAQLRYQSEDAIIAFEGVLTMFQSRRDEGPMLRQELD
                     ELALQWEWMKAALESDGALSYRLIVAESADAILASADRLAQQYQRLLAAR"
     gene            4244..5665
                     /locus_tag="VIS73_13255"
     CDS             4244..5665
                     /locus_tag="VIS73_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEY5764169.1"
                     /translation="MTMQRRDFLKGLATGASLAAGGLPRLAQAAPMVTSLNAGAVEQI
                     LTPGGGVSNLWGYNGTVPGPLLRFRKGETVRIPVRNGLPADTTVHWHGLRVPNAMDGV
                     PYVTQNPIKPGETFQYEFTIPDSGSFWYHPHQSSYEQVPRGLYGPLIVDEEKPIEIDQ
                     EAIWVLSDAKLEANGRQVEDFGRVLDLANEGRLGNVVLINGLAAGAERSLKVRSGDRV
                     RLRLINAAASRIFKLAFAGHVVQAIAFDGQAVTPHPLEEIYIGPGMRIDLIIDCMHAP
                     GSSFTIANRDRRSLGDLARIVYTPEKPRRSKPMKAPIALQPNRFPEPDLKKAIDHYVV
                     FEGGMRGAPVIGQVDGKPAKIQDIMEKYGLAWTMNFSAQHEHALMHEPLFYLKKGDHV
                     LLRMINETDFVHPMHLHGHFFRVVAIDGVKTVHQEWRDTVMMGPRQTVDVAFVADNVG
                     EWMYHCHILDHAAGGMMGTIAVE"
     gene            complement(5680..7407)
                     /locus_tag="VIS73_13260"
     CDS             complement(5680..7407)
                     /locus_tag="VIS73_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5764170.1"
                     /translation="MPKNAAAAEHLVRADLTGLRVLIVDRHSNARDSLRLMLASLSIT
                     KVHGASSSVEALRLVRASPFDVVFSDYLLGDGRDGQQLLEELRVKHLIPLSTVFFIVT
                     SERGYHNVVSVAELTPDDYLVKPFTAEQLQSRLAHALYAKKVLSPILRELDAAAYGRA
                     LAACEKLLAGDNDFYLETLRHKGEILNLLGRHDEAIALLREVLATRPLPWAKMGLAMA
                     LRATGQIDEAETLARELLRDHPHFLAAHDFLAALLEERGNLDDARSVLASAAEISPHN
                     TGRQRIVGEVAARSGHHELAEKAFQAVLTRTRGSSLSTADDYANLSRALLDGGKASAA
                     RSVAQEMRRDRRWDPTTEVAASVVESLCHAEEGEPDKARAALDRALSAAEGLSEKDKA
                     GFALPEKIAVDLAHACLASGEAEKAGQVLRQVAAEHQENTALIDRMRDIFDRTENTDA
                     GLAILDEVSREIIDINNRGVLTARQGDLAESVRLLCAAADRIPNLQFLVNATSAIFTL
                     LDRQGWQDPLAERALGYLLRAQRRDPRHSRVIAAGELFQAVAQKYGINAAALRQQVAD
                     SLRGSGTAR"
     gene            7590..9488
                     /gene="mnmG"
                     /locus_tag="VIS73_13265"
     CDS             7590..9488
                     /gene="mnmG"
                     /locus_tag="VIS73_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEY5764171.1"
                     /translation="MQYPNRFDVIVVGGGHAGTEAALASARSGARTLLLTHNLETLGA
                     MSCNPSIGGIGKGHLVKEVDALGGAMAAATDEAGIQFRILNASKGPAVRATRAQADRT
                     LYRQAIRSRLENQPNLWLFAQAVDDLIVDGDRVCGAVTQAGVRFAADAVVLTAGTFLN
                     GLIHIGLQNHPGGRAGDPPAVSLAARLKELKLPQGRLKTGTPPRLDGKSIDFSLMQEQ
                     HSDAPLPVFSFLGSADQHPQQLPCWITDTNERTHEIIRGGLDRSPMYTGVIEGVGPRY
                     CPSIEDKIHRFADKASHHVFLEPEGLDTHEVYPNGISTSLPFDVQLEMVRSIRGLENC
                     HILRPGYAIEYDYYDPRGLRDSLETKAIGGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RRTRGEAPWCPRRDEAYLGVLVDDLITRGVSEPYRMFTSRAEFRLSLREDNADLRLTE
                     IGRRLGLVDDQRWAAFSRKREAIERESARLRGTWLTPARLGEAEAARVLGKPIEREYT
                     LLDLLRRPGIAYASLMTLPGAGPGEADPQVIEQLEIAARYQGYIVRQQDEIARVAASE
                     TTAIPDDLDYAQVRGLSKEVQQRLEQQRPRTIGQAGRLQGVTPAAISLLLIHLRRVSA
                     TRNIDRAA"
     gene            9485..10108
                     /gene="rsmG"
                     /locus_tag="VIS73_13270"
     CDS             9485..10108
                     /gene="rsmG"
                     /locus_tag="VIS73_13270"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEY5764172.1"
                     /translation="MKPAESLAAGLADLSLPLAAEVQARLLAFGRLLQKWNRTYQLTA
                     QADDRALVAVHLLDSLAILPWVGTGHLADIGSGGGTPGIPLAIARPDLAVSLVEANGK
                     KAAFLQQAAIELPLPNAEVIAARVERWQPQERPTMVTSRAFAALADFVGLTRHLLAPG
                     GRWLAMKGQYPADEIAALPPGVVVDHVQPLRVPGIDAARHLVVLRES"
     gene            10109..10903
                     /locus_tag="VIS73_13275"
     CDS             10109..10903
                     /locus_tag="VIS73_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY5764173.1"
                     /translation="MARILAIANQKGGVGKTTTSVNLAACLAEVGQRVLMIDLDPQGN
                     ATTGCGIVKREALPTVYQILIGASTLDQARLKTEFGFDILPSNRELAGAEVELIEVTQ
                     REFRLRDALAQADGEYDFVLIDCPPALNMLTVNGLVAAQAVMIPMQCEYYALEGLTDL
                     VATLRKVRANLNPVLEIEGLLRTMYDPRSTLTQQVSLELESHFAGKVYNAIIPRNVRL
                     AEAPSYGKPVIAFDRNSKGAQAYLALANELLERREALNVSAAATLQ"
     gene            10912..11778
                     /locus_tag="VIS73_13280"
     CDS             10912..11778
                     /locus_tag="VIS73_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY5764174.1"
                     /translation="MSKMKMKGLGRGLDALLAGNDGEKAAAGRTDKLPVGKLEPGKYQ
                     PRTHMDQASLEELAASIRAQGLMQPILVRGIGGDRYEIIAGERRWRASQIAGLAEVPV
                     LVRDIPDEAALAMALIENIQREDLNPLEEAMGLQRLIDEFGMTHQQAADAVGRSRSAA
                     SNLLRLLQLAPPVQDLLLEAKIDMGHARALLPLGGGDQVRLAQQAANRGFSVREVERL
                     VQRLLQGEPEVKRKPVADADLVRLQEELADTLGASVAIKAGKGGRGRITVDYASLDQL
                     DGILERLRASGR"
     gene            11964..12290
                     /locus_tag="VIS73_13285"
     CDS             11964..12290
                     /locus_tag="VIS73_13285"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEY5764175.1"
                     /translation="MRQLLVGVVVALIAGWDAGFHAALSALIGAGIGVGAGLAYAWRA
                     VRPVHGDPKKAFQAQVLGEAYKIAVTLILFGAVFLNYREVAVLPLFSAYALTFVVYWM
                     ALLKQR"
     gene            12306..13151
                     /gene="atpB"
                     /locus_tag="VIS73_13290"
     CDS             12306..13151
                     /gene="atpB"
                     /locus_tag="VIS73_13290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004773235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY5764176.1"
                     /translation="MAAETLTATSYISHHLTNMAVCADSAKDAVGHCTGFWTVHLDTL
                     LVSGGLGLLVFVGLMKIAGRASTGVPAGWQNFFELIFSFVDQQVRDTYHGKSPLVTPL
                     AITIFLWVFIMNAMDLIPVDFLPMTAAWIGETFFGADPHHVYLKVVPTTDPNLTFAMS
                     LTVFVIMIGMNFKVKGAGGFAHEALTAPFGAKLAPVNLLFRIVEDIAKPISLALRLFG
                     NMYAGEMVFILLAVLMAGGSLWTPVGAVLSLGWALFHILIITLQAFVFMMLTIVYMSM
                     ASESH"
     gene            13216..13473
                     /gene="atpE"
                     /locus_tag="VIS73_13295"
     CDS             13216..13473
                     /gene="atpE"
                     /locus_tag="VIS73_13295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY5764177.1"
                     /translation="MSALLGNTAIAVAILIGAGALGTAIGFGILGGRFLEGAARQPEM
                     IPTLQVKMFIVAGLLDAVTMIGVGIALFLLFANPFIAVVKG"
     gene            13508..13978
                     /locus_tag="VIS73_13300"
     CDS             13508..13978
                     /locus_tag="VIS73_13300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY5764178.1"
                     /translation="MSINATLIGQAIWFGLFIWITMKYVWPPLQKAMADRQQQIADGL
                     AAAERGKHDLELAAKRSADSLREAKEKANEVIAGADRRGAQLIEEAKAAARVEAEKVI
                     ASAKAEIDQEVERARAALRDRVAELAVAGAERILKREVDAKAHGEMLAALKQEL"
     gene            13983..14519
                     /locus_tag="VIS73_13305"
     CDS             13983..14519
                     /locus_tag="VIS73_13305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY5764179.1"
                     /translation="MAELVTIARPYAEAAFRLAQEKKALAAWSQQLELLSAIAADAQM
                     KSCIGNPQLSAPQKSELFRSLAGDKMDGDAARLVEVLAKNERLAVLPQIAELFESLKG
                     EAEGVQDAVIHTAFPLSDAQQKAIVADLEQRYKTRLNAQVVVDQTLIGGVKIVIGDQV
                     LDTSVRGKLDAMATALKN"
     gene            14530..16068
                     /gene="atpA"
                     /locus_tag="VIS73_13310"
     CDS             14530..16068
                     /gene="atpA"
                     /locus_tag="VIS73_13310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY5764180.1"
                     /translation="MQLNPSEISELIKSKIQDLKISADLRTQGTVVSVTDGICRVHGL
                     TDVMQGEMLEFPGNTFGMALNLERDSVGAVVLGEYEHISEGDTVKTTGRILEVPVGPE
                     LLGRVVNSLGQPIDGKGPIGNKMTDKIEKVAPGVIWRKSVSQPVQSGLKAIDAMVPVG
                     RGQRELIIGDRQTGKTAVAVDTIINQKGQDMICIYVAVGQKASTVANVVRKLEENGAM
                     EYTIVVAATASESAALQYIAPYSGCTMGEYFRDRGQDALIIYDDLTKQAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVSEEWVEKFTNGEVKGKTGSLTALPVIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGVRPAMNAGISVSRVGGAAQTKVIKKLGGG
                     VRLALAQYRELAAFAQFASDLDEATRKQLERGRLVTELMKQPQYSPMSVAMMAVTLLA
                     VNKGLFDDVEVKSALEVEKQMQSYLKQKHADLISTIETKKDLDADAEQKLTKAIEEFK
                     ATLA"
     gene            16093..16956
                     /gene="atpG"
                     /locus_tag="VIS73_13315"
     CDS             16093..16956
                     /gene="atpG"
                     /locus_tag="VIS73_13315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEY5764181.1"
                     /translation="MPSGKEIRTKIKSVENTRKITKAMEMVAASKMRKAQERMRASRP
                     YGEKIRRVAAHLSHALTEYRHPFLVVREPIKRIGMITVTSDKGLCGGLNSNVLRLSLA
                     RFKEWQGEGKQVQVTAIGNKGFGFMQRLGAEVVSHVTGLGDTPHLEKLIGPVKVQLDA
                     YVRGDIDALFIVYTRFINTMKQEPVIEQLLPLSDDVVGSESAKWDYLYEPDAKSVIDD
                     LLTRYVEALIYQSVAENMASEQSARMVAMKSASDNAKNVIGELKLVYNKARQAAITKE
                     LSEIVAGAAAV"
     gene            16981..18384
                     /gene="atpD"
                     /locus_tag="VIS73_13320"
     CDS             16981..18384
                     /gene="atpD"
                     /locus_tag="VIS73_13320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY5764182.1"
                     /translation="MSQGNIVQCIGAVVDVQFPRDAMPKVYDALMLDKSEASSMVEAD
                     LTFEVQQQLGDGVVRTIAMGSSDGLRRGMKINNTGKGIAVPVGEATLGRIMDVLGRPI
                     DEAGPIGTDERREIHQSAPKFDELSPSVDLLETGIKVIDLVCPFAKGGKVGLFGGAGV
                     GKTVNMMELINNIAKQHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVSMVFGQMNE
                     PPGNRLRVALTGLTMAERFRDDGRDILFFVDNIYRYTLAGTEVSALLGRMPSAVGYQP
                     TLAEEMGRLQERITSTKVGSITSIQAVYVPADDLTDPSPATTFLHLDSTVVLSRDIAA
                     LGIYPAVDPLDSTSRQLDPLVVGQEHYGVARAVQGTLQRYKELRDIIAILGMDELSPE
                     DKLAVARARKIQRFLSQPFHVAEVFTGSPGKYVTLKDTIKGFKMIVEGECDSLPEQAF
                     YMVGGIEEAFEKAKTLQ"
     gene            18445..18870
                     /locus_tag="VIS73_13325"
     CDS             18445..18870
                     /locus_tag="VIS73_13325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY5764183.1"
                     /translation="MALRVHLDVVSAEESIFSGETDFAVFPGEAGELGIYPRHTPLLT
                     RIRPGTIRIKNPSSNDYELVYVSGGMLEVQPDMITVLADTAVRAADLDEAKALEAKRQ
                     AEEALRNRSSEIDYATAEAELAEAIAQLQAIEKLKRRGH"
     gene            19018..20544
                     /locus_tag="VIS73_13330"
     CDS             19018..20544
                     /locus_tag="VIS73_13330"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5764184.1"
                     /translation="MQPKARLPHAAEAVLYVRLPCWKIYPGGVVYVADYVHKNRPLVR
                     QHLLDLALVPRRKRRAALHECIDSFHPDIVAFSWRNMQSFGPHPEDDALDVVMNFDHA
                     RNPLRRLRAAGDALRIIYDYTAARLDNFAFMRLVRRRLPQARIVVGGTAVSIFGPYVA
                     EKMPSNSVVVVGEGEDSMLSIVDGVEVPLGETYRKDAEGRITHHERVANFELAELGAV
                     DFDYITTIFPGFGAYLDDDAVGVNTKRGCPFQCHFCLYNRIEGPNQRYRDPAEIVDEI
                     EALNRQHGVRRIWFTDAQFCSTKRSTEHVEQVLDELLERKIKIGWSGYLRLNHLTPSI
                     ARKMIASGIESIDLSFAGTQEVIDSLTLGYRLDQQMDAFRMIRESGYGEQKIKLYMPL
                     NAPGETAETLRRTIAKVQELYALFGRDNVLPFIFFIGVQPGTPVEKRLIDGGYLPADY
                     DPLTLNPFVIKKLLYNPRPLGTLIGRAYLEAVDSLNRNQDEYIGRATLDVLARELGGA
                     "
     gene            complement(20566..21144)
                     /locus_tag="VIS73_13335"
     CDS             complement(20566..21144)
                     /locus_tag="VIS73_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764185.1"
                     /translation="MIALLQSLLDRLLADQAEARQRLARHTGRRLRVGVLGGAATLAI
                     DEGGALRIVDLHGDPAADLRVTISAESLTALPAGDPLRGARIEGAADLADDFSAAART
                     LRFDPADWLAPFTGDIVANRIGEGLKALGAVGADALSRAEAALRDRAAADSLPLLRHA
                     EAAGFDAELRQLFDAVESLDRRVGRLAQSGGR"
     gene            complement(21158..21895)
                     /gene="ubiE"
                     /locus_tag="VIS73_13340"
     CDS             complement(21158..21895)
                     /gene="ubiE"
                     /locus_tag="VIS73_13340"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEY5764186.1"
                     /translation="MSEQTTHFGYRTVDESAKAAHVREVFDSVATRYDLMNDLMSAGL
                     HRLWKRFAIGQSGVRAGQRVLDIAGGTGDLAQAFAERVGTRGEVWLTDINHAMLSCGR
                     DRLVDSGVLLPVAQCDAEKLPFASGYFDCVTVAFGLRNMTHKDLALAEMYRVLKPGGR
                     LLVLEFSKVCKPLAPAYDFYSFQVLPRLGDLVAKDSESYRYLAESIRMHPDQESLKAM
                     MEAVGFERVDYFNLTAGVVALHRGFRF"
     gene            complement(21909..22358)
                     /locus_tag="VIS73_13345"
     CDS             complement(21909..22358)
                     /locus_tag="VIS73_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEY5764187.1"
                     /translation="MAGLENNPPIPEEVLLRTHSRCLDLVYPDGKRFSLPFEYLRVYT
                     PSAEARGHGPGQETLQVGKREVTIDDLEAVGNYALRPHFSDGHDSGIYSWDLLFNLAS
                     HQDELWKNYLEQVEAAGASREAGANVSPKAAGGCGSGSCGSGGCGSH"
     gene            complement(22830..23267)
                     /locus_tag="VIS73_13350"
     CDS             complement(22830..23267)
                     /locus_tag="VIS73_13350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY5764188.1"
                     /translation="MSKVTADSDCPLCVAAGGALVWQNALCRVVAVDDADYPGFVRVI
                     LNRHQSEMTDLQAAERDALMAVVWAVESVLRESLQPDKINLASLGNVVPHLHWHVIPR
                     WRDDRHFPNPIWGEPMRKAAARPVDAAALAAALSGRLDRLTAQ"
     gene            complement(23264..27115)
                     /locus_tag="VIS73_13355"
     CDS             complement(23264..27115)
                     /locus_tag="VIS73_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEY5764189.1"
                     /translation="MTARLREIPYNYTSFSDREIVIRLLGADAWSIVDELRRERVTGR
                     SARMLYEVLGDIWVVRRNPYLEDDLLANEKRRDALIGALHHRLNEVDKRRAGNERVAS
                     LLAAARNAVRDFERWFETTETLRRRTLRVLSRHTRRDNICFDGLARVSHVTDATDWRV
                     EHPFVVLTPDTEEEIGPLVRDLISLGLTIIPRGGGTGYTGGAVPLTPFSAVINTEKLI
                     DIGPVEELTLPGCETPYATIRTSAGVITERVAEAASGAGRVFAVDPTSASASCIGGNI
                     AMNAGGKKAVLWGTALDNLAWWKMVTPDGDWLEVERLKHNFGKIHEQETVEFRVTRYD
                     STGKKRLAGEILTMPGAACRKTGLGKDVTDKFLGGLPGVQKEGCDGIIVAARWVLHRM
                     PPVLRTVCLEFFGQVREAVPAIVEITDHFKPGGAAHAAGVQLAGLEHLDERYVKAVGY
                     TTKAKRHGRPKMVLIGDIVGADEDSVMRAASEVVRMCNARGAEGFVATTPEQRKRFWL
                     DRSRTAAISRHTNAFKINEDVVIPLPRMGDYCDGIERINIELSIANKIALCDALAEFL
                     DGELPLHLGDAGVDRAALIGDRRTTALDAVGAARARWQWLLDSLDLPLAEAEARFSEF
                     GIEAAELSNRAANPRLFHRLQDYSVRVSWKKELKPQLKEIFDGVFFRPTLERIDAIHQ
                     EVLRGRVFVALHMHAGDGNVHTNIPVNSDNYAMLQTANTAVARIMALARSLDGVISGE
                     HGIGITKLEFLAENEIAPFRAYKAKIDPEGRFNKGKLLEGGDLANAYTPSFALLGVES
                     LIMEQSEIGRISDSIKDCLRCGKCKPVCSTHVPRANLLYSPRNKILATSLLTEAFLYE
                     EQTRRGVSLQHFDEFSDVADHCTVCHKCLNPCPVDIDFGDVSVAMRNFLREHGKKKWN
                     PGTAAAMAFLTVKDPATIKLIRKVMIEWGYQAQRLGHRLAKSFGLIEPSVKRPAATLG
                     RPPLKAQIIHFLNKPMPGGLPKRTSRALLDIEDDRIVPVIRDPKKAGDDAEAVFYFPG
                     CGSERLFSQVGLATQAMLYEVGAVTVLPPGYLCCGYPQTSAGEEVKGRKITTDNRVLF
                     HRVANTLNYLDIKTVIVSCGTCMDQLQKYEFEKIFPGCRLLDIHEYLMEKGVKLDGVD
                     GVRYLYHDPCHTPMKTHAPLTVANTLLGQPVVLSDRCCGESGTLAVTRPDVSTQVRFR
                     KQEEIEKGDAKLRSDGFAGPVKLLTSCPSCLQGLHRYVGDAAVEPDYIVVELAKRLLG
                     DDWLVDYVDKANAGGIERVLV"
     gene            27402..29435
                     /locus_tag="VIS73_13360"
     CDS             27402..29435
                     /locus_tag="VIS73_13360"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5764190.1"
                     /translation="MRSPTLIHKFLRMFLPMVALAVAVVLVYGQGELERQISRLRNHD
                     ALVIGLGAGALAGRIETIHRDLAFLSASHSLREAINRPSAQHLAELAEDFVIFSRSKG
                     VYDQIRWIDETGFERVRVELTNGQPELVPQERLQDKRQRYYFVATMALRPGEIFASPL
                     DLNVEQNQIEVPYKPTLRLATPVADAQGRLRGIVIVNYLGRDLLETFDRETKALAGRS
                     MLVNQDGYWLKGTTPDDEWGFMFDRSELSLAARYPRAWQHISSVDRGQVALEDGIWSW
                     QTVRPFSSRGLTSVETITAQQHVWKAVSHLHDSDLMGMARTVWLRLSAVMLLLAAGVA
                     YGCWKLVLAWDARAEADNRLRSLNVDLEATVESRTEALRLSERRFRDVAHISADWIWE
                     VDAEARYVYASESVEELLGYRPEEIIGKTAFDLMPPDEARRVRVAFAEIAEARRPFRD
                     LENIVLGKDGKAHITLTNGMPILGADGALLGYRGVDRDITAQRQMEQQIRQMAFIDGL
                     TGLPNRRLFADRLEIALAASRRSGERGALLFLDLDKFKALNDRAGHEAGDLLLVEAAR
                     RLQGCVREMDTVARLGGDEFLIILGGLDADDKRAAEQVKHVAEKIAAAMGAPYLLKLA
                     DKTVEHHCTASIGATLFLGHEVDGEELLRRADAAMYEAKAAGRAAIRFSKHSP"
     gene            complement(29541..29963)
                     /locus_tag="VIS73_13365"
     CDS             complement(29541..29963)
                     /locus_tag="VIS73_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEY5764191.1"
                     /translation="MSDFLDAETSLAGLFALSFLAATLLPGGSEIALYAFVKGHPDRV
                     ADALVLATVGNTLGGMTSYACGRFLPKWQQLERVPHMDLVRRYGSASLLLAWAPLIGD
                     LLCVAAGWLRTHWLAAISFMAVGKGARYWLLAQGAAAL"
     gene            complement(29960..31015)
                     /locus_tag="VIS73_13370"
     CDS             complement(29960..31015)
                     /locus_tag="VIS73_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HEY5764192.1"
                     /translation="MPAAETPGWKLQFASDNTAGACPEALAALAAANAGFVASYGSDA
                     WTHQVCDRLRTVFETDCDIYFVFNGTAANSLALASLCQSYHGVICSESAHVETDECGA
                     PEFFSNGSKLLTAPQREGQISPESVAALVKKRSDIHFPRPRAVSLTQATEFGTVYSPA
                     QVAAIAAVARADGLHVHMDGARFANAVAGLGVSPADLTWRAGVDVLCFGGTKMGLPIG
                     EAVVFFDRRLGEEFAYRCKQAGQLASKMRVLSAPWLAMLDGDTWLHRAAHANAQAQAL
                     AARFAAIPGVRLAHPVQANGVFAELPAGAGERLRHAGWMFHDFIGGASRFMCSWATED
                     EAVDALANDLARAVAAT"
     gene            31108..>31286
                     /locus_tag="VIS73_13375"
     CDS             31108..>31286
                     /locus_tag="VIS73_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEY5764193.1"
                     /translation="MNAPAPVLNDYLERILNARVYDVAVETPLDPAPGLSERTGNTVL
                     LKREDMQPVFSFKLRG"
BASE COUNT         5614 a        10112 c        10157 g         5403 t
ORIGIN      
        1 agccgcgcca cctgcggacc gttttccgca ttgggcagtt cgccggatgc gccgccaagc
       61 gagcacggcg gcgcctgact atgacgaatg tccaataggc agcgcgctcc cccaacccta
      121 gaatgaccgc ttcatcgaaa ccatgcaggg aggcgccagc gtgcgccctt ggtggacacc
      181 actgcgggga gtgatgctag cgctgacggc cattaccgcc atcgcgctgg ttcgcctgtg
      241 gctagccggc accggtgcgc acgatgccga gcgtgccgac accgaagccg cagcaacacg
      301 gcacgcgacg accatagcgg cggtgaccgc tgaaaccctg cgctcgcgac tcgagtcgct
      361 cgaccacacg ctgcggcaga tggcgatcgc ctacgccgaa catcagcaga taccgctcga
      421 agatctgcgc attaccagcc tgtcgcagcc gccggggctc gccctcaact tcgttcgcat
      481 cgacgcccgc ggcttcgtgc gcgacgacga ataccagacc gagcgggctg gccaaccgca
      541 gcgctacctc ggtgatctgc cgctcttccg gtaccacgcc tcgaacccag acgacgcgct
      601 tcgcgtcgac ccaccggcga aatcgcgtct cgttgatcgg tggaccatac cggtctcccg
      661 agtcctccgc gatgacgaag gacgattcgc cggtgtcgtc ctccttgaac tctcacccga
      721 gcaactgtcg gcggatttcc gcgcgctcgg gctcgattcg aacgacgtca tcggcatcat
      781 tcacgccgac ggttcctacc ttgcccgcag ttatcggctg gacgaggcgc tcggcaacaa
      841 ggttcgcggc gaccacgcct acctgtttcc cggcgccgcg ctcagcggca gcttccgcgg
      901 gcagggtggc gtcgatggtg ttgaacggat ctgggcgtat cagcgactgc ccggcttcga
      961 gctcagcgtg gtggtcggcc tcgacctaac cggcccgagc cactccatgc tcgctcgcca
     1021 cgagagcgag cgcgacaaca tgatcctggc gaccgcgatg gctgtcgcgc tgctgggcgc
     1081 gctgctggct ctcctctggc gcctcgatgc cagcctgcgg gcactgcagg cctcggaagg
     1141 ccgactcaag cgggcgctcg aaggcgcgtc cgaggtcgcc tgggaatggc gcgccgactc
     1201 cgatcgggtc accctgctcg gcaactgccg cctgttccta ggcatcgccg aaccgagcat
     1261 cgagctcgag cttgccgctt ggcgggggcg cgtacatccg agcgacctgc cgtcgctgac
     1321 cgatgcctgg cgcgagcacc tggccgggca ggccgacgag ttcgaggccg aataccgcgt
     1381 ccttggcggc gagaacagct accgctgggt actcgatcgc ggtctcaccg agcatcgtga
     1441 tgcgcagggc cggccgtcga gaatcgccgg cgtgctaatc gacatcgatc cgatcaagcg
     1501 cgcccagctg acgatcaccc gcctgtcagc gcgctatcag cagctgttcg cgagcgccaa
     1561 cgaaggcatt tacgtggtca acacgcaggg cgaaatcgaa atgatgaacc gcgcggcgca
     1621 gacgctgacc ggctggcggg aagaagaagt tctcggcaag caggcgcatg cgttgttcca
     1681 cgaggcgctc gccggccatc ccgaccatag ctggccaacc tgtccgctca acatggcgat
     1741 gaagaccggc gacgtgcgcc gcggcgagcg catgctctac tggcgccagg acggctcggc
     1801 gatgcccgtc gagctgtcga tcgggccgct gctcgagggc gacgaatgcg acggtgcgat
     1861 cgtcgttttc agtgacatca ccgagcgcct cgccaccgaa cgcaagctcg agctgctggc
     1921 gaccaccgac gaactgaccg gcctagccaa ccgccgccgc ttcgtcgaga ttgccgagcg
     1981 ggacctgcgc cgcgccgcgc gcgaaggcac accggcaacg ctgatcatga tggatctcga
     2041 ccatttcaag accgtcaacg accgtttcgg ccacgcgatg ggcgaccgcg tgctcaaggg
     2101 cgttaccgac gcctgcgccg cccagctgcg cgccgtggac gttttcggtc gtcttggtgg
     2161 tgaggagttt gccgtgctgc tcgccaacat cgaacccgag cgggcgctcg aagtcgccga
     2221 gcgcatgcgc gtggcggcag ccgaacgtga gattcgtgac ggtcagcgtt cggtcgcggt
     2281 gaccgttagt ttgggcctcg cccatgctgg ttttggcgaa acgctggaca gcctgctcgc
     2341 gcgcgccgac gcggcgcttt atggcgccaa ggaaagcggc cgcgatcgca tcgtcgtcgc
     2401 cgacccagcc ggcgccgaaa ccgcgcaagc agacgcatcg gcatagcgcc tttcccagcc
     2461 gttctcgttg gcgtcggctg ccggtcaggc cggcagcctt tgttattatc ggcggtcggt
     2521 tcacagccgt tgtatccgcc aaggagcatg aggatggaaa gtttggcccg agcgctattg
     2581 ctgtcggtcg ccttggcgtt tgccggtgcg gcggcggcgc aaggtggcgg tgacagcgat
     2641 gcgcagcgtc gcctgctgga gcaaaagctg cggctggtcg agatgctgct caagtcgccg
     2701 gccgcccagg cgcgcgaagg cgaggcggcg gcgagcgtcg ccgaaggtcg cgaggccttg
     2761 gcgcgggcac gcacggcgct gagcgaggca cgactggacg aggcggcaac gctcgcagac
     2821 caggcgctga agagcgtatc ggcggcgtcg cgccgcggtg catcggcgcc gctctcggag
     2881 agcgcacagc gcaagtcgct cgacgatctt ggcagtcagc tggcctcgta ccgcagctcc
     2941 attgtcgaac tcggccaaga ccccaagctc ggcgacgcgg cccgcgatct gctcacgcgc
     3001 atcgacagtg cccgcgatga atccaaccgg ctcgccgccg atggccagct cggcgacgcc
     3061 aaccggacgc tggccgccgc ctaccgcctc gccgtcgaag agatttcgcg cctgcgtcag
     3121 ggtcaggaag tggtgatgtc gctcaagttc gattcgccgg ccgacgaata cgcctacgat
     3181 cagcgccgct tcgcgtcgaa cgaaatcctg gtcgagcaga tggtcgcgca gggcaaggcc
     3241 gacggcggtc gccagatgct ggtcgacaag ttctctgccg acgcacggcg catccgcgac
     3301 gaagccgagg ggctcgccgc ggccggcgat cacccgggcg ctgtcgtcct gatggaaaaa
     3361 gccgtcggcc aactgaatcg cgccctgcaa acgatgggcg tacccgtttt ctgaggagcc
     3421 aagatgattt ccctaacccg acgatccggc gtgttcagtc gtgccacgct ggcgctgttg
     3481 gcgattctgg cggccggccc gctcttcgcg gcggccgctg acgacgcgct ggcgacgatc
     3541 aacatcgccg gcgaacaacg gatgctgtcg cagcgcatcg tcaaggccta cctgcagctc
     3601 ggcctcaacg tccagccgct cgcggcgaag acgcaactcg acagtgcgat ccgccgcttc
     3661 gaggccaatc agcagttgct cttgcctgca attgccggcg accccgattc gcggcgtgcc
     3721 ggcgaactgc tgacgaccgc ctgggaacag atgcggccgc tgactagcgg cgcgccgacg
     3781 ctcgagaacg cgcagcgcct gtcggcctac ggcgagactg tgctcaacgc cgccgaaatg
     3841 ctggtcaacg tctacgccga ccgttcgggt gtcgacgagc atcgcctgct gcggctgatg
     3901 gctcgccagc ggatgctctc gcagcgcatc gccaagacct acctgctgcg cagctggggc
     3961 gaccagtcgg cgcaattgcg gtaccagagc gaagacgcga tcatcgcctt cgagggtgtg
     4021 ctgacgatgt ttcagagtcg tcgcgacgag ggaccgatgc tgcgccagga gcttgatgaa
     4081 ctggcgctgc agtgggagtg gatgaaggct gcgctcgagt ccgatggcgc gctgagttac
     4141 cgcctgatcg tcgccgagtc ggccgatgca atcctcgcca gcgccgatcg cctggcccag
     4201 cagtaccagc gcctgctcgc tgctcgttga aagggaacgc cgaatgacca tgcaacgccg
     4261 ggattttctc aagggcctcg ccaccggagc aagcctcgcc gccggcggcc tgccgcgcct
     4321 cgcgcaggct gcaccgatgg tgacttcgct caatgccgga gcggtcgaac agatcctcac
     4381 gcccggcggt ggcgtcagta atctctgggg atacaacggc accgtgcccg gacccctgct
     4441 gcgcttccgc aagggtgaga ccgtgcgcat cccggtgcgc aatggcctgc ctgccgacac
     4501 caccgtgcac tggcatggcc tgcgggtgcc caacgcgatg gatggcgtcc cctacgtcac
     4561 gcagaatccg atcaagcccg gcgagacctt tcagtacgag ttcacgattc ccgattcggg
     4621 cagcttctgg taccacccgc accagtcgag ttacgaacag gtgccgcgcg gcctctacgg
     4681 tccgctgatc gtcgacgagg aaaagccgat cgagatcgac caggaagcga tctgggttct
     4741 ctccgacgct aagctcgaag ccaacggccg gcaggtcgag gatttcgggc gcgtgcttga
     4801 tctcgccaac gaagggcgcc tcggcaacgt cgtgctgatc aacgggctgg cggccggcgc
     4861 cgagcgcagc ctcaaggtgc gctcgggcga tcgcgttcgc ctgcgcctca tcaatgccgc
     4921 cgcgtcgcgc atcttcaagc tggcctttgc cggacacgtc gtgcaggcga tcgccttcga
     4981 cggccaggcg gtgacgccgc atccgctcga ggagatctat atcggccccg gcatgcgcat
     5041 cgacctgatc atcgactgca tgcacgcgcc gggttcgagc ttcaccatcg ccaaccgtga
     5101 tcgtcgctcg cttggcgacc tggcgcgcat cgtctacacg cccgagaagc cgcggcggag
     5161 caagccgatg aaggcgccga tcgcgctgca gcccaatcgc ttccccgaac ccgacctgaa
     5221 aaaggcaatc gaccactacg tcgtcttcga gggcggcatg cgcggcgcgc cggtgatcgg
     5281 ccaggtcgac ggcaagccgg cgaagatcca ggacatcatg gagaagtacg ggttggcgtg
     5341 gacgatgaac ttcagcgccc agcacgagca cgcgctgatg cacgagccgc tgttctacct
     5401 gaagaagggc gatcacgttc tgctgcgcat gatcaacgag accgacttcg tgcatccgat
     5461 gcacctgcac ggtcatttct tccgcgtcgt cgccatcgac ggcgtcaaga ccgtgcatca
     5521 ggaatggcgt gacaccgtga tgatgggacc gcggcagacc gtcgacgtcg ccttcgttgc
     5581 cgacaacgtt ggcgagtgga tgtaccactg ccacattctc gaccacgccg ccggcggcat
     5641 gatgggcacg atcgctgtcg agtagcggcg gcccgcggtt cagcgggccg ttcccgagcc
     5701 tcttagcgaa tcggcgacct gctggcgcaa ggcagcggcg ttgatgccgt atttttgagc
     5761 aacggcttgg aacagctcgc cggcggcaat tacccgtgaa tgacgtggat cgcgccgttg
     5821 cgcgcggagc aaatagccga gcgcccgttc ggcaagcgga tcctgccagc cttgccgatc
     5881 gagcagcgta aagatcgcgc tggtggcatt gaccagaaac tgaaggttgg gaattcggtc
     5941 ggccgctgcg cagagcaggc gcaccgattc ggccagatcg ccctggcgag ccgtgagcac
     6001 gccgcggttg ttgatgtcga tgatctcgcg cgagacctcg tcgagaatcg ccaggccagc
     6061 gtcggtgttc tcggttcggt cgaagatgtc gcgcattcgg tcgatcaggg cggtattctc
     6121 ctgatgctcg gcggcgacct gacgcagcac ctggccggct ttctcggctt cgccgctggc
     6181 caggcaggca tgcgcgagat cgacggcgat cttctcgggc agggcaaagc cggccttgtc
     6241 cttttcgctg agcccctcgg cggcgctcag cgctcgatca agcgctgcac gtgccttgtc
     6301 gggttcgccc tcctcggcgt ggcagaggct ttcgaccacc gaggcggcga cctccgtcgt
     6361 cgggtcccag cgccggtctc ggcgcatctc ctgcgcaacg ctgcgcgccg cagaagcctt
     6421 gccaccatca agcagcgcac gtgataggtt ggcgtaatcg tcggcggtcg acagcgacga
     6481 tccgcgggtg cgggtgagca ctgcctggaa ggccttttcg gccagctcgt ggtggcccga
     6541 ccttgccgcc acctcaccga cgatgcgctg gcggccggtg ttgtgcggcg agatctcggc
     6601 ggcggaggcg agcacgctgc gcgcgtcgtc gaggttgccc cgctcctcga gcagcgccgc
     6661 aaggaaatcg tgggcagcga gaaagtgcgg gtgatcacgc agcagctcgc gcgccagcgt
     6721 ctccgcctcg tcgatctgcc cggtcgcgcg tagcgccatg gccaggccca tcttcgccca
     6781 aggcagcggc cgcgttgcga ggacctcgcg cagcagcgcg atcgcctcgt cgtggcggcc
     6841 cagcaggttg agaatctcgc ctttgtggcg cagcgtttcg agatagaaat cgttgtcgcc
     6901 ggcgagcagt ttctcgcagg cggcgagcgc tctgccgtag gccgcggcgt cgagttcacg
     6961 caagatcggc gagagcacct tcttggcgta gagcgcgtga gcgaggcgac tctgcagctg
     7021 ttcggcggtg aagggtttga ccaggtagtc gtcgggggtc agctcggcga ccgagaccac
     7081 gttgtggtaa ccgcgctcgc tggtgacgat gaagaacacc gtcgacagcg gtatcaagtg
     7141 cttgacgcgc agctcctcga gcagctgctg gccgtcgcgg ccgtcgccga gcaggtaatc
     7201 ggagaagacc acatcgaagg ggctggcgcg gaccagccgc agggcctcga ccgaggaact
     7261 cgcgccgtgg accttggtga tcgacagcga ggcgagcatc aggcgcagcg aatcgcgggc
     7321 gttcgaatgc cggtcgacga tcagcacgcg cagaccggtc aggtcggcgc gaaccagatg
     7381 ctcggcggcg gcagcgttct tgggcacggc aggctcgggc gaagagaggg gcgattgtcg
     7441 ggccagccgt gcgtccatga caaccggcaa acgcggcccg ggctttcgcc acgcggctcc
     7501 gggcggcacg ccgcgagggc gaaaactgtg cgatttcagc aacttcgcgt atcattccgg
     7561 tccgatcgac cccgcggaag cactcgcgca tgcagtatcc caatcgtttc gatgtgatcg
     7621 tcgtcggtgg tggccatgcc ggcaccgagg ctgcgctcgc cagtgcgcgc agcggcgcac
     7681 gcaccctgct gctcacgcat aacctcgaga cgctcggggc gatgagctgc aatccgtcaa
     7741 tcggcggcat cggcaagggg cacctggtca aggaggtcga tgcactcggc ggcgcgatgg
     7801 cggcggctac cgacgaggcc ggcatccagt tccgcatcct caacgcctcg aaggggccgg
     7861 cggttcgcgc gacgcgcgcg caggccgatc ggacgcttta ccggcaggcg attcgcagcc
     7921 ggctcgagaa tcagccgaat ctctggctgt ttgcgcaggc cgtcgacgat ctgatcgtcg
     7981 acggggatcg agtctgcggg gcggtgacgc aggccggcgt tcgtttcgcg gccgacgccg
     8041 tggtgctcac cgccggaacc ttcctcaacg gactgatcca catcggcctg cagaaccatc
     8101 ccggcggccg tgccggtgat ccgccggcgg tgtcgctggc ggccaggttg aaggagctga
     8161 agctcccgca agggcggctg aagaccggca cgccgccgcg ccttgacggc aagagcatcg
     8221 atttctcgct catgcaggag cagcactcgg acgcgcccct gccggttttt tccttcctcg
     8281 gcagcgccga ccaacatccg cagcaactgc cgtgctggat caccgatacc aatgagcgga
     8341 cgcacgagat catccgcggc ggactcgacc gctcgccgat gtatacgggc gtcatcgaag
     8401 gggtcggacc gcgctactgc ccgtcgatcg aagacaagat tcaccgcttc gccgacaagg
     8461 cgagccatca cgtcttcctc gaacccgagg ggctcgacac ccacgaggtt tatccgaacg
     8521 ggatttcgac ctcgttgccc ttcgacgtcc agcttgagat ggtgcgctcg atccgcggcc
     8581 tcgagaactg ccacatcctg cgccccggtt acgcaatcga gtacgactac tacgacccgc
     8641 gcgggctcag ggactcgctg gaaacgaagg cgattggcgg cctcttcttc gccggccaga
     8701 tcaacggcac aaccggctac gaggaggcgg cggcgcaggg cctgctcgcc ggactcaacg
     8761 cggcgcggcg cacccgcggc gaggcgccgt ggtgcccgcg ccgcgacgag gcctacctcg
     8821 gcgtgctggt cgacgacctg atcacgcgcg gcgtctccga gccctaccgg atgtttacct
     8881 cgcgcgccga attcaggctc agcctgcgcg aagacaatgc cgacctgcgg ctaaccgaaa
     8941 ttggccgtcg cctcgggctc gtcgacgatc agcgctgggc tgccttcagc cgcaagcgcg
     9001 aggcgatcga gcgcgagagc gcgcgactgc gcggcacctg gctgacgccg gcacggcttg
     9061 gcgaagccga ggcagcgcgc gtgctcggca agccgatcga acgcgaatac acgctgctcg
     9121 acctgctgcg ccggccgggc atcgcctacg cgtcgctgat gaccctgccc ggcgcaggac
     9181 ccggcgaggc cgacccgcag gtcatcgagc agctcgagat tgccgcccgc tatcagggct
     9241 acatcgtcag gcagcaggac gaaattgccc gggtcgccgc cagcgagacc acggcgatcc
     9301 ccgatgatct tgattacgcg caggtgcgcg gcttgtcgaa ggaagtgcag cagcgcctcg
     9361 aacagcagcg gccgcggacg atcggccaag ccgggcgcct gcagggcgtt accccggcgg
     9421 cgatttcgct gctgctgatc catttgcgcc gggtttcggc cacgcgcaat atcgaccgcg
     9481 ccgcatgaaa cccgccgagt cgctcgccgc cggcctggcc gacttgtcgc tgccgcttgc
     9541 cgccgaggtc caggccaggc tgctcgcctt tggccggctg ctgcagaaat ggaatcgcac
     9601 ctatcagctg acggcgcagg ccgatgatcg tgcgctggtc gccgtgcatc tgctcgattc
     9661 gctggcgatc ctgccctggg tcggcaccgg tcacctcgcc gacatcggca gcggcggcgg
     9721 tacgccgggc atcccgctgg cgatcgcccg gcccgatctg gccgtgagtc tggtcgaagc
     9781 gaatggcaaa aaggcggcct ttctgcagca ggcggcgatc gagctgccgc tgcccaatgc
     9841 cgaagtcatc gctgcgcggg tcgagcggtg gcagccgcag gagcggccga caatggtaac
     9901 ctcgcgggct ttcgccgcgc tggccgactt cgtcggccta acccggcacc tgctggcgcc
     9961 gggcggacgc tggctggcga tgaaggggca gtatccggcc gacgagatcg cggcactgcc
    10021 gcccggcgtc gtcgtcgatc acgtgcagcc gttgcgggtg ccgggcatcg atgccgcccg
    10081 gcatctggtc gtactcaggg agagctaagt ggcaagaatt ctcgcaatcg caaaccagaa
    10141 gggtggcgtc ggcaagacca cgaccagcgt gaacctcgcc gcctgcctgg ccgaggtcgg
    10201 ccagcgcgtg ctgatgatcg acctcgatcc gcagggcaac gcgacgaccg gttgcggcat
    10261 cgtcaagcgc gaggcgctac cgaccgtcta ccagatcctg attggtgcct cgacgctgga
    10321 ccaggcccgg ctgaagaccg aattcggttt cgacatcctg ccgtcgaatc gcgagctcgc
    10381 cggtgccgag gtcgagctga tcgaggtcac gcagcgcgag tttcgtctgc gcgacgcgct
    10441 ggcgcaggcc gatggcgagt acgacttcgt gctgatcgac tgcccgccgg cgctgaacat
    10501 gctgaccgtc aacggactcg tcgcggcgca ggcggtcatg atcccgatgc agtgcgagta
    10561 ctacgcactc gaggggctga ccgatctggt cgcgacgctg cgcaaggtgc gcgccaacct
    10621 caacccggtg ctcgagatcg aaggcctgct gcgtaccatg tacgacccgc gcagcacgct
    10681 cacccagcag gtttctctgg agctggagag tcactttgcc ggcaaggtct acaacgcgat
    10741 tatcccgcgc aatgtgcgct tggccgaggc accgtcctac ggcaagccgg tgatcgcctt
    10801 tgatcgcaac tcgaagggcg cgcaggccta tctggcgctt gccaacgagc tgctcgagcg
    10861 ccgcgaggcg ctgaacgtct cggctgcggc gacgctccag taataccaag gatgagcaag
    10921 atgaagatga agggactcgg ccgcggcctc gatgcgctgc tcgccggcaa cgatggcgag
    10981 aaagccgccg ccggtcgcac cgacaagctg ccggtcggca agctcgaacc gggcaagtac
    11041 cagccgcgta cgcacatgga ccaggcctcg ctcgaggagc tggcggcgtc gatccgcgcg
    11101 cagggattga tgcagccgat tctcgtccgc ggcatcggcg gcgaccgcta cgagatcatc
    11161 gccggcgaac gccgctggcg cgcctcgcag attgccggcc tcgccgaagt gccggtgctg
    11221 gtccgcgaca tccccgacga ggcggccttg gcgatggcgc tgatcgagaa tatccagcgc
    11281 gaggacctca atccgctcga ggaagcgatg ggcctgcagc gcctgatcga cgaattcggc
    11341 atgacccacc agcaggctgc tgacgcggtc gggcgctctc gctcggcggc gtcgaacctg
    11401 ctgcgcctct tgcagctcgc gccgccggtt caggacctgc tgctcgaagc caagatcgac
    11461 atggggcacg cccgcgcgct gctgcctctc ggtggcggcg atcaggttcg cctggcgcag
    11521 caggccgcca atcgcggctt ctcggtacgc gaagtcgagc ggctcgtgca gcgactgctg
    11581 cagggcgaac ccgaggtcaa gcgcaagccg gtggccgacg ccgatctggt tcgtcttcag
    11641 gaagaactgg cagacacgct cggtgctagc gtcgcgatca aggccggcaa gggcggtcgc
    11701 ggccgaatca ccgtcgacta tgccagcctc gatcagctgg acggcatcct cgaacgcttg
    11761 cgtgcctcgg gccgctagcc ttccgggcta tccagccggc gaaagctggc gtgttgcact
    11821 gcgtcatgac gctgtcgcaa gactctgaaa taaataacca ttgcaccctg tgcgcccgcg
    11881 gcaattgact cttatataag acctgatata ctcgcggggt tttttcgggg tctgtcttgc
    11941 cgccaccggt ccgctgggtt ctcgtgcgcc aactgcttgt tggtgtcgtg gttgccctca
    12001 tcgccggctg ggatgctgga ttccacgccg cgctgtccgc actgatcggt gccggaatcg
    12061 gagtcggcgc gggactggcc tacgcgtggc gggcggtgcg tccggttcat ggcgacccga
    12121 agaaagcatt ccaggcgcag gtccttggag aggcgtacaa gattgccgtc acgctgattc
    12181 tgttcggcgc cgtatttttg aactaccggg aagtggcggt tctgccgctg ttttctgcct
    12241 acgcactcac gttcgtcgtc tactggatgg cgctgctgaa acaacgctga tcgactggga
    12301 acatcatggc tgccgagacg ctcaccgcaa cgagttacat ctcccatcac ctgaccaaca
    12361 tggccgtctg cgccgacagc gccaaggatg ccgttggcca ctgcaccggt ttctggaccg
    12421 tgcatctgga caccctgctg gtttccggcg gcctcggcct gctcgtcttc gtcggcctga
    12481 tgaagattgc cggtcgcgca tccaccggcg tgccggctgg ctggcagaac ttcttcgaac
    12541 tgatcttctc cttcgttgac cagcaggtcc gcgacaccta tcacggcaag agcccgcttg
    12601 tgacgccgct ggcgattacg atcttcctct gggtcttcat catgaacgcg atggacctga
    12661 tccccgtcga tttcctgccg atgacggccg cctggatcgg cgagaccttc ttcggagccg
    12721 acccgcacca cgtctacctc aaggtcgtgc cgacaaccga ccccaacctg acctttgcga
    12781 tgtcgctcac cgttttcgtg atcatgatcg gcatgaactt caaggtgaag ggcgccggcg
    12841 gcttcgcgca cgaagcgctg accgcacctt tcggcgccaa gctcgcgccg gtcaatctgc
    12901 tgttccgcat cgtcgaggac attgccaagc cgatctcgct ggccctgcga ctgttcggga
    12961 acatgtacgc cggcgagatg gtctttatcc tcctcgccgt actgatggcc ggtggttcgc
    13021 tgtggacccc ggtgggtgcc gtgctctccc tcggttgggc gctgttccac atcctgatca
    13081 tcacgctaca ggccttcgtc ttcatgatgc tgacgattgt ctacatgtcg atggccagcg
    13141 agtcgcattg attttctgtt ccaacctttc ttgatcaaca cactgtctca ttaggagaaa
    13201 caaatggaag ctgctctgtc cgcgctgctc ggtaacaccg ctatcgccgt cgccatcctg
    13261 attggtgccg gtgccctcgg taccgctatc ggtttcggta tcctcggtgg ccgttttctc
    13321 gaaggcgctg cccgtcagcc cgaaatgatc ccgaccctgc aggtcaagat gttcatcgtc
    13381 gccggcctgc tcgacgcggt gaccatgatc ggcgtcggta tcgcgctgtt cctgctcttc
    13441 gccaacccgt tcatcgccgt cgtcaagggc tgagcgtttc cgatcaactc acgaccagga
    13501 ggttgacgtg agcattaacg ctacgttgat cggccaggcg atctggtttg gcctcttcat
    13561 ctggatcacg atgaagtacg tctggccgcc gctgcaaaag gcgatggccg accgccagca
    13621 acagattgcc gatggcctcg ccgccgcaga gcgcggcaag cacgaccttg aactggccgc
    13681 caagcgttcc gcagattcgc tgcgcgaagc caaggaaaag gccaacgagg tgatcgccgg
    13741 tgccgacagg cgcggcgctc agctcatcga ggaagccaag gcagccgcca gggtggaagc
    13801 cgagaaggtg attgcctccg ccaaggccga gatcgaccag gaagtcgagc gagcccgtgc
    13861 agccctgcgc gaccgcgttg ccgaactcgc ggtcgccgga gccgagcgaa tcctcaagcg
    13921 tgaagttgac gccaaggcgc acggcgaaat gctggccgcg ctgaagcagg agctctagcg
    13981 ccatggctga actcgtcacc atcgctcgcc cttacgccga ggcggctttc cgtctggcgc
    14041 aagagaagaa ggccttggct gcgtggtcgc aacagcttga actgctgtcg gcgattgccg
    14101 ccgacgcgca gatgaagagc tgcatcggca acccacagtt gtcggcaccg cagaagagcg
    14161 agctgttccg ctcgctggcc ggcgacaaga tggatggcga cgcggccaga ctcgtcgagg
    14221 ttctggcgaa gaacgaacgt cttgccgtgc tgccgcagat tgccgagctc ttcgaatcgc
    14281 ttaagggcga agccgaaggc gttcaggacg cagtgatcca cactgcgttc ccgctttccg
    14341 acgcgcagca gaaggccatc gtcgccgacc tcgaacaacg ctacaaaacg cgccttaacg
    14401 cccaagtcgt cgtcgatcag accctgatcg gcggcgtcaa gatcgtcatc ggcgaccaag
    14461 tactcgacac ctccgtccgc ggcaaactcg acgccatggc gaccgcgctg aagaactagg
    14521 agaaattcca tgcagttgaa tccttccgaa attagcgaac tgatcaagag caagattcag
    14581 gatctgaaga tcagcgctga cctgcggacg cagggcaccg ttgtttcggt gaccgacggc
    14641 atctgccgcg tccatggtct aaccgacgtc atgcagggcg aaatgctcga gttcccgggc
    14701 aacaccttcg gcatggcgct caacctcgag cgcgactccg tcggcgccgt ggttctcggt
    14761 gagtacgagc acatctccga gggcgacacg gtcaagacca ccggccgcat tctcgaagtt
    14821 ccggtcggcc ccgagctgct cggccgcgtc gtgaactcgc ttggccagcc gatcgacggc
    14881 aagggcccga tcggcaacaa gatgaccgac aagatcgaaa aggtcgcgcc gggcgtgatc
    14941 tggcgcaagt cggtgtcgca gccggtgcag tcgggcctga aggcgatcga tgcgatggtt
    15001 ccggtcggtc gtggccagcg cgagctgatc atcggcgacc gccagaccgg caagaccgcg
    15061 gtggccgtcg acacgatcat caaccagaag ggtcaggaca tgatctgtat ctacgtggcg
    15121 gtcggccaga aggcttcgac ggtcgccaac gtagtacgga aactggaaga gaacggcgcg
    15181 atggagtaca ccatcgtcgt tgccgcgacc gcctccgaat cggctgcgct gcagtacatc
    15241 gcgccctact cgggatgcac gatgggcgag tacttccgcg atcgtggcca agacgcgctg
    15301 atcatttatg acgatctgac caagcaggcc tgggcctacc gtcaggtatc cctgctgctg
    15361 cgccgcccgc cgggccgcga agcctaccct ggagacgtct tctacctgca ctcgcgtctg
    15421 ctcgagcgtg ccgcccgcgt ttcggaagaa tgggtcgaga agttcaccaa cggggaagtc
    15481 aagggcaaga ccggttcgct gaccgcgctg ccggtgatcg aaacgcaggc cggcgacgtc
    15541 tccgccttcg ttccgaccaa cgtgatttcg attaccgacg gccagatctt cctcgagact
    15601 gacctcttca acgccggtgt ccgtcccgcc atgaacgccg gtatctcggt gtcgcgagtc
    15661 ggtggcgctg cccagaccaa ggtcatcaag aagctcggcg gcggtgttcg tctggcactc
    15721 gcgcagtacc gcgaactcgc ggcctttgcg cagttcgcct cggacctcga cgaagcgacg
    15781 cgcaagcagc tcgagcgcgg tcgcctggtt accgaactga tgaagcagcc gcagtactcg
    15841 ccgatgtcgg tggcgatgat ggcggtcacg ctgctggcgg ttaacaaggg cctgttcgac
    15901 gacgtcgagg tgaagagcgc acttgaggtc gagaagcaga tgcagtcgta cctcaagcag
    15961 aagcacgccg acctgatctc gacgatcgag accaagaagg atctcgacgc cgacgccgag
    16021 cagaaactca ccaaggccat cgaagagttc aaggccaccc tggcctgatc gaactagacc
    16081 taggagagcg ccatgcccag cggcaaggaa attcgaacca agatcaagag cgtcgaaaac
    16141 acgcgcaaga tcaccaaggc catggagatg gtggccgcat ccaagatgcg caaagcgcag
    16201 gagcggatgc gtgcctcacg tccctacggc gaaaagatcc ggcgtgttgc ggctcacctg
    16261 tcgcacgcgc tgaccgagta tcgccatccg ttcctcgtgg tccgcgagcc gatcaagcgc
    16321 atcgggatga tcaccgtcac ttccgacaag ggcttgtgcg gcggcctgaa cagcaacgtg
    16381 ctgcgcctct cgctggcccg cttcaaggaa tggcagggtg aaggcaagca ggtccaggtc
    16441 accgcgatcg gcaacaaggg ctttggcttc atgcaacgcc tgggggccga ggttgtctcg
    16501 cacgttaccg ggcttggcga tacaccgcac ctcgagaagc tgatcggacc ggtcaaggtc
    16561 cagctcgacg cctacgtgcg cggcgacatc gacgcgctgt tcatcgtcta cacccgcttc
    16621 atcaacacga tgaagcagga gccggtgatc gagcagctgc tgcccctgtc cgacgatgtc
    16681 gtcggcagcg agtcggcgaa gtgggactac ctctacgagc cggatgccaa gtccgttatc
    16741 gacgatctgc tcacccgcta cgtcgaagcg ctcatctacc agtcggttgc cgagaacatg
    16801 gcatccgagc aaagcgcgcg gatggtggcg atgaagtcgg cttccgacaa cgcgaaaaac
    16861 gtcatcggcg aactgaagct ggtctataac aaggcccgcc aggctgcgat caccaaggaa
    16921 ctctcggaga tcgtcgctgg tgccgccgcg gtgtaagttt tattgattgg ggatacaacg
    16981 atgagtcaag gaaacattgt tcagtgtatc ggcgccgtgg tcgacgtgca gttcccgcgt
    17041 gacgcaatgc cgaaggttta cgacgcgctg atgctcgaca agagcgaggc atcgtcgatg
    17101 gtcgaggcag acctgacgtt cgaagtccag cagcagctgg gcgacggcgt ggtccgcacg
    17161 attgccatgg gttcaagcga cggcctgcgc cgtggcatga agatcaacaa caccggcaag
    17221 ggcatcgccg tgccggtcgg cgaggcaacg ctcggccgca tcatggacgt gctcggtcgc
    17281 ccgatcgacg aggccggccc aatcggcacc gatgagcgcc gtgaaattca ccagtcggcg
    17341 ccgaagttcg atgagctgtc gccctcggtc gacctcctcg aaaccggcat caaggtgatc
    17401 gatctggtct gcccgtttgc caagggcggc aaggtcggcc tgttcggcgg tgctggcgtc
    17461 ggcaagaccg tcaacatgat ggagttgatc aacaacatcg ccaagcagca ctcgggtctg
    17521 tcggtattcg ccggtgtcgg cgagcgtacc cgcgagggca acgacttcta ccacgagatg
    17581 aaggactcca acgttctcga caaggtgtcg atggtcttcg gccagatgaa cgagccgccg
    17641 ggcaaccgcc tgcgcgtcgc gctgaccggc ctgaccatgg ccgagcgttt ccgtgacgac
    17701 ggccgtgaca ttcttttctt cgtcgacaac atctatcgct acacgctggc cggtaccgaa
    17761 gtttcggcgc tgctcggccg gatgccgtcg gccgtgggct accagccgac gcttgccgaa
    17821 gaaatgggcc gcctgcagga gcgcattacc tcgaccaagg ttggctcgat cacctcgatc
    17881 caggccgtct acgttcccgc ggatgacctg accgacccgt caccggcgac gaccttcctc
    17941 cacctcgact cgaccgtcgt gctgtcgcgc gacatcgccg ccctgggtat ctacccggcc
    18001 gtcgatccgc tcgattcgac ctcgcgccag ctcgatcccc tcgtcgtcgg ccaggagcac
    18061 tacggcgtcg cgcgcgccgt gcagggcacg ctgcagcgct acaaggagct gcgcgacatc
    18121 atcgcgattc tgggcatgga cgaactgtcg cccgaagaca agctggccgt ggcgcgcgcc
    18181 cggaagattc agcgcttcct gtcgcagccc ttccacgtcg ccgaggtctt caccggctcg
    18241 ccgggcaagt acgtgacgct caaggacacg atcaagggtt tcaagatgat cgtcgaaggc
    18301 gaatgcgatt ccttgcccga gcaggcgttc tacatggtcg gcggcatcga ggaagcattc
    18361 gagaaggcca agacgctgca gtaatcgaca tccggcagac cggtgccctc tgcgggttcc
    18421 ggtcgccggc caggagggag caccatggca ttgagagtac atctggacgt cgtcagcgcc
    18481 gaagagtcga tcttctcggg cgagaccgac ttcgccgtgt ttccgggcga agccggggag
    18541 cttggtatct acccacggca caccccgctg cttacgcgca tccgcccggg gacgatccgc
    18601 atcaagaacc cgtcgagcaa cgattacgag ctggtctatg tttccggcgg catgctggaa
    18661 gtgcagcctg acatgatcac cgtgcttgcc gacaccgccg tgcgcgccgc cgacctcgac
    18721 gaagcgaagg cgctcgaagc caagcgccag gccgaagagg cgctgcgcaa ccgttcttcg
    18781 gaaatcgact acgcgaccgc cgaggccgaa ttggccgaag ccatcgcgca gctgcaggca
    18841 atcgaaaagc tcaagcggcg tggccactaa gctttgacga ttctgtgaaa aggcagcctc
    18901 cgggctgcct tttcttttcc ctgaacctcg ggcgtcacgc ccgcaggaac cggaacgcgg
    18961 aggcaattga gcgccatgac gcaatgcgac taagatcatc ggactcgtgc cgtcccggtg
    19021 caaccgaaag cccgcttgcc acacgctgcc gaagccgtcc tctacgtccg cctgccctgc
    19081 tggaagatct atccgggtgg cgtggtgtac gtagccgatt acgttcacaa gaaccgcccg
    19141 ttggtccgcc agcacctgct cgatctcgcg ttggtgccgc gccggaagcg ccgtgccgcg
    19201 ttgcacgaat gcatcgacag ctttcatccc gacatcgtcg ccttctcctg gcggaacatg
    19261 cagtccttcg ggccgcaccc cgaagacgac gcactcgatg tcgtaatgaa tttcgaccac
    19321 gcacgtaatc cgctgcgccg cctgcgcgcc gccggcgacg cgttgcgcat catttacgac
    19381 tacacggcag cccgcctcga caacttcgcc ttcatgcggc tggtccgccg ccggttgcct
    19441 caggcgcgga tcgtcgtcgg cggcaccgcc gtttcgatct tcggacctta cgttgccgag
    19501 aaaatgccga gcaactcggt ggtcgtcgtc ggcgagggtg aagacagcat gctgtctatc
    19561 gtcgatggcg tcgaagtgcc cctcggcgag acctaccgga aggacgccga gggacgcatt
    19621 acccaccacg agcgcgtcgc caatttcgag ttagcggaac tcggcgccgt cgatttcgat
    19681 tacatcacaa cgatcttccc tggtttcggt gcctacctcg acgatgacgc ggtcggggtc
    19741 aacaccaagc gcggttgccc cttccagtgc catttctgtc tgtacaaccg gattgagggg
    19801 ccgaaccagc ggtatcgcga tcctgccgag atcgtcgatg aaatcgaggc cctcaaccgc
    19861 cagcatggcg tgcggcgcat ctggttcacc gacgcgcagt tctgttcgac caagcgcagt
    19921 accgaacacg tcgagcaggt gctggacgaa ctgctcgaac ggaagatcaa aattggttgg
    19981 tcgggctatc ttcgcctgaa tcacctgaca ccgtcgatcg cccggaagat gatcgcaagc
    20041 ggaatcgaga gcatcgacct gtcctttgcc gggacgcagg aagtgatcga cagcctgacc
    20101 ttgggctacc gcctcgacca acagatggac gccttccgca tgattcgcga gagcggttac
    20161 ggcgagcaga agatcaagct ctacatgccc ctcaacgcgc cgggcgaaac cgccgaaacg
    20221 ttgcggcgga ccattgccaa ggtgcaggag ctctacgcac tgttcgggcg cgacaacgtc
    20281 ctgcccttca tcttcttcat cggtgtccag cctggaacgc cagtcgagaa acggctgatc
    20341 gacggcggct acctgccggc cgactacgat ccactgacgc tcaatccgtt cgtgatcaag
    20401 aagctgctct acaacccgcg accgctggga acgctaatcg ggcgggccta tctggaggcc
    20461 gtcgattcgc tgaaccggaa ccaggacgag tacatcggcc gcgcgacgct cgacgtgctc
    20521 gcccgtgagc ttggcggcgc ctgatcaagg ctcaccggcg agcgctcagc gaccgccgct
    20581 ttgggcgagt cggcctaccc gacggtcgag cgactcgacg gcatcgaaca gttgacgaag
    20641 ctcggcgtca aaccctgctg cctcggcgtg ccgcagtagt ggcagcgaat ccgctgcagc
    20701 acgatcgcgg agcgccgcct cggcgcgcga caatgcatcg gcaccgactg cgccgagggc
    20761 cttcagccct tcaccaatgc gattggcgac gatgtcgccg gtaaatggcg ccagccagtc
    20821 ggccgggtcg aagcgaagcg tccgcgccgc tgccgagaag tcgtcggcga gatcggcagc
    20881 cccttcaatg cgggcgccgc gtagcgggtc gccggcggga agcgcagtca gggattcggc
    20941 ggaaatcgtt acccgtaggt cggcagccgg atcgccgtgc agatcgacga tacgcagcgc
    21001 accgccttcg tctatggcca aggtagcggc gccgcccagc acgccgacac gcagccgtcg
    21061 cccggtgtgc cgcgcgagcc gctgtcgggc ttcggcctga tcagcgagca ggcggtcgag
    21121 caggctttgc agcagcgcga tcaccgcagg ccgccgttca gaagcggaag ccgcgatgca
    21181 gcgcgacgac gcccgcggtg aggttgaagt aatcgactcg ttcaaagccg acggcctcca
    21241 tcatcgcctt caaggattcc tggtcggggt gcatgcgaat cgactcggcg aggtaacggt
    21301 agctctccga gtccttggcg accaaatcgc cgagtcgcgg cagcacctga aaggaataga
    21361 aatcgtaggc cggtgccagt ggtttgcaga ccttcgagaa ctcgagaacg agcagacggc
    21421 caccgggctt gagcacgcga tacatctcgg ccagtgccaa gtccttgtgc gtcatgttgc
    21481 gcagaccgaa agcaaccgtg acgcagtcga aatagcccga cgcaaagggc agcttctcgg
    21541 cgtcgcactg cgcaaccggc agcagcacgc ccgaatcgac gagccggtcg cggccgcagg
    21601 agagcatcgc gtggttgatg tcggtcagcc aaacttcgcc ccgggttcca actcgctcgg
    21661 caaaggcctg cgccagatca ccggttccgc cggcgatgtc gaggactcgc tggccggccc
    21721 ggacaccgct ctgtccgatc gcgaagcgct tccacagtcg gtgcagcccc gccgacatca
    21781 ggtcgttcat caggtcgtaa cgggtcgcca ccgaatcgaa gacttcgcga acatgcgccg
    21841 ccttcgcgct ctcgtcgacg gtccgataac cgaaatgggt cgtctgttcg ctcaccgcag
    21901 caccccgttc aatgactgcc gcaaccgccc gagccgcagg agccgctgcc gcaaccgccg
    21961 gctgccttgg ggcttacgtt cgctccggct tcgcggctgg cgcccgcggc ttcgacctgt
    22021 tcgaggtagt tcttccagag ctcatcctga tgcgacgcga ggttgaagag cagatcccac
    22081 gagtaaattc cgctgtcgtg gccgtcggaa aaatgtgggc gcagcgcgta attgccaacc
    22141 gcttcgagat catcgatggt cacctcgcgc ttgccaacct gcagggtctc ctgccccggc
    22201 ccatggcctc tcgcctcggc cgacggggtg tagacccgaa gatactcgaa aggcagtgag
    22261 aagcgtttgc cgtcgggata gaccagatcg aggcagcgcg aatgcgtcct cagcagaact
    22321 tcttcgggaa ttggtgggtt gttttccagg ccagccatga gaggggctcc tttcgcgcac
    22381 agggcagcag tttatcagcg agcgtttgct gagtccttta ggccatgcca accccgaatt
    22441 ggttcggccc aactaccggt tgcccccaac cgggtagatg cacgcagtgc gaaagacatt
    22501 ttggcccttg gccgtggccg ttttggagaa acgcccgtag tggcaacctt cggcttaatt
    22561 aaaaatcttt gggagaatct ccgatgtcga tgtgccgccg cagtctgctt ctccgtttcg
    22621 tcgttcgccg gcgccgccga gaatccggcc tcgctacccg gcagcgccat caaataagcc
    22681 gctgagcaat gtcatgcggg gccgcgcacc gcgcgcggcc cttttcgctt tggcaagtcc
    22741 aacccccgta gtacgaagga agaacaatga ccctgcgcgc ccgcttgatc ctgatggccg
    22801 ccgtcgccaa gttccgcgtc gcctgaggct tactgcgcgg tcagtctgtc gagacgaccg
    22861 ctgagggctg cggcgagggc cgcggcatcg accgggcggg ctgcggcctt gcgcatcggc
    22921 tcgccccaga ttggattggg aaaatgccgg tcgtcacgcc agcgcgggat cacgtgccag
    22981 tgcaggtgcg gcacgacgtt gccgaggctc gccaggttga tcttgtccgg ttgcagcgat
    23041 tcgcgcagca ctgattcgac ggcccacacc actgccatca gcgcgtcgcg ctcagcagcc
    23101 tggaggtcgg tcatctcgct ctggtggcga ttgaggatga cgcggacgaa gcccggatag
    23161 tcggcatcgt cgacggcaac gacgcggcac aaggcgttct gccagaccag cgcaccgccg
    23221 gccgcgacgc acagcgggca gtcgctgtcg gcggtaacct tgctcagacc agcacccgtt
    23281 cgatgccgcc ggcgttggcc ttgtcgacgt agtcgaccag ccagtcatca ccgagcagcc
    23341 gtttcgccag ttcgacgacg atgtagtcgg gttcgaccgc cgcatcgccg acgtagcgat
    23401 gcagcccctg aaggcacgag gggcacgagg tgagcagctt gaccggcccg gcgaagccgt
    23461 cgctgcgcag tttggcatcg cccttctcga tctcctcctg cttgcggaag cgtacctgcg
    23521 tcgagacatc gggccgggtt actgccagcg tgcccgattc gccgcagcag cgatcggaaa
    23581 gcacgacggg ctgcccgagc agcgtgttgg cgaccgtgag cggcgcgtgc gttttcatcg
    23641 gcgtgtggca ggggtcgtgg tacaggtaac gcacgccgtc gacgccgtct agcttgacgc
    23701 ccttctccat caggtattcg tggatgtcga gcaggcggca acccgggaaa atcttttcga
    23761 actcgtactt ctgcagctga tccatgcagg tgccgcagga gacgatcacg gtcttgatgt
    23821 cgaggtagtt gagcgtgttg gcgacgcggt ggaagagcac gcggttgtcg gtggtgatct
    23881 tgcgtccctt cacctcctcg cctgctgatg tctgcgggta gccgcagcac agatatccgg
    23941 gcggcagcac ggtgacggcg ccaacttcgt agagcatcgc ttgcgtcgct agcccgacct
    24001 gcgagaagag ccgctccgag ccgcaaccgg ggaaatagaa caccgcctcg gcatcgtcgc
    24061 cggccttctt cggatcgcgg atcaccggaa cgattcggtc atcctcgata tcgagcagcg
    24121 cccgcgaggt gcgtttgggc aggccacccg gcatcggctt gttaaggaag tgaatgatct
    24181 gcgccttgag cggcggtcgg ccgagcgtcg ccgccggtcg tttgaccgag ggctcgatca
    24241 gcccgaagga cttcgccagc cgatggccca ggcgctgggc ctgatagccc cattcgatca
    24301 tgaccttgcg gatcagcttg atcgtcgccg gatccttgac cgtcaggaag gccatcgccg
    24361 cagccgtgcc ggggttccac tttttcttgc catgttcgcg cagaaagttg cgcatcgcca
    24421 ccgagacatc gccgaagtcg atgtcgaccg ggcagggatt caggcacttg tggcagaccg
    24481 tgcagtggtc ggcgacgtcg gagaactcgt cgaagtgctg cagcgagacg ccgcgccggg
    24541 tctgctcttc gtaaaggaag gcctcggtca gcagcgaggt cgctaggatc ttgttgcgcg
    24601 ggctgtagag caggttggcg cgcggcacgt gcgtcgaaca caccggcttg cacttgccgc
    24661 agcgcaggca gtccttgatc gagtcggaga tccgcccgat ctccgactgt tccatgatta
    24721 gcgattcgac gccgagcagc gcgaacgacg gcgtgtaggc gtttgcgagg tcgccgcctt
    24781 cgagaagctt gcccttgttg aagcggccct ccgggtcgat ctttgccttg taggcgcgga
    24841 agggcgcgat ttcgttctcg gcgaggaact cgagcttggt gatgccgatg ccgtgctcgc
    24901 cggatatgac cccgtcgagc gagcgcgcca gcgccatgat tcgggcgacg gcggtgttgg
    24961 cggtctgcag catcgcatag ttgtcggagt tgaccgggat gttggtatgc acgttgccgt
    25021 cgccggcgtg catatgcagc gcgacaaaga cgcggccgcg cagaacctcc tggtggattg
    25081 cgtcaatgcg ctccaaggtc ggacggaaga agacgccgtc gaagatttcc ttgagctgcg
    25141 gcttcagctc cttcttccag gagacgcgga ccgagtagtc ctgcaggcgg tggaagagcc
    25201 gcggattcgc ggcccggttc gagagctctg cggcctctat accaaactcc gagaaacggg
    25261 cctcggcctc ggcgagcggc aggtcaaggc tatcgagcag ccactgccag cgcgctcgcg
    25321 ccgcgccaac cgcgtcgagt gcggtcgtgc gccggtcgcc gatcaaagcc gcgcggtcga
    25381 cgccggcgtc cccgaggtgt agtggcagct caccgtcaag aaactcggca agcgcgtcgc
    25441 agagcgcgat cttgttggcg atcgacagtt cgatgttgat ccgttcgatg ccgtcgcagt
    25501 agtcgcccat gcgcggcagc gggatgacca cgtcttcgtt gatcttgaag gcattggtgt
    25561 gccgggagat cgccgcggta cgcgagcggt cgagccagaa gcgcttgcgc tgctcgggcg
    25621 ttgtggcgac gaagccctcg gcgcctcgcg cgttgcacat ccgtaccacc tcggaggcag
    25681 cgcgcatcac cgagtcctcg tctgcaccga cgatgtcgcc gatcaagacc atcttcggcc
    25741 ggccgtggcg cttggccttg gtcgtgtagc cgaccgcctt gacgtagcgc tcgtcgaggt
    25801 gctcgaggcc ggcaagctgg acgccggccg cgtgcgcggc gccgcccggc ttgaagtggt
    25861 cggtgatctc gacgatcgcc ggcacggcct cgcgcacctg gccgaagaac tcgaggcaca
    25921 cggtacgcaa cacagggggc atcctgtgca atacccagcg cgcggcaacg atgatgccgt
    25981 cgcagccctc cttctgcacg ccgggcaggc cgccgagaaa cttgtcggtg acgtccttgc
    26041 ccagcccggt cttgcggcag gcggcgccag gcatcgtaag gatttcaccg gcgaggcgtt
    26101 tcttgcccgt gctgtcgtag cgcgtgacgc ggaactcgac ggtttcctgc tcgtggatct
    26161 tgccgaagtt gtgcttgagc cgttcgacct ctagccagtc gccgtcgggc gtcaccatct
    26221 tccaccaggc caggttgtcg agtgccgtac cccagagcac ggccttcttg ccgcccgcat
    26281 tcatggcgat gttaccgccg atgcaggagg ccgacgccga ggtcgggtcg acggcgaaga
    26341 cacggccggc gcccgatgcc gcttcggcga cgcgctcggt gatgacgccg gccgaggtgc
    26401 ggatcgtcgc gtagggtgtc tcgcagccgg gcagcgtcaa ttcctcgacc gggccgatgt
    26461 cgatcagctt ctcggtgttg atcaccgccg aaaacggcgt cagcggtacc gcgccgccgg
    26521 tgtatccagt accgccgccg cgcggaataa tggtcaggcc gagcgaaatc aggtcgcgga
    26581 ccagcggccc gatttcctct tcggtatcgg gcgtgagcac gacgaagggg tgctcgacgc
    26641 gccagtcggt cgcgtcagtg acgtgcgaaa cgcgcgcaag gccgtcgaag cagatgttgt
    26701 cgcgccgcgt gtgccgcgac agcacgcgca gcgtccgccg gcgcagcgtt tcggtggtct
    26761 cgaaccagcg ctcgaaatcg cgcacggcat tgcgcgccgc ggccagcagg ctggcgacgc
    26821 gctcgttgcc ggcgcgccgc ttgtcgactt cgttgaggcg atgatgcagc gccccgatca
    26881 gcgcatcgcg gcgcttctcg ttggcgagca ggtcgtcttc caggtacggg ttgcggcgga
    26941 ccacccagat gtcgccgagc acctcgtaga gcatccgcgc cgagcggccg gtgacgcgtt
    27001 cgcgccgcag ctcgtcgacg atcgaccaag cgtcggcacc gagcaggcga atgacgatct
    27061 cgcggtcgga gaacgaggtg tagttgtagg ggatttctcg caggcgggca gtcatcgaag
    27121 gttcgcagac tcaggagtac cagatcggcc gggcattatc gcaccgcagc aaggcagcgt
    27181 tctaccgcgt cgacgacagg ggattgcctc gtccccgctg gccggggcag ttcgataatt
    27241 gtttcagccg gaccgccatg ccgccatggc gcgattcgaa agggtgaagt gcggatcaaa
    27301 ccaagcccac aggtaggcac cgatagctcg gcaatgacca agatcaacag cgctttgggt
    27361 ggtgtagtta tgcttggaac agtcaatgtc ggtattcggc catgcgctcg cccactctga
    27421 tccataagtt cctgcggatg tttcttccaa tggtcgcgct ggccgtggca gtagtcctcg
    27481 tctatggaca aggagagttg gagcgacaga tcagccgact gcgcaaccac gatgctctcg
    27541 tcatcggtct gggcgccggt gcgctcgcgg gccggatcga gacgatccac cgcgaccttg
    27601 ctttcctctc ggcgagccac agtctgagag aggcgataaa cagaccgtcg gcacagcatc
    27661 tcgcagaatt ggccgaggat ttcgtcattt tttcccgcag caagggcgtc tatgaccaga
    27721 ttcgctggat cgatgagacg ggattcgagc gggtgcgagt ggagttgacg aacggccagc
    27781 ctgagctggt tccgcaagaa cgacttcagg acaagcggca gcgctactac tttgtcgcca
    27841 ccatggcgtt acgccccggg gaaatcttcg cgtcgccgct cgacctcaat gtcgagcaga
    27901 accagatcga ggtcccctac aaaccgacgt tgcgattggc aactccggtc gccgacgccc
    27961 aagggcgact gcgcggcatt gtcatcgtga attacctcgg tagggacttg ctggaaacct
    28021 tcgatcgaga gactaaagcg ctcgctggac gatcgatgtt ggtgaatcag gatggttact
    28081 ggctaaaggg tacaacccca gacgatgaat gggggttcat gttcgatcgt tcagagttga
    28141 gtctggcggc gcggtatcca cgtgcgtggc aacacatcag cagtgtcgac cggggccagg
    28201 tcgccctcga ggatggcatc tggtcttggc aaacagttcg cccgttttcc agtcgtggct
    28261 tgaccagcgt tgagacaatc acggcgcagc agcatgtctg gaaagcggta tcccatttgc
    28321 acgactcgga cctgatgggg atggcgcgta cagtctggtt gagattgtct gccgtgatgc
    28381 tgctcttggc tgccggcgtg gcgtatggct gctggaagct agttctggcc tgggatgctc
    28441 gcgctgaggc cgacaatcgc ctcaggagcc tcaatgtcga ccttgaggcc acggtggagt
    28501 cacgcaccga ggcgttgcgg cttagcgagc ggcgtttccg cgatgttgcg catatctccg
    28561 ctgactggat ctgggaggtg gatgccgagg cccgctatgt ttacgcctcg gagagcgtcg
    28621 aagagcttct cggctaccga cccgaagaga tcatcggcaa gacagcgttc gacctgatgc
    28681 cgccggacga agcgcggcgg gtaagggtcg cgttcgccga gatcgcagaa gctcgccggc
    28741 cgtttcgcga ccttgaaaac atcgtgttgg gaaaggatgg taaggcccac atcacgctga
    28801 ccaatggcat gccgattctt ggcgccgatg gagcgctgct cggctaccgg ggcgtagacc
    28861 gagacatcac ggcccagcgg caaatggaac aacagatcag gcaaatggcc ttcatcgacg
    28921 gcctgaccgg gctgcccaac cgccgtctgt tcgccgaccg cctcgaaatt gcgctggcgg
    28981 caagccggcg cagcggcgaa cgcggagcgc tgttattcct cgacctcgac aagttcaagg
    29041 cgcttaacga tcgcgccggc catgaagctg gagacctcct gctggtcgaa gctgcgcgcc
    29101 gcctgcaggg ctgcgtgcgc gaaatggata ccgtggcgcg tctcggcggc gacgagttcc
    29161 tgatcatcct cggtggcctg gacgctgacg ataagcgagc ggccgaacag gtcaagcatg
    29221 tcgccgaaaa gatcgccgcc gcaatgggcg caccctatct gctcaagttg gcggacaaaa
    29281 cggtcgaaca ccactgcacc gcgagcatcg gtgcaacgct gtttctcggt cacgaagtgg
    29341 acggcgagga actcttgcgc cgcgccgatg cggcgatgta cgaggccaag gcggcagggc
    29401 gagccgcaat acgcttctcc aagcacagcc cctgaatgga gaaacggcct agcctgcgtg
    29461 ctggtgagcg ggatggcgcc ctcaccgccg aagttgggcg agcgctgtag ccgaacccgc
    29521 cagcgatcac atcgacggcg tcacaacgca gcggcgccct gagccagcaa ccagtagcgc
    29581 gcccccttac caaccgccat aaacgatatc gccgccagcc aatgtgtgcg cagccagccg
    29641 gcggcgacac aaagcaggtc gccgatcagc ggcgcccagg cgagcagaag cgacgcactg
    29701 ccgtaacggc gtacgaggtc catatgcggc acgcgctcga gctgctgcca tttcggaagg
    29761 aagcgaccgc aagcgtacga ggtcatgccg cctagcgtgt tgccgacggt cgccagcacc
    29821 agcgcgtcgg ccacgcggtc ggggtggcct ttgacgaagg catacagcgc aatctcggag
    29881 ccgccgggca gcagcgtcgc cgccagaaag gacagcgcga aaagtccggc gagcgaggtt
    29941 tcggcgtcta agaagtcgct cacgtcgcgg cgacggcgcg ggccaggtcg ttcgccagcg
    30001 cgtcgaccgc ttcgtcctcg gtggcccacg agcacatgaa gcgcgaggcg ccgccgatga
    30061 agtcgtggaa catccagccg gcgtgccgca gtctttcgcc ggcaccggcc ggcagctcgg
    30121 cgaagacgcc gttggcctgt actggatgcg cgagccgcac gccggggatg gcggcaaacc
    30181 gtgcggccaa ggcctgcgcc tgcgcgttgg cgtgcgcggc gcggtggagc caggtgtcgc
    30241 cgtcgagcat cgccagccag ggcgccgaga gcacgcgcat cttcgaggcg agctggccgg
    30301 cctgcttgca gcggtaggcg aattcctcgc cgagacggcg atcgaagaag accacggcct
    30361 cgccgatcgg caggcccatc ttggtgccgc caaagcacag cacgtcgacg ccggcgcgcc
    30421 acgtgagatc ggccggcgag acgccgaggc cggcgacggc gttggcgaaa cgggcgccgt
    30481 ccatgtgtac gtgcaggccg tcggcgcgcg cgactgcggc gattgcggcg acttgggcgg
    30541 ggctgtagac ggtgccgaac tcggtggcct gcgtcaggct gacggcgcgc ggccgcggaa
    30601 agtggatgtc gctgcgcttc ttcaccagcg cggccaccga ctcgggagaa atctggccct
    30661 cgcgttgcgg cgcggtcagc agcttgctgc cattggagaa gaattcgggc gcgccgcact
    30721 cgtcggtctc gacgtgcgcg ctctcgctgc agatcacgcc gtggtagctc tggcaaagcg
    30781 aggcgagtgc cagcgaattg gcggcggtgc cgttgaagac gaagtaaatg tcgcagtcgg
    30841 tctcgaagac tgtgcgcagg cggtcacaga cctgatgcgt ccacgcgtcg ctgccgtagg
    30901 aagcgacgaa accggcgttg gcggccgcga gtgcggcaag cgcttcggga caggcgccag
    30961 cggtgttgtc gctggcgaac tgcagtttcc agccgggagt ttcggcagcg ggcatggcga
    31021 atcgccttgt aaagcggagt cgggcgcgga ttgtagcaag cacacgtgcc ataatcgccc
    31081 gctcgattcg cccgtgaaat catcaagatg aatgctccag cgccggtcct taacgattac
    31141 ctcgaacgca ttctcaatgc gcgcgtctac gacgtcgccg tcgagacgcc gctcgatccg
    31201 gcgcccggcc tctcggagcg gaccggcaat acggtcctgc tcaagcgcga agacatgcag
    31261 ccggtgttct cgttcaagct gcgcgg
//
LOCUS       DATDAA010000446         2889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_102464,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000446 DATDAA010000000
VERSION     DATDAA010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102464"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VIS73_13380"
     CDS             complement(<1..340)
                     /locus_tag="VIS73_13380"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5764194.1"
                     /translation="MTFGPEVVAERLDALETADNKPDRYVIALSGGLDSTVLAHALTV
                     TRERHGKSLLAVHVDHQLHPESGHWNDYCRRLADTLDLEFVAETVSVVADGGGLEAAA
                     RKARYAALARH"
     gene            complement(337..1296)
                     /locus_tag="VIS73_13385"
     CDS             complement(337..1296)
                     /locus_tag="VIS73_13385"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY5764195.1"
                     /translation="MDLKFLDFEQPIAELEAKIEELRFVGDDSEININEEVARLRSKS
                     EALTKSIFSKLSAWQIAQVARHPMRPYTADYLEVLSPDFQELHGDRMYADDPAIIGGV
                     GRIDGRAVMFIGHQKGRDTKERVRRNYGMPKPEGYRKAQRLMRLAEKFSLPVVTFIDT
                     PGAYPGVGAEERGQSEAIAFSLYLMAGLKTPIISVVIGEGGSGGALAIGVADKLLMLQ
                     YGIYSVISPEGCASILWKSADKAEDAAEAMRITASSLNNFGLVDEVLREPLGGAHRNP
                     AEAAEVIRNALIKALDELDSVPIDQLLENRQRRLAGFGEFKEA"
     gene            complement(1351..>2889)
                     /locus_tag="VIS73_13390"
     CDS             complement(1351..>2889)
                     /locus_tag="VIS73_13390"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY5764196.1"
                     /translation="NQAEIDYLHPDLKPVLEETYGVILYQEQVMQIAQVLAGYTLGGA
                     DLLRRAMGKKKPEEMAKQREIFMSGATERGVAKPTATRIFDLMEKFAGYGFNKSHSAA
                     YAVLSYQTAYLKAHYPAAFMAAVMSADLDNTDRLVTLKDDCRKLGLELEAPSVNRSSH
                     HFSVAGDRAILYGLGAIKGVGRSAVEALIADREANGAYGSLAEFCRRVDHDKVNRRAL
                     EAMIKSGAMDDFGQSRRALMHQVPEALKSADQQARAAAAGQNDMFGLEAPTEPDDAAV
                     SMDMDEWPERLLLSNEKEALGLYLTGHPFHAVRSDAHCFVDGNLADLAAEPPPQTARG
                     ERDYAQSRREVTIAGLVVDVRKRGNRISVVLDDDSGRMEVSLFGEAFQEFRHLLVKDE
                     IVVVSGPLRYDDFVGAWSVNVKNVLHIDRVIESRAKSMLLRLAPNGQGDQLLMKLHDV
                     LLPYREGNCEVAVQYFGDNAKARLQLGAEWSVRPSRELRDKLSELLGSNNVRLLYAPG
                     REIM"
BASE COUNT          528 a          940 c          827 g          594 t
ORIGIN      
        1 tatgccgggc aagcgccgca tagcgtgcct tgcgcgcggc agcctcgagc ccaccgccgt
       61 ccgcaacgac cgacaccgtc tccgcaacga attcgaggtc gagtgtgtcg gcaagcctgc
      121 ggcagtagtc gttccagtgg ccggactcgg ggtgcagctg atgatccacg tgcactgcca
      181 atagcgactt gccatggcgc tcccgcgtaa ccgtcagcgc atgcgcaagc acggtcgaat
      241 ccagcccgcc acttaatgcg attacgtatc tatcgggttt gttgtcggcg gtctcgagcg
      301 cgtcgagacg ctccgcgacg acctcgggcc cgaacgtcac gcctctttga attcgccgaa
      361 tccggcgagc cgcctctggc ggttctcgag aagctggtca attggaacgg agtcgagttc
      421 gtccagcgcc ttgatcagcg cattgcggat gacttccgcg gcttcggcgg gattacggtg
      481 cgcaccgccc agcggttccc gcaaaacctc gtcgacgagg ccgaagttat tgaggctgga
      541 cgcggtgatg cgcatagctt cggccgcatc ctccgctttg tccgcacttt tccagaggat
      601 ggacgcgcag ccttcgggcg aaatgaccga gtaaatgccg tattgcagca tcagcagttt
      661 gtccgccacg ccgatcgcga gcgcgccgcc ggatccgcct tcaccgatga cgacgctgat
      721 gatcggtgtc ttgaggcccg ccatcagata caggctgaat gcaattgcct cgctctggcc
      781 acgctcttcg gcaccgaccc cgggataggc acccggtgtg tcgatgaacg tcaccacggg
      841 tagcgagaac ttctcggcca gccgcatcag tctttgcgcc ttacggtacc cctccggctt
      901 gggcatgccg taattgcgcc gcaccctttc cttggtatcc cgccccttct ggtgaccgat
      961 aaacatgacg gcgcgtccgt cgatacggcc gacaccgccg atgatggccg ggtcgtccgc
     1021 atacatccga tcgccatgaa gttcctggaa atcgggcgag aggacctcca ggtaatccgc
     1081 cgtgtagggc cgcatgggat gccttgcgac ctgcgcaatc tgccaggcgc ttagcttgga
     1141 aaatatgctc ttcgtcagcg cctcgctctt tgaccgcaag cgcgcgactt cctcgttgat
     1201 attgatttcg gagtcgtcgc caacaaaacg caattcctcg atcttggcct cgagttcggc
     1261 gatcggctgt tcaaaatcca ggaatttcag atccatggcg gagtcttcgc tagcacttcg
     1321 taatagcggc tgcaggccga aaaaaggact ctacatgatt tctcggccgg gtgcgtacag
     1381 cagccggacg ttgttactgc cgaggagttc gctcagcttg tcgcgcagtt cccggcttgg
     1441 gcgcacggac cactcggcgc cgagttggag cctcgctttc gcgttgtcgc cgaagtactg
     1501 cacggcaacc tcgcaattcc cttccctgta aggtaacagc acatcgtgca gcttcatcaa
     1561 tagctgatct ccctgtccgt tcggcgccag ccgcagcaac atgctcttcg cccggctctc
     1621 gatcacacga tcgatgtgca gcacgttctt cacattgacc gaccaggccc cgacgaagtc
     1681 gtcgtagcgc agcggaccgc tcacaacaac gatctcgtcc ttcaccagca ggtgccgaaa
     1741 ctcctggaac gcttcgccga acaggctgac ctccattcgg ccgctgtcgt catccagcac
     1801 cacgctgatg cgattgccgc gtttgcgaac gtccacgacc agaccggcga tagtcacctc
     1861 gcgccgtgac tgtgcgtaat cacgctctcc acgagccgtc tgcgggggcg gctctgccgc
     1921 gagatcggcc agattgccgt caacgaaaca gtgcgcgtcg ctgcgcactg catggaacgg
     1981 gtgtccggtc agataaagac cgagcgcttc cttttcgttg ctcagcagca ggcgctccgg
     2041 ccactcatcc atgtccatgg aaaccgcggc gtcgtccggc tccgtcggcg cctcgaggcc
     2101 gaacatatcg ttctggccgg ccgccgccgc acgcgcttgc tggtcggcac tctttaaagc
     2161 ctccggtacc tgatgcatca gggcgcgacg cgactggccg aaatcgtcca tggccccgga
     2221 cttgatcatt gcttcgagcg cgcgccggtt gaccttgtca tggtcgaccc ggcggcagaa
     2281 ttcagccagg ctgccataag caccgtttgc ttcgcgatcg gcgatcaggg cttccaccgc
     2341 cgatctgccg actcctttta tcgcgccaag accataaaga atcgctcgat cgccggccac
     2401 gctgaaatga tgggacgacc ggttgacgct gggcgcttcg agttccaggc cgagcttgcg
     2461 gcaatcatcc ttgagcgtca ccaggcgatc agtattgtcc aggtccgcgc tcatcacggc
     2521 tgccatgaac gcggccggat agtgcgcttt caggtatgca gtctgatacg acagcacggc
     2581 gtatgcagcg gaatgggact tgttaaagcc ataacccgca aacttctcca tcaggtcaaa
     2641 gatgcgtgtc gcggtcggct tggcgacgcc gcgctcggtc gcacccgaca tgaaaatctc
     2701 gcgctgcttc gccatctcct cgggcttctt cttgcccata gcacgcctca ggagatcggc
     2761 gccgcccagc gtataacccg caagcacctg cgcgatttgc atgacctgtt cctggtagag
     2821 gatcacgccg taggtttctt cgagtaccgg cttgagatcc ggatgcaggt agtcgatctc
     2881 ggcctggtt
//
LOCUS       DATDAA010000447         3405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_137679,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000447 DATDAA010000000
VERSION     DATDAA010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137679"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..636
                     /locus_tag="VIS73_13395"
     CDS             166..636
                     /locus_tag="VIS73_13395"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5764197.1"
                     /translation="MCPAGRSLYRHGSNGHINGRNAVRFEGAKRDCLPCTHRDRCLRT
                     PDKTPTRQVAYFNDNNPTLASRLAALMRVRIDSDDGRQQYGRRFATVEPVFGNLRHNK
                     RLNRFTLRGKDKVNGQWLLFSLVHNIEKLAWTASTSMAGRTTIILAGWGGDDRR"
     gene            complement(645..1463)
                     /gene="istB"
                     /locus_tag="VIS73_13400"
     CDS             complement(645..1463)
                     /gene="istB"
                     /locus_tag="VIS73_13400"
                     /inference="COORDINATES: protein motif:HMM:NF006038.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEY5764198.1"
                     /translation="MARTALMLTELRLPTIGRLWPEFAERADKEGWPASRFLGALLEH
                     ELAERAKRRIERHRHESQLDLAKTLASFAFAEVPMLSKAHVLALASGDAWLEKGANVL
                     LFGPPGVGKTHVACGLGHALIEAGHRVLYMRTSELVQRLQAARQSLQLPQALARLDRY
                     DLLILDDLSYVRKDQAETSVLFELIAERYERRSLLITANQPFSGWNEVFPDPGMTIAA
                     IDRLVHHSTIFELNMVESYRSKQAARQQKTQRANGKAERQRQSSDDNQTQEAQP"
     gene            complement(1481..2956)
                     /gene="istA"
                     /locus_tag="VIS73_13405"
     CDS             complement(1481..2956)
                     /gene="istA"
                     /locus_tag="VIS73_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEY5764199.1"
                     /translation="MNIRRNKTQALAAAKAGISERSARRIDGATELPSQSPRRYWRSR
                     VNPFAQVWDSEVVPLLKQSPTLMAVTLLRKLQDDHPDQFADGLLRTLQRHVRQWRAEA
                     GPPKEVFFPQEHAPGHRGLSDFTTMGELCITIAGLAFVHLLYHFVLAFSRWEHVEVVE
                     GGESFEALSQGLQNALWQAGGTPREHRSDSLSAAFKNLTEEEDFTARYTALLDHYGMV
                     GTRNNRGLSHENGSVESSHRYLKEALEQALLLRGHRDFDDRAAYEAFVRETVMRRNRR
                     NAAAFRVEREQLCPLPVRRTTDFVEDEARVTRCGTFTVRGILYSAPSRLIGHRLKVRL
                     YSDRLEGYLSGARVLTVARGTRAPGHSRGRMLDYRHFIDSLKRKPQAFKGLTFRDALF
                     PRDAYRQTWAQLEARLPQRQACQTLVALLEMAAQDGVEAVLAERLEALLLIGELPDVK
                     RLREEFAPRQVELPQVSVLLPPISRYDTLLPSAHEPPAVAA"
     gene            3199..>3405
                     /locus_tag="VIS73_13410"
     CDS             3199..>3405
                     /locus_tag="VIS73_13410"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5764200.1"
                     /translation="MSSLVRLPRAQKRRLTRLVQRNREARPVRRAQVVLQLACGDGVT
                     EVSEALTVARSTVYRWLEQFMALGE"
BASE COUNT          628 a         1028 c         1130 g          619 t
ORIGIN      
        1 gccctgattg ccgacaacca gatgcgcaag cgcgacgaac gcttcgaaga ccaagtccgg
       61 cacaaggcga aacccgaccc gctctacgac tagtccggca agcccaggag gtcgccatgt
      121 tacaacccag cggacttcac acacgacccg gtcgccggca cctgcgtgtg cccggccggc
      181 cggtcactct atcggcatgg gtcgaacggt catatcaatg gtcgcaacgc cgtgcgcttc
      241 gaaggggcga aacgcgattg cctgccatgc acgcatcggg accgctgtct gcggacaccg
      301 gacaagacac caacccgcca ggtcgcgtac ttcaatgaca acaacccgac cctcgccagc
      361 cgactcgccg cgttgatgcg agtacgtatc gacagtgacg acggtcgcca gcaatacggc
      421 cggcgattcg ccacggtcga gccggtgttc ggcaatctca gacacaacaa gcgactcaac
      481 cggttcaccc tacgcggcaa ggacaaggtc aacggacaat ggttactgtt cagtctcgtg
      541 cacaacatcg agaagctggc ctggacagcg tcaaccagca tggccggtcg aacgacaatc
      601 atcttggccg gttggggcgg tgatgatcgt cgttgattaa aggtttacgg ttgggcctcc
      661 tgagtctggt tgtcgtcgga tgattgtcgc tggcgctcgg ctttgccgtt ggcgcgctgg
      721 gttttctgct gccgggccgc ctgtttgctg cgatagctct caaccatgtt caactcgaag
      781 atcgtcgagt ggtggaccag gcgatcgatg gcggcgatgg tcatgccggg gtcagggaac
      841 acctcgttcc atccggagaa cggctgattg gcggtgatga ggaggctgcg ccgctcgtag
      901 cgctcggcga tgagctcgaa caacacgctg gtctcagcct ggtctttgcg gacgtaggag
      961 aggtcatcaa ggatgagcag gtcgtagcga tcgagccggg ccaaggcctg gggcagttgc
     1021 aggctctggc gtgcggcctg gagacgctgg accaactcgc tggtgcgcat gtagagcacg
     1081 cggtggccgg cctcgatcag ggcatggccg aggccacagg cgacatgcgt cttaccgacc
     1141 cccggcgggc cgaagagtaa gacgttcgcg cccttctcga gccaagcgtc gccgctggcg
     1201 agcgccagga cgtgggcttt ggagagcatc ggcacctcgg cgaacgcgaa gctggcgagg
     1261 gtcttggcga ggtcgagctg tgactcgtgc cgatgccgct cgatgcggcg cttggcgcgc
     1321 tcggcgagct cgtgctcgag caaggcgccg aggaagcgtg acgccggcca gccttccttg
     1381 tcagcgcgct cggcaaactc gggccacaag cgaccgatcg tcggcaaacg gagctcggtc
     1441 aacatcagcg cggtgcgcgc caactccgac gtggcggcac tcatgcggcc accgccggtg
     1501 gttcatgcgc actcggcagc aaggtgtcat agcgactgat gggtgggagc agcacgctga
     1561 cctggggcag ttcgacttgg cgtggcgcaa actcctcgcg caggcgtttg acgtcgggta
     1621 gctcgccgat cagcaagagc gcttcgagtc gctcggcgag caccgcctcg acaccgtcct
     1681 gcgccgccat ctcgagcagc gcgacgagcg tctggcacgc ctgccgctgc ggtagccgcg
     1741 cttcgagctg tgcccaggtc tgacggtagg cgtcgcgcgg gaacagcgca tcgcggaagg
     1801 tcagcccttt gaacgcctgg ggtttgcgct tgaggctgtc gatgaagtgc cggtagtcga
     1861 gcatgcgtcc gcggctgtga cccggggcgc gcgtaccgcg ggccacggtc agcacccgtg
     1921 cgccggagag atagccttcc agacggtcgc tgtacaggcg gacttttaga cggtgtccga
     1981 tcaggcgtga cggtgcactg tagaggatcc cgcgcacggt gaacgtgcca cagcgcgtga
     2041 cgcgggcttc gtcctcgacg aagtcggtgg tccgccgcac gggcaacggg cagagctgct
     2101 cacgctcgac gcggaaggcc gcagcgttgc ggcgattacg tcgcatcacc gtctcacgga
     2161 cgaacgcctc gtaggcggcg cgatcatcga agtcgcggtg gccacgcagc agcagtgcct
     2221 gttcgagcgc ttccttgagg tagcgatggg atgattccac actgccgttc tcgtggctca
     2281 ggccgcgatt gttgcgtgtg ccgaccatgc cgtaatgatc gagcagcgcg gtataacgcg
     2341 cggtgaagtc ttcttcctcg gtcaggttct tgaacgccgc cgagagactg tcgctgcggt
     2401 gttcgcgcgg cgtaccgcca gcctgccaca gcgcgttctg caggccctgg gacaacgctt
     2461 cgaagctctc accgccttcg accacttcga catgctccca gcgtgagaaa gccaggacga
     2521 agtgatacag gaggtgcacg aaggcgaggc ccgcaatcgt gatgcacagc tcgcccatag
     2581 tggtgaagtc agacaagcca cggtggcccg gcgcgtgctc ttgcgggaag aagacttcct
     2641 tcggcggccc cgcctcagca cgccattggc gtacatggcg ctggagcgtg cgcagtaggc
     2701 catcggcaaa ctggtcggga tgatcgtcct gcagcttgcg cagcagtgtg acggccatca
     2761 gcgtcggcga ctgcttcaac agcggcacca cttcgctgtc ccagacttgg gcaaaggggt
     2821 taacgcgtga acgccaatag cggcgcgggc tctgtgacgg tagttcggtc gcgccgtcga
     2881 tgcgacgggc gctgcgttcg ctgatcccgg ctttggccgc ggctagcgct tgggtcttgt
     2941 tcctgcggat gttcatgtag aggcatacct gttgatcggt gatccgggtt cctggcactg
     3001 ggctgagttc tcaaggagat ctgagcccgc catcttgaac cccaccggcc atcagggttg
     3061 tcgctcaacc ggacaggatg attgtcgccc cctaaatagc cgttccgaaa aatcgatcaa
     3121 atagagagaa ctcaggtgac attcggtgat cagaggttgt gtgtagcaat caccaatacc
     3181 ggaagaggtc acctgagcat gagcagttta gtgagattac cccgagcaca gaagcgtcgg
     3241 ttgacgcgcc tggttcaacg caatcgcgaa gcgcggccgg tacggcgtgc tcaggtcgta
     3301 ctgcagttgg cttgcggcga cggcgtgacg gaagtttccg aggcattaac cgttgcccgc
     3361 tcaacggttt accgctggct ggagcagttt atggcgttag gcgaa
//
LOCUS       DATDAA010000448        12492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_32762,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000448 DATDAA010000000
VERSION     DATDAA010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12492
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32762"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VIS73_13415"
     CDS             complement(<1..669)
                     /locus_tag="VIS73_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY5764201.1"
                     /translation="MMNTAADSFVPRIPAPEPTLVDTVRGVRRDIHAHPELAFAELRT
                     ADLVARELRRIGCEVVTGIGETGVVGVLKNGEGPVIALRADMDALPVAEANTFAHRSV
                     HEGKMHACGHDGHTAMLLGAAEQLARHPQFRGTVCLIFQPAEEGRGGAAAMLADGLLE
                     RWPIERFYGLHNWPGLDENHFAILTGPVMAAADEFEIVIDGRGAPGAMPHLGRDPLLA
                     GAALV"
     gene            complement(699..2138)
                     /locus_tag="VIS73_13420"
     CDS             complement(699..2138)
                     /locus_tag="VIS73_13420"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEY5764202.1"
                     /translation="MSSPFRDDALVWFRRDLRVADHATLATAVAEARRVHCVFVFDRD
                     ILDALDDRHDRRLNFIHDALAALDAELAARGGLSSGARTLTVLHGHAPTVIPQLARRL
                     ACSAVYAGRDVEPQALARDAAVSRALEADGRHLVLIKDQVVFEADEVLTAAGRPYTVY
                     TPYRRAWEKRRDAIPAAIVERRPALRPGVLQPAVEELAPLDAYGFAPVALADIGVVAS
                     EAGALAALAAFQPRLPDYAAARDFPAQAATSMLSPHLRFGTLSVRQAVRAALAANSPS
                     WLGELIWRDFFFAILAAFPQSATQNFKRAYDRVVWRDPAGEAAADFAAWKSGTTGYPL
                     VDAGIRELLATGRMHNRVRMVVASFLCKHLGIHWQHGERFFAAHLLDYDLAANAGNWQ
                     WAASTGCDAQPWFRIFNPVTQSQRFDADGAYLRRWVPELAELPAPAIHAPWKLPAAQH
                     PADYPPPVVDHATGRQDALARFAVLRGAT"
     gene            2214..2777
                     /locus_tag="VIS73_13425"
     CDS             2214..2777
                     /locus_tag="VIS73_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY5764203.1"
                     /translation="MPSTESLANQFLIAMPAMADPFFAKSLVFICEHTDNGALGIIVN
                     RPLDMTLAKLFEKVDLPLTQNDFQQRPVYFGGPVQTDRGFVLHRPLGGFQSTLRINDA
                     LGLTSSRDVLQKISVEGDPNDILVTLGYAGWSAGQLENEIAQNAWLTAPADEAIIFAM
                     PPEARMPAAMQGLGISMTHLADTAGHA"
     gene            2782..3231
                     /gene="ruvX"
                     /locus_tag="VIS73_13430"
     CDS             2782..3231
                     /gene="ruvX"
                     /locus_tag="VIS73_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY5764204.1"
                     /translation="MQPERQRLPSTTILAFDFGERRIGVAVGETETAQAHALTTIEAE
                     ANIDRFAEIAALIDEWRPAMLVVGLPLHVDGTPHAMTARCKRFANQLHGRFGLPVATA
                     DERLTSHDAEALLHEVGRSTREQRAVIDSLAAQLILQGYLNDQQAPA"
     gene            3209..3718
                     /gene="pyrR"
                     /locus_tag="VIS73_13435"
     CDS             3209..3718
                     /gene="pyrR"
                     /locus_tag="VIS73_13435"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEY5764205.1"
                     /translation="MTNKPLPDAERQCAELAAAIRPRLRPDSALIGIYSGGAWIADRL
                     AELLSVSDVGQIDVSFYRDDFEKIGLHPNVRPTAIPFEVSERHLVLVDDVLQSGRTTR
                     AALNVLFDFGRPACVDLAVLADRGGRHLPVAPTWCAWAAELPTSEILILDRGDDGRLA
                     WRTEARDAR"
     gene            3708..4670
                     /locus_tag="VIS73_13440"
     CDS             3708..4670
                     /locus_tag="VIS73_13440"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY5764206.1"
                     /translation="MHADPQLDAEGRLTHLLSIDGLPKATVEQILDTAESFLEVGARD
                     VKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRLSADVINLDVSRSSTAKGETLLDTV
                     DNLVAMHADTFVVRHATSGAPYLIARHLRERGHDGVHVINAGDGRHAHPTQGLLDMYT
                     IRHYKRDFSQLTVAVVGDILHSRVARSDIAALRTLGAREIRAIGPRTLLPPEAEAMGV
                     KVFHDFDAGIEGCDVVIMLRLQSERMSGALLPSSREYFRQWGLTTERLARAKSDAIVM
                     HPGPMNRGIEIDSRVADGAQAVILPQVSFGIAVRMAVMSLTTGN"
     gene            4687..5961
                     /locus_tag="VIS73_13445"
     CDS             4687..5961
                     /locus_tag="VIS73_13445"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY5764207.1"
                     /translation="MPNHTPLPTAINNGRVIDPANGIDRITSVFLADGRIAAVGDQAP
                     DGFAAAQNIDATGCIVCPGLIDLAVHLPNVERELGAAAAGGVTRVVCPPDTSPPLDEA
                     GLVERLVRRAGEAGGTRVHPLGALTSGLAGTTLAEMATLAAAGCIAFSQGKRPIDDTL
                     VMMRALQYAASLGFAVWLQPEDYHLAQGGAAHDGAVAAQLGLPGIPICAETIAIATAL
                     ELVRESGARVHFRRLSSAAGIRLLAAGRAEGLPVTGDVSIYHLHLTDEAIGVFDSNAR
                     LSPPLRTARDRDALRAAIADGALQAIVSDHTPVSDDGKQLPFAEAEVGASGVELLLPL
                     AVALAETGGVPLARCLAAITCEPAALADLPGGTLSVGAAADVCVFDPADCWEVTPAAL
                     HSSGSNTPLAGQKLTGRVRLTIIDGRLVFSRG"
     gene            6136..7065
                     /locus_tag="VIS73_13450"
     CDS             6136..7065
                     /locus_tag="VIS73_13450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HEY5764208.1"
                     /translation="MFEIRRAVHSDIPAARQVLSLCPPPHRIVDNDAESTPPRSALYV
                     ATADGNLIGCAGFDLVADGHGLIRSLAVLPGFRKQHIGRHLVLRLLAHADEHAIGHLF
                     LLTDRAREYFMQLGFVGCSADCVPEAVRTAPRFRALFQPEAVLMQRAGERELCAENNA
                     STQAAVEAKRQFDSGFYCSESVLLAVARELGIDSPLLPAIATGFSSGVSRTWGTCGAV
                     NGAVLGLNLAFGRHQPSDSVEENYEAVRTMIGEFTRVCGSTQCSELLACDLDTAEGQR
                     VFKSHNLRTQCREYVGIAARLAAHIIQDRRRED"
     gene            complement(7072..7371)
                     /locus_tag="VIS73_13455"
     CDS             complement(7072..7371)
                     /locus_tag="VIS73_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEY5764209.1"
                     /translation="MQPDWLKAYDGGVLLTVHVQPGARQTATAGVHGDALKIRLQAPP
                     VDGKANAALLLYLADALGVAPRHLTLVSGQTSRRKRVRVEILAVEDVLAALAGAR"
     gene            complement(7378..7911)
                     /locus_tag="VIS73_13460"
     CDS             complement(7378..7911)
                     /locus_tag="VIS73_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY5764210.1"
                     /translation="MLGRIVEFLVSTLADLLAFVLLLRLFMQATRTSFANPLGEIVIA
                     LSNWIVRPLRRALPPLGPIDSASLLPAWLAQWLALVLILVTRGADLPMVPLLWVALVE
                     TLRIAVWVWVIALLAMAIISWVNPYSPFASPIAQLTRPILRPIQRVMPTVGRLDLSPL
                     VAILLLQVLLIVLDGLR"
     gene            complement(7961..8794)
                     /gene="proC"
                     /locus_tag="VIS73_13465"
     CDS             complement(7961..8794)
                     /gene="proC"
                     /locus_tag="VIS73_13465"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY5764211.1"
                     /translation="MVSGTAFLGGGNMATALVGGLLATGAEPGDILVADLNAAARQSL
                     ADRYGVRTTEKLVADDLREIDILVLAVKPQQMQEALAPLAGALSDQVVVSVAAGLPMV
                     VIGEWLGGYAKIVRAMPNTPALIRAGITGLCARPEVDAIGRRAAERLMHSVGTTVWFD
                     DEAMMDGVTAISGSGPAYFFLFIEALQQAGLAMGFSEEQARLLAIGTAEGAARLAVQS
                     DETVSELRARVTSKGGTTEAALHTMQERGVPDGIIAGAQAALARGRELGAQLARTGKQ
                     N"
     gene            complement(8799..9491)
                     /locus_tag="VIS73_13470"
     CDS             complement(8799..9491)
                     /locus_tag="VIS73_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEY5764212.1"
                     /translation="MVSIADNLQAVKGRIAAAARSCGRDPESVTLLAVSKTWPFESIA
                     AAFRCGQRAFGENYVQELVAKGEALSASPIEWHFIGPLQSNKTRPVAERADWVHSIDR
                     LRVAERLSQQRPAQRPPLQVCVQVNVSGEASKGGVAPGEAAALSIAVNALPRLRLRGL
                     MCIPEASDDPAVQRQAFAQLRQIADELRAAGLALDTLSMGMSHDLEAAIAEGATIVRV
                     GTAIFGERQRAG"
     gene            9595..10638
                     /locus_tag="VIS73_13475"
     CDS             9595..10638
                     /locus_tag="VIS73_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEY5764213.1"
                     /translation="MDITELLAFGVKNKASDLHLSSGLPPMIRVHGDVRRINLPPMEH
                     KDVHAMVYDIMNDGQRKIYEESLECDFSFEVPNLARFRVNAFNQHRGAGAVFRTIPSK
                     VLTLEELNCPKIFKDISEYPRGIVLVTGPTGSGKSTTLAAMVNHVNENEYGHILTVED
                     PIEFVHESKKCLINQREVGPHTLSFSNALRSALREDPDVILVGEMRDLETIRLALTAA
                     ETGHLVFGTLHTSSAAKTVDRIIDVFPAAEKEMVRAMLSESLRAVISQTLLKTKDGQG
                     RCAAHEIMIGTPAIRNLIREAKVAQMYSSIQTGQQYGMQTLDQNLAEMVKRNIVSPAE
                     ARSKAANKDNFAG"
     gene            10778..11914
                     /locus_tag="VIS73_13480"
     CDS             10778..11914
                     /locus_tag="VIS73_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY5764214.1"
                     /translation="MERDQALKFMHDLLRLMVSKKGSDLFITANFPPAIKIDGKVTPV
                     SNQQLTPQHTAELARSIMNDRQASEFEASHECNFAISPSGIGRFRVNAFFQQSRVGVV
                     CRTINTSIPKLEELGLPKILRDVAMTKRGLVMFVGGTGSGKSTSLAAMIGFRNENSYG
                     HIITIEDPVEYVHEHKNCIITQREIGVDTEDWTIALKNTLRQAPDVILIGEIRDRETM
                     EHAISFSETGHLCMGTLHANSTNQALDRIINFFPEDKRQQLLMDLSLNLRAMVSQRLL
                     PIRDGKGRTAAVEIMLNSPLISDLIFKGEVTEIKEIMKKSRELGMQTFDQSLFDLYEA
                     GKITYEDALRNADSVNDLRLQIKLHGKEASGKDLTAGLGHLDIV"
BASE COUNT         2188 a         4320 c         4023 g         1961 t
ORIGIN      
        1 caccagcgcc gcgccggcga gcagcgggtc gcgcccaaga tgcggcatgg cgccgggggc
       61 gccgcggcca tcgatgacga tctcgaattc gtcggcggcg gccatcaccg ggccggtgag
      121 gatcgcgaag tggttttcat cgaggccggg ccagttgtgc aggccgtaga agcgctcgat
      181 cggccagcgc tcgagcagcc cgtcggcgag catcgcggcg gcgccgccgc ggccttcctc
      241 ggccggttgg aagatcaggc agacggtgcc gcggaactgc gggtggcgcg cgagctgctc
      301 ggcggcgccc agcaacatcg ccgtgtggcc gtcgtggccg caggcgtgca tcttgccctc
      361 gtgcaccgag cgatgcgcaa aggtattggc ctcggccacc ggcaaggcgt ccatgtcggc
      421 acgcagcgcg atgaccggcc cttcgccatt cttcaacacg ccaacgaccc cggtctcgcc
      481 gatgccggtg accacctcgc agccgatgcg gcgcagttca cgggcaacga ggtcggcggt
      541 acgcaattcg gcgaaggcga gttccggatg cgcgtgaatg tcgcggcgca cgccgcgcac
      601 ggtatccacc agggtgggct cgggtgcagg aatgcgcgga acgaagctgt cagcggcggt
      661 gttcatcatc ggtcacctgt tgcagttgaa aggccattct acgtggcgcc ccgcagcacc
      721 gcgaaacgcg ccagcgcatc ctgtcggccc gtggcgtggt cgaccaccgg cggcggatag
      781 tccgccgggt gctgcgccgc cggcagcttc cacggcgcat ggatcgccgg cgccggcagt
      841 tcagccagtt ccggcaccca gcggcgcagg taggcaccgt cggcgtcgaa acgctgcgac
      901 tgggtcaccg gattgaagat gcggaaccag ggctgcgcgt cacaaccggt cgacgccgcc
      961 cactgccagt tgccggcgtt tgcggcgaga tcgtagtcga gcaggtgagc ggcgaaaaag
     1021 cgttcgccgt gctgccagtg aatgccgagg tgcttgcaga gaaacgaggc gacgaccatg
     1081 cgcacgcggt tatgcatgcg gccggtggcc agcagttcgc ggatgccggc gtcgaccagc
     1141 gggtagccgg tcgttccgct cttccatgcg gcaaaatcgg cggcggcctc gcctgccgga
     1201 tcgcgccaca cgacccgatc gtaggcgcgc ttgaagttct gtgtcgccga ctgtggaaag
     1261 gcggcgagga tcgcgaagaa gaaatcgcgc cagatcagct cgccaagcca gctgggcgaa
     1321 ttggcggcga gcgcggcgcg cacggcctgg cgcaccgaga gcgtgccgaa gcgcagatgc
     1381 ggggaaagca tcgaggtggc ggcctgcgcc gggaaatcgc gcgccgccgc gtagtcgggc
     1441 aagcgcggct ggaaggcggc cagcgctgcc agcgcgccgg cctcgctggc gacgacgccg
     1501 atatcggcca gcgccaccgg cgcgaagcca tacgcgtcga gcggcgcgag ctcctcgacc
     1561 gccggctgca gcacgcccgg tcgcagcgcc gggcgccgtt cgacaatcgc cgccggaatc
     1621 gcgtcgcggc gcttctccca cgcgcgacga tagggcgtgt agacggtgta tggccgaccg
     1681 gcggcggtga gcacttcgtc ggcctcgaag acgacctgat ccttgatcag caccaggtgt
     1741 cggccgtcgg cttcgagcgc gcggctgacg gcggcgtcgc gggccaacgc ctgcggctcg
     1801 acgtcgcggc cggcgtagac ggcgctgcag gccaaccggc gcgccaattg cgggatgacg
     1861 gtcggcgcat gaccgtggag cacggtcaag gttctggccc cggacgacaa gccaccccgg
     1921 gcggccagtt cggcgtcgag ggcagcgagc gcgtcgtgga tgaagttcag ccgccggtcg
     1981 tggcgatcgt cgagggcatc gaggatgtcg cggtcgaaga cgaagacgca atgcacgcgc
     2041 cgcgcctcgg cgaccgccgt ggccagcgtg gcgtggtcgg cgacgcgcag gtcgcggcga
     2101 aaccagacca gtgcgtcgtc gcgaaagggg ctgctcatgc gcgcctccgt cggcccgatg
     2161 acgggcgtgc gccgtgccgc agcgccgcat cgaccgtgta gaatttgcct gccatgccga
     2221 gcaccgaaag cctagccaac cagttcctca tcgccatgcc ggccatggcc gacccctttt
     2281 ttgccaaatc gttggtgttc atctgcgagc acaccgataa cggcgcgctc ggcatcatcg
     2341 tcaatcggcc tttggacatg acgctggcca agctgttcga aaaagttgat ctgccgctga
     2401 cgcagaacga cttccagcag cgcccggtct atttcggcgg cccggttcag accgaccgcg
     2461 gcttcgtgct gcatcggccg ctcggcggct tccagtcgac actaaggatc aacgacgcgc
     2521 tggggctgac cagctcgcgc gatgtactgc agaagatctc ggtcgaaggc gaccccaacg
     2581 acatcctggt gacgctgggc tacgccggct ggtcggccgg ccagcttgag aacgagatcg
     2641 cccagaacgc ctggctgacg gcgccggccg acgaggcgat cattttcgcc atgccgcccg
     2701 aggcgcgcat gccggcggcg atgcaaggcc tgggcatcag catgacgcat cttgccgaca
     2761 cggccgggca cgcctgagcc gatgcagccg gaacgccagc gcttgcccag cacgacgatc
     2821 ctggccttcg atttcggcga gcgccgtatc ggcgtcgcgg tcggcgagac cgagaccgcg
     2881 caggcgcatg cgctgaccac catcgaggcg gaagccaata tagaccgctt tgcggagatc
     2941 gccgcgctga tcgacgagtg gcggccggcg atgctggtgg tcggcttgcc gctgcacgtc
     3001 gacggcacgc cgcacgcgat gacggcgcgc tgcaagcgct tcgccaacca actgcacggg
     3061 cgcttcggcc tgcctgtggc aaccgccgac gaacgcctca cctcgcacga tgccgaggcg
     3121 ctgctgcacg aagtcggtcg cagcacgcgc gagcaacgcg cggtgatcga ctcgctggcg
     3181 gcccaactga tacttcaagg ctatctcaat gaccaacaag cccctgcctg acgccgagcg
     3241 gcaatgcgcc gaactcgccg cggcgattcg cccgcgcctg cgacccgatt cggcgctgat
     3301 cggcatctac agcggtggcg cctggatcgc cgaccggctc gccgaactgc tgtcggtcag
     3361 cgacgtcggg cagatagacg tctccttcta tcgcgacgac ttcgagaaga tcggcctgca
     3421 cccaaacgta cggcctacgg cgattccctt cgaggtcagc gagcggcacc tagtcctcgt
     3481 cgatgacgtg ctgcagtcgg gacgcacgac gcgcgccgcg ctcaacgtgc tgttcgactt
     3541 cgggcggcct gcctgtgtcg acctcgccgt gctcgccgac cgcggcggcc ggcatctgcc
     3601 ggtagcgccg acctggtgcg cctgggccgc ggagctgccg accagcgaaa tcctgatcct
     3661 cgatcgcggc gacgacggtc ggctcgcctg gcgcacggag gcccgagatg cacgctgatc
     3721 cgcaactcga cgccgagggc cgactgaccc acctgctgtc gattgacggg ctgccgaagg
     3781 caacggtcga gcagattctc gataccgccg aatcctttct cgaagtcggc gcccgcgacg
     3841 tgaagaaggt accgctcttg cgcggcaaga gcgtcttcaa cctgttcttc gagaactcga
     3901 cgcgcacgcg cacgaccttc gagatcgccg ccaagcggct gtcggccgac gtcatcaacc
     3961 tcgacgtctc gcgatcgtcc acggccaagg gcgagacgct gctcgacacc gtcgacaacc
     4021 ttgtggccat gcacgccgac acctttgtcg tccgccatgc gaccagcggc gcgccctacc
     4081 taatcgcccg ccacctgcgc gagcgcggcc acgacggcgt acacgtgatt aacgccggcg
     4141 acgggcgcca cgcgcacccg acccaaggcc tgctcgacat gtacacgatt cgccactaca
     4201 agcgcgattt ctcgcagctg acagtggcgg tggtcggcga catcctgcac tcgcgcgtcg
     4261 cgcgctccga catcgcggcg ctgcggacgc tgggcgcccg agagatccgc gcgatcggcc
     4321 cgcgcacgct gctgcccccc gaggccgagg cgatgggggt caaggtcttc cacgatttcg
     4381 acgccggcat cgaaggctgc gacgtggtca tcatgctgcg cctgcagagc gagcgcatga
     4441 gcggcgcgct gctgccctcg tcgcgcgagt acttccgcca gtggggcctg accacggagc
     4501 ggctggcgcg ggcgaaatcc gacgcgatcg tcatgcatcc gggaccgatg aaccgcggta
     4561 tcgagatcga ctcgcgcgtc gccgatggcg cgcaggcggt gatactgccg caggtcagct
     4621 tcggtatcgc cgtgcgcatg gcggtgatga gcctgaccac cggaaactga acggccccgc
     4681 gaaaacatgc ccaaccatac ccccctcccg actgcgatca acaacggccg cgtcatcgac
     4741 cccgcgaacg gcatcgaccg aatcacctcg gtttttcttg ccgacggccg catcgccgct
     4801 gttggcgacc aggcgccgga cggcttcgct gccgcgcaga acatcgacgc caccggctgc
     4861 atcgtctgcc cgggcctgat cgacctcgcc gtgcacttgc ccaacgtcga gcgtgaactc
     4921 ggcgccgccg cggccggcgg cgtcacccgc gtcgtctgcc cacccgatac ctcgccgccg
     4981 ctcgacgaag cgggcctggt cgagcgactg gtccgccgtg ccggcgaagc cggcggcacc
     5041 cgcgtccatc cgctcggggc gctgacgagc gggctcgccg gcaccacgct ggccgaaatg
     5101 gcgacgctgg ccgccgccgg ctgcatcgcc ttctcgcagg gcaagcggcc gatcgacgac
     5161 acgctggtga tgatgcgcgc gctgcagtac gcggcctcac tcggcttcgc ggtctggctg
     5221 cagcccgagg actaccatct agcgcagggt ggcgcggcgc acgacggcgc cgtcgccgca
     5281 cagctcggcc tgcccggcat ccccatctgc gccgaaacga tcgccatcgc cacggcgctc
     5341 gaactggttc gggaaagcgg cgcccgcgtc catttccgcc gcttgtcatc ggccgccggc
     5401 atccgcctgc tggccgcagg acgcgccgag gggctgccgg tcaccggcga tgtcagcatc
     5461 taccacctgc atctcaccga cgaggcgatc ggggtattcg attccaacgc gcgactgtcg
     5521 ccgccgctgc gtacggcgcg cgaccgtgat gcgctgcgcg ccgcgatcgc cgacggcgcg
     5581 ctgcaggcga tcgtctcaga ccacacgccg gtcagcgatg acggcaagca gctgcccttt
     5641 gccgaggccg aggtcggtgc cagcggcgtc gaactgctgc tgccgctggc ggtggcgctc
     5701 gccgaaaccg gcggcgtgcc gctcgcccgc tgcctggcgg cgatcacctg cgaacctgca
     5761 gccctcgccg atcttcccgg cggcacgctc tccgtcggtg ccgccgccga cgtctgcgtc
     5821 ttcgacccgg ccgactgctg ggaggtgacg ccggcagcac tgcacagcag cggcagcaat
     5881 acgccgctgg ccgggcagaa gctgaccggg cgcgtgcgcc tgaccatcat cgacggccgt
     5941 ctggtctttt cgcgcggctg atccgcgccc gccgccttgc ggcggcgcac cttgatccga
     6001 ccgcgccgag cgtctagtct tggctggatt gccgagtccg ctcgccggcc caccagaggc
     6061 taaacccgtg tctagtcggc cggggaaaca gaccggagcc ggccttcccc gacagccgac
     6121 cccggaggct gtccgatgtt cgagatccgc cgcgctgtcc acagcgacat ccctgccgcc
     6181 cggcaggtgt tgtccttgtg cccgccaccc caccggatcg tcgacaacga cgccgaatcg
     6241 acaccgccgc ggtcggcgct ctacgtcgcc accgccgacg gtaacctgat cggctgcgcc
     6301 ggcttcgacc tagtcgccga cggccacggg ctgatccgtt cgctggcggt cctgcccggc
     6361 ttccgcaagc agcacatcgg ccgccatctg gtgcttcgcc tgctcgccca cgccgacgag
     6421 cacgcgatcg gccacctgtt cctactcacc gaccgggcgc gcgagtactt catgcagctt
     6481 ggcttcgtcg gctgcagcgc cgattgcgtg cccgaagcag tgcgcaccgc gccgcgcttc
     6541 cgcgcactgt tccagcccga ggcggtattg atgcagcgtg ccggcgagcg cgagctgtgc
     6601 gccgagaaca atgcctcgac gcaggccgcg gtcgaggcca agcgacaatt cgattcgggt
     6661 ttctactgct cggagagcgt attgctcgcg gtcgcccgcg aactcggcat cgactcgccc
     6721 ttgttgccgg cgatcgccac tgggttttcg agcggcgttt cgcgcacctg gggcacctgc
     6781 ggcgccgtca atggtgccgt actcgggctc aacctagcct tcggccgaca ccagccgagc
     6841 gactctgtag aagagaacta cgaggcggtg cggacgatga tcggcgagtt cacccgggtc
     6901 tgcggatcga cccagtgcag cgaactgctc gcctgcgacc tcgataccgc cgaaggccag
     6961 cgcgtgttca agagccacaa cctgcgcacg cagtgccgcg aatacgtcgg catcgccgcc
     7021 cggctggcgg cccatatcat ccaggacagg cggcgcgagg actgaaccgc ttcagcgcgc
     7081 tccagccaac gcagcaagca catcctccac ggcaaggatt tcgacgcgca cgcgctttcg
     7141 tcgcgatgtc tgtccggaaa ccagcgtcag atggcgcggt gcgacgccca gcgcatcggc
     7201 cagatacagc agcagcgcag cattggcctt gccgtcgacc ggcggcgcct gcaggcggat
     7261 tttcagcgcg tcgccgtgga cgccggcggt ggccgtctgc cgggcgcccg gttggacgtg
     7321 cacggtgagc agcacacccc cgtcgtaggc tttcagccag tcgggctgca gcaaggctca
     7381 gcgcaggccg tcgaggacga tcaacagcac ctgcaacagc aggatcgcca ccagcggcga
     7441 caggtcgagc cgccccacgg ttggcatgac acgctggatc ggccgcagga tcggtcgcgt
     7501 cagctgggcg atcggcgagg cgaagggcga atagggattg acccaggaga tgatcgccat
     7561 cgccagcagg gcgatgaccc agacccacac cgcgatgcgc agggtctcga caagcgccac
     7621 ccagagcagc ggcaccatcg gcagatcggc gccacgcgtg acgaggatca gcaccagtgc
     7681 cagccactgc gcgagccagg ccggcagcag gcttgccgag tcgatcgggc cgagcggcgg
     7741 cagcgcgcgg cgaagcgggc gaacgatcca gttgctgagc gcgatgacga tttcaccgag
     7801 cgggttggca aagctggtgc gcgtcgcctg catgaacagg cgcaggagca gcacgaaggc
     7861 cagcaaatcg gcgagcgtgc tgacgagaaa ttcgacgatt cgacccagca tgcgggttct
     7921 cccctaagtg gttctgttgg tttcggcgcc gttgcggcgc ttagttctgt ttgccggttc
     7981 gtgccagttg cgcgccgagt tcacggccgc gcgccagtgc tgcctgcgcg ccggcgatga
     8041 tgccatcggg tacgccgcgc tcctgcatgg tgtgcaaggc cgcctcggtg gtgccgcctt
     8101 tcgaggtgac ccgtgcgcgc aactcgctga ctgtctcgtc gctctgcacc gcgagccgcg
     8161 ccgcgccttc ggcggtaccg atcgccagca ggcgcgcctg ttcttcgcta aaacccatcg
     8221 ccagcccggc ttgctgcagc gcctcgatga agagaaagaa atacgccggg ccgctaccgg
     8281 agatggcggt aacgccgtcc atcatcgctt catcgtcgaa ccagacggtg gtgccgaccg
     8341 agtgcatcag tcgctcggcc gcccgccgcc cgatcgcatc gacctcgggc cgcgcgcaga
     8401 ggccggtgat gccggcgcga atcagtgccg gcgtgttcgg catcgcgcgg acaatcttcg
     8461 cgtagccgcc aagccattcg ccgataacga ccatcggcag gccggcggca accgagacga
     8521 cgacctggtc gctcagtgcg ccggccagcg gcgccagcgc ctcttgcatc tgctgcggct
     8581 tgaccgccaa cactagaata tcgatctcgc gcagatcgtc ggcaaccagt ttttcggtcg
     8641 tgcgcacgcc atagcgatcg gcgaggctct gccgcgccgc cgcgttgaga tcggcaacaa
     8701 ggatgtcgcc cggctcggcg ccggtcgcca gcaggccgcc caccagcgct gtcgccatgt
     8761 tgccgccccc cagaaacgct gttccgctca ccatcgcttc accctgcccg ttgtcgttcg
     8821 ccaaaaatcg cggtgccgac gcgcacgatc gtcgcgccct cggcaatcgc tgcttcgaga
     8881 tcatgcgaca ttcccatcga cagcgtatcg agtgcaagcc ccgccgcgcg caattcgtcg
     8941 gcgatctgcc gcaactgcgc gaaagcctgg cgttgcacgg cggggtcgtc actcgcttcg
     9001 ggaatgcaca tcagtccgcg cagccgcagt cgcggcaaag cgttgaccgc gatggagagc
     9061 gccgcggcct cgcccggcgc cacgcctccc ttgctcgcct cgccactgac gttgacctgc
     9121 acgcagacct gcagcggcgg gcgctgcgcc ggtcgttgct gcgacagcct ttcggcgacg
     9181 cgcaggcgat cgatcgaatg cacccagtcg gcacgttcgg cgaccggacg cgtcttgttc
     9241 gattgcagcg gaccgatgaa atgccactcg atcggcgacg ccgaaagcgc ttcgcccttc
     9301 gccaccagct cctgcacgta gttctcgccg aaggcgcgtt gcccgcaccg aaatgccgcc
     9361 gcgatgcttt cgaagggcca agtcttgctc accgcgagca gcgtcaccga ctcggggtcg
     9421 cgcccgcaag accgcgccgc tgcggcgatc cggcctttta cggcttgcaa gttgtcagct
     9481 attgagacca taatcggcgc gcatttgggc gcctatgtcg cgcacgtttt tagccaatcc
     9541 agtatagcag cgacctcgct cgggatcgcc cgcgcagccc cacggagaca gcgaatggac
     9601 atcaccgaac tgctcgcctt cggcgtcaag aacaaggctt ccgacctgca cctttcatcg
     9661 ggcctgccgc cgatgattcg cgtgcacggc gacgttcgcc ggatcaacct gccgccgatg
     9721 gagcacaagg acgtacacgc gatggtgtac gacatcatga acgacgggca acgcaagatc
     9781 tacgaagagt cgctcgaatg cgacttctcg ttcgaagtgc ccaacctcgc gcgcttccgc
     9841 gtcaacgcct tcaaccagca tcgcggcgcc ggcgccgtgt tccggacgat tccgtcgaag
     9901 gtattgacgc tcgaagaact caactgcccg aagatcttca aggacatctc cgagtacccg
     9961 cgcggcatcg tgctcgtcac cggcccgacc gggtcgggca aatcaacgac gttggcggcg
    10021 atggtcaacc acgtcaacga gaacgagtac ggccacatcc tcaccgtcga agacccgatc
    10081 gaattcgtcc acgaatcgaa gaagtgcctg atcaaccagc gcgaggtcgg gccgcacacg
    10141 ctgtccttct caaacgcgct gcgttcggcg ctgcgcgaag accccgacgt gatcctcgtc
    10201 ggcgagatgc gcgacctgga aacgattcgg ctggcgctga ccgccgccga gaccggccac
    10261 ctggtgttcg gcacgctgca cacctcgtcg gccgcgaaga ccgtcgaccg gatcatcgac
    10321 gtctttccgg cagctgaaaa ggagatggtc cgcgcgatgt tgtcggaatc gctgcgcgcg
    10381 gtcatctcgc agacactgct caagaccaag gacggacagg gccgctgcgc ggcgcacgag
    10441 atcatgatcg gcaccccggc gatccgcaac ctgatccgtg aggcgaaggt cgcgcagatg
    10501 tactcgtcga tccagaccgg ccagcagtac ggtatgcaga cgctcgacca gaaccttgcc
    10561 gagatggtca agcgcaacat cgtgtcgcca gccgaagccc gcagcaaggc cgcgaacaag
    10621 gacaactttg caggatgacc atgtcagcct gcaaagttgc ggcgaacgct aacctgccgc
    10681 aggcagggta aggcgcgcag cgccggcggt agccgcaact tcgccagcac gcacgcacgc
    10741 acacaatcgg cgaccgacaa gtcgcaggag acaggcaatg gaacgcgacc aggcactcaa
    10801 attcatgcac gacctcttgc ggctgatggt cagcaagaag ggctccgacc tgttcatcac
    10861 ggccaacttc ccgccggcga tcaagatcga cggcaaggta acgccggtgt cgaaccagca
    10921 gttgacgcca cagcacaccg ccgagctggc gcgttcgatc atgaacgacc gtcaggcctc
    10981 cgagttcgaa gcgtcgcacg agtgcaactt cgcgatctcg ccctcgggca tcggccgctt
    11041 ccgcgtcaat gccttcttcc agcagagccg cgtcggcgtc gtctgccgga cgatcaatac
    11101 ctcgattccc aagctagaag aactcgggct accgaagatc ctgcgcgacg tggcgatgac
    11161 caagcgcggc ctggtgatgt tcgtcggcgg caccggctcg ggtaagtcga cctcgctcgc
    11221 ggcgatgatc ggtttccgca acgagaattc ctacggccac atcatcacca tcgaagaccc
    11281 ggtcgaatac gtccacgagc acaagaactg catcatcacg cagcgcgaaa tcggcgtcga
    11341 caccgaggac tggacgatcg cgctgaagaa cacactgcgg caggcgcccg acgtcatcct
    11401 catcggcgag atccgcgacc gcgagacgat ggagcacgcg atctcgttct cggaaaccgg
    11461 tcacctgtgc atgggaacgc tgcacgccaa ctcgaccaac caggcgctcg accgcatcat
    11521 caacttcttc cccgaggaca agcgccagca gctgctgatg gacctctcgc tgaacctgcg
    11581 ggcgatggtc tcgcagcggc tgctgccgat ccgcgacggc aagggccgca ccgccgcggt
    11641 cgagatcatg ctcaactcgc cactgatctc cgacctgatc ttcaagggcg aagtcaccga
    11701 gatcaaggag atcatgaaaa agagccgcga actgggcatg caaaccttcg accagtcgct
    11761 gttcgatctc tacgaagccg gcaagatcac ctacgaagat gcgctaagga acgccgactc
    11821 ggtcaacgat ttgcgcctgc agatcaagct gcacggcaag gaggcgtctg gcaaggatct
    11881 aaccgccggc cttgggcacc tcgacatcgt ctgagcagag gctcgccgat actgcgttgg
    11941 ctgcgctcgg gcgctcctcg ccgtcgcact accactgtgt ctcgtcactc cctcgcttgc
    12001 cgcctcgtct cggcgtcgcc tggcggccat cgggtcagcg cgtcctgtac acgagttggt
    12061 tctggttggc gcaccgctgt atcttcacgt catgtaggcg gcgcgggaac tggaaggccg
    12121 aggtcggcca tcagccgacc ttcgatccag tttcccgtgg ggccggttaa gacttaatcc
    12181 tgtcattagc aatgacatag aaatgggctt tgaagggctg gtattcgggt caatccgtcg
    12241 tttaaaacgt agaacgtaat tcacaagaca agctggacca gagcatcacc caacagttga
    12301 gccagaaatc gctccacgat agcgccccag atttttggat atagcacgac cctcaaggtt
    12361 gcgcttcggt ttgctacgtg taatgaccat gggtttaaag cggttgatac aggcgcttct
    12421 ttgacatacg ctgaatgcga tgcttaggat ttctggtcct caatctcgct gggccgctcc
    12481 caagttcacc ag
//
LOCUS       DATDAA010000449         3981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_242754,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000449 DATDAA010000000
VERSION     DATDAA010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242754"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VIS73_13485"
     CDS             <1..247
                     /locus_tag="VIS73_13485"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HEY5764215.1"
                     /translation="SKKYESGTGWPSFWEALPGALGTKTDYILLYPRTEYHCARCGGH
                     QGHVFNDGPQPSGKRYCNNGVALRFVPRGEPLPALLT"
     gene            244..879
                     /gene="msrA"
                     /locus_tag="VIS73_13490"
     CDS             244..879
                     /gene="msrA"
                     /locus_tag="VIS73_13490"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY5764216.1"
                     /translation="MRARHWLVAGALALAGLATIAADKSEMPATGKAAEAPAGSAAAI
                     LAGGCFWCTESDFEKLPGVISAESGYINGFVANPTYKQVSAGVTGHTEAVRIVYDPAR
                     VSYAQLLDHFWKNHDPTVKDQQFCDRGSQYRAGIYPLDTEQQAAAEASLRAVEKSGRF
                     ARIYTEIVPAKTFYPAEEYHQDYYLKNPIRYNYYRTGCGRDARLAELWGTK"
     gene            complement(884..1603)
                     /gene="fabG"
                     /locus_tag="VIS73_13495"
     CDS             complement(884..1603)
                     /gene="fabG"
                     /locus_tag="VIS73_13495"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY5764217.1"
                     /translation="MKRALITGGSGGIGAAICRRLAADGHHVIVHGNRNLDTAEALAQ
                     EIIAAGGSAEAVSFDVTDAAASAVAIEGLLEPGAIQVLVNNAGIHDDAVFPGMRAEQW
                     HRVIDVSLNGFFNVTQPLTLPMIRSRWGRIINISSVAAIAGNRGQVNYAAAKGALHSA
                     TKSLALEVASRGITVNAVAPGIIDTGMIAGTFDAATIDKLVPVKRAGRPEEVANLVGF
                     LASAQADYITGQIISINGGLI"
     gene            complement(1600..2073)
                     /locus_tag="VIS73_13500"
     CDS             complement(1600..2073)
                     /locus_tag="VIS73_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxylacyl-ACP dehydratase"
                     /protein_id="HEY5764218.1"
                     /translation="MLSDKAMSTLDRDAIAARIPHTGTMCLLDSADRWNDESIRCTTE
                     SHRRSPHPLAAAGRLGIANTIEYAAQAMALHGALSAAAIDRPRAGFLASVRGVEFFAD
                     RLDDIAAPLTVEAERLSGDGNSVIYGFRVAAGERTLAQGRAAVVLDAGARLEQAS"
     gene            complement(2067..2867)
                     /locus_tag="VIS73_13505"
     CDS             complement(2067..2867)
                     /locus_tag="VIS73_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEY5764219.1"
                     /translation="MSATRLEAWISGVGVVGPGLVDWPSTRAVLAGEQAYLHQPTALV
                     APALLPAAERRRTGRAVRLALAVGLEAIADAELDPAQLATVFTSSGGDGENCHLICEA
                     LASDDRQISPTRFHNSVHNAPAGYWSIATGAMAPSSCLCSFDASFAAGLFEAMVQATA
                     DEAPVALLAYDTGYPEPLLAVRPIAEAFGVSMVISPRAPARGWKLTAWLSDAPAQAMP
                     DTTLEGLRQTIPAARALPLLQALAGGKPTTVTLSAWDDVQLTIEVAAC"
     gene            complement(2864..>3981)
                     /locus_tag="VIS73_13510"
     CDS             complement(2864..>3981)
                     /locus_tag="VIS73_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY5764220.1"
                     /translation="RAARSGLARCAFETVDLPTWIGEVAGVDAQRLPAEFASFDCRNN
                     RLAWLGLQQDGFVDAVRAAIARHGADRVGVFLGTSTAGILETEIAYRHRDPATGALPA
                     DFDYRRSHNTYSVGDFVRTAFGLRGPAAVVSTACSSSAKVFASASRAIAAGLIDAAIV
                     GGVDSLCLTTLYGFNSLELCSPTPCRPFDATRDGISIGEAAAFILVEPAKPDDDAICL
                     LGYGESSDAHHMSSPHPDGLGARLAIEAALRRAALAPADIDYVNLHGTATQSNDAAED
                     KAVYAVFADRVVASSTKGATGHTLGAAGGVEAIYAALAIENSFAPAGVNTTTIDPVLQ
                     TRYLLENRQMPVRHVVSNSFGFGGTNASLVIGRRRPA"
BASE COUNT          635 a         1263 c         1484 g          599 t
ORIGIN      
        1 ctcgaagaag tacgagagcg gtaccggctg gccgagcttc tgggaggcgc tgccgggtgc
       61 gctgggcacc aagaccgact acatcctgct ctatccgcgc accgaatatc actgcgcgcg
      121 ttgcggcggg catcaggggc acgtcttcaa cgatggcccg cagccctcgg gcaagcgcta
      181 ttgcaataac ggcgtcgcgt tgcgcttcgt gccgcgtggc gagccgctgc cggcgctgct
      241 gacatgaggg cgcggcactg gctggtagcc ggggcgctgg cgctcgccgg gctggcgacg
      301 atcgccgccg acaagagcga gatgccggca accggcaagg ctgccgaggc gccggcgggc
      361 agcgcggcgg cgattctcgc cggcggctgc ttctggtgca ccgagtcgga tttcgaaaag
      421 ctgcccgggg tgatcagcgc cgaatcgggc tacatcaacg gcttcgtcgc caacccgacg
      481 tacaagcagg tgagcgccgg cgtcaccggc cataccgagg cggtgcgcat cgtctacgac
      541 ccggcgcggg tgagctacgc gcaactgctg gatcatttct ggaaaaacca cgatccgacg
      601 gtcaaggacc agcagttttg cgaccgcggc agccagtacc gtgccgggat ctacccgctc
      661 gacaccgaac agcaggcggc cgccgaggcg agcctgcgag cggtggagaa aagcgggcga
      721 ttcgcgcgca tttacaccga gatcgtgccg gcgaagacct tctacccggc cgaggagtac
      781 caccaggact actacctgaa gaatccgatc cgctacaact actaccgcac gggctgtggc
      841 cgcgacgcgc ggctggcgga actgtggggg acgaagtagc gcctcagatc agcccgccgt
      901 tgatcgagat gatctggccg gtgatgtagt cggcctgcgc cgaggcgagg aagccgacga
      961 ggttggcgac ttcctcgggg cggccggcgc gcttgaccgg gactagcttg tcgatggtcg
     1021 ccgcatcaaa ggtgccggcg atcatgccgg tgtcgatgat gcccggcgcg accgcgttga
     1081 cggtgatgcc gcggctggcg acttcgagcg ccagcgactt ggtcgccgaa tgcagcgcgc
     1141 ccttggcagc cgcgtagttg acctggccgc ggttgccggc gattgccgcg accgaagaga
     1201 tgttgatgat gcggccccag cggctgcgga tcatcggcag cgtcagcggc tgggtgacgt
     1261 tgaagaagcc gttgagcgag acgtcgatga cccggtgcca ctgctcggcg cgcatgcccg
     1321 ggaacaccgc atcgtcgtgg atgccggcgt tgttgaccag gacctggatc gcaccgggct
     1381 caagcagccc ctcgattgcc acggcgctgg ctgcggcatc ggtgacgtcg aaactgacgg
     1441 cctcggcgct gccgccggcg gcgatgatct cctgcgccag cgcctcggcg gtgtcgagat
     1501 tccggttgcc gtggacgatg acgtgatggc cgtcggcggc cagtcgtctg cagatcgcgg
     1561 cgccgatgcc gccgctgcca ccggtgatga gcgcgcgctt catgaggcct gttcgaggcg
     1621 agcgccggcg tcgagcacga cggcggcgcg tccctgcgcc agcgtgcgct cgccggctgc
     1681 gacgcggaat ccgtagatca cgctgttgcc gtcgccggat agccgttcgg cttcgacggt
     1741 gagcggcgcg gcgatgtcgt cgaggcgatc agcgaagaat tcgacaccgc gcacgctggc
     1801 cagaaaaccg gcacgcggcc tgtcgatcgc ggcggcgcta agcgcgccat gcagcgccat
     1861 cgcctgcgcc gcgtactcga tggtgttggc gatgcccaag cggcccgcgg cggcgagcgg
     1921 gtgtggcgaa cgacgatggc tttcggtggt gcagcggatc gattcgtcgt tccaccgatc
     1981 agccgaatcg agcaggcaca tggtgccggt gtgcgggatg cgcgcggcga tggcatcgcg
     2041 atccagcgtg gacattgcct tgtcgctcag cacgctgcga cctcgatggt gagttgtacg
     2101 tcgtcccagg ccgacagagt aaccgtcgtc ggcttgccgc cggcgagcgc ctgcagcaag
     2161 ggcagtgcac gggcggccgg gatcgtctgc cgcaggcctt cgagcgtcgt gtcgggcatc
     2221 gcctgggccg gcgcgtcgct gagccaggcg gtgagtttcc agccgcgcgc cggtgcccgc
     2281 ggactgatga ccatcgaaac cccgaaggct tcggcgatcg ggcgcacggc gagaagcggc
     2341 tccgggtagc cggtatcgta ggcgagcagc gcgaccggcg cttcgtcggc cgttgcctga
     2401 accatcgcct cgaacaggcc ggcggcgaaa cttgcatcga aggagcacag gcaggaggaa
     2461 ggcgccatcg cgccggtggc gatgctccag tagcccgccg gcgcgttgtg taccgaattg
     2521 tgaaagcggg tcggcgagat ctgccggtcg tccgaggcga gcgcctcgca gatcaggtga
     2581 cagttctcgc cgtctccgcc ggacgaagtg aagacagttg cgagctgcgc cggatcgagc
     2641 tcggcatcgg caatcgcttc gaggccgacg gccagcgcca gcctgaccgc gcggccggtg
     2701 cggcgccgct cggccgcggg cagcagggcc ggcgcgacga gtgcggtcgg ctggtgcagg
     2761 taggcctgtt cgccggcgag cacggcgcgc gtgctgggcc agtcgacgag gccgggaccg
     2821 acgacgccga cgccgctgat ccaggcttcg agccgggtgg cgctcatgcg ggcctccgtc
     2881 ggccgatgac gaggctggcg ttggtgccgc cgaagccgaa ggaattggag acgacgtggc
     2941 gaaccggcat ctggcgattc tcgagcaggt agcgcgtctg cagcaccggg tcgatcgtcg
     3001 tcgtattgac gccggcgggg gcgaagctat tctcgattgc cagcgccgcg tagatcgcct
     3061 cgacaccacc ggcagcgcca agcgtgtggc cggtcgcgcc cttggtcgaa ctggcgacga
     3121 cgcggtcggc gaagaccgcg tagacggcct tgtcctcggc ggcatcgttt gattgcgtcg
     3181 ccgtgccgtg caggttgacg tagtcgatgt cggccggtgc gagcgcggcg cggcgcaatg
     3241 ccgcctcgat ggccagtcgc gcaccgagcc catcgggatg tggcgaggac atatggtgcg
     3301 cgtcgctcga ctcgccgtag ccgagcagac agatcgcgtc gtcgtcgggc ttggcaggct
     3361 cgacgaggat gaaggcggcg gcctcgccga tcgagatgcc gtcgcgcgtc gcgtcgaagg
     3421 ggcggcaggg ggtcggcgag cagagttcga gcgagttgaa gccgtagagc gtggtcaggc
     3481 agaggctgtc gacgccgccg acgattgccg cgtcgatgag cccggcggcg atcgcccgcg
     3541 aggccgaggc gaagaccttg gcggaggacg agcaggccgt cgagacgacg gcggccgggc
     3601 cgcgcaggcc gaaggccgtg cgcacgaagt cgccgaccga gtaggtgttg tggcttcggc
     3661 ggtagtcgaa gtctgccggc agcgcgccgg ttgcaggatc gcgatggcgg taggcgatct
     3721 cggtctcgag gatgccggcc gtgctggtgc cgaggaagac gccgacgcga tcggcaccgt
     3781 ggcgggcaat tgccgcgcgc acggcatcga cgaaaccatc ctgctgcagg ccgagccagg
     3841 caagccggtt gttgcggcaa tcgaaggacg cgaattcggc cggcaggcgc tgcgcgtcaa
     3901 ccccggcgac ctcgccaatc caggtcggca ggtcgaccgt ttcgaaggca cagcgagcca
     3961 gtccgctgcg cgcggcgcgt a
//
LOCUS       DATDAA010000450        15436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_102810,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000450 DATDAA010000000
VERSION     DATDAA010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 15436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15436
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102810"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VIS73_13515"
     CDS             complement(<1..551)
                     /locus_tag="VIS73_13515"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5764221.1"
                     /translation="MSVATPGLFDAHLHIIDPAFPLVANQGYLPPPFTVADYRAQAEA
                     LGITGGAVVSGSFQSFDQGYLLAALAALGEGFVGVTQLPADASDREIDRLDDAGVRAL
                     RFNLRRGGPLDPAQIERCARHVFARHGWHVEFYVDAASLAEMVPLLRRLPAVSIDHLG
                     LEAAALPTLLPLIESGVRVKATG"
     gene            complement(548..1027)
                     /locus_tag="VIS73_13520"
     CDS             complement(548..1027)
                     /locus_tag="VIS73_13520"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEY5764222.1"
                     /translation="MTDMSTVRCQLLLAPLLALTLLAGCASTPPPLPDPVALDQAPAA
                     ASPLDGIDWRLVQLSGQWVILADTSRTPTLRFDGASQKASGFDGCNRFNGRLRVLGSH
                     LRVGPLASTRMACPASMAPERAFVVAAESAVHWQRQQDLLELLDAAGQPLLLLRAGP"
     gene            complement(1130..2131)
                     /locus_tag="VIS73_13525"
     CDS             complement(1130..2131)
                     /locus_tag="VIS73_13525"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEY5764223.1"
                     /translation="MPLPDATAVGPQPFGALDPQTLLAALDAVGFATDGRLQALNSFE
                     NRVYQVGLDDSPPVIAKFYRPGRWSDAQIIEEHAFLAELVDDEVPVVAPLARDGVTLH
                     HADGLRFAVFPRQGGRAPELDRPGAIEQMGRFLARTHVVGGRAPFAERPALSVDDYGR
                     QPLAELAASERIPPESAPVYLALAAQALDAAEIAWQRAGTPRRLRLHGDCHLGNVLWT
                     DGGPHFVDFDDSRQGPAIQDLWMLLSGDHADQERQLAALLAGYETFREFDRAELHLVE
                     ALRTLRLIHHAAWIARRWDDPAFPPAFPWFESPRYWEARILELREQIAALDEAPLSA"
     gene            complement(2135..3055)
                     /locus_tag="VIS73_13530"
     CDS             complement(2135..3055)
                     /locus_tag="VIS73_13530"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEY5764224.1"
                     /translation="MTRTARYARTATSAALLLAGLLGFGTTAGAATYADIAPILQVRC
                     VMCHSGDNAPLGLKLDSLEALKSGSKNGPIAMASAPDDSELIRRLTGNKQPRMPMTGP
                     PFLSDAEVAQFRDFVAAGMPAGSAAAAPAAAPTRPKSEFATYDDVAPIFARRCAKCHT
                     DSGLMGPPPEGYRLTSHAATLATADRVRVVPGNPGASELLRRVRGDAYPRMPFDGPPW
                     LAADEVALIERWIVQGARDSAGKTAAAPVGARLRLHGRLESGDRLDELPLAINRGTRI
                     DKTPRPGDYVEVRATLGSDGAVLADRIRRR"
     gene            3207..3407
                     /gene="thiS"
                     /locus_tag="VIS73_13535"
     CDS             3207..3407
                     /gene="thiS"
                     /locus_tag="VIS73_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY5764225.1"
                     /translation="MQIIVNGQDRLVQPGATVADLVAALALAGRRIAVELNGEIVPKS
                     SHATRSLGEGDRVEIVVAVGGG"
     gene            3426..4223
                     /locus_tag="VIS73_13540"
     CDS             3426..4223
                     /locus_tag="VIS73_13540"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY5764226.1"
                     /translation="MDQQQDFLDIAGKRYGSRLLVGTGKYRDFAETRAAVDASGAEIV
                     TVAIRRTNIGQEPGQPSLLEVLPADQFTYLPNTAGCYTADEAIRTLRLARELLDGHDL
                     VKLEVLGDSQTLFPNMPETIRAADILVRDGFKVMVYCSDDPIQARMLEEIGCVAVMPL
                     ASLIGSGMGILNPWNLQLIIDQAKVPVIVDAGVGTASDAAIAMELGCDGVLMNTAIAK
                     ARQPVDMARAMRLAVEAGRLAYRAGRMPKKTYAAAPSSPTEGLIGKS"
     gene            4223..4954
                     /gene="trmB"
                     /locus_tag="VIS73_13545"
     CDS             4223..4954
                     /gene="trmB"
                     /locus_tag="VIS73_13545"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEY5764227.1"
                     /translation="MTARRDLATEAPFSVEDEKARPVRSFVLRQGRMSEAQTRAAATM
                     MPTIGVPFRAEPLNFAALFGRVAPVVVEIGFGMGHTTADIAAALPATDFLGVEVHLPG
                     VGSLCKRIADDGLDNIRIVRHDAVEVLATMVPPASLAGIHVFFPDPWPKKRHHKRRLI
                     QPGFVALAASRLAPGGYLHCATDWEEYAQQMLDVLSAEPLLDNEADGYAPRPAYRPET
                     RFEARGLRLGHGVWDLRFIRREVST"
     gene            4951..5757
                     /locus_tag="VIS73_13550"
     CDS             4951..5757
                     /locus_tag="VIS73_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY5764228.1"
                     /translation="MRRWLVAAVIALSVSATALATPRHAPVPGGVAWVPLPVTGDEAP
                     TAQFGDAPLLVVREGDSWLALVGLPIDQVPGEATIRWAQGAAAGELAISVAAKAYPTQ
                     ELRVAPKYVEPDPETLVRIGKERARVNEIKALHSLELPDLGFVAPADGPLSSRYGLRR
                     VFNGQPRSPHLGLDIAVPKGTPVRTPSVGTVVDVGDYYYWGRFVAVDHGRGLVTLYAH
                     LSQVGVTVGQRLAAGEVLGAVGATGRVTGAHLHWAVILNGHAVDPELFLP"
     gene            5978..6283
                     /locus_tag="VIS73_13555"
     CDS             5978..6283
                     /locus_tag="VIS73_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5764229.1"
                     /translation="MKAFTVALMAVAGLAISGQAAADEALAKAKNCLTCHQDAIKIVG
                     PSYKDVAAKYKGQKDAVATLAAKIKAGGKGNWGEVPMPPNNVTDEEAKKLAAWVLAK"
     gene            6507..7613
                     /locus_tag="VIS73_13560"
     CDS             6507..7613
                     /locus_tag="VIS73_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5764230.1"
                     /translation="MAAFAMLPTSASADTVVKIGHVAPLTGPIAHLGKDNENGARLAI
                     EHANAQGIMIGGQKVKFELVGEDDQADPKTGTVVAQKLVDAKVAGVVGHLNSGTTIPA
                     SAIYNQAGLPQISPSATNPKYTQQGYKTAFRVMANDVQQGSVIGTYAVKGLGAKTIAI
                     IDDRTAYGQGLADEVEKAAKAAGAKVVAREFTNDKATDFRAILTKIKSTKPDVIFHGG
                     MDATGGPMLKQMRDLAIKSTFTTGDGGCSPEIIKLAGKAAEGVICTQAGLPVDKLPNA
                     QRFMTDFTKKYGQIQIYSPYSYDAVMVLIDAMKRANSTDPAKYLPMVASTNLDGVTGK
                     IAFDDKGDVRNGAITVFKIEGGKLNTLKIEGGSM"
     gene            7730..8656
                     /locus_tag="VIS73_13565"
     CDS             7730..8656
                     /locus_tag="VIS73_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5764231.1"
                     /translation="MDIFLQQLINGITLGAVYALVALGYTMVYGIIGLINFAHGEVVM
                     IGTMVAFTVITALAASTGMPPLLIFSCGALAAMAACMLLGFGLERVAYRPLRHAPRLT
                     PLITAIGMSIVLQNVAMIVWGRNYINFPQMLDKQIYEVGGAVISNVQIGIIVLAMMTM
                     GGLLMLIYRTRLGAAMRATAQNPHVAGLMGIDINRVISLAFVIGSLLGAVAGLMVGTY
                     YEIAHYQMGFMLGLKAFTAAVLGGIGNVGGAMAGGLLLGVIEALGAGYIGDLTGGFLG
                     SHYQDIFAFVVLICVLMFRPSGLLGERQGDRA"
     gene            8653..9720
                     /locus_tag="VIS73_13570"
     CDS             8653..9720
                     /locus_tag="VIS73_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5764232.1"
                     /translation="MSPEFIERHRMLLFVTSGILLAILPFIVDAAAGQAWVRILNFAI
                     LYVFLALGLNIVVGLAGLLDLGFIAFYAVGAYLYALFASPHFDLHTSFFIMLPFCAAL
                     ACLFGVMLGAPTLKLRGDYLAIVTLGFGEIIRIFLNNLNAPINVTNGAQGITRIDPVS
                     IGEMQFNTTTHFLGIDFTSPTKYYYLLLALAFFVILVNIRLHHSRIGRAWQAIREDEV
                     AAKAIGINTRNVKLLAFAMGASFGGIAGAVFSASQGFVSPESFSLLESITILAMVVLG
                     GMGHIPGVILGAVLLTILPEALRYGIEPLQHALFGKSYLDPESLRMLMFGLALVLVMR
                     YRPAGLWPSPERRRELAERED"
     gene            9724..10500
                     /locus_tag="VIS73_13575"
     CDS             9724..10500
                     /locus_tag="VIS73_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5764233.1"
                     /translation="MADQELLLEARGVAKHFGGVKALQDVSLTIRRGEIYGLIGPNGA
                     DKTTFFNCLTGLYRNEHGGFVFDGEPLEADAPHLAAIRGIARTFQNIRLFGNMTALEN
                     VMVGRHLRTRANVFGAIFHTAATLAEERAISRRALELMHYVGIADRAGDFAKNLSYGD
                     QRRLEIARALATDPKLLALDEPAAGMNGAETEDLRVLIEGIRRDGVTVLLIEHDVKLV
                     MGLCDRVAVLDYGEKIAEDVPAVVQRDPRVIEAYLGGSPD"
     gene            10521..11228
                     /locus_tag="VIS73_13580"
     CDS             10521..11228
                     /locus_tag="VIS73_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5764234.1"
                     /translation="MLEVTGLQVAYGAIQAVRGITFHIEKGEMVALIGANGAGKTTTL
                     KALARMHDVAGGRISYCGEDITRIPPHQLVRKGIALVPEGRGMFPRLTVHENLMMGAF
                     SRNDSREIAADIDQAFERFPRLRERSQQLAGTLSGGEQQMLAIGRALMSRPKLLLLDE
                     PSMGLAPIMVQKIFEVVKEVAKAGMTILLVEQNARLALQVSRRAYVMESGEITISGES
                     VKLLDDPKVRAAYLGAE"
     gene            complement(11313..12482)
                     /locus_tag="VIS73_13585"
     CDS             complement(11313..12482)
                     /locus_tag="VIS73_13585"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY5764235.1"
                     /translation="MSDTYFSHQPILGKNGKLTANRLIVHAADSRSAVAALRQVEEFW
                     PMNQSVSFFLSLGNARVDPALFDWLLPENVILEIRGSDLAGEAGVATAQALRDNAAPL
                     CIDVDPDVGNANGANLSFRFFALDGAQVPIAQMQALTVKLRQLGTPITLNVATPTDFK
                     ACIDSGITAASGWFCKQPSSTMAKTLAPAQAQIVRVLNLVRNNADVPQIETALKQDVA
                     LSYKLLRYINSAGFGLSCEIQSFRHAVTILGYDKLNKWLSLLLVTASKDPLAPAQMHA
                     ALVRARLMELLGQGLVDKGELDNLFITGAFSLLDVLLGVSMDKALDAMNLPGPISEAL
                     LQRAGSYAPFLDLALAVEVGDAKAIATRAQALGLTAAKVNAALLQAIDFSATMAV"
     gene            complement(12482..13516)
                     /locus_tag="VIS73_13590"
     CDS             complement(12482..13516)
                     /locus_tag="VIS73_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HEY5764236.1"
                     /translation="MSYRVVVRDSDLAFTTMADESILEAGLRQGINLPYGCRNGACGA
                     CKTLVTEGRVDHDGADPATLTDDEKAQGFTLLCCARAESDIEIVCREVAASAIPAKTF
                     PARIEAMDRLASDVMRIALRIPATERLQFLAGQYLEILLKNGERRAFSMANPPHDDAL
                     IELHVRHVPGGEFTDQVFATMKVRDILRVHGPLGSFTLDEKSERPIVCVAGGTGFAPI
                     KAIIEHALHTGCTRPIRLYWGARDRAGLYAIALAERWAREHAQLHFVPVLSEPAAGDD
                     WVGRRGLVHEAVIDDLGDSGQLAASDVYICGAPAMISAAREDFTTRCGLPAERFFADA
                     FTFAHGTESS"
     gene            13548..14429
                     /locus_tag="VIS73_13595"
     CDS             13548..14429
                     /locus_tag="VIS73_13595"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5764237.1"
                     /translation="MIRVLIVGAGDVARRLHPLLARRFRVFVLLRNADKAGAWRAAGA
                     TPIAGDLDDRRSLDRAAALAQWIVHLAPPPSGGADDPRTAHLLAALRRRASLAQRLVY
                     ISTSGVYGDCGGARIDESKTPSPKTPRGRRRLAAERRVRSYAKSRRASILRVPGIYAA
                     DRLPLARLQSGTPALQANDDVFTNHIHADDLAASVRQALLYGRSGRVYHVVDDSEQRM
                     GDYFDAVADAFGLARPPRISRADAARQIDPGMLSFMSESRRLDNSRMKRELRLRLRYP
                     NTAAGIAAALDPERLPC"
     gene            14423..14839
                     /locus_tag="VIS73_13600"
     CDS             14423..14839
                     /locus_tag="VIS73_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY5764238.1"
                     /translation="MLTLKALHLIFVTSWFAGLFYLPRIFVNLALVPAGSVAERDRLL
                     LMAGKLYRFMLPLAVLALVFGIWLWLAYGYGGGWLHAKLALVVVLVIYHAVCGRHLKQ
                     FIAGNNTRSHVWYRWFNELPVLVLAVVVFLVVLKPF"
     gene            14848..>15436
                     /locus_tag="VIS73_13605"
     CDS             14848..>15436
                     /locus_tag="VIS73_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEY5764239.1"
                     /translation="MERFFASCPRGLEALLAEDLVGWGASEVRAEGGGVAFAGDWPVC
                     WRANLHSRLATRILCQVAEGAYQKEDDIYRLARAVDWAARFSVDRSIRVAITAVRSPL
                     KSIDFTTLRIKDAVCDRFRQDCGRRPDVDTRAPDVRIHAFLNAERATLYLDTSGEPLY
                     HRGLRQKTVDAPLKENLAAGILRLTGWQPGVALFDP"
BASE COUNT         2499 a         5040 c         5238 g         2659 t
ORIGIN      
        1 aagccggtgg ccttgacgcg cacacccgat tcgatcagcg gcagcagcgt cggcagcgcc
       61 gcggcttcga ggccaaggtg gtcgatgctc accgccggca gtcggcgcag cagcggcacc
      121 atctcggcga gcgaggcggc atcgacgtag aactcgacgt gccagccgtg tcgcgcgaag
      181 acgtgccggg cgcagcgctc gatctgcgcg gggtcgagcg ggccgccgcg ccgcaggttg
      241 aagcgcagcg cgcgaacccc ggcgtcgtcg agccggtcga tttcgcggtc gctggcatcg
      301 gcgggcagct gggtgacgcc aacaaagccc tcgccgagcg ccgccagcgc ggcgagcaga
      361 tagccttgat cgaaagactg gaaggagccc gagaccacgg cgccgccggt gatgccgagt
      421 gcctcggcct gcgcccggta gtcggcgacc gtaaacggcg gcggcaggta gccctggttg
      481 gcgaccagtg gaaaggctgg gtcgatgatg tgcaggtggg cgtcgaacag gcccggcgtg
      541 gccacgctca tggcccggcg cgcagcagta gcagcggctg gccagcggcg tcgagcagtt
      601 cgagcagatc ctgctggcgc tgccagtgga cggccgattc ggcggcgacg acgaaggcgc
      661 gctcgggcgc catgcttgcc gggcaggcca tccgggtcga tgccagcggg ccgaccctga
      721 ggtggctgcc gaggacgcgc aggcggccgt tgaagcggtt gcagccgtca aagccgctgg
      781 ccttctggct ggcgccgtcg aagcgcagcg tcggcgtgcg gctggtatcg gcgagaatca
      841 cccactgccc cgagagctgg accagccgcc agtcgatgcc gtcgagtggc gaggcggcgg
      901 ccggtgcctg gtcgagggcg accgggtcgg gcagcggcgg cggggtgctg gcacagccgg
      961 ccagcagggt cagcgccagc agcggcgcga gcaggagttg gcagcgaacg gtgctcatgt
     1021 cggtcatcag gtaaatagcg acgcgccgac tgtagcgcga aacgcctcgc ctgccgactg
     1081 cagccaaggc gccggcaggt gctggtgcct ggccattggc gacgcgccgt caggcggaca
     1141 gcggtgcctc gtcgagcgcg gcgatctgct cgcgtaactc gagaatgcgc gcctcccagt
     1201 agcgcggcga ctcgaaccac ggaaacgccg gcggaaacgc cgggtcgtcc cagcgacggg
     1261 cgatccacgc cgcgtggtgg atcagccgga gcgtgcgcag tgcctcgacc agatggagct
     1321 cggcgcgatc gaattcgcgg aaggtttcgt agccggcgag cagggcggcg agctgccgct
     1381 cctgatcggc gtgatcgcct gagagcagca tccagagatc ctggattgcc ggcccctgcc
     1441 gggaatcgtc gaagtcgacg aagtgcggcc cgccgtcggt ccacaacacg ttgccgaggt
     1501 ggcagtcgcc gtgcaggcgc agccggcgcg gcgtgccggc gcgttgccag gcgatctcgg
     1561 cggcgtcgag cgcctgcgcc gcgagcgcca ggtaaaccgg cgccgattcg ggcgggatgc
     1621 gttcggaggc ggcgagttcg gcgagcggct ggcgtccgta gtcgtcgacg ctgagcgccg
     1681 gccgttcggc gaatggcgcg cggccgccga ccacgtgggt gcgcgcgagg aagcggccca
     1741 tctgctcgat cgcgccgggg cgatccagct cgggcgcgcg gccgccctgg cgcgggaaca
     1801 cggcgaagcg caggccgtca gcgtggtgca gcgtgacgcc gtcgcgggcg agcggcgcga
     1861 ccactggcac ttcgtcgtcg acgagttcgg cgaggaaggc gtgctcctcg atgatctgcg
     1921 cgtcgctcca gcggccgggc cggtagaact tggcgatgac aggcggcgaa tcgtcgaggc
     1981 cgacttgata gacgcggttc tcgaagctgt tcagcgcctg cagacggccg tcggtggcga
     2041 agccgacggc gtcgagcgcg gcgagcagcg tctgcgggtc gagcgcgcca aagggttgcg
     2101 gtccgaccgc agtggcatcg ggaagtggca tcggctagcg ccggcggatg cggtcggcga
     2161 gcaccgcgcc gtcgctgccg agcgtggcgc ggacctcgac gtagtcgccg gggcgcgggg
     2221 tcttgtcgat gcgcgtgccg cggttgatgg ccagcggcag ttcgtcgagc cggtcgccgg
     2281 attcgagccg gccgtgcagg cgaaggcgtg cgccgaccgg cgcggcggcg gtcttgccgg
     2341 cgctgtcgcg cgcgccctgg acgatccagc gctcaatcag cgcgacctcg tcggcggcta
     2401 gccagggcgg tccgtcaaag ggcatgcgcg gataggcgtc gccgcgcacg cggcgcagca
     2461 gttcgctagc accggggttg cccggcacga cgcggacgcg gtcggcagta gccagcgtcg
     2521 cggcgtggct ggtcagccgg taaccctctg gcggcgggcc catcaggccg ctgtcggtgt
     2581 ggcacttagc acagcggcgg gcgaagatcg gcgcgacatc gtcgtaggtc gcgaactcgg
     2641 acttcggacg ggttggcgcc gcggctggag cggccgctgc cgagccggcg ggcataccgg
     2701 cggcgacgaa gtcgcggaac tgcgccacct cagcgtcgct aaggaagggc ggcccggtca
     2761 tcggcatgcg cggctgtttg ttgccggtga gccggcggat gagttcgctg tcgtcgggcg
     2821 cgctggccat ggcgatgggg ccgttcttgc tgccactctt aagcgcttcg aggctgtcga
     2881 gtttgagacc gagcggtgca ttgtcgccgc tgtggcacat cacgcaacgc acttgcagga
     2941 tgggcgcgat gtcggcgtac gtcgcggccc ccgcagttgt gccgaagccc agcaggccgg
     3001 cgaggagcag cgcagcgctg gtggcggtac gggcatagcg ggctgtgcgt gtcatcgtca
     3061 tcggtgtcat ggggacagta taagtaacga ctgccgcgca gcgtcgttga ccgggcgtca
     3121 gacgccgttt cggcgctgcc tgtgacgggg ctgcgtgata gactgcgccg ctccgaaacg
     3181 cccgcccaat gtccggtttc cagcccatgc agatcatcgt caacggtcag gatcgccttg
     3241 tgcagccagg cgcgaccgtc gccgatctgg tcgccgcact ggcgcttgcc gggcgccgga
     3301 tcgccgtcga actcaacggc gaaatcgtcc ccaagagttc gcacgccact aggtcgctgg
     3361 gcgagggcga tcgcgtcgag atcgtcgtcg ctgtcggagg cggctgaatg catcgagagg
     3421 aacccatgga ccagcagcaa gattttctgg atatcgccgg caagcgctac ggctcgcggc
     3481 tcttggtcgg caccggcaag taccgtgact tcgccgagac gcgcgcggcc gtcgatgcct
     3541 cgggcgccga gatcgtcacc gtcgcgatcc gccgcaccaa catcggccag gagccgggcc
     3601 agccttcgct gctcgaggta ctgccggccg accagttcac atacctgccc aataccgccg
     3661 gctgctatac ggccgacgag gcgatccgca cgctgcgact tgcgcgcgaa ctgctcgacg
     3721 gccacgatct ggtcaagctc gaagtgctcg gcgattcgca gacgctgttt ccgaacatgc
     3781 ccgagacgat cagggcggcc gacatcctgg ttcgcgacgg cttcaaggtg atggtctatt
     3841 gctctgacga cccgatccag gcgcgcatgc tcgaggagat cggctgcgtc gcggtgatgc
     3901 cgctcgcctc gctgatcggc tcgggaatgg gcatcctcaa tccgtggaac ctgcagctga
     3961 tcatcgatca agcgaaggtg ccggtgatcg tcgacgcagg tgtcggcacc gcttccgacg
     4021 cggcgatcgc gatggaactc ggctgcgacg gcgtcctgat gaacacggcc atcgccaagg
     4081 cgcggcagcc cgtcgacatg gcgcgggcga tgcgcctcgc cgtcgaagcc gggaggctcg
     4141 cctaccgtgc cggccgcatg ccgaagaaga cctatgccgc cgcacccagc tcgcccaccg
     4201 aggggctgat cggcaagtcc tgatgacggc ccgccgcgat ctcgccaccg aggcgccgtt
     4261 ctcggtcgag gacgagaagg cgcggccggt gcgcagtttc gtcctgcgcc aggggcggat
     4321 gtcggaagcg cagacgcgcg ccgcggcgac gatgatgccg accatcggcg tgccgtttcg
     4381 cgccgagccg ctcaattttg ccgcgctgtt cggccgcgtc gcgccggtgg tggtcgagat
     4441 cggattcggc atgggccata ccacggccga tatcgccgcg gcgctgcccg ctaccgattt
     4501 ccttggcgtc gaagtgcacc tgccaggcgt cggcagcctg tgcaagcgca tcgccgacga
     4561 tggcctagac aacatccgca tcgttcgcca cgacgctgta gaggtgctgg cgacgatggt
     4621 gccgccggcc tcgctggcgg gaatccacgt gttctttccc gacccctggc cgaagaagcg
     4681 ccaccacaag cgccgcctga tccagcccgg cttcgttgcg ttagccgcca gccgccttgc
     4741 gcccggcggc tacttgcact gcgccaccga ctgggaggaa tacgcgcagc agatgctgga
     4801 cgtgctctcg gccgagccgc tgctcgacaa cgaggccgat ggctatgcgc cgcgcccggc
     4861 ctaccgcccc gagactcgct tcgaggcgcg cggtctgcgg ctcggtcacg gggtctggga
     4921 tctgcgtttt attcgtcggg aggtttcaac atgagacgct ggcttgttgc ggccgtgatc
     4981 gccctgagcg tcagcgcaac cgcactggcg acgccgcggc acgcgccggt tccggggggc
     5041 gttgcctggg tcccgctgcc ggttaccggc gacgaggcgc cgaccgcgca gtttggcgat
     5101 gcgcccttgc tcgtcgtccg cgagggtgac agctggctgg cgctggtcgg tttgccgatc
     5161 gaccaggttc ccggcgaggc gacgatacgc tgggcgcagg gcgccgccgc cggagagctg
     5221 gcgatttcgg tggccgccaa ggcctatccg acgcaggaac tgcgggtcgc gccgaagtac
     5281 gtcgagcctg atccagagac gctggtgcgc atcggcaagg agcgcgcacg ggtcaacgag
     5341 atcaaggcat tgcactccct cgagcttccc gatctgggat tcgtggcacc ggccgacggg
     5401 ccgctgtcct cgcgctacgg cctgcgccgc gtcttcaacg gccagccgcg cagtccgcat
     5461 ctcgggctcg acatcgccgt gcccaaaggc acgccagtgc ggacaccgtc ggtgggtacc
     5521 gtcgtcgatg tcggcgacta ttactactgg ggtcgcttcg tcgcggtcga tcacggccgc
     5581 ggcctggtca cgctctacgc gcatctgtcg caggtcggcg tgacggtcgg gcagcgcctc
     5641 gcagcgggcg aggtgctcgg cgctgtcggt gccaccggcc gcgtcaccgg agcccacctg
     5701 cactgggcgg tgatcctcaa cggccatgcc gtcgatcccg agcttttcct gccctagcgc
     5761 cgatttcggg cgggtgcgcc gggcatgccg gcggcaaagt tgcattgagc ccctgcttgc
     5821 ggcaccattg cgcgcccgat ccgcggaacg gtgtcaacct gtgtcgaagt cggcgcgtta
     5881 atgggcgcag acgaggcgga agacgctgcc agcgtaaaca gcgctcggtc gccgtagtcg
     5941 gcaaagtgga ttttttaacc ctgatacgga gcacaacatg aaagctttca ccgtcgcact
     6001 gatggccgtt gccggcctcg ccatttccgg ccaagccgcc gctgacgaag cactggccaa
     6061 ggccaagaac tgcctgacct gccaccagga cgccatcaag atcgtcggtc cgtcctacaa
     6121 ggatgtcgcc gccaagtaca agggccagaa ggacgccgtt gcgaccctcg ccgccaagat
     6181 caaggccggt ggcaagggca actggggcga agtcccgatg ccgccgaaca acgtcaccga
     6241 cgaagaagcc aagaaactgg ccgcctgggt tctggccaag taatcgctgc agcaatcgag
     6301 aagccggctc ttgggccggc tttttttttc aaacggcgtt tgtccggggc ttgccttgac
     6361 accccatagg gctgtgcgga caatctgccg ctcgctgtcg ccggctggct aagaccggtc
     6421 gcggcggaca ccggatcacc attcacacac aaacaaggag acgatcgatg cgcaagtatc
     6481 gagttatggg aatttcgacc gcgattatgg cggcctttgc aatgctgccg acctctgctt
     6541 cggccgacac ggtcgtgaag atcggccacg tggcgccgct gaccggcccc attgctcacc
     6601 tcggcaagga caacgagaac ggtgcgcgtc tggccatcga gcatgccaat gcacagggca
     6661 tcatgatcgg tgggcagaag gtcaaattcg agctggtcgg tgaagacgac caagccgatc
     6721 cgaaaacggg caccgtcgtc gcgcagaagc tcgtcgacgc caaggtcgcc ggcgtcgtcg
     6781 gtcacctgaa ctcgggtacg acgattcccg cctcggccat ctataaccag gccggcctgc
     6841 cgcagatctc gccctcggcg accaacccca agtacacgca gcagggctac aagacggcct
     6901 tccgcgtcat ggccaacgat gtgcagcagg gctcggtaat cggtacctat gcggtgaagg
     6961 gcctcggcgc caagacgatc gcgatcatcg atgaccgcac cgcctacggc cagggtcttg
     7021 ccgacgaagt cgagaaggcc gccaaggctg ccggtgccaa ggtcgttgcc cgcgagttca
     7081 ccaacgacaa ggcgaccgat tttcgcgcca ttctcaccaa gatcaagtcg accaagccag
     7141 acgtcatctt ccacggcggc atggatgcca ctggcggtcc gatgctcaag cagatgcgtg
     7201 acctggcgat caagtcgacc ttcaccaccg gtgacggtgg ctgctcgccg gagatcatca
     7261 agcttgccgg caaggcagcc gagggcgtga tctgcacgca ggccggccta ccggtcgaca
     7321 agctgccgaa cgcgcagcgc ttcatgaccg acttcaccaa aaagtacggc cagatccaga
     7381 tctactcgcc gtactcctac gatgcggtga tggtcctgat cgatgcgatg aagcgtgcca
     7441 attcgaccga tccggccaag tacctgccga tggttgcttc gaccaatctc gacggggtga
     7501 ccggcaagat cgctttcgac gacaagggtg acgtccgcaa tggcgccatc accgtcttca
     7561 agatcgaggg cggtaagctg aacacgctca agatcgaagg cggcagcatg taattcgcct
     7621 tcgcccgcgg aggcgctagc ctctgcgggt ggcggggccg cggtggtgac gccgcggtct
     7681 ttcgtttcta gctggctctg catggccggc tcgctgtgga attgcgacga tggacatctt
     7741 cctgcagcag ctcatcaacg gcatcacgct cggcgccgtc tacgcgctgg tggcgctcgg
     7801 atacacgatg gtctacggca tcatcggcct catcaacttc gcccacggcg aggtcgtgat
     7861 gatcggcacg atggtggcgt tcaccgtcat cacggccctg gcggccagca ccggcatgcc
     7921 gccgctactg atcttctcct gtggcgccct ggcggcgatg gctgcctgca tgctgctcgg
     7981 tttcggcctt gagcgcgtcg cctatcgccc cttgcgccat gcgccacgcc tcaccccgct
     8041 gattacggcg atcggcatgt cgatcgttct gcagaacgtc gcgatgatcg tctggggtcg
     8101 caattacatc aatttcccgc agatgctcga caagcagatc tacgaggtcg gcggcgccgt
     8161 catctcgaac gtgcagatcg gcatcatcgt gctcgcgatg atgaccatgg gtggactgct
     8221 catgctcata tatcggaccc ggctcggcgc cgcgatgcgc gcgacggcac agaacccgca
     8281 tgtcgcgggg ctgatgggca tcgacatcaa ccgggtcatt tcgctggcct tcgtcatcgg
     8341 ctcgctgctc ggcgcagtgg ccggcctgat ggtcggcacc tactacgaaa tcgctcacta
     8401 ccagatgggc ttcatgctgg gcctcaaggc ctttaccgcc gccgtgcttg gcggcatcgg
     8461 caacgtcggc ggcgcgatgg ccggcgggct gctgctcggc gtgatcgagg cgctcggcgc
     8521 cggctatatc ggtgatctga ccggcggatt cctcggcagt cattatcagg acatctttgc
     8581 cttcgtcgtc ctgatctgcg tgctgatgtt ccggccgtcc ggcctgctcg gcgagcgtca
     8641 gggagaccgc gcatgagccc cgagttcatc gaacgccatc gcatgctgct gttcgtgacg
     8701 agcggcatcc tgctggcaat actgcccttc atcgtcgacg cggcggccgg gcaggcgtgg
     8761 gttcggattc tcaacttcgc gatcctctac gtcttcctcg cgctcggact caacatcgtc
     8821 gtcggcttgg ccgggctgct cgacctcgga ttcatcgcct tctacgcggt cggcgcctat
     8881 ctctatgcgc tgttcgcctc gccgcacttc gacctgcata cctcgttctt catcatgctg
     8941 cccttctgtg cagcgctcgc ctgcctgttc ggcgtcatgc tcggcgcgcc gacgctcaag
     9001 ctgcgcggcg actacctggc gatcgtcacg cttggcttcg gcgaaattat ccgcatcttc
     9061 ctcaacaacc tgaacgcgcc gatcaacgtc accaacggcg cacaggggat cacgcggatc
     9121 gacccggttt cgatcggcga gatgcaattc aacacgacga cgcacttcct tggcatcgac
     9181 ttcaccagtc cgaccaagta ctactacctt ctgttggcgc tggccttctt cgttatcctc
     9241 gtcaacatcc gtctgcatca ctcgcggatc gggcgcgcct ggcaggcaat ccgcgaggat
     9301 gaggtggcag ccaaggcgat cggcatcaac acccgcaacg tcaagctgct ggcgttcgcg
     9361 atgggagcct cattcggcgg tattgccggt gcggtcttct cggcctcgca gggcttcgtt
     9421 tcgccggaga gcttttcgct gctcgaatcg atcaccattc tggcgatggt tgtgctcggc
     9481 ggcatgggcc acatccccgg cgtgatcctc ggcgcggtgc tgttgacgat tttgcccgag
     9541 gcgctgcgct acggcatcga gccactgcag cacgcgctgt tcggcaagag ctacctcgat
     9601 cccgagagcc tgcgcatgct gatgttcgga ctggcgctgg tgctggtgat gcgttaccgc
     9661 ccggcagggc tctggccgtc gcccgagcga cgtcgtgaac tggccgagcg ggaggactga
     9721 gtcgtggcgg atcaggaact gctgctcgag gcgcgcggcg tcgcgaagca tttcggtggc
     9781 gtcaaagcct tgcaggatgt gtcgctgacc attcgccgcg gcgaaatcta cggactgatc
     9841 ggccccaacg gcgccgacaa gaccaccttc ttcaactgcc tgaccgggct ttaccgcaac
     9901 gagcacggcg gcttcgtctt cgacggcgag ccgctggaag ccgatgcgcc gcatctggcg
     9961 gcgatccgag gcatcgctcg gaccttccag aacatccgcc tgttcggcaa catgacggcg
    10021 ctcgagaacg tcatggtcgg ccgccacctg cgcacgcgtg ccaatgtttt cggggcaatc
    10081 tttcataccg ccgccacact ggctgaggag cgggcgattt cgcgccgcgc gctcgagttg
    10141 atgcactacg tcggcatcgc cgaccgggcc ggcgactttg cgaagaacct gtcctacggt
    10201 gatcagcgcc gtctggaaat cgcccgggcg ctcgccaccg acccgaagct gctggcgctc
    10261 gatgagccgg ccgcgggcat gaacggggcg gaaaccgaag atctgcgcgt gctgatcgag
    10321 ggcatccgcc gggacggtgt gaccgtgctg ttgatcgagc acgacgtcaa gctggtcatg
    10381 gggctgtgcg atcgcgttgc cgtccttgac tacggcgaga agattgccga ggacgttcct
    10441 gcggtggtcc agcgcgatcc gcgcgtgatc gaggcctatc tcggcggttc gccggactga
    10501 tcgaacggag agagcggacg atgctcgaag tcacgggatt gcaggtcgcc tacggtgcca
    10561 ttcaggccgt ccgcggcatc accttccaca tcgaaaaggg cgagatggtc gcgctgatcg
    10621 gcgccaacgg cgccggcaag acgaccacgc tgaaggcgct ggcgcgcatg cacgacgttg
    10681 ccggcggacg catcagctac tgcggcgaag acattacgcg cattcccccg caccagttgg
    10741 tgcgcaaggg gattgccctg gttcccgagg ggcgtggcat gttcccgcgc ctaactgtgc
    10801 acgagaacct gatgatgggc gccttctcgc gaaatgattc gcgcgagatc gctgccgaca
    10861 tcgaccaggc cttcgagcgc tttccgcgtc tgcgcgagcg ttcgcagcag ctcgccggca
    10921 cgctctccgg cggcgagcag cagatgctgg cgatcggccg tgcactgatg agccggccga
    10981 agctcctgct gctcgacgag ccctcgatgg gactggcacc gatcatggtg cagaagatct
    11041 tcgaggtcgt taaggaggtc gccaaggccg gaatgacgat tctgctcgtc gagcagaacg
    11101 ccagacttgc cctgcaggtc agccgtcgcg cttacgtgat ggagtcgggc gagatcacca
    11161 tcagcggcga gtcagtcaag ctgcttgacg acccgaaggt gcgcgccgcg tatctgggcg
    11221 ccgagtagcg ccagccggta gctcgcgcgc agcagttccc acggcaaata ccaaatgtct
    11281 tccggttgtc gaatcgcgcc cggaggcggg gctcagaccg ccatcgtcgc cgagaagtcg
    11341 atcgcctgaa gcagcgccgc gttcaccttg gctgccgtga gcccgagtgc ctgcgcccgc
    11401 gttgcgatgg ctttcgcgtc gcccacctcg acggcaagcg ccagatcgag aaagggtgcg
    11461 tagctgcccg cacgctgcag caacgcctcg ctgatcggtc ccggcagatt catcgcgtct
    11521 agcgccttgt ccatcgacac cccgagcagc acgtcgagca gcgagaaggc gccggtgatg
    11581 aacaggttgt cgagttcgcc cttgtcgacg aggccttggc cgagcagctc catcagccgt
    11641 gcgcggacca gcgccgcatg catctgcgct ggcgccagcg ggtccttgct cgccgttacc
    11701 agcagcagcg acagccactt gttgagcttg tcgtaaccga ggatcgtcac cgcatggcgg
    11761 aaggattgaa tctcgcagga cagaccgaag ccggccgagt tgatgtagcg cagcagcttg
    11821 taggagagcg ctacgtcctg cttgagcgcg gtttcgatct gcggcacgtc ggcgttgttg
    11881 cgcactagat tgagtacgcg gacgatctgc gcctgcgctg gcgccagcgt cttcgccatc
    11941 gtcgacgagg gctgcttgca gaaccatcca gacgcggccg tgatgcccga atcgatgcag
    12001 gccttgaagt cggtcggcgt cgcgacgtta agcgtgatcg gcgtgccgag ctggcgcagc
    12061 ttgacggtga gcgcctgcat ttgcgcaatc ggtacctgcg cgccgtcgag cgcgaaaaag
    12121 cggaagctca agttggcgcc gttggcgttg ccgacgtcgg ggtcgacatc gatgcacagt
    12181 ggcgcggcat tgtcgcgcag cgcctgggca gtggcgacgc cggcttcacc ggcgaggtcg
    12241 ctgccgcgga tttcgaggat tacattttcg ggcagcagcc agtcgaacag cgcgggatcg
    12301 acgcgcgcgt tgccgagcga gagaaagaaa gagaccgact gattcatcgg ccagaattct
    12361 tcgacttggc gcagcgctgc aaccgctgat ctggagtcgg cggcatggac gatcaggcgg
    12421 ttcgcggtga gcttgccgtt cttgccaagg atgggctgat gcgagaagta ggtgtcggac
    12481 atcaagagct ctcagttccg tgggcaaagg tgaaggcgtc ggcgaagaag cgctcggccg
    12541 gcaggccgca gcgcgtcgta aagtcctcgc gcgcggcact gatcatcgcc ggcgcgccgc
    12601 agatgtagac gtcgctggca gcgagctggc cgctgtcgcc gaggtcgtcg ataacggcct
    12661 cgtggaccag gccgcggcgg ccaacccagt cgtctccggc ggccggctcg gacaacacgg
    12721 gcacgaagtg cagttgcgcg tgctcgcggg cccagcgttc ggcgagcgcg atagcgtaga
    12781 gcccggcacg gtcgcgggcg ccccaataca gccgaattgg ccgcgtgcag ccagtatgca
    12841 gcgcgtgttc gatgatcgcc ttgatcgggg cgaagcctgt gccgccggcg acgcagacga
    12901 tcggccgctc ggatttttcg tcgagcgtga agctgccgag cgggccgtgt acgcgcagga
    12961 tgtcgcgcac tttcatcgtt gcgaacacct ggtcggtgaa ttcgccgccg ggaacgtggc
    13021 ggacatgcaa ttcgatcagc gcatcgtcgt gcggcggatt ggccatcgag aaggcgcggc
    13081 gctcgccgtt cttgagcagg atctcaaggt actggccggc gaggaactgt aggcgttcgg
    13141 tggccgggat gcgcagcgcg atgcgcatga cgtcagacgc cagccggtcc atcgcctcga
    13201 tgcgcgccgg aaaggtcttg gccgggatcg cactggcggc gacctcgcgg cagacgatct
    13261 cgatatcgct ctcggcgcgc gcgcagcaga gcagcgtgaa gccctgcgct ttttcatcgt
    13321 cggtgagcgt cgccgggtcg gcgccgtcgt gatcgactcg cccctcggtc accagcgtct
    13381 tgcaggcgcc gcaggcaccg ttgcggcagc cataggggag gttgattccc tggcgcaatc
    13441 cggcttcgag gatgctttcg tcggccatag tggtaaaggc cagatcgctg tccctgacga
    13501 cgacacggta actcatgatt gcaatgcttc tccgctagaa tgcgccgatg attcgtgtcc
    13561 tgatcgtcgg cgccggggat gtcgcgcgac gactccatcc cttgctggcc cgccgcttcc
    13621 gcgtattcgt tttgctgcga aatgccgaca aggctggcgc ctggcgggcg gccggcgcga
    13681 cgccgatcgc tggcgacctc gatgaccggc gcagtctcga tcgggcagcg gcgctcgcgc
    13741 agtggatcgt ccatctcgct ccgccaccat caggcggtgc cgacgaccct cgtaccgcgc
    13801 acctgctcgc ggcccttcgc aggcgggcga gtctagcaca gcgtcttgtc tacatcagta
    13861 ccagcggggt ctacggagac tgcggcgggg cgcgtatcga cgagtcgaaa accccgtcgc
    13921 caaagacccc gcgcgggcga cgtcggctgg ctgccgagcg ccgtgttcgc agctacgcaa
    13981 aaagccgacg cgcgagcatc ctgcgggtac ccggcatcta cgccgccgac cgcctgccgc
    14041 tggcgcgcct gcagtcgggg acgccggctc tgcaggccaa cgatgacgtg ttcaccaatc
    14101 acatccacgc cgacgacctc gccgcatcgg tgcggcaggc cctgctctac gggcgaagcg
    14161 ggcgtgttta tcacgtggtc gacgacagcg agcagcgcat gggcgattac ttcgacgccg
    14221 tggctgatgc cttcggcctc gcccgcccgc cgcgcatctc gcgtgccgat gccgcgcgac
    14281 agattgaccc gggcatgctc tcgtttatgt ccgaatcgcg gcgactggac aattcacgca
    14341 tgaagcgcga actgcgcctg cgcctgcgct accccaatac cgcagcggga atcgccgccg
    14401 ctctcgaccc tgaaagactc ccatgctgac ccttaaggcg ctgcacctca tcttcgttac
    14461 cagctggttt gccgggcttt tctacctgcc gcgtatcttc gtcaatctcg cactggtccc
    14521 ggccggcagt gtcgccgagc gcgaccgcct gctgctgatg gccggcaagc tttatcgctt
    14581 catgctgccg ctcgccgtgc tggcactggt tttcggcatc tggctgtggc tcgcctacgg
    14641 ctacggcggc ggctggctgc acgccaagct ggcgctggtc gtcgtgctgg tgatctacca
    14701 cgcggtctgc gggcgccacc tcaagcagtt tatcgccggc aacaacacgc gctcgcacgt
    14761 ttggtaccgc tggttcaacg agctgccggt actggtgctg gcagtcgtgg tcttcctcgt
    14821 cgtgctcaag cccttctgag ccgcgcgatg gaacgctttt ttgccagctg cccgcgcggg
    14881 ctcgaagcgc tgcttgccga ggatctggtc ggctggggtg cgagcgaggt ccgcgccgag
    14941 ggtggcggtg tcgccttcgc cggcgactgg ccggtgtgct ggcgtgccaa cctgcattca
    15001 cggctggcga cgcggattct ctgccaggtc gccgagggcg cctaccagaa ggaagacgac
    15061 atctaccggc tggcgcgtgc cgtcgattgg gccgcgcgct tttcggtcga ccgctcgatc
    15121 cgtgttgcga tcacggcggt tcgttcgccg ctgaagagca tcgacttcac cacgctgcgg
    15181 atcaaggacg cggtctgcga tcgctttcgc caggactgcg gtcgccgccc cgacgtcgac
    15241 acgcgggcgc ccgacgtccg aatccacgcc ttcctcaacg ccgagcgggc aacgctctac
    15301 ctcgatacct caggcgagcc gctctaccac cgtggcctgc ggcagaagac cgtcgatgcg
    15361 ccgctcaagg aaaacctcgc ggccggcatc ctgcgcctca ccggctggca gccgggcgtc
    15421 gccttgttcg atccga
//
LOCUS       DATDAA010000451         2246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_33029,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000451 DATDAA010000000
VERSION     DATDAA010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33029"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VIS73_13610"
     CDS             complement(<1..1026)
                     /locus_tag="VIS73_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY5764240.1"
                     /translation="MSAANPAIQAPAADSHDGHHEHQAPYGWRRWLFTTNHKDIGTMY
                     LTFALIMFFVGGAMILLVRAELFQPGMQLIQPEFFNQLIGVHALVMIFAALMPAATGF
                     ANWQIPLMIGAPDMALPRLNNWGFWILPPAATLLTLPFILALFGIGDGAIATGWTFYA
                     PLSVQGGIGVDFAIFAVHLLGISSIMGSINVIVTLFNMRAPGMSLMKMPVFCWGWLIT
                     AFLLIATIPVLAGAVTMLLTDRHFGTHFFDAAGGGDPILFQHLFWFFGHPEVYILLLP
                     IWGLVPHVLATFSRKPMYGYKGQVYGFIAIGLLSVVVWAHHFFTAGLPVPALLYFMYS
                     TMAISIPL"
     gene            complement(1037..>2246)
                     /locus_tag="VIS73_13615"
     CDS             complement(1037..>2246)
                     /locus_tag="VIS73_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY5764241.1"
                     /translation="NAEAKGEHYLLEVDNPLVLPVGKKVRVLLTSTDVIHTWWVPQFG
                     VKRDAIPGFLRETWVKIEQPGTYRGQCAELCGKGHGFMPVVVEALPEPEFNEWLARKE
                     AQLAELAAGADRAWSKAELIEQGATVYAQQCAVCHGQEGQGIPGAFPALTGSKLVTGP
                     LLTADGQVIAGSHVDRVLNGKPGTAMQAFRNTLSPVDLAAVITYERNAIGNAVGDFIQ
                     PAQIRALMGGAPSAASAPAPAAEAAAAAAPVPAAVIPDPQADVKKPAAAPPAATPPPT
                     PSAKPASAAAKAPAKLGKDELMATGKSVYTARCLACHGAEGKGIPGAFPALSGSPAVN
                     GPLLGPGGKLIAGGHVDRVLNGKMGTPMQAFRDVLSDTELAAVITYERNALGNSMGDL
                     IQPAQIQALR"
BASE COUNT          379 a          704 c          800 g          363 t
ORIGIN      
        1 cagcgggatc gagatcgcca tcgtcgaata catgaaatag agcagcgccg ggaccggcag
       61 gccggcggtg aagaaatggt gcgcccagac cacgaccgaa agcaggccga tggcgatgaa
      121 gccgtacacc tgacccttgt acccgtacat tggcttgcgg gagaaggtcg ccagcacgtg
      181 cggaaccaga ccccagatcg gcagcagcag gatgtagacc tcagggtgac cgaagaacca
      241 gaacaggtgc tggaagagga tcgggtcgcc gccgccggcg gcatcgaaga agtgcgtgcc
      301 gaagtggcgg tcggtgagca gcatggtcac cgcgccggcc agcaccggga tcgtcgcgat
      361 cagcagaaag gccgtgatca gccagcccca gcagaagacc ggcatcttca tcaggctcat
      421 gcccggcgcg cgcatgttga agagcgtgac gatgacgttg atcgaaccca tgatcgagct
      481 gatgccgagc aggtgcacgg caaagatggc gaagtcgacg ccgatccccc cctgcaccga
      541 gagcggcgcg tagaaggtcc atcccgtggc gatcgcaccg tcgccgatgc cgaacagcgc
      601 gaggatgaag ggcagcgtca agagcgtcgc cgccggcggc aggatccaga agccccagtt
      661 gttcagacgc ggcagcgcca tgtcgggggc gccgatcatc agcggaatct gccagttggc
      721 aaagccggtc gccgccggca tcagcgccgc gaagatcatc accagcgcgt ggacgccgat
      781 cagctgattg aagaactcgg gctggatcag ctgcatgccc ggttggaaca gctcggcacg
      841 caccagcagg atcatcgcgc cgccgacgaa gaacatgatc agcgcgaagg tcaggtacat
      901 cgtgccgatg tccttgtggt tggtcgtgaa cagccagcgg cgccagccgt agggcgcctg
      961 gtgttcgtga tggccgtcgt gcgaatcggc ggccggggct tgaatggcgg ggtttgcggc
     1021 gctcatcggg ttctcctcag cgcagtgcct ggatttgcgc gggctggatc aggtcgccca
     1081 tgctgttgcc gagcgcgttg cgctcgtaag tgatgacggc ggcgagttcg gtgtccgaca
     1141 gcacgtcgcg gaaggcctgc attggcgttc ccatcttgcc gttgaggacg cggtcgacgt
     1201 ggccgccggc gatcagtttg ccgcccggcc cgagcagcgg gccgttgacg gcgggcgagc
     1261 cgctcagcgc cggaaaggcg ccggggattc ccttgccctc ggcgccgtgg caggcgaggc
     1321 agcgggcggt atagaccgac ttgccggtcg ccatcaactc atccttgccg agtttggccg
     1381 gcgccttggc ggccgccgag gctggtttcg cagacggtgt cggcggcggc gtcgccgcgg
     1441 gaggcgcggc tgccggcttt ttcacgtcgg cttgtgggtc cggaattacc gctgcgggca
     1501 caggcgcagc cgccgctgcc gcttccgcag caggcgccgg ggcgctggcc gcgctcggcg
     1561 ccccgcccat cagcgcgcgg atctgcgccg gctggatgaa atcgccgacg gcgttgccga
     1621 tggcgttgcg ttcgtaagtg atcactgccg ccaggtcgac cggcgacagc gtattgcgga
     1681 aggcctgcat cgccgtgccg ggcttgccgt taaggacgcg atcgacgtgg ctgccggcaa
     1741 tcacctggcc atcggcggtg agcagcgggc cggtgacgag cttgctgccg gtgagtgccg
     1801 ggaatgcgcc ggggatgccc tggccctcct ggccgtgaca gaccgcgcat tgctgcgcat
     1861 agaccgttgc gccctgttcg atcagttctg ccttgctcca ggcgcggtcg gcgccggcgg
     1921 cgagctcggc gagctgagct tccttcctgg ccagccattc gttgaattcg ggttcgggaa
     1981 gcgcctcgac gaccaccggc atgaagccgt gccccttgcc gcagagttcg gcgcattggc
     2041 cgcggtaggt gcccggctgc tcgatcttta cccaggtctc gcgcaggaag ccggggatcg
     2101 catcgcgctt gacgccgaac tgcggcaccc accaggtgtg gatgacgtcg gtcgaggtca
     2161 gcaggacgcg gaccttcttg ccgaccggca ggaccagcgg attgtcgacc tcgagcagat
     2221 agtgctcgcc cttggcttcg gcattg
//
LOCUS       DATDAA010000452         9855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_313442,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000452 DATDAA010000000
VERSION     DATDAA010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313442"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2424)
                     /gene="carB"
                     /locus_tag="VIS73_13620"
     CDS             complement(<1..2424)
                     /gene="carB"
                     /locus_tag="VIS73_13620"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY5764242.1"
                     /translation="MPKRTDIKSILVIGAGPIVIGQACEFDYSGAQACKALKQEGFRV
                     ILINSNPATIMTDPDTAHVTYIEPITWQAVERIIDKERPDALLPTMGGQTALNCALDL
                     AKHGVLQKYGVEMIGASRDAIDKAEDREKFKNAMTKIGLGSARSAIAHSMEEAHQVQA
                     GIGFPVIIRPSFTLGGTGGGIAYNTEEFVEICKRGLEASPTNELLIEESLIGWKEFEM
                     EVVRDRKDNCIIVCAIENLDPMGVHTGDSITVAPAQTLTDKEYQILRNASIAVLREIG
                     VDTGGSNVQFAINPDDGRMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LANEITGGATPASFEPAIDYVVTKVPRFAFEKFPQADSRLTTQMKSVGEVMAIGRTFQ
                     ESFQKALRGLEVGVDGMNQKTTDRERIARELGHPGPERIWYVGDAFENGFSVEEVHAL
                     THIDRWFLVQIEEIIRLEMALDDMALSAIDEATMRFLKRKGFSDRRMAHLLHCSEAEV
                     RQHRIQLGVRPVYKRVDTCAAEFSTSTAYMYSTYEEECESVPTDRKKIMVLGGGPNRI
                     GQGIEFDYCCVHAALALREDGYETIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLEIV
                     AIEKPVGVIVQYGGQTPLKLARDLEKNGVPIIGTSPDMIDAAEDRERFQKLLNDLGLK
                     QPPNRTARTESQALSLAEEIGYPLVVRPSYVLGGRAMEIVHQPADLERYMREAVKVSN
                     DSPVLLDRFLNDACEVDVDALSDGEEVIIGGVMEHIEQAGVHSGDSACSLPPYSLAAP
                     LQEELRRQTV"
     gene            complement(2476..3618)
                     /gene="carA"
                     /locus_tag="VIS73_13625"
     CDS             complement(2476..3618)
                     /gene="carA"
                     /locus_tag="VIS73_13625"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY5764243.1"
                     /translation="MPSFNPAVLVLADGSVFHGHSIGALGQTSGEVVFNTAMSGYQEI
                     LTDPSYCRQIVTLTYPHIGNVGCNAGDFESDRNYAAGLIIRDLPAVHENWRAEQSLGD
                     YLKSHGVVAIAEIDTRRLTRILREKGAQAGCIVAAERLPDDAIAQAQAAARGFPGLAG
                     MDLAKEVTVSAGYRWQEGEWTLSGFAEPPASKFKVVAYDFGVKRNILRMLASRGCDVH
                     VVPATTSAAEVLAMKPDGVFLSNGPGDPEPCAYAISAIQEILDKRIPVFGICLGHQLL
                     GLASGARTVKMKFGHHGANHPVKDIDSGQVLITSQNHGFAVDAETLPANLRATHASLF
                     DGSLQGIARTDVPAFSFQGHPEASPGPHDVAYLFDRFTGLMEQGRN"
     gene            complement(3869..4675)
                     /gene="dapB"
                     /locus_tag="VIS73_13630"
     CDS             complement(3869..4675)
                     /gene="dapB"
                     /locus_tag="VIS73_13630"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY5764244.1"
                     /translation="MDMVKVAIAGASGRMGRMLIEAALRDPGIELVGALDRPGSPVIG
                     KTAGELVGMECAVPVVADVATILQRADCLIDFTRPEATLAHLAACKLAGKALVIGTTG
                     LDADARATIRQGSEHVPVVYAPNMAVGVNLVFKLLDTAARVLAQGYDIEVVEAHHRHK
                     VDSPSGTALRMGEVLADALGRDLKACAIYGREGVTGERDPSTIGFATVRGGDIVGDHT
                     VMFCGTGERVEISHKAGSRMPYALGSMRAAKFLAGRRSGLFDMQDVLGLR"
     gene            complement(4687..5127)
                     /locus_tag="VIS73_13635"
     CDS             complement(4687..5127)
                     /locus_tag="VIS73_13635"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEY5764245.1"
                     /translation="MTCRTPCQILARRVASLLIPLAVGACGWLAPYRIDIQQGNVVEA
                     SQIAQLRAGLTREQVRFVLGTPLITDVFHENRWDYVYYVDRRSTGEREQGRASLFFGS
                     DGLLERWVADVAPSRASQRQNRVIDITGGAAGSSEPAAVPAENG"
     gene            5212..5676
                     /gene="fur"
                     /locus_tag="VIS73_13640"
     CDS             5212..5676
                     /gene="fur"
                     /locus_tag="VIS73_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEY5764246.1"
                     /translation="MSNADELKNIGLKATTPRLRVLELFQHSEQRHMTAEDVYKALTL
                     EGFDIGLATVYRVLTQFEQAGLLNRHHFESDRSVFEVNEGTHHDHLVCLQCGRVEEFF
                     DPVIEKRQAEIAAQRGFTIREHALYLYVDCQRTDCEHHPAAGADAKSGGPAE"
     gene            5749..6303
                     /locus_tag="VIS73_13645"
     CDS             5749..6303
                     /locus_tag="VIS73_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEY5764247.1"
                     /translation="MDAFLTSTLLVAIAEIGDKTQLLSFVLAARLRAPLAIICGIFVA
                     TVANHALAGSVGAWVATLLAPEVLRWLVGLGFIAFGLWALKPDTLEDEPSASRWGPFI
                     TATVAFFIAEMGDKTQLATVALAARFDALAPVVVGTTLGMMIANVPAVLVGSKLADRL
                     PLGIIRWVAAGGFVATGLWTLWPR"
     gene            complement(6315..6875)
                     /locus_tag="VIS73_13650"
     CDS             complement(6315..6875)
                     /locus_tag="VIS73_13650"
                     /inference="COORDINATES: protein motif:HMM:NF023540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
                     /protein_id="HEY5764248.1"
                     /translation="MEIGSATFARIGPLAPQERGGLRADARQTEATGQTPAEARQIAE
                     LRRIDQRVRAHEFAHLSAGAGVVSGGARYQYTVGPDGQRYATGGEVPIDGSPGRTPEE
                     TLAKAQKIRRAALAPVDPSNTDRRVAAQAAAMEAEARAQIQTLAREEATAGSEVSQTS
                     DDDGRLSLYRAVETGENAPATFQASA"
     gene            complement(6914..8584)
                     /gene="recN"
                     /locus_tag="VIS73_13655"
     CDS             complement(6914..8584)
                     /gene="recN"
                     /locus_tag="VIS73_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY5764249.1"
                     /translation="MLKQLTIRDFVLVDRLELPFESGFGVLTGETGAGKSILVDALAF
                     VLGERADTGLIRAGAERAEVSAEFEVAGNDMVRRVLADQELGDEDGVLLLRRVVDAGG
                     RSRAWLNGTPVTLQQLREITDLLVDIHGQHAHQSLLRADAQRDLLDAYGDLVELAAEV
                     ADTYRRWRDAQAHWQASLTDGEALRRQRESLAWEVDELRALAFSPDEWQSLNAEQAKL
                     ANAVALLEGSTYCVQALGEAEYSCSSQLSAVAARLDELAAYDASLAELGALVRSAEAE
                     VGEALAGLRRYGERVDLDPSRLAEVDQRLEAILGTARKFRIQANDLGDALVERERQLA
                     ALGDAANPEKLAEASRAAEARFRTLAERLGAARRTVATQLGKEVSAQMGTLAFGGGSF
                     EVTLTPVEEGGSFGLERVEFRVGGLAGSEMKPLARVASGGELSRISLALQVVASRAAK
                     VPTLVFDEVDVGIGGGVAEVVGRLMRSLGRNRQVLCVTHLPQVAACADWQWRVSKARR
                     GDAFVSAVERLSAASREEEIARMLGGVEITPVSRQHAREMLAVVDAID"
     gene            complement(8601..9503)
                     /locus_tag="VIS73_13660"
     CDS             complement(8601..9503)
                     /locus_tag="VIS73_13660"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEY5764250.1"
                     /translation="MRSEQIPRTVALIGKFGSPEIGEALVALGRFLRERGVTVYVEEE
                     TAKRALPQAVQEFVPAAFAAIGAQADVAIVLGGDGTMLNAARQLARFGVPLVGVNQGH
                     LGFMTDIARSEMLTTMEDLLDGRFVPEVRMMLDAEVIRDGREITANLALNDIVIDKGA
                     IGRLIEIDLTIDGEFIYTLRADGLIVTTPTGSTAYALSANGPVMHPSLQAIGLVPLCP
                     HALTNRPVIVGEQSELELRVTHGADPRVHFDGQVTCDLRPGDSVRIRRSDYAIRFLHP
                     PAYSYFAMLRQKLHWSERPVGNKG"
     gene            9587..>9855
                     /locus_tag="VIS73_13665"
     CDS             9587..>9855
                     /locus_tag="VIS73_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY5764251.1"
                     /translation="MLDERARILLKTLVERYITDGQPVGSRALSKVSGLDLSAATVRN
                     VMADLEEMGFIASPHTSAGRVPTARGYRFFVDSLLTVQALEREKL"
BASE COUNT         1738 a         3279 c         3049 g         1789 t
ORIGIN      
        1 gacggtctgc cggcgcaatt cctcctgcag cggcgcggcc agcgagtacg gcggcagcga
       61 gcacgccgag tcgcccgaat ggactccggc ctgttcgatg tgttccatga caccgccgat
      121 gatgacctct tcgccatccg acagcgcgtc aacatcgacc tcgcaggcat cgttgaggaa
      181 gcggtcgagc agcacgggag aatcatttga aaccttcacc gcctctcgca tgtagcgctc
      241 gagatcggcc ggctgatgga cgatttccat tgcccggcca ccaagcacgt acgagggccg
      301 aaccaccagc gggtagccga tctcctctgc cagcgagagc gcttggcttt cggtgcgcgc
      361 cgtgcggttg ggcggctgct tcaaaccgag atcattgagc agtttctgga agcgctcccg
      421 gtcttcggct gcgtcgatca tgtcggggct ggtgccgatg atcggaacgc cgttcttttc
      481 caggtcgcgt gcgagcttga gcggcgtctg cccaccgtac tgaacgatca cgccaaccgg
      541 cttttcgatg gcaacgattt cgagcacgtc ctctagcgtc agcggctcga agtacaggcg
      601 atccgaggtg tcgtagtcgg tcgacacggt ctccggattg cagttaacca tgatggtttc
      661 gtagccgtct tcacgcaggg caagtgccgc gtgaacacaa cagtagtcga actcgatgcc
      721 ctgcccgatg cggttcgggc cgccgccaag gaccataatc ttcttgcggt cggtcggtac
      781 cgattcgcac tcttcctcgt aggtcgagta catgtaggca gtggacgtcg agaattcggc
      841 ggcgcaggta tcgacccgct tgtagacagg gcgaacgccg agttggatgc ggtgctgccg
      901 gacttcggcc tcgctgcagt gcagaaggtg cgccatacgg cggtcggaga aacccttgcg
      961 cttgaggaaa cgcatcgtcg cctcgtcgat ggccgacagg gccatgtcgt cgagtgccat
     1021 ctccagccgg atgatctcct cgatctgcac gaggaaccag cggtcgatgt gggttagcgc
     1081 gtgaacctcc tcgacgctga agccgttctc gaacgcatca ccgacgtacc agatccgctc
     1141 gggtcccggg tgtcccagtt cgcgcgcgat gcgctcgcgg tcggtagtct tctggttcat
     1201 gccatcgaca ccgacctcaa gcccgcgcag cgccttctgg aaggattcct ggaaggtgcg
     1261 accgatcgcc atgacctcgc cgaccgactt catctgcgtt gtcagccgtg agtcggcctg
     1321 cggaaacttc tcgaaggcaa agcgcggcac cttggtgacg acatagtcga tcgctggctc
     1381 gaacgacgcc ggcgtcgcgc cgccggtaat ctcgttggcc agttcgtcga gcgtgtagcc
     1441 aactgccagt ttcgccgcga ccttggcaat cgggaaacca gtggccttgg aggccagcgc
     1501 tgacgaacgc gacacgcgcg ggttcatctc gatgacaatc atccgaccgt cgtcggggtt
     1561 gattgcgaac tgaacgttcg aaccgccggt gtcgacaccg atctcgcgca gcacggcaat
     1621 cgaggcgttg cgcaggatct ggtattcctt gtcggtcagc gtctgcgccg gggcgacggt
     1681 gatcgaatcg ccggtatgca cgcccatcgg gtcgaggttc tcgatcgcgc agacgatgat
     1741 gcagttgtcc ttgcgatcgc ggaccacctc catctcgaac tccttccagc cgatcaggga
     1801 ctcctcgatc aggagttcat tggtcgggct ggcctcgaga ccgcgcttgc atatctcgac
     1861 gaattcctca gtgttgtagg cgatcccgcc gccggtgccg ccgagcgtga aggacgggcg
     1921 aatgatgacc ggaaagccaa tgcccgcctg cacctggtgc gcctcttcca tgctgtgcgc
     1981 gatcgccgag cgcgccgaac cgagaccgat cttggtcatc gcattcttga acttctcgcg
     2041 gtcctcggcc ttgtcgattg catcgcgcga agcgccgatc atctcgacgc catacttctg
     2101 gaggacgccg tgcttggcca gatcgagcgc gcagttgagc gcggtctgcc cacccatcgt
     2161 cggtagcagc gcgtcaggcc gctccttgtc gatgatccgc tcgacggcct gccaggtgat
     2221 cggttcgatg taggtcacat gcgccgtatc cgggtcggtc atgatcgtcg ccggattcga
     2281 attgatgagg atgacgcgga agccttcctg cttcagcgcc ttgcacgcct gggcacccga
     2341 ataatcgaac tcgcaggcct ggccgatgac gatcggcccg gcgccaatga ccagaatgct
     2401 ttttatgtcg gtacgttttg gcatcgctat gtctctgttc gggccagcca gaaggctggc
     2461 caccctgctg ctggttcagt tgcggccctg ttccatcagg ccggtaaagc ggtcgaagag
     2521 gtaggccacg tcatgcgggc ccggactcgc ctcggggtgc ccctggaacg aaaaggcggg
     2581 cacgtcggta cgcgcaatcc cctgcaggct gccgtcgaac agcgaggcgt gggtcgcccg
     2641 caggttggcc ggcagcgttt cggcatcaac cgcaaagcca tggttctggc tggtgatcag
     2701 gacctgcccg gaatcgatat ccttgactgg atggttggca ccgtgatgac cgaatttcat
     2761 cttcacggtc cgcgcgccgg atgccagccc gagtaactgg tggccgaggc aaatgccgaa
     2821 taccgggata cgtttgtcga ggatttcctg aatcgccgag atcgcgtagg cgcaaggctc
     2881 cgggtcacct ggaccattcg acagaaaaac gccatcgggt ttcatcgcca gcacttccgc
     2941 cgcgctggtc gtcgccggga caacatggac atcgcagccg cgcgaagcaa gcatgcgcag
     3001 gatgttgcgc ttgacgccaa agtcgtaggc aaccaccttg aacttcgatg ccggcggctc
     3061 ggcgaacccg gacagggtcc actcgccctc ctgccagcgg tatccggcgg ataccgtcac
     3121 ttccttggcg agatccattc cggccaggcc ggggaagcca cgtgctgccg cctgcgcctg
     3181 ggcaatcgcg tcgtctggca atcgctcggc ggcgacgatg caaccggcct gcgcaccctt
     3241 ctcgcgaagg attctcgtca ggcgtcgcgt atcgatttcg gcaatcgcga ccacgccatg
     3301 gctcttgagg tagtccccca gcgattgctc ggcacgccag ttctcatgga ccgccggcaa
     3361 gtcgcggatg atcaggcccg cagcgtagtt gcggtcggat tcgaaatcgc cggcgttaca
     3421 gccgacattg ccgatatggg gatatgtcag cgtgacgatc tgccggcaat acgacggatc
     3481 ggtcaggatt tcctgatagc ccgacatcgc ggtgttgaag acgacttcgc cgctcgtctg
     3541 accgagcgca ccaatcgagt ggccgtgaaa gaccgaaccg tcggcaagca ccagaacggc
     3601 cgggttgaaa cttggaatga gcgacacaaa cgactcctga acgcggcctg ggtccccggc
     3661 cttggcgccg ggaccagtca ggggatggca gatgtgcgaa gcgggagagg tcgtgacctc
     3721 tcccgcttcg aactgcaagc tccgaatact ggccgcgatt ataccggtga cgggcactgc
     3781 ccggcaacgt gcgccgcgtc ctaatcgttt ccaaaccgca aaccgttccc accatctgag
     3841 cgcggcgaac gagtgatctc ccttgccgct agcgaagacc caatacgtcc tgcatgtcga
     3901 aaagccccga acgtcgtccg gcgaggaact tcgccgcacg cattgatccg agcgcgtaag
     3961 gcatccggct gccggccttg tggctgatct cgacccgctc gccggttccg caaaacatca
     4021 ccgtgtggtc gccgacgatg tcgccaccac gaaccgtcgc aaagccgatc gtcgatggat
     4081 ctcgctcgcc ggtgacaccc tcgcgcccgt agattgcgca ggccttgagg tcgcggccaa
     4141 gggcatcagc cagcacttcg cccatgcgca gcgcggtacc ggaaggcgaa tcgaccttgt
     4201 gccggtgatg ggcttcgacg acttcgatgt cgtagccctg cgcgagaacc cgcgccgccg
     4261 tgtcgagcag cttgaacacc aagtttacgc cgactgccat gttcggcgca taaacgaccg
     4321 ggacatgctc ggagccctgg cgaatcgtcg cccgggcgtc cgcatcgagg ccagtcgtcc
     4381 caatcaccag cgccttgccg gcgagcttgc acgccgcaag gtgggctagc gtcgcttccg
     4441 ggcgggtgaa gtcgatcagg cagtcggcgc gctgcaggat ggtcgcaaca tcggccacaa
     4501 ccggcacggc acactccatg ccgaccaact cgccggctgt cttgccgatt accggcgagc
     4561 cggggcgatc gagcgcgccg accagttcga ttccgggatc gcgcagcgcc gcctcgatca
     4621 gcattcgtcc catccggccg cttgcgccgg caatcgccac cttcaccatg tcgattccct
     4681 tatcccttat ccgttctctg ccgggacggc cgcgggctca cttgagcctg cggcgccgcc
     4741 ggtgatgtcg atcacccgat tctgccgctg actagctcga gacggcgcaa cgtcggccac
     4801 ccatcgttca agcaagccat cgctgccgaa gaagagactg gcgcgcccct gctcgcgttc
     4861 gccggtgctg cgcctatcga cgtagtagac gtagtcccag cggttctcat ggaacacatc
     4921 ggtaatcagc ggcgtaccga ggacgaagcg gacctgctcg cgcgtcagcc cggcacgcaa
     4981 ctgggcgatc tgcgaggctt caacgacgtt gccttgctgg atgtcgatac ggtacggggc
     5041 cagccagcca caggcgccga cggcgagcgg gatcaacagg ctggcaacgc gcctggcaag
     5101 aatttggcac ggggtgcggc aggtcatggg gatcgggaaa tttcgggttt tcgctggagg
     5161 gcactatgat agccgatccc caacaaccgc cgtgccgcca acccagcatc catgagcaac
     5221 gccgacgaac tcaagaatat cggcctcaag gcaacgacgc ccaggctgcg cgttctcgag
     5281 ctgttccagc atagcgagca gcgtcacatg accgccgagg atgtttacaa ggcgctgaca
     5341 ctcgagggct tcgatatcgg cctggcgacc gtctacaggg ttctcacgca gttcgagcag
     5401 gccggcctcc tcaatcgcca ccacttcgaa tccgaccgct cggtcttcga ggtcaacgaa
     5461 ggtacgcacc acgaccatct agtctgcctg cagtgtggcc gggttgagga attctttgat
     5521 ccggtgatcg aaaagcgcca ggcggaaatc gccgcacagc gcggctttac gatccgcgag
     5581 catgcgctct acctttacgt cgactgccag cgcaccgact gcgagcacca cccggcagcc
     5641 ggcgctgatg ccaaatcggg cggacccgcc gagtagtcgc ctggcaacac cataagctgc
     5701 tagcatccgc gccccccttg gcgcgagaag cgtcgctact gcaagatcat ggacgccttc
     5761 ctgacctcca cgctgctcgt cgccatcgcc gaaatcggcg acaagacgca attgctgtcg
     5821 ttcgttctcg ccgcgcgctt gcgtgcgccg ctggcgatca tctgcggcat cttcgtcgcc
     5881 accgtggcca atcacgcgct ggccgggtcg gtcggggcct gggtcgccac gctgctcgcg
     5941 cccgaggtgc ttcgctggct ggttggcctc ggcttcatcg ccttcggtct gtgggcgcta
     6001 aagcccgata cgctggaaga cgagcccagt gcctcgcgct ggggcccctt catcacggcg
     6061 accgtcgcgt ttttcatcgc cgagatgggc gacaagacgc aactggctac cgtggcgctc
     6121 gctgcgcgct tcgatgcgct ggccccggtt gtcgtcggta cgacgctcgg catgatgatc
     6181 gcgaacgtac cggccgtact tgtcggcagc aaacttgccg atcgccttcc gctggggatc
     6241 attcgctggg tggcggccgg cggtttcgtt gccactggcc tgtggacgct ctggccgcgc
     6301 tgaaccgtgg ccgctcaggc actggcctga aaggtcgctg gtgcgttctc gcccgtttcg
     6361 acagcccgat agagcgacag tcgcccatcg tcatcgctcg tctggctaac ttctgatcct
     6421 gcagtggcct cctctcgggc aagggtctgg atctgcgccc gggcttccgc ctccattgcc
     6481 gccgcctgcg ccgcaacccg gcgatcggtg tttgatggat caacgggcgc caaggctgcg
     6541 cggcggatct tctgcgcctt ggccagcgtt tcttccgggg tgcgtccggg actgccgtcg
     6601 atcgggactt caccgccagt cgcatagcgc tggccatccg gaccaaccgt gtactggtag
     6661 cgagcgccgc cactaaccac cccggcaccc gccgaaaggt gggcaaactc gtgcgcacga
     6721 acacgctggt caatacgccg cagttcggca atttggcgcg cctcggccgg cgtctgccct
     6781 gtggcttccg tctggcgggc atccgcacgc aagccgcccc gttcctgcgg ggccagcggg
     6841 ccaatacgtg caaatgttgc gctgccaatc tccatggcca cctcctgatg ccggtgggct
     6901 agcttactga agactagtca atcgcatcga caacggccag catttcccgg gcgtgttgcc
     6961 gcgacaccgg cgtgatttcg acgccgccga gcatccgtgc gatctcttct tcgcggcttg
     7021 ccgcactcag ccgctcgacc gcgctgacga aggcatcgcc gcgtcgtgcc ttactgacac
     7081 gccactgcca gtccgcgcac gcagccacct gtggcaggtg cgtgacgcag agcacctgcc
     7141 gattgcggcc cagagagcgc atcagccggc cgacaacctc ggcaacgcca ccgccgatgc
     7201 cgacatcaac ttcgtcaaac accagcgtcg gcaccttggc cgcgcggctg gcgacaacct
     7261 gcagcgccag gctgatccgg gacaactcac cgcccgaggc gacccgtgcg agcggcttca
     7321 tctcgctgcc ggcgaggccg ccaacgcgga actcgacgcg ttcaagtccg aagctgccgc
     7381 cctcctcaac gggcgtcagc gtcacctcga aactgccacc accgaacgcg agagtaccca
     7441 tctgcgcgct gacctctttg ccgagctgcg tggcgacggt cctccgtgcg gcgccaaggc
     7501 gctcggcaag cgtgcgaaac cttgcttcgg ctgctctgga cgcttcggcc agtttctcgg
     7561 gattcgccgc gtcgccgagc gcggcgagtt gccgctcccg ctcaaccagg gcgtcgccga
     7621 gatcgtttgc ctgaatgcgg aacttgcgcg cggtgccgag aatcgcctca agccgctggt
     7681 cgacctcggc cagccgcgac ggatcaagat caactcgctc gccgtagcgg cgcaagcccg
     7741 ccagcgcctc gccgacttcg gcctccgccg aacgaacaag ggcgccaagc tcggcgagcg
     7801 acgcatcgta ggcggccagt tcgtcgagcc gcgccgcgac cgccgacagc tgggacgagc
     7861 aggagtactc ggcctcgccg agggcctgca cacagtaggt cgagccttcg agcagcgcga
     7921 cggcgttggc cagtttcgcc tgctcggcgt taagcgactg ccactcatca gggctgaagg
     7981 cgagcgcgcg cagttcgtcg acttcccagg ccaggctttc acgttgccgc cgcaatgcct
     8041 caccgtcggt cagcgacgcc tgccaatgag cctgcgcatc gcgccagcgg cggtaggtgt
     8101 cggccacctc cgccgccaac tcgacgagat cgccgtaggc atcgagtagg tctcgctggg
     8161 cgtcggcacg aagcagcgac tggtgcgcgt gctgaccatg gatgtcgacc agcaggtcgg
     8221 tgatttcacg gagttgctgg agcgtcaccg gggttccatt tagccaggca cgcgagcgac
     8281 cgccggcatc gacgacccgg cgcagcagca gcacgccgtc ctcgtcgcca agttcctggt
     8341 cggcgagtac gcggcgaacc atgtcgttgc ccgcgacctc gaattcagcg ctgacttcgg
     8401 cgcgctcagc accggcgcgg atcaggccgg tatcggcgcg ttcgccgagg acgaaagcca
     8461 gcgcgtcgac cagaatcgac ttgcctgcgc cggtctcgcc ggtaagcacg ccaaagccgc
     8521 tctcgaacgg cagttcaagc cgatcgacca gcacgaaatc ccggatcgtc aattgcttca
     8581 gcatcacccg aaccctcgac tcagcccttg tttccgaccg ggcgctcgct ccagtggagt
     8641 ttctgccgaa gcatggcgaa atagctgtag gccggcggat gcaggaaacg gatagcgtag
     8701 tccgagcggc ggattcgtac cgagtctccg ggtcgcaggt cgcaggtaac ctggccgtcg
     8761 aagtgcacgc gcggatcggc accgtgtgtc acccgcagtt cgagctcgct ctgttcgccg
     8821 acgatcaccg gccgatttgt cagcgcatgc ggacacaacg gcacgagccc gatcgcctgc
     8881 aggcttgggt gcataaccgg gccgttagcc gatagcgcgt aggcggtcga ccctgtcggc
     8941 gtagtgacga tcaggccatc ggcacgcagc gtgtagatga actccccatc gatcgtcagg
     9001 tcgatttcga tcaggcgacc aatcgcaccc ttgtcgatca cgatgtcatt gagggcgagg
     9061 ttggcggtga tctcgcggcc gtcgcggatg acctcggcgt cgagcatcat ccgtacttcg
     9121 ggcacgaaac gcccgtcgag caggtcttcc atcgtcgtca gcatctcgct gcgcgcgatg
     9181 tcggtcatga acccgaggtg gccctggttc acgcccacca gcggcacacc gaaacgcgcc
     9241 agctgccgcg ccgcattgag catcgtgccg tcaccgccga ggacgatcgc cacgtcggcc
     9301 tgcgcgccga tcgcagcaaa agcggccggc acgaactcct gcaccgcctg cggtaaggcc
     9361 cgcttcgcgg tttcttcctc gacgtacacg gtgacgccac gttcgcgcag gaagcggccg
     9421 agagcgacca acgcctcgcc gatctcgggg ctgccaaact tgccgatgag ggcgacggta
     9481 cgggggattt gctctgaacg catggacgag gattaaacca caacccattt cgccgatggc
     9541 aagcgcgacg ggcgccacta caggccgcat tgggtagaat cgccacatgc tcgacgaacg
     9601 cgcccgcatc ctccttaaaa cactggtcga acgctacatc accgacggtc aaccggtcgg
     9661 ctcacgcgcg ctttccaagg tttctggcct cgacctgtcg gcggcaacgg tgcgcaatgt
     9721 catggccgac ctcgaggaaa tgggtttcat tgccagcccg catacctctg ccggtcgggt
     9781 accgacggcg cgcggctacc gtttcttcgt cgattcgctg ctcaccgttc aggcgctcga
     9841 acgcgaaaag ctcga
//
LOCUS       DATDAA010000453         6961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_277246,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000453 DATDAA010000000
VERSION     DATDAA010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277246"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VIS73_13670"
     CDS             complement(<1..863)
                     /locus_tag="VIS73_13670"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5764252.1"
                     /translation="MPHLVRIAALTAGLTLLAGCTVFGPTPHIADTSAIDSGRLPEPD
                     LSLDIPGLGPCTDSPDRRVQLNASHPVTVMVHGCFGSAGRFRALSDVLAFHGQQNVCF
                     SYDDRDSLEKSSAELARALERLASSMPRSAINVIGHSQGGLVSRRALISERDDALTTD
                     SPIRLVTVSAPFSGIRAARFCAYTWLRVASLGLQDLACRAVSGDKWYEITYASDLINQ
                     PGELLPVVNDHLKIVTDERDSCRRRSAAGACLEDDHVFELAEQYQSAVDADRRVANVE
                     IRAGHVEIVGNA"
     gene            complement(923..1780)
                     /locus_tag="VIS73_13675"
     CDS             complement(923..1780)
                     /locus_tag="VIS73_13675"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5764253.1"
                     /translation="MNEAANAFDATELDFEERVVAASHAVPIVVDFWAPWCGPCTTLK
                     PILERLASEYGGRFRLARVNSDEAPSLAARYQVRSIPSVKAFVSGRIVDEFVGVLPEA
                     AIRQFLDRLQPTPAELMRNEARELAARESFAEAIALLQQALADDPRHEGTRLDLAELH
                     VRQRDFAMAETVLDHVFVDEADRAQALRATLSLASASGDLASLRAAVARDETDAAARL
                     ALGRALAADGDYPGALEQLLASVRLDRALDDQLARRTVLQLFDLLASDPARDDLVREY
                     RRALASALN"
     gene            1903..2688
                     /locus_tag="VIS73_13680"
     CDS             1903..2688
                     /locus_tag="VIS73_13680"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5764254.1"
                     /translation="MIEAEPKDPAPFAGADIPKNAAATTWRWTLAALVPAVAALWLVV
                     YLNTGPESAAALPASLFTVLAVAVTGLAAAALVSMAQVVRSYGELEDRVRELASRDSL
                     SGAYNHVAFIDILDREFARSRRYGQPLALLIFDVDFFRRIHDGYGSLVGATALQAFAR
                     VLQAGLRNVDAVGRLGGEEFAVMLPNTPAQQALLLADRLREDAAAIVVRAQEGEDVRF
                     TTSVGVAEMTSDDVGVEDLIRRSDAALGKAKDLGRNRAHLAET"
     gene            2808..6749
                     /gene="purL"
                     /locus_tag="VIS73_13685"
                     /gene_synonym="purI"
     CDS             2808..6749
                     /gene="purL"
                     /locus_tag="VIS73_13685"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEY5764255.1"
                     /translation="MTLLYFRGKPALSAFRNERLLSRLQAVVPRVSALSAEYWHFVAL
                     TEPGANLAAPFARLLDAESATATDESGQVLVTPRLGTISPWSSKATDIARNCGLSGFE
                     RVERGIRFQLTMRDPGPLTDDERRGVLPLLHDRMTESVLERFDEVEKLFAHFAPRPLA
                     SIDLLKNGRAALVEANRKLGLALADDEIDYLGRVFGEAGRNPSDVELMMFAQANSEHC
                     RHKIFNASWIIDGQAEDRTLFGMIRETHRANSEGTIVAYSDNAAVIEGAAAERLYPDA
                     DGVYRRHAELTHFLAKVETHNHPTAISPFPGAATGAGGEIRDEGATGRGAKPKAGLCG
                     FSVSNLEIPGLAQPWEASYGRPERIASALDIMIEGPIGAAAFNNEFGRPNLCGYFRSF
                     EQRVDGVVRGYHKPIMIAGGIGNVQDGQALKVEQIPAGTLLIQLGGPGMLIGLGGGAA
                     SSVSSGVNAADLDFASVQRGNPEIQRRAQEVIDRCWQQGAANPILSVHDVGAGGLSNA
                     FPELAHGGGAGARFELREIPIEEPGMSPAEIWSNESQERYVLAIPPERLESFRAICLR
                     ERCPFAVVGEATDDGRLLVTDRQFGDSPVDMTMDALLGKPPRMTRNVTRLAVEREAFG
                     FGDIDFAEAVHRVLALPTVADKSFLITIGDRTVGGLTARDQMVGRWQVPVADVAVTLL
                     DYSGYAGEAMAMGERTPVALIDAPASGRMAVGEAITNLLASPVDTLRRVKLSANWMAA
                     AGFPGEDAGLFDTVEAVSKLCQSLGISIPVGKDSLSMRTAWDDGDAKKEVVAPLSLIV
                     SAFAPVDDVRLTLTPELRLDAGETELLLIDFSAGKNRIGASALAQVFGGLGSQAPDLD
                     DATLLTGFCEAMRRLRAEGLVLAYHDRSDGGLLAAVCEMAFAAHCGVTLNVDVLCYDP
                     LAHDVDGNERKPQLMDGRSSELLLRALFAEELGAVLQIRHEDRDCVCSILREAGLGRC
                     FNLIGYPNDRDEIRVVRNARAVFAEARASLQGRWSSTSLEIQSIRDNPACIAEARAAL
                     ESTAARGLWARPSFDIAVDVAAPFIARGARPKVAILREQGVNSQLEMAAAFERAGFDP
                     VDVHMSDLVSGRRTLDASLKGVIACGGFSYGDVLGAGLGWARSILFNERCRDDFAAFF
                     ARPDTFSLGVCNGCQMMSALKEIIPGAGAWSRFERNRSEQFEARLVMAGVPDSPSMFF
                     AGMAGSEMPIVVSHGEGRADFQTTGSRQDARVALRYLDDSGKAASRYPENPNGSPDGI
                     TGLTSDDGRATILMPHPERVFRAVQMSWRPREWAEDSPWMRMFRNARAWVG"
BASE COUNT         1175 a         2240 c         2351 g         1195 t
ORIGIN      
        1 gcgttgccga cgatctccac atggccggcg cggatctcga cattggcaac gcgtcgatcg
       61 gcgtcgaccg ctgattgata ctgctcggca agctcgaaca cgtggtcgtc ttctaggcag
      121 gcgcccgccg cggagcggcg ccggcagctg tcgcgttcgt cggtgacgat cttcagatga
      181 tcgttgacga ccggcagtag ttcgccgggc tgattgatca ggtccgaggc ataagtgatc
      241 tcgtaccact tgtcgccgct gacagcgcgg caggcgagat cctgaaggcc caacgacgcg
      301 acgcgcagcc acgtgtacgc acagaagcgg gctgcgcgga tgcccgaaaa gggcgccgag
      361 accgtgacca gacgaatggg cgagtcagtc gtaagcgcgt cgtcgcgctc gctgatcaag
      421 gcgcgccgcg agaccaggcc accctgactg tggccgatca cgttgatcgc cgaccggggc
      481 atgctagacg cgagccgctc aagcgcccga gccagctctg ccgaggattt ctcaaggctg
      541 tcgcgatcat cgtaggaaaa gcagacgttt tgttgtccgt ggaaggcaag cacgtcggag
      601 agcgcacgga aacggccggc cgagccgaag cagccgtgga ccatgaccgt gaccgggtgg
      661 ctggcgttga gctgcacccg gcgatcgggc gagtcggtgc acggaccaag tccggggatg
      721 tcgaggctga ggtcgggttc cggcagccgg ccggagtcga tcgcactggt gtctgcgatg
      781 tggggcgtcg gcccaaagac ggtgcagccg gcgagcagcg ttaacccggc agtcaatgct
      841 gcgattcgca cgaggtgagg cattgccgcg atgctcctca agtgggttgg cgggctgtca
      901 attcagggcg tccgagtgtg aactaattca gcgcgcttgc cagcgcccgt cgatactcgc
      961 gcacgagatc gtcgcgtgcg gggtccgacg ccagcagatc gaaaagctgc agcaccgtcc
     1021 gccgcgccag ttggtcgtca agagcacggt cgaggcgaac cgaggcgagc agctgttcca
     1081 gggcgccggg gtagtcgccg tctgcggcca gtgcgcgacc cagcgcaaga cgcgcggcag
     1141 catccgtttc gtctcgggca acggcagccc gcagcgacgc cagatcgccg ctggcgctcg
     1201 ccagcgacag cgtggcacgc agtgcctgcg ctcgatcggc ctcgtcgacg aagacatggt
     1261 cgagcacggt ttcggccatc gcaaaatcac gctgccggac atgcaactct gcaaggtcaa
     1321 ggcgcgttcc ctcgtgccgc ggatcgtctg ccagcgcctg ttgcagcagc gcgatcgcct
     1381 cggcaaacga ctcacgcgcc gcgagttccc ttgcttcgtt gcgcatcagt tcggccgggg
     1441 tcggctgcag gcggtcgagg aactggcgga tcgcggcttc gggaaggacg ccgacgaatt
     1501 cgtcgacgat gcggccgctg acgaaggcct tgacgcttgg aatcgagcga acctgataac
     1561 gtgcagcgag actgggcgcc tcgtcggaat tgacccgagc gagccggaag cggccgccgt
     1621 actcggaagc caagcgttca aggattggct tgagcgtcgt gcaggggcca caccagggag
     1681 cccagaaatc aacgacgatc gggaccgcgt gcgatgcggc gacaacgcgt tcctcgaaat
     1741 ccaattcggt ggcgtcgaag gcgttggcgg cttcattcat gggattgcag acttgcgtag
     1801 ggaaatcatt gatgcgcaca atgctaacag actgaaatgg cgccatcggc tgcgcgccgc
     1861 cgccgaccaa ccgcagaacc aacagggcag ggaacatttg ggatgatcga agccgagccg
     1921 aaggacccgg cgccgtttgc cggcgccgac attccgaaga acgcggcggc caccacgtgg
     1981 cggtggacgc ttgccgcact ggtaccggcg gtcgcagccc tctggctggt cgtctatctc
     2041 aataccggac ccgaatcggc ggcggccttg cccgcatcgc tgtttacggt gctcgccgtc
     2101 gccgtaaccg gcctggcggc agcggccctg gtgtcgatgg cacaggtcgt tcgttcgtac
     2161 ggcgagcttg aggatcgcgt tcgcgaactg gccagccgcg attcgctgag cggtgcgtac
     2221 aaccacgtcg cgttcatcga catcctcgac cgcgagttcg cccgcagtcg ccgttacggg
     2281 caaccgctgg cgctgttgat cttcgacgtc gatttctttc gcagaatcca tgacggatac
     2341 ggttcgctgg tcggcgccac ggctctgcag gcattcgcca gggtcctgca ggccgggctg
     2401 cgcaatgtcg acgcagtcgg ccggctgggc ggcgaggaat tcgccgtcat gcttcccaac
     2461 acaccggcgc agcaggccct cctgctggcc gaccgcctgc gcgaggatgc ggcagcgatc
     2521 gtggttcgcg cgcaggaggg cgaggatgtc cgtttcacca ccagcgtcgg cgttgccgag
     2581 atgacctccg acgacgtcgg cgtcgaagac ctgattcgcc gcagcgatgc ggccctgggg
     2641 aaggccaagg acctcgggcg caaccgcgcc catctcgccg agacctgagc ccggcccggc
     2701 tgcgccgggt gcccggcatg ttggcgcctc cgctataatc cgcgcgccgc tcccgcgcat
     2761 cgattcggtg cgtcggcggt tccgcccgac gcacccgtag agacccgatg accctcctgt
     2821 acttccgcgg gaaacccgcg ctttcggcgt tccgcaacga acgcttgctt tccagactcc
     2881 aggcagtggt accgcgcgtc tcggccttgt ccgccgagta ttggcatttc gttgcgctga
     2941 ccgaacccgg cgctaacctc gctgcgccct ttgcgcgcct gctcgacgcc gaatcggcaa
     3001 ccgccaccga cgaaagcgga caggttttgg tgacgccgcg actgggcact atttcgccgt
     3061 ggtcgtcgaa ggccaccgat attgcacgca actgcggcct ctccggtttc gagcgtgtcg
     3121 agcgcggtat ccgcttccag ctgacgatgc gtgatcccgg tccgctgacc gacgacgaac
     3181 gacgtggcgt gctgccgctg ctgcacgatc ggatgactga gtcggtcctt gagcgcttcg
     3241 atgaggtcga gaaactgttc gcgcatttcg cgccgcgccc gctggcgagc atcgatctgc
     3301 tcaagaacgg ccgtgccgcg ctggtcgagg ccaaccggaa actaggtctc gcgctggctg
     3361 acgacgagat cgactatctc ggccgcgtct tcggcgaggc cggccgcaat ccgagcgatg
     3421 tcgaactgat gatgttcgcg caggcgaact cggaacactg ccggcacaag atcttcaacg
     3481 cctcgtggat tatcgatggg caggcggaag atcggacgct gttcgggatg atccgcgaaa
     3541 cgcatcgcgc gaattccgag ggaacgatcg ttgcctactc ggacaacgcc gcggtaatcg
     3601 aaggtgcggc ggccgagcgc ctctatcccg atgccgacgg agtctaccga cggcacgcgg
     3661 aactgacgca cttcctcgcc aaggtcgaaa cgcacaatca cccgacggcg atctcgccgt
     3721 ttccgggagc ggcgaccggc gccggcggcg agatccgcga cgaaggggcg actggtcgcg
     3781 gcgccaagcc gaaagctggg ctctgtggct tctcggtatc gaacctggag atccccggcc
     3841 tcgcgcagcc ttgggaagcc agctacggac gtcccgaaag gatcgcctcg gcgctcgaca
     3901 tcatgatcga aggcccgatc ggcgccgctg ccttcaacaa cgagttcggc cgtccgaatc
     3961 tttgcggtta tttccggagc ttcgaacagc gagtcgatgg cgtcgttcgc ggttaccaca
     4021 agccgatcat gatcgccggc ggcatcggca acgtgcagga cgggcaggcg ctcaaggtcg
     4081 agcagatacc ggccggcacc ctgctgatcc aactcggcgg ccccggcatg ctgattgggc
     4141 ttggtggtgg cgcagcctca tcggtatcgt caggcgtcaa cgccgccgat ctcgacttcg
     4201 catcggtcca gaggggcaac ccggaaatcc agcgccgcgc gcaggaggtc atcgaccgct
     4261 gctggcaaca gggtgccgcg aacccaatcc tgtcggtcca cgatgtcggt gccggcgggc
     4321 tgtcgaacgc cttccccgag ctggcccacg gtggcggcgc cggtgcgcgc ttcgaactgc
     4381 gcgagattcc gattgaggag ccggggatga gcccggccga gatctggagc aacgagtcgc
     4441 aggagcgcta cgtactggcg atcccgcccg agcgactgga gtcgttccgg gcgatctgcc
     4501 tgcgcgagcg ctgtccgttc gccgtggtcg gcgaagcgac cgacgacggg cgtttattgg
     4561 tgaccgaccg ccagttcggc gattcgccgg tcgacatgac gatggatgcg ctgctcggca
     4621 aacccccgcg aatgacgcgg aacgtgacgc ggcttgcggt cgagcgcgag gcattcggtt
     4681 tcggggacat cgatttcgcc gaggcggtgc atcgcgtgct ggcgctgccg acggtcgccg
     4741 acaagagctt cctgatcacc atcggcgatc gcacggttgg tggcctgacc gcgcgcgacc
     4801 agatggtcgg ccgctggcag gttccggtgg ccgatgtcgc ggtcacgctg ctcgattaca
     4861 gcggctatgc cggcgaagca atggcgatgg gcgaacggac accggtcgcc ttgatcgacg
     4921 cgccggcgtc ggggcgcatg gcggtcggcg aggcgatcac caatctgctc gccagcccgg
     4981 tcgatacgct gcgccgggtc aaactgtcgg ccaactggat ggctgcggcc ggcttcccag
     5041 gtgaggacgc gggtctgttc gatacggtcg aggcggtttc gaagctgtgc caatcactgg
     5101 gcatttcgat tccggtcggc aaagattccc tgtcgatgcg cacggcctgg gacgatggcg
     5161 atgcaaagaa ggaggtcgtc gcgcccctat cgctgatcgt cagcgccttc gcgccggtcg
     5221 atgacgtgcg gctgacactg acgcccgaac tgcgactcga tgccggcgaa accgaactcc
     5281 tgctgatcga cttttcggcc ggcaagaacc gcatcggcgc ttcggcgctg gcccaggtct
     5341 tcggtggcct tggttcgcag gcccccgacc tcgacgacgc cacactgctc accggtttct
     5401 gcgaggcgat gcggcgactt cgtgccgagg gcctggtgct tgcctatcac gaccgctccg
     5461 acggcggcct gctggcggcg gtctgcgaga tggctttcgc tgcccactgc ggtgtcacgc
     5521 ttaacgtcga cgtgctctgc tacgacccgc ttgcccacga tgttgacggc aacgaacgca
     5581 aaccgcagct gatggacggg cgatcgagtg aattgctgct gcgtgcactg ttcgcagaag
     5641 aactgggcgc ggtcttgcag attcgccacg aggaccgcga ctgcgtctgc tcgatcctgc
     5701 gcgaagctgg cctcggccgc tgctttaacc tgatcggcta tccgaacgat cgcgacgaga
     5761 tacgcgtcgt gcgcaacgct cgcgccgttt tcgccgaggc tcgggcctcg ctgcagggtc
     5821 gctggtcgag taccagcctg gaaatccagt cgattcgaga caatccggcg tgcattgccg
     5881 aagcacgcgc agcgctggaa agcactgccg cgcgcggcct gtgggcgcgt ccgtcgttcg
     5941 acatcgcggt tgatgtggca gcgccgttta tcgcgcgtgg tgcccggccg aaggtcgcaa
     6001 tcctgcgcga gcagggcgtc aacagccagc tcgaaatggc cgccgccttc gaacgcgccg
     6061 gcttcgatcc ggtggatgta cacatgagcg acttggtctc cgggcgtcga acgctggacg
     6121 cgtcgttgaa gggcgtcatc gcctgcggcg gcttctccta cggcgacgtg ctcggtgcag
     6181 gtctgggctg ggcgcgttcg atcctcttca acgagcgctg ccgggacgac ttcgcagcct
     6241 tctttgcccg tcccgacacg ttctcgctcg gagtctgcaa cggttgccag atgatgagcg
     6301 cgctcaagga gatcattccg ggagctggag cctggtcgcg cttcgagcgg aaccgcagcg
     6361 agcaattcga ggcacggctg gtcatggccg gcgtccccga ttcgccatcg atgttctttg
     6421 ccggcatggc cggcagcgag atgcccatcg ttgtttcgca cggggaaggg cgcgctgatt
     6481 tccagaccac cggctcacgg caagatgcgc gggtcgcgct gcgctacctc gacgactccg
     6541 gcaaggcagc ctcgcggtat ccggagaacc caaacggctc tcccgatggc atcaccgggc
     6601 tgaccagcga cgacggccgg gcgacgatcc tgatgccgca tccggaacgc gtgttccgcg
     6661 ccgtgcagat gagctggcgg ccgcgtgagt gggccgagga cagcccatgg atgcgcatgt
     6721 tccgcaacgc gcgcgcctgg gttggctgaa tggagcgcgc ctagcgggat ggtgtatggt
     6781 gccggcatgg agatcgacaa gacccaagcc gtcgccatcg ccgccttcag ccgcttcgac
     6841 gaagacgtgc acgacccctt gctgcgcgcg gtgagcgcgg tgtactgcct cgtcgcctgc
     6901 gccgacggcg acctcgacga caccgagctg gccgagtacg tgcgcgtggc ccaggccgac
     6961 t
//
LOCUS       DATDAA010000454        22392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_103097,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000454 DATDAA010000000
VERSION     DATDAA010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 22392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22392
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103097"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1995)
                     /gene="glmS"
                     /locus_tag="VIS73_13690"
     CDS             complement(160..1995)
                     /gene="glmS"
                     /locus_tag="VIS73_13690"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY5764256.1"
                     /translation="MCGIVAAAARRNIVPVLIEGLRRLEYRGYDSAGIAVLAAGDGLQ
                     RLRSVGRVAELAQQAERNNTESATGIAHTRWATHGVPSERNAHPHISGKLAVVHNGII
                     ENYATLKQELTKQGYVFTSDTDTETIAHLIEHLLKQEADLHAAVRAATRRLSGAYAIA
                     VVHTDCPDTVVVAREGAPLLLGLAADGVYAASDASALLQVTRRIVYLENGDIAEIKPD
                     QWRVTLADGTAVTREETESHLSADAIELGSYRHYMQKEIFEQPEALANTLEMIGAARS
                     IQPGLFGGDAEIALREVDSILIIACGTSSHAGMTARYWLEAIAGLPCTVEIASEYRYR
                     RSVPNPRQLVIAISQSGETADTLAALRHAKALSHRHTLALCNVPESAIVREAGLRFIT
                     RAGAEIGVASTKAFTTQLAALFLLTLVLAKLRGQLNEAAETRYLDALRHLPVAVQKIL
                     EQEEAIRGWAERFAMKQHALFLGRGMHFPIALEGALKLKEISYIHAEAYAAGELKHGP
                     LALIDKDMPVVAVAPNDALIDKLKANLQEVRARGGELYIFADADCDMDADSEGVHLIR
                     LPEHYGELSPLLHVVPLQLLAYHAALVKGTDVDKPRNLAKSVTVE"
     gene            complement(2005..3363)
                     /gene="glmU"
                     /locus_tag="VIS73_13695"
     CDS             complement(2005..3363)
                     /gene="glmU"
                     /locus_tag="VIS73_13695"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEY5764257.1"
                     /translation="MLNVVILAAGQGKRMHSNLPKVLHPLAGKALAAHVIDAARSLRP
                     DRLCVVYGHGGETVREALSADDLSWAKQEPQLGTGHAVMQAMPLLTDSGTTLVLYGDV
                     PLIRAETLTRLLQVAGEGIAVLTVELDQPRGYGRIVRDAAGKVTGIVEEKDATSDQRA
                     IREINTGIMAIPSARLAGWLGRLSADNAQGEYYLTDVIGLAVADGVPVTTVHPAHDWE
                     VAGVNSKVQLAELERCQQLDIAQQLLEAGVRLADPARLDVRGTLRCGRDVAIDVGCVF
                     EGEVSIEEGAEIGPYCVLRNVRVAAGARVVAFTYAESAVIGPDATVGPFARLRPGAEL
                     GPESHVGNFVELKNSYLGAGSKANHLAYVGDTTVGSRVNIGAGTITCNYDGANKFRTI
                     IEDDVFIGSDTQLVAPVTVGRGATLGAGTTLTQDAPPESLTISRVKQISITGWQRPQK
                     KK"
     gene            complement(3468..4934)
                     /locus_tag="VIS73_13700"
     CDS             complement(3468..4934)
                     /locus_tag="VIS73_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEY5764258.1"
                     /translation="MGKPTGFIEYDRLTEAAEPADKRVKHYREFVLRLSDEEASLQGA
                     RCMDCGIPFCNDGCPVHNIIPDWNDLVYRGDWKNALDVLHSTNNFPDFTGRVCPAPCE
                     AACTLNINAAPVGIKSIEHAIIDKGFEMGWVVPQPPKSKTGKQVAVVGSGPAGLAAAQ
                     QLARAGHEVTVFEKSDRIGGLLRYGIPDFKLEKSHIDRRVAQMEAEGVVFRTGIFIGK
                     GVGEPGVLNLAKSSIDPEKLVADFDAVVLAGGSEMPRDLAIPGRELKGVHFAMEFLPQ
                     QNRVVAGDKIKDQILATGKHVVVIGGGDTGSDCVGTSNRQGALSVTQFELMPQPPVEE
                     NKPFVWPYWPIKLRTSSSHEEGCERDWAVSTKSFIGEGGKLKALKVVRVEWKDGKMQE
                     VPGSEFEIKADLAFLAMGFVNPMQSVLDAFGVDRDHRGNARAEVAGEKAYSTAADKVF
                     AAGDMRRGQSLIVWAIREGRQCARAVDEFLMGSSTLPR"
     gene            complement(4975..9609)
                     /gene="gltB"
                     /locus_tag="VIS73_13705"
     CDS             complement(4975..9609)
                     /gene="gltB"
                     /locus_tag="VIS73_13705"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEY5764259.1"
                     /translation="MSPMLPETQGLYDPAYEHDACGVGFVAQMRGERSHSIVEQGLLI
                     LKNLDHRGAVGADPLQGDGAGILIQIPDALYREEMAKQGITLPPAGEYGVGMVFLPRE
                     SASRLACQEEIERAIRAEGQVLLGWRDVPINREMPMSPAVRKTEPVIRQVFVGRGPDV
                     LVTDALERKLYVLRRRGANAIKSLKLKHGQEFYMASFSARTVVYKGLLLATQVGEYYA
                     DLADPRCISALALVHQRFSTNTFPTWDLAHPFRMIAHNGEINTLRGNVNWFKAREQAC
                     ASPLLGDDLKKIWPLHYPGQSDSASFDNALELLVMGGYSMAHAMMLMIPEAWEKHTLM
                     DANRRAFYEYHAAMMEPWDGPAAVAFSDGRQIGATLDRNGLRPARYCVTDDGLVIMAS
                     ESGVLPVPESKIVKKWRLQPGKMFLIDMDQGRIIDDKELKDSLANAKPYREWIDKIRI
                     KLDEIPVADESREQPATKLLDRQQAFGFTQEDIKFIIEPMIANGEEPTGSMGNDAALP
                     VLSNRAKTFYNYFKQLFAQVTNPPIDPIREDLVMSVVSFIGPKPNVLGVNDINPPIRL
                     EVAQPVLSSGDIEKIRHIQKYTGGKFRSHELNICYPAAWGKEGIEARLASLAAEAEDA
                     VRSGANILIVSDRRMDADSVAIPALLATSAVHQHLVKNGLRTSTGLVVETGSAREVHH
                     FALLGGYGAEAVHPYLVFETLAELAGGDATKQSTYAKNYTKAVGKGLMKVMSKMGIST
                     YMSYTGAQIFEAVGLKSDFVAKYFAGTASKVEGIGVFEVAEEAIRLHSDAFSDDPVLA
                     TMLDAGGDYAYRIRGEEHMWTPHSIAKLQHATRAGQYHTYREYAKLVNEQGKRHMTLR
                     GLFELRPAAAPVPIDEVEPAKEIVKRFATGAISLGAISTESHTGLAIAMNRIGGKSNT
                     GEGGEDARRFPAVKGGTKLSEVIGEGRVQADYTLQDGDSLRSAIKQVASGRFGVTTEY
                     LVNADQIQIKMAQGAKPGEGGQLPGHKVSEYIGFLRHSVPGVGLISPPPHHDIYSIED
                     LAQLIHDLKNVNPKASVSVKLVSEVGVGTVAAGVSKAKADHVVIAGHDGGTGASPLSS
                     IKHAGTPWELGLAETQQTLVMNRLRDRIRVQVDGQMKTGRDVVIGALLGADEFGFATA
                     PLVVQGCIMMRKCHLNTCPVGVATQDPELRKRFTGQPEHVVNFFFFVAEEVRELMAEL
                     GIRRFEDLIGRSDLLDVKAGIDHWKANGLDFTRIFYMPPAASPAERRQEVLQNHGLDR
                     ALDHQLIKLAEPALTKGEKVSIDTTIKNINRTVGAMLSGRVAERYGSAGLPDDTIHVR
                     LRGTAGQSFAAFLACGVTFDLIGEGNDYVGKGLSGGRIIVRPHPDFSGAATENIIIGN
                     TVMYGATEGEAFFSGVGGERFCVRNSGATAVVEGVGDHGCEYMTGGTVVVLGETGRNF
                     AAGMSGGIAYVYDVDGQFAQRCNTAMVDLHPVRPAGRQPDSEPRHRGQEDEALLKSLI
                     ERHATYTGSARAKQLLAQWDNVRERFVKVFPKEYRRALTEMQKKDRKVAA"
     gene            complement(9834..10949)
                     /locus_tag="VIS73_13710"
     CDS             complement(9834..10949)
                     /locus_tag="VIS73_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEY5764260.1"
                     /translation="MADPAPYAISDADSRGRRYSEAAPNLRSQFQRDRDRIVHSTAFR
                     RLEYKTQVFVNHEGDLFRTRLTHSIEVAQIARSIARALRLNEDAVEAIALAHDLGHTP
                     FGHAGQEALDDCMRRHGGFEHNLQSLRIVDHLENRYAEFAGLNLCFETREGIVKHCSI
                     RNARTIGDVGERFIAKRRPSLEAQLCNLADEIAYNNHDVDDGLRSGLITIAQLEAVPL
                     FSRHLATVRNAYPQLAGRRVVHETVRRMINELVTDLIVTTTAKLEAVAPVTLEDVRDA
                     EPLVAFSDALWAEHRKLKQFLHQNLYRHYRVLRMTNKAQRVVRDLFGAFIADPRLLPP
                     KYQQDDNQPRSVADYVSGMTDRYALAEHKRLFSVDGT"
     gene            complement(10942..12042)
                     /gene="aroB"
                     /locus_tag="VIS73_13715"
     CDS             complement(10942..12042)
                     /gene="aroB"
                     /locus_tag="VIS73_13715"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY5764261.1"
                     /translation="MLKHELEIDLGERSYRILIGAGLLDDPRPIAGAISGKCAVIVTN
                     VTVGPLYADRLAGALNAAGIKTLRIDLPDGEQYKNWETLQGIFRTLLEHACERSTTVI
                     ALGGGVVGDITGFAAACYQRGVPFIQIPTTLLAQVDSSVGGKTAINHPLGKNMIGAFY
                     QPRLVVADTTVLNTLPPRELSAGLAEVIKYGLIRDAEFFDWIESNIGGLLRRDPETLA
                     YAIERSCSHKAAVVSADEREAGSRALLNLGHTFGHAIETGMGYGLWLHGEAVAAGTMI
                     AAEVSQRNGWLSKGDVDRIDALFRAAKLPTRGPAMSPDRYFELMQHDKKVKDGRIQYV
                     LLRGIGDAVLTANLEPHRVRDAIVARTPPADG"
     gene            complement(12036..12539)
                     /locus_tag="VIS73_13720"
     CDS             complement(12036..12539)
                     /locus_tag="VIS73_13720"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY5764262.1"
                     /translation="MVLVGLMGAGKTTIGKALARKTGWRFVDSDHEIENRTGVTIPVI
                     FEIEGESGFRQRETKVIAELLREPNIILATGGGAVLSPENRQTIRDCGTVCYLKAAPK
                     TLYQRTRHDRSRPLLQVADPMAVLASLLDIRGPLYEEIADIVIDVERNTSSQIVERLS
                     KEDLFTC"
     gene            complement(12593..14608)
                     /gene="pilQ"
                     /locus_tag="VIS73_13725"
     CDS             complement(12593..14608)
                     /gene="pilQ"
                     /locus_tag="VIS73_13725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEY5764263.1"
                     /translation="MNKRTIFALGAWAALAIGGIAHAEERNTLENVNVSAAGDQVVVR
                     LSFANPLTSQPPGFSLTKPARVALDFTETDAAIQTGAQSVTQAFLRSYNVAQAGNRMR
                     VVFNLDRPAAYQTQIDGKDLIVLMNAAPAIELASPTPSAEPFAIAQPAIQALTQIRDI
                     AFRRGKDGEARVLVDLQTANTAGIDIRKQGGSLIVEFSKTDLPASLARKLDVTDFSTP
                     VSSIDTFRRGDRVRMVVAGKGNWDHLAYQADNQFVVELRPVSQETAQLGSAGRYTGER
                     LSLRFRDYPVKEVLQAFSDFASFNIVISEAVGGTISLNLQDVPWDQALEIIMQQKGLD
                     KRVDGNVVMIAPREEIIARNKVKTEAIELEMPQDAVFQVNYLTADVAKKKLEEYLKFD
                     PKASKGNKLMSESGSNKLFIKAPESTLEDIRRVLKEIDLPPRQVLIEARIVEASDSFS
                     RDLGTRLGINDNHASRLAGQNNLVLQQLGASVALPVAGGNLNFLLTNATQTRTLSLEL
                     TALEADGNGKIISSPRVLAMNGESAKIEDGQEIPYLQASSSGATSVSFKKATLSLDVT
                     PTINADGRIGMEIKVNKDSPGAVVPGGVAINTKQVTTKVVVDNGGTVVIGGIYSESER
                     FDESGIPGLRDIPGLGWLFKTRTTSKQRSELLVFITPRIVNEQVTLQ"
     gene            complement(14605..15129)
                     /locus_tag="VIS73_13730"
     CDS             complement(14605..15129)
                     /locus_tag="VIS73_13730"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEY5764264.1"
                     /translation="MALAVALFAVGGCGDNEHEDLRQWMREASKDLRGNIKPLPQVRP
                     YEPLLYDADALRSPFDQGRIATGGGGGRSGGPAPDLNRPREPLEEFPLETMKFVGFFQ
                     DKKRLVAQVLVNNKTYDLRVGNYIGQNFGRVTQIVTEQNGEMIVLKEVIQEPDGAWVE
                     RDSELHLSGAGGKQ"
     gene            complement(15177..15845)
                     /locus_tag="VIS73_13735"
     CDS             complement(15177..15845)
                     /locus_tag="VIS73_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEY5764265.1"
                     /translation="MKAPAISTEKLKSLADDFKNLNPRDIGAWPFAPRMAVLGVVFVA
                     VIAAGWWFVWNDQLVTLENARAQEITLKQQFIDKKKQAVNLDLYIQQLNEVETSFGAL
                     LKQLPNKTEVDTLLVEINQAGLGRGLQFELFKPSAEIKKEFYAELPVALKLTGGYHDL
                     GAFAADIGRLSRIVTLNNMTIVSGKGETLSMDAIAKTFRYLDEEEVAAARRAATQAQK
                     GRRR"
     gene            complement(15842..16435)
                     /locus_tag="VIS73_13740"
     CDS             complement(15842..16435)
                     /locus_tag="VIS73_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEY5764266.1"
                     /translation="MIRINLLPHREEKRRFKRQQFFALGGLMAVLAAAIVGLGWSVIS
                     GYIGAQNEKNQFLQREIASLDKQIAQIKDLQTNIRLQVARKEIIESLQRDRAEPVAML
                     NELVKQIPDGVFIKSFKQTGANISLEGSSQSNARVSTFMRNLESSPVFQDPKLIETKA
                     ASGKERRVQDFRMTTTVTRTASEADADARQTPQRGRK"
     gene            complement(16432..17511)
                     /locus_tag="VIS73_13745"
     CDS             complement(16432..17511)
                     /locus_tag="VIS73_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY5764267.1"
                     /translation="MQIDLSIFSAKAKPLIGLDLSSSAVKMVELSFDSRNGYRVERYV
                     VEVLPKDVIVDGNVANLEVAAETVKRAWKRLGSSTRSVAMALPAASVITKKVIVPAGQ
                     REEELEVQVESEANQYIPFALEEVNLDFQVIGPAASSPDEVEVLIAASRKEKVEDRVA
                     VAEAAGLTASVMDVESHALLAAFELVQTQLPEAGDNQVVALVDIGGSVMNLTVLRNLQ
                     PVYTREQAFGGTQLTQNIARQYGMSFEEAEAAKRSGNLPENYESDLLRPFIDDLALEV
                     SRALQFFFTSTQFNQVDHIVLAGGTGALAGADEAVAARVQVNTVVANPFASMVLSDRV
                     RARNLLTDSASLMVACGLALRRFDE"
     gene            17668..19989
                     /locus_tag="VIS73_13750"
     CDS             17668..19989
                     /locus_tag="VIS73_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY5764268.1"
                     /translation="MKEQTRRWLLVSLYALGTAFLSFTALVGIAVLKAQSQLPTLEAV
                     TDYRPRIPLRVYTADGDLIGEFGEERRSFVTIDKIPNVLRQAIIAAEDERFYAHRGID
                     VLGVMRAAYANLIRGSRRQGASTITMQVARNFFLSTEKTLNRKLFEALLAIRIENNLS
                     KDQILELYVNQIYLGQRAYGFGAASQVYFGKTVSDLNLGEAAVLAGLPKAPSAYNPIA
                     NLKRAKQRQEYVLRRMHELGAIDDSQLADAIAAPIVTRKETTLVSYETPADHAAEMAR
                     QIAIERFGEDVYTRGYKVITTLRTRDQRAARAALENGVRAYDRRHGYRGPEAFLPFDA
                     KQRPTDEELEEALGEFSDSADLSLAVITEIDKRKVTVYRRGGETLTLSDGGLKFAAEA
                     LDRRAPARMRFRQGAVVRLARMTDGDWEISQVPDVEAALVAADPSTGAIRALVGGYDF
                     GRNQFNHVTQAMRQPGSSFKPFIYSAALERGYTPASVLDDTPVLLPPEETGGQPWEPK
                     NYDDLYDDPMRLRIALAKSKNMVAIRLLQAITPAYARDFAARFGIEPANNPAYLTMAL
                     GSGTATPWQMVGAYSVFANGGYLVMPYLVDEIRDENNQVIARFEPRRAGTEDLRAIDP
                     RNAWTMDSMLRDVVAYGTAVGAGRALKRLDLAGKTGTTNDNVDAWFTGYHRSVVAVSW
                     IGFDQPKQLGSGETGGRAALPIWIDYMREALADTPIETDPPPPPPGMSLIQAIDLSVP
                     EGVIDEWVYDENVPPEPPVPSLWSPFDPLLPGN"
     gene            complement(20009..20326)
                     /gene="cyaY"
                     /locus_tag="VIS73_13755"
     CDS             complement(20009..20326)
                     /gene="cyaY"
                     /locus_tag="VIS73_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEY5764269.1"
                     /translation="MDDKEFDQRANETLEWIDGALESCGIDLDLNQLAGGVLEVEFPD
                     GSQLIINRHAAAREIWVAARSGGFHFRFDGTDWVDTRSGERLVAALSRLVSLQAQQPV
                     ALE"
     gene            20514..21758
                     /gene="lysA"
                     /locus_tag="VIS73_13760"
     CDS             20514..21758
                     /gene="lysA"
                     /locus_tag="VIS73_13760"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY5764270.1"
                     /translation="MSFTLIDGELHAEQLPLSEIAARFGTPCYVYSRQALTSALQEFQ
                     SALEGTDSLVCYAVKANSNLAILNLFARQGAGFDIVSLGELTRVIAAGADPGKVIFSG
                     VGKRSDEIEAAIMAGIGCFNVESEPELSRIDEIATRLGKKAPISLRVNPDVDPKTHPY
                     ISTGLRENKFGVDWQSARRIYQHAASLRGLEVVGIDCHIGSQILDVAPFLEALERILD
                     LIDQLAADGIVLRHIDLGGGLGIRYRDETAPAIADYLGAVRDRLAGRKLKLLLEPGRR
                     LVGNAGVLLTRVEYIKSGPEHNFAIVDAAMNDLLRPALYDAWHDIVPVRTRPGESRRY
                     DVVGPVCESGDFLAKDRSLSIAAGDCLAAMSAGAYGMTMSSNYNTRPRAAEVMVDGSV
                     AHLIRRRESIAELYAQETVLPA"
     gene            complement(21787..>22392)
                     /locus_tag="VIS73_13765"
     CDS             complement(21787..>22392)
                     /locus_tag="VIS73_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY5764271.1"
                     /translation="NTFVPPMMFNNSGNMGLPLMMLAFGEAMLPAAVMLFLVENVLHY
                     SLGTWMLDHRARLTTLWRIPVILAAIAGLAVSLSGFQPWPPFLTAVRMLGDVSIPLLL
                     FSLGVRLTDSAFQDLRIGLIGAVFCPLSGVLIAYAAALLLGIEGIQRDMLIVFGALPP
                     AVLNYVFAERYHQEPQRVASIVMVGNLGALIAVPLALALVL"
BASE COUNT         4178 a         6978 c         6896 g         4340 t
ORIGIN      
        1 gacacgccta ggaccgcggc tgtcgtacgc atttgcctgt tcattcgcca agttccgcca
       61 gagacaacag agcgcacgct acgcaagctg cacgcatgcg gcaatatgcc ctttgtagtc
      121 ggtcacctga cttgaagtaa aacgaccgcg ccgaccgcgc tactcgacag tgactgattt
      181 cgcgaggttg cgcggcttgt cgacgtcggt acctttgacc agcgcggcgt gataagcgag
      241 caattgcagc ggcacgacgt gcagcagcgg cgagagctcg ccgtagtgtt cggggaggcg
      301 gatgaggtgc acgccctcgg agtcggcatc catatcgcag tcggcgtcgg caaaaatgta
      361 gagttcgccg ccgcgcgcgc ggacttcctg caggttggcc ttgagcttgt cgatcagcgc
      421 atcgttgggg gctacggcga ccactggcat gtccttgtcg atcagcgcca gcgggccatg
      481 cttaagctcg ccggccgcgt aggcctccgc atgaatgtag gagatctcct tgagcttcag
      541 cgccccttcg agggcaattg ggaagtgcat cccgcggccg aggaaaagcg cgtgctgctt
      601 catcgcgaag cgctcggccc agccgcggat cgcttcttcc tgttcgagaa tcttctgcac
      661 agcgaccggc aggtggcgca gcgcgtcgag gtagcgagtt tcggccgcct cgttgagttg
      721 accgcgcagc ttggcgagca cgagcgtcag caggaacagc gcggcgagtt gggtggtgaa
      781 ggccttggtc gaggcgacac cgatttcggc accggctcgg gtgatgaagc gcaggccggc
      841 ttcgcgcacg atcgcggatt cgggaacgtt gcagagcgcc aaggtgtggc gatgactcag
      901 cgccttggca tggcgcagcg ccgccagcgt gtcagcggtt tcacccgact gcgagatggc
      961 gatgaccagt tgccgcgggt tcggcaccga gcgcctgtaa cggtactcgc tggctatctc
     1021 gaccgtgcag ggcaggccgg cgatagcctc aagccagtag cgcgcggtca tgccggcgtg
     1081 gctagaggtg ccgcaggcaa tgatcaggat cgagtcaacc tcgcgcaagg cgatttcggc
     1141 gtcgccaccg aacaggcccg gctggatcga gcgtgcggcg ccgatcattt caagcgtgtt
     1201 ggccagcgcc tcgggttgct cgaagatctc cttctgcatg tagtggcgat agctgccaag
     1261 ctcgatcgcg tcggccgaaa gatgcgactc agtctcctcg cgggtgaccg cggtgccgtc
     1321 agcgagcgtg acgcgccatt ggtccggctt gatctcggcg atgtcgccgt tctcaaggta
     1381 gacgatgcgg cgtgtcacct gcagcagcgc cgaggcgtcg gatgccgcgt agacaccgtc
     1441 ggcggcgagg ccgagcagca ggggcgcgcc ttcccgggcg acgaccaccg tatcggggca
     1501 gtcggtgtgg acgacggcga ttgcgtaggc gccggacagc cgacgagtgg cggcacgcac
     1561 ggccgcgtga agatcggcct cctgcttgag caggtgctct atcaggtgcg caatcgtttc
     1621 ggtatcggtg tcggaggtga agacgtagcc ctgcttggtc agctcctgct tgagcgtcgc
     1681 gtagttttcg atgatgccgt tgtgaaccac ggcgagcttg ccggagatat gcggatgcgc
     1741 attacgctcg gagggaacgc catgggttgc ccagcgcgtg tgggcaatac cggttgcgct
     1801 ttcggtgttg ttgcgctcgg cctgctgcgc gagttcggca acccgcccca cggaacgcag
     1861 gcgttgcagg ccatcaccgg ccgccagcac ggcgatgccg gccgaatcgt aaccacggta
     1921 ttcgagacga cgcagccctt cgatcaggac cgggacgatg ttgcggcgcg ctgccgcagc
     1981 gacgatgcca cacataggtt cagctcactt tttcttctgc ggccgttgcc agccggtaat
     2041 ggaaatctgc ttgacgcgcg agatcgtcag cgattcgggc ggtgcgtcct gcgtcagcgt
     2101 ggtaccggca ccgagcgtgg cgccgcggcc gaccgtcacc ggcgcgacca gttgagtatc
     2161 cgagccgatg aagacgtcgt cctcgatgat cgtgcggaac ttattggcgc cgtcgtagtt
     2221 gcaggtaatg gtgccggcgc cgatgttcac ccggctgccg acggtggtat cgccgacata
     2281 ggcaagatga ttggctttcg agccggcgcc gaggtagctg ttcttcaact cgacgaaatt
     2341 gccgacatgc gattccggac cgagttcggc gccgggccgc agtcgcgcga acggaccgac
     2401 tgtagcgtcg ggaccgatta ccgccgattc ggcgtaggtg aaggcaacga cgcgggcgcc
     2461 ggcggccacc cgcacattgc gcaacacgca atagggtccg atctcggcgc cctcctcgat
     2521 gctcacttcg ccttcgaata cgcagccgac gtcgatggcg acatcgcgcc cgcagcgcaa
     2581 ggtgccgcga acgtcgagtc gcgccgggtc ggcgagccga accccggcct caagcagctg
     2641 ctgggcaatg tcgagctgct ggcatcgctc gagttcggcc agctggacct tgctgttcac
     2701 gccggcaacc tcccagtcgt gcgccggatg gacggtcgtg accggcacgc catcggcgac
     2761 ggcaaggccg atcacatcgg tcaggtaata ctcgccttgc gcgttgtcgg ccgacaggcg
     2821 gccgagccag ccagccagtc gcgccgacgg gatcgccatg atgccggtgt tgatctcacg
     2881 gatcgcgcgt tgatccgacg tcgcgtcctt ctcttcgacg atgccggtga ccttgccggc
     2941 ggcatcgcgg acgatgcgcc cgtagccgcg cggctggtcg agttcgacgg tcagcacggc
     3001 gattccctca ccggccacct gcaggagtcg cgtcagcgtc tcggcgcgga tcagtggcac
     3061 gtcgccgtag agcaccagcg tcgtccccga atccgtgagc agcggcatcg cctgcatcac
     3121 cgcgtgaccg gtaccgagct gaggctcctg cttcgcccac gacaggtcgt cggctgatag
     3181 tgcctcgcgc accgtttcgc cgccgtgacc gtagacgacg cacaatcgat cgggacggag
     3241 actgcgcgcg gcatcgatga cgtgggcggc gagcgccttg ccggccagcg gatgcagaac
     3301 cttgggcaag ttggaatgca tgcgcttgcc ctgaccggca gcgaggatga cgacattgag
     3361 catggcgttg attatccgtt cggaattcgt tttcagcgaa ggaaaaaagc cccgagtcgc
     3421 aacgaatcgg ggctttcacg ggttgccgac ggcggcgtca ccaacgatca acgcggcagc
     3481 gtcgaactgc ccatgaggaa ttcgtcgacg gcccgcgcgc actggcgacc ttcgcggatc
     3541 gcccagacga tcagcgactg gccacggcgc atgtcgccgg cggcgaaaac cttgtcggct
     3601 gccgtactgt aggctttctc gccggcgact tcggcgcgcg cattgccgcg atggtcgcga
     3661 tcgacaccga aggcatcgag cacgctctgc atcggattga cgaaacccat ggcgaggaag
     3721 gcaagatcgg ccttgatctc gaactcgctg cccggcactt cctgcatctt gccgtccttc
     3781 cactcgacgc ggaccacctt gagcgccttg agcttgccgc cctcgccgat gaaggacttg
     3841 gtcgacacgg cccagtcgcg ctcgcaacct tcctcgtgcg aggacgaggt acgcagcttg
     3901 atcggccaat agggccagac gaagggcttg ttctcttcga ccggcggctg cggcatcaac
     3961 tcgaactggg tcaccgacag cgcgccctgg cggttcgagg tgccgacgca gtcggacccg
     4021 gtatcgccgc cgccgatgac gaccacgtgc ttgccggtgg cgagaatctg gtccttgatc
     4081 ttgtcgccgg cgaccacgcg gttctgctgc ggcagaaact ccatcgcgaa gtgcacgccc
     4141 ttgagctcgc ggccggggat agctaggtca cgcggcatct ccgagccgcc ggcgaggaca
     4201 accgcatcga aatcggcaac gagtttctcg gggtcgatcg acgacttggc gaggttgaga
     4261 acgcccggct cacccacccc cttgccgatg aagatgccgg tgcggaaaac cacgccctcg
     4321 gcctccatct gcgcgacgcg ccgatcgata tgcgatttct cgagcttgaa gtcgggaatg
     4381 ccatagcgca gcagcccgcc gatgcggtcg cttttttcaa agaccgtcac ctcgtggccg
     4441 gcacgcgcca gctgctgggc agcggctagg ccggccgggc cggagccgac cacggcaacc
     4501 tgcttgccgg tcttcgattt cggcggctgc ggcacgaccc agcccatctc gaaacccttg
     4561 tcgatgatcg cgtgttcgat cgacttgatg ccgaccggcg cggcgttgat gttgagcgta
     4621 caggccgctt cgcagggcgc cgggcagacc cgtcccgtga agtccgggaa gttgttggtc
     4681 gagtgcagca cgtcaagcgc gtttttccag tcaccgcgat aaacaaggtc gttccagtcc
     4741 ggaatgatgt tgtgcacggg gcagccgtcg ttgcagaacg ggatgccgca atccatgcag
     4801 cgcgcgccct gcaggctcgc ctcctcgtcc gagaggcgca gcacgaactc gcggtaatgc
     4861 ttgacgcgct tgtcggccgg ttcggccgcc tcggtgagac ggtcgtactc gatgaatccg
     4921 gttggcttgc ccatcagtcg atctccaaat ctctgcaacc aagtcgccgc gacatcacgc
     4981 agccaccttg cggtcctttt tctgcatctc ggtcagtgcg cgtcgatatt ccttggggaa
     5041 gaccttgacg aagcgttcgc gcacgttatc ccactgcgcc agcaactgct tggcacgggc
     5101 actgccggta taggtagcgt gccgctcgat caggctcttg agcagggcct cgtcttcctg
     5161 gccgcgatgg cgcggttcgc tgtcgggctg gcgccccgcc ggacggaccg ggtgcagatc
     5221 gaccatcgcc gtgttgcagc gctgcgcgaa ctggccgtcg acatcataga cgtaggcaat
     5281 gccgcccgac atgccagccg cgaagttgcg cccggtctcg ccgagaacga cgacggtacc
     5341 gccggtcatg tactcgcagc cgtggtcgcc aacgccttcg acgaccgccg tcgcgccgga
     5401 gtttcgaaca cagaaccgtt ccccgccgac tcccgagaag aacgcttcgc cttcggtcgc
     5461 gccgtacatc acggtattac cgatgatgat gttctcggtc gcagcaccgc tgaaatccgg
     5521 atgtggccga acgatgatcc gtccacccga aaggcccttg ccgacgtagt cgttgccctc
     5581 accgatcaag tcgaaggtga cgccgcacgc gaggaaggcg gcgaagctct gaccggccgt
     5641 accgcgtagg cggacgtgga tcgtgtcgtc gggcaggccg gcgctgccgt agcgttcggc
     5701 gactcggccc gacagcatgg cgccgacggt gcggttgatg ttcttgatcg tggtgtcgat
     5761 gctgaccttt tcgcccttcg tcagcgccgg ttcggcaagc ttgatcaact gatgatcaag
     5821 cgcccggtcg agaccatggt tctgaagtac ttcctgccgg cgctcggctg gcgaggccgc
     5881 cggcggcatg tagaagatgc gcgtgaaatc cagtccgttc gccttccagt ggtcgatccc
     5941 ggccttcacg tcaagcaggt cggagcgccc gatcaggtct tcgaagcggc gaatccccag
     6001 ctcggccatc agctcgcgga cttcctcggc gacgaagaag aagaaattga cgacatgctc
     6061 gggctgtcca gtaaagcgct tgcgcagctc gggatcctgg gtggcaacgc cgaccgggca
     6121 ggtattgagg tgacacttgc gcatcatgat gcagccctgg accacaagtg gcgcggttgc
     6181 gaagccgaat tcgtcggctc cgagcagcgc gccgatgacc acgtcgcggc cggtcttcat
     6241 ctgcccatcg acctgaacgc ggatgcggtc gcgcaggcgg ttcatcacca gcgtctgctg
     6301 cgtctcggcg aggcccagtt cccagggcgt cccggcatgc ttgatcgacg acagcgggct
     6361 agcgccggtg ccgccatcgt ggccggcgat caccacatgg tcggccttgg ccttcgacac
     6421 cccggcagca acggtgccga cgccgacctc ggacaccagc ttgaccgaca ccgaggcctt
     6481 gggattgacg ttcttcagat cgtggatcag ctgcgccaga tcctcgatcg agtagatgtc
     6541 gtggtgcggc ggcggcgaaa tcaggccgac gccgggaacc gagtggcgca gaaaaccgat
     6601 gtattcggaa accttgtgcc ccggaagctg gccaccttcg ccgggcttcg cgccctgcgc
     6661 catcttgatc tggatctggt cggcgttgac caggtactcg gtagtcaccc cgaagcgacc
     6721 cgaggccacc tgtttgatcg ccgagcgcag ggagtcgccg tcctgcagcg tgtagtcggc
     6781 ctggaccctt ccttcgccga tgacctcgga aagcttggta ccacccttga cggccgggaa
     6841 acggcgggcg tcttcgccgc cttcaccggt attcgacttg ccaccgatgc ggttcatcgc
     6901 gatcgccaga cccgtgtgcg actcggtcga gatggcgccc agcgagatgg cgccggtcgc
     6961 gaaccgcttg acgatttcct tggccggctc gacctcgtcg atcggcaccg gcgccgcggc
     7021 cggacgcagt tcgaaaaggc cgcgcagcgt catgtggcgc ttgccctgct cgttgaccaa
     7081 tttcgcgtat tcgcgatagg tgtggtactg ccccgcgcgc gtcgcgtgct gcagcttggc
     7141 gatcgaatgc ggcgtccaca tgtgctcttc gccgcggata cggtaggcat agtcgccacc
     7201 ggcgtcgagc atggtggcga gcaccggatc gtcggaaaag gcgtcggagt gcagtcgaat
     7261 cgcctcttcg gcaacttcga aaacgccgat accctcgacc ttgctcgccg tgccggcgaa
     7321 atacttggca acgaaatcgg atttgaggcc gacggcctcg aagatctgcg cgccggtata
     7381 cgacatgtag gtcgaaatac ccatcttcga catcaccttc atcaggccct tgccaaccgc
     7441 cttggtgtag ttcttggcgt aggtcgattg cttcgtggcg tcgccgccgg cgagctcggc
     7501 aagcgtctcg aagaccaggt atggatgcac ggcctcggct ccatatccac cgagcagcgc
     7561 gaaatgatgg acttcgcgcg ccgaaccggt ttcgaccacg aggcctgtcg aggtgcgcaa
     7621 accgttcttg accaggtgct ggtgcacggc cgaggttgcg agcagcgccg gaatcgcgac
     7681 gctgtcggcg tccatgcggc ggtcggagac gatgagaatg ttagcacctg atcgcacggc
     7741 gtcttctgct tcggccgcga gcgaggcaag ccgtgcttcg atgccttcct tgccccaggc
     7801 agccgggtag cagatgttca gctcgtgcga gcgaaatttg ccgccggtgt atttctggat
     7861 gtggcggatc ttttcaatat cgccggacga cagcaccggt tgggcgactt cgaggcggat
     7921 cggcggattg atgtcgttga cgcccagcac attcggcttc gggccgatga aggaaaccac
     7981 cgacatcact aggtcttcgc ggatcgggtc gattggcgga ttggtgacct gcgcgaacaa
     8041 ttgcttgaaa tagttgtaga aggtcttggc gcgattcgag agcaccggca gcgcggcgtc
     8101 attgcccatt gagccggtcg gctcctcgcc gttggcgatc atcggctcga tgatgaactt
     8161 gatgtcctcc tgcgtgaaac caaaggcctg ctgccgatca aggagtttcg tcgcaggctg
     8221 ctcgcgcgat tcgtccgcaa ccggtatctc gtcaagcttg atgcgaatct tgtcgatcca
     8281 ttcgcgatag ggcttggcgt tggccagcga gtccttgagt tccttgtcgt cgatgatccg
     8341 cccctgatcc atgtcaatca gaaacatctt gcccggctgc aggcgccact tcttaacgat
     8401 cttcgattcc ggaaccggga gaacgcccga ttcggaggcc atgatcacca ggccgtcatc
     8461 ggtgacgcag tagcgcgcgg gacgcaggcc attgcggtcg agcgtggcac caatctgccg
     8521 tccgtcgctg aaggcgaccg ccgccgggcc gtcccagggc tccatcatcg ccgcgtggta
     8581 ctcgtagaag gcacgccgat tggcgtccat tagcgtgtgc ttttcccatg cctcggggat
     8641 catcagcatc atcgcgtggg ccattgaata gccgcccatc accagcaact cgagggcgtt
     8701 atcgaacgat gccgagtccg actgaccagg atagtgcagc ggccagatct tcttcaggtc
     8761 gtcaccgagc aacggcgagg cgcacgcctg ctcgcgcgcc ttgaaccagt tgacgttgcc
     8821 gcgcagggtg ttgatttcgc cgttgtgggc gatcatccgg aacggatgtg caagatccca
     8881 agtggggaag gtgttggtcg agaaacgctg gtgcacgagg gccagcgccg aaatgcagcg
     8941 cggatccgca agatcagcgt agtactctcc aacctgggtg gcgagcaaga ggcccttgta
     9001 gacgaccgtg cgcgctgaga acgacgccat gtaaaactct tggccgtgtt tcagtttcag
     9061 ggactttatg gcgttggcgc cgcgccgacg cagaacgtag agcttgcgct ccagggcatc
     9121 agtcaccaac acgtcagggc cgcggccaac gaagacctgg cggatgaccg gctcggtctt
     9181 gcgtacggcc ggcgacattg gcatctcgcg attgatcgga acgtcgcgcc aacctagcaa
     9241 cacctgccct tccgcgcgga tcgctcgctc gatctcctcc tgacacgcga gtcgtgaagc
     9301 cgactcgcgc ggtaggaaaa ccatgcccac cccgtattca ccggcgggcg gcaaagtgat
     9361 gccctgtttc gccatttctt cgcggtaaag ggcatcggga atctgaatca ggatgccggc
     9421 accgtcgccc tgcagtggat cggcacctac agcgccccgg tggtcgaggt tcttgaggat
     9481 cagcagaccc tgctcgacga tcgaatggct gcgctcacca cgcatctgcg cgacgaaacc
     9541 gacgccgcag gcgtcgtgtt cgtaggcggg gtcgtagagg ccttgtgttt caggcagcat
     9601 cgggctcatt cgcgtgatcc ttgtcgataa cgttccgggg gtgtcgcgac gcccgaggcg
     9661 gtcttgcttt ttcaagcaaa tcagccgcat aggttgaaaa agccggcttg gccggcttat
     9721 tgtgcgctgc gtttcgggcg gttggcgaat atatcgcgta agccctgttg actcaagcgg
     9781 ttggccgggt cggtagcgcc ccagatcggt gcgttatggc gggtttcgac aactcaggtt
     9841 ccgtcgaccg aaaaaagccg cttgtgctca gccagtgcgt agcggtccgt cataccggag
     9901 acgtagtcgg caacgctgcg cggctgattg tcgtcctgtt ggtattttgg cggcaacagg
     9961 cggggatcgg cgatgaaggc gccgaagagg tcgcgaacca cgcgttgcgc cttgttggtc
    10021 atccgcagta cgcggtaatg gcggtagaga ttctggtgca ggaactgctt gagcttgcga
    10081 tgttccgccc acagcgcgtc gctgaaggca accagcggct cggcgtcacg gacgtcttcg
    10141 agcgttactg gtgcgacggc ttcgagcttg gcggtggtcg ttacgatgag gtcggtcacc
    10201 agttcgttga tcattcggcg aacggtttcg tggacgacgc gacggccggc cagctgcggg
    10261 tacgcattcc ttactgtcgc caggtgacgc gagaacagcg ggacggcttc gagctgcgcg
    10321 atcgtaatca accctgagcg aaggccgtcg tcaacgtcgt ggttgttgta ggcgatctcg
    10381 tcagcgaggt tacacagttg cgcctccagc gacggtcggc gcttggcgat gaagcgctct
    10441 cctacgtcac cgatggttct ggcgtttctt atcgagcaat gcttgacgat tccttcgcgg
    10501 gtctcgaagc aaagattcag cccggcgaat tcggcgtaac ggttttcgag atgatcgacg
    10561 attcgaagcg actgaagatt gtgttcgaag ccgccgtgcc ttctcatgca gtcgtcaagt
    10621 gcttcctggc cggcgtgccc gaacggcgtg tgccccagat cgtgtgccag cgcgatggct
    10681 tccaccgcgt cttcgttcaa tcgcaaagcc cgggcgatcg acctggcgat ctgcgcgact
    10741 tcgatgctgt gggtcaggcg cgtgcggaac aggtcaccct cgtggttcac gaagacttgc
    10801 gtcttgtact caaggcggcg gaaggcagtc gagtggacga tacggtcgcg atcccgctga
    10861 aactgcgagc gcaggttcgg agcggcttcg ctataacggc gcccgcggct gtccgcatcg
    10921 ctgatggcgt agggggctgg atcagccatc ggctggcggc gtccgggcga cgatggcgtc
    10981 gcggacgcga tgcggctcta ggttggccgt gagtaccgcg tcccctattc cccgcaagag
    11041 gacgtactgg atccgcccgt ccttgacctt cttgtcgtgc tgcattaact caaaatagcg
    11101 atcgggggac atcgctggcc cccgggtcgg cagcttggcg gcccgaaaca gggcgtcgat
    11161 ccggtcgaca tcgcctttcg acaaccagcc attccgttgc gagacctcgg cagcgatcat
    11221 cgttccggcc gccactgctt caccatgcag ccacaaacca taacccatac cggtctcgat
    11281 cgcgtggccg aatgtatgac cgaggttaag cagggccctc gagccggcct cgcgctcgtc
    11341 ggccgatacg actgccgcct tgtggctgca agaccgttcg atggcgtagg ccagcgtttc
    11401 ggggtcgcgg cgcaggaggc cgccaatatt ggactcgatc caatcaaaga actcggcgtc
    11461 gcggatcaag ccgtacttga tgacttcggc taagccggcg ctcagctccc gcggcggcaa
    11521 ggtattgagc accgtggtgt cagcgaccac cagacgcggc tgatagaacg cgccgatcat
    11581 gttcttgccc aacggatgat tgatcgcggt cttgccgcca accgaggaat cgacctgggc
    11641 aagcaaggtt gtcggaatct ggataaatgg cacgccgcgc tgatagcacg cggccgcgaa
    11701 tccggttatg tcaccaacca caccgccacc gagagcgatg acggtcgtgc tgcgctcgca
    11761 cgcgtgttca agcagggtgc gaaagattcc ttggagggtt tcccagttct tgtactgctc
    11821 gccgtctgga agatcgatgc gtagagtctt gatgccggcc gcgttcaacg caccggccag
    11881 gcggtcggcg tagagcggcc cgacggttac gttggtaacg atcacggcgc actttccgga
    11941 tatcgcgccg gcgattggtc tcgggtcgtc cagcagcccg gccccaatca atatgcggta
    12001 cgaacgttcg ccgaggtcga tctcaagctc gtgtttcaac acgtaaacaa atcctctttg
    12061 ctgagtcgct cgacgatctg gctggaggtg ttgcgttcga cgtcgatgac gatatcggcg
    12121 atctcttcgt acagaggtcc cctgatgtcg agaagcgacg cgagaaccgc cattggatca
    12181 gcgacctgca gcaagggccg actgcgatcg tgccgcgttc gctggtaaag cgtcttgggg
    12241 gcagctttaa ggtagcagac tgtcccgcag tcgcgaatgg tttgccggtt ttcaggcgac
    12301 agaacagcac cgccaccggt cgctagaatg atattcggtt cccgcaacag ttcggcaatg
    12361 accttggttt cgcgctgccg aaagcccgat tcaccctcga tttcgaagat gacggggata
    12421 gtcacgccgg tgcgattctc tatctcatga tccgaatcga caaaacgcca gcccgtcttg
    12481 cgggccagcg ctttcccgat cgttgttttc cccgcgccca tgaggccaac gaggaccaac
    12541 gcggtgtggt tgcgcggggg aatactggaa ctttactggt ggcagctgcc gcctactgca
    12601 gggtcacctg ttcgttgacg attcgcggcg taatgaagac cagcaactcc gagcgctgtt
    12661 tggaggttgt tcgtgtcttg aaaagccaac ccaatccggg gatatcgcgc agccccggaa
    12721 ttcccgattc gtcgaatcgc tcggactcgc tatagattcc accgatgacc acggtgccac
    12781 cgttgtcgac aaccactttc gtggttacct gtttagtatt gatcgcaaca ccacccggca
    12841 ccacagcacc cggcgaatcc ttgttgacct tgatttccat gccgatccga ccatctgcgt
    12901 tgatcgtcgg cgtcacgtcg aggctaagcg tcgccttctt gaacgagacg ctggtcgcgc
    12961 ccgaactcga ggcctggagg taggggatct cctgaccgtc ctcgatcttt gccgactcgc
    13021 cgttcatggc caatacgcgt gggctggaga tgatctttcc gttgccgtcc gcttcgagtg
    13081 cggtcaattc caagctcagc gttctggtct gggtcgcgtt ggtcagcaga aaattcaggt
    13141 tgccaccggc gaccggtagc gcaacgctgg caccgagctg ctgaaggacc aggttgttct
    13201 ggccggccag gcgcgatgcg tggttgtcgt tgatcccgag tcgtgtgccg agatcacggc
    13261 tgaacgagtc gctggcttcg acgattctgg cctcgatcag tacctgccgc ggcggcaggt
    13321 cgatctcctt gagaacgcgg cggatgtcct caagagtcga ttcgggggcc ttgatgaaga
    13381 gcttgttcga gcccgactcc gacatcagct tgttgccctt agatgccttg ggatcgaact
    13441 tgaggtattc ctccagcttc ttcttggcga catcggcagt cagataattg acttggaaaa
    13501 ccgcatcctg cggcatctca agctcgatcg cctcggtctt gaccttgttg cgagcgatga
    13561 tttcctcacg cggcgcgatc atcacgacgt tgccatcgac gcgcttgtcc agccccttct
    13621 gctgcatgat gatttccagc gcctgatccc acggcacatc ctgaaggttc agcgagatcg
    13681 ttccgccgac tgcttccgag atgacgatat tgaaactggc gaaatcggag aaagcctgca
    13741 ggacctcctt gaccggataa tcccgaaaac ggagggacag gcgctcgccg gtataacgtc
    13801 cagcgctacc caactgtgcc gtctcctgac tgaccggccg cagttcgaca acaaactgat
    13861 tgtccgcctg gtaggcaagg tggtcccagt tgccttttcc ggcgaccacc atccggactc
    13921 ggtccccgcg gcggaaggtg tcgattgaac tgaccggcgt ggagaaatcg gtcacgtcga
    13981 gtttccgtgc gagcgacgcc ggcaggtcgg tcttgctaaa ttcgacgatg aggctgcctc
    14041 cctgcttgcg gatgtcgatc cccgccgtat tggccgtttg caggtcaacg agaacccttg
    14101 cctccccgtc cttgccgcgc cggaaggcga tatcacggat ctgggtaagt gcctgtattg
    14161 caggctgcgc gatggcaaac ggttcggcac tcggcgtcgg cgatgccagc tcgatggccg
    14221 gagccgcgtt catcaggaca atcagatcct tgccgtcgat ctgggtctgg taggcagccg
    14281 ggcgatcgag attgaagacg acgcgcatgc gattccccgc ctgcgcgacg ttgtagctgc
    14341 ggagaaaagc ctgcgtgacc gattgcgcgc cagtctggat cgccgcgtca gtctcggtaa
    14401 agtcaagggc gacccgcgcc ggtttggtca acgaaaaacc gggcggctgg ctggtcagcg
    14461 ggttggcgaa agaaaggcga acgaccacct gatcgccagc tgccgagacg ttgacgtttt
    14521 ccagggtgtt ccgctcttcg gcgtgggcaa tgcctccgat ggccagcgcc gcccacgcgc
    14581 ccaatgcaaa aattgttcgc ttgttcattg tttgcctccg gctccgctca agtgaagttc
    14641 gctgtctcgc tcgacccatg ctccatcggg ttcctggatc acttccttga gcacaatcat
    14701 ttccccgttt tgttcggtaa cgatttgggt tacccggccg aagttctgac cgatgtagtt
    14761 gcctacgcgc aggtcgtagg tcttgttgtt gaccaacacc tgtgcaacca ggcgcttctt
    14821 gtcctgaaag aagccgacga acttcatcgt ctccaacggg aattcttcga gtggctcgcg
    14881 cggacggttg aggtcgggcg ctggcccgcc actccggcca ccgccccctc cggtcgcaat
    14941 tcgaccttgg tcaaacggcg aacgtaatgc gtcggcatca tagagcagcg gttcgtaggg
    15001 tcgaacctgt ggcaggggct tgatatttcc acgcagatct ttcgacgctt cgcgcatcca
    15061 ttggcggagg tcttcgtgtt cgttatcgcc gcaaccaccg acggcaaaca aggcgaccgc
    15121 gagcgccacg atgctcgcga tccggatcgg cgaacgttcg ctatcagatg agcaactcat
    15181 cgccgcctcc ctttttgcgc ctgagttgcc gccctgcgag ctgccgccac ctcttcctca
    15241 tcaagatagc ggaaggtctt tgcgattgcg tccatcgaca aagtctcacc tttgcccgaa
    15301 acgatggtca tgttgttcaa tgtgacgata cgcgacagcc ggccaatgtc ggccgcaaat
    15361 gcgccgagat cgtggtagcc accggttagc ttgagggcga ccggcaattc cgcgtagaac
    15421 tccttcttga tttccgcaga tggcttgaac agctcgaact gcaatccacg tccgaggccc
    15481 gcttgattta tctcgaccag aagggtatcg acctcggtct tgttcgggag ctgcttcagc
    15541 aaggcgccaa acgaggtttc gacttcgttt agctgctgga tataaaggtc caggttgacc
    15601 gcctgcttct tcttgtcgat gaactgctgc ttgagcgtaa tttcctgcgc gcgcgcattc
    15661 tctagtgtaa cgagctggtc attccagacg aaccaccaac cggcggcgat cacggcaacg
    15721 aacacgaccc cgaggacggc cattctcggc gcaaaaggcc aggcaccaat atcccgcgga
    15781 ttaagattct tgaagtcatc ggccagagac ttcagctttt cggtcgaaat cgccggcgct
    15841 ttcatttgcg ccccctttgc ggcgtctgcc gcgcatccgc gtcagcctcg gaagcggtgc
    15901 gggtcacggt tgtcgtcata cggaagtcct gcacccgacg ttccttgcca ctcgcggcct
    15961 tggtttcaat cagctttgga tcctgaaaga ccggcgacga ctcaagattg cgcatgaagg
    16021 ttgaaacgcg ggcgtttgac tgcgacgaac cctccaaact gatgttcgcg cccgtctgct
    16081 taaacgattt aatgaagacc ccatcaggaa tctgtttgac caattcattg agcattgcga
    16141 cgggttcggc acgatcacgc tgcagggatt caatgatttc ctttcgcgcg acctgaaggc
    16201 gaatattggt ctgcaggtcc ttgatctgtg cgatctgctt gtcgagcgag gcgatttcgc
    16261 gctgcaggaa ctggttcttt tcgttctgcg cgccaatgta gccagagatg accgaccagc
    16321 caagaccgac tatcgcggca gcaagaacgg ccattagccc acccaaggca aagaactgct
    16381 gccgcttgaa gcgccgcttc tcttcacgat gcggcagcag gttgatccgg atcattcgtc
    16441 gaacctccgc aacgccaagc cgcaggcgac catcagcgac gcgctgtctg tcaacagatt
    16501 gcgcgcacga acccgatccg acaacaccat gctggcaaac ggattggcga cgaccgtgtt
    16561 cacctgaacg cgtgcggcaa ctgcttcgtc ggcccccgcc aacgcaccag tgccgccggc
    16621 cagaactatg tggtcgacct gattgaactg cgtcgatgtg aagaagaact gcaatgcccg
    16681 cgaaacttca agcgccagat catcaatgaa cggtcgcaag agatcggatt cgtaattttc
    16741 cgggagattt cccgatcgct tcgccgcctc ggcctcttcg aaactcattc cgtactggcg
    16801 cgcgatgttc tgcgtcagtt gcgtgccgcc gaaggcctgc tcacgggtat agaccggctg
    16861 gagattacgc aggacagtca ggttcatgac actaccgccg atatcgacca gcgcgacaac
    16921 ttgattgtca cccgcttccg gtagctgggt ctgaaccagt tcaaacgccg ccaacagtgc
    16981 atgcgactcg acatccatta cgctggcggt caggccggcg gcctcagcca cggcgacacg
    17041 gtcttcgacc ttttccttgc gagatgcggc gatcagaacc tcgacctcat cgggactcga
    17101 cgctgcgggg ccgatgacct ggaaatccag attgacctct tcaagcgcga agggaatgta
    17161 ctggttggcc tcggactcga cctgcacctc gagctcctct tcgcgctgcc cggccgggac
    17221 aatgaccttc ttggtaatca ccgaggcagc gggaagcgcc attgcaaccg agcgcgtgga
    17281 cgaaccgagc cgtttccagg cgcgcttgac cgtttcggca gctacctcaa ggttcgcgac
    17341 gttgccatcg acaatcacat ctttcggcaa cacttcaaca acatagcgct caacgcgata
    17401 gccgtttcga ctatcgaaag acagctccac catcttgacc gcagacgaac tcaggtcgag
    17461 accaatgagc ggcttggctt tcgcgctaaa tatcgacagg tcgatctgca agcaactctc
    17521 ccgaaaaaag tcttttactg cgggatgtta tgagacacac acataatcca ctttaaatcc
    17581 taacaacaag gtaacggtat gtaaagtgat ttttgcagtc gcacatgggc aactataatc
    17641 cgcaactttc gtccgaaagc gatcccgttg aaagaacaaa cacgccgttg gctgctcgtt
    17701 tccctgtatg cgctgggaac tgcatttcta agctttacgg cactggtcgg gattgccgtt
    17761 ctcaaggcgc aatcgcagct cccgacgctg gaagccgtca ccgattaccg gccgcgaatt
    17821 cccctgcggg tctataccgc cgacggcgac ctgatcggcg agttcggcga ggaacggcgc
    17881 agctttgtaa ccattgacaa gatcccaaac gtcctgcggc aggcgatcat cgcggccgag
    17941 gatgagcgtt tctacgcgca tcgtggaatc gacgtgctcg gtgtcatgcg tgccgcgtac
    18001 gccaacctga ttcgcggtag ccgccgccag ggtgcctcga ccatcaccat gcaggtcgcg
    18061 cgaaatttct tcctatcaac cgaaaagaca ctcaaccgaa agctgtttga ggccttgctg
    18121 gcgattcgca tcgagaacaa tctttccaag gaccagatcc ttgagttgta cgtaaaccag
    18181 atctacctcg ggcagcgcgc ctacggtttc ggcgcagcgt cccaagtgta tttcggcaag
    18241 acagtgtcgg acttgaatct gggagaagcg gctgtccttg caggcctacc aaaggccccg
    18301 tcggcctaca acccgatcgc caacctcaag cgggccaaac aacgacaaga atacgttctg
    18361 cgtcgcatgc acgagcttgg cgcaatcgac gattcccagc tggccgatgc aatcgccgct
    18421 ccaatcgtga cgcgcaagga aacgacgctg gtcagctacg agacaccggc cgaccatgcc
    18481 gccgagatgg cgcgccagat tgccatcgag cgcttcggcg aggacgtcta cacgcgcggc
    18541 tacaaggtaa tcacgacctt gcgcacgcgc gaccagcgcg ccgcacgcgc cgcgctggaa
    18601 aatggcgtgc gggcctacga ccgtcgccac ggctatcgcg ggccggaggc ctttcttccg
    18661 ttcgacgcca agcagcggcc gaccgacgag gaactcgaag aggcgctggg cgagttctcc
    18721 gacagcgcag atctgtcgct ggcggtcatt acggagatcg acaagcgcaa ggtaacggtc
    18781 tatcgccggg gcggcgagac cctgacgctc agcgacggcg gcctgaagtt cgcggccgag
    18841 gcgctcgatc ggcgcgcgcc ggcacgcatg cgtttccgtc agggagcggt cgtccggctc
    18901 gcgaggatga ccgacggcga ctgggagata tcgcaggttc ccgatgtcga ggccgcgctg
    18961 gtcgccgccg atccatcaac cggggcgatc cgggcgctgg tcggcggtta cgatttcggc
    19021 cgcaaccagt tcaatcacgt gacgcaagcc atgcggcagc cgggttcgag cttcaagccc
    19081 ttcatctact cggcggcgct cgaacgaggc tatacaccgg cctcggtgct cgatgacacg
    19141 ccagttctcc taccgccgga agagaccggc ggacaacctt gggagccgaa gaattacgac
    19201 gacctctacg acgatccgat gcggctgcga atcgctctgg cgaagtcgaa gaacatggtg
    19261 gctattcgcc tgttgcaggc gatcacgccg gcgtacgcac gcgactttgc ggcgcgcttc
    19321 ggcatcgagc cggcaaacaa cccggcctac ctgacaatgg cgctcggctc gggcacggcg
    19381 acgccatggc agatggtcgg cgcgtattcg gtgttcgcca atggtggcta tctggtgatg
    19441 ccttacctcg tcgatgagat ccgtgacgaa aacaatcagg tcatcgcccg cttcgaaccg
    19501 cgtcgcgccg gcaccgaaga tctgcgggcg atcgacccgc gcaatgcctg gacaatggat
    19561 tcgatgttac gcgatgttgt cgcctacggc accgcggtcg gcgccggaag ggcgctgaaa
    19621 cggctcgact tggccggcaa gaccggcacg acaaatgaca acgtcgatgc ctggtttacc
    19681 ggctaccacc gcagcgtggt ggccgtgtcc tggattggct tcgaccagcc caaacagctt
    19741 ggctcagggg aaaccggcgg tcgggccgcg ctgccgatct ggatcgacta catgcgcgaa
    19801 gcactcgccg acacgccgat cgaaaccgat ccgccaccac cgcctcccgg catgtcgctg
    19861 atccaggcga tcgacctgtc tgttcccgaa ggcgtcatcg acgaatgggt ctacgacgaa
    19921 aacgtgccgc ccgagccgcc ggtgccaagc ctttggtcgc ccttcgaccc gttgctcccc
    19981 ggcaactgac cggcggagcg cgactgacct attcgagcgc aaccggctgc tgcgcctgga
    20041 ggctgactag gcgcgacagc gcggcgacca atcgttcgcc ggatcgcgta tcgacccagt
    20101 ccgtcccatc gaagcgaaaa tgaaagcctc ccgagcgcgc cgcaacccat atttccctag
    20161 cggcggcgtg acggttgatg atcaactgcg agccgtcggg gaactcaact tccagtaccc
    20221 cccccgccaa ttggttgaga tcgagatcga taccgcagct ctctagcgcc ccgtcgatcc
    20281 actcaagggt ttcgtttgcc cgctgatcga attctttgtc gtccatcgtc atagccgtta
    20341 tcctcacgcc gcgaaaccgg cacggaaacc tatcctaaac gaaccaccat gcgtagacac
    20401 acgatctgtt cggcgcttct gcttctcctg ctcgccgcct gcggcaccaa gggtccgctg
    20461 gtcctgcctg ccaccgcggt ttacgcgcca ccaaccacca ctggaacatc gcgatgagct
    20521 tcactttgat cgatggggaa ctgcacgccg agcaattgcc gctgtcggaa attgccgccc
    20581 gtttcggaac gccctgctac gtgtactcgc ggcaggcgct gacttcggcc ttgcaggaat
    20641 ttcagtcggc gctcgagggc accgattcgc ttgtctgtta tgccgtcaag gcgaacagca
    20701 atctggcaat tctcaatctt tttgcgcggc agggcgccgg atttgacatt gtctcgctgg
    20761 gtgaactcac gcgcgtcatt gccgccggcg ccgatccggg aaaggtgatc ttctccggcg
    20821 tcggcaagcg ctccgacgag atcgaggctg ccatcatggc cggaatcggc tgcttcaacg
    20881 tcgaatccga acccgagctg tcacgaatcg acgagatcgc cacacgactg gggaagaagg
    20941 cgccaatcag cctgcgcgtc aatcccgacg tcgatccgaa aacgcatccc tacatctcga
    21001 ccggcctgcg cgagaacaag ttcggcgtcg actggcagtc ggcccgccgg atctatcagc
    21061 acgcggcgag cctgcgtggt ctggaggtgg tcgggatcga ttgccacatc ggctcgcaga
    21121 tcctcgacgt cgcgcccttc ctcgaggcgc tggaacgaat cctcgatctc atcgaccagc
    21181 tcgccgccga cggaatcgta ctgcgccaca tcgacctcgg cggcggcctg ggcattcgct
    21241 atcgcgacga aacggcgccg gcgatagccg actacctcgg cgcggtgcgc gaccggctcg
    21301 ccggccgcaa actcaagctg ctgctcgaac ccgggcggcg cctggttggc aatgcgggag
    21361 tactgctgac acgcgtcgaa tacattaagt ctgggccaga acacaatttc gcaattgtcg
    21421 acgcggccat gaacgacctg ctgcgaccgg cgctctacga tgcctggcac gacatcgtcc
    21481 cggtgcgcac gcgccccggc gaaagccggc gttatgacgt ggtcggcccg gtctgcgagt
    21541 cgggggactt cctggcgaag gaccgcagcc tgtccatcgc cgccggcgac tgcctcgctg
    21601 cgatgtcggc tggcgcctac ggcatgacca tgagttcgaa ctacaacacg cggccacgcg
    21661 ccgccgaggt gatggtcgac ggcagcgtcg cccacctaat acgccggcgc gaatcgattg
    21721 ccgagctcta cgcccaggaa acggtgctgc cggcctaagc cggccaacaa tccctcgcgc
    21781 tgccgatcaa agcaccagcg cgagcgccag cggcacggca atcagcgcgc cgagattgcc
    21841 gaccatgacg atcgacgcca cgcgctgcgg ctcctggtga tagcgctcgg cgaagacgta
    21901 attgagcacg gccggcggca acgcgccaaa aacgatcagc atgtcacgct gaattccttc
    21961 gattcccagc agcagcgccg cggcgtaggc gatcaacact ccagagagcg ggcagaagac
    22021 cgccccgatc aggccgatcc gcaggtcctg gaacgccgag tcggtcaggc ggacacccag
    22081 cgagaacagc agcagcggaa tcgagacatc accgagcatg cgcacggcgg tcaggaacgg
    22141 tggccacggc tgaaatcccg acaggctcac cgccaaccca gcgatcgcgg cgagaattac
    22201 cggaatccgc cagagcgtgg tcagcctcgc ccgatggtcg agcatccagg tgccgagcga
    22261 gtaatgcagc acgttctcaa ccaggaaaag catcactgcc gccggcagca tcgcttcgcc
    22321 gaaggccagc atcatcagtg gcaggcccat gttccctgag ttgttgaaca tcatcggcgg
    22381 aacgaaggta tt
//
LOCUS       DATDAA010000455         5959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_173673,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000455 DATDAA010000000
VERSION     DATDAA010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173673"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1539)
                     /gene="nusA"
                     /locus_tag="VIS73_13770"
     CDS             complement(64..1539)
                     /gene="nusA"
                     /locus_tag="VIS73_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY5764272.1"
                     /translation="MSHEMLLLVDALAREKNVAKDTVFGALELALASATKKRINEEAD
                     VRVAVDRENGSFEAFRRWQVVTDEEFENPATQIIIEDAKVQDAEAELGDFLEESLEPV
                     DFGRIGAQAAKQVILQKIRDAEREQILADFLDRKEHVVTGQVKRIDRGNAIIEVGKME
                     AVLPRDQMIPKENLRVGDRVKGFLLRIERTMRGPQLILSRTAPDFIIKLFEIEVPEID
                     DGLMEIKAAARDPGMRAKIAVKSNDARIDPIGTCVGLRGMRVQAVTGELAGERVDIVH
                     WSADPAQFVVGALAPAEVSSILVDEEKHSMDVVVDESNLAIAIGRGGQNVRLASELTG
                     WTINLMSEDEKERKQEAEVAATRVLFMEKLDVDEEVANILIEEGFSTLEEVAYVPMAE
                     MLEIESFDEQIVQELRDRARNVLLTEAIATEENLDQVSEDLLSLDGMDKGLAAKLAEN
                     GVKTREDLADLAVDELVEMSGIESERAKSLIMTARSHWFEE"
     gene            complement(1558..1995)
                     /gene="rimP"
                     /locus_tag="VIS73_13775"
     CDS             complement(1558..1995)
                     /gene="rimP"
                     /locus_tag="VIS73_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY5764273.1"
                     /translation="MDLRLLLETTVVGLGFEVVDIEMSPRAKLLRVLIDSDRAGGVNV
                     EDCAKVSNQLTRVLMVEEIDYDRLEVSSPGMDRPLTKPEHFQRFIGHQAQIRVRIPVA
                     GRRNFAGTLKQVVDGRVCLLVEKNEVEIEFASIEKARLIPEFD"
     gene            2254..2679
                     /gene="dksA"
                     /locus_tag="VIS73_13780"
     CDS             2254..2679
                     /gene="dksA"
                     /locus_tag="VIS73_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEY5764274.1"
                     /translation="MAEDLLHKHFKPYAPKKGEEYMNAKQLAHFRIILETLKKELSED
                     IDRTVHTMQDEATVFADPNDRASQETDIALELRNRDRERKLIKKIDETLGRITSEDYG
                     FCDKCGVEIGIKRLEARPTATLCIDCKELEEMRERQVAK"
     gene            complement(2780..4897)
                     /gene="pnp"
                     /locus_tag="VIS73_13785"
     CDS             complement(2780..4897)
                     /gene="pnp"
                     /locus_tag="VIS73_13785"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY5764275.1"
                     /translation="MFNIAKRSFQYGASTVTIETGEVARQAGGAVMVSIEDTVVLVTV
                     VAAKTAKAGQDFFPLTVDYQEKTYAAGKIPGGFFKREGRPSEKEILTSRLIDRPVRPL
                     FPEGFYNEVQIVATVMSLNPEIDPDIPALIGASAALSISGVPFNGPIGAARVGYINGE
                     YVLCPTLSQLKDSQLDLVVAGTEAAVLMVESEAQQLSEEVMLGAVVFGHEQMKAAINA
                     INELVEEAGKPEWDWQPAPKNEPLIAKVRELAFADLEAAYNITIKQERSQAVKQIVSR
                     VVSTLTEGVDNAPDAGEIKDLLFAMEAEIVRGRILSGAPRIDGRDTRTVRPISVRNGV
                     LPRAHGSALFTRGETQALVVATLGTGRDEQIIDALAGEYRDRFMLHYNMPPYATGECG
                     RVGTPKRREIGHGRLAKRALLAVLPAQDDFNYSMRVVSEITESNGSSSMASVCGGSLA
                     LMDAGVPLKAHVAGIAMGLIKDGNRFAVLTDILGDEDHLGDMDFKVAGTDAGVTALQM
                     DIKIQGITKEIMQVALAQASEARLHILGIMKEAMSGARAEVSSFAPRLYTMKINPEKI
                     RDVIGKGGAVIRALTEETGCTIDIQDDGSITIASVSSEGAEAAKSRIEDIVAEVEVGR
                     IYEGTVLKLLDFGAIVSILPGKDGLLHISQIANERVNAVADYLKDGQKVRVKVLEADE
                     KGRVRLSMKAVSAEEASAEAPQG"
     gene            complement(5091..5360)
                     /gene="rpsO"
                     /locus_tag="VIS73_13790"
     CDS             complement(5091..5360)
                     /gene="rpsO"
                     /locus_tag="VIS73_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY5764276.1"
                     /translation="MAMNAEQRAQIVADNARGKGDTGSPEVQVALLTARINDLTGHFK
                     EHAKDHHSRRGLLRMVSQRRKLLDYLKGKDVERYRSLIDRLGLRK"
     gene            5594..>5959
                     /locus_tag="VIS73_13795"
     CDS             5594..>5959
                     /locus_tag="VIS73_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY5764277.1"
                     /translation="MIRPKPQPPTPRNGERLRLGALHGSSDALVLAAYADEARTHLVL
                     TATAEDARRLATEVGWLAPERRIRLLPDWETLPYDGFSPHQDLVSERLATLYALTQSD
                     CDLIIAPITTAVVRLPPASY"
BASE COUNT         1128 a         1979 c         1722 g         1130 t
ORIGIN      
        1 ggcgggcatc ttcagttcat cggcgaattg tgaaacgctg gtactcgaca tggccttacc
       61 ccgttattct tcgaaccaat gcgaacgcgc cgtcatgatc aggctcttcg cacgttcgct
      121 ttcaatgccg ctcatctcga ccagttcgtc gactgccaga tcggccagat cttcccgcgt
      181 cttgacgccg ttctcggcca gcttggcagc gagacccttg tccatgccgt cgagcgatag
      241 gagatcctcg ctgacctggt cgaggttttc ctcggtggca atcgcctcgg tcagcaacac
      301 gttgcgggca cggtcgcgca gctcctgaac gatctgttcg tcgaaggact cgatctcgag
      361 catttccgcc atcggcacgt aagccacttc ctcgagcgtc gagaaacctt cttcgatcag
      421 gatattggcg acttcctcgt cgacgtcgag cttctccatg aagagcacgc gggttgcggc
      481 gacttccgct tcctgcttgc gttccttctc gtcctcgctc atcaggttga tcgtccagcc
      541 ggtcagctcc gatgccaggc gaacgttctg tccgccgcgg ccgatcgcga tcgccaggtt
      601 gctctcatcg acgaccacgt ccatcgaatg tttctcttcg tcgaccaaaa tcgacgagac
      661 ttcggccggg gccagcgcgc cgaccacgaa ctgggccgga tcggctgacc agtggacgat
      721 atcgacacgc tcgccggcca actcgccggt aacggcctgc acgcgcatgc ctcgcaggcc
      781 gacgcaggtt ccgatcgggt cgatacgcgc atcgttggac ttgaccgcga tcttggcgcg
      841 catgcccggg tcacgggcag ccgccttaat ctccatcaga ccgtcgtcga tctcaggcac
      901 ttcgatctcg aacagcttga tgatgaaatc aggcgccgtg cgcgacagaa tcagctgcgg
      961 gccgcgcatc gtgcgctcga ttcgcagcag gaagcccttg acgcgatcac cgacccgcag
     1021 gttctccttg gggatcatct ggtcgcgcgg gaggacggct tccatcttgc cgacctcgat
     1081 gatcgcgttg ccgcgatcga tgcgcttgac ctgaccggta accacatgtt ccttgcggtc
     1141 gaggaagtcg gcgaggatct gttcgcgttc ggcgtcgcga attttctgca ggatcacctg
     1201 cttcgcggcc tgggcgccga tccggccgaa atcaaccggc tcgagggatt cttcaaggaa
     1261 atcgccaagt tcggcctcgg cgtcctgcac cttggcgtcc tcgatgatga tctgggtcgc
     1321 cgggttctcg aactcctcgt cggtcaccac ctgccaacga cgaaacgcct cgaacgaacc
     1381 gttctcgcga tcgaccgcga cccggacatc ggcttcttca ttgatgcgct tcttggtcgc
     1441 cgaggccagc gccagctcga gcgcgccgaa cacggtgtcc ttggccacgt tcttttcccg
     1501 ggccagggca tccacgagca acagcatttc gtgactcatc caaactctcc taccagatca
     1561 atcgaactca ggaatcaaac gcgccttctc gatactggcg aattcaattt ccacttcgtt
     1621 tttttccacc agcaggcaaa ccctgccgtc cacaacctgt ttcagcgtcc cggcgaagtt
     1681 gcggcggccg gcaaccggaa tccgcacgcg aatctgcgcc tgatgaccaa taaaccgctg
     1741 gaagtgctcg ggcttggtca gcggcctgtc catgcccggc gaggaaacct ccagtcggtc
     1801 gtaatcgatt tcttccacca tcagcacccg cgtcaactgg ttgctgacct tcgcgcaatc
     1861 ctcgacgttg accccgccag cccggtcgct gtcgatcaac acgcgaagca acttggcgcg
     1921 cggcgacatc tcgatgtcga ccacctcgaa tccaagtccg accaccgtcg tttcgagcaa
     1981 taaacgcaaa tccatggcgc acaaataaaa aatgggcgaa acgcccatcg gtaaattttc
     2041 cgagccgttt ccgcagcaag cgccggaaac gaggcgcgca ttctagcgcg attgccagcc
     2101 gcgagcaatg cttgagcacc atcgcagcac atgccccggg cccaattgat cgaccaccga
     2161 ccgagccccg cgcaacttcg ctgcaactta ccttgcgacg gccaagggtt tctgttaaaa
     2221 atccgccttc gctaaatcac cggctgacgg cacatggctg aagaccttct tcacaagcac
     2281 ttcaaaccct acgcaccgaa aaagggcgag gagtacatga acgccaagca actggcgcac
     2341 ttccggatca tcctcgaaac gctcaagaag gagctttcag aggacattga ccgcacggtc
     2401 cacacgatgc aggacgaagc aacggtcttc gccgacccca acgaccgggc cagccaggaa
     2461 accgacattg cgctcgagct gcgcaaccgc gaccgcgaac gcaagctgat caagaagatc
     2521 gacgaaaccc tcggccgcat cacctccgag gactacggct tctgcgacaa gtgcggcgtc
     2581 gagatcggta tcaagcgact cgaggcgagg ccaaccgcaa cgctgtgcat cgactgcaag
     2641 gaactcgagg aaatgcgcga gaggcaggtc gccaagtaac tcacgctgcg caagccgaca
     2701 cccaagagaa agggcgcctt gcggcgccct ttggcttttc ggagggcgac gacaaaggcc
     2761 gcccccgaat atcggccgat cagccctgcg gcgcctcggc gctggcttcc tcggcggaga
     2821 ccgccttcat cgacaggcga acacggccct tctcgtcggc ctcgaggacc ttgacgcgaa
     2881 ccttctgccc atccttgagg tagtcggcaa cggcgttgac gcgctcgttg gcgatctgcg
     2941 agatgtgcag caggccatcc ttgcccggga gaatgctgac gatcgcgcca aaatcgagca
     3001 gcttgagcac cgtgccttcg tagatgcggc cgacttcgac ctcggcgacg atgtcctcga
     3061 tccggctctt cgccgcttcg gcaccttcgg aactgaccga ggcaatggtg atcgagccgt
     3121 cgtcctggat atcgatcgtg caaccggtct cttcggtcag ggcccgaatc acggcgccgc
     3181 ccttgccgat cacgtcgcgg atcttctcgg ggttgatctt catcgtgtag agacgcggcg
     3241 cgaacgacga cacctcggca cgcgcacccg acatcgcctc tttcatgatg ccgagaatgt
     3301 gcagacgggc ctcgctcgcc tgcgccagcg cgacctgcat gatttccttg gtgattccct
     3361 ggatcttaat gtccatctgc agcgcggtga cgcctgcgtc agtaccggca accttgaagt
     3421 ccatgtcgcc gaggtgatcc tcgtcgccga ggatgtcggt caacacggcg aagcgattgc
     3481 catccttgat cagtcccatg gcgatgccgg caacgtgtgc tttgagcggt acgccggcat
     3541 ccatcagcgc cagcgatccg ccgcagaccg aagccatcga cgaggagccg ttcgattcgg
     3601 tgatctccga gaccacacgc atcgagtagt tgaagtcgtc ctgcgccggc agcacggcca
     3661 gcagcgcgcg cttggccaga cgcccgtgtc cgatttcgcg gcgcttcggt gttccgacac
     3721 ggccgcattc gccggtggcg tacggcggca tgttgtagtg gagcatgaag cggtcgcggt
     3781 actcgccagc gagcgcatcg atgatctgct catcacggcc ggtacccagc gtcgcgacga
     3841 ccagtgcctg cgtctcgcca cgcgtgaaca gcgccgaacc gtgggcgcgc ggcaggactc
     3901 cgttgcggac cgaaatcggc cgcaccgtgc gcgtgtcgcg accgtcgatg cgcggcgcgc
     3961 ccgagagaat acggccgcga acgatctcgg cttccatcgc gaacagcaga tccttgattt
     4021 caccggcgtc aggcgcattg tcgacgccct cggtcagcgt cgagacgacg cgggaaacga
     4081 tctgcttgac cgcttggctg cgctcctgct tgatcgtgat gttgtaggca gcttcgaggt
     4141 cggcgaaggc gagctcgcga accttggcga tcagcggctc gttcttcggc gccggctgcc
     4201 agtcccactc gggcttgcct gcctcttcga ccagctcgtt gatggcgttg atcgcggcct
     4261 tcatctgctc gtggccgaag acgacggcac cgagcataac ttcctcggac agctgctgcg
     4321 cttccgactc aaccatcagc acggcggctt cggtaccggc gacgacgagg tcaagctgcg
     4381 agtccttgag ctgcgacagc gtcgggcaca gcacgtattc gccgttgatg tagccaacac
     4441 gcgccgcgcc gatcggacca ttgaacggca cgccggaaat cgacaaggcc gccgaggcgc
     4501 cgatcagcgc cgggatgtcg gggtcgatct cggggttcag cgacatgacc gtcgcgacga
     4561 tctgcacttc gttgtagaag ccctcgggaa acaacgggcg gaccggacga tcgatcagcc
     4621 gcgaggtgag tatctccttt tccgacgggc ggccttcacg cttgaagaaa ccgccgggga
     4681 ttttgccggc ggcgtaggtc ttttcctggt agtcgacggt cagcgggaaa aaatcctggc
     4741 cagccttggc ggtcttggcg gcgacgacgg tcaccagcac gaccgtgtct tcgatcgaca
     4801 ccatgaccgc accgccggcc tgtcgggcaa cttcgccggt ttcgatggtg acggtgctgg
     4861 caccgtactg gaagctacgc tttgcaatat tgaacatttc agtctttctt tcctgtggcg
     4921 ctggcgggtc cccgattccg acctgccgaa ctaactgcta acgccgattc ccaaaacaaa
     4981 agcagcggtg cgacctgccg gtcgaccgcc gccccatgtc gagacaaccg attaggcacc
     5041 cccatcgggc gccaaatcgg gcacgcaagc gcgcgctcgt ttgccaaaca ctatttgcgc
     5101 aagcccagac ggtcgatcag cgaacgatag cgctcgacgt ccttgccttt gaggtaatcc
     5161 agcagcttgc gccgctggct caccatgcgc agaagaccgc gacgggagtg atgatccttc
     5221 gcgtgctcct tgaaatgacc ggtcaggtcg ttgatgcgtg ccgtcagcag cgccacctga
     5281 acttcgggag aacccgtatc gcctttgccg cgggcattgt cggcaacaat ctgagccctt
     5341 tgttcggcgt tcatcgccat ttcaaactcc ttttaggtat tgccggcgca gccccggttt
     5401 gatagagccc acgccagatc aggatgcggc gccccatcgc tgaaaggcac cgccacaacc
     5461 gcttcgacag gaacgcagag atccccggcc aaacggaacg cggattgtac gcaagtcggc
     5521 aatcgcccgc aatgcggaat gccggccggg acctgcctag cgctataatt cgcggctttt
     5581 ttcggctgcc ccggtgattc gccccaaacc ccaacccccg acgccccgca acggcgagcg
     5641 cctgcgcctc ggcgccctgc atggcagcag cgatgcgctg gtgctggccg cctacgccga
     5701 tgaggctcga acgcatctgg tgcttacggc cacggccgaa gacgcccggc ggctggcgac
     5761 cgaagtcggc tggctggcgc cggaacgccg gatccgcctg ctgccggatt gggaaaccct
     5821 gccctacgac ggcttttcgc cgcatcagga tctggtctcg gaacgcctcg cgacgctcta
     5881 cgcgctgacc cagagcgact gcgacctgat catcgcgccg attaccacgg ccgtcgtccg
     5941 cctgccgccg gccagttac
//
LOCUS       DATDAA010000456         3235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_208797,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000456 DATDAA010000000
VERSION     DATDAA010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208797"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="VIS73_13800"
     CDS             complement(<1..1182)
                     /locus_tag="VIS73_13800"
                     /inference="COORDINATES: protein motif:HMM:NF013604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEY5764278.1"
                     /translation="MLKSNMTIKLAVTVLTLALSSAAWSAPGNGRAGLVALQGAAAKG
                     YEVPADVRLARASNLKARGLISHRYQQLVGDAEVLGGQLTVLRSAAGDVIAVTGAHYP
                     GLMSTNDVRLNGNSARGIAAAQIGGAGLWKTRLMINPNDGRYFYIVENRRFDSRWFHW
                     VDAENGSIVNAYDGLTTGSGTGVQGDTKDLTGLTKPNGTRYELMSPEADEQGKPKPRQ
                     VTYDAGNANSPRSLPGTLATDDNDTWDTSGTKSPGQPALVDAHFFANVTDDYFLSEHS
                     FDWLDDYPQGMVSSAHVKRNYNNAYWNGSQMAYGDGDGSAFVELSGDLDVVGHELSHG
                     VTEATSNLIYQNESGALNEAFSDIMGTSIEYYHGTGNWTIGEDITPNDNGIRNMANPN
                     ED"
     gene            complement(1484..2176)
                     /locus_tag="VIS73_13805"
     CDS             complement(1484..2176)
                     /locus_tag="VIS73_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEY5764279.1"
                     /translation="MVRHHERHYAERIGWLRAAVLGANDGIVSTASLLVGVAAAGAAQ
                     GDVLLAGIAGLVAGAMSMAAGEYVSVSSQSDTEKADLERERRELVDNEAFERRELAAI
                     YVSRGLDNALAQQVAEQLMEHDALGAHARDELGLSEIHTARPIQAAVASAITFAVGAA
                     LPLAIAWLSPLTSLVPFVSGGSLLFLAVMGAMASRVGGANIAVGAGRVVLWGALAMAA
                     TAAVGSLFGTVV"
     gene            complement(2533..2949)
                     /locus_tag="VIS73_13810"
     CDS             complement(2533..2949)
                     /locus_tag="VIS73_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764280.1"
                     /translation="MNGKNLVIGSLVGLVALYILGYILFDTVFADFYAQNAGSATGVA
                     REEPLIWATVLGTLSYAVLLTLGIGSRSGAVSAADGAKVGATIGFLLWFTVDFILFGY
                     QNVSNLTLAVTDPVLELVRGGIAGAVIAVVLGKTSG"
BASE COUNT          604 a         1062 c          952 g          617 t
ORIGIN      
        1 atcttcgttc gggttggcca tgttgcggat gccgttgtca ttcggcgtga tatcctcgcc
       61 gatggtccag ttaccggtcc catggtagta ctcgatgctg gttcccatga tgtcggagaa
      121 ggcttcgttc agtgcgcccg actcattctg gtaaatcagg ttgctagtcg cctcggtgac
      181 gccgtgactc agttcatggc cgaccacgtc caggtcgcct gacagctcga caaaagccga
      241 accatcgccg tccccgtagg ccatctggct accgttccag taggcgttat tgtagttgcg
      301 cttgacgtgc gcggacgaaa ccatgccctg cgggtaatca tccagccagt cgaagctatg
      361 ctcactcaag aaataatcgt cggtgacatt ggcgaagaaa tgcgcgtcca cgagcgccgg
      421 ttggcccggt gacttggtgc ctgacgtatc ccaggtatcg ttatcatccg tcgccagcgt
      481 cccgggcagg cttcgtgggc tattggcgtt gccggcatcg taagtgacct ggcggggttt
      541 cggcttccct tgctcatcgg cctcgggaga catcaattca taccgagtgc cgttaggttt
      601 cgtcaggccg gtcaggtctt ttgtatcacc ctgcacacct gtcccactgc ccgtggtcag
      661 cccatcgtag gcgtttacga tcgaaccgtt ctctgcatcg acccagtgaa accagcggct
      721 gtcgaatcgc cggttttcga caatataaaa ataacggccg tcattcgggt taatcatcag
      781 gcgtgtcttc cacaggcctg caccgccgat ctgtgccgcg gctatccctc gcgcggaatt
      841 accattgagc ctgacgtcgt tggtcgacat caatcccggg tagtgggcgc cggtaaccgc
      901 aatcacgtcg ccggccgcag agcgcagcac cgtgagctga cccccgagca cctcggcgtc
      961 accgaccagc tgctgatagc ggtgcgagat gagtccgcgc gccttgaggt tgctcgcacg
     1021 tgccaggcga acatccgcag gcacttcgta acccttggcc gcagccccct gcagcgcaac
     1081 aagtcctgcg cgaccgttgc cgggtgcgga ccacgcagcg ctcgacaggg ccagagtcag
     1141 aacggtaacg gcaagcttga tcgtcatatt acttttcagc atcagactgc acctgatcgg
     1201 attatcatta gatttgtcat tttatgagta ttaaagctac caagccgagc ggggaacagt
     1261 atagcgtttt cgacagcgag ttgccgtgcg cacttggact cagatcgcca atggggaaag
     1321 gttcccaccc tatgccgctt atccagcgag ccgccgcggt acccgatagg tctgcctgac
     1381 cggcggcaac ccgcatcgtg tcggggatcc actggccgtc ggccccagcc cgaaccggtc
     1441 cgtccgagct gcgcatcgaa gcgctggagc ttgccctgac gcatcagacc acggtgccga
     1501 acagcgaccc caccgcggcc gtggctgcca ttgcaagcgc gccccagaga accactcggc
     1561 cggcgcccac cgcgatgttc gccccgccca cacgcgatgc cattgcaccc atcaccgcga
     1621 ggaaaagcag cgaccccccg gacacgaacg gcacgagcga agtcagcggg ctgagccatg
     1681 cgatcgccag cggcaaagcc gcgccgacgg caaacgttat cgccgacgcc acggccgcct
     1741 ggatgggccg cgccgtgtga atttcggaga gtcctaattc gtcgcgggcg tgtgccccca
     1801 gcgcatcgtg ctccatcagc tgttcggcga cctgctgggc cagcgcgttg tccaggccgc
     1861 gcgatacgta aatcgcggcg agttcgcggc gttcgaatgc ctcgttgtca acgagctcgc
     1921 ggcgctcgcg ttccaggtcc gctttctcgg tgtccgactg cgaactgacc gagacgtatt
     1981 ctccggctgc catcgacatg gcgccggcga cgagcccggc aatgccggcc agcaacacgt
     2041 cgccctgcgc agcccctgcc gccgcgacgc ccacgagcag gctcgccgtc gagacaatgc
     2101 cgtcgttggc gccgagcacg gcggcccgca gccagccgat gcgctccgcg tagtgtcgtt
     2161 cgtgatggcg caccatgtgt gacccttgat tgaccagccc tgccgccatc ccgcccggga
     2221 tgcggagagt cgacgatagc cgattgccgg cgcggcggac ataagcgaat gccgagagtc
     2281 ccgcccggcg ctctcggaga gcggccggct gggccacggc gtgggtgaaa cgcgatgcag
     2341 ccgccgtttt cgatccatgg ggcgccaatc ccacggcaac ggttgcgagt ctcagcgaac
     2401 tcagtggcgt tttctagacg accggtgcct ctcggccgat cacgacggtc ccatttttac
     2461 gcgcattccg cgccgaggga acgcgcaccc ggcccgcgag ttcctgccgg gtgccgctct
     2521 acttcggacc gatcagccgg aggttttccc caggactacc gcgatcacgg ctcccgcgat
     2581 gccgccgcga acgagttcca ggaccgggtc ggtcacggcg agggtcagat tgctgacgtt
     2641 ctgatagccg aaaagaatga agtcgaccgt aaaccagagc aggaaaccga tggttgcgcc
     2701 gaccttagcc ccgtccgccg ccgatacggc cccgctcctc gatcctatcc ccagggtcag
     2761 cagcacggcg tacgacagcg tgccgagcac ggtcgcccaa atcagcggct cctcccgggc
     2821 aacgcccgtc gccgaaccgg cgttctgggc atagaaatcc gcaaagaccg tatcgaatag
     2881 gatgtagccg agtatgtata gcgcgacgag cccgacgagg ctgccgatga ccaggttctt
     2941 gccgttcata atgtcttcct cctgcactga ctgtcactgc agcatcaagt catccaatgc
     3001 tgcacccacc aacacatcga ggaccgttcg actcccgctc gattattcga caacgaaaaa
     3061 atgatccccg gcgccggctc tcgccaccag gcggaatcgg cttatttctt ggttatgggt
     3121 aaagtctagt agaacatttt gagatttccg cggcgcccgc gaactttttt ggcgtcggaa
     3181 gtgggcaacc tgcacgccgt tcaaacggag gcgcgcggct gccgagtttc tttac
//
LOCUS       DATDAA010000457        25755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_208838,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000457 DATDAA010000000
VERSION     DATDAA010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 25755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25755
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208838"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VIS73_13815"
     CDS             <1..394
                     /locus_tag="VIS73_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY5764281.1"
                     /translation="APVWYFTPFYSLLRAVTYPLFGIDAKFWGVVAMGAGVVIFAFLP
                     WLDRSPVKSIRYRGPIFKWLLAVWVVDFIILGFLGTKDPAYMFFGVIPGAPIAQLGSV
                     YYFLFFLAMPLWTKWDKYKTEPERVTFK"
     gene            391..1125
                     /locus_tag="VIS73_13820"
     CDS             391..1125
                     /locus_tag="VIS73_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEY5764282.1"
                     /translation="MKKLLATLLFVPALSWAAGASLHLDRAPDVSGDNAALQNGAKLF
                     VTYCLNCHGASHLRYQKLLDLGFTEEQVKESLMTTADKIGEQMRVAIRPDEAKAWFGA
                     APPDLTLVARARASGDGSGADWLYTYLRSFYKDETRPTGWNNLVFENVGMPHALWELQ
                     GTQVLKTEVGSDGKAHKSLELAKAGLMSAKDYDKQVADLVGFMVWMGEPTAGLRKSLG
                     VGVLIFLFVLFGLAYALKREFWKDIH"
     gene            1209..1805
                     /locus_tag="VIS73_13825"
     CDS             1209..1805
                     /locus_tag="VIS73_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5764283.1"
                     /translation="MMNLYSGTTDPFSHRCRIVLYEKGMDFQIIDVDMFNKPEDLAVI
                     NPHNRVPVLVERDLILYEPNIINEYIDERFPHPQLMPPDPIMRAKARQLLLGLEREMF
                     AHIETLERNLKTADKARQIVRDRLTELAPVVAKQKFLLSDEFSMLDVAIAPLLWRLEL
                     YGIDLPKSSAPILKYAERIFTRQGFIDALTPSEKVMRK"
     gene            1836..2291
                     /locus_tag="VIS73_13830"
     CDS             1836..2291
                     /locus_tag="VIS73_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEY5764284.1"
                     /translation="MEVPSTRPYLIRALHEWCVDNGYTPYISVAVNGETRVPSSFVKD
                     DQITLNISGEATQHLAIDNEAINFAARFGGKPMNIHVPIEQVLAIYARENGVGMAFPV
                     ADGAEEREAAASADAEVNERDIGDSDQEGADAPAPEAPSRGRAKLKRIK"
     gene            complement(2395..2676)
                     /locus_tag="VIS73_13835"
     CDS             complement(2395..2676)
                     /locus_tag="VIS73_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764285.1"
                     /translation="MLSLDECMDFCGLDSEEVEGIAAHEHLPLIVAAELGYQMLQTTE
                     GIAALQAMLLDNIEIARCHGESSRADEYAKIYRHFVAAHPDSPAESRAH"
     gene            3021..5582
                     /gene="gyrA"
                     /locus_tag="VIS73_13840"
     CDS             3021..5582
                     /gene="gyrA"
                     /locus_tag="VIS73_13840"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY5764286.1"
                     /translation="MEQFAKETLPISLEDEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMHELGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQDFSLRYML
                     VDGQGNFGSVDGDNAAAMRYTEVRLARIAHELLGDIEKETVDFGPNYDGSEKEPLVLP
                     AKIPNLLINGSAGIAVGMATNIPPHNLGEVLDGCMALLSNPALSIEDLMTYIPAPDFP
                     TAGFIYGVSGVRDGYMTGRGRVVMRARTHFEDFERGGARQAIIVDELPYQVNKKTLLE
                     RIAELVNDKKIEGISDIRDESDKSGMRVVVELKRGEMPEVVLNNLYKLTQLQDTFGMN
                     MVALVDGQPRLLNLKDLLENFLSHRREVVTRRSVFELRKARERAHVQEGLAVALDNVD
                     EIIALIKAAPTPADARRGLLSRLWPSATVAEMLARAPLDATKPEGVGPEFGLTKKGYL
                     LSEAQAQAILELRLQRLTGLERDKIVTEFGELLEKIADLLDILARAERITTIIGEELQ
                     QIRAQFVDAAKDKRRSEIVLHTQDLGIEDLITPQDMVVTLSHTGYVKAQPLADYRAQR
                     RGGRGKQATGVKEDDFVEHMFVANTHDYILCFSNRGRVYWLKVYEVPQGGRGSRGKPI
                     VNMFPLTEGEKINAVMPVKEFTEDQFVFMATAAGTVKKTPLSEFSRPRPSGIIAVALD
                     DDDYLIGASITTGESDIVLVSNGGKAVWFDEEDVRPMGRPARGVRGMKLGKGQRVISL
                     LVAESEEQTVLVATENGYGKRTVLSEYRHSGRGTQGVIAIQCSARNGKVIGARLVDDD
                     DEVMLITTGGVLIRTRVKEIRGMGRATQGVTLINLGDGELLSGLEKIVESDDDEE"
     gene            5587..6696
                     /gene="serC"
                     /locus_tag="VIS73_13845"
     CDS             5587..6696
                     /gene="serC"
                     /locus_tag="VIS73_13845"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEY5764287.1"
                     /translation="MTRPWNFSAGPAVLPEAVLRQVQEELLDWHGCGASVMEMSHRGR
                     EFGEILSTAEHELRALAGIPPSYRILFLQGGATLQFSQVPLNLLRPDGNGRSADYIVT
                     GAWSRKAYREAARLRKVRCAASTEADGEPSDGFVRLPEPGQAVRNDQAAYLHVCTNET
                     IHGVEIHDDGYFGGAVPVVADMSSHILSRPFDVTRYGLIYAGAQKNIGPAGVTVVIIR
                     DDLIGELRDDLPTALDYRIAADNGSMFNTPPTFAIYVAGLVFGWLRDQGGLATIAARN
                     AEKAELLYGAIDQSALYRNPVESACRSRMNVPFTLQQEGLDDAFLSGARERGLLGLKG
                     HKSVGGMRASLYNAMPIEGVRALVDFLADFERRHA"
     gene            6693..7793
                     /gene="pheA"
                     /locus_tag="VIS73_13850"
     CDS             6693..7793
                     /gene="pheA"
                     /locus_tag="VIS73_13850"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY5764288.1"
                     /translation="MSKTPDDLQSELAPIRAEIDAIDAELLALLNRRAGCAQAVGHLK
                     ARHGQADVVYRPEREAQILRRIQERNPGPLSQESVTWFFREVMSACLSLEQPLGIAFL
                     GPLGTFSESAATKHFGHAARLMPQTTIDDVFREVEAGHADFAVVPIENSTEGAIGRTL
                     DLLLTTPLKICGEVVLRIHQHLLTQEKSLTDIKRVYSHAQSLAQCHEWLNRSLPAAAR
                     ISVASNAEAARLASVEGGVAAIAGAAAADRYELPRLVENIEDEPNNTTRFVVLGRRDA
                     GESGRDKTSLIMSAPNRTGSLHYLLEPFAEAGVSMTRLESRPARQTLWEYVFFVDIEG
                     HRDTAALATALAELGRRAPYVKVLGSYPVAVY"
     gene            7831..8943
                     /gene="hisC"
                     /locus_tag="VIS73_13855"
     CDS             7831..8943
                     /gene="hisC"
                     /locus_tag="VIS73_13855"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY5764289.1"
                     /translation="MSLVDQALPYVQAIMPYQPGKPISELARETGIAEGAILKLASNE
                     NPLGMSPRAAAAMQAALADLARYPDGNGFALKDKLARRLAVAPGQLVLGNGSNDVLEL
                     IARVFLAPGRAAVFAQHAFAVYPLATMATGARGIEVPALDFGHDLPAMQAAITPDTRL
                     VFIANPNNPTGTFVEAEAVRQFIAAVPDDVVVVLDEAYNEYLPPAQRVDSVRWIASHP
                     NLVVTRTFSKIYGLAGLRVGYAVASPAVADLMNRVRQPFNVNSLALAAACAAVDDEEF
                     VSASYEMNRRGMAQIVEGLRTLALRFIPSRGNFITFEVAAGRGGAATVNQRLLALGVI
                     VRPIAGYGLPDWLRVTIGQPEENARFLTALGSALDG"
     gene            8936..9811
                     /locus_tag="VIS73_13860"
     CDS             8936..9811
                     /locus_tag="VIS73_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEY5764290.1"
                     /translation="MAELKKVVVFGVGLIGGSFAAALKAAGSTAEIVGFGRTIGSLQR
                     AQQLGLIDSIGVSLGAEVAAADLILLAMPVGQMAEAMARIAPYLQPGCVVTDAGSTKC
                     DVVAAARAAFGARIGQFVPAHPIAGAENSGPAAARADLFRGRKVVVTPLAENGPEAVE
                     RVRQAWRRCGANLFRLTPEEHDRVFAAVSHLPHLLSFALVNDLAIRDNREVYFEFAAS
                     GFRDFTRIAASDPEMWRDIFFANRDALLDEIDRYAAQLAAFRRALTDDDAQALETMLE
                     LARCARRDWGEKGER"
     gene            9835..11805
                     /locus_tag="VIS73_13865"
     CDS             9835..11805
                     /locus_tag="VIS73_13865"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HEY5764291.1"
                     /translation="METLDLPGMLAARGAVRLPGSKSISNRVLLLAALAEGVTEIREL
                     LESDDTARMLDALRLLGVRIDTATDGALRIQGCAGRFPVRNGDIFLGNAGTAFRPLTA
                     ALSLSPDEAGRSGGSYRLFGVARMHERPIGDLVEGLRQIGCAIDYLGNSGFPPLQIQP
                     AQLGAVGEVRVRGDVSSQFLTGLLMALPLTGQRVTIVVDGELISKPYIEITLAMMRRF
                     GVEVSREQWQRFSIEAGSAYRSAGTVYVEGDASSASYFLAAGAIAGGPVRVEGVGSES
                     VQGDVRFADALAQFGAQLNMGPNWIEARAPAAGGLRAASLDCNHIPDAAMTLAVLALF
                     ADGTTRLNNIGSWRVKETDRIAAMATELRKIGAAVAEGDDWLEVTPPAAFRSAVIDTY
                     DDHRMAMCLSLATLAGVPLRINDPRCVNKTFPDYFSAFAAVTTPVPVIAIDGPSASGK
                     GTVAARVAEALGFAYLDSGALYRVTALAARRQGVDWADEAGVAAIAVGLKVRFESDCV
                     FLEGEDVSAAIRDEQMSSGASQVAALPAVREALLFRQRAFRQAPGVVADGRDMGTVVF
                     ADAGLKVFLTASVETRAERRYKQLIAKGNAVTLAPLLEDLRARDARDSERTVAPLQQG
                     AEARLLDTTALTIDQAVEQVLRWWHAQAPREE"
     gene            12039..13736
                     /gene="rpsA"
                     /locus_tag="VIS73_13870"
     CDS             12039..13736
                     /gene="rpsA"
                     /locus_tag="VIS73_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY5764292.1"
                     /translation="MSAIPATESFAELFEESLARQEMRLGEVITAEVVRIDQNWVVVN
                     AGLKSESYVDVEEFRNDQGELEVAEGDFVQVAIEQLENGYGETRLSRDRAKRVAAWNN
                     LEEALNAGTLVSGTITGKVKGGLTVMTNGVRAFLPGSLVDMRPVKDTTPYEGKTLEFK
                     VIKLDRRRNNVVVSRRAVLEETMGEEREKLLENLKEGAVVKGIVKNITDYGAFVDLGG
                     IDGLLHITDLAWRRVRHPTEVVAVGDEVTAKILKFDQEKNRVSLGMKQLGDDPWVGIS
                     RRYPQSTRLFGKVTNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVNPGKVVQLGDEVE
                     VMILEIDEDRRRISLGMKQCAANPWDDFAMNHKKGDKVNGAIKSITDFGIFIGLPGGI
                     DGLVHLSDLSWSTPGEEAIRNFKKGDEVEAVVLAIDVEKERISLGIKQMEGDPYTSFI
                     ATHEKNTIVRGTVKSIDAKGAVVQLDGDVEGYLRASEAARDRVEDLRALFKEGDEVEA
                     MVINVDRRSRQVSLSIKAKDHAEQHEAMQKLATDAGSSATGTTNLGALLKAKMKEQG"
     gene            13751..14050
                     /locus_tag="VIS73_13875"
     CDS             13751..14050
                     /locus_tag="VIS73_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEY5764293.1"
                     /translation="MTKSELIARLAERFPQLVAKDADYAVKMILDAMTDSLARGDRIE
                     IRGFGSFALNYRPPRVGRNPKSGEKVSVPEKYVPHFKAGKELRERVDTVARSNGS"
     gene            14111..14392
                     /locus_tag="VIS73_13880"
     CDS             14111..14392
                     /locus_tag="VIS73_13880"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEY5764294.1"
                     /translation="MRALVWSLRIAIFLVIFAFAVKNTEMAAVRFPFGFETEVPLIVV
                     MLVAFGFGIFAGVLAMIGPLFRSKRQAAKLRKVRAPEHAPHAIQPPADG"
     gene            14411..15577
                     /gene="lapB"
                     /locus_tag="VIS73_13885"
     CDS             14411..15577
                     /gene="lapB"
                     /locus_tag="VIS73_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEY5764295.1"
                     /translation="MDFEFWQLLLIPVFFGLGWMAARIDIKQLVRESRALPESYFKGL
                     NFLLNEQPDKAIEAFLEVVRVDPQTVELHFALGNLFRRRGEIERAIRMHQNLIEREDL
                     PADLRLQALAELGRDYLKAGLLDRAEESFVKLRGTARDVEAKKSLLEIYQAEKEWLKA
                     IGIARELPEYVSQKELSHFYCELASAETMQSRPDSAMQYLESALTAHRKAVRASALLG
                     DLHAQAGRWSQAIEAWQKIEQQNPPYLALVSQRLMNAYEKLGRRDEGLQLLKGYLEQY
                     PSLDLLDTVIKLVSELQGAPAAQALLKDELRRNPTLLGLDKLLESQLGVAPADKRSDL
                     ELIKNLINTHTRRLARYRCETCGFKARQFYWRCPACGGWESYPPRRSEEFDLSP"
     gene            15650..16198
                     /gene="rfbC"
                     /locus_tag="VIS73_13890"
     CDS             15650..16198
                     /gene="rfbC"
                     /locus_tag="VIS73_13890"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEY5764296.1"
                     /translation="MKARQLAIPEVVLIEPRVFGDDRGFFYESYNRAAFREATGVDPD
                     FVQDNHSKSQRGVLRGLHYQLPPRAQGKLVRVVQGEVFDVAVDIRRGSPTFGKWVGES
                     LSAENHRQMWVPPGFAHGFVTVSETAEFLYKTTDVYAPDCERCIAWNDPMIGIDWRLS
                     GEPLLSAKDRQGVSLAAAEVFG"
     gene            16273..17172
                     /gene="cysM"
                     /locus_tag="VIS73_13895"
     CDS             16273..17172
                     /gene="cysM"
                     /locus_tag="VIS73_13895"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HEY5764297.1"
                     /translation="MGLTLEAFVGNTPLVRLVRLPGEAIANRGNVVLAKLEGNNPAGS
                     VKDRPALSMIARAQARGEIKPGDTLVEATSGNTGIALAMAAALRGFRMVLVMPENQSI
                     ERRQAMAAYGAELVLTPKSGGMELARDVAERMVKEGKGVMLDQFANPDNPLAHYEGTG
                     PEIWRDTNGKVTHFVSSMGTTGTIMGVSRFLKEKNPAIEIVGCQPEEGSQIPGIRKWP
                     EAYLPKIFDRSRVDRVENVSQADAEAMTRRLAAEEGIFAGISSGGAISVALRLAAELD
                     NAVIVAIICDRGDRYLSTGIFPG"
     gene            17193..17990
                     /locus_tag="VIS73_13900"
     CDS             17193..17990
                     /locus_tag="VIS73_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY5764298.1"
                     /translation="MIPTLTFDLETIPDVAGLRTLYGLDASSPDALPDAEVAELAFQR
                     RRAQNGTDFLPLHLHRIVTISCALRAGDAFKVWSLAAPELDEGAIIQRFFDGIEKFTP
                     QIVSWNGGGFDLPVLHYRGLIHGVAAPRYWDLGDGDYGDSREFKWNNYISRYHTRHLD
                     LMDLLAMYQPRANAPLDELAKLIGFPGKLGMDGSQVWSAWLNGKAAEIRDYCETDVVN
                     THLVALRFRKMRGELSADGYAAEVDLVRHSLAQLDQPHWREFLEAWP"
     gene            18051..20765
                     /locus_tag="VIS73_13905"
     CDS             18051..20765
                     /locus_tag="VIS73_13905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY5764299.1"
                     /translation="MPEIDPSEKMSDALVLCATHRLARRLSLAGEGAIRRLRPALTVG
                     QWLDARTEAALLGGEMAAPPRRLSRLQERLLWRRVIDEALAGDAIAALLDRDGLVEAA
                     IEANALIETWGVAAGGGHNEDARQFLAWRQAFRRRCDAGGWRDAARYQGWQIGSVVAG
                     AGLPKAVMFAGFDRFNPQEQRLLDALSGAGVAVRVEQPDPATPASHCLAFDSPDRELA
                     AAIAWAQGVFVAQPAARVGIVVPELAKRRATLLRVLDETFAPATMRAAAADAPRPYNL
                     SLGESLNEAPGIATALELLRLALPGARPTQQELRTVLDSPWWGGGVAERLGRGQLDAD
                     LREHLPPEVDGATLLRRTWRLHYRDGGLERTARSLADLAAESARWRGRQPPSAWATAF
                     AAALDAAGWPGERAASSREFQAQEAFAETLNALAALDEFAGRVGAGEAVTLLAEHCRE
                     MIFQPRTEGAPRLQVLGLLEAATESFDALWVCGMSDAVWPPPARPNPFLHPAAQRAAG
                     SPNASSEVQFRFATAIHARLLRAAPAVRFSWAIQDEARVQLPSALLGADPAAALAAAA
                     ETAGASELAARLRAQAGDAAIEQVADRYGPPVAAGETVRGGTHLIRTQAACPAWGYYQ
                     YRVGARALKTPVEGLDSAGRGTIVHAVFEAFWRDRDSAGVFGWDEAVLAMRIGEAVTA
                     GIAAFEDERGEPLPPRFRALETTRLAALLAEWLAVERQRGVPFVVAGCEVERQLSLRG
                     LPIRVVADRVDRLADGRLVIIDYKTGRAPALSAWATQRIVEPQLPIYAAGESGEPVAA
                     VAFADLRARPPKFAGLAAENGLLPGVGDVDGARRHFDADSFPDWQAVLATWQARLLML
                     ADEVHAGDAAVRVDNPKTLAYAEVLPLLRLAERDWLFGAAGADGGDDA"
     gene            20758..24174
                     /locus_tag="VIS73_13910"
     CDS             20758..24174
                     /locus_tag="VIS73_13910"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY5764300.1"
                     /translation="MPESMCAGMPTLADDAAARERALEPGSFIVEAPAGAGKTELLTQ
                     RFLALLARVESPEEVVAITFTRKAAAEMRGRIVDNLIAAASAPRPDAPHRARTWELAR
                     QALAHGAARGWGLERQPARLRVLTIDALCMLLARQAPLLSQIGGIASVSDDSDALYAE
                     AARRSLALLEGDDTAHADCVAAALAHLDNDAGRLQRLLAAMLARRDQWLPHGRPVEAG
                     VGNRAALAVIVEAALADVASTVDGGAQAALMPLFAEALANAPEATAAQLAPLAEWQPL
                     LVAAWDELPRWQALARWLLTQKGEFRKRWTANEGFPAGKAGQGAKAAIGEWIAALPVS
                     AEAGLAAVQALPTPEDAAADLPLVEALARLLWLAVAQLWLVFGETREVDFIAVAEAAL
                     RALGERDAPSDLALDYRITHLLVDEFQDTSPSQVELLQRLTAGWGEAEGDERTLFCVG
                     DPMQSIYRFRKAEVGLFIEARRRGIGSIRLAPLQLARNNRSDEGLVDWINATLPAAFA
                     SADDPLRGAIAYRRFVATREGRPGSGAFWHPLVCERGVPASAVATAEAGRIIALVDEL
                     RASDGEREIAVLVRARSHLTALVAQLRRNRPDLPFEAVEIDSLESRQWVRDLIILASA
                     LGQLADRVNWLALLRSPLCGLTLVDLHALAAGTGRTSPWALLHDDARLATLSADGRER
                     ALALRVVIGEALAHAGRQRRARWVEGVWLALGGAATVPDEAALADCRACFDLIEHLSA
                     AGRFDLDTLPAEVGKLFAAADPRGAGRLTLMTIHKAKGLEFDTVILPGLQRAPGGFDD
                     PPLLRWESVIAEDGTRHTAVAALPPREGAGGEASYRLLERLERDRGRAEAARVLYVGA
                     TRAKRALHLFASVWQSADGEALAPPRGESLLGFLWPAVAEEWAALPVDLAADAAAAGA
                     AQLADFVPPLRRLSSAGFAARLSATLPGDVDAGSGCDDSAAARDQAVMHRIDWGRGAN
                     AREGLIGTLTHRYLERIAVDGLAAWPAERFDGLHGAMRDWLVREGLPPIEAAASAAEV
                     AAMLRLTRQSADGQRVLRAKGDAELALVSEDADGARVQVVDRSFIDGGVRWVVDYKTE
                     AVGDGNFTAAAARHREQLARYAKLFAGESRPIRCAVFFVRYGRLESLSDE"
     gene            24310..25560
                     /locus_tag="VIS73_13915"
     CDS             24310..25560
                     /locus_tag="VIS73_13915"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY5764301.1"
                     /translation="MKRVDDYRLKFGKHELVPIMVGGMGVDISTAELSLEAARLGGVG
                     HISDAMVPTVSDRRFKTKYVKNKLKQYKFNVENADKSVVQFDLGLLAEATAMHVGRTM
                     EAKRGPGLIFINCMEKLTMNAPKETLRVRMSSALDAGIDGITLAAGLHLGSFALIEDH
                     PRFRDVKLGIIVSSLRALQLFLKKSARTGRLPDYVVIEGPLAGGHLGFGMDWAQYDLA
                     KIVIEIRDWLAAEKLDIPLIPAGGIFTGTDAVNFMEMGAAAVQVATRFTVSAECGLPE
                     DVQQEYFKASEEDIEVNTISPTGYPMRMLKSSPAIGDGIRPNCEAYGYLLDAKGNCQY
                     IEAYNREVAAHPEAKKVKVFDKTCLCTHMRNFDCWTCGHYTYRLKDTTVRDAEGKYQI
                     LSAEHIFKDYQFSTDGKIALPPAA"
BASE COUNT         4438 a         8363 c         8461 g         4493 t
ORIGIN      
        1 cgcgccggtt tggtatttca cgccgttcta ctcgttgttg cgtgcggtca cctatccgct
       61 cttcggcatt gacgcaaaat tctggggtgt cgtcgcgatg ggcgccggtg tggtgatctt
      121 cgccttcctg ccctggctag accggtcgcc ggtcaagtcg atccgttacc gcgggccgat
      181 ctttaagtgg ctgctcgccg tctgggtggt ggatttcatc atccttggat tcctcggtac
      241 caaggacccc gcctatatgt tcttcggcgt gatcccgggt gcgccgattg cgcagctcgg
      301 cagcgtctat tacttcctgt tcttcctggc gatgccgttg tggacgaagt gggacaaata
      361 caagactgaa ccggaaaggg tgacgttcaa atgaaaaagc ttctcgccac tttgctcttc
      421 gtgccggcgc tttcctgggc cgccggtgca tcgttgcatc tggatcgggc gcccgacgtg
      481 tcaggcgaca acgccgcgct gcagaacggc gcgaagctgt tcgttaccta ttgcctaaac
      541 tgccacggcg ccagccatct ccgttaccag aaactgctcg atctcggttt taccgaggag
      601 caggtcaagg aaagcctgat gacgaccgcc gacaagatcg gcgaacagat gcgggttgcg
      661 attcgtccgg acgaggcgaa ggcttggttt ggcgccgcgc cccccgacct gacgcttgtt
      721 gcgcgcgcac gcgcctcggg tgacggttcg ggcgccgact ggctatatac ctacctgcgc
      781 tcgttctaca aggacgagac tcggcccact ggctggaaca acctggtctt cgagaacgtc
      841 ggcatgccgc acgccctctg ggaattgcag ggaacgcagg tgctaaagac cgaggtcggg
      901 tcagatggca aggcgcacaa gtcgctggag ctggcaaagg caggcttaat gtctgcaaag
      961 gactacgaca agcaggttgc cgatctggtt ggtttcatgg tctggatggg cgagccgacc
     1021 gccggcctgc gcaagtccct cggcgttggc gtgctgatct tcctgttcgt gctattcggc
     1081 ctcgcttatg cgctgaagcg cgaattctgg aaagacatcc attaatcagc gttgaagtca
     1141 ttttcgccgg tacagcgggg cggccaatcc gtctgctgta ccggttagtc ttttaggaat
     1201 cgtcaatcat gatgaacttg tactccggaa ccaccgatcc attcagccat cgttgccgaa
     1261 tcgtcctcta cgaaaagggg atggatttcc agatcattga cgttgacatg ttcaacaagc
     1321 ccgaggacct ggcggtgatc aatccgcaca atcgggtccc ggtactcgtc gagcgcgacc
     1381 ttattctcta cgagccgaac atcatcaacg agtacatcga cgagcgtttc ccgcatccgc
     1441 agctgatgcc gcccgacccg atcatgcgcg ccaaggcccg gcagctcctc ctaggcctcg
     1501 agcgcgagat gtttgcacac atcgagacgc tggagcgaaa tctgaagact gctgacaagg
     1561 cacgccagat cgtccgtgat cgtctgaccg aactggcgcc ggtcgtcgcc aagcagaagt
     1621 ttctgcttag cgacgagttc tcgatgctcg atgtggcgat cgcgcccttg ctctggcggc
     1681 ttgaactcta cggaatcgac ctgccgaaat catccgcgcc cattctcaag tacgcggaac
     1741 ggatcttcac gcgtcagggc ttcatcgacg cgctgacgcc gtcggaaaag gtgatgcgca
     1801 agtgatcgct gcgcgtgcgg cgaaggccag ttgagatgga agtcccatcg accaggccgt
     1861 atttgatccg cgcgctgcac gagtggtgtg ttgacaacgg ctacacaccc tacatttcgg
     1921 tggccgtgaa cggagagact cgcgttcctt catccttcgt caaggacgac cagatcacgc
     1981 tcaacatcag cggcgaggca acgcagcatt tggccatcga caacgaggcg atcaacttcg
     2041 cggcccgctt cggtggcaag ccgatgaaca ttcatgtgcc gatcgagcag gttctggcga
     2101 tctatgcccg cgaaaatggt gtcggcatgg cgtttcccgt cgccgacggc gctgaagaac
     2161 gcgaagcggc cgcctcggcc gatgcagagg tcaacgagcg cgatatcggg gactctgatc
     2221 aggagggcgc cgacgctcca gcaccggagg cgccgagtcg cggtcgcgca aagctgaagc
     2281 ggatcaagta aggggcgctt gccttacgcg cggtgtctgc gccgcagcac tatttgccga
     2341 cccgcggttc ggcagacatg tcgtctctgg gacggccagg tcgccaattc gactttagtg
     2401 ggcgcgggat tcggcaggtg aatccggatg ggccgcaaca aagtgccgat agatcttcgc
     2461 gtactcgtcg gcacgtgaac tttcgccatg gcagcgggca atctcgatgt tgtcgagcag
     2521 cattgcctgc agtgcggcga ttccctcggt ggtctgcagc atctggtagc caagttcggc
     2581 tgccacaatg agcggcaagt gctcatgcgc agcaattccc tcgacttcct cgctgtcgag
     2641 gccgcaaaaa tccatgcatt cgtccaggct caacatcgct gtctcctgct catgcgccgg
     2701 gtcagcaaaa tggcctcggc tattgacggt cggcgcctcg ttttgagtac cgtccctgac
     2761 ggaaatccaa ccgttcttct gccaccggcg cggctggtca ggtcatcgta gatgctcaga
     2821 atcgcaaatc aagcgcgaag cagaggcgac gctgcccctc ccgtgccacg gccctgttgc
     2881 aagccattga attatggggt gaaaaaaaag gctagccgat tggctcctgg ccactcccgg
     2941 cgggcgtttc ggcaggtcga tatgataaaa tcgacgggtt ttttcgagcg tcggaccgct
     3001 gagaataatc aataaatcct atggaacaat tcgcaaaaga aacattgccg atcagcctcg
     3061 aagacgagat gcggcgcagc tatctcgatt atgcaatgag cgtgatcgtg ggccgcgcgc
     3121 tgcccgatgt gcgggacggt ctcaagccgg tccaccggcg cgtgcttttt gccatgcacg
     3181 aacttgggaa cgactggaac aaggcctaca agaaatcggc tcgcgtcgtc ggcgacgtca
     3241 tcggtaaata ccacccgcac ggcgattcgg ccgtttacga cacgattgtc cgcatggcgc
     3301 aggatttttc gctgcgctac atgctggtgg acggacaggg taacttcgga tcggtcgatg
     3361 gcgacaacgc ggcggcgatg cgctacaccg aagtccgtct ggcgcgaatc gcccatgagc
     3421 tcctgggcga catcgaaaag gaaaccgttg atttcgggcc aaactacgac ggttcggaga
     3481 aggaaccgct ggtcctgccg gcgaagatcc ccaacctgtt gatcaacggc tcggccggca
     3541 tcgcggttgg catggcaacg aacattccgc cgcacaatct cggcgaggtg ctcgatggct
     3601 gcatggcgct gttgagcaat ccggcgctgt cgatcgaaga tctaatgact tacatcccgg
     3661 cgccggattt tccgaccgct ggatttattt atggcgtttc gggcgtgcgc gacggctaca
     3721 tgaccggccg cggccgcgtt gtgatgcgcg cgcggacgca cttcgaggat ttcgagcggg
     3781 gcggcgcgcg gcaggcgatt atcgtcgacg agctgccata ccaagtcaac aagaagacgc
     3841 tactcgaacg catcgccgag ctggtgaacg acaagaagat agagggcatc agcgatattc
     3901 gcgacgaatc ggacaagtcc ggcatgcgcg ttgtcgtcga gctcaagcgt ggcgagatgc
     3961 ccgaggtcgt tctcaataac ctttacaagc tgacgcagct gcaggatacc ttcggcatga
     4021 acatggtggc gctggtcgat ggccagccgc gcctgctgaa tctgaaggat ctgctcgaga
     4081 acttcctctc gcaccgccgc gaggtcgtca cccggcgttc ggtgttcgaa ttgcgcaagg
     4141 cacgcgagcg tgcgcacgtc caggaaggcc tggcggttgc gctcgacaac gtcgacgaga
     4201 tcattgccct gatcaaggcg gcgccgacgc cggccgatgc acgacgcggc ctgctctcgc
     4261 gcctgtggcc ttcggcaacg gtcgccgaga tgctggcgcg ggcgccgctg gacgcgacta
     4321 agcccgaggg ggtcggaccg gaatttgggc tgaccaagaa gggttatctg ctctcggaag
     4381 cgcaggcaca ggcgatcctc gaactccggc tgcagcgcct gactggcctc gagcgcgaca
     4441 agatcgttac cgaattcggt gagctgctgg aaaagatcgc cgacctgctc gatatcctgg
     4501 cgcgggccga gcggatcacg acgatcatcg gcgaggagct gcagcagatt cgggcgcagt
     4561 tcgtcgacgc ggccaaggac aagcggcgca gcgagatcgt cctgcacacc caggatctcg
     4621 gtatcgaaga cctcatcacg ccgcaggaca tggtggtcac cctgtcgcat acgggttacg
     4681 tcaaggcgca gccgctcgcc gactaccggg cccagcgccg cggtggacgt ggcaaacagg
     4741 cgaccggcgt caaggaagac gacttcgtcg agcacatgtt cgttgccaat acccacgact
     4801 acattctctg cttctccaat cgcggccgtg tctactggct gaaggtgtac gaagtgccgc
     4861 agggtgggcg tggctcacga ggcaagccga tcgttaacat gttcccgttg accgaaggcg
     4921 agaagatcaa cgccgtcatg cccgtcaagg aattcaccga agatcagttc gtcttcatgg
     4981 cgaccgctgc cggaacggta aagaaaactc cgctgtccga gttttcgcgc ccgcgcccgt
     5041 cggggatcat cgccgtcgca ctcgacgatg acgactacct gatcggcgct tcgatcacca
     5101 ccggcgaaag cgacatcgtc ctggtctcga acggcggcaa ggcggtctgg ttcgacgaag
     5161 aggacgtccg cccgatggga cggccggcgc gcggcgttcg cggcatgaaa ctgggcaagg
     5221 ggcagcgcgt gatttccttg ctcgttgccg agagcgaaga acaaaccgtt ctcgtcgcca
     5281 ccgaaaacgg ctacggaaaa cgcacggtgc tctccgaata ccggcacagc ggccgcggaa
     5341 cgcagggcgt gatcgcgatc cagtgttcgg ctcgcaacgg caaggtaatc ggcgcgcgcc
     5401 tcgtcgacga cgacgatgag gtgatgctga ttaccactgg cggcgtgctg atccgaaccc
     5461 gggtcaagga aatccgcgga atgggtcggg ccacgcaggg cgttaccctg atcaacctgg
     5521 gcgatggcga acttctctcg ggtctggaga agatcgtcga gagtgacgac gacgaggagt
     5581 gagtcggtga cgcgcccgtg gaatttctcg gcggggccgg cggtattgcc ggaggcggtg
     5641 ttgcgccagg ttcaggagga actgctcgac tggcacggtt gtggcgcctc ggtcatggag
     5701 atgagccacc gaggccgtga attcggcgaa atcctttcta ctgccgaaca cgaactccgc
     5761 gcgcttgccg gcatcccgcc gtcgtaccgc atcctgttcc tgcagggtgg ggcgacgctg
     5821 cagttctcgc aggttccgct gaacctgctg cgcccggacg gcaacggccg cagcgctgac
     5881 tacatcgtca ccggtgcctg gtcgcgcaag gcttatcgcg aggcggcacg cctgcgcaag
     5941 gtccgttgtg cggcgagtac cgaggcggac ggcgagcctt cggacggttt cgtccggctg
     6001 cccgagcccg gacaggccgt gcgcaacgac caggcggcct acctgcacgt ctgcaccaac
     6061 gaaacgattc acggcgtcga gatccatgac gacggctatt tcgggggtgc ggttccggtg
     6121 gtcgccgaca tgagctcgca catcctgtcg cgccccttcg atgtcacccg ctacggcttg
     6181 atttacgctg gcgcccagaa aaatatcggt ccggccggcg tcacggtcgt gatcatccgc
     6241 gatgatttga tcggcgagct gcgcgatgac ttgccgacgg cgctcgacta tcggatcgct
     6301 gccgacaacg gctcgatgtt taacaccccg ccaaccttcg caatctacgt cgccggtctg
     6361 gttttcggct ggctgcggga ccagggaggg ctcgccacga ttgcggcgcg caatgcggaa
     6421 aaggccgaac tgctctacgg cgcgattgat cagagcgcgc tctaccgaaa tccggttgaa
     6481 tcggcctgcc gctcgcgaat gaatgttccg tttacgctgc agcaggaagg gctcgacgac
     6541 gctttccttt ccggggccag ggagcgtggc ttgctcgggc tcaagggcca caagtcggtc
     6601 ggtggcatgc gtgcctcgct ctacaatgcg atgccgatcg agggcgtccg cgctctcgtt
     6661 gatttcctcg ctgatttcga acgacgacac gcatgagcaa gacgcccgac gacctccagt
     6721 cagaactggc gccgattcgc gccgagatcg atgcaataga tgccgaactg ctggctctgc
     6781 tcaatcggcg tgccggctgc gcgcaagccg ttggtcacct caaggcacga cacgggcagg
     6841 ccgatgtcgt ctaccgtccc gaacgcgagg cgcagatcct gcgccggatc caggaacgca
     6901 acccggggcc gctaagccag gagagcgtca cctggttctt ccgcgaagtg atgtcggcat
     6961 gcctgtcact cgagcagccg ctcggcatcg ctttcctcgg gccactcggc accttttcgg
     7021 aatcggcggc gaccaagcac ttcggtcacg ccgcgcgcct catgccgcag accacgatcg
     7081 atgacgtctt ccgtgaggtc gaggccgggc acgccgattt cgccgtagtg ccgatcgaga
     7141 attcaaccga aggcgcgatc ggacggaccc tcgatttgct gctgacgacg ccgctgaaaa
     7201 tctgcggtga ggtcgtgctg cgcattcacc agcatctgct gacccaagaa aagtcgttga
     7261 ccgacattaa gcgcgtctac tcgcacgccc agtcgctcgc ccagtgccat gagtggctga
     7321 accgctcgtt gccggcggcg gcgcggatct cggtggcaag caatgccgag gcggcgcgcc
     7381 tggcgtcggt cgaggggggc gtggcggcga tcgccggcgc ggccgccgct gatcgctacg
     7441 aactgccgcg actcgttgag aacatcgagg acgaaccgaa caacaccacg cgtttcgtcg
     7501 tcctcggtcg ccgcgacgcc ggcgagtcgg gccgagacaa gacctcgctg atcatgtcgg
     7561 cgccgaaccg caccggctcg ctgcattacc tgctcgaacc cttcgccgag gccggggtgt
     7621 cgatgacccg gctcgaatca cgaccggcgc ggcagacgct ctgggagtac gtcttcttcg
     7681 tcgacatcga aggtcaccgt gacaccgcgg cgctggccac ggcgctcgcg gagttgggcc
     7741 ggcgcgcacc ctacgtcaag gtccttggtt cctacccggt ggctgtctac tgatcgtgcg
     7801 atcgctgccg aatcgcgctg tggagtgccg atgagcctcg tcgaccaggc cctgccctac
     7861 gtgcaggcga tcatgcccta tcagccgggc aaaccgattt cggagctggc acgcgaaacc
     7921 gggattgccg agggcgccat cctcaagctt gcctccaacg agaatccgct cggcatgagt
     7981 ccacgcgccg ctgcagcgat gcaagctgcg ctcgctgatc tggcgcgcta cccggacgga
     8041 aacggctttg cgctgaaaga caagctggca cggcgtctgg ccgtcgcgcc cggacagctg
     8101 gttctcggca acggctcgaa cgatgtcctc gaactgatcg cgcgggtctt cctcgcgccc
     8161 ggccgtgcgg ccgtttttgc gcagcacgcc tttgccgtct atccgctggc gacgatggcc
     8221 accggtgccc gtggcatcga ggttccggcg cttgatttcg gtcatgacct gccggcgatg
     8281 caggcggcga tcacacccga tacgcgcctg gtcttcatcg ccaatccgaa caatccgact
     8341 ggcacctttg tcgaggctga ggcggttcgc caatttatcg ccgctgttcc agacgacgtc
     8401 gtggtcgtcc tcgacgaggc ctacaacgag tatctgccgc cggcgcaacg cgtcgactcg
     8461 gtgcgctgga tcgccagtca tccgaacctc gtggtgacgc gtaccttctc gaagatctac
     8521 ggactcgccg gcctgcgcgt cggttacgcc gttgcgtcgc ccgctgtcgc cgacctgatg
     8581 aatcgcgttc gccagccctt caacgtcaac agtctggcgc tcgctgccgc ttgcgccgcg
     8641 gttgacgacg aggaattcgt cagcgccagc tacgagatga accggcgcgg catggcgcag
     8701 atcgtcgaag gactgcggac gctagccctg cgcttcattc cgtcccgcgg caacttcatc
     8761 acctttgaag ttgctgcggg acgcggcggc gcggcgaccg tcaaccaacg cctgctggcg
     8821 ctcggtgtca tcgtccggcc gatcgccggc tacggtcttc ccgactggct gcgggtgacg
     8881 atcggacagc ccgaggagaa cgcgcgcttc ctcacggcgc tcggcagcgc gctcgatggc
     8941 tgagctgaaa aaggtcgtcg tctttggcgt cggcctgatc ggcggctcat ttgcggcggc
     9001 cctcaaggcg gccggcagca cggccgaaat cgtcggcttc ggccgcacga tcggcagcct
     9061 gcagcgcgcg caacaactcg gcctgatcga cagtatcggc gtcagtctcg gcgccgaagt
     9121 cgcggcggca gacctgatcc tgctggcgat gccggtcggc cagatggccg aggcaatggc
     9181 acgcatcgcg ccctacttgc agccgggctg cgtggtcaca gacgccggta gcaccaaatg
     9241 cgatgtcgtg gccgcagcgc gcgccgcctt tggtgcgcgg atcggccagt tcgtgccggc
     9301 gcatccaatt gccggtgccg agaacagcgg cccggccgcg gcgcgtgccg accttttccg
     9361 cggcagaaaa gtcgtggtga caccgctcgc cgaaaacggc ccggaagccg tcgagcgcgt
     9421 gcgtcaggcc tggcgccgct gcggcgccaa cctgttccgc ctgacacccg aggaacacga
     9481 tcgcgtcttt gctgccgtct cgcatctgcc tcacctgctg tcgttcgcac tggtcaacga
     9541 tctggcgatt cgcgacaacc gcgaggtcta tttcgaattc gccgcctcgg gctttcgcga
     9601 tttcacgcgg atcgccgcga gtgaccccga aatgtggcgc gacatcttct ttgccaaccg
     9661 cgacgcgctg ctcgatgaaa tcgaccgtta cgcggcccag ctcgcggcct tccgccgcgc
     9721 actcacggac gatgatgcgc aggcgctgga gacgatgctc gaactcgcgc gctgcgctcg
     9781 ccgtgactgg ggcgagaagg gcgaacgatg agcaatcgcg ccaaggacga gcaaatggag
     9841 accctcgatc tgccgggcat gctggcggcg cgtggcgcgg tccgcctgcc gggatcgaag
     9901 agcatttcga atcgtgtcct gctgctcgcg gcgctggccg agggtgtcac cgaaatccgt
     9961 gagctgcttg aatccgacga tacggcccga atgctcgatg cgctgcgtct gcttggcgtg
    10021 cgaatcgaca ccgccaccga tggcgcgctg cgaattcaag gctgcgctgg ccgctttccg
    10081 gtccgcaacg gcgacatctt cctcggcaat gccggcactg cgttcaggcc actgacggct
    10141 gcgctgtcgc tgtcgcccga cgaagcgggc cgatcgggtg gcagctaccg tctgtttggc
    10201 gtggcacgca tgcacgagcg tccgatcggc gatctggtcg agggcttgcg ccagatcggc
    10261 tgcgcgatcg actaccttgg caattcgggg ttcccgccgc tgcagatcca gcccgcgcag
    10321 ctcggtgccg tcggcgaggt gcgcgttcgc ggcgacgtct cgagccagtt cctcaccgga
    10381 ctgctgatgg cccttccgct gaccggccag cgcgtgacca tcgtcgtcga cggcgaactg
    10441 atttccaagc cctacattga gattacgctg gcgatgatgc gccgcttcgg tgtcgaggtc
    10501 agtcgcgagc agtggcagcg gttttcgatc gaggccggat ctgcctaccg cagtgccgga
    10561 accgtgtacg tcgagggcga cgcctcgtcg gcatcgtact ttctggcggc cggcgcgatc
    10621 gccggcggcc cggtccgagt tgagggtgtc ggcagcgaaa gcgttcaggg cgacgttcgc
    10681 tttgccgatg cactggcgca attcggtgcg cagctcaata tggggccgaa ctggatcgag
    10741 gcgcgggcac cggctgccgg tggcctgcgc gcggcaagcc tcgattgcaa ccacattccc
    10801 gatgcggcga tgacgctggc cgttctcgcg ctcttcgccg atgggacgac gcggctgaac
    10861 aacatcggca gctggcgggt caaggagacc gaccgcatcg ccgcgatggc gaccgaattg
    10921 cgcaaaatcg gcgcggccgt tgccgagggc gacgactggc ttgaagtgac gccgcccgcg
    10981 gcgttccgtt cggccgtaat cgatacctac gacgatcacc ggatggccat gtgcctgtcg
    11041 ctggcaacgc tggccggcgt gccgctgcga atcaacgatc cgcgctgcgt gaacaagacc
    11101 ttccccgact acttttcggc tttcgccgcg gtaacgaccc cggtgccggt catcgccatc
    11161 gacgggccgt cggcatcggg caagggtact gtcgctgccc gtgtcgccga ggcgctcggt
    11221 ttcgcctatc ttgactcggg cgcgctctac cgggttaccg cgctcgcggc tcgccggcaa
    11281 ggcgtcgact gggccgatga ggctggtgtc gcagccatcg ccgtcggctt gaaggtgcgc
    11341 ttcgaatccg actgcgtgtt cctcgaaggc gaggacgtgt cggcggcgat tcgcgacgag
    11401 cagatgtcgt cgggcgcgtc gcaggtcgcg gcgctgccgg cggttcgcga ggcgctgctg
    11461 ttccgccagc gcgccttccg gcaggcgccc ggcgtcgtcg ccgacgggcg cgatatgggc
    11521 acggtcgtct ttgccgacgc gggcctcaag gtctttctca ccgcgagcgt cgaaacacgc
    11581 gctgagcggc ggtataaaca gttgatcgca aagggaaacg ctgttacact cgcccccctc
    11641 ctggaggacc tgcgggcgcg tgacgcgcgc gacagcgagc gtacggtggc tcccctgcaa
    11701 caaggtgccg aagcgcggct gctcgacacg accgcgctga cgatcgacca ggccgttgag
    11761 caggtcctgc gctggtggca tgcgcaagct ccacgggaag agtgattcgt gccgcatcgg
    11821 cctttgctgg ccgcggcggc agacgcattt ctgctgatgc agtggtaaca gggccggttt
    11881 ccggcttgta gcaaaggccg gttcgccggt cgtggatgag cttcttcgat gttgcgtcgg
    11941 aaggcgcagc gggtgttcga cgccggccgg aaccggacga cgcccggaga agcatttttt
    12001 caactaaccc gccgcatccc gcggcaatga aggtctacat gtctgccatc cccgcaaccg
    12061 aaagttttgc cgaactgttt gaagaatctc ttgcacgcca ggagatgcgc ctcggtgaag
    12121 tgatcaccgc cgaagtcgtt cgtatcgatc agaactgggt cgtcgtcaac gccggactca
    12181 agtccgagag ctatgtcgac gtcgaagaat tccgcaacga ccagggagag ctcgaagttg
    12241 ccgaaggcga cttcgtgcag gtcgccatcg agcagctcga gaacggctac ggcgagacgc
    12301 gtctgtcgcg agaccgtgcc aagcgcgttg ctgcctggaa caacctggaa gaggcactta
    12361 atgccggtac gctggtcagc ggcaccatca ccggcaaggt caagggcggt ctgaccgtca
    12421 tgaccaacgg cgtgcgcgcc ttcctgccgg gttcgctggt cgacatgcgg ccggtcaagg
    12481 acaccacccc gtacgaaggc aagacgctcg agttcaaggt gatcaagctt gatcgccgtc
    12541 gcaacaacgt tgtcgtttcg cgtcgcgccg ttctcgagga gacgatgggc gaggagcgcg
    12601 agaagctgct cgagaacctc aaggaaggtg ccgtcgtcaa gggcatcgtc aagaacatca
    12661 ccgactacgg tgcgttcgtc gacctcggcg gcatcgacgg cctgctgcac atcactgatc
    12721 tggcctggcg tcgcgttcgc catccgaccg aagtggttgc ggttggcgac gaagtcaccg
    12781 ccaagatcct caagttcgac caggaaaaga atcgtgtttc gctgggcatg aagcagcttg
    12841 gcgatgaccc gtgggtcggc atttcgcgcc gctacccgca gagcacgcgt ctcttcggca
    12901 aggtcaccaa tctcaccgac tacggtgcct tcgtagagat cgagcagggc atcgaaggtc
    12961 tggtccacgt ctctgaaatg gactggacca acaagaacgt caatccgggc aaggtcgtcc
    13021 agctcggcga cgaagtcgaa gtaatgatcc tcgagatcga cgaagaccgt cgccgtattt
    13081 cgctgggcat gaagcagtgt gcggcgaacc cctgggacga cttcgcgatg aatcacaaga
    13141 agggcgacaa ggtcaacggc gcgatcaagt cgatcaccga ctttggcatc ttcatcggcc
    13201 tgcccggtgg catcgacggc ctcgtgcatc tctccgacct gtcgtggagc acgccgggcg
    13261 aagaggcgat ccgcaacttc aagaagggcg acgaggtcga ggcagtcgtg ctcgccatcg
    13321 atgtcgagaa ggagcgcatc tcgctgggca tcaagcagat ggaaggcgat ccctacacca
    13381 gcttcatcgc cacgcacgag aagaacacca tcgttcgcgg caccgtgaag tcgatcgacg
    13441 cgaagggagc cgtggttcag ctcgacggtg acgtcgaagg ctacctgcgt gcttcggaag
    13501 ctgcccgcga ccgcgtcgaa gatctgcgtg cgctgttcaa ggaaggcgac gaagtcgagg
    13561 cgatggtcat caacgtcgat cgccgttcgc gtcaggtcag cctgtcgatc aaggccaagg
    13621 atcatgccga gcagcacgag gcgatgcaga aactcgccac cgatgctggt tcctcggcaa
    13681 ccggcacgac aaacctcggc gccctgctga aggccaagat gaaggaacag ggctgatcga
    13741 gcgcgccagt atgaccaagt cggaactcat cgctcgcctc gccgaacgct tcccgcaact
    13801 cgttgcgaag gatgctgact acgcggtgaa gatgattctt gacgcgatga ccgacagtct
    13861 cgcccggggt gaccggatcg agattcgcgg cttcggtagt tttgccttaa actaccgacc
    13921 gccgcgcgtt gggcgcaatc cgaagtcggg cgagaaggtc agcgttccgg agaagtatgt
    13981 gccgcacttc aaggcgggga aggaactccg cgagcgcgtc gataccgtgg cgcgcagcaa
    14041 cggttcctga tccaccggcg agatcggatg acgggcggca ttaagccgcc cgttttttat
    14101 tggagaatcg atgcgcgcac tggtctggag tctgcgaatc gccatatttc tggtgatctt
    14161 cgcattcgca gtcaagaaca ccgagatggc cgccgtccgt tttccgttcg gctttgaaac
    14221 cgaggtgccg ctgatcgtgg tcatgctggt cgccttcggc tttggcatct tcgccggcgt
    14281 actggcgatg atcggtccct tgttccgcag caagcggcag gccgccaaac tgcgcaaggt
    14341 gcgggcgcca gaacacgcgc cgcatgcgat ccagccaccc gcagacggct gatcgcgcct
    14401 gacgatgccg ctggatttcg agttctggca gctcctgctg atcccggtat tcttcgggct
    14461 cgggtggatg gcggcgcgaa tcgacatcaa gcagctggtc cgcgaatcgc gggccttgcc
    14521 cgagtcgtat ttcaagggcc ttaacttcct cctcaacgag cagcccgaca aggccatcga
    14581 ggcctttctc gaggtcgttc gcgtcgaccc ccagaccgtt gagttgcatt ttgcgctggg
    14641 caatctgttt cgccggcgcg gcgagatcga gcgcgcgatc cgcatgcacc agaatctcat
    14701 cgagcgcgaa gatctccccg cggacctccg actgcaggca ctggccgaac tcggccgcga
    14761 ttacctcaag gccggcctgc ttgatcgcgc cgaggagagc ttcgtcaagc tgcgcggcac
    14821 cgcgcgcgat gtcgaagcca agaagagctt gctcgagatc taccaggccg agaaggaatg
    14881 gttgaaggca atcggcatcg cccgcgaact gcccgagtac gtcagccaga aggaactctc
    14941 gcacttctat tgcgaactag cgtcggcgga gaccatgcag tcgcggcccg actcggcgat
    15001 gcagtaccta gaatcggcgc tcaccgccca tcggaaagca gtccgtgcca gtgcgctgct
    15061 cggcgacctg cacgcgcagg ccgggcggtg gagccaggcg atcgaggcct ggcaaaagat
    15121 cgagcagcag aacccgccct atctcgcgct ggtgtcacag cgcctgatga acgcctacga
    15181 gaagctcggc cgccgtgacg aaggattgca gctgcttaag ggctatcttg agcaataccc
    15241 ctcgctcgat ctgctcgaca ccgtcatcaa actggtctcc gagctgcagg gagcgccggc
    15301 cgcacaggcg ctgctcaagg acgagttgcg gcgcaatccg acgctgctcg gcctcgacaa
    15361 gctgctcgag tcgcaactcg gtgtcgcgcc ggccgacaag cgctccgacc tcgagctgat
    15421 caagaacctg atcaacacgc atacccggcg tctcgcgcgc taccgctgcg agacctgcgg
    15481 cttcaaggcc cggcagttct actggcgttg cccggcctgc ggcggttggg agagctatcc
    15541 gccgcggcgt tccgaggaat tcgacctgtc gccctagcgg cgaaacattt tctggcagca
    15601 tcgggaaggt gcgctggcgc atcgcgtcca acaacactgt ggagtctcaa tgaaggcaag
    15661 gcaactggcg atacccgagg tcgtgctgat tgaacccagg gtcttcggcg acgatcgcgg
    15721 cttcttctac gagagctaca accgcgcggc attccgcgag gcgaccggcg tcgatcccga
    15781 cttcgtgcag gacaaccatt cgaagtcgca gcgcggcgtg ctgcgcggcc tgcactacca
    15841 gctgccgccg cgcgctcagg gcaagctcgt gcgcgtcgtg cagggtgaag tcttcgatgt
    15901 cgccgtcgac atccgtcgcg gttcgccgac tttcggcaag tgggtcggcg aatcactttc
    15961 ggcagaaaac catcgccaga tgtgggttcc accgggtttt gcgcatggct tcgttaccgt
    16021 ttccgagacg gccgagtttc tttacaagac caccgacgtt tatgcgcccg attgcgagcg
    16081 ctgcatcgcc tggaacgacc cgatgatcgg catcgactgg cggctgagcg gcgagccgct
    16141 gctgtcggcc aaggatcgcc agggcgtgtc gctcgctgcc gcagaagtgt tcggctgaac
    16201 gatggcagcg tgccaagcag gtagcatgga cagatgagcg aatcgggaga acagcacttg
    16261 gccaacgaga gcatgggtct gacgctagaa gccttcgtcg gcaacacacc actggtacgg
    16321 ctcgtccgcc tgcccggcga ggcgatcgcc aaccgcggca acgtggtgct ggccaagctc
    16381 gagggcaaca acccggcggg ctcggtcaag gaccggccgg cgctgtcgat gatcgcccga
    16441 gcccaggcgc gcggcgagat caagcccggc gatacgctgg tcgaggccac ctcgggcaat
    16501 accggcattg cgctggcgat ggcggcggcg ttgcgcggct tccgtatggt gctggtgatg
    16561 ccggagaacc agagcatcga gcgccgccag gcaatggcgg cctacggtgc cgagctggtg
    16621 ctgacgccga agtcgggcgg catggaactc gcccgcgacg tcgccgagcg gatggttaaa
    16681 gagggcaagg gcgtcatgct cgaccagttc gccaacccgg acaacccgct agcgcattac
    16741 gaaggcaccg gtcccgagat ctggcgcgac accaacggca aggttacgca cttcgtctcc
    16801 agcatgggca ccacgggcac gatcatgggc gtttcgcgtt tcctcaagga aaagaatccg
    16861 gcaatcgaga tcgtcggctg ccagcccgag gagggctcgc agattcccgg catccgcaag
    16921 tggcccgagg cctatctgcc gaagatattc gaccgcagcc gcgttgatcg cgtcgaaaac
    16981 gtttcgcagg ccgacgccga ggcgatgacg cgtcggcttg ccgccgagga agggatcttc
    17041 gccggcattt cctcgggcgg ggcgatcagc gtcgcgctgc gcctcgccgc cgagctcgac
    17101 aacgcggtca tcgtcgccat catctgcgac cggggcgacc gctacctctc gaccggcatc
    17161 ttccccggct gagttactcc gcaggaatcg acatgatccc gacgctgacc ttcgacctcg
    17221 agacgatccc cgacgtcgct ggcctgcgca cgctctacgg cctcgacgcg tcctcgcccg
    17281 atgcgctgcc cgacgccgag gtcgccgagt tggcattcca gcggcgccgc gcccagaacg
    17341 gcaccgactt cctcccgctg cacctgcatc gcatcgttac catctcctgc gcgctgcgtg
    17401 ccggcgatgc gttcaaggtc tggtcgctgg cggcgcccga gctcgacgag ggggcgatca
    17461 tccagcgctt cttcgacggt atcgagaagt tcacgccaca gatcgtcagc tggaacggcg
    17521 gcggcttcga cctgccggta ctgcactacc gcggcctgat ccacggcgtc gctgcgccgc
    17581 gctactggga cctcggcgac ggcgactacg gcgactcgcg cgaattcaag tggaacaact
    17641 acatcagccg ctaccacacg cgtcacctcg acctgatgga cctgctggcg atgtatcagc
    17701 cgcgcgccaa cgcaccgctc gacgagctgg ccaagctgat tggttttccc ggcaagctcg
    17761 gcatggacgg ttcgcaggtc tggtcggcct ggctcaacgg caaggccgcc gaaatccgcg
    17821 actactgcga gaccgacgtg gtcaacaccc acctggtcgc cttgcgtttc cgcaagatgc
    17881 gcggcgagct gagtgccgat ggctacgccg cggaggttga tctggtccgt cacagcctgg
    17941 cgcagctcga tcaaccgcac tggcgggaat ttctcgaggc ttggccttag tccgcggcca
    18001 catcgcacgc agtctcacgc cgtgcgactg cggtcggcga caatcacggc atgcctgaaa
    18061 tcgacccaag cgaaaagatg tctgacgcgc tggtgctctg cgccacacat cgcctggcgc
    18121 gtcgtctgtc gctggcgggc gagggtgcga ttcggcggct gcgtccggcg ctcaccgtcg
    18181 gccaatggct ggacgcgcga acagaagccg cgctgctcgg tggcgaaatg gctgcaccgc
    18241 cacggcgcct gtcgcgattg caggagcgcc tgctctggcg ccgggtgatc gacgaggcgc
    18301 tcgccggcga tgcgatcgcg gcgctgcttg accgcgatgg cctcgtcgag gccgcgatcg
    18361 aggccaacgc gctgattgaa acctggggcg ttgccgcggg cggcggccac aacgaggacg
    18421 cacggcagtt tctcgcctgg cgacaggcgt tccgtcgccg ctgcgatgcc ggtggctggc
    18481 gtgacgcggc gcgctatcag ggctggcaga taggctcggt cgtcgccggc gcgggattgc
    18541 cgaaggccgt gatgttcgcc ggctttgatc gcttcaaccc gcaggagcaa cgcctgctcg
    18601 atgcgctgag tggggctggg gttgcggttc gcgtcgagca gcctgatccc gccacgccgg
    18661 catcgcactg cctggctttc gacagcccgg atcgcgaact cgccgcagcg attgcctggg
    18721 cgcagggcgt cttcgtagcc cagcccgcgg cgcgcgtcgg catcgtcgtt cccgaactcg
    18781 cgaagcgacg ggcgacgctg ctgcgcgtcc tcgacgagac ttttgcgccg gcaacgatgc
    18841 gcgccgccgc tgccgatgca ccgcgaccct acaacctctc gctcggcgag tcgctcaacg
    18901 aggcgccggg gatcgccacc gcgctcgaac ttctgcgcct ggcgcttccc ggcgcgcggc
    18961 cgacccagca agaactgcgg accgtgctcg attcgccctg gtggggcggc ggcgtcgccg
    19021 agcggctcgg ccgcggccag ctcgacgccg atctgcgtga gcacctgccg cccgaagtcg
    19081 acggcgcgac gctgctgcgc cgaacctggc gtctgcacta ccgtgatggg ggcctcgaac
    19141 gcacggcgcg tagcctcgcc gatctcgcgg ccgagtcggc gcgctggcgt ggccggcagc
    19201 ccccctcggc gtgggccaca gccttcgcgg cggcgctcga tgcggcgggc tggcccggcg
    19261 agcgcgccgc gtcgagccgc gaattccagg cgcaagaagc cttcgccgaa acgctaaacg
    19321 cgctggccgc gctcgacgaa ttcgccggcc gggtcggtgc cggcgaggcc gtgacgctgc
    19381 tcgccgagca ttgccgcgag atgatcttcc agccgcgcac cgaaggtgcc ccgcgcctgc
    19441 aggtgctcgg cttgctcgag gccgccaccg aatccttcga cgcgctgtgg gtctgcggca
    19501 tgagcgatgc cgtctggccg ccgccggcac ggccgaaccc cttcctccat ccggccgcgc
    19561 agcgcgccgc cggcagtccc aatgcctcga gcgaggtgca gttccgtttc gccacggcga
    19621 ttcatgcgcg cctgctgcgt gccgcgccgg ccgtgcggtt ctcatgggcc atccaggacg
    19681 aggcgcgtgt gcaactgccc agcgccttgc tcggcgccga tccggctgct gcgctcgctg
    19741 ccgcagcaga gaccgccggt gcgtcggagc tcgccgcacg cttgcgcgcg caggccggcg
    19801 atgccgcgat cgagcaagtc gccgatcgct atggaccacc ggtcgccgcc ggcgaaaccg
    19861 ttcgtggcgg cacgcacctg atccgcacgc aggccgcctg cccggcctgg ggctactacc
    19921 agtaccgggt cggcgcacgg gccttgaaga caccggtcga aggactcgac agcgccggcc
    19981 gcggcaccat cgtccatgcc gtattcgaag ccttctggcg cgaccgcgat tcggccgggg
    20041 ttttcggctg ggatgaggcg gtgctggcga tgcgcatcgg cgaagctgtg acggccggca
    20101 ttgccgcttt cgaggacgag cgcggcgagc cgctgccgcc tcgcttccgc gcgctcgaga
    20161 cgacccgatt ggcggcgctg ctggccgagt ggctggcggt cgagcgccag cgaggcgtgc
    20221 ccttcgtcgt cgccggctgc gaggtcgagc gtcaattgag cctgcgcggg ctgccgatcc
    20281 gcgtcgtcgc cgatcgtgtc gaccggcttg ccgacggtcg cctcgtcatc atcgactaca
    20341 agaccggccg ggcgccggca ctgagcgcct gggcaacgca gcgcatcgtc gagccgcagc
    20401 taccgatcta cgccgccggg gaatccggag aaccggttgc tgcagtagcc ttcgccgacc
    20461 tgcgggcgcg gccgccgaag ttcgccggtc tcgccgccga gaacggcctg ctccccggcg
    20521 tcggcgacgt cgatggcgcg cggcgccact tcgacgccga cagtttcccc gactggcagg
    20581 ccgtgctggc gacctggcag gcacgcctgt tgatgctcgc cgacgaggtc cacgccggcg
    20641 atgccgcggt ccgcgtcgac aacccaaaga cacttgccta cgctgaggtg ctgccgctgc
    20701 tgcgcctcgc cgagcgtgat tggttgttcg gcgcggccgg cgccgacgga ggcgacgatg
    20761 cctgagtcga tgtgcgccgg gatgccgacg ctggccgacg acgccgccgc gcgcgagcgc
    20821 gcgctggagc ccggctcgtt catcgttgag gcgccggccg gtgccggcaa gaccgagctg
    20881 ctgacgcagc gatttctcgc gttgttggca cgcgtcgaat cgcccgaaga ggtggtggcg
    20941 atcaccttta cgcgcaaggc cgcagccgag atgcgtggcc gcatcgtcga caacctgatc
    21001 gccgcggcca gcgcgccgcg gcccgatgcg ccgcaccgcg cgcggacctg ggagctggcg
    21061 cggcaggcac tcgcccacgg cgccgcgcgc ggttggggac tcgagcggca accggcgcgc
    21121 ctgcgcgtgc tgaccatcga cgcgctgtgc atgctgctgg cgcggcaggc cccgctgctc
    21181 tcgcagatcg gcggcatcgc gtcggtcagc gacgacagcg atgcgctcta cgccgaagcg
    21241 gcgcggcgca gcctcgcgct gctcgaaggc gacgacaccg cacacgccga ctgcgtcgct
    21301 gcggcgctgg cgcatcttga caacgacgcg ggccgcctgc agcgcctgct cgcggcgatg
    21361 ctcgcgcggc gcgaccagtg gctgccgcac ggtcgaccgg tcgaggccgg agtcggcaac
    21421 cgcgcggcgc tggcggtcat cgtcgaagcg gcgctcgccg atgtcgcgag cacagtcgat
    21481 ggcggcgcgc aggcggcgtt gatgccactg ttcgccgagg cgctcgccaa tgcccccgag
    21541 gccacggccg cgcagctcgc gccgctggct gagtggcagc cgctgctggt cgccgcttgg
    21601 gacgagttgc cgcgctggca ggcactggcg cgctggctgc tgacgcagaa gggcgagttc
    21661 cgcaagcgct ggaccgccaa cgaaggcttt ccggcgggca aggccgggca gggcgccaag
    21721 gcggcgatcg gcgagtggat tgccgccctg ccggtgagcg ccgaggcggg cctggctgcc
    21781 gtgcaggcgt tgccgactcc cgaggatgcc gccgccgacc tgccgctggt cgaggcgctc
    21841 gcgcgcctgc tgtggcttgc ggttgcgcag ctctggctgg tcttcggcga aacccgcgag
    21901 gtcgacttca tcgccgtcgc cgaggcggcg ctgcgagcgc tcggcgagcg cgatgcgccg
    21961 agcgacctcg cactcgacta ccgtatcacc cacttgctcg tcgacgagtt ccaggacacc
    22021 agtccaagcc aggtcgagct acttcagcgg ctcaccgccg gctggggcga ggccgagggc
    22081 gatgagcgca cgctgttctg cgtcggcgat ccgatgcagt cgatctaccg tttccgcaag
    22141 gccgaggtcg ggctgttcat cgaggcacgg cgccgtggca tcggcagcat ccgcctggcg
    22201 ccactgcaac tggcgcgcaa caaccgcagc gacgaaggac tagtcgactg gatcaacgcg
    22261 acgctgccgg ccgcctttgc gtcggccgat gatccgctgc gcggcgccat tgcatatcgg
    22321 cggttcgtcg cgacgcgtga gggtcgtccc ggttcgggcg ccttctggca tccgttggtc
    22381 tgcgagcggg gcgtgcccgc gagcgccgtg gcgaccgccg aagccgggcg catcatcgcg
    22441 ctggtcgacg agttgcgcgc cagcgacggt gagcgcgaga tcgccgtgct ggttcgggcc
    22501 cgctcgcacc tcacggcgct ggtcgcgcaa ctgcgccgca accggcccga cctgcccttc
    22561 gaggccgtcg aaatcgattc gctggaaagc cggcagtggg tgcgcgactt gatcatcctc
    22621 gccagcgcgc tgggccagct ggccgaccgg gtgaactggc tggccttgct gcgctcgccg
    22681 ctctgcggcc tgacgctcgt cgacctgcac gcactggctg ccggcaccgg acggacctcg
    22741 ccctgggccc tgctgcacga cgatgcgcgg ctcgcgacgc tctcggccga tggccgcgag
    22801 cgggcactgg cgctgcgcgt ggtcatcggc gaggcgctcg cacacgccgg gcggcaacgg
    22861 cgtgcgcgct gggtcgaagg cgtctggctg gcgcttggcg gtgccgcgac ggttcccgac
    22921 gaggcggcgc tggccgactg ccgtgcctgc ttcgacctga tcgagcacct ttcggcggcc
    22981 ggccgcttcg atctcgatac cttgcccgcc gaggtcggaa agctcttcgc cgctgccgat
    23041 ccgcgtggcg ccgggcggct gacactgatg acgatccaca aggccaaggg cctcgagttc
    23101 gatacggtga tcctgcccgg cctgcagcgc gcgccgggcg gtttcgacga tccgccgctg
    23161 ttgcgctggg agagcgtcat cgccgaagac ggcacgcgtc acaccgccgt cgccgcattg
    23221 ccgccacgcg aaggggcggg cggtgaggcg agttatcgct tgctcgagcg gctcgagcgc
    23281 gaccgcggcc gggccgaggc agcgcgcgtc ctctacgtcg gcgcgacgcg cgcgaaacgc
    23341 gcgctgcacc tctttgccag tgtctggcag agcgccgacg gcgaggcgct ggcaccgccg
    23401 cgcggcgagt cgctgctcgg ctttctctgg ccggcggtcg ccgaggagtg ggccgcgctg
    23461 ccggtcgatc tggcggccga tgccgctgcg gcgggcgctg cgcagctggc tgatttcgtg
    23521 ccgccactgc gccgtctgtc gagcgccgga ttcgcggcac gtctttccgc gacgctaccc
    23581 ggtgacgtcg atgccggcag cggctgcgac gattcggcag cggcgcgcga tcaagcggta
    23641 atgcaccgca tcgactgggg ccgaggtgcc aatgcgcgcg aagggctgat cggcacgctg
    23701 actcaccgct atctcgagcg catcgccgtg gatggcctgg cggcgtggcc cgctgagcgt
    23761 tttgatggcc tgcacggtgc gatgcgcgac tggctggtcc gggaagggct gccaccgatc
    23821 gaggccgccg cgtcggcggc cgaggttgcg gcgatgctgc gcctcacgcg gcaaagcgcc
    23881 gatgggcaac gcgtgttgcg cgccaagggc gatgccgagc tggcactggt gagcgaagac
    23941 gccgacggcg cgcgcgtgca ggtcgtcgac cgcagtttca tcgacggcgg cgtgcgctgg
    24001 gtcgtcgact acaagaccga ggccgttggc gacggcaatt tcacggcagc cgccgcgcgc
    24061 caccgcgagc aactggcgcg ctacgcgaag ctgttcgccg gtgagagccg gcccatccgc
    24121 tgtgcggttt tcttcgttcg ctacggtcgc ctcgaaagct tgtccgatga atgaaaaccg
    24181 cggtgctggc gtgctgacag cggcttcgcc ggcgccggcg gccaacaaat cgcggctttc
    24241 tgtccatgcc ttgcccacaa atagcgcgag tcgcgagaca atacggggct atcactttcg
    24301 gacaatcaga tgaaacgggt cgacgattac cgactgaaat tcggcaagca tgagctggtg
    24361 cccatcatgg tcggcggcat gggcgtggac atctccaccg ccgagctgtc gctggaagcc
    24421 gcgcggctgg gcggcgttgg gcacatctcg gatgccatgg tgccgacggt atcggaccgc
    24481 cgcttcaaga ccaagtacgt caagaacaag cttaagcagt acaagttcaa cgtcgagaac
    24541 gccgacaagt cggtggtgca attcgaccta ggcctgctcg ccgaggcgac cgcgatgcac
    24601 gtcggccgca cgatggaggc caagcgtgga ccgggcctga tcttcatcaa ctgtatggag
    24661 aagctgacca tgaatgcgcc gaaagagacg cttcgggtcc gcatgtccag cgcgctcgac
    24721 gccggtatcg acggcatcac gctcgcagcc ggcctgcatc tcggctcctt cgcactgatc
    24781 gaagaccacc cgcgcttccg cgacgtcaag ctcggcatca tcgtttcctc gctgcgggcg
    24841 ctgcagctct tccttaagaa gagcgcgcgc accggccgcc tccccgacta cgtagtcatc
    24901 gagggaccgc ttgcaggcgg tcacctcggc ttcggcatgg actgggcgca gtacgatctg
    24961 gcgaagatcg tcatcgagat ccgcgactgg ctcgccgccg aaaagctaga cattccgcta
    25021 attccggccg gtggtatctt caccggcacc gacgccgtca acttcatgga aatgggcgca
    25081 gcggccgtgc aggtggcaac gcgcttcacc gtcagcgccg agtgcggctt gcccgaagac
    25141 gttcagcagg agtacttcaa ggccagcgaa gaggacatcg aggtgaacac catctcgccg
    25201 accggctatc cgatgcgcat gctcaagagc agcccggcta tcggcgacgg catccgcccc
    25261 aactgcgagg cctacggcta cctgctcgac gccaagggca actgccagta catcgaggcc
    25321 tacaaccgcg aggtggccgc gcatcccgag gcgaagaagg tcaaggtctt cgacaagacc
    25381 tgcctgtgca cgcacatgcg caacttcgac tgctggacct gcggccacta cacctaccgt
    25441 ctcaaggaca ccacggtgcg tgacgccgag ggcaaatacc agatcctctc ggccgagcac
    25501 atcttcaagg actaccagtt cagcaccgac ggcaagatcg cgctgccacc ggcggcgtaa
    25561 cggctgcgtc agcgcactcg acacggaagc ggccggccga tgccggccgt tttgcttggc
    25621 tgaccccggc tcgtgactag gcacgttgcc agccaagaat tgccgtctaa gccatccagc
    25681 gggaatcgac catgaaattg gtcatcagag cattcttcaa gacactgcgc gcggtactcg
    25741 gcccgccgat gctgc
//
LOCUS       DATDAA010000458         3638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_33456,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000458 DATDAA010000000
VERSION     DATDAA010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33456"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VIS73_13920"
     CDS             <1..531
                     /locus_tag="VIS73_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEY5764302.1"
                     /translation="LQERVAKRAGGVAVIKVGAATEVEMKEKKDRVDDALHATRAAVE
                     EGIVPGGGVALLRAQQAVAKLSGANADQDAGIKIVQRALEAPLRAIAANAGDEPSVIV
                     AKVHEGTGAFGYNAATGEYGDLVQMGVIDPTKVTRTALQNAASVAALILTTDATVAEA
                     PKEEKALPAPSPEMDY"
     gene            780..1721
                     /locus_tag="VIS73_13925"
     CDS             780..1721
                     /locus_tag="VIS73_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEY5764303.1"
                     /translation="MKSDLARSHLWTNRAQTLLLIGLLVALGGLAGFVLLGELGFWAA
                     TLAILLALVVEPVAASRLTLLLYGARPIPRGAAPQLWLMLDELARCAGLPATPLPYFV
                     PTRVINAFAVGNRRVSAIALTDGLLREMGGRELAGVLAHEVAHVANGDLRVMNLADYV
                     SRLTALLSLVGLALWIYYLPGFIAGTLDPPWIGLLLLSLSPHLALVAQLGLSRVREFA
                     ADLTAARLTGDPMGLAGALAKIERATRSWRSWVIPGWENANPSWLRSHPETTERIRRL
                     MAISGFERRDDWGAFVRPECGPPVTRRPRLVLTGIWR"
     gene            1831..2973
                     /locus_tag="VIS73_13930"
     CDS             1831..2973
                     /locus_tag="VIS73_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY5764304.1"
                     /translation="MGEIGLFRVRSAMKRHYWTGLIVLLVGLGLLALPDGLAKPGVRD
                     AVPRQVTPRGPLLSDELATVGLFEQTRDSVVYITTSAQVRDFWSRDVFSVPRGSGSGV
                     FWDGDGHIITNHHVIEGASEAKVTLADGSSYTAILVGSSPPHDLAVLRIGTGYKRPAP
                     IPIGTSHDLRVGQNVYAIGNPFGLDWTLTSGLISALDRSLSGGRGASIEHLIQTDAAI
                     NPGNSGGPLLDSAGRLIGINTAIYSPSGASAGIGFAVPVDTVNRVVPQLIRQGRYIRP
                     ALGIEADEGINRRLTEILGIKGVVILRVSPQSAAARAGLRGVTVSRHGDVVPGDIITA
                     IEGKPVDSLGRLVARLDDYSVGESIRVTVLRSGKTIDLRVTLQAGG"
     gene            complement(3035..>3638)
                     /locus_tag="VIS73_13935"
     CDS             complement(3035..>3638)
                     /locus_tag="VIS73_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764305.1"
                     /translation="RGHPQRRFSRTRFKPRGELVSSLEDLLAAVAGVALLCRRSEDPI
                     PPGGARPAVSEWAITDESPDGFGIRFLRGDMRRVEVGDLVAFRPRERGRIHLCLVRRI
                     TNAGAAQLELGLQEISPQAQIVALPAAPAGRGATQAIYLPKMPGFSNAAGLIVAPGTI
                     ELGQTISLAISGQSARMVAVRFLQRGERSHLIQIDPAPAF"
BASE COUNT          603 a         1122 c         1245 g          668 t
ORIGIN      
        1 cttcaggagc gtgtcgccaa acgggctggc ggtgtcgcgg tgatcaaggt cggcgcggcg
       61 accgaggtcg agatgaagga gaagaaggac cgcgtcgatg acgccttgca tgcaacccgc
      121 gctgcggtcg aggaaggtat cgtcccgggt ggcggcgtcg cgctgctgcg ggcacagcag
      181 gcggttgcaa aactgtctgg agccaacgcc gaccaggacg ctggcatcaa gattgtccag
      241 cgtgcgctgg aagccccgct gcgtgcgatt gccgccaacg ccggtgacga accatcggtg
      301 attgtcgcca aggtgcatga gggcaccggc gccttcggct acaacgccgc gaccggcgag
      361 tacggcgatc tggtccagat gggcgtgatc gatccgacga aggtgacgcg cacagccttg
      421 cagaacgccg cgtctgtggc tgccttgatc ctcaccaccg acgcaacggt cgccgaggcc
      481 ccgaaagagg aaaaggcctt gcctgcgccc tcgcctgaga tggactactg atccgtcccg
      541 cggcggcggc gtccggttgc ttcggaggcc gccgccgcag tttgcaaggg ttttccggga
      601 ttccgcgttg gtcaccgctt caaaacagcg gcccctgttg cacgtgaatt acggaacgaa
      661 catagtcaaa gcgatgattt tttctgggct tgccaccctg cttcgaggcc ttgacggcgc
      721 ccgcgccggc cgaacaggcg ggtgccgacg gaactaggcc tagcgctgcc gagcgaaaca
      781 tgaaatcgga cctcgcgcgc tcgcacctgt ggaccaatcg agcccagacc ttgctcctga
      841 tcgggctgct ggttgcgctg ggagggctcg ccggttttgt cctgcttgga gagctaggtt
      901 tttgggcagc aacgctggca attctgctcg cccttgtcgt tgaacccgtc gctgcctcgc
      961 ggctgacctt gctcctgtat ggcgcacgcc ccattccgcg tggcgccgcg ccgcagctct
     1021 ggttaatgct ggacgaactt gccagatgtg cgggcttgcc cgctacgccg cttccctact
     1081 tcgtgccgac gcgtgtcatc aacgcatttg cggtcggtaa ccgccgtgtg tcggcaatcg
     1141 cgctgaccga tggtctgctg cgggagatgg gtggccgaga actggctggc gtactggcgc
     1201 acgaggtcgc ccacgtcgcg aatggcgatc tgcgcgtaat gaatctggcc gattacgtga
     1261 gtcgcctgac cgcgctcctg tcgctggtcg gcctagcact ctggatctac taccttccag
     1321 ggtttatcgc cggcacgctc gatccgccct ggatcggcct gcttctgctc tcgctgtcac
     1381 cgcatttggc actggttgcc cagttgggtt tgtccagagt ccgcgaattt gcagctgatc
     1441 tgaccgcagc tcgtctaacc ggcgacccga tggggctcgc cggggcccta gccaagatcg
     1501 agcgcgccac acgcagctgg cgaagctggg tgatacccgg ctgggaaaac gccaatccct
     1561 cttggttgcg cagccatcca gaaacgaccg agcggattcg ccgtctgatg gcgatttccg
     1621 gattcgagcg gcgagatgac tggggcgcct tcgtgcggcc ggaatgcgga cccccggtga
     1681 cccgcaggcc gcggctggtt ctcactggga tctggcgtta gtgggtgacc agccgtgatg
     1741 gacgacggct cgacatgggg gaatcgtgaa cgggccgatt gaagtcttgc tgccgatgat
     1801 cgcattgctt gcggcgacgg tacgagggtc atgggtgaaa tcggattgtt tcgagtcaga
     1861 agcgcaatga aacgccatta ttggacgggg ttaatcgtgc tgctggtcgg gcttggcttg
     1921 ttggccttgc ccgatggcct ggccaagccc ggcgtacgcg atgccgtgcc ccgccaagtg
     1981 acaccgcgcg ggccgctgtt aagcgatgag ctggcgaccg tcggcttgtt cgagcagacg
     2041 cgagactcgg tggtctacat cacgacgagt gcacaggtca gggacttctg gtcgcgcgac
     2101 gtcttctcgg taccgcgagg ttccgggtct ggcgtcttct gggatggcga cgggcacatc
     2161 atcaccaacc accatgtgat cgaaggggcc agcgaggcaa aagtgacgct cgccgacggg
     2221 agttcctaca ccgccatcct ggtaggcagc agtccgccgc acgacctcgc cgtgttgcgc
     2281 atcggcactg gctataaacg ccctgcgcct attccaatcg ggaccagcca tgacctcagg
     2341 gttggtcaga acgtttatgc gatcggcaac ccattcgggc tcgactggac gctgacctcg
     2401 ggcctcattt cggccctcga tcgatccctg agcggcggac ggggggcgag catcgagcat
     2461 ctgatccaga ccgacgcggc gatcaatccg ggtaattcgg gtgggccgct actcgattcg
     2521 gccggtcgac tgatcggcat caacacggcg atctacagcc cgagtggcgc cagcgccggc
     2581 atcggtttcg cggttccggt tgatacggtc aaccgcgtcg ttccccagtt gattcgacag
     2641 ggtcgctata tccgcccggc gctcgggatt gaggcggacg aggggatcaa ccgtcgacta
     2701 acggaaatcc tggggatcaa gggtgtcgtc atcttgcgag taagcccgca gagcgcggcg
     2761 gcgcgcgccg gcctgcgggg cgtaaccgtg tcgcgccatg gcgacgtggt gccaggcgac
     2821 ataatcacgg cgattgaagg caaacccgtg gactcgctcg gcaggcttgt ggctcgcctt
     2881 gacgactatt cggtgggcga gtccattcgc gttaccgttt tgcggagcgg gaaaaccatc
     2941 gacctgcggg tgacgcttca ggcgggtggg tagcgggcaa ggcaatggca aaggccggga
     3001 acgccagcaa tattggtgac ccgcgaacgc tcgctcagaa ggccggtgcc gggtcgatct
     3061 ggataaggtg gctgcgctcg ccgcgctgga gaaagcggac ggcgaccatc cgcgccgact
     3121 ggccgctgat ggcgaggctg atcgtctgcc cgagctcgat cgtgccgggc gcgacgatga
     3181 gccccgccgc gttgctgaag ccgggcatct tcggcaggta gatcgcctgc gtcgcgcctc
     3241 gtcccgctgg cgcggccggc agcgcgacga tctgcgcctg cggcgagatt tcctgtagcc
     3301 cgagttcgag ctgcgcggcg ccggcgttgg tgatgcggcg gaccaggcag agatggatgc
     3361 ggccgcgttc gcgcgggcga aaggcgacca ggtcgccgac ctcgacgcgg cgcatgtcgc
     3421 cacgcaggaa gcggatgcca aagccgtccg ggctttcgtc agtgattgcc cattcgctga
     3481 ccgccggtct cgcgccgccc ggcggaatcg ggtcttcgct gcgccggcag agcagggcga
     3541 cgccggcgac cgcggcgagc aggtcctcga ggctggaaac cagttcgccg cgcggcttga
     3601 agcgcgtgcg cgaaaagcgt cgctgcgggt ggccgcgc
//
LOCUS       DATDAA010000459        13379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_173951,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000459 DATDAA010000000
VERSION     DATDAA010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 13379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13379
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173951"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VIS73_13940"
     CDS             complement(<1..250)
                     /locus_tag="VIS73_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY5764306.1"
                     /translation="MARNLDPKCRQCRREGDKLFLKGEKCFTDKCAIERRSYAPGQHG
                     QKSGARLSDYGVHLREKQKIRRIYGVLEGQFRRTFADAS"
     gene            complement(261..650)
                     /gene="rpsK"
                     /locus_tag="VIS73_13945"
     CDS             complement(261..650)
                     /gene="rpsK"
                     /locus_tag="VIS73_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY5764307.1"
                     /translation="MVKTATKVRKKVKKNVAEGIAHVHASFNNTIITITDRQGNALSW
                     ATSGGAGFKGSRKSTPFAAQVAAEAAGRAAQECGVKNLEVRIKGPGPGRESSVRALNA
                     LGLKITAITDVTPIPHNGCRPPKKRRI"
     gene            complement(666..1028)
                     /gene="rpsM"
                     /locus_tag="VIS73_13950"
     CDS             complement(666..1028)
                     /gene="rpsM"
                     /locus_tag="VIS73_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY5764308.1"
                     /translation="MARIAGVNIPNNQHAEIALTAIYGVGRTRAQKICDAAGVVRSAK
                     VKDLTEDQMERLRGEVTKFTIEGDLRREVTMNIKRLMDLGCYRGYRHRKGLPVRGQRT
                     RTNARTRKGPKKALSAKK"
     gene            complement(1092..1205)
                     /gene="rpmJ"
                     /locus_tag="VIS73_13955"
     CDS             complement(1092..1205)
                     /gene="rpmJ"
                     /locus_tag="VIS73_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEY5764309.1"
                     /translation="MKVFASVKRICRNCKVIRRKGVVRVICKDPRHKQRQG"
     gene            complement(1496..2830)
                     /gene="secY"
                     /locus_tag="VIS73_13960"
     CDS             complement(1496..2830)
                     /gene="secY"
                     /locus_tag="VIS73_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY5764310.1"
                     /translation="MLANAATIAKQGGKFADLKRRLWFLLGALLVYRIGAHIPVPGID
                     SAVLADLFNSQQGGILGMFNMFSGGALSRFTIFALGIMPYISASIIMQLMSHAVPYFE
                     AIKKEGEAGRRKITQYTRYGTVVLALFQAIGISIALESQAGLVMDPGFTFRLIAVTTL
                     VTGTMFLMWLGEQITERGLGNGISLIIFAGIVAGLPSAIGGLLELVRTGAMHPLTAIF
                     ICLLIVAVTYFVVFVERGQRKILVNYAKRQVGNKLYGGQSSHLPLKLNMSGVIPPIFA
                     SSIILFPATIAGMFGSGESGSWLKDAAAALSPGQPVYVMFYAAAIIFFCFFYTALVFN
                     SKETADNLKKSGAFVPGIRPGEQTARYIDKILMRLTLVGAAYITLVCLLPEFLILKWN
                     VPFYFGGTSLLIIVVVTMDFMSQVQAYVMSHQYESLLKKANFKGGGAGFPVK"
     gene            complement(2841..3278)
                     /gene="rplO"
                     /locus_tag="VIS73_13965"
     CDS             complement(2841..3278)
                     /gene="rplO"
                     /locus_tag="VIS73_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY5764311.1"
                     /translation="MELNTLKPAAGAKRNGKRVGRGIGSGLGKTCGRGHKGQKSRAGG
                     FHKVGFEGGQMPLQRRLPKRGFVSLTRDQKVEITLRALEQLAIDEVDMLVLRQARLIP
                     QAALSVKVVATGTLTRKVTLKGIGASKGAKAAIEAAGGAVVES"
     gene            complement(3280..3462)
                     /gene="rpmD"
                     /locus_tag="VIS73_13970"
     CDS             complement(3280..3462)
                     /gene="rpmD"
                     /locus_tag="VIS73_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY5764312.1"
                     /translation="MADKTIKVTLVRSIIGTKESHRATVRGLGLRRMNHTVQLEDTPA
                     VRGMINKVSYLLKCEG"
     gene            complement(3468..3992)
                     /gene="rpsE"
                     /locus_tag="VIS73_13975"
     CDS             complement(3468..3992)
                     /gene="rpsE"
                     /locus_tag="VIS73_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY5764313.1"
                     /translation="MAKNQGRKPQQSEDQGDGLREKMISINRVTKVVKGGRILGFAAL
                     TVVGDGDGGVGMGKGKSREVPVAVQKAMEEARRKLAKVQLKNGTLHHQVVGEHGASKV
                     LMMPAPEGTGIIAGGAMRAVFEVMGVTNIVAKAHGSTNPYNIVRATINGLMAMNTPAV
                     IAAKRGKTVAEIQG"
     gene            complement(4018..4371)
                     /gene="rplR"
                     /locus_tag="VIS73_13980"
     CDS             complement(4018..4371)
                     /gene="rplR"
                     /locus_tag="VIS73_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY5764314.1"
                     /translation="MDKKQSRLRRARQARAKIAELRAVRLSVHRTNQHMYAQVISPCG
                     SKVLASASTIEAEARKAVGNGATVKAAESVGKLIAERAKAAGVEVVAFDRSGFKYHGR
                     VKALAEAARAGGLKF"
     gene            complement(4386..4919)
                     /gene="rplF"
                     /locus_tag="VIS73_13985"
     CDS             complement(4386..4919)
                     /gene="rplF"
                     /locus_tag="VIS73_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY5764315.1"
                     /translation="MSRVAKNPVVIPAGVDVAIGAQEISIKGPLGTLKLAANPSVTVR
                     KDGAELVCVAAEGAENGNAMSGTFRALMANMVKGVTAGFERKLTLVGVGYRAQAQGDT
                     LNLTVGFSHPVAHKMPAGIKVETPTQTEIVIKGADKQQVGQVAAEVRAYRKPEPYKGK
                     GIRYANEVVVIKETKKK"
     gene            complement(4932..5327)
                     /gene="rpsH"
                     /locus_tag="VIS73_13990"
     CDS             complement(4932..5327)
                     /gene="rpsH"
                     /locus_tag="VIS73_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY5764316.1"
                     /translation="MSMSDPIADMLTRIRNAQLAEKATVAMPSSKLKIAIAEVLKDEG
                     YVEDFRVRDAEGKATLELGLKYYAGRPVIERVERVSRPGLRIYRGSTDIPRVMNGLGI
                     AIVSTPKGVMTDSKARAANVGGEVLCIVA"
     gene            complement(5344..5649)
                     /gene="rpsN"
                     /locus_tag="VIS73_13995"
     CDS             complement(5344..5649)
                     /gene="rpsN"
                     /locus_tag="VIS73_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEY5764317.1"
                     /translation="MAKLALINREKKRAKLVQKYAKKREALMVAIADETLGEVERYEA
                     RLKLQALPRNANPTRQRNRCQITGRPRGVFRKFGLCRNKLRELAFAGEVPGLVKASW"
     gene            complement(5657..6196)
                     /gene="rplE"
                     /locus_tag="VIS73_14000"
     CDS             complement(5657..6196)
                     /gene="rplE"
                     /locus_tag="VIS73_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY5764318.1"
                     /translation="MARLLEQFKTVVAPELIKKFGYKSTMEVPRITKITLNMGVGEAV
                     ADKKVLEAALGDMTKIAGQKPVTTKARKSIAGFKIRDGYPIGCMVTLRGPRMYEFLDR
                     LVSVALPRVRDFRGVSGKGFDGRGNYNMGVKEQIIFPEIEYDKIDALRGMNISITTTA
                     KSDAEAKALLAAFKFPFKN"
     gene            complement(6206..6523)
                     /gene="rplX"
                     /locus_tag="VIS73_14005"
     CDS             complement(6206..6523)
                     /gene="rplX"
                     /locus_tag="VIS73_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY5764319.1"
                     /translation="MEKIKKGDEIVVVTGKDKGKRGTVLRRAAADLLVVEGINRVKKH
                     VKPNPVKGVVGGIIDKELPIHISNVAVFNPATKKGDRIGIKQLEDGRRARFFKSNGEL
                     VDA"
     gene            complement(6533..6901)
                     /gene="rplN"
                     /locus_tag="VIS73_14010"
     CDS             complement(6533..6901)
                     /gene="rplN"
                     /locus_tag="VIS73_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY5764320.1"
                     /translation="MIQMQTMLDVADNTGARAVMCIKVLGGSKRRYAGIGDVIKVTIK
                     DAAPRGRVKKGDVYSAVVVRTAKGVRRPDGSLIKFDGNAAVLLNNKLEPIGTRIFGPV
                     TRELRNERFMKIVSLAPEVL"
     gene            complement(7037..7303)
                     /gene="rpsQ"
                     /locus_tag="VIS73_14015"
     CDS             complement(7037..7303)
                     /gene="rpsQ"
                     /locus_tag="VIS73_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY5764321.1"
                     /translation="MTEKIAVKRTLTGRIVSDKMNKSVTVLIERRVKHPVYGKVMVRS
                     QKYHAHDENNEAQTGDLVEITECRPISRTKTWVVTKLLQKAVIA"
     gene            complement(7300..7494)
                     /gene="rpmC"
                     /locus_tag="VIS73_14020"
     CDS             complement(7300..7494)
                     /gene="rpmC"
                     /locus_tag="VIS73_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY5764322.1"
                     /translation="MKASELKGKNAEALNAELIELLKAQFSLRMQHATQQLANTSQLS
                     KVRRDIARVRTILREKAVQQ"
     gene            complement(7499..7912)
                     /gene="rplP"
                     /locus_tag="VIS73_14025"
     CDS             complement(7499..7912)
                     /gene="rplP"
                     /locus_tag="VIS73_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY5764323.1"
                     /translation="MLQPARRKYRKEQKGKNVGLATRGAKVSFGEYGLKATARGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRIFPDKPISSKPAEVRMGNGKGNPEFYVAEIQPGKV
                     LYEMDGVDEALAREAFKLAAAKIPFATTFVTRHLG"
     gene            complement(7896..8777)
                     /gene="rpsC"
                     /locus_tag="VIS73_14030"
     CDS             complement(7896..8777)
                     /gene="rpsC"
                     /locus_tag="VIS73_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEY5764324.1"
                     /translation="MGQKISPTGFRLAVNRNWSSRWFASSKDFPSMLGEDYAVRQYLK
                     KKLAHASVGRILIERPAKNAKVTIFSARPGVVIGKKGEEIEQLRAEIARIMGVPVHVG
                     IEEIRKPELDAKLIADSIAQQLEKRIMFRRAMKRAMQNAMRLGAQGIKVMSAGRLNGA
                     DIARTEWYREGRVPLHTLRAEIDYATSEAKTTYGIIGIKVWVYKGEQASRADQRAAAL
                     PEAKADAPDAPKRTTRRVTKPATDAATETKAPAKRASAKKTAEAEGAEAPAAADGEKK
                     PAVKRVRKAGAKDAAAS"
     gene            complement(8787..9116)
                     /gene="rplV"
                     /locus_tag="VIS73_14035"
     CDS             complement(8787..9116)
                     /gene="rplV"
                     /locus_tag="VIS73_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY5764325.1"
                     /translation="METRAVLRGVRLSAQKGRLVADLVRGKPVDQALNILSFSPKKGA
                     VILKKVLESAIANAEHNDGADIDELKIKTVFVEKGMVLKRFSARAKGRANRISKPTCH
                     IYLTVGN"
     gene            complement(9129..9404)
                     /gene="rpsS"
                     /locus_tag="VIS73_14040"
     CDS             complement(9129..9404)
                     /gene="rpsS"
                     /locus_tag="VIS73_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY5764326.1"
                     /translation="MSRSLKKGPFVDGHLIDKIEAARGSNDKRPIKTWSRRSTILPEF
                     IGLTIAVHNGKQHIPVFVTENMVGHKLGEFSLTRTFKGHTAGKKAKK"
     gene            complement(9415..10245)
                     /gene="rplB"
                     /locus_tag="VIS73_14045"
     CDS             complement(9415..10245)
                     /gene="rplB"
                     /locus_tag="VIS73_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY5764327.1"
                     /translation="MALVKVKPTSAGRRAVVKVVNDNLHKGKPFAGLVESQSKHAGRN
                     NNGRITTRHKGGGHKQHYRLIDFKRNKDGVPGKVERLEYDPNRSANIALVLYADGERR
                     YIIAPKGTTVGQPIVSGSEAPIKSGNALPIRNIPVGTTIHCVEMTPGKGAQLARSAGT
                     SVQLLAREGTYAQLRLRSGEIRRVHVECRATIGEVGNEENNLRKLGKAGATRWRGIRP
                     TVRGTAMNPVDHPHGGGEGRTGEGGVPRSPWGQPAKGYRTRSNKRTDGMIVQRRHKKK
                     "
     gene            complement(10246..10560)
                     /gene="rplW"
                     /locus_tag="VIS73_14050"
     CDS             complement(10246..10560)
                     /gene="rplW"
                     /locus_tag="VIS73_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY5764328.1"
                     /translation="MTTTANQERLLQVLLAPQISEKATYIADKNEQVIFRVAPDATKP
                     EIKAAVELLFKVQVESVQVANVKGKEKRVGRNIGRRQGWKKAFVSLKPGQEINFVEGG
                     AQ"
     gene            complement(10557..11183)
                     /gene="rplD"
                     /locus_tag="VIS73_14055"
     CDS             complement(10557..11183)
                     /gene="rplD"
                     /locus_tag="VIS73_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY5764329.1"
                     /translation="MELKLVNDNGQGAATVQASDLLFGREYNEALIHQLVVAYQANAR
                     SGNRAQKGRSDIAKSTHKPWRQKGTGRARAGRASSPLWRGGGKTFPSSPDENFAHKVN
                     RKMFRAGMASILSFLAREGRLAVVESIAVDAPKTKLFAEKMRGLGYGAERVLVVTDTL
                     DENLYLASRNLENVLVLEAHETDPVSLIGFAKVLVTKNAVAKFEEMWA"
     gene            complement(11186..11830)
                     /gene="rplC"
                     /locus_tag="VIS73_14060"
     CDS             complement(11186..11830)
                     /gene="rplC"
                     /locus_tag="VIS73_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY5764330.1"
                     /translation="MSLGLVGRKVGMTRIFAEDGASIPVTVLDVANNRVTQIKTPETD
                     GYSAVQVTFGSRKPSRVNKAAAGHLAKAAVEAGEFLREFPIDSQQLASLKPGDQISVT
                     IFAAGQMVDVTGTSQGKGFAGVIKRHNFSSNRATHGNSVTTRAPGSIGMAQDPGRVFP
                     GKRMAGHLGAVRSTMQNLEVVRVDAERQLLLVKGAVPGSKGSDVIVRPAAKAGA"
     gene            complement(11948..12259)
                     /gene="rpsJ"
                     /locus_tag="VIS73_14065"
     CDS             complement(11948..12259)
                     /gene="rpsJ"
                     /locus_tag="VIS73_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY5764331.1"
                     /translation="MSNQKIRIRLKGFDYRLIDQSALEIVETAKRTGAVVRGPVPLPT
                     RIQRFDVLRSPHVNKTSRDQFEMRTHLRLMDIVDPNDKTVDALMKLDLPAGVDVEIKL
                     Q"
     gene            complement(12332..>13379)
                     /gene="tuf"
                     /locus_tag="VIS73_14070"
     CDS             complement(12332..>13379)
                     /gene="tuf"
                     /locus_tag="VIS73_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY5764332.1"
                     /translation="SIDSAPEEKARGITINTAHVEYETANRHYAHVDCPGHADYVKNM
                     ITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELV
                     EMEVRELLSKYEFPGDDTPIVKGSALKALEGDAGELGEGAIMALAEALDSYIPTPERA
                     IDGAFLMPVEDVFSISGRGTVVTGRVERGIVKVGEEIEIVGIRPTIKTICTGVEMFRK
                     LLDQGQAGDNIGALLRGTKREEVERGQVLAKPGSITPHTHFDAEVYILSKDEGGRHTP
                     FFNGYRPQFYFRTTDVTGSIELPAGTEMVMPGDNIGMKVKLIAPIAMEEGLRFAIREG
                     GRTVGAGVVAKIIE"
BASE COUNT         2412 a         4185 c         3904 g         2878 t
ORIGIN      
        1 gcgaggcatc ggcaaaggtc cggcgaaact gcccttcaag cacaccgtag atccggcgaa
       61 tcttttgctt ttcacgaagg tggacaccgt agtctgacag gcgggcgccc gacttctgac
      121 cgtgttggcc cggcgcgtac gaacggcgct caatggcgca cttgtcggtg aagcactttt
      181 cgcctttgag gaagagtttg tcgccctcac gtcggcattg acggcacttc ggatcgagat
      241 ttcttgccac gtcttgctcc ttaaatgcgg cgctttttcg gcggacggca cccgttgtgc
      301 gggatcggcg tcacgtcggt gatcgcggta atctttaggc caagcgcgtt aagcgcacgc
      361 accgaggatt cgcgtccggg accgggtcct ttgattcgga cctcgaggtt cttcacccca
      421 cactcctgcg cggcacggcc ggctgcctcg gcagcgacct gagcagcaaa gggagtcgac
      481 ttacgcgaac ccttgaagcc tgcaccgccc gacgttgccc aggaaagcgc gttaccctgg
      541 cggtcggtga tggtgatgat ggtgttgttg aaggaagcgt gaacatgcgc gatgccttca
      601 gcgacgttct tcttgacctt cttgcgaacc ttggtggctg tcttgaccat ttgcagtcgt
      661 cctcgttatt tcttggcgga aagcgccttc ttcggcccct tgcgggtccg tgcattggtg
      721 cgcgtgcgct gtccgcgaac gggcaatcct ttgcgatggc gataaccgcg atagcagccg
      781 agatccatca ggcgcttgat gttcatcgtg acttcacggc ggaggtcgcc ctcgatcgtg
      841 aacttggtca cttcaccgcg caggcgctcc atctggtctt cggtcaaatc cttgaccttg
      901 gccgaacgga cgactccagc ggcgtcgcat atcttctgtg cacgtgtccg gcctacgccg
      961 taaatcgccg tcaaagcgat ttccgcgtgc tgattgttgg gaatatttac cccggcaatt
     1021 cgagccattg actcacccca aatggcaaaa cgttaaattt taaatcacct gcgcttcacg
     1081 gacaatgccg atcagccctg gcgctgcttg tggcgcgggt ccttgcagat aacccggacc
     1141 acacctttgc ggcggatcac cttgcaattg cggcagatgc gctttactga tgcgaacact
     1201 ttcatgtcgt tctccaaatg tcgttgccgt cgcacagccg cgcgctcggt ttccgatctt
     1261 cgttactgcg tagagtccct catacggcgc gacaaccacc cgcgcctcca acaagtccta
     1321 cggtggaaaa agcaccgcaa tcctgttgcc cggcagaatc cggatgcatt acatccgcgc
     1381 cctgccggcg taaatttcgt gtactacatg cctggtctcg cgttccagcg cggcaatcgg
     1441 gaaatttcca tcgaacgccc gccagcctgc cgatgacgtc ttccgtcgcc accgctcact
     1501 taaccggaaa tcccgctcct ccgcccttga agttggcctt cttcaagaga ctctcgtatt
     1561 ggtgcgacat gacgtaagcc tgaacctgcg acatgaaatc catcgttacg accacaatga
     1621 tcagcagcga tgtgccaccg aaatagaacg gcacgttcca tttcagaatc agaaactccg
     1681 gcagcaagca aaccagcgtg atgtaagcgg ctccgaccag ggtcaggcgc atcaaaatct
     1741 tgtcgatgta ccgcgccgtc tgttcgccgg gacgaatacc cgggacaaag gcaccgctct
     1801 tcttcaggtt gtcggctgtt tccttggaat tgaagaccag cgccgtatag aagaagcaga
     1861 aaaagatgat cgctgccgca tagaacatca cataaacggg ctgaccgggc gacagcgctg
     1921 cagccgcatc cttcagccag ctgccggatt cgcccgagcc gaacatgccg gcaatggtcg
     1981 ccggaaacag gatgatggac gacgcgaaga tcggcgggat aaccccggac atgttgagct
     2041 tcagcggcag gtgcgagctc tggccaccgt agagcttgtt cccgacctgg cgcttggcgt
     2101 agttcaccag aatcttgcgc tggccacgct cgacgaagac cacgaagtag gtcaccgcaa
     2161 cgatcaacag gcagatgaaa atcgccgtca gcggatgcat tgccccggtg cggaccagct
     2221 caagcaggcc gccgatcgcg ctgggcaatc ccgcgacgat accggcgaag atgatcagcg
     2281 agatgccgtt gccaagaccg cgctcagtaa tctgctcgcc cagccacatg aggaacatcg
     2341 tgcccgtaac cagcgtcgtc accgcaatga ggcggaacgt aaagcccggg tccatcacca
     2401 gtccggcctg cgattcgagc gcgatcgaga tcccgatagc ctggaacagg gcaagcacga
     2461 cggttccgta acgcgtgtac tgcgtgatct tgcgccgccc ggcttcgcct tccttcttga
     2521 ttgcctcgaa ataaggcacc gcatgggaca tcagctgcat gatgatcgat gcagagatgt
     2581 acggcatgat tccgagcgca aagatcgtga atcgcgacag cgcgccaccc gagaacatgt
     2641 tgaacatgcc caggatgccg ccctgctggc tgttgaagag atccgccagt accgccgagt
     2701 cgatccctgg gaccggaatg tgcgcgccga tacgatagac gagcagcgcc cccaggagga
     2761 accatagacg acgcttcagg tcggcaaact tgccgccctg cttggcgatg gtagcggcgt
     2821 tagccaaaat tagctcccgc tcagctctcg acgaccgcgc cgccagcggc ttcgatcgca
     2881 gccttggcgc ccttgctggc gccgatgccc ttgagggtta ccttgcgggt cagcgtaccg
     2941 gtggcaacca ccttgaccga cagcgcagcc tgcggaatca agcgagcctg gcgcaaaacc
     3001 agcatatcga cttcgtcgat cgccagttgt tcgagcgcac gaagcgtaat ctcgaccttc
     3061 tggtcacgcg tcagcgaaac gaacccgcgc ttgggcaggc gacgctgcag cggcatctgg
     3121 ccgccctcga agccaacctt gtggaaacca ccggcgcggg acttctgacc cttgtggcca
     3181 cggccacatg tcttgcccag gccggaaccg ataccgcgtc cgacccgttt gccgttgcgc
     3241 ttggcgcctg cggcaggctt gagcgtattc agttccatct caaccctcgc acttcaaaag
     3301 gtaggacacc ttgttgatca tgccgcgaac cgccggcgtg tcctcgagtt gcaccgtgtg
     3361 gttcattcga cggagaccga ggccgcgcac cgttgcccgg tgggattcct tggtgccgat
     3421 gatgctgcga accagcgtca ccttaattgt cttgtcagcc attacgctta cccttgaatc
     3481 tcggcgaccg tcttgccacg cttggcagca atcaccgccg gcgtgttcat cgccatcagt
     3541 ccgttgatcg tcgcgcggac gatgttgtaa ggattcgtcg aaccgtgtgc cttggcgacg
     3601 atgttggtca cgcccatcac ctcgaatacc gcgcgcatcg cgccgccggc aatgatgccc
     3661 gtaccttccg gtgccggcat catcagtact ttcgacgcgc cgtgctcacc gaccacctga
     3721 tgatgcaggg tgccgttctt gagttggacc ttggccagtt tgcgacgcgc ttcttccatc
     3781 gccttctgga ccgcaaccgg cacttcacgg gacttgccct tgcccatgcc gacgccgcca
     3841 tcgccatcgc cgactacggt gagcgctgcg aaaccgagaa tccgtccacc cttgaccact
     3901 ttcgtgaccc ggttgatcga aatcatcttc tcccgcagac catcaccctg gtcttcagac
     3961 tgctggggct ttctgccttg attcttcgcc ataatgtcta gtctcccgca gcgtcaatca
     4021 gaacttgagt ccacccgccc gagctgcctc ggcgagggcc ttgacgcgac cgtggtactt
     4081 gaaacccgag cggtcgaacg cgacgacttc gacccccgcc gcttttgccc gctctgcaat
     4141 gagcttgcca accgattccg cggccttgac cgtggcgccg ttgccaactg ccttgcgcgc
     4201 ctccgcctcg attgttgatg ccgatgcgag aaccttgctg ccgcaggggg aaatgacctg
     4261 ggcgtacata tgctggttgg tccgatgcac gctgagccga accgcacgca attcggcgat
     4321 ctttgctctg gcctggcggg cacgacggag ccgggactgt ttcttgtcga tcatctgtat
     4381 gccccttact tcttcttcgt ttccttgatc accacgactt cgttcgcgta ccggattccc
     4441 ttgcccttat acggctcagg cttgcggtag gcacgaactt cggcggcaac ctggccgacc
     4501 tgctgcttgt cggccccctt gatgacgatt tcggtctgcg tcggcgtctc gaccttgatc
     4561 cctgccggca tcttgtgcgc gacgggatga gagaagccaa ctgtcaggtt gagcgtgtcg
     4621 ccctgtgcct gcgcccggta accgacgccg accagcgtga gcttgcgctc gaaaccagcc
     4681 gtgacgccct tgaccatgtt ggccatcagc gcccggaacg taccggacat ggcgttgccg
     4741 ttctcggcgc cttcggctgc cacacaaacc agctcggccc cgtccttgcg aacggtaacc
     4801 gacggattgg cagcgagctt cagcgtgccc aggggtccct tgatcgagat ctcctgcgct
     4861 ccgatggcga catcgacacc agcggggatg actaccggat tcttcgctac gcgcgacatg
     4921 tcctatcccc cttatgccac gatgcagagc acttcgccgc ccacattggc agcgcgcgcc
     4981 ttgctatcgg tcatgacacc cttcggcgtc gagacgatgg cgataccgag gccgttcatc
     5041 acccgcggaa tatcggtgct tccacgatag atacggagcc cgggacgcga gacacgttca
     5101 acgcgctcga tgaccggacg gccggcgtag tatttgagtc caagttccag cgtggccttg
     5161 ccttcggcgt cacggacgcg aaagtcctcg acgtagcctt cgtccttgag gacttcggcg
     5221 attgcgatct tcaatttcga agacggcatc gcgaccgtgg ccttctctgc cagctgggcg
     5281 ttgcggatgc gggtcagcat gtccgcaatg ggatcgctca tgctcattct atgtctctcc
     5341 tgcttaccag ctagccttga ccaagcccgg aacttcaccg gcaaatgcca gttcgcggag
     5401 cttgttgcgg caaaggccga acttccggaa aacgccacgc ggccggccgg tgatctggca
     5461 gcgattgcgc tggcgggtcg ggttggcgtt ccgcggaagc gcctgcagct tcagccgcgc
     5521 ctcatagcgc tcgacttcac ccagggtttc gtcagcaatc gcgaccatca gtgcttcgcg
     5581 ctttttcgcg tacttctgca ccagcttggc gcgcttcttt tcgcggttga tcagagcaag
     5641 tttcgccatg gtcgcctcaa ttcttgaacg gaaatttgaa cgcggcgagc aatgctttcg
     5701 cctcggcgtc ggatttcgcg gtggtcgtga tgctgatgtt catcccccgc agtgcgtcga
     5761 tcttgtcgta ctcgatctcc gggaaaatga tctgttcctt gacgcccatg ttgtagttac
     5821 cgcggccgtc gaagcccttg cccgagacgc cgcgaaaatc gcgaacgcgc gggagcgcga
     5881 cggagaccag gcggtcgagg aattcataca tgcgcggtcc gcgcagcgtg accatgcaac
     5941 cgatcgggta cccatcacga atcttgaagc cggcaatcga cttccgcgcc ttcgtggtca
     6001 ccggcttctg gccggcgatc ttggtcatgt caccgagggc tgcctcaagg acctttttgt
     6061 cggcaacagc ctcgccaacg cccatgttca gcgtgatctt ggtgatccgc ggcacttcca
     6121 tcgtcgactt gtaaccgaac ttcttaatca gttcgggagc gacaacagtc ttgaattgtt
     6181 cgagtaaacg cgccatgtcc gttccttatg cgtccaccag ttcgccattc gacttgaaga
     6241 agcgcgcacg gcgaccatcc tcaagctgct tgatgccgat ccgatcgcct ttcttggtcg
     6301 caggattgaa caccgcaacg ttcgagatgt gaatcggcag ttccttgtcg atgatgccgc
     6361 cgacaacgcc cttgaccgga ttcggcttga catgcttctt gacgcggttg ataccctcaa
     6421 ccacgagcaa gtcggctgca gcgcgacgca ggacggtacc gcgcttgccc ttgtccttgc
     6481 cggtgacgac gacgatctcg tcgcccttct tgattttttc catggcggct ccttacagga
     6541 cttccggcgc cagcgagacg atcttcatga agcgctcatt gcgcaattca cgcgtcaccg
     6601 gcccaaagat gcgggtgccg atcggctcga gcttgttgtt gagaaggacg gcggcgttgc
     6661 cgtcgaactt gatcagcgat ccgtcaggac ggcgaacacc cttcgccgtg cggacgacga
     6721 cggcgctgta aacgtcgccc ttcttcacgc gaccgcgcgg agcagcatcc ttgatggtga
     6781 ccttgatgac gtcgccaatt ccggcgtaac ggcgcttaga accgcccaac accttgatac
     6841 acatgactgc gcgcgcgccg gtgttgtcgg cgacgtcgag catcgtttgc atctgaatca
     6901 tttcaattca ctccaacttt tcccgcgatg ccgcgggcag tcttggaccc gaagggagaa
     6961 agcgccggcc gtgcgaccgg cgctcaaaga aggcgcgcat tgtacgcgcc attcaaacct
     7021 attgcaagcg ccttgttcag gcgataacgg ccttttggag cagcttggtc acgacccagg
     7081 tcttggtccg cgagatcggg cggcattcgg tgatctcgac cagatcgccg gtttgcgctt
     7141 cgttgttctc atcgtgtgcg tggtacttct gtgaacgcac catgaccttg ccgtaaaccg
     7201 gatgcttgac ccgccgctcg atcaaaacgg tcaccgattt gttcatcttg tcgctgacga
     7261 tacggccggt gagggtccgc ttgactgcga ttttctctgt cattgctgta ccgccttctc
     7321 gcgaagtatg gtccgcaccc gcgcgatatc gcggcgaacc ttgctcaact gactggtgtt
     7381 ggcgagctgc tgcgtcgcgt gctgcatccg caggctgaac tgcgccttaa gcagttcgat
     7441 cagctcggca ttcaacgctt cggcattctt gcccttgagt tcactggcct tcatcttctt
     7501 accccaagtg acgcgtcacg aacgtcgttg cgaagggaat cttcgctgca gcgagcttga
     7561 acgcctcgcg cgccaacgcc tcgtcgacac catccatttc ataaagcacc ttgcccggct
     7621 ggatctcggc gacgtagaac tccggattgc ccttaccgtt acccatccgc acttcggccg
     7681 gcttcgagga aatcggcttg tccgggaaga tacggatcca gattcggcca ccgcgcttga
     7741 tgtggcgcgt catggcgcga cgcgccgcct cgatttgccg agcggtcaat cgaccacgcg
     7801 cggttgcctt cagaccatat tcaccgaagg agaccttcgc gccccgggtc gcgaggccga
     7861 cgttcttacc cttctgttcc ttgcggtatt tccgcctagc tggctgcagc atctttcgct
     7921 cctgcctttc ttacccgctt gactgcgggc tttttctcac catcagccgc cgccggcgct
     7981 tccgcgccct ctgcttcggc agtcttcttg gctgacgccc gcttcgccgg cgccttggtt
     8041 tcggttgccg catcggtagc cggcttggtc acgcgacgcg tggtccgctt cggtgcatcg
     8101 ggtgcatcgg ccttggcttc cggcaatgcc gcagcgcgct ggtcggctct gcttgcctgt
     8161 tcgcccttgt agacccagac cttgatgccg atgatgccgt aggtggtctt cgcttcggag
     8221 gtcgcgtagt caatttcggc gcgtagcgta tggagcggca cgcggccctc gcggtaccac
     8281 tcggtgcggg cgatgtcggc gccattgaga cggccggcac tcatcacctt gatcccctga
     8341 gccccgaggc gcatggcgtt ctgcatcgcc cgcttcatgg cgcgacggaa catgatccgc
     8401 ttttcgagct gctgggcgat cgaatcggca atcagcttgg catcgagttc gggtttgcgg
     8461 atttcctcga taccgacatg gacgggaaca cccatgatcc tggcgatctc ggcgcgcagc
     8521 tgttcgatct cttcaccctt cttgccgatg acgacaccgg ggcgcgcact gaagatcgtg
     8581 actttggcat tctttgccgg gcgctcgatc aggatgcggc ccaccgaagc gtgagcgagt
     8641 ttcttcttca ggtactgtcg aaccgcgtag tcctcgccaa gcatggacgg gaagtccttg
     8701 ctcgacgcga accagcgcga tgaccagtta cggttgacgg ccaggcgaaa cccggtcgga
     8761 ctgatttttt gtcccatcgc ttttccttaa ttcccgactg tcaggtagat atggcaggtc
     8821 ggcttggaaa tccggtttgc ccggcccttg gcccgcgccg aaaagcgctt cagcaccatg
     8881 cctttttcta cgaagaccgt cttgatcttg agttcgtcga tgtcggcacc gtcgttgtgc
     8941 tcagcgttcg caattgcgga ctcgagaacc ttcttcagga tcaccgcacc cttcttcggt
     9001 gagaaagaca ggatgttgag cgcctggtcg acgggtttgc cacggacgag gtcggccacc
     9061 aagcggccct tttgggccga gagacgaacg ccacgaagta ctgcacgagt ttccatcatg
     9121 ggctcctgtt acttcttcgc cttcttgccg gcggtgtgac ccttgaacgt ccgggtcagc
     9181 gaaaactcgc caagcttgtg accgaccatg ttctcggtca cgaagacggg aatgtgttgc
     9241 ttgccgttat ggacagcgat cgtcagaccg atgaactcgg ggaggatcgt cgagcgacgc
     9301 gaccaggtct tgatcgggcg cttgtcattc gaaccgcggg cggcttcgat cttgtcgatg
     9361 aggtgaccgt cgacgaacgg cccttttttc agggaacgtg acatgcctta ccccttattt
     9421 cttcttgtga cggcgttgca cgatcatgcc gtcagtccgt ttgttggaac gcgtacggta
     9481 gcccttggca ggctgacccc acgggcttcg cggcacgccg ccttcaccgg tccggccttc
     9541 gccaccaccg tgcgggtgat cgaccgggtt catggctgta ccacgaaccg tcggtcggat
     9601 accgcgccaa cgggtcgcac cggccttgcc cagcttgcga aggttgttct cttcgttacc
     9661 gacttcaccg atcgttgcgc gacactcgac gtgtacgcga cggatttctc cggaacgcag
     9721 ccggagctgc gcgtaggttc cttcacgagc gagcagttgt actgaggtac ccgccgaacg
     9781 cgccagttgc gcacccttgc ccggcgtcat ttcgacgcag tgaatcgtcg ttccgaccgg
     9841 gatgttgcgg atcggcaggg cgtttccaga cttgatcggc gcctccgaac cgctgacgat
     9901 cggctgaccg acagtcgtgc ccttcggcgc aatgatgtag cgacgctcgc catcggcgta
     9961 gagcaccagc gcgatattgg cgctgcggtt cgggtcgtat tcaagacgct cgaccttgcc
    10021 gggaacgcca tccttgttgc gcttgaagtc gatcaggcga tagtgctgct tgtggccacc
    10081 gcccttgtgg cgggtcgtga tgcggccgtt gttgttgcgg ccggcatgct ttgactgcga
    10141 ctcgaccaga ccagcgaagg gcttgccctt gtgcaggttg tcgttgacga ccttgacgac
    10201 ggcacggcgg ccggccgaag tcggtttgac tttgacgaga gccatttact gagccccccc
    10261 ttcgacaaaa ttgatttcct ggcccggctt aagcgacacg aaggctttct tccagccctg
    10321 ccgacgaccg atgttccggc caacgcgctt ttccttgcct ttgacgttgg cgacctgaac
    10381 cgattcaacc tgcaccttga agagcagttc gacggcagcc ttgatttccg gcttggtcgc
    10441 gtcgggcgcg acacggaaga tcacctgctc gttcttgtcg gcaatgtaag tcgccttctc
    10501 cgagatctgc ggcgcgagca ggacctgaag cagacgctct tgattcgcgg tggtagtcat
    10561 gcccacatct cctcgaactt ggcgactgcg ttcttggtaa caagcacctt ggcgaaaccg
    10621 atcagcgaaa ccggatcggt ttcgtgcgcc tcaagaacca ggacgttctc caggttgcgc
    10681 gaagcgaggt aaaggttctc gtccagcgta tcggtcacga ccaggacgcg ctcggctccg
    10741 taacccaggc cgcgcatctt ctcggcaaac agcttggtct tcggcgcgtc gacagcgatg
    10801 ctctcgacca cggccagtcg gccttcacga gcgaggaacg acaggatcga cgccatgcca
    10861 gcacggaaca tcttgcgatt gaccttgtgc gcgaagttct cgtcgggtga agacgggaag
    10921 gtcttgccgc ctccgcgcca cagcgggctt gaggcacgac cggcgcgggc acggcccgta
    10981 cccttctgac gccagggctt gtgcgtggac ttggcgatgt cgctgcggcc cttctgggcg
    11041 cgattgccgg accgggcgtt ggcctgatag gcgacgacga gttgatggat cagggcttcg
    11101 ttgtactcgc ggccgaaaag cagatcggac gcctgcactg tcgcggcacc ttgaccgttg
    11161 tcattaacca gtttgagttc catgttcagg cccccgcctt cgccgccggc cgcacgatca
    11221 cgtccgaacc cttggagccc ggcacagcgc ctttgaccag cagcaactgg cgctcggcat
    11281 caacgcgaac gacttcaagg ttctgcatcg tgctgcgcac ggcgccgaga tgaccggcca
    11341 tgcgcttgcc cggaaagaca cgacccggat cctgcgccat gccgatcgag cccggcgcgc
    11401 gagtcgtcac cgagttaccg tgggtggcac ggttggaact aaagttatgg cgcttgatca
    11461 cgccggcaaa acccttgccc tgcgaggtgc cggtgacatc gaccatctgc ccggcggcga
    11521 aaatcgtcac gctgatctgg tcgcccggct tgagactcgc caactgctgc gaatcgatgg
    11581 gaaattcacg gaggaactca ccggcctcaa cggcggcctt ggccaaatga ccggctgccg
    11641 ctttgttgac acgcgagggc ttgcgcgaac cgaaggtaac ctgcacggcc gaatagccgt
    11701 cggtttccgg tgtcttgatt tgggtcacac gattgttggc gacgtccagc actgtcaccg
    11761 ggatgcttgc tccatcctcg gcgaaaatgc gagtcatccc aaccttgcgc ccgacaaggc
    11821 ctagactcat cttttttctc caaatcgccg gctacgattg gccgacgcac taaacaaaac
    11881 gtttttcgcg ctgccgacac ggggccggca ttgcggaaag ccgcggatta tagccgcggc
    11941 aagcgactta ttgcaacttg atttcgacgt cgacacccgc cggcagatcg agcttcatca
    12001 gcgcgtcgac ggtcttgtcg ttgggatcaa cgatgtccat caggcgcaga tgggtgcgca
    12061 tttcgaactg gtcgcgcgag gtcttgttga cgtgcggcga acgcagtacg tcaaaacgct
    12121 ggatgcgggt cggcagaggg accgggccac gaacgacggc gccggtgcgc ttggcagtct
    12181 cgacaatttc gagtgccgac tgatcaatca ggcggtaatc aaagcccttg agacggatac
    12241 ggattttctg gttcgacatg atctggtcca aagaacggtc aaagaacgaa acgggcgggt
    12301 gcgtcgatga cgactccgcc cgtaaaaggt attactcgat gatcttggcg acgacgccgg
    12361 cgccgacggt acgaccgcct tcacggatgg cgaagcgcag accttcttcc atggcgatcg
    12421 gcgcaatcag cttgaccttc atgccgatgt tgtcaccggg catgaccatc tcggtgccgg
    12481 ccggcagctc gatcgagccg gtgacgtcgg tggtccggaa gtagaactgc ggacggtagc
    12541 cgttgaagaa cggggtgtga cgaccacctt cgtccttcga caggatgtaa acctcggcgt
    12601 cgaagtgggt gtgcggcgtg atcgagccgg gcttggccag cacctgaccg cgctcgactt
    12661 cttcgcgctt ggtgccgcgc agcagggcgc cgatgttgtc gccggcctgc ccctggtcaa
    12721 gcagcttgcg gaacatttca acgccggtac agatggtctt gatggtcggg cggatgccga
    12781 cgatctcgat ttcttcgcca accttgacga tgccacgctc gacccggccg gtgacgacag
    12841 tgccgcgtcc ggagatcgag aagacgtctt cgacaggcat caggaaggca ccgtcaatgg
    12901 cgcgctcggg cgtcgggatg taggagtcaa gcgcctcagc cagcgccatg atggcgcctt
    12961 cgccgagctc gcctgcgtcg ccttcgaggg ccttgagggc ggagcctttg acgatggggg
    13021 tgtcgtcgcc ggggaattcg tacttggaga gcagttcgcg aacttccatt tcgacgagct
    13081 cgagcagttc ggcgtcgtca accatgtcgc acttgttcat gaagacgatg atgtacggca
    13141 cgccgacctg acgggcgagc aggatgtgct cgcgggtctg cggcatcggg ccgtcggctg
    13201 cggagacgac cagaatggcg ccgtccatct gcgccgcacc ggtaatcatg ttcttgacgt
    13261 agtcggcgtg tcccgggcag tcaacgtgcg cgtagtgacg gttggccgtc tcgtactcga
    13321 cgtgcgccgt gttgatggtg atgccgcgcg ccttctcttc cggtgcgctg tcaatcgac
//
LOCUS       DATDAA010000460         3163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_138772,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000460 DATDAA010000000
VERSION     DATDAA010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138772"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1848
                     /locus_tag="VIS73_14075"
     CDS             274..1848
                     /locus_tag="VIS73_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY5764333.1"
                     /translation="MEPSAVATLIVVALAAFVGGLALGRRRAAGRRAEAYFQRLGGKA
                     ADFSQALSPDPSDARLAEGFNAFLGKLQKMLGDVRSHTLSIAFQSNCVRKQMRDVSQR
                     AREQESLANEVYGASDASTTLLEKAAASAAHVADVTRGNLGDAREAYAELQDATQRIV
                     NVSDKVKVFQDNVGHLNDRSESIQNIVGVIKEISDQTNLLALNAAIEAARAGEAGRGF
                     AVVADEVRKLAEKVNKSTGQISENITGMRQQVAHIQTETNAIRDDTAHTREVIGKAAG
                     HFEKLVGDFEETTAALGGISDSVQTVAASNAQTHGHISQIRTHSREVVGHVAEAESAS
                     TELSREAEFVMRQVSRSRIGRGTLEKVLRLGEQFRDEVQAELQAVASSGVDVFDRNYR
                     PIANTDPQKFNTAYDDAVHQRVHGIMQRYLAEFPSCVFCLPVTADSYAPTHNRAEPLT
                     GNYEHDFTHNRAKRFFTSASEKRAAANTEPVLLQTYLRDTGEILSDIALPLTVGGRHW
                     GNVRIGMPTPALIDPT"
     gene            1967..2845
                     /gene="folD"
                     /locus_tag="VIS73_14080"
     CDS             1967..2845
                     /gene="folD"
                     /locus_tag="VIS73_14080"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEY5764334.1"
                     /translation="MTATLLDGNALARKLRAGFRERVDTLKLQGRTPGLAVVLVGEDP
                     ASQVYVRNKVNACGEIGMRSEKISFAADVEQAVVLRRIAELNADPAIHGILVQLPLPA
                     HFDADAVLEAIAPEKDVDGFHAENVGALVQGQPRFIPCTPYGVMKMLGETGISLRGAE
                     AVVIGRSNIVGKPMALLLLAAGATVTVCHSQTRDLAAHTRRADILVAAVGRPRFVTAE
                     MIKPGAVLIDVGINRIVDGPSAGKLCGDLDFDACRSVASWITPVPGGVGPMTITMLLA
                     NTLEAAERAGHSVKGE"
     gene            2847..>3163
                     /locus_tag="VIS73_14085"
     CDS             2847..>3163
                     /locus_tag="VIS73_14085"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEY5764335.1"
                     /translation="MAKKSTHPIVGLVMGSDSDWPVMQAAAMILKDFEVAYEARVVSA
                     HRTPDLLFDYAAMAGERGLRAIIAGAGGAAHLPGMAALTVGPSIGVPVTATPLQELNA
                     LLS"
BASE COUNT          587 a         1038 c         1025 g          513 t
ORIGIN      
        1 ctggcgcgca acgccggact cccgagggaa tacgggcggc agtcagcagc ctcgcgacgt
       61 cggatggagc ctagcggcga atggaggccg gccgatccgc catccagaac ctctccggac
      121 aacacacggt gaatagatgc cgtggatgcc ttgccgcgga tttcctaaac ggtctaaatc
      181 cgactatcct tcgccgacgc cgtcagccgc aaagccgcgc cgcagagcgc aaagagccaa
      241 cccatcgaac ggcgtggata tggaggaggg acaatggagc cgagcgctgt cgccacgctg
      301 atcgtcgtgg cactggccgc ctttgttggt ggtctcgcat tggggcgtcg ccgagccgcc
      361 gggcgtaggg ccgaagcata ttttcagcga ctcggcggca aggccgccga tttctcgcag
      421 gccctgtcgc ccgacccatc cgatgcccga ctcgccgaag gcttcaatgc ctttctcggc
      481 aagctgcaga agatgctcgg cgacgttcgc tcgcacacgc tgtcgatcgc cttccagtcc
      541 aactgcgttc gcaagcagat gcgcgatgtc agccagcgcg cccgcgagca ggagtcactg
      601 gccaatgaag tctacggtgc ctccgacgcc tcgacgacgc tgctggaaaa ggccgccgcg
      661 agcgctgccc atgtcgccga tgtcacccgc ggcaacctcg gcgacgcgcg cgaggcctac
      721 gccgaattgc aggacgcgac gcagcgaatc gtcaacgtct ccgacaaggt caaggttttc
      781 caggacaacg ttggccacct caacgatcgt tccgagagca tccagaacat tgtcggcgtg
      841 atcaaggaaa tctccgacca gactaacctg ctcgcgctca atgcggcgat cgaggcggcg
      901 cgtgcgggcg aggccggccg cggtttcgcg gtggtggccg acgaggtgcg caagctcgcc
      961 gagaaggtca acaagtcgac cgggcagatc tccgaaaaca taacggggat gcgccagcag
     1021 gtcgcgcaca ttcagaccga aacgaatgcg atccgcgacg acacggcgca tacccgcgaa
     1081 gtgatcggca aggcggccgg acacttcgag aaactggtcg gcgatttcga ggaaaccacg
     1141 gcggcgctcg gcggcatttc ggattcggtg caaacggtcg ccgccagcaa cgcccagacc
     1201 cacgggcaca tcagccagat tcgcacccac agccgcgagg tggtcgggca cgtcgctgag
     1261 gccgagagtg catcgactga gttgtcccga gaggccgagt tcgtcatgcg ccaggtgtcg
     1321 cgcagccgga tcggccgcgg tacgctggaa aaggtcctgc gccttggcga gcagttccgc
     1381 gacgaggttc aggcggaatt gcaggcggtc gccagcagtg gcgtcgacgt gtttgaccgt
     1441 aactaccgcc cgatcgccaa caccgatccg cagaaattca acacggccta cgacgacgcc
     1501 gtgcatcagc gtgtgcacgg catcatgcag cgctatctcg ccgagttccc gtcatgcgtc
     1561 ttctgcctgc cggtgaccgc cgacagctat gcgccgacgc ataatcgggc cgagccgcta
     1621 accggcaact acgaacatga tttcacccac aaccgcgcca agcgcttctt tacgtcggcc
     1681 tcggaaaagc gtgcggcggc caacaccgag ccggtgctgt tgcagaccta cctgcgcgat
     1741 accggcgaga tcctctccga tatcgccctg ccgctgacgg tcggcggccg gcattggggc
     1801 aacgtgcgga tcggcatgcc gacgccggcg ctgatcgacc ccacctaacc ggccggaagt
     1861 cgttcgcaga atcgggcagc gctggtaaaa ttcgcccgcc atgggaaagg cgggccgccc
     1921 cggcggccgc tttcccgttt tgtttttctg ctccagcgga gacgcgatga ccgccacact
     1981 gctcgatggc aacgcccttg cccggaaact gcgcgccggt ttccgcgagc gcgtcgatac
     2041 actgaaactg caggggcgga cgcccgggct ggcggtcgtg ctggtcggcg aagatccggc
     2101 ctctcaggtc tatgtgcgca acaaggtcaa cgcctgcggt gagatcggca tgcgctcgga
     2161 gaagatcagc ttcgccgccg atgtggagca ggcggtggtc ctgcgccgca tcgccgaact
     2221 gaacgccgac ccggcgattc acggaatact ggttcagttg ccgctgccag cgcatttcga
     2281 cgccgacgcc gtgctcgagg cgatcgcgcc ggagaaggac gtcgacggct tccacgccga
     2341 aaacgtcggt gcgctggttc agggccaacc gcgtttcatc ccctgcacgc cctacggcgt
     2401 gatgaagatg ctcggcgaaa ccggcatcag cctgcgtggc gccgaggccg tggtgattgg
     2461 ccgttcgaat atcgtcggca agccgatggc actgttgctg ctggcggccg gggcaaccgt
     2521 cacggtctgc cattcgcaga cgcgcgacct agcggcgcac acccgccgtg ccgacatcct
     2581 ggtcgcggcg gtcggcaggc ctcgcttcgt caccgccgag atgatcaagc ccggcgccgt
     2641 tcttatcgac gtaggcatca accgaattgt ggatggaccg agcgccggca aactctgcgg
     2701 cgacctcgac ttcgacgcct gtcgcagcgt cgcctcctgg attacgccgg tgccgggcgg
     2761 tgttggtccg atgacgatca ccatgctgtt ggccaacacg ctcgaagccg cagagcgcgc
     2821 aggccattcc gtgaaggggg agtgaaatgg cgaagaaaag cacgcatccg atcgtcggcc
     2881 tagtcatggg ttcagattcc gactggccag tcatgcaggc agcggcgatg atcctgaagg
     2941 atttcgaggt tgcctacgag gcgcgggtcg tgtcggcgca tcgcaccccc gatctgctct
     3001 tcgactacgc ggcaatggct ggcgagcgcg gtctccgggc gatcatcgcc ggcgccggcg
     3061 gcgcggcgca cctgccggga atggcggccc tgaccgtcgg gccgtcgatc ggcgtgccgg
     3121 tcacggccac gccgctgcag gaactcaatg cgctgctgtc gat
//
LOCUS       DATDAA010000461        12354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_33689,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000461 DATDAA010000000
VERSION     DATDAA010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 12354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12354
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33689"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VIS73_14090"
     CDS             complement(<1..511)
                     /locus_tag="VIS73_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY5764336.1"
                     /translation="MSRSLCLTALAATLATTISAAASDENAIVVTANRFSTKDVEAPY
                     ASEVHTRAQIESSGAVSLYDYLGKQTSVVVAPSFGNRASPRLDLRGYGIETGHQNVAI
                     IVDGVRLNGIDLATPLLGGIALDDVERIEISKGSGVVTHGDGAMAGTIQIHTRRALGS
                     SASAYAGNFG"
     regulatory      complement(634..841)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            982..1176
                     /locus_tag="VIS73_14095"
     CDS             982..1176
                     /locus_tag="VIS73_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764337.1"
                     /translation="MEAALDNLQQKVTRVAARCRDLHAENQTLRAQLATLAREHDALR
                     DKLDVASARLERLVEQLPEA"
     gene            1176..1484
                     /locus_tag="VIS73_14100"
     CDS             1176..1484
                     /locus_tag="VIS73_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY5764338.1"
                     /translation="MAAAKPALDVVLLGREYRVACQPDEAAALRQAVDALDARLRDIQ
                     SKSRGASTERIAVMAALNMTHEMLAAEQKNPAEISFDIEEARRRIDAMEAELDAVLKL
                     "
     gene            1513..1692
                     /gene="ssrS"
                     /locus_tag="VIS73_14105"
     ncRNA           1513..1692
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VIS73_14105"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1948..2922
                     /locus_tag="VIS73_14110"
     CDS             1948..2922
                     /locus_tag="VIS73_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY5764339.1"
                     /translation="MKKPVLIAAMMAIGVSLGSMSAPVSAQQKFVTIGTGGVTGVYYA
                     AGGAICRLMNKDRKTHGIRCSVESTGGSVYNINTIKAGELDFGVAQSDQQYNAIKGEG
                     AFKDSGAVSELRAVFSVHPEPLVVVGRKEAGAKSFGDFKGKRFNIGNPGSGTRATVDG
                     LMAAMGMKASDYSLASELKADEHGAALCDNKIDGFAYVVGNPAANIQDPTTTCGAQLV
                     NVTGPAVDKLVASYPYYAKATIPGGMYANNPDAVTTYGVVATFVSSANVSDDVVYAMV
                     KAVFDNFEDFKKLHPAFANLDPKDMIKNGNSAPLHNGAAKYYKEKGWM"
     gene            3033..5627
                     /locus_tag="VIS73_14115"
     CDS             3033..5627
                     /locus_tag="VIS73_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEY5764340.1"
                     /translation="MAQEKHHELSDEELRQIVAEADTGGRKPTGWVAKGLLITALAWS
                     LFQLWIASPLPFVVGHFVFNDTESRAIHLAFAVFLAYLAYPAFKSSPREYIPVVDWFL
                     AVGAAFAASYLFVFYQELSQRPGQPTTLDLVTAVCGLILLLEAARRVLGLPMVILATV
                     FIAYIFAGAYMPDMIAHKGASLGKGMNHLWLSTEGVFGVALGVSSGFIFLFVLFGALL
                     ETAGAGNYFIKSAISMLGHMRGGPAKAAVAASASTGLISGSSIANVVTTGTFTIPLMK
                     RVGYRPDKAAAVEVAASVDGQIMPPVMGAAAFLMVEYVGIPYTQVLKHAILPALISYI
                     ALFYIVHLEALKADIQGLPRREIKPWTSRLTNLLMTVSGLVILAGVVYWGLGWIKDIA
                     GDAAFIIISVLMAIAYVGLIGFAAKFPELHMDDPNEPLTELPEAGPTLKSGLHFLLPV
                     VALIWNLMVEELSPGLSAFWATCFMIFILVTQRPLFAFFRKHGNLATEFSRGWNDLFS
                     GLVTGARNMIGIGIATAAAGIIVGTVTLTGIGLVMTEFVEAVSGGNLMVMLFMVAVIC
                     LILGMGLPTTANYIVVSTLMAPVVVELGAQSGLLVPLIAVHLFVFYFGLMADVTPPVG
                     LASYAAAGIAKCDPIKTGVTAFGYSIRTAILPFMFIFNTQLLMIGIEGVGHFVVVVIG
                     AVAANLLFAAATQGWFLAKNRWWETIALLLICFTLFRPGFWMDEIYPPYVDAPASQIE
                     EIIAPMDKDGRLRMFIEGITLEGNEISKGVLLPLEKPGESPRARLADAGLTLMSGADG
                     SVQVLAVRFGSRAEKLGIEQGFEITKIELPADRPHKEWIFIPALLVLALIMVLQRRRL
                     AAAERKGV"
     gene            5627..10447
                     /locus_tag="VIS73_14120"
     CDS             5627..10447
                     /locus_tag="VIS73_14120"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEY5764341.1"
                     /translation="MAPEVVELISALQAKARERMGAAGDAAADFIAQYYRRLDPDDLP
                     NATTDDLYGAAMSHWQLLQKRKPGEARLRVLNPSVPEHGWQSAHTVIEIVNDDMPFLV
                     DSLTNEINRQGLTAHRVIHPVVEIQRDAKGAFKGIAADSKGSLRESVIHAEVDRRSDA
                     ESLASLSEGITRVLADVRAAVDDWQNMVGRVESAAQSLKAAPIKEAAGDRDEQVAFLH
                     WLSAGNFTLLGYRCYDLVGKKAGEALQIVPDSGLGILRGGDATPAASNSFASLAADLR
                     AQAHSPQALIVTKSNSRATVHRSGYLDYVGVKRFDAKGKVIGEHRFLGLFTSVAYSTS
                     PWQIPLLRRKAQQILELASFRPDSHAGKALKTIVDQFPRDELIQATVEELFETAMGIL
                     QLGERQRVRLFIRRDPYRRFYSCLVFVPRDRYDTALRVRIQNILMPVLGGVSVEHNVV
                     LSESVLARLQLIIRVDLGGEAATHDVRALEAKILEATRDWDDQLAAALVEAHGEERGG
                     EFVVRYRGAFPASYREQSSARTALFDVALVEVAASGGDGIAASLFVPPDASPHVLRLK
                     VAHVGGPLVLSDSMPMLENLGVRVVDEQPFTLRRRDGVVVCIHDFGLEHPTARDVQPL
                     DQLRALVHDAFLRVWRAEVENDRFNALVLAAGLDWRRVKVLRAYAKYMRQAGLSFSPQ
                     YIAATLCTHSGIAIDLARLFETCFDPAITDREPKVAEVEARIEAALANVENLDDDRIL
                     RQYLALIQATLRTSYYRREDGDVRHYVSFKLDPHRIPGLPEPRPKFEIFVFSPRFEGV
                     HLRGGSIARGGLRWSDRLEDYRTEVLGLVKAQLVKNAVIVPTGSKGGFVVKRPPVGGD
                     REALLAEGVSCYKTYLKGLLDVTDNLIDGKVVPPPDVVRRDGDDPYLVVAADKGTATF
                     SDIANGVAAEYGFWLGDAFASGGSVGYDHKKMGITARGAWESVKHHFRQLGVDTQSED
                     FTVAGVGDMSGDVFGNGMLLSKHIRLVAAFDHRHIFIDPDPDAATSYAERERLFALPR
                     SSWEDYDNKLISKGGGVWPRKIKSIPLSPEVRRVLGVEAKTLSSPELISAILRAPVDL
                     FYNGGIGTYVKASDESHAQVGDRANDGLRVDATELRCRVVAEGGNLGLTQRARIEFAA
                     HGGRIYTDAIDNSAGVDCSDHEVNIKILLGGIVAAGDMTLKQRDRLLEEMTDEVAALV
                     LQDNIRQTQVLSAMAARAPQLFGEQVRYMRELERAGRLIRRIEYLPNDEKIDERRQAG
                     EGLWLPELSVLLAYSKMDLYDGTLASNAPDDTVIAEALTSYFPKPLQKRFATQIAAHP
                     LRREIITTVVTNELVNRVGCTFAYRLTQETGADTGAIVRAYVLAREVFGLGALWHQLD
                     ALERLPADAQRSIGGALARQAERAALWFLRRPAVLEDLAASVKRFRPVVTAIDAALDK
                     GLQPVEYAAFDAAIKARTDAGVPADVARAVAALVPLHAALDITEVVSETGQNAVAVAG
                     AYFDLVGRLDLYWLSSRILELPASSRWEERARSALRDDLEAVVATLTAWIFRQCEIGN
                     KGCDAKALLDSWQQTRSTQLGRYQQMLGELKQGTPIDWAMISVLLRELKALSRI"
     gene            10494..10958
                     /locus_tag="VIS73_14125"
     CDS             10494..10958
                     /locus_tag="VIS73_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEY5764342.1"
                     /translation="MRYWLMKSEPSEVSIDDLAKMPKKTVPWFGVRNYQARNFMMNEM
                     AVGDGVLFYHSSCPQPGIAGICEVASTAYPDTTQFDAGSPYHDPKASEEKPRWYNVDV
                     QLVRKIPLIPLAALREEKALAEMLILKRGNRLSITPLTSAEWRHITGKMVAK"
     gene            10955..11749
                     /locus_tag="VIS73_14130"
     CDS             10955..11749
                     /locus_tag="VIS73_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY5764343.1"
                     /translation="MTWLFLYLVLGLVSGFFAGLLGIGGGLMMVPVLTIAFAAQGFPA
                     DELLHLALGTSMATILFTAIASLRAHHAHGAVMWPVVKSLTPGILLGTLLGTAIAGAV
                     PTRPLSIFFTAFVCLTAWQMATGFKPKPHREVPGTGGLIGVGSVIGAVSALVAIGGGA
                     LTVPFLTWCNVRVQHAIGTSAAVGLPIAIGGTIGYVFNGWSHQLPEWSAGFVYLPAVA
                     ALIVGSMSTAPLGAKTAHRLPVPTLKRIFAGLLLVLAGKMLWNLFA"
     gene            complement(11765..>12354)
                     /locus_tag="VIS73_14135"
     CDS             complement(11765..>12354)
                     /locus_tag="VIS73_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5764344.1"
                     /translation="DIDEFDEARATPEAALAYHAEAVAPALQALADFPVPTIAEIRGA
                     CVGGGLEIACCCDLRIAAPNARFGVPINRLGFSMYAGEMALVLRTVPRPVLAELLFEG
                     RLLAADEALAKGLLTRVVAADALTDEIRATAERIAAGAPLVARAHKHWLGRLADGHSP
                     SVEECAESLGTLNTADYREGLAAFREKRGPRFSGE"
BASE COUNT         2146 a         3856 c         3985 g         2367 t
ORIGIN      
        1 cgccgaagtt gcccgcgtag gccgatgcgc tgctaccgag agcgcggcgc gtatgaatct
       61 ggatcgttcc cgccatggcg ccgtcgccat gggtgacgac gccgctgcct ttcgaaatct
      121 cgatgcgttc gacgtcgtcg agcgctatgc cgcccagcag cggagtggcg aggtcgatgc
      181 cattgagccg caccccgtcg acgatgatag cgacgttttg gtggccagtt tcgatgccgt
      241 agccacgcag gtcgagcctt ggtgaagcac gattgccgaa cgacggtgcg acgaccaccg
      301 aggtctgctt gccgaggtaa tcgtagagtg aaacggcgcc cgacgattcg atctgcgcgc
      361 gcgtgtggac ttccgaggcg tagggagctt cgacgtcttt ggtcgagaag cggttggcgg
      421 taacgacgat cgcgttctcg tcgctggcgg cggcggaaat agtggtggca agggtggcgg
      481 ctagcgccgt gagacaaagg cttcgagaca tgtggttccc caagggcccg tcaggcgtcc
      541 ccgcgagacg ggtggttgtc gtatgaagcg cctagggatt cgtcgagcgt gccggcgtag
      601 cggcggtcac ccgtgccgca tccccgcagc acttccgctg gtctcacacc gggccggtct
      661 ccgggctcgc aagtcttgcc gcgccgcctt cccgaaccga gattcagtgg cgtgatggcg
      721 cggccgcact tgcttaccgt tgcgggggca gcagcgggtt tgcgaccagg gtcgcgcacc
      781 gctttcccgt ttcaccgtca gagggattcc tgtcgacggc acccgaggtg aagcgccccc
      841 tcatagacgg ggcgcgcgaa ttatacggtc gggccgccgt ttcgggcgag ggcggcgctg
      901 ggcaggagtg caataaagta catacaaaag cggcgcttaa tccttgaccg taaaaccttt
      961 tcgtacctat agtgaccgcc gatggaagcc gctctcgaca acctccagca gaaagtcacg
     1021 cgcgtcgcgg cgcgttgtcg cgacctgcat gcagagaacc agaccctgcg tgcgcagctc
     1081 gcgacgctgg cgcgcgaaca cgatgcgctg cgcgacaagc tcgatgtcgc aagcgcccgg
     1141 ctcgagcggc tggtcgaaca gctgccggag gcgtgatggc cgccgcgaag cccgcactcg
     1201 atgttgttct gctcggccgc gagtaccgag ttgcctgtca accggacgag gctgctgcat
     1261 tgcgtcaggc ggttgacgcg cttgatgcgc gactgcgcga tatccagtca aagagccgtg
     1321 gcgcaagcac cgagcgaatt gccgtgatgg cggcactgaa catgacgcat gagatgcttg
     1381 ccgcagagca gaaaaatccg gctgaaattt cttttgacat cgaagaggcg aggcgtagaa
     1441 tcgacgccat ggaagctgag cttgatgcgg tgttgaagct ctagaagctg catcagacaa
     1501 ggcttccgcg aagttcccct gcggtgtttg tcaaggtcat agaatccttg agccaataac
     1561 cttaggcgtc ggctgccgac catcgaagcc gctgttgtgc gcgccccttg cgggtgtgcc
     1621 tgaagcggaa ggaaagcggc ccatctgaac tccggttcag gatgccggcc tgacggcaca
     1681 ggcgggggac ccataccaag aagcgcgacg tgagttcacc gtcgcgcttt ttcatttcat
     1741 tttcgcgcgg tggcgtacgc ttggatcgat cgtcgcacgg cgattctgcg cggggcgaca
     1801 tttgaactcg cgcggcgata ggcggtcaat tcttcgctgg gtaaattcgc ggtgtatgct
     1861 gaataggcac accggcgcga cgccggcggc gatgcagcaa cggacacccg cgtccgcaca
     1921 acaatagtca taagacaagg agattccatg aaaaagcctg tactcattgc agcaatgatg
     1981 gcgatcggcg tatcgcttgg cagcatgtcg gcgcccgtat cggcacaaca gaagttcgtg
     2041 acgatcggaa ccggtggcgt gaccggtgtc tactacgctg ccggcggcgc gatctgtcgc
     2101 ctgatgaaca aggatcgcaa gacgcacggc attcgttgct cggtcgagag caccggcggt
     2161 tcggtctaca acatcaatac gatcaaggcc ggtgaactcg atttcggtgt cgctcagtcg
     2221 gatcagcagt acaacgcaat caagggcgaa ggcgccttca aggacagcgg tgcggtgtcc
     2281 gaattgcgcg ccgtattctc tgttcatccc gaaccgctgg tcgtcgtcgg tcgcaaagaa
     2341 gctggtgcca agtcattcgg cgacttcaag ggcaagcgct tcaacatcgg caacccggga
     2401 tccggcacgc gcgccacggt tgatggtctg atggccgcga tgggcatgaa ggcaagcgac
     2461 tactcgctgg cctccgagct caaggccgac gagcacggcg cggcgctctg tgacaacaag
     2521 atcgacggtt ttgcctacgt tgtcggcaac ccggcagcca acatccagga tccgacgacg
     2581 acgtgcggcg cacaactggt caacgtcacc ggtccggcag tcgacaagct cgttgctagc
     2641 tacccgtatt acgccaaggc gacgatcccg ggcgggatgt acgccaataa tcccgatgcc
     2701 gtgacgacct atggcgtggt cgcaaccttt gtcagttcag ccaatgtttc ggatgacgtc
     2761 gtctatgcga tggtcaaggc cgtcttcgac aacttcgagg acttcaagaa gctgcacccg
     2821 gcctttgcca atcttgatcc gaaagacatg atcaagaacg gcaattcggc gccgctgcat
     2881 aacggtgccg ccaagtacta caaggagaaa ggctggatgt aatttcggcc ggctcagcag
     2941 gaacggcccc gacctcgggg ccgtttttgc ttgcgggctt cgtatgggtt gacctgcgcc
     3001 atggcgtctg gcgcacctga ttggaggagg ctatggctca agaaaaacat cacgaactga
     3061 gcgacgagga actgcggcag atcgttgccg aagctgatac aggcggccgc aagccgacgg
     3121 gttgggtagc caaggggctg ttgatcaccg cgctcgcgtg gtcgctgttc cagttgtgga
     3181 ttgcctcgcc cttgccgttt gtcgttgggc atttcgtctt caacgatacc gagtcgcgtg
     3241 cgatccatct cgccttcgcg gtatttctgg cctatctcgc ctacccggcc ttcaagtcgt
     3301 cgccgcgcga atatattccg gtcgtcgact ggtttctggc tgtcggcgcc gccttcgccg
     3361 cctcctatct cttcgtcttc tatcaggaac tctcgcagcg gccggggcag ccgaccacgc
     3421 tcgaccttgt caccgccgtc tgcggcctga tcctgctgct tgaagcggcc cggcgcgtgc
     3481 tcggtctgcc gatggtgatc ctggcgaccg tgttcatcgc ctacatcttt gccggggcct
     3541 acatgcccga catgattgcg cacaagggcg cctcgctcgg caaaggaatg aaccacctgt
     3601 ggctgtcgac ggagggcgta ttcggtgtcg cgctcggcgt gtcgtcgggc ttcatcttcc
     3661 tcttcgttct gtttggcgcg ctgctggaaa cggctggcgc cggcaactac ttcatcaagt
     3721 cggcgatctc gatgctcggg cacatgcgag gcggcccggc gaaggcggcg gtggctgctt
     3781 cggcttcgac cggactgatc tcgggctcgt cgatcgccaa cgtggtcact accggcacgt
     3841 tcacgattcc gctgatgaag cgggtcggct atcgtccaga caaggcggcc gccgtagaag
     3901 tcgcggcctc ggtcgatggc cagatcatgc cgccggtcat gggcgcagcg gccttcctga
     3961 tggttgagta cgtcggcatt ccctacacgc aggtgcttaa gcacgcgatc ctgccggcgc
     4021 tgatttcgta tatcgcgctg ttctatatcg ttcaccttga agcgctgaag gccgacattc
     4081 agggattgcc gcgccgcgag atcaagccgt ggacttcgcg gctgaccaat cttctgatga
     4141 cggtctcggg tttggtcatc cttgccggag tcgtgtactg gggcttgggc tggatcaagg
     4201 acatcgctgg cgacgccgca ttcatcatca tcagcgtcct gatggccatc gcctacgtcg
     4261 gcctgatcgg tttcgcggca aagtttcccg aactgcacat ggatgatccg aacgagccat
     4321 tgaccgagtt gcccgaggcg gggccgacac tcaagtcggg gctgcacttc ctgctgccgg
     4381 tcgtcgcgtt gatctggaac ctaatggtcg aggagctttc gccgggcctt tcggcattct
     4441 gggcgacctg cttcatgatc ttcatcctcg tcacccagcg tccacttttt gcgttctttc
     4501 gcaagcacgg gaatctcgca acggagtttt cgcgcggctg gaacgacctg ttcagcggcc
     4561 ttgtaaccgg cgcccgcaac atgatcggca tcggcatcgc cacggcggca gcaggaatca
     4621 tcgtcgggac cgtgacactc accggcattg gtttggtgat gaccgagttc gtcgaagcgg
     4681 tttccggcgg caacctgatg gtgatgctgt tcatggtcgc ggtcatctgc ctgattcttg
     4741 gcatgggcct gccgacaacg gccaactaca tcgtcgtctc gacgctgatg gcgccggtgg
     4801 tcgtcgagct tggtgcgcaa agcgggttgc tcgttccgct gattgccgtc cacctgttcg
     4861 tcttctattt tggcttgatg gccgacgtga cgccgccggt gggtctggcc tcctacgcgg
     4921 cggcaggtat cgccaagtgc gatccgatca agaccggcgt tacggccttc ggctattcga
     4981 tccgcacggc gatcctgccc ttcatgttca tcttcaacac gcagttgttg atgatcggga
     5041 ttgagggtgt cgggcatttc gtggtggtgg tcatcggagc cgtcgcggcc aatctactgt
     5101 ttgcggcagc gacgcagggc tggttccttg ccaagaaccg ctggtgggaa acgatcgcgc
     5161 tgctactgat ttgtttcacg ctgttccgtc cgggcttctg gatggacgag atctatccac
     5221 cctacgttga cgcgccggcc agccagatcg aggagatcat cgcgccgatg gacaaggatg
     5281 ggcgcctgcg catgtttatc gagggcatca cgctcgaagg caacgaaatc agcaagggcg
     5341 tcttgttgcc cctggaaaaa cctggcgaaa gcccgcgtgc ccgtctggcc gatgccggac
     5401 tgacgctgat gtccggtgcc gacggaagtg tgcaggtgct ggcggtccgg ttcggcagcc
     5461 gagccgagaa actcggcatc gagcagggct ttgagatcac caagattgaa ctgccggcgg
     5521 atcggcccca caaggagtgg atattcattc cggcgctgct tgttctggca ctgatcatgg
     5581 tgcttcaacg gcggcgcctg gcggctgcag aacggaaggg cgtctgatgg cacccgaagt
     5641 cgtagaactg atttccgcgc tgcaggccaa ggcacgcgaa cggatgggcg cggccggtga
     5701 tgcggcagca gatttcattg cgcagtatta ccgccgcctc gatccagacg atctacccaa
     5761 cgccactacc gatgacctct atggcgcggc gatgtcgcac tggcaactgc tgcagaaacg
     5821 caagcccggc gaggcccgcc tgcgcgtcct caatccgagc gtgcccgagc atggctggca
     5881 gtcggcgcat acggtaatcg agatcgtcaa cgacgacatg cccttcctcg tcgattcgct
     5941 gaccaatgaa atcaatcggc agggcttgac cgctcatcgt gtcattcatc cggtggtcga
     6001 aattcagcgc gatgccaagg gagcgtttaa gggcatcgct gccgacagca agggttcgct
     6061 gcgcgaatcg gtgatccatg ccgaagtaga tcggcgcagc gatgccgaat cgctggccag
     6121 cctgagcgag ggcatcaccc gggtgctcgc cgacgtccgc gcggctgtcg atgactggca
     6181 gaacatggtc ggtcgcgtcg agtcggcggc gcagtcgctg aaagcggccc cgatcaagga
     6241 ggcggctggc gaccgagacg aacaggtggc ctttctccac tggctgtcgg ccggcaactt
     6301 cacgctgctc ggttatcgct gctacgacct agttgggaaa aaggcgggcg aggctctgca
     6361 gatcgttccc gattcgggtc tcggcatcct gcgcggtggc gacgcgacac ccgcggcttc
     6421 gaacagcttc gccagcctcg cggcagacct gcgagcgcag gcgcactcgc cgcaggcatt
     6481 gatcgttacc aagagcaaca gccgcgcgac ggttcatcgt tcagggtatc tcgactacgt
     6541 cggcgtcaag cgcttcgatg ccaagggcaa ggtcatcggc gagcaccgat tcctcggcct
     6601 gttcacctcg gtcgcctaca gcacgagtcc gtggcagata ccgctgctgc gacgcaaggc
     6661 gcaacagatt ctcgaactcg cgagcttccg tccagacagc catgccggaa aggcgttgaa
     6721 gacgatcgtt gaccagtttc cgcgtgatga gctgatccag gcgaccgtcg aagagctgtt
     6781 cgagacggcg atgggcatcc tgcagcttgg cgagcggcag cgcgttcggc tgttcatccg
     6841 tcgcgacccg tatcgacggt tctactcctg cctcgtcttc gttccgcgcg atcgttacga
     6901 cacggccttg cgtgtacgca tccagaacat cctgatgccg gtcctcggtg gcgtttcggt
     6961 cgagcacaac gttgtgctct cggagtcggt tctggcccgc ctgcaactga ttatccgcgt
     7021 cgatctgggt ggcgaagcgg caacgcacga cgtgcgcgca ctcgaggcga agattctcga
     7081 ggcgacccgc gactgggacg accagctcgc cgcggcgctg gtcgaagctc acggcgagga
     7141 gagaggtggc gagtttgtcg tgcgttaccg cggggcattc ccggcgagct accgcgagca
     7201 gtcgtcggcg cgcacggcgc tgttcgacgt tgcgctggtc gaagtcgccg cgagcggcgg
     7261 tgacggcatc gctgcgagcc tgttcgtgcc acccgatgcg tcgccacacg tattgcggct
     7321 caaggtggcg cacgtcggcg ggccgctggt gctatccgac agcatgccga tgctcgaaaa
     7381 tctcggcgtg cgggtcgtcg atgagcagcc gtttacgctt cgtcgccggg acggcgtcgt
     7441 cgtctgcatc catgacttcg gtctcgagca cccaactgcg cgcgacgtgc agccgctcga
     7501 ccaactgcgc gcgttggtcc acgacgcgtt ccttcgggtg tggcgtgccg aggtcgagaa
     7561 cgaccgtttc aacgcgttgg tgctcgccgc cgggctcgac tggcggcggg tcaaggtatt
     7621 gcgggcctac gccaagtaca tgcgacaggc gggtctcagc ttcagcccgc aatacatcgc
     7681 tgcgacgctg tgcacgcatt cgggaatcgc catcgatctc gcgcggctgt tcgaaacgtg
     7741 tttcgatccg gcgatcaccg atcgcgagcc gaaagtcgcc gaagtcgagg cgcgtatcga
     7801 ggcggcgctg gccaacgtcg agaacctcga tgacgatcgg atcctgcgcc agtatctggc
     7861 gctgattcaa gccacgctgc gaaccagtta ctaccggcgc gaggatggcg atgtgcgcca
     7921 ctacgtgtcg ttcaagctcg atccgcatcg cattccggga cttcccgagc cgcgcccgaa
     7981 gttcgagatc ttcgtgtttt cgccgcgttt cgaaggcgtg catctgcgcg gcggcagcat
     8041 tgcccgcggt gggctccgct ggtccgatcg tcttgaggac tatcgcaccg aggttctcgg
     8101 cctggtcaag gcgcagctcg ttaagaacgc cgtgatcgtt cccaccgggt cgaagggcgg
     8161 tttcgtcgtc aagcgcccgc cggtcggcgg cgaccgcgag gcgctgctcg ccgaaggtgt
     8221 ctcctgttac aagacctatc tcaaaggtct gctcgacgtc acagacaatc tcatcgatgg
     8281 gaaagtcgtg ccaccgccgg atgtcgtgcg ccgcgatggc gacgacccct acctggtcgt
     8341 tgctgccgac aagggcaccg cgaccttctc cgacatcgcc aacggcgtcg ccgccgaata
     8401 cggtttttgg ctcggcgacg cgttcgcctc cggcggctcg gtcggctacg accacaagaa
     8461 gatgggcatc accgcgcgcg gcgcctggga gtcggtcaag caccacttcc ggcaactcgg
     8521 cgtcgacacg cagagcgagg acttcacggt tgccggcgtc ggcgacatgt cgggcgacgt
     8581 attcggcaac ggcatgctgc tgtccaagca catccgcctc gtcgccgcct tcgaccaccg
     8641 gcacatcttt atcgacccgg acccggatgc ggcgaccagc tacgccgagc gcgagcgatt
     8701 gttcgccttg ccgcgctcgt cttgggagga ctacgacaac aagctgatct ccaagggcgg
     8761 cggtgtttgg ccgcgcaaga tcaagtcgat tccgctgtcg cctgaggttc gtcgcgtgtt
     8821 gggtgtcgaa gccaagacgc tgtcgtcgcc cgagctgatt tcggcgatcc tgcgtgcgcc
     8881 ggtcgatctc ttctacaacg gcggtatcgg cacctacgtc aaggccagcg acgagtcgca
     8941 cgcccaggtc ggtgatcgcg ccaatgacgg actgcgtgtc gacgccaccg aattgcgctg
     9001 tcgcgtcgtc gccgaaggcg gcaacctcgg cctgacgcaa cgcgcgcgca tcgagtttgc
     9061 agcgcacggc gggcgcatct acaccgacgc gatcgacaac tcggccggcg tcgattgctc
     9121 ggaccacgag gtcaacatca agattctgct cggcggcata gtcgctgcag gagacatgac
     9181 gctcaagcag cgcgaccgcc tgctggagga gatgaccgat gaggtggccg cactcgtact
     9241 ccaggacaac atccgacaga ctcaggtcct gagcgcgatg gcggcgcgcg caccgcagct
     9301 tttcggcgag caggtgcgct acatgcgcga acttgagcgt gccggccggc tgatccgccg
     9361 catcgagtac ctgccgaacg acgagaagat cgacgagcgc cggcaggccg gcgaagggct
     9421 gtggctgccc gagctgtcgg tattgctcgc ctacagcaag atggacctat acgacggcac
     9481 gctggcgagc aatgcgccgg atgacacggt gatcgccgag gcgctaacgt cctattttcc
     9541 gaagccgctg cagaagcgct tcgcgacgca gatcgcggct cacccgttgc gccgcgagat
     9601 catcaccacc gtcgtcacca acgaattggt caaccgcgtc ggctgtacct tcgcctatcg
     9661 gctgacgcag gagactggtg ccgacaccgg cgcaatcgtt cgggcttatg tgcttgcccg
     9721 cgaggtcttc ggcctcggcg cgctctggca ccaactcgac gcgcttgagc ggctgcccgc
     9781 agacgcgcag cgctcgatcg gcggcgcgct ggcgcggcag gccgagcgcg cggcgctatg
     9841 gttcctgcgg cggccagcgg tgctcgaaga cctagccgct tcggtcaaac gcttccggcc
     9901 ggtggtcacc gcgatcgatg cggcgcttga caaaggcctg cagccggtcg agtacgccgc
     9961 cttcgacgca gcgatcaagg cgcgtaccga cgccggtgtg ccggccgatg ttgcacgggc
    10021 cgtggcggca ctggtgccgc tgcacgcggc gctcgacatc accgaggtcg tctccgagac
    10081 cggacagaac gctgtggccg tcgccggcgc ctatttcgat ctggtcggcc ggctcgacct
    10141 ttactggctg agcagccgta tcctcgaact gccggcgtcg tcccgttggg aggagcgtgc
    10201 ccgcagcgcg ctgcgcgacg atctggaagc cgtggtcgcg acgctgaccg cgtggatctt
    10261 tcgccagtgc gagatcggca ataagggttg cgacgccaag gcgctgctcg acagctggca
    10321 gcagacgcgg tctacgcagc ttggccgcta tcaacagatg ctcggcgagc tgaagcaggg
    10381 gacgccgatc gactgggcga tgatttcggt gctattgcgc gaactgaagg cgctgtctcg
    10441 catctgagca ggcgcgtcgg cgataatcgc cgcttccgta acaggaggcg gcgatgcgct
    10501 actggctgat gaaatccgag ccttcggagg tatcgatcga cgacctcgcg aagatgccga
    10561 agaagaccgt tccctggttc ggtgtgcgca actatcaggc gcgaaacttc atgatgaacg
    10621 agatggccgt cggtgatggc gtgctgttct atcactcgag ctgcccgcag cccggcattg
    10681 ccggcatctg cgaagtcgcc tcgaccgcct atcccgacac gacgcagttt gacgccggga
    10741 gcccgtacca cgaccccaag gccagcgagg aaaagccgcg ctggtacaac gtcgatgtgc
    10801 aactcgtgag gaagatcccg ctgatcccac tcgccgccct tcgtgaggaa aaggcgctcg
    10861 ccgagatgct gattctcaag cgcggcaacc gactgtcgat caccccgctc acttccgccg
    10921 agtggcggca catcaccggc aagatggtgg ccaaatgacc tggttgttcc tctacctcgt
    10981 gctggggctg gtctcgggtt tcttcgccgg cctgctcggc atcggcggcg gcctgatgat
    11041 ggtgccggta ctgaccatcg cctttgccgc gcagggcttc cccgccgacg agctgctgca
    11101 cctcgcgctc ggcacctcga tggcaacgat cctgttcacc gccatcgcca gtctgcgtgc
    11161 gcaccacgcg cacggcgcgg tgatgtggcc ggtggtcaag tccttgacgc cgggcatcct
    11221 gctcgggacg ctgctcggca ccgcgattgc cggcgccgtg ccgacgcggc cgctgtcgat
    11281 cttcttcacc gccttcgtct gcctcaccgc ctggcagatg gcgaccggat tcaagcccaa
    11341 gccgcaccgc gaagtaccgg gtaccggcgg gctgatcggc gttggctcgg tcatcggcgc
    11401 cgtttcggcg ctggtggcaa tcggcggcgg cgcactgacc gtccccttcc tcacctggtg
    11461 caacgtccgt gtccagcacg cgatcggcac ctcggccgcg gtcggcctgc cgatcgccat
    11521 cggcggcacg attggctacg tcttcaatgg gtggtcacac cagttgcccg agtggagcgc
    11581 cggcttcgtc tacctgccgg ccgtcgccgc gctgatcgtc ggcagcatga gtaccgcccc
    11641 gctcggcgca aagaccgcac accggctgcc ggtgccgacc ttgaagcgaa tctttgccgg
    11701 gttattgctg gtgctggcgg gcaaaatgct ctggaacctc tttgcctagc cgaagaacat
    11761 cgtcttattc gccagagaaa cgcggacctc gcttctcgcg aaatgctgcc aatccttctc
    11821 ggtagtcggc ggtattaagc gtcccaagac tttcggcgca ttcctcgacg cttgggctgt
    11881 ggccgtcggc gaggcgaccc agccagtgct tgtgcgcgcg ggcaacgagc ggagcgccgg
    11941 cggcgattcg ctcggcggtg gcgcggattt catcggtcag cgcgtcggcg gcaaccacgc
    12001 gggtcagcag tcccttggcg agcgcttcgt cagcggcgag caggcgccct tcaaacagca
    12061 gttcagcgag aaccggccgc ggcacggtgc gcagcaccag cgccatttcg ccggcgtaca
    12121 tcgagaagcc caggcgattg atcggcacgc cgaagcgcgc gttcggggcg gcgatgcgca
    12181 gatcgcagca gcaggcgatt tccagtccgc cgccgacgca ggcgccgcgg atctcggcga
    12241 tggtcggcac cggaaagtcg gctagcgcct gcagcgccgg ggcgacggcc tcggcgtgat
    12301 aggcgagtgc cgcctcgggt gttgcgcgcg cctcgtcgaa ttcgtcgatg tcgc
//
LOCUS       DATDAA010000462         5640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_277954,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000462 DATDAA010000000
VERSION     DATDAA010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277954"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..1304)
                     /gene="gap"
                     /locus_tag="VIS73_14140"
     CDS             complement(306..1304)
                     /gene="gap"
                     /locus_tag="VIS73_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY5764345.1"
                     /translation="MAIKVGINGFGRIGRMVFRAAIRDFSDIEIVGINDLLDPDYLAY
                     MLQYDSVHGRFKGEVKVEGNTLVVNGKKIRLSAEKDPANLKWNEVGADIVVESTGLFL
                     DKAGAEAHLKAGAKKVIMSAPSKDDTPMFVYGVNHTSYKGEAIVSNASCTTNCLAPVA
                     KVLNDSFGIKRGLMTTVHAATATQKTVDGPSKKDWRGGRGILENIIPSSTGAAKAVGK
                     VIPELNKKLTGMSFRVPTSDVSVVDLTVELNKEASYEQICAAMKAAADGPMKGVLKYT
                     TDKVVATDFRGETCTSVFDADAGIALDPTFVKVVAWYDNEWGYSCKVLEMVRVIAK"
     gene            complement(1320..3338)
                     /gene="tkt"
                     /locus_tag="VIS73_14145"
     CDS             complement(1320..3338)
                     /gene="tkt"
                     /locus_tag="VIS73_14145"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY5764346.1"
                     /translation="MQTRAADVLRSPVTGAIRALAMDAVQKANSGHPGMPMGMAEIAD
                     VLWRRHLRHNPANPVWADRDRFVLSNGHGSMLLYALLHLTGYDLPIDQLKQFRQLHSK
                     TPGHPEYGYAPGVETTTGPLGQGISNAVGMAMAEKLLAAEFNRPGHEIVDHHTYVFLG
                     DGCLMEGISHETCALAGTWGLSKLIAFWDDNGISIDGHVEGWFTDDTPKRFAAYGWNV
                     IAGVDGHNTEAIDAAIKQAKAQNDKPTLICCKTTIGLGSPNKAGTHDVHGAALGDAEV
                     AATREAIGWPHAPFEIPADVYAAWDAKKKGQEFEAGWQARFDAYAKDFPELAAEFTRR
                     MAGELPKNWKAHAQAAIDAVNVKGETVATRKASQIAINALVPVLPEFVGGSADLTGSN
                     LTNWKDCTHVSGKSVGNYISYGVREFGMAAIMNGMALHKGVLPFGGTFLMFSEYARNA
                     LRMAALMKQRAIYVFTHDSVGLGEDGPTHQPVEQTATLRMIPNMDVWRPCDTVETMVA
                     WVAAVEKTTGPTSLCLSRQNLPFVKRDAATIASVSRGGYVLSEAKGAPRAVIIATGSE
                     VDIALKAQAALAADGIAVRVVSMPATTVFDRQDAAYKSQVLPAGVPRVAVEAGVSDGW
                     YKYVGAADGKQGAVLGLDRFGESAPAGQLFKEFGFTSENLTHLVKSVI"
     gene            complement(3567..4064)
                     /locus_tag="VIS73_14150"
     CDS             complement(3567..4064)
                     /locus_tag="VIS73_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764347.1"
                     /translation="MKISSRSTHKLALATLVTALAASGALAQHAGHGSGQRMGGMGGK
                     GMHAGGHAMGPGAVMEMCDGKDSMPPRYCAPHYHVMSSVPGAAIGGAEPMGDKALMVT
                     LKRLPGAANPALVIVGGGGDLAGATTVAAGWKDGAMVHVDLTGEGSLYRQRGVHLHLF
                     PLTGK"
     gene            complement(4144..4923)
                     /gene="scpB"
                     /locus_tag="VIS73_14155"
     CDS             complement(4144..4923)
                     /gene="scpB"
                     /locus_tag="VIS73_14155"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF008506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HEY5764348.1"
                     /translation="MPFIAIEQTATVATITLDNPKKLNALSETLVQETIAALKDFAAA
                     GIRVAILRARPGVKVWSAGHDVAELPEGRRDPLGWSDPLRELIRTIETFPAPVIAMIE
                     GGVWGGAVETVMACDLVIAAPSATFAVTPARLGVPYNVSGMMTFMSAASIRIVKEMAF
                     TAKPIGAERAERIGMINHLVAAEELEAFTAEMAATIADNAPLSIMAMKEELRVLAGAK
                     PVSPRGFEKMQGLRRQVYDSHDYAEGIRAFKEKRRPVFLGR"
     gene            complement(5064..>5640)
                     /locus_tag="VIS73_14160"
     CDS             complement(5064..>5640)
                     /locus_tag="VIS73_14160"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEY5764349.1"
                     /translation="TSPEAFLQPGTKQVAAGYAVYGPTTLLVLTLGRGVNGFTLDREH
                     GRFELTQPNIRIPEDTAEFAINMSNMRHWEAPVKRYVDEMLAGKNGPLGKDYNMRWVA
                     SMVADVHRIMTRGGIFMYPLDAKTREKGGKLRLMYEANPMALLVEQAGGAATTGRQRL
                     LDLQPTGLHQRVPVILGSKHEVERVTAYHRD"
BASE COUNT          867 a         1765 c         1936 g         1072 t
ORIGIN      
        1 cgttgtagag cttgccgccg gcgcctccgc cgttcgtggc tcgtcggctt tggcggcagg
       61 gacgggcgct tcggccggct gtgccggcgc cactcggtcg gccgccggcg cggcgggcgg
      121 cgcttgctgc aagagcgggg cgacgccgca ggcgccgagc gtcagcgcca gcgccatgga
      181 aaaacccgcg ccggcagggc gccgggcgcg ggttgtgcgc gagtatccag tcaccgtgcc
      241 gccggccctg agacggcagt ccgcggtcgg tgggggcgac attggtggcc gaatttcgca
      301 gcagcttatt tggcgatgac gcgaaccatc tccagcacct tgcacgagta accccactcg
      361 ttgtcgtacc aggcgacgac cttgacgaag gtgggatcga gcgcgatgcc ggcgtcggcg
      421 tcgaagaccg aggtgcaggt ctcgccgcgg aagtcggtgg cgacgacctt gtcggtggtg
      481 tacttgagca cgcccttcat cgggccgtcg gcggcggcct tcatcgctgc gcagatctgc
      541 tcgtagctgg cttccttgtt cagttcgacg gtcaggtcga cgaccgagac gtccgaggtt
      601 gggacgcgga agctcatgcc ggtcagcttc ttgttcagct cggggatcac cttgccgacg
      661 gccttggcgg cgccggtcga gctggggatg atgttctcga ggatgccgcg accaccgcgc
      721 cagtccttct tcgacgggcc gtcgacggtc ttctgcgtcg cggtggcggc atggaccgtg
      781 gtcatcaggc cgcgcttgat gccgaaggaa tcgttcaaca ccttggccac cggtgccaga
      841 cagttggtcg tgcagctggc attggagacg atcgcctcgc ccttgtacga cgtgtggttc
      901 acgccgtaga cgaacatcgg cgtgtcgtcc ttcgacgggg ccgacatgat gaccttcttc
      961 gcgccggcct tgaggtgcgc ttcggcaccg gccttgtcga gaaagaggcc ggtcgattcg
     1021 acgacgatgt cggcgccgac ttcgttccac ttcaggttgg ccgggtcctt ctcggcggag
     1081 agacggatct tcttgccatt gaccaccagc gtgttgccct cgactttgac ttcgcccttg
     1141 aagcgaccgt ggaccgagtc gtactgcagc atgtaggcga ggtagtcggg atcgagcagg
     1201 tcgttgatgc cgacgatctc gatgtccgag aaatcacgga ttgccgcgcg gaagaccatg
     1261 cgaccgatac gcccgaagcc gttgatgcca accttgatag ccatgaagtg ttctcctggt
     1321 tagatgaccg atttgacgag atgggtaaga ttttctgacg tgaagccgaa ttccttgaag
     1381 agctgaccgg cgggtgccga ctcgccgaag cggtcgaggc cgagcacggc gccctgcttg
     1441 ccgtcggcgg cgccgacgta cttgtaccag ccatcgctga cgccggcttc gacggcgacg
     1501 cgcggcacgc cggccggcag cacctgcgac ttgtaggccg cgtcctggcg atcgaagacc
     1561 gtggtcgcag gcatcgacac gacgcgcacg gcgatgccgt cggcggcgag cgctgcctgc
     1621 gccttaagcg cgatgtcgac ctccgagccg gtggcgatga tcaccgcgcg cggtgcgccc
     1681 ttcgcttccg atagcacata accgccgcgg gaaacggatg cgatggtcgc tgcatcgcgc
     1741 ttgacgaagg gcaggttctg ccgcgacagg cagagcgagg tcgggccggt ggttttttcg
     1801 actgccgcaa cccacgcgac catcgtctcg accgtgtcgc agggacgcca gacatccatg
     1861 ttcggaatca tgcgcagcgt cgcggtctgt tcgaccggct ggtgcgtcgg gccgtcctcg
     1921 ccgaggccga ccgagtcgtg cgtgaagacg tagatggcgc gttgcttcat cagcgccgcc
     1981 atgcgcaggg cgttacgggc gtactcggag aacatcagga aggtaccgcc gaagggcagt
     2041 acgcccttgt gcagcgccat gccgttcatg atcgcggcca taccgaactc gcgcacgccg
     2101 taggaaatgt agttgccgac cgacttgccg gagacgtgtg tgcagtcctt ccagttggtc
     2161 aggttagagc cggtgaggtc ggccgacccg ccgacgaatt cgggcagcac gggcaccagc
     2221 gcgttgatgg cgatctgcga ggccttgcgc gtggcgacgg tctcgccctt tacgttgact
     2281 gcgtcgattg ctgcctgcgc gtgggccttc cagttcttcg gtaactcgcc ggccatacgc
     2341 cgcgtgaact cggcggcgag ttccgggaag tccttggcat aggcgtcgaa gcgcgcctgc
     2401 cagcccgcct cgaattcctg acccttcttc ttcgcgtccc aggctgcgta gacatcggcg
     2461 ggaatctcaa agggcgcgtg cggccagccg atggcttcgc gcgtcgccgc gacttcggcg
     2521 tcgccgagtg ccgcaccgtg tacgtcatgg gtgcctgcct tgttcggcga gccaaggccg
     2581 atggtggtct tgcagcagat cagcgtcggt ttgtcgtttt gtgccttggc ctgcttgatc
     2641 gccgcgtcga tcgcctcggt gttgtggccg tcgaccccgg caatgacgtt ccagccgtag
     2701 gcggcgaaac gcttcggggt gtcgtcggta aaccagccct cgacatggcc gtcaatcgag
     2761 atgccgttgt cgtcccagaa ggcgatcagc ttggacagcc cccaggtccc ggcgagcgcg
     2821 caggtctcgt gcgagatgcc ttccatcaga cagccgtcgc cgaggaagac gtaggtgtgg
     2881 tgatcgacaa tctcgtggcc gggccgattg aattcggcag ccagcagctt ctcggccatc
     2941 gccatgccga ccgcgttgct gatgccctga ccgagcggcc cggtggtggt ttcgacgccc
     3001 ggagcgtagc cgtactcggg gtggcccggc gtcttcgagt gcagctggcg gaactgcttc
     3061 agttggtcga tcggcaggtc gtagccggtg aggtgcagca gcgcgtagag cagcattgag
     3121 ccgtggccgt tcgagagcac gaagcggtcg cggtcagccc agaccgggtt ggccgggttg
     3181 tggcgcaggt gacggcgcca gagcacgtcg gcaatctcgg ccatgcccat cggcatgccc
     3241 gggtggccgg aattggcttt ttgcaccgcg tccatcgcca gcgcgcggat ggcaccggta
     3301 acgggcgaac gaaggacgtc ggcagcgcga gtctgcatgc tgttctccta cagagagaat
     3361 cctgctgatt attgttgtgc gccgggctgc cagatcaatc ggaaatcggc gtggcaatca
     3421 cggcgggaag gccgcgatta tgcggaaaag cggcgcgctt gaaaagccgc gccgcgtcat
     3481 tccgaaggca tattacgggt taacctgaag ggattatgct ggccgtatcc gtttggctag
     3541 ccctcggaat aacccgcttg gcgcggctat tttcctgtca gcgggaagag gtgcaggtgc
     3601 acgccgcgct ggcggtagag cgagccctcg ccggtgagat cgacatggac catggcgccg
     3661 tccttccagc cggccgcgac ggtggttgct ccggccaggt cgccaccgcc gccgacaatg
     3721 accagcgccg ggttcgcggc accgggcagg cgcttgagcg tcaccatcag cgccttgtcg
     3781 cccatcggtt cggcaccgcc gatcgccgcg ccgggcaccg aactcatcac gtggtagtgc
     3841 ggtgcgcaat agcggggcgg catgctgtcc ttgccgtcgc acatctccat gactgcgccg
     3901 gggcccatcg cgtggccgcc ggcatgcatt cctttgccgc ccatcccccc cattctctgt
     3961 ccgctgccgt ggccggcgtg ctgtgccagc gcgccactgg cagccagcgc ggtcaccaac
     4021 gtggcgagcg cgagcttgtg ggttgatcgg cttgagatct tcatcgtcgt tgctccgctg
     4081 tgagaaaggt cgcaacgatt ataggccgct gatgcaatcg gcatcacagg gcccgctcgc
     4141 cgatcagcgg ccgagaaaca ccgggcggcg cttttccttg aaggcgcgga taccttcggc
     4201 gtagtcgtgg ctatcgtaga cttggcggcg caggccctgc atcttctcga agccacgagg
     4261 gctgaccggc ttggcgccgg cgagtacgcg cagttcttcc ttcatcgcca tgatcgacag
     4321 cggcgcgtta tcggcgatcg tcgccgccat ctcggcggtg aaggcttcga gttcctccgc
     4381 agcgacgagg tgattgatca tgccgatgcg ctcggcccgc tcggcgccga tcggcttggc
     4441 ggtaaaggcc atctccttga cgatgcggat gctcgcggca ctcatgaagg tcatcatgcc
     4501 gctgacgttg tatggcacgc cgaggcgcgc cggggtgacg gcgaatgtgg cgctcggcgc
     4561 ggcgatgacg aggtcgcagg ccatcaccgt ctcgacggcg ccaccccaga cgccaccctc
     4621 gatcatcgcg atgaccggtg ccggaaaggt ctcgatggtg cggatcagtt cgcgcaaggg
     4681 atcgctccag cccagcgggt ctcgccggcc ctcgggcagt tcggcaacat cgtgcccggc
     4741 cgaccagacc ttgacgccgg gacgggcgcg caggatggcg acgcgaatgc ccgcggcggc
     4801 gaagtccttt agcgcagcaa tcgtttcttg gaccagtgtt tcgctcagcg cgttgagctt
     4861 tttcggattg tcgagcgtga ttgttgcgac cgtcgcggtc tgctcgatgg cgatgaaggg
     4921 catggcgatt cctcgttggc ggccgcgcag cggcggcggg ttcgtcggcg gaccattaga
     4981 tgccttatcg tcgttcgcgc ccattgcgga tttccgcaac ctgcggaaga ccacaacgcg
     5041 gggggcgccc cgccgccggt cgatcagtcg cggtggtagg cggttacccg ctcgacctcg
     5101 tgcttggaac cgaggatcac cggcacgcgc tggtgcaggc cggtcggttg caggtcaagg
     5161 aggcgctggc ggccggtggt ggctgcgccg ccggcctgtt cgaccaggag tgccatcgga
     5221 ttggcctcgt acatcagcct cagcttgccg cccttttccc gggtcttggc gtcgagcggg
     5281 tacatgaaga tgcccccgcg ggtcatgatg cggtgcacgt cggcgaccat cgaggcgacc
     5341 cagcgcatgt tgtagtcctt gccgagcggg ccattcttgc ccgcaagcat ctcgtcgacg
     5401 tagcgcttca ccggtgcttc ccagtggcgc atgttcgaca tgttgatggc gaactcggcc
     5461 gtgtcttccg gaatgcggat gttcggctgg gtcagttcga agcggccgtg ctcgcggtcg
     5521 agcgtgaagc cgttgacgcc gcggccgagt gtcagcacca gtagcgtcgt cggcccatag
     5581 acggcgtaac cagcggcgac ctgtttggtg cccggctgca ggaaggcttc gggcgaggtc
//
LOCUS       DATDAA010000463         4295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_278031,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000463 DATDAA010000000
VERSION     DATDAA010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278031"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="VIS73_14165"
     CDS             complement(<1..164)
                     /locus_tag="VIS73_14165"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEY5764350.1"
                     /translation="MEMKRLNAGRLRAAGYDARSRILRVELDDGSAIDYSGVGEELWR
                     RLASSGSAWS"
     gene            complement(226..783)
                     /locus_tag="VIS73_14170"
     CDS             complement(226..783)
                     /locus_tag="VIS73_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY5764351.1"
                     /translation="MTIGPEGFVGDQQADRRVHGGPDKAVHLYPAAHYARLAKRFLKA
                     AAQFVPGSIGENISTAELTEADVRVGDIWQLGSARLQVCQPRNPCWKIDARYGCEGIA
                     RFIASESIAGWYWRVLAPGIVDGGDTLELVEPARAPTLREALSLLGEHRPSLDRLQQL
                     IDAPGIAENWRTVLVKRFDWLHRNT"
     gene            complement(909..1427)
                     /locus_tag="VIS73_14175"
     CDS             complement(909..1427)
                     /locus_tag="VIS73_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764352.1"
                     /translation="MDILAHGLWTGAALKATARRNTIARRTALWTIALSVLPDFGHAL
                     PVAIWSLFAGAPALFLDYALASPGSEPPMPEVVALWSHHLHCTLHSGLVAALVSLMVA
                     LAARRFWLPLAGWWSHIVIDVFTHSNSFYPVPVFYPLSYWGFDGLAWNSPAFMVTNYL
                     ALALVWTLLLRR"
     gene            complement(1587..2420)
                     /locus_tag="VIS73_14180"
     CDS             complement(1587..2420)
                     /locus_tag="VIS73_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764353.1"
                     /translation="MTRKDLLVIALLAFGVALPATANQAYFENYRKQLAQEIEMLDWN
                     LRHSSVAPSLAPRTQQDSLDKIERAKEALGSGNTRLAEDLAAEAALPLTTVARKDDLG
                     SHPDQLRYFREIRQALLSITDSAERISDEQGAARDYRSDTLVALAQAEYELKAGNVEA
                     AFAIIGDRYDAVSQSAADYRRGQTFTIAATEWRDGKQFQDGVRRIDERRALTEYLILE
                     AQAEGIDQTPLREALAIAKASLAQATGYAQQEHWDKAYASLELAFAEIEDRWRMVGLE
                     W"
     gene            complement(3092..3499)
                     /locus_tag="VIS73_14185"
     CDS             complement(3092..3499)
                     /locus_tag="VIS73_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY5764354.1"
                     /translation="MALQLQIARLLSCALIGTLPALATAQGWQPPPKPSPGLMPNPSA
                     GKALFASHCAVCHGADLRGSDRGEKRGPPLLHKIYEPSHHSDAAFQMAVRDGTRSHHW
                     NFGDMPPVPGVTPDDVAHITAYVRAEQRRAGIR"
     gene            complement(3561..>4295)
                     /locus_tag="VIS73_14190"
     CDS             complement(3561..>4295)
                     /locus_tag="VIS73_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY5764355.1"
                     /translation="LLAAYIYVDVRERDIGSYVKDAQQAVAEKVQFPPGYYATWSGQF
                     EYMERAKAKLAIVVPFTLAIIFVLLFLNFRRLTETLIVMLSVPFALVGGIWLMWWLGY
                     NMSVAVAVGFIALAGVAAETGVVMLIYLDHAWKEVRARCEEEGRQPSASDLYAAIMEG
                     AVERVRPKMMTVVAIMAGLLPIMWSTGTGSEVMSRIAAPMVGGMISSTVLTLGVIPAL
                     YALVKEWQLKRRLRGEPSIVPIAQAR"
BASE COUNT          775 a         1304 c         1435 g          781 t
ORIGIN      
        1 aaactccacg cagaacccga actggccaag cgtcgccaga gctcctcgcc gacgcctgaa
       61 taatcgatgg cgctgccatc gtccagttca acgcgcaaga tccgcgaccg ggcgtcgtat
      121 cccgccgcgc gtagtcggcc ggcattgagt cgtttcatct ccatcgttgc ctcgtgttca
      181 ggatgcggtg gactaaacgg ccattatgac tcgaccgcct gatgatcagg tgttgcggtg
      241 cagccagtcg aatcgtttga caagcacggt tcgccagttt tccgcgattc ccggtgcgtc
      301 gatcaattgc tgaaggcggt cgagcgatgg ccggtgttcc ccgagaaggc tcaaggcctc
      361 gcgcagcgtt ggcgcacgcg cgggttcgac cagttcgagc gtgtcgccac cgtcgacgat
      421 ccccggcgcc aggacgcgcc agtaccaccc ggcgatcgat tcgctggcga tgaatctggc
      481 gataccttcg cagccgtaac gcgcgtcgat cttccagcag ggattgcgcg gctggcagac
      541 ctgcaggcgc gcgctgccga gttgccagat atcgccgacg cgcacgtcgg cctcggtcag
      601 ctcggcggtc gagatgttct caccgatcga tccgggaacg aattgcgcag cggccttcag
      661 gaatcgtttc gcaaggcgag cgtagtgcgc ggccgggtac aggtggacgg ccttgtcggg
      721 gccgccgtgc acgcgccggt cggcctgctg atcaccgacg aatccctcgg gtccgatcgt
      781 cacgggaaac gcgaccggta acttgtagat gccgcttgga cggccgattt ccggcaatgg
      841 ccgaatacaa ccggaaaaca gggagacggt tacccgcatg aattccttaa tggatgtttg
      901 cgagacgatc agcgccgcaa caatagcgtc cagacgagcg cgagcgccag gtagttcgtg
      961 accatgaagg ccggcgaatt ccaggccagc ccgtcgaaac cccaatagct cagcgggtag
     1021 aaaaccggca ccgggtagaa gctgttcgaa tgcgtgaaga cgtcgatcac gatgtgcgac
     1081 caccagccgg cgagcggcaa ccagaagcgg cgggcagcca gcgcgaccat aagcgagacg
     1141 agcgcggcga caaggccgct gtgcagggtg cagtggaggt ggtgcgacca gagcgcgaca
     1201 acctcgggca tcggcggttc gctgccgggg gaggcgagtg cgtagtcgag gaacagcgcc
     1261 ggcgcgccgg cgaacagcga ccagatcgcg accggcagcg cgtggccgaa atcggggagt
     1321 accgagaggg cgatcgtcca cagcgctgtt cgccgcgcaa tggtgtttct ccgcgccgtg
     1381 gccttgaggg cggcgcctgt ccagagtccg tgtgcgagga tgtccacgcg ttcgcgttgc
     1441 ccgttgcgag aggagcaatc actctacgcc ggattgactc agaacagcgt ccgagtgctt
     1501 ccggggcggg tcgtcagatc cgccgcagca gacaaaccac gcagtctatt gtgtgcgtct
     1561 ctaaccggtt tatgcgaacc aacggcttac cactcgaggc cgaccatgcg ccagcggtcc
     1621 tcgatttcgg cgaaggccaa ttccagagag gcgtaggcct tgtcccaatg ttcctgttgg
     1681 gcgtagccgg ttgcctgggc gagcgaagcc ttggcgattg ccagcgcctc acgtagcggt
     1741 gtctggtcga tcccttctgc ctgcgcttcg agaatcaggt actcggtcag tgcacggcgc
     1801 tcgtcgatgc gtcgaacgcc gtcctgaaac tgctttccgt cgcgccactc ggtagcggca
     1861 atcgtgaagg tctggccgcg tcgatagtcg gctgcggatt ggctgacggc atcgtagcga
     1921 tcgccgatga tcgcgaacgc ggcctcgacg tttccggcct tcaattcgta ctcggcttgc
     1981 gcgagggcaa cgagcgtatc gctgcgatag tcgcgcgccg caccttgctc gtcagagatg
     2041 cgttcggcac tgtcagtgat cgacagcagc gcctgccgaa tctcgcggaa atagcgaagc
     2101 tggtctgggt gcgacccaag gtcgtccttc cgtgccaccg tcgtcaaggg cagcgcagct
     2161 tcggccgcca ggtcttcggc gagccgcgtg tttccgctgc ccagcgcttc cttcgcgcgc
     2221 tcgatcttgt ccagtgaatc ctgttgagtt ctgggtgcga gtgatggcgc aaccgacgag
     2281 tgccggagat tccagtcgag catctcgatt tcctgcgcca gctgtttgcg gtagttctca
     2341 aagtaggcct ggtttgcggt cgccgggagc gcaacgccaa aggccagaag tgcgatgacg
     2401 agaaggtcct tgcgggtcat gtcaattctc caagacgtgt gtgcgctgtg acggatcaca
     2461 ctgccgagaa gttccgggct ggtggctttc ggtgggtgag agtcgacgcg ttggcgcatc
     2521 ggtgcagcgt gctggtcacg gtgttcagcg tcgattcggg attggtgcag cgcgggacat
     2581 gactgatcgg cagtcgtgtc gactgtcgga aaaatttaca agtggccgtt acccggcctt
     2641 gtctcggtgg tgctatttgg agagaacgct atacgatagc gtgggggcgt aggtctttcc
     2701 gccacgtgct gtgtgacgaa actgtaatgt cccggtccca tccgtgaaag caagcaacgc
     2761 ggatgattgc cgtaggctac ctgcggagac ctacgatgcc agcacatgca gatacgagac
     2821 cggccgagcc tgcggctgga cgtccggcga accggtgcgc gacgactact ggtgaagccg
     2881 aagtcgtacc gtcgtcagcg ttgaaccagg aaccagaatc gccgtcgcag gggaaaccgg
     2941 gcgctcatgg cgccagtggc ttgatatccc gggtgcgggc ctggatggcc gagaacgaga
     3001 aaatgcacct ggtgagccgg acgaccccct gctgcaacgg gcgtcgctga ttcgagctca
     3061 cgccgacgac ggcgcggtgc gcctatagcg gctagcggat accggcgcgg cgctgttcag
     3121 cacggacgta ggcagtgata tgggcgacgt cgtcaggcgt gacgcccggc accggtggca
     3181 tgtcgccgaa attccagtgg tgcgaccggg tgccgtcgcg tacggccatc tggaaagccg
     3241 catccgagtg gtgcgatggc tcatagatct tgtgcagcag cggcggtccc cgcttctcac
     3301 ccctatccga gccgcgtagg tcggcgccat ggcagaccgc gcaatggcta gcaaacagcg
     3361 ccttgccggc ggacgggttg ggcatcagtc cggggctcgg ctttggcggt ggctgccagc
     3421 cctgcgctgt cgccagcgcg ggcaaggtgc cgatcagcgc gcaggacaac aggcgggcga
     3481 tctgtaattg gagggccatg cgagcggctc cttcgtgctg gatcgtttcg gtcggcgggc
     3541 gttcggcttg cactggtcgg tcagcgtgcc tgcgcaatcg gcacgattga cggctcgccg
     3601 cgcaatcgcc gcttcagctg ccattccttg accaaggcgt agagcgccgg gatcacgccg
     3661 agcgtgagca cggtcgagga gatcatgccg ccgaccatcg gtgcggcaat ccggctcatc
     3721 acctccgagc cggtgccggt gctccacatg atcggcagca ggccggccat gatcgcgacc
     3781 acggtcatca tcttcggccg cacacgctcg acggcgcctt ccatgattgc cgcgtagagg
     3841 tcgctggcgc tcggttgccg gccttcctcc tcacacctgg cgcggacttc cttccaggcg
     3901 tggtcgagat agatcagcat tactaccccg gtttcggcgg cgacgccggc cagcgcgatg
     3961 aagccgacgg cgacggcgac gctcatgttg taaccgagcc accacatcag ccagatgccg
     4021 ccgaccagcg cgaagggcac cgagagcatg acgatcagcg tttcggtcag gcgccggaag
     4081 ttcaggaaga gcagcacgaa gatgatcgcc agcgtgaagg gcacgacgat ggcgagcttg
     4141 gccttggcac gctccatgta ctcgaactgg ccgctccaag ttgcgtagta gccgggcgga
     4201 aactgcacct tctcggcgac ggcctgctgg gcatccttga cgtatgagcc gatgtcgcgc
     4261 tcgcgcacgt cgacgtagat gtaggcggcg agcag
//
LOCUS       DATDAA010000464         2056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_209405,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000464 DATDAA010000000
VERSION     DATDAA010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209405"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VIS73_14195"
     CDS             complement(<1..417)
                     /locus_tag="VIS73_14195"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEY5764356.1"
                     /translation="MITAAIVLAEAAVLYDGLNAVQTQVYGAAARGGATRSDVIISDS
                     QINFPKVIQPNVLVCLTQEAYSKFFPIIRPGGLLLTDSRYVKTERKVDARQVELPMYQ
                     AVMEKIGKPIVFNICMLGAVIGLTELVHDTSIMRVLE"
     gene            complement(500..1300)
                     /locus_tag="VIS73_14200"
     CDS             complement(500..1300)
                     /locus_tag="VIS73_14200"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY5764357.1"
                     /translation="MAVKDYLRERFLPHLWCAGCGHGTVLNGLVRAIETLGMSKNEIV
                     MVSGIGCSSRISGYTDFHTLHTIHGRALAFATGVKLSKPELNVIVPMGDGDALAIGGN
                     HFIHAARRNIDITAIVMNNRVYGMTGGQFSPLSNAGTLATTAPYTNIDQNFDVAKLAE
                     AAGATFVARTTVYHVQQMSDLIRQAIVHEGFSVVEILTQCPTYYGRRNKEGGPVEMMK
                     LFKDRTTPIGSKAKQADPSLVERGIFVQKEMPEYCSQYDAIIEKATRR"
     gene            complement(1304..>2056)
                     /locus_tag="VIS73_14205"
     CDS             complement(1304..>2056)
                     /locus_tag="VIS73_14205"
                     /inference="COORDINATES: protein motif:HMM:NF028456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764358.1"
                     /translation="GDHAIVAMTASNHQDVFEVTVDAFNVAETYRTPVILLFDEVVGH
                     MRERLVIPQPGELPLVDRLRTAVKEGVDYHPYLPREDGRLPMSDFGGVHRYNVTGLFH
                     DMWGFPSDNPKIVHGLLRHLVDKIENNVNQIARYKELYLEDADYVLVSYGSAARSARH
                     VVESLRPRGERIGLLELQTLWPFPSDIVREKCARAKYIFVVELNMGQIRQEVQTAVDH
                     PERVFLVNRIDGVFITPTDIRNILRLIMGKGV"
BASE COUNT          332 a          649 c          677 g          398 t
ORIGIN      
        1 ttcgagcacc cgcatgatcg aggtgtcgtg gaccagttcc gtcagcccga tcacggcacc
       61 cagcatgcag atgttgaaaa cgatcggttt gccgatcttt tccatcaccg cctggtacat
      121 gggcagttcg acctggcgcg cgtcgacctt gcgctcggtt ttcacgtagc ggctgtcggt
      181 cagcagcagg ccgccggggc ggatgatcgg gaaaaatttg ctgtaggctt cctgggtcag
      241 gcagaccagc acgttgggct ggatcacctt ggggaagttg atctggctgt ccgaaatgat
      301 cacgtccgaa cgggtcgccc cgccgcgcgc ggccgccccg tagacctggg tctgcacggc
      361 gttgaggccg tcgtagagca cggccgcctc ggccagcacg atggcggcgg tgatcacgcc
      421 ctgcccgccc gaacccgaaa acaccattct gcatcgttcc attgtatttc cttccggccg
      481 gcgcccggcc ctcgcgcccc tagcgccggg tcgccttttc gataatcgcg tcgtactggc
      541 tgcagtactc gggcatttcc ttctgcacga agatgccgcg ctcgaccagg ctcgggtcgg
      601 cctgcttggc cttggagccg atcggcgtgg tgcgatcctt gaacagtttc atcatttcga
      661 ccgggccgcc ttccttgttg cggcggccgt agtaggtcgg gcactgcgtc aggatctcca
      721 ccacggaaaa cccttcgtgc acgatcgcct ggcggatcag gtcgctcatc tgctgcacgt
      781 gatagaccgt cgtgcgcgcc acgaaggtcg cgccggcggc ttcggccagc ttggcgacgt
      841 cgaagttctg atcgatgttg gtgtacggcg ccgtggtcgc cagggtcccc gcgttggaca
      901 gcggcgaaaa ctgcccgccg gtcatgccgt agacccggtt gttcatcacg atcgccgtga
      961 tgtcgatgtt gcgccgcgcc gcgtggatga agtggttgcc gccgatggcc agcgcatcgc
     1021 cgtcgcccat ggggacgatc acgttcagtt ccggcttgct cagtttgacg ccggtggcga
     1081 acgccagcgc gcggccgtgg atggtgtgca gggtgtgaaa gtccgtgtag ccggagatgc
     1141 gcgaggagca cccgatgccc gagaccatca cgatttcgtt tttgctcatg ccgagggtct
     1201 cgatggcgcg gaccaggccg ttgagcaccg tcccgtggcc gcagccggcg caccacaggt
     1261 gcggcagaaa acgttcgcgc aggtagtctt tgaccgccat agtttaaacc cccttcccca
     1321 tgatcaggcg cagaatgttg cgaatgtcgg tgggtgtgat gaaaacgccg tcgatgcggt
     1381 tgaccaaaaa gacccgctcg gggtggtcga cggccgtctg gacctcctgg cggatctgcc
     1441 ccatgttcag ctcgaccacg aaaatgtact tggcgcgggc gcacttttcg cgcacgatgt
     1501 cgctcggaaa gggccacagg gtctgcagtt cgagcaggcc gatgcgctcg ccgcgcggcc
     1561 gcaggctctc gaccacgtgc cgggccgaac gggccgccga cccgtaggag accagcacgt
     1621 aatcggcgtc ctccagatag agctccttgt agcgcgcgat ctggttgacg ttgttctcga
     1681 tcttgtcgac caggtgccgc agcagcccgt gcacgatctt ggggttgtcc gaggggaagc
     1741 cccacatgtc gtggaacagg ccggtcacgt tgtagcggtg caccccgccg aaatcggaca
     1801 tcggcaggcg accgtcctcg cgcggcaggt aggggtggta gtcgaccccc tccttgaccg
     1861 ccgtgcgcag gcggtcgacc agcggcagct cgccgggctg gggaatcacc aggcgctcgc
     1921 gcatgtgccc cacgacctcg tcgaacaaca ggatgaccgg ggtgcggtag gtttcggcca
     1981 cgttgaaggc gtcgaccgtc acttcgaaga cgtcctggtg gttggaggcc gtcatggcca
     2041 cgatggcgtg gtcccc
//
LOCUS       DATDAA010000465         3249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_314305,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000465 DATDAA010000000
VERSION     DATDAA010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314305"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..900)
                     /locus_tag="VIS73_14210"
     CDS             complement(16..900)
                     /locus_tag="VIS73_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5764359.1"
                     /translation="MPPSTATLVRFAPLLFVFLWSTGFIGAKYGLPHAEPLSFLLVRY
                     LLVLALMTMLALATRAPWPRDPHQWLHIGVSGLLVHATYLGGVFIAIDHGLPAGVTSL
                     LVGLQPILTALGAGWLLRDAVSGRQWLGLWLGLAGTALVVGADLALDRPLAELLPMLL
                     PALAALLGITAGTLYQKRFCAHFDLRTGAVIQFLPTAIATGIGVALFESLAIDWNGEF
                     VFALAWLVGVLSLGAISLLNLMIRQGSAVHVASLFFLTPPTTALIAFLIFGETLSLPA
                     VAGMIIAVVGVRLARKAA"
     gene            complement(962..1690)
                     /locus_tag="VIS73_14215"
     CDS             complement(962..1690)
                     /locus_tag="VIS73_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5764360.1"
                     /translation="MAGHSKWANIQHRKGRQDAKRGKVFTKLIKEITVAAKMGGGDPN
                     MNPRLRLAIDKAKGESMPKDNIENAIKRGTGQLEGVSYEEIRYEGYGIGGAAVMVDCL
                     TDNKTRTVAEVRHAFAKHGGNMGTEGSVSFQFKHCGQLIFAPGTDEAKLMDAAIEAGA
                     EDVAINEDGSIEVLTPPTGEFASVKEAVEAAGFTAEFAEVTMKPLNETPLTGEDAEKM
                     QKLLDAIDDLDDVQEVYTTADIDE"
     gene            1890..>3249
                     /locus_tag="VIS73_14220"
     CDS             1890..>3249
                     /locus_tag="VIS73_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEY5764361.1"
                     /translation="MSDPIVIARQGDREIALLPQFANRHGLIAGATGTGKTVTLQVLA
                     ERLSGAGVPVFLADAKGDLSGLGVPGTTSEKFDARLARLGITDWAPDAASVVFWDVFG
                     KSGHPVRATVSDMGPLLLARLLNLNDTQAGVLQVVFRYADDEGLLLLDLKDLRAMVAH
                     VADNASALKTRYGNVSAASVGAIQRGLLSLDEQGGDSFFGEPMLDIADLMQTDSRGRG
                     VINVLAAEALMNAPRLYSTFLLWLLAELFEQLPEVGDLDKPKLVFFFDEAHLLFTEAP
                     PALLEKIEQVVRLIRSKGVGVYFVTQNPLDIPDRVLGQLGNRVQHALRAFTPRDQKAV
                     RTAAQTLRANPAFDAEAAITELGVGEALVSCLDDKGRPTVVERAFVLPPASRIGPMSA
                     DERQRAIRGSLVYGYYEKTVDRESAFERLAARVTEAAPKPEPQPVVRAPARRTDSLVE
                     SVA"
BASE COUNT          519 a         1061 c         1116 g          553 t
ORIGIN      
        1 cgctcatcgt cggcatcacg cggccttgcg cgcgaggcgc acgccgacga cggcgatgat
       61 catcccggcg acggccggca ggctaagcgt ctcgccgaag atcaggaagg cgatcagcgc
      121 ggtggtcggc ggcgtcagaa agaacaagct ggcgacgtgc acggcgctgc cctggcggat
      181 catcaggttg agcaggctga tggcgccgag cgagagcacg ccgaccagcc aggcgagtgc
      241 gaagacaaac tcgccgttcc agtcgatcgc cagcgattcg aagagcgcga cgccgatgcc
      301 ggtggcgatc gcggtcggca ggaactggat gaccgcgccg gtgcgcaaat cgaagtgggc
      361 gcaaaagcgc ttctggtaga gcgtgccggc ggtgatgccg agcagggcag ccagcgccgg
      421 gagcaacatg ggcagcagtt cggccagcgg tcgatcgagc gcgaggtcgg caccgaccac
      481 cagcgcggtg ccggcgaggc cgagccagag gccgagccac tgccggccgc tgaccgcgtc
      541 gcgcagcagc cagccggcgc ccagcgcggt gaggataggc tgcaggccga ccagcaggct
      601 ggtgacgccg gccggcagtc cgtggtcgat ggcgatgaag acgccgccga ggtaggtggc
      661 gtggacgagt aggccgctga cgccaatgtg cagccactga tgcggatcgc gcggccaggg
      721 cgcgcgggtc gccagcgcca gcatcgtcat cagcgccagc accaagagat aacgtactaa
      781 aaggaaggag agcggttcgg cgtgcggcag gccgtacttg gcgccgatga acccggtgct
      841 ccagagaaag acgaagagca gcggggcgaa gcggaccagc gtggcggtgc taggcggcaa
      901 gatgatcagc cgccggcgag ggacaggcag cggcttgaat cgcggcgcga ccggctggcg
      961 ctcactcgtc gatgtcggcc gtggtgtaga cctcctggac gtcgtcgagg tcgtcgatgg
     1021 cgtcgaggag cttctgcatc ttttcagcgt cttcgccggt caacggggtt tcgttgaggg
     1081 gcttcatcgt cacctcggcg aactccgcgg tgaagcctgc ggcctcgacg gcctccttga
     1141 cgctggcgaa ttcgccggtc ggcggggtca gcacctcgat cgatccgtcc tcgttgatcg
     1201 cgacatcctc ggcgccggcc tcgatggcgg catccatcag tttcgcttcg tcggtgccgg
     1261 gcgcgaagat cagctggccg cagtgcttga actggaagga caccgaaccc tcggtaccca
     1321 tgttgccgcc gtgcttggcg aaggcgtggc ggacttcggc gacggtgcgc gtcttgttgt
     1381 cggtcagaca gtcgaccatc accgccgcac cgccgatgcc gtagccctcg tagcggattt
     1441 cttcgtagct gacgccctca agctggccgg tgccacgctt gatcgcgttc tcgatgttgt
     1501 ccttgggcat cgattcgccc ttggccttgt cgatcgccag ccgcagccgc ggattcatgt
     1561 tggggtcgcc gccgcccatc ttggcggcaa ccgtgatttc cttgatcagc ttggtgaaga
     1621 ccttgccgcg cttggcatcc tgacgcccct tgcggtgctg gatgttggcc catttgctgt
     1681 gtcccgccac gttctgcctc ttctgttctg ctgcgtctgc tggcttggcg cctgcttcgt
     1741 cgccccggtc cggtgtgcga aataatgccc gcggtcggag aaacgggcgc gctaaaccca
     1801 tgaaatcgcg cgaattggtt gataatcagg gggcgaatct tagcatgcgc cccctcgatc
     1861 aagccgccgg aatcagccac ggagccgcca tgtccgaccc gattgtcata gcccgtcagg
     1921 gcgatcgcga gattgccctg ctgccccagt tcgccaaccg tcacgggctg atcgccggtg
     1981 ccaccggtac cggcaagacc gtcacgctgc aggtgctcgc cgaacggcta tcgggcgccg
     2041 gcgtgccggt cttcctcgcc gacgccaagg gggacctgtc gggcctgggt gtgccgggga
     2101 cgacatcgga gaagttcgac gcccggctgg cgcgcctcgg catcaccgac tgggcgcccg
     2161 atgcagcgtc ggtggtgttc tgggatgtct tcggcaagag cgggcatccg gtccgtgcga
     2221 cggtatcgga catggggccg ctactgctcg cgcggctgct caacctcaac gacacgcagg
     2281 ctggcgtgct ccaggtggtt tttcgctacg ccgacgacga aggcctgctg ctgctcgatc
     2341 ttaaggacct gcgggcgatg gtcgcccacg tcgccgacaa cgcctccgcg ctgaagacgc
     2401 gctacggcaa tgtctcggcc gcatcggtcg gcgccattca gcgtggcctg ctgtcgctcg
     2461 acgagcaggg cggcgatagc ttcttcggcg agccaatgct cgacatcgcc gacctgatgc
     2521 agaccgacag ccgcgggcgt ggtgtcatca acgtgctcgc tgccgaggcg ctgatgaacg
     2581 cgccgcggct ctattcgacc ttcctgctct ggctgctcgc cgagctgttt gagcagttgc
     2641 ccgaggtcgg cgatctcgac aagcccaagc tggtgttctt cttcgacgag gcgcacctgc
     2701 tgtttacgga agcgccgccg gcgctgctcg agaagatcga gcaggtcgtg cgcctgatcc
     2761 gctcgaaggg cgtcggcgtc tatttcgtga cgcagaatcc gctcgacatc cccgaccgcg
     2821 tgctcggcca gctcggcaac cgcgtccagc atgcgctgcg agccttcacc ccgcgcgacc
     2881 agaaggccgt gcgcacggcg gcgcagactt tgcgcgccaa tccggctttc gatgccgaag
     2941 cggcaattac cgaactcggc gtcggcgagg cgctggtttc ctgtctcgac gacaagggac
     3001 gcccgactgt cgtcgagcgc gccttcgtgc tgccgccagc ctcgcgcatc gggccgatga
     3061 gcgccgacga gcggcagcgg gccattcgtg gctcgctggt ctacggctac tacgaaaaga
     3121 ccgtcgatcg tgagtcggcc ttcgagcgcc tggcggcccg cgttaccgag gccgcgccga
     3181 aacccgagcc acagccggtc gtacgggcgc cggcccggcg caccgactcg ctggtcgagt
     3241 cggtcgcca
//
LOCUS       DATDAA010000466         4214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_244081,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000466 DATDAA010000000
VERSION     DATDAA010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244081"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..936
                     /gene="era"
                     /locus_tag="VIS73_14225"
     CDS             28..936
                     /gene="era"
                     /locus_tag="VIS73_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY5764362.1"
                     /translation="MSKAELPEEDSRKSGTIAIVGRPNVGKSTLLNALIGEKLSIISR
                     KPQTTRHRISGVLTDADSQFVFVDTPGFQTRHKSALNRAMNRGVTDALADVDVIVLVI
                     EAGRFVSEDQAVERLLPAGKPVVLVINKTDRLSDKARVMNFIGEMAKVRDWAAIIPTS
                     AKHQRQVDAVLGELRALLPQTGLLFPEDEFTDRSERFIAAEYLREKAFRLLGDELPYS
                     LAVDIEKFEVEGNLRRIFAAIIVERDSHKAMVIGDKGETIKRMASEARGDMERLFGGP
                     VYLEVWVKVKSGWADDERMLRSLGYQ"
     gene            955..1665
                     /gene="recO"
                     /locus_tag="VIS73_14230"
     CDS             955..1665
                     /gene="recO"
                     /locus_tag="VIS73_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY5764363.1"
                     /translation="MGKKRVDDEPAFVLHSYPFRETSLIVEAWSRNHGRVALLARGAR
                     RPRSAIRGLLLAFQPLELSWAGQGEVMTLMKAEWVGGQPMLGGAALFCGYYLNELVLK
                     LLPREDAHERLYDRYAETLVRLTNGVEEASLRSFETDLLRELGYGLSLARDTEGRPID
                     PDSQYRYAIERGAERVEASAGSDLISGKTLVDLAAGAYGDPKTLAQSKRLMRSLINHY
                     LAGRELASREIYRELQDL"
     gene            1662..2414
                     /locus_tag="VIS73_14235"
     CDS             1662..2414
                     /locus_tag="VIS73_14235"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY5764364.1"
                     /translation="MIELGVNIDHVATLRQARKTYEPDPLWAAVEAHLGGADGITVHL
                     REDRRHINDDDVRRLRDLTQVKLNLEMAATEEMVGIACRLKPEMAMLVPEGRHEVTTE
                     GGLDIVAQESRLRDAVARLADAGIVTSVFIDAEIAQVDAAARIGARVCEIHTGPYAHA
                     FHHEGRDVESAAVQAELAKIRAAGEAILGHGMRFNAGHALNYFNVQSVAAMPGVRELH
                     IGHAIVSRAVFTGLRAAVAEMKRLMREAAHSP"
     gene            2411..2806
                     /gene="acpS"
                     /locus_tag="VIS73_14240"
     CDS             2411..2806
                     /gene="acpS"
                     /locus_tag="VIS73_14240"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY5764365.1"
                     /translation="MIFGVGTDLVAIARLTALHQRHGARAARRILAASEYAELTQRLA
                     TGFDDAGRFLAKRFAAKEAFAKAMGTGVRAPILLASIAVEHDSLGKPSLAFSGDAEVL
                     MRERRLRTHLSLSDEADFVVAFVIVEQTT"
     gene            2803..3831
                     /gene="nagZ"
                     /locus_tag="VIS73_14245"
     CDS             2803..3831
                     /gene="nagZ"
                     /locus_tag="VIS73_14245"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY5764366.1"
                     /translation="MNRLGPLMIDLAGLELDGGERERIRHPLVGGVILFARNYADPAQ
                     LRELTRQIRETRPVLIAADHEGGRVQRFREGFTRLPPMGELGRAYGDDPQQACDAATR
                     LGYVAAVELRAAGIDLSFAPVLDLDYGVSTVIGDRAFHSTAAGVTALAGAYVAGLHLG
                     GLGACGKHFPGHGYIAADSHDEIPVDTRSFAALEADLQPFLQVPLDAVMPAHVIFPEV
                     DDQPAGFSRRWIELLKNRYNFRGLLFSDDLSMAAAGVAGEAPARVEAAWQAGCDMLLL
                     CNSPEQVDRVLDGWQPTPRGFPVHSVDAIRALPMSAAAQACCRSGDYYRQAVNLAGEL
                     CARSESRA"
     gene            complement(3882..>4214)
                     /locus_tag="VIS73_14250"
     CDS             complement(3882..>4214)
                     /locus_tag="VIS73_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY5764367.1"
                     /translation="ADPMYVFMDADFNQFEVESENMVDALKYLEDGLACEVVFYNGKA
                     ISVELPNSVVREVVYTEPAVKGDTSGKVMKPARLATGHELPVPAFVEIGDKIEIDTRT
                     DEYKNRVK"
BASE COUNT          732 a         1319 c         1392 g          771 t
ORIGIN      
        1 tggcgctgca ccaactggaa gacgagcatg agcaaggctg agttgccgga agaggattcc
       61 cgaaagtcgg gcacgatcgc aatcgtcggg cgtccaaacg ttggcaagtc gacgctgctc
      121 aatgcgctga tcggcgagaa gctcagcatc atctcgcgca agccgcagac gactcggcac
      181 cggatctccg gtgtgctgac tgacgccgat tcccagttcg tctttgtgga cacgcccggg
      241 ttccagacgc gccacaagag cgcgcttaat cgggcgatga atcgtggcgt gaccgacgcg
      301 cttgccgacg tcgacgtcat cgtgctggtc atcgaggccg gccgcttcgt cagcgaggat
      361 caggccgtcg agcgcctgct gcctgcgggc aaaccggtgg tgctggtcat caacaagact
      421 gaccggctga gcgacaaggc gcgggtcatg aactttatcg gcgaaatggc gaaagtgcgc
      481 gactgggcgg cgatcatccc gactagcgcc aagcaccagc ggcaggtcga tgccgtgctc
      541 ggcgaattgc gcgcgctgct gccgcagacc ggcctgctgt tccccgaaga cgagttcacc
      601 gatcgcagcg agcgcttcat tgccgccgaa tacctgcgcg agaaggcgtt ccgcttgctc
      661 ggcgacgaat tgccctacag cctcgccgtc gacatcgaga agttcgaggt cgaaggaaac
      721 cttcggcgga ttttcgccgc gatcattgtc gagcgcgata gccacaaggc gatggtcatc
      781 ggcgacaagg gcgagacgat caagcgaatg gccagtgagg cgcgcggcga catggagcgc
      841 ctgttcggcg ggccggtcta tcttgaagtc tgggttaagg tgaagtcggg atgggcagac
      901 gacgagcgga tgctgcgctc acttggctat cagtgagctg acgcgacggc accgatggga
      961 aagaagcgcg tcgatgacga accggccttc gttctccatt cctatccgtt tcgcgagacc
     1021 agcctgatcg tcgaggcgtg gtcgcgcaat cacgggcggg ttgcgctgct ggcgcgcggc
     1081 gcgcggcggc cacggtcggc catccgcggt ttgctgctgg cttttcagcc actcgagctg
     1141 tcttgggccg ggcaggggga ggtcatgacc ctgatgaagg ccgagtgggt cggcggccag
     1201 cccatgctcg gcggtgccgc actgttctgt ggctactacc tcaacgaact ggtgctgaag
     1261 ctcctgccgc gtgaggatgc gcatgaacgg ctctacgatc gctatgcgga aacgctggtc
     1321 aggctgacta acggcgtcga agaggcgagc ctgcgcagct tcgagaccga tctgctgcgt
     1381 gaactcgggt atggattgag tctggcgcgt gatacggaag gacggcctat cgatcccgat
     1441 tcgcagtatc gctacgcgat cgagcgaggc gccgagcgtg tcgaggcgag tgccggcagc
     1501 gatctgatca gcggcaagac cctcgtcgat ctggccgccg gcgcgtatgg cgatccgaag
     1561 acactggcgc aaagcaagcg cctgatgcga tccttgatca atcattattt ggccggccgc
     1621 gaactggcga gccgggaaat ctaccgggag ctgcaggacc tatgatcgaa ctcggcgtca
     1681 acatcgacca tgtcgccacg ctgcgtcagg cgcgcaagac ctacgaaccc gatccactct
     1741 gggcggccgt cgaggcccac ctgggtggcg ccgacgggat taccgttcac ctgcgcgagg
     1801 atcgtcgcca catcaacgac gacgatgtgc gccgcctgcg cgacctgacg caggtcaagc
     1861 tcaatctgga gatggcggcg accgaggaaa tggttggcat cgcctgtcgg ctcaagccgg
     1921 aaatggctat gctggttccc gaggggcgac atgaagtgac caccgagggt gggctggata
     1981 tcgtcgccca ggagtcgcgg ttgcgcgatg ccgtggcccg gcttgccgac gccggcatcg
     2041 tcaccagcgt cttcatcgac gccgaaatcg cgcaggtcga tgccgccgcg cgaatcggtg
     2101 cccgcgtctg cgagatccat accggaccct acgcccacgc ctttcaccat gaggggcgcg
     2161 acgttgaaag cgcggcagtg caggccgagc tggcgaagat tcgcgcggca ggcgaagcca
     2221 tcctcggcca cggcatgcgt ttcaacgccg gccacgcgct caactacttc aacgttcagt
     2281 cggtcgccgc gatgccgggc gttcgcgaac tgcacatcgg ccacgcgatt gtcagccgcg
     2341 ccgtatttac cggcctgcgc gcggccgtcg ccgagatgaa gcgcctgatg cgggaagccg
     2401 cgcattcgcc atgatcttcg gcgtcggcac cgatctcgtt gccattgcgc gcctgaccgc
     2461 gttgcaccag cggcacggtg ctcgcgcggc gcgccgcatt ctcgcggcga gcgagtacgc
     2521 tgagctgacg cagcgactgg caaccggttt cgatgatgcc ggccgctttc tcgccaagcg
     2581 ctttgccgcc aaggaggcct ttgccaaggc aatgggcacc ggcgtgaggg cgcctatcct
     2641 actggcctcg atagctgtcg agcatgattc gctcggaaag ccgtcgttgg ctttttccgg
     2701 cgatgccgaa gtgctgatgc gcgaacgacg tttgcgcacc catctttccc tgagcgacga
     2761 ggcggacttc gtcgtcgcct ttgtcattgt ggagcagacg acatgaaccg actcggaccc
     2821 ctgatgatcg atcttgccgg gcttgagctc gacggcggcg agcgtgaacg gattcgtcac
     2881 ccgctggtcg gcggcgtgat cctgttcgcc agaaactacg ccgatccggc gcagttgcgc
     2941 gagctgacca gacagatccg cgagacgcgg ccggtactga ttgccgccga ccacgaaggc
     3001 ggacgtgtac agcgttttcg tgagggtttc acacgcctgc caccgatggg cgaactcggc
     3061 cgcgcctacg gcgatgatcc gcagcaggcc tgtgatgcgg cgacgcgtct gggttacgtc
     3121 gcggccgtcg aactgcgggc ggcgggaatc gatctctcgt tcgcgccggt gctcgacctc
     3181 gattacggcg tcagcaccgt gatcggcgac cgcgctttcc actcgactgc ggccggggtc
     3241 acagcgctgg ccggcgccta cgttgccggc ctgcatctgg gcggcctcgg cgcgtgcgga
     3301 aagcacttcc ccggtcacgg ctacattgcc gccgactcgc atgatgagat tcccgtcgac
     3361 acgcgctcgt tcgccgcgct ggaggccgat ctccagccct tcctgcaggt gccactcgac
     3421 gcggtgatgc ccgcgcacgt gatcttcccc gaggtcgatg accagcccgc cggattttcg
     3481 cggcgctgga tcgaactgct gaagaatcgc tacaacttcc gtgggctgtt gttcagtgat
     3541 gacctgtcga tggctgccgc cggcgttgcc ggtgaggcgc cggcgcgcgt cgaggccgcc
     3601 tggcaggcag gctgcgatat gctcttgcta tgcaattcgc cggagcaggt cgatcgggtg
     3661 ctcgacggct ggcagccgac gccgcggggc tttcccgtgc acagtgtcga cgcgatcagg
     3721 gcgctgccga tgagtgctgc cgcccaggcg tgttgccgct cgggcgacta ttaccgccag
     3781 gccgtgaacc tggccggcga gttgtgcgcg cgcagcgaat cgcgcgcctg aaaccaagat
     3841 ggcggccgta gccgccatcc tgagagccca gctgccgcgt tctacttgac gcggttcttg
     3901 tattcgtcgg tacgcgtgtc gatttcgatc ttgtcgccga tttcgacgaa ggccggaacc
     3961 ggcagttcgt ggccagtcgc cagtcgggcc ggtttcatga ccttgccaga ggtatcaccc
     4021 ttgaccgccg gttcagtgta gacgacttcg cggaccaccg agttcggaag ttcaaccgaa
     4081 atcgccttgc cgttgtaaaa caccacctcg caggctagcc cgtcttcgag gtacttgagc
     4141 gcgtcaacca tgttctcgga ctcaacctcg aactggttga agtcggcatc catgaagacg
     4201 tacatcgggt cggc
//
LOCUS       DATDAA010000467         8501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_68364,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000467 DATDAA010000000
VERSION     DATDAA010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68364"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VIS73_14255"
     CDS             <1..513
                     /locus_tag="VIS73_14255"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEY5764368.1"
                     /translation="VGDCTHDTVLKAARIDTARALLVCLTRDDSTTLTVLSARHLNPD
                     LRVLATVREEENITSVERAGAQVVIAQQRIGGFLLAAGVESEVVVPALVELGSARGRM
                     HVEEAEPNADEMGKPPSEVSRGLVLGIWRDKRFVDFDEARELKLRTGDKLVLIVTNRT
                     NPPGGNGTAD"
     gene            614..2077
                     /locus_tag="VIS73_14260"
     CDS             614..2077
                     /locus_tag="VIS73_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5764369.1"
                     /translation="MRFIDLGLHPDILRAIVDQGYTTPTPIQAQAIPIVAAGRDLMGG
                     AQTGTGKTAAFTLPLLHRLSGYATSNPSPARHPVRALILAPTRELAVQVHDNLRDYGK
                     YLPFRTLCAYGGVDIRPQIEEIRKGVEFLVATPGRLLDLVEQKLLHFNSVEALVLDEA
                     DRMLDMGFIPDVQRILNLLPKNRQSLLFSATFSEEIKKLAATMLRDPVTIQVAARNTV
                     SDTITHRVYQVAENDKRSLLIHLLAGDEIPQSLVFVRTKQGCGRLARELVRAGVSADA
                     IHGDKSQIERMKALDAFKQGSVRVLVGTDVAARGLDIDELPYVINYELPHVPEDYVHR
                     IGRTGRAGKLGNAISLVCDHEMGYLADIEKLIKRPIERMALPGFESDGVASGSSRAAG
                     SGRGGRDRHEGGKRPEGGSRGRHGEAKYETPRPRREHAPRPPERKSTVAADGFDYSKP
                     YEPSPAAAPLDDKPHPKPDPHKPKRPVAAILGGFGKR"
     gene            complement(2097..2858)
                     /locus_tag="VIS73_14265"
     CDS             complement(2097..2858)
                     /locus_tag="VIS73_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY5764370.1"
                     /translation="MRLAPVTIDACAVQHIGDRREQQDRLGLFQHPKLKKIALAVVAD
                     GMGGHAGGALAAEQVVLTAKNSLEQYSPREDEPQQMLKSCLEEGHFLIRSHRFVNEQD
                     PHSTGVMLLIEPDRLSWAHCGDSRLYRFRDKRCISHTRDHSFVEELVRRGKATAEEAA
                     EHPNRNLLVTSLGGNEAPQIAVDQVTDLEAGDAFLLCSDGLWGYFKPDELARTVCSMR
                     SREAAAQLMEVARLRGKGAGDNISLIVLRFLASPA"
     gene            complement(2858..3157)
                     /locus_tag="VIS73_14270"
     CDS             complement(2858..3157)
                     /locus_tag="VIS73_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5764371.1"
                     /translation="MSTGVGGGGGQGGERRQNPALRETFDAAYPLIEPFLDPDAGWGG
                     HSLEHLAFRVLRENFPALSVSEVHVLVVAAHRVFIDRHPERSSHLKRPHELQRPR"
     gene            complement(3209..4435)
                     /gene="glcF"
                     /locus_tag="VIS73_14275"
     CDS             complement(3209..4435)
                     /gene="glcF"
                     /locus_tag="VIS73_14275"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEY5764372.1"
                     /translation="METKLAEFVRDTPAGREAEKILRACVHCGFCTATCPTYQITGDE
                     LDGPRGRIYLIKGFLEGKAVTETTRRHLDRCLSCRSCETTCPSGVTYHRLLDIGRDLM
                     NEHAPRPLPGRMLTAAVRESVRNRFVFGTLLALGKIARTLLPVPFAGKIPDQYPVAPW
                     PKSRHPRRYLTLRGCVQETLAPSINLAAAKVLDRAGVALMMAPKDGCCGALRLHGDDR
                     RGGLNDLRALIDRWWPFVAEGQVEGIAISASGCGAHLREAGQLLADDPDYAEKAATIA
                     GQVRDLSEVIADHVELLRERLSPPAEPTPIAFHSPCTLQHGLGVRGVAESLLVAAGYR
                     LVPVEESHLCCGSAGIYALLHPLMSGRLRERKLEALCVSKPRFIATANIGCLTQLSGG
                     EVPVRHWIELLADRLP"
     gene            complement(4435..5505)
                     /gene="glcE"
                     /locus_tag="VIS73_14280"
     CDS             complement(4435..5505)
                     /gene="glcE"
                     /locus_tag="VIS73_14280"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEY5764373.1"
                     /translation="MSEADLRHWQSLVRAAARANRKLRVRGFGSKDFYGGELEGEVFD
                     TREHAGVVYYEPTELVVTARCGTPLAELEATLAGQGQWLPCEPPHFGGASVGGMVAAG
                     IAGPARMAAGGIRDYVLGVRVIDGNGELLRFGGEVMKNVAGYDVSRLYAGSLGTLGVI
                     VDVSLKVLPRPVATETRVLELDREAALATMNQFAGQAVPLTASAWYRGQLFLRFAGSP
                     AAVGKLAKDVGGEVLADAAQFWESLREQTHEFFAGAVAGNESLYRLALPVRAASSVPA
                     GDCLIEWGGAQRWCYGDGERLRETAEQSGGVAVRFRTAGDASRADSMPARHPNIQMIE
                     RRLRKAFDPSGVFSHGRLGVEH"
     gene            complement(5502..6962)
                     /locus_tag="VIS73_14285"
     CDS             complement(5502..6962)
                     /locus_tag="VIS73_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5764374.1"
                     /translation="MSAANEAGELPRGFADALRAILPDSAIRLGVEACRPYECDGLTA
                     YRQVPPVVVLPTTESQVRSVLRLCEHHDVPLVVRGAGTGLSGGALPHATGVLMSLARF
                     MKVISIDPEARTAVVQPGVRNVTVSEAAAPYGLYYAPDPSSQIACTIGGNVAENSGGV
                     HCLKYGLTVHNVLRVRALTIDGEVLEIGGLAPDVPGLDLLSLTIGSEGMLAAVTEITV
                     KLTPKPPHAQVLLASFDDVGQAADAVAAIIAAGVIPAGLEMMDRPAIAAVEPFAKAGY
                     DLSAAAILLCEADGTAEEVADEIARVSAVLEKAGARNLRVSKDEAERLLFWAGRKAAF
                     PAVGRITPDYLCMDGTIPRRRLAEMLTAIQGMEKKYDLRCANVFHAGDGNLHPLIMFD
                     ANDPDSMRRAEAFGGEILELSVALGGTITGEHGVGIEKLKQMCVQFSPEELAAFERVK
                     RAFDPPGLLNPGKVVPTPARCREYRLTGSSREEPAA"
     gene            complement(6959..8098)
                     /locus_tag="VIS73_14290"
     CDS             complement(6959..8098)
                     /locus_tag="VIS73_14290"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5764375.1"
                     /translation="MAKDLGLTMTGLGRRLATAGLALAGTLLLGACGEPEPLRIGFLG
                     GVSGRVADLGIGGRNGAQMAVEAVNRAGGIDGRQVIMVVKDDAQDPEKAARGMREFGQ
                     TGIRLVIGPMTSAMAVAALPVAAEFGILLVSPTVTTDELAGKEDNFVRVLSPASDYAG
                     KSAEYQFKQNGLRRIVVIHDAANAAYVGSWLSAFRTRFTALGGTLLAEVPFTSGQQLG
                     LRELVAKSVRGEADGVLILANSVDAALIAQYARSERPGFPIAMSEWPATERFIELAGA
                     AAEGVTAAQFFDRNSKSPAYEAFRSDFRKRFGVEPGFAGVTGYDATMLALRGLAKSVA
                     TPGPHVVGIERFDGLQGPIKIDRFGDAKRKTFITRVEKGQFMVVE"
     gene            complement(8077..>8501)
                     /locus_tag="VIS73_14295"
     CDS             complement(8077..>8501)
                     /locus_tag="VIS73_14295"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY5764376.1"
                     /translation="QRDCYVDNLRLAAKACAAAGITLLIEPINSRVDMPGFWLDTPDK
                     AIDCLNLTGAENLALQFDVYHAQVMSGDAVAALRRCRTLIGHVQIADCPGRNEPGSGA
                     IDWAGVRAALADYDGWIGCEYRPLADTQAGLEWRRTWG"
BASE COUNT         1490 a         2989 c         2695 g         1327 t
ORIGIN      
        1 gtcggcgact gcacgcacga cacggtgctt aaggcggcgc ggatcgatac cgcccgcgca
       61 ctattggtct gcctgacgcg cgacgactcg acgacgctga cggtgctctc ggcgcgccac
      121 ctcaaccccg acttgcgcgt cctcgccacc gtgcgcgagg aggagaacat cacctcggtc
      181 gagcgtgccg gcgcccaggt cgtcatcgcc cagcaacgga tcggcggctt cctgctcgcc
      241 gccggcgttg aaagcgaagt ggtcgtccct gcattggtcg aactcgggtc ggcacgcggt
      301 cgcatgcatg tcgaagaagc cgaaccgaac gccgatgaaa tgggcaaacc gccgtcggaa
      361 gtaagccgtg ggctggtgct tggcatatgg cgcgacaagc gtttcgtcga tttcgacgag
      421 gcgcgcgagc tgaaactacg caccggcgac aagctggtac tgatcgtcac caaccggacc
      481 aatccgccgg gcggcaacgg caccgccgac tgagcagcct aggcggctcc tgccggccgc
      541 cggccggtat aatgctccgc ttatgcaaaa tacccccgac actgcggtta ccgccgacca
      601 ggccgtgccg ccaatccgct tcatcgacct cggcctccac ccggacatcc tccgcgccat
      661 cgtcgatcag ggctacacga cgccgacacc gatccaggca caggcgattc cgatcgtcgc
      721 cgccggccgc gacctgatgg gtggcgcgca gaccggcacc ggcaagaccg ccgctttcac
      781 gctgccgctc ttgcaccgct tgtcgggcta cgccacttcc aatccgtcgc cggcgcgtca
      841 cccggtgcgc gcgctgatcc tcgccccgac ccgcgaactg gcggtgcagg tccacgacaa
      901 cctgcgtgat tacggcaagt acctgccctt ccgcacgctc tgcgcctacg gcggcgtcga
      961 tatccggccg cagatcgagg agatccgcaa gggcgtcgag ttcctcgtcg cgacgccagg
     1021 gcggctgctc gatctggtcg agcagaagct gctgcacttc aactcggtcg aggcgctggt
     1081 cctcgacgaa gcagaccgca tgctcgacat gggcttcatc cccgatgtcc agcgcattct
     1141 caacctgctg ccgaagaacc ggcagagcct gctcttctcg gcaaccttct ccgaagagat
     1201 caagaagctg gcggcaacga tgctgcgcga tccggtgacg atccaggtcg cggcgcgcaa
     1261 cacggtctcc gacaccatta cccaccgcgt ctatcaggtc gccgagaacg acaagcgctc
     1321 gctcttgatc catctgctgg ccggcgacga gatcccgcaa agcctcgtgt tcgtgcggac
     1381 caagcagggc tgcgggcgct tggctcgcga actggttcgc gccggcgtct cggccgacgc
     1441 catccacggc gataagtcgc agattgagcg catgaaggca ctcgacgcct tcaagcaggg
     1501 cagcgtgcgc gtcctcgtcg gcaccgacgt cgcggcgcgc ggcctcgaca tcgacgagtt
     1561 gccctacgtg atcaattacg aactgccgca cgtgcccgaa gactacgtgc accgtatcgg
     1621 ccgcaccggc cgcgccggca agcttggcaa tgccatctct ctggtctgcg atcacgagat
     1681 gggctacctc gccgatatcg agaagctgat caagcggccg atcgagcgca tggcactgcc
     1741 cggtttcgag tccgatggcg tcgcctcggg cagttcgcgc gcggccggca gcggtcgcgg
     1801 cgggcgtgat cgacacgaag gcggcaagcg gccggaaggc ggttcgcgcg gacgtcacgg
     1861 cgaagcgaaa tacgagacgc cacgtccgcg tcgcgaacac gcaccgcgcc cgcccgaacg
     1921 gaagtcgacg gtcgccgccg acggtttcga ctacagcaaa ccctacgagc cctcgccggc
     1981 cgccgcaccg ctcgatgaca agccgcaccc gaaacccgat ccgcacaagc ccaagcggcc
     2041 ggtcgcggcg atcctcggcg gcttcggcaa gcgctgaggc agaccccgcg ccggcgctag
     2101 gccggcgacg ccagaaagcg caggacgatc agcgagatgt tgtcgcctgc gcccttgccg
     2161 cgcagacgtg caacctccat cagctgcgcc gccgcttccc gtgaccgcat cgagcacacg
     2221 gtccgcgcta gctcgtcggg cttgaagtaa ccccagagcc cgtcggagca gagcaggaag
     2281 gcgtcgccag cctcgaggtc ggtcacctga tcgaccgcga tctgcggcgc ttcgttgccg
     2341 cccagcgaag tgacgagcag gttgcggttg ggatgctcgg cggcctcttc ggcggtggcc
     2401 ttgccgcgcc gcacgagttc ctcgacaaag gaatgatcgc gcgtatgcga gatgcagcgt
     2461 ttgtcgcgaa agcggtacag gcgcgaatcg ccgcaatgcg cccacgacag gcggtcgggt
     2521 tcgatcaaca gcatgacacc ggtgctgtgc ggatcctgct cgttgacgaa gcggtggctt
     2581 cggatcagga aatgcccctc ctcaagacaa gacttgagca tctgctgcgg ctcgtcctcg
     2641 cgcgggctgt attgctcgag gctgtttttc gcggtcaaca ccacctgttc ggcggccagc
     2701 gcccccccgg cgtgtccgcc catgccgtcg gcgacgacgg cgagggcgat cttcttcagc
     2761 tttggatgct ggaacaggcc gaggcgatcc tgctgttcgc gccgatcgcc gatatgctgg
     2821 accgcgcagg cgtcgatggt aaccggagcc aggcgcatca acgcggtcgc tgcaactcgt
     2881 gcggccgttt caggtgcgaa ctgcgctcgg gatggcggtc gatgaagacc cggtgcgcgg
     2941 cgacgacgag cacgtgcacc tcagaaacgc tcaatgccgg aaaattctcg cgcagcacgc
     3001 ggaaagccag atgctcgagc gagtggccac cccaaccggc atcagggtcg agaaagggct
     3061 cgatcagcgg gtaggctgca tcgaaggtct cacgcagcgc cggattctgt cgccgctcgc
     3121 caccctggcc gccaccgcca ccaacacctg tactcatcgc ctgcctccgc tgcccatcat
     3181 ccgtgtcgtc gcagtctagc ccagcgtttc agggcaaacg atctgccagc agctcaatcc
     3241 agtgccgtac cggcacctcg ccgccggaca gctgcgtgag gcagccgatg tttgccgtgg
     3301 ctatgaaacg cggcttgctg acgcacagcg cttcgagctt gcgctcgcgc aggcgaccgg
     3361 acatcagcgg atgcagcaac gcgtagatgc cggccgatcc gcagcacagg tgcgactcct
     3421 cgaccggcac cagccggtag ccggcggcga ccagcagcga ctcggcgacg ccacgaacgc
     3481 ccagcccatg ctgcaaggtg cacgggctgt gaaaggcaat cggcgtcggc tcggccggcg
     3541 gactgagtct ttcgcgcaag agctcgacat gatcggcgat cacttcggag agatcgcgca
     3601 cctggccggc aatggtcgcc gccttttccg cgtagtcggg atcgtcggcg agcagctggc
     3661 ccgcctcgcg caggtgtgcg ccgcaacccg aggccgaaat cgcaatgcct tcgacctgac
     3721 cctcggcaac aaaaggccac cagcgatcga tcagtgcgcg cagatcgttc agcccaccgc
     3781 gccgatcgtc gccgtgcagg cgcagcgcac cgcagcagcc gtcctttggc gccatcataa
     3841 gcgccacccc ggcgcgatca aggaccttgg ccgccgccag attgatcgat ggcgccagcg
     3901 tttcctgaac gcagccacgc agcgtcaggt agcgccgcgg gtgacgtgat ttcggccagg
     3961 gggcgaccgg atactggtca ggaatcttgc cggcaaacgg taccggcagc agcgtcctgg
     4021 cgatcttacc gagcgccagc agcgtgccga agacgaagcg gttgcggacc gactcgcgga
     4081 ccgccgcggt gagcatgcgg cccggcagcg ggcgtggcgc atgttcgttc atcaggtcac
     4141 ggccgatgtc gagcaaccgg tgataggtaa ccccggaggg gcaggttgtt tcacaggagc
     4201 ggcatgacag gcagcgatcg aggtggcgcc gcgtcgtctc cgtgaccgct ttcccctcaa
     4261 ggaatccctt gatcagatag atccggccgc gcggcccgtc gagctcgtcg ccggtgatct
     4321 ggtaggtcgg gcaggtcgcc gtgcagaagc cgcaatgcac acaggcgcgc aggatctttt
     4381 cggcctcacg gcccgccggc gtgtcgcgaa cgaattcggc gagcttggtt tccatcagtg
     4441 ctcgaccccc agccggccgt ggctgaagac acccgaggga tcgaatgcct tgcgcagtcg
     4501 gcgctcgatc atctgaatgt tcggatgccg tgccggcatg ctgtcggcgc gcgatgcgtc
     4561 gcccgccgtc cgaaatcgaa cggcgacccc acccgactgc tcggcggtct ctcgcagacg
     4621 ctcgccatcg ccatagcacc agcgctgtgc gccgccccat tcgatcaggc aatcgccggc
     4681 cggcaccgaa gatgccgccc tcaccggcag cgccagccga tagagcgact cattgcccgc
     4741 caccgcaccg gcaaagaact cgtgcgtctg ttcgcgcagg ctttcccaga actgcgcggc
     4801 gtcggccagc acttcaccgc ccacatcctt cgcaagtttg ccaaccgcgg ccggcgagcc
     4861 ggcgaagcgc agaaacagtt gaccgcggta ccacgcgctg gcggtcagcg gcaccgcctg
     4921 tccggcaaat tggttcatcg tcgccagcgc cgcctcgcgg tcaagctcaa gcacccgcgt
     4981 ctccgtcgcc accggtcgcg gcagcacctt cagcgataca tcgacgatga cgccgagcgt
     5041 gcccaggctc ccggcgtaga gacgcgacac gtcatagcct gcgacgttct tcatcacctc
     5101 gccgccgaag cgcagcagct cgccattgcc gtcgattacg cgaacgccga gtacatagtc
     5161 gcggattccg cctgcggcca tgcgagccgg cccggcaatg ccggcggcga ccatgccacc
     5221 gaccgaggca ccgccgaaat gcggcggttc acagggcagc cactgtccct gtccggccag
     5281 cgtcgcctcg agttcggcaa gcggcgtacc gcaacgcgcg gtgaccacca gttcggtcgg
     5341 ttcgtagtaa acgacgccgg cgtgttcccg ggtgtcgaaa acctcgccct ccagttcgcc
     5401 gccgtaaaag tctttgctgc cgaaaccacg cacgcgaagc ttgcggttgg cgcgcgccgc
     5461 ggcgcggacc agcgattgcc agtgacgcag atcggcttcg ctcatgccgc aggctcctca
     5521 cgcgacgatc cggtcagccg gtactcgcgg caacgcgccg gcgtcggcac gaccttgccc
     5581 ggattgagca ggccaggtgg atcgaaagcg cgcttgacgc gctcgaaggc ggccagctct
     5641 tccggcgaaa actgcacgca catctgcttg agcttctcga tgccgacgcc gtgctcgccg
     5701 gtgatggtgc cgccgagcgc caccgacagc tcgagaatct cgccgccgaa ggcctcggcg
     5761 cgacgcattg aatcagggtc attggcgtca aacatgatca gcgggtgcaa attaccgtcg
     5821 cccgcatgga agacgttggc gcagcgcaag tcgtatttct tttccatacc ctggattgcc
     5881 gtcagcatct cggccagccg ccgacgcgga atcgtgccgt ccatgcacag gtagtccggc
     5941 gtgatgcgtc cgaccgccgg gaacgcagcc ttgcggccag cccagaaaag caaccgctcg
     6001 gcctcgtcct tcgacacgcg caggttgcgc gcgccagcct tttcgagaac cgccgagacc
     6061 cgcgcaattt cgtcggcgac ttcctcggcc gtgccatcgg cctcgcagag caggatcgct
     6121 gcggcgctga gatcgtagcc cgccttcgcg aagggctcga ccgcggcgat cgccggccgg
     6181 tccatcatct cgaggccggc tggaatcacg ccggcggcga tgatcgccgc taccgcgtcg
     6241 gccgcctgcc cgacgtcatc gaaggaggcg aggaggacct gtgcgtgcgg cggcttcggc
     6301 gtcagcttga cggtgatctc ggtaaccgcc gccagcatcc cttccgagcc gatggtcagc
     6361 gacagcaggt ccagacccgg cacatcgggc gccaacccac caatctccaa cacctcgccg
     6421 tcgatggtca gcgcccgcac gcgcaacacg ttgtgcacgg tcagtccgta cttgaggcaa
     6481 tgcacgccgc ccgaattttc ggcgacgttg ccgcctatcg tgcaggcgat ctgcgaactc
     6541 gggtccggcg cgtaatagag accgtaggga gccgccgcct ccgagacggt gacgttgcgc
     6601 acaccgggtt gcaccacggc ggtccgcgct tccgggtcga tcgagatgac cttcatgaag
     6661 cgcgccagcg acatcaacac accggtcgca tgcggcagcg ccccgcccga aaggccggtg
     6721 ccggcaccgc gcacgaccag tggtacgtcg tggtgttcgc acagccgcaa aacgctgcga
     6781 acctgactct cggtggtcgg cagcaccacc accggcggca cctgtcggta cgcggtcagg
     6841 ccatcgcact cgtagggtcg gcaggcctcg acgccgaggc ggatcgccga gtcgggcagg
     6901 atcgcccgca aggcgtcggc gaaaccccgg ggaagctctc cagcctcgtt cgccgcgctc
     6961 attcgaccac catgaactga cctttctcga cccgagtgat gaaggtcttg cgcttcgcgt
     7021 caccaaagcg atcaatcttg atgggtccct gcaatccgtc gaagcgctcg attccgacaa
     7081 catgcgggcc gggcgtagcg accgatttgg cgagaccgcg cagcgccagc atcgtcgcgt
     7141 cgtaaccggt gacgccggca aagcccggct caacgccgaa acgtttgcgg aagtcagaac
     7201 ggaaagcctc ataggcaggc gacttgctgt tgcggtcgaa gaactgtgcg gccgtcacgc
     7261 cttcggcagc ggcgccggcc agctcgatga aacgctcggt ggccggccac tcggacatgg
     7321 cgatcgggaa ccccgggcgc tcgctgcgcg cgtactgcgc gatcagcgcg gcatcgaccg
     7381 agttggcgag gatcagcacg ccgtcggcct cgccacgcac cgacttggcg accagctcac
     7441 gcagaccgag ctgctggccc gaggtgaagg gcacctcggc gagcagcgtg ccaccaagcg
     7501 ccgtgaaccg cgtccgaaac gccgacagcc agctgccgac gtaggccgca ttggccgcgt
     7561 cgtggatcac cacgatgcgg cgcagaccat tctgcttgaa ctggtattcg gcgctcttgc
     7621 cagcgtaatc gctcgccggc gagagcacgc ggacgaagtt gtcttccttg ccggctagtt
     7681 cgtcggtggt caccgtcggg ctgaccagca ggatgccgaa ttcggcggcg accggcagcg
     7741 ccgcgacggc catcgcgctg gtcatcggcc cgatcaccag ccggatgccg gtctgcccga
     7801 actcgcgcat gccgcgggcg gccttttccg ggtcctgcgc gtcgtccttg accaccatga
     7861 tcacctgccg gccatcgatg ccgccggccc gattgacggc ctcgaccgcc atctgcgcgc
     7921 cgttgcgtcc gccaatgccg aggtcggcga cgcggcccga cacgccgccg agaaaaccaa
     7981 tgcgcagggg ctccggctcg ccgcaggcgc ccagcagcag cgtgccggca agcgccagac
     8041 cggccgtcgc caatcgtctt ccgagtcccg tcatcgtcag ccccaggtcc ttcgccattc
     8101 cagtcccgcc tgcgtatcgg cgagcggccg gtattcgcag ccgatccagc catcatagtc
     8161 ggccagtgcc gcgcgaacgc cggcccagtc gatcgcgccg ctgcccggct cgttacgtcc
     8221 cggacaatcg gcgatctgca cgtggccgat cagcgtccgg caccgacgca aggcggcaac
     8281 ggcgtcgccg ctcatcacct gcgcgtggta cacgtcgaac tgtaacgcaa gattttcggc
     8341 gccggtcagg ttcaggcagt caatcgcctt gtccggcgta tcgagccaga agcccggcat
     8401 gtcgacgcgg ctgttgatcg gctcgatcaa gagcgtgatt cccgccgccg cacaggcctt
     8461 cgccgccagc cgcaggttgt cgacgtagca gtcgcgctgc t
//
LOCUS       DATDAA010000468         4941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_34312,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000468 DATDAA010000000
VERSION     DATDAA010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34312"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1599)
                     /locus_tag="VIS73_14300"
     CDS             complement(88..1599)
                     /locus_tag="VIS73_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEY5764377.1"
                     /translation="MSSPSIAFDHSYARELEGCYVACRPVPVPAPALCYFGDELAEEL
                     RLGLAGHDSAELAAMFSGNALPHGARPIAQAYAGHQFGSFSPSLGDGRALLIGEVIDR
                     YGRRRDLALKGSGRTPFSRNGDGKAALGPMLREVLIGEAMHALGIPTTRALAVVATGE
                     AVRRERPLPGAVLTRAAASHLRVGTFEFFAAHGTPEMLRQLADYAIARHDPELLGRED
                     RYLAFLRAVAERQAALLAQWMHVGFIHGVMNTDNMSIAGETIDYGPCAFLEAYDPQTV
                     FSSIDAGGRYAYAAQPQIAQWNLARLAEALLPLIDDDEARALERAGAVVADFPQPYRR
                     HWLAGLRAKLGFFKPTEDGFDGASARDGDDVALGENWLALLHAQRIDYTLAWGRLADA
                     AAGDETPLRALFAGQPGLDRWLERWRARCAADNAAAAQRAQHMRRVNPWLIPRNHRVE
                     EALAAATDDGDLAPFERLLAALRQPFVEDLARSAYAEPAPADFTANYRTFCGT"
     gene            complement(1928..2680)
                     /locus_tag="VIS73_14305"
     CDS             complement(1928..2680)
                     /locus_tag="VIS73_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock and DUF1294 domain-containing
                     protein"
                     /protein_id="HEY5764378.1"
                     /translation="MLTTYPLLVWRASGALLGTRMRHQGRISNWKGEQGFGFIAPHDR
                     SAPVFVHIKDFSDRRRRPLLGDIVTYEVRPDDRGRLQATDVALADHRSAGPGRASARR
                     GVSRVAIAVCVVAAFAAVVGTGLLPYPLAAVYGAASGLALLAYGLDKSAARANRWRIS
                     ERTLHLFGLAGGWPGSLVAQQLFRHKSSKASFQLAFWTTVVVNCGATAWLLSPSGANS
                     LRSLDRAVSRIAAAVTPADENWIRRAAPRNTD"
     gene            2766..>4941
                     /gene="acnA"
                     /locus_tag="VIS73_14310"
     CDS             2766..>4941
                     /gene="acnA"
                     /locus_tag="VIS73_14310"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY5764379.1"
                     /translation="MTTQQAFSARFRKTFGSGAARRTFYSLPALEAAGIGKVSRLPVS
                     IRIVLEALLRHCDGERVSEEQVRALANWKPKAKREAEIPFVVSRVVLQDFTGVPLLAD
                     LAAMRDAAAKFGADPQKIEPRVPVDLVVDHSVTVDHYGSAQALDLNMKLEFARNQERY
                     RFLKWGTQAFETFRVVPPGTGIVHQVNLEYLSPGVHRDGELLYPDTLVGTDSHTTMIN
                     GIGVVGWGVGGIEAEAGMLGQPVYFLTPDVIGVELAGRLREGVTATDLVLTITELLRK
                     TGVVGAFVEFFGEGGATLSVTDRATIANMAPEYGATMGFFPVDEKTAAYLTQTGRSPE
                     SVADFSAYFKAQGLFGMPMPGDIAYTKTLRLDLATVTPSLAGPRRPQDRIEIGAVKSR
                     FAKLHRAPADQGGYGKDKTTLARRYPLADGQTLGDGDVLIAAITSCTNTSNPSVMLAA
                     GLLAKKAAARGLTVNPRIKTSLAPGSRVVSDYLERAGLMQPLQQLGFHLAGYGCTTCI
                     GNAGDLTPAINAAIAEHDLVCAAVLSGNRNFEARIHPNLRANFLASPPLVVAYAIAGS
                     MNVDLMTEPLGTGADGQPVWIGDVWPSGAEIEAALHHATDPAAYRRRYAGKLETHPLW
                     QQIEGEAGATYGWPKSTYIARPPFFDGFAAEPTPPTAISGARALAIFGDSVTTDHISP
                     AGSIKSDSPAGQWLRDHGVQKADFNSFGARRGHHEVMIRGTFG"
BASE COUNT          832 a         1715 c         1654 g          740 t
ORIGIN      
        1 cgggtacggg gagcggattc gggtggggtg aggcggatag gccttacctt gctggccgga
       61 ccacgggcac ctggcgccaa ggccgcgtca ggttccgcag aaagtgcggt aattggccgt
      121 gaagtcagcc ggcgccggtt cggcgtaggc cgaccgcgcc aggtcttcga cgaagggctg
      181 gcgcagcgcc gcaagcaggc gctcaaaggg cgccagatcg ccgtcgtcgg tggccgccgc
      241 caaggcttcc tcgacacggt gattgcgcgg gatcagccag ggattgaccc ggcgcatgtg
      301 ctgggcgcgc tgcgccgccg ctgcattgtc ggcggcgcaa cgcgcgcgcc agcgttcgag
      361 ccaacgatcc agccccggct gtccggcaaa cagcgcgcgc agcggtgtct cgtcgccggc
      421 ggctgcatcg gccagccgcc cccaggcgag cgtgtagtcg atccgctgcg cgtgcaacag
      481 cgcgagccag ttttcgccga gcgcgacatc gtcgccgtcc cgagcgctcg cgccgtcaaa
      541 accgtcttcc gttggcttga agaacccgag cttggcacgc agtccggcga gccagtgccg
      601 gcggtagggc tgcgggaagt ctgctaccac agcgccggcc cgctccagcg cacgggcctc
      661 gtcatcgtcg atgagcggca gcagtgcctc ggccagccgc gccaggttcc actgcgcgat
      721 ctgcggctgc gccgcgtagg cgtagcgccc gccggcgtcg atcgagctga acacggtctg
      781 cggatcgtag gcttcgagga aggcgcaggg gccgtagtcg atcgtttcgc cggcgatgct
      841 catgttgtcg gtgttcatca cgccgtgaat gaagccaacg tgcatccact gcgccagcag
      901 cgccgcctgg cgctcggcca cggcgcgcag gaaggccaag taacggtctt cgcgcccgag
      961 cagctcggga tcgtggcggg cgattgcgta gtcggcgagc tgccgcaaca tctcgggcgt
     1021 accgtgcgcg gcaaagaact cgaaggtacc gacacgcagg tggctggcgg cagcgcgcgt
     1081 gaggacggcg ccgggcagcg gccgctcgcg gcggaccgct tcgccggtgg caaccaccgc
     1141 caaggcgcgc gtggtgggaa tccccagcgc gtgcatcgcc tcgccgatca gcacctcgcg
     1201 cagcatcggc cccagcgccg ccttgccatc gccgttgcgc gagaacgggg tacgcccgga
     1261 gcccttgagc gcgaggtcgc gccggcgtcc gtagcggtcg atcacctcgc cgatgagcag
     1321 tgcgcgaccg tcgcctaggc tgggcgagaa gctcccgaac tggtggccgg cataggcttg
     1381 cgcgatcggc cgcgcgccgt gcggcagcgc attgccggaa aacatcgcgg ccaattcggc
     1441 cgagtcgtgg ccggccaacc ccagccgaag ctcctcggcg agttcgtcac cgaaatagca
     1501 caacgccggc gccggcacgg gtaccggtcg gcaggcgacg tagcaaccct ccagctcgcg
     1561 cgcgtagctg tggtcgaagg caatgctggg cgatgacatg gggcttttcg gtatccggcg
     1621 gtgattgcgg tgcccggaca gtggcaaccg cgctgctgtt cgaccgctgc cggcggtttg
     1681 gttcgcgcgg cgtgaatttg ccgctgacgc gggcctgcca ctactggaaa tgcacagcga
     1741 gaccgctcag caagatggca actacgacca tcgccacgag ctcgaggtca tgcgcctgtc
     1801 ccggcggcgg gaatcgccca ccggtaagca ccctggagag caggcgtccg ggtcagaaga
     1861 agactgcggc caacaagatc ggcaccacga cgatctggac gaaggattcc acgaaagtga
     1921 cgtcctgcta gtcggtgttt cgcggtgccg cgcgccgaat ccagttctcg tcggccggtg
     1981 tgaccgctgc ggcgattcgc gacacggccc ggtcgagcga tcggagggaa ttggcgccgg
     2041 acggtgacag caaccaggcc gtcgccccgc aattgacgac gacggtagtc cagaaggcga
     2101 gctggaacga cgccttgctc gacttgtgac ggaacagctg ctgggccacc agcgaaccgg
     2161 gccaaccacc tgccagcccg aacagatgca aggtcctctc tgagatacgc caccggtttg
     2221 cacgtgccgc cgacttgtcg agaccgtagg cgagaagggc gagcccgctg gcggccccgt
     2281 agaccgcagc cagcggatag ggcaacaggc cggtgccgac cactgccgca aatgcggcca
     2341 ccacgcagac cgcaatggcg actcgagaca cgccgcgccg cgcactggcg cggccgggac
     2401 ccgctgaccg gtgatcagcg agcgcgacgt cggtcgcctg aagccggccc cggtcgtccg
     2461 gtcgaacttc gtaggtcacg atgtccccga ggagcggccg tcgccgtcgg tcgctgaagt
     2521 ccttgatgtg gacgaagacc ggcgcactcc ggtcgtgcgg tgcgatgaat ccgaatccct
     2581 gttcgccctt ccagttcgaa atcctgcctt gatgacgcat tcgtgttccc aggagtgctc
     2641 ccgacgctcg ccaaacgagc aatggatagg tcgtcagcat actggatgcg gtacgcgggg
     2701 cgacactggg aaggaactgc ggctcctcgt ctgcgtctga tcatagccac ccaaaaggat
     2761 ctcccatgac cacgcaacaa gccttcagcg ctcgcttccg caagaccttc ggcagcggcg
     2821 ccgcgcgccg caccttttac agcctgccgg cgctcgaagc cgccggcatc ggcaaggtgt
     2881 cgcggctgcc ggtgtcgatc cgcatcgtcc tcgaagcgct gctgcggcat tgcgacggcg
     2941 agcgcgtcag cgaggaacag gtgcgcgcgc tggcgaactg gaagccgaaa gccaagcgcg
     3001 aggcggagat tcccttcgtt gtctcgcgtg tggttctgca ggacttcacc ggtgtaccgt
     3061 tgctggccga cctggcggcg atgcgcgacg cggcggcgaa gttcggtgcc gatccgcaga
     3121 agatcgagcc gcgggtaccc gtggatctcg tcgtcgacca ttcggtaacg gtcgatcact
     3181 acggcagcgc ccaggcgctc gatctcaaca tgaaactcga gttcgcgcgt aatcaggaac
     3241 gctaccgctt cctcaagtgg ggcacacagg ccttcgagac cttccgcgtc gtgccgcctg
     3301 gcaccggcat cgtccatcag gtcaatctcg aatacctatc gccgggcgtc caccgcgatg
     3361 gcgagctgct ctatcccgac acgctggtcg gcaccgacag ccacacgacg atgatcaacg
     3421 gcatcggcgt cgtcggctgg ggcgtcggcg gcatcgaggc cgaggccggc atgctcggcc
     3481 agccggtgta cttcctgacc cccgacgtga tcggcgtcga actcgccggt cggctgcgcg
     3541 agggcgtcac cgccaccgat ctggtgctga cgatcaccga actgctgcgc aagaccggcg
     3601 tggtcggcgc cttcgtcgaa ttcttcggcg aagggggagc aacgctctcg gtgaccgacc
     3661 gcgcgacgat cgccaacatg gcgcccgagt acggcgcgac gatgggcttc ttcccggtcg
     3721 acgagaagac cgctgcctac ctcacgcaga ccggccgcag ccccgagtcg gtcgccgact
     3781 tctcggccta cttcaaggcg caggggctgt tcggcatgcc gatgcccggc gacatcgcct
     3841 acaccaagac gctgcggctc gacctcgcaa cggttacgcc ttcgctcgcc gggccgcgcc
     3901 ggccgcagga ccgcatcgag atcggtgccg tgaagtcgcg cttcgccaag ctgcaccgcg
     3961 cgccggccga ccaaggcggc tacggcaagg acaagacgac gctcgctcgc cgctacccgc
     4021 tcgccgacgg gcagacgctc ggcgacggcg acgtgctgat cgccgcgatc acctcgtgca
     4081 ccaacacctc gaacccgagc gtgatgcttg ccgccggcct gctggcgaag aaagccgcgg
     4141 cacgcgggct gaccgtgaac ccgcggatca agacctcgct ggcgccgggc tcgcgcgtgg
     4201 tttccgacta tctcgaacgg gctggactaa tgcagccctt gcagcagctc ggctttcacc
     4261 tcgccggcta tggctgcacg acctgcatcg gcaatgctgg cgacctgaca cccgcaatca
     4321 acgcggcgat cgccgagcac gacctcgtgt gcgctgccgt gctctcgggc aaccgcaact
     4381 tcgaggcgcg catccacccc aacctgcgcg ccaacttcct cgccagcccg cccttggtcg
     4441 tcgcctatgc gatcgccggt agcatgaacg tcgacctgat gaccgagcca ctcggcaccg
     4501 gtgccgacgg ccagccggtg tggatcggcg atgtctggcc gagcggcgcc gagatcgagg
     4561 cggcgctgca ccacgccacc gacccggccg cctaccgccg acgctacgcc ggcaagctcg
     4621 aaacgcatcc gctgtggcag cagatcgaag gcgaggcagg cgccacctac ggctggccta
     4681 agtcgaccta catcgcgcgg ccgcccttct tcgacggctt cgccgccgag ccgacgccgc
     4741 cgaccgcgat cagcggcgcc cgggcgctgg cgatcttcgg cgactcggtg accacagacc
     4801 acatctcgcc ggccggttcg atcaagtccg actcaccggc cggccaatgg ctgcgcgacc
     4861 atggtgtaca gaaggccgac ttcaactcct tcggcgcccg gcgcggccac cacgaggtga
     4921 tgatccgcgg caccttcggc a
//
LOCUS       DATDAA010000469         2218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_68646,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000469 DATDAA010000000
VERSION     DATDAA010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68646"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VIS73_14315"
     CDS             complement(<1..267)
                     /locus_tag="VIS73_14315"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5764380.1"
                     /translation="MPGPDRLRALALYRRHAPGYDASARRTMPLRERTIARLGLRSGQ
                     TVLDVGAGTGLSFDLLRAGVGDSGQVVGVELSPEMIALARRRVAD"
     gene            474..>2218
                     /locus_tag="VIS73_14320"
     CDS             474..>2218
                     /locus_tag="VIS73_14320"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY5764381.1"
                     /translation="MNGSDGTPRSSIVGARAGGLRVALIVLAVASLLWLAIEVWAPAA
                     LGLGDWRGHHDNFVELVRSHPLEAMLTVFLLHTLLAALALPGASLLMLATGSAYGSWT
                     GTLICLTACTVGATLTMLAGRHLLRPVVIRRWGEQLAKVDARLAEDGPAYLFSLRLLP
                     VVPFAVVNVAAGLGGMKTWTYFWISFVGMAAGTFVYVNAGTELGHIDSFGDIFTPTTL
                     ISLALVALVPWAGKLVSLRLQRPAGSRTLLLFNYDWDAIGFGRWRREFPADTAGFDLF
                     TFPSNARLAGFDLTRFVDRLAARAKRKGWQAVSSNHEQFGALAAAMLAEKMGWPGTPV
                     QAVLACQHKLHARRVLQAIAPEASVPARLMPARYGEPIPEGIDYPAFVKPVKAAFSVL
                     ARTVHSHAELTALTRFGAWELWVIRHLVEPFERIAAVRLPEAGSAHRMMLEAPAPGLQ
                     YNLDGYVFNGEVRTLGFVESAMYPGTQAFMRFAYPCPVPDPVRARALSVAQRFLAAVG
                     FTHGLFNMEFFYDAASDRLTVIEFNPRMAAQFSDLYRRVDGVDLHRVGLELAYGRDPA
                     GLPRLTSTAGAAASFV"
BASE COUNT          337 a          758 c          737 g          386 t
ORIGIN      
        1 gtcggcgacg cgtcgccgcg ccagtgcgat catctcaggc gagagttcga caccgacaac
       61 ctggccactg tcgccgactc cggcgcgcag caggtcgaag gagaggccgg tgccggcgcc
      121 tacgtcaagc acggtctgcc ccgaccgcag cccgaggcgg gcgatggtcc gttcgcgcaa
      181 gggcattgtc cgccgcgccg aggcatcgta gccgggcgca tggcggcggt agagagcgag
      241 tgcgcggaga cgatcaggcc cgggcatcgg ttacgtcggt gggattcatg tcatcgcatc
      301 cttgtaagga agccttggct gtctcgactg ttaaccggaa cgaatcagca agaatccgat
      361 caagagcagg ggaacgcacg cagtatcgca tcgtgaacga atcgggatgg cacgaccggc
      421 cagcgtttcg tcagggggac atccgttgaa agaaagacgt tttggctagg accatgaacg
      481 gcagcgacgg cacgccgcgc agcagcatcg ttggcgcccg tgccggcggc ctgcgcgtgg
      541 cgctgatcgt gctcgcggtg gcctcgctgc tgtggctggc gatcgaggtc tgggcgccgg
      601 ccgcgctcgg gctcggcgac tggcgtggcc accacgacaa cttcgtcgaa ttggtgcgca
      661 gccatcccct cgaggcgatg ctcacggtct ttctgctgca tacgctgctc gccgcattgg
      721 cccttcccgg cgcgtcgctg ctgatgctgg cgaccggttc ggcctacggc tcgtggaccg
      781 gcacgttgat ctgcctgact gcctgcacgg tcggcgcgac gctgaccatg ctcgccggcc
      841 gccacctgct gcggccggtg gtcatccgcc gctggggcga gcaactggcc aaggtcgacg
      901 cgcggctcgc cgaagacggg ccagcctacc tgttcagcct gcgcctgctg ccggtggtgc
      961 cattcgccgt ggtcaacgtc gccgccggac tcggcggcat gaagacctgg acctacttct
     1021 ggatcagctt cgtcggcatg gccgccggca ctttcgtcta tgtaaacgcc ggcaccgaac
     1081 tcggccacat cgacagtttc ggtgacattt tcacgccgac aacgctcatc tcgctggcac
     1141 tggtggcgct cgtgccctgg gccggcaaac tggtctccct ccgcctgcag cggccggccg
     1201 gctcgcgcac gctgctgctc ttcaactacg actgggacgc gatcggcttc ggccgctggc
     1261 gccgggaatt cccggccgac acggcgggct tcgacctatt cacgtttccc agcaatgcgc
     1321 ggctcgccgg cttcgatctg acgcgcttcg tcgaccgcct cgcggcgaga gcgaagcgca
     1381 agggctggca ggcggtcagt tccaaccacg agcagtttgg cgcactcgcc gcggcgatgc
     1441 tcgccgagaa gatgggctgg ccgggaacgc cggtacaggc cgtacttgcc tgccagcaca
     1501 agctgcacgc acggcgcgtg ctgcaggcga tcgcgcccga ggccagcgtg ccggcgcggc
     1561 tgatgccggc acgctacggc gaaccgattc ccgaggggat cgactacccg gccttcgtca
     1621 agccggtgaa ggcagccttc tcggtgctcg cgcgaaccgt ccacagccac gccgaactga
     1681 ccgcgctgac gcgtttcggc gcctgggagc tatgggtgat ccgtcatctg gtcgagccct
     1741 tcgagcggat cgccgccgtg cgcctgcccg aagccggtag tgcgcaccga atgatgctcg
     1801 aggctccggc tccaggcctg cagtacaacc tcgacggcta cgtctttaat ggcgaggtac
     1861 gcacgctcgg cttcgtcgag tcagcgatgt atccgggcac ccaggccttc atgcgcttcg
     1921 cctacccctg cccggtgccc gatccggtgc gtgcgcgggc gctgtcggtg gcgcagcgct
     1981 tccttgccgc ggtcggcttc acgcacggcc tcttcaacat ggaatttttc tatgacgcgg
     2041 ccagcgaccg gctgaccgtt atcgaattca acccgcgcat ggcggcacag ttctccgacc
     2101 tctatcgccg tgtcgatggc gtagacctgc atcgcgtcgg cctcgaactg gcctatggcc
     2161 gcgatccggc gggactgccg cgcctgacct caacggccgg cgcggcagcg agcttcgt
//
LOCUS       DATDAA010000470         2688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Rhodocyclaceae bacterium isolate SMAG_U84 k141_68799,
            whole genome shotgun sequence.
ACCESSION   DATDAA010000470 DATDAA010000000
VERSION     DATDAA010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962287
            Sequence Read Archive: SRR25158264
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodocyclaceae bacterium (soil metagenome)
  ORGANISM  Rhodocyclaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 18:40:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,826
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Rhodocyclaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68799"
                     /isolate="SMAG_U84"
                     /isolation_source="soil"
                     /db_xref="taxon:1898103"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..154
                     /locus_tag="VIS73_14325"
     CDS             11..154
                     /locus_tag="VIS73_14325"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY5764382.1"
                     /translation="MSAVALLEENPRPTREEARQAMSGNLCRCGAYDHYLNGIMRASG
                     QES"
     gene            151..2562
                     /locus_tag="VIS73_14330"
     CDS             151..2562
                     /locus_tag="VIS73_14330"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY5764383.1"
                     /translation="MSDYRLIGKDFTPPDITGKVTGKAKYAEDFRVDGMVFARMYTSP
                     MAHGKVTRIDTSAAEAMPGVVGILTADDVPAVEAPSAAILTNTPAYIGDPILAVAAET
                     EQIAEDAMAVIRVEMEPLPFVTDPLDSLVAGGPNAREEGNVYSRSSEGSGFRTIKWTP
                     EQIDEFRAGREPGGEYVNAWSYGELEKGFADAELVVEEPFVTIGYAHMSMEPRTAMAY
                     WENGKCYVYGSAQSQSFWMPGLARLLEVETKDVCLISEYTGGGFGSKIGAYPVAALPG
                     WFSKKIGRPVQLRVTREQEYYMGSARVGFQGWIKMGFAADGHITATDVFIVEDIGPNA
                     TGGDASSAGGAISLVYDPQAMRFRGLPVMTNTTPRGAQRGPGQNQIAAVMAPIMDKAA
                     KALDIDPVEIRKINSTSNDTIVYADRRPVTSAYMREALDKGAQLFDWENRKNRPKRNG
                     SKVRGLGIGQGYHSAGATGFDGLLRITPDGKLHIHTGVGNLGTYSYASTSRAAAEVLK
                     CDWEKCEIHRGNTEADLPWNSYQAGSVSIFTHTRTNYVAAMDILDKMKRIAADTMGGE
                     PDDYDIDGERVFRIDNEAQGMSYGAIAARAIQLGGEFSGETYPEDINEITQRSVRNLA
                     GSGLVGVAKDNLPQDGVIPGLAVAFCEIELDLETGKYQILDYVGIAECGTVVHPKGLD
                     QQMKGGAVWGMGMAGLERHVYDPQNGLPANVGYHQCRIPTYLDVPSVQHSAAVDLPDP
                     QNPFGARGIGEPSMGCSVAAVQSAISDALDGHIFGRSPISPDMIVNHVAGRDQSYLPL
                     QTNNF"
BASE COUNT          524 a          887 c          880 g          397 t
ORIGIN      
        1 gggacaggtc atgtcggccg tggcgctgct cgaggagaac ccgcgcccga cgcgagagga
       61 agcacgccag gccatgtccg gtaacctgtg ccgctgtggg gcatacgacc actacctgaa
      121 cggcatcatg agagcatcgg ggcaggaatc atgagcgact atcgactgat cggaaaagat
      181 ttcacgccgc ccgacatcac gggcaaggtc acgggcaagg ccaagtacgc ggaagacttt
      241 cgcgtcgacg gcatggtgtt cgcgcgcatg tacacgagcc cgatggcgca cggcaaggtg
      301 acgcgcatcg acaccagcgc agccgaggcc atgcccggcg tcgtcggcat cctgaccgcc
      361 gatgacgtgc ccgcggtcga ggcgccttcc gccgccatac tcaccaacac gccagcctat
      421 atcggcgacc cgattctcgc ggtcgccgcc gagaccgagc agatcgcgga ggacgcgatg
      481 gcggtcatca gggtcgaaat ggaaccgctg cccttcgtca ccgatcctct cgacagcctc
      541 gtcgcgggcg gacccaacgc ccgcgaggaa gggaacgttt actcgcgttc cagcgagggc
      601 tccggtttcc gcacgatcaa gtggacgccg gaacaaattg acgaattccg cgccggcagg
      661 gagcccggcg gcgagtacgt caacgcatgg agctacggcg aactggagaa gggcttcgcc
      721 gacgccgaac tcgtcgtcga ggaacctttc gtgacgatcg gttacgcgca catgagcatg
      781 gaaccgcgca cggctatggc ttactgggaa aacggcaagt gctacgtcta cggctcggcg
      841 caaagccaga gcttctggat gccgggcctg gcccggctcc tcgaggtgga gaccaaggac
      901 gtctgcctga tcagcgagta caccggcggc ggcttcggct ccaagatcgg cgcctatccc
      961 gtcgccgccc tccccggctg gttctcgaaa aagatcggtc gtcccgtgca gctgcgggtg
     1021 acgcgcgagc aggaatacta catgggctcc gcgcgcgtgg gcttccaggg ctggatcaag
     1081 atgggcttcg ccgccgatgg ccacatcacc gccacggacg tgttcatcgt cgaggacatc
     1141 gggccgaacg cgaccggcgg cgacgcctcc tcggcaggcg gcgcgatttc gctggtttac
     1201 gatccccagg ccatgcgctt ccgcggcctg cccgtcatga ccaacaccac gccgcggggc
     1261 gcccagcgcg gtcccggcca gaaccagatc gccgccgtca tggcgccgat catggacaag
     1321 gccgcgaagg ccctggacat cgacccggtt gagattcgca agatcaattc caccagcaac
     1381 gacacgatcg tgtatgccga ccggcgcccg gtgaccagcg cctacatgcg ggaggcgctg
     1441 gacaagggcg cgcagctttt cgactgggag aaccgcaaga accggccgaa gcgaaacggc
     1501 agcaaggtgc gggggctcgg catcggtcag ggctaccact cggccggcgc cacgggcttc
     1561 gacggcctgc tgcgcatcac cccggacggc aagctgcaca tccacacggg cgtcggcaac
     1621 ctgggcacct attcctatgc ctcgacctcg cgcgcggcgg ccgaagtgct gaagtgcgac
     1681 tgggagaaat gcgaaatcca ccgcggcaac acggaagccg acctgccgtg gaactcctac
     1741 caggcgggca gcgtgtccat cttcacccac acgcgcacca attacgtcgc cgcgatggac
     1801 atcctcgaca agatgaaacg catcgcggcc gatacgatgg gcggcgagcc ggacgactac
     1861 gacatcgacg gcgagcgcgt cttccgcatc gacaacgagg cgcagggcat gtcctacggg
     1921 gcaatcgccg cgcgcgccat ccagctgggc ggcgaattca gcggggagac gtatcccgag
     1981 gatatcaacg agatcaccca gcggtcggtc aggaacctgg ccggctcggg acttgtcggc
     2041 gtggcgaagg acaacctgcc gcaggacggc gtgatacccg ggcttgccgt cgcgttctgc
     2101 gagatcgagc tcgatctcga gacgggcaag taccagatcc tcgattacgt gggtattgcc
     2161 gaatgcggca cggtggtgca ccccaagggt ctcgaccagc agatgaaggg cggcgccgtc
     2221 tggggcatgg gcatggccgg cctcgaacgg cacgtatacg acccgcagaa cggtctgccg
     2281 gccaacgtcg gctaccacca gtgccggatc ccgacctacc tcgacgtgcc gtcggtgcag
     2341 cactcggccg cggtcgacct gcccgacccg cagaacccgt tcggcgccag gggcatcggc
     2401 gagccgtcga tgggctgctc ggtcgccgcc gtgcagtcgg cgatctccga cgcgctcgac
     2461 gggcacatat tcggcagatc gccgatcagt ccggacatga tcgtcaacca cgtggcgggc
     2521 cgcgaccagt cgtacctgcc gctgcaaacc aacaacttct gaggtaatca tggcttcttt
     2581 cgacgtaatg ccagaagttc agctcttcca gcctgtggag ctggacgacg cgctcaggct
     2641 cgccgatcgc tacggcaagg acgggtggat actgggcggg ggtcagga
//
